LOCUS       DASYVO010000001         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_268064, whole genome shotgun sequence.
ACCESSION   DASYVO010000001 DASYVO010000000
VERSION     DASYVO010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268064"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VGR03_00005"
     CDS             <1..244
                     /locus_tag="VGR03_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEV1992694.1"
                     /translation="TMYSTTWCPDCRRAKSFLKERGVDFREVDIEEDPSGEEIVIKAN
                     DGKRIVPTLEVGGRYFACSPFDPEQLAAELNIPLNP"
     gene            complement(269..1435)
                     /gene="nagA"
                     /locus_tag="VGR03_00010"
     CDS             complement(269..1435)
                     /gene="nagA"
                     /locus_tag="VGR03_00010"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV1992695.1"
                     /translation="MSTILIHAGRALTPTIEINDAGILIREDVIEAIGQRAGMSLPAG
                     AQEVRATDKTAIPGFIDVHIHGAGGHDVMEGSGAALRAITRKVSEYGTTSVVATTVTA
                     STDLTLRAVEGIAGYIAHQHETDEPRAEILGIHLEGPFISKERRGVHPLEWIQLPSAA
                     MLNRFLKAAAGNARILTIAPEVLGAAPCIDAAREAGLVVSMGHTDATYEQARAAMARG
                     ARSATHVYNAMRPFSHRDPGVIGAVLTSPDVNAELIADGVHVEEAAMKLLLLAKGAAH
                     VTLVSDGLSATGMPDGKYTLGGFEVTVSGGVCRNAEGVLAGSTLTLDRALRNIVALGA
                     SLSDAVRMLTLNPASLLGIEFKKGSLRVGADADILLLDEGLHITNVWARGVALN"
     gene            complement(1527..1856)
                     /locus_tag="VGR03_00015"
     CDS             complement(1527..1856)
                     /locus_tag="VGR03_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992696.1"
                     /translation="MKLRLATGMAVTLFAAALAMAADGTWTGYISDDHCGAKGMSEKH
                     AECATKCVKEKGAKYVFVNDADKKVYAIDAQDKVAAHAGHHVTVKGSVEGDTLKLSGI
                     DMAPAAK"
     gene            2036..>2263
                     /locus_tag="VGR03_00020"
     CDS             2036..>2263
                     /locus_tag="VGR03_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992697.1"
                     /translation="MGRKGKWEYFRAIYGRYHKADRIAKQLMLDEFCLTTGYHRKYAI
                     RILNGPPPGKRLARPERRRGLSYGHELLSILT"
BASE COUNT          439 a          702 c          644 g          478 t
ORIGIN      
        1 caccatgtac agcaccacct ggtgccccga ttgccgccgt gcaaaatcct tcttgaagga
       61 acgcggtgtc gattttcgcg aagtggatat tgaagaagat ccgtccgggg aagagatcgt
      121 catcaaggca aatgatggca agcgtattgt ccccacgctg gaagtaggcg gccgttattt
      181 tgcctgcagc ccgtttgatc cggagcagct cgccgccgaa ctcaacattc cgctcaatcc
      241 gtgaagctgc ggagatttgt ttcgctttct aattcagcgc taccccgcgc gcccagacgt
      301 tggttatgtg caatccttca tccagcagca aaatatccgc atccgcgccc acgcgcaggg
      361 agcccttctt gaattcaatc cctagcaatg acgccggatt caaggtcagc atccgcacgg
      421 cgtccgagag cgaggctccc agcgccacaa tatttcgcag cgcgcgatcc aacgtcagcg
      481 tgctgcccgc cagtactcct tcggcattcc ggcagacgcc gccgctgacc gtcacttcga
      541 agccgcccag tgtgtacttt ccgtccggca tgcccgtcgc ggagaggccg tcgctgacca
      601 gggtgacgtg cgcggccccc ttggcgagga gaagtaattt catcgccgct tcttcgacgt
      661 gcactccgtc ggcaatcagc tccgcattga catccggcga tgtgagcacg gcgccgataa
      721 cgccaggatc gcggtgggaa aacggacgca tcgcgttgta gacatgggta gcgctgcgcg
      781 cccctcgggc catggccgca cgagcctgct cgtatgttgc gtcggtgtgc cccatcgaaa
      841 cgaccaggcc ggcttctcgc gcggcatcga tgcacggagc ggcccccagc acttccggcg
      901 caatcgtcag gatcctggca ttccccgcgg cggccttgag gaagcggttg agcatcgccg
      961 ccgacggcag ctgaatccat tccagtggat gaactccgcg gcgctccttg ctgatgaaag
     1021 gcccctccaa atggattccc aggatttccg cgcggggttc atcggtttcg tgctgatgag
     1081 cgatgtaccc cgcgattcct tcgaccgcgc ggagagttag gtccgtgctg gccgttaccg
     1141 tagtggcaac aacggaggtt gtaccgtatt ccgagacctt gcgagtgatg gcgcgcaacg
     1201 cggcgccact tccctccatc acgtcgtgcc cgcccgcgcc gtggatgtgc acatctataa
     1261 atcccgggat tgccgttttg tccgtagcgc gaacttcctg cgctccggcg ggcaggctca
     1321 tgccggcgcg ttgcccgatg gcctcaatca cgtcctcacg gatcaggatg cccgcgtcgt
     1381 tgatttctat cgtcggagtt agggcacggc cagcgtggat aagaatggtc gacatggcag
     1441 gacaacatcg taacggaaat gattcaccat acaaaacaaa acgggcggag ctgttgcccc
     1501 gcccgccgaa aataaactga caattattat ttggctgctg gcgccatgtc gatgccgctc
     1561 agcttcagcg tgtcgccttc cacgctgccc tttacggtta catgatggcc ggcatgcgcc
     1621 gccactttgt cttgcgcatc gattgcgtac accttcttgt cggcgtcgtt cacgaacacg
     1681 tacttggcgc ctttttcttt cacgcacttc gtcgcgcact cggcgtgctt ttcgctcatc
     1741 cccttggcgc cgcaatggtc atcggagatg tacccggtcc aggtgccatc cgccgccatc
     1801 gccagtgccg ctgcgaagag cgttactgcc attccagttg ctagacgtag tttcatgatt
     1861 tctccctcca gaaatccact gcggtttcta aatccgccgg gccgccccgt agcttggcac
     1921 agtgccggcc cggtgtccag actgcgtatc gagccatcta aaatgtaacc gaaggggaca
     1981 acgtcgcgcc atttaagatg taacccgagc cagagacgat ttggggaggg tttacttggg
     2041 gcgtaagggg aaatgggagt atttccgggc catctatggg cggtatcaca aagcagatcg
     2101 tatagcgaaa cagctgatgc tggacgaatt ttgcctcacc acgggttacc accgaaaata
     2161 cgccattcga attttgaatg gcccgccacc cgggaagcga ctggctcggc cggaacggcg
     2221 gcgggggctg agctatgggc acgaactact gtccatcctg aca
//
LOCUS       DASYVO010000002         8744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_197564, whole genome shotgun sequence.
ACCESSION   DASYVO010000002 DASYVO010000000
VERSION     DASYVO010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8744
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197564"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            713..1651
                     /locus_tag="VGR03_00025"
     CDS             713..1651
                     /locus_tag="VGR03_00025"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV1992698.1"
                     /translation="MRTALANFGVRRFAAAFLPAGAQPGAAIAPAWRDDMKRRELLTH
                     RDYLEAAVEIAQEAGKILVEELSRPLDIAYKGDEVDLVTQADKRSEKLIVERLTKFFP
                     DHAIAAEEGMGHESASASEFRWHVDPLDGTTNFAHGYPCFCVSIALAQRDTLLAAVVF
                     NPFYNELFTAARGAGATFNGKRIHVSLNATLSTSLLCTGFPVRNRKASPNLQYYGDFT
                     QRSHGVRRDGSAALDLASVAAGRFEGFWEFGLQKWDTAAGVLLVEEAGGRVSDFAGNP
                     YQLGGPIILATNSLIHEEMRAMALEISLRTQKVSHS"
     gene            1674..2366
                     /gene="mtnB"
                     /locus_tag="VGR03_00030"
     CDS             1674..2366
                     /gene="mtnB"
                     /locus_tag="VGR03_00030"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: protein motif:HMM:TIGR03328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HEV1992699.1"
                     /translation="MKKTKTSVNRRNSRKANVPSSPNSTRFAEAAARLADIGKGFHTR
                     GWVLGTSGNFSAVISREPLRLAITSTGLDKGSLTPAQFLEIDAAANVLRGDGRPSAEA
                     LLHLAIARGVDAGAVLHTHSVWSTLLSSSHASQGGIALEGYEMLKGLEGVRTHKHREW
                     LPILENSQDMVELGERVSKILRESPGIHGFLLREHGLYTWGAGLQEAKRHVEILEFLM
                     EVLVRSGAAQSA"
     gene            2401..2967
                     /locus_tag="VGR03_00035"
     CDS             2401..2967
                     /locus_tag="VGR03_00035"
                     /inference="COORDINATES: protein motif:HMM:NF015065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1992700.1"
                     /translation="MAVLRIPDENRSLREHDEVRDYLAGIGIDYERWESPADLPSDAS
                     ADQVLSAYSEQVEKLKRQGGYVTADVIDVNADTPGLDAMLAKFNIEHTHDEDEVRYII
                     AGRGLFHIHPQRGPVVAIEVEAGDLIRVPRGTLHWFDLCGDRRIRAIRLFQDKAGWTP
                     HYTSSGVDRNYEPVCWGPRYIPPQTAAK"
     gene            2973..3722
                     /gene="mtnC"
                     /locus_tag="VGR03_00040"
     CDS             2973..3722
                     /gene="mtnC"
                     /locus_tag="VGR03_00040"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR01691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HEV1992701.1"
                     /translation="MAPIDASQIRVILLDIEGTTTPVDFIYQTLFPYASRKLESFLRE
                     HAQDPEMQALVQDLHAQYELDERNGLKPPGWLDHPEEARLRSSVAYAQWLIARDSKCT
                     PLKSLQGKIWQQGFASGELRGEVYPDVPIAFGRWRRQKKIICIYSSGSVLAQQLLFRT
                     TTSGDLTSYISVFFDTRVGAKTEGASYKKIAASFSYAPRQFLFISDAVKEIEAAQSVG
                     MQALLCGREEHPRPTSDAAGVIHDFSSVFPD"
     gene            complement(3798..4937)
                     /locus_tag="VGR03_00045"
     CDS             complement(3798..4937)
                     /locus_tag="VGR03_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1992702.1"
                     /translation="MIDIFSRGHEAVSPGTFSRRSFLVKTSCFGAFYAVAKLIPLPAL
                     AAELTGDARVSQTPIVDKGFAAVRKVGNGLYATISDPSKGNTTLCNGGFLAGKDGALL
                     IEGFTTPAGASFQMDALRMVSQVPVKGALDTHYHFDHSMGNSFYGANGVQLWAHATAA
                     KRIVENYAPLQGADKEAALGPYEKRVKDAKSDVERRHAQSDLNAVTGVFQIANATVLG
                     LPNRPIDPAKLPMSIDLGGLTAVLESYPGHSGTDMIVRVPDQNIVYTGDLLFSGWYPV
                     CFDEKATISGWHDTLKKFAAFDKDTLFVPGHGQICGQEGIASIREVFDDIAGQAEKMY
                     KAGVPAEEAQHRYVVPEKFKNFPVFAWGFTIGPAITKLCAEWAAR"
     gene            5199..5717
                     /locus_tag="VGR03_00050"
     CDS             5199..5717
                     /locus_tag="VGR03_00050"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1992703.1"
                     /translation="MTKITGTVALAIGLLIAPSWQAQESYRDACIQRCEGRPGLGDPE
                     VQRQEIVSLEKEAARAIQLNNGTYFQRVYSDDFAGTLSHGQLVNKTQWIGVIESPAVK
                     YESFNASDIKVRVFEYTAVATCLWSARSIIRGQHISSQIRAIHVYVDTPRGWHVVSAQ
                     TTNLPPDVQQPL"
     gene            5797..7107
                     /locus_tag="VGR03_00055"
     CDS             5797..7107
                     /locus_tag="VGR03_00055"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEV1992704.1"
                     /translation="MRAVDLIRKKRDSGELSREEIDFLVSGYTRGEIPDYQMAAWLMA
                     VWIRGMNRPEIEALTEVMLCSGEVLNHSEIPGKKVDKHSTGGIGDKTSLILAPIVAAG
                     GLTVPMISGRGLGHTGGTLDKLEAIPGFNVNLSLDEFRRVLRECGMGLIGQTAQIAPA
                     DKKIYALRDVTSTVENIGLICASIMSKKLAEGIDALVLDVKTGSGAFMKKEEDAIRLA
                     EVMVETGKRMGKKVVALITGMDQPLGRMAGHSNEVTESIEVLKGRGPADLRDLSLDLS
                     AWMFHLGERTKSVEEGRRLAETMIATGQALEKFRQGIRLQGGDERVIDEPGRLPTARL
                     QFHVASSTSGFVTGTNCEQFGIALAMLGGGREKKEDKIDHSVGLEFHKRIGDRVEKGE
                     PLATIHYNSDAKLAEAKSRIADSFHIGEEAPREKRPLVRRLIGA"
     gene            7133..8365
                     /locus_tag="VGR03_00060"
     CDS             7133..8365
                     /locus_tag="VGR03_00060"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEV1992705.1"
                     /translation="MGRFTGVLGMAVILGLAWVFSTNRRAIKLKTIAWGLGLQLALGF
                     FVLRSSVGSSVFGFLGAGANKLLSFSYAGSAFVFGDIGLPKELSRLGFSFAFQVLPTI
                     MFIAAFFAVLYHLGVMQLIIRAAAWIMTRVMGASGAESLNIAASIFMGQTEAPLTIRP
                     FLPTLTKSELMVVMTSGMAHISGGMMAGYIQVGGADPKNLLTAVIMTAPGTLLMAKML
                     VPETEQPLTAGRVEMPPMEKEANLLGAIARGTIDGLHLALNVGAMLITFIALLALVNA
                     LMGGVHNWPHMAWFPESLQTVFGWIFAPVAWLIGVPWHEAGKIGNLLGTRMVINEFVA
                     FGQLGLMKDSLDPRTFAIATFALCGFANFSSIGIQIGGIGALAPEQRTQLAKFGIRAM
                     LAGTMANLMSAAIVGILS"
     gene            8362..>8744
                     /locus_tag="VGR03_00065"
     CDS             8362..>8744
                     /locus_tag="VGR03_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992706.1"
                     /translation="MSAKKSSKTKIPKTNTSSTSGSSEHEFERAGEAADFIFSQTGLR
                     PQIALVLGSGLGAFADEFADATKIPYAEIPHFPQSTAIGHDGKLVIGRVGEIPVAGMQ
                     GRVHLYEGYSAKEVAFPIRVFARMGV"
BASE COUNT         1789 a         2638 c         2436 g         1881 t
ORIGIN      
        1 cattgccacg gagacgtgtc tcttgacatc gccttctctc ttatcgaggg tggcccggac
       61 ctaaagtaca gagatgaaag ggggcgccca acacgtcggt ttatgtgtgg ggcttttgac
      121 atttcctccg tcttcttgcc ctacccgctt ttattaagaa ttttcgcttt tccgccccgc
      181 atccccattc cccaaatatc gctctctaat cctttaaccc cctaactttt caacaccttc
      241 ccgctccatt cgaatgatat ccaataatta tattgacata cagactactc tctgctattc
      301 tttcccgtgt ctgccaagcc ccaacctcgc cgatctcttg attctttctc cagtctaccc
      361 cctactccct actccctgcc ccctatctcc taactcccat ggaatcatat cgtttgccga
      421 cccccacctt ctaacccttt tggaatcata tcgtttcaaa aacatagggg ggagagggcg
      481 cctccacctt tccaacgctc atcccctaat ccctatcccc tatcccctct cttttcatac
      541 tcttgtgcac tcttttgcac ttttttgcac tcatgcaaaa ctcaactctt ttgttttcaa
      601 tcgattccgc actctgcgtc aaaaaaccga ccggcgtgag gggagggttc agcgctcact
      661 ctcctcagca atcgaacctg cgttgctgcc aatggtaaag tggaggtcgc ccttgcgcac
      721 cgcactggcg aattttggag tgcggcggtt cgccgccgct tttcttcccg cgggggcaca
      781 gcccggtgcg gcgatcgctc cagcttggcg agatgacatg aagagaaggg aactcttgac
      841 acaccgagac tatcttgaag ccgccgttga aattgcccag gaagccggga aaatcctggt
      901 ggaagaattg tcgcgcccgt tggacatcgc ctacaaaggc gacgaagtgg atcttgtcac
      961 ccaggcggac aagcgctcgg aaaagctcat cgtcgagcgg ctgaccaagt tctttcccga
     1021 ccacgccatt gctgccgaag agggaatggg gcatgaaagc gcttcggctt cggaatttcg
     1081 ctggcatgtc gatccgcttg acggcaccac gaatttcgcc catgggtatc cgtgcttctg
     1141 cgtctcgatc gctctagccc agcgggatac gcttctcgct gcggtggtct tcaatccctt
     1201 ttacaacgaa ctcttcaccg ccgcgcgcgg cgcaggcgcc acattcaatg gcaagagaat
     1261 ccacgtttcc ctaaatgcca cgctctcgac cagcctgctc tgcaccggct ttccggtgcg
     1321 caaccgcaag gccagcccaa atcttcaata ctacggcgac ttcacccagc gctctcatgg
     1381 agtgcgccgc gacggctccg ccgcgctgga cctcgcctcc gtcgctgccg gccgcttcga
     1441 gggcttctgg gaattcggcc ttcagaaatg ggacaccgcc gccggcgtcc tgctggtcga
     1501 agaagccggc ggcagagtca gcgattttgc cgggaatcct tatcagcttg gcggccccat
     1561 cattctcgcc accaacagcc tgatccacga agagatgcga gccatggcgc tggaaatttc
     1621 cctccgcacc cagaaggttt ctcattcatg aaatctcctc gaggggcgcg accatgaaga
     1681 aaaccaagac aagcgtcaat cgtaggaata gccgcaaggc gaacgtgcca tcgagcccaa
     1741 acagtacgcg cttcgcggag gccgccgctc gcctggcgga cattggaaaa ggtttccaca
     1801 cacgcggctg ggttctggga acgagtggaa acttcagcgc ggtgatttcc cgcgagccgc
     1861 tgcgtctggc cattacttca acgggcttgg acaaaggcag tcttacgccc gcgcagtttc
     1921 tggagattga cgccgcggcc aacgttctcc gcggcgatgg ccgcccgtct gccgaagccc
     1981 tgctccatct agccatcgca cgcggtgtgg atgccggcgc agttttgcac acgcattcag
     2041 tgtggagcac tctgctctcg agctcgcatg cgtcacaggg tggcatcgcc ctggaaggat
     2101 atgaaatgct caaagggctg gaaggcgtgc gaacgcataa gcaccgcgag tggctgccga
     2161 ttctcgagaa ttcgcaggac atggttgaac tgggagaacg cgtttcgaaa atcctgcgcg
     2221 aaagccccgg catccatggc tttcttctgc gggagcacgg gctttatact tggggtgcgg
     2281 gcttgcagga agccaagcgc catgttgaaa tcctggagtt cctgatggaa gtactggttc
     2341 gctccggcgc cgcacagagt gcgtgattca cgcgctgccc gagcctggag ataaattgcc
     2401 gtggctgttc tccgaatccc cgatgaaaac cgttcgctcc gcgaacacga tgaggttcga
     2461 gactacctcg ccggaattgg aattgactac gaacgctggg agtctccggc cgacctgcct
     2521 tcggatgctt ccgccgatca agtcctgagc gcctacagcg agcaagtgga aaagctcaag
     2581 cgccagggtg gctacgtcac cgcggacgtg atcgacgtca acgcggatac accgggactg
     2641 gacgcgatgc tggcaaaatt caacatcgaa catacgcacg atgaggacga agtccgctac
     2701 attatcgccg ggcgcggcct cttccatatc catccccagc ggggcccggt agtcgcgatc
     2761 gaggtggaag ccggtgactt gattcgcgtt ccgcgtggga cgctccactg gttcgatctc
     2821 tgcggagacc gccgcattcg cgccattcgt ttgttccagg acaaggccgg ctggacgccc
     2881 cactacacga gcagcggcgt ggaccgcaat tacgaacccg tttgctgggg accccgatac
     2941 attccgccgc aaacggcggc gaagtagccg gcgtggctcc gatcgacgca tcccaaatcc
     3001 gcgtcattct tctcgacatc gaaggcacga caacacccgt cgatttcatt tatcaaacgc
     3061 tcttccccta tgcgagccgc aagctggaat catttcttcg agaacacgct caagatcccg
     3121 aaatgcaggc tttggttcaa gatctgcacg cgcaatacga gttggatgag cgcaatggcc
     3181 ttaagccgcc tggctggctg gatcatccgg aagaagcgcg gctgcgttcg agcgtcgcgt
     3241 atgcccaatg gctgattgcc cgtgacagca agtgcacgcc actgaaatca cttcagggga
     3301 aaatctggca gcagggattc gcgagtgggg aattgcgcgg tgaagtgtat ccggatgtac
     3361 ccattgcttt cggacggtgg cggcggcaga aaaaaattat ctgcatctat tcatccggaa
     3421 gcgtcctcgc gcagcaactc ctgtttcgca ccacgacctc aggcgatctg acgtcgtaca
     3481 tctccgtttt cttcgacacc cgcgtgggag ccaagaccga aggggcaagc tacaagaaaa
     3541 tcgcggcttc gttttcgtat gcgccgcgcc agtttttgtt tatttccgat gcggtgaaag
     3601 agatcgaagc ggcgcagtcc gtgggaatgc aagcccttct ctgcgggcga gaagagcatc
     3661 cgaggccgac gtccgacgct gccggagtga ttcacgattt cagtagtgtt tttccagatt
     3721 aggcggcccg cctccccgcg aagcgcgggg caggcgccag gcgggcccta caagagaatt
     3781 tgacgggggt gggaccgcta ccgggcggcc cactccgcgc acagcttggt gatcgcgggc
     3841 ccgatcgtga atccccacgc aaaaacaggg aagttcttga acttctccgg caccacgtag
     3901 cggtgctgcg cttcttccgc tgggactccc gccttgtaca ttttttcggc ttgcccagca
     3961 atgtcatcaa agacttcgcg gatcgaagcg atgccttcct ggccgcaaat ctggccgtgg
     4021 cccggaacaa agagcgtgtc cttatcgaac gcggcgaatt ttttcaacgt gtcgtgccag
     4081 cccgaaatcg tggccttctc gtcaaagcag acgggatacc agccactgaa tagcaggtcg
     4141 ccggtgtaga caatattctg gtccggcacg cgcacgatca tgtccgttcc ggaatgaccg
     4201 gggtaggact cgagcaccgc cgtcagacct ccgaggtcaa tcgacatggg caactttgcc
     4261 ggatcaatcg gacgattggg caggccaagc acggtcgcat ttgcaatttg gaagacgccg
     4321 gtaactgcgt tcagatcact ctgtgcgtgc cgccgctcta catccgactt ggcatccttg
     4381 acgcgcttct cgtacggccc cagcgccgcc tctttgtccg cgccctgcag cggcgcatag
     4441 ttttcgacga tgcgcttcgc cgctgtggcg tgcgcccaga gttgcacgcc gttcgcgccg
     4501 taaaacgagt ttcccatcga gtggtcaaag tgatagtgcg tgtccaaggc gcccttcacg
     4561 ggcacctggc tgaccatccg cagcgcgtcc atttggaacg acgcgccggc cggcgtcgtg
     4621 aatccttcga tcagcagcgc gccgtctttt ccagccagga agccgccatt gcagagcgtt
     4681 gtgttgccct tcgacggatc ggagatcgtg gcatacagac cgttgccaac tttccgcacc
     4741 gcggcgaacc ctttgtccac aatcggtgtt tgcgacacgc gcgcatcgcc cgtcagctcc
     4801 gccgccagcg ccggcaaggg gatcagcttc gctactgcat aaaaagctcc gaaacacgat
     4861 gtcttgacga ggaagctccg ccgtgaaaac gtaccgggac tgaccgcttc gtggccacgg
     4921 ctgaaaatgt cgatcatgga tcccccgatg aaatgaaaac ttcgattgga tgactgccgc
     4981 tgaggatacg ttattttagc agacacttcc aagggagccg atttccgaac cttccgttga
     5041 ggcggactcc aattggaagc ttgtcattca gaacacgcat gtcaggtatg tgatggatat
     5101 gggcaatctt ctttccgtaa catggagtgt gtccgcggca acccttccct aagttactcc
     5161 ggcggaaatc agtcactcct tgagggaggc actccagcat gaccaagatt acgggtacgg
     5221 tggcattggc aatcggattg ttaattgctc cctcctggca agcgcaagaa tcctatcgag
     5281 atgcgtgcat tcagcgttgt gagggccggc cggggctcgg agatcccgaa gtgcagaggc
     5341 aggagatcgt cagtctcgaa aaagaagccg cgcgcgccat ccagctgaac aatggcacgt
     5401 actttcagcg cgtctacagc gatgacttcg ctggcacact ttctcacggt caactggtaa
     5461 acaagacgca gtggatcggc gtgatagaat ctcctgcggt taaatacgag tcgttcaacg
     5521 cctccgacat caaagtccgc gtttttgaat acacggcggt tgccacttgc ctctggtcag
     5581 cacgcagcat catcaggggc cagcacatca gcagccagat tcgcgccata cacgtttatg
     5641 tggatacgcc aaggggctgg catgtggtct cagcgcagac gactaacttg cctccggacg
     5701 tgcagcagcc gctgtagcgt ttgccggttt cagcgcgcgc atcgcgttga cgcctcgtaa
     5761 agcgaatgtt acgctcgccg ttcgctttcc aaacgcatgc gcgccgtcga cttgatccgc
     5821 aagaaacgtg attccgggga actctcccgc gaagaaatcg attttctggt ttccggctac
     5881 acccgcggag aaatacccga ttatcagatg gccgcgtggc tcatggccgt gtggattcgc
     5941 ggcatgaacc gccccgagat cgaagccctc accgaagtga tgctctgttc cggcgaggtc
     6001 ctcaaccatt ccgaaatccc cggcaagaaa gtggacaagc actccaccgg cgggattggc
     6061 gataagactt ctctcattct tgctcccatt gttgcggcgg gcggccttac ggttccgatg
     6121 atcagcggac gcggcctcgg ccacaccggc ggcacgctcg acaaactgga agcgattcca
     6181 ggcttcaacg tgaatctgtc gctcgacgaa tttcgccggg ttctgcgcga atgcggcatg
     6241 ggcttgatcg ggcaaacggc gcagatcgct cctgccgaca agaagattta cgcgctccgt
     6301 gacgtaactt cgaccgtcga gaatatcggc ctgatttgcg cgtccatcat gagcaagaaa
     6361 cttgcggaag gcatcgacgc tctggtcctc gacgtgaaga ccggctccgg cgccttcatg
     6421 aagaaagagg aagatgcgat tcgcctcgcc gaagtcatgg tcgaaaccgg caagcgcatg
     6481 ggcaaaaaag tggttgcgct catcaccggc atggatcagc cgcttggccg catggccggc
     6541 cattcgaacg aggtcaccga gtccatcgaa gtcctcaaag gccgcggccc cgccgatctg
     6601 cgcgatttga gcctcgatct ctccgcgtgg atgttccatc tcggtgagag aacgaagagc
     6661 gtggaagaag gccgccgcct cgccgaaacc atgattgcca ccggccaggc cctcgaaaaa
     6721 ttccggcaag ggattcgcct gcaaggcggc gatgaacgcg tcattgacga gccgggacgt
     6781 ctgcccaccg cgcgcctgca attccacgtc gcgagttcca ccagcggctt cgtcaccggc
     6841 accaactgcg agcagtttgg catcgcgctc gccatgctcg gtggaggtcg cgaaaaaaag
     6901 gaagacaaga tcgatcacag cgtcggcctc gaattccaca aacggatcgg cgatcgcgtc
     6961 gaaaaaggag aaccgttggc cacgattcat tacaatagcg acgccaaact tgccgaagcg
     7021 aagagccgca tcgcggacag ttttcacatt ggcgaagagg ccccgcgaga aaagcggcct
     7081 ctagtgcgcc gattgattgg cgcttgaatc tgttttttgg agagaggtgt tcatgggacg
     7141 ttttaccgga gtactcggga tggccgtgat tctggggctg gcgtgggtgt tctccaccaa
     7201 tcgtcgcgcc atcaagctca agaccatcgc ttggggtctc gggctccagt tggcgcttgg
     7261 ctttttcgtt ctccgttcca gtgtaggtag ttcggtgttt ggttttctcg gcgccggcgc
     7321 gaataaactg ttgagctttt cctacgcggg ttccgcgttc gtgttcggcg acattggtct
     7381 gccgaaagag ctctcccggc tgggcttttc gtttgccttc caggtacttc ccacgatcat
     7441 gtttattgcc gccttctttg cggttcttta tcacctcggc gtgatgcagc tcatcatccg
     7501 cgctgccgcg tggattatga ctcgcgtaat gggcgccagc ggcgcggaat cccttaatat
     7561 tgccgccagt atttttatgg ggcagacgga agcgccgctt accattcgcc cctttcttcc
     7621 cacgctcacc aagtcggaac tgatggtcgt catgaccagc ggaatggcgc acatctctgg
     7681 cggcatgatg gccgggtaca tccaggtggg gggcgccgat cccaagaacc ttttgaccgc
     7741 agtgattatg acggcgccgg gaacactgct catggccaaa atgctggtcc cggaaaccga
     7801 gcagccgctc accgcggggc gcgtggaaat gccgcctatg gaaaaagaag ccaatcttct
     7861 gggcgccatc gctcggggca ccatcgacgg cttgcatctc gcgcttaatg tcggcgccat
     7921 gctgattacc ttcatcgcct tgcttgcgct ggtcaatgcc ctcatgggcg gcgttcacaa
     7981 ctggccgcac atggcctggt tcccggagag tctgcagact gttttcggct ggatcttcgc
     8041 gccagtggcc tggctgatcg gtgtgccctg gcatgaggca ggaaaaattg gcaacctgct
     8101 cgggacgcgc atggtcatca atgaattcgt cgcttttggt cagcttggcc taatgaagga
     8161 ctcgctcgat ccgcgcactt ttgccatcgc caccttcgcg ctctgcgggt tcgccaactt
     8221 cagctccatc ggcatccaga tcggcgggat cggagcactc gctccggaac aacgcacgca
     8281 attggccaag ttcggcatcc gcgccatgct cgccggcacc atggcaaact tgatgtctgc
     8341 tgccattgtg gggattcttt cgtgagcgca aaaaaaagct ctaagaccaa gattccgaaa
     8401 acgaatacgt cctcaaccag cggttcatcg gaacacgagt tcgagcgcgc aggggaagcg
     8461 gccgacttca ttttttctca gacgggtctg aggccccaga ttgccttggt tctgggttcc
     8521 ggccttggcg ctttcgcaga cgaattcgcc gatgccacca aaatccccta cgcggaaatt
     8581 ccgcattttc ctcaatcgac cgccattggc cacgacggga agctcgtcat cggaagagtt
     8641 ggagaaattc ctgttgccgg gatgcagggc cgcgtccatc tttacgaagg ctattccgct
     8701 aaagaggtgg cctttcccat ccgcgttttc gcgcgcatgg gcgt
//
LOCUS       DASYVO010000003         4543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_225660, whole genome shotgun sequence.
ACCESSION   DASYVO010000003 DASYVO010000000
VERSION     DASYVO010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225660"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            552..627
                     /locus_tag="VGR03_00070"
     tRNA            552..627
                     /locus_tag="VGR03_00070"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:585..587,aa:Asn,seq:gtt)
     gene            complement(661..1032)
                     /locus_tag="VGR03_00075"
     CDS             complement(661..1032)
                     /locus_tag="VGR03_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1992707.1"
                     /translation="MAHAAQFLKLVNEAKKKIKETNVAEVKRRTDAGEKLLLVDVRED
                     NEWAKGHLPGAVHMGRGIIERDIEQRVQDTNAKLVLYCGGGFRSALVAENLQKMGYNN
                     VESMDGGWKGWLEAGLPIARE"
     gene            complement(1161..1625)
                     /locus_tag="VGR03_00080"
     CDS             complement(1161..1625)
                     /locus_tag="VGR03_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEV1992708.1"
                     /translation="MRRTVLVLSAILLASFGVLYAKPNSTQDKPGSTEPKMTPEDAAK
                     KNPVPPTPEGLAEVRKLFGYNCAMCHGKTGDGKGDLAADMKLELRDWRDASSIEQMTD
                     GELFWIISNGKGKMPGEGDRTPAKARWNFVNLVRSFAKKGAGDKPKAETPPS"
     gene            complement(1761..4388)
                     /gene="polA"
                     /locus_tag="VGR03_00085"
     CDS             complement(1761..4388)
                     /gene="polA"
                     /locus_tag="VGR03_00085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV1992709.1"
                     /translation="MPSAPKKLFLVDAMAHIYRAFFAPMNRMNAPSGIPTKVPFLFSN
                     ILRRLIKDYQPDYLGIVFDTKGPTFRDKLFEKYKAQRPPMPDDLSVQIPYVRKLCEAM
                     HLPILEFQGYEADDVIGALATQGAKKQLEVLIVSNDKDMMQLVGKSVRTLRTGSGGAK
                     ADIIVDEKKVEEILGVPPEKVIDLMALLGDTVDNIPGAKGIGEKGATELIQKYGSVEN
                     ALDHANEVSNKRYREALQQQREQVMMSKQLATIATDVPLELDLHALELRAPDVNALAA
                     LYRELGFNSLLRELGSEAVASSAPANSESTTKADYAQFASVTEFREYLAKLPAKQPMA
                     IWLNLEAGERETEGFGTRIASIEVSSKAGEARSVWFDEKGDALKALKPLLVDPKRPKI
                     VHDPKLFQLLTGRAPNIRHATQLYSYLLRPTTANHNFADVVMRQFNVMMGGGPGERAD
                     YLQRLAPTLHAQVQEQQLEGVYEKMDLPLAPVLADIERIGIRVDPQELGKMSQTMEKE
                     IRRLEKEIWKLAGSEFNVNSPTQLAEILFDKLNLQPNARRGKAKARSTAADILEELSA
                     QHPLPAKIIEYREIAKLKSTYVDALPKLIHPETGRLHTSFSQTGTATGRLSSSDPNLQ
                     NIPIRSELGREIRAAFVAEKGKILLSADYSQIELRIMAHFSGDPVLVEAFRNGEDIHA
                     RTAQEVFGVGPMAQNAEHRRAAKAINFGIIYGLSPFGLAHQLGIEQKEAAKFINAYFT
                     RYRGVKEYLDNILAETRKTGVAKTLFGRIRPIPEINSPQMQLRNFAERTALNSPLQGT
                     AADLIKLAMISIDRRLAEEKFDAKMILQVHDELLFEAPTKERAKLEKLVQEEMEGVHK
                     LAVPIVVEIGAGPNWRDLE"
BASE COUNT          892 a         1323 c         1272 g         1056 t
ORIGIN      
        1 accagaaaga gggtcgaaaa cacactgaat cgcgcgatga aattcgtact gcgatgaaaa
       61 atctgggcca tgtctgttca gcactttgcc ggggagcgcc ccggaaggga gctgcggtca
      121 gggcgtccct cgagttaacc gggaagtgca aacccgggtc gtgcttgacg cgatagtgtc
      181 caacaaaaca ttcgggaatg caacctcagt gccaatttgt cttccgcagt ttccaagatt
      241 ccagcggttg caaattcgcc ttttcattca aatctatcga aaaaaatgtt ttaagtccgc
      301 aagcccaaca tttccgaccc aagagcgcgt cctacggaac ccggtttcta tcccgctgca
      361 ttccggaaat ggcctatttc catcccaaat gggaccatcc ttctggtttt acccgctata
      421 agttttactt agacgcgttc gcgggacgcg cactcgtggt atcttggaag cacacttcgg
      481 cgctcttttt gcggcggagc ctacctatac ctgtgcttcc cctcgccaaa tcgggggtga
      541 agacagcccg ctggggcgtg gtccaactgg taggacacct cactgttaat gaggacggtg
      601 aaggttcgaa tccttccgcc ccagccaatt ctttttgacc cacagcgcag ccggacgccg
      661 ctattcccgc gctatcggca agcccgcttc cagccagccc ttccacccgc cgtccatcga
      721 ttcgacgttg ttgtagccca tcttctgcag attctccgcc acgagcgcgg aacggaatcc
      781 gccgccgcaa tacaggacga gtttcgcatt cgtatcctgg actcgctgct cgatgtctcg
      841 ctcgatgatg cctcggccca tgtgaacggc gccggggaga tgccccttcg cccactcgtt
      901 gtcctcgcga acatccacga gcagaagctt ttcacccgcg tccgtgcgtc gcttcacttc
      961 ggcgacattg gtttctttga tcttcttttt cgcttcgttt accaatttca gaaattgcgc
     1021 cgcgtgggcc atcggaaaac tccttccagg aatgattcga ggcgagcata acacagaggg
     1081 aatgaaccct cgtcccgccc gcggtcattc tggactccaa ttccgtggtt gaaaagaact
     1141 cagacggaag ttgacagcag ctaactcggc ggtgtctccg cctttggttt atcccccgcg
     1201 cccttcttgg caaaagagcg caccaggttc acgaagttcc agcgtgcctt tgccggggtt
     1261 cgatcgcctt cgcccggcat cttccctttg ccgttgctga tgatccaaaa gagttcgccg
     1321 tcggtcatct gctcgatgga agaagcgtcg cgccagtcgc ggagttcgag cttcatgtcc
     1381 gcggccaggt cacccttgcc atcaccggtc ttgccgtggc acatcgcgca attgtagcca
     1441 aaaagtttgc gcacttccgc caagccttcc ggcgttggcg ggacgggatt ctttttggcg
     1501 gcatcctccg gagtcatttt tggctccgtg gatcccggct tatcctgcgt ggaattcggc
     1561 ttcgcatata agaccccgaa gctcgcgagc agaatcgcac tcaggacaag aactgtcctg
     1621 cgcatagttg cctcctgaca ggatgggccg gccgagtgag cttgttcggc ttgattttga
     1681 gcggattcta cgccccgggc gtgcagaagg aaaggatcaa aaaaataaat attgaggttc
     1741 cgttgcgccg gagagaagcg ctattccagg tcgcgccagt tgggcccggc gccgatttcc
     1801 acgacgatgg gcaccgccag tttgtgaacg ccttccattt cttcctgaac tagtttttcc
     1861 agcttggcgc gttcctttgt gggagcctcg aagagcagtt cgtcatgcac ctgcagaatc
     1921 attttagcat cgaacttttc ttccgccagc cgccggtcga tgctgatcat cgccagcttg
     1981 atcagatcgg ctgccgtgcc ctgcaacggc gaattgagcg cggtgcgctc ggcaaagttg
     2041 cggagctgca tctggggtga attgatttca ggaatcgggc ggattcttcc gaacagagtc
     2101 ttcgcgacgc cggtcttgcg cgtctcggca agaatattgt ccagatactc cttcacgccg
     2161 cgatagcgcg tgaaataggc gttgatgaat ttcgcggctt ctttctgctc gatgcccaat
     2221 tggtgcgcga gcccgaatgg cgagaggccg taaatgatgc cgaagttgat ggccttggcg
     2281 gcccggcgat gctccgcgtt ttgtgccatt ggccccacgc cgaacacttc ctgcgccgtg
     2341 cgcgcgtgga tgtcttcacc attgcgaaaa gcttcgacga gcacggggtc gcctgaaaag
     2401 tgcgccatga tgcgcagctc gatctgcgag tagtccgcgg acagcagtat ctttcctttc
     2461 tccgcgacga aagcggcgcg aatctcgcgg cctagctcgc tgcggatagg aatgttctgc
     2521 aaattgggat cactcgaact gagccggccc gtggcagtgc cggtttgcga aaagctggtg
     2581 tgcagacgtc cggtttcggg atggatcagt ttcggaagcg cgtccacata cgtcgatttg
     2641 agcttggcaa tttcgcggta ctcgatgatc ttcgcgggaa gcggatgctg cgcggacaat
     2701 tcctcgagaa tgtcggcggc ggtagaccgt gccttcgctt tgccgcgccg ggcgttgggc
     2761 tgaagattca gtttgtcaaa aaggatctcg gcgagctgcg tcggcgagtt tacgttgaat
     2821 tccgagcccg caagcttcca gatctctttt tccaggcggc ggatttcctt ctccatcgtc
     2881 tgcgacatct tgcccaactc ttggggatcg acgcggatgc caatccgctc aatatccgca
     2941 agcacaggcg cgagcggaag atccattttt tcgtagacgc cttcgagctg ttgctcctgg
     3001 acttgcgcgt ggagtgtcgg cgcaagccgc tgcagataat cggcgcgctc gccgggcccg
     3061 ccgcccatca tcacgttgaa ctgccgcatc accacgtcgg cgaagttgtg attcgccgtc
     3121 gtgggccgca gaaggtaaga ataaagctgc gtcgcatggc ggatattcgg cgcccggccg
     3181 gtaagcaatt gaaagagttt tgggtcgtgg acgattttcg gccgttttgg atcgacaagc
     3241 agcggcttga gtgctttcaa tgcgtcgccc ttttcgtcga accagacgga gcgcgcttcg
     3301 ccggctttgg atgaaacttc gatcgaagcg atgcgcgtcc cgaatccttc cgtttcgcgc
     3361 tcgcccgcct caagattcag ccagatcgcc atcggttgtt tcgcgggaag tttcgccaga
     3421 tactcgcgaa attcggtgac gctggcgaac tgcgcgtaat ccgccttcgt ggtcgattcg
     3481 gaattcgccg gggcggagga cgccacggct tccgaaccca gttcccgaag caaagaatta
     3541 aaaccgagtt cgcgatagag cgccgcgaga gcattcacat cgggtgcgcg gagttccagc
     3601 gcatgcaagt ccagttccag cggcacatcc gtggcaatgg tcgcaagctg cttggacatc
     3661 atcacttgct cgcgctgctg ctgaagcgct tcgcgatatc gcttattcga aacctcgttg
     3721 gcatgatcca gcgcgttctc cacgctgccg tatttttgaa tcagctcggt cgcgcccttt
     3781 tcaccgatgc ctttcgcgcc gggaatgttg tccaccgtgt cgcccagcag cgccattaaa
     3841 tcaattactt tttccggtgg aacaccgagg atttcctcca cctttttctc gtcaacgatg
     3901 atgtccgcct ttgcgccgcc ggaacccgtg cgcagcgttc gaacgctctt tccaaccagc
     3961 tgcatcatgt ccttgtcgtt gctgacgatc aggacttcga gctgtttttt tgcgccttgg
     4021 gtggccagcg cgccgataac atcgtcagcc tcgtaaccct gaaactccag aatcggcagg
     4081 tgcatcgctt cgcagagctt tcgaacgtag ggaatctgga ccgacagatc gtccggcatg
     4141 ggcgggcgct gcgctttgta tttttcaaaa agcttgtcgc ggaaggtcgg ccccttggtg
     4201 tcgaagacga tgcccaggta atccggctga tagtccttga tgagccgccg caaaatgttc
     4261 gagaagagaa acggcacttt agtggggatg ccagaaggcg cattcatgcg attcatcggc
     4321 gcaaagaacg cgcgatagat gtgcgccatc gcatccacaa ggaagagttt cttcggagca
     4381 gaaggcatgc gggcgagtat agcaaacgcc gcatatggag ggcgggcgcc ccgcctccgc
     4441 ttttgagctt gcagctaaga gacgaccgcc aaagaaaaag gccgcacggg aagcacttcc
     4501 agcgcggcct ttcaaagcaa cattcggcct ttattgctcg ttc
//
LOCUS       DASYVO010000004        36720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_14230, whole genome shotgun sequence.
ACCESSION   DASYVO010000004 DASYVO010000000
VERSION     DASYVO010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 36720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36720
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14230"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..1048)
                     /locus_tag="VGR03_00090"
     CDS             complement(329..1048)
                     /locus_tag="VGR03_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992710.1"
                     /translation="MQVIQFARLPFLTILVGLSCIVPGAIHAQAPSGPITAAPSQPAP
                     ATVANAPNPAPAPRTTILGAWKFNPDDSDEASKRRQDSRGSNGGGHGGGHGRMGGGYP
                     GGGHGSYGGRGGESDEERQKMHELFTPARAITLSMTGAEVDLVDDRDRKRAFMTDGRK
                     LQKSKDENYQEIAAKWDGNRLGTEEKDPRGNKMSRTFELSYDGRQLYETLHMTTGRSN
                     APLVICYVYDIPVQTASQTKQ"
     gene            complement(1120..1854)
                     /locus_tag="VGR03_00095"
     CDS             complement(1120..1854)
                     /locus_tag="VGR03_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEV1992711.1"
                     /translation="MPKNSLASTKRAPRRKLLTTPPRILIFDVDGVLVDVRGTYWRSA
                     LDTVRYLTGKRVTYAELHKWKSKPGFNDDWNMVSAWVSSLGHPTSYAEARAAFEKFYW
                     GSDGKPGNVRNEKLIVTPRQIEKWAQSFELNLFTGRTRQEFSFTFEKWPATKFFRTII
                     TMDDAKRKPHPEGLRKILEGRDPSTALYVGDNIDDALAARDASVPFMAIIAPGEHGYR
                     QRAARFRELGALALLPRATDLKAWLK"
     gene            1829..3193
                     /locus_tag="VGR03_00100"
     CDS             1829..3193
                     /locus_tag="VGR03_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV1992712.1"
                     /translation="MDAKEFFGTDPRSEYVSATKQAGKNSGCKTKVSLLATPRVKRAT
                     VAGESGALLHWTGTLRKEPRMAKYGGVDFIDFDSQLNDEEKLVRQTAREFVENEIIPI
                     IEKHSREGVFPKHLVPQLGELGFFGANLHGYGCAGMSNVAYGLMTQELERGDSGLRSF
                     VSVQGALVMYPIYSYGSDAQKDKWLPLLQQGKAIGCFGLTEPQFGSNPGGMLARAVKK
                     GNKYILNGEKMWITSGSIADVAVVWAKTEDDKVRGFLVEKGAPGFKAWDIHGKWSLRA
                     SVTSGLAFTDCEIPEENLLPGVVGLKGPLSCLNQARYGIGWGALGAAMACYDTALQYA
                     KTRKQFANKPIASHQLVQEKLAWMITEIVKGQLLALHVGRLKDSGKVDPSHISMLKMN
                     NVAIALDTARKARDILGANGIVDDYCIMRHMNNLESVFTYEGTNDIHKLVIGERITGI
                     AAYV"
     gene            3314..4597
                     /locus_tag="VGR03_00105"
     CDS             3314..4597
                     /locus_tag="VGR03_00105"
                     /inference="COORDINATES: protein motif:HMM:NF017019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HEV1992713.1"
                     /translation="MRSRATTLTRFLRYSLVTLLVTLAGNCEIVCAQQKSAAAQEKPK
                     IRAITAFINLDRAQYKEQVADALKMLKRARTIFESRGYQVQTIRIATQPFPEYTKGLT
                     KEQTIAFFKEYDALAVKEKFAASIGPAMLNAGGSDSQAELLAEILSNTKTLNASLVVA
                     GADGVRWGAVGAAARVMKKLEETEHSQGNFRFAAIAMVPPLTPFFPAAYQTGFGHQFA
                     IALESANVVAAAFKDAPDLATAKQRLTDLLASSAFDIERHAGRIDQETGWAYMGIDLS
                     PAPSGDVSIGAAIENLTTQPIGMSGTLTAAATITAAVKDVRVKQIGYSGLMLPILEDT
                     RLAQRWSEGHISIDALLSYSAVCGTGLDTVPLPGDVSAEQLSLIIGDMASLAFKWHKP
                     LSARLLPVLGKGWGEMTEFDDPFLVNAKLQSLDIK"
     gene            5207..5854
                     /locus_tag="VGR03_00110"
     CDS             5207..5854
                     /locus_tag="VGR03_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992714.1"
                     /translation="MLVEILEKLKQEWRVIKGAPFAFTFVCVVAVSACLGGFRWFYHE
                     KLTESETLANHWKSEADYWRDVASHPSQTSPPAATQPVQGKPIPPKIVTRTLPAPEFG
                     TQVVHRENFIPGDSKYGSGLRLTLATDQRRGPVQLLIICDGDIGLAPSAGKSSKSGVF
                     VVESQMLMPGHPEVWNVKWRTPEWTPGDTIMFEFLSKKKIDANAVIPITYNPNAQ"
     gene            complement(6243..6797)
                     /locus_tag="VGR03_00115"
     CDS             complement(6243..6797)
                     /locus_tag="VGR03_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HEV1992715.1"
                     /translation="MARTSEKKFRELILLICARSQGDKKFGSIKLNKLLFYGDFSAYL
                     TFGTAITGQEYFKLPKGPAPRKLLPVVEKMQQSGELAYEEIQYFGHTQKKPIALRQPD
                     VSVFTPQEIDLVHTMIQKYWNLGASEISEKSHLFLGWKVARNKETIPYSTALVGSRPP
                     ITEEQAYGLELAPLALDCLTRANG"
     gene            6902..7069
                     /locus_tag="VGR03_00120"
     CDS             6902..7069
                     /locus_tag="VGR03_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992716.1"
                     /translation="MAKEGPLIVICMTRVIPKIELADTSTQELLPYTVVYTVYAPIEN
                     RKVAFNRICAD"
     gene            complement(7342..8886)
                     /locus_tag="VGR03_00125"
     CDS             complement(7342..8886)
                     /locus_tag="VGR03_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1992717.1"
                     /translation="MSKRKEISRRKFLASAAVGSAACAIVPSHVLGRGVTPPSDTLNI
                     AAVGVGGMGRSNLINLASQNIVALCDVDWRYADKGFDHLDIDIERLKKRIELPAPPQP
                     GAVPVPFDAVRAQARVDAMIKLKSTHLPKATRYTDYREMFERQKDIDAVVIATPDHMH
                     ATIALAAMELGKHVYVQKPLCWSVEEARKLSRRARETKVATQMGNQGHSSDDGRTAVE
                     YIWGGAIGDVREVHIWTNRPLGFWPQGVPRPEPLKAGPEPLKWSGRDVNTRLAAALCG
                     DYPVPGGLEWGLFLGAAPNVDYHPIYHPFNWRGWTDWGVGAIGDMGAHLIDHTMWALN
                     LGFPTSVETVSTPFNGVSYPHATLTFYDFPARDPMPPVKLTWYDGGLKPNKPEELGDE
                     ELNKEGGALLIGSKGKLLHDTYGFKPRLLPKSLHDSFGKPPRKLPRIAGEAHEMNWVD
                     VAKGKVEASCPFEYAARLTEVMLLGIVALRAGRKIYYDGANMRITNVLQANDYLRREY
                     RQGWSI"
     gene            9045..9527
                     /locus_tag="VGR03_00130"
     CDS             9045..9527
                     /locus_tag="VGR03_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV1992718.1"
                     /translation="MTLTRESAPSSRRRAVRDILLYGVAGGVLIAVLKLTEYRFLVVE
                     HSVEIYGALIAALFAGLGIWLGQTLTRKKPAVIIKEVPVTGPFVVDETRVRELDITPR
                     ELEILGLIATGLSNREIAGRLFVSENTVKTHSSRLFDKLGARRRTQAVQIGKTARLIP
                     "
     gene            9664..10074
                     /locus_tag="VGR03_00135"
     CDS             9664..10074
                     /locus_tag="VGR03_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1992719.1"
                     /translation="MKRVTGIGGIFFKSESPPQLYDWYEKHLGIQRESHGQGASFHWR
                     EPQAADGTEPGSKALTAWCIFPQTTKYFGASKASFMVNYRVDNLDALLEDLKKSGIEI
                     DPHREDYDYGRFAWITDPDGNRIELWEPPKPKED"
     gene            10157..10705
                     /locus_tag="VGR03_00140"
     CDS             10157..10705
                     /locus_tag="VGR03_00140"
                     /inference="COORDINATES: protein motif:HMM:NF025227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HEV1992720.1"
                     /translation="MKKTVLTFGLISGAISSLLMVATLPFAHRIGFNKALIVGYTTIV
                     LSFLLVFFGIRSYRDNVGNGQITFKKAFAVGISITLISCIFYVVTWEILYFNFMHDFM
                     DNYGAHTIEKLKASGASPAAIQVQVQQLKKFKEQYENPLFNSLMTFIEPFPIGLAITL
                     ISAAVLRKKAQSQPAQSPLPAS"
     gene            complement(10912..12732)
                     /locus_tag="VGR03_00145"
     CDS             complement(10912..12732)
                     /locus_tag="VGR03_00145"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV1992721.1"
                     /translation="MSKSRRHFLTQTSLGLIGAAVASSTSAQDLANDLAQEPTQLPPG
                     APPAFGTGPAVGPEVSPATFAEAEKLVQVNLTTADRAMAAETWRVNLASLYERRTGPR
                     TIALEPTLAPWSRYNSVLPGQPSGPERDQFIRTKSDPGPLPANDADIAFAPVTQLSQW
                     IEQRKLTSERLTQIYLRRIEQFDPQLRCIITLTRDLALAQAKKADEEIAAGKYRGPLH
                     GIPWGGKDLLDTAGIPTTYGAEPFRNRIPKDDAAVVKRLHAAGAVLLAKLSLGALALN
                     DIWFGGQTVNPWLLEEGASGSSAGPGAATAAGLVGFSIGSETGGSIIAPSMRCGVTGL
                     RPTYGRVPRTGAMTLCWSLDKLGPMTRTVEDALLVLQAISGPDAGDLSSVPSRLDFDS
                     NSSIKGLRVGYFPAWMKQAPATAVDRAGLATLPKLGMVPVEVSLPNWPYNSLNLILFA
                     EGAAAFEELTLSHGVDQLKVQSPDAWPNLFRQARFLSAVDFVQADRFRRKVAQEMASL
                     FSQVDLLLVPSLRDEMLTISNNTGHPSLTLRAGFVQVSEARSDWAPDPKNPLPKFSPP
                     RRVPHGITLIGRLFEEGTLGRVGIALERAFAVSNQRPPSF"
     gene            12959..13945
                     /gene="glpX"
                     /locus_tag="VGR03_00150"
     CDS             12959..13945
                     /gene="glpX"
                     /locus_tag="VGR03_00150"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEV1992722.1"
                     /translation="MEEQLSLDFLRVVENAAIAAARTMGYGDRHHADEVAVEAMRKTL
                     DSVEIDGTIVIGEGERDEAPMLYIGEKVGLATHAPKALFPQVDIAVDPLEGTNLCATG
                     AANSIAVLAASERGGLLHAPDLYMEKLVVGPSCKGAVNLDAPVADNLKAIAKRLDRDV
                     EDLVVIVLDRPRHEKLIEEIRKAGARIRLIGDGDLSAGISAAVIGTGVHAVMGTGGAP
                     EGVLTAAALRCLNGQILARLVVSKPEDAERLAKMGVKDLTRIYDTEDLAPGKKMIFAC
                     TGVTDGNLLKGVRFFGEGVRTSSMILTLDERQVRFIDSVHLEKRPDIKVRFN"
     gene            complement(14017..15141)
                     /locus_tag="VGR03_00155"
     CDS             complement(14017..15141)
                     /locus_tag="VGR03_00155"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEV1992723.1"
                     /translation="MKIPLSAPDITEADIQAVSAVLRTSRLSLGPQMEEFEQSIARYV
                     GTSHAIAVNSGTSALHLCIRALGISEGDEVIVPSFTFIAVANVLRYERAVPVFIDIDP
                     QTLNLDPARIEEAITPRTRAIIVVHTFGCPAALDEIMEIARRHKLFVLEDACEALGAQ
                     FDGRKVGSFGDAGVFGFYPNKQITSGEGGMIVSDNLKVTALARKLRNQGRSESGEWLQ
                     HEELGYNYRISELNCALGASQLKRLETILERREAIAREYHRRLESQPDLELPPIALPR
                     RRISWFVYVLSLRERFTASHRDHIVREMASRGIACGRYFAPIHLQPAYRSQPHRCMTL
                     AHTESIAPRTLALPFFNKISVEQIEEVCQTLGEVLRKLRG"
     gene            complement(15138..16328)
                     /gene="neuC"
                     /locus_tag="VGR03_00160"
     CDS             complement(15138..16328)
                     /gene="neuC"
                     /locus_tag="VGR03_00160"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEV1992724.1"
                     /translation="MKRKIAVITTSRADYGHLYWPLRDLSAHPLVDLKLIVLGSHLSP
                     EFGHTVREIEKDGFPIAAKIECLLSSDSDVAMAKTIGLATLSLADCLGQMRPEILLLI
                     ADRYEMLAPASVALALRIPIAHIEGGEISEGAIDDAVRNALTKMSHIHFTSTCEARRR
                     IIAMDEEEWRVHRAGAPSLDHLRRRTLHTHEELESMLKLKLEKPSILVAYHPVTIARD
                     TLQEVDALSEALASLPEQILFCYPNADTGSRDLIARARSFAASRRNSHVFVNLDALTY
                     WSLLRQVDVFIGNSSSGIMETPSLALPTVNVGLRQQGRERARNIIDAAPNAHAILDAL
                     RTAKSPAFRESLRGMTNPYGDGFASEKIVQVLTTVPISQELLMKRHSFLPAFEPANPS
                     TRPQ"
     gene            complement(16336..17418)
                     /locus_tag="VGR03_00165"
     CDS             complement(16336..17418)
                     /locus_tag="VGR03_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1992725.1"
                     /translation="MNAPLLGTTNTGKTILVTGAGGCIGSALVQALASSNPRFLVLLD
                     HSEQNLHEINLQLAAAGKSSYAAILGDILDGPLMTEIFDRYHPQTIYHAAAFKHVPLM
                     ESNPIAVVRNNVLGTWELARAAARFGAERLLMISTDKAVNPRSIMGASKRVAELALLL
                     LSDSTTANAKTSIATTKMNAVRLGNVLGSHGSVGPLFQRQIERGGPVTVTHPEASRYF
                     LMLSETVELIMAAAAVDDSGSILIPKMCEPVKILDFATRMIREAGLESPRDIEIVLAG
                     LRPGDKLEEELVYADEALQPSSDERLYRANGPRCDPQVLDASLQNISESVRERNLTAL
                     IAALGKLVPEYKPSETLLALLNPSLA"
     gene            complement(17481..19004)
                     /locus_tag="VGR03_00170"
     CDS             complement(17481..19004)
                     /locus_tag="VGR03_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992726.1"
                     /translation="MKRSWTALAVALVAILAVAGCNDYGNTFQNNTGASIAFLSPAQI
                     SACSANCADFTLTVNGRGFVAKTVVQWNGKSLPTALTLDSGGNIVNVTATVPAALVAK
                     PGVATVITLNPAYGAGQNGLSNPIAFIINPPRNPVPVLTSTSLSPNNLTSCGSACGSG
                     SFTLNISGTQFLSSSDPTQQSQVQWTFGSTQMTIAPSTVTSTSITVSIPNSFLATAGA
                     ATVSVRNPPAPSSGGGGGSSNGEPFNVCSNSQPCPPPSQASASSAAVAEETPAVSLDG
                     RYVAFTGLENDRAQIFLRDTCEGIPSGCQPRTSLLSVAADGSAANEDSHAPTISADGR
                     YVAFSSAATNLLENSPAGRQIYLRDTCAGAGDSCKPSTTLVSVDSAGTLVGTESILPS
                     ISSSGRFIAFLAITPSHSSNQVSAQLKTPASGNDSGYRQVFIRDTCLGAANCTPKTTR
                     ILLQPGDGTGSGQKSAGPALSGNARHVAIVAGNAATLFTRSVAVDDRVFLAIANQQH"
     gene            complement(19340..20293)
                     /locus_tag="VGR03_00175"
     CDS             complement(19340..20293)
                     /locus_tag="VGR03_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992727.1"
                     /translation="MNASRIARCAGLACLLLLAPLAITARASNLTLPPETPAILDKIY
                     SFDLEGAIEAAKRMEQERPNHPLGYLLEAEALWWRIWCTSSDFKYGMSDARRRPKLEA
                     DRHYFQLAARALSLAEAQIKHSESAEMQFYAGMAEASSARLYGLRVENRNAARSGVRA
                     REHLLRAKALDADLADADLGLGLYNYYVDTLSAIARILRFFMGIPSGSKQDGVRLLQQ
                     AIAHGVLTSNVARFYLALNLHRYDQQYEKALNILGPLVEKYPANPLFQLARGDLYAKL
                     GRKQQALACYRAANALPVQNRECQTRVQELVRASIAALGAQ"
     gene            complement(20325..22910)
                     /locus_tag="VGR03_00180"
     CDS             complement(20325..22910)
                     /locus_tag="VGR03_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV1992728.1"
                     /translation="MSSSASPIPQPRFSVSPRKVKAALAGLFSLTALLWLGSRTLLGA
                     NFLPHWYCYLGNLRLLWSNVIADAVIGLSYVAISATLVWLVRRAGRDLPYPYFFWAFG
                     VFIVSCGATHFTEVVTVWKPVYWLLAAVKIVTATASAGTAVVLIVAANDIVEFVRTAR
                     EAAARRGNEQFRALVNASPMAVVSADLEGKVTVWNPAAKRLFGWSAVEIVGSTVPFTP
                     PEREEERNELREEIMAGEIVKGFETLRLNRAGERIPVSISAAPMFDENGKLVSTMHVI
                     EDISERKRIEIELQEKTDLLSTVTHALNTYLDVGDWTAASRHLLAFAIQKTQSEYGFL
                     GVVLEGPVLRILAHDGIVWDTKLNRELFEGKLNQFATCGYFEVEHKHNLLGEIILKGE
                     TIITNSPQSDPRAGGVPAGHPAMHSLAGVPIFTGKETVGLIAVANRPGGYTGQQVRYL
                     EAMSQATGILYDNYRQSLKRTALEDEQKRLESQVRQAQKMEVLGRLAGGVAHDFNNML
                     MVLGGCTELLDRSLSRESPARIYLDQIQRTTEKAAAITKQLLTFSRKQVLEIHPMDLH
                     ESLTESECMLPRLLGSDIELTFHHDAAKSWILSAPTQIEQVIANLAINARDAMPEGGR
                     LAISTRNATILPEEGADSPSLSGNWVVLEVSDTGSGIDENTQAQIFEPFFTTKPAGRG
                     TGLGLATVYGIVKQSNGHIRVVSTPGKGARFQLYFPLVETAVPESQVSSPAENTEDAG
                     GGATILVVDDEAALRHAVVEILRASGYRVFEAQTVPDTLEIAEQHVGELDVLLTDIVM
                     PVLRGPELARRVAKIHPDIRVVYMSGYAEGFSEAELPANSVFLQKPFRFATLLEQLKL
                     VRRRV"
     gene            23213..24580
                     /locus_tag="VGR03_00185"
     CDS             23213..24580
                     /locus_tag="VGR03_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV1992729.1"
                     /translation="MGNKTENTPLERGLGLKEAVALNMIEIVGIGPFVVSSLVIRAMG
                     GPQALIAWLAGALLATLDAFVWSELGAAMPKAGGTYVFLREAYGPERWGRLMSFLFVW
                     QTLVQAPLSVASASIGFAKYAGYLYPFTPLQAKAISGGLVFFIVILLYRRITAIGKIS
                     VLLWIGVVGTMLWLIWGGLRHFDAKIAFDFPPGAFDLSWVWFAGLGSAMVSTIYSYWG
                     YYNICHLGSEIKDPERNIPRGIFLSVLGITILYLAMQTSLLGVVPWREAQNSDFIASL
                     FVERLYGQAAARFVTGMILWIAVASAFSVLLGASRVPYSAAQDGNFFPVFGKVHPTKH
                     FPHVSLLVLAGLAFIFSISLKLKQAIAGILAMRLLVQFIGQAVGVILLRRRRGTQGLP
                     FKMWLYPLPAVLTMFGWAWLFWQTGPTRKWGLAEIALGTLAYLVRAREVRQWPFGSTP
                     PAVPS"
     gene            24618..27251
                     /locus_tag="VGR03_00190"
     CDS             24618..27251
                     /locus_tag="VGR03_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV1992730.1"
                     /translation="MNFSFQRLAIVVLLGSGMTAILQTPVAAQFPPAPGTGPGLAETI
                     EAIDSARVTTRILYITAHPDDESAATLTYLERGLHAEVALLSLTRGEGGQNDLGPEQA
                     PQLGLIRTQELLAATHGYGVKLFFTRASDFGFSKTPEETEKVWGDQVLEDMVRVIRTF
                     QPHIVINGWGGVHGGHGHHQAAGLLTPKAVQLAADPAYKLRESTAGETLSPWGDRRPM
                     VLLDLDRSETPTGYMLPLDEVSPLYGKSWREIGLDAFANHRTQGIAGFLGSPFLRRPI
                     ALKQEDGGAFDPAVLAEPLGPLDEDYEVGNKSVDPLMRAVDQSLVAAREAALRLDWKA
                     AAASLAGAGRKISEVPTPSDAAQVPAPVESLGRSIKKKREKIDIALALVAGLRLDANA
                     DRSELVPGETFSIRVDAHHRQGITGEFKKTGLLLPPDWSVVKEESEAGGAIRWTIAVA
                     QNAQTASDGSGRPSNSRNAFANLLPEPPPLVTAMQEAVLDGYAFTVSSPVTNAHATST
                     RVDRIAPRIVPAYTLAVEPKHVVEVLAKQRKPFDVLLRVHSYATQPGKVSASVDVPRG
                     WKASAAVPLKFSGAGDQYARLTVTPPIKLVAGNYKIAAHAIRSADRAAGGKAERYGTS
                     LEPLPTLPTLLWSEPAQCAVHSFAVSVPPNLRVGYITAESEPIPEALQRLGIHVETLD
                     ARALAFSDLSKFGAIVVGVRAYELRPELPGANQRLLDYVSKGGTLVVQYQREFVWDKT
                     QFAPFPALISPPIPLVKDGAPQTPRPLPRITDENSPVKFLKPHDPLLNTPNKITQDDF
                     RGWVQERGLYFWTQFDPKYTPLLAMNDPGESDLNGALVYTRYGKGTYIYTGLAFFRQL
                     PEGVPGAYRLFVNLLSASRSR"
     gene            27386..28003
                     /locus_tag="VGR03_00195"
     CDS             27386..28003
                     /locus_tag="VGR03_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1992731.1"
                     /translation="MSNHHGTRFAGRTVHQVILLLLATALSLTCVSPAAAQKKKKSDP
                     PPSTDRSKMLVSLTDEQQIDYTLSEMLGAWQLGDIEKLRSDYGDDVAIVNGSWAPPIL
                     GWTNYLAVYQQQRARMQQVRMDRTNTYIKVSGTVGWACYQWDFEAVVDGQQMASQGQT
                     TVVLEKRNNHWVIVLNHTSIPPRIPQAAPANAPSGQQQPAKPTPR"
     gene            complement(28037..28327)
                     /locus_tag="VGR03_00200"
     CDS             complement(28037..28327)
                     /locus_tag="VGR03_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992732.1"
                     /translation="MESQTLTGPLLYMLIAWGIVTAAFLVLLLRRSLLASHEDDQIFL
                     DAAEEHMAREQRELVAKINALSRPIMGTGILAGVLLLVIAGMWLKEGLKGLF"
     gene            28759..28845
                     /locus_tag="VGR03_00205"
     tRNA            28759..28845
                     /locus_tag="VGR03_00205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28793..28795,aa:Leu,seq:cag)
     gene            28950..29732
                     /locus_tag="VGR03_00210"
     CDS             28950..29732
                     /locus_tag="VGR03_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992733.1"
                     /translation="MVFLLLALSFPEWRPHFSFLDTPHANEMLALRWLHLVFGIIWIG
                     LLYFFNLVLTPAMKKCDPKLRIKIYPELMPGAMEWFRWSALVTVVVGIRYFTIHLASD
                     AKLAGDRSLTGTWFGWWLLVWLAAYVFIYALQLPAKGIFNSPWFRVIGVSLVVITASW
                     LVLALNGGPNVSNAHLAISVGGGLGFIMLLNTWGVIWRVQKRLIGWARTSAEHGAPMP
                     PEAERLMRWNYLTARTSFWLSFPMLFFMGAASHYPFLSSIAR"
     gene            29751..30983
                     /locus_tag="VGR03_00215"
     CDS             29751..30983
                     /locus_tag="VGR03_00215"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEV1992734.1"
                     /translation="MSDEKNYNVLTAETGVPIKAWTKGVQLEDQARKQLLNVAQLPFI
                     FKWVAAMPDVHWGVGATVGSVIPTKGAIIPAAVGVDIGCGMMAVQTDLNARDLPDNLK
                     GIRSAIEQAVPHGRTNHGGHGDVGAWREIPSRNNEVWQTLKPRYDAILEKHPKLDRGN
                     HSNHLGTLGTGNHFIEVCLDEAENVWFMLHSGSRGVGNRMGSYFIEVARKDMQKFFIN
                     LPDKDLAYFSEGTEHFDDYVEAVEWAQDFARFNRDLMMEQIVGVVRNSGEVRPFIAGL
                     KAINCHHNYVARERHYGQNILVTRKGAVRAREGDMGIIPGSMGARSYIVRGKGNLESF
                     MSCSHGAGRAMSRTEAKRRFTVEDHKRMTAGVECRKDADVIDETPAAYKPIEAVMAAQ
                     SDLVEIVHTLRQVVCVKG"
     gene            31149..33725
                     /locus_tag="VGR03_00220"
     CDS             31149..33725
                     /locus_tag="VGR03_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEV1992735.1"
                     /translation="MTKITVEISACRLARKPMPLPSHLSPDNILRFLQVRSEPASTSE
                     IAQGLHLKKSDNRPLFKMLSKLKKRRAIEELLGGRYRLPGRKSEREAATQKLSQDAVR
                     HPQHSALADRDEVKGRLVLHHDGYGFVVPESPMPHLDGDIFIPRDAIQDAMHGDHVLA
                     KIQRLGGVTGAQRAEGRIIRILGRAHPTVVGLFRYGTQQNVVLPYDVRIQHEVEIPRG
                     NELTPGLWKKLGFSGADETSIRSRRIPRMDELDGAVVNVELLRYPQGGAAATGRVIEI
                     LGRPGDLGVDIEIIIRKHHLPHSFSAEVLDEAEHGAKPVSETQRADREDFRHLPIVTI
                     DGETARDFDDAIHVEHRDDGGWHLQVHIADVAHYVRTGSALDREARLRGTSVYFPDRA
                     VPMLPEALSNGMCSLKPQEDRLVMSALMEFNAAGHMNSSRMTSGVIRSAERMTYTNVN
                     RVLEGDAEMTERYAVLAPHFREMKELALLLNARRNEHSSIDFDLPEPVIEFDEEQRMT
                     NITRSERNIAHRLIEEFMLAANRAVDTYLLQRSIESLHRVHEKPDARKVLEFEELARA
                     FGYSLGVEDLHQREVAVRHGRVPAPAKAGRPDSYGHGRERGMKVALPGGELRITPQHY
                     QRLIRKVVGKPEERIISYLMLRSLKQARYAAEPLGHFALGFDEYTHFTSPIRRYPDLI
                     VHRILKWALDHPKDAPASSKTHVAASLGSESTLYSHGNLEEIASETSEAERRANGAER
                     ELMDWKTAQFMEEHIGEEYDGLIISVQKYGCFVELFEVFVEGLLPVGALEEFAGTRCV
                     LRERDHAIVALPGGGSRGAAGARRGRGAKPRQLEWHHGDKVRVRAERIDPMRKRVEFA
                     LV"
     gene            complement(33753..34301)
                     /locus_tag="VGR03_00225"
     CDS             complement(33753..34301)
                     /locus_tag="VGR03_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEV1992736.1"
                     /translation="MENRKDQGKQTNRISGERRADDALRIADALKRRGRISLRVHGTS
                     MQPWVRPKDIALIRQISIENVRCGDVVLFRREHHLFVHRIVEKRGSLNAAQLFSKGDA
                     HPTSDGVVQEQELLGRVMRIYRNGRRIDLDAPRQLALGLFISQLSRHSRFWYPLAKFA
                     AAVTRPVRRVMNALHISSAAVR"
     gene            complement(34599..34760)
                     /locus_tag="VGR03_00230"
     CDS             complement(34599..34760)
                     /locus_tag="VGR03_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992737.1"
                     /translation="MAEENKNSPPAPGEKKPYTKPSVVSEPIYETLALACGKLPGQGG
                     VCNGAPRRS"
     gene            complement(34772..35980)
                     /locus_tag="VGR03_00235"
     CDS             complement(34772..35980)
                     /locus_tag="VGR03_00235"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV1992738.1"
                     /translation="MTATLAASATPVMDRLISRTVEKHRPLSVHFDLTYRCNERCVHC
                     YLDHDDHGEMTTAECLKVLDDLARSGALFLTFSGGEIFLRPDLYEILAAARRLHFDIS
                     LKSNALLVTPERAARLREFGVRRVQISVYSDIPAVHDAITKVPGSLQRTLAAIPILLE
                     HGMQVKLACPLMQENLMAYRGVMALAEKLGVPYILDLTITPMIDGSSGPLAHRASVSS
                     LLPVLRDPALHACKPQPSAQAASAMNALPPSFGSAVSSGIESLAYEDLPCSAGHNSCY
                     ISPYGDVFPCVQLPQAAGNLRREKFEDIWYHAPQLERLRAIRESQLPICSRCEIRSYC
                     ERCPGLALMEGGDLLGAYERACALAEEKARLAGVAAPISAFHVEQNTTLVQTTPEAEY
                     ASLATDSGSD"
     gene            complement(35977..36279)
                     /locus_tag="VGR03_00240"
     CDS             complement(35977..36279)
                     /locus_tag="VGR03_00240"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEV1992739.1"
                     /translation="MSTPASVSWQKNPALAWREIDDETIIISPNDSVMHELNDTGSFL
                     WKNIDGKKSAAELAELLAENYEVTPDIALSDTQALLEQMSSRKLVVTVPATGGVSP"
     gene            complement(36276..>36720)
                     /locus_tag="VGR03_00245"
     CDS             complement(36276..>36720)
                     /locus_tag="VGR03_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992740.1"
                     /translation="FLLHAATVVRNGKAYIFAGRSGAGKSTVASLSPEGSVLTDEISL
                     LRREDGAWRAYGTPFWGEFRAAGSNTSVPVAGIFRLLQAAENRVETLRPVAILRALLP
                     NVLFFSAEVEANRQLLEILNQAVTEIAGYNLAFRKNSTFWEVLPA"
BASE COUNT         7648 a        10116 c        11417 g         7539 t
ORIGIN      
        1 aatactctcg gccgccgccc gcagcggccc ccagcggagc tttcttcagc atatggtaat
       61 ggcccgccgg cggactcgcc agcgccagaa ttccaagcag cacaaaagac aggcaacacg
      121 caaaacggcc cagaaccttg atcgacatgg aaatcctccg ttacgtcatg gattacggaa
      181 ttgtcggcat caacgcgaat cgtggcatgc cttcgcggct ggccaatatt ttctgttgtg
      241 tgcggattgt acctcaattc tggagtttgc ggctcggtga cgcagaaatg cccgaggtct
      301 cttacctcaa atcgcaaatc gcattctttt attgcttcgt ctgggaagcc gtttggaccg
      361 ggatgtcgta gacgtagcag atgacgagtg gcgcgttgct gcggccagtg gtcatgtgca
      421 gcgtttcgta gagttgccgg ccgtcataag agagttcaaa tgtgcggctc atcttgttgc
      481 cccgcggatc tttttcttcg gtgcccaggc ggttgccgtc ccacttggcg gcgatctcct
      541 gatagttttc atctttggat ttctgtaatt tccgtccgtc ggtcatgaac gcccgtttgc
      601 gatcccggtc atccaccagg tcgacttccg cgcccgtcat cgacagcgta atcgcgcgcg
      661 ccggggtgaa gagttcgtgc attttctgcc gctcctcatc gctctctccg ccgcgcccac
      721 cgtagctgcc gtgaccaccg ccgggatacc cgccacccat gcggccatgt ccgccaccgt
      781 ggccaccgcc attcgagcct cgggagtctt gcctcctctt acttgcctcg tcgctgtcgt
      841 cgggattgaa cttccaggcg ccaagaattg ttgtgcgcgg cgcgggcgcc ggattcggag
      901 cattcgccac agttgcggga gcgggctgcg acggcgccgc cgttataggc cccgatggag
      961 cttgcgcgtg aattgcaccg ggaacaatgc agctgagtcc aacgagaatc gttagaaatg
     1021 gcagcctggc aaattggatg acttgcatgg ctaaatccaa aattctttgc agaaccccat
     1081 gttgtattcc catctcggaa tgcaaaagtt cccgccagat tacttaagcc atgccttcag
     1141 gtctgtggca cgtggaagaa gagcgagggc tccgagttcg cggaaccgcg cggcacgctg
     1201 gcgataaccg tgttcgcccg gagcgatgat ggccatgaag ggaacgctgg cgtcgcgcgc
     1261 ggcgagcgcg tcgtcaatgt tgtcgcccac atagagagcc gtcgacggat cgcgcccttc
     1321 aagaattttg cgcaatcctt ccggatgcgg cttcctcttt gcatcgtcca tcgtgatgat
     1381 cgtgcgaaag aattttgtag caggccattt ctcaaacgta aaggaaaatt cctggcgcgt
     1441 gcgcccggtg aagagattca gctcgaagct ctgcgcccat ttttcaatct gccgaggcgt
     1501 gacgatcagt ttttcgtttc gaacgtttcc cggcttccca tcgctgcccc agtagaattt
     1561 ctcgaaggcc gcgcgcgctt ctgcgtacga agtgggatgc ccgagcgaac ttacccacgc
     1621 ggacaccatg ttccaatcat cattgaagcc cggcttgctt ttccatttgt gcagttcggc
     1681 gtacgtgacg cgtttgcccg tcaggtagcg caccgtatcc agcgccgagc gccagtaagt
     1741 gccgcgcaca tcgacgagca ctccatcgac atcaaaaatc agaatcctgg gtggcgttgt
     1801 gagcagcttg cggcgtggcg cccgttttgt ggacgccaaa gagtttttcg gcacggatcc
     1861 tcgttctgaa tatgtttctg cgacaaaaca agccggaaag aactccggct gcaagacaaa
     1921 agtgtcgctg ctcgccacgc ctcgcgtcaa gcgcgccacg gttgctggcg aatccggcgc
     1981 gttgctacac tggacgggca cgctgcgcaa ggagccgaga atggccaagt atggcggagt
     2041 ggattttatt gattttgatt cgcaattgaa cgacgaagaa aaactggtgc ggcagacggc
     2101 gcgcgaattc gtcgaaaacg aaattattcc catcatcgaa aaacacagcc gcgagggcgt
     2161 tttcccgaag catctcgtgc cgcagctcgg cgagctggga tttttcggag cgaacctgca
     2221 cggatacggc tgcgcgggaa tgtccaacgt cgcatacggt ttgatgacgc aggaattgga
     2281 gcgcggggat tccgggttgc gcagttttgt ttcggtgcag ggcgcgctgg tgatgtatcc
     2341 gatttattcg tatgggagcg acgcgcagaa ggacaaatgg ctgccgctct tgcagcaggg
     2401 caaagccatc ggctgcttcg gcttgacgga accgcaattc ggatcgaatc cgggcgggat
     2461 gctcgcgcgc gcggtaaaga agggcaacaa gtacattttg aatggcgaaa agatgtggat
     2521 tacgagcgga tcgatcgcgg acgtggcggt ggtgtgggcg aaaaccgagg acgacaaagt
     2581 tcgtggattt ctggtggaaa aaggcgcgcc gggatttaag gcctgggaca ttcacggaaa
     2641 gtggtcgctg cgcgcttcgg tgacttcagg cctggcgttc acggattgcg agatcccgga
     2701 agaaaatctt ttgcccggcg tggtcggctt gaaagggccg ctgagttgcc tgaatcaggc
     2761 gcgttatgga atcggatggg gcgcgctggg agcggcgatg gcgtgctatg acacggcgct
     2821 gcagtatgcg aagacgcgca agcagtttgc gaacaagccg atcgcttcgc accaactggt
     2881 gcaagaaaag ctggcgtgga tgatcacgga aatcgtgaag gggcaattgc tggcgctgca
     2941 cgtcggccga ctgaaggaca gcggcaaagt tgatccgtca cacatctcga tgctgaagat
     3001 gaacaacgtg gccatcgcgc tggatacagc gcgcaaagca cgggacattc tcggtgcaaa
     3061 cggcatcgtg gacgattact gcatcatgcg gcacatgaac aatctcgaaa gcgtgttcac
     3121 ctacgaggga acgaacgaca ttcacaagct ggtgatcggc gaacgcatca ccggaattgc
     3181 cgcgtacgtg tagagaccga aagggcggac tgaagtccgc ccctacattt cccagccaac
     3241 ccatctccag ctaggccgaa tcttgtatgc taatggggcg cagcgtgctg cgcccctaca
     3301 ggagtaaact cccatgcgct cgcgtgcgac aaccttgact cgattcctcc gctattcgct
     3361 tgtaacgctg ctcgtaaccc tagctggcaa ctgcgaaatt gtttgcgcgc agcagaaaag
     3421 tgcggcggcg caggagaagc cgaagatacg ggcgatcacg gccttcatca atctggatcg
     3481 ggcgcagtac aaggagcagg tggcggatgc gctgaaaatg ctgaagcgcg cgcggacgat
     3541 ttttgaatcg cgcggatatc aggtgcaaac gatccggatc gcgacgcaac cgtttccgga
     3601 gtacaccaag gggctgacga aggaacagac gattgcgttt ttcaaggagt acgacgcgct
     3661 ggccgtgaag gaaaagttcg cggcctcgat cgggccggcg atgctgaatg ccgggggcag
     3721 cgattcgcaa gcggagctgc ttgcggaaat cctcagcaac acgaaaacgc tgaatgccag
     3781 cctggtggtt gcgggcgcgg acggcgtgcg ctggggcgcg gtgggcgccg cggctcgcgt
     3841 aatgaagaaa ctcgaggaga cggaacatag ccagggcaat tttcgttttg cggcgattgc
     3901 gatggtgccg ccgctgacgc cgttttttcc ggcggcgtat caaaccggct tcgggcatca
     3961 attcgcgatc gcgctggaat cggcgaacgt ggtggctgcg gcgttcaaag atgcgccgga
     4021 cctggcgacg gccaagcagc gtctgacaga tttacttgcg agctcggctt tcgacatcga
     4081 gcggcacgcg ggacgaatcg accaggagac cggctgggcg tatatgggaa tcgatctttc
     4141 acccgcgcca tcgggagatg tttccatcgg tgcggccatc gaaaatctga ccacgcagcc
     4201 catcggcatg agcggaacgc tgacggcggc agcgacgatt acagcggccg tgaaagacgt
     4261 cagagtaaaa cagatcggat attccggctt gatgcttccc atccttgaag atacgcggct
     4321 ggcgcagcgc tggagcgaag gacacatttc catcgatgcg ctgctcagtt attcggcggt
     4381 gtgcggtacg ggcctggaca ccgtaccgct gcccggcgac gtcagcgcgg agcagctttc
     4441 gctcattatc ggagacatgg ccagcctggc cttcaagtgg cataagcccc tttcggcacg
     4501 gttgctccct gttctgggca agggctgggg tgaaatgacg gagtttgatg atccgttcct
     4561 ggtgaatgcg aagctgcagt cgctggacat caagtgacgt tgtcagtcgt cagtcttcag
     4621 ttcgcagtgg gagaagaaga atcactctct tctctcgggg ccgattcctc ggggcacctt
     4681 gaggtgccgc ggcctaggac gtgaggagag atccctcggc tcctgatggt cgctcgggat
     4741 agacatgctt tgtcgcatgt ctagttaacc ataacgaccc caccccctat gttttttgta
     4801 agtgttgcat ctaaaggact tgagttccgt gtaagtgatt tagaatcaac acttgcgggc
     4861 acttctataa gtgttgattc taaaggtagt tagagctgtc tttttggacc atttctcgtg
     4921 caggtcggga tcccctactc ccccatcaat gatggatgaa tatcaaaaca aaaaacttag
     4981 gaaatcttca tttcataaac gacagattat aaaggacttc gatgacggta aatgcgggcc
     5041 gccaaaacgc ttgcggctta tcccctatat ccccgttgtg acgtaaagag tcgggattat
     5101 atgcctgacg cacccgtaga gagggcgtaa gttatgaagc cccgtgacgg gatagcaaga
     5161 agtagaggta ttctttcccc taaagtcgga gggggtttgt cagaatatgc tcgtagaaat
     5221 attagaaaaa ctaaaacagg aatggcgcgt gattaagggc gcgccattcg cttttacgtt
     5281 tgtttgcgtc gttgctgtta gcgcttgctt ggggggcttc cgatggttct atcatgaaaa
     5341 gctgaccgag agtgaaacac tcgccaacca ttggaaatca gaagcagatt actggagaga
     5401 tgtggcgtcg catccctcgc agacttcccc gcccgcagca acacagccag ttcaagggaa
     5461 gccaattccc ccaaagatcg taacgcgcac tttgccagcg ccagaatttg gaacgcaagt
     5521 cgttcatcgc gagaatttta ttcccggtga ttctaagtat ggctctgggc ttagattgac
     5581 ccttgcaacc gatcagagaa gggggccagt gcaactgctc atcatttgcg atggcgacat
     5641 aggacttgct cctagtgcag ggaaatcatc aaagagtggc gtattcgtcg tagagtcgca
     5701 aatgctaatg ccgggtcatc ccgaggtttg gaatgtcaaa tggcgcaccc cggaatggac
     5761 accgggcgac accataatgt ttgaattttt gtccaagaag aaaatagacg ccaacgcggt
     5821 tattcctatc acttacaacc caaacgcgca gtaggcagtt aggctttacc cctgcgtttc
     5881 ttgttccgtt tctttggctc cggcttgacc gtgagaaatg cggaaaccgc agattgaaac
     5941 gcgaacggga ttttcagctt gcggttgcaa ttctttggca ttgtcctcat ggttctattg
     6001 gtgtcgtctt gcagacggac agtaagataa ctcggtcatc atcaaaagtg taataaatcg
     6061 caagatcaac ggtccaccct gcaatgaacc aaacctgcgt attgccgata cgatggccac
     6121 attccggcag tcttgccaga accatctcca cgccttccaa aaacgcgtct gccctctcta
     6181 tatgcggttc aagagcctgc aattcctctt caaaccgact gttcttgacg atacttctta
     6241 gcttatccat tggcgcgggt caagcaatcc aacgccagcg gcgctagttc aagcccgtat
     6301 gcctgttcct ctgtaatggg cggcctcgaa ccaactaagg ctgtgctgta agggatggtc
     6361 tccttgtttc tggctacttt ccatcccaga aacaggtgag atttctcgct tatttcagat
     6421 gctccaagat tccaatattt ttgaatcatt gtatggacta ggtctatttc ctggggggtg
     6481 aacacactga catcaggctg tcgaagagca attggcttct tctgggtatg accaaaatat
     6541 tgaatttcct cgtaggccag ctctccgctt tgctgcattt tctccacgac cgggagtaac
     6601 ttccttggag caggcccctt tggcaatttg aagtattctt gtcccgtgat ggcggtacca
     6661 aacgtcaagt aagctgaaaa gtcaccataa aaaagcaact tattcaactt tatggacccg
     6721 aatttcttgt cgccctgaga acgggcacaa atgagcagga taagctctct gaatttcttt
     6781 tcggatgttc tggccatgac cgtaactcct tgcttagtat aggcgaataa aaagcgaact
     6841 acaaactgct ttccgccgta cgggtcactt atacattaga cgcttcccgg tagtttcttg
     6901 catggccaaa gagggaccgc tcatcgtcat ttgcatgacg cgagtcatcc cgaaaatcga
     6961 gttggctgac acatcgacgc aagagctgct tccctacacg gtggtataca ccgtttacgc
     7021 tcccattgag aatcgaaaag tagccttcaa tcgcatttgt gctgattagg acatctccct
     7081 ctttacgagg tccaagtgtg gcgaaccgca ctctgaagac gggaatggtt aatgatttag
     7141 ctatagccag catgatccgg ataaggttga gagaagagag gattgcttaa ttctcagcgc
     7201 tgaaaaagat aatgcgggac gaggccacca cgcagcagca cgcaggatta tccacgtcgg
     7261 ataatacctc gacggttaac ggaagtcaag agatgagtgt gggtggtagc gcccccggcg
     7321 ccggtatcgg caacttcatt cttaaatcga ccagccttgg cggtattctc ggcgcaaata
     7381 gtcgttggct tggaggacgt tggtgatgcg catgttggcg ccgtcgtagt agatcttgcg
     7441 cccggcgcgc agcgccacga tgccgaggag catgacctcg gtgagacggg cggcgtactc
     7501 gaacgggcag gaggcttcca cctttccctt ggcgacgtcc acccagttca tttcgtgggc
     7561 ttcgccggcg atgcgaggga gcttccgcgg gggcttgccg aaggaatcgt ggagcgactt
     7621 gggcaagagc cggggcttga agccgtaggt gtcgtgaagg agcttgccct tgctgccgat
     7681 gagcagcgcg ccgccttctt tattcaattc ttcatcgccg agctcttcgg gcttattggg
     7741 cttgaggccg ccgtcgtacc aagtcagctt cacgggcggc atcggatcgc gcgcggggaa
     7801 gtcgtagaaa gtcagtgtgg cgtgcgggta agagacgccg ttgaaagggg tcgagacggt
     7861 ctcaacgctg gtgggaaagc ccaggttgag cgcccacatg gtgtggtcga tcaggtgagc
     7921 gcccatgtcg ccgatcgcgc cgacgcccca atcggtccag ccgcgccagt tgaaggggtg
     7981 atagatgggg tggtagtcca cattcggggc ggcgccaaga aaaagacccc attcgagccc
     8041 gccagggacg ggatagtcgc cacaaagggc ggcggcaagg cgcgtgttga cgtcgcggcc
     8101 gctccattta agaggttcgg gccccgcctt cagcggctcc gggcgcggca cgccttgggg
     8161 ccaaaagccg agagggcgat tggtccagat atgcacctcg cgcacgtcgc cgatggcgcc
     8221 gccccatatg tactcgacgg cggtgcgtcc atcgtccgag gagtgaccct ggttgcccat
     8281 ctgcgtggca actttcgtct ctcttgcgcg acgcgaaagc tttcgagctt cttcgaccga
     8341 ccagcaaaga ggtttctgga cgtaaacatg tttgccaagc tccatagccg ccaaagcgat
     8401 ggtggcgtgc atgtggtcgg gcgtggcgat cacgacggca tcgatgtcct tctggcgctc
     8461 aaacatctcg cgatagtcgg tgtagcgtgt ggctttcggg agatgagtgg acttcagctt
     8521 gatcatggcg tcgacccggg cctgggccct cacggcatca aacggcactg gcaccgcgcc
     8581 cggctgcggc ggggcgggca gctcgatgcg cttcttcagc cgctcgatat cgatgtcgag
     8641 gtggtcgaat cccttatcgg catagcgcca gtccacgtcg cagagcgcga cgatgttctg
     8701 gctggcgagg ttgatcagat tgctgcggcc catgccgccg actccaacgg cggcgatgtt
     8761 cagcgtgtcg ctgggaggag tgacgccacg gccgaggacg tggctaggaa caatggcgca
     8821 cgcggcgctt ccgacagcgg cgctggcgag gaatttacgc cgcgaaattt ctttcctttt
     8881 gctcatgggc atttatcctc ggctgcatta tcgcgcgctc gtggggattt tgcgtaggca
     8941 agttttgggc ttcgtcgaga tgaactcgtt acgaccaacc caacgactgg ttagacaagg
     9001 ctggcgctcc ccaagattgg agtcttcaga tatcatggcc tcccgtgacc ttgacgaggg
     9061 aatccgcccc gagttcgcgc cgccgcgcag tgcgcgacat cctgctctat ggcgtggccg
     9121 gcggcgtgct catcgcggtc ctgaagctga cggagtaccg cttcctggtc gtcgagcatt
     9181 ctgtggagat ttacggcgcg ctgatcgcgg ccctgtttgc cggattgggc atctggctcg
     9241 ggcagacact cacccgaaag aagccggcgg taatcatcaa ggaagtgccg gtgacggggc
     9301 cgttcgtcgt tgacgagacg cgcgtgagag aactcgacat taccccgcgc gaactggaga
     9361 ttctcgggct catcgccacc gggttgagca atcgagagat tgccggccgg ctattcgtca
     9421 gcgaaaacac ggtgaagacc cattcgagcc ggctatttga taagctcggc gcaagacggc
     9481 gcacgcaggc ggtgcagatc ggcaagaccg cccggctgat tccgtgaatc gtttgcctgc
     9541 ccggaggcgc cccacccgaa agggtgattt ttcgtcctgg aatgcaaaat cacccgaaag
     9601 ggtgacgaca acgtttacgt cctccgctac actcgggccc gtgaaatcag gtgaaggagg
     9661 ctcatgaagc gcgtaaccgg tattggtgga attttcttca aatcagagag cccgccgcag
     9721 ctctatgact ggtatgagaa gcatctcggc atccagcgcg aatcgcacgg ccagggagca
     9781 tcgtttcact ggcgcgaacc gcaggcagca gacggcaccg agcccggttc caaagccttg
     9841 actgcgtggt gcatcttccc acagacgacg aaatatttcg gcgcgagcaa agccagcttc
     9901 atggtcaact accgcgtcga caatctcgac gcgctgctcg aagacctgaa gaaatcggga
     9961 atcgaaatcg atccccaccg ggaagactac gactacggcc ggttcgcctg gatcaccgac
    10021 ccagacggca accgcattga gttatgggaa ccgccaaagc caaaggaaga ctgaccgcag
    10081 cgccggcggc ccccgctaaa tttcgggcac gttaaaccag gtttcacgga attgagggaa
    10141 aaggagactt aaatcgatga aaaagacggt gctgactttc gggctgattt cgggcgcaat
    10201 ttcctcgctg ctgatggtgg ccactttgcc gttcgcgcac aggataggct ttaacaaggc
    10261 cttgatcgtc ggttacacca ccatcgtgct ctcgttcttg ctggtgttct tcggcatccg
    10321 ctcgtaccgc gacaacgtcg gcaacggcca gatcacgttc aagaaagctt ttgccgtcgg
    10381 gatctccatt accctgattt cgtgcatctt ctatgtcgtg acctgggaaa ttctctactt
    10441 caacttcatg cacgacttca tggacaacta cggcgctcat acgatcgaga agctcaaggc
    10501 ctcgggcgcg agtcctgcag ccatccaggt gcaagttcag caactgaaga aattcaagga
    10561 gcagtacgaa aacccgctct tcaactcact gatgactttc atcgagccct tcccgattgg
    10621 gctcgccatc acgctgattt ccgcggcggt cctgaggaaa aaggcgcaat cgcaaccggc
    10681 acaatcgccc ttgccggcat cctaattgaa cccgttcccg cgaatgtgct ccttgcttct
    10741 cctagcgtcg tcgttgacgt tcatgagtaa ggcgcggata cggtgattct ggccgctttg
    10801 ccggctaatg ggaaactatc ccaatgcctt gcggcccgat ctctgacgtc ggcggtcatt
    10861 tggccaaacg ccggcaaagc ggagcaattc ctttcgcctg ctgcgggcgg gtcagaaaga
    10921 gggcgggcgc tggttggaga cggcgaaggc gcgctcgagg gcgatgccga cgcggccgag
    10981 ggtgccttcc tcgaagaggc ggccgatcag ggtgatgccg tggggcacgc ggcgcggggg
    11041 agaaaatttt ggcaagggat ttttcgggtc gggggcccag tccgaacgcg cttcggaaac
    11101 ttggacgaag ccggcgcgca gggtgagcga cgggtggccg gtgttgttgc tgatggtgag
    11161 catttcgtcg cgcagcgagg gcacgagcaa caagtccact tgcgagaaga ggctcgccat
    11221 ttcctgagct actttgcggc ggaagcgatc ggcttggacg aaatccacgg cggagaggaa
    11281 gcgggcctgg cggaaaaggt tgggccaggc gtcgggggac tgcactttaa gctggtccac
    11341 gccgtggctc agggtgagtt cctcgaaagc ggcggcgcct tcggcgaaga gaatcagatt
    11401 gagcgaatta tagggccaat tgggaagaga gacctcgacg ggaaccattc cgagcttcgg
    11461 aagagtggcc agcccggcgc ggtccacagc ggtggcggga gcttgtttca tccaagcagg
    11521 gaaatagccg acgcgcaggc ccttgatgct ggagttcgaa tcgaaatcga gccggctggg
    11581 tacgctggaa agatcgccgg catccggccc ggagatggct tgcaggacca gcaaggcatc
    11641 ctcaacggtg cgcgtcatgg ggccgagttt atcgagagac cagcagagtg tcatggcgcc
    11701 ggtgcgcggg acgcggccgt aggtggggcg cagtccggtg actccgcaac gcatgctggg
    11761 ggcgatgatg ctgccgccgg tctcgctgcc gatggagaag ccgacgaggc cggcagcggt
    11821 ggctgctcct ggaccggcgc tggaaccgga ggcaccttct tcgaggagcc acggattcac
    11881 ggtttgtccg ccgaaccaaa tatcgttgag ggcgagggcg ccgaggctga gctttgcaag
    11941 gagcacggca cctgcggcgt ggaggcgctt cacgacggcg gcgtcgtcct tgggaatgcg
    12001 gttgcggaaa ggctcggcgc cgtaggtggt tggaattccg gcggtgtcga gaagatcctt
    12061 gccgccccag ggaataccgt ggagggggcc gcggtatttg ccggcggcga tttcttcgtc
    12121 ggccttcttc gcttgcgcga gcgcgagatc gcgcgtgagg gtgatgatgc agcggagctg
    12181 gggatcgaat tgctcgatgc gccggaggta gatttgcgtg aggcgctcgg aggtgagttt
    12241 gcgctgctcg atccattgcg agagctgagt taccggggcg aaggcgatgt cggcatcgtt
    12301 ggcaggcagc gggcctgggt cggacttggt gcgaatgaat tgatcgcgct cggggccgga
    12361 gggctggcca gggagaacgg aattgtagcg cgaccagggt gcgagggtag gttcgagcgc
    12421 gatagtgcgg gggccggtgc ggcgctcgta gagcgaggct agatttacgc gccaggtctc
    12481 cgctgccatg gctcgatcgg cagttgttag attcacttgc acgagctttt ctgcttcggc
    12541 gaaggtcgcg ggcgacactt cggggccgac ggctgggccg gtaccgaatg cgggcggcgc
    12601 tccaggcggg agttgcgtgg gctcctgcgc gagatcgttt gcaaggtctt gcgcgctggt
    12661 gctcgaggcg acggctgcgc cgatgagacc aagcgaagtc tgggtcaaaa aatgcctgcg
    12721 cgatttggac ataggtcctc cgcgcagcat tgtagccgag tcttgcagtc agaaagtacg
    12781 aaggctcttc tcctttcgcg cgggcgggct ggctggcgaa tcgtaaatgg tcgacgggaa
    12841 actgttcttt ggcttatgat ggcgccgcgt tggcagggtg agaccaaaag aaaatcgccg
    12901 gcaaggatgc cggcgctacg acttcagaac tcgcgccgtc tcccgagagg atacgagtat
    12961 ggaagaacaa ctgagcctgg attttctgcg cgtggtggaa aatgccgcga tcgcggcggc
    13021 gcgcacgatg ggatacggcg accggcacca tgccgatgaa gttgccgtgg aagccatgcg
    13081 caagacgttg gactcggtgg agattgacgg gacgattgtg atcggtgaag gcgagcggga
    13141 tgaggcgccg atgttgtaca tcggtgaaaa ggtaggattg gcgacgcacg ctcccaaagc
    13201 attgtttccg caagtggaca tcgccgtgga tccgctggaa ggaacgaacc tctgcgcgac
    13261 gggcgcggcg aactcgatcg cggtgctggc ggcgagcgag cggggtggat tgctgcacgc
    13321 gcccgacctg tacatggaaa agctggttgt ggggccgtcg tgcaagggcg cggtgaacct
    13381 ggatgcaccg gtggccgaca acttgaaggc gattgccaag cggctggacc gcgacgtcga
    13441 ggatctggtg gtgatcgtgc tggaccggcc acggcacgaa aagctaattg aagagatacg
    13501 caaggcgggc gcgcgcatcc ggctgattgg cgatggcgac ctctcggccg gcatttccgc
    13561 cgcggtgatc gggacgggcg ttcacgcggt gatgggcacg ggcggcgcgc cggaaggcgt
    13621 gctaacggct gcggcgctac gctgtttgaa cgggcagatt cttgcgaggc tggtggtcag
    13681 caagccggag gacgcggagc gcctggcaaa aatgggcgtg aaggatctga cgcgcattta
    13741 cgacaccgaa gatcttgctc cagggaagaa aatgattttc gcatgcacgg gcgtgaccga
    13801 cggaaacttg ctgaagggcg tgcggttttt cggtgaaggc gtgcgcacaa gctccatgat
    13861 tctgacgctg gatgagaggc aagtgcggtt catcgattcg gtgcacctgg aaaagcggcc
    13921 ggacatcaag gtgcggttca actaagaatg cgttttcacg ttgaggtggt ggttgcgcag
    13981 gtgactggtg actcgtgact ggtgactggc ttaacgctac ccccgcagtt tgcggagcac
    14041 ttctcctagc gtctggcaga cctcttcgat ttgttccact gaaatcttat tgaaaaacgg
    14101 gagagcaagc gtgcgggggg cgatggactc ggtatgggca agggtcatgc agcgatgcgg
    14161 ctgcgagcga taggcgggct gcaagtgaat gggtgcgaaa taccttccgc aggcaatgcc
    14221 gcgcgacgcc atttcccgga caatgtggtc gcggtgagaa gcagtaaaac gttcacgcaa
    14281 gctaaggacg tagacgaacc aactgatgcg gcgacgtggt aacgctatgg ggggcaactc
    14341 caggtccggc tggctttcca gccggcggtg atattcgcgg gcgatggctt cgcgccgttc
    14401 aagaatcgtt tccaagcgct tgagctggct ggcgccaaga gcgcaattca attcggagat
    14461 gcgatagttg tagcccagct cttcgtgctg gagccattcg ccggattcgc tgcggccttg
    14521 attgcgaagt ttcctggcga gcgcggtgac cttcagattg tccgagacga tcatgccgcc
    14581 ttcgccggaa gtgatctgtt tattgggata aaacccgaag acacccgcgt ctccgaacga
    14641 tcctactttg cggccgtcga attgcgcgcc gagcgcttcg caagcgtcct cgaggacgaa
    14701 gagtttatga cgccgggcga tctccatgat ttcgtcgagc gcggcgggac atccgaaagt
    14761 gtgcacgacg atgatcgcgc gagtgcgtgg ggtgatggct tcttcgatcc gggcgggatc
    14821 gagattcaac gtctgaggat cgatatcaat gaagacgggc acggcgcgct cgtaacgaag
    14881 aacattggcg acggcgatga atgtgaaaga agggacgatg acttcatcgc cttcggagat
    14941 gcctagcgcg cgaatgcaga gatggagggc gctggttccg gaattcacgg cgatagcatg
    15001 cgaagtgcca acgtatcgcg cgatggactg ctcgaactct tccatttggg ggccgaggct
    15061 caaccgggac gtgcggagca ccgcgctgac agcctgaatg tcggcttcgg tgatatcggg
    15121 agcagagaga ggaattttca ttgtgggcga gtcgatgggt tggcaggttc gaaagcgggc
    15181 aagaatgagt ggcgcttcat caagagttcc tgggagatgg ggacggtggt caagacttgg
    15241 acgatctttt cggaggcgaa gccgtcgcca tagggattgg tcatgccgcg cagggattct
    15301 cgaaacgccg gacttttcgc ggtgcggagt gcatccagaa tcgcgtgagc gttcggagcg
    15361 gcgtcaataa tatttcgtgc gcgttcccga ccctgttggc gcaggccgac atttacggtg
    15421 ggcaaggcga gagaaggcgt ctccatgatg ccgctgctgg agtttccgat gaagacgtcc
    15481 acctggcgga gaaggctcca gtaggtaagg gcgtcgagat tgacgaacac gtgcgagttt
    15541 ctgcgcgatg cggcaaacga ccgcgcgcgc gcgatgagat cgcggctgcc agtgtcggca
    15601 ttgggatagc agaagagaat ttgttcgggg agggaggcga gcgcttcgga gagcgcatcc
    15661 acttcctgga gggtgtcgcg cgcaatagtc acaggatgat aggccacgag gatcgacggt
    15721 ttctcgagct tgagcttcag catggattcg agttcttcgt gcgtgtgcag agttctgcgg
    15781 cgcaagtggt ccagagaagg ggcacccgcg cgatggacgc gccactcctc ttcgtccatg
    15841 gcgatgatgc ggcgcctcgc ttcgcaggta gaagtgaagt gaatgtggct catcttcgtg
    15901 agggcgttgc gcacggcgtc atcgatcgct ccttcgctga tttcgccgcc ttcgatatgc
    15961 gcaatgggga ttcgcagggc cagggcgacg gaggcgggag caagcatctc gtagcgatcg
    16021 gcgatcaaga gaagaatttc cggacgcatc tggccgagac aatccgcgag gctcagcgtg
    16081 gccaagccga tggtcttggc catcgctaca tcggagtcgg aactcagcag acactcgatc
    16141 ttcgcagcga tcggaaagcc atccttttcg atttcacgga cggtgtggcc gaattcggga
    16201 gagagatgcg agcccaggac gatgagcttc aggtccacca gaggatgcgc ggaaagatcg
    16261 cggagcggcc agtaaagatg gccgtagtcg gcgcgggaag tggtgatgac ggcgattttg
    16321 cgtttcaagg ttttctcagg cgagcgaggg attcaagagc gcgaggagcg tttcgctggg
    16381 tttgtattcg ggaacgagct tgccgagcgc ggcgatgagg gcggttaggt ttcgctcccg
    16441 gacactttcc gaaatatttt gcagcgaagc gtccagaact tgcgggtcac accgggggcc
    16501 atttgcgcgg tagagccgct cgtcgctcga tggttggagc gcttcgtctg cgtaaacgag
    16561 ctcttcttcc agtttgtcgc ccgggcgcaa ccccgcaaga acaatctcga tgtcccgggg
    16621 actttcgaga ccggcttccc gaatcatgcg cgtggcaaaa tccaggattt tgacgggttc
    16681 gcacatcttg ggaatcagaa tgcttccgga atcatcaacg gcagcggcag ccataatcag
    16741 ttcgacggtt tcgctcagca tcaggaaata tctgctggcc tcggggtggg tcaccgtcac
    16801 gggtccgccg cgctcgattt gccgctgaaa cagggggcca acacttccgt gcgaacccaa
    16861 gacatttcca agccgcaccg cgttcatctt tgttgtggca atgcttgttt tcgcattggc
    16921 cgtagtcgaa tcgctcagga ggagcagggc cagctcggca acccgcttgg acgctcccat
    16981 gatgctgcgc ggattgacgg ctttgtcggt ggaaatcatc agcaggcgct ccgctccgaa
    17041 gcgggccgcc gccctggcca attcccacgt gccgaggaca ttgttgcgaa ccacggcgat
    17101 gggattcgat tccatcaggg gaacatgctt gaacgcggca gcgtgataga tcgtctgagg
    17161 atgatagcgg tcaaaaatct ccgtcatcaa cgggccgtcc agaatatctc cgagaatggc
    17221 cgcgtacgag gatttgccgg ccgctgcaag ctgcagattg atttcatgga gattctgctc
    17281 ggaatgatcg agaagaacca ggaaacgagg attgctggaa gcgagagctt gcacgagagc
    17341 ggagccgatg caacctcccg ctcctgtaac gaggatggtc ttgccggtgt tcgttgtgcc
    17401 gagcaaaggt gcgttcacgt cagagttcag tttgctgcgg aattgcaggt gcgcacactc
    17461 gagaactcaa aaggcaaagc tcaatgctgt tgattcgcga tggccaggaa gacgcgatca
    17521 tccacggcaa ccgagcgagt gaaaagggtg gcggcgtttc cagccacgat ggcgacgtgc
    17581 ctggcattgc cgctcaaagc cggaccggcg gacttttggc cggagcctgt cccatcaccc
    17641 ggctgaagca aaatccgggt cgtcttgggc gtgcagttcg ccgcaccgag acaagtatca
    17701 cggatgaaca cctggcggta gccgctgtcg ttgccgctcg cgggcgtttt taattgagcg
    17761 gaaacctggt tcgaggaatg gctcggagtg atggccagga aggcgatgaa acgacccgaa
    17821 gagctgatcg agggaagaat gctttccgtt ccaacgagag taccggcgga atcgacggaa
    17881 accaaggtgg tggaaggctt gcaggaatcg ccagccccgg cgcaggtatc gcgaagataa
    17941 atctgccgac ccgccggaga attttccagg agatttgtgg ccgcggagct gaaggcgaca
    18001 taacgaccgt cggcgctgat ggtaggcgcg tggctgtcct cattggccgc agagccgtct
    18061 gcggcgacgg agagcaaaga agtgcgcggc tggcagccgg aaggaatccc ttcgcaggta
    18121 tcccgcaaga aaatctgcgc gcgatcattc tcgagtccgg taaaggcgac gtagcgccca
    18181 tcgagggaga cggccggcgt ttcttcggca acggcggcgc tggaagccga agcctgagaa
    18241 ggaggagggc aaggctgaga atttgaacag acattgaagg gctcgccgtt gctggagccc
    18301 ccgccgccgc cggaagaggg ggcaggagga ttgcggacgg agacggtagc ggcgcctgcg
    18361 gtggccagaa aggaattggg gatggagacc gtgatggaag tggaagtgac cgtcgaaggg
    18421 gcaatggtca tttgggttga gccgaaggtc cactgaacct gcgattgctg agtgggatcg
    18481 gaggaagaaa gaaattgagt gcccgagatg ttgagcgtga aactgccgct gccgcaggcg
    18541 gagccgcacg aagtcaggtt gttgggacta aggctcgtgc tggtgaggac cgggacggga
    18601 ttgcgcggcg gattgatgat gaaagcgatg ggattggaga ggccgttttg ccccgcaccg
    18661 taggcaggat tgagagtaat gacggtagca acaccgggct tggcgaccag cgctgcggga
    18721 acagtagcgg ttacattcac gatgtttcca ccgctgtcga gggttaaggc tgtgggaagg
    18781 gactttccat tccactgcac cacggttttt gccacgaagc cccggccatt caccgtcaag
    18841 gtaaagtcgg cgcagttagc gctacaggcg ctgatctggg caggggaaag aaaggcaatc
    18901 gaggcacccg tgttgttttg aaaggtgttg ccgtagtcat tgcagccggc tacagcgaga
    18961 atcgccacga gagcgacagc cagtgctgtc caagaccgct tcattgagac tccttcatct
    19021 attctgacga ccccagccgt gccggggcca acttgcctgc gcgaacaggg ggagcccgtt
    19081 gcgctagcgc actttggggg tgagatgcca aatttgtgct gcgcgttgac cgcgacaaag
    19141 ccaagatccg gggaacgccg cccagccctt tacccacccc tgcgtgaggc cgcgcttggc
    19201 cacgaatctc aggactcggg caaccctaca tgattaccat ctggatgggg atgtggtcaa
    19261 tgcggattcg gggcgaagta gggggcggtc gctgagtatc gctcaatcct gcggctatga
    19321 ctccgtgagg acggctttct tattgcgcgc cgagtgccgc tatcgatgcg cgcaccagct
    19381 cctggacacg ggtctgacac tccctattct ggaccggcaa ggcgtttgcg gcgcgataac
    19441 aggccaacgc ttgctgcttt cggccaagtt ttgcatagag gtcgccgcgc gccagttgaa
    19501 atagcgggtt cgccgggtac ttttcaacga gtgggccgag gatgttgagc gccttttcat
    19561 actgctgatc gtaacgatgc aaattcagcg cgagataaaa acgcgcgacg ttggatgtga
    19621 gaacgccgtg cgcgatggcc tgttggagca ggcgtactcc gtcttgcttt gacccgctgg
    19681 ggatgcccat gaaaaagcgc aagatcctgg cgatggcact gagggtgtcc acgtaatagt
    19741 tgtaaagacc caggcccagg tcggcatcgg cgagatctgc atcaagagct ttggcgcgca
    19801 ggagatgttc gcgtgcgcgc actccggagc gcgctgcgtt gcgattctcg acgcgcagcc
    19861 cgtagagccg tgccgaggag gcttcagcca tgccggcata aaattgcatt tccgcggatt
    19921 cgctatgctt gatctgcgct tctgccagcg acaaggccct ggccgccagc tgaaaatagt
    19981 gccgatccgc ttcgagcttc gggcgtcgcc gggcgtcgct cattccatac ttgaaatcgg
    20041 aggaagtaca ccagatccgc caccacaagg cttccgcttc cagaagatat cccagcgggt
    20101 gattgggccg ttcctgctcc atgcgcttcg cggcctctat tgctccttcg aggtcgaagg
    20161 aatagatttt gtcgaggatg gcgggcgtct cgggtggcaa agtaagattg gaggcgcgcg
    20221 cggtgatggc aagaggcgcc aagaggagca ggcaagccag accggcgcag cgagctatgc
    20281 gcgatgcatt catcgtcccc gtccgaaaaa gagattgtgc aggttcagac tctgcgccgg
    20341 acgagtttaa gctgttcaag aagagttgca aaccgaaatg gtttttgcag aaagacagaa
    20401 ttggccggga gttctgcttc ggaaaatcct tccgcatagc ccgacatata aaccacccga
    20461 atatccgggt gaattttggc cacgcgacga gcgagttcgg gaccgcgcaa caccggcatg
    20521 acgatgtcgg tcaagagaac gtccaactca cccacatgct gttccgcgat ttccagagtg
    20581 tccggaactg tctgggcttc gaagactctg tacccggacg cgcggaggat ctccaccaca
    20641 gcatgccgca aagcagcttc gtcatccaca accagaatcg tggctccgcc gccagcatcc
    20701 tccgtgtttt cggccggaga ggaaacctga gattctggaa cagcggtctc gaccagcggg
    20761 aagtagagct ggaaccgcgc gcctttgccg ggagtactca ccacgcgaat gtggccgttg
    20821 ctctgcttga cgatcccgta cacggtggca agccccaacc cagtccccct ccctgcaggt
    20881 ttggtggtga agaatggctc aaagatctga gcctgggtat tttcatcgat gccggaaccg
    20941 gtgtcgctca cttcgaggac aacccaattc cctgagagcg agggagaatc cgcgccctct
    21001 tccggcagga ttgtggcatt gcgagtggag atggccaagc ggccaccttc gggcatggca
    21061 tcgcgcgcat tgatagcgag attggcgatg acctgctcga tctgggtggg ggctgagaga
    21121 atccaagact tcgcggcgtc atggtgaaac gtcagctcga tgtccgaacc caacaggcga
    21181 ggaagcatgc actcggattc cgtcagtgat tcgtgcaaat ccatcggatg gatctcgaga
    21241 acctgtttcc ggctgaaggt cagcagttgt ttcgtaatgg cagcggcttt ttcggtggtg
    21301 cgctggatct gatccaggta gatgcgggcg ggagattctc tggacaacga acggtccaat
    21361 aattccgtgc agccgccgag gaccatcagc atattgttga agtcgtgggc tacgccaccg
    21421 gccagcctgc cgagcacttc cattttctgc gcctgccgta cctgggattc caggcgcttt
    21481 tgttcgtctt cgagagcggt gcgtttcagg ctttgccggt agttgtcgta aaggattccc
    21541 gtcgcctgcg acatcgcctc cagatagcga acctgctgac cagtgtagcc accggggcga
    21601 ttggcgacgg cgatcaggcc gactgtttct ttgcccgtga aaatgggaac gccggcaagt
    21661 gaatgcatcg ccggatgtcc ggcagggacg ccgccggcgc gaggatcact ctgaggagaa
    21721 ttggtgataa tcgtttcgcc cttaagaatg atctcaccca agaggttgtg cttatgttcg
    21781 acttcgaagt agccgcaagt ggcgaattgg tttagtttcc cttcgaagag ttcgcgattg
    21841 agcttggtat cccacacaat tccgtcatga gccaggatgc gcaagacggg gccctcgagg
    21901 acgactccca gaaatccgta ttcgctttgc gtcttctgga tagcaaatgc cagcagatgg
    21961 cggctcgccg cggtccaatc acccacatcg aggtaagtat tgagcgcatg agtcacagtg
    22021 gaaaggagat ccgttttttc ctgcaactcg atttcgatgc gcttgcgttc gctgatatcc
    22081 tcaatcacat gcatggtgga aaccagcttg ccgttctcat cgaacatggg ggccgcggaa
    22141 atactcaccg ggattcgctc gcccgcgcga ttgaggcgca gggtttcaaa tcccttgacg
    22201 atttctccgg ccataatttc ttcgcggagt tcatttcgtt cttcttcacg ctccggcggc
    22261 gtgaaaggaa cggtactccc aacaatctcc accgctgacc aaccaaatag acgtttcgcg
    22321 gcggggttcc acaccgtgac cttgccttcc agatcagcgc tgacgaccgc cattggtgag
    22381 gcattgacga gagcgcggaa ttgctcattc ccgcggcgag cggcggcttc gcgcgcggtt
    22441 cgaacgaact ccacgatgtc gttggcggcg acgatcagaa ccacggcggt gccggcagag
    22501 gcagtagctg taacgatctt cacagcggcc aagagccagt agacgggctt ccagaccgta
    22561 accacttccg taaagtgcgt tgccccgcag ctcacgataa agaccccgaa ggcccagaaa
    22621 aaataaggat agggaaggtc ccgaccggcg cgacgcacca gccataccag agttgcggaa
    22681 atggccacgt aggaaaggcc gatgacggcg tcggcgatga cattcgacca gagcaaacgc
    22741 aggttgccga ggtagcagta ccaatgggga agaaaattcg ccccgagcag agtgcgcgaa
    22801 ccgagccaga gcagagcagt cagcgaaaag agtcccgcga gcgccgcctt cacttttcgt
    22861 ggggagacgg aaaatcgtgg ttgaggaatg ggcgaggcgc tggaggacat cgcgagagct
    22921 acctttcttt cggcgcacgc atgagaagag ccaagcgcac atcatgtggc aattcaaaac
    22981 aggggaaagt aagtgcgcgc cgcggaaaga gagatcgaaa ttccgcgagg catccgaagc
    23041 ggcttgagag gaaaagcttt cagccgggtt cgaacattgc aatcttacgc ctggtgagcc
    23101 ggttagggat gcttcgaagg ggcggtgtgc cgcaaatcaa accggtcttt gcggaagcaa
    23161 tctgggttag gcagcgcgcg gggcatttgc tattctcggc ggaccgatga cgatggggaa
    23221 caaaacggag aacacgcctc tggagcgcgg cctgggcctg aaggaggccg ttgcgctgaa
    23281 catgatcgag atcgtgggca ttggcccgtt cgtggtcagt tctctggtga ttcgggcgat
    23341 gggcgggccg caggctctga tcgcgtggct ggcgggggcg ctcctggcga cactggatgc
    23401 atttgtgtgg tcggagctgg gtgccgcgat gccgaaagcg ggcggcacgt acgtctttct
    23461 acgagaagcg tacggtccgg agcggtgggg ccgcctgatg tcatttttgt ttgtgtggca
    23521 aacgctggtg caggcgccgc tttccgtggc atcggcatcg attggtttcg caaagtatgc
    23581 gggatatctg taccccttca cgccgctgca agcgaaggca atttccggtg gattggtctt
    23641 ttttatcgtg atcctgctgt accggcggat caccgcgatc ggaaaaatct ccgttttgct
    23701 gtggattggc gtcgtgggaa cgatgctgtg gctaatctgg ggagggctca ggcacttcga
    23761 cgccaagatc gcgtttgatt tcccgccggg ggccttcgat ctgtcgtggg tgtggttcgc
    23821 ggggctgggg tccgcgatgg tgagcacgat ttacagctac tggggctact acaacatttg
    23881 ccatctcggc agcgagatca aagatcccga acggaatatt ccacgcggga tttttctttc
    23941 cgtgctgggg atcaccatcc tgtatctggc gatgcagacg agcctcctgg gcgtggtgcc
    24001 gtggcgcgag gcgcagaatt ccgatttcat cgccagcttg tttgttgaac gattgtacgg
    24061 gcaggcggcc gcgcgcttcg tgaccggaat gattttgtgg attgcggtcg catcggcatt
    24121 ctccgtcttg cttggcgctt cgcgcgtgcc ctattcggcg gcgcaggacg ggaatttctt
    24181 tccggtgttc ggcaaggttc atcccacaaa acactttccg cacgtttcgt tgttagtgtt
    24241 ggccggactc gcgtttatct tcagcatttc cttgaaattg aagcaagcaa ttgcaggaat
    24301 cctcgccatg aggctccttg tgcagttcat cggccaggcg gtgggagtga tattgctgcg
    24361 gcggcgccgg ggcacgcaag gccttccgtt caaaatgtgg ctgtatccac tgcccgcagt
    24421 gctgacgatg ttcgggtggg cttggctctt ctggcagacc gggccgacgc ggaaatgggg
    24481 actcgcggag atcgcgctgg gaaccctcgc gtatctcgtt cgcgcgcggg aagtgcgaca
    24541 atggccgttt ggaagtacgc cgccggcagt tccgagttga cggcgggaat tcgaggccgg
    24601 ctcatgcagg aggacgcgtg aatttttcgt ttcaacgact agcgatcgtg gtgttactcg
    24661 gcagcggaat gaccgcgatt cttcagacgc cggtcgccgc gcaattccct cccgctcccg
    24721 gaacgggccc gggcttggcg gaaaccattg aagcgattga ctccgcgcgc gtcaccacgc
    24781 gcatcctcta catcaccgcg catccggacg atgagagcgc ggctacactc acgtatctgg
    24841 agcgcggact gcatgcggaa gtggctttgc tttcgctgac gcgcggcgaa ggcggacaaa
    24901 acgatcttgg gccggagcag gcgccgcagc tcggattgat tcgcacgcag gagcttctgg
    24961 ccgccacgca cggttacgga gtgaaactct tcttcactcg cgcttccgac ttcggctttt
    25021 ccaaaactcc cgaggagacg gagaaagtct ggggggacca agttctcgaa gatatggtgc
    25081 gggtgattcg gacatttcag ccacacattg tgatcaacgg gtggggcggt gttcacggcg
    25141 gacacggcca ccatcaggcg gcaggactgc tcacacccaa ggctgtgcag ctggctgctg
    25201 atcccgctta caagttgcgt gaatcgacgg caggtgaaac gttatcgcct tggggcgacc
    25261 gcaggccgat ggttttactg gatctggacc ggagtgaaac accgacgggt tacatgctgc
    25321 cgctggacga agtctctcct ctctacggga agtcgtggcg cgaaatcgga ctggatgcgt
    25381 ttgcgaatca tcggacgcag ggaatcgcgg gctttctcgg gtcacccttt ctgcgacgtc
    25441 ccatcgcgct gaagcaagag gacggtggcg cgttcgatcc tgcggtgctt gcagaaccgc
    25501 ttgggccgct cgatgaagac tatgaggtgg ggaacaaaag tgtcgatccg ctaatgcgtg
    25561 cagtggacca gagcttggtg gcggcacgtg aagcggcttt gcggctggat tggaaggcag
    25621 cagcggcctc tctcgctggg gcaggcagaa aaatcagcga agtgccgacg ccgtcggacg
    25681 cggcgcaggt tccggccccg gttgagtctc tcggccgaag catcaagaag aaacgcgaaa
    25741 aaattgacat cgcgctggcg ttggttgccg ggctgcgact ggatgcgaat gcggaccgga
    25801 gcgaactcgt accgggtgag acgttcagca ttcgagtgga cgcgcatcat cggcagggca
    25861 tcaccggaga attcaagaaa acaggcctgc tgcttccgcc ggattggagc gtcgtaaaag
    25921 aagaatcgga agcgggtggc gcgattcgct ggacgatcgc tgtggcacag aatgcgcaaa
    25981 ctgcaagcga tggttccggg cggccgtcga attctcgaaa tgcctttgcc aatttgttgc
    26041 cggagccgcc accacttgtg acggcgatgc aggaggcggt gctggacggc tacgcgttta
    26101 cagtcagttc gccggtgacg aacgcgcatg cgacatccac gcgcgtagac cggattgcgc
    26161 cacggattgt gccggcgtat acgctggcgg tcgaaccgaa acacgtcgtc gaagttctcg
    26221 caaagcaaag aaaaccgttc gacgtgctgt tgcgggtgca ctcctacgcc acgcaaccgg
    26281 ggaaggtcag cgctagtgtg gatgtgccgc ggggatggaa ggcgagcgcg gcggtgccac
    26341 tgaagttcag cggcgcggga gatcagtacg cccgcctcac ggtgactcca ccgatcaagc
    26401 ttgtcgcagg gaactacaag attgccgcgc atgcgattcg aagcgcggac cgcgcggcgg
    26461 gagggaaggc ggagaggtac gggacgtcgc tcgaaccttt gccgacgctg cctacactgc
    26521 tgtggagcga gccggcccaa tgcgcggttc attcgtttgc tgtaagcgtg cctccgaatt
    26581 tgcgcgtggg atatatcacc gcagagagcg agccgatccc ggaagcgctg cagcgactgg
    26641 gcattcacgt ggagacgcta gatgcgcggg ccttggcttt cagcgatttg tcaaaattcg
    26701 gggccatcgt cgttggggtg cgcgcctacg agttgcgccc ggagctgcca ggcgcgaacc
    26761 agcggctgct cgattacgtt tccaagggtg gcactcttgt ggtgcaatac cagcgcgaat
    26821 tcgtctggga caaaacgcag tttgcaccgt ttccggcgct catctcgccg ccgattccgc
    26881 tcgtgaaaga tggcgcgcct caaacgccgc ggccactgcc gcgcatcacc gacgaaaatt
    26941 ctcccgtcaa atttctgaaa ccgcatgatc cgcttctgaa cacgccgaac aagatcacgc
    27001 aggatgactt ccggggctgg gtgcaggagc gtggactcta tttctggacg cagttcgacc
    27061 cgaaatatac gccgctcctc gcgatgaacg atccgggcga atcggacctg aacggcgcgc
    27121 tggtttacac gcgctacggc aaaggcactt acatctacac gggtcttgct ttcttccgtc
    27181 aattgcccga aggcgtcccc ggggcttacc ggcttttcgt caatctactc agcgcatcac
    27241 ggagtcgctg agcccggtcc accgcgaaaa acctcgggcc tgaaagccta agctagaact
    27301 gcctcgagta agtaaaccaa ctgggcacat cgctgccgca cagaaatcat cgtgctattc
    27361 tcttgagccg aggggtgaaa cgcgaatgag caatcatcat ggaactcgct ttgccgggcg
    27421 aacggttcac caggtcattc tattgttgct ggcaaccgcg ctctctttga cttgcgtttc
    27481 tcccgctgcg gcacagaaaa aaaagaagtc agaccctcct ccgtccacgg accgctccaa
    27541 gatgctggtt tctttgaccg acgaacagca gatcgattac acgctctcgg aaatgctggg
    27601 agcctggcag ctcggggata tcgaaaagct gcgcagcgac tacggcgacg atgttgcgat
    27661 tgtgaacggc tcgtgggcgc cgccgattct cggctggacc aactatcttg ctgtttacca
    27721 gcagcagcgc gcgcgcatgc agcaggtgcg catggatcgc acaaatacct acatcaaggt
    27781 gagcggtacg gtgggttggg cgtgctacca gtgggacttc gaagcagttg tggatggcca
    27841 gcagatggca tcccagggcc agactacggt ggtgctggag aagcgcaaca accactgggt
    27901 cattgtgctc aatcacactt cgatcccgcc aagaatcccg caagccgctc ctgcgaacgc
    27961 accttctggg cagcagcaac ctgcgaagcc gacgccgcgc tgaagcctgc cggcaaactt
    28021 acggaaacct gaagacctag aagaggccct tcagcccttc cttgagccac attccggcaa
    28081 tcaccagcag cagaacgcct gccaagatgc cggtccccat gatgggccgc gagagggcgt
    28141 tgatcttcgc cacaagctcc cgctgctctc tggccatatg ttcttccgct gcatcgagaa
    28201 agatttgatc gtcttcgtgg cttgcgagga ggctccgccg aagcaacaga accagaaatg
    28261 cggcggtaac gattccccag gcgatgagca tgtagagcag tggccccgtt agcgtttgtg
    28321 attccatggt gacctcccgt tggaaccgaa ccgcggtgcc ggccgggccg gcaaggggta
    28381 aatagggttt tctagagcgc agtatagcac tttggattaa aaattctagt gctgagaatt
    28441 ggcaagactg gtgctccacc atcaatttga ctgcttgggg acctgggctt tggagctgcg
    28501 gtatacggcg gcaaaaccaa caaactaaag cggatttttg atccgctacg ccctcggaat
    28561 cgcgggtgag cggccaaccg acggcggatg gggtctctgc gcccacggag ctgcgggcgc
    28621 tcctgagcgc tagcattctg gtcgcgttga caaaggagga gccgccgggc aaaatgaaaa
    28681 tgctttgttg tgtgtgtctc agagcggatg gggtcataca ttccaagcgt caccgcgaag
    28741 cggtgacccc gcacttgtgc ccgaatggcg gaattggcag acgcgctagc ttcaggagct
    28801 agttctcgca agggagtgga ggttcgagtc ctctttcggg caccaattat tcgaaaatag
    28861 gaatccttct ttcgcacgat tgacgatttg ctcgggaatc cgttacggta ttcccgcaga
    28921 ctgatccgga tcacagccga ggtgtcgcta tggttttcct tctcctggcg ctttcatttc
    28981 ccgagtggcg gccgcacttc tctttcctcg atactccgca cgccaacgaa atgttggcgc
    29041 tacgctggct tcaccttgtc ttcgggatca tctggattgg cttgctctat tttttcaatc
    29101 tggtgctgac gccggcgatg aagaagtgtg atcccaagtt gcgaatcaag atctatcccg
    29161 agctgatgcc gggagcgatg gagtggttcc gctggtcggc actggtgacc gtggtagtgg
    29221 gaatccgcta cttcacgatc catctggcct cggatgcgaa acttgcgggc gatcgttcgc
    29281 tgactggcac gtggttcggt tggtggttgc tggtgtggct cgccgcgtat gtgttcatct
    29341 acgctttgca actccccgcg aaggggatct tcaacagccc gtggtttcgt gtcatcggag
    29401 tttctcttgt tgtgattacc gcgtcctggc tggtgcttgc tttgaatggc gggcccaatg
    29461 tttcgaacgc gcaccttgcc atcagtgttg gcggtgggct tggcttcatt atgctgctga
    29521 atacttgggg agtcatctgg cgtgttcaga agcgtctcat cggttgggcg cgtactagtg
    29581 ccgagcacgg tgctccgatg ccgccggaag cggagcgctt gatgcgctgg aactatctta
    29641 cggcgcgcac ctccttctgg ctgtcctttc ccatgctctt cttcatgggc gccgccagcc
    29701 actatccgtt tctgagcagc atcgcgcgat aaatcgggcc gaaggcttcc atgtctgatg
    29761 aaaagaacta taacgtcctc acagctgaaa ccggcgtgcc gattaaggcc tggaccaaag
    29821 gagtccagct cgaagatcag gcgcgcaagc agcttctgaa cgtcgcccag ttgcccttca
    29881 tctttaagtg ggttgcggcc atgccggacg tgcactgggg cgtcggggcc acggtaggca
    29941 gcgtcatccc cacgaagggt gcgatcattc ctgccgcggt gggcgtggat atcggttgcg
    30001 ggatgatggc tgtgcaaaca gacctgaacg cgcgggatct tcctgacaat ttgaagggca
    30061 ttcgcagcgc catcgaacag gcggtgccgc acggccgcac gaatcacggc ggccacggcg
    30121 acgtgggtgc gtggcgggag attccttcgc gcaacaacga agtctggcag acgctaaagc
    30181 cgcgctacga cgccattctg gaaaagcacc cgaagctcga tcggggcaac cactcaaatc
    30241 acctcggcac gctgggcacc ggcaaccact tcatcgaagt ttgtctcgat gaagcggaaa
    30301 acgtctggtt catgctgcac agcggttcgc gcggtgttgg caatcgcatg ggcagctact
    30361 tcattgaagt ggcgcgcaag gacatgcaga agtttttcat caacttgccg gacaaggatc
    30421 tggcctattt ctctgaaggc accgagcact tcgacgacta cgtcgaagct gtcgaatggg
    30481 cgcaggactt tgcccgcttc aaccgcgacc tgatgatgga gcagatcgta ggcgtggtgc
    30541 gcaattccgg tgaagtgcgc ccgttcatcg cggggctgaa ggccatcaat tgccaccaca
    30601 actatgtggc acgcgaacgc cactacggcc agaacatcct ggtcacgcgc aagggcgcgg
    30661 tccgcgcccg cgaaggcgac atgggaatca ttcccggcag catgggagct cgttcctaca
    30721 tcgtccgtgg caagggcaac ctggaaagct tcatgagctg cagccacggc gcgggccgcg
    30781 cgatgtcacg cacagaggcc aagcggcgct ttaccgtgga agaccacaag cggatgacgg
    30841 ctggcgtgga gtgccgtaag gacgctgacg tcattgacga aacgccggcg gcttacaagc
    30901 cgatcgaggc tgtcatggcc gcgcagagcg atctcgtgga aatcgtgcac accttgcgcc
    30961 aggtcgtttg tgtaaaaggt tgaccgagga acaaaaatga aaagggaggc gggccaggtc
    31021 gtcccgcctc ccttctttcc cctctcgggg aatccggcta gtctgacgga gggcgactag
    31081 ccggtcgtgt gttggcctgt ctatataatc ccggaatcgt gaaaaaagtt cctgagaaac
    31141 ccaaaaaagt gactaaaatc acggtggaaa tcagcgcctg ccgtctggcg cgaaagccca
    31201 tgccactccc ctcacacctc agccccgata acatattgcg ttttctgcaa gttagaagcg
    31261 agcccgcgtc cacgagcgag atcgcgcagg gactccatct aaaaaagagc gacaatcgcc
    31321 cactcttcaa gatgctctcg aagctcaaaa agcgccgggc cattgaggaa ttgcttggcg
    31381 ggcgataccg ccttccaggc cgcaagtctg aacgggaagc ggccacccaa aaactgtcgc
    31441 aggacgcggt gcggcatccg caacactcgg cgctcgcgga tcgtgatgaa gtaaaaggac
    31501 gcttggttct tcaccacgac ggctatggct tcgtcgtgcc ggaatcgccg atgccgcacc
    31561 tcgacggcga tattttcatc ccacgcgacg ccattcaaga cgccatgcac ggcgaccacg
    31621 ttctggcaaa gatccaacga ttgggcggcg tcacaggtgc gcaacgagct gaaggccgca
    31681 tcattcgaat cctgggccgc gcgcatccaa cggtggtcgg tttgtttcgt tacggcaccc
    31741 aacaaaatgt ggtcctgcct tacgatgttc ggatccaaca cgaggtggag attccccgcg
    31801 gcaacgaact gactcccggg ctatggaaaa agctgggctt cagcggcgcg gatgaaacca
    31861 gcatccgatc gcggcgcatc ccgcggatgg atgaactgga cggcgccgtc gtgaacgtgg
    31921 agttgctgcg ttatccgcag ggaggcgcag cggcgaccgg ccgcgtcatc gagattcttg
    31981 gccgtcccgg cgatctcggc gtcgacatcg agatcatcat ccgcaagcat catctcccgc
    32041 attccttctc cgccgaagtt ctggatgaag cggagcatgg cgcgaagccc gtgagtgaaa
    32101 cccagcgtgc cgaccgcgaa gatttccgcc acttgccgat tgtcaccatc gacggagaga
    32161 ccgcgcgcga tttcgacgac gccatccacg tcgagcatcg cgacgatggc ggctggcacc
    32221 tgcaggtcca catcgccgat gtagcgcact acgttcgaac ggggagcgcg ctcgatcggg
    32281 aggctcgcct ccgcggcaca tccgtttact ttcccgatcg cgccgtcccg atgcttccgg
    32341 aagccttgtc gaacggcatg tgttcgctca agccccagga ggatcgcctg gtgatgagcg
    32401 cgctcatgga attcaacgcc gcaggccaca tgaactcctc tcgcatgact tccggcgtca
    32461 ttcgctccgc cgagcgcatg acctacacga acgtcaacag agtccttgaa ggggatgcgg
    32521 aaatgacgga gcgctacgcc gtgctggcgc cccatttccg cgaaatgaaa gaactggcac
    32581 tgctcctcaa tgcccgccgc aatgagcata gttccatcga tttcgatctc cccgaaccgg
    32641 tcatcgaatt cgacgaagag caacgcatga ccaatatcac gcgcagcgag cgaaacatcg
    32701 cgcaccggct cattgaagag ttcatgctgg ctgccaatcg cgctgtagac acctatctcc
    32761 tgcagcgcag catcgaatcg ctgcatcgcg ttcacgagaa gcccgacgca cgcaaagtcc
    32821 tggagttcga agagctggcg cgtgccttcg gttactctct tggcgtcgaa gacctgcacc
    32881 agcgcgaagt cgccgttcgc catggccgtg tcccggcgcc cgcgaaagcc ggccgccccg
    32941 actcctacgg ccacggccgt gagcgcggca tgaaggtggc gctcccgggc ggcgagctgc
    33001 gcattacgcc gcagcattat cagcgcttga ttcgaaaggt ggttggcaaa ccggaagaac
    33061 gcatcatttc ctacctcatg ctgcgttccc tcaagcaggc acgctacgcc gccgagcctc
    33121 tcggccattt cgccctaggc ttcgacgaat acacgcattt cacttcgccc atccggcgat
    33181 atcccgacct catcgttcat cgcattctga aatgggctct ggatcatccg aaagatgccc
    33241 cggcctcttc aaagacgcat gtcgccgctt cgctagggtc tgagtccacg ctctattcgc
    33301 acgggaacct cgaagaaatt gcttcagaaa cgtccgaagc ggagcgccgt gccaacggcg
    33361 ccgagcgcga acttatggat tggaaaaccg cccaattcat ggaggagcac atcggcgagg
    33421 aatacgacgg gctgatcatt tccgtacaga aatacggctg ttttgtcgaa ctattcgagg
    33481 tcttcgtgga aggccttctt cccgtcgggg cgctggagga attcgccggc acgcgatgcg
    33541 ttcttcgcga gcgagaccac gccatcgttg ccttgccggg tggcggcagc cgcggagccg
    33601 ccggcgcccg ccgtggccgc ggcgcaaagc cccgtcagct cgaatggcac cacggcgata
    33661 aagtccgcgt gcgcgccgag cggatcgatc ccatgcgcaa acgcgtcgag tttgcgctgg
    33721 tttaagagaa atgagggagg aagcccggga attcagcgaa ccgctgcgct tgagatatgc
    33781 agtgcgttca tcacccgccg caccggccgc gtcactgcgg cggcgaattt cgccagcgga
    33841 taccaaaacc ggctatgccg cgacagttgc gagatgaata gtcccagagc cagttggcgc
    33901 ggcgcatcaa gatcaatccg gcggccattc cgataaattc gcatcacgcg gcccaatagc
    33961 tcctgttcct ggacgacgcc atcggaggtc ggatgcgcat cgcctttgga aaaaagctgg
    34021 gcggcattca gcgagccacg cttctcgaca atgcggtgca cgaagagatg atgttcgcgc
    34081 cggaacaaga cgacgtcccc gcagcggaca ttctcaattg agatttgccg gatcagggcg
    34141 atgtccttcg gccgcaccca gggctgcatg ctggtgccgt gaacgcgcag ggaaatccgg
    34201 ccacgccgct tcagtgcgtc cgcgatgcgt agcgcatcgt cggcgcggcg ttccccgctg
    34261 atccgattcg tttgctttcc ctggtctttc cgattctcca tcaaacctcg ctaccgaaat
    34321 tccattggcc gggtctctat gttccagcca tccagcgaga tgcgtgaacc tttgcacatg
    34381 ttgaagcgca ggcacgcata atcttggcct attccgaact ggaacaagta cggaggagtc
    34441 gttcggaatc gaaactggtc caacttctgc tatcagagtg gtctctcgga ggggaacggt
    34501 caatggacgg gaagtacttg cttgcccaaa gctacagcac tcaatcgaaa cagtttatcg
    34561 gatgttacga gcgcccctga agggtgcctg aggtttctct acgaccggcg cggagcaccg
    34621 ttacagacgc caccctgacc cggcaatttt ccgcatgcca gcgccagcgt ttcgtagatc
    34681 ggttccgaca caacgctcgg cttcgtatac ggcttctttt cgccgggcgc tgggggagag
    34741 ttcttgtttt cttcagccat tcgatctttc cttagtctga accagaatcc gtagcaagag
    34801 aagcatattc ggcctcagga gttgtctgaa ctagagttgt attttgttcg acatgaaatg
    34861 cgctgatggg agcagcgaca cccgccagtc tcgctttttc ttccgcgagc gcgcaagcgc
    34921 gctcgtaagc cccaagcaaa tccccgcctt ccatcagcgc caggcccggg cagcgctcgc
    34981 agtagctccg aatctcgcat cgcgaacaga tgggaagctg gctctcgcgt atagcgcgca
    35041 accgttcgag ttgcggcgcg tgataccaga tgtcctcgaa tttctctcgc cgcagattcc
    35101 ccgctgcctg gggcaattgc acgcacggaa agacatctcc gtagggcgaa atgtaacaac
    35161 tgttgtggcc cgcgctgcaa ggcaagtctt cgtaagctag actctcgatt ccgctggaga
    35221 cggcgctccc aaacgacggc ggcagggcat tcatggcgct ggctgcttgc gcgcttggct
    35281 gcggtttgca ggcatgcagc gcaggatcac gcagtaccgg cagcagcgac gaaaccgatg
    35341 cgcggtgcgc cagcggtccg ctggagccgt ctatcatcgg cgtgatcgtc agatccagaa
    35401 tgtagggaac gcccagcttc tcagcgagcg ccatcacgcc gcggtaggcc atcaagtttt
    35461 cctgcatcaa cgggcaagcc agtttcacct gcattccgtg ctcgagcaga atgggaatcg
    35521 ccgccagcgt ccgctgcagc gatccgggaa ccttggtgat ggcgtcgtgc accgcgggaa
    35581 tatcgctgta aacgctgatc tgcacgcgcc gtacgccaaa ctcgcgcagc cgcgccgcgc
    35641 gttccggcgt gaccagcaag gcgttgctct tcaagctgat gtcgaaatgc aggcgtctcg
    35701 cggcggccag aatttcgtag agatccgggc gcaggaaaat ttcgccgccg ctgaacgtca
    35761 aaaacagcgc ccccgagcgc gccagatcat ccagcacttt caggcactcg gccgtggtca
    35821 tttctccatg atcgtcatga tccagataac agtgcacgca gcgctcgttg cagcggtacg
    35881 tcaagtcgaa atgcacgctc agcggccggt gcttctccac ggtgcgtgag atcagacgat
    35941 ccatcacggg cgtggcgctt gctgctagag tcgcggtcaa ggcgatactc cccccgtcgc
    36001 cgggaccgtc accactagtt tccgtgaaga catctgttcc aagagcgctt gggtgtcgga
    36061 taacgcgatg tccggcgtga cttcgtaatt ttcagccagc agttctgcca gctccgccgc
    36121 cgacttcttt ccatcaatgt tcttccagag gaaactcccc gtatcgttga gctcgtgcat
    36181 cacgctgtca ttcggcgaaa tgatgattgt ttcgtcgtca atttctctcc atgccagggc
    36241 cggattcttc tgccaagaaa cagaggctgg agtgctcatg cgggaagaac ctcccaaaac
    36301 gtcgagtttt tgcggaatgc caggttatag ccggctattt cggtcacggc ctgatttaga
    36361 atttccagaa gctgccggtt ggcttccact tcggcggaga agaaaagcac attcggcagg
    36421 agggcgcgaa gaatcgccac ggggcggagt gtttccaccc gattttccgc ggcctgcaag
    36481 agacgaaaaa tccccgccac ggggacactg gtatttgatc ctgcggcgcg gaactcgccc
    36541 caaaaaggag ttccgtacgc gcgccacgct ccatcttccc ggcgaagcag ggaaatctcg
    36601 tcggtgagga cgcttccttc cggagacaac gatgccaccg tgctcttccc ggcgcccgac
    36661 ctgcccgcaa aaatgtatgc ttttccgttc cgaacgacgg tggccgcgtg cagtagaaat
//
LOCUS       DASYVO010000005         5395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_141121, whole genome shotgun sequence.
ACCESSION   DASYVO010000005 DASYVO010000000
VERSION     DASYVO010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141121"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="VGR03_00250"
     CDS             <1..1455
                     /locus_tag="VGR03_00250"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEV1992741.1"
                     /translation="DIFRVLPELVWCGFGVLVMLMQPFVRSRHFFTFLALIGTLAGTA
                     ASVTAAMHAGPGFGGLVQSDSFSFFFRLLVGTVAFLVVLAAGPYLERERLPFAEFYAL
                     LLFATAGMGVLASAQELLTAFIGLEMSSISSYVLAGYRRDSLKSTESAMKYFLLGSFA
                     TAFFLYGIALVYGSSGTTMLTRMGSEELLHSTLFELGLAMILIGLGFKVAAAPFQVWT
                     PDVYEGAPTPVTALFSAGPKAAAFALLLRIFATVPAATHFWFWAFWALAALTMFAGNL
                     GALVQTNVKRLLAYSSIAHAGYILVAFAAVTSMAQGEGGSANAAPAYAAVLFYLLSYA
                     LVKLGAFTIVSQLGGAGEKNLSLDDYAGLSQRQPWVAAALSVYLLSLLGLPVTAGFFG
                     KFYIFKAAVNSHLLWLAVLMAINSVIGAYYYLRVIVVMYMREPSAEAASAAPVGFPLT
                     VNFVLAFTVVGTLYFGLFPNQVLNFILQPSLLGR"
     gene            complement(1600..1914)
                     /locus_tag="VGR03_00255"
     CDS             complement(1600..1914)
                     /locus_tag="VGR03_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEV1992742.1"
                     /translation="MKKWSMMLMAILAVMMFAPAAFAQDNAGGAAGGHAALWISAAFG
                     MGIAAFGCGLAQGKIGAAALEGIARNPGAAKNIFTPMLLSLAFVETLVLFTFLMIILK
                     VK"
     gene            complement(2022..2840)
                     /locus_tag="VGR03_00260"
     CDS             complement(2022..2840)
                     /locus_tag="VGR03_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit a"
                     /protein_id="HEV1992743.1"
                     /translation="MEENISPITHLVNHYLGPMALAILQALHIQPENRDLPFPEHVIM
                     GVLVLVLTAILALILKSRLSVEKPGAMQQVAELLITNPMRLGIQDILDESAGHHARSF
                     IYFVGTVSIFVLFSNLFSLFPWFSAPTGNVSVPLACAILTFIYFNWQGIRHQGAVSYL
                     KHFAAGTEWWIAWLILPVEVISTLARLLSLTVRLYANIFASDMIYVLFLGLLSQASTL
                     AWQKSPVAGILVGIFPALIPLLFIALHLLVAFVQAILFTVLPASYLGMATAEEH"
     gene            complement(2871..3227)
                     /locus_tag="VGR03_00265"
     CDS             complement(2871..3227)
                     /locus_tag="VGR03_00265"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEV1992744.1"
                     /translation="MIPTFGLLAGLLAGLLHKWDWAEGLVLGSGLAWLNFRWLRRGLE
                     AFTSAAAPQVGPEKPRAHVASYLAAAFRYVLIGLTVYVIFSYLHVPLVSIVLGLCAFA
                     AATIAASVWEIFQSVE"
     gene            complement(3280..3510)
                     /locus_tag="VGR03_00270"
     CDS             complement(3280..3510)
                     /locus_tag="VGR03_00270"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEV1992745.1"
                     /translation="MQSTSTQLAMAMEVPFTFVGAVAVGGLIGYFLDKWLHTGPWLMI
                     VFGCFGFAAGILEIARRFGPKDSGSNNDKPAI"
     gene            complement(3551..5149)
                     /locus_tag="VGR03_00275"
     CDS             complement(3551..5149)
                     /locus_tag="VGR03_00275"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEV1992746.1"
                     /translation="MNIPFINAILTYLQGDGAAILPEIELVLFGLGILLIDFWIDQKE
                     KYWNAGLALAGTIFSAFTLWRLRGTIAARGDLVGFHETVLVDPFFLFFAALFLAATAL
                     VILLSVKYLEIEREQEGEYYALLLFACVGMMFMASGVDLIVMFLGLETMALSFYVLTG
                     FLRSEKRSNEAALKYVLLGAFSSGILAYGFSILYGLSGATNIGAITAVLAQRDKLVHV
                     VALSHLPGAQGEQMHELLARQLPIALNFDPFVLQLLPILAFVLVAVGLFFKVAAAPFH
                     QWAPDVYEGAPTPIAAYVSVASKTASFALLLRLFLSVFSPSQRVSHDQWMYLIAGVAV
                     ASLTWGNLAALTQTNVKRLLAYSSISHVGYILLGLVAGNGTALTGIMYYLFAYVFMTA
                     GAFAVIIVLRQKGIIGEELDDLNGLYQRSPAAALLLLIFMLSLAGIPPTAGFMGKYFI
                     FLSLIETHHPVLAVFAVLYIVPALYYYFRIVVHAWLKQPGEAPRPVMTSAQAVALGVA
                     VFVTLAAGLYPEPFTRLAQYAFGQ"
     gene            complement(5159..>5395)
                     /locus_tag="VGR03_00280"
     CDS             complement(5159..>5395)
                     /locus_tag="VGR03_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992747.1"
                     /translation="PVTQFANEDLPDLNLREYATLVPLVILAFWIGIYPKPFFAIIEK
                     PVQRIVEQVNPNFYQAERAKLPPAELHAAAAETK"
BASE COUNT         1182 a         1481 c         1649 g         1083 t
ORIGIN      
        1 gacatttttc gcgtgcttcc ggaactggtg tggtgcggct tcggagtgct ggtgatgctg
       61 atgcagcctt tcgtgcgcag ccgccacttt tttacgtttc ttgcgttgat cggcacgctt
      121 gctggaacgg ctgcatccgt caccgcggca atgcacgcgg ggccgggctt cggcgggctg
      181 gtgcagtccg attccttcag tttcttcttc cggctgctcg tgggcacggt ggcgtttctg
      241 gtggtgctgg cggcggggcc gtacctggag cgagagcggc ttcccttcgc ggaattctac
      301 gcgctgctgc tctttgccac ggcgggcatg ggcgtgcttg cttcggcgca ggagttgctc
      361 accgcgttca tcggcctgga gatgagttcg atctccagct acgtcctggc gggatatcgg
      421 cgtgattcgc tgaagtccac ggaatcggcg atgaaatatt ttctgctggg ctcgtttgcg
      481 acggcgttct ttctctacgg cattgcgctg gtgtacggct cgagcggaac gacgatgctg
      541 acccggatgg gctccgagga gcttctccac agcacgttgt tcgagcttgg gctggccatg
      601 atcctgatcg ggctcggctt caaggtggct gcggcgccgt tccaggtgtg gacgccggac
      661 gtctacgaag gcgcgccaac gccggtcacg gccctctttt ccgcgggacc gaaggccgcg
      721 gcgtttgctt tgttgctccg catatttgcg acggttcccg ccgccacaca cttctggttc
      781 tgggccttct gggcactcgc ggcgcttacg atgtttgccg ggaatctggg tgcgctggtg
      841 cagacaaacg ttaagcggct cttggcgtac agctcgattg cgcatgcggg ttacatcctg
      901 gtggcgttcg ctgcggtcac ttccatggcg cagggcgagg gcggctcggc caacgcggcg
      961 cctgcttacg ctgcggtgct cttttatctg ctcagctacg cgctggtgaa gctgggggcg
     1021 tttacgatcg tttcgcagct gggcggcgcg ggcgagaaga atctctcgct ggacgactat
     1081 gccggactct cgcaacggca gccgtgggtg gcggcggcgc tgagtgttta cctgctttca
     1141 cttctcgggt tgccggttac ggcaggcttt ttcgggaagt tctacatctt caaggccgcg
     1201 gtgaactcac acttgctgtg gctcgcggtg ttgatggcga tcaatagcgt gatcggggct
     1261 tactactacc tgcgggtcat cgtggtgatg tacatgcgcg agcccagcgc ggaagccgcg
     1321 tcggccgctc cggttggatt tccgcttacg gtgaattttg ttttggcctt cacggtggtg
     1381 ggcacgttgt acttcggact ttttcccaat caggtgctga acttcattct ccagccaagc
     1441 cttctcgggc gctgataacg caattccaaa aaagaagggc ggactgaact ctgctcctaa
     1501 atcggatgcc agccatcagc ggatggcagc catgtgaaaa gaaaatggcc cggaggcttg
     1561 ggccgccggg ccggcttcgt gacagcactt ggttaagtct tacttgacct tgagaatgat
     1621 cattaaaaag gtgaacagaa cgagcgtttc aacgaaagcc aagctcaata gcatgggagt
     1681 gaaaatattc tttgcggctc caggattgcg cgcgatgcct tccagtgcag cggcaccaat
     1741 tttgccctgt gcgagcccgc aaccgaacgc ggcaataccc atgccaaacg ctgccgagat
     1801 ccacagcgcg gcgtggcccc ctgcggcgcc cccagcatta tcctgcgcga acgcggccgg
     1861 ggcaaacatc atgacggcga gtatcgccat caacatcatg ctccacttct tcattgactg
     1921 cctccttatg aattgccgcc aggaggtggt tcccggacac cgtgcagccg ggctcacact
     1981 ggatcggcaa gttgccagcg aacctcgcca gcaaatatcg actagtgctc ttcagccgta
     2041 gccatcccga ggtaggaagc cgggaggacg gtgaagagga tcgcctgtac aaaagccacg
     2101 agaaggtgaa gggcgatgaa taacagcgga atcagcgccg gaaagattcc aacaaggatt
     2161 ccagccaccg gggatttctg ccaggcgagc gtggatgcct gcgagagcag tcccaggaac
     2221 agcacataga tcatgtcgct ggcaaatatg ttggcataga gtcgcacagt aagtgaaagc
     2281 aaccgcgcca acgtgctgat gacttccacg ggcaaaatca gccaggcaat ccaccattcc
     2341 gtcccagcgg caaaatgttt gagatagctg actgctccct ggtggcggat accttgccag
     2401 ttgaaataga taaacgtgag gatggcacat gccagaggca cgctaacatt gccggtcggg
     2461 gcagaaaacc atgggaacaa actgaaaaga ttggaaaaca gtacgaagat actcacagtg
     2521 ccaacgaagt agataaagga acgcgcgtga tggccggcgc tctcatcaag aatatcctgt
     2581 atccccaagc gcatgggatt ggtaatcagg agttccgcaa cctgctgcat tgctccgggc
     2641 ttctcgacgg aaagacgcga cttgaggatc agagccagga tggccgtcaa aacaagcacc
     2701 aaaactccca tgatgacgtg ctcaggaaag ggtagatccc ggttttcagg ttggatgtgc
     2761 aacgcctgaa gaatcgccag cgccatgggg ccgagataat ggttcaccag gtgcgtgatc
     2821 ggactgatat tttcttccat ctcttgcacg cccgttcagg gctcaactcc tcattcgacc
     2881 gattgaaaga tttcccacac acttgctgcg attgtcgcgg ctgcaaaggc gcacagtccc
     2941 agcacgatgc ttacgagggg aacgtgtaga tagctaaaaa taacatacac cgtcagacct
     3001 atcaagacgt agcgaaaggc cgcagcaagg tagcttgcca catgcgcacg aggtttttct
     3061 gggccaacct gcggcgcagc agcggacgta aatgcttcca atcctcgcct tagccagcgg
     3121 aaattgagcc aggcgagacc agagccgagg acgaggccct cagcccagtc ccatttgtgg
     3181 agaagaccag ctagaaggcc agccagtaag ccaaaagtcg gaatcaagta ggcgattcgg
     3241 cgtacggttc tctcgcctac gaggggaacg gttgatggct cagatggcgg gcttgtcatt
     3301 gttactgccc gaatccttgg ggccaaacct tcgagcgatt tcgagaatcc cggcggcgaa
     3361 gccgaagcag ccaaagacaa tcatcaacca agggccggtg tgcagccact tgtcgagaaa
     3421 atagccaatt agccctccta ccgcgaccgc ccctacgaag gtgaacggca cctccattgc
     3481 catggcgagt tgtgtcgaag tgctctgcac tttttggagt ggaggcttcg gtgattcgga
     3541 gttttccggt ttattgtccg aaggcatatt gggcgagccg cgtgaacggc tcaggataca
     3601 acccggcggc gagcgtgaca aacacggcga cgccgagggc cacggcctgc gcgctggtca
     3661 tgacggggcg tggcgcttcg ccgggctgct tgagccaggc gtgcacgacg atgcggaagt
     3721 agtagtacag cgcgggaacg atgtacagca cggcgaagac agcgagcacc ggatggtgcg
     3781 tttcgatcag cgaaaggaaa atgaaatact tgcccatgaa gccggccgtc ggcgggatgc
     3841 cggcgaggga aagcatgaag atcaggagca acaatgccgc ggcggggctg cgttggtaca
     3901 aaccgttcag gtcgtcgagt tcttcgccga tgattccctt ctggcgaaga acgatgatga
     3961 ccgcgaacgc tccggcggtc atgaacacgt atgcgaacag gtagtacatg attcccgtta
     4021 aagcggtccc gtttccggca acgaggccga gcaggatgta gcccacgtgc gagatcgagg
     4081 aatacgccag gaggcgcttg acgttggttt gcgtaagcgc ggcgagattg ccccaggtca
     4141 gagacgccac ggccacgccc gcgatgaggt acatccattg gtcatgggaa actctttgcg
     4201 aaggtgaaaa gacggaaagg aatagccgca gaagcaacgc aaaactcgcc gtcttggatg
     4261 ccacgctgac ataagcagcg atcggtgtag gcgcgccttc gtaaacatcc ggcgcccact
     4321 gatgaaacgg cgcggccgcg actttgaaaa acaatccgac cgcgaccaga acgaaggcga
     4381 ggatcggaag aagctgcaga acgaagggat cgaagttcag cgcgatgggg agttgtctcg
     4441 ccagcagttc gtgcatctgt tcgccctgcg cgccggggag atggctgagc gccacgacgt
     4501 gcactaattt gtcgcgctgc gccagtaccg cggtgatggc gccgatattc gttgcgccgc
     4561 tgaggccata cagaatcgaa aaaccgtagg ccaggattcc ggagctgaac gcgccgagca
     4621 gcacatactt tagagcggct tcattcgaac gtttttcgct gcgcaagaaa ccggtcagaa
     4681 cgtaaaagct gagggccatg gtctccaggc cgagaaacat cacgatcagg tccacgccgg
     4741 aagccatgaa catcatccca acgcaggcaa acagcaagag cgcgtagtat tcgccctctt
     4801 gctccctttc gatctccagg tatttcacgg agagcagaat tacaagcgcg gtggcggcca
     4861 ggaagagggc cgcgaagaaa aggaagaacg gatcgaccaa gaccgtctca tggaacccga
     4921 cgagatcgcc ccttgccgcg atcgttccac ggagcctcca cagcgtgaaa gcgctgaaaa
     4981 tggtgccggc cagtgccagc ccggcattcc agtatttctc cttctgatcg atccagaaat
     5041 cgatcagcag gatacccagt ccgaagagga cgagttcgat ctccggaagg atagcggcgc
     5101 cgtcaccctg gagataggta agaatcgcgt ttataaacgg gatgttcatg cgggcgggct
     5161 atttcgtctc cgccgcagcg gcgtggagtt ccgcgggagg caattttgcg cgttccgctt
     5221 gatagaaatt tggattcacc tgctcgacaa ttctctgcac gggcttttcg atgatggcga
     5281 agaacggctt cgggtagatg ccgatccaga acgccagaat cacgagcggc acgagcgtgg
     5341 cgtactcgcg gagattcaaa tccggcaaat cttcattggc aaactgcgtg accgg
//
LOCUS       DASYVO010000006        11130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_28284, whole genome shotgun sequence.
ACCESSION   DASYVO010000006 DASYVO010000000
VERSION     DASYVO010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 11130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11130
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28284"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..410
                     /locus_tag="VGR03_00285"
     CDS             51..410
                     /locus_tag="VGR03_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992748.1"
                     /translation="MVTPERLLRFTIELIFVLLGFLVIWLGLARHIYVDRHSASWLIF
                     GAALILWGLRALYKPGQWWLRWQNWTRGLSLALLGVLMLVISRVPFLWVGPMLAAGGG
                     LLVLRGMIAAFLIFRPR"
     gene            complement(740..3010)
                     /locus_tag="VGR03_00290"
     CDS             complement(740..3010)
                     /locus_tag="VGR03_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV1992749.1"
                     /translation="MSSTPNSSAAAGGRGSGIPRVIVATTALLTFISFWRAAAIVLND
                     LASSAYYAGGEAEGYIGKTAPWFILAIMLFSYAVRAVYVESCSMFVRGGVYRAVKMSL
                     GGTLAKFSVSALMFDYILTGPISGVAAGQYLAGLINDLLQYFHVSGALSSGTISFIAA
                     SFAIVVTLYFWWENTKGVAESSEKALHIMKLVTVMVVMLIAWCVYTVAVRHSALPPWP
                     QPRNLRLSTSALGWLAHSSLPHMFGIIAIFIALGHSVLAMSGEESLAQVYREIESPKL
                     PNLKKAGAVIFIYSLVFTSLVSFFAVMIIPDDVRQHFLENLIGGLAMNLEGPFFARLI
                     FHGFVVVVGTLILAGAVNTAIVGSNGVLNRVSEDGVLSAWFRQPHKRFGTSHRIINIV
                     VALQVITIIISRGDVTFLANLYAFGVIWSFAMNGLAVLVLRYTSPGHRDFQVPFNLTI
                     RGVQIPVGLGLITLTLFLIAVVNLFTKPIATMAGGAFSVLLYMVFTISERRGKRGGAA
                     HVEMDQFNLELESELTPEAVGARPGNILVPISNHYALYHLGNVLDRIKPGRRDLVVLH
                     VRLLRRSASGENELEADQLFGSIEQQLFSQALSMAEKRGKSIRLAVVAANDLWDGILR
                     AATSLHSSTIVAGRSTKESNEEQARQIGEAWEKLGDQKPQFNLEIHLPTGEKLYKVLG
                     PHAPNLTANEVNLLHRLWLRFSDAMAPLEMHHHDVVHFALEEVQKEVDEGHEDLVVNR
                     LRAHLEENQKKRKPSA"
     gene            complement(3103..3492)
                     /locus_tag="VGR03_00295"
     CDS             complement(3103..3492)
                     /locus_tag="VGR03_00295"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEV1992750.1"
                     /translation="MPTPPDPRKKPTSIKVHVSSGTGVDVTWADGHASHYDFAYLREE
                     CPCATCNDAREKKASLGAAGPALNSSPLLPMFKPKPRAQAATQVGNYAIQISFTDGHS
                     TGIYSYDHLRNICPCAECAKAFRASEG"
     gene            complement(3508..4713)
                     /locus_tag="VGR03_00300"
     CDS             complement(3508..4713)
                     /locus_tag="VGR03_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1992751.1"
                     /translation="MSSLAPTLPRTAAKQVVIENLIKQYGTLRALGGVSFDVHTGEWV
                     ALMGPSGSGKTTLINILGGLDTLTSGHVVVDGVDLSKLRENELVRYRAEKVGFVFQQF
                     HLVPYLTAVENVMLAQYFHSVTDEKQAEEALKRVGLGDRLTHLPAQLSGGEQQRVAIA
                     RALINQPKIILADEPTGNLDEANENIVIQIFRELHNAGHTILMVTHDPDIARQADRRI
                     ELAHGHLHFDTLQHGPGHPLNCPLADTADCCTPISTDDEIRFDHLLEQIWVCGEEGKP
                     AQPERLHATGPGGKLPAVSEEPAARVLSRMTDLRLVQMQDGEVQLTPVGSQRARDVVR
                     RHRLAERLFKDTFSIEDSEAHTQACKFEHIISPELDQRICTFLGHPKTCPHGNPIPPG
                     DCCDGKSKG"
     gene            complement(4710..5861)
                     /locus_tag="VGR03_00305"
     CDS             complement(4710..5861)
                     /locus_tag="VGR03_00305"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1992752.1"
                     /translation="MFWRIIRRLFSANRGRLFVMLLALGAGAAVTAALLNLQVDAKRR
                     LTTEFRAFGPNVLVLPPNSSQFRTETLPESIVHSVPVNLQSGRVVVSSRLYLVARLSP
                     AGPGESFSAVVAGTNPLAFLAAAVSSLPNASFTSNVVPPITGHECVVGTNVARQLNAQ
                     QDADFALTSADRREICHVASFYQTGGPEDDRVAIGLRAAQRLADLPGRISAIELIVPG
                     TPSEVQTYIKELQRVLPDADVRPIRQFTEAQAKIYDRISGLLTATVGIVLLLTALCVM
                     AAMTNVAMERRNDVGLMKAIGGSMRRVLRLFLAEAALLGLAGGMIGAAAGIFLSIGLG
                     KAVFGVAARPRLIVYPVAVALTVIVAILSAFPLRRLASIRPASVFRGEA"
     gene            complement(5891..7198)
                     /locus_tag="VGR03_00310"
     CDS             complement(5891..7198)
                     /locus_tag="VGR03_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1992753.1"
                     /translation="MFLRLVADSFGRRPRHKLLTGAALALGMGVATAALSVSLDVGDR
                     LAKEFRSLGANLLVTPQADSLPLEIGGVDYRPVNAGAYLPEADLPKLKTIFWHNNIMG
                     FSPILEVPIEAWSPASKTSLTPSVAGNATLIGTWAQHTVAIPDGTTYVSGVEKTNPWW
                     NVEGKWFGESSRQCVTGINFSKKFDVKIGDDLNVGVGDHSHWITMRVVGLLSSGGPED
                     ESVLAPLSVAQELANKPGQYRKLYVSALTKPEDTFARKDPKTMTPVEFDRWYCTPYIS
                     SIAFQIQQELPGTDVRVIRRVAEGEGAILTRVRMLLWLVTGAALLAAALAVGASSAAS
                     VIERRSEIGLMKALGAGSGTVGFLLAAEQLLLALVGGGAGYAIGIILARLLGERIFGA
                     APEPSLIVFFVIIALAAAVTILGSALPLHRASRYEPAPILRGE"
     gene            complement(7242..8504)
                     /locus_tag="VGR03_00315"
     CDS             complement(7242..8504)
                     /locus_tag="VGR03_00315"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing protein"
                     /protein_id="HEV1992754.1"
                     /translation="MLSALLIALREGVEASLVVGIILVYLSRTGRGHLARFAWYGVAA
                     AAALSLGVAVALERWRISEDGFEGLMLLVAAVFVVTMIVWMNRVARHLKKEIEAKVEA
                     YAEKAGKAAGWGIFLFVFLMVLREGAELALILRAVELSSEGLQTWIGTIAGIAGAVAV
                     GLFFFKGTLRIPLHRFFAATTVILMLVAFQLAITGLHELSEARWLPSSKAEMAILGPI
                     VRNELFFFVFIFGAAALLVLREWQAASHAKLAKETLNDAEKRLLESQNRRQRRWMIAA
                     ATASLTVILVLTADFIYVRANSAPPTARPIDAAGDIVRVAVSEVQDGSLHLFTVNAGG
                     QLLRFMIIKKPNGWGVALDACRICGAEGYRQDGQNVICRHCASAIYIPSIGDQGGCNP
                     IGVPSRMDGGDLVIDISALTQAAKEIPQ"
     gene            8630..>11130
                     /locus_tag="VGR03_00320"
     CDS             8630..>11130
                     /locus_tag="VGR03_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEV1992755.1"
                     /translation="MSTFRQSASLLLSLLLCAQVLAQAPPASQLQTAMVHPDPKRAQK
                     AIERGEKDEAAGRFDEALVAYQEAARYAPQEAYVLERGAALRSKLVRAYVEAAERDAL
                     AGLLTHATEELGMALQIDPGNTIVQERLAQLRAMEDEPRAKPSTGISGLPRLQPQAGK
                     QNLNLRGDTKTVYEQLASLFGVKATYDPDLTVRNVRLRVDDVDFYTGVSLLGTQSATF
                     WRPLNPTLMFVAPDTPEKRRQYGLEAEQTFPLSAAVGPEDVTEMLRVLRDITGATRID
                     IDTRSRTITMRDTPERLALAGELIRQLESARGEVMLEIEFLAVDRNKARALGITPPAS
                     EQLIGLNTSDLNKLKSSTNLTNLLTNIQQVFAGKGFSSIPSVIPIGGGLSTFLLTLPS
                     FAANFSDTLSLVQSGRQVLLRAQDGKPASFFVGDRFPITLSLLSGSVGNAGILTGLPG
                     STIFPQTSVAVVANPSALVANSFTGGTLPDLAVVFNNPFDPSKPKANTFAILQNQDNG
                     NFVQVKPSPITLSANETGQVAIGTGVFRNNSTFTTSQPADVVLVNSTSNNISILLGSV
                     DATTGKANGLFTEAPGSPFAVGNNPRSVIVADFNGDGFLDLAVANQGDNSISLFRGRG
                     DGTFVAFPGSPFKLTNTATISETAPVAMVSANFRNSINTINNGAEVDLAVVNQGTNNV
                     AILLSSVDSSLNVTLAEAPNSPIPVGTTPVAIATGDLNADGVPDLAVVNQGVNTVSIL
                     LGSSNLDGTFTQATGSPLSTSPTPAGIVIANFANGKVPDLAVTNSDLRTNTVGVYLGL
                     GKGTFSSRLELTTPPGPTALIASTLTTSGLPDIALV"
BASE COUNT         2308 a         3399 c         3239 g         2184 t
ORIGIN      
        1 ccctcacgaa gcttcgcatc gaacgcgcca cttaagcgga cgccatcgcc atggtcacgc
       61 ccgagcgcct actccgattc acaatcgaat tgatcttcgt cttgctcggc tttctggtga
      121 tctggctggg cttggcgcgg cacatttatg tggatcggca cagtgcctcc tggttgattt
      181 tcggcgcggc gctgatcttg tggggtctgc gggcgctgta caagccgggc cagtggtggt
      241 tgcgctggca gaactggacg cgcgggcttt cgctcgctct tctgggcgtc ctcatgctcg
      301 tgatttcacg cgtaccattc ctctgggttg gtccgatgct ggcggccggc ggcggcttgc
      361 tcgtactgcg cggcatgatc gccgcgttct tgatcttccg gccgcgctaa agaagcgcct
      421 tccagagggg cgaggcggca aatcctacga aacgctgaag accgcgaagg acgcaggcgt
      481 ccctaccctg aattacggga tctttctgaa ttcggtgatc aattttctga tcgtggcctt
      541 tgccgtattc cttgtcgtgc gcaggtaaac cgctggacca agaagccgga cgcccccgcc
      601 gcgcctacga cgaaagactg ccccccagtg cgcaatgacg attccgctag cggcgaagcg
      661 ctgcggccat tgcacggcgc agctcgctta agcacaccca agaatctttg agagatagga
      721 gtgagcgttc gccttcgtcc taggctgacg gcttgcgctt tttttgattt tcttcgagat
      781 gcgcgcgcag gcggttgacc accaagtctt cgtgtccttc gtccacctct ttttgcactt
      841 cttccagcgc gaagtgcacg acgtcgtgat ggtgcatttc caggggggcc atggcgtcgc
      901 tgaaacgcag ccacaagcgg tgcagaagat tgacctcgtt ggccgtcaga ttcggcgcgt
      961 ggggcccgag gaccttgtaa agcttctccc cagtgggcag gtggatttcc agattgaatt
     1021 gcggcttctg atcgccaagt ttttcccagg cttcaccgat ctgccgcgcc tgctcctcgt
     1081 tcgattcctt tgtcgaacgc cccgcaacaa tcgtgctgga atggagtgag gtcgcggcac
     1141 gcaggatgcc atcccacaaa tcgttcgcgg ccacgacggc gaggcggatc gacttgcctc
     1201 gtttttcggc catcgaaagc gcctgagaga agagctgttg ctcgatgctg ccgaacaact
     1261 gatcggcctc gagttcgttt tcgcccgaag ccgagcggcg caagagccgt acatggagca
     1321 cgacaaggtc gcggcgcccg ggcttaatgc gatccagcac gtttccaaga tgatagagcg
     1381 cgtagtggtt gctgatcggg acgaggatgt tgccggggcg cgcacccacg gcctcgggag
     1441 tcaattccga ttccaactcg agattgaact gatccatctc gacgtgagct gcgccgccgc
     1501 gtttgccccg gcgttccgaa atcgtgaaca ccatgtagag gagaacggag aaggcaccgc
     1561 cagccatcgt ggcgatgggc ttggtgaaca ggttcacgac ggcgatcaag aacagcgtca
     1621 aggtgatcaa cccaagtcct acgggaattt gcacgccgcg aatcgtgaga ttgaacggga
     1681 cctgaaagtc ccggtggccg ggactggtgt aacgaaggac gagcactgcc aggccgttca
     1741 tcgcgaagct ccagatcacg ccgaaagcgt agaggttcgc gagaaaggtg acgtcgccgc
     1801 ggctgatgat gatagtgatg acctgcagcg ccacgacgat attgatgatg cgatggctcg
     1861 tgccgaaccg cttgtgcggc tgccggaacc atgcgctgag tacgccgtct tcggaaacgc
     1921 ggttcagcac gccattcgag cccacgatgg ccgtgtttac cgcgcccgcc aggatcaagg
     1981 tgcccaccac gacaacaaag ccgtggaata tcagacgcgc gaaaaatggc ccttcgagat
     2041 tcatcgccag cccgccgatc aggttctcaa ggaagtgctg gcggacgtca tccggaatga
     2101 tcatcaccgc aaagaaggag accaaagagg taaagaccaa actgtagata aatatgaccg
     2161 ctccggcctt tttcaggttg gggagcttgg ggctttcgat ttcgcgatag acctgcgcaa
     2221 gcgattcctc gccgctcatc gccaggaccg agtggccgag ggcgatgaag atggcgatga
     2281 taccgaacat gtgaggaagg ctgctgtgcg cgagccagcc gagggcgctg gtgctcagcc
     2341 tgaggttgcg cggttgtggc cacggaggca gcgcggaatg gcgcaccgcc accgtgtaga
     2401 cacaccaggc gatgagcatc actaccatca cggtgaccag cttcatgata tgcagagctt
     2461 tttcgctgga ctcggcaacg cccttggtat tctcccacca gaaataaagc gtgacgacaa
     2521 tcgcaaagct cgccgcgatg aaactgattg ttccggaaga gagcgcgcca ctcacgtgaa
     2581 aatactgcag caggtcgttg atcaatcccg cgagatactg gcccgcggcg acgccgctga
     2641 tcggccccgt gaggatgtag tcaaacatga gcgcggaaac gctgaatttc gcgagcgtcc
     2701 cgccgaggga catcttcacg gctcgataca cgccgccgcg cacgaacatg ctgcagcttt
     2761 ccacgtaaac cgcgcgcacg gcgtacgaaa acagcatgat ggcgaggatg aaccacggcg
     2821 cggtcttgcc gatgtagcct tccgcctcgc cgccggcgta gtacgccgag gaggccaggt
     2881 cgttcagcac gatggccgcc gcccgccaga acgaaatgaa tgtcaggagg gctgtggtgg
     2941 cgacgatgac acgcggaata ccgctgcccc ggccgcctgc ggcagcagag ctattgggag
     3001 tgctgctcat gtgtgggttt cgtcaactcg taggttcaaa tgctcgcatt ttttcggggc
     3061 taagtctagc agttggacac tccggtgaat cggcaaaatg ctttagcctt ccgaagcgcg
     3121 gaaggctttg gcgcattcgg cgcaagggca gatgttgcgg agatggtcgt agctgtagat
     3181 gccggtagag tgcccgtcgg tgaagctgat ttgaatggcg tagttgccca cctgcgtggc
     3241 ggcctgggcg cgcggcttgg gcttaaacat gggcagcaga ggagatgaat taagcgccgg
     3301 acctgccgca ccaagcgagg ctttcttctc tcgcgcgtcg ttgcaggtgg cgcagggaca
     3361 ttcctcgcgc aaataagcaa aatcgtagtg gctcgcgtgg ccgtcggccc aggtgacatc
     3421 cacacccgtg ccgcttgaaa cgtgaacctt tatgctcgtt ggtttttttc gcgggtcggg
     3481 aggagtcggc atggtttgga tctgcgatca gcccttggac ttgccgtcgc agcagtcgcc
     3541 cggcggaatg ggattcccgt gtgggcaggt cttcgggtgg ccgagaaacg tgcaaatgcg
     3601 ctggtccagt tccgggctga tgatgtgttc gaacttgcag gcttgcgtgt gcgcctcaga
     3661 atcttcaatc gaaaaagtgt ccttgaagag acgctcggcc aggcggtgac gccggacaac
     3721 gtcgcgtgcc ctctggctgc ccacgggcgt gagttgcact tcgccgtctt gcatttgcac
     3781 caggcgcaga tcggtcatgc gcgacagaac tcgcgcggcg ggctcctccg ataccgcagg
     3841 aagcttccct ccagggccgg tcgcgtgcag gcgctccggc tgggccggct tcccttcctc
     3901 gccgcagacc caaatctgct caaggagatg gtcgaaacgg atttcgtcat cggtggaaat
     3961 gggtgtgcaa cagtccgcgg tgtcggcgag cgggcaattg agcggatggc ccggaccatg
     4021 ctgcaacgta tcgaagtgca gatggccgtg ggcaagttca atgcggcggt cagcctggcg
     4081 cgcgatgtcc gggtcgtgcg tcaccatcag gatggtgtgc cccgcattgt gcagttcgcg
     4141 gaaaatctgg atgacgatat tttcgttggc ctcgtcgaga tttccggtgg gctcgtcggc
     4201 aagaataatt ttcggctgat tgatcagcgc gcgcgcgatc gccacgcgct gctgttcgcc
     4261 gccggagagt tgcgcgggga gatgcgtcag gcggtcgcca aggcccacgc gctttagggc
     4321 ttcttcggcc tgcttttcgt ctgtgacgct atggaaatac tgggcgagca tcacgttctc
     4381 aaccgccgtg agatacggca ccagatgaaa ttgctggaag acgaagccga ctttttccgc
     4441 gcggtatcgg actagctcgt tttcacgaag cttcgaaaga tcgacgccat caacgaccac
     4501 atgtcctgag gtgagcgtgt ccaggccgcc tagaatgttg atcagcgtgg tttttccaga
     4561 gccagaaggc cccatgagcg cgacccattc gccggtgtgc acatcgaagc tgacgccgcc
     4621 gagcgcgcgc agcgtgccgt attgcttgat caaattttcg atcacgactt gctttgccgc
     4681 ggttcgcggc agcgttggag ccagggaact cacgcttcac ccctgaaaac cgatgccggg
     4741 cggatgctgg ctagccgccg cagcgggaat gcgctaagaa tcgcgacgat cacggtcaac
     4801 gccactgcta caggatacac aatcagccgc ggccgcgccg ccacgccaaa gaccgctttg
     4861 cccaaaccga ttgacaaaaa aatccccgcc gccgcgccga tcatgccgcc cgcaagtcca
     4921 agcaaagccg cctccgccag gaaaagccgc agcacgcggc gcatcgagcc gccgatggcc
     4981 ttcatcaagc caacgtcgtt cctccgctcc atcgccacgt tggtcatcgc cgccatcacg
     5041 cacagtgccg taagcagcaa cacgatcccc accgtcgccg tcagcaaccc gctgatgcgg
     5101 tcgtagatct tggcttgcgc ttcggtgaat tggcggatgg gacggacgtc ggcgtcagga
     5161 agaacccgct gaagttcctt aatataggtt tgcacttcgc tgggagtacc tgggacgatg
     5221 agctcgatgg cgctgatgcg gccgggaaga tcggccagcc gctgcgcggc gcgcaatccg
     5281 atggcaaccc ggtcgtcttc cggtccgccg gtttggtaga agcttgcgac gtggcagatc
     5341 tcgcggcgat ctgcactagt caaggcgaaa tctgcgtctt gttgggcgtt caactgacgc
     5401 gcaacattcg tgcccacaac gcactcgtga cccgtgattg gcggaaccac attggaagtg
     5461 aaagaggcgt tgggcaaaga gctaaccgcg gctgcgagaa acgctagcgg attggtaccg
     5521 gcgacaactg cagaaaatga ttctccgggt cctgcaggag aaagccgggc taccaggtag
     5581 agacgggaag atacgacgac gcggccgctt tggagattca ccggcacaga atggacaatg
     5641 gattcgggca atgtctcggt gcggaactga gaagaatttg gcgggaggac gagtacgttg
     5701 ggaccgaagg cgcggaattc ggtggtgagg cggcgcttgg cgtcgacttg aagatttagg
     5761 agcgcggccg tgacggcagc gcctgcgccg agggccaaaa gcatcacgaa gagcctgccg
     5821 cgattcgcgc tgaacaggcg tcggatgatg cgccagaaca tggccctatt tgttgcgcga
     5881 gagcctggca tcattcgcct cgcaggatag gcgctggctc gtagcgtgaa gcacggtgca
     5941 acggaagggc actgccaaga atcgtgacgg cggcagcgag tgcaatgatc acgaaaaaga
     6001 cgatgagcga aggctcaggg gccgcgccaa agattctttc tccaagaagg cgcgccagaa
     6061 taatcccgat tgcataaccg gctcccccgc ctacaagcgc gagcaaaagc tgttctgctg
     6121 caagcagaaa tccaacggtg ccggagcccg cgcccagcgc tttcatcaag ccgatctccg
     6181 agcgccgctc gatcaccgaa gccgccgaag acgcccccac tgccaacgct gccgccagca
     6241 gcgccgcgcc cgtaaccagc cacagcagca tgcgcacgcg cgtaagaatc gcgccttcgc
     6301 cttccgccac gcggcgaatc acccgaacat cggttccagg caattcctgc tgaatttgaa
     6361 acgcgatcga ggaaatatag ggcgtgcaat accagcggtc aaattccacc ggcgtcatgg
     6421 tcttggggtc ttttcgcgca aaggtatcct ccggtttcgt cagcgcgctc acatacaact
     6481 tgcggtactg tcccggcttg ttggcaagtt cctgtgcaac ggaaagtggg gcgagtacac
     6541 tttcatcttc tggtcctccc gatgaaagaa gacccactac tctcatggtg atccagtggc
     6601 tatggtcacc aacaccaacg tttaaatcgt cgccgatctt tacgtcaaac ttctttgaga
     6661 aattgatccc cgtaacacat tgccgagaac tctcgccaaa ccactttcct tcgacattcc
     6721 accaggggtt ggttttctcg actccgctga cgtaggtagt gccgtccgga atggcgacgg
     6781 tgtgctgggc ccaagttcca atcagcgtcg catttccagc gacggacgga gtgagcgaag
     6841 tcttggaagc cggcgaccaa gcctcaatgg ggacctcgag gattggactg aagcccatga
     6901 tgttgttgtg ccagaaaatc gttttcagct tcggcaagtc cgcttccggc agataagcgc
     6961 ctgcattgac gggacgataa tccacgccgc cgatttcgag cggcaatgaa tccgcttgcg
     7021 gcgtcaccag caaattcgcg cccaagctcc ggaattcctt cgccaatcga tcgcccacat
     7081 ccagcgaaac gctcagcgcc gccgtggcaa ctcccatgcc gagagccaac gccgccccgg
     7141 tcaggagctt gtggcgcgga cggcgtccaa aagaatcggc aacgagtcgc aggaacataa
     7201 gctagttggt agcgcaaatg agggcacgaa ccgtcgtgcc gctactgagg aatctccttt
     7261 gcagcttgcg tcaacgccga aatatcgatc actaagtccc cgccatccat gcgcgatggc
     7321 acgccgatcg ggttgcaacc cccctgatcg ccaatcgacg gaatgtaaat cgccgaagcg
     7381 cagtgccggc aaatgacgtt ctgtccatcc tgccggtagc cttccgcgcc gcagatgcgg
     7441 caggcatcca gcgccactcc ccatccgttc ggcttcttaa tgatcatgaa tcgcagcaac
     7501 tgcccgcccg cattcacggt gaaaagatgc aggcttccat cctgtacttc gctgaccgcc
     7561 acgcgcacga tatctcccgc ggcgtcgatc ggccgggccg tcggaggggc cgagtttgcc
     7621 cgcacataga tgaaatccgc tgtcagcacc agaatcacag tcagcgaagc cgtcgccgcc
     7681 gcaatcatcc atcgccgctg ccgccggttc tgcgattcca gcagccgctt ctctgcgtcg
     7741 ttcagggtct ctttcgccag tttggcatgc gaagccgcct gccattcgcg caaaaccaac
     7801 agcgccgccg cgccgaagat gaacacaaaa aagaacagtt cattccggac aatcggtccc
     7861 agaatcgcca tctccgcctt gctcgaaggc agccagcgcg cttcgctcaa ttcatgcagc
     7921 ccggtgatcg ccagttgaaa cgccaccagc atcaggatca cggtcgtcgc ggcaaagaac
     7981 cggtgcagcg gaatgcgcag cgtacccttg aagaaaaaaa gccccacggc gacggccccg
     8041 gcaatccccg caatcgttcc aatccacgtc tgcaggcctt cgctggaaag ctccaccgcc
     8101 cgcagaatca gcgccagctc cgccccctcg cgcaggacca tcaagaaaac aaaaagaaaa
     8161 atcccccagc ctgccgcctt tcccgccttc tccgcgtagg cctccacctt ggcttcgatc
     8221 tcttttttca ggtggcgcgc aacgcggttc atccatacga tcatcgtaac aacgaagacc
     8281 gccgcgacca ggagcatcag cccctcgaag ccgtcttcgc tgatgcgcca gcgctccagc
     8341 gctacggcta ctcccaggct aagcgcggcg gccgctgcca cgccgtacca ggcgaaacgt
     8401 gccaggtgcc cgcggcccgt gcgggaaaga tagacgagga taatgccgac gacgagagaa
     8461 gcttccacgc cttcacgaag tgcgataaga agagccgaga gcacgaaaac tccagttgtg
     8521 aatcacttgc aactacgatt taagtgtacc gcaaccaggg acttccgtca accggaccaa
     8581 cttggtccga tttctccagg gcacggttag gttatcctgt ttgatgctta tgagcacctt
     8641 ccgccaaagc gcctccctgc tcctgtctct gctgctttgc gcgcaagttc tcgcgcaggc
     8701 gcccccggcg tcgcagctcc aaaccgccat ggtgcacccc gatcccaaac gtgcacagaa
     8761 agctattgag cggggcgaaa aggacgaagc agcggggcgc ttcgatgagg cgctggtagc
     8821 ctaccaagaa gccgcgcggt acgccccgca ggaggcctac gtcctcgaac ggggtgcggc
     8881 gctgcgctcc aagctggttc gcgcctacgt cgaggctgcc gaacgggatg ccctggcggg
     8941 cctcttgacc catgccaccg aggaactagg aatggcactg cagatcgatc ccggaaacac
     9001 gatcgtccag gagcgtctcg ctcaactcag ggccatggag gatgagcccc gcgcaaagcc
     9061 ttcgactgga atctccggcc tgcctcggct gcagccgcaa gccggaaagc agaacctgaa
     9121 cctgcgcggc gataccaaga ccgtctacga acaactggcg agcctcttcg gcgtcaaagc
     9181 gacctatgat ccggacctca ctgtccgcaa cgtgcgcctg cgcgttgacg atgtggattt
     9241 ttacactggt gtgtccctcc tcggcacgca atcggccacc ttttggcgcc ccttgaatcc
     9301 cacgttgatg ttcgtcgctc cagacacccc ggagaagcgg cggcagtacg gcctggaagc
     9361 agagcagacc tttccactct ccgcagccgt cggcccggag gatgtcaccg agatgctgcg
     9421 cgtcctgcgc gacatcaccg gagcaacgcg catcgatatt gacacgcgca gccgcaccat
     9481 caccatgcgc gacacgcccg agcggctcgc cctggccggt gagctgatcc ggcaattgga
     9541 gagtgcccgc ggcgaggtaa tgctggagat cgaattcctc gccgtcgatc gcaacaaggc
     9601 gcgcgctctt ggaatcacac ctccggcttc cgagcagctc atcggtctga ataccagcga
     9661 cctgaataag ctcaaatcgt cgacgaacct gaccaacctg ctcaccaaca ttcaacaagt
     9721 cttcgcggga aagggattct ccagcattcc gtccgtcata cctattggtg gcggcctgtc
     9781 cacctttctg ctcacgcttc cgagcttcgc tgccaatttc tccgatactc tttcgctggt
     9841 acagagcggg cgtcaggtgc tgctgcgcgc gcaagacgga aagccagcca gctttttcgt
     9901 tggcgatcgc tttccgatta ccctctcttt gctttccgga agtgttggga acgccggaat
     9961 attgacggga ttgccgggct ccacgatttt cccgcagact agcgtcgctg tagtggccaa
    10021 tccgtctgcg ctggttgcca atagctttac cggcggcact cttcctgatt tggcggtcgt
    10081 ttttaacaat cccttcgatc ccagcaaacc caaggccaac acatttgcaa tcctgcagaa
    10141 tcaggacaat ggaaacttcg tccaggtaaa gccttcgccg atcacgctga gcgccaacga
    10201 gaccggccag gtcgccattg gcaccggagt tttccgtaac aacagtacgt tcaccaccag
    10261 ccagccagcg gacgtggttc ttgtaaattc cacttcgaat aacatttcaa ttttgttagg
    10321 aagcgtcgac gccaccactg gcaaggccaa cggacttttt acggaggctc ccggctctcc
    10381 ttttgctgtt gggaataatc caaggtccgt catcgtcgcg gacttcaacg gtgacggttt
    10441 tctggatctc gccgtggcca atcagggcga caattccatc tctcttttcc gcggacgcgg
    10501 agacggtacg tttgtggcgt ttccgggctc gcccttcaag ctcacgaata ccgccacgat
    10561 ttcagagact gctcccgtcg cgatggtcag cgcgaatttt cggaatagca tcaacaccat
    10621 caataacgga gctgaagtgg accttgccgt cgtcaatcag ggcaccaaca atgtagccat
    10681 tctgctgagt tccgttgatt cgagtttgaa tgtcactctt gccgaggccc ccaattcgcc
    10741 tatccctgtg ggaactactc ccgtggccat cgcgacggga gacttgaacg ccgacggcgt
    10801 gccggacctt gccgtagtga accagggcgt caatactgtc tcgatcttgt tgggaagtag
    10861 caatctggac ggcacattca cgcaagctac tggctctccg ctctcaacct cgccgactcc
    10921 tgctggaatt gtgatcgcca atttcgccaa cgggaaggtg cccgaccttg ccgtgactaa
    10981 tagcgatctc cgcaccaaca cggtcggcgt ttatctcggt ctgggaaaag gcaccttttc
    11041 ctcacgcctg gaactcacca ctcctccggg gcctacagca ctcatcgcgt ccacgttgac
    11101 gaccagcgga cttcccgaca tcgctttggt
//
LOCUS       DASYVO010000007         7316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_203, whole genome shotgun sequence.
ACCESSION   DASYVO010000007 DASYVO010000000
VERSION     DASYVO010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..2467)
                     /locus_tag="VGR03_00325"
     CDS             complement(38..2467)
                     /locus_tag="VGR03_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEV1992756.1"
                     /translation="MGTVFFAAWCLLAPAVFPATEKSPHELYDAIKALRIDPSNVYRI
                     APAHHIQLRRGDAVLSFEEGTLAFFSPLDGQITGAVFSGRGHVLAAPREPVEKQQMGR
                     FLGAPVLDQEFTNAYLRFTDDTADELLRQFRAANLAAQTDASVGTQWNSAVTQLNPSY
                     TLRILFDRLSPNPKPCFYAGLEGAATGPFDVVLDAQRDEQFFLGQVHKASGGTFYDVW
                     TSHRVPDSPVLPVAFRALHYSIETTISSNNALEAAANVRLRAETGTERVLAFQLSRAL
                     TIDSVTGERGESLAFFQNEGMSLQERSARGNDYLNVVLPQTPAGHQEFTLHFHYRGNV
                     IQDAGNGVLFVGARESWYPHLGDSAEFSSYDFLLRWPRKLRLIATGSKLEEHEEGEFR
                     VGHWKTEKPMSVAGFNLGEYASASVASGSLSIDVYANRQLEEDLRKRLASHDADTLSL
                     PSGRFGSASTGHSVMPPAPTPSPADALKQLGKEIDSSIRFYETFSGPFPFHNLSVSQI
                     PGTFGQGWPGLLYVSTYSFLPSEAQRRAGLSTAGQEHFTELVPYHEVAHQWWGNVVAW
                     SSYRDQWIDEAISNYLAVLFADTKKNPDHTLHVWLARYRRQLVEKLPGATDAAGDIGA
                     LTLSTRLNSSKSPEAFEEIIYSKGSWIIHMLREMLRQPGARNPDARFTALLQTLVTKY
                     AYRALSTDDLQREVEAVMTPGMDLEGGRSMEWFFQEWVRGTGIPHYRVEFTAHHTEKG
                     YQIRGKLFQTGVPRSFIASVPLYAGGAGHGALLGTVVAAGPETSFHFVAPNLPRKIVV
                     DPQMTLLCTSE"
     gene            2561..4015
                     /locus_tag="VGR03_00330"
     CDS             2561..4015
                     /locus_tag="VGR03_00330"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1992757.1"
                     /translation="MIAASRIRAVLLGVALLFMNGRAAADTIVLKNGRRIIALSAVEE
                     GGKVKYLTSAGELSLPKSIVDHIEKGGSVPMAGSPGADAANLDIAPPAAQPIGGGDSD
                     AIERAAVHDGAIDRPYIAKLEGEARAGTKHANFNAAMGHHAAALFEISHGDMEHALAD
                     ERTALIYAPEESALLMNVAYVHLRRSEYKQSLDYLERARRVAPDNPEVAKLAGWAYYG
                     LNKMDQAVAEWKKALALRPDAEVQAALDKAQRDKKEEENYKENESSHFTLRYSGVAAP
                     ALARDVLRTLEMHFSAIESELNYSPPDSIGVILYTQEAFADITKAPGWVGALNDGRIR
                     VPVQGLSGVDQELSRVLKHELTHSFVQQKTHGHAPTWIQEGLAQWMEGQRSGENAVVL
                     AQIYSDGHAAPLGRLEGSWMGMGGDTARYAYAWALANIEYIVQANGMGDIERILDRIG
                     AGMATEAALKEVLHSGYDDVMQSTAEYLRKNYGR"
     gene            complement(4084..5241)
                     /locus_tag="VGR03_00335"
     CDS             complement(4084..5241)
                     /locus_tag="VGR03_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEV1992758.1"
                     /translation="MPDSHLPKKFLRLLKPRLPQMLATLRRFVTAESPSLEKAAADRC
                     SGVIAEEWSKHGARVERITQKHRGDLLRITHAPGRSRSTGQLLILGHYDTVYSTGTLA
                     KMPFRLESGKAYGPGTFDMKAGIVQALFALQALQHAEIRLRKRIVFLWTSDEEIGSES
                     SRKFFETEARRSDAVFVLEPSFGPRGLLKTARKGVGEAELIVHGRASHAGLAPQEGIN
                     AIHELARQLARIEKWNDLRRGVTINAGIIEGGTRTNVIPERARAVLDLRALRVSDMRS
                     LEDRLHDLRPLQTGARLEITGGFDRPPLERKMSAALFARAQLLAKEMNLSLGECTVGG
                     GSDGNYTGALGIPTLDGLGAVGDGAHSSREHVLINTMPARAALLAALLATT"
     gene            5468..5935
                     /gene="fabZ"
                     /locus_tag="VGR03_00340"
     CDS             5468..5935
                     /gene="fabZ"
                     /locus_tag="VGR03_00340"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV1992759.1"
                     /translation="MTLDIVEIQKLLPHRYPFLMVDAIVEVERLKRIVGIKNVSINEY
                     YFQGHFPGRPVMPGVLILEAMAQTGGLLLLLDIPDRDNKLLFFVAVDGARFRRPVVPG
                     DQLRIEVNVLAWRGDFCKLEGKATVNGQLAAEGTVMCKMIDREPPAKAEELAK"
     gene            5932..6717
                     /gene="lpxA"
                     /locus_tag="VGR03_00345"
     CDS             5932..6717
                     /gene="lpxA"
                     /locus_tag="VGR03_00345"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEV1992760.1"
                     /translation="MTMREIHAQAVVAASAKLGEGVKIGAYAVVGEDVELGEGCVLHA
                     HATVGGPSKFGKNNVFYSFSAIGGDPQDYTFRGERTELVAGDGNIFREYVTVSRGTQK
                     GGAKTTLGDGNFFLAYSHVGHDCLIGSNTLFVNGATLAGHVTVEDFATIGAFCPVHQF
                     CRIGRYAYIGASTVITQDVPPFSKIVTERETKSFGINAIGLERKGFSPERLQALKRAY
                     RLLVRSKLNTSQALAEMRNSLGDSADVQELIKFIESAERGIVK"
     gene            6736..>7316
                     /gene="lpxI"
                     /locus_tag="VGR03_00350"
     CDS             6736..>7316
                     /gene="lpxI"
                     /locus_tag="VGR03_00350"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEV1992761.1"
                     /translation="MPDANTNTTGWGLIAGNGRFPFLVLEGARSQGIDMAVIALKEEA
                     SAELEKITKRLHWVSLGELSKTIDLMRQEGVKQAVMAGQVKHNKIFSAIRPDWKLAKL
                     LFSLPRKNTDALIGAIARLLEEEGIHLVDSTLFLKPLVPDPGVLTRRAPNEHEAEDIA
                     YGLGVARQIASMDIGQTVVIADRACVAVEAMEGT"
BASE COUNT         1570 a         1965 c         2398 g         1383 t
ORIGIN      
        1 ccttttgtta ctgaattcgg atcggcaacg aattcggtta ttccgaggtg cagagtagcg
       61 tcatctgggg atcaaccacg attttgcgcg gaagattcgg cgcgacgaag tgaaacgaag
      121 tctccggccc ggccgctacg accgtgccga ggagcgcgcc gtgcccggca ccaccggcat
      181 agagcggaac gctggcgatg aacgagcgcg gcacgccggt ctgaaaaagc ttcccgcgaa
      241 tctgatagcc tttttccgtg tggtgagccg tgaattccac gcgatagtga ggaatgcccg
      301 tcccgcgcac ccactcctgg aagaaccatt ccatggagcg cccgccttcg aggtccatgc
      361 cgggcgtcat gacggcttcc acttcgcgct gcaagtcatc ggttgagagt gcgcggtaag
      421 catacttggt caccagcgtt tgcaggagag cggtgaaccg cgcgtcggga ttccttgcgc
      481 cgggctgtcg cagcatttcc cgcaacatgt gaatgatcca ggagcctttg ctgtagatga
      541 tctcttcaaa agcctcgggg gacttcgagg aattgaggcg tgtgctcagg gtgagtgcgc
      601 cgatgtcgcc cgctgcatcg gtggcgcctg gcaatttttc aacgagctgc cggcggtagc
      661 gcgccagcca gacgtgcaag gtgtggtccg ggttcttctt agtgtcggcg aagagcaccg
      721 cgagataatt cgagatcgct tcgtcaatcc actggtcgcg gtagctcgac catgcgacga
      781 cgttgcccca ccattgatgc gcgacttcgt gatagggaac gagttcggtg aagtgctcct
      841 ggccggcggt gctcagacct gcgcgccgct gggcttccga aggcaaaaag gaatacgtgg
      901 aaacgtaaag caggccgggc cagccctgcc cgaaggttcc aggaatctgc gagacgctga
      961 ggttatggaa aggaaaggga ccgctgaatg tttcgtaaaa acggatcgag gaatcgatct
     1021 ctttgccaag ttgcttgagg gcatcggccg ggctgggagt gggagcaggc ggcatgaccg
     1081 agtgccccgt cgaggcagaa ccgaatcgcc cggaagggag agagagggta tccgcgtcgt
     1141 gtgaggcgag gcgcttccgc agatcttcct caagctggcg gttagcgtag acatcaatgg
     1201 aaagagatcc ggaggcaacg gaggccgagg cgtattcgcc gagattgaag cctgcaacag
     1261 acatgggctt ctccgtcttc cagtgcccga cgcggaattc tccttcctcg tgctcttcga
     1321 gtttggagcc ggtggcgatc aagcgaagct tgcgcggcca gcgcaggagg aagtcatagg
     1381 aagaaaattc cgcgctatcc ccaaggtgcg ggtaccagct ttctctcgcg ccgacgaaca
     1441 gcactccgtt tcccgcgtcc tggatgacgt tgccccggta atgaaaatga agcgtgaatt
     1501 cctgatggcc cgccggagtc tgcggaagaa cgacattcaa gtagtcgttg ccgcgcgcgc
     1561 tgcgctcctg gaggctcatg ccctcatttt gaaaaaaggc gagagattca ccgcgctcac
     1621 cggtgacgct gtcgatcgtc agagcccgtg aaagctgaaa ggcgagaacg cgctccgttc
     1681 cggtctccgc gcgcaaacgc acgttggcgg cggcttccag cgcattgttc gaggaaatcg
     1741 tagtctcaat cgagtagtgc aatgctcgaa aggccacggg aagaacggga gagtccggaa
     1801 ctcgatgcga ggtccagaca tcgtaaaaag ttccgccgct ggctttgtga acttgtccga
     1861 ggaagaattg ctcgtcacgt tgtgcatcaa gcaccacatc gaacgggccg gtggccgcgc
     1921 cttcaagccc ggcgtaaaaa cagggcttag gattggggga cagccggtca aagagaatgc
     1981 gaagggtgta gctgggattg agctgagtta cggccgaatt ccattgcgtc ccgacagaag
     2041 catccgtctg agcggcaagg tttgcggcgc ggaattggcg aagaagttcg tccgcggtat
     2101 cgtccgtgaa gcggagataa gcgttggtga attcctgatc gaggacggga gcgccgagaa
     2161 aacgccccat ctgctgtttt tcgaccggtt cgcgcggggc cgccagaacg tgaccgcgcc
     2221 cggaaaaaac ggcgccggta atctggccat cgagcgggga gaaaaaagcg agcgtgccct
     2281 cttcaaagga gagaacggca tcgccgcggc gcagctggat gtgatgggcg ggagcgatgc
     2341 gataaacatt ggaaggatcg atgcgcaaag ccttgatcgc gtcatacaat tcatgcggtg
     2401 atttttcagt ggctggaaaa accgccggtg ccaggagaca ccacgccgcg aaaaagactg
     2461 tccccaccct catacaagaa gtgtagtcac tttcggcatg agggaacaag gcggaccgct
     2521 caaactcgcg atcacggatt aagatgggaa aataccgcgc atgattgcag ctagccgaat
     2581 tcgcgctgtc ttgctgggcg tcgcgctcct tttcatgaac ggccgagcag cggcggatac
     2641 gattgtactc aagaacggcc ggcgcatcat cgcattgagc gctgttgaag aaggcggcaa
     2701 ggtaaagtat ctgacctccg ccggcgaatt gagcctgccg aaatccatcg tcgatcatat
     2761 tgagaagggt gggtcggtgc cgatggccgg ttcgccggga gcggatgccg cgaacctgga
     2821 cattgccccg ccggctgcac aaccgatcgg cggcggtgac agcgacgcga tcgagcgcgc
     2881 cgcggtacac gatggagcga tcgaccgccc ctatatcgcc aagctcgagg gtgaagcgcg
     2941 agccggaaca aagcacgcca acttcaatgc cgcgatgggg catcacgcgg cggcattgtt
     3001 cgaaatatca cacggcgata tggaacacgc cctcgccgac gagcgcaccg cgctgatcta
     3061 cgcacccgag gagtcggcgc tactgatgaa cgtggcctac gtccatcttc ggcggagcga
     3121 atacaaacag tcgctcgatt acctggaacg cgcaaggcgt gtggctcccg acaatccaga
     3181 ggtggcaaaa ctggcaggct gggcttacta cggattgaac aagatggatc aggccgtcgc
     3241 ggaatggaag aaggcgctcg ctttgcggcc ggatgcggag gtgcaagcgg cgctggacaa
     3301 ggcgcagcgc gacaagaagg aagaagaaaa ctacaaggaa aacgagagca gccattttac
     3361 gttgcgctac agcggagtag ccgcgccggc attggcccgc gatgttctgc gcacgctgga
     3421 gatgcacttt tccgccatcg aatcggaact gaactattcg ccgccggatt ccatcggcgt
     3481 gatcttgtac acgcaagaag cgtttgcgga cataaccaag gctcccggat gggtgggcgc
     3541 gctgaatgat ggccgcattc gcgtgccggt gcagggattg agcggggtgg accaggagtt
     3601 atcgcgagtt ctgaagcacg agttgacgca cagttttgtc cagcagaaga cacacgggca
     3661 tgcacccacg tggattcaag agggattggc gcagtggatg gaagggcagc gcagcggcga
     3721 aaatgcggtg gtgctggcgc agatctacag cgacgggcac gctgctcccc tcggccggtt
     3781 ggaaggctcg tggatgggaa tgggcgggga tacggcgcgc tatgcgtacg cctgggcgct
     3841 cgcgaatatc gaatacatcg tgcaggccaa cgggatgggc gacatcgagc gcattctgga
     3901 tcgcatcggg gcgggcatgg ccaccgaggc ggcgctgaag gaagtgctgc acagcggtta
     3961 cgacgacgtg atgcaatcca cagcggaata cttgcggaag aactacgggc gctgatcact
     4021 tcttgcggat agttattctc tctcccctga acatcattga ttgccgctag cggttgcacg
     4081 gccctaggtg gtagccagaa gagcggctaa gagagcggcg cgggcgggca tggtgttgat
     4141 gaggacgtgc tcgcgcgagg aatgggcacc gtcgccgacg gcgccgaggc catcgagagt
     4201 gggaatgccg agcgcgcctg tgtaatttcc gtcggagcca ccgccgacgg tgcattcgcc
     4261 cagggagaga ttcatctcct tcgcgagcaa ttgggcgcgt gcgaagagcg ctgcgctcat
     4321 tttacgttcg agcggggggc ggtcgaaccc gccggtgatc tcaagccgtg cgccggtttg
     4381 aagcgggcgc aggtcgtgca ggcgatcttc caggctgcgc atgtcggaaa cgcgaagcgc
     4441 ccgcagatcg agaacagcgc gcgcgcgttc cggaatgacg ttggtgcgcg tgccgccttc
     4501 gatgatgccg gcgttgatgg tgacgccgcg gcggagatca ttccattttt cgatgcgtgc
     4561 gagttgccgc gccagctcgt ggatggcgtt gattccttct tgcggtgcca gacccgcgtg
     4621 cgaagcgcgg ccatgcacga tcaattcggc ttcaccgact ccctttcgcg ccgtcttcag
     4681 caggccgcgc gggccgaagg aaggttcgag gacgaaaacg gcgtcgctgc gccgcgcttc
     4741 cgtttcgaag aatttgcgcg aggattcgct gccgatctcc tcgtcagaag tccagaggaa
     4801 gacgatgcgc ttgcgaagcc gtatttccgc gtgctgaagc gcttgcagcg cgaaaagcgc
     4861 ttgaacaatt ccggccttca tgtcaaaagt tcccgggccg taggctttgc cgctttccag
     4921 ccggaaaggc attttcgcga gtgttcctgt cgagtagacc gtatcgtagt ggccgaggat
     4981 caggagttgg cccgtagagc gagatctgcc gggtgcatga gtgatgcgca acagatcgcc
     5041 gcgatgtttc tgcgtgatgc gctcaactcg cgcgccgtgc ttgctccatt cttcggcgat
     5101 gacgccactg caacgatcgg cggccgcttt ttccaggctg ggcgattccg cagtcacgaa
     5161 ccggcgcagc gtcgcgagca tctgcggcag gcgcggcttc aagagtcgca agaacttttt
     5221 ggggagatgg gaatccggca tggtgcgaag atgctatcaa tgcgccgcga tcagagcaat
     5281 cgggcaaaat tcatcacggc gaggggagaa gactcgcagt ctgatccttc gtctccgccc
     5341 ctggcaaaag accgtggtta cggagtaaga gcgcgtatcg cacagggcta gggttcgcat
     5401 ataattagaa gtttgccggg gaagagccag gcgccgctga agaggtctgc ggcgacggag
     5461 agcgcgaatg acactggaca tcgtggagat tcagaaactg ctgccgcatc gctatccatt
     5521 tctcatggtg gatgcgatcg tcgaggtgga acgcctcaag cgcatcgttg gcatcaagaa
     5581 cgtttcgatc aacgagtact actttcaggg acattttccc ggaaggcctg tcatgccggg
     5641 cgtccttatt ctggaggcca tggctcagac gggcgggttg ctgctgctgc tggatattcc
     5701 ggatcgcgac aataagctgc tcttttttgt tgctgtggac ggcgcgcgat tccggcgtcc
     5761 tgtggtgccc ggggatcaac tgcgcatcga agtcaacgtc ctggcctggc gcggtgactt
     5821 ctgcaagctg gaaggcaaag cgacggtgaa cggccaactg gctgcggaag gcacggtgat
     5881 gtgcaagatg atcgaccgcg agccgcctgc caaagccgaa gaactcgcaa aatgacaatg
     5941 cgcgaaattc acgcgcaggc agttgtggcg gcgagcgcaa agcttggcga gggcgtgaag
     6001 atcggcgcgt acgcggtggt tggcgaggac gtggaattgg gcgaaggctg cgtgttgcac
     6061 gcccacgcga ccgtgggcgg gccttcgaag tttggtaaga acaacgtgtt ttattcgttc
     6121 agcgcgatcg gcggcgaccc acaggactat acctttcgtg gcgagcgcac tgaactggtt
     6181 gcgggtgacg gcaacatttt ccgcgaatac gtcaccgtga gccgtggcac acaaaaaggc
     6241 ggcgccaaaa cgacattggg cgacggaaat ttctttctgg cgtactcgca cgtggggcac
     6301 gactgcctga ttggcagtaa cacactattt gttaatggag cgacgctggc ggggcatgtg
     6361 acggtcgaag acttcgcaac cattggggcg ttttgtccgg tgcatcaatt ttgccggatc
     6421 ggccgctacg cctacatcgg cgcatcgacg gtaattacgc aggatgtgcc gccgttttcc
     6481 aaaattgtca ccgagcgcga gacgaagagt tttggcatta atgcaattgg cctggagcgc
     6541 aaaggatttt cgccggagcg tttgcaggcc ttgaagcgcg cgtatcgcct gctcgtgcgc
     6601 tcgaaactga atacttcgca ggcgctcgcc gaaatgcgga attcgctggg cgattccgcc
     6661 gacgtgcagg aattgatcaa attcattgag agcgccgaac gcggcatcgt gaagtaactc
     6721 ctgactgctg atttcatgcc ggacgcgaat accaacacga caggatgggg actcatcgcc
     6781 ggaaacgggc gctttccgtt cctggtgctg gaaggcgcgc gcagccaggg aatcgacatg
     6841 gcggtgatcg cgctgaagga agaggcgtcc gcggagctgg aaaaaataac caaacggctg
     6901 cactgggtaa gcctcggcga gttgagcaag acaatcgact tgatgcgtca ggaaggcgtg
     6961 aagcaggcag tcatggccgg gcaggtgaaa cataacaaaa tattcagcgc catccggccg
     7021 gactggaaac tggcgaagct gctgttttct ctgccgcgga agaatacaga cgcgctgatc
     7081 ggagcaatcg ctcgcctgct ggaagaagaa ggtatccacc tagtggattc gacgctgttc
     7141 ctgaagccgc tggtgcctga tcccggcgtg cttacgcggc gggctccgaa tgagcacgaa
     7201 gcggaagaca ttgcctatgg gctcggcgtg gccagacaaa tcgcgagcat ggacatcgga
     7261 caaaccgtgg tcatcgcgga tcgcgcttgc gtagcagtgg aggcgatgga aggcac
//
LOCUS       DASYVO010000008        22588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_141123, whole genome shotgun sequence.
ACCESSION   DASYVO010000008 DASYVO010000000
VERSION     DASYVO010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 22588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22588
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141123"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VGR03_00355"
     CDS             complement(<1..568)
                     /locus_tag="VGR03_00355"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV1992762.1"
                     /translation="MTTAQAHRTAAKHQPRRIAIFGGSFDPIHNGHLFAARAADRRFN
                     FDGIHFIPASRPPHKLKQHLAPFPHRFAMVSLACTEHPHFVPSLAEAGEDFSGTQLHY
                     SVDTVRYFRHVYHGHGDRLFFVIGADAFLDIPMWKEYETLLGLCDFIIANRPGIRSEA
                     LRLVIPPDLMARPDSKIEAEQPSQVVAHL"
     gene            complement(636..1793)
                     /gene="obgE"
                     /locus_tag="VGR03_00360"
     CDS             complement(636..1793)
                     /gene="obgE"
                     /locus_tag="VGR03_00360"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV1992763.1"
                     /translation="MFVDEAKIFVKAGDGGNGCIAFRREKYVPRGGPSGGDGGHGGSI
                     YLEANINDNTLLRYRYNREFKADRGRHGEGSNCTGHSGQDMILRVPVGTLVFDDQGAT
                     IADLASPGQRVLIAHGGRGGRGNQHFAKPWHQAPREHEDGFPGEERHLRFELKLLADV
                     GLVGYPNAGKSTLISVISAARPKIASYPFTTLEPNLGVVNADGGTGGHGTELGRTFVV
                     ADLPGLIEGAHLGAGLGIRFLRHVERTRLLVHLIDTSDSNADDPVHSFEVINGELEAF
                     SASLIEKPMIVVATKLDATTDATRLEALCDFCKRRNVQFHSVSAVAGDGVKDLVRSIA
                     DALDKIPRLAPDSTLDSPPGPAENGDGGQDHHPSSAPVDRPAVATNKEEDS"
     gene            1899..2861
                     /locus_tag="VGR03_00365"
     CDS             1899..2861
                     /locus_tag="VGR03_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1992764.1"
                     /translation="MSRVLKRIALLFFAFAVVAAAALYVLYFRRTPLPPAAPHALAVP
                     VISTIPGLSACWVETAKTFSSFSFGMTAGSVLVKHPAGDLLIDTGSSSHYDQEISGFP
                     FATWLKLKSLAGQLKPKVPLPDLLRRIGEDPAKLRWVILSHVHLDHAGGLMDLPHLPV
                     LLTREELQFAADPEVQARGYVIAVHTQKFPSVATPTLRFEPAPYETFDQSADLYKDGS
                     VVVVPLRGHTPGSLGIFVNLSPTRRLFYVGDAVDDERGVEERAGKPLILRDSDNDMAL
                     ANQIVGRLSELHEKVPGLAIIPAHGRSAYKKFFPGGPLSCVSGQ"
     gene            complement(2962..3240)
                     /gene="rpmA"
                     /locus_tag="VGR03_00370"
     CDS             complement(2962..3240)
                     /gene="rpmA"
                     /locus_tag="VGR03_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV1992765.1"
                     /translation="MAHKKGGGSSTNGRDSHGQRLGVKRFGGQHVNAGTILVRQRGTK
                     HQAGENVGQGKDDTLFAMVTGIVKFEDKGRHGRFISVKPTEQAKSAAN"
     gene            complement(3297..3608)
                     /gene="rplU"
                     /locus_tag="VGR03_00375"
     CDS             complement(3297..3608)
                     /gene="rplU"
                     /locus_tag="VGR03_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV1992766.1"
                     /translation="MYAVIQTGGKQYRVAPGDTIRIERVDGKVGEKVNFGSVLAVHTD
                     DKKVLLGADAGKASVTGKIIQQGRAKKVIVLKFKKTNQYKIQRGHRQNYTAVQVSDIK
                     L"
     gene            complement(3705..4169)
                     /locus_tag="VGR03_00380"
     CDS             complement(3705..4169)
                     /locus_tag="VGR03_00380"
                     /inference="COORDINATES: protein motif:HMM:NF023123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HEV1992767.1"
                     /translation="MTCQLAQINISRLLAPLDDPQIAEFVAQLEPINALADKAPGFVW
                     RLQSGSGNATDIAYNEDPFVIVNMSVWESLETLRDFAYKSDHAKVFRDRPKWFEKTDK
                     PSYCLWWVPAGHIPTVAEGRERLEHYQKHGTTPYSFWFSQYFPLPADESVCA"
     gene            complement(4293..6653)
                     /locus_tag="VGR03_00385"
     CDS             complement(4293..6653)
                     /locus_tag="VGR03_00385"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEV1992768.1"
                     /translation="MPHSPPSKHSWALRLILLVLLGPSLPALAAPPAKDSVPFTNLHI
                     PRLENRPAIADFLTMQPSAAFAGKMLKVEGFIQRDPKDGSPISQKTEIYLGYTNQNLY
                     VVCVCFDSEPDKIRAHMVRREQINDDDQFGFVLDTFSDRKNGLFFYVNPLGVQQDGIW
                     NDFQEPDYSYDMLWNSQGKITPQGFVVWFEIPFRSLRFPRNPEQTWGIFFERDIRRSN
                     EFSFFPHISSNTQGWLTQETHADGLREISPGRNIQFVPYGSLRSFRSLDDRPSGVPHF
                     NGKSFEPRAGLDSKIVLKDALVFDATLNPDFAQVESDEPQVTVNQRFEVFFPEKRPFF
                     LENASYFATPINLVFTRRIADPDYGLRLTGKRGPWSIGAFFADDKSPGDSVAPADPLS
                     GAKAYFGVLRVNHDIGKQSTIGLIYTDRELATVPNTACTDNRSIAGSNRVGGIDAKIT
                     FSPTWYATVQALVSSTNLNNGLHKGGPGFWEYVEHSTRKVEYNALYQDTSEGFQTETG
                     FFRRPDIRRFSQFALYRFRPEGKHLVWHGPGLFTNNNWDHKGTRLEWFANANYRFSFQ
                     RQNFIGFFGNLGHERLRPSDFSALPGNRDYAHRHTGFFFQSGFFKQVSLNGEIGWGTD
                     TNFDAASMPPIFGRPFLANSSYAQIYLTLRPTGKLTVDNTYLLTRLRSLNQGPGIFDN
                     HIIRSKWNYQFTREFSLRFIGQYAAVLANPSLTSLQTTKNFNADLLFTYLLHPGTAVY
                     FGYNSNLQNLDSSLSHDSNGNLLHNPNRFLNDGRQIFVKISYLFRF"
     gene            complement(6825..7184)
                     /locus_tag="VGR03_00390"
                     /pseudo
     CDS             complement(6825..7184)
                     /locus_tag="VGR03_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7215..7610)
                     /locus_tag="VGR03_00395"
     CDS             complement(7215..7610)
                     /locus_tag="VGR03_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992769.1"
                     /translation="MRPRFAISIAFYLATNAASALSSRAQDNYEIQVYGYDQVEPHHT
                     MIELHSNFAFQGSRTSENGLFPTNHQLHETIEITHGFTDWFETGFYIFTSTQNGQGWD
                     WVGDHIRPRVRIPKKWKWPVGLSLSNEVG"
     gene            complement(7680..8708)
                     /locus_tag="VGR03_00400"
     CDS             complement(7680..8708)
                     /locus_tag="VGR03_00400"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEV1992770.1"
                     /translation="MKEAIAKYLEYLQSVKNSSPHTILNYRRDLEQFLAYLSPPGARP
                     PALTGVTHSMIREFVAHLHDHGLAKSSIARKLAALRSFFKYCVRESHLKENPARLVPT
                     PKLPKRIPSVLSAEEMNGFLNQLGGMGQGALGGRVTSKKGLLLPLASAMNLPAKRGRS
                     VQVGEGLLLRRDRALLELLYAAGLRVSELTGLNLADMEQKERILRVRGKGSKERIVPY
                     GGKAQEALEKYWPLREQLLEQTYRTSGGQGPYTEAIFLNYSGRRLTQRSVGRIVKKYV
                     RIANVNWDLHPHSMRHAFATHLLADGADLRAIQELLGHQSLSTTQKYTHASIRQLMDV
                     YDKSHPHA"
     gene            complement(8809..11514)
                     /gene="topA"
                     /locus_tag="VGR03_00405"
     CDS             complement(8809..11514)
                     /gene="topA"
                     /locus_tag="VGR03_00405"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV1992771.1"
                     /translation="MARSLVIVESPAKAKTINKYLGRNFIVKASIGHVMDLPKKTIGI
                     RLPGEEEGGKKKKKSKAKKGSKKAKEKEKPAGKLISIDDEKIFEPTLQIIAGKGKVIN
                     DLRKAAISADAVYLAGDPDREGEAISAHLAMVLSKPSKFIEQEPSVSRFKKKVATPEE
                     PAKKEDESASKKEVVAIDPKKIFRVTFNEITPKAIRAAFDHPRQVDTNLVDAQQARRV
                     LDRIVGYKVSPILWDKVRRGLSAGRVQTVALRLIVERELQIRAFVPQEYWTIHAMLDA
                     GQPPLFEAKLFKHKGEDMAVSNQESADKIVGSVSKAKWQVAGVTQKEKKRNPPPPFTT
                     SKLQQAAYNRLRYTAKRTMSLAQKLYEGVELGDEGSVALITYMRTDSVHVSNDALIQV
                     RELIPERFGSNYLPEKPNYYKSKKDAQEAHEAVRPTDAMRAPEDVRKYLAEDLFKLYQ
                     LIWQRFVASQMLPAIFDQTTIDIFAGDYTFRATGTVQKFDGYLRVYQMPASIADREDD
                     EKDDEGEGRALPQVAEGQTLRLDKIRPDQHFTEPPPRFNDATLVKELEEDGIGRPSTY
                     ASIISTIVEREYVTKDQGRFSPTMLGERVSVLLVKSFEDIFDVTFTARLEEELDEIEE
                     GKLPWREAVKEFWEKFVIDLDKAGDEMLSYKAGIPTGKKCEKCGQGELLERISRHGFF
                     LGCSRYPECDFIQDLSPELPEETGGEPKTEYCDNCGKEMAIKRGRFGTFLACTGFPDC
                     KTTRRLVQGTRIAHQPDEPLAELCTLCSNNLVKKHGRFGEFIGCSGYPKCKYTRPITM
                     GIRCPKCNEGEFVRRGSAGKGGRGRPRVFYGCSRYPDCDFTTPHMPIAEPCPKCGAPF
                     IVEKRTKIGTVRACLKEGCDWEMLVPEAQPQAQPEESPVPVAAKS"
     gene            complement(11702..12829)
                     /gene="dprA"
                     /locus_tag="VGR03_00410"
     CDS             complement(11702..12829)
                     /gene="dprA"
                     /locus_tag="VGR03_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEV1992772.1"
                     /translation="MQLDASLSWLALGLTPGLASRLSARVLRRFDSPDGVFRATLSDL
                     EACHLPAQVAQAIVKKEAFKRAEKELAGVQKIAGCSLLNWTEPEYPQTLLQIYDPPVL
                     LYVRGDPQVLNLPSIGIVGTRRPTLYGTQMAQRLGRELAARGLVIVSGLARGIDAIGH
                     QGAMEANGRAIGVLGTGIDVCYPKENKKLYEKVLERGAIISEFPLRTHPAPENFPIRN
                     RIVAGMPLGVVVVEGAQYSGSLITARLAMEFGREVFGVPGNVTQPVSFAPNQLIKQGA
                     KLVTCAADVIEELPTPIRAALLQAEQPEAEQRNLLAAAALNGSQKKLYELLSTEEPVP
                     IDDIVERSGLNSSEVLATLFDLEMKGIIRQLPGKQFSKVLL"
     gene            complement(12924..15995)
                     /locus_tag="VGR03_00415"
     CDS             complement(12924..15995)
                     /locus_tag="VGR03_00415"
                     /inference="COORDINATES: protein motif:HMM:NF004799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV1992773.1"
                     /translation="MLLEARVAQPVTEAEAVRLARDLYGLEATARALPGEYDDNFHLT
                     SADGHAFVLKVMHPARASSFIDLQCRALTHLAQRAPQLPLPRVTPNRSGELFSSIAAA
                     DGSTRLVWLLTFVNGKVLAEVRPHTPELLGDLGRFLGEMDAALQSFTHAAAHRELKWD
                     SSRAGWIRDYIKHIADSKRRALVKKFLALYEAEAVPILPRLRRSVIYGDANDHNVLVG
                     DPWPQPRTVVGVIDFGDIHHGLTASETAIAAAYGILGKEDPLQAATAIVAGYHGAFPL
                     DELELSVLYALMGARLAVSVTNSAHRKTLKPDDPYVTVSEAPAWEALERLEKIHPRFA
                     HYTFRAACGLSPVPKSEKIKKWLEASAGSAASILEIDLRTAPSVVFDLSVGSTFLGAD
                     PRASETPALTEKVFHAMQRAGASVGIGRYDEPRLLYTSLLFGASSNPTDERRTVHLGM
                     DIFVEPGAPLSAPLDGVVQVVANNSAPLDYGPVVILRHETSGGEEFFTLYGHLSKEAL
                     GSLKVGQRIARGQQFARVGSAQENGGWTPHLHFQVILDLLELGDDFPGVARASQRPVW
                     TSLSPDPNLLLGIPEDRFPAKEPALSETLAGRHALLGKNLSISYQRPLKIVRGWMQYL
                     YDDTGRAYLDVYNNVPLVGHSHPRVVQAAQAQLALLNTNTRYLHDNVNRYAERLTRLL
                     PAPLRVCFFVNSGSEANELALRLARAHTGREDIIVLEHAYHGHTNTLIDISPYKFDGP
                     GGCGKKSWVHVAPLADDYRGTYRRGDKQAGAKYARHVAEILERTQANGRGVAAYIAET
                     LPSVGGQIVFPPDYLAEVYRHVRGAGAVCIADEVQVGFGRLGTHFWGFETQGVVPDIV
                     VLGKPIGNAFPLAAVITTPEIANSFNNGMEFFSTFGGNPVACAAGLAVLDVLEEENLK
                     QHALRVGAHLIEGLKSLQTRHALVGDVRGSGLFLGIDLVLDRETREPAPLQASYVVNR
                     LRDWGVLAGTDGPHHNVIKLRPPLVFSESDADLFVKTLDAILQEDAAQHRS"
     gene            complement(16089..17009)
                     /locus_tag="VGR03_00420"
     CDS             complement(16089..17009)
                     /locus_tag="VGR03_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992774.1"
                     /translation="MSRRSLFFLISISALLALGFTSARANRHHHSVSISDGHKQPATD
                     CSDLRIRFDDQDAVMRSEERTLTKSEAPVLRIQPHSNGGVQVVGWDKETYSVTACKAA
                     AGSGDAAERILSQITMSIENGKISTKGPGDEDDWTVYLLIRTPKSASVDLETMNGPIS
                     LYDVDGKLTARAHNGPISLKNFSGDAEITAVNGPISLDGSRGSVRIHTENGPISVALE
                     GKTWSGTGLTADAKNGPVTLMVPSGYQSSFVVESTNNAPVSCKASICDNARKTWDDEH
                     RRIEYGSAPAVIRLSTVNGPVSVRDSREKL"
     gene            17446..18291
                     /locus_tag="VGR03_00425"
     CDS             17446..18291
                     /locus_tag="VGR03_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992775.1"
                     /translation="MRRPFLQVLMCVALTAEVLICGLSAQAQVTKGCSEINYVGADSS
                     NPFTAEYVTTSTMPTPAGVPKTMVLRENVARDSQGRIRFEKHGVAQPPDDRKTVTLET
                     PDGKPFTVTREEYGTLIDIFDCASGTFVRIQPGMRIVTVKEGKSEAPARRAKHAYSTP
                     YFPSPGSKTLPNVIQEELGTRDIQGIPARGLKTTTLGTEKDADWNGKPIRETELWVSD
                     DLAAQLLRIDKDLRTGNEGRSELLAIKREEPDPALFEIPKDCEVNPAMLPSQKGLGVV
                     RPAGQ"
     gene            19001..19909
                     /locus_tag="VGR03_00430"
     CDS             19001..19909
                     /locus_tag="VGR03_00430"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HEV1992776.1"
                     /translation="MSPRIVTKLRISIVQYLNTAPLVWGFTNGPLRGKYDLSFTVPSL
                     CAEELRNGNADVAIIPAIEYQRIDDLVILPDMAIASKKLVRSLLIVAKKPIEQVKSFV
                     LDRSSRSTQTLTRILCAEKWRIAPEFFEAPPDLGAMLQQADAALLIGDPALRIAVGIE
                     KESRPGTPGQTLCQAAALGIAGPEFLYVYDVVGEWRSLTGLPAVLAVWAARQDIATPD
                     VAADFLASGDFGLSRIPEITFDAARELELPQPALESYLRHNIDFSLDKENRRGLELYF
                     AHAAKLGLIPQVKPIKWATKPEAVKL"
     gene            20007..21041
                     /gene="mqnC"
                     /locus_tag="VGR03_00435"
     CDS             20007..21041
                     /gene="mqnC"
                     /locus_tag="VGR03_00435"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HEV1992777.1"
                     /translation="MGLTKQQALDMLESDDLVGLGMAAHQVRLKKNDPRVVTYQIDRN
                     INYTNFCTEYCSFCAFYRPLGAKDGYILSFEEIYKKIDEMLELGGTGILLQGGLHPDL
                     QIGYYENLLRSLKARYPQVHLHCFSAPEILCIAEVSEISIRETIQRLMDAGLQSIPGG
                     GAEILDDEVRAKIARLKCTSDDWEEMHRVAHSLGLRTTATMMFGCGEELRHRVNHLER
                     LRRIQEDTGGFTAFIPWMFATENTALGKKVQEATAVDYLKTLAVSRLYLDNVDHIQSS
                     WLTPGIKVCQIGLQFGADDVGSILIEENVVFAAGVKNRTNESELRRVISDAGFIPAQR
                     DTLYRSYVLK"
     gene            complement(21080..21697)
                     /locus_tag="VGR03_00440"
     CDS             complement(21080..21697)
                     /locus_tag="VGR03_00440"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEV1992778.1"
                     /translation="MEPKPAAAAHRANIDGGSRGNPGPASYGVVIRDGRGEIVAKLKK
                     YIGRMTNNVAEYYGLIAALDYADSHGVRAIRIESDSELLVKQMRGQYKVKSEDLRPLF
                     ERAQKMSKAFESFRIEHVYREQNREADALANEALDETEGKAPGAPPTKKPEPIGSKPE
                     PPSSKPEPRRVQARFRGGVLYLLEDVELPDGMVVDVSIHLRPKPL"
     gene            complement(21751..22527)
                     /locus_tag="VGR03_00445"
     CDS             complement(21751..22527)
                     /locus_tag="VGR03_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992779.1"
                     /translation="MHPAIPHLIELQRVDHQIALLRANLEGFPKRIREAETKLGSARA
                     EVASAKEAHAQSTAERKKFEFDGQQWKDRARKYRDQSGAVKTNEAFKALQHEIVNAEA
                     EVAKADDRQLEVMMAAEEVERRVKIAESRLKEAEQAVAAERKEIQSQGAEKKKQLETA
                     TAERERIIAPVPEELCELYARIAKRHNGTAMAEARDGQCRGCGMRVLPHILQELRTET
                     NEEVFRCESCGFILYTLEPIPIANPAVGSSSTSSAASTNS"
BASE COUNT         4656 a         6315 c         6835 g         4782 t
ORIGIN      
        1 gaaggtgcgc cacgacctgg gaaggctgct cggcttctat ttttgaatcc ggtcgagcca
       61 tcaagtccgg tggaatgacc aggcgcaggg cttccgagcg gatgccgggg cgattggcga
      121 tgatgaaatc gcaaaggccg agcagcgtct cgtattcctt ccacatgggg atatcaagga
      181 aggcgtcggc gccgatgacg aaaaagaggc gatcgccgtg gccgtggtag acgtgccgga
      241 agtagcgcac agtgtccacc gaatagtgga gttgcgtgcc gctgaaatcc tcgcctgctt
      301 cggccagcga gggaacgaag tgcggatgct cggtgcaagc gagcgagacc atggcaaaac
      361 ggtgcgggaa cggagcgaga tgctgcttca gcttgtgcgg aggacgactc gccgggatga
      421 agtggattcc gtcgaagttg aagcgccggt cggcggcgcg ggcggcgaaa agatgtccgt
      481 tgtggatggg atcgaaggaa ccgccgaaaa tcgcaatacg gcgtggttga tgttttgcgg
      541 cggtgcggtg tgcctgcgct gtggtcaatg aagctcccaa aaatgtgcag ccggcccaga
      601 ttggatcaag gccacgcaca acatatgtaa ttctctcacg aatcttcttc tttgttcgtg
      661 gcgacggccg ggcggtcaac aggcgcggaa gaaggatgat gatcttgtcc tccatccccg
      721 ttttcggcgg gacccggtgg cgagtccagg gttgaatcgg gagccaggcg aggaatcttg
      781 tccagggcat cggcgatgga gcggaccaga tctttcacgc cgtcacccgc gacagcggaa
      841 acggagtgga attgaacgtt gcggcgcttg cagaaatcgc aaagcgcctc gagccgcgtg
      901 gcatcggtcg tagcgtccag cttggtggca accacgatca tcggtttttc gatgagggag
      961 gcgctgaagg cttcgagctc gccattgatg acttcgaacg agtgcaccgg atcgtcggcg
     1021 ttggaatcgc tcgtgtcgat cagatggacg aggagacgcg tgcgttcgac gtggcggagg
     1081 aaacggatgc ccaggcccgc gcctaagtgt gcgccttcga tgaggccggg aagatcagcg
     1141 acgacaaacg tgcggcccag ttcggttccg tggccgccgg tgccgccgtc ggcgttgacg
     1201 acgcccagat tcggttccag cgtcgtaaag ggatagctgg cgattttcgg gcgcgccgcg
     1261 gagatcacgg aaatcaaggt ggacttgccg gcattggggt aacccaccag gccgacgtcg
     1321 gccagaagtt tcagctcgaa acgaagatgg cgctcttcac cgggaaatcc atcttcatgt
     1381 tcgcgcgggg cctggtgcca gggcttggca aagtgttgat tgccgcgccc accgcgtcca
     1441 ccgtgggcaa tcagtacgcg ctggccaggg ctagcgagat cggcgatagt cgcaccttga
     1501 tcgtcgaaca ccagcgtgcc gacgggaacc cgcaagatca tgtcctgacc ggaatggccg
     1561 gtgcagttgg agccttcgcc gtggcggccg cgatcggcct tgaattcgcg attgtagcgg
     1621 tagcggagca gcgtgttgtc gttaatgttg gcttcgagat agatgctgcc gccatggccg
     1681 ccgtcgccgc cggagggacc gcctcgcggc acgtattttt cgcggcggaa ggctatgcag
     1741 ccgttgccgc cgtctccggc cttcacgaat attttggctt catcaacgaa cacaagaata
     1801 tatctccgta aaagttgacc gctaggcggt cacccgtgaa aaagataaca taagcaaact
     1861 atcgcaacgc gaaatcgccg aatggaggcc gtatcccaat gtcccgcgtg ctcaaacgca
     1921 ttgccctgct atttttcgcc ttcgcagtgg tggcggctgc cgccctatac gtgctctact
     1981 tccggcgaac gccactgccc cctgcggcac cccacgcgct cgccgtcccg gtgatttcca
     2041 ccattcccgg gctttccgcg tgctgggtag aaacggcgaa aacattcagc agcttttcct
     2101 tcggcatgac tgccggaagc gtcctcgtaa agcatcccgc gggcgacctg ctgatcgaca
     2161 ccggcagctc cagccactac gaccaggaga tttccgggtt cccgtttgca acgtggctca
     2221 aactcaaatc actcgccggg cagctcaaac cgaaagtgcc gcttccggac ttgttgcgcc
     2281 gcatcggtga agatccggcg aagctgcgct gggtgatcct gtcgcacgtg cacttggatc
     2341 acgccggcgg cttgatggac ctaccgcatc tgcccgtgct gctcacgcgc gaagagctgc
     2401 aatttgcagc cgatccggaa gtgcaggcca ggggctacgt aattgccgtg cacacccaaa
     2461 agtttccgtc cgttgccacg cccactttgc gattcgaacc ggcgccctat gaaacttttg
     2521 accagagcgc cgatctttac aaagacggct cggtggtcgt cgtgccgttg cgcggccata
     2581 cgcccggaag cctgggcatt ttcgtgaacc tctcacccac gcggcgcctg ttttacgttg
     2641 gcgacgccgt tgatgacgag cgcggtgtcg aagagcgcgc cggaaaaccg ctgatcttgc
     2701 gggacagcga caatgacatg gctcttgcga accagatcgt tggcaggctc agcgagcttc
     2761 acgaaaaagt gccgggactt gcaatcattc cggcgcatgg acggagcgcg tacaagaaat
     2821 tcttccccgg tggacctttg agctgtgttt ccgggcaata gccgcgttga aaagaaaaac
     2881 acggtccagc ggggccggac cgcggaatcc agaacgggcg gactgaagtc cgcccctaca
     2941 aaatgcgacg gcaaaatgca gctagttcgc ggcggacttg gcttgctcag taggctttac
     3001 gctgatgaag cggccgtggc ggcccttgtc ttcaaacttc acaatgccgg taaccatcgc
     3061 gaagagcgtg tcgtccttgc cctgaccgac gttttctccg gcctggtgct tcgtgccgcg
     3121 ctggcgcacc agaatcgtgc cggcgttgac gtgctgccca ccgaagcgtt tcacgcccag
     3181 cctctggccg tgcgaatcgc ggccgtttgt agaggagccg ccgcctttct tatgtgccat
     3241 aacgtaatcc ttttcgagaa aaactgccgg gtggaagccc gggggaatgt gaagtgttac
     3301 agcttgatat cgctgacctg cacagccgtg tagttctgcc ggtggccgcg ttgaatcttg
     3361 tactgattgg tcttcttgaa cttgagcacg atgaccttct tggcgcgccc ctgctggatg
     3421 attttgccgg tcaccgacgc tttgccggca tcggcgccca ataagacctt cttgtcgtcc
     3481 gtatgaacgg ccaagacgga gccaaagttc accttttctc ctaccttgcc atcgacccgc
     3541 tcaatgcgga tagtgtcgcc gggcgcgacg cgatactgct ttcccccggt ctgaataacg
     3601 gcgtacatct gtcctccaag cgctaaacgc aaactctaag tatagaggtt tctgacttga
     3661 atcgtcaacg aacgaaatat tgacgcattg ggcgctcgga cgaactacgc gcagacgctt
     3721 tcgtccgcgg gcagggggaa gtactgcgaa aaccaaaagg aatacggggt cgtgccatgc
     3781 ttctggtagt gctccaaccg ctcgcggccc tcggcgacgg tggggatatg gccggctgga
     3841 acccaccaga ggcaatagct gggcttgtcc gtcttttcga accactttgg gcggtcacga
     3901 aataccttgg cgtggtccga cttgtaagcg aaatcgcgca gcgtctcgag cgactcccaa
     3961 acggacatat tcacgatcac aaaggggtcc tcgttgtacg caatatctgt ggcgttgccg
     4021 gaccccgact gcagccgcca gacaaaacca ggagccttgt cggcaagagc attgatgggt
     4081 tcgagctgcg cgacgaattc ggcgatttgc ggatcatcca gcggagcgag aagacgcgat
     4141 atgttgattt gagcgagttg gcaggtcatg ggcaagttcg attcattctt tccaaagaac
     4201 gcatcggaaa ttaaaaacgg taatacagga tggtccacat gacgaagtgg ccggtggaga
     4261 aggagatcgt ggggaagact ggaggcgtgg attcaaaagc ggaagagata ggagattttg
     4321 acgaaaatct ggcggccatc attcaagaag cgattgggat tgtggaggag gttgccgttg
     4381 gaatcatggc tgaggctgga atcgagattt tggaggttgc tgttgtaacc aaagtagacc
     4441 gcggtgccag gatggagaag ataagtgaag agcaggtcgg cgttgaagtt tttggtcgtt
     4501 tggagagaag tgagacttgg gttggcaagc actgccgcat actgaccgat aaagcgaaga
     4561 gaaaattcgc gagtgaactg gtagttccat ttcgagcgga tgatgtgatt gtcgaagatg
     4621 ccgggacctt ggttgaggct gcggaggcgg gtgagcaggt aagtattgtc caccgtgagt
     4681 tttccagtgg gacgcagcgt gagatagatc tgcgcatagc ttgaattggc aagaaaaggg
     4741 cggccaaaga tgggaggcat ggaggcggcg tcgaagttgg tgtccgtgcc ccagccaatc
     4801 tcgccgttca gggagacctg tttgaagaaa cccgactgga agaagaatcc ggtatgacgg
     4861 tgagcgtagt cgcggttgcc tgggagagcg ctgaagtcag aggggcgaag gcgctcgtga
     4921 ccaagattgc cgaagaagcc gatgaagttt tggcgctgga atgagaagcg gtaattggca
     4981 ttggcaaacc attcgagacg cgtgcccttg tggtcccagt tgttgttggt gaagaggccc
     5041 ggaccatgcc agacgaggtg cttgccttcg ggacgaaagc ggtagagagc gaactggctg
     5101 aaccggcgga tgtcggggcg gcggaagaaa cccgtctcgg tctggaaacc ctccgaagta
     5161 tcctggtata aggcgttgta ttccactttg cgcgtgctgt gctcgacgta ttcccaaaag
     5221 ccaggcccgc ccttatgtaa accgttgttg agattagtgg agctgacaag cgcctgcacg
     5281 gtggcgtacc aggtagggct gaaggtgatc tttgcgtcga tgccaccgac gcggttggag
     5341 ccggcgatgg agcgattatc agtgcaggcg gtgttgggca cggtggcaag ctcgcggtcg
     5401 gtatagatga gaccgatggt ggattgtttg ccgatatcgt gattcacgcg gagcacgccg
     5461 aaataggctt tggcgcccga gagagggtca gccggagcga cggagtcacc cggagattta
     5521 tcgtccgcga agaaggcgcc gatggaccag ggaccgcgtt tgccggtgag gcgtaagccg
     5581 tagtcgggat cagcgatgcg gcgcgtaaag acgagattta tgggagtggc gaagtagctt
     5641 gcgttttcga gaaagaaggg gcgcttttcc ggaaagaaaa cttcgaaacg ctgattgacg
     5701 gtgacttggg gctcgtcgga ttcgacctgg gcaaaatcag gattcaaggt ggcgtcaaag
     5761 accagggcgt ccttgaggac gattttggaa tcgaggccgg cgcgcggttc gaagctcttc
     5821 ccgttaaaat ggggcacgcc gctgggacga tcgtccaggc ttcggaacga gcgcagggaa
     5881 ccatagggaa caaactggat gttgcggccg ggagaaattt ctctgagtcc atccgcgtgt
     5941 gtttcctgag tgagccagcc ttgtgtattg ctgctgatgt gcgggaaaaa ggaaaattcg
     6001 ttgctgcggc ggatgtcacg ctcaaaaaag atgccccagg tctgctccgg attacgggga
     6061 aagcggagcg aacggaacgg aatttcgaac cagacgacga aaccctgtgg cgtgattttg
     6121 ccttgtgaat tccagagcat gtcgtaggaa tagtccggct cctgaaaatc gttccagatg
     6181 ccgtcctgct ggacaccgag aggattcacg tagaaaaaga ggccgttctt gcggtcgcta
     6241 aaggtatcca ggacgaagcc gaactgatcg tcgtcgttga tctgctcgcg ccggaccatg
     6301 tgagcgcgaa tcttgtccgg ttcggaatca aagcaaacgc aaacgacata gagattttga
     6361 ttggtgtagc cgagatagat ctcggttttc tgagagatgg gggaaccgtc tttgggatct
     6421 cgttggataa agccttcgac cttgagcatc ttgccggcga aggcagctga gggttgcatc
     6481 gtaagaaagt cggcgatggc cggacggttt tccaagcggg ggatgtggag atttgtgaat
     6541 gggacggaat ctttggcagg aggagctgcc agggccggta gagaaggccc cagaagcacg
     6601 agaagaatca gccgcagggc ccaagaatgc ttgctgggcg gggaatgggg cactgcgagc
     6661 ctccaagact gtgtctcaag caaagaagcc tgtcagagaa tgtcggaaga cgcacccggc
     6721 ctcgaatctc ccgagtgtat tgacgccggc gggggtgcaa gagttagctg cgccaagcta
     6781 cgattctgtc tattttttga cctaagaggc gattcgaggg cgcattagaa gttgaaacgg
     6841 tagccgagaa tcatcttagc gatcaagtgg tccgtcgagt gcgtgatgcc gacgcccagg
     6901 ccgaaattga cctcccagtt cggagagagc ttcagatcga tcgtaggaaa gatctggtgt
     6961 tgttgctggc caataggaga aaactcggtg gccggaccga ctgacccgta gtactcgata
     7021 cccccggtga ttttcttggt gaagtcgtag ctgaatttca cgttgggcga gaacacgaag
     7081 ccctggtgga cactctcacc gtgcagcgac ttgtcaaagg ccgggttgaa cgaccagtac
     7141 caccgccccg actgtttgtc gatgatggga cgcatttccc aggtccacgt gtcggttgag
     7201 aatttacggc gctgctagcc gacctcgttc gaaaggctca agccaaccgg ccacttccat
     7261 ttcttgggga tccgcacgcg cgggcggata tggtcgccca cccaatccca tccctggcca
     7321 ttttgcgtgg aagtaaaaat atagaagcca gtttcaaacc agtccgtgaa gccgtgcgtg
     7381 atttcgatgg tttcgtggag ctgatggttg gttgggaaca ggccgttttc agaagtcctg
     7441 gagccctgaa acgcgaagtt gctgtgcagt tcgatcatgg tgtggtgtgg ctcaacctga
     7501 tcgtagccgt agacctggat ttcgtagttg tcctgggcgc gagaagacag cgccgatgcg
     7561 gcgttcgttg ccaaataaaa tgcgatcgaa attgcaaagc gcggtctcat ggccgattcc
     7621 tgaaagctcc caagagtagc ccagtctcat agctcggcaa acaggaatag atgcgcaaat
     7681 caggcgtgcg ggtgtgactt gtcgtagacg tccatcaact ggcggatgga ggcgtgggtg
     7741 tatttctgcg tggtggatag tgactggtgg ccgagaagct cctgaatggc gcggaggtcg
     7801 gcgccgtccg cgaggaggtg cgtcgcgaag gcgtgacgca tcgagtgcgg gtgcaggtcc
     7861 cagttgacgt tggcgatgcg aacgtatttt ttgacgatgc gcccgacgga gcgctgcgtg
     7921 aggcgccggc ccgaataatt gaggaagatc gcttccgtgt acggcccctg cccgccactc
     7981 gttcgatacg tttgctccaa gagctgttcg cgcagaggcc agtatttttc cagggcttcc
     8041 tgggccttac ccccgtaagg aacgatgcgt tccttgctgc ccttgccgcg gacgcggaga
     8101 atgcgttctt tttgctccat gtcggcaaga ttgaggccgg tgagctcgct gacgcgcagg
     8161 cctgcggcgt agagaagttc caggagcgcc cggtcgcggc gaaggagcaa gccttctccg
     8221 acttgcaccg aacgaccccg cttggctggc agattcatgg ccgatgccag cggaagcaac
     8281 aagccctttt tggaagtcac ccggccgccg agcgcccctt gtcccatccc gcccagctga
     8341 ttgaggaaac cgttcatctc ttccgcagag agcaccgaag ggatgcgctt cggcaacttc
     8401 ggcgtgggaa cgagacgcgc ggggttctct ttcagatgac tttcccggac gcagtacttg
     8461 aagaacgaac gcagggccgc aagcttccgc gcgatggagc ttttggcgag gccgtgatcg
     8521 tggagatgcg cgacgaattc acgaatcatc gagtgggtaa cgccagtgag tgcaggcggc
     8581 cgggccccgg gcggcgataa atacgcgaga aattgttcga ggtccctgcg gtagttcaga
     8641 atcgtatgcg gcgaggaatt cttcaccgac tgaaggtatt cgagatactt cgcgatcgct
     8701 tctttcattc gctgctacaa ctccgggggt gagacccaaa tacagtatag gcgcggacaa
     8761 atccgcggaa agcggaatcg cggcactgtt tctttagatg caggtgggtc aggacttggc
     8821 tgccacgggc accggagatt cctccggctg cgcttgcggc tgagcttcgg gaacaagcat
     8881 ctcccagtcg cagccctctt tgaggcaggc gcgcacggtg ccgatctttg tgcgcttctc
     8941 gacgatgaac ggtgcgccgc acttcgggca cggttcggcg atgggcatgt gcggcgtggt
     9001 gaaatcgcaa tccgggtaac gcgagcagcc gtagaacacg cgcgggcggc cacggccacc
     9061 tttgccggcg ctgccgcggc gcacgaattc gccttcgttg catttcggac atctaatgcc
     9121 catggtgatc gggcgcgtgt acttgcactt cggatatccg gagcagccga taaattcgcc
     9181 gaagcggccg tgctttttca caagattatt gctgcaaagt gtacacagtt cagcgagagg
     9241 ctcatcgggc tggtgcgcga tgcgcgtgcc ctggacgaga cggcgcgtgg tcttgcagtc
     9301 cggaaaaccg gtgcaggcga gaaaggtgcc gaagcgcccg cgcttgatgg ccatctcctt
     9361 gccgcaattg tcgcaatact cggtcttggg ttcgccaccg gtctcttcgg gaagttctgg
     9421 agacaggtcc tgaatgaaat cgcattcggg atagcgcgag cagccgagga agaagccgtg
     9481 gcggctgatg cgctcaagca actcgccctg gccgcacttt tcgcactttt tccccgtggg
     9541 aatgccagcc ttgtaggaaa gcatctcgtc gccggccttg tcgaggtcaa tgacgaattt
     9601 ttcccagaac tccttgacgg cctcgcgcca gggcagcttg ccctcttcga tttcgtcgag
     9661 ctcttcctcg agccgcgcgg taaacgtgac gtcgaagatg tcttcgaaac tctttaccag
     9721 cagcacgctg acgcgctcgc cgagcatggt gggcgagaaa cgtccctggt ccttggtgac
     9781 gtattcgcgc tcgacgatcg ttgaaatgat cgatgcgtaa gtcgagggac gcccgatgcc
     9841 gtcttcttcg agttccttga ccagtgtcgc gtcattgaac cgtggaggcg gctcggtgaa
     9901 gtgctggtcc gggcgaatct tgtccaagcg aagggtctgc ccttccgcaa cctggggaag
     9961 cgcgcggcct tcgccttcat catccttttc gtcgtcctcg cggtccgcga tcgacgcggg
    10021 catctgatag acgcggaggt agccgtcgaa tttctggacg gtgccggtag ctcggaaggt
    10081 gtaatcgccg gcaaaaatgt cgatggtcgt ctgatcgaag atcgccggaa gcatctgtga
    10141 cgccacaaag cgctgccaga tcagttggta gagcttgaac agatcctcgg cgagatactt
    10201 gcggacgtct tccggcgcgc gcatggcgtc ggtgggacgc accgcttcgt gggcttcctg
    10261 cgcgtccttc ttcgatttgt aatagttggg cttttccggg agatagttcg agccaaagcg
    10321 ctcggggatt agctcgcgaa cctgaatcag cgcatcgttc gaaacatgca cggagtcggt
    10381 acgcatgtag gtaatcagcg cgaccgagcc ttcgtcaccg agttccacgc cttcatagag
    10441 cttctgcgcg agtgacatcg tgcgcttggc ggtgtagcgc agccggttgt acgccgcctg
    10501 ctgcaatttg gaagttgtaa acggcggcgg aggattgcgc ttcttttctt tttgcgtgac
    10561 cccggcgacc tgccacttgg ccttactgac cgagccgaca atcttgtccg cgctctcctg
    10621 atttgagact gccatatctt cgcccttgtg cttgaagagc ttggcttcga agagcggcgg
    10681 ctgcccggca tccagcatcg cgtgaatcgt ccaatactcc tgcggaacga aggcgcggat
    10741 ttgcaattca cgctcgacga tcagccgcag cgccacggtc tggacgcgcc cggcggaaag
    10801 cccgcgacgg actttgtccc agagaatcgg agaaactttg tagcccacaa tgcgatcgag
    10861 cacgcggcgt gcctgctgcg catccactag attggtatcc acctgccggg gatgatcgaa
    10921 agccgcccga atagccttgg gcgttatttc attaaaggtg acccgaaaaa tctttttagg
    10981 gtcgatggcg acaacttctt ttttggaagc gctctcgtct tctttcttag cgggctcttc
    11041 aggtgttgca actttcttct taaatctaga aacgctgggt tcctgttcga tgaacttgga
    11101 aggcttggag agaaccatgg cgaggtgcgc cgaaatggct tccccctcgc gatccgggtc
    11161 gcccgccaga tagacggcat cggcagagat cgcagccttc ctcagatcgt ttatgacctt
    11221 gcccttgccc gcgataattt gcagcgtggg ttcaaaaatc ttttcgtcat cgatgctgat
    11281 cagtttcccg gctggctttt ccttctcctt tgcttttttg cttcctttct tggctttgct
    11341 ctttttcttt tttttgccgc cctcttcttc gccaggcaga cgaatgccaa tcgttttctt
    11401 gggcaggtcc atcacgtggc cgatggacgc ttttacgatg aagttccgcc ccagatattt
    11461 gttaatcgtt ttcgcctttg cgggcgattc gacaataacg agcgaacgag ccatgttgcc
    11521 tttgtaactc cgtaatgtat tgcagagagg gctgaacgcg cggcgtgtgt ccttctcgct
    11581 aacttaaacg cccggcgcgg gtcccaacga ggtctctctt ttgatgcggc gattgccttt
    11641 gtggatgcgt tttactaccg gatgccggcc tcaggccaac catgcaaacc tctatctgcc
    11701 tctacagcaa gactttgctg aactgtttgc ctggcaactg ccggatgatg cctttcatct
    11761 ccagatcaaa gagcgtagcc agaacttcgg aagagttcaa gccggagcgt tccacgatgt
    11821 catcaatggg caccggctcc tcagtgctga gcagttcata gagcttcttc tgcgagccat
    11881 tcagtgcggc agccgccaat agattccgct gctcggcctc cggttgctcg gcctgcagga
    11941 gggcggcacg gatgggcgtg ggcagctctt cgatcacgtc cgcggcgcag gtgacgagct
    12001 tggcgccctg cttgatgagt tggtttggcg cgaaactcac aggctgcgtg acgttccccg
    12061 gcacgccaaa cacttctctg ccgaactcca tggccaggcg cgcggtaatg agcgacccgc
    12121 tgtattgcgc tccttccacc accaccacgc cgagcggcat gccggcgacg atccggttgc
    12181 gaatgggaaa attctccggc gcggggtgcg tgcgcagcgg aaattcgctg atgattgcgc
    12241 cgcgctccag cactttttca tacagcttct tgttttcttt tgggtagcag acgtcgatgc
    12301 ccgtgccgag aactccgata gcgcggccgt tggcttccat ggctccctga tgcccgatgg
    12361 catcaatccc gcgggccagt ccgcttacga tgacgaggcc gcgcgcggca agttcccggc
    12421 cgagccgctg cgccatttgc gtgccgtaga gggttggccg gcgcgtgcca acgatgccga
    12481 tgctgggcag gttcaaaact tgcggatcac cgcgaacgta gagcagcacc ggcgggtcgt
    12541 agatttgtaa gagcgtctgc ggatattcgg gctcggtcca gttcaagagg gagcagcctg
    12601 caatcttctg aacgcccgcc aattccttct cggcgcgctt aaaggcttcc ttcttcacga
    12661 tggcttgggc gacttgagct gggagatggc aggcctcgag atcggaaaga gtggcccgga
    12721 agaccccatc cggcgagtca aaccgcctca gcacgcgggc ggacaaacgg gaagccaggc
    12781 cgggcgtgag ccccagcgca agccaagaga gggaagcatc cagctgcatc gcgggcgagc
    12841 ttacgcgtag agtaaccgac ttgtcaagtt cttatcgggg aatgatagct ggcgtgtgat
    12901 ttccatgcca agaaaagctt atgtcaggag cgatgttgcg ccgcgtcttc ttgcaggatg
    12961 gcatccagcg tcttgacgaa tagatccgca tcggattccg agaaaaccag cggcgggcgt
    13021 agcttgatga cgttgtgatg cggtccgtcg gtgccggcga gaacgcccca atcgcgaagg
    13081 cgattgacca cataggaggc ttgcaatgga gcaggctcgc gcgtttcacg gtcaagcaca
    13141 agatcgatcc ccaggaacag tcccgagccg cgcacgtcgc cgaccaatgc atggcgcgtc
    13201 tgcaaagact tcaagccctc gatgagatgc gcgccgacgc gcaaggcgtg ctgctttaaa
    13261 ttctcttctt cgagaacatc cagaaccgca agccccgctg cgcaagcgac aggattgccg
    13321 ccaaacgtgc tgaaaaactc catcccgttg ttaaacgaat tggcgatttc cggcgtggtg
    13381 ataaccgccg cgagcggaaa cgcattgccg atcggcttgc caagaacgac gatgtctgga
    13441 acaacgcctt gcgtttcgaa tccccagaaa tgcgtgccca gacgcccgaa tccgacctgc
    13501 acttcgtcgg cgatgcagac cgcgcctgct ccgcgcacgt gacggtaaac ctccgccaga
    13561 taatccggcg gaaaaacaat ttgcccgccg acgctgggca atgtttccgc aatgtacgcg
    13621 gcgactccac ggccattggc ttgcgtgcgc tccagtatct cggcgacgtg acgcgcgtat
    13681 tttgcgccgg cctgcttgtc gccccgccga tacgtgccgc ggtaatcatc cgcgagcgga
    13741 gcaacgtgca cccaggactt cttgccgcag cctccgggac cgtcgaattt ataggggctg
    13801 atgtcgatga gcgtattggt gtggccgtgg tacgcgtgtt ccagaacgat aatgtcttcc
    13861 cggcccgtat gcgcgcgcgc caggcgcaaa gccagctcat tggcctcgct gcccgagttc
    13921 acaaaaaaac aaacgcgcag cggagcgggc aaaagccgcg tcagccgctc ggcgtaacgg
    13981 ttcacgttgt cgtgcagata acgcgtgttg gtgttgagca gcgcgagctg cgcctgcgcc
    14041 gcctggacca cgcgagggtg gctgtgcccc acgagcggca cgttgttgta aacgtcgaga
    14101 tacgctcgcc ccgtgtcgtc atacaaatac tgcatccacc cgcgaacgat tttcagcggg
    14161 cgctgataag agatgctgag gtttttcccc aacaaggcgt ggcgtccggc aagcgtttcg
    14221 ctgagagcag gctctttggc cggaaaacga tcctccggaa ttcccaacag gagattcggg
    14281 tccggcgaga ggctcgtcca caccggacgc tgcgaagcgc gagcgacacc cggaaaatcg
    14341 tcgcccagtt cgagcagatc gagaatcacc tgaaaatgga gatgcggcgt ccagccgccg
    14401 ttttcttgtg ctgaaccaac acgcgcgaat tgctggccgc gtgcaatgcg ctggcccacc
    14461 ttgaggctgc caagcgcttc tttggagaga tgtccgtaga gcgtgaagaa ttcctcgccg
    14521 ccgctggttt catgccgcaa aattacgacg ggtccgtagt ccagcggagc ggaattattt
    14581 gccacgacct ggaccacgcc atccagcggc gcgctgagcg gagcgcccgg ctcaacaaaa
    14641 atgtccatgc caaggtgcac cgtgcgccgt tcgtccgtgg gattgctgct cgcgccgaac
    14701 agcagcgaag tgtacagcaa gcgtggttca tcgtaacggc cgatgccaac ggacgcgcca
    14761 gcgcgctgca tggcgtgaaa tactttttcg gtgagtgcgg gagtttccga tgcgcgcggg
    14821 tcggcgccaa gaaacgtact gccaacactc aaatcaaaaa cgacgctcgg agctgtccgc
    14881 aaatcaattt ccaaaataga agccgcggag cctgcgctcg cttccagcca cttcttgatc
    14941 ttttcacttt tcggaacggg cgaaagcccg caagccgctc gaaaagtgta gtgcgcgaaa
    15001 cgcgggtgga ttttttcgag cctctccaaa gcttcccacg ctggtgcttc gctaaccgtg
    15061 acgtacgggt catcaggctt aagggtcttg cggtgcgcgg aattggtgac gctgacggcc
    15121 agccgcgcgc ccatcaaagc gtaaagaacg gacagctcca gctcatcgag tggaaacgcg
    15181 ccgtgatatc ccgcgacgat ggcagttgcc gcctgcagtg ggtcttcctt cccaagaatg
    15241 ccgtaagcgg cggcaatcgc cgtctcagac gcggtgagcc cgtggtggat gtcgccaaaa
    15301 tcaatcacgc ctacgaccgt tcgcggctga ggccagggat cgccgaccag cacgttgtgg
    15361 tcgttcgcat cgccgtaaat tacgctgcgg cgcaagcgcg gcaagatggg aacggcttcc
    15421 gcttcataaa gcgcgagaaa ttttttcacc aacgcgcgac gcttcgaatc ggcgatatgc
    15481 ttgatgtagt ccctgatcca acccgcacgc gaagaatccc atttcagttc gcgatgcgcc
    15541 gcggcgtgcg tgaacgattg cagcgccgcg tccatctctc cgagaaaccg gccgagatcg
    15601 ccaagaagct ccggcgtgtg cgggcggact tcggcgagaa cttttccatt cacaaaagtg
    15661 agcagccaga cgaggcgcgt cgagccatcc gccgcggcaa tcgaactaaa taattcaccg
    15721 ctgcgattcg gtgtgacgcg aggcaaaggc aattgcggcg cgcgctgcgc cagatgcgtc
    15781 agggcccggc attgcagatc aataaaggaa ctcgcgcgcg ccgggtgcat tactttgagg
    15841 acgaacgcat gcccgtctgc gctcgtcaga tgaaagttgt cgtcgtattc gcccggcagc
    15901 gcccgcgcgg tggcctcaag cccgtaaaga tcgcgcgcca ggcgaaccgc ttccgcttca
    15961 gtcacgggtt gagctacgcg ggcttccagc agcatgcgcc aaatcttatc cgcaacagcc
    16021 ggagcgcgtc catcccggat ggctcaagaa ttccgccgag ccgcccttca acgcaaaatc
    16081 tttgcctctt atagtttctc tctcgagtct cggacggaaa ctggtccatt cactgtggaa
    16141 agccggatca ccgccggtgc gctgccatac tcgatgcggc ggtgctcgtc gtcccaagtc
    16201 ttgcgggcat tgtcgcaaat gctcgccttg caacttaccg gcgcattgtt cgttgattcc
    16261 accacgaagc tggactgata gccgctcggc accattagcg ttacgggacc gttcttcgca
    16321 tcggccgtga ggcccgtccc gctccaagtt ttcccttcga gcgccacgga gatcggcccg
    16381 ttctccgtat gaatacgcac gcttcccctg cttccgtcca gactgatcgg accattcacc
    16441 gcggtgatct ccgcgtcgcc ggaaaagttc ttcaagctga tcggaccgtt gtgggcccgg
    16501 gccgtgagtt tgccgtccac gtcgtacaga gaaatcggac cgttcattgt ttccagatcg
    16561 acggacgcgg atttcggcgt ccgaatgagc aggtagaccg tccaatcgtc ctcatccccg
    16621 gggcctttcg tcgagatctt gccgttttca atggacatgg tgatctggga caggattcgc
    16681 tccgcagcat cacccgaacc cgccgcggct ttgcatgccg tcaccgaata cgtctctttg
    16741 tcccagccca caacctgtac gccgccgttg ctatgcggct gaatccgcag gacaggagct
    16801 tcggacttgg tcagcgtgcg ctcttcggaa cgcatcacgg catcctggtc gtcaaagcgg
    16861 atgcgaagat cggagcaatc ggttgccggc tgtttatggc cgtcggaaat gctgactgaa
    16921 tggtggtgcc ggttcgctcg cgcgctcgta aacccgaggg cgagcagagc tgatatcgaa
    16981 atcagaaaaa acaagctgcg tcgagacatg gaggtcctcc ctgcagctat agacgacgga
    17041 agagtcgatt ggtcacgtgc gttccagccg aaatttcagc cccagtcatc ccaatcgtca
    17101 gccgtgccga taggtgggca gtccgggggt tcggccggtg gcgtgtcact gttttgaaat
    17161 aaccaggaag cttctgagcg cccgcggttt tctccgcgat cgtgggagtt tgcgccgttt
    17221 cgggctggcc ccaagtggca catggcgctg ccagcgcggc gctcatcaac aaaaggttgg
    17281 gccaaggctt cgacatatcc catccctcca aactcgccga atgcttttac aacagaaaca
    17341 gcaccatggc taacccttat cggtttagcg ggctttgtct aagcgattgg atgtaaaatg
    17401 aggccgaagt tggtctcggt ccgtgtgatt ataaggatat tcggaatgcg aagacccttc
    17461 ctccaggtac tcatgtgtgt tgccctgact gcggaggttt tgatctgcgg cttgtcggct
    17521 caagcgcagg taacgaaggg atgttcggaa ataaattacg ttggtgcgga ttccagcaat
    17581 ccctttactg ccgagtacgt tacgaccagc acgatgccca ctccggcggg agtgccgaaa
    17641 acgatggtgc tgagggaaaa cgtagccagg gacagtcagg ggcgaatccg gttcgagaag
    17701 cacggcgtgg ctcaacctcc cgatgaccgg aaaacagtga cgctagagac tccggatggc
    17761 aagccgttca cggtaacgcg ggaggagtat ggcactctca tcgacatttt tgactgtgcc
    17821 tcgggaacat tcgtccgtat ccagccggga atgcgaatcg tgaccgtgaa ggaaggcaag
    17881 agcgaggccc cggcaaggcg ggcaaaacat gcgtactcca ccccatactt ccccagccct
    17941 gggtccaaaa cactgccgaa cgtgattcag gaagaattgg gaacccggga tattcaaggt
    18001 attccagcgc gtggattgaa gaccacgacg ctcggaacag aaaaggatgc ggactggaac
    18061 ggcaaaccca tccgcgaaac ggagctttgg gtttcggatg acctggctgc gcaattgcta
    18121 aggattgata aggatctgcg gaccggaaac gaaggcagat ccgagctgct ggcaatcaag
    18181 agggaagagc ccgacccggc gttgttcgag atcccgaaag attgcgaagt gaatcccgcc
    18241 atgctgccga gccagaaggg tttaggtgta gttcgacctg cggggcagtg agcgaagcag
    18301 tcttcacttg gccgtatctt gtgctacctc gcaactgatc ctctaccatt gcttcttaat
    18361 ccttgactta attaacggag acggtacaat aggggcatgt caggtgggat ggtcccccta
    18421 ccggttcccc cggttaaagt gccctcccct aattctttcc ctccaccctc taagcccttt
    18481 gcttgccaca cttccgggat atcgctccaa tcctcgatat tatcggacgc aaattcaact
    18541 catttcgatt ccattctcgg tggcgctaac tcctttacta tcacatcgtt aaccgaccac
    18601 caccccttaa actctgtcgt atcatatcgt tacaaaaaca taggggggag ggggttagca
    18661 tcctactccc cagaattcga gcctcaatcc gctaaccctt gtatttgcca cacattcgca
    18721 aaatacaccc ataactcctt tctttgccac acatccaaaa acacagggtt taaagtcctt
    18781 tgtttgccac acatccgcga aaagtggggg tccagccttg aaccgttaac tagccatggt
    18841 tcacggatcg ccgctctccg ccgaccgaca agagtcggat ccacttcgcc gcgtctttcc
    18901 tatatttcaa gttcttccaa ccttccgaac tcgcagaagg attcctccca acagaagctt
    18961 gccgcccaaa aaaccggctt tgttatactg gaactgaaac gtgagccctc gaatcgtgac
    19021 gaaacttcgc atctctatcg ttcaatacct aaacaccgcg ccgctcgtct ggggcttcac
    19081 taatggcccg ctgcgcggca agtatgattt gtcgttcacc gtgccgtcgc tgtgcgccga
    19141 agagttgcgc aacggcaacg cagacgtcgc aattatcccg gccatcgaat atcagcgcat
    19201 cgatgatttg gtgattctgc cggacatggc cattgcctcg aagaagctgg tgcgaagcct
    19261 gctgattgtc gcgaagaaac ccatcgagca ggtgaagagt tttgtcctgg atcgcagttc
    19321 gcgcagcacg cagacgctga cgcgcatcct gtgcgcggaa aaatggagga tcgcaccgga
    19381 gttctttgag gcgccgccgg acttgggcgc catgttgcaa caagcagacg cggcgctcct
    19441 gattggcgat ccggctttgc gaattgcagt ggggatcgaa aaggaaagcc ggccgggcac
    19501 gccaggccaa acgttatgcc aggcggccgc tctcgggatt gccggtccgg aatttcttta
    19561 cgtctatgac gtcgtgggcg aatggcggag tttgacggga ttgcctgccg tcctggcggt
    19621 ctgggcggct aggcaggaca ttgccactcc tgatgtagcc gcggatttcc tcgcctccgg
    19681 ggatttcggt ttgtcgcgca tcccggaaat caccttcgac gccgcgcgcg aactggaatt
    19741 gccgcagccg gcgcttgagt cctatctgcg ccacaacatt gatttttccc tggataaaga
    19801 aaatcgccgc ggcctggaat tgtatttcgc acatgccgct aaactcggct tgattccgca
    19861 agtcaagccg atcaagtggg ctacgaaacc agaagccgtg aagttgtagg ggcgcagcat
    19921 gctgcgcccc agttagcgag atcattcatt cgagttcgca agagggaatg gttacaattg
    19981 atgtgggaaa gcgataagga accatcttgg gactaacgaa acaacaagct ctcgacatgc
    20041 tcgaatccga tgacctcgtg ggcctcggca tggctgcgca ccaagtgcgc ctgaaaaaaa
    20101 acgaccctcg cgtcgtcacc taccagattg accgcaacat caactacacg aatttctgca
    20161 cggaatactg ttccttctgc gcgttttacc ggccgctcgg cgccaaggac ggctacattc
    20221 tgtcgttcga ggagatctac aagaaaatcg acgaaatgct ggaactcggg ggcaccggaa
    20281 ttttgctcca gggcgggctg caccccgact tgcagatcgg ctattacgaa aatctattgc
    20341 gctccctgaa ggcacgctat ccgcaagtgc atttgcattg cttttcggcg cccgaaattc
    20401 tctgtatcgc ggaagtcagc gagatcagca tccgcgaaac gattcaacgg ctgatggacg
    20461 cggggctgca atccattccc ggcggcggcg cggaaatcct cgacgatgaa gtccgcgcga
    20521 agattgcgcg gttgaagtgt acgagcgacg actgggaaga aatgcaccgc gtggcccatt
    20581 cgctgggact gcgcaccacc gccaccatga tgttcggctg cggcgaagag ttgcgccatc
    20641 gcgtcaacca tctcgaaagg ctgcggcgga ttcaggaaga taccggcggg ttcacggcct
    20701 tcattccgtg gatgtttgcc actgaaaata ccgcgcttgg aaaaaaagtg caggaagcca
    20761 cagcggtcga ttacctgaaa acgctggccg tcagccgtct gtatctcgac aacgtcgacc
    20821 acatccagtc cagctggctc acgcccggaa tcaaagtctg ccagattggt ctgcaattcg
    20881 gcgccgacga cgtcggcagc atcctcatcg aagaaaacgt cgtcttcgcc gccggcgtga
    20941 agaatcgcac caacgaaagc gaactccgcc gcgtgatctc cgacgccggc ttcatccccg
    21001 cccagcgcga cacgctgtat cgcagttatg ttctgaaata atttctgccg ccgcgaagaa
    21061 ctctgcagca gcaaggcact tataaaggtt ttgggcgcag gtggatcgaa acatcgacga
    21121 ccatgccgtc gggcaactcc acatcttcca gaagatagag aacgccgccg cggaaacgcg
    21181 cctggacccg ccgcggttcg ggtttggagc ttggcggttc aggtttggag ccgatcggtt
    21241 caggtttctt tgtggggggc gcgccgggcg cctttccttc tgtttcatcc agcgcttcgt
    21301 tggccagggc gtcggcctcg cggttctgct cgcggtaaac gtgctcgatg cggaaggact
    21361 cgaaggcctt tgacattttt tgggcgcgct cgaaaagcgg acgcagatcc tcgctcttca
    21421 ctttgtattg cccgcgcatc tgcttgacca acagctcgga atcgctctcg atgcgaatcg
    21481 cgcgcacgcc gtgcgaatcc gcataatcca gcgcagcgat gagtccgtaa tattcggcaa
    21541 cgttgttggt catgcggccg atgtatttct tcagcttggc gacgatttcg ccgcgcccat
    21601 cgcggatgac cacgccgtaa gaagccggcc cgggattgcc gcgcgagccg ccgtcaatgt
    21661 ttgcgcgatg cgccgcagca gcgggcttgg gctccaaggg agcttcgccg aaaaggcctt
    21721 ttccacgggg agttggcggg cggcgcggca tcaggaattt gtcgaggccg ccgaggatgt
    21781 gctgctggaa ccaacggcgg gattcgcgat aggaattggt tccagggtgt acaggataaa
    21841 accgcaactc tcgcagcgga aaacttcttc gttggtttcc gtgcgcagtt cctgaagaat
    21901 atgcggcagg acgcgcatgc cgcacccgcg gcactgcccg tcgcgggctt cggccatggc
    21961 cgtgccgttg tggcgcttgg cgatgcgcgc gtaaagctcg cacaattctt ccgggaccgg
    22021 agcgatgatc cgttcgcgtt cggcggtggc ggtttcgagc tgtttctttt tctccgcccc
    22081 ttgcgactga atttcttttc gttcggcagc gacggcctgc tcggcttctt tcagtctgga
    22141 ctccgcgatc ttcacgcggc gttcgacttc ttccgcggcc atcatcactt cgagttggcg
    22201 gtcatccgct ttggccacct cggcctcggc gttgacgatt tcgtgctgca atgctttgaa
    22261 ggcttcgttg gttttcacgg cgccgctctg atcgcggtat ttgcgcgcgc ggtctttcca
    22321 ctgctgcccg tcgaactcga acttcttgcg ttccgcagtg ctctgggcgt gggcctcttt
    22381 cgccgaggcg acctcggcgc gggcgctgcc gagtttggtt tccgcttcgc ggatgcgctt
    22441 tggaaatcct tccagattcg cccgcaggag ggcgatctgg tggtcaacgc gttgcagctc
    22501 aatcaggtga ggaatcgccg gatgcatggg tagacaattc tagcacaggg acgggcgggc
    22561 tttgcgggaa ccgcggcggc ggatagaa
//
LOCUS       DASYVO010000009         3655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_239779, whole genome shotgun sequence.
ACCESSION   DASYVO010000009 DASYVO010000000
VERSION     DASYVO010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239779"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1478)
                     /locus_tag="VGR03_00450"
     CDS             complement(<1..1478)
                     /locus_tag="VGR03_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEV1992780.1"
                     /translation="MDRRDFLKTTSTVIAGATFAPGALAANPPLPGDSKAEGRMVLPI
                     NRNWRYNKAFVEGAHGREFDDSGFERVVIPHTNVRLPWHSFDDKTYEFVSIYRRRFKL
                     PPEAAGHHVFVDFEGVMTASTVWINGARLGEYKGGYTPFSFDLTPHINFGGENVLAVD
                     VDSTERPDIPPFGHQIDYLTFGGIYREVSLRIVPGTFIENIFAKPKDVLSGHPTLDVD
                     CFLQHLETSHETLTLEVELRDGERVLAKGTQKVPPSEASADPMAHTVHLDNLGAIKLW
                     DLTHPNLYSVHVRLMRGTQLVDQDKRTVGFREAQFTDHGFELNGKVIKLRGLDRHQTF
                     PFVGQAMPGRAQRSDARILRHKLHCNIVRTSHYPQSRHFLDACDEMGLLVLEEIPGWQ
                     HIGDEPWKQISIDNVSRMIRRDWNHPSIILWGTRINESRDDHDFYTRTNALAHRLDPT
                     RPTGGIRYFQESEFLEDVFTMNDFGFPLKPPNHPRYLNTEFVG"
     gene            complement(1516..2721)
                     /locus_tag="VGR03_00455"
     CDS             complement(1516..2721)
                     /locus_tag="VGR03_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992781.1"
                     /translation="MLPSDLKPEQFNGYPPEARKLVTGYVAALQRLPLSFLPSLLREA
                     VEYDFKFPAERRALEKELANLNSLSAEQTKEWLRGFAQIRLSSQLEHSDWVNAPGQFV
                     EQLSSYLWTTHQLDAFRIAALAYADRLRTVVPPEKPPVPRLGITIIGQGVSAHDEPLF
                     RKLRAHGAYFSGVKPENGLELLLNVVAARAKAHPVPYGHWYIDGGRAAAHDPALTCVS
                     YHALEPVRAALSAKIRSEIERPGMGPEALRTHLAQMRPADLGLGKAGDAVLDRFQVKM
                     LTEGSGTQVFSTTFAQWTAREALRRAQPLTLLVRFAPRRRQKPMNEMLSASHDPAELD
                     PLGSLIDADMGAYYNWLNLQRLPGAEQSSFLVWFEGHNEAIAIGPSLPRGTESNAAAS
                     LEQLLSWAS"
     gene            complement(2727..>3655)
                     /locus_tag="VGR03_00460"
     CDS             complement(2727..>3655)
                     /locus_tag="VGR03_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992782.1"
                     /translation="LMRELIPQSAFFTQVVNRGILGHYVATASLATGVYETINNFASL
                     PPEHPTVFEYFRKDLKRPASDTWVVAPSNGFNRIGESSHHSYGPGLGAGVILPKHLLA
                     AATSGGGSDYEHLLRDNYETPYNTPELGGSEFKLQQLGLMLKLSVDDFKAHARTLSSP
                     DELSVYIVRQLMRQLAPSLLWITMHDIDIAHAGAYSLYIDGIRRTDRLCADIWKTIQS
                     EPEYAGKTTLFILPDFGRDSDDDSGGNGFQHHRTGDALSRTTWMMALGAGVREGVVFD
                     RPLESTDLVPTLGSMLGFSASLAQGKPIPELL"
BASE COUNT          748 a          998 c         1160 g          749 t
ORIGIN      
        1 cccacgaatt ccgtgttcag gtagcgcggg tggttgggcg gcttcaaggg gaaaccaaaa
       61 tcattcatgg tgaaaacgtc ttcgagaaat tctgattcct ggaagtagcg gataccgccc
      121 gtgggccggg taggatcgag acggtgggcc agcgcgttgg tgcgcgtata gaaatcgtgg
      181 tcgtcgcgcg attcgtttat gcgcgtgccc cacaggatga tgctgggatg attccagtcg
      241 cggcggatca tgcggctgac gttatcgata gaaatctgct tccagggttc gtcgccgatg
      301 tgctgccagc cgggaatttc ttccagcaca agcaaaccca tttcgtcgca ggcatccagg
      361 aaatggcggg attgcggata gtgcgaagtg cgcacgatgt tgcaatggag tttgtggcgc
      421 aggatgcgcg catcgctgcg ctgggcgcgg ccgggcatcg cttggccgac gaacggaaac
      481 gtttgatgac ggtcaaggcc gcgcagcttg atgactttgc cattcagctc gaagccgtgg
      541 tcggtgaatt gtgcttcgcg aaagccaacc gtgcgtttat cctgatcgac cagttgagtt
      601 ccgcgcatga ggcgcacgtg gacggagtag agattcggat gcgtgagatc ccaaagtttg
      661 atcgcgccga ggttgtccaa gtgcacggtg tgcgccatag ggtccgctga agcctcggac
      721 ggcggaactt tctgcgtacc tttggctagg acacgttcgc cgtcccggag ttccacctcc
      781 agcgtgagcg tttcgtgcga cgtttcgaga tgctggagga agcaatccac atccagagtg
      841 ggatggccgc tgagcacgtc tttgggtttt gcaaatatat tttcaataaa cgtgccggga
      901 acgatgcgca gggaaacttc gcggtagatg ccaccaaaag tgaggtagtc gatctgatgg
      961 ccaaacggcg ggatatctgg ccgctcggtg gagtccacat caacagcgag aacattttct
     1021 ccgccaaagt tgatgtgcgg tgtgagatcg aaagaaaagg gcgtgtagcc gcccttgtat
     1081 tcgccgagac gcgcgccatt gatccagacg gtggatgcgg tcatgacacc ttcaaagtcg
     1141 acgaaaacgt ggtggccagc ggcttccggc ggcagtttga agcgccggcg ataaatggaa
     1201 acaaactcat aggtcttgtc gtcgaagctg tgccaaggca agcgaacatt cgtgtgagga
     1261 atgacgacgc gttcaaatcc ggagtcgtcg aactcgcgcc cgtgcgcgcc ttcgacaaaa
     1321 gctttgttgt atcgccaatt ccggttgatc gggagaacca tgcgcccctc ggccttggaa
     1381 tcgccgggga gcggcggatt ggccgcaagc gcgccgggtg caaatgttgc gcctgcaatc
     1441 accgtgctcg tcgttttcag aaagtcgcgt ctgtccatgt tttcctttca atttaataaa
     1501 aaatgtctcc tgccactagc ttgcccagct caacaactgc tccaagctgg cggcagcatt
     1561 ggattcagtc ccgcgcggca aggaaggtcc gatggcgatg gcttcgttgt gaccctcgaa
     1621 ccagaccaag aacgaagatt gctcggctcc cgggagccgc tgcaggttca gccagttgta
     1681 ataggccccc atgtccgcat cgatcaggga gccgagggga tcgagttcag ctgggtcgtg
     1741 gctcgcggac agcatctcgt tcatcggctt ctgccgccgt cgcggggcaa agcggaccag
     1801 caaggtgagc ggctgagctc ggcgcagcgc ttcgcgtgcc gtccattggg cgaaggtggt
     1861 gctgaaaacc tgggtacccg agccttcggt caacatcttg acctggaagc gatcgagcac
     1921 ggcgtcgccg gccttgccaa ggccgagatc cgcggggcgc atctgcgcca aatgcgtgcg
     1981 gagcgcctcc ggccccatac cgggtcgttc gatttccgac cgaatcttcg cggacaaagc
     2041 cgcgcgcact ggttccaggg cgtggtagga gacgcacgtt agcgcagggt cgtgagccgc
     2101 tgctcgccca ccatcgatgt accaatggcc gtagggaacg ggatgcgcct tggcgcgagc
     2161 ggctaccaca ttcagcagca gttcgaggcc gttctccggc ttgacgccgc tgaaataggc
     2221 gccatgggca cggagtttgc gaaagagggg ctcgtcatgc gcggagactc cctggccgat
     2281 gatggtaatt ccgaggcgcg gcaccggtgg tttttctggc ggtacaacgg ttcgcaaacg
     2341 atcggcgtag gcgagcgcgg cgattcgaaa cgcatccagt tggtgggtgg tccaaaggta
     2401 agacgagagt tgttccacaa actgaccggg cgcattgacc caatccgaat gttcgagctg
     2461 ggaggaaaga cggatttgcg cgaagccccg caaccactcc tttgtctgtt ccgcagagag
     2521 agaattcaga ttcgcaagtt ctttctccag cgcccggcgc tcggcgggga acttgaaatc
     2581 gtattcaacg gcctctcgca acaggctcgg caagaagctc agcggcagcc gttgcagcgc
     2641 tgcaacatag ccggtgacca gttttcttgc ttctggcgga tagccattga actgctccgg
     2701 tttgagatcg gatggaagca tcgaatctac aatagctctg gtatcggctt gccttgcgcg
     2761 agcgaagcag aaaaccccag catcgagccg agggttggca cgagatcggt cgactccaac
     2821 gggcggtcga aaaccactcc ctcgcgcacg ccggcgccca atgccatcat ccaggtggta
     2881 cgagagagcg catcgccggt gcgatgatgc tggaatccgt tgccgccgga gtcgtcgtcc
     2941 gagtcccggc caaagtccgg caggatgaac agagttgtct tgccagcgta ttccggttcg
     3001 ctctggatag tcttccagat gtcggcgcag aggcggtccg tgcgccggat accgtcaatg
     3061 tagagggagt aggctccggc gtgggcgata tcgatatcgt gcatggtgat ccacagaagg
     3121 ctgggcgcga gttggcgcat tagctgacgg acgatataca cggagagttc gtcggggctc
     3181 gacaacgtcc gcgcatgggc cttgaagtcg tcaaccgaga gcttcagcat caatcccaat
     3241 tgctgcaatt tgaactcgct gcccccgagc tccggggtgt tatacggcgt ctcatagtta
     3301 tcgcggagca aatgttcgta gtcactgccc ccgccggagg tggctgccgc caacaaatgc
     3361 ttcggcagaa tcactcctgc ccccaggccc ggaccgtagg aatggtggct gctttctcca
     3421 atgcgattga acccgttgct gggtgcaaca acccaggtgt cggaggccgg gcgcttcaag
     3481 tccttgcgga agtactcgaa caccgtcggg tgttccgggg gaagggaagc aaaattgttg
     3541 atcgtctcgt atacgccggt tgccaggctt gcggttgcca cgtagtggcc caggattccc
     3601 cgattcacca cttgcgtgaa aaaggccgac tgagggatga gttcgcgcat caggt
//
LOCUS       DASYVO010000010         3950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_127420, whole genome shotgun sequence.
ACCESSION   DASYVO010000010 DASYVO010000000
VERSION     DASYVO010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127420"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VGR03_00465"
     CDS             complement(<1..565)
                     /locus_tag="VGR03_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992783.1"
                     /translation="MPNRVGTQIFRTIDWAICKTAGITFDTIQYFNKRNPNPSVTPKW
                     SDKPLLKSWEKTKPTLGFPRQTDSLCPACVKEAREAIIAGKKDWRDLMSEKVGEIKAQ
                     IIERDGQVWMVKDCPLHGHYEDMMAVDSKWLSWIERQYPGRDIPAHNDEDLHKHGSST
                     VRYGRGSVLTVDLTNRCNMMCDPCFMDA"
     gene            complement(996..2129)
                     /locus_tag="VGR03_00470"
     CDS             complement(996..2129)
                     /locus_tag="VGR03_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992784.1"
                     /translation="MLADIGLRCYNASRAMADWKQITARIRRARTSKDPVGQLSNLFE
                     KTRDAMVAFELAKYFETSNQNAEAAKWYFAAAERFRRADWKTKAQEAAVRLGGVPGEI
                     PLETPTPRTDEFQLTPPAVTSPEADLPFEQNSAALESSVAVTSEVQQASEHSTSGPSP
                     SEAEGKRRRRGRRGGRNRRKGGAARETAGAPAPRVPATPVPRRGLPSAVFNEPAEPTT
                     PKVTHSRAIPSLPIESVGEPGGPSVKGRYGDPGLSSRISLLEMQFRRLLTCPPSRLDE
                     ADLAPVGQGVFVLTDADLTSYYYVEACQTLRIAIGNLMRGGSSRRGADSVKPQLAEHL
                     GIPEARVPKYLADHCVVRWLQLDEGASHFAHFVIAVLRPTLNE"
     gene            2232..2912
                     /locus_tag="VGR03_00475"
     CDS             2232..2912
                     /locus_tag="VGR03_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992785.1"
                     /translation="MSRLREAIAALISITLLLIPVWGSPSSSIGTVVYADRAHVGAAQ
                     ASVGATIFGGDRLTTEQAGGAQVRAGAARLLLSSASSATFLLDSGSPAATLTIGSATF
                     STASSKAFALRVASAVIRPNTDAPTIGQVTVLSPKELVVKSTRGSLSIAVEDDVRVIP
                     AGAAYRIVLDSTDAAAPQGPRGAGTKGYGGAPIKAAKSRFIWYAIGITALVTVWAFHE
                     ALESPDRP"
     gene            complement(3079..3456)
                     /locus_tag="VGR03_00480"
     CDS             complement(3079..3456)
                     /locus_tag="VGR03_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV1992786.1"
                     /translation="MRDIILTDRGPKPIGPYSQAIRTNGFLYVSGQVALDPRTGEMTG
                     ADIRQQTERTLENVKGILEAAGSNLHHVVKTTVFLKDMNDFAAMNEVYAKYFTSAPPA
                     RSTVQVARLPKDALVEIEVIAGL"
     gene            complement(3525..>3950)
                     /locus_tag="VGR03_00485"
     CDS             complement(3525..>3950)
                     /locus_tag="VGR03_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992787.1"
                     /translation="GLGDDTCVALVTDAHQHHHLFTTADSAVNLAGAGYSESQILEIA
                     KADQLEIISGDAVMLRLVGLSGPAVDIILHRRLRGQPTLSGAAIGRLKNTGLTEKQLL
                     ERINQGVTEAQAEKEAALREAVRNHSGTGFVRTHGRKPR"
BASE COUNT          757 a         1186 c         1123 g          884 t
ORIGIN      
        1 tggcgtccat gaagcaggga tcgcacatca tgttgcagcg attcgtaagg tcgacagtga
       61 gcaccgaccc acggccgtaa cgcaccgtgc tggagccatg cttgtgaaga tcttcgtcgt
      121 tgtgggcggg aatatcgcgg cctgggtact ggcgctcgat ccagctcagc cacttggaat
      181 cgacagccat catgtcttcg tagtggccgt ggagcgggca atccttcacc atccacacct
      241 ggccgtcgcg ctcgatgatt tgtgccttga tttcgccgac cttctcgctc atcaggtcgc
      301 gccagtcttt cttgcccgcg atgatggctt cgcgtgcttc tttcacgcac gcggggcaaa
      361 gcgaatcggt ctgccgcggg aagcccagcg tcggcttggt cttttcccac gacttcagca
      421 gtggcttgtc cgaccacttc ggcgtgacgg acggattcgg attgcgcttg ttgaaatact
      481 ggatcgtgtc gaaagtgatt ccagcagttt tacaaatcgc ccagtcgatc gtcctgaaaa
      541 tctgcgtgcc tactcgattc ggcataaacc cctaccctcc actttcgaaa atgcacaata
      601 cttggccttg gattatccgg agcccattca atcgtcggag ccgagcccaa cgactgcccc
      661 cgggctcaca catttgatgc atcaaaaccg taccacggtc tctaggtaca tccaataagt
      721 ctttgaacac ctatagtttg accgttaccc ccagcgggcg ccagcgagtt acgctgaacg
      781 gtcaaaatca gcggtcgaac ggtaacaagg gtcagcaaac gggcatttta ggggtctccg
      841 gagggggtta ctcctagtgc gaatgggctg caagtttgtc caaaaaccgc aggtatttcg
      901 gttgaagggg cgcagcactt gtggtgagaa cagccgaagc atgccgcacc cttacggcaa
      961 atggcctacc ccattgatct ctgaatggag caatcctact cgttgagggt gggacgaaga
     1021 actgcaatca caaagtgcgc gaagtgggaa gcgccttcat cgagctgcag ccaccggacg
     1081 acgcaatggt ctgccagata cttcggcact cgcgcttccg gaattcccag gtgttcggcg
     1141 agttgcggct tgaccgaatc ggccccgcgg cggctggagc cgccacgcat cagattgcca
     1201 atggcaatgc gcagcgtttg gcaggcttcc acgtagtagt agctcgtcag atctgcatca
     1261 gtgagaacga ataccccctg accgacgggc gcaagatcgg cctcatccag tctggacggg
     1321 gggcaggtca agagtctccg gaattgcatt tccagcaacg aaattcggga agacagcccg
     1381 ggatcgccgt agcggccttt tacgctcggc ccacccggct cacccacaga ctcgatcggc
     1441 aggctgggaa tcgctcggct gtgcgtcacc ttgggagtcg tcggctcggc cggttcattg
     1501 aagacagcgg aaggtaaacc acggcgtgga acaggcgtcg cagggacacg tggagccggt
     1561 gcccctgccg tttctctagc ggcaccgccc ttgcggcgat tccggccgcc acgacgtcca
     1621 cggcggcggc gtttcccttc cgcttcggat ggtgacggcc cgctagtgga atgctctgac
     1681 gcctgctgga cctcactggt caccgccacg ctggattcga gcgcggctga gttctgttcg
     1741 aacggtagat cagcctccgg cgatgtaact gcaggcggcg tcaactgaaa ctcatcggtt
     1801 cgcggggtgg gagtctccaa aggaatttcg cccggaacgc caccaagccg caccgcagct
     1861 tcctgcgcct tggtcttcca atcggcgcgg cggaatcgct ccgcggccgc gaaataccat
     1921 ttcgccgcct cggcattttg atttgaagtt tcgaaatatt tcgcgagttc gaatgccacc
     1981 atggcatccc gcgtcttttc gaagagattc gaaagttgcc ccacgggatc cttgctggtg
     2041 cgcgcccggc ggatgcgggc ggtaatctgt ttccagtcgg ccatagctcg gctggcattg
     2101 tagcagcgca agccgatgtc ggccagcatt gtccctcccc gctgagagag actctttctg
     2161 aagaacggtg tttaccgatt gactgcgcga accttaaaca gtattctttc cttggccctc
     2221 ttggaggcgt aatgtcgcgc ttgcgcgaag ctatcgctgc actgatatct atcactctcc
     2281 tgctcattcc agtctggggt tctccctcgt cttcgatcgg aaccgtcgtc tacgcggatc
     2341 gcgcgcatgt tggcgctgcc caggcatcag ttggcgcaac aatttttggc ggcgacagat
     2401 tgaccacgga acaagctggc ggcgctcaag tccgcgctgg tgcggcccgt ctgctccttt
     2461 ctagtgcgag cagcgcaact ttcttgctcg atagcggcag ccctgccgca acgctcacga
     2521 ttggctccgc gactttctcc actgccagtt cgaaagcctt cgccctgcgc gtcgcgtccg
     2581 ccgtgattcg cccgaacacc gacgcgccaa ccattggaca agtgacggtt ttgagcccga
     2641 aggaactggt cgttaagagc acacgcgggt cgctgagcat cgccgtcgag gatgacgtgc
     2701 gcgttattcc ggcaggcgcc gcctaccgca tcgttctcga ttcgacggat gctgcagctc
     2761 cgcaaggccc gcgcggcgcg ggcaccaagg gttatggcgg agcgccgatc aaagctgcga
     2821 agagcaggtt catctggtat gcgataggga tcaccgcttt ggtgaccgtg tgggcttttc
     2881 acgaggccct ggaaagcccc gaccgtccgt aattcacatc cgtcgttcga actttccatc
     2941 atcttatcga tcagagaagc catgtaggtt ttcgtgtcta cgaagggaca cggccgaggc
     3001 gcctgcatca gctctctctt ttcggttttc tatgttgact tttatttttc gtctgtgaac
     3061 tctttttctc acttcatctt acagccccgc gatgacctca atctcaacca gagcgtcttt
     3121 cggaagacgc gcaacttgca ccgtcgaacg cgcaggcgga gccgacgtga aatacttggc
     3181 gtagacctcg ttcatggccg caaaatcgtt catgtccttt aggaacaccg tggtcttcac
     3241 cacatgatgg agattcgaac ccgccgcttc cagaattcct ttcacgtttt cgagcgtccg
     3301 ctctgtctgc tggcggatgt cggcgcccgt catctcaccc gttctcggat ccagtgccac
     3361 ctgccccgag acgtacaaaa atccgttcgt tcgaatggcc tgcgaatacg gtccgatagg
     3421 ttttgggccc cgatcggtca aaataatgtc tctcacggtg tggtctcctt gtccgcacct
     3481 atagcatatg acgaattgcg ttgccaaata tgcgaggaga tagatcagcg aggcttgcgg
     3541 ccgtgagtgc gtacaaaacc ggttccggaa tgattgcgta cggcctcacg cagcgccgct
     3601 tccttttccg cttgcgcttc ggtcacgccc tgattgatgc gctcgagaag ctgtttttcg
     3661 gtgaggccgg tatttttcag gcggccgatt gccgcgccgc tgagtgtcgg ctggccccgc
     3721 agtctgcggt gcagaattat atccaccgcc ggaccggaaa gccccaccag ccggagcatg
     3781 acggcgtctc cgctgatgat ttcaagctga tcggctttcg caatctccag aatctgtgac
     3841 tcactgtacc cggcgccggc aagattgacg gcggaatccg cagtcgtgaa gaggtgatga
     3901 tgctggtgcg catcggtgac cagcgccacg caggtgtcgt cgccgagtcc
//
LOCUS       DASYVO010000011        12865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_197918, whole genome shotgun sequence.
ACCESSION   DASYVO010000011 DASYVO010000000
VERSION     DASYVO010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 12865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12865
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197918"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1950)
                     /locus_tag="VGR03_00490"
     CDS             complement(<1..1950)
                     /locus_tag="VGR03_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEV1992788.1"
                     /translation="MPEQAEVQFGREICGDLASAESREWLVTNGIGGYASGTVAGSQT
                     RRYHGLLVAALQPPVGRTQLVSAIDEIVRYAGTDFSLATHRWASGAVDPKGFLLLEGF
                     HLEGSTPVWTYALADALLEKRVWMRQGENTTYIQYTLVRGSSAFEMEWKALVNYRDFH
                     SLTHAGDWHMNIAPVEHGVKVLAFDGTTPFYLKSSAAAREPRHEWYLGCYFAEETERG
                     LSDREDRLFAALFRARLEAGSSVTLVVSTEAAASLDGETARAERVNHEVKLFQDWQAK
                     NEVLAEEAPSWLWQLIPAADQFIVKRSLPEEPEGRSIIAGYHWFGDWGRDTMIALPGL
                     TLATGRAGVARQILLAFSRYVDGGMLPNNFPDAGGKPEYNTVDAALWYFESIRQYFQA
                     TQDKATIEKLYTVLAGMIDVHLQGTRYNIHVDPADGLLYAGGPGVQLTWMDAKIGDWV
                     VTPRTGKPVEINALWINALETMAGFARLLAKPGEVYEKLAAKAKKSFQKFWNVERNCC
                     FDVIDSPGIGNDASLRPNQVFAVSLPVSPLTPEQQKAVVDVCARQLLTSHGLRSLAPG
                     EAGYTGHYGGSPRDRDAAYHQGTVWGWLLGPFALAHYRVYHDREAALRFLDPLGRQIY
                     ASGLGTLSEIFDGDAPFTPRGCIAQA"
     gene            complement(2035..2754)
                     /locus_tag="VGR03_00495"
     CDS             complement(2035..2754)
                     /locus_tag="VGR03_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1992789.1"
                     /translation="MSAKFEGKVVLVAGGTGGLGRAVTLAFLEEGAKVAVSYRAQEEF
                     GALKGAAGTNGSRLEGHTVDVTDEAAVHQLMEKIVGKHRRLDAMVNTVGGYAGGTKLW
                     ELETKVFEQMLALNLRSGYALSRAAVRAMLKEGHGAIVNVAAKAAVDHAAGAAAYVAS
                     KSAAVALLDSLAADLKGSGVRVNSILPSIIDTEANRKAMPKANFAKWPKPEDIARVIL
                     FLCSDDARVIHGAAIPVYGDS"
     gene            complement(2774..3250)
                     /locus_tag="VGR03_00500"
     CDS             complement(2774..3250)
                     /locus_tag="VGR03_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1992790.1"
                     /translation="MNSVPHLMIREATLQDEAELLRMMRHLAEHPPGKIQFDEPAARA
                     SFRRFLSLPAFGRVWLLCEGNLPVGYIVLTLGFSFEFHGHDAFIDELYIDAAHRRRGF
                     GRQAVTFVERKAREMGVNALHLEVDRGNDPAFELYRNTGYDDHDRFLMTKWLNREA"
     gene            complement(3243..5978)
                     /locus_tag="VGR03_00505"
     CDS             complement(3243..5978)
                     /locus_tag="VGR03_00505"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992791.1"
                     /translation="MPRIQTTEDRRLEEARTRKKHWKRWGPYLSERQWGTVREDYSPG
                     GTAWEFFPHDHARSRAYRWGEDGIGGISDRHQMMCFGLALWNGRDPILKERLFGLTGN
                     QGNHGEDVKEYYFYLDSTPTHSYMRMLYKYPQAEFPYETLLEESQRRGCGEPEFELLD
                     TGIFSGNRYFDLFVEYAKADVEDIFIRITAVNRGPEAAALHILPQLWFRNTWSWGKDL
                     RRPVARKATSVPGSNCAELQHWQYGKRWLLCAGQPELLFTENETNSVRLFGGRNRSPY
                     VKDAFHEYVVHGNKSSVNPGQMGTKIAAYYPLQLDPGQSVTLKLRLTDIEPLGGMDLN
                     SGKVGTITSPAHTERAEGVPGTNDFGAGFDGLFAMRQKEAEEFYASRIPAELSDDAKA
                     VMRQSFSGMLWSKQFYHYDVRTWLEGDPAGPQPPDDRWEGRNKDWAHLYNDDIISMPD
                     KWEYPWYAAWDLAFHCIPLALVDPDFAKEQLILLLREWYMHPNGQLPAYEWAFGDVNP
                     PVHAWAAWRVYKIERRVRGVADRGFLEKVFHKLLLNFTWWVNRKDPDGANIFQGGFLG
                     LDNIGVFDRSAPLPTGGHLEQSDGTSWMGMYCLNMLAIALELAKEDPAYEDVASKFFE
                     HFVYIARAMNDFGTGGRSLWDDADGFYYDVLKLPNGEEHFLKIRSMVGLIPLFAVETL
                     EPEIVDRLPGFKRRMQWFIDNHPDVPEHIEMTQRSARGVRRLLSLVSRKQLKRVLGRM
                     LDETEFFSPYGVRALSRYHKDHPYEVQVNGNTNRVDYEPAESSTGLFGGNSNWRGPIW
                     FPVNYLLVESLQKFHHYYGEDFKVECPTHSRTESDLWQVAAEISRRLTHIFLRDSNGR
                     RPVAGGSELFQNDPHWRDLILFYEYFHGDNGAGIGASHQTGWTGLVAKLIEQSGE"
     gene            complement(6119..6712)
                     /locus_tag="VGR03_00510"
     CDS             complement(6119..6712)
                     /locus_tag="VGR03_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992792.1"
                     /translation="MAKSSSDSSGGPSSQSLLFMMIAMTCGFCVLLGGGLLMASRVIS
                     ALGVRAGSDKTTVRTPLGEFRVEKAKEVGPGLPVYPAATLVLPGAAAAHLPLNDDQPQ
                     VIASTYHTNSSREYVVNWYLEHLSPEFARQDSGPKKLPEVFRDSRISDDDIAFVGERG
                     DQVRVVALALDDMGTKITLLRSYTKPPVQAPAQPPAQ"
     gene            complement(6959..10030)
                     /locus_tag="VGR03_00515"
     CDS             complement(6959..10030)
                     /locus_tag="VGR03_00515"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HEV1992793.1"
                     /translation="MRKQSGIALITTLLLLLLMSAMVVGFMLLVTEGQRLSGMNNEQS
                     RAFYGAESGMEKLTADLGSLFGTTYAPTGGQVDFLATQPPVLPSTTGVSYVDVLGAST
                     YAITYPKDARGNPQAQFAQITSGSSPFQGMTALETTYTMTVAARTKTGGEAKLVRTTQ
                     TVGIPLFQFGVYCEGDCSFFAGPNFNFGGRVHTNGNLFLNSGGPSGATPADTGKNQLW
                     LASPVTAAKDIFRDCLSNTNPESPTGQHPGSVEITKGGGAYQALGFGQGSLTGCLGTP
                     KNTSWPSISASFNGNLRSGVKPLNLTITLLGNGASQPIDLIRRPVQNENTINPGVLGE
                     RYFSEASLKILLSDTAADITGLPCVSVGAPLNLAHLAQPVPGWITNPDSLALYNLMLP
                     NAATTPMLPLAASGAAGGTNAVAGSYTQADGYWQATTTPIITGFIKIDAQTAYGAPCG
                     TWQDVTQEILSLGYAGRNIDPVPQSLDGATLNPQWTGTNAAMDLGKSPALPRLPGLNG
                     AGAPIQLSYQNGVAYPNGAALFTAISTTALPVASQGTCTDPHPNAVIRLERIRDNPST
                     VVFASGVKGTPAANLPKQSTVTQVCGVDPTTKAVLATLPTDYWPNVLFDTREGYNRQP
                     QPGGAFNNRVTLGGTMNYIELDVRNLARWFSGTIGATGAATRDPNVAPNNFVVYVSDR
                     RGNYVLPGTIAGTWPPLSPSGHETGEYGYGDFVNPATGAGCPNGALDTGEDLDGTAVL
                     YTYGEYSFPKFSPTTGLKDSTGSFTADTLYQGVVSSLTATVADPVCATGSSGISPWPR
                     TFLLNPNEARENPPALFRRALKLVNGSLITLPACPGGVSCGLTIATENPAYIQGDYNA
                     NSTVGGGFADPSVAASVIADAFTLLSNDWHDVNTFNSPFNVGGRLSNTAWYRLGVVAG
                     KGISFPIPAWDTTAVDGSQDFGTDGGVHNFMRYLERWGGTLNYTGSIISLYYNRQGIG
                     LYNSGTNNYSPPNRGYQFDSNFLNPLLLPPRTPMFRDINTTGFTQLLLPNQ"
     gene            complement(10066..11202)
                     /locus_tag="VGR03_00520"
     CDS             complement(10066..11202)
                     /locus_tag="VGR03_00520"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEV1992794.1"
                     /translation="MKKKSGFTLVELMIGMAVTMVALAAAVMIFRDSTRANTNVTQSS
                     DMSDNMRAGLNLIVQDLIQTGTGIPTGGITIPNTVNAAGCNIGKPVNRPPAVLNLTFQ
                     GPNSVNAGCNVILPAIEPGNSLGPTVTSPDGTSSPKTDIITILYADNTLALNQKPING
                     AACPAGAIAANGSTIKFDPACVSIGAAGIPVNPGDLLMLYNGNNPNGILQTVSSVSAP
                     TLNFNAGDAFNLNGRTASETSGTILTLQNLTAGVPNGTYPATSCTRVWMITYYLDATT
                     DPKHPMLMRAVNFNTPQPVAETLENLQFAYNFADGTTPAPVNQFAVPTVPAPSGDNEN
                     QIRSVNVYMGARSTTTAALTGKYVRTNLATQVALRSMAYFNTYK"
     gene            complement(11224..11787)
                     /locus_tag="VGR03_00525"
     CDS             complement(11224..11787)
                     /locus_tag="VGR03_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEV1992795.1"
                     /translation="MSRNRRRHNPQSGFTLLEAIIAIAILSFGILSLAAVYAQGIQVA
                     NMTQMDYIAEKKAEEAVETIFAARDSKLLAWTNIRNVTGSGGTNDGVFVVGPQPLLAA
                     GPDGLYGTSDDDTANPATVIMSAGADNILGTSDDVVMSLKNMTRTIAIADVSGESGLR
                     QLTITITYTAGSMTRQFTLVSFIAQFS"
     gene            complement(11799..12377)
                     /locus_tag="VGR03_00530"
     CDS             complement(11799..12377)
                     /locus_tag="VGR03_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEV1992796.1"
                     /translation="MRKCSNRSQQGFTLLEVLTAVAIIGIVLGMAMLNYGSILPNFKA
                     NAAMDQLLYQLRSARERAISHRREVQVQFIGTNQLQFTEIWLVGTAPVDKPVSFEGGA
                     QFIVFGSVPDIPAPFNFGNTAPVYFGGVSGGPPIMKFSTSGAFIDGGNTLVNGTVFMG
                     IPGRASTARAISILGATGRVREYHWDGTQWQE"
     gene            complement(12591..>12865)
                     /locus_tag="VGR03_00535"
     CDS             complement(12591..>12865)
                     /locus_tag="VGR03_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992797.1"
                     /translation="ASFTRDTVLGIGDLHGRVLVVDSTVGNQHTGHGNVLPSCLHGGP
                     GRAGGGEELAGLRALLLYHRRFVVQGPANCIIELSGSCVDTSLLYS"
BASE COUNT         2629 a         3605 c         3938 g         2693 t
ORIGIN      
        1 ggcctgggcg atgcagccgc gtggcgtgaa gggggcatcg ccatcgaaaa tttcgctgag
       61 cgtgccgagg ccgctcgcgt agatctggcg gccgagcggg tcgaggaaac gcagcgctgc
      121 ttcgcggtcg tggtagacgc gatagtgcgc gagtgcgaaa gggccgagca gccagcccca
      181 gaccgtgcct tgatgataag cggcgtcgcg gtcgcggggg ctgccgccgt aatggccggt
      241 atatccagct tcgcccggcg cgagactgcg caatccatgc gaggttaaga gctgccgcgc
      301 gcaaacatcg acaaccgcct tttgctgctc gggggtgagc gggcttaccg gcaaggacac
      361 ggcaaaaacc tgattcggcc gaagtgacgc atcatttccg atgcccggcg aatcgatgac
      421 gtcgaagcag caattgcgct ccacgttcca gaatttctga aaacttttct ttgctttggc
      481 cgccagtttt tcgtaaacct cgcctggttt tgcgagcaac cgggcaaatc ctgccatggt
      541 ttccagggcg ttgatccaca gcgcgttgat ctccacgggc tttcccgtgc gtggcgtcac
      601 gacccagtcg ccgattttgg cgtccatcca tgtgagttga acgccgggac cgccggcata
      661 gagaagcccg tcggccgggt ccacatggat gttgtagcgg gtgccttgta ggtgtacgtc
      721 gatcattcca gcgagaacgg tgtatagctt ttcgatagtg gccttatctt gcgtcgcttg
      781 gaaatattgc cggatggatt caaaatacca gagcgcggca tctacagtgt tgtattccgg
      841 cttgccccca gcgtcgggga aattgttggg gagcatcccg ccatcgacgt accgcgaaaa
      901 agcaagcagg atttgcctgg caacgccggc gcggccagtc gcaagcgtca gtcccggcag
      961 cgcaatcatg gtgtcgcggc cccagtcgcc aaaccagtga tagccggcga tgatgctgcg
     1021 gccctccggc tcttccggaa ggctgcgctt tacgatgaat tgatcggcag cggggatgag
     1081 ctgccaaagc cacgaaggcg cttcttccgc aaggacttcg ttcttcgcct gccaatcctg
     1141 gaacagcttc acttcatgat tgacgcgctc cgcccgtgcg gtttcaccat cgagcgaggc
     1201 tgctgcctcc gtgctcacca caagcgtcac gctggaaccg gcttcgagtc ttgcgcggaa
     1261 cagcgcggca aaaagacgat cttcacggtc gctcaggccc cgctcggttt cttcggcgaa
     1321 atagcaaccg aggtaccact cgtggcgcgg ctcgcgggct gccgcagaac tcttcagata
     1381 aaaaggagtt gtgccgtcga atgccagcac ctttacgccg tgctcgaccg gtgcgatatt
     1441 catgtgccag tcgcctgcgt gcgtcaagga atggaagtcg cgataattca cgagcgcttt
     1501 ccattccatt tcgaaagcac tgcttccccg cacaagcgtg tactgaatgt atgtggtgtt
     1561 ttcaccctgg cgcatccaga cgcgcttttc caggagcgca tcggccagcg cgtaggtcca
     1621 caccggcgtt gagccttcca ggtgaaaacc ctcgaggagt agaaagcctt tcggatcgac
     1681 ggcgccgctg gcccagcgat gagtggcgag cgagaaatcc gttccggcat agcggacgat
     1741 ttcgtcgatg gccgagacca gctgcgtgcg gcccacgggc ggctgcaaag cagcgaccag
     1801 cagtccgtgg tagcggcgag tctggctccc tgcaacggtg ccagaggcgt atccgccaat
     1861 tccattggtg acgagccact cgcgcgattc cgccgaggcg aggtcgccac aaatctcgcg
     1921 gccaaattga acttccgctt gttctggcac cggccgctcc ttggtcgctg aggattgcac
     1981 ccggcccggc aatccagggc cgcctaattc agattaattt cttgaaccaa agtttcagct
     2041 atccccatag accgggatgg cagcaccgtg aatgactctg gcatcgtcac tgcataagaa
     2101 caggatgaca cgcgcgatgt cctcgggctt cggccacttc gcaaaattcg ctttcggcat
     2161 ggccttgcgg ttggcctccg tatcgatgat gctgggaaga atagagttca cgcgaacgcc
     2221 gctgcctttc aagtccgcgg ctagcgaatc cagcagcgcc accgcagcag attttgaagc
     2281 cacgtaagcg gctgcgccag cagcgtgatc aaccgccgct tttgcggcga cgtttacgat
     2341 ggctccatgg ccttctttca acatcgctct taccgcagca cgcgatagcg cataccccga
     2401 gcgcagattc agagccagca tctgttcgaa aactttcgtc tccagttccc acaacttcgt
     2461 gccgcccgcg taaccaccca cggtgttgac catggcatcc aatcggcgat gcttccctac
     2521 gattttctcc atcaactggt gcaccgcagc ttcatccgtg acatcgaccg tgtgcccttc
     2581 cagtctcgat ccgtttgtcc ccgccgcgcc cttcaaagca ccgaattctt cctgcgcacg
     2641 ataggaaacg gcgaccttgg cgccttcctc aaggaatgcc agagtcacgg cacgtcccaa
     2701 tccaccagtg ccgcctgcca ccagaaccac tttgccctcg aatttcgcac tcatctgctc
     2761 acctctccgt tcattaagcc tcgcgattca gccatttggt catcaagaat cgatcgtgat
     2821 cgtcgtatcc cgtgttccga tagagttcga aggcggggtc gtttccccgg tccacttcga
     2881 gatggagggc attcacgccc atctcgcggg ctttcctttc gacaaaggta actgcctgac
     2941 ggccaaatcc gcgacggcgg tgggcggcgt cgatgtacaa ctcgtcgata aatgcgtcgt
     3001 ggccgtggaa ctcaaagctg aagcccagcg tcagcacaat gtagccgacc ggcaggtttc
     3061 cttcgcagag aagccagact cgcccaaatg caggaagcga caaaaaccgc cgaaaggaag
     3121 cgcgagccgc gggctcgtca aactgaatct ttcccggcgg gtgctcggca agatggcgca
     3181 tcatgcgaag cagctccgct tcgtcctgga gggtggcttc ccggatcatc agatggggca
     3241 cgctattcac cgctttgttc gattagtttt gcgacgagac cggtccagcc agtttgatgg
     3301 cttgcgccaa tcccggcgcc attgtcgccg tggaagtatt cgtagaaaag gatgaggtcg
     3361 cgccagtgcg ggtcgttctg aaagagctcg ctgccgcccg ctacgggccg acggccattg
     3421 gaatcgcgga gaaagatgtg agtgagccgg cgggaaattt cggccgctac ctgccagaga
     3481 tcggattcgg tgcgggaatg cgtggggcac tccactttga aatcttcgcc gtagtagtgg
     3541 tgaaatttct ggagggattc cacaagcaga tagttgaccg gaaaccagat gggcccgcgc
     3601 cagttcgaat tgccaccgaa aaggcccgtc gaggattcgg caggctcgta gtcgacgcgg
     3661 ttcgtatttc cgtttacttg gacttcgtaa ggatggtcct tgtggtagcg cgaaagagcg
     3721 cgcacgccat aaggcgagaa gaattccgtt tcgtccagca tgcggccgag cacgcgcttg
     3781 agctgcttgc ggctcactag cgagaggaga cggcgcacgc cgcgcgcgga acgctgcgtc
     3841 atttcgatgt gttcgggcac gtccggatga ttgtcgatga accattgcat gcggcgcttg
     3901 aagccgggga gacggtcgac gatttccggt tcgagagtct cgacggcgaa cagcggaatc
     3961 aatccaacca tcgaacggat tttcaggaag tgttcctcgc cgttcgggag ctttaagacg
     4021 tcatagtaaa agccatcagc gtcgtcccat agggagcggc cgccggtgcc gaaatcgttc
     4081 atggcgcggg cgatgtaaac gaagtgctcg aagaatttgc tggcgacgtc ttcgtaggca
     4141 ggatcttctt tcgccagctc gagagcgatc gccagcatgt tcaggcagta catgcccatc
     4201 caactggtgc cgtcggactg ttcgaggtgg cctccggtgg ggagaggcgc ggaacggtca
     4261 aagactccga tgttgtcgag gccgaggaat ccgccctgaa agatattcgc cccgtcagga
     4321 tccttgcggt tcacccacca ggtgaaattc aggagcaact tgtgaaaaac tttttccagg
     4381 aatccgcgat cggcgacgcc gcgcacgcgc cgctcgattt tatagacgcg ccaggcggcc
     4441 cacgcatgca caggcggatt cacatcgccg aatgcccatt cgtaggcagg caattgaccg
     4501 ttggggtgca tgtaccattc gcgcaataga agaatgagct gttccttggc aaaatcggga
     4561 tcgacgagcg cgagcggaat acagtggaac gccaagtccc atgcggcgta ccacggatac
     4621 tcccatttat ccggcatgga aataatgtcg tcgttgtaga gatgcgccca atccttgttg
     4681 cggccctccc agcggtcgtc cggcggctgg gggccggcgg gatccccctc gagccaggtg
     4741 cgcacgtcgt agtgataaaa ctgcttggac cagagcatcc ctgaaaacga ttgccgcatg
     4801 acggctttcg catcgtcgga aagttccgcg ggaatgcgcg aggcgtaaaa ttcttcggct
     4861 tctttttggc gcatcgcgaa caggccgtcg aatccagcgc caaagtcgtt ggtgccggga
     4921 acgccctcgg cacgctcggt gtgcgcggga gaggtgatgg tgccgacttt tccggagttg
     4981 agatccattc cgccaagggg ctcgatatcg gtgagtcgca gcttgagcgt gacggactga
     5041 ccggggtcaa gctgtaacgg gtaatacgcg gctattttcg ttcccatctg cccaggattg
     5101 acggaactct tgttgccgtg aaccacatat tcgtggaaag cgtctttcac gtagggggag
     5161 cgatttcttc cgccgaagag acgcacgctg ttggtctcgt tctccgtgaa aagcaactca
     5221 ggctgaccgg cgcacagcag ccagcgcttg ccgtactgcc aatgctgcaa ttcagcgcag
     5281 ttgctcccag gtaccgaagt ggcttttcgg gcgaccggac ggcgaaggtc cttgccccag
     5341 gaccacgtgt tgcggaacca gagttgcggc aggatgtgca acgcggcggc ttccggcccg
     5401 cgattcacgg cagtgatcct gatgaaaatg tcttcgacgt ctgctttggc atactcgacg
     5461 aaaaggtcga aataacgatt cccggaaaaa atgccggtat cgagcaattc gaattccggc
     5521 tcgccacagc cgcggcgctg gctctcttca agcaaggttt catacggaaa ttcggcttgc
     5581 ggatatttgt agagcatgcg catgtaactg tgggtgggcg ttgaatcgag gtagaagtaa
     5641 tactccttca cgtcctcgcc atgattgccc tgatttccgg tgaggccgaa gaggcgttct
     5701 ttcagtatgg ggtcgcgccc attccatagc gccagcccga agcacatcat ctgatgacgg
     5761 tcgctgatgc ctccgatacc gtcttctccc cagcgatagg cgcgtgaacg cgcgtggtcg
     5821 tgcgggaaaa actcccaggc cgtcccgccc gggctgtaat cctcgcggac cgttccccac
     5881 tgccgctcgg agaggtacgg cccccagcgc ttccaatgct tcttgcgggt gcgcgcttct
     5941 tcgagccggc ggtcttcggt ggtctggatt cgtggcatgg ttgaatgatc ctctgccgaa
     6001 agtttcaggt tatggtcgca cttttccccg ataacagcag cgttgacgtc gaagcgcgtg
     6061 cctgttccga cacttccggc aagtgctcaa gctgctcgcg taatggcgag cgacatttct
     6121 actgagctgg tggttgggca ggagcctgga cggggggttt ggtgtaagag cgcaggagag
     6181 tgatcttggt gcccatgtcg tcgagggcga gggcaacaac gcgaacttgg tcgccacgtt
     6241 cgccgacgaa cgcgatgtcg tcatcggaaa tgcgcgagtc gcggaaaact tcgggtaact
     6301 tcttggggcc gctgtcctgg cgcgcgaatt ccgggctcaa gtgctcgaga taccagttga
     6361 caacgtattc gcgggacgag ttcgtgtgat acgtgctggc aatgacctgg ggctgatcgt
     6421 cattgagcgg gaggtgcgcg gccgccgcgc cgggaagcac taaagtcgct gccggataaa
     6481 caggcaggcc ggggcccact tccttggcct tttcaacgcg gaattcgccg agaggagtgc
     6541 gcacggtcgt cttgtcactt ccagcgcgga cgccgagggc actgatcacg cgcgaggcca
     6601 tcagcaagcc gccgccaaga agaacgcaga agccgcacgt catggcaatc atcatgaaga
     6661 gaagcgactg ggaggaggga cctccgctag agtctgaaga agatttcgcc ataacgcgct
     6721 ttctcccctt agtacacgac cgcgccgagc cctactcgtt tcatcgaagc gggcaccgga
     6781 gatgccgcac cgacgaacag tgacgaggaa cccagcaata cgggtacaga tctaatcccg
     6841 ctgtatcgaa actcgattcg ccttagctaa cccgctccac gctactcttc ttcatcccgc
     6901 cgaaagaacg cggccccggg gacacggggc cgcgcgctgt gcagcagccg gccgcagatt
     6961 actggttcgg cagaaggagc tgcgtaaatc ctgtcgtgtt gatgtcgcga aacatcggcg
     7021 tccgcggcgg caggagcaaa ggattcagga agttggaatc gaactggtag ccgcggttcg
     7081 gcggactgta gttgttggtc ccggaattgt agaggccaat cccctggcga ttgtagtaca
     7141 ggctgatgat ggaaccggtg tagttcaagg ttccacccca ccgttccaag taccgcataa
     7201 agttgtgcac gcctccgtcg gttccgaagt cctggctgcc gtcgacagcg gtggtgtccc
     7261 aggcgggaat ggggaacgaa attccctttc cagcaacgac gcccagccgg taccaagccg
     7321 tgttcgacaa gcggccaccg acgttgaacg gagaattgaa ggtattgacg tcgtgccagt
     7381 cgttggagag gagcgtgaag gcatccgcga tcacggaagc tgccacgctg ggatcagcga
     7441 aaccgccgcc taccgtcgaa ttggcgttgt agtcgccctg gatgtacgcc gggttttccg
     7501 tcgctatggt cagaccgcaa gagacaccgc ccgggcaagc cggcaaggtg ataaggctcc
     7561 cattgacaag cttgagagct cggcggaaga gtgcgggtgg gttttcccgc gcttcatttg
     7621 gattcagcaa gaaagttctg ggccaggggc tgatgccgct tgatccggtg gcgcaaaccg
     7681 ggtctgccac ggtcgcggtg agcgagctca caacgccctg atagagcgtg tccgcggtaa
     7741 agctccccgt actgtccttc aaccctgtgg taggagagaa cttaggaaat gagtactcgc
     7801 cgtaggtgta gagcactgcg gtgccgtcca ggtcttcacc ggtatcgagc gcgccgttcg
     7861 ggcaacccgc accggtcgct gggttgacga agtcgccgta cccgtattct ccggtttcgt
     7921 ggccggaagg cgagagcggt ggccaagtgc ctgcgatggt ccccggaaga acgtagttcc
     7981 cgcgccggtc agatacgtac accacgaaat tgttcggggc tacgttggga tctctggtgg
     8041 cggcaccggt tgcaccaatg gttccggaga accacctcgc cagattcctg acgtccagct
     8101 cgatgtaatt catcgtgccg ccaagcgtga ctctgttgtt gaaagctccg cccggctgag
     8161 gctggcgatt gtagccttca cgagtgtcga ataggacgtt tggccaatag tcggttggca
     8221 gggtggcgag aacagccttg gtcgtgggat ccacgccgca aacctgggta accgtggatt
     8281 gcttgggcaa attcgctgcc ggtgtcccct tgactccgga ggcgaagaca actgtagaag
     8341 gattgtcgcg aatacgttcc agacggatga cggcgttggg atgcgggtcc gtgcaagtgc
     8401 cctggctagc caccgggagc gcagtggtcg aaatcgccgt gaacagcgcg gcgccgttgg
     8461 gataagccac tccattctga taggacaact ggataggagc gccggcgcca ttcaggccgg
     8521 ggagtctggg aagggctgga gatttcccaa gatccatggc cgcgttggtg cccgtccact
     8581 gcggattaag tgtggctcca tccagcgact gaggaaccgg atcgatgttc cttccggcgt
     8641 agccgaggct caggatctcc tgggttacgt cttgccaggt accgcagggg gcgccatagg
     8701 cagtctgagc atcaatcttg ataaagccgg tgatgatagg tgtggtggta gcctgccaat
     8761 agccatccgc ctgggtgtaa cttcccgcca cggcattggt cccgcctgca gcgccggagg
     8821 ccgccagcgg caacatcggc gtcgtggcgg cgttaggtag catcaggtta tatagtgcaa
     8881 gggaatccgg attggtgatc cagcccggga caggctgagc gaggtgcgcc aagttgaggg
     8941 gagcgccgac gctgacgcaa ggcaggccgg taatgtcggc agcggtatcg gagagcagaa
     9001 tcttcaagct cgcctcggaa aagtagcgct cgccgagcac gcccgggttt atggtgtttt
     9061 cgttctgcac cggccggcga atcaggtcga tgggctgcga tgcgccattg ccaaggaggg
     9121 tgatggtcaa gttcagcggt ttgacgccgc tgcggagatt gccgttaaaa gaagcggaga
     9181 tgctgggcca gctagtgttt tttggagtac caaggcaacc tgtcagactt ccttgcccaa
     9241 aacccagagc ttggtacgcg ccaccgccct tggtaatctc gacgctgccg ggatgctgtc
     9301 ccgtggggga ctcaggattg gtattcgaca agcagtcgcg gaagatatcc ttggccgccg
     9361 tgacgggact agccagccag agttggttct tgccggtatc agcaggggtg gcgcctgatg
     9421 gtccgccgct gttgaggaac agattgccgt tggtatgcac gcggccgccg aaattgaagt
     9481 tggggccagc gaaaaaactg caatctcctt cgcagtagac gccaaactgg aagagtggga
     9541 tgccgacggt ctgcgtagtc cgcaccagtt tagcttcgcc acctgttttt gtgcgagccg
     9601 caacggtcat cgtataagtt gtctccagcg cagtcatgcc ctgaaagggg ctggagcccg
     9661 aagtgatctg ggcgaactga gcctgcgggt tacctctagc gtcctttgga taggtaatcg
     9721 cataggtgct ggcgcccaat acgtccacat aggaaacccc ggtggttgaa ggaagaaccg
     9781 ggggctgcgt cgccaggaaa tcaacttgtc caccggtcgg ggcataggtt gtgccgaaga
     9841 gcgagccaag atccgcggtg agcttctcca tgcccgattc cgcgccatag aaagctctgc
     9901 tctgctcgtt gttcattccg gagagtcttt gcccttccgt caccaagagc atgaagccca
     9961 ccaccatggc ggacatcagc aggagcagaa gcagggtggt gatgagtgca atgcctgatt
    10021 gttttcgcat ggttgttttc accgatctca tcgtgaagct cttcattact tataggtatt
    10081 gaaataagcc atgctgcgga gagcgacttg cgtggccaga ttggtgcgga catatttgcc
    10141 agtgagtgcc gcggtggtgg tggaacgcgc gcccatgtag acgtttacgg agcgaatctg
    10201 gttctcgttg tcgcccgagg gagctggaac cgtcggaact gcgaattggt tcaccggtgc
    10261 gggcgtggtg ccatccgcaa aattgtaagc aaactggaga ttttcgaggg tctctgccac
    10321 cggttgtggg gtgttgaagt tcaccgcgcg catgagcatg ggatgcttgg gatcggtagt
    10381 ggcatccaga tagtaggtga tcatccagac gcgggtacag gatgtggcag gataggtgcc
    10441 gttgggcact cccgctgtaa ggttctgaag ggtcaggatc gttccggaag tttccgacgc
    10501 agttcgcccg ttgaggttaa acgcatcgcc agcgttaaaa ttcaaggttg gggcactgac
    10561 cgagctcacg gtctgcagga tgccattggg attgttacca ttgtagagca tgagcaggtc
    10621 gccgggatta acgggaatgc cagccgcacc aatggacacg caggctggat caaacttgat
    10681 tgtcgatcca ttcgccgcga ttgctcccgc gggacacgcc gcgccattga tgggcttctg
    10741 attcagcgcc agagtattgt cggcatacag gatggtgata atgtcggttt tcggactgga
    10801 agtgccatcg gggctggtga ccgtaggacc caaactgttg ccgggctcaa tggccgggag
    10861 gattacgttg catcccgcat tgacggagtt ggggccctgg aaggtcaggt tcaatacggc
    10921 gggaggccgg ttgactggtt tgccgatgtt gcagccagcc gcgttaacgg tgtttgggat
    10981 agtaataccg ccggtgggga taccggttcc cgtttgaatc agatcctgaa cgatcagatt
    11041 gagtccagcg cgcatgttgt cgctcatgtc cgaggactga gtgacgttgg tattcgccct
    11101 ggtggaatca cgaaaaatca tgacggctgc cgcgagcgca accatggtga cggccattcc
    11161 gatcatcaat tccacaagcg tgaaacctga ctttttcttc atgagttgct cctcaatcca
    11221 cgatcacgag aattgcgcta tgaaactaac cagtgtgaac tgccgcgtca tggaacccgc
    11281 agtgtaggtt atagtgatgg tgagctgacg tagtcccgat tcgccgctga cgtccgcgat
    11341 ggcgatcgtg cgcgtcatat tcttcaacga catcaccacg tcatccgagg ttcccagaat
    11401 gttgtctgcg ccggcactca tgatcaccgt ggccgggttg gctgtgtcgt catcggacgt
    11461 gccgtacagc ccatcgggcc cggcagcgag aagcggttgc ggtccgacga cgaaaacgcc
    11521 gtcgttcgtt ccgcccgatc cggtaacgtt gcgaatgttt gtccaggcga gcagtttgga
    11581 gtcgcgtgcc gcgaaaatcg tctccacggc ctcttcagcc ttcttctcgg cgatgtaatc
    11641 catttgcgtc atgttcgcta cctggatgcc ctgcgcgtag accgcagcca aggacaagat
    11701 gccgaaggac agaatcgcga tggcaatgat cgcttcgagc agcgtgaagc ccgactgtgg
    11761 attgtggcgc cgtctatttc gcgacatgag catcctccct attcctgcca ctgggtaccg
    11821 tcccagtggt attcacgcac gcgaccggtg gcccccagga tggagatggc tcgggccgtc
    11881 gaggccctgc ccggaatgcc catgaagaca gtgccgttta ccagcgtgtt tcctccatcg
    11941 atgaaagcac cgctcgtcga gaacttcatg atcggtggac cgccggagac tcccccaaag
    12001 tacacggggg cggtatttcc aaaattaaaa ggtgccggaa tatccggaac gctaccgaaa
    12061 actatgaatt gagctccacc ttcgaaggag actggcttgt ctacaggtgc cgtaccgact
    12121 agccagattt ctgtgaattg gagttgattc gtgccgataa attgcacctg gacctcgcgg
    12181 cgatgggaga tggccctttc gcgcgccgag cgcaactgat agagcaactg gtccattgcc
    12241 gcgttggcct tgaaattcgg caggatgctg ccgtagttga gcatcgccat ccccagaacg
    12301 atgccgataa tggcaacggc ggtgaggacc tccagtaggg tgaaaccttg ttgcgatcgg
    12361 ttcgagcact ttctcattcg tgtactccaa gaagcttccg ggtcaaagac ggccccgggc
    12421 agtctaagaa cagcacgttc gcacccaact gattattgtg gttaagtact ttagatttag
    12481 tgcgttagga aaaaggctgg tggggtatgt gtgaagcgtt gttgtaacaa aaagttacaa
    12541 accacgtcgc aatcaactgg gcttttggat tcgccccggt gagaggaggg ctaggaatac
    12601 aagaggctag tgtcaacgca cgatccggac aattcgatga tgcaattggc cggtccttgc
    12661 accacgaaac ggcgatgata gagcagcaac gctcgcaagc cggcaagctc ctcgccgcct
    12721 ccggcccttc cgggaccgcc gtgcagacaa ctgggcagca cattgccgtg ccccgtgtgc
    12781 tgattgccaa ccgttgaatc cacaactaac actcttccgt gaaggtcgcc gattccaagg
    12841 accgtgtcgc gggtgaaact ggcat
//
LOCUS       DASYVO010000012        11386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_225782, whole genome shotgun sequence.
ACCESSION   DASYVO010000012 DASYVO010000000
VERSION     DASYVO010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 11386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11386
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225782"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VGR03_00540"
     CDS             <1..314
                     /locus_tag="VGR03_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992798.1"
                     /translation="QDGRIPWALTAAGIYNRIRGFAPWPGAFTTFRARLCQLWGTPSP
                     GTTPAPVGRIFTVDGDLFVACGSGTLLRLESVKLEGRKRVGAQEFASGVRLKDGESFG
                     P"
     gene            411..2201
                     /locus_tag="VGR03_00545"
     CDS             411..2201
                     /locus_tag="VGR03_00545"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEV1992799.1"
                     /translation="MNDHRGRNRPLGRWTAGSRILLSFLLAFQCSSAQLTRAADSSAP
                     TKLAPDRVLETMKAELARAKKELAKADPAPYFLSYTVHDQSIVVLAAAFGSLLTNASA
                     QRREADVVMRVGSPLLDNTHGQSRSSGVTSGALPLGDDTDAIARVLWELTDREYRRAA
                     PAYLNVKTNTAVRAEEEDKSPDFSKETSQTSAAKPAAAPSLDRAAWEGEIRRLSAAFR
                     KYPEVYSAFVVLQLQSSTSRMFSSEGTEIVIPSSTARLVIDGQTRAEDGMELLRIETF
                     QAPSPAELPAESELISKIEKIALDLQALRKAPVAEPYDGPAVLSGRAAAVFFHEVLGH
                     RLEGHRQRDEEEGQTFTKKVGLEVLPKFLSVADDPTVRQIHGTKLAGSYDFDDEGVAS
                     RRVQVIQDGVLKSFLMSRMPIKGFENSNGHGRNQPGLMPTGRQGNLIVTSTQTASDAD
                     LRKRLLEEVKKQGKPYGLYFEDVQGGFTLTTRSLPQAFQVLPVMVYRVYPDGRPDELV
                     RGVDIVGTPLAALTRILLTGDKEQIFNGVCGAESGSVPVSAVAPAMLFSEMEVQKRAH
                     SHTRPPILPPPGFETTPATPDAKPTPGVKP"
     gene            2198..3907
                     /locus_tag="VGR03_00550"
     CDS             2198..3907
                     /locus_tag="VGR03_00550"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEV1992800.1"
                     /translation="MRAARFLLAVLSLAPLALLTSTSLCASTDDAAGKSDPAAKARAA
                     AAGDPLLEALLTELERSKSQLKMDQVQPPYYIEYRVHEVNLFAVEAAFGALREEQNTR
                     ARVLRVVVRVGNYKQDSFFGQGMGETTILPLDNDSIAIRHQIWLATDEAYKAASEALT
                     EKQAFMKQFAADSNPVDDFARATPINSIGSLAQLRIDSAAWRKTLEESTNLYRQYPAL
                     QSLSASARFAVVNEYFVNSEGTVTRSGHASYTVTLDGATQAADGMRLNRSPYWTVARP
                     EELPGRDALLGETRKMLDALMALREAPIAEEEYRGPVLFSADAADDMFAGLIGANILG
                     RKPQPGRPNRTTGAFATSFKSRVLPSFLTVVDDPTVKQFHGESLVGAYDVDSEGVLGQ
                     AIVAIDNGNLTNYLVGRQPIRDYPASNGHGRAAPGVFPQPSLGNLFVRTSEPKPVAAL
                     RQRMLEMVRAQGKPYAYRVETLGPGNSPRLLYRVHEKDGHEELVRGAVFNELDVRTLR
                     NNLAAAGDDPLVSNRAGGVLTTIVSPSILLDELEVKRADTSKDKLPEYPPPPLAQTNA
                     KKP"
     gene            3904..4668
                     /locus_tag="VGR03_00555"
     CDS             3904..4668
                     /locus_tag="VGR03_00555"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEV1992801.1"
                     /translation="MTTPDDPSSLPETPEITLRERLERVSRMALLVFILASAAFLSAI
                     TTMRIAIHGREVTMPNLVGKGVAEANGHVTSLGLVLRVADRVYSDLPMNVVVRQTPPP
                     GMLMKVSQEAHVVLSLGQRNLTIPPLEGKSLRASRIELLRTGLQIGEVSALALPDAAP
                     DTVVIQNPRPGSTAATPRVDVLVVQPPRENAFVMPHLVGLSEAEAQRRLDQSSVHHKE
                     NYMAAPQWPRGVVIDQTPVAGARLAASSTVELTVAN"
     gene            4824..5516
                     /gene="rpe"
                     /locus_tag="VGR03_00560"
     CDS             4824..5516
                     /gene="rpe"
                     /locus_tag="VGR03_00560"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV1992802.1"
                     /translation="MKSATPSVEIAPSILAADFSSLGEAVRAVERAGVKLIHVDVMDG
                     HFVPNISIGAPVVASLRKATQLILDVHLMIERPEEYIEAFAEAGANWITVHQEATVHL
                     NRAVHQIHEVGCRAGVAINPATPVSALADILPDVDLVLVMSVNPGFGGQHFLPASTKK
                     IAELRELRSRYTHNFRIEVDGGIAADTVQGVVRAGAEILVAGTSVFHKEDPAAAVHEL
                     QRIAAEAVLQRV"
     gene            5578..6945
                     /gene="bamD"
                     /locus_tag="VGR03_00565"
     CDS             5578..6945
                     /gene="bamD"
                     /locus_tag="VGR03_00565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEV1992803.1"
                     /translation="MRRIFLILIAALLCLSTGTSYAQKKNKHSKKAGPVDNSAEPDKV
                     LYARAMADMKRGHQEVARLTLQTLINTYPDSEFLAKAKLAIADSYYKEGGTANWAQAI
                     AGYKDFIVFFPFLPEAPYAQLQVAMVHFREMEKPDRDRAHSRAAEDEFQTFLQKYPND
                     PLAPKAEQRLREVQEMLAEGDFRIANFYYIKGSYRASAARLITVTNRYPLFSHSDRAL
                     WMLGTIFEKSERKDLAASSYASIVRNYPLSGLTDSAKRKLTALKVPIPQPDSQALARM
                     QQERSANHRGGGMIHKATGILRTGPDVQTAATVGKPNLEPEPASSSGVDILKPGGKNG
                     PGGSTTGIVATVTPGSAASAPASTGGATSGAESTGGSSGDSSGASPGSSASGDAGAAT
                     SSQPENPNGAATASDKGQAAADSKPADGQGATPKAGDGKASPDDKKKESSSKKKKGLR
                     KIIPW"
     gene            7116..8612
                     /locus_tag="VGR03_00570"
     CDS             7116..8612
                     /locus_tag="VGR03_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV1992804.1"
                     /translation="MAKILVADDNSHIQRMVGLVLKDQGIDVVAVGNGEAAVRKISDM
                     RPDLVLADVFMPVRSGYEVCEFVKSDRSLSHIPVILLVGAFDPLDEQEAQRVGADGVL
                     KKPFVPPEPLISMVKSALSRSSVSSTHGSAPAIAQEVPPRAEKVPSQDIHTPAPQPIK
                     IPEGSTLLAFGSLLDTPTVAPPQRTTAEEEGDPSFLVPRHPSLDEMRDWNAAHTHEAD
                     AEVAEEEDGDSPSGWRRDAVHESLEEQIPAGAVRDWRDAAASGMKHLEDQPVESMDAV
                     APASGSSEAQTVENSAASSDLPVAEIPATVQKPATPVRATAGPEPLKAVGAPEESASG
                     GAPPDSGMAFPAKQEPVPGTKSDSWFSISTSPWDAVVRKAKELAENRNLDSTPAQERP
                     GMTATVFRSTDTHTLPPESTTAIPSAGDRLATPGFEAATPSVADAGMPPQETGPAQAP
                     PAALTTDMEALVTQVLAKMSPDLLQNMTRELLKPIVEAIVRGDLDLKK"
     gene            8771..>11386
                     /locus_tag="VGR03_00575"
     CDS             8771..>11386
                     /locus_tag="VGR03_00575"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV1992805.1"
                     /translation="MTREIAKAYEPQPIETRWAEYWVNEALFRADPASPGPAFSIVIP
                     PPNVTGSLHIGHMLDHTEIDILTRWHRMRGYNALYLPGTDHAGISTQRVVVRQLSEQG
                     IDYRKLGREEFERRVWQWKEESGGTITRQMKQIGESCDWSREKFTLSPDLSRVVREVF
                     VRLYEEGLIYRAHYLINWCPNCLTALSDLETLHEERQGHLWYIRYPVAGTKDHLVVAT
                     TRPETMLGDTAVAVHPDDERYRHLVGKKILLPLTKREIPIIADSMVDREFGTGAVKVT
                     PAHDPNDFELSRRHKLPEIDVMTDDAHMSAAAGAYAGLDRFEARKRVVEDLKAGGLLD
                     KITAHTYSVGTCERCKTVVEPRASTQWFCKMKELAEPAISAVESGEVEIIPANWREVY
                     FNWMRNIRDWCISRQLWWGHRIPAWHCADCKGITVSRETPAKCVKCGSAKIVQDPDVL
                     DTWFSSALWPFSTLGWPEDTPDLRRYYPTSVLITGYEILFFWVARMIMMGIHFTGRIP
                     FRQVYLHSIVRNAEGQKMSKSKGTGIDPIEVNQKYGTDAMRFTLAAMAAPGGDLIWTE
                     DRILSARNFANKIWNAARFLFVNLDKFEQAGAKIEDLAAPEIRAGAPYGSDGQIALAD
                     AWVFSRFARTIELVNAALTNYRFHEAAQAVYQFFWDDFCDWYIEWVKPELTGADRIRA
                     TVAWKNLFAAFDAALRLLHPFMPFLTEELWHQLPQRAEGKSIALERFPEAKREWLQPE
                     VETQVELLQRGIGELRNIRADMKLDPKKKIAAEFSSPDPAIRDLVERNAGAFLRLASL
                     SGLRITTDKLPTAGGTVRSTAQFDVRVAFAEGVDIAAEVGKLRKEVERLEKDIASKER
                     RLADEDFRSKAPGQIV"
BASE COUNT         2327 a         3552 c         3414 g         2093 t
ORIGIN      
        1 gccaggacgg acgcatcccg tgggccttga ccgcggcagg aatctataac cggatccggg
       61 gctttgcccc ctggccggga gcattcacca cattccgcgc acgcctgtgc cagctatggg
      121 gtacgccatc gcccgggaca acgccagcgc cggttgggcg catcttcact gtggatggag
      181 acctcttcgt tgcatgtggc tcggggacac ttcttcgtct ggaaagcgtc aaattagagg
      241 ggcgcaaacg cgtcggcgca caggaatttg catctggcgt gcgcctcaag gatggcgagt
      301 cttttgggcc gtgagagcat ataatcatta agccgcctcg atttgcgcgg ggcaacactc
      361 tcaggcatac tcgcgcttct gtcttagatt tcagaatctg gagcaactga atgaatgatc
      421 atcgtggccg gaatcgccct ttggggcgat ggaccgccgg cagccgcatc cttttgtcct
      481 tcttgctcgc gtttcagtgc tccagtgcac aactcacccg ggcggccgac tcatctgcgc
      541 cgacgaagct ggcgccagac cgcgtactcg aaacgatgaa agccgaattg gcgcgagcga
      601 agaaggagct cgccaaagcg gatcctgcgc cgtacttctt gagctacacc gtgcacgacc
      661 agagcattgt cgtccttgct gccgctttcg ggagcctgtt gaccaatgca tcggcacaac
      721 gccgcgaggc ggatgtcgtt atgcgtgtcg gctctccgct tcttgacaac acacacggtc
      781 agagccgctc ttccggcgta acttccggcg ctctgcccct cggtgacgac acagacgcta
      841 tcgcccgcgt cctctgggag ctcaccgacc gagaatacag acgcgcggct cccgcctacc
      901 tcaatgtaaa gacgaacacc gccgtccggg cggaagaaga agacaagtcg cccgattttt
      961 cgaaggagac atcgcaaacg agtgcggcga agccggccgc tgccccctct ttggaccgcg
     1021 cagcctggga gggcgaaatc cggcgccttt cggcggcttt tcggaagtac ccggaggtct
     1081 actctgcatt cgttgtgctt cagctccaga gctcgacgtc gaggatgttt tcgagcgaag
     1141 gcacggaaat cgtgatcccc agctcgacgg cgcggctcgt catcgacggg cagactcgcg
     1201 ccgaggacgg gatggagctt ctccgtatcg aaacttttca ggcgccgtca ccggctgagc
     1261 tccccgcgga atccgagctc atctccaaaa tcgaaaagat tgcgttggac ttgcaggcgc
     1321 tgcgcaaagc gcctgtcgcc gaaccctatg acggtccggc cgtgctttcc ggccgcgcgg
     1381 ctgcggtctt ctttcatgaa gtgctcgggc acaggctgga aggccaccgt caacgggatg
     1441 aagaggaagg acagaccttt acaaaaaaag tcggcctgga ggtcctgccc aagttcctga
     1501 gcgtcgccga tgacccgacg gtgcgtcaga tccatggcac gaaactggcc ggctcgtatg
     1561 attttgacga cgagggcgtg gcttcccgcc gcgtgcaagt cattcaagac ggggtgctca
     1621 agagtttcct catgtcccgc atgcccatca agggctttga aaactcgaat ggtcatgggc
     1681 ggaaccagcc cggactgatg ccgaccggcc gccaggggaa tttgatcgtc acttcgacgc
     1741 agaccgcctc agacgcggac ttgcgcaagc gcctcctcga agaggtcaag aaacagggaa
     1801 aaccctacgg gctttacttc gaagacgtgc agggcggctt tacgctcacg acgcgttcgt
     1861 tgccacaggc gttccaggtg cttcccgtca tggtctatcg cgtctatccg gatggcaggc
     1921 cggatgagct ggtgcgcggc gttgacatcg tcggcacccc gctggcggcc ctcactcgca
     1981 ttctcttgac aggagacaaa gaacaaatat tcaacggcgt gtgcggcgcg gaatccggca
     2041 gcgttccggt atccgcagtg gctccagcca tgctcttctc agagatggaa gttcaaaagc
     2101 gcgcacactc gcacacgcgg ccacccatcc tcccgcctcc gggcttcgaa accacgcctg
     2161 ccaccccaga tgcgaagccg accccggggg tgaagccgtg agagctgcca ggtttcttct
     2221 cgcggtcttg agcctcgctc ccctggctct tctcacttcc acatcgctct gcgcctcgac
     2281 cgatgacgcc gctgggaaat cggacccggc ggcgaaggcc cgcgctgccg cagcaggcga
     2341 cccactcctc gaggcgctgc tgaccgagct tgagcgttcc aagtcgcaat tgaagatgga
     2401 ccaggtccag cccccttatt acatcgaata tcgcgtccac gaagtgaact tgtttgccgt
     2461 cgaggccgcc ttcggagcgc tgcgcgaaga acagaacact cgcgcacgcg tcctccgtgt
     2521 ggtcgtccga gtcggcaact acaagcagga cagtttcttc gggcaaggaa tgggggagac
     2581 cacaatcctt ccgctcgaca acgattccat tgcgattcgc caccagatct ggctggcgac
     2641 cgacgaagcc tacaaggccg ccagtgaagc cctcaccgag aagcaggcgt tcatgaagca
     2701 gtttgcagcg gattcaaatc cggtggacga cttcgcccgg gccacaccca tcaactccat
     2761 tggcagcctc gcgcagcttc gaatcgattc ggcagcgtgg cggaaaacgc ttgaggaatc
     2821 caccaacttg tatcgccaat acccagcatt gcaatcctta tccgcttcgg cccgcttcgc
     2881 cgtggtcaac gaatacttcg tcaactccga ggggacggtc acgcggagcg ggcatgccag
     2941 ttatacggtg acgctcgacg gtgcgacgca ggctgccgac ggcatgcgcc tcaaccgcag
     3001 tccctattgg accgtggccc gcccggaaga gctccccggc cgcgacgctc ttctcggcga
     3061 gacgaggaag atgctcgacg ccctcatggc tctccgggaa gcccccattg cggaggagga
     3121 ataccgcggg ccggtgcttt tctcggcgga tgccgcagac gacatgttcg cggggctcat
     3181 cggcgcgaac atccttggcc gaaaacccca gcccgggaga ccgaaccgta cgaccggcgc
     3241 atttgccacg agcttcaaga gccgggttct gccgtctttt ctgaccgtgg tggatgatcc
     3301 gaccgtgaag cagttccatg gcgagagcct cgtcggcgcc tatgatgtgg atagcgaggg
     3361 agtccttgga caggccatcg tcgcgattga caacggtaat ctgacgaatt atctggtcgg
     3421 ccggcagccg attcgcgatt atcccgcttc gaacggtcac ggccgcgccg ctcccggagt
     3481 cttcccgcag ccgagcctcg gcaatctttt cgtgcgcact tccgaaccga aacccgtggc
     3541 ggctctgcgg caacggatgc tcgagatggt gcgcgctcag ggcaagccgt acgcctaccg
     3601 cgtcgagact cttggtccgg gcaactcgcc ccggctgctc taccgcgtac acgaaaagga
     3661 cggccacgaa gagctcgttc gaggcgccgt cttcaacgaa ctggatgtgc gcaccctgcg
     3721 caacaatttg gcggctgcgg gagacgaccc gctggtgagc aatcgcgccg gcggcgtgct
     3781 gaccacgatc gtcagcccgt cgattctcct cgacgaactc gaagtcaaac gagccgatac
     3841 cagcaaggat aagcttcccg aataccctcc cccgccgctg gcgcaaacga atgccaagaa
     3901 gccatgacca cgcctgacga tcctagttcg ctgcccgaaa ctcccgagat aaccctgcgc
     3961 gagagactcg aacgcgtaag ccggatggcg ctgctcgtgt tcatccttgc ctccgcggca
     4021 ttcctcagcg ccatcacgac gatgcgcatc gctatccacg gccgcgaggt gaccatgccg
     4081 aatctcgtgg gcaagggcgt tgcggaagcg aacgggcacg tgacctccct gggacttgtt
     4141 ctccgcgtcg ccgaccgcgt gtacagcgat ctgcccatga acgtcgtcgt gcggcaaacg
     4201 ccaccgccgg gaatgctcat gaaggtgtcc caagaggcgc acgtcgtttt gagccttgga
     4261 caacggaatc tcacgattcc gcccctggaa ggcaaatccc tgcgcgcttc gcggatcgag
     4321 ctccttcgca cgggacttca gattggagag gtttccgcgc ttgccctgcc cgatgcggcc
     4381 ccggatacgg tagtcattca gaacccgcgg ccaggaagca ccgcagcaac cccgcgcgta
     4441 gacgtgctcg tagtgcagcc accccgtgag aatgccttcg tgatgccgca cctcgtcggc
     4501 ctgagcgaag ccgaggctca gcgcaggctg gaccagtctt ctgttcatca caaagagaac
     4561 tacatggccg ctccgcaatg gccacgcgga gtggtcatag accagacgcc tgtagccggc
     4621 gcgcgccttg cggcttcctc tacggtggaa ctaacagtgg cgaattagag ggggttattc
     4681 actcagcgcg aacacccctg gggcctgctg cagttctgac gggacgactc catgagccgt
     4741 tccgtcaccc aagcaatcgt cttcgcctcc gcgtcccttg cggcccttcg ggcccggaga
     4801 tggggatgtg ttagaatccc ggtgtgaagt ctgcgactcc ttccgtcgaa atagccccct
     4861 ccattctcgc tgcggatttc tcgtcgctcg gcgaggccgt gcgtgcggtc gagcgcgccg
     4921 gcgttaaact gatccacgtg gacgtgatgg acggccactt cgtccctaat atttccattg
     4981 gcgcgccggt tgtcgcctct cttcgcaaag caacccaatt gatactcgac gtgcatctga
     5041 tgattgagag gcccgaggag tatatcgagg cgttcgccga agctggagcc aactggatca
     5101 ccgtgcatca ggaagcgaca gtccacttga accgtgccgt gcatcagatc cacgaagtcg
     5161 gttgccgtgc cggggtggcg atcaacccgg ccacaccggt cagcgcgctc gcggacattc
     5221 ttcctgacgt ggatctggtt ctggtgatgt cggtcaaccc cggttttggg ggccagcact
     5281 tcttgccggc ctcgacgaaa aaaatcgccg agcttcggga actgcgttcg cgctatactc
     5341 ataatttccg gatcgaggtg gatggaggga tagcggccga caccgtccag ggtgtcgttc
     5401 gggccggggc cgagattctg gtggcaggca cctccgtgtt tcacaaggag gaccccgccg
     5461 cggccgtgca cgaactgcag cggatcgccg cggaggctgt cttgcaacgg gtttgacgct
     5521 gccgaattcc gtttgccggc cgcagtccag cccgactttt gggggagagg tgaggtcttg
     5581 cgtcggattt ttcttatctt gatcgctgcg ctgctctgcc tcagcaccgg caccagctac
     5641 gcacaaaaga agaacaaaca ctccaagaaa gccgggccgg tggataattc cgccgagccg
     5701 gacaaggtgc tttacgcgcg ggccatggct gacatgaaac gcggccatca ggaggttgcc
     5761 cgcctcacat tgcaaacgct gatcaacacc tacccggaca gcgagttcct ggcaaaggcc
     5821 aagctcgcca tcgccgattc ctattacaaa gagggcggaa ccgcgaactg ggcccaggcc
     5881 attgccggct acaaggattt catcgtcttt ttcccgttcc tgccggaagc tccctacgcg
     5941 caattgcaag tggccatggt gcatttccga gagatggaaa agcccgaccg ggaccgcgcc
     6001 cactcccgcg ccgcggagga cgagttccag acgttcctgc agaaataccc gaacgatcct
     6061 cttgctccga aggccgaaca gcggctacgc gaagtccagg aaatgcttgc cgaaggcgat
     6121 tttcgaatcg cgaatttcta ctacatcaag ggatcctacc gcgcgtctgc cgcgcgcctg
     6181 atcaccgtca caaaccgcta tcccctgttt agccattcgg accgcgcgct gtggatgctc
     6241 ggtacgattt tcgaaaagag tgaacgcaag gacctcgccg ccagctccta cgcaagcatc
     6301 gtgcgcaatt accccctctc cgggctcacc gacagtgcga agagaaagct gactgcgctg
     6361 aaggtaccca tcccgcaacc ggattcacag gcactggccc ggatgcaaca ggaacggagc
     6421 gcaaaccatc gcggcggagg gatgatccac aaggcgaccg gaatactccg gacaggcccc
     6481 gacgtgcaga cggctgccac ggtgggcaag cccaatcttg aacccgaacc ggcttcatcg
     6541 tcgggggtag acatcttgaa acccggggga aagaacggtc ccggggggtc cacgaccggt
     6601 attgtcgcga ctgtgacccc ggggtcggcg gcgagcgcac cggccagcac gggcggcgca
     6661 acctcgggcg ctgaatcaac cggtggatct tcaggggatt catccggggc aagtcccggg
     6721 agttcagcga gcggcgatgc aggcgcggcc acgagctcgc aacccgagaa tccgaatggc
     6781 gccgcgacgg catcggacaa gggccaggct gccgccgatt caaagcccgc ggatggacag
     6841 ggtgcaactc ccaaggcggg cgatggcaag gcttcgcccg acgacaagaa gaaagaatcc
     6901 tcgagcaaga aaaagaaagg gctcagaaaa atcattccct ggtagccggt ttcagcgtca
     6961 tctggcctga ttcggcacgg cgcagacgcg gccgttgcct ggcataggcc gttcggacac
     7021 acgcaatcgg ccttttcgtt gacgctccct ccaagcgatg ctccaatgaa tacaaagaat
     7081 tatcgggtat tggtgtctga cccggagggt gtcctgtggc taagattttg gtcgctgacg
     7141 acaacagcca tattcagaga atggtcggcc tggtattgaa agaccagggc atcgacgtcg
     7201 ttgcggtcgg caatggcgaa gccgctgtac ggaagatttc cgatatgcgg cctgacctcg
     7261 ttctggccga tgtttttatg cccgttcgaa gtggctacga agtctgcgaa ttcgtaaagt
     7321 cggatcgcag cctctctcac atccccgtca tcttgctggt gggcgctttt gatcccctgg
     7381 atgaacagga agcgcagcgc gtcggcgccg acggggttct gaagaagccc ttcgtccccc
     7441 cggaaccgtt gatctccatg gtgaaatctg cgctctcgcg gtccagcgtt tcctcaaccc
     7501 acggatccgc gcccgcaatt gcgcaggaag tgccgccgcg cgcagagaaa gtaccgtcgc
     7561 aggacattca taccccggcc cctcagccca tcaaaattcc ggaagggtca acactgctcg
     7621 cgttcggaag ccttctcgat actccgaccg tcgccccgcc tcaacgaacg actgcagagg
     7681 aagaaggaga tcccagcttt ctggtgccgc gccatcctag cctcgacgag atgcgcgatt
     7741 ggaacgccgc ccatacccac gaagctgacg cggaggttgc ggaagaagaa gacggcgatt
     7801 ccccatccgg ctggcggcgg gacgcagtgc acgaatcgct ggaggaacag attccagcag
     7861 gggcggttcg cgactggcgc gatgctgctg ctagcggaat gaaacactta gaggaccagc
     7921 cggtggaatc tatggacgcc gtcgctccag caagcggctc ctctgaagcg caaacggtgg
     7981 aaaattccgc ggcttccagc gaccttccgg tagctgagat acccgcaact gtccagaagc
     8041 ctgcaacgcc cgtgcgtgcc accgcagggc ccgagccact gaaagccgta ggagcacccg
     8101 aggaatctgc cagcggagga gctcctccgg actcgggaat ggccttccct gccaaacaag
     8161 aacctgtccc tgggacgaaa tccgattcct ggttctccat ttcgacgtca ccctgggacg
     8221 cggtagtgcg gaaggcaaaa gagctcgccg aaaacaggaa tctggactcc actcctgcac
     8281 aagagagacc cgggatgacg gccacggtat tccgcagcac ggatactcac accctgcctc
     8341 cggaatcgac cacagcgatc ccctctgccg gcgaccggct cgcgacgcct ggcttcgagg
     8401 ccgcgactcc ttccgtcgct gatgcgggca tgcctccgca ggaaactggc ccagcccaag
     8461 ccccgcctgc ggccctcaca acggacatgg aagccctcgt aacgcaagtc ctcgcgaaga
     8521 tgtcgccgga cctgctccag aatatgaccc gcgagctctt gaaacctatc gtcgaagcca
     8581 tcgtccgcgg tgatcttgat ctgaaaaaat agcgtctctg ttttcctccc ccctttttgt
     8641 tctttgccgg cacacctgtc tgcagattga cgcgtgcgcg ttttcgccct tatagtcagg
     8701 ttttggcctc acccagttgc cgtgcgaggg ctgcacggat tcgcataaaa ggcgggctgg
     8761 aaacgtcctt atgacgcgag aaatcgcaaa agcctatgag ccgcaaccca tcgaaacgcg
     8821 ctgggcggaa tactgggtga acgaggcact ttttcgcgcc gaccctgcct ctccgggacc
     8881 cgcattttcc atcgtgattc cgccaccgaa cgtgaccggg tcgctgcaca tcggccacat
     8941 gcttgatcac acggaaatcg acatccttac gcgctggcac aggatgcgcg gttacaacgc
     9001 cctttacctg cccgggacgg accacgccgg aatctccact cagcgcgtcg ttgtccgcca
     9061 gctcagtgaa caagggatcg actatcgaaa gcttggccgc gaagaattcg aacggcgcgt
     9121 ctggcagtgg aaagaggaat ccggcggaac gatcacccgc cagatgaaac aaataggcga
     9181 atcctgcgac tggtcacggg agaaattcac gctttccccc gacctttccc gtgtggtccg
     9241 cgaagttttt gtgcgccttt acgaagaggg cctcatctac cgcgcgcact acctgatcaa
     9301 ctggtgcccg aactgcctga cggccttgag cgacctcgaa acccttcatg aggagcgcca
     9361 gggacatttg tggtacatcc ggtacccggt ggccggaacg aaggatcatt tggtcgtggc
     9421 cacgacgcgc ccggagacca tgctgggcga tacagcggtg gccgtccatc cggatgacga
     9481 gcggtacagg cacctcgtcg gcaagaaaat tctcttaccg ctcacgaagc gcgagattcc
     9541 catcatcgcg gactccatgg tggaccgcga gttcggcacc ggcgcggtga aagtgacccc
     9601 ggctcacgac cccaatgatt tcgaactgag ccgccgccac aagctaccgg aaatcgatgt
     9661 gatgaccgac gacgcccaca tgagcgcggc ggctggggcc tacgccgggc tcgaccgctt
     9721 cgaagcacga aagcgcgtcg tggaggacct gaaggcaggg gggttgctcg acaagattac
     9781 ggctcacact tattcggtcg ggacttgcga gcggtgcaag accgtggtcg agccgcgcgc
     9841 ttccacgcag tggttctgca aaatgaagga gcttgcggaa ccggccatct cggctgtgga
     9901 gtccggtgag gtcgagatca tcccggcgaa ctggcgcgag gtctatttca actggatgcg
     9961 gaacatccgc gactggtgca tctcccgcca gctctggtgg ggccaccgca tcccggcctg
    10021 gcattgcgcc gattgcaaag gcattacggt gtcgcgggaa acgccggcga agtgcgttaa
    10081 gtgcggctcg gcgaaaatcg tgcaggaccc cgacgttctc gatacctggt tcagctcggc
    10141 gttgtggcca ttctcgacgc tcggctggcc ggaagacacg ccggacctgc gccggtacta
    10201 tccgacgagc gtcctgatta ccgggtacga aatcctcttc ttctgggtcg cgcgcatgat
    10261 catgatggga attcacttca cggggaggat cccctttcgg caggtctatc tgcactcgat
    10321 cgtgcgcaac gccgaaggcc agaaaatgtc gaagtccaag gggaccggca tcgacccgat
    10381 cgaggtgaac cagaagtacg gcacggacgc catgcgcttc acgctggcgg ccatggccgc
    10441 gccgggcggg gacctgattt ggaccgagga tcgcatcctg agcgcaagga actttgcaaa
    10501 taagatctgg aacgcggcgc ggttcctgtt cgtgaatctc gacaagttcg agcaagcggg
    10561 agcaaagatc gaagatctgg ccgcgccgga gattcgcgcg ggcgcgcctt acgggagcga
    10621 tggccagatc gccctggccg atgcgtgggt cttctcacgc ttcgcgcgca cgatcgaact
    10681 ggtgaacgcg gcgttgacga actaccgctt ccatgaagcc gctcaggccg tatatcagtt
    10741 tttctgggac gatttttgcg actggtacat tgaatgggta aagccggaat tgacgggagc
    10801 ggaccggata cgcgcgacgg tagcctggaa gaacttgttc gcggcgtttg acgcagcact
    10861 gcgcctcctg cacccgttta tgccgttcct gaccgaagag ctttggcatc agctgccgca
    10921 gcgcgcggaa gggaaatcca ttgcgctcga aagattcccg gaggccaagc gggagtggct
    10981 ccagccggaa gtcgaaacgc aggttgaact cctgcaaaga ggcattggag agctgcgcaa
    11041 catccgcgcg gacatgaagc tcgacccgaa gaagaagatt gcggcggagt tctctagccc
    11101 cgacccggcg atccgggacc tggttgaacg caacgccggc gcattcttgc ggctggcgag
    11161 tctatcgggt ttgcggatta cgacggataa acttccaacg gcgggcggca cggttcgctc
    11221 gacggcacag ttcgacgtgc gggtggcgtt tgcagaaggg gttgacatcg ccgcagaggt
    11281 cggaaaactc cgaaaagagg tcgagcgcct ggaaaaggat atcgcatcga aggagcggcg
    11341 gcttgcggat gaagactttc gaagcaaggc ccccggacaa atcgtt
//
LOCUS       DASYVO010000013         5762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_225785, whole genome shotgun sequence.
ACCESSION   DASYVO010000013 DASYVO010000000
VERSION     DASYVO010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225785"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..985)
                     /locus_tag="VGR03_00580"
     CDS             complement(215..985)
                     /locus_tag="VGR03_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEV1992806.1"
                     /translation="MAEAAQTSPTVASRQPSHSYLVFLLQVSRPGLWSTTAMFYLMPL
                     GHADFLHTGKLWLGLFFVLFPLSFLLYGVNDLVDAETDALNPRKGTFMFGSRGAREQL
                     SALKWQIAVVQLPFAVSFYFFVGPRILWWYALLLLAVGLYNAPGIAWKGRPPFDVLIQ
                     ASYLLVFVLSSWLNGVAQLPWQTFLFGALFAMHSHIFGEVMDVEPDRLSGRRTTATLI
                     GRVRAKFLIATFLCIETALVWIYFRDWLITGFLAFGAL"
     gene            complement(1125..1676)
                     /locus_tag="VGR03_00585"
     CDS             complement(1125..1676)
                     /locus_tag="VGR03_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1992807.1"
                     /translation="MAVARNLWGYIDRRDHRLMRRMNRWRAPRWIRIWMIAATRMGDG
                     WIWYGLGLMLLAYGGPQRFSAIGAAGSAAVLGIFVFKALKRLSQRPRPCQIEPHCWSK
                     VLPPDQFSFPSGHTMTAFSIALVISYFYPALEGVLFFMALSIAVSRIVLGMHFLSDVL
                     AGVVMGVALGCASITAFASFGLI"
     gene            complement(1835..2788)
                     /locus_tag="VGR03_00590"
     CDS             complement(1835..2788)
                     /locus_tag="VGR03_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HEV1992808.1"
                     /translation="MDTFTQVSPPLYRLFISGVDNCTMRCDLHVHTIASGMFNAPGLD
                     RICRESYNDPAEVYERLKRMSMSIVTITDHDSIEGAEALRRHPDFFLSEEVTVRMPSG
                     TEMHMGVYGITERDHVEIQRRRNDFIALLMYLTERKLFFSANHVFSGLTGRREEEDFH
                     WFESYVPAFETRNGQMWPEANESAARLAARLGKIAIAGSDSHTIAGVGHTYTEAPGAR
                     TVDEFFAGLRTGRGRIHGTHGSYGKITRDVFSITRSLFGDRPWTLAASPLALLVPAFT
                     AGHWLNEIRFCRKWSAAIERGEKRKRMFWDLDSNFEANWAS"
     gene            2883..4070
                     /locus_tag="VGR03_00595"
     CDS             2883..4070
                     /locus_tag="VGR03_00595"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1992809.1"
                     /translation="MKRVHVVFHDGGGGHRNAAVALQTVISQQQRDWQIELIQFQDLT
                     DRLDVLRKLTGIRIQQQYNILLQNGWTLGSTQLLRILQTTIRLFHRPLVNLLETFWRE
                     KPADLLISVIPHFNRQICESWTKVYPGRPFVTIITDLADFPPRFWIEPIREQHVIAGT
                     EKAAEQARAMGHDEAHIFLTSGMILRPDFYVQDNSDPVALRREMGLREDLPTAIVLFG
                     GHGSKVMYGITEKLDAAALRLQLILICGRNEELAGKLRARQWKLPVHVVGFTKEVHKL
                     MRAADFLIGKPGPGSIAEAMVRKLPVLIECNARTLPQERYNAEWVKEKGVGIVLKSFR
                     SVADGVKRMLDPTALAEFRKNVTAQENRAVFEIPEILAKLLGEPNSTTGSPALAPVET
                     KAH"
     gene            complement(4185..4517)
                     /locus_tag="VGR03_00600"
     CDS             complement(4185..4517)
                     /locus_tag="VGR03_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992810.1"
                     /translation="MRRLFSKAISWRRPIFRLYLLGIIFLACSLTLLADTPALLRSIP
                     AGGCYCHCAESHLRGGCVKLCDSKRYAARWRGTKCAKPHMQTPADNSNAGPRFPRPGR
                     AEHAQLHQ"
     gene            complement(5104..5325)
                     /locus_tag="VGR03_00605"
     CDS             complement(5104..5325)
                     /locus_tag="VGR03_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992811.1"
                     /translation="MTLVEITYELQSPLTEEQLRQLGQFANTYGLRSFRVDEATNQLS
                     FEYDASRLRETQVAHVLGQARIAVARKVN"
     gene            5426..>5762
                     /locus_tag="VGR03_00610"
     CDS             5426..>5762
                     /locus_tag="VGR03_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV1992812.1"
                     /translation="MAVKARKATRSARQKNRHESSANCACDAGADVRKAIGVDPGLKE
                     ANQNRLRRIEGQVRGLQKMVEEDRYCADIITQVASVQEALRSVARNLMKNHLHQCAAK
                     ALKSGKKEET"
BASE COUNT         1397 a         1616 c         1661 g         1088 t
ORIGIN      
        1 attgccgctt caagagtctc aacactgatt tcgagcaggc atttccggtt catctccgaa
       61 gtcctgcggt ggatacgaca tgcgtaagcg tcccgtgggt ccaattccag aacattccca
      121 gcagggcggc ggcgttccaa ccccacaaga aaagccgcat ttctcttgga cgataagcgc
      181 ggtctttcca caataaggtc gcatcgatca agaatcacag cgcgccgaag gccaggaagc
      241 ccgtgatgag ccagtcgcga aaatagatcc acactagggc agtttcaatg cacaaaaaag
      301 ttgcaatcaa gaatttggcg cgtactctcc cgatcaaagt cgctgtcgtt cgccgtccac
      361 taagccggtc gggttcgacg tccatcacct caccaaaaat atgggagtgc atggcaaata
      421 acgcgccgaa cagaaaagtc tgccagggaa gttgcgcgac gccgttcagc caactgctca
      481 aaacgaatac gagcaagtaa ctcgcctgaa tcaacacatc gaacggaggg cggcctttcc
      541 atgcaatccc gggcgcattg tacaggccga cggccagtaa gagcagggcg taccaccaca
      601 aaatgcgcgg cccgacgaag aaatagaacg acaccgcaaa ggggagttgc accacggcaa
      661 tctgccattt caaagcgctg agctgttctc gtgcgccgcg cgagccgaac atgaacgtcc
      721 ctttgcgcgg gttcaacgca tcggtctcgg cgtcaacgag gtcgttgacg ccatagagca
      781 gaaaactgag agggaaaagc acaaaaaaca atcccaacca gagtttgccg gtatgtaaga
      841 aatccgcatg cccgagcggc atgaggtaaa acatggctgt agtgctccac agcccgggcc
      901 gtgaaacctg tagcaagaaa accaaatagg aatgagacgg ttgacgactt gcgacagttg
      961 gagaggtttg ggcggcttcg gccatctggc ccaatatacc gcggttccca ccgcgcctgg
     1021 gtcttcaaac tttcaaaatg tgatgaatcg tttcggccgt ttcaaattct tcgcgcaata
     1081 agaatgtgaa gaagacgccg acgggcaagt atatccggtg cgcgctagat gaggccgaac
     1141 gaggcgaacg ccgtgatcga agcgcacccc agggcaacgc ccatgacaac acccgccagg
     1201 acatcactca ggaaatgcat gccgagcacg atgcgggaga ccgcaatgct cagtgccatg
     1261 aagaaaagca cgccttcgag cgcgggataa aagtagctga tgaccagcgc gatggaaaac
     1321 gcggtcatcg tgtgacccga tggaaacgaa aactgatccg gaggcagcac cttcgaccag
     1381 cagtgcggct cgatctggca aggcctcggc cgctggctca gtctcttcag tgctttgaaa
     1441 acaaaaatgc ccagaaccgc ggcagacccc gcggcgccga tggccgaaaa acgctgcggg
     1501 cctccatacg cgagcaacat cagacccaga ccgtaccaga tccagccatc gcccatgcgc
     1561 gtggcggcaa tcatccagat gcgaatccag cgcggcgcgc gccagcgatt catccgccgc
     1621 atcagccggt gatcgcgccg atcgatatat ccccagaggt tgcgtgcgac tgccatcgct
     1681 tcacctcacc caaccggccg gaaacaagca agcgctttca acgagaaaga tgcacaaata
     1741 ggcgaaaaag acctctcgag cacaaatcgc gtgcgcatgc aacgatcttc gcctcgcttg
     1801 cagtgacgga ttgggggact cgctcacgcg cacctcaact tgcccagttg gcctcgaagt
     1861 ttgaatccaa atcccaaaac atccgcttgc gtttctcacc gcgctcgatt gctgctgacc
     1921 atttccggca aaaacgaatt tcgttcaacc agtgccccgc agtaaatgcg ggtaccagca
     1981 gcgcgagcgg cgacgctgcc agcgtccacg gtctgtcccc aaaaagtgat cgggtaatac
     2041 tgaacacgtc tctcgtgatt ttgccgtagc tcccgtgcgt gccgtggatt cgtccgcggc
     2101 ccgtccgcaa ccccgcgaaa aattcatcca ccgttcgcgc cccgggcgct tccgtgtaag
     2161 tgtgtcccac acccgcgatg gtgtgcgagt cgctgccggc gatcgcaatt ttccccaacc
     2221 gtgccgccag ccgcgccgcg ctctcattgg cctccggcca catctgcccg ttgcgtgttt
     2281 caaacgccgg aacataggat tcaaaccaat ggaaatcttc ctcttcgcgg cggcccgtca
     2341 acccggagaa aacatgattg gcactgaaaa agagtttccg ctcggtgagg tacatgagca
     2401 gagcgatgaa atcgtttctg cgcctctgaa tctccacgtg gtcgcgttcg gtgatgccat
     2461 agacgcccat gtgcatctcg gtgccgctgg gcatccggac ggtcacctcc tcgctcagga
     2521 aaaagtccgg gtggcgccgc agggcctccg cgccttcgat ggagtcatga tcggtgatgg
     2581 tgacgatgga catgctcatc cgcttcagcc gttcgtagac ttcggcggga tcgttgtagg
     2641 actcccggca aatgcggtcc agcccgggag cgttgaacat tccggatgcg atcgtgtgca
     2701 catgaaggtc gcagcgcatg gtgcaattgt ctacccctga tatgaacagc ctgtaaagag
     2761 gtggcgaaac ctgcgtgaat gtgtccatgg aacttattga acacaaatag tttattggct
     2821 tatccacatg gcatggagtg tgctctggtg agacggcccg aacttatggc acaatcggcg
     2881 caatgaaaag agttcacgtc gtcttccatg atggcggagg ggggcatcgc aacgccgccg
     2941 tcgcactaca gacggtaatt tctcagcagc aacgcgactg gcagatcgag ctcatccagt
     3001 ttcaggactt gaccgaccgg ttggacgttc tgcgcaaact gacaggcatt cgcatccagc
     3061 agcagtacaa catcctgttg caaaacggct ggacgctcgg cagcactcaa cttctccgca
     3121 ttcttcagac cacgattcgc ctatttcaca ggccgctagt gaatctgcta gaaacatttt
     3181 ggcgcgaaaa gcccgcggac ctgctcatct ccgtaattcc ccatttcaac cggcagattt
     3241 gcgaaagctg gaccaaggtt tatcccggcc ggccgtttgt caccatcatc accgacctgg
     3301 ctgattttcc gccgcgattc tggatcgagc cgatccgtga acaacatgtt attgcgggca
     3361 cggaaaaagc ggccgagcaa gcgcgcgcca tgggccatga cgaggctcac atttttctga
     3421 catccggaat gatcttgcgc ccggattttt atgtgcaaga caattccgat cccgtggcgc
     3481 tgcgccggga aatgggactg cgcgaagatc tgccgacagc cattgtgctc tttggcgggc
     3541 acggctcgaa agtgatgtac ggcattacgg aaaaactgga tgcggccgcg ctgcgtctgc
     3601 agttgatctt gatttgcggc agaaacgaag aactggcggg gaaattacgc gcgcgccaat
     3661 ggaagttgcc ggtgcacgtc gtcggcttca cgaaagaagt gcataagctg atgcgcgcag
     3721 ctgatttttt gatcggcaaa cccggtccag ggagcatcgc ggaagcgatg gtccgcaagc
     3781 tcccagtgct gatcgaatgc aacgcccgga ctctcccgca ggagcgctac aacgcggagt
     3841 gggtgaagga gaaaggcgtc ggcatcgtat tgaagagttt tagaagtgtg gcggacggag
     3901 tgaagaggat gctcgatcct acagcgctcg cggagtttcg caagaatgtg acagctcagg
     3961 agaaccgcgc cgttttcgaa atccccgaga ttctcgcgaa gctcctcggt gagccgaact
     4021 caacgaccgg cagccccgcc ttggcgccgg tggaaaccaa agctcactaa catttaagca
     4081 gacagaacga atagcgcgta tctgaagacc cgagctgaaa actcaaaact gaaaactcaa
     4141 aactcaaaac tcaaaactga aaactgaaaa ctgaaaacac ccagctactg gtggagttgc
     4201 gcatgctccg ctcggccagg acgcgggaat ctcgggccgg cgttggagtt atcggccggc
     4261 gtctgcatgt gtggcttcgc gcacttcgtt ccgcgccagc gtgcggcata tcgcttggaa
     4321 tcgcaaagct tcacacagcc gcctcgcaag tgcgattccg cacaatggca gtagcagccg
     4381 cccgcgggaa tactccgcag caatgcgggc gtgtccgcga gaagcgtgag tgaacaggcc
     4441 aggaaaatga ttcccaggag gtagagtctg aagatgggcc gccgccacga gatggctttg
     4501 ctgaagagtc ggcgcatggg ggtattctgc ggagagcgcg ggaaaaaacc gatagccctg
     4561 tgagttcgaa aattttgtta ccagggtcct ggaaccaggt ggaaggggcg cgcccgaagg
     4621 cgatggagag aggccgttcc cgattcagac tggagacagt tccgaattga actaggagta
     4681 cggatttggc gcaggtggtt aagcgctcct tacgactaac ctcaacataa taagatactt
     4741 atctaactag gagcaaattc tacattggag ggagccgtgc attagacggt tgcgtgggcg
     4801 cgtttcaaat gacgagatgt gaagagagac aagcgagggg gaaggggctt gcttgtcact
     4861 ttttacagat cgttcccacc tgcctctgag agggaagcta ggatggatga ccccacgcgc
     4921 tggtgtgaaa acgacgccag caattgaaac gcgccttctg gggcccccga tcccaagcgg
     4981 gggccccttt tttttgtagc tccgatgccg aactccactg cgattggtta atcggacggc
     5041 caacccggca tcgagttcac ggtcgcgcct gagcatcctt caaactagcc agaaaaaatc
     5101 gggtcaattc actttgcggg caacggcgat gcgcgcctgg cccagcacat gcgcgacctg
     5161 cgtctcgcgc aggcgtgaag cgtcgtattc gaatgacaat tggttcgtcg cttcgtcgac
     5221 acggaaactg cgcaatccat aggtgttcgc gaattggccg agctgccgca attgctcttc
     5281 tgtgagcgga gactgcaatt cgtaagtgat ttccacgagt gtcatggcgg cattatacca
     5341 gcccacggcg agcgggaggc tgtcacggct gcggccgttt gacacaagat agggggtagg
     5401 ggtataaata gggggggagg gtatagtggc ggtcaaggct aggaaggcga cgcggagcgc
     5461 caggcagaag aatcggcacg aatctagtgc gaactgcgcc tgcgacgccg gggccgacgt
     5521 acgcaaagcc atcggggtcg atcccggtct gaaggaggcc aatcagaacc gcctccgccg
     5581 catcgagggg caggtccggg gtttgcaaaa aatggtggaa gaagaccgct actgcgcgga
     5641 catcatcacg caggtggctt ccgtgcagga ggcgttgcgt agcgtcgccc gcaacttaat
     5701 gaagaaccat ctgcatcagt gtgcggccaa agcgctgaag agcgggaaga aagaagaaac
     5761 cg
//
LOCUS       DASYVO010000014         8224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_99003, whole genome shotgun sequence.
ACCESSION   DASYVO010000014 DASYVO010000000
VERSION     DASYVO010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8224
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99003"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VGR03_00615"
     CDS             <1..1243
                     /locus_tag="VGR03_00615"
                     /inference="COORDINATES: protein motif:HMM:NF015836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992813.1"
                     /translation="ARGPGYPAFLAGIYFLAGTGRQAVMLAQAFVDLATCVLAAGIAH
                     RLAAGAPDATRTRVAAAALWLTALCPFTANYAAVPLTEVLATFFTTLALLIFLSPAAT
                     EIDRIASNPDLLRATKIWFAGGLVIGLGTLVRPETPLLLGAVLIALWLRYRRPANWKR
                     LALATLWMIVGLLLPLAPWAARNSISVGRAQFLAPRYAETYGDVLPTGFYAWTKTWMF
                     RFRDAYLFTWKLPMQPIDLKNLPSYAVDSPEELSRVTSLLDRYNRARGMTRSLDLEFA
                     ELAQKRAKRHPIRTYVWIPVERAAAMWFTPRITLLPYSGRLWPLTESYRSNPTDFEVT
                     LGLALLSVLYVGIGLAAAWSSRTNPGVSLIVAFILIRTAFLTQLQTCEPRYVLVCFPA
                     LLAVGAQLWRHSKTGREIVIA"
     gene            1395..3035
                     /locus_tag="VGR03_00620"
     CDS             1395..3035
                     /locus_tag="VGR03_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEV1992814.1"
                     /translation="MTWTQVYDPLGHWWLSTLVATLPVVVLLGALAVLRIKAHWAALL
                     GLASSLLIAVGIFKMPGAMAARTAVFGAGYGLFPIGWIILNVIFMYDMNCASGRFKVL
                     QESLMGITQDRRLQLLLIAFSFGAFFEGAAGFGTPVAVTAAILIGLGFKPLQASGLSL
                     IANTAPVAYGALGTPLVALQAVTGLDLLQLSAMAGRILPFFSVLVPFWLLWAFAGFSG
                     MLEIWPAVLVAGVFFAVPQFLVSNFHGPWLVDVVSAVCSMAAVIVLLLLWHPKKIWGF
                     EGHESDRSARGQHGHSAGAVAKAWVPWVILSVLVFLWGLPSVKTLLDKISIFKIPVSG
                     LHNLILRVPPVVAKSTPEAAVFNFNWLSATGSGILIAAVISGFVMGFGPLQMLRQYGK
                     TLYKVRFSLITIAAMMSIGFMTRYSGLDATMGLAFARTGTLYPFFGTMLGWLGVALTG
                     SDTSSNVLFGSLQKISATQVGVSPTLMAAANSCGGVMGKMIDAQSIVVASTATEWYGH
                     EGDILRYVFFHSIALACLVGVLVFLEAYVFPFTKMVIH"
     gene            3263..4711
                     /locus_tag="VGR03_00625"
     CDS             3263..4711
                     /locus_tag="VGR03_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEV1992815.1"
                     /translation="MGIHTFDELRAIVGQRGFISSPEELHTYECDGLTNFRVMPRAVL
                     LPNSTEQVQAIVRVCHRERIPFVARGSGTGLSGGALPVENGIVISLARMNRILEVDFP
                     NARVVVEPGVINLDVTGRVSPQDYFYAPDPSSQSICSIGGNVAENAGGAHCLKYGFTT
                     THVLGLEVVLPDGSLVHFGGKTLDTPGYDLAGVFVGSEGTLGVATKIILRIVKRPECI
                     RTLLAAFPSTNEAGAAVSDIIAAGMLPAAIEMMDNLAIQAAEAAVHPNYPSCGGLLLV
                     ELDGPAAEVEALMTDVNEICRSNGASEIRMAQSDEERALVWKGRKSAFAAMGRISPNY
                     IVQDGVIPRTALPRVMSEIERLSQEAGLRVANVFHAGDGNLHPLVLYDRRIPGQEEAA
                     EALSNNILELCIAAGGSITGEHGVGEDKKKMMSKMFAEPDLATMQRVRCAFDPLQLSN
                     PTKIFPTPRLCGEKPGEYVPHPLESAGIAERY"
     gene            4721..6013
                     /locus_tag="VGR03_00630"
     CDS             4721..6013
                     /locus_tag="VGR03_00630"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV1992816.1"
                     /translation="MDAATKSGASGLHSALRYKFIALVGNEYVRAATTADLVAGVQPK
                     LVIEPGTESELAEVLRLSNEAGLAVIPRGGGTKLGWGNPPARADLILSTVRMSEIIEH
                     AWADLTVTVEAGCTIQRLQETLAQHGQRLALDPLWPEKATIGGVLSTNDSGALRLRFG
                     ALRDLIIGATIALPDGTLASSGGKVVKNVAGYDLPKLVTGALGTLGVITRAVFRLHPL
                     PRNSRAFSVSGADAEEMQRFVLAVQNSKLAHTFLQSHFSDDAPPVSDILFEGTEAGLA
                     AQETQLRTLSAPANVSEASTSTWTAREELWAFSEPASTAIAKISILPANLARTIKLVE
                     HIANTQQIRWRAVTYATGLGWLRLEGKAGSLRGALQALRGELHGQDGSLVALHRPDKM
                     PAFDAWGRAGDALALMKAVKHQLDPKNTLNPGRFVGGI"
     gene            6013..7341
                     /gene="glcF"
                     /locus_tag="VGR03_00635"
     CDS             6013..7341
                     /gene="glcF"
                     /locus_tag="VGR03_00635"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: protein motif:HMM:NF008434.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEV1992817.1"
                     /translation="MEDLTAKGECASAFDAHHPPSNEIIEKCVHCGFCLPVCPTYVLW
                     GQEMDSPRGRIYLMKMAREGAAEMNSTWVSHFDSCLGCMACTTACPSGVDYGKLIEAT
                     RAQIERKTKRSPWEKLHRRFMFETFTRPDRLRRMRLPLLAYQKSGVQAVVRGLGLLKL
                     LPKKVQAMEALLPKLAPRETVAELTPAVGTKRRRVGLLLGCVQREFLSQVNAATVRVL
                     AAEGCEVVAPQAQPCCGALLVHAGEEAAAVELAKRTIDAFESADVETIVTNAAGCGSN
                     VKEYGHLLRDDPDYAGRAKSFAAKCKDISEILAELEPHAARHRLKLRVAFHDSCHLQH
                     AQGIRSQPRALLSSIPGLELAEIPEAAICCGSAGIFNLVQPDAANELGDRKAQLIAPL
                     RADVVATGNPGCLLQMQSALARQGQKIPVVHTIQLLDASIRGESSETFRR"
     gene            complement(7359..>8224)
                     /locus_tag="VGR03_00640"
     CDS             complement(7359..>8224)
                     /locus_tag="VGR03_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEV1992818.1"
                     /translation="RSTLSTATVDGDNGLGLVVGPEANRIAMDMAEKAGSGWVSVRNT
                     NHFGIAGYYVLKALERDLIGWAMTNSTSLVAPLWGAERMLGTNPISIAFPGKEELPIV
                     IDMATSAAAYGKIEIARRRGEPIPEGWAIDSEGRATTNPDDMVAGGALLPLGSDRERG
                     GHKGYALAIMVDVLCGALSGANWGPFTPPFALRQEIPKRSVGKGIGHFFGAMRIDGFM
                     DSDSFKRQIDDYIRVFRATKPAPGTNGPLIPGDPEREMEQLRREKGVPLILPVVEELR
                     DISQKIGIPFE"
BASE COUNT         1522 a         2489 c         2383 g         1830 t
ORIGIN      
        1 tgcgcgcggc cccggctatc ccgcattcct cgctgggatt tatttcctgg ccggtacggg
       61 ccgccaggcc gtcatgctcg cgcaagcctt cgtcgatctt gccacgtgcg tcttggctgc
      121 gggcatcgcc catcgtctgg ccgcaggcgc tcccgacgcg acgcgcaccc gcgttgccgc
      181 agcagcgctc tggctcaccg ctctttgtcc attcacagcg aactatgctg ccgtgccgct
      241 cacagaggtt ttggcaactt ttttcaccac gctcgcgctt ctgatttttc tgtcgcccgc
      301 ggcaacggag atcgaccgca ttgcctcaaa ccccgatctg cttcgcgcca caaaaatttg
      361 gttcgcaggc ggactcgtta ttggtctcgg cactctcgtc cggccggaaa cgccgctgct
      421 tcttggagcc gtgctgatcg cgctttggct tcgctatcgc cgtcccgcga attggaaaag
      481 actagcgctc gcaacgttgt ggatgatcgt tggtttgttg ctgccgctgg cgccctgggc
      541 cgcccgaaat tccatcagcg ttggacgtgc acaatttctc gcgcctcgct acgcggagac
      601 ctacggcgat gttctgccga ccggctttta cgcctggacg aaaacctgga tgtttcgttt
      661 tcgcgacgcc tatctattta cctggaaatt gccgatgcag ccaatcgact tgaaaaatct
      721 tccttcatac gcagtggatt ctcccgaaga attgtcacgc gtcacttccc tgctggatcg
      781 ctacaaccgc gcgcgcggca tgacccgctc actcgatctc gaattcgccg agttggcaca
      841 gaaacgcgcg aagcgtcatc cgattcgcac ctatgtgtgg attcccgtcg agcgcgccgc
      901 cgccatgtgg ttcactccgc gcattacctt gctgccttac tcaggcaggc tctggccgct
      961 caccgaaagt taccgcagca atcccaccga cttcgaagtt actctcggct tggcgcttct
     1021 aagtgttctg tatgttggaa taggtcttgc agccgcgtgg tcctcccgca cgaatcccgg
     1081 agtctcgctg atcgtggcct tcatccttat tcgcacggca ttcctcacac aattgcaaac
     1141 ctgcgaaccg cgctacgtct tggtctgctt ccccgctctg ctggccgtgg gcgcgcaact
     1201 ttggcggcac tcaaaaaccg gccgagaaat agttatcgct tgactagaac gcggtaagga
     1261 agtatctcag cgactgaaac ctaaggattc cgggataacg cggtggctca gccaggcagt
     1321 cttccttttc gcaggtggtt tgggatattg agctcgccgg aatttcccaa tactggtgtg
     1381 cgggagattc tgctttgact tggacacaag tttatgaccc cttgggacat tggtggctct
     1441 cgaccctcgt cgcgaccctt cctgtggttg ttcttctcgg agcgttggcg gtattgcgca
     1501 taaaggcgca ctgggccgcg ctactcgggc tggcctcttc tctgctcatc gccgtcggca
     1561 tattcaaaat gcccggagcg atggcggcgc gcacggctgt gtttggcgct ggttacggcc
     1621 ttttcccgat cggctggatt atcctcaacg tcatctttat gtacgacatg aactgcgcct
     1681 cgggccgctt caaagtgttg caagagagcc tgatgggtat cacccaagat cggcggctgc
     1741 agcttctgtt gatcgccttc tcttttggag cgttttttga aggcgcggcg gggtttggca
     1801 cgcctgttgc cgtcaccgcc gccatcctga ttggcttggg cttcaagccg ctgcaggcct
     1861 cgggcctttc gctcattgcc aacaccgcgc cggtggccta tggcgcgctc ggcacgccgc
     1921 tcgttgctct tcaggctgtc accggcctcg atcttttgca gctcagcgcc atggccggac
     1981 gaattctgcc attcttctct gttctggtgc ccttctggct cttgtgggcc tttgccggat
     2041 tcagcgggat gctggagatt tggcctgccg tgctcgttgc aggggtcttc ttcgcagttc
     2101 cacaatttct ggtgtccaat tttcatgggc cgtggcttgt ggacgtggtt tcggcggttt
     2161 gctccatggc agccgtgatc gtcttgctcc tcctctggca tcccaagaaa atctggggtt
     2221 ttgaaggaca cgaatccgat cgtagcgcgc gcggccagca cggtcacagt gcgggagcag
     2281 tcgccaaggc ctgggttccc tgggtcattc tcagcgtgtt ggtctttctc tggggtctcc
     2341 cgagcgtcaa gacccttctc gataagatct ctattttcaa aatcccagtc agcggtttgc
     2401 acaacctcat tctgagggtg cctccggtag tcgccaagag cacgcccgag gccgctgtgt
     2461 ttaatttcaa ctggctgtcc gccaccggta gcggcatcct gatagcggct gtcatttctg
     2521 gcttcgtgat gggtttcggt cccttgcaga tgctgcgcca atacgggaag accttgtaca
     2581 aagtgcgatt ctctttgatc accatagccg ccatgatgtc tatcggtttc atgacgcgct
     2641 attctggcct ggatgcgacc atgggattgg cctttgctcg cacaggcact ctttatcctt
     2701 ttttcggcac tatgctcgga tggctcggcg ttgcgctcac cggctcggat acctcctcaa
     2761 acgtgctgtt cggcagcttg cagaaaatct ccgcaaccca ggtcggtgtt tccccgacat
     2821 tgatggcggc ggccaatagc tgcggcggcg tgatgggcaa aatgatagat gcccagagca
     2881 tcgtcgtcgc cagcaccgcc acggaatggt acggccacga gggagacatc ctgcgttacg
     2941 tcttctttca cagcatcgcg ctggcttgcc tggtaggggt cctagtgttc ctggaagcct
     3001 acgtctttcc gttcaccaaa atggtcatac actgagtccg atggccctct aaatctacat
     3061 ttttcagcac tatacagttc ttggtaaggg cgcggtaccc gcttcggatg aagttggcgt
     3121 tcgtgctctc aattactatc tggattccat cggacgcaat gccgaactct cttaccttgt
     3181 tccaaggcac gagtctgtga acggagagga atctttcttc tttctgcgga agagccgtac
     3241 tattcttcat ctggacacaa agatgggcat tcacactttc gacgaacttc gagccatcgt
     3301 gggtcagcgt ggcttcatct cttcgcccga agaacttcac acctatgagt gcgacggcct
     3361 gaccaatttc cgcgtcatgc cccgcgcggt cctcctgcca aattccacgg aacaagttca
     3421 ggcgatcgtg cgcgtttgcc accgtgaacg aatccccttc gttgcgcgcg gctcgggcac
     3481 aggcttgagc ggcggtgcgc tccccgttga aaacggtatc gtcatcagcc tggcgcggat
     3541 gaatcgcatc cttgaggttg attttccaaa tgctcgcgtc gtcgtcgaac ccggggtcat
     3601 caatctcgat gtcaccggac gcgtctcacc gcaagattat ttttacgcgc ccgatccgtc
     3661 ctcacaatcg atttgcagca tcggcggaaa cgtcgcggaa aatgccggcg gggcgcattg
     3721 cctgaagtac ggttttacga ccacgcatgt cctgggcctg gaagtcgtcc ttccggacgg
     3781 ctcgctcgtc catttcggcg gaaaaactct cgacacgccc ggctacgacc tcgccggagt
     3841 tttcgtgggc tcggagggca ctctcggcgt tgccaccaaa attattttgc gcatcgtaaa
     3901 gcggcccgaa tgcattcgaa ctttgctcgc cgctttccct tccacaaacg aagctggcgc
     3961 ggcggtgagc gatatcatcg ccgccggcat gctccccgcc gccatcgaga tgatggacaa
     4021 tctcgctatc caagctgcgg aagccgccgt gcaccccaac tatccgagct gcggtggcct
     4081 gctcttggtt gagctagatg gccccgccgc ggaagtggaa gccttgatga ctgacgttaa
     4141 cgagatttgc cgttcgaatg gcgcgtcgga aattcgcatg gcgcagtcgg acgaggaacg
     4201 cgcgctcgtc tggaaaggcc gcaaatccgc ctttgcggcg atggggcgca tctctcccaa
     4261 ctacattgtc caggacggag tcattccgcg caccgctctt ccccgagtga tgagcgaaat
     4321 tgagcgcctc agccaagaag caggactgcg cgtcgccaat gtgtttcacg ccggcgacgg
     4381 caatctgcac ccgctggtcc tctacgaccg ccgaattccc ggtcaagagg aagccgccga
     4441 agctctctcg aacaatattc tcgaattgtg catcgcggca ggcggctcga tcaccggcga
     4501 acacggcgtt ggcgaagaca aaaagaaaat gatgtcgaaa atgtttgccg agcccgattt
     4561 ggcaaccatg cagcgagtcc gctgcgcttt cgatccgctg cagctttcga atcccacgaa
     4621 aatctttccg accccgcggc tctgcggcga aaaaccgggg gaatacgtgc cgcacccgct
     4681 tgaatccgcg ggaatcgcgg agcgctactg acgctggccg atggacgccg caaccaaatc
     4741 cggagcgagc ggtttgcatt cggcactgcg ttacaaattc atcgcccttg tcgggaatga
     4801 gtacgtgcgg gcggcgacta ctgcggactt agtcgccgga gtccagccga agctggtgat
     4861 tgaaccggga acggaaagcg agcttgccga ggttttgcgg ctttcgaatg aggcggggct
     4921 ggctgtgatt ccgcggggcg gggggacgaa gctcggctgg ggaaatccgc cggcgcgcgc
     4981 ggatttgatt ttgtcaacgg tgcggatgag cgaaatcatc gagcatgcct gggccgatct
     5041 caccgtcacc gtcgaagcgg gatgcactat tcaaagattg caggaaacac tcgcgcaaca
     5101 tggccagcgg ctggcgctcg atccgctgtg gcccgaaaag gccaccatcg gcggcgtgct
     5161 ctccaccaac gacagcggcg cgctccgttt gcgttttggc gcgctgcgcg acctgatcat
     5221 tggcgccacc attgcgctcc cggacggcac gctcgcgagc agcggcggca aagtcgtgaa
     5281 aaacgtggct ggttacgatt tgccgaagct cgtgaccggg gccctcggca ctctcggcgt
     5341 catcacccgc gccgtctttc gcctccatcc ccttccccgg aactcgcgcg ccttttccgt
     5401 ttctggtgct gacgccgaag agatgcagcg attcgtgctc gcagttcaga attcgaaact
     5461 cgctcacacc tttctccaat cccatttctc ggatgacgct ccgcctgtct ctgacattct
     5521 gtttgaaggc acggaggccg gcctagcagc gcaggagacg caactgcgaa ctttgtccgc
     5581 acctgcaaac gtttcggagg cttccacgtc aacctggacg gcgcgcgagg agctttgggc
     5641 tttttcggag cctgcaagca cggccatcgc gaaaatcagc atcttgccgg caaacttggc
     5701 tcgcacgatt aagctggtgg agcacattgc aaacacgcaa cagatccgat ggagagcggt
     5761 gacgtatgca acgggcctcg ggtggcttcg tttggaaggc aaggccggtt cgctgcgcgg
     5821 cgcgcttcag gcgttgcgcg gcgaattgca tggccaggat ggttcacttg tcgcgctcca
     5881 tcgtccggac aagatgccgg cattcgacgc ctgggggcgc gccggtgatg cgctcgcatt
     5941 gatgaaagcg gtgaagcacc aactcgatcc aaaaaacacg ctgaatcccg gccggtttgt
     6001 gggtggcatc tgatggaaga tttgaccgcc aagggggaat gcgcgagcgc ctttgacgcg
     6061 caccatccgc cttcgaacga gatcatcgaa aagtgcgttc actgcggatt ttgtttgccc
     6121 gtgtgtccca cgtacgtttt gtggggccag gaaatggatt cgccgcgcgg ccgcatctac
     6181 ctaatgaaaa tggcgaggga gggcgccgcg gaaatgaatt cgacgtgggt gagccacttc
     6241 gatagctgcc tcggctgcat ggcttgcacg accgcgtgcc cttccggcgt ggactatgga
     6301 aaactcatcg aggccacgcg cgcacaaatc gaacgaaaga ccaaacgttc accgtgggaa
     6361 aagctccacc ggcgatttat gttcgaaact tttacgcggc cggatcgctt gcggcgaatg
     6421 cgcttgcctc tgcttgcgta tcagaaatcc ggggtgcagg ccgtcgtgcg cggcttgggc
     6481 ctgctgaagt tgttgccgaa gaaggtgcag gcgatggaag cgcttcttcc gaagcttgcg
     6541 ccgcgtgaaa cggtcgcgga gctcacgccc gccgtgggca caaagcgccg ccgggttggt
     6601 ttgctgctgg gctgcgtgca gcgcgaattt ttatcgcaag tcaatgccgc cacggtgcgc
     6661 gtccttgccg ccgaaggctg cgaagtcgtt gcgccgcagg cgcagccgtg ttgcggagct
     6721 cttttggtcc acgcgggaga ggaagcagca gcggtggagc tggcgaaaag gacgatcgac
     6781 gcgttcgaaa gcgcggacgt cgagactatt gtcaccaacg cggcaggatg cggctcaaac
     6841 gtgaaagagt acgggcacct gctcagggat gatcccgatt atgccggtcg agccaagagc
     6901 ttcgctgcaa agtgcaaaga catcagcgaa atactggccg aacttgaacc acacgccgca
     6961 cgccatcggc tcaaactgcg agtggcgttc cacgattcgt gtcacttgca gcatgcgcaa
     7021 ggcattcgtt cacaaccccg cgctttactt tccagcattc ccgggctgga gcttgcagaa
     7081 attcccgaag ccgccatctg ctgcggctcc gcgggaatct tcaatctcgt gcagcccgat
     7141 gccgccaatg agcttgggga ccgtaaggcg caactcatcg ctccgttgag ggccgatgtg
     7201 gtggcgacgg gaaatcccgg atgtttgttg caaatgcaat ccgcactggc gcgccaaggc
     7261 cagaagattc ccgtcgtgca tacgattcaa ctcctggatg cttcgattcg cggcgagtcg
     7321 agcgaaacgt tccggcgata atccatccct gcaagctgct actcaaaggg aatgccgatt
     7381 ttttgggaga tgtcccgcaa ctcctcgacg accggcaaaa tcagcggcac acctttctcg
     7441 cggcgcaatt gttccatctc ccgttcggga tcgcccggaa tcagcggacc gttggtgcca
     7501 ggcgctggtt tggtggcgcg aaaaacacgg atgtagtcgt caatctgccg cttaaaagaa
     7561 tcactatcca tgaagccgtc gatgcgcatc gctccgaaga aatggccgat gcctttcccg
     7621 acgctgcgtt tcggaatctc ctggcgaagg gcaaagggcg gcgtaaacgg cccccaattc
     7681 gcgccgctga gagcaccgca caggacgtcc accatgatgg ccagtgcgta gcccttatgt
     7741 cctccgcgtt cgcgatccga acctagcggc aagagcgcgc ctccggcgac catgtcgtcc
     7801 ggattagtcg tggcgcggcc ctcgctgtcg attgcccagc cctcgggaat aggctcgccg
     7861 cgccggcgcg caatttcgat ttttccgtac gcggcggcgc tggtggccat atcaatcacg
     7921 atgggcagtt cttccttgcc cggaaaggcg atggaaatgg gattggtgcc gagcatgcgc
     7981 tccgcgcccc acaacggcgc gaccagactc gtcgaatttg tcatggccca gccgatcagg
     8041 tcgcgttcca gtgctttgag cacgtagtag ccggcgatgc cgaaatggtt ggtgttccgc
     8101 acgctgaccc agcccgagcc ggctttctcc gccatgtcca tggcgatgcg attcgcttcc
     8161 gggcccacaa ctaaacccag gccattgtcg ccatccacgg ttgcggtact cagcgtcgag
     8221 cgca
//
LOCUS       DASYVO010000015         5755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_197946, whole genome shotgun sequence.
ACCESSION   DASYVO010000015 DASYVO010000000
VERSION     DASYVO010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197946"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VGR03_00645"
     CDS             <1..221
                     /locus_tag="VGR03_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEV1992819.1"
                     /translation="AKVHMNCGEIVSMRGVTPATVSHHLKILSEAGLIECRREGQFVY
                     SQAVPETIEAYTRALTKIAAGKKSARRR"
     gene            complement(232..1863)
                     /locus_tag="VGR03_00650"
     CDS             complement(232..1863)
                     /locus_tag="VGR03_00650"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEV1992820.1"
                     /translation="MIEATHTLRKPVESRRALHQRKKTLALGIFLAFGIAGALAMLLI
                     GCGGGSSAPVAPPPVPAVQALQAADVQNIVQAAVNSVNVDMAVAVVDRAGFVLGVFRT
                     QNAPATAAGNFGQVQDANEVAVALARTGAFFSNDQAPLSSRTVRFISGIHFPAGVMNQ
                     PPADLYGIENTNRGCTLINDPIFQSKIPPSLALGGGFGLGVLTGKADTTDSNATAVNP
                     GGVPIFYKNVVVGGIGVVTMSSNLNVAEYAAFAGSTAARSGPTDTFGPTPAAPGVVFI
                     GGIALPFVSQTSRPTGFSAGPVAGTGIFAVAPTNSQGQPPEGDLIAPAAGPLGGLSAA
                     DVKQILDNAEATANTARAAIRLPLGSRTRMVIAVADLDGTIIGLRRMQDSTVFSIDVA
                     ASKARNMVYFNGASRTAADLNGVPTGTAVTNRTISFGAQPFYPPGIDGSSAGPFFSLY
                     MMDLANPCTQGFQSGAANANKSGIVFFPGSAGLYRSGALVGGLGVSGDGVDQDDYVTS
                     GGTKGFEAPASIRADQIMDQGVRLPYFKFPRNPTD"
     gene            2069..4312
                     /locus_tag="VGR03_00655"
     CDS             2069..4312
                     /locus_tag="VGR03_00655"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV1992821.1"
                     /translation="MILFSPRQVRRAFCAVCCFACLVFAVRAVATSPSQSPPAAQDSA
                     KQSREPGPPLRIPRQQAQTTAALDGVVRDAGFSNITLPVPAAVLALRNVQSGQIFSAT
                     TSGEGVFRVFPLPPGHYQLRVEAKDYAPFVLADLALQPNEVVTLEISLVTVAAMEARS
                     RLPRLPELGPALSAEAQPSFGTYREFRHRLDSDPNYIENISPDTLPPVADVFNTVPNR
                     WALEQPDYRRYSHKGEYVYSKPHWYDPFNHNRFKGDEPIWPELLGQQAFLNITASSES
                     FFDGRRVPSPSNVSSARPGSSGFFGQGEQAFFDQTLRFTLDLFHGDTSFKPVDWRIRI
                     TPELSLNNLKVREIGLVGPDPRSGTNRFDHHVGFQEAFVEVKLRDLGPNYDFISARAG
                     IQQFNADFRGFLFVDEEPALRIFENLHSGRIEYNVAYFHFLEKNTNSGLNTFDRRHQQ
                     VLLGNVYLQDFFFPGYTAEFVAAWNKDDPSLHYDDNGFLVRPAPIGSVINQNPGAGPI
                     LHGIRVGYFGWLGNGHIHRLNLTHAFYQAIGEDTFNPIAGRRVTVNAQMAAAEISYDK
                     DWIRYRISGFYTSGDAHPRDSRARGFDSIVDLPNFAGGLFSFWNREGIRLLGSGILLT
                     TPGSLIPSLRASKEEGQANFVNPGIFLANAGADFDITPKLRGFANFNFLRFERTEPLE
                     FFLFQSPIHHTIGEDFGVGVEYRPPLTENIVLTGGASALQPGQGFKDIYTSRTLFSLF
                     GSVKFTF"
     gene            4541..>5755
                     /locus_tag="VGR03_00660"
     CDS             4541..>5755
                     /locus_tag="VGR03_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992822.1"
                     /translation="MHPTKTVHLGCADCHGGNSSVSVASGAPPNSPEYNSAKEKAHVQ
                     PRDSSFKNRSALPERTYTKWLAESAEYIKFVNPGDLRVTPETCGTLGCHAAETRAVST
                     SMMTHAGMLWGVALYNNGGYPTKNTRFGESYDRDGKPQSIKTSPKPTLEETRAKGVIP
                     ELDPLFRWETSQPGNALRVFERGGRMKAELGNPNREEDPGKPDDKLGERGFGTGLRTD
                     PVFLGLQKTRLLDPVMSLPGTNDHPGDYRASGCTACHVIYANDRDPSHSGAYAQFGHS
                     GFSASSDPTIPKNESGHPIKHVFTRSIPSSQCMICHIHPGTNMVTTYFGLTWWDNEID
                     GDKMYPKEQRNPTEDQRYQSFMRNPEGAAVRGLWNDEKFLENVGSPEFNKQLKTTQFA
                     DFHGHGWVFRAVF"
BASE COUNT         1144 a         2007 c         1382 g         1222 t
ORIGIN      
        1 ccgccaaggt ccacatgaat tgcggcgaaa tcgtttcgat gcgtggcgta acccctgcaa
       61 ccgtttcaca ccatctcaag atattgagtg aagcaggcct catcgaatgc cgccgcgaag
      121 gacagtttgt ttacagccag gccgttcctg aaaccatcga agcgtacaca cgtgcgctca
      181 caaaaatcgc cgccggcaag aaatcggcac gccgtcgctg aatggaatcg tttagtcggt
      241 gggatttcta ggaaatttga aatacggcag ccgcacgccc tgatccatga tttgatccgc
      301 tcggatactc gccggcgctt cgaaaccctt ggtgccgccg ctggtgacgt agtcgtcttg
      361 atcgacgccg tcgccgctca cacccaatcc gccgactagc gccccgctgc gatacaagcc
      421 tgcactcccc ggaaagaaaa cgattccgct cttgttcgcg ttcgctgcgc cgctctgaaa
      481 tccctgcgtg cagggatttg ccaaatccat catatacaga ctgaaaaatg gcccggcact
      541 cgaaccatcg attcccggcg gatagaatgg ctgcgcgcca aaactgatcg tccggttcgt
      601 caccgccgtt cccgtcggca cgccgttcaa gtccgccgct gtccgcgaag caccgttgaa
      661 gtacaccatg ttccgcgcct tgctcgccgc cacgtcaatg ctgaacaccg tcgaatcctg
      721 catccggcgc aatccgataa tcgttccatc caggtccgcc accgcgatta ccatgcgagt
      781 gcgtgaaccc agcggcaatc gaattgccgc gcgcgccgta ttcgccgttg cttctgcgtt
      841 atccaaaatc tgcttcacat ccgcagcgct caagccgccc agcggtcccg cagccggcgc
      901 aatcaaatcg ccctctggcg gctgcccctg actgttcgtc ggcgccaccg caaaaattcc
      961 cgttccagcg acgggtcctg cagagaaacc cgtaggccgc gacgtctgac ttacgaacgg
     1021 cagcgcaatc ccaccaataa aaaccactcc gggagcagcc ggagtcggcc caaacgtatc
     1081 cgtgggcccg ctacgcgcgg ccgtcgatcc cgcgaatgcc gcgtactccg ctacattcag
     1141 attgctcgac attgtcacga ctccgattcc accgaccacc acattcttgt aaaaaatagg
     1201 cacgccgccc ggattcaccg ccgtcgcatt cgaatctgtg gtgtcggcct tgccggtaag
     1261 cactccaagc ccgaatccac cgcccaacgc cagggatgga gggattttgc tctggaatat
     1321 cgggtcgtta atcagcgtgc agccgcgatt ggtgttctcg atcccgtaca aatcggctgg
     1381 cggctggttc atcacacccg ccggaaaatg aatcccgctg ataaacctca cggttcgcga
     1441 cgagagcggc gcctggtcgt tactgaaaaa cgcgcctgtc cgcgccagcg ctacagccac
     1501 ttcgttggcg tcttgcactt gtccaaaatt tccggccgcc gtggccggcg cattctgcgt
     1561 tcgaaacaca cccagtacga atcccgcgcg gtccaccacc gccacggcca tatccacatt
     1621 cacggaattc accgcggcct gcacgatatt ctggacatcg gctgcttgca gagcttgcac
     1681 cgccggaacc ggtggcggcg caaccggtgc gctgcttccg ccaccacacc cgatcagcag
     1741 catcgcgagc gctccggcga ttccgaacgc caggaaaatt cccagcgcca gcgttttctt
     1801 tcgttggtgc agtgcgcgcc gcgattccac tggcttccgc agtgtatgag ttgcttcaat
     1861 cacgagagta cctgcgttcg tatcacactc gtttgctgcc actcaacgcc tttgccggtc
     1921 cttgccgcga actggtgaac gtatcgcgcc tttccttgcg ttatcgaatg actccacgcc
     1981 tccgggaaaa gtgttgctcg ccgctcgccg atattgttat ctagtacgcg attcctgttt
     2041 ctttcatcga tccgttcagg ccgaacgcat gatccttttc tctccgcggc aggtacgacg
     2101 cgcgttctgc gcggtttgct gctttgcctg cctcgttttt gcggtgcgcg ccgtcgctac
     2161 ttctccatct cagtctcctc ccgcggcgca agactccgca aaacaatctc gcgaacccgg
     2221 cccgccgctg cgcatccccc gccagcaggc gcagaccacc gccgcgctcg atggcgttgt
     2281 ccgcgacgcc ggcttctcca acatcactct tccggttccc gccgcagttc tcgcgttgcg
     2341 caacgtccaa tccggccaaa tcttcagcgc taccacttcg ggagaaggcg tcttccgggt
     2401 tttcccgttg ccgccgggcc attaccagct ccgcgtcgaa gccaaggatt acgcgccgtt
     2461 cgttcttgcc gatcttgctc ttcagccaaa cgaagttgtg accctggaaa tctctctcgt
     2521 cactgtcgca gccatggagg cgcgttcgcg cctcccacgc cttcccgagc ttggcccagc
     2581 tctctccgct gaagcgcaac catcgttcgg aacttatcga gagtttcgcc atcgtctcga
     2641 ttccgatccc aactacatcg aaaatatttc gccggatacg cttcctccgg tcgcggacgt
     2701 tttcaatacc gttccaaacc gctgggctct cgaacagccc gactaccgcc gctattcgca
     2761 taaaggcgag tacgtctact cgaagcctca ctggtatgat cctttcaacc acaatcgctt
     2821 caaaggcgac gagcccatct ggccagagct tctcggccag caagcttttc tgaacatcac
     2881 cgcttcttcc gaatcatttt tcgatggccg tcgcgtcccc tcgcccagca acgtcagtag
     2941 cgcgcgcccg ggcagctccg gtttctttgg ccaaggggaa caagcctttt tcgatcaaac
     3001 gcttcgtttc acccttgatc tatttcacgg tgacacttct tttaagcccg tcgactggcg
     3061 cattcgcatc actccggagc ttagcctcaa caatttgaag gttcgcgaga tcggcctcgt
     3121 cggccccgat ccccgcagcg gcaccaaccg tttcgaccat catgtcggtt ttcaggaagc
     3181 cttcgtcgaa gtgaagctcc gcgacctcgg ccccaattat gatttcattt ccgcgcgtgc
     3241 tggcattcag caattcaacg ccgacttccg cggctttctt tttgtcgacg aagagccggc
     3301 tctgcgcatc ttcgaaaatc ttcatagcgg tcgcatcgag tacaacgtcg cctacttcca
     3361 tttccttgaa aaaaacacca acagcggact caacacgttc gaccgccgcc accagcaggt
     3421 ccttctcggc aacgtctatc tccaggactt cttttttccc ggctacaccg cggagttcgt
     3481 cgccgcctgg aacaaggacg atcccagcct ccactatgac gacaacggct tcctcgttcg
     3541 ccctgcgccc atcggcagtg tcatcaacca gaatcccggc gccggcccga ttcttcatgg
     3601 aattcgcgta ggctatttcg gctggctcgg caacggccac atccaccggc tcaatctcac
     3661 gcacgccttc tatcaagcca tcggtgagga caccttcaat cccatcgccg gacgccgcgt
     3721 caccgtcaac gcgcagatgg ccgccgcgga gatctcctat gacaaggact ggatccgcta
     3781 ccgcatctcc ggcttctaca cctccggcga cgctcatcct cgcgattccc gcgctcgcgg
     3841 tttcgattcc atcgtcgatc ttcccaattt tgccggcggt ctctttagtt tctggaatcg
     3901 cgaaggcatt cgccttctcg gctccggcat tctcctcacc acgccgggca gcctcattcc
     3961 ttctctccgc gccagcaaag aagaaggtca ggccaatttc gtcaaccccg gcatcttcct
     4021 ggccaacgcc ggcgccgact tcgatatcac tcccaagctg agaggcttcg ccaatttcaa
     4081 ctttctgcgt ttcgaacgca ccgagccgct cgaattcttt ctattccaat cgcccattca
     4141 ccacaccatc ggcgaagact tcggcgtagg cgttgaatat cgccccccgc tgactgaaaa
     4201 catcgttctg acgggcggcg cttccgcgct ccaacccggc cagggtttca aggatatcta
     4261 tacaagtcgc acgctcttct cgcttttcgg ctctgtgaag tttactttct gagatgcgcc
     4321 gatcatgaac caagaacgcc aacccgctcc ttcctctctg cgaaccctct gtgttctctg
     4381 cgtctctgcg ttatcttttt tctttgctct tcatgcaagc tcaaagcctc ccgcacgcgc
     4441 cccgcaagag tcagctccgt tccgcggcca atcgcaggaa gaagccgatc gcaaatcctc
     4501 gggctgcatc tcctgccaca cctcgaccga cgaacccacg atgcacccca cgaaaaccgt
     4561 ccatctcggc tgcgcggatt gccatggagg caactcctcc gtctcggtcg catccggggc
     4621 cccgccgaat tctcccgaat acaattccgc caaggaaaaa gcgcatgtcc agccgcgcga
     4681 ttcttcgttc aagaatcgct ccgcgcttcc cgaacgcacg tacaccaaat ggcttgcgga
     4741 atccgccgag tacatcaagt tcgtcaatcc cggcgacctc cgcgtcactc ccgaaacttg
     4801 cggaacgctc ggctgccatg ccgccgaaac tcgcgccgtt tccaccagca tgatgaccca
     4861 cgccggaatg ctctggggcg tagcgctgta caacaacggc ggctatccca caaaaaacac
     4921 gcgtttcggc gaaagctacg atcgtgacgg caagcctcaa tcgattaaga cttctcccaa
     4981 gcccacgctg gaagaaaccc gcgccaaggg cgtgattccc gagctcgatc ctctcttccg
     5041 ctgggagact tcgcagcccg gcaacgcgct tcgcgtgttc gaacgcggcg gccgcatgaa
     5101 ggcagaactc ggcaatccca atcgcgaaga ggaccccggc aagcctgacg acaagctcgg
     5161 cgaacgcggc ttcggcaccg gtcttcgcac cgatcctgtt ttcctcggcc tgcagaaaac
     5221 gcgcttgctc gaccccgtca tgtcgcttcc gggtaccaac gatcatcccg gtgactatcg
     5281 cgctagcggc tgcaccgcgt gtcacgtcat ctatgccaac gaccgcgacc catcacactc
     5341 cggcgcctac gcgcaattcg gccactccgg cttcagcgct tcttccgatc cgaccattcc
     5401 caaaaatgaa tccggccatc ccatcaaaca cgtcttcacg cgctccatcc cctccagcca
     5461 atgcatgatc tgccacatcc atcccggcac gaacatggtc actacctatt tcggcctcac
     5521 ctggtgggac aacgaaatcg acggcgacaa gatgtacccg aaggagcagc gcaatcccac
     5581 cgaagaccag cgctaccaat ccttcatgcg caatccggaa ggtgctgccg ttcgcggcct
     5641 ctggaacgac gaaaaatttc tggaaaacgt cggcagcccc gaatttaaca agcagttgaa
     5701 aaccacgcag ttcgccgatt tccacggtca cggctgggtt ttccgtgctg tcttc
//
LOCUS       DASYVO010000016         2693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_113086, whole genome shotgun sequence.
ACCESSION   DASYVO010000016 DASYVO010000000
VERSION     DASYVO010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113086"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="VGR03_00665"
     CDS             complement(<1..1185)
                     /locus_tag="VGR03_00665"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEV1992823.1"
                     /translation="MGPGLITGAADDDPSGISTYSVAGAAYGYATLWIALLTFPLMAA
                     IQLMCARLGMVTGRGLAAAVRIYYPRWVLWGACSILVVANIINICADLGGMAEATQLI
                     TGIRSLIWIPFYALFIIGLLFWTSYKLIAKIFKWLTLVLFAYVLASFYAHVDWRHALA
                     VTFVPHLEWSRGFLAVLVAILGTTISPYLFFWQAAEEVEEEQAKGRTLAQSKSATTGE
                     LRTARADTIAGMFFSNFIMYFIILTTAATLHAHGQTDITTARQAAEALRPLAGDGAYL
                     LFTLGLIGTGMLGVPVLVGSCAYAVAEGAAWRGSMADKPRSASKFYAVMAVAMALGLA
                     LNFAGFNAVKMLFWSAVINGLLAPPLILLVVLLTSSHKVMGKRVNSPLLRCLGWATFG
                     VMT"
     gene            complement(1529..1604)
                     /locus_tag="VGR03_00670"
     tRNA            complement(1529..1604)
                     /locus_tag="VGR03_00670"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1569..1571),aa:Thr,seq:cgt)
     gene            2045..2275
                     /gene="vapB"
                     /locus_tag="VGR03_00675"
     CDS             2045..2275
                     /gene="vapB"
                     /locus_tag="VGR03_00675"
                     /inference="COORDINATES: protein motif:HMM:NF040493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEV1992824.1"
                     /translation="MATAKVFKSGNSQAVRLPKAFRVKSKELEISRRGDEIVLRERRG
                     TMARAFDLLAALPADLTLAGRTQDKPQKRKRL"
     gene            2275..2682
                     /locus_tag="VGR03_00680"
     CDS             2275..2682
                     /locus_tag="VGR03_00680"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEV1992825.1"
                     /translation="MGARFLLDTNICIYIGRKKPEEVLRRFRTLKQGEAALSVITFGE
                     LVYGAEKSAQRAAALELLRELAEVLPVTGLPETAAEAYGTMRAELERKGQMIGNNDLW
                     IAAHAKAAGLTLVTNNEREFRRVRGLKVQNWAA"
BASE COUNT          611 a          709 c          867 g          506 t
ORIGIN      
        1 ggtcatgacg ccaaatgtgg cccagcctaa acacctgagt aacggcgaat tcacgcgctt
       61 gcccattact ttgtgactgc tggtcaggag gacgaccagc aggatcaggg gcggtgccag
      121 gaggccattg attactgccg accagaacag catttttacc gcgttaaaac cggcgaaatt
      181 cagcgcgagg cccaatgcca tggccacggc catcaccgcg tagaacttgc tcgcacttct
      241 tggtttgtcg gccatcgagc cacgccacgc tgctccttcc gccaccgcat aagcgcacga
      301 tcccaccagg accgggacgc ccaacatccc ggtgccgatc agacccaacg tgaagagcag
      361 gtacgcccca tcgccggcaa gtggacgaag ggcctcggcg gcctgccggg cggttgtgat
      421 gtcggtctgc ccatgtgcat gtaaagtggc ggcggtggtc agaatgatga aatacatgat
      481 gaaattcgaa aaaaacatac cggcgatggt gtcggcgcgc gcagttctta actccccagt
      541 ggtcgcgctc ttgctctggg cgagggttcg gcccttggcc tgttcttcct ccacctcctc
      601 ggcggcctgc caaaagaaga gataaggaga aatcgtggtg cccagaattg caaccagcac
      661 cgccaggaat ccgcgtgacc actccagatg gggcacgaac gtcaccgcca gcgcgtgcct
      721 ccaatcgacg tgggcgtaaa acgatgccaa cacgtaggcg aacaagacga gcgtcagcca
      781 cttgaaaatc ttcgcgatca gcttgtagga agtccaaaac aacagcccaa tgatgaagag
      841 cgcgtagaat ggaatccaga tcagggatcg aattcccgtg atgagttgcg tggcctccgc
      901 cataccaccg aggtcagcac agatgttgat gatgttagcg acgacgagaa tcgagcaggc
      961 tccccagagc acccagcgtg gatagtaaat gcgcacggcg gcggccagcc cgcgccccgt
     1021 aaccatgccc agccgtgcgc acatcagctg gatcgcggcc atcagcggaa acgtgagcag
     1081 cgcgatccac agcgtggcgt atccgtaggc tgctccggcg acggaatagg ttgagattcc
     1141 tgacggatca tcgtcggccg ccccagtgat cagtcccggt cccaagacgg cgaaggatct
     1201 cctcagaagg ttgggtcctt tgccggcttc gacattattg gcaggcggat tttttctcgc
     1261 agtcatttgg gtcggaagcg atgctctgcc gggtgccgca ccctttgcag tttttcaaag
     1321 gatgcgggtt ttacttttct gcgtggatcg gatccgcgcg cagcgcccgc gttacgggaa
     1381 tcaggtggag ttcagcgcca cccgcatggg tgaccttata ttgaggcgca aacaaaaagc
     1441 aaacaactcc agaataaaac acgaagggac agttgaaccg aatctgtacc agtcaccagt
     1501 ggcacttgac cttagatgac cgtaggattg gggtgcctgg agggggtcgg accctcaacc
     1561 acccgattac ggatcggggg ctcttgccat tgagctacag gcaccactgt tgatttgggt
     1621 tgcgccggtg ttaggttcgc gagaacttgc accggcattt ccatttctgc ggcattgtac
     1681 tggggtacgt gaacttgatg taactggaca gtgggcggaa aagaggccag tgtcgaggtc
     1741 tggacatcta ctgccctcga aggcaataaa atgggcagtg cttcggtcca acccgcttat
     1801 aatcaatact ttccgtactg gaggctgctt ggatttgcgc agaagtacag gtcagagtac
     1861 ggactaaggt catagtctac atcgtccctg aagacaggtt catcgcgcaa gaacaacgcg
     1921 atggagaaga gagcctcgct tcgcgcggag taacaggcca aaggtgacgg gcaggaagcc
     1981 tcctatcggg cggagcgcaa aatgtgtata ctgtatgtat atacattggg ggggagggtt
     2041 gcagatggcg acggccaagg tattcaaatc ggggaacagc caggcggtgc ggttgccgaa
     2101 ggcgttccgg gtaaagagca aggaattgga gatctcgcgg cgaggggatg agatcgtgct
     2161 gcgggaacgc cgcggcacga tggcgcgagc gtttgatttg ctggcggcgt tgcccgcgga
     2221 cttgacgctg gcaggacgga cacaggataa gccgcagaaa cgcaagaggc tttgatgggg
     2281 gcgcgcttcc tgctggacac gaacatctgc atttacatcg ggcgaaaaaa accggaggag
     2341 gtgctccggc ggtttcgcac gctgaagcag ggagaggcgg cgctctccgt gattacgttt
     2401 ggcgaattgg tgtacggggc ggagaaaagc gcgcagcgcg cggcagcgct cgaactgttg
     2461 cgggagctgg cggaggtgct tccggtgacg gggttgccgg agacggcggc ggaagcttat
     2521 ggaacgatgc gggcggagct ggaacgcaag gggcagatga tcgggaataa cgatctgtgg
     2581 attgcggcgc acgccaaagc ggcggggttg acgctggtca ccaataacga gcgggagttc
     2641 cggcgcgtgc gtggtttgaa ggtgcagaat tgggcggcgt agaggggaac tgc
//
LOCUS       DASYVO010000017         9781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_84810, whole genome shotgun sequence.
ACCESSION   DASYVO010000017 DASYVO010000000
VERSION     DASYVO010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9781
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84810"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VGR03_00685"
     CDS             complement(<1..1115)
                     /locus_tag="VGR03_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEV1992826.1"
                     /translation="MGRIVGIDLGTTNSLVAYADPQTGQPKCIPGPYGETLCPSVVSL
                     DPDGSIIVGEAAVRRLLTQPERTIYSVKRLMGRGPADVQSELRLFPFRIDPSSKSVIR
                     VMLGDRVFTPPEISAFILRELKNWAEAHFGEPVDRAVITVPAYFNDAQRQATKDAGKI
                     AGLEVLRLVNEPTAAALAYGLHEKQRGKVAVYDLGGGTFDISVLKLISTAEGDIFQVL
                     STNGDTHLGGDDIDNLLQAFVHEQILQHDQIDFSPHGELAQELRKALIDVKHQLSESE
                     VATLRFSLPNGKVFTHEFTRGALDALIRPVVDRTMVPVKQALADAQLKPTEMDEVVLV
                     GGTTRTPLIRRTVQEFFDRKPHIELNPDEVVALGAAV"
     gene            complement(1145..1825)
                     /gene="hscB"
                     /locus_tag="VGR03_00690"
     CDS             complement(1145..1825)
                     /gene="hscB"
                     /locus_tag="VGR03_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEV1992827.1"
                     /translation="MTSPSTATNPPLVCWNCHERTLGTHFCSGCGKLLQLPQAVDYFA
                     LFEMPRKLWIEMNGLEQKFLQLSWKLHPDNFVNATETERELSLKRSSELNDAYRTLRD
                     PVGRVEYLLGIEGARKEGEHKQQAPPELLEEVFELNESLDELREAKASGSDLARLKAR
                     LESAGKSFQAKLGEVDAQLQSTAREWDAALQGDAAARKKIMAQLNELLNRRSYIRNLV
                     TNVQKELL"
     gene            complement(1822..1944)
                     /locus_tag="VGR03_00695"
     CDS             complement(1822..1944)
                     /locus_tag="VGR03_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992828.1"
                     /translation="MLDLLGLFLDLLPDFVFEFVLGRLFLGFQEIWNWTVNKIL"
     gene            complement(2057..2419)
                     /locus_tag="VGR03_00700"
     CDS             complement(2057..2419)
                     /locus_tag="VGR03_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEV1992829.1"
                     /translation="MATEVASPNIAKIHLTERAARKIRELLEKDGVSPEQGGLRVGVQ
                     GGGCSGLSYAMRLDTQSRDRDKIFEEHGARLFVDQKSFLFLNGTTLEYEETLMRQGFV
                     FQNPNAARNCGCGSSFTA"
     gene            complement(2524..2940)
                     /gene="iscU"
                     /locus_tag="VGR03_00705"
     CDS             complement(2524..2940)
                     /gene="iscU"
                     /locus_tag="VGR03_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEV1992830.1"
                     /translation="MAYSDKVIEHYNHPRNVGSLSKEDPNVGTGLVGAPECGDVMKLQ
                     MKINPETQVIEEAKFKTFGCGSAIASSSLATEWVKGKTVEEALSIKNTDIVRELSLPP
                     VKIHCSVLAEDAIKAAIGDWKKKHGVEVPASEKPSH"
     gene            complement(2940..3251)
                     /locus_tag="VGR03_00710"
     CDS             complement(2940..3251)
                     /locus_tag="VGR03_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992831.1"
                     /translation="MANEGTIRIHRSIGGTGAAFRVTFVPYGGEGEEAAVAGERSFHE
                     LQQVRAFLKMLGLGAEFIKDALRQLTAGRSASVPNVSLTDKAVKSAGFGNLVNLARSN
                     G"
     gene            complement(3270..4490)
                     /locus_tag="VGR03_00715"
     CDS             complement(3270..4490)
                     /locus_tag="VGR03_00715"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF010611.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEV1992832.1"
                     /translation="MAVKLPIYMDNHATTPVDPRVVEAMLPYFTEKFGNAASRNHSFG
                     WAGEEAVETSRQLIASLINATPKEVIFTSGATESDNLMIKGVAEMFREKGNHIITQAI
                     EHKAVLDTCKNLEKHGFEVTYLPVQRDGRVSLDGLKAAIKPTTILITVMYANNEIGVI
                     NPIAEIGKIAKEHGIFFAVDGVQAVGKIPVDVQKDNIDLLAISGHKLYGPKGVGALYV
                     RRRNPRVQLSAIIDGGGHERGMRSGTLNVPGIVGLGKACEIAQKEMPEEAARMLALRE
                     RLRKGLEAKLDEVFINGSMEHRLPNNLNMSFAYVEGESLLMGINDIAVSSGSACTSAT
                     LEPSYVLKALGVGEDLAHTSIRFGLGRFNTQEEVDYVIDKMVQVVTKLRELSPLYEMA
                     KEGIDISKMKWQTA"
     gene            complement(4504..4941)
                     /locus_tag="VGR03_00720"
     CDS             complement(4504..4941)
                     /locus_tag="VGR03_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HEV1992833.1"
                     /translation="MFKLSKKADYGLIAVKHLAMHRQQHACSANEIAEQYGISATLMA
                     KVLQKLAHHSLVAAKHGSSGGYQLAKEPGQISALDVISAIDGPVLITSCVTSHGNCDA
                     TERCTVREPLRRVNESILNVLSTVTISQMSEEPHEPALVALRT"
     gene            5254..6231
                     /locus_tag="VGR03_00725"
     CDS             5254..6231
                     /locus_tag="VGR03_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1992834.1"
                     /translation="MAGRSVCGLILVVLLVPTHARAQTQSPSPTDRPKHAQVKASKKR
                     LSDRAFVLVGAGDIASCQDPEGARATAKLIEQIPGTVFAAGDLAYEKGSAEEFKNCYH
                     PAWGRFKDRTKPALGNHEYVEPTASGYFRYWGAQAGPQGKGYYSYDLGDWHIVVMNTN
                     CDAKNLGGCGGGSPQETWLKEDIAKHPNACILAYGHHALFSSGIFKNHAVHPELKQLW
                     QDLYAAHADLVLAGHEHSYERFAPQEPEGNADPAHGIREIVAGTGGRSLDLLGFATPN
                     SEIREWDTYGVLKLTLSPGKYTWGFIPEEGKSFRDSGSGVCHNQPTEAK"
     gene            complement(6260..8350)
                     /locus_tag="VGR03_00730"
     CDS             complement(6260..8350)
                     /locus_tag="VGR03_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEV1992835.1"
                     /translation="MAQNKRLQGLGILVLCVLLYPAMTAAQQPTLNLMPLPASAQPGT
                     GSLGVDSSFSVAFTGYTDPRLERAGERFLRQLARQTGLPLSLKPAKASKATLVIQTDH
                     ASKETQEVGEDESYILEVTTTGAKLNAPTPLGAMHGLQTFLQLVDVSPGGFAAPALKI
                     QDQPRFPWRGLMIDSARHFTPLDVIRRNIDGMEAVKMNVFHWHLSENQGFRAESRKFP
                     KLHELGSDALYYTQDEIRDLIAYARDRGIRVVPEFDMPGHSTAWFVGLPELASGKGPY
                     EIERKWGIFDPAMDPTNEKVYKFLDDLIGEMAKVFPDHYFHIGGDEVNGKEWDANPKI
                     QSFMKAHGIKNNEALQAYFSERVQKLVTKHGKAVVGWDEVLVAGVPKDIVIQSWRGQA
                     SLAQAAKQGYRGILSSGYYLDLGWSAARHYAVDPMSGDAANLSAEEKQRVLGGESCMW
                     AEYVSPENVDSRIWPRNAAIAERLWSPQEVRDPASMYARLDFVSARLEWLGLTHRTAS
                     RHMLQRLAGSASATEFAALRTLTDVIEPVKDYTREQTAPAEPTSATPMNRVVDAVPLE
                     SDAGRHFGDLVDQFLASACHDRAAESRLRAQLTAWRDNDAALQPLAQRSFLVQEVAGR
                     SQDLSTLALAGLAALDAIAKGQPAPDSWKAQQLAILEQVKKPKAQLLLIPAPAVQKLI
                     EAPAAGGACSSPKP"
     gene            8563..9045
                     /gene="coaD"
                     /locus_tag="VGR03_00735"
     CDS             8563..9045
                     /gene="coaD"
                     /locus_tag="VGR03_00735"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV1992836.1"
                     /translation="MKTSIAIYPGSFDPVTNGHLDLIERGEKMFDLLIVAVLRNTEKQ
                     PLFTVTERMEMLREVTGKWAAVEVDVFEGLLINYARKRGAGVILRGIRAVSDYEYELQ
                     MALMNRKLEPRLETVFMLPGETYSYLSSKLVREIAQHGGPLDGLVPPTVEQRLRAKVL
                     "
     gene            9123..>9781
                     /locus_tag="VGR03_00740"
     CDS             9123..>9781
                     /locus_tag="VGR03_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV1992837.1"
                     /translation="MQLTDRINRIQISATAAVIAEADKLKSRGVDIADFGPGEPDFPT
                     PDHIKMAAIRAIEENKSKYTPTGGIMPLREAICAWHKRELGSSYEPKECAVSVGGKHS
                     IFNVMSVLIQQGDEVIIPSPYWVSFPDIVKYAGGTPVFVETKPEDGFAVRDSAVAKAI
                     TPRTKLLILNSPSNPTGGVVPPDEYERILALCKKHNVWLLGDECYSHFTYEPHKPYSI
                     A"
BASE COUNT         1986 a         2865 c         2862 g         2068 t
ORIGIN      
        1 tgcacggcgg ccccgagcgc cacgacttcg tcgggattca gctcgatgtg cggtttgcga
       61 tcgaaaaact cctgcacggt gcggcgaatc agcggcgtgc gcgtggtgcc gccgacgagc
      121 acgacttcgt ccatttctgt gggcttgagc tgcgcgtccg caagcgcttg cttgacgggc
      181 accatggtgc ggtccaccac ggggcgaata agggcatcga gcgcaccgcg cgtaaattcg
      241 tgcgtgaaaa ctttgccgtt tggcaacgag aagcgaagcg tggcgacctc tgattcggaa
      301 agctgatgct tcacgtcgat cagtgctttg cgcagttcct gggcgagttc gccatgcggc
      361 gaaaaatcga tctggtcgtg ctggagaatc tgttcgtgga cgaaagcctg cagcagatta
      421 tcgatgtcgt cgccgcccag atgcgtgtcg ccgttggtgg aaagaacctg aaagatatcg
      481 ccttcggccg tggaaatcag tttgaggacg gaaatgtcaa acgtgccgcc gccgagatcg
      541 tagacggcaa ctttgccgcg ctgcttctcg tgcagaccgt aggcgagcgc cgccgcagtg
      601 ggctcgttga cgagacggag aacttcgagg ccggcgattt taccggcgtc cttggtggcc
      661 tggcgctggg catcattgaa ataagccggc acggtgatga cggcgcgatc gacgggttcg
      721 ccgaagtggg cttcagccca atttttcagc tcgcgcagta tgaaagcgga aatctccggc
      781 ggcgtgaaaa ctctatcgcc gagcataacg cggatgacac ttttgctcga tggatcgatg
      841 cggaatggga agagcctgag ttcgctttga acgtcggcag gtcctcggcc catcagccgc
      901 ttcacggaat aaatcgtgcg ctctggttgc gtgagcaagc ggcgaacggc ggcttcgcca
      961 acgatgatgc ttccgtcggg atcgaggctg acgactgagg ggcaaagcgt ctctccgtac
     1021 ggacccggga tacacttcgg ctgacccgtc tgaggatccg cgtaggcgac gaggctgttt
     1081 gttgttccga gatcgattcc tacgatgcgt cccatgatat gccgccaggg ctaaagcccc
     1141 tgcgctacaa aagctccttt tgaacgttgg tcaccagatt ccgaatgtag gagcggcgat
     1201 tcagcaattc attgagctgc gccatgatct tttttcgcgc agcggcgtcg ccttgcaggg
     1261 cagcatccca ttcgcgcgcg gttgattgaa gctgcgcgtc tacttcgccg agttttgcct
     1321 gaaaactttt tccggcggat tcgaggcgcg ctttcaatct ggcaaggtcg cttcccgaag
     1381 cttttgcttc gcgaagttcg tcgagcgact cgttcagctc gaagacttct tcgagcagct
     1441 ccggcggcgc ttgctgcttg tgctcgcctt ccttgcgcgc gccttcgatg cccagaagat
     1501 actcgacgcg gcccacggga tctcgcaagg ttcggtaggc gtcgttcaat tcggagctgc
     1561 gcttgaggga gagctcgcgc tccgtctcgg tggcgttcac aaaattgtcg ggatgcagct
     1621 tccaactaag ctgcagaaat ttctgctcca gcccattcat ttcgatccag agcttccgcg
     1681 gcatttcaaa gagggcgaaa tagtcgaccg cctggggcaa ctgcaagagc ttgccgcagc
     1741 ccgaacagaa gtgcgtgccg agggtgcgct cgtggcagtt ccagcagacc aggggcggat
     1801 tcgttgctgt cgagggcgag gtcataagat tttatttacg gtccagttcc agatctcctg
     1861 gaatcccaga aacagccggc caagaacgaa ctcaaacaca aaatccggca ggagatcgag
     1921 aaacaacccg agaagatcca gcatgtttta ctccgacgca ggcacaaaaa aagtggctgg
     1981 cgagtgcggc tgccaaccac ttgtgcggat cagcctgcgc cgcgtttgac cggggaacag
     2041 gctgaagcct gttctactag gccgtaaaag agctgccgca gccgcagttg cgcgccgcgt
     2101 tgggattctg gaaaacgaat ccctggcgca tcagcgtctc ttcgtactcg agcgtcgtgc
     2161 cgttcagaaa cagaaaactt ttctggtcaa cgaagaggcg cgcgccatgt tcttcgaaga
     2221 tcttgtcgcg atcccgcgac tgcgtgtcga ggcgcatggc ataagagaga ccggaacagc
     2281 cgccgccttg cacgccaacg cgcaggcctc cctgctcagg cgaaactcca tccttctcga
     2341 gcagctcgcg aattttccgc gccgcacgct cggtcaggtg gattttcgca atattcggac
     2401 ttgcgacttc tgttgccatc ttcaaatgct ccaaatccct tccgatttcg atggtgcgaa
     2461 gtaggggcgc agcatgctgc gcccctacaa ctcaggcgtc agcaatctag gccggccgca
     2521 ggcttagtgc gacggcttct cggaagccgg cacttcgacg ccgtgctttt tcttccagtc
     2581 gccgatggcg gccttgatgg cgtcttcggc aagcacggag caatggatct tcaccggcgg
     2641 gagcgaaagc tcgcgcacga tgtccgtgtt cttgatcgaa agcgcctctt cgaccgtctt
     2701 gcccttcacc cactcggtag cgagcgagga gctggcgata gccgagccgc agccgaaggt
     2761 cttgaacttg gcttcttcaa tgacctgcgt ctcgggattg atcttcatct gcagtttcat
     2821 gacgtcgccg cactccggcg ctccgacgag acccgtgccg acgttggggt cttccttcga
     2881 gaggctgccg acgttgcggg gatggttgta gtgctcaata actttgtcgc tgtatgccat
     2941 tagccgttgc tccttgccag attcacgaga ttcccgaaac ccgcgctctt gactgctttg
     3001 tccgtgagcg aaacgttcgg cacggaagcc gagcggcccg cggtcaactg acgaagagcg
     3061 tccttgatga attccgcgcc gagcccgagc attttcagaa aggcgcgcac ttgctgaagc
     3121 tcatggaaag agcgctcgcc ggcgacggcg gcctcttctc cttctccgcc gtagggcaca
     3181 aacgtcacgc ggaaagccgc gccggtgccg ccgatggagc gatgaatgcg aattgttcct
     3241 tcatttgcca tgttgtccgt tccagtcctt taggctgtct gccatttcat tttagagata
     3301 tcgattccct ctttggccat ttcgtagagc ggggacagtt cgcgcaactt ggttacgact
     3361 tgcaccatct tgtcgatgac gtagtcgact tcctcctgcg tattgaaccg gccgaggcca
     3421 aatcgaatcg aggtgtgggc gaggtcctcg ccgacgccta atgctttgag tacgtagctg
     3481 ggctcaaggg ttgccgaagt acaggcggaa ccgctcgaaa ccgcgatgtc gttaatgccc
     3541 atgagcagcg attcgccttc gacataggca aagctcatgt tcagattatt cggcaggcgg
     3601 tgctccatcg agccgttgat gaatacctca tccagcttgg cttccaggcc ctttcggaga
     3661 cgttcgcgca aggcgagcat acgtgccgct tcttccggca tctccttctg tgcgatttcg
     3721 caggccttgc cgaggcccac gataccaggc acgttcagcg tgccggagcg catgccgcgc
     3781 tcgtgtccgc cgccgtcgat gatggcggaa agctgtacgc ggggattgcg gcggcggacg
     3841 tacaaagcgc caacgccctt gggcccgtaa agcttgtgac cgctgatggc cagcaagtcg
     3901 atgttgtcct tctggacgtc gaccgggatt ttgcccacgg cttgcacgcc gtccacggcg
     3961 aagaagatac catgttcctt ggcaattttg ccgatttccg cgatcgggtt gatcacgcca
     4021 atttcgttat tggcgtacat gacggtgatc aggatcgttg tgggcttgat cgccgccttc
     4081 agtccgtcca ggctaacgcg gccgtcacgc tggaccggaa gataggtgac ctcaaagccg
     4141 tgcttctcga ggttcttgca ggtgtccagg acggccttgt gctcgatggc ctgggtgatg
     4201 atgtggttgc ccttttcgcg gaacatctcc gcgacgccct tgatcatcaa gttatcggat
     4261 tcggtggcgc cactcgtgaa gatgacttct ttcggcgtgg cgttgatcag cgaagcgata
     4321 agctggcgag aggtttccac ggcttcttcg ccggcccatc cgaaagaatg gttgcggctc
     4381 gcggcgttgc cgaatttctc ggtgaagtac ggcagcatcg cttcgacgac gcgcgggtcg
     4441 accggcgtgg tcgcgtgatt gtccatgtaa atcggcagct tgacggccat attccctctt
     4501 ctcttaagtc ctcagcgcta cgagcgcggg ctcgtgcggc tcttcgctca tctgggaaat
     4561 cgttacagtg ctcagcacat tcaaaatact ttcgttcacg cgacgcagcg gctcgcggac
     4621 cgtacacctc tccgtggcgt cgcagttccc gtggctcgta acacaggaag tgatcagcac
     4681 cggaccatcg atggccgaaa tcacatcaag cgcgctgatc tgccccggct ccttggccaa
     4741 ttggtagccc ccgctggaac catgcttcgc ggccaccagt gaatgatgcg ccagcttttg
     4801 cagcactttg gccatcagcg tcgcggagat gccatactgc tcggcaatct cgttggcact
     4861 gcaagcgtgc tgttgtcgat gcatcgccag atgcttgacg gcaatcagtc cgtaatcggc
     4921 tttcttggag agcttgaaca tcgttttcga gactttctgg acgctcagca atatgcgact
     4981 gaatcagtcg cacatgaagt atatccccag aggcatccga aagtcaaccc aaagccttcc
     5041 ttgccgtcaa tgcaacccct gtatttctag caacttatag taagaccaga acagtcttac
     5101 ttgctagtga atttcggcca acggtttcct gttgcagcga acggtggaaa ttccgagatg
     5161 aagtgcgaat cgctaagtgt gccgccgcaa catggtagcc tagactttcg atggaggaga
     5221 tgaatgctcg gatacatcgg gagatctaga gggttggcgg gccgatcagt gtgcggcctg
     5281 atcctagtgg tcctcctcgt tccgacacat gcccgagcgc agacacaatc gccttcgccc
     5341 accgatcgac ccaaacacgc ccaagtcaag gcatcgaaga aacggctatc ggaccgggct
     5401 tttgttctcg ttggcgcagg cgacatcgcc agttgccagg atccggaagg cgctcgggcg
     5461 acagccaagc tgatcgagca aatccctggg acggtttttg ccgcaggcga cctggcctac
     5521 gaaaagggga gcgcggaaga gttcaagaat tgctaccacc cggcttgggg tcgcttcaaa
     5581 gaccggacga aaccggcttt gggaaatcac gagtatgtag aacctacggc gtcgggttat
     5641 ttccgatact ggggcgcgca agcgggacca cagggcaagg gttactatag ttatgacctg
     5701 ggcgactggc acatcgtcgt gatgaatacc aattgcgacg ctaagaactt gggaggctgt
     5761 ggcgggggct caccgcagga gacgtggctg aaggaggata ttgcgaagca cccaaacgcg
     5821 tgcattctcg catacggcca ccacgcgctc ttcagcagcg gaatcttcaa gaatcacgcc
     5881 gtgcaccccg agttgaaaca actgtggcaa gacctgtacg ccgcccacgc cgacttggtt
     5941 cttgcgggac acgaacattc ctacgaacgc ttcgcgccac aggaacctga gggcaatgcc
     6001 gaccccgcgc acggaatccg cgaaatcgtc gctgggaccg gaggacgcag cctcgatctc
     6061 ctcggctttg ccactccgaa cagcgagatt cgggagtggg acacctacgg cgtgctgaag
     6121 ctcactcttt cgcctgggaa atacacttgg ggattcattc ccgaagaggg gaagtcattt
     6181 cgtgattccg gcagcggagt ctgtcacaac cagcccaccg aagcgaaata agtagccgtt
     6241 acatcggcac aattcacgcc taaggcttgg ggctagagca ggcaccaccc gcggcgggag
     6301 cctcgattag tttctgcacg gcgggtgcgg ggattagcag caactgtgcc ttcggctttt
     6361 tcacctgttc caagatggcg agctgctgcg ctttccacga atcaggagcc ggctgcccct
     6421 ttgcgatggc gtcgagtgca gcaagtccgg ctaacgccag cgttgagaga tcctgcgaac
     6481 ggccagcgac ttcctgcacc aggaaagagc gctgcgcgag gggctgaagc gctgcgtcat
     6541 tgtcgcgcca agccgtgagt tgtgcgcgga gccgcgattc cgccgcccga tcgtggcagg
     6601 cggaagcgag aaactgatcg accagatcgc caaaatgacg tcccgcatcg ctctcgagcg
     6661 gaaccgcatc cacgacgcga ttcatcggcg tggcgctggt cggctccgca ggagcggtct
     6721 gttcgcgcgt gtagtccttc accggttcga tgacgtcggt aagagtacgc agcgcggcaa
     6781 attccgtggc tgatgccgag cccgcgaggc gctgcagcat gtgccgcgaa gcggtccggt
     6841 gcgtgagtcc cagccactcc agccgggcac tgacaaaatc caaccgcgcg tacatggagg
     6901 cgggatcgcg gacttcctgc ggggaccaca agcgctcggc aatcgcggca ttcctgggcc
     6961 aaatgcgcga atccacgttt tccgggctga cgtactcagc ccacatgcac gattcaccgc
     7021 caaggactcg ctgcttctcc tccgcggaga ggtttgccgc gtccccgctc atcgggtcca
     7081 ctgcatagtg ccgcgccgcg gaccatccca gatccaagta gtatccgctc gacaaaatcc
     7141 cgcggtagcc ttgctttgcc gcttgcgcca gcgaagcttg cccgcgccag gactgaatca
     7201 caatatcctt cggcactcct gccaccagca cttcgtccca gccaaccaca gcttttccgt
     7261 gtttggtcac cagcttctgg acgcgttcgc tgaagtacgc ctgcagcgct tcgttgttct
     7321 tgatcccatg cgccttcatg aacgactgaa tctttggatt cgcgtcccac tccttcccgt
     7381 tcacctcgtc gccgccaatg tgaaaatagt ggtccggaaa aaccttggcc atctcgccga
     7441 tcaagtcatc gagaaacttg tagactttct cattcgtggg gtccattgcc ggatcgaaga
     7501 ttccccactt gcgctcgatt tcgtaaggtc cttttccgct ggccagctcg ggaagcccca
     7561 cgaaccacgc ggtgctatgc cccggcatat cgaattccgg caccacgcga attccccgat
     7621 cccttgcgta cgcgatcaga tcgcgaatct catcctgcgt gtagtaaaga gcgtcggagc
     7681 ccaattcgtg aagtttcgga aattttctac tctcggcgcg aaagccctgg ttctccgaga
     7741 ggtgccagtg gaaaacgttc atctttacag cttccatgcc gtcgatattc cggcggatga
     7801 cgtcgagggg ggtgaaatgg cgggcggaat cgatcatcaa gccgcgccac gggaaacgcg
     7861 gctggtcttg aatcttgagg gccggtgcgg caaatccgcc gggggaaaca tccaccaatt
     7921 gaagaaaggt ctggagccca tgcatcgcgc cgagcggcgt tggcgcgttg agcttcgcgc
     7981 ccgtggtggt cacttccaga atgtagctct cgtcctcgcc gacttcctga gtttccttgc
     8041 tcgcgtgatc ggtctgaatc acgagcgttg ctttggacgc cttggccggc ttgagggaga
     8101 gcgggagacc cgtttgacgc gcgagttgcc gcaagaatcg ctcgccggcg cgctccagcc
     8161 gcggatcggt gtagccggtg aacgccaccg aaaacgacga gtccactccc aggctccccg
     8221 tcccgggctg cgcgctcgcg ggaaggggca tcagattcaa ggtaggctgt tgagctgcag
     8281 tcatcgccgg gtacaacaaa acgcaaagaa caagtatgcc aagaccttgc aaccgcttat
     8341 tctgagccat ggtccggaaa atcctcctac gcagtgcgcc aaatctctga tggctgctat
     8401 gtagcaccag cttccgcata ctgtcatatc ttcgccgtcc tgcgaagagg attccttccg
     8461 ccgccgaaag aatccacttt ggcctcccac taagtttgca agctgcggat ttccccggct
     8521 gagttagaat aaacgcagac gtgcatcgcc gatgattcca cgatgaaaac ttcgattgcc
     8581 atttaccccg gttcgtttga cccggtcacc aacggacacc tcgacctcat cgagcgcggg
     8641 gagaagatgt tcgacctcct gatcgtggcc gtattgcgga atacggaaaa acagccgctg
     8701 ttcacggtta ccgagcgcat ggaaatgctg cgcgaggtca cgggaaaatg ggccgctgtg
     8761 gaagtggacg tgttcgaggg cctgctcatc aactatgcgc gcaagcgcgg cgccggcgtg
     8821 atccttcgcg gcattcgcgc cgtctccgac tacgaatacg aattgcagat ggcgctgatg
     8881 aaccgcaagc tcgagccgcg gctggagacc gtcttcatgc tgccgggcga gacgtatagc
     8941 tacttgagct cgaaactggt gcgggaaatc gcccagcatg gtggcccgct ggacggactg
     9001 gtgcccccga ccgtggagca aaggctgcgc gccaaggttt tgtaggtaaa acaggtgtaa
     9061 gttttctttt ttttactgag aaatttcaat tgtaaacttt taactttctt ctgggagcta
     9121 gagtgcaact aacagaccgc atcaaccgca tacaaatatc ggcgaccgcg gcggtgatcg
     9181 ccgaggccga caagctgaag tcgcgcggag tcgatatcgc agacttcggc cccggcgagc
     9241 cggactttcc cacgccggac cacatcaaaa tggcggcgat ccgggcgatc gaggaaaaca
     9301 agtcgaaata cacgccgaca ggcggcatca tgccgctgcg cgaagcgatt tgcgcgtggc
     9361 acaagcgcga actcggctcg tcgtacgaac cgaaagagtg cgccgtcagc gtcggcggca
     9421 agcactccat cttcaacgtg atgagcgtgc tgatccaaca gggcgacgag gtgattattc
     9481 cttcaccgta ctgggtgagc ttcccggaca tcgtaaaata tgcaggcggg acgccggtgt
     9541 tcgtggaaac caagccggaa gatggctttg cggtgcgcga ttccgccgtt gcaaaggcca
     9601 tcactcccag gacaaaactg ctgattctga attcacccag caatcccacc ggcggcgtcg
     9661 taccgccgga tgagtacgag cgcattctcg cgctttgcaa gaagcacaat gtttggctgc
     9721 taggcgacga gtgctattcg catttcacat acgagcccca caaaccgtac tcgatcgcca
     9781 g
//
LOCUS       DASYVO010000018         5149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_99126, whole genome shotgun sequence.
ACCESSION   DASYVO010000018 DASYVO010000000
VERSION     DASYVO010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99126"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VGR03_00745"
     CDS             complement(<1..237)
                     /locus_tag="VGR03_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992838.1"
                     /translation="MERVKAIQRNASAASEADAYRPELLADDEPRYLRRQKPVEIRRK
                     KFSGRGWPFYRWVLVLSFAGVAGLTVAVYGTRFLL"
     gene            complement(225..1133)
                     /gene="murB"
                     /locus_tag="VGR03_00750"
     CDS             complement(225..1133)
                     /gene="murB"
                     /locus_tag="VGR03_00750"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV1992839.1"
                     /translation="MRISDPRIVAKLAEMHVELQQGTALADLTSLGIGGTTDLLRIKK
                     HESLPDLLNLLDGNHVPHKFLGGGSNLLVGDGELPWVVLQLVHPKPDVVLTGNLAHVD
                     AAADLGRTITFCAKNDLGGMEGLIGVPGTVGGALRMNAGAYGMQIGSYVREVKLYRAA
                     SRKIEVLRGNQISFEYRHTSFAPDDMMLAVILELPSKPFKVILEGIRICNEKRRSSQP
                     LGQKSAGCIFKNPPGASAGRMIDELGLKGLSVGDARVSDRHANFFVNAGHASAKDMLT
                     LIADVRERVQKTYGVSLENEVVVWNA"
     gene            complement(1120..2529)
                     /gene="murC"
                     /locus_tag="VGR03_00755"
     CDS             complement(1120..2529)
                     /gene="murC"
                     /locus_tag="VGR03_00755"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV1992840.1"
                     /translation="MMVSFRNFQRIHLVGIGGIGMSGIAEVLLTLGYSVSGSDVKPST
                     ITERLQNLGATIFEGHKASNADGAHVVVTSSAVRADNPEVVEAHKNKIPVIPRAEMLA
                     ELMRLKYGIAVAGAHGKTTTTSIVASVLAAAHLDPTFVIGGKVNQAGTTARLGKGEYF
                     VVEADESDRSFLMLAPVVAVVTTIDREHLDQYTSLEDIQGAFIQFVNRVPFYGAAILC
                     IDEPNVQAIIPSVKRPIITYGTSSQADLVISDVKLEGLCSEFRLTYRGEDLGLFRLTH
                     PPGMHNVRNAAAAAAVALYLNVPADLIREGLVKFAGVGRRFDIKGVVNDITVVDDYGH
                     HPVEIRATLEAARGCKFNRLLVLFQPHRFTRTQHLWDEFCRAFNQADILVLIDIYAAS
                     EAPIPGVTSEVLASAIREAGHKNVHYFGSMQEGIEYLLKQARPGDAVLTIGAGNISRA
                     SNEFMVLLGTEHPTPHAHK"
     gene            complement(2526..3656)
                     /gene="murG"
                     /locus_tag="VGR03_00760"
     CDS             complement(2526..3656)
                     /gene="murG"
                     /locus_tag="VGR03_00760"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEV1992841.1"
                     /translation="MLRPYSARAPPVKLLIAGGGTGGHVFPALAIAQEWLSRGNEREV
                     VLVGTQHGIEMKLVPQAGLPLETLRVAGLKGKGGATLLKNLAMLAPAMLDARRVLRKH
                     KPVAAFGVGGYAAGPMLLATWLGRVPNVIFEPNAEPGFTNKVLARISSRIATGYEISA
                     RQWGKKAIVTGCPVRPEFFSITPRRLGKPFRLLVTGGSQGALPINRVFVDAMDRLAAR
                     KNELAIVHQTGERDYNALRTAYARREINAEVVPFLTNMAERFAWADVIVCRAGAITAA
                     EIAAAGRAAIFIPFGRATDSHQLRNAQEMTRAGAGRLISEVDLTAERLTGEIFSLLDQ
                     PQEIEKLSTAARGLARPYAARDIVDLIEEAANVQRNRQRASA"
     gene            complement(3703..4821)
                     /gene="ftsW"
                     /locus_tag="VGR03_00765"
     CDS             complement(3703..4821)
                     /gene="ftsW"
                     /locus_tag="VGR03_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEV1992842.1"
                     /translation="MPRRLENDRWLFGVTLALCLFGAVMIFSASAVTAEQQYGHSYIF
                     LVRQAAWLFIGLLGMFALMRTDYRRLREPAVIYPVVCVVLLLLVGTLFLDKSHATHRW
                     IKFGPVGIQPSELAKLATILYLAWFLDHKRRNKVSMKFCKEDFWRTIFPAAGPILVYV
                     ALILLQPDLGTSIDILVVATTILFVAGLSWKWIAVGLAGAIPVVYLLITQVAYRQARL
                     TVFLHPDSDPQGAGFQLLQSLIAVGSGGFTGVGLMESKQKLFYLPEAHTDFIYAVICE
                     ELGFIGAMIVIALFVVYGWRGMRAAFGAPDGFGRLLALGITAMVLCQALINFAVVLGM
                     VPTKGIPLPFISYGGSSLLVMLLATGVLLNISQQATEA"
     gene            complement(4828..>5149)
                     /locus_tag="VGR03_00770"
     CDS             complement(4828..>5149)
                     /locus_tag="VGR03_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992843.1"
                     /translation="GKDKGNDYTLLQRPLREKAILALLIGAAAEKIEKQITGSVAIER
                     AGMIERAVEIASHAARPGDVVLLAPACASFDQFQNYEHRGRVFKELVHQLERQAASTT
                     SGRG"
BASE COUNT         1055 a         1567 c         1509 g         1018 t
ORIGIN      
        1 cagaagaaaa cgcgttccgt agacagccac ggtcaaaccg gcaacgccgg caaaactcaa
       61 tacaagcacc caccgataga acggccagcc ccgcccgctg aatttcttgc ggcggatttc
      121 gaccggcttc tggcggcgca ggtaacgcgg ctcatcgtcg gcaagcagct cgggccggta
      181 ggcatcggct tctgacgccg cagacgcgtt tcgctggatg gccttcacgc gttccaaacg
      241 accacctcgt tctcaagact tacaccgtaa gttttctgca cgcgctcgcg cacgtcggcg
      301 atcagcgtga gcatgtcttt ggctgaggca tggccggcgt tcacaaagaa attggcatga
      361 cggtcgctga cgcgggcgtc gcccacagac aggcccttga gtcccaattc atcaatcatg
      421 cggcccgcgg aggcgcctgg agggtttttg aaaatgcagc cggcgctttt ctggccgagc
      481 ggctggctgg agcgccgttt ttcgttgcag atacgaatac cttcgaggat tactttgaac
      541 ggtttggagg gtagttccag aatcaccgcg agcatcatat catccggggc aaaagaagtg
      601 tgccggtatt cgaaagaaat ttgattgccc cgaagaactt cgatcttccg gctggccgcc
      661 cggtagagct tcacttcgcg gacataactg ccgatttgca ttccgtaggc cccagcattc
      721 atgcgcagag cgcccccgac ggtgccggga acgccgatga gcccttccat gccgccgagg
      781 tcgttcttcg cacagaaggt gatcgtgcgg ccaaggtcgg ccgcggcgtc gacgtgcgca
      841 aggttaccgg tgagcacgac atcgggcttt ggatggacga gttgcaggac cacccagggc
      901 agctccccat ccccgacgag caagtttgag cctccaccga ggaatttgtg cgggacgtgg
      961 ttcccgtcga gcagattcag aagatccgga aggctttcgt gcttcttgat gcggagcagg
     1021 tccgtcgttc caccgatgcc cagcgaagta aggtcggcaa gggccgtgcc ttgctgcagc
     1081 tcgacgtgca tttccgcgag tttggcaacg attctgggat cacttatgcg catgaggcgt
     1141 cggatgctcc gttcccagca gaaccatgaa ttcgttgctt gcccggctga tgttgcccgc
     1201 gccgatggta aggacagcgt ccccggggcg cgcttgtttc aacaggtact cgatgccctc
     1261 ctgcatggag ccaaaatagt gaacgttttt gtggccggcc tcgcgaatgg cgctcgcaag
     1321 cacttcgctc gtcacacccg ggatgggcgc ttcgctggcc gcatagatat cgatcagcac
     1381 gagaatgtcc gcctgattga aggcgcggca gaattcgtcc caaaggtgct gggtgcgggt
     1441 gaagcgatgc ggctgaaaga gcaccagcag gcgattgaac ttgcagccgc gagccgcttc
     1501 gagcgtggca cgaatctcga ccgggtgatg accgtagtca tcgaccacgg tgatgtcgtt
     1561 gaccacaccc ttgatgtcaa agcggcggcc cacgccggca aacttgacga ggccttcgcg
     1621 gatcaagtca gcaggaacgt ttaagtacag cgcgacggcc gcggcagccg ctgcgttgcg
     1681 gacattgtgc atgcccggag ggtgcgtcag ccggaacaag ccgagatctt cgcctcggta
     1741 ggtcaaccgg aactcgctgc acaaaccctc cagcttcaca tcgctgatga ccagatcggc
     1801 ttgactcgac gttccatacg taatgatggg acgcttcacg ctgggaatga tcgcctgcac
     1861 gttgggctca tcgatacaga gaatcgccgc accgtaaaat ggcacgcgat tcacaaactg
     1921 gatgaaggcg ccctgaatat cctcgagcga ggtgtactgg tcgaggtgtt cgcgatcaat
     1981 cgtagtcacc accgcgacca ccggcgcaag catcaggaag ctgcggtcgc tctcgtccgc
     2041 ttccaccaca aaatattcgc ctttgccgag ccgcgcggtc gtgcccgcct ggttgacttt
     2101 cccgccaatc acaaacgtgg ggtcaagatg cgccgccgcc agtaccgaag cgacgattga
     2161 ggtcgtagtt gtcttgccgt gcgcgccggc cacggcgatg ccatatttta accgcatcag
     2221 ctccgccagc atctccgcgc gcggaatcac cggaatcttg ttcttgtgcg cttcgacgac
     2281 ctcgggattg tccgctctta cggcggaaga ggtcacgacg acgtgtgcgc catccgcgtt
     2341 cgatgccttg tggccttcaa agatcgtagc gcccagattc tgcagccgtt cggtgatggt
     2401 cgatggcttg acgtccgagc cggataccga atagccgagc gtcaggagca cttcggcgat
     2461 accgctcatg ccgatgccgc cgatgcccac caggtggatg cgttgaaaat tgcggaaaga
     2521 aaccatcatg cgctggccct ctgccgattt ctctgcacgt tggcggcctc ttcgatgagg
     2581 tccacgatgt cgcgcgcggc ataagggcgg gcaagtccgc gcgcggcggt tgataatttt
     2641 tcgatctcct ggggttggtc gaggagagag aagatttcgc cggtcagtct ttcggccgtg
     2701 aggtccactt cggaaatcaa gcggccggcg cccgcgcggg tcatttcctg cgcgttgcgc
     2761 aattggtggc tgtcggtcgc tcttccgaaa gggatgaaga tggcggcgcg gcccgcggcg
     2821 gcgatttccg ctgcggtgat ggctcctgct cggcaaacga tgacatctgc ccaggcaaag
     2881 cgttccgcca tgttcgtgag gaagggaacg acctccgcgt tgatttcgcg ccgcgcgtag
     2941 gcggtgcgta aagcattata atcgcgttcg ccagtctgat gcacgatggc caattcgttc
     3001 ttacgtgccg cgaggcggtc catggcgtcg acaaataccc ggttgatcgg caacgcccct
     3061 tggctgccgc cggtgacgag cagccggaac ggttttccca gccgccgcgg tgtgatggag
     3121 aaaaactccg ggcgcacggg gcaacccgta acgatcgcct tttttcccca ttggcgtgca
     3181 gaaatttcat agccagtcgc aattcgtgaa gaaattcgcg ccaacacctt gttcgtgaag
     3241 cccggctcag cgttcggctc gaagatgaca ttgggcacgc gccccagcca ggtggccagc
     3301 aacatgggtc ccgctgcgta tccgccgacg ccaaacgcgg cgacaggttt gtgcttgcgc
     3361 aacacgcgcc gcgcgtcgag catcgccgga gcgagcatcg ccaaattctt gagcagcgtg
     3421 gcgccgccct ttcctttcaa gccggccacc cgcagcgttt ccagcggcag cccggcctgc
     3481 ggcacaagct tcatttcgat tccgtgttgt gttcccacta gcacgacttc cctttcattt
     3541 cctcttgaga gccattcctg cgcaatcgcc agcgccggaa aaacatgtcc gcccgtgccg
     3601 ccacccgcga tcaacaactt cactggcggc gctcttgccg agtaggggcg cagcatgctg
     3661 cgcccctaca accgaaattc gccaggcgtg gctctagttc cttcatgctt ctgtcgcttg
     3721 ttgggatata ttcagcagca caccggtggc cagcagcatg accagcaaac tcgatccgcc
     3781 atagctgatg aacggcaggg gaatcccttt cgtcggcacc atcccgagca ccactgcgaa
     3841 attaatcagc gcctggcaca agaccatcgc ggttattccc agcgccagca gccgcccgaa
     3901 tccatccggc gcaccgaacg ccgcacgcat ccctcgccag ccataaacga caaagagcgc
     3961 aatcacgatc atcgccccga taaatcccag ctcctcgcag atcactgcat aaataaaatc
     4021 cgtgtgcgcc tctggcaggt agaaaagctt ttgtttgctc tccatcaggc ctacgcccgt
     4081 gaagccacca gagccgacgg caatgagcga ttgaagcaac tgaaatcccg cgccttgcgg
     4141 atcggaatcg gggtgcagaa aaaccgtcaa gcgggcttgc cggtacgcca cctgtgtgat
     4201 gagcagatac accacgggaa ttgcgccggc gagtcccacg gcgatccatt tccacgagag
     4261 acctgcaacg aacaggatcg tggtggccac aacgagaata tcgatggacg tcccaaggtc
     4321 aggttgcagc aagatcagcg cgacatagac cagaatgggg ccagccgctg gaaaaatggt
     4381 ccgccagaaa tcttccttgc agaatttcat gctgactttg ttgcggcgct tatgatcgag
     4441 aaaccacgcc agataaagga tcgtggcgag tttcgccagt tccgaaggct ggatgcccac
     4501 cgggccgaac ttgatccaac gatgcgtggc gtgggatttg tcgaggaaaa gcgtgcctac
     4561 gagcaagagc aagactacgc agactacggg atagatgacc gcgggctcgc gcagacgccg
     4621 gtagtccgtc ctcatcaaag caaacatccc caagaggccg atgaagagcc aagcggcttg
     4681 gcggacgaga aatatgtacg agtggccgta ctgctgctcc gccgtgactg cggaggcgct
     4741 gaaaatcatc acggcgccga acaggcataa cgccaacgtc actccaaaga gccagcggtc
     4801 attttccagt cgtctcggca cgttcactca tcctcttccc gaagttgtac tcgccgcttg
     4861 ccgctccagt tgatgcacca gctctttgaa gacgcggccg cggtgttcgt agttctgaaa
     4921 ctgatcgaag ctggcgcatg ccggtgcaag gagcaccacg tcgccgggcc gcgccgcgtg
     4981 cgaggcaatt tccacggcgc gctcgatcat tccggcgcgc tcgatggcca cgctgccggt
     5041 gatctgtttt tcaatctttt cggccgccgc gccaatcagc agcgcaagaa tggccttttc
     5101 acgcaatggc ctttgcagca aggtgtagtc gttgcctttg tctttcccg
//
LOCUS       DASYVO010000019         3523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_42860, whole genome shotgun sequence.
ACCESSION   DASYVO010000019 DASYVO010000000
VERSION     DASYVO010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42860"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1577..3052
                     /locus_tag="VGR03_00775"
     CDS             1577..3052
                     /locus_tag="VGR03_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV1992844.1"
                     /translation="MATATTVMQPEVKIGPTKLLINGKFVNSVSGKTFPTINPSTGEV
                     ITQVAESDAADVDKAVAAARAAFEKGPWRKMGASQRGILMNKLADLIEKHADELALLE
                     ALDNGKPAHVARAADLPLTIACYRYYAGWADKIQGKTIPVNGNYFCYTKHEPVGVVGQ
                     IIPWNFPLLMQAWKLGPALAAGCTVVMKPAEQTPLTALRVGELMLEAGFPDGVVNLLP
                     GYGPTAGAALARHMDVDKVAFTGSTEVGHLIMKAAAETNLKRVTLELGGKSPNIVFAD
                     ADMDEAVEGSHQALFFNQGQCCCAGSRLFVEEKCYDEFVDRSVARAKKRTVGSPFDVK
                     TEQGPQVDQDQFNKVMSYIDAGKREKAKLATGGNRVGDKGYFIEPTIFSDVQDDMKIA
                     KEEIFGPVMTILKFKDLDEVVDRANKTMYGLAAAVWTKDITKAHAIANSVRAGTVWVN
                     CFDVFDAAAPFGGFKQSGIGRELGEYALSNYTEVKTVTVKL"
     gene            3097..>3523
                     /locus_tag="VGR03_00780"
     CDS             3097..>3523
                     /locus_tag="VGR03_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEV1992845.1"
                     /translation="MPLRFFAAAVVSFAAFFFAAQAGYGQSNSEPGIAQRHAQHLRYG
                     INLSEWFAQVYDAKGYTKEHFDAWTTAQDIGLIKSMGFDHVRLSVNPQPMFRRGQADR
                     IPADYLGYLDSAVQMILARGLAVIIDVHPDSDFKQKLVTD"
BASE COUNT          804 a          955 c         1113 g          651 t
ORIGIN      
        1 gagttcctcc agcatcattt ttcgcagatg ggtcacaggg acctccttgt gacccagaac
       61 ttatctcagc cgtcaagcaa ccactgctcc gttcacctca cgcgtagcgt tcgccgccgg
      121 gcggcttcgt tctgacgcgc aaaccgaaca tctggcgtgc tggaccgccc ccgaccacct
      181 atcacccatc ccttccggcg gttgacggga taagcgatag gaagatctga aactgccctc
      241 caccctgtgc tgggacagcc tcggacacat ttgcgaacgg cgggagtctt ggccttcagg
      301 ctgaaacgat ctcgggctat cgatcactac ttctacagaa gcggggagcg agaatgaggg
      361 tccggcgtgt atctcccatc tctgtaagat actcaacgcc aaatattccg tgagtaggga
      421 gggaaagatt caagggagcg gatcttagga ggtccggctc atgaccgaat ccgatcccag
      481 gaatcgacgc agaagccaaa gggtcacgct tcaagtcgcc gtacttatac gggcgaccct
      541 gctggatgga aggtccgtac aagtgcaagc gttcacctcg gcaataaatg cacacggcgg
      601 tctcttggaa tcgcctgtga aattggcagc caatcagaga atcctactca tcaacccgca
      661 ctcaggaaaa gatgttggtt gcagggtggt cggggctgag ggatcatcct cggccttgta
      721 cgaagtcgcc ttcgaatttg accgccgcag tccgcaattc tggccaatcc ctttcccacc
      781 agaggattgg gcggtgacgg aagaggcagg aaatgatagc cgctgaacgc ttgcgtacgc
      841 cacggctgga tccctgttca gaacagagct tcccaccagc taattgaccc agtgcctgat
      901 aagcgctaac cggacaagtg gatttttagc cgcacagtga caaaaccccc gcgccagtga
      961 cctcacaact cattgaaaga actggaccga tcactgagcc gtgatagggg gcaaaatcaa
     1021 tcgattctgc tacggcgagt gggagtccct taagtggcgg gtggcgcaat cgaaatccct
     1081 tgatcctgtg ttacgccacc cacttcatgg tgagtttctt gcgcttcttg gttcagcaaa
     1141 gaaagatgcc gggcttcccg acacacatcg tcgggacccc cacaattcgg gggaaagccc
     1201 agcgctacaa gggcggcgga gagggcaccg accttggcgg gtgctcctac caagaaatag
     1261 agatggcgag ataaactcgc cgctacaaaa tcgagagtgg agcgagccga caagatggca
     1321 ggctgaagcc cgccgccaca aatgctacct gcttgcctcc ggcggggcgc gggtataggc
     1381 ttcttcctcg caggacgttt gcgtcacgcg gggcttgagg ggtcttgctc cgcacctacc
     1441 agaagattgg gcaaacgtgc ttcggagagc cgccctaaag ggcggactat ataaccgaaa
     1501 atagaaagac gcccgcctcg gaaggcggcc gctacaacgg cgcttagggg cggcacgaaa
     1561 acaggaggta tttaaaatgg cgacggcaac gacagtcatg caaccggaag tcaagattgg
     1621 tccgacaaaa ctactgatca atgggaagtt tgtgaatagc gtatcgggga aaacgttccc
     1681 gacgatcaat ccgtcgaccg gcgaagtgat tacgcaagtg gcggagtccg acgcggcgga
     1741 tgtggacaag gctgtggcgg cggcgcgggc ggcgtttgaa aagggcccgt ggcgcaagat
     1801 gggcgcttcg cagcgcggca tcttgatgaa caagctggcg gacctcattg agaagcacgc
     1861 cgatgagctg gcactactgg aagcgctgga caacggcaag cctgcgcatg tcgcgagggc
     1921 ggccgatctt ccgctgacca tcgcctgtta ccgctactac gcgggctggg cggacaagat
     1981 tcaggggaag acgattcccg tgaacggcaa ctacttctgc tacaccaagc acgagccggt
     2041 gggagtggtc gggcagatca ttccgtggaa ttttccgctg ctgatgcagg cgtggaagct
     2101 agggccggcg ctggcagcgg gttgcacggt ggtgatgaag ccggcggagc agacgccgct
     2161 gacggcgctg cgcgtaggcg aattgatgct cgaggcgggc ttccctgacg gcgtggtaaa
     2221 cctgctgccc ggctacggac caactgcggg tgcggcgctg gcgcggcaca tggacgtgga
     2281 caaagtggcg ttcacaggat cgacggaagt gggccacttg atcatgaagg cggccgcgga
     2341 aacgaacctg aagcgcgtga cgctggaact cggcggcaag agcccgaaca tcgtatttgc
     2401 ggatgcggac atggatgagg ccgtcgaggg ttcgcaccag gcgctgttct tcaatcaggg
     2461 gcagtgctgc tgcgcggggt cgcggctttt cgtcgaagaa aaatgctacg acgaattcgt
     2521 ggatcgcagc gtggcgcgag caaagaagcg caccgttggc agcccgtttg atgtgaagac
     2581 ggaacagggg ccgcaagtgg atcaggatca gttcaataaa gtgatgagct acatcgacgc
     2641 cgggaagcgg gagaaggcga agctggctac tggcggcaac cgcgtcggcg acaagggcta
     2701 ctttatcgag ccgaccattt tctccgacgt gcaagacgac atgaagatcg cgaaggaaga
     2761 gatcttcggg ccggtcatga ccatcctcaa gttcaaggac ctggacgagg tcgtggaccg
     2821 cgccaacaag accatgtatg gactcgccgc ggccgtctgg accaaggaca tcaccaaggc
     2881 gcatgccatc gcgaacagcg tgcgcgcggg aacggtgtgg gtgaattgtt tcgacgtgtt
     2941 cgacgcggcc gcgccattcg gggggttcaa gcagtcgggg atcgggcgcg aactgggcga
     3001 atatgcgttg tcgaattaca cggaagtgaa aaccgtcacc gtcaaactat gaacgaacca
     3061 acaggacttg aagcacccgc aggggcacga tagatcgtgc ccctgcgttt ttttgcggcg
     3121 gccgtagttt ccttcgcggc gttttttttc gcggcgcaag ccggttatgg acaatccaat
     3181 tcagaaccgg gcatcgcgca acggcacgcc cagcatttgc gctacggaat taatttgagc
     3241 gagtggtttg cgcaggttta cgacgcgaag ggctacacca aagagcattt tgacgcgtgg
     3301 acgacagcgc aggacattgg gttgatcaag tcgatgggat ttgaccacgt gcggctgagc
     3361 gtgaatccgc agccgatgtt ccggcgaggg caggctgacc ggattcccgc ggattacctg
     3421 gggtatctcg acagcgcggt gcagatgatc ctggcgcgcg ggctggcggt gatcatcgac
     3481 gttcatccgg acagcgactt caagcagaaa cttgttaccg acg
//
LOCUS       DASYVO010000020         6823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_141263, whole genome shotgun sequence.
ACCESSION   DASYVO010000020 DASYVO010000000
VERSION     DASYVO010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6823
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141263"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /gene="meaB"
                     /locus_tag="VGR03_00785"
     CDS             complement(<1..802)
                     /gene="meaB"
                     /locus_tag="VGR03_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEV1992846.1"
                     /translation="MTPPVEKWAEQVRAGDVRAVSRAITAIENHQPEAEALLRLLFPH
                     TGQAYLTGVTGAPGTGKSTLVDRLATYHRKRQEQVGIIAVDPTSPYSGGAILGDRIRM
                     QGHASDAGTFIRSMATRGFLGGLARATAEVALLLDAAGKKHVLIETVGVGQDEIDIVR
                     LADCVLVVLVPGLGDDIQNMKAGLMEIGDIFVLNKADREGTDRLEQQLLAMLSLVMPR
                     DGWHPPVVRTVASENKGIEELAETVAEFQKHFESSGQRRTKHVEHWKNR"
     gene            complement(847..1995)
                     /locus_tag="VGR03_00790"
     CDS             complement(847..1995)
                     /locus_tag="VGR03_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEV1992847.1"
                     /translation="MEFSYTEEQQHLRRSVRGFAEGEIAPHVMEWDEASHFPMEIMPK
                     LAEMGLLGVIFPEQYGGAGLGYIEYALAIEELARVDGSVGLIVAAHNSLCSNHIFKFG
                     TEAQKKKYLVPLASGKAIGAWSLTEPEAGSDAGGTRTTAVREGNHWVLNGAKTFTTNG
                     HYANYCVAMAVTDKSKGSHGISAFILEKGTPGFRPGKKENKLGMRASDTSEVIFNDCR
                     VPQENLLGPEGEGFTGSLKILDGGRISIAALALGMAQGALEAATKYAKQRKQFGQAIS
                     EFQAVQFKLADMATQVEAARLLVYQAAWLADKKDVRFTRESSMAKLFASEIAVSVANE
                     CVQIHGGYGFIKDYPAEKYYRDVKLCTIGEGTSEIQKLVIARQLLGKK"
     gene            2172..2831
                     /locus_tag="VGR03_00795"
     CDS             2172..2831
                     /locus_tag="VGR03_00795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEV1992848.1"
                     /translation="MRRRHFLTLSAASLGGVLVYSLDRKASLLASEQKPIRIPLRFFT
                     ETEALIVAAAASRIFPSDDSGPGAREAGVAIYIDRQLAASYGRDRYRYTQPPFEDAPP
                     ELGYQAKETPRQIYREGLKGLKGFHLLPPAEQDSALQQIESSVFFALLRQNTIEGMFC
                     DPIHGGNAGMVGWQLVGFPGPRMSNYDEIEKYYGKAFRPTPVSLSQVTGKKIRPSEDA
                     P"
     gene            2828..4588
                     /locus_tag="VGR03_00800"
     CDS             2828..4588
                     /locus_tag="VGR03_00800"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEV1992849.1"
                     /translation="MKTLPAVDAVIIGGGWAGLLMAKELGSRTALSIVVLERGVSRNP
                     SEYADAMDELDYAIRMRMMQDLSQETVTLRHNASQRALPLRQHGSFQPGSGVGGSGEH
                     WNALCPRFLPDCFELLSRTTEKYGAKALPEDHSIQDWGVTYDELEPFYTRVDKLLGVS
                     GKAGNLKGKQIEGGNIFEGPRSEEYPTPPTKVPYFAALFRDAAKSLGYHPFPAPSATL
                     SQSYTNPDGISRGGCTYCGFCGRFGCMVGAKAQPTSLLLPIIQKRKNVVLRTGACVRR
                     ILFDSGSNSKARGVTYIDASGEELFQPASLVFLSSWTFNNTRLLLLSGIGEPYDPATG
                     RGTVGNNLTHQISTNAATAFFEQPLNRFMGSGSAAMFIGDFDGDVFDRGTLPFLRGGT
                     LGARGYGFQPIANFGVVPHSVKTKWGSEWKKAAVDYYDRTGVIGFTGEHLAYKSNFMD
                     LDPTYKDKLGDPLLRLTLDWRDNEKKMADFATTKAVEIARTMNAKEINPFSGLRSYDS
                     TRYQTTHVQGGTIMGNSPNHSVVNPYLQHWQLSNLFILGGSTFPQNPSMNPTPTILAL
                     TLRTADAIVDRFLKRPGLLA"
     gene            complement(4706..5830)
                     /locus_tag="VGR03_00805"
     CDS             complement(4706..5830)
                     /locus_tag="VGR03_00805"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEV1992850.1"
                     /translation="MSYTVARGNQRVDPDQMAEAGKMPAGVVDLRSDTVTRPTAEMRR
                     AMAEAEVGDDVYGEDPTVNRLEKRAAVIFGKEAALFVPTGCMGNLIGIKVWTHHGDEV
                     ICEERSHVNLYELASMSAIAGCMPRATRGEDGILTWKQIEAVIRPKIYYDSQTALVCL
                     ENTANMAGGTVYPTAQLEDICDHAHAAGLKVHLDGARIFNAATALGDNVAHMTRKVDS
                     VMFCLSKGLGAPVGSMVAGTKAFIEKARVYRKMFGGGMRQAGVIAAAGMIALEKSPAR
                     LHVDQENAKRLAEGIADIPGLSIDPKKVRSNIVIFDCSKTGKTAVELCDALHPRGVWA
                     QDTALHSVRFVTHCDVDRAGIERALIALREVAARPQSAGA"
     gene            complement(5899..>6823)
                     /gene="hemW"
                     /locus_tag="VGR03_00810"
     CDS             complement(5899..>6823)
                     /gene="hemW"
                     /locus_tag="VGR03_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEV1992851.1"
                     /translation="KVPASEGGFYKGVREDLMEVTLEADPETVEAGKAAAWVRAGINR
                     VSFGLQSFSDKELIAAGRMHRRADIYRAVPILREAGIRNISFDLIAGLPHQTKESWRQ
                     SLEEMAVLAPEHVSVYLLEIDEGSRLGKELLQGGGKYSAGAVPSEDEMADFYEMAQEA
                     LGAAGYHHYEISNWAKPGYESKHNLKYWRREAYLGFGAGAHSFSGTERWANAHDAAGY
                     VSAVQGGRLPVEQHETLTAESALEEELFLGLRQLDGIDLGRIERDYGVTLTARFDPLA
                     SAGLVKRDGNVVRLAPEKLSISNEVFVELMR"
BASE COUNT         1320 a         2427 c         1594 g         1482 t
ORIGIN      
        1 aacggttttt ccaatgctcg acgtgttttg tccggcgctg gccgcttgat tcaaaatgtt
       61 tttgaaattc cgcgaccgtc tcggccaatt cttcaatacc tttgttctcc gaagccaccg
      121 tgcgcaccac cggaggatgc cagccgtcgc gcggcatcac cagcgacagc atggcgagca
      181 attgctgttc cagccggtcc gtgccttcgc gatcggcttt gttcaaaaca aaaatgtcgc
      241 caatttccat gagcccggct ttcatgttct gaatatcgtc gccaagcccc ggcaccagca
      301 cgacgagcac gcaatcagcc agccgcacga tgtcgatttc gtcctgtccg acgccaaccg
      361 tctcgatcag cacgtgcttc ttccccgccg cgtccaggag cagcgccact tccgctgttg
      421 ctcgggcgag gccgccgagg aatccacgag ttgccattga gcggataaac gtgcccgcgt
      481 cgctggcatg gccctgcatg cgaatccgat cgcccagaat ggcgccgccg gaatacgggc
      541 tcgtgggatc taccgcgatg ataccaacct gttcctgccg cttgcggtga taggttgcca
      601 gacggtcgac gagtgtgctc ttccccgttc ccggcgcgcc agtgactccg gttagatatg
      661 cctgacccgt gtgcggaaag agcagccgga gaagcgcctc cgcttcaggc tggtgatttt
      721 cgatcgcggt gattgcgcgc gacaccgcgc gcacatcgcc ggcgcgcacc tgttccgccc
      781 acttctcaac gggcggtgtc atacttttcc tgtggagctt tatggagaag taggcgcatc
      841 tccctattat ttcttgccga ggagctgccg ggcaatcacc aacttctgaa tttcgctggt
      901 gccttcgccg atggtgcaga gcttcacgtc gcgataatac ttctccgctg gataatcctt
      961 gatgaagcca tagccgccgt gaatttgcac gcattcgttg gccacgctca cggcgatttc
     1021 gctggcaaag agtttcgcca tgctcgattc gcgcgtgaac cgcacgtcct ttttatccgc
     1081 cagccacgcc gcctgataaa ccagcagccg cgccgcttcc acctgtgtgg ccatgtccgc
     1141 cagtttgaat tgcaccgcct gaaactcgct gatcgcctgg ccaaactgct tcctttgctt
     1201 cgcgtatttc gtggccgctt cgagcgcgcc ctgagccatg cccagcgcca gcgccgcgat
     1261 cgaaattctt cccccatcca gaatcttcaa gctgccggta aatccctcgc cttccggccc
     1321 gagcaagttt tcctgcggca cgcggcaatc gttgaaaatt acttccgagg tgtcgctcgc
     1381 gcgcattccc agcttgtttt ctttttttcc gggccggaat cccggcgtcc ctttttctag
     1441 aatgaacgcg ctgatcccgt gtgaaccttt cgacttatcc gtgaccgcca tggccacaca
     1501 ataattcgca tagtggccgt tggtggtgaa cgttttcgct ccgttgagca cccaatgatt
     1561 gccttcgcgc acggccgtcg ttcgcgtgcc gcccgcgtcc gaaccagcct ccggctcggt
     1621 cagcgaccac gccccaatcg ctttccccga agccagcggc acaagatact tcttcttctg
     1681 cgcctcggtg ccgaacttga aaatgtggtt ggagcacagc gaattgtggg ccgccacgat
     1741 caagcccacc gagccgtcca ctcgcgccag ctcttcaatc gccagcgcgt attcgatata
     1801 gcccagcccc gccccgccat actgctccgg aaaaataacg ccgagcagcc ccatctctgc
     1861 cagcttaggc atgatctcca ttggaaaatg ggacgcctcg tcccactcca tcacgtgcgg
     1921 cgcgatttcg ccctcggcga atccccgcac gctgcggcga aggtgctgct gttcctcggt
     1981 gtagctgaat tccacggtct ctccggtgcg actatgtgct ctcgctatac tcaatccaaa
     2041 cataatttat accatagacc tcgcttcccc aaattgcccg ccttcccaac gtgccccggt
     2101 cgttcacttc catccacaga atctcacgaa ttcttcttct cctcattcgc gcctctgcta
     2161 gactcctgcc catgcgccgc cgccacttcc tcactctttc ggcagcaagc ctcggcggcg
     2221 tcctcgttta ttctctcgat cgcaaagcct ctctgctcgc ctccgagcaa aaaccaatcc
     2281 gcatcccgct ccgcttcttc accgagacgg aagctctgat cgtcgccgct gcagcttcgc
     2341 gcatcttccc atccgacgac tccggccctg gcgctcgcga agcgggcgtc gccatctaca
     2401 tcgaccgtca gcttgccgcc tcttacggtc gcgaccgcta ccgctacacg caaccgccgt
     2461 tcgaagacgc cccgcccgaa ctcggctatc aagccaaaga gacgccgcgc cagatctacc
     2521 gcgaaggact gaagggcctg aaaggttttc atctccttcc ccccgctgag caggacagtg
     2581 ccttgcagca gatcgaatcc tcagttttct tcgccctcct tcgccaaaac accatcgagg
     2641 gaatgttctg cgatccgatc cacggcggaa acgccggcat ggtgggctgg cagcttgtcg
     2701 gcttccccgg cccgcgcatg agcaactacg acgagattga gaagtattac gggaaggcct
     2761 tccgccccac gcccgtcagc ctgagccaag tcaccggcaa aaaaattcgt cccagcgagg
     2821 acgcgccatg aagactctcc ccgccgtgga cgccgtgatt atcggtggag gttgggctgg
     2881 cctgctcatg gccaaagaac tcggctcgcg caccgcgctt tccatcgtgg tcctggagcg
     2941 cggggtttca aggaatccat ccgagtacgc cgacgccatg gacgaattgg attacgccat
     3001 ccgcatgcgc atgatgcagg atctctccca ggaaaccgtc acgcttcgcc acaatgccag
     3061 ccagcgagca cttccgctgc gccagcacgg ctctttccaa cctggctccg gcgttggcgg
     3121 ctccggcgaa cactggaatg ccctctgccc ccgctttctt cccgattgtt tcgaattgct
     3181 ttcacgaaca accgaaaaat acggcgcgaa ggcgctcccc gaagatcact ccatccaaga
     3241 ttggggcgtg acctacgatg aactcgagcc tttctacacc cgcgttgaca aattgctcgg
     3301 cgtctccggc aaagctggca acctgaaagg aaaacaaatc gagggcggca atatttttga
     3361 aggaccgcgc agcgaggaat atcccactcc acccaccaag gtcccctatt ttgccgctct
     3421 cttccgcgac gccgctaaat cacttggcta tcatcccttc ccagccccat ccgcaactct
     3481 gagtcagtcc tacaccaatc ccgacggtat ctcgcgcggc ggctgcactt actgcggatt
     3541 ctgcgggcga tttggctgta tggtcggcgc aaaagctcag cccacttccc tcctcttgcc
     3601 catcattcaa aaacgcaaga acgtcgtcct tcgcaccggc gcctgcgtgc gccgtattct
     3661 gtttgattcc gggtcaaact ccaaagctcg cggtgtaacc tacatcgacg cctctggtga
     3721 agaactcttt caacccgcgt ccctcgtttt cctctcttcg tggacgttca acaacacccg
     3781 cctgctcctt ctctccggca tcggcgaacc gtatgaccct gcaacgggcc gcggtaccgt
     3841 tggaaacaac ctcactcacc agataagcac caacgccgcc actgccttct tcgaacagcc
     3901 gctgaaccgc ttcatgggct ccggttccgc ggcgatgttc attggcgatt ttgatggaga
     3961 cgttttcgat cgtggcactc ttccattttt acgcggtggc acactcgggg cgcgaggcta
     4021 tggcttccag cccattgcaa atttcggagt cgttccgcat tccgtgaaaa ccaagtgggg
     4081 ttccgaatgg aaaaaagccg cggttgatta ttacgatcgc acgggagtca ttggatttac
     4141 cggcgaacat ctcgcctaca aatccaattt catggatctc gatcccacct acaaagacaa
     4201 gctaggcgac ccacttctcc gcctcaccct ggattggcgc gacaacgaaa agaaaatggc
     4261 cgacttcgct accaccaaag ccgtggaaat cgctcgcacc atgaacgcaa aagaaatcaa
     4321 tcccttttcg ggcttgcgca gttacgactc cacgcggtat cagactacgc acgtccaagg
     4381 cggcactatt atgggcaact cacccaatca ctccgtcgtg aacccctacc tgcagcactg
     4441 gcaactctcg aatctcttca ttctcggcgg ctcgacgttc ccgcaaaatc cctcgatgaa
     4501 ccccactcct accattttag cgctgacgct ccgcaccgcc gacgccatcg tcgaccgctt
     4561 cctgaaaaga cccggcctcc ttgcctaatg ccttattgaa atactatttt ctgattgtca
     4621 ttccgagcgt agcgaggacc gcttcctctc cgcgtgctct tcgcgcggag aatctgcttt
     4681 tcgttaaata cgtgctcgct tgcttctacg ctcccgcgct ctgcggacgc gccgcaactt
     4741 ccctcagcgc aatcagcgct cgctcgatcc ccgcccggtc cacgtcgcaa tgcgtaacga
     4801 atcgcaccga atgcagcgcc gtatcctgcg cccacactcc gcgaggatgc agcgcatcgc
     4861 acaactccac ggcggttttc cccgtcttcg agcaatcaaa gatcacgatg ttcgaacgca
     4921 ctttcttcgg atcgatgctc agccccggga tatcggcgat accttccgcc agccgcttgg
     4981 cattttcctg atcgacgtgc agtcgcgccg gagacttttc cagcgcaatc atgcccgccg
     5041 ccgcgatcac gcccgcctga cgcatgcctc cgccgaacat tttccggtag acccgcgcct
     5101 tttcgatgaa cgctttcgtg cccgcaacca tcgatcccac cggcgcgccc aatcccttcg
     5161 acaaacagaa catcaccgaa tccacttttc gcgtcatgtg cgcaacgtta tctcccagcg
     5221 ccgtcgccgc attaaaaatc cgcgccccgt caaggtgcac tttcaagccc gcggcatgtg
     5281 cgtggtcgca aatgtcctcc aactgcgccg tcggatagac ggttcctccc gccatgttgg
     5341 cggtgttttc caggcatacc agcgccgttt gcgaatcgta ataaatcttc ggccggatga
     5401 ccgcctcgat ctgcttccac gtcagaatcc catcttcccc gcgggtcgct cgcggcatgc
     5461 agcccgcaat cgccgacatc gacgccaact catacagatt cacgtggctg cgctcttcgc
     5521 aaatcacttc gtcgccgtga tgcgtccaca ccttgatccc gatgagattc cccatgcacc
     5581 ccgtgggaac aaacagtgcc gcctctttcc caaaaatcac cgccgcccgt ttttccagcc
     5641 ggttgaccgt cgggtcttct ccatacacgt catccccgac ctccgcctcc gccatcgccc
     5701 gccgcatctc cgccgtcggc cgcgtcaccg tatcgctccg caggtccacc acgcccgccg
     5761 gcatcttccc tgcctccgcc atctgatccg gatcgaccct ctgattcccc ctcgccaccg
     5821 tatagctcat gctcttctcc gtgttctccg tgttaaaagc gtttgctgac tgcaagctaa
     5881 gtcaacctgt tccgagtatc accgcatcaa ctccacgaac acttcatttg agatactcag
     5941 cttctccgga gcaagccgca ccacgtttcc gtctcgtttc accaaacccg ccgatgccag
     6001 cggatcaaac cgcgccgtga gcgtcacccc atagtcccgc tcgattcgcc caagatcaat
     6061 tccatccagc tgccgcaacc cgagaaacaa ttcttcctcc agcgcgctct ccgccgtgag
     6121 cgtttcatgc tgctccaccg gcagtcgccc gccctgcacc gcactcacat atcccgccgc
     6181 atcgtgcgcg ttcgcccatc gctccgtccc cgaaaacgaa tgcgcccccg ccccaaatcc
     6241 cagatacgcc tcgcgtcgcc aatatttcag gttgtgcttc gactcatatc cgggcttagc
     6301 ccaattcgaa atttcgtagt gatgataccc cgccgctccc agcgcttcct gcgccatctc
     6361 ataaaaatcc gccatctcgt cttcgctcgg caccgcccca gcgctgtact ttcccccgcc
     6421 ttgcagcaac tctttgccca gccggctccc ttcatcaatc tccagcaagt aaacggaaac
     6481 gtgctccggc gccagcaccg ccatttcctc caaagactgc cgccagcttt ccttcgtctg
     6541 gtgcggcaac cccgcaatca aatcaaagct gatatttcga atacccgcct cgcgcagaat
     6601 cggcaccgcg cggtaaatat ccgcccgccg atgcatccgc cccgccgcaa tcaattcctt
     6661 atccgaaaac gattgcagcc caaaactaac ccggttgatc cccgcacgca cccaagccgc
     6721 cgcctttcca gcctcaaccg tctccggatc agcctccagc gtcacctcca tcaaatcttc
     6781 tcgaacgcct ttgtagaagc cgccttccga cgcgggcacc ttg
//
LOCUS       DASYVO010000021         4387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_254716, whole genome shotgun sequence.
ACCESSION   DASYVO010000021 DASYVO010000000
VERSION     DASYVO010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254716"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VGR03_00815"
     CDS             complement(<1..933)
                     /locus_tag="VGR03_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEV1992852.1"
                     /translation="MAAHSHDDDHSRAGHSHSHGPTHAGHAHPTLRPSVLGWAMAATL
                     GLVVAEVFGGILGRSVALLNDAVHNLSDVPALGISYLAMRWAQRPADSEKTYGYHRAG
                     TLAAYTNAFVLVLLSLWLGYEAFERFRSPVEVVESWMIWTSVGALAVNGGITLALVRG
                     RGDLNLRSILVHNFGDALSNIAIIGGALIIRATGARWIDPLLGLAIGLLVLWSSTGIL
                     RESSHILLEGRPLDLRVEDVARAILTIEGVQEVHDVHLWSLGGGHNALSLHARIPDMH
                     LDECERLLASIKQVASKEFGVEHTTVQLERAGLPA"
     gene            1120..2289
                     /locus_tag="VGR03_00820"
     CDS             1120..2289
                     /locus_tag="VGR03_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1992853.1"
                     /translation="MTVPIAKLSFWGVRGSTPTVDPATWRYGGNTPCLELIAPDGTQF
                     ILDCGTGLRMLGSRWAAPNGGKAPETHILVTHYHWDHIQGIPFFSPLYVEKNEFHFYS
                     FRSKYLGRDSLKQVFEAQMALPYFPVDMSAMSAKRKFKEVDGGDSFTVGENKITTRWL
                     NHPQGCLGFRIETPAGTVAYATDNEPGDAKLDESLRELAAGADIFINDAQFTPEQLET
                     TRKGWGHSSWLEGAKVALQAGAKTLVLFHHDPDSTDRMVDSILRQAREEFDSVFAASE
                     GMVITLGAPGDTVQAHMPGTRTALRREAQFHAKVCGVTEGGKDFEEETVVRDISLQGA
                     LISLKNLPRLQSELQVTMEAPGADGLQSMNLRGYVVRIDAGPEKGHVAVGVVFTD"
     gene            2403..3497
                     /locus_tag="VGR03_00825"
     CDS             2403..3497
                     /locus_tag="VGR03_00825"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV1992854.1"
                     /translation="MAEKKANFVPVSALDKRPPSKRDLLRGLVRQAPFVAKNFQLYFR
                     NAWRRHVLQTKVVAPYAVTFYVTHKCNLACSYCTQKEPDVFSDELPTRETIRLLKRIR
                     KETDSIVITGGEPTLRPDIEELVEAARFECKYRSLLLITNGTLLDRKPRILRAVTGLV
                     VSLDALATDPTNPLSKPAALPKVLENLELAKELLGSPRRITISTVIEQWNVGEVERLL
                     DWCGEQGFVFAAQSAQMEKMPNLALLKNPKYQALVDKIIERRRKGVQPINGTPRTIET
                     LLRFGDFQCYPTMFPRVYPNGDVFYPCEPLHKIAGNLLRNGSLKKIFARGKKIYGDIP
                     PCQGVCYLFGNVLSHYYVNDFWGLAGDFIR"
     gene            complement(3723..>4387)
                     /locus_tag="VGR03_00830"
     CDS             complement(3723..>4387)
                     /locus_tag="VGR03_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992855.1"
                     /translation="AAIKPSQNEPVQHELARLARILRYLYDGTNLGAIACAEIAAKAL
                     LQEEPHVVLARETVDSLETHFRRGPFAAMLRGGSAPARVILGLGLLLYIGLPSGYFLF
                     RRISGIKEILGINVSMLIGVALAGALGSIVSIMVRLQDFVSVHVKDPTVVFFTGFFKP
                     IVGMSFAMFVFACLNAGILPLAIKVDTPAAGYFFLALGFVSGFSERFAQDVASRAEKS
                     IK"
BASE COUNT          987 a         1170 c         1370 g          860 t
ORIGIN      
        1 tgccggcaag ccggcgcgct ccaactgcac ggtggtgtgc tcaactccaa attcctttga
       61 agctacttgc ttgatcgatg cgagcaagcg ttcacactcg tcgaggtgca tatccggtat
      121 gcgcgcgtgg aggctgagcg cattgtggcc gccgccgagg gaccagagat gcacgtcgtg
      181 gacttcctgc acaccttcga tggtcaggat tgcgcgggcg acatcttcca cgcgcagatc
      241 aagcgggcgg ccctccagaa gaatgtgcga ggattcgcgc aggatgccgg tgctggacca
      301 gaggactagc aggccgatgg ctaggccgag aagcggatcg atccaacggg caccggtcgc
      361 acggatgatt agcgcgccgc cgatgatggc gatattcgag agcgcatcgc caaaattgtg
      421 aacgaggatg ctgcgcagat tgaggtcgcc ccgcccgcgg acgagagcga gcgtgatgcc
      481 gccgttgacg gcgagcgcgc cgacggaagt ccagatcatc cagctttcga ccacttccac
      541 gggggaacgg aaacgttcga aagcttcata gccgagccag agcgaaagca ggacgaggac
      601 gaaggcgttg gtgtaggcag cgagtgtgcc ggcgcggtga tagccataag tcttttcgct
      661 gtcagcgggg cgctgggccc agcgcatggc gagataggaa atgccgaggg cgggtacatc
      721 ggaaagattg tgaacggcgt cgttcagaag ggcgacgctt ctgccgagaa tgccgccaaa
      781 aacctccgct accaccaaac cgagagtggc ggccatggcc caaccaagca cagaggggcg
      841 cagcgtgggg tgagcgtggc cggcgtgagt ggggccgtgg gaatgcgaat ggccggcgcg
      901 actgtggtca tcatcatggg aatgggcggc catctgcctt attctacccg ctcaatggcg
      961 gcggcgcagc cctttcccat gcttattggg cagtatgaca cttcacctcc gcgaattgat
     1021 tgacgctccc tccatcggct gcgaccatga aacgagcttc tcgttccgtg cttgttcgtg
     1081 gggaacgagc cttcacacag gggtattgcg ctcagggcaa tgacggttcc catcgcaaaa
     1141 ttgagtttct ggggcgtgcg cggatctaca ccgacggtag acccggcgac gtggcgctat
     1201 ggcggaaata cgccctgtct ggagctgatt gcgccggacg gcacgcaatt cattctggat
     1261 tgcggcacgg ggctgcgcat gctggggagc cgatgggccg cgccgaacgg cggaaaagct
     1321 ccggaaacgc acatcttggt cacgcactat cactgggatc acatccaggg gattccattt
     1381 ttttcgccgc tatatgtgga gaaaaacgaa tttcatttct acagcttccg gtcaaaatat
     1441 ctggggcgcg acagtctgaa acaagtgttc gaagcgcaga tggcgctgcc ctattttccc
     1501 gtagacatgt cggcgatgag cgccaagcga aaattcaagg aagtggacgg cggcgattcg
     1561 ttcacggttg gagagaacaa aatcacgacg cgctggctga atcatccgca gggctgcctg
     1621 ggcttccgaa tcgagacgcc ggcgggcacg gtcgcttatg ccacggacaa cgagccgggc
     1681 gacgcgaaac tggacgagag cctgcgcgaa ctggccgccg gtgcggacat cttcattaac
     1741 gatgcgcaat ttacacccga acagctcgag acgacgagaa aaggctgggg acattcctcg
     1801 tggctggagg gagcgaaagt ggcgctgcaa gccggggcga agaccctggt acttttccat
     1861 cacgacccgg attcgacgga tcgcatggtg gattcgattc tgcggcaggc gcgggaagag
     1921 ttcgattcgg tgttcgcggc gagcgaaggc atggtgatca cgctcggcgc accgggagac
     1981 accgtgcaag cgcacatgcc gggaacgaga acggcgctgc gccgcgaagc gcaattccac
     2041 gcgaaagtct gcggcgtaac cgaaggcggg aaggatttcg aggaagagac cgtcgtccgg
     2101 gatatttccc tgcagggagc attgatttcg ctgaagaacc tgccgcgact gcaatcggag
     2161 ctgcaagtca ccatggaggc gccgggcgcg gatgggttgc aatcgatgaa cttgcgtgga
     2221 tacgtggtca ggattgacgc gggaccggaa aaggggcacg tcgcggtggg cgtggtgttc
     2281 accgactaat tcggagtctc aagttagttg agtttctact taggggcgcc ccgattcgat
     2341 cggggcgccc ctacagtttt cttggcatgg ctgatttggt cctgtaggct gaagtggccc
     2401 ctatggctga gaagaaagcg aacttcgttc ccgtcagtgc gctggacaag cggccgcctt
     2461 cgaaacgcga tctcttgcgc ggattggtgc ggcaggcgcc gtttgtggcg aagaactttc
     2521 agctttattt ccgcaacgct tggcggcggc acgttttaca aaccaaggtc gtggcgccct
     2581 acgcggtcac gttttacgtc acccacaaat gcaatctggc ctgcagttac tgcacgcaaa
     2641 aggagccgga cgtcttcagc gacgagttgc ctacgagaga gacgatccgg ttgctgaagc
     2701 gaatccgaaa agagacggac tccatcgtga ttacgggcgg tgagccgacg ctgcggcccg
     2761 atatcgagga gttggttgag gcggcgcgtt tcgaatgcaa ataccgttcg ctgctgctga
     2821 tcaccaacgg cacgctgctg gatcgaaaac cgcggattct gcgagcggta acgggactgg
     2881 tagtgagcct ggatgcgttg gcgaccgatc ctacgaatcc gctctcgaag ccggcggcgc
     2941 tgccgaaagt gctggaaaac ctcgagctgg cgaaggagct gctgggcagc ccgcggcgaa
     3001 tcaccatcag tacggtgatc gaacaatgga acgtgggtga agtcgaacgg ctgctggatt
     3061 ggtgtggcga gcaaggattt gtttttgcgg cgcagtccgc gcagatggag aaaatgccga
     3121 acttggcgct gctgaaaaac ccgaagtatc aggcgctggt ggacaaaatc atcgaacggc
     3181 ggcgcaaggg tgtgcaaccg atcaacggca cgccgcggac gatcgagacg ctgctgcgtt
     3241 tcggcgattt tcagtgctat ccgacgatgt ttccgcgggt gtatccgaat ggcgatgttt
     3301 tttatccctg cgagccgctg cataagattg cgggcaattt gctgcggaac gggtcactga
     3361 agaagatctt tgcgcgcggg aagaagatct atggagatat tccgccgtgc cagggtgttt
     3421 gctacctgtt cgggaatgtg ctgtcgcact attacgtgaa tgatttttgg gggctggcgg
     3481 gggacttcat ccgctgaaga gaacggcaat aacgaaggat tggaaatgcg ggcacgtgag
     3541 cggcgtgcat tttgggtagt gggccaattt cagccgggga cgatgacaac ctccagccgc
     3601 catgccattt tccggacagc ggggtcactc aacgcagtgg attacttccg agtgatctat
     3661 gcacaaacag taaacttaca ttgagcattg cagagatacg agcattctca aaacataccg
     3721 actcatttta tggacttctc agcccgcgac gcgacatcct gagcgaacct ttcgctgaag
     3781 cctgagacga agcccaacgc cagaaaaaaa tacccggcag ctggagtatc caccttgatc
     3841 gccagcggga gaatgcccgc attgaggcat gcgaatacaa acattgcaaa gctcatcccc
     3901 acaatgggct tgaagaatcc cgtaaaaaaa accactgttg ggtctttcac atgcaccgaa
     3961 acaaagtctt ggagccgaac catgatgctc actatgctgc ccagtgcccc tgcgagcgcg
     4021 actccgatca acatagagac gtttataccc aggatctctt ttatcccaga gatccgccga
     4081 aacaagaaat aaccgcttgg taggccgatg tagagaagca agcctaatcc tagaatcacc
     4141 cgtgctggcg ccgagcctcc ccgcaacatc gctgcaaatg gaccccttcg gaaatgagtc
     4201 tccaatgagt caactgtctc tctcgcgagc accacatgcg gttcttcttg aagaagagcc
     4261 ttagcagcga tttcagcgca agctatggca ccaaggttcg ttccgtcata caaatatcgc
     4321 aagatcctag caagacgtgc tagttcatgc tgcactggct cgttttgact gggcttgata
     4381 gctgctt
//
LOCUS       DASYVO010000022         3025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_14898, whole genome shotgun sequence.
ACCESSION   DASYVO010000022 DASYVO010000000
VERSION     DASYVO010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14898"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..724)
                     /locus_tag="VGR03_00835"
     CDS             complement(14..724)
                     /locus_tag="VGR03_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992856.1"
                     /translation="MTPPARVALMKRFVLLNEPGKPTASANPAGRLILRCQTPDVTTE
                     MQIGGAELRDNIAFLPMELRDATDTTGASVHQITMGLVREDGDWKLLSLGLLLLDLPA
                     LEVEWDADEIGAAERNAIEALNKVAGAIEAYRNKYLRLPESLANLGPPLHGAANGEAA
                     GFLDSDLANGMKNGYTARYVIVGASALGAPAKYELAATPLQYGRTGRRSFFRDSNGAV
                     HAADRRGAVGSGTDPKVE"
     gene            complement(981..1331)
                     /locus_tag="VGR03_00840"
     CDS             complement(981..1331)
                     /locus_tag="VGR03_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV1992857.1"
                     /translation="MGRVVALMDDLFFQMKLAETAKQLGVEVKVATNCEALMGLMASE
                     PKLVIVDLNARSQPIAAIEKLRETRKDVRVVGFLSHVQRELAAQAQAAGCDEVLPRSS
                     FTQNLAAILSVAKD"
     gene            1440..1730
                     /locus_tag="VGR03_00845"
     CDS             1440..1730
                     /locus_tag="VGR03_00845"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEV1992858.1"
                     /translation="MSVHPEDACLAASGPRDVTLYTRPGCHLCDEAKSAIAPLLRELS
                     AALREVNIDDDPVLKERYGWDIPVIFIGQKKAAKHSVDLEQFRRQLQDAGAG"
     gene            complement(1770..2213)
                     /locus_tag="VGR03_00850"
     CDS             complement(1770..2213)
                     /locus_tag="VGR03_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992859.1"
                     /translation="MWNVEERFSPRAIESLAALGFAVGSTRGVVRVEKYGSGAEFRKG
                     TDDLYQMTIIPTIMLKGQFTRLWDAGYQKFLLTDEGLKLPALATHLQNLRKFNEELRT
                     ALGIPTFYNEALGSVSQVSVYDRVKGRKGDVPDETVGAHEAGGGH"
     gene            complement(2340..>3025)
                     /locus_tag="VGR03_00855"
     CDS             complement(2340..>3025)
                     /locus_tag="VGR03_00855"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEV1992860.1"
                     /translation="QAAKLIHRVNPAYPPLAAQAHVSGTVRLHAIIGKDGTVREVNAL
                     TGHPLLLQSAINAVKQWRYSPTRLNGRPVEVDTTVSVEYTLSSIPSTTAKSVPVPDPS
                     ASAPSSLPASAPPTQKGPCAFGKIEYTEQNNMLIGSVPYTYQGSYALDAVAVRGIALS
                     KEKKQVAGLTYAQSTLKTASGTAQFSIEGHPSLGRQGEASDYLLIAIVVKQTGATVCG
                     EIVPYQRKW"
BASE COUNT          567 a          912 c          881 g          665 t
ORIGIN      
        1 ctccaaggag gttctactcc actttgggat ctgtcccgct gccgaccgcg cctcgccggt
       61 cagcggcgtg cacagcaccg ttcgaatcgc gaaagaacga gcggcgtccg gtgcgcccgt
      121 actgcagcgg agttgccgcc agctcgtatt ttgccggagc accgagggcg ctcgcgccga
      181 caatcacgta acgagcggtg tagccgtttt tcattccgtt cgctaggtcg gaatcgagga
      241 acccggcagc ctcgccattc gccgcaccgt gaaggggtgg ccccagattc gccagagatt
      301 cgggcaggcg caaatatttg ttgcggtagg cttcgatggc gccggcaact ttgttcaacg
      361 cttcgatcgc gtttcgctcc gccgctccta tttcgtcggc atcccattcc acttccagtg
      421 cgggaagatc caggagaagc aagcccagcg agagcagctt ccaatccccg tcctcgcgca
      481 ccagccccat ggtgatctga tgcacgcttg cgcctgtggt atcggtggcg tcgcggagct
      541 ccatcggaag gaacgcaata ttgtctcgaa gttcggcgcc gccgatctgc atctccgtgg
      601 tgacgtcggg agtttggcag cgaagaatga ggcggccggc gggattcgcc gaggccgtgg
      661 gtttgcccgg ctcattcaga agcacgaaac gcttcatcaa cgcaacgcgg gctggcggcg
      721 tcatgcggga aaacgcctct ctgtttcgag ccgtgaggaa gcgaataaac tcggtttgat
      781 tctgggaaca tgccgcggac agcaccgctt tcagcgccgc gctgggtccc ccagtatttg
      841 ccgtttcgct cggcacgctc gcggagcttc caccgggctt agaactggat tcctgtgaaa
      901 gggcgttcgc ggctgcgagc agaacggcgc agctcaggaa gaagacttgt ccgcggacct
      961 tcgatttccg tgatttcacg tcaatccttt gccacgctca gaatcgccgc gagattctgt
     1021 gtgaaactcg agcgcggcag aacttcatcg catcccgccg cctgcgcttg agcagcaagt
     1081 tctctctgca cgtgcgacag gaagccgacc acgcgaacgt ccttgcgcgt ctcccgcagc
     1141 ttttcaatcg ccgcaatcgg ctggctccgc gcgttcaagt ccacgatgac aagttttggc
     1201 tcactcgcca taagccccat caaggcctcg cagttcgtcg ccacttttac ttccacaccg
     1261 agctgcttgg cggtttccgc cagcttcatc tggaaaaaca aatcgtccat cagcgccacg
     1321 actcttccca tttcggtccg cctcttttta gctgctccgc aacaactgcc ctgttattat
     1381 gcgcccgttc ttcccaacgg cgactgttcc ccggtaagat gcacgttcgc acttaaacga
     1441 tgagcgttca tcccgaagac gcctgccttg ccgcttccgg ccctcgggac gttactctct
     1501 acacgcgtcc cggatgccat ctctgcgacg aagccaagtc cgccatcgcg ccgctcttgc
     1561 gagaattaag cgccgccctc cgggaagtca atatagatga cgatcctgtc ctgaaggagc
     1621 gttacggttg ggatattccc gtgatcttca tcggccagaa gaaagcggcg aagcatagtg
     1681 tggatcttga gcaattccgg cggcagcttc aggatgccgg ggcaggctga aatcctcaac
     1741 tagaatttcg tggaaaagga gcggcgatcc tagtggccgc cgccggcttc gtgcgcgccg
     1801 acagtttcgt ccggcacgtc gcctttgcgg cccttgacgc ggtcgtaaac gctgacctgg
     1861 ctgacggaac cgagtgcttc gttgtagaag gtcggaatgc ccagagccgt gcgaagttct
     1921 tcgttgaact tccgcaggtt ttgcagatgc gtcgcgagcg ccggcaattt cagtccttcg
     1981 tcggtgagca aaaacttctg gtaaccggcg tcccacaacc gcgtgaactg gcctttcagc
     2041 atgatggtgg gaataatggt catctggtag aggtcatcgg tgcctttacg aaactccgcg
     2101 ccgctgccgt atttttcgac gcgtaccacg ccgcgcgtgc tgcccacggc aaaccccagc
     2161 gccgcgagac tctcgattgc tcttggtgag aatcgctctt cgacattcca catgatggat
     2221 tcagaatacg atgctcgtcg cgcgtctgta aagacagacc agcatcgtgg atttctcatt
     2281 ggtcgtttta tgttcttatt gcgggataca tatcgcgccg gcgaaggccg gatgagcact
     2341 taccacttcc gctggtaggg gacgatttct ccgcagacgg ttgcccccgt ttgcttcacg
     2401 acgatggcga tgagcaagta gtcgctcgct tcgccctggc ggcccagcga ggggtggcct
     2461 tcaatcgaaa actgagcagt gccgctggcg gtcttcaagg tggattgcgc ataggtgagc
     2521 cctgcaacct gcttcttttc tttcgatagc gcaatcccgc gcacggctac cgcatcgagg
     2581 gcatagctgc cctgataggt atagggcacg gagccgatta acatattgtt ttgctccgta
     2641 tactcgatct ttccgaatgc gcatggacct ttctgcgtgg gcggcgcact tgcgggcagc
     2701 gaactgggag ctgaggcaga cgggtcagga acgggaacgc ttttcgccgt cgtggaagga
     2761 atacttgaaa gcgtgtactc gacactaacg gttgtatcca cttcgacggg tcggccattc
     2821 aagcgcgtcg gcgagtagcg ccattgcttt accgcattga tggcggactg caaaagcaac
     2881 gggtgcccgg tgagcgcgtt cacttcgcga acggttccgt cctttccgat gatcgcgtgc
     2941 aggcgcaccg ttcccgagac atgcgcctgc gcggccagcg gcgggtaagc gggattcacg
     3001 cggtggatga gttttgcggc ctgta
//
LOCUS       DASYVO010000023         6561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_127990, whole genome shotgun sequence.
ACCESSION   DASYVO010000023 DASYVO010000000
VERSION     DASYVO010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127990"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..379)
                     /locus_tag="VGR03_00860"
     CDS             complement(179..379)
                     /locus_tag="VGR03_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992861.1"
                     /translation="MAAFFLWIILFILCWPLALLALVLYPIVWLLTLPFRLVGIAVHG
                     VLGLFSAIFMLPVRLLRGPRAI"
     gene            complement(455..1846)
                     /gene="hflX"
                     /locus_tag="VGR03_00865"
     CDS             complement(455..1846)
                     /gene="hflX"
                     /locus_tag="VGR03_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV1992862.1"
                     /translation="MTKTQHQVSTAERALLVGVGWKRAPRFPGMPAGEQGRESLSELV
                     ELARSAGAEIAATVFQMRDTADPATLVGRGKLDEIRAEATAHKAPLIIFDSNLSPVQQ
                     RNIEEATERRVIDRTQLILDIFARHARSREGQLQVELAQLNYMLPRLTGKGTAMSRLG
                     GKSGGGGAGGAGGGTGRIGVRGPGEKKLETDRRRIRDRVSKIQASIEEVRKQRALRRE
                     ARNAVPLGTIALVGYTNAGKSTLFNALSRAEVLVSSRMFATLDPTIRALRLPSNRRVL
                     VSDTVGFIRDLPKGLLTAFRATLEEVQEAALILHVSDVSNPHHDELDEEVDKILRELG
                     VDGRPRLRVLNKMDRLTPEERKVMTNGAERNAGACGPPVLVSALTGEGIEELLRRMDS
                     EMPTDPVITLSIRLPLAEGRTLAMIHALGRVLHSEIDDSHMRLDAEVPASIAKRLRLK
                     EYAVEETFPRALS"
     gene            2036..2839
                     /locus_tag="VGR03_00870"
     CDS             2036..2839
                     /locus_tag="VGR03_00870"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEV1992863.1"
                     /translation="MRKLIVTAINLLAAGMLLIGNAQAQQTPAANAQQAPAAKTGPAA
                     TPRTQPVFTLKTQKDKVSYALGANLGMNLHKQSVEVDPAIVLRGLKDALAARKMLLTD
                     EEAKAALMQLQADVRNKQMEKMKVASETNKKEGIEFLAANKAKDGVVTLPSGLQYKIL
                     TEGTGPKPTATDSVSCNYRGTLISGTEFDSSYKRGQPASFPVNGVIKGWTEALQLMPV
                     GSKWQLFIPPDLGYGDRGSGPNIGPGATLIFEVELLSIQGKDKSEVTPK"
     gene            complement(2885..3850)
                     /gene="miaA"
                     /locus_tag="VGR03_00875"
     CDS             complement(2885..3850)
                     /gene="miaA"
                     /locus_tag="VGR03_00875"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV1992864.1"
                     /translation="MSQNKALLPLVAIVGPTASGKSALGVKLAERLGGEVVACDSTQL
                     YRGFDIGTAKPSLSERRGIPHHLIDVLGPKDEATAGGYRQLALQALNDLRQRKRLPVF
                     TVGTGLYLRALLDGLADVPQRSEELRERLRASVEEHPPGHLHRVLKRLDPEAAKKIAP
                     ADEQKLIRAVEVCVLTRKPISEVHRTGRVPLEGWRVVKVGLMPPREKLNERIHTRTDG
                     MLEQGWVREVQALLESGLSETSKPFDFIGYRELRAVLRGEITLEGARGAIQRATRRYA
                     KRQLTWFRKEPGVRWFSGFGDDAGLQANVFEWLQAEGLEQQKFFG"
     gene            complement(3942..5303)
                     /locus_tag="VGR03_00880"
     CDS             complement(3942..5303)
                     /locus_tag="VGR03_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEV1992865.1"
                     /translation="MISRILVPIDARPPAAVDAPTQRRRPTTMDERTLVPAMLPIVQL
                     DGHSTIPTNLPLESIAARVVVPRDVNREAYNVREDLSTPLQPTDMDERIAVPVGAAPP
                     EMSAHPLHVPEDLVGPDIFSTGEVHLIASEQSEEKAKWQLTTRFSSIAFHILLFVALL
                     FQSKLFPPHEPTQAEMELAQRQMTVLLPPGALESLKPSTRPVQPPPRVHVDPRVLREV
                     APSITPAPAPQPERPVRELPNAPMPKPNDVQPDPRASAPTPRTDAPKPALRLEAPDTP
                     QPQHGLILPKNSGSRSIQDTIREAARSPSAGGRSGAVIGPMPRSGGALGGGGSQGSGG
                     SFGNGYEILTPTEGVDFSDYMARVVAAVRRNWYAVMPESAQLGDRGRVALQFRIMKNG
                     SVPDGEPVRLIGSGKEPLDRAAVSAIRSSNPFEPLPPAFSGPSIELRFYFLYNLPIEA
                     AYQ"
     gene            complement(5300..6298)
                     /locus_tag="VGR03_00885"
     CDS             complement(5300..6298)
                     /locus_tag="VGR03_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1992866.1"
                     /translation="MSAAAGELKRIRVAVVGTGEFGRNHARVYRELEGAELVGVFDEN
                     PERAAAIAAKYQTQVLKSLDELRGRADAASVAVPTVAHAEVGCRLLEMGLDVLVEKPM
                     AVNLGEADALLHAAKKNGRILQVGHVERFNPAVIAVEPILNRPLFFEVHRLGVFTPRS
                     LDVDVIYDLMIHDLDILLALVNEPVVEVKAVGIPVLTEKVDIAHARLEFAGGAVANIT
                     ASRVSTERVRKMRFFQQHEYISLDYARRDALRIGVKKPGPQPEFGFEKLKAPAVEPLH
                     AELEAFVDAARSRKEPRTNGAAGRAALELAGRVMASIQEHAARVQIGSFATQDKTR"
     gene            complement(6295..>6561)
                     /gene="lpxI"
                     /locus_tag="VGR03_00890"
     CDS             complement(6295..>6561)
                     /gene="lpxI"
                     /locus_tag="VGR03_00890"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEV1992867.1"
                     /translation="AGGKSLVVVKVSKPGQDMRFDVPVVGLPTIEQMRAAGATALAVD
                     AGRTLLFDRAKLIELADGAGIAIQAFPPLVVPEPNHASKGMNKE"
BASE COUNT         1349 a         2025 c         1935 g         1252 t
ORIGIN      
        1 gcaaagcgcg cgggccgatc tctgcgaggg ggctgcagtt tccacaacgt tttccacaga
       61 agagagggtc tccaatttgg aagaaaaaga gccacaagag gagccgcaag tcagtaaaaa
      121 cagaggtttt ccgcggtttt acttcacagt tagcaggtcg aaaagcgaac caagccgctc
      181 agatggcccg cggaccacga aggagccgta cggggagcat aaagatggca ctgaagagac
      241 caagaactcc gtgaacggcg attcccacga gccgaaaagg tagcgtcagc agccagacga
      301 tcggatacag cacgagagca agcagcgcga gcggccagca caagatgaac aggatgatcc
      361 acaagaagaa agcagccatg gcaacctcgt ttgttgcagc cgcaaagcgg tcagcgcgaa
      421 tggtgagcgc agagccgcca gcctcaattg tatattacga gagagcgcgg ggaaaagttt
      481 cctccacggc atactctttg agccgcagtc gcttggcgat ggacgcgggc acctccgcat
      541 caagccgcat gtgcgaatcg tcgatttccg agtggaggac gcgtcccaaa gcatgaatca
      601 tggccagcgt acgaccttcg gccagtggca accgaattga aagggttata acgggatcgg
      661 tgggcatctc ggagtccatg cgccgcagca actcctcgat cccttcgccc gtaagagcgg
      721 acacgagcac cggcgggccg caggcgccag catttctctc tgcgccgttc gtcatgactt
      781 ttcgctcttc gggagtaagc cggtccatct tattcagcac gcgcagtctg gggcggccgt
      841 cgacgccaag ttcccgcaaa atcttgtcga cttcctcgtc cagttcgtcg tggtgcggat
      901 tggagacgtc actcacgtgc aggatcaatg cggcttcctg cacttcttcg agggtcgcgc
      961 gaaacgccgt cagcaatcct ttgggcagat cgcggatgaa tccgacggtg tcggagacca
     1021 ggacacggcg gttcgaggga aggcgcagcg cgcggatcgt cggatcgagc gtcgcgaaca
     1081 tcctcgagga gaccaacacc tcggcgcggc tcagcgcgtt gaacaacgtg gacttacccg
     1141 cgttcgtgta gcccaccagc gcgatggtgc cgagaggaac ggcattgcgc gcctcgcgcc
     1201 gcagcgcccg ctgtttgcgg acttcctcaa tgctggcctg gattttgctg acgcggtcac
     1261 gaatccgacg gcggtcggtt tcgagtttct tttcaccggg gccgcgcacg ccaatgcgtc
     1321 ccgttccgcc gcccgctcca cccgcgccac cgccgccgct cttgccgccg aggcgcgaca
     1381 tcgcggttcc cttcccggtc aagcggggca gcatgtaatt caattgcgcc agttctacct
     1441 gcagctggcc ttcgcggctc cgcgcatgac gcgcgaaaat atcgagtatc agttgcgtgc
     1501 ggtcgatcac ccggcgttcc gtggcttctt caatgttgcg ctgctgcacc ggagagaggt
     1561 tgctgtcgaa gatgatcagc ggtgctttgt gtgccgtggc ttcggcgcga atctcatcca
     1621 gcttgccgcg gcccaccagc gtcgccggat ccgcggtatc gcgcatctgg aagacggttg
     1681 cagcgatctc cgctcccgcg ctacgcgcta gctcaaccaa ctctgagagc gattcgcggc
     1741 cctgctcgcc cgcaggcatg ccgggaaagc gaggcgcacg cttccatcca acgcctacaa
     1801 gcaaggcgcg ctctgctgtc gagacctgat gttgcgtttt ggtcactgag atcggcctgg
     1861 agtcatcgtg cggacttgcg catttctggc gaggctcacc cctgaatagg attatacgcc
     1921 tctctcccca gccccgcgac tcggatgcaa cccccgagga aattaatggc ctattaatgc
     1981 tcgcgcaaat ctgcggcggt ttgcgaagtc aatggacccg tgtgaaaata gggccatgcg
     2041 aaaactgatt gtcaccgcga taaatctttt ggcggccgga atgctgctga ttgggaatgc
     2101 ccaggcgcag cagacaccgg cggcgaatgc tcaacaagct ccggcggcga agacaggacc
     2161 agcggccacg ccaagaacgc agcctgtctt taccctcaaa acacagaagg acaaagtgag
     2221 ctacgctctc ggggccaacc tggggatgaa cctgcacaag cagtccgtgg aagttgaccc
     2281 ggccattgtg ttgcgtggat tgaaagacgc actcgccgcc cgcaagatgc tgttgacgga
     2341 tgaggaagca aaggccgcgc tcatgcaact gcaggccgat gtacgaaaca aacagatgga
     2401 gaaaatgaaa gtggccagcg aaacgaacaa gaaagaagga atcgagtttc tggccgcaaa
     2461 caaggccaag gacggcgtcg tcacgctgcc aagcggcctt caatacaaga ttctgacgga
     2521 gggcacggga ccaaagccca ccgcgactga ctctgtctcc tgcaactatc gaggcacact
     2581 tatctccggc acggaattcg acagctcgta caagcgcggc cagccggcgt catttccggt
     2641 aaatggagtc atcaagggct ggacggaagc attgcaactc atgcccgtgg gatcgaagtg
     2701 gcaactcttt attccgccgg atctgggcta tggtgaccgc ggctcgggcc cgaacatcgg
     2761 acccggcgcg accctcattt ttgaggtgga gctgctctcc atccagggca aggacaaatc
     2821 agaagttacg ccaaagtagc gccgtgcaag aagtgggttt gtcgctggga ctaggcaagt
     2881 cggcttagcc gaaaaacttc tgctgctcca ggccttctgc ttgaagccat tcgaatacgt
     2941 tggcttgcaa cccggcatca tcgccaaatc ctgaaaacca gcgtacgccc ggctctttgc
     3001 gaaaccaggt gagttggcgt ttggcgtagc gccgcgtggc tcgctgaatg gctccccgcg
     3061 ctccctccag agtaatctcg ccccgcagca ccgcgcgcaa ttctcggtag ccgatgaaat
     3121 caaatggctt cgaagtctcg ctcaggccac tctcaagcag cgcttgcact tcgcgcaccc
     3181 atccctgctc gagcatgcca tccgttctag tatggatgcg ttcgttgagc ttttcccgcg
     3241 gcggcatcaa tccgactttt acaacgcgcc atccttccag cgggacgcgg cctgttcgat
     3301 gcacttctga tatcggcttg cgcgtcagaa cacaaacttc gacggcgcgg atgagcttct
     3361 gctcgtccgc gggcgcgatt ttcttggccg cttccgggtc gagccgcttc aggacacgat
     3421 gcagatggcc gggcggatgc tcttcaacgc tcgcgcggag ccgctcgcgc aattcttccg
     3481 aacgctgcgg aacgtccgca agaccatcga gcagcgcgcg caaatacaat cccgtgccga
     3541 cggtgaatac cggcaggcgt ttgcgctgcc gcaagtcatt caaagcttgc aaagccaact
     3601 ggcggtagcc tccagcggtt gcctcgtcct ttggcccaag aacgtcaatc agatgatgtg
     3661 gaatgccgcg gcgttcggag aggctgggtt ttgcggtccc aatatcaaaa ccgcgataaa
     3721 gctgcgtcga atcgcaggcc accacttccc cgccgagccg ctccgcaagc ttcacaccga
     3781 gcgccgactt tcccgatgcc gtcggcccaa cgatggcaac gagaggcaag agcgctttat
     3841 tttggctcat cgaaagagga gatgccgcgc gcgaacaaaa agaaaaatga ggacaaagag
     3901 tgcgaggagg aggctgccca gaatctgttg acgcgggagt ttcactggta agcggcttcg
     3961 atcggcaaat tgtacaggaa atagaaccgc agctcgatgg acggtccgct gaaggccggc
     4021 ggcaacggtt cgaacggatt cgacgagcga atggcagaaa cggccgcgcg gtcgagcggt
     4081 tctttcccgg aaccgattag ccgcacaggc tctccgtccg gcacactgcc attcttcatg
     4141 atgcggaact gcagagccac gcgcccacgg tcgccaagct gcgccgattc cggcatcacc
     4201 gcataccagt tccgccgcac cgccgcgacg acccgcgcca tgtagtcact gaaatcgacg
     4261 ccttcagtgg gagtaagaat ctcgtagcca ttgccgaagc tgcctccgct accttgcgat
     4321 ccgcctcctc cgagtgcccc gcccgagcgc ggcatcgggc caatcactgc gccggaacgg
     4381 ccacctgcgg acgggctcct cgccgcctca cgaatcgtat cttgaatcga tcggctgccc
     4441 gaattcttcg gcaagatcag cccatgctgc ggctgtgggg tatctggcgc ttcgagcctc
     4501 agcgcgggct ttggcgcatc cgttctgggg gttggcgcgc tagcccgtgg gtccggctga
     4561 acatcgttcg gcttgggcat cggtgcgttc ggcaactctc tcaccgggcg ttccggttga
     4621 ggcgccggcg ctggagtgat cgacggcgcg acttcccgca aaacgcgtgg atcaacgtgc
     4681 actcttggag ggggttgaac aggacgagtc gacggcttta gcgattccag cgcaccggga
     4741 gggagcagca ctgtcatttg cctttgcgcg agctccattt cggcctgcgt cggctcatgc
     4801 ggtggaaata acttcgattg gaacagcagc gcgacaaaca ggagaatgtg aaacgcgatg
     4861 gaagaaaaac gcgtagtgag ttgccacttg gctttctctt ctgactgctc ggaggcgatc
     4921 agatgaactt ccccggtgct gaaaatgtcg gggccgacga ggtcttccgg aacgtgcagc
     4981 gggtgcgcgc tcatttccgg cggtgcggcg cccaccggca cggcgatacg ctcgtccatg
     5041 tccgtcggtt gcaacggggt cgaaaggtcc tccctgacgt tgtaggcttc gcgattcaca
     5101 tcgcggggca caaccacgcg cgccgcaatg gactcgagcg gcaagttcgt gggaatcgtc
     5161 gagtgcccat ccaactgcac gatgggcagc atggccggta ccagcgtgcg ctcatccatg
     5221 gtggtgggac ggcggcgctg cgttggcgca tccaccgccg cgggtggacg agcatcgatc
     5281 ggaaccagaa tccgtgaaat catcttgttt tgtcttgcgt ggcgaatgag cctatttgga
     5341 cgcgcgcggc gtgctcttgg atgcttgcca taacgcggcc tgccaattcg agcgccgcgc
     5401 gtcctgccgc cccgttggtg cgcggctcct tgcgcgagcg cgcggcatcg acgaacgctt
     5461 ccagttccgc gtgcaacggc tcgaccgcgg gtgccttcag cttctcaaat ccaaattcag
     5521 gctgcggacc gggcttcttc acgccgatgc gcagggcatc gcgccgcgca taatccagcg
     5581 aaatgtactc gtgctgctgg aagaagcgca tcttgcgcac acgttcggtg gaaacccggc
     5641 tggcggtgat attggctacc gctccacccg caaattccag ccgcgcatga gcgatgtcca
     5701 ctttttccgt caggaccgga atcccgacgg ctttcacttc caccaccggt tcgttgacca
     5761 gcgcgagcaa aatatccaga tcgtgaatca tcaaatcgta gattacgtcc acgtcgaggc
     5821 tgcgcggcgt aaacacaccc aatcggtgca cttcgaaaaa cagcggccgg ttcaagatcg
     5881 gttcaacagc aatcacggca ggattgaatc gttcgacgtg acccacctgc agaattcgtc
     5941 cgtttttctt cgccgcatgg agcagcgcgt cggcctcccc gagattcacg gccatgggct
     6001 tctcaacgag aacgtcgagt cccatttcca gaagccggca tcccacctcc gcatgcgcga
     6061 ccgtcgggac ggcgacgctc gcggcatccg cgcgtccgcg caattcatcg agactcttca
     6121 agacttgggt ctgatatttc gccgcaatgg ccgcggcgcg ttctggattc tcgtcgaaga
     6181 ctcccacgag ctccgcgcct tcaagttccc tgtacactcg cgcgtggttg cgcccgaatt
     6241 ctcctgtacc cacgacggcg acacgaatcc tcttcaactc tcccgccgcc gcgctcattc
     6301 tttattcatt cccttcgatg cgtgatttgg ttccggcacg acgagcggag ggaaagcctg
     6361 aatcgcgatt cccgctccat ccgccaattc gatcagtttt gctcggtcaa aaagaagcgt
     6421 ccgtccggcg tccaccgcga gagcggtggc acccgcagcc ctcatctgtt cgatggtggg
     6481 caaaccgacc acgggtacat caaagcgcat atcctggccc ggcttgctga ccttcacgac
     6541 aaccagggac ttgccgccgg c
//
LOCUS       DASYVO010000024         8065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_113284, whole genome shotgun sequence.
ACCESSION   DASYVO010000024 DASYVO010000000
VERSION     DASYVO010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113284"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1877
                     /locus_tag="VGR03_00895"
     CDS             <1..1877
                     /locus_tag="VGR03_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEV1992868.1"
                     /translation="LLRRIEDCRKPVVMAIHGSALGGGLELAMAGHYRLATPDAQVGQ
                     PEVKLGLIPGAGGTQRLPRLAGVAKAVEICATGDPVHALEALKEGIIDRVIEGDLLAG
                     AVAFAREVAGQPAPKTRERTGKLGTPEANAPIFAAARETARKKQRGLKAPLAAIDAVE
                     TATRLPFEEGCKIERELFRECLFSDQSKALIHVFFGEREVAKISDVPKDTPIVPVNSV
                     AIIGAGTMGSGIAMVFANVGTLVLLKEADQPALDRGLANIQKNYANSVKRGRFSQQFV
                     EERMALIKPTLSYENFERVDLVVEAVFEGMALKKEVLAQLDRVCKPGAILASNTSTLS
                     IDEIASATSRPEAVIGTHFFSPANVMRLLEIVRGKATSKQTIATCMQLAKKLGKIAVL
                     VGNCRGFVGNRMFHPYRREAQFLVEEGATVEAVDQALYDFGMAMGPLATGDLAGLDVG
                     WRIRKEYRHLEKPGVRRPLAEDRLCEMGRYGQKTGAGWYKYDDKRHAIPDPEVNKLVH
                     RWAAEAGIPQREISASEIVDRCIYGLVNEGARILEEGIALRDVDIDIIYLNGYGFPAY
                     RGGPMWYADIVGLKKVYDRICEFLEQHGELWEPAPLLKQLAEAGKTFASFNKAQSVTA
                     "
     gene            1902..3005
                     /locus_tag="VGR03_00900"
     CDS             1902..3005
                     /locus_tag="VGR03_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV1992869.1"
                     /translation="MREAAVGFRAKEDSSAVHLDTAPVRPGEELNLSALSDYLRGRIE
                     GAERGISVEQFTSGHSNLTYLLRVGDREYVLRRGPLGLVAPKAHDMSREYRVLQAIHP
                     HFREAPDVNLLCEDPAVLGAVFFLMERRKGTILRDDIPADISVVPNYAGVISEAVIQC
                     MARLHAIDIVKAGLIALGKPEGFLERQVHGWADRWNRAQTEEIPQMDEVVRWLVHRLP
                     PSPAPTMVHNDYKLDNVMLLAGSPGTIEAVLDWEMTTVGDPLADLGLTLCYWSWASAP
                     SLRARALPAVTSQPGWYTRNRFVARYAELTGRDLTHIGYYEVLGIFKLAVILQQIYYR
                     FRRGQTQDQRFCNFGDRVRGLIELAASLAEKHS"
     gene            3002..3730
                     /locus_tag="VGR03_00905"
     CDS             3002..3730
                     /locus_tag="VGR03_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1992870.1"
                     /translation="MSRIYLVRHGQAGTRESYDSLSHLGRRQSRLLGEYFVSQGIRFA
                     AAYTGAMLRQQQTAAEVSQAYARAGACFPDIQLEEAWNEFDLSRVYREIAPALAVEDA
                     EFRRGYAEMCEQMRQYAGAQDAPIHRKWQPCDTRVVDAWIGGRFPYGGETWEQFRNRI
                     AGCQRNIRSMEPQENIIIFTSAVPVAIWAGLSLDICDQRVMWLAGVLYNTSYTIVRSR
                     GEQLRLFAFNAIPHLGTPELRTRR"
     gene            complement(3765..4232)
                     /locus_tag="VGR03_00910"
     CDS             complement(3765..4232)
                     /locus_tag="VGR03_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEV1992871.1"
                     /translation="MSIPVVVPTPRSLAQLVGRELAVTDWFAVSQERIQRFAETTEDH
                     QWIHTDRERAQRESPYGTTVAHGFLTLSLLSHLIRQAVHIEGGVRLTINYGLNHVRFP
                     SPVRVGSKVRARFTLHSLREVGGALEAVYGVTVEAQDAEKPCCVAEWIVRYYL"
     gene            complement(4238..4741)
                     /locus_tag="VGR03_00915"
     CDS             complement(4238..4741)
                     /locus_tag="VGR03_00915"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV1992872.1"
                     /translation="MSPRKLNAQQAREILVKVPFNVLLDMRLHRVHQDGITIECTVRE
                     ELRNSAGIAHGGVAAALADAAVGIAIHRHVGGKRPLTTVELKINYFQPVREGRIFARS
                     HLLRIGSTLCVGRVELHDEKARPIGTAIVTYIFLDAKRGAQAKAGASKSSLARDRSRA
                     RVRIRQD"
     gene            complement(4793..6019)
                     /locus_tag="VGR03_00920"
     CDS             complement(4793..6019)
                     /locus_tag="VGR03_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV1992873.1"
                     /translation="MNFEFSDKVKDLRRRLQTFMDEYIYPNEQRFHEEVERNRWSPTR
                     IIEELKLKAREADLWNLFLPDRECGAGLTNLEYAPLCEILGRSAMAPEVFNCSAPDTG
                     NMEVLARYGTPEQKERWLKPLLAGEIRSCFAMTEPAVASSDATNIQASIVRDGNEYVL
                     NGRKWWASGGGDPRCEVAIFMGKTDPLASLYQQQSMVLVPMDAPGVKVERALTVFGYD
                     HAPHGHAEITFTNVRIPVSNILLGEGRGFEIAQGRLGPGRIHHCMRCIGVAERALETM
                     CKRVQERIAFGKTLAEQGAIRADIARSRMEIEQVRLLTFKAAYMMDTVGNKEARAEIA
                     MIKVVAPNVALRVLDRAIQAHGGAGVSQDTFLASAWANVRTLRLADGPDEVHTETVAK
                     LELRKWGKAQARSVPR"
     gene            6276..6521
                     /locus_tag="VGR03_00925"
     CDS             6276..6521
                     /locus_tag="VGR03_00925"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1992874.1"
                     /translation="MRSNHPEGNGLVERFHRSLKEEEVWLNEYRSLEEARKSISCWIS
                     EYNHDRPHRSLQNRTPREARAAFTEPQPPTKTQAPTV"
     gene            6644..>8065
                     /locus_tag="VGR03_00930"
     CDS             6644..>8065
                     /locus_tag="VGR03_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992875.1"
                     /translation="MRRLLMIVLLGLLPLGAWGQTHTFPATDTDNTFTGKNQFPHLTL
                     TLGTTPASPSTGTIRFFGNQSTGSFDCINSSGAACPFNIGAVTGTATGVFTNTQLNQP
                     FQFLSGGFSLPAFFTSISKYTDGLTVGVKVPVGSTVYQANGISGNVQLFSNNVTAGVG
                     VFGNCEADVTGGRCWGGNFQAYAGPGAPTGLGLFGIEVDVGPGSTSDSVRGILVSGAF
                     TAQPSQANGVEITPPNLSGRWKNGFICDEGALIIPGTCVAIGSSSSAANSPSQYLNFN
                     YRDSGAVLRGATIQTNITGGLDITSTNINSNLNGLGLGPNATAPFLPLTIQGPNAKAS
                     GSNFGAAFIGSAEALASNPFGLSINEVGNAADALRQIRLQTGQISLTNAGILSLQGAG
                     GRVSIGNFAPGTALDVTGAITVSDQVISTLATGTAPLAITSTTPVANLATTPTTYNAG
                     GTQITGVHIVRGTCTLGTNCNVTL"
BASE COUNT         1613 a         2431 c         2446 g         1575 t
ORIGIN      
        1 cactgctccg gcggatcgaa gactgccgca aacccgtggt catggcgatt cacgggagtg
       61 ccttgggcgg cggtttggag ctggcgatgg caggtcatta ccgcctggcc acgccggacg
      121 cgcaggttgg ccagccggag gtcaagctcg gattgattcc cggcgcgggt gggacccagc
      181 gtctcccgcg gctggcagga gtcgccaaag ccgtcgagat ctgcgccaca ggagatccgg
      241 tccatgccct cgaagcgctg aaggagggaa tcatcgatcg agtgatcgaa ggcgacttgc
      301 ttgccggcgc ggtggctttt gcccgcgagg tcgcgggcca gcccgctcct aagacgcgag
      361 agcgcaccgg caagctgggc acacccgagg cgaatgcgcc catctttgct gccgcgcgcg
      421 aaactgcgcg taagaaacag cgcggcttga aagcgccgct tgccgcaatt gacgcggtcg
      481 aaaccgccac gcggctgccg tttgaagaag gctgcaaaat cgaacgggag ctgttccggg
      541 agtgcctgtt ctccgatcaa tccaaggcgc tcatccatgt cttctttggc gagcgcgaag
      601 tagctaagat ttccgacgtc cccaaagaca ccccaatcgt cccggtgaac tccgtcgcga
      661 tcatcggcgc cggcaccatg ggcagcggaa ttgccatggt attcgccaac gtcggcaccc
      721 tggtgctgct caaggaagcg gatcagccgg cgctggatcg cgggctcgcg aatatccaga
      781 aaaactacgc gaattccgtc aagcgcggcc gcttcagcca gcagtttgtc gaggagcgca
      841 tggcgctcat caagccgact ctgagctacg aaaacttcga aagggtggac ctggtcgtcg
      901 aggccgtgtt cgagggtatg gcgctgaaga aagaggtcct cgcccaactc gaccgggtgt
      961 gtaagccggg cgcgattctg gccagcaaca catccacgct cagcatagac gagatcgcat
     1021 ccgcgacctc gcgtccggag gcggtgatcg gaacccattt tttcagtccc gccaacgtga
     1081 tgcggctgct ggagatcgta cgcggcaagg ccaccagcaa gcagacgatc gcgacctgca
     1141 tgcagcttgc gaagaaactt gggaagatcg cggtgctggt gggcaattgt cgggggttcg
     1201 tgggcaatcg catgttccat ccctatcgcc gggaagcgca gttcctggtg gaagagggcg
     1261 cgacggtcga agccgtggac caggcgctct acgatttcgg gatggccatg ggaccgctgg
     1321 ccacggggga cctcgccggt ctcgatgtcg gatggcgcat ccgcaaggaa taccgtcacc
     1381 tggagaagcc tggtgtgcgg cggccgctgg ccgaagatcg cttgtgcgaa atggggcgct
     1441 acggccaaaa gaccggcgcc ggctggtaca agtatgacga caagcggcac gcgataccgg
     1501 atccggaagt caacaagctc gtgcacagat gggcggccga ggctggcata ccgcagcgcg
     1561 agatctcggc atcggagatc gtggatcgct gcatttatgg gctggtgaac gaaggagctc
     1621 gaatcctgga ggaaggcatc gctcttcgcg atgtggatat cgacatcatt tatctgaacg
     1681 gctacggatt ccccgcttat cgcggcggac ccatgtggta tgccgacatt gtaggtttga
     1741 agaaggttta cgaccgtatc tgcgagttcc ttgagcagca cggagagtta tgggagcccg
     1801 ctcctttgct gaagcaactg gccgaagcgg gtaagacatt cgcctctttc aacaaggctc
     1861 aatccgtcac cgcgtagcgg tatccggtgg gtgttcacga aatgcgagaa gcggcagtcg
     1921 gctttcgtgc gaaggaagat tcctcagccg tgcacctcga tactgctcct gtccgtccgg
     1981 gcgaagagct gaacctttcg gctctttccg attacctgcg cgggcggatc gagggagcgg
     2041 agcgcggcat ctccgtggag caattcacca gcgggcactc gaatcttacg tacctgctgc
     2101 gcgtcggcga tcgcgagtat gtcctgaggc gaggtcccct gggccttgtt gctccaaaag
     2161 cacacgacat gtcgcgtgaa tatcgcgtac tgcaagccat acacccgcat tttcgggagg
     2221 ctccggacgt taatctcctg tgtgaagatc cagccgtcct gggcgcggtc tttttcctga
     2281 tggagcggcg gaaagggacg atcctgcgtg acgatattcc cgccgacatt tccgtggtgc
     2341 cgaactatgc gggcgtgatc agcgaagcag tcatccagtg catggctcgc ttgcacgcga
     2401 tcgacatagt aaaggccggc ttgatcgcgc tcggcaagcc ggaaggattc ctcgaacgcc
     2461 aggtgcatgg ctgggcagat cgctggaatc gcgctcaaac agaagagatc ccgcagatgg
     2521 acgaggtggt gcgctggctg gtgcaccgcc tgccgccttc acccgctcca accatggtgc
     2581 ataacgatta caagctggac aacgttatgc tcctagccgg ctctccgggc acgatcgagg
     2641 cggtgctgga ctgggagatg acaacggttg gcgacccgct ggccgacctt ggtctgaccc
     2701 tgtgttattg gtcatgggcg agcgctccat cgctgcgcgc acgcgccctg cccgcggtga
     2761 cctcgcagcc cgggtggtac acccgcaacc ggtttgtggc gcgctacgcc gaactgaccg
     2821 ggcgcgatct cactcacatt ggctactacg aggtgctggg gatctttaag ctcgcggtca
     2881 tcctgcagca gatctactac cgtttcaggc gcgggcagac gcaggaccag cggttctgca
     2941 atttcggaga tcgtgtccgg ggactgatcg aactggcagc ttcgctggcg gagaagcaca
     3001 gttgagcagg atctacctag ttcggcacgg gcaggcggga actcgcgaat catacgattc
     3061 cttatcgcat ctgggacgcc gacagtcgcg attgctcggc gaatacttcg tctcacaggg
     3121 catccggttc gcggcggcat acaccggcgc catgttgcgc cagcaacaga ccgcggctga
     3181 agtgagccag gcctacgcgc gagcgggcgc ctgcttcccc gacatccagc tggaagaggc
     3241 atggaatgag ttcgatctca gccgcgtgta tcgcgagatt gcgcctgcct tagccgtcga
     3301 agacgcggaa ttccggcgcg gatacgcgga aatgtgcgaa cagatgcggc agtatgccgg
     3361 cgcgcaggac gcccccattc accgcaaatg gcagccttgc gataccaggg tggtggacgc
     3421 gtggattggc ggacgattcc cctacggggg cgaaacctgg gaacagtttc ggaatcgtat
     3481 tgcgggatgt cagcggaaca ttcgctcgat ggagccgcag gagaacatca tcatcttcac
     3541 ctcggcggtt ccggtagcga tttgggcagg cttgtctctc gacatctgcg accagcgggt
     3601 catgtggctt gcgggcgtcc tatacaacac ctcctacacc atcgtgcgct cgagggggga
     3661 gcaattgcgc ttatttgcgt tcaacgcgat accacacctg ggtacgccgg aactgcgcac
     3721 ccgccgctga gcaacgttcc cgcgtgttct tggaatcttt gccgctagag atagtagcga
     3781 acgatccact ctgcgacgca gcagggcttc tctgcgtcct gcgcctcgac ggtgacgcca
     3841 tacacggctt ccagcgcacc gccaacttcc ctcagtgagt ggagagtgaa acgcgcgcgg
     3901 accttggaac cgacgcgcac cggcgacgga aaccggacgt gattgaggcc gtaattgatc
     3961 gtcaggcgaa ctccgccctc gatgtggacc gcctgcctaa tcagatgact caacaacgac
     4021 aacgtcagga acccgtgggc aacggtcgtt ccgtatggcg attcccgctg tgcccgctca
     4081 cgatcggtgt ggatccactg atggtcctcg gtagtttccg caaaccgctg gatgcgctcc
     4141 tggctgactg cgaaccagtc ggtgaccgca agttcgcgtc cgaccagttg cgctagagat
     4201 cgaggcgtcg gcacgacgac aggaatggac attcgtgcta gtcctgccga attcgcacgc
     4261 gggcgcgcga ccggtctctt gcgagggaag acttgctggc tcccgccttg gcctgcgcac
     4321 cacgctttgc gtccagaaag atgtaggtca caatcgcggt gccaatcggg cgtgctttct
     4381 catcatggag ctcgacacgc ccgacgcaaa gcgttgaccc gatccgtaac aggtgagagc
     4441 gggcaaagat gcgaccttcc ctaactggct gaaaatagtt gatcttcagt tcgaccgtgg
     4501 tgaggggccg tttgccgcca acatggcgat gaatcgcgat tcctaccgcc gcgtctgcca
     4561 acgctgcggc gacgcctcca tgggcgatcc cggcactgtt tcgcaattcc tcccgcacgg
     4621 tgcactcgat ggtgatccca tcttgatgca cgcggtgcag gcgcatgtcc aacagcacat
     4681 tgaagggcac tttcaccagg atctctcgtg cctgctgcgc gtttagtttt cgcgggctca
     4741 agcggctcat cgtaggagtt gtacggcaca gctatgcacg gctaggagcg gctcaacgcg
     4801 gcaccgaccg ggcctgcgcc ttgccccact tgcgaagctc cagcttggca acggtttcgg
     4861 tgtggacttc gtccgggccg tcggccaatc gaagcgtgcg cacattcgcc caggcgctcg
     4921 ccaggaaagt gtcctgggaa acgcctgcgc ctccgtgcgc ttggatcgcg cgatccagga
     4981 cgcgcagcgc gacgtttgga gccacgacct tgatcatggc gatctcggcg cgcgcttcct
     5041 tgttcccgac ggtgtccatc atgtaagccg ctttgaaggt caggaggcgg acctgctcga
     5101 tctccatgcg tgagcgcgct atgtccgcgc gtatcgcgcc ctgctcggcc agcgttttgc
     5161 cgaaggcaat acgctcctgg acgcgtttac acatcgtttc cagagctcgc tcggcgacgc
     5221 cgatgcagcg catgcaatgg tggatacgcc ccggccccag ccgcccctgt gcgatctcga
     5281 atccgcggcc ttctcccagc aatatgttcg agaccggaat gcgcacattg gtgaacgtga
     5341 tttccgcgtg cccgtgcgga gcatggtcat aaccgaacac ggtcaatgcc cgctcgacct
     5401 tcacacccgg ggcatccatg ggaaccagga ccatggactg ttgttgatac agcgaagcca
     5461 aggggtcggt cttgcccatg aagatcgcaa cctcgcagcg gggatcccct ccgcccgagg
     5521 cccaccactt gcgcccattg aggacatact cgttgccgtc acgaacgatg ctcgcctgaa
     5581 tgttggtcgc atccgacgaa gccactgcag gctcagtcat cgcaaaacaa gagcggattt
     5641 cacctgccag cagcggcttt aaccagcgtt ccttctgctc cggagtcccg tagcgggcga
     5701 gtacttccat gttaccggta tcaggggcgg aacagttgaa cacctcgggc gccatggcac
     5761 tgcgacccag gatctcgcac aagggggcat actcgaggtt tgtcagcccc gctccgcatt
     5821 cgcgatcggg caagaataga ttccagagat cagcttcgcg cgccttgagc ttgagttctt
     5881 cgatgatccg tgtcggcgac cagcggttac gttccacttc ctcatgaaag cgctgctcgt
     5941 tcgggtaaat gtactcgtcc atgaatgtct gcaagcggcg ccgcagatct ttcaccttgt
     6001 cgctgaattc gaagttcatc tccgtccgcc tatgttccgg tgaagaacct ctgccggcga
     6061 ccaccagctg gccagccatc tcggtcccga agtatctaat gcgcttgtca caattagtag
     6121 ggcattatac aaaagtcgcg tgagtcaatg tcattagcga gctactatca caatctcgct
     6181 gcccgcgcca gggagccggt caagcaggat taccgccgag gtcgctggac ggcgcgctga
     6241 gggcgggagc ttccgctgga gctacaccct gtccagtgcg ctccaatcat cccgaaggta
     6301 acgggctggt cgaacgcttc caccgcagtc tgaaggaaga ggaggtctgg ctcaacgagt
     6361 accgcagtct ggaggaagct cggaagagca tctcctgctg gatctccgag tacaatcacg
     6421 accggcccca tcgctcgctc caaaaccgga cgccgcgcga agctcgcgcg gcgttcactg
     6481 aacctcaacc accaacaaaa acccaggccc cgactgtcta gtttaggagg gtgcactaca
     6541 tcaaccttcc cttggtggat tagcccaccc aggatcagga aatccgatga tggctactat
     6601 aaaagagcat acgtagtatt cgatagtgtg aaggagtgac gcgatgagac gattgctgat
     6661 gattgtgctg ctgggtctac tcccgttggg cgcgtgggga cagacgcata cgttcccggc
     6721 gacggacacg gacaacacct tcaccgggaa gaaccagttt ccgcatctta cgctgacgtt
     6781 gggaacgact cctgcgagtc cgtccacggg aacgattcgc ttcttcggga accagtccac
     6841 ggggtcgttc gattgcatca actctagcgg ggccgcctgc ccgttcaaca ttggtgcggt
     6901 gactggaaca gctaccgggg ttttcactaa cacccaactc aatcaaccat ttcagtttct
     6961 cagcggcgga ttttccctgc ccgcgttctt cacaagcatc tccaaataca ccgacggcct
     7021 gacagttgga gtcaaggtgc ctgtcggctc aacggtctat caggcgaacg ggattagtgg
     7081 taacgtgcag ttattttcaa ataacgtcac ggctggcgtg ggggtgttcg gcaactgcga
     7141 agcagacgtt accggcggcc gttgctgggg agggaacttc caggcctatg ccgggccggg
     7201 cgcaccgacg ggattggggc ttttcggaat tgaagttgac gttggccccg gcagcacgtc
     7261 cgattcggtc aggggcatcc tcgtcagcgg agccttcacc gcccagccat ctcaggcgaa
     7321 tggagtcgaa atcactccgc caaatctttc cggcagatgg aagaacgggt ttatctgcga
     7381 cgaaggcgca ttaatcatcc ctggaacctg cgttgccatt ggttcatcgt ccagcgcggc
     7441 taactctcca tcgcaatatc tgaatttcaa ctaccgagat tctggggccg tgcttagggg
     7501 cgctacgata cagaccaaca tcacgggcgg cctggacatc acctccacga acatcaacag
     7561 caacctgaac ggcctgggac ttgggccgaa tgcgactgct ccgttcttgc cattgacgat
     7621 tcaagggccg aacgccaagg cgagtggctc gaacttcggt gcagccttca tcggctcggc
     7681 agaagcattg gctagcaacc catttggcct ttccatcaac gaggttggga atgctgccga
     7741 tgctctccgc caaatccggt tgcaaaccgg acagataagt ctcaccaacg caggcatcct
     7801 ttcgttgcaa ggcgccggtg ggcgggtcag catcgggaac ttcgccccgg gtacggcgct
     7861 ggatgtaacc ggagccatta cggtgagcga ccaagtgatt tccactctcg ctaccgggac
     7921 cgcgccgctg gccatcacct cgaccactcc ggtagcgaat ctggcgacta cgcccacgac
     7981 ctacaatgct ggggggacgc agatcaccgg cgtccacatc gtccgtggca cctgcacctt
     8041 gggaacgaac tgcaacgtga ccctc
//
LOCUS       DASYVO010000025         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_155962, whole genome shotgun sequence.
ACCESSION   DASYVO010000025 DASYVO010000000
VERSION     DASYVO010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155962"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VGR03_00935"
     CDS             <1..535
                     /locus_tag="VGR03_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV1992876.1"
                     /translation="HKWIFAPRGTGIIWARENAWAKLRPIIPSFTDEVETAWMENRAP
                     QGPTRASWVSPGGYHAFEHQWAMIEAFRFHQRIGRKRVAERIHALNEQCKEGLTKMRH
                     VKVYTPWGSTLSAGLICFDVNGMQPKAVVKRLLQHKIVASTTPYGISYARLAPSLVNT
                     PKEVDATLRAIRAMAFG"
     gene            834..1805
                     /locus_tag="VGR03_00940"
                     /pseudo
     CDS             834..1805
                     /locus_tag="VGR03_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            1832..>2059
                     /locus_tag="VGR03_00945"
     CDS             1832..>2059
                     /locus_tag="VGR03_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1992877.1"
                     /translation="MDLALRGKAAVVSGSIAGIGLAIATALAAEGAKVVVNGRTEARV
                     AAALDKIRQRVANAELRGVAGDARRGSISMAF"
BASE COUNT          464 a          567 c          642 g          386 t
ORIGIN      
        1 gcacaagtgg attttcgctc cgcgaggaac aggcatcatc tgggcacgcg agaatgcttg
       61 ggcgaaactg cgaccgatca tcccgagttt caccgatgag gttgaaacgg catggatgga
      121 gaatcgagcg ccacaagggc cgacccgggc ctcctgggtg tccccgggcg ggtaccatgc
      181 attcgagcat caatgggcca tgattgaggc atttcgcttt caccagcgga tcggccggaa
      241 gagagtcgcg gaacggatcc atgcgctgaa tgaacaatgc aaggaggggt tgacgaagat
      301 gcggcacgtc aaggtctata cgccgtgggg gagcacgcta tcggccggcc tcatctgctt
      361 tgatgtgaac ggaatgcaac ctaaggcggt agtcaaaaga ctgctccagc acaagatcgt
      421 ggcgagcact acaccgtacg ggatttccta cgcccgcctc gcaccgagcc tggtgaatac
      481 acccaaagag gtcgacgcga cgctgcgcgc aattcgtgcc atggcctttg ggtgaaggtg
      541 catctcatac ggtttccggc tgatcgactc gacatgtgag gggattcacg gggcttagcg
      601 atgcagatag gttggaacgc cacgtgtcgg ggatacacca gaataagccg aagaggaata
      661 cgctatgagc cagacctctg accctcatga tccggtaggc tttgtaataa tcgccgttct
      721 aggtgtaatg gtaatcatcg cactacacct aatatagtcc atgctggcaa aatgtagcac
      781 accattctat tggtggctat tgagccggca caggccacgc atttggaggg acagtgagta
      841 cacgcaggtt gggaaattcg gacctgaaca tcacgccggt tggatccggt gcttgggcca
      901 ttggtggcat cggctacgac tttgcttggg gcaagcagga cgacgcggac tcgattgcag
      961 caatccatcg ctcgcgtgag ctgggagtga actggatcga cactgctccc gtttacggca
     1021 cgggacattc ggaagaaata gttgccagcg ccctgaaaga gtggcgcggc tcgcgcccct
     1081 atgtttttac caagtgcgtc atgcgctggg acgaaaaagg gcgcgttacc acggttttta
     1141 atcgcgattc catccgccgc gaatgcaacg acagtttgcg ccgcctgcag gtggacgtca
     1201 tcgatctcta ttagatgcac tggccgccgg ccgacaatgg tcccggactg gaggaaactt
     1261 ggcaaacaat ggccggcctg caaaaagaag gaaaggtccg gtggatcggc gtctcgaatt
     1321 tcaccgtcgc gcagatcgag cgcgcggaga aaattgcgcc ggtgatttcg ctgcagcctc
     1381 cctattcgtt gatccaccgg cagatcgaaa cggaaacact cccgtattgc gagaagcagg
     1441 gaattggcgt catcgtgtat tcgccgatgg catcaggttt gttgaccggc gcgatgacgc
     1501 gtgagcgcgc tgcaaagctt cccgacgacg actggcggaa gcacagcccc gagttccgcg
     1561 agcccaatct ttcgaaaaat ctcaaactcg tcgagcggat gaaaaaagtt gccgcgcgct
     1621 acggacgcac tcccggcgag gtcgccatcg cttggacgct gcgattgccc gccgtcaccg
     1681 gcgccatcgt tggtgcgcgc aacgccaggc aagccgaagg cgtgatgcgt gccggcgaac
     1741 tcgagctcac cccgcaggac atcgccgaaa tcgaaggcac cgcagccgcg cacgcgggcc
     1801 gctaattctc aaaatcaatc ggagacaaga aatggatctc gcactacgag gcaaagcggc
     1861 ggttgtgtcg ggctctatag cgggaatagg gttggcaatt gccacggccc tggcggcgga
     1921 aggcgcaaaa gttgtcgtta acggccgcac ggaggcgcgt gtcgccgcgg cgctggataa
     1981 gatccgccag cgcgttgcga acgccgaatt gcgcggtgtc gccggcgacg cgaggcgagg
     2041 atcgatctcg atggccttc
//
LOCUS       DASYVO010000026         4057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_70936, whole genome shotgun sequence.
ACCESSION   DASYVO010000026 DASYVO010000000
VERSION     DASYVO010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70936"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1194)
                     /locus_tag="VGR03_00950"
     CDS             complement(124..1194)
                     /locus_tag="VGR03_00950"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEV1992878.1"
                     /translation="MKDDYLWDGSGEPDPEVQKLETVLGRYRHNQPAPAFDRVAEILP
                     EEKRSRFFNLRFSFQLAAVAAALVVAVTLFFYLRPKLVVEQGPSWEVARLEGAPKVGS
                     KAIGANSGVAKLGVGQTLVTDGSSRASITLDETGRVEVDAGSRLRLVTNGPGHKRLSL
                     ERGTIHATIWAPPGEFVVDTPSAVAVDLGCVYTLQVDDSGAGLLRTTMGWVGFKLNGR
                     ESFIPAGAVCKTRPKIGPGTPYLEDASESFRDALFRFDFEATTPVERNALLGILLVDA
                     RKNDALTLWHLLSRVSDADRPGVYDCLAALAPPPTGVTRDGILRLDRPMLDLWWNSLG
                     FGDIYLWRTYEHVWSRPPSNTK"
     gene            complement(1211..1789)
                     /locus_tag="VGR03_00955"
     CDS             complement(1211..1789)
                     /locus_tag="VGR03_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1992879.1"
                     /translation="MERQAVTVWPVTEPREEDSLVSAARNGDRAAFGRLYDRYARMVH
                     GILLARVPPHEVDDLVQEVFLKALRQLHALRDISRFGAWLGTITRNRANDYFRKSIPD
                     AKVTEPVSEDQAESRTTSHAAEQEAAVILAVVRGLPEPYREPLIFRLVEGMTGPEIAA
                     RTGLTHGSVRVNLYRGMQLLREKLAETARGAS"
     gene            2212..3789
                     /locus_tag="VGR03_00960"
     CDS             2212..3789
                     /locus_tag="VGR03_00960"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEV1992880.1"
                     /translation="MGEQVRELFNWARRRKILAAVFVAFTLAVGILIGSVVSGRVSAM
                     KSLGFSGTTATALAVPDPVPSSSSFASIVNRVEPAVVNIATTQVLERKKNPRMRRAQP
                     NEQQDDPMQDFFDRFFDGRQDGPPQAERSLGSGVIVDKRGYILTNNHVVEQATKIQVQ
                     LNGDTTRYTAKVVGVDEDTDLAVIKIDASKDLPIAKLGNSDGVQVGDWVLAIGSPFGL
                     QATVTAGIISAKDRGGIGHQFQRFLQTDAAINPGNSGGPLVDLAGQVIGINTAIITGS
                     RGYEGVGFALPSTTAINVYDQIIKQGRVTRGSIGVSFQEELSTNAITLKSLGAPYGLV
                     IEGVEPGSPAEKAGLKGGDVITSVNGQAVKTGNDLVNPIAQAPIGSKVKLNFIRDRAQ
                     KEATATVEDRTRVFPNSAGRLGDQPGEAAPPEYGLHVEELTPDRAHRVGMEGQKGVLV
                     TEVDPASFADDLGFGRGDAISVINGETVSSVADYRKAVSKLKHGEDVVFKVLRRQDSD
                     RVLTLYLSGKVPADNQQ"
     gene            3819..>4057
                     /locus_tag="VGR03_00965"
     CDS             3819..>4057
                     /locus_tag="VGR03_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992881.1"
                     /translation="MRVRKATHLALTLLVAAGFGVCAASAQAQDGAQKKSTPSKPGGS
                     SHRTKSSKRSNRRERGQKVPTPDRISEIQQALAKDG"
BASE COUNT          873 a         1141 c         1303 g          740 t
ORIGIN      
        1 cccgagcctt ggtcgttgag caaccaaagt ttactcgggt cgaggagccg acattgttat
       61 gcaatcatgt gaccctcacg gtgcatggac gggcgaagac accgtcttcg tgatggccgc
      121 gaatcacttt gtattggatg gaggccggga ccagacgtgc tcgtaggtgc gccacaggta
      181 gatatcgccg aagccgagcg agttccacca caagtccaac atcggccgat cgaggcgcaa
      241 gatgccatct cgggttactc ccgtgggcgg cggagctagc gcggccaagc agtcatagac
      301 gccggggcga tcagcatcgc ttacgcgcga gagcaggtgc cagagagtaa gagcgtcgtt
      361 cttgcgcgcg tcaacgagaa ggatgccgag cagggcgttg cgttcgaccg gcgtggtggc
      421 ttcaaagtcg aatcgaaaca aggcgtcgcg gaaggattcg gaagcatctt cgaggtacgg
      481 cgtgcccggg ccgatcttgg gccgcgtctt gcagacggct cccgcaggaa tgaaagattc
      541 gcgcccgttc agcttgaaac cgacccatcc catggtagtg cgcaggagac cggcgccgga
      601 atcgtccact tgcagggtgt acacgcagcc tagatcgacg gcgaccgcag aaggcgtatc
      661 gacgacaaat tcgccgggcg gagcccagat ggtggcgtga atcgtgccgc gctcgaggga
      721 gaggcgcttg tggccgggac cgttggtgac caggcggagg cgcgagccgg cgtccacttc
      781 gacgcggccg gtttcatcaa gtgtaatgct tgcgcgcgac gagccgtcgg tcacgagtgt
      841 ctgaccaact ccaagttttg caacgccaga gttagcgccg atggctttcg atccaacttt
      901 cggtgcgcct tccaggcgag ccacttccca ggatgggccc tgctcgacca cgagtttcgg
      961 gcgaaggtaa aaaaacagag tgaccgcaac gactagggct gcggcgacgg ccgcaagctg
     1021 gaacgaaaag cgcaaattga aaaagcggga acgcttctcc tcagggagaa tctccgcgac
     1081 ccgatcgaac gcgggcgcag gttggttgtg gcgataccgc cccaacaccg tctcgagttt
     1141 ttggacttcc gggtcaggct cccctgagcc gtcccacaag taatcgtctt tcataaacta
     1201 ttccttcgac tcatgatgcg cctcttgcag tttccgcgag tttctcgcgc agcagttgca
     1261 tcccacgata aagattgacg cgaacggaac cgtgcgtcag ccccgtgcgc gctgcgattt
     1321 ccggtcccgt catgccttcc accagtcgaa agataagagg ctcgcggtac ggttccggaa
     1381 ggccacggac cacggccaga atcacggcag cctcctgctc ggcggcgtgg ctggtcgtgc
     1441 ggctctcggc ttggtcttcc gacaccggtt cggtgacctt ggcgtcgggg atcgatttcc
     1501 ggaaataatc attggcccga tttcgcgtga tggtccccag ccaggcccca aaacgtgaaa
     1561 tatcgcgcag cgcatggagc tgtcgcaacg ccttgaggaa aacttcctga acgaggtcat
     1621 ccacttcgtg cggtgggact cgcgcgagaa gaatgccgtg gaccatgcgc gcatagcggt
     1681 cgtagaggcg cccgaaagcg gcgcgatcac cgttgcgagc ggcgctgacg agcgagtcct
     1741 cctcgcgcgg ttcagtgaca ggccagacag tcacagcctg acgttccatg cttgaggtcc
     1801 gaaagtcaat gctgtatccc acgtttaagc cattgaaagt ggatgtccct tatgggcgta
     1861 agacgcggga aatgagggaa gtgttaacag aggcgcttcc ttcctggttt ccattccata
     1921 tcttaatcgt tgaatcgcaa gtgtttacgc gaacgccaaa aggtgttttt gtcagccttg
     1981 acaacagatt aagggtttcc tgcgtccaat ggtgaggaga acaagaatcg gaaggcaccc
     2041 cccggccagt aggacagtta ttccttcgcc gaactgcttg ccagcagcac caccgccgga
     2101 gtagcggtgt tatactttta aggttggccc cggccggatt tggggcaggc cgaagagcgt
     2161 tcaaccgaga cgaagagggc tgtgcgggcc tggactagag gagtaatcga catgggagag
     2221 caggtaagag agctttttaa ttgggcgcgc cgccgcaaga tcctcgcggc ggtatttgtc
     2281 gccttcacgc tggccgtcgg cattctgatc gggtcggtcg tttccggacg cgtctcggcg
     2341 atgaagagcc ttggattttc gggcacaacg gcgacggcct tggcggtgcc agacccggtg
     2401 ccctcgtcca gttcctttgc ctcgatcgtc aatcgcgtcg agcccgcggt ggtgaacatc
     2461 gccacgacgc aagtgctgga gcgcaagaag aatccgagga tgcgccgcgc gcagccgaac
     2521 gagcagcagg atgatccgat gcaggatttc ttcgaccgct tctttgatgg ccggcaggac
     2581 gggccgccgc aggccgagcg cagcctgggt tcgggcgtca tcgtcgacaa gcgcgggtac
     2641 atcctgacga acaatcacgt ggtcgaacaa gcgacgaaaa tccaagtgca attgaacggg
     2701 gatactacca ggtacaccgc caaggtcgtc ggtgtggatg aggatacgga cctggcagtg
     2761 atcaagattg atgcgagtaa ggatctaccc atcgcgaagc tgggaaattc cgacggcgtg
     2821 caggttggcg actgggtact ggcgatcggc agccccttcg gactgcaggc cacggtgacc
     2881 gcgggaatca tcagtgcgaa ggaccgcggg gggattggac atcagttcca gagatttctg
     2941 caaaccgatg ccgcgatcaa cccggggaat tcgggcgggc cgctagtgga cctggccggc
     3001 caggtgatcg ggatcaatac ggcgatcatc acgggcagcc gcggctatga aggtgtcggt
     3061 tttgcactgc cctcaaccac ggcgatcaac gtttacgacc agatcatcaa gcaaggccgc
     3121 gtgacgcggg ggtccatcgg cgtcagcttc caggaagagt tgagcacgaa cgcgatcacg
     3181 ctgaagtcgc tgggcgctcc ttacggtctg gtgattgaag gtgtcgagcc cggaagtccg
     3241 gcggagaagg ccggtttgaa gggcggcgac gtgatcacca gcgtgaacgg tcaggcggtg
     3301 aaaaccggga atgatctggt gaacccgatt gcgcaagcgc ccatcgggag caaggtaaag
     3361 ctgaacttta ttcgtgaccg cgcgcaaaaa gaggcaaccg cgacggtcga agatcgcacg
     3421 cgggtattcc cgaattccgc gggccgcctg ggcgatcagc ccggcgaagc agcgccgccg
     3481 gaatacggct tgcacgtcga agagctaacc cccgaccgcg cgcaccgcgt cggaatggaa
     3541 gggcaaaagg gcgtgctcgt aaccgaagtc gatccggctt cctttgccga tgatttggga
     3601 ttcggacgcg gagacgcgat ttcggtgatc aatggcgaaa cggtgagttc cgtcgccgat
     3661 taccgcaagg ccgtgtcaaa actaaagcat ggcgaggatg tcgtgttcaa ggttcttcgc
     3721 cggcaggaca gcgaccgggt cctgacactc tatttgtcgg gcaaagtgcc ggcggacaat
     3781 cagcaatagg gatttagcga cagccaatac aaacacccat gcgagtgcga aaagcgaccc
     3841 acctggccct gacgctgctc gtggcagcgg ggttcggtgt gtgtgcggct tcggcgcaag
     3901 cgcaggacgg ggcgcaaaaa aaatccacac cttccaagcc gggcggctct tcgcaccgga
     3961 cgaagtcgtc gaaaagatca aaccggcggg agcgcgggca aaaagtcccc actcccgacc
     4021 gcatttccga gattcagcag gcgctcgcga aagacgg
//
LOCUS       DASYVO010000027         4328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_269105, whole genome shotgun sequence.
ACCESSION   DASYVO010000027 DASYVO010000000
VERSION     DASYVO010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269105"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VGR03_00970"
     CDS             complement(<1..654)
                     /locus_tag="VGR03_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992882.1"
                     /translation="MAASEPNGAPRWRLIVVGGHTRSIGKTQLVCDVISAFPQAKWIA
                     GKITQYGHGVCAQNGENCDCAPDEHVCAINWEMQADTGTDSARFLAAGARRSFWLRTK
                     QGFLAEGLPLLRAALQQSIEAGGSELPPVIVESNSLLQFLKPSLYFAVVDPAKEDFKD
                     SARAALDRADALVLRGSSDTFPATGPSWIKLPALLLKEKASVTQQEGEPLPEPLHVLV
                     "
     gene            complement(641..1663)
                     /gene="lpxD"
                     /locus_tag="VGR03_00975"
     CDS             complement(641..1663)
                     /gene="lpxD"
                     /locus_tag="VGR03_00975"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:TIGR01853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEV1992883.1"
                     /translation="MMRRTAKELAEAIGARLEGDGAAELGGVAAPERAGAHDLIYVES
                     AKHAPRAAASAAICVVAAEGIALAGKTVLRSAQPKVAFARAAELLLGRAPIASGIHPT
                     AIVAPLARVAPDAGIGPYAVIGEDAHVGAGTQIGAHSVIGAGCWIGESCRIHPRVTLY
                     AGVRVGSRVEIHSGAVIGADGFGYAFGEGKYWKFPQAGIVEIGDDVEIGANTTIDRGS
                     LDDTRIGEGVKLDNLVQVGHNCQIGAHTVIAAQVGLSGSCVFGKRVVVGGQAGFGERC
                     KLEDGSVIGGQSGVLGGKTIRSGETVWGTPARSLERFKELFAWYGRLPDLAARIKELE
                     ARLGSK"
     gene            complement(1660..2577)
                     /locus_tag="VGR03_00980"
     CDS             complement(1660..2577)
                     /locus_tag="VGR03_00980"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV1992884.1"
                     /translation="MKEWIEYAAVWAIVKGLGVLPRSLARSLAAGVVRVFYALLPRLR
                     KIAEVNLCIAFPEWSDAQRKTVVRGMLRNLGWMAAEFARFPKYSKENIEQIVVLDGHE
                     NFLEGQRRGKGVLYLTGHIGAWELSSFAHALYGFPLHYMARRIDNPRIDVLVNGYRCL
                     SGNHPIFKNESARVTLRVLKEAGTVGILADQNTMPQEAAFVDFFGKMASTTTGIARVA
                     LHTDAAVVPGYAIWDKGRRKYRLRFEPPVELIRTGDTERDVLENTQKFTRVIEEIIRK
                     YPEQWVWVHGRWNTRPKGEPPVYDFSRGR"
     gene            2673..>4328
                     /locus_tag="VGR03_00985"
     CDS             2673..>4328
                     /locus_tag="VGR03_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEV1992885.1"
                     /translation="MTETTASIAENPRPSTSARNGWAVLILATLYVCYFSHLGAIGFV
                     GPDEPRYAWIARDMVETGDWVTPRLYGKPWFEKPVLYYWGAALCFKLFGVSEAAARLP
                     SAISALLATLALAWFALRLYGAETARWLLLLLPTTVGMIGFSHAAATDMPFSGMLTIA
                     MVCAAVVIGLTRDANSPVVPQTPWLALILFGFFLGLAVLAKGPAAIILSGGAIFFWAL
                     FTKRWRDAFRLFHPAAIASLCLTALPWYILCARRNPDFFRIFIIEHNFKRYLTPEFQH
                     IQPFWFYIPITILALLPWATLAPLLFADAWRAWSENRWRSSPALLFTCWAVLTLVFFS
                     ISKSKLPGYVLPAIPPIVLLLTRAASLRLRSAGPGSRWIGIVSRMTIFCIAGATLAEI
                     DKIPFQVDYSPPGPLGIVIVTLGFSGIIASILGAFKKTVPALSLSVAVFLGAIVFSLT
                     GDAVWSLDKGISSRVAASYAWETTRNSPDRQILVRGLPRAERYGLSFYLHRELQEWTP
                     NERQHGWVLTNYEGQEGLRQLGLTCPPMRRGAPIAICEEDGLAD"
BASE COUNT          814 a         1507 c         1078 g          929 t
ORIGIN      
        1 aacgagcacg tgcagcggct caggcaacgg ctccccctcc tgctgtgtaa cggatgcctt
       61 ttccttcagc aaaagtgcgg gaagcttgat ccaggaaggc ccagttgcag gaaatgtgtc
      121 cgaagatccg cggagcacca aggcatcggc gcggtccagc gcggcgcggg ccgaatcctt
      181 gaaatcttcc ttggccggat cgaccactgc gaaatacaaa ctgggcttga ggaattgcag
      241 caaggaattg ctctccacaa tcaccggcgg cagttccgac ccgccagctt cgatcgactg
      301 ttgcagcgcc gccctgagaa gcggcaggcc ctcggcgaga aatccttgtt ttgtcctcag
      361 ccagaacgat cgccgtgccc cggccgccag gaaacgggcg ctgtctgtcc ccgtgtccgc
      421 ctgcatctcc cagttgatgg cgcaaacatg ctcatcgggt gcgcaatcgc aattttctcc
      481 attctgcgcg cagactccgt ggccatactg cgtgatcttg cccgcaatcc atttggcctg
      541 gggaaacgcg gagatcacat cgcacaccag ttgcgtcttg ccaatgctgc gcgtgtgtcc
      601 accgaccaca atcaagcgcc agcgcggcgc cccgtttggt tcacttgctg ccaagtcttg
      661 cctcgagttc tttgatgcgc gcggccagat ccggcagccg gccataccac gcgaacagct
      721 ctttgaatct ttccagcgat cgagccggtg ttccccaaac tgtctcgccg ctgcgaatgg
      781 tttttcctcc gagcacgccg ctttgcccgc cgatcaccga cccgtcctcc agtttgcagc
      841 gctcgccaaa gcctgcctgc ccgcctacaa ccacgcgttt tccaaataca cacgacccgg
      901 acaatcccac ctgagccgca atcaccgtgt gcgccccaat ctgacagttg tggcccacct
      961 gcaccagatt gtccagcttc acgccttccc caatgcgcgt gtcgtcgagc gagccgcgat
     1021 cgatcgtggt attcgctccg atttccacgt catcgccaat ctccacaatc cccgcctggg
     1081 gaaatttcca gtacttccct tcaccgaacg cataaccgaa accgtccgcc ccaatcaccg
     1141 cgcctgaatg aatctccacg cggctcccca cccgcacgcc tgcgtacagt gtgacccgcg
     1201 ggtgaattcg gcaactctca ccgatccagc aacccgcacc gatcaccgag tgtgctccaa
     1261 tctgcgtgcc ggcgccgaca tgcgcatctt ctccaatcac cgcgtacggc ccgatacccg
     1321 catccggcgc tacgcgcgcg agcggcgcga caatcgccgt cggatgaatc cccgaagcaa
     1381 tcggggcgcg cccaagcagc agttcggctg cccgcgcgaa cgccaccttg ggttgcgcac
     1441 tccgcaatac cgtcttgccc gctaacgcga ttccctcagc cgcaacaaca cagatcgcag
     1501 ccgaagccgc ggcccgcggc gcatgcttcg cggattccac atagatcaag tcgtgcgctc
     1561 cggcgcgctc cggggcggca actccgccaa gttcggctgc gccatcgcct tccagcctag
     1621 cgccaatcgc ttccgccaat tccttagcag ttcgtctcat catcgacctc gcgaaaaatc
     1681 gtataccggc ggctcacctt ttggccgggt attccaccgc ccgtgcaccc aaacccactg
     1741 ttcgggatac ttccggataa tttcttcaat cactcgcgta aatttttgcg tattctccag
     1801 aacgtctcgc tccgtatcgc ccgttcgaat caactccacg ggcggctcga agcgcaaccg
     1861 gtattttcgg cggcctttat cccaaatggc atatcccggc acgaccgccg cgtccgtatg
     1921 cagcgccacg cgcgcaatcc cggttgtggt gctggccatc ttcccgaaaa aatccacgaa
     1981 cgccgcttct tgcggcatgg tgttctgatc cgccagaatc ccgaccgttc cggcctcttt
     2041 caaaaccctt agcgtcacgc gcgccgattc gtttttgaag atcggatgat tccccgacag
     2101 gcaccggtac ccgttcacga gcacatcgat tctcggattg tcgattcgcc gagccatgta
     2161 gtgcagagga aatccgtaca gcgcgtgcgc aaaagaggac aactcccacg cgccgatgtg
     2221 ccccgtcaaa tagagcacgc ctttcccgcg ccgctgccct tccagaaaat tttcatggcc
     2281 atcgagcacc acaatttgct cgatattctc tttcgaatat ttcggaaacc gcgcaaactc
     2341 cgctgccatc cagcccagat tccgcaacat cccacgcaca accgtcttgc gctgcgcgtc
     2401 gctccactcc gggaacgcaa tgcacaaatt cacttcagcg atttttctca atcgcggcaa
     2461 caacgcatag aaaacgcgca ccactccagc agcaagactc ctcgccagcg aacgcggcaa
     2521 cacacccagc cccttcacaa tggcccacac ggccgcgtat tcgatccatt ccttcatggc
     2581 ctacccaagt tcgctcgtgt ttcccgccgg gcgacagtat gcaagaggtc cgtcgctgga
     2641 agatagtcgt tcgatgttag actctgcttt ccgtgaccga aacaaccgct tccatcgccg
     2701 agaacccgcg ccccagcacc tccgcccgga atggctgggc cgtcctcatc cttgcgacgc
     2761 tctacgtctg ctacttcagc catctcggcg ccatcggctt cgtcgggccg gacgagccgc
     2821 gctacgcctg gatcgcgcgc gacatggtcg aaaccggcga ctgggtcacg ccgcgccttt
     2881 acggtaagcc ctggttcgaa aaaccggttc tttattattg gggcgcagcg ctttgcttca
     2941 aactcttcgg cgtcagcgaa gctgccgcgc ggctgcccag cgccatctcc gcgttactcg
     3001 ccacacttgc gctagcctgg ttcgcactgc gtctctacgg cgcggaaacc gctcgttggt
     3061 tgctgcttct gcttccgacc accgtcggca tgatcggttt ttctcacgcc gccgccacgg
     3121 acatgccctt cagcggcatg ctaaccattg ccatggtctg cgccgcagtc gtcatcggct
     3181 taacccgcga cgcaaactct ccggtggtac cgcaaactcc gtggctcgcc ctcattctct
     3241 tcggtttctt cctcggcctc gccgtcctcg ccaaaggccc cgccgccatc attctctccg
     3301 gtggcgccat atttttctgg gcgctcttca ccaagcgctg gcgcgacgcc ttccgccttt
     3361 ttcatcccgc cgccatcgcg tccctctgcc tcaccgctct accctggtac atcctctgcg
     3421 cccgccgcaa ccccgatttc ttccgtatct tcatcatcga gcacaatttc aagcgctacc
     3481 tcacccccga attccagcac atccagccct tttggtttta tatccccatc acgatcttgg
     3541 cgcttttgcc ttgggcaacc ttggccccgt tgttatttgc ggacgcatgg cgagcttgga
     3601 gtgagaaccg atggcgcagc tctccggccc tcctgttcac ctgctgggcc gttctcactc
     3661 tagtcttctt cagcatctcg aaatccaagt tgccgggata tgttttgcca gccatcccgc
     3721 cgattgtttt gctgctcaca cgcgccgcct cacttcgctt gcgcagtgcc ggccccggca
     3781 gccgctggat cggcatcgtc agcagaatga caattttctg tattgcaggc gcgacgcttg
     3841 cagagatcga caagatccca tttcaggttg attactcccc tccaggaccg ctcggcattg
     3901 tcattgtgac gcttggcttc agtgggatta ttgcttcaat tttgggagcg ttcaagaaga
     3961 cggttccggc gctctcactc agcgtggccg ttttcctcgg agcgattgtt ttttccctga
     4021 ctggagacgc cgtatggtcg ttggacaaag gaatctcctc ccgcgtggct gccagctacg
     4081 cttgggagac cactagaaat tcgccagata gacaaattct tgtacgcggg ctgccgcgtg
     4141 ccgagcgcta cgggttgagc ttctatctac atcgcgaact gcaggaatgg acgcctaatg
     4201 aacgacagca tgggtgggtg ctcacaaact atgaaggcca agagggactg aggcaacttg
     4261 gtctgacctg ccctccgatg agacgtggcg cgccgatagc aatttgcgaa gaagatggcc
     4321 tagcggac
//
LOCUS       DASYVO010000028         6199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_43226, whole genome shotgun sequence.
ACCESSION   DASYVO010000028 DASYVO010000000
VERSION     DASYVO010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43226"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VGR03_00990"
     CDS             complement(<1..632)
                     /locus_tag="VGR03_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEV1992886.1"
                     /translation="MDRRLRNRILIFLALALIAAFVLVRLSGRQPVAKVAVVTPAREN
                     LAASISSNGKVEPISPSVMRAQIATFVEKVYVLEGQSVKKGQRIMDLDVKDAASQLAD
                     ARSKLLRAQEDLRAARAGGRSDDAARVAGDLAKARAQRDRLARENEALQRLFAQQAAT
                     RDELAANEVELSRAQAEFDKTSAAKHEFDRQAKLDAERTALQVDQLKNAAA"
     gene            complement(602..757)
                     /locus_tag="VGR03_00995"
     CDS             complement(602..757)
                     /locus_tag="VGR03_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992887.1"
                     /translation="MSRFGPGGIDDAGQAIGMPVQRERIATDYGWNKSPVAAMRLRWI
                     EGSEIES"
     gene            1188..1937
                     /locus_tag="VGR03_01000"
     CDS             1188..1937
                     /locus_tag="VGR03_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1992888.1"
                     /translation="MIEARGLCKRFQDKKRGEIRAVENVSFGCQPGKIYGLLGANGAG
                     KTTTLRMLATILEPTDGTAIVAGYDIIEHPDKVRANVGFLSTATALYPRLTAQELVEY
                     FGRLNGLDEATLKKRVDTIFDRLEMNDFRDRRCDKLSTGMKQKTSIARTLVHDPPVMI
                     FDEPTIGLDVMTARTIVTFIRECRDHGKTVIFSTHVMSEVEKLCDSIGIIQDGKLLAE
                     GTLAELRGKYGQTDLEEIFVRVVGPYHKAEA"
     gene            1943..3151
                     /locus_tag="VGR03_01005"
     CDS             1943..3151
                     /locus_tag="VGR03_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1992889.1"
                     /translation="MNVRNIGLVYRKELTEWLRDRRTLISTVVVPLLAFPLLMTGISA
                     LAIAVIGKAEKEKPTVVLLGGEDSPRILEDLRRLEKIELVPTTPNWKDRIANKEIRAA
                     IELPAGFQASLEGAEPETVKIYYYEGDLKSSFGANHVEKYFKDYRDKIIKERLSAKHL
                     PATLLKPFEVKQENVAPPEKVSGAAFGGLISYMVILLCMTGATYPAIDLTAGEKERGT
                     METILSSPIARLDLVLGKFFLVLSAALATATLSVISMGLSFGVLGKFRALGSSSQGRG
                     LELHIGLKTALAVFAMALPLAVFFAGVLMTIALFAKTYKEAQSYLMPMTFAVVIPAVA
                     SLLPGVELTPKLALIPILNTSLVCKEIVAGTYHWNMIALIFVSSCVYAAAGLFLAVKM
                     FQRESVLFRS"
     gene            3772..4830
                     /locus_tag="VGR03_01010"
     CDS             3772..4830
                     /locus_tag="VGR03_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1992890.1"
                     /translation="MADAQLISVRALSKHFRTFQRREGILGGFVNLFHRDYQTLKAVD
                     NIQFEIARGEMVGYIGPNGAGKSTTIKMLTGILVPTSGEILSNGFVPWRQRAAYVKTI
                     GVVFGQRTQLWWDIAVIESFKLLRRIYGVSQRDFDERMELFHQVLALGDFLNTPVRKL
                     SLGQRMRCDLAAALLHNPPLLFLDEPTIGLDVVAKDHIRQFLRAINQRYKTTILLTTH
                     DLDDIEELCRRILIIDRGVLLYDGPLGALKERLLRTKQIKFILRDGEQAARLASFEAS
                     LAASRLDGSLHLERLDEMTCRIVFDRKQVSSSDLIRQILAAVEVRDFVIEDEPIEEIV
                     KRIYGGEVFSNLALPGEG"
     gene            4827..5675
                     /locus_tag="VGR03_01015"
     CDS             4827..5675
                     /locus_tag="VGR03_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992891.1"
                     /translation="MTTAPQLPASSPAVFWPALAAYREFARIGFINILAFRLRYYTGI
                     LTYLINVTVYYFIWRAVFSHADLSIAGFALPQILTYVSVGWILRSFYWNTIDQEMAYE
                     VIEGKIAMDLIKPVSVQWMWLSRATGESAFRLLLLTAPTAVVIMLLFPVQRPASGLHF
                     GLFALSAAGSFLLMGAINFMIGTCAIPLKSILALIRAKFWLIELLSGLLIPISFFPRR
                     LQIVSSWLPFEHIAFTPLQIYLGKLSIAASLQRMALEFAWVAALLFLGHLWWFRATRK
                     ITIHGG"
     gene            5677..>6199
                     /locus_tag="VGR03_01020"
     CDS             5677..>6199
                     /locus_tag="VGR03_01020"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV1992892.1"
                     /translation="MGRLISGLRRHAMLLGAYFAQYAKVRVSYRGDFFVSLATSMAAT
                     AFSLGFVWLIFQRVPRLANWRLEEVVFLYGFSLIPFGLFNVLSINLYEFGATYIMEGK
                     FDRVLLRPISSLFQVLFETFRIESFHEVLAGLLIVVWASLRLGHHWTVLDVVLLVFFG
                     LCGGVIYISVFLFL"
BASE COUNT         1160 a         2018 c         1704 g         1317 t
ORIGIN      
        1 gccgcagcgt tcttgagttg atccacctga agggccgtgc gctcagcgtc cagtttcgcc
       61 tgccggtcga attcgtgctt cgccgcggag gtcttgtcga actccgcctg cgctcgggac
      121 agctcgactt cgttggccgc caattcgtcc ctcgtggcgg cctgctgggc gaaaagccgc
      181 tgcaacgcct cgttttcccg cgccagccgg tcgcgctgcg cccgtgcctt ggccaagtct
      241 ccggccaccc gtgcggcgtc atccgagcgc ccgccggcgc gcgcggcccg cagatcttcc
      301 tgcgcgcgca ggagtttcga tcgcgcatcg gcgagttgcg acgcagcatc cttcacatcc
      361 aggtccatga tgcgttggcc ctttttcacg gactgccctt cgaggacgta gaccttctcg
      421 acgaacgtcg cgatctgcgc gcgcatcacg gagggagaga tcggctcaac cttgccgttg
      481 ctgctgatgg aggcggccag gttttcccgc gccggagtga cgaccgcgac tttggctacc
      541 ggctggcgcc cgctcaggcg aaccagtaca aaggcggcga tgagcgccaa cgcgaggaag
      601 atcaggattc gatttcggag ccttcgatcc atcgtagtct catcgcggcc accgggctct
      661 tattccaccc gtaatccgtg gcaatccgct ccctctgaac aggcatgccg atcgcctgcc
      721 cggcgtcgtc gattcctccc gggccgaagc ggctcattct agcaagcctg cacccgcttt
      781 ctccagcagc ctcgcgccgc gggcttcttc cgcagcttac atccgcttgg ttgcgcaacc
      841 gcggggcgcg gccccccgcg ggccatcgaa atctttgtgt tacaattcgc gtatgagcgg
      901 cgaagccccc ccgacgacac agaagcccga agccaccaaa gcggggcatg ggccggcgct
      961 accgcctccg ccgggcgccc cggcgctgca tcgcgtatgc atccaatgca acaacatgtt
     1021 tcgcgtctcg ccggaaaact tcgacgcgaa acaatgcccc aactgccaca agggctaggg
     1081 acttggacgc gggcgacttc cccttgtaat attgcacagg cgtcgtgcgt agtactgtgc
     1141 gaaacggccg gtgggtggac tcaaagcaat ttcagcagcg gggttccatg attgaagccc
     1201 gaggtttgtg caaacggttc caggacaaga agcgtggaga gatccgcgcc gtggaaaacg
     1261 taagcttcgg ttgccagccg ggcaagatct acggcctgct cggcgccaac ggggccggca
     1321 agaccacgac gctgcgcatg ctcgccacca tcctcgagcc gaccgatggc acggcgatcg
     1381 tcgccgggta cgacattatc gagcacccgg acaaagtccg cgcaaacgta ggattcctct
     1441 ccacggccac ggcgctctat ccgcggctga cggcgcagga attggtcgag tatttcggcc
     1501 ggctgaacgg tctggacgaa gcgaccttga agaaacgcgt ggacacgatc ttcgaccgac
     1561 tggagatgaa tgattttcgc gaccggcgct gcgacaagct ttccacaggc atgaagcaga
     1621 agacctccat cgcgcgcacg ctcgttcatg atccgcccgt catgattttc gacgagccca
     1681 ccatcggtct cgacgtcatg accgcgcgga ccatcgtcac ttttatccgc gaatgccgcg
     1741 accacggcaa gacggtcatc ttctccaccc acgtcatgag cgaggtcgag aagctgtgcg
     1801 attcgatcgg catcatccag gatgggaagc tgctcgccga aggcacgctg gcggagttgc
     1861 gcggaaaata cggccaaacg gaccttgaag aaattttcgt cagggtggtc ggcccctacc
     1921 acaaggcgga ggcgtaattc agatgaacgt gcgaaacatc ggattggttt atcgcaagga
     1981 actcaccgag tggctgcgcg accgccgtac gctcatctcc accgtcgtgg tgcccttgct
     2041 cgcgtttccc ttgctgatga caggcatcag cgccctggcc atagcggtga tcggaaaagc
     2101 ggaaaaagag aagcccaccg tggtcctcct cggcggagaa gattcgccca gaatcttgga
     2161 agacctgcgg aggttggaaa aaatcgaact ggtgcccaca actcccaact ggaaggaccg
     2221 catcgccaac aaggagatcc gggccgcgat cgaattgccg gcggggttcc aggcttcgct
     2281 cgaaggggcc gaaccggaaa ccgtcaagat ttactactac gaaggagact tgaaatcctc
     2341 cttcggggca aaccacgtgg agaagtattt caaggattat cgcgacaaga tcatcaaaga
     2401 gagattgtcg gccaaacatc tgcctgcgac gctcctgaag ccgttcgagg tgaaacagga
     2461 aaacgtcgcg ccgccggaaa aagtcagcgg cgcggccttc ggcggtttga tcagttacat
     2521 ggtcatcctg ctgtgcatga ccggcgccac ctacccggcg attgacctca ccgcagggga
     2581 aaaagagcgc ggcacgatgg agacgattct ttcaagcccg attgcgcggc tggacctcgt
     2641 cctcggaaag ttcttcctgg tactttccgc ggcgctggcc acggcaacac tctccgtcat
     2701 ctccatgggg ttgtccttcg gcgtgctggg caagttcagg gcgctcggct cttcttcgca
     2761 gggccgaggc ctggagctgc acatcggcct gaaaacggcc ctcgctgttt ttgccatggc
     2821 cttgcctttg gctgtgtttt ttgccggagt gctcatgacc atcgcgctct ttgccaagac
     2881 ctacaaagag gcacagagct atctcatgcc catgaccttc gccgtggtca ttcccgccgt
     2941 ggcctcgctg ctccccggcg tggagctcac gcccaagctg gccctcatcc ccattctcaa
     3001 caccagcctg gtgtgcaagg agattgtggc cggcacctac cactggaaca tgatcgcgct
     3061 gatttttgtc tcgagctgcg tctatgccgc ggcggggctg tttcttgcgg tgaagatgtt
     3121 ccagcgcgaa tccgtcctct tccggtcctg aaacgaaggg ccaaaaacaa aaccgtcccg
     3181 tttttaacag ctaaagagca gaattgtgtg cgcgagcctt ggagtatctg ttcaaaagca
     3241 agaacgcaca gagggacatg gcaccatagg cgatcatgta cgcattgctt gcgtgttgat
     3301 aagtttggtc caaaacttca tcggacttct tgctcgggac aaggtgtatt agccccggca
     3361 aaatttgaaa gctccccaat aggaaaatga ggctgatagc tttccgaaac gtctcctctc
     3421 ttggcaaatc acgcgcaatt ttagagctca agaagcacgt cgcaatccca ataacagcga
     3481 tcggtatgcc aaacagagca tctccgtcgg cgtgaattcc gaagcctgcc accatactcg
     3541 cgactagaat ccataaacct agcattagga ggagccctgc gctcacatac cgcacaaatt
     3601 gcgtggagaa cctcacaatt ggatgatacg cgcgggttct taattcacaa tttgcgatag
     3661 caaaagccca tacgtaagtg tcaacccggc aggatcgtgc gaaggggccg gagacgcagg
     3721 ccctgatacc aacgcggggt ttgcgcgtcg aagtgctaga attgttgttc catggctgac
     3781 gcgcagctca tctccgtccg agcgctttcc aagcatttcc gcaccttcca gcgtcgcgaa
     3841 ggcatcctcg gtggtttcgt caacctcttc caccgcgatt accagaccct gaaggccgtg
     3901 gacaacatcc agttcgagat tgcccgcggc gagatggtcg gctacatcgg cccgaacggc
     3961 gcgggaaaat ccaccaccat caagatgctc accgggatcc tggttccgac ctccggtgag
     4021 attctttcga acggcttcgt gccctggcgc cagcgcgccg cctacgtgaa gaccattggc
     4081 gtggttttcg gccagcgcac gcagttatgg tgggacatcg cggtgatcga atcgttcaag
     4141 ctgctgcggc gcatctacgg agtctcccag cgcgatttcg acgagcgcat ggagctcttc
     4201 catcaggtcc tcgccctcgg cgatttcctg aacacgccgg tgcgcaagct atcgctcggc
     4261 cagcgcatgc gctgcgacct ggccgcggcg ctgctccaca atccgccgct tctgttcctc
     4321 gacgagccca ccatcgggct cgacgtcgtc gccaaggacc acattcgcca gtttctccgc
     4381 gctatcaacc agcgctacaa gaccaccatt ctgctcacca cccatgacct cgatgacatt
     4441 gaagagctct gccgccgcat cctgatcatt gaccgcggcg tcctcctcta cgacggcccc
     4501 ctcggcgcgc tgaaggagcg gctcctgcgc accaagcaga tcaagtttat cttgcgcgac
     4561 ggcgaacagg ccgcgcggct cgcctccttc gaggcctcgc tcgccgccag ccgcctcgac
     4621 ggctcgctcc atctcgagcg gctcgatgaa atgacttgcc gcatcgtctt cgaccgcaag
     4681 caggtttcct cttcggacct tatccggcag atcctcgccg ccgtggaagt ccgcgatttc
     4741 gtcatcgaag acgaacccat cgaagaaatc gtcaagcgca tctatggagg cgaggtcttc
     4801 tccaatctcg ccttgcccgg ggaaggatga ccaccgcgcc gcagcttccg gcctcctcgc
     4861 ccgccgtttt ttggccggcc ctcgcagcct accgcgaatt tgcgcgcatc ggcttcatca
     4921 atatccttgc cttccgcctg cgctattaca ccggcatcct gacgtatctc atcaacgtca
     4981 ccgtctacta cttcatctgg cgcgccgtct tttcccacgc ggacctgtcc atcgcgggct
     5041 tcgcgcttcc ccaaatcctg acctatgtaa gcgtgggatg gattctgcgt tccttctact
     5101 ggaacaccat tgaccaggaa atggcctacg aagtcatcga aggcaagatc gccatggacc
     5161 tgatcaagcc cgtgtcggtg cagtggatgt ggctctcccg cgcgacgggc gagtccgcct
     5221 ttcgcctcct cctgctgacc gcgccgacgg ccgtggtcat catgctgctt ttccccgtgc
     5281 agcgccccgc ctccggcctg cacttcgggc tcttcgctct ttccgccgcc gggagctttc
     5341 tcctgatggg cgcaatcaat ttcatgatcg gcacttgcgc catcccgctc aagtccatcc
     5401 tcgcgctcat ccgggcgaaa ttctggctca tcgagctgct ttccgggctc ctcatcccca
     5461 tcagtttctt cccccgccgg ttgcagatcg tttcttcctg gctccccttc gagcacatcg
     5521 cctttacgcc gctgcaaatc tatcttggca agctgtccat tgccgcctcg ctccagcgga
     5581 tggcgctcga gttcgcctgg gttgcggcgc tgctcttcct gggtcatctt tggtggttcc
     5641 gcgccacgcg gaaaatcacc attcacgggg gttgaaatgg ggcggctgat ttccggattg
     5701 cgtcgccacg ccatgcttct cggcgcctac ttcgcgcagt acgccaaagt gcgtgtcagc
     5761 taccgcggcg atttcttcgt ttcccttgcc acgtccatgg ccgcgaccgc gttttccctt
     5821 ggctttgtat ggctcatttt ccagagggtg ccgcgcctgg ccaattggcg cctggaagag
     5881 gttgtctttc tttacggctt ctccctcatc cccttcggct tgttcaacgt cctctcgatc
     5941 aatctttatg agttcggcgc cacctacatc atggaaggca agttcgaccg cgtcctcctc
     6001 cgcccgatct cctccctctt ccaggtgctc ttcgagacct tccgcatcga atcctttcat
     6061 gaagtactcg ccggcctgct catcgtcgtg tgggcctcgc ttcgcctcgg tcaccactgg
     6121 acggttctcg acgtcgtgct tctcgtgttt ttcggcctct gcggcggtgt catctacatc
     6181 tcggtatttc tcttcctca
//
LOCUS       DASYVO010000029        20671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_269162, whole genome shotgun sequence.
ACCESSION   DASYVO010000029 DASYVO010000000
VERSION     DASYVO010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 20671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20671
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269162"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VGR03_01025"
     CDS             <1..371
                     /locus_tag="VGR03_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992893.1"
                     /translation="VVNLENDNAVSEDPFFLVKVIEKVNSQWLHALPDFGNTLAAHDE
                     EYAYRGIEGMFGHAYNISHVKETEVGEKGKIAHVDLAKTFGIAKRHGYKGYFSMEWDS
                     AGDPYAGTAGLIEKTLRNLS"
     gene            complement(523..3180)
                     /locus_tag="VGR03_01030"
     CDS             complement(523..3180)
                     /locus_tag="VGR03_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1992894.1"
                     /translation="MNWFKQLFSRCRLYDDLSEEIQEHLEEKIEELVASGMSRKEAAA
                     AARREFGNVTMIEEDSRNVWRWPSIENLLMDIHYGLRMLGKNPGFSTLAVLTLALGIG
                     ANTAIFSVIDSVLLRPLPYHDPDSLVMVWESNSQHPNPHNTVSPPNFLDWQSRNTVFS
                     SMAYIFDERDNLTGNGDPEEVVVQDVSTNFFSLLGVNPILGPGFIPENGQPGHDNVVI
                     LGYGLWKERFAGDHAIVGKSVLLNGHPQTVVGVAPQNFQWFIKDGSLTGAKPQMWSPF
                     VFPQSFHDHKQIGRFITVVARLDPGASHSQAQSQMNAIASRLEQEYPDFDGHWGVNVV
                     PLRQQITGDLRPALLVLFGAVAFVLLIACANVSSLLLARAASREREMAIRTAIGASRW
                     RIARQLLMESLLLSLIGGGIGVALAVWGTNALLAASPRNLLDLSSVSMDLRVLTFAIA
                     ATFVAGLLFGFLPSYISAHSRISETLKEGGRGSSSANRRAFARNAFVVAQLGLALVLL
                     TGSGLLIRSFVRLIGVDPGFDTGHLFTFKVSLPRSKYGTDQLRLAFFQQLLARLAAVP
                     GVRSASMESFPPLAGLGAATGVHLLSQPQQSLMDLPVANVRVVGPDYFDTMNIPLRAG
                     RLFNAQELTVEKHVTIINQAFADRYLHGVNPLGQKAAIYMKSLVENQLQPSEIIGVVG
                     DVHQMGLDTAPEPTVYWPHPELVYSGMTILVRTSNDPFALASAARTELQQLDPELPLA
                     GVATMDQLLADSLSRSRFTMLLLGIFSAVALVLAAVGIYGLIAYSVTQRTQELGIRIA
                     LGAQRRDVLRLVLAQGTRLTLLGVAVGVLAALAFSRLLATLLFGVSATDPLTFAGVAG
                     LLAFVALLACFIPARRATRVDPIVALRYE"
     gene            complement(3177..3518)
                     /locus_tag="VGR03_01035"
     CDS             complement(3177..3518)
                     /locus_tag="VGR03_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1992895.1"
                     /translation="MKKSEQRDLFPGALEMMILRTLKRQPLHGYALVQHIKRTSNDLL
                     QIEEGSLYPALQRLLREELVKAEWAVSSTNRRVRIYKLTPAGAKHLQRQVSSFEKMLE
                     GITRVLAPGES"
     gene            3720..4334
                     /locus_tag="VGR03_01040"
     CDS             3720..4334
                     /locus_tag="VGR03_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEV1992896.1"
                     /translation="MALFGDGGRRELEKKAKAEGRLPPGQSLTLKWPVLHCGSVPHFE
                     AGKWDFKISGLVEEPVRLGWEEFRALPQTEVTSDFHCVTRWSRLDNRWKGVLFTDVMK
                     LVKRKPGAEFALLLAEAGYTANVPLADLQRPDVLFTFEHDGEPLTAEHGGPLRLIVPH
                     LYAWKSVKWVRGFTLLDHDRLGFWERNGYHAYGDPWKEQRYSGS"
     gene            4676..5182
                     /locus_tag="VGR03_01045"
     CDS             4676..5182
                     /locus_tag="VGR03_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992897.1"
                     /translation="MATLQSKSIYHSAFVRVVLLGLMALCGASTPLLGQETAGGKFTL
                     TENTRFGKKFLPAGAYTFSIEPTGTLQSVNSIGGGGQPVLVIVRGERKPGPIAVIFAM
                     ASRTSHALNSSKLVIAPVDNGMAVSSMYLDQPGLVLDFQRWNRKDKTQMLAQAAPPEP
                     ASASKATD"
     gene            5309..6043
                     /locus_tag="VGR03_01050"
     CDS             5309..6043
                     /locus_tag="VGR03_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEV1992898.1"
                     /translation="MNGDSGSLAVKNLELDMLHEIGNRIASADPLPEVLGRAVEVISA
                     VVESDSCFVYVLEGNELVLRASKNPHSEVVDRLKLQVGQGITGWVAKHRRPVAVARDA
                     FQDPRFRSFNELPEDLYQAFLSVPVLSRDKLVGVINLQHRQPHNYSQREIHLISTIGF
                     LVGAEIEMARLEGENSELSEQLETRKVIERAKGILQRDLQLSEQEAYVTLQRQSRQRR
                     KSMKEIAEAIVLSEDVKRSPGKPRPS"
     gene            complement(6148..6609)
                     /locus_tag="VGR03_01055"
     CDS             complement(6148..6609)
                     /locus_tag="VGR03_01055"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEV1992899.1"
                     /translation="MWSKQQTVAEPPAVSPSQGSAASVVPFNSASNSRLGSTPARNSA
                     RLGSSLHIKGQVTGAEDLQIDGKVDGPIALRGHELTVGPTAQLNSEIHAGEVIVSGKI
                     VGNIHARGRVEIKKDGSIVGDISSARISIEDGAHFKGRIEIDPAKSQAAAD"
     gene            complement(6772..7185)
                     /locus_tag="VGR03_01060"
     CDS             complement(6772..7185)
                     /locus_tag="VGR03_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992900.1"
                     /translation="MNQHTYLRAYLAGIAVPTVFLLVIMSGYCVLRYVYDFPVPVERV
                     IVFPMAIVPNLWGLWNILFIALRSRWRLSIGLHGALLPILLAPLGLVVASLLNFPAFA
                     FASRVWPIATPVALIVYYFAWKYIVGFLNRVQEIA"
     gene            complement(7200..7433)
                     /locus_tag="VGR03_01065"
     CDS             complement(7200..7433)
                     /locus_tag="VGR03_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992901.1"
                     /translation="MTNKENNKEIEALLKAAVAPVKDAELRRDLWPQMLRKLDEQPAR
                     VDKVPWFDWALAAILSAVLFLFPGAIPALLYHL"
     gene            complement(7430..7957)
                     /locus_tag="VGR03_01070"
     CDS             complement(7430..7957)
                     /locus_tag="VGR03_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV1992902.1"
                     /translation="MELLERFAAGDLEAFEALFRERQKEVYAWIVRIVRDHGIAEDLT
                     VETFWRIYRSRLRFRPAGNFRAWARRIATNAAFDHLRTARRETELPDDLASAGVADPA
                     VRRETRDRIKQAFFQLPAKYRLVATLALIEEEPYNKIAEAMGISTALVKMRVFRAVRI
                     LRKKLHSLGAEVGAK"
     gene            complement(8136..11345)
                     /locus_tag="VGR03_01075"
     CDS             complement(8136..11345)
                     /locus_tag="VGR03_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEV1992903.1"
                     /translation="MSKELVISAASHERRVAILEEGQLVEIYIEREKEFALVGSIYKG
                     KVTRVLPGMQSAFVDIGLDGDAFLYVSDVFENLEDYDHGHPHEQPQASVASLPPASSS
                     PVEMLPGESLARAGDHHDEAHHQEFHQDETHPEDEQHAAHEEAHATNGEHLEEHHDVV
                     PSPPMEDQHAEAGEERQPDSNASAPQNFAPRYNPTQNYPARSGSDRPGQNFGRGGDRG
                     SDRGGDRGGDRGRGGRWGRRGGRPRGGRPPGAPGGRNLPPSKYASPQGGESRGYDNRG
                     GEPRSYDNRRPEAPRSPAPPSSSVTEEEIILPGESLAKYRGKPAVAPAPAPTVEHETH
                     EEQSTVEEITSRTTGNLPATAPSGSSVPRRFSGGLPRWLLADAGAEPEAPSATAGQAA
                     ETPQAPAEQESAPVRSDADLSEDQVAALAADFAEAKHEEATEEAEADAIAGGAEFDEE
                     ETEEQTEEGTEEAEETPAAEVSGLSEEEVEEVEAGRAANRAEFAADVAQEHAEDDATH
                     STHEHGEHEHIEDEHAGTEHVEEEVISAAPGESVAEGTPAIEDDVILLPGETRSPRVG
                     GPAAPREDFPRDSARIGGNPRSRFQRPFRSGGRDRGRDSRGGRPDSRGGRPDGRGGRP
                     DSRGGRPDSGGRPISGGPRFERRPSGPRQDRGYQGGRSGPSRRPQLISDMLKAGQDVV
                     IQIAKEPLGKKGARITSHVALPGRFLVYMPTVHHTGVSRKIISAENRSRLRRLVSESG
                     GAYPGGFIVRTAAGGATDDEIRTDIEFLGKSWNEIKERSEQRKAPSLLHRDLNLVERI
                     LRDYVSDDFTGIWIDNEEEYTKIVEFVSRFQPKLVNMVKLYTKETPIFEEFGIQHELD
                     KALRAKVWLKSGGYIVINHTEALVAIDVNTGKFVGKGSTRLEDTIVKTNLEAVKEIVR
                     QIRLRDLGGIIVVDFIDMEERRNREKVLSALQQALEEDKAPSKALSFNEFGLVCITRK
                     RTKQALERVLCQPCPYCTGSGMVKSIPTLCYEIQSEARKMAAADHEGPNLTIRVNPEI
                     AKALKTRESMLIDELEQTAHKHIIIQSDATLHWEQYDIY"
     gene            complement(11767..12879)
                     /gene="rodA"
                     /locus_tag="VGR03_01080"
     CDS             complement(11767..12879)
                     /gene="rodA"
                     /locus_tag="VGR03_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEV1992904.1"
                     /translation="MKDAPGTRDYDWWLLAILAAICTLGVMEIYSATHGSSLTGMHTK
                     QIRWLVIGFLLMFVLSRLDYHLILDQAPILYLIGLAALVAVLLVGHTHFGAKRWIAIP
                     ALGELVQVSELVKLIIIIVLARFFAEVRSDQLDLRDLIKAGLLVGGPLVLILKQPDLG
                     TALVLIPLLVVGAFLAGLQWQHAAVFSLAGILLVGAVFYPPVSKRILKPYQRDRITSF
                     LHPEEDAKGSGYQLLQSKIAVGSGGFWGKGFRNGSQNQLGYIPVRYSDFIMSAWAEEQ
                     GFKGVLLALGLYMALLLRLVQNAQRAKDRAGMFLVMGVAAALGFHVLVNVAMVIGAMP
                     VTGIPLPLMSYGGSATLFVFLAIGLVMNVRLRRFVN"
     gene            complement(12992..14926)
                     /gene="mrdA"
                     /locus_tag="VGR03_01085"
     CDS             complement(12992..14926)
                     /gene="mrdA"
                     /locus_tag="VGR03_01085"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEV1992905.1"
                     /translation="MSYWFRNDNRLPQGRLAVASYIIVGMIGVLLLGFWKLQVIDADK
                     YSSMAERNRVRYIPVIAPRGRMLDRDGRVLVDNRPAFSVLLLRDDPALVEKHLPGISD
                     GLGIPLDDLRDQLTNTKNLPKFQPIIIKPDASPADIAFIESHRSDLPVLEMISVSRRR
                     YLPGGFLAHAAGYVGEVSEQQIEASNGKLRPGDFAGKTGLERQYNDSLQGTDGMRRVV
                     VNSIGKEVERLSTQEAIPGKQIQLTIDYDLQQIAEQSLGPRPGAVVALDPHTGEVLAM
                     VSHPALDPNEFAVRISGDDWKSLNEDPLHPLFNRAIQAQLAPGSVFKIVTATAMLEDK
                     NPPESFTTFCPGYATFYGRQFKCWVYGKSSHGVVGFHKAILQSCDIFFYNVGMRLGID
                     RLSYYGSKLGLGHKTGIDLPSEEPGLMPSAEWAERVFHRKWYAGETISVSIGQGAVTT
                     TPLQLARTIGGIAMGGVFKQPHLLKDAPNVGEERFALSEPTVEKITDGMYGVVNDEGG
                     NAKGVRLPGIEVSGKSGTAQVIGYATRDRMGKQKKFEDNAWFVGYAPRRNPEIVVSVL
                     VQESGKHGGEAAGPVVKEVIKAYYDKKTKKTQGQVTAENKRSDSGSGSATAAAIAPRA
                     ALKPEDNAVVPARASAEPQR"
     gene            15307..15975
                     /locus_tag="VGR03_01090"
     CDS             15307..15975
                     /locus_tag="VGR03_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992906.1"
                     /translation="MTAETNREKPLRLALIGMSGAGKTFWAKKLAASRYPAVSCDDRI
                     EQKLAPRLAAGGFAGINGVAAWMGWPNSATYTERESEYLAEEIHTLDEILTELEKQPE
                     KSLVLDTTGSVIHTGNNLLMRLRRQMTIVHLAASAEERQLLIERYLNDPKPVLWRGAF
                     QPRAGEKPRETVARCYPALIAARRQSYEALAHCTLQVAALRDGSLDAPAFLKMIQDKT
                     GSPR"
     gene            15986..17287
                     /gene="thrC"
                     /locus_tag="VGR03_01095"
     CDS             15986..17287
                     /gene="thrC"
                     /locus_tag="VGR03_01095"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV1992907.1"
                     /translation="MRYRSTSRKAPLTSLRGAVLRGLAPDGGLYMPVEIARHSPEELE
                     EFRGLPFTEVCFRVVRPFATPDAPEEVLWQIVSEAINFPVNLVSLSPGLHILELFHGP
                     TLAFKDFGARFMARLMGYFVRGETRTLTVLVATSGDTGSAVAHGFLQVPGIRVVILYP
                     SKRISEAQEKQFTTLGENITALEVAGTFDDCQRLVKQAFSDAELNKHTWLTSANSINI
                     GRLLPQMFYHVAAYRQLPVASVPLIVSVPSGNFGNLTAGIFAKRIGLPVAKYVASTNA
                     NDVVPEYLRTGEFHPRSAQATYSNAMDVGNPNNFPRLLDLCRNRLEYVQKEIWGHGAT
                     DEETLAAMKMLHDKFGYIADPHTAVGVLGWEAYKSQHPEPAQGLVLATAHPAKFADVV
                     MKAIGTAPPLPDRLAAYLKREKLSLPISSSYDEFKQFLLTR"
     gene            complement(17340..17867)
                     /gene="mreD"
                     /locus_tag="VGR03_01100"
     CDS             complement(17340..17867)
                     /gene="mreD"
                     /locus_tag="VGR03_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV1992908.1"
                     /translation="MNDAFDIRGESHIEVHKFRAGAIIIATLLALVIQASFPIHFAKV
                     AVLDLPLLVTIYFGLSRRNPSTGLLLGMFIGLLQDSLSGPTVPLGLYGIAKTIIGYLA
                     SSIGARLDTEHPAARFALTAAFYVAHQGLMVLTRRILLAQLEPWFNMHLFVAALVNAL
                     VGVFLFLLLDRLRKN"
     gene            complement(17864..18766)
                     /gene="mreC"
                     /locus_tag="VGR03_01105"
     CDS             complement(17864..18766)
                     /gene="mreC"
                     /locus_tag="VGR03_01105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV1992909.1"
                     /translation="MVAIPSRHKSLVLLAGVIVLQVLMLAVQIKRPSQGRLIRVWTVG
                     AVSPFERAGSHGFGWIHDTWRHYFALQNTTKENEQLRHENDALKLQISQLQGKAAEAD
                     RIAVLLNFRQSHADVPMIAARVIATGAGTASLTIQLDRGERDGIRKNMGVITPDGVVG
                     KVVETYPNASQVLLLTDKESGVGAMLADSRIQSPVGGQGEPLLVMKYVPNDDTVNLGE
                     RVITSGMDRIFPRDLPVGTVAEIKAGNPFKQIRIKPAANLERLEEVLVLLSLHPLEMK
                     NEENTRDALPSAPGAANPPPSAKR"
     gene            complement(18878..19918)
                     /locus_tag="VGR03_01110"
     CDS             complement(18878..19918)
                     /locus_tag="VGR03_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEV1992910.1"
                     /translation="MTKNGYNLRSVFSLFSSDLAIDLGTANTLVFAHGKGIVVNEPSI
                     VAINKSTGEVVAVGREAKEMLGRTPGNVVAIKPMKDGVIADFKVTEKMLTYFIQKAHN
                     RRVLVHPRIVIGVPSEITPVEKRAVQDSAYRARASEVFLVEQAMAAAIGAGLPIEEPS
                     GNMVVDIGGGTTDIAVISMSGIVYSRSVRVAGNEMDEAVMQYLKRKYNLLVGERTAEQ
                     IKMEIGSAYPLEKPLTMEVKGRNLIEGVPRTVTIDDSEIREALSECVATILNAVRVAL
                     ERTPPELSADISDRGIVLTGGGALLKNLDKRIREETGLPVSIADDPLASVVLGTGKML
                     SDFKLLRQVCID"
     gene            complement(20058..>20671)
                     /locus_tag="VGR03_01115"
     CDS             complement(20058..>20671)
                     /locus_tag="VGR03_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992911.1"
                     /translation="LAPWEKITIPSGGPIQKSNVLAPPELVEPLNLAPIIAENPKISP
                     VRFEWKPVQDAVSYTLRISTTAMFTKTVKDAKVGGTAVEITGLDPGDYFWNVTATDGK
                     KQTSEVSEIFKFTLVAQGKSQAMLLEIDGTQLHGHVAEIVGRTEPGAALIVNGQPVPN
                     VSPDGAFRHFTESLDPGQHTISIIGSNRRGGTAIKQVSIVVPK"
BASE COUNT         4322 a         5821 c         6389 g         4139 t
ORIGIN      
        1 tggttgtaaa cctggagaac gacaatgcgg tcagtgaaga tccgttcttc ctggtgaaag
       61 tgatcgaaaa agtgaacagc cagtggctgc acgcgctgcc ggatttcggg aatacgctgg
      121 cggcgcacga cgaggagtac gcttacaggg gaatcgaagg gatgtttggc cacgcgtaca
      181 atatctctca cgtgaaggaa acggaagttg gcgagaaggg caagatcgcg cacgtggatt
      241 tggcgaagac gtttggaatc gcgaaacggc acggatacaa aggatatttt tcgatggaat
      301 gggacagcgc gggcgatccg tatgcgggga cggcggggct cattgaaaag acactaagaa
      361 atctctccta agaattgatc tcacaaattg gttcgtagcg atggcaagag cagtcttcag
      421 gcgctgaaag ccacgtgcga caaccgtaga accatttcct cagcggctga agccaaattc
      481 agagtgctcg ccttatgtcg gagctaaaag ctccgccccc cgtcattcgt accggagcgc
      541 gacgatggga tcgacgcgag tggcgcggcg cgcggggatg aagcacgcca gcagagcgac
      601 aaacgcgagt aagccggcga cgccggcaaa ggtgagcggg tcggtggcgc tgacgccgaa
      661 aagcagggta gccaagaggc gcgagaaagc gagcgcggcg agaacgccga cggccacgcc
      721 caggagcgta aggcgtgtcc cctgtgcaag cacgaggcgc agaacatcgc ggcgctgggc
      781 gccgagagcg atgcgaatgc cgagttcctg ggtccgctgg gtgacgctgt aggcgatcaa
      841 gccgtagata ccgacggcgg caagcaccag agcgacagcg gaaaagatgc cgagaagaag
      901 catggtgaag cgcgagcggg aaagagaatc ggcgagaagc tggtccatgg ttgcgacgcc
      961 ggccaagggc agttccggat ccaattgctg aagttcagtg cgcgccgccg atgccagggc
     1021 gaaaggatcg ttggaggtac gcacgagaat cgtcatgcct gaatagacga gctcggggtg
     1081 cggccaatag acagtgggtt cgggtgctgt gtcgagtccc atctggtgga cgtcaccaac
     1141 cacaccgatg atttccgacg gctggagctg attctcgaca agacttttca tatagatggc
     1201 ggctttctgg ccgaggggat tgaccccgtg gagatatctg tccgcgaaag cctgattgat
     1261 gatggtcacg tgcttttcta ccgtcagttc ctgcgcgttg aaaagacggc cggcacgcaa
     1321 aggaatattc atggtgtcga aatagtccgg cccgacaacg cggacgttgg cgacgggaag
     1381 atccatgagc gattgctgcg gctggctgag gagatggacg ccggtagcgg cacctaggcc
     1441 ggcgagcgga ggaaagcttt ccatgctggc ggagcggacg ccgggaaccg cggcgagtct
     1501 ggcgagaagc tgctggaaga aagccaagcg cagctggtcc gtaccgtact tggagcgtgg
     1561 aagagagacc ttgaaggtga agagatgacc ggtatcaaat cctggatcaa cgccgatcaa
     1621 gcggacgaag ctgcgaatga gcaagccgga gccggtgagc aggacgaggg ccaggcccaa
     1681 ctgggctacc acgaaggcgt tgcgcgcgaa ggcgcggcgg tttgcggaag aggagccgcg
     1741 gcctccttcc ttgagggttt cggagatgcg cgaatgtgcc gagatgtagg aaggcagaaa
     1801 gccgaagagc aaaccggcga caaacgttgc ggcgatggca aaggtgagaa cacggagatc
     1861 catcgagacg gaactgaggt cgaggagatt cctggggctg gcggccaaaa gagcgttggt
     1921 gccccaaaca gcgagcgcga caccgattcc gccgccgatc aacgagagaa gcaagctttc
     1981 catcagcaac tggcgcgcga ttcgccaacg gctcgcgccg atggcggtgc gaatggccat
     2041 ttcgcgctcg cgactcgccg cgcgggcaag aagcaggctt gaaacatttg cgcaagcaat
     2101 cagcaagaca aaagcaaccg cgccgaagag gactagcaaa gcgggacgca gatcgccggt
     2161 gatctgctgg cgcagaggaa cgacgttgac gccccagtgc ccgtcgaaat cgggatactc
     2221 ctgctcgagc cgggaggcga tggcgttcat ctgggactgc gcctgggaat ggctggcgcc
     2281 gggatcgagg cgcgcgacca cggttatgaa gcggccgatc tgcttgtgat cgtgaaagga
     2341 ctgcggaaac acaaagggag accacatttg cggttttgcg ccggtgagcg agccgtcctt
     2401 gatgaaccat tggaaattct ggggcgcaac gccgaccacg gtctgagggt gtccgttcag
     2461 caggacagat ttgccgacga tggcgtgatc gccggcgaag cgttctttcc aaaggccgta
     2521 gccgagaatg acgacgttgt cgtggccggg ctggccgttt tcgggaatga agcctgggcc
     2581 gaggatggga ttgactccga gcagagagaa gaaattggtg gagacgtcct ggacgacgac
     2641 ttcctcgggg tcgccatttc cggtgagatt gtcgcgctcg tcaaaaatat aagccatgct
     2701 ggagaagacg gtattccggc tctgccaatc gagaaaattc ggcggagaga cggtgttgtg
     2761 ggggttgggg tgctgggaat tgctttccca gaccatcacc aaactgtccg ggtcgtgata
     2821 cgggagaggg cggaggagga cagaatcaat caccgagaaa atcgcggtat tcgcgccaat
     2881 gccgagggca agagtgagga cggcgagggt ggagaatccg ggattcttgc ccagcatgcg
     2941 aagcccatag tggatatcca tgagaaggtt ctcaatggac ggccaccgcc agacgttgcg
     3001 gctatcctct tcaatcatag ttacattgcc aaattcgcgg cgagcggcgg cggcagcttc
     3061 ctttctggac atgccactgg caacgagttc ctcgatcttt tcttcgaggt gctcctgaat
     3121 ctcttcggaa agatcgtcgt acaggcgaca acgagaaaag agctgcttga accagttcat
     3181 gattcacccg gcgccaggac gcgggtaatg ccttcgagca ttttttcaaa gctggagacc
     3241 tgccgttgga ggtgcttggc gcctgccggc gtcaacttgt aaatgcgaac gcggcggttg
     3301 gtggaggaaa cggcccattc tgccttgacc aactcttccc taagaaggcg ctgaagagcg
     3361 gggtaaagcg agccttcctc gatttgaagc aagtcgttgg aggtacgctt gatgtgctgc
     3421 acgagggcgt agccgtgcag gggctggcgc ttcaacgttc ggagaatcat catctccaag
     3481 gcgccgggaa aaaggtctcg ctgctcggat ttcttcatag cggggagtct gctccagacc
     3541 ttgggtcaga gaccatttcg gacaaggcct agagtaaaat agtataagag aagttggcag
     3601 tcaagagctt ttttcaaggc gagttagaag atgcgattga atttgtgccg tctagccggc
     3661 tctactagag tgggaaactc ttgagtaggc tcagtatcta attttgtggc gggatcgtga
     3721 tggcactgtt tggggatggc gggcggagag agttggaaaa gaaggcgaag gcggaggggc
     3781 ggctgccgcc ggggcagtcg ctgacgctga agtggccggt gctgcattgc ggcagcgtgc
     3841 cgcattttga agcggggaaa tgggacttca agatttccgg gttggtagaa gagccggtgc
     3901 ggctggggtg ggaggagttt cgcgcgctgc cgcagacgga agtgacgtcg gattttcact
     3961 gcgtgacgcg ctggagccgg ttggataacc gttggaaggg cgtgctgttt accgatgtga
     4021 tgaagctggt gaagaggaag cccggtgcgg agtttgcgct gctgctggcg gaagcaggct
     4081 acacggcgaa tgtgccgctg gcggacttgc agcggccgga tgtgctgttt acgttcgaac
     4141 atgatggtga gccattgacg gcggaacatg gcgggccctt gcgactgatt gtgccgcatc
     4201 tctacgcgtg gaagagcgtg aaatgggtgc gcgggttcac gctgctggac cacgaccggc
     4261 tgggattctg ggagcgcaac ggatatcacg cgtacggcga tccatggaaa gagcagcggt
     4321 actcgggaag ttagtggttt gagattcgag gttcgagaaa aacccgagct gacgatgcgt
     4381 ccagatcgta ctccgcatca ggaggccgca tcgattgacc gcgtgcatca tggcgatgaa
     4441 atctttcgat tggactcgcc gggaatattc tgcaaaagtg aagttgtaga gttcgcgcgt
     4501 ttgccatgcc gcaacgtacg acgagaactt cgtagttcct cagcaagccc tgtccagcct
     4561 gtcgtttaaa tcggttgttc tcagacctcg tggtctgaaa cgaacacaag gacaacgcag
     4621 ttgacgctcc tagcacagct tggacgtcca tcccaatcta aagtggagga acgaaatggc
     4681 aacgcttcaa agtaaatcga tttatcacag cgcctttgtg agagtggtac tgctggggct
     4741 aatggccctg tgcggagcct caactcccct tctcggccag gagacggccg gtggaaagtt
     4801 cacgctgacc gaaaatacgc gcttcgggaa gaaatttctt ccggcaggag cctacacatt
     4861 ttccatcgaa cccacgggta ctttgcaatc cgtcaactcc atcgggggtg ggggccagcc
     4921 ggtgctagtc atcgtgcgcg gggagagaaa gcccggcccg attgccgtca ttttcgcaat
     4981 ggcgtcgcga acctcccatg ctctcaactc aagtaagctg gttatagcgc cggtggacaa
     5041 tggaatggca gtgagctcca tgtacctgga ccagccggga cttgttctgg atttccagcg
     5101 ctggaaccgg aaggacaaga cgcagatgct tgctcaggcg gcacctcctg aacctgcttc
     5161 ggcatccaag gcaaccgatt gaggagagtt tcccgacccg tgctggcggg ccgttcaagc
     5221 ggcccgccag cagcgggcgt ttttccttcg attcagcgat tgcaaaattt cgattggaca
     5281 ctgctatccc gcctcggtaa attaggttat gaacggcgat agcggctctt tagcggtcaa
     5341 gaaccttgaa ttggacatgc tgcatgagat tggcaaccgc atcgcttctg ccgatccatt
     5401 gcccgaagtc ttgggacgcg ccgtagaagt catttccgcg gtggtggagt ccgattcgtg
     5461 ttttgtctac gtcctggagg ggaatgagct agtcctgcga gcctccaaaa atccgcattc
     5521 cgaagttgta gatcgattga aactccaggt aggacaaggc atcaccggtt gggttgccaa
     5581 gcataggaga ccggtggccg tcgcgcgcga tgctttccag gatccacggt tccgatcgtt
     5641 caacgaactg cccgaggatc tctaccaagc attcctgtcc gtgcctgtgc tatcccgaga
     5701 caagcttgtg ggagtcataa acctgcaaca ccggcagccg cacaactaca gccagcgcga
     5761 gatccatctg atttccacca ttggatttct ggtgggcgcc gagattgaaa tggctcggct
     5821 cgagggagag aactcagaac tttccgaaca attggagacc cgcaaggtca tcgagcgcgc
     5881 caaaggaatc ctgcagcggg atttgcagct gagcgaacaa gaggcctacg tcacgttgca
     5941 acggcaaagc cgccaacggc gcaaatcgat gaaagaaatc gcggaagcga tcgtcctgag
     6001 cgaggacgtc aaacgtagcc cgggcaagcc aagaccaagt tagatccgct gcgccgttct
     6061 gtcatcccat tagaaagccg ggaagacaga ctttgcgccg ggtagagcgg aagtccattg
     6121 cagcgaggat gagagaacca ggccgtacta atcggccgcg gcttgggact tggccgggtc
     6181 gatttcgatg cggcccttga agtgggcgcc gtcctcgatg ctgatgcgcg ccgaggaaat
     6241 atctccgacg atcgagccat ctttcttgat ttcgacacgg ccgcgggcgt ggatgttgcc
     6301 gacaattttg ccggagacga tgacttcgcc ggcgtgaatt tcggaattga gctgggcagt
     6361 ggggccaacg gttagttcgt ggccgcggag cgcgatgggg ccgtccactt tgccatcgat
     6421 ctgcaggtct tcagcgccgg tgacctgccc tttgatgtgc aggctggaac ccagacgcgc
     6481 cgaattacgg gccggggtgc tgccgaggcg cgagttggac gcagaattga atggaacgac
     6541 tgaagctgcc gaaccttgag agggcgaaac agccggcggt tcagccacag tttgttgctt
     6601 agaccacata aaacctccct tttccgattc ccaagaatgc ggattgctcc gggtgggagg
     6661 agcagcgtgc attcactcta gccgcgccag gcaagaagaa gcaacgacga aaaggtattg
     6721 gattgcgcag atccaaaact gaccgtgcgg acaggtcagg cgctccgagg ctcaagcgat
     6781 ctcttggact cgattgagga agccgacgat gtatttccaa gcgaagtagt agacgatcag
     6841 cgcgacgggg gtggcgatgg gccagacgcg cgaggcaaaa gcgaaggcgg gaaaattcag
     6901 aaggctcgca acaaccagac caagcggcgc cagcagaatc ggcaggagcg caccgtggag
     6961 gccaatggac agtcgccaac gagagcgcag cgcgatgaaa aggatgttcc acagccccca
     7021 caaattcggc acgatggcca tggggaacac gatcacgcgc tccacgggga caggaaagtc
     7081 gtagacatac cgcaagacac aataaccgga catgatgacc aacagaaata ccgttggcac
     7141 ggcgatccct gccagatacg ctcgaagata agtgtgctga ttcattagaa cctcctgcgt
     7201 tacaagtgat aaagcaatgc cgggatagct cccgggaaga ggaaaagaac cgcgctgaga
     7261 atcgcggcaa gcgcccaatc gaaccacgga actttgtcca cacgggccgg ctgttcatcg
     7321 agcttgcgca gcatttgcgg ccacagatcg cgacggagtt cggcgtcctt taccggggcg
     7381 acggctgcct tgaggagcgc ttcgatttct ttgttgtttt ctttgttggt catttcgcgc
     7441 cgacctctgc acccaacgaa tggagtttct tgcgcaagat gcgcacggct cgaaacacgc
     7501 gcatcttcac cagagccgtg gagattccca tcgcttccgc gatcttgtta tacggttcct
     7561 cttcaatcag cgccaaggtg gccactaaac ggtattttgc cggcaactgg aagaaagcct
     7621 gtttgatccg atcacgcgtc tcgcggcgga ctgcgggatc cgcgacacca gcgctggcca
     7681 ggtcgtccgg gagttcggtt tcgcggcgcg cggtcctcag atgatcgaaa gcggcgttgg
     7741 tcgcgatcct ccgcgcccaa gcgcgaaaat tgccggctgg gcggaaccgc aaccgggacc
     7801 ggtaaatgcg ccagaaagtt tccaccgtaa gatcctctgc aattccatgg tcgcggacga
     7861 tgcgaacaat ccaggcgtag acttccttct ggcgctctcg aaacagcgct tcgaaggctt
     7921 ccaggtcgcc cgccgcgaac cgctccaaca attccatctg ccactctcta tatctactta
     7981 ctggtttttg gcgcccaggg ttacagcttt ttttaggtcc tgcctcttcc agtcatggga
     8041 ggaaattcag tctgagggca agcagaaaga aattaaaaag aaaaaggccc ggacggtcaa
     8101 tgcgcccggg cctattggtt tctgccgaac caaaatcagt agatgtcgta ctgttcccag
     8161 tgcaacgtgg cgtcggactg gatgatgatg tgcttgtgcg cggtttgctc gagttcgtca
     8221 atgagcatgg actcgcgggt cttcagagct ttggcgattt caggattaac gcggatcgta
     8281 aggttcggac cttcgtgatc ggcggcagcc atcttccgcg cttcggactg gatttcgtag
     8341 cagagcgtgg ggatggactt gaccatgccc gagccggtgc agtacgggca gggttggcag
     8401 aggacgcgct cgagggcctg cttggtgcgc ttgcgagtaa tgcagacgag gccgaattcg
     8461 ttgaaggaaa gcgcctttga gggggccttg tcttcctcga gggcctgttg cagcgcggac
     8521 aaaaccttct cgcggttgcg gcgctcctcc atgtcaatga agtcgacaac gatgatgccg
     8581 ccgaggtcgc gcaggcgaat ctggcggacg atttccttga cggcttcgag gttggttttg
     8641 acgatggtgt cttcgaggcg cgtggagccc ttgccgacga atttgccggt attgacgtcg
     8701 atggccacga gggcttcggt gtggttgatg acgatgtagc cgccggactt gagccagact
     8761 ttggcgcgca gcgctttgtc gagctcgtgc tggatgccga actcttcgaa gatcggcgtt
     8821 tccttggtgt aaagcttgac catgttgacg agcttcggct ggaaacggct gacgaactcg
     8881 acgatcttgg tgtactcctc ctcattgtcg atccaaatgc cggtgaaatc gtcgctaacg
     8941 taatcgcgga ggatgcgctc gacgaggttg aggtcgcggt ggagcagaga gggcgctttg
     9001 cgctgctcgc tgcgttcctt gatttcgttc caggacttgc cgagaaactc gatgtcggtg
     9061 cgaatctcgt catcggtggc gccgcctgcg gcggtgcgca cgatgaagcc tccgggatag
     9121 gcaccgccgg attcgctaac taaccggcgc aagcgcgagc gattttcagc ggaaatgatt
     9181 ttgcgagaaa cgccggtgtg atgaaccgtg ggcatgtaga ccagaaaacg ccccggcaga
     9241 gcgacgtggc tggtgatccg cgcgcctttc ttgccgagcg gctccttggc gatctggatg
     9301 acgacatcct ggccggcctt cagcatatcg gagatgagct gtggacggcg cgatgggccg
     9361 gaacggcctc cctgatagcc ccggtcctgc ctggggccac tgggacgtcg ttcgaaacgc
     9421 ggtccgccgc tgatggggcg tccgccgcta tcgggacgcc caccacgact atcgggacgg
     9481 ccgccacggc catcgggacg gccaccgcgg ctatccgggc gtccgccacg gctatcgcgg
     9541 ccacggtcgc gcccaccgct gcggaacggc cgctggaagc gcgaacgcgg gttgccgccg
     9601 atgcgcgcgg agtcgcgcgg aaaatcttca cgtggagccg ctgggccgcc aacgcgcggg
     9661 gagcgagttt cgccggggag gagaatcacg tcgtcctcga tggccggcgt gccttccgcg
     9721 acggattcgc cgggggccgc agagatcact tcctcttcga cgtgctccgt cccggcgtgt
     9781 tcgtcctcga tgtgttcatg ctcgccgtgt tcgtgcgtac tatgcgtggc gtcgtcttcg
     9841 gcgtgctctt gagccacatc cgcggcgaat tccgcccggt tggccgcacg tccggcttcg
     9901 acttcctcga cttcttcctc ggagagtccg gaaacttcag cggccggggt ttcttctgct
     9961 tcttccgttc cttcttccgt ttgctcttcc gtctcttctt cgtcgaattc ggcgccgcca
    10021 gcgatggcgt cggcttcggc ttcttcggtg gcctcttcgt gtttggcctc ggcgaaatcg
    10081 gcggccagtg ccgcaacctg atcttcactt agatcggcat cgctacgaac aggcgcggat
    10141 tcttgctcgg ccggagcttg cggcgtttcg gcagcttgac cggctgttgc ggaaggcgct
    10201 tcgggttcag cgccggcatc ggccaggagc cagcggggca acccgccgga gaatcggcga
    10261 ggaacgctgc tgccgctcgg agcggttgcg ggcagattgc cggtcgtgcg cgatgtaatt
    10321 tcttcgactg tactctgctc ttcgtgtgtt tcgtgctcaa cagtgggggc aggcgcgggc
    10381 gcaactgcag gcttgccgcg atattttgcc agcgattcac ccggaagaat gatttcttcc
    10441 tcggtgacac ttgaagatgg aggcgccggg ctgcgcggag cttcgggacg ccgattgtcg
    10501 taactgcgcg gctctccgcc gcgattatca tatccgcggg actcgccacc ttgtggtgat
    10561 gcatatttcg agggaggcag attgcgcccg cctggggcac ccggcggacg tccgccgcgc
    10621 gggcgtccgc cacggcgtcc ccaacggcca ccccggccgc gatctccgcc acggtcaccg
    10681 ccgcgatcgc tgccgcggtc accgccccgg ccaaaattct ggcctggacg gtcgctaccg
    10741 ctgcgggcgg gatagttttg cgtaggattg tagcgcggcg cgaagttctg gggcgcggaa
    10801 gcgttggaat ccggctggcg ttcctctccc gcctcggcgt gctgatcttc catggggggg
    10861 gacggaacta cgtcgtgatg ttcctcgaga tgttcgccat tcgtggcgtg cgcttcctcg
    10921 tgggcagcgt gctgttcatc ctcaggatgg gtttcgtcct gatggaactc ttggtggtga
    10981 gcttcatcgt gatgatcgcc agcccgggcc agggattcac ccgggagcat ttccaccggg
    11041 gaactgctcg cgggcggcag cgacgccacg gaggcttgcg gctgctcgtg cggatgcccg
    11101 tggtcgtagt cctcgaggtt ttcgaaaaca tcgctgacgt acaggaaggc gtcgccgtcg
    11161 agaccaatgt ccacgaaggc agactgcatg ccggggagga cgcgggtgac ttttcctttg
    11221 tagatgctgc caacgagcgc gaattctttt tcccgctcga tgtagatttc gactaactgg
    11281 ccttcttcga gaatcgcgac ccgccgttcg tgtgaggccg cggagataac caattctttc
    11341 gacatgaaaa ctccttatgc gtgatcccgg catggccgga atcacccttc tgtgcggccc
    11401 cggctaggcc gggaccgaca ggacgcatcg ccgcgagctt ctgcccgtcg gagttccgaa
    11461 aaatcggtgc cgctcccggc aatgccgggg tgcacgttgg ccacacttgt agggaagtgg
    11521 cttggcgttc gaagccagag gggtgggccg cgaccaggtc ggggccacct acgagatgat
    11581 tcctcagtct gtcgaggctg gtccgtcgcc ggaaacgagc cgggcgatcg gcggacgatg
    11641 ctgtcctcac tctaaaatcc ttcttacctg cgaccccagc cgagccggga tcagcaattc
    11701 ccaatgcaaa ttctattccg gtgcgctccg aggaagcgga aacgtcacac ggcactggcg
    11761 gcttggtcag ttgacgaagc ggcgaagccg gacattcatc accaggccaa tggccaggaa
    11821 aacaaacaac gtggcggacc ccccgtaact catcaacggc aacgggatgc cggtaaccgg
    11881 catagcgccg atgaccattg ccacgtttac cagcacatgg aacccaagcg ccgctgcgac
    11941 gcccataacc agaaacattc ccgcgcgatc tttcgcacgt tgagcattct gcactaaacg
    12001 cagcagaagc gccatataca gcccgagggc caaaagcacg cccttgaatc cctgttcttc
    12061 agcccacgcc gacatgatga aatccgaata gcggacggga atgtagccca gttggttctg
    12121 gctgccatta cggaagcctt tgccccaaaa gccgccggaa cccacggcaa tcttcgattg
    12181 caaaagttga tagccagagc ctttggcgtc ttcttcaggg tgcaggaacg aagtgatgcg
    12241 atcccgctga tatggcttaa ggatacgctt gctaacggga ggatagaaaa cagcgcccac
    12301 cagcaaaatc cccgccaacg aaaacaccgc cgcgtgttgc cactgcaagc ccgccagaaa
    12361 tgcgccgaca accaacaggg ggatcagcac gagtgccgtt cccaaatcgg gctgcttgag
    12421 tatcaagacc agcggaccac ctaccaacaa tcctgctttg atcagatcac gcagatcgag
    12481 ctgatcggag cgcacttccg cgaagaagcg cgccagcaca atgattataa tcaatttgac
    12541 caattctgac acctgaacaa gttcgcccaa agccgggatg gcgatccaac gcttggcgcc
    12601 gaaatgggtg tgcccgacca gcagaaccgc gacaagagct gcgaggccaa tgaggtagag
    12661 aatcggagct tggtcgagaa tcagatgata atccaggcga gaaagcacga acatcaagag
    12721 gaagccgatg accagccagc gaatctgctt tgtgtgcatg cccgtaagcg agctgccgtg
    12781 cgtggcggaa tagatttcca tcacgccgag cgtgcagatg gctgcgagga tggccaggag
    12841 ccaccagtcg taatcgcggg ttccgggtgc gtctttcatg aaggaagtcg aaagtccaca
    12901 attgaaagtt gagagaaaag aaaaagtccg ctggtgctga aggcacggac ctgattcctt
    12961 cgctcttttt tagacgaaat cataaaatct ttcagcgttg tggctccgcg ctggcgcgtg
    13021 cggggacaac cgcgttgtcc tccggcttca aggctgcccg cggagcaatc gctgctgcag
    13081 ttgccgagcc actcccggaa tcagaccgct tgttttcagc ggtgacttga ccctgggtct
    13141 tcttggtttt cttgtcgtaa taggccttga tgacttcttt gactacgggc ccggccgctt
    13201 cgccaccatg ttttccgctt tcctgcacca gcacggagac gacaatttcc gggttgcggc
    13261 gcggcgcgta gccaacaaac caggcgttgt cttcgaattt cttttgcttg cccattctgt
    13321 cgcgcgttgc gtaaccaatc acttgggccg tccctgattt tccgcttact tcaattcccg
    13381 gcagtctgac gcctttcgca ttgcctcctt catcgttcac tacgccatac atgccgtccg
    13441 ttattttttc cacggttggc tcggagagcg caaatcgctc ttcgccaacg ttcggcgcgt
    13501 ccttcaggag gtggggttgt ttgaaaacgc cacccatcgc gattccgccg atcgtccgag
    13561 cgagctgcag gggagtcgtc gtcacggcgc cctgcccgat agacaccgag atcgtctcgc
    13621 ctgcatacca cttgcgatga aagacgcgct cggcccattc agcggatggc atcagcccgg
    13681 gttcttccga gggcagatcg attccggtct tatgtcctaa gcccagttta ctgccgtagt
    13741 aggaaaggcg gtcaatgccc aagcgcattc caacgttgta gaaaaaaatg tcgcacgact
    13801 gaaggatcgc tttgtggaag cccaccacac cgtgagagct cttcccgtaa acccagcact
    13861 tgaactgccg gccgtagaaa gtggcgtagc ccgggcaaaa cgtcgtgaaa ctttccggag
    13921 ggtttttgtc ctccaacatg gcggtggcgg tcacgatttt gaaaacggat cctggcgcga
    13981 gctgcgcctg aatcgcgcgg ttgaacaaag gatgtagcgg gtcttcgttg agactcttcc
    14041 agtcatctcc cgaaatgcgc accgcgaact cgttggggtc caaggcggga tggctcacca
    14101 tcgccaggac ctcaccggtg tgcgggtcca gcgcgactac cgcaccaggg cgcggcccca
    14161 aggactgctc cgcgatctgc tgcaagtcgt agtcgatggt tagctgaatc tgtttgccgg
    14221 gaatagcttc ttgggtggat aggcgctcca cttccttgcc gatgctgttt acgaccacgc
    14281 gccgcatacc gtccgtgccc tgaagcgagt cgttgtactg gcgctccaga ccggtctttc
    14341 cggcgaagtc gccggggcgc agcttgccgt tgctcgcttc gatctgctgc tcgctgactt
    14401 caccaacata tcccgcagca tgagcgagga agccgccagg caggtaacgc ctgcgagaga
    14461 cggaaatcat ttccaggacc ggcaggtccg aacgatgtga ttcgatgaag gcgatgtccg
    14521 ccggcgaggc gtcgggtttg atgatgatgg gctggaattt cgggagattc tttgtattcg
    14581 tgagctggtc cctcaaatca tccagaggga ttcccaggcc gtccgagata cccgggagat
    14641 gcttttccac cagagctgga tcatcgcgaa ggaggagcac gctgaacgcc ggtcgattgt
    14701 ccaccagcac gcggccatcg cggtccagca tgcggccgcg cggcgcaatc acgggaatgt
    14761 agcgcacgcg attgcgctcg gccatcgagc tgtacttgtc ggcgtcgatg acctggagtt
    14821 tccagaagcc gagcagcagc acgccgatca ttcccacgat gatgtaggaa gccacggcta
    14881 gccgcccttg cggcaatcga ttgtcgttgc ggaaccagta cgacacgaaa accccgcgcg
    14941 caagtatttc cagggctttt ggcccggtcc ggcccaattt gactaaactg gccggactaa
    15001 actcgcgtca cgaaaaaatc cacagcgtgg gtttcgagtg gaccccacat aaccagggcc
    15061 tagccgaaaa tgtccgtcga aaatttcctc gaacctgggc tacgaaaaaa tctcccggag
    15121 ctcccaaatg ccaccggact aaggccgcgt gaagcagcaa aagtctttct aaacgtacca
    15181 cctgcctgag cgagccgcaa gccgtcccag aggacaggtt ccagattgga actgacctac
    15241 gcgcggtcgc ggcgttgcgc ttgttgccgt ggctgtgagt atcacgctat cgtaggcaat
    15301 tcacctatga ccgctgaaac gaacagggaa aagccgctgc ggctggcact aatcggcatg
    15361 tctggtgccg ggaagacctt ctgggcaaag aagttagccg caagccggta tcccgcggtt
    15421 tcttgcgacg accgcatcga gcaaaagctc gccccgcgcc ttgccgccgg cggattcgcg
    15481 ggcatcaatg gtgtcgccgc gtggatgggc tggccgaact ccgcgacgta caccgaacgc
    15541 gagtccgagt acttggctga ggagattcac acgctggatg aaatcttgac ggaactcgag
    15601 aagcagccgg agaaatccct ggtgctcgat accaccggaa gcgtcattca caccgggaac
    15661 aatttgctga tgcgcttgcg gcgccaaatg acaatcgtgc atctggcggc ttccgccgaa
    15721 gagcgacagc tcctgatcga aagatatttg aacgatccaa agccggtgtt gtggcgcggc
    15781 gcgttccaac cgagggcggg agaaaaacca cgcgagacag tggcgcgatg ttatccggcg
    15841 ctgattgccg cgcggcgtca aagttatgaa gcgctcgcac attgcacgct gcaagttgcc
    15901 gcgttgcgcg atggatcgct ggacgctccg gcgtttctga aaatgattca ggacaagacg
    15961 ggaagcccgc gatgagtgca attcattgag ataccggagc acatcgcgga aggccccgct
    16021 cacctcgctt cgcggcgcgg tgctgcgcgg gcttgcgccc gacggcggcc tgtacatgcc
    16081 ggtggagatt gcgcggcatt cacccgagga actggaggaa tttcgcgggc tgccgtttac
    16141 ggaagtgtgt ttccgcgtgg tgcggccgtt tgccacgccg gatgcacctg aagaagtgct
    16201 ttggcaaatc gtgagcgagg cgatcaactt tcccgtgaat ctggtatcgc tctcgcccgg
    16261 cctgcacatt ctggagctgt ttcacgggcc gacgctggcg ttcaaggatt tcggcgcgcg
    16321 cttcatggcg cggctgatgg gttatttcgt tcgcggcgaa acgcgcactc ttacggtgct
    16381 ggtggcgaca tccggggaca ccggcagcgc cgtggcgcac ggtttcctgc aggtgccagg
    16441 aatccgcgtg gtgattctct atccatcgaa gcgcatcagc gaagcgcagg agaagcagtt
    16501 caccacgctc ggcgaaaaca tcaccgcgct ggaagtggcg ggcacgttcg acgactgcca
    16561 gcgattggtg aagcaggcct tctccgatgc ggagctgaac aagcacacgt ggctgacttc
    16621 cgcaaattcc atcaatatcg ggcggctgct gccgcaaatg ttttatcacg tggcggctta
    16681 ccggcagttg ccggtggcgt ccgttccgtt gatcgtttcc gtgcccagcg gaaacttcgg
    16741 caacctcacg gcgggaattt tcgccaagcg catcggcctg ccagtagcga aatatgtggc
    16801 ctccacaaac gccaacgatg tcgtgcccga atatctacgg acgggagaat ttcatccgcg
    16861 ctcggcgcag gcgacctatt cgaacgccat ggatgttggc aatccaaaca attttccgcg
    16921 attgctcgat ttgtgccgca accgtctcga atacgtgcag aaggaaatct ggggccacgg
    16981 cgccacggac gaagaaacgc tcgcggcgat gaagatgctc cacgataagt tcggctacat
    17041 cgccgatccg cacacggccg tcggcgtgct cggttgggaa gcatacaaga gccaacatcc
    17101 cgaacccgca cagggcttgg tcctggccac agcacatccc gcgaaattcg ccgacgttgt
    17161 aatgaaagcc ataggcactg cgcccccgct gccggatcgg ctcgccgcct acttgaagcg
    17221 cgagaagcta tctctgccca tctcgagttc ctacgacgaa ttcaaacagt ttcttctcac
    17281 ccgataaaca gcgaagaatt cacgctgcaa tccagtggcg accagagtgt gacgaatcgc
    17341 tagttcttgc ggaggcggtc gagcagaagg aagaggaaca cccccacgag cgcgttgacc
    17401 agcgccgcca cgaagaggtg catgttaaac cacggctcca gttgcgcgag caagatgcgc
    17461 cgggtgagaa ccatcaggcc ttgatgcgcc acgtagaaag cggcggtgag cgcgaaccgc
    17521 gcggcgggat gctcggtatc gagccttgcc ccgatcgagg aagcgagata cccgatgatg
    17581 gttttcgcga tgccgtagag tcccagtgga accgtggggc cgctcaggct gtcttgcagc
    17641 agaccaatga acatgcccag gagaagtccg gtggaaggat tgcgccggct gaggccgaaa
    17701 taaatcgtca ccagcaaagg caaatcgagg acggcgacct ttgcaaaatg aatcgggaac
    17761 gaggcctgga ttaccagcgc cagcagcgtg gcgatgatga tcgcgcccgc gcgaaacttg
    17821 tgaacttcga tgtgcgattc gccgcggata tcaaacgcgt cgttcatcgt tttgccgacg
    17881 gaggcggatt tgctgcgccg ggcgcggatg gcagcgcatc cctggtattt tcttcatttt
    17941 tcatttcgag aggatgcagc gacagaagaa ccagcacttc ttccagacgc tcgagattgg
    18001 cagccggttt gatgcgaatc tgcttgaacg gattgccggc cttgatttcc gcgacggtac
    18061 ccacagggag atcgcgcggg aagatgcggt ccatgccgct ggtaatgaca cgttccccga
    18121 gattgaccgt gtcgtcgttc gggacgtatt tcatgaccag cagaggctca ccctgccccc
    18181 caacggggct ctggatgcgg gaatcggcca gcatcgcgcc gacgccgctc tccttgtccg
    18241 tcagtaaaag tacctgcgat gcattcggat aggtttcgac gactttgccg accacgccat
    18301 ccggagtgat cacgcccata ttttttcgaa tgccgtcgcg ctcgccgcgg tccagttgaa
    18361 tcgtcaggct cgcggttccc gcaccggtgg caatcacccg cgcggcaatc atggggacgt
    18421 ccgcatgcga ttgccggaag ttcagcaata ccgctatgcg gtcggcttcc gcggctttgc
    18481 cctgaagctg tgaaatttgc aatttcagcg catcgttctc atggcgaagc tgctcgtttt
    18541 ccttcgtcgt gttttgcagc gcaaagtagt ggcgccaggt gtcgtgaatc cagccgaagc
    18601 cgtgggagcc agcccgctca aagggcgaaa cggcaccgac cgtccagacg cggatgaggc
    18661 gtccctgcga gggtcgcttg atctgcacgg ccagcataag cacctgcagc acgatgactc
    18721 ctgcgagcag gaccagcgat ttgtggcgag agggaatggc taccatgtag attccgaaat
    18781 tggaaactcg aaattggaaa atcgaaacgc gaaacttggt ttcattgcga atcaaacccc
    18841 agggggcggc atgttgtgtc cctacagcgg cagattctca atcgatgcaa acctgacgca
    18901 gcaatttgaa atcgctcagc atttttcccg tgcctagcac caccgacgcc agcggatcat
    18961 ccgcaatgga aaccggcaag ccggtctctt cgcgaatccg cttgtcgaga ttcttcagca
    19021 gcgcgccgcc tcccgtcagg acgataccgc ggtcgctgat gtcggcggaa agctcgggag
    19081 gtgtgcgttc gagagccacg cgcacggcgt tgagaatggt cgcgacgcat tcgctcaggg
    19141 cttcgcggat ttcgctgtcg tcgatggtga ccgtccgcgg cacgccctcg atgaggttgc
    19201 ggcctttcac ttccatcgtg agcggttttt ccagcgggta ggcggagcca atctccatct
    19261 tgatctgctc ggccgtgcgc tctccgacga gcaggttgta cttgcgcttc agatactgca
    19321 tgaccgcttc gtccatttcg ttgcccgcta cccgcaccga ccgcgaataa acgatgccgc
    19381 tcatggagat gaccgcgatg tccgtggtgc cgccgccgat gtccacgacc atgttgccgg
    19441 agggctcttc aatcggcagc ccggcgccaa tcgccgcagc catggcctgt tcgacaagaa
    19501 aaacttcgct agcgcgcgcg cgatacgcgg agtcctgcac ggcgcgcttt tccaccggcg
    19561 tgatctcgct cggaacgccg ataacgattc gcggatgtac cagcacgcgc cggttgtgcg
    19621 ccttctggat gaaataggtc agcatcttct cggtgacttt gaaatcggcg atgaccccgt
    19681 ctttcatcgg cttgatggcc acaacgttgc ccggcgtgcg ccccagcatc tcttttgctt
    19741 cgcggcccac ggccacgact tctccggtgg atttgttgat tgccacgatc gagggctcgt
    19801 tgacgacaat gcctttgccg tgcgcgaaca ccagcgtgtt tgcagtgccc agatcgatgg
    19861 cgaggtcgga ggaaaacaga ctgaataccg acctcaggtt gtaaccgttc ttagtcatcc
    19921 ctacatcccc ctgcggagaa agactctccg ctgcccggcc ggcaatagcg ctgacctcac
    19981 cgcggccagc tccaacccct attgtctccg gacaccacga ccagcgcaaa ggcgcttggg
    20041 tctttacttc ggaactctta cttcggaacc acgatggaca cttgcttgat cgccgttccg
    20101 ccccggcgat tcgacccgat aatcgaaatc gtgtgttgcc ccggatcgag cgattcggtg
    20161 aaatgccgga atgctccatc cggtgagacg ttcggcaccg gttgaccgtt cacgatcagc
    20221 gccgctcccg gttctgtccg gcccacgatc tccgccacgt gtccatgcaa ctgggttccg
    20281 tcaatctcga gcagcatggc ctgcgacttg ccttgcgcga cgagcgtaaa tttaaaaatt
    20341 tcgctgactt cgctggtttg tttctttcca tccgtcgccg tgacattcca gaagtagtcc
    20401 ccgggatcga gcccggtgat ctccaccgcc gttccgccga ccttcgcgtc cttcaccgtc
    20461 ttcgtaaaca tcgcggtggt gctgatccgc agcgtatacg acacggcatc ctgaacgggc
    20521 ttccattcga atcgcaccgg cgagatcttc ggattttccg cgatgatcgg cgcgaggttc
    20581 agcggctcga cgagctcggg aggcgccagc acatttgatt tctgaatcgg tccgccgctg
    20641 ggaatcgtga tcttctccca cggcgcgagg t
//
LOCUS       DASYVO010000030         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_269166, whole genome shotgun sequence.
ACCESSION   DASYVO010000030 DASYVO010000000
VERSION     DASYVO010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269166"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VGR03_01120"
     CDS             complement(<1..475)
                     /locus_tag="VGR03_01120"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV1992912.1"
                     /translation="MSEDIIGTRKGDYEILGVLGVGGMGKVYKVRNVLSDRIEAMKVL
                     LPNLADQKDLADCFLREIKVLASLSHPNIAALQTALTIDNQLVMIMEYVEGQTLSARV
                     GQGAIPLDEALNYIGQVLAALSYAHKQQVIHRDIKPANMMLTPEGVIKLMDFGIAR"
     gene            complement(792..866)
                     /locus_tag="VGR03_01125"
     tRNA            complement(792..866)
                     /locus_tag="VGR03_01125"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Phe,seq:gaa)
     gene            complement(938..1408)
                     /locus_tag="VGR03_01130"
     CDS             complement(938..1408)
                     /locus_tag="VGR03_01130"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEV1992913.1"
                     /translation="MNSRIKFGLLAMLLLAGMGVSFAQKASGSADEQTIRKLDKEWSA
                     AVQSKDAGKMLAYYAADASAFPFNAPIATGKEQIQELWAGLMSMPGFSLSFAPTKIEV
                     AKSGDMAYDVGTFELKHNDANGNLTTTAGKYVVVWKKQPDKQWKVVADIFNTDK"
     gene            complement(1488..>2066)
                     /locus_tag="VGR03_01135"
     CDS             complement(1488..>2066)
                     /locus_tag="VGR03_01135"
                     /inference="COORDINATES: protein motif:HMM:NF020952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV1992914.1"
                     /translation="ILATQRPSVDVITGLIKANFPARISFRVASKVDSRTILDSNGSE
                     SLLGKGDMLYLPAGSARLHRIHGPLVSEDEITSVCDFWREQAQAKYQEELLAAPKDEN
                     GKTEGAADGDAAGGEDVDDDLYQDAIRVVCDAGRASTSTLQRRLRIGYGRAARLIDLM
                     EKDGIVGPPDGTKPREVLKNRSWMKEFDDSSR"
BASE COUNT          407 a          626 c          549 g          484 t
ORIGIN      
        1 aacgagcaat gccgaaatcc atcagcttta tcacgccctc cggcgtcagc atcatgttcg
       61 caggcttgat gtcccggtgg atgacttgtt gtttgtgcgc gtagctcagt gcggcgagca
      121 cttggccgat gtagttgagc gcctcatcaa gagggatcgc tccttgtcct acgcgggcgc
      181 tcagcgtctg gccttcgacg tactccatga tcatgaccag ctggttgtct atggtgagcg
      241 ccgtctgcaa cgcggcgatg ttcggatgac tcaggctcgc cagcaccttg atttcgcgca
      301 agaagcagtc ggccagatct ttctggtccg ccaggttcgg cagaaggacc ttcatggctt
      361 cgatgcggtc cgacaaaacg ttccgcactt tgtaaacctt gcccatgccg cctacgccga
      421 gcactcccag aatctcgtag tcgccttttc gcgtgccgat gatgtcttcg ctcatttcac
      481 cactcccgga gaaactttcg atggcttagg atactgcatg tcccgtccgg gaaatacaaa
      541 aagtccccgc ttcggcggat ggaggccaaa gcgtatagtg ccttctatcc gcatctccgt
      601 gaatttgagt tttccgcggt gaacaaagcc acacgccagc agaattgcca tccccgccgc
      661 ggcaatttct ttccttcaac tgaaaactga gcactggcaa ctgaccacac cctccctatc
      721 tgcacttgaa gaatgacggt tctgttcggg tggggcttgt agcctggcta aagccagaaa
      781 gcagttgcgg atggtgccgg gggccagaat cgaactggct acgcccgcct tttcagggcg
      841 gcgctctacc aatgagctac cccggcattt tggaacattt caattttaga gacgatagcg
      901 agcggtgtca attcggctcg cggctggcca gcttgcccta tttgtccgta ttaaagatgt
      961 cggccacaac tttccactgt ttatccggct gtttcttcca cacaaccaca tatttgccag
     1021 ccgtggtcgt gagatttccg tttgcatcat tgtgtttcag ctcaaaggtt ccgacgtcgt
     1081 aagccatgtc gcccgacttt gccacttcaa tcttggttgg agcaaagctg agcgagaatc
     1141 cgggcatcga catcaagccc gcccagagct cctgaatctg ctccttgccc gtcgcgatcg
     1201 gagcattgaa cgggaacgcg gacgcgtctg ccgcatagta cgcaagcatc ttcccggcat
     1261 ctttgctctg caccgcggcc gaccattcct tgtccagttt gcgaatggtc tgttcgtcgg
     1321 cgcttcccga agctttctga gcgaaagaaa ctcccattcc agcaagcaat agcatcgcca
     1381 atagtccgaa ctttatcctc gaattcattt gcggctcctc cgctctttga atacacgcat
     1441 ggtcgcctct gtggcgcaga ttttagcaca cggaccggga atgtctctca gcgcgaggag
     1501 tcgtcgaatt ccttcatcca actacggttt ttgagtacct cgcgcggctt ggttccgtcg
     1561 ggcgggccga cgatgccgtc cttttccatc aggtcgatca agcgggcggc gcggccgtaa
     1621 ccgatgcgca agcggcgctg caacgtggaa gtagaagcgc ggccggcgtc gcaaaccacg
     1681 cgaatcgcgt cctggtagag gtcgtcgtca acatcctcgc cgcctgcggc atcgccatcg
     1741 gccgcacctt cggtcttgcc attctcatcc ttcggggccg cgagcagttc ctcctggtac
     1801 ttcgcctggg cttgttcacg ccagaaatcg cataccgatg tgatctcatc ttcactaacc
     1861 agcgggccat gaatgcgatg aagccgcgcc gagcccgccg gcagatacag catgtcgcct
     1921 tttcccagca gcgattccga cccgttcgaa tcgagaatgg tgcgcgagtc cactttgctg
     1981 gccacccgga acgaaatgcg ggcagggaag ttcgctttga tcaaaccggt aatcacgtcc
     2041 acggaaggcc gctgcgttgc gaggat
//
LOCUS       DASYVO010000031         3790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_85187, whole genome shotgun sequence.
ACCESSION   DASYVO010000031 DASYVO010000000
VERSION     DASYVO010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85187"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VGR03_01140"
     CDS             complement(<1..758)
                     /locus_tag="VGR03_01140"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEV1992915.1"
                     /translation="MSADTNRDAIAKLIQSLDTVATRNHLTQDPPAQTGELLHFKLRH
                     AGVPALEIEIVTRATPASSLGAKNSSGKAADARLAIIIDDLGSDRAAAEAIFALGYPL
                     TISVLPNHEHSLEIAQEAHRRGFQVMLHLPMQSIANDTPEARELHPGMPAAEVAALVD
                     QFLQNVPDAAGVNNHQGSEATADAALMDELMPVLRDRHLFYVDSRTIAASVAYDTAQD
                     FGVRSAFRNVPFLDDVAEVAAVRKQLELALRGAR"
     gene            complement(1062..2300)
                     /locus_tag="VGR03_01145"
     CDS             complement(1062..2300)
                     /locus_tag="VGR03_01145"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEV1992916.1"
                     /translation="MNRAARIGILCVSVAIFCYAGIGHVLGRTQDDKAYKSLTVYGEV
                     LQKVQQDYVDDPNMRTVTAGSLHGMLESLDSQSSYLTPREYDEYKKKLTNAGTAETGL
                     TLSKRYGYIIVLSVVPDSPGTKAGIRSGDIFESISGFTTRDMSVGQAVNLLNGQPGTG
                     IKVAVIRRGKATPEEVDLVREKLATPKIIAQKVDPDILVLRIPSLDAGRADEIRNRLL
                     DAEKQGIRKVILDVRECGRGQVSEAISTARLFVPSGTLTTLRGQTVSTQVFSAEPKQV
                     IWKNPVSVLIDATTSGAAEVLASAIAANHRGDVVGERTFGLASEQKLITLDDGAALIL
                     TVANYYNPGGKSIMEEGVTPTEVVRAVPEDDSDTTDEEAAPAPQTQKEPGLGPRPLSP
                     EDPIFHRALDLLKAPAKKAA"
     gene            2562..3470
                     /gene="lpxC"
                     /locus_tag="VGR03_01150"
     CDS             2562..3470
                     /gene="lpxC"
                     /locus_tag="VGR03_01150"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEV1992917.1"
                     /translation="MFQTTVARESFAEGVGLHTGVYGHLRLVPAPAETGIVFRRVDLD
                     NFPIEAQGHNVARVSYATSLMKQGVLLSTTEHLLAAIYSCGIDNIYIDIDSIEVPILD
                     GSAEPFMQMLEHSGVRKLRRKRRYLRILKPLEVIEGDRRIGIYPADEFRVRCYVDFPH
                     PLVGQQEVEMLVGPDTFRHLLARARTFCFQRDIEPLKAMGLIRGGSLENAIVLTNDGV
                     MNGPLRFPDEFGRHKALDLIGDLALAGLPLLARVEAHKAGHALHTQLVSRLLADPSLW
                     TITTGEATRPESNAYLTALSPAPATD"
     gene            3541..>3790
                     /locus_tag="VGR03_01155"
     CDS             3541..>3790
                     /locus_tag="VGR03_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV1992918.1"
                     /translation="MSNNDLAIVILAAGKGTRLKSSLAKVLHRAGGRTLVEQIVRACE
                     LLKARETVVVVGHQAEQVAAVVEPLGALTVLQQPQNGTG"
BASE COUNT          766 a         1121 c         1108 g          795 t
ORIGIN      
        1 tcgcgggcgc cgcgcagcgc caattcgagc tgtttgcgga ccgcggccac ttcggcgacg
       61 tcgtcgagga acgggacgtt tcggaaggca gagcgaactc caaagtcctg cgccgtatcg
      121 taagccaccg aagcggcgat cgtacggctg tcgacgtaga agagatgacg gtcgcgcagc
      181 acgggcatca gctcatccat cagcgccgca tcggctgtcg cctctgatcc ctggtgattg
      241 ttgacgccgg cggcgtccgg aacattttgc agaaattgat cgaccagcgc cgcaacttcg
      301 gccgcgggca tgccagggtg cagctcccgc gcttcaggcg tatcgttcgc gatcgattgc
      361 atcggcagat gaagcattac ctggaaccca cggcgatgcg cctcctgcgc gatctccaac
      421 gagtgctcgt gattgggcaa aacggaaatg gtgaggggat aacccagcgc gaagatggct
      481 tcggctgcag cgcggtcgct gccgagatcg tcgataatga tcgcgagccg cgcatccgca
      541 gcttttccgc tggaattctt cgctcctaga ctggacgcgg gtgttgcgcg cgtcacgatt
      601 tcgatttcga gtgctggaac gcctgcatgt cgaagcttaa aatgaaggag ttccccagtt
      661 tgagcgggag gatcctgcgt cagatgattt cgggtcgcca cggtatcgag actctgaatg
      721 agctttgcga ttgcgtcacg gttcgtgtct gcggacaaat cattcttcaa cacgatacgc
      781 aggtaatccg tgtttccggg ttgctggctg gaagcgcctt gccggagttt aatgggggtc
      841 ccggttggta ccgcagtaga agccgctgcc gctagatcgc gcgtgatttg atgaacttgc
      901 gcgggcgtga gacggttctc ttcgtttttt ttgcaaccac tggaaagaac cgcgagaatg
      961 aagcaaatcc ccagcagtgc gatacttgtt aaaaagttcg tcgtgattcg aaagcaatgc
     1021 aggcttgatg gaaccgagtc aggctgctgg gccacggaaa actaagcagc ctttttcgcg
     1081 ggggccttca agaggtcgag cgctcggtga aagatggggt cttccggaga aagcgggcgc
     1141 ggcccaagcc cgggttcctt ctgggtttgc ggtgccggcg ctgcctcttc atcggtcgta
     1201 tccgaatcat cttccgggac ggcgcgcacc acttccgttg gcgtgacgcc ttcttccatg
     1261 atcgatttgc cgccgggatt gtagtagttc gcaaccgtga ggatcagtgc cgcgccatca
     1321 tccagcgtaa tcagtttctg ctcggacgcc agaccgaacg tgcgctctcc aaccacatcc
     1381 ccgcgatgat tcgcagcaat ggcggaagcc agaacttccg cggcccccga cgtcgtggca
     1441 tcgatcaaaa ctgagacggg attcttccaa atgacctgct tgggctcggc cgaaaaaacc
     1501 tgtgttgaga ccgtctggcc gcgcagcgtt gtcagagtgc ccgaaggaac gaaaagacga
     1561 gcggtcgaaa tcgcctcgga gacctgtccg cggccgcatt cccgcacatc cagtatgacc
     1621 ttgcgaatgc cctgtttttc cgcgtcgagg agacgattgc gaatttcatc cgcgcggccg
     1681 gcatcgagcg aggggatgcg caggaccaga atgtcgggat ccactttctg ggcgatgatc
     1741 ttcggcgtag ccaatttctc gcgaacgaga tcgacttctt ccggcgtggc cttgccgcgc
     1801 cggatcaccg caaccttaat gcccgtgccc ggctggccat tgagaagatt tacagcctgg
     1861 cccacggaca tgtcgcgtgt ggtgaatccg ctgatggatt cgaaaatgtc gccgctgcgg
     1921 attcccgcct tcgtgcccgg actatccggc accacggaga gaacgataat gtaaccgtag
     1981 cgcttgctga gcgtcaggcc ggtttccgcc gtccccgcat tggtgagctt tttcttatat
     2041 tcgtcgtact cgcggggcgt gaggtagctg gattgagagt ccagggattc cagcatcccg
     2101 tgcaaggaac cggcggtcac cgtccgcatg ttggggtcgt cgacgtaatc ctgctggact
     2161 ttctgcagaa cctcaccgta caccgtgagc gacttatagg ctttgtcgtc ctgcgtgcgg
     2221 cccaggacgt gtcctatacc tgcgtagcaa aagatggcta ccgatacgca aagaatcccg
     2281 atgcgagccg ctcgattcat gccccaccct tccacaagta actaccaatt tcaattgtac
     2341 caccgtaaag gattgggacg cggcaccaaa cggaaaggta gcctgacaag ggagcaatgt
     2401 tgccggacac cgccagtgat gagacacgtc gaagccgaca aatgttgcgc aattaccaca
     2461 ctgacctggt tctgggatgc aatgtcactt ataaaccatt gataataaag cacaagctag
     2521 tgtggggttg gcgaacaaca tgctaggatg gacctcggga aatgttccag actaccgtcg
     2581 ccagagagtc tttcgccgag ggtgtgggcc tccatactgg ggtctacggt cacctccgtc
     2641 tggtacccgc tccggccgaa acaggcatcg tgtttcgccg tgttgacctg gacaatttcc
     2701 ccatcgaagc gcaggggcac aacgttgctc gcgtcagtta tgccaccagc ctcatgaagc
     2761 aaggagtctt actctccacc accgagcatc tgcttgccgc catctattct tgcggcatcg
     2821 acaacattta catcgacatt gactccattg aagtgcccat tctcgacggc tcggctgagc
     2881 cgttcatgca gatgctggag cactccggtg tgcgcaaact gcgccgcaag cggcgctatt
     2941 tgagaatact gaagccgcta gaggtcatcg aaggcgatcg ccggatcggc atctatcctg
     3001 ccgatgagtt tcgcgtgcgc tgttacgtgg attttccgca cccgttggtg ggtcagcagg
     3061 aagtagagat gttggtgggg ccggatactt tccgccacct tctggcgcgc gcgcgaactt
     3121 tctgtttcca acgcgacatc gagccgctca aggccatggg cctcattcgc ggcggatcgc
     3181 tcgagaacgc catcgttctc accaacgatg gtgtcatgaa cgggccgttg cgcttccccg
     3241 acgagtttgg acggcacaaa gcactggatc taattggcga tctcgcgctg gccggattgc
     3301 cgcttctcgc tcgcgtcgaa gcccacaagg ccggccacgc cctgcatacc caacttgtca
     3361 gccgcctctt ggccgatccc tcgctttgga ccatcaccac gggcgaagct acccgccccg
     3421 agagtaacgc ctacctgacc gccctctctc ctgccccggc cactgattag ggattcgccg
     3481 cgtcgtaagc aacccttcat tgcgaaatag tccccgctcc cgcagaatgg gctcaaaacc
     3541 atgtctaaca acgatctagc cattgtgatt cttgcggccg gcaaaggcac gcggctgaag
     3601 tccagcctcg cgaaggtgct gcaccgcgcc ggtggacgca cgctcgtcga acaaattgtg
     3661 cgcgcctgcg aactgctgaa ggcccgcgaa accgtggtcg tggtaggcca ccaggcggaa
     3721 caggtcgcgg cggtggttga gccactcggg gcacttaccg tactccagca gccgcaaaac
     3781 ggcactggcc
//
LOCUS       DASYVO010000032         5594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_198702, whole genome shotgun sequence.
ACCESSION   DASYVO010000032 DASYVO010000000
VERSION     DASYVO010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198702"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..455)
                     /locus_tag="VGR03_01160"
     CDS             complement(111..455)
                     /locus_tag="VGR03_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992919.1"
                     /translation="MKKEHILQEIKRTAEANGGVPLGWRKFAKETGNREADWLGKHWA
                     RWSDALREAGCTPNEMTTAYDESFLFQKFIELARELKKLPVKGDFLMKARHDSAFPHC
                     KTFEKFGSRQSL"
     gene            complement(550..2181)
                     /locus_tag="VGR03_01165"
     CDS             complement(550..2181)
                     /locus_tag="VGR03_01165"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEV1992920.1"
                     /translation="MRGTMMDFPLTLPTILERAGKIFPRVEIVSRRPDKSVARSCYGE
                     FYRRARRLAAALTKLGLQPGDRVASMMWNHSGHLEAFFGVPCAGGILHTLNLRLHPHE
                     IAGIARHAGDRFLLIDDVLLPVYEKFREAFSFERVIVVPFGCNTVPEGFSNYEDLLAE
                     AGDDFRYPRIDENDGAAMCFTSGTTGHSKGVIYSHRALVLHSFCCGLNEVFGISQMDT
                     VLPVAPMFHANAWGLPFACTMLGARLVLPGPNVDPQSILDLLVSERVTVACGVPTVWI
                     AVLEALEKNHGRSKFDNPIRVLIGGTAPPLELIRKLDRFGLQIKHLWGMTETTPVGTS
                     GGLRPHMRDWPEDKKYEVRSKQGWPAPFVEIRLMGPEGPEGKSGEVPWDGVTSGEIEI
                     RGPWVAASYHNSPDQAHRWTADGWFRTGDVATMDEDGYVKIVDRAKDLVKSGGEWISS
                     VDLENTLMGHPAVKEACVVGIPHPKWQERPLAAVVLKDGARATAEELRAFLAQSFAKW
                     QLPDAFVFIDAIPRTSVGKFKKIALREQFANWKWE"
     gene            2458..2847
                     /locus_tag="VGR03_01170"
     CDS             2458..2847
                     /locus_tag="VGR03_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV1992921.1"
                     /translation="MKKLYIGNLPFSATEDQLNEWFSQIGVTPSAVNLIRDRFTGQSR
                     GFAFVEVTNDEDADRAVNSLNGQNFGGRNLVVNEARPQAERGGGGGGGRGGYGGGRGG
                     HGGGGGGGRGGGGGGRGGDRGDRGNRW"
     gene            3411..3686
                     /locus_tag="VGR03_01175"
     CDS             3411..3686
                     /locus_tag="VGR03_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992922.1"
                     /translation="MTTWHKRDWEQFYELARRPWRRHRPPRPIYPTGLNRVLPAQGFS
                     LSELDDAGVDLDLAERLGLPVDAGRVGAYGPNVTVLRDFIRSSRQPM"
     gene            4187..5068
                     /gene="lipA"
                     /locus_tag="VGR03_01180"
     CDS             4187..5068
                     /gene="lipA"
                     /locus_tag="VGR03_01180"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEV1992923.1"
                     /translation="MPTPAPGTHPRPDWLRVKFFGGERYQDLKHIMRTLDLHTVCESA
                     RCPNMGECWEHGTATFMILGDICTRACGFCAVPSGKPVGPPDEEEPFRVAEAVAKMGL
                     RYAVVTSVNRDDQPDGGAHIFARTIKEIRRRVPGCKVEVLIPDFRGDWNALDIVLAVR
                     PDILNHNTETVPRLYRQVRKGAVYERSLELLRRSKEAHPEVPTKTGLMLGLGEEKEEV
                     LATMQDLAAQGTNILTLGQYLQPTREHLPIVRYVHPDEFTEYKVLGEAMGFKHVEAGP
                     LVRSSYHAFDQAESAVK"
     gene            complement(5148..>5594)
                     /locus_tag="VGR03_01185"
     CDS             complement(5148..>5594)
                     /locus_tag="VGR03_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1992924.1"
                     /translation="AKGWKPLFLTVSFVGTDELILEAGADAEGMVITQVVPPYYLTDY
                     KTVALYRRTLTKYFPSTQPNFVSLEGFVDAIVLVEGLKKAGKDLTREGLIRGIESIHD
                     HDLGLGPQLKLNYSSKEHKGLDHVIPTVIRGGRAVPFTDWTTATPK"
BASE COUNT         1132 a         1602 c         1703 g         1157 t
ORIGIN      
        1 ggaagggtca gccgcttctg aatcgttatc ggcacgcgaa acgtgacctt cacacaagag
       61 cacaatgtct tcgtatcctt cgcgattctg gcagtactcc ttcaccttct tcacaagctc
      121 tgccttgagc caaacttctc aaaggtcttg cagtgtggga aggcagaatc atgtctggcc
      181 ttcatgagaa aatcaccctt gacgggaagt tttttcagtt cgcgtgctag ctcaatgaat
      241 ttctgaaaaa gaaaagattc atcgtaagcc gtagtcatct cgtttggagt gcagccagct
      301 tcacgcagag catcgctcca tcttgcccag tgcttaccga gccaatccgc ttccctattg
      361 cccgtctctt ttgcgaactt gcgccaaccc aggggcacac cgccgttggc ttctgcggtt
      421 cgctttatct cttgcaggat gtgttctttc ttcatcagtt aaacggatta tacatgccct
      481 ctccaggatg ttgaagacgc ggtcgggttc gttggaatgt gggcagagag acgcaggctg
      541 aagcctgcgc tactcccatt tccagttggc gaattgttcg cggagggcaa tttttttgaa
      601 tttgccgacg gaggtgcggg gaatggcgtc gataaagacg aaggcgtcgg ggagctgcca
      661 cttggcgaag gattgcgcga ggaacgcgcg caactcttcc gcggtggcgc gcgcgccgtc
      721 tttcaggaca acggcggcga gcgggcgttc ttgccacttc ggatgcggaa tgccgacgac
      781 gcaggcttcc ttgacggcgg gatgacccat taaggtgttt tcaagatcga cggagctgat
      841 ccattcgccg ccggatttca cgagatcttt cgcgcggtcc acgatcttga cgtagccgtc
      901 ctcgtccatg gtggcgacgt caccggtgcg gaaccagccg tcggcggtcc agcgatgcgc
      961 ctggtcggga gaattatggt agctggcggc gacccacggg ccgcggattt cgatctcgcc
     1021 ggaggtgacg ccatcccacg gaacctctcc tgatttgcct tcggggccct ccggccccat
     1081 caagcgaatc tcaacgaagg gcgccggcca gccctgcttg gatcgaactt catacttctt
     1141 gtcttcgggc cagtcacgca tgtgcggccg gagaccgccg gaagtgccga cgggagtggt
     1201 ttcggtcatg ccccagagat gcttgatctg caggccgaaa cgatcaagct tgcggatgag
     1261 ctcgagaggc ggcgcggtgc ccccaattag gacgcggatg gggttgtcga atttcgagcg
     1321 gccatggttt ttctccaagg cctcgaggac ggcgatccaa acggtgggga caccgcacgc
     1381 gacggttacg cgctcgctga cgagcaaatc gagaatgctt tgcggatcga catttggacc
     1441 ggggagaacc aggcgggcgc cgagcatggt gcatgcgaaa ggcaggcccc aggcgttggc
     1501 atggaacatg ggcgcgacgg gcaagacagt atccatctgg ctgatgccaa agacttcgtt
     1561 gaggccgcaa cagaaggaat gcaacaccag cgcgcgatgg gaatagatga cgcctttgga
     1621 atgaccggtc gtgccggacg taaaacacat cgccgcgccg tcgttttcat cgatccgcgg
     1681 atatcgaaaa tcatcgccgg cttccgcgag cagatcttca taatttgaga aaccttccgg
     1741 cacggtgttg cagccgaatg ggacgacaat cacgcgttcg aatgaaaaag cctcgcggaa
     1801 tttctcgtag actggcagga gcacgtcatc aatcaacagg aaccggtcac cggcatgcct
     1861 ggcgatgccg gcaatttcat gcggatggag ccgaaggttc aaggtatgca aaatgccgcc
     1921 ggcacacgga acgccgaaga atgcttccaa gtggccggag tgattccaca tcatggaggc
     1981 gacgcggtca ccgggctgca atccgagttt cgtcaatgcc gccgcgagcc ggcgcgcgcg
     2041 ccgatagaat tcgccatagc agcttcgcgc gacggacttg tctggtctgc gggagacgat
     2101 ctcgacgcgc ggaaaaatct tccccgcgcg ctccagaatg gtgggcagcg tcagcggaaa
     2161 atccatcatc gtgccgcgca taaggcctct cgggcgggga gtaaagtggc cgaatcctat
     2221 cactaattca cggtaaagag gaaaggtgcg gcagcgcggt tcggggcaat gcggcccgct
     2281 ggcgcgggac aatttcagtt gcaaaagggg ttaatttccg agaggatgca acagaccgct
     2341 gaagccacgg tctcgcgcct gcggtggccc ggttttccgg actgccggta aaggatagct
     2401 gtctcttcag cgcgctccgc gactcgcatt ttaggttccg ggcagaagga gtaagaggtg
     2461 aagaagctct atatcggtaa tcttccgttt tccgcgacgg aggatcagct caacgagtgg
     2521 ttttcgcaga tcggtgtaac tccctcggcc gtcaatctga ttcgcgatcg gtttacggga
     2581 cagtcgcgcg gcttcgcctt cgttgaagtg accaatgacg aagacgcgga tcgcgccgtg
     2641 aattccctga acggtcagaa tttcggcggg cgaaacctcg tggtgaatga agcacgtccc
     2701 caggcagagc gcggcggcgg cggtggtggc gggcgcggcg ggtacggtgg cggacgcggc
     2761 ggccatggcg gtggtggcgg cggtgggcgc ggcggcggtg gtggcgggcg cggcggtgat
     2821 cgcggcgacc gtggcaatcg ctggtaggca agtacttcga gtctgaaagc gtagcgcccc
     2881 ggccccggtc ggggcgcctc agtcctctct gggaatcctc agtggtatct ctgcgtcaat
     2941 catccgtcgt tgcagctctc acgttcgccg gttcagttcg tccaatacga gagtagggta
     3001 gtcggcgagg aattgctgcc cgcggacaag actccgcgaa cgggacatcg cgctggttaa
     3061 catacgcgaa tgtgcgtagt atcccgcggc atgtaaccgt cttggcggtt ggtcagcagg
     3121 aacacgacgg gagtctggcc gcaatgaaga cgctccccgc tccttcttcc ctggcctgtt
     3181 tcgcgaaaca atttgccccg taacgtgttt gaaacaacag gcaaggaaaa taggctgccg
     3241 gcccgtttat gaagtatatg aataggccgg agaacatccg tgctgttcca tggagaaggg
     3301 atgcgcggca ggaggctggt gaaccttatc ggccgggcgt gcatccaaag ggttgaggcg
     3361 aacgtattac caggtaggtt gagcccgagt gagtcgggat cgagcaagaa atgacgactt
     3421 ggcacaaacg cgattgggaa cagttttacg agctggcgcg ccgcccttgg cggcgtcacc
     3481 gcccgccgcg gccgatctat ccgacgggct tgaatcgggt gctgcccgcg cagggtttca
     3541 gcttgtccga gctggacgat gcgggtgtgg atctcgacct tgccgagcgt ctcgggctgc
     3601 ccgtggatgc cgggcgcgtc ggcgcttacg gccccaacgt caccgttctg cgcgatttca
     3661 ttcgttcctc ccgccagccg atgtaatcct gctttcaaag gcaatgctct ttctgatttc
     3721 ggctgtcctt tctgattgag cctctggaat ctgctccatc tcgagtcccc ccttttcatt
     3781 tccacgccgt ccaaaagaag gcttgaccct ggaatggatt ccagggtcca tacttctgat
     3841 gtttgggaga ctgcttgtgg accagaaaat gcttcgctcc ggtaagctgg cccagttgac
     3901 gggagtcagc acggacctgc tgcgccacta cgaacgcatc ggaattctgc ctcctccggc
     3961 gtgcgcatcc gcatcacgac caagaccccc gaggcgcttc aggccgtgca taagtttctc
     4021 cgttttcaga tcgccgacca tccaacgggc gacgccacgg aaataacaaa agtaccatag
     4081 gtcctttcgc ttggcagttt gtacgatgtg ctggatgagc gtaaaataag agtttcacgc
     4141 gagtcccatg gccacaccct tcaacatcat ctccaacgca acggcgatgc cgacgcccgc
     4201 gccgggcaca catccgcggc cggattggct gcgcgtcaaa tttttcggtg gcgagcgcta
     4261 tcaggatctg aagcacatca tgcgcacgct cgacctgcat acagtctgcg agagcgcgcg
     4321 ctgccccaac atgggcgagt gctgggaaca tggcaccgcg acctttatga ttttggggga
     4381 catctgcacg cgcgcttgcg ggttctgcgc cgtgccttcg ggcaagccgg ttgggccgcc
     4441 ggatgaagaa gaaccatttc gcgtggcgga agccgtggca aagatgggat tgcgctacgc
     4501 ggtggtgact tccgtgaaca gggacgacca gcccgacggc ggcgcgcaca tttttgcgcg
     4561 caccatcaaa gagattcgcc gccgcgtccc cggctgcaaa gtcgaagtgc tgattcccga
     4621 ttttcgcggt gactggaacg cgttagacat cgtgcttgcc gtgcgcccgg atattctcaa
     4681 ccacaacacg gaaaccgtcc cgcggcttta ccggcaggtc cgcaaaggcg cggtgtatga
     4741 gcgctcgctc gaactgctgc gccgttccaa ggaagcgcat cctgaagttc cgaccaagac
     4801 tggcctgatg ctcggcctcg gagaagaaaa ggaagaagtc ctcgctacca tgcaggacct
     4861 cgccgcgcag ggtactaaca tcctcacgct gggacaatat ctgcagccca ctcgcgagca
     4921 tcttcccatc gtccgatacg tgcatcccga tgaattcacc gaatataaag tgctcggcga
     4981 agcgatgggc ttcaagcacg tcgaggccgg cccgctggtg cgctcttcct accacgcctt
     5041 cgaccaggcc gaatccgcag ttaaataaca gcagcaattt tgcgcgtgcc tcttcgggct
     5101 ctcgtctgcg tgagatgcaa aggagagcag tgattgtatc gcttgaacta tttgggcgtg
     5161 gccgtggtcc aatccgtgaa gggcacggcg cggccgccac gaatcaccgt tgggatcacg
     5221 tggtcgagac ctttgtgttc tttcgatgaa taattcaatt tgagctgcgg cccgaggccc
     5281 aggtcatgat cgtggatgga ttcgataccc cgaatcagcc cttcgcgcgt cagatccttc
     5341 cctgccttct ttagtccttc caccagcacg atcgcatcga caaagccttc caggctcacg
     5401 aagttcggct gcgtggaggg gaagtacttg gtcagcgtgc ggcgatagag ggcgaccgtc
     5461 ttatagtccg tcaggtaata cggcggcacg acctgagtga tcaccatgcc ttccgcatcg
     5521 gcgcccgctt ccaggatcaa ttcgtccgtg ccgacgaacg ataccgtcag aaagagcggc
     5581 ttccaaccct tcgc
//
LOCUS       DASYVO010000033        15801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_15227, whole genome shotgun sequence.
ACCESSION   DASYVO010000033 DASYVO010000000
VERSION     DASYVO010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 15801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15801
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15227"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2708
                     /locus_tag="VGR03_01190"
     CDS             <1..2708
                     /locus_tag="VGR03_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV1992925.1"
                     /translation="RRDRKTGDEDDIIRQLRSAFRPVLSPDGKQLLYVTRYETETGLR
                     LRNLETGEDRWVKYPVTRDDQESRFTRDVFPGYTFLPGGKEAVYNQDGKIKRLDLATG
                     TDKVIPFTAQISQELGPKLDFPQKVEQGPVKVRLIMDPVESPDGKKLAFSAMTHLYTL
                     DLPSGTPQRLTSGNAHEFQPAWSPDGKTIAYVTWSSEGGQLWKKSATGGSPVQLSKSL
                     AVYSNPAWSPDGTRIVLLRGNAYDRENSTFDGGQTANADLVWVPADGGDASLILPARG
                     AGGPHFTQEKDRIYVYTPQGLVSLRYDGTDRRTHIQVKGQGAFAEEPVPADDMQPSPD
                     GQWVLAQAMNQLYLIAMPVVGGEAPTVNVTSPTVPVKRLTDIGADYFAWADGGKTITW
                     AVGASLFREPLSAISFEPPKDEKKDGDKKEADAKGSDKKDATSQTVDAKKDEKKEEKK
                     EEKKLKEQEKDVQEIAVNLEFPRKTPKGTIVLRGATVVTMNGDEVLKNADIVVENNRI
                     KSVGAKGGVPAGAKIFDVSGKTIVPGFVDTHAHWTEIRRGILDTQNWSFLANLAYGVT
                     AGLDVQTGSNDMFAYQDMVDSGDILGLRAFSTGPGVFYDNNFQSAEEVKGVLTKYKKY
                     YGTHNIKSYVVGDRKQRQFMVQAAKELEMMPTTEGALDLKLNLTHVIDGFHGNEHTLP
                     ITPLYNDVIQMFAKSGIAETPTLIVNYGGPFGEDYWFENSEVHDNTKLNHFMPHRVID
                     ERTKRRPGWFRKDEYEFPKLAAQMTKLERAGGLVGVGSHGEMQGIGYHWELWMLASGG
                     MTPLEIIRCATVNGSKIIGRPADLGSIEPGKLADLLIMDKSPLDDIHNTNTIHWVMKN
                     GELFEGDTLNQIWPEQKKLEPLWFWNNYDAPKPGEPLNYGKTTQP"
     gene            complement(2826..3785)
                     /locus_tag="VGR03_01195"
     CDS             complement(2826..3785)
                     /locus_tag="VGR03_01195"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1992926.1"
                     /translation="MRYLVTGGAGFIGSNTVDELVRRGHSVVVLDDLSSGKEDNLAEI
                     RNKITFIKGSITDIEVVRKAMHEAEYVLHLAARTSVPRSVKDPIETNKINIDGTLNVL
                     VAAKELKVKRVVFAASSSAYGETPTLPKVETMQPQPISPYGVTKYVGELYGQTFGRCY
                     GLENVALRYFNIFGPRQDPGSPYSGVLAKFCTAFLEDTQPLVFGDGEQTRDFTYVENT
                     VQANLLACEAPNVSGKVFNIGVGGRVSLNGVLRELGKITGKALEAKYDPPRDGDIRDS
                     QADISQAREYLGYEPQVTFEEGLARTFEWYRETQAKAAAKAEK"
     gene            4027..4917
                     /locus_tag="VGR03_01200"
     CDS             4027..4917
                     /locus_tag="VGR03_01200"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEV1992927.1"
                     /translation="MSRKTTVGLAAFAALGCAAGAFYWLHVRGQQPPPEVVSPEPIGF
                     NDPPFLEKPAKAPAAKSPLPAESKKSTPPADTGLPLRAGEVLEYTANVSKLENVATLR
                     LQVAGRGNFLGKSTWHLQAFAHTQNPLRMVFELDDQFDSYSDAGTLASLQYEMHLSER
                     GQKVESVQRMTATGKEPAPPDATQTRVLPGTRDPLGMMQYLRNVDWTKTPEVRSPVYD
                     GHKLYDLRAKLAPGSATVNVPAGNYTASRIAIRIFEDGVEKKDASFTLYLANNAARTP
                     VLLEAVMPFATARVELQKAK"
     gene            complement(5175..6776)
                     /locus_tag="VGR03_01205"
     CDS             complement(5175..6776)
                     /locus_tag="VGR03_01205"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEV1992928.1"
                     /translation="MNRRTFLQAGASAAIGALAGSGQAFAADAEIELSPGEPGAVISP
                     QIYGHFIEHLGGVIYDGVWVGRNSSIPNVDGIRKQFVDDMKRIGAPNLRWPGGCFADG
                     YHWRDGIGKAAGRPRTYNFWQSRMPAGIDATETNQFGIHEFMHLCRLVGAEPYVAANV
                     GSGTPGEFHDWVSYCNAPVGTLSLADERAANGDKQPFGVRYWGVGNESWSCGGTMRPE
                     EYAAHYRRFVTQFPAYTEPFLVATGPRGHSADGDIRWTTGFFEAMRGALPPHGFSLHF
                     YTDLRPTPLKAGDFKAPEWYEVLLRGVRLDKVIEDHWKEMGKFDAAHRTKLVVDEWGV
                     WYAPGSEITPAYILSETIMLRDAVHTGVTFDIFNRHADKIAMANVAQTVNCIHSLFLA
                     QGDRFVRTPVYHVFDMYRAHMGARQVPVSNPFPELNVPALAGQARLPGLSASASIRNR
                     RLAVTLTNPSLDAALSVRIRLAGGARATQARGLVLTHQDMRATNTFADPDEVKPAGHP
                     VRVAADALEVSLPKQAIILVECDIT"
     gene            complement(7169..7594)
                     /locus_tag="VGR03_01210"
                     /pseudo
     CDS             complement(7169..7594)
                     /locus_tag="VGR03_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
     gene            complement(7755..9110)
                     /locus_tag="VGR03_01215"
     CDS             complement(7755..9110)
                     /locus_tag="VGR03_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEV1992929.1"
                     /translation="MPIDRKPEFFKGAQLKVGIIGCGYVGLPLALRFAEAGHKVTGFD
                     VDPHKVTMLNAGKSYIEHIPQNKIQQFVNSRHFSATNDFAKLKDADAILICVPTPLDE
                     RREPDLSYVEQTAISIYPHLQRGQLVVLESTTYPGTTEELVLPILEKSGLRCAIAAGP
                     ENENIACDFYLAFSPEREDPGNKQYGLAQIPKVVGGLNPPSGRAAQALYTQVVSRVVP
                     VSSTRAAEMAKLLENIFRCVNIALVNELKQLSLRMNIDIWEVIDAAATKPFGFMPFYP
                     GPGLGGHCIPVDPYYLSWKAREYDFSTRFIELAGEVNTAMPYHIVDAVANSLNDHKKS
                     MKGSKILVLGVAYKKDVDDLRESPTLKIMQLLQQRGAILDYNDPYFPKLHKMRHYNYE
                     NMKSVPLNPQTLGSYDAVIIATDHSSYDYVAIVDAAKLVVDTRNATRRVMRHREKIVL
                     C"
     gene            9399..9803
                     /locus_tag="VGR03_01220"
     CDS             9399..9803
                     /locus_tag="VGR03_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV1992930.1"
                     /translation="MTFIILLLGLGVGVLVGLLGIGGGVVLVPAMVYLLHMDQHLAQG
                     TSLFILLPPIGLGALREYWKQGQVDLNAGILCALGMLFGAYAGSLIALPMPSRHLKGA
                     FGCFLMLAAFLLWRKTHRDTNVIGSDEEKARV"
     gene            9796..10179
                     /locus_tag="VGR03_01225"
     CDS             9796..10179
                     /locus_tag="VGR03_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV1992931.1"
                     /translation="MSDAMGRGAGIFAIACVCGVAAGMFGVGGGVLLVPLLGLLFAFS
                     QHRAQGTSLIALIPPTGVLALMAYSKAGFVSWKTGLLLIPGVFLGGIAGGKVAKWIKP
                     RMMRQIFAGILFALGALQAFSAWWH"
     gene            10195..10965
                     /locus_tag="VGR03_01230"
     CDS             10195..10965
                     /locus_tag="VGR03_01230"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEV1992932.1"
                     /translation="MVDLHCHILPGLDDGPANMEESIAMAESAIADGITHVVATPHSS
                     NEYHFGFTHTRQLRDELQAKVGDRLKIATGCDFHLNPENLASLLRDARQFCINQRDYL
                     LLEFNEFSIPPSMDQTLHEIQLAGVQPVITHPERNGILRSHPERLNKWVRQGCFAQVT
                     GGALTGGFGAGAQQDALRWIGEGLIHFVASDAHNTRTRPLRLQPAYNVVVDRFGQEKA
                     RALFLDNPLAAFEGRELPHVPEIEDELSPSRRKRFFFF"
     gene            complement(11102..11767)
                     /locus_tag="VGR03_01235"
     CDS             complement(11102..11767)
                     /locus_tag="VGR03_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEV1992933.1"
                     /translation="MANPDNPATVFRPRSRTRRVLFAVGGGGLVLAVVIFFGVGRWLV
                     VEDPLVKARAIVVLSGAMPLRAIEAAKLYREGYAPEIWLTHSTEPGETLEEMGIPFSG
                     EDYYNKLVLIHEGVPPEAIHVLETPIVNTADEIKVAATALAQRKDSAVILVTTKAHTH
                     RVRLLWRRLALGQGRAIVRAASGDPFDPRHWWRTTSDALDVVRELLGLLNAWAGLPLH
                     PAQ"
     gene            complement(11833..12378)
                     /locus_tag="VGR03_01240"
     CDS             complement(11833..12378)
                     /locus_tag="VGR03_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992934.1"
                     /translation="MSPKSSTSVSATIDPHCQHISPKGNRCHMLIDEHHRHSNGAKRP
                     ALCAYHANRLKAAVPVVDPEVLAADLLGDIDGFTTADEVNLFLGNLVKQLARKRIARR
                     DAIALAYISQLLLTSQTAMAREAVAEKEAEDLYRNLVQGLSRSRSSSSGEQQSPDNAA
                     TTCPGSELAAGPGTEHARRAS"
     gene            complement(12871..13326)
                     /locus_tag="VGR03_01245"
     CDS             complement(12871..13326)
                     /locus_tag="VGR03_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEV1992935.1"
                     /translation="MPRQLTSNEHLDYVAALLDDMFRIPGTNIRFGLDALIGWVPGIG
                     DAMAGIASFLIVFAAWRRGVQSITLVRMIANVVLETTLGAIPVAGDIFHVFWKANRRN
                     YRLLIREKEQPGSNTRRDWMFLTIILFTAIAAAGIPIGILIWILRSHLI"
     gene            13377..13820
                     /gene="fdhE"
                     /locus_tag="VGR03_01250"
     CDS             13377..13820
                     /gene="fdhE"
                     /locus_tag="VGR03_01250"
                     /inference="COORDINATES: protein motif:HMM:NF016131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HEV1992936.1"
                     /translation="MLLFHRTRLRGESRWRAAQAAKTPVVATTSVCPLCGARSLLGVL
                     RPEGDGGKRFLLCSFCSQEWEFRRILCPTCGEEAENKLPVYIAEQLPHIRVEACDTCQ
                     FYIRTVDLTKDGHAIPLVDDLSAIPLTLWAHEKGYSRLQPNLLGT"
     gene            complement(13871..14350)
                     /locus_tag="VGR03_01255"
     CDS             complement(13871..14350)
                     /locus_tag="VGR03_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992937.1"
                     /translation="MFDTGPLWELVLYSAVHTLRFESLKSELRHLRSDSSYQSLSEFV
                     ASFQRKTTTPHVVAEISSWIRRRTERKGQSAIWGLVYDEFSSMGMDEGVLKLLEMPQE
                     LVADMGAVDVSVLKLGLSLGQAKPLVLSIDSELIAECKRAGVNAKDLWEVIAEGIPQ"
     gene            complement(14390..15088)
                     /locus_tag="VGR03_01260"
     CDS             complement(14390..15088)
                     /locus_tag="VGR03_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992938.1"
                     /translation="MSGRLEMPPKKQSRRVAAWIYVVINPIIDSLQRELSLLDSGNLT
                     WRPHLGRCEMIRTIQEYVDSTQWPNYKDFLAEHPKSAFIPGFQRHDSDLKKLNTVAQE
                     LFNRMLSWEQFLVSVGAALDIYESGRASLGPRAASLTDTRNDIPKMAAEYLINNAQTL
                     PSHYVISAFWNSAGNSLIGFRNLPEFQPLHRSKDGLSEISAKLKQALEGYRLSLSRDY
                     DVPAAPVPGISLEQ"
BASE COUNT         3377 a         4505 c         4667 g         3252 t
ORIGIN      
        1 cgcggcgcga ccgcaagacg ggcgacgaag acgacattat tcgccaactc cgaagcgctt
       61 ttcgcccggt gctctctccc gatggcaaac agcttctcta cgttacacgc tatgaaacgg
      121 agaccgggct tcgcttgcgc aacctggaga ctggtgaaga tcgctgggta aagtaccccg
      181 tgacgcgcga cgatcaggaa tcacgcttta cgcgcgacgt cttcccgggg tacacgttcc
      241 tgccgggcgg caaagaggct gtctacaacc aggatggcaa gatcaagcgg ctcgatctgg
      301 cgacgggcac agacaaggtg attccgttca ccgcgcagat ctcgcaggaa ctcggaccga
      361 aactcgattt tccgcagaaa gtggaacaag ggccggtgaa agtacgcttg attatggacc
      421 cggtggaatc ccccgatgga aagaagctgg cgttttcagc gatgacccac ctctacacgt
      481 tggatctccc atccggaacg ccgcagcggc tgaccagcgg aaacgctcat gaattccagc
      541 cggcgtggtc gccggacgga aagaccatcg cctatgtgac ctggtccagc gagggcgggc
      601 agctctggaa aaagtcggca acaggcggat cgccggtgca gttgtcgaag tcgctggctg
      661 tttacagcaa ccccgcgtgg tcgcccgacg gcacgaggat cgtccttctg cgcggcaatg
      721 cttacgaccg cgagaacagc accttcgacg gcgggcagac ggcaaatgcc gacctcgtgt
      781 gggtccccgc ggacggtggc gatgccagcc tgattctgcc ggcgcgcggc gcgggcggac
      841 cgcatttcac gcaggaaaag gatcgcattt acgtgtacac gccgcagggc ctcgtttccc
      901 tgcgatacga cggcacagac cggcggacgc acatccaagt caagggacaa ggagcttttg
      961 ccgaggaacc cgtgccggca gatgacatgc agccgagtcc ggacgggcag tgggtgctgg
     1021 cgcaagcgat gaaccagctc tacctgatcg ccatgcctgt cgtgggcggc gaagcgccga
     1081 ccgtgaacgt gacgtcccct accgtcccgg tgaagcggct gacggacatt ggcgcagact
     1141 attttgcctg ggccgatggc ggaaagacca tcacctgggc ggtgggcgcg agccttttcc
     1201 gcgagccgct gagcgcgatc tctttcgaac cgccgaagga tgaaaagaaa gacggcgaca
     1261 agaaagaggc ggacgccaag ggctcggaca agaaggatgc cacttcgcaa acggttgatg
     1321 cgaagaaaga cgaaaagaaa gaagagaaaa aagaagaaaa gaaactgaag gagcaggaga
     1381 aggacgtcca ggagatcgcg gtcaatctgg aattcccgcg gaagacgccg aagggcacca
     1441 tcgtgctgcg cggcgcgacg gtcgtgacga tgaatggcga tgaagttctg aaaaacgccg
     1501 atatcgtcgt cgaaaacaat cgcatcaaga gcgtgggcgc gaagggcggc gtgccggcgg
     1561 gtgccaaaat attcgacgtg agcggcaaaa ccattgtgcc cggtttcgtc gacacgcacg
     1621 cgcactggac cgaaatccgg cgcggcatcc tggacacgca gaactggtcg ttcctcgcga
     1681 acctggctta cggcgtgacg gccgggctcg acgtgcagac cggttcgaac gacatgtttg
     1741 cataccaaga catggtggac agcggcgaca tcctcgggtt gcgcgcgttc tcaaccgggc
     1801 ccggcgtttt ctacgacaac aacttccagt ccgccgaaga agtcaaaggt gtgctgacca
     1861 agtacaagaa atattacggc acgcacaata tcaagagcta cgtcgtgggc gatcggaagc
     1921 agcggcagtt catggtgcaa gcggcaaaag agctggagat gatgccgacg acggaaggcg
     1981 cgctggacct gaagctcaac ttgactcacg tcatcgacgg cttccacggc aacgagcata
     2041 ccctgccgat tacgccgctt tacaacgacg taatccagat gttcgcgaag tcggggatcg
     2101 cggaaacacc gacgctcatc gtgaattacg gagggccgtt cggcgaggac tactggtttg
     2161 agaacagcga agtgcacgac aatacaaaac taaatcactt catgccgcac cgggtcatcg
     2221 acgagagaac caaacgccgt ccgggatggt tccgcaagga cgagtatgaa tttccgaaac
     2281 ttgcggcgca gatgacgaag ctcgagcgcg ccggtggact agtgggcgtt ggcagccacg
     2341 gcgagatgca gggaattggc tatcactggg agctgtggat gctggccagc ggcggcatga
     2401 cgccgctcga aataatccgt tgcgcgaccg tcaacggttc gaagattatc gggaggcctg
     2461 cggacctggg ctcgattgag cccggcaagc tggcagacct gctcattatg gataagagcc
     2521 cgctcgacga tatccacaac accaacacga ttcactgggt catgaagaac ggcgaattgt
     2581 tcgaaggcga cacactgaac cagatctggc cggagcaaaa aaagctggag ccgctgtggt
     2641 tctggaacaa ctacgacgca cctaagcccg gtgagcctct caactacggc aagaccactc
     2701 agccttagcc atccagcctt caagaagcgc agttgtaggg gcgagcttca gttcgccctg
     2761 tttctctccg ggagttattg cctggccgcc gcgcctgccc cacgcgcctc ttcaccagtc
     2821 accaatcact tctccgcctt cgccgcggcc ttcgcttgcg tctcccgata ccactcgaag
     2881 gtccgcgcga ggccttcctc gaaggtaacc tgcggctcat agccaagata ttcccgcgct
     2941 tgagagatgt ccgcctgaga gtcgcggatg tctccatcgc gcggcggatc gtacttcgcc
     3001 tcaagcgcct tgccggtgat ttttcccagc tcgcgaagaa ctccattaag ggagacgcgt
     3061 ccccccacgc cgatattgaa aacttttccg gaaacattgg gcgcttcgca agcgagtaga
     3121 ttggcctgca cggtgttttc gacgtaggtg aagtcgcgcg tctgttcgcc gtctccgaaa
     3181 acaagcggct gcgtatcttc caggaacgcc gtacagaact tggccagcac gcccgaatag
     3241 ggcgaaccgg gatcctggcg cggcccaaaa atattaaaat aacggagcgc gacgttttcc
     3301 agaccgtagc accgcccgaa cgtctgcccg taaagctcac ccacgtactt ggtgacgcca
     3361 tagggcgaaa tgggctgcgg ctgcatggtt tcgacttttg gaagagtggg cgtctctcca
     3421 taagcagaag acgaggccgc gaagaccacg cgcttgacct tcagttcttt ggcggccacc
     3481 agaacattca aggtgccgtc gatgttgatt ttgttcgttt cgatggggtc cttcaccgaa
     3541 cgtggcacgg atgtgcgggc ggcgaggtgc agcacgtatt ccgcttcatg catggctttc
     3601 cgcaccacct cgatgtccgt gatgctgccc ttgatgaaag ttatcttgtt gcggatttcc
     3661 gccaggttgt cttccttgcc ggaagacagg tcatcgagaa cgaccacgct atggccgcgg
     3721 cggacgagtt cgtccaccgt gtttgagccg atgaagccgg cgccgcccgt gacgaggtag
     3781 cgcattcagg aaatcctcct agctcttgcg gccgccttgc ggccgaggat cgtgtctgcc
     3841 aacgacaggt tgccaccgca gcatatgacg cgagcccggg aaagacgacc ggcccgcttt
     3901 ttgatacgct catagttcgc ggcgagccgg cgaagggagc cggatgattt ccgcgtacgg
     3961 aaagatattg tacgacggaa gcgcgccggg caagcggttg attcaaggga gcgtcacaca
     4021 aagagcatga gccgcaaaac gactgttggt cttgcagcct tcgccgcgct aggttgcgcc
     4081 gcgggagcat tttactggct gcacgtgcgc gggcaacagc ctcctcctga agtcgtatcg
     4141 ccggagccga ttggttttaa tgacccgccc ttccttgaga aacccgcaaa agccccggcc
     4201 gcgaagtcac ccttgccggc tgaatcgaag aaatccacac ctcccgccga caccgggtta
     4261 cctctgcggg ccggcgaagt tctcgaatat accgctaacg tttcgaagct cgaaaatgtg
     4321 gcgactctgc ggttgcaggt tgccggccgg ggcaatttcc tgggaaagag cacctggcat
     4381 ttgcaggcct ttgcgcacac ccagaatcca ttgcgcatgg ttttcgaact agacgatcag
     4441 ttcgattcct acagcgatgc cggcacgctg gcgagtctgc agtatgagat gcacttgagc
     4501 gagcgcggcc agaaagtaga gtccgtccag cgcatgaccg cgacgggtaa agaacctgcg
     4561 ccaccggacg ctacgcagac gcgggtgctg ccgggaacgc gcgatccgct cggcatgatg
     4621 cagtacctgc gaaacgtgga ttggacaaaa acgcccgagg tgcgcagccc ggtctacgac
     4681 ggacacaagc tgtacgacct tcgcgccaaa ctcgcacccg gttccgcgac ggtgaacgta
     4741 ccggcaggga attacaccgc ctcaagaatc gccattcgca tcttcgaaga cggcgtcgag
     4801 aagaaggatg cctctttcac gctgtacctg gcgaataacg ccgcgcgcac gccggtcctg
     4861 ctcgaggccg tcatgccctt cgccactgcc cgagtcgagc ttcagaaagc aaagtaagaa
     4921 gttcgcactc ggccttcgtc ttggctgcaa ctttcaacaa tcccaagaat cggtcatggt
     4981 tcatcagttt ccggacagtt tccgggttgg cgacaaccca cccagaactt ccagtttcgc
     5041 ttcccgcaac cacgtttatc gggcagtgag tatcaacgac acgcacaagg gcatgatagg
     5101 cccgggccca gcgagtatgt ctgcgattcg gcggaccggt cgcgagattc acccggtggc
     5161 ccgcgcgggg gacctcaggt tatatcgcac tccaccagga tgatggcttg cttcggcaag
     5221 cttacctcga gcgcgtccgc ggctactctg acaggatgcc cggccggttt gacttcgtcg
     5281 gggtccgcaa aggtgttcgt ggcgcgcatg tcctggtgag tgagcaccag gcctcgcgcc
     5341 tgggtcgcgc gggctccgcc ggctaaccgg atccgcactg acaaggccgc atccagcgaa
     5401 ggattggtca acgtcaccgc gagacgccga ttgcggatcg acgcggaggc ggacaacccc
     5461 ggcaggcggg cttgaccggc cagtgccgga acgttcagtt ccgggaacgg gttgctgacg
     5521 ggcacctgcc gagctcccat atgggctcga tacatgtcga atacgtggta aacaggagtg
     5581 cgcacgaatc tgtcgccctg cgccaggaac aaggaatgaa tgcaattcac ggtctgcgcc
     5641 acattcgcca tcgcaatctt gtcggcgtgc cggttgaaaa tgtcaaacgt cacgcccgtg
     5701 tgcaccgcgt cgcgcagcat gatggtctcg ctcaggatgt aggcaggggt aatctccgag
     5761 ccgggtgcgt accacactcc ccactcgtcg actaccagct ttgtgcgatg cgccgcgtcg
     5821 aactttccca tctctttcca atggtcttca attactttgt ccaggcgaac gccacggaga
     5881 agaacctcgt accattctgg cgccttgaaa tctccggcct tcagaggtgt gggccgcaag
     5941 tcggtataaa agtgaagtga aaacccgtgg ggaggaaggg ctccgcgcat ggcctcgaaa
     6001 aaacccgtgg tccagcggat atcgccgtcg gcggaatgcc cgcgggggcc ggtcgccacc
     6061 aggaagggct ccgtatacgc gggaaactgg gtgacgaacc gccggtaatg ggcggcgtat
     6121 tcttcgggtc tcatcgtacc gccgcaactc caggattcat tgccgacgcc ccagtaccgc
     6181 acaccgaacg gttgcttgtc gccgttggcg gcacgctcgt cggcgaggct gagggttccg
     6241 acgggcgcgt tgcagtagga cacccaatcg tggaattccc caggcgtacc ggacccgacg
     6301 ttggcggcga cgtatggctc ggcgccgacc agccggcaca gatgcatgaa ttcgtgaatg
     6361 ccaaactggt tggtctcggt ggcgtcgatt ccggcgggca tgcggctttg ccagaagttg
     6421 taagtgcggg gccgccccgc ggccttgcca atcccgtcgc gccagtgata cccgtcggca
     6481 aagcagccgc cgggccagcg cagattcggg gcgccgatcc gcttcatgtc gtccacaaac
     6541 tgttttcgga ttccgtctac gttcggtatg gacgagttcc gacccaccca cacgccgtcg
     6601 tagatgacgc cgccgaggtg ctcgatgaag tgcccataga tctgcgggct gatgacggct
     6661 ccgggctccc cgggcgagag ctcaatctcc gcatccgcgg cgaacgcctg gccggaaccg
     6721 gccagcgcgc cgattgccgc cgaagcccct gcttggagaa aagtgcgtct gttcatgggt
     6781 gtcaacgctg tgtgtcttta ccgactatgc gtctcgatac ggagaatggt aaccgagttg
     6841 gtcgccagcg tgtagtccag ctccccggcc caaaccggaa aagaggcgca taggctgggg
     6901 ctcgaagcgc ttaaagacta ccgcagacac ccttcgcggt tttctacatt tgggatgatt
     6961 cactgatttc acgccggccc aacatcgaaa ttgttcgaac cagccagaag tcccgttctg
     7021 gggaattgcg agttcgcggt ccacttggct gtgtactcgg ataatcggaa actgatgacc
     7081 cactactcaa gaatccgaat caaatctcca tagatcttac ttttggctct caactgtcga
     7141 ttgtaaacct cttcgtttgc gggacagcct acccgcaagc cagttgctca atgaggctgc
     7201 aagcgatgtg gccgatggtg atgtgacatt cctgaatgcg ctgcgggtct tgcgacggaa
     7261 cattcaagag aagatcaacc ttgccggcga gaagcccacc tgtgtctccc gtgaaggcca
     7321 ccagataggc cccgcaagcg cgcccagctt ccactccacg aatgacattg gggctattgc
     7381 cgcttgtcgt gagcgccaca agaatgtcgt gctttgcgac gaggggctca atctggcggg
     7441 agaagatacg ctcgaatccg tagtcgttcc ctgccgcagt cagtaccgaa ggattcgtag
     7501 tgagtgccag agccgccagg cccgggcgtt ccgttcgcag ccgcacaacc agttcggcgg
     7561 caagatgcag cgcatccgcg gcgctgccgc cgtttccaaa aaaaaacgat cttaccgccc
     7621 gcccgcagcg cggctgccga cttctccgcg aattgcgcga tccgcccgat cagttcattg
     7681 gaaaaacttt gtttgacgcg agcgctttcc tcgagctggc ggcgaatctc atcggcagac
     7741 gaagtcattg cagcctagca gagcacgatt ttttcgcggt ggcgcatcac gcggcgcgtg
     7801 gcgttgcgcg tatcgacgac gagtttggcg gcatccacga tcgccacgta gtcgtaactc
     7861 gaatggtcgg tggcgataat caccgcatcg tagcttccca gcgtctgcgg attcagtggc
     7921 accgatttca tattttcgta attgtagtgc cgcatcttgt gcagcttcgg gaaataggga
     7981 tcgttgtaat cgagaattgc gccacgctgc tgcagaagct gcatgatttt cagcgtcggc
     8041 gattcccgca gatcatccac gtccttcttg taagccacgc cgagcaccag aatcttcgag
     8101 cctttcatgc ttttcttgtg atcgttcagc gaattggcaa ccgcgtcgac gatgtgatac
     8161 ggcatcgccg tgttgacttc gccggccagt tcgatgaagc gcgtcgagaa atcatattca
     8221 cgcgctttcc aggaaaggta gtaggggtcc accggaatgc agtggccgcc caggcccggg
     8281 cccggataga acggcatgaa gccaaatggt ttggttgccg cggcgtcgat cacttcccag
     8341 atgtcgatgt tcatgcgcag gctcagctgt ttgagctcgt tgacgagcgc gatgttcacg
     8401 cagcggaaaa tattttcgag cagcttggcc atctccgcgg cgcgcgttga gctcacggga
     8461 acgacgcggg acaccacctg cgtgtacagc gcctgcgcgg cgcgtccgct cggcggattc
     8521 aatccaccga caaccttcgg aatctgcgcc agcccgtact gtttgtttcc cggatcttcg
     8581 cgttccggcg aaaacgccag gtagaaatcg cacgcgatat tttcattttc cggccccgca
     8641 gcgatggcgc agcgcaaacc gctcttctcc agaatcggca gaaccaattc ttccgttgtc
     8701 cccgggtagg tcgtcgattc gaggaccacc agctgtcccc tttggagatg cggataaatg
     8761 gaaatcgccg tctgctcgac gtagctcaga tccggttcgc ggcgctcatc gagcggcgtg
     8821 ggaacgcaaa tgaggatggc gtcggcatct ttcagcttcg cgaaatcgtt ggtcgcgctg
     8881 aagtgcctgc tgttcacgaa ctgctgaatt ttattttgag gaatgtgctc gatgtaggac
     8941 ttgccggcat taagcatcgt cactttgtga ggatcaacat caaatcccgt gactttgtgc
     9001 cccgcttcgg cgaagcgcag cgctaagggc aggccgacgt agccgcagcc aatgattccc
     9061 accttgagct gagcgccttt gaaaaattcg ggtttccggt cgatgggcat gagttccgtc
     9121 gcactcctct ttgtttctgg ccacgacttg atcggagcca agggattcac gttttcgggg
     9181 ataccgcgtt gcgggcggcc gcacagccgc cagcaggcca cagggccgac gtgacaaaga
     9241 caagtaaata tagcaggttc cgcgcaaaca aaaaaagacg tgcattttcc agtgttacag
     9301 cttgttcaac gcaggttgcc gccagacgcc ggagggtgga agatagtaga gcttgattcg
     9361 aatcatcgag gcgatacgca cagagcagtc cggtcaccat gacattcatc attcttctgc
     9421 tgggacttgg cgttggtgtg ctggtgggcc tcctgggcat cggcgggggc gtagttctcg
     9481 tgccggcaat ggtctatctt ttgcacatgg atcaacacct ggcgcaaggc acttcgcttt
     9541 tcatcttgct gccgcccatc gggctcggag cgctgcggga atactggaag cagggccagg
     9601 tagatttaaa cgccggaatt ttgtgcgcgc tgggaatgct attcggtgcg tatgcgggga
     9661 gcttgatcgc gctgcccatg ccatcacgcc atttaaaagg ggcatttgga tgtttcctga
     9721 tgctggcggc gttcctgctc tggagaaaga cgcaccgaga tacgaacgtg atcggcagcg
     9781 acgaggagaa agctcgtgtc tgatgcgatg ggccgcggcg cgggaatctt cgcaatcgcg
     9841 tgtgtctgcg gagttgcggc gggaatgttt ggcgttggtg gcggcgttct tctggtgccg
     9901 ctcctggggc tgctctttgc gttcagccag catcgcgcgc agggaacttc gttgatcgcg
     9961 ctcataccgc ccacgggagt gctggcgctg atggcctatt cgaaagcagg attcgtctcg
    10021 tggaaaacgg gattgctgct gatccccggc gtgttcttgg gaggcatcgc tggaggaaaa
    10081 gtggccaaat ggatcaagcc gcggatgatg cgccagattt ttgcagggat cttgttcgca
    10141 ctcggcgcgt tgcaagcgtt cagtgcctgg tggcactaag gacggttcat tcgaatggtc
    10201 gatctgcatt gtcacattct gccgggtctc gatgatggcc ccgccaacat ggaagaatct
    10261 atagcgatgg cggaatcggc gatcgccgat ggaatcacgc acgtcgtcgc gacgccgcac
    10321 tccagcaacg aataccattt cggttttaca cacacgcgcc agcttcgaga cgagctgcaa
    10381 gccaaggtcg gcgaccgttt gaaaatcgcc accggctgcg attttcatct caatccggag
    10441 aatctcgcat cgttgctcag ggatgcccgc cagttttgca tcaatcagcg cgattacctg
    10501 ctattggagt tcaacgagtt ttcaattcct ccttcgatgg accagacgtt gcacgagatt
    10561 cagcttgccg gcgtgcagcc cgtgatcacc cacccggagc gcaacggtat tttgcgctcg
    10621 catccggagc gcctgaataa gtgggtgcgc cagggctgct tcgctcaagt gaccggaggc
    10681 gcgctgaccg gaggcttcgg cgccggcgcc cagcaggatg cgctacggtg gatcggcgag
    10741 ggcctaatcc atttcgtggc cagcgacgcg cacaatacgc gaacccggcc gctccgtctg
    10801 caacccgcct acaacgtggt tgtcgatcgt tttggccaag agaaggcgcg cgctttgttc
    10861 ctggataatc cgctcgcggc cttcgagggg cgagagttgc cgcacgttcc agaaatagaa
    10921 gatgaactgt ctccgtcacg ccgcaagcgt tttttctttt tctaataaac agccgcaccc
    10981 tcaaatagaa aaacttccgg gcaactctga tcgtgcggca ctgctacagc cgccttcacc
    11041 gccgagcgat ggtggcggtc gaaaaagcgg gagcaagccc cccgcactcc aaaattcaaa
    11101 ctcactgcgc tgggtgcagg ggcaggcccg cccaggcgtt caacagacca aggagttcgc
    11161 gcactacatc gagagcatcg ctggtggtgc gccaccagtg ccggggatcg aagggatcgc
    11221 cggaggcggc gcgcacgatg gcccggccct ggccgagagc gagcctgcgc cagagcaggc
    11281 gaacgcggtg cgtgtgggct tttgtcgtca caagaatcac ggcgctgtcc ttcctttgcg
    11341 ctagggcagt cgcggcgact ttgatttcat cggcggtatt cacgatggga gtttcgagga
    11401 cgtgaatcgc ttccggcggc actccttcgt gaatcaaaac gagcttgttg taatagtcct
    11461 cgccggagaa gggaataccc atttcttcca gagtttcgcc gggctccgtg gaatgggtta
    11521 gccagatttc cggcgcatac ccttcgcggt aaagtttcgc cgcttcgatg gctcgcaacg
    11581 gcatcgctcc gctgagcacg acgatcgctc tggctttcac aagcgggtcc tccaccacca
    11641 gccagcggcc gacaccgaag aagatcacta cggcaaggac caagccgccg ccaccaacgg
    11701 cgaagagaac gcgccgggtg cgggaacgcg gccggaaaac cgtagcggga ttgtccgggt
    11761 ttgccattgg ggaaccggcg catgctatcc cgccccgccg cggtggtcaa tgcgtggggc
    11821 tcggccagcg ttttacgatg cgcgccgcgc atgctccgtg cccgggcctg cagccaattc
    11881 cgagccgggg caagtggtcg cagcgttatc cggggattgc tgctcgccgg atgaggagct
    11941 gcgagagcgg ctgagacctt ggaccagatt gcgatagaga tcctccgctt ctttctcggc
    12001 tacggcttct ctggccatgg cggtttggct agtgagaagc agttgcgaga tataggcgag
    12061 cgcgatggcg tcgcgacggg cgatgcgctt gcgggcaagc tgtttgacga gattgccgag
    12121 gaagagattc acctcatcgg cagttgtaaa gccatcgatg tcgccgagca ggtcggcggc
    12181 gaggacttcg gggtccacga cgggaacagc cgctttgaga cggtttgcgt ggtaagcgca
    12241 gagcgccggg cgcttagcgc cgttcgagtg acgatgatgc tcgtcgatta gcatgtgaca
    12301 gcggttgcct ttggggctga tgtgctggca atggggatcg atggtggcgc tgactgaggt
    12361 gctggatttg ggagacatgg aaataccttt cgtagggaga ttgaacgtct gaaagcctga
    12421 acgtctgaaa gcctggggct accctccccc cactgttttt gtaaaagatt gacaggacag
    12481 ggtttagggg gtgggggtcg gataaaagat tgtgaggaaa ggagttagag gagagtgtgc
    12541 tagggggata gtggggcagg ggagtagctg aatacgaagc gcttcggcga ggatggcggc
    12601 tgactatcaa aacgtatgtt accacgagag taccgtacaa gtcaagataa atcttgtgat
    12661 gatttggaag tgattgaggg ggaaggggat aggaccgatt gagttgcttg ctgcctttag
    12721 tctgtaactt gattcagggc aacggaggga acatgtccga ttacccgact aacttgggca
    12781 ggtggattgt ttcgagtcgc cgacgattcg ccagaatctc acggtcgcca ctgagaagct
    12841 cgtttctgta tggttgcagg cgcagagacc ctagattaaa tgagaccgca gaatccaaat
    12901 caaaatgcca atcggaatcc ccgccgcagc aatagctgtg aataagatga ttgtgaggaa
    12961 catccaatcc cgccgggtgt tggagccggg ctgctccttc tctcggatca gcagccggta
    13021 gttacgtcta ttcgccttcc aaaagacgtg gaagatgtcg cctgctaccg gtattgctcc
    13081 tagtgtggtt tccagaacta cgttagcaat catgcgaacc agagtgatcg attgcacgcc
    13141 gcgacgccag gccgcgaaca caattaagaa ggatgcgatg cccgccatgg catctccgat
    13201 cccgggtacc catccaatca aggcgtcgag cccgaatcga atgttggttc cagggatccg
    13261 gaacatatcg tccaagagcg cggcaacgta atcgaggtgt tcgtttgacg tgagttggcg
    13321 cggcattggg ccagtcccct taatactata caggcccgcc aaaccggacg ggagagttgc
    13381 tcctatttca ccgaacgcga cttcgggggg aaagcagatg gcgcgcggcc caagcggcga
    13441 aaactcccgt tgttgcaacg acgagcgttt gccccctttg cggtgcgcgg tcgctcttgg
    13501 gcgtactgcg gccggaaggc gatggcggga aacggttcct gctctgttcg ttttgttcgc
    13561 aagagtggga attccggcgg attttgtgcc ccacgtgcgg cgaagaagcc gagaacaagt
    13621 tgccggtata tatcgcggag cagttgccgc atattcgcgt ggaagcctgt gacacctgcc
    13681 agttttatat ccggacggtc gatttgacga aagacggcca cgccatcccg ctagtcgatg
    13741 acttgtccgc gattcccctc acgctctggg cccatgagaa gggctattcg cggctccagc
    13801 caaatctctt gggcacgtaa caacaacggg caacggcaag aaagtcccct tgtccaccgg
    13861 cctcctgggc ttactgcggg attccttcgg caatcacttc ccataaatct ttggcgttca
    13921 cgccggcacg cttgcattcc gcgatcagtt cagaatcaat ggaaagaacg agtggcttcg
    13981 cctgcccaag gcttaagcct agcttcaaga cacttacatc tacggcaccc atgtctgcta
    14041 ccagttcttg gggcatctct agaagcttca agaccccttc gtccatcccc atcgacgaaa
    14101 actcgtcata tacaagtccc caaatggcgg attggccctt tcgctcggtt cggcgtcgta
    14161 tccaggaact gatttcagca acaacatgag gtgtagttgt ctttctctga aagctggcga
    14221 cgaactccga aaggctctgg taagaggaat cgctccgcag atgtcgtaat tcactcttca
    14281 gactttcaaa tcgaagcgtg tgcacggcag aataaagcac aagttcccac aacggcccag
    14341 tgtcgaacac tagaacctga gctccggaga tcgcagggtt aggcggcatt tactgttcaa
    14401 gagaaatacc tggaacgggt gcggcgggaa catcgtaatc ccgagacaaa gacagtcgat
    14461 acccttccaa cgcttgcttg agttttgcgg aaatttcact gaggccatcc ttggaccgat
    14521 gaaggggctg gaattcggga agattcctga aaccaatgag gctgttgcct gcggaattcc
    14581 agaatgcaga gatgacatag tgcgacggca gcgtctgagc gttgttaatc aaatattcgg
    14641 cagccatttt cggaatatcg ttacgcgtat cggttaatga tgctgctcgg ggtcctagcg
    14701 aagctctccc actttcgtag atatcgagcg cagccccaac tgagactaag aattgctccc
    14761 aagaaagcat ccggttgaaa agttcttgtg ccaccgtgtt gagtttcttc agatctgagt
    14821 cgtgtcgctg aaatcccgga ataaaagcag acttggggtg ctcggcgagg aaatccttgt
    14881 agttcggcca ctgagtagaa tccacgtatt cttgtatggt tcggatcatc tcgcagcggc
    14941 ccagatgagg cctccaggtc aaatttccgg aatccaaaag cgaaagctcg cgctgaaggg
    15001 agtcgataat cggatttatg acgacatata tccaggctgc aacgcgccga gattgttttt
    15061 tggggggcat ttccagtcgt ccgctcatga ttcagtagcg tacatcatcg aacagcttaa
    15121 cggaaatggc atattggatg tgtggtttag acacggtaag catgtgagat gccgggtgcc
    15181 ccgggcgccg cgttagcgcc tggggctttt gacttttcca ccagccggtg atgcggattt
    15241 tcaagcacga gcttcacacc ccgcggcgtg tgtcccaaat cttcaaactt tgaaaatcac
    15301 ctcccgctcg ggccctccca ccgccttcca gcatttcccc cgccaccggt gagctctacc
    15361 cctttgtttt cttttattta cgcgcccatt cgaaagacgc cgtaacttgc tcaatataaa
    15421 tactttatta gtacttgaca aatgctataa tacgtggtac catgagtcgt gaatgctcga
    15481 ctccaccaac gccaaagtag ccccctccac taccccgatc attccgatct gtattctcac
    15541 cattccgttc cggcatccga ttctctgcgc aacctctgcg ttctcagcgt ctctgcgtta
    15601 tatttttctt ccgccttttc ttcggcgagt cttcaccagt cacgaatcac cggtcaccag
    15661 tcactaagtc ctttcctttc acatccttgc gcactctttt gcactcatca aaaactcaag
    15721 tcctttattt tcaagcaatt ccgcactctt tgccaaaaac aaccgggggt gggggtaccc
    15781 cccatctctc cacgaggaaa a
//
LOCUS       DASYVO010000034         1668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_128371, whole genome shotgun sequence.
ACCESSION   DASYVO010000034 DASYVO010000000
VERSION     DASYVO010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128371"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VGR03_01265"
     CDS             <1..639
                     /locus_tag="VGR03_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992939.1"
                     /translation="EVVERSAEVLGNLNALSDDVRQLIQGVKEGKGSLGKLLTDEQAY
                     NHLNSLLARGDTMISNIQAGQGTIGKLVTSDEMYTKVNKGLDNVNVILADVRAQKGTI
                     GKLMNDPSLYDQAKQALNNGNAMIDDVRAGKGTLGKLATEDTLYNKLRDTSTNLATAS
                     AKLNDNGTTVGKLFSDPKLYDNLTGMTGDMRLLISDFRKNPKKFLHIKITLF"
     gene            741..1505
                     /gene="tmk"
                     /locus_tag="VGR03_01270"
     CDS             741..1505
                     /gene="tmk"
                     /locus_tag="VGR03_01270"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV1992940.1"
                     /translation="MMENQDVLQKAPRTEPAKSTLPQPGRPVPSRAYPGALIVVEGTD
                     GSGKSTQLYLLKRWLEIGGFRLHFTEWNSSPLVKSATRRGKQRRLLTPTTFSLLHAAD
                     FADRCERQIMPLLQGDYLVLADRYIYTAFARDAARGCPARWLRNLYRFAPIPDITFYF
                     RAPLDVAVNRILSGRPKLKYYEAGMDLGMSYDRAESFRLFQARILEEYDKMVDSDNFV
                     VLDGTLPVNKLQKQMRDIVASKIDLKRFAPRAQESE"
BASE COUNT          404 a          440 c          507 g          317 t
ORIGIN      
        1 gaagtggtgg agaggagcgc ggaggtgctg ggaaacctca atgcgctctc ggacgacgtg
       61 cggcaactga ttcagggcgt gaaggaaggg aagggctcgc tgggaaaact gttaactgac
      121 gagcaggcct acaaccatct caacagttta ctggccaggg gcgacacgat gatttcgaat
      181 attcaggcag gacagggaac gatcgggaag ctggtcacgt cggatgaaat gtacacgaaa
      241 gtgaacaagg gtttggacaa cgtgaatgtg attttggctg atgtgcgcgc gcaaaaaggc
      301 acgatcggaa aattgatgaa tgacccatcg ctgtacgacc aggcaaaaca ggctctcaac
      361 aacggcaatg cgatgatcga cgacgtgcgt gcgggaaagg gcacgcttgg caagctggcg
      421 acggaggaca cgctgtacaa caagctgcgt gataccagca cgaatctggc gacggcctcg
      481 gccaagctga acgacaatgg gaccaccgtg ggtaaactct tctccgatcc aaagttgtat
      541 gacaacctga cgggcatgac cggggatatg cggctgctga taagcgactt ccgcaagaac
      601 ccgaagaaat tcctgcacat caaaatcact cttttctaag acgtctccgg ccgtttggat
      661 gggagaggcc cgctgtgatg taatgcccct gtaataaagc gggattaagc ttccggattg
      721 cacagcttct ctcaaagcga atgatggaaa accaggacgt attacaaaaa gcgccgcgaa
      781 ccgagccggc aaaatccacg cttccgcagc cagggcggcc ggttcccagc cgtgcgtatc
      841 caggcgcgct gattgtggtg gagggaacgg atgggtcggg caagagcaca cagctttatt
      901 tgctaaagcg gtggctggaa attggcggat tccggctgca tttcacggaa tggaattctt
      961 cgccactggt gaagtccgcg acacggcgcg ggaagcagcg ccgcctgctg acgccgacga
     1021 cgttctccct gctccacgcg gcggatttcg cagatcgctg cgaacggcag atcatgcctt
     1081 tattgcaggg cgactacttg gtgctcgcgg atcgctacat ttacacggcg tttgcgcgcg
     1141 atgcggcgcg gggttgcccg gcgcgatggt tgcggaatct gtaccgcttc gcgccgattc
     1201 cggacattac tttttatttt cgcgcgcctc tagacgtggc ggtgaaccgc atcctgagcg
     1261 ggcgtccgaa actgaaatac tatgaagcgg gcatggacct gggaatgtcg tacgaccgcg
     1321 cggagagttt ccgactgttt caagcgcgga ttctggaaga gtacgacaaa atggtcgact
     1381 ccgataattt cgtggtgctg gatggaacgc ttcccgtgaa caaactgcaa aaacagatgc
     1441 gcgacatcgt cgcctccaag atcgatctca agcgcttcgc tccgcgcgct caagaatcgg
     1501 agtaaccatg cccgatcaag aaaaagacaa aaccgccgcc gtggttacgc ccacggagat
     1561 tcctgccccg ccgccggccc cggcgaagcc tgccgcgctg acggaagaag ccggcgcgca
     1621 atactttttc ggagagccgc tcgtgggatt cgatccaagt gaaattac
//
LOCUS       DASYVO010000035         3127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_43434, whole genome shotgun sequence.
ACCESSION   DASYVO010000035 DASYVO010000000
VERSION     DASYVO010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43434"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..990
                     /locus_tag="VGR03_01275"
     CDS             238..990
                     /locus_tag="VGR03_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEV1992941.1"
                     /translation="MPMYMDIHEIPGATAEDVAKAHSADVETQRKYGVEYHKYWFNER
                     SGKIFCLCSAPSPEAAASVHREAHGLLAAKIIEVAPEIAEGFLGGCEVNTAGAALVPG
                     VVGNERDPGIRTVLFTDIVDSIALTQRLGDDAAMVLLQVHDAIVRDALGAWGGREVKH
                     TGDGIMACFLSAVAAARCATRIQRALAQHERENTELPVKVRIGAAAGEPVENHLDLFG
                     STVQLAARLCSVPSRNRAWFRTWLPSCALERT"
     gene            complement(1224..1499)
                     /locus_tag="VGR03_01280"
     CDS             complement(1224..1499)
                     /locus_tag="VGR03_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992942.1"
                     /translation="MTEEKILTKHPLGKSGRNISKHKYETIKQALVGALRNKELTHAE
                     LLSQVNKNLKGKFSDNISWYAETVKLDLEARKIVERTSSKPQKYRLR"
     gene            complement(1514..2239)
                     /locus_tag="VGR03_01285"
     CDS             complement(1514..2239)
                     /locus_tag="VGR03_01285"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEV1992943.1"
                     /translation="MSGRDAQSSDEGPQPALITVDDTHRVSGLLQAPRGARACYVMAH
                     GAGAGMAHPFMGTLANELAKRGIATLRYQFPYMERGSKRPDTPKLAQATVRTAVAEAS
                     RLAPDLALFAGGKSFGGRMTSQAQAASPLSGVRGLVFLGFPLHPAGRPSDERAAHLFD
                     VQIPMLFMQGTRDQLADLPLLQTLVGQLGTRATLKLVQDADHSFHVPARTGRKDSQVM
                     AQVSEALADWIELAIPRTAGKKG"
     gene            complement(2236..2595)
                     /locus_tag="VGR03_01290"
     CDS             complement(2236..2595)
                     /locus_tag="VGR03_01290"
                     /inference="COORDINATES: protein motif:HMM:NF018893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEV1992944.1"
                     /translation="MSYVDGFVVPVPRKNLAAYRRMAQKAGKVWKEYGALEYKEWISD
                     DVKVGKLTSFPRSVKLKPGETVVFSYIVYKSRAHRDRVNAKVMKDPRLADMMDPKSMP
                     FDGKRLIYGGFKKLVFV"
     gene            complement(2655..>3127)
                     /locus_tag="VGR03_01295"
     CDS             complement(2655..>3127)
                     /locus_tag="VGR03_01295"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV1992945.1"
                     /translation="ATTMPDDFRVQRTTSIKAPPEKIFPLINDLHRWGSWSPWEKMDP
                     EMKRTYSGAAAGKGAAYAWQGNRKVGEGRMEIVDTSPPSKITIKLDFIKPFEGHNTAE
                     FTLEPKGDSTNITWAMYGPSVYMAKVMGVFVSMDKMIGKEFETGLANLKSAAET"
BASE COUNT          600 a          920 c          929 g          678 t
ORIGIN      
        1 aagacgcagg cgatggaggc gtacgaggcg gcgcttgccg cagatccaag catggccgaa
       61 tgccactaca acctggcgct gctgtgcgaa aaactcgaga agccgaagga cgcgatccgg
      121 cacatggcac ggtatcgcgc attgatcggg ccccgatcga agtaaaacgc gcgttcaacc
      181 cggcccggcg ggccggttaa catagacgtc gagacgtcac gctttcgaaa gatcgacatg
      241 ccgatgtaca tggacatcca cgagatcccc ggagctaccg cggaagacgt tgcgaaggcc
      301 cacagcgcgg atgtcgaaac gcagcggaag tatggcgtcg agtatcacaa atactggttc
      361 aacgagcgca gcggcaagat tttctgcctc tgcagcgcac caagtcccga ggcggcagcc
      421 tccgtgcatc gtgaagcgca tggcctgctc gcggcgaaga ttatcgaagt cgcgcccgag
      481 attgcggagg gattcctggg gggatgtgaa gtgaatactg cgggcgcggc gctcgttcct
      541 ggcgtcgttg gaaacgagcg cgacccggga atccggaccg ttctgttcac cgatatcgta
      601 gactccatcg cgctcacgca acgccttggg gacgacgcgg ccatggttct tcttcaggtc
      661 catgatgcga tcgtccgaga cgccctgggc gcgtggggcg gtcgcgaggt caagcacacc
      721 ggagacggca tcatggcgtg cttcctgtcg gctgtggcgg cggctcgctg cgcgaccagg
      781 atccagcgtg cgttggctca acacgagcgt gagaacacag agctcccggt caaggttcgg
      841 atcggcgccg cggcgggaga gcccgtcgag aatcacctgg atctcttcgg atcgacggtt
      901 cagcttgccg cgcggctctg ttccgtgccc agccggaaca gagcctggtt tcgaacgtgg
      961 ttgccgagct gtgcattgga aaggacttga ccttccagga cctcggagaa gtttcactca
     1021 agggcttcga tcgcccgatc agagtgcacg ctatcaaaca gcccggagaa gcggcgccgc
     1081 actgaagagt caaatcggcg ccaatcctct gcgtcggatc tacatatcga cgtgtgaatc
     1141 gcgaagagcc gcaccgggcc gataacagct tcggaatttg cacgtcgaag cgatgcgctg
     1201 cggcgatcgt ctttactctc ggactatctc agccgatatt tttgaggttt tgaggaagtt
     1261 cgttccacta tttttctagc ttccagatcc agcttgacgg tctctgcata ccaacttatg
     1321 ttgtctgaaa atttgccctt caggtttttg ttgacctgac tgaggagttc tgcatgagtt
     1381 agctctttat ttcgcaaagc tcctacgaga gcctgcttga tcgtttcata cttatgcttg
     1441 ctgatgtttc gtccactttt gcccaatggg tgtttggtga gtattttttc ttcggtcatt
     1501 tctcctccat gaatcagcct ttctttccgg cggtgcgcgg gatcgcaagt tcgatccaat
     1561 ccgccaatgc ttccgacact tgggccatga cttgcgaatc cttgcgtccc gtgcgcgccg
     1621 gaacgtgaaa cgaatgatcg gcatcctgga cgagcttgag tgttgcgcgc gtcccgagct
     1681 gtccgacgag cgtttgcagc agcggcaagt ccgcgagctg atcgcgagtt ccctgcatga
     1741 acagcatcgg gatttgcacg tcgaagaggt gagcagcgcg ctcgtccgac ggccgcccgg
     1801 ccggatggag cgggaatccg aggaatacca ggccgcggac tccggacaag ggcgatgcgg
     1861 cctgcgcctg tgaggtcatg cggccgccaa acgatttccc gcccgcgaaa agggcaaggt
     1921 ccggtgccag acgagacgct tccgccactg ctgtgcggac cgtggcttgg gcaagcttgg
     1981 gagtgtccgg ccgcttcgac ccgcgctcca tgtagggaaa ctgatagcga agcgtggcga
     2041 tgccgcgctt cgcgagttcg ttcgcgagtg tccccatgaa agggtgcgcc atacccgcgc
     2101 ctgcaccgtg cgccatgacg tagcaagcgc gtgccccgcg cggtgcttgc agcaaaccgg
     2161 agacgcgatg ggtgtcgtcg acagttatca gcgcaggctg cggaccctca tcactcgact
     2221 gcgcgtcccg accgctcaaa cgaagaccag tttcttgaat ccgccgtaga tcaggcgctt
     2281 gccgtcgaaa ggcatcgact tcggatccat catgtcggcc agccgcggat ccttcatgac
     2341 tttggcgttc acccgatcgc gatgcgcgcg cgacttgtac acgatgtacg agaagacgac
     2401 cgtttcgccc ggcttgagct tgacgctccg tgggaacgac gtgagcttgc cgaccttcac
     2461 gtcgtcgctg atccactctt tgtattcgag ggcgccatat tccttccaca cttttcctgc
     2521 cttctgggcc atgcgccgat aggccgccag gtttttccgc ggtaccggta ccacaaagcc
     2581 atcaacatag ctcatatcgc ctccaggttt aacgaggttt tacagcaatt gcaagggtgc
     2641 cgcgtgtatg ccgctcaggt ttcagcggcg ctcttcaggt tggcaagacc ggtctcgaat
     2701 tccttgccta tcatcttgtc catgctgacg aatacgccca tgactttcgc catgtagacg
     2761 ctcgggccat acatggccca ggtgatgttc gtggaatcgc cctttggctc gagcgtaaat
     2821 tcagcagtgt tatggccttc gaacggcttg atgaaatcca gcttgatcgt gatcttggat
     2881 ggcggcgatg tatcgacgat ctccatgcgc ccctcgccga ccttcctgtt accttgccac
     2941 gcatacgccg cacccttgcc ggctgccgcg ccgctgtagg ttctcttcat ttcgggatcc
     3001 attttctccc atggcgacca ggaaccccaa cgatggaggt cgttgatgag gggaaagatt
     3061 ttctccggcg gcgctttgat gctcgtcgta cgctggacac ggaagtcatc aggcatggtc
     3121 gtcgcat
//
LOCUS       DASYVO010000036         2973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_85234, whole genome shotgun sequence.
ACCESSION   DASYVO010000036 DASYVO010000000
VERSION     DASYVO010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85234"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VGR03_01300"
     CDS             complement(<1..250)
                     /locus_tag="VGR03_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV1992946.1"
                     /translation="MKLPPFFEEDRLAVDAALERLLPVGTAQPSSIHTAMRYSVFAGG
                     KRIRPILCLETARIFAGDVAPAFHPACAIEFIHTYSLIH"
     gene            complement(291..563)
                     /locus_tag="VGR03_01305"
     CDS             complement(291..563)
                     /locus_tag="VGR03_01305"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV1992947.1"
                     /translation="MNSPAPKKPEPPKKPDFERSLARLEEVVRRLESPQLSLDDAMKL
                     FEEGVALSRECQKQLEEAEGRVEILLKKADGKLAAEPFDPERESES"
     gene            complement(592..1431)
                     /gene="mutM"
                     /locus_tag="VGR03_01310"
     CDS             complement(592..1431)
                     /gene="mutM"
                     /locus_tag="VGR03_01310"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEV1992948.1"
                     /translation="MPELPEVEAVVRTLRPLVEGRRIRCVHVFHPIATKPQAASNVAR
                     HAQGSRIRIVDRKGKYVLLVLDRGLLTMHFRLDGQLLWFANGKELLQRANQRENGVHV
                     DVAFELDKGVLGFADRRHFGRVHAWESADDCLGLNALGIDALSRDFTSRCFAELLATS
                     KRPLKEFLLDQTRLAGIGNIYSCESLWHARLDPRRRANTLKPQEARRLHKAIVSVLAR
                     ALECCLHPAPNFRDPEWWFQGLENILRAYGREGKPCRRCGEPIRRIAQGGRSTYYCRR
                     CQK"
     gene            complement(1447..>2973)
                     /locus_tag="VGR03_01315"
     CDS             complement(1447..>2973)
                     /locus_tag="VGR03_01315"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEV1992949.1"
                     /translation="NDTHLELSIPPIWYEIHLTAPGWNVKGFTLPGAPMIIIGHNDRI
                     TWGFTNNGADVQDLYVEIFNPASPDEYRVKGAWTKSQIIDETIHIKGQPDEHLKVTVT
                     RHGPVVHREGEKAYALRWTATEPGGLANSYNWLGKAHNWKEFREIMKRVWGPGQNAVY
                     ADVEGNIGYVMAARVPIRKKGHGEVPVPGDTDEYEWTGYIPFEQLPQALNPESGLIVT
                     ANARVVGPNYKPYLTDRWEEPYRTARIYDLLHDRHDLRPEDMLKVQTDTYSYPHVFLA
                     EQLSAAAKTLKPKDARSQKLIEGLKDWNGIADADSAEVSFLHAARRAAIDILLEPFLG
                     NDTNLYQWRSNAFLQKILTDRPAKWLPGAYKNYDEMLAAAADIAVTKLAEQSKSERVE
                     DWAWKRFNSLDMFHPIGREGLLKRFLSITDKPQSGTLYSVRAPTRTHGPAMRFVGNPG
                     NWDESILLIPTGQSGQPGSSHYSDQFSYWYEGKPIFAAFSDAAEANARKHTLALKPGS
                     "
BASE COUNT          574 a          829 c          934 g          636 t
ORIGIN      
        1 cgtggatcaa ggagtaggtg tggatgaatt cgatggcgca cgcggggtga aacgcgggag
       61 cgacgtcgcc ggcgaagatg cgcgccgtct cgagacagag gatgggacgg attcgctttc
      121 caccggcgaa gacgctgtaa cgcatggccg tatgaatgga agagggttgc gccgttccga
      181 ccggcagcag ccgctcgaga gcagcatcga cggccaggcg gtcttcttcg aagaatggag
      241 gaagtttcat ctctgcaggg aattggcgcg cagcgggctg acgtggcgcg tcacgattcg
      301 ctctcccgct cgggatcgaa cggctcggcg gcaagcttgc catcggcttt cttgaggagg
      361 atttccacgc gcccctccgc ctcttccagt tgcttctggc actcgcgcga aagtgcgacg
      421 ccctcttcga acagcttcat cgcgtcatcc aaagagagct gcgggctttc caggcggcgg
      481 acgacttcct caaggcgggc tagcgagcgc tcaaaatcgg gctttttcgg cggttcgggt
      541 ttcttgggag caggtgaatt cacgcgatcg gcccgtatcc tttagaggcg cctatttctg
      601 acaacgcctg caatagtaag ttgaccggcc gccctgcgca atgcgccgga tcggctctcc
      661 gcagcggcgg cagggttttc cctcgcggcc gtaggcgcga agaatgtttt ctaacccctg
      721 gaaccaccac tccggatcgc gaaagttggg cgccggatgc agacagcatt ctaaggcacg
      781 cgcgagaacg gacacaattg ctttgtgcaa acggcgcgct tcctgcggtt tcagggtgtt
      841 ggcgcggcgg cgagggtcga ggcgagcgtg ccacagcgat tcgcaagagt agatgttgcc
      901 aatccccgcg aggcgcgttt gatccagcaa gaattccttc aaggggcgct ttgacgtggc
      961 gagcaattcg gcaaagcagc gggaagtgaa atcgcgcgag agggcgtcga tgcccagcgc
     1021 gttgagccca aggcaatcat cggcggattc ccaggcgtga accctgccga agtggcggcg
     1081 atcggcgaag ccgaggacac ctttgtccag ctcgaaggca acatcaacgt gcacgccgtt
     1141 ttcgcgctgg ttggcgcgtt gcagcagttc tttgccattt gcaaaccaca ggagctggcc
     1201 atcgagccga aaatgcatcg tgagtaggcc acggtccaag acgagaagca catatttgcc
     1261 tttgcgatcg acgatgcgaa tgcggctgcc ttgggcatgg cgagcaacat tcgaagcggc
     1321 ttgcggcttc gtggcgatgg ggtgaaagac gtgaacgcaa cggattcgcc ggccctccac
     1381 aagaggacga agagtgcgca caacggcttc gacctccggg agttcaggca tgatcgctat
     1441 acgagatcag gaaccaggct taagagcgag tgtatgcttt cgcgcgtttg cctcggcagc
     1501 gtcgctgaac gctgcgaaaa tgggtttgcc ttcgtaccag taggaaaact gatcggagta
     1561 atggctgctg cccggctggc cggattgtcc cgtggggatg agcagaatgg attcatccca
     1621 gtttccgggg ttgccgacaa agcgcatcgc ggggccatga gttcttgtgg gcgcgcgcac
     1681 gctgtagagc gttccggatt ggggtttgtc cgtgatgcta agaaatcgct tcaagagacc
     1741 ctcgcgcccg atgggatgga acatgtcgag ggaattaaag cgcttccacg cccaatcttc
     1801 aacgcgttcg ctcttgctct gttcggccag cttggtcaca gcgatgtcgg cagccgcggc
     1861 gagcatttcg tcatagtttt tataagcacc gggcagccac ttcgcgggcc gatccgtaag
     1921 gattttttgc agaaacgcat tgctccgcca ctgataaaga ttcgtgtcat ttccaaggaa
     1981 cggctcgagg aggatgtcga tggccgcccg gcgcgccgcg tgcagaaaag aaacttccgc
     2041 agagtcggca tcggcgattc cgttccaatc cttcaggcct tcgatgagct tttgagagcg
     2101 tgcgtctttg ggcttcagag ttttcgcggc cgccgataat tgctccgcga ggaagacatg
     2161 cggataactg tacgtgtcgg tttggacttt cagcatgtcc tcgggccgca agtcgtggcg
     2221 gtcatgcagc agatcgtaga tgcgcgcggt gcgatacggc tcttcccagc gatccgtgag
     2281 atacggtttg taatttggcc ccaccacacg cgcattggcg gtgacaatca atccactttc
     2341 agggttgagc gcctgcggca gttgctcgaa cggaatgtag ccggtccact cgtactcgtc
     2401 tgtgtcgccg ggaacgggta cttcgccgtg gcccttcttt cgaatcggca ctcgcgcggc
     2461 catcacgtat ccgatattgc cttcgacatc cgcgtaaacc gcgttctgcc ccgggcccca
     2521 cacgcgcttc atgatttcgc ggaattcttt ccagttgtgc gccttgccca gccagttgta
     2581 gctgttggcg agcccacccg gttcagtcgc ggtccagcgc agtgcgtaag ctttttctcc
     2641 ttcgcgatga acgaccgggc cgtggcgcgt cacggtaact ttcagatgtt cgtccggctg
     2701 acctttgatg tgaatcgtct cgtcgatgat ctgcgacttc gtccacgcgc cctttacccg
     2761 atactcatcc ggcgaagccg gattgaatat ctcgacgtaa agatcctgaa cgtcggcccc
     2821 gttgttggtg aagccccagg tgatgcggtc gttgtggccg atgatgatca tgggcgcacc
     2881 gggcagagtg aagcctttaa cgttccagcc gggcgcggtg agatgaatct cgtaccagat
     2941 tggcgggatg gaaagctcca gatgggtgtc gtt
//
LOCUS       DASYVO010000037         1777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_646, whole genome shotgun sequence.
ACCESSION   DASYVO010000037 DASYVO010000000
VERSION     DASYVO010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VGR03_01320"
     CDS             complement(<1..243)
                     /locus_tag="VGR03_01320"
                     /inference="COORDINATES: protein motif:HMM:NF022598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2924 domain-containing protein"
                     /protein_id="HEV1992950.1"
                     /translation="MPMQTHTQEPVAAELAILPGCSIHELRERWRALFRAKPPAAFGP
                     DLLRRSIAQKLQDDTYGKLSSAVQRELNRIVATLERK"
     gene            complement(365..1159)
                     /locus_tag="VGR03_01325"
     CDS             complement(365..1159)
                     /locus_tag="VGR03_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992951.1"
                     /translation="MRELRDEPWARPLLDDINATGGLTRDNKTKLFELRFGYALRHAG
                     ITPRYEVAGEAQSTLDFGFNSAKKDWLVELMRLEETQAAKGATRSETDEHGTPWVKRV
                     LATDAEDPKRSEEGETLKAVERICQKCEHDGRPHKFPAPAGAHHAILVDIRTFLNGGD
                     EYDRVHVGLGGEYVPDEMCRRYWDKQLISGVFSPRTTLRGAAEARSRVHFLGFVREQA
                     YAAGAFGKAIQFIANPNLLKTEAEVRAAIATWPLQPAAILNVGRGK"
     gene            complement(1297..>1777)
                     /gene="terL"
                     /locus_tag="VGR03_01330"
     CDS             complement(1297..>1777)
                     /gene="terL"
                     /locus_tag="VGR03_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEV1992952.1"
                     /translation="PATIRWGPHPKLESRRKLQHNPFSNGLLDVFRERLNYPDLRRAA
                     KKLAERYDANTILIEDKASGTQLIQELQHEGMFGVTPYEPPPGTDKIMRLHSQTAMFE
                     NGFVLLPRTAPWLPDYLHELTGFPGTKHDDQIDSTTQALDYLRSAYSPLEIWERLAK"
BASE COUNT          327 a          520 c          541 g          389 t
ORIGIN      
        1 cttcctctcc agggtcgcga cgattcgatt aagttcgcgt tgcacggctg aggacagctt
       61 gccgtaggtg tcatcctgca gtttctgagc gatgctgcgg cggagaagat ccgggccgaa
      121 agctgccggt ggtttagcgc gaaagagtgc gcgccatcgc tcgcgcaatt cgtgaatcga
      181 acatccgggc aatatcgcga gctcggccgc aaccggctcc tgggtatggg tctgcatagg
      241 catcgagcgg ctccggcagc ggaatctgcc gtccatgcat gctctgggtg gcctgcaagt
      301 ccagtggaaa ggctcgtaat taagtctatg aaatcaaaca attaggctag tttcgaaccg
      361 ccttctattt tccgcgcccg acgttcagga tcgcggctgg ctgcaatggc cacgtcgcga
      421 ttgcggcgcg aacctctgct tcggtcttga ggagattcgg attggcgatg aactggatcg
      481 ctttcccaaa cgcgccggcg gcatatgcct gctcgcgcac gaagccaagg aaatggacgc
      541 gactgcgcgc ttccgcagcg ccgcgaaggg tggtgcgcgg gctgaacacc cctgaaatca
      601 gctgcttgtc ccagtaccga cgacacattt cgtccggaac gtattcgccg ccgagcccga
      661 cgtgaacacg gtcgtattca tcgccgccgt tcagaaaggt gcggatatcg acgagaatag
      721 cgtgatgtgc accggctggc gctgggaact tatgaggcct gccgtcgtgc tcgcatttct
      781 gacagatgcg ctcgaccgcc ttgagcgttt ccccttcctc tgaccgcttc gggtcttcgg
      841 cgtcagtggc gagcacgcgt ttgacccacg gggttccgtg ttcgtcagtt tccgaccgcg
      901 ttgccccctt cgcggcttgc gtttcctcca gccgcatcag ttcaacgagc caatcctttt
      961 tcgcggagtt gaagccgaaa tccagtgttg attgcgcttc ccccgcgacc tcgtaacgcg
     1021 gcgtgatccc ggcatggcgc agcgcgtagc cgaagcgcag ctcgaaaagc ttcgtcttgt
     1081 tatcacgcgt caggccgccg gtggcgttga tgtcatccag aagcggccgc gcccacggct
     1141 catcccgcag ctcgcgcatg atcgcttccg agaacgccgc ttcgtctccc gaaagattgt
     1201 cccagaaatg ttccgcaacc ttcgtcatcg caaccattct aacacatcgg cgcgcaactg
     1261 gctcccgggt gtgggtctgc agaccaccag ggaacgtcat ttcgctaagc gttcccagat
     1321 ctcaagcggc gagtacgccg accgcaggta gtccagcgcc tgtgtagtgg agtcgatctg
     1381 gtcgtcgtgc ttggttccgg ggaagccggt caattcgtgc agatagtcgg gaagccatgg
     1441 cgctgtacgc ggaagcagca cgaagccgtt ctcgaacatc gcggtctggg agtgcaagcg
     1501 catgatcttg tctgtgccgg gagggggctc atacggtgtg acgccgaaca ttccttcgtg
     1561 ctggagctcc tgaatcagct gcgttcctga agctttgtcc tcgatcagga tggtattggc
     1621 atcgtagcgc tccgccagtt tcttagcggc gcgccggagg tcgggatagt tcaggcgttc
     1681 gcgaaaaaca tctaagagac cgtttgagaa tggattgtgt tgaagctttc ggcgtgattc
     1741 aagcttggga tgtggacccc agcgaatcgt ggccgga
//
LOCUS       DASYVO010000038         8486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_269374, whole genome shotgun sequence.
ACCESSION   DASYVO010000038 DASYVO010000000
VERSION     DASYVO010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269374"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VGR03_01335"
     CDS             complement(<1..193)
                     /locus_tag="VGR03_01335"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEV1992953.1"
                     /translation="MALALLLVSLPLPAQNFTTPEYRAKASYLAKFPSFVEWPERALP
                     PRSAPFLLCVFGEYPFGVAL"
     gene            complement(477..553)
                     /locus_tag="VGR03_01340"
     tRNA            complement(477..553)
                     /locus_tag="VGR03_01340"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Cys,seq:gca)
     gene            complement(624..1646)
                     /gene="galT"
                     /locus_tag="VGR03_01345"
     CDS             complement(624..1646)
                     /gene="galT"
                     /locus_tag="VGR03_01345"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEV1992954.1"
                     /translation="MPELRKDPITGRWVIISTERGKRPNDFLRESVVAKDAKNCPFCA
                     GNEAKTPPEVLVYGRNGGGANTPGWSIRVVPNKFPALGIEGDLDREGEGLFDKMNGVG
                     AHEVIVESPDHAATLATLPERAVEDVLWGYRDRMLDLKNDKRFRYILIFKNHGEAAGA
                     TVDHPHSQLIALPIVPRRVREEVDNCWHYYDEKERCIFCDIIRQERDTGERVIGENEH
                     FITLAPYAPRFPFEMWLLPKAHGSSYENNQSSMYASLARMLKDTLMRLDEVLDRPALN
                     FMIHTSPIGEEINDHYHWHIEIIPKLTKVAGFEWGTGFYINPTPPEESARFLREAKIP
                     TIAEKK"
     gene            complement(1756..2229)
                     /locus_tag="VGR03_01350"
     CDS             complement(1756..2229)
                     /locus_tag="VGR03_01350"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEV1992955.1"
                     /translation="MPIFSQLMFRAVHWAARRGLREEESFSSDAKKQEARRTGVRGET
                     YAYWYLRRQGYVFVARNYTPRGIKGEIDLVGYDGKTLAFVEVRTRTIREDLPALPELS
                     VTREKQHVVVRTAQRFLAERHVGECPCRFDVMAIDNCPGQPPVVRLHKDAFSPQR"
     gene            complement(2366..3715)
                     /locus_tag="VGR03_01355"
     CDS             complement(2366..3715)
                     /locus_tag="VGR03_01355"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HEV1992956.1"
                     /translation="MPTTLELKTPRLVTALPGPNAKRVLEQDAKFMSPSYTRDYPLVA
                     KRGHGAIVEDVDGNAFLDFAAGIAVCSTGHCHPKVVSAIQKQAAELIHMSGTDFYYES
                     MPQLAERLGRTMPGAEDKKVFYTNSGTEAIEGAIKLARYATKRDKLIAFYGGFHGRSM
                     GALSLTASKATQRKGFGALLSGIEHIPYPYAYRCAHGHTAETCGAEILEQLEGQIFKR
                     LFDPEDVAGIIIEPIQGEGGYVPAPKFFLQELQHICRQHGIMFIVDEVQSGMGRTGKW
                     WACEHAGIEPDILCSAKGIASGMPLGAIIARESVMRWKPGSHGTTFGGNPVCIAAALA
                     TMDLIESEYMENARRMGDYLFGRLADWTKNFKIVGDVRGRGLMIGIEIVRDQRTKEKA
                     ADLRNAIVDMAFHKGLLVLGAGENTIRLSPPLLIDREQADFAARTLEDCFRKAEKKI"
     gene            complement(3918..5357)
                     /gene="cysS"
                     /locus_tag="VGR03_01360"
     CDS             complement(3918..5357)
                     /gene="cysS"
                     /locus_tag="VGR03_01360"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV1992957.1"
                     /translation="MAELQLHNTLSGKIENFVPQKAGEVRMYTCGPTVYDYAHIGNYR
                     TFVFQDILRRFLKLRGFKLNHVMNLTDVDDRIIANAAAAGKSIRDYTEKFVQAFFEDC
                     KTLSIEAPEHWIRATDHIEEMVKLIQRLQEKLFTYPGEGSIYYRIAKFPEYGKLSKID
                     LTGIQVGARVDNDRYEKENARDFALWKAPKPGEHFWETSIGPGRPGWHIECSAMAMKY
                     LGETLDIHTGGIDLAFPHHENEIAQSEGATGKPFARYWLHAEHLLVEGEKMSKSQGNF
                     FTLRDLFAKGYKPSALRFALASVPYRKQLNFTFDGLQQAASSVERLRNFADRLKRGKF
                     PTGRQEGMAARIAKAAEEFDAGLADDLNTARALAAVFDLVREANIAVDKGEFRQGDVP
                     AVLEFLATFDKVFAVVEDNDADKLRTLGYGSAESGPGDEEIDRLVAERNAAKKKRDFA
                     TADRIRKELADRGIIIEDAKDGSVRWKRK"
     gene            5546..7540
                     /locus_tag="VGR03_01365"
     CDS             5546..7540
                     /locus_tag="VGR03_01365"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV1992958.1"
                     /translation="MNRSLSAGALFALAAFATPILAQEPPKASAPKLLTPEASLDLRS
                     ISDLQFSPDGNRLAFVVTEPAKGEHRARHIWIYEKPSGAIRQFTYSAKSDFLPRWSPD
                     GKQLAFLSDRDEQQQVYAMRANGGEASALTKGKRGVKDFAWSPDGKQIAFLAPDAKTE
                     AEEKKEKDKDDAHVADKEDKHARLWLLTLDTGEAQALTEPKWEISEAAWHPSGTGLML
                     SATDHPESDQNTEHIFFFRLWDTANGDHKAVDTMTQVLAPRGHFGNVRLAADGLRMAF
                     VGCREDGPTPHDLMLAHHGDKATQNLSGASVDRPVFDYRWSKDGGLLAVVEDGFHTKF
                     VAFTAEGALKDVPAIPANPNAFGVSDSGEVVFAGQTATTPQELWLWDQKSAPKQISHL
                     NDSWKQYTLSTPEFYKYKSFDGMEIEAALLKPLGHDGKSKLPLVALIHGGPTGAWQDS
                     IETWGQLLAARGYAVFYPNIRGSSGYGQKFIEMNRGDWGGGDYRDVMAGVDDLVARGI
                     ADPNKLAIGGWSYGGYMSEWAITQTTRFKAAVSGAGLSNLISEYGTEQGPSYDEWFYG
                     VPYEPEKIAGFLNSSPFVHLKNVKTPTLILQGDADPVDPPGQSQELYRGLKRYGVEAE
                     LVVYPREPHGFHEEKHLLDRLNRILAWYDKHLKEGSPATR"
     gene            7898..>8486
                     /locus_tag="VGR03_01370"
     CDS             7898..>8486
                     /locus_tag="VGR03_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1992959.1"
                     /translation="MLKLRKVEIVGFKSFFERTVVTFSGSGTTCIVGPNGCGKSNVVD
                     AISWVLGEQSHKSLRAERMADCIFNGTTKRPPLGLAEVTITMEDAELAEAARFVMESA
                     AADSATDSAALVTENSETSGLTDVTEILSLDPAQAPETQEAAADGSAPSPAEPGGKFK
                     RKKKTADKPVLAMKPGEVVVSRRLYRSGQSEYLING"
BASE COUNT         1740 a         2581 c         2365 g         1800 t
ORIGIN      
        1 ctagcgcgac tccaaaggga tattcgccaa aaacgcagag tagaaagggc gcgctcctcg
       61 gcggcagagc acgttccggc cactcgacaa aactgggaaa cttcgccagg tagctggcct
      121 tcgcgcgata ttccggagtg gtgaagttct gcgccgggag gggcagtgaa accaaaagga
      181 gagccagtgc cacacccaag cgcaacagcg cccgtgacag cagtctcggt gcggtcgaca
      241 aatatcgaag catgcactga aggcgaaaca cgtgaatccc cccaagaaca agggcacgcg
      301 agtgtccacg tgtaagcgcg gccatctcgc taggtgattg ataagaatca agttcggctt
      361 gaggtctttt gtcctgttag gggcgaacta tccgtggtgg aacagaatta gaaaagaggt
      421 tcaatttgaa actatcggct gaatcgcctg atttacgaga ggaaaataaa gaagtttgga
      481 ggcgcgggtg ggattcgaac ccacgaatgg aggttttgca gacctctccc ttgggccact
      541 tgggtaccgc gccgaatctt taagtatagc aaatctgttt gaactcttcg gacatcccga
      601 agcagcgttg cttccgccta cccctacttt ttctccgcga tggtgggtat ctttgcttca
      661 cgcaaaaatc gcgccgattc ttccggaggt gttggattaa tgtaaaagcc cgtgccccac
      721 tcaaagccgg ccactttcgt caactttgga atgatctcga tatgccagtg gtagtgatcg
      781 ttgatctctt caccaatcgg agatgtgtga atcatgaaat tgagggcggg ccggtccagc
      841 acctcgtcca gccgcatcag cgtatccttc agcatccgag ccagacttgc atacatggag
      901 ctctggttat tctcgtacga tgatccgtgc gcctttggca gcagccacat ttcaaacggg
      961 aagcgaggcg cataaggtgc cagcgtgatg aagtgttcgt tttcaccgat tacgcgctcg
     1021 ccggtgtcac gttcctgccg gataatgtcg cagaagatac agcgctcctt ctcgtcgtaa
     1081 tagtgccagc agttgtcgac ttcttcgcgc acccgccgcg ggacgattgg cagcgcgatc
     1141 aattgagaat gcggatgatc gacggtagcg ccggcagctt caccgtggtt cttgaagatc
     1201 agaatgtacc gaaagcgttt gtcattcttc aggtccagca tgcggtcccg gtatccccag
     1261 agcacgtctt ccaccgcgcg ctccggcaga gtcgccagcg tcgccgcatg gtcgggcgac
     1321 tccacgatca cttcatgagc gccgactccg ttcatcttgt cgaagaggcc ttccccttcg
     1381 cggtccagat cgccttcgat gcctagcgct gggaacttgt ttggcaccac gcgaatgctc
     1441 caacccggtg tgttggcgcc gccgccgttc cttccatata ccagaacctc aggcggcgtt
     1501 ttggcttcat tgccggcgca gaaggggcaa ttcttcgcgt ccttggcgac aacactctcc
     1561 cgcaggaaat cgttcggccg ctttcctcgt tccgtcgaaa tgatcaccca gcggcccgtg
     1621 atcgggtcct tccgtaactc cggcaaagca atcctcctcg aataggttct gggtcacccg
     1681 cgcagggtga gcaacccgga gactttgaat tgtaccgcgt ctggcatttc cgtgaagatg
     1741 ttccagttac gctcctcacc tttgagggct gaacgcatcc ttgtgcaacc gtaccaccgg
     1801 cggctgcccc ggacagttat caatcgccat tacatcaaac cggcagggac attcgccgac
     1861 gtgacgttcc gccagaaatc gctgcgccgt gcgaaccacc acgtgttgtt tttctctggt
     1921 aacgctaagt tccggcagcg ccggcaagtc ctcccggatg gtgcgcgttc tgacttccac
     1981 aaatgccagc gtcttgccgt cgtaaccgac gagatcgatt tcgcctttga tgccgcgcgg
     2041 agtgtaattc cgggctacga atacgtatcc ctgcctccgc aaataccagt aggcgtaggt
     2101 ttcccctcga acgccggtgc gccgggcttc ctgctttttc gcatcgctgg aaaaactctc
     2161 ctcttcgcgc aggccgcgcc gcgccgccca gtgcaccgcc cgaaacatca gttgtgaaaa
     2221 tatcggcatg ctcccggcac cgattccccc cagcctcccg cgcagtataa acaaaaatcc
     2281 cgtggctgcc atagttcttc tgcggcagcc acgggaccct ttctggttct tctctgagaa
     2341 ctctgcgtcc tcagtgatct ctgtgttaga tctttttctc cgctttgcgg aagcaatctt
     2401 ccagcgtccg cgcggcaaag tccgcctgtt cccgatcgat caggagcggc ggcgacaggc
     2461 gaatggtgtt ctcaccagcg ccgagcacca gcaggccctt gtgaaacgcc atatcgacga
     2521 tcgcgttgcg cagatccgcg gccttttcct tggtccgctg atcccgcacg atctcaatgc
     2581 cgatcatcaa tccgcgtcca cgtacatcgc cgacgatttt gaagttcttc gtccaatcgg
     2641 ccaggcgccc gaagaggtag tcgcccatgc gacgggcgtt ttccatgtac tcgctctcga
     2701 tcaggtccat ggtggccagc gccgctgcaa tgcaaaccgg gtttccgccg aaggtcgtgc
     2761 cgtgcgagcc cggcttccaa cgcatcacgc tttctcttgc gatgatcgcg ccgagcggca
     2821 tgccgctcgc gatgcccttc gcggaacaga gaatgtccgg ttcgattccg gcgtgctcgc
     2881 aggcccacca cttcccggta cgtcccatgc cggactgcac ttcgtccacg atgaacatga
     2941 tgccgtgctg gcgacagatg tgctgcaatt cctgcaggaa aaactttggc gccggcacat
     3001 agccgccttc gccctgaatg ggctcgatga tgatgcccgc gacgtcttcg ggatcgaaca
     3061 gccgcttgaa aatttgccct tcgagctgtt cgaggatttc cgcgccgcag gtctccgcgg
     3121 tgtgaccgtg cgcacagcgg taagcgtacg gataggggat gtgctcgatg ccactgagca
     3181 gcgcgccgaa gcccttgcgc tgcgtcgctt tcgacgccgt caacgaaaga gcgcccatcg
     3241 agcggccgtg gaagccgccg taaaaagcga tgagcttatc gcgcttcgtg gcgtatctcg
     3301 ccagtttgat ggcgccttcg atcgcttcgg tcccggaatt cgtgtagaag accttcttgt
     3361 cctcagcgcc gggcatcgtg cggcccagcc gctctgccag ttgcggcatg ctttcgtaat
     3421 aaaaatccgt gccggacatg tgaatcagct cggccgcctg cttctgaatc gcggagacaa
     3481 ctttcggatg gcaatgtccg gtggagcaca cggcgatacc cgccgcgaaa tcgagaaagg
     3541 cgttaccgtc cacgtcttcc acaatcgcgc catgcccgcg cttggccacc agcggatagt
     3601 cgcgcgtata cgatggcgac atgaatttgg cgtcttgctc cagcacgcgc ttggcgttcg
     3661 gcccgggcag cgcagtcacg agccggggtg ttttcaattc caaagtcgtt ggcatgtctt
     3721 cctccatgga agaaatcaaa aggttggagt tgatcgagaa ctgcggggcg aaagcaggcg
     3781 cgcgaacgga cgaatccggg agtggccggc gagagaaaaa aaataagtta gttagtcgac
     3841 gccgtagagt tgtggccgct gggcggccgg cgggcagttc cccgggagga aagaagggcg
     3901 aatttgaagg ttaaggctca cttgcgtttc cagcggacgc tgccatcttt ggcgtcctca
     3961 atgattatac cacgatctgc gagttctttg cggatgcggt cggccgtggc gaaatcacgc
     4021 ttcttcttcg cggcgttgcg ctccgcgacc agccgatcga tctcctcatc gcccggtccg
     4081 ctctccgcgc tgccatatcc gagcgtgcga agcttgtcgg catcattgtc ctccacgacg
     4141 gcgaaaactt tgtcgaaagt agccagaaac tccagcaccg cggggacgtc accctggcgg
     4201 aattcgccct tatcgacggc gatattcgct tcgcgtacca ggtcgaacac tgccgccagc
     4261 gcccgcgccg tattcaggtc atccgcaagc ccggcgtcaa actcttccgc cgccttggcg
     4321 atgcgcgcgg ccataccctc ttgcctgccc gtgggaaatt ttccccgttt caaccggtcg
     4381 gcgaaattcc gcagccgttc caccgaactg gctgcctgct gcagaccgtc gaacgtgaaa
     4441 ttgagctgct tgcggtaagg aacacttgcg agagcaaacc gcagagccga aggcttgtaa
     4501 cctttcgcaa acaaatcacg cagcgtgaag aaattccctt gcgacttgga catcttttcg
     4561 ccttcgacaa gcaaatgctc ggcatgcagc cagtaccgcg cgaaaggctt gccggttgcg
     4621 ccctcgctct gcgcaatttc gttttcgtga tgcggaaatg ccaggtcgat tccgccggtg
     4681 tgaatatcca gcgtctcgcc gagatacttc atggccatgg ccgaacactc aatgtgccag
     4741 ccggggcgcc cgggcccgat cgaggtctcc cagaagtgct ctcccggttt cggcgctttc
     4801 cacaacgcaa aatcccgcgc gttctctttc tcgtagcgat cgttgtcgac gcgcgcgccc
     4861 acctggatgc cggtcagatc gatcttcgaa agctttccgt actccgggaa tttcgcgatg
     4921 cggtaataaa tcgaaccttc tcccggatac gtgaataatt tttcctggag ccgctggatc
     4981 agcttcacca tctcttcgat atggtccgtg gcccgaatcc agtgctccgg cgcctcgatg
     5041 ctaagcgtct tgcaatcttc gaaaaacgct tgtacgaatt tctcggtgta gtcgcggatg
     5101 cttttccctg ctgccgccgc attcgcgatg atgcggtcgt ccacgtctgt aaggttcatc
     5161 acgtggttca gcttgaatcc gcgaagtttc aggaaccgcc gcaaaatatc ctgaaacacg
     5221 aacgtgcggt aatttccgat gtgcgcgtag tcgtagaccg tcggcccgca cgtgtacatg
     5281 cgcacttcgc ccgctttttg tggaacgaaa ttctcgattt tcccgctcag cgtgttgtgc
     5341 agctgaagtt cggccatggg ctgtagatgg tctctttccg caaacgaata agcgttcgat
     5401 tgtatccgag cggtttgaaa cgtagcaatg tcgaagcagc gtgatgttgc gcggaactgg
     5461 gcgcaatacg acctgtttag tcctaattga cgctggccgc tcacgcggtt agagtcctcc
     5521 ttcccatcta gcgaaggagt tatcgatgaa tcgctctctt tcggctggcg cgctgtttgc
     5581 tctcgcggct ttcgccacgc ccattcttgc tcaggaaccg cccaaggcca gtgcgcccaa
     5641 actgctgact cccgaagctt ctttagacct gcgcagtatt tccgacctgc aattttcgcc
     5701 tgacggcaac cgtctggcgt ttgtggtcac ggagcccgcg aagggagagc accgcgcgcg
     5761 ccacatttgg atctacgaga agccgtccgg cgcgatccgg cagttcacct actctgcaaa
     5821 gtccgatttc ttgccgcgct ggtcacccga cggcaaacaa ctggctttcc tttcagaccg
     5881 cgacgaacag cagcaggtct acgctatgcg cgcaaacggc ggcgaagcca gcgcgctgac
     5941 caaaggaaag cgcggcgtga aggattttgc ctggtccccc gacggcaaac agattgcctt
     6001 tcttgccccc gatgcaaaaa ccgaggcgga agagaagaag gaaaaagaca aagacgacgc
     6061 ccacgtggcg gacaaagaag acaaacatgc ccgtctctgg ctcctcacgc tcgataccgg
     6121 agaagcccaa gccctcaccg aacccaaatg ggaaatcagc gaagctgcgt ggcatccttc
     6181 cggcacaggt ctgatgctca gcgccacgga tcaccccgag tcggatcaga acaccgagca
     6241 catttttttc tttcgtcttt gggacaccgc taacggcgac cacaaagcgg tcgatacgat
     6301 gacacaggtc cttgctccgc gcgggcactt cggcaatgtc cgcttggctg cggatggcct
     6361 gaggatggcg ttcgttggct gccgcgaaga cggccccacg ccgcacgacc tcatgctcgc
     6421 ccatcacggc gacaaggcca ctcagaacct cagcggcgcc agcgtggacc ggccggtctt
     6481 tgactaccgc tggtccaagg acggggggct gctcgctgtt gtggaggatg gcttccacac
     6541 gaagttcgtc gctttcactg cggaaggggc gctcaaggat gtcccggcga taccggcaaa
     6601 tcccaacgct ttcggcgttt ccgattccgg tgaggtcgtt tttgcagggc aaaccgcgac
     6661 gactccccag gaattgtggc tttgggatca gaagagtgca ccgaagcaaa tctctcactt
     6721 gaatgattcc tggaagcagt acacgcttag caccccggag ttctacaaat acaaatcctt
     6781 cgacggcatg gaaatcgagg cggctcttct gaaaccgctt ggccacgacg gcaaatcgaa
     6841 gctaccgctc gtcgccctga tccacggtgg ccccacgggc gcctggcagg actccatcga
     6901 aacctggggg cagctccttg cggcacgagg ctacgcggtc ttctatccca atattcgcgg
     6961 atcgagcggt tacggccaga aattcatcga gatgaatcgt ggcgactggg gtggcgggga
     7021 ttaccgggac gtcatggccg gtgtcgatga cctcgtcgcg cgcggcatcg ccgatcccaa
     7081 taagctggcc atcggcggct ggtcctacgg cggctacatg tcggagtggg cgataacgca
     7141 aaccacgcga ttcaaagctg ccgtctccgg cgctggcctc tcaaatctca tctccgaata
     7201 cggcaccgaa cagggcccct cctacgatga gtggttttac ggcgtcccct atgagccaga
     7261 aaagattgcc ggattcctga acagttcccc cttcgtccat ctcaaaaatg tgaaaacgcc
     7321 gacattgatt ctccaagggg acgccgaccc cgtcgatccc cccggccaaa gccaggaact
     7381 ctaccgcggc ctcaagcgct atggcgttga ggccgagctg gtcgtctacc ctcgcgagcc
     7441 tcacggcttt cacgaggaaa agcacttgct ggatcggctg aaccgtattc tcgcctggta
     7501 tgacaagcac ctgaaagaag gatcgccagc taccaggtag acccaagacg agacttcggg
     7561 cattccaagt acaagcttgt gccgctggca aagcggccac aagttgtagc gtagggcttt
     7621 acacacggtg cgcatcgtgt tactgttttt tcccgtaccg tcatccttgc gccaggcggc
     7681 tgggcctctg actgagaacc tgccaaacgt tttgacgggt gacccgagaa gggttgacct
     7741 gtcgctccaa agaccctccc ctcgaggaac gaaacgtcgt ctctcttcac gtgtttttgc
     7801 cccagccgta ggaaatggct ggctttcgca gttatggagt tgcattctag tttcacgctg
     7861 gcagccgcgg agaagttgac gaccaaaagg ggacatcttg ctgaagctgc gaaaagtgga
     7921 aatcgttggc tttaagtcgt ttttcgagcg caccgtggtc acgtttagcg gttccggcac
     7981 gacctgcatc gtcgggccga acggctgcgg caaatccaac gtcgtggacg ccatttcctg
     8041 ggttctcggc gaacagagcc acaaatcgct ccgcgccgag cgcatggccg attgcatttt
     8101 taatggcacc accaagcgcc ctccgctggg actcgccgaa gtcaccatca ccatggaaga
     8161 tgccgagctg gccgaagccg cccgcttcgt gatggaaagc gcggcggcag actcagcaac
     8221 ggattctgcg gcccttgtca ccgaaaacag cgaaacctct ggcttgaccg atgtgaccga
     8281 aattctctcg ctcgaccccg cgcaggcccc cgaaacgcag gaagctgccg ctgacggttc
     8341 agcaccatcc ccagctgaac cgggtggcaa gttcaaacgg aagaaaaaga cggcagacaa
     8401 gccggttctg gccatgaagc cgggcgaagt ggtcgtcagc cggcggctct atcgctccgg
     8461 ccaaagcgaa tacctgatca acggtc
//
LOCUS       DASYVO010000039         8750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_156267, whole genome shotgun sequence.
ACCESSION   DASYVO010000039 DASYVO010000000
VERSION     DASYVO010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8750
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156267"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VGR03_01375"
     CDS             complement(<1..368)
                     /locus_tag="VGR03_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992960.1"
                     /translation="MALTKRQKEVLDFLVAFETKHGYAPSFEEIGKGLKLTSLATVHK
                     HITTLEKKGFIRRGYNQSRSIEIVQLPKSVKDQVNDRKMQELPLVGRIAAGRPLEAVE
                     ERETISLGDFARGGNSFVLQV"
     gene            692..1510
                     /locus_tag="VGR03_01380"
     CDS             692..1510
                     /locus_tag="VGR03_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992961.1"
                     /translation="MICVSLFGIATGIGLIYLRHWARISILIWGGMSVFFGIFGTLIV
                     FLMPLSPTPNTADLPTGSMAAVRWIFLGIYGLPLLIGIWWLILFNRKTVKAQFVGARE
                     SADPGLPQKPSCPRPVAVLAWIYITAILNLLVLPFLPFRLPVFVFGRVLPGSVGWTVL
                     ILSCLASFVAGVGLLKLKPWSYSLTIGLQLFWLASTVVTVLTPKYNVVMDSMMKERQV
                     SLHLPESQFSSVNFALHYGWTVILGLVMAGAILGLLVYYRPRFIEAASRAASAS"
     gene            1799..3658
                     /locus_tag="VGR03_01385"
     CDS             1799..3658
                     /locus_tag="VGR03_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEV1992962.1"
                     /translation="MSTLAEVPPVKHRLEVLDQAVIRFAGDSGDGMQITGNQFTNTAA
                     LYGNDIATFPDYPAEIRAPAGTIPGVSGFQLHFSSNEVHTPGDAIDVLVAMNPAALKV
                     NVADLKANGILIVNSDSFQENDLRKAQMTSNPLEDHSLDKFRLFSVELERLTRRSLEH
                     LGLDAKSMDRCKNFFALGMCYWLYNRSSEPTVRWIEDKFKNKPLLVEANKLALKGGYS
                     YCEATEAFQVSYEIPPAQLTPGLYRSMSGNQALALGFVTAAQKSGLTLFQGSYPITPA
                     SDILHELSQYKDFGVVTFQAEDEIAAITSSIGAAYAGALAITTTSGPGMALKTEALGL
                     AVAVEIPLVICDIQRGGPSTGLPTKTEQADLLQALFGRNSEAPIPVLAASTPADCFWV
                     AIEASRIALKYMVPVIILSDGYLANGAEPWRIPELSDIPDFPVKFATDPNSFLPYKRD
                     PQTLARPWAVPGTPGLEHRIGGLEKQDPTGNINYEPLNHENMVRIRAAKVAAVAQDIP
                     NVLPAGDLEGDLLIVAWGSTSGSITAALKAQRARGRKIGHLHLRYLNPLPSNLGDVLK
                     RYKRVLVPELNMGQLLWVLRAKFLVDAVGLNKIQGRPFKQAELEQKIEEMLGL"
     gene            3670..4701
                     /locus_tag="VGR03_01390"
     CDS             3670..4701
                     /locus_tag="VGR03_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEV1992963.1"
                     /translation="MSTATLPPPLTKKDFQTDQEVRWCPGCGDYAILSAVQAVFPELG
                     IKKENFVIVSGIGCSSRFPYYMNTYGFHTIHGRAPAVATGIKAIRPELEVWVATGDGD
                     ALSIGGNHTIHMLRRNVGVKVLLFNNKIYGLTKGQYSPTSEFNKKTKSTPFGSLDRPF
                     NPMSLAIGAEATFVARSVDVFQGHLKETLKHAAAHKGSAFVEILQNCNIFNDGAWFSL
                     TDRDARSEHVMQLEHGKPLVFGKNRDKGIRRKSDGDIEVVQLGNGITESDLVVHDAHH
                     PRPSYAFLLSHMEHRPGFPTPLGVFRAWDDLPRYEDVMNQQIKDVIAKRGPGDLGKLL
                     RAGDTWEVK"
     gene            5038..5745
                     /locus_tag="VGR03_01395"
     CDS             5038..5745
                     /locus_tag="VGR03_01395"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEV1992964.1"
                     /translation="MLYERLHVTFVGVIATLVDSIVVAEFAGYWLHRLLHSDKFPALS
                     RGHLIHHFLIYGPRQPMRAGEYHDATYNRLSVGNVGIEWLVPSSIILLFCWGVMAVLG
                     VPHVYQVLVLCTLLGWPILMFSYVHDRMHIENFWMTRVPLLRSWFLKARRLHDIHHRS
                     VDSEGFMDTNFGIGFYFVDRFFRTMAKRHRPFNWEGYQAAIGRYGLEESELLSLRGCS
                     QALFHKEAGSKRDLRKT"
     gene            5934..7301
                     /locus_tag="VGR03_01400"
     CDS             5934..7301
                     /locus_tag="VGR03_01400"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEV1992965.1"
                     /translation="MDDLSNLGAFTLLGDADPVPQSLFVSYPEQAKTLMLLYEVSREL
                     TSILDLEELLRRVAERVKKIVNYDVFSVMLWNEQAQMLESVFAMRCTESIPARLRLPL
                     HQGLTGTAAGERRILRINDVTEDPRYIRCETGVEARSELVVPLLMQDRLIGVLDLEST
                     EPHAFTLEHERMLSTLGSYVAIALENARLFQEAKENERRMRSELDTAREIQRQLLPTG
                     AREVPGLDLAAAYVPARELGGDFYDFLPYGQGRLGFMLGDVSGKGTAAALYGSLAIGT
                     VREIAVDHTWEPADLLALLNQRLLGARLDSRFIAMLFAIYDAATRKLTLANAGGPYPL
                     LVRKGQVQAIRLEGVPLGLIPDTQYDETTIDVEPGDVVLFASDGILESENAAKEEFGP
                     VRLAALLSAISQGDSAREIAEQILAATDSHSGAGIAPHDDRTLLVLRVTEETASDFSK
                     LPIIY"
     gene            complement(7403..7879)
                     /locus_tag="VGR03_01405"
     CDS             complement(7403..7879)
                     /locus_tag="VGR03_01405"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEV1992966.1"
                     /translation="MKSRKNKCGFSLLELLIVVAIILIISAIAIPNLLRSKMAANEAS
                     AVGSVRAVNAACLNYSSTWGTGFPVSLSNLGPGKPATAAAADQVDSTVSGGTKGGYTL
                     TYVSGAPTNGKISTYTISASPVAPGKTGGRYFFADQSGVIRYNSGGAATVLSPPLS"
     gene            complement(8246..>8750)
                     /locus_tag="VGR03_01410"
     CDS             complement(8246..>8750)
                     /locus_tag="VGR03_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992967.1"
                     /translation="CGADQTRPVEIVDPNLPQPVTLKSTLHDWRTEIVVLVVVAGLIT
                     GIFLHNFREQSISPDLQAAGVAAKSLRDLREALSAYALSAKDVYPTTLDPLSARTSLP
                     TQSALSAGYKLQYIPNVTSNDGVPRGFVILARHEKSDYPSLYIDETGVVRATQENRPA
                     TVRDPPL"
BASE COUNT         1783 a         2560 c         2452 g         1955 t
ORIGIN      
        1 acctgcagga caaaggaatt gccgccgcgc gcaaaatcgc ccagcgaaat cgtctcgcgt
       61 tcttcaacgg cttcgagcgg acgccccgcc gcgatccttc ccaccagggg aagctcctgc
      121 atcttgcgat cgtttacttg atccttgacc gacttcggca attggacaat ctcgatggag
      181 cggctctgat tgtaaccgcg gcgaatgaag ccttttttct cgagcgtggt gatgtgctta
      241 tgcacggtgg ccagagaagt gagcttgagg cctttgccaa tttcttcgaa gctcggcgcg
      301 tagccgtgtt tggtctcaaa cgccacgagg aaatcgagga cttctttttg ccgcttggtc
      361 agagccatgt gcgcgcctcc ctttgcaaat cttttccaca ggcactttag gcgaaaagaa
      421 agcgaacgtc aagcttcttt tttccttcgc gttaccttca ccgctccctt ccgcgctacg
      481 tccggaagtg atatacaggc gttcgatgga tgactctccg aaacgctccg cctctgtgac
      541 tgtcgccgcg gtcgcggcca tcttgggcgg tcttttcttt cttctgtgtt tttcggccgc
      601 gattctcgta atgttgcttg tcaagttgcc cggcgctgcc tccgaactac caccattctt
      661 gagaaacttt acgctggtca cccagggatt catgatttgt gtgtcccttt ttggcatcgc
      721 caccggcatt gggctgatct atcttcgcca ttgggcgcgc atctctatct tgatttgggg
      781 aggcatgtcg gtttttttcg gcatctttgg gaccctaatt gtatttctga tgccgttatc
      841 tccaactccc aacaccgccg atttgcccac ggggagcatg gcagcggtcc gctggatttt
      901 cctcggcatc tacggcctgc cgcttctgat cggtatctgg tggctcatac tcttcaaccg
      961 caaaaccgtc aaagcacagt ttgttggcgc gcgagaatct gccgatccag gtctgccgca
     1021 gaaaccgtca tgtcctcggc ccgtcgccgt gctcgcttgg atttacatca ccgcgatttt
     1081 gaacctgctc gttcttccct ttcttccatt ccgcctgccg gtattcgtct tcgggcgggt
     1141 gctccccgga agcgtgggtt ggaccgttct gatactgagc tgcctggcct cttttgtcgc
     1201 cggcgttggc ctcttgaagc tgaagccgtg gagctactcc ctgaccatcg gcctccagct
     1261 cttttggctc gcgagtacgg tcgtgaccgt gcttacccca aaatacaacg tggtgatgga
     1321 ctcaatgatg aaggaaaggc aggtgtcgct gcatctacct gaatcacaat tctcatctgt
     1381 aaacttcgcc cttcactacg gctggaccgt aattctcggg ctcgtaatgg ccggcgcaat
     1441 tctcggtttg ctcgtctact atcgtccacg cttcatcgaa gcggcttcca gagctgcctc
     1501 tgcatcctga cgacgcgccc tccctagaat gctttctgcg ccgcggaatc tgcctccact
     1561 cttgcgcggc gagcagaact ggcaatcgat ttgaagcgtc tttcccgata ggacacatcg
     1621 cgtcgcaggg gactctccat ctgccacgcg cgggccggtg tgatatcctt tgagtgtcgg
     1681 ctcccggcgg gctgccttag aaaccatcct tttaccgcac cgcctcatcc ttatgattga
     1741 gccacactcc cctggcggac aaagggagtt catcgatgtg cggaggttgg ggacaagaat
     1801 gtctacactc gcagaagttc caccagtaaa acacaggctc gaagtgctcg atcaggcggt
     1861 catccgattc gcgggcgact ccggcgacgg catgcagatc accggcaacc agttcacgaa
     1921 taccgccgcg ctctacggca atgatattgc tacgtttccg gactatccgg cggaaattcg
     1981 cgcccctgca gggacgattc ccggtgtcag cggctttcag ctgcacttct cttcgaatga
     2041 ggttcacacg cccggcgacg cgatcgacgt gctggtcgcc atgaatccgg ccgcgctaaa
     2101 agtgaacgtt gccgacctga aggccaacgg gatcctgatc gtcaacagcg atagtttcca
     2161 agagaacgat ctgcgcaaag cgcagatgac ctcgaatccg ctggaagatc attccctgga
     2221 taagttccgg ttgttttcgg tcgaactcga acggctgacg cggcgttcgc tggagcatct
     2281 gggcctcgac gccaagtcca tggaccgctg caaaaacttc ttcgcgctgg gcatgtgcta
     2341 ctggctctac aaccgctcga gcgagccgac ggtccggtgg atcgaggaca agttcaagaa
     2401 caagccgctg ctcgtggaag cgaacaagct cgcgctgaaa ggcggctatt cgtactgcga
     2461 agcgacggaa gcgtttcagg tcagttatga gatcccgccg gcgcagctta ccccgggact
     2521 ctacagaagc atgagcggga accaggcgct ggcactgggg tttgttaccg cggcgcagaa
     2581 atccgggctg acgctctttc aaggcagtta ccccattact cccgcatcgg acattttgca
     2641 cgagctgtcg caatacaagg acttcggcgt ggtgacgttt caagccgaag acgaaatcgc
     2701 ggcgattact tcgtcgattg gcgcggcgta cgccggcgcc ctggccatca ccacgacgtc
     2761 cggcccggga atggccctga aaacagaagc gctgggcctt gccgttgcgg tcgagatccc
     2821 gctggtcatt tgcgacatcc agcgcggcgg tccgtcaact ggccttccga ctaagaccga
     2881 gcaagccgac ctattgcagg cgctttttgg ccgcaactcc gaagcgccga ttccggtgct
     2941 ggccgcatcg acgcctgcgg attgcttctg ggtggcgatc gaggcgagcc ggatcgcgct
     3001 caaatacatg gtgcctgtca tcattctttc agacggctac cttgcaaacg gcgcggagcc
     3061 gtggagaatt ccggagttga gtgatattcc ggattttccc gtgaaattcg caaccgaccc
     3121 gaacagcttc ctgccttaca agcgcgatcc gcaaactctg gcgcgcccgt gggcggttcc
     3181 gggaactccc gggctcgaac atcgcatcgg tggcctcgag aaacaagacc ccacggggaa
     3241 cattaactac gagccgctga atcacgaaaa tatggtgcgc atccgggcgg cgaaggttgc
     3301 agcggtcgct caggacattc ccaatgtgct tcccgcaggc gatctggaag gcgacttgct
     3361 catcgttgca tggggctcga cgtctgggtc gattacagcg gcgctgaaag cgcagcgtgc
     3421 aaggggccgc aagatcggac acctgcacct gcggtacctc aatccactcc cgtcgaattt
     3481 gggtgacgtt ctcaagcgct acaaaagagt gcttgtgccg gaactgaaca tgggccagct
     3541 tttgtgggtt ctgcgcgcaa agtttctcgt ggatgccgtc ggccttaaca agattcaggg
     3601 ccggcctttc aagcaagctg agctggaaca aaaaatcgaa gagatgttgg gactctagga
     3661 gaatcgacga tgagcacagc aacgctgcca ccgcctctca caaaaaaaga ctttcaaacg
     3721 gaccaggaag tgcgttggtg tcccggctgc ggagattacg cgattctctc ggcggtgcaa
     3781 gccgttttcc cggagctcgg gatcaaaaag gagaatttcg tcatcgtctc cggcatcggc
     3841 tgttccagcc gctttccgta ctacatgaac acgtacggct tccacacgat tcacggccgc
     3901 gcgccggcag tggccacggg catcaaggcg attcgcccgg agttggaagt ctgggtggcc
     3961 accggcgacg gcgacgcgct ttcgatcggt gggaatcaca cgattcatat gctgcggcgc
     4021 aacgtcgggg tcaaagtcct gctcttcaac aataaaattt atggtttgac gaaaggccag
     4081 tactcgccga cctcggagtt caacaagaag acgaaatcaa cgccgttcgg gtcgctggac
     4141 cggccattca atccgatgtc actggccatc ggtgcggaag caaccttcgt ggcacgctcc
     4201 gtcgacgtct tccaggggca cctgaaagag acgctgaagc acgccgcggc acacaagggc
     4261 tccgcgttcg tggagattct tcagaactgc aatatcttca acgatggggc gtggttctca
     4321 ctgaccgacc gcgatgcacg cagcgagcac gtgatgcagc tcgagcacgg caagccgctg
     4381 gttttcggca aaaaccgcga taagggtatc cgccggaaat cggacggcga cattgaagtc
     4441 gtccagctcg gcaacggcat cacggagagc gatctcgtgg tgcacgacgc gcaccacccg
     4501 cggccctcct acgccttcct gctctcgcac atggagcacc ggccgggctt ccccacgccg
     4561 ctcggcgttt ttcgcgcgtg ggacgatctc cctcgctacg aagacgtgat gaaccagcag
     4621 atcaaggacg ttatcgccaa gcgcggtccc ggcgacctcg gaaagctcct tcgcgccggc
     4681 gacacctggg aagtgaaata gttttctgtc tgactaccgc atagattctt caagaagaaa
     4741 gaaggccgcc gcagccgtca aggccatggc gccctctctc ttagaagcgg gatctgccca
     4801 caacctcctc tgattcgtac ttcgcggtaa gcgagacgac acatctttct ccctgtaaat
     4861 gctttccttt caattcgcca agcgcaatat cccgcgacat tgtgcgacat cccgcgctcc
     4921 cggcgccttg gcgttccttg tcgcttgagc tctgcttgcg cactgggtag ggtcatcaca
     4981 ccagcggagt cgtttttcgg ttcggaaatg gatagcgaag aacggtagga ggagtccatg
     5041 ctttacgaac gcctgcacgt cacattcgtc ggagtcatcg cgaccctagt agactccatc
     5101 gttgtcgcgg aatttgccgg ctattggctg caccggcttc tgcacagcga caagttccca
     5161 gccctcagcc ggggccatct gatccatcat ttcctgatct acggcccccg gcaacccatg
     5221 cgcgccgggg agtaccacga cgcgacgtac aaccgcctct ccgtcggcaa tgtggggatc
     5281 gaatggctgg tgccctcctc gatcattctc ctgttttgct ggggagtgat ggcggtgctc
     5341 ggcgttccgc acgtgtacca agtgctcgtg ctgtgcacgt tgctcggctg gcccatcctg
     5401 atgttcagct acgtgcacga caggatgcac atcgagaact tctggatgac gcgagttcca
     5461 ctgcttagat cctggttcct caaagccaga agactgcacg acattcatca ccgcagcgtg
     5521 gacagcgaag gtttcatgga cactaacttc ggcatcggct tctactttgt tgaccgattc
     5581 ttccggacca tggccaagcg gcatcgcccg ttcaactggg agggctacca ggcagccatc
     5641 gggcgctacg gattggagga atcggagctc ttgtcattgc gcggctgcag tcaggcgctg
     5701 tttcacaaag aggctgggag taaaagggac ttgcgcaaga cttgaattgc cggcaacttg
     5761 cgaagcgttg attcttcagc tagatgaata ccctcagtca aggtgcgctt cccctggact
     5821 tttcttccgg cccatgtctt tcccggggtt tttgtccatt gtttgaaaat tgaattgtga
     5881 aaccttctgg gatttgctac atccaataga ggctaggcgc tgaacaccct gccatggatg
     5941 acctttcaaa tctcggagcc ttcacccttc taggtgacgc ggaccccgtg ccgcagagtc
     6001 tgttcgtcag ctatccggaa caggctaaga cattaatgct gctgtatgag gtgagccgcg
     6061 agctgacctc gattcttgat ctcgaagagc ttctccgccg tgtcgccgaa cgcgtcaaga
     6121 aaattgtgaa ttatgacgtc ttcagcgtca tgctctggaa cgagcaagcc cagatgctcg
     6181 agagcgtttt cgccatgcgc tgcacggaat cgattcccgc gcgcctccgc ctccctcttc
     6241 atcagggcct cacaggaact gccgccggag aacgccgcat cttgcggatt aatgacgtca
     6301 ccgaggatcc tcgttacatc cgctgtgaaa ccggcgtgga agcgcgttcc gagctcgtcg
     6361 tgccgcttct catgcaggac cgcctcattg gggttctcga tctggaaagc acggagccgc
     6421 atgcgttcac cctggaacac gagcgcatgc tgtctacgct tggctcctat gtagccatcg
     6481 cactcgagaa tgcccggctc ttccaggaag ccaaggaaaa cgagcgccgg atgcgcagcg
     6541 aactggacac cgcaagagag attcagcggc aactccttcc caccggcgcg cgcgaagtgc
     6601 cgggccttga tttggcagcc gcctatgttc ccgctcgcga gttgggcgga gacttttacg
     6661 actttctgcc ctatggacag ggccgcctgg gattcatgct cggcgatgtt tccggcaaag
     6721 ggaccgcggc ggcgctgtac ggttcactgg cgatcggcac cgttcgagaa atcgccgtcg
     6781 atcacacctg ggagccggct gacctgctcg ctcttttgaa tcagcggctg cttggcgcgc
     6841 ggcttgattc gcgattcatc gccatgctgt tcgccattta cgacgccgcg actcggaagc
     6901 tcacgctcgc caacgctggc ggaccgtatc ccctcctggt ccgcaaaggc caggtacagg
     6961 cgatccgcct ggaaggcgtg ccgctcggtc tcattcccga tacccaatat gatgagacga
     7021 ccatcgacgt ggagccggga gacgttgttc ttttcgcttc cgatggcatc ctggaatcgg
     7081 agaatgccgc gaaggaagag tttggtccgg tacggttggc ggctcttctg tccgctattt
     7141 ctcagggaga ttcggcgcgc gagatcgccg aacaaatcct ggctgcaaca gacagccaca
     7201 gcggcgcagg cattgctccg cacgacgatc gcaccctgct cgtgctccgg gtaacggaag
     7261 agaccgcctc cgatttttca aaacttccca tcatctactg aaaatctcac accgtatcag
     7321 atgatcttcc cggttattct ctgcggtagc cggtgttggc tcatttctgg attcgcaccc
     7381 tactaaagca ctaacgtctg ttctaactga gcggaggact caagactgta gcagcgccgc
     7441 cggagttgta acgaatcacg ccagattgat ctgcgaagaa gtaccgtccc cccgttttgc
     7501 cgggagccac cgggctggca ctgatcgtgt acgtcgatat tttgccgttg gtgggagcgc
     7561 cggaaacata agtgagcgta tagccgccct tggtcccacc ggacaccgta ctatcaacct
     7621 gatcagcagc agcggcggtt gcgggcttcc ctggccccag attggacaag ctcacgggaa
     7681 agccggttcc ccaagtcgaa gaatagttga ggcaagcggc attcacggcg cgaacggagc
     7741 ctaccgccga agcttcgttc gcggccatct tagagcgaag aagattggga attgcaatgg
     7801 ccgaaatgat caaaataatg gccacaacga tgagcagttc cagaagggaa aatccacatt
     7861 tgttttttct gcttttcatt tcgatttaga ttccttggcc tgctgcattg ctgtcgattt
     7921 gaatggcacc tatcggtcca cacttcaggg cctggatgaa cagttgtaac caagagctat
     7981 ctaacgcaca cactggaact taagccttga gaaatcattc cggcagcgtg gaaatggtct
     8041 gggcttcggt catcacggag gttcaatttg gaatgaatgt tccaatctgg gccctctttc
     8101 gaatcaaagc ggtttgtgtt tgcagggagc gaagggcaga tctaactgag aatggaatat
     8161 tgcgctcagt tcatggaaag cagtgggcga tgcggtaatt cgcatatcgc attctgtagg
     8221 cagcggaaga ctattctgaa gagtctcaga ggggtggatc ccgcaccgtg gctggccggt
     8281 tttcctgcgt tgctcgcacc acgccggtct catcgatgta gagacttgga tagtcgcttt
     8341 tttcgtggcg cgccagaatc acaaagccgc ggggaacacc gtcattcgag gtgacattcg
     8401 gaatgtactg caacttgtag cccgcgctca gcgcggattg cgtgggtaga ctggtccgag
     8461 cactcaacgg atccaaagtc gtgggataaa cgtctttcgc agaaagagca taggccgata
     8521 gcgcttcgcg aaggtctcgg agcgacttgg ctgcgacccc cgcagcctgc aagtcaggac
     8581 tgatactctg ttccctgaag ttgtgcaaaa aaatgccggt tatcaggcca gcgacaacga
     8641 ccagcaccac gatttcggtg cgccagtcgt gaagagtgga cttcaaggta acaggctgcg
     8701 gcaaattggg atcgacgatt tcgacggggc gggtttggtc tgcgccgcat
//
LOCUS       DASYVO010000040        10743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_57039, whole genome shotgun sequence.
ACCESSION   DASYVO010000040 DASYVO010000000
VERSION     DASYVO010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 10743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10743
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57039"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VGR03_01415"
     CDS             <1..623
                     /locus_tag="VGR03_01415"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1992968.1"
                     /translation="LMAGWTEGIAFALGASIATTQEWLGKMGGFAAIADVLADDYELG
                     HRIAKAGGEVLLSREAAWTMYPAQTLRSFWDHQVRWARTVRLCRPLSYVGLLFTQGLP
                     WVLAAALVAPARWIGGAYLLAYLILRFAMAWTVGIWGVRDEVLRRKIWLLPLRDAIHF
                     AIWLASFGSNHIRWGNVEYVIRKGRMVPIAGSEGAAAKPAEDAPRS"
     gene            752..825
                     /locus_tag="VGR03_01420"
     tRNA            752..825
                     /locus_tag="VGR03_01420"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:784..786,aa:Gln,seq:ctg)
     gene            complement(794..2140)
                     /locus_tag="VGR03_01425"
     CDS             complement(794..2140)
                     /locus_tag="VGR03_01425"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1992969.1"
                     /translation="MTSERVFYGWKLVFILWLLDFLNMGFPLYGGAVINTYMLKEIPM
                     SRSMFGLGFTLLNLFVGIPSILVAASIVRWGIRKTFGIGSALILVGALWLSLIASRPW
                     HYWVGFGVLTATGISFGTIVPAATAVTRWFSRYRGRAMAVTLSASGFAGFFVSPLINR
                     ILTTNGGNWRQAWAVVAGISVLSAVVAFLFVKERPEDLGQSVDGGPGGAQPAAGSPAT
                     NKLVTTFPWEPRQAYRTLSYWMILIGGVACQFPFFFFTAHWLLHLKGVGISPADAAWA
                     MGLFTLGAVFGRLIGGWLMDRMVARYAFMLGLCLYFLGSFLAIRVSPDALWVAYTAAI
                     LYGTAFGWTFICLNTVTGHFYGPAAFPRLNGMLLVIGAVFCSPAGFLGGKLFDTYHSY
                     KLAFELNSLVTAVGIVALFFARMPEPPEAETQVSERVRSRTERAKKLAGRPGFEPG"
     gene            2665..3456
                     /locus_tag="VGR03_01430"
     CDS             2665..3456
                     /locus_tag="VGR03_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEV1992970.1"
                     /translation="MQKALRLLLHLGEYGPEMGITQLAGELRLNKTTVHRLLNAMQKF
                     ELIEKNPANERYRLGLKLHELGCRALESRTLRGEAHEFLVEMSRRCNESVSLAVPGAG
                     SIVCLDRVDCLDSVITARTPVGGRFHPHCTAAGKAILAYLPDEEIRAIIKRNGMPRFT
                     SATISKYADLMNDLALTRQQGYASDAGELEKGLSGIAAPVFMRGSDLVAALGIAGPSG
                     RFQLEELADKIALVKDFAARLSRAMRRRASELPAASRGSFYDPLS"
     gene            3470..4591
                     /locus_tag="VGR03_01435"
     CDS             3470..4591
                     /locus_tag="VGR03_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992971.1"
                     /translation="MAAPYRADHIGSFLRPAELLAARSNAAKDPERLRTLEDLHIQRI
                     LAKQKELGFELATDGELRRRNFMSDFTDAVDGFDMSDAVGRNWKAGEMKAAPVSSVTG
                     IVGKKLRQTRPLTGHELPFLKAKCTVAIKMTLPSATQFPAISFKDGITDKIYKDHSAL
                     LWDIVEIMKSDLAKLSSEGVNYIQIDAPRYSYYMDPKWREWIRTELKTDPDAALDEAL
                     KADNACFKAARRPGVTLAIHLCRGNNRSHWYAEGGYDAIAEKMFSTLDVDRFLLEYDD
                     ERSGTFAPLRFIPKGKTVVLGLVSSKLPQMEDPNVLVKRIEEASQYVPMENLALSPQC
                     GFASTAEGNLLTEDRQWAKLKLVVDTAHRVWGGASSAGA"
     gene            4726..6165
                     /locus_tag="VGR03_01440"
     CDS             4726..6165
                     /locus_tag="VGR03_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV1992972.1"
                     /translation="MFSTPYIAGKAIDVPPSALSDVVNPANQRPFARIFMAKAEHMRA
                     AIDAADAAKASWAAAPPAVRERILHRAADELEKATPELVELLIDEAGSTFGKAHFEVG
                     FAANMVRSIAGEARRIHGDVMASDVPGLISLAIRRPLGVVAGISPFNFPIVLSLKKVA
                     FALAAGNTFILKPSEETSLVGLKLAEVFERAGVPAGVLNVVPGDGPTMAQALYEDPRV
                     KLISFTGSTRVGRIIATECAKRGKRVVLEMGGKSPLIVLRDADLYYAVDTACFGLFIH
                     QGQICMAGSRIIVEAPVYEEFLKRFAAKAQTLQVGDPRDPHTVIGPLIRSSQCPMIDK
                     KVKDAVAAGARLLCGGKYEGNFYHPTVLADVTPEMAAFRDELFGPVAAVSKANDADHA
                     LALANHSAYGLSSAVLTNNMQLAMKFALELEAGMVHLNGPTVHDEATVPFGGVKDSGM
                     GREGGRWSIEEMTEVKWVTIQMGKRMYPF"
     gene            complement(6342..6908)
                     /gene="pcaG"
                     /locus_tag="VGR03_01445"
     CDS             complement(6342..6908)
                     /gene="pcaG"
                     /locus_tag="VGR03_01445"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEV1992973.1"
                     /translation="MSLQATTSQTVGPYFSIGLTRLRKVDLVGAGVSGERVTIAGRVV
                     DGDGNPVSDALLEIWQANSHGKYAHPEDNQNKPLEPGFQGYGRMGVDENGRFAFTTVK
                     PGAVPSPDGKMQAPHIAISVFARGLLLRLVTRIYFPEESANADDPILNLIEHARRGTL
                     IARKTAVKGALEWDVILQGAGETVFFDC"
     gene            complement(6910..7614)
                     /gene="pcaH"
                     /locus_tag="VGR03_01450"
     CDS             complement(6910..7614)
                     /gene="pcaH"
                     /locus_tag="VGR03_01450"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEV1992974.1"
                     /translation="MTNIAGYRRPDPGTQPKHLHPPYISSVKRAPSRPLVYMPHTLTE
                     VTGPLFGKEAIDLKACDLTRQHAGEPLGERIIVSGRVVDEDARPVPHTLVEVWQANAA
                     GRYLHEADQHDAPLDPNFTGCGHTLTDGEGRYRFVTIRPGAYPWRNHHNAWRPAHIHF
                     SIFGPAFATRLVTQMYFPGDALIPYDPIFNCTADEQARNRLISVFDWGSTIPDQALGY
                     RFDVILSGRETTPMEK"
     gene            7899..8768
                     /locus_tag="VGR03_01455"
     CDS             7899..8768
                     /locus_tag="VGR03_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEV1992975.1"
                     /translation="MPVADSNPSVRKQSRTRSVSAALLESVQSPLPPFEGAGGDPNFM
                     TSLARGLAVIQAFSQTKRQQTVSQISSKTGFSRAAVRRCLYTLAKLGFAGSDDNRHFH
                     LRPRVLTLGHSYISSMPLAVAAQPVLEHVSHVLHESCSIATLDGPEIVYIARATVTRI
                     MSIDLGVGSRLPAFCTSMGRILLADLPPEKLEELLASIEFTRYTERTVTSVEKLRQIL
                     RIVQRNGYSVIDQELEYGLRSMAVPIRNPTGKVVAALNVGAHAQRVSLQDLLMRFLPH
                     LKAAAQELCMFLK"
     gene            8863..10101
                     /locus_tag="VGR03_01460"
     CDS             8863..10101
                     /locus_tag="VGR03_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1992976.1"
                     /translation="MDNSKRYENTLVAILFFSWGTVFLDRTSQHYLAPYFAPEFHLSH
                     QQIGMLGSVVAVTWALSTLFFGALSDRVGRKPILVPVVFIFSMLSWISGMVHSFHQLL
                     LVRALLGLAEGPAWSIMTAMIEESSHPTRRGRNIGIVVSAAALVGLAVAPVLTTQVAA
                     RMGWRWAFFVSGVPGFLLGLLIWKFVKEPERGNAAGAHGQAVKIRDFLSVLRYRNMWL
                     CCAGSAAFLSWLFLHNTFGPLYITEVAHQAPTTAGFLMGASGLGSFFLALYFPRLSDR
                     IGRKPVLLVMAGMCMIVPLALLISPLYAYPWVLAAIVCLANAGQGIGALILVLVPTES
                     VPPQFAATAIGLATLAAEIIGATLAPILAGRVAQSYGLGATMWMSAGGAVLVFVVALF
                     LKETHLARAGRPALQEAPAE"
     gene            complement(10237..10335)
                     /locus_tag="VGR03_01465"
                     /pseudo
     CDS             complement(10237..10335)
                     /locus_tag="VGR03_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(10317..10475)
                     /locus_tag="VGR03_01470"
     CDS             complement(10317..10475)
                     /locus_tag="VGR03_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV1992977.1"
                     /translation="MDPFNCPVTVTTHIIGGKWKPSILYYLQGRIRRFSELHKLLSGP
                     TKKCLRSN"
BASE COUNT         2274 a         3041 c         3231 g         2197 t
ORIGIN      
        1 tgctgatggc gggatggacg gaaggaatcg ccttcgcgct gggggcttcc atcgccacga
       61 cccaggaatg gctcgggaaa atgggcgggt ttgcggccat tgccgatgtg cttgcggacg
      121 actatgaact cggccaccgc atcgccaaag cgggcggcga agttctcctc tcacgcgaag
      181 ccgcctggac catgtatccc gcgcagacgc ttcgcagctt ctgggaccat caagtgcgct
      241 gggcgcgtac agtgcggttg tgcaggccgc tgtcctatgt ggggctgctt tttacccaag
      301 ggcttccctg ggttctggct gccgcactcg tcgcgccagc gaggtggatc gggggcgcct
      361 atctgcttgc ttacttgatc ctgcgttttg cgatggcctg gacggtgggg atttggggcg
      421 tccgggacga agtcctgcgc cggaagattt ggctgcttcc gctgcgggac gcaatccact
      481 tcgcgatctg gctggcgagt tttggttcga atcacatccg ctggggcaac gtcgaatatg
      541 tgattcgcaa gggccgcatg gtgcccatcg ccggcagcga gggcgcggcg gcaaagcccg
      601 ccgaagacgc cccgcgatcg taaatttgtg ctgcgctatt cgcggcgtac ggttgacggt
      661 tacaggaaac ccgattaaat tagataggta gaatcgtagc tgccccgatc atcgggaacg
      721 cgctgcgatt cgtttttttc ctcgccacgg gtgggagatc gtccaacggt aggactccag
      781 attctggatc tggctatcct ggttcgaatc caggtctccc agccaacttt tttgccctct
      841 ctgttctact tctcacacgc tcactcactt gagtttccgc ttccggcggc tccggcattc
      901 tcgcaaaaaa gagggcaact atgccgacgg ctgtgaccag ggagttgagt tcgaaggcca
      961 gtttgtagct gtgataggta tcaaacagtt ttccgcccag aaagccggct ggggagcaaa
     1021 aaacagctcc gattaccagc agcataccgt tcaatcgtgg gaatgctgcc ggtccataga
     1081 aatgccccgt gacggtgttc agacaaataa aggtccagcc gaaagcagtg ccatagagaa
     1141 ttgccgcggt gtaggcgacc cagagcgcgt ccggagaaac ccggatcgcc aggaatgagc
     1201 cgagaaaata gaggcacaaa ccgagcatga acgcatagcg cgcgaccatc cggtccatga
     1261 gccatccgcc aatcagccgg ccgaaaacgg cacccagggt gaacagaccc atcgcccaag
     1321 cggcgtccgc gggagaaatc cccaccccct tcaagtgcag cagccagtgg gcggtgaaaa
     1381 agaaaaacgg aaattggcaa gcaacgccgc cgatgagaat catccagtag gagagtgttc
     1441 ggtaagcctg tcgaggctcc cagggaaacg tggtgaccag cttatttgtg gccggtgacc
     1501 ccgccgccgg ctgtgcaccg cccggtccgc catccacgga ctggccaagg tcttccgggc
     1561 gctctttgac gaacagaaaa gcaaccaccg cggatagaac ggaaatgcca gcgaccactg
     1621 cccacgcctg ccgccagttc cccccattcg ttgtgagtat ccgattgatc agcggcgaaa
     1681 cgaaaaagcc tgcgaagccc gacgcggaga gtgtgacagc catcgctctt ccgcgatagc
     1741 ggctgaacca gcgcgtgacg gccgtggctg cgggaacgat ggtgccgaaa ctgattcccg
     1801 tggctgtgag cactccgaac cccacccaat agtgccaagg ccgggaagca atcagggaga
     1861 gccatagggc cccaacaaga atcagcgcgg agccgatgcc aaatgttttg cgaattcccc
     1921 agcgaacgat ggacgctgca acaaggatcg agggaatacc cacgaagagg tttaagagcg
     1981 taaatcccag cccgaacatg ctgcggctca tgggaatttc tttcaacatg taggtgttga
     2041 tgaccgcgcc gccgtacaag ggaaatccca tattgaggaa atccagaagc caaagaatga
     2101 aaaccagctt ccagccgtaa aagactcgtt ccgaggtcat atcgagtttt gtccgtggcg
     2161 cggtccgtgc gcgcagccct ggtgttacgc ccaacaacaa agagcttgga gaatatcaga
     2221 ttctcgagga atcggaaaaa accgcaattt tgtccgcagg agaaatattg cctttgccat
     2281 gccaatgccc ttgccattgt catccggagc gaagcgaagg atctcatata aatccacact
     2341 tcgtcgtaaa cattcacttg aaacccttcg ggctagcccg ccgcgccgcg tgcaaagggc
     2401 gcgtggttga tctcgtggcc ccaggcgacg gcgtaccaca ggtcacgcag gaacacggcc
     2461 gaattccttt cgaatcgcct gtttcataat gtgaaactga gtttcatatt gacttcgaag
     2521 tgattctatc tacaatgcac ccatcgtcaa gcgaactctc cttcgctccg agacgcatgg
     2581 aagggaacag atttgccagt tgcactgcgg cgaagcaaaa gactcagctc ccggcgaagc
     2641 gatggcgcgg ggtcgagcaa atccttgcag aaggcgcttc ggctactgct gcatctcggg
     2701 gaatacggtc ccgagatggg catcacgcag cttgccggcg aactccgttt gaataagacc
     2761 accgtccacc gtctcctgaa cgccatgcag aaattcgagc ttatcgaaaa aaatccggcg
     2821 aacgaaaggt accggcttgg tttgaagctg cacgagttgg gatgccgcgc cctggaatcc
     2881 aggacgcttc gcggtgaagc gcacgaattc ctggtggaaa tgtcccgtcg ctgcaatgaa
     2941 agtgtgagcc tcgcggtgcc gggagcggga agcatcgtct gcctcgatcg ggtcgattgc
     3001 ctggactctg tcatcaccgc gcggacaccc gtcggcggtc gttttcaccc gcactgtacc
     3061 gccgcgggca aagcgattct ggcctacctc cccgatgagg aaatccgcgc catcatcaag
     3121 cgcaacggca tgccccgctt cacctccgca accataagca aatatgcgga cctgatgaac
     3181 gatctcgctt tgacgcgcca gcaaggttac gcgagtgacg ctggggagtt ggaaaaagga
     3241 ctcagcggca ttgccgcgcc ggttttcatg cgcggcagcg atctcgttgc agccctcggg
     3301 atcgctggcc ccagcgggag atttcagctt gaagaacttg cggacaagat cgctttagtt
     3361 aaagatttcg ctgctcgcct ttccagagcg atgagaagac gcgcgtcgga gttgccggcc
     3421 gcgagtcgcg gttcgttcta cgatccgctc tcataggagg tatacagaaa tggcagcacc
     3481 ataccgagcc gaccatatag gaagttttct ccggcccgcg gaactcttgg cagcgcgaag
     3541 caacgccgca aaagacccgg agcgcctgcg caccctcgaa gatcttcata tccagcgcat
     3601 tcttgccaag cagaaagagc tcggttttga actcgccacg gatggcgaac tgcgccgccg
     3661 gaattttatg agcgatttca ccgatgcggt ggatggcttc gacatgagcg atgcagtagg
     3721 gagaaactgg aaagccggag aaatgaaagc ggctccggtc agcagcgtga cgggcattgt
     3781 gggcaagaag ctgcgccaaa cgcggccgct caccgggcac gaattgcctt tcttgaaagc
     3841 gaaatgcacg gtggcgatca agatgacgct tcccagcgcg acgcaatttc cggccatttc
     3901 gttcaaggac ggtatcaccg acaagatcta caaggatcac tccgcgttgt tgtgggacat
     3961 cgtggagatc atgaagagcg acctcgccaa actgtcatcc gagggcgtga actatatcca
     4021 gattgacgcg ccgcgctaca gctattacat ggaccccaag tggcgcgagt ggattcgtac
     4081 cgagttgaag accgatccgg acgcggcgct cgatgaggcc ctcaaagccg acaacgcttg
     4141 cttcaaggcc gcgagacgcc caggcgtgac gctggcgatc catctttgcc gcggcaacaa
     4201 tcgcagccac tggtacgccg aagggggcta cgacgccatc gccgaaaaaa tgttcagcac
     4261 tctcgatgtg gaccgcttcc ttctggagta tgacgacgaa cgctccggaa cgtttgcgcc
     4321 gctgcgcttc atcccgaaag gcaagaccgt ggtgctgggg ctggtcagtt ccaaacttcc
     4381 gcaaatggaa gatcccaacg tgcttgtgaa gcggatcgaa gaagcgtcgc agtacgttcc
     4441 catggaaaat ttggcgctga gtccgcagtg cgggttcgct tccacggcgg aaggaaacct
     4501 gctcaccgag gaccggcagt gggccaagct gaaactcgtg gtggacacgg cgcatcgcgt
     4561 gtggggcgga gcgagcagcg cgggcgcttg acattccaca agcagtggcg aaaaatcccc
     4621 attcggacac acgcgcgagt gtgcgattag cgttcgaatc gttttgtgta aattgtactt
     4681 gctgacccga ttctctttcc gcaaagtgca tcacgtgagg aaaccatgtt ctcaacacct
     4741 tatatagccg ggaaagcgat tgatgtcccg ccatccgctc tctcggatgt cgtcaacccc
     4801 gcgaatcagc gtccgtttgc ccgcatcttc atggccaagg cggagcacat gcgcgcggcg
     4861 attgacgccg cggacgccgc gaaagcgtcg tgggcggcag cgccgccggc cgtgcgcgag
     4921 cgaattctgc accgagccgc ggacgaatta gagaaggcca cgccggagct tgtggagttg
     4981 ctgatcgacg aagccggctc gacatttggc aaagcgcatt ttgaagttgg gtttgcggcg
     5041 aacatggtgc gctcgattgc gggagaggcc aggcgcatcc acggcgacgt gatggcttcc
     5101 gacgtgcccg ggctgatctc gctggcgatt cgccggccgc tcggggtagt ggccggcatc
     5161 tcaccgttca attttcccat cgtgctctct ctgaagaagg tggcgttcgc gctcgcggcc
     5221 ggaaacactt tcatactcaa gccgtccgaa gaaacatcgc tggtggggtt gaagcttgcg
     5281 gaagtctttg agcgggcggg ggtgcccgcg ggagtattga acgtggtgcc gggcgacggg
     5341 ccgaccatgg cgcaagcgct gtacgaagat ccccgcgtga agctgatttc ttttaccggc
     5401 tcgaccagag tcggaagaat catcgcaacg gagtgcgcca agcgcggcaa gagagtcgta
     5461 ctggagatgg gcggcaagag cccgctgatc gttttgcgcg acgcggatct gtactatgcc
     5521 gtggacacgg cgtgcttcgg attgttcatt caccagggac agatctgcat ggccggctcg
     5581 cggatcatcg tggaagcacc ggtctacgag gaatttctga aacgatttgc ggccaaggcg
     5641 caaacgctgc aggtcggcga tccgcgcgat ccgcacacgg tgattggacc gctgatccgc
     5701 tcatcgcaat gcccgatgat cgacaagaag gtgaaggatg cggtggccgc gggcgcacgg
     5761 ctgctctgcg gcggaaaata cgaaggaaat ttctatcacc cgacggtcct ggcggatgta
     5821 acgccggaga tggcggcgtt ccgcgacgaa ctgttcgggc cggtggcggc ggtcagcaaa
     5881 gcgaacgacg cggaccacgc gttggcgttg gcgaaccatt cggcatacgg cctgtcttcg
     5941 gcggtgctca ccaacaacat gcagctcgcc atgaagttcg ctctcgagct ggaagcaggc
     6001 atggtccacc tgaacggacc cacggtgcac gatgaagcca cggtgccctt cggcggcgtg
     6061 aaagactccg gcatgggccg cgaaggcggc cgctggtcca tcgaagaaat gaccgaagtg
     6121 aaatgggtaa ccattcaaat gggcaagcgg atgtatccgt tctaatccgg attcctgacc
     6181 acccatgcgc ttattgtcat agccggcccc gccgcgcgtg tcatcctaag cgaaggacct
     6241 caaccgctcc acacctcggt gcaaacattc agttgagatt cttcgggctc gacttcgtca
     6301 aacctccagg atgacaggcc cattcttggt cgtcggcact ctcagcaatc gaagaaaacc
     6361 gtttccccgg cgccttgcag aatgacatcc cattcgaggg cgccctttac ggcggtcttt
     6421 ctcgcgatca atgtgccgcg ccgcgcatgc tcaatgagat tcaggatagg atcatcagcg
     6481 ttcgcggatt cctccgggaa atagatccgc gtcaccagcc gcagcagtaa tccccgcgcg
     6541 aatacagata tggcaatgtg aggtgcttgc atctttccat cgggactggg aaccgcacct
     6601 ggctttaccg tcgtgaacgc gaatctaccg ttctcatcaa cccccatccg tccgtagccc
     6661 tggaagcccg gctcgagcgg cttgttttgg ttgtcttcgg gatgcgcgta cttgccatga
     6721 ctgttcgcct gccagatttc gagcagcgcg tctgaaaccg ggttaccatc gccgtcgacc
     6781 acccgacccg cgatggtgac ccgctccccc gaaactcctg ctcccaccag gtcgactttc
     6841 cttagccggg tgagaccgat cgagaagtaa gggcctacgg tttgcgatgt ggttgcctgg
     6901 aggctcatct tatttctcca tcggcgtggt ttcacggccg ctgaggatca cgtcaaagcg
     6961 gtagcccagc gcctgatcgg ggatggtgct cccccaatca aaaacggaaa tcagccggtt
     7021 gcgagcctgc tcatccgcgg tgcaattgaa aatgggatcg taggggataa gagcgtcgcc
     7081 cggaaaatac atttgcgtga cgaggcgcgt ggcaaaggcc ggcccaaaaa ttgagaaatg
     7141 aatgtgcgcc gggcgccagg cgttgtgatg attgcgccat ggatacgccc ccggccggat
     7201 ggtgacgaag cgatagcggc cttcgccgtc tgtgagcgtg tgaccgcagc cggtaaaatt
     7261 cggatccagc ggagcgtcgt gctggtcagc ctcatgcaaa tagcgtcccg cggcattcgc
     7321 ctgccagact tcgacaagcg tatgtggaac cggccgcgcg tcctcatcca ctactcggcc
     7381 gctcacgatg atccgttcgc ccagaggctc gccagcgtgc tgccgggtca gatcgcaagc
     7441 cttcaaatca atcgcttctt ttccaaacaa cggccccgtt acttccgtaa gcgtgtgcgg
     7501 catatacact aacggtctcg atggagcacg tttgacggag gaaatgtacg gagggtggag
     7561 atgcttcggc tgcgtgccag gatccggccg ccgatatccc gcgatgttcg tcatgcgctg
     7621 aatctccttc tcttgcttag aacaatagag cagcgattgt agaccggctc ggctcgttcg
     7681 aaaggccaat ataatagcgt tcgaatcgcc ccaggttcct tcgtgaacag ccaagtacgc
     7741 gcttcttccg aaccgcaaca aaaattcatt cgaacgtcgc ggggccatct aagcaggcca
     7801 agaaaaagaa gtcaaaccga taatcgaaaa gctgtgcgca tatcggccaa attcgtgtat
     7861 tctctcccat tcgaatgacg gagtggaggg ggacgcctat gccggtggcc gattcgaatc
     7921 ccagtgtcag aaaacagtcg cgcacacggt cggtgtccgc cgcattgctg gaatctgtgc
     7981 aatctccttt acctccattt gagggcgcgg gcggtgatcc caacttcatg acttcgctcg
     8041 ccaggggtct tgccgttatt caggcgttct ctcaaacgaa gcgccagcaa accgtttcac
     8101 agatcagcag taagacgggg ttttcgcgcg cagccgtgag gcgttgcctc tacacgctgg
     8161 cgaagttggg cttcgccggt tcagacgaca accgccattt ccatcttcgg ccgcgcgtct
     8221 tgacactcgg gcactcctac atttcatcga tgccacttgc cgtggcggct cagccggttc
     8281 tggagcacgt cagccacgtc ttgcatgagt cttgctcgat tgccaccttg gacggcccgg
     8341 agattgttta tatcgcccgt gcaaccgtga cgcgaatcat gtcgatcgac cttggcgtcg
     8401 ggagccgctt gcccgcgttt tgcacatcga tgggacgaat tctgctcgcg gacttgccgc
     8461 cggaaaaact tgaagagctg cttgcgagca tcgagtttac gcgctacacc gagcgaaccg
     8521 tgaccagcgt ggaaaagctt cgacagattc tgcgaatcgt gcaacgcaac ggctattccg
     8581 tcatcgatca ggaattggaa tacggtctgc gctcgatggc cgtgccgatt cgaaacccaa
     8641 ccggaaaagt ggttgcggcg ctcaacgtcg gcgcgcacgc gcagcgagtg tcgctccagg
     8701 atctgctgat gcgatttctg ccgcacctca aagcggcggc gcaggagctt tgcatgttct
     8761 tgaaataagc gacgcctatc gcaaaacgac ttcagggcaa gccgccgggc attcgatcgg
     8821 gctggtctga aattcaaagc tcagggggga tccggaaggc gcatggacaa ttcaaagaga
     8881 tacgagaaca ccctggttgc gatcctcttc ttctcctggg gcacggtatt tctcgaccgg
     8941 acatcacagc actatctggc tccgtacttc gcgcctgaat ttcatttgag ccaccagcag
     9001 attggcatgt tgggttcggt ggtggcggtt acctgggcat tgtcgacact cttttttggc
     9061 gcgctgtcgg atcgcgtcgg gcgaaagccg atcctcgttc cggtcgtgtt cattttctcg
     9121 atgctgtcct ggatttcggg gatggtgcat agtttccacc agctgctcct ggttcgcgcg
     9181 ctgctggggc ttgcggaagg accggcgtgg tcgatcatga cggcgatgat cgaggaatcg
     9241 tcgcacccga cacggcgcgg gcggaatatc gggatcgtcg taagtgccgc agcgctagtc
     9301 ggattggcgg tcgcgccggt gctcacaacg caggtggcgg cgcgaatggg ctggcgatgg
     9361 gcgttttttg tgtccggagt acccgggttc ttgctagggc tgctgatttg gaaattcgtc
     9421 aaagagccgg agaggggaaa tgctgcggga gctcacggcc aagcggtgaa gattcgagat
     9481 tttctttccg tgctgcgtta ccgcaacatg tggctgtgct gcgcgggatc ggcggcgttc
     9541 ttgtcgtggc tgtttcttca caacactttc gggccgttgt atatcaccga agtggcgcat
     9601 caagcgccca cgaccgccgg attcttgatg ggtgcgagcg gtctcgggag ttttttcctc
     9661 gcactctact ttccccgttt gtccgatcgc attggacgca aaccggttct actggtgatg
     9721 gcgggcatgt gcatgatcgt gccgctcgcg ctgctgatct cgccgctcta cgcgtacccg
     9781 tgggttctgg cggcgattgt ttgtttggca aatgccggcc agggaatcgg ggcgcttatc
     9841 ctggtgctcg tgcctaccga aagcgttccg ccgcaatttg ccgctacggc catcgggctc
     9901 gccacgctgg cggcggaaat tatcggggcg acgctcgcgc caattctggc ggggagagtg
     9961 gcccagtcct acgggctcgg cgccacgatg tggatgtcgg cgggaggagc ggtgctggtt
    10021 tttgtagttg cgttgttttt gaaagagact catttggcaa gagcagggcg ccccgctctg
    10081 caggaagcac ctgcggaatg atcgggagaa gcgatgggcg ctcactcgca aagagatgga
    10141 actgatagaa ctaactgtgg gctgcgccgt ggcgggcgcg atgtttcttt cccaggccga
    10201 catcaagcga aggatggggc gaaggctttc accatgtctc gtaagcgaat actcgacgcg
    10261 cggcggcacc tgagcgtaga ccttgcggcg gatgatctcg tcgcgctcca attctctcag
    10321 ttgctgcgta agcatttttt ggtgggtccc gagaggagct tatggagttc gctgaaccgg
    10381 cgaatcctgc cttgcaggta gtacagaatg gacggtttcc attttccgcc gatgatgtgg
    10441 gtggtgacgg tgacggggca attgaacgga tccattttgg aatcggtcct gataccaggt
    10501 accaataagg tgcctactag caaaaaagat actttagcct aatatcgacc ggccgattca
    10561 cgcaagaaga ttctcgaacg atagccggct gatgaaggag ggcgaggata atgagcaaaa
    10621 ggcccgtagt gctttatggc gccagtggct tttcgggacg cctcatcgcg gaatttcttc
    10681 gcgaatacaa cgtacccttc atcgcggcgg gcaggaacag cggaaagatt caggatgtct
    10741 tga
//
LOCUS       DASYVO010000041         1808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_128469, whole genome shotgun sequence.
ACCESSION   DASYVO010000041 DASYVO010000000
VERSION     DASYVO010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128469"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="VGR03_01475"
     CDS             <1..1512
                     /locus_tag="VGR03_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEV1992978.1"
                     /translation="LDALVQSLGRFSEKLLILGAHARGESVATRSARVGPSLIFQRLW
                     QACSIDKVRTALLLDRRFEFSVERALFLTVLHRLFAPGSDRAAEKWKQDYGMEGSGEL
                     SLHHLYRAMAWLGERLPENRQSGATPFAPRTNKDLIEEELFAQRQDLFSGLDLVFFDT
                     TSIYFEGEGGETIGRRGHSKDHRPDLKQMVVGVVLDQNGNPVCSELWPGNTADVKSLV
                     PIVERLKTRFRIGAVCIVADRGMISAATLAEVEKREWKYILGVRMRSSTEAKAVVGRA
                     GRYAEVHPKSEDPTDPSPLKVKEVWVEDQRRYVVCVNEDQAKKDRHDREAVVASLRDA
                     LKNGDKSLVGNKGYRKFLRAGGRQFAVDEGKIQDEARYDGKWVLTTNTDLPAREVALK
                     YKQLWMVEDVFRSMKSLLDTRPIFHKCDETIRGHVFCSFLALLLRKELEDRLTSKGFD
                     TEWADIVRDLDNLIEMEVTIGGKGYVFRGQTPGVTGKVFQACGVALPPMLRPC"
BASE COUNT          393 a          487 c          595 g          333 t
ORIGIN      
        1 ctcgacgcgc tggtgcagtc gctgggacgc ttctccgaaa agctgttgat cctcggcgct
       61 cacgcccgcg gcgagagcgt ggccacgcgc agcgcccggg tcggtccgtc cctgatcttc
      121 cagcggctct ggcaagcctg ctccatcgac aaggtgcgaa cagctttatt gctggaccgc
      181 cgcttcgagt tttccgtcga gcgggcgctc ttcctcaccg tgttacaccg gctgttcgct
      241 cccggcagcg accgggccgc cgaaaagtgg aaacaggact acggcatgga aggcagcggc
      301 gagctgagcc tgcatcatct ctaccgggcc atggcttggc tcggagaaag gctaccggaa
      361 aatcggcaaa gtggcgcaac gccgttcgcg ccccgaacca acaaggacct catcgaggag
      421 gaattgttcg cgcaacgtca agacctgttc tcaggcctgg atctcgtgtt tttcgacacc
      481 acgtcgatct acttcgaggg agaaggcggc gaaaccatcg ggcggcgggg ccactccaag
      541 gaccatcgac ccgatctaaa gcagatggtc gtgggcgtgg tgctggatca gaacggcaat
      601 ccagtatgca gcgaactctg gcctggcaat acggccgacg tgaaaagtct ggttcccatc
      661 gtggagcggc tgaagacacg gttccggatc ggggcggttt gcatcgtagc cgaccgcggg
      721 atgatcagtg ccgcgacgtt ggcggaagtc gagaagcggg aatggaagta catcctgggc
      781 gtgcgcatga gaagttcgac ggaagccaaa gccgtggtgg ggcgcgccgg acgctacgcc
      841 gaagtgcatc ccaagagcga ggatcccacc gatccctctc ctctcaaggt gaaggaagtc
      901 tgggtggagg atcagcggcg ttatgtcgtc tgcgtcaacg aggatcaggc aaagaaggat
      961 cgccacgacc gcgaggccgt ggtggcctcg ttgcgcgacg ctctgaagaa cggggacaag
     1021 tctctggtgg gcaacaaagg gtatcgcaag tttctgcgcg cgggaggaag gcagttcgcg
     1081 gtggacgagg ggaagatcca ggacgaagcg cgctacgacg gcaagtgggt tttgacgacg
     1141 aacacggatc tgccggcgcg cgaggtggcg ctgaagtaca aacaactctg gatggtggag
     1201 gatgtgttcc gttcgatgaa gtcgctgctg gacacgcggc ccatctttca caagtgcgat
     1261 gaaacgattc gcggtcatgt gttttgctcc tttctggcgc ttttgctgcg caaggaactc
     1321 gaagaccgcc tgaccagcaa gggctttgat acggagtggg ccgacatcgt ccgggatctc
     1381 gacaatctga tcgagatgga ggtcacgatc ggcgggaagg gatacgtttt tcgcggccag
     1441 acgccgggag tgacgggtaa agtttttcag gcgtgcggcg tcgctttgcc gcccatgctg
     1501 cgaccatgct gaactctccc cagtcacgcc caggcggaga aaaagcgtgt cactaggcca
     1561 ttttgaaaat cataacccgt tcagaactga taacttccgg cacagtgctg tagaagatga
     1621 gcctaactgc aacgaaactc tgtcacccct ggcaggctgg agaggtgatc acgttgacac
     1681 aggaagaact gaaacgggtg aaagtagtgg agaacgccgt tatgcgaaga atcacggtga
     1741 tggaggcggc gaagttgttg gacttgagca cgcgccaggt aaaacggctg aagcggcgct
     1801 tcgagtcc
//
LOCUS       DASYVO010000042         5533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_269522, whole genome shotgun sequence.
ACCESSION   DASYVO010000042 DASYVO010000000
VERSION     DASYVO010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269522"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VGR03_01480"
     CDS             <1..364
                     /locus_tag="VGR03_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992979.1"
                     /translation="TWLAACGLEFPEVPGKVIEVVPGTDFILLKIAFVTSDDELEKAT
                     TPNMDVKVVGQPDAARVEKDSAVHFTGTLTSYDPDPAFFLHWEKAKVKEEDIPKGKAA
                     PKKPVHKPAARKPAAKPS"
     gene            complement(462..680)
                     /locus_tag="VGR03_01485"
     CDS             complement(462..680)
                     /locus_tag="VGR03_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1992980.1"
                     /translation="MKDQLEGLVNQMVERGILFDEAVGEFEKRFIKRVLDRTNGNQSR
                     AAEILGIHRNTLSRKIDEYKLDSNGHRR"
     gene            complement(727..1623)
                     /locus_tag="VGR03_01490"
     CDS             complement(727..1623)
                     /locus_tag="VGR03_01490"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV1992981.1"
                     /translation="METQFAGVTPLARGKVRDIYDLDDKLLIVATDRLSAFDVILPTP
                     IPDKGRVLTQLSLFWFHLLKNVIPNHVVSATDFPPPFDQFKDELAGRSMVVQKTQPLP
                     IECVVRGYVSGSGWKDYRVTGKICGIPLPTGLRESDRLPEPIFTPATKAATGHDENIS
                     FERAATLIGKELAEKVRAVSLEIYSRAAAYAELRGIILADTKFEFGILYHRAIDRAAA
                     DDLMWIDEALTPDSSRFWPAAHYSPGGPQASFDKQFVRDYLERMQWPKTPPGPELPPE
                     VVEATRAKYREAYRILAGRELD"
     gene            complement(1782..2204)
                     /locus_tag="VGR03_01495"
     CDS             complement(1782..2204)
                     /locus_tag="VGR03_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992982.1"
                     /translation="MRIAKRLFVQAGVAPKLLAGLKVENLTRTILPPSKHPSLIGSFS
                     ISFDNNESGIVHNLFFIATMEGNRYLTEFSWTQISRGETDNEDLQFIDHADLFGDGKD
                     EVIVGLELYENYFYRIYERKKDSSRWEQIFEVEAPGCD"
     gene            complement(2710..>5533)
                     /locus_tag="VGR03_01500"
     CDS             complement(2710..>5533)
                     /locus_tag="VGR03_01500"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992983.1"
                     /translation="GEQDRLNQRIYELDAEIRQNQNVLNLTALEVDRSENRIAFNRQR
                     AEELAGRHAQVDAELAQATAQAVEWESRSASQIQAVTLLREESGVLTARVEELAKCAE
                     SRAAQIAESEVGIDALRRSSSEAGESLLRLHGEQKQAEEALVHQAEALRKLEANEHDL
                     LETSMRARDDAESAAYDHESATAQLSALRQSATELQAKIAGFCEQRDALSKEAAALRD
                     SLAGVRARHSTLTQILNDRSYTADAVQKLFAANERGGGQDFRAVGVLADYAEVEEQHE
                     AAIEQYLRDELEYVVVETYDHARAGVSMLRDEVGGRATFFVDSLRNLRLSSEYEPIVN
                     FRAEEGVISRLDKLVEFRDPLGAAAKQFLPRLRTAYLTDSTDAAEKLSRENSQYAFVT
                     PDGPCYQGRMVTGGRPDEVGPLGMKRELRALDAEVRQLEHQMSEKQAALQAAATELRS
                     TEQALAEIVEKQREAERRAISAMHRHGQMQSELARLGLELTICQNELSRIRQDVENAR
                     QRAERAKHQHAAAALSRAEAEAESARLAEALVQLRGSIQSDQNELASSRAEFAAMNER
                     LMAAEALAARLNEERAELGRREAALQQQVTSIGDETAALARQSEELALQLEGLRGEKL
                     RLEIRQRELEQEWDAARTRVTQMEDHLRMGRQSLQELREQRSHAEVDRAKNDSDRQHL
                     RETCMSEVNAQPEDLIATEAAFMSGEELAIAETNYREMKQRIESMGPVNMMALEEFNE
                     CEQRFTFLTRERDDLLQSIADTQQAIAELDLATKEKFEHAFHAINKSFSEAFHTIFGG
                     GMAEMRLTEPDSSGDAGIDIVASPPGKRLQNVLLLSGGEKAMTALALLIAIFRYQPSP
                     FCILDEVDAPLDEANVGRFTRLIGEMSGQTQFIIVTHNRKTMEMGSVLYGVTMQEPGV
                     SKLVSVRWEESQAPAPKQQAAAASAA"
BASE COUNT         1030 a         1577 c         1710 g         1216 t
ORIGIN      
        1 gacctggctg gcggcctgcg gactggaatt tcccgaagtg ccgggcaagg tgatcgaagt
       61 ggtgcccggt acggatttca tcttacttaa aattgccttc gtgacgagcg atgacgagtt
      121 ggaaaaagcg actacgccta acatggacgt gaaggtggtg ggccagcccg atgcggcacg
      181 cgtggaaaag gacagtgccg tccactttac cggcacgctg acttcctacg atcccgatcc
      241 ggcgtttttc ctgcactggg agaaggccaa agtgaaggaa gaggacattc cgaaggggaa
      301 ggcggcgccg aagaagccgg tgcacaagcc ggcagcgaga aagccggcag caaaaccgag
      361 ctaatattct cgatctccga cgaagtaatg caaacggctc cccggccatc ccggggagcc
      421 gtttgcattt ttgcaagcag cttggagatt ttttagcgcg cttaccgacg gtggccgttg
      481 gaatcgagct tgtactcatc gatcttgcgg ctcaaggtat tgcggtgaat gccaagaatt
      541 tcggcggcgc gggactggtt gccgttcgtg cggtccagaa cgcgcttgat gaagcgcttt
      601 tcgaattcgc cgacggcttc gtcaaacagg atcccgcgct ccaccatctg attgacaagc
      661 ccttccagtt gatctttcac gatggcacgc tccgctcgaa aacttacggt gtgagggccc
      721 ggcttgctaa tccagttcgc gcccggccag gatgcgatag gcttcccggt atttggcgcg
      781 agtggcttcg acgacttcag gaggaagttc cgggccgggc ggggttttcg gccactgcat
      841 tcgctcgaga tagtcgcgaa caaactgttt gtcgaaagaa gcctggggac cgccgggact
      901 gtaatgcgcg gcgggccaga agcgggaaga atcgggcgtc agggcttcgt caatccacat
      961 caagtcatcg gccgcggcac ggtcgatcgc gcgatggtac agaatgccaa actcgaattt
     1021 tgtgtcggcg aggataatgc cgcgcagttc ggcgtaggcg gcagcgcggg agtagatttc
     1081 caggctgacg gcgcggactt tctcggcaag ctctttgccg atcagtgttg cggcgcgttc
     1141 gaaggagatg ttttcgtcgt ggccggtggc ggcttttgtt gcgggcgtaa agatgggctc
     1201 tggcaaacgg tctgattcgc gcaaccccgt gggcaacgga atgccacaga tttttccggt
     1261 gactcgatag tccttccaac cggagccgga gacgtagccg cgcaccacgc attcaatcgg
     1321 cagcggctgc gtcttttgga cgaccatgga gcggcctgcg agctcatctt tgaattggtc
     1381 gaagggcggg ggaaaatccg tggcgctgac gacgtggttg gggattacat tcttcagcaa
     1441 atggaaccag aaaagcgaaa gctgcgtgag gacgcggccc ttgtcgggaa tgggcgtggg
     1501 aaggatgacg tcgaaggccg agaggcggtc cgtggcgacg atcaggagct tgtcatcgag
     1561 atcgtagatg tcgcggactt tgccgcgcgc gaggggcgtg accccggcga attgcgtttc
     1621 catcaagact ttcatggcag gagcagaaga cacagagtaa gttcccccga caaagtgaga
     1681 tgcattctag cgcagcgcaa cgctatcgcc aagccgtctt cggtgagtct ctctgaaaaa
     1741 ccttttcatt gctccgagca gctgtcgcgc ccaatcggtg gttagtcaca gcccggcgct
     1801 tcgacctcga aaatctgttc ccagcgtgaa ctgtccttct ttcgttcgta gatgcggtaa
     1861 aagtaattct catacaattc gagcccaacg attacctcat cctttccatc accaaagagg
     1921 tcggcatgat cgatgaactg cagatcctcg ttgtcggtct caccgcggga gatctgtgtc
     1981 caggaaaact ccgtcaggta tcggtttccc tccatagtgg cgatgaaaaa caagttgtgc
     2041 acaatgccac tctcattgtt gtcgaaagat atcgagaagg agccgatcag acttgggtgt
     2101 tttgacggag gaaggatggt cctcgtcaga ttctccacct ttaatccagc caacaatttg
     2161 ggcgccacgc ctgcctgaac gaatagcctc tttgcaatgc gcatggccgc attgcgttct
     2221 gttgccgtgg gagattgtct ggagaagatc ccggagcggt gtccagtagc attggttgca
     2281 agcgcctcag tcggtccatc cacgggcgcc aaacctttgt actcaaccgg gtaaggtgcg
     2341 aagtttccat cggccttgag gagtgaggct cttccagccg gtctacctcg gaagaagacg
     2401 ttgtaggaac ggttgggtgt tacatagttc ttccgaaact cttcgtatgc cggatctttg
     2461 tctctgcaac cattggggat tgggagcagt ttcccatcgg aaaccctggc gatgggctcg
     2521 atgatgaatc ctcccgatag gttcgcctga agcgcaaacc agatggcttc attgttacga
     2581 gaaaagtctt cttgttggct gggcctgcta ggaacgatgg cgaaggcagc ggcgacgaga
     2641 caggataatg cgacagagcg caagtcttcc tcaattctcc gccgcgttta catattacat
     2701 caagttaggc taagcggcgg aggcagccgc agcttgctgc ttgggtgcgg gagcctgcga
     2761 ttcttcccag cggacgctga cgagcttgga gactccaggc tcctgcatcg tcacgccata
     2821 aagcacgctg cccatctcca tggtcttgcg gttgtgggta acgatgatga actgtgtctg
     2881 gccgctcatc tcgccgatca ggcgcgtaaa gcgcccgacg ttggcttcat cgagcggcgc
     2941 gtcgacttcg tcaaggatgc agaacgggct cggctggtag cggaagatgg cgatgagcaa
     3001 cgccagcgcg gtcatggcct tttcgccgcc ggagagtagc aacacgtttt gcaggcgctt
     3061 gcctggaggt gatgcgacaa tgtcgatgcc tgcgtcgccg gagctgtccg gctccgtcaa
     3121 gcgcatctcc gccatgccgc cgccgaaaat agtgtggaag gcttcggaga agctcttgtt
     3181 gatggcgtgg aaggcgtgct cgaacttttc cttggtggcc aggtcaagct cggcgatggc
     3241 ttgctgggtg tccgcaatcg attgcaacag atcgtcgcgc tcgcgcgtga ggaacgtgaa
     3301 gcgctgctcg cactcgttga actcttcaag cgccatcatg ttgaccgggc ccatggactc
     3361 gatgcgctgc ttcatttcgc gatagttggt ttcggcgatg gccagctctt cgccggacat
     3421 gaaggccgct tccgtggcga tcaagtcctc gggctgggca ttcacttcac tcatgcacgt
     3481 ttcgcgcagg tgctgccggt cggaatcgtt cttggcgcgg tcgacttcgg cgtggctgcg
     3541 ttgttcgcga agttcctgca acgactggcg gcccatgcgc agatgatctt ccatctgggt
     3601 aacgcgcgtg cgggcggcgt cccattcttg ctcgagttcc cgctggcgga tttccaggcg
     3661 caacttttcg ccgcgcagac cttcgagctg cagggcgagc tcctcgcttt gcctcgcgag
     3721 ggcggccgtc tcgtcgccga tggaagtcac ttgctgctga agcgcggctt cgcggcgtcc
     3781 gagttcggcg cgctcttcgt tcagccgagc ggcgagggct tcggcggcca tgagccgctc
     3841 attcatcgcg gcgaattcag cgcggctgga ggcaagttca ttctggtccg attggatgga
     3901 accgcgaagc tggacgagcg cctccgcaag ccgtgcgctt tcggcttcgg cttcggcgcg
     3961 gctcagagcg gcggcggcat gctggtgctt cgcgcgctcg gcacgctgcc gagcgttctc
     4021 cacgtcctgg cggatacgcg agagctcgtt ctggcagatg gtgagttcca gcccgagtcg
     4081 ggccagttca ctctgcatct ggccgtggcg gtgcatggcg gagatggctc ggcgttccgc
     4141 ttcgcgctgc ttctcaacaa tttctgcaag cgcttgttcc gtcgaacgca attcggtagc
     4201 ggcggcctgg agcgcggcct gtttttcgct catttgatgc tcgagctgcc tcacttccgc
     4261 gtccagcgcc cgcaattcgc gcttcatgcc cagcgggccc acttcgtcgg ggcggccgcc
     4321 tgtgaccatg cggccctggt agcaagggcc gtcgggcgtg acgaaagcgt attgcgaatt
     4381 ttctcgcgac aatttttccg ccgcatccgt gctgtccgtc aaatacgcag tgcgaagccg
     4441 tggcaggaat tgtttcgccg ccgcaccaag cggatcgcga aattccacca gcttgtcgag
     4501 ccgcgaaatg acgccctctt cggcccggaa attcacgatc ggctcgtatt cggaggagag
     4561 acgcaggttg cggagcgaat ccacaaagaa agttgcccgg ccacccactt catcgcgcag
     4621 cattgaaacg ccggcgcgcg cgtggtcgta ggtctcgacg acgacatatt ccagctcgtc
     4681 gcgcaagtat tgttcgatgg cggcttcgtg ctgctcctcg acttcagcgt aatcggccag
     4741 cacgccgacg gcgcgaaaat cctgcccgcc gccgcgttcg ttggcggcaa aaagtttctg
     4801 caccgcgtcc gccgtgtagg agcggtcgtt cagaatttgc gtgagcgtcg aatgtcgggc
     4861 gcgaaccccc gccaacgaat cgcggagcgc ggcggcctct ttcgacagag cgtcacgttg
     4921 ctcgcagaat ccggcgatct tggcctgaag ctccgtggcg ctttgtctca acgcactgag
     4981 ttgggccgta gcggattcat gatcgtaagc ggcgctctcg gcatcatcgc gcgcgcgcat
     5041 cgaagtttcc agcaagtcgt gctcgttcgc ttcgagcttc cgcagcgctt cggcctggtg
     5101 tactagcgct tcttcggctt gcttctgctc gccatgcaga cgcagcagac tttcgccggc
     5161 ttccgatgag gagcggcgta gcgcgtctat ccccacttcg gattcggcaa tctgggcagc
     5221 gcgagactcg gcgcatttcg cgagctcctc gacgcgagcg gtcaaaacgc ccgattcttc
     5281 gcgtagcagc gtaaccgcct gaatttgcga agcacttcgt gattcccatt ccacggcctg
     5341 tgccgtggct tgcgccagtt ccgcatcaac ctgggcatga cgcccggcga gctcttcggc
     5401 gcgctgccgg ttgaacgcga tgcgattttc actgcggtcc acttcgagtg ccgtaagatt
     5461 caaaacattc tggttctgcc ggatctcggc atccagttcg taaatccgtt ggttcagccg
     5521 gtcctgttcg cct
//
LOCUS       DASYVO010000043        11916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_255491, whole genome shotgun sequence.
ACCESSION   DASYVO010000043 DASYVO010000000
VERSION     DASYVO010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 11916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11916
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255491"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..1071
                     /gene="pyrH"
                     /locus_tag="VGR03_01505"
     CDS             313..1071
                     /gene="pyrH"
                     /locus_tag="VGR03_01505"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV1992984.1"
                     /translation="MAATKKAGKKAVKKAKPAYRRILLKLSGEAFQGPQGFGIHGETI
                     QAIARELKDVHQLGVQTAIMVGGGNIFRGARQKGFEIDRATGDYMGMLATVINALALQ
                     DALEKEGVFTRVLSAIEMKEVSEPFIRRRAVRHLEKDRVVIFAAGTGNPYFSTDTAAA
                     LRAMEIKADVILKATRVDGVYDADPEQIPDAKFFAAISYREVLSQNLKVMDSTAISLC
                     MDNGMPIVVFNMNRPGNIKRVVLGERVGSKVTPA"
     gene            1230..1793
                     /gene="frr"
                     /locus_tag="VGR03_01510"
     CDS             1230..1793
                     /gene="frr"
                     /locus_tag="VGR03_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV1992985.1"
                     /translation="MTTKDAIAAGRTRMEKAVEDFRKELGTLRTGRANASILDAIRVD
                     YHGTPMPVNQLGTVTVPEATLLVISPWDPSVVPLIDKAIRTSDLGLNPTNDGKVVRVP
                     MPSPTEERRKEIVKHLHKVLENHRTAVRNIRRDVKETIDKLEKDKKISQDEQKRALDE
                     MEKISHAETKKIEDLSGAKEREILELK"
     gene            complement(2506..4578)
                     /locus_tag="VGR03_01515"
     CDS             complement(2506..4578)
                     /locus_tag="VGR03_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEV1992986.1"
                     /translation="MRLPLRFHPLLRALTLSFLLASAIFASTQAQQAPQKPPEKPSAK
                     ASTPTEKAENPAQIELLETRYRFEANGDSRKEVHARVHINSELGVRQFARLNFDYNRS
                     FENVEIPLVRITHPSGGTADILPSAITDQPNPAVVNAPAYQDVRVKSVRILGLAPGDD
                     LEYRVITTVAHHPLAPDFWLDHSFDRTGVVTQEIFELDLPGHLNSTATMGNWHGGPAL
                     VVYLTVQETSYRYDPSQGSSGRSIYRWEILSTRRLPAPIDDDGAPAPDVLVSSFPDWH
                     ELVTRLAPHFFNWPEDDRKEAEARLHSFAPKAGTPDKKLRAGYEFVSQKLATVDLPVG
                     ATGFHLRHVKEILDSGYATSEEKCSLLAQFASVAGIKAEIVFVGAPETQKQFARPSPF
                     NHVFVIVPEKKKHIALDPSLEVAPFGLIAPQFRGKTALSLTLYDGGDYMSVNWSSLLA
                     ELPFSPSQRVSIDATLSAGGTLSSKVHYTLRGDNELLLRVAFHQAPKEKWKDLAQLLS
                     LSDGFRGQVTSVNASDPYATHEPFTVDYEIAQPKFVDWSKKPVRILALLPQLGLPDPP
                     AKPTAGAAPSPIELGTPLEVETQMTLHLPPGTTPRTPTGTSVQRDYATFASQYSAKGL
                     TVTASRHIHFLLRQVPAESAADYNAFLRAVLNDEAQDFTLDRPGAPALDVKPAAPTAA
                     APQPTPKP"
     gene            4898..6451
                     /locus_tag="VGR03_01520"
     CDS             4898..6451
                     /locus_tag="VGR03_01520"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEV1992987.1"
                     /translation="MRCVLAGVAALVVGISLGGAWTARADNKPAPKGPDEDGTKPALV
                     KIAGEGIMDSHAFQYLTELSDDIGSRVTGSPAERKAEEWGVAKMKAIGLENVHTEKYQ
                     LWRGWTRGTAQGELLEPIRRALHVDAMGWTGSTPAGGAEGEVVTANLFDIEEEVKNAS
                     RLSGKIVLVIMRGTPKKDEDSLFAIFGDFLKAASKAGAIAVIGGQAGAKASGMNLTHT
                     GILGFDADFAIPVLSMTAEDHGQLERYVESGKKVRVRFNVQNTFSNGPVESANVVGEI
                     RGRENPEQVLVVGAHLDSWDLSEGTTDNGTGSASVLASAEAIVRSGMKPRRTIRFVLF
                     TGEEQGLNGSFAYTKHHQSEMANHLGNLVLDNGQGPVKEFMLGGRDDLVANFRPFAES
                     LSSIRNIPVNDKVENGTDTLPFSMAGLPGINMDQDSPDYKYTHHSSADALEAVKPEVL
                     AQNATLMALTAYWIADRPERFASPWTAEKTAKMLRAQHQYEMLNAFHLWPFGDLGSED
                     KGRNDRPQN"
     gene            7113..7499
                     /gene="rpsL"
                     /locus_tag="VGR03_01525"
     CDS             7113..7499
                     /gene="rpsL"
                     /locus_tag="VGR03_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV1992988.1"
                     /translation="MPTFSQLVRMGREAVRVKTKSPALAGCPEKRGVCIRVYTQTPKK
                     PNSALRKVARVRLTNAIEVTTYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGTL
                     DAAGVENRKQGRSKYGAKRPKAAAAK"
     gene            7555..8025
                     /gene="rpsG"
                     /locus_tag="VGR03_01530"
     CDS             7555..8025
                     /gene="rpsG"
                     /locus_tag="VGR03_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV1992989.1"
                     /translation="MPRKGLVFRRPQAIDPVYASDIVEKFICSMMWEGKKSTAQRVFY
                     GAMDQVAKKTNDDALKIFKKAIENVKPLLEVKTRRVGGANYQVPVEVNPFRRQSLAIR
                     WLLQYSRERAGKTMVDKLADELIDAANARGGAMKKKEDVHRMAEANKAFAHYRW"
     gene            8062..10326
                     /gene="fusA"
                     /locus_tag="VGR03_01535"
     CDS             8062..10326
                     /gene="fusA"
                     /locus_tag="VGR03_01535"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV1992990.1"
                     /translation="MPRVFKLEDTRNIGIMAHIDAGKTTSTERILFYTGVTYKIGSVD
                     EGTATMDWMEQERERGITITSAATTASWDRFDKKYRVNIIDTPGHVDFTVEVERSLRV
                     LDGAVCVFDSVAGVQPQSETVWRQADKYRVPRICFVNKMDRMGADFDHVIRTIRQRLG
                     AHPLPLQFPLGREDNFIGIIDFLEEKVIVWLEETLGAKFETFPVEKLWDKAFVDSRPD
                     VAGALKGSAIDKKFYDDHRKKVIEYIAEHDDEVIDKYLNEVPLTVEEMRKSIRKSTLA
                     LKIVPVLAGSAFKNKGIQPLLDAVVDYLPSPVDVPPLEGVNPANDESELRLARDDQPF
                     AALAFKIMSDPFVGHVTYIRVYSGVLKSGSYVMNSGKGTRERISRLLQMHANKREEID
                     EIYAGDICACVGLKSVNTGDTLCDEEKQIILENIDFPAPVISVAIEPKTKADQDKLGV
                     AMQRLAQEDPTFRVSTEPDTGQTLISGMGELHLEIIVDRMLREYNVQANVGRPQVAYR
                     ETIRKKATAEGKYIKQTGGRGQYGHCEIELHPLPSSSRENMKEMSTDDLDALAKQIVG
                     GPAGKWKFDKEHRFLFIDKIVGGSIPREFIAPIEAGIREALDNGILAGFTMVDVAAVL
                     IDGSSHDVDSSEMAFKIAGSMAFKSACHKASPVLLEPIMKVEVVVPEEYMPQVFGDLN
                     ARRSAIQGTENRAGSNVIRVEVPLSQMFGYATDLRSRTQGRATFTMHFSHYAEAPASI
                     AQEVIEKATGKVAR"
     gene            10397..11584
                     /gene="tuf"
                     /locus_tag="VGR03_01540"
     CDS             10397..11584
                     /gene="tuf"
                     /locus_tag="VGR03_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1992991.1"
                     /translation="MAKEKFERNKPHVNIGTIGHIDHGKTTLTAAITKVLSKHNPKVQ
                     FRAFDSIDNAPEERERGITIAVSHVEYETANRHYAHMDCPGHADYIKNMITGAAQMDG
                     AILVVAATDGPMPQTREHILLARQVGVPAVVVFLNKCDMVEDAELLELVELEVRELLK
                     SYQYPGDTIAVVKGSALQALNGDAKWEKTIEELMEAVDKNVPLPVRDIDKPFAMPIED
                     IFSISGRGTVVTGRIERGKVKVGEEIEIVGFRATQKKVVTGVEMFKKLLDEGLAGDNV
                     GLLLRGTEKEDVERGQVVCKPGSITPHTKFKAEAYVLTKEEGGRHTPFFSGYRPQFYF
                     RTTDVTGDMKLPTGVEMVMPGDNVSCEVTLITPVALEKGLRFAIREGGHTVGAGAVTE
                     VIE"
     gene            11633..11782
                     /gene="rpmG"
                     /locus_tag="VGR03_01545"
     CDS             11633..11782
                     /gene="rpmG"
                     /locus_tag="VGR03_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1992992.1"
                     /translation="MREIITLQCGDCKNRNYTTTKNKKKHTERLETKKFCNTCRKQTA
                     HKEVK"
     gene            11842..11916
                     /locus_tag="VGR03_01550"
     tRNA            11842..11916
                     /locus_tag="VGR03_01550"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11875..11877,aa:Trp,seq:cca)
BASE COUNT         2723 a         2895 c         3958 g         2340 t
ORIGIN      
        1 cagcactaca tcaaggacga gagcgtgacc atcggcgaga tgatcgccca ggccgtcatc
       61 aatctgggcg agaacatctc gatccggcgc ttcgtgcggt tcaaagtggg agaagtaagc
      121 ggcgcagcgg cggggccgga aacggcgtca gcacctgtga ctgcgtaaag aacaaaccct
      181 ttcgattaca actttacaga gcgtcccggg ttgaccgggg cgctcttttt gttttccggg
      241 tcttcgcaga gtgcgggggc ggagatagtt tgttacaatt cccgtgattt cagatcgcgc
      301 gaacggagac tcatggcagc gacgaagaaa gctggtaaga aggccgtgaa aaaagccaag
      361 ccggcatacc ggaggatcct gctgaagctt tccggagagg cgtttcaagg gcctcaggga
      421 ttcggcatcc acggcgaaac gattcaggcc atcgcacgtg aactcaaaga cgtgcaccaa
      481 ctgggtgtgc agacggcaat catggtcggc ggagggaata ttttccgcgg cgccaggcag
      541 aaaggctttg agatcgaccg cgcgaccggc gactacatgg gcatgctcgc gacagtcatt
      601 aacgcgctgg cgctgcagga cgcgctcgaa aaagaaggcg tcttcacgcg cgtgctgagc
      661 gccatcgaaa tgaaagaggt ttccgaaccg tttatccggc ggcgcgcggt gcggcacctg
      721 gagaaggatc gcgtggtgat ttttgcggcg gggacgggga atccctattt ttcgacggat
      781 acggcggcgg cgctgcgcgc gatggaaatc aaggcggacg tcatcttgaa ggcgacgcgc
      841 gtggacgggg tgtacgacgc cgatccggaa cagattccgg acgcgaaatt ctttgccgcg
      901 atttcttacc gcgaggtatt gagccagaac ctgaaggtga tggactcgac ggcgatttcg
      961 ctgtgcatgg acaacggcat gccgatcgtg gtgttcaata tgaaccggcc ggggaatatc
     1021 aaacgggtgg tgctggggga gcgcgtgggc tcgaaggtga cgccggcgtg agaaagtttt
     1081 cagctctcag tggtcagttt tcagcgggag aagaaaactg aattatgtcc gagtactatt
     1141 cctccagtga gttttctttt tactaagaac tgagcactaa aacggaagtc cggatcatcc
     1201 ggaaagaaaa ttgtttcgat gaggaagata tgacgacgaa agatgcgatt gccgcaggca
     1261 ggacgcggat ggaaaaggcg gtcgaggatt ttcgcaagga actggggacg ctgcgcacgg
     1321 ggcgcgccaa tgcttccatt ctggacgcca tccgcgtgga ctatcatggg acgcccatgc
     1381 cggtgaatca actcggcacg gtgaccgtgc cggaggccac gctgctggtg atttcgccgt
     1441 gggacccgag cgtggtgccg ttgatcgaca aagccatccg gacttcggac ctggggctga
     1501 accccacaaa cgacggcaag gtggtgcgcg tgccgatgcc ttcgccgacc gaagagcgcc
     1561 gcaaagagat cgtgaaacac ttgcacaagg ttctggagaa tcaccgcacg gcggtgcgca
     1621 acatccgccg cgacgtgaag gaaacgattg acaagctgga gaaagacaaa aagataagcc
     1681 aggacgaaca gaagcgggcg ctggacgaga tggaaaagat ttcgcacgcg gagaccaaga
     1741 agatcgagga tttgtccggc gccaaagagc gggagattct cgagctcaag tagttgtcag
     1801 ttgttagttc taagttttta accgagagca agatacaagc cggatacgca ctcctcgccg
     1861 ggggttgtca gtggttacct gcgaactctc ctgcaatgga gccctgcttc gaagaagtcc
     1921 tcgtagcccg ctcaagagtc gttgctgatt gagatggaag ttcaagccgt acttgaagtg
     1981 aggcccctcc catccccccg tgtttttcat aagtgttgct tctaaaggga gttaaagtcc
     2041 tttagaatga acacttctat aagtgttgat tctaaagaga cttacgttgc accaaaattg
     2101 tgcaaaatgg gcttcttcct cctttttttt catgagtctt gattctaaag ggttttaagt
     2161 tgtttagaat gaacacttct gtagcctctg taagtgttga ttctaaaggg acttaggttg
     2221 caccagaatt gtgcaactgc tttgtctgct tgccgtggga ggacggaccc ctgggcggag
     2281 gctacgggat tatcaacaga tatacctaat gtttagaata ccagagaagc agcagtctgt
     2341 caagagaaaa gaaccggcgt gtgtcaagcc tctttaaaaa tgtccctttt cttacctcga
     2401 ttaaagtgtc ccctcggggg gatgggaagg gcttggcgag ggagagcggg cggccccccg
     2461 aaccgggtcg gggccggcgc caggccgccc ctacgggacc gctgttcagg gtttcggcgt
     2521 tggctggggt gcagccgcgg tgggggcggc aggcttgaca tctagcgcag gtgcgccagg
     2581 acggtcgagg gtgaagtcct gggcttcgtc gttgaggacc gcgcgaagga aagcgttgta
     2641 atcggcggcg ctctcggcgg gaacttgcct caacaggaaa tggatgtggc gggaggcggt
     2701 gacggtcagt cctttcgcgg agtattgcga ggcgaaggtg gcgtaatcgc gctggacgga
     2761 agtgccggtc ggggtgcgag gggtagttcc cggcgggaga tgcagggtca tctgggtttc
     2821 gacttcgagc ggcgtgccga gttcgatggg ggacggtgca gcgcctgcgg tgggtttcgc
     2881 cggcggatca ggcaagccta gctggggaag cagcgcgagg atgcgcacgg gcttcttcga
     2941 ccagtccaca aatttgggct gggcgatttc gtagtcgact gtaaacggct catgcgtggc
     3001 atacggatcg gacgcgttca cactggtcac ttgtccacgg aagccgtcgg agagcgagag
     3061 aagctgggcg agatccttcc atttttcttt cggcgcctgg tgaaaggcga cgcgtaagag
     3121 cagttcattg tctccgcgca gcgtgtagtg caccttcgaa ctcagggttc ctcccgccga
     3181 taaagtcgca tcaatgctga cgcgctggga aggggaaaag ggaagctctg ccaggagtga
     3241 agaccagttc acactcatgt agtcgcctcc gtcatacaga gtaagactaa gcgcagtttt
     3301 tccgcgaaac tgaggcgcaa tgagaccaaa tggagcaact tccaagctcg gatcgagagc
     3361 aatgtgcttc tttttttctg gcacgattac gaaaacatga ttgaacgggg acgggcgagc
     3421 gaactgtttt tgtgtctcgg gtgcgccaac aaaaacgatc tctgccttaa tcccggcgac
     3481 gctcgcgaac tgcgcgagca aagaacactt ttcttccgaa gtcgcgtagc cggagtcgag
     3541 gatttccttg acgtggcgca ggtgaaatcc cgtggcaccc actggcaggt cgacggttgc
     3601 cagcttttgt gagacgaact catagcctgc gcgcagcttt ttgtccggag ttccggcctt
     3661 gggagcaaaa gaatgcagtc gggcctcagc ctccttgcga tcatcctccg gccagttgaa
     3721 aaagtgaggc gccagacgcg ttaccaactc gtgccaatcc gggaagcttg agacgagtac
     3781 gtcaggtgct ggtgcgccgt cgtcatcgat aggcgcgggg agtcttcgcg tcgaaagaat
     3841 ttcccagcga tagatggacc ggccggaact gccttggctg gggtcgtatc gatacgatgt
     3901 ttcctgaacc gtgagataaa cgacgagcgc aggaccgcca tgccaatttc ccattgtggc
     3961 ggttgagttc agatggcccg gtaggtcgag ctcaaagatt tcctgagtga cgacgccggt
     4021 gcggtcgaag gagtggtcga gccagaagtc gggggcgagc gggtggtgtg cgacagtggt
     4081 gatgacgcgg tattcgaggt cgtcgccggg agcgaggccg aggatgcgga cggacttgac
     4141 gcgaacgtcc tgatacgcag gggcgttgac gacggcgggg ttgggttggt cggtgatggc
     4201 gctgggaagg atgtcggcgg tgccgccgct gggatgggtg atgcggacca aagggatttc
     4261 gacattttcg aaggagcggt tgtagtcgaa gttgaggcga gcgaactggc gaacgccgag
     4321 ttcgctgttg atgtgcacgc gggcgtggac ttccttgcgg ctatcgccgt tggcttcgaa
     4381 gcggtagcgg gtttcgagta gctcgatttg ggcggggttt tccgctttct cggtgggagt
     4441 ggaggctttc gcgctgggtt tctcgggcgg cttttgtgga gcttgctgcg cttgcgtgga
     4501 agcaaaaata gcggaggcga ggagaaaaga tagggtgagt gcgcgcaaga gcggatggaa
     4561 cctgagagga agtcgcaaga tggcctcgga ggaagtgtag ggcggggacc gaggtgcgta
     4621 aagacacggc tgcgcccgcg ttgccggata tgcaccgcag ggcaagcacg cgccggtcga
     4681 aaaggaaaaa gaaggtaaaa cgcagagaca cagagtgcgc agagaagagg ggggagaggt
     4741 attggcaatc tttttggccg aggggcagga gagaggaatc aagacggaga tttcatgaga
     4801 gacagaaggg aggagaggtt gaagaaaccg aatggcggcg ggtgcgtctg agtgcgtgat
     4861 agagtttgtc tgcgcgagtg gcgcaaggag aatgcggatg cgatgtgtct tggcgggagt
     4921 ggcggcactg gtagtgggaa tttctttggg cggagcgtgg acggcgcggg ctgacaacaa
     4981 gccagcgccg aaggggcctg acgaggatgg gacgaagccg gcactcgtga agattgcggg
     5041 cgaagggatc atggattcgc acgcctttca atacctcacc gagctgagcg acgacattgg
     5101 ctcgcgtgtg acggggtcgc ccgcagagag aaaagcagaa gagtggggcg tggcaaagat
     5161 gaaggccatc ggcctcgaaa acgtacacac ggaaaaatac cagctttggc gcggatggac
     5221 gcgcggcacg gcgcaaggag aattgctcga accgattcgc agggcgctgc atgtggatgc
     5281 gatgggctgg acgggatcga cgcctgcggg aggcgcggaa ggcgaagtcg tgaccgcgaa
     5341 tctcttcgac atcgaggaag aagtaaaaaa tgcttcacgg ctttccggga agattgtgct
     5401 ggtaatcatg cggggcacgc caaagaaaga tgaagattcg ttgtttgcca tctttggaga
     5461 tttcctgaag gcggccagca aagcaggagc gatcgcggtg atcggaggcc aggcgggagc
     5521 gaaggcctcg ggaatgaact tgacgcatac ggggattctc ggtttcgacg ccgactttgc
     5581 catcccggtc ttgtcgatga ccgccgagga tcatgggcag ctcgaacggt acgtcgagag
     5641 cgggaaaaaa gtgcgggtgc gcttcaacgt gcaaaacaca ttttcaaacg ggccggtgga
     5701 atcggcgaat gtggtgggag agattcgcgg acgcgaaaac ccggagcagg tgctggtcgt
     5761 cggagctcat ttggattcct gggatctttc ggagggaacc acggacaacg gcacaggctc
     5821 ggcaagcgtg ctggcttcgg cggaagcgat tgtgcgctcc ggcatgaagc cgcggcggac
     5881 gattcgcttt gtgctgttca cgggtgagga gcaaggactc aacggctcgt ttgcctatac
     5941 gaagcatcat caatcggaga tggccaatca tttgggaaac ctggtgctgg ataacgggca
     6001 agggccggtg aaggagttca tgctgggagg ccgcgacgac ctggtggcga atttccggcc
     6061 tttcgccgag tcgctttcct cgattcggaa tattcctgtg aacgacaagg tagaaaacgg
     6121 gaccgacacg ctgcccttct caatggcggg actaccggga atcaacatgg atcaagattc
     6181 gccggattac aaatacacgc accactcgtc ggccgacgcg ctggaagcgg tgaagccgga
     6241 agtgctggcg cagaatgcca cgctgatggc gctgacggcg tattggattg ccgaccggcc
     6301 ggagcgtttt gcttcgccat ggactgcgga aaagaccgcc aagatgctcc gggcgcagca
     6361 ccagtacgag atgctgaacg ccttccatct ttggcccttc ggggacttgg gaagcgagga
     6421 caagggcagg aacgatcgcc cgcaaaatta gcgtattagg atgcgaagag ggagtagctg
     6481 acgatcggaa gggaactcgc ctggtctcct tgggatttaa gtaactctaa gaatataaat
     6541 gacttagagg ggaaacatgg ttatctaagg cctacggaca ttcctccaag tgctctggga
     6601 tttcgcttca ggtgacatcc gaaaggggcc ggatgtgata aactgtttgg gtagagccgc
     6661 caaggcgatc ggtcagggtg ggcaaagcaa gggccaaaac cccaatattg cataaagttt
     6721 tccactgcct tgtatcgaaa gatttgacag aacgggtttt cttccctata cttcggtgtt
     6781 tgtcagggat gcttttaccc tgagtgggac ggctccagct tgtttggagt cgttgagttg
     6841 gaagggaaat atcttcggtc ccggaacaaa gtaagacgga atgggacaga agagcggccg
     6901 gaatggggcg ccagccccgc aaagccgagt aagcctctcc gaactgaagc caagagagta
     6961 gttctacttc ttggagtgcg tacggagttt cttgctggtt gtatctccgg attcgcagta
     7021 gacctttcag cactagactg cgctctcgcg ccattttgtg tgcgtgcctt gcgggagccg
     7081 cgaaaaatta cgcgcgcgat agagggatga ctgtgccgac gttttcgcaa ctggtaagaa
     7141 tgggacgtga agcggtccgg gttaaaacga agtcgcccgc gctggcgggc tgcccggaga
     7201 agcggggcgt gtgcattcgc gtgtacacgc aaacgccgaa gaaaccgaac tcagcgctgc
     7261 gcaaagtagc gcgcgttcgc ttgacgaatg cgattgaagt gacgacttat attcccggcg
     7321 tcggccacaa cctgcaggag cactccatcg tgctggtgcg cggcggccgc gtgaaggatc
     7381 tgccgggcgt gcgttatcac atcgtgcgcg gaacgctgga cgccgcgggc gtggagaacc
     7441 gcaagcaggg acgttccaag tacggcgcga agcggcccaa ggccgcggcg gcgaagtaag
     7501 gcaaccgttt agcggttgcg cgtgaaacga ttttcgaagg agcgataaag agcaatgcca
     7561 cgcaaaggac ttgttttccg gcgaccgcaa gcgatcgatc cggtctatgc cagcgacatt
     7621 gtggagaagt tcatctgctc gatgatgtgg gaaggcaaga agtcgacggc gcagcgcgtg
     7681 ttttacggcg cgatggatca ggtggcgaag aagaccaacg acgatgcgtt gaagattttc
     7741 aagaaggcca tcgagaacgt gaagccattg ctggaagtga agacgcggcg cgtgggcggc
     7801 gcgaactacc aggttccggt ggaagtgaat ccgttccgca ggcaatcgct ggcgattcgc
     7861 tggctgctgc agtattcgcg ggagcgcgcg ggcaagacga tggtggacaa gctcgcggat
     7921 gagttgatcg atgcggcgaa tgcgcgtggc ggggcgatga agaagaagga agacgtgcac
     7981 cgcatggcgg aagccaacaa ggcgttcgcg cattatcgct ggtagtcggt tcataaatta
     8041 aaggatttct taaaagagat tatgccccgg gtattcaaac tcgaagacac gcgcaacatc
     8101 gggatcatgg cgcacatcga cgccggaaag acgacgtcga cggagcgcat cctgttctat
     8161 acgggtgtga cctacaagat cggttcggtg gatgaaggca cggccaccat ggactggatg
     8221 gagcaggagc gcgagcgcgg catcacgatt acgagcgcgg cgacgaccgc ttcgtgggat
     8281 cgcttcgata agaaataccg cgtcaatatt atcgatacgc ccggccacgt ggacttcacg
     8341 gtggaagtag agcgctcgct gcgcgttttg gacggcgcgg tatgcgtgtt tgactccgtc
     8401 gccggcgtgc agccgcaatc ggaaacggta tggcggcagg cggacaagta tcgcgtcccg
     8461 cgcatctgtt tcgtgaacaa gatggaccgc atgggcgcgg acttcgacca cgtcatccgc
     8521 accattcgcc agcgcctcgg tgcccacccc ctacctttgc aattccccct cggacgtgaa
     8581 gataatttca tcggcatcat cgatttcctc gaggaaaaag taattgtatg gctggaagaa
     8641 actctcggcg cgaaattcga gacatttccg gtcgagaagc tctgggacaa ggcgttcgtg
     8701 gattcgcggc cggatgttgc cggcgccttg aaaggctcgg caatcgacaa gaagttctac
     8761 gacgaccatc gcaagaaagt cattgagtat atcgccgagc acgacgacga agtgatcgac
     8821 aagtatttga acgaagtgcc tctgacagtg gaagagatgc gcaagtcgat ccgcaaatcg
     8881 accctggcct tgaagattgt gccggtgctg gcgggctccg cgttcaagaa taaggggatt
     8941 cagcccctgc tcgatgccgt tgtggattat ctgccgtcgc ccgtggatgt tcctccgctc
     9001 gagggcgtta acccggcgaa tgatgaaagc gaactgcgcc tggcccgcga cgatcagcct
     9061 ttcgcagcct tggcgttcaa gatcatgtcc gacccgttcg tgggccacgt gacgtatatc
     9121 cgcgtgtatt ccggcgtgct gaaatccgga agttacgtga tgaactccgg caaaggcacg
     9181 cgcgagcgca tcagccgatt gctgcagatg cacgcgaaca agcgcgaaga gattgacgag
     9241 atttatgccg gcgacatttg cgcctgcgtg ggtctgaaga gcgtcaatac cggcgacacg
     9301 ctttgcgacg aagaaaagca gatcattctc gaaaatatcg atttccccgc gccggttatt
     9361 tccgtggcca tcgagccgaa aacgaaagcc gaccaggaca aactgggcgt ggccatgcag
     9421 cggctggcgc aggaagatcc cacgttccgc gtttccaccg aaccagatac cgggcagacg
     9481 ctaatttccg gcatgggcga attgcacctg gaaattatcg tggaccgcat gctgcgcgaa
     9541 tacaacgtgc aggctaacgt gggccgtcct caggtggcgt accgcgaaac gattcgcaag
     9601 aaggctaccg cggaaggcaa atacatcaag cagaccggtg gccgcggcca atacggacat
     9661 tgcgagattg agttgcatcc gcttcccagc tccagccgcg agaacatgaa agaaatgtcc
     9721 acggacgatc tggacgcgct ggccaagcaa atcgtgggcg ggccggctgg aaagtggaag
     9781 ttcgacaagg agcaccgctt cctgttcatc gacaagatcg tcggcgggtc gattccgcgc
     9841 gagttcatcg cgccgatcga agcgggcatc cgcgaagcat tggataacgg catcctggcg
     9901 ggcttcacga tggtagacgt cgccgcggtt ctgattgacg gcagttctca cgacgtggac
     9961 agctccgaaa tggcgttcaa aattgcgggc tcgatggcct tcaaatcagc ctgccacaag
    10021 gcctcgccgg ttctcctcga acccattatg aaagtggaag tggtggttcc ggaagagtac
    10081 atgccgcagg tgttcggcga cttgaatgcg cggcgttcgg cgattcaggg cacggaaaac
    10141 cgtgcgggta gcaacgttat tcgcgtggaa gtgccgcttt cgcagatgtt cggctacgcc
    10201 acggatttgc gcagccgcac gcagggccgc gccacgttca cgatgcattt ttcgcattat
    10261 gccgaggcgc cggcttcgat cgctcaggaa gtgatcgaga aggccaccgg caaggtggcc
    10321 aggtaagacg gggttttgcg gtcagggcct tgtaacaagg tatttgaaag acttcgactg
    10381 aggagcagag ggcgaaatgg cgaaggagaa attcgagcgc aacaagccgc atgtaaacat
    10441 tgggacgatt ggtcacattg accatggcaa gacgacgttg acggcggcga tcaccaaggt
    10501 gctgagcaag cacaacccga aagtgcagtt ccgggcgttt gattcgattg acaacgctcc
    10561 ggaagagcgc gagcgcggga tcaccattgc ggtttcgcac gtggagtacg aaacggcgaa
    10621 ccggcactac gcgcacatgg attgcccggg gcacgcggac tacatcaaga atatgatcac
    10681 gggtgccgcg cagatggatg gcgcgatcct ggtggtggcg gcgacggacg gaccgatgcc
    10741 gcagacgcgg gaacatattt tgctggcgcg gcaagttgga gttccggcgg ttgtggtgtt
    10801 tctgaacaag tgcgacatgg tggaagacgc ggagctgctg gagttggtgg agctggaagt
    10861 tcgcgagttg ctgaagagct accagtatcc gggcgatacg attgcggtgg tgaagggttc
    10921 ggcgctgcag gctttgaacg gtgacgcgaa gtgggaaaag acgattgaag aattgatgga
    10981 agcggtggac aagaacgtgc ctttgccggt gcgcgatatt gataagccgt tcgcgatgcc
    11041 gatcgaggat attttctcga tatcgggccg cggcacggta gtcaccggcc gaatcgagcg
    11101 cggcaaagtg aaggttggcg aagaaatcga gatcgtgggc ttccgcgcga cgcaaaagaa
    11161 agttgtgacg ggcgtggaga tgttcaagaa gttgctggat gaaggcctgg cgggcgacaa
    11221 cgtgggattg cttttgcgcg gcacggaaaa agaagacgtg gagcgcgggc aggtggtgtg
    11281 caagccggga agcatcacgc cgcacacgaa gttcaaggcc gaagcgtacg tattgacgaa
    11341 ggaagaaggc gggcggcaca cgccgttttt ctcgggttac cggccgcagt tttatttccg
    11401 gaccacggac gtgaccgggg atatgaagct gcccacgggc gtggaaatgg tgatgccggg
    11461 cgacaacgtg agctgcgaag tgacgttgat cacgccggtt gctttggaga agggcttgcg
    11521 gttcgcgatt cgcgaaggcg gccacaccgt cggcgccggt gcggtcactg aggttattga
    11581 ataattttgg ttttctaacc aaagatttta cgttctgaaa gggtcttagg tcatgcgcga
    11641 gatcattacg ttgcagtgcg gcgattgcaa gaaccgcaac tacacaacga cgaaaaacaa
    11701 gaagaaacac accgagcggt tggaaacgaa gaagttctgc aacacgtgcc gcaagcaaac
    11761 ggcacacaag gaagtgaagt agttttccgt ttttgcaagg gtcgttttag caggggcgag
    11821 gggcaggccg gatttgaatt taggggagtc gtccaacggc taggactccg gtctccaaaa
    11881 ccgggtatgg gggttcgagt ccctcctccc ctgcca
//
LOCUS       DASYVO010000044         8909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_15515, whole genome shotgun sequence.
ACCESSION   DASYVO010000044 DASYVO010000000
VERSION     DASYVO010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15515"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(434..1231)
                     /locus_tag="VGR03_01555"
     CDS             complement(434..1231)
                     /locus_tag="VGR03_01555"
                     /inference="COORDINATES: protein motif:HMM:NF037472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEV1992993.1"
                     /translation="MRTLALALAVLGTPFPCAFAQTTMSSMTSDFQDIQTAGGFLDVC
                     GLKESQAPKKSVDVVAKAPAGEVLDTFKIAMDATLADRKLCYGYLAGVIDGWQEGHEH
                     GVMAAHFPAGIPRDKSDPFRLELKSVSLNELQAMGAASKNDVPCLPDHITIGQTSDVV
                     IKYIRDYSLKNPFWRIMLTSRFVAPALRDAFPCPIRVGTVAVTSIPDGADINSDGVFV
                     GNSPATLKLSVGKHTVRVSMRGYKDWSRELTVLDGSEVKLAANLEKQ"
     gene            1573..2253
                     /locus_tag="VGR03_01560"
     CDS             1573..2253
                     /locus_tag="VGR03_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992994.1"
                     /translation="MDPEDSRLVDQAADIAASGRWAAVNERIEHLVANPGTDNDWWVQ
                     LFASLCSQVFSEYLFLKRAHEEKRDEPSLLAWRARNLLELSVWCLYCSKSRENARQLY
                     TDAGRDGLDLISAFRKWGAATAQDSDWLDLCTGAKQELSQRAASVGIESLDGSYKRVN
                     KAAKESGIGDHFTLSYGTLSKFVHPTAILILAPPDEAKTALLKDCFFSQGCLFFIGAF
                     VALEGQLL"
     gene            2535..2921
                     /locus_tag="VGR03_01565"
     CDS             2535..2921
                     /locus_tag="VGR03_01565"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1992995.1"
                     /translation="MKRVTGIGGIFFKAKDAPALQAWYKRHLGIDVQEWGGAAFTWTD
                     GEGKPVAGTTAWLIDSSKSDHFAPSEATFMVNYRVDDLHALVKALREEGCNVLEKIDE
                     SEYGKFAWVIDPEGNKVELWQPPAGQ"
     gene            complement(3580..3666)
                     /locus_tag="VGR03_01570"
     tRNA            complement(3580..3666)
                     /locus_tag="VGR03_01570"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3630..3632),aa:Leu,seq:tag)
     gene            complement(3720..5153)
                     /locus_tag="VGR03_01575"
     CDS             complement(3720..5153)
                     /locus_tag="VGR03_01575"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1992996.1"
                     /translation="MKTVKGTVHPEPADPLSLCIQLFLDGFFVRVETARGLLSATESA
                     ALLDLGLLTKDTKDESLLYSPIKLYPVDPVHVASDRWSNPDGSPFKSLPDILYPPVGP
                     NTKQFLEVLPDSPCEAFLDLCSGTGVAGLIAASQYARQAAAYDITERCAHFAEFSRRL
                     NALENFTTGTGDLYEPAAAQTFDRIVAHPPYVPVIEPKWIFYGGGEDGEQITRRIIAG
                     LPRYLRPGGALHCLAMVTERKGESLEHRVRQWLGGGEHGFDIAVVTRKTLDPGEFAVG
                     SVLKQGGKLSDTLKFKTLFRELKVTALTYAMIMVQRSASPRSTFTVRRQSGPRTSRAE
                     HEWLLGWETAAAESTGNDLVLQSRLRASAHCELLALNRIQDGDWAAVGYKLQVDYPFD
                     MEMRMEPWVGLLLPRCNGTLTGAEVFAALKKDGVLHAATPLGEFAALLRVLISGGFLL
                     IVSAAAGEVAAGEDGAAAGREGLTAMD"
     gene            complement(5359..6771)
                     /locus_tag="VGR03_01580"
     CDS             complement(5359..6771)
                     /locus_tag="VGR03_01580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1992997.1"
                     /translation="MAFSLPGQASFFALRLPEGQATGKPNWNSPGDSVQFAAQAESGS
                     TDLPEPAITAFTPPPQPTYRFAGLEWPSDVTSAERKTLLAGALGWMLDAMDVVLYSLV
                     LAELMRHFGMSKGTAGLLNSLTLAASAVGGLLFGFFADRIGRTRALMFSILAYSFTTA
                     ACGLSNTVLQLAVLRLFVGLGMGGEWSTGAALIAETWRAEHRGKALGLMQSSYAIGEM
                     IAVGVVFLVLPRFGWRMVFFVGILPAFIVFWILHSVPESEIWRKSAGQRRPSLARLWQ
                     KDLRRDTIVATAMNSFGMFGYWGLFTWIPGYLSLPVSQGGRGLALATSTSWLLLIGVG
                     KWFGYALFGFAADKFGRRGSYVTYLLVAAALVPVYANARNPLWLLLLGPVVAFFGIGF
                     FSGFSALAAELFPTEIRATAMGLSYNTGRGLSAAAPFLVGAIADRYGFQAGFYLLSAA
                     FFLAALLALALPETKGRQLV"
     gene            6770..7231
                     /locus_tag="VGR03_01585"
     CDS             6770..7231
                     /locus_tag="VGR03_01585"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEV1992998.1"
                     /translation="MAFCSKCGAQLTGNTAFCASCGQSQGTARAAASSESGLSENAAA
                     TLSYALGWITGLVFLLIDKRPYVRFHAAQSLVTFGGLHAIRICLGIVFGLGFVSGGML
                     GWSQFSFGLAIISLLGLLSLVLWIVCMIKASQGDRFKLPVVGDIAENIAGK"
     gene            7421..8377
                     /locus_tag="VGR03_01590"
     CDS             7421..8377
                     /locus_tag="VGR03_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1992999.1"
                     /translation="MVKVRLCFHDRCFDGAASSAVFLRFYRECITRDAEFALTGLTHR
                     AGNLFTPDLFDGDENAIVDFKYSSSPKLSWWFDHHQSAFLSPADAEHFRRDTSGRKFY
                     DPEYKSCTKFLSDVAQRKFGFDSAPLAELIHWGDMIDGAQYASAESAVSLREPATLLA
                     LVIEAAPEPGLVARIVPALAERPLKDMLKLPEVERQLPPLLERHEKSLEIFRKRAQAQ
                     GRVVYFDLSGDELEGYNKFIPYYLYPNCVYSVGVSTSPLRAKVSVGSNPWTPEPLQHN
                     LAALCERFGGGGHARVAAISLEPHDLEKARAVAQEIAAILRG"
     gene            8403..8750
                     /locus_tag="VGR03_01595"
     CDS             8403..8750
                     /locus_tag="VGR03_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEV1993000.1"
                     /translation="MSPTLNPQMVTTANDLTGFRVVKNLGLVRGIVVRSRSIIGTLGA
                     SLQTILGGNISLFTELCEKTREDAFELMMQHAAERGANAIVAMRYDANEVMQGVTEVL
                     AYGTAVQVERIAP"
     gene            8839..8909
                     /locus_tag="VGR03_01600"
     tRNA            8839..8909
                     /locus_tag="VGR03_01600"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8871..8873,aa:Lys,seq:ctt)
BASE COUNT         1883 a         2540 c         2742 g         1744 t
ORIGIN      
        1 attttgtaac tgcagaatcg aaagaacttg cgggcgtaga ttctgtaact gcagattctg
       61 aagcaattag agcgtgtggt cagtgtcccg tcccgaacct tcgggattcg ggagtggtca
      121 gggatcagtg aggggaagga agggtggagg atcgagattg cgggtgcgga catctatcct
      181 cctgaggcat agtatcaaag tgataatcgc ttgtcaaggg gaaaggagcg ctcgctacac
      241 ccgacttggt cccgcgtggt ccagtggagt tagagaatac cttcgaggag aagaccagga
      301 gtgacacaga gtgggaacaa gaaaagtccc catggttcgg cgccgctcac cacaagctac
      361 gtaaacccga cgtgttgggc acccaaattc atctcttcat ttagcgtctg tgccacccgc
      421 ccgtacgatt gcgctactgc ttctcaaggt ttgcagcgag tttgacctct gagccatcca
      481 gtacagttaa ttccctgctc caatccttat agccgcgcat agacacgcgc acagtatgtt
      541 ttccaacgct gagtttgagc gtggctgggc tgtttccgac aaagacccca tctgaattga
      601 tatcggctcc atcggggatc gaagtcacag ctaccgttcc cacccggatc gggcagggga
      661 acgcatcgcg tagtgcggga gcgacaaaac gggaagttag catgattcgc caaaacgggt
      721 ttttgagaga gtagtcgcgg atatacttga tgacgacgtc gctcgtttgg ccgatggtga
      781 tatggtcggg cagacaagga acatcgttct tagacgccgc acccatagct tgtagctcat
      841 tgagggacac ggactttaac tccaaacgga acggatcgct cttgtcgcgt ggaattccgg
      901 ccggaaagtg cgccgccata acgccgtgtt catggccctc ttgccagcca tcgatcactc
      961 cggccaaata tccataacag agttttcggt cggctagcgt cgcgtccatc gctatcttga
     1021 aggtgtccaa gacctctccg gcgggcgctt tcgccaccac gtccacgctt ttcttggggg
     1081 cctggctctc cttgagtccg caaacgtcca agaaaccgcc agcagtctgt atgtcctgga
     1141 agtccgaggt catggaggac atggttgttt gggcaaacgc gcaagggaac ggagtaccca
     1201 agacagcaag ggcaagggct aaggttctca tggagtggct cctgtggtcg ccattctcct
     1261 accccgagag ggacgaggca ataaattcct tcatcaaccg aagtgagcaa cgggcgttat
     1321 cattaacaat accaggctga tgcctaccga atgattcgga ggcgcgcgaa ggattgaggc
     1381 atcaagataa tgacaggaaa ccacactctc tgggcgacca gcatcaccgc tgacctcaag
     1441 aacggcagta agctcgaaat tgaccagtag atcgctacgc acgagtcatc gcgcacaacc
     1501 gggctccgcg accggcgcga cgatgacgtg agtctcgatg aagtagtaag gatctcgatt
     1561 tggaggcatc ttatggaccc tgaagattcc aggttggttg accaggcagc cgatatcgct
     1621 gcatcgggca ggtgggccgc cgtcaatgaa agaatcgagc atctggtcgc aaaccctggc
     1681 acagacaacg actggtgggt acagctattc gcctctttgt gttcccaagt tttttcggaa
     1741 tatttgttcc tgaagcgagc ccacgaggag aagcgcgatg agccttcact gctcgcatgg
     1801 cgcgcaagga atcttctaga gctctcagtt tggtgtttgt actgctcgaa gagtagagaa
     1861 aacgcacgcc aactctacac ggacgccggc cgcgacggac ttgatcttat tagtgcgttc
     1921 agaaaatggg gggctgcgac ggcccaagat tccgactggc tcgatctgtg tacgggcgcg
     1981 aaacaagagc tttcccaacg ggctgcttcg gtcgggattg agtctctaga tgggtcttac
     2041 aagcgagtca ataaggcggc taaagagtct ggaatcggtg atcatttcac ccttagttac
     2101 ggaacgttgt ccaagttcgt gcacccaacc gccattctaa ttctggcccc tcccgatgaa
     2161 gcaaagaccg ccctactaaa ggattgtttc ttcagccagg gttgcctttt cttcattgga
     2221 gctttcgtgg cccttgaagg ccaattgctt taagccgctc aactattagg ggtcactgct
     2281 cagcaaaacg cggtgcggtg ggctgcgcat tggaattcgt cttagtgctt tcctgagcgc
     2341 acctcacgac gagagtatat caccactgta cttacttgca gagtgatgag acgaggtgcg
     2401 gcacccaaaa cctcagcccg tctcaaagcc tccgccaccc gccccgctca tcgtcggctg
     2461 tgatataaac tctctcctgc ccactggtac aaaatctcgg gctggtcatc accatactcg
     2521 atctcggagg ttctatgaaa cgagttactg gcattggcgg cattttcttc aaagccaaag
     2581 acgctccagc actacaagcc tggtacaaac gacatctcgg gatcgatgtc caagagtggg
     2641 gtggcgcagc gttcacgtgg accgacggtg aaggtaagcc ggttgccgga acaacggcct
     2701 ggttgattga ttcgtcgaaa agcgaccact ttgcacccag cgaagccacc ttcatggtca
     2761 attaccgcgt ggacgatctc cacgcgctgg tcaaagcctt gcgcgaagaa ggttgcaacg
     2821 tacttgagaa gattgacgaa tccgagtatg gaaagtttgc ttgggtcatc gatccggaag
     2881 gaaacaaggt ggaactatgg caaccgcctg ccggccaatg acaaaggctg tcgggcgcag
     2941 tgcaaatttg cagacttgta gtgctccccc ctaatttaga cagtcagggc ctcgtttttt
     3001 agtacagctg tgaaagccaa gtaggcctcg tgcggagtgc gattgtggac cccgcgatga
     3061 gggcgggtgg cggattggaa ttcgtctgaa ttttctcttg aggtcaccgc acgaggagag
     3121 tgtaccaccg atgtaattac tcccagagtg atgaggcgag agaaagggtc cctcggctca
     3181 ctgcgttcgc tcgggacagc ctttcctagt cggaaaggct gatccacacg cgcgtaccca
     3241 acccagcaaa tggagctccg ccctcgacca gctcttgctt cttggctctg ttccagctct
     3301 tgatctgctt ctctcgctta gctgcaccac tgcgggatcg gtgggcttca taccaaacca
     3361 gggccactgg tttggcgtcc ttcgtatagc ctgaacctct acccgaagca tgatcgcgga
     3421 ctcgtcgaga cagattcgaa gcgatgccac agtaatacga gtgatcggca cagagcagca
     3481 ggtagcatcg ccagccgggc gcaaattcgg gaagatttag gataaggagt ccctcggctc
     3541 actgcgttcg ctcgggacag ccttcctggt cggaaaggct ggtgcggaag gagggagtcg
     3601 aacccccacg gcctttcggc cacaggatcc taagtcctgc gcgtctgcca gttccgccac
     3661 ttccgcggcg taaggcgaag gtagcacggc ggagcgatgg ggctcaagcg accttcgtgt
     3721 caatccattg ccgtgagccc ttccctgcct gccgcagccc cgtcctcgcc tgcggcgacc
     3781 tctcccgcgg cggccgagac aatcagaaga aacccgccgg aaatcaatac tctcagcaac
     3841 gcagcaaact cgccgagcgg cgtggcggcg tgaaggacac catctttttt cagcgcggcg
     3901 aagacttccg cgccggtcag cgtgccgttg cagcgaggca gcagcagtcc gacccagggc
     3961 tccatgcgca tctccatatc gaagggataa tcgacctgca gtttatatcc caccgccgcc
     4021 cagtcgccgt cctggatgcg gttcaacgcc agcaattcgc aatgcgcaga tgctctgagc
     4081 cgcgactgca agaccaaatc gttgccggtg gactccgcgg cggcggtttc ccaccccagc
     4141 agccattcgt gctcggcgcg cgaagttcgt ggaccggact ggcggcgcac ggtaaacgtg
     4201 ctgcgcggac tggcgctgcg ctgaaccatg atcatggcgt aggtcagcgc ggtgaccttc
     4261 agctcgcgga agagcgtctt gaatttcaaa gtgtcgctaa gttttccacc ctgtttgagg
     4321 accgagccca cggcgaactc gccggggtcg agggttttgc gcgtgaccac ggcgatatcg
     4381 aatccgtgct caccgccgcc gagccactgg cgcacgcgat gctcgagcga ttctcccttg
     4441 cgttcggtga ccatcgccag gcagtgtaac gcgccgcccg gccgcagata gcgcggcagg
     4501 ccggcgatga tgcggcgcgt gatctgctcg ccgtcttccc cgccgccgta aaaaatccat
     4561 ttgggttcga tcaccggcac gtagggaggg tgagcgacga tgcggtcaaa cgtctgcgct
     4621 gcggcgggct cgtaaagatc gcccgtgccg gtggtgaaat tctcgagcgc gttgagccgc
     4681 cggctgaatt ccgcaaaatg cgcgcagcgc tcggtgatgt cgtaggccgc ggcctgccgc
     4741 gcatattgcg aggccgcgat caaccccgcg actcccgtgc cggagcacag gtccaggaaa
     4801 gcctcgcacg gagagtcggg aagcacctcg aggaactgtt ttgtgttcgg gccaaccggt
     4861 ggatagagga tgtccggaag actcttgaaa ggagagccgt ccggattgct ccagcggtcc
     4921 gaagcgacgt ggacgggatc gaccggatag agcttgatgg gcgagtacag gagcgattcg
     4981 tctttcgtgt ccttggtcag cagaccaaga tcgagaagcg cggcgctctc ggtggcggaa
     5041 agcagaccgc gcgcggtctc gacgcggacg aagaagccgt cgaggaacag ttggatgcaa
     5101 aggctgagcg ggtccgcagg ttcgggatgg accgttccct tcaccgtctt catttccgtt
     5161 tcagcgaggt cgtgaatatc aacgaatggt acgacgccgg acgcagtaaa atttgcgcgg
     5221 cgcagcaatt cgcgcacgca ggagaactgg tcggatgtgc ctaaccgaat tggtaacgga
     5281 gaaagattca cgggaacaaa gattacacgc gggctggctt gaatgccaca ggcgcggaaa
     5341 attgacgcgc gctcagatct atacgagttg cctacccttg gtctccggaa gggcgagagc
     5401 aagcagcgcg gcgaggaaaa aggcggcgga gaggaggtag aagccggcct ggaacccgta
     5461 gcggtcggcg atggcaccga ccagaaaggg cgcggcggcc gaaaggccgc ggccggtgtt
     5521 gtagctcaaa cccatggccg tggcgcgaat ctcggtgggg aacagttccg cggcgagcgc
     5581 gctgaaaccg gaaaagaagc cgatgccgaa aaaagccacg acggggccaa gcaacaaaag
     5641 ccagagcggg ttcctggcgt tggcgtaaac cggcacaagc gctgcggcga cgagcaggta
     5701 ggtgacgtaa cttccgcggc ggccaaattt atccgcggcg aatccaaaca gcgcgtagcc
     5761 gaaccacttt cctactccga tcaggagaag ccaggaggtc gaggtggcaa gagcaagtcc
     5821 cctgccgccc tgcgaaacgg gcagcgagag atagcccgga atccaggtga acagccccca
     5881 gtaaccgaac atgccgaagg aattcatggc tgtggccacg atggtgtcgc gccgcagatc
     5941 cttctgccac agcctggcca gcgacggccg gcgctggccc gcgctttttc gccagatttc
     6001 ggactccggg acggaatgaa ggatccagaa aacgatgaac gcgggaagaa tgcccacgaa
     6061 aaacaccatg cgccagccga aacgcggcag gacaagaaac accacgccga cggcgatcat
     6121 ttcgccgatg gcgtagctcg attgcatcaa gccgagcgct ttgccgcgat gctcggcgcg
     6181 ccaggtctcg gcgataagtg cggcgcccgt gctccattcg ccgcccatgc cgaggcccac
     6241 gaacaggcga agaaccgcca actggaggac ggtattcgag agaccgcagg cggcggtggt
     6301 gaatgaatag gcgaggatgg aaaacatcag ggcgcgtgtg cggccgatgc ggtcggcaaa
     6361 aaagccgaag agcaggccgc ccacagcgga agcggcgagc gtcagcgaat tgagaagtcc
     6421 cgcggtgccc ttgctcatgc cgaagtggcg catcaattcg gcgaggacga gcgagtagag
     6481 caccacgtcc atggcgtcga gcatccatcc gagtgcgccg gccaggagag ttttgcgctc
     6541 ggccgaagtg acgtcgctgg gccattcgag tcccgcaaac ctgtacgtcg gttgcggcgg
     6601 cggggtgaag gcggtgatgg cgggttcggg caggtctgtg ctccccgatt cagcttgcgc
     6661 cgcgaattgt actgaatctc ccggcgagtt ccaattcggc ttgccggtgg cctggccctc
     6721 tggtagcctc agcgcaaaga aggaagcctg acccgggagg gaaaacgcca tggccttttg
     6781 cagcaagtgc ggtgctcagc ttaccggcaa cacggcgttc tgcgccagtt gcggccagtc
     6841 gcaggggacg gcccgcgctg cggcgagcag tgaatccgga ctctccgaga atgcggcggc
     6901 cacgctgagt tatgcgctgg ggtggatcac tggtctcgtt tttctgctga tcgacaagcg
     6961 tccttacgtc cgcttccatg ccgcgcagtc gctggtgacg tttggagggc tgcacgcgat
     7021 ccgcatctgc ctgggaattg tcttcggctt agggttcgtc tcgggcggca tgctgggctg
     7081 gtcgcaattc tcgttcggcc tggccatcat cagcctgctg ggcctgctca gcttggtcct
     7141 gtggattgtg tgcatgatca aagcgtcgca gggtgaccgt ttcaagctgc ccgtggtcgg
     7201 ggacatcgcc gagaatatcg cgggaaaata agcgagaggg tgcgcgaccc aactccatag
     7261 aagacctgct catgccggtc cgcgcgggaa aaagaatttc ccgggcgtga ggattcctgc
     7321 ggcgggcttt ccgcttgaca atccttcgat gagacgccat ctttatggac tttgctgaat
     7381 tgggaatttt catccatgcg cgcgggcgcg tcctagccag atggtcaagg ttcgcctatg
     7441 tttccacgat cggtgctttg acggcgcggc ctcttcggct gttttcctgc gcttttaccg
     7501 cgagtgcatc acgcgagacg ccgagttcgc gctgaccggg cttacccacc gcgcgggcaa
     7561 tctgttcacg ccggacctgt tcgacgggga tgaaaacgcc atcgtcgact tcaaatactc
     7621 gagttcgcca aagctgagct ggtggtttga ccaccaccag agcgcctttc tctctcctgc
     7681 cgacgcggaa catttcaggc gcgacacttc cggccggaag ttttacgatc cggagtacaa
     7741 atcctgcacc aagttccttt ccgacgtggc gcagcggaaa ttcggctttg attccgcccc
     7801 gctggcggag ctgattcact ggggcgatat gatcgacgga gcgcaatacg ccagtgcgga
     7861 gtcggcggtg agtctgcggg agcctgccac gctcctggcg ctggtgattg aggccgcgcc
     7921 ggagccgggt ctggtagcgc gaatcgttcc ggcgctcgcg gagcgcccgc tcaaggacat
     7981 gctgaagctg cccgaagttg agcgccaact gccgccgctc ctcgagcggc acgaaaaatc
     8041 actggagata ttccggaagc gggcgcaggc ccagggccgc gtggtctatt tcgacctgag
     8101 cggcgacgag ctggaaggct acaacaaatt cattccctat tatctgtacc cgaattgcgt
     8161 ctattccgtg ggcgtgagca cttcgccgct gcgcgcgaaa gtatcggtgg gctcgaaccc
     8221 ctggacgcct gagccgctcc agcacaacct cgccgcattg tgcgaacgct tcggcggggg
     8281 cggacacgcg cgcgtcgcgg cgatttcgct cgagccgcac gatctcgaga aggcgcgtgc
     8341 ggttgcgcag gagatcgccg ccatcctgcg gggctagccg ggcaacggaa aggagttccg
     8401 aaatgtcgcc gacgctgaat ccccagatgg tgaccacagc caacgacttg acgggttttc
     8461 gcgtggtgaa gaacctcggg ctggtgcgag gcatcgtggt gcgctcgcgc agcatcatcg
     8521 gcaccctggg cgcttcgctg cagaccatcc tgggaggaaa tatttcgctg ttcaccgagt
     8581 tgtgcgagaa gactcgcgag gacgcgttcg agctgatgat gcagcacgcc gccgagcgcg
     8641 gcgcaaacgc catcgtggcc atgcgctatg acgccaatga ggtgatgcag ggcgtcaccg
     8701 aagtgctggc ctatggcacc gcggtgcagg tggagcgcat cgcgccgtag cgagcgcttg
     8761 cggcggcggc aattcaggcc tcacgtccgc gtcttgctca ccacgccgaa gtctcgttat
     8821 acttctaacg ttgtctgtgg gccgctagct caatggtaga gcaattgact cttaatcaat
     8881 aggttccagg ttcgagtcct gggcggctc
//
LOCUS       DASYVO010000045         7284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_269655, whole genome shotgun sequence.
ACCESSION   DASYVO010000045 DASYVO010000000
VERSION     DASYVO010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269655"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VGR03_01605"
     CDS             complement(<1..188)
                     /locus_tag="VGR03_01605"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HEV1993001.1"
                     /translation="MKIRGLMMDPVTNMPIVVLKDVQGQAILPIWVGMYEANAIALEI
                     EKVQTPRPMTHDLLKNVLL"
     gene            complement(216..1610)
                     /gene="miaB"
                     /locus_tag="VGR03_01610"
     CDS             complement(216..1610)
                     /gene="miaB"
                     /locus_tag="VGR03_01610"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEV1993002.1"
                     /translation="MSKPFDIELERLTGQPSYSEVVRGAAAGPRGTFFLETFGCQMND
                     HDSEKVAGLLLSRGYQQVETPEAASMILYNTCSIREKAAQKVFSRLGEYREKQNEGKV
                     IGVLGCVAQQEGENIFKRAPWVSLVCGSASYSKLPQMLAQLEAEHQRVTGLDTDTDET
                     FETEVTRRDNPWRAYLTIIEGCDKACSYCVVPFTRGPERSRASDSILREVRELAELGY
                     SEVQLLGQTVNSYADPTARRMRFSELLLAVADVQGIRRVRFTTSHPSDFTPDIVEAIE
                     RQPKICDHVHLPVQSGSTRMLRAMQRTYSREEYLEKIAIIRGADRPIAITTDIIVGFP
                     GETESDFEETISLLQEVQYTGLFAFKYSPRPNTPSLSMNDAIPEEEKGRRLAVVQEKQ
                     REIQAAGNAELAGYTFEVLVSGKSRRENQWSGYTTSHRMISFASQERALLGTYIKVRV
                     TGAGPNSLVGEHAV"
     gene            1715..2644
                     /locus_tag="VGR03_01615"
     CDS             1715..2644
                     /locus_tag="VGR03_01615"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HEV1993003.1"
                     /translation="MKLARIICILSFFVLLLMAVPLLAQDLPKDYQEVLTFLGRAGDF
                     KAKVLKVNIPRSDLHVTIAGQSTPTPFGFGGWVAMTKGDGGSEVLMGDLVLTQEEVNP
                     VLSALLDHGIEVTALHNHFFSEEPRLFYMHVHGHGEALNLARAIKPALDLIGHISSAP
                     ADAKPTAAPAASATTFPFDTAALNKIAGVDGEKLGSVYKFTLGRDDLHLTEMGATINA
                     RMGLNSWAAFAGNDDDAQVAGDIAMRASEVNAVLKALRSHGLNIVAIHHHMLDTQPNV
                     IFLHYWGRGPAAKLAADFRAALDQLGTGKSLSK"
     gene            2699..3843
                     /gene="chrA"
                     /locus_tag="VGR03_01620"
                     /pseudo
     CDS             2699..3843
                     /gene="chrA"
                     /locus_tag="VGR03_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
     gene            3884..4891
                     /locus_tag="VGR03_01625"
     CDS             3884..4891
                     /locus_tag="VGR03_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEV1993004.1"
                     /translation="MRKFFPASVSACLLAVAMCGALLAGVSPLRKIAQVELPGPKGKR
                     FDYVTVDPDDHFVIVAHMHGDQTYFVDTRTNQLAATITDTPGVEYVPDQRKVYTSNSG
                     DNTIGVIDIAQRKVIMKLKTEAKPDGSTYAAPFHKLYVSDERGKAVAIVDVRSDTIMK
                     TLHFDSETGMPQYDPVAKLAYVNLQDTERFAVIDPQDDRVLSQFPVAGCRGNHGMALA
                     PEQRRAFLVCEGNNLLAVFDLEKHTVVQTLPLPEGGDVVKADPGRGRIFVACYSGAIT
                     AIQALGSGQYKKIADAPVEKRVHSLAINPETGYLYVPEEQEKGTPASRLIVYALPGEV
                     R"
     gene            5051..5731
                     /locus_tag="VGR03_01630"
     CDS             5051..5731
                     /locus_tag="VGR03_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEV1993005.1"
                     /translation="MFTTILVTDIGNILTQTGWVARAVLLLLLLFSIVSWAMILQKIG
                     LFGRIRRESDQFLRIFRATRGVANPQALATAGSPFSSVYAAGYRELQSQVGSAPGNPH
                     PPKLKSLSAVTVSMQLASSDEVRRVEKGMSWLATTGSVTPFIGLFGTVWGIIDAFSGL
                     GSAGAASLRAVAPGIAEALVTTAAGLFAAIPAVIFYNQFLQNIRDLAQRLDTFALEFT
                     AQVEKTFD"
     gene            5764..6198
                     /locus_tag="VGR03_01635"
     CDS             5764..6198
                     /locus_tag="VGR03_01635"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEV1993006.1"
                     /translation="MGSRKMPQLSKSTQTSLSEINMVPFVDVVLVLLIIFMITAPILQ
                     SGIEVDVPKTRTVKEINQERLVVTVDKAQRIYLGNEPINIHQLGSRIRAQLKNTQEGV
                     FLRCDETVPFGSFAAVVDALRQSGINNISIVTEPITTRARTQ"
     gene            6198..7028
                     /locus_tag="VGR03_01640"
     CDS             6198..7028
                     /locus_tag="VGR03_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEV1993007.1"
                     /translation="MMTHAVANSMDPIGGTRYEDQSLRQFLIYSIIMHASLAVVIVVS
                     AYIQYRGTAWGGIGGDLGGTKVSLVRAAGIPLPKESVVTESKAVDPTKGLHKEEPPKL
                     PEPKTDATKIPKFTKEKPLPPSRQSRTLENKTPTPDNAVPYGKGGNPDLPTGFSQTPG
                     EGSSGVTVQGQGGADFASRYGWYIEAVKNRIYGNWQQWTIDAAARASRTMHCAITFTI
                     NRDGSLKDAHISESSGNTSYDNSALRAVLSSTPVTRLPPDYSGAYVVATLDFNPPSIR
                     "
     gene            7077..>7284
                     /locus_tag="VGR03_01645"
     CDS             7077..>7284
                     /locus_tag="VGR03_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993008.1"
                     /translation="MQAKLRGLSSFLVLVILAFCGRSVAAQDWFRTAIGMDANKPRLA
                     IADFAARSDSAKPHASLFTQVVRDD"
BASE COUNT         1443 a         2274 c         1936 g         1631 t
ORIGIN      
        1 agcaaaacgt tcttcagcag gtcatgggtc attggccggg gagtctgcac tttctcgatt
       61 tccagggcga tggcatttgc ttcgtacatg cccacccaga tcggcaggat cgcctgcccc
      121 tgcacgtcct tgagcaccac gatgggcatg ttcgtgacgg gatccatcat caggccacgg
      181 atcttcatct ccacttccat cgaggcctcc ctgagctaaa cggcgtgttc ccccaccaga
      241 ctgtttggac ccgctcccgt aacgcgaacc ttgatgtagg ttcccagaag cgctctctct
      301 tgtgaagcga aacttatcat gcggtgcgat gtggtatacc cggaccactg gttctcgcgg
      361 cgcgattttc cgctgaccag aacttcaaac gtatagccgg ccagctccgc attcccggcc
      421 gcctgaatct cgcgctgctt ctcctgcacc acggcaagcc gccggccctt ctcctcttcg
      481 ggaatcgcat cgttcatgga gagagacgga gtattcggcc tcggagaata cttgaacgcg
      541 aacaagccgg tgtactgcac ttcctggagc aggctgatgg tttcctcgaa atcggattcc
      601 gtttcgcccg gaaaacccac gataatatcc gtagtgatag cgatcggcct gtcagctccg
      661 cgaattatgg cgatcttttc cagatactct tcgcgcgaat aggtccgctg catcgcgcgc
      721 agcattctcg tcgaaccgga ctgcaccggc aagtgaacgt gatcgcagat cttcggctgg
      781 cgctcgatgg cttccacaat atctggcgtg aaatcactgg gatgtgaagt agtgaaccgc
      841 acgcggcgaa ttccctgcac atcggccacc gccagcagca attccgaaaa acgcatcctt
      901 cgcgctgtcg ggtccgcgta agagttcacc gtttgcccaa ggagttgaac ttccgaatag
      961 ccgagttccg caagctctcg cacttctctg agaatggaat cgctcgcgcg gctgcgttcg
     1021 ggaccgcgcg tgaaaggtac cacgcagtac gagcacgcct tgtcgcagcc ttcgatgatc
     1081 gtcaggtagg cgcgccaggg attgtcgcgg cgcgtcacct ccgtttcaaa tgtttcgtcc
     1141 gtgtccgtat ccagcccggt cacgcgttga tgttcagctt ccaattgcgc gagcatctgc
     1201 ggcagcttgc tgtaacttgc cgaaccgcac accaggctca cccacggcgc gcgcttaaaa
     1261 atattttcgc cttcctgttg tgccacacag cccagcactc cgatgacctt gccctcgttt
     1321 tgcttctccc ggtattcccc caggcgcgag aaaactttct gcgccgcttt ctcgcgaatg
     1381 ctgcaagtgt tgtaaaggat catggaagcg gcttccggcg tctccacctg ctgatagccg
     1441 cgcgacagca gcaggcccgc gaccttttcc gagtcgtggt cattcatctg gcagccaaac
     1501 gtctccagga aaaatgttcc gcgcggcccc gcggctgcgc cacggactac ctcggaatac
     1561 gagggctggc cggtcagtct ttccaattcg atgtcgaacg gtttgctcat gcccaaatcg
     1621 cagtttatca cacctccttt actctttcgg ggcaacgcat tgtcgctcga ttctggccgt
     1681 cttcggtgta aagtggaggc tgaggagcgt cgcaatgaaa cttgcccgca ttatttgcat
     1741 tttgagcttc ttcgtcctgc tgctgatggc ggttcccctc ctcgcccagg acttgcccaa
     1801 ggactatcag gaagtcctca cgttcctcgg ccgcgcgggc gacttcaaag ccaaagtatt
     1861 gaaggtcaat atccctcgca gcgacctgca cgtaaccatt gccgggcaat ctaccccgac
     1921 gcccttcggc ttcggcggct gggtggccat gacaaaaggc gatggcggca gcgaagtcct
     1981 gatgggtgac ctcgtcctga cgcaggaaga agtcaatccg gtcctttccg ccttactcga
     2041 ccatggcatt gaggttaccg cgctgcacaa ccatttcttt tccgaggagc cgcgcctctt
     2101 ctatatgcat gtccatgggc acggcgaagc gctgaatctt gcccgcgcca tcaagcccgc
     2161 gctcgatctc atcggacaca tttcatccgc ccccgcggac gcaaaaccca ccgccgcacc
     2221 cgccgcttcg gcaacaacct tccccttcga taccgctgcg ctcaataaaa ttgccggcgt
     2281 cgacggcgag aaactcggct ccgtctacaa attcaccctg ggccgcgacg acctgcacct
     2341 taccgaaatg ggtgccacga ttaacgcccg catgggattg aactcctggg cggccttcgc
     2401 gggaaatgat gacgatgctc aagtcgccgg cgacatcgcc atgcgcgcga gcgaagtcaa
     2461 cgccgttttg aaggccttgc ggtcgcacgg cctgaatatc gttgcgatcc accatcacat
     2521 gctggatacc cagccaaacg tgatttttct ccactactgg ggccgcggcc cggcggccaa
     2581 gcttgctgcg gattttcgtg ccgctctgga ccaactcggc actggcaagt ctctgagcaa
     2641 gtaagttagc gggaacttga gccaaccatc cgagtttgag atagaaactc caagcggagt
     2701 gagcccgaag ggcacgctgc cgcaattcgt ctcctatttt ctgcgtttgg gtacgtttgg
     2761 cttcggcggg cctatcgcgc tcgctgcccg gatgcaaaaa gaccttgtcg aagaactacg
     2821 ttggatctct caagaggact acaccgaagg gttggcgttt tctcaactct cgcccggtcc
     2881 cctggcagcg caactcgcga tgtacctcgg ctgggtccgc tacggaaatt ggggcgcaac
     2941 cctgacgggt gcattcttta ttttgccgtc attcctcatg gttttgggga ttgccgccct
     3001 ctatctccac tatggaagct tgtcatcgat tcaatccatc ttttatggag taggcgcggc
     3061 cgtcgtagct atccttgctc gtagcgccta caaactgact cgaacgacct tgaaaaccga
     3121 ttacctgctt tgggtcttgt tcacgataag tgccgtcgcc acggcctgga ccaagtcaga
     3181 aatcatctgg ctgtttgtgc tttgcggtgt cgtcgccatg atcgtcaaaa cgcggttgcg
     3241 agccgctgca gcctccgtgc ttcccgccgg cctgacgtgg ctcaccacag gaatccatgg
     3301 ccctgccagt ctgaccaccg gttttggact gtttactttt ttcctcaaag caggagcctt
     3361 tgtcttcggc agcggcctgg ccatcgtgcc gttcctctac ggcggacttg tgcttcagtc
     3421 gcactggttg acggaacgtc aattcttgga tgccgtggct gtcgccatga ttactccggg
     3481 gcccttcgtc accaccgctg ggtttatcgg gtacctggtt gcgggaccgt gggcgcagcg
     3541 ctcgctgcgg tagcggtttt tgctccgcct tatttcattg tgttggtcgc cgccccattt
     3601 tatcgccgtt tcaccaagaa tctctacgtg aatacattcg tgaaaggggt gactgccgcc
     3661 gccgtcgggg ctatcaccgg agcagtcttc attttgggct cgcgcacctt gattgatcgg
     3721 aattctcttc tcatcgcggg cgcatcggtg ggtattcttt cgctaacaaa aaaggttccg
     3781 gagccagttc tcattctgat ggccggactg gtgggacttt tcctgctgcg tggcgtacat
     3841 tagctgctgc gagcaccatc atgaaatgag aggaggtcat cttatgcgca agttctttcc
     3901 cgcgagtgtc tcagcatgtt tgttggcggt agcaatgtgc ggcgcgctgc tcgcgggagt
     3961 gtcacctttg cgaaaaatcg ctcaggtcga gttgccaggc ccaaagggca aacgcttcga
     4021 ctacgtgacg gtcgacccag acgatcattt cgtcatcgtg gcgcacatgc atggcgatca
     4081 gacgtacttt gtcgatacgc gcacgaatca gctcgccgcg acgatcaccg acacgcccgg
     4141 cgtcgaatac gtccccgatc agaggaaggt ctacacttcc aactccggcg acaacaccat
     4201 cggcgtgatc gacatcgccc aacgaaaagt catcatgaag ctcaagaccg aagccaagcc
     4261 cgatggcagc acctacgccg ccccgtttca caaactctac gtttcggacg aacgcggcaa
     4321 ggccgtagcg attgtggacg tgcgcagcga caccatcatg aagaccctcc atttcgacag
     4381 cgagaccggg atgccgcagt acgatcccgt cgcaaaactt gcctacgtca acttgcagga
     4441 caccgagcgc ttcgccgtca tcgatccgca ggacgaccgc gtcctttcgc aatttcccgt
     4501 tgccggttgc cgcggaaatc acgggatggc tttggcgccg gagcagcgac gcgctttcct
     4561 cgtctgcgaa ggaaacaatc tcctggctgt cttcgatctg gagaaacaca ccgtggttca
     4621 gactcttcct ctaccggaag gaggcgacgt cgtgaaggcc gacccaggtc gggggcgcat
     4681 ctttgtcgcc tgctacagcg gcgccattac ggcaattcaa gccctcggtt cgggccaata
     4741 caagaagatc gccgatgctc ccgtggagaa gagagttcat agccttgcta tcaatccgga
     4801 aaccggctat ctctacgtgc cggaagaaca ggaaaagggc actcccgctt ccagactaat
     4861 tgtctacgcc ctgcccggcg aagtccgcta agttcgccgc ccgttgcccg ataagcagcg
     4921 aaaccagaaa cttacgcatc ctactggttg cgtgctctgg atgcgtgtgc tacgtttctt
     4981 acgcccagaa aaatgttgac cgcgtcgcgg tcacaaatca tttgctctca ctgaggggta
     5041 catcattttt ttgttcacca cgattcttgt aactgatatc ggaaatatcc ttacgcaaac
     5101 cggctgggtc gcccgcgccg ttcttcttct cctgctttta ttctcgatcg tttcttgggc
     5161 catgattctc cagaaaattg gtctttttgg ccgcatccgc cgcgagagcg accagtttct
     5221 gcgcattttt cgcgccacgc gaggcgtggc gaatccgcaa gccctggcaa ccgccggctc
     5281 cccgttttct tcggtctatg cggcgggcta ccgcgagcta cagagccagg ttggcagcgc
     5341 cccgggcaat ccgcatccac ccaagctgaa gagcttgtca gccgtcaccg tcagcatgca
     5401 gctcgcctcc tcggatgagg tgcgccgcgt cgaaaaaggc atgtcctggc tggccaccac
     5461 cggttccgtg acgcccttca tcggtctgtt cggaaccgtg tggggcatca tcgacgcatt
     5521 ctccggcctg ggcagtgccg gtgccgccag cttgcgcgcc gtcgcgccgg gcattgctga
     5581 agcgctcgtc accaccgccg ccggtctttt cgccgccatc ccagcggtaa tcttctacaa
     5641 ccagtttttg cagaatattc gcgatctcgc gcagaggctg gacacctttg ctctggaatt
     5701 cacggcgcag gtcgagaaaa cgtttgacta gaatttcgcg gtcgtcccta ttcggccgca
     5761 tccatgggtt cacgaaagat gccgcaactc tccaaatcga cgcagacttc gctctccgag
     5821 atcaacatgg tccctttcgt ggacgtggtt ctcgtgctgc tcatcatctt catgatcacc
     5881 gccccgattc tccagtcggg catcgaagtc gacgtcccca agacgcgaac ggtcaaggaa
     5941 atcaaccagg agcgtttggt cgtcaccgtc gacaaggccc agcgcatcta ccttggcaat
     6001 gagcccatta acattcacca gctcggctcc agaatccgcg cccagttgaa aaacacccag
     6061 gagggcgtct tcctccgctg cgatgagacc gttccgttcg gcagttttgc cgccgtcgtc
     6121 gatgcgcttc gccagtccgg catcaacaat atcagcatcg tcaccgagcc catcaccacc
     6181 cgcgcgcgta cccaataatg atgacgcatg ccgtcgccaa ttcgatggac cccatcgggg
     6241 gcacacgata cgaggaccag agcctccgcc agttcctcat ctactcgatc attatgcatg
     6301 ccagcctggc cgtcgttatt gtcgtctccg catatattca ataccgcgga accgcctggg
     6361 gcggcattgg gggcgacctc ggcggcacca aggtgagcct ggttagagcc gctggaatcc
     6421 ccctgcccaa agaatcggtc gtcaccgaga gcaaggcggt cgatccaacg aagggccttc
     6481 acaaagagga gcccccgaaa ctgcccgagc caaaaaccga cgcaacgaaa attccaaaat
     6541 tcacaaaaga aaaaccgctg cccccttcgc gccaatcgcg aaccctcgaa aacaagacgc
     6601 ctactccgga taatgcggtt ccttacggca aaggtggcaa tcccgatctt ccaacgggct
     6661 tttctcagac ccccggtgaa ggctctagcg gcgttaccgt tcagggacag ggcggagcag
     6721 atttcgcttc ccgctatggc tggtacatcg aagccgtgaa gaacagaatt tatggcaact
     6781 ggcagcagtg gacgatcgat gcagccgcgc gcgcctcgcg taccatgcac tgcgcgatca
     6841 catttaccat caatcgcgac ggctcactca aggacgcgca tatttccgaa tccagcggga
     6901 acacttccta tgacaattcg gcgttgcggg ccgtgctaag ctcgactccg gtgaccagac
     6961 tgccgcccga ctattcaggc gcttatgtcg tcgccacttt ggatttcaat cccccgtcca
     7021 ttcgataagg acgtgagttc tggttcctgg aatttgccat gttgtcggag gtgagggtgc
     7081 aagcaaagct cagaggcctt agttcgttct tggtcttggt gattctcgct ttctgcgggc
     7141 ggtccgtcgc ggctcaagac tggtttcgca ccgcaatcgg catggatgcc aacaagcccc
     7201 gcctggcgat tgcagacttc gcggcgcggt ctgattccgc aaaaccgcac gcttcccttt
     7261 tcacacaggt tgtgcgcgat gatc
//
LOCUS       DASYVO010000046        15933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_71233, whole genome shotgun sequence.
ACCESSION   DASYVO010000046 DASYVO010000000
VERSION     DASYVO010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 15933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15933
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71233"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VGR03_01650"
     CDS             <1..991
                     /locus_tag="VGR03_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1993009.1"
                     /translation="GASGAGKSTLLDCIAGLARPNEGRIVAGGDVLFDSARGIHVPAP
                     KRRIAYVFQTLALFPHLSAEENVVYGLRDLREEERQERVGAILKAFRVEKLRKQKPGE
                     ISGGERQRIALARSLVTEPRVLLLDEPLTGLDAELKTAIVDDLRAWNTAKGIPIMYVT
                     HSREEVDALGERVIALDNGRVASTGAPMEVLDAPRRKRLAQAAGFENLLSSTVLDLRE
                     ADGVMRVRLGESACEIEVPLGYAAAGNRVQVAVRAGDILLATERPHGLSARNVMEGRI
                     VSLEQRGPMVIARVDCGVTFVVHITPGAVRSLELTAGRSVWLVLKTHSCHLVD"
     gene            complement(1019..1375)
                     /locus_tag="VGR03_01655"
     CDS             complement(1019..1375)
                     /locus_tag="VGR03_01655"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEV1993010.1"
                     /translation="MKLRLSTLALLFVLVTGAAFAHGNKVHVRGTVEKISVDSLFVKT
                     ADGKSVEVKFAASTVFVSRSSNEDKPAKAGDLAAGDLVVIHATPKDNTLEADEIKFSV
                     PVAAKPAKPAPAKPKS"
     gene            complement(1417..2037)
                     /locus_tag="VGR03_01660"
     CDS             complement(1417..2037)
                     /locus_tag="VGR03_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV1993011.1"
                     /translation="MKRRTLVIIVAVALLGLVVAVAASFLHDGLSSRATPNKFEIMVA
                     RNARHLAIPSNARLAQNPFLASAEDLRDARLHFADHCAVCHANDGGGQTMIGKGLYPK
                     PPDLRLPETQNLTDGELFWIIENGVRFTGMPAFSNGGEHGGMQSSWKLVHFIRHLPHL
                     TAAERIEMERYNPKGPDDRAEEQQENDFLIRATPQRKPESQDHRQE"
     gene            2321..3559
                     /gene="cofH"
                     /locus_tag="VGR03_01665"
     CDS             2321..3559
                     /gene="cofH"
                     /locus_tag="VGR03_01665"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: protein motif:HMM:NF005609.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HEV1993012.1"
                     /translation="MATPTMNRSALTAVETCPAEVLEILHAATCGAALSFEQGLLLAT
                     AEGSALEALVAVADRLRRETVGDAITYVVNRNINFTNVCFVGCSFCGFARGPGALDAY
                     SLSFEEVVRRAREAWERGATEVCVQGGLPRDLDGFFYRDLLRAIKHAIPEMHVHAFSP
                     MEIDYGVTKTGMALRGYLQMMKDEGLGSIPGTAAEILDDRVRKELSPNKLPAARWVEI
                     ITAAHELGILTTSTMMYGHVEEPADWVRHMLLLRAIQKRTGGFTEFVPLGFIHENTRL
                     YRHGGARPGAKREEHLRVHALARVLLHGAIENLQVSWVKLGFETSLACLQAGANDFSG
                     TLMEESISKAAGATFGEYVSPEEFRARIRSIGRVPAERTTTYKIRRIFAEPEHDPRTA
                     AAELPMVRAESHVTYTEGAY"
     gene            3569..4255
                     /gene="cofC"
                     /locus_tag="VGR03_01670"
     CDS             3569..4255
                     /gene="cofC"
                     /locus_tag="VGR03_01670"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEV1993013.1"
                     /translation="MRALLLPVKDLNNAKKRLLGVLTPEERFALAGAMLADIIRAVRG
                     VRCAEKIFVVTNYEPVMQLAEENRWEILREEQQISESHSVDAASKICEQRGVSGLLRL
                     PLDLPLIQSSDIDALLAEECQAPALVIVPSRDGTGTNAILRTPPTLFPSHFGNGSFAK
                     HLAEAERAHARVMLRRNARLEMDVDDEADLRALLEHDLSGTETGRWLRASGVEAKFLP
                     NMRAGAMSAR"
     gene            4252..4929
                     /locus_tag="VGR03_01675"
     CDS             4252..4929
                     /locus_tag="VGR03_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEV1993014.1"
                     /translation="MKEKLYASLDEVVADIPDGAQIMFGGFGGAGFPNNLIQALARKG
                     TKNITAISNNCGTREGELGLMFKNNQIKHVIASFPGPHANYFQERFAAKEVTLELVPQ
                     GILCERMRAAAAGLQGFYTTVGVGTEVAEAKEERVLEGQRCILELPIHADYALIKAQQ
                     ADELGNLTYRLAARNFNPIMAMAATTTIAEVEEIVPVGAINPEHVMTPAIFVHRIVQA
                     KGLRYAG"
     gene            4949..5653
                     /locus_tag="VGR03_01680"
     CDS             4949..5653
                     /locus_tag="VGR03_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEV1993015.1"
                     /translation="MPGDGIEKKVMPAKPRLTREQIAQRAALELPDGAYVNLGWGIPN
                     LIADYLPKEITVYFHSENGILGMGRRAKPGEEDFDQVDAMKVPVTLIPGASFFHQADA
                     HLMTRGGHLDVAVLGGFQVSEKGDLANWKIPGAKGSGGIGGAMDIAAGAKTLIVCMEH
                     TTKDGTPKIVKQCTYPLTGLACVDTIVTDLAVIEVKPEGLILREVARGWTPEEVQQLT
                     GAQLIVRQRIPEMSFA"
     gene            5672..6070
                     /locus_tag="VGR03_01685"
     CDS             5672..6070
                     /locus_tag="VGR03_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEV1993016.1"
                     /translation="MSQVIPEKYADLLEKQAFGNLGTLMKDGSPQVTPVWVDYDGKFV
                     RINSAKGRVKDKNIRRDPRVSISLQDPANPYRYLEIRGRVVEITENGADDHINKLSQK
                     YLGNPVYPFRKPGEVRVTYKIEPEKVSSMG"
     gene            complement(6199..6825)
                     /locus_tag="VGR03_01690"
     CDS             complement(6199..6825)
                     /locus_tag="VGR03_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993017.1"
                     /translation="MFFSGPKSAVCAFLVLASLAPSASTGQTKREKSVWNYDGGVFFA
                     TDGSLPNGVCFRVSGGMNSPEFFLNLKRIDGERGVIFRRGTETVTHFPGDLLVSFDIH
                     DQPCTPGLREIEARTYMTQEMMRSLHLSFYWKRGVDLRPVKNITNVRASVEPIVPYAA
                     NLASELPERFDWSYQLAVPSEGVPLTDSLVLVFRTPGGRIAARVAARL"
     gene            complement(6845..7753)
                     /locus_tag="VGR03_01695"
     CDS             complement(6845..7753)
                     /locus_tag="VGR03_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1993018.1"
                     /translation="MLNPSFLSDSQTEKPEWCRALPRPEYKTLQRVLPDDSWFEVYKV
                     SPGVFAIYEPHQAEEVISYLIVGHKQAVLFDTGMGISDIRKVTTKLTSRPVVVLNSHT
                     HDDHVGGNWQFTFVYGMDTEFTRTNAKGSREDAQAEITPDQLCGVLPKGFNPKTYATK
                     PWKISHAIHDGFKINLGGRTLEVLSTPGHTPDAISLLDRENGLLFTGDTYYPAPIWLY
                     RPETDLDAYVNSVTRLAALAPELKLVLGAHNIPVAEPSVLPRLVAAIQAVRSGQGTVK
                     PEGAGKAIHSLDGFSFLLAAPKQEKD"
     gene            complement(7960..9057)
                     /locus_tag="VGR03_01700"
     CDS             complement(7960..9057)
                     /locus_tag="VGR03_01700"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEV1993019.1"
                     /translation="MTLNSGYSAVRRDGVRNRHSLQCVAALLLFACPIAVQQAAAQQA
                     GYKLKLKPTPKTVVWGYYDAKAAPVLRVKSSDTVEIQTLITNSPKDLEAAGVSPEQVE
                     QSLRDIYDQVKDKGPGPHILTGPVFIEGAEPGDVLEVRILAVKLAISYAYNGFWPGLG
                     YLPDDFPYAKIKIIPLDEKRMVAKFAPGIEIPLHPFFGSMGDAPPETSGRLSSNPPWI
                     QAGNLDNKDLVAGATLYIPVHAPGALFEVGDGHAGQGDGEVDITALETSLIGTFQFVV
                     RKDMHLKWPRAETPTHYITMGLHEDLNEATKLAVREMIDFLMTEKHLSRDDAYMLASV
                     AADLHITELVDGNKGVHMMLPKSIFVSGSSK"
     gene            complement(9054..9809)
                     /locus_tag="VGR03_01705"
     CDS             complement(9054..9809)
                     /locus_tag="VGR03_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993020.1"
                     /translation="MSKATADLPTSWSLHWLGAARTSVVIPMETGKYYEVQLTNHGAP
                     PDAKILTVDCAPQGGEGGYLTALEWHPNSSGRSVGTVVRLIAVCLTEEPVASTRRVLV
                     SVVWIRGEESDAWPYLVTAFGAAAAQEFAPSMVFAQSAVEISMMPLIEQRLRRHVAAN
                     RVENFMKDSLTYSHALNVVLPYLIAELGGPKLPEPIRGQLNSLRKKRNAIIHQGVKAA
                     AVSREDAMKGLCAAAFGFEYMRHVGPILSESHR"
     gene            complement(9986..11401)
                     /gene="fumC"
                     /locus_tag="VGR03_01710"
     CDS             complement(9986..11401)
                     /gene="fumC"
                     /locus_tag="VGR03_01710"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEV1993021.1"
                     /translation="MSEAAKPAASIPTRTESDSMGKIEVPIDRYYGAQTARSLIHFAI
                     GKDTMPSELIRAFGILKKAAALVNQDLGKLSHEKCSLIMRAADEVIDGKLNEHFPLRV
                     WQTGSGTQTNMNVNEVISNRAIEIAGGEMGSKKPIHPNDDVNMSQSSNDTFPAAMHIA
                     AATETARRLLPAVKNLRDALDAKAKEFADIVKIGRTHLQDATPLTLGQEFSGYVNLLD
                     RDGGRVAVALDGLYDLAIGGTAVGTGLNAHPEFADRAARKVSELTGLLFRSHPNKFAA
                     LSAHDEIVFASGALKTLAASLMKIANDIRWLASGPRCGLGELTLPENEPGSSIMPGKV
                     NPTQCEALTMVAVQVMGNDAAIGFAGSQGNLELNVFKPVMIFNYLHSVELLADACNSF
                     VEHCVHGIAANREQIDRYVQNSLMLVTALAPKIGYDQAAQVAKAAHHSHISLREAALK
                     LGYVTAEEFDALVKPEDMTHP"
     gene            12016..12195
                     /locus_tag="VGR03_01715"
     CDS             12016..12195
                     /locus_tag="VGR03_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993022.1"
                     /translation="MIKPLPNVLDLPLEERAVMALNSAIEEVIKEHAREGLPLYIWRD
                     GQVVTVPAEELRPRS"
     gene            complement(12203..14632)
                     /locus_tag="VGR03_01720"
     CDS             complement(12203..14632)
                     /locus_tag="VGR03_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1993023.1"
                     /translation="MQSLGSDLRYAARELRKRPGFTLTAVLSLALGIGATSAVFSVIY
                     AVLIDPFPYPGSDRIMEYRLVDKAGNDRYAGLTGPQMDQLRQAKSLESVVGMDFWNLT
                     TTDGDLPEDVQSIHISPNAPNHWGIPALKGRWLIPSDAPPGEEPQRVVVLGYQFWQRY
                     FLGDPNIIGRKMQLVHKTYEIVGVMPPRFKWGEGEIYVPLHVTNDPHIHFATSLKLRP
                     GITAAQASAELQPIMEEFAKQSPERYPEKFRVGLRSIIEVYARPLGPTLYLLLGAVAS
                     LLLIGCGNVSILLLARGAERQHELAVRAAVGADRLRIIRQLLTESLAIAGAGTVLGVL
                     LAWKSLSLLTKFLPQGSFPAESVIKMNVPVLLFSIALAVATAVVFGLWPALQLSRPDI
                     ARLMQSSMRRMTASAHARRTHSAMVGAQVALTLLLLTVASAAGKGFLRLVKTDLGYDP
                     GHTMSVPIPIHDNTHVSWQDRSEYFEQLRAKIAAMPDVVAAGISTNATPPSNGWDTRI
                     EISGNNGEKPEARVNFISPEYFPLLRIPLAQGRIWDHVETMHGAPLVVINQTMARQYW
                     PNGNAIGQQIRIPDLKGEPPYSPAAPGSDGWLQIVGVVADARNDGLRNKIKPSMYVPY
                     PLRLRMFTQILVRTQVAPMSILRDIRAQLVQVDPDQQVMRVRDLQTWITGQTEYAQQR
                     LVATLFGIFSILALVLAAVRLYSVVSYGVATRTNEFGIRMALGAEAADVFRIVLSSTA
                     VNVGAGLAVGLLLSIAFDKLATKWVMESSRDPLILTGVTLLLIVAAGLACFVPARRAA
                     SVDPMEALRYE"
     gene            14855..15931
                     /locus_tag="VGR03_01725"
     CDS             14855..15931
                     /locus_tag="VGR03_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV1993024.1"
                     /translation="MAEETKVARATAKTRLRRRLRFLRTRARELKMIGKALVSTKHPI
                     LVHIIPMRQCNLACTYCNEFDDFSKPVPLEEMKKRLDALADMGTSIITISGGEPLMHP
                     DLDEVIRHMRRRGMIAGLITNGFYLNKERIERLNGAGLEHLQISIDNVVPDEVSKKSL
                     KTLDGRLVMLAQYAVFQVNINSVLGSGVKDPEDALKIAHRAVELGFTNTVGIIHDNNG
                     QLKPLGPHEQEIFEEVMALGKRSFSRFNEFQHSVARGREHNWRCRSGSRYLYICEDGL
                     VHWCSQQRGYPGIPLSQYTPEMRHREYSTKKFCAPRCTVSCVQQVGILDNWRDPQNLK
                     PMPMTPAASPPAELVQIGQVRGDS"
BASE COUNT         3407 a         4143 c         5184 g         3199 t
ORIGIN      
        1 tggagcttcc ggagccggca aatccacgct gctggattgc atcgcggggc tggcgcggcc
       61 gaacgagggg cgaatcgtgg ccggcggcga tgtactgttc gattccgcgc gtggaatcca
      121 tgttcccgcg ccgaagcggc gaatcgctta cgtttttcaa accctcgcac tctttccgca
      181 tctgagcgcg gaagagaatg ttgtctacgg attgagggat ttgcgggagg aagaaaggca
      241 ggagcgagta ggagcaattc tcaaagcctt tcgcgtcgag aaattaagga aacaaaagcc
      301 cggcgagatt tccggaggcg aaaggcaaag aatcgcgttg gcgagatcac tggtgaccga
      361 gccgcgcgtg ctgctgctcg acgagccgct gaccggattg gacgcggaac tgaaaacggc
      421 gattgtggac gatttgcgcg cgtggaatac ggcgaaggga atccccatta tgtacgtgac
      481 gcacagccgc gaagaagtgg atgcgctggg cgagcgcgtg atcgcgctcg acaatggacg
      541 cgtcgcaagc accggtgcac cgatggaggt actggacgcg ccgcgccgga aaaggctggc
      601 ccaagccgcg ggattcgaga atctgctcag ctctaccgtg ctcgatctgc gcgaggcgga
      661 cggtgtgatg cgcgttcgct tgggcgagag cgcgtgtgag atcgaggtac cgctcggcta
      721 tgcggcagcc gggaaccgcg tgcaggtagc ggttcgcgcg ggagatattt tgctggcgac
      781 cgaacggccg cacggattga gcgcgcgaaa cgtcatggag gggcgcatcg tttcgctgga
      841 gcagcgcggg ccgatggtca ttgcgcgcgt ggattgtggc gtcacgttcg ttgtgcacat
      901 cacgcccggc gcagtgcgct cactggagct aacggccgga cggagcgttt ggctggtatt
      961 gaaaacacac tcgtgccatc tggtggatta gaagcgaagt ttaagaacaa gaaactcttc
     1021 aagatttcgg cttcgctgga gcgggctttg cgggttttgc cgcaaccggg acggaaaact
     1081 tgatttcgtc agcctcaaga gtgttgtcct tgggtgtggc gtggatgacg acgaggtcac
     1141 cggcggcgag gtcgccggcc ttggctggtt tgtcctcatt gctggatctc gaaacaaaga
     1201 cggtcgaagc ggcgaatttc acttccacgg acttcccatc cgcggttttt acaaacaagg
     1261 aatcgacgct gattttctcg accgtgcctc tcacgtgaac tttattgccg tgcgcgaatg
     1321 ccgcgcccgt aaccagcaca aacaataaag ccagcgtgga taagcgaagt ttcatgaaag
     1381 tctcccatcg gaatgaagtt ccggcttgcg atggcttcat tcctggcgat gatcttgtga
     1441 ttccggcttt cgttgtggtg ttgctctaat caagaaatca ttttcctgct gttcttccgc
     1501 ccggtcgtcc gggcctttgg gattatagcg ctccatttcg atgcgttccg cggcggtgag
     1561 atgaggaaga tggcggatga aatgcaccag tttccaactg ctctgcatgc ctccgtgctc
     1621 gccgccattg gaaaatgccg gcattcccgt gaaccgcacg ccgttctcga tgatccagaa
     1681 cagctctcca tccgtaagat tctgcgtttc cggcaaacgc agatcaggcg gcttggggta
     1741 gaggcccttg ccaatcatcg tctgtccgcc gccgtcgttg gcgtggcaga cggcgcaatg
     1801 atccgcgaaa tgcagccgag cgtcgcgcag atcttccgcc gaggcgagaa agggattctg
     1861 cgccagccgc gcattcgaag ggatcgcgag gtggcgcgca ttgcgagcga ccatgatctc
     1921 gaatttattg ggcgtggcgc gagagctaag gccatcatgc aggaatgacg cggccacagc
     1981 gacgacgagg ccgaggagcg ctacagcaac gatgatcacg agagttctgc gtttcatggc
     2041 cctgtaagta tgttaatgga tgagggccgg agcggccagc ggcgaatcat ccggcgcaga
     2101 cgccacaatg cagcatggct tcatcccggc tcgcgcgtag tcgcggtgca taggggaatg
     2161 gcgagaaagc gactcttttc ctatgatgta aagggtttgt gttttattcg aatcgaagcg
     2221 gtgtgcgaat atcgggcatt ttcagaagca ctgcgcggga attgaaagag cctgcggatg
     2281 tagccccgcg agcggtttcc tctggtagga tactcaggaa atggcgacgc ccacgatgaa
     2341 cagaagcgcg ttgacggcgg tggaaacctg cccggcagaa gttcttgaaa ttctccacgc
     2401 cgcgacgtgc ggcgcagcgc tgtcgttcga acagggactg ctcctggcca cagcggaagg
     2461 ttcagcgctc gaagcgctag tggccgttgc agatcggttg cggcgcgaaa ccgtgggaga
     2521 cgcaatcact tacgtcgtaa atcgaaacat caacttcacc aacgtgtgtt ttgtgggatg
     2581 cagtttctgc ggcttcgcac gcgggcccgg tgccctcgac gcctactcgc tctctttcga
     2641 agaagtggtg cgccgcgccc gcgaagcctg ggaacgcggc gccacggaag tttgcgttca
     2701 gggcggcttg ccgcgcgacc tcgacggatt tttctaccgg gatctgcttc gcgcgatcaa
     2761 gcatgccatc cccgagatgc acgtgcatgc gttctcgccc atggagattg attacggcgt
     2821 gacgaaaaca ggaatggcgc tacgcggcta cctgcagatg atgaaggacg aggggctggg
     2881 cagcattccc ggcaccgcgg cggaaatcct ggatgatcgc gtacggaagg aactgagccc
     2941 aaacaaattg ccggcggcgc gctgggtgga aatcatcacc gcggcgcacg agctgggcat
     3001 tctgacaact tcaacaatga tgtacgggca cgtggaagag ccggcggatt gggtgcgaca
     3061 catgctcctc ttgcgcgcga ttcaaaagcg caccggtgga tttacggagt tcgtgccgct
     3121 gggattcatc catgagaaca cgcggctcta ccgtcatggt ggagcgaggc cgggagcgaa
     3181 acgggaagag cacctgcgag tgcacgccct ggcacgggta ctgttgcacg gagcgatcga
     3241 gaacttgcag gtgtcctggg taaagttggg tttcgaaacg tcgctcgcgt gcttgcaagc
     3301 aggcgcaaac gatttcagcg gcacgctcat ggaagagagt atttccaagg ccgccggcgc
     3361 aactttcgga gaatatgtgt cgccagaaga atttcgcgcc aggattcgat cgattgggcg
     3421 agttcccgcg gaacggacga cgacctacaa gattcggcgc atatttgctg agccggaaca
     3481 cgacccgcgg acagcggcgg cggaactccc gatggttcgg gcggagagcc acgtgacata
     3541 caccgaaggc gcctactgag cgattcccat gcgcgcactc ctcttaccgg tcaaagacct
     3601 gaacaatgcg aagaagaggt tgctgggtgt gctgacgccc gaagaaaggt ttgcgctggc
     3661 cggggccatg ctcgcggaca tcattcgcgc agtgcgtggc gtgcgctgcg cggaaaagat
     3721 ttttgtcgtt acgaattacg agcccgtcat gcagttggct gaggaaaatc gctgggagat
     3781 tctgcgcgag gagcagcaaa tttccgagag ccattccgtg gatgccgctt cgaaaatctg
     3841 cgagcagagg ggcgtcagcg gattgctgcg tctgccgctc gatctgccgc tcatccaatc
     3901 gagcgacatc gatgcactcc tggccgagga atgccaggcg ccggctttgg tgattgtgcc
     3961 gtctcgggac ggcaccggca cgaacgccat actgcggacg ccgccgactt tgtttccctc
     4021 acattttggg aacggaagtt tcgcgaagca tcttgccgaa gccgaaaggg cccatgcccg
     4081 ggtgatgctg cgccgtaatg cccggctgga gatggacgtt gacgacgaag cagatttgcg
     4141 ggcactgctc gagcacgatt tgagcggaac ggaaacggga cgctggctgc gcgcgagcgg
     4201 cgtcgaggcg aagtttctgc caaacatgcg ggccggagcg atgagcgcgc gatgaaggaa
     4261 aaactatacg cgagtctaga cgaggtcgtg gcggatattc ccgatggcgc gcaaattatg
     4321 ttcgggggat tcggaggcgc gggctttccg aacaatctga ttcaggcgct ggcgcgcaaa
     4381 ggcacgaaga acatcaccgc catcagcaac aattgcggga cgcgcgaagg cgagcttggg
     4441 ctcatgttca agaacaatca gattaaacac gtgattgctt cctttcccgg gcctcacgcc
     4501 aactattttc aggaacggtt cgccgcgaag gaagtgaccc tggaacttgt gccgcaggga
     4561 attctgtgcg agcgcatgcg cgccgccgcg gccggtctgc aaggcttcta caccaccgtg
     4621 ggcgtgggca ccgaagtcgc cgaagcaaag gaagagcgcg tccttgaagg ccagcgctgc
     4681 attttggaat tgccgattca tgccgactat gccttgatca aagcgcagca agccgatgag
     4741 ttggggaatc tgacctatcg gttggccgca agaaatttca atcccatcat ggccatggcc
     4801 gcgaccacga caatcgcgga agtggaagaa atcgtgccgg tgggtgccat taatccggaa
     4861 catgtcatga cgccagcgat cttcgttcat cgcatcgtgc aagcgaaagg actccgttat
     4921 gccgggtgac ggcgttcgga aaaaggacat gccgggtgat ggcattgaga agaaggtcat
     4981 gccggctaag ccgcgattga cgagggaaca gatcgcgcaa cgcgccgcgc tcgagttgcc
     5041 ggacggcgcc tatgtgaatc tcggctgggg aattcccaat ctcatcgcgg actatttgcc
     5101 gaaagaaatc accgtctatt tccacagcga aaatggaatt ctgggaatgg ggcgccgcgc
     5161 aaaacccggc gaagaagatt tcgatcaggt cgacgcgatg aaagtgcccg tgacgctgat
     5221 tccgggagct tctttctttc atcaggcgga cgcgcatctc atgacccgtg gcggccacct
     5281 cgatgtcgct gttttgggcg ggtttcaagt gtcggagaag ggcgacctgg cgaactggaa
     5341 aattcccggc gcgaaaggct ccggcggtat cgggggcgcg atggacatcg cggcaggcgc
     5401 gaagacgctg atcgtgtgta tggagcacac tacaaaagac ggcacgccga agatcgtgaa
     5461 gcagtgcacc tatccgctga ccggattggc gtgcgtggat accatcgtca cggatctagc
     5521 ggtcatcgag gtgaagccgg aaggattgat cctgcgcgaa gtggcgcgcg gctggacgcc
     5581 ggaggaagtg cagcaattga ccggcgcaca attgatcgtg cggcagcgaa ttccagagat
     5641 gagtttcgct tgatttgatg aggaggccaa aatgagccag gtgattccag aaaaatacgc
     5701 ggatttgctg gagaagcagg cgttcggaaa tctggggacg ctgatgaagg acggaagccc
     5761 gcaggtgact cccgtgtggg tggattacga cgggaaattc gtgcggatca attcggcgaa
     5821 gggccgcgtg aaggacaaga acatccggcg cgatccccgc gtttccattt cgttgcaaga
     5881 tcctgccaat ccctaccgct acctggaaat ccgcggccgc gtcgtggaaa tcaccgaaaa
     5941 cggcgcggac gaccacatca acaaactctc gcaaaaatat ctggggaatc cagtctatcc
     6001 cttccggaag ccgggcgaag tgcgcgttac ctacaagatc gagccggaaa aagtcagttc
     6061 catgggctga cgtggtcatc ctgcgaggaa cctcctctcg gcggggctga gatacgatca
     6121 tcccttgccg atagctcacg ttcatgaccc caagttccgt gactgaaaac taaaaactaa
     6181 aaacttgcca actgtaactc agagccgcgc ggcgacccgc gcggcgatgc ggccaccggg
     6241 cgtgcggaat acaagcacaa ggctgtccgt gagtggaacg ccctcgctgg gaacagccag
     6301 ttgataagac cagtcgaagc gctcgggcaa ctcagacgcc agattggcgg cgtagggcac
     6361 gatgggttcg accgacgcgc ggacattggt gatgttcttc acggggcgca aatccacgcc
     6421 gcgtttccaa taaaaagaaa gatgaagact ccgcatcatt tcctgagtca tgtacgtgcg
     6481 cgcttcgatt tcgcgaagtc cgggcgtgca tggctgatcg tggatatcga aagacacaag
     6541 aagatcgccg ggaaaatgcg tgacagtttc cgtgccgcgc cggaagatga cgccccgctc
     6601 gccatcgatg cgcttaagat tgaggaagaa ttcgggagaa ttcatgccgc cggacacgcg
     6661 aaaacaaacc ccattcggga gcgagccatc ggtcgcgaag aagactccgc cgtcatagtt
     6721 ccaaacagat ttttcacgct tggtctgacc tgtggacgcg ctcggcgcca gtgaagccaa
     6781 gaccaggaac gcacagacgg ccgatttggg gccagagaaa aacatagggg atcaaattca
     6841 aaaactagtc tttctcctgc tttggtgcag ccagtagaaa agagaagccg tcaaggctgt
     6901 ggatggcttt gcccgctcct tcgggtttga cagtgccctg gccggatcga actgcttgga
     6961 tggcggcgac aagtctcgga agcaccgaag gttcggccac cgggatgttg tgcgcgccta
     7021 gcacgagttt cagttccggg gcgagagccg cgagacgcgt tacggaattg acgtaggcat
     7081 cgagatcggt ttccggccga tagagccaga tgggggcggg atagtaggtg tcgccggtaa
     7141 agagcagtcc attctcgcgg tcgagcagcg aaatcgcgtc cggcgtatga ccgggcgtgg
     7201 atagaacctc cagagtgcgg ccgccgagat tgatcttgaa tccatcatga atcgcgtgag
     7261 agatcttcca gggttttgtt gcgtacgttt ttggattgaa cccctttggc aaaacgccgc
     7321 agagctgatc gggagtaatt tccgcttgcg catcttcgcg tgaacctttc gcattcgtcc
     7381 gcgtgaactc ggtgtccatg ccgtagacga aagtgaactg ccagttgcct ccaacgtgat
     7441 catcgtgcgt gtgggagttg agaaccacga cgggacgcga cgtcaatttg gtcgtgactt
     7501 tgcgtatgtc gctgatgccc atgccggtgt cgaagagaac agcttgcttg tggccgacga
     7561 tgagatagga aatgacttct tccgcttgat gcggttcgta aatggcgaag acgcccggcg
     7621 agactttgta gacctcgaac caagaatcgt ccggaaggac gcgctggagg gttttgtatt
     7681 ctggacgggg aagcgcgcgg caccattcgg gtttttcggt ttgcgagtca gagagaaaag
     7741 atggatttaa cacagagcgc acagagatcg gagcacagag aacacagaga aataacatca
     7801 gcgactggaa gaaaatgagc ggggactgga cggctttccg aaaaaggccg aaaggtgtca
     7861 ttccgagggc cgtagttgcc cgaggaatct gctctttccc ggcgcacttc cgaaaagcag
     7921 attcctcgct tcgctcggaa tgacaatctt gcgctttgtt catttggagg acccagaaac
     7981 gaaaatggat ttgggaagca tcatgtggac gcctttgttg ccgtcgacga gttcggtgat
     8041 gtggagatcg gcggcgacac tggcgagcat gtaggcgtcg tcgcgcgaaa gatgtttttc
     8101 ggtcatcagg aagtcgatca tctcacggac ggcgagcttg gtggcttcat ttaagtcttc
     8161 atggaggccc atggtgatgt agtgcgtggg cgtttcggcg cgcggccact tcaggtgcat
     8221 gtccttgcgc acgacgaact gaaacgtgcc gatgagcgaa gtttcgagag cggtgatgtc
     8281 cacttcgccg tcgccctggc cggcgtggcc gtcgcccact tcaaagagcg cgccgggcgc
     8341 gtgcacggga atgtagagcg ttgcgcccgc gacgagatcc ttgttatcga gattgcccgc
     8401 ttgaatccac ggcggattgc tgctgaggcg gccggaagtt tccggcggcg cgtcgcccat
     8461 gctgccgaaa aagggatgca gcggaatctc gatgcccggc gcgaatttcg ccaccatgcg
     8521 cttttcatcc agcggaatga ttttgatttt cgcataagga aaatcgtccg gcaggtatcc
     8581 caggcccggc cagaaaccgt tgtaggcgta ggaaatcgcg agcttcacgg cgaggattcg
     8641 cacttccagg acgtcgccgg gctccgcgcc ttcgataaat accgggccgg tcagtatgtg
     8701 cggaccggga cccttgtcct tgacctggtc gtagatgtcg cgcagcgatt gctcgacttg
     8761 ctctggtgag acgccagcgg cttcgaggtc tttggggcta ttggtgatga gagtctgaat
     8821 ttccacggta tcactcgact tgacgcgcag caccggcgcc gccttcgcgt cgtagtagcc
     8881 ccacaccacc gtcttcggcg tcggcttcag cttcagcttg tatcccgcct gctgcgccgc
     8941 tgcctgctgg acggctattg ggcatgcgaa caggagtaat gcggcgacac attgcagcga
     9001 gtggcggttt ctaacaccat cacggcgtac ggcgctatat ccagaattaa gggtcatcgg
     9061 tgcgactccg ataagatcgg tccaacgtga cgcatgtact caaagccgaa ggctgcagcg
     9121 cagaggccct tcattgcgtc ttcgcgcgaa acggcagctg ctttcacacc ttgatgaatt
     9181 attgcattcc gtttttttcg cagactgttg agctgtccac gaatgggctc gggcagcttt
     9241 ggcccgccta gttcggcgat caggtaaggg agcacaacat ttagagcgtg actataagta
     9301 agtgagtcct tcatgaaatt ctctacgcgg ttagcggcta cgtggcgcct taggcgttgc
     9361 tcgattaacg gcatcatcga aatctcgacc gcagattgcg caaagaccat cgacggcgcg
     9421 aattcctggg ccgcggcggc gccgaatgct gtcactagat acggccatgc atcggactcc
     9481 tccccgcgaa tccagactac gctgacgagg actcgacgag tgcttgcgac aggttcctcc
     9541 gtaaggcaca cagctatcaa acggacgact gtccccacgc tgcgacctga tgagttgggg
     9601 tgccattcta gtgccgtcag gtatccacct tctccgccct gcggcgcgca atccactgtg
     9661 agtatcttgg catcaggcgg agcgccgtgg tttgttagct gcacttcata atacttgcca
     9721 gtttccatgg gaatgactac ggaagtcctc gccgcaccga gccagtggag gctccacgaa
     9781 gttggtagat cggccgtagc tttgctcaag acggctttcc atagatcgac gcttttccgg
     9841 caggatacac agactatttg gccatccttg aagtaagtgg gcgcgcactc cacaagacgg
     9901 ggcaacacat aatgacattc gggacaagaa ccacaatatt ccatcgtgtc gtcgaattca
     9961 ggattttttg gcgttgacga tgtgctcatg ggtgggtcat gtcctcgggc ttgacgaggg
    10021 cgtcgaattc ttcggcggtg acgtagccga gtttgagggc ggcttcgcgg aggctgatgt
    10081 gggagtggtg ggcggctttg gcgacttggg cggcctggtc gtagccgatt ttgggagcga
    10141 gggcggtaac gagcatgagg gagttttgga cgtagcggtc aatctgttcg cgattggcgg
    10201 cgatgccgtg gacgcagtgc tcgacgaagc tgttgcaggc gtcggcgagg agttcgacgg
    10261 agtggagata gttgaaaatc atgacgggct tgaaaacatt tagttcaagg ttgccctggg
    10321 agccggcgaa gccgatggcg gcgtcattgc ccatgacttg cacggcgacc atggtgaggg
    10381 cttcgcactg cgtgggattc actttgccgg gcatgatgga agagccgggc tcgttttcgg
    10441 gaagcgtgag ttctccgagg ccgcagcgcg ggccggaggc gagccagcga atgtcgttgg
    10501 cgatcttcat gagtgaggcg gcgagcgttt ttaatgctcc agaggcgaag acgatttcgt
    10561 cgtgcgcgga gagcgcggcg aatttattgg gatgcgagcg gaaaaggaga cccgtgagtt
    10621 cggagacttt gcgcgcagcg cgatccgcaa attcgggatg ggcgttgagg ccggtgccga
    10681 cggcagtgcc gccgatggcg agatcgtaga ggccgtcgag agccacggcg acgcgtccgc
    10741 cgtcgcggtc gagaagattc acataaccgg aaaattcctg gccgagcgtg agcggcgtgg
    10801 cgtcctgcaa atgggtgcgg ccgattttga cgatgtcggc aaactccttg gctttggcat
    10861 cgagcgcatc gcgaagattt tttacggcgg ggagcaggcg gcgcgcggtt tccgtggcgg
    10921 cggcaatgtg catggcggcg ggaaacgtgt cgttggagga ctgcgacata ttgacgtcat
    10981 cgttcggatg aatgggcttc ttgctgccca tttcgccgcc ggcgatttcg atggcgcggt
    11041 tggagatgac ttcgttgacg ttcatgttgg tctgcgtgcc gctgccggtt tgccagacgc
    11101 gaagagggaa gtgctcgttg agctttccgt cgatgacttc atcggcggcc cgcatgatga
    11161 ggctgcactt ttcgtgcgag agctttccca agtcctggtt cacgagcgcg gcggctttct
    11221 tgaggatgcc gaaggcgcgg atgagctcgg acggcatagt gtccttgcca atggcgaagt
    11281 ggatgagcga gcgggcggtt tgtgcgccgt agtagcggtc gatggggacc tcgatcttgc
    11341 ccatggagtc ggactcggtg cgagtcggga tggaggctgc tggcttggct gcttcagaca
    11401 taggggactc cttgataggt tgctgccctg attctagcag caagtggttg gtgactcgtg
    11461 actggtgaca aatggaggag acagagtaag gcctcagccc tgaaagctgc gctacaggat
    11521 tgagagggcg atttctagtt aacaatttac cccccacccc ccactttttt tggatgtgtg
    11581 gcaaataaag gagttatgga cgcatttttc ggatgtgtgg caaataaaga acttagaaga
    11641 aggtggatag tgatcagtga aaagtggcca gtggaggaaa ttgaaaaaag attcttgggc
    11701 acatcgaatc tccggaagtc gtccagtgag ttctcttcgc acaaatcgct cggggtgaac
    11761 tcttttcaaa atgggaagag ttgggggtat acccccggtg ttttgcgtat gagtggcaaa
    11821 caaaggggtt agtggctggc gaggggggat agagaagtag caggaagctg ggagagaccc
    11881 aggaagaggg agaagcgagc agctcacatc atattatagc atatgggtac aatttgtcaa
    11941 ggattaatag taagtggttt ggatgctgtg agttacaaaa gggcatgatg atgtagttgc
    12001 tcgttacgga gcacgatgat caaacctctg cccaacgttc ttgacttgcc gctggaagag
    12061 cgcgcggtca tggcacttaa ttcggcgatc gaagaagtca tcaaagaaca cgcccgcgag
    12121 ggattgccgc tctatatctg gcgcgacggt caagttgtga cggttcctgc cgaagagctg
    12181 cgtccgcgat cctgaacgcc ggctattcgt agcgcagagc ttccatggga tcgacggaag
    12241 ccgcgcggcg ggcgggcacg aaacaggcca ggcccgcggc gacgatgagc aagagcgtca
    12301 cgccagtgag gatgagcgga tcgcgcgagc tttccatcac ccactttgtg gcgagcttgt
    12361 cgaaagcgat gctgagcaga agacctacag cgagcccggc acccacattc actgccgtcg
    12421 aagacaggac gatccgaaat acgtcggccg cttccgcgcc gagggccatg cggatgccga
    12481 attcattggt gcgggtcgca acgccgtagg acacgacgct gtaaagcctg acggcagcga
    12541 ggaccagcgc aagaatcgag aagatgccga atagggtggc gacaaggcgc tgctgtgcgt
    12601 attcggtctg gccggtgatc caagtttgca gatcgcgaac gcgcatcacc tgctggtccg
    12661 ggtcgacctg aacgagctgg gcgcgaatgt cgcgcagaat cgacatcggc gcgacttggg
    12721 tgcgcaccag aatctgggtg aacattcgga gccgcagcgg ataaggcaca tacatggagg
    12781 gcttgatctt gttgcgcaag ccgtcgttgc gcgcatccgc gacgacgccg acgatttgca
    12841 gccagccgtc gcttcccgga gcagccggcg agtaaggtgg ctcacctttg aggtcgggaa
    12901 tgcgaatttg ctgcccgatg gcgttgccgt tcggccaata ctgccgcgcc atggtctggt
    12961 tgattaccac gagcggggcg ccatgcatgg tttccacgtg atcccaaatc cggccctgcg
    13021 ccaggggaat gcgcaggaga ggaaagtatt cagggctgat gaagttcacc cgggcctccg
    13081 gcttctcccc gttgttccca ctgatttcaa ttcgagtatc ccagccattc gagggcggcg
    13141 tggcgttggt ggaaatgccg gcggcgacga catcgggcat cgcagcaatc ttggcgcgga
    13201 gttgctcgaa gtattcagac cggtcctgcc aggaaacgtg ggtgttgtcg tggatgggaa
    13261 tggggaccga catcgtgtgg ccggggtcgt aaccgagatc ggtctttacg agccgcagga
    13321 aacccttgcc cgcggcactg gccacggtga gcaggagaag tgtgagcgcg acttgagcgc
    13381 cgaccatcgc gctgtgggtg cgccgggcgt gcgcgctcgc cgtcatgcgc cgcatactgc
    13441 tttgcatcag ccgcgcgatg tctggccgcg agagttgcaa ggccggccag aggccaaaca
    13501 cgacggcggt agcgacggcc agagcgatgc tgaagagaag gaccggcacg ttcattttga
    13561 taaccgattc ggcgggaaag gaaccctgcg gcaggaattt tgtgagcagg gaaaggctct
    13621 tccatgccag gagaactccg agcacggtgc cggccccagc gatggcgagc gactcggtga
    13681 gcaactgccg gatgatgcgg aggcggtcgg cgcccacggc ggcgcgcacg gccagttcat
    13741 gctggcgttc ggcacctcga gccaagagca gtatggatac gttcccgcag ccgatcagga
    13801 gcaaggaagc taccgcgccg agcaggagat agagcgtcgg tccaaggggc ctcgcgtaga
    13861 cctcgataat acttcgcagg ccgacgcgaa atttttccgg ataacgctct ggggattgtt
    13921 tcgcgaactc ttccattatg ggctggagtt cggcactggc ctgggcggca gtaatacccg
    13981 ggcggagctt cagggaagta gcgaaatgga tgtgcggatc gtttgtgacg tgcaacggga
    14041 cgtaaatctc tccctcgccc catttgaaac ggggcggcat caccccgacg atctcatagg
    14101 tcttgtgaac gagctgcatc ttgcggccaa tgatattggg atcgcccaga aaatatcttt
    14161 gccaaaactg gtaaccgaga acgacgacgc gttgcggctc ctcgccaggc ggtgcgtcgg
    14221 agggaattaa ccagcggccc ttgagcgcgg gaatgcccca atggttggga gcgttcgggg
    14281 aaatgtggat ggattgtacg tcttccggaa ggtcgccgtc cgtggtggtc agattccaga
    14341 aatccatgcc gacgacactt tcgagggatt tggcttggcg aagctgatcc atctgcgggc
    14401 cggtcaagcc cgcgtagcgg tcattgccgg ccttgtccac gaggcgatac tccatgatgc
    14461 gatcggagcc agggtacggg aacgggtcaa tcaggaccgc atagatgacg ctgaagacgg
    14521 cgctggtggc tccgataccg agagcgaggg agagtacggc ggtcagcgtg aagcccggcc
    14581 gcttacgcag ttcgcgggct gcatagcgca aatcagaccc cagggattgc attagcacct
    14641 cgcatggcta ccgcttcgac cttgtcgacc tttgtgggat ttgacgatgg aggagcggcg
    14701 atggttagtt gacggctggc accgattgag cctttttttg agaaatccgg aatgagtgaa
    14761 tgacttgcgg tgggcaagag tacgagtggg gcaatagggc agcggcgttc agcgcggtca
    14821 tgtataattt ttggcagggg attctgcttc ttccatggct gaagaaacaa aggtagcgcg
    14881 ggcgactgcg aaaacacggc tgcgaagacg gctgcgtttc ctgcggacgc gcgcgcgcga
    14941 gctcaaaatg attgggaagg cgctggtgtc gacgaagcac cccatcctgg tgcacatcat
    15001 tcccatgcgg caatgcaatc ttgcctgcac gtactgcaac gaattcgacg atttctcgaa
    15061 gccggtgccg ctggaagaaa tgaagaagcg gctggacgcg ctggcggaca tggggacgtc
    15121 gatcatcacg atcagcggag gcgaaccgct gatgcatccg gacctggatg aagtgatccg
    15181 gcacatgcgg cggcggggca tgatcgccgg gttaatcacc aacggtttct atctgaacaa
    15241 ggaacgcatc gagcggttga acggggcggg gctggagcat ttgcagatca gcattgataa
    15301 cgtggtgccg gacgaagtgt ccaagaaaag cttgaagacg ctggatggcc ggctggtgat
    15361 gctggcgcag tacgcggtgt tccaggtgaa tatcaactcg gtgctgggca gcggcgtgaa
    15421 agatccggag gatgcgctga agatcgcgca ccgggccgtg gagctgggct tcaccaacac
    15481 ggtggggata attcacgaca acaacgggca attgaaaccg ctggggccgc acgagcagga
    15541 aatttttgaa gaagtgatgg cgctgggaaa gcgttcgttt tcgcgattca acgaatttca
    15601 gcacagcgtg gcgcgcgggc gggaacacaa ctggcggtgc cgctcgggct cgcggtattt
    15661 gtatatttgc gaagacgggc tggtgcactg gtgctcgcag cagcgggggt atccggggat
    15721 tccgctgtcg cagtacacgc cggagatgcg ccaccgcgag tactctacca agaagttttg
    15781 cgcgccgcgg tgcacggttt cgtgcgtgca gcaggtgggg atactggata actggcgcga
    15841 cccgcaaaac ctgaagccca tgccgatgac gccggcggca agcccgccag cggagctggt
    15901 gcagatcggg caggtccgcg gcgactcctg att
//
LOCUS       DASYVO010000047         5470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_15664, whole genome shotgun sequence.
ACCESSION   DASYVO010000047 DASYVO010000000
VERSION     DASYVO010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15664"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VGR03_01730"
     CDS             <1..287
                     /locus_tag="VGR03_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV1993025.1"
                     /translation="QEQTFDAEQKKYQLGASTVFLVIQTQRDLTTAQGNELRALVDLV
                     KAKADYERALGRTLNANRVTVADGQSGSVERDTLIPGTLHGEVVGTQKTY"
     gene            complement(574..987)
                     /locus_tag="VGR03_01735"
     CDS             complement(574..987)
                     /locus_tag="VGR03_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993026.1"
                     /translation="MRVLLLLAALMCFGVSTAVAQDPVVADADHIKLEFENSQVRVLR
                     YSYKPGEKSPIHDHLTNVLIALTDQDAKSTANGKTTDFHAKAGQVFWRNATKHVFENV
                     GTTPADGILVELKGGPSASKAAKTKAAKTKAAKTK"
     gene            complement(1489..2385)
                     /locus_tag="VGR03_01740"
     CDS             complement(1489..2385)
                     /locus_tag="VGR03_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993027.1"
                     /translation="MRRMLLPLAVIAFIASAEAQKKAAPIPAGGGDIACGNQHYIVPG
                     LCLQKCTGRRTLANLNAGQCFFAPQCKALQGDQTLKQFYNQGILHSGGPQVLPPDVAQ
                     RSVVAQIINVHYKPFNAGQFDIRIGNFAGKDAWAGIERGGGKGSKPILGVSPDLLLLT
                     PGFLASVLGHEMVHESQYRRKYKTNMTGINSAVGAFRELEASMWELGEASSPGFPDNK
                     FNDCLQESESTSNRQTRDCRSWQVTKAIENIRDSPRSYFKSLEAWMNEDPWVSAEWIP
                     KNPNWKMAKAGPNPDPKECDNP"
     gene            2787..4505
                     /locus_tag="VGR03_01745"
     CDS             2787..4505
                     /locus_tag="VGR03_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEV1993028.1"
                     /translation="MAEQDKGAIQTAPSPETPLVRREPSDQERAWEEKTLNPTLARSP
                     ERLPEFTTISAHPIRRLYTEADLAGWDAEKELGYPGQPPYTRGIHSTMHRGRLWTMRQ
                     FAGFGTAEDTNRRFRYLLTQGQTGLSTAFDLPTLMGYDSDHPLSEGEVGKCGVAISSL
                     ADMEVLFDKIPLAEVTTSMTINSPAAVIWAMYLAVAEKQGADWKKISGTIQNDILKEY
                     IAQKEYIYPPEPSMRLVIDTFEFGMKNTPRFNTISISGYHIREAGSTAIQELAFTLRD
                     GMEYVEWGMRRGMDVDEFVPQLSFFFNAHNDFFEEIAKYRAARRIWHKAMVERYKAKS
                     PRTWALRFHSQTAGCSLTAQQPYNNVVRTALQALAAVLGGTQSLHTNSLDEAWALPTE
                     FAATIALRTQQIIAHETGVTNTVDPLGGSYFVETLTNEVERGAWEYIERIDAMGGMVA
                     AIERGYPQREIAEASYRYQLAVDHKEKIVVGVNDYVAEEKPLETLQLDETVAHRLTER
                     LARLRAERSNDEVARRLAALRKAAVGKENLMPFLYEAVKAYATLGEVCDAMRDVFGIY
                     EEVAIT"
     gene            4680..5051
                     /locus_tag="VGR03_01750"
     CDS             4680..5051
                     /locus_tag="VGR03_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEV1993029.1"
                     /translation="MSDNELTGAIIGAAIEVHRHLGPGLLESAYEECLFHELLSQEIR
                     VERQKPVPVVYKGKKLECGYRMDLLVDGRVVVELKSVETLASIHEAIILTYMKLGECR
                     LGLLINFNVEMLKDGVRRFIL"
     gene            5143..>5470
                     /locus_tag="VGR03_01755"
     CDS             5143..>5470
                     /locus_tag="VGR03_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEV1993030.1"
                     /translation="MPEKKIRVLIAKPGLDGHDRGAKVIARALRDAGMEVIYTGLRQT
                     PEMIASAAVQEDVDVIGLSILSGAHNTLCPQLMALLRDKGMTDVTVLVGGIIPEADIP
                     GLKKAGI"
BASE COUNT         1129 a         1647 c         1594 g         1100 t
ORIGIN      
        1 tacaggaaca aactttcgac gcggaacaga agaagtacca actcggcgcc tcgacggtct
       61 tcctggtcat ccagacgcag cgggacctga cgacggcgca ggggaacgag ctccgcgcgc
      121 tggtggatct ggtcaaggcc aaggcggact acgagcgcgc cctgggaaga acgcttaacg
      181 cgaaccgcgt gaccgtcgcc gacggccagt cgggctccgt cgagcgcgac accctgatcc
      241 cgggaacgct ccacggagaa gtcgtcggga cgcagaaaac gtactaagct gggatgcacg
      301 gcatggagat tgcaaccgac gctagtctca gctcttcagc cctgaaggtg ttaacgcttt
      361 taagttggga tgcacgcaac tatcgagact cgagtagcag ttagcaggcc caatttgctt
      421 agacgattgc tacgaaaact gacgagccgg gtttcaatgc agtcttttac cgtcgcactc
      481 gcggcagcgg gcgcaagagg gatctctgcg cctcgccgat atacaagaat cctgagagag
      541 ttggccgggc gctggagggc taattaagcc cagtcacttg gtttttgccg ccttggtctt
      601 tgccgccttg gtttttgccg ccttggatgc gctcggccca cctttcaact ccaccagaat
      661 tccatctgcg ggggtggtgc caacgttctc gaacacgtgt ttcgtagcgt tcctccaaaa
      721 cacctgtcca gctttcgcat ggaaatccgt tgttttgcca ttcgcggtgg acttcgcatc
      781 ctgatccgtt aacgcgatta aaacattggt cagatggtcg tgtatcgggg atttttcgcc
      841 gggtttgtaa ctgtaccgca atacccgcac ctgggaattt tcaaactcca gcttaatgtg
      901 gtccgcatcc gcgacgaccg gatcttgcgc taccgcagtg gacaccccaa aacacatcaa
      961 cgccgctagt aaaagcaaca cgcgcatgtg acacctccgt aagtggcggg aattatacag
     1021 cgttttgttt tcatgatctc agcccgggtc atgttaactg gaatttccgt tttgagtttc
     1081 ccctttgtcc gaaagcctag ccctcctgcc ccgtcgtgtc aaccgcaaat cgcgctggcc
     1141 cctttgggtg gtagtgcggc tcgttttggc cgttctgcgg agccgctgtg ctggttttgc
     1201 ccgccccagt ctgcctcgcc gctgacactt ccccgctgga tggcttccga gcgctcgttt
     1261 gctttctgtc gtggttcgct aaccgtccgg cagcggcaac gccgcgatca ttctcagcat
     1321 gctgccgaac agcttccgac tcagatgtcc ctcggccagc aaaagccaat aatagcgcgc
     1381 atgtctcacc agccgaccac gcctggcacc agccgcgcca ccgacgagag tagtcgtccc
     1441 ctaattcctg ctactggccc cctcagatct ggggctcgtc ccgcgacgct aggggttgtc
     1501 gcactccttc gggtccgggt tcgggcccgc tttcgccatc ttccaattcg gattcttggg
     1561 aatccactct gcactgaccc acggatcttc attcatccag gcttcgagcg acttgaaata
     1621 cgaacgcgga gaatcgcgta tgttctcgat cgccttcgtt acctgccacg agcggcaatc
     1681 gcgcgtctgc cgattggaag tgctctcgct ctcctgcaga caatcgttga acttattgtc
     1741 aggaaaacct ggcgatgaag cttcccctag ctcccacatc gaagcttcca actcgcggaa
     1801 tgcccccacc gccgagttga ttccggtcat gttcgtcttg tacttgcgac ggtactgcga
     1861 ctcgtgcacc atttcgtgcc cgagcacgct ggccaggaaa ccgggcgtca acaagaggag
     1921 gtcgggcgag acgcccagga ttggcttgct ccctttccct ccgccgcgct cgattccagc
     1981 ccaagcgtcc ttgcccgcaa agtttccaat gcgtatatcg aactgcccgg cattgaacgg
     2041 cttgtagtga acattgatga tctgcgccac caccgagcgc tgcgccacgt ctggggggag
     2101 tacctggggg cctccgctgt gcaggatgcc ctggttgtag aactgcttga gcgtctgatc
     2161 gccctggagc gccttgcact gaggcgcaaa aaaacactgc cctgcgttca ggttcgccag
     2221 ggtccttcgc cccgtgcatt tctgcaggca tagtcccgga acgatgtagt gctggttgcc
     2281 gcaggcaatg tcgcctcctc ctgcgggtat aggcgctgcc ttcttctgcg cctccgccga
     2341 cgcaatgaaa gcaatcacgg ccaacggcag caacatgcga cgcatattcg agcccccctc
     2401 gggttatatc ttgagaacgg atcagagtag ataagcggca catccacaca tgcgggttcc
     2461 gagtgcgtgc atcacacata ctgttcgcgt cattttgaaa ctcctctctc gttgaaacct
     2521 gttgtctgaa cgctcagcat ttgcctgacg aagcatccat cgcgtctcgt cgcttaaaat
     2581 acatcttcca accgtttagt ttgggaaagg ttatcacgaa gcgggcagag caaactactt
     2641 acccacccgg gcgcagcccc ttgctttttc tctttcgcag gatgtcatcg ctttgcttgt
     2701 ccttccgcgc tctttcgact accataaaat cgtccccgtg gagacgggct ccagattcca
     2761 tctccgcgcc cggagagcgc caacccatgg ctgagcagga caagggagcg attcagaccg
     2821 caccatcccc ggaaaccccg ctcgttcgcc gcgaaccatc cgaccaggaa cgcgcctggg
     2881 aggaaaaaac cctgaacccg acgctggcca ggagtcccga gcgcctgccg gaattcacga
     2941 ccatctccgc gcatccgatc cggcggctct ataccgaagc cgacctcgcc ggctgggatg
     3001 cggagaaaga gctggggtac ccgggccagc cgccgtacac gcgcggcatc cattcgacga
     3061 tgcaccgcgg gcggctttgg acgatgcgcc agttcgcggg cttcggcacg gccgaagaca
     3121 ccaaccggag attccgctac ctgctcacgc aaggtcaaac gggactctcc acggcgttcg
     3181 atctgccgac gctgatgggc tacgactccg accacccgct gtcggaggga gaggtcggca
     3241 agtgcggcgt ggccatcagc tcactcgccg acatggaagt cctcttcgac aaaatcccgc
     3301 tcgccgaagt gacaacctcg atgaccatca attcccccgc cgcggtcatc tgggcgatgt
     3361 acctggccgt cgccgaaaag cagggagcgg actggaagaa aatctcgggg acgattcaaa
     3421 acgacatcct caaggagtac atcgcgcaga aggaatacat ctacccgccc gagccttcga
     3481 tgcggctggt gatcgacacg tttgaattcg gcatgaagaa cacgccgcgg ttcaacacca
     3541 tctccatcag cgggtatcac atccgcgaag ccggctcgac ggccatccag gagctggcgt
     3601 tcacgcttcg ggacggaatg gagtacgtcg aatggggaat gcggcgcggg atggacgtgg
     3661 atgaatttgt gccgcagctc tcttttttct tcaacgctca caatgatttc ttcgaggaga
     3721 ttgcgaagta ccgcgcggcg cggcgcatct ggcacaaggc gatggtcgaa cgctacaagg
     3781 cgaaaagccc gcgcacctgg gccttgcggt tccactccca gacggcgggg tgctcgctga
     3841 cggcccagca gccgtacaac aacgtggtgc gcacggcgct gcaggcgctg gcggcggtgc
     3901 ttggcggaac gcagtcgctg catacgaact cgctggacga ggcgtgggcg ctgccgacgg
     3961 aatttgccgc gacgatcgcg ctgcgcacgc agcagatcat cgcgcacgaa accggcgtga
     4021 caaacacggt ggatccgctg ggcggttctt atttcgtgga gacgctgacg aatgaagtcg
     4081 agcgcggcgc gtgggaatat atcgaaagga ttgacgccat gggcggcatg gtggccgcaa
     4141 tcgaacgcgg atacccgcag cgggagatcg cggaagcttc gtaccgctac cagcttgccg
     4201 tggaccacaa agagaaaatc gttgtcggcg tgaacgatta cgtcgcggag gaaaagccgc
     4261 tcgagaccct gcagctcgac gagacggtag cgcaccggct gaccgagcgg ctcgcgaggc
     4321 tgcgcgccga acgttccaac gacgaggtcg ccaggcggct ggccgcgctg cgcaaggcgg
     4381 ccgtgggcaa ggaaaacctc atgcccttcc tctacgaagc ggtgaaggcc tacgcaaccc
     4441 tgggcgaagt gtgcgatgcc atgcgcgacg tcttcggaat ctacgaagaa gtggccatca
     4501 cttaggccag cgtttctccg gcgtgctgtg gccatgaatg tctttgccca aagtgcggat
     4561 tgcaggcgct ggattcttca ccgcattgga cctcgactcg cacatgcttc gccttctctg
     4621 tgatctctgt gccctctgtg gtgaattttt tcccttttgg ctgtgataga gtgcggcaca
     4681 tgtccgacaa tgaattgacg ggcgcgatca tcggcgcagc gattgaagtg catcgccatc
     4741 tcgggcccgg cttgctcgag tccgcctacg aagagtgctt gttccacgag ctgcttagcc
     4801 aggagatccg cgtcgagcgt cagaaaccgg tgccggtggt ttacaaggga aagaaattag
     4861 aatgcgggta ccgaatggac ctgctggtcg atggccgggt ggttgtggag ctgaaatccg
     4921 tcgagacgct tgcttccatc catgaagcga tcatcctcac ctatatgaag cttggcgaat
     4981 gcaggcttgg tctgctgatt aacttcaatg ttgaaatgtt gaaggacggg gttcgacggt
     5041 ttatcctcta gcgaaccgaa agaatgggaa gaagaaacaa ggcaaaccaa agaagaagcc
     5101 accacagaga acacggagat cacagaggaa gagaagagga gaatgccgga gaaaaagatc
     5161 agagtgctga ttgcgaagcc ggggctggac gggcacgacc gcggcgccaa ggtgattgcc
     5221 cgggcgctgc gcgacgcggg catggaagtg atctatacgg ggctgcggca gacaccggaa
     5281 atgatcgcct cggccgcggt gcaggaggac gtggacgtga tcggcctttc catcctctcc
     5341 ggcgcgcaca acacgctctg cccgcagttg atggccctgc tgcgcgacaa gggcatgacc
     5401 gacgtgaccg tcctggtggg cggcatcatt ccggaagccg acattcccgg cctcaagaag
     5461 gcaggcattg
//
LOCUS       DASYVO010000048        15364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_43947, whole genome shotgun sequence.
ACCESSION   DASYVO010000048 DASYVO010000000
VERSION     DASYVO010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 15364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15364
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43947"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VGR03_01760"
     CDS             complement(<1..533)
                     /locus_tag="VGR03_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEV1993031.1"
                     /translation="MARIVRCGLIQAQCEWSPEKFSLAEIKKKMIAKHERLIAGAAKQ
                     KVQILGLQELFYGPYFCAEQQTRWYELTEKVPNGPTVARMQKLAKKYQMVLVVPVYEV
                     EMTGVYYNTAAVIDADGRYLGKYRKHHIPHCHPGFWEKFYFTPGNTGYPVFQTRYARV
                     GVYICYDRHFPEGARILG"
     gene            647..2338
                     /locus_tag="VGR03_01765"
     CDS             647..2338
                     /locus_tag="VGR03_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993032.1"
                     /translation="MQRTAAGLTLWTARTTIKGKRISVRSPNVGRKGRWVIALLWAFC
                     LLLLPSGRVASAQEHGAHGAKGALGTVEFRVSCTAEAQQSFTRGVALLHSFTYEESAE
                     AFRNAAAKDPHCAMAHWGLAMTEYHQMWEPYAGPAELQRGSAEIRKARELKPGTPREK
                     DYVEALGVFYDGWEQRGHAARAKAYGDAMRGVHERNPKDQEAAIFYALALVATATPED
                     KTYDNQRKAAAILEPLFAAQPDHPGVAHYLIHAYDNPVLAPQGVTAARAYSKIAPDLP
                     HALHMPSHIFTRLGLWEDSISSNIASAAAARKHGDQGEEFHALDYLVYAYLQLGRNDE
                     AEKLRDHLPTVGILEPYSVFKINFAKAAIRARCALEQQHWTEAENLVAEPGVQPQVAA
                     ISHWAAALGAAHTGNLQAARQHAEQVRRLAIQLEEKGEAYWAQQVAIQGEEAGGWVAF
                     AEHRTYDALRLLRMAADHEDAAEKHPVTPGAVRPARELLGDLLLEMHQPKEALVAYRQ
                     VLTVAPGRRNALKGAAEAERMAGMSSKLRRIPAVPSASGSIESGTLAPQVPVAGR"
     gene            complement(2631..2972)
                     /locus_tag="VGR03_01770"
     CDS             complement(2631..2972)
                     /locus_tag="VGR03_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEV1993033.1"
                     /translation="MSEHIVSPKIYIAIFACLMLGTLITVWAAFQNYGKFNIVIALAV
                     ATIKATLVVLYFMHARYSPKRTQLVIVCSVFWLAILLALTLADYDTRAHDTQPGVGRF
                     APPAASHFFTA"
     gene            complement(2984..3742)
                     /locus_tag="VGR03_01775"
     CDS             complement(2984..3742)
                     /locus_tag="VGR03_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HEV1993034.1"
                     /translation="MHRTGELYGHFQTLEQQKESATLGMWIFLVTEVLFFGGIFLAYT
                     INRTAHSIAFGAGSNTLDIRLGGGNTVVLILSSLTMAMAVWSAQVGKKRWVSFFLIAT
                     LALGTVFLGVKVIEYKQKFDHHLIPGHNFDITYCVNNPAACGNPAELAAEKKELEEAV
                     KAEYPSDPAIGLVALNAHAQLYFSLYFGMTGLHALHMIVGAGLLLWLIKGSFSGRFTP
                     QYNTPVEVVGLYWHFVDIVWIYLFPLLYLIDRHR"
     gene            complement(3875..5554)
                     /gene="ctaD"
                     /locus_tag="VGR03_01780"
     CDS             complement(3875..5554)
                     /gene="ctaD"
                     /locus_tag="VGR03_01780"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEV1993035.1"
                     /translation="MSNTPPTPLPGHKLPQENYLNATYGLRSWLLTTDHKRIALLYLG
                     SVTFFFFIGGIFATMIRIHLLTPTGALVTPETYNKLFTMHGVAMIFFFLIPSIPAVLG
                     NFLIPMMIGAKDLAFPKINLLSWYIYIVGGCFVLYSFGTGGLDTGWTFYTPLSSVYSN
                     SAVTPAVLGIFINGFSSILTGLNFIVTIHTMRAPGMTWFRLPLFAWAHYATSLVMILG
                     TPVLAITLLLAGLERMFHLGFFNPALGGDPILFQHLFWFYSHPAVYIMILPAMGVISE
                     IIPTFARKPIFGYSFIAGSSIAIAVIGFLVWAHHMFVTGESPYAALTFSFLSYLVAIP
                     SAVKVFNWTSTLYKGSISWDTPMLYAFGFIGLFLIGGLTGLFLACLGLDIHIHDTYFV
                     VAHFHYIMVGGAVMGYVGGLHFWWPKITGRLYPEVLSRIAAVTLFVGFNLTFFPQFIL
                     GYLGMPRRYHTYPPEFQVLNVLSSAGASILALGYLLPMLYLTWSMRYGKVAGQNPWPA
                     TGLEWKTDSPPPTENFHVTPEVTWEPYDFQNRPDLGLAAGAPSPVPAHGDD"
     gene            complement(5558..6541)
                     /gene="coxB"
                     /locus_tag="VGR03_01785"
     CDS             complement(5558..6541)
                     /gene="coxB"
                     /locus_tag="VGR03_01785"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEV1993036.1"
                     /translation="MQSQLPIYPEQASNFAPHVDSLMLFITAVCLFFAVAVTTAIVIF
                     FFKYHRKAPNAVGIPIHEDSRLEAVWMIVPLILAMAMFSWGAVIYVDYRRAPLDTLDI
                     YVIAKQWMWKAQQPSGLKEINELHVPVGRNIRLIMASEDVIHDFFVPAFRVKMDVVPG
                     HYNTMWFRPTKPGRYHFFCSQYCGTNHALMGGWVTVMEPSDYAAWLSGSSGGNTNPVV
                     AGEKLFAEKACVTCHVPNGTGRSPSLNGVYGAQVLLADGSTVVADEAYIRESILQPNA
                     KIVAGYQPLMPTFQGQLTEEQILALTAYIKSLQSQPVPVKGAGIAPPTGKK"
     gene            complement(6545..7426)
                     /locus_tag="VGR03_01790"
     CDS             complement(6545..7426)
                     /locus_tag="VGR03_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEV1993037.1"
                     /translation="MTKARHIVGIAALLLAASTLHAQTIPGNVGPSAATMPRALQNVG
                     FEPPLNGPMPLELAFRDETGRSVQLREYFGQQKPVVLAFVYYGCPMLCDQVEQGVVGV
                     LRMLSFNPGRDYEVVFVSFDSRETPEMAAEKKKKALVHFRRPETDSGWHFLTGSKESI
                     EAAAKAANFRFTFDAKSNLFAHAAGVMVLTPDGHISRYFYGVEYPGRDMRLGLVDASA
                     GKIGAPIDHVLLFCYHYDPTAATYSASILKIIRLGGVLTILCIVGGILISRRRETLAA
                     ARKLRQPLSSGVERGAH"
     gene            complement(7431..7886)
                     /locus_tag="VGR03_01795"
     CDS             complement(7431..7886)
                     /locus_tag="VGR03_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993038.1"
                     /translation="MSTESHNTGQEPRHTDVSFEERDIKARAIYGYLFALGAAVVASL
                     LICVFILRFTLNFAASSDTPPPPSREALGKDFRALPPEPRLQGVPGHETDPQADLRKK
                     NQEDGEANEKLEWIDKSGGIAQIPVKDAMKIVAEKGLPAAPAPAAEKKK"
     gene            complement(7876..9063)
                     /locus_tag="VGR03_01800"
     CDS             complement(7876..9063)
                     /locus_tag="VGR03_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993039.1"
                     /translation="MSAPDYRAPESVGRLQQWALSIGGVALLVSILGAVRTPELFYQS
                     YLMIFLLILGLTVGSLGLLMLQHLTSGHWGIIIRRPLESATRTLPLIVAFFLPILFFG
                     MKYLYGAWLKPEALEKEPLSQFQQTYLTSGRFQVRAVIYFAIWLVLMFIFNSLSKQQD
                     TNQADRALRLRLKMLAGPGIILYVFVMTFAAIDWVMSLSPHWASTIYGFLFVAGQLIS
                     SMSLMIAVVVLLARTEPLASVLQKRHLHDLGKLLLAFVMLWAYFDFSQLLIIWSGNQP
                     EEITFYRTRLYGGWGVVAVIVLVFHFFVPFFLLLSQDVKRNAKVLPKIAIWLIFMRLV
                     DLFWMTRPEFTSRAMPTWLDVVLPIALGGLWLGLFAFNLKQRPLLPLGDPELAEAIEH
                     HEH"
     gene            complement(9060..9713)
                     /locus_tag="VGR03_01805"
     CDS             complement(9060..9713)
                     /locus_tag="VGR03_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEV1993040.1"
                     /translation="MTNTSRFLVSACSACLVFGAGCDYLRQDMANQPKNRPLSPSSFF
                     EDGRSERPLVENTVERGALTNDELFVPKESNNFPLPVNLDLLERGEERYKIFCTPCHG
                     LQGDGNGMVAIRGMKQPPTYHQDRLRQAPNGYFYDNITNGFGQMLGYSAQIPPRDRWA
                     IIAYIRALQLSRNAKAADLPAELREKLKASGTAGDIPGGAKPGQIGSAKEGMGEKKE"
     gene            complement(9706..10248)
                     /locus_tag="VGR03_01810"
     CDS             complement(9706..10248)
                     /locus_tag="VGR03_01810"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEV1993041.1"
                     /translation="MLHRSHLYGVMGEFGDPEELIHAVEKIREAGYRRVNAYSPFPVE
                     GLSEALGLKRNMVPLICLLGGLAGGVGGFFFQYWAAAIAYPLNVGGRPLNSWPAFIPV
                     TFELTILGASLAAVFGMLALNKLPQPHHPVFNVPRFTHASSDRFFVCIEAKDPKFHLA
                     ECARLLQEVHAHHVTEVSDD"
     gene            complement(10248..11633)
                     /gene="nrfD"
                     /locus_tag="VGR03_01815"
     CDS             complement(10248..11633)
                     /gene="nrfD"
                     /locus_tag="VGR03_01815"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEV1993042.1"
                     /translation="MSAGQPPFDSPEIRRLTPVIGPGHTYGSVTDKISAIVLTRPTSL
                     GWFVGFSIAFSLMGLMTVAIGWLIIKGTGIWGINIPIGWGFAIVNFVWWIGIGHAGTL
                     ISAILFLLNQKWRTSINRFAEAMTLFAVACAGIFPLIHTGRPWMAFYMFPYPSTMGIW
                     PQFRSPLIWDVFAVSTYGTVSLMFWFVGLIPDLATLRDRSVQKPMKIIYGMLALGWRG
                     SARHWHRYQSAYLLLAGLATPLVLSVHSVISFDFSVAIVPGWHTTIFPPYFVAGAIYS
                     GFAMVLTIAIPLRKAYGLEDFITMRHLDNMAKVMLATGLIVAYGYFFEFFMSSYSGNK
                     FDVFLVQQRLHGPYAPYYYALIICNILTPQLLWFYKMRHNVVVLFIMSIVVNIGMWLE
                     RFVIVVISLTRDFVPSAWGRYSPTFWDWATFIGTMGMFVTLIFLFVRVLPAISIAEMR
                     ELVAETSEDDK"
     gene            complement(11630..14794)
                     /locus_tag="VGR03_01820"
     CDS             complement(11630..14794)
                     /locus_tag="VGR03_01820"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEV1993043.1"
                     /translation="MREHEQHEHLDGHHDHNDPAAEAKLHTSPMDLAAVRAKLQSKTG
                     KQYWRTLEELAGDPEFEELLHREFPRQAPSEWDDSVDRRDFLKLMAASLAFAGLSGCG
                     RTPEQYVVPYVKQPDGMTLGKPQFYATAMPFGADAIGLLVESHEGRPTKIEGNPDHPS
                     SLGATNVFAQASVLNLYDPDRAQTVTKFGEIETWSLFVESAQALAATMKGSNGAGFRI
                     LTGLITSPTLAAQIQSLLTLFPQAKWHQWEPAVGDGTREGAKLAFGVYLNTVYRPEKA
                     DVILSLDSDFLGSGPGHIRYSREFSRRRKLNSPTDTMNRLYVVEPTPSVTGATADHRL
                     PLRATEVEQFARALAAKFGLGGSAELPTGAEKWLDAVAKDLQGHKGASLIVAGEHQPA
                     EVHALAHAINAALGNVGTTLFYTRSVEERPVNHVESLRELCADLDSGKVETLLILGVN
                     PVYTAPHDFDFASKMKFDTKTNSKKVKNTIYVGSHFDETAELCDWHIAESHYLETWGD
                     ALAFDGTLTVIQPLIAPLYHTHSAREVLAAFGDKPGISDYDTLRDRLKSTNPFADFEK
                     FWRKSLNDGLVSSSAYPSVSVPLKFSAASLPAAKSAPAGEIEFIFRPDPCVYDGRFAN
                     NGWLQELPKPVTKLTWDNAALVSPKTAERLGLTHDVGARGGEHGKIYSNVIDISLSNS
                     KVTAAAWRLPGQADGVVVLPLGYGRKRAGYTGTNKGFNAYAVRTSGALWTASAPSSAI
                     KKTGGDYPLACTQYHFNMEGRQILATGTLEEYRKNPNFANEHAEAPKNELSLYNGEAE
                     FPYKRDKWAMAIDLNKCNGCNACVVACQSENNIPVVGKDQVMRGREMHWIRIDRYYEK
                     AVKSATNDPSSYDESLFNPPTFFQPVPCQQCENAPCEQVCPVGATVHSAEGLNDMVYN
                     RCVGTRYCSNNCPYKVRRFNFLRFQDWETPQLKLLRNPEVTVRSRGVMEKCTYCVQRI
                     NNARIESEKLNAPIHDGAIVTACEQACPTEAIVFGNANDPSSRVAKLKAQQRNYTLLG
                     ELNARPRTTYLAAVRNPNPELESA"
     gene            complement(14803..>15364)
                     /locus_tag="VGR03_01825"
     CDS             complement(14803..>15364)
                     /locus_tag="VGR03_01825"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEV1993044.1"
                     /translation="VARSPYMTRQNVSREQPVQFSHKHHVGDDGIDCRYCHTGVETSA
                     VAGIPPTKTCMNCHSVLFNNAAYLEPIRESYRSDKSIQWVKVHRLADFVYFDHSIHVN
                     KGVGCSSCHGQISQMPLVFQANTLLMQWCLDCHRNPEQVLRPKDQVFNMDWKAPDDQV
                     QIGAKLKVEYNIRTTAELTSCSTCHR"
BASE COUNT         3463 a         4197 c         4811 g         2893 t
ORIGIN      
        1 ccgaggatgc gcgcgccttc ggggaaatgg cggtcgtagc agatgtagac gccgacgcgg
       61 gcgtagcgag tctggaagac ggggtagcca gtgtttccgg gcgtgaagta aaacttttcc
      121 caaaatcccg ggtggcagtg aggaatgtgg tgcttgcggt atttgccgag gtagcggccg
      181 tcggcgtcga tgaccgcggc ggtgttgtag tagacgccgg tcatctcgac ttcatagacg
      241 gggacgacga gcaccatttg gtatttcttc gcaagcttct gcatccgcgc gaccgtgggg
      301 ccgttgggga ccttctcggt cagctcgtac cagcgagttt gctgctcggc gcagaagtac
      361 gggccgtaga agagttcctg aaggccgagg atctgtactt tttgtttggc ggcgccggca
      421 atcaggcgct cgtgcttggc gatcattttc tttttgattt cggcaagcga aaacttctcc
      481 ggagaccact cacattgggc ctgaatcagg ccgcagcgaa cgatacgggc catgccacac
      541 ctcccgagca accgcaggcg aaaagtctac tccgtgcgtg attgggcgac aaggttttgg
      601 cagccgactg tgccacttca gcggcgtcaa caatccgatg cagtgaatgc aacgaaccgc
      661 tgccggattg acgctctgga ctgcgcgcac taccatcaag ggcaaacgaa tttccgtgag
      721 gagtccgaac gtgggaagaa aaggtagatg ggtaatcgca ctgctatggg cgttctgctt
      781 gttgttgctg ccaagcggca gagtcgcgag cgcccaagag catggggccc atggagcgaa
      841 gggagccctc ggaaccgtcg agtttcgggt gtcgtgcacc gcggaggcac agcaatcgtt
      901 cacgcgcggt gtggcgctct tgcattcgtt cacctacgaa gaatccgcgg aagcgtttcg
      961 caatgccgcg gcaaaagatc cgcattgcgc catggcgcat tggggactgg cgatgacgga
     1021 ataccaccag atgtgggaac cgtatgccgg gccggcggaa ttgcaacgcg gctccgccga
     1081 aattcggaag gcgcgcgaat tgaagccggg aacaccgcgc gaaaaggatt acgtcgaggc
     1141 attgggggtt ttctatgacg gttgggagca acgcggccac gcggcgcgcg ccaaggctta
     1201 tggagatgcg atgcgcggag tgcatgagcg aaatcccaag gatcaggaag cggcgatttt
     1261 ctatgcgctg gcgctggtgg cgacggctac gccggaagac aagacgtatg ataatcagcg
     1321 aaaagcggcg gcgatcctcg agcccttgtt tgcggcgcaa cccgatcatc ctggagtcgc
     1381 gcactacctg atccatgcct acgacaatcc ggtgctcgcg ccgcaggggg tgacggcggc
     1441 acgcgcctat tcgaagatcg caccggatct tccgcacgcg ctgcacatgc cgtcgcacat
     1501 ttttacccgg ctgggattat gggaggattc gatttcgtcg aacatcgctt cggcagctgc
     1561 agcgcgaaag catggcgacc agggcgaaga atttcatgcg ctggattatc tagtctatgc
     1621 gtacttgcaa ctgggccgga atgacgaggc cgaaaagctc cgcgaccatc tgcctacggt
     1681 tggaattttg gagccgtatt ctgttttcaa gatcaacttt gcgaaggccg cgattcgcgc
     1741 ccgctgcgcg ctggaacagc agcattggac ggaagccgaa aatctggttg ccgaacccgg
     1801 cgtgcagccg caagtggcgg cgatttcgca ctgggcggcc gcactggggg ccgcgcacac
     1861 gggaaatctg caggctgcga ggcaacatgc ggagcaggtg cgcaggcttg ccatccaatt
     1921 ggaggaaaag ggcgaggcgt attgggcgca acaagtagcg atccagggcg aggaagccgg
     1981 gggatgggtg gcgtttgcgg agcatcggac ctacgatgcg ctgcgcctac tgcgaatggc
     2041 ggcggaccat gaagatgccg cggaaaagca tcccgtgacg cccggcgcgg tccggcccgc
     2101 acgggaattg ctcggcgatc tgctgttgga aatgcaccag ccgaaagagg cgctggtcgc
     2161 ctaccgtcag gtgctgactg tggcgccggg ccgacgaaac gccctaaaag gggcagcaga
     2221 ggccgaacga atggctggta tgtccagcaa gctccgcagg atacctgcgg ttccttctgc
     2281 cagcggttcc atcgaatctg ggactttggc cccgcaggtc cccgtagcgg gcaggtaaat
     2341 ttgcaggggc ggtagagctt ttttactctg gtttgtccgg cagtctattg ataatacaag
     2401 acttagaatt ggcctggagc ttgcagttcg aaaggcagtg ggggaggcga gccgaggatc
     2461 cctttagggg attgagggct gggccggctc gatactcccc ccttttcttt catcccccgt
     2521 tggctcgcaa ctctctccga aacgttacta caaaaagact tacccaacaa attaaaagat
     2581 ggcgaatgca tgggttcgga actcgcgaat tgggcggcgt gaactgccga tcaggcggtg
     2641 aagaaatggg aagcagcggg cggggcgaag cggcccacac caggctgcgt gtcgtgagcg
     2701 cgcgtgtcgt aatcggcgag cgtgagagcc agcaggatcg ccagccagaa gaccgagcag
     2761 acgatgacaa gctgggtgcg cttgggacta tagcgggcgt gcatgaagta gagaaccacg
     2821 agcgtggctt tgatggtggc gacggcgaga gcgatgacga tattgaactt gccgtagttc
     2881 tggaatgcgg cccacacggt gatgagcgtg ccaagcatca agcacgcgaa aatggcgatg
     2941 taaatttttg gcgaaacaat gtgctcggac acgcgcgact ctcctaccgg tgcctgtcaa
     3001 ttagatacaa caacgggaac aaatagatcc acacaatatc gacgaaatgc caatacaagc
     3061 cgacgacttc cacgggggtg ttgtactgcg gcgtgaagcg gccgctgaaa gatcccttga
     3121 tgagccagag gagaaggccg gcgcccacga tcatgtgcag ggcgtgcagg ccggtcatgc
     3181 cgaaatagag ggagaagtaa agctgcgcgt gagcgttcag cgcgaccaga ccgattgccg
     3241 ggtcactggg atattccgcc ttgacggctt cttcaagttc ttttttttcg gcagcgagtt
     3301 cagcaggatt gccgcaggcg gccggattgt tcacgcagta ggtgatgtca aaattgtggc
     3361 cgggaatgag atggtgatcg aatttctgct tgtactcgat caccttgact ccgagaaaga
     3421 cggtgccgag agccaacgtg gcgatgagga aaaaactaac ccaccgcttc tttccaactt
     3481 gtgccgacca gacagccatg gccatggtca aactgctgag aatgagcacg acagtgttcc
     3541 cgccacccag tctgatatcg agcgtgttgc tgccggcgcc gaaagcgatg ctgtgcgcag
     3601 tgcgattgat ggtataggca aggaagatgc cgccgaagaa caggacttcg gtgaccagga
     3661 aaatccacat accgagcgta gcggattctt tctgctgctc gagcgtttga aagtgtccgt
     3721 acagttcgcc ggtgcgatgc acgcggtact cctcttccta agtcaatcta tggcctagca
     3781 tcggccacga gcaggacgga ctaaagtccg tccctacacc taaactccag aaagaggaca
     3841 gactaaagtc tgtcctacag cagaactcaa ctacctaatc atctccatga gccggcacgg
     3901 gcgacggcgc ccccgctgcc aagccgagat cgggccggtt ctgaaagtcg tatggctccc
     3961 aggtaacttc cggtgtgaca tgaaaatttt ccgtgggtgg cggcgaatcc gttttccact
     4021 cgaggcccgt ggccggccag ggattctgcc cggcaacctt gccgtagcgc atggaccaag
     4081 tcaggtagag catcggcagc agataaccca gcgcgagaat cgatgcgccc gcggaggaca
     4141 agacgttgag cacctgaaac tccggcggat aggtgtgata gcgccgcggc attccgagat
     4201 agccaaggat gaactgtgga aagaacgtca gattgaagcc gacgaacaag gtgacggccg
     4261 caatgcgcga caagacttca ggatagagcc tgccggtaat cttcggccac cagaagtgca
     4321 ggccgccgac gtaacccatg accgcgccgc ccaccatgat gtagtggaaa tgggcgacca
     4381 cgaagtaggt gtcgtgaatg tgaatgtcca ggcccaggca ggcgaggaaa agtccggtga
     4441 ggccgccgat caagaacagt ccgatgaagc cgaacgcgta aagcatcggc gtgtcccagg
     4501 aaatagagcc cttgtagagg gtcgaggtcc agttgaagac cttgacggct gagggaatcg
     4561 ccaccaagta gctgaggaac gaaaacgtca gcgcggcata gggggattca ccagtaacga
     4621 acatgtggtg cgcccaaacc aggaaaccga tgaccgcgat agcgatgctc gagccagcga
     4681 tgaaggagta tccgaagatg ggtttgcgcg cgaacgtggg gataatctcg ctgattacgc
     4741 ccatggccgg aagaatcatg atgtacacgg cgggatgcga atagaaccag aagaggtgct
     4801 ggaacaggat gggatcgccg cccagagccg gattgaagaa acccagatga aacatgcgtt
     4861 cgaggccggc caggagcagc gtgatggcga ggaccggcgt ccccagaatc atgaccagac
     4921 tggtggcgta atgcgcccag gcgaaaagcg ggagccggaa ccaggtcatc ccgggcgcgc
     4981 gcatggtgtg gatggtgacg atgaagttga ggcccgtcaa aatcgaggag aacccgttga
     5041 taaagatgcc gaggaccgcc ggcgtcaccg ccgaattcga atacacgctg ctgagcgggg
     5101 tgtaaaacgt ccagccggta tccagcccgc cggtgccgaa ggaatacaga acgaagcagc
     5161 cgccaacgat atagatgtac caactgagaa gattgatctt cgggaaggcc agatccttcg
     5221 caccgatcat catgggaatc aggaaattgc caaggacggc gggaatcgac gggatcagga
     5281 aaaagaagat catggccacg ccgtgcatgg tgaaaagctt gttgtatgtt tcgggcgtga
     5341 ccagggcgcc ggttggcgtc agaagatgaa tgcggatcat ggtggcgaag atgccgccga
     5401 tgaaaaagaa gaacgtcacc gaaccgaggt acagcaatgc gatgcgcttg tgatccgtgg
     5461 tgagcagcca cgagcggagg ccgtaggtgg cgttcaggta gttctcctgc ggcagtttgt
     5521 ggcccggcaa tggtgttggc ggcgtgttgc tcatgactta tttcttcccc gtgggcggcg
     5581 caatgccggc accctttacg ggaaccggct gcgactgcag agacttaata taggcggtga
     5641 gagcgagaat ctgttcctcg gtcagctggc cctggaatgt gggcatcaat ggctggtacc
     5701 cagccacaat tttcgcattc ggctgcagaa tcgattccct gatgtaggcc tcgtccgcca
     5761 cgacagtgga accatccgcc agaagaactt gcgcgccata aacgccgttg agcgagggag
     5821 agcgccccgt gccattaggg acatggcacg tgacgcacgc tttttcggca aagagctttt
     5881 cgcccgcaac gacaggattg gtgttcccgc cggaggagcc ggaaagccac gcggcgtagt
     5941 cggacggctc catgacggta acccagccgc ccatcagagc atggttcgtg ccgcagtact
     6001 gcgaacaaaa gaaatggtaa cggccgggct tcgtggggcg gaaccacatg gtgttgtagt
     6061 gcccgggaac aacgtccatc ttcacgcgga acgccggaac gaagaaatcg tggatcacgt
     6121 cttcgctcgc catgatgaga cgaatgttgc ggcccacggg aacgtgcagc tcgttgatct
     6181 ccttcaagcc gctgggctgc tgcgctttcc acatccactg tttggcgatc acgtaaatat
     6241 cgagggtatc gagcggcgcg cggcgatagt cgacgtagat gaccgcgccc cagctaaaca
     6301 tggccatggc cagaatcagc ggaacgatca tccacacggc ctcgagccgc gaatcttcat
     6361 ggatgggaat accgacggcg ttcggtgctt tgcggtgata cttgaagaag aaaatgacaa
     6421 tcgccgtggt aacagcaacc gcaaagaaca ggcaaacagc ggtgatgaag agcatcagcg
     6481 agtccacgtg cggcgcgaag ttggacgctt gctccggata gattggcaat tgcgactgca
     6541 cggctcagtg agctcctcgt tccacgccac tggaaagcgg ctggcgcaat ttccgggccg
     6601 ccgccaaagt ttcgcggcgg cgggaaatca aaattcctcc gacaatgcag agaatggtca
     6661 ggaccccgcc caggcggata atcttgagaa tcgaggcgct gtaggtggcg gcggtggggt
     6721 cgtagtgata gcaaaacagc aagacgtgat cgatgggcgc gccgatcttt cccgcggaag
     6781 catccacgag tccgaggcgc atgtcgcggc cgggatattc gacgccatag aagtagcgcg
     6841 agatgtgccc gtccggagta aggaccatca cgccagcggc gtgagcgaat agattggact
     6901 tggcatcgaa ggtgaaacgg aagttcgcgg ctttggccgc agcttcaatg gattccttcg
     6961 agccggtgag gaaatgccat ccggaatccg tttctgggcg gcggaaatgg accagcgcct
     7021 tctttttctt ttcagcagcc atctccgggg tctcgcggga atcgaagctg acgaaaacaa
     7081 cttcgtagtc gcgcccggga ttgaatgaga gcatgcgcag cacgccgaca acgccttgtt
     7141 cgacctgatc gcaaagcatg gggcagccgt agtagacgaa ggcgaggacg accggctttt
     7201 gctgtccgaa atactcgcgg agctgaacgc tgcggccggt ttcgtcgcgg aaggcgagtt
     7261 ccagcggcat tggcccgttg agcggcggct cgaatcccac gttttgcaaa gcccgcggca
     7321 tggtcgcagc cgatgggccg acgttgccgg ggatggtctg cgcgtgcaga gtggacgccg
     7381 ccaggagcag cgccgcaatt ccaacgatat ggcgggcttt agtcatttcg ttatttcttt
     7441 ttctccgccg ccggcgcagg cgccgcgggc aaaccctttt cggcaacgat cttcatcgcg
     7501 tccttgacgg gaatctgcgc gataccgccg gacttgtcga tccattccag tttttcattg
     7561 gcctcaccat cttcctgatt cttcttgcgc agatcggcct gcggatcggt ttcgtggccg
     7621 gggacaccct gtagccgcgg ctccggaggc agcgctcgaa aatctttgcc aagagcctcg
     7681 cgggacggcg gaggaggcgt atcggacgaa gccgcgaaat tcaaagtgaa acgaaggatg
     7741 aaaacgcaaa tcagcaagga agccacgacc gccgcgccca gggcaaagag atacccatag
     7801 atggcgcgcg ccttgatatc gcgttcttcg aacgagacgt cggtgtggcg cggctcctgg
     7861 cccgtgttgt gagactcagt gctcatggtg ctcaatggcc tccgcaagtt ccgggtcgcc
     7921 gagcgggaga agcggccgtt gcttcagatt gaaagcaaag aggccgagcc acaatccgcc
     7981 gagcgcgatg ggcaaaacaa cgtccagcca ggtgggcatg gcgcgagaag tgaattcggg
     8041 acgcgtcatc cagaatagat ccacaagacg catgaagatg agccatatag cgatcttcgg
     8101 gagcactttc gcgttgcgct tcacatcctg cgagaggagc aggaaaaaag gcacgaagaa
     8161 atggaacacc agcacgatca ccgcgacgac gccccagccg ccgtacagcc gcgtgcgata
     8221 gaaggtgatt tcttccggct ggtttcccga ccagatgatc aggagctgcg agaaatcgaa
     8281 ataggcccag agcatcacaa aagcgaggag caattttccc aaatcgtgca ggtggcgctt
     8341 ctgcaggacg ctggcgagag gttcggtgcg cgccagcagc acgacaaccg cgatcatgag
     8401 cgacatcgag gagatcaact gtcccgccac gaacaggaaa ccgtaaatgg tggaggccca
     8461 gtgaggcgac agggacatca cccagtcgat ggccgcgaag gtcatcacaa aaacgtaaag
     8521 aatgatgccc gggccggcga gcattttcag gcggaggcgc aaggcgcgat cggcctggtt
     8581 ggtgtcctgc tgtttcgaca aactattgaa gataaacatc agcacaagcc agatggcgaa
     8641 atagatgacc gcgcgcacct ggaagcgccc gctggtcaaa taagtctgct gaaactgcga
     8701 cagcggctct ttctctaatg cttccggctt gagccaggcg ccatagaggt acttcatacc
     8761 gaaaaacagg atgggaagaa aaaacgcgac gatcagcgga agcgtgcgag tggccgattc
     8821 cagcggacgg cggatgatga tgccccagtg gccgctggtc aggtgctgca acatcagaag
     8881 gccaagcgag ccgacggtca ggccgagaat cagcaagaaa atcatcaaat aagactgata
     8941 gaaaagttcg ggagtgcgca cggcgccaag aatggaaacc agcagggcga cgccaccgat
     9001 ggatagcgcc cattgttgca atcggccgac gctctctggc gccctgtagt ctggcgcgct
     9061 cattccttct tctcccccat gccttccttg gcgctgccaa tctgtcccgg cttcgctccg
     9121 ccgggaatat cccccgcagt cccagacgcc ttcaatttct cgcgaagctc cgcgggcaga
     9181 tcggcggcct tcgcgttgcg gctgagctgc agcgcgcgga tatacgcgat gatggcccag
     9241 cgatcgcgcg gaggaatctg tgccgagtag cccagcatct gaccgaaccc gttcgtgatg
     9301 ttgtcgtaga aatagccgtt gggcgcctgg cgaagacggt cctgatgata ggttggcggc
     9361 tgcttcatgc cgcggatggc caccatgccg tttccgtcgc cctgcaagcc gtggcaggga
     9421 gtgcagaaaa tcttgtaacg ctcttcaccg cgctcgagca agtcgaggtt cacaggcagc
     9481 gggaaattgt tcgattcctt cggcacgaag agctcgtcgt tggtcagcgc accgcgctcc
     9541 acagtatttt caacgagcgg gcgctccgag cggccatctt caaagaagga cgaaggcgaa
     9601 agcggccgat tcttcggctg attagccatg tcctggcgaa gatagtcgca accggcgcca
     9661 aaaaccaggc aggcggagca agcagagacg aggaagcgcg aggtattagt catcggacac
     9721 ctctgtgaca tggtgcgcgt gaacttcctg gaggaggcgc gcgcattccg cgagatggaa
     9781 cttcggatct ttggcttcga tgcaaacaaa aaaacggtcc gacgatgcgt gcgtgaaacg
     9841 aggcacgtta aaaaccggat ggtgcggctg gggcagtttg ttcagggcca gcataccgaa
     9901 aaccgcggcg agcgatgcgc cgagaatcgt cagctcaaag gtgacaggga tgaaggccgg
     9961 ccaactattg agcgggcggc cgccgacgtt caagggataa gcgattgccg ccgcccagta
    10021 ctggaagaaa aaaccgccaa caccgccagc caggccgcca agaaggcaaa tcaacgggac
    10081 catgttgcgc ttcaggccca gcgcctccga gaggccttca acgggaaatg gcgaataggc
    10141 gttgacgcgg cggtagcctg cctcgcggat tttctccacg gcatgaatga gctcttcagg
    10201 gtcgccgaac tcacccatca cgccgtatag gtgggagcgg tgcagcatta tttatcgtcc
    10261 tcagaggtct ccgccaccag ttcgcgcatt tccgcgatgg aaatggcggg gagaacacgc
    10321 acaaaaagga agatcagagt gacgaacata cccatggtgc cgatgaaggt ggcccaatcc
    10381 cagaacgtcg gactataacg gccccaggca gaaggcacga agtcacgcgt caggctgatg
    10441 accacaatga cgaagcgctc cagccacatg ccgatattca cgacgatgga catgatgaat
    10501 agaacgacca cattgtggcg catcttgtaa aaccagagca attgcggagt caggatgttg
    10561 cagataatca gcgcgtaata atacggagca taggggccgt gaaggcgctg ctggaccagg
    10621 aaaacgtcaa acttgttgcc gctgtaagac gacatgaaaa attcaaagaa gtagccataa
    10681 gcaacgatga gtccggtggc gagcatgacc ttggccatgt tgtccaggtg gcgcatggtg
    10741 atgaaatctt cgaggccata ggccttacgc agcggaatcg cgatggtcaa aaccatggcg
    10801 aaaccggaat agatagcgcc agccacgaaa tacggcggaa agatcgtggt atgccagccg
    10861 ggaacgatgg cgacggaaaa gtcgaaacta atcaccgaat gtacgctgag cacgagcggc
    10921 gttgccaggc ccgccagcaa gaggtacgcc gactggtaac gatgccagtg acgcgccgaa
    10981 ccgcgccagc ccagcgccaa catcccgtaa atgatcttca tgggtttctg gacggagcga
    11041 tcgcgaagcg ttgccaggtc cggaatgagg cccacgaacc agaacatcag cgaaaccgtg
    11101 ccgtaggtcg aaaccgcaaa gacgtcccag atcagtgggc tgcgaaactg cggccaaatt
    11161 cccatagtgc tggggtaagg gaacatgtaa aacgccatcc acggccggcc ggtgtggatc
    11221 agcgggaaaa tgccggcgca ggctaccgcg aaaagagtca tcgcttccgc gaagcggttg
    11281 atggaggtgc gccacttctg attcaacagg aagagaatcg cggagatcag cgtgccggcg
    11341 tggccgatac cgatccacca cacgaaattg acgattgcaa aaccccaacc gatcgggatg
    11401 ttgatgcccc agatgccggt gcctttgatg atcagccagc cgatagcgac ggtcatcagc
    11461 cccatgaggc tgaaggcaat cgagaagccc acaaaccagc cgagggaagt cggacgagtc
    11521 agaacgatcg cactgatttt gtccgtgacc gacccgtagg tgtgccccgg tccgatcacc
    11581 ggggtcagcc ggcggatttc gggagaatcg aatggcggtt gcccggcgct catgccgact
    11641 ccaattccgg attcggattg cgcaccgcag caaggtaagt ggtgcgtggc cgcgcattca
    11701 gttcgccgag gagcgtgtaa ttccgctgct gcgctttcaa cttcgcgacg cggctgctcg
    11761 ggtcgttagc gttgccgaaa acgatggcct cggtcgggca ggcctgttcg cacgccgtga
    11821 cgatcgcgcc atcgtggatc ggggcgttca acttttccga ttcgatgcgc gcgttgttga
    11881 tgcgctgcac gcaataggta cacttttcca tgacgccgcg gctgcgcacg gtgacttccg
    11941 gattgcgcaa aagcttgagt tgcggcgtct cccaatcctg gaagcgcaag aaattgaagc
    12001 ggcgcacttt gtaaggacag ttgttcgagc agtagcgcgt cccgacgcag cggttataga
    12061 ccatgtcgtt caggccttcg gcgctgtgca ccgtggcgcc gacgggacag acctgctcgc
    12121 aaggggcgtt ctcgcattgc tgacacggca ccggctggaa aaatgtcggg ggattgaaca
    12181 gggactcatc gtagcttgac ggatcgttgg tcgcggactt gaccgccttt tcgtaatagc
    12241 ggtcgatgcg gatccagtgc atttcgcgac cgcgcatcac ttggtccttg ccgactacgg
    12301 gaatgttgtt ctccgattgg caagcgacca cgcaagcgtt gcagccattg catttgttca
    12361 agtcgatggc catcgcccac ttgtcgcgct tgtatggaaa ctccgcttct ccgttgtaaa
    12421 gagagagctc gttcttgggc gcttcggcat gctcgttggc aaaattcgga ttcttgcgat
    12481 actcctcgag cgtgccggtg gccagaatct gccggccttc catgttgaag tgatactggg
    12541 tgcaggctaa cggataatcc ccgccggttt ttttgatcgc gctgctggga gcggaggccg
    12601 tccagagcgc gccggaagtg cgcacggcgt aggcgttgaa acccttgttc gtgccggtgt
    12661 agcccgcgcg cttgcggcca taccccagcg gaagaacaac gacaccatcg gcctggccgg
    12721 gcagtctcca cgcggcagcg gtgactttgc tgttcgaaag cgaaatgtcg atgacgttcg
    12781 aataaatctt gccgtgctcg ccgccacgcg ctccgacatc gtgcgtcagg ccgagtctct
    12841 ccgcagtctt cgggctaacc agcgcggcat tgtcccaggt gagcttggtg accggctttg
    12901 gcaattcctg cagccaaccg ttattggcga agcgtccgtc gtaaacacaa ggatccggac
    12961 ggaagatgaa ttcgatttca ccggcgggcg cggatttcgc agccggcaag ctggccgcac
    13021 tgaatttcag ggggacgctg acggatgggt aggccgagct agagacgaga ccgtcgttca
    13081 acgacttacg ccagaacttt tcgaaatcgg cgaaaggatt cgtggattta aggcggtcgc
    13141 gaagggtgtc gtaatccgat atcccgggct tatcgccgaa agcggcaagt acttcacgtg
    13201 cggaatgcgt gtggtacagc ggtgcgatca gcggctgaat cacggtgagc gtaccgtcga
    13261 aagcaagtgc gtcgccccag gtttcgagat agtgcgattc ggcaatgtgc cagtcgcaga
    13321 gttcggcagt ctcgtcaaaa tgcgagccga cgtagatggt gttcttgacc ttcttactgt
    13381 tcgttttcgt atcgaatttc atcttggaag caaaatcaaa gtcgtgcggc gcggtgtaaa
    13441 ccggattgac gcctagaatc agaagcgtct cgactttgcc gctgtccaga tcggcgcaca
    13501 gttcgcgcag cgattcgaca tgattcacgg ggcgttcttc caccgatctc gtgtagaaca
    13561 gagtcgtccc gacgtttccg agcgcggcat tgatagcgtg cgccagtgca tggacttccg
    13621 caggctgatg ctcgcccgca acgatgagtg acgcaccctt gtgtccctgg agatctttgg
    13681 cgacggcgtc gagccatttc tcagcacctg taggaagttc cgcggaaccg cccagcccga
    13741 acttcgctgc cagcgcgcgc gcaaattgct caacttcggt tgcccgcagg ggcaggcgat
    13801 gatccgcggt cgcgccggtg acgctgggag tcggctcgac cacatacagc cggttcattg
    13861 tgtcagtcgg gctattcagt ttgcggcgcc gcgaaaattc tctcgagtat cgaatgtgtc
    13921 ctggcccgct gcccagaaaa tcggaatcga gcgaaaggat gacgtcagcc ttctccgggc
    13981 gatagactgt gtttagatag actccgaagg ccagcttcgc gccttcgcgc gttccatcgc
    14041 cgacggctgg ctcccactgg tgccacttgg cctgcggaaa aagcgtcaag agcgactgaa
    14101 tctgcgcggc gagcgtcggc gaagtgataa gaccggtcag gatgcggaaa cccgcgccgt
    14161 tggagccctt catcgtggcc gcaagggcct gcgcgctttc cacaaacaac gaccaggtct
    14221 caatttctcc gaatttcgtg accgtctgcg cgcgatccgg atcgtaaaga ttcagaaccg
    14281 aagcctgcgc aaacacgttc gtcgcgccaa ggctcgatgg atggtcaggg ttgccttcga
    14341 ttttcgtcgg ccgcccctcg tggctttcca ccagcaagcc gatggcatcc gcgccaaacg
    14401 gcatcgccgt ggcataaaac tgtggcttcc caagggtcat accatcgggc tgcttgacgt
    14461 acggcacgac gtactgctcg ggcgtgcggc cgcagccgga gagacccgca aaagccagcg
    14521 aagccgccat cagcttcaga aaatcgcggc ggtcgacgga gtcgtcccat tcggaaggcg
    14581 cctggcgcgg aaattcacga tgcagcaact cttcgaattc tggatcaccg gcgagttcct
    14641 caagggtacg ccagtactgc ttgccggttt tcgattggag cttggcgcgt accgcggcca
    14701 aatccatcgg gcttgtgtgc agcttcgcct ctgcggccgg atcattgtga tcgtggtggc
    14761 cgtcgaggtg ttcgtgttgt tcgtgctcgc gcataccgtc cattagcgat gacaggtcga
    14821 gcagctcgtg agctcggccg ttgtccgaat gttgtactca accttcagct tggcgccgat
    14881 ttgcacctga tcgtccggag ccttccaatc catattgaaa acctgatctt tcgggcggag
    14941 aacctgctcg ggattgcggt ggcagtccag gcaccattgc atcagcaacg tgttggcctg
    15001 gaataccagc ggcatctggc tgatctgccc gtggcacgac gagcagccca cgcccttatt
    15061 gacatgaata ctgtgatcga aatagacgaa atcggcgagg cggtggactt tgacccactg
    15121 gatggatttg tcactgcgat agctctcgcg aatgggctcg aggtaggccg cattattgaa
    15181 gagcaccgaa tggcagttca tgcaggtctt cgtcggcggg atgccagcaa cggcggaagt
    15241 ttccacgccg gtatgacagt agcggcagtc gatgccgtca tcgccgacgt gatgcttgtg
    15301 gctgaattgc accggctgct cacgggaaac gttctgccgc gtcatgtagg gcgagcgcgc
    15361 cacc
//
LOCUS       DASYVO010000049         1961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_44107, whole genome shotgun sequence.
ACCESSION   DASYVO010000049 DASYVO010000000
VERSION     DASYVO010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44107"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..1151
                     /locus_tag="VGR03_01830"
     CDS             279..1151
                     /locus_tag="VGR03_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEV1993045.1"
                     /translation="MMGVDVTLLSAFVAGLVSFLSPCVLPLVPGYISMLSGVGVEQLR
                     KGEGAKGILFSSALAFVTGFSVVFISLGASASSVGQFLRHNKSSLAPVAGAMILLFGL
                     HLIGWLAKIPISVGAAIGVVLVSAGLILMRAPGALAGKVEPIQFFALSVIFLFGPLLS
                     QWLSRDVHFRNLGGQPGAVGGFLMGFAFAFGWTPCIGPIFAAVLLVAASRETIWQGIF
                     LLAAYSAGLAVPFLLTALGVNRFLGFYQRFRRHLHTVEVLSGVLLLFVGALVFTNRFT
                     WLSGKLAFLNRFML"
     gene            1164..1745
                     /locus_tag="VGR03_01835"
     CDS             1164..1745
                     /locus_tag="VGR03_01835"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV1993046.1"
                     /translation="MKKTSFLVIAVALLVAVTLVVQRTRRISAAPRLAASGAAASAEA
                     KEDAPNFTVKDLNGASVSMTDYKGKVVLINFWATWCEPCRVDIPWLIGFQNKYAARGF
                     TVLGMAMDDGGVKDVDPFVRDERFDVDGQKLPMNYPILIGNDDVGDKFGGLLGLPTSV
                     LISRDGKKIKTFIGLVDHEKFVKAIEEQLQTSN"
     gene            1742..>1961
                     /locus_tag="VGR03_01840"
     CDS             1742..>1961
                     /locus_tag="VGR03_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="frataxin domain-containing protein"
                     /protein_id="HEV1993047.1"
                     /translation="MIDEHDFRKKSDAALETLRKRLLELGDQLGFEVEGQGEKLEILF
                     EEPEEVRFVVSPNVAARQIWISALSTSFK"
BASE COUNT          348 a          591 c          589 g          433 t
ORIGIN      
        1 ccgattggcg tacagatgat cggacgcgcg tttgacgagg cgagcctgct gcaactggcc
       61 gcggcctatc aacggcgaac cgactggcac ttgcggcggc ctcccctcgg ctgaatctgt
      121 gggaagatag tattcaggat caacaagaag gaccgcatag caaaggatca gcaagaagga
      181 ccgcataact aaagactgcg aagcctgcag ccgggttcgc gcatccaatt taacgcggcg
      241 agtttgccgc cccggctgcg catccggaca acaggagaat gatgggcgtc gacgtaacat
      301 tgctgagcgc atttgttgcc ggcctggttt cgttcctctc gccgtgcgtt ctgcctctcg
      361 tccctggtta catctccatg ctttccggcg tgggcgtgga gcagttgcgc aagggcgagg
      421 gcgcgaaagg aatcctgttc tcctcggcgc tggcatttgt cacggggttt tccgtggtgt
      481 tcatctctct gggcgcctcc gccagcagtg tcggccaatt tctgcgccac aacaaatcgt
      541 cgctcgcgcc cgtggccggc gcgatgatcc tgttgttcgg cctgcacctg atcggctggc
      601 tcgcgaagat tcccatttcc gtgggtgcgg ccattggtgt cgtgctggtg tccgcgggtc
      661 tcattctgat gcgcgcaccc ggagcgctcg cgggcaaggt cgagccgata caattcttcg
      721 ccctttcggt gatttttctc ttcggcccgt tgctttcgca gtggctcagc cgcgacgtcc
      781 atttccgaaa tctcggcggg cagcccggcg ccgtcggcgg ctttctgatg ggctttgcgt
      841 tcgctttcgg ctggacgccc tgcatcggtc cgattttcgc ggccgttctc ctggtggcgg
      901 cgtcccgcga aaccatctgg cagggaattt ttctgctcgc agcctactcg gcggggctgg
      961 ccgtgccctt tctgctcacc gcgctcggcg tcaaccggtt tctcggtttc taccagcgat
     1021 tccgcaggca cctgcacacc gtggaagtgc tgagcggcgt tctgttgctg ttcgtcggtg
     1081 cgctggtttt cacgaatcgc tttacctggt tatcgggtaa actagccttt ctcaatcgct
     1141 tcatgctcta gggatcgact gccatgaaaa agacaagttt tctcgtcatt gctgtggcgc
     1201 tgctggtggc cgtgaccctc gtcgtccagc ggacgcggag aatctcggcc gctccccggc
     1261 tcgccgcctc tggggccgca gccagcgcgg aagccaaaga ggatgcgccg aacttcacgg
     1321 tgaaggatct gaacggcgca agcgtcagca tgaccgacta caagggcaaa gttgtgctga
     1381 tcaatttctg ggccacgtgg tgcgagccat gtcgcgtgga cattccgtgg ctgatcgggt
     1441 tccagaacaa gtatgccgcc cgcggcttca ccgttctcgg catggccatg gatgacggcg
     1501 gggtgaaaga cgtcgatccc tttgtgcgcg acgagcgctt cgacgtggat gggcagaagc
     1561 tccccatgaa ttatccgatc ctcatcggca atgacgacgt gggggacaaa ttcggcggct
     1621 tgctcggcct tcccacctcc gtgctcatct cccgcgatgg caagaagatc aaaaccttca
     1681 tcggcctggt ggatcacgag aagtttgtca aggcaatcga agagcagctc caaacatcca
     1741 attgatcgac gaacacgatt tccgcaagaa atcggatgcc gcgctcgaaa ctctgcgtaa
     1801 acgcctcctc gagctcggcg accaactcgg cttcgaagtc gagggccagg gcgaaaaact
     1861 ggaaatcctt ttcgaggagc cggaagaggt acggttcgtc gtgagcccca acgtcgcggc
     1921 acgccagatc tggatctccg cgctatccac cagcttcaag c
//
LOCUS       DASYVO010000050         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_44144, whole genome shotgun sequence.
ACCESSION   DASYVO010000050 DASYVO010000000
VERSION     DASYVO010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44144"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..>2447)
                     /gene="mfd"
                     /locus_tag="VGR03_01845"
     CDS             complement(105..>2447)
                     /gene="mfd"
                     /locus_tag="VGR03_01845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV1993048.1"
                     /translation="ETITCVVPTTGKADRLRELLVEYEIPFETADAIKNAAPQAGTPG
                     GNDADGTSAPSVPFDLRTRVPIIIARGEMEEGVAFPDLRSLFLSDNDLFGGFVWGGHG
                     RKERSGVSSFLSDLSDLKVGDYVVHVDHGIGLYQGLRQLGVEGAPRDFMLLTFQDDAR
                     LYVPLERLDLIEKYRRTSEAGKPALDRLGGATWARTKTRVKRSLRDMAAELLRLYAER
                     KMRGGVAYSPDSTWQREFEDAFAFEETPDQLTAISEIKKDLESSQPMDRLLCGDVGYG
                     KTELAMRAAFKVAQDGKQVALLVPTTVLAFQHFTTFSQRMASFPVRVAMLSRFRSPAE
                     QKQIAVDAEAGKVDILIGTHRMLSKDVRFRDLGLLIVDEEQRFGVAAKEKLKTFAAGV
                     DVLTMSATPIPRTLHMSIGGLRDLSVIETPPRGRLAIQTVVAPFTDGVIQAAIMHEME
                     RAGQVFFVHNRVESIFSMAAIIQRLVPTARIGVAHGQMGEKELERAMLKFIQGEYEVL
                     VATALIENGLDIPRANTIIVNHADRFGLADLYQLRGRVGRSNRRAYAYFLIPAEDALT
                     PLAKRRLAALKEFSDLGAGFRLAALDLELRGAGNLLGGEQSGHLNSVGLDLYLQMLED
                     TVEELKGAPAKLEVRTTLNLGLDIKIPEQYIVDESQRLRMYKRISSLTEPEARAEIEA
                     ELVDRYGPIPTSVVNLLNYAVLKSVAEGLLVQSIDRKLDEVWIRFHPQAPVSPDKLTQ
                     FVRRNRGASFRPDGALRFRFTAPELSLPEQLANTLQQLHQ"
BASE COUNT          505 a          727 c          714 g          501 t
ORIGIN      
        1 ttcaaaccta ccttgatgaa aatctattgc gagcgaggga atattgtgtt cctgtgcaaa
       61 ggctggacgc ctagtcagcg tcatcttcct ctttcatctt agtgctactg atgtagttgc
      121 tgcaaggtgt ttgcgagttg ctcgggcaag gacaattccg gcgcggtaaa acgaaagcgc
      181 aaagcgccgt cgggccgaaa gcttgcgccg cgattacggc gcacgaattg ggttagcttg
      241 tccgggctta ccggggcttg cggatggaag cgaatccaca cctcgtcaag cttcctgtca
      301 atcgactgga ccagtaggcc ctccgctacc gacttcaaga cggcatagtt caggagattc
      361 acgacggaag taggaatcgg tccgtagcga tccacgagct cggcctcaat ctccgcccgc
      421 gcctcaggct cggtgagact ggaaatccgt ttatacatcc gcaagcgttg actctcgtcc
      481 acgatgtact gttcgggaat tttgatgtcg agtcccaagt tgagcgtggt gcgaacttcg
      541 agcttggcag gcgcgccctt cagttcttcc acggtgtctt ccagcatttg gagatagaga
      601 tcaagcccca cggaattcag atgcccggac tgttccccgc ccagtaaatt cccggcgccg
      661 cgtagttcca ggtccaacgc cgcgagacgg aagccggcac ccagatcgga aaattccttg
      721 agcgcggcca agcggcgctt ggctaaagga gtcagcgcgt cttcagccgg aatcaggaaa
      781 taagcgtagg cgcgccggtt cgagcggccc acgcggccgc gcaattgata aagatcagcc
      841 agcccaaagc ggtccgcgtg attgacgatg atcgtgttgg cccggggaat gtcgagcccg
      901 ttctcgatga gagcggtggc gaccagcacc tcgtattcgc cttgaataaa cttaagcatc
      961 gcacgctcga gctccttctc ccccatctgg ccatgagcga cgccaatgcg cgccgtggga
     1021 accaaacgct ggatgatcgc cgccatgcta aagatagatt ccacccgatt gtgcacgaag
     1081 aagacctgcc cggcacgttc catttcatgc attatcgcgg cctgtatgac gccatcagtg
     1141 aaaggcgcaa ccacggtttg gatggcgagg cggccgcgcg gcggcgtttc gatcaccgaa
     1201 aggtcgcgca agccgccgat ggacatgtgc aaggtgcggg gaatgggagt ggccgacatg
     1261 gttaacacat cgacaccggc ggcaaatgtt tttagcttct ctttggcggc gactccgaag
     1321 cgctgttctt cgtccacgat gagcaagccg aggtcacgaa agcgcacgtc tttcgaaagc
     1381 atgcggtgcg tgccgataag gatatcaacc ttaccggctt cggcatccac ggcgatctgt
     1441 ttttgttctg ccggcgagcg gaaacggctg agcatcgcca cgcgtaccgg gaacgacgcc
     1501 atccgctggc tgaaagtagt gaagtgttgg aacgccagga cggtggtggg caccagcagc
     1561 gccacttgct ttccgtcttg cgccacctta aacgcggcgc gcatcgcaag ctcggtcttg
     1621 ccgtacccta cgtcaccaca aagcaggcga tccatcggct gcgagctttc caggtccttt
     1681 ttaatctcgc tgatggcggt taactggtcc ggcgtttcct caaacgcaaa cgcgtcttcg
     1741 aactctcgct gccaggtgga atccgggctg taagccacgc cgccccgcat cttgcgctcg
     1801 gcatagaggc gaaggagctc ggcagccata tcgcgcagag aacgcttcac ccgcgttttg
     1861 gtgcgggccc aggtggcgcc gccgaggcga tcgagtgcgg gcttgcccgc ttcgctggtg
     1921 cgccgatact tttcgattag gtctaatcgt tcgagcggca catacaaacg ggcgtcgtcc
     1981 tggaaggtga gcagcatgaa gtcgcggggc gcgccctcca cacccagttg ccgcaagccc
     2041 tggtagaggc cgatgccgtg atccacgtgc acgacgtagt cgcccacttt cagatcgcta
     2101 aggtcggaaa gaaaactcga aaccccggag cgttcttttc gcccgtgccc tccccaaacg
     2161 aatccgccaa acaagtcatt gtcggaaaga aacagtgatc ggagatcggg gaacgctact
     2221 ccctcttcca tctcccctcg cgcgatgatg atgggtacgc gcgttcgcag atcgaacggc
     2281 acagacggcg ctgatgttcc atcagcatcg tttccacccg gcgttcccgc ctgcggcgcg
     2341 gcattcttaa tcgcgtcggc ggtttcaaaa gggatttcgt attccaccag cagttcgcgc
     2401 aggcggtcag ccttgccggt ggtgggaacc acgcacgtaa tcgtctc
//
LOCUS       DASYVO010000051         3938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_71378, whole genome shotgun sequence.
ACCESSION   DASYVO010000051 DASYVO010000000
VERSION     DASYVO010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71378"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VGR03_01850"
     CDS             complement(<1..322)
                     /locus_tag="VGR03_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEV1993049.1"
                     /translation="MKSSYNGKAVPSEGKAIGYSGGELQVPDTPIIPFIEGDGTGRDI
                     WKASRRVFDAAVEQAYGSKRRVAWFEVFAGQKAFKTFNEWLPNDTVDAIRDFRVAIKG
                     PLTTP"
     gene            842..2635
                     /locus_tag="VGR03_01855"
     CDS             842..2635
                     /locus_tag="VGR03_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEV1993050.1"
                     /translation="MQQTFAVGIGGAAGQGVATPGDIFAKIFSRRGLNLNAYNAYQSI
                     IRGGHTFLTIRAGPGKVTNMGDRTDLLIPLNQDSMDRHLKLLSAGAACIYNADTIKPG
                     SAAEGVQLCPLPVSELADITRNKVAQNTVAIGAGLHMMGIGFQALESVLQEQFKKKGE
                     AMVAENVGVARAGYDYAAVRFTAFPNPLPKTENRYAILSGNTAMAMGGAAAGVKFYCA
                     YPMSPATGVLHWMAAHARKAGIMVRQVEDEIGVINMAIGAAHAGVRAMCATSGGGFAL
                     MSEGLGMSAMMETPVVVINCQRAGPSTGVPTKTEQGDLWQMLGAAFGDYPRVIAAPLD
                     IGDCFKLIPEIFNIADRFQCPGLVLCDLLLSEGRLSVHPKDLDFSPSIDRGELITSSN
                     GGSPGVATNGDYKRYKITESGISPRAVPGVPGHIHTVATDEHMEDGVLLSDEFTNPIK
                     RRAMMEKRMRKVTGIDAAVPPPVLMGPRDADVTLIGWGSTKGVIEESCEILNEQGISA
                     NQLQIRWLVPLHGDAILEILKGSRHTIMVENNYSGQFARYLRSETSFVPDGHIRKYDG
                     EPFMPHHIVEAVKEQLAGKTKLSVPMHEIMV"
     gene            2649..3545
                     /locus_tag="VGR03_01860"
     CDS             2649..3545
                     /locus_tag="VGR03_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEV1993051.1"
                     /translation="MATGVQHASQLTMADMKGRVDPDWCPGCGDFGVLAAVQKALVEL
                     QIPNYKVVTISGIGCSSNFPGYMNTYGMHTLHGRALAVATGLKLANHELTVVVTGGDG
                     DGFGIGGNHFVHSMRRNVDLLYIVMDNQIYGLTTGQTSPTSRIGMKTKSMPFGNIEAP
                     VNPISLALAAGATFIARGYSAEQKHLTELIKQGIEHPGFSFLDVFSPCVTYNHDNTYQ
                     WFRPRVKKLEDDSTYDPLNWMAAIEKSLLWGDEIPIGKFFERNDVPTLHGAEPVLNEG
                     PLVHRDLRVPLDAARSFVEELM"
     gene            3708..>3938
                     /locus_tag="VGR03_01865"
     CDS             3708..>3938
                     /locus_tag="VGR03_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV1993052.1"
                     /translation="MDWNQYHKEVGARVGDLAKLSPDTVRGYQTLSAANSKTSKLGEK
                     TRQLISLAVAVTTRCDGCIVFHTDAALKAGATK"
BASE COUNT          831 a         1181 c         1153 g          773 t
ORIGIN      
        1 ctggcgtggt gagcggtcct ttaatggcca ctcgaaaatc acgaatcgcg tcgaccgtat
       61 cgttcggcag ccattcgttg aacgtcttga aagccttctg gccggcaaac acctcgaacc
      121 acgccacgcg ccgcttcgag ccgtaggcct gctccaccgc cgcgtcgaaa acgcgccgcg
      181 acgctttcca gatgtcacgc cccgttccat ccccctcgat gaacggaata atcggcgtgt
      241 ctggcacttg cagctcgccg ccgctgtaac cgatcgcctt gccttcggag ggaacagcct
      301 tcccgttata cgaactcttc atgaatacgg ctccttggaa taagttcgac tgctctaccg
      361 gcgcacgagg aaagggatgc gccgcgccgc aacaacagcc tcgcaaccca gcaggctcgg
      421 gtgaactgga gaagatatca tactcacgca gccggagagt ataatcgggc ccgatcggta
      481 gtggttcacc agttttcgga aagggcgttc gccaggcacg atcctgattg ctggtgcgaa
      541 gttctcttgt cgccggccag gcagccgatg ttttcattga tgaccccttc ctcggcgtga
      601 gttccgttgt tttgcatggg cacgggttgt atactcgggg ccatcctaag gagcaaattg
      661 gaaatttcgg tatgcagttt gtcgcacagg ccaggaaact tgacctgagt ccgtccagcg
      721 cggttgagag cactttggac cgcgcggatg agcccagaat gctagagccg atcgcgggcc
      781 gccagcagat ctgaaggttc agaagtctct gctgcgctgg tttccgcttg gggagggtgc
      841 tatgcagcag acctttgccg tcggtatcgg aggcgcggcc ggccagggtg tcgccacgcc
      901 gggcgacatt ttcgcaaaaa tcttcagtcg gcgtggcctt aacctgaatg cttacaacgc
      961 ctatcaatcc atcattcgcg gcggccacac ttttctgacc attcgtgcgg gcccgggaaa
     1021 ggtcaccaat atgggtgacc ggaccgacct gctcattccg ctcaaccagg acagcatgga
     1081 tcgccacctg aagctgctca gtgccggcgc tgcgtgtatc tataatgctg acacgatcaa
     1141 gccgggctcc gccgccgaag gcgtgcagct ctgcccgctt ccggtttccg agttggccga
     1201 catcacccga aataaggttg cgcagaacac cgtcgcgatc ggcgccggcc tccacatgat
     1261 gggtatcggc ttccaggcgc tcgagagcgt tctccaagag cagttcaaga aaaaaggcga
     1321 ggccatggtc gccgaaaatg tcggagttgc gcgtgccggc tatgattacg ccgccgtgcg
     1381 cttcacggct tttccgaatc cactccccaa gacggagaat cgctacgcga tcctgagtgg
     1441 caatactgcg atggccatgg gtggggcggc agcgggtgtg aaattctatt gtgcctaccc
     1501 catgagccct gcgaccggcg tgctgcactg gatggcggcg cacgcgcgca aagccggcat
     1561 tatggtccgg caggtggagg acgagattgg cgtgatcaac atggcgatcg gcgcggcgca
     1621 tgccggcgtc cgcgccatgt gcgctacctc gggcggcggc ttcgccctga tgagcgaggg
     1681 cctgggcatg tccgccatga tggaaacccc ggtggtggtt atcaactgtc agcgagccgg
     1741 cccgtccacg ggcgttccga cgaagaccga gcagggcgac ctctggcaga tgctcggcgc
     1801 cgcctttggc gactatcccc gagtgatcgc ggctccgctg gacatcggcg attgcttcaa
     1861 gctgattccc gaaatattca acatcgccga ccgcttccag tgcccgggtc tggtgctttg
     1921 cgatttgctg ctgtcggaag gcaggctcag cgtacatccg aaagatctgg atttcagccc
     1981 ctccatcgac cgcggcgagc tcatcaccag cagcaacggt gggtcccccg gcgttgccac
     2041 gaacggggat tacaaacgct acaaaatcac cgagagcggc atttcaccgc gcgctgttcc
     2101 cggcgtgccc ggccacatcc acacggttgc caccgacgaa cacatggaag acggcgtatt
     2161 gctaagcgac gaattcacca atccgatcaa gcgacgcgcg atgatggaaa agcgcatgcg
     2221 caaggtgacg ggcattgacg ccgccgttcc gcctcccgtg ctgatgggtc cgcgggacgc
     2281 cgatgttacg ttgatcggct ggggttcaac gaaaggtgtg atcgaggaat cctgcgagat
     2341 cctcaacgag caaggaatct ctgccaatca gctgcagatc cgctggctgg tgccgttgca
     2401 tggcgatgca attctcgaaa ttctgaaagg ctcgcggcac accatcatgg tggagaacaa
     2461 ctacagcggc cagtttgcgc gctacctgcg cagcgaaacc agctttgtgc cagacggcca
     2521 cattcgcaag tacgacggcg aacccttcat gccgcaccac attgtggaag cagtgaagga
     2581 acagctcgcc ggaaagacga aactctccgt tcccatgcac gagattatgg tctgaggagg
     2641 agacgcatat ggcgacaggc gtacagcatg ccagtcaact gacgatggcc gatatgaaag
     2701 gcagagtcga tcccgactgg tgccccggct gcggcgattt cggcgtgctg gcggcggtac
     2761 aaaaagccct ggtcgagctg cagataccga actataaggt ggtaaccatc agcggcatcg
     2821 gctgttcctc caacttcccc ggctacatga atacctatgg catgcacacc ctgcacggcc
     2881 gggccctggc ggtggccact ggtctgaagc tcgccaatca cgagctaaca gtagtggtga
     2941 cgggtgggga cggtgacggt ttcgggatcg gcgggaacca ttttgtccac tcgatgcgcc
     3001 gcaacgttga cctgctctac atcgtcatgg acaaccagat ctacggcctt accacgggcc
     3061 agacttcgcc gaccagccgc atcggcatga aaacgaaaag tatgccgttc ggcaacatcg
     3121 aagcgccggt taatcccatt tctctggctc tcgccgcagg cgccaccttc atcgcccgcg
     3181 gctacagcgc tgagcagaag cacctcacgg aactgatcaa gcaaggtatt gagcacccgg
     3241 ggttctcgtt cctggacgtc ttcagcccgt gcgtcacgta taatcatgac aatacctacc
     3301 agtggttccg tccgcgcgtg aaaaagctcg aagacgactc cacctatgac cccctcaatt
     3361 ggatggccgc cattgaaaaa tcactgctct ggggggacga gattcccatc gggaaattct
     3421 tcgagcgcaa cgacgtgccg actttgcacg gtgccgagcc ggtcctgaac gaaggcccgc
     3481 tggttcatcg cgacctgcgg gtgcctctcg acgcggcccg ctcattcgtt gaagagttga
     3541 tgtagtgacg gggggcagcg cttgcgacca aacccgtgtg attcattttc cagccgtgca
     3601 cgcatgggta ggggtgtgta agattggtct cgacaaatcg ctttcccgga aactgacctg
     3661 ctatggactg attccggctt cacaatatag gagagaaaaa catgatcatg gattggaatc
     3721 aataccataa ggaagtcggc gcccgagtcg gtgacctggc gaaactttct ccggacaccg
     3781 ttcgcggtta tcagactctt agtgccgcca attcaaagac cagcaaactg ggcgaaaaaa
     3841 cgcggcagct catctcgctg gccgtcgctg tcacaacacg ctgcgacggc tgcatcgtct
     3901 tccatacgga cgccgccttg aaggctggag caaccaaa
//
LOCUS       DASYVO010000052         3971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_226502, whole genome shotgun sequence.
ACCESSION   DASYVO010000052 DASYVO010000000
VERSION     DASYVO010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226502"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1001
                     /locus_tag="VGR03_01870"
     CDS             126..1001
                     /locus_tag="VGR03_01870"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV1993053.1"
                     /translation="MTWKRVATAAVLIPFVVGIVLWCSTAIVSLCIALITILALFEYF
                     ALGEAIGHRTYRFWTATCALILVFGQWRAAFASTYSLPGGLTAYRKTEWFAGGMPSAE
                     VALFLFVLGIAASTLFTKRPMVEALPAAGISSSGLILVAFPLSFAIRIHGSGMQGPLL
                     LLFAMVIIWVGDTAAYFVGRSIGKHALAPHLSPKKTWEGTVASFLGSLIVALIFARYM
                     TVPLPHLLAMAALGNVAGQVGDLLESAYKRSAGIKDSGSLLPGHGGVLDRIDALILAI
                     PVVWYYWILVYSPRA"
     gene            1031..2317
                     /locus_tag="VGR03_01875"
     CDS             1031..2317
                     /locus_tag="VGR03_01875"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEV1993054.1"
                     /translation="MTEKRRQERQIQRKKSGGKPPHSKRESNDSVKQLAILGSTGSIG
                     RQCLSVLEALPESFGVVALAAGANLEELIGQIERHRPEVVSVGDAQRADELSRSLREK
                     GISQLPEIHHGREGMLAVGTHSKADIVVSAAVGVVGLEATYEAVKLGKTVALSNKEVL
                     VAAGELVMAAAKKAGRGLLPVDSEHNAVHQCLRGGTHGEVRRLVLTASGGPFRKTPLA
                     ALANVTTEQALAHPNWRMGNRITIDSATMMNKGFEVIEARWLFGMRPDQIDVVVHPQS
                     TIHSMVEFVDGSVLAQLGPTDMRMPIQYALTYPERVASNQVALDWSKLKRLDFAKAST
                     RRFPCLRLAREAMKKGGALPCALNAADEIAVAAFLERRLPFLGIPEVIERVLGRTPRV
                     RFEKMEDVLTADAEARRMAREEVERLAVTAAAASTR"
     gene            2333..3667
                     /gene="rseP"
                     /locus_tag="VGR03_01880"
     CDS             2333..3667
                     /gene="rseP"
                     /locus_tag="VGR03_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV1993055.1"
                     /translation="MNLLLGVALVLGLMIMIHEWGHFIVARLFKVRVDVFSIGFGPRL
                     FGWKRGATDYRVSALPFGGYVRMAGQDLSEVDSNDVAPTGAPDELMSKPRWQRALISF
                     AGPAVNLIFPLLLLTALFVAVGLPYSSFYDLPVQVVALPADKAGQPGRLQVGDKLLSI
                     NDVRNPTWEQAQKSLKQAAPGDKLKLEVENAGNKRNVEFALASGTTLDRIVGYPPLPP
                     ILDEVAPGTPAQRAGLKEGDRIRAVDGQKIDYWGQFVERVRGSKGQGVKLDLERKGQA
                     IHADVTPQASVAESGETIYQVGVAVLDPTSYRRVSFSDGVRDASVMTGERIQETIGVV
                     GKLFSGRVSVRQLQGAVGISRAAGEAVRKGPLAVISLMVLISVNLGILNLLPIPILDG
                     GNILLLTLEGIRRRDFSLAFKERFVQVGFVFLLVLFAYVMYNDVVRMLPTHS"
     gene            3700..>3971
                     /locus_tag="VGR03_01885"
     CDS             3700..>3971
                     /locus_tag="VGR03_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEV1993056.1"
                     /translation="MAASPSHRVSTGERLNTAETTGKIQQAVAARGIRMTRQRRVILQ
                     VMDDAEQHLDVDQILARAQKIDAGVHLVTVYRTIDLLKKQGLIDEL"
BASE COUNT          761 a         1064 c         1278 g          868 t
ORIGIN      
        1 gtaactcacg ccttgcagcc cggcacgcgt cgcgtaggta aatgcctgtc ctagttctcg
       61 tgcgtgattt atttctctca atgtgatgag atgaaggcgg cggttactca ggggattcca
      121 tttctatgac ctggaagcgg gtggcgacag ccgcggtgct gattcctttt gttgtgggca
      181 ttgtgttgtg gtgctcgacg gcgatcgttt cgctctgcat tgcgctgatc acgattcttg
      241 cgctcttcga atatttcgct ttgggcgagg ctatcgggca tcgcacgtac cgcttctgga
      301 cggcgacttg cgctctgatc ctggtctttg ggcaatggcg tgctgcattt gcatcgactt
      361 attcactgcc agggggttta accgcctatc gaaagacgga gtggtttgcc ggaggaatgc
      421 cttcggccga ggtggccttg tttttgttcg ttctcggaat tgcggcaagc accctattta
      481 caaagcgtcc aatggtcgaa gcgcttcccg cggcagggat cagttctagc ggtctcattc
      541 tggtcgcgtt tccactgtcc tttgccatcc ggattcacgg ttcgggaatg caggggccgc
      601 tgctcctgct cttcgctatg gtcatcatct gggtgggaga tactgcggct tacttcgtcg
      661 ggcgatccat tgggaaacat gctctggcgc cgcatctgag cccaaagaaa acgtgggagg
      721 gaacggtcgc gagtttcctt ggttcactga tcgtggctct gatttttgcg cgttacatga
      781 ctgtgccgct gccgcatctt ctcgcgatgg ctgcacttgg caacgtggcg gggcaagttg
      841 gcgaccttct ggaatcggct tacaagcgga gcgcggggat caaggattcc gggagcttgc
      901 tgccgggtca tggcggagtc cttgaccgaa tcgacgcgct catcctcgct attccggttg
      961 tctggtatta ttggatcttg gtctactctc ccagggcgta gctcagcggg cttcctgccg
     1021 gcaagcgtgg atgacggaaa aacggcggca ggaacggcaa atccaaagga agaaaagcgg
     1081 cggcaagccg ccgcactcca aaagggaatc gaacgactcc gtgaaacagt tagccatttt
     1141 gggttcgacc ggttcgatag ggcggcagtg cctttcggtc ttggaggctc tgccggagag
     1201 tttcggtgtc gtcgcgctgg cggcgggagc caacctggaa gagctgatcg ggcagatcga
     1261 gcggcaccgg ccggaggttg tctccgttgg agatgctcag cgggccgatg agttgtcgcg
     1321 gtcgctgcgc gaaaagggaa tttcgcagtt gccggagatt caccacgggc gcgaaggaat
     1381 gttggcggtt gggacgcatt cgaaagcgga catcgtggta tcggcagcgg tgggcgtcgt
     1441 ggggctggaa gcgacgtacg aagcggtaaa gctgggaaag acagttgcgc tgtcgaacaa
     1501 agaagttctg gtcgcggcgg gcgagctggt catggctgcg gccaaaaaag ccgggcgcgg
     1561 acttcttccc gtcgatagcg aacacaacgc cgtgcatcaa tgcctgcgcg ggggaacaca
     1621 cggagaagtg cgacggctgg tattgacggc atcgggcggt ccgtttcgaa agacgccgct
     1681 ggccgcgctg gcaaatgtca ccaccgaaca agcgctggcc catcccaact ggcgcatggg
     1741 aaatcgcatc accatcgatt ccgcgacgat gatgaacaaa ggctttgagg tcatcgaagc
     1801 gcgctggctt ttcggtatgc gcccggacca aattgacgta gtcgtgcatc cccagtccac
     1861 gattcactcg atggtcgagt tcgtggacgg ttccgtttta gcgcaactgg ggccaacgga
     1921 catgcgcatg cccatccaat atgcgttaac ctatccggag agggtggcat ctaatcaggt
     1981 ggccctggat tggtcgaagc tgaagcgctt ggattttgcg aaagcttcga cgcggcggtt
     2041 cccctgcctg cggttggcgc gggaagcgat gaagaagggc ggcgcgctgc cgtgcgcgtt
     2101 gaatgccgcg gatgagatcg ccgtggcagc gtttctcgaa cggcggcttc cgttcctggg
     2161 tatcccggaa gtgattgagc gcgtgctcgg gcggacacca cgcgtgcgat ttgagaagat
     2221 ggaggacgtg cttacggcgg atgccgaagc gcgtcgaatg gcaagggaag aagtagagcg
     2281 gctggcagtc acggccgcgg cggcgtcaac aaggtaagga gaacggaatc gtatgaattt
     2341 gttactgggc gtcgccctcg tactgggcct gatgatcatg atccatgagt ggggccactt
     2401 catcgtggcg cgtttgttta aagtgcgcgt ggatgtcttc tcgattgggt ttggtccgcg
     2461 cctgtttggt tggaaacgcg gcgcgacgga ctaccgcgtg agcgcgctgc catttggcgg
     2521 ctacgtacgc atggccggac aagatttatc cgaagtcgat tccaatgacg tagctcccac
     2581 tggcgccccc gacgaattga tgagcaagcc gcggtggcag cgagcgctga tttcttttgc
     2641 cggaccggcc gtcaatctga ttttcccgct tcttctgctt acggcattgt ttgtcgccgt
     2701 gggcctgcct tactcgtcct tctacgatct gcccgtgcag gtcgtcgcgt tacccgcaga
     2761 caaggccgga caaccgggcc gtttgcaagt gggcgacaaa ctcctttcca tcaatgacgt
     2821 ccggaatcct acctgggagc aggcccagaa atcgctgaaa caagctgctc cgggcgacaa
     2881 actaaaattg gaagtcgaaa acgcgggtaa caagcgcaac gtggaatttg ctctggcaag
     2941 cggcacgacg cttgatcgca tcgttggcta tccgccactg ccgcctatcc tcgacgaagt
     3001 cgctcctgga acaccggcgc agcgcgctgg gctcaaagag ggcgaccgta tccgtgcggt
     3061 ggatgggcag aagatcgatt actggggaca gttcgtcgag cgcgtcaggg gctcgaaggg
     3121 ccagggcgtc aagctggacc tggaacgcaa gggccaagcg attcatgccg acgtgacgcc
     3181 tcaagcaagc gtcgcggaat ccggggaaac catttaccag gtgggagtgg ccgtgcttga
     3241 cccgacttcc tatcgccgcg tttccttctc ggatggcgtt cgtgacgcgt ctgtgatgac
     3301 cggcgaacgc atccaggaga ccattggggt cgtgggaaaa ctcttcagcg ggcgtgtttc
     3361 cgtcaggcag ctgcagggcg cggtaggcat ttcgcgcgct gcgggagaag ccgtgcgaaa
     3421 gggaccgctg gcggtgattt cgttgatggt tctaatcagc gtgaacctgg gaatcctgaa
     3481 tctgctgccc atcccgatcc ttgatggcgg aaacatcctt ttgctcacgc tggaagggat
     3541 tcgccgccgc gatttcagcc tagccttcaa ggaacgcttc gtgcaggtcg gattcgtgtt
     3601 cctgttggtc ctcttcgcgt acgttatgta caatgacgtg gtgcggatgc ttccgaccca
     3661 ttcctgagcc gcgactccgg cacgagcgga gaactacaaa tggctgcttc accctcccac
     3721 cgggtttcca ccggtgagcg cctcaatacc gccgagacaa ctggcaagat tcagcaggct
     3781 gtggctgccc gtggcatccg catgacccgg cagcgccggg tcatcctgca agtgatggat
     3841 gatgccgagc agcatctgga cgtggatcag atcctggcgc gagcgcagaa aatcgatgcg
     3901 ggagtgcacc tagtcacggt ttaccgcacg attgatctcc tcaaaaaaca aggattgatt
     3961 gacgagctgg a
//
LOCUS       DASYVO010000053         6614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_71405, whole genome shotgun sequence.
ACCESSION   DASYVO010000053 DASYVO010000000
VERSION     DASYVO010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71405"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2552)
                     /gene="aceE"
                     /locus_tag="VGR03_01890"
     CDS             complement(<1..2552)
                     /gene="aceE"
                     /locus_tag="VGR03_01890"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEV1993057.1"
                     /translation="MENEISTQPHGMEDVEIQEWLESLDSVLESSGPEVAAEILERLR
                     AHATVNGIDLPFSANTPYANTIPVRLQPLFPGDQELERRIKSLIRWNALAMVVRANRV
                     EHNIGGHISTYASAATLYEVGFNHFFRARTEEFEGDTVYFQGHASPGMYARAFLEGRL
                     SAQKLENFRRELKPGGGLSSYPHPWLMPDFWEFPTVSMGLSPLMAIYQARFNRYLEDR
                     GMKPVTDAKVWAFLGDGETDEPESLGAITLASREKLDNLIFVINCNLQRLDGPVRGNG
                     QIIQELESAFRGARWNVIKVLWGSEWDPIFERDTEGLLVERMGKTVDGEYQKLVVSSG
                     TYVREHFFGQDERLLKLVEPMSDESLRRLRLGGHDPRKVYAAYKAAVEHKGSPTVILA
                     RTIKGYGLGEAGEGRNITHQQKKLNEDELREFRSRFGIPLNDEDCIEVPFYRPAEDSV
                     EIQYLRARREALGGYVPKRSIRTKPLVADHDELFKEFLEGSDGREVSTTMAFVGMLRK
                     MLKDAEIGKLIVPIVPDEARTFGMESLFRTAGIYSSVGQKYEPVDVNTLLYYKEAKDG
                     QILEEGITEAGSMASFIAAGSAYATHGINTIPFFIYYSMFGFQRIADFIWAAADMRTR
                     GFLLGGTSGRTTLSGEGLQHQDGNSQVLALPVPNIKAYDPAFAYEIAVIIQDGIRRMY
                     KDCENVFYYITVMNEPYAMPPMPGNVKDGILRGMYRFRSATNAKAKLRAQLFGSGAIL
                     NEALRAQEILETKYGVAADVWSVTSYKELYNDGNETERWNRLHPGEKARVPYVSKCLA
                     DAPGVLVAASDYLKTLPNMISKWMPRRLASLGTDGFGRSEGRASLRDFFEVDS"
     gene            complement(2732..3463)
                     /locus_tag="VGR03_01895"
     CDS             complement(2732..3463)
                     /locus_tag="VGR03_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1993058.1"
                     /translation="MSGESLQGRVALVTGSAKRLGRAVALRLAEEGADVIIHYRSSTL
                     DAQSAVAEIEKLGPRGRAIAADLSSVADIKRLFDEAAKRFGRLDILVNSAANFLPASI
                     ISTTEEIWDASLDSNLKAPFFCAQAAAQLLRRTNGTIINFADTGGLLGWPGYIAHSVS
                     KAGVVMLTKVLAKALAPEVRVNAIAPGTITMPGDPPEWEEDFIKRAPLHRTGKPSDIG
                     DAVVFLAYSKFITGQVLVVDGGRTL"
     gene            3617..4333
                     /gene="bshB1"
                     /locus_tag="VGR03_01900"
     CDS             3617..4333
                     /gene="bshB1"
                     /locus_tag="VGR03_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEV1993059.1"
                     /translation="MNLDLLAIAAHPDDVELTCGGTLLKMAQRGYKTGILDLTSGEMG
                     TRGTPETRAKEAARAAKLLAVKWRGTLGVPDSDVQPSRQHKLRLAAVIRQLRPKTVIL
                     PYWEARHPDHYHASTLGYEGCFLAGLKQLPIEGEPFRPFKILYSISYADVRPTFVVDI
                     TAQYQQRRKAILAFGSQFRPKKSEKKSKVHLAIDRLEAEMNQLARHYGQMIGVDYGEP
                     FLQKELMKVEDVVKMEVRSI"
     gene            complement(4346..4558)
                     /locus_tag="VGR03_01905"
     CDS             complement(4346..4558)
                     /locus_tag="VGR03_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993060.1"
                     /translation="MSTSKIQIGQLWKKDGTKDIYLVTRLYSEALNTMVILRKSGAED
                     EAQTRVRVERAGAGQTIPGFSPAQDD"
     gene            4832..5959
                     /locus_tag="VGR03_01910"
     CDS             4832..5959
                     /locus_tag="VGR03_01910"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEV1993061.1"
                     /translation="MRDVLRHTLKLALVIALVAGSTFAAPQSQDPPSQDPAKTPPPPA
                     QQQTQQQSSPQPVQQQDTVHPKNSKEDVEAIGNRSVGKGMNLYSLEREISLGKSLAQE
                     VEKSSKLIDDPIVTEYVNRVGQNLVRNSDARVPFTIKVIDSDEVNAFALPGGFFYVNS
                     GLIIRAQEESELAGVMAHEISHVTARHGTKNATKGELMQLATIPLILLGPGGWAGYGI
                     YQGLNLAIPVTYLKFSRDAEREADYLGLQYMYKAGYDPNSYVTFFERIQADEKRRPGT
                     IPKVFSSHPPTPERIENTQKEIARILPARQEYIVTTSEFDSVKARLRNIMFSRKVTDN
                     APGKPTLRTKTEQSKKQPGSTDPNSTDDDRPTLKRRPDSQP"
BASE COUNT         1342 a         2070 c         1820 g         1382 t
ORIGIN      
        1 gagtcgacct cgaagaagtc gcgcaatgag gcgcggcctt cgctgcgccc gaatccatcg
       61 gtgccgagag acgccaggcg ccgcggcatc catttgctga tcatattggg caaggtcttc
      121 aagtaatcgg aagccgcgac cagcaccccg ggagcgtctg cgaggcattt ggagacgtag
      181 ggcacgcgcg ccttttcgcc cgggtgcaaa cggttccagc gctcggtctc gttaccgtcg
      241 ttatagagtt ccttgtagct ggtcacgctc cacacatcgg cggccacgcc gtacttggtc
      301 tcgagaatct cctgcgcccg taacgcttcg ttgagaattg cgccgcttcc aaacaattgc
      361 gcgcgcagct ttgctttcgc gtttgtcgcc gaccggaagc ggtacatccc ccgcagaatc
      421 ccgtccttca cgttacccgg catcggcggc atggcgtacg gttcattcat cacggtgatg
      481 tagtagaaga cgttttcgca gtctttgtac atgcggcgaa tgccatcctg gatgatcacc
      541 gcgatttcat aggcaaacgc gggatcgtag gcttttatgt tcggaaccgg cagcgcgagg
      601 acctggctat tgccgtcctg gtgctgtagg ccttcgcccg agagcgtcgt gcgtcccgac
      661 gtgccgccga gcaggaaacc gcgcgtgcgc atatcggccg ccgcccagat gaaatcggca
      721 atgcgctgga aaccgaacat cgagtagtag atgaagaacg gaatcgtgtt gatgccgtgc
      781 gtggcgtacg cggaccccgc ggcaataaac gatgccatcg agccggcttc cgtgatgcct
      841 tcttcgagaa tctgtccgtc ctttgcttcc ttgtaataaa gcagggtgtt tacgtcgacg
      901 ggctcatact tctggccaac gctggaataa atgcccgcgg tgcggaacag cgattccatg
      961 ccgaaggtgc gcgcttcgtc cggaacgatg ggcacgatga gcttgccgat ctccgcatcc
     1021 ttgagcatct tgcgaagcat gcccacgaag gccatggtcg tggaaacttc cctgccatcg
     1081 ctgccttcca ggaattcctt gaagagttcg tcgtggtcgg caaccagggg cttcgtacga
     1141 atgctgcgct tgggaacgta gccgcccagc gcttcgcgcc gcgcacgaag atactgaatt
     1201 tcgacactgt cctcggccgg ccggtaaaac ggaacttcga tgcaatcttc gtcgttcaga
     1261 ggaatgccga agcgcgaacg gaattcgcgc agctcgtctt cgttgagttt cttctgctgg
     1321 tgcgtgatgt tcctgccttc gcccgcttcg ccaaggccgt agcctttgat ggtgcgcgcg
     1381 agaatgacgg tcggagagcc cttgtgctcc acggccgctt tgtacgcggc atagacttta
     1441 cgcggatcgt gcccgccgag gcgcagccgc cgcaggctct cgtctgacat cggttcgacg
     1501 agtttcagca gccgctcgtc ttgcccaaag aagtgctcgc gaacgtaggt gccggaggaa
     1561 acgaccagct tctggtactc gccatcaacg gtcttcccca tgcgctcgac cagcagacct
     1621 tccgtatcgc gttcgaagat ggggtcccac tcgcttcccc ataacacctt tatgacgttc
     1681 cagcgggcgc cgcggaatgc cgattcgagt tcctgaataa tctgtccatt gccgcggacg
     1741 ggaccgtcaa ggcgttgcaa attgcagttg attacaaaaa tcagattgtc gagcttctcg
     1801 cgcgaagcca gcgtgatcgc tccgagcgat tccggttcgt cggtctcgcc gtcacccagg
     1861 aaggcccaca ccttggcgtc cgtgacaggc ttcatgccgc gatcctcgag ataacggttg
     1921 aaacgcgcct gataaatggc catcagcggg ctgagtccca tggaaaccgt cggaaattcc
     1981 cagaaatcag gcatcagcca cggatgcgga taagaagaaa gcccgccgcc gggcttcagc
     2041 tcgcggcgga aattctcgag cttctgcgcg ctgaggcggc cttccagaaa cgcgcgagcg
     2101 tacatcccgg gtgaggcatg cccttgaaaa taaaccgtgt cgccctcgaa ctcctcggtg
     2161 cgcgcgcgga aaaaatggtt gaagcccact tcgtagagcg tcgcggccga ggcgtacgtg
     2221 gaaatgtgac cgccgatgtt gtgctcgacg cggttcgcgc gcaccaccat ggccagcgcg
     2281 ttccagcgga tgaggctctt gatgcggcgc tccagttcct ggtcgcctgg aaaaaggggc
     2341 tgcaagcgca cgggaatcgt attcgcgtaa ggtgtattgg cgctgaacgg caggtcgatg
     2401 ccgttgaccg tggcgtgcgc gcgcagccgc tcgaggattt ccgcggccac ctcgggtccg
     2461 ctggattcca gaacggaatc cagcgactca agccattcct gaatctctac gtcttccatg
     2521 ccatgaggct gggtcgatat ttcgttttcc ataacttcgg tcggtccctc gtcttttaat
     2581 ttgccggatg tgccgcgcct ggcgcggcgg atttatgcaa gcgattagca gaatagaata
     2641 caccgcagag cgattctctg cacggccgga aggaagattt ctcgagcgat gttgaggggc
     2701 atcggtcgtt ggactcgcgt tctcccggcc atcacaacgt gcgcccgcca tccaccacga
     2761 gcacctgccc cgtaatgaat ttcgaataag ccaggaacac taccgcgtcg ccaatgtcgg
     2821 acggtttgcc ggtgcgatga agcggtgcgc gcttgatgaa gtcctcttcc cattcaggcg
     2881 ggtcgccggg catggtgatc gtgccgggcg cgatagcgtt gacgcgcact tccggagcca
     2941 gcgccttcgc cagtactttg gtcagcatca ctacacctgc tttcgaaacg gagtgcgcaa
     3001 tgtatccagg ccagccgagc agcccgccgg tatccgcgaa attgatgatc gtcccgttag
     3061 ttcgccggag aagctgcgcc gcggcttgcg cgcagaaaaa aggcgctttc agattcgaat
     3121 cgagtgaggc atcccagatt tcctccgtag tggaaatgat gctcgcggga aggaaattgg
     3181 ccgcgctatt gacgaggatg tcgagccggc cgaaccgctt tgccgcttcg tcaaagaggc
     3241 gcttgatgtc agcgacgctg cttaaatcgg cggcgatcgc gcggcctcgc ggccccagct
     3301 tttcgatttc ggcgaccgcc gactgcgcgt ccaatgttga agaacggtag tggataatga
     3361 cgtccgcgcc ctcttccgcg agacgcaacg ccaccgctcg ccccaggcgc tttgcagacc
     3421 cggtgacaag agcaacgcgc ccttgcaagg actcccctga cattttgccg tctcccttca
     3481 tcttcgaacc tgaaaccgta gcacattccc gtaagacagg cattcctgcc tgtcctcttt
     3541 cattttccga cgaagtttat acacgacaaa caaaattgtc tgtcctaccc tacaatcagc
     3601 acacgctgga ggcctgatga atctcgatct attggcaatc gccgcgcacc ccgacgacgt
     3661 ggaactcacc tgcggcggca cgctgctgaa gatggcgcag cgcggataca aaacgggaat
     3721 cctcgattta acctcgggcg aaatgggaac gcgcggcacg ccggagactc gcgcgaaaga
     3781 ggcagcgcgc gcggcgaaat tactcgccgt aaagtggcgc ggcacacttg gagtgcccga
     3841 ttccgacgtt cagccctccc gtcagcacaa gctgcggctc gccgcggtga ttcgtcagct
     3901 ccgtccaaag accgtcatcc taccctactg ggaagcgcgc catcccgacc attaccatgc
     3961 ctcgaccctc ggttacgagg ggtgcttcct ggcgggactg aaacaactgc ccatcgaagg
     4021 cgaacccttt cgccctttca agatcctgta ttccatttcc tacgccgacg tccggcccac
     4081 cttcgtcgtg gacatcactg cccaatacca gcagcgccgc aaggccatcc tggccttcgg
     4141 ctcgcaattc cggcccaaga agagcgaaaa gaaaagcaaa gtccacctgg ccatcgaccg
     4201 gctggaagcg gagatgaatc agttagcccg tcactatggc caaatgattg gcgtggacta
     4261 cggcgagccg ttcctgcaaa aggaactgat gaaggtcgag gatgtcgtga agatggaggt
     4321 tcggtcgatc tgaagctcaa aacttctagt catcctgtgc cggagaaaac ccgggaatgg
     4381 tttgccctgc cccggcgcgc tcgacccgga cacgggtttg cgcctcgtcc tcggcgccgg
     4441 acttgcgcag aatgaccatg gtatttagcg cctcggaata cagccgcgtc accaggtaaa
     4501 tgtccttcgt gccgtccttc ttccaaagct ggccaatctg gatcttcgag gtgctcatag
     4561 gggaccggct ctgactgtag agtgcgccgc gaagtgtgtc aactccctcc cgaggcagga
     4621 ggacgcaatc agtacgccgg tgacagaacc tccgtcccct ctgaccaacc tcctccactc
     4681 gggatacctc cttctgcctg aaaccctctg gaacctcctg ggaataggaa accttcctgg
     4741 accgtctaac atcctagtag ctatggaaac atcgtagggg cctgcccagc aattgcaggt
     4801 gtataatcct aggtaggcgg aggtacacga tatgcgagac gtcctgcggc ataccctgaa
     4861 gctggcgcta gttatcgccc tcgtagcggg ttcgaccttc gctgcccccc aaagtcaaga
     4921 tcccccgagc caggatcctg caaagacgcc accgccgcca gcccaacagc agacccagca
     4981 gcaaagcagc ccccagccgg tccagcagca agacacggtg catccgaaga attcaaagga
     5041 agacgtcgag gcgatcggca accgtagcgt cggaaaaggc atgaatctct actcgctgga
     5101 gcgagaaatc tccctcggga agtcactagc ccaggaagtc gagaagtctt cgaagttgat
     5161 tgacgatccg atcgtcaccg aatacgtcaa tcgcgtcggc cagaatctcg tccgcaattc
     5221 cgatgcgcgg gttcccttca ccatcaaagt gatcgattcc gacgaagtca acgccttcgc
     5281 gctgcccggc ggcttcttct acgtgaacag cggcttgatc atccgcgccc aggaagagtc
     5341 cgagcttgca ggagttatgg cgcacgagat ttcccacgtc accgcccgcc acggcacgaa
     5401 aaacgccacc aagggcgaat tgatgcagct cgccaccatc ccactgatct tgctcggtcc
     5461 cggcggctgg gccggctacg gcatttatca gggcctgaat ctggcgattc ccgtcaccta
     5521 cctgaagttc agccgcgacg ccgaacgcga ggcggattac ctcggcttgc aatacatgta
     5581 caaggccggc tacgacccca attcctacgt gactttcttc gagcgcatcc aggccgatga
     5641 aaagcgccgc ccgggcacca tccccaaggt tttctcatct cacccgccca cgccggagcg
     5701 catcgaaaac acccaaaagg aaatcgcccg catcctcccg gcgaggcagg aatacatcgt
     5761 caccacttcc gaattcgatt ccgtgaaggc tcgcctgcgc aacatcatgt tctcgcgcaa
     5821 agttacagac aacgcgcccg gcaagcccac cctgcgcacc aagaccgagc aatccaagaa
     5881 gcagccgggc agcactgatc ccaatagcac cgacgacgac cggccaacgc tcaagcgccg
     5941 ccccgattct cagccgtaat ctcgtaggct ttcgtgctct tttgctgtta tgtggaggcc
     6001 gggctttagc ccggctactt ctttttctta cgcgcggttg tagcggcgag ttcatcgcac
     6061 cactccgctc cctgctcatg tagcggcccc gggggcgcgt ggtcgactgg gttcccactg
     6121 agtttttctt caccgcccgc acgatgagag gacactcctt ctgcaatcca cggcaaattg
     6181 atccaatgag ggcgccccgc cctttccggt tttcttttcg aaagtgtggg ctctttctct
     6241 tatctctttt agctggtggt tcaccaacct attcctcttt ttacgacgcc gcccgatcgg
     6301 aagtcgccac tcctaaattt caccgcgcgt tgacaaaacg agggtgcccc accctttcgg
     6361 gtttgctttt cgaaagggtg ggttcttgct cctatcttct ctttttcgtt atttctcgcc
     6421 atcgttttct gtcttctcac cttcctgatc cgccttccta gtcgactgga tcaatcgtca
     6481 ccaatccagc atccccgtgc gcatagcacg gccagttgct ccacggccaa tccctcggat
     6541 gcgggactag ttttctctgc accggattcg cgttcatgta gttcaatttc tccctcttct
     6601 tctgcgaact ccat
//
LOCUS       DASYVO010000054         1944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_199232, whole genome shotgun sequence.
ACCESSION   DASYVO010000054 DASYVO010000000
VERSION     DASYVO010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199232"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(466..1551)
                     /locus_tag="VGR03_01915"
     CDS             complement(466..1551)
                     /locus_tag="VGR03_01915"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV1993062.1"
                     /translation="MIAIGVDVHKRQCTVAMQAEDGKVRFFDPMPNTREGWGELLEQL
                     PPETEIALEVSTSGYFAMSVLEEAGWLERAHWVHTAGIDSLRKQKYDRLDAERLARKL
                     SVAKQDPLPEAWFPPPQIRELRLRARQRCWLAVLRTQVKNRVQSLLQMHGLRPTVSDV
                     FGVAGRAWLAAQVPLLPAATRDSVEQLLRVLDFLLQELEKAEGYLRAVDGQFPELARL
                     RTIPGIGEILSPVIWSEIGRLERFQSARALVNYTGLVPSLYESGDVSIRGGITHQGSA
                     WLRWALVQAANAVMLGVNPFSRKYRRLRRRKISNVAKTAIARSLARCVYGVLKSGGNY
                     QAARWGRRPAGMVRSSGAGKPEVAIGH"
BASE COUNT          361 a          649 c          535 g          399 t
ORIGIN      
        1 cgatgtccgc accaggaaaa tcgtggccgc gttacaggat gaaaccggcc gcccggtaca
       61 gagcgagaaa ttgctcgaca tcacgatctc gaacggcaaa gtggtgcacg cgggcaatca
      121 attcggcgtt ggaatgaagg cgaagtaatg cagtaggtag ccatctctga aagcgcgatg
      181 ccgtgtcaag ctgattttaa gaaacacggc gccgcttgcg ggacgcgtga cacgcccgcc
      241 tgattttctg gtccatctga gttccgccgc ggcgtggcac gtggcctgct ggcggcgcct
      301 tccagttctg gttcttgctc ttgccgaccg cgccctctta gaggggcaca gggcgcgggc
      361 ggccttctcc cgtcgagttg ggtttgttcc cagtcatctc tcaagggtca aggccctcat
      421 gcggttgttc tgaatcggga gcaggtcctc atcacgacac ggtctttaat gacctatggc
      481 tacctcaggc ttgcctgctc ccgaacttct gaccatcccg gcgggacgac gtccccagcg
      541 tgccgcctgg tagttgccgc ccgacttcaa aactccgtac acacaacgcg ccagcgaacg
      601 ggcgatggcc gtcttggcca cgttggatat tttgcgtcgc cttaagcggc gatactttcg
      661 cgaaaacgga ttcactccca gcatcacggc gttcgccgcc tgcaccaagg cccagcgcag
      721 ccacgccgaa ccctgatgcg taatcccgcc gcgaatcgaa acgtcgccac tctcgtagag
      781 actggggacc agccccgtgt agttcaccag cgcccgggcg gactgaaacc gttccagcct
      841 gccgatctcg ctccaaatca ccggcgagag gatctcgcca atcccgggaa tcgtccgcag
      901 ccgcgccagt tccgggaact gcccgtccac cgcgcgcaga tacccttccg ccttctccag
      961 ctcctgaagc aggaaatcga ggacgcgcaa caactgctcc acactgtcgc gcgtggccgc
     1021 cggtaagagc gggacttgag cggccaacca cgctcgtcct gcgactccga aaacgtcgct
     1081 caccgtcgga cgcagcccgt gcatctgcag caagctctgc acccgattct tcacctgggt
     1141 tcgcaacacc gccagccagc accgctgccg ggctcgaaga cgaagctcgc gaatctgcgg
     1201 cggcggaaac cacgcctccg gcaacggatc ctgcttcgcc accgagagct tgcgcgccag
     1261 ccgctcggca tccagccggt cgtatttctg cttcctcaaa ctgtcgatgc ctgccgtatg
     1321 cacccagtgg gcccgctcca gccagcccgc ttcttccagc acgctcatcg caaagtaacc
     1381 gctggtcgaa acctccagcg cgatctccgt ctccggaggc agttgctcga gcaactcccc
     1441 ccatccctcc cgcgtgttcg gcatcggatc gaaaaacctt accttcccgt cttccgcttg
     1501 catcgctacc gtgcattgcc gcttgtgtac gtctactcct atcgctatca tggtttcgcc
     1561 tcctcgaaga attcactttc ttcctgcagg cccagtttac ctggacccga ggaggcatcg
     1621 cgctttcata acatcacggc acgctcttgt gccgtgggtg ggaagtggta gcgcatccat
     1681 aagtgaatcg gccgtgtcaa gcggcgtttt ttgctctttt tgagaaagga ccgccatgag
     1741 cgcggtggga atgtgaaaat cccgtggggg attttcaagc gcggtgggaa cggtgggaaa
     1801 gtccgttttg cttttaggac ttttccacgg cgcgcggcca cggtgatctt tctctcctgc
     1861 cgctccggac tccgactttc gcgaggcttg caccaggcat ccattcgtcc cgatcggatc
     1921 ggaacactct gtcggattca gact
//
LOCUS       DASYVO010000055         4067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_199238, whole genome shotgun sequence.
ACCESSION   DASYVO010000055 DASYVO010000000
VERSION     DASYVO010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199238"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VGR03_01920"
     CDS             complement(<1..314)
                     /locus_tag="VGR03_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993063.1"
                     /translation="MPSTRRAFLQSLSRSAIVLPLEKVLALARPREWLPAFPFQGTAS
                     PRTAAAGPPNDLGISFLNVARESGLNAKTIFGGEHKNKYLLETTGCGVAFYDYDNDGW
                     LD"
     gene            389..3091
                     /locus_tag="VGR03_01925"
     CDS             389..3091
                     /locus_tag="VGR03_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993064.1"
                     /translation="MQAHDAAGATRMHVTPGTPVHSQRQFQWTLLRLFGACIAATLIM
                     GIGAPAGLGQQAREKPRDLELSRAVRSWEFLSVTGTRAGLLGNESGRMEAWVYPLKIF
                     RDFHLKFHTEGRVLAAEALARTVTVRPESAIILYAGDTFTVRETFFVPVREQGAIVIL
                     DVETERPLEIEAAFHRDFQLEWPAALGATYLDWVAERRVFYFGEEQRKFAALAGSPTA
                     TEPRSEFQTNYSEAQESSFRLGPTTKGKESKLIVIAGSLDGRAAAEKTYQHLTTDYAQ
                     LLKESAEYYRAYLEKTVSVELPDAQIQRAYDWARVSMLQGVVNNPSLGTGLVAGYRTS
                     GESQRPGFAWFFGRDSFWTSFALNASGDFSNSRAALEFISKYQREDGKIPHEISQGAS
                     FVNWFKDYPYPYASADATPLYIIAMNDYVVESSDTAFAKEKWESLRKAYEFLKSTYDG
                     RGLPQNFGVGHGWVEGGPLLPVKTEYYQSGLGAEALRALSNLARVTGQEEMSKALEKD
                     FEKQRSLVNESFWIADKKRFAFALDKNDKKVDEPSVLATVPMWFGLPSEADAAPMIQQ
                     LADFDHQTDWGMRIISNRSPLFSGGGYHYGSVWPLFTGWASVAEYRYHQPFPAYANLR
                     ANALLALDGSLGHVTEVLSGDYYQPLSTSSPHQIWSAAMVVSPILRGMLGLETDAIQR
                     RVRLAPHIPADWTRFTVQNVQLGKNTLLMNYSKTDEGIVLETGLTSGSEECTVEFRPA
                     ISLRAKVRTVELNGKPVPFQVETRGGDQHIVVQFPVTAGKYFLRIRLLNEFGLSEQSN
                     LPALGSASRGLRILSETWSASKDQLTLEVSGAAGGEYGLRVWNPGEIQSVEGAGLNWG
                     IKNPSAVRETLWIQIPASDLEPYPHVKVVIHFSAGQKKGKPEKH"
     gene            3248..>4067
                     /locus_tag="VGR03_01930"
     CDS             3248..>4067
                     /locus_tag="VGR03_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEV1993065.1"
                     /translation="MFRKILVVGVLIMGLSACAPWAGAQQQTVDAEGHPWWQHAVFYE
                     IYPRSFADSNNDGIGDLNGIASKLDYLKELGVDAVWITPCFPSPQVDFGYDVSDYEAI
                     DPMYGTLKDFDHLVGEGKKRNIRIILDFVVNHTSDQHKWFLDSKSSKTSEHRDWFIWR
                     DGKGPGQPPNNWLSTFGLSAWKLDPKTNQYYYHYFYPEQPDLNWRNPAVEKAMLDAMR
                     FWYKRGVSGFRLDAVDTLFEDPKLTDNPVLAGTDKFGRPNMDEKYNKKLPEVHDV"
BASE COUNT          900 a          966 c         1340 g          861 t
ORIGIN      
        1 atatccagcc agccgtcatt gtcgtaatcg tagaaagcga cgccacaacc ggtggtttcg
       61 aggagatact tgtttttgtg ctcgccgccg aagatggtct tggcgttaag gccggattca
      121 cgcgcaacgt taaggaagga aatgccgaga tcgttggggg gaccagcggc ggcggttcgt
      181 ggagaagccg tgccctgaaa cgggaatgcg ggcagccact ctctcggacg tgccagggca
      241 agcactttct ccagcgggag cacgatcgca gagcggctga gtgattggag aaatgcgcgg
      301 cgtgtggaag gcaaaagaag cgataccttt ccttgcgagc cccgtgctag aatccggcgc
      361 tttcctttat agtaaggcca acgaggcaat gcaagcacac gacgcagcgg gggccaccag
      421 aatgcacgtc acgccgggaa cacctgttca ctctcaaaga caattccaat ggacgctgct
      481 gagactgttt ggtgcatgca tcgcggccac gctgatcatg ggcatcgggg ctcccgccgg
      541 tttgggccag caggcgcggg agaagccacg cgatcttgag ctttcgcgcg cagtgcgctc
      601 gtgggaattt ctgtccgtca cgggaacacg cgcgggactg ctggggaatg aatccggccg
      661 catggaagcg tgggtgtacc cgctgaagat ctttcgagac tttcatctga agtttcatac
      721 cgaagggcga gtcctagcgg ctgaggcact ggcgcggacg gttacagtgc ggccggagtc
      781 ggcgattatt ctttacgcgg gggacacatt cacggtgcga gagacatttt tcgttcccgt
      841 gcgggaacaa ggcgccatag tcattctcga cgtggaaacg gaacggcctt tggagatcga
      901 ggcggctttt catcgcgact ttcaattgga atggccggcg gcgctcggag ctacttatct
      961 ggattgggtt gccgaacggc gcgtgtttta ttttggcgag gaacaaagaa aattcgcagc
     1021 gctggcgggt tcgccgacag caacggaacc gcgctccgaa tttcagacaa attattccga
     1081 ggcacaggaa agctcgttcc ggctgggccc cacgacaaaa ggaaaagaat ccaagttgat
     1141 cgtcattgca ggctcgctgg atgggcgcgc ggcagcggag aaaacgtatc agcatctgac
     1201 gaccgactac gctcaattgt tgaaggaatc ggcggaatac tatcgagctt atctggagaa
     1261 aacggtgagc gtggaactgc ctgatgcgca gatccagcgg gcctacgatt gggcgcgcgt
     1321 aagcatgttg caaggtgtgg tgaataatcc ttcgctgggg acgggcctcg ttgcaggata
     1381 ccgaacgtcc ggagaaagcc agcggccggg ctttgcctgg tttttcgggc gcgactcgtt
     1441 ctggacttcc ttcgcactca atgcctcggg tgatttttcc aattcacgcg cggcgctgga
     1501 gttcatcagc aaataccagc gcgaagacgg caagatcccg catgagattt cgcaaggcgc
     1561 aagttttgtg aattggttca aggactatcc gtatccctac gcttcggcag atgcgactcc
     1621 tttgtacatc atcgcgatga atgattacgt cgtggagagc agcgacacgg cgtttgcaaa
     1681 ggaaaagtgg gagagtctgc ggaaggctta tgagtttctg aaatccacgt atgatgggcg
     1741 gggactgccg cagaattttg gcgtagggca cggatgggtg gaaggcgggc cgctgctgcc
     1801 ggtgaagacg gagtattacc aaagtgggtt gggggcggaa gcgctacggg cgctttcgaa
     1861 tctggcgcgg gtgacgggcc aggaggagat gagcaaggcg ctggagaaag attttgagaa
     1921 gcagcggtcg ttggtgaacg aaagcttctg gatcgcggac aagaaacgat ttgcgtttgc
     1981 cttggacaag aacgacaaga aagtggacga gccgagtgtg ctggccacgg tgccgatgtg
     2041 gttcggcctg cccagcgagg cggatgcggc gcctatgatt cagcaactgg cggattttga
     2101 tcatcagacg gattggggga tgcggatcat ttcgaatcgc tcgccgctct tcagcggcgg
     2161 cgggtatcac tacggttcgg tgtggccgct gttcacggga tgggcgtcgg tcgcggagta
     2221 tcggtatcac cagccgtttc cggcgtacgc gaatctgcgg gccaacgcgc tgctggcgct
     2281 ggatggctcg ctagggcacg tgacggaagt tctttccggt gactattacc agccgctctc
     2341 gacgagctcg ccgcaccaga tttggtcggc ggcgatggta gtgagcccca ttttgcgcgg
     2401 gatgctgggg ctagagaccg atgcgatcca gcgcagggtg agattggcgc cgcatattcc
     2461 ggcggattgg acgcgattca ccgtgcagaa tgtgcagctc ggaaagaaca cactgctgat
     2521 gaattattca aaaacagatg agggaatcgt gttggagacc ggcctaactt cgggaagcga
     2581 agagtgcaca gtcgaatttc gtcccgcgat cagtttgcgg gccaaagtgc ggacggttga
     2641 gctgaatggg aagccggttc ctttccaagt ggaaacgcgc ggaggcgatc aacacatcgt
     2701 ggtgcagttt ccggtcacgg cgggaaagta cttcctgcgg attcgtctgc tgaatgaatt
     2761 tggactgagc gagcaatcta atttgccggc gcttggaagc gcgagccggg ggctgcggat
     2821 tctttcggag acgtggtcgg cttcgaagga ccaactgacg ttggaggttt ctggagcggc
     2881 aggcggcgaa tacggactaa gggtctggaa tccaggcgag attcagagtg tggagggggc
     2941 aggtttgaat tggggcatta agaatccgag cgctgtaaga gagacgctct ggatacaaat
     3001 tccggcgagc gatttggagc cgtacccgca cgtgaaagtt gttatccatt tttcggcggg
     3061 gcagaaaaaa ggcaagccgg aaaagcacta attcaccggt gaatgtggtt ggtggcttcc
     3121 tacgttgagg tcctgagagc atcctcaggg gatgccctca ggataacacc gcgcttagtt
     3181 ttggagtgta gcgacgaaat ggttgggcag tgcagtggtg agagagatga gcaagggagg
     3241 aagaggcgtg tttagaaaga ttcttgtagt gggcgtattg attatgggcc tgagcgcatg
     3301 tgctccttgg gcgggagcgc agcagcagac ggtggatgcg gagggccacc cgtggtggca
     3361 gcacgcggtg ttctacgaga tttatccgcg aagttttgcg gacagcaata atgatgggat
     3421 cggcgacctc aacggcatcg cttcaaaatt ggattatttg aaagagctcg gcgtggacgc
     3481 cgtgtggatc acaccgtgtt ttccgtcgcc tcaggtggat ttcggatacg acgtgtccga
     3541 ctatgaggcg attgatccca tgtatggaac gctgaaggac ttcgatcacc tggtgggcga
     3601 aggaaagaag cgcaacattc gaatcattct ggattttgtg gtgaatcaca cgtcggatca
     3661 gcacaagtgg ttcctggatt ccaagtcttc caagacctcc gagcatcgcg attggtttat
     3721 ctggcgggac ggcaaggggc cgggccagcc gccgaacaac tggctttcca cgtttgggct
     3781 gtctgcatgg aagctcgatc caaagacgaa tcagtattat taccactact tttatccgga
     3841 gcagccggac ttgaactggc gcaatcccgc ggtggaaaag gcgatgctgg acgcgatgcg
     3901 cttctggtac aagcgtggcg tttccggatt ccgcctggat gcggtggata cgctctttga
     3961 agatccaaaa ctcacggaca atccggttct tgcgggcacg gacaaattcg gccggccgaa
     4021 catggatgag aagtacaaca agaagttgcc tgaagttcat gacgtga
//
LOCUS       DASYVO010000056         1579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_157134, whole genome shotgun sequence.
ACCESSION   DASYVO010000056 DASYVO010000000
VERSION     DASYVO010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157134"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VGR03_01935"
     CDS             complement(<1..281)
                     /locus_tag="VGR03_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993066.1"
                     /translation="MNASLADANSSPITPLRGTPSRMNGRLLVWAITSALAGFLFGFD
                     TVVISGAEQTIQGLWGLSAGVHGIAMAAALYGTVLGSLTGGLPADRFGR"
     gene            complement(818..>1579)
                     /locus_tag="VGR03_01940"
     CDS             complement(818..>1579)
                     /locus_tag="VGR03_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993067.1"
                     /translation="NIWIKRLDRGPSIKLTQEGNWNVGPAWMPDGKSVTFSSDVAAGA
                     FHLWTKRADGSAPAVLQFREKRNVYGPRWSPDAKWLIFQTDPAQSGAGDILGIRPGID
                     SAPVALVATRFTEVSPALSPDGRWLAYASNETGEYKIYVVPFPNTGAAKWAISTGGGT
                     EPLWSHRGNELFYRDASKNLVAAELKTKPTFSVGRSTALFPAAEFHSFQFALQYAVGS
                     DDRRFLMIRPVETSTTDKLIVVENWFEELKAKPQK"
BASE COUNT          331 a          501 c          452 g          295 t
ORIGIN      
        1 ttgcggccga accgatccgc gggcagtccg ccagtcagcg aaccgagcac ggtgccatag
       61 agcgccgcgg ccatcgcgat cccgtgcacg ccggcactca ggccccagag tccctgaatc
      121 gtctgctcgg caccggaaat gacgacggta tcgaaaccaa agagaaaccc ggcgagcgcg
      181 gaggtgatcg cccatacgag caaccggcca ttcatccgtg acggggttcc gcgcaacggc
      241 gttatcgggc tgctgttcgc atcagcgaga gaagcgttca tagtggtctg caggggtctg
      301 cgcagcgggc cgtgtcaaga tgcgccatcg tgatccgcaa ccgcaacgca ccgagagcgg
      361 ttttcagtcg tcgcccgcca tgcgtccacc accaacactc tttctggtct gtgggctacc
      421 aggcgccggc aagacacgaa agcacttccg acaccgcgct ccggcgctgg gcgctcgaag
      481 ttcgttacgt tatgtcgcgg ggaacggaca ccacgaatgg acaccacgga ccaatccggc
      541 acaaaagaga aaaaccccgc cagcgcctaa acgcttgcgg ggtaatgatc tgaggtttat
      601 gggcctgggt agagttgaac taccgacctc acgcttatca ggcagcttca tacgaacatg
      661 aattcggctc agaagttctt actagcctgc ctttcacgaa catcctgctc agcatcccgt
      721 gcatttgaga acatcttgcg cacttccgcg aacattttgc gcacgcgaat ggacaccaca
      781 accgacacca tcgcgatccg ggagcaagtg agctagttca cttctgcggc ttcgccttga
      841 gctcctcgaa ccaattctca accacgatca gcttgtcggt cgtgctggtt tccaccggcc
      901 gaatcatcag gaagcgccgg tcgtctgacc ccacggcgta ttgtagggcg aattggaagg
      961 aatggaattc cgcggcggga aagagtgccg tggagcggcc gaccgagaat gtcggtttgg
     1021 tctttagctc ggccgccacg aggttcttgg atgcgtcgcg gtagaacaac tcgtttccac
     1081 gatgggacca aaggggctcc gtccctccgc cggtcgaaat cgcccacttt gccgcacccg
     1141 tgtttggaaa agggaccaca tagatcttgt actcaccggt ctcattcgag gcatacgcca
     1201 gccaacgtcc gtcgggtgaa agggccggag acacttccgt gaacctggtc gccacaagcg
     1261 ccaccggcgc tgaatcaatg cccggccgga tcccaagaat gtcacccgcg ccggactgcg
     1321 ctgggtcggt ctggaagatc agccattttg cgtcaggaga ccatcgcggg ccgtacacgt
     1381 tccgcttctc acggaactgg agcactgccg gcgcgctacc atcggcccgc ttggtccaca
     1441 gatggaaggc gcctgcagcg acatccgagg aaaacgtcac cgatttcccg tccggcatcc
     1501 acgcagggcc aacattccag tttccctcct gcgtcagctt gatgctcggg cctcggtcca
     1561 gtcgcttgat ccagatgtt
//
LOCUS       DASYVO010000057         4088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_141750, whole genome shotgun sequence.
ACCESSION   DASYVO010000057 DASYVO010000000
VERSION     DASYVO010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141750"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="VGR03_01945"
     CDS             complement(<1..1418)
                     /locus_tag="VGR03_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993068.1"
                     /translation="MPEQKIYLDDNGNPMPPPKVYLDENGNPLGAAAGPQPSALEALK
                     AQVATYPKDIGSSTGFTSAFTGSVPPEKQTGPMAAVGRLARSPLDMLKMAAAPADPSK
                     GELETPGAPIGLQRVIGSQVGLLASGLKGLTRTPDPDAIGTQTRMNAGQPSPRDLENL
                     HAAGETLAGATPLAGPWAANMGERAGRGDLSGTATEFAGGLAAPKALHGVIDLAKDAS
                     GAYSVPKPKALAPAASPAESVNPLQAYHAASEHGIDMLPAQVSDSRVAKIADSIGKRA
                     VFDLGDLKGKGERQNIALSKWHQDAGNQVDPGGTAQNTESIGRDLQAGAEARYAAEKA
                     AVAARGPNVPGVASTIMDGMAPRASAEEVAGKVQNRLQATVAQLGHQADSIFGDLDTK
                     ARGVTVAASNGQKLAQKIIQENGSYYEKFPSVAPTQALKLLQRIASTDKPLTFTEAQK
                     LRSDALELARNNPDIFKGKPAG"
     gene            complement(1670..3220)
                     /locus_tag="VGR03_01950"
     CDS             complement(1670..3220)
                     /locus_tag="VGR03_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993069.1"
                     /translation="MADDLITNPGSDDELRKRRIFQALLGTQGAAVPAAPSTDAVGST
                     VSGPLSASNVPAPLPSGSGGPAPIPGPIKPPAAKLPGDGLPSPAAVAPTSAAPIGRPT
                     PAPGSSPYDAQIASDRSKLDDLSKPRPLWQQILRGAASTLPIVGPTMAYDRNIKEKRR
                     DQLISDIHNQQEMGLRKQDFDIQQGNLQANRENTAEWHRQSVEQRRERNPEEEFASRQ
                     RIAEIIKMNLSDPAVQGWLASGSGSPIPRGKAPTTAIGKFIFDHPDATDDDLAAFAQK
                     LGKGRGAGSLDEQYLTALESGDSAKAALIKQTLETTHPRSMTINPPSATGTWTLQEGE
                     GGKSVLLNSKTGETKAAPEGVVPKGTALHVKADYDKTMAPVEGAIGYASSYLANKVFT
                     GSGDEALQEKFFDLAKPSSGFRMTQPQMQMLQESRDWMGGMAARVRHATTGTWFSEEQ
                     RSQIVQTMKDLAAAKSKSFGAGGRGQAAAPAAAGPAAPATLRKVGDTKQFANGKTGKW
                     DGVGWVNQ"
     gene            complement(3264..4067)
                     /locus_tag="VGR03_01955"
     CDS             complement(3264..4067)
                     /locus_tag="VGR03_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993070.1"
                     /translation="MGGFSGINVDVGSGASRGMPWQNPGRRKPQQPITGPIRPETAPA
                     GTTMPGSFASPADGGATPGPLTPQNVPGYSNVNTGTKNAFADLAGQDQANQNKALNST
                     TPVLKEAMSNANPAAVEASPFYRALKNQAIESTSASYDNERANSRARANASGFGYASP
                     VAAGAENEIGAREAGALARAPEQALLETPGFIAQTEAPGLAAARTTTAQGGQYGQEAS
                     QLFGDQLSWQEQQKAIDAQRMMHKTDLQFELYKQWLQGAQSGAAGAAGG"
BASE COUNT          673 a         1356 c         1108 g          951 t
ORIGIN      
        1 cagcccgccg gctttccttt gaaaatgtcc ggattgtttc tcgccagctc aagcgcgtcc
       61 gatcggagct tttgggcttc ggtaaacgtc agcggtttgt cggtgctcgc gatccgctgt
      121 aacaatttca gcgcctgcgt cggcgccacg ctcgggaact tttcgtaata ggagccgttc
      181 tcctggatga ttttttgggc gagcttctgc ccgttcgaag cggcaaccgt cacgcccctc
      241 gcctttgtgt cgaggtcgcc aaagatcgag tcggcctgat ggccgagttg tgcgaccgtg
      301 gcctgcagcc tgttctgtac cttgcccgcc acttcctccg cgcttgcacg cggagccatg
      361 ccatccatga tcgtcgaagc cactccgggc acgttcggcc cgcgcgcggc gaccgccgct
      421 ttctccgccg cgtaacgcgc ctccgccccg gcttgcaaat ctctcccgat gctttcggtg
      481 ttttgcgcgg tccctccggg gtcaacctgg ttgcccgcat cctgatgcca tttggaaagc
      541 gcaatatttt gtctctcccc tttacccttc aaatccccta aatcaaaaac ggcccgcttc
      601 ccgatcgagt ccgcaatctt cgcaaccctg gaatcactca cttgtgcggg cagcatgtcg
      661 atgccgtgct cgcttgccgc gtgatacgcc tgcaacgggt tcactgattc cgccggcgat
      721 gcagccggcg ccagcgcttt cggtttcggc acgctgtatg ctcccgatgc gtccttcgcc
      781 agatcgatca cgccatgcag ggcttttggc gcagcaagcc cgcccgcaaa ttcggtcgcc
      841 gttcctgata ggtctccgcg tcccgctcgc tcccccatgt ttgccgccca gggtcccgcg
      901 agaggagtag ccccggcgag ggtttctccg gctgcatgta aattttccag gtcgcgcggc
      961 gaaggctgtc ccgcgttcat gcgtgtctgt gttccgatcg catccggatc gggcgtccgg
     1021 gtcagtccct ttaggccgct tgccagtaag cccacttgcg agcctatgac tctttgcagc
     1081 ccgatcggcg ctccgggcgt ttcaagttca ccctttgacg gatccgctgg agccgccgcc
     1141 atcttcagca tgtccagcgg cgagcgggcc agtctaccga ccgcagccat aggtccagtc
     1201 tgtttttccg gaggcacgct cccagtgaac gccgaagtaa aaccagtcga ggatccaatg
     1261 tctttcggat acgtggccac ctgcgctttc aaagcctcga gcgccgacgg ctgcgggccc
     1321 gctgccgcgc ccagcgggtt tccgttctcg tccaggtaca ccttgggcgg aggcatcgga
     1381 ttcccgttgt catcgagata tattttttgt tcgggcatct tttccctgac ggcggtttaa
     1441 taattcggag gcggctgctc ttctaattcg agatcgatta cgtgccagat gttgtcgcac
     1501 ccggggaaga aattgctcaa ataatccgcc aaattaaccg ccgcctgaag gctcccatga
     1561 acctctatga tctttgggaa ttgtgtccca aggcactcat aacgatcgtc gtctcgcaca
     1621 atatgccaag ttgtgtccct ctcgctgctg tccaatttac ccccctttgt tactgattta
     1681 cccaaccaac tccatcccac tttcccgtct tgccgttcgc gaactgtttc gtgtcgccca
     1741 ccttgcgaag cgtcgccggc gccgcaggcc ccgccgctgc aggagccgcc gcctggccac
     1801 ggccacccgc accaaacgat ttacttttcg ccgccgccaa atccttcatg gtttggacaa
     1861 tctgactgcg ctgctcctcg ctgaaccaag tgccggtggt cgcgtgcctg acgcgcgccg
     1921 ccatcccgcc catccaatct ctcgactcct gcaacatttg catctgcggc tgggtcatgc
     1981 ggaaaccgct cgacggtttt gccagatcga aaaatttctc ctgcagcgct tcgtcgccgg
     2041 accctgtgaa aactttgttg gccaaatatg aagaggcgta gccgatcgct ccttcaaccg
     2101 gagccatcgt cttgtcgtaa tcagccttta cgtgcagcgc cgtccctttc ggcactacgc
     2161 cttcaggcgc cgccttcgtc tctcctgttt tcgagttcag taagaccgac tttccacctt
     2221 caccttcttg caatgtccac gtgccagtgg cgctcggagg attgatagtc atcgatcgcg
     2281 gatgagtcgt ttcaagggtt tgtttaataa gcgcagcttt cgcagaatcg ccggactcaa
     2341 gggcggtgag atattgctca tcgaggctgc cggcaccgcg tcccttgcca agtttctgcg
     2401 cgaacgccgc gagatcatcg tcggttgcgt ctggatggtc gaagatgaac ttccctatcg
     2461 ccgtggttgg cgctttccct cttggtatag gagagcctga accgctcgcg agccaccctt
     2521 gcactgcggg gtccgataga ttcattttta tgatttccgc tatcctctgc cggcttgcaa
     2581 attcctcttc cgggtttctc tctcggcgct gctcgacgct ctgtcggtgc cattccgccg
     2641 tgttctcccg gttcgcctgc aggtttcctt gctgaatatc aaaatcctgt ttccgcagcc
     2701 ccatctcctg ctggttgtga atgtcgctga tcagttggtc gcggcgcttc tctttgatgt
     2761 ttcggtcgta agccatcgtg ggccccacga tcggaagagt ggaagccgcc ccacgcaaaa
     2821 tttgctgcca caacggccgt ggcttgctca agtcatcgag cttcgatcgg tcggaggcaa
     2881 tctgcgcgtc gtaaggcgac gaacctggcg ccggcgtcgg acgtccgatc ggagctgcag
     2941 acgttggagc aacggcagcg ggcgaaggca gaccgtctcc ggggagtttc gcagctggag
     3001 gcttgatcgg cccaggaatc ggagctggcc cgcccgatcc actcggaagt ggggccggaa
     3061 cgttcgaagc cgaaagcggc ccactaacgg tcgagccaac cgcatccgtc gacggcgccg
     3121 ctggtaccgc cgcgccctgt gttccgagca gcgcttgaaa gattcgccgc ttccgcagct
     3181 cgtcgtcact ccccggatta gtgatcagat cgtccgccac actgcctcct gaatcctaaa
     3241 tcctgaatcc cgaatccttc tccctacccc cccgctgccc ccgccgcacc actctgcgcg
     3301 ccttgcagcc actgcttgta aagctcgaat tgcaagtccg tcttatgcat cattcgctga
     3361 gcgtcgatgg ccttctgttg ttcctgccac gaaagctggt ccccaaagag ttgcgaagct
     3421 tcctgcccat actgcccgcc ttgcgcggtt gtagtcctcg ccgccgcgag tcccggagct
     3481 tcggtttgcg cgataaagcc cggagtctcg agcagcgctt gttccggcgc ccgagcgaga
     3541 gctcccgcct ctcgagcgcc gatttcattt tccgcgccgg cggccaccgg actcgcatac
     3601 ccaaagccgg aagcgttggc gcgagctcgc gagttggcgc gctcgttgtc gtaactcgcc
     3661 gaagtactct cgatcgcctg attcttaagc gcgcgataaa acggcgaagc ctcgacggcc
     3721 gcaggattcg cattactcat ggcctctttc agcaccggag tagtcgagtt caaagccttg
     3781 ttctggttcg cctggtcctg cccggccaga tcagcgaagg cattcttcgt cccggtgttg
     3841 acgttggaat agcccggaac gttctgagga gtcagcggcc ccggagtcgc accgccgtcc
     3901 gcgggcgacg cgaaagatcc tggcatcgtc gtgccagccg gcgccgtctc cgggcgaatg
     3961 ggtccggtga tcggctgttg aggcttgcgc cgccccggat tctgccaagg catcccgcgc
     4021 gaagcacccg atcccacatc cacattgatc ccgctaaatc cacccatgac gttttctccc
     4081 tcctaaat
//
LOCUS       DASYVO010000058         3396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_44318, whole genome shotgun sequence.
ACCESSION   DASYVO010000058 DASYVO010000000
VERSION     DASYVO010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44318"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="VGR03_01960"
     CDS             <1..1087
                     /locus_tag="VGR03_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993071.1"
                     /translation="FGSGKVLIFGHVIDKDGTPYAADIRGILKGFADQLHKKEGYTLN
                     AANEIEGFLFKGPDAERGYHETGRFEYVNTGGYYHSLPGDPLRTFIDTTAEVQRAMGF
                     QNEKDHPEVAPSQFEINYSYGEVVAAADQIQLYKLVCRQVATNMGLTACFLPKPVVGV
                     NGSGMHTNVSINKGGKNMFWDAKGEEKLSKMGWAFLDRILTHGNDICLLLNASVNAYR
                     RLDPHFEAPNQLNASPVNRGAMIRIPIGNEKSMRVEVRSVAPDANPYMVMYSIFRTGL
                     EGETASIKNLRQAGRYLPDNIYTALENFRSAEWTTKLFGADVKARYADLKQASADRCP
                     RSLGTIVKAPEVQFHHEVYNQFLWNKF"
     gene            1116..2045
                     /locus_tag="VGR03_01965"
     CDS             1116..2045
                     /locus_tag="VGR03_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993072.1"
                     /translation="MGRRASLPTLKADCKFLLEGGVFTEQLIDLMTNARSQILRQQQA
                     WAAKRKIAVDSSGYTGARDANLFQPLHPDTLADFQCGSGDELGKNGRRGKIQALHSSS
                     ALAVNVFDYWRGRSLAWVAGALPLTSEPSLLRFEAQFPTGLPGSPPNLDLAFVLADRQ
                     TVAVESKFAEPYARANQAALFKRKYFPTGNGLWHDRALPRCQRLAVRLHRGELQFQYL
                     NAAQLLKHILGLAQPSVGQFTLFYLWYAVPSDEERQHGAEIKAFADEIGSELDFRALT
                     YQELFSSALRNLGAEHVAYLSYIGERYFPALRA"
     gene            2441..>3396
                     /locus_tag="VGR03_01970"
     CDS             2441..>3396
                     /locus_tag="VGR03_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEV1993073.1"
                     /translation="MKQSYNGQAIPANGKVIEYIGGELRVPDAPIIPFIEGDGTGRDI
                     WKASRRVFDAAVEQAYGGKRRIAWFEVFAGEKAFNAFQQWLPDDTVDAIRDFRVAIKG
                     PLTTPVGGGIRSLNVALRQILDLYACVRPVRYFPGVPSPVRHPEKMDVMIYRENTEDV
                     YIGIEWKSGSPEAKKLLEFLNNVMLKDTKKQIRWDSGVGIKPISPTGTKRLVRRAIRY
                     ALESGRKSVTLVHKGNIQKFTEGAFRDWGYELAREEFRAQTIAERESWIVDNLDKNPS
                     LTVEQNAAMIEPGLDRATEDLRKSVCEEVKRTLAAIYASHGHG"
BASE COUNT          715 a         1060 c          992 g          629 t
ORIGIN      
        1 cttcggctcc ggaaaagtgc tcatcttcgg ccatgtgatc gacaaggacg gcacgcccta
       61 cgccgccgac attcgaggca tcctgaaggg cttcgccgac cagcttcaca agaaggaagg
      121 ctacacgctg aacgccgcga acgagatcga aggattcctc ttcaaagggc cggacgccga
      181 gcgcgggtac catgaaaccg gccgcttcga atacgtcaac acgggcggct attaccactc
      241 cctgccgggc gacccgctgc gcactttcat tgacaccacc gccgaagtcc agcgcgccat
      301 gggcttccag aacgagaagg accaccccga agtcgctcct tcgcagttcg agatcaacta
      361 cagctacggc gaggttgtcg ccgccgccga ccagattcag ctctacaagc tggtgtgccg
      421 ccaggtagcc acgaacatgg ggctgacggc ctgtttcctg cccaagccgg tcgtcggcgt
      481 gaacggcagc ggcatgcaca ccaacgtctc catcaacaaa ggcggcaaga acatgttctg
      541 ggacgccaaa ggcgaggaaa aactcagcaa gatgggctgg gcgtttctcg accgcatcct
      601 gacgcacggc aacgacatct gcctgctgct gaatgccagc gtgaacgcct atcgccggct
      661 cgacccgcac ttcgaagctc ccaaccagct caatgcctcg ccggtgaacc ggggcgcgat
      721 gatccgcatc cccatcggca acgaaaaaag catgcgcgtg gaggtgcgct cggtggcccc
      781 ggacgcgaac ccttacatgg ttatgtattc gatcttccga acgggcctcg agggcgagac
      841 cgccagcatc aagaatcttc gccaggccgg acgctacctc ccggacaaca tttacacggc
      901 cctggaaaac ttccgcagcg ccgagtggac gacgaagctg ttcggcgcag acgtgaaagc
      961 ccgctatgcg gacctgaaac aggcttccgc ggaccgctgc cctcgatcgc tcggcacaat
     1021 cgtcaaagct cccgaggtgc agttccacca cgaggtgtac aaccagttcc tctggaacaa
     1081 gttttaggag tggtcgcacc cttcagtgac ccggcgtggg tcgccgcgca tccttgccga
     1141 cgcttaaggc cgactgcaaa ttccttctcg aaggcggcgt cttcaccgag cagctcatcg
     1201 atcttatgac aaacgctcgc tcacagatac ttagacagca acaagcctgg gcggccaagc
     1261 gcaagatcgc agtcgatagc tccggttaca ccggtgctcg cgacgccaat ctgtttcagc
     1321 cgctgcaccc cgacacgctg gcggactttc aatgtggcag cggagacgaa ctgggcaaga
     1381 atgggcgccg ggggaaaatc caggcactgc attcgtcatc ggcgctcgct gtaaatgtct
     1441 tcgactactg gcggggccgg tcgctggctt gggttgcggg cgcgctgccg ttaacttcgg
     1501 agccttcatt gctgcgcttt gaggcgcaat tcccaacagg gctaccagga agcccgccaa
     1561 atctcgattt ggcgtttgtg ctggcagacc gacaaacggt agccgtggag tccaagttcg
     1621 ccgaaccata cgcccgcgca aaccaagcgg cgctgttcaa acgcaaatat ttcccgactg
     1681 ggaatgggct ttggcacgac cgcgctttgc cgcggtgtca aagactagcc gtccgattgc
     1741 atcggggcga gctacagttt caatacctga atgcagcaca gcttcttaag catattctcg
     1801 gcctcgccca accctcagtg ggtcagttca cccttttcta tctttggtat gccgtacctt
     1861 ctgacgaaga gaggcagcat ggagcggaaa tcaaagcgtt tgccgatgag atcggttccg
     1921 aactagattt tcgtgcgctc acctatcagg agctgttttc ctcagcacta cggaatctcg
     1981 gggccgagca tgtggcgtat ctgagctaca ttggggagcg ctacttcccg gcattacggg
     2041 cttaatggat gcaggcagtg tggccggccc attctctttg caatacttcc gcaataatcc
     2101 ggcgtgcttt aatcggtttg tccggtttcg ccttgcgact tacctgtaac tgacgcgctg
     2161 tcaatccgcc cggccattcc acctgtacta cgacatctag gggcctgccc ggcggttggt
     2221 gttccgctgg gacattcgcg ggaatcgtat ggaaagcacc tcggttgagc cggggcgttt
     2281 tctatacccg ggcgcgatgg aagcgcgatc ggcacagctc ggtttttggc tgaggctgcc
     2341 gatgtgataa tgttctttgt tcgagcgagg cccgcggacc cctctttgtg cggaccagcg
     2401 gcactgcgat tcgcctccag atcggcaaga gggctcatcc atgaaacagt cctacaacgg
     2461 ccaggcgatt cccgccaatg gcaaagtaat tgaatacatc ggcggcgagc tgcgcgtgcc
     2521 cgatgcgccg atcatcccgt tcatcgaagg cgacggaacg ggccgcgaca tctggaaggc
     2581 ttcgcggcgc gtcttcgacg ccgccgtcga gcaagcctac ggcggcaagc gccgcattgc
     2641 gtggttcgag gtcttcgccg gagaaaaagc gttcaacgcc ttccagcagt ggttgccgga
     2701 cgacacggtc gacgccatcc gcgattttcg cgtggccatc aagggcccgc tcacgacgcc
     2761 cgtcggcgga ggcatccgct cgctgaacgt cgccctgcgg cagatcctcg atctctacgc
     2821 ctgcgtgcgc ccggttcgct acttccccgg agttccctcg ccggtgcgcc acccggaaaa
     2881 aatggacgtg atgatctacc gcgagaacac cgaggacgtc tacatcggca tcgagtggaa
     2941 gtccggctcg ccggaagcga aaaaactgct ggaattcctg aacaacgtga tgctgaagga
     3001 caccaagaaa cagatccggt gggattccgg cgtgggcatc aagccgattt cgcccaccgg
     3061 aacgaagcgc ctggtgcgcc gcgccatccg ctacgcgctc gaaagcggcc gcaagagcgt
     3121 cacgctggtg cacaagggca acattcagaa atttacggaa ggcgctttcc gcgactgggg
     3181 ctacgagctg gcgcgggagg agttccgcgc gcagacgatc gccgagcggg aaagctggat
     3241 cgtggacaac ctcgacaaga acccgagtct taccgtcgag cagaacgccg cgatgatcga
     3301 gccgggcctc gaccgcgcca ccgaagatct ccgcaaatca gtgtgcgaag aagttaagcg
     3361 gacgctcgcc gccatctacg cgagccacgg acacgg
//
LOCUS       DASYVO010000059         4885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_129248, whole genome shotgun sequence.
ACCESSION   DASYVO010000059 DASYVO010000000
VERSION     DASYVO010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129248"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            373..1134
                     /locus_tag="VGR03_01975"
     CDS             373..1134
                     /locus_tag="VGR03_01975"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV1993074.1"
                     /translation="MNSLFIAAPSALAAMGAFAYGAVHPRSQLFGRTICRTNSPRKLA
                     ITFDDGPNPSITPKLLDLLDRHNARATFFLIGCYVRECPELVKETVARGHLVGNHTET
                     HPNLFRLTRREIRIELRLCHDAISNALGSPPKWFRPPFGMRNPWVIPATRELGYRTAM
                     WTLIPGDWREKPAEWLIPRMQPIADHAQRNLSKDAHSQWSGEGDVLCLHDGAHRELNG
                     DRSRTLAALEYWLPRWRDLGLEFVTIGEAVTTPAA"
     gene            1137..1451
                     /locus_tag="VGR03_01980"
     CDS             1137..1451
                     /locus_tag="VGR03_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993075.1"
                     /translation="MDERQFYSEKEEVRKVKLVCPSCRGEFEAPVRWKVCRKKKELPR
                     GIGEEDKRKFAKASSYMVRLDDLVACYKCRKRFELTGQSTVLINDNLGDPSADPENFG
                     NR"
     gene            complement(1578..1778)
                     /locus_tag="VGR03_01985"
     CDS             complement(1578..1778)
                     /locus_tag="VGR03_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993076.1"
                     /translation="MAKLALIYGMRLLPEEELGEVKDATVVLKNGRTARVTMHLIEGS
                     KEEIENTFRQSIEAFFEMYPEI"
     gene            1921..2709
                     /locus_tag="VGR03_01990"
     CDS             1921..2709
                     /locus_tag="VGR03_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993077.1"
                     /translation="MREQLRRGIRQNLCFIVGLLAVGASCAAQQPDGPQTAAPSPVAS
                     KPAPAQQAGSNEKILIPAGTRVAVVLQNGISTHSAKPGDSVYLQTSFPITQSSRIVIP
                     VGSYLRGALLESKRPGRVKGRGEFRLRLDTLILPNGYTVSLNAAPRSADSGGKETMDS
                     EGKVTGPGGKGKDAGTIAETTATGAGIGAIARGAKGLGIGAGIGAAAGLAAVLLTRGP
                     DAELPRGSTLDIVLEHDLLLDSSQIQYRNLGQLQPITPPPAQQP"
     gene            complement(2744..3454)
                     /locus_tag="VGR03_01995"
     CDS             complement(2744..3454)
                     /locus_tag="VGR03_01995"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV1993078.1"
                     /translation="MKISKTNAVLIWKQIEDVLVPRLCLTLAERAVYSHLLRHSRLED
                     RLRLRFSILWLARGNRLSAGSVRLAVRRLVAKGALRLVERTKAGHVVEVRLPGEIRAV
                     RRDRIAARGPARLPRAANLEEMDFLQTKPLREAIHSRERSVCFYCLRRLTARVRCLDH
                     VVPQVRRGSNSYRNLVSSCAECNSQKGETPAEGFLRSLYRDGRLTAPELTGRLRAVEA
                     LAAGKLRPGLRSEDAKDD"
     gene            4047..4841
                     /locus_tag="VGR03_02000"
     CDS             4047..4841
                     /locus_tag="VGR03_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993079.1"
                     /translation="MPRVVPSQIVDFINTRWPSADQQKHVGRAQAGHLTGLVDLADQV
                     PGELLTMESALYALFLSAKGHIRHRLVYWATSDPNRGHDLGVMSGQPEQVPVTVIRDA
                     LARCPDESPESATSELTFITDPDLRTNLRIDVGAVTRALSNGEWKAATVLAGSAAEAL
                     LLWALKQRLLPDITSAIATARASGELSSKPDPNDLDRWNLHEYTEVAAKLKIIKPQTA
                     EQTRLARHFRNFIHPGVALRYSEKCDRATALSAVAGMEHVVRDLTP"
BASE COUNT         1066 a         1529 c         1326 g          964 t
ORIGIN      
        1 atcggcgaac cccatagcga tagtccagcg aggaacgtga aaagccagag caaccgagag
       61 ctgaacggtt tctggctaac gggacttttc ctgcctccca atgtggattc ttcacacacg
      121 gcgacactcg cttctacagc aatacgtagt ccattcccct ccacaagttc cctaacttgt
      181 aagatctaag ttgcctaaaa tcctttactt gcaaagtgct tgatttgaaa cgaatgaaaa
      241 gtgtccctcg gaggtacaca cccctgaatt cgaaccaaat tggtacaatc agtgtctcga
      301 ttttcgatac agcttgtcgg atcaggtttt gcagggcatg gattgctgcg atttttgggg
      361 aaggaagtcg gcatgaattc gctgttcatc gctgcgccga gcgcacttgc ggccatgggg
      421 gccttcgctt acggtgccgt tcatccgcgc tcgcagcttt ttgggcgtac gatctgccga
      481 accaattcac cgcgcaagct ggcgatcacg tttgacgacg gaccgaatcc ttcgatcaca
      541 cctaaactgc ttgatctcct cgatcgccac aacgcccgcg cgacattttt cctgatcggg
      601 tgttacgtgc gcgaatgtcc tgaactggtg aaagaaaccg ttgcgcgtgg acacctcgtc
      661 ggcaatcaca ccgaaacgca tccgaatctt ttccggctca cccgcagaga gatcaggatc
      721 gagctgcgcc tctgccacga cgcgatttcc aatgcgctag gctcgccgcc aaagtggttt
      781 cgcccgccct ttgggatgcg taatccctgg gtcattccgg cgacccgcga acttggctac
      841 cgcaccgcca tgtggacgct gatccccggc gattggcgag agaaaccggc ggaatggctg
      901 attccgcgca tgcagcccat cgcagatcac gcccagcgaa acctttcaaa agatgcccat
      961 tcccaatggt caggtgaagg cgacgtgtta tgcctgcacg acggcgcgca tcgcgaatta
     1021 aatggcgacc gctctcggac gctcgccgct cttgaatatt ggctgccgcg ctggcgtgac
     1081 ctcggcctcg aatttgttac aatcggagaa gcggtgacga cacccgccgc ctaactatgg
     1141 atgagcgcca gttttattca gaaaaagaag aagtccggaa agtaaagctg gtctgcccga
     1201 gttgccgcgg cgaattcgag gcgccggtgc gttggaaggt ctgccgcaag aaaaaagaat
     1261 tgccgcgcgg aatcggcgag gaagacaagc gaaagtttgc gaaggccagt tcttacatgg
     1321 tgcggctgga tgacctggta gcttgctaca agtgccgcaa gcgcttcgaa ttgaccggcc
     1381 agtccacggt tctcatcaac gataatctgg gcgatcccag cgccgacccg gaaaatttcg
     1441 gcaatcgctg ataaagctgt cagttctgag caattagttt taagtgactc cgacaaatcc
     1501 gcgagcttca tccgtgttca gagctgctcg atggtacggg ttcaactaac cactagaaac
     1561 taacgactga aaactcctca aatctccgga tacatttcaa aaaaagcttc gatggactgg
     1621 cgaaaggtgt tctcgatctc ttccttgctg ccttcgatta agtgcatggt cacgcgcgcc
     1681 gtacggccat tcttcagcac caccgtcgcg tccttcactt cgccaagctc ttcctcgggc
     1741 agcagcctca tcccgtaaat caacgccagc ttcgccatgc gattctcctc actcttcttt
     1801 aagcgtagcc ctcgttttct cgccggtcaa accccggcga atagatgagc agatggcgtt
     1861 atactcaggc atccaagcta gtcgcttgat gcatcgcatc aagcattgcg gagggttgcc
     1921 gtgagagagc agctgcgtcg agggattcgc caaaatctat gtttcattgt cggcttgctg
     1981 gcagtgggcg catcctgcgc tgcgcagcaa cccgatgggc cgcagactgc cgccccctca
     2041 ccagtcgcat ccaagcccgc tccggctcaa caagctggct ccaacgaaaa gattctgatc
     2101 cctgcgggaa cgcgcgtcgc cgttgtgctc cagaacggta tctccaccca tagcgccaag
     2161 cccggagact cggtttacct tcaaacttcg tttccgatca cgcagagcag tcgcattgtg
     2221 atcccggtcg gttcctatct tcgcggtgcg ttgctggaat cgaagcggcc cgggcgcgtc
     2281 aagggccgcg gtgaattccg gctgcgcctg gataccttga tcctgccgaa cggctacacc
     2341 gtcagtttga acgccgcgcc acgcagcgcc gattccggcg ggaaagaaac catggattcc
     2401 gaaggcaaag tcaccggccc tggcggcaaa gggaaggacg ccggcacgat cgcggagacg
     2461 accgccactg gcgcggggat cggggcaatt gcccgcggag cgaagggact tggtattggc
     2521 gcggggattg gcgcggccgc ggggttggcc gctgtgcttt tgactcgcgg gccggacgcc
     2581 gaactcccac gcggctccac gctggacatc gtactcgaac acgatctttt gctcgacagc
     2641 agccaaattc agtacaggaa tctcgggcag ctccagccca ttacgcctcc tcccgcgcag
     2701 cagccctagc cttaattcgc tttgccccac cacactaagg atgtcagtcg tcctttgcgt
     2761 cctcactgcg caacccgggc cggagcttgc ctgccgcaag cgcttcgacc gcgcgcagcc
     2821 gtccagtgag ttcgggggcc gtcaaacgcc cgtcgcggta gagcgagcgc agaaaaccct
     2881 ctgctggggt ttcccctttc tgcgagttgc attctgcgca ggaggaaacc aggttgcggt
     2941 aggaattgct gcccctccgg acttgcggca cgacatgatc gaggcagcgc acccgggcgg
     3001 tgagccggcg caaacaatag aagcacacgc tgcgttcgcg cgaatgaata gcctcccgca
     3061 gcggcttcgt ttgcaggaaa tccatctcct cgaggttggc ggctcggggc agccgcgccg
     3121 ggccgcgggc cgcgattcta tcgcgacgga cggcgcgaat ctccccgggc agccggacct
     3181 cgacgacgtg gccggctttc gtgcgttcga ccaggcgcag agcccccttg gccaccaggc
     3241 gccgcaccgc caggcgcacc gacccggcag acaggcggtt gccgcgggca agccaaagaa
     3301 tcgaaaagcg cagccgcagc cggtcttcga gccggctatg ccgcagcaga tgagaataga
     3361 ccgcgcgttc cgcgagagtg aggcacagcc gcggcacaag aacatcttcg atttgtttcc
     3421 agatgagcac agcattggtt ttgctgattt tcatggtaat ggtccttaca agtccaaagt
     3481 ttcgtcagtt cacagtttct cgagggcaga gtttcgtcag ggcacgactt tagtcgtgcc
     3541 ataggcgctt cgtttctgaa aaaatctcct acctgtcatt ccgagcgcag cgagacctct
     3601 ccctcctgtc attccgagcg cagcgaggaa tctgcttttc gtcttccttg cttgccatca
     3661 ggccacacga gtgcgcgagc ctactcccgc cgccccgcac gccggggaac gaaagtgcgc
     3721 tgcaactttt atgtaatttg ggaaaaggaa tgaagggtaa aaacaaaaag ggcgaaaccg
     3781 tccaccgaca gtttcaccca gaatcagtat accacatcta aaatggaatg caagcactat
     3841 ttagcaagtt ggttagtttt cgattattgt gtccggttag ccccgcactc ctccgcctca
     3901 tcacgcgcct cgcgatgcgc cccacccaga tcgaccgtcc gacgaagtca cggtgagatt
     3961 ccgagcaccc gccggaatcg ataaggacac cgcaccgcta gccaccagaa caccgctctg
     4021 gtagaactgc attctagagg tgtccaatgc cacgagtcgt ccccagccaa atcgttgact
     4081 tcatcaacac ccgctggccc tccgccgacc aacaaaaaca tgtcggcagg gcacaagccg
     4141 gtcaccttac cggtctcgtt gatttagccg accaagttcc gggcgaactc ctcaccatgg
     4201 agtccgccct ctacgcactg ttcctctccg ccaaaggtca catcaggcac aggctggtat
     4261 actgggccac cagcgaccct aaccgcggac acgacctcgg cgtcatgtcc ggccaacccg
     4321 agcaggttcc agtcacagta atccgcgacg cgctcgcgcg gtgtccagac gagtcccccg
     4381 agtctgccac atcggaactc accttcataa ccgacccgga cctccgaacg aacctacgca
     4441 tcgatgtcgg cgcagtcacc agagcgctct cgaacgggga atggaaggcg gcgacggtcc
     4501 tcgctggctc cgcagccgag gccttgctgc tgtgggcact caagcaacgt ctactcccag
     4561 acatcacgag tgccatcgcg actgccaggg cgagcggtga attaagctcg aaaccagacc
     4621 cgaatgatct agaccgctgg aatctgcacg agtacaccga ggttgccgca aagctcaaaa
     4681 tcatcaagcc acaaaccgca gagcaaacaa ggctggcgag acacttccga aacttcatcc
     4741 atcccggcgt cgctctacgc tacagtgaga aatgcgaccg cgccacggcc ctctcagccg
     4801 tcgccggaat ggagcatgtc gtgagagacc tcaccccgta aaagccctgc aactgcaagc
     4861 gagccgccag cgttgcgtcc acttg
//
LOCUS       DASYVO010000060         7090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_85869, whole genome shotgun sequence.
ACCESSION   DASYVO010000060 DASYVO010000000
VERSION     DASYVO010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85869"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2456)
                     /locus_tag="VGR03_02005"
     CDS             complement(<1..2456)
                     /locus_tag="VGR03_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1993080.1"
                     /translation="MDAAKTESLHKRIFRFLVRLFPLEFRWDYGRDMEHVFEDQRREA
                     ASRGGIMGILRLWWETLAGIFTTAPREHWEMLKQDGGYALRIMRKNPGFTAVVVLTLA
                     LGIGANTAIFSVINGVLLRPLPYAQGDQLIRLRQNATRAGISDVAFSVKEIADYHEQN
                     QTLSGTAEYHSMSFTLFGQGEAQRLLIGVVSANYFDVMGVKPILGRLFLPGEDRVGAD
                     PVLLLTYGYWKNVMGGDPNVVGKTFEMNDRVHTVIGVLPPLPDYPDANQMFMPSSSCP
                     FRSNPRTVENRQARMLSLFARRNPGVPVAQVSADLSTIAHRLEIQYPGDYPKADGYDV
                     DELPIQSEMTGGARTTFLILLGTAGLVLLLTCANVANLTLSRQIRREHELAIRATLGA
                     SRARVFRQLLTESTLLAVAGGGLGLLVAAGGISLLVAFAARLTPRTGEIGIDSYVLLF
                     TLAISVLTGVLFGSIPAFSSRQDLAGVMNQGGGRTSGSVSKHRVRNMLVTAQVAISFI
                     LLIGAGLMLRTLVKLQEVDPGFRPQNVLSSTVSLNFTKYRDQKLSLAFYENVLDNLTQ
                     RPGVQSAAVSLTVPLGNQSGPNDGQVVIEGRPLPAGTPAPKVDFRVVSPNYFRTMGIT
                     QIRGRDFTSDDKADRPPVAIVNQSMARHNWGDADPVGQRASADGGKTWITIVGVVGDV
                     KQYGLNRVATDEFYVPLESNSLLQAAILIRTAINPLLMNKEIVKAVHEIDPQQPVIRV
                     MTLEQIRNEWLASPRLTAILITLFAGLALVITLAGISGVMALAVSQRTREIGIRMALG
                     ASKATVLMMVLRQGMILVVLG"
     gene            complement(2505..2873)
                     /locus_tag="VGR03_02010"
     CDS             complement(2505..2873)
                     /locus_tag="VGR03_02010"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1993081.1"
                     /translation="MADKKRDTSPHLPLTPAEFHLLLALADGDKHGYAIMKEVSRRTD
                     DKVRLSAGTLYGLIRRLVSEGMIVETEERPVAALDDERRRYYRLTQFGRQVAIAEAQR
                     LEGVLELARVKKLLVRPRTA"
     gene            complement(3833..4399)
                     /locus_tag="VGR03_02015"
     CDS             complement(3833..4399)
                     /locus_tag="VGR03_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993082.1"
                     /translation="MIAPARVEIIQTLRPAVSREEALAKLRPAGLPGLLRSALRGPLR
                     RIADAYVPYRLYRVEAEGVPRFGTQYMALDAVQGTLDPYRFEGFPVGPSLVSLETRNR
                     LPRILEEERTRELLIESVRRWVYQRGFFRLRRLEIRLELVPLDLHIPYWLAFYGTGER
                     AQISVLDAVRRQVEGARARDLFRRWLLT"
     gene            complement(4396..6090)
                     /locus_tag="VGR03_02020"
     CDS             complement(4396..6090)
                     /locus_tag="VGR03_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEV1993083.1"
                     /translation="MYLMASSAVTHRVLESLDLAVIAVYFLVVFSIGLYFARKERTSE
                     DYFLAGRNVGWFAIGASLFVSNISTEHFIGLAGSGATSGLAVGHFEWLACLILLVLGW
                     VFVPFYLRSNVFTMPEFLERRFNRSCAVYLASISILAYIFTKISVHLYAAAVVLERVV
                     GWNPLTAAILLVVATGVYTIAGGLAAVIYTDLVQTLILIGGAVILTVIGMREVGGFTG
                     LRAALPANYFHMIKPMHDAEFPWTGIFLGAPILGIWYWCTDQVIVQRVLSAKDEGHAK
                     AATIFAGFLKILPVFILVLPGLIAAALYPQLFTRGADGTISNGDIAYPSLIINLLPHG
                     LVGLMIAALMAALMGAMSSVFNSASTLVTLDFYKKIRPAATEHQLVTFGRAATGILVV
                     LGLLWVPFVKLISAQLFIYLQSVQAYISPPIAVCFIFGILWPRMNGTGAISSLLSGFV
                     LGALRFVLEVLDKSGHFQVAAIRWLLDINFLHYAILMFVACSVVLIAVSLMTVASTPE
                     KLAGLTFATVDLKLDGVAPVGHRVHLARETAFERNMNIAFSALLIATVVGLWIYFR"
     gene            6367..>7090
                     /locus_tag="VGR03_02025"
     CDS             6367..>7090
                     /locus_tag="VGR03_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993084.1"
                     /translation="MQEDALLRELSPETRPRAGITRREWLGRAAGLICLSPLAALHGT
                     GAMRALLGQALVTQAQPAFKFSVEEEAFLDEVEKACSLFFWEQANPYTGLVKDRSQAN
                     GPDARDTASIASTGFGLTALCIADQRGYLPAAKARSRVRDALHFLWKGMRHEHGFYYH
                     WVNMHNGERVSESEVSSIDTALLLCGVLTCGQHFEDQEIRSLTAQIYERVDWPWMLNG
                     GKTLSHGWKPETGFLKPRWDTYN"
BASE COUNT         1487 a         2156 c         2163 g         1284 t
ORIGIN      
        1 ccgagcacga cgagaatcat gccctggcgc aggaccatca tcagcaccgt ggcctttgag
       61 gcgccgaggg ccatgcggat gccgatctcg cgcgtgcgct ggcttaccgc cagcgccatg
      121 acaccgctga tccccgcgag cgtgatcacc agcgcgagcc cggcgaacag cgtgatgagg
      181 atcgccgtca gcctcggcga cgccagccac tcgttgcgaa tctgttcgag cgtcatcacg
      241 cgaatcaccg gctgttgcgg gtcgatctca tgcaccgcct tgacgatctc cttgttcatc
      301 agcaacggat tgatcgccgt gcggatcagg atggccgcct gaagcaggga gttggattcg
      361 agaggcacat agaactcatc ggtcgcgacg cggttcaacc cgtactgctt gacgtcgccg
      421 acgacgccga cgatcgtaat ccacgtcttt cctccgtcgg ctgaagctcg ttggccgaca
      481 gggtcagcgt cgccccagtt gtgccgggcc atggactggt tgacgatggc caccggtggc
      541 cggtcggcct tgtcgtcgct ggtgaagtcc cgcccccgaa tttgcgtgat tcccatggtg
      601 cggaaataat tcgggctgac cacgcggaaa tccactttcg gagcgggagt acccgccggc
      661 agaggacgtc cttcgatgac cacctgcccg tcattcggtc ccgactggtt gcccagcggc
      721 acggtcaggg aaacggctgc actctggacg cccggccgct gggtcaaatt gtcgaggaca
      781 ttttcgtaga aggcgaggga tagcttttgg tccctatact tggtgaagtt aaggctcacg
      841 gtgctgctca acacgttttg ggggcgaaaa cccgggtcga cctcctgcag cttgaccaga
      901 gtgcgaagca tcagccccgc gccgatcagc aggatgaagg aaatcgccac ctgcgccgtc
      961 accagcatat tgcggacgcg atgcttgctc acactgccgc tggtccggcc gcctccctgg
     1021 ttcatcaccc cggccagatc ctgccgggag gagaacgcgg ggatcgaacc gaacagaacg
     1081 ccggtgagta ccgaaatggc cagcgtgaaa agcagaacgt aggaatcgat gccgatttct
     1141 ccggtccgcg gcgtgagccg cgccgcgaac gccaccagca gactgatgcc tccggccgcg
     1201 accagcaggc cgagaccgcc gcccgccacc gccagcagcg tgctctccgt cagcagttgc
     1261 cggaagacgc gggcgcggct cgcgccgagc gtcgcgcgga tggccagctc gtgctcccgc
     1321 cgtatctggc gggacaacgt aagattggcc acgttggcgc aggtcagcag cagcaccagc
     1381 cctgccgtcc ccagcagaat cagaaaggtc gttcgtgccc cgcccgtcat ttccgactga
     1441 atcggcaact catccacatc gtagccatcc gccttggggt aatcgcccgg gtactggatc
     1501 tcgaggcgat gcgcgattgt gctgagatca gcggagacct gcgcgaccgg cacgcccgga
     1561 tttcgccgcg cgaagaggct caacatcctg gcctggcggt tttcgacggt cctggggttt
     1621 gaccgaaacg ggcaggagga ggacggcatg aacatctgat tcgcgtccgg ataatcggga
     1681 agtggcggaa gaacgccgat caccgtatgc acgcggtcgt tcatttcgaa ggtcttgccc
     1741 accacgttcg gatcgccgcc cattacgttt ttccagtagc cgtacgtgag cagcagcacc
     1801 gggtcggcgc cgacgcgatc ctcgccggga agaaacaacc ggcccaggat gggcttcact
     1861 cccatgacgt cgaaatagtt cgcggagacg acgccgatca gcaagcgctg cgcctcgccc
     1921 tggccgaaca gcgtgaagga catcgagtgg tactcggcgg tgccagagag cgtctggttc
     1981 tgctcgtggt agtcggcgat ctccttcacc gagaaggcca catccgaaat gccggcgcgc
     2041 gtcgcattct ggcgcaagcg gatcaactgg tcgccttgtg cgtagggcag cgggcgaagc
     2101 agtacgccgt tgatgacact gaaaatcgcc gtgttcgcgc cgatgcccag cgccagcgtc
     2161 agcacgacca cggcggtgaa gcccggattc ttgcgcatga tgcgcaacgc gtagccgccg
     2221 tcctgtttca gcatctccca gtgctcgcgc ggcgccgtcg taaaaattcc cgccagtgtt
     2281 tcccaccaca atctaagaat gcccatgatg cctccccggc ttgcggcttc gcgccgctga
     2341 tcctcaaaga cgtgttccat gtcgcgccca tagtcccagc gaaactcgag cgggaacagg
     2401 cgcaccagga aacgaaaaat gcgcttatgc agcgactccg ttttagcagc gtccatgatg
     2461 cttctcctcg gctccgtgcc gcacgtttga cggtccgcgg ccgcctaggc agttcgcggg
     2521 cgaacgagca gtttcttgac gcgcgccagc tccagcacgc cctcgaggcg ctgggcctcg
     2581 gcgatggcca cttgccgccc gaactgcgtc agccggtaat atcgccggcg ctcgtcgtcg
     2641 agcgcagcca ccggccgctc ctcggtctcg acgatcatgc cctcgctcac caggcggcgg
     2701 atgaggccgt agagcgtgcc cgcgctcagc cgcaccttgt cgtccgtgcg ccgggagact
     2761 tccttcatga tggcgtagcc atgcttgtcg ccgtcggcca gcgccagcag aaggtggaac
     2821 tctgccgggg tgagcggcag gtgcggtgag gtgtcgcgct tcttgtcagc catgaattcc
     2881 tctcggccgc gcggggaccg cgccgcctct ctatcagacg taaagggact atatcgcgtt
     2941 tcgctatagt gtcaaatttt ttcgaggacg ggccgaatgg cttggaggag cccacacgaa
     3001 aggcagaagg gagtagcgcg catgcggagc ggattctgga accctgccca tcgcccaagt
     3061 cgtgttcaag gacgccctgg acaagctgca aactcgaggc ttcagcgggt accacctgca
     3121 agagcacgtc gccgtaggta ggagggcaga gagagcactt tgtcagattc cccagggcaa
     3181 acgcggcgaa gcacatggcc agccctagtt ggctcagcgc ggccccaaaa tactgcctaa
     3241 tttctgtggg tacgccgctt gcgggcggtc tgggatgatc gcggatcccg ctaggattgc
     3301 cgccatgcgc ttccttgcgc ctcctggcgg gtcgtgtacc ccgaaacggg cctagccaag
     3361 gttgcagcgc agcgcgccat tgctagcttg cccaaaatcc tccagctgcc cacaatcctc
     3421 cgaaaatcgg ctaccggaaa tcctatgtgc atgcttgccc ataaagaagt gattttgggc
     3481 agggttgcaa ttgtgagcct tcgtggatct gaacctccga ccccctggtc ccgaaccaga
     3541 ttctaactct tgttgaagtc catagaattc tgttgctctt agttgattga tgatgaagcg
     3601 cttgcggtgg acttgttgaa attgattgaa ccttatggaa gttggctgtt cttgcagcta
     3661 caaaatcgtc tacagcgagg attccttcga gcgaacgaac ccgctcgtaa attgcgaaag
     3721 ccaagcgaaa aagaaatcgg ggatggtcgt ggcgtccgtc ttgagttttg acggaggaca
     3781 gggaaggatt ctcggcgtca ttgtttcccg tgaagtcatg ccaagaactt atctaagtca
     3841 gaagccagcg acggaagagg tcgcgcgccc tggcgccttc cacctgccgt cgtacggcat
     3901 cgagcacgga gatttgcgcg cgctcgcccg tcccgtaaaa ggccagccag tagggtatat
     3961 gtaaatcaag aggtacgagc tcgaggcgga tctcgagacg ccgcaggcgg aaaaatccac
     4021 gctggtacac ccaccggcgc acgctctcga tgagcaattc ccgagtgcgc tcctcctcca
     4081 gaattcgcgg caggcggttt cttgtttcga gcgagaccag cgaaggccca acgggaaagc
     4141 cctcgaatcg gtatggatcc agcgtgccct ggacggcatc cagcgccatg tactgggtcc
     4201 cgaaccgcgg gacaccctca gcctcgacgc gatagagccg atagggtacg taggcgtcag
     4261 caatgcggcg caacgggccg cggagtgcgc ttctcagcag acccggcaag ccagcggggc
     4321 gcagttttgc aagcgcttct tcgcgagaca ctgccgggcg aagcgtctgg atgatctcca
     4381 ctctggcagg cgcgatcatc gaaaatagat ccacagaccg acgaccgtgg cgatgagcag
     4441 cgcgctgaaa gcgatattca tgttccgctc gaacgccgtc tctctcgcaa gatgcactct
     4501 gtggcccacc ggggcgacgc catcgagctt caagtccacg gtggcgaagg tcagcccggc
     4561 aagtttctcg ggcgttgacg cgaccgtcat caggctcacg gcaatcaaaa ccacactgca
     4621 cgcgacgaac atcagaatgg cgtaatgcag gaaattgatg tccagcagcc agcggatggc
     4681 ggcgacctgg aaatggccgc ttttgtccag cacttcaagg acgaagcgca gcgcccccag
     4741 gacgaatccc gagagcagtg aagagatcgc tcccgtgcca ttcatccgcg gccagagaat
     4801 gccgaagatg aagcaaacgg cgatgggcgg gctgatgtat gcctgtacgc tttgaagata
     4861 gatgaaaagc tgagcgctga tcaatttgac gaacggcacc cagagcagcc cgaggaccac
     4921 aagaatgccc gtggccgcgc gaccaaaggt gaccagttgg tgctcggtcg cggcgggccg
     4981 gatctttttg taaaagtcga gcgtcaccag agtcgacgcg gagttaaaca ccgaactcat
     5041 cgcgcccatc agcgcggcca tcagcgcggc gatcatcagc cccaccagcc cgtgcggcag
     5101 aagattgatg atcagcgagg gataagcaat gtctccgttg ctgatcgtgc cgtcggcccc
     5161 gcgggtgaaa agctggggat agagggcagc ggcgatcaag ccgggcagca ccaggatgaa
     5221 caccggaagg attttcaaga aaccggcgaa gatcgttgcg gccttggcgt gtccttcatc
     5281 tttcgcagag aggacgcgtt ggacgatgac ctggtcggtg caccaatacc agatgccgag
     5341 aatgggagcg cccaaaaaga tcccggtcca gggaaactcg gcatcgtgca tcggcttgat
     5401 catatggaaa tagtttgccg ggagcgcggc tcgcaagcct gtgaagccgc cgacttctcg
     5461 catgccaatc accgtgagga tgacggcacc accgatcagg atgagcgtct gcaccaggtc
     5521 tgtgtaaatg accgccgcca gtcctccggc gatggtgtac acgcccgtgg cgacgaccag
     5581 caggatggct gcggtcaacg gattccagcc cacgacgcgc tcaagaacca ctgccgcggc
     5641 gtacagatgg acggaaattt ttgtgaagat gtacgccagg atggaaatcg aagccagata
     5701 gaccgcgcag gagcggttga accgccgctc cagaaactcc ggcatagtga aaacgttgct
     5761 ccgcaggtaa aaagggacga aaacccagcc aagcaccaac aggatcaaac acgccagcca
     5821 ctcgaagtgg ccgacggcca gcccggaggt ggcgcccgat ccggccagtc cgatgaagtg
     5881 ttcggtcgaa atgttggaaa caaagagcga agctccaatc gcgaaccaac ccacattccg
     5941 gccggccagg aagtagtcct ccgaggtgcg ctccttgcgg gcaaagtaca aaccaatcga
     6001 gaaaacaacc agaaagtaga cggcgatgac cgccagatcg agcgactcga gcacgcggtg
     6061 cgtaacggct gaggatgcca tcaggtacat cgcagtctcc tgtcgatatc cgattggatt
     6121 ggtgaacgcc gctcgttttc cccgggacgt agaggcgtgc ttcggtgcag gatcagtgcc
     6181 tgaagaacgc cacaacggag ggcaccatac cactccccgg gcttcttttc acggggaaag
     6241 aaaccctggt tacgctagta aatcgatttt gttcatgaga ggggccggga aatcctccct
     6301 tcacgctttc cccggctcgc tttcttggcg ataatgaata atggagtgtc cacccatgag
     6361 ggaatagtgc aagaagatgc tctgctgagg gagttgagcc ccgaaactcg tccgcgcgcg
     6421 ggcatcaccc ggcgagaatg gctggggcgc gccgccggct tgatctgcct ctcccctctg
     6481 gctgccctac acggaacagg agccatgcgc gccctgctcg ggcaggctct agtcacgcag
     6541 gcgcagccgg ccttcaaatt ctctgtcgag gaagaggcgt ttctggacga agtggagaag
     6601 gcctgctcgc tctttttctg ggaacaggcc aacccgtata caggcctggt gaaggaccgc
     6661 agccaggcga atggccctga cgcaagggac acagccagca ttgcctctac gggtttcggt
     6721 cttacggctc tttgtatcgc agaccaacgc ggctatcttc cggccgcgaa ggcgcgttcg
     6781 cgcgtccggg acgcccttca ttttctctgg aaggggatgc ggcatgagca cggcttctac
     6841 taccactggg tgaatatgca taacggggag cgcgtctcgg agtcggaagt ctcctcgatt
     6901 gacacggcac tcctgctgtg cggcgttctc acctgcggcc agcatttcga agaccaggag
     6961 atccgcagcc tcaccgccca aatttacgaa cgcgtggatt ggccatggat gctcaatggc
     7021 ggcaagacgc tctcgcacgg ctggaaaccg gagaccggat ttctgaagcc gcgctgggac
     7081 acctacaacg
//
LOCUS       DASYVO010000061         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_129311, whole genome shotgun sequence.
ACCESSION   DASYVO010000061 DASYVO010000000
VERSION     DASYVO010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129311"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VGR03_02030"
     CDS             <1..352
                     /locus_tag="VGR03_02030"
                     /inference="COORDINATES: protein motif:HMM:NF037139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993085.1"
                     /translation="IFFVDLPAAGERRAIFKVHLLKKDRRPEDFDLEALAEAAEGFSG
                     AEIEQAIKGALYTVFSAHQELSTAAILAEMKATVPLSSTRREDIEALRRWARGRAVRA
                     NQEAAEESEESIRR"
     gene            547..855
                     /locus_tag="VGR03_02035"
     CDS             547..855
                     /locus_tag="VGR03_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1993086.1"
                     /translation="MRKPRKHYTAEEKVAILRRHLLEHEPISKLCEEHGLQPAMFYRW
                     QKEFFENGAAAFQSKARTNQGAEQERIEFLEKRIQRKDEVLAELMEEHVALKKSLGEP
                     "
     gene            867..1808
                     /locus_tag="VGR03_02040"
     CDS             867..1808
                     /locus_tag="VGR03_02040"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV1993087.1"
                     /translation="MPPDVRDQVVDFVRRWSEKTEMGAGRIVGWLQVAASKFYNWRKR
                     YGRVNEHNGWVPRDFWLEPWEKQAILDYQEKFPLEGYRRLTFMMLDADVVAVSPSSVW
                     RVLRQAGRLRKWQPKPSRKGTGFEQPLLPHLHWHVDVSYINVAGTFYYLCSVLDGYSR
                     SIVHWDLRESMREAEIEIILQAAREKYPEAKPRIISDNGPQFMARDFKEFIRICGMTH
                     VRTSPYYPQSNGKLERFHQSLKSECIRPGTPLNAEDARRLIQRYVDHYNTVRLHSAIG
                     FVTPQDMLAGRQAEIHAARDRKLEAARQQRQLRQQAA"
     gene            2065..>2567
                     /locus_tag="VGR03_02045"
     CDS             2065..>2567
                     /locus_tag="VGR03_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEV1993088.1"
                     /translation="MRSIEYDTVDDTYPRFLRDEILADVAKEYNIRRDSYSRAIAGES
                     SGGICSFNAAWQQPGEFSRVLSSIGSFTSIQWRFGNSDLTKNLEGGNVYPFKIRKEPK
                     RNIRVWLSDGSSDLENDHGSWPLQNIQMANSLKYKEYDFHFSFGIGTHNGAHRNSELP
                     EALTWLWR"
BASE COUNT          573 a          751 c          798 g          445 t
ORIGIN      
        1 gattttcttc gtggacctgc ccgcggccgg ggagcgccgc gcgatcttca aggtgcacct
       61 gctcaaaaag gaccgccgcc cggaagactt cgacctggag gccctggccg aggccgccga
      121 aggcttcagc ggcgcggaaa tcgagcaggc catcaaaggg gccctctaca cggtcttttc
      181 ggcccaccag gagctttcta ctgccgccat cctggccgag atgaaggcca ccgtgccgct
      241 ttcctccacc cgccgtgaag atattgaagc cctgcgccgc tgggcccgcg gccgcgccgt
      301 ccgcgccaac caggaagcgg cggaggaatc agaggaatcc atccgcagat gacgcagatg
      361 acacagattc ccggttctgc gtcatccgcg tcatctgcgg attaggattt tgtctttgtt
      421 ttgtgctaag agattgaaac ccggaaggga cacaagagat gaagcgctat ggcacggagc
      481 taagaatgtc ccgcctcagt tggaaatgga ggaagcgttc tgccgttaag ataacggaga
      541 ggacgcatga ggaaaccacg gaagcactac acggccgagg agaaggtggc cattctgaga
      601 cggcatttgc tggagcacga gccgatctcg aaactttgtg aggaacacgg gctgcagccg
      661 gcgatgttct accggtggca aaaggagttc ttcgagaacg gcgctgccgc tttccaaagc
      721 aaagcccgga ccaaccaggg cgcggaacag gaacgcatcg agttcctgga gaagaggatc
      781 cagcgcaagg acgaggtcct ggccgagctg atggaagagc acgtggccct aaaaaaaagt
      841 cttggggaac cctgaaggat gcctgggtgc cgcctgacgt gcgggaccaa gtggtggatt
      901 tcgtccggcg ctggtcggag aagactgaga tgggcgccgg gcgaatcgtt ggctggctgc
      961 aggtggctgc cagcaagttc tacaactggc ggaagcgcta cggccgggtc aacgaacaca
     1021 acggatgggt tccccgcgac ttctggctgg agccgtggga aaagcaagcg atcctggact
     1081 atcaggaaaa gtttccgctg gaaggctacc ggaggctgac gttcatgatg ctggatgcgg
     1141 atgtagtggc ggtgagtccg tcgagcgtgt ggcgggtgct gcgccaggcc ggacggctgc
     1201 gcaagtggca accgaagccg tcgcgcaagg gcactggttt cgagcagccg ctcttgccgc
     1261 atctgcattg gcacgtggat gtttcctaca tcaacgtggc cggcacattc tactacctgt
     1321 gcagcgtgct ggacggttac agccgctcga tcgtgcattg ggacttgcgc gagtcgatga
     1381 gggaggcgga gatcgaaatc attctgcaag cggctcgcga aaagtatccc gaggccaagc
     1441 cgcggatcat ctccgacaac gggccccagt ttatggcccg cgacttcaag gagttcatcc
     1501 gcatctgcgg catgacccac gtgcgcacct ccccgtacta tccgcaatcg aacggaaagt
     1561 tggaacggtt ccaccaatcg ctgaagagcg aatgtatccg gcccggcaca ccgttgaatg
     1621 ccgaggacgc ccggcggctg attcagcgct acgtggatca ctacaacacg gtgcgcctgc
     1681 atagcgccat cggctttgtc acgccgcagg acatgctggc cgggcgccag gcggagattc
     1741 acgccgcccg cgatcgcaag ctggaagcag cgcggcagca acgccaactc cgtcaacagg
     1801 cggcttgaaa ccatgaccaa tcgagctaca atgactgcgc caggtgaaac ggaagcgggc
     1861 tctgcaggga tgcaaccatg ccgagggata gcctggcgga gactcatcga aaacgatgga
     1921 gggggacggc cttactcgtc gtcctccatc tcaaacatcg gattcgatag atctcctcat
     1981 gccttgaaaa tccccgctcc gaaggggcgg aatctttcac taacggagaa cgctcgttct
     2041 ccatttccgg ctgaaccggg acacatgcgc agcatcgagt acgacacggt ggacgacacc
     2101 tacccgcgct tcctgcgcga cgagattctc gcggacgttg cgaaggagta caacatccgc
     2161 cgcgactcct acagccgggc gattgccgga gagtcgtcgg gcggcatctg ctcgttcaac
     2221 gccgcctggc agcagcccgg cgagttcagc cgcgtgctct ccagtatcgg gagtttcacc
     2281 agcatccagt ggcgtttcgg caattccgac ctgacgaaga acctggaggg cggcaacgtc
     2341 tatccgttca agattcgcaa agaaccgaag cgcaacatcc gcgtgtggct cagcgacgga
     2401 tcgagcgacc tcgaaaacga tcacggaagc tggccgctgc agaacatcca gatggccaat
     2461 tcactcaagt acaaggaata cgatttccac ttcagcttcg gcattggcac gcacaacggc
     2521 gcgcatagaa actcggaact gcccgaagca ctcacctggc tgtggcg
//
LOCUS       DASYVO010000062         8827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_157359, whole genome shotgun sequence.
ACCESSION   DASYVO010000062 DASYVO010000000
VERSION     DASYVO010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8827
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157359"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..480)
                     /locus_tag="VGR03_02050"
     CDS             complement(175..480)
                     /locus_tag="VGR03_02050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1993089.1"
                     /translation="MHIPRHLGGNLVLIGDGPDRGAAEYLVRKKKLQKGVFFLGKQDT
                     VYERLAAADLFLMPSQLESFGLAALEAMACEVPVIATNVGGVPEVVEHGVDGFLAGC"
     gene            complement(506..895)
                     /locus_tag="VGR03_02055"
     CDS             complement(506..895)
                     /locus_tag="VGR03_02055"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV1993090.1"
                     /translation="MESATQPVSKTSLWAGRIISTLVVLFLLFDAIAKLMKIAPVLAA
                     FTQLGFSNSLVVPIGAVLLLCTIVYVIPPTSILGAILLTAYLGGATVTHVRAGQPFYF
                     PIIFGVLVWAGLYLRENRLRALIPLRS"
     gene            complement(986..1282)
                     /locus_tag="VGR03_02060"
     CDS             complement(986..1282)
                     /locus_tag="VGR03_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEV1993091.1"
                     /translation="MIRVILPGHLRTLARVSSEVQLEVVGQVTQRSVLDALEAQYPML
                     RGTIRDHVTQERRPFLRFFACAEDYSLQPADTPLPDAVASGAEPLLIIGAIAGG"
     gene            complement(1279..2463)
                     /locus_tag="VGR03_02065"
     CDS             complement(1279..2463)
                     /locus_tag="VGR03_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993092.1"
                     /translation="MSKVRVLVGTRKGAFILTSDGKRERWDVSGPHFAGWEMYHLKGS
                     PVDPDRLFASQCSGWFGQIIQRSDDGGKTWTQPGTPPGERTTPEGMPKGESNKFAYDS
                     SSETGKPLTTHQWYDGTQHPWEFKRVWHLEPSPTDPDTVYAGVEDAAIFRSTDGGQNW
                     QELAGLRGHGSGPRWAPGAGGMCLHTIILDPSDPQRIFIAISAAGAFRTDDAGKTWRL
                     INKGLHSQHIPEPTAEVGHCVHHVAMHPSRPGVLFMQKHWDVMRSDNAGDSWQEVSGN
                     LPTDFGFVIDVHAHEPETIYVVPIKSDGEHFPPDGKLRVYRSRTGGNEWEALTKGLPQ
                     DNCYVNVLRDAMAVDTLDKCGIYFGTTGGQVYASADAGDSWTPIVSHLPGVLSVEVQT
                     LR"
     gene            complement(2567..5050)
                     /locus_tag="VGR03_02070"
     CDS             complement(2567..5050)
                     /locus_tag="VGR03_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HEV1993093.1"
                     /translation="MNFKSELFLHDLRVALRSLVRTKGLAVTVVLTLALGIGANAAIF
                     SLVRGVLLRPLVNSDEDRLIYIQQSAPGISPGEVPFSVPEIQDLRGGVKTLSSFGEFS
                     TVGFTMIGLGEPREVRAGVVDGSYFEVVGLHPVLGRLIGPQDDGPKAAGVVVLTYRFW
                     STTFKNDPSVIGKTVRLGSGFGDRSATIIGVLEPSIPYPSDTEIIANIVTSPHHLSAT
                     MQNGRVHRMTELFGRLAPGATLDQARAELRTVYGAMIKDHLDAYSPQAHFQIDSKLLR
                     EQITSGARTVLLVLLAASGLVFIIACSNVANLILARTVRREGELAIRAAMGASAGALR
                     RTLLAESLILCVAGATLGVISARPLLAVLARYASRFSVRALDLAVDSSMLWVGALLAV
                     IAAVLLAYVPHLPTANASQTPGLTSGGVRITGSTSRRLRIFAVTQIAASFMLLAGASM
                     LLKTLLAIQSAQTGLDTRHVLAINVPAVSHGKTPEQVVDFYKESMRRIDALPGVNKTA
                     FGIVVPWRDAGSFGPGFQFSADGHVRGPGEEDPRAQLRTISPGFFAALGVPIIAGRDF
                     NQLDGQTKDPVVIVSQTLAQRMFPNQDAVNRHVYWTDPVMKFIPGISTAPHRIIGVTA
                     DIDDEHVVPGPSLTVYSPFEEGPIFGGRLFIHTSANPYSLVTPITRIIRDMSVDQPVE
                     RAATLEDVRAEVLTPDRLNTVVFGVFAAVALAIALVGVAGVLAFSVSARTREFGVRLA
                     IGAEPRHLLTGVITEGAVMAVVGIVAGAACGYALARLAGSYFQDVRMPSALPVVGSVV
                     VLLAAAVIASAWPAARAARINVIEALRSE"
     gene            5021..5578
                     /locus_tag="VGR03_02075"
     CDS             5021..5578
                     /locus_tag="VGR03_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1993094.1"
                     /translation="MKEQLGFEIHLPLSLSHLENECQSADFTCEMQVKICYCGNMPKK
                     KASPKWRSGCPLNASVEILGDRWSLLIIRDMMLRGFRSYKEFLESYEGIATNILADRL
                     QRLIAHGIITTERDPLDGRKQNYSLTEKGIDLAPVLTEMVLWAAGHEDTDNQALVQLI
                     KKDKEQFLAAARQRWAEKTIRSTAR"
     gene            complement(5618..6613)
                     /locus_tag="VGR03_02080"
     CDS             complement(5618..6613)
                     /locus_tag="VGR03_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993095.1"
                     /translation="MDPAPSLPNAATAASNPAAPTKADKRSALYDLIAFFLEGGIQIW
                     IRITGKRIREADAPWLRCPLGKRGRIGTRIYERIARDENLQIQVPPSAGLIPDFNALR
                     GPGFNPDAIHPAIRHFYEHAAAYHLEVWTEVSLLGRFFLWLLVEFISRRMDQLNFPIS
                     SLEVAKGMSSEVVQLLEPASGRIVYTGWLRRLKSSGHVIYAGLYSTVQVPGEPNPCVK
                     VTFPCNGSANVYLSPIAHPDGSFGLDSSGSAWGRSGFYRVVRSGAEHRRVRYVKTLHE
                     LFHVYVDDEDVLRTEHIISFLGLTIIRLHYKMSPASSHTNTIATMRTTVAIEQPR"
     gene            6751..7590
                     /gene="proC"
                     /locus_tag="VGR03_02085"
     CDS             6751..7590
                     /gene="proC"
                     /locus_tag="VGR03_02085"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV1993096.1"
                     /translation="MASNTTQKLSDKKLAVLGTGKLGGILLRAYLKQELFSPKRVTAT
                     VKHAEKAAALSKELGVTATTENRKAVHGADIVLLSVKPQVVGDVLKEIAPELNEKTLV
                     ISVAASVPTSYIEQRLGGKVPVVRAMPNTPSAVGCGMTGICRGAHAGEVHLETARAMF
                     DAVGRTVVVDEKNMDAVTGLSASGPAYAYIILESLAEAGVKVGLPRDVATLLAAQTMK
                     GAASVVLETGDHPALLKDAVTTPAGCTIDGIMELEEGKLRVTLIKAVVKATSRAGELL
                     FDK"
     gene            7640..8014
                     /locus_tag="VGR03_02090"
     CDS             7640..8014
                     /locus_tag="VGR03_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEV1993097.1"
                     /translation="MQSAYIPVLIYAALVMAFPAVTLVLAKFIRPASTSVGAKLKPYE
                     CGIPPESDARGRYSVRFYIVAMLFVIFDVETMFLIPWAILYRGWVAAHQGLFALVSMV
                     LFLGILLVGYLWLYKKGALEWA"
     gene            8257..>8827
                     /locus_tag="VGR03_02095"
     CDS             8257..>8827
                     /locus_tag="VGR03_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEV1993098.1"
                     /translation="MSTPSAKPADTSAPAAPPKAAPAPPKPPVVLQTPLDNEFVTRFR
                     TKFGAALLEATEDRKQAILTVDRGWLAEISLHLRDEEKFDMLADLTAVDWPKREKRFD
                     VVLNLYSFPKNERLRLKVHAADAEEVPSVVSVWPTANWQEREVFDMFGIIFSGHPNLK
                     RILLPDEWQGYPLRKDYDILQQDTAWVREN"
BASE COUNT         1873 a         2334 c         2951 g         1669 t
ORIGIN      
        1 atcgctgggt tccactagca ggctgctgaa aaagtcccat tcctgttatt gcgaggagct
       61 gcaggcgacg gggaatctgc ttttttctta gcttttctgc aaaaagcaga tcccttccga
      121 caaagtcgga atctggcgat cgcctgcgct cgggatgaca acaaaaagag tttttcagca
      181 acctgctaga aatccatcca caccatgctc gacgacttcg gggacgccgc cgacgttggt
      241 ggcgatgacg ggaacttcgc aggccatggc ttcgagggcg gcgaggccaa aggactcgag
      301 ctgcgagggc atcaggaata ggtcggcggc ggcgagtctt tcgtagacgg tgtcctgttt
      361 gccgaggaaa aagacgccct tctgaagttt tttcttgcgg acgaggtatt cggcggcgcc
      421 gcggtccggg ccgtcgccga tgaggacgag gttgccgcca aggtggcgcg ggatgtgcaa
      481 cctctggaag cggtcgtggt gcggtctaac ttcgcagggg aatgagcgcg cggaggcggt
      541 tttcgcggag gtagagtcct gcccagacga gcacgccaaa aatgatgggg aagtagaacg
      601 gttgaccggc gcgcacgtgg gtgacggttg cgccgccaag ataagccgtc agcaagatgg
      661 cgccgaggat cgaggtaggc ggaatcacgt agacgatcgt gcagaggagc aggacggcgc
      721 cgatggggac caccaggctg ttggagaatc ctagctgggt gaatgccgca aggacaggag
      781 cgattttcat caatttagcg atggcatcga agagcaagaa caatacaacg agtgtactga
      841 tgatgcgtcc ggcccagagg cttgtcttcg agacgggttg agtagctgat tccatgaatt
      901 ttcctccaga gatgttaccg attgaaagcc tacggattgt aaactcgttt gcggtgataa
      961 agaaattcag gagcagagcc gccccttagc cgccggcgat ggcgccgatg atgagcaggg
     1021 gctctgctcc cgaagctacc gcgtctggta gcggggtgtc tgcgggctgg agggaatagt
     1081 cttccgcgca ggcgaagaag cgcaggaatg gacggcgctc ttgcgtgaca tgatcgcgga
     1141 tcgtgccgcg cagcatggga tactgtgcct cgagggcgtc gaggacagag cgctgggtaa
     1201 cctgacctac gacctcgagc tgcacctcgg aactgacgcg cgccagcgtc cgcagatgcc
     1261 ccggaagtat gacgcggatc atcgcagcgt ctggacctcc acggaaagca cgccgggaag
     1321 gtggctgacg atgggggtcc aactgtctcc ggcgtcggcc gaggcgtaca cctgcccgcc
     1381 ggtggtgccg aaatagatgc cgcacttatc gagcgtgtcg acggccatcg cgtcgcgcaa
     1441 tacgtttacg tagcagttgt cttgcggcag gcctttggta agcgcttccc attcgtttcc
     1501 tcccgtgcgg ctgcggtaca cgcgcagctt gccatcaggc ggaaagtgct ccccgtcgct
     1561 cttgatgggg acgacgtaga tggtctccgg ctcgtgcgcg tgaacgtcga tcacgaagcc
     1621 gaagtcggtg ggcaagttgc cgctgacctc ttgccaagag tcgccggcgt tgtcgctgcg
     1681 catgacgtcc cagtgctttt gcatgaacag cacgcccggg cgcgacgggt gcatcgcgac
     1741 gtgatgaacg cagtggccga cctcggcagt gggttcggga atgtgttggg aatgtagtcc
     1801 tttattaatt agccgccagg tcttaccggc gtcgtcggtc cggaatgcgc ccgcggcgga
     1861 gatggcgatg aagatccgct ggggatcgct cggatccaga atgatggtgt gcaggcacat
     1921 cccaccggcg cctggcgccc agcggggccc ggagccatgg ccgcgcagtc cggcaagctc
     1981 ttgccagttc tgcccgccgt cggtcgagcg gaaaatggcg gcgtcctcta ctccggcata
     2041 gacagtgtcc ggatcggtag gcgagggttc gagatgccag acgcgcttga actcccaagg
     2101 atgttgggtg ccgtcgtacc actgatgagt ggtgagcggt ttgccggttt cggaggagct
     2161 gtcgtacgcg aacttgttgc tctcgccttt cggcattccc tctggcgtgg tccgttctcc
     2221 cggcggcgtc cctggctgtg tccaagtttt gccgccgtca tcggaacgct gaatgatctg
     2281 cccgaaccag ccgctgcatt gcgacgcgaa cagccgatcc gggtcgacgg gcgagccctt
     2341 gaggtgatac atctcccagc ccgcaaagtg cgggccgctg acatcccagc gttcgcgctt
     2401 tccgtcggaa gtcagaatga atgcgccttt gcgcgtgccc accagtactc gtaccttgct
     2461 catccctcac cccttcctga actttacgca tgtactctaa ggctatcgaa ggacctcgtc
     2521 cagaacggcc gatcgcgcaa cgatcgttgc ctctgcgagc ggcaagtcac tccgaccgca
     2581 gcgcttcgat cacgttgatg cgcgcggcgc gtgccgcagg ccacgccgag gcaatgaccg
     2641 cggcggctag aagcactact accgagccga ccaccggcaa ggcactcggc atacgcacgt
     2701 cctggaagta gctgcctgcc aggcgcgcca gagcgtagcc gcacgccgcg cctgcgacga
     2761 tgcccaccac ggccatcaca gcgccttcgg tgataacacc cgtcaggaga tgccgcggtt
     2821 cggctccaat cgccaggcga acgccgaact cgcgagtgcg cgcgctgacc gaaaacgcca
     2881 gcacccctgc gacgccgacc aaggcaatgg caagagccac agccgcaaag acaccgaaca
     2941 caacggtgtt gagacgatcc ggagtgagaa cttcggcgcg aacgtcttcg agggtagcgg
     3001 cgcgctcaac gggttggtcg accgacatgt cgcgaatgat ccgcgtgatc ggcgtgacga
     3061 gagaataagg atttgcgctg gtgtggatga agaggcggcc gccaaagatg ggaccctctt
     3121 caaaggggct atagacggtg aggctagggc caggaacaac gtgttcgtcg tcaatatcgg
     3181 cagtcacgcc aatgatgcgg tgcggggcgg tgctaatccc gggtatgaat ttcatcacgg
     3241 gatccgtcca atagacatgc ctattgacag catcctgatt ggggaacatt cgttgagcga
     3301 gagtttgact gacgataacg accggatctt tggtctggcc gtcgagttga ttgaaatcgc
     3361 ggccggcgat gatgggaacg ccgagcgcgg caaaaaagcc gggagagatg gtgcgcaact
     3421 gggcgcgagg atcttcttcg ccgggcccgc ggacgtggcc gtccgcagaa aactggaagc
     3481 cggggccaaa gctgccggca tcgcgccagg gaacgacaat gccgaaagcg gtcttgttca
     3541 caccgggtag cgcatcaatg cgccgcatcg attccttgta gaaatcgacg acttgttcag
     3601 gagttttgcc gtgggaaaca gcggggacgt tgatggcaag cacgtggcgg gtgtcgaggc
     3661 cggtctgcgc cgattgtatc gcgagaagcg tttttagcag catgctggcg ccagcgagga
     3721 gcatgaagga ggccgcgatt tgcgtgacgg caaagatgcg gaggcggcga ctcgtgctgc
     3781 cggtgatgcg cactccgccg ctggtgaggc caggtgtttg cgaggcattg gcagttggaa
     3841 gatgcggaac ataagccagg agtacagcgg cgatgacggc gagtaaggcg ccgacccaaa
     3901 gcatgctgga gtctacggcg aggtcgagag cgcggacgga gaagcgagag gcgtagcgag
     3961 caagcacggc gaggagcggt cgtgcactga ttacgccgag agtggcgcca gcgacgcaaa
     4021 ggatgagact ctccgccagc aaagtccggc gcagtgcgcc agcgctcgcg cccatagccg
     4081 cgcggatggc gagctcgccc tcacggcgga cggtgcgcgc gaggataaga ttggcgacgt
     4141 tggagcaagc gatgataaac accaggccgg aggcggcaag aagaactagg agaacggttc
     4201 gagcgccgga agtgatttgc tcacggagga gtttggagtc gatttggaaa tgtgcttggg
     4261 gcgaataggc gtcgagatgg tccttgatca tcgcgccgta gacggtgcga agttcggcgc
     4321 gggcttgatc aagagtggcg ccgggagcga ggcggccgaa gagttcggtc atgcggtgga
     4381 cacgaccgtt ctgcatggtc gcggacagat gatgggggct ggtgacaatg ttggcaatga
     4441 tttcggtatc cgagggatat gggatagagg gttcgaggac gccgatgata gtggcagatc
     4501 tatcgccgaa gccgctgccg aggcgaacgg tcttaccgat gacagaagga tcgttcttga
     4561 aagtggtcga ccagaaacga taggtcagca cgacgacgcc agcggcttta ggaccgtcgt
     4621 cttgcgggcc gatgagacgg ccgaggacgg gatggaggcc caccacctca aaataggagc
     4681 cgtcaacgac gccggcgcga acttcccggg gctcgccgag gccgatcatc gtgaaaccga
     4741 cggtggagaa ctcgccgaag gagctgagtg ttttgacgcc tccgcgcagg tcttgaattt
     4801 ccggcacgga gaatgggact tccccggggc tgataccagg cgcgctttgt tggatgtaaa
     4861 tgaggcggtc ctcgtcgctg ttgacgagcg gccgaagcag cactccgcga accaggctga
     4921 agatggcggc attggcgccg atgcctagcg ccaacgtgag caccacggtg accgcaagac
     4981 ctttggttcg cacgagcgaa cggagcgcaa ctctcagatc gtgaaggaac aactcggatt
     5041 tgaaattcat cttcccctct ctttaagtca cttggagaac gaatgtcagt ctgccgattt
     5101 tacttgtgaa atgcaagtga aaatatgcta ctgtggaaat atgccgaaga agaaggcgag
     5161 tccaaagtgg cggtccgggt gtccgctgaa tgcttcggtg gagatattgg gcgatcgctg
     5221 gtcgttgctg attattcgcg acatgatgct gcggggattc cggagctata aggaattcct
     5281 ggagtcctat gaaggaatcg cgacgaacat tttggcagat cgcctgcaaa ggctgatagc
     5341 tcatggaatc atcaccaccg agcgagaccc attggacggg cgaaaacaga attactcgct
     5401 aacagagaaa ggaatcgatc ttgcaccggt gctcacggaa atggtccttt gggcagccgg
     5461 acacgaagat accgataatc aagcgcttgt ccagctaata aaaaaggaca aggagcaatt
     5521 tctggcggca gcgcggcaac ggtgggccga aaagacaatt cgttcaacgg ctaggtaagt
     5581 ccgagcggac gggcggttgg gccgaagaac gcacagttta ccgaggctgt tcgatggcga
     5641 cggtggtgcg catggtggcg atggtgttgg tgtgggaaga ggccggagac atcttgtagt
     5701 ggaggcggat gatggtgagg ccgaggaagg agatgatgtg ctcggtgcgg agcacatctt
     5761 cgtcgtcgac gtagacgtgg aagagttcgt ggagtgtctt gacgtagcgg acgcggcggt
     5821 gttctgcgcc ggagcgaacg acgcgataga agccggaacg gccccaagcg gagccggaag
     5881 aatccagacc aaacgagccg tcgggatgag cgatgggact gagatagacg ttggcgctgc
     5941 cattgcacgg aaaggtgacc ttgacgcatg gattgggctc gccaggtacc tgcacggtgg
     6001 agtaaaggcc tgcatagatg acgtggccgg aagatttcaa gcggcgcagc cagccggtgt
     6061 aaacgatgcg gccggaggcg ggctcgagga gctggacgac ttcgctgctc atgcctttgg
     6121 ccacttccag agaagaaatg gggaagttga gctggtccat gcggcggctt atgaattcca
     6181 cgagaagcca cagaaaaaag cggccgagga gagagacttc ggtccagacc tcgaggtgat
     6241 acgcagcagc atgttcataa aaatgacgaa tggccggatg aatggcgtcc gggttgaaac
     6301 ctgggccgcg gagggcgttg aaatcgggaa tcaaacctgc gctgggtgga acttggattt
     6361 gaagattttc gtcgcgcgcg atgcgctcat agattctcgt gccgatgcgg ccacgtttgc
     6421 cgagcgggca gcgtagccaa ggggcatcgg cttcgcgaat gcgcttgccg gtgatgcgga
     6481 tccagatctg gatgccgcct tcgagaaaga aggctatcaa atcgtaaagc gcagaacgtt
     6541 tgtcagcctt tgtcggggcc gcggggttcg aagcagcggt agccgcattg ggaagggaag
     6601 gagcggggtc catggctcta tcttctcatc gatagcgaga ggtttcaggg ccgtgaccga
     6661 gttgtgcccg cgggattaac cgttccaggc ggcggcattt gagaatcttg ggttgaccgg
     6721 tgggggaatc gagtcaacaa tgtttgaacc atggctagca ataccacgca aaaactatcg
     6781 gacaaaaaac tagctgtcct ggggacggga aagctcggcg ggattttgct gcgagcttac
     6841 ctgaaacagg agctcttctc gccgaagcgc gttaccgcga cggtgaagca cgcggaaaag
     6901 gcagccgcgc tctcaaagga attgggcgtg acggccacga ccgagaaccg caaggcggtg
     6961 cacggcgcgg acatcgtgct cctcagtgtt aagccccaag ttgtaggcga tgtgttgaaa
     7021 gagattgcgc cggagctcaa tgagaagacg ctggtcattt ccgttgccgc gtcggtgccc
     7081 acgagctaca tcgagcagcg gctcggggga aaagtcccgg tggtgcgcgc gatgcctaat
     7141 acgccttcgg ctgtgggatg cggaatgacg ggcatttgcc gcggcgcaca tgcgggtgag
     7201 gtgcaccttg agacagcgcg cgcgatgttc gacgccgtcg ggcgcacggt ggtcgtagac
     7261 gagaaaaata tggacgcggt gaccgggctt tcggcgagcg gcccggcgta tgcctacatc
     7321 attctcgagt cgctcgcaga agccggcgtc aaggtgggtc tcccgcgcga tgtcgcgaca
     7381 cttctagcag cgcagaccat gaagggcgcg gcgagtgtgg ttctggaaac gggcgatcat
     7441 cccgcgctgc tgaaggacgc cgtgacgacg cccgcgggat gcaccatcga cggaattatg
     7501 gagttggaag aaggcaaact ccgcgtgacg cttatcaagg cggtggtgaa ggccaccagc
     7561 cgcgccggag agctgctctt cgataagtaa gccggcccaa gattgacaac ggtggtagga
     7621 tgctgcgtgt gagggagtga tgcagagcgc gtacattccg gtgttgattt acgcggcgtt
     7681 ggtgatggcg tttcccgcgg taacactggt cctggcaaaa ttcattcggc cggcatccac
     7741 gtcggtcggc gcaaagctca agccctatga gtgcgggatc cctccggaga gcgatgcgcg
     7801 cggacggtat tcggtgcggt tttacatcgt ggccatgctg tttgtgattt tcgacgtgga
     7861 gactatgttc ctcattccct gggcgattct gtaccgaggg tgggtcgcgg cgcaccaggg
     7921 gctatttgct ctggtttcga tggtactgtt cctcgggatc ctgctcgttg ggtatctgtg
     7981 gctatacaaa aagggtgcac ttgagtgggc gtgagccgta attctcctcc tctccctaac
     8041 cccagcgtcc cgttcgcaag ttgttctgat tgactcggtt tgcagcgtgt gttagaaacg
     8101 cgacgtttga attttcgact gctcctttgt tgcagatggt gaaccatagc ggttcactgc
     8161 agcctgtcgc accccgggtc catggctgac gagcccaagg aatcaaaacc gcctcaagtc
     8221 cccacgggcg acaagcccgc aagcagtaca ccccctgtgt ccactccctc tgccaaaccg
     8281 gcggacacga gcgcgcctgc cgctcctccg aaggcggctc ccgcccctcc caaaccgccc
     8341 gtggtgctgc agacgccgct cgacaacgaa ttcgtcacac gatttcgcac caagtttgga
     8401 gcggcgcttc tggaagcgac ggaggaccgc aagcaggcca tcctgaccgt ggatcgcggc
     8461 tggctggcgg aaatttcgct gcacctgcgc gacgaagaaa aattcgacat gctcgcggat
     8521 ttgacggcgg tcgattggcc gaaacgtgag aagcggtttg acgtggttct caatctgtac
     8581 tccttcccga agaacgagcg gctgaggctg aaagtgcatg cagcggacgc ggaagaagtg
     8641 ccgagcgtgg tgagtgtttg gcccacggcg aattggcagg aacgcgaagt gttcgacatg
     8701 tttggaatta ttttttccgg gcacccgaat ttgaaacgca ttctgttgcc ggacgagtgg
     8761 caaggctatc cgctgcggaa agactacgac atcttgcagc aggacaccgc ctgggtacgg
     8821 gaaaatc
//
LOCUS       DASYVO010000063         4008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_129433, whole genome shotgun sequence.
ACCESSION   DASYVO010000063 DASYVO010000000
VERSION     DASYVO010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129433"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="VGR03_02100"
     CDS             complement(<1..1391)
                     /locus_tag="VGR03_02100"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV1993099.1"
                     /translation="MSRRAIKPVCILALMLLLAVSHAFAQQLATLNVTVNDQSGAGVP
                     GANITLKNKDTAASRTATTGEGGLAVVAGLPAGLYDLTAEHAGLSPRTLPVQLAVGQV
                     ATLTVSLTITARAEKVEVTETVQGVDPEKAEVSQVIDTPKIQDLPISGRDFIDFVLLT
                     PSVNVGRSTAVGAQSPFTETVLKLSFAGVRESHTSFFSLDGIDYTTSISGVQRISPSQ
                     DWVQEFRVVPSPYSGDNGRNLGSVVNTVTKSGTNDVHGSVYEFFRNNNLDAKNLLSAP
                     GFNTLRFNQFGATVGGPIRKDKIFFFGGYEGQRRAESPIYSSFILNCTAGVNCFGPGT
                     PSINTVKTLLGLGTENLGSIVTVQDYDKLIVKTNIVLSDKTFINEAYLFNDSRKKNVR
                     GAAPGEGLPSSYRDNPVRDQTLYGSLIHLFSPKLTSETLLQYGRRKFDLIPKGEGFEP
                     ALSIPDLMSSGGFV"
     gene            complement(1521..1694)
                     /locus_tag="VGR03_02105"
     CDS             complement(1521..1694)
                     /locus_tag="VGR03_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993100.1"
                     /translation="MRQYQKLSADGLAGLAVEAFEKTPRDKVEEIAAQQGLSRESLEP
                     AIERLARKPAHAR"
     gene            complement(1719..2957)
                     /locus_tag="VGR03_02110"
     CDS             complement(1719..2957)
                     /locus_tag="VGR03_02110"
                     /inference="COORDINATES: protein motif:HMM:NF037663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="styrene monooxygenase/indole monooxygenase
                     family protein"
                     /protein_id="HEV1993101.1"
                     /translation="MRKIAIIGAGQSGLQLALGLQKHGYSVTVVSNRTPEQVFSGNVT
                     SSQAMFDNSLQAERDLGINFWEKDCPGVQGVAFTVPAPDGSGKKAIFWESRLDHYFQG
                     VDQRVKFSGWMKEFAKRGGDLQIKAAGVQDLESFAKTHDLVLVASGKGEIGRLFETNK
                     EHSPYSTAMRALSLTYVKGMVPRKPFTAVSFNLIPGVGEYFVYPALTVNGVCEIMVFE
                     GVPGGPMDCWSDVKTPEQHLKRSKEICEKFLPWEAERNHKLELTDDRGIMTGRFAPVV
                     RKPLCKLPSGRVALGVGDTVVTNDPLTGQGSNTACKAAKHYMQRIVERGDRPFDAAWM
                     QETFDQYWNNYAQWVTKWTNSLLIPPAPQILELMGAAQGIAPLAKRIAEAFNNPPDVN
                     PWWFDANEAHKVIGAMQAHA"
     gene            3221..3460
                     /locus_tag="VGR03_02115"
     CDS             3221..3460
                     /locus_tag="VGR03_02115"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEV1993102.1"
                     /translation="MKRQARITSKGQVTMPHEIRRALGVFPGDKLLFEKSGAGFRVRP
                     VRTKSRFAKYRGIGNAKIPAGRKAILRWLGDLRGR"
     gene            3470..3892
                     /locus_tag="VGR03_02120"
     CDS             3470..3892
                     /locus_tag="VGR03_02120"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEV1993103.1"
                     /translation="MTTAIDTNVIVALWDKNDALNRAAQDALDAAVGRGNLTIPALVF
                     AELMACPGRSEAFLDSFLKDTAITVDWNLDESVWRTAGRAFQTYAARRRRQRDSGPRR
                     ILADFLIGAYAFENGHRLLTLDDRLYRAAFPGLRIIKV"
BASE COUNT          789 a         1214 c         1212 g          793 t
ORIGIN      
        1 acaaagccgc ccgacgacat caggtccggg atgctgagag ccggctcgaa accctcccct
       61 ttggggatga ggtcaaactt gcggcgcccg tactggagaa gtgtctcgct ggtcagcttg
      121 ggcgaaaaca ggtgaatcaa gctgccatag agggtctggt cgcgcaccgg attgtcccgg
      181 taggacgagg gcaggccctc accgggcgcc gcgccgcgca cattcttctt acgcgaatcg
      241 ttaaagagat aggcctcatt gatgaaggtc ttgtcgctga gcacaatgtt ggtcttgacg
      301 atcaacttgt cgtagtcctg gacggtaaca atggagccca ggttttcagt ccccaggcca
      361 agaagcgtct tgaccgtgtt gatgctggga gtgccgggac cgaagcaatt gacgcctgcg
      421 gtgcagttca ggatgaacga agagtagatg ggcgattccg cgcgccgctg cccttcgtaa
      481 ccaccgaaga aaaatatctt gtctttccgg atggggccac cgacggttgc gccgaactga
      541 ttgaagcgca gggtattgaa acccggagcg gaaagcaggt tcttggcgtc cagattgttg
      601 ttgcggaaga attcgtagac gctgccgtga acgtcgttgg tgcccgactt ggtgaccgta
      661 ttgaccaccg agcccaggtt gcggccgttg tcgccgctgt aggggctggg caccacgcgg
      721 aattcctgca cccagtcctg cgagggactg atgcgctgta cgccggagat gctggtggtg
      781 tagtcgatgc cgtcgagcga gaagaacgac gtgtggctct cccgcacgcc ggcgaagctc
      841 agcttgagca ccgtctcggt gaaaggagac tgcgcgccca ccgccgtgct gcggcccacg
      901 ttcaccgagg gcgtcagcag aacgaagtcg atgaaatcgc gcccggagat gggcaggtcc
      961 tggattttcg gcgtgtcgat gacctggctg acttcggcct tctccgggtc gacgccttga
     1021 acggtctccg tcacttcaac cttttccgcg cgggcagtga tggtgagcga cacggtgagc
     1081 gtggccactt ggccgacggc gagctgcacg ggcagcgtgc gcgggctcag cccggcgtgc
     1141 tcggcggtga gatcgtacag gccggccggc aggcccgcca cgaccgccag gccgccctca
     1201 ccggtcgtcg ccgtgcggct ggctgccgtg tctttgttct tcagcgtgat gttcgctccg
     1261 ggcaccccgg cgccggattg atcgtttacc gtcacgttga gagtggccag ttgttgcgcg
     1321 aaggcatggc taaccgcgag aagaagcatc aatgcaagaa tacagacggg cttgattgcg
     1381 cgtcggctca ttccccatcc ccctttatct caagaccgca ttattcgcga gtctgccact
     1441 tctcaaacca cagagcaaca aaaatgtccc ctcggttccc aaaatgtatc gacgtagaag
     1501 cccactgcac accggatggg tcaacgggcg tgagccggtt ttcttgccag gcgctcaatc
     1561 gcaggctcga gactttcgcg gctgagaccc tgctgcgcgg caatctcttc cactttgtcg
     1621 cggggagttt tttcgaaggc ctccaccgcc aaaccggcca gaccatcggc ggaaagcttt
     1681 tggtactgcc tcacgccggg cagccaagcc cacggcgtct acgcgtgggc ctgcatcgct
     1741 ccgatcacct tgtgggcctc attggcgtca aaccaccacg gattgacgtc gggcggattg
     1801 ttgaaggcct cggcaattct cttggccagc ggggcgattc cctgcgccgc gcccatcaac
     1861 tccaggattt ggggcgcggg gggtatgaga agcgagttgg tccacttggt cacccactgc
     1921 gcatagttgt tccagtactg atcgaaagtc tcctgcatcc aggccgcgtc gaacggacgg
     1981 tctccgcgct ccacaatgcg ctgcatgtag tgcttggcgg ccttgcaggc ggtgttcgac
     2041 ccctggccgg tgagcggatc gttggtcacc acggtatcgc ccacacccag agcgacgcgg
     2101 ccggagggca atttgcacag cggcttgcgc actacgggag caaatctgcc ggtcatgatt
     2161 ccgcggtcgt cggtgagctc cagtttgtga ttgcgctccg cctcccacgg caaaaacttc
     2221 tcacagatct ccttgctgcg cttcaggtgc tgctccgggg tcttgacgtc gctccagcag
     2281 tccatcggtc cgccgggcac gccctcgaag accatgattt cgcagacgcc gttgacggta
     2341 agcgcggggt acacgaagta ctcgccgaca ccggggatca ggttgaacga aaccgcagtg
     2401 aacggcttgc ggggcaccat acctttcacg taggtcagcg agagcgcccg catcgccgtg
     2461 gagtacggtg agtgctcctt gttggtttcg aacaggcgtc cgatttcccc tttgcccgaa
     2521 gccaccagaa caaggtcgtg ggttttcgca aaagactcca ggtcctgcac gccggctgct
     2581 ttgatctgga ggtcgccgcc gcgcttggca aattccttca tccatcccga aaacttcacg
     2641 cgctggtcca ctccctggaa atagtggtcg agccgcgact cccagaaaat cgcctttttg
     2701 cccgagccgt cgggcgccgg aacggtgaag gcaacaccct gcactcccgg acagtccttc
     2761 tcccagaagt tgatacccag atcgcgctcg gcctgcaggg agttgtcgaa catggcctgg
     2821 ctggaggtca cattgccgct gaacacctgc tcgggcgtgc ggttggagac cacagtgacc
     2881 gaatagccgt gcttctggag accgagggca agctgcaggc ccgactgccc ggctccaatg
     2941 atggcgatct tcctcatgat tcctccggaa agcggaaact tttgaacgaa tcgcatccag
     3001 gaacaaggca ttatcaaccg cacagagtcg atgtcaagac gaaacggtta cccacaactt
     3061 gcccgatttt caagaccagg agacgaaccc ggaaacgtat ctcggtgtca ggctgctcat
     3121 ggacaaaccg tgcagaagag tacccccttc tgctcccttg ggaaaatcgg attgtggaag
     3181 taaggagcta cgtgataaag taaggaatag gagattcgct ttgaagagac aagcgcgtat
     3241 caccagcaaa ggccaggtca cgatgcccca tgagattcgc cgagccctgg gcgttttccc
     3301 gggcgacaaa ttgctctttg aaaaatccgg cgcggggttt cgcgttcggc ccgtgcgcac
     3361 gaagagccga ttcgcaaaat accggggcat tggaaatgcc aagatccccg ccggcaggaa
     3421 ggcgattctt cggtggctag gtgacctgcg gggacgatga ccacagccga tgaccacagc
     3481 catcgatacc aacgtcatcg tcgccctgtg ggacaagaac gacgcgctca accgggcggc
     3541 acaggacgct ctcgatgcgg ccgtcgggcg cggcaacttg acgattcctg cgctggtctt
     3601 cgcggagctg atggcgtgtc ccggccgaag tgaagccttt ctggattcct ttctcaaaga
     3661 cactgcgatc acagtcgact ggaatctgga cgaatccgtc tggcgaaccg ccggccgggc
     3721 cttccaaacc tacgccgccc gccggcggag gcagcgcgat tccggaccgc gccgcatttt
     3781 ggcagacttc ttgatcgggg cctatgcctt cgaaaacggt caccgcctgc tcacccttga
     3841 cgatcgcctc tatcgcgcag ccttccccgg ccttcgaatt atcaaggtct aattgcaaag
     3901 cttcggcgcg gcacagatgt ggcaccccca acgtgtgcca tggcaagccc ccgcagccct
     3961 ggacatcggc gggacgaatt tcgccgttgt tggccgtaag ttcacatg
//
LOCUS       DASYVO010000064         4392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_226657, whole genome shotgun sequence.
ACCESSION   DASYVO010000064 DASYVO010000000
VERSION     DASYVO010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226657"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VGR03_02125"
     CDS             <1..703
                     /locus_tag="VGR03_02125"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1993104.1"
                     /translation="FAEAAKTFAPLGVQGMQDSAVGYAWAASLARTGELKQASEVLSQ
                     YEKGNRSPESLLLVGQLWIEIGDYARSVASLHRALQADSGLPKAHYFSGQAYIRWEHW
                     SEAADEFKAELVLVPTDPDAKFNLGFVSLQQSNVEEAAELFKQVITLHPDHAKSQYEL
                     GKILMDRGQMKEAIDHLEAAARLSPQTDYIHYQLQAAYRKESRIADADRELELYKELK
                     AKSRERTVPQPVQRP"
     gene            765..2543
                     /locus_tag="VGR03_02130"
     CDS             765..2543
                     /locus_tag="VGR03_02130"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEV1993105.1"
                     /translation="MVSLLAARLAHGQGGHPQAPPPPPGAKSAKCSGRPIPQLQDVTA
                     KAGITFKHTADPAKKYIVESMSGGVILLDYDRDGWLDIYFTNAPTVEMAIKGQKSRGA
                     LYHNNHDGTFTDVTEKSGLTSDCFAMGGAIGDYDNDGWPDLYLTCLGGNVLYHNNGDG
                     TFTDVTAKAGVADGRWSTGAAFGDYDGDGFLDLMVTNYVDFHLNDLPGFGKSPNCKYR
                     GIDVQCGPRGLKGAGDALFHNNGDGTFTDVAKSAGVDDPNGYYGLGVVWADFNNTGRP
                     DIYIANDSTPKFLYKNLGNGKFADIGLESGTAVSEDGSEQASMGIAIGDYLHTGRPSL
                     YVTNFSDENDLLYRNDGNWNFTDVSYASGVALPSLPFVKWGTAFADLDNDGWLDLITV
                     TGHVYPQVDSLPSGAGYREPKLLRMNQKDGTFCDASGQAGPALLEKRVSRGVAVGDLF
                     NTGNLDVVIGDIDGAPMVLKNQGVAGRHWVSFELAGTKSNRLAIGARVKVIAGGMTQT
                     DEVHSGGSYLSQNDLRLHFGLSTAAKIDRVEIRWPSGKLDTRTDLPADQFYSVLEGTG
                     VMPAERIRPVPLVKAKPATAPGQASP"
     gene            2728..>4392
                     /locus_tag="VGR03_02135"
     CDS             2728..>4392
                     /locus_tag="VGR03_02135"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HEV1993106.1"
                     /translation="MNTRRALQSGIVFFLLIAVSAARAGDDIPKAAWKRPIGAPLEHS
                     GTKKPALDSGHIDDGYWQGAPVGGFGSGTFSRTYRGDFARWHMQGGVHKYQIVDGNQF
                     AMFQKSEDGGEGVAQVLTANHPKDHSLSSWKWDYPVGAGDYAALYPKSWFDYRWDKFP
                     AHVVLEQFSPVLPDNYKESSYPVAVYRWHAENPTKKRVTVSVLLSWENMLGWFRTFNR
                     DLSGGINAGNFNRHTSENLGATGTMKGVIFDRNHIGPVQDAWDGQWTIAALESPGVEV
                     TYQTTFAAGGDGKEVWEGFSKDGRLSNSDNSWTSAGESLAGAIAVRFTLEPGEKRVIP
                     MVIAWDQPIVQFGAGRKWSRRYTDFYGTSGMNSWAIARDGLKNASAWSEAINAWQAPY
                     VNDESKPLWYRGMLFNEIYVLADGGSFWGRPVGSDPKLPPVYSFMECYDYPFFETLDV
                     RFYGSMPLIKFWPDIEKGVMREFADTVPHEYNEKMIWIWKSLQTGSATFKIRKAKGAV
                     PHDLGVPEEDPFMQINQFSWQDTNGWKDLNPKFVLMVYRDFVLTGGN"
BASE COUNT          926 a         1313 c         1234 g          919 t
ORIGIN      
        1 gttcgcggag gcagcgaaaa ccttcgcccc gcttggcgtt cagggcatgc aggattctgc
       61 cgtgggctat gcttgggccg cttcgctcgc ccgcaccggc gaattgaagc aagcctccga
      121 agttctgtcg cagtatgaaa agggcaatcg ctctccggag tcgctcctcc tggtcggtca
      181 gctttggatc gaaatcggcg attacgcgcg ctccgttgcc tcgctgcatc gcgccttgca
      241 agccgactct gggctgccga aggcccacta cttctctggc caagcctata ttcgttggga
      301 gcactggtcc gaggcggccg acgaattcaa agcggagctc gtcctcgtac ccaccgaccc
      361 agatgccaag tttaaccttg gctttgtcag cctccagcag tccaacgtcg aggaggctgc
      421 ggaactcttc aagcaggtca ttactctcca tcccgatcac gccaagtcac agtacgaact
      481 cgggaagatc ctaatggacc gcggccagat gaaggaggcc atcgatcacc tggaggccgc
      541 ggcgcgcctc agcccccaga cggactacat ccactatcaa ttgcaggccg cttaccggaa
      601 agagtctcgc atcgcggacg ccgaccgcga gctcgaactc tacaaggagc tcaaggccaa
      661 gtcgcgcgaa cgcaccgtgc cgcagccggt tcaacgccca tgatattctt tccatgggaa
      721 accctttgat cggacattgc tggagaagac ccgcgaaggt tttgatggtt tcgcttttgg
      781 cggctcgcct tgcacatggg cagggtgggc accctcaagc gcctccgccg ccaccgggag
      841 cgaagtccgc caagtgcagc ggccggccga ttcctcaact ccaggacgtc accgcgaagg
      901 cgggaatcac gttcaagcac actgccgatc ccgcgaaaaa atatattgtc gagtccatga
      961 gcggcggcgt gatcctcttg gattacgatc gcgacggatg gctggacatc tatttcacga
     1021 atgcgccaac cgtggaaatg gccatcaagg gccagaagag ccgcggcgct ctttatcaca
     1081 acaatcacga tggaacgttt accgatgtca cggaaaaatc cggcttgacc tcagactgct
     1141 ttgccatggg tggagcgatc ggtgactacg acaacgacgg ctggccggat ctttacttga
     1201 cctgcctcgg cggcaacgtt ctttaccaca acaacggcga cggcactttc accgacgtga
     1261 ccgccaaggc cggcgttgcc gatgggcgct ggtccaccgg cgccgctttc ggcgattacg
     1321 acggcgacgg gttcctcgat ctgatggtca cgaactatgt cgattttcac ctcaatgacc
     1381 ttcccggctt cggcaaatct cccaactgca aatatcgcgg aatcgatgtg cagtgcggcc
     1441 cccgcggatt gaagggcgcg ggcgatgcgc tcttccacaa taatggcgac ggcacattca
     1501 cggacgttgc gaagagcgcc ggtgtcgatg atccgaatgg ctactacggt ctcggtgttg
     1561 tctgggcgga tttcaataac accgggcggc cggatattta tatcgccaac gattcgaccc
     1621 caaaattttt gtacaagaac ttaggcaatg gaaaattcgc cgacatcgga ctcgaatccg
     1681 gaacggccgt cagcgaagat ggctccgagc aagcttccat gggcatcgcc atcggcgatt
     1741 acctccacac gggccggcca tcgctctatg tcacaaactt ttctgacgag aacgatctcc
     1801 tttaccgcaa cgacggcaac tggaatttca ccgatgtctc ttacgcgtca ggtgtggctc
     1861 tgccgtcctt gccgttcgtc aagtggggca ccgccttcgc cgacttggat aacgatgggt
     1921 ggctggatct gattaccgtg acgggccacg tttacccgca ggtggattcg ctgccttcgg
     1981 gcgccggtta tcgagagccg aagttgctgc gcatgaatca aaaagacgga acgttttgcg
     2041 atgccagcgg tcaggcaggg ccggcgctcc tcgagaagcg cgtttcccgc ggggtcgctg
     2101 tcggcgatct tttcaatacc gggaatttgg acgtcgtgat cggcgatatc gacggcgctc
     2161 ccatggtctt gaaaaatcag ggtgtcgctg gccgacactg ggtcagtttc gagttggcag
     2221 gcacgaagag caaccgcctg gcgatcggcg caagagtcaa agtgatcgcc ggcggaatga
     2281 cgcaaaccga cgaggtccac agcggcggca gttacttgtc gcagaacgat cttcgtttgc
     2341 actttggttt gagtaccgcg gccaaaatcg atcgcgtcga aattcgctgg ccctccggca
     2401 aactggacac gcgcacggat ttgcctgccg accagtttta ttccgttctg gaggggaccg
     2461 gcgtaatgcc tgcggaacgc attcgcccag ttccgctcgt caaggccaaa ccagcgacag
     2521 cgccggggca ggcgtctcca taacgtctgt gcgtatccac agaccagcag gattccccga
     2581 aacgcgctcc tcgcgggcat gaactcgcag gtgccaagca tcaactattt gattgctaag
     2641 aataaccata ttctatagga tgtaagcgct tacggtcgct tgctttttcg ctacgcagag
     2701 acctgagcgc gattcctcgg aggcttcatg aacacccgca gggctcttca atctggcatt
     2761 gttttcttcc tgctgattgc cgtttcagca gctcgcgctg gcgacgatat cccgaaagcc
     2821 gcctggaagc gccccatcgg cgcaccgctc gaacattcgg gcacaaaaaa accggcactg
     2881 gactccggcc acatcgatga tggttactgg cagggcgcac ccgtcggcgg tttcggttcc
     2941 ggcacgttct cgcggaccta tcgtggcgat ttcgcccgct ggcacatgca aggcggcgtt
     3001 cataagtatc agatcgtgga cggaaatcag ttcgccatgt ttcaaaagtc ggaggacggc
     3061 ggcgaaggtg tcgcgcaggt cctcaccgcg aaccatccaa aggatcactc gctcagctcc
     3121 tggaagtggg attaccccgt cggcgcggga gactacgctg cgctgtatcc aaagtcctgg
     3181 ttcgattacc gctgggacaa atttcccgct cacgtcgtgc tcgagcagtt ttctcccgtt
     3241 cttcccgaca attataagga atccagttat cccgtcgccg tctatcgctg gcatgcggag
     3301 aatcccacga agaagcgcgt cacggtttcc gttttgctct cctgggaaaa catgctcggc
     3361 tggtttcgca ctttcaaccg cgacctcagt ggcggcatca acgccggcaa tttcaatcgc
     3421 cataccagcg agaatcttgg cgctacagga acgatgaagg gcgtcatctt cgaccgcaat
     3481 cacattggcc ccgtgcaaga tgcctgggac ggccagtgga ccattgcggc gctggaatcg
     3541 cccggcgtcg aagttaccta ccagaccaca tttgctgccg gcggcgacgg aaaagaagtc
     3601 tgggagggat tcagcaaaga cggccggctt tccaatagcg acaattcttg gactagcgct
     3661 ggagagtctc tggccggcgc catcgctgtg cgtttcacgc tcgagcctgg tgaaaaacgt
     3721 gtcatcccga tggtcatcgc ctgggatcag cccatcgtcc agtttggcgc cggccgcaaa
     3781 tggagccgcc gttacacgga tttctacggc acatccggaa tgaattcctg ggccattgcg
     3841 cgcgatggtt tgaagaatgc ctcggcctgg agcgaagcca tcaatgcgtg gcaggcccct
     3901 tacgtcaacg acgagtccaa accgctttgg tatcgaggca tgctcttcaa cgaaatctac
     3961 gtcttggccg atggtggttc cttctggggc cgccccgtgg gatccgatcc gaagctgcct
     4021 cctgtttatt cgttcatgga atgctacgac taccctttct tcgaaacgct ggacgtgcgc
     4081 ttctacggct ccatgccact gatcaaattc tggccggaca ttgaaaaagg cgtgatgcgg
     4141 gaatttgccg acaccgttcc gcacgaatac aacgagaaaa tgatctggat ctggaaatcc
     4201 ctgcagaccg ggtccgccac tttcaagatt cgcaaagcca aaggcgctgt tccccacgat
     4261 ctcggcgtgc ccgaagaaga tcccttcatg cagatcaatc aattcagttg gcaggacacc
     4321 aacggctgga aggatctcaa tcccaagttc gtgctgatgg tctaccgcga ctttgttctc
     4381 accggcggca ac
//
LOCUS       DASYVO010000065         3839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_44567, whole genome shotgun sequence.
ACCESSION   DASYVO010000065 DASYVO010000000
VERSION     DASYVO010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44567"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VGR03_02140"
     CDS             <1..390
                     /locus_tag="VGR03_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEV1993107.1"
                     /translation="QFMTDADGLHGSFLTTDENGQRLFAITSSDGTSQNAALTVVQLA
                     AVPLGIGTITPSSVPAVGGAALTIRGSGFQSAATLTINGKAASVTFKDAGTLLVVTPS
                     LTPGSQQITITNPDGEAISLDAAFIAN"
     gene            530..1501
                     /locus_tag="VGR03_02145"
     CDS             530..1501
                     /locus_tag="VGR03_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV1993108.1"
                     /translation="METRKLGNSDIKITPVGFGAWALGGGGWEFAWGPQDDADSIAAI
                     HRALELGVNWIDTAAVYGLGHSEEVVARALKEWRGPRPHVFTKCVLCWNDQGKIWKQF
                     TPDSLRRECEASLRRLQIESIDLYQMHWPPDDGKPGLEEAWQTLAALRAEGKVRWIGV
                     SNFNAAQITRAEKIALVTSLQPPYSLIRRQIEAETLPYCEKRGIGVISYAPMASGLLT
                     GAMTRERAAALPADDFRSRNPEFHEPRLSKNIELVERLRQVGARHRRTPGEVAIAWTL
                     RRPMVTAAIVGARNAKQSEGVMRAGELKLSAEEIAEIEGATAAATAR"
     gene            complement(1583..2017)
                     /locus_tag="VGR03_02150"
     CDS             complement(1583..2017)
                     /locus_tag="VGR03_02150"
                     /inference="COORDINATES: protein motif:HMM:NF040124.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6526 family protein"
                     /protein_id="HEV1993109.1"
                     /translation="MTEQNLGNHAKFVPPFHFFAVPVLVINFGWSLYRLRSLGFSFAG
                     VFGVLLAAALVIVVFSARLFALAVQDRVIRLEERLRYAQVLPSDLQARTGELAIGQIV
                     SLRFASDAELPALARKVLDEKLTERKAIKQLIKSWKPDYQRA"
     gene            2240..2524
                     /locus_tag="VGR03_02155"
     CDS             2240..2524
                     /locus_tag="VGR03_02155"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEV1993110.1"
                     /translation="MAKASWGGKVIAESNATVVVEGNQYFPPDAVKKELLKPSNHTTV
                     CPWKGTAHYYHVEVDGLRNDNAAWYYPEPKPAAAEIKDRIAFWNGVRVEA"
     gene            complement(2585..>3839)
                     /locus_tag="VGR03_02160"
     CDS             complement(2585..>3839)
                     /locus_tag="VGR03_02160"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV1993111.1"
                     /translation="SGGQGAAALTVIRAGTLIDGVSEAPRKNQLIFVRGERIEKVADG
                     STAIPAEAKIIDLSNATVLPGLIDAHTHIFLWGEDPAKGGYDANILKAGIALRAARAT
                     VAARRALEQGFTTLRDVETEGAGYGDIEIKQAIEEGTIPGPRIFGATRAISTTGGYNL
                     EGYAPELDMPKGAQLVDGPVEARKAARQQLEHGADWIKVYMTHRSWVDKEGKLVSQPT
                     LTVEELKAIVDETHGWQKKVACHAYNGIGLQRALDGGCDSIEHGLEITDAQIAQMKRQ
                     GTWYCPTLSPYYGDWAPAETPEGKRDRARAAVHEISFKKALQAHLKIVYGTDMGGIPW
                     TEPMAQEFKRMVSLGMAPMDAIHSATSRAAEMLDMKGEIGVIAAGAYAEVIAVSGDPL
                     KDVGELEHVWFVMHDGAIFKNEMGK"
BASE COUNT          769 a         1319 c          951 g          800 t
ORIGIN      
        1 cagttcatga ccgatgccga cggccttcac ggcagctttc ttactaccga cgaaaatggc
       61 cagcgtctct ttgccatcac ttcctcggat ggcacatccc agaacgccgc cttaaccgta
      121 gttcaactcg ccgccgtccc cttgggaatc ggcaccatta cgccttccag tgttcccgca
      181 gtcggtggcg cggctctcac catccggggc agcggctttc agagtgccgc cactctcacc
      241 atcaacggaa aagctgccag tgttacattc aaggatgccg gcactcttct cgtcgtaact
      301 cccagcctca cccccggatc tcagcagatc accatcacca atcccgacgg cgaagcgatt
      361 tcgctggatg ctgccttcat cgccaactaa gcgagtgctg gcctcttttc tctcctctgg
      421 acgccttcta accttttttg cggtatgttc atccaacgtt gggagaataa aaaaacagat
      481 tcgctacgcg ggaagttcgc ccagcgcgcg actcgagaag aggctccgaa tggaaacgcg
      541 aaaattggga aactcggata tcaagataac gcccgtcggc tttggcgctt gggccctcgg
      601 tggtggcggc tgggaattcg cctggggccc gcaggatgat gccgattcca tcgccgcgat
      661 tcatcgcgca ctggaactgg gcgtcaactg gattgacact gccgccgttt acggtctggg
      721 ccactccgaa gaagtcgttg cgcgcgcgct gaaggaatgg cgcggcccgc gaccccatgt
      781 tttcaccaaa tgcgttttgt gctggaacga ccagggaaaa atttggaagc aattcactcc
      841 cgattccctt cgccgcgaat gcgaagccag tctccgccgc ctgcaaatcg aatccatcga
      901 tctctaccaa atgcactggc ccccggacga cggcaaaccc ggcctcgaag aagcctggca
      961 aacactcgcc gcgctgagag ccgaaggcaa ggtgcgctgg atcggcgtgt ccaatttcaa
     1021 cgcagcccaa atcacgcgcg ccgaaaagat tgccctggtc acctctttgc agccgcccta
     1081 ctccctcatt cgccgccaaa ttgaagccga gactcttcct tattgcgaaa agcggggaat
     1141 cggtgtgatc tcttacgcac ccatggcctc cggtctgctc accggagcca tgactcgcga
     1201 acgcgcggca gcgttgccgg ccgatgattt tcgcagccgc aatccggaat ttcacgaacc
     1261 acgcttgtcg aaaaatatcg agcttgtcga gcgcttgcgc caggtgggcg cccgccaccg
     1321 ccgcacgccc ggtgaagttg ccatcgcgtg gaccctccgc cgacccatgg taaccgcagc
     1381 gatcgtgggc gcccgcaacg ccaaacagtc cgaaggcgtc atgcgtgccg gcgaactgaa
     1441 gctcagcgcg gaagaaatcg cagaaatcga aggcgcaacc gccgcggcca ctgctcgctg
     1501 aatttaatca agcagcggcg gagttaaaat gcagcgatca gacgctgcag tccttaaagc
     1561 aatttttcgg aaccaagaag agctacgccc gctggtagtc cggcttccaa ctcttgatga
     1621 gttgcttgat cgctttgcgc tccgtcagct tctcatccag cacttttctc gcgagcgctg
     1681 gcaactcagc gtcacttgcg aaccgcagcg acacgatttg tccgatggcg agctcgccgg
     1741 ttcttgcctg caaatccgac ggaagcactt gcgcgtatcg caaccgctct tccagccgga
     1801 tgacccgatc ctgcaccgcc agcgcaaata gccgcgcgct gaagaccaca atcacgagtg
     1861 ccgcggcgag cagtacgcca aagactcccg cgaacgaaaa tcccaaactc ctcaatcggt
     1921 acaaggacca cccaaagttg atcaccaata ccggtaccgc aaaaaaatgg aacggtggta
     1981 caaactttgc atggtttccc aaattctgct cggtcattgc tcttcctcca ttgtggcgct
     2041 cgattgtagc aaacccccgc cgcctttccc gcaggactca cccggcgccg attcctcagt
     2101 ttcgatgcgc ggcctttttt tcgttgcgcg cggatttctg caccaatccc gccgcaggcg
     2161 catccaatcc cttggacaaa ttcatttgtc cggcgatatc ctgatcggga gcaatccttc
     2221 tgcgccggag gcgcaccaaa tggccaaagc atcctggggc ggaaaagtga tcgcggagag
     2281 caacgccact gtcgttgtcg aaggcaatca gtatttcccg cccgacgctg taaaaaagga
     2341 actcctgaag cccagcaatc acactacggt ctgtccgtgg aagggcaccg cccactacta
     2401 ccatgtcgaa gtggatggcc ttcggaacga caatgccgcc tggtattacc ccgaacccaa
     2461 gcccgcagcc gctgaaatca aggaccgcat cgccttctgg aatggcgtcc gcgtcgaagc
     2521 ctagttcccg ccggaatcta gctcgcccgg ctttgtaggg cccgccttct gaggcgggcc
     2581 gttcctactt ccccatctca ttcttgaaaa tcgctccatc gtgcatcaca aaccacacgt
     2641 gctccaattc tccaacgtcc ttcagcggat ctcctgacac cgcgatcact tccgcgtacg
     2701 caccggcagc gatcaccccg atctctccct tcatgtccag catctccgcc gctcgcgatg
     2761 ttgccgaatg aatcgcgtcc atcggcgcca ttcccaaaga gaccattctc ttaaactctt
     2821 gcgccatcgg ttccgtccac ggaattccgc ccatatccgt cccatacacg attttcaaat
     2881 gcgcttgcag tgccttcttg aaactgattt catgcaccgc tgcccgcgcg cggtcccgct
     2941 ttccctccgg agtctccgcc ggcgcccaat cgccgtagta cggcgaaagc gtcgggcaat
     3001 accacgtccc ctgccgcttc atctgcgcga tctgcgcatc ggtaatctcg agtccatgct
     3061 cgatcgaatc gcatccgcca tccagcgcgc gctgcaatcc gattccgttg tacgcatggc
     3121 atgccacttt cttctgccat ccgtgcgttt catccacgat ggctttcaac tcttccaccg
     3181 tcagcgtcgg ttgcgacacc agctttccct ctttgtcgac ccacgaccgg tgcgtcatgt
     3241 acaccttgat ccagtcggcg ccgtgctcca gttgctgccg cgcggccttg cgcgcttcca
     3301 ccggtccatc gacaagttgc gcgcccttgg gcatatccag ttccggcgcg tacccttcca
     3361 ggttgtatcc gcccgtcgtc gagatcgccc gcgtcgctcc aaaaattctc ggcccgggaa
     3421 tcgtcccctc ttcgatcgcc tgcttgatct cgatgtcgcc gtaccccgcg ccttccgtct
     3481 ccacgtcccg cagcgttgta aaaccctgct caagcgcacg ccgggccgcg acagtggcac
     3541 gcgccgcccg cagcgcgatt cccgctttca aaatattcgc gtcgtaaccg cccttcgccg
     3601 gatcctcgcc ccataagaaa atatgtgtgt gcgcatcaat cagccccggc agcactgtcg
     3661 cgttcgagag atcgatgatc ttcgcctccg ctggaatcgc cgttgaaccg tccgccactt
     3721 tttcaatccg ctccccgcgc acaaaaataa gttggttctt ccgcggcgct tcgctcaccc
     3781 cgtcgattaa cgtccctgcg cgaatcaccg tcaacgccgc cgcgccctgt cccccgctt
//
LOCUS       DASYVO010000066         3875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_114078, whole genome shotgun sequence.
ACCESSION   DASYVO010000066 DASYVO010000000
VERSION     DASYVO010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114078"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1660)
                     /locus_tag="VGR03_02165"
     CDS             complement(<1..1660)
                     /locus_tag="VGR03_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV1993112.1"
                     /translation="MAIAPVVNRRDFLKTGTAGGVALVIGFHLTPRAFADQAQDQEKK
                     TPNPLDAWVRVTADNNVTLILGKSEMGQGIMTALPMILAEELCLDWKHVKVEQAPTNP
                     KIYDHGTGGSGSIAGSWLPLRRAGAAAREMFISAAAARWKVSRDTCKAQEGGILHGAR
                     KNFLTYGELVSEAAKLPVPDFNTVPLKNSNDFTIVGHDTRRFEGRDKATGAAIFGLDS
                     RVPGMLFAVIARCPVFGGKVKSFDAAKTKAVPGVRDVFAIDPVGQGAFTAGGVAVLAD
                     NSWAAMQGRKALQITWDEGPAASESSASLRQQFLDNAAKPGKVVRNDGDAGAVFAGAA
                     KKTEAIYELPFAPHATMEPMNCTVHMRPDGAEAWVPTQAPQWAQEVIAGVAKLPPEKV
                     IVHTTLMGGGFGRRYQADFVMEAAQVAKVSGKPVMVFWTREDDMQHGFYRPASYHHFS
                     GALDAKGNLTAWKHFQTSTSIEAMWNPKGEENAEKSEFATAAFIPYQTPNYRVEYTLA
                     KSSVPRAWWRSVEHSSSGFVVESFVDELAAAAGADPLEFRLRLIG"
     gene            complement(1705..2184)
                     /locus_tag="VGR03_02170"
     CDS             complement(1705..2184)
                     /locus_tag="VGR03_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV1993113.1"
                     /translation="MAISFTLNGKAQSVDVSPDMPLLWVLRDTLNMTGTKFGCGMALC
                     GACTVHINGEATRSCTTAISSVAGKKVTTIEGLSADGSHPVQQAWMEEDVPQCGYCQS
                     GQIMSAVALLSKKPNPTDIEIDDAMTGNICRCGTYQRIRKAIHRAASMQVGKAKSAA"
     gene            complement(2365..>3875)
                     /locus_tag="VGR03_02175"
     CDS             complement(2365..>3875)
                     /locus_tag="VGR03_02175"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional polysaccharide
                     deacetylase/glycosyltransferase family 2 protein"
                     /protein_id="HEV1993114.1"
                     /translation="GKGNIILLHDGGGDRSQTLAALPQIIDALQAQGYKFVSVSDLIG
                     KTRAEVMVPLSTEERLEARVDGIIFSLYQWLRFLIGTIFILGIVLVSGRAVIIGLLAL
                     IEKLRPDHAVMPDPPPSVTVLIPAHNEESVIVQTVTSVLLSDLQDLRIVVVDDGSTDK
                     TGELLDANFSHEPRVHIVHQVNRGKAAALNLAMSLADTEIVVTIDADTEIESDAISKL
                     LRHFSDPKVGAVAGNVKVGNRSRWLTRWQALEYITSQNMEKRAFDLLNCITVVPGALG
                     AWRKKAIEAAGGITADTVAEDADLTIAIRRLGWRVSYDEEAIAWTEAPETAGQLIRQR
                     FRWTFGTLQSCWKHGDTLLRPKYGTLGWIALPNILVFQLVLPLISPIIDLMFFGSLLL
                     WGLAQFRVTRLPQLWTTSDVEKSVLFFLGFLLIDIVTCMVAFALEDNEDWTLLIPVLL
                     QRFYYRQLMYVVLFRSVKEAVSGRPVGWRGVELEAPAQTSKAPPKPAPAEGN"
BASE COUNT          783 a         1147 c         1180 g          765 t
ORIGIN      
        1 ctccaataag gcgcaggcga aattccagcg ggtcggcgcc ggcagccgcc gcaagttcgt
       61 cgacaaacga ttcgaccacg aagccgctgc tggaatgctc gacggagcgc caccaggcgc
      121 gtggcacgga cgactttgcg agcgtgtatt cgacgcgata gttcggcgtt tgatagggaa
      181 tgaatgcggc agtggcaaat tcggactttt ccgcgttctc ttctcccttg ggattccaca
      241 tcgcttcgat ggaagtggaa gtctgaaaat gtttccaagc ggtgagattg cccttcgcgt
      301 caagcgcacc cgaaaaatgg tgataagaag cgggacgata gaagccatgc tgcatatcgt
      361 cttcgcgggt ccaaaatacc atgaccggct tgccggaaac ttttgccact tgcgcggctt
      421 ccatgacgaa gtctgcctga tagcgcctgc caaagccgcc gcccatcagg gtggtgtgca
      481 cgatgacttt ttccggcggc agttttgcaa ctccggcgat tacctcctgc gcccattgcg
      541 gcgcctgcgt cggcacccac gcttccgcac catcaggccg catgtgcacc gtacagttca
      601 tgggctccat ggtggcgtga ggcgcaaaag gaagttcgta gatggcttcc gtttttttag
      661 cggcaccggc aaaaaccgct ccggcgtcgc catcgttgcg gaccaccttg cccggcttgg
      721 ctgcgttgtc gaggaactgc tggcgaagcg acgcggagga ttccgatgcg gccgggcctt
      781 catcccaggt gatctggaga gctttgcggc cctgcatggc cgcccaggaa ttatccgcga
      841 gaacagcgac accgcccgcg gtgaacgcac cctggcccac gggatcgatg gcaaagacgt
      901 cccggacgcc cggcacggct ttggtttttg cggcgtcgaa ggatttcact ttcccgccga
      961 aaacagggca acgggcgatg acagcgaaaa gcatgccggg cacgcgcgaa tcgagaccga
     1021 agatggccgc cccagtagcc ttgtcgcggc cttcaaagcg ccgcgtgtca tggccgacga
     1081 tggtgaaatc gttggaattc ttgagcggga cagtgttgaa gtccggcacg gggagtttgg
     1141 cggcctccga gacaagttca ccgtaggtga ggaaattctt gcgcgcgccg tggaggatgc
     1201 cgccctcctg cgctttgcaa gtgtcgcgac tgaccttcca gcgtgccgcc gcggcagaaa
     1261 tgaacatctc gcgtgccgcc gcgcccgccc gccgcaaagg cagccaggag ccagcgatgc
     1321 tgccgctgcc gccggtgccg tgatcataaa ttttcggatt ggtgggcgct tgctcgacct
     1381 tcacatgctt ccaatccagg cagagctctt ccgctaggat catgggcagt gccgtcatga
     1441 tgccttggcc catctcggac ttaccaagaa tcagcgtgac gttattgtcc gcagtgacgc
     1501 ggacccacgc gtccagggga ttgggagtct tcttttcctg gtcctgtgcc tgatcggcaa
     1561 aagcacgcgg agtgagatgg aagccgatga ccagggcgac gccgcccgcg gtaccggtct
     1621 tcagaaaatc acggcggttt acaacgggag ctattgccat gggacacctc gggatgtgtt
     1681 gcagtcggtg cgattgtgag tcaattaagc agcggactta gccttgccca cctgcatgga
     1741 cgcggcgcgg tggatggcct tgcggatgcg ctgatacgtg ccgcagcggc agatgttgcc
     1801 ggtcatggca tcgtcgattt cgatgtcggt ggggtttggc ttcttcgaga gcagcgctac
     1861 cgcggacatg atctgtccgg actggcaata gccgcattgc ggaacgtctt cttccatcca
     1921 ggcctgctgg accggatggc tgccgtcggc agagagacct tcgatggtgg tgactttctt
     1981 gccggcgacg ctggagattg ccgtggtgca cgaacgcgtg gcttcgccat tgatgtgcac
     2041 cgtacaagct ccgcagagcg ccatcccgca gccgaacttc gtgccggtca tgttcagtgt
     2101 gtcgcgcaag acccagagca gcggcatgtc cgggctgacg tcgacggatt gggctttgcc
     2161 gttcaaggtg aaactgatgg ccatggagtc ctccctcttg cagatggata tcctacaaca
     2221 actacctgca tagatgaaaa tccgcggaat ctcgttcagg gtgacacaat agggctcttc
     2281 agaaaggaac ggcacctgac actcgaagac atgagcactg cctccacgga atcagacgct
     2341 cggcatcccg aggcgtgagc aagcttagtt tccctcggcg ggggcgggct tgggtggagc
     2401 tttggaagtt tgcgccgggg cttccaactc gacgccgcgc cagcccacgg ggcgcccgct
     2461 caccgcttcc ttcacggatc gaaacagcac cacgtacatc aactgacggt agtaaaagcg
     2521 ctgcaacaaa acgggaatca gcagcgtcca gtcttcgttg tcttccaagg cgaaggccac
     2581 catgcacgtc acgatgtcga ttagcagaaa gcccaggaaa aagagaaccg atttctccac
     2641 gtccgaggta gtccacaatt gaggcagccg cgtgacgcgg aactgcgcca ggccccagag
     2701 cagcagcgaa ccaaagaaca tgaggtcgat gatcggcgaa atcagcggca gcacgagctg
     2761 aaataccaag atattcggca gcgcgatcca gccgagcgtg ccgtatttgg gccggagaag
     2821 cgtgtcgcca tgcttccagc aggattgcaa tgtgccgaag gtccagcgga aacgctggcg
     2881 gatcagttgc ccggctgttt ccggagcttc tgtccaggcg atggcctctt cgtcgtagct
     2941 gacccgccaa ccgagacggc ggatagcgat ggtcagatcg gcgtcttcgg cgacggtgtc
     3001 cgcagtgatg ccacccgcgg cttcaatggc cttcttgcgc catgcaccga gcgctcccgg
     3061 cacaacggta atgcaattca agaggtcaaa cgcacgcttc tccatattct ggctggtaat
     3121 gtattcaagc gcttgccagc gcgtgagcca gcgcgaacgg ttgccgacct tcacgttgcc
     3181 tgcaacggcg cccaccttcg gatctgagaa atgccgcagc agtttgctga tggcgtccga
     3241 ctcgatttcc gtgtccgcgt cgatggtcac cacaatttcg gtgtccgcca gggacatagc
     3301 cagattcagc gccgccgctt ttccacgatt cacctgatga acaatgtgga cgcgcggctc
     3361 atgcgaaaag tttgcatcca gcaactcgcc cgttttgtcc gttgagccat cgtccacaac
     3421 gacgatccgc aagtcctgga gatccgagag caggactgag gtgacggtct gcacgatgac
     3481 gctttcttcg ttgtgcgccg ggatcagcac ggtcacgctc ggcggaggat cgggcatcac
     3541 ggcatggtcc ggccgcagct tctcgatcaa cgcgagcagg ccgatgatca cggcgcgccc
     3601 gctcaccagc acgatgccga gaatgaagat ggtcccaata agaaagcgga gccactggta
     3661 aagcgaaaag ataattccgt cgacccgcgc ttccagccgc tcttcggtgg agagcggtac
     3721 catcacttcc gcgcgcgtct ttccgatcag gtcggatacg gaaacaaact tatatccttg
     3781 cgcctgcagc gcgtcgatga tctgcggcag cgccgcgagc gtctggctgc ggtcgccgcc
     3841 gccgtcgtgc aagagaatga tgttgccttt gccgg
//
LOCUS       DASYVO010000067         4129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_129563, whole genome shotgun sequence.
ACCESSION   DASYVO010000067 DASYVO010000000
VERSION     DASYVO010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129563"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="VGR03_02180"
     CDS             complement(<1..934)
                     /locus_tag="VGR03_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV1993115.1"
                     /translation="MSDTATIASKQNPLQYVTDELKELRAKGVAPKLRVLEGEQKPVC
                     IFDGREVINLASNNYLGLTTHKALRKAALDAVKRYGAGAGAVRTIAGTMKIHMELEEQ
                     IAAFKNVEACVVFQSGFTANAGTVSAVLGKDDLIISDELNHASIIDGARLSKATIKVF
                     KHKDVKDCERILQEHASFPGKKLIITDGVFSMDGDIAPLPELCDLAEKYNCIMMVDDA
                     HASGVLGRGGRGTVDHHQCHGRVQVQVGTLSKAIGAMGGYVCGSRDLIDFLYLRARPF
                     LFSTSHPPATAAACQAAFALLDSPEGEKLVKKLWA"
     gene            complement(1035..2090)
                     /gene="tdh"
                     /locus_tag="VGR03_02185"
     CDS             complement(1035..2090)
                     /gene="tdh"
                     /locus_tag="VGR03_02185"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEV1993116.1"
                     /translation="MVGTMKALRKMQAAKGLQMDTVAVPAIGPTDVLVRVKTASICGT
                     DLHIYGWDRWSQGRIKPPVTLGHEFCGTVERVGDEVRAVKAGDFVSAEMHLNCGHCHQ
                     CRLGEAHICQSLRIIGIDQDGAFAEFVKIPASNIWKLDAAIPEHYGAILDPLGNAVHT
                     VLAGPVAGQTVLVTGCGPIGLMAIAVAKACGSSRVFATETNEKRRAMAKKMGADLVLN
                     PAAEDAVAKILVETNGTGVDALLEMSGNPTAIQQGFKALRAGGRASLLGIPTENVPLD
                     LVNDVIFKGATVQGIYGRRMYETWVQMTALLKAGRLNLEPLFGERASLDKFENAFTLL
                     QSGLAGKILLYPNGMPH"
     gene            complement(2216..>4129)
                     /locus_tag="VGR03_02190"
     CDS             complement(2216..>4129)
                     /locus_tag="VGR03_02190"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEV1993117.1"
                     /translation="GKIFYGTQRSRPAWDAEKGELSELTVVEVTIGDSRFPRKGFGVA
                     EKKIASYETEPIVLVEDSENAEVTADLVEVGGGTRESDYAGKDVKGKIVLVSAQPGAV
                     QDLAVGKFGAAGILSYAQNQKTAWWSENENLVRWGHLETFSANKTFAFMVSLKTARAM
                     KERLAKGEKITLHAVVKAGQHAGNYEVVTATIPGADAKLKEEEIAFSCHLDHQRPGAN
                     DNASGCVTILEVARTLQKLTAERKSGGCVPIGEIGKVDRALEGLGVCNWSIRPRDLAL
                     PARTIRFIFLPEIEGTLALLNGKSEFAKRIKAVIHMDMVGGGPETKAVFHVTRGPMSL
                     PSFVHDVAWAFAEFVNEESYKFAATGKAEYPMVAPEGGAEPLRAEYSAYTMGSDHDVY
                     QDSSFGIPAIYLNDWPDRYIHTNFDTAANIDPTKLKRAAFIGAASGYFLADMDESSAK
                     GALRYAEAFRLKRGTETELRTKQVTGAEAQNLRWHYLDGEIGIANSIKQIVPNSIAVS
                     EIAFEGAIPLKLSEGYKKPAGSSSGDGSVKYKRQLAPKGPLAVFGYDYFEVEWAKQAH
                     TTKPRLLEYEGLWGGGEEYAYEVLNFADGKRNAQEIRDAVSAEYGPVPLEGVVEYLRA
                     LEKIGVVEQVKRK"
BASE COUNT          828 a         1492 c          878 g          931 t
ORIGIN      
        1 tggcccacag tttcttcacc agcttctcgc cctccggcga atcgagcagc gcaaacgccg
       61 cctggcatgc cgccgccgta gccggcgggt gcgaggtcga aaagagaaat ggccgcgctc
      121 gtaaataaag aaaatcgatc aggtcccgcg agccgcaaac gtatccgccc atcgcgccaa
      181 tcgccttgct gagtgtcccg acctgaactt gcacccgccc gtggcactgg tgatggtcca
      241 ccgtcccgcg gcctccgcgg cccagtactc ccgaagcatg cgcatcatct accatcatga
      301 tgcagttgta cttttccgcg aggtcgcaca gttccggcag cggcgcgata tcgccgtcca
      361 tcgaaaaaac gccgtccgtg atgatcagct tcttccccgg aaagctagca tgctcctgca
      421 ggatgcgctc gcaatctttc acgtccttgt gcttgaaaac tttgatcgtc gctttcgaaa
      481 gccgcgcgcc gtcaatgatg gaagcgtgat tcagttcgtc ggaaataatc aaatcgtctt
      541 ttcccagaac tgccgaaacc gttcctgcgt tggccgtaaa gccagactga aacaccacac
      601 acgcttccac attcttgaac gccgcgattt gttcctccaa ttccatgtgt attttcatcg
      661 tgccagctat cgttcgaacc gcccccgctc ccgcgccata tcgtttcacc gcatccagtg
      721 cagctttgcg tagcgccttg tgcgtagtca gtcccagata attattcgaa gccaggttga
      781 tcacctctct cccgtcgaaa atgcacactg gcttctgttc gccttccagc acgcgcagct
      841 tcggcgccac acccttcgcg cgcaattcct tcagctcgtc ggttacgtac tgcaaaggat
      901 tctgcttcga tgcgattgtt gcggtatcag acatgatggc actcctcact tggactcggc
      961 tggtggaaga actattttgc gtcagctcgc ggctcagagc aaacaactcg aaggatgtgt
     1021 aaaaggcaat tcgattagtg cggcatcccg ttcgggtaca acaaaatctt cccggccagg
     1081 ccactctgca acaatgtgaa cgcgttttca aatttgtcca gcgatgcccg ctcgccgaaa
     1141 agtggctcca gattcaatcg acccgccttt agcagcgcgg tcatctgcac ccaggtctca
     1201 tacattcgcc gcccgtaaat cccctgcacc gtcgcgccct tgaaaatcac gtcgtttacc
     1261 agatccagcg gcacattctc cgtcggaatc cccagcaacg acgcgcgccc gcccgcccgc
     1321 agcgccttga atccctgctg aattgccgtg ggattcccgg acatttccaa cagcgcgtca
     1381 actcctgtgc cgtttgtttc cacaagaatt tttgcaactg catcttccgc cgccggattc
     1441 aacaccaagt ccgcgcccat ttttttcgcc atcgcgcgcc gcttttcgtt cgtctccgtc
     1501 gcaaagaccc tcgagctgcc gcaagccttc gccacagcga tggccatcaa cccaatcggc
     1561 ccgcagcccg taaccaaaac cgtttgcccg gcgaccggcc ccgccagcac cgtatgtacc
     1621 gcgttgccca gcggatcgag aatcgccccg taatgctcgg gaatcgccgc atctagcttc
     1681 cagatattcg acgccggaat cttcacaaat tccgcgaacg cgccgtcctg atcgatcccg
     1741 ataatgcgca ggctttggca gatgtgcgct tcgcccagcc ggcactggtg gcagtgcccg
     1801 caattgaggt gcatttccgc gctgacgaaa tctcccgcct tgaccgcgcg cacctcgtcg
     1861 cccacccgct cgaccgtgcc gcaaaattca tgccccagcg tcaccggcgg cttgatgcgc
     1921 ccctgcgacc agcggtccca cccataaatg tgcaaatccg tcccgcaaat cgaagccgtc
     1981 ttcacccgca ccagcacgtc cgtcggcccg atcgccggca ccgcgaccgt gtccatctgc
     2041 agacccttcg ccgcctgcat cttccgcaac gccttcatcg tcccgaccat ctacctcgtc
     2101 cctctctcac cgcacaaacc ccgatgctag cacaccagta gcacagactt cagtctgtgc
     2161 cctgcaaacc ccgctgcggc ctttcgtttc acctcgaatt tccaatttcg attttctatt
     2221 ttcgcttcac ctgctccacc actccaatct tctccaacgc cctcaaatac tccaccaccc
     2281 cttccagcgg caccggccca tattccgccg acaccgcatc gcgtatctcc tgcgcattcc
     2341 tcttcccatc cgcaaaattc aacacctcat acgcatactc ttccccgccg ccccataacc
     2401 cttcatactc cagcaatctt ggtttcgtcg tgtgcgcttg tttcgcccat tccacctcga
     2461 aatagtcgta cccaaacacc gccaacgggc ctttcggcgc tagctggcgc ttatacttca
     2521 ccgaaccgtc tccgcttgag gaacccgcag gcttcttgta gccttcgctt agtttcagtg
     2581 gaatcgcgcc ttcgaacgca atctccgaaa ccgcaataga atttggaacg atctgcttga
     2641 tcgaattcgc aattccaatt tcgccatcca aatagtgcca cctaagattc tgggcctctg
     2701 cacccgtgac ctgtttcgtg cgaagttcgg tttcggttcc gcgcttaaga cggaatgcct
     2761 ccgcgtaacg aagcgctcct ttcgcgctac tctcatccat atccgccaaa aaatacccgc
     2821 tcgctgcccc gataaacgcc gccctcttga gcttcgtcgg atcgatattc gcggccgtat
     2881 cgaaattcgt atgaatgtag cggtccggcc agtcattcaa ataaatcgcc ggtatcccga
     2941 acgacgaatc ctgatacaca tcatgatcgc tccccatcgt gtacgccgaa tactccgcgc
     3001 gcagcggctc cgcgccgcct tccggtgcca ccatcggata ttccgccttc cccgtcgccg
     3061 caaatttgta cgactcctca ttcacaaact ctgcaaacgc ccacgccacg tcatgcacaa
     3121 agctcggcaa gctcatcggc ccgcgtgtca cgtggaacac cgccttcgtc tccggccccc
     3181 cgcccaccat gtccatgtga atgacggctt tgatacgttt ggcaaattcg gactttccgt
     3241 taagaagcgc gagcgttcct tcaatttccg gaagaaaaat aaatcgaatc gtccgcgccg
     3301 gcagggccaa gtctctcggt ctgatagacc agttgcaaac cccaagtcct tcaagcgcgc
     3361 ggtcaacctt gcctatctcc ccaattggca cacacccgcc cgatttgcgt tctgcagtta
     3421 acttctgcag cgtccgcgcc acctcaagaa tcgtcacgca cccgctcgca ttgtcattcg
     3481 ccccgggccg ctgatggtcg aggtggcacg aaaacgcgat ctcttcctcc ttcaacttcg
     3541 catccgcccc cggaatcgtc gccgtcacca cctcataatt ccccgcatgc tgccccgcct
     3601 tcacaacggc atgcagcgtg atcttttctc ccttcgccag ccgttccttc atcgcccgtg
     3661 ccgtcttcag cgacaccatg aacgcaaacg tcttgttcgc cgaaaatgtc tccaggtgtc
     3721 cccagcgaac taaattctca ttttcactcc accaagccgt cttctggttc tgcgcatagc
     3781 tcaaaattcc cgccgccccg aatttcccca ccgccagatc ctgcaccgct cccggctgcg
     3841 cgctcaccag cacaatcttt cctttcacat ctttgcccgc gtagtcgctc tccctagttc
     3901 cccctcccac ttccaccaaa tccgccgtca cttccgcgtt ctcgctgtcc tcgaccaaca
     3961 caatcggctc cgtctcataa ctcgcaatct tcttctcagc tacaccgaac cccttccgtg
     4021 ggaaacgaga gtctcctatc gtcacttcta ctaccgtaag ctccgacaat tctccctttt
     4081 ccgcatccca agccggccgc gaccgctgcg tcccataaaa aatcttccc
//
LOCUS       DASYVO010000068         8599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_1243, whole genome shotgun sequence.
ACCESSION   DASYVO010000068 DASYVO010000000
VERSION     DASYVO010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8599
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VGR03_02195"
     CDS             complement(<1..718)
                     /locus_tag="VGR03_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV1993118.1"
                     /translation="MKSFTGKALRWEWREGVIDLTLDHEPANEIGTAMLSELEEFVAA
                     FGVLAPETSVCIIASARKSVFSAGADLRELYHGAVALPGKERLTGVRDFLERIHAVLN
                     AIDAAPFVTIAAIHGVCFGGGLELALACDIIIADKMARFAFPELRLGLIPGFGGIPRL
                     KRDLGNAFVRDLLFTGRTVNATRAHAAGLVAQLVGEGEALRIARSTAAQITKFDAVTR
                     AAAKKFIKPIPYDELRREIDL"
     gene            complement(715..2427)
                     /locus_tag="VGR03_02200"
     CDS             complement(715..2427)
                     /locus_tag="VGR03_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV1993119.1"
                     /translation="MPNFLEMILAQLKRADGRVVLREICEDRFVSVTGRELLDQIQLV
                     RTFIRSSGVQLGDRCVLLAPNSVRWAAFHLALMAEGVIVVPLYSRQAPAELVGMLKDC
                     QPRLIFVGEAALGEGVAQAWPDAPRRVLFDDVLRQSAQQPRLPDAPNPRPDSDIVTII
                     YTSGTSGEPKGVCLNVGNLTHMLSCTTERLDQLMGATHEPERVFHYLPFNFAASTILM
                     LSCLARESVLTLSTDLNKLADEIRLAAPNYFLNVPTLPERVRRGVEEAIAKRPAAIRS
                     LFAKARSAWRRKHVGRGRALDAFWLALGRALIFRKIRERFGPHLRALICGSAPLAPET
                     QQFFLMLGIPVLQAYGLTETTGICTLDDPRVPVEPGYVGTVISGIEMKLADNEEIVVR
                     GPHIFPGYWNRPEETARVLQDGWFHTGDQGEVNVRGNWRISGRIKNLIILNSGHNIAP
                     EPIEEKVARLLPAAQQVVVVGNGRGYLCALVTGEIESAAVQAALDAVNPELPHYRQIR
                     KFTIVREAFTPEIGLLTANGKLRRDAINARYASEIKVMYDATGNREAAMSSGNRKVIG
                     GQQA"
     gene            complement(2434..3873)
                     /locus_tag="VGR03_02205"
     CDS             complement(2434..3873)
                     /locus_tag="VGR03_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993120.1"
                     /translation="MTDFLLVAWVSALIELTRFGWMLGLGERWTPGEKLKLLFAGYNG
                     TRNTGSDVRVQEMLRQVRHVLGAEHVDFSVMTQSFERTKGYFEGTRQVHLPDVYPPFL
                     YREVRQNHGVIACEGSMFKSKFANALTTMMIGSLGLASAENKLSLAYGGEAGHMDGII
                     QSMCERYVHDSLVITRNVESRTLLSSLGIPTELGTDTAWTFEPHPPEYGRKALRDAGW
                     DEKTPVLVLCPIHPFVWPVKASIAKYLARATTGAYKDSQYRTVYFFESGAEVDRKYQH
                     YISGFTKATQAFVQRHRVFPILVAMERLDAVACREIAARIPGTPVFTPDDYDMFQLVS
                     ILRAGTYMVSSRYHGIVTCMPSLVASAGVTMDERIRNLMRERGHQHLLLTVDDPDLEP
                     KLLQVMETLVAEADSVREGIGQTVVRNLKVMARMGTFLEDVVRRTYPEFPLRSGVFSW
                     EEYLPPLSKNLIQLAERYDSSPAALAAVR"
     gene            complement(3870..5027)
                     /locus_tag="VGR03_02210"
     CDS             complement(3870..5027)
                     /locus_tag="VGR03_02210"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1993121.1"
                     /translation="MAIFVTGSTGYLGSYLAAGLLTEHRDKLNLLIRAKTEQEARERL
                     WTSLQLHFEFPEFAEYLNTRVRIFRGDLTSERFGLSDDEYHALVDTTDSLIHCAASLN
                     RKSEKQCLNVNLRGTLEVIQLARRAQNRNGLRRYSHVSTVAVAGKRKNEIVTEDASID
                     WSRSDYDPYARTKKFCEHMANQLLPDVPKTIFRPAIVMGDSRRPETSQFDMVQAFHVL
                     AQMPVLPLSPHDKIDIVPANYVGKAVVTIHQKEAPAHGIYHLSSGTGSQTYQELTDAI
                     SQAGGMLRPMFLPSLGGPFSSTVNWVANRSGAAGYGASLLKVFWPYLDWNTVFDNARV
                     VAELGETPAKFSSYAAPLLKYSRENKFQFPAKPWPSVPGAEKNAAAGRARA"
     gene            complement(5012..7390)
                     /locus_tag="VGR03_02215"
     CDS             complement(5012..7390)
                     /locus_tag="VGR03_02215"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1993122.1"
                     /translation="MAQPGSNSKGVLPAAGNGNVSYWRVEGSLLELGALRPVGFFTWN
                     SQSFAQRWARRAGMAGMALARPFAYFTSRTFATRFLHTLLRGVSRDRLDLLGEEYFHY
                     VLKPQLRRDAAEKLIEAVRNGERVVLVGQLLENVLRPLAHHFGVESFISNRLEFRDGK
                     ATGRLLDPIVRPRGPLAWIASGSADGRITREKLLNQLGWTGKPEQVESAVETTARPTI
                     PPGKSVALFGDTPRAGRLSVRENLAHRHLLLIGVTGFIGKVWLVDLLENIPNIGKITL
                     LIRRNRTTSAQRRFEKIVEESPTFDTLQERHGRKLGAFLEEKVEVVEGDVSLPSLGLD
                     EATQARLASSLDLIVNSAGLTDFNPDLRDAVSSNVDSAINLLDFLRKCSHAGLIHLST
                     CYVVGMRDGRVTEQLQDNYNPANDSQFDAAREVDSLRETIRHVVERAESPELDKALLR
                     QALGRGGNEAFAPARELDGVLRRNRARWARNRLVRVGLRRAQHLGWPNTYTFTKSLGE
                     SILARRGGDLPIAIVRPSIVESSERSPFTGWNEGINTSGPLSYLLGTNFRQLPSNERK
                     CLDVIPVDMVCRGMTLIASAVIARRHARMYQLATSAINPVNMGRSIELTGLAHRKHYR
                     TTNGIDSWLKVKFESIPVSKQRYERLSIPMQKAVISSINRAAISLHMKKPPLARAERD
                     LIRAEKLIELYEPFILHNEHVFECENARLLSAALPPEERPLFDFAPESIDWWDYWINV
                     HIPALRRWCYPLMEGRPLESREPRALDWSGEATRGAALSKAAAPSDPLWRSS"
     gene            complement(7690..>8599)
                     /gene="leuB"
                     /locus_tag="VGR03_02220"
     CDS             complement(7690..>8599)
                     /gene="leuB"
                     /locus_tag="VGR03_02220"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEV1993123.1"
                     /translation="DAVFLGAVGGPKWDALPVGKRPESGLLDLRKGLGLYVNVRPVKL
                     LEPLRGISPLKADRLGDLDLEIVRELAGGMYFGERGNTTEKGVERAWDTESYSTPEIE
                     RIATFAYARAENRSRRLCSVDKANVLTSSQLWRRTVTAMSAVYPAVKLEHMYVDNAAM
                     QLTLRPAQFDVMLSSNMFGDILSDAAAGLVGSIGLIPSASFGTSAPLFEPIHGSAPSL
                     AGTDSADPIGSILSATMMLRDAFGLSLEADWVEQSLQRVLAGGYRTPDITEPGARAVG
                     GSVFTEILREEMQRTFEHAERYGWGV"
BASE COUNT         1790 a         2516 c         2671 g         1622 t
ORIGIN      
        1 agaggtcgat ctcgcggcga agctcatcgt atgggatcgg cttgatgaat ttcttggcgg
       61 ccgcgcgtgt taccgcgtca aatttcgtga tttgcgcggc ggtcgagcga gcaatgcgga
      121 gggcttcgcc ttcaccgaca agttgcgcca ccaggccggc ggcatgtgcg cgagtcgcgt
      181 tgaccgttcg cccggtgaaa agcaaatccc tgacgaaagc attgccaaga tcgcgtttca
      241 agcgtgggat acccccgaag ccggggatga gtcccaggcg cagctcgggg aacgcgaacc
      301 gcgccatttt gtcggcgatg ataatgtcgc aagccaaggc gagctcgagt cctccgccaa
      361 agcagacgcc gtgaatggcg gcgatcgtca cgaacggtgc agcatcaatc gcgttgagaa
      421 cggcatgaat gcgttccagg aaatcgcgaa cacctgtgag acgttctttt ccaggaagtg
      481 ccacggcacc gtgataaagc tcgcgcaggt ccgcaccggc ggaaaaaaca gacttgcgcg
      541 cgctcgcaat aatgcagacg gaagtctccg gcgcaagcac accaaatgcg gcaacaaact
      601 cttccagctc cgagagcatg gccgtgccaa tttcattggc aggttcatga tcgagcgtca
      661 gatcgatcac cccttcgcgc cattcccaac gcaacgcttt gccagtgaag cttttcatgc
      721 ttgctgcccg ccgattacct tgcgattgcc actggacatc gccgcttcgc gattcccggt
      781 ggcatcgtac atgaccttga tttcggaagc atagcgcgca ttgatggcgt cgcggcgaag
      841 tttgccgttg gcggtgagta gcccgatctc aggggtaaag gcttcgcgga caatcgtgaa
      901 ctttctgatc tggcggtaat gagggagttc gggattcaca gcatcgagcg ccgcctgaac
      961 ggccgcggat tcaatctccc cggtcacgag cgcgcaaaga tagccgcgcc catttccgac
     1021 cacgacgacc tgctgcgccg cgggaagaag ccgcgcgact ttttcctcga tcggctcggg
     1081 cgcaatgttg tgcccggaat tcaagatgat caggttcttg atgcggccgc tgatgcgcca
     1141 attcccgcga acgttgactt cgccctgatc gccggtgtgg aaccatccgt cttgcagcac
     1201 gcgggcagtt tcttctgggc ggttccagta gccggggaaa atgtgcgggc cgcgcacaac
     1261 gatctcttca ttatcagcca gcttcatttc gattccgcta atcaccgtgc caacgtagcc
     1321 cggctcgacg ggcacgcgcg ggtcgtccag cgtgcaaatg ccggtggttt ccgtgagccc
     1381 gtaggcctgc agcacgggaa tgccaagcat caggaaaaac tgctgcgtct ccggagcgag
     1441 cggcgctgaa ccgcagatca gcgcgcgcag gtgtgggccg aagcgttcgc gaatcttgcg
     1501 aaagataagg gcgcggccga gcgcgagcca aaacgcatcg agggcgcggc cgcggccaac
     1561 gtgcttgcgc cgccaggcgc tgcgtgcctt cgcaaacaag gaacggatcg cggccggccg
     1621 ctttgcgatg gcctcctcga cgccacgccg gacacgctcc ggcagcgtgg gtacgttcag
     1681 gaaataattc ggtgctgcca ggcggatttc gtctgcaagc ttgttgaggt ccgtcgaaag
     1741 tgtaagaacg ctttcgcgtg cgaggcagga cagcatcaag atcgtagaag ccgcaaaatt
     1801 gaacggcagg tagtggaaaa cgcgttccgg ttcgtgtgtc gcacccatca gttgatcgag
     1861 gcgctccgtg gtgcaggaaa gcatgtgcgt gaggttcccg acgttcaggc aaacaccctt
     1921 cggctccccc gaagttcccg aagtatagat gatggtgacg atatcgctgt ccgggcgagg
     1981 attgggcgca tcgggaagcc gaggttgctg cgcggattgg cgcagaacat catcgaaaag
     2041 cacacggcgc ggagcgtcgg gccaagcttg ggccacgcct tcgccgagtg cggcttcgcc
     2101 cacaaaaatc agacgcggct ggcaatcctt cagcatgccg acaagttcgg cgggcgcctg
     2161 ccgcgaatag agaggcacga cgatcactcc ctcggccatg agcgccaaat ggaaagccgc
     2221 ccagcgaacg gaattgggcg caagaagaac gcaacggtcc ccaagttgga cgccggagct
     2281 tcgaatgaac gtacggacaa gctgaatctg gtcgagcaat tccctgcccg tcacactcac
     2341 aaagcgatcc tcgcaaattt cgcggagaac gacgcggcca tcggcgcgct tcagttgagc
     2401 aagaatcatt tcgaggaaat tgggcatgaa gaatcagcga accgccgcga gcgcggccgg
     2461 cgaagagtcg taacgctcgg cgagttgaat caaattcttg ctcagcggcg gcagatactc
     2521 ttcccaggaa aaaacgccgg agcgcagcgg aaattcagga taggtgcgcc ggacaacatc
     2581 ttcaagaaac gtgcccatgc gggccatcac tttgagattg cggaccacgg tttggccgat
     2641 gccttcgcgg acggaatcgg cttccgcgac cagcgtttcc atgacctgga gaagcttcgg
     2701 ctcgagatcg gggtcgtcga cggtcagcaa gagatgctga tgtccgcgtt cgcgcatcag
     2761 attgcgaatg cgctcgtcca tggttacgcc cgccgaggcc acgaggctgg gcatgcaggt
     2821 cacaatgccg tggtagcgcg atgaaaccat ataggtgccc gcgcgcagga tcgaaacaag
     2881 ctggaacata tcataatcat ccggagtgaa aaccggtgtg cctggaatcc gtgcagcgat
     2941 ttcgcggcag gcgaccgcgt caagccgttc cattgccact agaatcggaa aaacgcgatg
     3001 gcgctgaacg aacgcctgcg tcgccttggt gaatccagaa atgtaatgct ggtatttacg
     3061 gtctacttca gcgccgcttt caaaaaaata aacggtgcgg tactggctgt ccttatacgc
     3121 gccagtagtt gcacgcgcga gatacttcgc aatggaagct ttcaccggcc agacaaaagg
     3181 atgaatcggg cacaagacga ggaccggagt cttttcgtcc cagccggcgt cgcgcagcgc
     3241 ctttcggcca tattccggag gatgaggttc aaacgtccag gccgtatcgg tgccaagctc
     3301 tgtcgggatt ccgagcgagc tcaatagcgt ccgcgattcc acgtttcgcg tgatgaccag
     3361 tgaatcatgg acgtagcgct cgcacatgga ttgaatgatc ccgtccatgt ggccagcttc
     3421 gcccccgtaa gccagtgaga gcttgttttc agccgaggcc aggcccagcg aaccgatcat
     3481 catcgtcgtc agtgcgttgg caaacttgct cttgaacatc gagccttcgc aagcgatgac
     3541 gccatgattc tgccgcacct cgcgatacag aaaaggcggg taaacgtcgg gaaggtgaac
     3601 ctggcgggtg ccttcgaagt agcctttggt gcgttcgaag ctttgcgtca tcacgctgaa
     3661 atcgacgtgc tccgcgccga gaacatgccg aacctgccgc aacatctcct gaacgcgcac
     3721 atcgctgccg gtgttgcgcg tgccgttgta gcccgcgaag agcagcttca gtttttcacc
     3781 cggcgtccag cgttcgccga ggcccagcat ccagccgaag cgcgtgagct caatcagcgc
     3841 gctaacccag gcgaccagaa gaaagtccgt catgcgcggg ctctcccggc cgcggcattt
     3901 ttctcggcgc ccggaacgga aggccagggc ttggcgggaa actggaattt attttcgcgg
     3961 ctgtatttca aaagaggggc tgcataactc gagaattttg cgggcgtttc gccgagctcc
     4021 gcgacgacgc gcgcattgtc gaaaacggtg ttccagtcga gatagggcca gaaaactttg
     4081 agcagcgagg caccgtatcc ggcagcgccg ctgcgattcg caacccaatt gacggtgctg
     4141 gaaaaaggtc cgccaagaga aggcaaaaac attggccgaa gcatgccccc ggcttgcgaa
     4201 attgcatccg tcagttcctg gtacgtctgc gaacctgtcc cggaggacaa atggtagatg
     4261 ccgtgcgcgg gcgcctcttt ttgatgaatc gtcacgaccg ctttgccaac atagttcgcc
     4321 ggtacgatgt cgattttatc gtgaggactc aggggaagaa caggcatctg cgccaaaacg
     4381 tggaacgctt gaaccatatc gaattgcgaa gtctccggtc ggcggctgtc gcccataacg
     4441 atcgcggggc gaaaaatcgt ctttggcaca tcgggcaaaa gctgattcgc catgtgctcg
     4501 cagaatttct tggtgcgcgc gtagggatcg taatccgagc gcgaccagtc gatggaggcg
     4561 tcttcggtta caatctcgtt ttttctcttc cccgcgacgg caacggtgga gacgtgcgaa
     4621 taacggcgca ggccgttgcg attctgcgcg cggcgcgcga gttgaatgac ttcgagtgtg
     4681 cctcgcaggt tgacgttcag gcactgcttc tccgatttgc gattcagcga cgcggcacag
     4741 tgaatcaggg agtccgtggt gtccaccagc gcgtggtatt cgtcatcgga gaggccaaaa
     4801 cgctcgctgg tcaggtcgcc gcgaaaaatg cgcacgcgag tgttcagata ttcggcgaat
     4861 tcgggaaact cgaaatgaag ctgcaacgaa gtccacagcc gctcgcgcgc ttcctgttcg
     4921 gtcttggcgc gaatcagaag attcagcttg tcgcggtgct cggtgaggag ccccgcggca
     4981 agataactgc ccaggtagcc ggtcgagccg gttacgaaga tcgccataaa gggtcggagg
     5041 gtgccgcggc tttggagagc gccgccccgc gcgtggcttc gccggaccaa tccagcgcgc
     5101 gcggctcgcg cgattcgagc gggcggcctt ccatcagcgg atagcaccag cggcgcagcg
     5161 ccggaatgtg cacgttgatc cagtaatccc accagtcgat cgattccggc gcgaagtcga
     5221 agagtggacg ctcctcgggc ggcagcgcgg cggagagcag gcgcgcattt tcgcattcga
     5281 agacgtgctc gttgtgaaga atgaaaggct cgtagagctc gatcagcttc tcggcgcgga
     5341 tcaagtcccg ctccgcccgg gcgagcggtg gcttcttcat gtgaagggaa atggcggcgc
     5401 gattgatact ggagatcacg gccttctgca tcggaatcga gaggcgctcg tagcgctgct
     5461 tcgagacggg gatggattca aattttactt ttagccagct atcgataccg tttgtcgtgc
     5521 ggtagtgctt gcggtgggca agtcccgtga gttcgatgct ccgccccatg ttgacgggat
     5581 tgatcgcgga agtcgcaagc tggtacatgc gggcgtggcg ccgcgcgata accgcggaag
     5641 caatgagggt cattccgcgg cagaccatgt cgacgggaat gacgtcgaga catttccgct
     5701 cattcgaagg aagctggcgg aaattcgtgc cgagaaggta ggagagcggc ccggacgtat
     5761 tgatcccttc attccacccg gtgaaggggg agcgctccga gctttccacg atcgaggggc
     5821 gcacgatggc gatcgggaga tcgccgccgc ggcgcgcgag gatcgactcg ccaagactct
     5881 tcgtgaaagt gtaagtattc ggccagccca gatgctgcgc gcggcgcagg ccgacgcgca
     5941 cgagccggtt gcgcgcccag cgggcgcgat tgcgtcttag aacaccgtcg agctctcggg
     6001 cgggcgcgaa agcttcgttg ccgccgcgtc ccagcgcttg gcgaagaagc gccttgtcga
     6061 gttccggact ttcggcgcgt tccacaacgt gacgaatcgt ttcgcgtagt gaatcgactt
     6121 cgcgcgcggc atcgaattgg gaatcgttgg cgggattgta attgtcctgc aactgttcag
     6181 tgacacggcc gtcgcgcatg ccgaccacgt agcaggtgga aagatgtatc agcccggcgt
     6241 gactgcactt tcgcagaaag tcgagcaagt taattgccga atcgacgttg gaagagaccg
     6301 catcgcgcaa atcaggattg aaatccgtca ggccggcgct gttcacgata aggtcgagtg
     6361 aacttgccag gcgcgcttgc gtggcctcgt caagccccag gcttggcagg ctcacgtccc
     6421 cttcaacgac ctccactttt tcttccagaa atgcaccgag cttccggcca tggcgctcct
     6481 ggagcgtgtc gaaggtcgga gattcctcga cgatcttttc gaagcgtcgc tgcgcggacg
     6541 ttgtccggtt gcggcgaatc aagagcgtga tcttgccgat gttgggaata ttttcaagca
     6601 gatcgaccag ccagaccttg ccgataaatc cagttacgcc aatcagcaag aggtggcggt
     6661 gcgccaggtt ctcacgcacc gagagacgac cggctcgcgg agtgtccccg aatagcgcga
     6721 cggacttgcc cggtggaatg gttggacggg cggtcgtctc gaccgcgctt tccacctgct
     6781 cgggcttccc cgtccagcca agctggttca gaagtttttc ccgcgtgatc cggccgtctg
     6841 cgctgccgct cgcgatccag gccaagggac cgcgcggacg cacgatggga tcgagcagcc
     6901 gtcccgtcgc tttgccgtcg cgaaattcca ggcggttcga aatgaacgac tcaactccga
     6961 aatggtgcgc cagcggccga agtacgtttt ccaggagttg cccgaccagg acgacacgct
     7021 cgccgttgcg gaccgcttcg atcagcttct cggcagcatc gcggcgcaat tgcggcttca
     7081 ggacatagtg aaagtactct tcgccgagga gatccaggcg atcgcggctg acgccgcgaa
     7141 gcaaagtgtg caagaagcgt gtggcaaacg tacggctcgt gaaataagcg aaaggccgcg
     7201 ccagcgccat gcctgccatg ccggcgcggc gcgcccagcg ctgggcaaag ctctgcgagt
     7261 tccaggtgaa aaaaccgacc gggcggaggg cgccgagttc gagcagggag ccttcgacgc
     7321 gccaatagga aacattgccg ttcccagcag caggcaagac acccttcgaa ttggatccgg
     7381 gttgcgccat agcgggccta cgacgaatgg cgaccgatcc gccgcgagga tagctcaaga
     7441 aaatggcgat gagtaatgat gtttctcacc ttctggtcaa gctgctctgg aacggactcg
     7501 tagcccgcgc cttcatccac acggccgtgt ggctcccgac tctggccgat cgcaggctgc
     7561 ttcggaaaat gtaaaagtag cattctcagc ggcttctcgc aaggcaaccg ggcaggcggt
     7621 ggcagtgcgg ctcggagcgg gggcccgata gtgatgaaaa agcacaaggt gagcggttgg
     7681 gaggtgactc tagacgcccc agccgtagcg ctcggcgtgt tcgaaggtgc gctgcatctc
     7741 ttcccgcagg atttcggtaa agacggagcc gcccacggcg cgcgcaccgg gctcggtgat
     7801 gtcgggcgtc cgatatccgc cagcgagtac ccgttgcagc gactgctcga cccaatccgc
     7861 ttccagggag aggccgaagg cgtcgcgcag catcatcgtc gcgctaagaa tcgagccaat
     7921 cggatcggcg gagtccgtcc cggccaggga cggtgccgaa ccatgaatgg gctcaaaaag
     7981 cggcgcggac gtaccaaaac tagccgatgg aatcagtccg attgagccga ccagccccgc
     8041 cgccgcatcg ctcaaaatat cgccgaacat gttgctgctc agcatcacgt caaactgcgc
     8101 cggccgcaga gtcaactgca tcgccgcgtt gtcgacatac atgtgctcga gtttcaccgc
     8161 gggatagacc gcgctcatgg ccgtgacggt acggcgccag agctgcgagc tggtcaacac
     8221 gttggctttg tccaccgagc acagccgccg cgagcggttc tccgcgcgcg cataggcaaa
     8281 cgtggcgatg cgctcgatct ccggtgtcga ataggactct gtgtcccagg cgcgctccac
     8341 gcctttttcg gtggtgttcc cgcgctcgcc gaaatacatt ccgccggcga gttcgcgcac
     8401 gatctcgaga tccagatctc caagccgatc cgccttcaag ggcgagattc ctctcagcgg
     8461 ctcgagcagc ttcacgggac gaacgtttac gtagagcccc agcccttttc gaagatcgag
     8521 cagtccgctc tccgggcgct tcccgacggg cagggcatcc cacttcggcc cgcccaccgc
     8581 tcccagaaaa acggcgtcg
//
LOCUS       DASYVO010000069         9449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_86328, whole genome shotgun sequence.
ACCESSION   DASYVO010000069 DASYVO010000000
VERSION     DASYVO010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9449
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86328"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1175
                     /locus_tag="VGR03_02225"
     CDS             183..1175
                     /locus_tag="VGR03_02225"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV1993124.1"
                     /translation="MSYNFRDLLALEPLTADELGFLLDQSKPFQEIQKHPLKKLATLR
                     GKTVALAFFETSTRTRISFGTAASRLGADSMNLQAEASSLKKGETLLDTVFNLNAMKP
                     DCLVMRHAASGAPHYVARHLDIPVVNAGDGTHEHPSQGLLDALTIRDRLGTIKNLNVT
                     ILGDILHSRVARSNIHLLGKFGCRFTLCGPAMWVPRELEKIAPGTAIRCVYRIEEALE
                     GAHVIMMLRVQTERLNDPPLSANDYILLYQLNAERLRRANRDAIVLHPGPINRGIEIT
                     PEVADGPQSCVLEQVTNGVAVRMAILYLLISGATETDTHPALEVKPKESANASD"
     gene            1162..2436
                     /locus_tag="VGR03_02230"
     CDS             1162..2436
                     /locus_tag="VGR03_02230"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV1993125.1"
                     /translation="MLLIKNGRVVDPASKTDAALDVLLDGEKIKEVGPAGKVAAPQGT
                     EIFDASGLIVAPGFIDIHVHLREPGQESSETIETGTRAAARGGFTAVCCMPNTKPVND
                     NASVTRFILDRAKVGGSVRVWPIGAASVGSKGEAIAEIAAMKEAGIVGVSDDGKPIAT
                     AKLARQVMDYCRSLDLPVIEHAEDVSLAAGAVMREGVTSTRLGLRGMPAAAESVCVAR
                     DVQLAELTGARLHVAHLSAKASLEQVRWAKSRGLRVSCEVTPHHFTLIDEDVQYDSKF
                     KMNPPLAAREDREALLAGLADGTVDAIATDHAPHEPALKDVEFDRAPFGILGFETALA
                     LALEQLVPSGRLSLMRVVELFTTGPARVLGIERNITAGQPADLTIFSIDRAWTYSVKE
                     SPSKSRNSPFDGRAFKGAAVATIVAGRVVHKQ"
     gene            complement(2507..3391)
                     /gene="thiD"
                     /locus_tag="VGR03_02235"
     CDS             complement(2507..3391)
                     /gene="thiD"
                     /locus_tag="VGR03_02235"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1993126.1"
                     /translation="MSTNGHSQSAPPILLTIAGFDPSCGAGTAADLKTFAAHGCYGVA
                     AITSLTVQNTQGVETVHNTPSAELRAQLEVLAKDCEIAAVKIGMLGNRGNAVVVAEFL
                     DAHKFAHVVHDPVMKSSSGAELLDAGGINFVAAELLKRASVITPNVPEAEVLTGLTIK
                     DVADMEAAARKLVELGARAVIVKGGHMERAVDILFDGTEMVQLGGDRVKTDNTHGTGC
                     TFASALTAQLAAGRGLLEAATLAKAYVTKAIENGYAIGKGRVPLDHFYRQRIEPPARG
                     IHEVPQHGMHPAAEPAAH"
     gene            3699..5360
                     /locus_tag="VGR03_02240"
     CDS             3699..5360
                     /locus_tag="VGR03_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV1993127.1"
                     /translation="MWNSRALGAPWHQQPGAAPAEGSDPPAQSGDATARPKRSIASGP
                     FQPTWDSLKQYQTPDWFRDAKFGIWAHWTAQCVPEQGDWYARLMYIQGLRQNQYHVEH
                     YGPPSQFGFKDIDNLWKADKWEPDALMQLYVKAGARYFVSLANHHDNFDCYDSMHHEW
                     NSVRVGPNKDIVGIWAKTARKHGLRFGVTNHSAHAWHWFQTAYGYDPEGPLAGVPYDG
                     FLNQEDGKGKWWEGLDPQNLYAGPNMVMPKGFTTSKAARDWHEQHDRIWTEDPPANNP
                     AFVEKWFLRCQDLLDRYSPDLLYFDNTELPLGQAGLDIAAHFYNSNMRNHQDRLEAVL
                     NSKELRPDHAGTMVLDIERGRANKILSAPWQTDTCIGDWHYRRSLYEKHEYKTAETVI
                     KVLVDIVSKNGNLLLNIPLRGDGSIDDDERGVLAELATWMPDNQEAIFGTRPFVVFGE
                     GPPDLASTGNFNESKGRPYTAEDIRFTTKGETLYAIALGWPANGKIQINTLARGSANY
                     PREVGRVELLGTKGALPFTRDGKGLTVTLPEKKPNDYAYALRITL"
     gene            complement(5471..6739)
                     /locus_tag="VGR03_02245"
     CDS             complement(5471..6739)
                     /locus_tag="VGR03_02245"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEV1993128.1"
                     /translation="MVPRIAPREDLNPFRIAQIQFDMAAEFLKLDPGLRQILRTPKRV
                     LEVSLPTKMDNGQVKVFTGYRVQHNVSRGPGKGGIRFHPNVTLDEVKALAMWMTWKTA
                     AVNVPFGGAKGGVICDPKRMSKSELERMTRRYASEILPIIGPSQDIPAPDVYTDAQTM
                     AWIMDTYAMTVGHSAHGVVTGKPVSIGGSEGRLEATARGLLYVVEEACKLKKISLRGA
                     TVAIQGFGNAGATAARLFAEKKAKIVALSDTRGGVTNTRGIDPIKAIRYKERSGTVVG
                     MPGASRITNDELLTMKCDILIPAALENVITLHNAEAVKARIVAEAANGPTTPHADEIL
                     ARRGVIVLPDILANAGGVTVSYFEWVQNQEGLMWDVEEVNARLQKIMTRAFHEMVDTM
                     RKHHVPPRAGAMILAVGRVSEATLVRGLFP"
     gene            complement(6883..7200)
                     /locus_tag="VGR03_02250"
     CDS             complement(6883..7200)
                     /locus_tag="VGR03_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993129.1"
                     /translation="MNFLARVVRFLFWLLVVSWGVRLLRRIVGSMLRNEAAPGPQTAD
                     ASPAAQTSAAARRLVRDPVCGMHVDETLSIPLREAGELLHFCSPACRDAYAINTRKFA
                     ANG"
     gene            complement(7470..8747)
                     /gene="serS"
                     /locus_tag="VGR03_02255"
     CDS             complement(7470..8747)
                     /gene="serS"
                     /locus_tag="VGR03_02255"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV1993130.1"
                     /translation="MHDLNYFRDHLDVFADMAKKRGIALDLDAYRALDKERRELITAN
                     EQRKAQRNKASEEIASLKKEKQDAGTLIAEMKQVSERIKQSDERIAELDTKQRDLLLT
                     IPNVPHSSVPFGHFVTDNFEVRRWGNPPKFDFAPTPHWDVGERAGILDLAAATKITGA
                     RFAVYKGWGARLERALANFFLDVHTREHGYTEILPPFLVNTASLTGAGQLPKFAADLF
                     KIQDTDFWLTPTAEVELHNLYRDETIPEEKLPIYVTAWTACFRSEAGAAGKDTRGILR
                     QHQFQKVELFKFTHPEKSYEEHEALVCNAETILQKLGLHYRTVLLCTGDMGFASSKTY
                     DIEVWLPASNEFREISSCSNCEAFQARRAGIRFRPKGGGKSDFVHTLNGSGLAVGRTW
                     IAVVENYQQADGSILIPEVLRPYMGIDRIPAAT"
     gene            complement(8823..9218)
                     /locus_tag="VGR03_02260"
     CDS             complement(8823..9218)
                     /locus_tag="VGR03_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1993131.1"
                     /translation="MSGTPANLGITNIGQICINVQDLQRATAFYRDVLGLPLLFTVPN
                     LAFFDCAGVRLMLGRAETPEFDHPSSILYFRVPDLNAAYQRLVELSVQIFAPPRLIAP
                     MPTYDLWMAAFRDSEGNIHQLMSEVPRSK"
     gene            9269..>9449
                     /locus_tag="VGR03_02265"
     CDS             9269..>9449
                     /locus_tag="VGR03_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993132.1"
                     /translation="MTDVKPKSFTGRDAAEIADRLHSAAIHLLRRLRVRDRESGVGPA
                     QLSALSVLVFGGPRSL"
BASE COUNT         1931 a         2644 c         2960 g         1914 t
ORIGIN      
        1 gcatcgcgaa atgccgatcg aggcgcagta catcgggcgc aacgtgcaga ccagcgacac
       61 ggagattatt gaagtgcggc tgcgggagat cgacaaggaa gagcgcgtgg tgctggtcga
      121 ccgcgtggag taggtttgaa acttttgttg gaattgcctg gttccacatc cgagaaggga
      181 cattgagtta caacttccgc gatttgctgg ccctcgaacc gctcacggcg gacgaactcg
      241 gctttctcct cgatcaaagc aagccatttc aggaaattca gaagcacccg ctgaagaaac
      301 tggcgaccct gcgcggcaag accgtggcgc tggcgttttt cgaaacttcg acgcgcacgc
      361 gcatcagctt tgggacggcc gcgtcgcggc tcggtgcgga ttccatgaat ttgcaagcag
      421 aggcgtccag cttgaaaaag ggcgagacgc tgctggacac ggttttcaat ctaaacgcga
      481 tgaagccgga ctgcctggtg atgcggcatg cggcatcggg cgcgccgcat tacgtggcgc
      541 ggcatctgga tattcccgtg gtgaacgcgg gcgacggcac gcacgagcat ccttcgcagg
      601 gcttgctgga cgcgctgacg attcgcgacc ggttgggaac gatcaaaaat ttgaacgtga
      661 cgattctcgg cgatatcctg cacagccgcg tggcgcgttc gaacattcat ctgctgggca
      721 aattcggctg ccgcttcacg ctgtgcgggc cggcgatgtg ggtaccgcgt gagctggaga
      781 aaatcgcgcc cggcacggcg attcgctgcg tctaccggat cgaagaggcg ctggaaggcg
      841 cgcacgtcat tatgatgctg cgcgtgcaga ccgagcgact gaatgatccg cctctttccg
      901 ccaacgatta catccttctc tatcaactca acgccgagcg gcttcgccgc gccaacaggg
      961 acgcaatcgt tctgcatcct ggccccatca accgcgggat cgagattacg ccggaggtcg
     1021 ccgatggccc gcagtcctgc gtcctggagc aggtcaccaa cggcgtggca gttcgaatgg
     1081 ctatcctcta tttgttgatc agcggcgcga cggaaacgga cacgcatccg gcgctggaag
     1141 tgaaacccaa ggaatccgcc aatgcttctg attaagaacg ggcgcgtagt cgacccggcg
     1201 tcgaagacgg acgcagctct cgacgtgttg ctggatgggg agaagatcaa agaggtgggg
     1261 ccggccggaa aggttgcagc gccgcagggg acggaaattt ttgacgcaag cggcttgatc
     1321 gtcgcgccgg gatttattga tattcatgtg cacttgcgcg aaccggggca ggaatcttcg
     1381 gaaacgatcg agacggggac gcgggcggcg gcgcgcggcg ggtttacggc agtttgctgc
     1441 atgccgaata cgaagccggt gaacgacaat gcttccgtca cgcggtttat tctcgatcgc
     1501 gcgaaggttg gcgggagcgt gcgggtttgg ccgattggcg cggcgtcggt ggggagcaag
     1561 ggcgaagcga ttgcggaaat cgcggcgatg aaggaagcgg ggatcgtcgg agtttcggat
     1621 gatggaaaac cgatcgcgac ggcgaagctt gcgcggcaag tgatggatta ttgccggtcg
     1681 ctcgatcttc ccgtgatcga gcatgccgaa gatgtttcgc tggctgccgg ggcggtgatg
     1741 cgcgagggag tgacttcgac gcggctgggt ttgcgcggga tgccggcggc ggcggaatcg
     1801 gtttgcgtgg cgcgcgacgt tcagcttgcc gagcttaccg gcgcgcggct acatgtggcg
     1861 catctctcgg caaaggcgtc gctcgaacaa gttcggtggg ccaaatcgcg cggattgcgg
     1921 gtgagctgcg aagtcacgcc gcatcacttc acgctgatcg acgaggacgt gcagtacgat
     1981 tcgaaattca agatgaatcc gccgctggcg gcgcgggaag accgcgaagc attgctggct
     2041 ggattggcgg acggaacggt ggatgcgatt gcgacggatc atgcgccgca tgagcctgcc
     2101 ttgaaagatg tggaattcga tcgcgcaccc ttcggcattc tcggttttga aacggcgctg
     2161 gcgctagcgc tggaacaatt ggtgccgagc ggacggcttt cgctgatgcg cgtggtggag
     2221 cttttcacta cgggtcctgc gcgtgtgctg ggcatcgaac gaaacatcac tgcgggccag
     2281 ccagccgact tgacgatttt ctcaatcgat cgcgcctgga cttacagcgt gaaagaatcg
     2341 ccgagcaaat cgcgcaactc ccccttcgat ggccgcgcct tcaagggggc ggctgtcgcc
     2401 accatcgttg ccgggcgtgt cgttcataag cagtaagaaa cgaaacgtag agacgcagag
     2461 agcgcagaga gcggcactgc gaagtgcaga tggtcgcggc gaattgtcag tgggcggcgg
     2521 gttcggcggc ggggtgcatg ccgtgctggg ggacttcgtg gatgccgcgg gcggggggct
     2581 cgatgcgctg gcggtagaaa tgatcgagcg gcacacggcc tttgccgatg gcgtagccat
     2641 tttcgatggc tttggtgacg taggctttgg cgagcgtggc ggcttcgagt aatccccggc
     2701 ctgcggccaa ttgcgcggtg agcgctgaag caaaggtaca accggtgccg tgcgtgttgt
     2761 ccgtcttcac cctgtcgccg ccgagctgga ccatttcggt gccatcgaac aggatgtcca
     2821 cggcgcgctc catgtggccg cccttgacga tcaccgctcg cgcgcccaat tcgacgagct
     2881 tacgcgcggc ggcttccatg tccgcgacat ccttgatggt cagcccggtg agaacctccg
     2941 cctccggcac attcggcgtg atgacgctcg cgcgcttcag cagttccgct gcgacaaagt
     3001 tgattccgcc cgcatcgagg agttccgcgc cgctggagga cttcatcacg ggatcatgca
     3061 cgacgtgcgc aaatttatgc gcatcgagga attccgccac cacgacggcg ttgccgcggt
     3121 ttcccaacat tccgattttg accgcagcga tctcgcaatc cttggccagc acttcgagct
     3181 gcgcgcgcag ctccgcgctc ggcgtgttgt ggaccgtttc gacgccctgc gtgttctgta
     3241 ccgtcagcga ggtgatcgcg gcgacgccgt agcagccgtg cgcggcaaat gttttcaaat
     3301 cggccgctgt gccggcgccg caggaaggat cgaaacccgc gatggtcagc agaatcggcg
     3361 gcgctgattg actatgtccg ttcgtactca tcgtcttttc ttactcgaag aatgaaaata
     3421 gccggtcgcg tctgcaagac gcaccggcac ttgcgaatgt actcgcgctg tttctcaggt
     3481 gcaagaaaaa acttgatgaa tcgccatatt ctccaaggaa cacttcgctg gtgcgatcgg
     3541 cgtcaaggaa tgaggaagga gtgtgagctg cacgatgcag ccccaagtcg ctatgtgcta
     3601 gagtcacgtc gctgaaacca actcaaggtt gagtgttaca ccctatgaag atcacccgtc
     3661 gatggatcat gaagtgtctg gcaggaatgg cacctgctat gtggaattcg cgcgcgcttg
     3721 gcgctccatg gcaccagcaa ccgggcgctg ctcccgctga gggttcagat cccccagcac
     3781 aatccggtga tgcgacggcg cgtcctaaac gatcgattgc ttcgggacct tttcagccga
     3841 cgtgggattc cctgaagcaa taccagacac ccgattggtt ccgcgatgcc aagttcggaa
     3901 tctgggcgca ttggacagca caatgcgtgc ccgagcaagg cgactggtac gcccggctca
     3961 tgtacatcca ggggctcagg cagaatcagt atcacgtcga gcactacggg cctccatccc
     4021 aattcggatt caaggatatt gacaatctct ggaaagccga caaatgggaa ccggacgcgc
     4081 tcatgcaact ctacgtcaag gccggcgctc gctacttcgt cagcttggcg aaccaccacg
     4141 acaattttga ttgctatgac tcgatgcacc atgaatggaa ctcggtccgc gtcgggccga
     4201 ataaggacat cgtgggaatc tgggcaaaga ccgcccgtaa gcatggacta cgatttgggg
     4261 tgaccaatca ctcggctcac gcttggcact ggtttcagac ggcatacggc tacgaccccg
     4321 agggtcctct cgccggtgtt ccctacgacg ggttcctcaa ccaggaagac gggaaaggga
     4381 agtggtggga gggcttagac ccccagaatc tctacgctgg gcccaacatg gtgatgccga
     4441 agggattcac taccagtaaa gctgcacgag actggcacga gcaacacgat cgcatatgga
     4501 cggaagaccc tccggccaac aaccccgcct tcgtcgagaa gtggtttctc cgctgccagg
     4561 atcttttgga tcgatacagt cccgacctac tttattttga caacacggaa cttcccctgg
     4621 gacaagcagg cttggacatc gccgctcatt tctacaattc caacatgaga aaccaccagg
     4681 accggctgga agcggtgctg aactccaagg aactcaggcc cgatcacgcg ggaactatgg
     4741 tgctcgatat cgagcgtggc cgcgcgaaca aaatcctttc cgctccgtgg cagactgaca
     4801 cctgtatcgg cgactggcac taccggcgct ccctttacga gaaacacgag tacaagaccg
     4861 cggaaaccgt gatcaaggtg cttgttgata tcgtcagcaa gaacggcaat ttacttttga
     4921 acatcccgtt gcgcggcgat ggaagcatcg atgacgacga acgtggggtc cttgctgaac
     4981 tggcaacgtg gatgcccgac aatcaggaag ccattttcgg aactcgtcca ttcgtcgttt
     5041 ttggtgaagg cccgcccgac ctcgcttcca ctggtaattt caatgaaagt aaggggcgcc
     5101 cctatacggc ggaagacatt cgcttcacta ccaagggcga gacgctctat gccatcgcgc
     5161 taggttggcc tgcaaacggc aaaatccaga tcaacacgct tgccagaggt tccgcgaatt
     5221 atccaagaga ggttggaaga gttgaactgc tgggaacaaa aggtgcgctt cccttcaccc
     5281 gcgacgggaa agggctcacg gtgacactgc ctgagaaaaa gcccaatgac tacgcctacg
     5341 ccttgagaat tactctgtga gataatctgt tttctaagtt cctgacctca tgaatgtcaa
     5401 cggcgggtag ccagtcaaaa atccccatgc tttgtgaata gactcgggac gcgcgcccat
     5461 ttagcatcga ctatgggaac aggccacgga cgagggtggc ttcggagacg cgaccgacgg
     5521 caaggatcat ggcgcccgcg cgcgggggca cgtgatgctt gcgcatggtg tccaccattt
     5581 cgtggaaggc gcgagtcatg atcttttgca ggcgcgcgtt gacttcctcg acgtcccaca
     5641 ttaggccttc ctggttctgc acccactcga aatagctgac ggtgacgccg ccggcgttgg
     5701 cgagaatatc cggcagcacg atgaccccgc ggcgcgccag aatttcatca gcgtgcggcg
     5761 tggtgggacc gttggcggct tccgcgacga tgcgggcctt cacggcctcg gcgttgtgga
     5821 gggtaatgac attttcgagc gctgcgggga tgaggatgtc gcacttcatg gttagcagtt
     5881 catcgttggt gatacgcgac gcgccgggca tgcccacaac ggtgccggac cgctccttgt
     5941 agcgaatggc tttgatggga tcgatgccgc gcgtgtttgt cacgccgccg cgcgtatcgc
     6001 tcaacgcgac gatttttgct ttcttttccg cgaacagacg cgccgcggtc gcgccggcgt
     6061 tcccgaatcc ctgaatcgcg acggtcgcac cgcgaagcga aatctttttc agcttgcagg
     6121 cttcctcgac cacgtagagc agcccgcgcg cagtggcttc gaggcgtccc tcggagccgc
     6181 cgatcgaaac cggcttgccg gtgacgactc catgcgccga atggcccacg gtcatggcgt
     6241 aggtgtccat gatccaggcc atggtctgcg cgtcggtgta aacatccggc gcgggaatgt
     6301 cttgggatgg cccgatgatg ggcaggatct cgctggcata gcgccgcgtc atgcgttcga
     6361 gttcgctctt ggacatgcgc ttggggtcgc agatcacgcc gcccttggct ccgccaaacg
     6421 gaacgtttac ggcggcggtc ttccaggtca tccacatcgc cagcgctttc acttcatcca
     6481 gcgtgacgtt gggatggaag cggatgccgc ccttgcctgg gccgcgcgac acattgtgct
     6541 gcacgcggta cccggtgaac actttcacct gaccgttgtc catcttcgtc ggcaaggaaa
     6601 cttccagcac gcgcttggga gtgcgcaaaa tctgccgcag gccgggatcg agcttcaaga
     6661 attcggccgc catgtcaaac tgaatctgcg cgatgcgaaa gggattcagg tcctcgcggg
     6721 gagcaatgcg gggcaccatg ggaggtgcgc ctatctgtct tgtcggggct gtgggctttg
     6781 cgaccgtggc catcggcgtc ggtcctttcg tctactcgat ttgtctaaga aattctgttt
     6841 tcacttggcc caagttcaaa attcgttgga gctgaatccc gtttatccgt ttgccgcaaa
     6901 cttcctggtg ttgatggcgt acgcatcgcg gcaggcgggg gagcagaagt gaagaagctc
     6961 gcctgcttcc cgcaacggaa tggaaagtgt ctcgtccacg tgcataccgc ataccgggtc
     7021 gcgcaccagc cggcgtgctg cagcgctcgt ctgcgccgca ggagaagcat ccgcggtttg
     7081 cggcccggga gcagcctcat tcctgagcat actgccgacg atgcggcgca agagccgcac
     7141 gccccaggaa acgacgagca gccagaacag aaatcgcacg acgcgagcga gaaagttcat
     7201 cttaatgatt gtgtgctcaa cggcccacga ttacaaagac ccacgtggcc gccgtgaggg
     7261 gagtgtatgc aaggcgaaaa gggcccgtca aacgaaaagg tgtaaaatca aggggttgca
     7321 aggcgaattg tgcccttcct ttttggataa aatggggtta agtccaactt aaaccgttta
     7381 ttcacaatga gttacacgaa gtaggcgggg attacagcct gatgtggctc caaaggggca
     7441 aattcatgcg ctcgcgggat cgcgcgcact cacgtcgctg ccggaatccg atcaatgccc
     7501 atgtatgggc gcaagacttc cgggatcagg atcgagccgt ccgcctgctg ataattctcc
     7561 acgacagcga tccaggtgcg gccgacagcg agcccggaac cgttcaaggt gtgaacgaag
     7621 tcactcttgc cgccaccctt gggcctgaag cgaatgccgg cgcggcgggc ttgaaatgct
     7681 tcgcaattcg aacaggaaga gatctcgcgg aattcgttgc ttgcgggcag ccaaacttca
     7741 atgtcgtacg ttttcgagga agcaaagccc atgtcgccgg tacagagcag cacagtacga
     7801 tagtgcaagc cgagtttctg gagaatcgtt tctgcgttgc atacgagggc ttcgtgctct
     7861 tcgtagcttt tctccggatg cgtgaatttg aaaagctcga ccttctggaa ttgatgctgg
     7921 cgaaggatgc cgcgggtgtc tttcccagcg gcgccggctt cggagcggaa gcaggccgtc
     7981 cacgcggtaa cgtaaatcgg aagtttttct tcgggaatgg tttcgtcccg gtacagattg
     8041 tggagctcga cttcagcagt gggcgtgagc caaaagtcgg tgtcctggat tttgaagagg
     8101 tcggcggcga atttcggcaa ctggcccgcg ccggtgaggg acgcggtgtt taccaggaaa
     8161 ggcggcagga tttccgtgta gccgtgctcg cgggtgtgca cgtcaagaaa aaaattggcg
     8221 agggcgcgct cgagacgtgc gccccagcct ttgtaaacgg cgaagcgtgc gccagtgatt
     8281 ttcgtggcgg cggcgagatc gaggatgccg gcgcgctccc caacgtccca atgcggcgtc
     8341 ggcgcgaaat cgaatttcgg cgggttaccc cagcggcgga cttcgaagtt atcggtaaca
     8401 aaatgaccaa aaggcacgct agagtgcgga acgttgggaa tggtgaggag gaggtcgcgc
     8461 tgtttggtgt cgagttcggc gatgcgctcg tcggattgct tgatgcgctc ggagacctgc
     8521 ttcatttcgg cgatcaaggt gccggcgtcc tgcttttcct tcttgaggct ggcgatctct
     8581 tcgctggcct tgttccgctg cgctttgcgc tgctcgttcg cggtgatcaa ctctcgccgc
     8641 tctttatcga gcgcacggta ggcgtccaag tcgagcgcaa tgccgcgctt cttcgccatg
     8701 tccgcaaaga cgtccaagtg gtctctgaaa tagttcaggt catgcatgct cagaagagga
     8761 taccgtaatc cgcacagcgc ttgtagggcg gggttaccgg caacggtcgg aggcgaagag
     8821 ccctatttcg accgcgggac ttcgctcatc agttgatgaa tgtttccctc ggaatcgcgg
     8881 aacgccgcca tccaaagatc gtacgtgggc atcggcgcga tcaggcgcgg cggcgcaaaa
     8941 atttggacac tcaactcgac gagacgctgg taagccgcat tcagatccgg aacccggaag
     9001 tagaggatgg agctgggatg atcgaactcc ggcgtttctg cgcggcccag catcagccgc
     9061 actccggcac agtcaaagaa agccagattg ggcacggtga aaagcagcgg cagccccagc
     9121 acatcacgat agaatgccgt ggcgcgctgc aggtcttgca cattgatgca gatttggccg
     9181 atgttcgtga tgccgaggtt tgccggtgtg ccgctcattg ggaatttacc tccaggaaca
     9241 gatttgttag catagctaac taaagtgtat gacggatgtc aagccaaaaa gtttcacggg
     9301 ccgcgacgcc gccgagatcg ccgaccggct ccactccgca gccatccacc tgctgcggcg
     9361 gctgcgcgtg cgcgaccgcg aaagcggggt tggcccggcg cagctatccg cgctttcggt
     9421 tttggttttc ggcggcccga gatccctgg
//
LOCUS       DASYVO010000070         4046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_226781, whole genome shotgun sequence.
ACCESSION   DASYVO010000070 DASYVO010000000
VERSION     DASYVO010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226781"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VGR03_02270"
     CDS             <1..1086
                     /locus_tag="VGR03_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993133.1"
                     /translation="PQAPSVPAELKPAIWIDPPTNANFLGFGNLSESTAEVAIEAWLY
                     DVRNPSSQAVVGKVYRGAPTDLQVRKFAHQFADEIIGKLSGGLPGVASTQIAFVSSRS
                     GSKEIWEMDYDGSNQRQLTTLKSISLTPRWSPDASRIAFTCFAPSAGLTSAQICMYSL
                     DTGKLVSFPRFRGTNITPAWSPDGTQIIFSSSMQSNPELYVTDISGGHPKRLTNANGA
                     SMSPSWNPKTGQTVAFVSDRGGTPQLYLMNSDGTSATKLDLPEKGYVIDPAWAPNGQL
                     LAFSWRRPNDNYDIYIMDAATRQLVELTRDQGRNERPSWAPDGRHLVFESTRGGTRQI
                     WTMLADGSQPHQLTTGGHNESPNWSTR"
     gene            1219..1971
                     /gene="pal"
                     /locus_tag="VGR03_02275"
     CDS             1219..1971
                     /gene="pal"
                     /locus_tag="VGR03_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEV1993134.1"
                     /translation="MNLASRSLYRAGLLVMLAAAVFTFGCRHPPKAQPRDVSAPPLAA
                     ARPTVTLQASPTSINKGDSSTLSWNSTDATQLTIAPEVGAVTAQGSTKVTPSDSATYT
                     ITATGPGGSASATASVTVNAPPPPVEQPRQPSLDELFLKEVRDAYFDYDKADIRPDAR
                     AALSKTADFLKSYPQLKVNIEGHCDERGSTEYNLGLGDRRASAVKQYIVSLGVPADRI
                     STVSFGKEKPFCTESNESCWTQNRRGHFVKSN"
     gene            2161..3072
                     /gene="ybgF"
                     /locus_tag="VGR03_02280"
     CDS             2161..3072
                     /gene="ybgF"
                     /locus_tag="VGR03_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEV1993135.1"
                     /translation="MRIRHIAIFSAAVLAGALAGSLVGPRPAEAVARELIELQRDVTS
                     LLQGQKDLSTQVTQDHTVMKTLVEQSNDNVGKLASTMSSLQKSVQDVQANSGARLDTM
                     STQVQGLSDNLEEIKSRLGKLNQQLVDLQNTVQSLDAKLSGTAPAPASGTANPTASTG
                     SSPSTSVVPPGAPSADTLYSNGLRDITSGKYDLARSEFQDYLKYYGDTDLASNAQFYL
                     GEIAYSQKNYDQAVGEYEKVLNNYPKSFKLAPARLKKGMALIELGQKPGGVRELREVV
                     KRYPGTEEDRRARAKLKELGVAITPTR"
     gene            3419..>4046
                     /locus_tag="VGR03_02285"
     CDS             3419..>4046
                     /locus_tag="VGR03_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993136.1"
                     /translation="MSRLVLPTLFLIAAICVPSAFGQTTPDLEEGMKPYGGFHGGDID
                     QISLSSGKLVLNIPLVSYPQRGGKINVGFGLRWQNITPRQTEVYYSNTDTYVEHAFRD
                     ASSVSVGQLDFVDNLGWSAIAGSAYSPDQSLHQMMTPDLGATYRSIDATGLLYSTANT
                     TLQTSNGLRYIYSSPYKVEDPNGNFVTSTSTGWIDTLGRTITSPGHATL"
BASE COUNT          825 a         1434 c          978 g          809 t
ORIGIN      
        1 cctcaggccc ccagcgtccc cgccgagctg aagcctgcaa tctggatcga tccccccaca
       61 aacgccaatt ttctcggctt cggcaatctc tccgaatcca ccgccgaagt ggccatagaa
      121 gcatggctct atgacgtgcg caatccctct tcccaagcgg tcgtcggaaa ggtgtatcgc
      181 ggcgctccca cggatcttca agtccgcaaa ttcgcccatc aatttgccga cgaaatcatt
      241 ggcaagctct ccggcggtct ccccggcgtg gcctcaacgc agatcgcctt cgtcagctcg
      301 cgcagcggtt ccaaagaaat ctgggagatg gactacgacg gttccaacca gcgccagctc
      361 actactctga aatcgatttc cctcacgccc cggtggtctc ccgatgcttc gcgcatcgct
      421 ttcacctgtt tcgcgccgtc cgcgggcctt accagcgcgc agatttgcat gtattcgctg
      481 gacaccggca aacttgtgtc cttcccgcgc ttccgcggta ccaacatcac tcccgcttgg
      541 tctcccgacg gcacgcagat tattttttca tcttcgatgc agagcaatcc tgagctttac
      601 gtcaccgaca tcagcggcgg ccaccccaag cgcttgacta acgccaacgg cgcaagtatg
      661 tcccccagtt ggaacccgaa aaccgggcag accgtcgcct tcgtcagcga tcgcggcggc
      721 actccccagc tctacctgat gaattccgat ggcaccagcg caaccaaact ggacctcccc
      781 gaaaaaggct acgtcatcga tccggcgtgg gccccgaacg gacagcttct ggctttcagt
      841 tggcgccgcc ccaacgacaa ttacgatatc tacatcatgg acgccgccac ccgccagctt
      901 gtggagttaa cgcgcgatca gggccgcaat gagcgcccca gctgggcgcc ggacggccgc
      961 cacctcgttt tcgaatcgac ccgcggcggc acgcgccaga tctggactat gctggccgat
     1021 ggctcccagc cgcaccaact tacgaccggc ggccacaatg aatcgcccaa ctggtcgaca
     1081 cgctgatttt cttggaccta agtttgtgta agatgaataa tatatcgtga tacgagatat
     1141 tacccgaacg cagcccgctg gtgttacgat agtgcttctg gtgggacgaa cacgggaatt
     1201 tcactgagga ggatttccat gaacctggct tcacgctcgc tctatcgcgc tggcttgctc
     1261 gtgatgcttg ccgctgctgt ctttaccttt ggctgcaggc acccgccgaa ggcgcagcca
     1321 cgagatgtgt cagcgccgcc gctcgcggcc gcgcgtccca ccgtgacgct gcaggctagc
     1381 ccaacttcca tcaacaaagg tgactcctca accctgagct ggaattccac cgatgccacg
     1441 cagctcacca tcgcgcccga agtcggcgcc gtgactgctc agggctcgac caaggtcacg
     1501 ccgtccgact ccgcgaccta cacgatcact gccacgggtc ccggcggctc cgccagcgct
     1561 accgccagcg tcacggtgaa tgctccgcct ccgcctgtgg agcaacctcg tcaaccctct
     1621 ctcgatgagc tcttcctcaa ggaagtgcgc gatgcctatt ttgactacga caaggccgac
     1681 attcgtcccg atgcccgcgc ggcgctctcg aagaccgccg acttcctgaa gagctatccg
     1741 cagctcaaag tgaacatcga aggccactgc gacgaacgtg gctccacgga atacaacttg
     1801 ggcctgggcg atcgccgcgc cagcgccgtg aagcagtaca tcgtttctct gggcgttccc
     1861 gccgaccgca tcagcaccgt tagcttcggc aaggagaagc ccttctgcac cgagagcaac
     1921 gagtcctgct ggacccagaa ccgccgcggc catttcgtga agtccaactg agccgcttca
     1981 ccctcttcgc caccatgacc gattgctgac gggatcagcc gcgaggctgg caaatttttg
     2041 ccacctcgcg gcggcactta ttagagtgaa agcggttccc ccggcctgta tgattttgtt
     2101 ggctgctctg cagccatgat tcgttttcag cccttcggag ccccttagga ggattgttcc
     2161 atgcgtattc gccatatcgc cattttcagc gccgccgttc tggccggcgc acttgccgga
     2221 agtctcgtcg gcccacgccc cgccgaagca gtcgcccgcg aactcatcga gctccagcgc
     2281 gacgtcacct ccctgctcca gggccagaag gatctctcca cccaggtgac ccaggaccac
     2341 accgtcatga agacgctcgt cgagcaatcc aacgataacg ttggcaagct cgcctccacc
     2401 atgagctcgc tccagaaatc cgtccaggat gtgcaggcca attccggcgc gcgcctcgac
     2461 accatgtcca cgcaggtcca gggtctctcg gacaacctcg aggaaatcaa gtcgcgcctc
     2521 ggcaagctca accagcagct cgtcgatctt cagaataccg tccaaagcct cgacgccaaa
     2581 ctttccggca ccgcgcccgc cccggcgtcc ggtaccgcca acccgacggc ttccaccggt
     2641 tcctcgccca gcacctccgt cgttcccccc ggcgcgccct ccgccgacac gctctattcc
     2701 aacggcctgc gcgacatcac cagcggcaag tacgatctcg ctcgctccga attccaggac
     2761 tacctcaagt attacggcga caccgacctc gcttccaacg cccagttcta tctcggtgaa
     2821 atcgcttact cccagaaaaa ctacgatcaa gccgtcggtg aatacgaaaa agttttgaac
     2881 aattatccca agagcttcaa gctcgccccc gcgcgcctca agaaaggcat ggccctcatc
     2941 gagctcggcc agaaacccgg cggcgtccgc gaactccgcg aagtcgtcaa gcgctatccc
     3001 ggcaccgaag aagaccgccg cgcccgcgcc aaactcaaag aactaggcgt cgccatcacc
     3061 cccacccgct aactccactc ttcctcgtcg tcattccgtc cccctcgttt gtcatcccga
     3121 gcgcagcgag ggacctgctt ttgcttcgcc ccgcgtcttc gtagcgccag catccctgct
     3181 ggcgattccc tcccctcccc cgcccgtcat ttcgaacgaa tgtgagaaat ccctcctctc
     3241 tccacttccc ccgacgtgcc gacactccgc tcgccacttc cccaaatagt cgttgacaca
     3301 ctccttatca atcttatata aactccacat cgtttcccgc tcggacggca atcgctgttc
     3361 cggtccccgc cgcatgctcg atcgccccgt ggacccgcaa cccggaggtt ccatgcgagt
     3421 gtctcgtctc gttctcccca cgctgttcct tattgccgca atatgcgttc cgtctgcatt
     3481 cggacaaaca actcctgatc tcgaagaggg catgaagccc tacgggggtt ttcatggtgg
     3541 cgatatcgac cagatctctc tcagtagcgg caaactcgtt ctcaatatcc ctctcgtttc
     3601 gtaccctcag cgtggcggca aaataaacgt gggctttggc ctccgctggc agaatatcac
     3661 accacgtcaa acagaagttt actattcgaa tactgatacc tacgtggaac acgcattccg
     3721 cgacgccagt agcgtttccg tcggccagct cgatttcgtc gacaatctgg gctggtccgc
     3781 catcgctggg tcggcttatt cacccgacca atccttgcat cagatgatga ccccagactt
     3841 aggtgctacg tacagatcca tcgatgctac tggacttctc tacagtacag ccaacacaac
     3901 acttcagaca tctaacgggc tccgatacat atactccagc ccatataaag tcgaagaccc
     3961 gaacggaaat ttcgtaactt ccactagcac ggggtggatc gacaccctcg gcaggaccat
     4021 cacgtcgccg ggccatgcaa ccttaa
//
LOCUS       DASYVO010000071         1869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_29518, whole genome shotgun sequence.
ACCESSION   DASYVO010000071 DASYVO010000000
VERSION     DASYVO010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29518"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VGR03_02290"
     CDS             complement(<1..941)
                     /locus_tag="VGR03_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1993137.1"
                     /translation="MATANNSRRTFLKTAGAGLAGTALGSRASSYASIIGANDRVRVG
                     VVGFSDRLRYALVPAFQLHAKELNFELAALSDIWKQRREEGVTHLQHVTGTTIAAARN
                     NEELYERKDVDAVIVATADFQHAQHGVQAVTAGRDAYIEKPLANTMADARAIREAVRS
                     SGKIVQIGTQRRSAANYQRAYEFIRSGQFGDIVSVEMTWNVNQPGRWRRPKLVASLRE
                     EDVDWKRFLVNRPSVPFDPRKYAEFRLFWPYSSGIPDQWMVHQIDTVHWFTGLPRPRS
                     VVANGGIYLWHDGRKNWDTMTAVFDYGPLDDSSKGFQV"
     gene            complement(967..>1869)
                     /locus_tag="VGR03_02295"
     CDS             complement(967..>1869)
                     /locus_tag="VGR03_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEV1993138.1"
                     /translation="SSGASPLPAMSSRFKLSVITDEITQDLGHALEIASKEFGLAYIE
                     LRSLWNKNIINLDEKETAEIRRLLGKYSLQVTDIASPLFKTDWPGAPTSKYSPKTPQF
                     GADYNFAQQGEVLERSIAIAKALGTDRVRCFDFWRLEDPKPFRDSMDGKLRDTAAQVA
                     SKKITLLLENEFACNTATGADSVRTLQAVKSPNFKLNWDPGNAAYRGEIAFPDGFANI
                     PKERIGHMHCKDVVRKADGTFEWAAMGRGIIDYVGQFRALVQAGYSGTMSLETHWKGA
                     GSEEESSRQSMAGMKDQLKKAGAL"
BASE COUNT          349 a          542 c          567 g          411 t
ORIGIN      
        1 acttggaagc ctttggagga atcatccagc gggccgtagt caaacacggc ggtcatggtg
       61 tcccagtttt tccggccgtc gtgccacaga taaatccctc cgttcgcgac gacgctccgc
      121 ggacgcggca ggccagtgaa ccaatgcacc gtgtcgatct gatggaccat ccattgatcg
      181 ggaatgccgg aggaatacgg ccagaacagg cggaattcag cgtacttgcg cggatcaaac
      241 ggcacggaag gacgattgac caggaatcgt ttccaatcaa cgtcttcctc gcggagagag
      301 gcaacgagct ttggacgccg ccagcggccg ggctggttca cgttccaggt catttccacg
      361 ctgacgatgt caccaaattg gcccgagcgg atgaattcgt aagcgcgctg gtagttcgcc
      421 gcgctgcggc gttgcgttcc gatttgcacg attttgccgg aactccgaac ggcttctcga
      481 atggcgcgcg catcggccat ggtattggcg agcggttttt cgatgtacgc gtcgcggccg
      541 gccgttacgg cctgcacgcc atgctgcgcg tgttggaagt ccgcggtggc gacaatcacg
      601 gcgtccacat ctttgcgttc gtagagttcc tcattgtttc gcgccgccgc gatggtcgtg
      661 ccggtgacat gctgcaagtg agtgacgcct tcctcgcggc gctgcttcca aatgtccgag
      721 agggcggcca gctcgaaatt caattccttg gcgtgcaatt gaaaagcagg aaccagggca
      781 taccgcaacc ggtcggagaa cccgacaacg cccactcgta cacggtcgtt tgcgccgatg
      841 atgctggcgt agctggaggc gcggctgcca agagcggtgc ccgccaagcc tgctcccgcc
      901 gttttcagaa aagtacgccg cgaattgttt gcggtggcca tgtttgcctc caccgaagaa
      961 gcgcgatcaa agcgcccctg ccttcttcag ttgatccttc attcccgcca tgctttggcg
     1021 cgaagattct tcctcggagc ccgcgccttt ccagtgcgtt tcgaggctca tggttccgga
     1081 gtatccggcc tgcaccagcg cgcggaactg gccgacgtaa tcgatgatgc cccggcccat
     1141 ggccgcccat tcgaacgtgc cgtccgcttt gcgaaccaca tctttacaat gcatgtgccc
     1201 gatgcgttcc ttggggatat ttgcgaaccc gtccggaaag gcgatctccc cgcgataggc
     1261 ggcattcccg ggatcccagt tcaatttgaa gtttggcgac tttacggcct ggagggtacg
     1321 cacggagtcg gcgccggtcg cggtgttgca ggcgaattcg ttttcgagaa gaagagtgat
     1381 cttcttcgag gcgacttgag cagcggtgtc gcggagtttg ccgtccatcg agtcgcggaa
     1441 gggcttcgga tcctccaacc tccagaaatc gaagcagcgg acgcggtcgg tgcccagggc
     1501 cttggcgatc gcgatggaac gctccagcac ctctccttgt tgcgcgaaat tgtagtccgc
     1561 gccgaactga ggtgtcttcg ggctgtactt ggaggtgggg gctccgggcc agtccgtttt
     1621 gaagagcggg ctggcaatgt cggttacctg aaggctatat tttcccagta gccgccgtat
     1681 ttcggcggtt tccttttcat cgaggttaat gatgttcttg ttccacaagc ttcgcagctc
     1741 gatgtaagcc aggccgaact ctttggaagc aatttccagg gcgtgaccga gatcctgcgt
     1801 gatctcatcg gtgatgaccg aaagcttgaa gcggctactc atcgccggca aaggcgatgc
     1861 gccagaact
//
LOCUS       DASYVO010000072         3771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_226839, whole genome shotgun sequence.
ACCESSION   DASYVO010000072 DASYVO010000000
VERSION     DASYVO010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226839"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VGR03_02300"
     CDS             complement(<1..822)
                     /locus_tag="VGR03_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1993139.1"
                     /translation="MTVARAAREFLRHSVLARVGFAAAVILLLTALFAPWIAPADPAA
                     QNLPSRLASPSRAHWMGTDELGRDILSRTLFGARISLLVSVCVVCGCGSIGLTMGMIA
                     GYAGGWLDRFVNLLLINAFLSFPGILLAIAFAAFLGPGIGKVILALIITGWAGYARLA
                     RAQVLKVKELEFILAARSLGASHVRIMARHLLPNILQPVLIQATIGMAGAILAESTLS
                     FLGLGVLAPVPSWGAMLNDARGHLFDAPHMVVFPALAVMTAVLSFNLLGDAWRDWL"
     gene            complement(819..1739)
                     /locus_tag="VGR03_02305"
     CDS             complement(819..1739)
                     /locus_tag="VGR03_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1993140.1"
                     /translation="MARYFLKRFLLTLPALWLVLTLVFLMIHIVPGDPVEQMLGEGAA
                     PGQLTQLRHALGLDLPLHTQYGHYFWQLGRGDLGQSFKFQAPVRQIIFERYPATLQLA
                     FLALIVCAAIAIPAGMLAAYRRGQSADRAVGLFTLFGLAFPNFALGPVLILLFSIELG
                     LLPVSGRGRPLYYVLPAATLGAALAAILTRMIRGSMLEELSSDYVRTARAKGLSTSAV
                     LLRHAFRNALIPIITILGLQFGTLLAGTIVTETIFSWPGIGRLTVQAISARDYPLLQG
                     CILVIAVSYVAVNLLTDLLYAVIDPRVSLS"
     gene            complement(1754..3163)
                     /locus_tag="VGR03_02310"
     CDS             complement(1754..3163)
                     /locus_tag="VGR03_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEV1993141.1"
                     /translation="MLAVRLIGVLALVALNGFFAATEFSLVAVRLSRVRQLVAKGNAR
                     ARVVEVLLGDLHRVVSGVQLGITLSSLAIGALGEATFAKIFQAIWPGALGAHTALFAH
                     AIALACAFALLSALHVVIGELVPKTISLARAERVALLVALPFQWFLNTFHWAINMLDG
                     VSGAIVKALGVTSPQVHGVAHSTEELQIQIQQARERGLIAPGEEKFILSAMELAQIQV
                     REIMVPRPDIHTLPVESNLDEVMRVFATTQRSRIPVYRGNADHILGFVHIKDMIWILL
                     DRERRLEENLSPPPFDLRRVLREALIVPETKPANELLLELRTRHVGMALVVDEFGSIL
                     GLVTIEDILEQMVGEIHDEFDVVEQPLTLADGALIFDAALNVRDLDTQYNITLPEDPA
                     YATVGGFVLDQLGFIPKGGENFEYANFRFSVVEMDGKRVARVKIQRIRPAENESNTQV
                     FSEAVLQPPRDSKATRENP"
     gene            3308..>3771
                     /locus_tag="VGR03_02315"
     CDS             3308..>3771
                     /locus_tag="VGR03_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEV1993142.1"
                     /translation="MRTILRIFYWVFAILVIVTASVSWWFIYRPLPQIDGSAALPGLQ
                     REVTVERDGWGVPHIRAATVEDLAEAQGYVMAQDRLWQMDLLRRAARGQLSEILGART
                     LKIDESFRTYGFGRAAERDAALLDAESRKVMEAYARGVNQFIEQHKKNLPLE"
BASE COUNT          889 a         1073 c         1128 g          681 t
ORIGIN      
        1 gagccagtcg cgccacgcat caccgagcag gttgaaggag agcaccgcgg tcatcaccgc
       61 caacgcgggg aatacaacca tgtgcggcgc atcgaagaga tggccacgcg catcgttgag
      121 catggcaccc caactcggaa cgggtgcgag cacaccgagc cccagaaaac tcaaggtaga
      181 ttccgcgagg atggctccgg ccatgccgat ggtggcctga attagaactg gttgaagaat
      241 gttcggaagc aagtgcctcg ccataatgcg cacgtgcgag gcgcccagcg accgcgccgc
      301 gagaatgaat tccagctctt ttacttttag cacttgcgcg cgggccaggc gtgcgtagcc
      361 cgcccatccg gtgataatca gggccagaat cacttttcca atgcctgggc cgagaaacgc
      421 tgcaaacgca atcgcaagca gaattcctgg aaacgacaga aaagcgttga tcagtagaag
      481 attgacgaag cgatcgagcc aacctccggc gtatcctgca atcattccca tcgtcaagcc
      541 gatggatcca caaccgcata cgacgcagac ggaaacgagc agcgaaatcc gcgcgccaaa
      601 taaagtgcgc gacaaaatgt cccggccgag ttcgtccgtt cccatccagt gcgcgcgcga
      661 gggagatgcg agcctcgagg gcaggttctg cgcggcaggg tcggctggtg caatccaggg
      721 cgcaaacagt gcagtcaata acagaatgac ggctgccgca aatccaacgc gcgccaaaac
      781 actgtgacgt agaaattccc gggctgcccg cgccacggtc atgacaagct cacccgggga
      841 tcgatcacag cgtaaagcaa gtccgtgagc aggttcacgg cgacgtaaga gaccgcaatg
      901 accaggatgc atccttgcaa caagggatag tctcgcgcgg agatggcttg aacggtcaat
      961 cgtccgatgc ctggccacga aaagatggtc tcggtcacga tcgtgccagc gaggagcgtt
     1021 ccgaattgca gtcccaaaat ggtgatgatg ggaatcaacg cgttgcgaaa agcatgccgc
     1081 agcaggacag cgctggtgga gagccctttg gctctggcgg tgcgcacgta atcgctcgaa
     1141 agctcttcga gcatggagcc gcgaatcatg cgagtgagaa tcgcggccag cgcggctccg
     1201 agcgtcgcgg caggcagcac gtaatagagt ggtctaccgc gccctgaaac cggcaagaga
     1261 ccaagttcaa tggaaaagag aagaatcaag acaggtccca gcgcgaaatt ggggaaagcc
     1321 agtccaaaca gagtgaagag acccacggcg cggtccgccg attggccacg tcgataggcg
     1381 gcaagcatcc cagcgggaat ggcgatcgcg gcgcagacaa tcagcgcgag gaaagcaagt
     1441 tggagcgttg cggggtaacg ttcaaagata atctggcgca cgggcgcttg aaacttgaag
     1501 gattgcccaa gatcgccgcg gccgagttgc caaaagtagt gtccgtactg ggtatggaga
     1561 ggcaaatcca acccaagtgc atgacgcaat tgagtcagtt gtcccggtgc tgcgccctcg
     1621 cccagcatct gctcgacggg atcgcccggc acgatatgaa tcatgagaaa gacgagcgtg
     1681 agaacgagcc agagggccgg cagcgtgagg agaaatcgtt tgaggaagta gcgcgccatt
     1741 tgctgtgaat aacctaagga ttctcccgtg tggctttcga atctctgggc ggttgcagca
     1801 cggcttccga aaacacctgc gtgttgcttt cattttcagc cggacgaatg cgttgaatct
     1861 tcactcgtgc aacgcgcttt ccgtccattt ccacgaccga gaagcgaaag ttggcatact
     1921 caaagttttc tccacccttg ggaatgaaac ccaattggtc gaggacgaaa ccacctacgg
     1981 tggcgtaagc cgggtcctcc ggcaaggtga tgttgtattg cgtgtcgagg tcgcgcacat
     2041 tcagggccgc gtcaaaaatt aacgcgccat ccgcgagtgt aaggggctgc tcgacaacgt
     2101 caaattcgtc gtgaatctct cccaccatct gttcgaggat gtcttctatg gtcaccagcc
     2161 cgaggatgct gccaaattcg tccacgacca gggccatgcc gacgtgacgg gtgcgcaact
     2221 cgagaagcaa ttcgttggcg ggcttagtct cgggcacgat gagcgcttcg cgcaagacgc
     2281 ggcgcaaatc gaaagggggc ggcgataggt tttcctcgag ccgacgttcg cgatcgagga
     2341 ggatccagat catgtctttg atatgaacaa agccgaggat gtggtccgcg ttgccgcgat
     2401 agaccgggat tcgcgaacgc tgggttgtcg cgaaaacccg catcacctcg tccaagttgg
     2461 attccacggg aagcgtgtgg atgtccgggc gcggcaccat aatctcccgc acctggatct
     2521 gagcaagttc catggcgctg agaatgaact tttcttcgcc gggcgcaatc agtccgcgct
     2581 cgcgggcttg ttggatttgg atctggagct cctcggtcga atgggcaacg ccgtgtacct
     2641 gcggcgaagt cacacctagg gccttcacga ttgcgcccga aacgccgtca agcatgttga
     2701 tcgcccaatg aaacgtgttc agaaaccatt ggaacggtag tgcgaccagc agcgcaacgc
     2761 gctccgcgcg ggcaaggctg attgtcttcg ggactaactc cccaatcacg acgtgcagcg
     2821 cactcaaaag ggcaaacgcg caggcgagtg cgatggcatg ggcaaagagc gcggtgtgcg
     2881 cccccaaagc gccgggccaa atggcctgaa atatcttcgc aaaagtggct tcgccaagcg
     2941 cgccgatcgc caggcttgag agcgtgatgc ctagctgcac gcccgaaacg acgcggtgca
     3001 aatcaccgag gagtacttca accacgcgcg cccgcgcatt cccctttgcc accagctgcc
     3061 ggacgcgtga caatcgtacg gcgacgaggg aaaactccgt cgccgcaaaa aaaccattca
     3121 gcgcgaccaa cgccaagaca ccaatcaggc gcactgccaa cataggcgga tactttctcc
     3181 ctgcagcatt gtaacagctt ggtgttcgcc agacgtgagc ccgttcggac tgatcactaa
     3241 cactcggttg cggaccggat gcacctcctc tacactcaat ttttgcctaa atcgggtggg
     3301 agtgaacttg cgaaccatac ttcggatctt ctactgggtc ttcgccattc tggtgattgt
     3361 gaccgcctcc gtgtcctggt ggtttattta ccgcccgctg ccgcagatcg atgggagcgc
     3421 tgccctaccc ggactccagc gggaagtcac tgtagagcgc gatggctggg gcgtgccgca
     3481 cattcgcgct gcaacggtag aagaccttgc ggaggcgcag ggctacgtca tggcgcagga
     3541 ccgcctctgg cagatggatc tgctccgccg cgccgcccgc gggcagcttt ctgaaatcct
     3601 gggagccagg acgctcaaga ttgatgagag ctttcgaacg tacggattcg gacgcgctgc
     3661 tgaacgagat gccgcgttgc tcgatgccga atctcgcaag gtgatggaag cctatgcgcg
     3721 cggcgtgaac caatttatcg agcagcacaa aaagaatctc cctctggaat t
//
LOCUS       DASYVO010000073        10681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_212621, whole genome shotgun sequence.
ACCESSION   DASYVO010000073 DASYVO010000000
VERSION     DASYVO010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 10681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10681
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212621"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VGR03_02320"
     CDS             <1..592
                     /locus_tag="VGR03_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993143.1"
                     /translation="SVEFEAIKREVLALPVVKKPAPGAAGAVVTPAPPSRSFAEYQAS
                     SYTLKYPDNWKKYPDSNGGGVSFAPEGGVVDDGSGHSALAYGLIVGLVQSKGDPNDGN
                     ALNTATSQVIQDLQQSNPSMKITRQGERLRLNGQPGFSTYLSNVSPAGGQETDWVVTV
                     LRPQGLVYFVCVAPQSSYDNYDKTFSSILDSVRFTR"
     gene            complement(927..2438)
                     /gene="gcvPB"
                     /locus_tag="VGR03_02325"
     CDS             complement(927..2438)
                     /gene="gcvPB"
                     /locus_tag="VGR03_02325"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEV1993144.1"
                     /translation="MNDRIKKASRHTNQSEGLIFEKSSPGKRAFELPPLDVPAADLAK
                     ALGANHRRGGIEGFPEVSEIEVLRHFTRLSTWNYAIDLGMYPLGSCTMKYNPRVNEFV
                     ARLDGIATEHPYQPQELSQGCLKILRLLEKCLLEITGMDAITLQPAAGAHGEMTGLLL
                     IRAYLQKQGNPRKKVLIPDSAHGTNPATAVIAGYEVENIKSESHGQIDVDKLRDLVTG
                     DVAALMITNPSTLGIFEQRIPEIAEILHAKGALLYMDGANMNALTGVTRPGDLGVDVM
                     HLNLHKTFSTPHGGGGPGAGPVAMKKILEPFRPVPILVEKEGGRLALEPVRPDSIGRV
                     KAFSGNFGVLVRALAYILAYGPGVRQATEDAVLNANYIRKQLEGVYELPYSLPSMHEV
                     VFSDAIQKKNGVSTMDIAKRLIDYGFHPYTTAFPLIVAGAMMIEPTESESKEECDLFI
                     DAMKSIAEEAATRPEIVKTAPHTTRVRRLDEVGAARKPVLRWKPVSPRSEATD"
     gene            complement(2505..3851)
                     /gene="gcvPA"
                     /locus_tag="VGR03_02330"
     CDS             complement(2505..3851)
                     /gene="gcvPA"
                     /locus_tag="VGR03_02330"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEV1993145.1"
                     /translation="MRYLPKSPAERQEMLAAIGARSIDELFSSIPERFRLREALKIPG
                     PYSEAEVIQYFKDRAAENSNGYTSFLGAGVYNHLRSVVTDAIIQRGEFLTSYTPYQAE
                     ITQGTLQAIFEFQTLMCQLTGQEVANASMYDGSTAMTEAVLMAERVTGRQLVLVARSV
                     HPEYREVLKTYAKNSGLRVEEIPFTASGTVDAKALQAALKDDVGAVVVQSPNFFGVIE
                     SYAPLADATHAAGAMFVVAIAEGVSLGLLKPPAEADIVAMEGQSFGLPPSYGGPFAGV
                     IASRDKFVRQMPGRLAGQTTDSEGQRGFVLTLATREQHIRREKATSNICTNQALCALA
                     ATVHLTLLGKEGLREMAEQNLSKAQFALSELLKIPGVRRTFDAPFFNEFTVELPRSVK
                     IVNGQLLREKIIGPYALGTPYPELTKRALVCVTETTTRAEIERFAGALKSALLRPI"
     gene            complement(3851..4252)
                     /gene="gcvH"
                     /locus_tag="VGR03_02335"
     CDS             complement(3851..4252)
                     /gene="gcvH"
                     /locus_tag="VGR03_02335"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEV1993146.1"
                     /translation="MAYPKTYRYTKEHEWISEEGGLATIGITDYAQHELGDVVFVELP
                     KPGAKIEVGKSFGTVESVKAVSEIYAPAAGEVIEANGELQNTPEKINTDPHGVAWLIK
                     VRLTNAAELSGLMDAAAYEAYIAAKGQEASA"
     gene            complement(4284..5462)
                     /gene="gcvT"
                     /locus_tag="VGR03_02340"
     CDS             complement(4284..5462)
                     /gene="gcvT"
                     /locus_tag="VGR03_02340"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEV1993147.1"
                     /translation="MESAATPHKSELRKTPLNALHRRLGAKMVNFGGWDMPVEYPSSG
                     GLIAEHKAVRASVGVFDVSHMGDIRIRSGSKPGGALAAVQHISMNDASKLAIGQAHYS
                     AMLYPEGTFVDDVIVHRLGEDDYLLVINAGTREKDINWVRENTKSFGCVVEELSDAYT
                     QLAIQGPRAVDVMKKLTDAELNGIKNYWFTHGTVCGLNNTLIARTGYTGEDGFEIYVP
                     SDVETSEKVWNQVMEAGREFAVVPCGLGCRNTLRLESKMALYGHEISDKINVWEAGLD
                     RYCKMEKGDFVGRAALGQARAAGLRRTLVGIEMVDRGIARDEYRCCNEAGDAIGVVTS
                     GSPSPSLGKNIALAYVPPSYSAVGTTLYVEIRGNKCKAQVVPTPFYKRPKKATSPRES
                     "
     gene            6027..6206
                     /locus_tag="VGR03_02345"
     CDS             6027..6206
                     /locus_tag="VGR03_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993148.1"
                     /translation="MQAPTDCAQCDLPEDRCTCERYCTICKGQVNVRLCTDGLYYCPD
                     CREACDVALATSRGH"
     gene            6196..6624
                     /locus_tag="VGR03_02350"
     CDS             6196..6624
                     /locus_tag="VGR03_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993149.1"
                     /translation="MATEPKTFRLLLDADARLAAAAGGVVRHLADVAGLESDAIARLQ
                     SAVIAACSEAFEHLTADHPRLEITFTRLSDRIEVALSHRGEGFPAVGLDTIAGFAAQV
                     GGTESNPGVFAVFDRVQYETHGGEALTRLTKYIGQVAPSI"
     gene            complement(6702..7760)
                     /locus_tag="VGR03_02355"
     CDS             complement(6702..7760)
                     /locus_tag="VGR03_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993150.1"
                     /translation="MKLTTNSGVTRTRPGSTIRRLVAAGILLAMSLAMPKPARAGRLG
                     ADIIALFPKEIGEFAYADLKKARTLKWFPQLQEQMLPDRFRQFEKFLASAGVDPNSQV
                     DELAWGLVAEGMSAKTEGTGSTDVPTGEEIVGVALGTYNPNSTEAFFKQQKLPSVKAR
                     GYTLYAFGTGSGSNDLFFFFLDSNTAAFGHRAVLEKMIEVRFGSEEGLLRNDKLFPLI
                     NEANGSGVVWAVLNPAYTRLAMQQLAPEVGQFPEAAKLVARMQNMIINVDASSGIDGK
                     FQAVCGSTEDANTLGQLLQAGFLYKRYQAQKENPDLAQLLDQARVTPAGDRVMIRMSL
                     SDDQMASLIRKNTFALKM"
     gene            complement(7800..9338)
                     /gene="ftcD"
                     /locus_tag="VGR03_02360"
     CDS             complement(7800..9338)
                     /gene="ftcD"
                     /locus_tag="VGR03_02360"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEV1993151.1"
                     /translation="MKRLIECVPNFSEGRDPAKVDAIVAAMSGVPGVYVLDRKMDADH
                     NRCVITLAGEPDAVGEAALLGTGKAMELIDLTKHSGAHPRVGATDVLPFIPIDGVTIE
                     DCVALARRVGNEIWKRYRIPVFFYEAAATRPDRVNLENVRRGQFEGLREEMKNNHERQ
                     PDVGEPKLHPTAGVTVVGARKFLVAYNVNLNTSDISIANKIAKAIRFSSGGLRYVKSM
                     GVELKARKLAQVSINLTDFEQTPMHRVYEMVKREAERYGVMPVGSEIVGLVPKKAIEM
                     AADFFLQLENFSPAQVFENKLAAALSGAPLEATKDGRLAGLARPFLDAVASPAATPGG
                     GSVSAFAGALAAALGQMVAGLSRKKKSQAGFADQLSAALDELRRIVDELAEAIDRDAA
                     SYDAVIAAFKLSQGDAQETRQREEAIQKATKGASEVPLQVAERTVALFERLGQLDAIA
                     AASMRSDLQVARLMAEAGARGALANVEINLDGLTDAAYAASMRMKIAALRERLGNAPR
                     ATTA"
     gene            complement(9335..9805)
                     /locus_tag="VGR03_02365"
     CDS             complement(9335..9805)
                     /locus_tag="VGR03_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993152.1"
                     /translation="MRLLKIVPQKTKSLFEEYFYGFLVTICVVVMILGKAGGLSGYDA
                     IVTGLWVGGFTWFAWQVRRYRKEQANPGRLEKSKQAVQAPPQMTKKPLPPNMKPMIGP
                     QWPLKTGQVPPQNAQATGPQGKKPAFVYERPTLPDRKPKLPHNWPGQQNKKPKQ"
     gene            complement(9831..>10681)
                     /gene="hutI"
                     /locus_tag="VGR03_02370"
     CDS             complement(9831..>10681)
                     /gene="hutI"
                     /locus_tag="VGR03_02370"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEV1993153.1"
                     /translation="TTTVEAKSGYGLDVTSELKILRLHKELAVEQPLEIVSTFLGAHV
                     VPAEYRGKTGGAERYIQLMEQNLLPEIGENRLAEFCDVFCERGAFTVEQSKRVLQAGR
                     QWGLAPRLHAEQLSRTGAARLAILMRAASCDHLEKVNRADIAALGKSETVATLLPGCD
                     FHLGLKQYAPARALIEAGAIVSLATDYNPGTSPTLSMPMILSLACTQLRMTPAEAIAA
                     ATINAAYALRREKSIGSLEVGKQADLAVFEVADYREIPYYFGVNHCWMTVKRGRVIHA
                     AEQAPI"
BASE COUNT         1985 a         3428 c         2920 g         2348 t
ORIGIN      
        1 ttccgttgaa ttcgaagcca tcaagcgcga agtcctggcc ctgccggtag tgaaaaaacc
       61 ggcccccgga gcggctggag ccgtcgtgac tcctgcgccg ccatcacgga gcttcgcgga
      121 gtatcaagcg agttcgtaca cgctcaagta tccggacaat tggaagaagt atcccgacag
      181 caacggaggc ggcgtttcct ttgctcccga aggcggggtc gtggatgacg gcagcggcca
      241 tagcgccctc gcgtatgggc tgatcgtcgg cttggtgcag tccaagggcg atcccaacga
      301 tgggaacgct ctgaatactg cgacgagcca ggtgattcag gatcttcaac agtcgaatcc
      361 cagcatgaaa atcacgcggc aaggcgagcg tttgcgcttg aacggccagc cgggcttttc
      421 cacctatcta agcaatgttt ctccggccgg cggccaggaa acggattggg tcgtcaccgt
      481 cctgcgtccg cagggtttgg tctatttcgt ctgcgtcgcc ccgcaatcct cctacgacaa
      541 ttacgacaag acgttttcgt cgattctcga ctcggtgcgg ttcacaagat agaaagaaaa
      601 atcaaggcgg agggcgcagt acagactcgc ttgcggcgac actccggaag caatcgcaaa
      661 cgccatcgct agcgcgaaga ggcctacggt cacgccgacg atcagatcgg aaaggaattt
      721 gtggcgttta tagctgcgca gcaggagtat cgatttcggg agccaggctg cgagggcgga
      781 tgtcgcgcgg cttccggtcc agaaaagaaa atgtctatgc agattctcgc aaggagcaaa
      841 acgagatggt cacgcgacta agtcgatgtg tgcgttgaag ggagtcttgg gctgacgcgt
      901 tccggttcga cggttttccc gttctcttag tccgttgctt cgctgcgcgg ggagaccggc
      961 ttccagcgca aaaccggttt gcgcgcagcg ccgacttcat cgagacggcg gacgcgcgtc
     1021 gtgtgcggcg cggtcttcac aatttccggc ctcgtcgcgg cttcctctgc gatggacttc
     1081 atcgcatcga tgaagaggtc gcattcttcc ttggactcgg attccgtcgg ctcgatcatc
     1141 attgctcccg ccacaatcag cggaaacgcc gtcgtgtacg gatgaaagcc gtagtcgatg
     1201 agccgcttcg caatgtccat ggtgctcacg ccgtttttct tttgaatggc gtcgctgaac
     1261 accacttcgt gcatcgaggg cagggaatag ggcagttcgt acacgccctc aagctgcttt
     1321 cgaatgtaat tcgcgttcag caccgcgtct tccgtggcct gccgcactcc cgggccgtag
     1381 gccaggatgt acgccagtgc gcgaaccagc acgccaaagt ttcctgagaa tgctttcacc
     1441 ctgccgatcg aatccggccg cactggttcc agcgccagcc gcccgccttc tttttccacc
     1501 aggatgggca cggggcggaa gggctcgagg attttcttca tcgccaccgg acctgcgccg
     1561 gggccgccgc cgccgtgcgg cgtcgaaaac gttttgtgca ggttgaggtg catcacgtct
     1621 acgcccagat cgcccggccg tgtcacgccc gtgagcgcgt tcatgttcgc gccgtccatg
     1681 tagagcagcg cgcccttggc gtgcaggatt tccgcgatct ccggaatccg ctgttcgaaa
     1741 attcccagcg tcgatggatt ggtgatcatc agcgccgcaa catctccggt caccaagtcg
     1801 cgcagcttat caacatcaat ctggccgtgc gattcggact tgatgttttc gacttcgtat
     1861 cccgcgatca ccgccgttgc cggattcgtc ccgtgcgcgg aatccggaat caaaaccttt
     1921 ttgcgtggat tcccctgctt ctgcagatac gcgcggatca gcagcaggcc ggtcatttct
     1981 ccatgcgctc ccgcagcggg ctggagcgtg atcgcatcca tgccggtgat ttcgagcagg
     2041 cacttttcga gcagccgcag aatcttcaga cagccttgcg aaagttcctg cggctgataa
     2101 ggatgctcgg tggcaattcc atcgagtcgg gcaacgaact cattgacgcg cgggttgtac
     2161 ttcatcgtgc aggagcccag cggatacatg cccagatcga tggcatagtt ccaagtcgaa
     2221 agacgcgtga agtggcggag aacttcgatc tcgctcactt cgggaaaacc ttcgatgccc
     2281 ccgcgccggt gattcgcacc gagagctttg gcgagatcgg ccgcgggcac gtcgagcggc
     2341 ggcaattcaa acgcgcgctt tcccggcgac gacttttcaa agatgagccc ttcgctctga
     2401 ttcgtgtgtc tcgaagcctt tttgattctg tcgttcattc tcagctttct actttcaggg
     2461 gcgcccgccg cggcggattg cgccattgcg agtaatgcgt tccttcaaat gggccgcagg
     2521 agcgcgctct tcagcgcgcc cgcgaatcgt tcgatttccg cgcgcgttgt cgtttccgtg
     2581 acgcacacca gcgcgcgctt ggtcagttcc ggatacggcg tgcccagcgc gtacggcccg
     2641 atgatttttt cgcgtaaaag ctggccattg actatcttta ccgagcgcgg cagctcgacc
     2701 gtgaattcgt tgaagaacgg cgcatcgaat gtccgccgca ccccgggaat cttcagtaac
     2761 tccgaaagcg caaactgcgc cttcgataag ttctgctccg ccatctcgcg caagccttcc
     2821 ttgccgagca gcgtcaggtg caccgtagcg gccagcgcgc agagcgcctg gttcgtgcaa
     2881 atgtttgaag tagccttctc gcggcgaata tgctgttcgc gcgtcgcgag cgtgagcacg
     2941 aatccgcgct ggccctcgga atccgtcgtt tgtcccgcga gccgcccggg catttgccgc
     3001 acgaatttgt cccgcgaggc aatcacgccg gcgaacggcc cgccgtaact cggcggcaat
     3061 ccgaaactct ggccttccat cgcgacgatg tccgcttccg ccggcggctt gagaaggccg
     3121 agggaaacac cttccgcaat cgcgactaca aacatcgcac cagccgcgtg cgtcgcatct
     3181 gcgagcggcg cgtaggactc gatcacgcca aaaaaattcg gagactgcac gaccaccgcc
     3241 ccgacgtcat ccttcaatgc agcctgtagc gccttggcgt cgaccgtgcc gctggccgtg
     3301 aagggaatct cctccacgcg caatcccgaa ttcttcgcgt aagtcttcag cacctcgcgg
     3361 tactccggat gcacggatcg cgccactagc acgagctgcc tgcccgtcac gcgctccgcc
     3421 atcaacaccg cctcggtcat cgccgtcgat ccgtcgtaca tcgaggcgtt cgccacttcc
     3481 tgcccggtta gctggcacat cagcgtttga aactcgaaga tcgcctgcag cgtgccctgg
     3541 gtgatttccg cctgataggg cgtgtaggag gtgaggaact ctccgcgctg gatgatcgcg
     3601 tccgttacca cggagcgcag atggttgtag acgcccgctc caagaaagct cgtgtacccg
     3661 tttgaatttt ccgcggctcg gtccttgaaa tactgaatga cctcggcttc ggagtacggt
     3721 ccaggaatct tcagtgcttc gcgcagtcga aagcgttcgg gaatgctgga aaacagttcg
     3781 tcgatggatc ttgcgccgat ggcggcgagc atctcttgcc gctcggcggg gctcttcggg
     3841 aggtagcgca tcaggccgag gcttcctgac ctttggccgc gatgtaggct tcgtacgccg
     3901 ccgcgtccat cagcccgctg agctcagcgg cattcgtcag gcgcaccttg atcagccacg
     3961 ctacgccgtg cggatcggta ttgattttct cgggcgtatt ctgaagttcg ccgttggctt
     4021 cgatcacttc gcccgcggcg ggcgcataaa tctcgcttac ggccttgacc gattccaccg
     4081 tgccaaacga cttcccgact tcgattttcg ctccgggttt gggcagttcg acgaagacca
     4141 cgtcgcccag ttcatgttgt gcgtagtcgg taatcccgat cgttgcaagt ccgccttcct
     4201 cgctgatcca ttcgtgttct ttggtgtaac ggtacgtctt cggataggcc atcagctcac
     4261 tccttggatt cgttcgcgaa cgatcaggat tcgcgcggcg acgttgcttt cttcggccgc
     4321 ttgtaaaacg gtgtcgggac cacctgcgcc ttgcacttgt tcccacgaat ctccacgtac
     4381 aaagtcgtgc ccaccgccga atacgacggc ggcacataag ccagcgcaat gttttttccc
     4441 agcgacggtg aaggcgaccc gctcgtcacc accccgatgg catcgcccgc ttcattgcag
     4501 cagcgatatt cgtcgcgcgc aattccgcgg tcgaccatct caatccccac gagcgtgcgc
     4561 cgcaatcccg ccgctctcgc ctgccccagc gccgcgcgcc ccacgaaatc gcctttctcc
     4621 atcttgcagt agcgatccag cccggcttcc cacacattga tcttgtcgct gatctcgtgt
     4681 ccgtacagcg ccatcttcga ttccagccgc agcgtattcc ggcatcccag gccgcaggga
     4741 acgactgcga attcgcgccc cgcttccatc acctggttcc agaccttctc gctggtctcc
     4801 acatcggagg gcacgtaaat ctcaaaacca tcctcgccgg tgtatccggt gcgcgcgatc
     4861 aaggtgttat tcaggccgca gaccgttcca tgcgtgaacc agtaattctt gatgccgttc
     4921 aattccgcgt ccgtcagctt cttcatcaca tcgacagctc gcggtccctg aatcgccaat
     4981 tgcgtgtagg cgtcgcttaa ctcctcgacg acgcaaccga aactcttggt attctcgcgc
     5041 acccagttga tgtctttctc gcgcgtcccg gcattgatca ccagcagata atcatcttcg
     5101 cccagccggt gcacgatcac gtcatccaca aacgtgcctt ccggatagag catcgcggaa
     5161 tagtgcgctt ggccgatggc cagcttcgac gcatcgttca tgctgatgtg ctgaaccgcc
     5221 gccagcgccc ccccgggctt gctgccgctg cggatgcgaa tgtcacccat gtggctgacg
     5281 tcgaaaacgc ccacgctggc gcgaaccgcc ttgtgttccg caatcagccc gccgctcgat
     5341 gggtattcga caggcatgtc ccagccaccg aaattgacca tctttgcgcc aaggcggcgg
     5401 tgcaatgcat tcagcggcgt cttacgaagc tcgcttttgt gaggagttgc ggcggattcc
     5461 attccaatgg ctcaagcaga aactgatact agcacgcgct ctccaacggg tcaaaggcgc
     5521 cctcccggcg gtagtgggat gttttgaatt tggaggaatt ggtgggatta ttagcgcaga
     5581 aggcgcaggg agcgggagcg tgccactccg aaagcgtgtt ttcatatcgc taatagttag
     5641 catcgcgacg gcggttttat tgctggcagt aggccgagcg accaattcaa aggtgctttt
     5701 cctcccgcag ttgccgggat tctttgcatt cctcagcata tggggagttc actctggcga
     5761 aggaaactct gttgcagggc ttatcgtcgt cagcgcggca aatgcgctgg tgtattggcc
     5821 agtcgtactc ggattgagct ttttactccg aaacagaggg tcaaactgaa gatgtttagt
     5881 atgtaaaact catacaggat tcaaaacagt ccactaccct cccggccacc ctccccccta
     5941 acggaggtga gtgactcgcg catcactctc accttcgtct tgccgcagcc tcccatgctc
     6001 aggtagagtt ttccggagag gaccttatgc aagctccgac cgattgcgcg caatgcgact
     6061 tgccggaaga tcgttgcacc tgcgaaaggt actgcaccat ctgcaagggc caggtaaacg
     6121 ttcgcctttg caccgacggt ctctactatt gcccggattg ccgggaagcc tgcgacgtcg
     6181 cactggccac cagccgtggc cactgaaccc aaaacctttc gcctgctact cgatgccgac
     6241 gcccgcctgg cggccgcggc cggcggcgtg gtccgccatc tcgccgatgt cgcgggactc
     6301 gagagcgacg ccatcgctcg attgcaatcc gccgtcatcg ctgcttgctc ggaagctttc
     6361 gaacatctaa cggcagatca tccgcggctg gaaatcacct tcacgcgctt atccgatcgc
     6421 atagaagtcg cgctatccca tagaggcgaa ggctttccag cggttggcct cgatacgatt
     6481 gcaggatttg ccgcgcaagt tggcggcacg gaatccaacc ccggcgtttt cgccgttttc
     6541 gatcgcgtcc agtacgaaac gcatggcgga gaagccctca ctcgcctgac caagtacatc
     6601 ggccaggtcg ctcccagcat ttaacagtgg ttcgaaacga aaaaaaccag acagcgcggc
     6661 tgtctggccc ttttctcttg cgcggtattg ttttcagagc cttacatctt cagcgcgaac
     6721 gtgttttttc gaataagcga tgccatctgg tcgtcgctca gcgacatccg aatcattact
     6781 cgatcgcccg cgggcgtcac gcgcgcctga tcgagcagtt gcgccagatc cggattttcc
     6841 ttctgcgctt gataccgctt atagaggaac cccgcctgca gcaattggcc cagcgtgttc
     6901 gcatcttcgg tcgatccgca caccgcctgg aattttccgt cgatgccgct gctggcgtcc
     6961 acgttgataa tcatgttctg catccgcgcg acgagtttcg ctgcttcggg gaactgtccc
     7021 acttccggcg ccagttgctg catcgccaaa cgtgtatagg cgggattcag caccgcccag
     7081 accacgccgc tgccgttcgc ttcgttgatc agcgggaaga gtttatcgtt gcgcagcaaa
     7141 ccttcctcac tgccaaagcg cacctcgatc atcttttcga gaacggcgcg atggccaaac
     7201 gccgcggtat tcgaatcgag gaaaaagaag aacagatcgt ttgatcccga acccgtgccg
     7261 aaggcataaa gcgtgtaacc acgcgctttg acgcttggaa gcttttgctg cttgaagaac
     7321 gcctccgtcg aattcggatt gtaagtcccc agtgccacgc cgacgatctc ttctccggtg
     7381 ggaacgtccg tcgagccggt gccttcggtc ttcgcgctca tcccttccgc gaccagcccc
     7441 caggcgagtt catcgacttg cgaattcgga tcaacacccg ccgaagccag gaatttttca
     7501 aattgccgga agcggtcagg gagcatctgt tcctgcaatt gcggaaacca tttcagcgtc
     7561 cgagcctttt tcaggtcggc gtaggcaaat tccccgatct ccttggggaa gagcgcgatg
     7621 atgtccgcgc caaggcggcc cgcccgcgcg ggcttcggca tcgccaggga catcgccaac
     7681 aaaatgcccg ccgccacgag tctgcgaatc gttgaccccg gccgggtccg cgtcacacct
     7741 gaattcgttg tcagtttcac gtcgtgctcc tttcgaatgg ggcagaggga actcttagtc
     7801 tacgccgttg tcgcgcgggg tgcattgccc agacgctccc gcagtgctgc gatcttcatg
     7861 cgcattgagg cggcatacgc cgcgtccgtc aggccgtcga gattgatctc gacgttcgcc
     7921 agtgctccgc gagcgcctgc ctcagccatc agccgtgcta cttgcaagtc tgaccgcatg
     7981 gatgcggcgg cgatagcatc cagttgtccg aggcgttcga aaagcgcaac ggtacgctcg
     8041 gccacctgca acggcacttc cgacgcgcct ttcgtggcct tctgtatggc ttcctcgcgc
     8101 tgccgcgttt cctgcgcgtc cccttgcgac aatttaaacg cggcaatcac cgcgtcgtag
     8161 gacgccgcgt cacgatcgat ggcctcggca agttcatcta caattctccg cagttcatcc
     8221 agcgcggccg agagctgatc ggcaaatccg gcttgcgatt tcttttttcg cgaaagcccc
     8281 gcaaccatct gccccagtgc cgccgccagt gctcccgcaa acgccgagac gcttcctcct
     8341 cccggcgtgg ccgcggggga tgccacggcg tccagaaacg ggcgcgccaa tcccgccagc
     8401 ctcccatcct tcgtcgcttc cagcggcgcg ccgctcagcg cggccgccag cttattttca
     8461 aacacctgag caggagagaa gttctccaac tgtaggaaaa agtccgccgc catctcgatc
     8521 gcttttttcg gcaccagccc gacgatctca ctccccaccg gcatcacgcc gtagcgctcc
     8581 gcttcgcgct tcaccatctc atacacgcga tgcatcggcg tttgttcgaa gtccgtcagg
     8641 ttgatggaca cctgcgccag cttgcgcgct ttcagttcca cgcccatcga cttcacatac
     8701 cgcagtccgc cagatgaaaa ccggatcgct ttcgcgattt tgttcgcgat gctgatatcg
     8761 gaagtattga gattcacgtt gtaggccacc agaaacttgc gcgcgccgac gacggtcacg
     8821 cccgccgtgg ggtgtagttt tggttcccca acgtccggct ggcgctcatg attattcttc
     8881 atttcctcgc gcagcccttc aaactgccct cgccgcacat tttctagatt tacgcggtcc
     8941 ggccgtgtcg ccgccgcttc gtaaaaaaac accggtatcc ggtagcgctt ccagatttcg
     9001 ttgcccactc gccgggccag cgccacgcaa tcctcgatgg tcacgccatc gatgggaata
     9061 aacggcagca cgtccgttgc gcccacgcgc ggatgtgccc cgctgtgttt cgtcaggtcg
     9121 atcagttcca tggccttgcc ggttcccagc agagccgcct ctcccaccgc gtccggttcg
     9181 cccgccagcg tgatgacgca acggttatgg tcggcatcca tctttcgatc cagcacgtac
     9241 acacccggca cgccgctcat cgccgcaacg atggcatcga ccttcgcggg gtcgcgccct
     9301 tcggaaaaat tcggcacgca ctctatcagt cgtttcattg ttttggtttt ttgttttgtt
     9361 gtccgggcca attgtgcggc agttttggct tccggtccgg caacgtcggt ctttcataca
     9421 caaacgctgg tttctttccc tgcggccccg tggcctgcgc attctgcggt ggcacctgtc
     9481 ccgttttcag cggccattgc ggcccgatca tcggcttcat gtttggaggc agcggcttct
     9541 tcgtcatttg cggcggcgct tggaccgcct gcttggactt ctccaggcgg ccaggattcg
     9601 cttgctcttt ccggtagcgt ctcacctgcc aagcgaacca cgtgaagcct cccacccaca
     9661 gccccgtaac gatggcatcg taaccgctaa ggccccctgc ttttcccagg atcataacaa
     9721 ccacgcagat agttaccaag aagccgtaga aatattcttc gaatagactc ttcgtcttct
     9781 gcggcacgat cttgagaagg cgcaccttcc gtaccggcct cttcgaatag ttagattggg
     9841 gcttgttcgg cggcatggat tacgcgacca cgcttcacgg tcatccagca atggtttaca
     9901 ccaaaataat agggaatttc ccggtagtcg gccacctcga atacggcgag gtccgcttgt
     9961 ttgccaacct ccagcgatcc gatgcttttt tcccgccgca acgcataagc cgcattaatc
    10021 gtcgccgccg caatcgcctc ggccggagtc atgcgcagtt gcgtgcacgc cagcgagaga
    10081 atcatcggca tgctcagagt cggactcgtt cccggattgt agtccgtcgc cagtgacacg
    10141 atcgctcccg cctcaatcaa agcccgcgcc ggcgcatatt gcttcagccc cagatgaaag
    10201 tcgcatcccg gcagcagcgt cgccacggtt tccgattttc ccagcgccgc gatatcggcg
    10261 cgattcactt tctccagatg atcgcagctt gctgcccgca tcaggatcgc cagccgcgcc
    10321 gcgcccgtgc gcgaaagctg ctcggcgtgc aaccgcggag ccagccccca ttgccggccc
    10381 gcttgcagca cgcgttttga ttgctctacc gtgaacgccc cgcgctcgca aaacacatcg
    10441 caaaactccg ccagccggtt ttctccgatc tccggcagca gattttgctc catcaactgg
    10501 atgtagcgct ccgctccgcc cgtttttccc cgatactccg ccggtacgac gtgcgcgcca
    10561 agaaatgtgg acacgatctc caacggctgt tccaccgcca gctctttgtg caatcgcaga
    10621 atcttcaatt cgctcgtaac gtccagcccg tacccgctct tagcctcgac cgtcgtcgtg
    10681 c
//
LOCUS       DASYVO010000074         8793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_157826, whole genome shotgun sequence.
ACCESSION   DASYVO010000074 DASYVO010000000
VERSION     DASYVO010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8793
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157826"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2089)
                     /locus_tag="VGR03_02375"
     CDS             complement(<1..2089)
                     /locus_tag="VGR03_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV1993154.1"
                     /translation="MRFDGIHGTTYDSAHRSYLMVGKTFAHYTVLEKAGAGGMGVVFR
                     ARDETLHRDVALKLPSTNALTDAESRELILREARAASALNHPHICTIYEVGEVDGQPY
                     IAMEYLAGETLSRRIPPNGLPTESVLDLGAQIADAMDHAHTQGILHRDLKSANVRMTS
                     SGQLKVLDFGLAMNIKEASFEGVTRSTSLDSGSVVGTLAYMAPELFQAKAPDVRTDLW
                     SLGVLLYELAAGALPFQGRTGFELTTAILRESPAALPTHVTPGLRAVIMHCLSKEPEK
                     RYQRASEVRAALEALQSDTGVAPRAALQPERKSATWYVLAAVSLLAAVAIVLFLYGRI
                     SKPTAGPQTGGKLRLFISSEGNLSGPTLSPDGKMVAYIQDNDGRQELYVSRVAGGEHV
                     KLTNDDSQKEDPQFSPDGERIAFVRRVANATQAELCAIPALGGGIVPLVSGAEHPAWS
                     PDGSRLAFILRRQGEPEALATSAADGSDTRVILKSDDVYPFFGRLSWSLDGTVLAISR
                     SRGGMNREIWTVPAHGGVGTHLTNDVAGVVSDSPIFTPDGRGVIHRSNRGGASNLWWQ
                     PLDAKPPVQLTTGPGPDSSPSVARDGTIAFLNSRSRHSILVHSLADGSSTTLRTDASR
                     LWAPAFSPDGKEIAYSRDEPDGSWHLWIIPASGGAVRQVTSGKVPEIYPRYTPDGSSI
                     YFNTWGTEPLSLWR"
     gene            2216..3454
                     /locus_tag="VGR03_02380"
     CDS             2216..3454
                     /locus_tag="VGR03_02380"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV1993155.1"
                     /translation="MSSNGNALGNGKNGKNGKKRRRIIWGAIAVVVLGGAGYGVKAAL
                     SPSRTIDPSKIVSAERGDLARVVVATGKIQPLSKVEIKSKASGIVKKLYVDYGETVKQ
                     GQLLAELDKVQLEAAERASRANLQAAEAALDSSKATLERNKVDAEGPDVPFLKLNMGR
                     AEQMFQDGVMSKSLVEDAEKNYQLALNKQVSAQRNLAVSRAEIAKSEAQVAQARASLE
                     NAQEDLRNSTITSPIEGLVLSRDVNVGDAVSSILVLGSQATLIMTLGDVSEVYVQGKV
                     DEADIGKVFFSQPARIVVESFKDKKFTGKVTKISPLGKEKDNVTTFEVRVSISNPGGE
                     LKANMSANAEILLEEKKNVLMVPEAALIYDKERKASVEIPDAKADNGKKKLSVKLGIS
                     NGVKTEILEGLGEKQQVILQ"
     gene            3477..4733
                     /locus_tag="VGR03_02385"
     CDS             3477..4733
                     /locus_tag="VGR03_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1993156.1"
                     /translation="MSFQDLLRDTLATLWAHKRRTMLTMFGIAWGIISITVMVAAGEG
                     LGKGIQNNQETFGKDVMIVFAGRTSMQAGGTRSGRIIRWSEEDYVQVAKEAPACKYVM
                     PELGNNVQVHSLFNSGDLQVVGALPPFTEIRSIAVAQGRFYNEEDNAEARNVAFLGSD
                     AKKQLFADREALGQTISLNGVPYTVIGVMKPKEQNSSYDGADVRKVFIPFNSMRRDFP
                     NKPPAIEHSVDRLLVAPWTLETHLDCVRQVRRTLGRLHSFDPRDKEAASIWDTVKNAE
                     ANRMIIVGMEVFMGAVGVATLFLGGLGVMNVMLVSVRERTREIGVRKALGATRESILR
                     QFFLETIIVVALSGGTGLLLSYGFCALVNLLPMPLFFAGLLTSWKLGALSIFLLGLIA
                     VLSALYPANRAASVDPIEALRFEAGG"
     gene            complement(4824..5201)
                     /locus_tag="VGR03_02390"
     CDS             complement(4824..5201)
                     /locus_tag="VGR03_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEV1993157.1"
                     /translation="MQDYRQLKVWQTSHSLVLRIYSATATFPEPERYGLTSQMRRAAA
                     SVPANIAEGCGRAGPAELRQFLYISVGSATELEYFLLLARDLRFLTEKQQEALDTSVR
                     EIKRMLTGLIQRVEQGPTATRPG"
     gene            5281..6534
                     /locus_tag="VGR03_02395"
     CDS             5281..6534
                     /locus_tag="VGR03_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1993158.1"
                     /translation="MLTEIFKEAWAALGRNPVRSLLTMTGIAWGIVAVTLLLSYGSGF
                     RNVLMYTFEVFGKGAVVCWPGTTSEQAGGERAGKAVRFEQEDADWVKAQSPLVKRVTR
                     ETVRWKGITHDARLSDTAIRGVYPEYGEMRNEIPMEGRWISPEDIAERRRVAFLGAIL
                     RRKLFSGTPAIGETVRIDGVKFTVVGSMDTKFSDSNYFTSDDESVFIPYTTAGDLWDA
                     RYASVMLFEPVAPNFEADAMQQFRAAIANRQHFSPNDKRAITMFGREEFKPIYEGITL
                     GIEALLFFVGALTLGIGGVGVMNIMLVSVDERVREIGLRRALGAKKSHIRWQFLLEAL
                     VLTLAAGVIGMLLSAAITSAIGTLPFLGPAYEDDTGKVDIKLTLSFVTMMLSTAILVV
                     VGVISGWLPAMQAAKLDPVEALRYE"
     gene            complement(6682..7224)
                     /locus_tag="VGR03_02400"
     CDS             complement(6682..7224)
                     /locus_tag="VGR03_02400"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV1993159.1"
                     /translation="MRILRKIAAWGVLAAALGVILLFAMPSYRQGEASIAGTTAKDFP
                     LDIAGKPGHLTDLKGKVVILNFWATWCPPCVEETPALNRLQKYIEARNGVILGVAADE
                     DPTAYEKFLREQGVVFPTYRDPLTRDNHSPIAQSYGTSMYPETYVIDRHGKIARKFIG
                     FQQWDSPNMLAYFDAILGLT"
     gene            7287..8360
                     /locus_tag="VGR03_02405"
     CDS             7287..8360
                     /locus_tag="VGR03_02405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1993160.1"
                     /translation="MSSEAHNPCTSGTLVSATTAGSVTAIVPARNEEAVIAACIASLA
                     NQPEIAEILVVNDQSTDGTASVVRSLTEKIPNLRLLESTGLPDGWVGKNHALWVGVQQ
                     AKCAWLLFTDADAEHERDSVSRALRIAQKQGAVLVSFSPEQVTETWYEKALIPFIYLR
                     LAKRFSYEKVNDPNSPAAAANGQFLMMRRDVYDAIGGHSSVAGEVLEDVALAMRVKAA
                     GQRISFGSGKGIVRVRMYRSFPEMWQGWKKNLYRLMGGTPWAVVREMESNLPWIPVLL
                     ILAGLKYPFLLFLGVLLLIARQTSYGLDLARNQYPFSFIFYYVPAVALYLAVLWASYR
                     SHVNGRIQWKGREYSVGAPESAR"
     gene            8371..>8793
                     /locus_tag="VGR03_02410"
     CDS             8371..>8793
                     /locus_tag="VGR03_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEV1993161.1"
                     /translation="MVLLTRKIEFAASHLYHNPNFSVEENRRIFGKCNNPHGHGHNYT
                     LEVTVEGEPDPVTGMVLDLKELKEILEREVMQRMDHRHLNYEVAELAGQIPTCENIAR
                     VIWQLLEPKITQGRLHRVRLYESPDLFADCYRNGARAAG"
BASE COUNT         1853 a         2649 c         2525 g         1766 t
ORIGIN      
        1 cgcgccagag actcaatggt tccgttcccc aggtattgaa gtaaatcgag ctgccgtcag
       61 gcgtgtagcg cggatagatc tccggcactt ttccagaagt aacctgccgt accgctcctc
      121 cactcgccgg aatgatccac agatgccacg atccgtccgg ttcatcgcgt gaataggcga
      181 tctccttgcc atcgggcgaa aacgccggag cccacaagcg cgaagcgtcc gtccggagcg
      241 tcgtgctcga gccatccgcc agtgaatgga cgagaattga gtgtcgcgag cgggaattca
      301 gaaacgcaat cgtgccatcg cgcgccacac tgggggaact atccgggccg gggccggtcg
      361 tcaattggac tggcggcttg gcgtccagcg gctgccacca gaggttcgag gcgccaccgc
      421 gattggaacg gtgaatgaca ccgcggccat ccggcgtgaa gatcggcgaa tcgctgacca
      481 cgcctgcgac gtcgttcgtg aggtgcgtcc ccacccctcc atgcgcaggc acggtccata
      541 tttcccgatt cataccgccg cggcttcgcg aaatggccag aaccgtgcca tcaagcgacc
      601 aagatagacg cccaaaaaaa ggatagacat catcgctttt cagaatgacg cgcgtgtccg
      661 agccgtcggc agcggatgtc gccaaagctt ccggctctcc ttgcctccgc aggatgaacg
      721 ccagcctgct gccgtcggga gaccacgcgg ggtgttcggc tccggaaacc aggggcacga
      781 taccgccgcc cagcgctggt attgcgcaca gctccgcctg cgtcgcattg gcaacgcggc
      841 gcacaaaggc gatcctctcg ccatctgggg agaattgcgg atcttccttc tgggaatcat
      901 cattcgttaa cttcacatgc tcgccgcctg ccacgcggct gacgtaaagc tcttgtcggc
      961 cgtcattgtc ctgaatgtag gcgaccatct tgccgtccgg ggacagggtt ggcccactaa
     1021 ggttgccctc cgaagaaatg aagagccgca acttaccgcc cgtttgtgga ccagctgtgg
     1081 gcttgctaat gcggccgtaa aggaacagaa caatggccac cgcggccagg agactcactg
     1141 ccgccagcac ataccaagtc gcggattttc tttccggctg caaggctgcc cgtggtgcga
     1201 ccccggtgtc ggactgcagc gcctctagag ccgcgcgaac ctcactggct cgctgataac
     1261 gtttctccgg ctcttttgac aggcagtgca tgattacggc acggagtccc ggcgtcacgt
     1321 gcgtcggtaa tgccgccggt gattcccgca gaatagccgt cgtcaattcg aatcccgtgc
     1381 gcccttgaaa gggaagcgcc cccgcggcga gttcgtagag aagcactcct aaggaccaga
     1441 ggtcggtgcg cacatcgggg gccttcgcct ggaacaactc cggcgccatg taggccagcg
     1501 ttccgacaac gctccccgaa tcgagactcg tcgaacgtgt gacgccttcg aacgatgcct
     1561 ccttaatatt catcgccagg ccgaagtcca gcaccttcag ctggcccgac gaagtcattc
     1621 gaacgtttgc cgatttcaaa tcccgatgga gaattccctg cgtatgagcg tggtccatgg
     1681 cgtcggcaat ttgcgcgcca agatccagca cggattctgt gggcagtcca ttgggtggga
     1741 tgcgacggct cagcgtttcg ccggcgaggt attccatggc gatgtacggc tggccatcga
     1801 cctcgcccac ttcataaatc gtgcagatgt gcggatgatt cagggcggag gcggcacgcg
     1861 cttcgcgcag aatgagctcg cgcgactccg catccgtgag cgcgttcgtc gagggcagct
     1921 tgagggccac gtcgcggtgg agcgtttcgt cccgggcacg aaaaaccacg cccataccgc
     1981 cggcgccagc tttttccagc acggtgtagt gtgcgaatgt cttgccaacc atcaggtagg
     2041 acctatgcgc ggaatcatag gtggtcccgt gaatcccgtc aaacctcatg ggcgcctggc
     2101 gcggggaccc gaccaaaagc ctcgcccatc gtctaaacaa gcaggacgag ggaacttttc
     2161 ccacaccgtt gcgtattccc ttgtagaagc gaggccaaga cagcgggggt ccggtatgtc
     2221 cagtaatggc aatgctttag gaaacgggaa aaacggcaaa aacggcaaga agcgccgccg
     2281 catcatctgg ggcgcaatcg cagtggtggt gttggggggc gccggttatg gcgtcaaggc
     2341 ggcgctcagc ccaagccgca ccattgaccc ttccaagatt gtttccgcgg agcgcggtga
     2401 cttggcgcgc gtggtcgtgg ccaccggcaa gattcagccg ctttccaaag tggagatcaa
     2461 atccaaggcc agtggcatcg tgaaaaagct ttacgtggat tacggcgaga ctgtgaagca
     2521 gggccagttg ctcgcggaac tcgacaaagt gcaactcgaa gccgccgagc gcgcctcccg
     2581 tgccaatttg caagccgccg aagcggcgct ggattcatcc aaggccacac tagaacgcaa
     2641 caaagtcgat gcggaaggtc ctgacgtgcc gtttctgaag ctcaatatgg ggcgcgccga
     2701 gcagatgttc caagacggcg tgatgtccaa gagcctcgtc gaagacgcgg agaaaaacta
     2761 tcagctcgcg ctgaacaagc aagtcagcgc gcagcgcaat ctcgccgtct ccagggccga
     2821 aattgccaaa tccgaagctc aagtggcaca ggcgagggct tcgctggaaa acgcgcagga
     2881 agatctgcgc aactccacca tcaccagccc gattgagggc ctggtcctct ctcgcgatgt
     2941 aaatgttggc gacgcggtca gttctattct agtgctcggc tcgcaggcta ccttgatcat
     3001 gacgcttggt gacgtcagcg aagtttacgt gcaaggcaag gtcgatgaag cggacatcgg
     3061 caaggttttt ttcagtcagc ccgcgcgcat cgtcgtggag tccttcaagg acaagaaatt
     3121 caccggcaag gtcaccaaga tttccccgct cggcaaggag aaggacaacg tcaccacctt
     3181 cgaagtgcgc gtctcgattt ccaatcccgg cggtgaactc aaggccaaca tgagcgccaa
     3241 cgccgaaatt ctcctcgagg aaaagaaaaa cgtgctgatg gttccggaag ccgcgctgat
     3301 ttacgacaaa gaacgaaaag cctccgtaga gatccccgac gccaaggccg acaacggcaa
     3361 gaaaaaactc agcgtaaagc tcggcatctc gaacggcgtg aagacggaga tcctcgaggg
     3421 cctcggcgag aagcaacaag tcattctgca atagttcaat cgacccgcgg agcgccatgt
     3481 cattccagga cttactgcga gacacgctgg ccacgctctg ggcccataaa cgccgaacca
     3541 tgctcacgat gttcggcatc gcctggggca tcatctccat taccgtgatg gtcgcggcgg
     3601 gcgaggggct tggcaagggc attcagaata accaggaaac ttttggcaaa gacgtcatga
     3661 tcgtttttgc cggccgcact agcatgcagg ctggtgggac acgttccggc cgcattatcc
     3721 gttggagcga ggaagactat gttcaggtcg cgaaggaggc gccagcctgc aaatacgtga
     3781 tgccggagct tggtaacaac gtccaggtcc atagcctctt caacagcggc gatcttcagg
     3841 ttgttggcgc gctgcctcca ttcactgaaa ttcgcagcat tgccgtcgca caggggcgct
     3901 tctacaacga agaggacaac gccgaggctc gcaacgtcgc ctttctcggc agcgacgcga
     3961 aaaagcagct ctttgccgac cgcgaagccc tcggccagac gatctcgttg aacggggttc
     4021 cgtatacggt tattggagtc atgaagccga aagaacagaa ttccagctac gatggcgcgg
     4081 atgtgcgcaa ggttttcatc ccattcaatt cgatgcgccg cgatttcccg aacaagccgc
     4141 ctgccatcga gcacagcgtg gatcgtttgc tcgtcgcgcc ctggacgctg gagactcatc
     4201 ttgactgcgt gagacaagtt cgccgcacgc tcggacggct gcatagcttc gatccgcgcg
     4261 acaaagaagc cgccagcatc tgggacaccg tcaagaacgc cgaagccaac cgcatgatca
     4321 tcgtgggcat ggaagttttc atgggcgcag ttggcgtggc cacgcttttc ctgggtggct
     4381 tgggcgttat gaacgtgatg ctcgtctccg tccgcgaacg cacccgtgaa atcggcgtgc
     4441 gcaaagcgct aggtgccact cgcgaatcca tcctccgtca attctttctc gagaccatca
     4501 tcgtcgtcgc cctgagcggt ggcacgggtc tgctcctctc ctacggtttt tgcgcgctgg
     4561 tgaatctgct gcccatgccg ctgttttttg cgggtttgct caccagctgg aagcttggcg
     4621 ctctgtccat ttttctgctc ggtttaattg ccgttctctc cgcgctctat ccggcgaatc
     4681 gcgccgcttc tgtcgatccc atcgaggcgc tgcgcttcga ggcgggtggc tagtcagccg
     4741 gttttctgtg gtcagtttta gttgtcagtg aagaaaggga gaggttgatt acggtgagat
     4801 ggacttcttc atcggaaccg ggttcagcca gggcgcgtgg cggtcgggcc ttgttccact
     4861 cgctggatca ggccagtaag catacgcttg atttcacgga cgctggtgtc gagagcctct
     4921 tgctgtttct cagtcagaaa acgcaggtcg cgagcgagca gcagaaagta ttcaagttcg
     4981 gttgcagatc cgacggagat gtaaaggaat tgccggagct ccgccggacc cgctcggccg
     5041 cagccctcag caatgttcgc gggcaccgac gcggcggcac gtctcatctg gctcgtcagg
     5101 ccgtatcgct ctggttctgg aaaggtggcg gtggcagagt agatccgcag aacgagggaa
     5161 tggctcgttt gccacacctt caattgtctg taatcttgca tgagagaaat aaccaagcaa
     5221 ctatattcct ctcttactta caactgaata ctgaaaactg tcaaccgctt ggagcagcca
     5281 gtgctaactg aaatcttcaa agaagcatgg gccgcacttg gccgcaatcc cgtccgcagc
     5341 ttgctcacga tgacgggcat cgcgtggggc atcgttgcgg tgacactctt gctcagctac
     5401 ggctccggct tccgcaatgt tttgatgtac acattcgaag tctttggcaa gggcgcggtg
     5461 gtttgttggc ccggaaccac cagtgagcag gctggcggcg aacgcgccgg caaggccgtc
     5521 cgcttcgaac aagaggacgc ggattgggtg aaagcacaat ccccgctcgt caagcgcgtg
     5581 acccgcgaga cggtgcgctg gaagggcatt acacacgacg cgcggctctc ggacaccgcc
     5641 atccgcggcg tctatcccga atacggcgaa atgcgcaatg aaattcccat ggaaggccgc
     5701 tggatctctc ccgaagatat cgccgagcgc cgacgcgtgg cttttctcgg cgctatcttg
     5761 cgcaggaaac tcttcagcgg cacgcccgcc attggcgaga ccgtgcgcat cgacggcgtc
     5821 aaattcaccg tcgtcggctc catggacaca aaattctccg acagcaacta tttcaccagc
     5881 gacgacgagt ccgtgtttat tccgtacacc acggcaggcg acttgtggga cgcacgctac
     5941 gccagcgtga tgctcttcga gccagtcgcg ccgaattttg aagctgacgc catgcagcag
     6001 ttccgcgcgg ccattgccaa ccgccagcac ttctcgccga acgacaagcg cgccatcacc
     6061 atgttcggcc gcgaagagtt caagcccatc tacgaaggaa tcacattggg catcgaagcg
     6121 ctgctctttt ttgttggcgc actcacgctg ggcatcggcg gcgtgggcgt gatgaatatc
     6181 atgctcgtct cggtcgacga acgcgtccgc gaaatcggcc tccgccgcgc gctcggcgcc
     6241 aagaaatcac acattcgctg gcagttcttg ctggaagcgc tggtcctgac gctggccgcc
     6301 ggtgtgatcg gcatgctact ctcggcggcc atcacttcag cgatcggcac gctgccgttc
     6361 ctcggccccg cctatgaaga cgacaccggc aaagtggaca tcaaactcac tctctccttc
     6421 gtcaccatga tgctgtctac cgccatcctg gtcgtcgtcg gcgtgatcag cggctggctc
     6481 cccgccatgc aagccgccaa gctcgacccc gtcgaagccc tccgctacga ataatccggc
     6541 aagaaagcac aacgcagagg cgcggagctc gccgagaaaa aatgaagcca gagaaagaag
     6601 ctgagccatt ttccggctcc gctctgcgct gcctctgcct cctcagcgtc tctgcgttat
     6661 attttctctt gaatttcttt cttacgtcag cccgaggata gcgtcgaaat acgccagcat
     6721 attcggcgag tcccactgct ggaagccgat gaatttgcgg gcgatcttgc cgtggcggtc
     6781 gataacgtac gtctccgggt acatcgaggt gccataggac tgggcgatgg gcgaatgatt
     6841 gtctctggtg agcggatcgc gataggtcgg gaaaactacg ccctgttcgc ggaggaattt
     6901 ttcgtaagcc gtggggtcct cgtcggcagc gacaccgagt atcacgccgt ttcgcgcttc
     6961 gatgtatttc tgcaggcggt ttagcgcggg agtctcctcg acgcacggtg ggcaccaagt
     7021 cgcccagaaa ttcagtatca ccaccttgcc cttcagatcc gtgagatgcc cgggcttccc
     7081 cgcgatatcc agcgggaagt ccttcgctgt cgtgcccgca atcgaggcct cgccctggcg
     7141 gtagctcggc atcgcaaaca gcaggattac acccagcgca gccgccagca ctccccaagc
     7201 cgcaattttt cgaaggattc tcatcgaatg ctatactaac tcaatctccc cattcgggaa
     7261 acgacccttc cgagcacaag acacttatgt ccagcgaagc acacaatcca tgcaccagcg
     7321 gaactctagt ctcagcgacg actgcgggct ccgtcacggc catcgttccg gcccggaatg
     7381 aggaggcggt catcgcggcg tgcattgcat ccctggcgaa ccagccggaa attgccgaaa
     7441 tcctggtggt aaacgatcaa tccacggacg ggaccgccag cgtcgtgcgc agtctcacgg
     7501 agaaaattcc aaacctgcgc cttttggaat ccaccggctt gcccgatggc tgggttggca
     7561 agaatcatgc cctctgggtg ggagtgcaac aggcaaagtg tgcctggctt ctcttcaccg
     7621 atgccgacgc ggagcacgag cgagattccg tttcacgggc gctgcgaatt gcgcagaaac
     7681 aaggggctgt tttggtctcg ttttctcccg agcaagtcac cgagacctgg tatgaaaaag
     7741 ctctgattcc ttttatctac cttcgcctgg caaaacgctt ctcctatgaa aaggtaaatg
     7801 atccgaactc acccgccgcg gccgccaacg gacagtttct gatgatgcgc cgcgatgtgt
     7861 acgacgccat cggcggtcat tccagtgtcg ccggcgaggt tttggaagac gtcgccctcg
     7921 ccatgcgcgt gaaggccgcg ggccaacgta tctcgttcgg ttctggcaaa ggcattgtcc
     7981 gcgtgcgcat gtaccgctcg ttcccggaga tgtggcaagg ctggaagaaa aatctctatc
     8041 gcttgatggg cggcaccccc tgggctgttg tccgcgaaat ggagtcaaac cttccctgga
     8101 ttccagtcct gctgatcctt gcaggactga agtacccttt cctgcttttc ctgggcgttt
     8161 tgctcttgat tgcccgccaa accagctacg gcctcgatct cgcccgtaac caatacccct
     8221 tctccttcat cttttattac gtgcccgccg tggcgctgta cctagctgtg ctctgggcgt
     8281 cctaccggag ccatgtaaat ggaagaatcc aatggaaagg ccgggagtac tccgtcggcg
     8341 ctcccgaatc ggcgcgataa gcgaacaccc atggttcttc tcacgcgaaa aatcgagttt
     8401 gcggcctcgc atctctacca caacccgaat ttctcggtcg aggagaaccg tcgaattttt
     8461 ggaaagtgca acaaccccca cggccacggg cacaattaca ccctggaagt aaccgttgag
     8521 ggcgagcctg atcccgtgac cggcatggtc ctggacctga aagaactaaa agaaattctc
     8581 gaacgcgaag ttatgcagcg aatggatcat cgtcacctca actacgaggt cgctgaactg
     8641 gccggacaga ttccgacctg tgaaaacatc gcccgggtaa tctggcaatt gctggaaccg
     8701 aaaatcacgc aaggccgcct gcatcgcgtg cggctctacg agtcgcccga cctcttcgcc
     8761 gattgctacc gcaacggcgc ccgcgcagcg ggt
//
LOCUS       DASYVO010000075        12096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_142002, whole genome shotgun sequence.
ACCESSION   DASYVO010000075 DASYVO010000000
VERSION     DASYVO010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 12096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12096
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142002"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VGR03_02415"
     CDS             <1..1010
                     /locus_tag="VGR03_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV1993162.1"
                     /translation="PHWSLPAVLLAMVLFAPGAIAQQPLPPSAPPPPPGQEPGVTREK
                     QEGYKIRRDVSLVVLHTSVFDERGRFVEELKEDSFRVLEDKVQQKLAVFKREDIPVSM
                     GLVIDNSGSMREKRARVNEAALTLVQTSNPEDEAFVVNFNDDFYLDLDKDFTSSLTDL
                     KEALERIDSRGSTALYDAIIGSLDHAKKGSRDKKVLLVVTDGSDNASRNSLEKTIHEI
                     QKSEVVVYTIGLIGTEEGKRERSRARKALKEIAEASGGLSFFPENVADVHNICVEVAH
                     DIRNQYTLAYYPSNSKRDGTFRTVQVEVSSARGHGKLTARTRRGYFAPALAGPASGSA
                     R"
     gene            1182..1904
                     /locus_tag="VGR03_02420"
     CDS             1182..1904
                     /locus_tag="VGR03_02420"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HEV1993163.1"
                     /translation="MRKSLWVVLVVAALFLLSVVLFESREGAFSSSYAAPQETSAPLQ
                     TVGESVTYNFDSDSAGAMPAKFHAARTGQGREGKWLVMADATAPSKPNVVAQTSTDKT
                     DYRFPLLISDGGSYKDLELSVKFKAVSGEVDRAAGLVFRLKDANNYYVVRANALEDNF
                     RLYHLVAGSRRQFAGANFKVTSGEWHELRVECVGNKIICYYDGARKIEATDGTFKDAG
                     KVGLWTKADSVTYFDDLKVTAK"
     gene            2030..2956
                     /locus_tag="VGR03_02425"
     CDS             2030..2956
                     /locus_tag="VGR03_02425"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HEV1993164.1"
                     /translation="MLRFMKKGMLLLLVGVCLSFSGVPAVAQDVPGDYQDVLKFLDRK
                     GDYKAGVLKLNIPRSDLKMTIQGVATPTPFGFGGWVALTKGTGGTDVMMGDLVLLQEE
                     VNPVMSALLENGIDITALHNHFFWDDPHVFYMHVHGRGKAADLARRVKPGLDVIGHVT
                     PAVVAPSSGTGTALDTERLAKIVGHAGEQSGAVFKITIGRDDLGMKEHGAAINARMGL
                     NTWAAFLGTQEDAVVAGDVAMLENEVTPVLKALRKNGLNIVAIHHHMTEDRPVVIFLH
                     YWGRGPAEKLATAFKAALDALGKGGPAHGMGR"
     gene            2981..4156
                     /locus_tag="VGR03_02430"
     CDS             2981..4156
                     /locus_tag="VGR03_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEV1993165.1"
                     /translation="MNTTAMSVEGGEAPPKLREFLLYFLHLGTFGFGGPIALAGHMQK
                     DLVEERKWVSSEDYVEGLAFSQLSPGPLAAQLAMYLGWVRAGTLGATLTGVVFVLPSF
                     VMVMVLAALYVHYGRLPWIQGVFYGIGAAVIAIIARSAYKLVRSTLKNDWLLWVLFAA
                     LAVTTAWKEAEIVWLFLLCGVVSLFIKAPPRWKLSHRAGPVFVGLGKLFTGALGPATA
                     ATLGTLFLFFLKAGAFVFGSGLAIVPFLYGGVVGEFHWLTERQFLDAVAVAMITPGPV
                     VITAGFIGYLVAGAIGALLAALAVFVPPYLIVIAGAPYYRRFAKNLQVKAFVQGVTAA
                     AVGAIAGAAFILGRRSLIDLPTVSIAVVTFGLLSFKKIPEPLLILAAGVAGLLLFKG"
     gene            4246..6516
                     /locus_tag="VGR03_02435"
     CDS             4246..6516
                     /locus_tag="VGR03_02435"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEV1993166.1"
                     /translation="MTTAFGRSRAFLGVERKVTAASGALFSVVLIPLYLLPWSVGARA
                     LPNGPRSVPQEGARRAEAVRVDHAPKLDGTLDDPLWQSAKPITDFRQREPHEGEPPTE
                     KTEVRILYTRHAVYFGIHCYDSEPSRIIATELRRDVSQDLDDHFEILIDSNHDRRGAY
                     VFEVNPLGTQSDGLIVEEQGGASGGDFDRGWDGVWTSEARITPNGWTATIEIPFTTLN
                     FTHSADVIWGLNLKRFNRRKNEEDLWSAYRRTFGITKVSEAGELTGITDIGSGRLFIV
                     KPYALATYDKQTGQNPKFPLTGGLDVKYGLRSNLVLNLTGNTDFADTEVDLTPLNLTP
                     FKIFIPEKRQFFLENAGVFNFDIGDQDQLFFSRQIGIDPVTGQQVPINGGAKLTGTIG
                     RMQLGVMDVNTRSSGPNPYANYAVARLKESLWAGSYIGVMGIDKRSGNLQDSFNQTGG
                     VDTRLVFFKNWLVDAHMAGTRSPGGPSGASDVGASLSYRSNWLDGIVERRKIGPNFNP
                     EVGFIERVDSNETYGDLTFKVRPKITGVRELQFEGFILHAPDTHNEVSTQEWQGTFRA
                     EFNNGAYTDNDIADVFTQRISTPFHIYKSVFIPNGLYHFARHQLTYGSGQDRRFTYSF
                     FERFGGFYGGTLNEFRVRANFRPNAKFSVSVSETWDRFRLPLPNGNFSVVLASLQANY
                     SFNRFLTFSSLLQMDTSNTQAVSANLRLRYNYRPDSDLYIIYNVGTQFASIAPANPPQ
                     IRETRFAVKYTYSFRP"
     gene            6880..7671
                     /locus_tag="VGR03_02440"
     CDS             6880..7671
                     /locus_tag="VGR03_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEV1993167.1"
                     /translation="MNKLLVRCCVMIFALVVGGFVLVAGGSAQDWNGFYFGGNAGGVK
                     GNSDVFTSTIFSPTGYFARSSIPAIAATGHPGLSPRGFTGGGQAGYNYQRRHWVLGVE
                     ADFGSMHVNDDFTTTGIYPCCAPTNFTITQTVSTDWLFTARPRIGLGNASVLVYGTGG
                     IAMTNLNYKENFIDTFATAHESASTSGLLMGWTAGGGVEFKVGSGGHWSLKAEYLFAD
                     FGTGLKTTSTNLTAFTPPIAFPTNVFTHQADLTTHIFRAGFNYRF"
     gene            complement(8029..9231)
                     /locus_tag="VGR03_02445"
     CDS             complement(8029..9231)
                     /locus_tag="VGR03_02445"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV1993168.1"
                     /translation="MKRFERVVWIVLDSVGIGPLPDAAEYGDTGRNTLGHIAESRPLA
                     LPQLARLGLGNIAPLAHISPATKPLGAYGKGATRSPGKDTTTGHWEMAGVWLDKAFPL
                     YPNGFPPELIEEFERQIGRKTIGNKPASGTEIMKELGDEHVRTGRPIVYTSGDSVFQI
                     AAHEDVIPIAELYRMCEIARKLLDGPHLVGRVIARPFNGPSGAYARTTRRHDYAVDPP
                     RPMLLDVLKERGAPVFGIGKIHDIYNGRGVEDYVTTRSNADGMEKIAEALRERPAGLI
                     FCNLVDFDMLFGHRKDVEGFARSLEEFDRMLAALLPQLRASDLLMITADHGCDPDPRW
                     ETTDHSREYVPILAHSPGGGAGVNLGIRSTLADMGQTIAENFGGKIPHGESFLNVFAG
                     RPFSAPGA"
     gene            9381..10322
                     /locus_tag="VGR03_02450"
     CDS             9381..10322
                     /locus_tag="VGR03_02450"
                     /inference="COORDINATES: protein motif:HMM:NF028425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HEV1993169.1"
                     /translation="MAQTDRSGRRSIGGAVFLIGLGVVFLIFQLRPELDPWPLLHQYW
                     PLILIFLGLGKMWDYFRLRNNPDPSARPRFSGTAIALLLLLVLFAVAAARGKHTSWDN
                     LRHDVKTLDRQGAQTVHANIVMPAGELRLSGGSSRLLDADFRYREAEGIPDVEYSVAN
                     GTGELNIRQDNFSHVHMRWGHTRNDWDLRFGKEVPLDLNLNLGAGQGQLHLRDVPLTR
                     LEVNMGVGQVDLDLTGERKRDLQATIHGGIGQATIRLPKEVGVRVHAAGGIGSIDVSG
                     LSRDGDDYVNDAYGKSPVTITLDVQGGIGQITLTLAR"
     gene            10751..>12096
                     /locus_tag="VGR03_02455"
     CDS             10751..>12096
                     /locus_tag="VGR03_02455"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993170.1"
                     /translation="MATHEAIPVAAEQDVIRIRGARVHNLRNIDVEIPHNAITVVTGV
                     SGSGKSSLAFDTIYAEGQRRYVESLSAYARQFLERMEKPDVDEITGIAPAVAIRQKAT
                     TRNPRSTVGTATEIYDYLRLIYARCGTTYCIKCGVEVRRDTPEEIANRVLALSPGRRF
                     YVLYRLRFPPTPGKPAPATGGRPATTAAGKLATTSAGNPAPASVGRGPATLAGKGAAA
                     ARKQPGASPEAVRQALLDLQKRGFNRVFQDGCVHEFATPETLLDVDFAKPVFALVDRL
                     SVHADSRSRLVDSIEICYREGGGEAILELVADNAGNAAERMVFNERFECKKCGIVYQE
                     PEPRLFSFNNPYGACPRCQGFGNTIDIDMNLVVPDPGKSLEEGAVEPWTKPRFRAVFT
                     EAKRWARSRGISTQAPWRQLSAEQRRLLLEGDRESGFGGVKGLFEWLERKKYKLHVR"
BASE COUNT         2437 a         3520 c         3649 g         2490 t
ORIGIN      
        1 gccctcactg gtctctgccc gcggttttat tggccatggt gctgttcgcg ccgggagcca
       61 ttgcgcagca gccgcttccg cccagcgcgc cgcctcctcc gcccggccag gaacccggcg
      121 tcacccggga aaagcaggaa ggctacaaaa tccggcgcga tgtcagcctc gtcgtcctgc
      181 acacctccgt tttcgatgaa cgaggccgct tcgtggagga gctgaaggaa gatagtttcc
      241 gcgttctcga agacaaagtt cagcagaagc ttgccgtctt caagcgcgag gacatcccgg
      301 tcagcatggg cctggtgatt gacaacagcg gcagcatgcg cgagaagcgc gcccgcgtga
      361 atgaagccgc gctcaccctt gtccagacca gcaatccgga agatgaggcg ttcgtcgtga
      421 acttcaacga cgatttttac ctggacctcg acaaggattt caccagcagc cttaccgacc
      481 tcaaggaggc ccttgagcgc atcgattcgc ggggaagcac ggcgctctac gacgccatca
      541 tcggctcgct cgaccacgcc aagaagggaa gccgggacaa gaaagtgctc ctcgtcgtca
      601 ccgacggctc agacaacgcc agccgcaatt cgctcgagaa gaccattcac gagatccaga
      661 aatccgaagt cgtcgtctac accatcggtc tgatcgggac ggaggagggc aagagagaga
      721 ggagtcgcgc tcgcaaggcg ctcaaggaaa ttgccgaagc ttccggcggg ctctctttct
      781 tcccggaaaa tgtcgccgac gtccacaata tctgcgtgga ggtcgcgcac gacatccgca
      841 accagtacac cctggcctac tatccgtcga atagcaagcg cgacggcacc ttccgcaccg
      901 tgcaggtgga agtcagttcc gcccgcggcc acggcaagct caccgcccgc acccgtcgag
      961 gctacttcgc ccccgcattg gctggccctg cctccggctc cgcacgttga cggcccgacg
     1021 aagggtcact cggggcggtc caacccaaaa gcgctcagcc gaacctgggc gccctgtttt
     1081 tcgtcactcc acgatcaatt ccagcggtgg tatcctagcg tcatcatctt cttgcctatc
     1141 aaagaatctt tcaactcgac actgagtcct ggaggaatac gatgagaaag agcctatggg
     1201 ttgtacttgt ggttgcagcg cttttcttgc tgagcgtcgt gctcttcgag tctcgggaag
     1261 gggcattttc ttcatcctac gcagctccgc aggagacatc ggcaccactt caaactgtcg
     1321 gagagtcggt tacctacaat tttgacagtg acagcgcggg ggcgatgcca gccaagtttc
     1381 acgctgcccg cacgggtcaa ggccgcgaag gtaagtggct ggtgatggcg gatgcgacgg
     1441 cgccctcaaa accaaacgtc gtcgcccaaa cctccaccga caagaccgac taccgttttc
     1501 ctcttcttat ttcggacgga ggaagctaca aggacttgga gctgagtgtg aaattcaaag
     1561 ccgtttccgg cgaagtggac cgggcggcag gcctggtctt tcggctgaag gacgcgaata
     1621 actactacgt cgtccgagcg aatgctttag aggataactt ccgtctctac cacctcgtcg
     1681 cgggaagtcg ccggcagttt gccggggcga acttcaaagt cacgtccggc gaatggcatg
     1741 aactgcgcgt cgaatgtgtc ggaaacaaga tcatctgcta ctacgacggc gccaggaaga
     1801 tcgaagcgac ggacggcacg ttcaaagatg ccggcaaggt cggactgtgg acaaaagcgg
     1861 attcggtgac gtacttcgac gatctgaagg tcacggcgaa atagactgag ggagatcatc
     1921 gatgaaccgg attacgacgc ggtgtaggag ttcgttcgcc ggcaggtgag ttgagcgcac
     1981 gcgactccgt tatggacaac cagctgttaa acaacaggag gttcgcttga tgcttcgctt
     2041 catgaaaaaa gggatgttgc tcttgcttgt tggtgtttgt ctcagcttct ccggcgtgcc
     2101 tgccgttgcc caggacgtgc cgggcgacta tcaggacgtt ctgaagttcc ttgatcgcaa
     2161 aggggactac aaggccggcg ttctgaagct gaatattccg cgctctgatc taaagatgac
     2221 gattcagggt gtggccactc ccacgccgtt cggtttcggg ggctgggttg cattgacgaa
     2281 agggacaggc ggtacggatg tgatgatggg tgaccttgtg cttctccaag aggaagtcaa
     2341 tccggtcatg tccgctctcc tcgagaatgg gatcgatatc accgcgctcc acaaccattt
     2401 cttctgggac gacccgcacg tcttctacat gcacgtgcac ggtcgcggca aagctgccga
     2461 cctcgcccgc cgcgtgaagc cgggcttgga tgttatcggg catgtcacgc cggcggttgt
     2521 tgctcccagt tcggggacgg gtacggccct cgacacggaa aggctggcaa agattgttgg
     2581 gcatgccggc gagcaatccg gggcggtgtt caagatcacc ataggacgcg acgaccttgg
     2641 catgaaggaa cacggcgccg cgatcaacgc tcggatgggg ctcaacacgt gggctgcatt
     2701 cctcggcacg caagaggatg ccgttgtcgc cggcgacgta gccatgctgg aaaacgaagt
     2761 cacgccggtc ctgaaagcgc tccgcaagaa cggtctgaac atcgtcgcga ttcatcatca
     2821 catgaccgag gatcgtcccg tggtgatctt ccttcactac tggggcagag ggcccgcaga
     2881 aaaactcgca actgcgttca aagcagctct cgatgcattg ggcaaaggcg gtcctgccca
     2941 cggaatgggc cgctagggtt ctcttacgcc cgaccgagag atgaatacga ccgcgatgtc
     3001 agtcgagggt ggagaggcgc cgccaaaact gcgcgaattc ctgctttatt ttctccacct
     3061 cggcactttt ggctttggcg ggccgattgc tctcgcgggc catatgcaga aggacttggt
     3121 tgaggagcgc aagtgggtat ccagtgagga ttacgtcgag ggactcgcgt tttctcagct
     3181 ttcccccggc ccgctcgcgg cgcaacttgc catgtatctc ggttgggtcc gggccgggac
     3241 gcttggcgcg acgctcacgg gagtcgtgtt tgtcctgcca tctttcgtca tggtcatggt
     3301 gcttgccgcg ctctacgtgc attacggccg gctgccttgg attcagggtg tcttctacgg
     3361 catcggcgcg gctgttatcg ccatcatcgc tcgcagtgcc tacaaactgg ttcgctcgac
     3421 gttgaaaaac gattggctgc tgtgggtgct cttcgcggcg ctggcggtca cgaccgcatg
     3481 gaaggaagcc gaaatcgtct ggctcttcct gctctgcgga gtcgtttcgt tgttcatcaa
     3541 ggcaccgccg cgatggaaac tttcacaccg cgcgggtcct gttttcgttg gactagggaa
     3601 gctgttcacg ggtgccctgg gaccagccac agcggctacg ctaggcactc tctttctgtt
     3661 ttttctgaag gcaggtgctt tcgtctttgg gagcggcctg gcgattgtgc cgtttctgta
     3721 cggcggggtc gtaggcgaat tccattggct gacagagagg cagttccttg atgcggttgc
     3781 agtagcgatg ataacaccgg ggccggtcgt catcacggcc ggattcatcg ggtatctcgt
     3841 agccggggcg attggagcgc tgctcgccgc actcgctgta tttgttcctc cgtatctgat
     3901 cgtgatcgcc ggcgctccgt attatcggcg ctttgcaaaa aacctccagg tgaaggcatt
     3961 cgtacaagga gtgacagccg ccgccgtcgg tgccatcgct ggagcggcct tcatccttgg
     4021 caggcgttcg ttgattgatc tgcccacggt ctccattgcc gtcgtaacgt tcggactgct
     4081 cagcttcaag aaaatcccgg aaccactttt gattctcgct gcgggggtag cgggtctgct
     4141 gcttttcaaa gggtagggat tgggaagtta tttgaagaaa tcgaggttta gagcttctcg
     4201 gaccatctca actccacttc ggagcgaatc cttttgggaa caaccgtgac aactgcgttc
     4261 gggcgatcaa gagcctttct aggcgttgag cgcaaggtca ctgccgcttc tggagccctg
     4321 ttctcagttg ttcttattcc cttgtacttg ttgccgtgga gtgttggagc tcgtgctctc
     4381 ccaaacgggc cccgttctgt cccgcaggag ggggcgcgca gagcggaagc ggtacgcgtt
     4441 gaccatgctc caaagcttga cggaaccctt gacgacccgc tttggcaatc cgccaagccc
     4501 attacggatt ttcgacagcg agagccgcat gaaggtgagc cgccaacgga gaagacggaa
     4561 gtgagaattc tctatactcg gcacgccgtg tacttcggga tccattgcta tgactcggaa
     4621 ccctcgcgga tcatcgcgac ggaactccgg cgcgacgtta gtcaagactt ggacgatcac
     4681 ttcgaaatcc tgatcgattc gaaccatgat cgccgtggag cctacgtatt cgaggtcaac
     4741 ccactcggca cgcaaagcga tggtctcatc gtggaggagc agggaggcgc aagtggaggc
     4801 gactttgacc gcgggtggga cggggtatgg acgtcagagg cgcgcatcac gcctaatgga
     4861 tggactgcaa caatcgaaat cccattcaca accctcaatt tcacgcactc cgcagatgtg
     4921 atctggggtt tgaatctcaa acgctttaac cgcagaaaga acgaagagga cctgtggagc
     4981 gcataccggc gaacgtttgg gatcaccaag gtctccgagg ctggagaact gacggggata
     5041 acggacattg ggagcggaag gctcttcatt gtgaagccgt atgcactggc cacctatgac
     5101 aagcagacag ggcagaatcc caagttccca ttgacgggcg ggctcgatgt caaatacggc
     5161 ctgcgctcaa atctcgtatt gaaccttacc ggaaatactg atttcgccga taccgaagtc
     5221 gatctgacgc ccttgaacct cacgccattc aaaatcttca ttccggaaaa gcgccagttt
     5281 ttcctggaaa acgccggggt cttcaacttc gacatcggcg accaagatca actgtttttc
     5341 agccggcaaa tcgggatcga cccggtgacc ggacaacagg tacccatcaa tggcggagcc
     5401 aagctaaccg ggacgatcgg caggatgcag ctgggcgtca tggatgtgaa cactcgatcc
     5461 agcggtccca atccgtatgc aaattatgcc gtcgcgcggt tgaaagaatc attgtgggcc
     5521 ggctcctaca tcggcgtaat gggcatcgac aagcgatccg gcaaccttca ggacagtttc
     5581 aatcaaaccg gaggcgtcga tacgcgcctg gtcttcttca agaactggct cgttgacgcc
     5641 catatggccg gaactcgatc ccccggagga cctagcgggg cgagcgatgt aggggcttcg
     5701 ctgtcctatc ggtcaaattg gctggacgga atcgtcgaaa ggcgaaagat cgggccgaac
     5761 ttcaacccgg aagtgggatt catcgagagg gtggattcaa acgaaacgta cggggatctc
     5821 actttcaagg tgagacccaa gatcacgggc gtccgcgagt tgcaattcga gggcttcatt
     5881 ctccacgctc ccgatacgca caacgaggtc tcgacccagg aatggcaggg cactttccgg
     5941 gcggagttca acaacggcgc ctacaccgat aacgatatcg cggacgtttt cacgcagcgg
     6001 atttcaaccc cctttcatat ttacaagagc gtgttcattc cgaacggcct gtatcacttc
     6061 gcccgccatc aactcaccta tgggtccggg caggatcgcc ggtttaccta cagtttcttc
     6121 gaacgctttg gcggcttcta cggagggact ctgaatgaat tccgcgtgcg ggccaacttt
     6181 cgcccgaatg cgaagttctc ggtctcggtt tcggaaacct gggatcgatt tcgactgcca
     6241 ctgccaaacg ggaatttctc cgtagtgctt gcgagcttgc aagcaaacta ctccttcaat
     6301 cggttcctga ctttctcgag tcttcttcag atggatacct cgaacaccca ggcagtcagt
     6361 gccaaccttc gcctgaggta taactaccgc cccgacagcg atctttacat catctacaac
     6421 gtcggaactc aattcgccag cattgctccg gcaaaccccc cgcaaatccg cgagacccga
     6481 ttcgccgtca aatatacgta ctcgttcaga ccttgagcct ggatgccatc cacgaattta
     6541 tcgtactcca acggtcagcg ctcgaactcc agcgagaacc gaatctgccg ggcaccgaaa
     6601 ccctcaactg gctgccccaa tccgaagatg gcttaggttc aagtgggcgg ggtacttgat
     6661 ctcgacctta tccgtctaag cgacgcgcac gccgtctccg cacacggcat cacactatgt
     6721 ccgcattctg tgagaggtct ccctcgggcg acgcagcccc gccctcgcgc gctttaccgg
     6781 gagtaaccat ttagcgccat ggcgccttcg agttgcagtc gaattacgtt ctgtgccata
     6841 atcccggaat cccgggggag taaattcgcg gaggaaaacg tgaacaaact tttggtgaga
     6901 tgttgtgtga tgatcttcgc cctcgtcgta ggtggttttg tccttgtcgc aggaggttct
     6961 gcgcaggatt ggaatggttt ttatttcggc gggaacgccg gaggcgtcaa gggcaattcc
     7021 gacgtgttta caagtacgat tttcagtccg accgggtatt tcgccagaag cagcattcca
     7081 gcgatcgcgg ccaccggcca tccggggctg agtccgcgcg gtttcaccgg cggcggccag
     7141 gctggctaca actatcagag gcggcattgg gttctgggag tcgaggccga tttcggctcc
     7201 atgcacgtga atgatgactt taccaccacg gggatttatc cctgctgcgc cccgacgaac
     7261 ttcacgatta cccagacggt cagcaccgat tggctgttca ctgcgcgccc ccgcatcggg
     7321 ctggggaacg ccagcgtcct ggtgtacggc acgggcggca ttgccatgac caacctcaac
     7381 tacaaggaaa actttatcga caccttcgcg accgcgcatg agagcgccag cacttcgggt
     7441 ttattgatgg gctggaccgc gggcggcggc gtcgagttta aagttgggag tggaggccat
     7501 tggtcgctga aggctgaata cctctttgcc gattttggca cgggattgaa gacaacgagc
     7561 accaatctca ccgccttcac cccgcccatc gcattcccca caaacgtctt cacgcaccag
     7621 gccgatctca ccacgcatat ttttcgcgcc ggcttcaatt accgattctg agctacggcg
     7681 gctggcgcaa cgcgccggcc gctctaaccc ccatagcgcg ctagggagag actgtttgtt
     7741 tggcgtcagt ggttcgccgt ctgcccactg ttcggcttgg atggggcggg attcttgatt
     7801 ttggcattat ccgtctatgc ggcgcgcacc ccgtctccgc gcacgccacc acacgatatt
     7861 cggattttgt taggggtctc cctcgggcgg cgcagccctg ccttccgcgc cggcggccgg
     7921 cgccggcgtg ggttgcggca cggattcagc cggatttccg acttgtcaaa aaagtttgtc
     7981 aagtaaaact cttttgacaa gtaaatcggc gcctaagtcc tttttttctc acgcccccgg
     8041 agccgaaaaa ggcctccccg cgaacacgtt caggaagctt tctccgtgcg gaatttttcc
     8101 gccgaaattc tccgcgatgg tctgtcccat gtcggccagg gtggagcgaa ttcccaggtt
     8161 gactcccgcc ccgcctccgg gcgagtgcgc gagaatggga acgtattcgc gcgagtgatc
     8221 cgtggtctcc cagcgcgggt cgggatcgca gccgtgatcg gcggtgatca tcagcagatc
     8281 cgaggctcgc aattgcggca gcaacgcagc gagcatgcgg tcgaattctt ccagcgaccg
     8341 cgcgaagcct tcgacgtcct tgcggtggcc gaagagcatg tcgaaatcca ccaggttgca
     8401 gaagatcagc ccggccggtc gttctcgcaa cgcctctgcg attttttcca ttccgtccgc
     8461 gttgcttctt gtcgtgacgt aatcttccac gccgcggccg ttgtagatgt cgtggatttt
     8521 cccgatcccg aagaccggcg cgcctcgttc tttcagcaca tcgaggagca tcgggcgcgg
     8581 cgggtcaacg gcgtagtcgt gccggcgcgt ggtgcgagcg taggcgccgc tcggtccgtt
     8641 gaacggcctg gcgatcacgc gcccgacgag gtgcggcccg tccagcaact tccgcgcgat
     8701 ttcgcacatg cggtaaagct cggcaatggg gatcacgtcc tcgtgcgccg caatctgaaa
     8761 aacgctgtca ccggaggtgt aaacaatcgg ccggccggtg cgcacgtgtt catcgccaag
     8821 ctctttcatg atttcggtgc cggaggcggg tttgttgccg atcgtttttc gaccgatctg
     8881 tcgctcgaat tcctcgatca attcgggcgg aaaaccgttc ggataaagcg gaaacgcttt
     8941 atcgagccac accccggcca tttcccagtg gcctgtcgtc gtatctttcc cgggcgaacg
     9001 agtggcgccc ttgccgtaag cgccgagcgg tttcgttgcg ggcgaaatgt gtgcgagcgg
     9061 cgcaatgttg ccaagtccga ggcgggcgag ctggggcagc gcgagcggcc gcgattccgc
     9121 aatgtggccg agcgtgttgc gcccggtgtc gccgtactcc gccgcatcgg gcagcgggcc
     9181 gatgcccacg ctgtccagca cgatccacac gacgcgctcg aatcgcttca tcacaggctg
     9241 ctcacggaaa actttaacac acgcctcgaa gaagcgcgca aacctactgg ggcgcagaag
     9301 gctggagagc tgccgcgaga ttcaggaacg tgaaactatt cgagaagcat ttgcgtacac
     9361 taggagcagg agggtgtgtc gtggcgcaaa cagacaggtc gggacggcgg tccatcggag
     9421 gcgcagtttt tctgatcggg ctcggcgtcg tcttcctcat tttccaactt aggccggaac
     9481 tggatccctg gccgctcctc caccaatact ggccgctcat cttgatcttc ctgggcctcg
     9541 ggaagatgtg ggattacttc cggctgcgaa acaatcccga tccctccgca aggccgcggt
     9601 tttcgggcac ggcgatcgcg ctgcttctct tgctggtcct gttcgccgtg gcggccgcga
     9661 gagggaaaca cacgagctgg gacaatctcc ggcacgatgt gaagacgctg gatcgccagg
     9721 gcgcgcaaac cgttcacgcg aacattgtca tgcctgcggg agagctgcgg ctgagcggcg
     9781 gctcgagccg tttgctggac gccgattttc ggtaccggga ggcggagggc attccggatg
     9841 tggaatacag cgtcgcgaac ggaacgggag agctgaatat acggcaggac aatttcagcc
     9901 acgtgcatat gcgctggggc cacacgcgga atgattggga tctgcgattc ggcaaggaag
     9961 ttccgctcga tctgaatctg aacttgggag cgggccaggg gcaactgcac ctgcgggatg
    10021 ttcccctcac gcggcttgaa gtgaatatgg gtgtcggtca ggtggatctg gatctgacgg
    10081 gcgagagaaa gcgagacctg caagcgacga ttcacggcgg catcggacaa gccacgatcc
    10141 gtcttccgaa ggaagttggc gtgcgcgttc atgccgcggg cggcatcggc tcgattgacg
    10201 tgagcggatt gagccgcgac ggcgacgact acgtcaatga cgcctacgga aagtctccgg
    10261 tgacgatcac cctggacgta caaggcggca tcggccagat taccctcaca ttggcgcgct
    10321 gagccgcgca cagtggctcc gttaagcgct gttcgggaag cgaaccgagg tcaaggatgc
    10381 gcacgatcgc tacgcgaatc tggaaaccaa ctaccttctg cgaaggatcg aggaatatgg
    10441 acggcccgtg gtcctcacca tgaacctgcg gaaacgaatc gatagcgcat tccccagagc
    10501 tatctgtgcg ataaggccct aatacagcgg ggctgtgatt tagaggttcc cggcgaaacc
    10561 tagtgagagc cgcccacaaa aaggtccagc aggcagatca cccgcacatt ggcggaggaa
    10621 gccactcgcg ataatcttct gcgcttttca cctcgttgct ttgccctctg cgtttgcggc
    10681 gctttccgcc gggacgtata atttagagtt tgggcagggc gcgcggagcg cgctgtccgg
    10741 gcttcctcac ttggcgaccc acgaagcgat acccgttgcg gctgagcagg acgtgatccg
    10801 catccgcgga gcgcgcgtcc acaacctgcg gaacatagac gtcgaaatcc cgcacaacgc
    10861 catcacggtt gtaactggag tctccgggtc ggggaaatcg agcctggcct tcgacaccat
    10921 ttacgcggaa gggcagaggc gctacgtcga gtcgctctcg gcttatgcgc ggcagttcct
    10981 cgagcgcatg gaaaagccgg acgtggacga gattacgggc attgcgcccg cggtggccat
    11041 ccggcagaaa gcgacgacga ggaatccgcg ctcgacggtg gggaccgcga ctgagattta
    11101 cgactacctg cgcttgattt atgcgcgctg cggaaccacg tactgcatca agtgcggcgt
    11161 ggaggtccgc cgagacacgc ccgaggaaat cgccaatcgc gttctggcac tgtctcccgg
    11221 ccggaggttt tacgttctgt acaggctgcg tttcccgcct acgccgggca agcccgcccc
    11281 ggctacgggg ggcaggcccg ctacaactgc ggctggtaag ctcgctacaa cttctgctgg
    11341 gaatcccgcc ccggcttcag tgggcagggg cccggccacg ctggcgggaa agggcgctgc
    11401 ggcggcgaga aaacagcccg gcgcgtcgcc agaagcagtt cgccaggcgt tgctcgacct
    11461 gcaaaagcgc gggttcaacc gtgtgtttca ggacggctgc gtgcacgagt tcgccacgcc
    11521 ggagacgctt ctcgacgtgg atttcgcgaa accggttttt gcgctggtgg accggctgag
    11581 cgtccacgcg gattcccgct cgcggctggt ggattcgatc gagatctgct atcgcgaagg
    11641 cggcggcgag gcaattctcg aactcgtcgc ggacaacgcc ggaaacgcgg ccgagcgcat
    11701 ggttttcaac gagcggtttg aatgcaagaa gtgcgggatc gtctaccagg agccggagcc
    11761 gcggctgttt tccttcaaca atccgtacgg cgcgtgcccg cgatgccagg gattcggcaa
    11821 caccattgat attgatatga acctggtggt gccggacccg gggaagtcgc tggaggaggg
    11881 agcggtcgag ccgtggacga agccgcgatt ccgcgccgtg ttcacggaag cgaagcgctg
    11941 ggcgcgctcg cggggaatct ccacgcaagc gccgtggcgg cagctcagcg cggagcagcg
    12001 ccggctgctg ctggaaggcg atcgggagag cggcttcggc ggcgtgaagg gcctgttcga
    12061 atggctcgaa cgcaagaaat acaaattgca cgtgcg
//
LOCUS       DASYVO010000076         4817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_157942, whole genome shotgun sequence.
ACCESSION   DASYVO010000076 DASYVO010000000
VERSION     DASYVO010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157942"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            413..2290
                     /locus_tag="VGR03_02460"
     CDS             413..2290
                     /locus_tag="VGR03_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1993171.1"
                     /translation="MASLLSPQLKRLLGYVRPYRFRMSMGVVLLAIVALAEGAVTLLI
                     VPIFDRVLAPASSDSNILLFRVPFGGPPVYVNHLFPHSIHNVWTVVSISMLVLFGVKS
                     LCEFGGVSLIQYVGHRAITDLRNAVYSKIIRQPIGFFQHQSAGRVFSAVINDVERARL
                     AISEYLAELFRQTFLFPVFLSILLYIDWRMTLACGVFLPLIVWPIGKLGRRIRRSVET
                     SQTRLGELTQILQETVGGNRIVKAFGMEDFEIGNFQAASKRLLRETMRWVRAQVATSP
                     LMDLLQPIVIALLLLYARDRIRLNMMTTGMFIAFVYALFKSYEPIKRIGAVYQQFQQA
                     QGATTQVFAYLDLAEEEQDVPGAVELPAFAREVQYDDVSFSYDAAPVLRNIRFSARKG
                     EVVAFVGTSGAGKTTLVNLIPRFYDVTSGALRIDGLDVRRARLRSLREQIAMVTQENI
                     LFHDTVWNNISYGLTDIPGEKVIAAAQAALAHDFIMELPQGYSTVIGERGTRLSGGQR
                     QRIAIARAILKDSPILILDEATSELDAESEMFVQKALANLMVGRTTFVIAHRLATIRR
                     ADRILVLEDGQIRESGTHTELMARGGTYARLHDLQFADDDMLAPAQAAPAGLNVASDS
                     L"
     gene            2287..3225
                     /locus_tag="VGR03_02465"
     CDS             2287..3225
                     /locus_tag="VGR03_02465"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEV1993172.1"
                     /translation="MTQTTTAPTKKIECFSMTGYAQARAEQDGWAVRVSVKSVNHRFL
                     DLKLKMPEGFDLYDLRLRQIVRERIHRGHVEVTVAVELGKAAPVQVNRELVQSYLKAA
                     EALRQETRAATDMDVVALLRLPGVITGVTTSMPETEEEQERLARVLDGGLRDALAKLD
                     DMRRAEGRHLVEELRDRLARIGGHVEQVRGLAATLRPAFSRRLDAKLKELLGGIGIDP
                     ARLAQEAALLAERSDISEELDRLRSHLQQFTKLLDGAGELGKKLDFLLQEMHREANTM
                     LSKTPGVESEALAITGVALEIKAEIEKLREQVQNIE"
     gene            3222..3857
                     /gene="gmk"
                     /locus_tag="VGR03_02470"
     CDS             3222..3857
                     /gene="gmk"
                     /locus_tag="VGR03_02470"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV1993173.1"
                     /translation="MSTEIEIAPLVLIVSGPSGSGKSTLVQRILELPGTMPSISCTTR
                     ARRATEASGKCYDFVTEAEFDAMVARGEFLEYARVFGKHSYGTPGKWLEESRKKGLDL
                     VLEIDVQGAAQVKEKLPESVAIFILPPSREELERRLRDRGQDSSEEIARRLAKARDEI
                     AAFGKYYDFCVVNEAVERAGREVQAIVTALHCTSARRRARVEQLLASFGRE"
     gene            3854..4090
                     /gene="rpoZ"
                     /locus_tag="VGR03_02475"
     CDS             3854..4090
                     /gene="rpoZ"
                     /locus_tag="VGR03_02475"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV1993174.1"
                     /translation="MTDMTMQTKAPQSQFAYVVLAARRARQIMAGAPPLIENPRTLKA
                     TRIACEELENSLLEYDFTEPASIDVDKEGNKRRK"
     gene            4110..>4817
                     /gene="coaBC"
                     /locus_tag="VGR03_02480"
     CDS             4110..>4817
                     /gene="coaBC"
                     /locus_tag="VGR03_02480"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEV1993175.1"
                     /translation="MRITLGVTGGVAAYKAAELVRRLQQDGFSVQVVMTRGAREFVTP
                     LTFAALSGQKVITDLFGDSSGGEANLESAIEHIAVAQRTDLLLVAPATADILAKFARG
                     IADDFLTTLYLASTAPVVVAPAMNVNMWNHAATQENVDMLRARGVKIVDPAEGYLACG
                     MTGAGRLAGQDDIAAAVREALKTQQDLDGETVLITAGPTCEDLDPVRYITNRSSGKMG
                     YAVAEAAALRGAKVILVS"
BASE COUNT          988 a         1310 c         1565 g          954 t
ORIGIN      
        1 tttgtggttt agtttgtgcc gggatgtcac tagctgaccc gaacgaattg cctctcagtt
       61 acgccgcgac cctctggcca atgttgacga ccgtgatctc ggttgaccga ggagatgcct
      121 acccccgggg ggtggaatat ccagtagtcg atcttgtgat cgctaggcta atcaacgccg
      181 ttggacttga ggagctggac ccaaagtttt tccccggacg tgtgttgccc cgaactgaaa
      241 ggtgaattga gtgctgcaat tctctgaaat cctaggagtt ggcaggaagt tttttcattc
      301 ggccgactaa cgcgcaaact cgaaactaac ccagtaccgg gggccgcgga aacttgacac
      361 gccgcgacgc ggcgaatagc gtaccaagtg gacaagtcag ggagcctgta gcgtggcctc
      421 gttgttatca ccacaattga aacggcttct cgggtacgta cggccctacc ggtttcgcat
      481 gagcatggga gtcgtgttgc tggcgatcgt ggccctggcc gaaggcgccg tcacgctgct
      541 catcgtgccc atttttgacc gcgtcctcgc ccccgccagc agtgattcca atattttgct
      601 cttcagggtg cctttcggag gcccgccggt ctacgtgaac catctcttcc cccacagcat
      661 tcacaacgtc tggacggtgg tttccatttc gatgcttgtg ctgttcggag taaagtccct
      721 ctgtgagttt ggcggagtaa gcctgattca gtacgtcgga caccgcgcga tcacggattt
      781 gcgcaatgcg gtctactcga aaatcatccg ccagcccatc gggtttttcc agcatcaatc
      841 cgcgggaagg gtgttctcgg cggttattaa tgacgtggag cgcgcgcgcc tcgccatttc
      901 cgagtacctc gccgagctgt ttcggcagac ctttttattt cctgtcttct tgtctatctt
      961 actctacatc gattggagga tgaccctggc ctgcggcgtc ttcctgcctt tgatcgtgtg
     1021 gcccatcggc aaattgggac gccggatcag acgttcggtg gaaaccagcc agactaggct
     1081 cggcgaacta acccaaatcc tgcaggagac cgttggcggc aaccgcatcg taaaagcctt
     1141 tggcatggaa gacttcgaaa tcgggaattt tcaagcggct tcgaaacgtc tgctgcgcga
     1201 aaccatgcgc tgggtccgcg cccaggtggc cacctcgccg ctcatggatt tgcttcagcc
     1261 cattgtcata gcgctcctgc ttctctatgc ccgcgacaga atacggctga atatgatgac
     1321 cacgggaatg ttcatcgcct tcgtttacgc gcttttcaag tcctacgaac ccatcaagcg
     1381 aatcggcgcg gtgtatcagc agttccagca ggcgcagggc gcgaccaccc aggtcttcgc
     1441 ttatctcgat ctcgctgaag aagagcaaga cgttcccggc gctgtcgagc ttcccgcctt
     1501 tgcccgcgag gtccagtatg acgacgtcag tttttcttac gacgcggccc ccgttttgcg
     1561 aaatattcgt ttcagcgccc gcaagggcga agtcgtcgcc ttcgttggca ccagcggtgc
     1621 gggcaagact acgctcgtca atctgattcc gcgtttttac gacgttactt ccggcgccct
     1681 gcgcatcgat ggtctcgacg tccgaagagc aagactccga tctctgcgcg aacagatcgc
     1741 catggtgacg caggagaaca tcctgtttca cgacaccgtg tggaacaata tttcctacgg
     1801 gctgacggat atccccgggg agaaagtcat tgctgccgcg caagccgcgc tggcgcacga
     1861 tttcatcatg gagctgccgc aggggtatag caccgtgatt ggcgagcggg gaacgcgctt
     1921 gagcggggga cagagacagc gcatcgccat cgcgcgcgcc atcttgaaag attcgcccat
     1981 tcttattctg gacgaggcca cctcggagct ggatgcggag tccgagatgt tcgtgcagaa
     2041 ggcgctcgcc aacctgatgg tggggcgaac cactttcgtg atcgcgcatc gcctcgccac
     2101 gattcgccgc gcggacagga ttctcgtcct ggaagacgga caaatccggg aaagtggaac
     2161 gcacacggaa ctgatggccc gtggcggaac gtatgcccgg cttcacgatt tgcagtttgc
     2221 ggacgacgac atgctggcgc cggcgcaggc cgcaccggca gggctcaatg ttgcatcgga
     2281 ttccctatga cccaaacaac tacggcacca acgaagaaaa tcgaatgttt ctcgatgacc
     2341 ggctacgcgc aagcgcgcgc cgagcaggat ggctgggcgg tgcgcgtcag cgtgaagagc
     2401 gtcaaccaca ggttcctgga cctcaagcta aagatgcccg agggatttga tctgtacgat
     2461 ctgaggttac ggcagatcgt gcgcgaaagg attcatcgcg ggcacgtgga agtgaccgta
     2521 gccgtggaac tggggaaagc ggcgccggta caggtgaata gggaattggt gcaatcatat
     2581 ctgaaagccg ccgaagcact gaggcaggag acgcgagcgg caacggacat ggatgtggtc
     2641 gcgcttttga gattgcccgg agtgatcacc ggcgtcacta cctccatgcc ggagacggaa
     2701 gaagagcaag agcggcttgc acgagtgctg gacggcggct tgcgggatgc gctggcaaag
     2761 ctggatgata tgcggcgggc ggaaggacgt catctggtcg aggagttgcg cgatcgcttg
     2821 gcgcgcatcg gcggtcatgt ggagcaggtg cgcgggctcg cggcgacatt gcgcccggcg
     2881 ttctcacgtc gtctcgatgc caagttgaag gaactgctcg gcggaatagg catcgatccg
     2941 gcgcggctcg cgcaggaggc ggcgctgctt gcggagcgca gtgatatcag tgaagaattg
     3001 gaccgcctgc ggagccacct gcagcagttc actaaactgc tggatggtgc cggggagctg
     3061 gggaagaagc tggattttct tctgcaggag atgcatcgcg aggcgaacac catgctctcg
     3121 aagacgccgg gcgtggaaag cgaagcgctg gcgatcacag gagtggcgct ggaaattaag
     3181 gcggagatcg aaaaactgcg ggagcaggtg cagaacatcg aatgagcacg gagattgaga
     3241 tcgcgccgct ggtgttgatc gtgtcggggc cgtcgggatc gggaaagtct actttggtgc
     3301 agcgaatcct ggagctgccc ggcacgatgc cttcgatctc atgtactaca cgggcgcgcc
     3361 gcgcgaccga ggccagcggg aagtgttatg atttcgtcac ggaagcggaa ttcgacgcca
     3421 tggtcgcgcg cggcgaattt ctggagtacg cgcgggtttt tggaaaacat tcttacggca
     3481 cgccggggaa gtggctggag gaatcgcgga agaagggact cgatctggtg ctggagatcg
     3541 acgttcaagg cgcggctcag gtgaaagaaa agctcccgga atcggtggcg attttcattt
     3601 tgccgccgtc gcgcgaggag ctggagcgca ggctgcgcga ccgcggccag gattcgagcg
     3661 aagagatcgc gcggcggctg gcgaaggcgc gcgacgagat tgcggccttt ggcaagtatt
     3721 acgatttttg cgtagtgaat gaggctgtgg aacgcgcggg acgcgaagtg caggcgattg
     3781 tgaccgccct gcactgcacg agcgcgcgac ggagggcgag agtggaacaa cttctggcat
     3841 cgtttgggag ggaatgacgg atatgaccat gcagacgaaa gctccccaga gccagtttgc
     3901 gtacgtggtg ctggcggcaa ggcgggcgcg gcagattatg gcgggtgcgc cgccgctgat
     3961 cgaaaaccca agaacgctga aggcgacgcg gatcgcgtgc gaagagctgg agaacagcct
     4021 gctcgagtac gatttcacgg agccggcgtc gatcgacgtg gataaggaag ggaataagcg
     4081 gcggaagtag cgggccgcga aaggcagcga tgcgaatcac gcttggtgtg accggcggag
     4141 tggcggctta caaagccgcg gaactcgtgc gccgtttgca gcaagacggg ttcagcgtgc
     4201 aggtggtgat gacccgcggg gcgcgggagt tcgtcacgcc cctgacgttc gcggcactga
     4261 gcgggcaaaa agtcattacg gatctcttcg gtgattcttc cggcggtgaa gcaaacctcg
     4321 aaagcgctat cgaacacatc gcggttgcac agcggacgga cctgctcctg gtcgcgccgg
     4381 ccacggcgga catcctggca aaatttgcgc gcgggatcgc ggacgatttt ctgacgactc
     4441 tgtacctggc atcgacggct ccagtggtag ttgcgccggc catgaacgtg aatatgtgga
     4501 atcacgcggc cacgcaagaa aatgtcgata tgttgcgcgc gcgcggcgta aagattgttg
     4561 accccgccga gggctacctc gcgtgtggca tgaccggcgc gggacggctg gcggggcagg
     4621 atgacatcgc cgccgccgtg cgcgaggcgc tgaaaacgca gcaggatttg gacggagaga
     4681 cggtcctgat aaccgcaggc ccgacttgcg aagacctcga tccggttcgc tacatcacca
     4741 accgctcgtc tgggaagatg ggctatgcgg tcgccgaagc agcggcactg cgaggcgcga
     4801 aggtcattct tgtgagc
//
LOCUS       DASYVO010000077         9515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_185025, whole genome shotgun sequence.
ACCESSION   DASYVO010000077 DASYVO010000000
VERSION     DASYVO010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9515
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185025"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="VGR03_02485"
     CDS             complement(<1..1293)
                     /locus_tag="VGR03_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993176.1"
                     /translation="MGIDSTKFQSFFDKLMKIKLFGSAPPPPPFGKKPTVAKLQKAIT
                     PGAQLLPTVFRDGYATPLQQNLTQVLAQLGQDLTSIEAITGAVYQQAQKVGQPQLHRF
                     LAVISDLYRSFLSKKRRTAANFPLVETVPPLAMFQNRGDSGPFTIPSDDIEKLMGAAI
                     GVVSMPAVYRDHPVLWTALSHETGGHDVIHSDVNLMPEMRAGVRQALGGPATITSSTN
                     LTQKQIIALLWDYWMDEAAADIYGVMNVGPMFGHNLTVFFAALNAQASKSNTPSLRTQ
                     SGFDPNDPQQLLDSHPTDLLRLSLIQGAVESLTGLAKGTTANYSADYDALAKLCAPAA
                     TKIDIAGNLLLGPSTRVPVQASFPLADMQAAAFKAGAFIATAKFNALSQHSIQDIETW
                     DDLDEQTAQSIATALAKNTSVAGLGDDAQLLAGANIAIF"
     gene            complement(1566..3245)
                     /locus_tag="VGR03_02490"
                     /pseudo
     CDS             complement(1566..3245)
                     /locus_tag="VGR03_02490"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            complement(3276..5249)
                     /locus_tag="VGR03_02495"
     CDS             complement(3276..5249)
                     /locus_tag="VGR03_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEV1993177.1"
                     /translation="MKPIHCVRTLTLLAPMLGVLLIADSSAAQAPPASAPLTLPSETP
                     AQFTPVTDSFDYARRDVMIPMRDGVKLHTVILVPKGARNAPILLTRTPYDATDLTTHS
                     HSSHLGPSLHGYDNALEVILEGGYIRVVQDIRGKYGSEGDYVMNRPLHGPLNPTMVDH
                     ATDTYDTIDWLVKNIPESNGKVGILGISYDGFTPLMALINPHPALKVSVPMNPMVDGW
                     MGDDWFHYGAFRELNMPYIYEQEATRKNDEHWWNSHYDDYDMYLQASSAGELGRRHGL
                     EQIGFWRKLLDHPAYDAFWQQQAMDKILATQPLKVPTMLVSSLWDQEDIYGAMAVYKV
                     LKPKDTGNDKVFLVMGPWHHGQEIGDGGSLGALKFGSDTGLYFRREILAPFLAHYLKD
                     DAPKSDVAPVAAFETGTNIWRRLPAWPSGCASGCAVKPTPLYLNAGLKLSFTAPAASE
                     APFDEYVSDPAKPVPFRARPSQPVGYSNGLTWSQWLVDDQREASGRPDVAVFVSDTLS
                     ASVKISGQPIANLIASTSGTDSDWVVKLIDLYPDEVGAQPEMGGYQLMVAADIFRGRY
                     RESFETPKALAPDKPLLYRFALPTANHVFLPGHRIMVQIQSSWFPLYDRNPQTFVPNI
                     FWAKPGDYRKATQRVYHVPGQATFVELPIVTAP"
     gene            5494..7293
                     /locus_tag="VGR03_02500"
     CDS             5494..7293
                     /locus_tag="VGR03_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV1993178.1"
                     /translation="MATATLTKPAAKGGSFLLESPQPGDVFTPADLTDDQRLIGQTAE
                     EFVLKEVFPFIKELENKKPGLMAELVKKGGEVGLMGGGVPEEYGGAGLDKISTTVLTE
                     KLSIYGGYAVTHGAHAGIGTLPIVYFGTEAQKKKYLPKLATGEWIGAYCLSEPQAGSD
                     AQNSLTRAELNAEGTHYVLNGQKMWITNGGFADVYIVFAKIDGEKFTAFIVERTFPGF
                     KPGNEEHKMGIHGSSTTPIFLENCKVPKENLLHEIGRGHIVAFNILNAGRFTLGASCV
                     GGSKHVLMTSSKYSKERKAFGKQIGDFGLMKEKLAEMAIQIFAVESMVYRSAGNIEAA
                     MAAASGSGDKIQNTMKVLEEYAIESSIAKVYGSEMLDFVVDEGVQIFGGYGFHEDYPV
                     CRAYRDSRINRIFEGTNEINRMLIIQMLMKRAMGGQLPLIPAAMKLADEILAGPSFEE
                     APEGVLAAETRVVANTKKMFLQAAGGAVQKFREKLADEQELIGALSNVVMEIYAMESC
                     LLRAQKAAAAKGESATQTMIDAARVFIHDAAERVEHEAKRAIAAIHEGDMLTTQMAVL
                     KRFAKRAPVDTIALRRRVAAAVQSQDRYPLEGR"
     gene            7463..>9515
                     /locus_tag="VGR03_02505"
     CDS             7463..>9515
                     /locus_tag="VGR03_02505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV1993179.1"
                     /translation="MPRKPLILAAVIVALHLCEAATLGTTAAGSLLANLLEIFACGFA
                     AVMAFGASRRGRGLSRPFWLIFGAGIAMWGVANLGWMYYEVVLHTEPPTTSAVRFLFG
                     LESVLIAMALFLDQDKDSPRIDAESALDFIQIGIVFFFIYVEFYYLPAHRLDNYSAFL
                     REMRVENVEDALLTVLAAFQALRARKQHIRKLYGGLAIYMLLLSVCAALAQYLQSIKP
                     APTGTLRDLLWTLPFLVVAMWAAQWQPSPAAETGSRLRRKTFGELMLTNATFVLAPLI
                     ILWQVSQLEAEWRLLRFSLLGISIVCFAARLGISQYREAKSSNAVMTHTLAMDSAVNG
                     MAILDARGKYIYVNPAYARMIGNTNREALLGKSWREVSHGRTVTPVESEIRKALMQHG
                     KWFGPLTVLHSDGTVVPMEMAITSLPEGGTICVSRDITQRVSAQRARAETEIKYRMLV
                     EQVAAVSYIAELGVNGQWLYVSPQVETMFGFSAEEWLSNSRDWIRHIPIEDHPIVNAA
                     EEASGRGEPFQAEYRITRKDGTTIWVSDTAVVVRGSDSHPVMEGLIVDITDRKMLENQ
                     LLQARKMEAVGRLAGGVAHDFNNLLTIIKGYVEMALQRCLDRPELHGDIRRIDDAADR
                     AVTLVRQLLAFSRKQVLRPKILDLNAIVVNLDQLLRRLMNENIEMKTFVSKDVGAIKA
                     DP"
BASE COUNT         1994 a         2539 c         3060 g         1922 t
ORIGIN      
        1 aaagatggca atattcgctc cagcgagcaa ctgcgcatca tcgccgagcc ccgctacgga
       61 agtatttttc gccagcgccg tggcgatgct ttgcgccgtc tgttcgtcga ggtcatccca
      121 ggtttcgatg tcctgaatcg agtgttgtga aagcgcattg aatttggcgg tagcgatgaa
      181 agcacccgcc ttgaaggccg cggcctgcat atccgccagc gggaaggaag cctgcacagg
      241 cacgcgagta ctcggtccga gcaggagatt cccggcaata tctatcttcg tggcggcagg
      301 agcgcaaagc ttcgccaacg catcgtagtc cgcggaatag tttgcggtcg tgcctttcgc
      361 cagccccgtc aggctttcca ccgcgccttg gatcagcgaa agtctgagaa ggtccgtggg
      421 gtgcgagtcc agcaactgct gcggatcatt ggggtcgaac ccggactgcg tccgcaacga
      481 aggcgtattc gactttgagg cctgggcatt gagcgcggcg aagaacacgg tgaggttgtg
      541 cccgaacatc ggtccgacgt tcatcacgcc atagatatcc gcggcggcct cgtccatcca
      601 gtagtcccac agcagagcga tgatttgctt ctgcgtcaga ttggtcgagg aggtgatggt
      661 cgctggtcca ccgagcgcct ggcgtactcc cgcgcgcatc tccggcatga ggttgacgtc
      721 ggagtggatg acgtcgtgcc cgccggtttc atgggagagc gcggtccaga gcaccggatg
      781 gtcgcgatag accgcaggca tgctgaccac gccgatagcg gcccccatca acttctcgat
      841 gtcgtcgctc ggaatggtaa aagggccgct gtcgcctctg ttctggaaca tggcgagagg
      901 cggcaccgtc tccacgagcg ggaagttcgc ggccgtacgg cgtttcttcg ataagaagga
      961 acggtacagg tcggaaatca ccgccagaaa tcggtgcaac tgaggttggc cgactttctg
     1021 ggcttgctgg tagaccgctc cggtaatcgc ttcgatgctg gtgagatctt gccccaactg
     1081 ggccaaaact tgcgtgagat tctgctgcaa tggcgtggcg taaccgtccc gaaaaacggt
     1141 gggcaagagt tgcgcccccg gtgtaatggc cttctgcagc ttcgccaccg tgggcttctt
     1201 gccgaagggc gggggcggcg gtgcggaacc gaacaacttg atcttcatca acttatcaaa
     1261 aaatgactga aattttgtgg aatcgatacc catgatcttt ttcacctcca tggcgagcgt
     1321 ataggtacaa ctgaggggag aaacctgggg tggacagact tgtactccaa gttctcatgc
     1381 agtacaagtg aaaaatgcgt tacgttcgtt tcatctactt tctctttttc gcatctattt
     1441 cgcctttttc atttcgtcca gcgcccaagt ccacggaatc ccgcgatcta cggaggatgc
     1501 cccgcccgaa tgcctgaccg tcggatttgg attagagcgg cccctacagg ggaaaggatc
     1561 tggacttagt cggcggcgta gcggcggaag aaggagtcgg gcttccagcg gggaggtgcg
     1621 ggagtgttgg cggtgtcgag gagcaggcgg cgagtgaatt cctcataaag agcggcggac
     1681 tggaaatcga cgggttggtt cacgtcatcg gagggagcgt ggtaacggtt ggtgagccag
     1741 tccttgaaga ctttttgctc gggcgtgccg agttcgaagc cgacgtcaac tttgacggag
     1801 ggaacgcctt tcttgatgaa gctgtactga tcgctgcgga tgaaggcgtt acggagaggt
     1861 tcgggatcgg gaatgggctt taggcccatg gactgagcga tggccgcagc gcgcgtgcct
     1921 aggtcggatt cctcaatgcc ttcaatcttg aggatcttca gcgggacgat gggaagaaac
     1981 atgtccacgt tgacgtcggc gatgatggat tttggcgcga cagtaggatg ggcggcgaaa
     2041 tatttggagc cgagaaggcc cttttcttag gcggtgacga gaaggaaaag aatcgagcgc
     2101 tgtagtttct cgggatgagc tttcaggctc gcggcgatat ccataactag cgcgctgccg
     2161 gagccgtcgt ccatcgcgcc gttgtagatg cgatcgccat tgatgggcgc gccgatgccc
     2221 acatggtcga tgtgcgccga gaggacgacg aattcgtttt tgagagcggg atcagagccg
     2281 gtgagtttgg cgacgacatt ggaggattcc agagtctggg tgaggattac agcgttggct
     2341 ttgagtgaaa cggccaaggg gaaatgcgga agcggcttgc ggtcttttcc cagggcagcg
     2401 atttccgcga aggtgtggcc cgaaccggcg aagagttttt cagcggaggc aggattgaag
     2461 gctacgccca attgaaggcc gggagtttca ttgaactcgg catcggcgag atccatggac
     2521 ggatgattgc gattgacgga gatgcgggac cagggaatgt ccatggaggc aggattaaaa
     2581 atggtgatga tgccgatggc gcctgccgcg cgcaacgctt tccagcgctc gccgggggtt
     2641 tgagagtgtg aggctagagc ggtgggaatg cccgagggag aaccggcaag gaagacgacg
     2701 attttgcctt tcaggtcgag gccggcgagt tcatcgagat ttctttccgg gatttgcagg
     2761 ccgtagccga tgaaaactag aggcgaggag aggctggcgg aagcgcgggt ggcacgcgtg
     2821 ttgatgtacg catcgtcggc gaaggagaga gctatcacct ggccgttggt gaggagagtg
     2881 agagaggatt tggattcgtc gacctggtat tggatgaaat ggacggcctg gaagaagccg
     2941 ttcgaaccgg caggctcgag gccggctttc tggagttgct cgacggcgta ggcctgcgct
     3001 ttgcgaagac ctgcgctgcc ggtgtcgcgg ccttcgaggg aatcgtcggc aagaaatttg
     3061 acgtgggacc accaggagtt gccatcgaag tgggaggcgg gcacattttg ctgggctgag
     3121 gaagagacgg ccaaggcgag gaaaagaatc acagactgag tgttgcgcat gagttcaccc
     3181 cggaagcgaa atgaaaggct agaggatagc agggacgaag cggaagagtc acgcgagtgc
     3241 gccatggaac tggttcgact ccccgccgct attcctcagg gcgcagtgac gatcggcagc
     3301 tcgacgaaag ttgcctgccc ggggacatga tagacgcgct gggtggcttt gcgatagtcg
     3361 ccgggcttgg cccagaaaat gttggggacg aaagtctggg ggttgcggtc gtacagcggg
     3421 aaccaactcg actggatttg caccatgatg cggtggccgg gcaggaacac gtgattcgcc
     3481 gtgggaagcg cgaagcgata gagcagcggc ttgtccggcg cgagggcctt gggcgtttca
     3541 aaactttcgc ggtagcggcc gcggaaaata tccgcggcaa ccatgagctg atagccgccc
     3601 atttcgggtt gggcgcccac ttcgtcgggg tacaggtcga tgagtttgac aacccagtcg
     3661 gagtcagtgc cgctggtgga agcgatcagg ttggcgatcg gttgcccgct gatttttacg
     3721 gaagcgctga gcgtatcgga gacgaacacc gcgacatcgg ggcggccgga cgcttcgcgt
     3781 tgatcgtcca caagccactg agaccacgtc aagccattgg agtagccaac gggttgactc
     3841 gggcgggcgc ggaacggcac gggcttggcg gggtcggaga cgtattcgtc gaaaggcgct
     3901 tcgctcgctg cgggtgcggt gaagctgagc ttcagacccg cgttgagata gagcggtgtt
     3961 ggttttacgg cacagccgct ggcgcagccg gagggccacg caggcaaccg ccgccagata
     4021 ttcgtgccgg tttcaaacgc ggcgaccggc gcgacgtcgc tcttcggcgc atcgtctttc
     4081 agatagtgcg ccaggaaggg cgcgagaatt tctctgcgaa aatacaaacc cgtatcgctt
     4141 ccgaatttca gcgcgccaag cgaaccgcca tcaccgatct cctgcccgtg atgccaaggg
     4201 cccatcacca gaaatacttt gtcgttgccg gtgtccttcg gcttgagcac tttgtagacg
     4261 gccatcgccc catagatgtc ttcctgatcc cacaggctgg ataccagcat ggtcggaact
     4321 ttgaggggct gcgtggcgag aatcttgtcc atggcttgct gctgccagaa agcgtcgtag
     4381 gccggatgat cgagaagctt gcgccagaag ccgatttgtt caagaccgtg acggcggccg
     4441 agttcgccgg ccgaactggc ttgcagatac atgtcatagt cgtcgtagtg gctgttccac
     4501 cagtgctcgt cgttttttcg cgtggcttcc tgctcgtaaa tgtagggcat gttcagttcg
     4561 cgaaacgcgc cgtagtgaaa ccagtcgtca cccatccagc cgtcgaccat ggggttcatt
     4621 ggcaccgaga ctttgagcgc gggatgcgga ttgatgagcg ccatgagagg cgtgaagccg
     4681 tcgtacgaaa taccgaggat gccgaccttg ccgttgctct cggggatgtt tttcaccagc
     4741 cagtcgatgg tgtcgtaggt gtcggtggcg tgatcgacca tcgtgggatt gagcgggccg
     4801 tggagcgggc ggttcatcac gtagtcgccc tcggagccgt atttgccacg gatgtcctgg
     4861 acgacgcgga tgtaaccgcc ttcgaggatc acttcgagag cgttgtcgta gccatggagg
     4921 ctggggccga gatgcgagct gtgcgagtgg gtggtgagat cggtggcgtc gtaaggcgtg
     4981 cgggtgagaa gaattggcgc gttcctggcg cctttgggca cgaggatgac ggtgtgcaac
     5041 ttcacgccgt cgcgcatggg aatcatgacg tcgcgccgcg cgtagtcgaa gctatcggtg
     5101 accggcgtga actgggccgg cgtttcgctt ggcaaagtga ggggagcgga ggccggcgga
     5161 gcctgggccg cggaggaatc ggcaatcaag agaacgccga gcattggtgc gagcaacgtt
     5221 agcgtgcgaa cgcagtgaat cggcttcatc gaagtccctc aatgatgcgt ggaatgaggg
     5281 tggattataa cccggattgc ttcgctcgcc agattcagag aatgagcgga gaggttgcga
     5341 agtggctgaa aactcttccg acacggtcaa cttaacggtg tttactggaa cgctttgcgg
     5401 gagtaaaatg gggtgaattg attacgttgt atgcctcatg caaccgttag actgaattcc
     5461 agttccagag acttcccgct aggagcgatc aagatggcga ccgctactct tacgaagccc
     5521 gcagcaaagg gaggcagctt cctcctggaa tcgccgcagc cgggcgacgt tttcacaccc
     5581 gccgatttga cggacgatca gagattgatc gggcagacgg cggaggaatt tgtcctcaag
     5641 gaagttttcc ctttcatcaa ggaactcgaa aacaagaagc ccgggctgat ggccgagctg
     5701 gtgaaaaaag gcggcgaagt cggtttgatg ggcggcggag tgccggagga atacggcggc
     5761 gcgggcctgg acaagatttc caccacggtg ctgaccgaga aactttccat ctatggcggc
     5821 tatgcggtga cccacggagc gcacgccggc atcggcacgc tgccgatcgt ttacttcggc
     5881 acggaagcgc agaagaaaaa atatttgccg aagctggcga cgggtgagtg gatcggcgcg
     5941 tactgtcttt ccgagccgca agccgggtcc gacgcgcaga attcgctgac gcgcgcggaa
     6001 ctcaacgccg aaggcacgca ctacgtcctc aacgggcaga agatgtggat caccaacggc
     6061 ggcttcgccg acgtttacat tgtgttcgcg aaaattgacg gcgaaaaatt cacggccttt
     6121 atcgtcgagc gcaccttccc cggcttcaag cccggcaacg aagagcacaa gatgggcatc
     6181 cacggcagtt cgacaacgcc gatcttcctg gaaaactgca aagtgccgaa ggaaaacctg
     6241 ctgcacgaaa tcgggcgcgg gcacatcgtg gcgtttaaca ttttgaatgc cgggcgcttc
     6301 acgctcggcg cttcctgcgt gggcggctcg aagcacgtgc tcatgacgtc ctcgaaatat
     6361 tcgaaggaac gcaaagcgtt tgggaaacag atcggcgact tcggcctgat gaaggaaaag
     6421 ctggccgaga tggccataca gattttcgcc gtggaatcga tggtgtaccg ctccgcggga
     6481 aacatcgagg cggcaatggc ggcggcttcc ggctccgggg ataaaattca gaacaccatg
     6541 aaagttctgg aggaatacgc catcgagagt tccatcgcca aggtctacgg cagcgagatg
     6601 ctcgatttcg tggtggacga aggcgtgcaa atcttcggcg gatatggttt ccacgaggac
     6661 tatcccgtgt gccgcgcgta tcgcgattcg cgcatcaacc gcatctttga aggaaccaac
     6721 gaaatcaatc gcatgctgat catccagatg ctgatgaagc gcgcgatggg cgggcagctg
     6781 ccgctcattc ccgcggccat gaagctggcg gatgaaatcc tcgcggggcc gtccttcgaa
     6841 gaagcgcctg aaggcgttct cgccgccgaa acccgcgttg tcgccaacac caagaaaatg
     6901 tttctgcaag cggccggagg cgctgtccag aaattccgcg aaaagctcgc cgacgagcag
     6961 gaactgatcg gcgcgctctc gaacgtggtc atggaaatct acgcgatgga gtcctgcctg
     7021 ctgcgcgcgc aaaaagccgc cgcggcaaag ggcgaatccg caacgcagac catgatcgat
     7081 gccgcgcgcg tgttcatcca tgacgccgcc gagcgcgtcg agcacgaagc caagcgcgcc
     7141 atcgccgcca tccacgaagg cgacatgctc accacgcaga tggccgtgct aaagcgtttc
     7201 gcgaagcgtg cgccggtgga taccattgcg ctgcgccggc gcgtcgccgc ggcggtacag
     7261 tcccaggatc gctatccgtt ggagggccgc taggctcgaa cagagctgaa gtgcgaactg
     7321 aatcacttct ccgatcgtcc cttcgtaccg tcccgtatca ggcagggcac gccttccgag
     7381 ggtttgtgta aaggccctcg ggattccccc tagaataaat tcaggcaaaa attcagttcg
     7441 aaacgcgaga gtacgacaag gaatgccccg aaagcccctg atccttgcag ccgtcattgt
     7501 cgcgctgcat ctttgcgaag cagcgacgct aggcaccact gcggccggat ctttgctggc
     7561 aaatctcctg gaaatcttcg cttgcggttt tgccgcagtc atggcttttg gggcgtctcg
     7621 ccgcgggcgc ggcttgagcc gccccttctg gctgatcttc ggcgcgggta tcgcgatgtg
     7681 gggtgtagcg aacctaggct ggatgtacta cgaagtggtg ctgcacaccg aaccgcccac
     7741 aacctccgcg gtacgattct tattcggttt ggaaagcgtg ctgatcgcca tggccttgtt
     7801 cctggatcag gacaaggatt ctccgcggat cgacgcagaa tccgcccttg acttcatcca
     7861 gatcggaatc gtcttctttt ttatttacgt cgaattttac tacctgccgg ctcaccgact
     7921 tgacaactac tccgcattcc tgcgcgagat gcgcgtcgag aacgtggaag atgccttgct
     7981 caccgtgctg gctgctttcc aggcgctgcg cgctcggaaa cagcacattc ggaaacttta
     8041 cggcggcctc gctatttaca tgctgctctt atctgtgtgc gcggcgctgg cccagtatct
     8101 gcaaagtatc aagccagccc caactgggac gttgcgggac ctgctatgga cgttgccgtt
     8161 tctcgttgtt gcaatgtggg cagcgcagtg gcagccgagt cctgcggcgg aaactggttc
     8221 acgattgcgg cggaagacgt tcggcgaatt gatgctgacc aacgccacat ttgtgctggc
     8281 gccattgatc atactttggc aggtctcgca actggaagcg gagtggcgcc tgcttcgttt
     8341 ctcgcttctt ggaatttcga tcgtgtgctt tgccgcgcgg ctgggaatca gccagtatcg
     8401 cgaggccaaa agctcaaatg cggtgatgac acacacactg gcgatggatt ccgccgtgaa
     8461 tggcatggcc attctcgatg cccgcgggaa atacatatat gtcaacccgg cctacgcacg
     8521 gatgatcggg aataccaatc gggaagcgct gctgggcaag tcctggcgag aggtttccca
     8581 cggacggacc gtaacgccgg tggagtcgga gattcgtaag gccctgatgc agcatggtaa
     8641 gtggttcggc ccgctcaccg tccttcacag cgacggaacg gtggtcccca tggaaatggc
     8701 cattacctcc ctgcccgaag gcggcacgat ttgcgtgagc cgcgacatca cccagcgggt
     8761 gagcgcgcag cgcgcccgcg ccgaaaccga aattaaatat cgcatgctcg tcgagcaggt
     8821 cgccgccgta agctacatcg cggaactcgg ggtcaatggc caatggctct acgtcagccc
     8881 acaggtcgaa acgatgttcg gcttttccgc ggaggaatgg ctttcgaatt cgagagattg
     8941 gatacgtcac atccctatcg aagaccaccc catcgtgaat gccgcggagg aagccagcgg
     9001 acgcggcgaa cccttccagg ccgaatatcg catcacgcgc aaggacggaa cgaccatatg
     9061 ggtgagcgac accgcggtcg tggtgcgcgg cagtgacagt catcccgtca tggaaggcct
     9121 gatcgtcgat atcaccgacc ggaaaatgct cgaaaatcaa ttgctccagg cgcgcaagat
     9181 ggaggccgtg gggcggctgg cgggaggcgt cgcacacgat ttcaataatc ttctgacgat
     9241 catcaagggt tacgtcgaga tggccctgca gcgatgcctg gaccggccgg agttgcacgg
     9301 cgacatccgg cgcatcgatg acgctgccga tcgcgcagtc acgctggttc ggcagttgct
     9361 ggccttcagc cgcaagcagg ttctgcggcc gaaaatcctc gatctcaacg ccatcgttgt
     9421 gaatctcgac cagctcctcc gccggctgat gaacgaaaac atcgagatga aaacatttgt
     9481 gtcgaaagac gttggagcga tcaaggccga tcccg
//
LOCUS       DASYVO010000078        18266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_226932, whole genome shotgun sequence.
ACCESSION   DASYVO010000078 DASYVO010000000
VERSION     DASYVO010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 18266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18266
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226932"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VGR03_02510"
     CDS             complement(<1..400)
                     /locus_tag="VGR03_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993180.1"
                     /translation="MHGKPVETILAYFFGVPAAAGIALSYLQPYLSRAKLWLSAKDEG
                     QRQQVRGIDIPRRSEPNLVFQTPQIGGVGLENNVWSHGGSPNAQRYLALMVPIKNEML
                     PGKDVGRGEGIKAEIILSTGGTVLREWSPAR"
     gene            644..1702
                     /locus_tag="VGR03_02515"
     CDS             644..1702
                     /locus_tag="VGR03_02515"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV1993181.1"
                     /translation="MKTIKGPGIFLAQFAGDAAPFNSLKSIAAWAAKLGYKGVQIPCW
                     DARLFDLQRAAESKTYCDEIRGTVAGHGLEITELSTHLQGQLVAVHPAYDLAFDGFAP
                     EKLRGNPRARRAWAVEQLLLAARASANLGLAAHVTFSGALAWPYLYPWPQRPAGLIET
                     AFDELAARWRPLLDAFDKAGVDLCFEIHPGEDLHDGVTFEMFLERCGNHPRCNVLYDP
                     SHFVLQQLDYLEFIDLYRERIKMFHVKDAEFRPNGRQGVYGGYQPWINRAGRFRSPGD
                     GQVDFGAIFSKLTQYNFPGWAVLEWECCIKSNEQGAAEGAPFIQRHIIQAAGSTFDDF
                     ASSGADQRGNRRMLGLEG"
     gene            1873..3060
                     /locus_tag="VGR03_02520"
     CDS             1873..3060
                     /locus_tag="VGR03_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1993182.1"
                     /translation="MTADSKANDAKNRRLRLGMVGGGPGAFIGAVHRIAARMDDRFEL
                     VAAALSSDPAKSRAAALELHIAPERAYGSFAEMAAGEAKRPDRIDAVSIVTPNHVHFG
                     PAKAFLEAGIHVICDKPLTTTVEDALALAEIVRRSGLIFGLTHNYTGYPVVRQAREMI
                     EAGELGRIRVVQVEYAQDWLTTAVEATGHKQAVWRTDPAQSGPAGSLGDIGTHAYNIA
                     CFVTGLRCEQVAADVSIFVPGRRLDDNVQVLLRFEGGTRGMLWASQVATGNENNLRLR
                     VYGEKAGLEWGQENPNYLRFTPYGKPPLTISRAGAGATASAKHASRIPSGHPEGYLEA
                     FAQLYADLAEQIAARNEGRAPMPASLLVPGVEEGVAGVQFIDGVLASSRKNSAWVNLP
                     SSR"
     gene            3322..4575
                     /locus_tag="VGR03_02525"
     CDS             3322..4575
                     /locus_tag="VGR03_02525"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEV1993183.1"
                     /translation="MDIQRPSNARAKKIRRIVYGTATLLLLGGVTYGLSKLRPAAPSV
                     DRATIWPDEVKRGPMLREVRGLGTLVPEDIRWIPAQTDSRVDRIVLRPGAVVKPDSII
                     LELSDLQLQRDALDAEYQLRAAEADYANLKVQVNSELMNQKAAAAGVRSEYEQSKIQH
                     EVDEKLHEEGIGSDVTRKLSKVKEEQLAIRVQLEDERTQIAGESSKARLAAQQARVDQ
                     QKVLYQLRRSQVDALHVRAGISGVLQLVPVEVGQRVTPGLNIARVADPKKLKAEIKIA
                     ETQAKDVTIGQKAAIDTRNGVVNGHVSRIDPSVLNGTVTVDVAITDPLPNGARPDLSV
                     DGTVELENLKDVLYVGRPVHGQADSTIGIFKIIDDGSEAVRVNVKLGRSSVNTIEIVQ
                     GLKVGDKVILSDMSAWDNFDRVRLK"
     gene            4599..7109
                     /locus_tag="VGR03_02530"
     CDS             4599..7109
                     /locus_tag="VGR03_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1993184.1"
                     /translation="MHTLLQDVRYGLRMLAKNPGFTVIAILTLALGIGANTAIFSLLN
                     QVLLRRLPVRNPQELVVLKSPGPKRGHVWSDGDDAEVFSYPLYKGLAKNTAVFNGLLA
                     RFQFAASIASHGQTDRGSGELVTGNYFEVLGVRPALGRVLSPADDDVQGAHPVLVLSH
                     AYWTRHFGGDAGALNQTILVNNTEMTIVGVAQAGFTGIQVGQTPDIFVPMTMKAQMTP
                     IRNGLDDWNDSFLAVLARRKPGVSMEQAQAGINVDYPGLLEQQAATLKMRADGKDRKE
                     FLSKKIVLSPGAQGRTTAQRDSGPALKALFAMVALVLLIACTNVANLLLAKAAARQRE
                     FAIRSALGATPGRMMRQLLVESFLCALGGGVLGLILGAWIMNILTQAVVSEAMVQGIT
                     ADVDGSVLGFAAAATVVSALLFGLIPAWRATRTGVSQMLKDQGSTTSAGPGHVRFRKY
                     LVAGQVAFTLLLLTGGAMFSRTLWNLRKQNLGLSTENLITFSISPQLSGYNDARTVAL
                     VDQLRERLGALPGVLGVGSSQIPVLTGTDMGANITVEGRQNLDTDDRHVNFDAVSPNY
                     FSTMRIPLLSGREFNARDTAASTKVAIINEAMAKEFFAKRNPIGVHFALGSGNDVKPN
                     IEIVGVVKNAIVEHVRDGELPYFYQPYSQFGKLFGMSFYIRSQQEPLLIANMLRETVR
                     GADGNLPVYDLKTVQRVVDEDLFAERVIAGLSAAFGGLAALLAALGIYGVLAYLVVQR
                     TREIGIRVALGAGSGHVRGLVFKEVGWMVMAGALVGLPLAYGLARLGESLLYGVHAGD
                     VPVYAASLGIICLMAFAACYIPSRRATRIDPIVALRYE"
     gene            7194..7904
                     /locus_tag="VGR03_02535"
     CDS             7194..7904
                     /locus_tag="VGR03_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1993185.1"
                     /translation="MTDATATLIKMEGVKKVFYTDEVETHALSEIHLEIKKGQYISIA
                     GPSGCGKSTLLSILGLLDSPTGGEYTLNGQPVAKLTISERTRIRNREIGFIFQAFNLI
                     GDLTVYENVELPLTYRGTSAAERKQKVQHALERVGMAHRLRHYPSQLSGGQQQRVAVA
                     RALVGDPLILMADEPTGNLDSKNGEAVMELLRDLHRNGATVCMVTHDQRYANFAERTI
                     HLFDGQIVEETQEARVGA"
     gene            7921..10365
                     /locus_tag="VGR03_02540"
     CDS             7921..10365
                     /locus_tag="VGR03_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1993186.1"
                     /translation="MSTIWQDLRYAIRVLAKSPGFTAVAVLTLALGIGANTAIFSILY
                     GVLLRPLPFHEPDHLYGVTLNLKGELSETSFTFHQFEYIKQHSQWYEGMAASTPVGFN
                     LSAEGTAQRVTALHITSDYFKVLGAQPYLGRDFQAEDDRAGSAPTAILSHHLWQQHFG
                     RETSIVGKTIRLDGEPYLVIGVMPAGYGNNQSGFVPSEFAQFQHVDLWAMMAPVAKTI
                     GSGENLDVIARLKPGVTPEQARAQIDALSDSFRKDQLEGRARAQNLALYSLQKVIGGE
                     VSSYLWILLGAVSFVLLIACANVANLLLARGAGRLQEVAVRTAMGATRGRLIRQFLTE
                     SLLLALAGAGAGLLLAQVSFNSLLALAPVQLPRLNEIHVDLWAFLFAVSIAVSGGILF
                     GMAPAIQAARTDVHSMLKIGTSRSTAGSWSGRMRGALVAAEIALSLILLVGAGLLGET
                     LLNLLRVPAGIDPKGVLSAEIWLTGSRYRSTAELATFFETVANRLKSIPGVQNATVVA
                     SGQPLERGGNIPVKVNGAFWGSVEYRVVTPEYFRTLHVAMLQGHEFTAADTETSQPIA
                     IVNQAFVRDVLSFKEPLGASVKAGNSDPARVIVGVAADVRSHLDAPAGPAVFVPAAQA
                     NFSITLLFDQWFPTHVLVRTSGDPLLPEKTLAGVIQETDPSIPVGKIRTMDEILARSL
                     SYQRFMLTLVSVFAGLAMILAAIGIYGVMSYTVVQRTRELGVRTALGATPRSILLLVL
                     GEGSKFAMIGTVIGIIGAVALHRTLQSLLFGVRATSVLTLGAAAAFLFAVALLACYLP
                     ARRATRVDPLVALRYE"
     gene            10376..12814
                     /locus_tag="VGR03_02545"
     CDS             10376..12814
                     /locus_tag="VGR03_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1993187.1"
                     /translation="MHTLWQDIRYGARMLWKHRLATIVCATALALGIGANTAMFSVAE
                     AFLVRSVPFENPDRIVAMVNVRPQQNIDRNAVAPATYLDWQTQAKSFEQLGAYEYAEV
                     NLTGNREPEKVQAFEVTANFFGLLGVQPKMGRTFLPEEEQPGKDQEIILSYGLWERRY
                     ASDPNILNKIVKVDGKSFTIVGVMDKGFDFPITAEAWMPLALTVKQQSERNSRYIWVL
                     GKLKPDTSIKQAVAEMSGLAQRESDAYPDSYKGWQLRVMPVREFTTSYLTRQYILLLM
                     GAVGFVLLIACADVANVQFARVSGRQKELAVRAAMGASRTRIVCQLLTESVLLALLGS
                     VAGLLIAQWDLVLILAHMPADVAKYVAGWKTISLDSGAFLFTLAIAVFSGILSGIAPA
                     LMTSRANVSETLKESGRGSSAGRARHRLRNALVVAEISLSLVLLVGAGLLVKNFTALV
                     SVNERYRPESLLTLNLHLPELQYNTRSGRVTFHEQVLQRLSTIPNVQSVAMVTHVPYS
                     DGGGVGTRTFSIEGRPATDRGEVRNAIVQTISPSYLDLMQIALREGRELTESDADSTL
                     PVAVISKSLARRYFPDEKPLGRKLKVGQADDSSPWLTIVGIVDDVHYSWIEKDDVPTI
                     YRSYRQSSPFYASLVLRTGGDPLSLVSSVRGQIAAVDPDLSLYNIKPLSVVISDSIVG
                     IAYVAVMMGILGIIALVLASVGVYGVMSYTVSERTNEIGIRMAMGATASDIQRLVLGK
                     GALLTLIGMGIGLPVAFVLATFLSSLLFGVKAADPVAFIVLPLVLAAVATLASYLPAR
                     RAVRVDPITALRYE"
     gene            12888..13604
                     /locus_tag="VGR03_02550"
     CDS             12888..13604
                     /locus_tag="VGR03_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1993188.1"
                     /translation="MEPLIQLRNMEKVVEAGPNKFYLLRRINLDIQPGEFVTIMGPSG
                     AGKSTLLSIIGMLDGAWSGDYYFHGEPVHKLNIKKRNELHKANIGFVFQSYHLIDNLT
                     VFENLEVPLSYKNVPHKERVSLVCDALDRFHIVGKKDLFPNQLSGGQQQLVGVARAVI
                     LSPKLILADEPTGNLHTAQGEEIMQLFKRLNDAGTTIIQVTHSERNAAHGSRIVQLKD
                     GWVVGDNATAGAQVGAAQPA"
     gene            13732..15072
                     /locus_tag="VGR03_02555"
     CDS             13732..15072
                     /locus_tag="VGR03_02555"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV1993189.1"
                     /translation="MADDQADIRDALRLLLKREGYETQALASPAEALSAIESREFDAV
                     LMDLNYARDTTSGQEGLDLLTRIQMLDSTLPVIVMTAWSSIEVAVEAMRRGARDFVQK
                     PWENTRLLAILRTQIDLRQALRQASRLEAENRILRAESRPTFLAKSPAMVPVLELIGQ
                     IGPSDANVLITGEHGTGKEVVARTLHALSARATKPMVTVNAGGLSEGIFESELFGHVK
                     GAFTDARSDRVGRFELADGSTLFLDEIANVPLNLQAKLLRVLETGDLERVGSSTTRRV
                     NARVLAATNADVNAEAAAGRFRQDLLFRLNTIEIHIPPLRDRREDIDLLAHHFLHGYS
                     QRYRKSISGFDSAAVKALHEHSWPGNVRELDHAVERGVLLSPGPSIRAADLGLRAQAA
                     GGAAKLEEMSLDEVERYLIQRTLSRHEGNVSQAAKALGLSRSAMYRRLQKHGLE"
     gene            15069..16406
                     /locus_tag="VGR03_02560"
     CDS             15069..16406
                     /locus_tag="VGR03_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1993190.1"
                     /translation="MTRLTYERRIQLLALAAGFPGSLIAMILLWHGNYSSGAVWTLAF
                     FIFSLWLGFAFSLRHRVVFSLQTLSNLLAALREEDFSLRARGARSDDAMGEVMIEVNA
                     LSETLRQQRLGALEATALLRTVMEEIDLAIFTFDNSTKLRLVNRAGERLMGRPEGRLL
                     GFTAEELGLGACLEGEAAHTMELAFPGGSGRWGLRRGTFRQGGLPHHLVVLSDLSRTL
                     RDEERKAWQRLIRVLGHELNNSLAPIQSVAQGLESGLLTASKSSELQSEASVSILDDL
                     RQGLGIIRSRTEALGRFMAAYAQLARLPQPKLLPVNVSEWIGRTIKLETRVKVSLLKG
                     PDVVVSADADQLEQLLINLVRNAADATLETGGSVQVGWSRQGAQLDVWVIDEGPGLPN
                     TSNLFVPFFTTKQGGSGIGLVLSRQIAEAHGGELTLKNRSDTRGCEARLRLPI"
     gene            16804..>18266
                     /gene="kdpA"
                     /locus_tag="VGR03_02565"
     CDS             16804..>18266
                     /gene="kdpA"
                     /locus_tag="VGR03_02565"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEV1993191.1"
                     /translation="MTANGWFQIFLVLGVIFAVTKPLGVFMTRVFNREKTFLDPVLRP
                     IEKLVYRLTGVDEKHEMRWTEYAVAMLLFSGVSMALLYLIERTQLWLPLNPQKLANVP
                     QALAFNTAASFTTNTNWQNYSGEAAMSYLTQMAGLAYHNFTSAAAGIVMAIVVIRGIA
                     RKETDKLGNFWVDMTRCLLWVLLPVCLVGSLTLVSQGVVQNLKPYTTVELTEPQTVQV
                     TGADGKTSTQVVSQQVIAQGPVASQEIIKELGTNGGGFFNANSAHPFENPTPLSNFLE
                     LVLIFAISSGLTYTLGRMTGSPRHGWAVWAAMVVLFLMGVTTAYWAEARGNPILTGVD
                     QRAGPLQPGGNMEGKEVRFGIANSALFATMTTDTSCGAVNSMHDSFTPLGGMVPLINI
                     MLGEVVFGGVGSGLYGVVVFVILAVFIAGLMVGRTPEYLGKKIESYDVKMAMLSILIM
                     TFVILGFSAIAIVQPFGTSSISNPGPHGLSQVLYAYTS"
BASE COUNT         3726 a         4956 c         5733 g         3851 t
ORIGIN      
        1 acctagctgg agaccattca cgtagcaccg tcccgcccgt tgaaagaatt atctctgcct
       61 tgatcccttc tcctctcccc acgtccttcc cgggtaacat ctcgttctta atcggcacca
      121 tcaacgcaag ataacgctga gcattgggac tacctccatg tgaccaaacg ttgttttcta
      181 aacctacgcc tccgatttgt ggagtttgaa atactaggtt gggttcactg cgtcgcggga
      241 tatctatccc cctaacttgt tgtctttggc cctcgtcctt agctgatagc caaagtttcg
      301 cgcgcgaaag atagggctgc agatagctca gagcgatacc agccgccgcc ggaactccaa
      361 agaagtaagc aagtatcgtc tctacaggct tgccatgcat ctacgctgcc aagcccgcga
      421 tgactccgga cagagccgaa aaaatccaaa ctaagaggtg aatgtcggca agggttttcc
      481 atagagttga ttcgcgaaat ctggcgagca cgtagatcta cctcttttcc cattctaaat
      541 gggaagcgtg aggcgtggcg cagagatttc ttttgaggtg gaaaatgagt agagtagcgt
      601 ctcgcgctga aaccatcgta catgcggggg agtcgaggag acgatgaaaa caatcaaggg
      661 accggggatt tttctggcgc agtttgcggg ggatgcagcg ccgttcaaca gtttgaagag
      721 cattgctgcg tgggcggcca aacttgggta caagggcgtg cagattccgt gctgggatgc
      781 gcggctgttt gatttgcaga gagcggcaga gagcaagacg tattgtgacg agatccgggg
      841 gacggtggcg ggccatggtc tggagatcac cgaactttcg acgcatcttc aggggcagtt
      901 ggtggcggtg catcccgcgt atgacctggc gttcgatggg tttgcgccgg agaaactgag
      961 gggcaatccg cgagcgaggc gggcatgggc ggttgagcag ttattgcttg cggcgcgcgc
     1021 gtccgccaat ctgggacttg cggcacatgt gacattttcc ggggcgctgg cgtggccgta
     1081 cttgtatcct tggccgcaga ggccggccgg gctgattgaa actgcttttg acgagctggc
     1141 ggcgcgctgg cgtcccctct tggatgcgtt cgataaagcg ggagtagatc tgtgcttcga
     1201 gatccatccg ggcgaagatt tgcatgatgg ggtcacgttc gaaatgtttt tggagcgctg
     1261 tggcaatcat ccgcgatgca acgtgcttta tgacccgagt catttcgtgc tgcagcagct
     1321 tgattatctg gaattcatcg atctgtaccg cgaacgcatc aagatgttcc acgtgaagga
     1381 tgcggagttt cgtccaaatg gccgacaggg cgtttacggg ggctatcagc cgtggatcaa
     1441 tcgggcgggg aggtttcgtt cgccgggaga tgggcaagtg gacttcggcg cgatattttc
     1501 caagctgacg cagtataact ttccgggatg ggcggtattg gagtgggagt gttgcatcaa
     1561 gagcaacgag cagggagccg cggaaggcgc gccgtttatt cagaggcata tcattcaggc
     1621 ggcgggcagc acgtttgatg acttcgcaag ttcaggcgcg gatcaacggg gcaatcggcg
     1681 gatgctggga ctcgaagggt gatgaagaat tggagtaaag aagcgaagcg cattcgatcg
     1741 tgaaaagcag cccgacgtca aattgattga aggggttgaa gttgagattc ttcgggcatc
     1801 cttatcggat gccctcagaa tgacaccgct gtctttttga gcaggatgct agattgagat
     1861 gggatgaggg acatgacagc agattcaaaa gcgaatgatg caaaaaaccg gcgtctccgg
     1921 ctgggtatgg tcggtggcgg accgggcgcg ttcattgggg cagtgcaccg cattgcggcg
     1981 cggatggacg atcgttttga gctggtggcg gcggcattgt cttccgatcc cgcgaaaagc
     2041 cgtgcggcgg ctctggagct gcatatcgct ccggagcgcg cttacggaag tttcgcggaa
     2101 atggcggcgg gggaagcgaa gaggccggac cggattgacg cggtctcgat cgtcacgccc
     2161 aatcacgttc atttcgggcc ggcgaaagcg tttttggagg cgggcattca cgtcatctgc
     2221 gataagccgc tgaccacgac ggtggaagat gcgctggcgc tggcggagat cgttcgccgc
     2281 agcgggctaa tctttggctt gacgcacaat tacacgggct atcccgtggt gcggcaagcg
     2341 cgggagatga tcgaggccgg ggagctgggg cgaattcgcg tggtgcaagt ggagtacgcg
     2401 caggactggc tgaccacagc ggtggaggcg acgggacaca aacaggcggt ttggcggacc
     2461 gatcccgcac agagcggacc cgcgggcagc ctgggagaca ttggaacgca tgcgtacaac
     2521 atcgcgtgtt ttgtgaccgg actgcgctgc gagcaggtgg cggcagacgt ttccattttc
     2581 gtgccggggc ggcggctcga cgacaacgta caagtgctct tgcgatttga gggcggcacg
     2641 cgcggaatgt tgtgggcgag ccaggtggct acgggaaacg agaacaatct gcgcttgcgg
     2701 gtgtatggag agaaggcggg gctcgagtgg gggcaggaga atccgaacta tctgcggttt
     2761 acgccgtacg gcaagccgcc gcttacgatc agccgggccg gggccggtgc gacggcttcg
     2821 gcgaagcatg cgagccgcat cccgtccggg catccggagg ggtacctgga ggcgttcgcg
     2881 cagctttatg cggatttggc ggagcagatt gccgcgcgga acgaggggcg cgctccgatg
     2941 ccggcgtcgc tgctggtacc gggggtggag gaaggcgtgg caggggtgca gttcatcgat
     3001 ggagtactag cgtcttctcg gaagaactcg gcgtgggtga atttaccttc ctcccggtaa
     3061 tttgagggga gtgccaggga tgggcgggcg gacggcagag aaaaccaagc cggactggag
     3121 tccggcccac cacgggcaaa ccatgcacga gcgaaggagc ggagtgcttc ccgcttgttc
     3181 gaaggtggga cggctaatcc catttccggt cgatttgggg agattccgag gaaagaattc
     3241 ctgttaactt attgatatta aatgggctta gcggagggaa cctggggccg gagagttgcg
     3301 tttaaagagg catcaatata tatggacatc caacgaccat caaacgctcg cgccaagaaa
     3361 atccgacgga ttgtatacgg caccgccaca ctcctgctgt tgggaggggt aacttatgga
     3421 ctttccaagc tgcggcctgc cgctcccagc gtggaccggg ccaccatctg gcctgatgaa
     3481 gtgaagcgcg gccccatgct gcgggaggta cgtggattgg ggacgcttgt gcccgaagac
     3541 attcgctgga ttcccgcgca gacggattcg cgggtagatc gaattgtcct tcgacccgga
     3601 gcggtagtga agcccgattc gatcattctg gaactcagcg atctgcaatt gcagcgcgat
     3661 gcgctggatg cggaatacca gctgagggca gcggaagcgg actacgccaa cctgaaagtt
     3721 caagtaaaca gcgaactgat gaaccagaaa gcggcggcgg cgggggttcg cagcgagtac
     3781 gaacagtcga agatccaaca cgaagtagac gagaaattgc acgaagaggg catcggctcc
     3841 gatgtgacac ggaagctctc gaaagtgaaa gaggaacagc tcgccattcg agtgcaactt
     3901 gaagatgaac ggacgcagat tgccggggag tcctcgaaag cgcggctggc ggcgcagcag
     3961 gcgcgcgtcg accaacaaaa ggtgctgtac caactccggc gcagccaggt tgatgcgttg
     4021 cacgttcgag cgggaatcag cggcgtgctg cagctcgttc cggtggaagt gggacagcgg
     4081 gtgacaccgg gattgaatat tgcgcgcgtc gcggacccga agaaactgaa agcggaaatc
     4141 aagatcgcgg agacgcaggc gaaagacgta acgattgggc agaaagccgc gattgacacg
     4201 cggaacgggg tggtgaacgg ccacgtttcg cggatcgatc cttccgtcct gaacggcacc
     4261 gtgacggtgg acgtggccat cacggatccc ctgccgaatg gcgcgaggcc ggacttgagc
     4321 gttgatggaa ccgtggagct cgagaatttg aaggacgtgc tttatgtggg ccggccggtc
     4381 cacggccagg cggacagcac gatcggaatt ttcaagatta tcgatgacgg atcggaagcc
     4441 gtgcgcgtga atgttaagtt ggggcggagc tcggtgaaca cgatcgaaat cgtgcaggga
     4501 ttgaaggtcg gggacaaagt gattctctcg gacatgtccg cctgggacaa cttcgaccgg
     4561 gtgcggctga agtagagcgg cgtaggagga ttgaagagat gcacacgctc ttgcaggacg
     4621 tgcgctacgg cctgcggatg ctggccaaga atccagggtt tacggtgatt gcgatcctca
     4681 ccctagcgct gggtatcggc gcgaataccg caattttttc tctgctcaat caggtcctgt
     4741 tgcgccgtct acccgtccgc aatccccaag agttagtggt gctgaagtcg cccggaccga
     4801 aacgtgggca tgtgtggagt gatggcgacg atgcggaggt tttttcctac ccactgtaca
     4861 aggggctggc gaagaacacg gcggttttca atggactgct cgcccgtttt cagttcgcgg
     4921 ctagcatcgc gagtcatggg cagacggacc gtggttcggg cgaactcgta accgggaatt
     4981 atttcgaggt gctgggcgtg cggccggccc tcggtcgagt cctttcaccg gccgatgacg
     5041 atgtgcaggg ggcacatcca gtcctcgtgc tgagccatgc gtactggacg cggcatttcg
     5101 gcggggatgc gggggcgctg aatcagacga ttctcgtgaa caacaccgaa atgactatcg
     5161 tcggcgttgc gcaagcagga ttcaccggca tccaggtggg acagacgccg gacatttttg
     5221 tccccatgac catgaaggcg cagatgacgc cgatccgcaa tgggctggac gactggaacg
     5281 attcgttctt ggcagtgttg gcccggcgga aaccaggcgt ctccatggag caggcgcagg
     5341 cggggatcaa cgttgattac ccggggctgc tcgagcagca agctgcaacc cttaagatgc
     5401 gcgcggacgg aaaagacagg aaggaatttc taagcaagaa gattgtactg tctcccgggg
     5461 cgcaggggag gacgacggct cagagggatt ccgggccagc gctgaaggcg ctgtttgcta
     5521 tggtggcgtt ggtgctgctg attgcgtgca ccaacgtggc gaatctgctg ctggcaaagg
     5581 cggcggcacg gcagcgcgag tttgctatcc gttcggcgct gggtgccacg ccggggcgga
     5641 tgatgcggca actgctggtg gaaagcttcc tttgcgcact gggcggaggg gttctgggac
     5701 tgattttggg cgcctggata atgaacattt tgacgcaagc cgtcgtttct gaagcaatgg
     5761 tacaagggat cacggcagat gtggatggca gtgtattggg gtttgcggcg gcggcaacgg
     5821 ttgtatcggc gttgcttttc gggctgatcc ccgcgtggcg cgcgacgcga accggcgtct
     5881 cgcagatgct gaaggaccaa ggatcaacga catccgcggg cccggggcac gtgcgattcc
     5941 gcaagtacct cgttgcgggg caggtggcgt tcacactgct gttgctgacg ggcggggcca
     6001 tgttttcccg gacgctgtgg aacttgcgga agcaaaacct gggattgagc acggagaacc
     6061 tgatcacatt ttcgatttcg ccgcaattga gcggctacaa cgacgcgagg acggtggccc
     6121 ttgtcgacca gttgagggag cggctggggg cgcttccagg agtgctgggg gtggggtcgt
     6181 cgcagattcc cgtgctgacc ggaaccgata tgggcgcgaa catcacggtg gaaggccgtc
     6241 aaaacctgga tacggatgac cggcacgtga attttgacgc ggtgagtccg aattattttt
     6301 cgaccatgcg gatcccgttg ctttcggggc gcgaattcaa cgccagagac acagcggcga
     6361 gcacgaaagt ggcgatcatc aatgaagcca tggcgaaaga gttctttgcg aagagaaacc
     6421 caatcggggt gcacttcgcg ctgggctcgg ggaacgacgt caagccaaac atagagatcg
     6481 ttggcgtggt gaagaatgcg attgtggaac acgtccggga tggagagctg ccgtatttct
     6541 atcagcccta ttcgcaattc ggaaaattgt tcgggatgag tttctacatc cgcagccaac
     6601 aagaacctct gctgattgca aacatgctgc gggagacggt tcggggagcg gacggcaact
     6661 tgccggtgta cgatctgaag acggtgcagc gcgtggtgga tgaagaccta tttgcagagc
     6721 gcgtgatcgc ggggctttca gcggcatttg gcgggttggc ggcgttgctg gcggcgctgg
     6781 gaatttacgg ggtgctggcg tacctcgtcg tgcagcggac gcgggaaatt ggaattcgcg
     6841 tggcgctggg ggcgggtagc ggacacgtga gagggctggt gttcaaggaa gtcgggtgga
     6901 tggtgatggc gggcgcgctg gtgggattgc cattggcata cggactggcg cggctgggcg
     6961 aatcgctgtt gtacggagtg cacgcaggcg acgttcccgt gtatgcggca tcgctaggga
     7021 tcatctgcct catggctttc gcggcgtgct acatcccatc gcgacgagca acgcggatcg
     7081 atcccatcgt agccctacgc tacgaatagc cacgagtatg attttgtctt gtgcgcacag
     7141 gagcgggtgc aggtgaaacg agttttcgag atcgaatacg agaaggagag caaatgacgg
     7201 acgcaaccgc aacactcatc aagatggaag gcgtaaagaa agttttttat acggatgagg
     7261 tcgagacgca cgctttgtcc gagattcacc tggagatcaa gaaggggcag tacatctcga
     7321 tcgccgggcc ttcgggttgc ggcaaatcga cattgctctc cattctcgga cttctcgatt
     7381 caccaacggg cggggaatat acgctcaacg gacagccggt ggcgaaactt acgatcagcg
     7441 agcgcacgcg aatccgcaac cgcgagattg gatttatttt ccaagcgttc aacctgatcg
     7501 gcgacctgac ggtctatgaa aacgtcgaac tcccgctgac ctaccgcggt accagcgcgg
     7561 cggagaggaa gcagaaagtg cagcacgcgc tcgagcgcgt cggcatggcg caccggctgc
     7621 gtcactaccc ctcgcaactc tccggcggtc agcagcagcg cgttgccgtg gcacgtgcac
     7681 tggttggcga tccgctgatt ctgatggctg atgaacccac gggcaacctc gattcgaaga
     7741 acggcgaagc ggtcatggag ttgctgcgcg acttgcaccg caacggcgcg acggtttgca
     7801 tggtgacaca cgaccagcgc tacgcgaatt tcgccgagcg caccattcac ctgttcgatg
     7861 gccagatcgt cgaggaaaca caggaagcgc gcgtcggagc ataacgccgg gaggtgcagc
     7921 atgagtacca tctggcagga ccttcgctac gcgatccgtg ttctggcaaa gagtccgggg
     7981 ttcaccgccg tcgccgtttt gacgttggcg ctgggtatcg gcgccaacac cgccattttc
     8041 agcatcctat acggcgttct gctccggcca cttccctttc acgaaccgga ccatctctat
     8101 ggcgtgaccc tgaacttaaa gggcgagctg agtgaaacca gcttcacttt tcatcagttt
     8161 gaatatatca agcagcacag ccagtggtat gagggcatgg cagcttccac tcccgtgggg
     8221 ttcaaccttt cagccgaagg aactgcgcag cgtgtcactg ccctccacat aacatccgac
     8281 tatttcaaag tcctcggtgc acaaccatat ctcggccggg attttcaggc cgaagatgac
     8341 cgggctggca gcgcaccgac cgccattttg agccaccacc tgtggcagca acatttcggc
     8401 agagaaacga gcatcgttgg aaagacgatt cgcctggatg gcgagcctta tctcgtgatc
     8461 ggggtgatgc ctgccgggta cggtaacaac cagagcgggt tcgtgccgtc tgaattcgcg
     8521 caattccagc acgtcgacct gtgggcgatg atggctccgg tggcgaaaac catcggcagc
     8581 ggagaaaacc tggatgtgat tgcacgcctg aagccgggtg tcacgcccga gcaggcgcgc
     8641 gcgcagatcg atgcgctgtc ggattccttc cgcaaggatc aactggaagg acgcgcacgg
     8701 gcgcaaaact tggcgctcta ttcgcttcag aaagtcattg ggggcgaagt cagcagttat
     8761 ttgtggattc tgctcggtgc tgtttccttt gtgcttctga ttgcctgcgc gaacgttgcg
     8821 aatctcctgc tggcgcgcgg cgccggacga ttgcaggaag tggccgtgcg gaccgcgatg
     8881 ggggccacgc gcggtcggtt gatccgtcaa ttcctgacag agagcctatt gcttgcactc
     8941 gcgggcgccg gagccggcct gctgctcgcg caggtgagct ttaattccct gctggcgctt
     9001 gctcccgttc agcttccacg cctgaacgag attcacgtcg atctttgggc atttctgttt
     9061 gcggtaagta ttgctgtttc cggaggaatt ctctttggga tggcacccgc gattcaggcg
     9121 gcgcgcaccg acgtccactc catgctgaag atcgggacga gccggtcgac ggcggggagc
     9181 tggagcggaa gaatgcgggg cgcgttggtg gccgcggaga tcgcgctttc cctcattctg
     9241 ctagtaggcg ccggattgct cggggaaacg ttgctcaatc ttctgcgggt cccggcggga
     9301 atcgatccca aaggagttct ttccgcggaa atctggctga ccggctcgcg gtatcgttcg
     9361 acggctgaac tcgcgacttt ttttgaaaca gtcgccaatc gtttgaaatc gattccggga
     9421 gttcaaaatg caacggtggt ggcttcgggc cagccacttg agcggggtgg gaacattccg
     9481 gtgaaggtga acggcgcatt ctggggctcg gtcgagtacc gagtcgtgac gccggaatac
     9541 ttccgcacgt tgcatgtcgc catgcttcaa gggcatgagt tcacggctgc ggacacggaa
     9601 acatcgcaac ccatcgcgat cgtcaatcaa gcgttcgttc gggatgtact ttccttcaag
     9661 gagccactgg gcgcctcggt caaagccggg aacagcgatc cggcacgcgt catcgtgggc
     9721 gtggcagccg acgtgcggtc gcatttggat gcgccggcag gaccggctgt attcgtccca
     9781 gcggcacaag ccaacttctc catcacgctg ctgtttgacc aatggtttcc cactcacgtc
     9841 cttgtgcgta cttcgggcga cccgttgctg ccggagaaga cgcttgccgg ggtgatccag
     9901 gaaactgatc caagcattcc ggtgggaaaa atccgcacga tggacgaaat actggcgcgc
     9961 tccctttcct atcagcgctt catgttgacg ctggtgtcgg tattcgcggg actggcgatg
    10021 atcctcgcgg caatcggaat ctacggtgtg atgtcgtata cggtggtgca acgaacgcgt
    10081 gagctgggag tccgaacagc attgggcgcg acaccgcgct ccatcttgct actcgtcctt
    10141 ggcgaaggct ccaagtttgc gatgatcggg acggtaattg gaatcatcgg cgcagtggcg
    10201 cttcaccgga ccctgcagag cttgctcttc ggagttcgcg cgacgagcgt tctgacgctg
    10261 ggggccgcgg cggcgtttct ttttgcggtg gcgctgctag cgtgttacct gccggcacgg
    10321 cgggcgacgc gcgttgatcc acttgtagcg ttgcggtacg aataggagct attacatgca
    10381 caccctctgg caggacatcc gttacggcgc acgcatgctg tggaagcatc gccttgcgac
    10441 aattgtctgc gctacggcgt tggcgctggg catcggcgcg aatacagcga tgttcagcgt
    10501 ggccgaggca ttcctggtgc gttccgtgcc attcgaaaat cccgaccgca tcgtggccat
    10561 ggttaacgtg cggccgcagc agaacatcga caggaatgcg gtcgcgccgg caacttatct
    10621 cgattggcaa acgcaggcga agtccttcga acaattgggc gcgtacgaat atgctgaggt
    10681 gaaccttacg ggcaatcgcg agccggagaa agtccaggcg tttgaggtaa cggcaaattt
    10741 cttcggactc ctgggtgttc agcccaaaat gggccgaacc tttctgccgg aagaagaaca
    10801 gccgggaaaa gaccaggaga tcattctcag ctatggcctg tgggaacggc gctatgcgtc
    10861 cgatccgaat attctcaaca agatcgtcaa agtggatggc aagagtttca ccatcgtggg
    10921 agtgatggac aaagggtttg attttcccat aaccgcggaa gcgtggatgc cattggcgct
    10981 tacggtgaag cagcagtcgg agcggaattc gcgctacatc tgggtcttag gcaaactgaa
    11041 accggatacc tcgatcaagc aagcggtcgc agaaatgagc ggccttgcgc agcgcgaatc
    11101 cgatgcgtat ccggattctt acaagggttg gcagcttcgc gtgatgccgg tgcgcgaatt
    11161 tacaaccagt tatctaacgc gccagtacat tctccttctc atgggcgccg tcggcttcgt
    11221 gctattgatt gcctgcgccg acgtcgccaa cgtgcaattt gcgcgtgttt ccggccgtca
    11281 gaaggaactc gcggtgcgag ccgcgatggg ggcgagccga acgcgcatcg tgtgccaact
    11341 gcttacagaa agtgttcttt tggcgctgct gggctccgtg gcgggcttgc tcatcgcgca
    11401 atgggacctc gttctcatcc tggcgcacat gcccgcggac gtggccaagt atgttgccgg
    11461 atggaagacc atcagcctcg attccggcgc gtttcttttt actctggcca ttgcggtgtt
    11521 cagcggcatt ctgtctggaa tcgcgccggc gctgatgacg tcgcgcgcca acgtcagcga
    11581 aacactgaaa gaaagcggcc ggggatcttc ggcaggccgc gcgcgtcatc gcttgcgcaa
    11641 cgccctggtg gtggccgaga tttcgctttc gcttgtcctt ctagttggtg caggcctgct
    11701 ggtaaaaaat tttaccgccc tcgtcagtgt gaatgagagg tatcgcccgg aatcactcct
    11761 aacgctgaac ctgcacctgc cggaattgca atacaacacg cggtcgggac gtgtaacttt
    11821 ccacgagcaa gtgctgcaac ggctctcgac aatccccaac gttcaatcgg tggcgatggt
    11881 tacccacgtt ccgtattcgg atggcggagg agtgggcacg agaactttct ccatcgaggg
    11941 ccgcccggcg acagatcgcg gtgaagtgcg caatgcgatt gtccagacaa tcagcccaag
    12001 ctatctggat ctgatgcaaa ttgctttgcg tgagggacgc gagctgaccg aatcggatgc
    12061 cgatagcacg ctacccgtcg cggtgatcag caagagtctg gcccggcggt attttccgga
    12121 cgaaaaaccg ctgggaagaa aactgaaggt cgggcaagca gacgacagtt ctccgtggct
    12181 gacgattgtt gggatcgtgg atgacgtgca ttacagctgg atcgagaagg acgatgtccc
    12241 gacaatctac cgatcctacc ggcaatcgtc gcctttctac gcctcgctgg tgttacgcac
    12301 cggtggcgat ccactctctc tcgtctcttc ggtgcgaggc cagattgctg cggtggatcc
    12361 agacttgtct ctgtacaaca ttaagccgct gagcgtggtc atcagcgatt cgattgtcgg
    12421 tatagcttac gtcgccgtaa tgatgggcat tctggggatc atcgcgctag tgctcgcctc
    12481 ggtcggggtg tacggcgtga tgtcctatac ggtaagcgag cgaaccaacg aaatcgggat
    12541 ccgcatggcc atgggagcca cggcgagcga cattcaacgg ctggtgcttg gaaagggcgc
    12601 attgctcacg ctcattggca tgggcatcgg actgcccgtt gcgttcgttc tggcgacctt
    12661 tctctccagc ttgctcttcg gagtgaaggc tgccgatcca gtcgctttca tagtacttcc
    12721 gctggtgcta gcggccgtgg ccacattggc gtcctacctt ccagcgcggc gcgccgtgcg
    12781 cgtggatccg atcaccgcct tgcggtacga gtaggacgga atttgtgcgg caagaacata
    12841 cgctaagata gagacgcgat tcaccgttcg aaacgcaagg agccgatgtg gaacctttga
    12901 ttcaactgcg aaacatggaa aaagttgtcg aggccggtcc gaataagttt tatctgttgc
    12961 gcagaatcaa tctggatatt caacccggcg aatttgtcac catcatgggc ccctctggcg
    13021 ccggaaagtc tacgctgctg agcatcatcg gaatgctcga tggggcatgg agcggggact
    13081 attacttcca cggcgaaccc gtccacaagc tcaatatcaa gaagcggaac gaactgcata
    13141 aagcgaacat cggttttgtg ttccagagct atcatctgat cgacaatctg acggttttcg
    13201 aaaacctgga agtcccgctt tcctacaaga acgtgccgca caaggagcgg gttagcctgg
    13261 tttgcgacgc tctcgatcgc ttccacatcg ttggcaagaa agatctgttc ccgaaccagc
    13321 tctccggcgg tcagcaacaa cttgtcggcg tggcccgcgc cgtgattctc agtccgaaac
    13381 tgatactcgc cgatgagccg actgggaatc tccacaccgc acaaggcgaa gagatcatgc
    13441 agcttttcaa aagactgaat gacgccggta caacaatcat ccaggtgaca cactcagaga
    13501 gaaatgccgc ccatgggagc cgcattgtcc aactgaagga cgggtgggtt gtgggtgaca
    13561 acgccacagc cggcgcacaa gtcggcgcgg cacaacccgc gtgagcagac gatcgctggt
    13621 tgcaaaccgg cgctacgccc gcttctctcc gtgcctgaaa aatggcggta gggtaaactc
    13681 cctgaataca ttcgaatgaa gccgactaac ccgcaccctt atcgagttct tttggctgac
    13741 gaccaggcgg acatccgcga tgccctgcgc ttgctcctga agcgtgaggg ctatgaaacg
    13801 caggctctgg cttctccggc cgaggctctg agcgccatcg aatcgcggga atttgacgcg
    13861 gtcttgatgg acctcaacta cgcgcgagac acgacgtcgg gtcaggaggg gctcgacctg
    13921 ctcacgcgca tccaaatgct cgacagcacg ttgccggtta tcgtcatgac cgcatggagc
    13981 agcatcgaag tggctgtgga ggcgatgcgc cgcggcgcgc gcgactttgt ccagaagccc
    14041 tgggaaaata cccgcctgct cgccattctt cgcacgcaaa tcgatttgcg ccaagcgcta
    14101 aggcaggcct cacgtctcga agccgaaaac cgcatcttgc gcgccgaatc gcgcccgaca
    14161 tttcttgcga aatcccccgc catggtcccg gtgctggagc tgattggcca aatcggcccc
    14221 tcggacgcca acgttttgat caccggagag cacggcactg gaaaagaagt tgtcgctcga
    14281 acccttcatg cgttgtcggc acgcgcgacg aagccgatgg tgaccgtgaa tgcgggcggg
    14341 ctgtccgaag gtattttcga aagcgagctc ttcggtcacg taaaaggcgc ctttaccgat
    14401 gcgcgctcgg accgcgtcgg gcggttcgaa ctcgcggatg gcagcaccct gttcctagac
    14461 gaaatcgcca acgtgccgct caacttgcaa gcaaaacttt tgcgcgttct ggaaacgggg
    14521 gatcttgagc gtgtcggctc gtccaccact cggcgcgtca acgcgagagt gctggcggct
    14581 accaatgccg atgtgaatgc cgaggccgct gccggccggt tccgtcaaga cctcctcttt
    14641 cgcctgaata ccatcgagat ccacatccct ccgctgcgtg accgccgcga ggacatcgat
    14701 ctcctggcgc accattttct ccacggttat tcgcagcgct accgcaagag catttccggc
    14761 ttcgactcgg ccgccgtcaa ggctcttcac gagcattcct ggccgggcaa cgttcgcgaa
    14821 ctggatcacg cggtggaacg cggcgtgctc ctctcgcccg gcccttccat ccgcgcggca
    14881 gacctcggtt tgcgcgcgca ggccgccggc ggcgcggcca agctggagga gatgagtctc
    14941 gatgaagtgg agcggtatct catccagcgc actctctctc gccacgaagg aaatgtcagc
    15001 caggccgcca aggctctggg attgagccgc agcgccatgt atcgccggct gcaaaaacat
    15061 ggactggaat gacgcgactt acgtatgagc gccgcatcca gctcctcgcg ctagcggcgg
    15121 gcttcccagg ttcgctgatc gccatgattc tcttgtggca tggcaattat tcttctgggg
    15181 ctgtctggac acttgccttt ttcatctttt ctctctggct cggcttcgct ttctcccttc
    15241 gtcatcgtgt cgtcttttca ctgcaaacac tctcgaatct tctggccgct ttgcgcgaag
    15301 aagatttttc gctgcgcgct cgcggggcac gaagcgacga tgccatgggt gaagtgatga
    15361 ttgaagtcaa tgccctcagt gaaactcttc ggcaacagcg cttgggcgca ctggaagcca
    15421 ctgcccttct ccggaccgtc atggaggaaa ttgacctcgc catcttcacc ttcgacaaca
    15481 gcaccaagct gcggctggtc aatcgcgctg gcgaacgctt aatgggccgt ccggaaggtc
    15541 gcctgctggg atttaccgcg gaagagctgg gactcggcgc ttgtttggaa ggcgaagccg
    15601 cgcacaccat ggagctggcc tttcccggag gttccgggcg ctgggggctg cgtcgcggga
    15661 cgttccgcca gggaggcttg ccccatcatc tcgtagtcct gagcgatctg agccgcacgc
    15721 tccgtgacga agagcgtaaa gcgtggcagc ggctgattcg cgttctcggg catgagttga
    15781 acaactcgct cgcccccatt cagtccgtcg ctcaaggtct ggagagcggg ctactgactg
    15841 cctccaaatc ttccgaattg cagagcgaag cgtccgtttc gattctggat gatctccgac
    15901 agggtctcgg catcattcgc tctcgcaccg aggcgcttgg gcgcttcatg gccgcgtacg
    15961 cacaactggc gcggctgccg caaccaaagc tactgcctgt aaacgtctcg gaatggattg
    16021 gccggaccat aaaactcgag acccgagtaa aggtcagcct tttgaaaggc cctgatgtgg
    16081 tcgtttcggc cgacgccgac caactggaac agttgctgat caacctggta cgaaatgccg
    16141 cggatgcgac gctggaaacc ggcggctccg ttcaagttgg ctggtcccgg caaggcgcac
    16201 aactcgacgt ctgggtcatc gacgagggcc ccggtcttcc caatacgtcg aacctattcg
    16261 ttcccttctt tacaaccaag cagggtggct ccggtattgg tttggtgctt agccgccaga
    16321 tcgccgaagc tcatggtggt gagctcacct tgaaaaatcg ctccgacacc cggggctgcg
    16381 aagcccggct gcgccttccc atttagcggc ccgcccctaa cttctcttac ttggcccgct
    16441 caaccatgat ttggctttca caaaatcctt aggtacatct cattatttcc taagccttcg
    16501 cctgctactg tgtctctggc ctgatcgtga gcgttcggct cccttttcac cggagttctt
    16561 ctgtagtaaa cgagggcatt cgctcactgc ctctgcgaga tcgaggagat tcttgtgcaa
    16621 gatatgcttt tcgttgcggt aaccatcatc ttcttcgcca tcgcgatcct ttatattcgc
    16681 ggctgcgaac gcctgaaata ggacggagac ccgggatgct tggaaatatc ttgctcatcg
    16741 ttatctgcgt gtttttgttg gggtacctgc tggtcgctct gctgaaaccg gagaagttct
    16801 aacatgacgg caaatggctg gttccaaatc tttcttgtgc ttggagtgat ctttgcggtc
    16861 accaagccac tcggtgtctt catgacgcgg gtgttcaatc gggaaaagac gtttctggac
    16921 ccggttttgc gcccgatcga aaaactcgtc tatcgactga ccggcgtgga cgagaagcat
    16981 gagatgcgct ggacggaata cgccgtcgcc atgcttcttt ttagcggtgt ttccatggcg
    17041 ctcctgtatc tgatcgagcg gacgcagctg tggctcccgc tcaacccgca gaagctggcc
    17101 aatgtgccgc aagcgctggc gttcaacacc gcggcttctt ttacgaccaa taccaactgg
    17161 cagaactata gcggcgaggc ggccatgagt tacctaacgc aaatggccgg tctggcttac
    17221 cacaacttca cttcagcagc agcagggatc gtgatggcca tcgtggtcat tcgcggtatc
    17281 gcgcgcaagg aaaccgacaa gctcggtaat ttctgggtgg acatgacgcg ctgcctgctc
    17341 tgggtccttc tgccggtctg cctggtcggt tcgctgacgc tggtgtctca gggagttgtc
    17401 cagaacctga aaccgtatac aacggttgag cttactgaac cgcagaccgt gcaggtaact
    17461 ggcgcggacg gtaagacctc aacccaagtg gtgagccagc aggtgatcgc gcaaggtccc
    17521 gtcgcctcgc aggaaatcat caaggaattg ggcaccaacg gcggcggatt tttcaatgcc
    17581 aacagcgccc atccttttga gaatcccact cccctatcga actttctgga gcttgtgctg
    17641 atcttcgcga tctcctcggg cctcacttac acgctgggcc gcatgacagg ctctccccgg
    17701 cacggctggg ccgtgtgggc ggcaatggtg gtgctgtttt tgatgggcgt gacgacagcc
    17761 tattgggccg aggccagagg gaacccgata ttgactggcg tggatcagcg ggccggacca
    17821 ttgcagcccg gcggcaatat ggaaggcaag gaagttcgct tcgggatcgc caattccgcg
    17881 ctatttgcca cgatgacaac ggacaccagc tgcggcgcgg tgaacagcat gcacgactcg
    17941 tttactccgc tgggaggcat ggtgccctta atcaacatca tgctcggtga agttgtgttc
    18001 ggcggcgtcg gttccggact gtacggcgtg gtggtcttcg tgatccttgc cgtcttcatc
    18061 gccgggctga tggtcgggcg aactcccgag tacctaggga aaaaaatcga atcctacgac
    18121 gtaaaaatgg ccatgctttc gatcctaatt atgacgtttg tgatcctcgg cttctctgcg
    18181 atcgccattg tccagccatt tggcacctcc agcatctcca atccagggcc gcacgggctc
    18241 tcacaggtcc tctacgccta tacgtc
//
LOCUS       DASYVO010000079         3382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_101328, whole genome shotgun sequence.
ACCESSION   DASYVO010000079 DASYVO010000000
VERSION     DASYVO010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101328"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VGR03_02570"
     CDS             <1..1009
                     /locus_tag="VGR03_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV1993192.1"
                     /translation="KDVVGLFTSFTANDPQYLVRIDREKAKSLQVPFSQITNALQVYM
                     GSVYVNDFDFNNRSYRVYVQADSKFRSQPKDIGQYYLRSDTGKMISLDNLVTVEQTAN
                     PQVIPHYNLFRSAEINGTAAPGHSSGEGIAAMEALAKSTLPNGMAFEWSGLSREEIES
                     GGKALILFALGLVVVYLTLAAQYESYVLPFIVLLAVPVAILGAIGAQALRGLQNDVYC
                     QIGLVMLIGLASKNAILIVEFAEQLRGQGLSVVEASVEAARIRLRPILMTSLAFILGV
                     VPLVLARGAGRAGRVSVGTTVFGGMIAATTLNLVFIPVLYVIVRSIVPGRDAQAMPHG
                     S"
     gene            1154..2005
                     /locus_tag="VGR03_02575"
     CDS             1154..2005
                     /locus_tag="VGR03_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEV1993193.1"
                     /translation="MPLHTLSRRSFLAFSAMLPWALSARAAKSIPAGLELYSVRDELK
                     KDPEGTVRAVAQMGYQGVEFYAPYFEWSESQTKQMRKLLDDLGIRCFSTHNDSSYLNA
                     ENISRARDMNLILGSKYVVMASSNPRPGLDGWKAVADSLNAAADKLEASGLKAGYHNH
                     QPEFTVIEGQRPMEVLAKNTKPSIMLQLDVGTCLEAGSDPEAWIRANPGRIRSVHLKD
                     WSPEPAKGYTVLFGEGRADWKNIFEAAESAGGAEYYLLEQEGSRFSELETAQRCLQSF
                     LSMHGRA"
     gene            complement(2042..3349)
                     /locus_tag="VGR03_02580"
     CDS             complement(2042..3349)
                     /locus_tag="VGR03_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEV1993194.1"
                     /translation="MDSFWIGSVLVIALTGLYTVLGGMRAVAYNDAVQVFVLILGSAA
                     LTFYGLHQLGGWSELRHWCGSDMFNLWKPLIPPGVQGTWAPVLETNAAGKVIKEAWYF
                     NDYFPWLGMLFCAPIIGLWYWCTDQYIVQRALGAPNEKVARRGTIFAAFLKLTPVYLF
                     IIPGLICFALARSGKIPELAAMIGPDGKAIASAAQGAFPMMVQYLLPPGLRGLVVAGL
                     LSALMGSLAGVFNACSTLFTVDLYQKWKPEATQHQLVRTGRIATTVMVFIAMAWIPVI
                     KNAQGLYGYLQAVQGYLAPPIFVVFFLGVFFKRLNKQGALWAMIVGFVLGLFRMIVDT
                     PVTLGLSGLQNGYPHGSFLWIVNNIYFQYFSVLITLVSAIVMVGVSYMTAEPDYATLQ
                     NLTFATKTKEDKAATRASWDWREVAASALVLVCILGAYLYFRG"
BASE COUNT          748 a          956 c         1021 g          657 t
ORIGIN      
        1 caaggatgtc gtgggccttt tcaccagctt cacggcgaac gacccgcaat atctggtccg
       61 catcgaccgc gaaaaagcga agagtctgca ggtgcccttc agccagatca ccaacgcttt
      121 gcaggtctac atgggttcgg tctatgtgaa cgatttcgat ttcaacaacc gctcgtaccg
      181 cgtgtacgtg caggctgaca gtaaattccg ctcgcagccc aaagacatcg ggcaatatta
      241 tttgcgctcc gacaccggga agatgatttc gctcgacaac ctggtgacgg tcgagcaaac
      301 cgcaaatccc caggtcatcc cgcattacaa tctttttcgc tccgcggaaa tcaacggaac
      361 ggccgcgccg ggccacagtt ccggcgaggg catcgcggcg atggaagcgc tcgccaagag
      421 cactctgccg aacggcatgg ccttcgaatg gagcggattg tcgcgggaag aaatcgagtc
      481 cggaggaaaa gcgctgattc tttttgcgct gggcctggtg gtggtgtacc tgacgctggc
      541 ggcacaatac gaaagctacg tgcttccttt catcgtgctg ctggccgtgc cggttgcgat
      601 tctcggggcg attggcgctc aggcgctgcg gggattgcag aatgacgtgt actgccagat
      661 cggcctggtg atgctgatcg gcctggccag taagaatgcc attctgatcg tcgaatttgc
      721 ggagcaattg cgcggtcaag gcttgtcggt cgtcgaagcg tcggtggaag cggcgcgcat
      781 ccgtttgcgg cccatcttga tgacctcgct cgcgttcatc ctgggcgttg tgccattggt
      841 tctcgcgcgg ggcgcaggcc gcgcgggccg cgtgtccgtc ggcacgacgg tctttggcgg
      901 aatgattgca gcgaccacgc tgaacctggt cttcattcct gtcctgtacg taatcgtgcg
      961 gagcatcgtt cccggacgcg acgcccaagc catgccgcac gggtcatgag ctttttctac
     1021 ggccccgctt cgcacgcaag actacattgc caaactctgc cggctgaaaa aatatttccg
     1081 ttggtacgcc ttggcgaacg gcacagcgct aaaataaaat gaatttccgc tgaatctcca
     1141 gtaggattca gccatgccgc tacatactct ttcacgccgt tcctttctcg ctttttcggc
     1201 aatgctcccg tgggctcttt cggctcgcgc cgcgaaatcg attcccgccg gcctcgaact
     1261 ctattccgtc cgcgatgaat tgaaaaaaga tcccgagggc acggtgcgcg ccgtcgcgca
     1321 gatgggatat cagggtgtcg aattttacgc gccttatttc gagtggtccg agagtcaaac
     1381 caagcagatg agaaagcttc tggacgacct cggcatccgg tgcttctcga cgcacaacga
     1441 cagttcctat ttgaatgcgg agaacatcag ccgggcgcgc gacatgaacc tgattctggg
     1501 cagcaagtat gtggtcatgg cctcgtcgaa tccgagaccc gggctagacg gctggaaggc
     1561 ggtagccgat tctctaaatg ctgcggcaga taaactggag gcatcggggt tgaaggcggg
     1621 ttatcacaat caccagccgg agttcaccgt cattgagggc cagcgcccta tggaagtcct
     1681 ggcaaaaaat acaaaaccat cgatcatgct gcagctggat gtgggcactt gtctggaggc
     1741 cggttccgat ccggaggcgt ggattcgcgc gaatccgggc agaattcgct ccgtccatct
     1801 caaggactgg tcgccggaac cggcgaaggg gtacactgtt ttgttcggcg aaggccgagc
     1861 cgactggaaa aatattttcg aagcagccga aagcgccggc ggcgcggagt attacctgct
     1921 ggagcaggaa ggcagccggt tctccgaact ggaaaccgcg caacggtgtc tgcagagttt
     1981 tctctcgatg catggacgtg cataggagag tcatccgcag cgagactaaa ccagaattgt
     2041 ctcagcctct gaagtacaaa taagctccaa gaatgcacac gagaaccagc gccgatgccg
     2101 ccacttcgcg ccagtcccaa ctggcgcgcg tggctgcttt gtcttccttc gtcttggtgg
     2161 cgaacgtgag gttttgaagg gtggcgtagt ctggctccgc agtcatatag ctgacgccga
     2221 ccatgacgat cgccgaaacg agagtgatca ggacgctgaa gtactgaaaa tagatgttgt
     2281 tgacgatcca caggaacgag ccatgcggat agccgttctg aagaccgctc agaccgaggg
     2341 tcaccggagt gtccacgatc atgcggaaca gacccagaac gaaaccgacg atcatggccc
     2401 aaagcgcgcc ctgcttattc agccgcttga aaaacacgcc gaggaagaag accacaaaaa
     2461 tgggcggagc gagatagccc tggaccgcct gcaagtaacc gtagagcccc tgcgcattct
     2521 tgatgaccgg gatccacgcc atggcgatga agaccatcac agtggtggcg atgcgtccgg
     2581 tgcggaccaa ctgatgctgc gtcgcttctg gtttccactt ctgatagagg tccacggtga
     2641 agagcgtgga gcaggcgttg aacacgccgg ccagcgagcc catcaacgcg gaaagcagtc
     2701 cggccacgac caggccgcgg aggcccggcg gcaaaaggta ttgcaccatc atagggaagg
     2761 caccctgagc ggccgacgca atggccttgc cgtcggggcc gatcatggcg gccagctcgg
     2821 gaatcttgcc gcttcgagcg agggcgaagc agatcaggcc cgggatgatg aacaaataaa
     2881 cgggagtcag tttcagaaac gcggcgaaga tggttccgcg gcgggcgacc ttttcattgg
     2941 gagcgccgag ggcgcgttga acgatgtatt ggtcggtgca ccagtaccac agtccaatga
     3001 tcggggcgca aaacagcatg cccagccacg ggaaatagtc gttgaagtac caggcttctt
     3061 tgatgacctt gccggcggca ttcgtttcga gcaccggagc ccaagtaccc tgcacgcccg
     3121 gggggatcag gggcttccag agattgaaca tgtccgagcc gcaccaatgg cgcagctcgc
     3181 tccaaccgcc cagttggtgc aggccgtaga aggtcaacgc ggccgaacca aggatgagga
     3241 caaagacctg cacagcgtcg ttgtaagcca cggcccgcat gccgcccaaa accgtgtaga
     3301 gcccggtgag cgcgatgacg agtacggagc cgatccagaa gctgtcaatt tacatcccgc
     3361 cgatgtgcaa gcgcagctcg gg
//
LOCUS       DASYVO010000080         6389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_226947, whole genome shotgun sequence.
ACCESSION   DASYVO010000080 DASYVO010000000
VERSION     DASYVO010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226947"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            532..1053
                     /locus_tag="VGR03_02585"
     CDS             532..1053
                     /locus_tag="VGR03_02585"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEV1993195.1"
                     /translation="MRRFTAFLLILFCLASTSAAPHPDELAKKFVGAWRLVSIEGNPP
                     GRTNVYDRPTGLLMYNPSGRISVQIVVRADRKPFAPFSKGLLSATAEEKAAAFESYGA
                     YFGTYTVDTKAGTITHHLEDSLVPGRRGTDNIRWFEFHGEDRLSLVPVEDGKGGVIAR
                     KDAAYKLLWERIR"
     gene            complement(1473..3995)
                     /gene="priA"
                     /locus_tag="VGR03_02590"
     CDS             complement(1473..3995)
                     /gene="priA"
                     /locus_tag="VGR03_02590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV1993196.1"
                     /translation="MTPPFCNVALPVPLRTTFTYAVPPALQGTVQPGSRVLVPFRKKA
                     MVGVVVELAESAPHEAKIREIIRVLDFVPALTPKLIELAHWIAGYYLAPAGEVFRAML
                     PPVTELKSQRHIILTDAGRDAADSLSGGELSHGLTSGEAAFLAKLKEKKGTLPLGTAA
                     KLEVDALALQRLQRRALIEIRETVQGRKRRTQRVIAWKGRGVAVEESPEEKGEEKDNA
                     GKEKDNGETQSAQRKRREDRVRELLETERGPLPLPQLLKLAQVTRSFVERMLRDGMLE
                     SWEEPIDPAEDPFDVGYTPPAHELNAEQERALKGIRARFELGEFGVQLLHGVTGSGKT
                     EVYLRAVQDTLARGKTALVLVPEIALTLWIGRQCRSWFGARFEGVAVLHSALSDVERA
                     REWWRVRNGEARVVVGTRSAVFAPLENVGLIIVDEEQENSFKQEETPRYHGRDVAIVR
                     AKMQGALALLGSATPSMETYHHAREGKYELLTLASRVADRSLASVEIVDLREDFQQTH
                     QTSPISAALHAGIQECLANGTQALVLINRRGYSWSVLCRSCGASVQCVNCSISMTHHK
                     HRNRLECHYCGSIQQIPKQCPKCQSNYVYFFGEGSEHLEERLRKEFPGARIARLDRDT
                     ARTKRQYQETLGAFAGGALDILVGTQMLAKGHDFQRVTLVGVVSADSSLSLPDFRAAE
                     RTFQLLTQVAGRAGRGELKGRVLIQTFYPEHYAIQDAVKQDYSAFFERELHFRRMMAY
                     PPFTSLANVIVRDTSLEKAIRWSRQLSEYFSPHDDKDVRILGPATAPLARLKKEHRFQ
                     FLLKSPKRSVLTKLLSGAMAYCEAKEIPDTAVLVDMDPLSLL"
     gene            complement(4077..4949)
                     /locus_tag="VGR03_02595"
     CDS             complement(4077..4949)
                     /locus_tag="VGR03_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV1993197.1"
                     /translation="MGILLGLLTALTWGGADFIARFATHRIGALRSMLYMQLTGFLLL
                     SILLPTLGGWGHLADGSGWQPWAWGLLAGCLNAVSGLALYRAFEIGKMAVVAPLSASY
                     PALTLLLSWMTGERLTMVRIAGIICTLVGVVVVAGGEKTPDENDAAAVQRSGRGMGWA
                     IFAAVGFALLFWMLGIRIIPRVGAIQTVWMIRLTSTLLTAAAILVAKQPMRLPRGEVR
                     WMVLGMGVFDTGAFVLSNLGMKMEQVAVISVLGSLYGAVTVGLAAIFLKEHVSRWQWT
                     GIVTIFVGIFLISR"
     gene            complement(4956..5843)
                     /locus_tag="VGR03_02600"
     CDS             complement(4956..5843)
                     /locus_tag="VGR03_02600"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV1993198.1"
                     /translation="MAERISETLQKKLSGRLGFYEDLGIRLFYRDRGVVAAPDSERRL
                     APPATSHFSTLDLQKEETLPKPVGKPALQKTAPVVTPASPKIASLPVVAGPSLFEAVE
                     KIADDTLLKIREDLGECTRCKLHSTRHKIVFGDGNPKAELVFVGEGPGADEDAQGLPF
                     VGRAGKLLTQMIEAMGLQRKDVYICNVVKCRPPENRQPEEDEVSTCSPFLLRQIDTIA
                     PKVIVCLGAVAAKTLLQTNRGISQFRGEWLEFRGRKLLATYHPAYLLRNPPAKSEVWK
                     DLQKVMAVLGLEVKKGKSG"
     gene            complement(5851..>6389)
                     /locus_tag="VGR03_02605"
     CDS             complement(5851..>6389)
                     /locus_tag="VGR03_02605"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEV1993199.1"
                     /translation="VSGPVNLEVPAGVERIDVRTARDMHHAVVDRISGASIAILAAAV
                     ADYRPLEQHAEKIKKSDAALTISFEPTTDILAEVARTKGQKIVVGFAAETDHVAENAR
                     KKLSAKNADLIVANDVTAEGAGFDHDTNIVTLFSRDGRDLALPKMSKSEVAQRILDEV
                     VRLRSVLHGTAALKRSGV"
BASE COUNT         1321 a         2093 c         1595 g         1380 t
ORIGIN      
        1 ggaatccaac tcttactgcc cagttcttgg cgttctgcaa tttgcacagc atctcgacct
       61 gctgcgcact tttgaggcat ttcctcggga gacttggcgg ttgggggaga caatccatac
      121 ggcggcgacc aaccacttcc aacagtcatc ttcgggctgc ggttgtggtc tcgccagcgc
      181 cgctttgcac cattccccca gtgaccaacc gtcgcgtgcg cggacccatg ccccaaaata
      241 tgcaccgcgc atgccaaaga actcactgct tcatccgacc cttacctgcc aggctgtgcc
      301 atctatcttc gccaagccgc gacgctgcca tcttctttcc tttcaatgct gcgcaccgcc
      361 acttttcctc aagagtgttt tttctgtagc catctaagca ctagcagtcg cgccggattt
      421 cgcagtcgcc cctcttgaaa actgtctcaa ccagcgacac cctgtgctat cttggctcac
      481 tttgagattt gcaaacgctc cgtctctcga tctcaatcaa ggagctttcc gatgcgaaga
      541 ttcaccgcct tcctcttgat tctcttttgt ctcgcctcga catccgccgc gccgcacccg
      601 gacgagcttg cgaagaagtt tgtcggcgct tggagattgg tttccatcga aggcaatccc
      661 cccggtcgca caaacgttta tgaccggccc accggcctgc tcatgtacaa cccttctggc
      721 cgcatctccg ttcaaatcgt cgtcagggcg gatcgcaaac cctttgcgcc attttcgaaa
      781 ggattgctca gcgcaacagc cgaagaaaaa gccgccgcct ttgagagcta cggggcctat
      841 ttcggcacct acaccgtgga caccaaggcg ggaaccatca ctcaccacct ggaagacagc
      901 ctcgttcctg gccgccgggg cacagacaac atccgctggt tcgagttcca cggagaggac
      961 cgtctgtccc tcgttcccgt agaagacggc aaaggaggcg tgatcgcccg caaggatgcc
     1021 gcttacaaac ttctctggga gcgcatcagg tagcgctgag ggggagaaag aagtttccta
     1081 acggcttgaa atacaacagc ttgcctctaa cccttctttt ctcttgattt ctaattaaac
     1141 cgtgatatac ttatcttggg tatcttcatg ccacgattga gcttgcgagc tcgtcaataa
     1201 cctgatttct agtttctact ctctcttcga gtctaaccct tttcttttaa tgagcttgcg
     1261 cactctcttg catccaagta ttttttcggc cctttagaat caatcgctta cgcactcttt
     1321 tgcaagtttt tgcatttttt tgcatctctc aaaaatgcaa ctcttttctt ttcaagcgat
     1381 tccgcactct gcgtcaaaaa acacgctagt cgggctacct cccgatggac aatcctcatc
     1441 ctggactaga gcttctgcac ggaagccctt gattacaaca agcttagcgg atccatatcc
     1501 accagcaccg ccgtatcggg gatctccttg gcttcgcagt acgccatcgc gccgctcagc
     1561 aacttcgtca acaccgaacg cttcggcgac ttcagcagga actggaaccg gtgttccttc
     1621 ttcaaccgcg cgagcggcgc cgtcgccggt cccaggatgc gcacatcttt atcgtcgtga
     1681 ggtgaaaaat attccgaaag ctgccgcgac cagcggatgg ccttctccaa gctcgtatcc
     1741 cgcacgatca cgttggccag cgacgtgaac ggcgggtacg ccatcattcg ccggaagtgc
     1801 aattcgcgct cgaagaacgc gctgtaatcc tgctttaccg cgtcctgaat cgcataatgc
     1861 tcggggtaaa atgtctggat cagcacccgt cccttcagct cgccacgccc cgcgcgcccc
     1921 gcaacttgtg tcaacagttg aaacgtccgc tccgccgctc gaaaatccgg caggctcagc
     1981 gacgaatctg ctgaaaccac gccgacgagc gtcacccgct gaaaatcgtg ccccttcgcc
     2041 agcatttgcg ttccgaccag gatgtccagc gccccgcccg cgaacgcgcc cagcgtctcc
     2101 tgatactgcc gcttcgtccg cgccgtatct cgatcgagcc gcgcgattcg tgctccggga
     2161 aattccttgc gcagcctttc ttccaggtgc tccgagcctt ctccaaaaaa ataaacgtaa
     2221 tttgactggc acttcgggca ttgcttcggt atctgctgga tcgaaccgca atagtggcat
     2281 tccaaccggt tgcggtgttt gtgatgcgtc atcgagatgc tgcaattcac gcactgcact
     2341 gacgcgccgc aactccggca cagaaccgac catgaatacc cgcgccggtt aatcaacacc
     2401 agcgcctgcg tcccattcgc caggcattcc tgaattcctg catgcaacgc cgcggaaatc
     2461 ggactcgtct gatgtgtctg ctgaaaatcc tcgcgcagat ccacgatttc cacgctagcg
     2521 agcgaacggt cagcaacgcg cgaagccagc gtcagtagct cgtattttcc ttcccgcgcg
     2581 tgatgatacg tttccatcga gggagttgca gaacccagca gcgccagcgc gccctgcatc
     2641 ttcgcgcgca cgatagccac gtcgcggccg tgataccgcg gcgtctcttc ctgcttgaag
     2701 ctattttcct gctcttcatc gacgatgatg agcccgacat tttccagtgg cgcaaacacc
     2761 gccgagcgag tccccaccac gacgcgcgcc tcaccgttcc gcaccctcca ccattcgcgc
     2821 gcgcgttcca catcgctcag cgccgaatgc agcaccgcga cgccctcaaa ccgcgcgcca
     2881 aaccacgagc ggcactgccg cccaatccac aaggtcagcg caatttccgg caccaatacg
     2941 agtgccgttt tcccgcgcgc caacgtatcc tgcaccgcgc gcaaataaac ttcggtcttc
     3001 ccgctgccgg tcacgccgtg gagcagttgc acgccgaatt cgcccaattc aaaccgcgcg
     3061 cgaattcctt tcagcgcgcg ctcctgctcc gcattcaatt catgcgccgg aggcgtgtac
     3121 cccacatcga atggatcctc tgccggatcg atcggctcct cccaactctc cagcatcccg
     3181 tcgcgcagca tgcgctctac gaaagaccgc gtcacctgcg ccagcttcaa cagttgcggc
     3241 agaggcagtg ggccccgctc cgtttccaat aattcccgta cgcggtcttc tctgcgcttc
     3301 ctctgcgcgc tctgcgtctc tccgttatcc ttttcttttc ctgcgttgtc cttttcttcg
     3361 cctttctcct ccggcgattc ctcaacagcg acgccgcggc ccttccacgc gatcactcgc
     3421 tgcgtccttc gctttcttcc ctgcacggtt tcccgaattt caatgagtgc gcggcgttgc
     3481 agccgctgca acgccaaagc gtcaacctcc agctttgccg ctgtaccgag cggcagtgtt
     3541 cctttcttct ctttcaactt cgcgagaaac gccgcctctc cggacgtgag cccatgcgac
     3601 aattcaccgc cgctcaagct gtccgccgcg tcgcgccccg catccgtcaa aataatatgc
     3661 cgctgcgatt tcagctccgt cacgggcggc agcatcgcgc gaaagacttc gccggccggc
     3721 gccaggtaat accccgcgat ccaatgcgcc agctcgatca atttcggcgt cagcgccggg
     3781 acaaaatcca atacgcggat aatctctcga attttcgcct cgtgcggcgc gctctctgct
     3841 aattccacaa cgacaccgac catcgccttc ttccggaacg gcacgaggac gcggctcccc
     3901 ggttgcaccg ttccttgcaa tgcgggcggc accgcatatg taaacgtcgt gcgcagcggc
     3961 acgggcagtg cgacgttgca aaaaggagga gtcatcccgc cgtgtagtgt atcagcacga
     4021 ggaattgcgg aagaaagcgc ggaaaagaaa aaaagatacg ggatgaaaaa agaaatctac
     4081 cggctgatga gaaaaattcc gacgaaaatg gtgacgatcc ccgtccactg ccagcgcgac
     4141 acgtgttcct tgaggaagat ggccgcaagc ccgacggtga ccgctccgta aagcgagccc
     4201 agaacgctga tcaccgcaac ctgctccatt ttcattccaa gattgctgag cacaaacgca
     4261 ccggtatcaa acacgcccat gcccagcacc atccagcgca cttcaccgcg cggtaggcgc
     4321 atgggctgtt tagcaacgag gattgccgca gccgtaagca gcgtgctagt caagcggatc
     4381 atccacacag tttgaatcgc gccgactcgc gggatgatgc gaattccaag catccaaaac
     4441 aacaacgcga agccaacagc agcgaagatg gcccagccca ttccgcggcc gcttctctgc
     4501 acggctgcgg catcgttctc gtccggcgtt ttttctccgc cagcgacaac cacaacgccc
     4561 accagggtgc aaatgattcc ggcgattctc accatggtca gccgctcgcc ggtcatccag
     4621 gaaagcagca gtgtcaacgc cggataactg gccgaaagcg gagccacgac ggccattttc
     4681 ccaatctcaa acgcacggta gagcgccagt cccgagacgg cgttcaagca tcccgccagc
     4741 agaccccaag cccacggctg ccatccggaa ccatccgcaa gatgtcccca accaccgaga
     4801 gttggaagaa gtatggagag tagtaggaac cccgtcagtt gcatgtacaa catggaacgg
     4861 agagcgccaa tgcgatgcgt ggcaaaacgc gcaatgaaat ccgcgccacc ccaggtcagc
     4921 gccgtcagca ggcccagcag aattcccatg atgagttagc cggacttgcc tttctttact
     4981 tcgaggccga gcacggccat gactttttgg aggtctttcc agacttcgct ctttgcgggt
     5041 ggattgcgca gcaaataggc ggggtgatac gtcgccagca attttcttcc gcgaaactcc
     5101 agccactctc cgcgaaactg agaaattccg cgattggtct gtagcaatgt ttttgcggcg
     5161 acggcaccga ggcaaacaat cactttggga gcgatggtgt cgatctggcg caaaagaaat
     5221 ggcgagcagg tgcttacttc atcttcttcc ggctggcgat tctccggagg ccggcacttc
     5281 accacgttgc agatgtacac gtctttgcgc tgcaggccca tcgcttcgat catctgcgtc
     5341 agcagttttc ccgcgcgccc gacgaaaggc aatccttgcg cgtcttcatc ggcgcccgga
     5401 ccttcgccca caaaaaccag ctcggccttg ggatttccgt cgccgaaaac aattttgtgc
     5461 cgcgtgctgt gaagcttaca tcgcgtgcat tcgccgagat cctcgcgaat cttgagcagc
     5521 gtgtcgtctg caatcttctc cacggcttcg aaaagcgagg gtcccgcaac caccggcagg
     5581 gatgcgatct ttggactcgc tggcgtcacg accggcgcgg tcttttgcaa tgcgggtttt
     5641 ccgacgggct tcggcaaggt ctcttccttc tgaagatcta gagttgagaa atgggaggtc
     5701 gcgggcggtg ccaaccggcg ctcgctatcc ggcgcagcaa caacaccgcg gtctcgataa
     5761 aacaaccgga ttccaaggtc ttcgtagaat cccaacctgc cggaaagttt tttctggaga
     5821 gtttcggaaa tgcgctcggc catggcggga tcaaacgccg gagcgcttca gggcggccgt
     5881 tccgtgcaaa acgcttcgca aacgcacgac ttcatccaaa atccgctgcg ccacttcgct
     5941 cttgctcatc ttcggaagcg cgaggtcgcg tccatcgcgg gagaagagcg tcacaatatt
     6001 ggtatcgtga tcgaatcccg cgccttcggc ggtgacgtcg ttggccacga tcagatcggc
     6061 gttcttcgcg gaaagctttt tccgcgcgtt ctccgcgacg tggtccgtct ccgccgcaaa
     6121 gcccaccacg attttctggc ccttggttct cgccacctcc gccagaatgt cggtcgtcgg
     6181 ctcgaaagaa atcgtcagcg ccgcatcgct cttcttgatt ttttccgcat gctgttccag
     6241 ggggcggtaa tccgccaccg ctgcagcaag aatggcgatg gaagctccgg aaatccggtc
     6301 cacaacggca tggtgcatat ctctcgctgt gcgcacgtca atgcgctcta caccagcagg
     6361 cacttccaga ttcaccggtc cgctcacaa
//
LOCUS       DASYVO010000081         4111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_200314, whole genome shotgun sequence.
ACCESSION   DASYVO010000081 DASYVO010000000
VERSION     DASYVO010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200314"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VGR03_02610"
     CDS             <1..717
                     /locus_tag="VGR03_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEV1993200.1"
                     /translation="DYWRQLARHYASTNPELVTFEILNEPEFHDRYRWEGVQAKLAVA
                     IREGAPQHTIIAAGAYWSSESELLFFDPLHDSNIIYNFHFYDPHIFTHQGATWSTNYV
                     HYLKELPYPSTPENVQAAAALLPDAVNRLQAIHYGLDRWNAARIDGEIGQVAAWAKRW
                     NVPVTCNEFGVYRKAANPQDRAAWISDVRTTLEKYGIGWTMWDYSGGFGVVTKQEGHP
                     AVPDAVTVKALGLKMTPMPQ"
     gene            764..1249
                     /locus_tag="VGR03_02615"
     CDS             764..1249
                     /locus_tag="VGR03_02615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEV1993201.1"
                     /translation="MPVTLRAHEPRDFAALLRLDQGCFPPGIAYSKTMLRYFLKLPSA
                     DGMVAEDSGRIVGFILTEENPPLAHVITLDVAESHRQQGMGSALLAESEKNLALRGVR
                     TILLETATDNEAGVAFWQRHGYRIEATLRRYYLRKIDAYEMRKILPGTAKTAKHPGET
                     V"
     gene            1246..2130
                     /locus_tag="VGR03_02620"
     CDS             1246..2130
                     /locus_tag="VGR03_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV1993202.1"
                     /translation="MSDYLLSVLLGIVEGLTEFLPVSSTAHLRISEALFHVSLTSGYW
                     KMYTIVIQLGAILCLPVYFRERIRKLLDTFPQGDRGDRTFLTHPLSLVIVAFVVTAIP
                     AFLLTKVIGKHLESLYIMGSALLIGGIVMWIVDEMNGQSEAAGLGATGTRIHTWHMED
                     MIVLQAVWIGACQILSAVFPGTSRSMSTIAAGQVAGMSRASALEFSFFLSIPTMVVAT
                     CYDLLKSLMGKTENPIGVAQMNAHGWVVLAIGFVVSFIVAYGTVAWFMAYVRRRGFAP
                     FALYRIVVGSAVLFWASK"
     gene            2293..>4111
                     /locus_tag="VGR03_02625"
     CDS             2293..>4111
                     /locus_tag="VGR03_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEV1993203.1"
                     /translation="MRFLTPTGNKRLNELIGFLCITLAVLIALALLSYSPKDAAFNVS
                     APAPDGSPARNWIGPAGAYGADLLFQIFGFAAFLLPATILVLGWRWCRSRTIDSQVAT
                     LIGYGLLLLSFPALISLLSFIPAVRGSIPAGGFVGALVSSGLLASVKWGAYLVAPAML
                     VAAIFMTTRFSFSGAHAWASGPNGPIGKVERLGILQKAQARWHAWREEREQQRMRRRV
                     EESRLSGRKPVPPQSIGKAEVLNEPPKTIRLSDQSNIFNGKAEIEEEEETKDASHKSP
                     TFFLNRETDKAAAKKAGEPKIAKGSPNYRFPSPSLLREGERSQKLDEDELKLRARAIE
                     AKCLEFDIQGRVTQINPGPVVTTFEFKPDAGIKYSRITGLTEDLCLALQAESILIERI
                     PGKSTIGIEVPNDKRQTIALRDIIEAPEFSNSPSKLTLAMGRDLHGRIRVTDLAAMPH
                     LLIAGSTGTGKSVFINSLLMSILYKASPEEVKLVLVDPKRLELNLYENIPHLIAPVVT
                     DPKIASNVLRNATREMENRLKLLAQRGVRNIDQYNRTFKKEQSLSLFDNLEETEHKPL
                     PYLIIVIDELADLMMVDTNNVEESITRLAQMARAVGIHLI"
BASE COUNT          865 a         1098 c         1264 g          884 t
ORIGIN      
        1 gactactggc gtcagctagc gcggcattac gcttccacga atcccgagct tgttacgttc
       61 gagattttga acgagccgga atttcacgac cgctatcgct gggagggcgt gcaggcaaaa
      121 ctggcggtgg cgattcgcga aggggcgccg cagcatacga ttatcgcagc aggagcgtac
      181 tggtcttcgg agagtgagct gctgtttttc gatccgctgc acgattcgaa catcatctac
      241 aactttcatt tctacgaccc gcacatcttc acgcaccagg gcgcgacctg gagcacgaat
      301 tacgtgcact atctgaaaga gctgccgtat ccttcgactc ccgagaatgt gcaggcggcc
      361 gccgcgctcc ttccggatgc ggtgaatcgc ctacaggcga ttcactatgg actggaccgc
      421 tggaacgctg cgcgcatcga cggcgaaatc ggccaggtgg ccgcgtgggc gaaacgctgg
      481 aacgtgccgg tgacttgcaa cgagtttggc gtctatcgca aagccgcgaa tccgcaggat
      541 cgcgcggcgt ggatttccga tgtgcgaaca acgctggaga aatacggaat cgggtggacc
      601 atgtgggatt acagcggcgg gtttggagtg gtgacgaagc aggagggcca tccagccgtt
      661 cccgacgccg ttacggtgaa ggcgctgggg ctgaagatga ctcccatgcc acagtgattg
      721 agccaggctg ttcagaggga agggttcggc ctacaatcgc gccatgccgg tcacgctgcg
      781 cgcccatgaa ccgcgggact ttgctgccct gctgcggctc gaccaagggt gctttcctcc
      841 ggggatcgcc tactcaaaaa ccatgctgcg gtacttcctg aagctgccgt ccgcggacgg
      901 aatggtggcg gaggactcgg ggcggatcgt ggggtttatt ttgacggagg aaaatccgcc
      961 actggcgcac gtgattacgc tggacgtggc ggaatcgcac cgccagcagg ggatgggctc
     1021 cgcgctgctg gcggagagcg agaagaacct ggcactgcgc ggagtgcgaa cgattttgct
     1081 ggaaacagca accgacaatg aagccggcgt tgccttctgg caacggcatg gctatcgtat
     1141 cgaagcgaca ttgaggcggt attacttgcg gaaaatcgac gcgtatgaaa tgcggaaaat
     1201 tctgccgggc acggcaaaaa cggcaaagca tccgggagaa acagtttgag cgactacctg
     1261 ttgtctgtgc tgctgggaat cgtcgaagga ttgacggaat ttttgccggt cagctcgacg
     1321 gcgcatctgc ggatttccga agccctcttc cacgtaagcc tcacgagcgg ttactggaag
     1381 atgtacacga ttgtgattca attgggcgcg attctttgct tgcctgttta ttttcgtgaa
     1441 cgcatacgga agctcttaga tacttttccg caaggcgacc gtggcgaccg cacgttcttg
     1501 acgcatcctc tgagcctcgt catcgtggcc tttgtggtga ccgcaatccc tgccttcttg
     1561 ctcaccaaag tgattggcaa gcatctggag agtttgtaca tcatgggcag cgccctgctg
     1621 atcggcggaa tcgtcatgtg gattgtggac gagatgaatg gccagtcgga agccgcggga
     1681 ctgggagcta ccggaacgcg gattcacacg tggcatatgg aggacatgat tgtgttgcaa
     1741 gcggtgtgga tcggggcctg ccagattctt tcagcggtgt tcccggggac ctcgcggtcg
     1801 atgtccacaa tcgcggcggg gcaggtggcg ggaatgtcgc gggcttcggc gctggaattc
     1861 tcgttcttcc tttcgatacc gacgatggtg gtggcgactt gctacgacct gctgaagtcg
     1921 ctcatgggga agacggagaa tcccatcggc gtagcgcaga tgaatgcaca tggatgggtt
     1981 gtgctggcca tcggcttcgt ggtttcgttt atcgtcgcgt acggtacggt ggcgtggttt
     2041 atggcctatg tgaggcggcg tggatttgct ccttttgcgc tgtacaggat tgtcgtgggg
     2101 agcgcggtat tgttctgggc atcgaaataa actggtttcg ctttcttaga aacaacaagg
     2161 aaaaacaaat tttcgtttgt aacgggttct ttggtatatt cagggctgtt cgggagttca
     2221 tccaggcggg attacgctag cgccagcccc cgccacgaca ctcagccttc ccctaaaagc
     2281 tgaggagacc ttgtgcgctt tctgactcca actggaaata agcggctcaa tgagctgatt
     2341 ggatttttgt gtatcacgct ggcggtgctg atcgcactgg ctttgctttc gtattctccg
     2401 aaggacgcgg cgtttaacgt aagcgctcca gcgcccgacg gcagtccggc gagaaattgg
     2461 atcggaccgg ccggggcgta tggcgcggat ctcctttttc aaattttcgg atttgcagcg
     2521 tttttgctgc cggcgacgat tctcgttctg ggttggcggt ggtgccgcag ccggacgatc
     2581 gattcgcagg tggcgacgct catcggctat gggttgcttc tactttcctt tcctgcgctc
     2641 atttccctcc tatccttcat tcccgccgtc cggggatcca taccggcggg cggctttgtg
     2701 ggagcactgg tgtccagcgg cctgctggcc agcgtgaaat ggggcgccta ccttgtggct
     2761 ccggccatgc ttgtagccgc gattttcatg acgaccagat tttctttctc cggcgcgcat
     2821 gcctgggcca gcggaccgaa tggaccgatt ggaaaagtgg agaggctggg cattttgcag
     2881 aaggctcaag cacgctggca tgcctggcgc gaagaacgcg aacagcaacg catgcgccgt
     2941 cgcgtggaag agtctcgcct atcgggaaga aaacccgttc cgccacaatc gattggaaag
     3001 gccgaagtcc tgaacgagcc tcccaagacg attcgccttt ccgaccagag caatattttc
     3061 aatggtaagg ccgaaatcga agaagaggaa gagacaaagg acgcatccca caagtcgccg
     3121 accttcttcc tgaatcgcga aacagataag gccgcggcta aaaaagccgg cgagcccaaa
     3181 atcgcgaagg gcagtccaaa ttatagattc ccttcccctt ccctgctgcg cgagggtgag
     3241 cgcagccaga agctggatga ggacgagctg aaactgcggg cgcgcgcgat tgaagcaaaa
     3301 tgcctcgaat tcgacatcca agggcgcgtg acgcaaatca atcccggacc ggtggttacg
     3361 acattcgaat tcaaaccaga tgcgggcatc aagtacagcc ggattaccgg ccttaccgaa
     3421 gacctgtgtt tggcgctgca ggcggaatcg atcctgatcg agcgcatccc gggtaaatca
     3481 acgattggaa tcgaagttcc gaacgacaag cggcagacga tcgcgctgcg ggacattatc
     3541 gaggcgccgg agttcagcaa ttccccgagc aagctgacgc tggcaatggg tcgagatttg
     3601 cacgggcgga ttcgcgtgac ggacctcgcg gctatgccgc acttgctgat tgccggttcc
     3661 accggcaccg gaaagagcgt ctttatcaac tcgctgctga tgtcgattct ctataaagcg
     3721 agtcccgagg aagtgaagct ggtgctggtt gaccccaagc ggctggaact caatctttac
     3781 gagaacattc cccacttaat cgcgccggtg gtgaccgatc cgaagatcgc atccaacgtg
     3841 ctgcgaaatg ccacgcgcga aatggagaac cgcttgaagc tgctggcgca gcgcggcgtt
     3901 cgcaacatcg accagtacaa tcgcacgttc aagaaggagc agtcgctctc gctcttcgac
     3961 aatctggaag agacggagca caagccgctt ccctacttga tcatcgtgat cgacgagttg
     4021 gccgacctga tgatggtgga caccaacaat gtggaagagt ccatcacgcg gctcgcgcaa
     4081 atggcgcgcg cggtgggcat tcacttgatc c
//
LOCUS       DASYVO010000082         5487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_16609, whole genome shotgun sequence.
ACCESSION   DASYVO010000082 DASYVO010000000
VERSION     DASYVO010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16609"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            444..956
                     /locus_tag="VGR03_02630"
     CDS             444..956
                     /locus_tag="VGR03_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993204.1"
                     /translation="MKMPKKASTTKNDQQSLAPTPPPSPNRCQFETSDGRRCRMLRHN
                     AHPALCLFHARDEMQLLESQRLGAELSASITGQFMTATDINFVLGKLFTATAQNRIPP
                     RNAAILAYVAQLMLHSLSGVKKEYPFTYKYEVWNNMLEEATPLSNSTPDDLAPAPDSP
                     DDPDNDPEKS"
     gene            complement(1325..2473)
                     /locus_tag="VGR03_02635"
     CDS             complement(1325..2473)
                     /locus_tag="VGR03_02635"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEV1993205.1"
                     /translation="MKRMMMLMIFTGALLMTTMAPIGATPLPGSTTKKSFGKTPDGQP
                     VDLFVLTNKGGAEASITNYGGAVVSLKVPDRNGKLADVVLGYDNADGYVADKSFFGAL
                     IGRYGNRIGHAQFVLDGKTYTLAKNNGENSLHGGIKGFNKALWSAKTISTRGGQSVEL
                     SYLSKDGEEGFPGNLKVTVIYTLTNTNALKIEYSATTDKKTVVNLTNHSYFNLAGQGS
                     GDILGHLLMIPADQFTPVDAGLIPTGELRDVAGTPFDFRKPMAIGARIDRDEEQLKLG
                     GGYDHNFVLRPPMDRAEFLAARVVEPTTGRVMEVWTTEPGVQFYTGNFLDGKTAGKGG
                     VAYPHRSALCLETQHFPDSPNQPKFPSAVLKPGARYHTITTYKFSTEK"
     gene            complement(2566..3480)
                     /locus_tag="VGR03_02640"
     CDS             complement(2566..3480)
                     /locus_tag="VGR03_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV1993206.1"
                     /translation="MTKLRFLVSLITKDNDYQMEQAAAAKTAAAELGVEAEIVYADND
                     PITQSTQVLKVIQTEPSLRPSGIIIEPLGATSFPQVARAAASAGIGWAVLSREAEYAG
                     ELRKTIRTPVFSVSADQIEVGRIQARQVAALLPRGGAVLLIQGPSVSSVSRDRHTGLL
                     ELLAPNVHVTSLRGRWTEESAHQSVCSWLRLMAAQKSRIDLIVAQNDAMGMGARKAID
                     ETILDRDRDRWLGIPITGCDGVPRTGQTWVRTGQLAATVVVPPSTGVAMTLMTKALLS
                     GTAVPEHFFTTPSPFPAIDKLVPRGSGG"
     gene            complement(3562..5145)
                     /locus_tag="VGR03_02645"
     CDS             complement(3562..5145)
                     /locus_tag="VGR03_02645"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HEV1993207.1"
                     /translation="MAVVAGVDFGTLSVRVSIVDSERGLLASAIAEYPLHRKREDPEY
                     ATQSHEDHMRALASATREVVRKSGVAADRILALALDTTGSSVIPVGKNLQPLDDYYLW
                     CDHRAKAEAAEITAVAHREKLAAIDWCGGVYSSEWGFSKLLHWLRHNPAKRSEFVSAF
                     EHCDMVAATLCGITDAKQVKRSVCAMGHKWLWNASLGGLPPEDFLIKVDLLLAGVREK
                     IDGEYAASDRLAGRLSPHWAQQLGLKPGIPIPVGAFDAHWDAIGAGCRSEDIVNVVGT
                     STCIIGITPSVRLVPGVCGVVQGSVHPQRTGIEAGLSAVGDIFQSIATRAGTDVPTLC
                     RGLENYRAGQTGLLRITWDNGDRTVLVNPNLRGITFGWNLQTTAQDELFAAIEGTALH
                     TRVILDRMEEHGVIVRRVINAGGIPQKNDVLNQVYANVLGRPVLVPAGNVVSLGSAIF
                     AFLAAGVFKTVEEAQDKICPQYKVFQPEIFAKGVYEKLYAFYSKIYFAFGRPQNAEFG
                     NLLPGLIQLSESAHADAGR"
BASE COUNT         1151 a         1593 c         1585 g         1158 t
ORIGIN      
        1 aagagcaaag attctttcag gtaagaagaa aacacgagcc ccgcggctcg cacactctaa
       61 gtgtctcttg acagagccga cgccatcata gagggtggcc ctcagatcgc ccgagttcga
      121 gtttcgggtg gcccattttt cgcgcttcgc gaaaggtggg gcctttgact tttccacaga
      181 tttccacaga tctttcgaat cgtgccctcc cgcaaaccct ttccctcggc caacttgacc
      241 cccactgtgt caaccttgcc acatccattc gaatcactac atagttatcc ttgacaggcg
      301 ttatccatat cgctatactg tttcttgata gttcgagccc attctcggct ccattccggc
      361 ggcccgccgc gcctcgtaat ctctttcttc ttttcgttac gtcattacct ctctacttcg
      421 ttacttcaaa tcaaggatct ctcatgaaaa tgcccaagaa agcatccacc accaaaaatg
      481 accagcaatc tctcgcacca actccaccgc ccagccccaa tcgctgccag ttcgagactt
      541 ccgatgggcg ccgctgccgc atgctccgcc acaacgctca ccccgcgctc tgtctcttcc
      601 acgctcgcga cgagatgcaa ctcctcgaat cccagcgcct tggcgccgag ctctccgcct
      661 ccatcaccgg ccagttcatg accgctaccg acatcaactt cgtcctcggc aaactcttta
      721 ccgccacggc ccagaaccgc atcccgccgc gcaacgccgc cattctcgcc tacgtcgccc
      781 agctcatgct ccactccctc tcgggggtca aaaaggaata tcccttcacc tataaatacg
      841 aggtgtggaa caacatgctc gaggaagcca ccccgctctc caattccacc cccgacgatc
      901 ttgctcccgc tcccgactcg cccgacgacc ccgacaacga ccccgaaaag tcctgacccc
      961 tcccatgtgc ccccttctta gttcctccac ttttataggg gcgcaataca ttgcgccctc
     1021 tctttctgtc tccgtgatct ctgtgttttc tgtgcgctct gtgttaagtc ctttagaatg
     1081 cgcagttccg caaatcgcgc ccataactcc tttagaatgc gcagttccaa aaacacggca
     1141 cttaaagtcc tttagaatgc gcagttccga aaaaagtggg ggggaggggg gtaaattgtt
     1201 aaccagacac ccgatgaggg gtgtctgtcc tgagcgacca tcgggagcga aggatctctc
     1261 ttcaagaccc acgaggatca ctgtcccgtg ggtccatcgg gagccgacag ccaggcgcca
     1321 gccgtcactt ttcggtggaa aacttatacg tggtgatcgt gtggtagcgc gcgccgggct
     1381 tcaggaccgc ggacgggaat ttcggctgat tcggggaatc gggaaagtgc tgcgtctcca
     1441 ggcagagcgc cgagcggtgc ggataggcca cgccgccctt gccggcagtc ttgccgtcga
     1501 ggaaattgcc ggtgtaaaac tgcacgccgg gctcggtggt ccagacttcc atgacgcggc
     1561 cggtagtggg ctcgacgacg cgcgcggcga ggaattccgc gcggtccatc ggggggcgca
     1621 ggacaaagtt gtggtcgtag ccgccgccga gtttcaactg ctcttcgtcc cgatcgatgc
     1681 gcgcgccgat ggccatcggt ttgcgaaagt cgaacggcgt gccggcgacg tcgcggagtt
     1741 ccccggtggg gatcaggccg gcatcgacgg gagtgaactg gtcagccgga atcatgagca
     1801 ggtggccgag aatgtcgccg gaaccctgac cggcgagatt gaaataagag tggttagtga
     1861 gattcaccac ggttttcttg tcggtggtcg cggaatattc gattttcaat gcgtttgtat
     1921 tcgtgagggt gtagatcacg gtgactttca aatttccagg gaaaccttcc tcgccatcct
     1981 tgctgaggta ggaaagttcg accgactggc cgcccctcgt ggaaattgtc ttggcgctcc
     2041 acagcgcttt attaaaaccc ttgatgccgc cgtgcagact gttctcaccg ttgttcttgg
     2101 cgagcgtgta ggtcttgccg tcgagcacga attgcgcgtg cccgatgcgg tttccgtagc
     2161 ggccgatgag cgcgccgaaa aaacttttgt cggcaacgta gccgtcggcg ttgtcgtagc
     2221 cgaggaccac atccgcgagc ttgccgttgc ggtccggcac cttcagagag acgaccgctc
     2281 cgccgtagtt agtgatggag gcttccgcgc cgcctttatt ggtcagcaca aaaagatcga
     2341 cgggctggcc gtcgggagtt tttccgaagg acttcttcgt ggtggaaccg ggcagaggtg
     2401 ttgcgccaat cggcgccatg gtggtcatta gcaaagctcc cgtgaagatc attaacatca
     2461 tcattcgttt cattggaatc ctccacccgt cagtattttc gattacaaca ccccgaagtt
     2521 ccagtaagtt tacttgctga tgcactcttt ggctcgcaaa attattcatc cacccgatcc
     2581 ccgtggaacg agcttgtcga tcgcagggaa tggcgaggga gtggtaaaga aatgctcggg
     2641 aacggcggtc ccagagagca gcgctttggt cattaaggtc atagctactc ccgtgctggg
     2701 cggcacaacc acggtggcag ccaattgccc ggtgcggacc cacgtctggc cggttctggg
     2761 cacaccatca caaccggtga tgggaatgcc gagccagcga tcacggtccc tatcgaggat
     2821 ggtctcgtcg atggctttgc gcgcgcccat gcccatggca tcgttctgcg cgacgatgag
     2881 atcgatgcgc gacttctggg cggccatcaa acggagccac gaacaaacgc tctgatgagc
     2941 gctttcctcg gtccagcggc cgcgcaggct ggtgacatgc acgttgggcg cgagcaattc
     3001 cagtaaaccg gtgtgacgat cgcgggagac cgaacttacc gaaggcccct gaatgaggag
     3061 cacggcgccg ccccggggaa gaagtgcggc aacctgccgg gcctgaatgc ggccaacttc
     3121 gatctggtcg gcgctgacgg aaaaaacggg agtgcgaatg gtcttgcgaa gttcgccagc
     3181 gtactccgct tcgcggctga ggacagccca accgataccg gcgctggcgg cggccctggc
     3241 gacttgcggg aaagaagtgg caccgagagg ttcaataata atgccgctgg ggcgaagcga
     3301 gggctccgtc tgaatgactt tgagtacctg agtgctctgg gtgatgggat cgttgtcggc
     3361 ataaacgatt tcagcttcga cgccgagctc cgcggcggcg gttttcgcgg ctgcggcctg
     3421 ttccatctga tagtcgttgt ccttggtaat cagcgagacc aggaaacgta gcttggtcat
     3481 ccatttcctc cgcagtccga taaacgccga accaccagcg cgaagaagga aaaaagaatt
     3541 gcgattccag ccatcgatgg attagcgccc ggcatcagca tgcgccgatt ccgacaattg
     3601 aatcaagccg gggagcagat ttccaaactc ggcgttttgc ggccggccga aggcaaaata
     3661 gattttgctg tagaaggcat agagtttttc gtaaacgccc ttcgcgaaaa tttcgggttg
     3721 aaacacttta tattgagggc agattttgtc ttgcgcttcc tcaacggtct tgaagacgcc
     3781 ggcggcgaga aaagcaaaaa tggccgaacc gaggctgacg acgttgcccg cgggaacgag
     3841 cacgggcctg ccgagcacat tggcatagac ctgattcaga acatcgtttt tctgcggaat
     3901 gccgccggcg ttgatgacac ggcgaacaat gacgccgtgc tcttccatgc gatcgaggat
     3961 gacgcgggta tgcaaggcgg tgccttcgat ggcggcgaac aattcgtcct gcgcggtggt
     4021 ttgcagattc cagccgaagg taatgccgcg caggtttgga tttacgagca cggtgcggtc
     4081 gccattgtcc caggtgatgc ggagcaggcc ggtttgtccg gcgcgatagt tctccagtcc
     4141 acggcagaga gttggcacat cggttccggc gcgggtggcg atggattgaa aaatatcgcc
     4201 gacggcggag aggccggctt caatgccggt tcgctgcgga tgtacgctgc cttgaactac
     4261 gccgcataca ccgggaacca gacgcacgga gggagtgatc ccgatgatgc aggtcgaggt
     4321 tcctacgacg ttaacgatgt cttcgctgcg gcagcctgcg ccaatggcat cccaatgggc
     4381 gtcgaacgcg ccgacaggaa tggggatacc gggctttaat ccaagttgtt gcgcccaatg
     4441 gggcgagaga cggccagcga gccgatcgga agcggcatat tcaccgtcga ttttttcgcg
     4501 cacgcctgcc agtagtagat ccaccttgat gaggaaatcc tctggtggca gaccgccgag
     4561 cgaagcgttc cacaaccact tgtggcccat ggcgcagacg ctgcgcttca cttgctttgc
     4621 atcggtgatg ccgcagagag ttgcggcgac catatcgcaa tgttcgaaag cagagacgaa
     4681 ttcagagcgc ttcgcgggat tatggcgaag ccaatggagg agtttcgaaa agccccactc
     4741 ggaagaatac acgccgccgc accaatcgat cgcggcaagc ttctcgcggt gagcgaccgc
     4801 ggtgatttcc gcagcttcgg ctttggcgcg atgatcgcac cacaaataat agtcatcgag
     4861 cggctggaga tttttgccaa cgggaatgac ggaggagcca gtggtatcaa gagcgagggc
     4921 gagaatccga tcggccgcaa cgccggattt ccggacgacc tcgcgagtag cggacgccag
     4981 ggcgcgcatg tgatcttcat gcgactgcgt ggcgtactcg ggatcttcgc gtttgcggtg
     5041 gaggggatat tccgcgatgg cggaagcgag cagccctcgc tcgctgtcca cgatggaaac
     5101 gcgaacgctc aatgttccaa aatccacgcc ggcaacgacc gccatgtacg ctctccgctg
     5161 atgtcctttg cgctagtctg ttttccgact atcgatcaaa tgagtcttct gtccgggcaa
     5221 aaactcgacg cggcaaacca aaccgagaac tggcaatatc aaccgccgac agatggacga
     5281 gcgccgccgg tacaatcgca gccatcgctg cgatcatttt ttctctctag gctgcccgta
     5341 gtaggcagcc tttccatgtt tcctgagata gtgctggtcg tgaagaaaac gtccgatagc
     5401 ggtgacttgc ggattgatgc cgagagtgaa gtacgccatg cgcgcgacgg attccagaat
     5461 gacggcgttg tgagcggacg ttgcggg
//
LOCUS       DASYVO010000083         3578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_256598, whole genome shotgun sequence.
ACCESSION   DASYVO010000083 DASYVO010000000
VERSION     DASYVO010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256598"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VGR03_02650"
     CDS             complement(<1..346)
                     /locus_tag="VGR03_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1993208.1"
                     /translation="MNAERGTQTVLITGATDGLGKAAALLLAKRGYRVFAAGRSAEKR
                     AQLDALAKEKKVPLETLEMDVCDDISVQRAVASVYQKSGTIDVLINNAGLVYVGAVED
                     LRMEDWRRQFETN"
     gene            519..>3578
                     /locus_tag="VGR03_02655"
     CDS             519..>3578
                     /locus_tag="VGR03_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1993209.1"
                     /translation="MPADEDLCIPAPEKKWRLGFWSLIATQFQGAFNENGLKFLVIYL
                     ILAVEKDKAQRDQLELLVGVLFAAPFILFSLTGGYLADRFSKRAVTIWTKVFEVGVML
                     FAAAALVGPNLKLALAAIFLVCTQGALFGPSKYGLLPELLPQERLSWGNGVLELGTFV
                     AIIIGSVSGSFLAEAFDGREVYSGALLLGFTIVGLAWSFGISRVPAADPAKRFHLTSL
                     IDVWSPMKVIRGDRVLWLAVLGNTYFFFVGGLLQFNIFIYGQDVLHIRSTQGGLLQAA
                     VAIGIGLGSFAAGYLSGGKIEYGLIPLGSLGMTALGLCLAIPDLSFRAVLLLLAALGF
                     FGGFFIVPISALIQHQPEEDKKGVVIGTANWLSFVGIGAASGMYYAATHFAHLSPGGI
                     FFWSAFATLGATAYVLWLLPDSLLRLILWFATHTLYRLDVEGRENIPARGGALLTPNH
                     VSMADAVLLIASIDRPIRFLMFKGSYEHPLVKPFAKIMGVIPIAPDQGPREMIHSLRL
                     ATDALKNGEVVCIFPEGQMTRIGQMLPFRRGMERIIKGVDVPIIPVNLDGVWGSIFSF
                     AGGRFLWKFPRQIPYPVRVTFGKPLPSTATAQDVRRAVQDLGAEAFARRKKRMHTLPQ
                     SFIYSARRHPFRFAMADGQRPKLSWFSALVGALVLARRLRKQWQGQEMVGILLPPSVP
                     GALVNYAAMLLGKVPVNLNYTVSNETLASCAQQCNLKTIVTARVFLEKVKIQPPGETI
                     FIEDVVKAAGFGERVAAALVASFLPAKAVAKFAGCDRPASLDDIATIIFSSGSTGDPK
                     GVILTHYNIASNVEQLNQVFMLHAEDRIMGILPFFHSFGFTGTLCLPAATGIGVVFHP
                     NPLDSRVIGALVNKYAVTMLLATPTFLNAYTRRCTPEDFGSLRFVMAGAEKLPDRISQ
                     AFEDQFGIRPHEGYGCTECSPAVTVNTIDFRAASFLQVGAKRGSIGHPLPGITVKIVD
                     PDTLQPVGVDEPGLLLVRGPNIMLGYLNKPEKTAEVLRDGWYNTGDVA"
BASE COUNT          654 a         1057 c         1047 g          820 t
ORIGIN      
        1 agttcgtttc gaattgccgc cgccagtctt ccatgcgcag atcctcgacg gcgccaacat
       61 agaccagccc cgcattgttg atcagcacgt cgatcgtgcc tgacttctga tagacggaag
      121 caacggccct ttgcaccgag atgtcgtcgc acacgtccat ctccagggtt tcgagcggaa
      181 ctttcttttc cttggccaac gcgtcgagct gcgcgcgctt ctctgccgag cgcccggccg
      241 caaagacgcg gtagcctcgc ttggcgagca acagcgccgc agccttcccc aatccatccg
      301 tggcaccggt aatcaaaacc gtctgagttc cgcgctccgc gttcattccg cccccttatg
      361 gcggaggcta tcgtatcgca aatcggaacg agtcgcgttg ggcgtgtcgg cgtctcgttt
      421 ttggcgcagg aggtggagac actgctttct gggccggtga ggatcgctgg actaatttca
      481 tcgcttacga ttcaataaca acgcctaggt gagcgtgcat gccggctgac gaagacctct
      541 gcatccctgc gcccgaaaaa aagtggcggc tcggcttttg gagcctgatc gccacgcagt
      601 ttcagggcgc cttcaacgag aatgggctga aatttctcgt tatctacctc attcttgcgg
      661 tcgaaaaaga caaggcacaa cgtgaccagc tcgaattgct ggtcggcgtg ctcttcgcgg
      721 cccctttcat tctcttttcg ctgacgggcg gatatctcgc ggaccgtttc agcaaacgcg
      781 ccgtcaccat ctggacgaaa gtgtttgaag tgggcgtcat gctgttcgca gcagcagccc
      841 tcgtgggacc caatctgaaa ttggcgctgg cggcgatctt tctcgtgtgc acgcagggag
      901 cactgtttgg gccgtccaag tacggtttgc tgccggaact gcttccccag gaaaggcttt
      961 cgtggggcaa cggcgtcctc gaactcggga ctttcgttgc catcatcatt gggagcgtga
     1021 gtggttcttt tctcgccgag gctttcgacg ggcgcgaggt ctattcgggg gcgctgctgc
     1081 tcggttttac gattgtcggt ttggcctgga gctttggaat cagccgcgtg cccgcagcgg
     1141 accccgcgaa gaggttccat ctcacctcgc tgatcgatgt ctggtcgccg atgaaagtga
     1201 ttcgcggcga ccgcgtgctg tggctggcgg tgctgggcaa cacctacttc tttttcgtcg
     1261 gcgggctgct gcaattcaac atttttattt atgggcaaga cgtattgcat atccgttcga
     1321 cacagggagg tttactgcaa gcggcagtgg ccatcggaat cggacttggc agttttgccg
     1381 cgggatatct ctcgggcgga aaaatagagt acggactgat tcctctcggc tcactgggca
     1441 tgaccgccct cgggctctgc ctagcgatcc cggacttgtc gttccgcgcc gtgctactgc
     1501 tgctggcggc actgggcttc ttcgggggat tttttattgt cccgatcagc gccctgatcc
     1561 agcaccagcc ggaggaagac aagaagggcg tcgtcatcgg cacggcaaac tggctttcct
     1621 ttgtgggcat aggcgccgct tcaggtatgt attacgctgc aactcatttt gcccatctca
     1681 gcccgggcgg aattttcttc tggtcggcgt ttgcgacact cggagcaacg gcctacgtgc
     1741 tttggctgct gccggattcg ttgctgcgct tgatactgtg gttcgccacg cacacgctgt
     1801 accggttgga tgtcgaaggg cgggaaaaca ttccggcgcg tggcggagcg ctgctgactc
     1861 ccaatcacgt ctccatggcc gacgccgtgc tgctcatcgc gtcgatcgat cgcccgatcc
     1921 ggtttctgat gttcaagggt tcttacgaac acccgctggt caaacccttc gcaaagatca
     1981 tgggcgtgat tcctattgcg ccggatcaag gtccgcgcga aatgatccat tccttgcggc
     2041 tggccacgga cgcgctgaaa aacggcgaag tcgtttgcat ctttccggaa gggcagatga
     2101 cgcgcatcgg ccagatgctg ccgttccgcc gagggatgga acgcatcatc aagggcgtcg
     2161 acgttcccat cattcccgta aatctggacg gcgtgtgggg ctcgatcttc agttttgccg
     2221 gtgggcgatt tctttggaaa ttcccgcggc aaattcctta ccctgtgcga gtcacatttg
     2281 gaaaaccgct gccttcgacg gcgacagcgc aggacgtgcg ccgtgccgtg caggacctag
     2341 gcgcagaagc ctttgcgcgc cgcaagaagc gcatgcacac gttgccccaa tcatttattt
     2401 actcggcgcg caggcatccc ttccgctttg ccatggccga cgggcagaga ccgaaactga
     2461 gctggttttc agcgttggtg ggggctctgg tcctcgcgcg gcgcttaaga aagcaatggc
     2521 agggccagga aatggtcggc atcctgctgc cgccatcggt tcccggcgcg ctcgtgaatt
     2581 acgccgccat gttgctgggc aaggtgccgg tgaacctgaa ttacaccgtt tcgaatgaaa
     2641 cgctggcttc ctgcgcgcaa cagtgcaact tgaaaacgat cgtcaccgcg cgggtctttc
     2701 tggaaaaggt caaaatccag ccacccggcg aaacaatttt catcgaggac gtcgtcaagg
     2761 ccgccggctt cggcgaacgg gtcgccgccg cgttagtagc aagcttcttg ccggcaaagg
     2821 ccgtcgcgaa atttgcgggt tgcgatcgcc ccgcgtcgtt ggacgacatc gccacgatta
     2881 ttttttcaag cggctcgacc ggcgacccca agggcgtcat cctcacgcac tacaatattg
     2941 cttcaaacgt ggagcagctc aaccaggttt tcatgctgca cgctgaagac cgcatcatgg
     3001 gcatccttcc gtttttccac tcgttcggtt ttacgggaac gctttgcctg cccgccgcca
     3061 ccggcatcgg cgtcgttttt catcctaatc cgttggattc gcgcgtcatc ggcgctcttg
     3121 tcaacaagta cgcggtcacc atgcttctcg cgacgccgac atttctgaat gcctacacgc
     3181 gccgctgcac gccggaagat tttggcagct tgcgcttcgt tatggctggc gcggaaaaat
     3241 tgcccgaccg catttcacaa gcttttgaag atcagttcgg tattcgcccg cacgaaggtt
     3301 acggctgcac ggaatgttcg ccggcagtta ctgtgaatac gatcgatttt cgcgccgcgt
     3361 cgttcctcca ggtgggggca aagcgcggca gcatcggcca tcccttgccg ggaataacgg
     3421 tgaaaatcgt ggaccccgac acgttgcagc ctgtgggggt ggatgagccg ggactgctgc
     3481 tcgtgcgcgg cccgaatatc atgctcggct acctcaacaa gccggagaaa accgccgagg
     3541 ttctccgcga cggctggtac aacaccggag acgtcgcg
//
LOCUS       DASYVO010000084         6848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_256625, whole genome shotgun sequence.
ACCESSION   DASYVO010000084 DASYVO010000000
VERSION     DASYVO010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6848
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256625"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /gene="hemG"
                     /locus_tag="VGR03_02660"
     CDS             <1..826
                     /gene="hemG"
                     /locus_tag="VGR03_02660"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEV1993210.1"
                     /translation="EKASGSIVRGMLRLAKSKKGPRERPALQTFREGNETLVRALANK
                     LGPALLTETKVAEISRQNDGSFDIRLENRNAGESVSTKSLILATPTDVTAKLLSPLDA
                     SFESLLNPIEYAAVAVVSLGYRKQDVGHSLNGFGFLVPRSARLRVLGSVWNSSLFPGR
                     APEGHALLTSFVGGATDPAATKLKPEELAGLVHREISALLTMKNEPVFSNVTISARAL
                     PQYNLGHGDRLAAIAKVCSHFPGIWLIGNYLRGPAIGSCVDQAFAVAEEIRKNLSA"
     gene            complement(990..1505)
                     /locus_tag="VGR03_02665"
     CDS             complement(990..1505)
                     /locus_tag="VGR03_02665"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEV1993211.1"
                     /translation="MKRKNGSKDRQEKELPGKPVSASRSEMTEIVLPAQTNPLGKLLG
                     GQVMHLVDMAGALAAHRHSNSYVVTASVDYIDFRNPVNLGEIVLLHSQVNRVFRTSME
                     VGVEVYSENAMTGERKHTTTAYVTFVAVDEHTREPKLVPPLILRTAEERRRFRGAAER
                     RKTRLALRYGK"
     gene            1573..1896
                     /locus_tag="VGR03_02670"
     CDS             1573..1896
                     /locus_tag="VGR03_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993212.1"
                     /translation="MTEQKKTRRQLLEDFVAKKPDDSFSRYGLAMECMNGGDPSAADT
                     HFRALLDRNADYIPAYLMYGQLLARESRTGEAKQILSNGIAAAAKKGDQHARSEMEAF
                     LAELP"
     gene            2421..2954
                     /locus_tag="VGR03_02675"
     CDS             2421..2954
                     /locus_tag="VGR03_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993213.1"
                     /translation="MKNAKPSRSRVAPAESSICQFPFADGRQCRILRHKSHPTLCLFH
                     AREEQQFLELDRIGAELQSLSGDFTTFRDLNHVVGKLFKLVAANRIPARNAALLAYLA
                     QLLLYTQKDLKHEQTIAYGYLGWEKIIRDIYVSAGLACPNPAVIPAAALSLSAPPADP
                     STPAPATLASSTPGISR"
     gene            3157..3777
                     /locus_tag="VGR03_02680"
     CDS             3157..3777
                     /locus_tag="VGR03_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEV1993214.1"
                     /translation="MRPERIPLFPLNVVLLPGAELPLHIFEPRYREMVKNCLEEKSEF
                     GMLLSLPKGVARVGCTAEIKAVVQRYEDGRMDILTAGCAPFRVVELFTEDPLLEGHVD
                     YLEDRESPANSRIQRELVELFEACHTLIFDDYPKNLEAPATEELSYLVAATLPMDLLW
                     KQQILELRSEADRQERLVAYLREWAPHLQKSGAMRQRAGGNGHGLN"
     gene            3820..5328
                     /locus_tag="VGR03_02685"
     CDS             3820..5328
                     /locus_tag="VGR03_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993215.1"
                     /translation="MTASTSMQSSAHSKPRTLGELKRIPDFDPRGASRSVKDELRRNL
                     LRAIEKGETLFPGVHGYEDTVVPQIVNALLSRHNFILLGLRGQAKSRILRGLINLLDP
                     EMPVVAGCEINDDPLKPICRACRERIAADGDNTPISWMSRESRFVEKLATPDVTMADI
                     IGDVDPIRAARGGRDLSDELTIHYGLLPRANRGIFAINELPDLAGKIQVGLFNIMQEG
                     DIQIKGYPLRLPLDVLLVFTANPEDYTARGKIITPLKDRIGAEIRTHYPASVEEGMTI
                     TAQEAWVGRDSGKKIDVPHYLRQVVEEIAFQAREDKRVDRRSGVSQRLPITTLESLVS
                     SAEQRAARNGEKSVVARVADVYAALPAITGKMELEYEGELKGADTIARELIRTAVGRV
                     FSKNFTDINFQPVIQWFELGGELKLPDLATTTERYQQLSKIQGVMEHVGRLGVQDRKD
                     TGAAAAAAEMILEGLWAHRRIGRSEERGFYAEKPKQPDQREGPPRPPRRQFN"
     gene            5570..6847
                     /locus_tag="VGR03_02690"
     CDS             5570..6847
                     /locus_tag="VGR03_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV1993216.1"
                     /translation="MKFIRYGKYIGEPADAIDLQELIKRLGDFFLQSGFESQFYGVSQ
                     MDPERSMEALREAILRALQQGDLLPENAMSDELREMLQHPGAMNSQAVKDLLDKITER
                     LANEGFINPQQPPQVTPPPQTSARGQLGQPHERQSEARFEITDKTIDFLGFKTLQDLL
                     GSLGRSSFGRHDTRDLSTGVESGGVSRPYEFGDTLNMDISATLFNAVRREGVKVPIEM
                     TYSDLMVHQCEYQSSCATVVLLDCSHSMILYGEDRFTPAKRVAMALSHLIRTQYPGDS
                     LNLVLFHDSAEEVPLSELARVQVGPYYTNTREGLRMAQRILLRQKKDMRQIVMITDGK
                     PSALTLEDGRIYKNAFGLDPFVVGQTLEEVNKCKRSGIMINTFMLASDYSLVHFVQKI
                     TEMCRGKAYFTTPYTLGQYLLMDYMSRKTKQIH"
BASE COUNT         1473 a         2366 c         1613 g         1396 t
ORIGIN      
        1 cgaaaaggct tcaggcagta ttgttcgcgg catgctacgc ctcgcaaaat ccaagaaagg
       61 ccctcgggag cgtcccgccc tgcaaacctt tcgcgaaggc aacgaaacgc tcgtccgcgc
      121 gctggcgaat aaactcggcc ccgcgctgct taccgaaaca aaagttgcag agatctctcg
      181 ccaaaatgac ggctcgttcg atatccgtct ggaaaaccgc aacgcaggcg aatctgtctc
      241 caccaagtcc ttaattttgg ctacgcccac ggacgtcacc gcgaagcttc tttctcctct
      301 cgatgcgtca tttgaatctc tgctgaatcc catcgaatac gccgccgtcg ccgtagtctc
      361 tctgggttat cgcaaacaag atgtcgggca ctccctgaac ggctttggct tcctcgtgcc
      421 tcgctccgcc aggctccgcg tcctcggctc cgtctggaac tcatcgcttt tccccggccg
      481 cgccccggaa ggacatgctc tgctgacaag cttcgtcggc ggagcaaccg acccagccgc
      541 aacaaagttg aagccggaag aactggcagg tctcgtacac cgcgagatct ccgctctcct
      601 caccatgaaa aacgaacccg tcttttcgaa tgtcacgatt tcggcgcgcg ccctgccgca
      661 gtacaatctc ggtcacggcg accgcttggc ggcaatcgca aaggtctgct ctcacttccc
      721 gggcatctgg ttgatcggca attatctgcg cgggcctgcc atcggatcat gcgtcgatca
      781 ggcctttgcg gtagctgaag aaatacgcaa gaatctctcc gcataaggct tctgcctcat
      841 aaggtcgtcc cacaattaat tttcggtttc gagtcacctc ggcacgactc ttgtttttcc
      901 aacccgtttt ttcctgctat aaaaaaggag agaataacgt tgccggcctc tcttcttaag
      961 taaaacgaaa gacttaaacc tagaacgaac tactttccat accgcaacgc caggcgtgtc
     1021 ttgcgccgtt ccgccgctcc tcggaaccgc cggcgttcct ccgccgtcct caagattagc
     1081 ggcggaacga gtttcggctc cctcgtatgt tcgtcaaccg ccacaaacgt tacgtacgcc
     1141 gtagtcgtat gcttccgttc tccagtcatc gcattctctg aataaacctc gacgcccacc
     1201 tccatcgacg tgcggaacac ccggttcacc tgggaatgca gcaaaacgat ttcgccgaga
     1261 ttcacggggt ttcgaaaatc gatgtaatcc accgacgctg tgaccacata gctgttcgaa
     1321 tgacggtgcg ccgccagcgc ccccgccata tccacgaggt gcatcacctg tccgccaaga
     1381 agttttccca gtggattcgt ctgcgccggc aagacgattt ctgtcatctc tgaacgtgat
     1441 gcgctcaccg gcttccccgg cagctccttc tcttgtcgat ccttgcttcc gtttttccgc
     1501 ttcatgcgta cccctcgctc cggcgcgatc ttggatgctg ctgccgctac ccgtataata
     1561 cgagacgacc caatgacaga acaaaagaaa acccgccgcc agttgctgga agactttgtc
     1621 gccaagaagc ccgatgattc cttctctcgc tacggcttgg ccatggagtg catgaacggt
     1681 ggcgacccct cagccgccga cacccatttt cgcgcgctcc tcgaccgcaa tgccgattac
     1741 atccccgcct atctgatgta cggccagctc ctcgcccgcg aatcccgcac cggcgaagcc
     1801 aagcaaatcc tgtcaaacgg catcgccgcc gccgcaaaaa agggcgacca acacgcccgc
     1861 tccgaaatgg aagccttcct agccgaactg ccctgacgac tccagctact ccttttctaa
     1921 ctccccgcaa tttactcttg acttagctcc cgcgggggtt ataatagtct caggtacaat
     1981 tgggtcccct ctccgtacgc tcctgttttt ctctgaaatc ctgaattctc actctcggat
     2041 tcgtttcttg cctgagcctt tcgcttttcc tgggacattt cgagtttcgt cccattgcac
     2101 caaaatgcag caaaaaactt ggtaagccca ttgcttgcca cacttccgaa aaatgcgccc
     2161 ataactcctt tatttgccac acatccaaaa acacacggtt taaagtcctt tgtttgccac
     2221 acatccgaaa aaaggggcgt gggggcggaa actgttaact agaaatcgcc cacccgatcc
     2281 tgtagctcag tctgtagctc agccctttag ggctgaggcc ttactcttcc cccgcaccat
     2341 tcaacagtca cgggccacgc atcaccagtc tgtccccggc ttcgctgggc acgaaccaca
     2401 atctaaaaat cgaggtcact atgaaaaacg caaaaccatc ccgttccaga gtcgctcccg
     2461 ctgaatcttc tatctgccag ttcccgtttg ccgacggccg ccagtgccgc atcttacgcc
     2521 acaaatccca tcccactctg tgcctcttcc atgcccgcga ggagcagcag ttccttgagc
     2581 tggatcgcat tggcgcggag ttgcaatctc tctctggcga tttcaccact ttcagggatc
     2641 tcaatcacgt cgtaggcaag ctcttcaaac tcgtcgccgc caaccgcatc ccggcccgca
     2701 acgccgccct cctcgcctac ctggcccagc ttcttctgta cacccaaaag gacctcaagc
     2761 acgaacaaac catcgcctac ggctatcttg gctgggaaaa gatcatccgc gacatttacg
     2821 tcagcgccgg ccttgcctgc cccaatcccg cggttattcc agctgctgcc ttatccctct
     2881 ctgcgcctcc ggctgatccg tccacgcctg cccccgcaac gctcgccagt agcacgcccg
     2941 gaatttcgcg ctgaatccgt ttcctaatgc gctgtcaccc aaacaaaacc cggcttcgta
     3001 cccggcgaga ctcgcaggag tgggggaggt gatccgccaa cccagagaca aaaaggcaat
     3061 ttctaaataa agtgcggagc gcgtgacacg gaggcctgaa accgtctgct tggcgtcctc
     3121 cagagcttcc tctggtatca tctctacctg ggacccatgc ggcctgaacg cattccgctg
     3181 tttccgctga acgtcgtcct cctccccggc gcggaactcc ctctgcatat tttcgagccg
     3241 cgataccgcg aaatggtaaa aaactgtctg gaagaaaagt ccgagttcgg catgcttctt
     3301 tcacttccca aaggtgtcgc ccgcgtcggc tgcactgcgg aaatcaaagc ggtcgtgcag
     3361 cgctatgaag acggccgcat ggacattctc actgctggct gcgcaccgtt ccgcgtcgtc
     3421 gaactgttca cggaagaccc cctgctggaa ggccacgtgg attacctgga agaccgcgaa
     3481 tctcctgcca attcgcgcat ccagcgcgaa ctagtggaac tctttgaagc ctgccacact
     3541 ctgatctttg acgactatcc gaagaatctc gaggcccccg ccaccgaaga actttcttat
     3601 ctcgttgccg ccacgctgcc gatggaccta ttgtggaagc agcaaatcct cgagcttcgg
     3661 tcggaggcgg accgccagga gcgcctggta gcgtatcttc gcgagtgggc cccgcatcta
     3721 cagaagtcag gggccatgcg ccaacgcgcc ggcggtaacg gacacggatt gaattagctt
     3781 gtggcgggcc ggcccggtcg gctcgcggaa cggggcccca tgacggcaag cacctcgatg
     3841 caatcttcag cacattccaa accgcgcact ttgggcgagc taaagcgtat tcccgatttt
     3901 gatccgcgcg gcgcttctcg ctctgtcaag gatgagctcc gccgcaatct gctccgcgcc
     3961 atcgaaaaag gcgagacgct cttccccggc gtgcatggct atgaagacac tgtcgtcccg
     4021 caaatcgtca atgccctgct ctctcgccac aatttcattc ttctcggcct tcgcggccag
     4081 gccaagagcc gcattctccg cggcctcatc aacctgctcg atccggaaat gcccgtcgtc
     4141 gcgggctgcg aaatcaacga cgatcccctg aaacccatct gccgtgcctg ccgcgaaaga
     4201 atcgccgccg acggcgacaa tactcccatc tcctggatgt cccgcgagag tcgcttcgtc
     4261 gaaaagctcg ccacccccga tgtaaccatg gccgacatca tcggcgatgt cgatccgatt
     4321 cgcgcggccc gcggcggccg cgacctttcc gacgaactca ccatccacta cggtcttctt
     4381 ccgcgcgcca atcgcggcat cttcgccatc aatgaactgc ctgacctcgc cggaaaaatt
     4441 caggtcggcc tcttcaacat catgcaggag ggcgacatcc agattaaggg ctacccgctc
     4501 cgcctccctc tcgacgtgct cctcgttttc accgcgaatc ccgaagacta caccgcgcgc
     4561 ggaaaaatca tcacgccgct caaagaccgc atcggcgccg agattcgcac gcattatccc
     4621 gcgagcgtcg aagaaggaat gaccattacc gcacaggaag cctgggtcgg acgcgattcc
     4681 ggcaaaaaaa tcgacgtccc gcactatctc cgccaggtcg tcgaagaaat cgccttccag
     4741 gctcgcgagg acaagcgcgt cgaccgccgc tctggcgtca gccagcgtct gcccatcacc
     4801 actctggaaa gccttgtcag cagcgccgag cagcgcgccg ctcgcaacgg cgaaaaatcc
     4861 gtcgtcgccc gcgtcgccga cgtttatgcc gcccttcccg ccatcaccgg caaaatggag
     4921 ctcgaatacg agggcgaact caaaggagcg gacaccatcg cccgtgaact gatccgcacc
     4981 gccgtcggcc gtgttttcag caagaatttc accgacatca actttcagcc tgtcatccaa
     5041 tggtttgaat tgggcggcga actaaagctt cctgacctag ccaccaccac cgagcgctat
     5101 cagcagcttt ctaaaattca aggtgtaatg gagcacgtcg gccgcctcgg cgtccaggat
     5161 cgcaaggaca ccggcgccgc cgcggccgcc gccgaaatga tcctcgaagg tttgtgggcc
     5221 catcgccgca tcggccgcag cgaagaacgc ggcttctacg ccgaaaaacc caaacaacca
     5281 gatcagcgcg aaggaccacc gcgccccccg cgccgccaat tcaattgatg cccaccacgc
     5341 ccattacggt ttgtagggcc cgccttccga ggcgggccgt tcctctcgcc ggattagttg
     5401 taggggcgca gcactgctgc gcccctgctt ggcatgatct gtacctccgt gcaactccgc
     5461 gcgtatcacg agcaatgctg cgttcctcca ccagtcacca ttcacgagcc accagtcacc
     5521 tccctttcaa ctttcaactt tcgactttca acttcttagg gccgcgatca tgaagttcat
     5581 ccgctacggc aaatacatcg gcgaacccgc cgacgccatc gacctccaag agctcatcaa
     5641 acgcctcggc gatttctttc tccaaagcgg ctttgaatcc caattctacg gggtttccca
     5701 gatggacccc gagcgctcca tggaagccct gcgtgaagcc atactccgcg ctctgcaaca
     5761 aggcgacttg ctcccggaaa acgctatgag cgacgaattg cgcgaaatgc tccagcatcc
     5821 cggcgccatg aacagccagg ccgtcaaaga tctcctcgac aaaatcaccg aacgcctcgc
     5881 caacgaaggt ttcatcaatc cgcagcaacc tccccaagtc acgccgccgc cgcagacttc
     5941 cgcccgcggc caactcgggc aaccgcacga acgtcaatct gaagcgcgct tcgagatcac
     6001 cgacaagacc atcgattttc ttggattcaa aactctgcag gacttgctag gctcgctggg
     6061 ccgcagcagc ttcggccgtc acgacacccg cgacctctcc accggcgtgg aatccggcgg
     6121 cgtctcccgc ccctacgaat tcggcgacac cctcaacatg gacatcagcg ctaccctctt
     6181 caacgccgtt cgccgcgaag gcgtcaaggt ccctatcgag atgacctatt cggacctcat
     6241 ggtccaccaa tgcgaatatc aaagctcttg cgccaccgta gtccttctcg attgcagcca
     6301 cagcatgatt ctctacggcg aagaccgttt cactcccgcc aagcgcgtcg ccatggcgct
     6361 ctcgcatctc attcgcacgc agtatccagg tgactcgctc aaccttgtcc tcttccacga
     6421 ctccgccgaa gaagttcccc tcagcgaact cgctcgcgtc caggtcggcc catattacac
     6481 caacacccgc gaaggcttgc gcatggccca gcgcatcctc ctccgtcaga aaaaagacat
     6541 gcgccagatc gtcatgatca ccgacggcaa gccctccgcc ctcaccctcg aagacggccg
     6601 catctacaaa aacgccttcg gcctcgatcc cttcgtcgtc ggccagaccc tcgaagaagt
     6661 gaacaagtgc aagcgctctg gcatcatgat caacaccttc atgctggcca gcgactattc
     6721 cctcgtccac ttcgtccaga aaatcaccga aatgtgccgt ggcaaagcct acttcaccac
     6781 gccctacacc ctcggccaat atctcctcat ggactacatg tcccgcaaaa ccaagcaaat
     6841 ccactaac
//
LOCUS       DASYVO010000085         3886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_45420, whole genome shotgun sequence.
ACCESSION   DASYVO010000085 DASYVO010000000
VERSION     DASYVO010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45420"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VGR03_02695"
     CDS             complement(<1..784)
                     /locus_tag="VGR03_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993217.1"
                     /translation="MRVYLINPNHLSFGVGVITPRWLYVIAAATPRSFGDPILVDETL
                     AQIDSTQIQPGDVVGIGIHSGNALRGYEIGKLARERGAHVIFGGIHTSLYPQEAFERG
                     GAHAVVKGDGDVIWARVLADCANGLLQQVYEGGRIEAEQFLAARWDLMPRDSYMWASV
                     QTVRGCPKHCSFCSVWRTDGQRPRQRASDGVIHEIIELRRMGFRFIALADDNFYPVTL
                     QDLELARRQNAHDKLAQLQAIRDERFELMARLAELPKDMVFYT"
     gene            1308..2099
                     /locus_tag="VGR03_02700"
     CDS             1308..2099
                     /locus_tag="VGR03_02700"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1993218.1"
                     /translation="MTHIEKHSPGSFCWIELATTDQNAARNFYGKLFGWAVNDMPMGP
                     NEFYSMFTLEGRYAAAGYTLRPDQRAQGVPAHWMIYVAVESADAAAARAAQLGGKVLA
                     PAFDVYDAGRMAVIQDPAGAFFCVWQAKKNAGTGIAGVDGTLCWADLSTPDQEAASKF
                     YSGLFGWTIMKEDEDPAHNYFHIMNGEEFIGGIPPASHRDPNIPPHWLAYFTVSNCDD
                     AAAQAKELGAKFLMPPTTFEDVGRFAVVADPQGAAFAIFQAAPRR"
     gene            complement(2108..2314)
                     /locus_tag="VGR03_02705"
     CDS             complement(2108..2314)
                     /locus_tag="VGR03_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEV1993219.1"
                     /translation="MPRGVSDPAPRNPNGGPATLMRFLPRFPKDFPAAIVVVQHMPAT
                     FTAQFSRQLAECTSLRPGESEIDS"
     gene            2412..>3886
                     /locus_tag="VGR03_02710"
     CDS             2412..>3886
                     /locus_tag="VGR03_02710"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1993220.1"
                     /translation="MSPVTIENPILNSPFVEPERHFRFSDEGITSEIIHGRRTSSYFV
                     PIPPPKTRGGKPQLVFDTQWTQERIEENKFINQVRERVARWRKDGYLEGSITKTTRFL
                     LNYWKNPERARKLFFCQIEALETAIYLTEAAPKYNDAWIENALRRFNDEANPGLYRIA
                     FKMATGSGKTVVMAMLIAWHTLNKLADPQNALFGDAFLIVTPGITIRDRLRVLLPNDP
                     QNYYQTMDILPADLRPELGKAKVLITNFHAFKPRERTAASKLTKQILAQGDSAAFTET
                     PDQMVRRVCRELGSKKNIIVISDEAHHCYRRKPDGEDEKLSGDERREAQKREEEARIW
                     ISGLEAVQKKIGLRANYDLSATPFFLRGSGYSEGTLFPWVVSDFSLIDAIESGIVKVP
                     RVPVADDSVTQGQPTYRDLWLRIRENLPRKGRSTQDLGKVEPKLPAELEGALQSLYSN
                     YEKYYRKWEQNTEARARGLTPPVFIVVCNNTNVSNLVFRYVS"
BASE COUNT          861 a         1198 c         1078 g          749 t
ORIGIN      
        1 gcgtgtagaa caccatgtcc ttgggcagct cggccaaacg cgccatcagt tcaaatcgtt
       61 cgtcacgtat ggcctgcagt tgagcaagtt tgtcgtgggc attttgacgc cgggccaact
      121 ccagatcttg cagcgtgacc ggatagaagt tgtcatcggc cagcgcgatg aatcgaaatc
      181 ccatgcggcg caattcgata atttcgtgga tgaccccatc ggaggcacgc tggcgaggcc
      241 gctggccgtc ggtgcgccac acggaacaaa aggagcagtg cttgggacat ccacgcacgg
      301 tctgaaccga agcccacatg tagctgtcgc gcggcatcaa atcccaacgc gcggcgagga
      361 actgctcggc ttcgatgcgc cctccttcgt agacttgttg cagcagtcca ttcgcgcaat
      421 cggctaaaac ccttgcccaa ataacatctc catccccctt taccaccgca tgagcgcctc
      481 cgcgctcaaa cgcttcttgc gggtagaggc tggtgtgaat gccgccgaag attacatgcg
      541 ctccgcgctc tcgggccagt ttcccgattt cgtagccgcg cagggcattg ccgctatgga
      601 tgccgatgcc gaccacgtcg cccggttgaa tctgcgtgct gtcgatttgc gcaagcgttt
      661 catcgaccag tattgggtcg ccaaaggaac ggggagtagc cgcagcaatt acgtaaagcc
      721 atcgcggcgt aatcacgccg acgccaaacg ataagtgatt aggatttatg agatagacgc
      781 gcactacgcc cgccgccccg gagcatgggc gagcgggcga gcgccccgac aatgattggc
      841 agactgaggc atctacttga cgaactataa gccgtgccgc ggccggtgtc aacgcagtaa
      901 gccagccttc gttctgtttc gcctggtctt cgtcacagcg tcaaaacgat ttgtttccga
      961 tgtgatggca gtccgaacgc tccagccttc aatctacagg ctggcgctgg ccgattgcgc
     1021 ggctggcaga agccggttcg tccgtccaat gtaacggcaa cttcttgcat tgtacatctc
     1081 cggccctcca cgcagttcct tcgcccggtc ccgatccgag cgctgcgaga aaatcgtgta
     1141 aactttttct ccccttgaac gtctatagga aaggaactac tgctcggcga gcgtcttcac
     1201 gttgccacgc gacccgcaac gatttcggct ttggggcaaa aaggggcgga acttttaccg
     1261 gtgaacttga tattttcctg ccgcccgggg cgcagtatac tcccgccatg acacacatcg
     1321 aaaagcattc gccgggttcc ttttgctgga tcgaactggc caccaccgac cagaacgccg
     1381 ccaggaattt ctatggcaaa ttgttcggat gggccgtcaa cgatatgccc atggggccga
     1441 atgagttcta ttccatgttc acgctggaag ggcgatacgc ggccgcagga tatacgctgc
     1501 ggccggacca acgcgcccag ggcgtgccag cgcactggat gatttacgtc gccgtagaga
     1561 gcgccgatgc tgcggctgct cgcgcggcgc aactgggcgg aaaggtgctg gctccggcgt
     1621 tcgacgttta cgacgcgggg cgcatggcgg tgatccagga tccggcgggg gcctttttct
     1681 gcgtctggca ggcgaaaaag aatgcgggca cgggaatcgc cggggtggat ggcacgctct
     1741 gctgggccga tctcagcacg cccgaccaag aggcagcgag caagttttat tccggtcttt
     1801 tcggctggac gatcatgaag gaagatgagg atcctgctca caactatttt cacatcatga
     1861 acggcgagga gtttattggc ggcatcccgc ccgccagtca tcgcgatcct aacattcccc
     1921 cgcactggct ggcgtacttc acggtttcaa actgtgacga cgcggccgcc caggctaaag
     1981 agctgggcgc aaaatttctg atgccgccga cgacgtttga ggacgtggga aggttcgcgg
     2041 tggtggccga ccctcaggga gccgcgttcg ccatcttcca ggcagcccct cgccgctaaa
     2101 gtaataatta cgaatcgatt tcgctctccc cgggtcgcag ggaagtacat tccgccagtt
     2161 gccggctgaa ctgcgccgta aacgtggccg gcatgtgctg cacgacaacg atggccgcag
     2221 gaaaatcctt cgggaaacgc ggaaggaaac gcatcagcgt ggccggcccg ccgttgggat
     2281 tccttggcgc aggatcggac accccccggg gcacaggcaa ggcacgtgct cccagtttcc
     2341 ggggctcatc gataatgaac cgacctaact tattcagttg atatatccta tcgctcggat
     2401 ggtccagact gatgagccca gtcacaatcg aaaacccaat cctcaattca cctttcgttg
     2461 aaccggagcg gcacttccgc ttctcagacg aagggatcac tagcgaaata attcatgggc
     2521 ggcggacaag ctcctatttc gtccccatcc cgccgccgaa gaccagaggc ggcaagccac
     2581 aactggtctt tgacacccaa tggacgcaag agcgcatcga ggaaaacaag ttcatcaacc
     2641 aggtccgcga gcgcgtcgcg cggtggcgca aagatggata cctcgaagga agcatcacca
     2701 agaccacgcg cttcttgctg aactactgga agaaccccga gcgtgcacgc aagttattct
     2761 tctgccagat cgaagccctc gaaacggcca tctacctcac cgaagccgcg ccgaaataca
     2821 acgacgcctg gattgaaaac gccctgcggc gcttcaacga tgaagccaac cccggcctat
     2881 accgcatcgc cttcaagatg gccaccggca gcggcaagac cgtggtcatg gccatgctca
     2941 tcgcctggca cactctcaat aagctggccg acccgcaaaa cgccctcttt ggcgacgcct
     3001 tcctaatcgt tactcccggc attaccatcc gcgaccgcct gcgcgtactt ctgccgaacg
     3061 atccgcagaa ttattaccag acaatggaca tccttcccgc ggacttgcgg cccgaactgg
     3121 gcaaagccaa ggttctcatc accaacttcc acgctttcaa gccgcgtgag cgcacagcag
     3181 ccagcaaact gacaaaacaa atcctcgcgc agggagactc cgccgccttc accgagaccc
     3241 ccgaccagat ggtgcgccgc gtctgccgcg agctcggcag caagaaaaac atcatcgtca
     3301 taagcgacga ggcccaccac tgttaccggc gcaagccgga cggtgaagac gaaaagcttt
     3361 ccggtgacga gcggcgggag gcgcaaaagc gggaagaaga agcccgcatc tggatttcgg
     3421 gactcgaagc cgtccagaaa aagatcggcc tgcgcgcaaa ctacgacctc tccgccacgc
     3481 cattttttct gcgcggttca ggctactctg aaggaacgct ctttccgtgg gtcgtttccg
     3541 acttctcctt gattgacgcc atcgagtccg ggatcgttaa ggtgccgagg gtgcccgtcg
     3601 ccgacgattc cgtcacccaa ggacagccta cctaccgcga tctctggctc cgcattcgcg
     3661 agaatctgcc acgcaagggg cgcagtactc aggacctggg aaaagtcgaa ccaaagcttc
     3721 cggccgaact tgaaggtgcg ctgcaaagcc tgtacagcaa ctatgaaaag tattaccgca
     3781 agtgggaaca aaacaccgag gcgcgggcac gcggtctaac cccgcctgtc tttatcgttg
     3841 tctgcaacaa cacaaacgtc tcgaacctcg tgtttcgcta cgtttc
//
LOCUS       DASYVO010000086         4830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_86934, whole genome shotgun sequence.
ACCESSION   DASYVO010000086 DASYVO010000000
VERSION     DASYVO010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86934"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /gene="hemH"
                     /locus_tag="VGR03_02715"
     CDS             complement(<1..891)
                     /gene="hemH"
                     /locus_tag="VGR03_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV1993221.1"
                     /translation="MAGKKRVGIVLFQLGGPDSLEAVEPFLLNLFLDPDIIPLGPFGL
                     LRRPIAKLISSRRSIPVAGRYAQIGRRSPIGTLTERQRVRLVEAVSPYIDPVAVTAMR
                     YWHPFTTTAVDRLRKTESLDEIVLLPLYPHYSYATTLSSLKEWRRVADQAMWEQPAGR
                     PPERTVNNFHDHPLYIQALVQRIGSVLRQFPDSWRIHLVFSAHGLPMSLVEKGDPYPK
                     QIEETVRLAYELGAKRYPGWPRTHLLCYQSRVGPAKWLQPSLTGTIERLGHEGVKEML
                     VVPISFVTEHIETLHEINIEA"
     gene            complement(893..1036)
                     /locus_tag="VGR03_02720"
     CDS             complement(893..1036)
                     /locus_tag="VGR03_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993222.1"
                     /translation="MKNFDSIFAAYVLGWAVFFGFYLSLGKRASSLQADIEQLKKSVN
                     RGK"
     gene            complement(1033..1710)
                     /locus_tag="VGR03_02725"
     CDS             complement(1033..1710)
                     /locus_tag="VGR03_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEV1993223.1"
                     /translation="MAKRAILGLVTLGLVVAAAFASFYVAPMERTMGPIQRIFYFHAA
                     SAWAGMTAFFICFFGNLLYVWRRQPKWDWLGVSAAEVGLAFTTIVLITGPIWAHPVWG
                     IWWTWDWRLTSTFVMWLLYISYLLLRTLIAEPDRRALLSALFGIFAFLDVPLVYFSIR
                     WWRTQHPQPVVLGGAGSGLDPTMRKVFFFSVLAMHALMILLLSERYRLEKLCGEVDDL
                     RREAEAQ"
     gene            complement(1717..2391)
                     /locus_tag="VGR03_02730"
     CDS             complement(1717..2391)
                     /locus_tag="VGR03_02730"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEV1993224.1"
                     /translation="MALLSNTAVLLGKELRTEFRSRELLTTTMVFILTIVVLFSFTFD
                     PSVTESRRFGPGFLWLAFLFAASLMLQPGFLREQTNDTLSALRLSVSDPFAIFLAKLI
                     ANTIFLLLTEVLMLPVFAVLYNVHIFAVFPQLVLVFFFGSVGVAIPGTAFSAISAQAR
                     MRELLLPLLLLPLLTPVLIASTEATAALLAEKPELQGNWLGFLAGFDVVFLTALWLFG
                     EYLLEE"
     gene            complement(2392..3096)
                     /gene="ccmA"
                     /locus_tag="VGR03_02735"
     CDS             complement(2392..3096)
                     /gene="ccmA"
                     /locus_tag="VGR03_02735"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEV1993225.1"
                     /translation="MTPTVFSPTGIHFENIEKRYGGLYALRRVSLEISAGECVVFAGR
                     NGSGKTTLLRIAARLARPSSGTVSFLNSSKERIETLTAAGFVAHATMVYDELTAEENL
                     LLFARLQGIAEPAARAETLLRKVGLEERRNSLVRTFSRGMRQRVAIARALLNEPAVIL
                     LDEPTTGLDPQGASWFAQTLRGLRDSGRTILMSLHGESEVSNLATRAIQLDAGSVVAD
                     TRTGANFRSILAFANA"
     gene            complement(3141..4460)
                     /locus_tag="VGR03_02740"
     CDS             complement(3141..4460)
                     /locus_tag="VGR03_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV1993226.1"
                     /translation="MRRRYSVRLALLGTLVLAPLAIAEAGTVRGTVVNGTTGKPAAGI
                     ELTLVQLQGGMQEVAHSKSGAQGEFTFDNPGLGAQPMLVRAVYHGINFNHAVPPGTST
                     AQVDIYEASKDAKTINVPSHVVIFQPNGATMIVGEEYQIENKSQPPLAFYRSEGSFDF
                     ALPEKGELQQVAAAGPAGMPVVQLPIDKKNNHYSIAFAFRPGESSVRYSYELPYTGNA
                     ATVKIPTIHPGGRLLVVAAPSVQISGDGLVAGGQEQGMNLYSRQDVPAGTLVAVSVAG
                     MAPAPDANAGVEQAQQGGQQGRDVQQGGEASGVSIQQVPGRLDGLKWYLIGGFVCMFA
                     MLAILLARKPVVAVAGPALVEKEVPVAKQRKSKSSPPSAPIAPVTPTNGAASLAEVDA
                     AIGTSLDGLKERLFRLELRRQAGTISEEEYAQERAHAEKVLRDLVRG"
     gene            complement(4457..>4830)
                     /locus_tag="VGR03_02745"
     CDS             complement(4457..>4830)
                     /locus_tag="VGR03_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993227.1"
                     /translation="MLPALYTLAIFEIATGDWAVLGACLALAVATILFIFYIQPDASD
                     LAPHRTKLDQLLERRDTIYDNLRDLRFEYRSGKYSEGDFEAMKTALENEAALVLAEID
                     QVTDAQVRRPRGTRSADGSAQ"
BASE COUNT         1060 a         1467 c         1372 g          931 t
ORIGIN      
        1 ggcctcgatg ttgatttcgt gaagtgtttc gatgtgctcg gttacgaacg aaatgggaac
       61 caccagcatt tctttcacgc cttcatggcc cagccgttcg atcgtaccgg taagcgaggg
      121 ctgcagccat ttcgcggggc ccacgcggct ctggtagcac agcaagtggg tccggggcca
      181 gccagggtac cgcttggccc ccagctcata ggctagacgc accgtctcct cgatctgttt
      241 cgggtaagga tcgcccttct cgaccaggct catcggcaaa ccatgcgcgc tgaataccag
      301 atgaatccgc caactgtccg gaaattgccg caatacggat ccgatccgtt gcaccagcgc
      361 ctgaatatac agtggatgat cgtgaaaatt gttgaccgtg cgttccggcg gtctcccggc
      421 aggttgttcc cacattgctt gatcggcgac ccggcgccat tccttcaagc tgcttagcgt
      481 tgtcgcgtac gaatagtgcg gatagagcgg taagagaacg atttcatcca gcgactcagt
      541 cttgcgcaac ctatcgactg ccgtcgtcgt gaatggatgc cagtagcgca tcgccgtaac
      601 agccacggga tcgatgtagg gcgagacggc ctccaccagc cgcactcgtt gccgttccgt
      661 gagcgttcca attggcgagc gccggccgat ttgagcgtag cgcccggcga cgggaatcga
      721 ccggcgcgag gaaatgagct ttgcaatcgg gcggcgcagc agtccgaaag gcccgagggg
      781 aatgatgtcg gggtccagaa acagattcag cagaaacggc tcgacggcct ccagcgaatc
      841 cggcccgccg agctgaaaca gcacgattcc gactcttttc ttccctgcca agctacttgc
      901 cccggttcac ggatttcttg agttgttcga tgtctgcttg cagtgaggaa gcgcgcttcc
      961 ctagactgag gtagaagcca aaaaagaccg cccatccgag tacgtaagcc gcaaagatac
     1021 tgtcaaagtt tttcattgag cctccgcttc gcgccgcaaa tcatcaactt ctccgcacaa
     1081 tttttccaat cggtaccgct ccgaaaggag caaaatcatc agagcgtgca tcgccagcac
     1141 ggaaaagaag aagactttac gcatagttgg gtcaaggccg gagccggcgc cgcccaacac
     1201 tactggttga gggtgttgcg tccgccacca tcgaattgaa aaatagacga ggggcacgtc
     1261 gagaaacgcg aaaattccaa agagcgcgct gagcagcgcg cggcggtccg gttccgcaat
     1321 aagggtgcgc aaaagcaggt aggaaatata tagcagccac atcacgaaag tagacgtgag
     1381 tcgccaatcc caggtccacc agatacccca taccgggtga gcccaaatgg gtccagtgat
     1441 cagcacaatc gtggtgaagg ccaggccgac ttcagcagct gaaacaccca gccaatccca
     1501 cttcggctgg cgccgccaga cgtaaagcag attgccgaag aagcaaatga aaaaagcggt
     1561 cattccagcc catgcgctcg ctgcgtgaaa ataaaagatt cgctggatgg gccccatggt
     1621 gcgttccatc ggagccacgt aaaaggaggc gaaggctgcc gccacaacca agcctagggt
     1681 tacaagcccg aggatcgcgc gttttgccat tgcagattat tcctccaata gatattcgcc
     1741 gaaaagccat agcgccgtca aaaacaccac gtcgaagccg gccaagaacc cgagccagtt
     1801 cccttgcagt tccggcttct cggccagcag tgctgccgtc gcctccgtgc tggcgatcag
     1861 gacgggtgtc agcagcggca gcagcagcag cggcagcagc agttcccgca tcctcgcttg
     1921 cgccgagatt gccgaaaaag ccgttcctgg aatggccacg cctacgctgc caaaaaagaa
     1981 aacgagaacg agctggggga agacggcaaa aatatggaca ttgtaaagaa ccgcgaagac
     2041 cggcaacatg agcacttcgg tcagcagcag aaaaatcgtg ttggcaatga gctttgcgag
     2101 aaaaatcgca aacggatcgc tcaccgacaa ccgcagggcg ctgagcgtat cgttggtctg
     2161 ctctcgcaga aaacccggct ggagcatcag cgaagccgcg aaaaggaaag ccagccacag
     2221 gaatccgggt ccaaagcggc gagattcggt tacggacgga tcgaaggtga agctaaagag
     2281 caccacaatc gtgagaatga agaccatcgt tgtggtgaga agctcgcgcg agcgaaactc
     2341 cgtgcgaagc tccttgccaa gcagcaccgc cgtattggag agcagcgcca tttacgcgtt
     2401 tgcaaaagcc aggatggagc gaaagttcgc gccggttcgc gtgtcggcga ccaccgatcc
     2461 cgcatccagt tgtatcgctc gcgtcgccag gttggaaact tccgactcgc cgtgcaagct
     2521 catcaagatc gtgcggccgg aatctctcaa gccgcgcagc gtctgcgcaa accagctcgc
     2581 cccctgtgga tcaagcccgg tcgtcggctc gtctaggaga atcacggccg gctcgttcag
     2641 aagcgcgcgc gcaatggcga cgcgctggcg catcccgcgg gaaaacgtac gcaccaggga
     2701 gttccggcgc tcctccaggc caactttccg gagtagcgtc tcggcgcgag cggcgggttc
     2761 cgcgattccc tgcaaccgtg cgaagagcag aaggttctct tccgccgtca attcgtcata
     2821 gaccatcgtg gcgtgagcca caaaacccgc cgctgtaagt gtctcgattc gttccttgct
     2881 ggaatttaga aagctcaccg tgccagacga aggacgcgcg agccgcgccg caattcgtag
     2941 aagcgtggtc ttgccggagc cgttccgtcc ggcgaacact acgcactcac ccgccgagat
     3001 ttccagcgag acgcggcgca atgcgtaaag cccgccgtag cgtttttcaa tattttcgaa
     3061 gtggattcct gtgggagaaa aaaccgttgg cgtcaccgga cgacctcgga gtttggaaga
     3121 tcgcccctgg tcttcaacgc tcagcctcgc acgagatcgc gcaacacttt ttccgcatgg
     3181 gcgcgttcct gtgcatattc ttcctcggag atagtgcccg cttgacggcg cagctcgagc
     3241 cggaacagac gctctttcag cccatccagg ctggtgccga tggcggcatc gacttcggcc
     3301 agactcgccg ccccgttcgt gggagtcacc ggcgcaatcg gcgcagaggg agggctcgat
     3361 tttgatttcc tctgcttcgc gacggggacc tctttctcca ccagtgccgg cccggctacc
     3421 gccacaacag gcttgcgcgc cagcagaatc gccagcatcg cgaacataca aacaaatccg
     3481 ccgattagat accatttcaa tccgtccagc cgtccgggta cctgctggat acttacgccg
     3541 gacgcctctc cgccctgctg cacgtctcgg ccctgttgac ccccttgttg agcttgttcc
     3601 accccggcat ttgcgtccgg tgctggtgcc atgcccgcta cgctcacggc cacaagcgtg
     3661 cctgccggga catcctgacg gctgtagaga ttcatgcctt gttcttgtcc gcccgcaacc
     3721 aagccatcgc cgctgatctg cacgctgggc gccgcaacaa ccagcaaccg gccgcccgga
     3781 tgaatcgtcg ggatcttcac cgtcgctgcg tttcccgtat acggcagctc gtaagaatac
     3841 cgcactgaac tctcacccgg ccgaaacgcg aacgcaatcg agtaatgatt attttttttg
     3901 tcgatcggaa gttgtaccac gggcattccc gcgggaccgg cggcggctac ctgttgcagc
     3961 tcgcccttct ccggcaacgc gaaatcaaaa ctgccttcgc tccggtagaa agccagcggc
     4021 ggctgcgact tgttctcgat ttgatattct tcaccgacaa tcatagtcgc gccgtttggc
     4081 tggaagatca ccacgtgtga aggaacgttt atggtcttgg cgtctttgga cgcttcataa
     4141 atatccacct gcgcggtgga cgtgcccggc ggcacggcgt ggttgaaatt gatgccgtga
     4201 tacaccgctc ggacgagcat cggctgcgca ccgagccccg ggttgtcaaa cgtgaattcc
     4261 ccctgcgcgc cgcttttgga gtgggcgact tcctgcatcc cgccctggag ttggaccagc
     4321 gtgagttcaa ttcccgctgc tggtttgccg gttgtgccgt tcaccaccgt gccgcgaact
     4381 gtccccgcct cggcgattgc cagcggagcc agaacgagcg tgccgagcag agccagccgc
     4441 acggagtaac gtcgcctcat tgcgcgctcc cgtcagccga gcgcgtgccg cgcggacggc
     4501 gcacctgcgc atccgtcacc tgatcgatct ctgcgagcac cagcgctgct tcattctcaa
     4561 gggctgtctt cattgcttcg aagtcacctt ccgagtattt tccagaacga tactcaaaac
     4621 gcaggtcgcg caaattgtcg tagatggtgt cgcggcgctc caatagttga tcgagctttg
     4681 tgcggtgcgg agcaagatcc gaggcatccg gctgaatata aaatatgaat agtatggtgg
     4741 cgacggccag ggccaggcac gcacccaata ccgcccaatc cccggtggca atctcgaata
     4801 tcgcaagtgt gtagagagcg ggaagcattt
//
LOCUS       DASYVO010000087        12562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_241883, whole genome shotgun sequence.
ACCESSION   DASYVO010000087 DASYVO010000000
VERSION     DASYVO010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 12562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12562
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241883"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VGR03_02750"
     CDS             complement(<1..937)
                     /locus_tag="VGR03_02750"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV1993228.1"
                     /translation="MDCPQCHSSNPATALRCAKCATPLPGAGRKVVGQTVATPPPDPD
                     AVARIEKGTSANLKGTPLAGNTSTPEDLESSTLAGQSKRPPPADPNVTLLDEATSASG
                     IASGWAVPATAGSTGVYAASLAALQSGALLGDRYEIIRQLGQGGMGAVYQARDREVDR
                     MVALKVIRPELAGQPEILQRFKQELILARQVTHRNVIRIFDLGEAGGIKFITMEYIVG
                     RDLRSLLIEKGKLPPAEAVAIIEQVSLALEAAHGEGVVHRDLKPQNIVVNGDGKVCVM
                     DFGIARSIELEGMTRTGALVGTPEYMSPEQVQGEKA"
     gene            complement(1590..4646)
                     /locus_tag="VGR03_02755"
     CDS             complement(1590..4646)
                     /locus_tag="VGR03_02755"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEV1993229.1"
                     /translation="MIRPSRWTQVLALLSLGSSLMCATSRAQIPFSAPKNVSNNADFS
                     LTPQVAVDGGGNIYAVWEDDNATNSNNSNILFSRSTDGGVTFSTPINVSKSSGLSFSP
                     RIAVDTNGGVNVVWVDNTPGHQTIFFSRSTDGGVTFSTPVNISNDPSDSASPQIAVDA
                     TGNISVVWENDTINLGVFYAHSTDGGVSFSSPLPLTTNTSGSFGPQLAVDANGNICVV
                     WEDDFSTRSHVSFSRSADKGATFSTPQLNLSKDSTGNSTAPMIALDGGGNINVVWVDD
                     SPGYFAIFFTRSSDNGTIFSTAKGISNTSGNSANPQLAVDASGNVHVVWQHNTPQVFN
                     KDIFFARSADGGATFTNPPLNLSANPGDSANPWLTVDSAGNLNLGWADTTPTIRGNIL
                     FSQSADAGVTFSAPQNLSNDSGGSSDVQIAADKNGNLDVVWSDGTSGMNQILFSRFTN
                     PKKANTPPVANAGPDQTLPATGPGGTSVTLDGSLSSDPDGDALTFVWTEGSTVVGNSA
                     VASLNVTMGVHTYTLTVTDTAGLSSTAITHVTITNTPPVANAGPDQTLPATGPTTAVT
                     LNGSLSSDPDGDALTFVWTQSGTVVGNSAVVPLTLKIGTYTFTLTVTDAGGLSSSAVT
                     HVTITNLPPVANAGADQTLPAAGPGGTSVTLNGSLSSDPDGDALTFVWTEGSTVAGKS
                     AVVSLTASIGTHTYTLTVTDAGGLSSSAVTHVTITARPPVANAGPDQTLPTAGPNGTP
                     VTLNGSQSSDPNGYALTFVWTQGGAVVGKSAVLPLTLNVGAYTFTLTVTDTAGLSSSA
                     VTHVTIAARPPVAIVVADPTLECAGPGGTLAKLDGSASIDPNGYPLKFVWTEDSSGKI
                     VGNSAVVWVMVSVGPLPHSYTLTVTDAITGLSSSAKTSVTVQDTLPPTLGVSLSYSAR
                     RLRNNELVLVNATIHVSDVCDPNPKVKLISITSNDPADRGRHAVRDVEAVGGGPVAFG
                     TDVRSFLLRAERLENRTPLVYKVTYSATDASGNTKTTTALLNLSAR"
     gene            complement(5124..5222)
                     /locus_tag="VGR03_02760"
     tRNA            complement(5124..5222)
                     /locus_tag="VGR03_02760"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5186..5188),aa:Sec,seq:tca)
     gene            complement(5239..7722)
                     /locus_tag="VGR03_02765"
     CDS             complement(5239..7722)
                     /locus_tag="VGR03_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993230.1"
                     /translation="MQMELLSAGQTETARMELKRYDVRIPMGTLAIGVDNIHHDVFLS
                     PKLVQTARDYLFDLIRQSTSATYFAGIELRTVKAPDTATFRKLLTDLMQGSLTLAKSQ
                     KNIEIDCLFRVALLKFLTQEIASQFGNLILEGKEWIRQRGEQFERSQQAHVIKARLLE
                     LQTARRGVIREVGQQIQQMLAEIDEGSMVKGRRALFGDEFSAAYGLLKNRIVFLDGGK
                     DEHFFLENYVLLGNYVHDPDRFEAMDELFQDIVRDAGVSVEQEPGSADANQAYTALLE
                     QAGAVRNEIANLEGQREAVQKKMGRGKGMFSRALSNPERGDFRASLNDIEARLKHEKL
                     KLEELGPQIDVAKQKLDYFHKGPAGRLGDYLNEPENARWMFDASISDDRWAGVRAKRL
                     DQLIASLEQKEILLQVLAGYEIRAVSRDYSPPLHLQQLRKALVYKEELKRVVQVLKQF
                     PARRVSAAPLEELSRRIRRYSREETQALILKFAADFMRLRRDLRDAEHLAACMERINL
                     VTTEKACELSRINNRLYEYVLPEEAPAASDQVISHVIIKADVRGSTQLTQDLLARGLN
                     PASHFSLNLHEPVKKILDRYGAKKVFIEGDAIVLAIFETESNHAYQRAVAKACVLGRQ
                     ILSVCSGYNDRAAASQLPRLEIGLGMAFQGSAPTYWVDGDSRIMISKALNLSDRLSGC
                     AKLARRMLAAQKTHFSLYQFLTVMEGASAEEIEEFLIRYNLNGIELNEEGFAKLADEI
                     SLEIIETKLDMPWGRDTVTLYYGEVPVGETFELIVIRKAFAREILPDGKIGKPSSHAY
                     YEVCTAPALHELVAALTRAQAVRIESVRH"
     gene            complement(7844..8293)
                     /gene="tsaE"
                     /locus_tag="VGR03_02770"
     CDS             complement(7844..8293)
                     /gene="tsaE"
                     /locus_tag="VGR03_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV1993231.1"
                     /translation="MSFYASCSAVDEVFISHSAEETMRWGREFAAGLKAPVLVLLSGE
                     LGSGKTTLTKGIVGGLGVAPEDEVTSPTFTLVHVYGRDHGVYHADLFRVESLADFESL
                     GLEDIFLKPAVVILEWPERFHLKTAWPRVQVRLEHLGGDSRRITVLA"
     gene            complement(8259..9869)
                     /locus_tag="VGR03_02775"
     CDS             complement(8259..9869)
                     /locus_tag="VGR03_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV1993232.1"
                     /translation="MKALTAAEMREVDRLTTERFGIPSLQLMEAAGRHTAEAVLGEFA
                     PEIPSRVVVLCAKGNNGGDGFVAARYLKAAGAAVRLFLFGGPDQVRGDAAENLRRWQE
                     AGGGVSVVKDTAAWEAAWPDVAGAEVIVDALLGTGLRGPAEGLIAQAIGDVNRLSGDA
                     TAPTPRLVLAVDTPSGLPSDGGPAEGPVLRAHQTVTFTAPKIGQLLAAGAAAVGALRV
                     CQIGSPAGVVEEVGQSNIRLAEPLEFAALPLVRAADAHKGSFGHVLLVCGSLGKSGAA
                     VLAGRGALRAGAGLVTVAAPDVVLPGVAAAQAEYMTEPLASTEAGTASLASLSYGRFS
                     RLEEGKSVLGIGPGLGTHQETQQLIRTVVRDTRLPVVLDADGLNAFVGRSEELCGSEV
                     RPIAITPHPGEMARLLGTSSDAVQSNRLRAAQEAARRWNAVVILKGFHTLVAAPGGTV
                     FVNASGNAGLAKGGSGDTLTGVLAALTAQFGTRDWARVLALGVYLHGRAAELAARGTD
                     PSGLLAGEVSDALPAARHELLRELQRRG"
     gene            complement(9921..10664)
                     /locus_tag="VGR03_02780"
     CDS             complement(9921..10664)
                     /locus_tag="VGR03_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1993233.1"
                     /translation="MAIRNRAALITGGSRGIGRGIALRLGQDGARVAIAYRSNKGAAQ
                     QTLRQLQAVGADCVAMETDITVAGNAEQLVQTVVDRFGRLDILVNNVGDFRWGLLAES
                     SAEEWQQIFTSNLWSVFYASRAALPQMRRGRWGRIVNLGAVGAERAFGQAKISAYAAA
                     KAAVVAFSRSVALEEAKNGITVNVVNPSNIDEKELTLSEARRMRDARFPIGRPPTAED
                     VAAAVAFFASEEAEYVTGQVVNVSGGWML"
     gene            complement(10741..>12562)
                     /locus_tag="VGR03_02785"
     CDS             complement(10741..>12562)
                     /locus_tag="VGR03_02785"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEV1993234.1"
                     /translation="YLLFRLIGWSRARLLWSLRNRLIVAYLFIAVVPVLLLLTLAILA
                     AQILYSQLAAYLLYEDLQQRVELIGDTAEHIAAAQKALPPSATEALARQVLTVEARAS
                     HDEDLPGLEVEFSNDASLLRRLAAGENHFVGLVQTGEQLTLLAIRETQTTRGKTIVRL
                     SAPLNAEFLERLAPELGPIQLSLAERVTGTPPAGILYSSGDSRFYIGKSVGTRRRTLQ
                     PPGFWIDPQVGGLSKFDVVYLPGEGAAQRGYPVFALVRARPSQLNHRIFSSVGEFSNL
                     YLVLFALVAVVFLLIEIAALVTGITLTRTITKAVDELYRATQYVSAQDFTHRVRIERR
                     DQLGVLGASFNQMTSSIGSLIEEQRERQRLENEISIAREVQAQLFPQKLPSVPGVEIE
                     AVCRAARVVSGDYYDFIQMGPKHLALVVADISGKGISAALLMASLQAALRSQVLVPGS
                     ELLSTAELVSRLNRHLTRNTADDRFATLFLAVFDSETRKLRYTNAGHLPALCISNGVS
                     ARLEIGGMVLGVVEDYAYEEGSVVVAPGALLIGYSDGLVEPENVYGEQFGIHRLEDAA
                     LRRQSAPVSAVAESLITAADEWAGTPEQADDMTVVVARFG"
BASE COUNT         2474 a         3872 c         3796 g         2420 t
ORIGIN      
        1 cagccttttc gccttgcact tgctcgggtg acatgtattc cggtgtgcct acgagcgcgc
       61 cggtccgggt cattccttca agctcgatcg agcgggcgat gccgaaatcc atcacgcaaa
      121 ctttgccgtc gccgttcacc acgatgttct gcggcttgag gtcgcggtgg acgacgcctt
      181 cgccatgcgc ggcttccagc gccagagaca cctgctcgat gatggccacg gcttcggcgg
      241 gcggcagctt gcctttttcg atcagcagcg accggaggtc gcggccaacg atgtattcca
      301 tagtgatgaa cttgataccg ccggcctcgc ccagatcgaa aatgcggatg acgttgcgat
      361 gcgtcacctg ccgcgcgagg atgagttctt gcttgaagcg ctggagaatt tccggttggc
      421 cggccagttc ggggcggatc accttcagcg ctaccatccg gtcgacctcc cgatcccgcg
      481 cttgatagac cgctcccata ccgccttgcc cgagctgccg aatgatttcg tagcgatcgc
      541 ccaggagcgc gccagactga agcgcggcga gggacgcggc gtacactcca gtgctgccgg
      601 ccgtcgcggg caccgcccag ccggaggcga tgccgcttgc gctggtagct tcgtcaagga
      661 gcgtgacgtt cgggtctgca ggcggtggac gcttggattg tcccgccagg gtcgaactct
      721 ctagatcttc tggtgtggac gtgttcccgg ccaacggtgt gcctttgagg ttcgccgacg
      781 tgcccttctc gatgcgcgcg acagcgtcgg ggtctggcgg gggcgtggcc acggtctggc
      841 cgacaacttt tcggccagcg ccgggcagcg gcgtagcaca tttcgcgcaa cgcaaggccg
      901 tggcgggatt gctagagtga cactgagggc agtccatgct tcgcgccgac caggcgtcag
      961 aaccgcccca ttatggacca acgcgcggcg aaagcaacac cctcgcagag acctctgcgt
     1021 tcgtcagcaa cttttttgcg tatttcagcg acgcagtcgc gtaagggcta tagagtcatc
     1081 tagacttatg gagcgggcga tgggaatcga attgcatccc aagtttctaa gcctcaccgg
     1141 aacaaggtgt taccagccgt tctgtgagtc aattgttgcc aaatgttgcc aaaagtaagc
     1201 agccgtcaat cggcccggct tgcgagggtg aaggctccgc tgctctgtgg caggtgatag
     1261 tagaagtagt tcccactcaa ttgccaattc tcaaacttta agcgattttg atagcctcta
     1321 aacatgcagc gtcataaacc cgcgaaggca ttcgggtacc tgcgcgttgg actctaagtc
     1381 gaaccggttc gattcagcct tttatgtggg tagaaatatt acaagtcgag aatctacccg
     1441 gggccaggct gtattggagg gagtgcgtgc gtccgcgcgg tttagtcgat ggaaacttcg
     1501 agcgaatcgt aacaacacaa cgacgggagc gcggttctct gtcgcgctcc cgttgaccgc
     1561 cgaaccgtat ccgctaccaa ccttcttact taacgcgcag acaggttcag cagtgctgta
     1621 gtcgtcttgg tgttgccaga agcatccgta gccgagtacg tgaccttgta gacgagtggc
     1681 gtacggttct cgagacgttc cgcgcgaagc aggaacgacc tcacgtcagt gccgaaggca
     1741 acgggcccgc cgccgacagc ctcaacgtcg cggacggcgt ggcggcctcg gtcggccgga
     1801 tcattgctgg tgatcgagat caacttgacc ttgggattgg gatcgcagac gtcactgaca
     1861 tggatcgtgg cgttgaccag gaccagttca ttgtttctca gtcggcgcgc gctataagac
     1921 aatgacacgc ccaacgtggg cggcaacgtg tcctgcaccg tcacgctagt tttcgccgaa
     1981 gaagaaagac ctgttatcgc atccgtgaca gtcagcgtgt aggaatgggg gagggggccc
     2041 acgcttacca tcacccatac gactgcagag ttgcccacga tcttgccgga gctatcctcg
     2101 gtccacacga acttgagtgg gtatccattc gggtcgatgg acgctgaacc atcgagcttg
     2161 gccaatgtgc ctcccggacc agcacattcc agggttgggt cggctaccac gattgcaacg
     2221 ggtggccggg cggcgatggt tacgtgggtg acggccgagg aggacagacc tgctgtgtcc
     2281 gtcacagtca gcgtgaatgt gtacgcgccg acgttcaggg tcaagggcag aaccgcagac
     2341 ttgcccacga cagcaccgcc ctgagtccag acgaaggtaa gtgcgtatcc attaggatcg
     2401 ctggactgcg aaccgttcag cgtgacagga gtgccgttcg gacccgcggt tggcaaagtt
     2461 tgatcgggtc cggcgtttgc gaccggtggc cgggccgtga tcgtaacgtg ggtgacagcg
     2521 gaggacgaaa ggcctcccgc atccgtgacg gtcagcgtgt aggtgtgcgt gccgatgctc
     2581 gcagtcaatg acacaaccgc agacttgccc gcaaccgtgc tgccctccgt ccagacgaag
     2641 gtaagtgcgt cgccgtcagg gtcgctggac agcgaaccat tcagcgtgac agaagtgcct
     2701 cccggacccg cagctggcag agtttggtcg gctccagcgt ttgccacagg cggcagattg
     2761 gtgatggtta cgtgggtgac ggccgaggac gaaaggcctc ccgcatccgt gaccgtcagc
     2821 gtgaatgtgt acgtgccgat tttcagggtc agcggcacaa ccgcggagtt gcccacgacg
     2881 gtgccgctct gtgtccagac gaaagtaagg gcgtcgccat cagggtcgct ggacagtgaa
     2941 ccattcagcg tgacagccgt agtcggaccc gtagccggca aggtctggtc ggggccggca
     3001 ttggcgaccg gcggcgtgtt ggtaatggtt acgtgggtga tggccgtgga ggagagacct
     3061 gcggtgtccg tcacggtcag cgtgtacgtg tgcacgccca tggtcacgtt caacgacgca
     3121 accgcagagt tgcccacaac agtgctgccc tcggtccaga cgaaggtcag tgcgtcacca
     3181 tccgggtcgc tggacagcga accatccagg gtgacagaag tgccccccgg acccgtagct
     3241 ggcagggttt ggtcggggcc ggcatttgcg actggcggtg tgttcgcctt ctttggattg
     3301 gtgaaacggc tgaagaggat ttgattcatg ccggacgtac cgtcggacca gaccacgtcg
     3361 agattgccgt tcttatcggc ggcaatctgc acatcggaag aaccacccga gtcgttcgag
     3421 agattctgcg gcgcggagaa tgtcacaccg gcatcggcgg attgagaaaa gagaatattg
     3481 ccgcgaatcg tcggcgtggt gtcagcccaa cccagattga gattgccggc ggagtccacg
     3541 gtgagccacg gattagccga atcccccgga ttggctgaga gattcaacgg cgggttagtg
     3601 aatgtggcgc ctccatcggc tgaacgagca aagaatatgt ccttattgaa cacctggggc
     3661 gtattgtgct gccaaacaac gtggacattg ccgctggcgt ccaccgcgag ttgaggattg
     3721 gcggagttgc cggaggtgtt ggatatgccc tttgcggttg aaaagattgt gccgttatcc
     3781 gaagagcgcg taaagaaaat ggcgaaatag ccgggactat catccaccca gaccacattg
     3841 atgttgccgc cgccgtccag agcgatcatc ggcgcagtgg agttgccggt ggaatccttg
     3901 gaaagattca gctgcggggt cgaaaaggtt gcgcccttat ccgcggagcg gctgaaggaa
     3961 acgtgggagc gggtgctgaa atcgtcctcc cagaccacgc agatgttccc gttagcgtcg
     4021 accgccagtt gcggtccaaa cgagccgctc gtattcgtag taagaggcag tggcgatgag
     4081 aagctaacgc ctccgtcagt ggagtgggcg tagaaaacac ccaagttgat agtgtcattt
     4141 tcccagacga cgctgatgtt gcccgtggcg tctacggcga tttgcggact cgcagaatcg
     4201 gaaggatcat tggaaatatt caccggcgtt gagaaggtca caccaccgtc ggtagagcgg
     4261 ctgaagaaaa tggtctgatg gccaggagta ttgtccaccc aaaccacgtt gacgccgccg
     4321 ttggtgtcga ccgcaatgcg gggactgaag gagagcccgg aacttttgga gacgtttatc
     4381 ggcgtcgaga acgtgacacc gccgtccgta gagcggctga agaggatgtt agagttgttg
     4441 gagttggtgg cattatcatc ctcccagacc gcgtagatgt tgccgccacc gtccaccgca
     4501 acttgcggag tcaatgagaa atcggcgtta ttggagacat tctttggcgc ggagaagggt
     4561 atttgcgccc tcgaagtggc gcacatcaat gacgagccaa ggctcaaaag ggcgaggacc
     4621 tgggtccaac ggctggggcg aatcatggca gacctccttc tagcgatcag cggctaaaag
     4681 cgggcacctg aatcagcact cttccgaatt caaaaaaaaa ttgcctctgg ccagttctag
     4741 cggggacgtt atggctggct cttacctgac gcgacttgga aggcttgtcc tcggggacca
     4801 aaacggtaca agcctaattc tctggcgtta gtatagcgag atcgagcgaa tgaaaagaga
     4861 aattaaggta ctatagacgc taagtagtaa ataccggtac gtcagtacca aatccgtggt
     4921 gaggcccccc ggcgccagtc cctgcgcggc ctccctaatc aactcattta gaagcagttg
     4981 taatttttcc gggctttgct ggaaagagcc gattcgatca cgaagaaaaa cgctacctcc
     5041 ggtgagccag gcgggaatcg tcgcgttaga aagatgcccg gggaaaaaga cagggcataa
     5101 agaaacgtcc catggcgagc agttggcgga agcgtgtggg agtcgaaccc accattcggg
     5161 ccgcgaagga ccgaatcgcc ggctttgaag accgggagag tcaccggacc cctttcgcct
     5221 ccgcatggat tataggggtc agtggcggac cgattcaatc cgtaccgcct gcgccctcgt
     5281 gagggcggcc accagctcgt gcagcgccgg cgcggtacag acttcgtaat aggcgtgtga
     5341 ggagggttta ccgatcttgc cgtccgggag aatctccctg gcgaatgcct tgcggatcac
     5401 gatcaactcg aacgtctcgc cgacgggcac ttcgccgtag taaagcgtga ccgtatctct
     5461 tccccagggc atgtcgagct ttgtctcgat gatttcgagc gagatttcgt ccgcgagctt
     5521 ggcgaatccc tcctcgttca gctcgattcc attcagattg taccggatca ggaattcctc
     5581 gatctcttcc gcagacgctc cttccatcac ggtcaggaat tgatagagcg agaagtgggt
     5641 cttctgcgcc gcaagcatgc gccgggcaag tttcgcgcat ccggagagcc ggtccgagag
     5701 gttcagagcc ttcgaaatca taatgcgcga atcgccgtcg acccagtacg tcggcgcgga
     5761 tccctgaaag gccatgccaa gtccgatttc cagccgcgga agctggctgg cggctgcgcg
     5821 atcgttatag ccgctgcaga cggacaggat ctgccgtccc agcacgcagg ctttcgcgac
     5881 ggcgcgctgg taggcatggt tcgattcggt ctcgaagatc gccaggacga tggcgtcgcc
     5941 ttcgatgaaa accttttttg ccccgtaacg gtcgaggatc tttttcaccg gctcgtgcag
     6001 gttcaggctg aaatgagagg cgggattcaa tccccgggct agaaggtcct gggtgagctg
     6061 ggttgagccg cgcacatccg ctttgatgat cacgtgggaa ataacctggt cgcttgcggc
     6121 cggggcttcc tccggaagga cgtattcgta caaacggttg ttgatgcgcg aaagctcgca
     6181 tgccttttcg gtggtaacga ggttgatccg ctccatgcag gccgcaaggt gctccgcatc
     6241 cctcaaatca cggcgcaagc gcatgaaatc ggccgcaaac ttcagaatca acgcctgcgt
     6301 ttcctcgcgt gaatagcgcc ggattcgccg ggagagctct tcgagaggtg cggcggacac
     6361 gcgccgcgcc gggaattgct tcaggacttg cacgacccgt ttcagctctt ccttgtaaac
     6421 cagcgctttg cggagctgct gaagatgcaa gggcgggctg tagtcgcggc tgacggcgcg
     6481 aatctcataa ccggccagga cctgcagaag aatctctttt tgttccaggc ttgcgatgag
     6541 ctgatccagc cgcttcgcgc gcacgccggc ccaccggtca tcagaaatgg atgcgtcgaa
     6601 catccagcgc gcgttctccg gttcattcaa gtaatctccc agacgcccgg cggggccctt
     6661 atggaaatag tcgagcttct gtttggcaac gtctatctgc gggccgagtt cttccagctt
     6721 caacttctca tgcttcagcc gtgcttcgat gtcgttcaac gaagcgcgaa aatcgccgcg
     6781 ctccggattg ctcagcgccc gggaaaacat gccctttccg cggcccattt tcttttgaac
     6841 tgcctcgcgc tggccttcga gattcgcgat ttcgtttcga acggcccccg cctgctcgag
     6901 cagcgccgtg tacgcctggt ttgcatcggc agaacctggt tcctgttcga cactcacccc
     6961 ggcatcccgg acaatatcct ggaaaagctc gtccatcgct tcaaaccgat ccgggtcgtg
     7021 cacgtaattc cccagcagaa cgtagttttc cagaaaaaag tgttcatcct tgccgccatc
     7081 cagaaacacg atgcgattct tgagcaggcc gtaggcggcg gaaaactcgt cgccaaacag
     7141 agcccgccgg cccttcacca tcgagccctc gtcgatctcc gcgagcatct gctgaatctg
     7201 ctgtccgact tcccgaatca cgccccgccg cgccgtctgg agttccaaga ggcgtgcctt
     7261 gatcacgtgc gcctgctggc tgcgttcaaa ttgctcgccg cgctgccgga tccactcctt
     7321 cccttcgagg atcaggtttc cgaactggct ggcaatctcc tgcgtgagaa acttgagcag
     7381 ggcgacgcga aagaggcaat caatctctat gttcttctgc gatttcgcca gggtgagcga
     7441 gccctgcatc aagtcggtga gcagcttgcg gaaagtggcc gtatccggcg ctttcaccgt
     7501 gcgcaattcg attccggcga agtaggtggc gctcgtgctc tgccggatga ggtcaaaaag
     7561 atagtctcgc gccgtctgca cgagcttcgg gctcaggaaa acatcgtggt ggatgttgtc
     7621 cacaccgatc gccagcgtcc ccatcgggat cctgacgtcg tatcttttta gctccatccg
     7681 ggccgtttct gtctgcccgg cagacaacaa ttccatttgc atgaagtccg ctcgaggctc
     7741 cttctcgaac ttagcagtct acgcggggca ggacaacccg ccgcacccaa gccgttaaca
     7801 accgtcccga aacttggggc gctggaagga ggggggaaaa gacctaggcg agaacggtga
     7861 tccggcgtga atcgcccccg agatgctcta gccggacctg cacccggggc cacgccgtct
     7921 tcaggtgaaa cctttccggc cattccagaa tgaccaccgc cggtttcaag aagatgtctt
     7981 ccaagcccag cgattcgaag tccgccaggc tctccacgcg gaaaaggtcg gcgtggtaga
     8041 cgccatggtc gcggccgtag acgtggacca gggtgaacgt cgggctagtg acttcgtcct
     8101 ccggcgctac cccgaggccg ccgacgattc ccttcgtgag ggtcgtcttc ccgcttccca
     8161 attcgccgga gagaagcacc aggaccgggg ccttcagccc cgcggcaaat tcccggcccc
     8221 aacgcattgt ttcttccgct gaatgcgaga tgaacacttc atccacggcg ctgcagctcg
     8281 cgtagaagct catgccgcgc cgcggggaga gcgtccgaga cttcccccgc gaggagaccc
     8341 gacggatcgg tcccgcgcgc ggcgagctcg gccgcccgcc cgtgcaaata gacgccgagg
     8401 gcgagtacgc gagcccagtc cctggttcca aattgtgcgg tcagggcggc aaggactccg
     8461 gtcagcgtgt ctcccgagcc gcccttggcc agcccggcgt tccccgaggc gttcacgaag
     8521 acggtgcccc ccggtgcggc gacaagagtg tggaatcctt tcaggatgac aacggcattc
     8581 cagcgccgcg ccgcttcctg cgccgcccgc aaacgattgc tctgcactgc atcgctggaa
     8641 gtccccagga ggcgcgccat ctcaccggga tgtggcgtta tcgcaatggg ccgaacttca
     8701 ctgccgcaaa gctcttcgct gcgccccacg aaggcattca ggccatcggc gtcgagcaca
     8761 accgggagcc gggtatcccg cacgacggtc cgaatcagtt gttgagtttc ctgatgcgtg
     8821 cccaggcccg gcccaatccc cagcacgctc ttcccttctt ccagccttga gaaacgccca
     8881 taactcaggc tggcaagcga ggcagtcccc gcctcggtcg acgcgagcgg ttccgtcatg
     8941 tattcggctt gagccgccgc aacacccggc agcacaacat cgggggccgc tacggtcacc
     9001 agccccgctc cggcacgcaa cgctccgcgc cccgccagta cggctgcgcc gcttttcccc
     9061 agcgacccgc agaccagcaa gacatgccca aacgacccct tgtgcgcgtc ggccgcccgc
     9121 accagcggca gagccgcgaa ttccaggggt tccgccaaac ggatgttgct ctggccgacc
     9181 tcttcaacca cccccgccgg cgatccaatc tggcagacgc ggagtgcgcc aactgccgcc
     9241 gcacccgcgg cgaggagttg gccgattttc ggcgcggtga acgtcaccgt ttggtgcgcg
     9301 cgaagaactg gcccctcggc aggcccgccg tcggagggaa gtcccgacgg cgtatccacc
     9361 gcgagcacga gccgcggcgt aggtgccgtg gcgtcacctg atagccggtt cacgtcccca
     9421 atcgcctgtg cgatcaagcc ttcggcgggc ccacgcagac cggtgccgag cagagcgtca
     9481 acgataactt cggctcctgc cacgtcgggc caggctgcct cccaggcagc cgtgtccttc
     9541 acaacgctca ccccgccgcc tgcttcctgc cagcggcgca gattctctgc cgcgtcacca
     9601 cgcacctggt ccgggcctcc gaacaggaaa agccgcacag cggccccggc ggctttcaga
     9661 tagcgcgccg caacgaagcc gtcacccccg ttgttgccct tcgcgcacag gaccaccacg
     9721 cgcgatggaa tttcgggcgc aaattcgcca aggacggctt ccgccgtgtg tctgcccgcg
     9781 gcttccatca attgcaggct ggggataccg aagcgttcgg tggtcaggcg atccacctcg
     9841 cgcatttccg ctgcggtaag tgctttcatg aatgaatcac acaggaatac ggtccggcct
     9901 cgcctggcgc ccgcctgggc tcacagcatc catccgccgc tgacgttcac cacctggccg
     9961 gtcacgtact cggcctcctc ggaggcaaaa aacgccaccg cggccgcgac gtcctccgcc
    10021 gtgggcggcc ggccgatcgg aaaccgtgcg tcccgcatcc ggcgggcctc actgagcgtt
    10081 agctccttct cgtcgatgtt cgaggggttg acgacgttta cggtaatgcc gttcttggct
    10141 tcttccagcg ccacagaacg cgagaacgcc accaccgcgg cttttgcggc agcgtaggcg
    10201 gagatctttg cctggccgaa ggcgcgctcc gcgccgacgg cgcctagatt gacgattcgc
    10261 ccccagcgtc cgcggcgcat ctgcggcagc gccgcgcggc tggcataaaa tacgctccac
    10321 agattggaag tgaaaatctg ctgccattct tccgcggaag attccgccag cagcccccag
    10381 cgaaaatccc ccacattgtt cacaagaata tccaggcgtc cgaatcggtc cacaaccgtc
    10441 tggacgagct gttctgcgtt gccggcgacg gtgatgtcgg tttccattgc gacgcaatcg
    10501 gccccaaccg cctggagctg gcgcagcgtt tgctgcgctg cacccttgtt cgagcggtag
    10561 gcgatggcca cgcgagcgcc atcctggcca agccgcagtg cgattccccg cccgatgccg
    10621 cggcttcctc ccgtaatcag cgctgcgcga tttcgaatcg ccatgttctc cgccgtgaac
    10681 gggagagtat agcccaggcc ctcgggcgag cactatccgc cgatcgcggg cccgtgccgt
    10741 ttacccgaac cgcgccacaa ccacggtcat gtcatcagcc tgttcgggag tccccgccca
    10801 ctcgtccgcc gccgtaatca atgactcggc aaccgcgctc accggcgcgg attgtcgccg
    10861 cagagccgcg tcttccaggc ggtgaattcc gaactgctcg ccgtagacgt tttccggctc
    10921 caccaggccg tcgctgtacc caatcagaag cgctccgggc gccacaacaa ccgaaccttc
    10981 ttcgtaagcg tagtcttcca cgactcccag caccatcccg ccaatctcca gccgtgccga
    11041 aacgccgttc gagatgcata gcgccgggag gtgccccgca ttggtataac gcaacttgcg
    11101 ggtctcgctg tcgaacaccg cgaggaaaag cgtcgcgaac cggtcatcgg ccgtatttcg
    11161 cgtcaggtgc cggttcaacc gggagacgag ctccgcggtg ctcaacagtt cgctcccggg
    11221 aacaaggacc tggctgcgca gagccgcttg caagctggcc atcagcagcg ccgctgatat
    11281 tcccttcccg gaaatatcgg ccaccacaag cgccaggtgc tttggaccca tctggataaa
    11341 gtcgtagtag tccccgctca caacgcgcgc tgcgcggcaa accgcttcga tttcaactcc
    11401 cggtaccgaa ggcagcttct gcggaaaaag ctgggcctgc acctcccggg cgatggagat
    11461 ctcgttttcg aggcgttgcc gctcccgctg ctcctcgatc aggctgccga tcgagctggt
    11521 catctggttg aatgacgcgc cgagaactcc cagctggtcg cgccgctcga tgcgcactcg
    11581 gtgcgtgaaa tcctgcgcgg acacgtactg cgtggcgcgg tagagttcgt ccacggcttt
    11641 cgtgatcgtg cgcgtcaggg tgatccctgt caccagcgcc gcgatctcga tcagcaggaa
    11701 taccacggcc acaagcgcga agagcaccag gtagagattg ctgaattcac ccaccgagga
    11761 aaaaattcga tggttcagct gcgacgggcg tgcacggacc agcgcgaaaa cggggtatcc
    11821 gcgctgcgcc gctccctcgc cgggcaaata cacaacgtcg aacttggaca gcccgccgac
    11881 ctgcggatcg atccagaagc ctggtggctg gagggtccgc cgccgcgtgc cgacgctctt
    11941 gccgatatag aaacgcgagt ctcccgagga atagagaatc cccgccggag gcgtgccggt
    12001 gacgcgctcg gccagactca gttggattgg ccccagttca ggagccaatc tttccagaaa
    12061 ttccgcattg agcggcgcgg agagccggac aatggttttg ccgcgggtcg tttgcgtctc
    12121 ccgaatcgcc agcaaggtca gctgttcgcc cgtttgcacc aggcccacga agtgattctc
    12181 gcccgcagcg agacgccgca gcagcgaagc gtcattggaa aactcgactt ccagaccggg
    12241 caaatcttcg tcatgggagg cgcgagcctc caccgtcaac acctgccgcg ccagtgcttc
    12301 cgtagcggag ggcggaagcg ccttctgcgc cgccgcgatg tgttccgcgg tatcgccgat
    12361 cagttcgacg cgttgctgca aatcctcata gaggagataa gcagccagtt gcgagtacag
    12421 aatctgggcc gccagaatgg ccagtgtcag aaggagaagc accggtacga cggcgatgaa
    12481 gagataagcg acaatcagcc ggttccgcag gctccacaga agccgcgcgc gcgaccaccc
    12541 gatcaaccgg aagaggagat ag
//
LOCUS       DASYVO010000088         1787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_30170, whole genome shotgun sequence.
ACCESSION   DASYVO010000088 DASYVO010000000
VERSION     DASYVO010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30170"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1756)
                     /locus_tag="VGR03_02790"
     CDS             complement(<1..1756)
                     /locus_tag="VGR03_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993235.1"
                     /translation="MSEIFVDPKNPAVVYVPSQNMYRSNDGGHTFTAIKGAPGGDDYH
                     TVWIDPTNTQRIMLGVDQGATISLNGGESWSTWYNQPTGQFYRLATDHRFPYWVYGPQ
                     QDSGTAGIASRGNNGQITERDWFPVGPGESGYTIPDPLDPDVIYNAGPGGSVVRLSKI
                     TGQVRDISPAAVSFGSKYRFNWTIPMVFSPQDAHLLYLGTQFLLKTTDAGASWQAASP
                     DLTRMRADEKDSKQVLGTILTIAPSEIKEGFIWVGTDDGNIQLTKDGGVAWQNVTPTG
                     VSEWSTVSIVEASHFDAGTAYAAVNRNSLDDLRPHIFRTRDFGKTWQETVSGIREGDF
                     VRTVREDPVRKGLLYAGTEQGVYVSFDSGDHWQTLRLNMPVVAVHDLAIEQDDLVAAT
                     YGRSFWILDDVTPLRQMEARAASSGAYLLAPRTAIRVRRDENQDTPLPPEVPAGKNPP
                     DGAILNYVLPANSAGDIQLEIYDADEKLVRSFSSASTPKESEETPFVAEYWIGHPQAL
                     SKAAGMHRLVWNLRYADPRAMHPQSPYNYPIAAIVGSTPLPPQGPLALPGKYEVRLKA
                     GGQVVRQALEVKMDPRVVA"
BASE COUNT          350 a          576 c          477 g          384 t
ORIGIN      
        1 ccgccactac ccgcggatcc atcttgactt ccagcgcctg gcgaacaact tggccccccg
       61 ctttcagccg cacctcatac ttccccggca gcgcgagtgg tccttgcggc ggcagcggcg
      121 tggagcccac aatcgccgcg atcggatagt tgtacggtga ctgaggatgc atggcgcgcg
      181 gatcggcgta gcgcaaattc caaactaagc ggtgcattcc cgctgctttc gaaagcgctt
      241 ggggatgtcc gatccagtac tccgccacga agggagtttc ctctgactct ttcggcgtag
      301 acgcgctgga gaaactccgc accagttttt catccgcgtc gtaaatttcc agttgaatgt
      361 ctccggcaga attcgccggc aaaacgtagt tcagaatggc gccatcggga ggattctttc
      421 ccgcaggaac ttccggcggc agcggcgtgt cttgattctc atcgcggcgc acgcggatcg
      481 cggttcgcgg cgcaaggaga taagcgcccg aagacgctgc gcgcgcctcc atctgccgaa
      541 gcggcgtgac atcatcgagt atccagaacg agcggccata cgttgcggcc accaggtcgt
      601 cctgctcgat ggccagatcg tgcacggcta ctaccggcat gttcagccgg agtgtctgcc
      661 agtgatctcc gctgtcaaac gaaacgtaaa ctccctgttc ggtccccgcg tacagcaacc
      721 ccttgcgcac cgggtcctcg cgcaccgtgc ggacaaaatc gccctcgcga attccgctga
      781 ccgtttcctg ccaggtcttt ccaaagtcgc gcgtccgaaa aatgtgcggg cgcaaatcat
      841 cgagactgtt tcgattgacc gccgcataag cggtaccggc atcgaaatgc gacgcttcga
      901 caatactgac cgtgctccac tccgacacac ccgtcggagt gacgttttgc cacgccactc
      961 ctccgtcctt cgtcagttga atgttcccat catccgttcc cacccagatg aagccttctt
     1021 tgatctccga tggcgcgatc gtcaggatcg tgccaagcac ctgtttcgaa tccttttcat
     1081 cggcgcgcat acgcgtcaga tcggggctcg cagcctgcca gcttgctcct gcatccgtcg
     1141 ttttcaagag aaactgcgtc cccagataaa gcagatgtgc atcctgcggc gaaaaaacca
     1201 tcggaatcgt ccagttgaat cggtatttgc ttccgaaaga aaccgccgca ggggaaatat
     1261 cgcgcacctg ccccgtgatt tttgacaaac gcacgacgct gccgcccgga cccgcgttgt
     1321 agatcacatc cgggtccagc gggtcgggaa tggtgtaacc actctccccc ggtccgacgg
     1381 gaaaccaatc gcgctcggta atctgtccgt tatttccgcg gctggcgatt cccgccgttc
     1441 cgctgtcctg ctgcggtcca tacacccagt agggaaaccg gtgatctgtc gcgagccggt
     1501 aaaattgtcc cgtcggttgg ttgtaccaag tgctccagga ctcgccccca ttcagactga
     1561 tcgtcgcgcc ttgatccacg cccagcatga tgcgctgtgt atttgtcgga tcgatccaca
     1621 cggtgtgata atcatcgccg ccgggtgcgc ccttgatcgc cgtgaacgta tgcccgccat
     1681 cattcgaacg gtacatattt tgcgagggca cgtagaccac cgcgggattt ttcgggtcca
     1741 caaaaatctc gctcatatac caatatccct ggatgcgccg atcctcc
//
LOCUS       DASYVO010000089         5419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_1649, whole genome shotgun sequence.
ACCESSION   DASYVO010000089 DASYVO010000000
VERSION     DASYVO010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="VGR03_02795"
     CDS             <1..1066
                     /locus_tag="VGR03_02795"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEV1993236.1"
                     /translation="SDDVILGCAMPEGEQGMNVARIASLRAGLPVECSAMTINRFCSS
                     GLQAIALAAERIRSGAADVIIAGGTESMSMVPMGGNKVSPNPWLVDNSPDTYISMGLG
                     TEGIARKYGITREQADQFSLASHQKALAAIAAGKFKDEITPVEVSITSWPNGNGDGAP
                     RPKTTPKPSTQSFVFDTDEGPRADTSLDALAKLKPAFHVKGTVTAGNSSQMSDGAAAA
                     VVMSEARAKSLGVKPLARFVSYATAGCLPEEFGIGPVNAIPKALKLAGLKLEDVAVIE
                     LNEAFAAQSLAVIQLAGLDPSRVNPNGGAVALGHPLGCTGAKLTASILRELARRNARF
                     GLVTMCIGGGMGAAGILERL"
     gene            2329..3042
                     /locus_tag="VGR03_02800"
     CDS             2329..3042
                     /locus_tag="VGR03_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993237.1"
                     /translation="MKLLCFAALFLLSFALSAQAPAPVPLKSEPHHHLILENEFVRAF
                     YVEVAPHDATLLHQHDADYVFVVLGPGDFVNAVVGKPEVHATLDDASVRFSRGGFAHI
                     ARTDAGVPFRNITVEFTRPQGALRNLCQKVTDGAVNNCPDSPSPSMKPLFETDEIVVS
                     SGALDAKSKHTEAAPHTAALILALENSNLDVNVGGDVKAQLRSGQVFWLPAGRKHTIS
                     SRGGACRFVLVSFKDSAAH"
     gene            complement(3168..4223)
                     /locus_tag="VGR03_02805"
     CDS             complement(3168..4223)
                     /locus_tag="VGR03_02805"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1993238.1"
                     /translation="MSGDGSEKPVEHRGPMVRLERKQRITESTEREMSHRRKAKGRAM
                     RIRMMVACGVVLMSLWAVGVPARQQQGDKPAAQGETAAPKPGPEMEKLKFLLGKWNAT
                     VTYEKTPLFPQGGEARGPYTAKLGPGGFSVIADFEGTGGPEGAFQGHEIITWDPQENG
                     YKRYTFGNSFPGAFVSNGHWEGQKLVFEGDLEFGGAKFHFRNELTREAGGAVTIKESY
                     KTGDAPTMLMATTRAIKGGSMGRPVVHFEIGGRDIAKTQEFYAKLFDWKITPMGPAAM
                     IDTGEGIPGHITALGHEPNHYTIFYVDVDDVAAYLEKVKGLGGKTLVPPVEIPTGTFA
                     WFMDPEGNTIGLWKSKK"
     gene            complement(4220..4957)
                     /locus_tag="VGR03_02810"
     CDS             complement(4220..4957)
                     /locus_tag="VGR03_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1993239.1"
                     /translation="MSRNQPREPLPGFTGTARRKPSSLFIVRDLDRAACLIHPIKRRI
                     LAALVQRDSASGLARRLRIPRQLVWYHMQEMARERMVIPAGRRKRRQFVDQCYVARAR
                     TYLLSPELLGKLAADPKTAGDRFSASYLLGLASQVQTEVTQDLELAGEEGKRLATLSL
                     NAELRFTSKEQRAKFTEELERAVVELAAQHSSPYKNADGSAGQGRAFRLVLGCYPIPG
                     TKERGAAGAKDEKAGPSPETLRTREMA"
     gene            5168..>5419
                     /locus_tag="VGR03_02815"
     CDS             5168..>5419
                     /locus_tag="VGR03_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV1993240.1"
                     /translation="MATSVVTKTAAKGGSFLLETPRPEEMFTPADLTGDQKLIGQTAE
                     EFVTKEVLPFAKELEEKKPGLMVEMVKKAGELGLLGGGVP"
BASE COUNT          980 a         1837 c         1436 g         1166 t
ORIGIN      
        1 gagcgacgac gtcatcctcg gctgcgccat gcccgaaggc gagcagggga tgaacgtcgc
       61 gcgcatcgct tcgcttcgcg ccggccttcc tgttgaatgc tccgccatga ccatcaaccg
      121 tttttgctcc tcgggtctcc aggccatcgc cctcgccgcc gagcgcatcc gctccggcgc
      181 cgctgacgtc atcatcgccg gtggcaccga gtccatgagc atggttccca tgggcggcaa
      241 caaagtctct cccaatccct ggctcgtgga caattccccg gacacctaca tcagcatggg
      301 cctcggcacg gaaggcatcg cccgcaaata cggcatcacg cgcgagcagg ccgaccagtt
      361 ctccctcgcc agccaccaga aggctctcgc cgccatcgcc gccggcaagt tcaaggacga
      421 aatcacgccc gtcgaggtga gcatcacttc ctggcccaac ggcaatggcg acggtgcgcc
      481 gaggccgaag accacgccca agccgtccac gcaatccttc gtcttcgaca ccgacgaagg
      541 cccccgcgcc gacacctctc tcgacgccct cgccaagctc aagcccgctt ttcacgtgaa
      601 gggcaccgtc acggcaggga acagctcgca gatgagtgac ggcgccgccg ccgcggtcgt
      661 catgtccgaa gcccgcgcca aatccctcgg cgtgaagccg ctcgcgcgct ttgtctccta
      721 cgcgaccgcc ggatgcctgc ccgaggagtt cggcatcggc ccggtcaacg ccatccccaa
      781 agcgctgaag ctcgccggcc tcaagctaga ggacgtcgcc gtcatcgagc tcaacgaagc
      841 cttcgccgcg cagtcgctcg ccgtcatcca gctcgccggc ctcgatccct cgcgcgtcaa
      901 tcccaacggc ggagccgtcg ccctcggcca tcccctcggc tgcaccggcg ccaagctcac
      961 cgcctccatc cttcgcgagc tcgctcgccg caacgcccgc ttcggtttgg ttaccatgtg
     1021 catcggcggc ggcatgggcg ccgccggcat cctcgagcgc ttgtagctcc tgtagcacag
     1081 gcactctcgc ctgtgctctc ttccgcaacg acctcgagtg tctggaccac acgcttccgt
     1141 ctctgctctc ccgcttggct cccactctgt cgcgccccgc gttctgatgt tatttctgct
     1201 cgatctgccc acccacccgt catcccgagc gcagtcccgg tcttcgaccg ggacgcagtc
     1261 gagggatctc tcttctcatt ctttccgcac tgagaactca aatctgccaa ctcagtagtg
     1321 ggtcagtttg gctttccacg ggcggcggtg taaagtgaag cccccgagcc gctgcggatg
     1381 ccaggtgaca gatgcatagg cgggaatttc gttatagacc ggaattcaag tggcaagtat
     1441 caacagattt gatggcttca cagtgatgga tttcccgctg tccacccgct cacttcccga
     1501 gacaaccgat ggctacgttt acgttttttg ctggattcgc gacggtgtcg aaagtcaatt
     1561 ctatgtaggc cagacgaagc ggcttgcggg gaggatgaac gattaccgtc tggcgcaatt
     1621 tgcagcctgt acggacttcc gtgtcggtga ggctgtgcgg tatttgagag acagcaagaa
     1681 gtgtcggatt gtcgttaggt ataggacgag cgcagcgatg cggaaagatg agtatggcct
     1741 tattagagaa ctacaactat ctggcgttcg gttgatgaat gaattttcga gctacgacta
     1801 ccggacagca aacgagacag aggaaagagc ggcggtgcag aggttttgcg atgtgataat
     1861 aagtaaatcc gaattttgga aagctggtga aatcggggac catgcttcgg agcctcataa
     1921 ggttgagggt ctggtggtca aagggaggag cgtcttgaaa gacagtgagt taacaaacca
     1981 tgatcgcttt gtcgaggcat ttggagggca tgccaaccgg gtatactcaa caggagagat
     2041 tagaacaatc ctgaaagcgc gatatcgaga tttctcggat gggagcgtct tgcccaatga
     2101 ccatgcagac ggtaacaaga gctcatgctg gtgcgctgga aaagactcgc gaatcttcga
     2161 caggctaaag cgcaattcat accgtgttcg accgtcgtcc cgtgcagatc ggacaaggtt
     2221 atagggggtg actttgcaaa ctgacccact tccactgcct cttccgcatt gacacatcca
     2281 ctcttccgct atagcttccc attcgcatct cttcgcggag gtcttcctat gaaacttctc
     2341 tgcttcgccg ctcttttcct tctttccttc gctctcagcg cccaggctcc cgcgccggtt
     2401 cccctcaaga gcgagcccca ccaccatctc atcctcgaaa acgaattcgt ccgcgccttc
     2461 tatgtcgaag tcgccccgca cgacgccacc cttcttcacc agcacgacgc cgactacgtt
     2521 ttcgtcgttc tcgggcccgg cgacttcgtc aacgccgtcg tcggcaaacc tgaagttcac
     2581 gccacactcg acgatgcctc cgttcgcttc tcccgcggcg gcttcgccca catcgcccgc
     2641 accgacgccg gcgtcccctt ccgcaacatc accgtcgagt ttactcgccc ccagggcgcg
     2701 ttgcgcaatc tctgccagaa ggtcaccgac ggcgctgtca acaactgtcc ggattcgccg
     2761 tccccttcga tgaagccgct attcgaaacc gatgagatcg tcgtctcctc cggcgcactc
     2821 gacgccaaat cgaagcacac cgaagcggcg ccgcacactg ctgccctgat cctggccctg
     2881 gagaattcca atctcgacgt caatgtgggt ggcgatgtga aggcgcaact gcgctctggt
     2941 caggtcttct ggctccctgc cggtcggaag cacaccatct cttctcgtgg cggcgcttgc
     3001 cgcttcgtcc tcgtctcctt caaggactcc gccgcgcact agtccctgct tcttccgtgt
     3061 tgaatttttc tcttcgtctg gaggaacagc ggccctttag gagcggcccc ggtggaagag
     3121 cggcccttta gggccgcgta gcccagtcac gagtcactcg tcacatgtca ctttttagac
     3181 ttccacaatc caatcgtgtt cccttcgggg tccatgaacc acgcaaacgt ccccgtcgga
     3241 atctccaccg gcggcaccag cgtctttccg cccagcccct tcactttttc caggtacgcc
     3301 gccacatcgt ccacatccac gtagaaaatc gtgtagtgat tcggctcgtg ccccagcgcc
     3361 gtaatgtggc cgggaatccc ttcgcccgta tcaatcatcg ccgccggccc catgggcgtg
     3421 atcttccagt cgaaaagctt tgcgtagaac tcctgcgtct tcgcgatgtc tcggccccca
     3481 atttcaaaat gcaccacggg tcgtcccatg ctccctccct taatcgccct cgtcgtcgcc
     3541 atcagcatcg tcggcgcgtc gcccgtcttg taactctctt tgatggtcac cgcgcccccc
     3601 gcctcccgcg ttaactcgtt tcgaaaatga aatttcgccc cgccgaactc cagatctccc
     3661 tcgaagacca gcttctgtcc ctcccaatgg ccgttggaaa cgaacgcgcc cggaaaacta
     3721 ttgccgaagg tataccgctt gtacccgttc tcctgggggt cccacgtgat gatttcgtgt
     3781 ccctggaagg ctccttcggg tcctcctgtc ccctcgaaat cggcaatcac cgaaaaacca
     3841 cccggcccga gcttcgccgt gtagggccct cttgcttctc cgccttgcgg aaagagcggc
     3901 gtcttctcgt acgtcaccgt cgcgttccac tttcccagca gaaatttcag cttctccatc
     3961 tccggccccg gcttcggcgc cgctgtctct ccctgcgccg ccggtttgtc gccctgctgt
     4021 tgccgcgccg ggactcccac cgcccacaag ctcatcaaca caacaccgca agccaccatc
     4081 attctgattc tcatcgctcg tcctttcgcc tttctcctat gactcatctc tctctccgtg
     4141 ctctccgtga tcctctgttt cctctccagc cgcaccatgg gtccgcgatg ctcgaccggt
     4201 ttctcgctgc catcgccgct catgccatct ctcttgtcct gagcgtttcc ggcgaagggc
     4261 ccgccttttc atccttcgcc ccggcagctc cgcgttcttt cgtccctgga atcgggtaac
     4321 accccaggac cagccggaac gcgcgcccct gccccgcgct tccgtccgcg tttttgtacg
     4381 gcgagctgtg ctgcgccgcc agctccacca cggcgcgctc aagttcttcg gtgaacttcg
     4441 cccgctgctc cttcgatgtg aaccgcagtt ccgcgttcag cgaaagcgtc gccagccgtt
     4501 ttccctcttc gccggcgagc tcgaggtcct gtgttacttc cgtctgaacc tgcgaggcga
     4561 gtcccagaag ataactggcg gagaaacggt ccccggcggt cttcggatcg gccgccagct
     4621 ttccgagcag ttccggcgac aggaggtaag tgcgcgcccg cgccacgtag cattggtcga
     4681 caaattgtct ccgcttgcgc cgccccgccg ggatgaccat ccgctctcgc gccatctcct
     4741 gcatgtggta ccacaccaac tggcgcggaa tgcgcagccg tcgcgccagc ccggatgcgg
     4801 aatcccgctg caccagcgcg gccaggattc gccgcttgat cggatggatc aggcaggccg
     4861 cccggtccag gtcccggacg ataaagagcg atgagggctt ccgccgggcg gtccccgtga
     4921 agccggggag aggctctctg ggttggtttc ggctcatttg catggcgcgt attttcactt
     4981 gtcaaaaagt tttgtcaagt aaaatctttt tgacaagtca aatgctggct tttttgctgt
     5041 ttttgaccct aaatcgccct ctttttttcc cctttttggc cctccctgaa tccgcccctc
     5101 tcccctcccc gaagcgcgct ataatgagcc gggaaaaaag cggcgctcgc gcgcaaggag
     5161 aatcaccatg gcgacttcgg tcgtgacgaa gacggcggcg aagggtggaa gttttttgct
     5221 cgagacgccg cgcccggagg aaatgttcac tcccgccgat cttaccggcg atcaaaaact
     5281 gatcgggcag acggcggaag agtttgtgac caaggaagtg ctgccgtttg caaaggaact
     5341 cgaagagaag aagcccggcc tgatggtcga gatggtgaag aaggccggtg aactgggttt
     5401 gctcggcggc ggcgtgccg
//
LOCUS       DASYVO010000090         1880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_1660, whole genome shotgun sequence.
ACCESSION   DASYVO010000090 DASYVO010000000
VERSION     DASYVO010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VGR03_02820"
     CDS             <1..853
                     /locus_tag="VGR03_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993241.1"
                     /translation="LFVLAVCSPSPALAQENPYFVAYDHYLEEPGNLEIEYFSTLGTQ
                     RGGNKFHAFWAEFEYGATAWWTTELYLDGQTTFRESTVFTGFRWENRFRLLKREHFIN
                     PVFYVEYENKNGADKILKEGEGHDVESDFLVSNAEARKEHIHEIELKLILSSTFKGWN
                     FTENTLAAKNLANSPWEFGYALGASRPLALKASAKRCTLCPQNFIGGAEMYGGLGDRH
                     SFGLHDTSHYLAPVLAWNLPSGWTLRISPGFGLNDNSHRCLMRWGVSREITGFGSMVS
                     RLLGGRT"
     gene            850..1320
                     /locus_tag="VGR03_02825"
     CDS             850..1320
                     /locus_tag="VGR03_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEV1993242.1"
                     /translation="MRAIAAALAVCTSFLAASIVLAQGRNSDKPPADAAPRYDPAIYA
                     ELQKAPEKARNRANPLQNDADAVAAGAILFEQHCAECHGNSAEGSRKSPSLLVPEVQS
                     ATPGTLFWLLTNGVVRKKMPVWSKLPEPQRWQLVRYIKLLGGSAPAAESPAPKP"
     gene            complement(1354..>1880)
                     /locus_tag="VGR03_02830"
     CDS             complement(1354..>1880)
                     /locus_tag="VGR03_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1993243.1"
                     /translation="HGTLQLFPASRRTQVKETLEDLRDVLRDYLAGMTIVTLVVIAAS
                     SLFFWMIGMEYPILTGIASGLLNMVPYIGAVMAWLPAFIIALAKWRTLGQFVLIAATL
                     TTIHILAINLVAPQLVGRRVRLNAVAITIALLFWGWVWGGMGLVLAIPITAALRVICD
                     HTESWKPIGRWLSA"
BASE COUNT          401 a          586 c          519 g          374 t
ORIGIN      
        1 ccttttcgta ctcgccgttt gctctccatc tcctgccctc gcccaggaga acccttactt
       61 tgtcgcctat gaccactatc tcgaagagcc cggcaatctc gagatcgagt atttctccac
      121 cctgggcacc cagcgaggcg gcaacaagtt ccacgccttc tgggccgagt tcgaatacgg
      181 cgcaaccgcc tggtggacca cggagttgta tctggacggc cagaccacgt ttcgcgagag
      241 caccgtattc actggtttcc gctgggagaa tcgcttccgg ctcttgaagc gcgaacactt
      301 catcaatccc gtcttctatg tcgagtatga aaacaagaac ggcgcggata agattctcaa
      361 agaaggcgag ggccacgatg tggaatcgga cttcctcgtt tcgaacgcgg aagcccgcaa
      421 ggagcatatc catgaaattg agttgaagct catcctgtca agcacattca agggctggaa
      481 cttcacggag aacaccctcg ctgccaagaa tcttgcgaat tcaccatggg aattcggcta
      541 cgcccttggt gccagccgcc cgctcgctct gaaggcttcc gcgaaacgct gcactctctg
      601 tccgcaaaac ttcattggcg gggccgagat gtacggcggc ctcggtgacc ggcatagttt
      661 tgggcttcac gatacatcgc attacctggc gcccgttctc gcctggaatt taccctccgg
      721 ctggacgttg cggatttcgc cgggctttgg cctgaacgac aacagccacc ggtgcctcat
      781 gcgctggggc gtgtcgcggg aaataactgg gttcggttcg atggtcagcc ggctccttgg
      841 aggccgcacg tgagagccat tgccgcggca cttgccgtct gcacttcatt tcttgcggca
      901 tctatagttc tcgcacaggg ccggaattcc gacaagccac ccgccgacgc agcgcccagg
      961 tacgatccgg ccatctacgc ggaattgcaa aaggcgccag aaaaggcgag gaaccgcgca
     1021 aatccgctgc agaacgatgc agacgccgtt gccgccggcg ccattctatt cgaacagcac
     1081 tgcgcggaat gccacggaaa ctccgcggaa ggctcacgca aatctccgag tctcctggtt
     1141 ccagaggtgc aaagcgctac gcccggaacc ctattctggc tgttgaccaa tggagtggtt
     1201 cgaaagaaga tgccggtttg gtcgaagctt ccggaacccc agcgttggca gttggtgcgg
     1261 tacatcaagt tgcttggtgg gtccgctcca gcggcggaat ctcccgctcc gaaaccttga
     1321 cggaagaatc cgttttctgc tcacgccgcg gtttcaggcg ctgagccagc gcccgatcgg
     1381 tttccaggat tcggtgtgat cgcagatcac gcgcagggcc gcagtgatgg gaatggccag
     1441 aaccaatccc atgcctcccc atacccagcc ccagaagagc agcgcgatgg tgatcgccac
     1501 cgcattcaag cgcacgcggc ggcccaccag ttgcggcgct acaagattga tagcgagaat
     1561 gtggatggta gtcagcgtcg ccgcaatcag aacgaactgc ccgagcgttc tccacttcgc
     1621 caacgcaatg ataaaggccg gcagccaggc catcactgcc ccgatgtacg gcaccatgtt
     1681 caacaaaccg gacgcgattc ccgtgaggat cggatactcc attccgatca tccagaaaaa
     1741 caggctgctc gccgcgatta ccaccagcgt cacgatggtc attcccgcta ggtaatcgcg
     1801 cagcacatcg cgcaaatctt ccagcgtttc cttgacttgc gtccgccgcg aggcgggaaa
     1861 aagttgcaac gtcccgtgcc
//
LOCUS       DASYVO010000091         1937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_158557, whole genome shotgun sequence.
ACCESSION   DASYVO010000091 DASYVO010000000
VERSION     DASYVO010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158557"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VGR03_02835"
     CDS             <1..297
                     /locus_tag="VGR03_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993244.1"
                     /translation="FLGHELGLTRADDVSRIVALIGRLGPLPPWPRVPPAALLNAMRS
                     DKKTRAGVLRFVLSPRIGEARSYDTVPLHVVERVLHFTPQLITASNKLSRRRHG"
     gene            290..1069
                     /gene="ubiE"
                     /locus_tag="VGR03_02840"
     CDS             290..1069
                     /gene="ubiE"
                     /locus_tag="VGR03_02840"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEV1993245.1"
                     /translation="MAEAAAPVKGTRPEGAVTEADASKKVREIFTEIAPRYDFLNHLL
                     SLQLDRLWRARAAKHLQPVFLNRETCVLDLCCGTGDLAIVLNRRYRARVIGVDFAYSM
                     LVRARKKSARLHSPDLAQLRPIKYIEADALRLPFADGTFDLVTTAFGFRNLANYEAGL
                     REIQRVLKPGGTVAILEFAEPPQGLLGDLYRWYFCKVLPKIGGLISGDQAAYKYLPKS
                     VARFFRPPELAALLSAVGYHSVDYRVWTFGTIALHTALRAK"
     gene            complement(1128..>1937)
                     /locus_tag="VGR03_02845"
     CDS             complement(1128..>1937)
                     /locus_tag="VGR03_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV1993246.1"
                     /translation="FVALNCAAIPEGLVENELFGHERGAFTGAGARKVGKMDLAHRGT
                     LFLDEIGELPLAIQAKLLRVLEERRFERVGGTQSIDVDVRIVVATNRDLQKLAEEKLF
                     REDLYFRISAVPMTIPPLRERGNDVLLLADHFLEKFSREFGKPGLELSAEARDRLLHY
                     RWRGNVRELQNTLERAVILADGLSIRPEGLQLPASKPDAERLPAGMLPGNFNWEGSLE
                     EVTERAVSHVEKALLENTMRECKWNKTRAAEKLGVSPKTLLAKLRSAGLEE"
BASE COUNT          352 a          668 c          503 g          414 t
ORIGIN      
        1 ttcctcgggc atgaactcgg cttgacgcgc gcggacgatg tttcccgcat cgtcgcactg
       61 attggccgac tgggacctct gccgccctgg cctcgcgttc cgcccgcggc gctgcttaat
      121 gccatgcgct ccgacaagaa aacgcgcgcc ggagttcttc gcttcgtcct ctcgcctcgc
      181 atcggcgaag cgcggtcgta cgacaccgtc cctcttcacg tcgtcgagcg cgttctccat
      241 ttcacgcctc agctcatcac tgcgagtaac aagctttcgc gaaggcgcca tggctgaggc
      301 cgctgcaccg gtcaagggaa cccggcccga gggcgccgtt accgaagccg acgcttcgaa
      361 aaaagtccgc gaaatattca cagagattgc gcctcgctac gattttctaa accatctcct
      421 gtcgctgcaa ctcgatcgcc tttggcgcgc ccgcgctgca aagcatcttc agcctgtctt
      481 tcttaaccgg gagacctgcg tactcgacct ttgctgcgga acaggagacc tcgcaattgt
      541 gcttaaccgg agatatcgag cgcgagtgat cggcgtggac tttgcataca gcatgctagt
      601 gcgcgccaga aagaaaagcg cccgcctgca tagtcctgat ttagcgcagc tccgcccgat
      661 caagtatatc gaagcggatg cgttgcggct cccgttcgca gacggcacct ttgatctcgt
      721 cacgaccgcc ttcggctttc gcaatctcgc caactacgaa gccggcttgc gagagattca
      781 gcgcgtgttg aagcccggcg gcaccgtcgc gattctggaa ttcgccgagc ccccgcaagg
      841 cctcttaggg gacctctatc gttggtattt ttgtaaggtt ctgcccaaga ttggcggtct
      901 catctccggt gatcaggcgg cctacaagta tctcccgaaa tccgttgcgc gattcttccg
      961 cccgcccgaa ctcgccgcgc ttctgagcgc cgtcggctac cattctgtgg actatcgcgt
     1021 ctggaccttc ggcaccatcg ccctgcacac cgccctgcgc gcaaaataat tagtagagcc
     1081 gggcggaacc tgtcccgacc cggtcaggag cccggccttc ttgcccctta ctcttccagt
     1141 cccgcacttc gcaacttcgc cagcagcgtt ttcggcgaga cgcccagctt ttccgccgcc
     1201 cgcgtcttat tccacttgca ctcgcgcatc gtgttttcca gcaaagcttt ttccacatgg
     1261 ctcacggcgc gctcggtgac ttcctccagc gagccttccc agttgaaatt ccccggaagc
     1321 atgcccgcag gcagtcgctc ggcatccggc ttcgaagcag gtaactgcaa gccctccggg
     1381 cgaatgctca acccatccgc caggatcacc gcccgctcca gcgtattctg caattcgcga
     1441 acgtttcccc gccagcggta gtgcagcagt cggtctcgcg cctcggctga caattccagt
     1501 ccgggctttc caaattcgcg gctgaatttt tccaggaagt ggtccgccag gagcaggacg
     1561 tcgttcccgc gctcgcgcaa gggaggaatc gtcatcggaa ccgccgaaat tcgaaagtac
     1621 aggtcttcgc ggaacagttt ttcttccgcc agtttctgca gatcgcgatt cgtggccacc
     1681 acgatgcgaa cgtccacgtc gatcgattgc gtcccgccca ctctctcaaa tcgccgctct
     1741 tccagcacgc gcaacaactt tgcctggatg gccagcggca actcgccaat ttcatccaga
     1801 aaaagcgttc cgcgatgcgc caggtccatc ttcccgacct tgcgcgcacc cgctccggtg
     1861 aacgcacctc gttcgtgccc gaagagttcg ttctccacca gcccttccgg gatggccgcg
     1921 caattcagcg ccacaaa
//
LOCUS       DASYVO010000092        20268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_30282, whole genome shotgun sequence.
ACCESSION   DASYVO010000092 DASYVO010000000
VERSION     DASYVO010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 20268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20268
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30282"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VGR03_02850"
     CDS             <1..324
                     /locus_tag="VGR03_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993247.1"
                     /translation="FPNRDRRLWPGQFVNTIVRLAEQTNAIVVPAQAIETGQKGTFVY
                     VLKADQTAEIRPVTVERNAGGYAVVSSGLRPGETVVTDGQIMIFPGARLQVKSSPASS
                     VPPGQ"
     gene            335..3562
                     /locus_tag="VGR03_02855"
     CDS             335..3562
                     /locus_tag="VGR03_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV1993248.1"
                     /translation="MNVAEPFIRRPVMTTLFMAAILLFGIIAYRALPVSDLPNVDRPT
                     IQVFATLPGASPDTMASAVATPLEKQFSTIAGIDAMTSTSTQGFSTVSLQFDLSRNID
                     AAAQDVQAAISRAAPLLPQNMPSPPTFQKTNPADQPILYMSVSSPTMPLSQVDEYAET
                     NLSERISMIKGVAQVTVYGSQKYAVRIQVDPRALASRKIGLDEVSAAVQQGNVNLPTG
                     TLSGAHKAFTVQANGQLLRADGYRPLIVAYRNGLPVRLGDLGVVADSVEGNRVASWYN
                     NEPSIVLAIQRQPGTNTIEVVDAIKRVLPVIRQQIPPSVSLTVLYDRSESIRASVADV
                     KFSLMLAVCLVVLVIFLFLRNLSATLIPSIALPMSIIGTFAVMYLLDYTVDNLSLMAL
                     TLSVGFVVDDAIVMLENIVRHMEHGEGVMEAALKGSREIGFTIVSMTLSLAAVFIPVL
                     FMGGILGRLLHEFSVVIMVAILVSGVVSLSLTPMLCSRFLRPPGQLRHGRAYMKLEGF
                     FDGMLRLYDRTLGWVLRHRGATMLVSAAILIATLWQFVVMPKGFLPSEDSGQIFAITL
                     AQQGISFDAMKEHQQALNEVTLRDPNVDKFFSFCGASGFLGAGNTGILFLRLKAHDER
                     QWIGNESFHRLEQRWGRKPVLGALLAIIGPFFGHHLTVEELIEELRPKYAAVPGILAF
                     MQNPPPIEIGGHLTKAEYQFTLQSPNTQELYPASNFLLAKLRELRGLQDVNSDLQISN
                     PQVNVEVNRDRAATLGLSAQQLEDALFTAYGQRQISTIYTPNNEYRVILELEDSFQRD
                     PASLNLLYVRNATGQLVPLSSAADLTTGLGPLVVNHLGQLPAVTISFNLKPGTSLSEA
                     LDSVQKLARETLPSTVSTSFQGSAQAFSQSEGGLAVLLIVAVLVIYIVLGILYESFLH
                     PLTILSGLPSAGFGALLTLWLFHYELNLYGFVGIIMLIGIVKKNAIMVVDFALEAQRS
                     GGKSAAEAAHQGSLVRFRPIMMTTMAALMGTLPIALGVGAGAGSRRPLGLAVVGGLLF
                     SQLVTLYLTPVFYTYVDAFQSRFAGKSRKSVTRPAAVPGTSVSAD"
     gene            3765..5300
                     /locus_tag="VGR03_02860"
     CDS             3765..5300
                     /locus_tag="VGR03_02860"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HEV1993249.1"
                     /translation="MSNKLASATDPVRVEQGQLSGTAGTHPDVRVYRGIPFATPPVGE
                     LRWKAPHPPASWQGVRQATKFAPACWQTPYPAAAMIFQTELPTLSEDCLYLNVWTTAK
                     SAKERLPVMVWIHGGGFTRGLGSSSAYDGENLARKGVVVVTINYRLGIFGFFAHPDLT
                     AESERHASGNYALLDILAALHWVKQNIASFGGDPDRVTVFGESAGSWAINALMASPRA
                     KGLFHRAIGESGGCFFPLKTLAEAEKAGLHLASTVGAAQDALKTLRAKSAEELLRAGS
                     EDTVRPLMDGWVLPQQVYTIFAEGKQNDVPLLVGSNADEGTALVPQGINLKAALFVVG
                     VRQRYGNLAHQFLKVYPAGSDEEAVASFYAAYRDQIFGWEMRTWARMEAKSGHHPAYL
                     YYFSRRPPGPQSRRLGAFHAADIAYVFGNFTWPFPWEEADRKLSDAISSYWVNFAATG
                     DPNGAGLATWPAYNAATDQSLEFGDRITVRSEINKPGLDFFDAYYASLAGTQEPAAAG
                     SKK"
     gene            5327..6577
                     /locus_tag="VGR03_02865"
     CDS             5327..6577
                     /locus_tag="VGR03_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV1993250.1"
                     /translation="MSNRQVNTMNFEFSDKTKDLQRRLQTFMDEHVYPNELRFQNEIE
                     RERWTPTRIIEELKPKARAAGLWNLFLPESEYGAGLTNLEYAPICEIMGRSVLAPEVF
                     NCSAPDTGNMEVLARYGTPEQKERWLKPLLAGEIRSCFAMTEPGVASSDATNISASIV
                     REGNEYIINGRKWWASGAGDPRCKIAIFMGKTDSAAPAHKQQSMVLVPMDAQGVKIVR
                     LLTVYGYDHAPHGHAEITFENVRVPVSNMLLGEGRGFEIAQGRLGPGRIHHCMRCIGV
                     AERALETMCKRVQSRVAFGKTLAEQGTIRADIAASRMEIEQARLLTLKAASMMDTAGN
                     KAARAEIAMIKVVAPNVALRVLDRAIQAYGGAGVSQDTFLAGAWANVRTLRLADGPDE
                     VHTESIARGELRKWGPPPPRSTPR"
     gene            6636..7100
                     /locus_tag="VGR03_02870"
     CDS             6636..7100
                     /locus_tag="VGR03_02870"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEV1993251.1"
                     /translation="MPPLIIENLPSLKEFVGKEIALTEWFRVTQDRIQQFAEATEDRQ
                     WIHLDPERARNESPYGTTIAHGFLTLSLISHLLKQAIEIRAGVRLAINYGLNRVRFPS
                     PVRAGANVRARFVLLALKEQGNALEAVYSVSIESEGAEKPSCVAEWIVRYYP"
     gene            complement(7149..8216)
                     /locus_tag="VGR03_02875"
     CDS             complement(7149..8216)
                     /locus_tag="VGR03_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV1993252.1"
                     /translation="MADYRDQSTAVRAGEELDLAKLEPYLRNLFPGESGPLLVEQFPS
                     GHSNLTYSVRLGGKQFVLRRPPFGSKVKSAHDMGREFRVLSKLHAVYPAAPKVLAHCE
                     DETVLGAPFYLMEPIRGIILRKDPAPGFAFPAETARRLSENFVDNLARLHGLDYEAIG
                     LGDLGKPQGYVERQVKGWTERYHGSQTHDLPEVERISAWMREHMPSAGGAALIHNDYK
                     YDNVVLDPNDITKIAGVLDWEMCTIGDPLSDLGTTLAYWIDAQDPEELQRIRWGPTMV
                     PGSLTRAQIVERYAQTAGRDISGMVFYLAFARFKVAVIVQQIYYRYHHGLTKDERFAA
                     MPELIKLLLRASLRGAENESL"
     gene            complement(8341..10464)
                     /locus_tag="VGR03_02880"
     CDS             complement(8341..10464)
                     /locus_tag="VGR03_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEV1993253.1"
                     /translation="MSDLIQLSTENGIAVITINNPPVNALSPGVPEGIAAAIEQIDGD
                     ETVRAAVLIGGGRTFVAGADIKEFGKMTAGKSRGPGLLPLLLRIEDCRKPMVVAIHGT
                     AFGGGLELAMAGHYRVAAPGAQVGQPEVKLGIIPGAGGTQRLPRLAGVAKAVEMCAEG
                     NPIKAEEAVKFEIVDRIIEGDLLAGAVAFAREIAGKPAPKTRERNQKLGMPEQNAPIF
                     AAARETARKKQRGTIAPLAAIDAIEAGTKLAFEAGCEAERKLFMQCLLSDQSKALIHV
                     FFGEREVAKIPDVPKETPVLPVNSAAVVGAGTMGAGIAMVFANAGIPVLLKEADQAAL
                     DRGLSNIQKNYSNSVKRGRFTEAFAAERLKLIRPTLTYEGFGGADMVVEAVFEGMALK
                     KEVFGQLDRVCKTGAILASNTSTLNIDEIASATSRPGAVIGTHFFSPANVMRLLEIVR
                     GKATGKEVIATCMQLSKRLGKVGVLVGNCTGFVGNRMFGPYRREAQFLIEEGAGVEAV
                     DSALYEYGMAMGPLATGDLAGLDVGWRIRKEYRHLQKPGVRQPIAEDRLCEMGRYGQK
                     TGAGWYKYDEQRRAIPDPEVEQLVRKWAGEAGIPQRKIAAEEIVDRCVYALVNEGARI
                     LEEGYALRAVDIDVIYLYGYAFPSYRGGPMWYADTVGLRKVYERICEFRREHGELWEP
                     APLLKQLAEQGKTFAEFNKEQGAAA"
     gene            complement(10491..11594)
                     /locus_tag="VGR03_02885"
     CDS             complement(10491..11594)
                     /locus_tag="VGR03_02885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV1993254.1"
                     /translation="MQFGLSESQEILKDSARKFFTGECPMAEVRRVMETETAYDAALW
                     AKLAAQGYTGVIFPEEYGGAGLGKVELILLMEEAGRALLPGPFFSTVALGGAVLDSAG
                     SAEQKKKYLAPICQGEARSTVAILESSASWDPADAEITVVNGKLTGEKLFVSDAAVAD
                     FLIVVAHNGVFIVDGRAHGIRTTAMPAMDLTRKLYSVEFQDTPAERLGHEAGLARGLQ
                     IATAALVAEMVGGMQRTLDLTVEYAKTRKQFGKPIGTFQAVQHPCADMYLETESSRSA
                     AYYAGWALEENVADAAAAVSIAKMYASDASRTVGNRGIQIHGGMGFTWENDLHLFYRR
                     AKSSETALGDATFHRERLARMVIDAGAAAARSA"
     gene            complement(11710..12897)
                     /locus_tag="VGR03_02890"
     CDS             complement(11710..12897)
                     /locus_tag="VGR03_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEV1993255.1"
                     /translation="MDLNLTAEELEFRDGLHAWLAANAPKDWDKQREKSLDHRFEFMR
                     AWQRKLYEAGWAGISWPKEYGGRGANLMEQVLFWQEMARAGAPPMANVLGLGLIGPTI
                     IAFGSEAQKKRFLRKILSAEEIWCQGFSEPNAGSDLANLQTEARLDGDHYIVNGQKVW
                     TSYGWVGDWCEVVVRTDPNVPKHKGLTVLLLDMKTPGVDVRPLRQMTGETEFSELFFR
                     DVRVPAENVLGGVNRGWEVAMGTLMHERGSFGAGLQIIYKRNMDRLIELSRTVRRNGR
                     PAAEDPLLRQKLAQCYAEIEIMRLNQMRAFSRISATGVPGPEGSILKIFWSELNQRLQ
                     QIAQELLGPYGQLEGSGDHAVDNGMWSYGYLRARGNTIEAGTSEIQRNIIGHFVLGLP
                     RSY"
     gene            complement(12925..13692)
                     /locus_tag="VGR03_02895"
     CDS             complement(12925..13692)
                     /locus_tag="VGR03_02895"
                     /inference="COORDINATES: protein motif:HMM:NF006070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1993256.1"
                     /translation="MNVKQLFDLTGRVAIISGGSMGLGRQMAEGLAEMGANLVLCARK
                     KERCQQAAEELQKLGVRTLALGCDVKNPAQIQEVVDATVSEFGRIDILINNAGTSWGA
                     PVEEMRLEHWNKVIETNLTGTFLFSQAAGKIMIRQGRGKIINIASVAGLRGSPPEFQA
                     IGYHASKGGVIALTKDLACKWGMHNIQVNAIAPGWFPTHMSEVLIERNRDLFLKKVPL
                     GRFGNDYDLKGAAVFLASDASGYVTGHVLIVDGGQSA"
     gene            14134..14853
                     /locus_tag="VGR03_02900"
     CDS             14134..14853
                     /locus_tag="VGR03_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993257.1"
                     /translation="MKKYRSLHWTVMLAFAVPVGGLVFGGLALAQRTLEKTLIVNGKP
                     VAGAMADMQGRTYVDLQMLSQTLGGTLTFETDRITLVIPAAGAGNSQQTRPQAPTSQP
                     PTPEHLSTNFRTAAITALGEMRQWQGAVESVITYGIPVAGSWPQDYRDRAESGINQAK
                     VVASTDEDRSALLLLENDFASLRNWSDTVVTERKSLHGARFVDPDALKKDTALAKITD
                     CSRFLGGMISGGVYSDNASCH"
     gene            complement(15120..15812)
                     /locus_tag="VGR03_02905"
     CDS             complement(15120..15812)
                     /locus_tag="VGR03_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV1993258.1"
                     /translation="MEGAMSSSNKKSNGISEKKGAFNAQAFLDSAGVARKVKEFKRAE
                     VIYTQGDPAKNVMYLQEGGVKLSVVSEIGKEAVVAILGPGDFFGEGCLAGQSVRMGTV
                     TAITPTTVLVIEKSEMFKVLHEQHALSDRFITFMLARNIRIEEDLVDQLFNSSEKRLA
                     RALLLLARYGKDDKPHGTLPKVSQETLAEMVGTTRSRVNFFMNKFRKLGFIKYNGSLQ
                     INTSLLSVVLHD"
     gene            complement(15886..16113)
                     /locus_tag="VGR03_02910"
     CDS             complement(15886..16113)
                     /locus_tag="VGR03_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993259.1"
                     /translation="MVDEEWIKTLADGRRVKFTNQEMLEAQAFITAQVERNKVVYSIV
                     LTKVKTPLSHEEVEGYFEGELSKKLRHATLN"
     gene            complement(16317..17000)
                     /locus_tag="VGR03_02915"
     CDS             complement(16317..17000)
                     /locus_tag="VGR03_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1993260.1"
                     /translation="MKGVMVTVALADDHVVVRQGLRLLLESDPEFVVVGEAADGIEAL
                     ELVKSRKPKVLIADLMMPGLDGLEITRRVSRLKLDTRVVILTMYGDEVYLLDALGSGA
                     AGFVVKESCGADLFQAIRDVVAGRRYLSPLLSEASTRRYLQKFQTALLKLSDTLTTRE
                     RKVLQLVLEGASSCDIGARLKITLRTVESDLARFVEKLGLNPRQDSIRHTVKRRVRPR
                     GRTKFIKKK"
     gene            17432..18889
                     /gene="kaiC"
                     /locus_tag="VGR03_02920"
     CDS             17432..18889
                     /gene="kaiC"
                     /locus_tag="VGR03_02920"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HEV1993261.1"
                     /translation="MATAKRRAKGRETGGPQRKRKELPKVSTGIAGLDLVLGGGLPAG
                     RVTLLSGGPGSGKTIMGLQYLLHGAGADQPGILLMFEERAAAVRQNAWSRGWDMVRLE
                     KKNKLYLMDARLDPEAVISGEFSISGLLAILDQRIKAMRAKLILIDAVDALLHLYDNP
                     MRERRELYALHEWLLDRGLTTIMTVKTVPQEEVPARYAFLDFMADCVIHVDQRVTAQI
                     TTRRLRVIKYRGSGYGRNEYPFIIDENGINIIPITSNVLQHRPPGPKVSSGQAWLDEV
                     LVGGYKCGTSILIAGTAGAGKTTLACVFTRAACLRGERVLYLNFEESAESMVSNMLSP
                     GLALQPLIKTRTLVVRSYLPEAMGVEEHLFHALKNLDEVQPQHVVVDAISACKRMGSD
                     QAAFEYLMRILNACKERGTTCIYINQTSGLDIVEEISGIGISSIIDTIVLLRHLPIGG
                     AIARQLIVMKSRGSKHSEQFHEFRITDRGIDLVKA"
     gene            18950..19279
                     /locus_tag="VGR03_02925"
     CDS             18950..19279
                     /locus_tag="VGR03_02925"
                     /inference="COORDINATES: protein motif:HMM:NF019309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0048511 - rhythmic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEV1993262.1"
                     /translation="MMKARKRERRRHAKPGGETYVFRLFVAGNESNSSQARGNLARLC
                     EEHIKGRYKIDAVDVLKDAAAAHKNNVLVTPMLILVKPLPKVTVLGNLNDTKQVLAAL
                     RLTGDGR"
     gene            19318..20112
                     /locus_tag="VGR03_02930"
     CDS             19318..20112
                     /locus_tag="VGR03_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1993263.1"
                     /translation="MKEAEELIHALRTQQVDAIVGKRHIMLVRLKQAEENLKSSRNQL
                     RALAAYLLSVRENERAVIARELHDEFGQALTSLHLGLAWISRKMTPGQQPLQEKIRSL
                     SDTTTSLIRSVKNIASELRPGALDELGLVKTLKSEAREFEGHTGIQCRFKTNTAKAKF
                     DRSAAVAIFRIVQAALTNVARHAHASRALVTLMKKKNGLILAVTDNGKGITRKLIYSH
                     DSLGITGMRERALALGGTFALGGSRSKGTILTVQIPWSRVTIGTAP"
BASE COUNT         4002 a         6223 c         5894 g         4149 t
ORIGIN      
        1 ttcccgaatc gtgaccgccg gctttggccc ggccagttcg tgaacacaat tgtgcgactg
       61 gcggaacaga caaacgccat tgttgtgccc gcgcaagcca tcgagacggg ccagaagggc
      121 acgttcgtct acgtgctgaa ggccgatcag acagcggaga ttcgccccgt gacggtggag
      181 cggaatgcgg gcggctatgc ggtcgtctcg agcgggctgc ggcccggcga gacggtggtc
      241 accgacggcc agatcatgat tttccccggc gcgcgtctgc aggtgaaaag ttcgccggcc
      301 agctctgtgc cgccgggaca atgagggctt gcggatgaac gtagcggaac cctttattcg
      361 ccggcccgtg atgacgacgc tgttcatggc cgccatcctg ctcttcggca tcatcgccta
      421 ccgcgcgctt ccggtgagcg acctgccgaa cgtggaccgc cccacgattc aggtgttcgc
      481 cactctcccc ggagcgagcc ccgacaccat ggcgtcggcg gtcgccacgc cgctcgaaaa
      541 acagttttcc accatcgcgg gcattgacgc gatgacctcc accagcacgc aaggcttctc
      601 cacggtctcg ctgcaattcg acttgagccg caacatcgac gcggcggcgc aggatgtgca
      661 agccgcgatc tcccgcgccg ccccgctgct tccgcagaac atgcccagcc cgccgacgtt
      721 ccagaagacg aatccggcgg accaaccgat cctctacatg tcggttagtt cgccgaccat
      781 gccgctttct caggtggacg aatacgcgga gacgaacctg tccgagcgca tctccatgat
      841 caagggcgtg gcgcaggtga ccgtctacgg ctcgcagaaa tacgccgtgc gcatccaggt
      901 ggacccgcgc gcgctcgctt cgcggaagat cggccttgac gaagtttcgg ccgcggtgca
      961 gcagggcaac gtcaacttgc ccacgggaac tttgtccggc gcccacaagg ctttcacggt
     1021 gcaggcgaac ggccagcttc tgcgcgcgga cggctatcgc ccgctcatcg tcgcctaccg
     1081 caacggcttg ccggtgcgtc tgggagatct cggcgtggtg gccgacagcg tcgagggcaa
     1141 tcgcgtggcc agttggtaca acaacgagcc ttccatcgtc ctggccattc agcgccagcc
     1201 gggtacgaac accatcgagg tcgttgacgc catcaagcgc gtgcttcctg tcatccggca
     1261 gcagattccg ccgtcggtga gcctgacggt tctctatgac cgctcggaat ccatccgggc
     1321 ttccgtcgcg gacgtgaaat tcagcctgat gctcgcggtc tgcctcgtgg ttctggtgat
     1381 tttccttttt ctgcgaaacc tttcggcgac gctgataccc agcatcgcgc tgccgatgtc
     1441 catcatcggc actttcgcgg tgatgtacct gctggattac acggtggaca acctgtcgct
     1501 gatggcgctc accctgtcgg tgggcttcgt cgttgacgat gccatcgtca tgctcgaaaa
     1561 catcgtgcgc cacatggagc acggcgaagg ggtgatggaa gccgcgctga agggttcaag
     1621 ggaaatcggc ttcacgatcg tctccatgac gctgtcgctc gccgccgtgt ttattccggt
     1681 gctcttcatg ggaggaatcc tcggccggct gctgcatgaa ttctccgtcg tgatcatggt
     1741 ggcgattctc gtttccggcg tggtctcgct cagcctgaca ccgatgcttt gcagtcggtt
     1801 tctccgtccg ccgggccagc tgcgtcacgg ccgcgcttac atgaagctgg aaggtttctt
     1861 tgacggcatg ctgcggcttt acgaccgcac gctcggatgg gtgctgaggc accgcggggc
     1921 gacgatgctg gtttccgccg ccattctcat cgcgacctta tggcagttcg tcgtcatgcc
     1981 caagggcttc ctgcccagcg aagactccgg ccagattttc gccatcaccc tggcgcagca
     2041 ggggatctcc tttgacgcca tgaaagagca tcaacaagcg ctcaatgaag tcaccctgcg
     2101 cgatccgaat gtggacaagt ttttctcctt ctgcggcgcg agcggcttcc tgggagccgg
     2161 caacacgggc atcctgttcc ttcgcctgaa ggctcacgac gaacggcagt ggatcggcaa
     2221 cgagagcttt caccgtctgg agcagagatg gggccgcaag cccgtcctgg gcgcgctgct
     2281 cgccatcatc gggccgttct tcgggcacca cctgacggtg gaagaactca tcgaggaact
     2341 ccggccgaag tatgccgcag tgcccggcat tcttgctttc atgcagaatc ctccgcctat
     2401 tgaaatcggc ggccacctga ccaaagcgga ataccagttc accctgcaaa gcccgaacac
     2461 gcaggaactc tatccggcgt cgaatttcct cctggcaaag ctgcgcgagc ttcgcggctt
     2521 gcaggacgtg aacagcgatc tgcagatttc caacccgcag gtgaacgtgg aggtgaaccg
     2581 cgaccgcgcc gcgaccttgg gactttcggc gcagcaattg gaagacgcgc ttttcacggc
     2641 gtacggccag cgccagattt ccacgattta cacgccgaat aacgaatacc gcgtcattct
     2701 cgagctcgaa gacagtttcc agcgcgaccc ggcatccctc aacctgcttt acgtccgcaa
     2761 tgcaaccggc caattggtgc cgctgagctc cgcggcggac ttgacgacgg ggctcggccc
     2821 gctcgtcgtc aatcacctcg ggcagcttcc cgccgtgacc atttcgttca atctgaagcc
     2881 cggcacttcc ctgagcgagg cgctcgactc cgtgcagaag ctggcgcgcg aaaccttgcc
     2941 gagcacggtg agcacgagct ttcagggatc ggcgcaggcg ttctcgcaat cggagggcgg
     3001 actggcggtg ctgctcattg tcgccgttct ggtcatttac atcgtcctcg gcattctcta
     3061 cgaaagcttc ctccatccgc tgacgattct ttcgggtctg ccgtccgcgg gtttcggggc
     3121 gctattgacg ctgtggctgt tccactacga actgaacctc tacgggttcg tcggtatcat
     3181 catgctcatc ggcatcgtga agaaaaacgc catcatggtg gtggacttcg ccctggaagc
     3241 gcaacgctcg ggcggcaaga gcgcggccga agcggcgcat caaggatcgc tggtgcggtt
     3301 tcgtcccatc atgatgacca cgatggccgc gctgatgggg accctgccga tcgcgctcgg
     3361 ggtcggtgcg ggcgccggtt cgcgccggcc gctcggactg gccgtggtcg gagggctgct
     3421 cttctcccag ctcgtcacgc tctatctgac gccggttttc tatacttacg tggatgcctt
     3481 ccaatcgaga tttgccggaa aatccaggaa atccgttacc aggcccgccg ccgtaccagg
     3541 aacttccgta tctgcggatt agaatattgt ccgcgtatcc cgctgctgga tggcgcgcgg
     3601 gcagggagct gctccggccg tttcgggaat cagtgcgaac cgtcgcattt ccgtgaaata
     3661 atcttcgcgg tctaatctcc ggggcgtccg gaagggagcc tggtaatcgt atgcgatatc
     3721 tgttatggaa agcgtgctca gtgataatgc tttctcaggc ttttgtgtcg aacaaactcg
     3781 catccgcaac cgatccagtc cgcgtcgagc aggggcagct ctccggcacc gccgggacgc
     3841 atcctgacgt gcgcgtttat cgcggcatcc cctttgccac gccgcctgtg ggagaactgc
     3901 gatggaaagc gccgcacccc ccggcctcgt ggcaaggcgt tcgccaggca acgaagttcg
     3961 ctcctgcctg ctggcagacg ccctatccgg cggcggccat gatctttcaa accgagctcc
     4021 ccacgctcag tgaagactgt ctctacctga acgtttggac cacggccaaa tccgcaaaag
     4081 agcgcctgcc ggtcatggtg tggatccatg gcggaggatt cacgcgcggc ttgggcagtt
     4141 cttccgccta cgacggggag aatctggcgc gtaagggcgt ggtcgtggtg accatcaact
     4201 accggctcgg cattttcgga ttttttgcgc atcccgatct gacggcggag tcggagcgcc
     4261 acgcctccgg aaactacgcc ctgctcgaca tccttgccgc gctgcattgg gtgaaacaga
     4321 acatcgcgag ctttggaggc gacccggacc gcgtcactgt tttcggagaa tcggctggtt
     4381 cgtgggctat caatgcgttg atggcgagtc cacgggccaa aggactgttt caccgggcca
     4441 tcggcgaaag tggcggctgt ttcttccctt tgaagactct ggcggaggcg gagaaagcgg
     4501 gtctgcattt ggcctccact gtgggcgcgg cgcaggatgc tttgaaaaca ctgcgcgcca
     4561 aatcggcgga agaactccta cgcgcgggca gcgaagacac cgttcggccg ctcatggacg
     4621 gctgggtcct gccccagcag gtctacacga tttttgcgga gggcaaacaa aacgatgtgc
     4681 ccttgctggt cggcagcaat gccgatgagg gcaccgcgct tgttcctcaa ggcataaatc
     4741 tcaaggcggc cctgttcgtt gtgggagtgc gccagcgtta cggaaacctg gcgcaccagt
     4801 tcctcaaggt atatccggct ggttcggatg aagaagccgt tgcgtctttc tacgccgcct
     4861 accgcgatca gatcttcggc tgggagatgc gcacctgggc gcgcatggag gcgaaatccg
     4921 gccatcaccc ggcctacctc tattatttca gccggaggcc gcccgggccc cagagccggc
     4981 gcctcggcgc attccatgcg gctgacattg cctatgtctt cggaaatttc acttggccgt
     5041 tcccctggga agaagcggac aggaagctga gcgacgcgat ttccagctac tgggtgaact
     5101 tcgccgccac cggcgacccg aacggcgcgg gactggcaac atggccggcg tacaatgcag
     5161 cgacggacca gtcgctcgaa ttcggtgatc gaatcacggt ccgctcggaa ataaacaagc
     5221 cggggctgga tttctttgac gcctactacg cctcgctggc tggtacgcaa gaacccgccg
     5281 cggccggcag caagaagtag cggacgaatt cgtaatcccg gagcgcttga gcaacagaca
     5341 ggtaaacacg atgaacttcg aattcagcga caagacgaaa gatctgcagc gccgcctcca
     5401 aactttcatg gacgagcacg tctatcccaa cgagctgcgg tttcagaacg agattgagcg
     5461 ggagcggtgg acgccgacac gcatcatcga agagctcaag ccgaaagccc gcgccgcggg
     5521 tctgtggaac ctctttttgc cggagagcga gtacggcgcc gggctgacga acctcgaata
     5581 tgcgccgatt tgcgaaatca tgggccggag cgtcctggcc ccggaggtat tcaactgttc
     5641 ggcgccggat accggcaaca tggaagtgct cgcgcgctac ggcacgccgg aacagaaaga
     5701 gcgctggctg aagcccctgc tcgcgggaga aatccggtcc tgcttcgcca tgaccgagcc
     5761 gggcgtcgcc tcctccgacg ccaccaacat cagcgccagc atcgttcgcg aaggcaacga
     5821 atacatcatc aacggacgca aatggtgggc ttcgggcgcc ggcgatccgc gctgcaagat
     5881 tgcgatcttc atgggcaaaa cggattccgc cgccccggct cacaaacagc agtccatggt
     5941 gcttgtgccg atggacgcgc agggcgtcaa gatcgtgcgc ttgctgaccg tgtacggcta
     6001 cgatcacgcg ccgcacggac atgccgagat cacattcgaa aacgtgcgcg tgcccgtttc
     6061 gaacatgctc ctcggtgaag ggcgcggctt cgaaattgcc cagggccggc tggggccggg
     6121 gcgcatccac cactgcatgc gctgcatcgg cgtggcggaa cgcgcgctgg aaacgatgtg
     6181 caaacgcgtg cagtcccgcg tggccttcgg gaaaactctg gcggaacagg gaacgatccg
     6241 cgccgatatc gcagcatcac gaatggaaat tgaacaggct cgcctgctga cgctgaaagc
     6301 cgcatctatg atggacaccg ccggaaacaa ggcggctcgc gcggagattg cgatgatcaa
     6361 ggtcgtcgcg ccgaacgtgg ccctgcgggt gctcgatcgc gccatccagg cgtatggcgg
     6421 agccggcgtt tcgcaggaca cttttctcgc cggcgcgtgg gccaacgtgc gcaccttgcg
     6481 cctggcggac ggccccgatg aagtgcacac ggaatccatc gcgaggggag agcttcgaaa
     6541 atggggcccg ccgccgccgc gctcgacgcc gcgctgaacg ctatcccctt tcgcctttgg
     6601 gcgcgccgga ttccgcttgc tcggaagagg tcttcatgcc gccactcatc atcgaaaacc
     6661 tgccgtcgct gaaagagttc gtgggcaaag aaatcgcgct gaccgaatgg ttccgcgtca
     6721 cccaggaccg gattcaacaa tttgccgagg ccacggagga ccgccagtgg attcacctgg
     6781 acccggagcg cgcccgcaac gaatcgccct acggcacaac gatcgcccat ggattcctca
     6841 ccctctccct gatcagccat ctcctgaaac aggccatcga gatccgcgcg ggcgtccgcc
     6901 tggccatcaa ctacgggctg aaccgcgtgc gtttcccctc ccccgtgcgc gccggcgcaa
     6961 acgtccgcgc tcgctttgtc ctcctggcgc tcaaggaaca aggcaacgcc ctcgaagccg
     7021 tctattctgt atccattgag agcgagggcg cggagaagcc gtcctgcgtg gccgaatgga
     7081 tcgtccgcta ctacccttga cctttttttt cgagttcttc cgcgaagccg gcgcagacgc
     7141 gaactgtttt agagcgattc gttctcggcg ccccgcaaag aagcgcgcaa cagaagcttg
     7201 atcagttccg gcatggcggc aaaccgctca tctttcgtga gtccgtgatg atagcggtag
     7261 tagatctgct gcacgatgac cgcgaccttg aagcgcgcaa aagccaggta aaaaaccatg
     7321 ccggaaatat cgcgccccgc cgtttgcgcg tagcgctcga cgatctgcgc gcgcgtcagg
     7381 ctgccgggaa ccatcgtcgg tccccagcgg attctttgca gctcctcggg gtcctgcgcg
     7441 tcaatccaat aggccaacgt ggtgcccaga tcgctcaggg gatcgccgat cgtgcacatc
     7501 tcccagtcca ggacccctgc gatctttgtg atgtcgttcg ggtccagaac gacgttgtca
     7561 tacttgtagt cgttgtggat cagcgcggct cccccggccg aaggcatgtg ctctctcatc
     7621 caggctgata tgcgctcgac ttccggcaga tcgtgcgttt gtgagccgtg gtagcgctcg
     7681 gtccagccct tcacctggcg ttcaacgtag ccctgcggct tgcccaggtc gccaagaccg
     7741 atggcctcat aatccagccc atgcaggcga gccagattgt ccacgaagtt ttcgctcagg
     7801 cgccgcgccg tctccgccgg gaatgcgaac cccggcgccg gatcttttcg aaggatgatt
     7861 ccccggatgg gctccatcag gtaaaacggc gcgcccaaaa ctgtttcatc ctcgcagtgc
     7921 gcgagcactt tcggcgccgc tggatagacc gcgtgcagct tcgagagcac gcggaactct
     7981 cgccccatgt cgtgcgcgga tttgactttg ctgccgaacg gcgggcgtcg cagaacgaat
     8041 tgcttaccgc cgagacgcac cgaataggtc aggttggagt ggccgctggg aaactgttcg
     8101 acaaggagcg gcccgctctc tccgggaaaa aggttccgca gatatggctc cagcttggcg
     8161 aggtcgagct cctctccggc tcggacggct gttgactggt cacgatagtc ggccattgtc
     8221 tatctcactg ccggccgctg cgggctctac ggttgttttt gttcgggtca cggtcgctgg
     8281 agctaagatc actcgcgggg cacacaggaa cggattccgg cgcaaactcc ggaactccca
     8341 ttacgcggcc gcgccttgtt ccttgttgaa ttcggcgaag gtcttgccct gctcggccaa
     8401 ttgcttcagc agcggagcag gctcccagag ctcgccatgc tcccggcgaa actcgcaaat
     8461 gcgctcgtac actttccgca gcccaaccgt atccgcatac cacatcggac cgccgcgata
     8521 ggacggaaac gcgtagccgt agaggtaaat tacgtcaata tccacggcgc gcagcgcgta
     8581 gccctcttcg aggattcgtg ctccttcatt caccagcgcg taaacgcagc ggtcaacgat
     8641 ctcttcggcc gcgatctttc gttgtggaat cccggcctct ccggcccact tccggacgag
     8701 ctgctccacc tcgggatccg ggatggcacg ccgctgttcg tcgtacttgt accaaccagc
     8761 gcccgtcttc tggccatagc gccccatttc acagagccga tcctctgcga tcggctgcct
     8821 tacgccgggc ttttgcaggt ggcgatactc cttgcgtatg cgccagccga cgtcgaggcc
     8881 ggccaggtcg ccggtcgcca gcggccccat tgccatgccg tattcataca gcgcgctgtc
     8941 caccgcttcc actccggccc cctcttcgat gaggaactgg gcctcacggc ggtacgggcc
     9001 gaacatgcgg ttgcccacga agccggtgca attgcccacc aacacgccga ctttgccgag
     9061 ccgcttcgaa agctgcatgc acgtcgcgat cacttctttc ccggtcgctt tgccgcgcac
     9121 gatttccagg aggcgcataa cgttcgcggg gctgaagaaa tgcgtgccga tcaccgcgcc
     9181 cggccgcgaa gtggccgagg cgatttcgtc aatgttcagc gttgatgtgt tgctcgcaag
     9241 tatcgccccg gtcttgcaga cgcggtcgag ctggccaaac acctctttct tgagcgccat
     9301 gccttcgaac accgcctcga cgaccatgtc cgcgccgcca aatccttcgt aggtaagcgt
     9361 cggccggatg agcttcaagc gttccgccgc gaacgcctcc gtgaagcgcc ctcgttttac
     9421 cgaatttgag tagttcttct ggatgttcga gagtccgcgg tcgagcgccg cttgatcggc
     9481 ttctttgagc aggacgggga tgccggcgtt ggcgaacacc atggcgatgc ccgcgcccat
     9541 ggttcccgcg cccacaacag ccgcgctatt caccggaaga accggcgtct ccttcggcac
     9601 gtcaggaatt ttcgccacct cgcgctcgcc gaaaaagacg tgaatgagcg ctttggactg
     9661 atccgagaga aggcattgca tgaagagctt ccgctcggcc tcgcatccgg cttcgaaggc
     9721 cagttttgtt ccggcttcga tggcgtcaat ggcggccagc ggcgcgatcg tgccacgctg
     9781 ttttttccgg gccgtttcgc gcgcggcggc gaagattggg gcgttctgct ccggcatccc
     9841 cagcttctgg tttcgttcgc gcgttttggg agccggcttt cccgcaatct cccgcgcaaa
     9901 ggcaaccgcg ccggccagca agtcaccctc aatgattctg tctacgatct cgaacttcac
     9961 cgcctcttcc gccttgatgg gatttccttc ggcgcacatc tcgacggcct tcgcgacgcc
    10021 cgccaggcgc ggcaggcgct gtgttccccc cgcgccggga atgatgccga gcttcacttc
    10081 cggctggccg acttgcgcgc cgggcgcggc gaccctgtag tgccccgcca tggccagttc
    10141 gaggccgcct ccaaacgccg tgccgtggat cgccaccacc atcggcttgc ggcaatcttc
    10201 gatgcgcaac agcaaaggaa gaagccctgg gccgcgcgat ttgcccgcgg tcatctttcc
    10261 aaattccttg atgtccgctc cggcgacaaa cgtgcgtccg ccgccgataa gcacagccgc
    10321 gcggacggtt tcgtccccgt cgatctgctc gatcgccgcc gcgatgccct ccggcacccc
    10381 ggggctcagc gcattcaccg gcggattgtt gatcgtgatg acggcaatgc cgttttccgt
    10441 ggaaagttga atgaggtcgc tcatgtccgt ggccctcttt cgttcgtgaa ttacgcgctg
    10501 cgtgccgccg ccgcgcccgc gtcaatcacc atgcgcgcga gacgctcgcg gtgaaaggtc
    10561 gcatcgccca gcgccgtttc cgaagacttc gcgcggcgat aaaacaaatg caaatcgttt
    10621 tcccaggtga agcccatgcc gccatggatc tgaatcccgc ggttccccac ggtgcggctc
    10681 gcatcgctcg cgtacatctt ggcgatcgag acggccgcgg cggcgtccgc aacgttctcc
    10741 tccagcgccc agccggcgta gtaagccgcc gagcgcgagc tttcggtttc caggtacatg
    10801 tccgcgcacg gatgctgcac cgcctggaag gtgccgatcg gcttgccgaa ctgcttgcgc
    10861 gtcttcgcgt attcgaccgt gagatcgagc gtgcgctgca tcccgccgac catctccgcg
    10921 acgagcgcgg cggtcgcaat ctgcagtccc ctggccaggc cggcttcgtg gcccagtctc
    10981 tccgccggcg tgtcttgaaa ctccacggaa taaagtttgc gcgtcaaatc catggccggc
    11041 atggccgtcg tgcgaattcc gtgcgcccgg ccatccacga tgaaaacgcc gttgtgcgcg
    11101 acgacgatca ggaaatccgc cacggcagca tcggagacga aaagtttctc accggtgagc
    11161 ttcccgttca ccacggtgat ctccgcgtcg gcagggtccc agctcgcgct cgattcgaga
    11221 atggccacgg ttgaccgcgc ctcgccctgg cagatgggcg cgaggtattt ctttttctgc
    11281 tcggccgagc cggccgagtc caggacggcc ccgcccagcg ccacggtcga aaagaagggc
    11341 cccgggagaa gcgcgcgtcc cgcctcttcc atcaggagaa tgagttccac cttgccgagt
    11401 cccgcgccgc cgtattcttc cggaaaaatc acgcccgtgt agccctgtgc ggcgagtttc
    11461 gcccacaaag ccgcatcata ggctgtctcc gtttccatca cacggcgaac ttcggccatc
    11521 ggacattcgc cggtgaaaaa cttgcgcgcg ctgtctttca ggatctcctg gctctcactc
    11581 aatccgaatt gcatggcctc tcctcttcgt gccgttcgaa cttcatggag gtaaatcgag
    11641 cctggcgccc gccgagggcg acggcagttt ggcttgacga attcttgcac gcatcaagcg
    11701 ggaacggcat tagtagctcc tcggcaagcc gagcacgaag tgcccgatga tgttgcgctg
    11761 gatctccgat gtgccggctt cgattgtgtt gcctcgcgcg cgcaagtatc cgtaggacca
    11821 catgccgttg tctacggcgt gatcgccgga tccctcaagc tgcccgtacg gtccgagcag
    11881 ttcctgcgcg atctgctgaa ggcgctggtt cagttcgctc cagaaaatct tcaggatgga
    11941 tccctcgggg ccgggcactc ccgtggcgct gatgcggctg aacgcgcgca tctggttcag
    12001 ccgcatgatt tcgatttcgg cgtagcattg cgcgagtttc tgccgcagga gcgggtcctc
    12061 cgcggcaggc cgcccgttgc gacggacggt gcgggagagt tcgatcaggc ggtccatgtt
    12121 ccgcttgtaa ataatctgca ggccggcgcc gaaggagccc cgctcgtgca tgagcgtgcc
    12181 catcgccact tcccagcccc ggttcacgcc ccccagcacg ttttctgccg gcacgcgaac
    12241 atcgcggaaa aacagctcgc tgaactcagt ctccccggtc atctgccgca agggccggac
    12301 gtccacgccg ggcgtcttca tgtccagcag aagtaccgtc aggcccttgt gcttcggaac
    12361 attcgggtcc gtgcggacca ccacttcgca ccagtctccc acccagccgt agcttgtcca
    12421 aaccttttga ccgttgacga tgtaatggtc gccgtcaagg cgcgcttcgg tctgcaagtt
    12481 cgccagatcg gagccggcgt tcggttcgga aaatccctgg caccagattt cttcggcgct
    12541 caggatcttg cgcaagaaac gcttcttctg cgcttcgctg ccgaacgcaa tgatggtggg
    12601 gccgatcagt cccaggccca ggacgttggc catcggcggc gctccggccc gggccatctc
    12661 ctgccagaaa agaacctgct ccatcaggtt cgcgccccga ccgccgtact cttttggcca
    12721 cgaaatcccg gcccagcccg cttcgtacag cttgcgctgc caggctctca tgaattcgaa
    12781 acggtgatcg agagacttct cgcgctgctt gtcccagtct ttcggcgcgt tcgcggcaag
    12841 ccacgcgtgc aatccgtcgc ggaactccaa ttcttcagcg gtcagattca ggtccatctc
    12901 gagcgcccct ttccctcaac cctgtcaggc ggactgccct ccatccacga tcagcacgtg
    12961 acccgtgacg taaccggagg cgtccgaggc caggaagaca gccgctccct tcaaatcata
    13021 gtcgtttcca aaccggccga gcgggacctt cttgagaaac aaatctcgat tccgttcgat
    13081 caaaacttcc gacatgtgcg tgggaaacca tcccggcgca atcgcgttca cctggatgtt
    13141 gtgcatgccc cacttgcagg caaggtcctt tgtgagggcg atgaccccgc ccttgctggc
    13201 gtggtatcca atcgcttgaa attccggcgg cgaacctcgg agcccggcca ctgaagcgat
    13261 attgatgatc tttccccggc cctggcggat catgatcttg cctgccgcct gcgagaaaag
    13321 aaacgttccg gtaagatttg tctcaatgac tttgttccag tgctccagcc gcatctcctc
    13381 cacgggagcg ccccaggaag tacccgcgtt gttgatcagg atatcgatcc tgccgaattc
    13441 ggagacggtt gcgtctacga cctcctgaat ttgcgcaggg tttttcacgt cgcagccgag
    13501 agcgagtgtt ctcacaccaa gcttctgtaa ttcttccgcc gcttgctggc accgctcctt
    13561 tttcctggca caaagcacaa ggtttgcccc catttcggcg aggccttccg ccatctggcg
    13621 tccaagcccc atcgagccgc cgctgatgat ggctacgcgt ccggttagat caaataattg
    13681 tttgacgttc atcggcccgc cgaatcctcc cggattcgca acactctatc gcagaatggt
    13741 gtgcattggc ttccgcctcg gtattgggtt cgcagcgcag gagcaagcca aaaaccgacg
    13801 cgcgcaagga gccacgcctc gcctcacgca aatgccgccg ccatcggtga ggcgtgagga
    13861 gaaaccgctt gctctctcgg ccggcgcgac gcactaggct acaccgagag gctgatcatt
    13921 cccagaccga gttgccggcg aaagggtccg catctcctgg ccgggtggaa tggaaacgat
    13981 tgggagccgc tcaggaaata tccggcagag gctgggaact ccgaatgtgt gcggcaggtt
    14041 ccgcagtgga ggatagcagg cacagggaaa atagtgggaa caggctgcaa ggcgtagaat
    14101 gagggggagt tccggtttgt ggaggtctct ccgatgaaaa aataccgcag cctgcattgg
    14161 accgtcatgc tcgcgttcgc tgttccggtt ggtggcctgg tgttcggggg tctggcgctg
    14221 gcgcagcgca cgctcgaaaa aacgctaatc gtaaatggaa aaccggtggc aggtgcgatg
    14281 gcggatatgc agggccgcac ttatgttgac ctccagatgc tatcgcagac actgggcgga
    14341 acgctgacgt ttgagacgga ccggattacg cttgtgattc ccgcggcggg cgcgggcaac
    14401 tcgcagcaaa cgcgtccgca ggcgcccacg agccagccgc cgacgcccga gcatctctcg
    14461 acgaatttca ggacggccgc gatcaccgcg ctcggcgaga tgcgccagtg gcaaggggcc
    14521 gtcgaaagcg tgatcaccta tggcatcccg gtggcgggct cgtggccgca ggattaccgc
    14581 gacagggcgg agtccggaat caaccaggca aaggtggttg cctcgacgga cgaagaccgc
    14641 agcgccctcc tgcttctgga gaatgatttt gccagtttga ggaattggtc ggacacagtc
    14701 gtcacggaga gaaagagtct gcatggcgcg cggttcgtag atccagatgc gctgaaaaag
    14761 gatacggccc tggcgaagat taccgactgc agccggtttc tgggaggcat gattagcggc
    14821 ggcgtttact cggataacgc gagctgccat tgagtgagga cagaaccggg aacctgaatt
    14881 acacaccgcc aacgaagtac cccacgttcg cccgttcggg ccgaacattg ggcacccaga
    14941 ttcgtatcac tcgactaagt ttggggcacc cgtcctggct cagcacacgc tcgaaaaaaa
    15001 cgctgatcgt aaaatggcca gttcccatga actcgtcttc aagcgcacaa gttcctggtt
    15061 cagcaggaaa tcaacaaacg tgattttccg ccgcgccatt gaggacaggg cggcttgagt
    15121 cagtcgtgca gcacgacgct caagagagaa gtgttgatct gaagagagcc gttgtacttg
    15181 atgaagccca atttcctgaa cttgttcatg aaaaaattga cccgtgagcg cgttgtgccg
    15241 accatctccg ccagcgtttc ttgcgatact ttggggagtg ttccatgggg cttgtcgtct
    15301 ttaccgtaac gggcaagcaa tagaagagcg cgggcgagcc gcttctcgct cgagttgaat
    15361 agctgatcga ccaagtcctc ctcgatgcga atgttccgtg ccagcatgaa agtgatgaac
    15421 cggtcggaga gggcatgctg ctcgtgaaga accttgaaca tttcactctt ctcaatgacc
    15481 agcacagtgg tgggcgtaat tgcggtaacc gtgcccatgc gaacggactg acctgccaga
    15541 cagccttcac caaagaagtc gcccggtccc agaatggcga cgaccgcctc tttgccgatt
    15601 tcactaacga cggagagctt cacgccgcct tcttgtaagt acattacgtt cttagcggga
    15661 tcaccttgag tgtagattac ctccgctctc ttgaactctt tcacttttct cgccaccccg
    15721 gccgaatcga gaaaagcttg ggcgttaaat gcgccttttt tctcagaaat accattggat
    15781 tttttgttgc tggaactcat agctccttcc ataacgcccc tcctattaaa aacctagcaa
    15841 aaccattcac ctcaagtcaa gactctcttg cgtagcggca atgtcctagt taagtgtcgc
    15901 gtgtcgcaac tttttcgaaa gctcgccctc gaaataacct tcaacctcct catggctaag
    15961 cggggttttt actttggtca aaacgatcga ataaacgacc ttgttccttt caacctgcgc
    16021 ggtgataaac gcctgggcct ctagcatttc ctggttggtg aatttcaccc ttctgccgtc
    16081 tgcaagtgtt ttaatccact cttcgtccac catttttctc ctcaggcggg tccagcgttt
    16141 tttgtagtag ataccccatg ctccaagcag ggtctgccaa tgctgcctag atcgccagag
    16201 taatatctac ccttagacac catggttgat aacactgaaa tgggggcact cccagtagag
    16261 ccgctacttt cgactagact tggacatgct cgtttcatcg cgacattcct aatttgttat
    16321 tttttcttga taaacttggt tcgcccccta ggtctgaccc ggcgcttgac tgtatgacgg
    16381 atactatcct gacgggggtt caggcccaac ttctccacaa agcgggctag atcggattcg
    16441 acagtacgga gagtgatctt cagtcgggcg ccaatatcgc aactgcttgc cccctccaag
    16501 acgagttgca acacctttcg ctcgcgggtt gtcagtgtgt cagaaagttt cagcaaagca
    16561 gtctgaaact tttgcaaata tctgcgagtg gaagcctcgg aaagcagtgg actcaagtaa
    16621 cgtcgaccgg caaccacatc gcgtatggct tggaagaggt cagctccaca ggactctttg
    16681 acgacaaaac cagccgcacc gcttccgagt gcatccaata aatacacctc gtctccatac
    16741 atagtaagaa taaccacacg agtatctaat ttcaggcgcg agaccctgcg tgtgatttcc
    16801 agaccgtcca ggccgggcat catcagatcg gcgattagca ccttgggctt ccggctcttg
    16861 acaagttcga gcgcctcgat cccgtcagcc gcttcgccca caacgacaaa ttccggatct
    16921 gattccagca agaggcgcag cccttgcctc acgacaacat ggtcatcagc caacgcgacg
    16981 gtcaccatga cgcctttcaa gaaccttgag ccctctttca ttcagagacg cgccacaacg
    17041 aacagtggcc tgtcacatcg ggctctgcgt ttggagccga ctccatcaga acttagccct
    17101 taaaccctag aatatcatgg agctttgtag aaaaccgacg actttctcgt agagcattga
    17161 cggccattct ttgtttcccg gagccgggac caagtccttg accgcaccgt aacttagctt
    17221 gagtaggaaa ggctgtctct cgttcttact ccaggattct ctgtcgaata gagattgcgc
    17281 caaccacact cgagcatggt caagccccaa tacaaattat gtttttctgt ttgcctggga
    17341 taggattgcg gaagagaggc ataccagtgg cgttccgctg gtgcttgcac tgacagtttc
    17401 gagattaatc tggaataaag gacggaccgc cgtggccaca gcaaaacgaa gggcaaaagg
    17461 gagagaaaca ggaggaccgc agagaaaaag gaaagagctt ccgaaggtgt cgacaggcat
    17521 tgccggattg gatttggtgt taggcggcgg cctgcccgcc ggacgcgtga cccttctcag
    17581 cggtggtccc gggagcggca aaaccataat gggcttgcaa tacctgctac acggcgccgg
    17641 tgccgaccag ccgggcattc tgcttatgtt cgaggaacgc gccgcggccg tgcgtcaaaa
    17701 cgcttggagt cggggatggg acatggttcg gctagagaag aaaaacaagc tctacctgat
    17761 ggatgcgcgt ttggatcctg aagcggtaat ctccggcgag ttctccatca gtggtctgct
    17821 ggctatcctt gatcagcgga tcaaggccat gcgcgccaag cttatcttaa ttgacgcggt
    17881 ggacgcgctc ttgcatttgt acgataaccc catgcgcgag cgtcgcgaat tatatgcgct
    17941 gcacgaatgg ctgctggacc ggggattgac gacgatcatg acggtcaaaa ctgtcccgca
    18001 ggaggaggtt cctgcccggt atgcatttct ggacttcatg gcggattgtg tgattcacgt
    18061 ggaccagcgc gtcactgctc agatcaccac gcgccgcttg agagtgatca agtatcgtgg
    18121 ttcgggatat ggccgcaatg aatatccctt cattatcgat gagaacggca tcaacatcat
    18181 tccgatcacc agcaacgttc tacagcaccg cccccccggt cccaaagtgt ccagcggcca
    18241 ggcttggctg gatgaggtgc tggtcggcgg ctacaagtgt gggaccagca tcctgatcgc
    18301 ggggaccgcc ggcgcaggaa agaccacact ggcctgcgtc ttcacccggg ccgcttgcct
    18361 gcgcggcgag cgagtgcttt atctgaattt cgaggagtcg gcagaatcca tggtcagcaa
    18421 catgcttagc ccaggtcttg ccctacagcc gctgatcaag acgaggacgt tagtggtccg
    18481 gtcctacttg ccggaagcga tgggtgtgga ggagcactta tttcatgccc tgaaaaacct
    18541 agatgaggtt caaccccagc acgtggtcgt ggatgcgatc tcggcgtgca agcgcatggg
    18601 ctcggaccag gctgcctttg aatacctgat gcggattctg aacgcctgta aagagcgcgg
    18661 caccacctgt atctatatca accagacttc gggtttggat atcgtcgaag agatcagcgg
    18721 gatcggcatt tcgtccatca tcgataccat cgtccttcta cgccatcttc cgatcggtgg
    18781 cgccatagcg cgccagttga tcgtcatgaa gtcaagaggg tcgaagcatt ctgaacagtt
    18841 ccatgaattc cgcatcaccg atcgagggat tgaccttgtg aaggcctagc gtagttttgc
    18901 ggtgagccta tcggcgtatc cgacaggggt acggaagaaa tggagcccga tgatgaaagc
    18961 tcgcaagcgc gagaggcgaa gacacgcaaa acctggaggt gagacctatg tcttccggct
    19021 gtttgtggcc ggtaacgaat caaattcctc gcaagccagg ggaaatttgg ctcggctctg
    19081 cgaggagcac atcaagggac gttacaaaat cgacgccgtg gacgtactga aagacgccgc
    19141 tgccgctcac aaaaataacg tgctggtgac gcccatgctg atcctggtca agcccctacc
    19201 gaaggtcaca gtgttgggca acctcaatga taccaagcag gtccttgccg cactcagact
    19261 gaccggtgac gggcgatgag tgaccagaaa ccgccatacc gcgaactaga gaccaagatg
    19321 aaagaggctg aggaactcat ccacgccctg aggacccagc aggtggacgc gattgtcggt
    19381 aagcgtcaca tcatgcttgt tcgtttgaag caggcggaag agaacctcaa aagctcgagg
    19441 aatcaattgc gtgccctcgc ggcatacttg ctatcggtgc gcgagaatga aagggccgtg
    19501 atcgctcgtg agcttcacga tgagtttggc caggcgctca ccagcttgca cttgggattg
    19561 gcctggatat cccgcaagat gactccaggg cagcagccgt tgcaggagaa aatcagatcg
    19621 ctatcagaca ccacgacgag cttgatccgg tcggtgaaga acatcgcgag tgagttgcga
    19681 cccggcgctt tggacgaatt gggcctggtt aagaccttga aatcggaggc gcgggaattc
    19741 gagggacaca ctggaataca gtgcaggttt aagacgaata ctgcaaaagc caagtttgac
    19801 cgttcagcgg cggtcgctat tttcaggatc gtccaggcag ctcttacgaa tgttgcccgg
    19861 catgctcatg cttcgcgggc ccttgttact ttgatgaaga agaaaaacgg gctgatcctg
    19921 gcggtgacgg acaacgggaa gggcataacc aggaagctga tctatagcca tgattcgctt
    19981 ggcattaccg gcatgcgaga gcgcgccctt gcgcttgggg gtacttttgc actcggaggc
    20041 tcaagaagca aggggacaat attgacagta cagattccgt ggtcccgggt cactatcggg
    20101 acagccccct agtccctccc gcaacaactc cgcagtctga ttcgcgataa gaccaaggaa
    20161 gctcgacccg cgcaaataag caggccggac attcgtgtgt ttgggggaag ccgccgtcgc
    20221 gaaatcgatc agaagaacgc atggattctc gcggttcgat cgccggtt
//
LOCUS       DASYVO010000093         1776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_30311, whole genome shotgun sequence.
ACCESSION   DASYVO010000093 DASYVO010000000
VERSION     DASYVO010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30311"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..>1776
                     /locus_tag="VGR03_02935"
     CDS             149..>1776
                     /locus_tag="VGR03_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV1993264.1"
                     /translation="MIATSNVLTFTGVFLGAGLFALLSGPAQLQPHQVLLVMAAISFA
                     ATVYVMTVLPDFTIRLCLWLLTHTFYRIQVRGAENLPRRGPALLVCNHISYVDPFLIG
                     ACTQRFIRFLMYRRFYESFGVHGLAKLMGAIPIAEDDPPRRVVESLRAAQAGLQEGHL
                     VCIFAEGAISRTGKLLRFRRGFERITRGLDVPVIPIHLDRVWGSIFSYERGKFFFKWP
                     RRIPYPVTISIGPPLSGGSTAFEVRQAVMKLSADAFARRDATQRPLPELFLGTARRNW
                     RRFAMADSFGRRLNFGRTLVGAMLFRRMILDRCGAEKMVGVLLPPSVPTALLNLGITL
                     AGKVPVNLNYTASAEAMATAINRCEIKTIFTTEKLLDRLAVPRSPGMVMIEDVAKGFS
                     PASKYAYTVAARLLPRFVLHAWLLSPGQSLDSLATVIFSSGSTGIPKGVMLSHRNIVS
                     NVEGVQEAIAVDRNDCLLGILPFFHSFGFTDGLWLPMLSGFGVVYHASPLEARKIGEL
                     CRKFAVTILISTPTFSWKYVQLCAAEDFATLRLAV"
BASE COUNT          292 a          531 c          499 g          454 t
ORIGIN      
        1 cgtattcgga gtttaccttt tcttcgccca ctcctcttac cctcatgccc tgatcggcca
       61 cttcttgctg ggcttctcag gcggaatatt tatcatcccg cttcaatctt acttgcaagc
      121 gaatgccggc gagcattcca agggccggat gatcgcgacc tccaacgttt tgactttcac
      181 cggcgtcttc ctgggcgccg gcctctttgc gctgctttcc ggccccgcgc agttgcagcc
      241 gcaccaagtg ctactggtga tggcggcgat ttcgtttgcc gccacggttt acgtcatgac
      301 cgtgcttccg gatttcacca tccggttatg tctatggctg cttacccaca ctttttatcg
      361 tattcaggtt cgtggcgcgg aaaaccttcc ccggcgcgga ccggcgctgc tcgtttgcaa
      421 tcatatttcc tatgtcgatc cctttctgat cggtgcgtgc acgcagcgct tcattcgctt
      481 tctgatgtat cgccgatttt acgaaagctt cggcgtgcat gggctggcga aactgatggg
      541 cgcaatccct attgccgagg acgatccgcc ccggcgcgtt gtggaatcgc tgcgcgcggc
      601 gcaagcgggt ttgcaagaag ggcatctggt ctgcattttt gccgagggcg cgattagccg
      661 caccgggaag ttgctgcgct ttcgccgcgg tttcgagcgc attacgcgcg gccttgacgt
      721 tcccgttatt cccatccatc tcgatcgcgt ctggggcagc atttttagct acgaacgcgg
      781 gaagtttttc ttcaagtggc cgcgtcgcat tccttacccg gtgactatta gcattggccc
      841 tccgctttca ggaggctcaa ccgccttcga agttcgccag gcggtgatga agttgagtgc
      901 tgacgccttc gcgcgccgcg acgccaccca gcgtcccttg cccgagctgt ttttgggcac
      961 agcgcggcgc aactggcggc ggtttgccat ggcggattct ttcggtcggc gcttgaattt
     1021 tggacgcacc ctggtgggag cgatgctgtt tcgccgcatg atcctggacc ggtgtggcgc
     1081 tgaaaagatg gtgggagttc tattgccgcc ctcagtgcca acggcgctct tgaaccttgg
     1141 catcactttg gcgggaaagg tgccggtgaa tctgaattac actgcttccg ccgaagcgat
     1201 ggccaccgcg attaatcgtt gcgagattaa aacgattttc accacggaaa aactgctcga
     1261 ccggctcgct gttccccgaa gccccggcat ggtgatgatc gaggacgtcg ccaagggctt
     1321 ttccccggca agtaaatacg catacaccgt cgccgcgcgt ttattgccgc gtttcgtatt
     1381 gcacgcctgg ctgctgtcac ccggccaaag ccttgattca ctcgctacgg ttattttctc
     1441 gagcggcagc accggcatcc ccaagggcgt gatgctgagt catcgcaata ttgtctcgaa
     1501 cgtcgagggc gttcaggagg ccatcgccgt cgatcgcaac gattgcctgt tgggcatact
     1561 tcctttcttc cactcgttcg gctttaccga cgggctctgg ctgcccatgc tttccggctt
     1621 cggagtggtg tatcacgcca gtccgctcga agcgcgcaaa attggtgagc tgtgccgcaa
     1681 attcgccgtg acgattttga tcagcactcc gacattttcc tggaagtacg tgcagctctg
     1741 cgcggccgag gatttcgcga cactgaggtt agcggt
//
LOCUS       DASYVO010000094        23648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_30335, whole genome shotgun sequence.
ACCESSION   DASYVO010000094 DASYVO010000000
VERSION     DASYVO010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 23648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23648
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30335"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VGR03_02940"
     CDS             complement(<1..548)
                     /locus_tag="VGR03_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993265.1"
                     /translation="MRRSDLVQHNEREKGAATRTSQIVFGERQHLLRVLDSLEGTDLP
                     IARMQQERRILEELIHARTRDLNQINTAWDEKIGLVLSSDAKPEMLEKLVKQAPEEDF
                     YLLRLISEHPRANAKTLNKLAKHPYGAIRENVARHPNADASTLTWLSRDRSQPLWYLV
                     AFNPNTPTPLQRRLRDRLKRLG"
     gene            774..1046
                     /locus_tag="VGR03_02945"
     CDS             774..1046
                     /locus_tag="VGR03_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993266.1"
                     /translation="MMSAFQEHREELEHYEQMFGRDRGRLAVSLDRLTNALVLVGQHG
                     VYCTSQRNPAVPAMDLRIINQELVHAKELVQSVMEEMRLAKQKSES"
     gene            complement(1112..1948)
                     /locus_tag="VGR03_02950"
     CDS             complement(1112..1948)
                     /locus_tag="VGR03_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1993267.1"
                     /translation="MSNGRPTLNAATEGLIHITIPVGMLQCNCSIIGDPATREALVVD
                     PGDEVNRILDLLGRHKLIVKAIVSTHAHIDHVGGLSKLHQYTGAPVLMHRDDLPLYNA
                     MDMQAAFLGVLPPELTDIDQLLKEGDVLRWGRFEAQVIHTPGHTPGSVSLYLPDDAGK
                     VTVAAVPKKITVKDEAGDEIAIADLMKKITDPDDSREMAVPDESETIVPRAPQLFSGD
                     TLFAGSIGRTDLWGGSMEQIMDSLRSKLMHLPDETIVYPGHGPASTIGDERHLNPFLQ
                     PE"
     gene            2036..3127
                     /locus_tag="VGR03_02955"
     CDS             2036..3127
                     /locus_tag="VGR03_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993268.1"
                     /translation="MRERAEMATPGLALNTPLAALHLAAGAKMGVWFGCALPDYFGDA
                     AAEYRNARDTVALIDKNYRAYLSFAGPDRVRYLNAILTNNIKDLRAGHGIVSLLLNPQ
                     GHILAEIETYAFADRLFCVSHVMIRERLVEWLDKFIIMDDVTLTDETPRYGTLALEGP
                     KAAAIVKEVSGADVTKFEELSSHGGTVGSIPCYIVKRSPGGVPGAEFVAESEKLAELW
                     QILSDAARWHEGGPMGYAALSATRLAQGVPWFGYDFGEKQIPHEAGLQDSHISYTKGC
                     YTGQEIVERVRSRGQVNRRRVELLFSGDAVPVPEALLTLDGKEVGYATRAARTWDPPR
                     IIGMGYARKEANAPGSVLQCAGGTATVTR"
     gene            4108..4527
                     /locus_tag="VGR03_02960"
     CDS             4108..4527
                     /locus_tag="VGR03_02960"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993269.1"
                     /translation="MPTMKEFTIRLEDRPETLGQLCQALADQSVNILAFQSFPIENGK
                     SSVRLVLDNPVAAKKILDGRRTDYTETEVAQVRLANRPGELARAASRLGLAGININYG
                     YCGAGPDAKATFLIFGVAEAGKAAKVLDQPAAAGAAV"
     gene            4700..5137
                     /locus_tag="VGR03_02965"
     CDS             4700..5137
                     /locus_tag="VGR03_02965"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEV1993270.1"
                     /translation="MWNKKSTDAMSPLDATADRETGWLGSSLHVKGDISGTEDLLIDG
                     SVEGLIQLEERKLTVGTTAKVTADINARDVVVYGYVKGNVRAKGRIEIKKDGSVIGNL
                     TTAQIMIEDGADFKGSIEIDRSAVEQADKNVSSRAAAAGAGPI"
     gene            complement(5324..6160)
                     /locus_tag="VGR03_02970"
     CDS             complement(5324..6160)
                     /locus_tag="VGR03_02970"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1993271.1"
                     /translation="MRTLAHLSDLHFGRIEPATVQALLATVTEARPDVVVVSGDLTQR
                     AKEREFQEARRFLDALPSPQIVVPGNHDVPLYNVLARTLKPLRNYQRYITQDLEPFYF
                     DDEITIVGINTARSLTFKSGRINRRQVARSCARLEACGENRTRIIISHHPFDLPESRE
                     LHGLVGRAHMAMAGFARCRVDLILSGHLHISHTSESAPRYKIPGHSALVIHAGTATSS
                     RARGELNSFNILQVDRSSVSIQCLTWNHERGSFLLSATAQFHRTPAGWSRIPPADAAP
                     KP"
     gene            complement(6176..7093)
                     /locus_tag="VGR03_02975"
     CDS             complement(6176..7093)
                     /locus_tag="VGR03_02975"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEV1993272.1"
                     /translation="MASSGLTCILNCASGSCSGPELREQLSRIFAEIGTPARILLAPS
                     GAQVSELARRAVQENSKIIVAGGGDGTLNAVATELLGTSIPLGVLPLGTWNHFAKDLR
                     IPQDLDAAARNLFTGRAVPLDVGEVNGRIFLNNSSLGIYPRMVRRREEQQKQGCHKWL
                     AFAQASLSVLRHYSLLRVRLCLEGRELLRKTPFVFIGNNQYEMEGLNIGGRQRLDAGE
                     LCLFVANRAGRMDLVRLAFLAFFGRLNEASDLDVLTAKQFSIETRRPHASVSTDGEVH
                     QMNTPLNYLIRPGVLRVIVPVENVPENSA"
     gene            complement(7466..7627)
                     /locus_tag="VGR03_02980"
     CDS             complement(7466..7627)
                     /locus_tag="VGR03_02980"
                     /inference="COORDINATES: protein motif:HMM:NF033488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEV1993273.1"
                     /translation="MRISAFLALFMVLLLLWAGGFLVYHVTSFLIHLLLLFAVISLVI
                     HLFRGVSGK"
     gene            complement(7753..8376)
                     /locus_tag="VGR03_02985"
     CDS             complement(7753..8376)
                     /locus_tag="VGR03_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993274.1"
                     /translation="MNSSEKTSLPFMVKIQVFLAVLATLVTIALASYIPTLVQRKSLL
                     DSEIERLNTQRAQLQNQIADLQKQKAEADKLGSAVASALYASNPKQAQKTIEDSLNSN
                     PEASVHPRIFIHIRSASQRPAARLVADDFRQNGYVVPGIQILVEAGPDETQARYFHAA
                     DESEAQKILGQLSASGVKNVAAKPNFIAGHDDIRQRQYEIWFAPNSL"
     gene            complement(8381..8635)
                     /locus_tag="VGR03_02990"
     CDS             complement(8381..8635)
                     /locus_tag="VGR03_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993275.1"
                     /translation="MAKKWSPADIQKVMAVSSASSCMVILVGSVSIGVVTGRISPDLL
                     GSVKGLGVGGGLVGLALVLYMVIRVALKPDVKDPEPAGDA"
     gene            complement(8762..9142)
                     /locus_tag="VGR03_02995"
     CDS             complement(8762..9142)
                     /locus_tag="VGR03_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1993276.1"
                     /translation="MVISKRLRAIREQKDLSQGDIEKRTGLKRCYVSRVENGHTVPSI
                     ETLEKMARALEVPMYQLFYDGEVPPEFRSLPKGISANSDEWGSSGESARFFQQLRQIL
                     GLLSEADRKLIMHMTFQLVRKNRP"
     gene            complement(9266..10069)
                     /locus_tag="VGR03_03000"
     CDS             complement(9266..10069)
                     /locus_tag="VGR03_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993277.1"
                     /translation="MKLRVVLYCLLGGLPVTIAALGAGHFVWWWLSGILFAASFVPVA
                     LFGPKKAPGQFGVIIPVLLIVTMLCTWSEALIFVPGYSQHAGRDLFGSVVMYLIVAAV
                     LALLAASLKMPKPGEPDSQHRTSVSAAFMILVSGLSYLFYYLIFGGITYQFFTKGYYP
                     EATQAVARLGLWFWVIQFARGVLMTFAVVPIIYTLRMKRWHAAVAVGSIIWITGGAGP
                     LLVPNGLMGTTQRFIYIVEIFTQNASLGVTAVLLLRPKSVSDAANPPAA"
     gene            complement(10066..10629)
                     /locus_tag="VGR03_03005"
     CDS             complement(10066..10629)
                     /locus_tag="VGR03_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1993278.1"
                     /translation="MKDTSPLAFALLGLLQQKPRSGYDLRKIFSNTPMGSFSDSPGAI
                     YPALQRLEKRGFVACRVQVTSGLRRRRLFRPTAAGRAAFKAWQTRAIASDDVINHVDE
                     LMLRFSFMDETAGSVQAMRFLNALAKELVAYIPSLREFLKVQGASIPLSGRLALESGI
                     RGYETLLGWTRSALATYRKRRKEGARR"
     gene            complement(10739..11227)
                     /locus_tag="VGR03_03010"
     CDS             complement(10739..11227)
                     /locus_tag="VGR03_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993279.1"
                     /translation="MTPSADPARQLLRHTVATVAYRAGKALRGAPDHFASFHIGDKTR
                     TPAQILAHMGDLFDWALSIAQGQQAWHDSTPLPWSAEIERFFAAIKKFDDFLASAEPL
                     DGSAEGLFQGPVADALNHIGQIAMLRRLAGSPIKGENYFKADIAAGRIGLEQSAPRRE
                     FE"
     gene            complement(11374..12243)
                     /locus_tag="VGR03_03015"
     CDS             complement(11374..12243)
                     /locus_tag="VGR03_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEV1993280.1"
                     /translation="MKTRFPVHPAALVPIPLAAALLLFSCGGGATLQPPPATGFTLVW
                     SDEFNGADGSSPDASKWTYDTGVGGNGWGNNELETYTNRTKNAQIQGGNLVITAAKET
                     YADPSDSVTRNYTSARLKTQGLFSQAYGRFEARIKIPAGQGMWPAFWMLGNNISSVSW
                     PKCGEVDIMENIGKEPGTVHGSLHGPSTVSQTSDATAAFSLPASQKFADDFHLYAVEW
                     EPGTVRFYVDANLYGTFTQSQWSAGGTWTFDHPFFIILNVAVGGDWPGSPDNTTVFPQ
                     QMLVDYVRVYTKQ"
     gene            complement(12289..12828)
                     /locus_tag="VGR03_03020"
     CDS             complement(12289..12828)
                     /locus_tag="VGR03_03020"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEV1993281.1"
                     /translation="MSTSLGKHVTLTRPDDFGNLLEDEGIIFTTLDKAVNWARKSSIW
                     PLGFGLACCAIEMMSMAAARFDLARFGAEVFRPSPRQADLMIVAGRLSQKMAPVIRQL
                     YDQMPEPKWVISMGACATSGGVFNNYAIVQGCNQVLPVDVYVPGCPPRPEAVLYAILQ
                     LQKKIDNEKGSFKRALNLS"
     gene            complement(12899..13420)
                     /locus_tag="VGR03_03025"
     CDS             complement(12899..13420)
                     /locus_tag="VGR03_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993282.1"
                     /translation="MHRSWLLVLGLVLSSPIAFGQTSSTDSQTLQALLAEVRQLRKEL
                     RTTTVAAQRVQILLYRLQIQEAAVARTERRVAEAHNELGHTRSEQKRLASEIKLRQDM
                     QSNTQNPAERKDLEELLPRLRVQLESLNEAEQQQQVKTTDAELELRTEQAKRTALQDE
                     LDLLDKSLERPNP"
     gene            complement(13478..14974)
                     /locus_tag="VGR03_03030"
     CDS             complement(13478..14974)
                     /locus_tag="VGR03_03030"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1993283.1"
                     /translation="MTEPATPSANSAATNVDVLIVGAGFAGLCMGIKLLEAGMKSFLI
                     IEKSEDIGGTWWDNRYPGCACDIPSHLYSFSFAPSTEWTRMYPGQQEIHDYLKRCVER
                     YGLTPYLRPKTRFREAIWDEAEGVWNATAGDGMRIRARVLVSGMGALHVPRYPDLMGI
                     DCFKGPAFHSATWDHSADLDGKNVAVVGTGASAIQFVPQIAPRAGKLHLFQRTPPWIV
                     PRLDFAFSEKWRRRFRSIPITRWALRQYIFCRQEIRVLGFLGNETFRKKVEAIALRHL
                     ARRIKDPKMRAALTPNYQLGCKRVLVSDDYYPTLTRSNVELVTDAIAEVRERSIVTSD
                     GVERPVDVLIYGTGFRATEPLIGCRVVGKGGVEIHEAWGARMSAYLGVTVSGFPNFFM
                     LLGPNTGLGHNSVVLMIEAQVRYAMKCLKLMNRRKQRVLEVRPEIQQSFVEEIYRRMA
                     GTVWQSGGCNSWYQDQNTGEITTLWPGSVVSYLRRTRSVSPSDYQLSS"
     gene            complement(14971..15723)
                     /locus_tag="VGR03_03035"
     CDS             complement(14971..15723)
                     /locus_tag="VGR03_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1993284.1"
                     /translation="MEGPKPGNTVLLYLHGGGYFGCSAESHRSITVFFALQGFRVFAP
                     DYRLAPENRFPAAVEDAVAVYRALLSTGYPSQRIVIAGESAGGGLALSLMLALRAASV
                     PLPAAAALFSPWTDLAATGDSIRTNTDRCAMFIGADVAASARYYLGDTDPRNPLASPL
                     YADLSGLPPLLIHVGADEVLRDDSTRLAERARAAGVPVELKIWPVVPHAWQLAPHLIP
                     EARHSLRESAAFFSTRVAPVESSKEEVRMAGA"
     gene            complement(16055..17380)
                     /locus_tag="VGR03_03040"
     CDS             complement(16055..17380)
                     /locus_tag="VGR03_03040"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEV1993285.1"
                     /translation="MWFKKSTVYVCLLTGSAMALAMAGKSSGQNSSTATEAPSGYDNQ
                     PNGLTDSASFAADRVQFEQTEFIGDGLGPIFNAQSCRECHQNPVTGSTSQVKELRAGH
                     HDAHGNFVGATATLADGTVTIVNRSLINQRAVCPAVDTITVNGQPQSFSFPQTQGQEH
                     ISTAETIRAFRMSLNTLGDGFIESIADQTLLDLASSQCSRTRGAICGEAVHVPVLEST
                     GGLAVGRFGWKDQHASLLSFSSDAYLNEMGVSNRLPPNNVDVTRICDVIPDPNNATPD
                     SEGLEDIDRFARFIRATKAPPRDTALGASSDSVTGASLFHNVGCEHCHVSSITTAPTG
                     TAVAAGAFTVPDSLGNKIIHPYSDFLLHDVGTGDGIVQTGGQETARKLRTPPLWGVRT
                     RTELLHDGRSATFFDAIQHHRGEASEVIEYFNRLNPAQQNQLITFLRSL"
     gene            complement(17558..18586)
                     /locus_tag="VGR03_03045"
     CDS             complement(17558..18586)
                     /locus_tag="VGR03_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV1993286.1"
                     /translation="MRAIRFHHHGTPDVLKYEDALDPTFGPTGVLVRVKACALNHLDL
                     WVRRGIPSVLIPLPHIPGSDVAGEIAQIGADVSTVRVGQKVVLAPGVTCGKCAACVSG
                     QDNRCRQFTNLGYMIDGGCAEFVRAPEVNCLPYPENLTFEEAASIPLVFQTAWHMLVA
                     RAELQPGEDVLILGAGSGVGSAAIQIAKFFGARVIATAGTDEKLQKAKHLGAGHLINH
                     KTQKIRDEVRRITNKRGVDVVFEHVGTATWDESFASLAAGGRLVTCGATTGYDAKVDL
                     RFLFSRQLSLLGSYMGTKSELHTVMKLVAAGHLKPVVDRVFPLAEVAAAHAYLESSSQ
                     FGKVVLTV"
     gene            complement(18626..19081)
                     /locus_tag="VGR03_03050"
     CDS             complement(18626..19081)
                     /locus_tag="VGR03_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993287.1"
                     /translation="MEHSASSTIGVALITTFGAFIGALISHFLIHRRGLDLALYKQRG
                     EAYKGLWQKTSMLSRWPQQAGVTYSQLQNLSEELRHWYYFAGGEYLSDSARKAYGNLQ
                     KALNDPTRAPNPKTLSPSEYTLLQDLCSKLRTELTHDLLSRKRMFVFSR"
     gene            19218..20054
                     /locus_tag="VGR03_03055"
     CDS             19218..20054
                     /locus_tag="VGR03_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEV1993288.1"
                     /translation="MDLFEEIVRMRRAGQRAALATIVHTNGSIPSYESSRMLVREDGS
                     IAGTIGGGCVEAEVWAAAKEVMQKEAPRKMVFNLNNEASYDNGLICGGTLEVFVEPIL
                     PQPMLYLFGGGHVSMAVAKAASAAGFGIGVVDDRESFANKERFPMAHEIYTSYEDAFE
                     KIHPNAASYVVIVTRGHKEDMRVLAWAVRTDVRYLGMIGSKRKVLSVYKALENDGYKP
                     EEFERVYAPMGLEIGALSPEEIAVSIAAELVAVRRNAASAAHKKLKYEAQASPILQTG
                     ER"
     gene            20065..20652
                     /locus_tag="VGR03_03060"
     CDS             20065..20652
                     /locus_tag="VGR03_03060"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV1993289.1"
                     /translation="MLAAVILSGGASSRMGSPKALLPFQGRPFLEHLLEVTSRPEIGA
                     RRVVLGAHAEPIAKAINLKADEIVINHEWEKGQLSSIQAALRSLPPGTDGILLCLIDH
                     PLISSALVQELIEQFYKTKSPIVLPVYEGRRGHPVIFSASLYEELLRAPLETGARAVV
                     WAHKGEIAEVRTNEEGCVLNLNDPETMNTALRQKD"
     gene            complement(20649..21017)
                     /locus_tag="VGR03_03065"
     CDS             complement(20649..21017)
                     /locus_tag="VGR03_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEV1993290.1"
                     /translation="MFSEAVLDHFRNPRNAGELPGATATVEVSNPVCGDILKLSARIV
                     DGRFAEARFLCRGCTTAIACASILTEELRGRTPAEARALTADSISQSLGGLPPATFHG
                     AQLAADAVSGLLQKLPSANR"
     gene            complement(21145..22167)
                     /locus_tag="VGR03_03070"
     CDS             complement(21145..22167)
                     /locus_tag="VGR03_03070"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEV1993291.1"
                     /translation="MRVHLVALSLILPLSTLAQTPAAPSKSEVVQYHATIDTVKYLFG
                     VASPVARLKPGNILDANSLDCFGNVLQKPGDTFALIKGDNPLTGPFYIEGADPGDTLV
                     VHILDLQVDGKQGVGAFSPGFGAINATHYTPMLEKEALPEKIWFYPIDHADHTATFQA
                     LDSNFKVKIPLHPFLGCIGVAPANGEARSSIVPAEFGGNMDAPEVSVGNILYLPVNVS
                     GALFYFGDGHAAMGDGEIAGSAIEVPMKVRLQFDLVKGKHTGWPRLENEKEIMAAGIY
                     RPVDDAVRIAFTELVGWIHADYGLSELDAYELLSKVGKIHLTEMVDPNYVVVASIEKK
                     YLPPKK"
     gene            22305..23072
                     /locus_tag="VGR03_03075"
     CDS             22305..23072
                     /locus_tag="VGR03_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1993292.1"
                     /translation="MRIVSLAPSATSILCAIGAKRALVGVTKWCADVAPVGRLPKLGD
                     CWHLQSVDELLRLKPTLVIGSVPYKKETVAKLLEQRLNFLAMNPRTLAGIEADIRLLG
                     GITQRTAAAEKVVKTMRRTFSELEKKARRWKQRARVYCEAWPNPRISSPPWVAELTHI
                     CGGEMVVPAGEKITEEQVAAARPDVIVLAWAATGSKADPKQAYKVAAWKDVPAIRNRN
                     VYVISDELLNTPGPPLMEGARALLKLFHSVNRMRERS"
     gene            23069..23527
                     /locus_tag="VGR03_03080"
     CDS             23069..23527
                     /locus_tag="VGR03_03080"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEV1993293.1"
                     /translation="MKTVVAAVIERSDRRLLIGQRRKNDTSPLKWEFPGGKVRDGESP
                     EVALARELQEELGVTLVRSVELGRVRHQYAETHDDLEIRFFAAAIAESELAPRTFEKI
                     VWALPKELGEYDFLAANRELIAQLATGRIKPAEIIESAEETGASHGPAAD"
BASE COUNT         5190 a         6102 c         7717 g         4639 t
ORIGIN      
        1 tcgccgaggc gcttcaggcg atcgcgcaat cgccgctgca acggcgtggg cgtgttggga
       61 ttgaaagcta cgagatacca gagcggctgg ctgcgatccc ggctcagcca cgtcagcgtc
      121 gaagcgtccg cattgggatg ccgtgccaca ttttcgcgaa tggccccata cggatgtttc
      181 gccagcttgt tcagcgtttt cgcattggct cgcgggtgct cgctgatcag ccgcagcaaa
      241 taaaaatcct cttccggcgc ttgcttgaca agtttttcca gcatttccgg cttggcatcc
      301 gagctcagca ctaacccgat cttttcatcc caggccgtat tgatctgatt caaatcgcgg
      361 gtacgcgcgt gaatcagctc ttccagaatc cgtcgttcct gctgcatgcg cgcgatcggc
      421 agatccgtgc cttccaggga atcgagaacg cggagaaggt gttgcctctc gccgaagaca
      481 atctgagagg tgcgcgtggc agcgcctttt tccctctcgt tgtgctgaac caggtcggaa
      541 cgtcgcattg tttggctgct ccagcccaat cccttcgcga gctcattgaa ggaccgggta
      601 ctcactacag tattcgaatc actagccaca cgaagggcag aaatcgcttc attgtggctt
      661 tagcgaatta tgttccgcgt gcgcgatgcg gtaaagtttg gtgcgagccg cgtgtgcccg
      721 atgtgcaacc gcctgctatc gcgggattgg gaagaattca aacagggagc cacatgatgt
      781 cggcatttca ggaacaccgg gaagagctgg agcattacga acaaatgttc ggacgcgatc
      841 gggggcggct ggcggtgtcg ctggaccgct tgacgaacgc gctcgtgctc gtgggccagc
      901 acggcgttta ctgcaccagc cagcggaatc cggcggttcc ggcgatggac ctgcgcatca
      961 tcaaccagga gctagttcac gcgaaggaat tggtgcaatc ggtgatggaa gagatgcgcc
     1021 tggcaaaaca aaaatccgag agctgactgg cagaactttc aatgaatagt ttttttcgag
     1081 agcttcgaga ttttccgttc tcgtccgatt cctattccgg ttgcaagaag ggattcaagt
     1141 ggcgctcgtc gccgatggtg gaggcaggcc cgtggcccgg gtagacgatg gtctcgtccg
     1201 gcaggtgcat cagttttgag cgcagtgaat ccatgatctg ctccatggag ccgccccaca
     1261 ggtcggtgcg gccgatgctg ccggcaaaaa gtgtgtctcc ggaaaaaagc tgtggcgcgc
     1321 gaggcacaat tgtttcggat tcgtcaggaa ccgccatttc tcgagagtca tcgggatcgg
     1381 ttattttttt catgagatcg gcgatggcga tttcgtcgcc agcctcatct ttaacggtaa
     1441 ttttcttcgg taccgcagcg accgtgactt ttccagcgtc atccggaaga tacagcgaaa
     1501 cgctgcccgg cgtatggccc ggagtgtgaa tgacctgagc ctcaaaccgt ccccagcgaa
     1561 gaacgtcgcc ctccttcagc aactgatcga tatcggtgag ctccggcggg aggacgccga
     1621 ggaaggcggc ctgcatgtcc atcgcattat agagaggcag gtcgtcgcgg tgcatgagca
     1681 cgggagcgcc cgtgtattga tgaagtttgc tcaagccgcc gacgtgatcg atgtgcgcgt
     1741 gcgtgctgac gatggctttc acgatgagct tatgacggcc gagaagatcc aagatgcgat
     1801 tgacttcgtc gccggggtcc accaccagcg cttcgcgtgt ggcgggatcg ccaatgatgg
     1861 agcaattgca ttgcaacatc cctacgggga tggtgatgtg gatcaatcct tccgtggcgg
     1921 cattgagcgt aggccgaccg ttgctcatga agtggagtat atcatcggag atgaagggat
     1981 tcgcgagagg cgctgcatgg cgtacgtcgc tctttgatag actaaatgct ggaggatgcg
     2041 cgaacgcgcg gaaatggcta caccaggtct ggctctgaac acgccgcttg cggcgctgca
     2101 ccttgccgca ggcgcgaaaa tgggcgtctg gtttgggtgc gcgctgccgg attactttgg
     2161 cgacgcggcc gccgagtatc gcaatgcgcg cgacacggtg gcgctgatcg acaagaacta
     2221 ccgcgcgtac ctctccttcg ccgggcccga ccgggtgcgc tacttgaacg ccatcctgac
     2281 gaacaatatc aaagacctgc gcgcaggcca cggcatcgtt tcgctgctgc tgaatccgca
     2341 agggcacatt ctcgcggaga tcgagactta tgcatttgcg gaccgtctct tctgcgtgtc
     2401 gcacgtgatg attcgcgagc ggctggtcga atggctcgac aaattcatca tcatggatga
     2461 cgtgacgctg accgatgaga cgccgcgcta cggcacgctg gcgctggaag gtccgaaggc
     2521 cgccgcgatt gtcaaagagg tttccggcgc cgacgtcacg aagtttgagg aactttcctc
     2581 tcacggcgga actgtcggtt cgattccctg ctacatcgtg aagcgatctc cgggaggagt
     2641 tcccggggcg gagttcgttg ccgagagtga aaagctggcg gagctttggc agattctttc
     2701 ggatgcggct cgctggcacg aaggcgggcc gatgggatac gcggcgctga gcgcaactcg
     2761 cctcgctcag ggtgtgccgt ggtttggcta cgacttcggc gaaaaacaga tcccgcacga
     2821 agcgggactg caggacagcc acatcagcta caccaaaggc tgctacacgg ggcaggagat
     2881 cgtggagcgc gtacgctcgc gcgggcaggt aaaccggcgg cgcgtggaac tgctcttctc
     2941 cggcgacgcg gtgccagtgc cggaagcgct cctgaccctg gacggcaaag aagtgggcta
     3001 tgctacgcga gcggcaagga cctgggaccc gccgcggatc atcggaatgg gctacgctcg
     3061 caaagaagcc aacgcgccgg gaagcgttct gcagtgtgct ggcggaaccg cgacggtcac
     3121 gaggtaacga cgaaaagggc ctcgtgactc gtgactggtg acctgtgcta gagaagagta
     3181 aggcctcagc cctggggggc tgaaatacag gcttgaggga aggaactcta gttaacagtt
     3241 catgccccca cccccctctt ttttcggatg tgtggcaaac aaaggacttt aacccctgtg
     3301 tttttggatg tgtggcaact aaaggagtta tgggcgcgtt ttgcggaagt gtggcaaata
     3361 aagaagttag gaggaagtgg ccagtgatca ggggacagtc tcgaccgggt cgggatctcc
     3421 gcttgatgac tgaagtggag cttcgatggt cagagaagaa aaatgctgaa tagggattgc
     3481 cactgaggca gagagtgcac tgagcggaag aagggctatt caatgtgaca gacccctgtc
     3541 cgcgaagcgc cctgattggt ccggggcagg cggagcgtgt gagagaagcc gtgcccaccc
     3601 acccccgttg tgacggcaaa gagtgtggaa ccgtttgatt tgaaatgagt tgttctgggc
     3661 tgttgtccaa ttgaacgagg aaagagtggg gaaaaatatg aaacaaaaga acttgaatgc
     3721 agtggcgagg ggtgtgtagt gaagatggag aggtagcgga caaagcggtg cgatcctttc
     3781 tccgccacgg tgaaaagcta tgagagtata acgcaagagg tactggctgt caaggtttat
     3841 ttagtgtgta attcgaatga tgggggaaga tgttgaaagg atgtggcttg ccaaatcaca
     3901 gagccacaac ccgtaccctg aaaaccgtgg ggtgcggcac gcggcttgaa ccgtccgaag
     3961 tggttcggaa tagagttggt ttcggaacgt gggccagcac gcgggggctt gatgcgcccg
     4021 cctcgcagga gtaggattct gagcaacgct gaatctctca ggaacccccc agcccgtccc
     4081 cgagacacac cgtaaggagg ttctactatg cccaccatga aggagttcac aatccgacta
     4141 gaagaccggc ctgaaacgct tggacaactc tgccaggctc tcgccgatca aagcgtcaac
     4201 attctcgcgt ttcaatcctt ccctatcgag aatggcaaga gctcggtacg tctggtcttg
     4261 gacaatccgg tagcggccaa aaagattctc gatggccgac gcacggatta cacggagacc
     4321 gaagtcgcgc aggtgagact tgccaatcgt cccggcgagc tggcgcgtgc ggcgtcccgg
     4381 ctgggccttg cgggaatcaa catcaactac ggctactgcg gagccgggcc cgacgccaag
     4441 gcaacgttcc tgatcttcgg ggtcgcagag gcaggtaagg cagcgaaggt tcttgatcaa
     4501 ccggccgctg ccggcgcggc ggtctgaacc acggaactgc gactgcacgc tgctcacagc
     4561 ccttacgagt cgcaagaaag gccccgtaac gtaggccgct gaaactcgca gggaatttct
     4621 gtaggctgga acaggggatc gagtttgatg cactccggcg ggaaatctgc gcggggtgtg
     4681 ctgtctctag gagggaccga tgtggaataa gaagagcaca gatgcgatga gcccattgga
     4741 cgcgacagcg gaccgcgaga cggggtggct gggatcgagt ctgcatgtga aaggcgacat
     4801 ctccggcacc gaggatctgc tgatcgacgg ctcggtcgaa ggactaatcc agcttgagga
     4861 gcggaagctg acggtcggaa cgacggcgaa ggtaacggcg gacatcaacg cccgcgacgt
     4921 ggtggtgtac ggctacgtga aggggaacgt gcgcgcgaag ggacggatcg agatcaagaa
     4981 agacggttcg gtgatcggga atttgacgac ggcccagatc atgatcgaag acggcgcgga
     5041 cttcaaagga tcgatcgaaa tcgacaggag cgcggtggag caagccgaca agaacgtctc
     5101 ctcgcgggcg gcggcggctg gcgcggggcc gatttgattg gcatccgtcc tgtcaatgtt
     5161 catttaactg tacattcgaa tgattgtgga aggtgttgaa gggatgggat ttgccgatcc
     5221 agaaattcaa aacccgcatc ctgaaaaccg cagggtgcgg gacccggctt cagtcctgct
     5281 ggacaaaagg cggtggctgg ctgaattctc tccgactttt tcttcacggc ttgggagcgg
     5341 cgtcagcggg cgggatgcgg gaccaaccgg cgggggtgcg gtggaattgg gcggtggcgg
     5401 agagaaggaa ggaaccgcgc tcgtggttcc aggtgaggca ttggatggaa acggaggagc
     5461 gatccacttg aaggatattg aaggaattga gttcgccgcg agcgcgcgag gaggtggcgg
     5521 ttccggcgtg gatgacgagc gcggagtggc cgggaatctt gtagcgcggg gcgctttcgc
     5581 tggtgtggct gatatgcaaa tggccggaga ggattagatc cacgcggcag cgggcaaagc
     5641 cagccatggc catgtgcgcg cggccgacca gaccgtgaag ctcgcgggat tcgggcaaat
     5701 cgaagggatg atgggagatg atgatgcgcg tgcgattttc accacaggct tcgagccggg
     5761 cgcaactgcg ggcgacctgc cggcggttga tgcggccact cttaaaggtg agcgagcggg
     5821 cggtattgat gccgacgata gtgatttcgt catcgaagta gaagggctcg aggtcctggg
     5881 taatgtagcg ctgataatta cgaaggggtt tcagggtgcg agcgagaacg ttgtacaaag
     5941 ggacgtcgtg attgccgggg acgacaattt gcggtgaagg gagagcgtcc agaaaccggc
     6001 gggcttcctg aaattcgcgc tctttggcgc gctgggtaag atcgccggag accacgacga
     6061 cgtcgggccg ggcttcggta acggtggcga gcaaggcctg cacggtggcg ggctcgatgc
     6121 ggccgaagtg caggtccgat agatgagcga gggtgcgcat tttcgttcga gtgcgtcagg
     6181 cgctgttttc cgggacgttt tcgacgggga cgatgacgcg cagaacgccg ggacggatga
     6241 ggtagttcaa cggagtattc atttgatgga cttcgccgtc ggtggaaacg cttgcgtgcg
     6301 gtcggcgcgt ctcgatggag aattgtttgg cagtcagaac atcgagatcg ctggcttcgt
     6361 ttaagcgtcc aaaaaacgca agaaaggcca gccgcaccag gtccatacgg ccggcgcgat
     6421 tggccacaaa gaggcagagc tcgcccgcgt cgagcctttg gcgcccgccg atattcaagc
     6481 cttccatttc atattgatta ttgccgatga agacgaaggg tgtcttgcgc aaaagctccc
     6541 ttccctccaa gcagaggcga acgcggagga gcgagtagtg ccgcagaacc gaaagggagg
     6601 cttgggcgaa agccagccac ttgtggcagc cttgcttctg ttgttcctcg cgccggcgca
     6661 ccatgcgcgg gtagatgccg aggctggaat tgttgaggaa aatgcggccg tttacttccc
     6721 cgacatccag gggtacggca cggccggtga agaggttgcg agctgcggca tcgagatcct
     6781 ggggaatgcg caaatccttt gcgaaatgat tccaagtgcc gaggggaaga acgcccagcg
     6841 ggatggaagt tcccaggagc tcggtggcca cggcgtttag ggtgccgtca ccgccgccgg
     6901 ccacgatgat tttgctattt tcctggacgg cgcggcgagc cagctcggag acttgagcgc
     6961 cgctgggcgc caggaggatg cgggcgggag tgccaatttc agcaaagatt ctggagagtt
     7021 gttcgcggag ttccggaccc gagcaggaac ccgaagcgca attgaggatg caggttaaac
     7081 cggaagaggc cacgtggttt cactgttttc agagcgcgga acgatatcgc cgcgcaatgc
     7141 gttggagcat gggaatcaac tgcagcgaag gcaagtctac cacagtcatg tgaggcctgg
     7201 agcgggggtg aaaggagaag agaggttcct cacctcgccg gccgaccctt tcgcaggagc
     7261 gagaggggaa gaagaaatcg gtctgctcgg ttcggaatga cgggtcgcgg gttagcgcga
     7321 tgggaggagg gagaagagag ggcggactga tccttcgact ccgctcagaa taaaagtccg
     7381 cccctacatg gagcgaagca gatccctcgg gttcgctcgg gatcacagaa aaaagaaaga
     7441 cgcgggccgg gaagccagcg cctattcatt tccctgaaac gcctcgaaat aggtgaatga
     7501 ccagggagat cacggcaaaa aggaggagca ggtggatcag aaaactggtc acatggtaga
     7561 caaggaatcc gcccgcccag agcaaaagga ggaccatgaa caaagccaga aacgcgctga
     7621 ttctcatagc accgtccccc gttcgctgtt ctgcccggta ttacagggtc actctatcat
     7681 cgctgggggg cacgggaaaa tcgaaaccgg aaattggaaa atcgaagaga ggacaacgag
     7741 gagaaagcga cgtcagaggg agtttggggc aaaccagatt tcgtattggc gctggcggat
     7801 gtcgtcgtgg ccggcgatga agttgggctt ggcggccacg tttttcacgc cggaggcaga
     7861 cagttggcca agaatttttt gggcttcgct ctcatcggca gcgtggaagt aacgcgcctg
     7921 ggtctcgtcg gggcccgctt ccaccaggat ttgaataccc gggacaacgt agccattctg
     7981 ccggaagtcg tccgccacca gcctggccgc agggcgctgc gaagccgagc gaatatggat
     8041 gaagatgcgc gggtgaacgc tggcctcggg attggagttt agggagtcct cgatcgtttt
     8101 ctgggcctgc ttaggattcg aggcgtacaa agcggaggcc accgcgctgc cgagtttgtc
     8161 cgcctcggct ttttgtttct ggaggtccgc gatttgattc tggagctgag ccctttgcgt
     8221 attcagtcgc tcaatttcag agtccaacag cgattttcgc tgaaccaggg taggaatgta
     8281 ggaggccaag gcgatagtca cgagcgtggc caggaccgcg aggaaaacct gaatcttgac
     8341 catgaacgga agagaagttt tttcagacga attcatgttt tcacgcgtct ccggcaggtt
     8401 ctggatcttt tacatcgggc ttgagtgcga ctcggatcac catgtacaac actaaggcga
     8461 gccctacaag tcctccgccg accccaagtc ctttgacgct gccgagaaga tcgggcgaga
     8521 tcctgccggt tacgacgcca atcgagacgc tgccgaccag gatcaccatg caagagctgg
     8581 cggagctaac cgccatcacc ttctggatat ccgcgggaga ccatttcttt gccatgacga
     8641 gatctcaaaa aattcatgaa gcggccggac aattctcagc ggtcaacact gaggggaata
     8701 tatcactttc gaagatggcg cgggagtgaa gaagtggggc tcgcacactc gaaaggaaga
     8761 atcaggggcg attcttgcgg accaactgga aggtcatgtg catgatcaac ttcctatcgg
     8821 cttccgagag gagcccgaga atctggcgca actgctggaa gaagcgcgcc gactcacccg
     8881 agctacccca ttcgtcacta ttggcagaga tgccttttgg gagggaccgg aactcgggcg
     8941 gcacttcccc gtcgtaaaac agctggtaca tgggaacttc gagagcacgg gccatctttt
     9001 caagcgtctc gatgctcggg acggtgtggc cgttctcgac acgcgagacg taacagcgct
     9061 tcaggccggt gcgcttctcg atatcacctt gcgaaaggtc tttctgttcc cggatggcgc
     9121 gcaagcgttt tgagataacc atattgttct tagttatatc acgacatcaa tcagtgtata
     9181 cattaatttg aattaaccgc agccggaaca ggactcgcgc gcaggcagcg ttgaataaca
     9241 acgaatgata ctacatgcga actacctagg ctgccggagg atttgcagcg tctgacactg
     9301 acttggggcg aaggagcaag acggcggtga cgcccaagga agcgttctgt gtgaagattt
     9361 cgacgatgta aatgaagcgc tgcgtggttc ccatgagacc gttgggaaca aggagcggcc
     9421 ccgcaccgcc ggtgatccag atgatgctgc ctacagcgac tgccgcgtgc cagcgcttca
     9481 tgcgcaaggt gtagatgata ggaacgaccg cgaaggtcat cagcacccca cgagcgaact
     9541 ggatgaccca gaaccaaaga cccaagcgtg ccaccgcttg agtggcttcc ggatagtagc
     9601 ccttggtgaa aaattgatag gtgatcccac cgaaaatcaa gtagtagaag aggtaggaga
     9661 ggccggagac cagaatcatg aacgctgcgg aaaccgaagt gcgatgctga gagtctggtt
     9721 cgccgggctt gggcattttc agcgacgctg cgagcagagc aagtacggcg gcgacgatca
     9781 ggtacatgac gacggagccg aagaggtcgc ggccggcgtg ctggctgtag ccgggcacga
     9841 agatcagagc ttcagaccag gtgcagagca tggtgacaat cagcagaacg ggaatgatga
     9901 ctccgaattg gccgggagct ttcttcggac cgaagagcgc tacgggaaca aatgatgcgg
     9961 cgaaaagaat tccggagagc caccaccaaa cgaaatggcc ggcgccaaga gcggcgatgg
    10021 tgaccggcaa accaccgagc aagcagtaaa gaacgacgcg tagtttcatc ttcgtgctcc
    10081 ttccttcctt ctttttctgt acgttgcgag agcgcttctg gtccagccaa gcagcgtttc
    10141 ataaccgcgg atgccgcttt caagagcgag ccggccggaa agtgggatgc tcgctccctg
    10201 aactttgaga aactcgcgaa gactggggat ataggcgacc aattcttttg cgagtgcgtt
    10261 caagaaccgc atggcctgga ctgagccagc agtttcgtcc ataaagctga accggagcat
    10321 cagctcatcg acgtgattga tgacgtcatc gctagcgatc gctcttgttt gccaggcttt
    10381 gaaggcggct ctgccagccg cggtcgggcg aaacaagcgg cggcggcgaa gaccggaagt
    10441 tacctgcacg cggcatgcaa cgaaaccgcg tttctctagt ctttgcaaag caggataaat
    10501 tgcgcccggg ctgtcgctga atgatcccat cggggtattc gaaaagatct tcctgaggtc
    10561 gtagcccgag cggggctttt gctggagtaa tcccagaagt gcgaaggcga gcggcgacgt
    10621 gtctttcata gtacgcgaca tactatatcg ctgcgtactg acaaatgtca agcacgagtg
    10681 cgaattgaca cctccactgc gccggctgga tgcacgcgtt aaaatgaaac agtcgagctt
    10741 actcgaattc gcggcgggga gcggattgct cgaggccaat gcgcccggcg gcgatgtcgg
    10801 ccttgaaata gttttcacct ttaatggggg agccggcgag gcggcggagc atggcaatct
    10861 ggccgatgtg attgagggcg tcggcgacgg ggccctggaa gaggccttcc gctgatccgt
    10921 cgagaggctc ggcagaggcg aggaaatcgt caaacttttt tattgccgca aaaaagcgct
    10981 cgatttcggc gctccatggc aatggagtgg aatcgtgcca tgcctgctgg ccttgagcga
    11041 tggagagcgc ccagtcaaac aaatcgccca tgtgcgcgag gatttgcgcg ggcgtgcgcg
    11101 tcttgtctcc aatgtggaag ctggcgaaat ggtccggtgc gccgcggagg gctttgccgg
    11161 cgcggtaggc gacggtggcg acggtgtggc gaagcaactg ccgtgcggga tcggccgatg
    11221 gagtcatgtg gtgattcctc tctactaaga gtggcgagga agagtttaaa cgcagagacg
    11281 cagagtgcgc agagaaaaag agcgaagaga aaagaagcga ggagaaaaga accgaagaaa
    11341 aagtcctcac ccctgaaggg gtgagctaca gggctactgc ttggtgtaga cgcggacgta
    11401 gtcgactagc atttgctggg gaaagacggt ggtgttgtcg ggcgagccgg gccaatcgcc
    11461 gccaacggct acattgagaa tgataaagaa ggggtgatcg aaagtccaag tgccgccagc
    11521 ggaccactgg gattgcgtga aagtgccgta gagattcgca tcgacataga agcggaccgt
    11581 gccgggttcc cactcgacgg cgtagagatg aaaatcgtcg gcgaactttt ggctggcggg
    11641 caggctgaag gccgcggtgg catcgctcgt ttggctaacg gtgctggggc cgtggaggga
    11701 gccgtgtacc gtgccgggct ccttgccgat gttttccatg atgtcgactt cgccgcactt
    11761 gggccagctt acggaactga tgttgttgcc aagcatccag aaagcgggcc acattccctg
    11821 ccctgctggg attttaatgc gggcttcgaa gcggccatag gcttggctga aaaggccctg
    11881 ggttttgagg cgagcggacg tgtaattgcg cgtgacgctg tcggaggggt cggcgtaggt
    11941 ttccttcgcg gcggtgatga cgaggttgcc gccctgaatc tgcgcgttct tggtgcgatt
    12001 ggtgtaagtc tcgagttcgt tgttgcccca cccgttgccg ccaacgccgg tgtcgtaggt
    12061 ccacttggag gcatccggtg aagagccatc ggcgccgttg aactcatcgc tccagacgag
    12121 cgtgaagccg gtggcgggag gaggctggag cgtagcgcct ccgccgcaac tgaacaagag
    12181 cagtgccgcg gcaagaggaa taggaaccag cgcggcagga tgcacgggga atcgtgtttt
    12241 catcgcgatt aatttcgctc gcatgccagc gccaggtccg caccgcgatc aggaaagatt
    12301 caatgcgcgc ttgaaggaac ccttctcatt gtcgattttc ttctggagct gcagaatcgc
    12361 atagagaacg gcttcagggc gcggcgggca gccgggcacg taaacgtcaa ccggaagcac
    12421 ctgattgcag ccctgcacga tggcgtaatt gttgaagacg ccgcccgacg tggcgcaagc
    12481 gcccatggag atgacccatt tgggctcggg catttgatcg tagagctggc ggatcacggg
    12541 agccattttt tgcgagaggc ggccggcgac gatcatgaga tcggcctggc ggggcgaggg
    12601 gcggaacact tcggcgccga accgcgcgag gtcaaagcgc gccgcggcca tggacatcat
    12661 ttcaatagcg cagcaggcca aaccaaagcc gagcggccaa atggagctct tgcgcgccca
    12721 gttcacggct ttgtcgagag ttgtgaagat gatgccttca tcttccagga gattgccgaa
    12781 gtcgtcgggc ctggtcagtg tgacgtgttt gccgagtgat gtgctcatgg tgttccgtcc
    12841 gagctatttt cgcatagggg gcggtcggtg acaaatcgtg ttttggtgac tggcatggct
    12901 acgggttagg gcgctccaag gatttatcca gcaggtctaa ctcgtcttgg agtgccgtgc
    12961 gttttgcctg ctctgtcctt agctccagtt cagcatctgt tgtcttcact tgctgttgtt
    13021 gttctgcttc gtttaaggat tcgagttgca ccctaagcct gggaagcaac tcttcgagat
    13081 ctttccgttc ggcaggattc tgggtgttgc tttgcatatc ctgtcttagt ttgatttcgg
    13141 acgccaggcg cttctgctca gaccgcgtgt gcccaagctc attgtgcgct tcagcaactc
    13201 gccgctctgt gcgcgcgacg gctgcctctt ggatttgcaa acgatagagg agaatctgaa
    13261 ctcgctgcgc ggccacggtt gtggtccgca attcctttcg cagttgacga acctcagcca
    13321 gcaatgcttg cagcgtttgc gagtccgttg aggatgtttg gccgaatgcg atgggcgaag
    13381 acagcacaag acctagtaca agaagccaag agcgatgcat gagaacctcc gggtcaatcg
    13441 taccgccaag actagagact gtcgcctgac ctgagcgtca agatgatagt tggtagtcgg
    13501 acggagagac ggagcgggtg cggcggaggt aggagacgac cgagccgggc cagagagtgg
    13561 tgatttcacc agtgttctgg tcctgatacc aactgttgca gccgccggac tgccaaacgg
    13621 tgccggccat gcggcggtaa atttcttcga caaaggactg ctggatttcc ggacggactt
    13681 ccaggacgcg ctgcttgcgg cgattcatga gcttcaaaca cttcatcgcg tagcggacct
    13741 gggcttcgat catgagaacg acggaattgt ggcccaagcc ggtgttgggg ccgagaagca
    13801 tgaagaaatt ggggaatccg ctgacggtga cgccgagata ggcagacatg cgcgctcccc
    13861 aggcctcgtg aatttccacg ccgcccttcc ccaccacgcg gcatccaatg agcggctcgg
    13921 tggcgcggaa gccggtgcca taaatcagaa cgtcgacggg gcgctctact ccatcgctcg
    13981 tgacgatgct gcgctcgcgc acttcggcga tggcatcggt gacgagttcg acgttcgaac
    14041 gagtgagggt cggatagtag tcgtcggaaa cgagcacgcg tttgcagccc aactgatagt
    14101 tcggagtgag agccgcgcgc atcttgggat ccttgatcct gcgcgcgagg tggcggagag
    14161 caatggcctc gaccttcttg cggaaagtct cgttgccgag aaagccgagg acgcgaatct
    14221 cctggcggca gaaaatatat tgccgcaacg cccagcgcgt gatggggatg ctgcggaaac
    14281 ggcgcctcca tttttcactg aaggcgaaat cgagacgcgg cacaatccaa ggaggtgtgc
    14341 gctgaaagag atggagcttg ccggcgcggg gagcgatttg cgggacgaat tgaatagcgc
    14401 tcgcgccggt gccaactacg gcgacgttct tgccgtccag gtccgcgctg tgatcccagg
    14461 tcgcggaatg aaacgccgga cctttgaaac agtcgatgcc catgagatcc ggataacgag
    14521 gcacgtgcag ggcccccatg ccggagacca gcacgcgggc gcgaatgcgc atcccgtcgc
    14581 ctgccgtggc gttccagact ccttcggcct catcccagat tgcttctcga aagcgtgttt
    14641 tggggcggag gtacggagta agcccatagc gctcgacaca gcgcttcagg taatcgtgaa
    14701 tttcctgctg gcccggatac atgcgagtcc attccgttga gggagcgaag gaaaacgaat
    14761 agagatggga aggaatatcg caggcgcagc cggggtaacg attgtcccac caggtgccgc
    14821 cgatgtcttc gctcttttcg atgatgagaa acgatttcat gccggcttcg aggagcttga
    14881 tgcccatgca aaggccggca aagccggcgc caacgatgag gacgtcaaca ttcgtggcgg
    14941 cggagtttgc ggaaggagtg gcgggttcgg tcatgcgcct gccatcctta cttcttcttt
    15001 gctcgattcc acgggcgcaa cgcgtgtgct gaaaaacgcg gcgctttcac ggagcgaatg
    15061 gcgagcttcg ggaatcaggt gcggggcaag ttgccaggca tgcgggacaa cgggccagat
    15121 tttcagctcg acggggacgc cggcggcgcg ggcacgttcg gcaaggcgcg tggagtcatc
    15181 gcgcaggact tcgtccgcgc ccacgtgaat gagaagcggc ggaaggccgg agaggtcagc
    15241 atagagagga gaggcgagag gattgcgggg atccgtgtca ccaagatagt agcgcgcaga
    15301 tgccgcgacg tctgcgccga tgaacatggc gcaacggtcg gtgttggttc gaatggagtc
    15361 accggtagcg gcaaggtcgg tccagggaga aaagagcgcc gcggccgccg ggagcggaac
    15421 gctggcggcc cgaagagcga gcatgagcga gagtgcaaga ccaccgcctg cggactcacc
    15481 cgcgatgacg atgcgctgcg acggataacc cgtgctcaaa agggcacggt aaacggcaac
    15541 ggcgtcttcg acggcggcgg gaaagcgatt ttctggcgcg aggcgatagt ctggagcaaa
    15601 gacgcggaac ccctggagtg caaagaaaac ggtgatggag cgatgcgact ccgccgaaca
    15661 cccgaagtag ccgccaccgt gcaaataaag cagaacggtg ttgcccggtt tcggaccttc
    15721 cactcgttct ccaggtatgc cgcctagagg agcgggagaa atttggattg atggcggaat
    15781 tttgaaagaa tccggacgaa ggatctggcg tgccacacga acatcgcgca cacccttcaa
    15841 tcggcgctta acgcgccact tgattaccca caccgaaagc cgcgcctgaa gactcgccat
    15901 tgctccctgc tccacatttt cgggatacag aacagggaga ggatatcact cctgtgattc
    15961 acgggggatg atgaaagaag cggagcgctc gccacaaaga aaatggaagg gcaggcgagg
    16021 gagtcaggag gcctcgccgg cccgtgttgc gtcactagag ggaacgtagg aaagtgatga
    16081 gctggttttg ctgcgcgggg ttgagccgat tgaagtattc aatcacctcg gaggcttcgc
    16141 cgcggtgatg ctggatggcg tcaaagaagg tggcggagcg gccatcgtgc agcaactccg
    16201 tgcgcgtgcg tactccccaa agaggaggcg tgcgcaattt gcgagcggtc tcctggccgc
    16261 cggtttgcac gatgccgtcc ccggtgccga cgtcatgcag caggaagtcg ctgtagggat
    16321 ggatgatctt gttgcccaaa gaatcgggca cggtgaacgc tcccgcggct acggccgtgc
    16381 ctgttggcgc cgtagtgatc gaggaaacgt ggcagtgctc acagccgacg ttatggaaaa
    16441 ggcttgcacc agttacgctg tctgaagaag cgcccagcgc ggtgtcgcgg ggcggtgctt
    16501 tggtggcgcg tatgaagcgc gcgaaacggt cgatgtcctc caagccctcg gaatcggggg
    16561 tggcattgtt gggatctgga atcacatcgc aaatcctggt gacgtcgaca ttgttgggag
    16621 gcaaacgatt gctaacgccc atctcgttca aataggcatc gctggagaac gagagaagcg
    16681 aagcatgctg gtccttccac ccgaagcggc cgacggcaag tccgccggtg gactccagaa
    16741 caggaacatg cacggcctca ccgcaaatcg cgccacgcgt gcgcgagcac tgggatgatg
    16801 ccaggtcaag gagagtctgg tctgcgatag attcgatgaa cccgtcgccg agagtgttga
    16861 gagacatgcg gaaagcgcgg atagtttccg cagtgctgat gtgttcctgt ccctgagttt
    16921 gaggaaaact aaatgattgc ggctggccgt tgacggtaat cgtgtccaca gccgggcaga
    16981 cggcgcgctg gttgatgagc gagcggttta caatcgtcac cgtgccatcc gccagagtcg
    17041 ctgttgcgcc gacaaagttc ccgtgggcgt catgatggcc cgcgcggagt tccttgacct
    17101 ggctggttga gccggtgacg gggttttggt ggcactcgcg gcaggattgc gcattgaaga
    17161 tggggccgag tccatcaccg ataaactcgg tctgttcaaa ctgaacgcgg tcagctgcga
    17221 acgatgccga gtcggttagg ccgttgggtt gattgtcgta accggatgga gcttcggtgg
    17281 cagtgctgga attctgcccc gaggatttgc cggccatcgc tagcgccatg gccgagccgg
    17341 tgagtaaaca cacgtatacc gtcgatttct taaaccacat cccgtagtcc tccaagtcga
    17401 gaatcgattg gagattgtta ctgggcaccg ggaggaatca tggttgggag atgccacttc
    17461 cgggaagaat tagcgcggaa aatatcaaac ggccagatgg ttgtatacgg tacttaagga
    17521 tagtcttcga acaggatgga atcgtacagg gtcgagatca gactgtgagg acgactttgc
    17581 cgaactgcga actggattcg agataggcgt gcgcggcggc gacttccgct aacggaaaga
    17641 cgcgatcgac gacgggcttg aggtggccgg cggccacgag tttcatcacc gtgtgcagct
    17701 cggacttggt acccatgtag gaaccgagga gcgaaagctg gcggctgaat aggaacctca
    17761 ggtcgacctt ggcgtcgtag ccggtggtgg cgccgcaggt gactaggcgg cctccggcgg
    17821 cgagactggc gaagctttcg tcccaggtgg ccgtcccgac gtgttcgaag acgacatcga
    17881 caccgcgctt attggtgatg cggcggactt cgtcgcggat tttttgagtt ttgtgattga
    17941 tgagatggcc ggcgccgagg tgctttgctt tttgcagctt ttcatccgtg ccagcggtgg
    18001 cgatgacgcg cgcgccgaag aatttggcga tttgaatggc ggcgctgccg acaccgctac
    18061 cggcgcccag gatcagcaca tcttcaccgg gctgcaattc ggcccgtgcg acgagcatgt
    18121 gccaggcggt ttggaagacg agcgggatcg aggcggcttc ttcgaaagtg agattttccg
    18181 gatacggcag gcaattcact tcaggagcgc ggacgaattc ggcgcagccg ccgtcgatca
    18241 tgtagccgag gttggtgaac tggcggcagc ggttgtcctg gccggagacg caggcggcgc
    18301 atttgccaca ggtcacgccg ggggcgagga cgactttctg gccgacgcgc acagtggaaa
    18361 cgtccgcacc aatctgggcg atctcgcccg cgacgtcact gccgggaatg tgcggaagcg
    18421 gaatcagcac gctggggatg ccgcggcgca cccagagatc aagatggttc agagcgcagg
    18481 ctttaacacg tacgagaaca ccggttgggc cgaaggtggg atcaagcgcg tcctcgtact
    18541 tgagtacgtc gggcgtgccg tggtgatgaa atcggatggc tctcaatcgg atgcaccttt
    18601 gctcggacca ttcgggagag ccattctagc gcgaaaacac gaacatgcgc ttgcgtgaga
    18661 gaaggtcgtg ggtaagctcg gtcctgagct tgctgcagag atcctggaga agggtgtact
    18721 ccgaaggact cagggtctta gggtttggtg cgcgtgttgg atcgttcagc gccttctgca
    18781 aattgccata cgcctttcgg gcggaatcgc tgagatactc accaccagcg aaataatacc
    18841 agtggcgaag ttcttcactt aagttctgga gttgagaata cgtaactccc gcctgctggg
    18901 gccatcgcga caacatggag gttttttgcc ataacccctt gtaagcctcg ccacgctgtt
    18961 tgtacaaagc caggtccaaa cctcgacgat gaatcagaaa gtggctaatc agtgcgccga
    19021 tgaaggcacc aaacgttgtg ataagagcca caccaatggt gctggatgcg gagtgttcca
    19081 tgtcacatct cctttcctga tgctcagaga gatagtgaag gggactgacg tggcgtatca
    19141 cggatccacg cagacggaca gcacgccagt caattgacag tggttcatgc ggagcatagg
    19201 ctgagatgga ggcgagcgtg gacttatttg aagagatcgt gaggatgcgg cgagcggggc
    19261 agcgcgcggc actggctaca atcgtgcata cgaacggctc gatccccagt tacgagagct
    19321 cgcggatgct ggtgcgcgag gatggctcga tcgcgggaac cattggcggc gggtgcgtgg
    19381 aggcggaggt ctgggcggcg gcaaaggaag tgatgcagaa ggaagcgccg cgcaagatgg
    19441 tcttcaacct gaacaatgaa gcgagctacg acaacggttt gatttgcggc gggacgctgg
    19501 aagttttcgt cgagcctatt ttgccacagc cgatgctgta tctctttggg ggcgggcacg
    19561 tctcgatggc ggtagcgaaa gcggctagcg cggcgggctt cggcatcggg gtggtggacg
    19621 accgggaatc ctttgcgaac aaagagcgtt ttccgatggc ccacgagatt tacacaagtt
    19681 acgaggatgc gtttgagaaa attcatccga atgcagcgag ctatgtggtg atcgtaacgc
    19741 gcggacacaa agaagacatg cgggtgctgg cctgggcggt gcgcaccgat gtccgttacc
    19801 tcggaatgat cggaagcaaa cgcaaagttt tgtccgtgta caaggcgctg gagaacgacg
    19861 gctacaagcc ggaagagttc gaacgcgtct acgcgccgat ggggctggag attggcgcgc
    19921 tctcgccgga agaaattgcc gtgagcattg ctgcggaact cgtggcggtg cgccggaatg
    19981 cagcgagcgc cgcgcacaaa aaattgaaat acgaagcgca agcttcgccc attctccaaa
    20041 cgggcgagcg ctgaaaggga gcacatgctc gccgccgtca tcctctccgg cggggcctcg
    20101 agccgaatgg gctcgccgaa agcgctgctt cccttccagg ggcggccctt cctcgaacat
    20161 cttctggaag tgacttcgcg cccggaaatt ggcgcgaggc gggttgtgct cggcgcgcac
    20221 gcggaaccga tcgcgaaggc gataaacctc aaagccgatg aaatcgtaat caatcacgaa
    20281 tgggaaaaag gacagttgag ttcgatccag gcagcgctgc gaagcctgcc gccggggacc
    20341 gacggaatcc tgctctgcct gatcgatcac ccccttatca gcagcgcgct ggttcaggaa
    20401 ttgatcgaac agttttacaa aacgaagagt cccatcgttc tgcctgtcta tgaggggcgc
    20461 cgaggccatc cagtcatttt ctccgcatcc ttgtatgaag aactgcttcg cgcgccgctt
    20521 gaaacgggtg cccgggcggt ggtatgggcg cacaagggag aaatagcaga ggtgcggacg
    20581 aacgaagagg gctgcgtgct gaatctgaac gatccggaga ctatgaatac cgcgctccgg
    20641 caaaaggact aacgatttgc agaaggcagt ttttgaagga gaccgctgac cgcgtcagcg
    20701 gcgagttgtg cgccgtgaaa ggtcgccggt ggaagtccgc cgagagactg agagatagaa
    20761 tcggcggtga gggcgcgggc ttcggcaggc gtgcggccac ggagctcttc cgtcaagatg
    20821 gaggcgcagg cgatggcggt agtgcagccg cggcagagga agcgcgcttc cgcaaaacgg
    20881 ccgtccacga ttcgagcaga gagcttcagg atgtcgccgc agacgggatt gctgacctcc
    20941 accgtggcgg tagcgccggg aagttcgccg gcattgcgcg ggttgcggaa gtggtcgagg
    21001 acggcttcac tgaacatttg tgaaagtatc ccagaggcag tgccagaact gcaacgcggc
    21061 acaaatcaag aaaactcaac gcagagacgc tgagttcgca gagagcagaa ggaacgcgta
    21121 agaacgccct ttctaagaaa agacttactt cttcggtggc aaatactttt tctcgatgga
    21181 ggcaaccacg acatagtttg gatcgaccat ttcggtgaga tggatcttgc caactttgga
    21241 aagtagctcg taggcgtcga gttccgagag accatagtcg gcgtgaatcc accctaccag
    21301 ctcggtgaag gcgattcgca ccgcgtcgtc gacggggcga taaattcctg cggccatgat
    21361 ttctttttcg ttttcgaggc ggggccatcc cgtgtgcttt cctttcacca ggtcgaattg
    21421 cagccgaact ttcatgggaa cttcaatggc gctgcccgca atttccccat cgcccatggc
    21481 ggcgtggcca tcaccgaagt aaaacagggc cccggatacg ttcactggaa gatagaggat
    21541 gtttccaacg gagacttccg gagcgtccat gttcccgccg aattccgcgg gcacgatgga
    21601 actgcgcgct tccccattgg cgggcgcgac accgatgcag ccgagaaatg gatggagcgg
    21661 gattttcacc ttgaagttgg aatcgagcgc ttgaaacgtg gcggtgtggt ccgcgtggtc
    21721 gattgggtaa aaccagattt tttcgggcag cgcttctttt tcgagcatcg gtgtgtagtg
    21781 cgtggcgttg atggcgccga agcccggtga aaacgcgccc acaccctgct tgccatcgac
    21841 ctgcaaatcg aggatatgaa caaccagcgt gtcgccggga tccgcccctt cgatatagaa
    21901 aggaccggtg agcggattat cgcctttgat aagcgcaaag gtatcgccgg gtttttgtag
    21961 cacgttgccg aagcagtcca gagagttcgc gtcgagaatg ttgccgggtt tgagccgcgc
    22021 aacgggcgag gcgacaccga agagatattt caccgtgtcg atggtggcgt ggtactggac
    22081 gacttcggat ttggacggcg cagctggagt ctgagctaga gttgagagcg gcagaatcag
    22141 gctaagagca acgagatgga cacgcacaat aggcctccgg gaattaagaa tcgcagtgca
    22201 acacactcgt ggagcggagt caatcctcac tcgtgcgagc caacggacgc cccgctgtct
    22261 gctatccttt tccagtagcc atgaaagaga atcggcgatc tcctttgcgc attgtctcgc
    22321 tggcccccag cgcgacctcg attctgtgcg ccattggggc gaagcgcgca ctggtcggcg
    22381 ttacgaagtg gtgcgctgac gtcgcgccgg tcggcagact gccgaagctg ggcgattgct
    22441 ggcacttgca atcagtggat gagcttctgc ggctgaagcc gacgttggtg atcggctccg
    22501 tgccgtacaa gaaagagacg gtagcgaaac tcctcgagca gcggctcaat tttctagcca
    22561 tgaacccacg gaccctcgct ggcatcgaag cggacattcg cttgttgggc ggaattacgc
    22621 agcggacggc cgccgccgaa aaggttgtga aaacaatgcg ccgcacattt tcggaactcg
    22681 agaaaaaagc ccggcgatgg aaacagcggg cgcgcgtcta ttgcgaggcg tggccgaatc
    22741 cgcgcatcag ttctccacct tgggtggcgg agttgacgca catctgcgga ggcgagatgg
    22801 tggttcccgc tggcgagaag atcaccgaag agcaggtagc ggcggctagg ccagatgtga
    22861 ttgtgctcgc ctgggcggcg acgggatcga aagccgaccc caagcaggcg tacaaagttg
    22921 cggcgtggaa agatgtgccg gcgattcgaa acagaaacgt ttacgtgatc agtgatgaat
    22981 tgctgaacac gccagggccc ccactaatgg agggtgcgcg cgcactcctc aaactttttc
    23041 attccgtgaa tagaatgcga gaacggtcat gaagactgtc gtcgcggcag tgattgagcg
    23101 gagcgaccgg cgattgttga tcggccagcg ccggaaaaac gatacttccc cgctgaagtg
    23161 ggaatttcct ggtggaaagg tccgcgacgg cgaatcgccg gaggttgcgc tcgcgcggga
    23221 attgcaggaa gaactcggcg tgacgctggt gagaagcgtt gagcttggcc gcgtgcgtca
    23281 tcaatatgcg gaaacgcacg atgacctgga gatccgattc tttgccgcag caatcgcaga
    23341 atcagagctg gcgccgcgca cgtttgaaaa gatcgtctgg gccttgccaa aagagcttgg
    23401 agagtacgat tttctggcag cgaatcgcga actcatcgcg caattggcca ccggacggat
    23461 caagcccgca gaaatcatcg agagtgcaga agaaaccggc gcaagccacg gcccggccgc
    23521 ggactgattc cgtattttct aaatgacggg cgtcaggaga tcaccggatt cggcttttca
    23581 gtgttgatgc ccagatcctt gatggctttg tcgaggacgg agcgctcgat tttgccgtcc
    23641 acgaaaag
//
LOCUS       DASYVO010000095         3901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_200664, whole genome shotgun sequence.
ACCESSION   DASYVO010000095 DASYVO010000000
VERSION     DASYVO010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200664"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1860
                     /locus_tag="VGR03_03085"
     CDS             <1..1860
                     /locus_tag="VGR03_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEV1993294.1"
                     /translation="DYVTLRNDRFVIPVRAENRRSVPGVVHGSSASGQTLFLEPLEAL
                     EVNNQLVQLTEEEASEITRILQDLTRRLQSRLAELRAAAEAIAELDSVFARARFAREF
                     DAAIPEFSEAGALELDSARHPVLEDKLRREGRAVVPITLALGGEDRVLVISGPNTGGK
                     TVALKTTGLAALAAQSGIPVAAQRAVVPMFDRVLVDIGDEQSIAADLSTFSAHMLNLK
                     SMLEAATPRSLVLVDEMGTGTAPEEGAALAVALLEEFRAKRCLVLATTHHDRLKTYAS
                     TTAGVVNAAVEFDEVHLRPTYRLIVGMPGGSSGISIAQRLGLAAEIIERARKLLAPEA
                     LEAADLIAYLHRSRDVLDRMHRQMADERGALEEERKKLRTEWVERQQKRIAELELKFA
                     EMQKRFDENVARVVEAVKEHELRAQMEKTARRKIQDARGEAREDLNAAVVQTLSDSQV
                     DLGITAPVETVSAERLVPGARLLVKGFRQPVVLRRLDGSSAEVEAGPLRMKVAVEEIT
                     GIAGDRAGRKVTQEHKPRGITVHAQPGEGAATDEINVIGLTVEEASQRVDKFLDEAAL
                     ANKPRFRIIHGHGTGALRKGLAKFLSTHPLVERHSFETEDRGGKAITVVELRR"
     gene            1913..2512
                     /locus_tag="VGR03_03090"
     CDS             1913..2512
                     /locus_tag="VGR03_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993295.1"
                     /translation="MFWRNALANFVGSVAAAGFLWFLVTRFYELPRSRKQARELVSMS
                     YALLKFELVFNELYCRELLRVELGRIPAGFPVTQGWETLHSTEAFRYLPPSITGKLIV
                     IYSLLFRLRGNVEFVHRLLLEERRPIDGPNPYSILLVEVSNLTKQVAEKILIAQSAFA
                     QVLQNEIDKLSAEDKKVFDQAYQLHQAETKRNMPKAAAG"
     gene            2630..3313
                     /locus_tag="VGR03_03095"
     CDS             2630..3313
                     /locus_tag="VGR03_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993296.1"
                     /translation="MDKDGAFLAQNWQCGKVIRYLEAGEAQKHLLVCFLQERHYERFF
                     EPIGSLIHAGRTQRGFGFSIMSLCCLLIETIECYCLGWPSSSPKELRQLEASPHNLAV
                     PSNDFKISGPYEPHKYSSEQAFIKFFDDSLHNLHFPDLVGKGRNFYSSIRCGLLHQAQ
                     TKDGWRIRTSGKLYDDSPDKKSINRDAFAQALQNCFDSLLKELEKAEWDGALWKAVRK
                     KMWWLAVTS"
     gene            complement(3587..>3901)
                     /locus_tag="VGR03_03100"
     CDS             complement(3587..>3901)
                     /locus_tag="VGR03_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993297.1"
                     /translation="HHADDVRLLLHLVRECSRFRQLPVPFCCLYLFEFFDIGARKIEV
                     MGATNVQRQILFTRSVVSGRAFQRDRQVCPHSTPLSIPSSYNNSLAAIGRRKALVLVK
                     AF"
BASE COUNT          875 a         1036 c         1217 g          773 t
ORIGIN      
        1 gactacgtca cgctgcgcaa cgaccgcttc gtgattcccg tgcgcgcaga gaaccgccgc
       61 tccgtgccgg gagtggtgca cggctcgagc gcgagcgggc agacgctctt cctggagccg
      121 ctcgaagcgc tcgaggtcaa caaccagctc gtgcagctca ccgaagaaga ggcttccgaa
      181 atcactcgca tcctgcagga cttgacccgc cggctgcaat cgcggctggc ggaactgcgg
      241 gccgcggcgg aagcgatcgc cgagctcgac agcgtattcg cgcgcgcgcg gtttgcgcgg
      301 gagttcgacg cggcaatccc ggagttttcg gaagcgggtg cgctcgagct cgattcggcg
      361 cgccatccgg tgttggaaga caaactgcgc cgcgagggcc gcgccgtggt gcccataacc
      421 ctggcgctcg gcggggaaga tcgcgtgctt gtcatcagcg gcccgaacac gggtgggaag
      481 accgttgcgc tgaagacgac gggcctggcc gcgctcgcgg cgcaatcggg gatccctgtc
      541 gcggcgcagc gcgccgtcgt gccgatgttc gaccgcgtgc tggtggacat cggcgacgag
      601 cagtccattg ccgcggacct ctccacgttt tccgcgcaca tgctcaacct gaaaagtatg
      661 ctcgaggccg ccacgccgcg ctcgctcgtg ctggtggatg aaatgggcac gggcacggcg
      721 ccggaagaag gagcggcgct ggccgtggcc ctgctcgaag aattccgagc gaagagatgc
      781 ctcgtccttg ccacgacgca tcacgaccgg ctgaagacct acgcctccac aacggccggt
      841 gtggtcaacg ctgcggtcga attcgatgaa gtgcatctgc gcccgaccta ccggctcatt
      901 gtcggcatgc ccggcgggtc gagcggcatc tccatcgcgc agcggctggg ccttgcggcg
      961 gagatcatcg agcgcgcacg caagttgctg gcgccggaag ccctcgaggc cgccgacctg
     1021 atcgcatacc tccaccgtag ccgggacgta ctcgaccgca tgcatcggca gatggcggac
     1081 gagcggggtg cgctggagga agagcgaaaa aaactgcgca ccgagtgggt tgagcgccag
     1141 cagaagcgca tcgccgagct cgaactgaag tttgcggaaa tgcagaaacg cttcgacgaa
     1201 aacgtggcgc gcgtggtcga ggcggtgaag gagcacgagc tgcgcgcaca gatggaaaag
     1261 acggcgcggc ggaagatcca ggacgcgcgc ggagaggcgc gcgaagacct gaacgctgca
     1321 gtggtgcaga cgctttcgga ttcgcaggtc gatctgggta taacggcgcc ggtcgaaacg
     1381 gtcagcgcgg agcggctcgt ccccggcgca aggctgctcg tcaaaggttt ccgccagccg
     1441 gtggttttgc ggcggctgga cggctcttca gcagaagtcg aggccgggcc gctgcggatg
     1501 aaagtggcgg tcgaggaaat tacgggcatc gccggcgatc gcgccggtcg caaggtgacg
     1561 caggagcata agccgcgggg cattaccgtc catgcgcagc ccggcgaagg cgctgccact
     1621 gacgaaatca acgtcatcgg tctgaccgtg gaagaggcga gccaacgggt ggacaagttt
     1681 ctcgacgagg ccgctcttgc caataagccg cgtttccgca ttattcatgg acacggcacc
     1741 ggagccctgc gcaaaggcct ggcgaaattt ctttccaccc atccattggt cgagcggcac
     1801 tcgttcgaga ccgaagatcg cggcggaaag gccatcacgg ttgtggaact aaggagatag
     1861 ctatacttat catcagactg atacacattc tgttgaggca aaggagagaa aaatgttttg
     1921 gcggaacgcg ctagcgaact ttgtgggctc cgtcgccgct gcgggtttcc tctggtttct
     1981 cgtaacccgt ttttacgaat tacctcgatc aaggaagcag gcgagagaac ttgtgtcaat
     2041 gtcatacgcc cttttgaagt tcgagctagt tttcaatgag ctctattgtc gagagcttct
     2101 gagggtcgag ttagggcgga ttcccgctgg atttcctgta acgcagggtt gggagacatt
     2161 gcattctacg gaggcatttc gatacttacc gccttcgatc actggaaaac tgattgtgat
     2221 ctactctctc ttgttcaggc ttcggggaaa cgtggagttt gttcacaggt tgctcttgga
     2281 ggaaaggcga ccaatcgatg gaccaaatcc atattccatc ctccttgtag aagtgtccaa
     2341 tcttactaag caggtcgcgg agaaaatact gatcgcccag tccgcgtttg cgcaggtgtt
     2401 gcagaacgaa atcgataagc tcagtgccga agacaaaaag gtctttgacc aggcatatca
     2461 gctacatcaa gcggaaacga aaagaaacat gcccaaggcg gcagctgggt aacatgagat
     2521 ggcggaagcg ggaacattcg cggacgaggt gaagagcagg cggacatcgt ccgcgtcgtg
     2581 gccaggccgt aactttggtt gacctttatc tggactgagg tagtcggata tggacaaaga
     2641 tggtgcgttc ctcgcgcaga attggcaatg cggcaaggtc attcgatacc ttgaggcagg
     2701 ggaggcgcaa aagcacctat tggtgtgctt tcttcaggag aggcactacg agagattctt
     2761 tgagccgata ggaagcctta ttcatgctgg gcgaactcag agaggattcg gcttttccat
     2821 catgagtctg tgttgtttgt tgatagaaac catagaatgc tattgcctcg gttggcctag
     2881 ttcgtcgcca aaagaactgc gtcagttaga agcgtcgccc cataatctag cggtgccgtc
     2941 taacgatttc aagatctcag gtccctatga gccgcacaag tactctagcg agcaagcttt
     3001 tatcaagttt ttcgatgatt cgcttcataa tctgcatttc ccagatttag tcggcaaagg
     3061 gcgaaatttt tactcttcga tccgatgcgg gctactgcat caagctcaga ctaaagatgg
     3121 ttggcgcata cgtacgtccg gaaagctcta tgatgattct ccggataaga agagcattaa
     3181 ccgcgacgca tttgcgcagg cgctacagaa ttgcttcgac tcccttctga aggaactcga
     3241 gaaggccgag tgggatggcg ctctttggaa ggcggtccgg aagaagatgt ggtggctggc
     3301 cgtcacaagc tagggttgct tgcattctga aacggtggat atgaaacctc gtcacaagac
     3361 gcatgccgag aaccaacgcg tatttgcgta tggctcaaac atggatttga gccagatgaa
     3421 agagcggtgt cctgactcca ggctccagcc ctttgtcgcg agattgaaag gttggagact
     3481 ctgctttcca aggtacagtg aaaaacgcaa cgggggcgta agcagcatcg tccgcgatca
     3541 tcaagctgta gtatggggcg tagtctatcg cattacaccc agagacttag aaagccttga
     3601 ccagtacgag ggccttccga cggcctatcg ccgcgagcga attgttgtaa ctgctaggaa
     3661 tggaaagcgg agtggagtgt gggcatactt ggcggtcccg ctggaaagcc cgccccgaaa
     3721 ctacgctcct agtgaagagt atttggcgct gtacattcgt ggcgcccatc acttcaattt
     3781 tccgagcacc tatatcaaaa aactcaaaaa gatagagaca gcaaaaagga acaggtaatt
     3841 ggcggaagcg ggaacattcg cggacaaggt gaagcagcag gcggacatcg tccgcgtggt
     3901 g
//
LOCUS       DASYVO010000096         1676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_130303, whole genome shotgun sequence.
ACCESSION   DASYVO010000096 DASYVO010000000
VERSION     DASYVO010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130303"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VGR03_03105"
     CDS             <1..615
                     /locus_tag="VGR03_03105"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV1993298.1"
                     /translation="VRKTQPLPIECVVRGYVSGSGWKDYRATGKICGIPLPAGLRESD
                     RLPEPIFTPATKAATGHDENISFERAAMLLGKELAEKVRAVSLEIYRRAAAYAEPRGV
                     ILADTKFEFGILYHRARDRAAADDLMWIDEAVTPDSSRFWSAAHYSPGGPQPSFDKQF
                     VRDYLERMRWPKTPPGPELPPEIVAATRAKYREAYRILAGRELD"
     gene            662..886
                     /locus_tag="VGR03_03110"
     CDS             662..886
                     /locus_tag="VGR03_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1993299.1"
                     /translation="MKDQLEGLVNQMVERGILFDEAVGEFEKRFIKRVLDRADGNQSR
                     AAEILGIHRNTLSRKIDEYKLDSNGHRRLR"
     gene            complement(982..>1676)
                     /locus_tag="VGR03_03115"
     CDS             complement(982..>1676)
                     /locus_tag="VGR03_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993300.1"
                     /translation="CLNPPQQMDAFWSMARAVTSKSATQVQAAKVKDYLRKLIVNYQG
                     GNVCDSLTDAELNELLQLASTSAERPESYKLPSSADLDAVRTGPPVMTIASVITDLKA
                     GGDKAKLTWLAACGLEFPEVPGKLIEVVPGTDFILLKIAFVTSDEEFEKATSPNMDVK
                     VVGQPVAARVEKDNVVHFTGTLVGYDPEPAFFLHWEKGKVKEEDIPKEKGAPKKPPVR
                     KPAAKKPGAKPS"
BASE COUNT          340 a          525 c          458 g          353 t
ORIGIN      
        1 gtgcgaaaga cgcagccgct gccgattgaa tgcgtggttc gcggctacgt ctccggctcg
       61 ggttggaagg actatcgggc caccggaaaa atctgtggca tcccgctgcc tgcaggattg
      121 cgtgaatccg accggctgcc agagcccatc tttactcccg caacgaaagc cgccaccggc
      181 cacgatgaaa acatctcatt cgaacgagcc gcaatgctgc tcggcaagga acttgcggag
      241 aaagtccgcg ccgtcagcct ggaaatctac cgtcgcgctg ccgcctatgc cgagccgcgc
      301 ggcgtgatcc tcgccgacac aaaatttgag ttcggcattc tctaccatcg cgcgcgcgat
      361 cgtgcggcgg ccgatgactt gatgtggatc gacgaagccg tcacgcccga ttcttcccgc
      421 ttctggtccg ccgcgcatta cagccccggc ggcccccagc cttctttcga caaacagttt
      481 gtacgcgact atctcgaacg gatgcgatgg ccgaaaaccc cgcccggccc ggaacttcct
      541 cccgagatcg ttgccgccac tcgtgccaag taccgggagg cctatcgcat cctggccggg
      601 cgcgaactgg actagcaaga cgggccctca cacaggaagt tcccgagggg agcgtgccat
      661 cgtgaaagat caattggaag ggctcgtcaa tcaaatggtt gagcggggaa ttctgttcga
      721 cgaggccgtt ggtgaattcg aaaagcgttt catcaagcgt gttctcgacc gcgctgacgg
      781 aaaccagtcc cgcgccgctg agatcctcgg cattcacaga aatacgctga gccgcaagat
      841 cgatgaatat aagctcgatt cgaacggtca ccgccgcctg cgctagtctg cagctggctc
      901 cttacgaaat gacagaaaga aaaatggctc tccggcaaag ccggagagcc attttgttca
      961 atccaccgga atcgaagatt ctcagctagg ttttgctccc ggcttcttcg ctgctggctt
     1021 gcgcaccggc ggcttcttgg gcgcgccctt ttccttcggg atgtcctctt ccttcacttt
     1081 gcccttctcc caatgcagga agaacgccgg ctcaggatcg tatcccacca gcgtgcccgt
     1141 aaagtggaca acgttgtcct tctcgacgcg cgccgccacc ggctggccga caacctttac
     1201 gtccatgttg ggcgaagtcg ctttttcaaa ctcttcatcg ctcgtcacga aggcaatctt
     1261 aagtaaaatg aaatccgtac cgggcacgac ttcgatcagc ttcccaggca cttcgggaaa
     1321 ctccagaccg caggccgcca gccaggttag tttggcttta tcgccgccag ctttcaggtc
     1381 agttatcacc gacgcaatgg tcatcacggg cggcccggtg cgaacggcat caagatctgc
     1441 gctgctcggc aatttgtagc tttcgggccg ctccgccgaa gtgctggcga gctgcaggag
     1501 ttcattcagt tccgcatcgg tcaggctgtc gcagacgttt ccgccctgat aattcacgat
     1561 cagcttccga aggtaatcct tcactttggc ggcttgcacc tgagtcgcgc tcttggacgt
     1621 cactgctcgc gccatggacc agaacgcatc catctgttgt ggaggattca ggcaca
//
LOCUS       DASYVO010000097         5325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_102112, whole genome shotgun sequence.
ACCESSION   DASYVO010000097 DASYVO010000000
VERSION     DASYVO010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102112"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="VGR03_03120"
     CDS             <1..1373
                     /locus_tag="VGR03_03120"
                     /inference="COORDINATES: protein motif:HMM:NF008868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3,4-dehydroadipyl-CoA semialdehyde
                     dehydrogenase"
                     /protein_id="HEV1993301.1"
                     /translation="IADALTANRDEYFRISLLNSGTTQADASFDVDGAIYTMKYYAKI
                     GRALADGKMLKEGAEIPLSKTGAFAGQHFLTPAKGAAVFINAFNFPAWGFCEKGAPAL
                     LSGVPIVVKPASPTAWLTHRMVEDIVKAGILPPGAISILCGSARDLLDHVSEGDIVSF
                     TGSADTATRIRSHANVLRRSVRINIEADSINSAILGPDGAPGTDIFDLLAKEVVREMT
                     LKAGQKCTAIRRVLVPRQHFKAAGDAISARLSSIKVGNPRNTEVKVGPVVNKAQQSAC
                     LEGLRQLKQECSVLSGGDEGFQPVDADAQKSAFVQPTLLSCENGRDAKFVHDVEVFGP
                     AATLVAYDQLEDLVAIARRGLGSLVVSIFSNDASFIQNVVLGIGDLHGRLLVVDSTVG
                     TQHTGHGNVVPSCLHGGPGRAGGGEELAGLRALLLYHRRFVVQGPPRFIAELAGLSAD
                     ASLLYS"
     gene            complement(1528..3144)
                     /gene="mdlC"
                     /locus_tag="VGR03_03125"
     CDS             complement(1528..3144)
                     /gene="mdlC"
                     /locus_tag="VGR03_03125"
                     /EC_number="4.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF005485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoylformate decarboxylase"
                     /protein_id="HEV1993302.1"
                     /translation="MATVREVTYQVLRDLGMTVIFGNPGSTELPFLRDMPADFRYVLG
                     LHERSAAGMGLGYALARRKAAFVNLHSVASAGNGLSALIDSFYCHAPLVVTTGQQDRR
                     QILTEPFLVSRAVEVVKPYVKWASEPLRAEDVPATIARGYYLAMQPPMGPVFISIPMD
                     DWNHECQPVAVREVTQTVLPDPAALDTVVRALDSSRSPAIVAGSQIEEDRGWHEVIAL
                     AEHLDADVYQEPIATRWTFPRTHRLFRGSLLPAQQPLADQLAGHDAVVVLGAPIFLYY
                     AYVPGNPIQPGTQLFQITNSPLDASAALAGASIVGNLAAAAQYLRTHTKQRKRGAAAP
                     RVKPPEPQPEYPITPSYLFSVLDRVMPRDAVVCEECPSSKGDLERYLQLDQPDSFYSV
                     RTGILGFGLPAAVGLQLAHPDRRVVCPVGDGSIQYSIQALWSAVQYNAAVIFIVLRNA
                     DYSALIAFCDFTQVGRNVPGMDIPGIDMVKIAQGYGMTAQEVDRPEDLEPRLREAFAA
                     QGPRLISVNVAKGWKTCMGMDQSVNPPNYR"
     gene            3460..3954
                     /locus_tag="VGR03_03130"
     CDS             3460..3954
                     /locus_tag="VGR03_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HEV1993303.1"
                     /translation="MANVDTKSSLDAVVREYQFPDIHIGAGEDESPWVPFRENVFIRH
                     LAFDVRSNMYANLLWLKKGGLVGRHRHRGRVFACTLEGSWRYLEYDWVARPGSYVQES
                     PGTIHTLFSDDGMKTVFLLNGSIEFYDDNDTLLETLDVFWFIDHYLSHCRKHGLPVNQ
                     KLFV"
     gene            complement(4043..5191)
                     /locus_tag="VGR03_03135"
     CDS             complement(4043..5191)
                     /locus_tag="VGR03_03135"
                     /inference="COORDINATES: protein motif:HMM:NF015012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-dopachrome tautomerase-related protein"
                     /protein_id="HEV1993304.1"
                     /translation="MKSISIVILFYFSSVSCFGQELAQERVIGKIEVVATFDGPMPTG
                     VTVTNNGRIFVNFPRWGDHVEYTVAEVKDGKTRPYPNADLNHSSDTDNPADKLVSVQS
                     VVVDPGGSRLWILDTGSVGFGPVKPGGPKLIAVDLATNRVIKKIIFPADVALTASYVN
                     DVRFDLRRGAEGMAFITDSTSAGPNGIIVADLASGKSWRRLNDHPSTRRDPEFVPVVE
                     GEILQLRLPGKPPMKFAVGSDGIAISPDGKTLYYCPLTSRHLYSVSVDALADRNKSDA
                     DVAATIKDLGEKGGASDGLESDAQGRVYLSDYEHNAIRRRTAGNEIETLMHDPRALWP
                     DSLSLAADGYLYFTANQVERSPSFHNGQDLRQKPYVLFRMKVDGTRIQ"
BASE COUNT         1053 a         1571 c         1607 g         1094 t
ORIGIN      
        1 aaatcgccga cgcactgacc gccaatcgtg acgaatactt tcgtatttct ctcctcaact
       61 ccggcaccac acaggccgat gcctcgtttg acgtcgacgg cgccatatac accatgaagt
      121 actacgccaa gatcggccgc gcgctggccg atggaaaaat gttgaaggaa ggtgcggaga
      181 ttccactctc gaagaccggc gcgtttgccg ggcagcattt tctgacgccg gccaagggcg
      241 ccgcagtttt catcaatgcg ttcaacttcc cggcctgggg attctgcgaa aagggcgcgc
      301 ctgcgttgct ctcgggcgtt cccatcgtgg tcaagcccgc ctcgcccacg gcctggctga
      361 cacaccggat ggtggaagac attgtgaagg cgggaatttt gccgcccggg gccatctcca
      421 tcctttgcgg ttctgcccgc gatcttctgg accacgtcag cgaaggcgac atcgtttcct
      481 tcaccggctc cgccgatacg gccacgcgga ttcgctcgca cgcgaacgtc ttgcggcgat
      541 cggttcgcat caacatcgag gccgacagca tcaactccgc catactcgga ccggacggcg
      601 ctccggggac ggacatcttc gacctcctgg ccaaggaagt ggtacgcgag atgacgctca
      661 aggccggcca gaaatgcacg gcgatccgcc gcgtgctggt tccgcgccag catttcaagg
      721 cggcgggcga tgccatctcc gcgcgtttga gttccattaa agtcggcaat cctcgcaaca
      781 cggaagtaaa ggttggaccc gtggtgaaca aggcgcagca atccgcctgc ctcgaagggc
      841 tgcggcaatt gaaacaagaa tgctcggttc tatctggcgg cgacgaagga ttccagccgg
      901 tcgatgccga tgcgcaaaag tccgccttcg tgcagccgac gcttctctcc tgcgaaaatg
      961 gacgcgatgc aaagtttgtt cacgatgtcg aggtgttcgg cccggcggcg acgctggtgg
     1021 cttacgacca actggaagat ctcgtggcca tcgcgcgccg cggtctgggc tcgctcgtgg
     1081 tctccatttt ttcgaatgat gccagcttta ttcagaatgt cgtcctcgga atcggcgacc
     1141 tgcatggacg gttgctggtg gttgactcca cggtcggcac gcagcacacc ggtcatggaa
     1201 acgtggtgcc cagttgtttg cacggcggac cgggaagggc gggaggcggc gaggagctcg
     1261 ccggcttgcg cgccctgctt ctctatcatc gccgtttcgt ggtgcaaggc cccccccgtt
     1321 tcatcgccga gttggccggg ctgtccgccg acgcgagcct gctgtactca taaagcttcc
     1381 cgactgttct tcgtcaaagc gaggaccgcg ctccctggag gacgctgcaa cactttctcg
     1441 cgatcttgtt ttctacgcac ttggttcaga tcgtcaaagc catgttgttg tccgggtgta
     1501 ccgctcgctg aaattgcgcg ccggctctca gcgatagttt ggcggattga cggactgatc
     1561 catccccatg caggttttcc agcccttggc gacattgacg ctgatcaggc gagggccttg
     1621 cgccgcgaag gcctctcgga ggcggggctc gagatcttcg ggccggtcca cctcctgcgc
     1681 ggtcatgccg taaccctgcg caatcttcac catgtcgatg ccgggaatgt ccataccggg
     1741 gacgttgcgg ccaacctgcg tgaaatcgca gaaggctatc aacgcggagt agtcggcatt
     1801 gcgcagaacg atgaagatca ccgccgcgtt gtattgaacc gcgctccaca gcgcctggat
     1861 ggaatactgg atggaaccgt cgccgacggg gcagacgact ctccgatcgg gatgcgcgag
     1921 ctgcagaccg acggcggcag gcaaaccgaa tcccaggatg ccggtgcgca cggagtaaaa
     1981 cgagtccggt tgatccaact ggagataccg ctcgagatcc cccttcgaag acggacattc
     2041 ttcgcagacc accgcatcgc gcggcatgac ccggtcgaga acactgaaca aataacttgg
     2101 cgtgatggga tattcgggtt gcggctccgg cggcttcacg cgcggcgcag cagcgccgcg
     2161 tttcctctgc ttagtgtggg tacgaaggta ttgagctgcg gcggcaaggt tcccgacgat
     2221 gctggctccg gccagcgcgg cagaggcgtc gagcggcgag ttggtgattt ggaaaagctg
     2281 ggttcccggc tggatcggat tgccgggcac gtaggcgtaa tacaggaaaa ttggcgcgcc
     2341 caggaccacc accgcgtcat gaccggccag ttgatccgcg agcggctgct gcgcgggcaa
     2401 gaggctccct cgaaacagcc ggtgcgttcg cggaaatgtc cagcgggtcg cgatgggctc
     2461 ctgatacacg tctgcatcga ggtgctcggc gagggcgatc acctcatgcc atccgcggtc
     2521 ttcctcaatc tgagaaccgg cgacgatggc ggggctgcgg ctcgaatcga gcgcgcgaac
     2581 cacggtgtcg agggccgcgg gatccggcaa caccgtctgc gtcacttcgc ggacagccac
     2641 cggctggcat tcgtgattcc agtcgtccat gggaatggaa atgaataccg gtcccatggg
     2701 tggctgcatc gccaggtagt atccccgcgc aatggtcgcc ggcacgtctt ctgcgcggag
     2761 cggctcgcta gcccatttca cgtatggctt gaccacttcc accgcgcggc ttacgagaaa
     2821 aggctctgtc aggatttggc ggcgatcctg ttggccggta gttaccacca gcggcgcgtg
     2881 gcaatagaac gaatcgatga gcgcggagag cccattaccg gcgctggcca ccgaatgcag
     2941 gttcacgaag gcggccttcc tccgggccag cgcgtatcca agccccatgc ccgctgccga
     3001 gcgctcatgc agccccagga cataccggaa atcagcgggc atgtcgcgca gaaaaggcaa
     3061 ttccgtcgat ccgggattgc caaagatcac ggtcatcccc aaatcgcgca agacctgata
     3121 tgtgacttct cttaccgtgg ccacggtcat gctccggact tgagaaatct ccagccgcga
     3181 ggtgcgggag ttataccact tttccaaatg cactcgcgcg gttgaattcg ctttccgggg
     3241 agaaatactt ggcgtcccgg taccgctcaa tcgcgccgtc tggcaacttg taaaggggct
     3301 ggaacaatcc tggaccgatc cgtcgtgaac tctccggcga tcgcggtgga gttcttcccc
     3361 cgcggacgct gtaaatcggg gcgaacttgt gatattttcc ggcgtagtca ttggaattca
     3421 ggatggacct ttcgcaggtg agggcaggcc ggaggattta tggcgaatgt tgatacgaaa
     3481 tccagtctcg atgcggttgt ccgcgagtat cagtttccgg acatccacat cggcgcagga
     3541 gaagacgaat ctccctgggt gccgttccgg gaaaacgtct tcatccgcca tctggcgttc
     3601 gacgtacgaa gcaacatgta tgcgaaccta ttgtggctga agaagggcgg cctggtgggc
     3661 cggcatcgcc atcgtggacg ggtttttgcc tgcacgctgg aaggcagttg gcgatatctg
     3721 gaatacgact gggtcgcccg gcccggcagc tatgtgcagg aaagccccgg caccatccac
     3781 acgctgttct ccgatgacgg catgaagacg gtcttcttgc tcaacgggtc aatcgagttc
     3841 tatgacgata acgacacgct gctggagact ttggacgtgt tctggtttat cgatcactat
     3901 ctgagccatt gccggaagca tggcctcccg gtcaatcaga aactcttcgt gtgaagcgtt
     3961 ccgggtccga gcggaacgta atccatatct cctcgatcct gaaattccac tccgggaagt
     4021 ttccgggata cgcagcctat tgctattgaa ttcttgttcc atccaccttc atgcggaaga
     4081 gcacataggg tttttggcgc aggtcctggc cgttgtggaa cgagggagac ctttccacct
     4141 ggttcgcggt gaaatagaga tatccgtccg ccgccaggga caaactgtcc ggccataggg
     4201 cgcgcgggtc gtgcatcaac gtctcgatct cgtttcccgc ggtccgccgg cgaatggcgt
     4261 tgtgttcgta gtcgctgaga tacacgcggc cctgagcatc ggattcgagg ccgtcgctcg
     4321 cgccgccctt ttcgcctaaa tctttgatgg tggccgcgac atccgcatca gatttgttcc
     4381 ggtctgccag agcgtccacg ctcacactgt agagatgccg gctggtcagc ggacagtaat
     4441 aaagggtctt gccgtccgga ctgatggcaa ttccatcgga gccaacagca aacttcatgg
     4501 gcggttttcc cgggagccga agctgcagaa tttccccttc gaccacgggg acaaactccg
     4561 ggtcacgtct tgtagaggga tgatcgttca ggcggcgcca actcttcccg gaggcgaggt
     4621 cggccacgat gatgccgttc ggccccgccg aagtggaatc ggtgatgaac gccatgccct
     4681 ccgcgcctcg gcgcaaatca aaccttacgt cattcacata gcttgcagtg agggccacat
     4741 ccgcagggaa aatgattttc ttgatgaccc gattggtggc gaggtccaca gcgatcagtt
     4801 ttggaccgcc cggcttgacc ggcccgaacc cgacgctgcc ggtatcaaga atccacagcc
     4861 tgcttccacc ggggtcaacg acaacgcttt gaaccgagac cagcttatcc gccggattgt
     4921 cggtgtcact ggaatgattc aggtctgcgt tcgggtaggg ccgtgtcttg ccgtctttga
     4981 cctccgccac cgtgtattcg acgtggtcac cccatcgggg aaaattcaca aagatccggc
     5041 cgttgttggt gacggtaacg ccggtgggca tggggccatc gaaagttgcc accacttcga
     5101 ttttgccaat gacgcgctcc tgcgcgagtt cctgaccgaa gcacgatacg gaagaaaaat
     5161 agaaaagaat cacaatagaa attgatttca ttgttttcgt tttccttcaa agctcaggcg
     5221 ggttcttgat ggcagggaaa ccgggcccgc gaacagagcg ctccatcgca tatctggaaa
     5281 gtacgggtgc aacggaacga tcgcgcgcgg gtgagactca agcaa
//
LOCUS       DASYVO010000098         6832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_130350, whole genome shotgun sequence.
ACCESSION   DASYVO010000098 DASYVO010000000
VERSION     DASYVO010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6832
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130350"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VGR03_03140"
     CDS             <1..340
                     /locus_tag="VGR03_03140"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1993305.1"
                     /translation="VRELENCIARAVTLGDGKIIDVADLPPAIRSEQADSGIVTSQDT
                     TTMSTTALAEMERMTILRVFEQANGDKALAGKMLGISRATLYRKLKRYNIALKAGSTG
                     GGADPQAAPQ"
     gene            585..959
                     /locus_tag="VGR03_03145"
     CDS             585..959
                     /locus_tag="VGR03_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993306.1"
                     /translation="MLTGRARGDVGNSGGLTLLANEPDCAEQLARFFPQARPVRIPVQ
                     VTVSRGAKTSLREATVIEFGGPEHAIFLSTLPLEFDDRVRLVATGEGQPTEATVVGVQ
                     YHEGRKAVAVRFLQGPCDWVVT"
     gene            962..2446
                     /locus_tag="VGR03_03150"
     CDS             962..2446
                     /locus_tag="VGR03_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993307.1"
                     /translation="MNKMITTLNIPASARSESPRQAQWKEARSVFPIYLALAKQLEIE
                     IPFTPAKRNLPEKPDLETFSQVHEWLDSMDQRVMVHQLRHLLQMTTLNASETGLRALI
                     QRHLRKSKKSNVDRDKIDFLLVQYFALCAPAKIYHKQIEPADVAHVMQPVLGDVDPAP
                     LAWCAPLEEMIEALRGFRSLREILKTNFIEQGRKVKETAGGMFYDPAALVAFIRFNFL
                     LRRTLIELMHADLIAIRAGLGQLQSAGARIIDCHHVGLSATEPLTKICAMADEWKQPF
                     QREYTERSVSQAFDKLLGLRTDVEQALEKALGKSGESAPQPAAAQAGKPLPAAKKSAA
                     ESTASNANAKAKERSTTPAAAAPDTIEVLDFENCMEHIWEQLIATPPSRGRSMTTVKV
                     GGARILMSSWEVTAFVSEDGPSAEDLRRAVVARVLVTAAMESAKETGNATSLDRAMKM
                     ARVEVTRLQERVDVAKQAKDTEAAVNLGISTKRLLSTLDEAEKL"
     gene            2503..3186
                     /locus_tag="VGR03_03155"
     CDS             2503..3186
                     /locus_tag="VGR03_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993308.1"
                     /translation="MRHANVVILTDETEFARLLTACWQTERQAPNIAVLNSDSWGEQD
                     APAHDLVVVGPVRDGKITSVLRSLDPATAVILCVPADSQEFGQLRTKYPRLVHIPLRE
                     DWAQTLLLVAGESLRRAKALQIARQAERNAARNENHATLGRYMLDMKHSVNNALTSML
                     GNAELLLLEPGQLSSQSLAQIKTMHSMALRINEVMQRFSSLASEMQEAENASQAETEE
                     ATAPTSRRR"
     gene            3257..3682
                     /locus_tag="VGR03_03160"
     CDS             3257..3682
                     /locus_tag="VGR03_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV1993309.1"
                     /translation="MQKLAADVHLCSVDKYGESAAVHKKVLIVDDSPAEVKLMQGLLE
                     KEGYWPIGLNDPKRVEETIANERPSVILLDVVMPDRNGFQICRDLKGNAEFNMIPVIM
                     VTSKDSASDKFWGEQQGADGYVTKPFTREDLLRAVRRFA"
     gene            3708..4235
                     /locus_tag="VGR03_03165"
     CDS             3708..4235
                     /locus_tag="VGR03_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEV1993310.1"
                     /translation="MSEDFHLEPLPADADTGDLLEALGPIEERPPESQYCVFRSGRER
                     FCLPVLDVEEVLDWPLLTKLPLGPAYLVGIFNLRGAIVPLIDIAITEGRRPGLLPKHV
                     VVASRRSESHHEDLRIGIASDEVIGTYSVNTEDLLEQAPENVPHCIGMLRHDDRLALV
                     IDLRRLLEVFPGPSI"
     gene            4297..5727
                     /locus_tag="VGR03_03170"
     CDS             4297..5727
                     /locus_tag="VGR03_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEV1993311.1"
                     /translation="MKSRLSSTIWMLVLLNAVVGCFVLGLAFSAGKKAGDASFFDFGS
                     PAGGTILFAIVLALVTWGILAWRFGGLLAPVQALAEFSERLAAGDPRARVEVNSNDEL
                     GYIAENLNRAVARVSKATSNQDANDALQRSITELLSVINQVARGDLAQRGKVTNDALG
                     NVTDSINYMLDNFTKVLERVRKAAMEVTACSNNILVAADEMQAGATQQDQEITNTSSA
                     VEELTVSMKQVSNNAEASAEAARRALDAAEQGNRAVRDTLEGMQRIRASVQATAKKIK
                     SLGDRSLEISEIINVINDITEQTNLLALNAAIEAARAGEAGRGFAVVADEVRKLAEHS
                     RSATKDIAALIKAIQAETNEAVVVMEEGTKEVESGATLADQAGRALDAISNVVRQSAE
                     LVQEISLASKQQVRGTEGVAHAMQIISSITRQTSQGTRGTVATVSQLVKLSDQLNEAL
                     AQFRASSKAAAAHEDSERPVPAGALR"
     gene            5791..6675
                     /locus_tag="VGR03_03175"
     CDS             5791..6675
                     /locus_tag="VGR03_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEV1993312.1"
                     /translation="MSERQGQVQGLSEHELSEIRMLIEERTGICFDESRERFFSTRVR
                     EHLHAKGMERGTELLRAMRKSNVEYEGLLERLLTQETSFFRYPGVYEAFEKRVLPELH
                     TKKFWKNPRTLRIWSAGCSTGEEPYSIGITIADSLSFADAWNVEILATDVGRLALKQA
                     ERGIYKGRSIASVSERQLANHFSAVSGGQQVKPRLRKLVSFAQMNLASAVYVGRMDMI
                     FCMNVLIYFSEERRRSLVQRFYDTLEPGGYLFLGHSESISKMPVKFQAIVLNDCILYR
                     KPTADELQKPELVTEGRA"
BASE COUNT         1563 a         1894 c         2135 g         1240 t
ORIGIN      
        1 cgtccgcgaa ctggagaatt gcatcgcgcg ggcagtgacg ctgggtgacg ggaaaatcat
       61 cgacgttgcc gatctgccgc ccgccattcg gagcgagcag gcagattcgg gaatcgtaac
      121 gtcccaggac accacgacga tgtccaccac ggctttggcg gagatggaac gaatgaccat
      181 tctgcgtgtg ttcgaacagg cgaacggcga caaggcgctg gcgggaaaga tgctagggat
      241 cagccgcgcg acgctgtatc gaaagctaaa gcgttacaac attgccttaa aggcgggttc
      301 gacaggtggc ggagctgacc cgcaagcagc gccccagtag caagagaaac gtctcaagcc
      361 aaatattccg ggaacccagg atagcgcacg cagtgagacg aaaacgtttc acggtgagac
      421 gggtgagcca acgaaacctc ccgagaaaat cttaagcctc accatttctg caacttgcaa
      481 cagccccgcg tttctaattc tttggcgtcc agcgtgcatc tctgtcctcg gaagtctacg
      541 cctcttgcgg tgcgtagtgt gtctcacctc gagagagcgc gtggatgttg accggtaggg
      601 cgagaggcga cgtgggaaat tccggcggtc tgacactcct agcgaatgag ccagactgcg
      661 ccgagcagtt ggcacgattc tttccgcagg ccagacctgt tcgaattccg gtgcaagtga
      721 ccgtgtcgcg cggagccaaa accagtcttc gcgaggccac ggttatagaa tttggcggcc
      781 cggagcatgc tatttttctt tcgaccttgc cgctggagtt tgacgatcgc gtgcggcttg
      841 tagcaactgg ggaaggacag ccgacggagg caaccgtcgt cggcgtgcag taccacgaag
      901 gacgcaaagc agtggccgtc cgattcttgc aaggcccctg cgactgggtg gtgacatagc
      961 cgtgaacaag atgataacaa cgctgaatat ccctgcttca gctcgatcgg aaagcccgcg
     1021 acaagcgcaa tggaaagaag cgcgctcggt gttcccgatc tatctggcgc tcgcgaagca
     1081 actggaaatc gaaatcccct ttacgccagc gaagcggaac ctccccgaga aaccagatct
     1141 ggagaccttc agccaagtgc acgagtggct ggattcgatg gaccagcgcg tcatggtgca
     1201 tcaactccgt cacctcctgc aaatgaccac gctgaacgcc agcgagacgg gactgcgcgc
     1261 actgattcag cgtcatctgc gcaagtcgaa gaaaagcaat gtcgaccgag acaagatcga
     1321 cttcctgctg gtgcagtact ttgcgctctg tgcgccggcg aagatttatc acaagcagat
     1381 cgagcctgcg gatgtcgcgc acgtaatgca gccggtgctc ggagatgtgg atccggcccc
     1441 gctcgcatgg tgcgcgccac tggaagaaat gatcgaagcg ctgcgaggct tccgcagcct
     1501 gcgggagata ctgaagacga atttcatcga acaaggccgg aaggtaaagg aaaccgccgg
     1561 cgggatgttc tacgatcccg cggcactggt cgctttcatc cggtttaatt tcctgctccg
     1621 ccggacacta attgagctga tgcatgcgga cttgattgcc atccgcgccg gacttggcca
     1681 gttgcaatcc gccggtgcac gcatcatcga ctgccaccat gtcggactct cggcgactga
     1741 accactcaca aagatttgtg cgatggccga cgagtggaaa caacccttcc agagggagta
     1801 cacagaacgg tcggtcagcc aggctttcga caagttactc gggctgcgca cggacgtgga
     1861 gcaagcgctc gaaaaggcac tcggaaaatc gggcgaaagc gcccctcaac cggcagcagc
     1921 acaagcagga aagcctctcc cggcggcgaa gaaatccgcc gcggaatcca cggcttccaa
     1981 tgccaacgcg aaagcgaagg agcgttccac gacgccagct gcagcggcgc cggatacgat
     2041 tgaagtgctg gattttgaga actgcatgga gcatatctgg gagcaactga tcgccacacc
     2101 gccttcgcgt ggaaggtcga tgaccaccgt taaggtcggc ggagcgcgaa ttctgatgtc
     2161 gtcatgggaa gtaacggcgt ttgtgagcga agacgggccg tccgcggaag atttgcggcg
     2221 cgccgttgtc gcccgggtgc tggtcaccgc ggcaatggag tccgccaagg aaacggggaa
     2281 tgccacgtcg ctcgaccgag cgatgaaaat ggcccgcgtg gaagtcactc gattgcagga
     2341 gcgcgtggac gtggccaagc aagcgaagga tacggaagca gcggtaaatc tcgggatcag
     2401 cacgaaacgt ctgctctcga cgcttgacga agcagaaaaa ctgtagccga ttgcgagctg
     2461 gtccggcgag gccggacaga aacttcaggg agcgggtttt cattgcgaca cgccaacgta
     2521 gtcatcctga ccgatgaaac cgaatttgcg cggctattga ccgcctgctg gcagaccgaa
     2581 cgccaggccc cgaacattgc tgttctcaat agcgattcgt ggggtgaaca agatgcaccg
     2641 gctcacgacc tggtggtggt ggggccagtg cgcgatggaa aaattaccag cgtgctgcgg
     2701 tccctcgatc ccgcaaccgc ggtcattctt tgtgttccgg cggattccca ggaattcggg
     2761 cagttgcgca ccaagtatcc aaggctggtg catatcccgc ttcgcgaaga ctgggcgcaa
     2821 actctcttgc tcgttgcagg cgaatctctg cgccgagcga aagccttgca gatcgcacgg
     2881 caggcggaaa ggaacgccgc caggaatgag aaccacgcca ctctggggcg gtacatgctg
     2941 gacatgaagc acagcgtgaa caatgcgctg acctccatgc tgggaaatgc cgaattgctg
     3001 ctgctggagc ccgggcaact ttcctcgcag tcgttggcgc agatcaaaac catgcatagc
     3061 atggcgctgc gaatcaacga agtgatgcag cgtttctcat cgctcgcgag cgagatgcag
     3121 gaagctgaga atgcatctca agctgagaca gaagaggcga cagcgccaac ttcgcgccgc
     3181 cgctgaggtc ctccccagcc gctgtaacct actcatttat aaagatttag cctgaaacga
     3241 cacaggggca cgcggaatgc aaaagctggc tgctgacgtg cacctctgct ctgtggacaa
     3301 gtacggagag agcgcggctg tgcacaaaaa agttctgatc gttgatgact cgcccgctga
     3361 ggtcaagctg atgcaaggct tgctggaaaa ggaaggctat tggcccattg ggctgaacga
     3421 ccccaagcgc gtcgaggaaa ccattgcgaa cgagcggccc agcgtgatcc tgcttgacgt
     3481 ggtgatgccg gaccgcaacg gatttcagat ttgccgcgac ctgaagggga acgccgagtt
     3541 caacatgatt cccgtaatta tggtgacttc gaaggactcg gccagcgaca agttttgggg
     3601 tgaacagcaa ggcgcagacg gatacgtgac gaagcccttc acacgagaag acctgcttcg
     3661 cgcggtgcgg cggtttgcat gagagttttt gaggaaaaga acaggcgatg agcgaagact
     3721 tccatctgga gcctctcccc gcggacgccg ataccggcga tctgctggaa gctcttgggc
     3781 cgatcgaaga gcggccgccg gagtcgcagt attgcgtctt ccgcagcgga cgcgagcgat
     3841 tctgcctccc ggtgctggac gtcgaagaag tgctggattg gccgttgctg acaaagctgc
     3901 cgcttggccc ggcgtacctg gtcggaattt tcaatttgcg cggcgccatc gtgccgttga
     3961 tagacattgc gattacggaa ggccgccggc cgggattact gcccaagcac gtggtggtgg
     4021 cttcgcggcg cagcgagagc catcacgagg accttcgcat cggcatcgcg tccgacgaag
     4081 tcatcggaac gtattcggtg aacaccgagg atttgctcga gcaagcgccg gaaaacgtgc
     4141 cgcattgtat tggaatgttg cgacatgacg accgcctggc gctggtgatc gatttgcgcc
     4201 ggctgctcga ggtttttccg gggcctagca tttgagattt gtggagacaa cgccgatcga
     4261 tggcgagggc tccgcggaag agatttggag ggtggcatga aatcgcgact gagttccaca
     4321 atctggatgt tggtcctgct gaacgcagtg gtcggctgct tcgttctcgg actcgccttc
     4381 agcgccggga agaaggccgg tgacgcgtcc tttttcgact tcggctcgcc cgcaggggga
     4441 accatactct tcgccatcgt actcgcgctg gtcacgtggg gcatcctggc gtggaggttc
     4501 ggtgggttgc tggcgccagt gcaggctctc gcggaattct ccgaacggct ggccgcaggc
     4561 gatccacgcg cacgcgttga ggtcaattcg aatgatgaac tgggctacat cgcggagaac
     4621 ctgaatcgcg cggtcgccag agtctccaag gcaacgagca accaggacgc caacgacgcg
     4681 ctgcagcgca gcatcacgga attgctgagc gtcatcaacc aggtcgcacg tggcgatttg
     4741 gcgcagcgcg gcaaggtgac caacgacgcc cttggaaacg tcacggattc gatcaactac
     4801 atgctcgata acttcaccaa ggttctcgag cgcgttcgca aagcggccat ggaagtgacc
     4861 gcgtgctcca ataacattct ggtggccgct gacgaaatgc aggcaggcgc cacgcagcaa
     4921 gaccaggaaa ttacgaacac gtcgagcgcc gtcgaagagc tgacggtctc catgaaacag
     4981 gtttcgaaca acgccgaagc gagcgccgaa gcggcacgcc gcgcgctgga cgccgctgaa
     5041 cagggcaacc gcgcggtccg cgacacgctc gaaggcatgc agcgcattcg cgcttcagtg
     5101 caggcgaccg ccaagaaaat caaatctctg ggcgaccgct cactggaaat ttccgaaatc
     5161 atcaacgtca ttaacgacat cacggaacag acgaacttgc tggcgttaaa cgcggccatc
     5221 gaggcagcac gcgcgggcga ggcgggacga ggatttgcgg tcgtcgccga cgaagtccgc
     5281 aagctcgccg aacacagccg cagtgcgacg aaggatatcg cggcgctcat caaggcgatt
     5341 caggcggaga cgaacgaagc cgtggtcgtc atggaagaag gcaccaagga agtggaaagc
     5401 ggcgcaaccc ttgccgatca ggcaggccgc gcgctggacg ccatttccaa cgtggtgcgc
     5461 caatccgcgg aactcgttca ggaaatctca ttggcatcga aacagcaggt gcgcggaaca
     5521 gaaggcgtag cgcacgccat gcagatcatt tcgagcatta cgcggcagac gtcgcaagga
     5581 acgcgcggga cggtggcaac ggtaagccag ctcgtcaaat tgtcagatca attgaacgaa
     5641 gcgctggcgc aattccgcgc gtcatcgaaa gcggcggcgg cgcatgaaga ttctgagcgg
     5701 cccgtcccgg caggcgctct tcgttaggca gcgacgctct tcttcgccat ggggcgggag
     5761 taagagcgag gcccagaaaa cgggagcacg atgagcgaac gccagggaca ggtgcaaggg
     5821 ctgagcgaac acgagctgag cgagattcgc atgttgatcg aagagcggac gggaatttgc
     5881 ttcgatgaat cgcgcgagcg cttcttctcc acgcgtgtgc gggaacacct gcacgcaaag
     5941 ggaatggaac gcggcacgga actactgcgc gcgatgcgca aatccaacgt tgaatatgag
     6001 ggcttgctcg agcggctgct gacccaggag acgagcttct tccggtatcc gggcgtgtat
     6061 gaagcattcg agaaacgcgt gctacccgag ctgcacacaa agaaattctg gaagaatccc
     6121 cgtacgctga ggatttggag cgccggttgt tcgacgggcg aagagcctta ttcgatcggc
     6181 atcaccatcg cggattcgct atcgttcgcg gatgcctgga acgtggaaat acttgccacc
     6241 gatgtcgggc ggctcgcgct gaagcaggcc gagcgaggta tctacaaagg ccgcagtatt
     6301 gccagtgtga gcgaaagaca gttggcgaac cactttagcg cggtcagcgg cgggcagcag
     6361 gtaaagccgc ggctacgcaa gttggtttcg tttgcgcaga tgaaccttgc ttcggcggtg
     6421 tacgtgggcc gcatggacat gattttttgc atgaacgtgc tgatttactt ctcggaagag
     6481 cgccggcgct cgctggtgca acggttctac gacacgctgg aaccgggcgg atatctgttc
     6541 ctcgggcact cggagtcgat ctccaagatg cctgtaaagt tccaggctat cgttctaaac
     6601 gattgcattc tttaccggaa accgacagcg gatgaattgc agaaaccaga actcgtgacg
     6661 gagggacgag cgtgagtacg ctggaccgcg aagccgtcga gctgttcctc caggaggcct
     6721 cggagaacct gcaatacttg cgggaatacg tgagtgtgct tcaggacgtt gagccgcgcc
     6781 gagaagacct tgaacggctt tacatctcgg cccacacgct gagcggcact tc
//
LOCUS       DASYVO010000099        14892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_158744, whole genome shotgun sequence.
ACCESSION   DASYVO010000099 DASYVO010000000
VERSION     DASYVO010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 14892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14892
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158744"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..853)
                     /gene="infC"
                     /locus_tag="VGR03_03180"
     CDS             complement(266..853)
                     /gene="infC"
                     /locus_tag="VGR03_03180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV1993313.1"
                     /translation="MAERTFRQSDRIRVNERIRAREIRVIDEEGGQLGVMQPFEAIKL
                     AKEKGLDLVEISPTADPPVCKITDYGKYLYQANKKAHEQRKSSRGSQLKEVKFRPNTA
                     EHDYQVRKNQIIRFLGEGHKVRAMIFHRGREMAHQEVGRAKMTRLLNEIGEHGQIETM
                     PRMEGNVLVALLAPKKGVAVAKPAGQTTSSAQQSQ"
     gene            1120..1485
                     /locus_tag="VGR03_03185"
     CDS             1120..1485
                     /locus_tag="VGR03_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993314.1"
                     /translation="MLSIGSAFTTLERAVLAAICDMYSADRAALEAQLSTAALLSREN
                     TGAGFFTRFAVDRASSVAIGGMRLRDGPATKIDGLEHGMGFILWLKEGYADCLEGYSY
                     AESTTGIVLEAAGFEILQG"
     gene            complement(1596..2321)
                     /locus_tag="VGR03_03190"
     CDS             complement(1596..2321)
                     /locus_tag="VGR03_03190"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1993315.1"
                     /translation="MSQALSRRIFLTLTGGALAAPSVSWAANAEEDDEVPALLDHIIL
                     GCNDLDRGIDLIKENTGVRPAIGGVHPGRGTRNALLSLGERRYLEIIAPDPAQREIVH
                     FPQIREMTNPRLIGWAVHPPDIAATAKQLRENKIAFQGPDDGSRKRPDGRVLNWKTLN
                     LADDRHGLLPFFIEWSADSVHPSKDAPARCRLEYFEIMSTDPDELTLTFKRIGLDLPV
                     QRSDKARLRALISGPKGDLGLST"
     gene            complement(2372..4327)
                     /gene="thrS"
                     /locus_tag="VGR03_03195"
     CDS             complement(2372..4327)
                     /gene="thrS"
                     /locus_tag="VGR03_03195"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV1993316.1"
                     /translation="MEQIHITLPDGSVQEVPKGTTPADIARKISPRLADAALVARVSQ
                     PHDGEGELIDLRRPLDHDVKLQILTEKDPDSLFVFRHSAAHLLAAAVMDLYPNVKLGI
                     GPPIDTGFFYEFLRDEPFTPDDLAKIEKKMHELAAQDLPNERKLIPKPEALDLYKKTN
                     QIFKCELVEEKATEPMVSFYTTGKFIDFCRGPHIPSTKRIQAFKLMNVAGAYWKGQEG
                     NPQLQRIYAVAFFTQKELDAHLHRIEEAKRRDHRKLGTELDLFSIQEEAGPGLIFWHP
                     KGGLIRTIVESWLRDELLKRGYDLVYTPHIMRLDLWKTSGHTDFYKDNMFGAVEVEKT
                     DYQLKPMNCPGHILIYKSKLRSYRDLPVRLAELGTVYRYERSGVLHGLLRVRGFTQDD
                     AHIFCMPSQIESEVEACVDFAFAVMKNFGFDKFEVELSDWDESHPENYAGKPEDWHRA
                     TAALADTMKRMNIPYKKMEGEAAFYGPKIDVKLIDAIGRSWQLTTVQFDFNLPARFGL
                     QYVGDDGAKHQPLMVHRALLGSVERFFGILIEHYAGAFPLWLAPVQVEICPVSEKVAD
                     YAKHVTETLKRHNLRVHLDDRNEKLPAKIRDAQMQKIPYMLVVGPKEAEAGTVSVRHR
                     GKGDLGPKPLAEVVESLQREVDSRATS"
     gene            complement(4436..4831)
                     /locus_tag="VGR03_03200"
     CDS             complement(4436..4831)
                     /locus_tag="VGR03_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEV1993317.1"
                     /translation="MTTPSGSDRYVVDSSGWVEFLGNGPKADAFARYLENPETLLLPT
                     IVVYEVYKKMLREQDVALAERFLSTAFSFQEREIVLDVSLAALAAKRSVTTNLPMADA
                     IIYATAQAHQAELVTSDAHFTGLPGVTLI"
     gene            complement(4809..5021)
                     /locus_tag="VGR03_03205"
     CDS             complement(4809..5021)
                     /locus_tag="VGR03_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEV1993318.1"
                     /translation="METVKVSPKFQVVIPKRVREELQLRPGQELQIYIFEGTIRLNRP
                     GSIKDLRGIAKGMKWKEEDRDHTERF"
     gene            complement(5202..5945)
                     /locus_tag="VGR03_03210"
     CDS             complement(5202..5945)
                     /locus_tag="VGR03_03210"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1993319.1"
                     /translation="MRSSKRPYLRLIAMAYAALLATVSGPPASFPAADDPLGAAVEAA
                     RKTHDEKQLQSLKIQLEQKIAQNPSDAGLYLDLARVQEYFLDVNEMRKDKKAAGESVD
                     KAIDAAQRSIQFNDKSADAHSLLADLYGRKIAYGGMFAGAKFGPKVGEENKKAMALDD
                     KDPRVWASLGRQYLMAPKMFGGDIAKAIESFQKSLAIDPSQDEAWVWLCKALQKQGDK
                     SKAREAVQHALLLNPESRFAQTASASLEK"
     gene            complement(5991..7418)
                     /locus_tag="VGR03_03215"
     CDS             complement(5991..7418)
                     /locus_tag="VGR03_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993320.1"
                     /translation="MRKLLLYFMAIAALIVPASAQWAAATGENPRVFPIGTIVPKVVT
                     LAKPDQSYALYLPASYSAKKRFPIIYALDPGARGSHPVELMKDGAERYGYIVVGSNNS
                     RNGSWKIEIDAAQAMLQDTQERFPIDPRRVYFAGFSGGARVAARLAQLCKCAAGVILN
                     GAEFQPDAFTLHDAPFAVFAGIGIYDFNYGEIVRMDDDLEKLGYAHFLRRFDGPHQWA
                     PSDVMAEALAWFRLQAMKSGLESRDDAWIAATSAQFFEPARALEQSGDLYAAWKEYRQ
                     AAETLAGLTDNRALRAKTEALEKDKAVREGAKREKQEFEEQDQLTREISSDLSGLREI
                     QPNRADVRTGVAQKIVDLRVGAEREKREEKLRVLKRALAGVLVQTMEMGLDRLEQKDP
                     ARARDYFELACDADPDSVWALNNLAVAKALDGDRKGALEALRRAKAKTKDLEQFFAWL
                     NDEPAFAPLRGTPEFKAILEPSAKH"
     gene            complement(7496..8965)
                     /locus_tag="VGR03_03220"
     CDS             complement(7496..8965)
                     /locus_tag="VGR03_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993321.1"
                     /translation="MQNKTDFSIPNVGKTSIAASSRDTRRFSRHLRFASMAVLILLTA
                     TALIVARSSNQFVPRFQEFSDPEGRFANFNPGGPTDTDNNAFFQDLGTNGRRCVTCHQ
                     ASDAWSVIPSHIQSRFNATHGTDPIFRTNDGSGCPTQDVSTEDARRQAYSLLLSKGLI
                     RIEQQVPANAEFIVLHSDNPYGCNSTSAISAYRRPLPSTNIPFLSTIMWDGRETFKDT
                     TGKFQPIPFDLAHQAVDATTGHAQGQPPTAEQVQQIIDFETNIFTAQTHDRRAGDLDE
                     AGAQGGPVHLSRQEFFIGINDPLGLNPTGAAFSSKIFDIYGKWGRIEDRDDDEHTSAR
                     KAVARGENLFNTLPIPITGVAGLNDALHVDTINGFCGTCHDSPNVGDHSVPAPLNIGL
                     VDASRRTPDLPLVTVMCNSTGKIMQVSDIGRAMVTGKCADIGKFKGPILRGLAGRAPY
                     FHNGSAATLLDAVSFYDTRFTLHLSEQDKDDLVAFLKTL"
     gene            9199..11148
                     /locus_tag="VGR03_03225"
     CDS             9199..11148
                     /locus_tag="VGR03_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEV1993322.1"
                     /translation="MPQKSPPPILRFGVFEVNFSAQQLRKHGIRVRLAGQPLAILAML
                     LERPGEVITREEMQRRLWASDTFVDFEHSLNSAIKKLRAALNDTPENSRYIETLPRVG
                     YRFIAPVQEVSEKGHAAADASIAADRSGVLPEVEKSSHRRWPIILGIAIGLIGAGGAY
                     FQWSHFWPRTQRTSGRLMLAVLPFDNLTGDAGQEYFSDGLTEEMIVQLGHLDPLHLGV
                     IARTSVMHYKHGQQQLDQIGRELGVQYVLEGSVRRDANKVRISAQLIQMKDQTRLWAR
                     QYDRELSNLLVLQGEIAQDIAGGIQLTLDDQKRNAPAHQAALSPDAYESHDLYLKGRY
                     FWNKRTIQGFQQAIEYFQQAIAKDPNSARAYAGLADSYALLGGYSMVPQTQLMQKARA
                     AALKALQIDETLAEAHTSLALITENYDRDWQTAEKEYQRAIELNPNYATAHHWYAEYL
                     TWQDRFDEAFRESERARQLDPLSLIIAADNAMILYYSRQYDRAIDKFRAVRKMEPDFP
                     RAEMIINAYVEKGMFAEALAEAENVRRVHGEGPWNWSTLAYVHGRAGQPAEARHALEN
                     LKKQNRHQEIDQAALAWAYIALGDNEQAFGWLEKAYSQHSNAMITLKVEPGYDPLRSD
                     PRFQDLLRRVGLAANGNTGQPTSRP"
     gene            11236..12627
                     /locus_tag="VGR03_03230"
     CDS             11236..12627
                     /locus_tag="VGR03_03230"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV1993323.1"
                     /translation="MSRIARMLVIVFLVMSLPKLAAQEPKALPLPDERYKVDILLVVA
                     HPDDEGAATPYLARALDEHKRVAVVFGTRGSSGANEAGTEQAAALGAIREIEARNALT
                     TLGITNVWFLGGKDTASQNVLQSLANWDHGAALEQLVLLVRLTRPEVILTFLPGTFIG
                     EDHGDHQASGLLATEAFDLAGDPASFPEQLASPTKRLEPFLENLRPWQPKKIYYFPDA
                     DREDIFRGKGPEYSVKEISTVSKQPYWRMALDSFRAHQTQAKSFLDKLAQMDEAQIEK
                     MATSDGGWTDALHFVLGKSLVGGSVTGDVFDGITPGAISFARPDVSPEPARPELLVEL
                     GGPYSFYAEFRRAHGLNNLPHPEPPEIALQAPGTLVIPLWVRNRTAKIQEITLSVVLP
                     AGWTTQSGTGKFTVAAKQVAAARVEVNLPAASENGGKKPDPQEISVHAESNGQTIGEI
                     KLRVELRKRALPQ"
     gene            complement(12634..13488)
                     /locus_tag="VGR03_03235"
     CDS             complement(12634..13488)
                     /locus_tag="VGR03_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1993324.1"
                     /translation="MFQKDLLKGRAVFLTGGGTGLGRSMALRFAELGARIFLVGRREE
                     PLRETCAEIHRAGGAAAYTTCDVRDYGTVEAAAAKAEEQFGEINTLVNNAAGNFMART
                     EKLTPNAFNAVVGIVLNGSFHCTQVFGKRWISQKLGGNVLNIVTTYAAANCGSGFVVP
                     SACAKAGVLAMTTSLAVEWAKYHIRLNAIAPGPFPTEGAWSRLMPSKPFEEHAKDKHP
                     MKRFGRHEELANLAAFLISDMAEYINGECVVIDGAQWLRGAGEFNDLLLLPEAAWEAM
                     EAARHKKS"
     gene            complement(13614..14495)
                     /locus_tag="VGR03_03240"
     CDS             complement(13614..14495)
                     /locus_tag="VGR03_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993325.1"
                     /translation="MRWRTHCLASLLLLSAAVCSARPEADANGPAFTNVPELTSGFHS
                     LYLQNFSEAREKFAGWESQHPDEPFGEVAVAASYLFEELYRQGVLSSDFFLNEKRFLN
                     GIEGKPDPARMKSFQKAIQQARKLARQRLEKDPRDPEGLFALTLSAGMESDADMILKK
                     EHIEALKRLKEANEHAKLLLAEQPDAMDAYVALGAANYIIGSLSGGARFMLWFGGIHG
                     DKKLGMEQLGHTIEKGRYLQPYAKILLALAARREKQNPLAQKLLRELSEEFPESPLYA
                     AEYAKAMGRPIPAQIHP"
     gene            complement(14510..>14892)
                     /locus_tag="VGR03_03245"
     CDS             complement(14510..>14892)
                     /locus_tag="VGR03_03245"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEV1993326.1"
                     /translation="LLLRRPFLPALAAVTFTIFLAATAVRWWVISTLGEHWNVQVLDS
                     TQLGVVTSGPFRFVRHPNYAAVFSEMLALPLIHTAWITALAGALAHVAVLAMRLSTEE
                     RVLFANPDYRAAMAGKPRFFPGLF"
BASE COUNT         3030 a         4160 c         4498 g         3204 t
ORIGIN      
        1 agcttcttga gcttgcgcat gcggccgggc ctcttggtgc ccatcaaatg ccgcttgccg
       61 gagtgcatgc gcttgatctt gccgctcgcg gttattttaa agcgcttcct ggcgccgcgg
      121 tgggtcttca atttcaattt gggcttcgat ctgtttctcg gcattgcttg aaatcctctc
      181 ctgagattcg gcggtgacac cgaggagggt gcccgccgcg gctggcgccc ctacatgatt
      241 tttctgatgc gttcttttcc gccaattact gcgactgctg tgcgctggac gttgtctgcc
      301 ctgcgggctt tgccacggcc acgccttttt tcggagccag cagagccacg aggacgttgc
      361 cttccattcg cggcatcgtc tcaatctggc cgtgctcgcc gatttcgttg agcaaacgcg
      421 tcatcttggc gcggcccact tcctggtggg ccatttcgcg cccgcgatga aaaatcatcg
      481 cgcgaacttt gtggccttcg ccgaggaagc ggatgatctg gttcttgcga acctgatagt
      541 cgtgttccgc ggtgttgggc cgaaacttga cttccttcaa ctgcgagccg cggctgctct
      601 tgcgctgctc gtgggccttt ttgttggcct ggtaaagata tttgccgtag tcggtgatct
      661 tgcaaaccgg cggatcggcc gtgggcgata tttccaccag gtcgagcccc ttttccttcg
      721 ccagctttat ggcttcaaac ggctgcatga cgccgagctg gccgccttct tcatcaatca
      781 cccggatctc gcgtgccctg attcgctcgt taacgcgaat gcggtcgctt tgccggaaag
      841 tccgttctgc gataagtccc cctcaagcac ttcgtacttc gcggccacca agcggccgac
      901 aggcacctgc tttccccggt acatccaggg gctggccccg attcgagaat tcaatcatca
      961 cgcgaagtga tgaacccacc ccacacggcg caagagagcc aggggacggg cttcgaagta
     1021 tagcacgtgg ccgcggctgc acggcaagag gcaaaagggt gggtagtggg tcagtttgaa
     1081 agtcatcgtc gaagcggtgt tcaggctatg atgcgacaca tgctatcaat cggatcggcc
     1141 ttcaccacac tcgaacgagc agtgctcgcc gcgatttgcg acatgtattc agcggatcga
     1201 gcggcgcttg aggcgcaact atcgactgcg gcccttctca gccgcgaaaa cactggcgct
     1261 ggttttttca cccgtttcgc ggttgaccgc gcctccagcg ttgccattgg aggtatgcgc
     1321 ttaagagacg gcccagcaac taagatcgac ggattagaac acgggatggg gtttattctc
     1381 tggctgaaag aaggctacgc agactgcctt gaaggatatt cctatgcgga gagcacaacc
     1441 ggtatcgtcc tagaagcagc aggtttcgag atactccagg gttaggacga agctgctggt
     1501 catgccagac tggcccacta ccgaagggtg tggcgccgag attcagtcga gagttatgaa
     1561 tcggagcaga tgccgcttga atcgaagcgc gaggctcagg tgctcaggcc caggtcgccc
     1621 tttggcccgc tgatgagcgc gcggagtcgg gctttgtcac tgcgttggac gggaaggtcg
     1681 agcccgatgc gtttgaaggt caacgtgagt tcgtccggat ccgtgctcat gatctcaaaa
     1741 tactccaagc ggcaacgcgc gggagcatcc ttggaggggt ggaccgagtc cgcgctccat
     1801 tcgatgaaga aggggaggag gccgtggcgg tcgtcggcga gattgagggt tttccagttg
     1861 aggacgcggc catccgggcg ctttctggag ccgtcgtccg ggccctggaa cgcaatttta
     1921 ttttcgcgga gctgcttggc ggtggcagcg atgtcgggag gatggacagc ccagccgatg
     1981 aggcgcgggt tggtcatttc acggatttgc gggaagtgga cgatctcccg ttgggcgggg
     2041 tccggcgcga tgatttccag atagcgacgc tctccgagcg agaggagtgc gttgcgcgtg
     2101 ccgcggccgg gatggactcc gccgatggcg gggcgcacgc cggtgttttc cttgatgaga
     2161 tcgatgccgc gatcgagatc gttgcagccg agaatgatgt gatcgagcaa agcgggcacc
     2221 tcatcgtcct cttccgcgtt cgccgcccag gatacagatg gcgcggcaag tgcgccgcca
     2281 gtcagcgtga gaaaaatacg ccgtgagagc gcttgtgaca ttcgtgacct cacgtgagca
     2341 gggcattcaa atggcgacgc gcgaactcga cttagcttgt ggcgcgggaa tcgacttcac
     2401 gctggagcga ttcgacgact tcggcgaggg gcttggggcc gaggtcgcct ttgccgcggt
     2461 ggcggacgga gacggtgccg gcttcggctt ccttggggcc gactaccagc atgtagggaa
     2521 ttttctgcat ttgcgcgtcg cggatttttg cgggcagctt ttcgttgcgg tcgtcgaggt
     2581 ggacgcggag gttgtggcgc ttcagcgttt cggtaacgtg cttggcgtag tcggccactt
     2641 tttcgctgac cgggcagatt tccacctgca ccggcgcaag ccacagcggg aaggcgcccg
     2701 catagtgctc gatcaagatg ccgaagaagc gctcgacgga gccgagcagc gcgcggtgca
     2761 ccatgagcgg ctgatgcttg gcgccgtcgt cgccgacgta ttgcaggccg aagcgcgcgg
     2821 ggagattgaa atcgaactgc acggtggtga gctgccagga acggccgatg gcgtcgatga
     2881 gcttcacgtc gattttcggg ccgtagaagg cggcttcgcc ttccattttt ttgtacggga
     2941 tgttcatgcg cttcatggtg tcggcgagag cggcggtggc gcgatgccag tcttcgggct
     3001 tgccggcgta attctccgga tgcgattcgt cccaatcaga gagttcgact tcgaatttgt
     3061 cgaaaccgaa atttttcatg acggcgaagg cgaagtcgac gcaagcctcg acttcggatt
     3121 cgatttgcga gggcatgcag aagatgtgcg catcgtcttg cgtgaagccg cgcacgcgga
     3181 gcaagccgtg gagcacgcca ctgcgttcgt agcgatagac ggtgccgagc tcggcgaggc
     3241 ggacgggaag gtcgcggtag ctgcggagtt ttgatttgta aatcaaaatg tggccggggc
     3301 aattcatggg cttgagctgg tagtcggttt tctcgacttc caccgcgccg aacatattgt
     3361 ctttgtaaaa atccgtgtgg ccgctggtct tccagaggtc gaggcgcatg atgtgcggcg
     3421 tgtagacgag gtcgtagccg cgcttcagca gttcgtcgcg cagccagctt tccacgatgg
     3481 tgcgaatcaa tccgcccttg ggatgccaga aaatcagtcc ggggccggct tcttcctgaa
     3541 tgctgaagag gtcgagttcc gtgccgagct tgcggtgatc gcggcgcttg gcttcctcga
     3601 tgcggtgcaa gtgcgcgtcc agctcttttt gggtgaagaa agccaccgcg taaatgcgct
     3661 ggagctgggg attgccttcc tggcccttcc agtaagcgcc ggcgacgttc atcaacttga
     3721 acgcctggat gcgcttggtg gaaggaatgt gcggaccgcg gcagaagtcg atgaatttcc
     3781 cagtggtgta gaacgagacc atcggctcgg tggctttttc ttccacgagt tcgcatttga
     3841 aaatctggtt ggtctttttg tagaggtcga gagcttcggg tttgggaatc agcttgcgct
     3901 cattgggcaa atcctgcgcc gcgagctcgt gcatcttctt ttcgattttg gcgaggtcgt
     3961 ccggagtgaa gggctcatcg cgcaggaatt cgtagaagaa gccggtgtcg atcggtggac
     4021 cgatgccgag cttcacattc ggatacaagt ccatcaccgc cgccgcgagt aggtgcgctg
     4081 cggaatggcg gaacacaaaa agcgaatcgg gatccttttc ggtgaggatt tgcagcttga
     4141 cgtcatggtc gagcggacgg cgcaggtcga tcagctcgcc ttcgccatcg tggggctgag
     4201 agacacgggc gacgagcgcg gcatcggcga gacgaggcga aatcttccgc gcaatatcag
     4261 caggcgtagt gcccttcggc acttcctgaa cgctgccatc cggcaatgtg atgtgaatct
     4321 gctccatgtt cgtattcacc tggaaggaga aagatcaagc ttatcggcgg cttccaaccg
     4381 ggtcaaggcg tgccagacga atcgcgggca tgtcgaagcc aagcacagtc tgaaactaaa
     4441 tcagcgtcac tcccggcagc ccggtaaagt gcgcgtccga ggtgaccagc tctgcttggt
     4501 gggcttgcgc tgttgcgtag atgatcgcgt ccgccatcgg caagttcgta gtcacgctcc
     4561 tcttagctgc caaagcagct agggagacgt cgagaacaat ttcacgttcc tgaaagctga
     4621 aagcagtgga cagaaacctc tccgccagag ccacgtcttg ctcgcggagc atcttcttat
     4681 agacctcata tacaacgatg gtgggaagta gcagggtctc cgggttttcg agataccttg
     4741 cgaacgcgtc ggctttagga ccgttgccaa gaaactcaac ccaaccagaa gaatccacaa
     4801 cgtatcggtc agaaccgctc ggtgtggtca cggtcctctt ccttccactt catgcccttg
     4861 gcaatgccgc gaagatcttt aatggacccc ggccgattta gccggatggt gccttcgaaa
     4921 atgtaaatct gtaattcctg gccgggccgt agctgaagtt cttcgcgcac ccgctttgga
     4981 atcaccactt ggaatttggg agacactttg acggtttcca tggattggtc ctaagccaag
     5041 tctatcgata ccactagtgt atgtcaatag tttaatcgat ataatagtgt aatatgaatg
     5101 ccacatcgat ataatagcgt atgtcacaat ctatatcgat tgtgggccaa agtgaaaaaa
     5161 aggaggctgc aaactagttt gggtagcaaa ccaaatcttt cctatttctc caggctggcg
     5221 gacgcagtct gcgcaaaacg ggattccgga ttcaacaaca aagcgtgctg caccgcctct
     5281 cgcgctttgc tcttatctcc ctgtttttgc aatgctttgc agagccaaac ccaggcttcg
     5341 tcttgcgaag gatcaatggc cagggacttt tggaaactct cgatggcttt ggcgatgtcg
     5401 cctccaaaca tcttcggagc catcagatac tgacgcccca aactggccca cacgcgggga
     5461 tccttgtcgt ccagggccat cgcctttttg ttttcctctc cgaccttggg gccaaatttc
     5521 gcacctgcga acatcccgcc gtaggctatc tttctgccgt aaagatcggc aagcaggcta
     5581 tgtgcatcgg ccgacttgtc attgaactgg atggaccgct gggccgcgtc aatggcctta
     5641 tccaccgatt ctcccgcggc cttcttgtct ttgcgcattt cattgacgtc caggaaatat
     5701 tcctgaactc tcgccagatc aagatagagg cccgcatcgc tggggttctg agcgatcttc
     5761 tgttccagtt gtatcttcag ggattgcaat tgtttctcat cgtgggtttt cctggcggct
     5821 tcaacggcgg cgccgagagg atcatccgct gcgggaaaag acgcaggcgg ccctgagaca
     5881 gtggcgagta gagcggcata cgccattgcg ataaggcgga gataaggcct tttcgatgaa
     5941 cgcataggca agtccctgct tcttcctacg ggtggaacac ggcgaaactt tcagtgtttc
     6001 gcggatggtt cgaggatggc cttgaattct ggagtgcctc ggagtggcgc gaacgcgggc
     6061 tcgtcgttta gccacgcgaa aaattgctca aggtccttcg ttttggcttt cgcgcggcgc
     6121 aacgcttcca gcgccccctt gcgatctcca tccaacgctt tggctaccgc caaattgttc
     6181 agcgcccaca cggaatccgg atcggcatcg catgccagct caaaatagtc ccgcgcgcgc
     6241 gccggatctt tttgctccaa acgatccagc cccatttcca tcgtctggac cagcacacct
     6301 gctagagctc ttttcaacac gcggagtttc tcctcgcgtt tttcgcgctc cgcgccgacg
     6361 cgcaaatcga cgatcttctg cgccacgccc gtgcggacat ccgcgcgatt gggctgaatt
     6421 tcccgcaacc cggagaggtc cgaggaaatc tcgcgggtca gctgatcctg ttcctcgaac
     6481 tcctgctttt cgcgtttggc cccttcccga acggctttgt ccttctcgag ggcttcggtc
     6541 tttgcgcgca gcgccctgtt atccgtcagt ccggcaaggg tttctgcagc ttgtcggtat
     6601 tctttccagg cggcgtagag gtcgccggac tgttcgagcg cgcgagccgg ttcaaaaaac
     6661 tgcgcggaag ttgcagcgat ccaggcatca tcccggcttt ccaggccgct cttcatcgcc
     6721 tgcaaacgga accacgccag tgcttctgcc atcacatcag acggagccca ttgatgcgga
     6781 ccgtcaaagc ggcgcaggaa atgggcgtag ccgagttttt ccaagtcatc gtccattcga
     6841 acgatttcac cgtagttgaa atcgtatatg ccgatgccgg cgaagaccgc aaagggcgcg
     6901 tcgtgtaaag tgaaagcgtc cggctggaat tccgctccat tgaggatcac gcccgcggcg
     6961 catttgcaga gttgcgccag ccgcgccgcc acgcgcgcgc ctccggaaaa gccggcaaaa
     7021 tacacgcggc gcggatcgat cggaaatctc tcctgggtgt cctgcagcat ggcttgcgcg
     7081 gcatcaattt cgattttcca ggagccattg cgggaattgt ttgagcccac cacgatgtag
     7141 ccgtagcgtt ccgctccatc tttcataagt tcgacagggt gactgccgcg agcgcccgga
     7201 tcaagtgcgt aaattatcgg gaaacgcttc ttggccgagt atgacgctgg gagatacagc
     7261 gcgtaacttt ggtctggctt tgctagggta accactttcg gaacaatcgt cccgatgggg
     7321 aagacccggg gattctcgcc agttgcggca gcccactgcg cggaggcagg gacgataaga
     7381 gcggcgatgg ccataaaata cagaagcagt ttgcgcacgc tcctactata gacgcgccaa
     7441 ccgagacttg ggagagcccc ggctggcgcg tgttctgccg ttattcccga acccgctaaa
     7501 gtgtcttcag gaaagcgaca aggtcatcct tgtcctgctc cgaaaggtga agagtgaacc
     7561 gcgtgtcgta gaagctgacg gcatccaaca aggttgccgc ggagccgttg tggaaataag
     7621 gcgcgcgccc tgcgagaccc cgcaggatcg ggcccttgaa cttgccgatg tccgcacact
     7681 tgccggtgac catggcgcgg ccgatgtcag agacctgcat gatcttcccg gtcgagttgc
     7741 acatgaccgt gacgagaggc aggtccggtg tgcggcgaga agcatcaacg aggccaatat
     7801 tgagcggcgc cgggaccgag tgatcgccaa cgttcggcga atcgtggcaa gtgccgcaga
     7861 agccgttgat ggtgtccaca tgcaaagcgt cattgaggcc ggcgacgccc gtgatgggga
     7921 tgggcagggt gttaaacaga ttctcgccgc gagctacagc cttgcgcgca gaggtgtgtt
     7981 cgtcatcgtc gcgatcctcg atgcgtcccc attttccgta gatgtcgaag atcttggagg
     8041 agaatgcggc accggtcgga ttcaatccga gagggtcatt gatgccgatg aagaactctt
     8101 gcctggagag atggacaggg ccgccctgcg caccggcttc gtctaaatcg ccagcgcggc
     8161 gatcatgggt ttgcgccgtg aagatattgg tctcgaagtc gatgatttgt tgcacctgtt
     8221 cggcggtcgg gggctgacct tgagcgtggc cggtggtggc atccaccgct tgatgagcga
     8281 ggtcgaatgg aatgggttga aacttgccgg tggtgtcttt aaacgtttcg cggccgtccc
     8341 acatgatggt gcttaggaag ggtatgttag tggacggcag cggacggcga taggcagaga
     8401 tcgccgatgt gctgttgcag ccgtaagggt tgtcgctgtg gagcacgatg aactcggcat
     8461 tggcgggaac ttgttgctcg atgcggatga ggcctttgct caacaacaag ctgtaagcct
     8521 ggcggcgtgc gtcttccgtg gacacatcct gggttgggca gccggagccg tcattcgtcc
     8581 ggaagatcgg atcggtaccg tgggtggcgt tgaaacgtga ctgaatatgc gacggaataa
     8641 cagaccaggc gtcgctggct tgatgacaag tgacgcatcg ccgtccgttc gttcccaggt
     8701 cctggaaaaa ggcgttgttg tccgtatcgg tcgggccgcc ggggttaaag ttggcaaaac
     8761 gtccctcggg gtcagagaac tcctgaaaac gggggacgaa ttggtttgaa cttctggcga
     8821 cgatcagagc ggttgccgtc aagaggatga gaacagccat tgaagcaaag cggagatgcc
     8881 gggagaaacg tcgagtatct ctcgagcttg ctgcaatact tgtttttcct acatttggaa
     8941 tagaaaagtc agtcttgttt tgcattcgtt cgaacctttc aagagcgcag tgagtcggcg
     9001 tacgggagat cgcatcccct tggagacccg ggaggagcga atgtaagccg agagtatgat
     9061 tagtagtgat ccgcaagcaa tgcccgtagc gtgaaacatt gatgaccagc caagtttatg
     9121 ataaaaatat agttatccat cttggtgacc gcggtgcatc cccgcgccca gaatggcaac
     9181 cgttcccagg gagctgaagt gccgcaaaag tccccaccgc cgatcctgag gtttggcgtc
     9241 ttcgaggtca atttttcggc gcagcaactg cgaaagcacg gcatccgcgt gcggcttgcc
     9301 ggtcaaccgc ttgctatttt agcgatgctc ctggagaggc ccggggaagt gattacccgc
     9361 gaggaaatgc agcggaggct gtgggcttcc gacactttcg tggattttga gcacagcctg
     9421 aacagtgcga tcaagaagct gcgcgcggcg ctgaatgaca ccccggaaaa ttcacgctac
     9481 atcgagacgc ttccgcgagt gggctaccgg tttatagcgc cggtgcagga agtctcagag
     9541 aaggggcacg ccgcggcgga cgcctcaatt gctgccgacc gcagcggcgt tctgcctgag
     9601 gtggaaaagt cgtcccaccg ccgatggccg atcattcttg gtattgccat cggcctgatt
     9661 ggagccgggg gtgcgtactt tcagtggtcc catttctggc cgcgaactca acggacgagc
     9721 ggaagattga tgctggcggt tctgcccttc gacaatctca ccggagacgc cgggcaggag
     9781 tacttcagcg acggcctgac cgaggaaatg atcgtccaac tcggacacct cgatccgcta
     9841 caccttggag ttattgcgcg tacctccgtc atgcattaca agcatggcca gcagcaattg
     9901 gatcagatcg gccgtgaact gggagtccag tacgtgttgg aaggcagcgt ccggcgcgat
     9961 gcgaacaagg tgcgaatctc agcccagttg attcaaatga aagaccagac ccgtttatgg
    10021 gcgcggcagt acgatcgtga actgagcaac cttttggttc tccaaggcga gattgcgcaa
    10081 gatatagctg gcggaattca actcaccctc gacgaccaga agaggaacgc ccctgcgcat
    10141 caggccgctc tctctcccga tgcctacgag tcccatgacc tgtacctcaa gggccgctac
    10201 ttttggaaca agaggactat tcaagggttt cagcaagcta tcgagtattt tcagcaggcc
    10261 atagcgaaag accccaattc tgcccgcgcc tatgccggcc tggcggattc ctacgccctg
    10321 ctgggcggat acagtatggt tccacaaacc cagctcatgc agaaggcgcg cgcagcggcg
    10381 ctcaaggcgc tacagattga tgagacgctc gccgaagccc acacctcgtt ggcattgatt
    10441 accgagaatt acgaccggga ctggcaaacg gcggaaaaag aataccagcg agcaattgag
    10501 ttgaacccca actacgccac ggcccaccat tggtacgcgg agtacctgac ctggcaggac
    10561 cgtttcgatg aggcgttccg cgagagcgag cgggcccggc agcttgatcc actctcgctg
    10621 atcattgcgg cggacaatgc catgatcttg tactactcgc gacagtatga ccgcgccatc
    10681 gacaaatttc gagctgtgcg caaaatggaa ccggactttc cgcgtgccga gatgatcatt
    10741 aatgcgtacg tcgaaaaggg aatgtttgct gaagcgttgg ctgaggccga gaacgtgcgc
    10801 cgagtacatg gtgaggggcc ctggaactgg tcaacgctag cctacgtcca tggccgcgcg
    10861 ggccagccgg cggaggctcg acatgcgctg gagaatttga aaaagcagaa ccggcaccag
    10921 gagatcgatc aggccgcgct tgcctgggca tatatcgcgc tgggcgacaa cgagcaggcg
    10981 ttcggctggt tggaaaaggc atactcgcag cattccaacg caatgatcac ccttaaggtg
    11041 gagcccggct acgacccgtt gcgcagcgat ccacgtttcc aagacttatt gcggcgcgtc
    11101 ggattggcag caaacgggaa caccgggcaa cccacctcaa ggccgtgacg cttaatccgt
    11161 cagagatcaa gatgacggtg caaggcaggc ccaagtcact agcgatcatt gctatcattc
    11221 atttgaggaa actacatgtc gcgaatcgcg aggatgttgg tcattgtttt tctagtcatg
    11281 tcgttgccaa agctggcggc gcaggagccc aaagcgcttc cgctgccgga cgagcgctac
    11341 aaagtggaca tcctcctcgt tgtggcgcat cccgatgatg agggcgcggc tactccttat
    11401 cttgcgcgcg cgctggacga acacaaacgc gtggctgtcg tctttggaac gcgtggcagc
    11461 agtggcgcga atgaagccgg gacggagcaa gcggcggcgc tcggcgcgat tcgggagatt
    11521 gaagcgcgca acgcgctcac aacgctgggc attaccaacg tgtggtttct cggcggcaag
    11581 gacacggcat cgcaaaatgt attgcagtcg ctggcgaact gggatcacgg cgctgcgctc
    11641 gaacaactcg tgctcctcgt gcgactgact cgcccggaag tcatcctgac tttcttgccg
    11701 ggaacattta ttggcgaaga ccatggggac catcaagcct ccgggctgtt ggcgacggaa
    11761 gcattcgatc tagcgggcga tcccgcgtcg ttcccggagc agctagccag cccgaccaaa
    11821 cgcctcgaac cctttcttga gaacctgcgg ccgtggcagc caaaaaagat ctattacttt
    11881 ccggatgcgg accgcgagga tatttttcgt gggaagggcc ccgagtattc ggtgaaggag
    11941 atttccacag tgagtaaaca gccgtactgg cggatggcgc tcgattcgtt ccgcgcccac
    12001 cagacgcagg ccaagtcctt tctcgataag ctcgctcaga tggacgaggc acaaatcgaa
    12061 aagatggcca cctccgatgg cggctggacg gacgcattgc acttcgtgct cggcaagtcg
    12121 ctggtcggcg gcagcgtgac cggagatgtt ttcgatggca tcacaccggg tgcgatttcc
    12181 ttcgcccggc ccgatgtttc tcctgagcct gcacggccgg aattattggt ggagctcggc
    12241 ggcccgtaca gtttctacgc agagtttcgg cgcgcacatg ggctcaacaa tcttccacat
    12301 cccgagccgc cggagatcgc gctgcaggct cccggtacgc tggtgattcc attgtgggtc
    12361 cgcaatcgaa ccgcgaaaat ccaggagatc acgctctcag tagttctgcc cgcggggtgg
    12421 accacgcaaa gcggcaccgg gaagtttacg gtggcggcga agcaagtggc ggccgctcgc
    12481 gtcgaagtga atctgccggc cgcctcggag aatggcggga aaaaaccgga tccgcaggaa
    12541 atcagcgttc atgcggaatc gaatgggcaa accatcgggg agatcaaact tcgcgttgaa
    12601 ctgcggaaac gggcgctgcc gcagtaggct gcttcaggat tttttgtgac gcgcggcttc
    12661 catcgcttcc caggcagctt ccggcagcaa cagtagatcg ttgaattcac cggcgccgcg
    12721 cagccattgc gcgccgtcaa tcacgacgca ttcgccgttg atgtactccg ccatgtcgct
    12781 gatgagaaat gcggcgagat tggccagctc ctcgtggcgc ccgaagcgct tcatgggatg
    12841 cttgtccttg gcgtgctctt cgaatggttt cgaaggcatc agccgcgacc acgcaccttc
    12901 ggtggggaac ggccccggcg cgatggcgtt tagccggatg tgatactttg cccattccac
    12961 agccagcgag gtggtcatcg ccaaaacgcc cgctttcgcg caagccgatg gcacgacaaa
    13021 ccccgagccg cagttggcag ccgcataggt ggtcacgata ttcagcacat tgccgccgag
    13081 tttttgggaa atccagcgct tgccaaatac ctgcgtgcag tgaaatgaac cgtttagcac
    13141 gatgccgacg acggcgttga atgcattggg agtcagtttt tccgtgcgcg ccatgaaatt
    13201 tccggcggcg ttgttgacca acgtgttgat ttcgccgaat tgttcctcag cctttgctgc
    13261 cgcggcttcg acggtgccat agtcccgcac gtcgcaagtc gtgtacgcag ctgcaccgcc
    13321 cgcacggtga atctcggcgc acgtttctcg cagcggctct tcccgccggc cgacgagaaa
    13381 gatccgcgcg cccaactccg caaaccgcaa cgccatcgat ctgccgagtc ccgttccccc
    13441 gccggtaagg aacactgccc ggcccttcaa caagtccttt tggaacacgt ttcaccgcct
    13501 cgtgcagatg actgcgcgcg tcaatcgcgc agcgcgattc taatccatcc gagagaatgc
    13561 ggatgatttt ttcaaatggc gcccggctgc aagcggaagg aatgcaaaca agcttaggga
    13621 tgaatttgcg cgggaatggg ccgtcccatc gctttggcat actcggcggc gtacagcggg
    13681 ctttccggaa attcctcact taattcgcga aggagtttct gcgccaaagg gttttgtttt
    13741 tctctgcgcg ccgccaatgc cagcaagatc ttggcatacg gttgcaggta ccgtcccttt
    13801 tcaatggtgt gtccaagctg ctccatgccg agtttcttgt cgccgtgaat tccaccgaac
    13861 cagagcatga agcgcgcgcc gccggagagg cttccgataa tgtagttggc cgctcccagc
    13921 gcgacatacg catccattgc atcgggctgc tccgccagca acagcttggc atgttcgttg
    13981 gcttccttga gccgcttcaa ggcctcgata tgctccttct tcagaatcat atcggcatcc
    14041 gattccattc ctgccgacaa ggttagggca aagagccctt ccggatcgcg tgggtctttc
    14101 tccaggcgct ggcgggcgag ttttctggcc tgctggatgg ccttctgaaa actcttcatg
    14161 cgcgcgggat cgggcttgcc ttcaatgccg ttgaggaatc ttttttcgtt caagaagaag
    14221 tcgctggaga gaacgccctg gcgataaagc tcctcgaaga ggtaacttgc cgcaaccgca
    14281 acctcgccaa aaggctcgtc gggatgctgc gattcccagc cggcaaattt ttcgcgcgcc
    14341 tcggagaagt tctggagata gagggaatga aatccagagg tgagttccgg cacgttggta
    14401 aacgccggac cattggcgtc agcctccggc cgcgcactgc agacggcggc ggatagcaac
    14461 agcagggatg ccagacaatg ggttcgccaa cgcattctga acctgctctt cagaaaagcc
    14521 cgggaaagaa gcgcggtttg cccgccattg ccgcgcggta gtccggattt gcaaaaagta
    14581 cacgctcttc cgtggagagc cgcatcgcca gaaccgccac gtgcgcgagc gctcccgcga
    14641 gcgcggtgat ccaggccgtg tggatcagcg gcagcgccag catctcggag aacaccgctg
    14701 catagttggg atgccgaacg aagcgaaacg gtcccgacgt gaccactccc aactgcgtcg
    14761 agtccaatac ttgcacgttc cagtgttcgc caagcgtgct gatcacccac cagcgcacgg
    14821 cagtcgccgc cagaaaaata gtaaacgtca ccgcagccag cgccggaagg aacggccttc
    14881 ttaagagcag ga
//
LOCUS       DASYVO010000100        10305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_142430, whole genome shotgun sequence.
ACCESSION   DASYVO010000100 DASYVO010000000
VERSION     DASYVO010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 10305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10305
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142430"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(544..1053)
                     /locus_tag="VGR03_03250"
     CDS             complement(544..1053)
                     /locus_tag="VGR03_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEV1993327.1"
                     /translation="MYGKPRQLETEAELYDVAVRALMRRAHSVHEMKRKLERRSDNKL
                     LVQLVMARLKENGQIDDAKYAKQFARQRTETRKQGKFRISRDLRARGVPDRHIEAALE
                     EAAKETDEGAMVRQRIERKLRSYRGEIDEKKMASMYGSLLRAGFSADVVRRELKALTR
                     EDVPEAGSE"
     gene            complement(1185..2330)
                     /locus_tag="VGR03_03255"
     CDS             complement(1185..2330)
                     /locus_tag="VGR03_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEV1993328.1"
                     /translation="MAVSIDDLLRRAVESKASDLHLKVGNHPYLRVDGLLAPLTDVPR
                     VTPEEMLSMAFSMMTNRQKQKFKETAELDMAYGVAGLGRFRVNVFQQRGNVGMVLRVI
                     PTKIRTTEELNLPPVISKICEEQRGLVLVTGTTGSGKSTTLAAMIDRINANRADHLIT
                     IEDPIEFLHRDKKSFINQREVEVDTANFSTALRAALRQDPDVILVGEMRDLETISTAL
                     LAAETGHLVLSTLHTLDATETIQRIIAVFPPPEQKQIRLQLAATLKAVVSQRLVRRAD
                     DKGRVPACEIMIATGYIRDCIINADKTRLIHDAIAAGTSQYGMQTFDQSLFDLYSKQL
                     ITLDEALARASNPDEFKLRIQGIRSAADSAREEMASQMADFERFARK"
     gene            complement(2468..3157)
                     /locus_tag="VGR03_03260"
     CDS             complement(2468..3157)
                     /locus_tag="VGR03_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HEV1993329.1"
                     /translation="MYSTSQKLVAEFLGTFALIFFGVGSICTDQYLHGAGGGLGLLGI
                     ALAHGLAIAIMVSALGHISGGHFNPAVTIGFWVTKRLGTIEAFLFWVAQLAGASAAAF
                     LLKTVVPEETWRAVSLGTPALARDFPVWAGISLEAVTTFFLVLVVFATAVDEKGAFRS
                     IAGFGIGLTISLGFLVAGPLTGAALNPARAFGPALAATHWAHQGVYWVGPLAGGFVAG
                     LLYDSLYLKKA"
     gene            complement(3279..5687)
                     /locus_tag="VGR03_03265"
     CDS             complement(3279..5687)
                     /locus_tag="VGR03_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV1993330.1"
                     /translation="MIGQTLSHYRILEKLGGGGMGIVYAAEDFKLGRRVALKFLPEEL
                     NKNPQALERFRREARAASSLNHPNICTFHDIEEHEGRFFLVMELLEGETLKARVTGRP
                     LAMDALLDVGIQVADALDAAHKKGIVHRDIKPANIFITTRGQAKLMDFGLAKLNPDAS
                     SPGTTLGSLEGVTTEEALTSPGIAMGTVAYMSPEQARGEELDARTDLFSFGAVLYEMA
                     TGRAAFSGPTSAVIFHAILERTPPNPSTANSNLPSRFDEIVSKALEKDRTLRYQSAAE
                     ISADLKRLKRDSDSARVPSAPPATAPRRRMSLLAKVAIVSGTLVILAGIAIVARHGFQ
                     EIQLKSMDSVAILPIVNATGDSNSEYLSDGITQDLVSTLSQLPNLKVVSLASAYRYKG
                     KGIDPPSVARELGVHTIFTGRMVQRGDDLSISAEFVDAEHDRVMWSKQYHWKLADVPN
                     IQTEITQDITENLKMNLTGAQKSRLSQRPTENGEAYQLYLRGRFYWNKRNAEDVSKAT
                     SYFQRAIERDPNYALAYAGLADCYYVTFFKNFTISQAESAAKSRAAATKALELDPSLA
                     ETHVSMGQVLAIFDWDFAGAEREFRRALVINPGYAIGLQWYAELLENLARYDEALALI
                     RRAQDADPFSLIINAVHGQILSRSGRTNEAIAQFQKTLDLERNFSLGRFLFGLTLVHG
                     GKNEEAVKELEQAVQSSPESPVYRSMLAYVYAQTGRIEEARKILGDVIQEAKSDRASW
                     QDVAGIYAALGEKDHAFAALEMAFQRRDRRMTMLLDHEALMPLRSDPRFSDLVRRVGL
                     PQHN"
     gene            complement(5871..6263)
                     /locus_tag="VGR03_03270"
     CDS             complement(5871..6263)
                     /locus_tag="VGR03_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEV1993331.1"
                     /translation="MILLDTHVVLWMTSDPAWLSAKARESIEISRKNGDGLAICDITL
                     LELTTLASKGRIHLGISLESFLQEVEARFVVFPITGRACARAMAFPATYPKDPADRII
                     GATALVEGLSLITADREIRRSKLIPTIW"
     gene            complement(6260..6493)
                     /locus_tag="VGR03_03275"
     CDS             complement(6260..6493)
                     /locus_tag="VGR03_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEV1993332.1"
                     /translation="MKKMAAGAFKANCLAVMDEVQSKRVTVVITKHGKPVAKLVPVGP
                     QVDDIFGFLRGKVTITGDVVSPALLARDWGHLW"
     gene            complement(6710..7657)
                     /locus_tag="VGR03_03280"
     CDS             complement(6710..7657)
                     /locus_tag="VGR03_03280"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEV1993333.1"
                     /translation="MPIQATEKIWHNGKWINWDDAKLHVLSHVVSYGSAVFEGIRCYE
                     TKQGPAIFRLRKHMQRLINSAKIYRMELPFSVDQFSNTACELVRLNKLNSCYVKPIVL
                     SGYGEVGVNPLNSPIDIYMACWSWGAYLGPEALSKGVEVGVSSWTRIAPNTLPAMSKA
                     AANYMNSQLIRMEATFNGYTEGIALDSGGHVSEGSGMNVFLVHDGTLYTPPLATSILP
                     GITRDAIVKLAEDLNIPVKETVIPREMLYIVDEVFFVGTAVEVTPIRSVDHIQVGKGV
                     AGPVTRKLQEEFFAITSGGKPDRHNWLTPVNAPVGATSR"
     gene            complement(7820..8725)
                     /locus_tag="VGR03_03285"
     CDS             complement(7820..8725)
                     /locus_tag="VGR03_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1993334.1"
                     /translation="MTNFATLTRMRIQLTLRNKMFLFFSVIMPFGFFFLYAGVFAKGI
                     PLVVRYFLGPVIALTVMGSFWGLSAALVMFREQGILRRFHVTPVTPSDMLASSVVANF
                     VLTLPTVLVEFFFARTFFHVTHFGDLASAFLLITIGTVSFASMGLVVASVTNTMQETQ
                     VINQLIWLPLIFLSGATVPLATLPQVVKRVGLFLPATYLVTGLQSAIYGAFAPWSLDV
                     LLALCSLAVWALLTFFLSAQLFRWEPESKIPRRAKLLAAATAIPFLLLGFWENKTNRT
                     MAQAQAMFDSLSAPSKSAPPTDSPK"
     gene            complement(8722..9642)
                     /locus_tag="VGR03_03290"
     CDS             complement(8722..9642)
                     /locus_tag="VGR03_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1993335.1"
                     /translation="MAIPILQVENLVKRYGDVEAVRGVSFAVEEGEVFGLLGPNGAGK
                     TTTVEILEGLRTPDGGRVSVCGLDPQRDSAALKHEIGAALQSTSLPDKLRVMEALRLF
                     ASFYKRRRDPEELLKRFGLEEKRNTFYNQLSGGQKQRLALAMALVNDPKVLFFDEPTA
                     GLDPQVRREIYNIIEELKREKKTIVMTTHYIEEAERLCDRVAIVDHGKVIALGSPRDL
                     KAHSGGTTRIEVRLSKPESNGTLKSLEGATDAREVDGAYVIHSMRPPQTIVSLVKHLE
                     AQGNELVSLEIASPSLEDVFIEMTGRRLRD"
     gene            complement(9745..10089)
                     /locus_tag="VGR03_03295"
     CDS             complement(9745..10089)
                     /locus_tag="VGR03_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEV1993336.1"
                     /translation="MSWDQVFRFVKQIPRGRVLTYGALAKALRLPGGARSAGRAMAAT
                     PSGKGIPWHRVLGERGKILIREPYASLQRKLLESEGIAIVESRVDLKRHLWKSPKKSA
                     LKHGAPRKRIQN"
BASE COUNT         2270 a         2805 c         3082 g         2148 t
ORIGIN      
        1 gtctccctgc ccgttctccc ggtgagagca ccattcccta attcctcttc ttcatccctt
       61 aagccctttg cttgccacac ttccaaggaa tcgcgcaaat cctcgacatt atcggacgca
      121 actgcgcctg caactccttt agattcaatc atcagttacg ctaactcctt tgctatccac
      181 acttccaaaa attcccacga aacttcaact ttaaatcctt ttgaatcaat accgctctct
      241 cctaagttct ttctttgcca cacatccgca aaatccaccc ataactcctt tctttgccac
      301 acatccaaaa acacaggact taaagtcctg tgtttgccac acatccgaaa aaagggggta
      361 gggtaggcaa attgttaact agaaacccca agaaggattt ctatctcagg cgaccatcgg
      421 gagcggaggg acctcccttc gtggcacagc cattcttggc tgttctctta cgaccggaga
      481 ctcattccgc gaggcacccg aacacggacc ggccgggcgc aagtcggccc ccctcgctct
      541 gccttattcc gaccctgcct ccggcacatc ttctcgtgtc agggccttca attcccgtcg
      601 caccacgtcc gccgaaaacc ccgcccgcag caggcttccg tacatggaag ccatcttctt
      661 ttcgtcgatt tcgccacgat acgagcgtaa cttccgttcg atacgctggc gcaccatcgc
      721 gccttcatcg gtttccttcg ccgcttcttc cagcgccgcc tcgatatgcc ggtccgggac
      781 gccgcgtgcc cgcaagtcgc gcgaaatccg aaacttcccc tgcttgcgcg tttccgtccg
      841 ctgccgggca aactgcttcg cgtatttcgc gtcgtcgatc tgtccatttt ccttcaaccg
      901 cgccatcacc agttgcacca gcagcttgtt atcgctccgc cgctccagct tccgcttcat
      961 ctcatgcacc gaatgcgccc gccgcatcaa cgcgcgcacc gcgacatcgt acaattctgc
     1021 ttcggtttcg agttggcgcg gcttcccgta catggctgaa gcatagaagc agggaagcac
     1081 ggatgcaagg aagtagagaa gtggcgagaa gtagaaatcg aaagaggagc ccgttccagg
     1141 aaattctttc tcctagcgtt caactcttaa ctttcgactt tgaactattt gcgggcgaag
     1201 cgctcgaagt cggccatttg cgaggccatt tcctcgcggg cggagtcggc ggcggagcga
     1261 ataccttgaa tgcgcagctt gaattcgtcg gggttcgacg cgcgcgccag ggcttcatcg
     1321 agcgtgatga gctgcttcga gtagaggtcg aagagcgatt gatcgaaagt ctgcatgccg
     1381 tactggctgg tgccggcggc gatggcatcg tgaatcaagc gcgttttgtc cgcgttgatg
     1441 atgcagtcgc ggatgtatcc cgtagcgatc atgatttcgc aagcgggcac gcggcctttg
     1501 tcatccgcgc ggcgcaccaa acgctgggag acgaccgcct tcaatgtggc ggcaagctgc
     1561 aaacgaattt gcttctgctc cggcggcggg aagaccgcga taatacgctg aatcgtttcc
     1621 gtggcgtcga gagtatgcaa agtggatagc accaggtggc cggtttccgc ggccagcagc
     1681 gcggtggaaa tggtttccag gtcgcgcatt tcgccgacca gaatcacgtc ggggtcctga
     1741 cgcagggcag cgcgcaacgc ggtggaaaag ttcgcggtgt ccacttcgac ttcgcgctga
     1801 ttgatgaaac tctttttgtc gcgatgcagg aactcgatgg ggtcttcgat ggtgatcaag
     1861 tggtcggcgc ggttagcatt gatgcggtcg atcatcgccg ccagcgtggt ggacttaccg
     1921 gaacccgtgg tccccgtcac cagcaccagc ccgcgctgct cttcgcaaat tttggagatt
     1981 acggggggaa ggttcaattc ttccgtggtg cgaattttcg tgggaatgac gcgcagcacc
     2041 ataccgacgt tgccgcgctg ctgaaaaacg ttgacgcgga agcggcccag gcccgcgacg
     2101 ccatacgcca tatccaattc cgctgtttcc ttgaattttt gcttctggcg gttggtcatc
     2161 atgctgaacg ccatcgagag catctcttcc ggcgtgacgc gaggcacgtc ggtgagcggg
     2221 gcaagcaggc cgtccacgcg caagtacggg tgattgccga ctttcaggtg caagtcggaa
     2281 gctttgcttt caacagcgcg acggagcaga tcatcaatgc ttacggccat gtcgtgtgaa
     2341 ccccgcaaga atgcagctaa tacgcgacac tagcatccgc gaaaagggcg ggcaagcaag
     2401 gtgagagccg ccgttgcgct ctagttaact gagcctcggg acaggttctt cgaggggaaa
     2461 tcgaatctca ggccttcttc aaatagagcg aatcgtagag caggccggcg acaaatcctc
     2521 cggccagcgg accgacccag tagactccct gatgcgccca gtgcgtcgct gcaagggcgg
     2581 gtccgaaggc acgcgcgggg ttcaatgccg cccctgttaa gggaccggca acaagaaagc
     2641 ccagcgaaat ggtcaggcca atgccgaagc ccgcgatcga gcgaaatgct cctttttcat
     2701 ctacggccgt cgcaaaaacc accagcacga ggaagaaggt ggttaccgcc tcaagggata
     2761 tcccggccca aaccggaaaa tctcttgcca gcgctggcgt acccaaagaa acggcgcgcc
     2821 aggtttcttc aggaacgaca gttttgagca gaaatgcagc ggccgacgcg ccggcaagct
     2881 gcgcaaccca aaagaggaag gcctcgatag tgccaaggcg cttggtgacc cagaagccga
     2941 tggtcaccgc gggattgaaa tgtccgccgg agatgtggcc gagcgcggaa accatgatgg
     3001 cgatggcgag accgtgtgcc agcgcgatgc ccaggagacc gaggccgccg cccgcgccgt
     3061 gcaaatattg atccgtgcag atcgagccca cgccaaaaaa aatcagggca aacgttccga
     3121 ggaattccgc gaccagtttt tgcgatgtgc tgtacatcgt tgatcctccg tgcaaggaaa
     3181 ggaaagcgcg cccgattcga acggctgcat tctatctgga atgcggcgcg gaacgccagc
     3241 cttcctcgcc aacggctaat caagcgaggg acgattgctc aattgtgctg gggcagtccc
     3301 acgcggcgca cgaggtcgga aaaccgcgga tcgctgcgca acggcatgag ggcttcatga
     3361 tcgagcagca tagtcatgcg cctatcgcgt cgctggaatg ccatctccaa cgcggcaaag
     3421 gcatgatcct tttcgcccag agctgcatag attccagcaa catcctgcca ggaagcgcga
     3481 tccgacttgg cttcctggat gacatcacca agaatctttc tagcctcctc gattctgccg
     3541 gtttgcgcgt atacataggc gagcatgctt cggtagacgg gcgattccgg cgaggactgc
     3601 acggcctgct caagctcctt cacagcctcc tcattttttc caccatgcac cagcgtaaga
     3661 ccaaaaagaa agcggccgag ggagaaattg cgctcaaggt cgagcgtttt ctggaactgc
     3721 gcgattgcct cattcgtccg cccgctgcgg gaaagaatct ggccatgtac cgcattaatg
     3781 atgagagaga agggatccgc atcctgcgcg cggcggatca aagccagcgc ttcgtcgtac
     3841 cgcgccagat tctccaagag ttccgcatac cactgcaatc cgatggcgta tccggggttg
     3901 atgaccagcg cccggcgaaa ctccctttcg gcgccggcaa aatcccagtc aaagattgcg
     3961 aggacctggc ccatggagac atgggtttcg gcaagcgaag gatccagttc cagagcctta
     4021 gttgccgcgg cacgcgattt ggcagcactt tcggcttgcg agatcgtgaa gtttttgaaa
     4081 aacgttacgt agtagcagtc cgcaaggccc gcgtaggcga gagcataatt gggatcccgt
     4141 tcaatggccc gctgaaaata gctcgtggcc ttgctcacgt cctcggcgtt tcgtttgttc
     4201 cagtaaaaac gtccgcggag atagagttga taggcttctc cattttcggt tgggcgctgc
     4261 gacaggcgcg atttttgtgc gccggttaaa ttcattttca ggttttcggt gatgtcctgc
     4321 gtgatctccg tctgtatgtt tggcacatcg gcgagcttcc agtggtattg cttgctccac
     4381 atgacgcggt catgctcggc atccacgaat tcggcgctga tggaaagatc gtcgccccgt
     4441 tgcaccatgc ggccggtaaa aatggtgtgg acgccaagtt cgcgggcaac cgacggcgga
     4501 tcgatgccct ttcctttata acgataagcc gaggccagcg aaacgacctt cagattggga
     4561 agctgcgaaa gggtgctgac aagatcctgg gtgattccat cgctcaggta ctcggaattc
     4621 gaatcgccgg tggcattcac gatcggcaag atggcaacag agtccatcga tttcagttga
     4681 atctcctgaa atccgtgccg ggcgactatg gcaatcccgg cgaggatcac taaggtgcct
     4741 gaaacgatgg ccaccttggc aagcagggac attcgcctac gtggcgcggt agcgggcggc
     4801 gcggaaggca cgcgcgcgga gtctgaatcg cgcttcaagc gtttgaggtc ggcgctgatt
     4861 tcggcggcgc tttggtagcg cagggtgcga tctttttcga gggctttgga gacgatttcg
     4921 tcgaagcgag aggggagatt cgaattcgcg gtgctcggat tcggcggtgt gcgctcgagt
     4981 atcgcgtgga agatgacggc cgatgtcggt cccgaaaaag cggcgcgtcc cgtggccatt
     5041 tcatatagga cggctccgaa gctgaaaaga tcggtccgtg catctaattc ttcgccgcgg
     5101 gcctgctcgg gagacatgta ggcaacggtt cccatcgcga ttccggggct ggttagggct
     5161 tcttcggttg ttacgccctc aagcgaaccg agcgtggttc ccggcgaact ggcgtcaggg
     5221 ttgagcttcg ccaaaccgaa atccatcagt ttcgcttggc cgcgcgtggt tatgaaaatg
     5281 ttcgcgggct tgatgtcacg gtgaacgatg ccctttttgt gcgcggcgtc gagtgcgtcg
     5341 gcgacttgaa taccgacgtc gaggagagca tccattgcga gaggacggcc ggtaacgcgg
     5401 gccttgagcg tttcgccctc gagcagttcc attacgagga aaaaccttcc ttcgtgttcc
     5461 tcaatgtcgt gaaaggtgca gatgttgggg tgattcagtg aggaagcggc gcgcgcctct
     5521 cggcggaagc gctccaaggc ttgaggattc ttgttgagtt cttccgggag gaatttgaga
     5581 gccacacggc ggccaagctt gaaatcttcg gcggcgtaga caatgcccat gccgccgccg
     5641 ccgagctttt caagtatgcg gtagtgggag agggtttggc cgatcatgag aaggattgcc
     5701 gctgcgcgaa atgatagggg gtggagagag gcgagtcaac gacggggaga attctctgag
     5761 aattgggagt caacagctag cttacaggaa tggggaaaaa gattaagaat taacacaaag
     5821 gacacggaga acacagaggg cgcagaggag aaataatgaa agacagaaac ttaccagatg
     5881 gtgggaatca gtttggagcg acggatttcg cggtccgccg taatcaggga gagtccttcg
     5941 acgagcgcgg tggctccgat tatgcgatcc gctggatctt tgggataagt tgctggaaat
     6001 gccatggcgc gcgcacacgc gcggccagtt atcgggaaga cgacaaagcg ggcttcgact
     6061 tcctggagga acgattcgag gctgattccg aggtggatgc ggcctttact cgcgagggtc
     6121 gtcaactcca ggagggtgat atcgcagatt gccaaaccgt ctccgttctt tcgtgaaatt
     6181 tcaattgact ccctggcttt ggccgagagc caggcaggat cggaggtcat ccaaagcaca
     6241 acatgcgtgt ctagcaaaat caccagagat ggccccaatc acgtgccaac agcgcagggg
     6301 agactacatc gccagtgatc gtgaccttcc cgcgaagaaa accgaaaata tcatcgactt
     6361 gtggcccgac cgggacgagc ttcgccacgg gcttgccgtg tttagtgatc accacggtca
     6421 cgcgcttgga ctgcacttcg tccatgactg caagacaatt tgccttgaag gcacctgctg
     6481 ccatcttctt catggtgaaa ctcctctaag actatggtca ttgtaccatg gtcatatttg
     6541 tgcagcaaac gccaagcgca ttccgactgc tttcgagcga ggatgcgggc ggggttttac
     6601 cggcggcgtt tttgtggaat tgttagcgaa cggtcgggct cggttgtagt ggcgcagcat
     6661 gctgcgccac tacaaagtca aagataaaga caattggaac gaactggggt tagcgagacg
     6721 ttgcgccgac gggggcgttt accggcgtga gccagttgtg gcgatcgggc ttgccgccgc
     6781 tggtgatggc gaagaactct tcctggagct tccgcgtgac cggtccggcc acgcccttgc
     6841 cgacctggat gtgatcgacc gagcggatgg gcgttacttc cacggccgtg cccacaaaga
     6901 aaacttcgtc cacaatgtaa agcatctcgc gcgggatgac cgtttccttc acggggatgt
     6961 tcagatcttc cgccagcttc acgatggcgt cgcgagtgat gccgggaaga atgctggtgg
     7021 ccagcggcgg agtgtagagc gtcccatcgt gaacgagaaa aacgttcatg cccgagcctt
     7081 cactgacgtg gccgccggaa tcgagcgcga tgccttcggt gtagccgttg aacgtcgctt
     7141 ccatgcgaat gagctgcgag ttcatgtagt tcgcggcggc cttggacatg gcgggaaggg
     7201 tgttcggggc gatacgggtc caggagctga cgccgacctc aacgcctttc gagagagctt
     7261 ccgggccaag ataggcgccc caactccagc aagccatgta aatgtcgatc ggcgaattca
     7321 gcggattcac tcccacttcg ccgtagccgc tcagcacgat gggctttacg taacaggaat
     7381 tcaacttgtt gagacgaacc agttcgcaag cggtgttgga gaattgatcg acggagaagg
     7441 gaagctccat gcggtaaatt ttcgcggagt tgatcaggcg ctgcatgtgc ttgcgcaacc
     7501 ggaagatggc cgggccctgt tttgtttcat aacaacggat gccttcgaag accgcggagc
     7561 cgtaactcac cacgtgcgaa aggacgtgta acttggcgtc gtcccagttg atccactttc
     7621 cgttgtgcca gattttctcg gtcgcctgaa tcggcataga tgctccctat tcgaaagtgc
     7681 cactcgccgt ggtaccgcgc gaaattgcag aatacggtac gcgagacaga gcgtcaatgc
     7741 gtcaaacgca attaaaactc agcgttcgga accaatttgg cgcgtggttg aacagcgcgc
     7801 cgggatccaa tgagccgcgc tactttggcg aatcggttgg cggggcagat ttcgagggtg
     7861 cactcagcga atcaaacatc gcttgcgcct gagccatggt ccggttggtc ttgttttccc
     7921 aaaatcccag aagaagaaac gggatagcgg tggcggcggc gaggagcttg gctctgcggg
     7981 gaattttgga ctcgggctcc cagcgaaaaa gctgggccga caaaaagaat gtgaggaggg
     8041 cccagacggc gagcgaacag agcgccagca aaacatccag actccaagga gcaaacgccc
     8101 cgtagatagc gctctggagg ccggtgacga ggtaggtagc ggggaggaag aggcctacgc
     8161 gcttgacgac ctggggaagc gtggcgagcg gcactgtcgc cccggaaagg aagatcaggg
     8221 ggagccagat gagctgattg ataacctgtg tttcctgcat ggtgttggtg acgctggcga
     8281 caacgagacc catagacgcg aaggacactg taccgatggt aatcaggaga aatgccgaag
     8341 cgaggtcgcc gaaatgcgtc acatgaaaga aggtgcgcgc gaaaaaaaat tcgacgagaa
     8401 ccgtgggtaa cgtgagcaca aaattagcta cgacgctgga agccagcata tcgctggggg
     8461 ttactggagt tacgtggaag cggcgcagga tgccctgctc ccggaacatg accagagcgg
     8521 cgctgagacc ccagaagctg cccatgacgg tgagggcgat gaccggaccg agaaaatatc
     8581 gaaccacgag ggggatgcct tttgcgaaga ctccggcgta gagaaagaag aaaccgaagg
     8641 gcatgattac gctgaaaaac aaaaacattt tattgcgcag cgtgagttga atgcgcatgc
     8701 gcgtcaaggt ggcgaagttc gtcaatcgcg caacctcctt ccggtcattt cgataaaaac
     8761 gtcttcgagg gagggcgaag cgatttccaa acttacaagt tcgttgccct gggcttcgag
     8821 atgcttgacc agggagacaa tggtttgcgg cgggcgcatg gagtgaatga cgtaggcgcc
     8881 gtcgacttcg cgcgcgtcgg ttgctccttc gagactcttc agggtgccgt tggactccgg
     8941 ctttgaaagg cggacttcga tgcgcgtggt gccgccggaa tgcgctttca ggtcccgcgg
     9001 tgaaccgaga gcgatcactt ttccgtgatc gacgatggcc acgcggtcgc acagacgctc
     9061 tgcttcctcg atatagtgcg tggtcattac aatcgttttt ttctcgcgct tcaattcttc
     9121 gatgatgttg tagatttcgc ggcgcacttg aggatcgagg ccggctgtgg gctcgtcgaa
     9181 aaacaagact ttgggatcgt tgacgagcgc catggcgagg gccaggcgtt gcttctggcc
     9241 accggagagc tggttgtaaa aggtgttgcg cttttcttca aggccgaagc gcttgagcaa
     9301 ttcttccgga tcgcgcctcc gcttgtaaaa gctagcgaag agacgcaaag cttccatcac
     9361 acggagcttg tccgggagtg aagtggattg cagcgcggcg ccgatctcgt gttttagggc
     9421 cgccgaatcg cgttgcgggt caagcccgca gacggatacg cgcccgccgt ccggagtgcg
     9481 aaggccttcc agaatttcca ccgtcgtggt cttgccggcg ccgtttggcc cgagcagccc
     9541 gaaaacctcg ccctcttcca ccgcaaagct gacgccgcga acggcttcga cgtcgccgta
     9601 gcgcttgacg agattctcca cttgaagaat gggaatagcc atgttcgttg cccctcacca
     9661 ctcaggggcc tagtcgaaaa ggagcgtata gcataccgca catagtcgtc tgcctagtaa
     9721 cgtgagaaca gatctgctgc ggcttcagtt ctgaatacgc ttcctggggg ctccgtgttt
     9781 cagtgcggat ttttttggcg atttccagag gtggcgtttc aggtccacgc gagattcaac
     9841 gatggcgatg ccttcgctct cgaggagttt tctttgcagg gaagcgtaag gctcgcggat
     9901 gaggattttg ccgcgctcac cgaggacgcg atgccaggga atgccttttc cagaaggcgt
     9961 cgcggccatg gcgcgtccag cgctgcgcgc gccgccgggg aggcgcaagg ctttagctaa
    10021 cgccccgtaa gttagaaccc ggccgcgggg aatttgtttc acgaagcgaa aaacctgatc
    10081 ccaagacatg tcgaatcaag ctccaaagag agatgagaag aaactcgtgc gacatcctag
    10141 gtgtcccttg ccggaagaac aagagaaaaa tggcgccgcc gggtttaatg caatagctgg
    10201 atgctgccga taattcaagt agcggcaggg agaacagcaa gcatctaatc aagtatcggg
    10261 cgacaaagtg gaaacggcgc gatgaaacga ggatgcgaaa catcg
//
LOCUS       DASYVO010000101         6791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_158861, whole genome shotgun sequence.
ACCESSION   DASYVO010000101 DASYVO010000000
VERSION     DASYVO010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158861"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..598)
                     /locus_tag="VGR03_03300"
     CDS             complement(203..598)
                     /locus_tag="VGR03_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEV1993337.1"
                     /translation="MQTLSVKTDRRTQLVDVTAQVQKGVASSGVATGICHLYVPHTTA
                     AITINECTDPDVARDVEGALNRLIPLAGPYRHSEGNSDSHVKAVLVGASQIVHVEGGK
                     LVLGRWQGVFFCEFDGPRDRRLQVKVVGD"
     gene            complement(805..2418)
                     /gene="bshC"
                     /locus_tag="VGR03_03305"
     CDS             complement(805..2418)
                     /gene="bshC"
                     /locus_tag="VGR03_03305"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HEV1993338.1"
                     /translation="MDCRALAFRQLPHQPKLFLEYLDHFEKVKSFYVHPPVMPAVTRV
                     ARKLDYPGERRAEVTSILRKQNIALGAGAETLSNLDRLDKGAVAVVSGQQVGLFSGPA
                     YSVYKALTAVQIAEELTRSGIPAVPVFWMATEDHDLDEVRHTTWFDQGRLVRFELPAA
                     AETGRPVGRIPLGAQIEPLVQQAAELLANQGSDLLAQYLIESYRPEETYGSAFGKLFA
                     RLFAQHGLILMDPLDAGLHKVAAPLYQHALVERDALNEKLLQRGKNLDRAGFDAQVKV
                     TSRSTLLFHLAGGVRQVITASAGKFQAGEKTWTRDELVHLTHNEPEKFSPNALFRPVV
                     QDYLLPTAAYIGGPAEISYIAQSEVVYRQLLGRMPVMLPRAGFTLVDAKANKLLRRYG
                     LTVEDVWAGSQGLRHKMERQSVPGSLSKNFDRNQKQIAKMLAQLGKQIEKLDRTLQGT
                     VERARKRIEYHIEKLRRKAGRAQDQKTGLVAAHEQHLESLLNPHKVLQERELCLLPFL
                     ARWGAGGLSELQKLSTGKKIGHHFIIQLP"
     gene            2530..4284
                     /locus_tag="VGR03_03310"
     CDS             2530..4284
                     /locus_tag="VGR03_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV1993339.1"
                     /translation="MPKPIGISLLWFILVQSATPLFAQVQAPQPASAQAPAVIRSTTR
                     LVQLNVIVHNKKGDPVQGLKKEDFTVLDQGQEQPVATFSANAAGPPDPSASATLPPNV
                     FSNRFDQSGQAPGSVTVILFDALNTEFLDQAYARQQVVKFLRQLKPQDHVAIYLLTTQ
                     LTILNEFTQDSSSLLRAIERFGGYSSAPLSASNPEPLNPRIPEASRSAPDAFASQLNA
                     FLDGASGRFSDFANINRAETTISAIEGIANHVAYVPGRKSLIWVSGSFPISIGYDADS
                     LFQIGREHRSFGDELEHAARALNQANMAIYPVDARGLMTSSQYSPSNNRRFDPVFSPG
                     SPPRFPRGGLVPDQNNFDTMTVLADRTGGKAFYNTNDIEGAVQRAIADGQFTYTLGFY
                     PTHGKWDGKFHELKVHVNEKGLTLRHRKGYFAAPDPPGGVSESQAALDAAVWSPVEWT
                     NLDLQVTLKAFQSSPRTIDLQIGLDTRELLLESKDGRWTGKVYVLLSQLGEGNKRISG
                     EQETFDLNLKPDTYEKFLKIGTKFTGRLTLSPDIVNLRVIAQDASSGSIGTLTIPIRK
                     LLQTASASSSKQPAGQKP"
     gene            complement(4306..5202)
                     /locus_tag="VGR03_03315"
     CDS             complement(4306..5202)
                     /locus_tag="VGR03_03315"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV1993340.1"
                     /translation="MILSGVFAALTTPYAHDGSVSLPDLKHNVHRYNQTDLAGYVALG
                     STGESVLLTRAEMDGILITVKEAAAQGKTLLAGAGAESTAETIERTKRAAELGYHAAL
                     VKTPFYYKPAYKPDVLIAHYRRVADESPIPVMLYSIPQFTGIALAPAEVAALAEHPNI
                     IGIKDSSGNVQGVAEIVAATPPAFQVLVGSAATVYPSLAIGARGAILALACALPEKCV
                     ALFELFRQGHHEKARELQSVLLRASKLIVSEMGIAGVKYVMDQRGYRGGIPRLPLQPL
                     SDERKRRIADLLAGLEPATARA"
     gene            complement(5206..>6791)
                     /gene="carB"
                     /locus_tag="VGR03_03320"
     CDS             complement(5206..>6791)
                     /gene="carB"
                     /locus_tag="VGR03_03320"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV1993341.1"
                     /translation="SGPNRIGQGIEFDYCCCHAAYALREDGFETIMVNCNPETVSTDY
                     DTSDRLYFEPLTLEDVLAIVEREKPQGVIVQFGGQTPLNLALELKRNGVPIIGTAPES
                     IDLAEDRRRFGRLLDELKIPQPRNATALVPEEAARVAGEIGLPVLVRPSYVLGGRAMV
                     IAYDVNTVQEYVAQAALMGPARPVLIDQFLEEATEIDVDALADGTDVVIGGIMEHIEE
                     AGVHSGDSSCVLPPVSLSPAILDTIREYTKRLALALKVVGLMNVQYAIQRDTVYVLEV
                     NPRASRTVPFVSKATGVPLAKIAARLMTGKKLASITLPVVQHNGVAEIAVHEFYSVKS
                     PVFPFNKFRGVDTILGPEMRSTGEVMGISTSYGLAFAKAQLAAGQRLPRKGTVFLSVN
                     DRDKKQLGALGKELSSLGFRLLATRGTAAALESAGIEAESVFKVNEGRPNIVDLVKTG
                     KIDLVINTPLGRESFYDEKSIRRAAIRYNIPCITTLAAAHAAARGIRALLEQGTEVAA
                     LQDLHRGQAAASGVPASGD"
BASE COUNT         1425 a         2107 c         1819 g         1440 t
ORIGIN      
        1 agcaggagtc cgatctcggc catcgtccct cctgcttgat cagaaaacac gctccccctc
       61 cgcttgtcga cccctcaaaa cgcacactgt gccgagcatg tcaacagagc cactttaact
      121 ccctttagaa tcaacactta tgaaaaacag ggggggttgg ggggggttac ccaatcacta
      181 aaattttgct tgaattccgg tctcaatcac ccacaacctt cacctgcagc cgccggtcgc
      241 ggggaccatc aaactcgcag aagaacacgc cttgccagcg ccccagcacc agcttgccgc
      301 cttccacgtg gacgatttga ctagctccca caagcacggc tttcacatgg gagtctgagt
      361 tgccctcgct atgccgatac ggccccgcca gggggatcag ccggttcagc gcgccctcga
      421 catctcgggc gacgtccggg tccgtgcact cgttgatggt aatcgccgcc gtggtgtgcg
      481 gcacatataa atggcaaata cccgtggcga caccagacga cgcgacaccc ttttgcactt
      541 gcgccgtcac atccaccagc tgcgtgcgcc gatccgtttt cacgctcaat gtctgcatgg
      601 aattccctcc ccgtccggcg ccgggattct agcacgccct catacgctac ccagcggtgc
      661 cgctttattg cccctgtagg gacgcagccg tgctgcgccc ttgcttggca agacctcagc
      721 ggaagctcat tccgagcgca gcgaggaatc tgcttctaat tgtttgcacg caaggaagtg
      781 ttgatcgttt ctatattcag gacgctacgg aagctgaata atgaagtgat gcccgatttt
      841 ctttcccgtt gaaagtttct gcaattcgct gagcccgccg gctccccaac gcgctaggaa
      901 cggcagcaag cacaattccc gctcctgcaa taccttgtgc ggattcagca gcgactccag
      961 atgctgttca tgcgccgcga ccagcccggt cttttggtcc tgcgcccggc ctgctttgcg
     1021 ccgcagtttt tcaatgtgat attcgattct cttccgcgct ctttcaaccg taccctgcaa
     1081 cgtgcggtcg agcttctcga tctgcttgcc gagctgcgcc aacatcttgg cgatctgttt
     1141 ctggttgcgg tcaaagtttt tcgacagaga gccggggacc gattgccgct ccattttgtg
     1201 ccgcaagcct tgcgatcccg cccaaacatc ctcaaccgtc aggccatacc ggcgtagaag
     1261 tttattggcc ttcgcgtcga ccagcgtgaa tcccgcgcgc ggcagcatca ccggcatcct
     1321 tccgagaagc tgccggtaca ccacttccga ctgcgcaata taagaaatct ccgccggacc
     1381 gccaatatac gctgcggtgg gaagcagata gtcctgcacg acgggccgga agagcgcatt
     1441 agggctgaat ttctccggct cgttgtgcgt caggtgcacc agttcgtccc tcgtccacgt
     1501 tttctcgcca gcctgaaatt ttcccgcgct cgccgtgatg acttgccgca cgccaccggc
     1561 caaatggaac aacaatgtac ttcgcgaagt caccttgacc tgggcatcaa atcccgcacg
     1621 gtccaaattt ttcccacgct gcagaagctt ttcgttcagc gcgtcgcgtt ccacaagcgc
     1681 gtgctgataa agtggggcgg cgactttatg caagccggca tcgagcgggt ccatcaggat
     1741 caacccgtgc tgcgcaaaaa ggcgggcaaa tagtttgccg aacgcgctcc cgtacgtctc
     1801 ctctggacgg tagctttcga tcagatattg cgcaagcaga tcgctccctt gattcgcaag
     1861 cagttctgcg gcttgctgca ccagcggctc gatctgtgcg cccagcggaa ttcgtcccac
     1921 cggccgtccc gtttccgcgg cagcaggcaa ttcaaaacga accagtctcc cctgatcgaa
     1981 ccaggttgtg tggcgaacct catcgagatc gtgatcttcg gtggccatcc aaaagaccgg
     2041 cactgccgga attccgctcc gcgtcaattc ctcggcgatc tgaacagccg tcagcgcctt
     2101 ataaacggaa taagccggcc cgctgaacaa tcccacttgc tgcccggaaa cgaccgcgac
     2161 ggcacccttg tcaagccggt ccaaattgga tagcgtttcc gctcccgccc cgagagcgat
     2221 gttctgtttg cgaagaatgg aagttacttc tgcccgccgc tcgccgggat agtccaactt
     2281 ccgagctacc cgcgtcaccg ccggcatgac cggcggatga acgtaaaatg acttcacctt
     2341 ctcaaagtga tccaggtatt caagaaagag ctttggttga tggggaagct gccggaaggc
     2401 aagagcacga caatccatga gatgttagat gatactcgcg gcaaaacgtc tcatcaaatc
     2461 tgagggtagg gcttgtgact gccgcgttcg tatgttagtt ttgcagatat tcatcttcta
     2521 aaggtaccca tgccgaaacc aattggtata tcccttctgt ggtttatttt ggtccaaagc
     2581 gccacgccgc tctttgcgca agtccaagct ccgcaaccgg ccagcgctca agcgcccgcc
     2641 gttatccggt caacaacaag gctggttcag ctcaacgtca ttgtacacaa taaaaaaggc
     2701 gatcccgtcc aaggcctgaa gaaagaagat tttacggtcc tggaccaggg acaagaacaa
     2761 ccggtcgcca ccttctccgc caacgccgcg ggaccgccag atccctccgc aagcgcgact
     2821 cttcccccga acgtattctc caaccgcttc gaccaaagcg ggcaggcccc gggaagcgtg
     2881 actgttatcc tcttcgacgc gctgaatacg gaatttctgg accaggccta cgcccgccaa
     2941 caggtggtta aattcctccg tcagctcaag ccacaggatc acgtggcaat ctatctgctg
     3001 accacgcaac tcaccatcct gaacgaattt actcaggatt ccagttcttt gttgcgcgcc
     3061 atcgagcgat tcggaggcta ctcttcggcg ccactcagtg cttccaaccc cgagcccctc
     3121 aatccgagga tacctgaggc aagcagatcg gctccagatg cgttcgcttc ccaattgaat
     3181 gcatttctgg atggggcgag cggaagattc agcgattttg ccaatatcaa ccgagccgaa
     3241 acgacgatca gcgccatcga aggcattgca aaccacgtgg cctacgtacc cggcaggaag
     3301 agccttatct gggtctccgg cagcttcccg atcagcatcg ggtatgatgc agattccctg
     3361 ttccaaatag gtcgcgagca tcgctccttc ggtgacgaac tcgaacatgc agcgcgcgcc
     3421 ttgaaccaag ccaacatggc gatttacccc gttgatgcgc gcggcttgat gacgtcctcg
     3481 caatacagcc cgagcaataa ccgcaggttt gatcccgtgt tttcccccgg gtctccgccc
     3541 agatttccgc gcggaggact tgtgcctgac cagaataatt tcgataccat gaccgttctc
     3601 gccgaccgca ctggaggaaa ggctttttac aacaccaatg acattgaagg ggccgtgcag
     3661 cgtgcgatcg ccgacggcca attcacctac acccttggct tttatccaac ccatgggaaa
     3721 tgggatggca agtttcatga gctgaaggtg cacgtcaatg aaaaggggct gacgcttcgc
     3781 catcgtaaag ggtatttcgc tgcacctgat ccgcccggcg gagtctccga aagccaagcc
     3841 gctctcgacg ctgcggtctg gagccccgtc gaatggacga atctcgatct gcaggtgacc
     3901 ttgaaggcgt ttcaatcctc gcctcggacc attgaccttc aaatcggatt ggatacgcgc
     3961 gaactcttgc tggagtcaaa ggatggccgc tggactggaa aggtgtacgt actccttagc
     4021 cagttggggg agggtaacaa gcgcattagc ggtgagcagg aaacgtttga tctaaacctg
     4081 aagccggaca cttacgagaa gtttttaaag ataggtacta aattcacagg ccggctgacg
     4141 ctgtctccgg atatcgtgaa tctccgcgtc atcgcgcaag atgcttcatc gggttccatc
     4201 ggaactttga ccattccgat caggaaactc ctccaaacag ccagcgcatc cagcagcaaa
     4261 cagcccgcag gtcaaaaacc atagacagga aaaaatgtac tgaggtcagg cacgagccgt
     4321 ggccggttcg aggccggcga gcaagtctgc tatccgcctt ttccgttcat cggagagggg
     4381 ctgtagcggg agccgcggaa tgccgccgcg gtatccacgc tgatccatga cgtacttcac
     4441 gcccgcaata cccatctcgg aaacgattag ttttgaggcg cggagcagga cggattgcag
     4501 ctcgcgcgct ttttcgtggt gaccttgccg gaacaattcg aagagcgcca cacacttttc
     4561 cggaagtgca catgccaacg ccagaatcgc tccccgcgcg ccaatcgcca gcgacgggta
     4621 caccgttgcc gcggagccga ccaagacctg aaacgcaggc ggggttgccg ccacaatttc
     4681 cgcgactccc tgcacatttc ctgaactgtc tttgatgccg atgatgttgg gatgctcggc
     4741 cagcgcggcc acttctgcgg gcgccagcgc gatgccggtg aattgcggaa tcgagtagag
     4801 catgactgga atcggagatt cgtcggcgac tcgccggtaa tgcgcgataa gcacatcggg
     4861 tttgtacgca ggtttgtagt agaacggagt cttcaccagc gccgcgtggt atcccagttc
     4921 tgccgcgcgc ttggtgcgct caattgtttc cgcggtcgac tccgcaccgg cgccggcgag
     4981 cagcgttttc ccttgcgccg ccgcttcctt taccgtaatc agaatcccat ccatctccgc
     5041 tcgcgtgagc agcacagatt ctcccgttga acccagcgct acgtagcctg ccagatcggt
     5101 ctggttgtac cggtgaacgt tgtgcttcag gtcgggaaga gaaaccgatc cgtcgtgcgc
     5161 atacggcgtg gttagagcgg cgaaaactcc ggacaatatc acggcctaat ctcctgacgc
     5221 aggcacgccg gaagcggcgg cctgtccgcg gtgcaaatcc tgaagcgcgg caacttccgt
     5281 tccctgctcg agaagcgcgc gaattccgcg cgcggcggca tgcgcggcgg ccagcgtggt
     5341 aatgcacggt atgttgtagc ggatcgccgc gcgccgaatc gatttctcgt cgtagaacga
     5401 ttcgcggccc agtggagtgt taatcaccag atcgatcttg cctgttttca ccaggtccac
     5461 aatgtttggc cgtccttcgt ttactttgaa aaccgattcg gcctcgatgc cggcggattc
     5521 caacgcagcg gcagttccac gcgtcgcgag aaggcgaaag cccagcgacg aaagctcttt
     5581 tccaagggct cccaactgtt ttttgtcgcg gtcgttgacg ctcaggaaaa cggtgccctt
     5641 gcgcggtaag cgctgccccg cagcaagctg cgccttagcg aacgctagtc cgtacgatgt
     5701 cgaaatcccc atgacttcgc ccgtcgagcg catctcgggg ccgaggatgg tatccacgcc
     5761 tcggaacttg ttgaagggaa acaccggcga ttttaccgaa tagaattcgt gcacggcaat
     5821 ttccgccacg ccgttatgct gcaccacggg cagcgtgatg gacgccagtt tctttccggt
     5881 catcaaacgc gccgcgatct tcgccagcgg cacccctgtc gctttgctca cgaaggggac
     5941 ggtgcgcgag gcgcgcggat ttacttccaa aacgtatacc gtgtcacgct gaatcgcgta
     6001 ctgcacgttc atcaatccaa ccactttcaa tgccagcgcc agccgtttcg tatattcacg
     6061 gatggtgtcg aggatcgcgg gtgacaaact cacgggcggc agaacgcatg acgaatcccc
     6121 cgagtgcact ccggcttctt cgatgtgctc catgattccg ccgatgacca cgtccgttcc
     6181 atcggccaga gcgtccacgt cgatttccgt cgcttcctcg agaaattgat cgatcagcac
     6241 gggccgcgcg ggccccatca gcgccgcttg cgcgacgtat tcctgcacgg tgttcacgtc
     6301 atacgcgatg accatcgcgc ggccgccaag cacatagctc ggccggacca gcaccggcaa
     6361 tccgatttct cccgccacgc gagccgcttc ttccggcacc agcgcggtcg cgttgcgcgg
     6421 ctgcgggatc ttcagctcgt cgagcaaacg gccgaaccgt cggcggtctt ccgcaagatc
     6481 gattgactcg ggagcggtgc caatgattgg tacaccgttg cgcttcagtt ccagcgcaag
     6541 attcagcggg gtttgaccgc cgaactgcac gatcactccc tgtggtttct cgcgttccac
     6601 gatggccagc acatcttcca gtgtgagcgg ttcgaaatag aggcggtcgg aagtgtcgta
     6661 gtccgtcgat accgtctccg gattgcaatt caccataatg gtttcgaaac cgtcttcacg
     6721 cagcgcgtag gcggcatggc agcagcaata atcgaactcg attccctgcc caatgcgatt
     6781 cggaccgctg c
//
LOCUS       DASYVO010000102         4695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_87406, whole genome shotgun sequence.
ACCESSION   DASYVO010000102 DASYVO010000000
VERSION     DASYVO010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87406"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /gene="lptC"
                     /locus_tag="VGR03_03325"
     CDS             complement(<1..826)
                     /gene="lptC"
                     /locus_tag="VGR03_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEV1993342.1"
                     /translation="MRSSQATRYARWSALTALLIVVMVTGMYARRAWQLAHALKKAPP
                     AVSVSVTQRSATFAFSKVEGERTLFTVRASHATEFKEGNKNLLEDVWITVYGRTGARF
                     DNLHTQQCDYLRDTGRVVCKGEVQIDLESAEEAKQPAAQRGIRVVTSNLSFDRQTGEA
                     QSDQPVTFRFPYGQGRAVGVNYSSNDAAVRLLSDVQMTLNQALSKSAAEPIELSGGSM
                     EYRRESRVMRLAGPVRVHQGARELTAGLITLELSPSLRARRLTATEKPQMISREPQG"
     gene            1153..2574
                     /locus_tag="VGR03_03330"
                     /pseudo
     CDS             1153..2574
                     /locus_tag="VGR03_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
     gene            complement(2884..4041)
                     /locus_tag="VGR03_03335"
     CDS             complement(2884..4041)
                     /locus_tag="VGR03_03335"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEV1993343.1"
                     /translation="MVEENKNFDGVERRRNPIVPHVKADPRGLSEVAVFHELGKALTS
                     SLQLDQVLRTIMQKVEEFLHPDTWSLLLMDEAKQELYFEIATGEASAALKDIRIKLGQ
                     GIAGWVAQTGEVVIVPDTSKDSRFFSQVDEKTKMETRSIVAVPVRFRDRALGVIELIN
                     CVGPDGFGERDLALLEVLADFAAIAMENARHVQRIHELTITDDCTTLYNARHLNFVLE
                     TEIYRSQRYNYEFSLIFIDLDHFKNINDTYGHLTGTRLLADIGNMVKAGCRLIDFAFR
                     YGGDEFVVLLPQTDKENAFVVARRLHKRIRESTWLEETGLNVRITASIGVASYPSDSR
                     DKSKLLSMADEAMYLVKNTTRDSVAAAGMGVIPSLEAPVEPPRAPARRSDS"
     gene            4416..>4695
                     /locus_tag="VGR03_03340"
     CDS             4416..>4695
                     /locus_tag="VGR03_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1993344.1"
                     /translation="MEKTTFGEHLRREREMRGVSLEEISAATRIGVRFLEALENEQWD
                     RLPGGVFNRGFIRAVARFLGLDEEGLVGEYALLTHDKPEKAVWNTAAIS"
BASE COUNT          950 a         1348 c         1449 g          948 t
ORIGIN      
        1 gcccttgcgg ctcgcgggag atcatctgcg gtttttcggt ggcggtcagg cggcgcgcgc
       61 gcagagacgg cgagagctcc agcgtgatca ggccggcggt cagctcgcgc gcgccctggt
      121 ggacgcggac gggacccgcc aggcgcatca cgcgactctc gcggcggtac tccatgctgc
      181 cgccggatag ctcgatgggt tccgccgcgc tcttggaaag ggcctgattg agagtcatct
      241 gcacgtcgct gagcaggcgc accgccgcgt cgttgctgct gtagttgacg cccacggcgc
      301 ggccctggcc gtagggaaag cggaacgtga ccggctgatc gctctgcgct tcgccggtct
      361 ggcggtcgaa cgatagattg gaagtcacca cgcggatgcc gcgctgggcc gcgggctgct
      421 tggcctcctc cgcggattcc agatcgatct gcacttcgcc cttgcagacc acgcggccgg
      481 tgtcgcgcag gtaatcgcac tgttgggtgt ggaggttgtc gaagcgggcg ccggtgcggc
      541 cgtagacggt gatccacacg tcctccagca ggttcttgtt tccctccttg aactccgtgg
      601 cgtgggaggc gcggaccgtg aacagcgtgc gttcgccctc cacctttgaa aaggcaaacg
      661 tggcggagcg ctgcgtgacc gagaccgaca ccgccggcgg cgccttcttc agcgcgtggg
      721 ccagttgcca ggcgcgccgc gcgtacatcc ccgtgaccat gacgacgatc agcagcgcag
      781 tgagcgcgga ccagcgggca tagcgagtgg cttggctgct acgcattaga aaatgtacct
      841 gctacctgtc cgaactccca gtataggcat tcggttgcct gcggccaaat caacggaggg
      901 ataggagaca ggacgattca aaattgtagc ccgcttgcgc tggtctaccg ggacgcggtt
      961 cggcacaccc atatttctat gccacccccg caggggcccc agcccccaga cgcgtcgctg
     1021 gatcaaggag cgcaatcaca actgacgtcg tgggggcgtt caaaaccgca ctcccgagag
     1081 acgtttccgc ccttcacagc tatactaaac ttggcgccct tgactgtctg gattggcggg
     1141 gaacactaaa caatgccacg aatcacagcc caggacttct atgattacga caagtgccca
     1201 catcgggtgt acatgaacta tcatggaaat cctgatgaaa agcttcctct ctccgatttc
     1261 ctgaatctgc tattcgaaac tgccttgctc cacgagcaaa gaatcattca tgggctgccg
     1321 ttccaatccc ctgttggaga aacaatagag gcgcaggccg agtcaacaat cagtttgatg
     1381 ggcgatggcg tggaccgaat ttaccagggg gtacttctgc tggcggatga cagcgggagc
     1441 ccagacttgc tcgaaagggt tccgggaaat tccagatttg gtaattattt ctacaagcct
     1501 gtcgacataa agagcggcag cggtttcaga gacgaagaca aaggaacact cagagaagac
     1561 tacgctctcc agctgtgtca ctacgctgaa ctccttaaga aaattcaggg cacttttccg
     1621 ccacaggggg agattctcaa taagagaggc caacgagtcc cctataacct cgccgcgtac
     1681 gccactgatt atgcggccgt gatgccagag gtcagggcac tcgtcgaagg aacaaggagc
     1741 gatgagcctg tgctgagcag ccactgcggg aaatgccagt ggttcggtca ttgcgagccg
     1801 atacttgaaa aggctaacga cgtttctctt ctgcctgaca tcggtagatc taagaagatg
     1861 aatctcaagc aggccggtgt tgtatcaatt caggatgttt ctaattttga cttcaggacg
     1921 aggaaagttc ctggcttcgg tcctaagacg gtcgattcaa ttaagcggaa cgcacgagtc
     1981 gtgctttccg gaaagatgga attaattcag agaccaaatc tgcctaaccc ggctttgaag
     2041 gtctatttcg acttcgagga tgatcccact caagacctca tttacttggc tgggatgttg
     2101 tttgagccac cgagagaggg gacagagttt cgatacatat tcgggccgga tgaaggcggc
     2161 gaaggggtcc tctggcggga cttccaagcc ttgtgcgtat caattgccaa agaggatttt
     2221 cttgtctttc actactcgtc ctacgagaag tcgaagatca cccagctgga gaacaaatac
     2281 ggcctggctg agagggctgc ggtcgagaat ttccggagca aaatgattga ccttcattcc
     2341 gaagtcgaac acagcctagc gctcccaaca cgcagctagg gactcaaggt agtcgcgccc
     2401 tttgccggcc acaggtggag cgcggaagat gctggcggag cccagtctat tgtctggttc
     2461 caagagtacc aggctgaccc gacgaagaga gaagtcttgg caagactgct tgaatacaac
     2521 gaagaagatt gccgggctct taaggcaatc tatgaatggt tgaaaacgct ctaaattacg
     2581 ccctgccgag cgtccggtgc cccatgttcc ggttttgaac gtggggactt ctcttaaacc
     2641 gacgatccgt tgagcggtgt gcgcgaatgc gacgttcacg cgccggcgtc ctcggcttga
     2701 ctctcgcgtc gggctgggtc cgcctgacgt aattgaaccc caacgtgata gcgcccagcg
     2761 tgattagaga agactcgata attctcttct gactccgccg ttcgtgcgaa gcaagtggga
     2821 tcaccatgct tgcacttact cctctgccgt aatacccgtg ggattccctg tggaagggcg
     2881 acgctaagaa tcgctgcggc gggcgggcgc gcgcggcggt tcgacggggg cctcgaggct
     2941 cgggatgacg cccatgcccg ccgcggccac gctgtcgcgc gtggtgttct ttaccaggta
     3001 catcgcttcg tcggccatgc tgagcagctt cgacttgtcg cgcgagtcgg agggatagga
     3061 ggccacgccg atgctggcgg tgatgcgcac gttgaggccg gtctcctcga gccaggtgga
     3121 ttcgcgaatg cgcttgtgca gccggcgcgc caccacgaag gcattctcct tgtccgtctg
     3181 cggcagcagg acgacgaact cgtccccgcc atagcgaaat gcgaaatcga tcagccggca
     3241 accggctttg accatattgc cgatgtcggc cagtagccgc gtgccggtca ggtggccgta
     3301 ggtgtcgtta atgttcttga agtggtcgag gtcgatgaag atcagcgaga actcgtagtt
     3361 gtagcgctgc gagcggtaga tctccgtctc caagacaaaa ttcaggtgcc gcgcgttata
     3421 aagcgtggtg cagtcgtcgg tgatggtgag ctcatggatg cgctgcacgt gccgcgcgtt
     3481 ttccatggcg atggcggcga agtcggccag cacctcgagc agcgccaggt cgcgctcgcc
     3541 gaagccgtcc gggcccacgc agttgatcag ctcgatcacg cccagggcgc ggtcgcggaa
     3601 gcgcaccgga acagcgacga tcgagcgcgt ctccatcttg gttttctcgt ccacctggga
     3661 gaagaatcgc gaatccttgc tggtgtcggg gacgatcacc acctcgccgg tctgcgccac
     3721 ccagccggca attccctgcc ccagcttgat gcgaatatct ttgagcgccg cgctggcctc
     3781 gccggtggcg atctcgaagt acagctcctg cttggcttca tccatcagca gcaaggacca
     3841 ggtgtccggg tgcaggaatt cttcgacttt ctgcatgatg gtgcgcagaa cctgatcgag
     3901 ctggagagaa gaagtgaggg ccttgccgag ttcgtggaat accgcgactt cgctcaagcc
     3961 gcgggggtcc gccttgacgt gggggacgat gggattgcgc cggcgctcga cgccatcgaa
     4021 gtttttgttt tcctccacca ttcggctgaa tccaagagct gctggtctag acatctccgt
     4081 agccatggct gcccgggttc gcaccggcgg cggggaaccg gcaacccatc ccggctcgga
     4141 ttatagggaa cgcccttccc gatgtgcaac ttccgtgttg agcggctgtg cccagtgggt
     4201 tccggggcac tctgcaagcc tgtcccgggc catatcccat tttggggcgg gaacggaaac
     4261 gacgcagccc gccaattaag aaacgagaag aatcgccgct gagggcagct cgtcggcagc
     4321 acggattgac agcgcgagcg aggctgccta tagtgccaat tggaattggg tgctcgtgct
     4381 gcggcgcccg gaaccacgca gccgctccct cccccatgga aaaaaccacc ttcggcgagc
     4441 atctgcggcg cgaacgcgag atgcgcggcg tctcgctgga agaaatttct gctgccaccc
     4501 gcatcggcgt ccgtttcctc gaagcgttgg aaaatgagca gtgggatcgt ctgcccggcg
     4561 gcgtgttcaa tcgcggattt atccgcgcgg tggcccgctt cctcggtctg gatgaagaag
     4621 ggctggtggg cgagtacgcg ctgctgacgc acgacaagcc cgaaaaggcc gtgtggaaca
     4681 cggcggcaat ctcgg
//
LOCUS       DASYVO010000103        20335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_87421, whole genome shotgun sequence.
ACCESSION   DASYVO010000103 DASYVO010000000
VERSION     DASYVO010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 20335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20335
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87421"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VGR03_03345"
     CDS             complement(<1..202)
                     /locus_tag="VGR03_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993345.1"
                     /translation="MDISVIAFLLLLFAVAALRLGELRISRRHQERLVSRGAAKVDEP
                     RFRWMVALHAAVLIGAALEVLLL"
     gene            complement(193..1287)
                     /locus_tag="VGR03_03350"
     CDS             complement(193..1287)
                     /locus_tag="VGR03_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEV1993346.1"
                     /translation="MESASNRPLETAAASYPTIAATATAVPPHTITRDDVKYYMGRVF
                     DIPDRKLEAMMTVVDNAQVHKRHSIFPIEYTIEPRPLSKTNEEYQEHAIALGRRAAEE
                     CLAKAGVAPADVDLIITVSCTGYMVPSLDVHLLNLMGFRNNIRRLPITELGCIAGAIA
                     LSRAADFLRGAPGKTVLIIAVELPTLTFQRKDLSQANLISAVLFGDGAAAAVITNRPS
                     SGPRILDSESFTLPGSMDALGFDLRDTGFHIVLSKDVPQLLRDKIKGLVENFLGRHNL
                     TPQEMTAYLLHPGGQKLLICIEEELGLRRCHTEFSWDVLRDYGNISSATILFILQEWL
                     TKKKMKPGDYGLMAGFGPGFSMELLLLQWT"
     gene            1632..2726
                     /locus_tag="VGR03_03355"
     CDS             1632..2726
                     /locus_tag="VGR03_03355"
                     /inference="COORDINATES: protein motif:HMM:NF005071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV1993347.1"
                     /translation="MRGTAFRHTVLGAVGLLLVSLPVRGQVSQWRGATEGDFVAKKFK
                     FRSGEMLPELRLHYTTLGKPARNAEGRVTNAVLILHGTGGTGQQFLQPQFAGELFGPG
                     QLLDVTRYFIIFPDGVGHGKSSKPSDGLHAHFPQYDYDDMVAAHHQLLSEGLGVNHLR
                     LILGTSMGCMHSFVWGETYPDFMDALMPLACQPVQIAGRNRVWRKMVMDAIRNDPEWK
                     GGEYSAEPQQGLRTALDLLLIAGSAPLYMQKNLPTRDTADKYLDDYFKTRFAGLDAND
                     LLYQVNASRNYDPSPQLEKITAHVMYINSVDDFINPPELGMAEREIKRVKNARFILIP
                     ISDETRGHGTHTRAVVWKQYLVELLEKSAH"
     gene            complement(2749..4581)
                     /gene="typA"
                     /locus_tag="VGR03_03360"
     CDS             complement(2749..4581)
                     /gene="typA"
                     /locus_tag="VGR03_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV1993348.1"
                     /translation="MRQNIRNIAIIAHVDHGKTTLVDAMLRQSGIFRANEALIERVMD
                     SNDLERERGITILAKNTALFFHDTKINIVDTPGHSDFGGEVERALRMVDGVVVLVDAS
                     EGPLPQTRYVLQKALAAKLPPVIVLNKIDRTDARAKEVLDEIYDLFIDLDATEDQLDF
                     PVVYTNARDGVAHMTPGDDSKDLLPLFETIVKHIPAPPGDPEGVLQIQVMNLDYSEFL
                     GRIAIGRVFNGTLRRGADVGIVKLDGRLTPTRITKLYTFRGLERDEAQAVPAGDLVAI
                     AGVEGINIGESITDLANPMPLEPLLIDEPTLAMIFTVNSGPLSGKDGTFLTSRDLRDR
                     LQKELLTNVSIRVEDTDTPESFRVLGRGELQLAILIETMRREGFELMVGKPEIVVRTE
                     NGKKLEPLERLVIDVPENFIGIIMETIGSRRGEMIKMSNHGSGRVRMDFKIPSRGLIG
                     LRSQLLTDTRGTALIHSIFEGWMEYGGEMALRPTGALVADRGGPSTAFALWNIQERGE
                     LFVGPAIDVYEGMIVGENSREQDMDVNVTKEKKQTNMRSSSADEAIRLIPHRQLSLEQ
                     AIEFIADDEYVEITPKSIRLRKKVLQANKRPRRWQEIRASQATT"
     gene            complement(5247..7952)
                     /locus_tag="VGR03_03365"
     CDS             complement(5247..7952)
                     /locus_tag="VGR03_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1993349.1"
                     /translation="MAPRKASGPKIGAVVAPQEDIMAAADSRATRIYRALLRLLPFDF
                     RSDFGPEMETVFKEQHKEAGRRNGATGVLRLWWETIVGIFRTASGEHLTMFRQDAGFA
                     LRMMRKSPGFTLAAILTLGLGVGANSAIFSVVNAVLLKPLPYEHGDRLVTLHQQAPRA
                     GMLNQPFSVAEINDYRTQSHSLDGLVEYHNMNFILLGRSEPERVETGVVSWNYFDVFG
                     VKPLFGRAFRPEDEQPGAPAVLMLSYEYWIRSFGGDPTVVNKSFTMNDRLHTVVGILP
                     PVPQYPDENDVYMPTTACPFRSRPAFIANRQSRMMQAFGRMKPGIRLSQAQADLNGVA
                     ANLQKAYPNDYPPSIDYSLGTSSLAEELTHNARPTMLVLLGAAGFVLLIACANVANLN
                     LSRMVRRERELAVRAALGAGRARMFRQLLTESSLLALLGGGLGLLFSWGALNLLVSFA
                     ARFTPRAREIHLDATVLLFTLAVAVLTSLLSGTAPALAARETVVGSLKEGGAQSTIGR
                     GRHQMRSLLIVAQVAVSFLLLIGAGLMLRSFAKLQHVDPGFQPENVLTMQIGLDFTKY
                     GTNDKQRAFFETFLDKIEAQSGVKSAAASMMIPLNGNMRMTGDFQIEGQTPVPGQALP
                     VSDFRVVSERYFETLHIPVLAGRAFMRADRPGALDVAIVNRAAARHFWGTQDPVGKRF
                     SADGGKTWVQIAGVVGDVKQYGLDKAPVDEIYVPMAQNPLMDAVLVVKTTAEPMSIAR
                     SVIELLYAVDPNQPAARVRSLEQVRAESVAAPRLTTSLLGLFALLALAIAATGIGGVM
                     ALAVGQRRHEIGVRMAIGARPGEILRMILGQGMTLALVGVALGLFGALWLTRLLEQLL
                     FEVAPTDPLTYVGVALVLGLAALVACYVPARRAARVDPIIALRVE"
     gene            complement(7976..8344)
                     /locus_tag="VGR03_03370"
     CDS             complement(7976..8344)
                     /locus_tag="VGR03_03370"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1993350.1"
                     /translation="MTAAQSHAEPFGPLTPSMFHVLLALAGDDLHGYAILKEVELRTG
                     GKVRLSTGTLYGIIKRLLNDGLIVELRARPAEAEDDERRRYYRLTPQGRQVATAEAER
                     MDEILSIARSRNLLKKTRPA"
     gene            complement(8447..9382)
                     /locus_tag="VGR03_03375"
     CDS             complement(8447..9382)
                     /locus_tag="VGR03_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1993351.1"
                     /translation="MRRFMTLYAANALLLLSVAAMALGRPPQASSITQVATEAASAYD
                     AKNWSESAKLYGQIVQTAPNPRAWYRLGVSLNKTGEKEKAIEAFEKGRALGLPPQFAD
                     YGIATVFVSKKDKDKAFEHLEKAAQNGLGQPDQLSADPDLAELKSDPRFAKILELVTR
                     NQKPCAYAAENRQFDFWLGEWSVVTSQGETLAGDSRIELILGDCVVQENWTSAGNIGY
                     SGKSYNIYNAALKRWEQYWVDYTGGNIFFYGGMKDGIMDYWTDELPQPDGKKLKRHLQ
                     FIKLVPDKVRQFSQGSNDGGKTWFVEYDFTYNRKK"
     gene            9369..9656
                     /locus_tag="VGR03_03380"
     CDS             9369..9656
                     /locus_tag="VGR03_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993352.1"
                     /translation="MKRRIFFTSYDWKALIWRFRRTLPQKGTPLAGLNSANSYNSLLN
                     LDDFLLYYPDFQIPSYLPATVRAGLNGFMKLIDEIARMNRKHSAGFYVYTP"
     gene            9690..10313
                     /locus_tag="VGR03_03385"
     CDS             9690..10313
                     /locus_tag="VGR03_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEV1993353.1"
                     /translation="MREPDEPTTSERILLSSGRYVNFGPFWIDQQRQEVTCNGSRLKL
                     QGKVYQILLTLLEKPGEVVTREELRIRLWPADTHVNYDANVNTTVNKLRQALGDSSDK
                     PLYIETIPRKGYCLLVQPEVSDKSPHPHFAQSEPFADSAQESSRNSDGLAFSKSDVWV
                     VLGVIGLILAGMLLGAGITRLWISHAAPVAPYPLAMIFSRASTTAFF"
     gene            complement(10322..11830)
                     /locus_tag="VGR03_03390"
     CDS             complement(10322..11830)
                     /locus_tag="VGR03_03390"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEV1993354.1"
                     /translation="MGADSMRRAGAILLVILGGAALWWAGNGAPASASANDAMVATIA
                     AARERAAANDPRLKGASRFEEGGWIYVHIEGDPGAMGYQHGYLLAPEIEDGFAAVSAG
                     MMHSTERDWEFFRKAAYEMLWPKIDAEYQQELQGIVEGLRARTGSKLDVYDLVAFNAF
                     EELPDYYVPWLNKQQKAANAPNLKSPGNCSAFVAAGSWTKDGQIVMAHNNWTNYRNGE
                     RWRIVFDIVPKNGYRMLMDGFPGVIASDDDFGINSDGLMVTETTITQFEGWDPNGKAE
                     FVRARKALQYAGSIDEYTKIMLDGNNGGYANDWLLGDRKTGEIAQLELGLKAHKLWRT
                     KDGVLAGSNWARDPKVLKEDAPQFDPNNKESSPNARRARWEELLKENKGKIDVALAEK
                     MLGDHSDTAQKKEEANERTLCGHVDGSPRGVKEWDWQPYYPGGAVQGKVTDATMAKEM
                     RLVARMGHPCGGDFLAKPFLETHPEQAWQEPYLRDMKAGPWTEFRSGQVPKE"
     gene            complement(11839..13317)
                     /locus_tag="VGR03_03395"
     CDS             complement(11839..13317)
                     /locus_tag="VGR03_03395"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV1993355.1"
                     /translation="MAIGRRIGGGFLASVLVFSGSNAAAARTAEDKKAVAVDEIFGDL
                     TKAGSPGCALGVYRDGKMVYSKGYGLANLEENVPITPQSVFDIGSTSKQFTAASVLLL
                     EKQGKLSINDDVRKYIPELPDYGQKITVLHLLNHTSGLRDYLTLMDLAGINTDSVTTD
                     EDALQMIFHQKALNFAPGSDWLYSNTGFFLLSVIVKRVSGKTLREFAAENIFVPLEMT
                     HTQYRDDHTSLIANRALAYDVRERGVGYSLDVSYFEQTGDGAVHTSVEDLLKWDENFY
                     SEQIGGKDFLSEIQERGKLNSGKVLDYSKGLFIEDYRGLHTVSHGGAWGGYRAELLRF
                     PERHFSVACLCNLGSAKPSNRAHRVADVYLASLMRPREVSKEMEEKPEKEKKLISQTA
                     EQLRSYPGDYWSDELGVAYRLGIVDGKLKVIALLDATGSPHTGNLPPHALEATAADNF
                     EMNEAQITIHFARDQHQGVKGFTLDAGRTRGMIFTRRDGVGK"
     gene            13408..14190
                     /locus_tag="VGR03_03400"
     CDS             13408..14190
                     /locus_tag="VGR03_03400"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEV1993356.1"
                     /translation="MLTAITRAVSPAIVNCELSFIDRQPIDLARAQEQHHAYEKLLEK
                     LGARIISLPAEPDLSDSMFVEDPAIVLDELAVILPLGTETRRREAPSLAQALAKFRKL
                     ETISLPGTLEGGDVLRIGRKLFVGLTKRSNAEGIRQLAAILAPHNYEVIAVPVTGCLH
                     LKSAVTHLGRNVLLANRQWFDITPLAGYEWIDVDPAEPHAANALALAGSVIFPASFPR
                     TRARIEARGFHVSPLDISELQKAESGLTCSSLLFEHTNSDGS"
     gene            complement(14303..14635)
                     /locus_tag="VGR03_03405"
     CDS             complement(14303..14635)
                     /locus_tag="VGR03_03405"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEV1993357.1"
                     /translation="MKTEHKVGRILGGTLLTMALSLGVANLQAQESRKVVSNPVPVYP
                     ETAKRMRLTGVVKVQVVIAPDGHIKETNVIGGHPLLVNAVQETLKNWKYGPASGETTT
                     QLEFNFHP"
     gene            15130..16218
                     /locus_tag="VGR03_03410"
     CDS             15130..16218
                     /locus_tag="VGR03_03410"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV1993358.1"
                     /translation="MSLENVQEESAAEPSPAALHDRVHCAGGVTKQPRNVVAFKFGGS
                     SLLGADRMLHAAGLVRAATRTSSVTVVVSAMKGVTDHLLSVARALADGKLAHARREAE
                     LVLHLHLDVLHDLQLTEGDDLRVRRELQLLGRDLLHEVPVHGSVAAGADLFDRLASFG
                     ERFSARLFASALEKSDVPAVPVTSSDFVLTCDTFRDAQPHLEQIKQRGRDVLLPLLEA
                     GVVAVVTGFIGATPDGRITTLGRNSSDFSGAIIAHVVDADELVIWTDVDGIYTANPNE
                     SAEARLLHELSYDEARALAASGAKVLHAKVLPLAAETELVVWVRNTFNPQARGTRIGS
                     PGSHRDPQIAPQDSVSILSISSQQGGAA"
     gene            16251..17369
                     /gene="asd"
                     /locus_tag="VGR03_03415"
     CDS             16251..17369
                     /gene="asd"
                     /locus_tag="VGR03_03415"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV1993359.1"
                     /translation="MQAKRPEKKWKAGVLGATGVVGQRLVQMLGNHPWFDLTEVAASE
                     RSSGKTYAEAVRWHLDAPIPEVARNLVVKGLDPSLDCDFVFSALDSSVAGPAEEVFAR
                     AGYPVVSNSRNHRTDPDVPLLIPEVNAAHLDAIPVQQKNRGYDTGFIVTNPNCSTAGL
                     VLVLKPLADAFGLEKVFVVTLQAISGAGYPGVPSMDIQGNVVPFISGEEEKMELEPQK
                     LLGKWDGSRFVDAGIGLSAHCNRVPVIDGHLECVSVGLKKIASLDEVREALRNFEVDA
                     ELASLPTALKNPVVVLDEENRPQPRRDVNAGNGMAAVVGRVRECPLLDVKLTLLSHNL
                     VRGAAGAALLNAELLAARGFLKHRVAPSESHLAEPVSR"
     gene            17501..20152
                     /locus_tag="VGR03_03420"
     CDS             17501..20152
                     /locus_tag="VGR03_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV1993360.1"
                     /translation="MALATGTKLGPYEIQSPLGAGGMGEVYRARDTRLERDVAVKVLS
                     AKLSSDPASRQRLDREAKAVSKLSHPHICTLHDIGHQDGVDFLVMELVEGETLDQRLT
                     KGFLPPEQTIRIAAQIADALAKAHKLGIVHRDLKPSNVMLTKTGAKLMDFGLAKEFGP
                     APLPDALTAMTAEQSKLTAEGMIVGTFQYMAPEQLEGKDSDVRTDIFALGALIYEMAT
                     GQPAFTGKSRASLIAAILSSEPQPMKALQSMTPPALERVVKKCLVKDPDERWQSASDL
                     ATELNWIAEGRSQAGEVGPLPAGRGKWERTGWLLAIAFFLLMIAGGVAWWQAGNRRPT
                     PMYFHASVPFSANDVALSPDGRTLAMVAYSTQANNYVLWTNEVGGRQTNSLVGTQDAS
                     YPFWSPDGRSIGFFADGKLKKVDVSGGQPQVLCDAPNGRGGTWNRDGMIVFAPDASGG
                     LFRLSSWGGSPVEMTKPDTSRFEESHRWPVFLPDGKHFLYLAANFSQELENDAIFLGS
                     LDSQERRWLVSTSANAAYAEPGYLLYLREKTLVAQPFDRRRYVLSGEPHPLSGEVRYF
                     PEVDRAVFSVSSGAVLVTQTGKGASLSQLTWFDRSGKSAGTVGIPGIYNNVRLSPDGR
                     RVAADQTDLDGRKIDIWIHGPARDAARRLSFDPGLDQTPIWSPDGRQILFNSNRKLSF
                     QLVLKNADGSGSEEEVTGASSNVVANVWDWSRDGKYILFGKGNELWSVSWPELVAKPL
                     LQAKWTVWNAQFSPDGRWIAYASNETGSMEIYVSPFSSANSKWQVSSAGAQEPRWRQD
                     GKELFYLSSERKMMAVAVKTTGASFEAGSPVALFQTYRRQPISAQDVFSYDVSGNGQR
                     FLIITKVDEANAVPPSVLLNWASAMEK"
BASE COUNT         3978 a         6384 c         5573 g         4400 t
ORIGIN      
        1 ttaagagcag gacttccagg gccgcgccga tcagaaccgc ggcgtgcagc gcgaccatcc
       61 aacggaaccg gggctcgtcg actttcgcgg cgccgcggga caccagtcgc tcttgatgcc
      121 gccgcgaaat gcgcagctcc ccaaggcgaa gcgccgccac ggcgaaaagc aagagcagaa
      181 aagcgatgac gcttatgtcc attgcaataa caaaagctcc atgctgaagc ccggcccgaa
      241 tccggccatt agaccgtaat ccccgggttt catcttcttc ttcgtcagcc actcctggag
      301 gataaacaga atcgtggcgc tggaaatgtt gccgtagtcg cgaagcacgt cccaggaaaa
      361 ctccgtgtgg caccggcgca ggcccagctc ttcctcgatg cagattagca gcttctggcc
      421 gccgggatgg agaaggtatg cggtcatctc ctggggcgtc aagttgtgcc gtccgaggaa
      481 attctccacc agccctttaa ttttgtcgcg caacaactgg ggaacatctt tcgagagcac
      541 gatgtggaat cccgtgtcgc ggagatcgaa gcccagagca tccatcgatc cggggagggt
      601 aaaggactcg gaatccagaa tcctcggtcc agaggaaggc cggttcgtga taaccgctgc
      661 cgcggcgcca tcgccaaaca gaaccgcgga aatcaaattg gcctgcgaga gatccttgcg
      721 ctgaaacgta agcgtgggca gttcgacggc gatgatcagc accgttttgc ccggagctcc
      781 gcgcaggaaa tccgccgccc gcgaaagcgc tatcgcgcca gcgatacagc ctaattcggt
      841 gatcggcagc cgccggatgt tgttgcgaaa gcccatcagg ttcaacagat gcacgtcgag
      901 cgagggaacc atatagccgg tgcacgagac ggtgatgatg aggtccacgt cggccggagc
      961 gacgccggcc ttcgccaggc actcctccgc agctcggcgt ccgagggcta tggcgtgttc
     1021 ctggtactcc tcattcgtct tggaaagcgg ccgcggctcg atcgtgtatt caatggggaa
     1081 gatgctgtgg cgcttgtgga cctgtgcgtt gtccacgacg gtcatcatgg cctcgagctt
     1141 gcggtcggga atgtcgaaca cgcgccccat gtaatacttc acgtcgtcgc gagtaatcgt
     1201 gtgcggcggc accgcggtgg ctgtcgcggc gatggtggga tacgaggctg cggcagtttc
     1261 taggggtcga ttgctcgcgg attccatcag tctccttggg cacaaaacgg gcgccggcgg
     1321 tttcccccgt cgcgtaagcc ttttgaatgc agcgctttct gggtagtctg cattcgagca
     1381 aacgacttta catcaattca ttgccgggag cgaactagtt tgacggggga gtcggtccca
     1441 ggcaagagaa aaccgtgatc ggaaacttgc gcgaagtcgc tgagcggctc gcacccggtc
     1501 gcgcctgcat atcctttgtt tccgaccgcg gctttgcaaa gaccgggacg ccattgaatt
     1561 tactcccgag gcgcggcggt gcagaggtca ttggctgagc atgatagatt ctctgactgg
     1621 tgaggtgctt catgcgggga actgcatttc gtcacactgt gttaggcgca gtgggcctct
     1681 tgctagtgag cttgccggtg cgcggtcagg tgtcccagtg gcgtggggca accgaaggcg
     1741 atttcgtcgc caagaaattc aaattccggt cgggcgagat gctgccggaa ctgcgcttgc
     1801 actacacgac gctgggcaaa cccgctcgca acgcggaggg ccgcgtcacg aacgcagtgc
     1861 tgattcttca cggcaccggt ggcacgggcc agcaatttct ccagccgcaa tttgccggag
     1921 agctctttgg gccgggtcag ttactcgatg tcacccgcta cttcatcatc tttcccgacg
     1981 gcgttggcca cgggaagtcc tcgaagccca gcgacgggct gcatgctcat ttcccgcaat
     2041 acgactatga cgacatggtc gccgcgcacc accagcttct cagcgagggc ctcggcgtaa
     2101 accatctgcg cctcatcctt ggcacctcca tgggctgcat gcattccttt gtgtggggag
     2161 aaacctatcc tgatttcatg gacgcgctga tgccgctggc ttgccagccg gtgcagatcg
     2221 cgggacgcaa ccgcgtctgg cgcaaaatgg taatggacgc gattcgcaac gatccggaat
     2281 ggaagggagg agagtactcg gcggagcccc agcaaggatt acgcactgcg ctcgaccttc
     2341 tgctcattgc cggcagcgct ccgctctaca tgcagaaaaa tctgccgacg cgagacaccg
     2401 cggacaaata cctggatgac tatttcaaga cgcgttttgc cgggctggac gccaacgatc
     2461 tcctgtatca ggtgaatgct tcgcgcaatt acgatccttc gccgcagctt gagaaaatca
     2521 ccgcgcatgt catgtacatc aattccgtgg acgacttcat caacccgccc gagctgggca
     2581 tggccgaacg cgaaatcaag agggtaaaaa atgcgcgctt cattctgatc cccatcagcg
     2641 acgaaacgcg cggccacggc acgcatacgc gggctgtggt gtggaagcag tacctggtgg
     2701 aactgctcga aaaatcagcg cactaaaaag ctcggagcgg tgctggatct aggtggtagc
     2761 ttggctggcg cggatttcct gccagcggcg gggccgcttg ttcgcttgga gaactttctt
     2821 gcgcaggcgg atggatttcg gcgtgatctc cacgtactcg tcgtctgcga tgaactcgat
     2881 ggcctgctcg agactcaact ggcgatgggg aatcaagcgg atcgcttcgt cggcgctgga
     2941 cgagcgcatg ttggtctgct tcttttcctt tgttacgttg acgtccatgt cctgttcgcg
     3001 tgaattttcg cccacgatca tgccttcgta gacatcgatg gccggcccga cgaacaactc
     3061 gccgcgctcc tggatgttcc agagcgcaaa cgcggtagat ggcccgccgc ggtccgccac
     3121 caacgcgccc gttgggcgca gcgccatctc tccgccgtat tccatccagc cttcgaaaat
     3181 cgaatgaatc agcgccgtgc cgcgcgtgtc tgtgagcaac tggctgcgga gcccgatcaa
     3241 tccgcgtgac ggaatcttga agtccatgcg cacgcggccc gagccgtgat tgctcatctt
     3301 gatcatctcg ccgcgccggc taccgatcgt ttccatgatg attccgatga aattctcggg
     3361 tacgtcgatc accagccgct ccagcggctc gagtttcttg ccgttttccg tgcggacgac
     3421 aatctcgggc ttgcccacca tcagttcaaa gccttcgcgc cgcatcgttt cgatcaaaat
     3481 cgcaagctgc aattcgccgc gccccagcac gcggaaggat tccggcgtgt ctgtatcttc
     3541 cacgcggatg gacacatttg tcagaagctc tttttgcagg cgatcgcgga ggtcgcgcga
     3601 agtgagaaac gttccgtctt tgccggaaag cgggccgctg ttgacggtaa aaatcatcgc
     3661 cagcgtgggc tcgtcgatca gcagcggttc cagcggcatc ggattcgcca aatccgtaat
     3721 gctttccccg atgttgatgc cctcaacgcc ggcaattgcc accagatcgc ccgccgggac
     3781 ggcttgcgct tcatcccgct ccaggccgcg aaaggtatac agcttggtga tgcgcgtcgg
     3841 cgtcagccgg ccgtccagct tgacgatgcc cacgtccgcg ccgcgcctga gcgtgccgtt
     3901 gaatacgcgc ccgatcgcga tgcgtcccag aaattcgctg taatccaggt tcatcacctg
     3961 aatctgaaga actccttccg ggtctcccgg aggagcagga atgtgtttta cgatggtctc
     4021 gaaaagcggc agcagatctt tcgagtcgtc acccggcgtc atgtgcgcaa cgccgtcgcg
     4081 cgcatttgtg tacaccaccg gaaagtcgag ctggtcttcc gttgcgtcca ggtcgataaa
     4141 gagatcgtag atttcgtcca ggacttcctt ggcgcgcgca tcggtgcggt cgatcttgtt
     4201 cagcacgatc accggaggca gcttcgccgc cagcgccttt tgcaggacgt agcgcgtctg
     4261 cggcaacggc ccttcgctgg cgtcgacgag caccacaacg ccgtccacca tgcgcaacgc
     4321 gcgctccact tcgccgccaa agtcactgtg ccccggcgtg tccacgatat tgatcttcgt
     4381 gtcgtggaag aaaagcgcgg tgttcttcgc gagaatggtg atgccgcgct cgcgttccag
     4441 gtcgttcgag tccatcacgc gctcaatgag ggcctcgtta gcgcggaaaa tcccgctttg
     4501 cctcagcatg gcgtccacca gcgtggtctt gccgtggtcc acgtgcgcga tgatggcgat
     4561 atttcgaata ttttgacgca tagtttctgc ttcttctgac cccgaccggg tcgggttcaa
     4621 gtcctctaaa gacaaaaata gctgccgaaa caaccagcag ccttgacgaa tgtacctttt
     4681 ttgtaggttg accgcctggc ggtcacacat ccttaggtgc ggtgcagcaa gctatagttt
     4741 agcagactcg ctgcttgacg gccagcagtg gaggcgagaa gaggttccgt attcgcacgg
     4801 cacttaagtc cttgttcggt gtctaattgt aaggtgagat ctccactcct aatcggtctt
     4861 ttctgtggac ttatggtctc tgccgctgcg ctagggcaga agaagcctga caccgccgtc
     4921 aggattattt gtccactcct acatccgaaa atcatcaaga tggttcgacc aatttatccc
     4981 gaattggcga gacaagctca cgtcgacggt actgtttcct tgaagtgcct cgtcggactt
     5041 gacggatcag tagagaatat caaagtcaag aagggcctcg cactactcat tcgcgcagcg
     5101 actgaggcag ttgcgcaatg gaaatacaag ccgctgcttc tcaacggaaa ggccgtcgag
     5161 acagatacgg tcgtgaacat tattttccaa ttgcccaagg aacagaagaa gacagatgct
     5221 ctcactcaca aattagtgcc ttcgctctat tccacgcgga gggcgatgat ggggtccacg
     5281 cgagccgcac ggcgcgcagg aacgtagcag gccacgagcg cggccaggcc gagcactagc
     5341 gcaactccga cataggtcag cggatcggtt ggcgctactt caaagagcaa ttgctccaag
     5401 aggcgcgtga gccacagagc accgaacagg ccgagcgcga cgccaaccag agccagcgtc
     5461 atgccctgcc ccagaatcat gcgcaggatt tctcctggac gcgcgccgat ggccatgcgc
     5521 actccgatct catgccggcg ctggccgacc gccaaggcca tcacgccacc gatacccgtg
     5581 gccgcaatcg ccagcgccag caaagcgaac aatcccagca aactggtcgt caacctgggt
     5641 gccgcgacgg attccgcgcg cacctgttcg agcgaccgca cccgcgcggc cggctgattc
     5701 ggatcaacgg catagagcaa ctcgatgacg ctgcgcgcga tgctcatcgg ctccgccgtc
     5761 gtcttcacga ccaggacggc gtccattagc gggttctgcg ccatcggcac gtagatttcg
     5821 tccacgggag ctttgtccag tccgtactgc ttcacgtcac ccacgactcc ggcgatctgc
     5881 acccaagtct ttccgccatc cgccgaaaac cgttttccga cgggatcctg cgtaccccag
     5941 aagtgccgcg ccgcggcgcg attgacgatg gctacatcca gcgcgcctgg acgatccgcg
     6001 cgcatgaacg cccgacccgc aagcaccggg atgtgcagcg tctcgaaata gcgctcgctg
     6061 accacgcgga aatccgaaac gggaagcgct tgcccgggca cgggtgtttg cccttcgatc
     6121 tggaaatcac cggtcatgcg catgttgccg ttcaatggaa tcatcatcga ggccgccgca
     6181 gatttcactc cactttgcgc ctcgatttta tcgaggaacg tttcgaagaa ggctcgctgc
     6241 ttgtcgttcg tgccgtactt ggtgaagtcc agaccgattt gcatcgtcaa cacgttttcc
     6301 ggctggaagc ccgggtccac gtgctgcagc ttcgcgaagc tgcgcagcat caaacccgcg
     6361 ccaatcagaa ggaggaacga cactgccact tgcgccacga tgagcaagct cctcatttgg
     6421 tgcctgccgc gcccgatggt cgattgcgcg ccaccttctt tcaggctgcc cacaaccgtc
     6481 tcgcgcgcgg ccagcgctgg agccgtcccg gaaagcaagc tcgtcagcac ggccacggca
     6541 agcgtgaaca gcagtaccgt ggcgtccaga tgaatctctc tcgcccgcgg agtgaagcgc
     6601 gccgcaaaac tcaccagcag gttcagcgct ccccacgaga ataaaagccc caaaccgccg
     6661 ccgagcagcg cgagcagtga actttccgtc agcaattggc ggaacatccg cgcgcgtccc
     6721 gcacccagcg ccgcgcggac ggccagctct cgttcccggc gcaccattcg cgagaggttc
     6781 aggttcgcaa cgttggcgca cgcgatcagc agcacgaacc ccgccgcacc gaggagcact
     6841 agcatcgtcg ggcgcgcgtt gtgcgtgagc tcttccgcga gcgaggacgt ccccagcgag
     6901 taatcgatag atggcggata atcgttcgga tacgccttct gcaaattcgc ggcgacacca
     6961 ttcaagtcgg cctgcgcctg gctcagcctg attcccggct tcatgcggcc gaacgcttgc
     7021 atcatgcggg attgccggtt ggcgatgaat gcgggacgcg agcggaaggg gcacgccgtc
     7081 gtcggcatgt acacatcgtt ctcgtccgga tactgcggta caggcggcag tatgcccacg
     7141 acggtgtgca gccggtcgtt catggtgaac gatttgttta caaccgtcgg atcgccgccg
     7201 aagctcctaa tccagtattc gtaactgagc atcagaaccg ccggtgcacc gggctgttcg
     7261 tcttccggac gaaaggcgcg gccgaagagc ggcttcacgc cgaacacgtc aaagtagttc
     7321 caggaaacga ccccggtttc cacgcgttcc ggctcgctgc gtcccagcag aatgaaattc
     7381 atgttgtggt attcgaccag accgtccagg gaatggctct gcgtccggta gtcgttgatc
     7441 tccgccaccg agaagggttg attcagcatt cccgcgcgcg gcgcctgctg gtgcagcgtc
     7501 accaggcggt cgccgtgttc gtacggcagc ggcttcagca gcaccgcatt cacgacactg
     7561 aagatggccg aattggcgcc gactcccagt cccaaggtga ggatcgccgc aagcgtgaac
     7621 cccgggctct tgcgcatcat ccgcaacgcg aatcccgcat cctgacggaa catcgtcagg
     7681 tgctcgcccg aagccgttcg gaaaattccc acaattgttt cccaccacag acgcagaaca
     7741 cccgtggctc cgttgcggcg ccccgcttcc ttgtgttgtt ccttaaaaac cgtttccatt
     7801 tcgggaccga agtcgctccg aaaatcgaag ggaagaagcc gcagaagtgc tcgataaatt
     7861 cgtgtcgctc tggaatcagc cgctgccatg atgtcctcct gcggcgccac cacagcaccg
     7921 attttcgggc cactcgcttt tcgcggagcc atctcgtcag gctccggcaa tcttgttacg
     7981 ccggccgcgt tttcttcagc aggtttctcg accgcgcgat ggacaggatt tcgtccatcc
     8041 tctccgcttc cgcggttgcc acctgccggc cttggggcgt caatcggtaa tagcgtcgcc
     8101 gctcatcgtc ttctgcctcc gcgggccgcg cacgcagttc aacgatcaag ccatcgttca
     8161 gcaagcgctt gatgattcca tagagagttc ccgtgctcag ccggaccttg ccgccggtgc
     8221 gcagctccac ttctttgagg atggcatagc cgtggagatc gtcgccggcg agcgccagca
     8281 gcacatggaa catggaaggc gtcagtggcc cgaacggttc ggcgtggctt tgagcagcag
     8341 tcaagggtta gctccacaga aataatgttc tgtcgtcata cgctatatcg tcacacgcca
     8401 tagtgtcaag cggaaaatgc cctgggcatc ctgcaacccg cgaatcctat tttttccggt
     8461 tgtaggtgaa gtcgtactcc acgaaccagg tcttgccgcc atcattcgaa ccctggctga
     8521 attgccggac cttgtcaggt acaagcttga tgaattgcag atgccgtttt agtttcttgc
     8581 cgtctggttg cgggagttca tcggtccagt aatccatgat gccgtccttc attccgccat
     8641 aaaagaaaat gttgccgccg gtgtagtcca cccaatattg ttcccaccgc ttgagcgccg
     8701 cgttgtaaat gttgtagctc ttcccggagt atccgatgtt ccccgcactc gtccagtttt
     8761 cctgtaccac gcaatccccg agtatcaatt cgattcggct gtctcccgcc agcgtctctc
     8821 cctggctcgt gaccacgctc cattcgccca gccagaaatc aaattgacga ttttccgccg
     8881 cataagcgca cggtttttgg ttgcgcgtca caagttcgag gatttttgca aagcgcggat
     8941 cggacttcag ttctgccaga tcaggatcgg ctgacaattg gtcaggttgt cccaagccat
     9001 tttgagccgc cttttccagg tgttcaaacg ccttatcctt gtctttttta gatacgaaga
     9061 cggtggcgat tccatagtca gcgaattgcg gcggcagtcc caatgctctg cctttttcga
     9121 aggcctcgat ggccttctcc ttttccccgg tcttatttaa ggacacacct aaccggtacc
     9181 acgcgcgtgg attcggcgcc gtttgcacga tctggccgta tagcttggcg gactcactcc
     9241 agttctttgc gtcatatgct gacgccgctt cggtggccac ttgagttatc gaagacgcct
     9301 gcggcggacg ccccagcgcc atcgctgcca cggacaggag cagcaaagca tttgctgcat
     9361 agagcgtcat gaaacgccgc attttcttca cctcatatga ctggaaagct ctgatttggc
     9421 gttttagacg cacattgcca caaaagggca ctccgctggc gggattgaat tccgctaatt
     9481 catataattc acttctaaat ttagacgatt tccttctgta ctatcccgat tttcagattc
     9541 cttcctatct ccctgcaacg gtgcgggccg gactcaacgg tttcatgaaa ctaattgatg
     9601 aaattgctcg tatgaataga aaacactcgg ctggtttcta cgtctataca ccataagggt
     9661 ggacgtctat gtcaccggaa gtgatctcga tgcgagaacc ggatgaaccg acgacttcgg
     9721 agcggatact cctgtcatcc ggccgctatg tcaattttgg gccgttctgg attgaccagc
     9781 agcggcagga agtgacgtgc aacgggtccc ggctcaagct ccagggcaag gtttatcaga
     9841 ttcttctgac actcctcgaa aaaccgggtg aagtcgtcac ccgcgaagag ctgcgcatac
     9901 gcctctggcc cgccgatacg cacgtgaact acgacgccaa cgtaaatacc accgtcaaca
     9961 aactccgcca ggcgcttggc gattcctccg ataagccgct ctacattgag accattcctc
    10021 ggaaaggcta ctgcctcctg gtgcagccgg aagtctccga caagtcccca catcctcact
    10081 tcgcccagag cgaacccttt gctgattccg ctcaagaatc ttctcgaaac tcggacggcc
    10141 tggcgttttc aaaatcggat gtttgggttg tgctcggcgt gatcggactg attcttgccg
    10201 ggatgctcct cggcgcggga attacccggc tctggatttc ccacgcggcg cctgtggccc
    10261 cttacccctt ggcgatgatt ttttcgcgcg catcaacgac tgcgtttttt tgagacttgc
    10321 tttactcttt cgggacttgg cctgaccgaa attccgtcca cgggcccgcc ttcatatcgc
    10381 gcaaataagg ctcctgccag gcctgctcgg gatgagtctc aagaaacggc ttggccagaa
    10441 aatccccgcc gcagggatga cccatgcgag ccacgagtcg catttccttc gccatcgttg
    10501 catcagttac ttttccttga accgctccgc cgggatagta gggttgccaa tcccactctt
    10561 tcacgccgcg cggcgatcca tccacgtgcc cgcacagcgt gcgctcgttg gcttcttcct
    10621 tcttctgagc cgtgtccgag tggtcgccca gcatcttttc cgctagcgcg acgtcgatct
    10681 ttcctttgtt ttccttcagc aattcctccc accgcgcccg ccgcgcgttc ggcgaacttt
    10741 ctttattatt tggatcaaac tgtggcgcat cctccttgag caccttcgga tcgcgcgccc
    10801 agttcgatcc cgcgagtacg ccatccttcg tcctccacag tttgtgcgct tttagcccca
    10861 gctcgagttg cgcgatctct cctgtcttcc ggtcgcccag cagccagtcg tttgcatagc
    10921 ccccgttgtt tccgtccagc atgatcttcg tgtattcgtc gatcgagccc gcatactgca
    10981 gcgctttccg cgctcgcaca aattccgcct tgccattcgg gtcccagcct tcaaactgcg
    11041 tgatcgtcgt ttccgtcacc atcagcccgt cgctgttgat cccgaaatcg tcgtcgctcg
    11101 cgatcacgcc cgggaaacca tccatcagca ttctgtaacc gttcttcggc acgatgtcga
    11161 acacgattcg ccaccgttcg ccgttccggt agttcgtcca gttgttgtgc gccatcacga
    11221 tctgcccgtc tttcgtccag cttcccgccg ccacgaaagc gctgcaattt ccggggcttt
    11281 tcagattcgg cgcattcgct gccttttgct gcttgttcag ccagggcacg taatagtccg
    11341 gcagctcctc gaaggcattg aacgccacga ggtcgtatac atccagcttc gacccggtgc
    11401 gtgcgcgcag tccctcgacg atgccctgca gctcctgctg atactccgca tcgatcttcg
    11461 gccacagcat ttcgtacgcg gctttccgga aaaattccca gtcgcgttcg gtggaatgca
    11521 tcatccctgc gctaactgcc gcgaacccat cttcgatctc cggcgcgagg aggtacccgt
    11581 gctgatatcc catcgcgccc ggatcgccct cgatatgcac gtagatccag ccgccctctt
    11641 cgaagcgcga cgctcctttc aaacgtggat cgttcgccgc cgcacgctct cgcgccgccg
    11701 cgatcgtcgc caccatggcg tcattcgccg aagcggaagc cggcgctccg ttccccgccc
    11761 accacagcgc cgcccctccc aggatcacca gcagaatcgc tcccgcccgc ctcatcgaat
    11821 ccgcacccat cttccctcct actttcccac gccgtctctg cgagtgaaaa tcataccgcg
    11881 cgtccgcccc gcatccaggg tgaatccttt cacgccttga tgttgatccc ttgcgaagtg
    11941 aatcgtaatt tgcgcctcgt tcatctcaaa attatctgcc gccgtggcct cgagagcgtg
    12001 cggaggaaga ttgccggtgt gcggagagcc tgttgcatcc aacagagcga taaccttcag
    12061 cttgccatcc acaattccca gccggtatgc gacacccaat tcgtcactcc agtagtcgcc
    12121 cgggtagctt ctgagctgtt ctgccgtctg cgaaatcagc ttcttctctt tctcgggctt
    12181 ttcttccatt tccttgctta cttctcttgg cctcatcagg ctggccagat agacatctgc
    12241 aacccgatgt gcgcgattcg atggcttcgc gcttcccaga ttacacaggc acgccaccga
    12301 aaagtgccgc tctggaaaac gcagcaattc cgcgcggtat ccgccccacg ctccaccgtg
    12361 gctcaccgtg tgaagtccgc ggtagtcttc gatgaataat ccctttgagt aatccagcac
    12421 cttgccgctg ttcagtttgc ctcgttcctg aatctccgag aggaaatctt tcccgccgat
    12481 ctgctcgctg tagaaatttt catcccactt cagcaggtcc tccactgaag tgtgaaccgc
    12541 gccatcaccg gtttgctcga agtacgaaac atccaggctg taacctacgc ctctttctct
    12601 cacatcgtag gccagcgcgc ggttcgcaat caacgacgta tggtcgtcgc gatactgcgt
    12661 atgggtcatc tccagcggca cgaagatgtt ctccgccgcg aattcccgca gcgtctttcc
    12721 gctcacgcgt ttcacgatta ccgacaatag aaaaaatccc gtgttgctgt aaagccagtc
    12781 gctccccggc gcaaaattca gcgccttctg gtggaaaatc atctgcaacg cgtcttcatc
    12841 cgtcgttacg ctgtccgtgt tgatgcccgc cagatccatc agcgtaagat aatcgcgcag
    12901 accgctggtg tggttcagca gatgtagtac cgtgattttt tgtccgtaat ccgggagttc
    12961 aggaatgtat ttccggacat cgtcgttgat ggagagtttt ccctgctttt ccagaagcag
    13021 aacgctcgcc gccgtgaact gtttggatgt ggaaccgatg tcgaacacgc tctgcggagt
    13081 tatcggcaca ttctcttcaa gattcgcaag gccatagcct ttggaataga ccatctttcc
    13141 gtcgcggtag acgcctagcg cgcatcccgg tgagcctgct ttcgtcagat ccccgaaaat
    13201 ttcatctact gccactgcct ttttgtcttc ggcggttctc gcagccgccg cattcgagcc
    13261 gctgaaaaca agcacgctcg ccagaaatcc tccgccgatc ctgcgcccaa tcgccattcg
    13321 atttgctcct cgatcaagtt cggtggcgag tctatacact cctccgctgt ctctcaagag
    13381 aatctcgtgt aaactccctt gcttgtcatg ctcaccgcca tcacacgtgc cgtcagccca
    13441 gccatcgtca attgcgagct ttccttcatc gatcgccagc ccatcgatct agccagggcc
    13501 caggagcagc accacgccta cgaaaagctt ctggaaaaac tcggcgcacg aattatttcg
    13561 ctccctgccg agcccgatct ttccgactcc atgttcgtcg aagatcccgc gatcgttctc
    13621 gatgaattgg cggtgatcct gccgctcgga accgagaccc gccgccgcga ggcgccgtcg
    13681 ctcgctcagg cgctcgccaa atttcgaaag ctcgaaacca tttctcttcc cggcacactc
    13741 gaaggtggcg acgtccttcg catcggccga aagctcttcg tcggcctcac caagcgcagc
    13801 aacgccgaag gcatccgcca gctcgccgcc attcttgctc cacacaacta cgaagtcatc
    13861 gccgttcccg ttaccggctg ccttcatctg aaatccgccg tcacgcatct cggccgcaac
    13921 gtgctcctcg caaaccgtca gtggttcgac atcacgccac tagctggtta cgaatggatc
    13981 gacgtcgatc ccgccgagcc tcatgcagcc aatgccctgg ccctcgcggg ctcggttatt
    14041 ttccccgctt ccttcccacg cacacgtgca cgcatcgaag cgcgcggctt ccatgtttcg
    14101 cctctcgaca tttccgaact ccaaaaagcc gaatccggcc tcacctgctc cagcctattg
    14161 ttcgaacaca cgaattcgga tggctcatga tgcggcagat gcaaggacgg gggttgcagg
    14221 gaatcgtcag cctccattga caaaaacggc cgcgcggaag tcatccgcat ggccgttttc
    14281 ttactcgact cgttcccccg tactacggat ggaaattgaa ttccagctgc gtcgtcgttt
    14341 ctccgctggc gggcccatac ttccagttct ttagcgtctc ctggaccgca ttgacaagca
    14401 acgggtggcc gccgataacg ttcgtttcct ttatatggcc gtccggggcg atcactactt
    14461 gcacctttac gacgcccgtc aggcgcattc tcttggccgt ttcgggatat acaggaacgg
    14521 gattagaaac caccttgcgg ctttcctgcg cctgcaaatt cgctacgccc aggctaagcg
    14581 ccatcgtcag caacgtgccg cccaggattc ttcctacctt atgctccgtt ttcattttca
    14641 actcttcctt ttgacctagc gtttagccat cgttcgaacg ctcttggatt tgccatgctc
    14701 ggggcttggt tccaaactac cgggaatccc ttgcttccgc attcttctcg cgggacaggt
    14761 gtttctcccg aaaaactgct cgctcggact gggggaaaac ctgtagaagg gtcagctagc
    14821 ccgcttgccg cacctccgcc cccatttcca aagttttcct tctcgtccac cctagcctgt
    14881 gctattctct tcccgtgatc ttgtccatcg cctccaagcg aattacgtcc aagtcgtcca
    14941 tgtcgattac ctcgactacg actaagacga ctatttccgc ttcgggcgcg tggagaaaca
    15001 gctagcgaac tagcaaaaag tttccaaccc accgccagaa gcgaacctgg cggtgggttt
    15061 cttcttttaa accccctcca gagacgctcc agcggttgta atcgcaaagg aaaaccagga
    15121 gcgcaggtca tgagtctcga aaacgtacag gaagaatcag cagcggaacc aagccccgcc
    15181 gcgttgcatg atcgagtgca ctgcgccggc ggggtcacca agcaaccacg aaacgtcgtc
    15241 gccttcaagt tcggcggcag ttctcttctc ggcgcggacc gcatgctcca tgccgcgggc
    15301 ctcgttcgcg ccgccacgcg aacatccagc gtcaccgtgg tcgtctccgc catgaagggc
    15361 gtcaccgatc atcttctttc cgttgctcga gcgctcgccg atggcaaact cgcgcatgcc
    15421 cgccgcgaag ccgagctcgt tctgcatctt cacctcgatg tgcttcacga tctgcagctc
    15481 acggagggcg atgaccttcg cgtgcgccgc gagttgcaac tcctgggacg cgaccttctt
    15541 cacgaagttc cggttcacgg cagcgtggcc gccggcgcgg atctcttcga tcgcctcgct
    15601 tcctttggcg aacgcttcag cgccaggctc ttcgcctccg ctctcgaaaa gtcagatgtc
    15661 cctgcggtgc ccgtcacgtc ttcggatttc gtcctcacct gcgacacctt ccgtgacgcg
    15721 caaccgcatc tcgaacaaat caaacaacgc ggccgtgacg ttcttcttcc gctcctcgaa
    15781 gctggcgtcg tggccgtggt taccggcttc atcggcgcaa ctcccgatgg ccgcatcacc
    15841 acgcttggcc gcaacagctc ggatttttcc ggggccatca tcgcgcacgt cgtggacgcc
    15901 gacgaactcg tcatttggac cgacgtcgac ggcatttaca ccgcgaatcc gaacgaatcc
    15961 gccgaagcgc gcctgttgca tgagctcagc tacgatgagg cccgtgccct tgccgccagc
    16021 ggcgcgaaag ttctccacgc aaaagttctg cctctcgccg ccgaaaccga attggtcgtc
    16081 tgggttcgca atactttcaa tccgcaagcg cgcggcacgc gcatcggttc gccgggctcg
    16141 caccgggatc cgcaaattgc gccacaggat tccgtttcga ttctttcgat ttcatcccaa
    16201 cagggcggtg cggcatgaat tcaccatctc aaaacaacgg agatcaaaat atgcaggcga
    16261 aacggccgga gaaaaagtgg aaggcgggag ttctcggtgc tactggagtc gtcggccagc
    16321 gcctcgttca aatgctcggc aatcatccgt ggtttgacct cactgaagtt gccgcctctg
    16381 agcgttcttc cggcaagacc tacgccgaag ccgttcgctg gcatctcgac gcgcctatcc
    16441 cggaagtggc tcgcaatctc gtcgtcaaag gtctcgaccc ctctctcgat tgcgattttg
    16501 ttttctccgc gctcgattct tccgtcgctg gccctgccga agaagttttc gcccgcgccg
    16561 gatatcccgt cgtcagcaat tccaggaatc accgcacgga ccctgatgtt cccttgctca
    16621 ttcccgaagt gaacgccgcc catctcgacg ccattcccgt tcaacagaag aatcgcggct
    16681 acgacaccgg cttcatcgtc accaatccca attgctccac cgccggcctc gtccttgtat
    16741 tgaaacctct cgccgatgcc tttggcctcg agaaagtttt cgtcgtcact cttcaagcca
    16801 tctccggcgc cggctatccc ggtgtccctt cgatggatat ccagggcaat gtcgttccct
    16861 tcatcagcgg tgaagaagaa aaaatggagc tcgagcctca gaagctcctc ggcaaatggg
    16921 atggctcgcg gttcgttgat gccggcatcg gtctcagcgc ccattgcaat cgcgtgccgg
    16981 tgatcgacgg ccatctcgaa tgtgtctccg tcggcctgaa gaaaatcgct tccctcgacg
    17041 aagtccgcga agccctccgc aacttcgaag tcgacgccga gctggcctcg ctgcccaccg
    17101 cgctcaaaaa tcccgtcgta gtcctagacg aggaaaatcg cccccagccg cgtcgcgacg
    17161 tcaacgccgg caacggcatg gccgccgtcg tcggccgcgt ccgcgaatgc cccttgctcg
    17221 atgtaaaact cacactgctc tcgcacaacc tcgtccgcgg tgccgccggt gccgccttgc
    17281 tcaacgccga gctcctcgcc gcccgcggct tcctcaagca ccgcgtcgcc ccaagcgagt
    17341 cccacctcgc cgaacccgtc tcgcggtagc gtgcaccgct gcggtcttgt aggggcaaac
    17401 ttcagttcgc cctgttttcc cttgtagggg cgcagcacca ctgcgcccgc ttcggctgct
    17461 cgggtaatga ttcagtttga gggtataatg cgcgccaacg atggcgctcg cgactggcac
    17521 aaaacttggt ccctatgaga ttcaatctcc actcggcgcg ggtggcatgg gcgaagtgta
    17581 tcgcgcccgc gacacgcggc tcgagcggga tgtagccgta aaggttcttt ccgcgaagct
    17641 ctcctccgat ccggcttcac ggcaacgcct cgaccgcgaa gcgaaagctg tttcgaaact
    17701 ttctcatccg cacatctgca ctctccatga catcggccat caggatggcg tcgacttcct
    17761 cgtcatggaa ctggtcgagg gtgaaacgct ggaccagcgc ctcacgaagg gatttttgcc
    17821 gcccgagcag accatccgca ttgcggctca aattgccgat gcactcgcca aggcccacaa
    17881 attgggtatc gttcaccgcg atctcaaacc ctcgaatgtc atgctgacga aaaccggcgc
    17941 caagctgatg gattttggtt tggcgaaaga gttcggccct gctcccttgc cggatgcgct
    18001 gacagcgatg acggcggaac aatcgaaatt gaccgccgaa ggcatgatcg tcgggacgtt
    18061 tcagtacatg gcgcctgagc aactggaagg caaagactct gacgtgcgca cggatatctt
    18121 cgcgctaggc gcactgatct acgaaatggc caccggccag ccagcattta ccgggaagag
    18181 ccgcgccagc ttgatcgccg ccattctttc ttccgaacca cagcctatga aggctttgca
    18241 gtccatgacg ccgcccgcgc tggagcgcgt cgttaagaaa tgcttggtga aagatcccga
    18301 tgaacgttgg caaagtgcca gcgacttggc aaccgagctg aactggatcg ccgaaggacg
    18361 ctcgcaggct ggagaagtcg ggccacttcc ggcgggccgc gggaaatggg agcgtaccgg
    18421 ttggcttttg gccatcgcgt ttttcctgtt gatgatagcc ggtggtgtgg cgtggtggca
    18481 ggccggcaac cgtcgcccga cccctatgta tttccacgca tccgtgccct tctccgcgaa
    18541 cgacgtggct ttgtctccag acggtcggac gctggcaatg gtcgcctact ccacgcaggc
    18601 gaacaactac gtgctctgga cgaatgaagt cgggggtcgg cagaccaatt cgttggttgg
    18661 gacgcaggac gcgtcctacc cattttggtc accggacggc agatccatcg ggttcttcgc
    18721 tgacggcaaa ctcaagaaag tggatgtgtc aggaggacag ccccaggtgc tgtgcgatgc
    18781 gcccaacggg cgcggcggca cgtggaaccg ggacggcatg atcgtattcg cgcccgacgc
    18841 atccggaggg ttgttccgat tgtcttcctg gggagggtct ccggttgaaa tgaccaagcc
    18901 ggacacgtca cgctttgaag aaagccatcg ctggccggtg ttcttgccgg acgggaagca
    18961 ttttctttat ctggctgcga atttctccca ggagttagaa aatgacgcga tcttcctggg
    19021 ctcactggat tcgcaggaaa gacgctggct ggtgagcacg agcgcgaacg cggcctatgc
    19081 ggagccaggc tacctgctgt acctgcggga aaagacattg gtggcgcaac cgttcgatcg
    19141 gcggcgctat gttctgagcg gcgagccgca ccccttgagt ggcgaagtgc ggtacttccc
    19201 cgaggtggac agggctgttt tcagtgtctc cagtggggcg gttctggtca cgcaaacggg
    19261 aaagggcgcg tccctttcgc agttgacgtg gtttgaccga agcggaaagt cggcgggcac
    19321 ggtagggatt cctgggattt acaacaacgt acgtctgtcg ccagacgggc gccgggtcgc
    19381 cgcagaccaa accgacctgg acggacggaa gattgatatt tggatccatg ggccggcccg
    19441 ggatgcggcc aggcggctgt cgttcgatcc ggggttagac cagacgccta tctggagtcc
    19501 ggatggcagg cagatactgt tcaattcgaa ccgcaagctc agcttccagc ttgttctgaa
    19561 gaacgccgat ggctccggct cggaggaaga ggtgaccggt gccagctcca atgtagtggc
    19621 caatgtctgg gattggtcgc gcgacggcaa gtacatattg ttcgggaagg gaaacgagct
    19681 gtggtccgtc tcctggcccg agctcgtagc caaaccgctg ttacaggcaa agtggaccgt
    19741 gtggaacgca cagttttccc ccgatggccg atggatagca tacgcctcga acgagacggg
    19801 gagcatggag atctatgtgt cccccttttc gagtgccaac agcaagtggc aggtctcgag
    19861 cgcaggagca caggagccga ggtggcgcca ggatggcaag gagttgttct acctgtcgtc
    19921 ggaacgaaag atgatggcgg tggcggtgaa gacgacgggc gccagcttcg aagccggctc
    19981 cccggtcgcg ctgtttcaga cctaccggcg acagccgatt tccgcgcagg acgtcttctc
    20041 ctacgatgtg agtggcaacg ggcagagatt cctgatcatc acgaaagtag acgaagccaa
    20101 tgctgtgccg ccctccgtcc tcctaaactg ggcttccgcg atggagaaat agcaccgccc
    20161 gcgcgccagc gaccaccctc ccggcaagtg cattacttcc gagtagcgcg tgagaacgaa
    20221 gccgcccgcg attgcggcgg acgcgggccc tatggagatt gaatggaacg gaggagtggt
    20281 gtcgaggtac gccggggtgg tgcggcggag cacggcagac aacgccagat gctct
//
LOCUS       DASYVO010000104         4122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_102346, whole genome shotgun sequence.
ACCESSION   DASYVO010000104 DASYVO010000000
VERSION     DASYVO010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102346"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VGR03_03425"
     CDS             complement(<1..1188)
                     /locus_tag="VGR03_03425"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEV1993361.1"
                     /translation="MATRRLYYDDAFEREFTARVVYCETLPPDVNSGITGSVWSLILD
                     RTAFYPTSGGQPNDLGKIEDANVLDVRDNGDEIIHVVDRRPADPEVTGCINWPRRFDH
                     MQQHTGQHLLSAMFQERFGLPTISFHLGSEICTIDVRGPEPTEEILEGAERAANQIIF
                     EDRAVNVRYGTAEQFSQLGVRKEVNREGILRAIEIEGADLQPCGGTHVKSTGQIGVVL
                     VRRCTKVRQDWRVEFVCGGRAERSARHDFQLLRQTAEKLSCAPEDLVSAAARTLAERD
                     VNFKNLRAAQQRLAEADATISLQTTSPGANGFRVVSRVFEGVPAEYLGFFATAFTKPE
                     KTIALLAAAEGGHVHFAQHPSAGKDMSALLKQVLEKVGGKGGGARDFARGRLLDESQA
                     GKAI"
     gene            complement(1193..2398)
                     /locus_tag="VGR03_03430"
     CDS             complement(1193..2398)
                     /locus_tag="VGR03_03430"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV1993362.1"
                     /translation="MAKPLNVIELECSACGKKYEASVEQHLCTCGKPLLARYDLRRAA
                     ATLTLENLKNRPRTLWRYAEVLPNDPPVSLGEGMTALVHAERLGASMRCGHVGLQRLY
                     VKDEGLNPTGSFKARGMTTAVSRAKQLGAKVLAAPTAGNAGGALAAYAAAAGIPAVIV
                     MPADTPAANVMECQAFGAKVVKLNGLISDCGKYVAEHKDREGWYDVSTLKEPYRVEGK
                     KTMGYELWEQFGGKLPDVILYPTGGGVGLIGMCKAFDEMQEMGWIGSERPRMVAVQAE
                     GCAPIVRAWHAHQNSAQFFQNAATVASGLRVPGPLGDLMILSMLRQTNGTALTVTDEE
                     MLRAGRELASLEGIFAAPEGAATVIATRKLAASGWIKPEETVVLFNTGTGYKYAEAWQ
                     RALQSAQVE"
     gene            complement(2489..2920)
                     /locus_tag="VGR03_03435"
     CDS             complement(2489..2920)
                     /locus_tag="VGR03_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEV1993363.1"
                     /translation="MKRFFDSSVLVPVFYADHPQHASSTKLFLEAGKDDFCALRTLGE
                     VYATLTGLPLRPRITGPEGISIVKQIRERLTLITLSEQEYVSALELASSGTIVGAAAY
                     DALIGHCALKAGTDILLTWNVRDFTRLGSAIARLVKTPLEL"
     gene            complement(2917..3291)
                     /locus_tag="VGR03_03440"
     CDS             complement(2917..3291)
                     /locus_tag="VGR03_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993364.1"
                     /translation="MTLDVKPEIAAALEALASARGLSVEDYLEQLVERELPHKVEDAS
                     RAEDSASAHGMSVEDYLGELLEKELPLKLEDAARSGGSGMVWENGLFVYRTGRPLPLR
                     VVDDAIRQVREERAQHILGKHS"
     gene            3655..3849
                     /locus_tag="VGR03_03445"
     CDS             3655..3849
                     /locus_tag="VGR03_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993365.1"
                     /translation="MKRHFTAEHPWVGHVRRFSAALIAVVSIIEHLLIRIFRSGERVL
                     FHLVIFGGVVYAVYEIIKHH"
BASE COUNT          790 a         1241 c         1199 g          892 t
ORIGIN      
        1 aatggccttc ccggcctgcg actcatctaa caaacggccg cgggcgaaat cccgcgcgcc
       61 gccgcccttg ccgccaactt tttccaagac ctgtttgaga agagcgctca tgtcctttcc
      121 ggcggaagga tgctgggcaa agtgcacgtg gccgccctcc gccgcggcca agagcgcaat
      181 cgtcttttcg ggttttgtga atgcggtggc aaagaagccg aggtactcgg cgggaacacc
      241 ttcgaagaca cgggagacga cacggaagcc gttcgcgccg ggcgacgtag tctgcaagga
      301 aattgtggca tcggcctctg caaggcgttg ctgcgccgcg cgaagattct taaagttaac
      361 gtcgcgttcc gcgagcgttc gcgcggcggc agagacgagg tcttcggggg cgcagctcaa
      421 tttttcggca gtctgacgca gcaactggaa atcatgacgc gcgctgcgct cggcccgtcc
      481 gccgcagacg aactcgacac gccaatcctg gcggactttg gtgcagcggc gcaccaatac
      541 gacgccgatc tggcccgtgc tcttcacatg cgtgcctccg cagggctgca aatcggcccc
      601 ctcgatctcg atggcgcgca gaatgccctc ccgattgact tcttttcgaa caccgagctg
      661 cgaaaactgt tccgcggtgc cgtagcggac gttgaccgcg cgatcctcga agataatttg
      721 atttgcagcg cgctccgcgc cttccagaat ctcttccgtc ggttcggggc cgcgcacgtc
      781 gatggtgcag atttcggacc cgagatggaa ggaaatcgtg ggcaggccga agcgttcctg
      841 aaacatggca gacagcaggt gctggccagt gtgctgctgc atgtggtcaa agcggcgcgg
      901 ccagttgatg cagccggtca cttccggatc tgctgggcgg cggtcgacga cgtgtatgat
      961 ttcgtcgccg ttgtcgcgga cgtcgaggac attggcgtcc tcgattttgc ccaagtcgtt
     1021 cggctggcct ccggatgtgg ggtagaacgc cgtgcggtca agaatcaggc tccagacgct
     1081 gccggtgatt ccggaattca catcgggcgg gagcgtttcg caatacacaa cccgcgccgt
     1141 gaattccctt tcaaaggcat cgtcgtaata aagtctgcgc gtggccacgc cgctactcca
     1201 cctgcgcgga ctggagcgcg cgctgccagg cctcggcgta tttgtagcct gtgccggtat
     1261 tgaagagcac gacggtttct tccggtttga tccacccgga ggcggcgagc tttcttgttg
     1321 cgatcacggt tgcggcgcct tcaggagcgg cgaatatgcc ttcgagagag gcgagttctc
     1381 gtccggcgcg aagcatctct tcgtcggtca cggtcaacgc ggtgccattc gtctggcgaa
     1441 gcatgctcaa gatcatcaga tcgccgagcg gtccgggaac gcgcagaccg gatgccaccg
     1501 tcgctgcatt ctggaagaac tgagccgaat tctggtgggc gtgccacgca cgaacgattg
     1561 gcgcgcagcc ttccgcttgc acggccacca tccggggccg ctcggagccg atccagccca
     1621 tctcctgcat ctcgtcgaaa gctttgcaca tgccgatcag cccgacaccg cctccggtgg
     1681 gatacagaat aacgtccggc agctttccgc cgaactgctc ccacagttcg tatcccatcg
     1741 ttttcttgcc ctcgacgcgg tacggctcct tgagcgtgga gacgtcgtac cagccttcgc
     1801 gatccttgtg ctcggcgacg tatttgccgc agtcggagat caagccgttc agcttgacga
     1861 ccttggcgcc aaaggcctgg cattccatca cgttggctgc aggggtatct gcgggcatca
     1921 cgatgaccgc agggatccca gcggccgcgg catacgctgc gagcgcaccg ccggcattgc
     1981 cggccgtggg agcggccagt acctttgcgc cgagctgctt ggcgcgcgag acggcggtgg
     2041 tcattccgcg ggccttaaag gagccggtag gattcagtcc ttcatctttg acgtacaggc
     2101 gttgcaagcc cacatgccca caacgcattg acgcgccaag ccgctccgca tgaaccaatg
     2161 ccgtcatgcc ttcgccaagg gagactggcg gatcgttcgg caggacttcc gcatagcgcc
     2221 acagggtgcg cgggcggttc ttcagattct caagggtaag cgtggcggcg gcgcgccgca
     2281 gatcgtaacg agccaggaga ggcttgccac aagtgcagag gtgctgttct accgaagcct
     2341 cgtacttctt gccgcaggcg gagcattcca gctcaatgac attcaagggt ttggccatgt
     2401 ccgtgaaagt ctagcacagg cctttttggc tattcatgcg aggcccggcc gaaagttctg
     2461 cgaaagtcgg ggaggttcag tctcgcaact acagttccag gggcgtcttc acgagccgtg
     2521 cgatcgcaga tccgagtctt gtaaaatcgc ggacattcca ggttagcaaa atatctgttc
     2581 ccgctttcag ggcgcaatga ccaatcagcg cgtcataggc ggctgcaccg acgatagtcc
     2641 cagaggaagc tagctcaaga gcagaaacat attcctgctc actgagcgta atcagggtga
     2701 gcctttcgcg tatctgcttt acgatgctta tgccctccgg cccagtgatg cggggccgaa
     2761 gtggcaggcc agtgagcgtg gcgtagacct cgccgagtgt gcgaagcgcg caaaaatcgt
     2821 ctttccctgc ctcaaggaat aattttgtgc tagatgcgtg ctgcggatgg tcagcgtaaa
     2881 agaccggaac cagaacagag ctgtcgaaga accgcttcaa gaatgcttgc ccaggatgtg
     2941 ctgcgctctt tcttcgcgga cttggcggat agcatcatcg actacgcgca atggcagcgg
     3001 cctgccggta cggtagacaa atagcccgtt ttcccaaacc atgccactgc cccccgaacg
     3061 ggcggcatct tccagcttga gcggcagctc tttttccagg agctccccaa ggtagtcttc
     3121 gaccgacatc ccatgggcag aggcactgtc ctccgcacgg gaggcatctt ccaccttgtg
     3181 cgggagttct ctttccacga gctgctcaag gtagtcttcc acagacagcc ctctagcaga
     3241 agctagggct tccagtgcgg cggcgatttc gggttttacg tccagcgtca tcagatacct
     3301 ctgcagctca attagaagtt taagccacgc actcctctct gtcacgcgga aatgccttta
     3361 ggcctgtaac ttcgaagatt caatgggcga atttgccggg atgaaatctc tcctctcctc
     3421 cgactgtcat ctgcgctttt tgcatcccgg atggtattgc gggtgcccca tacgccgttt
     3481 ctgcgtgtgg ggcctctgac tttgattctt ccatgggtgg ccgccacctc ccaaggtaca
     3541 tcggcgagtg ggccagcctc cctttagcca aaggtattcc cggaagactt tccccgcgcc
     3601 acccgccgac ctctttggtt gacacccccc aaacttccac ttacaacatg gaacatgaag
     3661 cggcatttca ccgcagaaca tccctgggtc ggccacgtcc gccggttctc cgcggccctg
     3721 attgcagtcg tttcaattat cgaacatttg ctcattcgga ttttcagatc cggcgagcgc
     3781 gtcctctttc atctcgtcat ttttggggga gtcgtctacg cagtctatga aatcatcaaa
     3841 caccactaaa tccatcactt cgcgggtgga tcacccttaa catgctggca caactgaggg
     3901 tggcttacct tttcggtttt gattctcgaa aaggtgggct attcttcgcc ccgccttgtc
     3961 gttcgctagc ctctttgttc ttatagattt gactcgggtc ccctacggcg ggtggccttc
     4021 gataagagag aaggcgatgt caagagacac gtctccgtgg caatgctgtg aagcaccgcg
     4081 tgactgccag agtggttgag gaccgaagct ctggaactct ca
//
LOCUS       DASYVO010000105        12839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_142531, whole genome shotgun sequence.
ACCESSION   DASYVO010000105 DASYVO010000000
VERSION     DASYVO010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 12839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12839
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142531"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1114
                     /locus_tag="VGR03_03450"
     CDS             248..1114
                     /locus_tag="VGR03_03450"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1993366.1"
                     /translation="MKPAIGLIGLGLMGRPMGMNLIKAGYSLTVWNRTASRADELVAA
                     GATLAKSPQELAAASDVLLTMVSDPPALEEVLWGSNGAMQALKRGSIYMDSSTISPAL
                     ARKIAAACAECGVRFLDAPVTGGDWGAKKGELIFMIGGDADTLKDAEPILSVMGKKWF
                     HLGPHGAGQIIKLAMNLILALQVDALAEALALVTGAGLQGEKLVEVLQSSMARSGVLD
                     VKAPNLLKGEYVPSFPLRLMHKDLGLALDLANQLGVALPATAAARETYNYVKGMAKED
                     LDYSAVMKFWNK"
     gene            1171..2055
                     /locus_tag="VGR03_03455"
     CDS             1171..2055
                     /locus_tag="VGR03_03455"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1993367.1"
                     /translation="MKIGFLGLGDMGQAIVPRLLAAGHSVTGWNRTKEKAAPLFKLGM
                     LWADTPREVARKSEIVFSIVTDSAAVRSLALGENGIISGLRKDAVYLDMSTIDPDGSR
                     AVAAEFTKAGLTMMDAPVSGTTLTLSKGNASLMVGGDKAAFERVQPVLLAIGPKVTYI
                     GAQGLAVQLKIALNMTLVIEVIGFCEGIALAEKGGVPREVAVDAFLKSVVASPVLNYR
                     APLILEGHISDATYGNVNLQQKDMLLALDLGRKMGVPVPLGAAANEMLNACRGLGLAH
                     HDFVAVYEVYRRLGGMSK"
     gene            2052..2453
                     /locus_tag="VGR03_03460"
     CDS             2052..2453
                     /locus_tag="VGR03_03460"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1993368.1"
                     /translation="MNPYRTNLKDVPKVGGLSRDEGWINMQVQFLIDQKTAGSDKLLV
                     GWTVLPPGARHDKHRHPNVDEFFVVIKGSGFIYTDTGREPSGQGDVIFTPRGHWHGFD
                     NTGAEDVVLVWGWSGAGSLEAAGYEISPQGY"
     gene            2474..3196
                     /locus_tag="VGR03_03465"
     CDS             2474..3196
                     /locus_tag="VGR03_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993369.1"
                     /translation="MSAAWDDPRISRGMAAQLRHRRELLISGHKPLGWKLAFGGPAAL
                     ERLRINAPLVGFLMESAIVPSGSTLSLLNWTKPAAEPEITVHLGKDLPAGADRDTTKA
                     AIAGLGPAIEVADVDHPSDDVEGTLACDIYQRHIILGRNDPARAGGILDGLAARVLRN
                     GAEIATITDPEALQKLTGELLDNVRHTANLLAAFGESLKAGQIIIAGSIIPPIWIEPG
                     EEIMFNLAPVDTVSIRFAPAPK"
     gene            3233..4108
                     /locus_tag="VGR03_03470"
     CDS             3233..4108
                     /locus_tag="VGR03_03470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1993370.1"
                     /translation="MSAPDSARIQKFREHLHQEWTGDRTVAAWRRWHAQIAAFTQGAT
                     EAILDAAHLRPGMRVLDLASGVGDPALSIAVEVAPSGRVTATDLGPGMISLAEELARK
                     KGITNIDFREASAESLPFPGESYEVLTCRFGIMFFPDLSKALRECFRVLKPSGRAVFV
                     AWGKKEQPFFTTTAGIVLKHVPVPPPPPDPDGPSLFMFGERDRLRRALEAAGFSNVHE
                     EDRIIAGRWAGPPEEYWEQFSEVAAPFRPLLDQLTPEKMAQAKSEILAALKKFWTGKE
                     LNMPLEIVIGTATRP"
     gene            4306..5160
                     /gene="tcmP"
                     /locus_tag="VGR03_03475"
     CDS             4306..5160
                     /gene="tcmP"
                     /locus_tag="VGR03_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HEV1993371.1"
                     /translation="MIYEPSIPAKDDGLFFGEVGSWAEDKHRLVALYDSLFSTGMKKK
                     WGSRVYIDLYSGPGLLKIRDTNKFIWGSPILALDVKDPFDKYIFCENDAAALDALKKR
                     VANHFPYAKVEFVLGNCNEQVDRIGQLLPKATRTHSVLSFCFVDPFDLSIQFATIKRI
                     AEHFVDFLFLLALHMDANRNLAAYLDTKSKKIDDFLGMPNWRERWLRLQEPKSFPRFL
                     AEMYAGQMQTLRYLPVGFDKMKQVRSDVRNLPLYHLALFSRHELAYEYWDEVLNYSTP
                     QLRLDLRD"
     gene            5179..6042
                     /locus_tag="VGR03_03480"
     CDS             5179..6042
                     /locus_tag="VGR03_03480"
                     /inference="COORDINATES: protein motif:HMM:NF019146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HEV1993372.1"
                     /translation="MAEHSRIEWTEATWNPVRGCTKISPGCKHCYAETFAERFRGVKG
                     HPYEQGFDLRLVPEKLAEPLRWRTSKMIFVNSMSDLFHDEVPDNYIVAVAQVMVAAKW
                     HTYQVLTKRSERLRKLLNTKLRFAAEQRHIWWGVSVEDKKYGLPRMEDLRSANAGVRF
                     LSVEPLLEDMGKLDLRGIDWMIVGGESGAGARPMKKEWALPLRDQCKRSGVPFFFKQW
                     GGVHKAKAGRRLEGRTYDEFPSRVRHPILSAEDCKYAASEIEARLYSGSVIPLSALGT
                     QGRSALAVRVA"
     gene            6638..7594
                     /locus_tag="VGR03_03485"
     CDS             6638..7594
                     /locus_tag="VGR03_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1993373.1"
                     /translation="MSKAIRTWTVFAMVGAALVCGQAAAAQQEPDWSKVQIKVTKVSG
                     NIYMLEGQGGNIAASVGEDGIVIVDDQFAPLADKIQAALKELKITDKPVRFVINTHFH
                     FDHTGGNLPFNNAGATLIAQDNVRKRLEAGGTFGNGGSIKMDAKPAPKAALPIITFEH
                     DVTVHLNGEDIRALHFPSGHTDGDSIIFFPKNNVVHMGDDFVRYGFPFIDVASGGSVQ
                     GMIDAMEKATAQLPPDVKVIPGHGDLSNLDDVRAFTKMLKETSAVVQKAINDHKTLDQ
                     MKQEKILAPWAKWNGDFVNDDAFIETLYNSLTGHKGEFVKHN"
     gene            complement(7722..8738)
                     /locus_tag="VGR03_03490"
     CDS             complement(7722..8738)
                     /locus_tag="VGR03_03490"
                     /inference="COORDINATES: protein motif:HMM:NF040257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993374.1"
                     /translation="MKKKIRAIQYGVGPIGASIARLMREKQAIEIIGAIDTDPAKVGR
                     DLGEVVGAKDAPWGVTVSAEAKEVLEQAADVVIHSTSSSLEKVMDQLLLCLEAESCIV
                     STCEELSYPFRSHPELAAKLDTAAKEWGVALVGTGVNPGFVMDKLLVTLAAVSQQIDH
                     AKALRIVDASKRRLPLQNKIGAGMTVEEFRAQVKAGVIKHVGLPESVAMVADSLNLPV
                     EQIIETIEAKVATERVQTEYLTVEPGQAAGVHQIARGLSKDGKELVYLELQMYVGAKD
                     PADTLSLTGHPNISLTIHGGSPGDIATASVIVNSIPAILDASAGLRTSRDLPLGFFSP
                     NARP"
     gene            8959..9249
                     /locus_tag="VGR03_03495"
     CDS             8959..9249
                     /locus_tag="VGR03_03495"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEV1993375.1"
                     /translation="MPAKKLSEAEITTLLAKTKGWNLVNGKLHREFTCNNFVTAFGNM
                     TRVALVAEGMNHHPEWSNVWNKVVIDLNTHSVKGISDYDFTLAEKINEIFGA"
     gene            9280..10260
                     /locus_tag="VGR03_03500"
     CDS             9280..10260
                     /locus_tag="VGR03_03500"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEV1993376.1"
                     /translation="MSADRQPQKSSWSWIFLVARWTAAMLILGLLLYLVPVAPLRNAL
                     SHVPLSRFIAVLLIYLVALTGGITKWHTVVNSAGAQLSFAASAQCYASGLFGALFLPS
                     IIGGDVARLAVGISHSPRPAAVITGNVADRFLDVAAQLTLVSLGLLLLPGSLPVPLQG
                     PAKRVLFAGVLAAAILFGLVVALRRPLLRGRSIRFRRRLAQLRHAIRAVSRRPGRLVF
                     GWLLGTSVQGTYVLLTALLGITCGLGLPLRVWLFAWPLAKIAAVMPITQGGIGVREAA
                     LVVLLAPFGAPASQVLATGIVWEGVIITGGLLAGLTAFLLRLAESRPQGS"
     gene            10499..11410
                     /locus_tag="VGR03_03505"
     CDS             10499..11410
                     /locus_tag="VGR03_03505"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEV1993377.1"
                     /translation="MNEKKLRILLAEGDPGETASGLRALYPEGQDGLELNNVSSVATL
                     IATLEIVSPEVIFLDLSLAHPDPLDAVRRVHRSAPAVPLIVLADGSDKNCAVRSLSQG
                     ALNYLIKGFIDFQTLERVLRAALEHNTLEGLADLLRDSLTGLYIRDGFLTLGERAMET
                     AKRNASTLVLLNMRIENLEALRAGYGSTAVEKSLRGVATLLAGSFRRTDIVARLAESQ
                     FAALAVDAAEPSAPVLCQRLEKRIAVLNRDMGPWGPLELRMNARFWSPRETTTFSEFL
                     DSVEAGLRSAPVLPVEGPARRDSVSRK"
     gene            11416..>12839
                     /locus_tag="VGR03_03510"
     CDS             11416..>12839
                     /locus_tag="VGR03_03510"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEV1993378.1"
                     /translation="MDSASKFWRRLITVGILINTLLFLDLYFAIQSTGQAEGMNYRVA
                     HTQEVLGVIAHARLERLRLQLQAWNYGSTRNPHFLEQFQSDLHGLRGNLDRLRILTVD
                     DSAQQKILNQLVPGAMAQAYSLESGMQPAPSSPPSSSSAPFGLPEDASSEENLAQLFD
                     ALEDNELALLVSRSEAVQRNARQTRLVLFLAGILTFAVLCAGGFLIRRELKMRSEIES
                     GLQRARELLGGKYEEGRAELGQALSNLHEQIGARQLAEDEICRLNKDLEHRVELRTAE
                     LKEANRELEAFSYSVSHDLRAPLRHLDGFSRILQQEYGPQLPEEAVHYLERIRSAASK
                     MAALVEDLLHLSRIGRQLPHLETLALDSLVAEAQAEVLPEAGDRSIHWQIHSLTEVDA
                     DSVLLRQVFTNIFSNAIKFTRRQPRPVIEVGSRQECAETVIFVRDNGVGFDPRYADKL
                     FGVFQRLHRQDEFEGTGIGLATVQR"
BASE COUNT         2640 a         4028 c         3492 g         2679 t
ORIGIN      
        1 accctcaaag ccgcccctga ggctcgtgtc acgtccaagt ccagcgataa gcctctcagc
       61 gccgccgcca ccgcctcggc cggcggtgcc cccggcctcc tccccctccc ccaccgtccc
      121 aagaccgcct gatgcgcaca ccaacttccc ctcacagctt cctcccatgg attcggctac
      181 aatcgtgtcc tcttttaatg gaaaataccg gcgcgcattg cgtctgccgt tttcgaggag
      241 gctcttcttg aaacctgcca ttggcttgat cggtctcggc ctcatgggtc gtcccatggg
      301 catgaatctc atcaaagccg gctactctct caccgtttgg aaccgcactg cttcgcgcgc
      361 cgatgaactc gttgccgccg gcgccacgct cgcaaaatcc ccgcaggaac tcgccgccgc
      421 ttccgatgtt cttctcacca tggtcagcga tcctcctgcc ctcgaagaag ttctctgggg
      481 ctcgaatggc gcgatgcagg cgctcaagcg cggcagcatc tacatggact ccagcacgat
      541 ctctcccgcc ctcgcgcgca agattgccgc cgcctgcgcg gaatgcggcg tgcgctttct
      601 cgacgcccct gtcaccggcg gtgactgggg cgccaagaaa ggtgagctga ttttcatgat
      661 cggcggcgac gccgatacgc taaaggacgc cgagcccatc ctcagcgtga tgggcaagaa
      721 gtggtttcac ctcggccctc atggtgctgg ccaaatcatc aagctcgcga tgaatctcat
      781 cctagcgcta caggttgacg cccttgccga agctctggct cttgtcaccg gcgccggtct
      841 tcagggcgaa aaactcgtcg aagtcctgca atccagcatg gcgcgctccg gcgttctcga
      901 cgtcaaggcc cccaatctcc tcaaaggcga gtacgtcccg agtttccctc tgcgcctcat
      961 gcacaaggat ctcggcctcg cgctcgacct cgccaatcag cttggtgtcg ccttgccggc
     1021 caccgccgcc gctcgcgaaa cctacaacta cgtcaaaggc atggccaagg aagacctcga
     1081 ctactccgcc gtcatgaaat tctggaataa gtgacgcacc cgcatcgcta gaattaccct
     1141 gcatttttcg agtcgagaaa gaggtgcttc gtgaaaatcg gtttcctcgg tcttggcgac
     1201 atgggccagg ccatcgttcc gcgcttgctt gccgctggcc actccgtcac cggctggaac
     1261 cgcaccaaag aaaaagccgc gcccctgttc aagctcggca tgctttgggc cgacacgccc
     1321 cgcgaagtcg cccgcaaatc ggaaatcgtt ttctccatcg tcaccgactc cgctgcggtc
     1381 cgctccctcg ccctcggtga aaacggcatc atctccggcc tgcgcaaaga cgccgtttac
     1441 ctggacatga gcaccatcga ccccgacggc agccgcgccg tcgccgccga attcaccaaa
     1501 gccggcctga cgatgatgga tgctcccgtc tccggcacca cgctcacgct ttcgaaaggc
     1561 aacgcctcgc tcatggtcgg cggcgacaag gccgcattcg aacgcgtcca gccggtcctc
     1621 ctcgccatcg gcccgaaagt cacctacatc ggcgcgcaag gcttggccgt gcagctaaaa
     1681 attgcgctca acatgacgct ggtcatcgag gtcatcggct tttgcgaagg catcgcgctc
     1741 gcggaaaaag gtggcgtccc ccgcgaagtc gccgtcgacg cttttctgaa gagtgtcgtc
     1801 gcctctcctg tgctcaacta tcgcgcgcca ctcattctcg aaggacacat ttccgacgcc
     1861 acttacggca acgtcaatct ccagcaaaaa gacatgctgc tcgcgctcga tcttggccgg
     1921 aagatgggtg tccccgtccc gctcggtgcc gccgccaacg aaatgttgaa cgcctgccgc
     1981 ggcctcggcc tcgcgcatca cgattttgtc gccgtctacg aggtctaccg ccgcctggga
     2041 ggcatgtcga aatgaatccc taccgcacca atctcaaaga tgtccccaaa gtcggcgggc
     2101 tctcgcgcga cgagggctgg atcaacatgc aggtccagtt tctgatcgat caaaagactg
     2161 ccggctcgga caaacttctc gtcggctgga ccgttcttcc ccccggcgcg cgccacgaca
     2221 agcatcgcca tcccaacgtc gatgaatttt tcgtcgtgat caaaggaagc ggattcattt
     2281 acacggacac cgggcgcgag ccgtctggcc agggcgatgt gattttcacg ccgcgcggcc
     2341 actggcacgg tttcgacaac accggcgccg aagacgttgt cctcgtctgg ggctggagcg
     2401 gcgcgggctc gctcgaagcc gccggctacg aaatttcgcc ccaaggctac tgatcctttg
     2461 tggcttccca atcatgagcg ccgcctggga cgacccgcgc atttcccgcg gaatggccgc
     2521 gcaacttcgc catcgccgcg aacttctcat ctccggccac aaacctctcg gctggaaact
     2581 tgccttcggc ggccccgcag ccctcgagcg gctccgcatc aacgcccccc tcgtcggttt
     2641 cctcatggaa agcgctattg ttccttccgg ttcaacgctt tctcttttga attggacgaa
     2701 gcccgcagcc gagcccgaaa tcaccgtgca tcttggcaag gacttgcccg cgggcgcgga
     2761 ccgcgacacc acaaaagccg ccatcgccgg actaggccct gccattgaag ttgccgacgt
     2821 cgatcatccc tccgacgacg ttgaaggcac gctggcctgc gacatctatc agcgccatat
     2881 catcctcggc cgcaatgatc ccgcgcgagc cggcggcatt ctcgacggcc tcgccgcccg
     2941 cgtcctccgc aacggagccg aaatcgccac catcaccgat cccgaggctc tgcaaaaatt
     3001 gaccggcgaa ctcctcgaca acgttcgtca cactgccaac ttgcttgcgg ctttcggcga
     3061 atctctcaaa gccggtcaaa tcatcatcgc cggttccatc atcccgccca tctggatcga
     3121 gcccggcgaa gaaatcatgt tcaacctcgc gccggtggat acggtctcca tccgcttcgc
     3181 tccggcaccc aaatgaacac tctgctaaac ttccccgtcg tggaggccat tcatgagcgc
     3241 gcctgattcc gcaagaatcc agaagttccg cgagcatctt caccaggaat ggaccggcga
     3301 caggaccgtc gccgcctggc gcagatggca cgcgcagatt gccgcgttca cgcagggcgc
     3361 caccgaagcc atcctcgatg ccgcccatct tcgccccggc atgcgcgtcc tcgatctcgc
     3421 cagcggcgtc ggcgatcccg cgctttcgat tgccgtggaa gtggctccct ccggccgcgt
     3481 gaccgccacc gatctcggcc caggtatgat ctcgctcgcc gaagaactcg cgcgcaagaa
     3541 aggcatcacc aacatcgatt tccgcgaagc cagcgccgag tctctcccgt tccccggcga
     3601 atcctacgaa gtcctcacct gccgcttcgg aatcatgttt tttcccgatc tctccaaagc
     3661 gctccgcgaa tgttttcgcg tattaaaacc cagcggccgt gccgtgttcg tcgcttgggg
     3721 caagaaagag cagcccttct tcaccaccac ggcaggcatc gttctgaaac acgttccggt
     3781 gcccccgccg ccacccgatc ccgatggccc cagcctcttc atgttcggcg agcgcgaccg
     3841 cctccgtcgc gcgctggaag cggccggctt cagcaatgtc cacgaggaag atcgcatcat
     3901 cgccggccgt tgggccggac cgccggaaga atactgggag cagttcagcg aggtcgccgc
     3961 gcccttccgc ccgctcctcg atcagctcac ccccgaaaaa atggcgcaag ccaaatccga
     4021 aatcctcgcc gccctcaaaa aattctggac cggcaaggaa ctgaacatgc ccctcgaaat
     4081 cgtcatcggc accgccacgc gcccttaact ctcgcaccta cagcagccgc ctccactcgt
     4141 gtgtagcggc aggttcattt cgccgtcatg tctttcgctc tcagttgcgg attgagaact
     4201 gccagcgatg caattcatca agcgcaaaat aagcagaatt tctttataac gctccgaata
     4261 ttatccgagt gttctaaatt gcatatgtgt tacgtttaag ccgctttgat ttacgagccc
     4321 tcaattccgg ccaaagatga tggacttttc ttcggcgaag taggcagttg ggccgaggac
     4381 aaacaccgcc ttgtcgctct ctacgatagt ttgttctcca ccggcatgaa gaaaaaatgg
     4441 ggctcgcgcg tgtatatcga tttgtactca ggcccgggtc ttctgaagat tcgcgatacg
     4501 aacaagttca tctgggggtc accgatcctc gctctggacg taaaagaccc cttcgacaaa
     4561 tacatttttt gcgaaaatga cgccgcggcg ctggacgcgt tgaagaagcg tgtcgcgaat
     4621 cactttccat atgccaaagt agaattcgtc ctgggaaact gtaatgagca ggtcgacaga
     4681 ataggtcagt tgcttccgaa ggccaccaga actcatagcg ttctgtcttt ttgctttgtc
     4741 gatcccttcg acctttcaat ccaattcgcg acgataaaga gaattgcgga gcatttcgtt
     4801 gacttcctat tcctcctggc gcttcacatg gacgcaaacc gtaatcttgc tgcttatctt
     4861 gatacaaaga gtaaaaagat cgacgatttt cttggcatgc ctaattggcg tgaaagatgg
     4921 ctgcgacttc aggagccaaa gagttttccg cggttccttg cggagatgta tgctggacaa
     4981 atgcaaacgc tccggtacct acctgtagga ttcgacaaaa tgaaacaggt acgttccgac
     5041 gtgaggaatc ttcccctata tcacctcgct ctattttcaa gacacgaact cgcatacgag
     5101 tactgggatg aagtactgaa ttacagcact ccacagctac gattggattt gagagactaa
     5161 aattgctgag gcgcgagaat ggctgagcac tccagaatcg aatggacaga agcaacctgg
     5221 aacccggtcc gtggctgcac gaagatcagt cccggctgta aacactgtta cgcggaaacc
     5281 ttcgcggagc gcttcagagg tgtgaaaggc cacccgtatg agcagggctt cgatctgcgg
     5341 ctagtcccag agaaacttgc ggaaccgctc cgttggcgta catcgaaaat gatcttcgtc
     5401 aattccatga gcgacctttt tcacgacgag gttccagaca attacatcgt agcagtagca
     5461 caagtaatgg tggcggccaa gtggcatacc taccaggtgc tgaccaaacg gtccgaacgc
     5521 ctccgcaaac ttctcaatac aaaacttcgt ttcgctgccg agcagagaca catctggtgg
     5581 ggagtaagcg tggaagacaa gaaatatggt ttgccgcgca tggaagactt gcgaagcgca
     5641 aacgcgggag ttcgctttct ctccgtcgag ccattgttgg aggacatggg caagctggac
     5701 ttgcgaggca tcgactggat gatcgtcggt ggtgagagcg gcgccggtgc ccggcccatg
     5761 aagaaagaat gggccctgcc ccttcgcgat cagtgcaagc gcagcggtgt tcccttcttc
     5821 ttcaaacagt ggggcggagt tcacaaggcc aaggcgggca gacgcctcga aggcaggacg
     5881 tatgacgaat tcccaagccg cgttcgtcac ccaattctgt ccgcggaaga ctgcaagtac
     5941 gcggcaagtg aaatcgaagc acgcttatat tctggaagcg taattcctct gtctgcgctt
     6001 ggtacgcaag gtcgcagtgc gctcgctgtg agagtggctt aggcacgacc gagccgcgct
     6061 atcccccctc gaatcaccaa cttccagccc caatctcatt cgaatgatag ccacaacgta
     6121 atattcatgg acagttagcc agaagtgtgc tacgctgttg catgaactgg acgctagcca
     6181 gcccggtcgg acttcaccgc gcgctcttcg cagcatcccc gaaaacggaa gcagtttttt
     6241 ctgcaccatt ttggtgcagt gcaagtcctt tagatgcaac acttccgggc attctcgtat
     6301 gtgttgcatc taaagggctt agaggaattg taagtctttt agatgcaaca cttgcaaaaa
     6361 caggggggta gggtctgtta tggttaacca ggaatccgat aaggattcct gtcctgagga
     6421 gcatcgcgac gaccgaaggt tccggccggg tcggaaagga tctctcttcc caggcgacga
     6481 gcgttgtttg cgagtcccga tccatcggca ccatcgagag tcgaccgaag gttccgacct
     6541 tgttggaagc gatttctcct cgaatccgat cctgaccatc cacctgggcg ctgtccacaa
     6601 tgatagaatc ccccgcgcat tcctgaggag gagcgagatg agcaaagcga taaggacttg
     6661 gacggttttt gcaatggttg gcgcggcgct ggtttgcgga caggccgcag cggcacagca
     6721 ggagccggac tggtccaagg tccaaatcaa agtgaccaaa gtttccggca acatctacat
     6781 gctggaaggc caaggcggca acatcgccgc ttccgtgggc gaagacggta tcgtcatcgt
     6841 ggacgaccag ttcgctccgc tggcggataa aatccaggcg gcgctgaagg aattgaaaat
     6901 tacggacaag cccgtgcgct tcgtcatcaa tacccacttt cacttcgatc acaccggcgg
     6961 aaacttgccg ttcaacaacg caggagccac gctcatcgcg caggacaatg tccgcaaacg
     7021 gctcgaagcc ggcggtacgt tcggaaacgg cggctcgatc aaaatggacg cgaagcctgc
     7081 gcccaaggcc gctctcccca tcatcacatt tgagcacgac gtcaccgtgc acctgaacgg
     7141 agaagatatc cgcgcgctgc atttcccctc cggccacacc gacggcgact ccatcatctt
     7201 cttcccgaaa aataatgtcg tgcacatggg cgacgacttc gtgcgttacg gcttcccgtt
     7261 tattgacgtg gccagcggcg gcagcgtgca gggcatgatc gacgccatgg aaaaagctac
     7321 cgcgcagttg ccgccggacg tgaaggtcat ccccggccac ggcgacctct ccaaccttga
     7381 cgacgtccgc gccttcacca aaatgttgaa agagacttcg gccgtcgtgc agaaagccat
     7441 caacgatcac aagaccctgg accaaatgaa gcaggagaaa attctcgcgc cgtgggcaaa
     7501 atggaacggc gatttcgtca acgatgacgc ctttatcgaa acactctaca attcgctcac
     7561 cggccacaaa ggagaattcg taaagcacaa ctgagaagtt tagttgttat ttggttttga
     7621 atttgtgtag ggccgggctt caggccggcc tgttttcaaa gccagctgac ggccgcccat
     7681 tagaaacgta ccgagtcgtt tatctgtttt ccagtttcgt ttcagggccg cgcattcggc
     7741 gagaagaagc caagcggcaa gtcgcgcgaa gtgcgcaggc cagccgaggc atccaggatc
     7801 gccgggatcg agttcacgat cactgacgcc gtggcgatat cgccgggcga gccaccatga
     7861 atcgtcaaac tgatatttgg atgcccggtc agcgagagcg tgtcggcagg atctttcgcg
     7921 ccgacataca tttgcagttc gaggtacaca agttctttac cgtctttcga gaggccgcga
     7981 gcaatctggt gtacgcccgc cgcctggccc ggctcgaccg tcaggtattc ggtctgcacc
     8041 cgctcggtgg caactttcgc ctcgatcgtt tcgataattt gctcgacggg caaattcaag
     8101 ctgtccgcca ccatcgccac cgactccggc aaccccacgt gcttgatcac gcccgctttc
     8161 acctgcgcgc gaaattcttc aaccgtcatt cccgccccga ttttgttctg cagcggcaac
     8221 cgccgcttcg acgcatcgac gatgcgcaaa gctttcgcat ggtcgatctg ctgcgacacc
     8281 gccgccagcg tcacgagcag cttgtccatg acgaaaccgg gatttacgcc cgtaccgacc
     8341 agcgccacgc cccactcctt cgccgccgta tccaatttcg ccgcgagttc cggatgcgag
     8401 cggaacggat acgacaattc ttcgcaagtc gaaactatgc acgactccgc ttccaggcac
     8461 agtagcaact ggtccatcac tttctcgagc gacgacgacg tcgaatggat caccacgtcc
     8521 gcggcctgct ccagcacttc cttggcttcc gcggaaacgg taacgcccca cggcgcatct
     8581 tttgcgccga ccacttctcc caggtcgcgc ccgaccttcg ctggatcggt atcgatggcg
     8641 ccgatgattt caatggcctg tttttcccgc atcaggcgcg caatggatgc gccgatcggg
     8701 cccacaccat actggatggc gcgaattttc tttttcacgt cgctctccgc tttccgtttg
     8761 catgcgcgca caacgcgcat ttcggcttca aaggaatccc ttacaatagt ggatttcccg
     8821 cgcgcattca acgaagaacc gcatcaagcg tttacaccgt ctgcagctcg tgccagcgaa
     8881 gccggatttg ttgcaggcgc agggcttgcg ccgcataatg gctggagaga acgccagccg
     8941 attccggagg atgacgacat gcctgcaaaa aaactttccg aagccgagat tacaacgctg
     9001 cttgccaaga cgaaaggctg gaatcttgtc aacggcaagc tccaccgcga gtttacctgc
     9061 aataatttcg tcactgcttt cggcaacatg acccgcgtcg cgctagtggc cgaagggatg
     9121 aaccaccatc ccgaatggtc caacgtgtgg aacaaggtgg tgatcgacct caatacgcac
     9181 agcgtgaaag gcatcagcga ctacgacttc acgctggcgg aaaagatcaa cgaaatcttc
     9241 ggcgcgtgat tcgttgccat agaaaacgat cttccgataa tgagcgcaga ccgccagccg
     9301 caaaaatcca gctggagttg gatttttctt gtagcgcggt ggacggcggc gatgttgatc
     9361 cttggtcttc tcttgtatct ggtaccggtg gcgccgctgc gaaacgcgtt gtcgcacgta
     9421 ccgttgtcac gttttattgc cgtacttctg atttatctgg tggctttgac cgggggcatc
     9481 accaagtggc acacggtggt gaattccgca ggcgcgcaac ttagcttcgc agccagcgcg
     9541 cagtgctacg cgagcgggct attcggcgcg ctctttctgc catcgattat cggcggcgac
     9601 gtcgcgcgcc tcgccgtggg aatttcgcac agtccgcgtc ctgccgcggt gatcaccgga
     9661 aatgtggcgg accgttttct ggatgtcgcc gcgcagctca cacttgtctc gctgggcctg
     9721 ctgctgctgc cgggttcctt gcccgtgccg ttgcaggggc cggcgaagcg cgtgctcttt
     9781 gccggcgtcc ttgccgcggc aattcttttc ggtctcgttg tggccttgcg caggccgctc
     9841 ttgcgcggac gctccattcg tttccgccgc aggctcgcgc agcttcgtca cgcgattcgc
     9901 gcggtttcgc gccgccccgg gcgactggtt tttggctggt tgctgggaac ttccgtgcag
     9961 ggcacgtatg tcttgctcac cgcactcctg ggaattactt gtggcctcgg tctgccgcta
    10021 cgtgtgtggc tcttcgcgtg gccactggcc aaaatcgccg cggtcatgcc cattacgcaa
    10081 ggtggaattg gggttcgcga ggcggcgctg gtggtactgc tcgcgccgtt tggtgcaccg
    10141 gcgtcgcagg ttcttgcaac cggcatcgtt tgggagggcg tgatcatcac cggaggtctt
    10201 ctcgcaggac taacggcgtt tctcttgcgg ttggcggagt cgcgcccgca gggctcatag
    10261 cgtttccgtc actcccctcg aagtttgctt gactcctacc gccgctgcgg caaaatggac
    10321 gaaccccctt ccaagcagtt gattgtccag aggactgccc tttccagtct cgtgaattga
    10381 gcgggcttgc gatgacttgt cgcatcaggt ggattgacac cggattcgaa tcgatcgccg
    10441 ttcttgccgc aggcgatcct ttttctctgc gagcctcagc ggaagcgagg caacacgcat
    10501 gaatgaaaag aaattgcgca tattacttgc ggaaggagat cccggtgaga ccgcttccgg
    10561 tttgcgagcg ctctacccgg agggccaaga cggtctggaa ttgaacaacg tttcctcggt
    10621 cgcgacgctg atcgccacac tcgaaatcgt aagtccagag gtcatcttcc tcgatctgtc
    10681 gctcgcgcat cccgatccgc tggacgcggt gcgccgcgtg caccgctcgg cgcccgcggt
    10741 gcctctgatc gtgctcgccg acggcagcga taagaattgc gccgtacgaa gcctgagcca
    10801 gggggccctg aattatctca tcaaaggatt catcgacttc cagacactcg aacgtgttct
    10861 gcgcgcggct ctggagcaca acacactcga gggcctggcc gatctcctgc gggattccct
    10921 cacgggactc tacatccgcg atggttttct cacgctcggg gagcgcgcga tggaaacggc
    10981 gaagcgcaac gcgagcactc tcgtcttgct gaacatgaga atcgaaaatc tggaggcgct
    11041 tcgcgcggga tatggttcca ccgccgtcga gaaatccttg cgtggggtag ccacgctcct
    11101 agccggcagt ttccgccgga cggacatcgt ggcccgtctt gccgagtcgc aattcgcggc
    11161 actcgccgtc gacgcggcgg agcccagcgc accggtgctc tgccagcggc tggaaaagcg
    11221 catcgccgtg ctcaatcgcg acatggggcc gtggggcccg cttgaactcc gcatgaatgc
    11281 gcggttctgg tctcccaggg agaccaccac attttctgaa ttcttggaca gcgtcgaggc
    11341 ggggttgcgg tccgcgccgg tactccccgt agaaggcccg gcgagacgcg actccgtatc
    11401 ccgaaagtga agaagatgga tagcgcatcc aagttctggc gaaggctcat cacggtggga
    11461 attctgatca ataccctcct gttcctggat ctatatttcg ccattcagag taccggccag
    11521 gcggagggta tgaactaccg ggtggctcat acccaagagg tcctcggcgt catcgcgcat
    11581 gcccgactgg aacgcttgcg cctgcaactc caggcttgga actatggttc gacgcgcaac
    11641 ccgcactttc tggagcaatt tcaatccgat cttcatggat tacggggcaa tctcgatcgc
    11701 ttgcggatcc taactgttga cgactccgcg cagcagaaga ttctcaatca actcgtccct
    11761 ggcgcgatgg cacaagccta ttcgctggaa agtggaatgc agccggcgcc atcttcgcct
    11821 ccatccagct ccagtgcacc attcggtttg cctgaggatg catcctcaga ggagaacctc
    11881 gcgcagctat tcgacgcact ggaagacaac gagcttgcgc ttttagttag caggtctgaa
    11941 gccgtgcaaa ggaacgcgcg ccagactcga ttggtacttt tcttggcggg aatactcact
    12001 ttcgccgtct tgtgtgcggg cggcttcctc attcgtcgcg aattgaagat gcgctccgaa
    12061 atcgaatcag gcctgcaacg ggcgcgggag ctcctgggag gaaaatatga agaaggacgc
    12121 gccgagctcg gccaggctct aagcaatttg cacgagcaga ttggcgcacg gcagctggcc
    12181 gaagacgaga tctgccggct gaacaaggat ctggagcacc gggtggagct gcgcaccgcg
    12241 gagctcaagg aagcaaaccg cgagcttgaa gccttttctt attccgtatc gcacgatctg
    12301 cgagcaccac tgcgccacct ggacggcttt tcccggatcc tccagcagga atacgggccg
    12361 caacttccgg aggaggccgt gcactatctc gagcgcatcc gctccgcggc gtcaaagatg
    12421 gccgccctgg tcgaagatct actgcatctc tcccgcatcg gacgccagtt gcctcatctt
    12481 gaaacgctag cgctggactc gcttgtcgca gaagctcagg cggaagtgct ccccgaagcc
    12541 ggagaccgtt caatccactg gcaaatccat tcgctcactg aagtcgatgc cgattccgta
    12601 cttctccgcc aggttttcac caatatcttt tccaatgcca tcaaattcac ccgccgacag
    12661 cctcgtcccg tgattgaagt cggcagccgc caggaatgcg ccgagaccgt aattttcgtt
    12721 cgcgacaacg gcgtgggttt cgatccgcga tacgccgaca aactcttcgg cgtttttcag
    12781 cgcctccatc ggcaggacga attcgaaggc acgggcattg gcctagccac ggtgcagcg
//
LOCUS       DASYVO010000106         7966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_171734, whole genome shotgun sequence.
ACCESSION   DASYVO010000106 DASYVO010000000
VERSION     DASYVO010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7966
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171734"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /gene="eda"
                     /locus_tag="VGR03_03515"
     CDS             complement(<1..579)
                     /gene="eda"
                     /locus_tag="VGR03_03515"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV1993379.1"
                     /translation="MDKQKIRDRIIEIGIVPVVRAASPREARIATDAVCEGGIPIVEI
                     TMTVPGAVDVIRELTKGGASDVLVGAGTVLNLEAARRCLDAGAQFLVSPGLNLQVVEL
                     AVREGKLMMAGALTPTEVITAWEAGSDFVKVFPCGMVGGAKYIKALKGPLPQVPLVPT
                     GGVNLNTAAEFIEAGAAALGIGGELVQADALRA"
     gene            complement(608..1702)
                     /locus_tag="VGR03_03520"
     CDS             complement(608..1702)
                     /locus_tag="VGR03_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV1993380.1"
                     /translation="MGLTIKPKKDCRWDLISLGEVMLRLDPGERRIAAARSFEVCEGG
                     GEYNVARGLKRCFGLDTAVVTAFADDPVGRLLQDLIYQGGVDQSLIRWVEHDGVGRAA
                     RNGLNFTERGFGVRAALGSYDRGHTAVSQLKPGEIDWDQIFGKQGARWFHTGGIFCAL
                     SQTTPEVTLEAMQAARRHGTVVSYDLNYRASLWKSHGGKKQAQEVNRRIAPHVDVMLG
                     NEEDFSAALGYEVPGMDEHHSTFEVEGFKQMIAKAVKDYPFKVVATTLRKAKTATLND
                     WGAICYCDGKFCQARNRENIEIFDRVGGGDSFASGLIYGFLAGQDPQWSVECGAAHGA
                     LAMTTPGDTSMATLQEVLQVMKGVGARIAR"
     gene            complement(1797..3077)
                     /locus_tag="VGR03_03525"
     CDS             complement(1797..3077)
                     /locus_tag="VGR03_03525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1993381.1"
                     /translation="MASERTLPTAPSRPGYFRWVICGLLLLGTTKNYMDRWVLSALKT
                     TLQHDLGWNEINYSDLVVAFQAAYAVGMLAVGRFIDRFGTRLGYAIIMVFWSLASMAH
                     AVGSSFSSFLMARVALGFGESGVFPASIKAVAEWFPAKERALATGIFNAGTNMGVILA
                     GLSVPWITAHFGWRWAFVIVGSLGFAWLALWLLVYQRPEDHPRCSKAELEYIRGDMPQ
                     PTQKIKWLSLLPYRQTWAFACGKFLIDPIWWFYLFWVPDFLERRYGLHLAQIGLPVVV
                     IYLISDVGSVAGGWLSSFLIRRGVSVNAGRKIAMLLCAITVVPIVSAYHVSGLWPAVL
                     LIGLAAAGHQGFSANLYTLTSDMFPSRAVASVVGIGGMAGAVGGMLIAKIVGHVLQWT
                     GSYMVPFFIAGSVYLVALAAIHVLAPRLEPAKIN"
     gene            complement(3254..4462)
                     /gene="manD"
                     /locus_tag="VGR03_03530"
     CDS             complement(3254..4462)
                     /gene="manD"
                     /locus_tag="VGR03_03530"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF011654.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HEV1993382.1"
                     /translation="MKITDAKVIVCSPGRNFVTLKITTEDGIYGLGDATLNGRELAVA
                     SYLSDHLIPLLMGRDARRIEDTWQYLYKGAYWRRGPVTMSAIAAVDTALWDIKGKALN
                     VPVYQLLGGASRDGVLVYGHASGQDIEETVQAVADYLKLGYRAIRAQSGIPGLQSTYG
                     VGRGKMHYEPAEKDAPVENAWSSELYLNFVPRLFERLRKEFGADLHLLHDAHHRLTPI
                     EAGRAGKSLEPYHLFWLEDPVPGELQESFRIIRQHTTTPLAVGEVFNSIYDCQQLIQE
                     QLIDYIRTTVVHAGGISHLRKIASLAEHYHVRTGSHGATDLSPVCMAAALHFDLSVHN
                     FGIQEYMRHADETDRVFPHSYTFSGGMMHPGDAPGLGVDIDEELAAKYPYRRAYLPIN
                     RKLDGTMHSW"
     gene            complement(4645..7599)
                     /locus_tag="VGR03_03535"
     CDS             complement(4645..7599)
                     /locus_tag="VGR03_03535"
                     /inference="COORDINATES: protein motif:HMM:NF027309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HEV1993383.1"
                     /translation="MIRDPLRRHPSMEDWAIRCTRSVGTAGLFLIFYLLASPLLHALT
                     IGEDRYVDPAYHAGDFSIVTRNAAATLFVEADDFPGVVRAVNDLQADIERVSNCTTAI
                     AHDGKNLGPNMIIVGTVGKSKIIDQLIRDRKIDVTSIARKWESFLIQVVPKPLPGVAS
                     ALIIAGSDKRGTIYGVYDLSGQIGVSPWYWWADVPVQHHDELFVKPGIHVQGPPAVKY
                     RGIFLNDEAPSLSGWVKGKYGNYNHEFYTKVFELLLRLKANYLWPAMWNNAFNEDDPL
                     NPQLADEYGIVMGTSHHEPMLRAQQEWKRHGKGPWDYSKNAEVLKEFWDQGVERNKKY
                     ESIITLAMRGDGDLPMSESANIALLEKIVADQRKMIADRVNPDLSKVPQDWALYKEVQ
                     EYYEKGMRVPDDVTLLWCDDNWGNIRRLPTQEERKRSGGAGVYYHFDYVGDPRSYKWL
                     NTIPIAKVWEQMNLAYHYGADRIWIVNVGDLKPMELPIDFFLNMAWKPENWPKEKISE
                     FTRLWAERQFGREFAPEIADILSKYTKYNGRRKPELLEPGTFSLVDYREADTVFADFQ
                     AAAAKAEEIYGKLPENQRDAFFELVLYPTKASAIVTELYITAGRNNLYASQGRASAND
                     MAAEARALFQADADLSAYYNHTLAHGKWDHMMDQTHIGYTFWNQPPVNVMPKVTEIEI
                     PAPAQLGVAVEGSTLAWPGSPGEPTLPPFDLFNQQRRYIDIFNRGREPFVFSATSSVP
                     WIVLSESQGTIEKERRVWVSVDWNKAPVGSTSGSVKIAGGSAQEVVVKVYASRPQDLT
                     RESLDGFVESDGYVSMEAVHYGKKVDGTSARWERIDDLGRTLSSMSVFPVTAESVMPP
                     RDSPYLEYKLYLFEPRTVEVEAILAPTLNFVPGRGLRYAISFDDEAPQIIDALAQHAV
                     KDWETSVKDSVRKAKSTHSLAKSGYHTLKFWMVNPGVVLEKLVVDLGGVKPSYLGPPE
                     SYHRGRSQLAPN"
     gene            complement(7596..>7966)
                     /locus_tag="VGR03_03540"
     CDS             complement(7596..>7966)
                     /locus_tag="VGR03_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993384.1"
                     /translation="GFAPYWFDGNFKTYGDRVNNLSVDWHMLIALMAPRPVYIATAEQ
                     DRWGDPRGSFLAAKAAEPVYKLFGRAGLGADEMPPVETPVGNFIGFHNRKGSHGLNEY
                     DWEQFLNFADRQFGKEKVLK"
BASE COUNT         1714 a         2430 c         2146 g         1676 t
ORIGIN      
        1 ggccctcaga gcatcggcct gcaccaattc gccgccgatg cccaatgccg cagcgcctgc
       61 ttcgataaat tcagccgcgg tattcaggtt gacgccaccg gtcggaacaa gcggaacttg
      121 cggaagcggg cctttcagtg ccttgatgta tttcgcgccc cccaccatgc cgcaagggaa
      181 aactttgacg aaatccgagc cggcttccca agcggttatg acttccgtgg gcgtcagtgc
      241 cccggccatc atcagcttcc cttcccgcac cgcaagctcg acgacctgaa ggttgagccc
      301 cggactgacc agaaattgtg caccagcatc gaggcaacgg cgcgccgctt caagattgag
      361 gacggtaccc gctccaacga ggacgtcaga agctccgccc ttggtaagct cgcggatgac
      421 gtcgacagcg ccgggcacag tcatggtgat ttccacgatg ggtatgcccc cttcacaaac
      481 ggcgtcagtg gcaattcgcg cctcgcgcgg cgatgccgcg cgaacgaccg ggacgattcc
      541 aatctcaatg atccgatcgc gaatcttttg cttatccata ggcgatcacc tttcctccgg
      601 cgcttcctta acgcgcgata cgcgccccga ctcctttcat cacttgcagg acttcctgga
      661 gggttgccat cgaggtgtct cccggcgtgg tcattgccag ggcgccgtgg gcggcgccgc
      721 attccaccga ccactgcggg tcttgtcccg ctaggaaccc gtaaatcaag ccggaagcga
      781 acgaatcgcc tccgccgacg cgatcgaaaa tttcaatatt ttcgcggttt ctcgcctggc
      841 agaacttgcc atcgcaataa caaatcgcgc cccaatcatt caaagtcgcc gtcttcgctt
      901 tcctgagcgt ggtggcaaca accttgaacg gatagtcctt cacggccttc gcaatcattt
      961 gcttgaatcc ttcgacctcg aacgtcgaat gatgctcatc cattcccgga acctcgtagc
     1021 ccagcgccgc cgaaaagtcc tcctcattac cgagcatgac gtcgacgtgt ggcgcgatgc
     1081 gcctgttgac ctcctgcgcc tgttttttcc cgccatgaga tttccagagg gaggcacggt
     1141 aattcaagtc gtaggaaacg actgtcccgt ggcgcctcgc cgcctgcatg gcttccagcg
     1201 tgacttcggg agtcgtctga gaaagcgcgc agaaaattcc gccggtgtga aaccagcgcg
     1261 cgccctgttt tccaaatatc tgatcccaat cgatttcgcc aggcttaagt tgcgacactg
     1321 ccgtatgccc gcgatcgtaa cttcccagcg ccgcgcgtac gccgaagccg cgctccgtaa
     1381 aattgaggcc gttgcgcgcc gcgcgcccca cgccgtcatg ctccacccag cgaatcagcg
     1441 actggtccac gccgccctga tagatgaggt cctgaagaag ccgccccacc ggatcatcgg
     1501 cgaacgcggt gacaaccgcg gtgtccaggc cgaaacagcg cttcagcccg cgtgcgacgt
     1561 tgtattcccc gccgccctcg cacacttcaa aagatcgcgc cgcggcgatg cgccgctcgc
     1621 ccgggtcaag cctcaacatg acctcaccca aactgatcag gtcccatcgg cagtccttct
     1681 tcggcttgat cgtcaacccc atgcgctcac ctcgaccgcc cattcaaaat gaacattgtt
     1741 acccgctaca ttctcacgaa ttactcagaa tgattccaga ttcgcgtttg ggcctctcaa
     1801 ttaatcttgg ctggctccag cctgggcgcc aaaacatgga tggcggccag cgccaccaaa
     1861 tacacggagc cggcaatgaa aaacggaacc atgtaactgc cggtccactg taaaacatgg
     1921 cccactattt tggcgatgag catcccgccg accgcgcccg ccattccacc gatcccgacg
     1981 acggaggcca ccgctcgcga cggaaacata tcggaagtca gcgtgtaaag attcgcggag
     2041 aagccttgat ggcccgcggc agcaagacca atcagcagaa ccgccggcca caatcccgac
     2101 acgtgataag cagacacgat gggaacgacg gtaatcgcgc acagcaacat ggcgatcttg
     2161 cgtccagcgt tcacggaaac tccccgccgg atcaggaagg aggacaacca tcctccggct
     2221 acgctgccaa catcggagat gagataaatc actaccaccg gaagaccaat ttgagccagg
     2281 tgtaagccgt acctgcgttc cagaaaatcg ggcacccaaa aaagatagaa ccaccagatc
     2341 gggtcgatca agaattttcc gcaggcaaaa gcccacgtct gccggtacgg caggaggctc
     2401 aaccatttga tcttctgtgt aggctgcggc atatcgcccc ggatgtactc gagctcagcc
     2461 ttggaacagc gcggatggtc ttctggtctt tgataaacca agagccacaa tgccagccac
     2521 gcgaagccga gcgagccgac aatcacaaag gcccagcgcc agccaaagtg ggcggtgatc
     2581 cacggcacgg acaggccggc tagaatcacc cccatattcg tccccgcgtt gaaaatccct
     2641 gtagccagcg cccgctcctt cgcgggaaac cattccgcaa cggcctttat gctggctgga
     2701 aacactcccg attccccaaa gccaagcgcc acccgcgcca tcaggaagct ggagaacgag
     2761 cttcccacag cgtgagccat ggaagcgagg ctccaaaaga ccatgatgat ggcataaccc
     2821 aaacgcgtac cgaaacggtc gataaacctg cctacagcga gcatgccgac cgcgtaagcc
     2881 gcctgaaagg caaccaccag gtcactgtaa ttgatctcgt tccagccgag gtcatgctgc
     2941 agcgtcgtct tcaacgccga gagaacccag cgatccatgt agttcttcgt cgtgccgagc
     3001 agcaggaggc cgcagatcac ccagcggaaa tagccagggc gagagggagc agttggcaga
     3061 gttcgttcgc tcgccatcgc agcattcgtt tgcgtctgcg acgctgagac gccggatggg
     3121 gaagttgggc gcacacggtc catggtatga aggaaaagct actcgttctt gcgaccggct
     3181 tccagtccgg aagattcctc attgaatccg ggccgtacac tagaatacaa attccgcact
     3241 gaaaagttcc cttctaccaa ctgtgcatgg tgccgtcgag tttccggttg atcgggagat
     3301 acgcccggcg atatggatat ttggccgcca gctcttcatc gatatcgacg cccaggcccg
     3361 gggcatcgcc agggtgcatc attccgccgg aaaaggtata ggaatgggga aagacgcggt
     3421 cagtctcatc ggcatggcgc atatattcct gaatgccaaa attgtgaacg ctcaaatcga
     3481 aatgcagcgc ggccgccatg cacacgggac tcagatcggt tgcgccgtgc gatcccgtgc
     3541 ggacatggta gtgctccgcg aggctcgcga tcttccgtag atgcgagatt cctcccgcat
     3601 gcacaacggt ggtccgaatg taatcgatca gttgctcctg gataagctgc tggcaatcat
     3661 agatggagtt gaagacttcg cccacggcca atggcgtggt ggtgtgctgg cggatgatac
     3721 gaaagctttc ttgcagctcc cccggaactg gatcttcgag ccagaacaga tgatagggct
     3781 cgaggctttt gccggcgcgc cccgcttcga tcggcgtgag gcgatggtgc gcgtcgtgca
     3841 gcaaatggag atccgctccg aattctttgc gcagccgttc aaatagccgc ggcacgaagt
     3901 ttaggtacaa ctctgaactc caggcatttt cgaccggggc atctttttcc gctggctcat
     3961 aatgcatttt gccgcggcca acgccgtatg tgctctgcaa gccaggtatg ccgctctgcg
     4021 cgcgaatcgc tctatatcca agtttgagat agtcggcgac ggcttgaact gtttcttcga
     4081 tgtcctgccc gctggcgtgg ccatagacta ggacgccgtc gcgggaagct cctcccaata
     4141 attgatacac gggcacattc aaagccttgc ccttgatgtc ccaaagcgcg gtatcaaccg
     4201 cggcaatcgc actcatcgtt accggaccgc gccgccagta cgcgcccttg taaaggtact
     4261 gccaggtatc ctcgatccgc cgtgcatcgc gccccatcaa gagtggaatg aggtggtcgg
     4321 agagatagct tgcgacagct agctcacggc cgttcagggt ggcatcgccg aggccgtaaa
     4381 tcccgtcctc tgtcgtaatc ttcagcgtta caaagttgcg tcccgggctg caaacaatca
     4441 ctttggcatc ggtgattttc atcgttgata tggatcctct gtatcgcggc ccgcgaaacc
     4501 gtggaagaag ataaactcat accacatcca cggcctactc ggctcatgaa ctttgctcgc
     4561 ttactctaca accgttgtcg tcaacgggaa agccaaccgc caccagcaca tgcaccctct
     4621 gcggttcctc gagcagcgaa gttcttagtt cggagcaagt tgggaacggc cacggtgata
     4681 gctttcgggc ggaccgagat aactcggttt cacaccgccg agatccacca caagcttctc
     4741 cagcaccacg cccggattca ccatccagaa cttcaaagtg tgataacccg attttgccag
     4801 tgagtgtgtg gacttcgctt tgcgcacgct gtctttcacg gacgtttccc agtctttcac
     4861 cgcgtgctgc gctagcgcat caatgatctg aggtgcttcg tcatcgaatg agatggcgta
     4921 tcgcaggcca cggcccggca cgaaattcaa cgtgggggcg aggatggcct ccacttccac
     4981 agtacgcggc tcgaaaagat acagtttgta ttcgaggtac ggagaatccc gcggcggcat
     5041 cacgctttcc gccgttacag ggaacacgga catggacgag agagtccgtc cgaggtcgtc
     5101 aatcctttcc cagcgcgccg acgttccatc gactttcttg ccgtagtgta ccgcttccat
     5161 cgaaacgtac ccatcgctct ccacaaatcc gtccagcgat tctcgcgtca ggtcttgcgg
     5221 acgagaagcg tacaccttca cgacgacctc ttgcgcgctg ccccccgcga ttttcacgga
     5281 gccgctcgta gaacccacgg gggccttgtt ccaatccacg ctgacccaca cgcgccgttc
     5341 cttctcaatc gtcccctgcg attcgctcag cacgatccac ggcacgcttg aagtggcgga
     5401 aaatacaaat ggctcccggc ctcgattgaa tatgtcgatg tagcggcgct gctggttgaa
     5461 gaggtcaaac ggtgggagag tcggttcgcc gggagagccc ggccaagcaa gcgtcgatcc
     5521 ttcaacagcc acaccgagtt gcgccggggc aggaatctcg atctcggtga ccttgggcat
     5581 gacgttcacc ggcggctggt tccagaaggt atagccgatg tgcgtttgat ccatcatatg
     5641 gtcccacttg ccgtgcgcta gcgtgtggtt gtaataggcg gatagatccg cgtcggcctg
     5701 aaacaaggcg cgcgcctccg cagccatatc gttcgcgctc gcacggcctt ggctggcgta
     5761 gagattgttt ctgcccgcag tgatgtacag ctccgtcacg atcgcggacg ctttcgtggg
     5821 gtagagaaca agctcgaaga aagcatcgcg ctggttctcc ggcaacttgc cgtagatctc
     5881 ttccgctttc gccgccgcgg cctggaaatc agcgaaaacg gtgtccgcct cccggtagtc
     5941 caccaggctg aacgtgccgg gctccagcaa ctcgggtttg cgcctgccgt tgtatttcgt
     6001 gtacttcgaa agtatgtccg caatttccgg cgcgaactcc cgtccaaatt gccgctccgc
     6061 ccagagccgt gtgaattcag agattttctc tttgggccag ttttccggct tccacgccat
     6121 gttcagaaaa aaatcaatgg gcaattccat tggcttcagg tcacccacgt tgacgatcca
     6181 gattcgatcg gcgccgtagt gatacgcgag attcatctgc tcccaaacct tggcaatcgg
     6241 aatggtgttc aaccacttgt aggaccgcgg atcgccgacg taatcgaagt gatagtagac
     6301 tccagcgccg ccgctgcgtt ttcgttcctc ctgagtcggc aacctgcgga tatttcccca
     6361 gttgtcatcg caccacagca gcgtcacgtc gtcgggaacg cgcatgccct tctcgtagta
     6421 ttcctgcact tccttataga gggcccagtc ctgcggcact ttggataaat cgggatttac
     6481 gcggtccgcg atcattttgc gctgatccgc cacgatcttt tccagtaagg cgatgttagc
     6541 gctctcggac atggggaggt ctccgtctcc gcgcatggcg agcgtgatga tgctctcgta
     6601 ctttttgttg cgttcgacgc cttgatccca gaattccttt aggacttcgg catttttcga
     6661 ataatcccac ggaccttttc catgccgctt ccactcctgc tgagcgcgca gcatcggttc
     6721 gtgatgagat gtgcccatca caatgccgta ttcgtcggca agctggggat tcagcggatc
     6781 gtcctcgtta aaggcgttgt tccacatggc cggccagaga tagttggcct tcaatcgcaa
     6841 taggagttca aaaaccttgg tgtaaaactc atgattgtaa ttcccgtatt ttcctttcac
     6901 ccatccactg agagagggcg cttcatcgtt caggaaaatt ccgcggtact tgactgcagg
     6961 cggtccctgc acatggatcc ccggctttac aaagagttca tcgtgatgct ggacgggaac
     7021 gtcggcccac cagtaccacg gcgaaacccc aatctggccg gaaagatcat agactccgta
     7081 gattgttcct cgtttatcgc ttcctgcgat aatcagcgcg ctggccacgc ctggcagcgg
     7141 cttcggcacg acttgaatga gaaacgattc ccattttcgg gcgatggatg tcacatcgat
     7201 tttgcgatcg cggatcagtt gatcgatgat tttgctcttt ccaaccgtgc ccacgatgat
     7261 catattcggt ccaaggttct ttccgtcgtg cgcgatcgcc gtggtgcagt tcgaaacgcg
     7321 ctcgatgtcc gcctgcaaat cgttgacggc gcgaacaaca cctgggaagt catccgcttc
     7381 aacgaataga gttgcagcgg cgtttcgcgt gacgatactg aaatcaccgg cgtgataggc
     7441 cgggtccaca tagcgatcct cgccgatcgt gagagcgtga agaagaggag acgcgaggag
     7501 ataaaagatc aggaacaggc cagccgtacc taccgagcgc gtgcagcgaa tggcccaatc
     7561 ttccatgctg gggtgtcgtc gaagcggatc gcggatcatt tcaaaacttt ctctttgcca
     7621 aattgacggt cggcgaagtt caggaactgt tcccagtcat attcgttgag gccgtgcgag
     7681 cccttgcggt tgtgaaagcc aatgaagttg cccacgggcg tctccacggg cggcatttcg
     7741 tccgcgccta ggccggccct gccgaaaagc ttgtagacgg gctccgcagc tttggcagcc
     7801 agaaatgagc ctcgtggatc gccccagcga tcttgctccg ccgtggcaat gtatacaggg
     7861 cgtggcgcca tcaacgcaat aagcatgtgc cagtccacgg aaaggttatt cacccggtcg
     7921 ccgtaggtct tgaaatttcc atcgaaccag taaggagcaa agcccg
//
LOCUS       DASYVO010000107         4090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_72385, whole genome shotgun sequence.
ACCESSION   DASYVO010000107 DASYVO010000000
VERSION     DASYVO010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72385"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VGR03_03545"
     CDS             complement(<1..675)
                     /locus_tag="VGR03_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV1993385.1"
                     /translation="MNKRNLLTIGGAAALGLALIALPASSARPQKTNDSTIARLQQRI
                     DELEAKLQAQLDSRQDQKIELAGADDLEDSAQTVIVENQDPTRIEVLPEIETDDLNIV
                     IGDDGSSWLGVETHEVTADKAKELKLSAERGVVLGKIVPDSPAAKAGLKENDVLTEIN
                     GQRIEGAAQFRRMIHEIPAGRSIQLTVWRDGRTQTISATLGKSEERRHAMKMLAPTPG
                     AFAFRMP"
     gene            complement(723..1487)
                     /locus_tag="VGR03_03550"
     CDS             complement(723..1487)
                     /locus_tag="VGR03_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEV1993386.1"
                     /translation="MLRPALRATALWVLLLAVIWAVPSFAISKEFNQSYPLQPGGSLE
                     LQNVNGTVDVQGWDRNEVEVHAVKTAKHKESDLERVSIEVDAKPGAISIATRYPQNEG
                     VEVAVEYIIHVPHGARVEHIGTVNGTLRIAGVETVEDLHTVNGNIEVFEAGGSVHAHT
                     TNGNVHLELAHLPDKTGATAETTNGSLVLAVPSDMQADVEARCLNGNFSSELPITMES
                     TQRPREMHGKLGHGGAPIHLRTVNGGIRVVILRSTV"
     gene            complement(1571..3400)
                     /locus_tag="VGR03_03555"
     CDS             complement(1571..3400)
                     /locus_tag="VGR03_03555"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEV1993387.1"
                     /translation="MNIRFHHRLFSIARRVALFHVLAFLFSVARLAPPVFAAEAKEPA
                     ALERTQPADPGSQHASVVRRGVVGTKDGLTLRLTTDLGSVNIVQLEAGAAPIVRYTVR
                     IETDARGTAAQQLLDTYSLKAKSIPTGVEITGVLPPQAARSADAQFWVQFEVAVPRGY
                     SIEVTTEAGDITTADIGGTASLHTQGGNIKTGRIGSSGLRDAAWGRSAAKLETEGGHI
                     QVLDVAGDLTAHTGGGHINVGNIAGDASLRTGGGHIRAGQIGGRAELETVGGNITVAH
                     AGNFVSVRTGGGQIDFGEVRGSVHAQTGGGGIRVMYVSGPMELESSSGSICLTRVAGA
                     LQAATGGGTITAWINPDATSESGNVRLAGASQLSSGNGDIVVFLPRNLAANIDATVAN
                     GGERRIEADPALHLTMQASTNGSGPVHAMAVLNGGGAPLRLRTTGGKIRLQFLDSEIA
                     LHESLIREQVDRLNKRLKENGFDPVSFTPAVGPGIPTLPEMPTPSEAKTDWLESWLTS
                     LEITFRGGLTENPDDFQKRLVNSPKPSFPALAQRAGLQGLVKLQVRVKKDGSVEVQKL
                     LEGEPALADAAIAAVKQWRAKPAAINGKQVEVISTVTFNFQLH"
     gene            complement(3432..>4090)
                     /locus_tag="VGR03_03560"
     CDS             complement(3432..>4090)
                     /locus_tag="VGR03_03560"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEV1993388.1"
                     /translation="MNVMAKPPLTDDQLSKMLVGGINFSPSSGSGSPDVQLQLSAEQP
                     LVLTGNVQDSDMRRVLTYVVENGERFDAGVRLDCLEALKVAALDQQVRHALIAAARKD
                     LNPAVRMKALESLRDAASDDDVRQALLDALEHDANPGVRVEAVNVLVGSLQHGDSDEM
                     APEADNATQSVRPIRPEEDESVERVVRALEQLQRRDPSRYVRLRSAAALRQIGPREVQ
                     "
BASE COUNT          783 a         1236 c         1231 g          840 t
ORIGIN      
        1 cggcatccgg aacgcgaatg ccccgggggt cggcgccagc atcttcatgg cgtggcggcg
       61 ctcttcggat ttgccgagag tcgcgctaat ggtttgcgtg cgcccatcgc gccacacggt
      121 gagctggatg ctgcggcccg caggaatttc atggatcatg cggcgaaatt gcgccgcgcc
      181 ctcgatgcgc tgtccgttga tttccgtcag tacgtcattt tccttgaggc cggccttggc
      241 cgcggggctg tctggaacga tctttcccaa aaccacgccg cgctcggcag aaagcttcag
      301 ctctttggct ttgtcggcgg tcacttcatg tgtttcgacg ccgagccaac tcgaaccatc
      361 gtcgccaatg acgatattca gatcatctgt ctcgatctcc ggcaggactt cgatccgcgt
      421 ggggtcttga ttctccacga tcacagtctg tgcggagtcc tcgagatcgt cagcgccagc
      481 gagttctatc ttttgatctt gccgactatc gagctgcgcc tgtaatttcg cctccagctc
      541 atcgatcctc tgctgcaagc gtgcgatcgt tgaatcgttg gttttttgag gtcttgcgga
      601 actggcgggc agagcgatca gcgcgagccc gagggctgca gccccaccaa tggtaagcaa
      661 atttcgtttg ttcattgtcg tcccatcctc ctcagcagtt agacgcagca aaaggaaaag
      721 cgtcagacgg tcgaccgcag aataaccacg cgaatcccgc cattcacggt gcgcaaatga
      781 atgggagcgc cgccgtggcc caatttgcca tgcatttccc ggggacgctg cgtgctctcc
      841 atcgtgatag ggagttcgga agagaaattt ccattcaagc agcgagcttc gacgtccgcc
      901 tgcatgtcgg acggcacggc gagaacgaga gagccattgg tggtttccgc ggtggcgccg
      961 gtcttatcgg gaagatgcgc cagctccagg tgcacattgc cgttggtggt gtgtgcatga
     1021 acgcttccgc cggcttcgaa gacttcgata ttaccgttta cggtgtgcag gtcttccaca
     1081 gtttcgacgc cggcaatgcg cagcgtgccg ttcaccgtgc cgatatgttc gacgcgcgcg
     1141 ccatgcggaa cgtggatgat gtattccacc gcgacttcca cgccctcatt ttgcggatag
     1201 cgagtggcga tagagatggc gcccggcttg gcgtcaacct cgatagaaac acgttcaaga
     1261 tcggactctt tgtgcttcgc ggtctttacg gcatggacct caacctcgtt gcggtcccac
     1321 ccctgcacgt ccacagttcc gttgacgttc tgcaactcca aggaaccgcc gggttgcaga
     1381 ggataggatt gattaaactc cttgctgata gcgaagctgg gaacggccca aataacggct
     1441 agaagcaata cccatagagc agtcgcgcgg agagctggac gaagcatgac gttctacccc
     1501 cttcgagtac gtgccaccaa cgagaaccga ggacccgaac aaaagtcgcg tgcaccgata
     1561 cggctgccgc tcagtgcaac tgaaaattaa atgtaacggt cgaaatcact tcgacctgct
     1621 tgccgttgat ggcagccggt tttgcccgcc actgttttac ggcggcgatg gcggcatcgg
     1681 cgagggccgg ctcgccctca agaagtttct ggacttcgac gctgccgtcc ttcttcaccc
     1741 gcacctggag cttcactagg ccttgtaagc cggcgcgttg tgcgagtgcc ggaaacgaag
     1801 gcttcggaga attgacgagc cgcttctgga aatcgtcagg attctcggtg aggcctcccc
     1861 tgaaggtgat ttccaaactg gtcagccagc tttccagcca gtccgttttg gcttcggatg
     1921 gcgtcggcat ttccggcaat gtcggaattc ctggccctac tgctggtgtg aaggagactg
     1981 gatcaaatcc attttccttg agtctcttat tgaggcggtc cacctgttcg cggatgagcg
     2041 attcgtgaag agcaatctcc gaatccagga attggagacg aattttcccg ccggtggtgc
     2101 gcaatctaag cggggcgccg cccccattca aaacagccat ggcatgcacg ggaccggaac
     2161 cattggtaga cgcctgcatc gtcaagtgca gagcggggtc tgcttcgatg cggcgctcgc
     2221 ccccgtttgc cacggtagcg tcgatgttcg cggcgagatt ccgcgggaga aacacgacga
     2281 tgtcgccgtt gccggaggaa agctgcgaag cgccagcaag cctcacgttc cccgactccg
     2341 aagtagcgtc cggattaatc cacgcggtga tcgttccgcc tccagtagct gcctgcaggg
     2401 cgccggcgac gcgagtcagg cagatgctgc cactgctcga ctccaattcc atcggaccgg
     2461 aaacgtacat gacgcgaatc ccgccgccgc ccgtctgggc atgcaccgaa ccgcgcactt
     2521 cgccgaaatc gatctgcccg ccaccggtac gtacgctgac gaaattccca gcatgagcga
     2581 cggtgatgtt gccgccaacg gtttccagtt cggcgcgccc tccgatttgg ccggcgcgga
     2641 tatggccgcc cccggttcgc agcgaagcgt ctcccgcaat attgcccacg ttgatgtgtc
     2701 cgcctccggt gtgggccgtg aggtcacccg cgacatcgag cacctggatg tgcccgcctt
     2761 ccgtctccag tttggcggcg gagcgccccc acgcagcatc ccgaagtccg gaggaaccga
     2821 tgcgtcccgt cttaatgttt ccgccctggg tgtgaaggga agcggtgcca ccgatgtccg
     2881 ccgtcgtgat gtcgccggcc tcggtggtga cttcgatgct gtagccgcgg ggcacggcga
     2941 cttcaaactg cacccagaac tgcgcatccg cggagcgcgc ggcttgcggc ggaagcactc
     3001 cggtgatttc cactcccgtg ggaatcgact ttgccttgag agagtaagta tcgaggagtt
     3061 gctgagcggc ggtgccgcga gcatcggttt cgatgcgcac ggtgtaccgc acgatgggcg
     3121 cagcgccggc ctcgagttgg acgatgttga ctgatccgag atcggtcgtc agccgtagcg
     3181 tcagaccatc cttggtaccc accacgccac ggcgaacaac ggaggcgtgc tgcgaacccg
     3241 ggtcagcggg ttgggtgcgt tcgagggctg ctggttcttt cgcttcggcc gcaaaaaccg
     3301 gcggtgcgag ccgtgctaca gaaaaaagga acgccaacac gtggaatagc gcgacacggc
     3361 gcgcgataga aaaaagcctg tgatggaaac gtatgttcat cgtcgaacga aatttcttac
     3421 cgtctgaccg gttactgcac ttcgcgcgga ccaatctgcc gcaaggcagc ggcgctgcgc
     3481 aaccgcacgt agcggctggg atcccggcgt tgcaactgtt ccagcgcgcg aaccacgcgc
     3541 tccacggact cgtcctcttc cggacggata ggcctcaccg attgggtggc gttgtccgct
     3601 tcgggcgcca tctcgtccga atcgccgtgc tgcaaagatc caacaagcac attgacggct
     3661 tccacgcgca cgcccggatt ggcgtcatgt tccagcgcat cgagcagagc ctgccgtacg
     3721 tcatcatccg acgccgcatc gcggagcgac tccagggcct tcatgcgcac ggcgggattc
     3781 agatccttgc gagccgcggc gatcagagca tggcggactt gctgatccag cgcagccacc
     3841 ttcagtgctt cgaggcaatc gaggcggaca ccagcgtcga agcgctcgcc attttcaacc
     3901 acgtaggtga gtacgcggcg catatcgctg tcttgaacgt tgccggtgag caccagcggc
     3961 tgctcggcgc tgagctgaag ttgcacgtcg ggagagccag aacccgaaga aggcgaaaag
     4021 ttgatgcccc cgacaagcat cttcgaaagc tggtcgtccg taaggggggg cttggccatg
     4081 acgttcatgg
//
LOCUS       DASYVO010000108         7633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_171819, whole genome shotgun sequence.
ACCESSION   DASYVO010000108 DASYVO010000000
VERSION     DASYVO010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171819"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1774)
                     /locus_tag="VGR03_03565"
     CDS             complement(<1..1774)
                     /locus_tag="VGR03_03565"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1993389.1"
                     /translation="MAADVSKHLDRAKRFLEKNRVEDAIEAYLAVLDEMPQHQEATQA
                     LGDLYARMDQPDRAAVYYGLLFDLLVEPKDETKALAIYNRFLRTGHGQQPPERIARYA
                     FLQQKQNHPDEAIEQYTKAAELFAAASRDEDALFCWERGAQLDPENLSRQLRLAEAAE
                     RIGKTALAARAFLRAGQIATASGAAADALHLLGRAYKLAPQERSVGLLYAQANLQAGN
                     AVRAAALLESFAASESDPAFLATFSDALMRSGNLERARELLDRLLREKNEGMTQLFEL
                     ADRYVAAGQDQNAVEILQTLKRRMFADKRQNDFATLMDGVGAKYPHSQAILEFWASVH
                     NELNRESQYFEVLIHLFDVYLTSGNVAKAAESLERLVDIDAYDYRNQERLELLRGKVD
                     ETHLKRIASRLMKSSTPTPGHTSQTPAAQAPVVPSPGSEEGRQLQALEDLIVQTEIFL
                     QYSLQNKALERLQRIAAMFPGEEERSARLSNLYQMANWWPPGTAKPKAESAAAPPVVS
                     AAPLAPAAPVAEAPSPITTKTGVYSAETLRDLSKISEVNQKIFRQQTPRAMLNTAVNE
                     VGTYLRVTRALAVVGTPGRPPEMAA"
     gene            1989..2840
                     /locus_tag="VGR03_03570"
     CDS             1989..2840
                     /locus_tag="VGR03_03570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1993390.1"
                     /translation="MLQFPAIMTTTDSTLHYALGSTDAEHERLIRQAARLAPFTERFF
                     RDAGIGPGQRVLDIGSGAGDVAMLAANLVGPSGEVVGIERDPHSIARARSRVAEAGLH
                     NVIFTQSDVSQIPSSKPFDAVVGRFILMFLPDPLAILRSLTQLVRPGGVVAFHELSWA
                     PYLLYAARLPLWSAGASLVHETFRRSGANTEMGFALFQIFQQAGLPAPTMSLEMPLGH
                     DPEFTRWVPDVLGSLLPLVRQFNLPLEPLGDLDTLPERLQAEVAKSNSVVTWMALVGA
                     WSRRPIT"
     gene            complement(3134..3625)
                     /locus_tag="VGR03_03575"
     CDS             complement(3134..3625)
                     /locus_tag="VGR03_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1993391.1"
                     /translation="MKRYLFLTLAVAGLFAGAGLLFTSAAHDHSHEKNAFTPDTIPWG
                     PAPPVVRPGAQFAVLEGDPTASSGDFTIRLKMPDGFRIAPHWHPQRENVTIISGTFRV
                     GMGDDFDANTMKAFTAGSFAFLDPDMHHYAMACGETVVQVHGQSPLQFNYVNPDDDPG
                     RNR"
     gene            3774..5582
                     /locus_tag="VGR03_03580"
     CDS             3774..5582
                     /locus_tag="VGR03_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEV1993392.1"
                     /translation="MRRTRPAVGEIELDLGRYELRRLGRRVKLEKKPMELLIFLVGRR
                     EQLVSRKEIVAKLWRSDLFVDTQLNINNIVRKIRTALGDDSTKPRFLETVVGKGYRFI
                     GPVRVIDARYPQSDLGSGAMRAVSLETAGGRSERSSLAVLPLILLGKATDEHGLCLGF
                     ADALVTRLGNLPGVDVLPISAVLNAPAEATASETASRLGVRFVVHGAIQISKGQWRLS
                     MEMFDTHMQSACFTRKRDLDVNRLSDLEDEISKQIAAALNRPLGPGMVQRRPRYSKDP
                     LAYAEFIRGYRLSSSGDPALLDEAAQHLIDAVTRDPAFSLAHAALSLVCATRHFELDP
                     ASAWLDKAEFHCRRALELDPDLPEGHVAGAFLLWGPSKNFQHLEAIAELKRALALQNN
                     QPHAYNRLGTILAHIGLLDHAREMYERGRPFHPRKAVSHSIVQVYVWNREYDLAREEI
                     QAWRGENPGNKYPVYFAPQPAMMTGDWKEAKILLDEAVQLLPEDPLIVSLQGVFYALT
                     GKDGKALDCLTKACASPKSFGHAHHSYYQIACILAVLGQREAAFEWLERSVSCGFACW
                     PFFLKDPWLKNLRELPGFEVLVSSLQAKYPDHLGLL"
     gene            complement(5822..7141)
                     /locus_tag="VGR03_03585"
     CDS             complement(5822..7141)
                     /locus_tag="VGR03_03585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1993393.1"
                     /translation="MVANETDTALLPAEARTIHKVRMRIIPFVFVLYIVSFLDRINIG
                     FAALTMNKELVITSQQFGLVAGIFFFGYFLFEIPSNLLLHKIGARIWIARILITWGFL
                     AMLSGLVHSVHQLYAVRFLLGLAEAGYFPGIVLYLTYWFRQRDQAQMIALFLAGIPVT
                     SILGAPLSGFILDHVHWLSIGSWRWLLILEGVPAVVCGVLTYFLLPNRPAEAKFLNKE
                     EKDWIAAELAREEREKLASHKISAVQALMNRRVWHLGLIGFTLNTGMYSMNFWMPQLV
                     KSLSSGISNSLIGLLVMIPHLVGLPVMVMVSRSSDRKQERRYHAAIPAIAAGIALASL
                     GATHSIFPTIVLLSFAALGIYSVYGPFYCLPGDFLTGFAAASGIALVSSVANLGGFAG
                     PYAIGWISQKTGSLYGGLAVAGVSLFASATLMLLLPRRLRGNPVENR"
     gene            7496..>7633
                     /locus_tag="VGR03_03590"
     CDS             7496..>7633
                     /locus_tag="VGR03_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV1993394.1"
                     /translation="MDPKFIRNFCIIAHIDHGKSTLADRLLEMTGALTQREMHEQVLD
                     SM"
BASE COUNT         1575 a         2261 c         2161 g         1636 t
ORIGIN      
        1 ccgcggccat ttccggcggc cgccccggcg tgccgacgac ggccagcgcg cgcgtcacgc
       61 gcagataggt gccgacttcg tttacggccg tgttgagcat cgcccgcggc gtctgctggc
      121 ggaatatctt ttgattgact tcggaaatct tggaaagatc gcgcagcgtc tccgccgaat
      181 aaactcccgt cttcgtagtg atgggcgatg gcgcttcggc aaccggtgcg gcgggcgcga
      241 gcggcgccgc agacaccacg ggcggcgcag cagcagattc cgctttcggc ttggcggttc
      301 ccggtggcca ccaatttgcc atttggtaga gattggaaag ccgcgcgctc cgctcttctt
      361 ctccggggaa catcgcagcg attctttgca gacgctctag cgccttgttc tgcagcgaat
      421 actgcaaaaa aatctccgtc tgcacgatca gatcttccag cgcctgcaac tgccggccct
      481 cttcactgcc gggagaagga accacgggag cctgcgccgc aggagtttgt gaagtatgac
      541 ccggcgtcgg agtgctcgat ttcatcagcc ggctggcgat ccgtttcagg tgggtttcat
      601 ccactttgcc gcgcagcagt tccagccgct cttgattccg atagtcgtac gcatcgatgt
      661 ccaccagccg ctcgagtgac tctgccgcct tcgcgacatt tcccgaggtc aggtacacgt
      721 cgaaaagatg gatcagcact tcgaaatact gtgattcgcg attcaattcg ttgtggaccg
      781 aagcccagaa ttcaagaatg gcctgcgaat gcgggtactt ggctcccaca ccgtccatca
      841 gagtggcgaa atcattttgc cgtttgtcgg caaacatccg ccgcttcagc gtttggagga
      901 tttccacggc attctggtcc tgacccgcgg cgacgtaacg gtccgcgagt tcgaacagct
      961 gggtcatgcc ttcatttttt tctcgcagaa gccggtcgag caactcgcgc gcgcgctcga
     1021 ggtttcccga tcgcatgagc gcgtcgctaa aagtggccaa gaacgcgggg tcactttcgg
     1081 atgcggcaaa agattccagc aaggctgcgg cacggacggc attgccggcc tgcaggtttg
     1141 cctgcgcgta gagcagtccg acgctgcgtt cttgcggcgc gagtttgtac gcacggccaa
     1201 gaaggtggag cgcgtcggcc gccgcgccgc tcgccgtcgc gatctgcccg gcccgcagga
     1261 acgcccgtgc ggcaagcgcg gtcttgccga tgcgttcggc cgcttccgca agacgaagct
     1321 gccgcgaaag attttccgga tcgagctggg cgccccgctc ccagcagaag agcgcatctt
     1381 cgtcgcggct cgccgccgca aagagttccg cggccttcgt gtattgctcg atggcttcgt
     1441 caggatgatt ttgtttttgc tgcaggaagg cgtatcgagc gatgcgctcg ggcggctgct
     1501 ggccatgacc cgttcgcaga aaccggttgt agatggcgag agccttggtt tcgtccttgg
     1561 gctccacgag cagatcaaaa agaagaccgt aatacaccgc cgcgcggtcc ggctgatcca
     1621 tccgcgcgta gagatcgccg agcgcctgtg tggcttcctg atgttgcggc atctcgtcga
     1681 gcacggcgag ataggcctcg atcgcgtcct cgacgcggtt cttctcgagg aagcgcttgg
     1741 cgcgatccag atgcttgctt acgtctgcgg ccatccgttc aggttgcctt tgcggtgact
     1801 gaaaatcgtc aatcaggtgc ccaatagatg atggaacacg ggctgcacgt tgcttcgtac
     1861 gcgaactgcc cttgttaact atataccccc agcgggaagc ggcaagacac acgaaatgcg
     1921 ctgatgcggt atgcatcacc ggaagcagtg ccgctcgtaa tcagccgcac cgatcgcgga
     1981 ggcactcggt gctacaattc ccggccatca tgacgacgac agattccact ctgcattacg
     2041 ctctcggcag caccgacgcg gaacacgagc gactcattcg tcaggctgcg cggctcgctc
     2101 ccttcaccga acgattcttc cgtgacgcag gcattggccc aggccagcgc gtcttggaca
     2161 tcggttccgg cgccggtgac gttgccatgt tggcggccaa cctggttggt ccttcgggag
     2221 aagtggtagg catcgaacgc gacccgcact ccatcgctcg ggctagatct cgtgttgccg
     2281 aggccggcct gcacaacgtg atcttcaccc aatccgatgt ctctcaaatc cccagtagca
     2341 aacccttcga cgccgtcgtg ggccgtttca ttttgatgtt tcttcccgat ccccttgcta
     2401 tcttgcgctc tctgactcag ttggttcgtc ccggcggagt cgtcgctttc cacgagctat
     2461 cgtgggcgcc gtatcttctt tatgcggcgc gcttgccgct ctggtctgcg ggcgcttctc
     2521 tagtccacga gactttccgg cgttccggtg caaacacaga gatgggcttc gcccttttcc
     2581 aaatcttcca gcaagcgggg ctgcctgcgc caaccatgag tctggagatg ccgctcgggc
     2641 atgatccgga attcacgcgt tgggtccccg acgttcttgg cagtttgctc ccgctagttc
     2701 ggcaatttaa tcttcccctc gaaccgctcg gtgatctcga cactcttcca gaaaggctcc
     2761 aagccgaggt tgctaaatca aactcggtcg tcacttggat ggccctcgtc ggcgcctggt
     2821 cccgcaggcc gataacctga gcctccagct agtgggggtt tctcacgaag gtgctccctt
     2881 gggttaggcc caggaagcgg ttgccgccgc ttcctaggcc gtctaccgtg cgcttgactg
     2941 ctttttgttg ccggattaaa cgctaggctt gaggcttagt tgctcgtgga gtgaatccac
     3001 taaaagcacc tttgcggaaa cgaatggtcc cgcgcgcctc ctaacctcgt tcatacaaga
     3061 atctaggttc atgaatacgc ggacgagcgc ccatggaatc gcgttctcgc ctatcctgcg
     3121 ctcgatggtc ggactaccta ttcctgccag ggtcatcgtc aggattgacg tagttgaact
     3181 gaaggggaga ttggccgtgg acctgcacga cggtttctcc gcaagccatg gcgtagtggt
     3241 gcatgtcggg gtcgagaaag gcaaagctcc cggcagtgaa cgctttcatc gtgttggcgt
     3301 caaaatcatc ccccatcccg actctgaacg tcccagagat gatagtgacg ttttcccgtt
     3361 gagggtgcca atggggcgcg atgcggaacc cgtcgggcat cttcagacga atagtgaagt
     3421 cgccgctgga ggcggttggg tcgccttcga gcacagcgaa ctgcgcgccc gggcgcacaa
     3481 cgggcggtgc cggaccccag ggaatggtat ccggggtgaa cgcgtttttt tcgtgagaat
     3541 ggtcgtgcgc ggcactggtg aaaagcagcc ctgccccagc gaacaatccg gccactgcga
     3601 gtgtaaggaa caagtatcgt ttcatgattg agcctccgtc gttcgtatcg aacggcggga
     3661 atcttgactt gagcagagga ccatttcaat gaagagcgaa tacagtttgc atatctttcc
     3721 catatcgtcg caaattactg aatcctagga gataatgcgc ctgcgagagc cttatgcgac
     3781 gtacgagacc ggcagtgggc gagatcgaac tcgacctcgg gcgctacgag cttcgccgcc
     3841 tgggacggcg agtaaagctc gagaagaagc ccatggagct gctgattttt ctggtgggcc
     3901 ggcgagaaca actggtctcg cgcaaggaga tcgtcgcgaa attgtggcgc tcggatctct
     3961 ttgtcgatac ccagctcaat atcaacaaca tcgtgcgaaa aatccgtacg gcactgggtg
     4021 acgactccac taaaccgcgc tttctagaaa ccgtggtggg caaagggtac cgattcatcg
     4081 gaccggtccg tgtgattgat gcgcgctatc cgcaatccga tcttggatca ggcgccatgc
     4141 gcgcggtcag cttggagaca gcgggcgggc ggagcgagcg ctcttcgttg gccgtcttgc
     4201 cgctaatcct tctcgggaag gccacggacg agcatggcct atgcctgggg tttgccgatg
     4261 cattggtcac gcgtctcggg aatctaccgg gcgtggacgt tctgccgatt tcagcggtgc
     4321 tgaacgcgcc cgccgaagcg acagcttcgg agaccgcttc ccgtcttggc gtgcgctttg
     4381 ttgtccacgg cgccattcaa atatcaaaag gccaatggcg cctctcgatg gaaatgtttg
     4441 acacgcacat gcagagtgct tgcttcacaa ggaaacgcga tctcgacgtc aaccgcctat
     4501 ccgaccttga ggacgagatt tcgaagcaaa tcgccgcggc attgaaccgg ccgctgggtc
     4561 cagggatggt ccaacgccgc ccacgatata gcaaggaccc tctggcatat gcggaattca
     4621 tacggggcta ccggctcagt tcctctggcg atccagcgct gctcgacgaa gcggctcagc
     4681 acctgatcga tgcggtcact cgagatcccg ctttttccct ggcgcatgcc gccctttccc
     4741 ttgtgtgcgc gactcgccac tttgaacttg atccagcgag cgcgtggttg gataaagccg
     4801 agtttcattg ccgtcgcgcg cttgaactgg atccggattt gcccgaaggg cacgtcgccg
     4861 gcgcttttct cctatggggg ccgtcgaaga acttccagca ccttgaagcg attgccgaac
     4921 tcaagcgagc ccttgccctg cagaacaatc agccccacgc ctacaaccgt ctgggcacaa
     4981 tcctggctca catcgggctt ctcgatcacg ctcgagaaat gtatgagaga gggcggccat
     5041 ttcatcccag aaaagccgtt agtcacagta tcgtgcaggt ctacgtctgg aatcgagaat
     5101 acgacttggc gcgggaggag attcaagcgt ggcgcggcga gaatccaggc aacaagtatc
     5161 cagtctactt tgctccgcag ccggccatga tgaccgggga ttggaaagag gcaaaaatcc
     5221 tgctggacga agcggtccag ctcttgcccg aggatccgct catcgtcagt ttgcagggcg
     5281 tcttttatgc gttgacggga aaagacggaa aggcgctcga ctgcttgacc aaggcttgtg
     5341 cgagcccaaa atcctttgga catgcgcacc acagctacta tcaaatcgca tgcattcttg
     5401 ccgtgctcgg gcaacgcgaa gctgcgttcg agtggctgga gcgaagtgtt agctgcggat
     5461 tcgcatgctg gccttttttt ctgaaggacc cttggctcaa gaatctacgc gagcttccag
     5521 ggtttgaggt cttggtgagc tcgttgcaag ccaagtatcc tgatcacctg ggactgctct
     5581 agctccactt cctcacagtt tgcgcgtcgc gtattgaatt ttattagtag ggcgcagtac
     5641 cgctgcgccc ttactcgcca tggcctttga agtcgtcgcc cctcggcgat tgtcattctt
     5701 cgctcatccc ccaaggatgg gcgaagaatc tcagccgatg atccacgcaa accgtttttc
     5761 tagtctcgca attctcacaa gatctagccc gcccgcgggt gggctgtttc cacaaacttg
     5821 ctcaccgatt ttcaactggg ttgccgcgca gtcttcttgg aagcagcaac atgagtgtgg
     5881 ccgatgcgaa caacgaaacg cccgccacag ccaagccacc gtacagactc cccgttttct
     5941 ggctgatcca accaatcgcg tacggtcctg caaatccgcc aagattcgca accgaactca
     6001 ccaaggcaat ccctgaggcc gccgcaaatc ccgtgaggaa atcgcccggg agacaataaa
     6061 aagggccata aacgctgtag atcccgagcg ccgcgaacga tagaagcacg atcgttggga
     6121 aaatggaatg agtcgcgccc agtgacgcaa gagcgattcc agccgcgatg gctggaatcg
     6181 ccgcatgata tctcctctct tgcttgcgat cggaactccg cgacaccatc accatgacgg
     6241 gcaggcccac aagatgcgga atcatcacca ggagtccgat caagctgttg gagattccgc
     6301 tcgacagtga tttcaccagc tgcggcatcc aaaaattcat cgagtacata ccggtattca
     6361 aagtgaatcc gatcagtccc agatgccaaa cccgcctgtt catcaatgcc tgtactgccg
     6421 agatcttgtg gctcgccagt ttctcccgct cttcgcgtgc cagctcagcc gcaatccagt
     6481 ctttttcttc tttgttcaaa aatttggctt ccgccggccg gttgggcaac agaaagtacg
     6541 tgaggacacc gcaaactacc gccggaacgc cctccagaat caggagccac ctccaactgc
     6601 ctatgctcag ccagtgcacg tgatctagga tgaacccgga gagcggagcc cccaggatgc
     6661 tagtgaccgg gatgccggcc aggaacagcg caatcatttg tgcctgatcc cgctgacgaa
     6721 accaatacgt cagatacaag acgattccag gaaaatagcc cgcttccgcc agccccagta
     6781 aaaaacgcac cgcgtacagt tggtgcaccg aatgcaccag gccgctcagc atggccagaa
     6841 atccccaggt aatcaggatg cgcgcgatcc agatccgcgc ccctatcttg tgcaacagca
     6901 gattgctggg aatttcaaac aggaagtacc caaaaaagaa aatccccgcg accaatccaa
     6961 attgctggct ggtaatgacc agctccttgt tcatcgttaa cgccgcaaac ccgatgttga
     7021 tccggtcgag aaaggaaacg atgtaaagaa caaacacaaa cggaataatc cgcatccgca
     7081 ctttgtgaat cgttctggct tcggcaggca aaagagcggt gtctgtttcg ttggccacca
     7141 tcacctcagt gtgtgtccgc ctcgctcgat gggtcacctc ggccggcatc gctggccgaa
     7201 ggcgcgtgag gattgccgag actctatggc gggaaagtct gcccgtcaag taactccgga
     7261 tttccgcaag aagcaatcct cacttgccgg gttctgcttt cgtctcgcaa ccttgctttt
     7321 cgcatgagac gggcggatag gctgacgagg cctgcgaatt gtggcaaact gaatgattat
     7381 acggcacggt ttatgcgctg cgtgtgtcgc tcgttcgaag cagcaagccc gccgaaacaa
     7441 tcgggcaggc gattcgaatc ctgcccgcaa atgtgaaaat tggtaagcga gaaaaatgga
     7501 cccgaagttc attcgtaatt tctgcatcat cgcgcacatc gaccatggca aatccacgct
     7561 ggcggaccgt ctgctggaaa tgaccggtgc gctgacgcag cgcgaaatgc acgaacaagt
     7621 cctcgactcc atg
//
LOCUS       DASYVO010000109         6784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_201102, whole genome shotgun sequence.
ACCESSION   DASYVO010000109 DASYVO010000000
VERSION     DASYVO010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6784
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201102"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="VGR03_03595"
     CDS             <1..1309
                     /locus_tag="VGR03_03595"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1993395.1"
                     /translation="IEINTTDSVGNVTTAAEAMAKRLRDGGFAEGDIKVAGPNERKKN
                     LVARFRGAGKRKPILFIGHLDVVEALRTDWTTNPFEFIEKDGYFYGRGTEDMKEGDAI
                     LVANFIRLKKEGYLPDRDLILALTADEEGGSSNGVDWLLREHRDWVDAEYCINLDGGE
                     FEKDKDKRLLAGIQASEKVYVDFQFESLNSGGHSSVPSADNAIYHLSGALSRLQSYSF
                     SVKINEITRSYFERNAAMTAGQVSADMKAVAKQPPDFAAVQRLSAVPYYNSLLHTTCV
                     ATMLSGGHAPNALPQTARANVNCRIFPGEDPEEVHKTLERVANDPKVSVSIVPQLTPD
                     GKVVPVVPVPPSPLLPEVTQAMEKVLRIAWPGVPLVATMSTGATDGKYTRIAGIPTYG
                     ISCMFFDKNDNREHGKDERVGVQDFYDGVEFNYKLIRALSAPR"
     gene            complement(1407..3098)
                     /locus_tag="VGR03_03600"
     CDS             complement(1407..3098)
                     /locus_tag="VGR03_03600"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEV1993396.1"
                     /translation="MRKVATTIFVLILSGLGAIAATAAPRPADQARTMSDVIDRVITN
                     ENRANQQIKQYSPLVETYIQNLKPDKDLGYAPAGDKYFLGRADFSKGVSLVSLTDTNS
                     KGKKIFGSIGNFFSFAMQFLPDGFLQMIFIDTNGFDKQHYKFDYVRREFLGEVRCLVF
                     DVTPQDKAGKGRFLGRIWVEDQDYNIVRFNGAYGGGGHSSWYFHFDSWRTNVQPGVWM
                     PSFVYSEEKDLHYALAKKLDFKAQTRLWGYNLGHASQEQELSKILVETPVQDDSKTAN
                     DLSPIQAQRSWDRQAEDNVIDRLERIGLIAPRGEVDKVLETVVNNLEVTNNIDVEPDV
                     RCRVMMTSTLESYTVGHTIVMSRGLIDVLPDEASLATMVAHELSHVVLGHRLDSSYAF
                     FDQLLIDDKETFRHFGFARTPDEEKAANTKAVQLLNNSPYKNQLGNAGLFLTALGTRE
                     KEIPNLISPHLGDRVPIMSDLRSSTPVDKKQNPQMIAALPIGGRVKVDPWNDKLELIK
                     SKPVGSVAEREKMPFEVTPFMPYLMRYGAEASKPIAASAATPTNPRTGESAPGKP"
     gene            complement(3112..4275)
                     /locus_tag="VGR03_03605"
     CDS             complement(3112..4275)
                     /locus_tag="VGR03_03605"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEV1993397.1"
                     /translation="MIADPPRKTAMGVGFEATRGRSGDPQAWKKAIGTKPIAARLALL
                     PESKPRWDRIGLSALGQLAIFGFLLMIPLLFPTQMKTALSFRVVELMQPVTEIPIAPP
                     PPPPPKMKAKVVKPEPKPVVPEPVKLNPRQTHVFLAAKVAPPKIKTMEAKPVELNPVF
                     EQVKIDTPTNQPKRPKEDVKVGNLGSGSAAPATVVAPVNKVQTGGFGDPNGVAGKGDP
                     NKATNVNRLGSPALPGGPGYGNGTGGDKGIRGTVASTGFGNGTANPPPSAKRGTLQST
                     GFADQTVATEAPKKKASTGEGATTPVDILEKPRPEYTAEGRTLKIEGDVVIEMVFLAN
                     GSIQVNRVVSGLGHGLDETATRAAQQIKFKPARREGQAVDFPARVRIEFRLAY"
     gene            4676..5671
                     /locus_tag="VGR03_03610"
     CDS             4676..5671
                     /locus_tag="VGR03_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1993398.1"
                     /translation="MTEDAPKRGGQNPSVDLARPFDGQTSLLEWVYAQSQAGRWGVPR
                     ERFAAALERGAKKALTPGAATPQKLEEYLRALHLEDLALATACADGGEDAWEHFFATY
                     RAYLRAAAAAILRCSAGSAEACDLADSLFSELYGLAGGKGTERSLFRYFHGRSSLKTW
                     LRAVLAQRHIDSIRAGRRFEELGEDETGHGRPKTLLGPHVQPGDPHRERYVASFTRAL
                     HTVLERLEPQEKERLRLYYAEEKTLAEIGRLFGEHESSVSRHLDRVRRDLRQAVEDLL
                     RQGIGAANGFAVQPGLSEAEIALCFEYSAGDNPIDLEKLLPRSKSPGPAAGKRTP"
     gene            5723..>6784
                     /locus_tag="VGR03_03615"
     CDS             5723..>6784
                     /locus_tag="VGR03_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993399.1"
                     /translation="MAPDERDRSFDKALARHLRSAAPAGEAAGLPEIPASRSGACPDS
                     EMLAAYHERSLLPEQLNSLKEHIVGCANCQNVLAHLETTDEISLQAAQQDEVLAVKES
                     EPVTAARNLESFSAAAAPGRSQSAAGAALPKRSRRLLLLRGARWQWLAPAGAIAAGLL
                     VWIALHENQPSAPPSLKQVQVATNQAPPPPAPSVSKAVQGISPSPRADLTKPQSAAGG
                     YAGENGRGASGATKSDGKQEYLAQALPSKPLVDKEGRLRKDTARDSSVDSLRAENQRD
                     RDTKTVPGARQENAEVQLQAQAANVQSQNQSNANSPKVAGPAPLGQMEAKKMKAASPA
                     PAPPSAVGGVAGYTGGASMQ"
BASE COUNT         1335 a         2020 c         2024 g         1405 t
ORIGIN      
        1 cattgaaatc aacaccaccg attcggttgg caacgtcacc accgctgctg aagcgatggc
       61 caagcgtttg cgcgacggag gcttcgccga aggcgatatc aaggtagccg gcccgaacga
      121 acgtaagaaa aatctcgtcg cccgttttcg cggagccgga aaacgcaagc ccatcctttt
      181 catcgggcac ctggatgtcg tcgaagcgct ccgaaccgac tggaccacca atcccttcga
      241 attcatcgag aaggacggct acttctacgg ccgcggcacg gaggacatga aggaaggcga
      301 tgccatcctg gttgccaatt tcatccgctt gaaaaaggaa gggtatctgc cggaccgcga
      361 tctgatactt gcgctgaccg ccgatgagga aggcggctct tccaacggcg tggactggct
      421 actcagggaa catcgcgact gggtggatgc ggaatactgc atcaatctgg acggaggcga
      481 attcgaaaag gacaaagaca agcgcctgct cgcgggaatt caagccagcg aaaaagtcta
      541 tgtggatttt caatttgaat cgctgaattc cggcgggcat agctcggtcc ccagcgcgga
      601 caacgcgatc taccaccttt ccggtgcgct gtcccggctt caatcgtatt ctttctccgt
      661 aaagatcaac gaaatcacgc gcagttattt cgaacggaac gcggcgatga cggctggaca
      721 ggtttctgca gacatgaaag ccgtggccaa gcaaccgccg gattttgcag cagtgcaacg
      781 gctctcggca gtgccctatt acaattcgct gttgcacacc acttgcgtag ccaccatgct
      841 ttccggcggt cacgcgccaa acgcgctgcc gcaaaccgcg cgcgccaacg tcaattgccg
      901 gatttttcca ggggaagacc cggaggaagt tcacaaaacg ctcgagcgag tggcgaacga
      961 tccgaaggtc agcgtcagca tcgttccgca gcttactccg gacggaaagg tggtgcctgt
     1021 cgtgccggtg cctccgtcgc cgctactccc cgaagtgacg caagccatgg agaaagtctt
     1081 gaggatcgcc tggccaggcg ttcctctcgt tgcgaccatg tccaccgggg ccactgacgg
     1141 aaagtacacg cgcatcgccg gcattcctac ctatggcatc tcttgcatgt tttttgataa
     1201 gaacgacaat cgcgagcatg gaaaagacga acgcgtcggc gtgcaggact tctacgacgg
     1261 cgtggagttc aactacaagc tgatccgggc gctctccgca ccgcgctgag gaacacctgc
     1321 aagaaagaga taccgcgaag caataaaacg gcccggtgga ggtctcccca ccgggccgtt
     1381 gatttttcgt tctccagcct tggtgactac ggcttgcccg gcgcggattc gccggttctc
     1441 ggattggttg gtgtggccgc gctggccgcg atgggtttcg aggcctcggc gccgtaacgc
     1501 atcaggtaag gcatgaacgg ggtgacctcg aacggcattt tttcacgttc cgccactgag
     1561 cccaccggct tgctcttgat gagctccagc ttgtcgttcc aaggatccac tttcacacgt
     1621 ccgccaatcg gaagcgccgc gatcatctgc ggattctgtt tcttatcgac cggcgtcgag
     1681 gacctgagat cactcatgat tggaacgcga tcgcccaggt gcgggctgat caagttagga
     1741 atttccttct cgcgcgtgcc gagcgccgta aggaagaggc cggcgttacc gagctgattc
     1801 ttgtagggcg aattattcag gagctggacc gctttcgtgt tggcggcctt ctcctcatcg
     1861 ggagtccgcg caaatccgaa atggcggaag gtttccttgt cgtcgatcag caactgatcg
     1921 aagaaagcat agctggaatc gaggcgatgg ccgagaacga cgtggctgag ttcgtgcgcg
     1981 accatggtcg ccaggctggc ctcatccggc agcacgtcga tcaacccgcg gctcatcacg
     2041 atggtgtggc cgacggtgta ggactccagc gtggaagtca tcatcacccg gcagcggaca
     2101 tcgggttcca cgtcgatgtt gttggtgacc tcgaggttgt tgactaccgt ttcgaggact
     2161 ttgtcgactt cgccgcgcgg cgcgatcagc ccgatgcgct ccaggcgatc gatcacgtta
     2221 tcttccgcct ggcggtccca ggagcgttgc gcttggatgg gggaaaggtc gttcgccgtc
     2281 ttggagtcgt cctgcaccgg cgtctcaacc aggatcttgc tcaattcctg ttcctggctg
     2341 gcgtggccga ggttgtaacc ccacagccgc gtctgcgctt tgaagtccag tttcttggcc
     2401 agggcatagt gcaaatcttt ttcttcgctg tacacaaagg aaggcatcca cacgcctggc
     2461 tgcacattgg tgcgccagct gtcgaagtga aaataccagc tggagtgccc gccgccgccg
     2521 tacgcgccgt taaagcgaac aatgttgtaa tcctgatctt cgacccagat gcggccaagg
     2581 aaccggccct tgccggcctt atcctggggc gtgacgtcga acaccaggca gcgcacttcg
     2641 ccgaggaatt cccggcgcac gtaatcgaac ttgtagtgct gcttgtcgaa gccgttcgtg
     2701 tcgatgaaga tcatctgcag gaagccgtct ggcaggaatt gcatcgcgaa agaaaagaag
     2761 ttgccaatgg agccgaagat cttcttcccc ttgctgttgg tgtccgtcaa ggagaccagc
     2821 gagacgccct tcgagaagtc ggcgcggcca aggaaatact tgtcgccggc gggggcgtag
     2881 ccgaggtcct tatcgggctt caggttctgg atgtacgtct caaccagcgg cgaatactgc
     2941 ttgatctgct ggttggcgcg gttttcgttg gtgatcacgc ggtcgatgac gtcgctcatg
     3001 gtccgcgcct gatcggccgg cctgggggcc gccgtggcgg caatggcgcc cagcccgctc
     3061 aagatcagaa cgaaaatagt tgtcgctacc ttacgcattt tctctctccc cttaataagc
     3121 caaacggaat tcaattcgca cgcgcgccgg gaaatccaca gcctgacctt cgcgtcttgc
     3181 cggcttgaac ttgatctgct gcgctgcgcg agttgctgtt tcatcaagac catggcccag
     3241 accgcttacc acccggttca cctgaatgct gccgttcgcc agaaatacca tctcgataac
     3301 aacgtcacct tctattttca gagtacgtcc ttccgcggtg tactccggac gcggtttttc
     3361 caggatatca acaggcgtcg tggcgccttc ccccgtcgac gcttttttct tgggcgcctc
     3421 ggtcgcgacc gtttgatccg cgaaaccggt ggactgcagc gtgccgcgtt tggcgcttgg
     3481 cggaggattc gccgtgccgt taccgaagcc ggtgctcgcc accgtgccgc gaattccttt
     3541 atcgccgccg gtgccgttac cgtatcccgg tccacccggc aatgccggcg agccaagacg
     3601 gttgacgttc gtggctttgt tcggatcgcc cttaccagca actccgttgg gatcgccgaa
     3661 gccgccggtt tgcactttgt tcaccggagc cactacggtt gccggagccg cgctgcctga
     3721 tccaagattc ccgactttca catcttcttt cggccgcttc ggctgattcg tcggcgtatc
     3781 gattttcacc tgctcgaaaa ccggattcag ttcgacgggc ttcgcttcca tggtctttat
     3841 cttgggtggc gcgactttgg ccgcgagaaa aacgtgcgtc tgtctggggt tcagcttcac
     3901 cggttcgggc accaccggct ttggttccgg cttcacaact ttggccttca tcttaggcgg
     3961 cggtggaggc ggtggtgcta tggggatttc cgtaaccggc tgcatcaact ccacgacacg
     4021 gaaactcagc gccgttttca tctgcgttgg gaagagcagc ggaatcatga gcaggaaacc
     4081 gaagatcgca agttgtccga gggcgctcag gccaatgcgg tcccagcgtg gcttggattc
     4141 agggagcaag gcaaggcgtg ccgcaatcgg tttggtgccg atggctttct tccacgcttg
     4201 tggatcgcct gaacggccgc gggttgcctc gaaaccgacg cccatcgctg tcttacgtgg
     4261 tggatctgca atcatggtta tcatccgcgt gccgcctcgt taaattccgg ccacggaaca
     4321 tgggctgtct aggaattcct agggctccca agtccgccaa cagaatagaa agtgcgctct
     4381 agaagtacca gagtactgac gaggctgagg gagcccgaat aggactttcg tactagcgtc
     4441 gcgaagtcgc taatgctccc gcgagcgcac cgttgcgcac cctgttctaa cccggcgagg
     4501 cagtttcagt tgcgagtctg gggcgcaaca ccccctagga taccgcaatt ccgggcgggg
     4561 tagttcgagt tttgcgcacc actttccgtc tcctgggctt atgctctagg atgcaactgg
     4621 tggccccggc gttggtttgg gtctatcagg gggctttgcc cttctcgctt gacaaatgac
     4681 tgaagacgca ccaaaacgcg gcggtcagaa tccatccgtg gatctcgccc ggccgttcga
     4741 cggccaaacc tctctcctgg agtgggtcta cgcccagtcg caggcaggcc gctggggagt
     4801 tccgcgggaa cgatttgccg ccgcgctcga gcgcggcgcc aagaaggcct tgacacccgg
     4861 agcggcgaca ccgcaaaagc tcgaagaata tctacgggcg ctgcatctcg aagatttggc
     4921 tctggccacc gcttgcgccg atggaggcga ggacgcctgg gagcattttt tcgccaccta
     4981 ccgcgcttac ctgcgcgcgg cggcggccgc aattcttcgc tgcagcgcgg gttccgcgga
     5041 agcttgtgat cttgcggatt cccttttttc ggagttgtac ggtttggccg gtggcaaagg
     5101 cacggaacgt tcgctcttcc gatactttca cggacgcagc tcgctgaaga cctggctccg
     5161 cgctgttctt gcccagcggc acattgattc catccgggcg ggccggcgct tcgaggagct
     5221 tggcgaggac gaaactggcc atggacggcc gaagacgctg ctcggaccgc atgtgcagcc
     5281 cggcgatccg catcgcgagc gttacgtcgc gtcattcact cgcgccttgc acaccgtgct
     5341 cgaacgcctc gaaccacagg aaaaggagag gctccgcctc tattacgccg aggaaaaaac
     5401 acttgccgag atcgggcggc tgttcgggga gcatgaatcc agcgtttcgc gccatctgga
     5461 ccgcgtgcgt cgcgatttgc gccaggctgt ggaagacctt ctgcgacaag gtatcggggc
     5521 ggcgaacggt tttgcggttc agcctggact gagcgaagcg gagattgctc tctgtttcga
     5581 atacagcgcg ggagacaacc ccatcgattt ggaaaaattg ctgccgcgct caaaatcgcc
     5641 gggcccggca gccggaaagc ggacgccgtg accgcgcggt gcaagaaagc tgcggagttt
     5701 cgttctaagg atggagaagc ttatggcgcc tgacgaacgc gatcggagtt ttgacaaagc
     5761 gcttgcacgc catctgcgtt ccgccgcgcc tgcgggtgaa gctgcgggat tgcccgaaat
     5821 ccccgcctcg cggagcggcg catgccctga ttcggaaatg ctggcggcat atcacgagcg
     5881 gtcattgctt cccgaacaat tgaattcctt gaaagaacat atcgtcggct gcgccaactg
     5941 ccagaacgtc ctcgctcatc tggaaacgac ggacgaaatt tccttgcaag ccgctcagca
     6001 agacgaagtc ctcgctgtca aggaatccga gccggtgacg gctgcgcgga acctcgaatc
     6061 gttttccgca gcggcggctc ccggcaggag ccaaagcgct gccggagcag cactacccaa
     6121 gaggtcacgc cgccttcttc ttttgagggg cgcgcgctgg caatggctcg ctcccgcggg
     6181 agccatcgca gccggtctgc ttgtgtggat cgcgctgcac gaaaatcagc cgtcggcccc
     6241 tccaagtttg aagcaagtcc aagtcgcgac gaaccaggcg ccccctccac cggcaccatc
     6301 ggtttctaaa gcggtgcaag ggatctctcc gtcgccgagg gccgacctca cgaagcctca
     6361 atccgctgcc ggtggatatg ccggcgagaa tggtcgcggc gcttcgggcg ccacgaaatc
     6421 ggatgggaag caggaatatc tggcgcaggc attgccctcg aaaccgttgg tcgacaaaga
     6481 aggccgcttg cgaaaggaca cggcgcgcga ttcctccgtt gattcgcttc gtgccgagaa
     6541 tcaacgggat cgcgatacca agaccgtccc cggcgcgaga caagaaaacg ctgaagtgca
     6601 attacaggcg caagcggcga atgttcaatc gcagaatcaa tccaatgcca attcgccaaa
     6661 ggtggcgggt ccggcccccc tcggccagat ggaggcgaag aaaatgaagg ctgcctcacc
     6721 cgcgcccgcg cctccatctg ctgttggggg cgtcgcgggc tacaccggtg gggcttccat
     6781 gcaa
//
LOCUS       DASYVO010000110         2379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_142671, whole genome shotgun sequence.
ACCESSION   DASYVO010000110 DASYVO010000000
VERSION     DASYVO010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142671"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VGR03_03620"
     CDS             complement(<1..950)
                     /locus_tag="VGR03_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1993400.1"
                     /translation="MGTLGQDIRYALRNLRKAPGFALVAVITLALGIGASTAIFSVID
                     NILMEPFPYPDAQRYMSVQIHDTEQSEPGGRAGFIGPEFLDYVEQNHVFDRAIANDNT
                     DVLYRAGEGTQRFDGNFVTPGTFEFLGMPALVGRVMQSADYEPGAPPVFVLRYKTWVK
                     SFGADPGIVNKSFVLNGVSRTLIGIMPPRFEWGDADLWIPQKPSRAATSKVIAGAFQQ
                     HWFLLGHPKPGVSVKEAEADFTVVAKRLATVYPTEYPKHFTVQLESLTNLVVGRFKTT
                     LFIVLAAVGLLLLIGCGNVANLLLARATTREKEFAIRSALG"
     gene            1537..>2379
                     /locus_tag="VGR03_03625"
     CDS             1537..>2379
                     /locus_tag="VGR03_03625"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEV1993401.1"
                     /translation="MRILDRYIVREVFRHAFLGLVVFTFVFFVPQLVRLMELFVRHSG
                     SGAQVFKLFLCIFPAVFVFTVPMATLIGVLLGLGRMSADSEIIALTALGIGRRRILLP
                     VGVLALTGAVVTLAMTAWLGPLALRTFRSLQADLITSQVSFALQPRVFDERFPRLVLY
                     INDVTAAGTQWHGVFLAETGGEGGSRLTLAENAIVIAEPKQGKLELHLQGGTTHEFSR
                     EDADQYSVTAFGQSDWPIEFGGLAVAQPRQHNNPERSVRELWNDNGPGWREARVELHR
                     RFAFP"
BASE COUNT          445 a          719 c          686 g          529 t
ORIGIN      
        1 cccagtgccg accggatcgc gaattccttt tcccgtgtcg ttgcccgtgc aagcaacaga
       61 tttgccacgt ttccgcaccc gataagcagc agcagtccca ctgccgccaa gacaatgaag
      121 agcgtggtct tgaatcgacc gaccaccagg tttgtcagcg actccagttg caccgtaaag
      181 tgtttcgggt attccgtcgg gtacaccgtc gcaagccgct tggccaccac cgtaaagtcc
      241 gcttccgcct ccttcacgga gacacccggc ttcggatgcc ccaggaggaa ccagtgctgc
      301 tggaacgccc cagcgatgac cttacttgtc gccgctcggc tgggcttctg tggaatccac
      361 aggtccgcat cgccccattc aaatcgcggg ggcataattc caatcagcgt tctcgatact
      421 ccattcagca caaacgactt gttcacaatt cccggatcgg cgccgaaact cttcacccac
      481 gtcttgtaac gcaacacgaa caccggcggc gctcccggct cgtaatccgc cgactgcatc
      541 actcgcccca cgagcgccgg cattcccagg aactcaaacg ttcccggcgt cacaaaattc
      601 ccgtcgaagc gttgcgtccc ctctcccgcc cggtacaaca cgtccgtgtt gtcattggca
      661 atcgcgcggt caaacacatg attctgttcc acataatcca aaaactcggg gccgatgaac
      721 ccggctcgcc ccccgggttc gctctgttcc gtatcatgga tttgcacgga catgtaccgc
      781 tgcgcatccg ggtacggaaa gggctccatc aggatgttgt caatcacgct aaaaatcgcc
      841 gtggacgccc cgattcccaa tgccagcgtg atgactgcga ctaaggcaaa gcccggagcc
      901 ttccgcaagt tccgcagtgc gtatcggata tcctgcccaa gagttcccat gttcgcccct
      961 caattcaatt gactctgact cggctcgcct ggttctcagc ccgtagcggc ctgtctttga
     1021 aagcctgcct tgctgtctcg taagttacgc attcgtccgc aatttagttc gacgttgctc
     1081 cacggaataa gttgcctgca gtcccgggcc cgaacaattt agaagaaggc gtcggatgag
     1141 cctgcaatcc gttcatcggt ttttcataag cgattaagaa actatttaga tggaagccga
     1201 tcctgcgtta gcggagaagc tgccgcccgg gtgccgcatc gcaggtggag atacgcagtt
     1261 tgctcgtccg tgcctatgtg gtggccgaag tctagtggca cgaaaaaatt tggggtggga
     1321 gaagctgggc tcaagccagg cctcagcaaa ttttttgcag ggttaggatt gcgggcagat
     1381 ttggtgtgat tcggaactgt ttggagcccc agtcgcgcct gccttcgagg agcgggacca
     1441 aggtttggcc ggagaggatg agcgagtacg tgactcttgg cgaagagggg cgacaaggca
     1501 aacgttgtcg cggccgtgtt acactcacgg ttcctcatgc gtattctgga ccggtatatc
     1561 gtccgcgaag ttttccgcca cgcattcctc ggacttgtcg tcttcacctt tgtcttcttc
     1621 gttcctcagc tggtgcgctt gatggaactt tttgtgcgcc acagcggttc cggcgcgcag
     1681 gttttcaaac tctttctttg tatcttccct gcggtgtttg tgtttaccgt gccgatggct
     1741 acgctgatcg gtgtgctgct ggggctcggg cgaatgtcgg cggatagcga gataattgcg
     1801 ctgacggccc tgggcatcgg ccggcgccgg atcctgcttc ccgtcggcgt gctggcgctc
     1861 acgggcgcgg tagtgacgct ggcaatgacg gcctggcttg gaccgctggc gctgcggacg
     1921 tttcgttcgc tgcaggccga cctgatcacc tcgcaagtgt cctttgcgct gcagccgcgc
     1981 gtgtttgacg agcgcttccc gcggctggtt ctctacatca acgatgtgac agccgccggc
     2041 acgcaatggc acggcgtgtt tctggcggag acgggcggag agggtgggtc gcgactgacg
     2101 ctggcggaga acgcgattgt cattgccgaa ccgaagcagg gcaagttgga gctgcatctg
     2161 cagggcggca cgacgcacga attctcgcgc gaggatgcgg accagtactc cgttacggca
     2221 ttcggccaga gcgactggcc catcgagttc ggtgggctgg ctgttgccca accgcgccag
     2281 cacaacaatc ccgagcgttc ggttcgcgaa ctatggaacg ataacggccc gggttggcgc
     2341 gaagcccgcg tcgaacttca ccgccgtttt gcgtttccg
//
LOCUS       DASYVO010000111         7548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_30930, whole genome shotgun sequence.
ACCESSION   DASYVO010000111 DASYVO010000000
VERSION     DASYVO010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7548
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30930"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="VGR03_03630"
     CDS             complement(<1..1210)
                     /locus_tag="VGR03_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1993402.1"
                     /translation="MKRVLVCVILLAMPVRSVASPHAPRQAQVSRLSDNARVRAALDW
                     FTPNISWVNDQQARITEIPAPPFQEAQRAAAVKELLAEAGLAVQIDRTGNVIGELRGA
                     NEKEIVIVAAHLDTVFPPGTDVQVHRDGSRMAAPGISDNGSGVAALLALARAVQFAHL
                     KPQRTVLFVGDVGEEGEGNLRGMRAIVDAYRGKLKAVVVLDGSGTDHVTTKALASRRL
                     EALITGPGGHSWSDFGMPNPINALVRGSVRFINTKVPASPRTTFNIGQIEGGTSVNSV
                     PHEARLKVDIRSESEDELTRLESALRECIIAGVRDEMDSARDHSKGKLEWKVELLGSR
                     PGGELAPDSPLLAALRAADEIVGNQSRIERSSTDANIPLSLGIEAISIGAGGSGGGAH
                     SLQEWYDSAGR"
     gene            complement(1237..2472)
                     /locus_tag="VGR03_03635"
     CDS             complement(1237..2472)
                     /locus_tag="VGR03_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HEV1993403.1"
                     /translation="MQQNEWIYDWNVATPPAIPPGTRVLMNDETLRDGLQNPSVFDPS
                     IEEKIEILHLMESLGIDAVNIGLPGAGPRAFADTEALAREIATKRMKIKPNCAARTHE
                     NDIRPIVEISQRAGIAIEAATFLGSSPIRRLVEDWTVEHLERTTEKAVKFAVENGLPS
                     MYVTEDTVRTDPDTVKRLYSTAIRNGARAIVLCDTVGHATPNGAFNLVNFVVNEVVKP
                     SGERIRVDWHGHNDRGLSIANSLAAVAAGAHQVHAAANALGERVGNTPMELMLVNLRL
                     MGLIRQDLSRLKEYCEKVAKATHTMIPPNYPVVGHDAFRTATGVHAAALIKAFRKNDL
                     ELANAVYSGVPAHLFGLDQVIEIGPMSGKSNVLFWLEKHRIPADEATVTRIFDAAKQS
                     SRVLSDAEVLALCTEAARN"
     gene            complement(2505..2747)
                     /locus_tag="VGR03_03640"
     CDS             complement(2505..2747)
                     /locus_tag="VGR03_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993404.1"
                     /translation="MPEETATPPKIKTRKPKQRACDEKDEKGKLCNGHLKRYYDYPKE
                     VAALIGPKAEIYRCEFCKTLYRPDPVQQPQSYTLRN"
     gene            complement(2759..4330)
                     /locus_tag="VGR03_03645"
     CDS             complement(2759..4330)
                     /locus_tag="VGR03_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV1993405.1"
                     /translation="MAATAIPIDTLPSINPATGEVLAHFEKTSASLLQEIVSRARVAQ
                     GVWAKLPIRDRCAKVRGLRERMMASRNELADAVVRESGKPRVEALFADIFVALDSSEY
                     WSRNAASALRMRQVPHHSTAAKAKRGYLIYDPLGVIAIISSWNYPLAIPLSQIIPAVA
                     AGNAVVCKTSDFTPQCGALIEKLFIDAGFPGNLVTIVQGSGEVGQALIDASPDKVMFT
                     GSVATGRRVAEACAKKLIPSVLELGGKDAMIVLADADLEVASSAAVWGSYTNCGQVCL
                     SVERLFVEQDAAEKFMALCVEKTRKLRIGPGSEPSTDVGPLIRPQHVQRMSDLVRDAA
                     SRGAKVLCGGNARPELGPNFFEPTVIAGVDSSMRLFQEETFGPILAIQVVRDAKEAIT
                     QANDSPFALAASIWTKNKTRGISLAAELRAGAVMVNDAISYFGIAEAPHGGCGASGWG
                     RTHGQAGLLEMVQTKYIDVDRLPRREKPWWYRYGSELERAADAFLQFEFSGGIGAKLR
                     NARSAMKTFFRDHGL"
     gene            complement(4498..4884)
                     /locus_tag="VGR03_03650"
     CDS             complement(4498..4884)
                     /locus_tag="VGR03_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEV1993406.1"
                     /translation="MTRVLLDTHALVWWLEAKENLSRKAKEIIEDSETVVFVSAASGW
                     ELAIKTQIGKFKPAELVLGLEREIEKEGFIELPISMEHALVAGSLGSPHKDPFDRLLI
                     AQAQVEEVPIVSNDKVFDGFSVRRVW"
     gene            complement(4881..5111)
                     /locus_tag="VGR03_03655"
     CDS             complement(4881..5111)
                     /locus_tag="VGR03_03655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEV1993407.1"
                     /translation="MYTVHQAKTNLSKLLRKAARGEEVIIARGKTPVAKLVAVPHQKK
                     KRVPGRWKGKISYTPDAFAPLTKEELAEWGIE"
     gene            complement(5225..6019)
                     /gene="truA"
                     /locus_tag="VGR03_03660"
     CDS             complement(5225..6019)
                     /gene="truA"
                     /locus_tag="VGR03_03660"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV1993408.1"
                     /translation="MPGPRLGMRYLKLTIAYDGTDFHGWQIQSNKPTIQGEIVGVLRR
                     LTQEHVVLHAAGRTDAGVHALGQVASFRTQSGLSAQEFQRALNALLPPTIRIVGAGEV
                     GPDFNARWSARGKTYRYRLYRGKVVPPMVWRYVLHYPFPLDEDAMRDAAARFAGTHDF
                     ASFAASTGSEEDDKERSTEREIYSTELVRTADNEELVFTVRGRSFLRYMVRKMVGTLL
                     DVGRGKLAPADIDRLFELRDRSKSGPTVPSQGLVMVEVQHEEAWRL"
     gene            complement(6019..6636)
                     /gene="pyrE"
                     /locus_tag="VGR03_03665"
     CDS             complement(6019..6636)
                     /gene="pyrE"
                     /locus_tag="VGR03_03665"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV1993409.1"
                     /translation="MLKSEELLKLFETAGAIRHGHFELSSGLHSGTYVQCALVLRYPR
                     FAEKLGKALAALFSDARIDAVVSPAIGGLIIGQEVARALPEQKSSLGGGTPALFVERD
                     ASGMMTLRRGFSLAPDQHVLVVEDVWTTGGSTQEAIRVVEEAGARVVAAGALIDRSGG
                     KIDFEVEAQSLIQLPIASYEEEDCPLCRGGSVAVKPGSHFVRSAP"
     gene            6940..>7548
                     /locus_tag="VGR03_03670"
     CDS             6940..>7548
                     /locus_tag="VGR03_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993410.1"
                     /translation="MKTLYARPLLAAILAVTLAGGPISPAFGQDAPQNPQAAPAKTAT
                     PIVPVSLGSAKHNYSRSPRAFPNLFAPYSPIKIEEPGLTNSPRIDQLIHDGKLEISLQ
                     ETVELALENSMDIVVQRYNPWFADVGILKAKAGGFGGATPGAAFGGSNANTPLLNFDP
                     VITTTLSIDDRNIPVNNPLTSGTGTGLSSLARLTLHATTFNTQ"
BASE COUNT         1451 a         2313 c         2260 g         1524 t
ORIGIN      
        1 cgcggccggc ggaatcgtac cattcctgca gcgagtgcgc gccgccgccg ctgccgcccg
       61 cgccgatgga aatggcctcg ataccgaggg agagcggaat attcgcatct gttgaagagc
      121 gttcaatgcg cgattgattg ccgacaattt catcggcggc gcgaagtgcg gccagcagcg
      181 gcgaatccgg ggccaattcc ccgcccggac ggctgcccag cagttcgacc ttccattcca
      241 acttcccttt cgaatggtcg cgcgcggaat ccatctcgtc acgaacgccc gcgatgatgc
      301 actcgcgaag cgcggattcg aggcgtgtca gttcgtcctc gctctccgag cgaatgtcga
      361 ctttgaggcg cgcttcatgg ggaacggagt tcaccgaggt gccgccttcg atctggccga
      421 tgttgaacgt ggtgcgcggg ctggccggaa ctttggtgtt gatgaagcgc acggagccgc
      481 ggaccagcgc gttgatggga ttgggcatgc cgaagtcaga ccagctgtgg ccgccgggac
      541 cggtgattag ggcttcgagc cggcgcgaag ccagcgcttt cgtcgtcacg tgatcggtgc
      601 cggagccgtc aagcacgaca accgctttga gtttcccccg gtaggcatcc acaatcgcgc
      661 gcatgccgcg aagattgccc tcgccttctt cgccaacgtc gccgacgaaa agaacggtgc
      721 gctgaggctt gaggtgcgcg aactgcacgg cgcgcgccag cgccagtagc gctgccacgc
      781 cgctgccgtt atcggagatt ccgggcgcgg ccatgcggct gccatcgcgg tgaacttgca
      841 cgtccgtgcc gggaggaaaa acagtgtcga ggtgcgccgc aacgatgaca atttcttttt
      901 cattcgcgcc gcgcagttcc ccgatgacgt tgccggtcct gtcgatttgc accgccagcc
      961 cggcttcggc cagtaactct ttgaccgctg ctgcgcgctg cgcttcctga aaaggcggcg
     1021 ctgggatttc cgtgatgcgc gcctgctggt cgttgaccca ggaaatgttt ggagtgaacc
     1081 aatcgagagc ggcgcggact cgcgcgttgt cggaaagccg tgaaacctgg gcctggcgtg
     1141 gcgcatgggg ggaagcaacg gagcgaaccg gcatagccag aagaataacg caaacgagga
     1201 cgcgtttcac tgtcggcttg caacatcgtt ttccgctcaa ttgcgtgcag cctcggtgca
     1261 cagggcgaga acttccgcat cggataggac gcgcgaggac tgcttggcgg cgtcaaagat
     1321 gcgcgtgacc gtggcttcgt ccgcgggaat gcgatgcttc tccagccaga acagcacgtt
     1381 ggatttgccg ctcatcgggc caatctcaat cacctggtcg aggccgaaaa gatgcgcggg
     1441 cacgccgctg tagactgcat tggcgagctc gagatcattt tttcgaaacg ctttgatgag
     1501 cgcggcggcg tgaacgccag tcgcggtgcg aaacgcgtca tggccgacga ccggatagtt
     1561 cggcggaatc atcgtgtgcg tggccttggc gactttttcg caatactcct tgagccggga
     1621 gaggtcctgc cggatgagcc ccatgaggcg aaggttgacg agcattaatt ccattggcgt
     1681 gttgccgacg cgttcgccga gagcgttggc ggcagcgtgg acctggtgag caccggcagc
     1741 gacagccgcg agtgaattgg cgatggagag gccgcgatcg ttgtgcccat gccagtcgac
     1801 gcgaattctt tcgccggacg gtttcacaac ttcgttgact acgaaattca cgaggttaaa
     1861 tgcgccattt ggcgtggcgt ggccgacggt gtcgcagaga acgatggcgc gcgcgccgtt
     1921 tcgaatggcg gtcgagtaca gccgcttgac ggtatcggga tcggtgcgaa cggtgtcttc
     1981 cgtgacgtac atggacggaa gcccgttctc gaccgcgaac tttaccgctt tttcggtggt
     2041 gcgctcgagg tgctcgaccg tccagtcttc cacgaggcgg cggatcgggc tggagccgag
     2101 gaacgttgcg gcttcaatgg cgatgcccgc gcgttgcgaa atttccacaa tggggcgaat
     2161 gtcattttca tgcgtgcgcg ccgcgcagtt gggcttgatc ttcatccgct tcgtggcgat
     2221 ttcgcgcgcc agcgcttcag tgtccgcgaa ggcacgcggc ccggcgcccg gcagaccgat
     2281 attgaccgcg tcgattccca gggattccat cagatgcagg atttcgattt tttcttcgat
     2341 gctggggtcg aagacggagg gattctgcaa accatcgcgc agggtttcgt cattcatcaa
     2401 aacgcgcgtg ccgggaggaa tcgccggcgg cgtggcgacg ttccagtcgt agatccattc
     2461 gttctgctgc acggcgggcc tcgtttgctg cgaaatcgat agctctagtt gcgcaacgta
     2521 tagctctgcg gctgctgcac gggatccggg cgatacagcg tcttgcagaa ttcgcagcgg
     2581 taaatctccg ctttgggacc aatgagcgcc gcgacttcct tcgggtaatc gtagtagcgc
     2641 ttcaaatggc cgttacagag cttgcccttc tcatcttttt catcgcacgc ccgctgtttg
     2701 ggcttgcgcg ttttgatttt gggtggcgtt gcagtctctt cgggcatgaa gctcctctct
     2761 acaatccgtg gtcacggaaa aacgttttca tggcactgcg ggcgttgcgc aacttcgcgc
     2821 cgataccacc ggaaaattca aattggagaa aagcatccgc cgcgcgctcc agctccgagc
     2881 catagcggta ccaccagggc ttttcgcgcc gcggcaagcg gtcgacatca atgtatttcg
     2941 tttgaaccat ttcgagcagc cccgcctgcc catgcgtgcg tccccagccg ctcgcgccac
     3001 aaccgccgtg cggcgcttcc gcaatgccga aatagctgat ggcgtcgttc accatcaccg
     3061 caccggcgcg gagttccgcc gcaagggaga ttcctcgagt tttgttcttt gtccagatac
     3121 tcgccgcaag cgcgaacggc gaatcgttag cttgcgtaat cgcttccttc gcatccctca
     3181 cgacttgaat cgcgaggatt ggcccgaagg tctcttcttg aaacaatctc atggaggaat
     3241 cgacacccgc gatgaccgtg ggctcgaaga aattggggcc aagctcaggt cgcgcgttgc
     3301 cgccacacaa caccttcgcg ccgcgcgagg cggcatcgcg gactaaatcg ctcatgcgtt
     3361 gcacgtgctg cggccggata agcgggccga cgtcggtcga gggttcgctt ccgggaccga
     3421 tgcggagctt tctcgttttc tccacgcaga gcgccataaa cttttccgcc gcgtcttgct
     3481 cgacgaacag ccgctccacg gaaaggcaga cttggccgca gttcgtgtag ctgccccaga
     3541 cggccgcact ggaggcaacc tccaggtccg catccgccag cacgatcatg gcatctttgc
     3601 cgccaagctc gagcacggaa gggattagtt tttttgcgca agcttcggcg acgcgccgcc
     3661 cagtggccac gctgccagtg aacatgactt tgtccggcga ggcatcgatc aacgcctggc
     3721 cgacttctcc gctgccctgg acgatcgtga cgagatttcc cggaaaaccc gcgtcgatga
     3781 aaagtttttc gatcagcgcg ccgcattgcg gggtgaagtc gctcgtcttg cagaccacag
     3841 cattccctgc ggcgacggcc ggaatgatct gactgagcgg gatcgcgagc ggatagttcc
     3901 acgaagaaat gatggcgatg acaccgagag ggtcgtaaat cagatagccg cgtttggctt
     3961 tcgcggcggt gctgtgatgt gggacttgcc gcatgcgcag agcgctcgcc gcgttcctgg
     4021 accaatattc cgaggaatcg agagccacga aaatatccgc aaagagtgct tccacgcggg
     4081 gcttgccgga ttcgcgcaca acggcgtccg ccaattcgtt gcgcgaagcc atcatgcgtt
     4141 cgcgaaggcc gcgcaccttc gcgcatcgat cgcggatcgg aagcttcgcc cagacgccct
     4201 gggcgacccg cgcacgggaa acgatctcct gcagcagcga cgccgaagtt ttctcaaaat
     4261 gcgcgagcac ttccccggtg gccgggttga tcgaaggaag cgtgtcgatg ggaatggccg
     4321 tggccgccat atccgcgatt ctaaccgatt ttcggctagg atgtggacgt ggaatctctg
     4381 ccccccgccc gcctccttgc gccgcttcag aaatcagatt ccgtttaatg gtctgcttgg
     4441 acagagaaga gttgcaggtt ctcggccttc tagcgcatca cggcgggaat aatccagcta
     4501 ccaaacccgg cgaacggaga acccgtcaaa caccttatca ttgctgacga tcgggacttc
     4561 ttcgacttgg gcttgagcga tcaacaggcg gtcgaacggg tctttgtgtg gtgagccaag
     4621 gctacccgcc actagggcgt gctccatgga aatgggtagc tcgatgaagc cttccttctc
     4681 aatctccctc tcgaggccaa gcacaagttc ggctggcttg aatttgccaa tttgcgtctt
     4741 gatcgccagt tcccagcccg atgcggcaga aacgaacacg accgtctctg aatcttcgat
     4801 aatctccttg gctttgcggg aaagattctc ctttgcttcg agccaccaca ccaaagcgtg
     4861 tgtgtccaga agcactcgcg tcattcgatg ccccattcgg ctaactcttc tttggtgagt
     4921 ggcgcaaagg catcaggagt gtaagaaatc tttcctttcc atcggcccgg gacgcgcttc
     4981 tttttctgat gaggcacagc cacgagcttg gcgaccggcg tcttaccgcg ggcaataatg
     5041 acctcctcgc cccgggcggc cttgcgaaga agcttcgaga ggttggtctt ggcctgatgg
     5101 acggtataca tggttagcta agtatattag ctaacctcgc cgccggcaag atcttttgcc
     5161 cgggccggtg aagacgggac gcaaacggag cctggcagtc agccggtcga acagcgcggc
     5221 tcgctcagag gcgccaggct tcttcgtgct ggacttcgac cataacgagg ccttgcgatg
     5281 gtacggtagg cccggacttg gaacggtccc gcagttcgaa caagcgatcg atgtccgcag
     5341 gcgcgagttt tccgcgcccc acgtcaagca gcgtgcccac catcttgcgc accatataac
     5401 gaaggaacga gcgcccgcga acggtgaaca ccagctcttc gttgtccgcc gtgcgcacca
     5461 actcggttga gtagatttcg cgctcggtgg agcgctcttt gtcgtcctct tcggagcctg
     5521 tcgaagccgc aaaagaagca aagtcgtgcg tgccagcaaa ccgagccgcg gcgtcgcgca
     5581 tggcgtcttc atccagcgga aacggatagt gcaggacgta gcgccagacc atcggcggca
     5641 cgactttccc gcgatacagc cgatatcggt aggtcttgcc gcgtgccgac cagcgcgcat
     5701 tgaagtccgg tcctacttct ccagcgccga cgattcgaat tgtaggcgga agcagagcgt
     5761 tcagagcgcg ctgaaattcc tgcgccgaga gcccggactg cgttcgaaag ctcgcgacct
     5821 gaccaagcgc gtgaaccccg gcgtcggtgc gtccggcagc atgcaaaacg acgtgctcct
     5881 gcgtcagtcg ccggagcacg ccaacaattt ctccctgaat cgtgggcttg ttggattgaa
     5941 tctgccagcc gtggaagtct gtgccgtcgt acgcgatggt aagcttgaga taacgcatgc
     6001 cgagccgagg gccgggcatc aaggcgcgct gcgaacgaag tggctccccg gcttcaccgc
     6061 cacgctgccg ccgcggcaga gcgggcagtc ttcttcctcg tagctggcga tgggcagttg
     6121 gatcagcgac tgcgcttcca cttcgaagtc aatctttccg ccgctccgat cgatcagcgc
     6181 gccggcagca acgacgcgcg cgccagcctc ttccacaacg cgaatggctt cctgcgtcga
     6241 gcccccggtg gtccacacgt cctcaacgac aagaacatgc tggtcgggcg cgagagaaaa
     6301 gccacgccgc aacgtcatca tgccgctggc atcgcgttcc acaaaaagcg caggcgttcc
     6361 cccacccagc gaactcttct gctcaggcag cgcccgggcg acttcctgcc cgatgatgag
     6421 ccccccgatg gccggtgaga cgacggcgtc gatccgggca tctgaaaaga gggcggccag
     6481 cgcctttccc agcttttcgg cgaagcgcgg ataccgcagg acaagcgcgc actgaacgta
     6541 cgttccgctg tgcagcccgc tggacaattc aaagtgccca tggcggatgg cgcccgccgt
     6601 ctcaaacagc ttcaacaatt cttcgctttt cagcattccg ctcagctaac cgctgccagc
     6661 tcctaaccgt cgaaatgcca gcagcctagc cgccgcccac cgcgttgtca aggaagaggg
     6721 cccctgctag actgctttct tcgcccggtc ggcgcggcgc agccttcggg atggcttttg
     6781 gaccgtccct ggcggggtga ccgcgaggct gtcagatttc ggtcgtgacc tcaaaacggt
     6841 caacttcttg aaacaaattc cattctgcga acgttttaca gagttgagta catataaatt
     6901 aagccccttg ggggatgaat cttgagggta aggagcacta tgaagacgct gtatgctcga
     6961 ccactgttgg ccgctattct ggccgtaacg ctcgcgggag gacccatttc cccggccttc
     7021 gggcaggatg cgccgcaaaa tccgcaagca gcgcccgcca agacggccac gcccatcgtg
     7081 ccggtttcgc ttggctccgc gaagcacaac tatagtcgtt cgcctagggc ttttccgaac
     7141 ctctttgctc cctacagccc catcaagatc gaagagcccg gcctcaccaa ctcgccccgc
     7201 atcgaccagc tcatccacga cggcaagctg gagatcagcc tccaagagac ggtggagttg
     7261 gcgctggaaa acagcatgga catcgtggtg cagcgttata acccctggtt cgcggatgtc
     7321 ggcatcctca aagcgaaagc cggaggcttt ggcggcgcca ctcctggggc cgcgtttggc
     7381 ggttcaaatg ccaacactcc cctgctgaac ttcgatcccg tcatcaccac gaccctcagc
     7441 atcgacgacc gcaacatacc ggtcaacaac cctctcactt ccggtaccgg cacagggctc
     7501 tcttcgctcg ccagactgac gttgcacgct acgactttta atacccag
//
LOCUS       DASYVO010000112        11747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_271098, whole genome shotgun sequence.
ACCESSION   DASYVO010000112 DASYVO010000000
VERSION     DASYVO010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 11747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11747
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271098"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VGR03_03675"
     CDS             complement(<1..191)
                     /locus_tag="VGR03_03675"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEV1993411.1"
                     /translation="MENRDVARILRETAQLLEIDGAFIARYRSYEKAAELVEGLAERI
                     QDLATDPARLTALPGIGDRL"
     gene            complement(376..852)
                     /locus_tag="VGR03_03680"
     CDS             complement(376..852)
                     /locus_tag="VGR03_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993412.1"
                     /translation="MHRGLATLSGAVMICFLLLTQADMNFFHLQLLQSAIYLMVILML
                     FYFEDHWAYMLGMIAPTVWLLLAFGVGTLGGGLRAAGLLVSGRTRVDELSLLDFITAG
                     FCVLLIVFSARRWRKEYADSHKGLPTFVVTFAGVAIYFAMLIYFYWRGIPHGPLAG"
     gene            complement(1072..1614)
                     /locus_tag="VGR03_03685"
     CDS             complement(1072..1614)
                     /locus_tag="VGR03_03685"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1993413.1"
                     /translation="MRAAKARVIHSKMLYQGRVFGVRRDRVIEPGGVEVTRDVVTHNG
                     SVVLVPVFPDGSILLVRQYRHPVERFLWELVAGRVERGEAPGIAAKRELQEETGYTAK
                     RFRKLMDVFPTPGFVSENMVVFLAEGLTAGAARPEADEKITAKRFSLARLERMMRTGA
                     LRDAKSIAAILFYARFVRRG"
     gene            complement(1661..2455)
                     /locus_tag="VGR03_03690"
     CDS             complement(1661..2455)
                     /locus_tag="VGR03_03690"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HEV1993414.1"
                     /translation="MDPKTFTREIETHVRPATFPVAAKLLRPDEPVPEKAKRPHRDFK
                     IQIAICQAIAMARRYGWTVAVGEEDVSCVLTKTVFGLAPLTEHFTSGHLACGMYAETP
                     AAAVRTEAATERLPEGKYKHLLVAPAARADFEPDAVVIYGNPAKVMRLVTAALWKRGG
                     ALSSSFTGRIDCSDEIIRPMLSGDYQVILPCYGDRVFAQTDDGEMAFSLPGAKMEELV
                     EGLAGTHKGGIRYPIPTFLRYTPQYPEHYYELEKIWAAGEIGKSST"
     gene            complement(2520..5636)
                     /locus_tag="VGR03_03695"
     CDS             complement(2520..5636)
                     /locus_tag="VGR03_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEV1993415.1"
                     /translation="MNDEPKKTSGFLHRTWTTTTEWLRGLGHQKREDPWAAKSFDQIQ
                     RDEWWEIRKARDLRRGQANTNPPEQDVPKPITQQNLVGLAFSGGGIRSATLNLGILQG
                     LAKFNLLQTFDYLSGVSGGNYIASWLAAWIKREGFTEVSGKLSPDRANPPRDEEAPAI
                     RWLREYSNYLTPRMGLASGDAWAAMATYFRNLLLNQTILVLVFGGVLLLPRVLTWAAR
                     EIGESWDTGLFQIPWDSVLLSWAPHSLIGLIGPWVGQGHFYFVGAFLLLTLGVRQIHN
                     NMTWFSSAKEAASNAGQVFLTIVVPFIVASWALTVWLWYGPAADKQPWLAPSDGAWWH
                     WMTLIALLYTIPWLLASWGNTFGPAILGLLIRSKSKRKYVIPITAPFAGAFGGILLWR
                     IVKLFHGWNILSRTESSGGLWQAMSWGTPLVLLTMTLMAVLHIGLMGTGFQEGKREWW
                     SRLGGYMLACGLAWATLFGFSVYGPMVVMWMGGKLATGSLVGWIATTAGGVLGGKSAK
                     TDKNPSNSWLGLFLKAAPVVFIAGLLLLVSFGVHVAVVKVSQPDFSLNNIWHHFQWGT
                     AKDTSKVEVKVYIDCPSGEKEIVCAPGEEKEKKKAEESVVSLHWKLMNGSLGGCNLAE
                     MLLAFLGVFLLAWRVDINEFSMHLFYRNRLARCYLGASRKEREPTTNPFTGFSPKDDL
                     FLATLSNAPAMKDRLRGMDDGKPDYAGPYLLVNTALNLTSGQKLAWQSRKARSFTFSP
                     RYCGFEVLKDDPRRPQLEEHGYRETLVYAFFQLFSLGGPLTGTAMGISGAAASPNMGY
                     HSSAPLAFLMTVFNVRLGMWLGNPRHRKAWQRYGPKLGLPYLFNELAGMATDERKYVY
                     LSDGGHFENLGVYELVRRRCKYIVACDASADPKVSFDDLGNAIEKCRVDFGVDIEIDV
                     DNLKPPKDSRISRWHCAVGRVRYKQAFSNEEDGIIVYLKSSLTGDEPADVRHYSEQNT
                     AFPHTSTGNQFFDESQFESYRELGYHIITTTFGALGDPQKLGDKGPQEIFEDMQQRWR
                     RTPDTAGA"
     gene            5760..6725
                     /locus_tag="VGR03_03700"
     CDS             5760..6725
                     /locus_tag="VGR03_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV1993416.1"
                     /translation="MRPWGIAFPTWPVDASAASPFLDTRHGLSYTGCGSGAAPPSAAA
                     ARLLRTMPINTLIVDDEKPAREELAFLLKAFPEIILIAQGRNGLEAVALIKEHAPDLV
                     FLDVQMPGLDGFGVIKKLLDRKLRLPQIVFATAFDTYAVQAFEVNAVDYILKPFDKGR
                     VARAIQRAKKQLDAQASPVERLETLITQMGGPPGGPSGAGSAAASAAGRQTTKLLLRA
                     QQRMLLVDSGDLVYATINDGCISMSTRDIEGTSNYRTLEELQAQLDAEVFWRVHRSWL
                     VNINHIKEIVPWFKSSYVLRMNDKKQSEVPVSRTQTRRLRELLKL"
     gene            6901..8085
                     /locus_tag="VGR03_03705"
     CDS             6901..8085
                     /locus_tag="VGR03_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV1993417.1"
                     /translation="MNEQIKQWREDTPGCRHRNHLNNASAGLMPRSVLDAIVEHLQRE
                     SEFGGYESADAAADAFQASYDSVAQLIGASPRNIAIVENSTVAFFQALAAFDFTPGDV
                     IVTTRNDYISNQLAYLSLGRRHGVEVRRAADLSTGGVDPQSVRELLRQPRTRLLAATW
                     VPTNSGLIQPVEALGEIAEQAGVPYLIDACQAAGQIPINVQRLRCDFLAATARKFLRG
                     PRGIGFLYVSDRALKRGDFPLYIDMRGADWVTADTFQFAPDARRFENWEFAYSLVLGL
                     GAAARYAIAVDVERGGRRARELAAALRSKLEVVPGLRVLDRGADLAALVTVEVAGRDA
                     SEIVPLLHRRGINTSVTSRRYAVIDMDDKHAVSAVRISPHYYNTEDEIAAVVDALKSL
                     PA"
     gene            complement(8094..8537)
                     /locus_tag="VGR03_03710"
     CDS             complement(8094..8537)
                     /locus_tag="VGR03_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993418.1"
                     /translation="MYQVKVTKSLRFPDGTTAREESHTEKVNHERHALNLAIKIARQE
                     NVEWVEIEDVKGRFKARAAHAADGTVHKSLESRRSNRYRKMRFGRRRNSSVGCLVLIC
                     LVVLGVLGRAASMPAPSQDTGFVAKLIVVVLAAPVFVWIYLRKRR"
     gene            9646..10905
                     /locus_tag="VGR03_03715"
     CDS             9646..10905
                     /locus_tag="VGR03_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV1993419.1"
                     /translation="MDFSLTDDQRLLKETIRHFMEAEIRPILREYEREERFPLEQIRK
                     LGELGCCGIMVPEELGGSGSDAVTYVVMLEEVARVCASTAVTLGVTNSAVCSPLSRHG
                     TTAQKEKYLRRLASGEILGSFCLTEPGAGSDAAAITTRAVRTAGGYRLTGTKTWVSTG
                     GVSGIYLVFAKTDPAAGARGVSAFLVEPEFRGFRVSRYEDKMGLRMSRTAEITFDECE
                     VPDENRLGEEGQGLKVALETLDGGRIGIAAQAVGVAQGALEASVRYAKQRRAFGKTIG
                     DFQAIQWMLADMQTEIEAARGLTYYAAWRYDQDARRASSAGPSAGPFKEKTRAGADAA
                     RAKLYSSEMVNRVVYKAVQIHGSMGYSRETDVERMYRDARVITIYEGTSEIQRMVIAR
                     DLLSRGAVSSNDSRQGAASAVPRGAAE"
     gene            10902..>11747
                     /locus_tag="VGR03_03720"
     CDS             10902..>11747
                     /locus_tag="VGR03_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993420.1"
                     /translation="MIATSAPRKRVVSGMRPTGRLHIGHYFGALANWIKLQNDPAYEC
                     FYFIADWHALTSDYADTSQVSQNTIEIATDWLASGLDPAKSTFFVQSQVPEHAELHLL
                     LSMVTPLGRLYSLPSYKEQLENIKDKNLQTYGFLGYPVLQCADIVIYGEPKAPLLVPV
                     GEDQVSHVELSREIVRRVNIFYGLDVDPKLFLRENEHALRQIASHSNAHLPSDWKYPV
                     EPGVQTFLIGRFRSAAAEEGIDNFLKVVGNLGNFVRWDEVLKEPGVMLTKTPRIPGTD
                     GRKMSK"
BASE COUNT         2310 a         3909 c         3184 g         2344 t
ORIGIN      
        1 agccggtcgc cgatccccgg tagcgccgtc agccgcgccg ggtccgtcgc caggtcctgg
       61 atgcgctcgg ccaatccctc gaccagctcg gcggcctttt catagctgcg gtagcgcgcg
      121 atgaacgcgc catcgatctc cagtagttgc gcggtctcgc gcaggatgcg ggctacgtcg
      181 cggttctcca tggcagggaa attatacgcg atgccccctc atccgcgagt cccgtaaacc
      241 ttgtgccaac taaaccgctc agcgctcttg gccgggcgcc cggccaagcg gttctcatgc
      301 gggtgtcgga ggcggcaaga atccgcacgc cgcggttgtg gagagatcgc aatgcggtgc
      361 ccggatggtt tggtcttatc cggccagcgg gccgtgcggg atgcctctcc agtagaagta
      421 gatcagcatt gcgaagtaaa tcgccacgcc ggcaaaggtc accacaaacg tcggcaaccc
      481 cttgtggctg tcggcgtatt cttttcgcca gcggcgcgcg gagaagacga tcaaaagtac
      541 gcagaagccc gcggtaatga agtccagcag gctgagctca tcgacgcggg tgcgcccgct
      601 gaccagcagt cccgccgcgc gtaagccgcc tcccagcgtg cccaccccga aggccagcag
      661 cagccacacc gtgggggcga tcatccccag catgtaggcc cagtggtcct caaagtaaaa
      721 cagcatgagg atgaccatca ggtagatcgc cgactgcagc agttgcagat ggaaaaaatt
      781 catgtccgcc tgcgtcagca gcaggaagca gatcatcacc gctccggaaa gggtcgcgag
      841 tcctcgatgc atatcttgca ctcccccggt ccagattcgg gtcagctttg ggccagcgtc
      901 gggcgagacg ttactcgcct gcggccagcg aagtcaagca ccccccatct ccactgcacg
      961 tcattctgag cccgccgcgg cgggcgaaga atccctctgg ttcctcgtct ccgccgctct
     1021 tttccttgct ctccttcact ctggcgccat catctcactg acccaccggc ctcacccgcg
     1081 ccggacaaat cgcgcgtaaa acaatatcgc cgcgattgac ttcgcgtccc gcagcgcgcc
     1141 cgtgcgcatc atccgctcga gccgcgccag cgaaaatcgc ttggccgtaa ttttttcatc
     1201 cgcctccgga cgcgccgccc ctgccgtcaa tccctcggcg agaaacacca ccatgttttc
     1261 actcacaaat cccggcgtcg gaaagacatc catcaacttg cgaaatcgct tcgccgtgta
     1321 cccggtctcc tcctgcagct cccgcttcgc tgcgattcca ggcgcttcgc ctcgctccac
     1381 gcgccccgcc accagctccc acaaaaaccg ttccaccgga tggcgatact gccgcaccag
     1441 caaaatgctc ccatcgggga acaccggcac cagcaccacc gaaccgttat gcgtgacaac
     1501 gtcgcgcgtc acctcaacgc cgcccggctc gatcacgcga tcccgccgca ctccaaacac
     1561 tcgcccctga tacagcatct tgctgtggat gacccgcgct ttcgctgctc tcaagtctcc
     1621 ctcttctctg tgtcctctgt gttaattctt tcttcgcttt tcacgtcgaa ctcttgccga
     1681 tctctcccgc cgcccagatc ttctccagct cgtaataatg ctccggatac tgcggcgtgt
     1741 acctcagaaa cgtgggtatc ggatatcgaa tcccgccctt gtgcgttccc gccagccctt
     1801 ccaccagctc ctccatcttc gcccccggca acgaaaacgc catctcccca tcatcggtct
     1861 gcgcgaacac gcgatccccg tagcagggca ggatcacctg gtaatctccg gacagcatcg
     1921 gccggataat ctcatccgag cagtcgatcc gcccggtaaa cgaactgctc agcgcccctc
     1981 cccgtttcca cagcgccgcc gtcaccagac gcatcacctt cgccgggttt ccgtagatca
     2041 ccacggcgtc gggctcgaaa tccgcccgcg ccgccggcgc caccagcagg tgtttgtatt
     2101 ttccttccgg caggcgctcc gtcgccgctt cggtgcgcac cgccgccgcc ggcgtctccg
     2161 catacatccc gcaggccaga tgcccggacg taaaatgttc ggtcagcggc gcgagtccaa
     2221 agaccgtctt ggtcaggacg caactcacgt cctcttcacc caccgccacc gtccagccgt
     2281 agcgacgcgc catggcaatc gcctggcaga tggcaatctg aatcttgaaa tcccggtgcg
     2341 ggcgcttggc cttctccggc acgggctcat cgggccgcag cagcttggcc gccacgggaa
     2401 atgtcgccgg ccgcacgtgc gtttcaattt cgcgggtaaa cgtttttggg tccactggag
     2461 gcctccccgg tcaggatacc gcgaagcgct tcggcagcac aggcgctctc ggcgcgcggc
     2521 tacgccccgg ccgtgtcggg ggtcctacgc caccgctgct gcatatcctc aaagatttcc
     2581 tgcggtccct tgtcgccaag tttttgggga tcacccaagg ccccaaaggt ggtggtgatg
     2641 atgtgatatc ccagctcgcg ataactttcg aattgcgact catcgaaaaa ttggttgccg
     2701 gtggaggtgt gtgggaacgc ggtgttctgt tcggaataat ggcgcacgtc ggccggttcg
     2761 tcgccggtga gcgaggactt caggtatacg atgattccat cctcttcgtt cgaaaacgcc
     2821 tgcttgtagc gcacccgccc cacggcgcag tgccagcggc ttatgcgtga atctttcggc
     2881 ggtttcaggt tgtccacgtc gatttcgatg tccacgccga aatcgacgcg gcatttctcg
     2941 atggcattgc cgagatcgtc gaagctcact tttgggtccg ccgaggcgtc acaagccaca
     3001 atgtacttgc agcgccggcg aaccagctcg taaacgccca ggttttcgaa atggcccccg
     3061 tcggagagat agacatactt gcgttcgtcg gtggccattc cggccagttc gttaaacaga
     3121 tacggtaggc ccaattttgg cccgtagcgc tgccatgcct tgcggtggcg tgggttgccc
     3181 agccacattc ccaggcgcac gttgaatacg gtcatcaaaa acgccagtgg ggccgaggag
     3241 tggtatccca tattcggtga tgccgccgcc ccggaaatgc ccatggccgt gccggtgagc
     3301 ggccccccca aggagaacag ttgaaagaac gcgtacacca atgtctcgcg atagccgtgc
     3361 tcctccagtt gcggcctcct cgggtcgtcc ttgagcactt cgaatccgca gtatcgcgga
     3421 gaaaacgtga acgatcgggc cttgcgcgat tgccaggcca gtttttgccc cgaagtcaaa
     3481 ttcaacgccg tgttcaccag cagatacggc ccggcatagt cgggcttgcc gtcatccatt
     3541 cccctgagcc ggtctttcat tgccggcgcg ttcgaaagcg tggccagaaa caggtcatcc
     3601 ttcggggaga atcctgtaaa cgggttggtg gttggctcgc gttccttccg cgacgccccc
     3661 aggtaacagc gcgccagccg attgcgataa aacaggtgca ttgagaactc attgatgtcc
     3721 acgcgccagg caagaagaaa cacaccaaga aaagccagca gcatttccgc cagattgcac
     3781 ccgccgagac tcccattcat caatttccaa tgcagtgaaa cgacagattc ttccgctttc
     3841 ttcttctctt tctcctcgcc cggcgcacaa acaatttctt tttctccgga ggggcaatct
     3901 atgtacacct tcacttctac tttcgaggtg tcttttgccg ttccccactg gaagtgatgc
     3961 cagatattgt tcaaactaaa atccggctga gacaccttca ccactgcgac atgaacaccg
     4021 aacgacacca gcagcaacag gccggcaata aaaaccaccg gtgccgcctt caagaacagc
     4081 ccgagccagg agttcgaagg gtttttgtcc gtcttggcgc ttttcccgcc aagcactccc
     4141 cctgccgtcg tcgcgatcca tccaaccaga ctgccagtgg ccagttttcc acccatccac
     4201 atcaccacca tcgggccgta cacgctgaat ccgaaaagcg tcgcccacgc caatccgcaa
     4261 gcgagcatat agccgcccag ccgcgaccac cactcacgct ttccttcttg aaaccctgtt
     4321 cccatcaggc cgatgtgcag caccgccatc aaggtcatcg tcagcagcac cagcggagtg
     4381 ccccagctca tcgcctgcca caggcccccc gatgattccg tccgcgacaa tatattccaa
     4441 ccgtggaaaa gcttcacgat cctccaaagc aggattcctc cgaacgcgcc tgcgaacgga
     4501 gcagtaatgg gaatcacgta cttccgcttg gatttcgaac ggatcagcaa gcccagaatt
     4561 gcgggtccga aggtgttgcc ccatgacgcc aacagccagg gaatggtata cagcaacgcg
     4621 atcagcgtca tccagtgcca ccacgccccg tcggacgggg ccagccacgg ctgtttgtcg
     4681 gcagcgggtc cgtaccacag ccacacggtc agcgcccagg atgcaacgat gaaaggaaca
     4741 acaatggtca agaacacctg cccagcgttc gaggccgcct cttttgcgga ggaaaaccat
     4801 gtcatattgt tgtgaatctg ccgcacgccg agagtcagca acagaaatgc tcccacgaaa
     4861 tagaagtgtc cttgtcccac ccacgggccg atcaagccaa taagagaatg gggagcccac
     4921 gagagcaaaa cggagtccca gggtatttga aatagaccgg tgtcccagct ctctccgatt
     4981 tcgcgcgcgg cccaggtgag cacgcgaggg agcagcagaa cgcccccgaa caccagcact
     5041 agaattgtct ggttgagtag aagattgcga aagtatgtgg ccattgctgc ccacgcgtcg
     5101 ccgctcgcta ggcccattcg tggcgtcagg taattgctgt attcccgcag ccagcggatc
     5161 gccggagctt cttcgtctcg aggcggattt gcacggtcag gagagagctt accggacacc
     5221 tcggtaaagc cctcccgctt gatccaggcc gccagccaac tggcgatgta attgccgccg
     5281 gaaacgccgg aaagataatc aaatgtctga agcagattga atttcgcgag tccctgcagg
     5341 attccaagat ttaatgtcgc gctgcggatt ccgcctccgg aaaacgccag ccccaccagg
     5401 ttctgctggg tgatgggttt tggaacgtcc tgctccggcg gattggtgtt cgcttgaccg
     5461 cggcgcaggt cgcgcgcctt tcgaatctcc caccattcat cgcgttggat ttggtcaaac
     5521 gatttcgccg cccaggggtc ttctctcttc tggtgtccca gtccccgtaa ccattctgtg
     5581 gttgtcgtcc acgttcggtg caggaagccg ctcgtctttt tgggctcgtc gttcatgctg
     5641 cctcccgatc cacgcccgaa gggcgtgaaa taatctgcga tagggcgtca ccgtgtggtt
     5701 aaagcctgct gtccgcgcac gctagttctg atactgctaa tcgtcaagtg aagatatgcg
     5761 tgcgcccgtg gggcatcgcc ttcccgacct ggcccgtgga tgccagcgcc gcttcaccat
     5821 tccttgatac ccgccacggc ctttcctata ctggctgcgg cagcggagct gcgccgccat
     5881 ccgcagcggc tgcgcgcttg ctacgaacca tgcccatcaa cacgctcatc gtcgacgacg
     5941 aaaaacccgc gcgcgaggaa ctcgccttcc tgctcaaggc cttccccgaa atcattctca
     6001 tcgcccaggg ccgcaacggc ctcgaagccg tcgccctcat caaggagcac gctcccgatc
     6061 tcgtcttcct cgacgtacag atgcccggcc tcgacggctt cggcgtcatc aagaaactgc
     6121 tcgatcgcaa gctgcgcctt ccgcagatcg ttttcgccac cgccttcgac acttatgcgg
     6181 tgcaggcctt cgaagtgaac gccgtcgact acattctcaa gcccttcgac aaaggccgcg
     6241 tggcccgcgc catccagcgg gcgaagaagc agctcgatgc gcaggcctcg cccgtcgagc
     6301 gtctcgaaac cttgatcacg caaatgggcg gcccgccggg cggcccatcc ggagcgggtt
     6361 ccgccgcggc ttcagccgcc ggtcgccaga ccaccaagct tctcctgcgc gcgcagcagc
     6421 gcatgttgct ggtcgattcc ggcgacctgg tttacgccac tatcaacgat ggctgcattt
     6481 ccatgtccac ccgcgacatc gagggcactt ccaactaccg caccctcgaa gagctccagg
     6541 cccagctcga tgccgaagtt ttctggcgcg tccatcgctc ctggctggtg aacatcaatc
     6601 acatcaagga aatcgttccc tggtttaagt ccagctacgt tctgcgcatg aacgacaaga
     6661 aacagagcga agttcccgtc agccgcacgc aaacgcgccg cctccgcgaa cttctcaagc
     6721 tgtaggggcg gggcttgccc cgccctgtcc gcaggtcggc aatcccacgc cctctctttt
     6781 tccgcgtcct ctctccgtct cttctttccg ccccttctct ccgcctctgt gcgctccgtg
     6841 tctctgtgtt aatctttttc cgtctgcatg agcttgcgca ccgccgggcg aggagagcga
     6901 atgaacgaac aaatcaagca atggcgtgaa gacacgcccg gctgccggca ccgcaaccac
     6961 ctgaataacg ccagcgccgg cttgatgccg cggtccgttc tcgacgccat cgtcgagcat
     7021 ctccagcgcg aatccgagtt cggcggttat gagagcgctg acgccgctgc ggacgcattt
     7081 caggcatcct acgacagcgt tgcgcagctc atcggcgcca gcccccggaa catcgccatc
     7141 gtcgagaact cgacggtggc gttttttcag gcgctcgcgg ctttcgattt cacccccggc
     7201 gatgtcatcg tcaccacgcg caacgactac atctccaacc agctcgctta tctctctctg
     7261 gggcggcgcc acggagtgga agtgcgccgc gcagccgatc tttccaccgg cggcgtcgat
     7321 ccgcagtcgg tccgcgaact tctgcgtcaa ccgcgcacgc gcctgctggc cgccacctgg
     7381 gtacccacca actccggcct catccagccc gtcgaggccc tcggcgagat tgcggaacag
     7441 gccggcgtcc cttacctcat cgacgcctgc caggccgccg ggcaaatccc catcaacgtg
     7501 caacgcttgc gctgcgattt tcttgccgcc acggctcgta agttcctgcg cggaccgcgg
     7561 ggaatcggat tcctctacgt ttccgaccgc gcgctgaagc gcggagattt tcctctgtac
     7621 atcgacatgc gcggcgccga ttgggtcacc gcggacactt ttcagttcgc tccggacgca
     7681 cgccgattcg agaattggga gtttgcctac agcctcgtcc tcggcctcgg agccgccgct
     7741 cgctatgcga ttgccgtgga tgtcgagcgc ggcggccgcc gggcgcgcga attggcggca
     7801 gcgcttcgca gcaagttgga agtcgtgccc ggcctccgcg tgctcgatcg cggcgcggac
     7861 ctcgccgccc tcgtcaccgt ggaggttgcc ggccgcgatg cctccgagat cgtcccgctg
     7921 ctccaccgcc gcggcatcaa taccagcgta acctcgcgcc gctacgccgt catcgacatg
     7981 gacgataagc acgccgtatc cgccgtgcgc atctccccgc actactacaa caccgaagac
     8041 gaaatcgctg ccgtggtcga cgcgctcaag tccctaccgg catagtttcc gcctcaccgc
     8101 cgcttgcgga gatagatcca aacgaatact ggagcggcca gaacaacgac gataagtttt
     8161 gcgacgaaac ccgtatcctg agagggggcc ggcatggatg ccgcacggcc gagcacgccc
     8221 agaacgacca aacaaatcag gaccagacag ccaacggaac tgttccttcg ccgcccgaag
     8281 cgcatcttcc ggtatctgtt ggaacggcga gattctaagg atttgtggac tgtcccgtcg
     8341 gccgcgtggg cggcacgagc tttgaagcgg ccttttacgt cctcgatctc cacccactcc
     8401 acgttttcct gccgggcaat ctttatagcg agatttagcg cgtgccgttc atgattgact
     8461 ttctctgtat gcgactcttc gcgggcggta gtcccatcgg ggaatcgaag tgattttgta
     8521 actttgactt gatacatcac cgaccccaag gacaatcggt tgagtttcgg cggttagtaa
     8581 atgacggccg attgggcgat ttccgtcaac ggcaactcct gatttccagc atgttccgaa
     8641 acctatcacc taatacgcat aactggtagc atcctaatat gatttctcca ggcaaggcca
     8701 tgtgatagga tgcgaacatg caacccaatt tacatgggca atccgaaatc gacgggttcc
     8761 ctcaactctc cgctaaagag tgggcgctgc gatgtttgga gggcacggtt agcagctacg
     8821 tctggatcag agggaaagta acgttcgcac aggcggtcgg cgcagagaga cttgtaagga
     8881 agtggggcgc ctcgaaggaa gaggctgaaa ctgcgattgg aagcggtttg gctcggaccc
     8941 tgaacgccga tcctggcaga gtttctaaga tcagaaagtc atttcttggc taggcgagcg
     9001 accataagca tcgtcgagct ggcttgatgc ttagaacatc tatgaagcgg ctcgttttcc
     9061 tgcttgctat cggcctcgtc ctgcttgccg cgggcatagc cactccccag tccgcaccca
     9121 agaaagcgga aagagatcca cacactgcgc gggtcggcga cttgcaagtc acggtaacgg
     9181 cggctggagc agtttcatcg aatctttttc agccgtaccg aatcccaatc cgtcccgggt
     9241 accatttcgt agttgtgaag gtaaaattca aaaatctagg ttcgtaccct aattgttatt
     9301 caccgagccc gtggcttcgc gtcgatcttg ggttcaacta tagcggcacc acctatccag
     9361 gacctttcaa agggccggaa acttatgatt tgcagccctc agacgaatct gggggtttct
     9421 atgtcttcca ggccaaagac ggaacccatc ccgtcgcgct aaaactcgta cgtagcacgt
     9481 tgaacgagct tggctgcagg aactggccgc accagcacgg ggagaagcca ccccccgacg
     9541 agctcacctt ttccttgcgc aaccttcctg agccggcaaa ctaggccagc accgtatcac
     9601 ctaaaacctc ccccaccctg tgttagcata ttcattcgcc cactcatgga cttttccctg
     9661 accgacgacc agcgcctctt gaaagaaacc atccgccact tcatggaggc ggaaattcgc
     9721 cccatcctgc gcgagtacga gcgcgaggag cgctttcccc tcgagcagat tcgcaagctc
     9781 ggcgagctgg gctgctgcgg catcatggtc cccgaggaat tgggcggctc cggttccgac
     9841 gcggtcacct acgtggtcat gctcgaagag gtcgcgcgcg tctgcgcctc caccgccgtc
     9901 accctcggcg tcaccaattc cgccgtctgc tcgccgcttt ctcgccatgg caccactgcg
     9961 cagaaggaaa aatatctccg ccgcctcgcc agtggtgaaa ttctcggctc cttctgcctc
    10021 accgagcccg gcgccggctc cgacgccgcg gccatcacca cgcgcgccgt ccgcaccgcg
    10081 ggcggctacc gcctcaccgg caccaagacc tgggtctcca ccggcggcgt ctccggcatc
    10141 tacctggttt tcgccaagac cgaccccgcc gcaggcgccc gcggcgtctc cgcatttctc
    10201 gtcgagcccg agtttcgcgg cttccgcgtg agccgctacg aggataagat gggactgcgc
    10261 atgtcgcgca cggcggaaat caccttcgac gagtgcgagg ttcccgacga aaatcgcctc
    10321 ggcgaagaag ggcaggggct caaggtggcc ttggaaacgc tcgatggcgg gcgcatcggc
    10381 atcgccgcgc aagccgtcgg cgtggcccag ggcgcgctcg aagcttcggt gcgctacgcc
    10441 aagcagcgcc gcgcgttcgg caaaaccatc ggcgactttc aggccatcca atggatgctg
    10501 gcggatatgc agacggaaat tgaagcggcg cgcggcctca cctattacgc cgcgtggcgc
    10561 tacgatcagg atgccaggcg cgcgtcctcg gcgggcccct ccgcgggtcc cttcaaggaa
    10621 aagacgcgcg ccggcgccga tgctgcgcgc gccaaacttt attccagcga aatggtgaac
    10681 cgcgtggtct acaaggccgt gcagatccac ggcagcatgg gctattcccg cgagaccgac
    10741 gtcgagcgca tgtaccgcga tgcgcgcgtc atcaccatct atgaaggcac tagcgaaatt
    10801 cagcgcatgg tcatcgcccg cgatctgctc tcgcgcggcg ccgtctcttc gaatgattct
    10861 cggcagggcg cggcttcagc cgtgccgcga ggcgccgctg aatgattgcc acctccgccc
    10921 cccgcaagcg cgtcgtcagc ggcatgcgcc ccaccggccg cctgcacatt ggccactatt
    10981 tcggcgccct cgccaactgg atcaagctgc aaaacgatcc cgcctacgaa tgtttctact
    11041 tcatcgccga ctggcacgcg ctcacgtccg attacgccga cacttcgcag gtctcgcaaa
    11101 acaccatcga aatcgccacc gactggctcg cctccggcct cgaccccgcg aagtccacat
    11161 tcttcgtcca gtcccaggtc cccgaacacg ccgagctgca tctactcctc tccatggtca
    11221 cgcctctcgg ccgtctctac agcttgccca gttacaagga acagcttgaa aatattaaag
    11281 ataaaaacct ccaaacctac ggtttcttgg ggtatcccgt tctgcagtgc gcggacattg
    11341 tgatttacgg cgagccgaaa gctcctttgc tggtgcctgt gggcgaggat caggtttcgc
    11401 atgttgaact ttctcgcgaa attgtgaggc gagtgaatat tttctatggt cttgatgtgg
    11461 acccgaagct tttccttcga gagaacgagc acgctttgcg acaaatcgcg agtcactcca
    11521 atgctcattt accttcagat tggaaatatc cggtcgagcc gggtgtgcag acgtttctga
    11581 ttggaagatt ccgttcagcc gctgccgagg agggcatcga caattttctt aaagtagtag
    11641 ggaatcttgg gaatttcgtc cggtgggacg aagtcttgaa ggaacccggg gtgatgctaa
    11701 cgaagacgcc ccgcatcccc ggcaccgacg ggcgcaagat gtcgaag
//
LOCUS       DASYVO010000113        15007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_130865, whole genome shotgun sequence.
ACCESSION   DASYVO010000113 DASYVO010000000
VERSION     DASYVO010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 15007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15007
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130865"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VGR03_03725"
     CDS             complement(<1..693)
                     /locus_tag="VGR03_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1993421.1"
                     /translation="MLPGLRLRQARERLGLTYRDVERASYELAARRGRPDFIVHISRL
                     ADIENRGVVPGLHKLYTLAAIYRLNPLELFRWYDVPIEECFHDGTSFPVPQTHLMAAP
                     TSLRIPMKFDPAFDPRRTEFLSRMVEKWGHFEGVLTNGHNHHIYGYIGMSDRRMMPLL
                     RPGSIVLVDASVHKIDDGNWTTEHDRPMYFVEVRDGYRCGWFHQEGSRLVMQPHPLSH
                     CLPETWRVPEEAV"
     gene            complement(2384..3730)
                     /locus_tag="VGR03_03730"
     CDS             complement(2384..3730)
                     /locus_tag="VGR03_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEV1993422.1"
                     /translation="MAATADRFSRLAALKPTRPMPARPAAIRTPGEEDTLGRLLGAGV
                     AKNHFGEHLAIRNWFSTSEFTDPSSTTLELLSRTRDESLSRKTRAALQDPEKWLFLDT
                     ETTGLAGGTGTYAFLIGLAWWDAGGLQVEQFFMRDFAEEHSLLQELSQRVAERPVLVT
                     FNGKSFDWPLLENRFTMTRSIAVPKLAAHLDLLHPARALWKLRLGSVRLVELERHVLD
                     APRLGWHRENDVASALIPQYYFDYLRGGPAEPLAGVVRHNQMDLRGLAALFCKINALL
                     SEAPGAANKIESLDLFGLSRFLQRRGDADRAHSACAQALEIGLPAEFRPKARRELALM
                     AKRRGEHARAAEIWLEIVADSHDGIHACEQLAIYYERHAKDLAKAAQFAKLAIATLRR
                     QRAISRDPFLTARFARLENKFLQRVARLEKKSRPPSPAQPQGARVVAAPKASSGLW"
     gene            complement(3731..6301)
                     /locus_tag="VGR03_03735"
     CDS             complement(3731..6301)
                     /locus_tag="VGR03_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1993423.1"
                     /translation="MPESLAVRRAAMPTAITRVHEVLDAFAARDFNGEILTAVRHFPA
                     REAQWADFPTWVHPDLASAYAAKGIRRLYTHQASAAEAVHAGKNVVIVTPTASGKTLC
                     YNLPVLNAVLENSDSRALYLFPTKALAQDQLAELHDINQRLENRFGVFTYDGDTPADA
                     RRSIREKGHIVLTNPDMLHTGILPHHTRWTRLFENLRYIVLDELHTYRGVFGSHLCNV
                     LRRLRRIARFYDRDPQFICCSATIANPGDLASRLLESEVEVLNANGAPAAEKTFAFYN
                     PPVVNRALGIRRSYINESSRVAQEFLKRDLQTMVFANSRLHTEILLTYLQQANPQLPG
                     KPETIRGYRGGYLPNERREIERGLRDGKIRGVVSTSALELGIDVGSLDTVVMAGYPGT
                     IAATWQRAGRAGRRSGSACAVLVASSAPLDQFIVRHPDYFFGNTPEHAFIQPDNLEIL
                     INHLKCAAFELPIAPGDKFGEVDLPDLCARLAEAGYLHLAGKNYHWTQEAYPADTISL
                     RSVTSDNFVIVDVTGAPTVIGEVDFPSALVFLHEKAIYIHGGQQFHVEHLDFKERKAY
                     VKRVDVDYYTDAVRYTQVRVLEIATTAFCAATTSVTDAASFVGAQHAAPLRARSLLPD
                     KSVILSDAKDLNLTTASNPSAHSHGDVLVRSQVVGFKKIKFFTNENIGAGKLELPENE
                     MHTTSYWITLERPLLESLPFTVSERQSGMFGLLHALESVATLLLMCDRRDLGTAIGER
                     PPFADSESTSPLVGAQHAAPHPDTIDNFTPSRLGDALTANAKEFFEPNLYLFDAYPGG
                     IGFSEPLFRTHALLVQKTRELISACPCEQGCPSCVGPAGDLAPRAKEAALAILDRLCA
                     "
     gene            complement(6450..7649)
                     /locus_tag="VGR03_03740"
     CDS             complement(6450..7649)
                     /locus_tag="VGR03_03740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1993424.1"
                     /translation="MPESAEKQSWLNRTVLGVGLTSLFSDWSHETATAVLPAFLAAIG
                     AGPVWLGAIEGIADGLSSFTKLASGHFTDRLKHRKPLAVFGYAFTALATASFAFATHA
                     YHVLFARSAAWLGRSVRSPAKKALLAADVPPNAYGRAFGFERLMDTVGAIAGPLTALW
                     LLEVTGHSYRKVFLWTLLPGLVAVAVFWLLVRERPIAARPQRSFFLGLKALPLPFRRL
                     LVGVGVFGAGDFSHTMLILYAARTLTPGHGLARAASIAVGLYTLHNVFYAGSAYLSGW
                     LSDHVPSRKAVLAAGYALAGVTALLLCTGTNSLWLLALIFVLAGLYVGTEEALEDSLS
                     AELVPKEQHGMAFGTLAAVNAVGDLVSSLLVGFLWSAFNVQTAFTASAILFFVGALLI
                     LRLRTHA"
     gene            7887..8636
                     /locus_tag="VGR03_03745"
     CDS             7887..8636
                     /locus_tag="VGR03_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993425.1"
                     /translation="MNFRRLFTSSSVICMLLGVFAFYYARPITILCYAKWKARNAPEM
                     WVVPKPLSDITLSAANGRKFSYFGYEFEVPWTEVKQERKLQSIAIVNFSNGAVVTILN
                     PVGELGELQILQKEAAKRGRDVKAVFGEDASRSNYSLRSKILNLTPRDLRFFSSRQEM
                     VSDSILLILKSIWTKRIKGGLYSFQTEWLHGFQEGGPAEDKMVIIEAWDANDRKMEVW
                     IGSEPRAPKFSQAEVNRILFSLRPISPSPPQ"
     gene            complement(8672..9019)
                     /locus_tag="VGR03_03750"
     CDS             complement(8672..9019)
                     /locus_tag="VGR03_03750"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEV1993426.1"
                     /translation="MTHEKFEGERTLMRIHIGESDKWHGKPLHEAIVGMLRKDGFSGA
                     TVLRGVGGNGGSSIYHTDKLLRLSQDLPIILEVIESIERIEKILPRLDEMVDGGLITL
                     EKVRVILYRAAKK"
     gene            complement(9158..9568)
                     /gene="crcB"
                     /locus_tag="VGR03_03755"
     CDS             complement(9158..9568)
                     /gene="crcB"
                     /locus_tag="VGR03_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1993427.1"
                     /translation="MRARCGEQQIGATLRLALIAIFGAIGTLARYGLQGVVQVRTAST
                     FPLGTLLVNLSGCFLLGLIGQFTLKHMVISPDWRMAIAIGFFGGYTTFSSFGWETAKM
                     LEAGEWAWARTYVGASVVAGLLLSVAGIRLANKF"
     regulatory      complement(9610..9682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(10140..10934)
                     /locus_tag="VGR03_03760"
     CDS             complement(10140..10934)
                     /locus_tag="VGR03_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993428.1"
                     /translation="MPTGKLDGVNSHSWRLKIRWANKHIENLKASLDAVESHPYPVTV
                     KRDPKTRERVYYATKVPDISSEFALSAGDILHNLRSALDHLACGLVRANGGTITNSTE
                     FPIIEYAPRTDRDKARLAGKIEGIRQEAKDYILSIQPYRQGNAASALGRLHKLNIRDK
                     HRLLLTFATAMTKFNIGQHWRATRVGTGQGYIPDHWVMSMVPLLEEGQKLLVDPPNAK
                     VNENPDVRLQIMVNEIGVCEGEPLIQVLKTSLYWVQGITADCERFL"
     gene            complement(11030..13261)
                     /locus_tag="VGR03_03765"
     CDS             complement(11030..13261)
                     /locus_tag="VGR03_03765"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV1993429.1"
                     /translation="MKRAFLFVVFLFAVLPAFATVFGTVRGIVHDPQHRPVPDVTVTL
                     KAKSSSYVQTAQTDANGEFHFDAVPLGEYSVTASAASFASEEQAIGVLSGTAPILHFE
                     LKLGSQAESITVSADAGSGQLESITPTTLVNRQEISDTPGASRTNSLALITDYVPGSY
                     VVHDQLHIRGGHQVTWLIDGVAIPNTNIASNLGPQLDPKDIDTMEAQRGSYSADYGDR
                     TYGIFNVAPRTGFERDNDAELVLSAGNFYQTDDQLNFGGHTNRFAYYASVNGNRTNLG
                     LQTPTSAVIHDAANGFGGFTSLIYNAGSHDQFRLVAQLRRDFYQVPFDPNDPGTQGQF
                     LRDASRENDSFVTFSWVRTFSPGLMLTVSPFFHHNSANYQSSLQDLPSSATENRRSKY
                     EGGQATLSWVKNRSNFRAGLYGFAQQDDQLFGLLCHDHSQCQNVDPPERHSPGGGLAA
                     VYAEEQFKATSWLSLNAGLRQTHFSGGVVENATSPRIGASLRIAKLHWVFRAFYGHFY
                     QAPPLLTVSGPLLEAASNQNLNFIPLRGERDEEHQFGVTIPFHGWTLDASNFLTRAEN
                     FFDHNNFNNSDLFFPVTIQGARINGWELTLRSPRIRDRAQVYLTYSNQLALAFGNING
                     GLTDFSFGDGFKLLDHDQRHTLHFGARYDLPFHSYAATDVYYASGFTNGDLMLPGDHL
                     QPHTTFDLSLGKSFGDKFFVNIQALNVANRRVLLDNSFTFGGTHYLNPREIFVQLRYR
                     FHY"
     gene            complement(13352..13717)
                     /locus_tag="VGR03_03770"
     CDS             complement(13352..13717)
                     /locus_tag="VGR03_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993430.1"
                     /translation="MLAVVLLYAPLAGAAWSSHQSACCMSDQCPIPAHHHQKTPAAPT
                     NHMDCGHDTTRMMACSMSCCQDSDRLLAASIAFVLPPSVAVAAAAAIESPVELAKLLD
                     FPRSIEPLSPPPRFGSAAV"
     gene            13983..14444
                     /locus_tag="VGR03_03775"
     CDS             13983..14444
                     /locus_tag="VGR03_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993431.1"
                     /translation="MAHSYIRFDFGTDEEKAQHARHKLDGWRQAFRLDKKLQYKLDRP
                     ENTAAAASPTTAIADPVESTGKSKAKAAEKPKGKASTASSEPSAKKPAAVNGKVSLLV
                     RLYFSSHEKLSEQRWLDRIPSEEPFKGASPKVVHHSDPEFERTDKQFEALE"
     gene            14616..>15007
                     /locus_tag="VGR03_03780"
     CDS             14616..>15007
                     /locus_tag="VGR03_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV1993432.1"
                     /translation="MALQTRVKFAKLDAPKVHGLEHDTLIRIYRTMFLSRRLDDREIQ
                     LKRQNKIYFQISSAGHEAVCAAMGLFLRPGVDWIYPYYRDRALCLMLGVTPLETLLAA
                     VGAKDDPSSGGRQMPSHWGQPKLNIVSKS"
BASE COUNT         3247 a         3853 c         4785 g         3122 t
ORIGIN      
        1 taccgcctct tccggcacgc gccacgtctc cggtaaacaa tgcgaaagcg gatggggctg
       61 catcaccagc cgcgaacctt cctgatggaa ccacccgcaa cgatatccat cacgcacttc
      121 cacgaagtac atcggccggt catgctcggt cgtccaattg ccatcatcga tcttgtgtac
      181 ggacgcatcc accagcacga tgctcccagg acgcagcaac ggcatcatgc gccggtcgga
      241 catgccaata tacccataga tatgatggtt atggccgttc gtgagcacac cctcaaagtg
      301 cccccacttt tccaccatgc gcgacaggaa ttccgttcgc cgcggatcga acgccgggtc
      361 aaatttcatc ggtatccgca gcgacgtcgg cgccgccatc agatgcgtct gcggcaccgg
      421 gaacgacgtt ccatcatgga aacattcctc gatgggaacg tcgtaccagc gaaaaagttc
      481 cagcgggttc aaccgataga tggccgccag cgtatacaat ttgtgcagac ccggcacgac
      541 gccacggttt tcaatatccg ctagccggct gatgtggaca atgaaatcgg gacgccctcg
      601 gcgtgcggcc aattcgtagc tggcgcgctc gacatctcga taggttagtc cgagtcgttc
      661 gcgggcctga cgcagacgaa ggccgggcag catcaattcc tctgaagttg tgtggatccc
      721 cacaagcaga gtacaacaga attaccacac tgttaagttt tcggaacaaa gattcatcgc
      781 aatccacatg cctgttgaaa aagtattgca gatttagagg gcgcaccttc agtcgcggca
      841 taagtcctgc gttttcttca ggtgtacctg ccgatgaagc gccccgcgaa ccgcctcgtt
      901 ggttggaact ttggccgcgg ttaccgccct gagcctcata gacttgaggt cgtgtccccg
      961 ttaacccttc tttccgtttt tgtggcacag ccattcctgg ctgttctggc ggctcgttac
     1021 tcgtcgctcg ccacttcctg tgcctttcag acagccccaa ttggattctg ctattctgaa
     1081 aacgatatgg cctctcactt gaaacgcact tttatcgggc tgcgcgctct cttcttcagc
     1141 ctcactctct cccttggtgg tgtcgtcgcc cccgcacagg aagctgtcgt cgctccacct
     1201 tccgcgtatt ccaagcccgc caatgacgcc gatttcattg ctgccgccga cgaagtcctc
     1261 ggccagatga gcacgatcac cgggctgaag ctgcgcacac cgctgaaaaa atccctgcgt
     1321 tcgcgcgagg aaatccgcgc tttcgtcatc aagcagatga acgaagaaaa gaatcccgcc
     1381 gagcgctatg ccgaccagcg tagcgcggaa gctttcggcc tccttcccaa aggattcgac
     1441 ctcgattcgt tcatggtgaa cgtgctcacc gaacaggtcg agggacttta cgatccgaag
     1501 acgcgcgaat tttacatcgc ggactggagc ccgcttgccg accagcgcat ggtcatggcc
     1561 catgaattga cgcacgcgct cgaggaccag catttccaga tcgaggcgtg ggtgaaagca
     1621 gcccgtccca atgaggatgc cgagctggcg cgcgacgccg tactcgaggg cagcgccatg
     1681 gccgccatgg tcgattacct catgcttggg acggggcgct ccctgaagga tttgccggat
     1741 ttcgatccgg ggatgttgat tggcgatctg ggcagcactc cgacgctgca aaaagcgccg
     1801 cctttcctca aggacgcgct gatcttccct tatataggcg gactgaactt ctcagctgcc
     1861 gtcatgaaaa ataccggctg ggccgccctt tcgggcgtgt tcgagaagcc gccggtttcc
     1921 acacaacaga tcctccatcc ggcgctctat cggtccggaa agactcctgc aagtgtcgct
     1981 cttccgcggc tcgagaaagt gctcggtgac aactggtcaa agctcgacga aaacatcatg
     2041 ggtgaatttg gctggaagga agtcttgaag caattcctgg acgacgaccg ggccaagagg
     2101 ctggccgcgg cctgggatgg cgaccgctat attctctacg agcacaatca cacgaaaaag
     2161 ctcgtcctgg tgacccgcct gcacctggat agcgaagaac acgcggcacg cttcttcgga
     2221 caatattcgg aagcgctgga gaagaaatac tcggagcgcg cgaatttgct tcgccgcccg
     2281 aacttttttt catttgatac tcccgatggc ggcgtgttcc tggattgttt gggagacgag
     2341 tgcgtgacca tggaaggcgc cacgcgcaat attttcgacg ctttcaccaa agccctgagc
     2401 tggcctttgg cgccgcaacc acccgagcac cctgcggctg tgccggagaa ggcggccgtg
     2461 attttttctc cagccgcgcg acccgctgca gaaacttatt ctctaggcgc gcaaaccgcg
     2521 ccgtgagaaa cgggtcgcgt gagatggcgc gctggcgccg cagcgtggca atcgccagtt
     2581 tggcaaattg cgccgctttg gccaggtctt tggcgtgacg ttcgtagtaa atggccagtt
     2641 gttcgcaggc atgaatgccg tcgtgcgaat cggcaacgat ttccagccag atctcggcgg
     2701 cgcgcgcgtg ttcgccgcgg cgcttggcca tcagcgcaag ctcgcggcgc gccttagggc
     2761 ggaattcggc gggcaatcca atttcaagcg cctgcgcgca cgcggaatgc gcgcgatcgg
     2821 cgtctccccg gcgttggaga aatcgcgata gcccgaacag gtcgaggctc tcgattttgt
     2881 tcgcggcgcc gggcgcttca gaaagcagcg cgttgatctt gcagaagaga gcggcgagcc
     2941 cgcgcaaatc catctgattg tggcgaacga cgccggccag cggctcggcg ggcccgccgc
     3001 gcaaataatc aaagtaatac tgcgggataa gcgccgacgc cacatcgttc tcgcgatgcc
     3061 agcctagccg gggcgcgtcg aggacgtggc gttcgagttc cacgagccgc accgaaccga
     3121 gccggagctt ccacaacgcg cgcgcgggat gcagcaaatc aagatgcgcc gccagtttcg
     3181 gtacggcgat ggagcgcgtc atggtgaaac gattttcgag caacggccaa tcgaaggatt
     3241 tcccattgaa ggtaacgagc accggccgct cggcgacacg ctgagacaat tcctgtagta
     3301 gcgaatgctc ttccgcaaag tcgcgcataa aaaattgctc gacctgcaat ccgcccgcat
     3361 cccaccacgc caggccaata agaaacgcgt acgtgccggt gccgccagcg aggccggtgg
     3421 tttccgtatc gagaaaaagc catttttctg gatcttgcag tgcggcgcga gttttgcggg
     3481 agagcgattc gtcgcgcgtg cgggaaagca attcgagtgt tgtggaagag gggtccgtaa
     3541 attccgaagt ggagaaccag tttcgaatgg ccaggtgttc gccgaaatga tttttagcga
     3601 cgcccgcgcc gaggagccgg ccgagggtgt cttcttcgcc gggcgttcga atcgcggcag
     3661 ggcgcgcggg catgggccgc gtgggcttca gcgcggcaag acgcgaaaat ctgtcggcgg
     3721 ttgcggccat ctaggcgcac agcctgtcga gaatggcgag ggcggcttct tttgcgcggg
     3781 gggcgagatc gccggcgggg cctacgcagg aggggcagcc ttgttcgcag gggcacgcgg
     3841 agatgagttc gcgcgttttt tgtacgagga gggcgtgcgt tcgaaagagg ggttcggaga
     3901 agcctatgcc gccgggatag gcgtcgaaga ggtacaaatt tggttcgaag aattctttgg
     3961 cgttggcggt gagtgcgtct cctaggcggg acggagtgaa attgtcaatc gtgtcgggat
     4021 ggggcgcagc gtgctgcgcc cctacaagag gggatgtaga ttcggaatca gcgaacgggg
     4081 gacgctcgcc gatggcggtg ccgaggtcgc ggcggtcgca catgaggaga agggtggcga
     4141 cggattcgag ggcgtggagc aggccgaaca tgccgctttg gcgctcgctc acggtgaagg
     4201 gaagtgattc gagcagcggg cgttcgaggg tgatccagta ggaggtggtg tgcatttcgt
     4261 tttcggggag ttcgagtttg ccggcgccga tattctcgtt ggtgaagaat ttgattttct
     4321 tgaagccgac gacttgggag cggacaagga cgtcgccgtg ggagtgcgcg gatgggttgg
     4381 aggcggtggt taggttgaga tccttcgcgt cgctcaggat gacagattta tcgggcagta
     4441 gtgatcttgc ccgtaggggc gcagcatgct gcgcccctac aaaggaagcg gcgtcggtga
     4501 cagaagtggt ggcggcgcag aatgcggtgg tggcgatttc gaggacgcgg acctgggtgt
     4561 agcggacggc gtcggtgtag tagtcgacgt cgacgcgctt gacgtaggcc ttgcgttcct
     4621 tgaagtcgag atgttcgacg tgaaattgct ggccgccgtg aatgtagatg gctttttcgt
     4681 gcaggaacac cagagcgctg ggaaaatcca cttcgccgat gacggtgggc gcgccggtaa
     4741 cgtcgacgat cacaaaattg tcgctggtca cggagcggag actaatggta tcggcgggat
     4801 aggcctcctg cgtccagtga taattcttgc cggctaagtg cagataacct gcttcggcga
     4861 gtcttgcgca caaatcgggc aggtcgacct cgccgaactt atcgcccgga gcgatgggca
     4921 attcgaacgc cgcgcatttc aggtggttga tgaggatttc gagattgtcc ggctggataa
     4981 aagcgtgctc gggagtgttg ccgaagaaat agtccgggtg gcgaacgatg aattgatcga
     5041 gaggcgccga agaagccacc agcacggcgc aagcgcttcc gctgcggcgg ccagcgcggc
     5101 cagcacgttg ccaagtggcg gcgatggtgc cggggtatcc ggccatgaca acggtatcga
     5161 gggagccgac atcgatgccg agttcgagcg cgctcgtgga aacaacgcca cggatttttc
     5221 catcgcgcag gccgcgctcg atttcgcggc gctcgttcgg caggtagccg ccgcgatagc
     5281 cgcgaatggt ctccggtttt cccggcaact gcggatttgc ttgctgcaga taggtgagaa
     5341 gaatttccgt gtgcagccgc gaattggcga agaccatggt ttgcagatcg cgtttcagga
     5401 attcctgggc cacgcgagag gattcattga tatagctgcg gcggatgccg agggcacgat
     5461 tcacgacggg cgggttgtaa aaggcaaacg ttttttccgc agctggtgcg ccgttggcgt
     5521 tgaggacttc gacttcggat tcgagaaggc gtgaagcgag gtcgccggga ttggcgatgg
     5581 ttgcggagca gcagatgaat tggggatcgc ggtcgtagaa acgggcgatg cggcggaggc
     5641 ggcggaggac gttgcagagg tggctgccga agacgccgcg gtaggtgtgc agttcgtcga
     5701 ggacgatgta gcgcaaattt tcgaacaggc gcgtccagcg cgtgtgatgc ggcagaattc
     5761 cggtgtgcaa catgtctgga ttggtcagga cgatgtggcc tttttcgcga atggatctgc
     5821 gcgcgtcagc gggagtgtcg ccgtcgtaag tgaaaacgcc aaagcggttt tcgagacgct
     5881 gattaatatc gtgaagttct gcgagttggt cttgtgcgag tgctttggtg gggaagagat
     5941 acagcgcgcg gctgtcggaa ttttcgagga ccgcgttcag caccggcagg ttgtagcaga
     6001 gcgtttttcc agaggcggtg ggagtgacga tgacgacatt cttgccggca tggaccgctt
     6061 ctgctgcgga tgcttgatgg gtgtaaaggc ggcgaatgcc cttggcggcg taggcggagg
     6121 cgaggtctgg atgcacccaa gtagggaaat ccgcccactg ggcctcgcgc gcgggaaaat
     6181 ggcgcacggc cgtgaggatt tcaccgttga agtcgcgggc ggcaaaggcg tcgagaactt
     6241 cgtggacgcg ggtgatggct gtcggcatgg cggcgcggcg aactgctagc gattcgggca
     6301 tgaatggccc ctttccttaa ttcgcctttt attcgctcac ggtagcatga ggcgatttgg
     6361 ggcgcaacac agaaaatgga cggaccccct taacacagag gtcacacagg aaagtcgtga
     6421 gctgcaggct gtcggcgcgc caggccagat caggcgtggg ttcgcagacg taggatgagg
     6481 agagcgccga cgaagaagag gatggcggag gcggtgaagg cggtctggac gttgaaggcg
     6541 ctccagagga agccgacgag gaggctggag acaaggtcgc cgacggcgtt gacggcggcg
     6601 agggtgccga aggccatgcc atgttgctct ttggggacga gctcggcgga gagagaatct
     6661 tcgagagctt cttcggtgcc gacgtagaga ccggccagaa caaaaatcag ggcgagcagc
     6721 cacagggaat ttgttccggt gcagagaagg agtgcggtga ctcctgcgag ggcgtagcct
     6781 gcggcgagca cggctttgcg gctggggacg tggtcgctga gccatccgct gagataagcc
     6841 gagccggcat agaagacgtt gtgcagagta tagaggccga cggcgatgct ggcggcgcgg
     6901 gctaggccgt ggccaggcgt gagcgtccgc gctgcgtaca gaatcagcat ggtgtgggag
     6961 aaatcgccgg cgccgaagac tcctacgccc acgagcaatc ggcggaaggg gagcggcagc
     7021 gccttcaaac caaggaaaaa ggatcgttga gggcgggccg cgatggggcg ctcgcgtacg
     7081 agaagccaga aaaccgcgac ggcgacgagg ccgggcagaa gcgtccagag aaaaaccttg
     7141 cggtacgaat ggccggtgac ttcgaggagc cagagcgcgg tgagtggtcc cgcgatcgcg
     7201 cctaccgtgt ccatcaagcg ctcgaaaccg aaggcgcggc cgtaggcgtt cggtggcacg
     7261 tcggcggcga gcagggcttt cttcgcgggt gagcggacac tgcgtcccag ccaagctgcg
     7321 gagcgggcga aaagcacatg atacgcgtgg gttgcgaagg cgaacgacgc cgtcgcaagg
     7381 gcggtgaagg cgtagccgaa gacggcgagg ggcttccggt gtttcaagcg atcggtgaag
     7441 tggcctgagg caagctttgt gaagctggag agaccgtcgg cgatgccttc gatggcgccg
     7501 agccagactg ggcctgcgcc gatggcggcc aggaatgcgg ggagcaccgc tgtggcggtt
     7561 tcgtggctcc agtcgctgaa gaggctggtg aggccgacgc ctaggacggt gcgattgagc
     7621 caggattgtt tttctgcgct ctcgggcata ggaaagcagt ttacagtcaa cagttcgcag
     7681 ttaacagttt tgagaaaaac cataaagcgg cagtcttgaa agtgtggcat ttcgagggcc
     7741 taaaagagtc gatggtgatt tagcaaaata tggttaggaa ccagaagagc atcattttcg
     7801 gtaaacaggt ttactggata gcaagccgtg tacaatgcct ctgttcggaa tctcaaacag
     7861 aaaattctag ctggagagtt tcgcgcatga attttcgtcg cctcttcacg agttcgtcgg
     7921 tcatctgcat gcttctgggt gtattcgcgt tctactatgc tcgcccgatt acgatattgt
     7981 gctacgcaaa gtggaaggcc cggaatgcgc ctgaaatgtg ggttgtgcca aagccgctat
     8041 cggacatcac tctctcggcg gccaatggac ggaagttttc gtattttggg tacgaattcg
     8101 aggtgccatg gacggaagtg aagcaggaga ggaagctgca atccatagca atcgtaaatt
     8161 tttcaaatgg ggctgtcgtt acgattctta atccggtagg tgaactcggc gaactgcaga
     8221 tactccagaa ggaagccgcc aagcgtggaa gggatgtcaa ggctgttttc ggcgaagatg
     8281 cttcgcgctc gaactattcg ctgcgttcca agatcctgaa tttgacacct agagacttac
     8341 gttttttttc gtccagacag gaaatggtca gtgattcgat tttgctcatc ctgaaaagta
     8401 tatggacaaa aagaataaag ggcggtttgt attcctttca gaccgaatgg cttcatggtt
     8461 tccaggaggg cggtccagca gaagacaaga tggtcataat cgaggcctgg gacgcaaatg
     8521 atcggaaaat ggaggtttgg atagggtcag agcctcgtgc gcctaaattt tcccaagctg
     8581 aggttaatcg catacttttt tcccttcgcc caatttctcc gtctccacct caatagctcc
     8641 cgcaccctaa tctgtcggaa ggagtgcgga attatttttt ggcggcgcgg tagaggatta
     8701 cgcgcacctt ctcgagggtg atgaggccgc cgtcgaccat ttcgtcgagg cgggggagga
     8761 ttttttcgat gcgctcgatg gattcgatga cttcgaggat tatcggcagg tcctgcgaca
     8821 agcgcagcag cttgtcggtg tgatagatgc tggagccgcc gttaccgccg acgcctcgca
     8881 gcacggttgc gccagagaag ccgtctttgc gcagcatccc gacgatggct tcgtggaggg
     8941 gtttgccgtg ccatttgtcg gattcgccga tgtggatgcg catcagagtg cgttcgcctt
     9001 cgaatttttc gtgggtcatg ggtgaacctc gtgactggtg actagcgact cgtgttccgt
     9061 gaatcgtgat ccgtgactcc tgacgttgag atccttcggg ccgacccgcc cggcctcagg
     9121 atgacacatc gcgagagtgc ttcaacatgt cccgtcatca aaacttgttg gcgaggcgaa
     9181 ttccggcaac cgacaagagc agaccggcga caacgctggc tcccacatag gtcctggccc
     9241 aggcccactc gccagcttcc agcatcttgg cggtttccca gccaaaactc gagaaggttg
     9301 tgtaaccgcc gaagaagccg atggcgatgg ccatgcgcca gtcgggcgag atcaccatgt
     9361 gcttcagagt gaattggccg atcaagccga ggaggaagca gccggacaaa ttgaccagca
     9421 aggtgcctaa agggaaggtg ctcgccgtgc ggacttgcac gacgccctgg aggccgtagc
     9481 gcgccagcgt gccaatggcg ccgaagatgg cgatcaacgc taaacgcaat gtcgctccaa
     9541 tctgctgctc gccacagcga gcgcgcaaat ggcctgcggc gagggaaaca aaaaactccc
     9601 ggagccgtca ggcagccagg agtcatcagc tccggcgaag aacgccggga cggttatggc
     9661 gaactccatc gccattcgag atttcggctc agtttagcat catccggcac tgccggatgg
     9721 gcaaaaccac ggttgtgccc gttagtggca aaggagcaaa ggctcctggg aagtctgcag
     9781 aggcggaaga ttcctggtta accataacac caccctcccc cctgtttttt gtaagtgttg
     9841 catctaaagg gcttagccgg gcagtaagtc ttttgtttgc aacacttgcg gggagatcca
     9901 taagtgttga ttctaaaggg acttaggctg caccaaaatt gtgcaactct ctcgtttgtt
     9961 tgcagtgggg gatgggcgct ggaccgaggc taccggatta acgacagatg tacctaatgc
    10021 ttagactacc agagaaaggg tagtctgtca agagaaagga acaacagcgg ggagcaagtc
    10081 aaaacccgca gcctttaaga cgaaggctgc ggcactccgc tcgtccaaaa attacaactt
    10141 cacaggaatc tctcacaatc tgccgtaata ccttgcaccc agtagagaga ggtcttcaaa
    10201 acttggataa ggggttctcc ttcgcacacc ccgatttcat tcaccattat ttgaagtcga
    10261 acatcgggat tctcgttgac tttcgcatta gggggatcaa caaggagttt ttggccctct
    10321 tcaagaagag gaaccatgct cataacccag tgatcaggaa tgtatccttg gcccgtgcca
    10381 actcttgtag ctctccagtg ttgtccaata ttgaattttg tcatcgccgt tgcaaatgtc
    10441 agcaacagcc gatgcttgtc tctaatgttg agtttatgga gacgcccaag ggcgctcgcc
    10501 gcattccctt gtcgatatgg ttggatactg aggatgtagt ccttggcttc ttgccgtatt
    10561 ccctctatct ttccagcaag tctggcctta tctctatcgg ttctaggagc atattcgata
    10621 atgggaaatt ctgtgctgtt cgtgattgtg cctccgttgg cccgaacaag accacacgcc
    10681 aagtgatcca aggcacttct gagattatga aggatatccc cagcactcaa tgcgaattcg
    10741 ctggaaatgt caggtacttt cgtcgcgtag tagacgcgct ctctcgtctt agggtcgcgc
    10801 tttactgtaa ccggataggg atgcgactcg acggcatcca aagaggcttt caagttttcg
    10861 atgtgcttgt tggcccatcg gatttttaga cgccatgagt gggaattcac gccatcgagt
    10921 ttacccgtag gcatattagt tagctaagta tattcttgct cttggaagtg aatcaggcgg
    10981 aacaggtttt ttgtttcttg aacaagctgc acgcggatcc tcaggaaatt caatagtgga
    11041 aacggtagcg cagttggaca aagatttctc gcggatttaa gtagtgagtt ccgccgaagg
    11101 taaaactgtt gtcgagcagg acgcggcgat tggcgacgtt cagcgcttga atgtttacaa
    11161 aaaacttgtc accgaaactt tttcccagcg agaggtcaaa ggtggtgtgc ggttggagat
    11221 gatcgccggg tagcatgagg tcgccattgg tgaagccgga ggcgtagtag acgtcggtcg
    11281 cggcgtagga gtggaatggg agatcgtagc gcgcgccgaa gtgcagggtg tggcgctgat
    11341 catgatcgag gagtttgaag ccgtcaccaa aggagaagtc ggtgaggcca ccgttgatat
    11401 tgccgaaggc gagggcgagc tgattcgaat aggttagata aacctgagcg cggtcacgaa
    11461 tgcggggtga gcgaagcgtg agctcccagc cgttgatgcg cgcgccctga atggtgacgg
    11521 gaaaaaacag gtcggagttg ttgaaattat tgtggtcgaa gaaattctcg gcgcgcgtga
    11581 ggaaattgct cgcgtcgagg gtccatccgt gaaagggaat ggtgacgcca aactggtgct
    11641 cttcatcgcg ttctccgcgg agcggaataa agttcagatt ctgattgctg gcggcttcga
    11701 ggagcggacc ggaaacggtc agcaaaggcg gcgcctggta gaaatgtccg tagaaggcgc
    11761 ggaaaaccca gtggagtttg gcaattcgta gggaagcgcc gatgcgcggg ctggtggcgt
    11821 tctcgaccac gccacctgag aaatgggttt ggcggaggcc ggcattcaag ctgagccagg
    11881 atgtcgcttt gaactgttct tcggcgtaga ccgctgcgag tccaccacct gggctatgtc
    11941 tctccggcgg gtccacattc tggcactgcg agtgatcgtg gcaaagcaac ccaaaaagtt
    12001 gatcgtcctg ttgagcgaag ccatagaggc cagcgcgaaa attactacgg ttcttgaccc
    12061 acgaaagcgt ggcctgacca ccttcgtact tcgatctgcg gttttcggtt gcggaactgg
    12121 ggaggtcctg caaggagctt tgataattcg cgctgttgtg atgaaaaaag ggcgagacgg
    12181 tgagcatgag tcccggactg aaggtgcgga cccatgagaa agtaacaaaa gaatcgttct
    12241 cgcggctggc gtcgcggagg aactggcctt gcgtgccggg gtcgtttgga tcgaaaggga
    12301 cttgatagaa gtcccgtcgc aactgcgcga cgaggcggaa ctggtcgtga gaaccggcgt
    12361 tgtagatcaa cgaagtgaag ccgccgaatc cgttggccgc gtcgtgaatc actgcggaag
    12421 tgggagtctg taagccgaga ttggtgcggt ttccgttcac gctggcgtaa taggcgaaac
    12481 gattggtgtg gccgccgaag ttgagctggt cgtcagtttg atagaagttt ccggcgctga
    12541 ggacaagctc ggcgtcgttg tcgcgctcga agcccgttcg cggggcgacg ttgaaaatgc
    12601 cgtaagtgcg gtcgccgtag tcggctgagt aactcccgcg ttgtgcttcc atggtgtcga
    12661 tgtcttttgg gtcgagctgt ggaccgagat tgctggcgat gttggtgttg ggaatggcaa
    12721 cgccgtcgat cagccaggtc acttgatggc cgccgcgaat gtgcaactgg tcgtggacga
    12781 cgtaggaacc ggggacgtag tcggtgatca gggccaggct gttggttcgc gaggcgcctg
    12841 gcgtatcact gatttcttgc cggttgacga gggtggtggg cgtgatcgat tcaagctgcc
    12901 ccgatcccgc gtcagccgag acggtgatcg attcggcttg ggagccaagc ttcagctcaa
    12961 agtgcaaaat aggggccgtg ccggagagca caccgatggc ctgttcttcg ctggcgaagg
    13021 aagcggcgga ggctgttacg ctatattcgc caagtggaac cgcatcgaag tgaaattcgc
    13081 cgttggcatc ggtttgtgcg gtttgaacgt agctggagga tttggccttc agggtgacgg
    13141 tgacgtctgg gacgggccgg tgctgcggat cgtgaacgat gccgcgtacg gtaccgaaca
    13201 ccgtggcgaa agctgggaga acggcaaata agaaaactac gaataggaaa gcacgtttca
    13261 tggagcctca ttctcctgaa aaggatcaat cgcgcggcat ccaagccgca gcttgggttg
    13321 atcagcgaat gttggggaag acggcggcgc gtcagacggc cgcggaaccg aatcgagggg
    13381 gaggagagag aggttcgatg gaacgcggaa aatccaagag tttcgctaat tcgaccggcg
    13441 actcgatcgc cgccgccgcg gcgactgcta cggatggagg cagcacgaag gcgatcgaag
    13501 cagccagcaa gcggtcagaa tcctgacagc aagacatgga gcaggccatc attcgggtcg
    13561 tgtcatgacc gcaatccatg tgattcgtcg gtgcggcggg agttttctgg tgatgatgcg
    13621 cggggattgg gcactgatcg gacatgcagc aggcggattg atgcgaggac catgcggcac
    13681 ccgcgagcgg cgcatagagc aacacgaccg cgagcaaaca gatgctcgcg gaaattgaac
    13741 ggcggcgatg ttgacgcgcg gttggcatgt ggtacgcggt ccgatatctg tcgtcagaga
    13801 ctatagcgtt agacgccata gtttgcaagc cgaattataa acagccgctt gcgatgcatt
    13861 ttaatatacg ccagatcctt ctgttttttc gcgcgatgcc ggccgattcg gcggtggcca
    13921 atgcgaactt ttgtggttgg agccatcttg acttagcgtg cggtgcgtcg cagactcgat
    13981 tcatggctca cagctatatt cgatttgatt ttggcacgga cgaggaaaaa gcgcagcatg
    14041 cgcggcacaa gctggatggc tggaggcagg cctttcgcct cgacaagaag ctgcagtaca
    14101 agctggaccg tcccgagaat actgccgcag ccgcttcacc cacgactgca attgccgatc
    14161 cggtggaaag caccggcaag tcgaaggcaa aagcagcgga aaagcccaag ggaaaagcat
    14221 ctactgcatc gagcgagccg tccgcgaaaa agccggctgc ggtcaacggg aaagtgagtt
    14281 tgctggtcag gctctacttt tcttcgcacg aaaagctttc cgaacagcgc tggctggacc
    14341 gcattccgtc cgaggagccg ttcaagggcg cctcgccgaa agtcgtgcat cacagcgatc
    14401 cggaattcga aaggacggac aagcaattcg aggcacttga gtgatttgac cgaggcaaga
    14461 gttatcggga atggggtttc gaggccaaat tggctgcaaa attgagagta cagggtttag
    14521 ccggaaacat caatttggac tgagtccggt ggaaatccgg tataattgaa tcccgcaaag
    14581 gcgcaattcc gaattccaac tcgagggcag aacgaatggc gttacaaacg cgcgtcaaat
    14641 ttgcaaagct tgacgccccc aaggttcacg gcctcgaaca cgataccctg attcgcattt
    14701 accgcacgat gttcctctcg cggcggctcg acgaccgcga gattcagctt aaacgccaga
    14761 acaaaatcta cttccagatc tctagcgcgg ggcacgaagc ggtttgtgcc gcgatgggac
    14821 tgttcctacg cccgggagtc gactggattt atccttacta ccgcgaccgc gcgctttgcc
    14881 tcatgctcgg cgtgacccct ttggaaactc tcttggcggc ggtaggagcg aaagacgatc
    14941 ccagctccgg cggccgtcag atgccgtcgc attggggaca gcccaagttg aacatcgtca
    15001 gcaagtc
//
LOCUS       DASYVO010000114         6139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_102884, whole genome shotgun sequence.
ACCESSION   DASYVO010000114 DASYVO010000000
VERSION     DASYVO010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102884"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..887)
                     /locus_tag="VGR03_03785"
     CDS             complement(33..887)
                     /locus_tag="VGR03_03785"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HEV1993433.1"
                     /translation="MGKFSRSWELVKQSFAILRSDKQLMLFPVLSAVSCFIVTAIIAT
                     GGAFLLLPARAAALAAGERFNPNQSPMFLLGMFTLYVVNYFVIVFFNVALVGVANSRL
                     MGGTWTFRDGIELAWERKGTILQWALVAATVGVILRTMEERLGLIGRLIMRIIGIAWT
                     LACYFVVPVLAFEDLTPIEAVKRSSKLFRDTWGEKVIGGFSLSLVSMVLMLPGIGLLI
                     AATFLASISGMLVGFVLMILYFLLLSVFMSAVGGIFNAALYRYACFKQVPPAFSQELI
                     ASAWAPKS"
     gene            complement(1144..1335)
                     /locus_tag="VGR03_03790"
     CDS             complement(1144..1335)
                     /locus_tag="VGR03_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993434.1"
                     /translation="MTDPRSSSYTDPMEVILSPGLQARLDSIAAQQGRNSASLVHEAV
                     ERLIGYDQRFIRQVEEGLA"
     gene            complement(1489..1890)
                     /locus_tag="VGR03_03795"
     CDS             complement(1489..1890)
                     /locus_tag="VGR03_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993435.1"
                     /translation="MSLGSPLRTFGALALCLLLALPAFPQTGGNIGPSKGEIIAIVAG
                     VAAALTVVGYLIYHETHKHLSITGCVASGADGLTLKSEKDKKVYALSGGSAALKAGDR
                     VTLKGKKSKDPSGKPSFQVETLTKDFGTCNP"
     gene            complement(2136..4190)
                     /locus_tag="VGR03_03800"
     CDS             complement(2136..4190)
                     /locus_tag="VGR03_03800"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV1993436.1"
                     /translation="MRFSKWIVAVIFTFSLCNASYISGQAKSTAKQAVTAHSQPAQKP
                     KAGSPLDNVIDALYAAHTFEQTAISPDGKKLAWVETLVGKDGAPDSNTAIYVADREAS
                     AMPKRITAAASGAPRAEGNVAWSPDSKQIAFLSDAAKPGQLQLYVANPGGPAKKLTNA
                     KGLLAVPKWSPDGKTIAVLYTENATRAAGPLVAETPETGEIQDAFFEQRLALVELTTG
                     GFRQISPADMYVYEYDWSPDGTRFVLTAAHGNGDNNWWVAQLYTLDAGLLGLMKPIYK
                     PSLQIARPVFSRDGQQIAFVEGLMSDEDSVGGDIFTIPAKGGKAQNVTPEMKASANWL
                     AWAPEGKILFAGSVEGDAQLATVDPASGRIESIWRGQEQVSAGLWGTNVSVASDGNTS
                     VVIRSSLSEPPEIWAGPIGYWRQVTHRNAAVKPAWGEAKSIHWKNDGYNVQGWLIYPR
                     DFDPSKKYPMIVVVHGGPGAAVQSEWPHSGSFAIALAGAGYFVFQPNPRGSFGQGEAF
                     TLANVRDFGYGDFRDVLVGVDEALRVAPIDPNRLGLTGWSYGGFMTMWGVTQTNRFKA
                     AMAGAGIANYLSYYGENKIDQWMIPFFGKSVYDDPEVYAKSSPITFIKNVKTPTLVIV
                     GDSDGECPTPQSYEFWHALKTLGVETKLVVYEHEGHMFVKPDHQRDRIRRTVAWFNAH
                     LR"
     gene            4334..5626
                     /locus_tag="VGR03_03805"
     CDS             4334..5626
                     /locus_tag="VGR03_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV1993437.1"
                     /translation="MIDRRGFLSSFAGAAGALGLTPKWEPLLEQEPLKLPDSALYGSN
                     EEAYWTELRKQFLIPTDEVYLNNGTVGSSPMPVLRAVFDGYNETEKMAQSDPEDYPIW
                     GYGAWNEFRDPLAEFIGCTRDELALVRNATEANSYIANGLDMKPGDEVLMTDQEHPGG
                     EQPWNLRAKRYGIVVKKITLPKPVPNAAAVLNLFNDAITPRTRVMFFSHIPTVTGVVL
                     PAKELCGLARSKGILSAVDGAHVVGMMRLNVHEVGCDMYTSSPHKWLQAPKGSGFLFV
                     RDEVIDRLWNTIVTAGWDEPKLRAERFQRIGSSNVPALWGLRASVQLANQIGMEKIER
                     RHRQMADYILKEMVQRGAESWTSPDPALRCAIAAVNVAPIQITEIENWMWKNKKIRIR
                     GGAPSKIRLSTPYYLLGNDVERFLSAYDEYRRNKGKVA"
     gene            5842..>6139
                     /locus_tag="VGR03_03810"
     CDS             5842..>6139
                     /locus_tag="VGR03_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993438.1"
                     /translation="MQNLKRLGIGLGILAILSPAVVAQAPAGSAPAAKEDRYVGPTMN
                     TSFDGSVDSGSQVYDWTTSAGYIFNRHFSASFGAPLYFVRGTTATGTTTSSTGIG"
BASE COUNT         1298 a         1678 c         1913 g         1250 t
ORIGIN      
        1 cggaccttgc ggcccgccca tccggttgaa ggttagctct tgggcgccca ggctgaagcg
       61 atcagctcct gagagaaggc cggcggcacc tgcttgaagc aggcgtagcg atagagcgcc
      121 gcgttgaaga taccccctac cgcggacatg aaaaccgaga gcaagaggaa ataaagaatc
      181 atcaaaacaa agccgacgag catgccgctg atactggcga gaaacgtggc ggcgatcaac
      241 agaccgatgc caggcagcat gagaaccata gaaacgaggc tgaggctgaa gccgccgatg
      301 accttttctc cccaggtgtc gcggaagagc ttggaggagc gcttgacggc ttcgatgggg
      361 gtgaggtctt cgaaggccag cacaggaacc acgaagtagc aggccagcgt ccaggcgatg
      421 ccgatgattc gcatgatcaa gcggccgatc aggcccaggc gttcttccat agtgcgcagg
      481 atgacgccga cggttgcggc gacgagcgcc cactgcagaa tcgtgccctt gcgctcccag
      541 gccagctcga tgccgtcgcg gaacgtccag gtgccgccca tcaggcggct attggcgacg
      601 ccgacgaggg caacgttgaa gaacacgatc acgaaatagt tcaccacgta caaagtgaac
      661 atgccgagca ggaacatcgg cgactggttc ggattaaacc gttccccggc ggccagggcc
      721 gcggccctcg ccggcagcag caggaacgcg ccgccagtgg cgataatggc cgtgacaata
      781 aaacacgaaa cggccgagag caccggaaac agcatcaatt gtttgtcgga acgaagaatg
      841 gcgaagctct gcttgacgag ctcccagcta cgagaaaact tacccatcgc accctcccac
      901 gcattcggaa acgacggaac tgtgacctgt ggatagtttg gcgcaaaggc atgaagattc
      961 caatagtacg ttcgatgcac tcccaacgga cagataacgt ttccgacaac ggttatcccg
     1021 gccggaaaaa ccgaagggtg cagcccccgc ggacttgcag tagctgcgct gttgtttttc
     1081 ggcgatgagc ttttccacgc gagcggcgac ttcaacgtgt ttgagcactt ccccgcagtc
     1141 gatctaggcc aacccttctt ccacctggcg gataaaccgc tggtcgtaac caatcaaacg
     1201 ctcgaccgct tcatgcacca gggatgcgct attgcgtccc tgctgggcgg caatgctgtc
     1261 cagcctcgct tgcaatccgg gcgaaagtat tacttccatg gggtcagtgt aagagctgct
     1321 ccgggggtct gtcatagtgt acatcctctt aagaggaatg catcctggga ctcgaatcgt
     1381 ccgcgcgatt tatgtccgtg ccacccgcca gtacttcaag ccgaattcgc cgcatcggca
     1441 gattcagaaa tcattcgacc gtggacgagc aacactggcg agcaaacgtc atggattgca
     1501 cgtgccgaag tccttggtga gcgtctctac ttgaaatgag ggctttccgc tggggtcttt
     1561 ggacttcttg ccttttaggg tgactcggtc gccagctttc agagctgcgg aaccccccga
     1621 cagggcgtag acctttttgt ccttttcact cttcagggtt aggccgtctg cgccggaggc
     1681 aacgcatcct gtgatggaga gatgcttgtg ggtttcatga tagatgaggt atcccactac
     1741 ggtgagagca gcggcaaccc ccgctactat ggcgatgatc tcgcctttgc tggggccgat
     1801 gttcccaccg gtttggggga aagccggaag cgcgagaagc aaacaaagag caagcgcgcc
     1861 gaatgtacga agcggggaac caagactcat aacggaatct ccttcttagc ttcaacaaag
     1921 gcaacggaat catacacccg ccccggctgt tcgcctgtgg gtcaaaacag agttataccc
     1981 ttgccggttc cacgctctgc agccgattcc ttgtcaattt ctctaagcaa tcgggcgggg
     2041 cacgctcata ttttacctgg tagtgattta gggaaatggg attctgccat cgcatgttca
     2101 agaaagccaa acaaagaatc acccacccgc tggcatcagc ggaggtgcgc attgaaccag
     2161 gcgacggtgc ggcggatgcg gtcgcgctgg tggtccggct tcacaaacat gtggccttcg
     2221 tgctcgtaga cgacgagctt ggtttcgacg cctagagttt tcagggcgtg ccagaattca
     2281 taggattgcg gcgtggggca ttcgccgtca ctgtcgccaa cgatgacgag cgtgggcgtt
     2341 ttcacgtttt tgatgaaggt gatgggtgag cttttcgcgt agacctcggg gtcgtcgtag
     2401 acggacttgc cgaaaaacgg aatcatccac tggtcgatct tgttttctcc gtaatagctc
     2461 aaatagttgg cgatgccggc cccggccatg gcggctttga agcggttggt ttgcgtgacg
     2521 ccccacatgg tcatgaaacc gccgtagctc cagccggtga ggccgaggcg gttcgggtcg
     2581 atgggagcga cgcggagggc ttcgtcgacg ccgacgagga cgtcgcgaaa atcgccgtag
     2641 ccgaaatcgc ggacgttggc gagcgtgaag gcttcgccct ggccgaaact gccgcgagga
     2701 ttgggctgaa aaacaaaata gccggcgccc gcgagggcga tggcgaagct ccctgaatgc
     2761 ggccattccg actgaaccgc ggcgcccggg ccgccgtgaa ctactacaat catgggatat
     2821 tttttcgagg gatcgaagtc gcgcggatag atgagccagc cctgcacatt gtagccatca
     2881 tttttccagt ggatgctctt ggcttcgccc caggcgggtt ttacggcggc gttgcgatgg
     2941 gtgacctgcc gccagtagcc gatggggccg gcccagattt ccggcggttc gctgagcgag
     3001 ctgcgaatga cgacggaggt gttgccgtcg ctggccacgg aaacgtttgt cccccagagg
     3061 ccggcggaca cctgttcctg gccgcgccag atcgattcga ttcggccgct ggccggatcg
     3121 acggtggcca attgcgcgtc gccttcgacg gatccggcga aaagaatttt tccttcgggc
     3181 gcccaggcca gccagttcgc ggaagctttc atctcgggcg tgacattttg tgctttgcca
     3241 cccttggcgg gaatggtgaa gatatcgccg ccgacggaat cttcgtcgct catcaggcct
     3301 tcgacgaagg cgatctgctg accgtcccgc gagaagaccg ggcgcgcgat ctgcagagag
     3361 ggtttgtaaa tgggcttcat caggcccagt agacctgcgt ccagcgtata gagttgggcg
     3421 acccaccaat tgttgtcgcc gttgccgtgc gcggccgtga ggacgaagcg cgtgccgtcg
     3481 ggcgaccagt cgtattcgta gacgtacata tccgcgggag agatttggcg gaagccgccg
     3541 gtagtgagtt caacgagagc gaggcgttgt tcgaagaaag cgtcttggat ctcgccggtt
     3601 tcgggcgttt cggcaacaag cgggccggcc gcgcgtgtgg cgttttcggt gtataggaca
     3661 gcgatagttt tgccatcggg cgaccacttg ggcacggcga gcaacccttt cgcgttcgtg
     3721 agcttcttgg ccggcccgcc aggattcgca acatagagct gaagttggcc gggctttgcg
     3781 gcgtcggaca agaatgcgat ttgcttgctg tcgggggacc aggcgacgtt tccctcggca
     3841 cgaggagcgc cactggcagc agccgtgatg cgttttggca tcgcgctggc ctcgcggtcg
     3901 gcgacgtaga tcgctgtgtt gctatcgggc gcgccgtctt tgccgacgag ggtctcgacc
     3961 caggcgagtt tctttccgtc gggcgaaatg gcagtttgtt cgaatgtgtg tgccgcgtag
     4021 agtgcgtcga tgacgttatc gaggggtgag ccggcttttg gtttctgcgc cggctgcgag
     4081 tgcgccgtga ccgcctgctt cgcagtgctc ttcgcttgcc cggagatgta gcttgcatta
     4141 cagagagaaa aagtgaagat gaccgcaacg atccatttcg aaaagcgcat gtctaccgac
     4201 ctttcgtccc gaagtgacgc cgcgcaaaga tagacctacc tgcgcgccgc tgcaaggctt
     4261 gggtttcgaa tgtttgttgt tgtgggcgaa gttcccacgt agaatccgcg caactcgcct
     4321 tgtggaggga accatgattg accgccgtgg ttttttgtct tcgtttgcgg gcgctgccgg
     4381 tgcgcttggc ttgacaccga agtgggagcc gctcctcgaa caggaaccgc tgaagctgcc
     4441 ggacagcgcg ctatacggtt cgaacgaaga agcgtattgg acggaattgc gcaaacagtt
     4501 tctgattcct accgatgagg tttacctgaa caacgggacg gtgggatcgt cgccgatgcc
     4561 ggtgttgcgc gcggttttcg acggctataa cgaaacggaa aagatggcgc agagcgatcc
     4621 ggaagattat ccgatctggg gatatggcgc gtggaacgag tttcgcgatc cgctggcgga
     4681 gttcatcggc tgcacgcgcg atgaactcgc gctggtgcgc aacgcgacgg aagcgaacag
     4741 ctacatcgcg aatggtcttg atatgaagcc cggcgatgaa gtgttgatga ccgaccagga
     4801 gcatccggga ggcgagcagc cctggaacct gcgcgcgaag cgatacggga ttgtggtgaa
     4861 aaagatcacg ctgccgaagc cggtgccgaa cgcggcggcg gtgttgaatc ttttcaatga
     4921 tgcgatcacg ccacggacgc gcgtgatgtt tttcagccac attccaactg tcacgggcgt
     4981 cgttttgccg gccaaggaac tttgcgggct ggcacgttcg aaggggattc tctccgccgt
     5041 ggacggcgcg cacgtcgttg gaatgatgcg gctgaacgta cacgaagtgg gctgcgacat
     5101 gtatacctcc agcccgcaca agtggctgca agcgccgaag ggcagcgggt ttctcttcgt
     5161 gcgagatgaa gtgatcgacc ggttgtggaa caccatcgtc actgccgggt gggatgaacc
     5221 aaagctgcgc gcggaacgct tccaacggat cggcagctcc aacgtgccgg cgctgtgggg
     5281 cttgcgcgcg tcggtgcaac tggcgaatca gatcgggatg gagaagatcg aacggcggca
     5341 ccggcagatg gcggactaca ttttgaaaga gatggtgcaa cgcggggcag agtcctggac
     5401 gtcgcccgat ccggcgctgc gctgcgcgat tgccgcggtg aatgtggcgc cgattcagat
     5461 cacggaaatc gaaaactgga tgtggaagaa caagaagatc cgcatccgcg gcggcgcacc
     5521 atcgaagatc cggctttcga cgccgtacta tttgttgggc aacgatgtgg aaagattcct
     5581 ctcggcctac gacgaatacc ggcgaaacaa aggcaaagtg gcatagtttc aggcaggtat
     5641 tctggcggtt tgacgaactg tccgaagttc tctacgagag tagcttttac acagttacaa
     5701 gccgtagttc tcccggaaaa ccgttctatt ccattcaaaa taaataagta agtggaagcc
     5761 agccttcggc gggcaggatg catactacta cccagcgcaa cgagtatcgg tcataaattc
     5821 tttcatggag ccataacaaa cttgcaaaat ctaaaacgcc tcggtattgg cctcggaatc
     5881 ttagcgattc tcagtccggc tgtagtcgcc caagcacctg ccggctcggc ccccgcagcc
     5941 aaagaagacc gatatgtggg cccgacgatg aacacttcct tcgacgggag cgtggattcg
     6001 gggagtcaag tttacgactg gacgaccagc gcggggtaca ttttcaaccg tcatttctct
     6061 gcgagtttcg gcgcgccatt gtactttgta aggggtacga ccgccacagg cacgacgact
     6121 tcctcgaccg gcatcggca
//
LOCUS       DASYVO010000115        17394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_159400, whole genome shotgun sequence.
ACCESSION   DASYVO010000115 DASYVO010000000
VERSION     DASYVO010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 17394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17394
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159400"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VGR03_03815"
     CDS             complement(<1..294)
                     /locus_tag="VGR03_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993439.1"
                     /translation="MGAKPKLDPSLSPVVDRAVERAKPGWPGLPPGTPNPEETYRRIA
                     CDVLETAASMGLRIAEAPGKPPEAQEADELLARIGQHAGDIRALRQLWLTRDPQ"
     gene            complement(559..786)
                     /locus_tag="VGR03_03820"
     CDS             complement(559..786)
                     /locus_tag="VGR03_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993440.1"
                     /translation="MATKIKAKKAKRPGKSLDRISVIKGVKKAPASKGEVAGHTSPGA
                     YYLCWNCGRLNWVPWGWNYFHCWYCGALNLV"
     gene            1067..1468
                     /locus_tag="VGR03_03825"
     CDS             1067..1468
                     /locus_tag="VGR03_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1993441.1"
                     /translation="MSGSTISTFELFQLFSDAETARKYLESRLWPNVVKCPTCKAGER
                     IGVRKDGFYRCNGCKLDFTVRTGTVFERSHVPLHKWVYAMYLLVISHKGISSIQLAKE
                     IGITQKSAWFVLKRLREACGNDLTMLRGLLG"
     gene            complement(1892..3775)
                     /locus_tag="VGR03_03830"
     CDS             complement(1892..3775)
                     /locus_tag="VGR03_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1993442.1"
                     /translation="MSKIRLILQFGLKLVRLSPLYVAAYVLLNLVSQTAIPLMLPVLI
                     GKLTNSVQPAGPAAGKTSAPQTQSPGAASKPTTEASRSSGSAEGAGGRGDSVTAYYEW
                     LALTVLFIPLSICFRLSQTKMDGKMEKEVRQSLFDKVIRQAPEFFHRYNPGELTNVLT
                     QTSIEAQQALRSLTVDPLLQLASLTIAMALIVQQLRHINGPFIWPAVGLMIVFGVGSA
                     WLIQRKSDKMVGKSQREAQMQRFALAGLADAAVKSAEEIQAMDAEQVFSSRHDEALNR
                     MMKLKTGQTLTMEILNSAIGVPTQIIVACLFGLVVYQSVARTSGIPPGVFVTIAGLAP
                     QLMQPFKSFAVLGVIASASWPAIELVTRMLKEENRIKDLPGARDIEKVAPTLEARRIK
                     FGYVPGERKVFDDLTFSVPAGGITGFVARMGQGKTTFFRLAMRFYDPESGEILFGGFP
                     TSSFTLRSLRRCAVIMSQFPAFFHDSVGENFRVAKPDATDEEIRALCERTRLWPILER
                     AVGKDPLDKPFAAGMGISGGQKKLFALTRCLLRNPTFLFLDEPTTNMSNDEKYLLIPL
                     MREACAGKTVIVVDHDLSWLTQFCDHFVVLDSGKIVQHGTAAELLAVPGVLNELYSLA
                     HPA"
     gene            complement(3898..4686)
                     /locus_tag="VGR03_03835"
     CDS             complement(3898..4686)
                     /locus_tag="VGR03_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV1993443.1"
                     /translation="MPPWIDLKDAKRALAEGTGKGIRVAVLDSGVEASHPKLGITLLD
                     DLAVVAEGHKLRVVKGGGRDTFGHGTAVAGVIHELAPQAKIGSIRVLGHDNTSRTSII
                     ERAAQEALDRGYHIINCSFGCGVENQVLQYKKWVDEAYLKGIHIVAACDNIDFSKTEW
                     PACFPSVVAVNMAETDDGKIFYYQRGTLVEFAARGVNVPVLWAGGAEKVVTGSSFAAP
                     RLSAMLARLLSGCPNVTPLEAKAIFHKLALPWTHKVAAQNVMIG"
     gene            complement(4700..5341)
                     /locus_tag="VGR03_03840"
     CDS             complement(4700..5341)
                     /locus_tag="VGR03_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV1993444.1"
                     /translation="MRKPGALAMLSDAKMPQSRLTLLPDERLAPALPALQECLSSAGD
                     SIAAENLASMLDETMKRVIHLAFQQAGADEGTVWLLDESKEALIPAYNTGPNADQFVG
                     HFRQPLSAGLVSMVFGNEKPFTENEVPKNAKQDKRLDTILKTQTLAMIIVPLYFLDAC
                     RGVISCVKLGVPGAKARDAQGFSDSAQGLMVHGATLLGRLIDLRVLRISLGLR"
     gene            5509..7026
                     /locus_tag="VGR03_03845"
     CDS             5509..7026
                     /locus_tag="VGR03_03845"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEV1993445.1"
                     /translation="MGYLPEFDDDVFISYAHNDDIPRRDLDEKEGWVAQLHANLEGIV
                     RELLGQEPSLWRDCEISANDDFTRKITNRLAKTATLLSVITDNYLEREWCQRELAEFC
                     RHAESKIGLRVGEKRRIFKVLKREIRSQNLPEQFDGMERYEFYGEDTEDPKKSVHEYR
                     PSLGGLQKRLYYLRMNDLAEDIADTLRVMRAAAQGATVAAPQGGTVFLAETSADQDFS
                     RNCIKKELQKRNIAVLPQGEIPFRGMQFEQHVSEYLQQSDLSIHIFGNRSGVVPEGSE
                     SPHTWLQHDLAMQRANDPDFLRVVWLPKDLEPADEKQRQYLQHLRNDPAVQKGAEILE
                     VGLEDLKTEVLHSLDRLQRKKVEPPPQPTASDEPLKVYLICDSQDLQSPSLLALDNFL
                     FDQGFEPKKSQLGDNPEDSRTLHEDYLQFSDAYLIYYGSASEAWVQAKLNDFRKLGGK
                     RQTPVLAKAIYIAAPLNDAKRAFRSHEAKVIQGGPDFNPASLAEFVETVRKPKGR"
     gene            7030..11349
                     /locus_tag="VGR03_03850"
     CDS             7030..11349
                     /locus_tag="VGR03_03850"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993446.1"
                     /translation="MVAEDSRFNPFPGLRPFQYGEQHLFFGREGQSAEILKRLREHRF
                     LAVVGSSGSGKSSLIRAGLLPDLHGGFLAGAGSHWRIAVFRPIDDPIGNMARALNKPD
                     VLQTEPPSGGDQGRSDILLEVTLRRSGLGLIEAARRAHLPENENLLVVVDQFEELFRF
                     ANTASAPSQADDATAFVKLLLEATQQTELPIYVVITMRSDFIGDCARFRDLAESVTAG
                     MYLVPRMTREQRRSAIAEPVGVGGGCISLRLINRLLNEVGDNPDQLPILQHALMRTWG
                     QWENRTRGAASFEKDPIDFADHDAIGGLAKALSNHADEAYNALPDDRHREIAKRMFQC
                     LTEKGADNREIRRPTSVARLAAVASASAGEVIEVINEFHLQGRSFLTTPSKEDLSGDS
                     VIDISHESLIRLWTRLVGWVEEESESARMYERLADAAALYAQGKGSLWDGPSLQNALD
                     WKKDNQPTAEWAQRYNPQFEEVMAFLDKSCQAHEEAEKEKKRRRDMELRRTRRNLAVV
                     SVFSVVALGLAAFGWFMWDRAGLAEQLAKAKEQEAVRSKAIAESKGQEAAEKGALAEK
                     SAEALRIQALRVQQEHLGVVGNRISLLDSLIEKSTPYEAAEWHREKASLLIEQNLLED
                     AVKEAKVAIDLAPNNLEARTDRGYAQMLLNNPGEALVDFEYIRDKINGKLTINYLNLT
                     IALAQLDREADASNSLKQAIYYSLHGENNGGSEALVPPEIQDATGRTALIANGEIFHQ
                     ALYFLKPLLGAYAGRKDFVSDLEAAKEHAKQDRIRPNEREDAGLIALTWAWFLERMNT
                     PNPKPKPKYGLLAGQGALWDEAGYPEYAARHFDAFLRKHAEQKDTRYDDLAEWVRGER
                     KKLGSGESVGKKPDSTEMEVKSDLLTAEKKYSDAEKIISEAIVLEPSNVRLYFKRMSI
                     RFREANRVDSDYRKAKAKADEDKGRITVLEKPASTSQAKTPASEQLEENKAKQTPEEQ
                     EKARQEELKTLKADEAKQRKTADSAKQKERQLYQGVIKDCETVLKLRKDTPNAYFYRA
                     VANFRLEKAQDSIIQDLKHAIDLDPTHLAAMDWLAGPSIKTPTEEAARLRLRYQQLFP
                     GSDWNLWQLAELQIKMKQFANAYDTIQKAIAIEPVDYAYYQTRADAEKGLGYSEAHIQ
                     RDRAEGNRLAVEFLRRKGNKEDLDKANDWEQDEWKILSELGDKSASGEVRCNSDVTTC
                     LSLQVTHGYGEWAIFGITSVQMADQDTRIVQIKQGGNDGIVLGSAGSVFSLYSNEKEH
                     ERTVANIGRAEVLSVEPQSALLRVKMPKPEGDGMVRAGDGVQLLVRTPKIEHRSKLWS
                     VVKFNISLEDLNGKKIAGFDTLYSKESPELDRAIYQKLLDDIQEVGRQFGDKWDEGKP
                     ISKGKFAGKPTRLRQALEKTSREDLDGFFQYVVKFPATYFGHNWRIGGIYAYWVLLGM
                     PEK"
     gene            11612..12298
                     /locus_tag="VGR03_03855"
     CDS             11612..12298
                     /locus_tag="VGR03_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV1993447.1"
                     /translation="MQRRALIVVDEPATSMLIEKALQSVGMEALTAPHSAVAAASLRE
                     GRFDLVFLDSHMTPGDGIELVRRFRSSQLHLTTPIVLISDDKKPGAMAKGFEAGASFF
                     LYKPVEKRNVLQLVRATQGTIEHERRRTRRVPVHSKVHLRNGQGELEGETVNMSMNGI
                     LVRAPFTFPVWSSLDVRLYLSPSARPIIGAGRVARISGPNQMGIHLGQLSSAESQRLQ
                     EFLLPLIPAD"
     gene            complement(12496..12572)
                     /locus_tag="VGR03_03860"
     tRNA            complement(12496..12572)
                     /locus_tag="VGR03_03860"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12536..12538),aa:Asp,seq:gtc)
     gene            complement(12593..12669)
                     /locus_tag="VGR03_03865"
     tRNA            complement(12593..12669)
                     /locus_tag="VGR03_03865"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12633..12635),aa:Val,seq:tac)
     gene            12812..13486
                     /locus_tag="VGR03_03870"
                     /pseudo
     CDS             12812..13486
                     /locus_tag="VGR03_03870"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
     gene            complement(13557..14648)
                     /locus_tag="VGR03_03875"
     CDS             complement(13557..14648)
                     /locus_tag="VGR03_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEV1993448.1"
                     /translation="MGKSIRPILSLTLVVALLGWPTIGVAQSPQPPAPSQPQTQQPQQ
                     QPVPQQTQVNPKNSKEDVDAIGNRNVGKGINFYSLEREIAIGKQLAQEVERSAKLIDD
                     PVVTEYVNRVGQNLVRNSDAQVPFTIKVIDSDVVNAFALPGGFFYVNSGLVLRAQEES
                     ELAGVMAHEISHVTARHGTKNATKGQIMQLATIPLILLGPGGWAGYAIYQGLNLAIPL
                     TYLKFSRNDEREADFLGLQYMYKAGYDPNSYVTFFERVQADERRQPGTVAKVFSSHPP
                     TPDRIANAQKEIARILPARDEYIVTTSEFDQVKARLRGIMFTRKVLENDPNKPTLRSR
                     TEKDKKKPQTTDTGTDDDRPTLKRRPDSP"
     gene            14821..15042
                     /locus_tag="VGR03_03880"
     CDS             14821..15042
                     /locus_tag="VGR03_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993449.1"
                     /translation="MSARKVQIGQLWKREATGETYLVTRVYSEALSTMVVLRKSGAEQ
                     EAIIRVRVERLGDGQTIPGFSPAQEDEKF"
     gene            complement(15063..15779)
                     /gene="bshB1"
                     /locus_tag="VGR03_03885"
     CDS             complement(15063..15779)
                     /gene="bshB1"
                     /locus_tag="VGR03_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEV1993450.1"
                     /translation="MKLDLLAIAAHPDDVELTCGGTLLKMAKRGYKTGILDLTAGEMG
                     TRGTPETRAKEAARAAKLLRVKWRGNLGMPDSDVQPSRQHKLRLAQTLRELRPRTVIL
                     PYWEARHPDHYHASTLGYEACFLAGLKQLPIAGEAFRPFKILYSTAFTEVRPTFVVDI
                     TAEFEVRHRAILAFASQFLPSGKARRGEVHLAIERLEEDMSLLARRYGLMIGVKYGEP
                     FLTKELMQVEDIVEMPVRSI"
     gene            15865..16614
                     /locus_tag="VGR03_03890"
     CDS             15865..16614
                     /locus_tag="VGR03_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1993451.1"
                     /translation="MPDAPSTIPVSLQGRVALVTGAGRRIGRAVALRLAREGADVVVH
                     YRGSKAEAAQVVSEIEALGRRAVALSADLREVKSIHRLFSEAARQFGRLDILVNNAAV
                     FFPARVENATEEQWNAALDTNLKAQFFCAQAAAPALKESRGVIVNFASIGGLLPWPGY
                     IPYCVSKAGVIMLTRCLARALAPEVRVNAVAPGTISMPGDPPEWQADYIRLAPLKRTG
                     TPDDIADAVSFLIHSTFMTGQVLVLDAGRTL"
     gene            16901..>17394
                     /locus_tag="VGR03_03895"
     CDS             16901..>17394
                     /locus_tag="VGR03_03895"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV1993452.1"
                     /translation="MIGQTISHYRIVEKLGGGGMGVVYRAEDLKLGRFVALKFLPEEV
                     AKDAQALSRFQREAKAASALNHPNICTIYEIDDQHGQAFIAMEFLDGATLKHHIAGRP
                     LELDTLLAVSIEIADALDAAHVQGIIHRDIKPANIFVTKREHAKILDFGLAKLTAEKV
                     TPLEL"
BASE COUNT         3642 a         5360 c         4845 g         3547 t
ORIGIN      
        1 ctgcggatcc cgcgtcagcc acaactggcg cagggcccgg atgtcgccgg cgtgctggcc
       61 gatgcgagcg agcagttcgt ccgcttcttg ggcttccgga ggcttgcctg gcgcttcggc
      121 gattcggagg cccatcgatg cagccgtctc caaaacgtcg caagcgattc gccggtaggt
      181 ctcttccgga ttgggcgtac cgggtgggag gcccggccag ccgggctttg ctcgctcaac
      241 tgcgcggtca acgaccggcg agaggctggg atcgagtttg ggtttcgctc ccatgcgcgc
      301 aactcatgtc tagaaaggag taccggccaa acaggaccgc cgagaagaaa gcacttgaaa
      361 aactttccga atgaaaaccc tcgagtccgt cgagccccaa tttgccgctt tataaggaag
      421 caggccccga ggtttcctgc tcgggacctg ctccatgttc ctgccgccgc ctatcgcgca
      481 ggccaattag cgcgaagctt gatcggcggc gtcgtccatt tcaactcagc ccttgcggag
      541 actgcccttg tcgggtcttt ataccaggtt cagcgcgccg caataccaac agtggaaata
      601 gttccaaccc cagggtaccc agttcaaccg gccgcagttc caacagagat aatacgcgcc
      661 cggagaagtg tggccggcca cttcgccctt gctggccggg gcttttttca cgcccttgat
      721 gacgctgatt ctatcaaggc tttttcccgg cctcttggcc ttctttgctt ttatcttcgt
      781 cgccattgct tcgtctcctt ttaacagtga tggctgagtt ccgttcagcg ccttcttggg
      841 gaccgtcgaa aacgtttcat ccgttccttg cagaatgcgc cgaactttca actcgcagag
      901 gatactgacc tgcgcccgtt cccttcaatc ctcgaagaag acctgcgcaa atcggcttat
      961 ctatattcga agtgcgtgat acctgtcaag gaaaacttat tgtggcacta tatacttgac
     1021 tccctcgccc gtttaaggca taacttccct cagggagaaa gtcacaatga gcggaagcac
     1081 cataagcacg tttgaactgt tccagttgtt ttccgacgca gagacggcca ggaagtatct
     1141 tgaatctcgc ctctggccga atgtcgtcaa gtgtccaaca tgcaaagcgg gagaacgcat
     1201 cggggttcgc aaagacggtt tctatcgctg caatggctgc aagcttgatt ttacggttcg
     1261 caccggaacg gtttttgagc ggtcccatgt tcccctgcac aagtgggttt atgcgatgta
     1321 cctgctggtg atttcgcaca aaggtatttc ttcgatccaa ctggcgaagg aaatcggcat
     1381 cacgcaaaag tccgcgtggt tcgtgttaaa gcgtttgcgc gaggcttgcg gcaacgacct
     1441 aacgatgctg cggggtctct tggggtagac gaaacctatc ttggcggaaa agaaaggagc
     1501 gagcactcag caagaaaccg gggaatagca tatcagccgt tatgtcaacg agtgcacttt
     1561 ccggttcaac gatgccaatg tggtgcggca tactctggac aggctcaaca gttttgtgga
     1621 tgctaccctt ggccaacagt tcacctataa ggatttgacc gcatgaagga ttttgtggag
     1681 atagtggaca gggtcgtgga ggtagttctt gcctacagac cgaagaaaaa caaacgacga
     1741 aaatctagga aatcccgcaa gaaaacgaag cggagaaaag tagttgcaag atgatgacaa
     1801 cgacggggcc gagaagcctt agagggattc aagtatatga atccccttca ttgaaagacc
     1861 gtggaccggt ctccgttttc ggggtcacgg gtcaggccgg gtgcgccagc gagtagagtt
     1921 cgttgaggac gccaggcacc gccagtaatt ccgcggccgt gccgtgctgg acgatctttc
     1981 ccgagtccag caccacgaag tgatcgcaga attgtgtcag ccaggaaaga tcgtggtcca
     2041 ccacgatcac cgttttcccc gcgcaggcct cgcgcatcag cgggatcagt aaatactttt
     2101 catcgttgct catgttcgtt gtgggctcgt ccagaaacag gaatgtgggg tttctgagca
     2161 ggcatcgcgt cagggcgaag agttttttct gtccgcccga aattcccatt ccagccgcaa
     2221 acggcttgtc caggggatct tttcccacgg ccctttcgag aatcggccac agtctcgttc
     2281 tttcgcagag cgctcgaatc tcttcgtcgg tcgcgtcggg ctttgccacg cggaaatttt
     2341 ctcctacgct gtcatgaaag aaagcaggga actgagacat gatcaccgcg caccgccgca
     2401 aactgcgcag cgtgaacgag ctcgtcggaa agcctccgaa caggatttcg ccggactcgg
     2461 ggtcgtagaa tcgcatcgcg agccggaaaa acgtcgtctt gccttgcccc atccgcgcca
     2521 caaaccctgt aattcccccc gccggcacgc tgaaagtcaa gtcgtcaaac acttttcgct
     2581 ctcccggaac gtaaccaaac ttgatgcgtc gcgcctccag tgtcggtgcc accttttcga
     2641 tatcccgcgc gcccgggagg tccttgatgc gattttcctc tttcagcatt cgcgtcacca
     2701 gctcgatcgc cggccaggac gcgctcgcga tgactcccag caccgcgaac gatttgaacg
     2761 gctgcatcaa ctgcggcgcc aatcccgcaa tcgtcacgaa cacccccggg ggaatgccgc
     2821 tcgtccttgc gacggactgg tacacgacga gcccgaagag gcaggcaact atgatttgcg
     2881 tcggaactcc gatcgccgaa ttcaatattt ccatcgtgag tgtctgtccg gtcttcagct
     2941 tcatcattcg attcagcgcc tcgtcgtgcc ggctgctgaa aacctgctcg gcatccatcg
     3001 cctgaatctc ttcagccgac tttacggcgg cgtcggccag cccggcgagc gcgaaacgct
     3061 gcatttgggc ttctctttgc gacttcccca ccatcttgtc gctctttctt tgaatcagcc
     3121 acgccgagcc aaccccgaag acgatcatca aacctaccgc cggccagatg aagggtccat
     3181 tgatgtgccg cagctgctga acgatcagcg ccatggcgat cgtcaggctc gccaattgaa
     3241 gcagtgggtc cacggtcaga cttctgagcg cctgctgcgc ttcaatgctt gtttgcgtca
     3301 gcacgttggt gagttcgccg gggttgtagc gatggaagaa ttcaggcgcc tgccggatca
     3361 ctttgtcaaa gagactctgc cgcacctcct tttccatctt tccgtccatt ttcgtttgcg
     3421 aaagccgaaa gcagatgctc agcggaatga acagcactgt cagtgcgagc cactcatagt
     3481 atgcggtgac gctgtctccc cggccgcccg ccccttccgc tgaaccgctc gagcggctgg
     3541 cttccgtcgt gggtttggac gccgctcctg gactttgcgt ctgaggtgcg gaggttttcc
     3601 cggctgcagg cccggcaggc tgaacgctgt tcgtcagttt cccgatgagt accggcagca
     3661 tcaacgggat tgccgtttga cttacaagat tcagcagcac gtacgcagca acgtaaaggg
     3721 gagaaagccg aaccagcttc aacccaaact gcaggatgag gcgaatcttg ctcatcgaga
     3781 gcggggtatc ttgcgccccc gggggcttcc ccggcggtgt ctggcccggt gcctggggac
     3841 cgggcccctt cccaggcgct tgttgtgcgg cttggccagc ttgtggtggc atgtgtttca
     3901 cccaatcatt acgttttgcg ctgcgacttt gtgcgtccag ggcagggcca gtttatgaaa
     3961 aatcgctttc gcctccagcg gcgtcacatt cggacagccc gaaagcagac gcgccagcat
     4021 cgccgaaagc cgcggcgccg cgaagctgct gccggtcacc actttttccg cgcctcccgc
     4081 ccacagcacc gggacgttta cgcctctcgc cgcaaactcc acgagggtcc cgcgctggta
     4141 atagaaaatc tttccgtcgt ctgtctctgc catattcacg gcgacgaccg agggaaaaca
     4201 cgccggccac tccgtcttgc tgaaatcgat gttgtcgcac gccgccacga tatgaatgcc
     4261 cttcaagtac gcttcgtcca cccatttttt gtattgcagc acctggtttt cgaccccaca
     4321 tccgaagcta caattgataa tgtgatagcc gcggtccaaa gcctcttgcg ccgcgcgctc
     4381 gataatgctc gtgcgcgagg tattgtcgtg cccgagcacg cggatgcttc cgatcttcgc
     4441 ttgcggtgcc agctcgtgga tcactccggc taccgccgtt ccgtgcccga aggtgtcccg
     4501 cccgcctccc ttcacgaccc ttaatttatg tccttccgcc accaccgcca ggtcatcgag
     4561 cagcgttatc cccagcttcg ggtgcgacgc ttccacgccg gaatcgagca ccgccactcg
     4621 aattcctttt cccgtgccct cggctaacgc gcgttttgca tccttcaggt cgatccaggg
     4681 tggcatgacg aatttgctct tacctcagcc ccaggctgat tcgcagcacg cgaagatcga
     4741 tcaatctccc gagcagggtc gccccatgta ccatcaatcc ctgcgcgcta tccgaaaatc
     4801 cctgcgcatc ccgagcctta gcgcctggaa cgccgagctt cacgcaagag atcaccccgc
     4861 ggcacgcgtc caggaaataa agcggcacga tgatcatcgc cagcgtctgc gtcttcagga
     4921 tcgtatccag cctcttgtcc tgctttgcat tcttcggaac ctcgttttcg gtgaacggct
     4981 tctcgttccc gaaaaccatg ctcaccagcc ccgcgctgag cggctggcgg aaatggccga
     5041 caaactgatc ggcgttcggc ccggtgttat aggcgggtat cagcgcctct ttcgactcgt
     5101 cgagcagcca caccgttcct tcatccgcgc ccgcttgctg gaacgccagg tggatcacgc
     5161 gcttcatcgt ttcgtccagc atggacgcca gattctcggc ggcgatgcta tcaccggcgg
     5221 aggaaaggca ttcttgaagc gcagggagcg ccggtgctaa tcgctcgtcg gggagcaacg
     5281 tgaggcggga ttgcggcatt ttcgcgtcgg agagcatagc gagcgcaccc ggctttcgca
     5341 agctcgattt ccagccgccc gtaggctcgc cgcaagaact ttgcggtcag cgaaaacgta
     5401 cgcgcacttc gagttgacac cgctaataac aaacgatata agcagttcgc acggtcttcc
     5461 gcgctattct gcggccccgc gaagcgatac aatccaaagg aggattgtat gggctacctc
     5521 cccgagtttg atgacgacgt tttcatcagc tacgcgcaca acgacgacat accccggcgc
     5581 gacttggacg aaaaggaagg atgggtcgcc cagcttcatg ccaatctcga gggtatcgtc
     5641 cgcgagctct tgggtcagga gccttctctc tggcgcgatt gcgaaatcag cgccaatgat
     5701 gacttcacaa ggaaaatcac caaccggctc gccaagacgg ccactcttct ttctgtgatc
     5761 accgacaact atctcgagcg cgagtggtgt cagcgcgagc ttgcggagtt ctgccgccac
     5821 gcagagagca agattggact tcgagtcggg gaaaaacgca ggatcttcaa ggtcttgaag
     5881 cgcgaaataa gaagccagaa tttgcccgag cagttcgacg gcatggaacg gtacgagttc
     5941 tacggagagg ataccgagga tcccaagaag agcgttcacg aataccgtcc ctccctcggc
     6001 gggttgcaaa agcgccttta ctacctcagg atgaatgacc tcgcggagga tatcgcggat
     6061 accctcaggg tcatgcgggc cgcggcgcag ggcgccacgg tcgcagctcc ccagggcggc
     6121 accgtttttc tcgccgagac cagcgccgat caggactttt cccgcaactg catcaagaaa
     6181 gaacttcaaa aacgcaacat cgccgtcctg ccccagggcg aaatcccctt tcgcggaatg
     6241 cagttcgagc agcacgtcag cgagtatctc cagcagtccg atctctccat ccacatcttt
     6301 ggaaaccgct ccggtgtcgt tcccgaaggc agcgaaagcc ctcacacttg gctgcagcat
     6361 gacctcgcca tgcaacgcgc gaacgatccc gattttcttc gcgtcgtctg gctgcccaag
     6421 gatctggagc cggccgacga gaaacagcgc caatacctcc agcaccttcg gaatgacccg
     6481 gccgttcaga aaggtgcaga aatccttgaa gttgggctcg aggatctgaa gaccgaggtt
     6541 ctccactctc tcgatcggct ccagcgtaag aaggtagagc cgcctcccca gccaacagcc
     6601 tcggacgagc cgctgaaggt ctacctgatt tgcgattcac aggaccttca aagcccttca
     6661 ctcctggcac tggacaactt cctcttcgat cagggcttcg agcctaaaaa atctcagctc
     6721 ggtgacaatc ccgaagactc ccgcacattg cacgaggact atcttcagtt cagcgacgct
     6781 tacctcatct attacggttc cgcttctgaa gcctgggtcc aggcgaagct caacgacttc
     6841 cgaaagcttg gcggcaagcg gcagactccc gtgcttgcaa aagccatcta cattgctgct
     6901 cctctgaatg atgcgaagcg cgctttccgc tctcacgagg cgaaagtgat ccagggcgga
     6961 cctgatttta atcctgcttc gctggctgag ttcgttgaaa ccgtgagaaa acccaaaggg
     7021 cgctagcaca tggttgcaga agactcgcgg ttcaatcctt tccccgggct gcgcccattc
     7081 caatacggtg agcaacacct tttctttggc cgggaaggcc agagtgcgga aatcctcaag
     7141 cgtctccgcg agcaccgctt tcttgcggtt gtcgggtcct ccggcagcgg aaagtcctcc
     7201 ctgattcgcg ccggacttct tccggatctt cacggaggtt tcctggccgg agcgggttcc
     7261 cactggcgca tcgccgtctt tcggcctatt gacgacccca tcggcaacat ggcgcgcgcc
     7321 ttgaacaaac cggatgtgct ccaaaccgag ccgccctctg gcggcgatca gggtcgcagc
     7381 gacattcttc tcgaggtgac tcttcgccgc agtggcctcg gcctgatcga agccgctcgc
     7441 cgcgcccacc ttcccgaaaa cgaaaatctt ctcgtagtcg tcgatcagtt cgaggaactg
     7501 ttccgtttcg caaacaccgc cagcgcgccg tctcaggccg atgacgcgac tgcgttcgtg
     7561 aagctccttc tcgaagccac gcagcagacc gaactcccca tctacgttgt catcaccatg
     7621 cgctccgatt tcatcggcga ttgcgccagg ttccgtgacc tcgccgaaag cgtgaccgcc
     7681 ggcatgtacc ttgttcctcg catgacccgc gaacaacgcc gcagcgccat cgcggagccc
     7741 gtcggtgtcg gcggtgggtg catttcgctt cgtctcatca accgcctgct gaacgaagtc
     7801 ggcgacaacc cggaccaact ccccattctc cagcatgccc tcatgcgcac ctggggccag
     7861 tgggagaatc gtacgcgcgg tgctgcatcc ttcgaaaagg atcccatcga ctttgccgac
     7921 cacgatgcca tcggcggcct cgcaaaagcc ctttccaatc atgccgacga ggcttacaac
     7981 gcgctgccgg acgatcgcca ccgagaaatc gccaagcgca tgttccaatg cctcacggag
     8041 aaaggcgccg ataaccgcga gattcgccgc ccgacctctg tcgcgcggct cgctgctgtc
     8101 gccagcgctt cggcgggcga agtcattgaa gtcatcaatg aatttcactt gcaggggcga
     8161 tccttcctca ccactccgtc aaaagaggac ctgagcggtg attccgtcat cgatatctcg
     8221 cacgaaagtc tcatccgcct ctggacccgc ttggttggct gggtggagga agagtccgag
     8281 tcggcccgga tgtacgaacg tctcgccgat gccgccgccc tgtacgcgca gggcaagggc
     8341 agtctctggg atggcccctc cctccaaaac gctctcgact ggaagaaaga caatcagccc
     8401 acagccgagt gggctcagcg ctataacccc caattcgaag aggtcatggc tttcctcgac
     8461 aagagctgtc aggcccatga ggaagccgaa aaggaaaaaa aacgccggcg cgacatggaa
     8521 ctccgccgca ctcggcggaa tctcgccgtc gtttccgtct tctccgtcgt cgcgctcggc
     8581 ctcgccgcct tcggctggtt catgtgggat agagccggcc tcgcggagca actggcgaaa
     8641 gcaaaggagc aggaggcggt caggagtaaa gcgatagcgg agagcaaggg tcaggaggcc
     8701 gcggaaaagg gagcgctggc cgaaaaatca gcggaggcgc tcaggattca ggcgttgagg
     8761 gtgcagcaag aacatctggg tgtggtgggc aacaggattt ctttgctcga cagccttatc
     8821 gaaaagtcta cgccttacga agccgcggaa tggcaccgtg aaaaggcatc actgctgatt
     8881 gaacagaact tgcttgagga tgccgtgaag gaagcgaagg tggccatcga cttagccccc
     8941 aacaacctgg aggctcgcac ggaccgcgga tacgcccaga tgcttctaaa caatcccggg
     9001 gaggccctgg tagattttga atacatccgc gacaagatca atggcaaact cacgatcaac
     9061 tacttgaatc tgaccattgc gcttgcgcag ctggacaggg aagcggacgc gagtaattcc
     9121 ctcaagcagg ccatctacta ttccttgcat ggtgagaata acggcggttc ggaagccctc
     9181 gttcctccgg aaattcagga cgcaaccggc cggaccgctc tcatcgctaa cggcgaaata
     9241 tttcaccagg cgctctattt cctgaagccg ttgctcggcg catacgcggg aaggaaggac
     9301 tttgtatccg atctggaggc agcgaaggaa cacgcgaagc aggatcgcat tcgcccgaac
     9361 gagagggaag acgccggcct catcgccctc acttgggctt ggttcttgga acgcatgaat
     9421 acgcctaacc ctaagcctaa gcctaagtac ggactcttgg ccggacaagg agctctttgg
     9481 gacgaagcgg gctatccgga atacgccgcc cgtcatttcg atgcgttctt gaggaagcac
     9541 gcggagcaga aagatacgag atacgacgat ctggccgaat gggttcgcgg cgaaaggaaa
     9601 aaactcggct cgggcgagtc cgtcgggaag aaaccggatt ccactgaaat ggaggtcaaa
     9661 tcggatttgc tcacggcgga gaaaaagtat tccgatgcag agaagatcat cagcgaggcc
     9721 attgttctcg agccgagcaa cgttcgattg tacttcaaga ggatgagcat ccggttccga
     9781 gaggcgaacc gggtcgactc ggattacagg aaggcaaaag ctaaggcaga tgaggacaag
     9841 ggaagaatca ccgtcctcga gaaaccagct tcgacaagcc aggccaaaac tcccgcgagt
     9901 gagcagctcg aggaaaacaa agcaaagcaa accccagaag aacaggagaa ggccaggcaa
     9961 gaggagctga agacgctgaa ggcggatgag gccaaacaac ggaaaacggc cgacagtgca
    10021 aaacagaagg agcggcaact ctatcaaggg gtgatcaagg attgtgagac cgttctcaaa
    10081 ctgcgcaaag acactccgaa cgcctatttt tatcgtgccg tggcgaattt ccgccttgag
    10141 aaagcccagg actcgatcat tcaggatctc aaacacgcga tcgatcttga tcccacgcat
    10201 ctagccgcta tggattggct cgctgggccc tcgatcaaga cacctacgga agaagcagcc
    10261 agattgagat tgcgatacca gcaactcttt ccaggctctg actggaacct ctggcagctc
    10321 gctgagctgc agatcaaaat gaagcagttc gcgaacgcat acgacacgat ccagaaagcc
    10381 atcgccatcg agccggtcga ttacgcatat taccaaacac gcgcggatgc cgagaaaggc
    10441 ctgggataca gcgaagcgca cattcagcgc gatcgagccg agggcaatcg ccttgccgtg
    10501 gagttcctgc gccgaaaggg gaacaaagag gatctcgaca aggcgaacga ctgggaacaa
    10561 gacgagtgga aaatcctctc cgaactcggt gacaaatcgg cgagcggtga agtgcgctgc
    10621 aattcggatg tgacgacctg ccttagtctc caggtgaccc acggttatgg cgagtgggcg
    10681 atttttggaa tcacctccgt ccaaatggcg gatcaggaca cccgtatcgt gcagatcaag
    10741 caaggaggga atgacggcat cgtgctgggc tccgcgggca gtgttttttc gctctattcg
    10801 aacgaaaagg aacatgagcg caccgtggca aacatcggca gagccgaggt tttgtccgtc
    10861 gagccgcaat ccgcgctgct cagggtaaaa atgccgaagc cggagggtga cggaatggtc
    10921 cgcgccggag atggcgttca actgctcgtc cgaacgccca aaatagaaca tcgctcgaag
    10981 ctgtggtccg tggtgaagtt caacatctcc ctcgaagatc tgaacgggaa gaagattgcc
    11041 ggcttcgaca cgctttacag caaggagtcg cctgagctgg accgggcgat ttaccagaaa
    11101 ctgctcgacg acattcagga ggtcggaaga cagttcggcg acaagtggga tgagggcaag
    11161 cccatctcga agggaaagtt tgccggtaag cccacgaggt tgcgtcaggc tctggaaaag
    11221 acctctcgag aagacttgga cggcttcttc cagtacgtcg tgaaattccc cgccacgtac
    11281 tttgggcaca attggaggat cggggggatc tatgcctatt gggtcctgtt aggcatgccg
    11341 gagaaatgat ctgagcgcaa tttctcattc ctagcgcagc gcttccatcg cctccagtat
    11401 tgccgttctt gccgcgcgac cttgactccg ccgtgtaatc gtgccacgct aacaccagtc
    11461 aatcccttcc caggaactga acttcctgca gaaaacccgt gctttgttct tgctgcttcc
    11521 gcgcggcatg gcgacaccgt gaccacacgc gagcggccgc aagaagtgta ggaaccggag
    11581 gagtccctgc cggcggaagg gagtggacaa catgcagagg cgtgccctga ttgtggtgga
    11641 tgagccggcg acgtccatgc tgatcgaaaa ggctctgcaa tcggtcggca tggaggctct
    11701 gacggcccct cacagcgcag tggcggcggc ttctctccgc gaaggaaggt tcgatctcgt
    11761 cttcctggac tctcacatga cgccgggtga tggaatcgag cttgtgcggc gttttcgcag
    11821 ttcacagctt cacttaacga ctcccattgt cctgatcagc gacgacaaga agccgggtgc
    11881 tatggcgaag ggattcgagg ccggggccag ttttttcctt tacaagccgg ttgaaaaacg
    11941 aaatgtgctc cagttggtcc gggccacgca gggaaccatc gaacacgaga ggcgtcggac
    12001 gcgacgcgtc cccgttcact cgaaggtgca cctgaggaac ggacagggtg aactggaagg
    12061 ggaaaccgtt aacatgagca tgaatggtat cctcgtgcga gcgcctttca cctttccggt
    12121 ctggtcatct ctggacgtcc ggctgtatct ctcgccgagt gctcggccca tcatcggcgc
    12181 cggtcgtgtc gcgcgaatct cgggcccaaa ccagatgggg attcatctcg gccaattgag
    12241 ttctgccgag agccagagat tgcaggagtt cctgttgcct ctgattccgg cagattagtc
    12301 cccctaccag ctcaaatcgg ggacaagcaa aatgcctcgg tggccgccac cacattcgcg
    12361 aagcttgtgg ttttgcatcc actcactcgg tgcggggaaa tggcgggggc gacgagacga
    12421 gtttatccag agcttgccga aggaaactcg cgaatcccgt gaaagcgatt cgtgttcctc
    12481 acggtggcgg gaaaatggcg gggacgacga gactcgaact cgcgacctct gccgtgacag
    12541 ggcagcgttc taaccagctg aactacgtcc ccgcggtcgg aatactcacg gatggtgggc
    12601 agtgcaggac tcgaacctgc gacctcctgc ttgtaaggca ggcgctctaa ccaactgagc
    12661 taaccgccct catctccttc atcgtacagg atgccgtaga aacggtcaac gcgctctcgc
    12721 gcatcgaagt tccgtcctcc gcctttcttg cactttcggc gccgtgccat taaactttgt
    12781 tccttgtatt tttcgtcgga gagcggatcg catgcttctt tctcgtgatt ttgtctctta
    12841 tatctccgtg gagctggtca agcgcatgat tgcggcggag atcatcgaga cgaaagccgc
    12901 cgaccaggtt acccagcgca tccgtttgcg catgcaggag gagctcggcg tcgaggaccg
    12961 tctcagcgag gaggtccgcc agattctctt ggagcatcag gatgagatgc ggcgcaccgg
    13021 cgcttcctac caggagatgt acaaaaaggt caaggcgcag ctggcccgtg accgcaagct
    13081 tatcctgcgc tagtggcaca gacttgagtc tgtgctcttt ggttggtgca gcagacttct
    13141 cgacgcatca cgttgcgttg agcgagggga accgcataaa cgaatgcgac tcagccgcga
    13201 aaagatcgtc cgcctttcgc accagatgat cgacgtgctc gtctccagcg acgacgtcga
    13261 attcatcgaa gaccgcgaaa ccatccgaaa tcagatcgtc caaatccttc aggcgcttct
    13321 gcgcgaggaa gaaaagctca acgccgatgt ccgcaagaag atcgcgtccc agaaaaaaga
    13381 aatcctcgaa ggcagcgagg agtgggattt gctttaccgc aagtattatg ccgaagagat
    13441 gcgccgcatg ggcgttccgc acctgtccga agagcgccgc gcctgaaaac agcaagggcg
    13501 gctttggcgg ccgccctttc ctccaaatca gtcctcgctc agccttctca accgcatcag
    13561 ggtgaatccg gccggcgttt cagagtcggc cggtcgtcgt ccgttccggt gtcggtagtc
    13621 tgcggtttct tcttgtcctt ttcggtccgg ctgcgcagtg ttggtttgtt cgggtcgttc
    13681 tccagcacct ttcgggtgaa catgattccc cgcaggcgcg ccttcacctg gtcgaattcc
    13741 gacgttgtca cgatgtactc atcgcgagcg ggcaagatcc gagcaatttc cttctgcgcg
    13801 ttcgcaatcc ggtccggagt gggcgggtgc gacgagaaca ccttcgccac ggtcccgggc
    13861 tggcggcgct cgtcggcctg cacgcgctcg aagaacgtca cgtaggaatt cgggtcgtag
    13921 ccggccttgt acatgtactg cagtccgaga aagtccgcct cgcgttcgtc attgcgcgag
    13981 aacttcagat aggtgagcgg aatcgccagg ttcaagccct gatagatggc atatcccgcc
    14041 cagccgccgg gccccagcag gatcagcgga atcgtcgcca attgcatgat ttggcctttg
    14101 gtggcgttct tcgttccgtg cctcgccgtt acatgcgaaa tctcatgggc catcaccccg
    14161 gccagctcgg attcctcctg cgcgcgcagg accagcccgc tgttcacgta aaagaagccg
    14221 ccgggaaggg cgaatgcatt caccacgtcc gaatcaatca ccttgatcgt gaacggcact
    14281 tgcgcatccg agttccgcac caggttttgt ccgacgcggt tgacgtactc cgttaccacc
    14341 gggtcgtcaa tcaacttcgc cgaccgctcc acctcctgcg ccagttgctt gccgatcgca
    14401 atctctctct ccagagagta gaaattgatg cccttcccga cgttgcggtt cccgatcgca
    14461 tccacatctt ccttggaatt cttcgggttg acttgcgtct gctgcgggac gggttgctgt
    14521 tgcggctgct gcgtctgcgg ctggctcgga gccggtggct gcgggctctg ggcaactccg
    14581 atcgttggcc aaccgagcag cgcgacgact aaagttaagc tcagaatcgg ccggatggat
    14641 ttccccataa ctctcacctc cacctgccgg agattataca cccctcccgg ctggacagac
    14701 ctgcacttcc tatgatgtac cggcctggcc gaaggtttcc ccgcctccgc atgtgcccaa
    14761 agagcaccgc ggccgggttg acaccctcac cgcatctctc taaagtcgaa atcaggccct
    14821 atgagcgctc gcaaggtgca gatcggccag ttgtggaaga gagaagcgac cggggagacc
    14881 tatctcgtca cgcgcgtcta ctccgaagcc ctttccacga tggtcgttct tcgcaagagc
    14941 ggcgccgagc aggaagccat catccgcgtc cgcgtcgagc gccttggcga cggccagacg
    15001 attccgggct tctctcccgc ccaggaggac gaaaagttct agtgccccgg cccgcccggc
    15061 ccctagatcg accgcaccgg catctccacg atgtcttcca cctgcatcaa ctcctttgtg
    15121 aggaacggct cgccgtattt cacgccgatc atcagcccgt agcgcctggc cagcaggctc
    15181 atatcctctt ccagccgctc aatggccagg tgcacctcgc ccctgcgcgc cttccctgag
    15241 ggaaggaact gcgatgcgaa cgccagaatc gcccggtgcc gtacctcgaa ctccgccgta
    15301 atgtccacca caaacgtcgg ccggacctcg gtaaacgccg tcgagtagag gatcttgaac
    15361 ggccggaagg cctctcccgc aatgggcaat tgcttcagcc ccgccaggaa gcaggcctca
    15421 tagcccagcg tcgaagcatg gtagtggtcc gggtgccgcg cttcccagta gggcaggatc
    15481 accgtgcgcg ggcgcaactc gcgcaaagtc tgcgcaaggc gcagcttgtg ctggcggctc
    15541 ggctgcacat ccgaatccgg cattcccagg ttaccccgcc acttcaccct gaggagcttc
    15601 gctgcacgcg ccgcttcttt cgcgcgggtt tccggtgtgc ctcgtgtccc catctccccg
    15661 gccgtgaggt ccagaatccc tgtcttgtag cccctcttgg ccattttcag cagcgtgccg
    15721 ccgcaggtca gctccacgtc gtccgggtgc gccgcaatcg ccagcaagtc cagtttcatt
    15781 gttcctccgt cccaaaactg attctaggcg cgcacccctg cctcgtcaaa gcgcgcctgt
    15841 gctagtcttt ctccacgaat cgccatgccg gatgctccgt caaccattcc cgtatctttg
    15901 caaggccgcg tcgccctggt taccggcgcc ggccggcgca tcggccgcgc cgttgccctc
    15961 cgcctcgccc gcgaaggagc cgacgtcgtc gtccactacc gcggctccaa ggccgaagcc
    16021 gcgcaagttg tctccgaaat cgaagccctt ggccgccgcg ccgtggccct ctccgccgat
    16081 ctccgcgagg tcaaatccat ccaccgcctc ttcagcgaag ccgcgcgcca gttcggccgc
    16141 ctggacatcc tcgtcaacaa cgccgccgtc ttctttcccg cgcgcgtcga aaacgccacc
    16201 gaggaacagt ggaacgccgc cctcgacacg aacttgaaag cacagttctt ctgcgcccag
    16261 gccgctgcgc cggcgctcaa ggaatcccgc ggcgtcatcg tcaatttcgc ttccatcggc
    16321 gggctgctcc cctggccggg ttacatcccc tattgcgttt cgaaagcagg cgtcatcatg
    16381 ctcacgcgat gcctcgcccg cgctctggcc ccggaggtcc gcgtcaacgc cgtcgccccg
    16441 ggcaccatct ccatgcccgg cgacccgccc gaatggcagg ccgactacat ccgtctggcc
    16501 ccgctgaaac gcaccggcac tcccgacgac atcgccgatg cggtctcctt cctcatccac
    16561 tccaccttca tgaccgggca ggtcctcgtc ctcgacgccg gccgaactct gtagcggcga
    16621 cttatgagtt ggcaacttcg tacttggtag cacaggcttc agcctgtgtc tttttctccc
    16681 gtaagccgac gcttacttcc gactgcgcga ccactcgccc ggaatcattc gttcccctgc
    16741 gcagcgacga actgcgaaat ccgggttgcc acacacacac gagtggcgct ctacgtccaa
    16801 tggctctgct ttgctcaatt ttctcggctt gatccgcgcc cattgacttc aacttctaag
    16861 cgcccctaac atgtcggtcc gaggacgaga ccctcttcac ttgattgggc agaccatctc
    16921 gcactaccgc atcgttgaaa aactcggtgg cgggggaatg ggtgtggttt accgggccga
    16981 ggacctcaag ctcgggcgct ttgtggccct gaaattcctg ccggaggagg tcgcgaaaga
    17041 cgcgcaagcc ctcagtcgct ttcagcgcga agccaaagcc gcttccgcgc tgaatcatcc
    17101 caacatctgc acgatctacg aaatcgatga tcagcatggc caagcgttca tcgccatgga
    17161 gtttctggac ggcgcaaccc tgaagcacca catcgccggc cgtcccctgg aactcgacac
    17221 cctgctggcg gtcagcattg agatcgctga cgccctggat gcggcccacg tgcaaggaat
    17281 catccaccgc gacatcaagc cggccaatat tttcgtgacc aagcgagagc acgccaaaat
    17341 tctcgatttc ggcctggcca agctcactgc ggaaaaggtt acgcccctgg agct
//
LOCUS       DASYVO010000116         6192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_142775, whole genome shotgun sequence.
ACCESSION   DASYVO010000116 DASYVO010000000
VERSION     DASYVO010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142775"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            497..1063
                     /locus_tag="VGR03_03900"
     CDS             497..1063
                     /locus_tag="VGR03_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993453.1"
                     /translation="MNSKSTNSSSTIEIPAVTTKKTSAPPRASREGRCQHRFSNGKRC
                     RKFATASHFGLCLQHFTASAAVGAGLQQPANDSLDLSADLLPELSEFDSAVDINQFLA
                     RLLVLVTQGRISPRRASVLAYITNQLLHSHRAIDRENLLQLEDANPPRVETGTIPRPG
                     QRPPDRHDGPTVIWDVPGVNLDREKAPS"
     gene            complement(1140..2753)
                     /locus_tag="VGR03_03905"
     CDS             complement(1140..2753)
                     /locus_tag="VGR03_03905"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF010030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV1993454.1"
                     /translation="MQKTLLPIQAVARKLEIPEKYFEPLGSQSGKIKLELLSDPAFKR
                     RGKLIIVTATTPTAAGEGKTVTSISLTQGLAHIGKKVIITSREPSLGPVFGMKGGAAG
                     GGLSQIEPSAKINLHFHGDFHAIGSAHNLLAALIDAHLFHGNDRQLDPDRLSWPRTID
                     MNDRALRRIAVSLDSKKEGSGRETGFIITAASEIMAIVALAKDRKDLRKRLDSIVIGV
                     TRDGKPVSARDLGATGGMMALLAEAILPNLVQTTDGTPAFVHAGPFGNIAHGTSSVTS
                     QEMGLRLADYVVNECGFASDLGAEKYFDIVMQQSGITPAAAVLVTTVRSIRNQGEGEF
                     ERGLPNLGKHIEILQTFGVPTIAAINRFPTDTDAELNRLTAYCAEHGVVSALSEGFAK
                     GGPGAKELAEKVVAAIAKNSAPTARPLYSLDEPFTEKIQKVAKKIYGASDVNFSEQAK
                     AKLQQFTEWGYGKLPVCIAKTQYSLSDDPKRLGAPTGWTLNVTNASLSAGAGFVVVIS
                     GNMMLMPGLPKVSRASSIDVDDSGAILGV"
     gene            complement(2981..4045)
                     /locus_tag="VGR03_03910"
     CDS             complement(2981..4045)
                     /locus_tag="VGR03_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEV1993455.1"
                     /translation="MKRSIFVLFFFVAAPLFAQQPPEIRFHSVPDFLKLPPDLPLGEV
                     AGVAVNSHGHIFVFSRGNSTGPAYGASAAQLLQFTPDGKFIREIGHNLYAWSFAHTVK
                     VDKDDNIWVTDKGSDMVIKFNPEGRVVMVFGRKQEASDEGTEPLKHVKPPLPPVDGMF
                     RQVTDVAWDASGNIYISDGYVNSRIAKIDKDGNWLKSWGEPGDKPGQLNTPHSIAVDA
                     QGNVYVADRGNRRIQVFDGEGKFLRQITIDVLADPNARPAIGNKPTATTGTMAPGAPW
                     AICITPGPNQVLFSSDGFPGRIYKLSLDGKVLGVFGKSGKQLGQFGWVHEIACPSENE
                     LYVAEPLNWRVQKLILEPTH"
     gene            complement(4095..4481)
                     /locus_tag="VGR03_03915"
     CDS             complement(4095..4481)
                     /locus_tag="VGR03_03915"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEV1993456.1"
                     /translation="MSSPYLLKTEPSEYSFADLQRDKVTVWDGVSNPTAVKNLREMTP
                     GARLIIYETGDRRTAVGTASAVSVDASDPKNPAVKIKVGKALAKPISLVQIKANKLFK
                     DSLLVRIGRLSVVPLTEAQFKFLTGD"
     gene            complement(4514..5029)
                     /locus_tag="VGR03_03920"
     CDS             complement(4514..5029)
                     /locus_tag="VGR03_03920"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1993457.1"
                     /translation="MTKLQTSSKQSRTSRTDSLFNRADKLEDSGNLKAAFRLFLSAAK
                     AGDLGCQLNVGNYYCGGKGIRRNLSRGLYWYKRAYRRGEASAASNIGMTWRNEGKPKK
                     ALSWFRRAVKIGDDEANLEIAKYYIENENNPRKAIPHLEKVCRSNWVTKAGAEEAAKL
                     LKQARKKLKRS"
     gene            complement(5120..>6192)
                     /locus_tag="VGR03_03925"
     CDS             complement(5120..>6192)
                     /locus_tag="VGR03_03925"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HEV1993458.1"
                     /translation="EAVARRVKEGITLMLPTEDSIWVGEDLQRRFGLPYWQFTLTATD
                     ANRFAIRIAREITQRTKILIFHYCYHGTVDESFATLENGVVGPRRGNIGPPVNPAETT
                     RVVEFNDLNALEDALKHHDVACILAEPAMTNVGIILPDDGYWPAARELARRYGSLLIA
                     DETHTICAGPGGCTNEWKLDPDVLVFGKAIGSGIPGATFGVSEEVAQRISARIHLEDC
                     DVGGIGGTLAGNALSLAAMRATLEDILTPTAFERMLPLAKRFTDGVAEHIRAAGLPWN
                     VIRLGARAEYTFAAKPPRNGGESAAAADFELERFLHLYALNRGVLLTPFHNMALISPA
                     TTAADIDAHTKIFASAAQELTA"
BASE COUNT         1212 a         1669 c         1900 g         1411 t
ORIGIN      
        1 gccctatact ccggcctctt ctcctcctaa tttccagttt ctagtttctg atttctagtt
       61 tccatcttca cttccccccc gcccctaacc cctgccctgc caatcacttc caaacttgct
      121 tcaactttat cttgacaggt acggtcctta tatgctatca tgggttgtga ttgatcgacc
      181 gcacccccgc caagcccgta agtcctgcct aatccgcgcc ttgccccttc cgtgttccct
      241 ctttaactcc caatcttcag cttctaacct ccttctttta aatgtttcga cgttcagacc
      301 ttcagacctg ccaacgttcc ccattctccg cccttccgcc tcttcaccag tcacgagtca
      361 ccagtcacta ttcacgaaat ttcccgtcgt ccatcccctt tccgttcagc aacttacaaa
      421 atgctcttcc cgcaacccct ttgttttgaa aacgatccat tttgatgggg gggtgtaccc
      481 cctccacgag gaaatcatga actcaaaatc gacgaactcc agctccacca ttgaaattcc
      541 agcagttaca acaaaaaaga cctccgcgcc tccccgggca tcccgagagg gccgttgcca
      601 gcaccgtttc tccaacggca aacgctgccg caagttcgcc accgcttccc attttgggct
      661 ttgtctccaa cacttcacgg cgagtgccgc cgtcggcgcc ggacttcagc aacccgcaaa
      721 tgattccctc gacctctccg ccgaccttct cccggaactc tccgagttcg actccgccgt
      781 cgacatcaac caatttctcg cccgcctcct cgttctcgtc acccaaggcc gcatctctcc
      841 gcgccgcgcc tccgtcctgg cctacatcac caaccagctc ctccactccc accgcgccat
      901 cgaccgcgaa aaccttctcc aactcgagga tgccaaccct ccccgcgtcg agaccggcac
      961 catcccccgc ccgggccaac ggccgcccga ccggcacgac ggccccaccg tcatctggga
     1021 tgtccccggc gtaaatctcg accgcgagaa agcaccgtca tgaccatttt ctttgagcat
     1081 cgcggcggac ctgccgctct gaatggaaga ggattgaaca acatccaaag aactgctctt
     1141 cagacgccga ggatggcgcc agagtcgtcc acgtcgatgg aggaggctcg cgaaacttta
     1201 ggaaggcccg gcatcagcat catattgccg gagatgacca cgacgaatcc ggctcccgcg
     1261 gaaagcgagg cgttggtgac gttgagcgtc catccggtgg gagcgccaag gcgcttggga
     1321 tcatcggaca aggaatattg cgtcttggcg atgcacacgg gcaattttcc gtagccccat
     1381 tccgtgaatt gctgcagttt cgctttggct tgttcgctga aattcacgtc gctcgcgcca
     1441 taaattttct tcgcgacttt ctgaatcttt tctgtgaatg gttcgtccag tgaatagagg
     1501 ggacgcgcgg taggcgcgga attcttcgcg atggctgcaa caactttctc ggcaagttct
     1561 ttcgcgccgg ggccgccttt cgcgaatcct tcggagagag cgctgaccac tccgtgttcc
     1621 gcgcagtaag cggtgagccg atttaattcg gcgtccgtgt ccgtgggaaa acggttgatc
     1681 gcggcgatcg tcggcactcc gaatgtttgc aggatttcga tgtgcttacc gaggttgggc
     1741 agcccgcgct cgaattcgcc ctcgccctgg ttgcgaatgc ttcgcaccgt ggtaacaagg
     1801 acggcggccg ccggcgtgat tcctgattgc tgcatgacga tgtcgaaata tttctccgcg
     1861 ccgagatcgg aagcaaaacc gcattcattc acgacgtagt cggcgagtcg taaccccatt
     1921 tcctgggaag tgacgctgct cgtgccatgg gcgatattgc cgaaaggtcc ggcgtgaaca
     1981 aaggcgggag taccgtcggt ggtttgcacg agattgggaa gaatagcttc cgcgagcaag
     2041 gccatcatgc cgccggtggc gcctaggtcg cgagcgctga ctggttttcc gtcgcgagtg
     2101 acgccgatga cgatggaatc gaggcgtttg cgcaaatctt tgcggtcttt cgccagcgcg
     2161 acgatggcca tgatttccga agcggcggtg atgatgaagc cggtctcgcg gccgctgcct
     2221 tcctttttgc tgtcgagcga aacggcgata cggcgcaggg cgcggtcatt catgtcgatg
     2281 gtgcgcggcc agctgaggcg gtcgggatcg agctgccgat cgttgccgtg gaaaagatgt
     2341 gcgtcgatca gcgccgctaa caaattgtgt gcagagccaa tggcgtgaaa atcgccgtga
     2401 aagtggagat tgattttcgc gctcggttcg atctgcgaaa ggccgccacc tgcagcaccg
     2461 cctttcatgc cgaacaccgg gccgagcgac ggctcgcgcg atgtaattat cactttcttg
     2521 ccaatgtgcg cgaggccctg cgtcaagctt atggaagtga cggtcttccc ttctcccgcg
     2581 gctgtgggcg tggttgcggt gacgatgatt aatttgccgc ggcgcttgaa ggcgggatcg
     2641 gagagcagct ccagtttgat ttttccgctt tgtgatccca atggctcaaa atatttttcg
     2701 ggaatctcga gtttgcgtgc taccgcttga atcggaagaa gcgtcttttg cataagagtt
     2761 atgaattttg ccagtaaaaa ttccgcgcgc ggagaaaaag gataacacat ttgccgcgct
     2821 tgccattcac ttgatgatcg tcaaggcagc cggtggtgaa aagtgcgagt gttctttggt
     2881 tcggaagaaa gaatttaaca cagagttcac agagagcaca gaggacacgg agcggagaga
     2941 aaccgcggaa agtatttcaa ggtatttatg ggacgcagcg ctaatgagtt ggttcgagaa
     3001 tgagtttttg gacgcgccag ttcaaaggtt cggcgacgta aagttcgttt tcggaggggc
     3061 aggcaatttc gtgaacccag ccgaattgcc cgagttgctt gccggacttg ccgaagacac
     3121 cgagaacttt tccgtcgagg ctgagtttgt agatgcggcc gggaaaacca tcggaactga
     3181 agaggacttg attggggccg ggcgtgatgc aaatagccca aggcgcgccg ggggccatgg
     3241 tgccggtggt ggcagtgggc ttgttgccga tggcggggcg ggcgttggga tcggcgagaa
     3301 cgtcgatggt gatctggcgg aggaattttc cttcgccgtc aaagacctga atgcggcggt
     3361 tgccgcgatc ggcgacgtag acattgcctt gcgcgtcgac ggcgatgcta tgcggggtgt
     3421 tgagctggcc gggcttatcg ccgggttcgc cccacgactt gagccagttg ccgtctttgt
     3481 cgattttggc gatgcgggaa ttgacgtagc catcgctgat gtagatgttg ccggaagcgt
     3541 cccacgcgac gtcggtgact tggcggaaca tgccgtcgac aggcggcagc ggcggcttga
     3601 cgtgcttgag gggctcggta ccttcgtcgg aggcttcctg cttgcggccg aagaccatga
     3661 caacgcggcc ttcgggattg aacttgatga ccatgtcgga acctttgtcg gtgacccaga
     3721 tgttgtcatc cttgtcgacc ttgacggtgt gcgcgaagga ccaagcgtag agattgtggc
     3781 cgatttcgcg gatgaatttt ccatctgggg tgaactggag aagttgcgcg gcgctcgctc
     3841 cataggcggg accggtgctg ttgccacgcg agaagacaaa gatgtgccca tgcgaattga
     3901 ccgcgacgcc ggcgacttcg ccgagaggga ggtcgggcgg aagtttcaga aagtcgggga
     3961 cggagtggaa acggatttcg ggcggttgct gggcgaaaag aggagcggcc acgaaaaaga
     4021 acaaaacgaa gatggagcgt ttcatgtgat tcagcctcgc gacttcgccc cgcatcatac
     4081 gcgggttcgg gaaatcagtc gcctgttaga aatttgaatt gggcttcggt gagaggaact
     4141 acggagaggc ggccgattcg gacgagtagg gaatccttaa agagtttgtt ggctttgatt
     4201 tggaccagcg atatcggctt tgcgagcgct tttccgactt tgatcttgac ggcgggattt
     4261 ttgggatcgg aggcgtccac ggagacggca gaagcggtac cgacggcggt gcggcggtcg
     4321 ccggtttcgt agatgatgag cctcgcgccg ggcgtcatct cgcgaagatt ctttacggcc
     4381 gtcggattcg agacgccgtc ccatacggtc accttgtcgc gctgaagatc ggcgaaggag
     4441 tattcggaag gttcggtttt gagcaagtag ggggaagaca tcagcgccgc ctttattgtc
     4501 agaggattcc catctaggat cgcttcaatt tctttctagc ttgcttgagc aactttgcag
     4561 cttcctcggc tccagccttg gtgacccaat ttgaccggca cactttttcc aaatggggaa
     4621 tggccttacg cggattattt tcattctcga tgtagtattt ggcgatttcc agattcgcct
     4681 cgtcatcccc aattttgact gcccgccgaa accatgagag cgcctttttc ggcttccctt
     4741 cgtttcgcca ggtcattcct atgttactgg ccgcactcgc ttctccgcgc cgatatgccc
     4801 gcttatacca atagagaccc cgtgacaggt tgcgccgaat gccctttccg ccgcagtagt
     4861 aatttccaac gtttagttgg caacctaaat ctccagcctt ggcagcggaa aggaaaagtc
     4921 gaaaggctgc tttcagattg ccgctgtctt caagtttgtc ggcacgattg aaaagagaat
     4981 cggtcctcga tgttcgagat tgcttggaac tagtttgtag ttttgtcatg gagctttgcc
     5041 gacttcgctt tgtcggatgc ctccttattc tacatcggaa ataaatggta gacgagacaa
     5101 acggaaaggg tgagggcagt tacgcagtga gttcttgggc ggcagaggcg aagattttgg
     5161 tgtgggcgtc gatgtcggcg gcggtggtgg cgggggagat gagggccatg ttgtgaaaag
     5221 gcgtaagcag gacgccgcgg ttgagggcgt agaggtggag gaagcgttcg agttcgaaat
     5281 cggcggccgc ggcggattcg ccgccgttgc gcggaggttt tgcggcgaag gtgtattcgg
     5341 cgcgagcgcc gaggcgaatg acgttccagg ggaggccggc ggctcgaata tgttcggcga
     5401 cgccatcggt gaagcgcttg gcgagcggga gcatgcgttc gaatgcggtg ggcgtgagga
     5461 tgtcttcgag cgtggcgcgc atggccgcta gcgagagggc gttaccggcg agcgtgccgc
     5521 caatgccacc gacgtcgcag tcttcgagat ggatgcgggc ggagatgcgc tgggcgactt
     5581 cttcggagac gccgaaagtg gcgccgggga tgccgctgcc gatggctttg ccgaagacga
     5641 gcacgtcggg atcgagtttc cattcattgg tgcagccgcc ggggccggcg cagatggtgt
     5701 gggtttcatc ggcgatgagg agtgaaccgt agcggcgcgc gagttcgcgg gcggcgggcc
     5761 agtagccgtc gtcgggaaga atgatgccaa cgttggtcat ggcgggctcg gcgaggatgc
     5821 aggcaacgtc gtgatgcttg agggcgtctt cgagagcgtt gaggtcgttg aattcgacga
     5881 cgcgagtggt ttcggcggga ttgacgggcg ggccgatatt gccgcgacgc gggccgacga
     5941 ctccgttttc aagcgtggcg aaggattcgt caaccgtgcc gtgatagcaa tagtggaaaa
     6001 tgagaatttt tgtgcgctgg gtgatttcgc gggcgatgcg aatggcgaag cggttggcgt
     6061 cggtggcggt gagtgtgaat tgccagtacg gaaggccgaa gcggcgctgg agatcttcgc
     6121 cgacccaaat ggaatcttcg gtggggagca tgagggtgat gccttctttg acgcggcggg
     6181 cgacggcttc gg
//
LOCUS       DASYVO010000117         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_17160, whole genome shotgun sequence.
ACCESSION   DASYVO010000117 DASYVO010000000
VERSION     DASYVO010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17160"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /gene="ispE"
                     /locus_tag="VGR03_03930"
     CDS             complement(<1..822)
                     /gene="ispE"
                     /locus_tag="VGR03_03930"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEV1993459.1"
                     /translation="MPEVRIPAFAKINLRLDILGKRADGFHELRTIFQTISLHDELRL
                     RASRRPGIALSIEGNQPLSAEPVEENLVYRAVDALRRELRIRGGVEIELKKTIPAGRG
                     LGGGSSDAAAALLGYLRLMKKKLAAARLMEIAASLGADVPFFLWGGRALGVNKGDEIY
                     PLPDIRKLYFLVVSPKEIHVPTPVAYRWLNARAPRLTKSGGNPKLWEFCALCWSAQGS
                     GLSNDFEGPVFRRHRRLDQIKRELLQRGAAEASLAGSGSAVFGVFPSPAMARRAAV"
     gene            complement(831..>2384)
                     /locus_tag="VGR03_03935"
     CDS             complement(831..>2384)
                     /locus_tag="VGR03_03935"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1993460.1"
                     /translation="LYRLKNEHDKAEQVLKSILKTDPENEPAVEQLTQLLMDEGKSPE
                     AVTLLEGITAHSPSPVLLDLLGDAHTQAHELAKAEAAYRKAVELDPSELSHQRGLGQT
                     LLAEEKYPEALKVYQKLSDVMPDDSDVYLRIAQIYRELHQLDKAEENLVKARQYAPGS
                     LEVMYNEAMLYQAQGRFEDAIRVLSNAVTDIKGQSPTQPSRRRSLAILYQQLGQLYRD
                     TQNYQASIYTFEELGHLGEEEDRRARMMIMDTYRAAKDLPKALQTGKEALAKYPADPA
                     IRTSYALLLGENGQTDEAVKMLRAQLHGDAGDRETYLNVAQVYERGRRYKEAEEAARA
                     AEVLPGQARENEMVWFLLGAIYERQKFFDKAEEQFKKVLAVNPKNAPVLNYYGYMLGD
                     LGIRLDEAEALVQQALKEDPFNGAYLDSLGWIYFKENKLSASEITLRKAVERERHDAT
                     IHAHLGDLYAKTGRGDLAAAEWEKSLVEWRRALPADMEVGKVAELEKKISQSKHRVAQ
                     KSTASEAKP"
BASE COUNT          439 a          791 c          619 g          535 t
ORIGIN      
        1 aacagcggct cggcgcgcca tcgctgggct cgggaaaact ccaaacaccg ccgaaccgct
       61 acccgccagc gaggcttctg cagctcctct ttggagcaac tcccgcttga tttgatcaag
      121 ccggcgatgc cgccggaaaa ccggcccctc gaaatcgttc gaaaggccgc ttccctgcgc
      181 gctccagcat agagcacaga actcccaaag tttagggttt ccgcctgatt ttgtcaatct
      241 cggagccctg gcgttcagcc aacgataggc gactggcgtc gggacatgaa tctccttcgg
      301 cgaaaccacg agaaagtaca gcttacgaat gtccggcagg ggatagatct catcgccctt
      361 attcactccc agcgcccgcc cgccccatag aaaaaacggc acatccgcgc ccagcgacgc
      421 ggcaatttcc atcaaacgcg cagccgccaa tttcttcttc atcaaccgca gataccccag
      481 aagcgccgcg gcggcgtcac tcgatccccc gcccaggcct cggcccgctg gaatcgtctt
      541 cttcaattcg atttccaccc cgccgcggat tcgcagttca cgccgcaacg catccacggc
      601 gcgatagaca agattttcct ccactggttc cgcggagagt ggctggttcc cctcgatgct
      661 caatgcaatt cccggccgtc gtgacgcgcg caaccgcaat tcgtcgtgca gcgagatagt
      721 ctgaaaaata gtccgcaatt cgtgaaatcc atcggcgcgt ttcccgagga tatccaggcg
      781 gagattgatt tttgcaaacg cgggaatgcg gacttcgggc attcgctgaa ttacggcttc
      841 gcttcgctcg ccgtggattt ttgcgccacg cgatgcttcg attgcgaaat cttcttctcg
      901 agttccgcga ccttgccgac ttccatgtcc gcgggcagcg cgcgccgcca ttccaccagc
      961 gatttttccc attccgcggc ggctagatcg ccccggcccg tcttggcgta aagatcgcca
     1021 aggtgcgcat gaatcgtcgc gtcgtgcctt tcgcgctcca cggccttgcg cagcgtgatc
     1081 tccgaagcgc tcagcttgtt ctctttgaaa taaatccagc ccagactgtc gaggtatgcc
     1141 ccattgaaag ggtcttcctt tagcgcctgt tggaccagcg cttccgcttc gtcaagccgt
     1201 atacccaggt cgcccagcat gtagccgtaa taattcagca ctggagcatt tttcgggttc
     1261 acggcaagca ctttcttgaa ctgctcctcg gccttgtcga aaaacttctg ccgctcgtag
     1321 atggctccga gcaggaacca caccatttcg ttttcgcgcg cctggcccgg caaaacttcc
     1381 gcggcgcgag ccgcttcttc ggcttccttg taacgccgcc cgcgctcgta cacctgggcg
     1441 acgttcagat acgtctcgcg atctcccgca tcgccgtgga gttgcgctct caacatcttg
     1501 accgcctcgt ccgtttgtcc gttttctcca agcaacaggg catagcttgt acgaatcgcg
     1561 ggatcagccg ggtatttcgc cagcgcttcc ttgcccgtct gcagcgcttt gggcaaatct
     1621 tttgcggcgc ggtacgtgtc catgatcatc atccgcgcgc ggcgatcctc ttcttcgccc
     1681 agatgcccca attcttcgaa ggtatagatc gacgcctggt aattctgcgt atcgcgataa
     1741 agctgcccaa gctgctgata caggatggcc agcgaccgcc gccgcgaagg ctgcgtcgga
     1801 gactgcccct tgatgtccgt caccgcgttc gacaaaacgc ggatggcatc ttcgaagcgc
     1861 ccctgcgcct ggtacagcat cgcttcgttg tacatgacct ccaggctgcc cggtgcgtac
     1921 tgccgcgcct tcaccagatt ctcttctgcc ttatcgagct ggtgcagctc gcggtaaatc
     1981 tgcgcgattc gcaaatacac gtccgaatcg tccggcatca catccgaaag cttctggtac
     2041 accttcaacg cttcgggata tttttcttcg gccagcaagg tctgccccag cccgcgctgg
     2101 tggctcagct ccgaaggatc aagctcaacg gccttgcggt aggccgcttc cgccttcgcc
     2161 agctcatgcg cctgcgtgtg cgcatcgccc agaagatcca gcagcaccgg cgagggagaa
     2221 tgcgcggtga ttccttccag cagcgtgacc gcctcagggg acttgccttc atccatcagc
     2281 aactgcgtca gttgctcgac ggccggttcg ttttccggat cggtcttcag gatgctcttc
     2341 agcacctgtt cggccttgtc gtgttcgttc ttcagccggt aaag
//
LOCUS       DASYVO010000118         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_87984, whole genome shotgun sequence.
ACCESSION   DASYVO010000118 DASYVO010000000
VERSION     DASYVO010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87984"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="VGR03_03940"
     CDS             complement(<1..1299)
                     /locus_tag="VGR03_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEV1993461.1"
                     /translation="MNAAVQATIEFLRLYWAPLLCGVAVVAILPLVAGYIVLVERKLM
                     ADMQARLGPMRVGPHGLLQPIADAVKLLIKEDIIPDDADKLVFWLAPVLSVGAALLSL
                     AGLAIGPAFQIAQDINIGILFVVGISAMGIFGIVLGGWASNSNYSLMGALRSSAQLVS
                     YETAAGMALVSGLLLAGTLNIKSIVEAQDKQGIWFGMLAPVAFFIYVVASIAETNRAP
                     FDLPEAESELVAGYMTEFSGFRWSLYFLAEYTNMIVVASVATTLFLGGWLRPFPNIGW
                     LNFLDFVPPLLLMAIGAYCVLRVPKQPTRVQQLFMLAVAGLCFVVALVLAAPVILPVV
                     KPSLRAAVAPFYAGIHGGFWFIFKVSAYIYLFMWLRFTLPRYRFDQLMRLGWHFLIPL
                     SIINVLLVGVALIVGAYFHLQGWGLLLVTTPAAVIALVAAL"
     gene            complement(1299..2393)
                     /locus_tag="VGR03_03945"
     CDS             complement(1299..2393)
                     /locus_tag="VGR03_03945"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEV1993462.1"
                     /translation="MGADELVINMGPQHPSTHGVLRVKLKLDGERVIGSECIIGYLHR
                     GVEKIAENRTYQQFAPYVDRMDYVAAVSSGLGYCEAVEKLLVAEAPPRARVIRTILTE
                     LQRIASHLLWLGTHALDIGALTPLFYCFREREEILKIFEKYCGARLTTHAFRIGGLQY
                     EAYDTFEKEVERFCKDFESRIVEYETLLTQNRIWIGRTKGVGILTAADAIAFGVTGPV
                     LRASGVKWDIRKAAPYAAYDQYKFEIPVGTTGDTYDRYLVRMEEMRQSRLICLQAIEN
                     IPAGPIMAKVGKVLKPPPGEVYHAIEAPKGEIGYYIVSDGTVMPYRVHIRPPSFINLQ
                     AFDRMVRGHLVADVVAIIGTIDIVLGEVDR"
BASE COUNT          568 a          801 c          665 g          454 t
ORIGIN      
        1 caatgcggcc acaagcgcga tgacggccgc gggagtcgta accaggagca gtccccagcc
       61 ttgcaagtga aagtacgctc cgacgatcaa ggccacgcca accagcaaca cgttgatgat
      121 ggacagcggg atcaggaaat gccatcccag gcgcatcaac tgatcaaacc ggtaccgcgg
      181 cagggtgaat cgcagccaca tgaacagata gatgtaggcg ctcactttga aaataaacca
      241 gaagccgccg tgaatgcccg cataaaacgg cgccacggct gcgcggagcg acggtttgac
      301 caccggcaaa atgaccggcg ccgcgagcac cagcgcaacc acaaaacaga gacccgccac
      361 agccagcatg aacaactgct gcacgcgcgt cggctgcttt ggcacgcgca agacgcaata
      421 ggcgccgatg gccatcagca gcagcggcgg aacaaaatcc agaaaattca gccagccaat
      481 attcgggaag ggccggagcc acccgcccaa gaaaagcgtc gtcgcaacgg acgccaccac
      541 gatcatgttg gtgtattccg cgagaaaata gagcgaccag cggaatccgc tgaattccgt
      601 catgtaaccg gccacgagtt cggactcggc ttccggcaaa tcgaacggcg cgcgattcgt
      661 ctccgcaatg gaagccacaa cataaataaa aaacgccacc ggcgcaagca tcccaaacca
      721 gatgccctgc ttatcctgcg cttctacaat cgacttaatg ttcaacgtcc ccgcgagcag
      781 cagcccgctc accaaagcca ttcccgcggc agtctcgtaa ctcaccaact gcgccgaact
      841 tctcagcgcc cccatcagcg aatagttgct attcgaggcc cagccgccca gcacgatgcc
      901 gaatattccc atcgcgctga tgcccacgac gaagagaatg ccgatattga tgtcctgcgc
      961 aatctgaaat gccggtccga tcgctaagcc ggccagcgaa agcagcgccg cgccgacgga
     1021 aagcaccggt gccagccaga agacgagctt gtccgcatcg tccgggataa tgtcttcttt
     1081 gatcagcagc ttcacagcgt cggcgatcgg ttgcagcaaa ccgtgtggtc ccacgcgcat
     1141 gggccccagc cgcgcttgca tgtccgccat gagcttgcgt tccaccagca cgatgtaacc
     1201 ggcgaccagc ggcaggatgg cgacgacggc cacgccacag agcagcggcg cccagtagag
     1261 cctcaagaac tcaatcgttg cctggactgc ggcattcatc agcggtccac ttctcccagc
     1321 acgatatcaa tcgttccgat gatggccacc acatccgcga ccaagtgtcc ccgcaccatg
     1381 cggtcgaacg cctgcaaatt gatgaaggaa ggcggccgga tgtgcacacg atacggcatg
     1441 actgtgccgt cgctcacgat gtaatagccg atttcgccct tcggcgcttc aatcgcgtgg
     1501 tagacctcgc ccggcggcgg cttcagcacc ttccccactt tcgccatgat cggcccggcg
     1561 ggaatattct cgatggcttg caggcagatc agcctcgatt gccgcatctc ttccatccgg
     1621 accaggtagc gatcgtacgt gtctccggtt gtgccgacgg gaatctcaaa cttgtattga
     1681 tcgtacgccg cgtaaggcgc ggccttgcga atgtcccact tcacaccgct ggcccggagc
     1741 actggcccgg tcacgccaaa agcgatggca tccgccgcgg tcagaatccc cacgcccttg
     1801 gtacgcccaa tccagatgcg gttttgcgtg agcaaagtct cgtattccac aatgcgtgat
     1861 tcaaaatcct tgcagaaccg ttccacttcc ttctcgaacg tgtcgtaggc ctcgtattgc
     1921 agcccgccga tgcggaacgc atgcgtggtc aggcgcgccc cgcagtactt ttcaaaaatc
     1981 ttcagaattt cttcgcgttc gcggaagcag tagaagagcg gagtgagcgc gccaatgtcc
     2041 aaggcatgcg tgccgagcca caggaggtgg ctggcgattc tttgcagctc cgtcaagatg
     2101 gttcgaatca cgcgcgctcg cggcggagct tccgccacca gcaatttttc caccgcctcg
     2161 caatatccca ggccgctgct gacggccgcg acgtagtcca tgcggtcgac gtagggagca
     2221 aattgctgat acgtgcggtt ttccgcgatt ttctcgacgc cccggtgcaa atacccgata
     2281 atgcactccg agccgatcac gcgctcgcca tcgagcttaa gcttgacacg caacacgcca
     2341 tgtgtggacg gatgctgtgg ccccatgttg atgaccagct catcggcgcc caacagcgtc
     2401 ttgtgatcgt ttgtggtatc gatactcagc gtcgttttcc ctttgcggct aaaatcgcct
     2461 cactgcccgc tctcaattcc aagatttt
//
LOCUS       DASYVO010000119         4387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_257449, whole genome shotgun sequence.
ACCESSION   DASYVO010000119 DASYVO010000000
VERSION     DASYVO010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257449"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VGR03_03950"
     CDS             complement(<1..420)
                     /locus_tag="VGR03_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993463.1"
                     /translation="MRPFATALTALSLFFASASVRTDPSQEIKSMNVKRITPVLLVQE
                     IEPVVPFWVDRLGFAKTIEVPDGNRLGFVTFQKGTAEVMYQTYASVEKDAPKEVSDTA
                     RKGPTYLYMEVDDLDFVLAAMKDVKIVMPVRTAFYGMK"
     gene            508..786
                     /locus_tag="VGR03_03955"
     CDS             508..786
                     /locus_tag="VGR03_03955"
                     /inference="COORDINATES: protein motif:HMM:NF023159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEV1993464.1"
                     /translation="MIGTTRELGNLLSIGPAMLRDFELLGIRTVAQLARQNPQRMYEK
                     LGRVAGQHQDICVLDVFCAAVAQARNPRLAVEQCQWWYWSKKRKTEKK"
     gene            867..1451
                     /locus_tag="VGR03_03960"
     CDS             867..1451
                     /locus_tag="VGR03_03960"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1993465.1"
                     /translation="MALTTPDLVLLSLLAERPMHGYEADLELERREIRDWAGISRPQV
                     YYSLEKLERAGLIRSSETEEPAAGPERSSYETTAKGRAGLAAALEREEWATQRERPPF
                     VSWIALSWLARPGVFQKQMERRRTFLEEELRREKETLRGILKEVGHPYHEAVWMVSLM
                     IEQFRVELKWLKKLAQELPKRESARRPSYVLAEN"
     gene            complement(1562..2608)
                     /gene="purM"
                     /locus_tag="VGR03_03965"
     CDS             complement(1562..2608)
                     /gene="purM"
                     /locus_tag="VGR03_03965"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV1993466.1"
                     /translation="MKYADAGVNIAVADDAKQRIRHHASRTFTSGVLGGIGSFGALFA
                     LDRKKWKEPVLVSSADGVGTKLKIAMAMGVHSTVGGDLVNHCINDILVQGAEPLFFLD
                     YLAMGKLDPQVVEQLVEGMSRSCRKAGCALIGGETAEMPGFYPPGEYDLAGFIVGAVE
                     RKKLLTGKSVQPGDTLIALPSAGLHTNGYSLARKLVFDVAKLKPDTYVTEVGNKIGAE
                     LLKPHLCYAPALKNIVARGWVSALAHITGGGIPGNLPRVLPSGVKAEIDLASWPVPPI
                     FKYLAKVGKIDTDELLQSFNMGVGMILIVPPKNVKSVEINLKTHREKFFRIGRIERSE
                     SGKARVAYSGSLNL"
     gene            complement(2746..3390)
                     /gene="lepB"
                     /locus_tag="VGR03_03970"
     CDS             complement(2746..3390)
                     /gene="lepB"
                     /locus_tag="VGR03_03970"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV1993467.1"
                     /translation="MKTKIQREIIAWFWVGLAFLLINGTIGQARVIPSGSMENTLLIG
                     DHLIMSRIGYDAGVPFTNWHLPLWRNPKRQQVIIFKPPFAPDTPDYVKRVIGLPGDTL
                     DIHDGAVWINGQKLAEKYTNGTSEPYDMRPPYKVPADCYFVMGDNRGNSYDSRFWGCV
                     PRKDIIGTPVIIYMSLNVSPEAWEPGQIRERFFAYANALLHPRIVRWNRIFRTF"
     gene            complement(3425..>4387)
                     /locus_tag="VGR03_03975"
     CDS             complement(3425..>4387)
                     /locus_tag="VGR03_03975"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV1993468.1"
                     /translation="YAVRDPRGFRPLVLGKLTTPGGFQAWLVASETCAFDLLNAQYVR
                     EIEPGEMVRISKMGIESIHFAPEKPLQQCIFEHVYFSRPDSIIFGRSVNESREMLGRL
                     LAREHPVDADIVVPVPDSGVPAAVGYALESKIPFRMGLIRNHYIGRTFIEPSQAIRNF
                     GVKLKLNPIRGLIEGQRIVLVDDSIVRGTTSRKIVRMVRECGAKEVHVRISCPPTISP
                     CYYGVDTPTREELIASSNSPEEICKFLGADSLGYVSMPALRHAVSDTEGKFCTSCYTG
                     VYPTDLVQLEVREGAPNGKSPEATHGYESEPGPVERPMAVEREG"
BASE COUNT          944 a         1148 c         1413 g          882 t
ORIGIN      
        1 tttcatgcca tagaaggcgg tgcggacggg catgacgatt ttcacgtctt tcatggcggc
       61 taggacgaag tcgagatcgt ctacctccat gtagagatag gtggggcctt tgcgggcggt
      121 gtcggaaact tctttggggg cgtctttttc cacgctggcg taagtttggt acatcacttc
      181 agcagtgcct ttctgaaatg tgacaaaacc gagtctgttg ccgtcgggaa cttcgatggt
      241 tttcgcgaaa ccgaggcgat cgacccagaa ggggacgacg ggctcgattt cctggacgag
      301 caggaccggg gtgatgcgtt tgacgttcat ggatttgatc tcctgagatg gatcggtgcg
      361 aacggaagcg gaagcgaaaa acagagagag ggcggtgaga gccgtggcaa atgggcgcat
      421 accggatttt ctccttgcct tcgacagtag tctcatttag actagttaag aatagagaag
      481 gcaagaaacc aagtcaagga gatttttatg attggaacga cgcgggagct tggaaacctg
      541 ctatcgatcg gaccggcgat gctgagggat ttcgagctac tggggattcg aaccgtggcg
      601 caactggcgc ggcagaatcc gcaacgaatg tatgaaaagc tcggccgtgt ggcggggcag
      661 catcaggaca tctgcgtgct ggatgttttt tgcgcggcgg tggcgcaggc gcggaatccg
      721 cggctggccg tggagcaatg ccagtggtgg tattggagca agaaacgcaa gacggaaaag
      781 aaatgaagag acaagaaaac gcagagacgc ggagagcgca gaggagcgca gaaagaccga
      841 gaagaattgc gcgcgcgggc aggaaattgg cactgacgac gccggatctg gtgctgctga
      901 gtttgctggc ggagcggccg atgcatggct atgaggccga cctggaactc gagagacgcg
      961 agatacggga ctgggcggga atctcgcggc cgcaggtcta ctactcgctg gagaaactcg
     1021 agcgggcggg actcattcgg tcttcggaaa cagaagagcc ggcagccgga ccggagcgga
     1081 gttcttacga aacgacagcg aagggtcgcg cgggactggc agcggccctc gaacgcgaag
     1141 agtgggccac gcaacgtgaa cggccgccgt ttgtgtcgtg gattgcgctt tcgtggctgg
     1201 cgcggccggg cgtttttcaa aagcagatgg agcggcggcg aacatttctg gaagaggaac
     1261 ttcggcggga gaaggagact ctgcggggaa ttctcaaaga ggttggccat ccttaccatg
     1321 aagcagtatg gatggtgagc ctgatgatcg agcagtttcg agtggaattg aaatggctga
     1381 agaaactggc tcaggaattg ccaaagcgtg agagcgcaag gcggccgagt tatgtgctgg
     1441 cagaaaactg agagaaaaac gaatgccaaa gagttaacac agaggacgca tagagcacag
     1501 agtacacaga gaagaaaaga gcaggccctc acccctgaag gaatgagcta cagatttccg
     1561 gctagagatt gagggagccg gagtaagcga cgcgggcttt gccggattcg ctgcgctcga
     1621 tgcggccgat gcggaagaat ttttcgcggt gcgtcttcag attgatttca acggacttca
     1681 cgtttttcgg cgggacgatg agaatcatcc caacgcccat attgaaggat tgcagcaatt
     1741 cgtccgtatc tatcttcccg accttcgcga gatatttgaa gatcgggggc acgggccagg
     1801 acgccagatc aatttccgcc ttcacgccgc taggcagcac acgcggcaag tttcccggaa
     1861 tgccgccgcc ggtgatgtgc gcgagggcgg aaacccagcc gcgggcgacg atgtttttta
     1921 gagcgggcgc gtagcacaga tgaggcttca gcagctccgc gccaatttta tttccgactt
     1981 cggtgacgta ggtatccggc ttgagtttcg ccacatcgaa gaccagtttg cgcgccagtg
     2041 aatagccgtt ggtgtgcagg ccggctgacg gcagcgcgat tagagtgtct ccgggctgga
     2101 cgctcttgcc ggtcaaaagt tttttgcgtt cgacggcgcc gacgataaag ccggccagat
     2161 cgtattcgcc gggcggatag aagccgggca tctccgcggt ctctccgccg atcagggcgc
     2221 agccagcttt gcggcaggag cggctcatgc cttcgacgag ctgctcgacg acctgcggat
     2281 cgagcttgcc catggcgagg tagtcgagga aaaacagcgg ctcggcgccc tggacgagaa
     2341 tgtcgttgat gcagtgattc acgaggtcgc cgccgacggt cgagtgcaca cccatggcca
     2401 tggcgatttt gagcttggtg cccacgccgt ccgcgctgga gacgagcacg ggctctttcc
     2461 attttttgcg gtcgagcgcg aagagcgcgc cgaagctgcc gatgccaccg agcacgccgg
     2521 aagtgaacgt gcggctggca tggtggcgga ttctttgctt cgcgtcatcc gcgacggcga
     2581 tattcactcc ggcatcggca tatttcatga gagacccgtt cgcttccgct tctgatcttt
     2641 ttccgatcgg ggcgcacgct gccgcgcgcc ggacactgac aggaattcat tggagcgccg
     2701 tggagggtcg cggcggctat tgcaagaacg gctgttcgac aaaaactaaa acgtacggaa
     2761 aatccggttc cagcgcacga ttctgggatg cagcagggca ttcgcgtagg caaaaaaccg
     2821 ttcgcgaatc tgtcccggct cccaagcctc cggcgaaaca ttcagcgaca tgtaaattat
     2881 gacgggcgtt ccgatgatgt cttttcgcgg gacgcagccc cagaagcggc tgtcgtagga
     2941 atttccgcga ttgtcgccca tgacgaaata acaatccgcg ggaaccttgt acggcgggcg
     3001 catgtcgtag ggctcactcg tgccattggt gtatttttcg gcgagttttt ggccattgat
     3061 ccagacggcg ccatcgtgaa tgtcgagcgt gtctccgggc agcccgatga cgcgcttcac
     3121 atagtccggc gtatccggcg caaacggcgg cttgaagatg atcacttgct gacgcttggg
     3181 attgcgccaa agcggcaagt gccaattggt gaagggcaca ccggcgtcgt agccgatgcg
     3241 gctcatgatg aggtgatcgc cgatgagcaa cgtgttctcc atcgagccgg agggaatgac
     3301 gcgcgcctgg ccgatcgtgc cgttgatgag gagaaacgcg aggccgaccc agaaccacgc
     3361 gatgatctcg cgctgaatct tagttttcac aggcaggttt gcttcttctt gcgttttctt
     3421 tttgtcagcc ttcacgttcc accgccatcg gccgctctac gggcccgggt tcgctctcgt
     3481 aaccatgcgt cgcttcaggg gacttgccgt tcggcgcgcc ttcgcgcacc tcaagctgca
     3541 ccagatccgt cgggtaaact cccgtatagc aagacgtgca aaacttgcct tcggtgtcgc
     3601 tgaccgcatg ccgtagagct ggcatcgaaa cgtatccaag cgaatcggcg ccaaggaatt
     3661 tgcagatttc ctcgggcgaa ttcgaggaag cgatcaattc ttcgcgggtc ggcgtatcca
     3721 cgccgtagta gcacggggaa atcgtcggcg ggcaactgat gcgcacgtgg acttcttttg
     3781 cgccgcattc gcgcaccatg cgcacgattt tgcggctggt cgtgccgcgc acgatggagt
     3841 cgtcgacaag cacgatgcgc tggccttcaa ttaagccgcg gatcggattc agcttgagct
     3901 ttacgccaaa attgcggatc gcttgcgacg gctcaatgaa tgtccgtccg atgtagtggt
     3961 tgcgaatcaa tcccatgcgg aagggaatct tggactccag cgcataaccc acagccgcgg
     4021 gaacgccgga atcgggcacg ggcacaacga tgtccgcgtc gacggggtgc tctcttgcga
     4081 ggaggcgccc gagcatttcg cggctctcgt tcacggagcg gccaaaaata atgctgtccg
     4141 ggcgggagaa atagacgtgc tcgaaaatgc actgctggag tggcttttcg ggcgcgaagt
     4201 ggatcgactc gatgcccatt ttcgagatgc gcaccatttc gcccggctcg atttcacgca
     4261 cgtattgcgc attgaggagg tcgaacgcgc aggtctctga cgcgaccagc cacgcctgga
     4321 agccgccggg cgtggtcaat tttcccagca ccagcggacg gaaaccgcgc gggtcgcgca
     4381 ccgcgta
//
LOCUS       DASYVO010000120         3540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_46673, whole genome shotgun sequence.
ACCESSION   DASYVO010000120 DASYVO010000000
VERSION     DASYVO010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46673"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VGR03_03980"
     CDS             <1..835
                     /locus_tag="VGR03_03980"
                     /inference="COORDINATES: protein motif:HMM:NF025454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HEV1993469.1"
                     /translation="TIQFAAEWLENNLPALPAETPLLKPLLVATPVPAHQEEVEGPHG
                     SYAPLASYLEALPVDTASVPLTFQQIEDILKKELPRSASEYRAWWSNDPMKPQSAAWL
                     DEGWRTTSVSMTGRRLTFVRTNDREEAYISFFAKLNARLATETDFPLRSVSPQGQNWL
                     VLASLDRSRPELATIVASFAWRKRLRIELYLEGGDKDANKQLFERLLARKSEIEQVVG
                     EPLQWERLDNRRACRVAVYTRAQILTDADSPTLLEWAAKKATYFYQAFEPEFPPGRSP
                     G"
     gene            1148..1597
                     /gene="rplM"
                     /locus_tag="VGR03_03985"
     CDS             1148..1597
                     /gene="rplM"
                     /locus_tag="VGR03_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV1993470.1"
                     /translation="MRTYVAKGEEAEALKVGANWFVVDATNVVLGRLATKVARMLIGK
                     DKPSFTPYLDSGDHVVVINADKIRMTGNKVEQKIYYSHSGYPGGLKEVPAKRLRVAKA
                     DWMVREAVLGMLPKNKLRARRAKKLRVYRDAAGLARHAGQKPQAIAL"
     gene            1770..2189
                     /gene="rpsI"
                     /locus_tag="VGR03_03990"
     CDS             1770..2189
                     /gene="rpsI"
                     /locus_tag="VGR03_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV1993471.1"
                     /translation="MGSTGMPIAGTQTWFLGTGRRKESVARVFLRPGTGKFKINGRTV
                     EQYFPNHAWKHDAIEPLKFTNIADQVDVVVTAHGGGVGGQAGAVRMGLARAISRFNPE
                     LRPALRKNGFLTRDSRMKERKKYGQKGARKRFQFTKR"
     gene            2348..3229
                     /gene="rpsB"
                     /locus_tag="VGR03_03995"
     CDS             2348..3229
                     /gene="rpsB"
                     /locus_tag="VGR03_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV1993472.1"
                     /translation="MAVEITMKELLEAGVHFGHQTRRWNPKMKEYIFGERNGIHIIDL
                     QKTLKMFREASRFVSELAGQGRAVLFVGTKRQAQEAVAEEAKRCGAFFVNHRWLGGTL
                     TNWATLQKSIKRLKLLKAMNEDGRIKELSKKEQARLDREMKHLFQNLEGIENMIQLPD
                     AMFVIDSNAEEIAVKEARRMGVPVVSVVDTNCDPDVVDWIIPGNDDALRAIRLFTTKI
                     ADSVLEGHTAYQQSQVAEQKAAEDQALVDGVEYVDTSAYERYEKQEGDFVEGVVETPA
                     EPAEAAEATVPPDEGTH"
     gene            3342..>3540
                     /locus_tag="VGR03_04000"
     CDS             3342..>3540
                     /locus_tag="VGR03_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993473.1"
                     /translation="MSTQQPAMQIPAQLVKELRERTGAGFSDCRAALVEAGGDIEKAI
                     SILRKKGQAAAAKKAQREASEG"
BASE COUNT          844 a          903 c         1144 g          649 t
ORIGIN      
        1 cacaatacag ttcgcggcgg aatggctaga aaacaacctt ccggcgctcc ccgctgagac
       61 gccactactg aagcctctcc ttgtcgctac tccggtaccc gctcaccaag aggaagtaga
      121 gggtccgcat ggaagctatg ctcctctcgc cagctacctg gaggcgctgc cagtcgacac
      181 tgcttcagtc cctctcacgt tccagcaaat cgaagacatt ctcaagaagg agttgccacg
      241 gtcggcatct gaatatcgcg cttggtggtc taacgatcca atgaagccac agtccgccgc
      301 ctggcttgat gagggctggc ggacaacgtc cgttagcatg acagggcgtc ggttgacgtt
      361 cgttcgcacg aatgaccgag aggaggccta tatcagcttc ttcgcgaagc taaacgcacg
      421 acttgcgacg gagactgatt tccctttacg cagcgtatca ccacaagggc agaactggct
      481 tgttcttgct tctttggata gatcacggcc ggaactagcg accatagtcg cgtcattcgc
      541 gtggcgaaag cggcttcgga ttgagctcta tttagaaggt ggagacaaag atgcgaataa
      601 acaactcttc gaacggctcc tcgcacggaa aagtgagatc gagcaggttg ttggcgaacc
      661 actccagtgg gaacgattgg ataacaggcg agcgtgccga gtcgctgtct ataccagagc
      721 gcagattttg acagatgctg acagtccgac actgttggag tgggctgcca aaaaggccac
      781 ctatttctac caggcatttg aaccggaatt tcctcctggc aggtcgcccg gatagggttt
      841 acgaaaaagg gaggaaggtc gtcccgacca cagtaaagat cagacaggtc gccccactct
      901 tcatcgaagt ttgcaaggcc gatgcggtcg cgagtgggtg acggggcact cggaagcaat
      961 ccacttgact tcgcaagacg cgctgcttgt tgaaacgtaa agtccaactc tccggaagtc
     1021 cgcttgtccg agatgccagg ttcgtttcaa actgagccat tagcactgat tcaagttcgt
     1081 tgacaacatc ggtggggtac tttatgattc ttgtttgtct ctacagggaa aggtgtgact
     1141 gagcgcaatg aggacgtacg tagccaaagg tgaggaagcc gaagcgctga aagtgggcgc
     1201 gaactggttt gtagtggacg cgacgaatgt ggtgctggga cgtctggcga ccaaggtcgc
     1261 gcgcatgctg atcggcaaag acaagccgag ctttacgccg tacctggatt cgggcgacca
     1321 cgtggtggtg attaatgcgg acaagattcg catgaccgga aacaaggtcg agcagaaaat
     1381 ttattattcg cacagcgggt atccgggcgg attgaaggaa gtgccggcga agagattgcg
     1441 cgtggcgaag gcggactgga tggtgcgcga ggcggttctc ggcatgctgc cgaagaacaa
     1501 gctgcgcgcg cggcgcgcga agaagttgcg cgtgtaccgg gatgcggcgg gtttggcgcg
     1561 gcacgccggg cagaagccgc aggcaatcgc gctgtaagag agatttgttt ttttccgcac
     1621 gtagctgttc ctactaagtg gtgccttagc ggggaataag cagacgtgaa gtttgagatt
     1681 gatccgcaaa gcagcggaac ggaaatttgc tgctctgcgg agtggtatcg agatgactgc
     1741 cgcgcagtca tccatcgcga ggaggaacat tgggttctac gggaatgccc attgcgggca
     1801 cacaaacatg gtttctggga acggggcggc gcaaggagtc ggtggcgcgc gtgttcctgc
     1861 gtccggggac cggcaagttc aagattaacg gccgcacggt cgaacagtat tttccgaatc
     1921 acgcgtggaa gcatgacgcc atcgagccgc tgaagtttac gaatatcgcg gatcaggtgg
     1981 acgtggtggt gacggcgcat ggcggcggcg tgggcgggca agcgggggcg gtgcgcatgg
     2041 gattggcgcg ggcgatttcg cgattcaatc cggagttgcg gcccgcgctg cggaagaacg
     2101 gatttttgac gcgcgattcg cgaatgaagg aacgcaagaa gtacggccag aagggcgcgc
     2161 gcaagcgctt ccagttcacg aagcgttaag gattttggcc ccccgacccg gtcggggagc
     2221 cacaccgagc tccagcgggt tggcgcgagt tggcccgctg agatcacggc cggcaaggag
     2281 ccggcaatag gctgttctgt ggcgacgcga gccgccaccg caaacagcta tcctattagg
     2341 aggaaacttg gcggtagaaa ttacgatgaa agaattgctg gaggccggag ttcatttcgg
     2401 tcaccagacg cggcgctgga acccgaaaat gaaggagtac attttcggcg aacgcaacgg
     2461 catccacatt atcgatcttc agaaaaccct caaaatgttc cgcgaagcga gccgcttcgt
     2521 gagcgaactt gccggccaag gcagggcagt tttgttcgtg ggcaccaagc ggcaagccca
     2581 ggaagcggtc gccgaagagg cgaagcgctg cggtgcgttt tttgtgaacc accgctggct
     2641 gggcggcacg ctgacgaact gggcgacgct gcagaaatcc atcaaacggc tgaagctgct
     2701 gaaagccatg aacgaagacg gccgcatcaa agaactttcc aagaaggaac aggcacggct
     2761 ggatcgcgag atgaaacacc tgtttcaaaa cctcgaaggc atcgagaaca tgatccagtt
     2821 gccggacgcc atgttcgtga tcgattccaa cgcggaagaa atcgcggtga aagaagcgcg
     2881 gcgcatgggc gtgccggtag tttcggtggt ggatacgaat tgcgatccgg acgtggtgga
     2941 ctggatcata cccgggaacg atgacgcgct gcgcgccatc cgcctgttca cgacgaagat
     3001 tgcggattcc gtgctcgagg gccacaccgc ctaccagcag tcgcaggtgg ccgaacagaa
     3061 agccgccgaa gatcaggcgc tggtggacgg cgtcgagtac gtcgatacga gcgcgtacga
     3121 acggtacgag aagcaggaag gcgacttcgt ggagggtgtt gtggaaaccc cggcggagcc
     3181 tgcagaagct gcagaagcca ccgttcctcc cgacgaggga acacactaaa tagattgcgt
     3241 gggtggaaat ttcgtgaggc ccgccccggg cggcttgggg cgggccgctt cgcagatagc
     3301 gacatcaacc gagagaattc aaaaatgcaa agagaaaaag gatgagtaca caacagccag
     3361 ctatgcaaat cccagcacaa ctcgtaaaag aattgcgcga gcggacgggg gcgggattca
     3421 gtgactgccg cgcggcgctc gtggaagcgg gcggcgacat cgaaaaggcc atcagcattc
     3481 tacgcaagaa ggggcaggcg gcagcggcga agaaggcgca gcgcgaagcc agcgagggcc
//
LOCUS       DASYVO010000121         3259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_201465, whole genome shotgun sequence.
ACCESSION   DASYVO010000121 DASYVO010000000
VERSION     DASYVO010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201465"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            471..2051
                     /gene="istA"
                     /locus_tag="VGR03_04005"
     CDS             471..2051
                     /gene="istA"
                     /locus_tag="VGR03_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEV1993474.1"
                     /translation="MSNVLSQEKKQQVIALGRLGWTLRKIEQTTGVRRETASRYLKEA
                     GVAIRPPRGWGRGTPAPSSRAPDSKPANEDQVITDFGGALEALRPSGRSPTASTCETY
                     REAVELGLSRDRNAMAIYQDLVDSCGFPGSYQSVQRFVRKLRGASTPEARAVIETAPG
                     EEAQVDYGSGPMVRDPESATYRRTRLFVLTLGYSRKAVRLLVWRSSAQTWAELHEKAF
                     RRLGGTTRLVVLDNLREGVLEPDIYDPAINPLFRDVLQHYGVVALPCRVRDPDRKGKV
                     ESAVGHAKKTPLKGLRFESLADAQAYLDHWEERWADTRIHGTTKRQVSAMFAEERPAL
                     LPLPVEPFRYYKYGERTVHLDGCVEVEAAYYSAPPGWVGQRVRVQWDGTFVRLLDPRS
                     GQLLREHLRAKRGVHRIPPEDQPPRTRPGMQQLLWRAENTGPQIGVLCRQMHRRNSIT
                     AVRRIQGVLALTKKYGQAAVEQACGVALEVGFADYRLVRRYLERTPPVTLRQVDPLIR
                     QLTLYRDFIENQTKDQQL"
     gene            2048..2797
                     /gene="istB"
                     /locus_tag="VGR03_04010"
     CDS             2048..2797
                     /gene="istB"
                     /locus_tag="VGR03_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEV1993475.1"
                     /translation="MNLIELQRALRQLRLGGIAAVLETRLRQAQAEAMAPIDLISCLV
                     ADELNRRSERLLDRRRKQAQFRDPQRSLDNFDFTFNPKMNRSLVFDLAACAFINKRED
                     ALFLGPGGTGKSHLAQAIGQAAIQQGYRVIYRETHVLLENLADAIVDGTRKQFMETLA
                     TVPLLIIDDFGMRKLPPTAAEDLLEIIMRRYERASTLLTSNRPVEDWGKLLGDVAAVT
                     AMLDRLLHHGHILKCGPRSWRTKTASTAASQ"
BASE COUNT          685 a         1017 c          968 g          589 t
ORIGIN      
        1 cgaagtcccc aacgcatcaa gtccgcacct ccgagcgcaa tcagcggcgg aagttccttc
       61 ggtctttgct ccaaggccca gtccagaacg ctcaagtctg gattgaccag agcgagccgc
      121 ggcattgata cccctggaac actgagaggc tcggagcttg tggtctgaca atccgaaggc
      181 actctcaagc ggctggctac gccaccggtg ctgtgggtga cggaatcggg ccatacgctc
      241 gcgatgacga tagcctccag gggacaatcc accgggtcct gcgggatcag aaggtcagac
      301 aattcctgca attgagcagg gggcaggagc gaacggtagc cggcgtaggg tgagcgatca
      361 caccgacctc ggtaatcacc caaaaccggc caacgataat cacctgaaaa ccggccaacg
      421 ataagaccca tccaggacat tgaataacga ccgggctagt gtcggtcggg atgagcaatg
      481 tcttgagcca agagaagaaa cagcaagtca tagcactcgg gcggctggga tggaccctgc
      541 ggaagatcga gcagaccacc ggcgtccgtc gtgaaacggc atcgaggtac ttgaaggagg
      601 ccggagtagc catccgtccg ccccgaggct ggggacgagg caccccagcg ccatccagcc
      661 gcgctccgga ttcaaaaccg gccaacgaag atcaggtgat caccgacttt ggcggggctt
      721 tggaggcact ccggccgagc ggacgcagtc ccaccgccag cacctgcgaa acctaccgcg
      781 aagccgtcga gttggggctt tcgcgtgacc gcaacgccat ggccatctac caagacctgg
      841 tggacagttg cggcttcccg ggcagctatc aaagcgtgca gcgcttcgtg cgcaagttgc
      901 gcggagcgtc aaccccggaa gcacgcgcgg tcatcgaaac cgcgcccggc gaggaagcgc
      961 aagtcgatta cggctccggc cctatggtgc gcgatccgga aagcgctacc tatcgtcgta
     1021 cgcggttgtt cgttctgacg ctgggctaca gccgcaaggc ggttcgccta ttagtatggc
     1081 gatccagcgc gcagacctgg gcggaactgc atgagaaagc cttccgccga ttgggcggaa
     1141 cgacgcgcct agtggtgctc gataacctgc gcgaaggcgt gctcgagccc gatatctacg
     1201 atcccgccat caatccgttg ttccgcgacg tgcttcagca ttatggcgtg gtggcgctgc
     1261 cctgccgggt gcgcgatccg gatcgcaaag gaaaagtgga atccgccgtc ggccacgcca
     1321 agaagacacc actgaaaggt ctgcgcttcg aaagcttggc cgacgcccaa gcctatctcg
     1381 accactggga agagcgctgg gccgacacac ggattcacgg caccaccaaa cgccaagtct
     1441 cggcgatgtt cgccgaagag cggccggcct tgctaccgct gccggtcgag ccgtttcgct
     1501 actacaagta cggcgaacgt actgtgcatc tggatggttg cgtggaagtc gaagcagctt
     1561 attacagcgc gcctcccggt tgggtcggcc aacgggtgcg cgtgcagtgg gatggaacat
     1621 tcgtccgcct gctcgatccg cgtagcgggc agttgttgcg cgaacacttg cgcgccaagc
     1681 gcggagttca tcggattccg ccagaagacc agcccccgcg aacccgaccc ggaatgcagc
     1741 aactgctctg gcgcgccgag aacaccggtc ctcagatcgg cgttctctgc cggcagatgc
     1801 atcggagaaa cagcatcacc gcggtgcgcc gcattcaggg cgtgctcgcg ctcaccaaaa
     1861 agtacggtca ggcggcggtg gaacaagctt gcggcgtggc actcgaagtg ggcttcgccg
     1921 attaccgctt ggtgcgccgc tatctcgagc gcacgccgcc ggtgacctta cggcaagtcg
     1981 atcccctcat tcgtcagctc actctctatc gcgactttat tgaaaaccaa accaaggatc
     2041 agcaactatg aacctcatcg aactgcaacg cgcgttacga caactccgtc ttggcggcat
     2101 cgccgccgtg ctcgagaccc ggctccgcca ggcgcaggcc gaagccatgg cgcccatcga
     2161 tctgatctct tgtttggtcg ccgacgaact gaatcgccgc agcgaacggc tgcttgatcg
     2221 ccgccgcaag caggctcagt ttcgcgatcc ccagcgatct ctcgacaact tcgacttcac
     2281 cttcaacccc aagatgaatc gtagtctggt tttcgatctg gcagcctgcg ccttcatcaa
     2341 caaacgcgaa gacgctttgt ttcttggtcc cggcggaacg ggcaagagtc acttggccca
     2401 ggccatcggt caggccgcca ttcaacaggg ctatcgcgtg atctatcgcg aaacccatgt
     2461 gctgctggag aacttggccg atgccatcgt tgacggcacg cgtaaacagt ttatggagac
     2521 cctcgccacc gtgccgctgc tgatcatcga cgacttcggc atgcgcaagc tgccgcccac
     2581 cgcagccgag gatctgctgg agatcatcat gcggcgctac gagcgcgcca gcaccctgct
     2641 cacctcgaat cgtcccgtgg aagactgggg caaactgttg ggcgatgttg ccgcggtcac
     2701 cgcgatgctc gatcgcttgc tgcatcacgg gcatatcctg aaatgcggtc cgcgcagctg
     2761 gcgaaccaag acggcgtcca ctgcagccag ccagtgaatc gccagcagaa agccagcgga
     2821 agaggaactc tttctgttgg gggctctgcc ccaaaccccg ggatttaccg ctttgcggcc
     2881 agaatggact gcggagagcg gggacgtcgc cgtccccgtc cattccggcc cctgattcgg
     2941 cgctcaggtc gcatccctgc gttgccctat cctccgctca ggtttcgcca gtctaaacca
     3001 aggactcaat cagcgcagag gaacttatcg aaaaatcttg ccggcgcagg cctcggtaag
     3061 gtacaactga tccgttctgg aagcggtccc gttggccgct tttgaggtga ttatcgttgg
     3121 ccggttttga ggtgatcact gaggggcata gttttggtac cgtaataact aggcggacat
     3181 atgggccaaa actccaacgc gatcatcccc tatagctctt gggagcaggt caagcgaatc
     3241 atccgtgcct acgatgcag
//
LOCUS       DASYVO010000122         4334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_172223, whole genome shotgun sequence.
ACCESSION   DASYVO010000122 DASYVO010000000
VERSION     DASYVO010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172223"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VGR03_04015"
     CDS             <1..989
                     /locus_tag="VGR03_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEV1993476.1"
                     /translation="LAAGLGTRLKPLTDNRPKALVELSGRTLLEITLTRLRAFGITEV
                     IINVHHFAGQVIDYLQSHKNFNMRIEVSREDVLLDTGGGLKKAAWFFLEDSTRPSSHR
                     GDSAHPDSAQMDEPFLLHNVDVISTIDFTRMLQFHTENHALATLAVQSRESSRYLLFD
                     DSLQLRGRRTSGTPDSGTAIPGCVPQITATQNQSATASPPAQQVSSPVIPSAARNLLL
                     PREGKASEPADTPANCHSERREESAFSDSQTRHSQSLHALAFSGIHLISPRLLPLLTE
                     EGVFSIIPSYLRLAAQGEKILAFRADQYYWRDLGRPADLAQAALDLQQKALL"
     gene            1041..1298
                     /locus_tag="VGR03_04020"
     CDS             1041..1298
                     /locus_tag="VGR03_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993477.1"
                     /translation="MSPANKTVRQSVSLPANIAAQVRHLAKTRRLSSNRMLVELIENG
                     IEAEKRKQQEFFDLAERFRSTTDPEEAKRLGDELGRMVFGS"
     gene            1298..1543
                     /locus_tag="VGR03_04025"
     CDS             1298..1543
                     /locus_tag="VGR03_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993478.1"
                     /translation="MPRIARWDNLPANVRQHLIERMRDRAISIADLNQLRLWIDSQPE
                     VPAGDWFKDFGSFKFCGHGSYPKTFLLPGQAAKGEPL"
     gene            1597..2595
                     /locus_tag="VGR03_04030"
     CDS             1597..2595
                     /locus_tag="VGR03_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993479.1"
                     /translation="MRCFQITAFFFAASLFIPAARSQKTPLQIPPIDHFEIGRQFFFD
                     FGPPFHYFEIFLVRSADSGSSVKRISLTPPGDACLSPAQIESGSTTLDAQVSDLLGNS
                     NPCSIPEKELRRERKRCKGCLVFSGANVTMRVPCGTRTRLIRADVLDRDMFDAVPNTP
                     EHTSWTMQLLDRLASTLGPGVLDKPAFYSPTGDRKPAVPVEPEILGGLESGAYDNLFP
                     KSDEKPSSIYRATQIPLAQPTVKLLTSSPFAPQDPALPKYPPLPRFARVQGPVSFTLV
                     PDTAGNPGSLSFVSGNPLLQGVVREVASTWKFPKAAAGQTISATIEFALNCPTAPK"
     gene            complement(2639..3382)
                     /locus_tag="VGR03_04035"
     CDS             complement(2639..3382)
                     /locus_tag="VGR03_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1993480.1"
                     /translation="MASVSADLAHGHVIPEHSHPEDQLLFASSGVMTLRTKQGVWVVP
                     PLRAVWIPAHTPHSVAMSGPVSMRTLYFLPRLCRRLPGKCFVMNVSALLRELILHACK
                     FKRLNKRALAERRIIEIIVDQLKAVDSIPLQLPHPTDSRAMRVVKALLADPGDQRDLE
                     RLCEDCGASKRTIQRVFLEETKMNFGKWRQQLRLLHGLQLLASGEKVTAAALEAGYSS
                     PSAFISMFRKQLGTTPMRYLRDGNGRYDQ"
     gene            3533..3910
                     /locus_tag="VGR03_04040"
     CDS             3533..3910
                     /locus_tag="VGR03_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1993481.1"
                     /translation="MLASAKMVGFIPTTDYDKARAFYEGKLGFEFVSVDQFALVMNVG
                     GRKIRISKVPNFKPLQGTVLGWEVENITSVAIWLRDRGVPPEKYPFAQDQELGIWTAP
                     NGDKVAWFKDPDGNILGVNQHVA"
BASE COUNT          930 a         1477 c         1023 g          904 t
ORIGIN      
        1 tcctcgccgc cggcctcggc acccgcctca agccgctcac cgacaatcgc cccaaggccc
       61 tagtcgaact ttccggccgc actctcctag aaatcaccct aacccgcctg cgcgccttcg
      121 gcatcaccga agtcatcatc aacgtccacc acttcgccgg ccaggtcatc gactatctgc
      181 aatcccacaa aaatttcaac atgcgcatcg aagtctcgcg cgaagacgtt ttgctcgaca
      241 ccggcggcgg cctgaaaaaa gccgcctggt tcttcctcga agattccact cgccctagtt
      301 cccatcgcgg cgattccgcc caccctgatt ccgcccaaat ggacgagccc ttcctccttc
      361 acaacgtcga cgtcatcagc accatcgact tcacccgcat gctccaattc cacaccgaaa
      421 atcacgccct cgccaccctc gccgtccaat cccgcgaatc ctcccgctac ctcctcttcg
      481 acgacagcct ccaactccgc ggtcgccgca ccagcggcac ccctgacagt ggcacagcca
      541 ttcctggctg tgtaccgcaa atcacggcaa cacagaatca gtcagcaacg gcgtcaccgc
      601 ccgcccaaca agtctcctct cctgtcatcc cgagcgcagc gaggaatctg cttttgccac
      661 gcgagggtaa ggcttcagag ccagccgata cccccgcgaa ttgtcattcc gagcgaaggg
      721 aggaatctgc tttttccgat tcacagacgc gccattcaca gtcgctccac gccctcgctt
      781 tctccggcat ccacctcatc tccccccgcc tcctccccct tctcactgaa gaaggcgtct
      841 tctccatcat cccctcctac cttcgcctcg ccgcccaggg agaaaaaatc ctcgccttcc
      901 gcgccgacca atactactgg cgcgacctcg gccgtcccgc cgacctcgcc caagccgccc
      961 tcgatttgca acaaaaagct ctgttataga tttgtagctg cgatttgcac ggtcgcacca
     1021 ttctggtgca tactggtgtt atgagtccag caaacaagac cgtacgccaa agcgtcagcc
     1081 ttcccgcaaa catcgccgcc caggttcgcc acctggcaaa gactcggagg ctgagttcca
     1141 accgcatgct ggtcgaactc atcgaaaacg gcatcgaagc ggaaaagcgc aagcagcagg
     1201 aattcttcga tttggcggaa cggtttcgca gtaccaccga cccggaggaa gccaagcgcc
     1261 tcggtgatga actaggccga atggtttttg gtagctgatg cccaggatcg cgcggtggga
     1321 caatctgccg gcaaatgtcc gtcagcactt gatcgagcga atgcgcgacc gggccatcag
     1381 catcgccgac ctcaatcaac tccgcctgtg gattgactcg cagccggaag ttccggcagg
     1441 ggattggttc aaggacttcg gctcattcaa gttctgtggc cacggctcgt accccaagac
     1501 gttcttgctc cctggccagg cggctaaggg cgagccgctc tgatcggcag gaaggcttct
     1561 tttttccgca atttggcatc acaactacag cccatcatgc gatgcttcca aattaccgcg
     1621 ttcttcttcg ccgcatcgct tttcattccc gcggcccgct ctcaaaagac gcctctccaa
     1681 attccaccaa tcgaccactt tgaaattggc cgtcaattct tcttcgactt cggtcctccc
     1741 ttccactact tcgagatttt cttggtccgc tcggccgact ccggctcttc cgtcaagaga
     1801 atctcgctca cgccgccagg cgacgcttgc ttgtccccag cgcaaatcga aagcggctcc
     1861 accactttgg acgcgcaggt ttccgaccta cttgggaact caaatccctg ctcgattccg
     1921 gaaaaagaac tccgccgcga acggaagcgt tgcaaaggtt gcttggtttt tagcggggct
     1981 aacgtcacca tgcgtgtacc ttgtggcacc cgcacgcgcc ttatccgcgc agacgttcta
     2041 gatcgcgaca tgttcgatgc cgtacccaac accccggaac acacctcgtg gacaatgcag
     2101 ctgctcgatc gcctcgcctc gacccttggg cccggggtct tggacaaacc ggcattctat
     2161 tcgccgacag gagaccgcaa acccgcagtt cccgttgagc cagaaatact gggcggtctt
     2221 gaatccgggg cgtacgacaa cctattcccc aagtccgacg agaaaccatc ttctatatat
     2281 cgcgccaccc agattcccct cgcgcaaccg accgttaaac tactcaccag ctcgccgttc
     2341 gcgccgcaag atccggcgct acccaaatac ccgccactgc cccggtttgc tcgcgttcaa
     2401 ggtccggtat cctttactct cgttccagat acggcaggaa atcccggctc gctttcgttc
     2461 gtctcgggca accccttgct tcaaggagtc gttcgcgaag tcgcctccac ttggaagttc
     2521 ccgaaagccg ccgcaggcca aactatctca gcgacgattg agttcgcgct taactgcccg
     2581 accgcaccga aatagcaagc tcgttggaac gattccttct cggggactcc tctacttctt
     2641 actggtcgta ccgcccattc ccatcccgca aataccgcat cggcgtcgtc ccaagctgct
     2701 tccgaaacat cgatataaac gcactcgggc tgctataacc tgcctccaaa gccgccgccg
     2761 tcaccttttc tcccgaagcc agcagttgca gcccatgaag tagccgaagt tgctgccgcc
     2821 acttcccgaa attcatcttc gtctcctcca gaaacacgcg ctgaatcgtt ctcttgctcg
     2881 cgccgcagtc ctcgcaaagt ctttccaagt cccgttgatc tcccggatct gccagcagcg
     2941 ctttcaccac cctcatcgcc cgcgaatccg tcggatgcgg caactgcaac gggatggaat
     3001 ccaccgcctt caactgatcg acgatgatct cgataatccg ccgttccgcc aaagctctct
     3061 tgttcaacct cttaaacttg caggcatgca gaatcagttc ccgcaagagc gctgaaacat
     3121 tcatcacaaa acatttcccg ggaagccttc ggcatagcct gggcaggaaa tacagcgtcc
     3181 tcatcgaaac cggacccgac atcgccaccg agtgcggcgt atgcgcggga atccacaccg
     3241 cacgcagcgg cggcactacc caaacgcctt gcttcgtgcg cagcgtcatc actccgctcg
     3301 atgcaaataa cagttggtcc tccggatggg aatgctcagg gattacgtgg ccatgagcga
     3361 ggtcagccga tacgctggcc atctcggcaa gaagccgcct gttcggggca tggagtatgc
     3421 tttggcactt gtgcgacatt tcttgtcaca atatcgagag aatctgcgcc gggcaagtgc
     3481 tattcctgag ggccgcaaat cttctcggcg aggcattaca aggaggccta ggatgctggc
     3541 atcagcaaag atggtcggct tcatccccac aacggactac gacaaggcgc gagccttcta
     3601 tgaaggcaag ctcggatttg aattcgtaag cgtcgatcaa ttcgccctgg tcatgaacgt
     3661 cggcggccgc aagattcgca tttccaaggt cccgaatttc aagcccttgc aaggcactgt
     3721 tctcggatgg gaagtcgaaa acatcacatc cgtcgccatc tggctcaggg accgcggcgt
     3781 tcccccggaa aaatatccgt ttgcccagga ccaggaactt gggatttgga ccgcccccaa
     3841 cggcgacaaa gtagcctggt tcaaggatcc tgacggcaac atccttggcg tcaatcagca
     3901 tgtcgcctga taagattggg ccgccgcaat gtgacaaaga aagatttcca gcgagcgcta
     3961 gcctctgcca gctcatggca ggtccccaat cggcagccca ccagccccat ttcccgcttg
     4021 acgcaggggt gccgctcgcg tatagtatca agtaagagaa agtgctgtta cactcccgta
     4081 gcgccagcat cttgctggcg tttttcgttt tagcggtggg acaggcattc agcctgtcct
     4141 ccgacgacca cgatatccaa tggcgcctag gccgccaccg gatcaaggtc cagaataaag
     4201 tgatccttta agtcctttag aatccgcact tctgggcaaa caccgtgttt tgccaggttt
     4261 tagccgagat cgtccgcccg caagtccttt ggaatccgca cttgcgcgaa gccccatccg
     4321 taagctcttt agaa
//
LOCUS       DASYVO010000123         6740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_257530, whole genome shotgun sequence.
ACCESSION   DASYVO010000123 DASYVO010000000
VERSION     DASYVO010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257530"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            451..1173
                     /locus_tag="VGR03_04045"
     CDS             451..1173
                     /locus_tag="VGR03_04045"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEV1993482.1"
                     /translation="MSGYLLTLALLFLALTGSTDTKESVARLEARYRVSKTLQATFLE
                     RYIENGRVVRIEAGIAYFRRPGKMRWEYESPEKNLFVVDGKSAWFYVPADHTVTRVPA
                     KESTDWRTPLALLAGEMKVSRVCARIGLAINEKLESSDHVVLSCELRGAGAEAHKQGS
                     QEPPLQIGDAGEAVFFEIVKKTGELVRVTVKNPGGVGIEFHFTNWQADPPVDESFFRF
                     IAPPGVAIVNGELPAGNSGINP"
     gene            1267..2481
                     /locus_tag="VGR03_04050"
     CDS             1267..2481
                     /locus_tag="VGR03_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV1993483.1"
                     /translation="MGQFLCRVADAEGRVFSHVEPASSLEEARQKLADRGLYVYSVES
                     RGGRLAGLLRQKSGRQIGGSEFLILNQQFNTLIKAGLPILKSLDLLASRATSPKLRPV
                     IIQIRDRVREGKSLSEAVTEAGVFSKVYSTAILAGEKSGNLPGVLDYYIAYQRVSTGV
                     RKKILATLVYPVLLLSVAIVIVTYLVTAVIPKFALLYRDLNVELPTPTRLLIALTVDY
                     RFVVLGIVALMAVGAAVVFLWSRTEEGGTAFDRFKFRLPVIGDTLLKFQVAQFSRTLS
                     TLLTGGTPLVAGLQTASDAITSKLLRSTVGQATQMVREGESLHGALASKGVMPELALD
                     MIEVGESSGALSPMLNSVAEFYEEEVNLRLSALVSLIEPILLIFMGLLVAFILISLYL
                     PIFSFSLMGATK"
     gene            2488..4128
                     /locus_tag="VGR03_04055"
     CDS             2488..4128
                     /locus_tag="VGR03_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEV1993484.1"
                     /translation="MATTEQLPPVPGLPGDGDEQNERDVARKLAERYRFAFIDLREQR
                     IDPELFRTIPADLMFRYNFVPLELQNNVLAIAAADPGQVQMSDELPLLLGKKLLIRVA
                     TARQIGDILKRTEQSQRVLEQATEAFTLQTSKDEDEGDETISGDRLTRDSTASPVVRL
                     VETIIFTALERRASDIHIEARDSEVAVKYRIDGVLQHAMQPIAKEWHSTVLSRIKVLS
                     ELDIAERRVPQDGRFRVKYKGRYIDLRVSIMPASHGEDAVLRVLDKETLSEKFHSLTL
                     DVVGFSAEETRRFRRYIREPYGMVLVTGPTGSGKTTTLYAAINEIKTDEDKIVTIEDP
                     VEYQLRGITQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDQETAQIAIQSALTGH
                     LVFTTVHANNVTDVIGRFINMGVEPYNFVSALNCIMAQRLVRVICTNCKKPRKYSPEE
                     LKEAGLDPVVWSNVTFAEGAGCLECSGTGYHGRTAICELLDLTDRIREMIVDRRPTSE
                     IKRVACEEGMITLRESGLAKIRAGITTVKEINKVTFVE"
     gene            4121..5098
                     /locus_tag="VGR03_04060"
     CDS             4121..5098
                     /locus_tag="VGR03_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993485.1"
                     /translation="MSSTTAFRQGIYQFEWVRRVARWLDAMPHASLSIEVAPERIAAA
                     RWSRTGSLDGYAVESLPVGALIPSAVEANIVNGAAVKAAVANVCDRLRSRDEDVAMIL
                     PDTVIRVFVQHFEDFPRSAAEAVPMLRWKLKKSVPFESDETLISYMRQAPREAGVDVV
                     TALARLRIIREYESLVEGIGLHPGVVLSSSLAAISLLEDRKPTLLARVSGSTLTTAIV
                     RRGVLCGYRCTELPAQGANLTPQMLLEEVFPVAAYYQDTWQEAIQSVRVAGFGTRLPE
                     FVRTLEDEFRCEVRSLLHAAVSDGRIKEDARPLADRELEGLIGWMLQRG"
     gene            5132..5704
                     /locus_tag="VGR03_04065"
     CDS             5132..5704
                     /locus_tag="VGR03_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993486.1"
                     /translation="MRVRLNLATKALETHRRFLVGAGLAAAFAGLAFFGLGWHVYSAR
                     KVDAELRARTEKTRQEMAKLETQRKDLERYFSQKDIANLHDRAAFINTILDARSFNWT
                     LMFMDLERILPAGVRVISIEPKQVAGHVEVKLSVGATSDEAELKFEHALEESKEFTEV
                     QIQHVSSPSSIGNQSGDQKVVQLTAVYSRT"
     gene            5704..6264
                     /gene="pilO"
                     /locus_tag="VGR03_04070"
     CDS             5704..6264
                     /gene="pilO"
                     /locus_tag="VGR03_04070"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEV1993487.1"
                     /translation="MRRDFTVRKRTILAGVILLVLADVALAAYSWQLSSAPQAPQQQL
                     AVETKQHDLLKADIKRAQDIRDNIPAIQKDCDHFEQSLFPASSGYSAVRSELGVTAKK
                     SGIQLEDLSLKETKISNRGMTEVTIDATVNGDYKSVIGFLNGLQRSPNLFAVDALTLA
                     SESANQASTNVIKVALHLKTYFRTAA"
     gene            6261..>6740
                     /locus_tag="VGR03_04075"
     CDS             6261..>6740
                     /locus_tag="VGR03_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993488.1"
                     /translation="MKQKKQLAVLVVLLLIAGVIWFFYFDHDKPIVAADTGSAAQNPQ
                     LLSVENPSLHKAAVDKARKTEYKSGGRNIFSRDLPIPPRSKETIQKLKEQRTQIVQQV
                     PVEIKVPPLPVKFFGFGTVPNGTARLAFFSDGEDIYVVKEGELLMNRFRILKIGNANL
                     "
BASE COUNT         1421 a         1981 c         1882 g         1456 t
ORIGIN      
        1 tgccgttagt agcgtacgat ccatctcgac atcggaaacc gcttatgcaa cctcctatcc
       61 gctcatcgga tattcagtcg ctctcacaga tctcggggat ggcggctcaa gtaattgcgt
      121 ggccataaat acgcgggctt gcctcattga tagcattcta gcgggcggca caaagagcgg
      181 gttcttattc acctacgcca acgatggaag tacacccgca gtcggataca ccgtcaatgc
      241 cgatccgagt agccgtggag taacgggccg gaaaagcttt ttcagcaatc agccgggtga
      301 gatccgctac aacaccgcgg ccgcggcaac gttggtggac cccccaatct gattctttgg
      361 aaccttgaat ttcagcggct ttcaagcgaa ttacctctgc tctgacaact cccagtgatg
      421 tgcaagttca tgtgctgcta gactgcttac atgtctggat accttcttac gctggctctt
      481 ctttttctcg cgctcaccgg cagcaccgat acgaaagaga gcgttgctcg tctggaggcc
      541 aggtatcgtg tgtcaaagac gctgcaagca acatttctcg agcgatacat cgagaacgga
      601 cgtgtcgtcc gcatagaagc aggcattgcc tatttccgcc gcccaggcaa gatgcgctgg
      661 gaatacgaat ctcctgaaaa aaatcttttc gttgtggacg gcaagtcggc ctggttctac
      721 gttccggcag accacaccgt cacgcgcgtt cccgcgaagg aaagcacgga ttggagaact
      781 cctctggcgc tgcttgcagg agagatgaag gtgtcgcggg tgtgcgcccg gatagggctc
      841 gccatcaacg agaagctgga atcttcggac cacgtcgtac tctcctgcga gcttcgcggc
      901 gcgggcgccg aggcgcacaa gcaaggttct caagagcccc ctctgcagat aggagatgca
      961 ggcgaggcag tcttcttcga gatcgtcaag aagacgggag aactggtgcg cgtaaccgtc
     1021 aagaaccccg gaggcgtggg catcgaattt catttcacca actggcaggc cgatccacct
     1081 gtcgacgagt cgttcttccg cttcattgct ccgcccggtg tggccatcgt gaatggagaa
     1141 ttacccgccg gtaatagcgg gataaatcct taggcgtgca ggcattccgg ccccctttcc
     1201 tgcgaggaac agccaacctc ccgggccttc cccgcatatc agctagatga ggtaaactga
     1261 gaagctatgg gtcaattcct ctgccgcgtg gcggatgccg aaggacgtgt cttttcgcac
     1321 gttgaacccg caagttccct ggaagaagcg cgccagaagc tcgcggacag gggattgtac
     1381 gtctattcgg tggaatctcg tggcggccgt ctggccggtc tgcttcgcca gaaaagcgga
     1441 cgccagattg gcggttcgga attccttatt ctcaaccagc aattcaacac gcttatcaaa
     1501 gccggcttgc cgatcttgaa atccctggat cttctcgcca gccgagccac gtcgccgaaa
     1561 ctccgtccgg tcatcatcca gattcgagac cgcgtacgcg aaggaaagtc gctttccgag
     1621 gcggtcacgg aagccggtgt gttttccaaa gtctactcga cggccatcct tgcgggagaa
     1681 aaaagcggga atttgccggg tgtcctggac tattacatcg cctatcagcg cgtcagcaca
     1741 ggtgtccgca aaaagattct cgctacgttg gtttaccccg ttttattgct cagcgtggcg
     1801 atcgttatcg tgacttacct ggtcaccgcg gtgattccaa agtttgctct gctctaccgc
     1861 gacttaaacg tggagctccc cacgcccacg cgattgttga ttgccttgac ggtcgactat
     1921 cgctttgttg ttctgggaat cgtggcgctg atggccgtgg gtgcagcggt cgtttttctg
     1981 tggtcccgca cggaagaagg cggaacggct ttcgatcgtt tcaagttcag gttgccggtg
     2041 attggcgaca cgctccttaa atttcaagtc gcacaatttt cccgcacgct ttcgacgcta
     2101 ttgacgggcg gcacgccgtt ggtggccgga ctccaaacgg caagcgatgc catcaccagc
     2161 aagttgctgc gttccacggt cggccaagca acgcagatgg tacgcgaagg agaatctctg
     2221 cacggcgccc tggcgtccaa gggcgtcatg ccggagttag cactggatat gattgaggtc
     2281 ggcgaatcga gcggagcgct ttcccccatg ctgaacagcg tggccgagtt ttacgaagaa
     2341 gaggtcaact tgcgtttgtc ggcgctcgtt tccctcatcg agccgattct attaatcttt
     2401 atgggattgt tagtcgcgtt cattctgatt tcgttgtatc tacccatctt ttctttctcc
     2461 ctgatggggg cgacaaagta aaggatcatg gccaccacgg aacaacttcc tccagtgccc
     2521 gggctgcccg gcgacggtga cgaacagaac gaacgcgatg tcgcgcgtaa actcgcggaa
     2581 agataccgct tcgctttcat cgatctccgg gagcagcgca tcgatccgga attgtttcgt
     2641 accatccccg cggacctgat gttccgctac aacttcgtgc ctcttgaact gcagaacaat
     2701 gttttggcca tcgcggcggc agatcccggc caggtgcaga tgagcgatga gttgcctctc
     2761 ctgctgggca agaaactttt gattcgcgtc gctaccgccc gccagatcgg cgacattctg
     2821 aaacgcaccg agcaatcgca acgcgtgctt gaacaagcca ccgaagcttt cactcttcag
     2881 acttccaagg acgaagacga aggcgatgag acgatttccg gcgaccgttt gactcgtgac
     2941 agcaccgcca gcccagtggt gcgcctcgtg gagactatta tcttcaccgc actcgagcgc
     3001 cgcgccagtg acatccacat cgaagcgcgc gactccgaag tcgccgtcaa ataccgtatc
     3061 gacggcgtgt tgcagcacgc catgcagccc atcgccaagg aatggcattc cacggtgctc
     3121 tcgcgcatca aggtgttgag cgagctggat attgccgagc gccgcgttcc ccaggacggc
     3181 cgcttccgcg tgaaatacaa ggggcgctac atcgatctgc gcgtctccat catgcctgcc
     3241 agtcacgggg aagacgcggt tctccgcgta ctcgacaaag aaacgctctc ggaaaagttc
     3301 cacagcctga cactggacgt tgtcggtttc tccgcggaag aaacccgccg tttccggcgc
     3361 tacatccgcg aaccgtatgg catggtcctg gtcacgggtc ccacgggttc cggcaagacc
     3421 accacgctct acgcggccat caacgagatc aagaccgacg aggacaaaat cgtcaccatc
     3481 gaagatccgg tggagtatca gctccgcggg atcacgcaga ttccagtgaa cgagaagaag
     3541 ggtctcacct tcgcgcgcgg cttgcgctcc atcctgcgcc atgaccccga caaaatcatg
     3601 gtcggtgaaa tccgcgacca ggaaaccgcc caaatcgcca tccagtcggc cctcaccggc
     3661 cacctcgttt tcaccaccgt gcacgccaat aacgtgaccg acgtcatcgg ccgcttcatc
     3721 aacatgggcg ttgagccata caactttgtt tccgctctaa actgcatcat ggcgcagcgg
     3781 ctcgttcgcg tcatctgtac gaactgcaag aagcccagaa aatattctcc cgaggaattg
     3841 aaggaagcgg gcctcgatcc ggttgtgtgg agcaacgtga cgtttgccga gggcgccggg
     3901 tgcctggaat gctccggtac cggttatcat ggccggacgg cgatttgcga actgctcgac
     3961 ttgaccgacc gcatccggga aatgatcgtc gatcgccggc cgacttcgga gatcaagcgc
     4021 gtcgcttgcg aagagggcat gatcactctg cgcgaaagcg ggctggccaa gattcgtgcg
     4081 ggcattacga ccgtgaaaga aatcaacaag gtgactttcg ttgagtagca ccactgcatt
     4141 ccggcaggga atttaccagt tcgagtgggt gcgccgcgtt gcgcgctggc tcgatgccat
     4201 gccgcacgcc tccttgtcca tcgaagttgc tccggagcgc atagcagcgg cccgctggag
     4261 ccgcacgggt tcgctggatg gctatgccgt cgaatctttg cccgttggag cgcttattcc
     4321 ctcggccgtc gaagccaaca tcgtgaacgg cgccgccgtc aaggccgcag tcgccaatgt
     4381 ctgcgaccgt ttgcgttccc gggatgaaga tgttgccatg atccttcccg ataccgtgat
     4441 tcgcgtcttc gtgcagcact ttgaggattt cccgcgctcg gcagccgaag cagttccgat
     4501 gttgcggtgg aagctgaaga agagcgtgcc cttcgaatcc gacgagacgc tgatctccta
     4561 catgcgacag gcgccccgcg aagccggcgt ggacgttgtc accgcgctcg cccggctccg
     4621 catcatccgg gagtacgaat ccctagtcga gggcatcggg ttgcatcctg gcgtagtgct
     4681 gagttcgtct cttgccgcca tctcgctgct cgaagatcgg aagccgacgc tccttgcgcg
     4741 cgtctcgggt tccaccctga ccaccgcgat cgtgcgccgc ggcgtcttgt gcggctatcg
     4801 ttgcaccgaa ctcccggcac agggcgcgaa cctcacgccc cagatgcttc tcgaagaggt
     4861 ttttccggtg gcggcttatt atcaggatac ctggcaggag gccattcagt ccgtccgcgt
     4921 cgccgggttt ggcacgcgcc tccccgaatt tgtacggact cttgaggacg aattccgctg
     4981 cgaggttcgt tctttgctgc acgccgcggt ttctgatgga cgcatcaaag aagatgctcg
     5041 tccgctggcg gatcgcgaat tggaaggctt gatcggctgg atgctgcagc gtggctagta
     5101 tcggctttta cggatcggga caagagagcg tatgagggtt cggcttaact tggcgactaa
     5161 ggcgctggaa acccacaggc gtttcctggt gggtgctggt ctcgccgctg ctttcgccgg
     5221 tctcgctttt ttcgggctgg gatggcacgt gtattctgcg cgcaaagtcg atgctgagct
     5281 gcgcgctcgt acggaaaaaa cgcgccagga gatggccaaa ctcgagacgc agcgcaagga
     5341 cctggagcgt tattttagtc agaaggacat cgccaatctg cacgatcgcg ccgccttcat
     5401 caataccatc ctcgacgcgc gcagcttcaa ttggacgctg atgttcatgg atctggagcg
     5461 catcctgccg gccggcgtac gcgtcatcag catcgagccc aagcaggttg ccgggcacgt
     5521 cgaagtaaaa ttgagcgtgg gcgccaccag cgacgaagcg gagttgaaat tcgagcatgc
     5581 cctcgaggaa tctaaggaat ttacggaagt tcaaatccag catgtttctt cgcccagttc
     5641 aatcggcaac cagagcggcg atcaaaaggt cgtccagttg accgccgtct attcgaggac
     5701 ttgatgcgtc gcgatttcac agtgcggaaa cgaacgattc tcgccggcgt gattcttctc
     5761 gtgctcgcgg atgttgctct ggcggcttac agttggcagc tttcctctgc cccgcaagcg
     5821 ccccagcagc agcttgccgt ggaaacaaag caacatgacc tcttgaaagc cgatatcaag
     5881 cgcgcccagg atatccgcga taacattccc gctatccaga aagattgcga tcacttcgaa
     5941 caatcgctct ttccggcaag ttcaggctat tccgccgtca ggtctgaact cggcgtcact
     6001 gccaagaaga gtgggatcca gctcgaggat ctctcgctca aggaaacgaa aatttccaat
     6061 cgcgggatga cggaagtcac catcgatgcc accgtcaatg gcgactataa gagcgtgatc
     6121 gggtttctga atggcttgca gcgctcgccc aatctgtttg ccgtggacgc tttgacactg
     6181 gcttccgaga gcgccaatca agcatccaca aatgtcatca aggtcgcgtt gcacctcaag
     6241 acttatttca ggaccgcagc atgaagcaga agaaacaact cgccgtgctt gtcgttctcc
     6301 tgctgatcgc cggcgtcatc tggtttttct atttcgacca tgacaaaccc attgtggccg
     6361 ccgataccgg ctccgccgcc caaaatcctc agctactttc cgtggaaaac cccagtctcc
     6421 ataaggctgc ggtcgacaag gcccgcaaga ctgaatacaa gagtggcggg cgcaacatct
     6481 tcagtcgaga tcttccgatt ccgccgcgct ctaaagaaac gattcagaaa ttgaaggaac
     6541 agcgcactca gatagttcag caggtgccag tcgaaataaa ggtgccgccg ctacccgtga
     6601 agttcttcgg ctttggcacc gttcctaacg ggaccgctcg cctcgccttc ttcagcgacg
     6661 gggaagacat ttacgtcgtc aaagaaggcg aactcctgat gaatcgcttt cgcattctca
     6721 aaattggcaa cgccaatctc
//
LOCUS       DASYVO010000124         6149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_172259, whole genome shotgun sequence.
ACCESSION   DASYVO010000124 DASYVO010000000
VERSION     DASYVO010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172259"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VGR03_04080"
     CDS             <1..928
                     /locus_tag="VGR03_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV1993489.1"
                     /translation="QTPLTMMEVANWFEELGFPPGVINIVNGFGETAGAALVAHPGVD
                     KIAFTGSAAVGKIIVKSAADTLKRVTLELGGKSPNIFFDGADWEAAVDGALFGVFINQ
                     GEVCSAGSRILVEKKIYSKFVDAMTEKAKRIKLGPPLERETKMGPLVSKEQYDRVNSY
                     LELGKKEAKVAIGGGRPKQFAKGFYVEPTIFYDVDNNARIAREEIFGPVASVIPFEGE
                     PEAIRIANDTPYGLAAAVWTRDIYKAFRVVKSLRAGIVWVNHMQPTYVEAPWGGYKQS
                     GFGRELGPWGMEEYLETKQVFVNLDETPIGWY"
     gene            975..2324
                     /gene="gabT"
                     /locus_tag="VGR03_04085"
     CDS             975..2324
                     /gene="gabT"
                     /locus_tag="VGR03_04085"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEV1993490.1"
                     /translation="MKTIQIRTEIPGPQSRALMARREAAIPRGPSNATPVFAARADGA
                     TIEDVDGNRYLDFAGGIGCLNVGHRSPRVLSAIREQLEKHLHLCFAVTPYESYVAVAE
                     KLNALAPGNFPKKTILLNSGAEAIENSIKIARAYTKRPAVICFEDAFHGRTLLAMSLT
                     SKTHPYKAGFEPFASDIYRIPFAYPYRGEKGATAETFAHYLEDAFKRSVAPESVAAVI
                     AEPVLGEGGFIIPPAGYFKLLQNICRQHGILFIADEVQSGFARTGKWFASEHFGIEPD
                     LITMAKSLGGGMPIAAVTGRAEIMDAPGVGALGGTYCGHPLSCAAALAAIETIEKDGL
                     LARSTAIGKHFERRAQAWQKKWPLVGDVRGLGGMCALELVRDAQTREPADSETKAIAK
                     FCYQHGLITITAGTFNNVIRILVPLVVTDDQLDEGLGVLEAALASVAEHKQLALSHA"
     gene            complement(3182..4267)
                     /locus_tag="VGR03_04090"
     CDS             complement(3182..4267)
                     /locus_tag="VGR03_04090"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEV1993491.1"
                     /translation="MRNVWKCAVGLLVGLGVLAGNAGAQFKNGSQATELNIPRVSQRG
                     TVSQRIGLTDITIAYHRPAVGGREIWGKTVPFGKVWRAGANENTTITFSDDVSIEGKP
                     LAAGTYGLHTIPDKDQWTIIFSKNSTSWGSFSYDEKEDALRVNTKPQAADFREALTYT
                     FEEVKPDSAAATLRWEKLAVPFRISVDVKAVVLRSIKNELRSVGGFTWGGYDEAAQWC
                     LDNNYNLEEALKWEDTSIQNEERFENLETKSRILDALGRKEESAKAFSKAFDKASAIQ
                     LYSYARGLQRSGNAKRAFELYPQVPKKDANHWISHLALARISSNTGDFPTAAKEMTMA
                     ISGAPEATKPFLQPLLKRLEAKDDINK"
     gene            complement(4550..5416)
                     /locus_tag="VGR03_04095"
     CDS             complement(4550..5416)
                     /locus_tag="VGR03_04095"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1993492.1"
                     /translation="MRDSSGQPISAAASVKLYKEGIPADQGMTSHGRAFFILPSLGDY
                     TLVVDAVGYRAAQKEVSLRVAVKAEVDIYLQRVPAANETAGVPGRPLLAPKAKEALDK
                     GLQALSANKLTDAEKYVGEAVKLAPGHPDVLFVQGVLCLSQHNWTQAQSVLEKAAQMD
                     PSNARVFSALGMALTDQGKYEEAITPLEKSLQLEAGGWETHWALGKAYYYRQQYNQAL
                     KTSQLALTESNGKAPQIELLVAQSLTAVGRYDDAAQTLRDFLKSHGDRPEAATARRWL
                     AGLAKNGKIRQD"
     gene            complement(5640..>6149)
                     /locus_tag="VGR03_04100"
     CDS             complement(5640..>6149)
                     /locus_tag="VGR03_04100"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1993493.1"
                     /translation="EAIAEFQAAAKAAPREPNVNFGLGYLCWKLRQYDDARPAFEREL
                     SVDPDHAQALAYLGDIEMKSGNLEKAVLSLKKAVQLKADIHIAYADLGAIFMEQKHYP
                     DALAALQRAVKLDPAQPDTHFRLGRLYQAMGNTPAANQEFAKVRELHQKTSEDILEKM
                     SGSPPPLQP"
BASE COUNT         1247 a         1960 c         1613 g         1329 t
ORIGIN      
        1 gcagaccccg ctcaccatga tggaagtcgc caactggttt gaagaattgg gttttccgcc
       61 cggcgtcatc aacattgtca acggatttgg cgagaccgcg ggagccgcgc tcgtcgctca
      121 tcccggcgtg gacaaaatcg cattcacagg cagcgccgct gtcggaaaaa tcatcgtcaa
      181 gagcgccgcg gacaccctca agcgcgtcac gctcgagctt ggaggtaaat ctcccaacat
      241 cttcttcgat ggcgccgatt gggaagccgc cgtcgacggc gcgctctttg gcgttttcat
      301 caatcagggc gaagtttgtt ccgcgggcag ccgcattctc gtcgagaaaa agatctattc
      361 aaaatttgtg gatgccatga cggagaaggc caagcgcatc aagctgggcc ctccgctcga
      421 gcgtgaaacg aagatggggc cgctggtcag caaagagcaa tacgaccgcg tcaactccta
      481 cctcgaactc ggcaagaagg aagcgaaggt agccatcgga ggcggccggc cgaaacagtt
      541 tgccaagggt ttttacgtcg agccgaccat tttctacgat gtggataaca acgcacgcat
      601 cgctcgcgaa gagatcttcg gccccgtcgc ttccgtgatt ccctttgaag gcgaaccgga
      661 ggccattcgc atcgccaacg ataccccgta cggactcgcg gcagccgtct ggacgcgcga
      721 catttacaaa gcgttccgcg tcgtcaaatc gctccgcgcc ggcatcgtct gggttaatca
      781 catgcaaccg acgtatgtcg aagcgccatg gggcggttac aaacagtccg gcttcggccg
      841 cgaactcggt ccctggggca tggaagaata tctggaaacc aagcaggtgt tcgtcaatct
      901 cgacgagacg ccgatcggct ggtactagaa atcattacgg cggcttcgtg gcctccggca
      961 agaaggaacg cgcaatgaaa acgattcaga ttcgcacgga aatacccgga ccccagtcgc
     1021 gcgcgttgat ggcccgccgc gaagccgcca tcccgcgcgg cccttcgaat gccacgccgg
     1081 ttttcgccgc gcgcgccgac ggcgccacca tcgaggatgt cgacggcaat cgctatctcg
     1141 atttcgccgg cggcatcggc tgcctcaacg tcggccaccg ctctcctcgc gtgctctccg
     1201 cgatccgcga gcagctcgag aagcatttgc atctctgctt tgccgtcact ccctacgaaa
     1261 gctacgttgc cgtcgcggaa aaactcaacg ccctagcgcc cggcaatttt ccgaagaaaa
     1321 ccatccttct taactccggc gccgaagcca tcgaaaactc catcaagatc gcgcgtgcct
     1381 acaccaagcg ccccgccgtt atttgtttcg aggacgcctt ccacggccgc acgcttctcg
     1441 ccatgtcgct gaccagcaag acgcatccat acaaagcggg cttcgagcct ttcgccagcg
     1501 acatctaccg cattcctttc gcctatccgt accgcggcga aaaaggcgcc actgcggaaa
     1561 cttttgcgca ttatctcgaa gacgctttca aacgatccgt ggctccggaa tccgtcgccg
     1621 ccgtgatcgc cgagcccgtt cttggcgaag gcggcttcat cattcccccg gccggctact
     1681 tcaagctcct gcaaaatatc tgccgccagc acggcattct cttcatcgcc gacgaagtgc
     1741 aatccggctt cgctcgcacc ggcaaatggt tcgcctccga acatttcggc atcgagcctg
     1801 atttgatcac catggccaaa tccctcggcg gaggcatgcc catcgccgca gtcaccggtc
     1861 gcgccgaaat catggacgcc cccggtgtag gcgctctcgg cggcacctac tgcgggcacc
     1921 ctctttcgtg cgccgccgcg ctggccgcca tcgaaacgat tgagaaagac ggcctgctcg
     1981 cgcgttccac cgccattggc aagcattttg aacggcgcgc ccaagcctgg cagaaaaaat
     2041 ggcctctggt cggcgacgtc cgaggcctcg gcggcatgtg cgccctcgaa ttggtgcgtg
     2101 acgcgcagac acgcgagccc gccgactccg aaaccaaggc catcgccaaa ttttgctacc
     2161 agcacggcct catcaccatc accgccggca ccttcaacaa cgtcattcgc atcctcgttc
     2221 cgctcgtcgt cactgacgac caactcgacg aaggcctcgg cgtcctggaa gccgctcttg
     2281 cttccgttgc cgagcacaag cagctggcgc tttctcacgc ctaatcattc aaccgagctc
     2341 gacggcgcgc acccagccgc actggagcaa ccatgccaga aagaagtacg gaaagtagaa
     2401 aatctgcgat gagcgatttt attcctactg ggctcccggc ctgtcgcgct gattttcgcg
     2461 gattggtttt gatcgagagc gcgcagccgt cccataaatg aaagtcaaaa gcctcacacg
     2521 caaatacggc gtgatggggc acccaaattc gtatcagaat ttaggcgggt ggcgaagaga
     2581 caagctggga ggacggaccg ggggtgccgc acccttcggt tttaagggtg cgggttttga
     2641 ctttgctttt ttctcgctcg tctcgtgccc gcggacgcca ttcaacccgc caagacaacc
     2701 gttcgacccc caatttcgac cattcactgc aaaacactta acaacttgat tatggcgtgc
     2761 tattctctcc cgtgtttgcc ggcatacagt ctcaccgacc tctcgattct gtttccagcc
     2821 taacccctac ttcctacccc ctattcccca ttcttcccca cgctgaccac tgtccgccga
     2881 tcaccgccac tcttcctcta agtccctacc cttcaatctt tttgccgacc cccaccctct
     2941 aaccccaatc gcatcaatcc tttacaaaaa catggaaggg gagggcgtac cttcggcgtt
     3001 ccgatccctc tccccctgtc ccctgtccca ctatccctct aaattcaata gaatcatatc
     3061 gtttgccgac ccccacccac ttaccacaat caaatcatat cgttacaaaa acatgggagg
     3121 gaggggggta cctccatctt tccggcgcct accccctatt tcctatcccc tctcttctca
     3181 actacttatt gatgtcgtcc tttgcttcca agcgcttcag caatggctgg agaaacggct
     3241 ttgtcgcttc cggcgccccg ctgatggcca tcgtcatttc cttggccgcc gtcgggaaat
     3301 ctcccgtgtt cgaggagatt cgcgcgagcg ccaggtggct gatccaatga ttcgcatcct
     3361 tcttgggaac ctggggatac agctcaaacg cgcgcttggc gttccccgat cgttgcaggc
     3421 cgcgagcgta ggagtacagt tgaatcgcac tggctttatc gaacgccttg gagaacgctt
     3481 tggcggattc ttcttttcgt cccagcgcgt cgaggatgcg agatttggtc tccaaatttt
     3541 caaaccgctc ttcattctgg atcgaagtgt cctcccattt gagcgcttcc tccaggttgt
     3601 aattgttgtc cagacaccat tgcgccgcct cgtcgtatcc gccccatgtg aatccgccga
     3661 cactccgcag ctcgttcttg atgcttcgca gaacaacggc cttcacgtcc acggaaatgc
     3721 ggaagggaac ggccagcttc tcccaccgaa gagttgccgc cgccgagtcc ggcttgacct
     3781 cctcgaaggt gtaagtaagc gcttcacgga aatccgcagc ctgcggcttg gtattcacgc
     3841 gcagcgcgtc ctctttctcg tcgtagctga agctgcccca ggaagtcgag ttcttcgaaa
     3901 aaataatcgt ccactgatct ttgtccggaa tggtgtgcag cccataagtg cccgccgcaa
     3961 gcggtttccc ttcgatggag acatcgtcgg aaaaagtgat ggtcgtgttc tcattcgcgc
     4021 ccgcgcgcca caccttcccg aaaggcaccg tcttgcccca aatttctcgc ccgcctaccg
     4081 ctggccggtg atacgcaatg gtgatgtccg tcagcccaat ccgctgcgac accgtcccgc
     4141 gctggctcac gcgcggaatg ttcagctccg tcgcctggct tccattcttg aattgcgcac
     4201 ccgcattccc cgccaaaaca ccgagtccca ccaacaggcc caccgcacac ttccacacgt
     4261 tgcgcataat cacaccatct cctctcgatc tgaaactcac agatattttt gcggagtcgc
     4321 gtccgcttta caccccagta gcgatagacg cgccttccaa agaggagtga gcaaggaagc
     4381 acgcggcgcg cagcgcgaat cgtgtcaatt cagaataaat tttggaggat ttacggccag
     4441 tgacagggaa tcgcagtctc catactctga cagagggcgg ttgctctcca tgggcttcgg
     4501 gtttctaagc ccacggtgga agtgggacag agcgttcgag aagcaggttc tagtcttgcc
     4561 gaattttccc gtttttcgcc aagcccgcca gccagcgtcg tgcggtggcg gcttcagggc
     4621 gatcgccgtg gctcttcagg aaatcacgca gagtctgcgc tgcgtcgtcg taccgcccca
     4681 cggcggtgag cgactgggca acaagaagct cgatctgggg cgccttcccg ttcgactccg
     4741 tcaacgccag ctgcgaagtt ttcagcgctt ggttatattg ttggcgatag taatacgctt
     4801 tgcccagcgc ccaatgtgtc tcccagccgc cagcttccaa ctgcaacgat ttctcgagag
     4861 gggtgatggc ctcttcgtat ttcccctggt cggtaagggc cattcccagc gccgaaaaaa
     4921 cgcgtgcgtt actcggatcc atctgcgcgg ccttctccaa aacgctttgc gcctgcgtcc
     4981 aattgtgctg gctgaggcac agcacccctt gcacaaaaag cacgtcaggg tgcccgggcg
     5041 cgagcttcac cgcttcgccc acatacttct ctgcgtctgt cagcttgttg gcgcttaacg
     5101 cttgcaatcc tttgtcgaga gcctctttcg ccttcggcgc gagcagcggc ctacccggca
     5161 cgcctgcggt ttcgtttgca gctgggacgc gttgcaggta aatgtcaact tcggctttca
     5221 cggcgacgcg caaagacact tccttctgtg cggccctgta tccgaccgcg tccacaacaa
     5281 gcgtgtaatc gcctaaggaa ggcagaatga agaatgcacg tccgtgcgaa gtcattccct
     5341 ggtcggctgg gattccttct ttgtagagtt tcacactcgc ggccgcggag atgggctgcc
     5401 cagaactgtc gcgcacagtc agcgagattt ccgccctgtt tccgcggaga acagttcctt
     5461 cacttccgct atcctggcag aaagccgaag cagtcgacaa gaagaggcaa ggaactacgg
     5521 ggagcaggaa tcgcacgatc cacaggggtg cgtgttgctt tggatgacac ataaattctg
     5581 gtgaaaaact ggtcaaagag tagtcccgtg cgccgggcac gtcaaggaat aataccccct
     5641 tacggctgca acggaggcgg cgacccggac atcttttcaa ggatgtcttc gctcgttttc
     5701 tgatggagtt cgcggacttt ggcgaattcc tgattagccg ccggcgtgtt gcccatggcc
     5761 tgatacaagc gccccaggcg gaaatgcgtg tcgggctgag cggggtccaa cttcacggcc
     5821 cgctggagtg cggcaagcgc gtccggataa tgtttctgct ccatgaagat ggcgcctaga
     5881 tcggcatagg cgatgtgaat gtcggctttc agttgcacgg ctttcttgag cgagaggacg
     5941 gccttttcca gattgccgct cttcatttcg atatcgccga gataggccag tgcttgagcg
     6001 tgatcaggat cgaccgatag ctcgcgttcg aacgcgggtc ttgcgtcatc atattggcgc
     6061 aacttccagc acagatagcc gagtccaaaa tttacgtttg gctcccgtgg ggcagccttc
     6121 gcggccgcct ggaattccgc gatcgcttc
//
LOCUS       DASYVO010000125         3495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_142853, whole genome shotgun sequence.
ACCESSION   DASYVO010000125 DASYVO010000000
VERSION     DASYVO010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142853"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            436..1719
                     /locus_tag="VGR03_04105"
     CDS             436..1719
                     /locus_tag="VGR03_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEV1993494.1"
                     /translation="MSLPPIPGLIPVYEDYARCIHCGLCLNACPTYRLWNLEADSPRG
                     RIHQMIHVELDKQPITDSFVDHIDKCLDCRACETTCPSGVEYGKLVEHARARIERDYP
                     RSWLARVTRDFVFRHLLPDPHRIIDFARVIRFYQRSGMQTIARGIGILKLLGLAERER
                     LLPRIDDDFFYRRLGWTFPAAGPRRARVAFFAGCVANVTFSQLNEATIRVLTANGCEV
                     VVPSGQLCCGALAAHAGVRDAARDLARKNLSVFLREDFDAIVTNAAGCGSTLKEYDHL
                     FSQDEEEHNQARVFAAKTRDVTEFLAALGLTAKLKPLPLRVTYQDSCHLHHGQKIREA
                     PRMLLRAIPNLDFVELPYSEICCGSAGVYNVTQTETSLELLAEKMRHAQSTRAQTIVT
                     ANPGCLLQLRAGAAIHRTNQEVLHVVELLDRAMQP"
     gene            complement(1911..2792)
                     /locus_tag="VGR03_04110"
     CDS             complement(1911..2792)
                     /locus_tag="VGR03_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993495.1"
                     /translation="MNRKMYLYSVLSGALLLNAGVVRAQEPEAPLPPGPVVMAQGPGP
                     EAGMIGERIELLGFEGMHGGKVVTGVPFSAVAVSESTQTLADGNHIARKTETNLYRDS
                     QGRFRKEVTLPAIGPLATSGQPKSFVFINDPVAGTNFILHPDEKTAEKMEMPFGKMKD
                     VIKGKMEYHVQQAIGDGSLKKEDLGAQTIAGVSAQGTRITHTIPAGQMGNEKPITIVS
                     EHWYSADLQMTVMSKRSDPRFGDTAYTLTNIQRQEPAASLFAVPSDYTVTTGKNRMFI
                     RKFNGGPDAPPLPPPDN"
     gene            complement(2792..2959)
                     /locus_tag="VGR03_04115"
     CDS             complement(2792..2959)
                     /locus_tag="VGR03_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993496.1"
                     /translation="MGAAAFWERWKPADGTYLKEHEGPVGTWIAQLKKGLAAIAAAKI
                     SQQYGREEEKE"
     gene            complement(3012..>3495)
                     /locus_tag="VGR03_04120"
     CDS             complement(3012..>3495)
                     /locus_tag="VGR03_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993497.1"
                     /translation="TQHGTLKMRRTAVVAAWALAAAAVLVGAVGWRNWRHSQHEEAAN
                     HPSSIPAARNLPENNSAAGANHQTAAEVPNGMQQRGSAGAATSGTLVADNELSGFTFL
                     PGSFAADTDDAAILRVRMQRGALGVLGLPVNEERASDWIQVDLLVGDDGLPQAVRLPK
                     "
BASE COUNT          647 a         1234 c          852 g          762 t
ORIGIN      
        1 ttctcccgtg ccccgccacc catgctcgcc cgccacgcgc tccttctaac tcattcaata
       61 ttttcaggcc cggccctgtt aatttctgag aaagataccg cctccgtaag tcctttagct
      121 gcaacactta tggctttccc cgcaagtgtt gcaaacaaaa gacttacagc gagtctaact
      181 cttttagatg caacacttac gaaaaacagg gggtggggga agagggccgc gtttcctcga
      241 cgttccgatc aaagctccac cgcagttgtg gagcggagat cccgacccgg tcgggacgct
      301 cgctcaatcc ttacttcctt acctcattac ctccatactt ccttacctcg ttgcttccac
      361 cagtcacccg tgatcactca agccgccctt ccgaaccggc cctgtcggcc ttatcatcgt
      421 tcctcacacc aaaccatgtc cctgccgcct atccccggcc tcattcccgt ctacgaggac
      481 tacgcccgct gcatccactg cggcctctgc ctcaacgcct gtcccaccta ccgcctctgg
      541 aacctcgaag ccgattcgcc acgcggccgc atccatcaaa tgatccacgt cgagttggac
      601 aagcaaccca tcaccgattc cttcgtcgat cacatcgaca aatgcctcga ctgccgcgcc
      661 tgcgagacca cctgcccctc cggcgtcgaa tatggaaaac tcgtcgagca cgcccgtgca
      721 cgcatcgagc gcgattatcc ccgctcctgg ctcgcccggg tcacccgcga ctttgtcttc
      781 cggcacttgc tccccgatcc acatcgcatc atcgacttcg cgcgcgttat ccgtttctac
      841 cagcgttccg gcatgcagac catcgcgcgc ggcatcggga tactaaaact actcggtctc
      901 gccgagcgcg aacgcctcct tccgcgcatc gacgacgatt ttttctaccg ccgtctcggc
      961 tggacgttcc ccgccgccgg tccgcgccgc gcccgcgtcg ccttcttcgc cggatgcgtc
     1021 gccaacgtca ctttttctca gctcaacgaa gccaccatcc gcgtcctcac cgccaacggc
     1081 tgcgaagttg tcgttcccag cggccaactc tgctgcggcg cgcttgccgc gcacgcgggc
     1141 gttcgcgatg ccgcccgcga cctcgcgcga aagaatctct ccgtatttct ccgcgaagac
     1201 ttcgacgcca ttgtcaccaa cgccgccggc tgcggctcca cgctgaagga atacgatcat
     1261 ctcttctcgc aagacgagga agagcacaac caggcccggg tctttgccgc caagactcgc
     1321 gacgtcaccg aatttctcgc cgctctcggg ctcaccgcga aattgaagcc gcttccgctg
     1381 cgcgtcacct accaggattc ctgccatctt catcacggcc aaaaaatccg cgaagctccg
     1441 cgcatgctcc tccgcgccat tcccaatctc gattttgtgg aattgcccta ctcggaaatc
     1501 tgctgcggct ccgccggcgt ctacaacgtc acgcaaaccg aaacatccct cgaattactc
     1561 gccgagaaaa tgcgccacgc ccaatccact cgcgcccaaa ccatcgtcac cgccaacccc
     1621 ggctgtctgt tgcaactgcg cgccggcgct gccatccacc gcaccaacca ggaggtcctc
     1681 cacgtcgtcg aactcctcga ccgcgccatg caaccatgaa aacgcggaac gctggcccac
     1741 acgcgcgctg tgtttcgtta gttgattatt tctttacttc atcttttcct ggtctccaaa
     1801 cacggctgcg ccctttccgc agcgatgggg gcatctgcga aaagggcgtg ccgtcttcag
     1861 gagagaagat cgtgccgact actctgcagt cgccgagacc aatgatgaaa tcagttgtcg
     1921 ggaggcggca gcggaggtgc gtcgggcccg ccattgaact tccgaatgaa cattctgttt
     1981 ttgccggtcg ttaccgtgta gtccgatggc acggcgaaga gcgaagcggc cggttcttgc
     2041 cgctgaatat tcgtcagcgt gtaggccgta tcgccaaaac gcggatcgct gcgcttgctc
     2101 atgaccgtca tttgcagatc ggcggagtac caatgctcgg atacgatggt gatcggtttc
     2161 tcgtttccca tctgtcccgc gggaatcgta tgggtgatgc gcgttccttg tgcgctcacg
     2221 ccagcgatcg tctgcgcacc caggtcttcc tttttcagcg agccgtcacc gatggcctgc
     2281 tgcacgtgat attccatctt gccctttatc acgtccttca tcttgccaaa tggcatctcc
     2341 attttttccg ccgttttttc gtcggggtgc aagatgaaat tagtgcccgc cacgggatca
     2401 ttgatgaaca caaacgactt cggctgtccc gaggtcgcca gcggcccgat cgcaggcaac
     2461 gtcacttcct tgcggaaacg tccctggcta tcccgataga gattcgtctc cgtctttcgc
     2521 gcgatatggt ttccgtccgc gagagtttgc gtcgactccg aaactgcgac cgcgctgaac
     2581 ggcacgccgg ttacgacctt tccgccgtgc attccctcga aacccagcaa ctcgatgcgt
     2641 tcgccgatca tgccagcttc cggtcccgga ccttgcgcca tgacgaccgg gcccggaggc
     2701 aatggcgcct ctggctcctg cgctcgcact actcctgcat tcagcaagag agcgccactc
     2761 aaaacggaat acaagtacat cttgcgattc attattcctt ctcctcttct ctcccgtact
     2821 gttgcgaaat ttttgctgct gcaatcgcgg ccagcccctt tttcaattgc gcgatccatg
     2881 ttccgaccgg cccttcatgt tccttcagat atgttccatc cgccggcttc catctctccc
     2941 aaaaggccgc tgcacccatg cgtgtgttcc tccccgaggc gctgcgagag cgccccgctc
     3001 cccacgaaca tttatttcgg cagccgcacc gcttgcggca atccatcatc gccgacgagc
     3061 aaatccacct gaatccagtc gctggctcgc tcttcgttaa caggcagtcc cagcacaccc
     3121 agcgcgcccc gttgcatgcg cacccgcaga atcgcggcgt catcggtatc cgcggcgaag
     3181 gatccaggca gaaacgtgaa gccgctcagc tcattgtcag caacaagtgt tccggaagtg
     3241 gcagctcctg ccgaaccccg ttgctgcatt ccatttggaa cctcggcagc agtttgatga
     3301 ttcgctccgg cggcggaatt gttttccggc aaattgcgcg cggccgggat ggaactcgga
     3361 tggttcgcag cctcttcgtg ctgactgtgg cgccaattgc gccagcccac cgcgccgaca
     3421 agcacggccg cagccgccag cgcccacgca gcaaccacgg ccgtgcgccg cattttcaat
     3481 gtgccgtgct gcgtg
//
LOCUS       DASYVO010000126         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_243130, whole genome shotgun sequence.
ACCESSION   DASYVO010000126 DASYVO010000000
VERSION     DASYVO010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243130"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1448)
                     /locus_tag="VGR03_04125"
     CDS             complement(21..1448)
                     /locus_tag="VGR03_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV1993498.1"
                     /translation="MRNRGISRRDYFKLLGTGAAATAGAGNLFVNGSATIAAQGRPEP
                     QPVESPEVVANRARRMKWWHDAKFGMFIHWGLYSVIGQHEWAMEREGIPVAEYELLAK
                     HFVPQPHAARAWARLAKQAGQKYMVMTTKHHEGFCNFDTKLTDYCAPRQACGRDLVAE
                     FVEAARAEGLGVGFYYSLMDWHHPDGARCTTDEAARQRFVEYTHGLIRELMTNYGKVD
                     VLWYDVSWPLDARGWESEKMNEMVFKLQPDIIVNNRNRLPGDFSTPEQRIVAESGGRA
                     WEACMTLNDSWGYQKADDDWKSPRTVLRNLATCAKGGGNYLLNIGPRADGSIPEESVR
                     ILREVGKWMEKNGPTIYQSQPCEAGGVPMANFTRKGNTLYIHVHYWPGETPASQWLSF
                     FRPSSVVAIGGLRARVKSARLFASGKKVDFRQDDVSTQFVGLPAAAPDSPVTVIEVEC
                     DSEPRVEGAYVREHRPRAKVGVNWS"
     gene            2175..2576
                     /locus_tag="VGR03_04130"
     CDS             2175..2576
                     /locus_tag="VGR03_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEV1993499.1"
                     /translation="MGKALAAFLPAAQRERALPGLVFQPVTRRTIRNSAEFQKELLKI
                     HRRGYALDNEESVPGARCVAAPIVNARNEAVAAISVSGPVARITNDKIPMFATAIKKA
                     VRTIAEQMGFTGQPPGRVRAGRNEGRRAGDY"
BASE COUNT          559 a          822 c          793 g          618 t
ORIGIN      
        1 gtgaataccc gtgctaggtg ctaagaccag tttactccaa ctttggccct gggccggtgt
       61 tcacggacgt aggcgccttc gacgcgcggt tccgagtcgc attccacttc gatcaccgtc
      121 accggactat cgggagccgc cgccggcagg cccacgaatt gtgttgagac atcgtcctgg
      181 cggaagtcta ctttcttgcc ggaagcgaac agccgcgctg acttcaccct cgcgcgcagt
      241 ccgccgatag cgaccacgct tgaaggccgg aagaacgaaa gccactggga ggcaggtgtt
      301 tccccgggcc agtaatgcac gtgaatgtaa agcgtattcc ccttgcgagt aaaattcgcc
      361 atcggcactc cgccggcttc gcagggctgg gattggtaaa tggtagggcc gttcttttcc
      421 atccactttc ccacttcgcg cagaatgcga actgactcct ccggaatgga accgtcggcc
      481 cgggggccga tatttaacag atagtttccg ccgcctttgg cgcaggtagc caggttcctc
      541 aacaccgtgc gcggtgattt ccaatcgtcg tccgccttct ggtatcccca actgtcgttc
      601 agggtcatgc aagcttccca cgcccggccg ccgctttcgg caacgatgcg ctgctccggg
      661 gtggagaaat ctcccggaag acggtttctg ttgttcacga tgatgtccgg ctggagcttg
      721 aaaaccattt cattcatctt ctcggattcc cacccgcggg cgtcgagcgg ccaggaaacg
      781 tcgtaccaca gaacgtccac cttgccgtag ttggtcatca gctcccgaat caggccgtgc
      841 gtgtactcga cgaagcgctg gcgcgccgct tcatcggtgg tgcaacgggc gccgtcggga
      901 tggtgccaat ccatcagcga ataatagaac cctacgccca ggccctcggc gcgcgccgcc
      961 tccacgaatt ccgctaccag atcgcggcca caggcttgcc gcggagcgca gtaatcggtt
     1021 aatttcgtgt cgaaattgca gaagccttcg tggtgcttgg tggtcatcac catgtatttt
     1081 tgccccgcct gcttggcgag ccgggcccag gcgcgcgcgg catgcggctg gggaacaaaa
     1141 tgcttggcca gcaattcgta ctctgccacc ggaatgcctt ctcgttccat cgcccactcg
     1201 tgctggccga tgacgctgta caatccccag tgaataaaca ttccgaactt ggcgtcatgc
     1261 caccacttca tgcgccgcgc gcgattcgcc accacctccg ggctttccac gggctgcggc
     1321 tcgggccgcc cttgggcggc aatcgtcgcg cttccgttaa cgaataagtt ccccgctccc
     1381 gccgttgctg cggctcctgt cccaagcagc ttaaaataat cgcgacgaga tatccccctg
     1441 ttgcgcatgt gttcctccct tttatcaact ggcgctgtag ctagcttaag gaccggcgag
     1501 gatcgtgtag atcccaaggt ttggcctgcc tgaggcgaac agctttcacc ttcttaccaa
     1561 ccttgtcaag aaaaacgtta gaatatgaaa gatgatttca caatctaaaa tgaccgtttg
     1621 tggacgcccg tgcagttcgg tatggtagcg gcgagtttac ctcgccacgt agcggtgtaa
     1681 agccggcgtc cgctcgaaac gcattacgcc gcgcaatttg tcgcagaatt tttaaggagg
     1741 gtctttgagc ccggagagaa ttttgaaagg agccaccgtc ggagtgttaa caaaagtttt
     1801 gaagatcttc gagctccttc aaaaccagcc cacaggtgtg gagttgaagg atatcagctt
     1861 tgaaacggga atccacaaaa gcaccgctta ccggttcctc tcgcacctgg agcgcgaaag
     1921 ttacgtgaag cgcaacggta cgggaggcta cgtcatcggc gtcaagtttt tgtcgctttg
     1981 cagcgggatg gaccatcgga cggtgctgcg tgaaacggcg ctgccgacac tgcgggaact
     2041 tcagcaggtt acccaggaga ctgtgaatct cggggttctg gatgacggtc aggtcttata
     2101 catcgaagtc ctggaaagtc cacacgtgtt tcgtctggtc tcgcaggtag gagcgcaccg
     2161 accactccac tcgcctggga aaggcgctcg cggctttctt gcccgccgct cagagagaac
     2221 gtgccttacc tgggttggtg tttcagcctg tcacgcggcg cactattcgc aactccgccg
     2281 agttccagaa agagttgcta aaaattcatc gtcgaggata tgccctggac aacgaagaga
     2341 gtgtacccgg tgcccgttgc gtcgctgcgc ccatcgtcaa cgcgcgcaac gaggccgtgg
     2401 ccgcgattag tgtgtccggt ccggtggcaa gaatcaccaa cgataagatt cccatgtttg
     2461 cgacggccat caagaaagcc gtccgcacaa ttgcggagca aatgggattt acaggtcaac
     2521 cgccgggccg ggtgcgggca ggcaggaacg aaggccggag ggccggcgat tattagagta
     2581 cgaacttcaa tttctctcct ctggtttttt accgtatgcg ggagcggcgt cgcgctctgt
     2641 ttcgctagtg cactctgcgc gacaaatgca ttgaaggtca cggggagtcg ctttcggctc
     2701 gtcgaatcag ttcccgcatg gtggagtcct gcgggaaaac ttcgagctcc tttttcatca
     2761 ccgcgagcgc ctctgcgtaa tggtgcgttt tc
//
LOCUS       DASYVO010000127         9701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_172346, whole genome shotgun sequence.
ACCESSION   DASYVO010000127 DASYVO010000000
VERSION     DASYVO010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9701
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172346"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..1122)
                     /locus_tag="VGR03_04135"
     CDS             complement(226..1122)
                     /locus_tag="VGR03_04135"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1993500.1"
                     /translation="MNSKQVVLITGSSTGFGRLFAETLARKGHTVFATMRDPVGRNAK
                     NASEIRTLAEKESLPIHVLELDVSDDASVGRAVDAAVAKAGRIDVAINNAGYYLSGLA
                     EAVTTEQAQRLMDTNFLGPVRVNRAVLPHMRRQRSGMLMHISSGAGRVILPSAGFYCA
                     SKFALEALAEAYSYELAAQGIESVIVEPGQYETPVFGNTVTAADQARTNTYGAAKEFP
                     AKINGALSSTAGNAQEVADAVLRIIETPAGERQLRYFVSPQNFGVEEINALSRQVQAN
                     VLEAFGLAADTKFLKGKAAGSV"
     gene            complement(1592..2722)
                     /locus_tag="VGR03_04140"
     CDS             complement(1592..2722)
                     /locus_tag="VGR03_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993501.1"
                     /translation="MKLSRSATLAVLVGFCLCLFAQVAVAQVPPPSMPCSGANLVDQK
                     FPTVGPEVTHWRLCWQAVNGNGLKIHWAYFRTSPAKPWLFLLWDAEVSEIFVPYHTGS
                     PRFYDVGYMFPLTPVGAADCPASQGGTVLAGNVCLQVHDRGLSWKSDSQVYRGEEIVL
                     WGALAAANYNYIIEWTFRDDGIVMGRVGATSHNFPGREFETHVHNPIWRLDVDLNGWP
                     NDSVALGTHTEVGLTGNDTQPPIPLSTGLLWDPRAFNTLDVSDKFLVNGKGDKTYYQL
                     IPLPTQGLAQHQEAFTKPDFWVTHYNPAQMSAKDLISYTNPPVSVSDTDIVIWYRGSV
                     HHHPRDEDGEKVGGAWHGVALVMWTGFMLKPHDLFDRTPFYP"
     gene            complement(2724..3470)
                     /locus_tag="VGR03_04145"
     CDS             complement(2724..3470)
                     /locus_tag="VGR03_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993502.1"
                     /translation="MQTHRWSLMRIINRIAFSILALLSFIFPAAIASWAQNTQPGSRV
                     ATDRRALDPLTPAEQALAERIARSDGRVKEVLGEAGVRLVSVIPVLLKADVPEKTNVA
                     QRQVEVVLFQPQREVGARVIVNLRQNVAVAVNRLTGDQVPYTNDDLADAFQLALRDPE
                     VVRVLGPAAQTFRPRTGPPTTTAATPENVVEGLPVRSSDPNDPCSKHRCLWLMFRRGN
                     DYLSQPSVTVDLTDKRVSVEKPVTDRKADH"
     gene            complement(3793..4134)
                     /locus_tag="VGR03_04150"
     CDS             complement(3793..4134)
                     /locus_tag="VGR03_04150"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEV1993503.1"
                     /translation="MSDLAAKACVPCRGGVPPLKGEELASLEKQVNGWKVMEGHHLAK
                     TLKFPDFREALKFVNRVGELAEEQGHHPDIFLAWGRVEITIWTHKINGLTESDFILAA
                     KIDQLATPAQG"
     gene            4567..4884
                     /locus_tag="VGR03_04155"
     CDS             4567..4884
                     /locus_tag="VGR03_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEV1993504.1"
                     /translation="MTYIIAEPCIGTKDTACVDVCPVDCIHPAKGRTYEDGRPAYDEV
                     PILYIDPVECIDCGACVPVCPVTAIFAEPDLPEKWNAYIEINASYVVGGKFQPDKYKK
                     PSS"
     gene            5058..6599
                     /locus_tag="VGR03_04160"
     CDS             5058..6599
                     /locus_tag="VGR03_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEV1993505.1"
                     /translation="MTDNRNPEAAWSYHNATKHSYASIRAHAHSLDWANQPLPFKIYP
                     TLELMRLPGEVRQTGVAALSAIAESVPAQTNAAPDLEAVAQLLYLSAGITRKRNYPGG
                     EIYFRAAACTGALYEVELYLVCSDLENLPAGVYHFAAAEFGLRRLRAGDYRRVLLEAT
                     GDEPAIAHAPLTIVCTGTYWRNAWKYQARTYRHFGWDNGTLLANLLAAGTALGLPARV
                     VCGFVDADVSRLLDVDPQREVAISLVALGRVAALPPQTPAEISPLSLESVPLSRREVD
                     YPLMREMHAASSLDSAEEAEKWRGHTPMTESPAAAGAIVPLRPLSDAEIPRDPIEQVI
                     LRRGSSRKFVRAPISLAQLSTMLDRASRGVRADFLDPPGTQLNHLYLIVHAVDELPPG
                     AYVFHRNRGVLECLKQGNFRDQAGYLGLEQELPADAVADIFFLADLRPILQRFGNRGY
                     RAVQLEAGILGGKLYLAAYAQRLGATGLTFYDDDVIRFFSPHADGKSAIFLVAVGKSA
                     KDRSE"
     gene            complement(7175..7396)
                     /locus_tag="VGR03_04165"
     CDS             complement(7175..7396)
                     /locus_tag="VGR03_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993506.1"
                     /translation="MTLVEVTYELQSPLGTEELRRLGEFANTYGLRRFRVDDANRQIS
                     FEYDASRLRETEVAHALRLARIAVTRKVN"
     gene            complement(7505..8047)
                     /locus_tag="VGR03_04170"
     CDS             complement(7505..8047)
                     /locus_tag="VGR03_04170"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV1993507.1"
                     /translation="MDIPRKLFAWGGLAVALLLLFAFLHPLYRQGEASVAGNTAPDFS
                     LTLANKPIHLSDLRGKVVVLNFWATWCPPCVEETPALNRLQQRIASRGGVVLGVSADS
                     DPALYEKFLKDQKVEFPTYRDPDLKGNLSQIALKYGTSMYPETYIIDSRGKIARKVIG
                     PQEWDSPEMVAYLDSLLGKG"
     gene            8110..9204
                     /locus_tag="VGR03_04175"
     CDS             8110..9204
                     /locus_tag="VGR03_04175"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1993508.1"
                     /translation="MSSHAFNQSVAEALPLAEAAAKVSPQISAIVPARDEEEVIAACI
                     ESLAGQPEIGEIIVVNDASTDGTGEILRRLQEAIPMLRVVESAGIPAGWVGKNYAAWL
                     GAAEARLTWMLFTDADSRHLACSAGRALRLAEEHGAALVSYSPGQVTRTWWEKALIPF
                     IYCRLAARFSYEAVNSPASAAAAANGQYLLIRRTAYATVGGHQSVAGEILEDVALAER
                     VKAAGLRLWFGPGAEAVEVRMYRTFRAMWEGWKKNLYRLMGNSAGGARRELMATVPWI
                     PCLLLLGGFVRPVLALVGVGLLLGRHAAYAAALSRNQFPVSRIVFYMPAVALYAAVLL
                     ASWRSHAEGRVRWKGREYAVGSPAAEGEGR"
     gene            9204..9626
                     /locus_tag="VGR03_04180"
     CDS             9204..9626
                     /locus_tag="VGR03_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEV1993509.1"
                     /translation="MVTITRKIEFAASHRYNNPALSAEENRRIFGKCNNPHGHGHNYI
                     LEVTVAGEPDPMTGMVLDLKELKELLEKEVMQRMDHRHLNHEVPELAGKIPTCENIAQ
                     VIWELLEPKITRGRLHRIRLHESADLFADCYRNGASGA"
BASE COUNT         1919 a         2788 c         3086 g         1908 t
ORIGIN      
        1 gcggattcta aaggacttac gaagcgattt ctcggaagtg cggattctaa aggggttgag
       61 ggggaccgag ggacttggcc gatcattcac gccgcattat atacgagaag taaacagatg
      121 tcaagggatt tctgcacttc cccacgcttc ccccgcgaag catggggcac ccggcaaatc
      181 tttttgtcga cggatccttc cagaagctgt tcctgcaaga gagagctaga cggagccggc
      241 tgctttacct ttgaggaatt tagtgtcggc agcgagaccg aaggcttcta gcacgttggc
      301 ttgcacttgc cttgacaggg cgttgatctc ctcaacaccg aagttctggg gactgacgaa
      361 atacctcaat tgcctctctc cggcaggagt ctcgataatc cgcagaacgg cgtccgcgac
      421 ttcctgggca ttgccggccg tggaggaaag cgccccgttg atcttcgcag ggaactcctt
      481 ggcagcgcca tacgtattgg tgcgagcctg gtcggcagcc gtcactgtat tgccgaaaac
      541 aggcgtctca tattggccgg gttcgacaat cacggattca attccttgcg cggcaagttc
      601 gtagctgtac gcctcggcca gcgcctcgag tgcaaatttg ctggcgcaat agaaacccgc
      661 ggaaggaaga atcacacggc cggcgccgct gctgatgtgc atgagcatgc cgctgcgttg
      721 gcgccgcatg tgcgggagca ccgcgcgatt tacgcgcacc gggccaagga aattcgtgtc
      781 catcagacgc tgcgcttgtt cggtggtgac cgcttccgcc agccccgaga ggtagtagcc
      841 ggcattgttg atggcgacat cgatgcggcc cgctttggcg acggcggcgt ccacagcccg
      901 ccccaccgag gcgtcgtcgc ttacgtccag ttcaaggacg tggattggca gtgactcctt
      961 ttccgcaagc gtgcggattt cggaagcatt cttggcattg cgacctacag ggtcacgcat
     1021 cgtagcgaaa acagtgtgcc ctttgcgtgc cagggtttcc gcgaaaagtc tgccgaagcc
     1081 agtgctggag ccggtgatga gaaccacttg cttggaattc atggttatcc ttccttgcct
     1141 gttgtggcga taccggacac tttgatgccg gaagcaaagc gtttgggtac aagaaatgaa
     1201 ttcagcaaat tgactaccag agagaatgaa acggcgcgta cgtaagtgat ctatgggcca
     1261 cagggcgcgc ttgtcctata tctactgcat ctcgaaacag tctccgaacg agccttattc
     1321 tgacagtttg cgggcgagtt gtcggcaact cggaaactat ctcaacgcct tgtggttcct
     1381 attctgacgg tggcggccac ttggctgtgt agttggatta ccggacaagt ggttcatcgc
     1441 catccggatt cacaaacgtg gatctgggaa accggccacc cgccactcta cccgcgcaaa
     1501 cggctggaca gcgccaagtg atagtcgggt aatccccacc cttacataca ccgaagaatg
     1561 ggccacccgc ccctcttgcc ggtgaatcac ctcatggata gaacggcgtg cgatcgaaaa
     1621 ggtcatgtgg cttgagcatg aaaccagtcc acatcaccag ggccacgccg tgccaggcgc
     1681 cgcccacttt ttcgccgtct tcgtcgcggg gatgatgatg gaccgagccc cggtaccaga
     1741 tgacgatgtc ggtgtcgctg actgacacgg gaggattggt ataggagatc aagtccttgg
     1801 cgctcatttg cgcggggttg tagtgcgtga cccagaagtc gggcttggtg aacgcctcct
     1861 ggtgctgggc caggccctgg gtgggcagcg ggatcaactg atagtaggtc ttgtcgccct
     1921 tgccgttgac caggaacttg tcggagacgt cgagcgtgtt gaaggcgcgc ggatcccaca
     1981 gcaggcccgt ggacaaggga atgggcggct gggtgtcgtt gcctgtgagt cccacttctg
     2041 tgtgagtgcc cagagcgacg gagtcgttcg gccatccgtt cagatccacg tccagccgcc
     2101 agatgggatt atggacgtga gtttcgaatt cacgtccggg gaagttgtgc gacgtggcgc
     2161 cgacccgtcc catgacgatg ccgtcgtcac ggaacgtcca ttcgatgatg tagttgtagt
     2221 tggcggccgc gagcgccccc cacaggacaa tctcttcgcc gcgatagacc tgggagtcgg
     2281 atttccagga aagcccgcga tcgtggactt gcaggcacac gttgccggcc agcacggttc
     2341 cgccctggct ggccgggcag tcggccgcgc cgaccggggt gagcgggaac atgtaaccga
     2401 cgtcatagaa gcgcggcgat cccgtatggt agggcacgaa gatttcagaa acctccgcgt
     2461 cccagagcag gaaaagccag ggcttcgcag gcgaggtgcg gaagtaggcc caatgaatct
     2521 tcaggccgtt gccgttcacc gcctgccagc acagccgcca atgcgtgacc tccggaccca
     2581 cggtggggaa cttctggtcc accaggttcg cgccggagca gggcatggag ggcggcggca
     2641 cctgcgccac agcgacctgg gcgaacaggc agagacagaa tcccacaagg acagcaagag
     2701 tagcgcttcg cgaaagtttc atattagtgg tccgcctttc tgtcggtgac cggcttttcg
     2761 acagatacac gcttgtccgt gagatctaca gtcacactcg gttggctcag atagtcgttg
     2821 ccacggcgga acatcagcca caggcagcgg tgtttcgaac acgggtcgtt ggggtcgctg
     2881 gagcgaaccg gcaatccctc cacgacattc tccggagttg ccgcggtcgt ggttggcggc
     2941 ccggtccgcg gccggaacgt ctgcgcggcc gggcccagca cgcgcaccac ttcaggatcg
     3001 cgcaaggcca gttgaaacgc gtcggctagg tcatcattgg tgtatggaac ctggtcgccg
     3061 gtcaggcggt ttaccgccac cgcgacgttc tggcggagat tgacgatgac ccgtgcaccc
     3121 acctcgcgct ggggttgaaa cagcacaact tcaacttgcc gctgcgccac gttggttttc
     3181 tcggggacgt cggccttgag caagacagga atgaccgaaa ccaggcgcac gccggcttcg
     3241 ccgagaacct ctttaactct gccgtcactc cgggcaatac gctcggccag agcttgctcg
     3301 gccggcgtca aaggatcgag cgcgcgccga tccgtggcaa ccctcgaccc cggctgcgtg
     3361 ttctgtgccc atgaagcgat ggcagcggga aatataaaac tcaaaagggc aagaatcgaa
     3421 aaggcgattc tgttgatgat ccgcatgagc gaccaacgat gagtttgcaa agaacacctc
     3481 catcaggggg aacaaatctg ctgattagca ggatgaacgg tccccttcca gaacgtcctg
     3541 cacccgctca agcagctgcg tgacacaatg ccggtctcct tgtcagtgct ttccgggttc
     3601 ggaattaact gcatacatga agttccttga gctggggaaa aacggggcct ctcagtgctt
     3661 ggaataatta ctcctctatc ccgacattga ggctgtattc tgctactcgc tcactgggct
     3721 gtcaagagaa tcttcagact cactcggact gcgttcgacc gcgtgaagca actcccggga
     3781 gtcctcggag tgtcatcctt gcgcgggggt cgcgagctgg tcgatctttg cggccaggat
     3841 gaaatcgctc tccgtcaagc cattgatttt gtgcgtccag atggtgatct ccactcttcc
     3901 ccaggccagg aagatgtcag gatggtggcc ttgctcttcc gcgagctcgc caacccggtt
     3961 gacgaatttg agagcctcgc gaaagtcggg aaacttcagg gtttttgcaa ggtggtgtcc
     4021 ctccatcacc ttccatccat tcacctgctt ttccagagag gcgagttcct caccctttag
     4081 cggtggtaca ccgccacggc atgggacgca ggctttggct gcgaggtctg acatcacaac
     4141 cactattcta gtgcgctgtc ccgcaaattg ctaacggagc ctgttggcct caggggccct
     4201 tcgtttctca gggcgagctg aagcccagtt tgtttttcag ccgtttatgt cggagctcct
     4261 ttccgccggg agcggaatct acgatcggct gcgctcagga caagtgaggc tccgaccccc
     4321 tagagagaaa tgcagcgaat ttctgggaca cgacatttgg aggtttgggg tttgagctgg
     4381 ttcaggatga agacccggaa aatggggaca gtgtgggacg cgaaaactgc ggggtcttgg
     4441 ggtgacgagg ggctcgatgg ggactttgac agcggttgag tttgaggagt accgttcgga
     4501 gagaacggca ggaacatgtt tccgtgcctt ttgaaggctg gcgcggtcaa cgaagggagg
     4561 tgaccgttga cctacatcat cgccgaaccc tgtatcggga ccaaagatac cgcctgtgtg
     4621 gacgtgtgcc cggtggattg tatccatccg gcaaagggcc gaacctacga agatggccgg
     4681 ccagcgtacg acgaggtgcc catactgtac atcgaccctg tcgagtgcat tgactgcggg
     4741 gcctgtgtgc cggtgtgtcc ggtgacggcc atcttcgcag aacccgatct gccggaaaag
     4801 tggaacgcgt atatcgagat caacgcgagc tacgtcgttg gcggcaagtt tcagcccgat
     4861 aaatataaga agccaagtag ctgacggccg cctctgcacg acacccggca cgtctgagtg
     4921 cgctgcgaag atggccacct catccttcgg ctgcgctcag gataaaaggt ggccgctaca
     4981 ataagaggga aagaggctga agctgtgggc cagccgcccc ggggaatgac cttccgtatc
     5041 gttcgttcat tgccactatg accgacaacc gcaatccaga agccgcttgg agctatcaca
     5101 acgccaccaa gcactcctac gccagcattc gcgcccacgc ccattccctg gactgggcga
     5161 accagccgct gcccttcaag atttatccga cgctggaact catgcgcttg cccggcgagg
     5221 tgcggcaaac cggggtcgcc gccctctctg ccatcgccga gagcgtgccc gcccagacaa
     5281 acgcggcccc agacctggaa gcggtggcgc aactcctgta cctttccgcc ggcatcaccc
     5341 gcaagaggaa ctatccgggc ggcgagatct actttcgcgc cgcggcctgc accggagcgc
     5401 tctacgaggt cgagctttac ctggtttgca gcgacctcga gaacctgccg gccggcgtct
     5461 atcacttcgc cgccgccgag ttcggcttgc gcagactgcg cgccggggat tatcgccgcg
     5521 tgctgctcga agccaccggc gatgaacccg ccatcgctca tgcgccgctg accatcgtct
     5581 gcaccggcac ttactggcgc aatgcgtgga aatatcaggc gcgcacgtac cggcatttcg
     5641 gctgggacaa cggcactctc ctcgcaaacc tgctggccgc cggcacggca ttgggtctgc
     5701 cagcccgggt ggtatgcggc ttcgtggacg cggacgtcag ccgcctgctg gatgtggatc
     5761 cccagcgtga ggtggccatc tccctggtgg ccctcggccg cgtcgcagct cttccgccgc
     5821 aaacccctgc ggaaatctct ccgctctccc tggaaagcgt cccgctctcc cggcgggagg
     5881 ttgattatcc gctcatgcgc gaaatgcacg cggcgtcttc gctcgattcg gcggaggaag
     5941 cggagaagtg gcgaggccac acgccgatga cagaatctcc ggcggccgcg ggagcaatcg
     6001 ttccgctccg gcctttgtcc gacgcggaga taccacgcga ccccatcgaa caggtgatcc
     6061 tgcgacgcgg ttccagccgg aaatttgtcc gcgcgccgat cagtctggcg caactttcga
     6121 ccatgctgga ccgagcgagc cgtggggtga gggcggattt cctcgatccg ccgggaactc
     6181 agctcaatca cctctacctg atcgtccatg cagttgatga actccctccc ggagcctatg
     6241 tcttccaccg caaccggggc gtgctggagt gcctgaagca ggggaatttc cgcgaccagg
     6301 ccggctacct gggactggag caggagctgc cagccgacgc cgtcgcggac atatttttcc
     6361 ttgctgattt gcggcccatt ttgcagcgct ttggcaatcg cggctatcgc gccgtgcagc
     6421 tcgaggccgg tattctgggc ggcaagctgt acttggcggc ctatgcgcag cggctgggcg
     6481 ccaccgggct cactttctac gacgacgacg tgatccgctt cttctctcct catgctgacg
     6541 gcaagagcgc cattttcctg gtggccgtgg gcaagagcgc gaaagacaga tccgagtgaa
     6601 cggtcaggcc ggcgcagaga aggcttaagt tctctgagat cgaaaccgac cggtcggggc
     6661 gattggcagg gagtggcgga tggtggctac cgcgatagtg ggaatacgct gggaaaagaa
     6721 agtgaaggga agaggagttc cgaatgagaa cattccgcag tcagagttcc gaattgaact
     6781 ttcatgacca atagggatgt gatcttggtt gcatgggctg tggataagtt gtgggaattg
     6841 aggaagttag cagatggagc cagaagacga atttggccca ggtcgtgctg ttttgtggcg
     6901 cgtgggcgcg tttcgattga cgacttggac ggagtgtcga gtgaggggga aggggcttgc
     6961 tcgccattct gaacaggtcg ttcccacctg cctctgagag ggaagctagg atggatgacc
     7021 ccacgctccg gtgtgaaaac gatgccggaa acggaacgcg cctttcgggg ctcccggacc
     7081 caagcgggag ccccgctctc tttttagccg gctaaaccgg taactatttc accgctctcc
     7141 gctaccgggc ctcactcacc tgagattttt ccgatcagtt gacctttcgc gtgacggcga
     7201 tgcgcgccag gcggagcgca tgcgcgacct ccgtttcgcg caagcgcgaa gcatcgtatt
     7261 cgaaagagat ctggcggttg gcatcgtcca cgcggaaacg ccgcaagccg taggtattgg
     7321 cgaattctcc gaggcggcgg agttcttctg ttccgagcgg ggactgcagt tcgtaagtga
     7381 cttcgacaag ggtcatggag gaattatagc agccgggggc aaagccgggc cgggcgcggc
     7441 gcggcgggcg gagccgtcga gggacttgcg tatctgcggc aatggctttt tccgagggtg
     7501 ttgctcagcc cttgccgaga agcgagtcga ggtaagcgac catctcggga gagtcccact
     7561 cctgggggcc gatgaccttg cgcgcgatct tgccgcggct gtcaatgata taggtttccg
     7621 gatacatgga agtgccgtac ttcagggcaa tttgggaaag attccctttc aggtcgggat
     7681 cccgataggt cggaaattca accttttgat ccttcaagaa tttttcgtag agtgccggat
     7741 ctgagtcggc gctcacaccc aggacaacgc cgccacgtga ggcgatgcgc tgctgcagac
     7801 ggttcagcgc gggggtttcc tcgacgcacg gggggcacca cgtcgcccag aaattcagca
     7861 ccacgacctt gccgcgcaga tccgagaggt ggatgggctt gttcgcgagc gtcagcgaaa
     7921 agtccggggc ggtattgccg gccaccgatg cctcgccctg gcgatacaag gggtggagga
     7981 aagcgaacag caggagcagc gcaacggcaa ggccgcccca ggcgaacagt ttccgaggga
     8041 tgtccatcag atgctataat accgtttccg ccccggaccg cgaaacgctc cgcgctcccc
     8101 ccaacagaga tgagcagcca cgctttcaat cagtcggtcg ccgaggcgct tcctcttgcc
     8161 gaggccgccg ctaaggtttc cccgcaaatc agcgccatcg tgcccgcgcg cgacgaagaa
     8221 gaggtcatcg cggcctgcat cgagtcgctc gccgggcagc cggaaatcgg cgaaatcatc
     8281 gttgtgaacg atgcttcgac ggacggaaca ggagaaatcc tgcgacggct ccaggaagca
     8341 attcccatgc tgcgcgtcgt ggaatccgcg ggaattccgg cgggctgggt ggggaagaat
     8401 tacgcggcct ggctcggggc ggcggaggct cggctgacgt ggatgctatt taccgacgcg
     8461 gattcgcggc atctcgcgtg ctcagcgggg cgggcgctgc gcctggccga agaacacggc
     8521 gccgccctgg tctcttattc acccgggcag gtgacgcgca cctggtggga gaaggcgctg
     8581 attccgttta tctattgccg tctggcggcg cgattctctt atgaagcggt gaacagtccg
     8641 gcatcggctg cggccgcggc gaacgggcaa tacctgctga tccggcgcac ggcgtacgcc
     8701 accgtcggcg ggcaccaaag cgtggcgggc gaaatcctgg aggacgtagc attggccgag
     8761 cgggtcaagg ctgcaggctt gcggctttgg ttcgggcccg gtgcggaagc cgtcgaggtg
     8821 cggatgtacc ggacgttccg cgcgatgtgg gaagggtgga agaagaatct ctaccggctg
     8881 atggggaact ccgcgggggg agcgagacgc gaattgatgg ccacggtgcc gtggattccg
     8941 tgcctcttgc tgctcggcgg attcgtccgg cctgtccttg cactggtggg agtgggcctg
     9001 ctgctgggcc ggcatgcggc gtacgcggcg gcgctgtcgc gtaaccaatt cccggtttcc
     9061 cggatcgtat tctatatgcc cgcggtggcg ctatacgccg ccgttctgct tgcctcgtgg
     9121 aggagccatg ccgaggggcg ggtaagatgg aagggcagag agtacgccgt gggaagtcca
     9181 gcggcggaag gtgaagggcg ctgatggtca cgatcacgcg aaagatcgaa tttgccgctt
     9241 cgcacaggta taacaacccc gcgttgagcg cggaggagaa ccgccgcatc ttcgggaaat
     9301 gcaacaatcc gcacgggcac ggccacaact atatactgga agtgacggtc gccggcgagc
     9361 cggacccgat gacgggaatg gtgctggacc tgaaggagct gaaagagctg ctggaaaaag
     9421 aagtgatgca gcggatggac caccggcatt tgaatcatga ggtgccggag ctcgcgggga
     9481 aaattccgac ttgcgaaaac atcgcgcagg tgatctggga gctgctggag ccgaaaatca
     9541 cgcgcgggcg gctgcaccgg atccggctgc acgagtcggc ggacctgttc gcggattgct
     9601 accgcaatgg agcctcgggg gcatgagcgg agcgggcaag ggcgatttga aaaccgtgct
     9661 ggggcggaga taccgttttg cagcatcgca ccggcttcac a
//
LOCUS       DASYVO010000128        14288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_115198, whole genome shotgun sequence.
ACCESSION   DASYVO010000128 DASYVO010000000
VERSION     DASYVO010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 14288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14288
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115198"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1558)
                     /gene="kaiC"
                     /locus_tag="VGR03_04185"
     CDS             complement(101..1558)
                     /gene="kaiC"
                     /locus_tag="VGR03_04185"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HEV1993510.1"
                     /translation="MATPTRRAQGRDAAGALQKRKALPKVATGIAGLDKVLGGGLPAG
                     RMTLLSGGAGSGKSMMGLQCLLQGAGTGEPGILVMFEERAAAVRQNAWSLGWDLAPLE
                     KKNRIYLMDARLDPEAVISGDFSIRGLLAILDQRIKAMRARRIMIDAVDALLHLYDSP
                     LRERHEINTLHEWLLDRGLTTIMTVKTIPQEEAPSRYAFLDFMADCVIHVDQRVTAQV
                     TTRRLRVIKYRGSGYGRNEYPFIISGDGITVIPITSVAIQHRPLGPKVSSGQSQLDDV
                     LAGGYKRGASVLIVGTAGAGKTTLACMFALATCRRGERVLYLNFEESPESMVGSMLSP
                     GLGLRPLIKSGTLVVRSYLPEAMGVEEHLFHALKALDEFQPQHVVVDSISACQRMGSE
                     GAAFEYLMRLMNACKERGITCLYISQATGLDIVTEISGIGISSITDTIVLLRHVPIGG
                     VMKRELIVMKSRGSKHSEQFHEFRITDRGIDLGKA"
     gene            2001..2648
                     /locus_tag="VGR03_04190"
     CDS             2001..2648
                     /locus_tag="VGR03_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1993511.1"
                     /translation="MVTIVLADDHAVVREGLRLLLESDPEFAVVGEAADGLEAIELVK
                     RRKPKVLIVDLMMPGLDGLETTRRVLRLKLKTRVVILTMYGDEAYLLDALGSGAAGFV
                     VKESCGADLFQAIRDVVAGRRYLSPLLSEDSTRRYLKKFKTALLKLSDTLTTRERKVL
                     QLVLEGATSCDIGARLKISLRTVESALANFVKKLGPDTGPDLIRHGVKRGVTAGG"
     gene            2910..3593
                     /locus_tag="VGR03_04195"
     CDS             2910..3593
                     /locus_tag="VGR03_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV1993512.1"
                     /translation="MATLIAVKSNKKRAFNAQAFLDSAGVARKVAEFPRAATVFTQGE
                     PAKKVFYIQKGGVKLSVVNAVGKQVVVAILGPGDFLGEGCLAGQPIRIGTATAITPTT
                     LLAIDREEMVRVLHEEHALSDRFISHMLSRNIRIEEDLVDQLFNSSEKRLARTLLLLA
                     RYGKQGQPHTALPKVSQETLAEMIGTTRSRVNFFMNKFRKLGFIHYNGGVNGGLQVHN
                     SLLSVVLHD"
     gene            complement(3865..4569)
                     /locus_tag="VGR03_04200"
     CDS             complement(3865..4569)
                     /locus_tag="VGR03_04200"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEV1993513.1"
                     /translation="MLRRFTITLCILFAAATVYAANKEQKRLETCGTVIQEIMNLPDN
                     IPQELLEKADCVIVIPSLKKLALGIGGSYGRGAMVCRTGEKFNGAWGAPAMYALEGGS
                     VGFQIGGQSTDLVLLVMNEKGANSILSSKVKLGGEASAAAGPVGRNASANTDLLMQAE
                     ILSYSRVQGLFAGISLEGSTLRPDDDASADVYGRKLTAREIVLGGKTETPASGRHLIR
                     VLQKYASHKEAKDASR"
     gene            complement(4718..4942)
                     /locus_tag="VGR03_04205"
     CDS             complement(4718..4942)
                     /locus_tag="VGR03_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993514.1"
                     /translation="MKPIMIAGIVLVVLGALALAYHGITYTTQEDVINVGPIHATTDR
                     QERIPLPPVLGGLALAGGIALLVIGAKKQT"
     gene            complement(4960..5112)
                     /locus_tag="VGR03_04210"
     CDS             complement(4960..5112)
                     /locus_tag="VGR03_04210"
                     /inference="COORDINATES: protein motif:HMM:NF033488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEV1993515.1"
                     /translation="MLWTIFVVLLVLWLLGVVSSYTMGGFIHILLVVAIAVVLIRVIQ
                     GRKIIV"
     gene            5925..6581
                     /locus_tag="VGR03_04215"
     CDS             5925..6581
                     /locus_tag="VGR03_04215"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEV1993516.1"
                     /translation="MKLVSVNTGLPREVTWRGRNVTTAIYKQPVEGRVALRQLNLDSD
                     GQADLSVHGGEQKAVYCYPVEHYDYWKRELPGHDLPMGSFGENFTTDGMLEDSVHLGD
                     QFSVGSAEVIVTQPRLPCYKLGVRFQSDDMVKRFLASGRTGFYLAVTREGEVGAGDEI
                     KVIARDPNAVSVSEITRLYVTKRFSDADVAPLRRALRVAALPEGWKAYFRERLGKMNV
                     "
     gene            complement(6899..7144)
                     /locus_tag="VGR03_04220"
     CDS             complement(6899..7144)
                     /locus_tag="VGR03_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993517.1"
                     /translation="MTQAKTERAKSYFRAVEYGDGTPWIICEPQGSNKLEALRDGFIG
                     FDLRPGTSLKEASRIAELLRETVTDVSVTVFERERSY"
     gene            complement(7484..8353)
                     /locus_tag="VGR03_04225"
     CDS             complement(7484..8353)
                     /locus_tag="VGR03_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HEV1993518.1"
                     /translation="MNESNAKPPKFLDPYFARFASYVDETGHSKDPKRMYFCLAGLVA
                     TQEVWKSFDTEWRQVAAEESINLPFHMKDFAHSHRQFAGWSEERRQRLLGKLMTTIRR
                     AAAIPVGSVVSVKAFNALEPKFRSGLRDPYFVAFQHLTWHMVVAASLNIPGGPVSMVY
                     AHHPEHSRGRGNSEQLWDVYRRGNPILAPFMENYVGGQPKDHTPLQAADIWAYELGHH
                     FERIRPSGKNPRWAYRQFVEMGVKAIYGHDFLTVNDAAGVHGVGAYSQATRGTQWNGR
                     VVESLEPDSSPGS"
     gene            complement(8505..8985)
                     /gene="rnpB"
                     /locus_tag="VGR03_04230"
     ncRNA           complement(8505..8985)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGR03_04230"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(9077..10252)
                     /locus_tag="VGR03_04235"
     CDS             complement(9077..10252)
                     /locus_tag="VGR03_04235"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEV1993519.1"
                     /translation="MSDTAEKHSIIDSAATQCILIAEDQPDVSEALRLLLKGAGFKTE
                     WANSPARVLQIVEQRSFDAVLLDLNYARDTTSGEEGLDLITRLRAIDPILPLVVMTAW
                     STVDLAVQAMRLGANDFIQKPWENARLLALLRKQISLAADGRRESDRLRLERHDAEEA
                     REIQRSLLPTELPQIPGVEISADWLPARGVSGDYFDVLKFSDTRVGFCIADVAGKGMA
                     AALLMSNLQATVRAFATPDRAPRELCEKVNGILCKNQVPGRFITFFYGVLDARGKSLA
                     YCNAGHNAPILHRRDGSFMRLEDGGPVLAEFPGQAYAQGAAPVAAGDRLLLFTDGVSE
                     ARSADGDEFGEERLLKVLAAHRGLGALELRRKLLQDVHEFSERPLHDDATLLVLASL"
     gene            10788..13715
                     /locus_tag="VGR03_04240"
     CDS             10788..13715
                     /locus_tag="VGR03_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993520.1"
                     /translation="MRRTLVCLTLLFGFAAGGFAQQKPSPDKRPPGPARKGAYAVDAG
                     GRSIRALENGSTLFVGARGLRPNTTYEFRLGIGRESLPSLEGAVSFARVTTDRRGGVP
                     PFVLWYESGVAGCSDQMSERRKAPPNTYRTFEEAEEALHGKTLLVSAHAVEKSESGRV
                     PPMKLRVDREPESSFRLPILPRRSPEVYASDKDGCMLNSQLAQRGDLYVSGQHFRPGE
                     SLQISVVPNQRLWYVGDAINDVTGVDFAAASERVTVGPDGRFTVKAWDAINQRRGAYD
                     IVAHRPVPAAPASRIGRRDIISYAAETGFILYLRYPVGGPTMDIAGRPFSWSPYFEFA
                     DSFADTGDPVWGAVDPTYIPMNHPGGTYAAYYVVNHRDVAGWDPQMGGATNLVDVSGG
                     FEIHAVKSWCVNGTDVIIWPSPLNPGSYDVVVDFGQNPAATPQQYATDGNYDAAVDFL
                     DGADQIGFVVATDPYALGNVPIGQDSYSQDDFFPMLGGAANVDLRAVVRYPATAPGQG
                     MPVAAGQHPIFVIEHGNHFLCAVGKDGRTTAQEYADARAGIVTWAQFSAWQYDHVTCP
                     NRTKNHEGYMHLLDALASHGIIAVSIDAYDLTGPVPGWIVERGTLILKHLELWSHMNN
                     PQTFMTYPDFFNGRFANHVDLTKISVSGHSRGGEASVAAYMLNNFFTIGSVSSIAPVD
                     FTGYVLPDVPYFVIIPAADGDVFSLNGVPIYDRAGSGLQPPDATLKSGIDVYGASHNF
                     FNTVWAADFDDNTDPNRPDYIPAAEQQKIGEAYLAAFARIHLNNEVVYMDMMRGKLTF
                     PSTAGRKIYPFHHEKLHSKLENGTNMGALAAGGAAVMSVNNPSVHQTAALRVGWPTGT
                     ATLTYTIPMNMRDVSGFEVLSFRVAQTNSMTNPVMGDQDFEVELQGGGKTKTTFAFNY
                     GHIPRPYDSRGQNVMTTIRIPLHSFIMNNSGVTLNNVDTLVFRFLLPTQGEIYVDDIE
                     FSR"
     gene            13687..14229
                     /locus_tag="VGR03_04245"
     CDS             13687..14229
                     /locus_tag="VGR03_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993521.1"
                     /translation="MWMTSNFRGRACWLSFLQAALVLWTPPPASAAKAQQTTAPQIAP
                     DSPRPSVARRRPAGGGKELSVKAMTVELKSASVRHLDEKMKAMAGAEFLKAAREPLSI
                     EVKTVNPLGNLSRSSLPVIVLNGEKLPGTRATNQTTLVAFLPDRSMLKDSNTIAVVWL
                     GDERTRTKYPLTLKLEEIFR"
BASE COUNT         2806 a         4359 c         4072 g         3051 t
ORIGIN      
        1 gtcgcgtctc tgcccctctt gcgcttgcga gctttgctca tgctgttcca cttcttccgt
       61 gtccgctcgg atacgccgat ctgcacatcg taaagctggg ctaggccttc cccagatcaa
      121 tccctcgatc ggtgatgcgg aattcatgga actgttcaga atgcttcgag cctctcgact
      181 tcatgacgat caactcgcgc ttcataaccc caccgatcgg aacatggcgg agaaggacga
      241 tggtatcggt aatggaggaa attccgatgc cgctgatctc tgtgacgata tcaaggcccg
      301 tagcctggct gatataaaga catgtgatac cgcgctcttt acaggcgttc ataagccgca
      361 tcaggtattc aaaggcagca ccctcagagc ccatgcgctg gcacgccgag atcgaatcca
      421 cgaccacgtg ctggggttga aactcatcca gggctttcaa ggcatgaaac aagtgttcct
      481 ccacgcccat cgcttccggc aagtaggacc ggaccaccaa cgtcccggat ttgatcagcg
      541 gccgtaggcc caggcccggg ctcagcatgc tgccaaccat cgattctggc gactcctcga
      601 aattgagata aagcacacgc tcgccgcgcc ggcaagtggc cagggcaaac atgcaggcca
      661 gggtggtctt tcccgcgccg gcggtcccca cgatcaggac gctggcccca cgtttgtagc
      721 caccggcgag cacgtcatcc aactgggact ggccgctgga cactttgggt ccgagcgggc
      781 ggtgctgtat ggcgacgctg gtgatcggaa ttacagtgat gccgtcccca ctgatgatga
      841 agggatattc attgcggcca tatcccgaac cacgatattt gatcactctc aggcggcgcg
      901 tggtgacctg agcagtgacg cgctggtcca cgtgaatcac acaatccgcc atgaagtcca
      961 gaaatgcgta ccgggaagga gcctcctcct gcgggatcgt tttgaccgtc atgatcgtgg
     1021 tcaatccccg gtccagcagc cattcgtgca gggtatttat ttcgtggcgc tcgcgcaggg
     1081 ggctgtcgta caaatgcaag agcgcgtcca ccgcatcaat catgatgcgc cgtgctcgca
     1141 tggctttgat ccgctgatca aggatggcca gcagacctct gatggagaag tcgccggaga
     1201 tcaccgcttc aggatccaaa cgcgcatcca tcaggtagat cctgtttttc ttctccagcg
     1261 gtgccaggtc ccatcccaaa ctccaggcgt tttgacgcac ggcagcggcg cgttcctcga
     1321 acataaccag aatgcccggc tcgccggtac cggcgccctg taacaggcat tgcaagccca
     1381 tcatgctttt gccgctcccg gcgccgccgc tgagaagcgt catgcgtccg gcgggcaggc
     1441 caccgcctaa cactttatcc agcccggcaa tgccagtcgc aaccttgggg agcgccttcc
     1501 ttttctggag cgcgcctgct gcgtctctgc cttgcgccct tcgtgttggt gtggccaccg
     1561 caggccctcc ttgattccgg gtttctcccc agactgtcag tccaagcatc agcggaacgc
     1621 cactggtatg cctctcttcc gcaatcctat cccaggcaaa aagaaaagca tacttcggat
     1681 tagggcttga ccatgctcgg gtgtggttgg cgcgatctcc gttcggcaga aaactttgca
     1741 gtcaaacgag agacaggctt tcttactccg gctccgttgc ggcgcggtca aggatttggt
     1801 ccccagccca ggaaaccagg aatggcagtc catctctgcg ggaaagtttc ggtttttctg
     1861 atggggctcc atgatactct aaattcttgg ggccatattt tgatgaagcc ggctccaaac
     1921 gcagaacccg gtgtgacagc ccacggttcg ttgttgcgcg tctcggaatg aaagagggct
     1981 caaggttttt ggagggcgta atggtgacca tcgtgctggc tgatgaccat gctgtcgtga
     2041 gggaagggct gcgccttcta ctggagtcgg atccagaatt tgccgttgtg ggtgaagcgg
     2101 ctgatgggct cgaggcgatc gaactcgtca agcgccggaa gcccaaggtg ttaatcgtcg
     2161 atctgatgat gcctggcctg gacggtttgg aaaccacccg ccgggtcttg cgcctgaaat
     2221 taaagactcg tgtggttatt ctgacgatgt acggagacga ggcgtattta ttggatgcac
     2281 tcggaagcgg tgcggcgggt tttgtcgtca aagagtcctg tggagctgac ctcttccaag
     2341 ccatacgcga tgtggttgcc ggtcggcgtt acctgagtcc actgctctcc gaggattcca
     2401 ctcgcagata tttgaaaaag tttaagactg ctttgctgaa actctctgac acactgacaa
     2461 cccgcgagcg aaaggtgttg caactcgttt tggagggggc caccagttgc gatattggtg
     2521 cccggctgaa gatcagtctt cgcactgtcg aatccgccct ggccaacttt gtgaagaagt
     2581 tgggcccgga caccggccct gaccttatcc gccatggagt caagcgcggt gtcacagctg
     2641 gagggtaacc caggcagatc aggaaaaaaa taatcaattc agggatatcg aactgaaacg
     2701 agcatgtcct aggtctcgtt gccggtcacg cgcttcccgg gaagaagtcg attgaggtta
     2761 ggtcctgctg cggaaagctg aaccccgcgc tcttcgctgt tggatttcct taccaaaacc
     2821 ctcaagcatc catccgtttc caagggggtc ttgactcgat ttgcatggtc ctattagaat
     2881 gcgctctgcg ctgggaggcg accgaggcca tggcgacttt aatcgctgtg aaatccaata
     2941 agaagcgtgc tttcaatgcc caagctttcc tcgattcggc cggcgtggcg agaaaagttg
     3001 cggagtttcc aagagctgcg accgtcttca ctcaaggtga gccagccaag aaagtttttt
     3061 acattcaaaa aggcggcgta aagctttccg tggtcaatgc ggtcggcaaa caggtcgtcg
     3121 tggcgattct gggaccaggc gactttcttg gcgaggggtg cctggctggt cagccaattc
     3181 gtattgggac ggctaccgcg attacaccga ccactttgct ggctattgat agagaggaga
     3241 tggttcgcgt gctccacgag gaacatgcgc tctccgaccg tttcatctct cacatgctgt
     3301 cacgtaacat ccgcatcgaa gaggacctgg tcgatcagct ttttaactcc agcgagaaac
     3361 ggctggcccg cactcttttg ctcctcgcgc gctatggcaa gcaaggccag ccccacacgg
     3421 cgcttcccaa agtatcgcag gagacgctgg cggaaatgat cggcacaacg cgctcgcggg
     3481 tgaatttctt catgaataaa ttcaggaaac tgggcttcat tcactacaat ggaggagtca
     3541 atggcggact ccaggtccat aattcccttc tgagtgttgt cctgcacgat tgacgaccaa
     3601 tccttcccgc ggcgcaatat cggcttggcg gatgtctccc tttttccttt ttgaaattct
     3661 gcaggtctcg cccacgcgcc ggcccccctt tttgacctgc aattgaaaat cagtctctgg
     3721 gctatggatc gtgaaaacgg gaggaggcgg gccatccgcc agaagcgttt gggcgccttg
     3781 gcggatggcc ccttgggggg tgtccggggt aggacttcgc tgagccgctc ttctattcga
     3841 cgaagagcgg gaaatcatcc gcgactatcg agacgcgtcc ttcgcttcct tgtgcgatgc
     3901 atacttctgc aaaacacgga tcagatggcg gccggacgct ggagtctcgg tttttccgcc
     3961 cagcacgatt tctctcgccg taagtttgcg gccatagacg tctgcgctgg catcgtcatc
     4021 cggccgcagc gttgatcctt ccagggagat accggcaaag agaccctgca cacgggagta
     4081 gctgaggatc tccgcttgca tgagaaggtc ggtgttcgcg gaggcattgc ggcctaccgg
     4141 gccggccgca gcagaagcct caccacccag cttgaccttg ctgctcagaa tagaattggc
     4201 gcctttctca ttcatgacca gaagcaccag gtcggtggat tgtccgccga tctgaaatcc
     4261 aacgcttcca ccttccagcg catacatggc gggagcgccc caggctccat tgaatttctc
     4321 gcccgtgcgg cagaccatcg ccccgcggcc atagcttccg ccgattccaa gcgccaactt
     4381 cttgagagat ggaatgacga tcacgcagtc ggctttctcc agcagttctt gaggaatatt
     4441 gtcgggtagg ttcatgattt cctgtatgac cgtgccgcaa gtttccaggc gcttttgttc
     4501 tttgtttgcg gcgtagactg tggctgctgc gaataaaatg caaagcgtaa tggtgaaacg
     4561 acgtaacatt tgtgacctcc gtaatcagac ttgtcgttgc taattccggg ctaactctgg
     4621 ctctctcttc aatccctgaa aggatgttct gaactcgggc tgctcgtggg gctagcccgc
     4681 ttgttgcacg ggccttacgg atgctccccc aattcagcta ggtttgtttc ttcgcgccaa
     4741 tcaccagcag agcaatccct ccggccagcg ccagtcctcc cagtacggga ggaagtggaa
     4801 ttcgctcttg ccggtctgtc gtggcgtgaa tcggcccgac attgatcacg tcttcctgcg
     4861 tcgtgtaagt gattccgtga taagcgaggg ccaacgctcc aagaaccacg agcacaatcc
     4921 cggcaatcat gatcggtttc attgtcgtcg ctctcctggt taaacgatta tttttcggcc
     4981 ctgaatgacc ctgatcagca ctacagcgat ggccacaacc aacaggatgt gaatgaaacc
     5041 acccatggtg taggaactca ccacccccaa gagccacaag accagcagca ccacgaatat
     5101 tgtccagagc atgttggtcc tccttacctg caggttcgaa atacgttctc gtgcgccccg
     5161 tcatcccggt aagcgcatcc cttgagtcga tcgcagaccc cgcaagggac acgaaacgaa
     5221 ttgatgccgg gagtctcccc cgaagcgttc ctgtttctgt tttcaggccg gaaggcggag
     5281 cgggagccga atggctggtt ttcacaccag tcgttccaca tcttccccag acaccatcgt
     5341 agaggaagca aggaggagtg tctgttcaat aaggaacacc ttctcatttc gagtgaagat
     5401 cagcgaccat gcccgatgac gcgattaaga tgaacccggt gctccagcct tgcccgcaaa
     5461 ggacctccag gaatggtctg cgttcgcctc gcaattcccg ccgtgactga gcgaggaacc
     5521 cctcttcacc tcagctcagt tcctcgcacg gcatcgcaga cgctgcgcga aggctcgccg
     5581 gggctagccc ctttccggca ctctcctgaa ggtgtggttc aaaccgccct gcttgttcat
     5641 gcgaaatact tgccttcggc ggcgaacttc gccgtgggga ttgaggagag ccgggctgtt
     5701 agtcacgccc acttgagggt atggctcttg tccgacaagg gccgagcgcc acccccgctt
     5761 tgcaacgatg ggctgcccgc ctgtatgctg tgaacggtcg tttctttccg tcgagcggcg
     5821 ccgtaaaccc cgcccttacg attatggccg atgcggtgcg tgtcggcgat caactgcttg
     5881 agtggcttca ctgatttgag gcggatggaa aggagcagcg agggatgaag ctagtctcgg
     5941 tgaacaccgg actcccccgc gaagtgacct ggcgaggtag aaacgtaact acggccatct
     6001 acaagcaacc ggtcgaaggc cgcgtcgctt tgcgccagct taatttggac agcgacggtc
     6061 aggccgacct cagcgtccac gggggcgagc agaaggcagt ttactgctac ccggtcgaac
     6121 actacgatta ctggaagaga gagttgcctg gccacgattt gccgatgggc agctttgggg
     6181 aaaacttcac caccgatggc atgctggaag actccgtcca tcttggcgat cagttctccg
     6241 tcggttctgc ggaagtgatc gtcacccagc ctcgcctccc ttgctacaag ctcggtgtga
     6301 gattccagtc cgacgacatg gtgaagcgat tcctcgccag cggccggacg ggtttctacc
     6361 tcgccgtcac tcgcgaggga gaagtcggcg ccggcgatga gatcaaggtg attgcgcggg
     6421 accccaacgc cgtctccgtc tcggaaatca ctcggctata cgtcacaaaa agattcagtg
     6481 atgccgacgt ggctccgctg cgccgtgcct tgcgagttgc cgcattgcct gaaggttgga
     6541 aggcgtactt ccgggagcgg ttggggaaga tgaatgtctg attgcgggaa ggggctcgat
     6601 tctcggtctg cgcgctgcga gcttcagcct agggagtgag tacctccgga ctgcaagtca
     6661 cgcgaaatag agggtatggc tcttgcctga caccggctgc gccccacccc tgcttcgcaa
     6721 ggatgggcca tccgccaatt aacggcggga gcgtttgcgg gacgtcgagc gcttacggcg
     6781 ctggcccgca cgcaggacga aacctgaaag gctgcggtgt tttcgattct tggaatgcat
     6841 ttcctgaccg gcatcgcgct caatgaaccg agacaggctt gggcattcag tggccatgtc
     6901 agtaactcct ttcgcgttcg aaaaccgtga cagaaacgtc cgtaaccgtt tcccttagca
     6961 actccgcaat cctgctagct tctttaagag acgttcccgg ccttagatca aaaccgatga
     7021 atccgtcgcg cagggcttct agcttgttgc tgccctgtgg ctcacagatt atccacggcg
     7081 tgccgtcccc atactccacg gccctgaaat acgatttggc tctctccgtc tttgcctgtg
     7141 tcatcaaatt ctccaatttg ttcgggctag ctttcaaaat gaaactaccc cttagatagt
     7201 atgagtgtct cacaacgggt ggtagcttta caatggaagt tgtctatttg tagcggtgtg
     7261 tgagccagtc acgacttgat aggctggccc atcccacccg aaccttcggg gagggagggg
     7321 tttcgttcca agcggccaag cgattacgga attcagtggg gcaagactgg cgcactattc
     7381 ttcgatgcag gcggagaaca aatgaaagtg gcggacgagt ctgtaagcag gattctgtcc
     7441 tgcgcgtgtc caacgttcag ccttgcggtg ttcaatggcc caatcaactt ccgggcgacg
     7501 aatctggctc cagagactcg actacgcgac cattccattg agttccgcga gttgcctggg
     7561 aatatgcacc cacaccatgt acccccgctg cgtcattgac tgtaagaaaa tcatgcccgt
     7621 agatagcctt cacacccatt tcgacgaact ggcggtaagc ccaacgtggg ttcttgccag
     7681 acggacgtat gcgttcaaaa tgatgcccga gttcgtatgc ccagatgtca gctgcctgaa
     7741 ggggagtatg gtcctttggc tgtccgccca cgtaattctc catgaaaggt gcgagaatgg
     7801 gatttccgcg gcgataaacg tcccataatt gctctgagtt cccgcgtcct cgcgaatgtt
     7861 cagggtgatg cgcgtatacc atcgaaaccg gtcccccggg tatgttcaag ctcgctgcca
     7921 ccaccatgtg ccacgtcaag tgttgaaaag ccacgaagta tgggtcccta agtccgcttc
     7981 tgaacttcgg ctcaagcgca ttgaatgctt taaccgagac cacgctgcca actggaatag
     8041 cggctgcgcg tcggattgtg gtcatcagct ttcccagtaa ccgttgccgc ctttcttcac
     8101 tccatcccgc aaactgacga tgcgagtgcg caaagtcctt catgtgaaac ggcaaattga
     8161 tactttcttc ggcagctacc tgtcgccact ctgtgtcaaa ggatttccac acctcctgcg
     8221 tcgcaactaa tcccgcaaga cagaagtaca tccgtttcgg gtccttggag tgtcctgtct
     8281 catcgacata agaggcaaat cgcgcaaagt atgggtccaa aaactttggt ggtttcgcat
     8341 tcgactcgtt catctctgct tccattgccg caccaagacc atcaactacg accagtcaaa
     8401 ttcccgacga aagatttacg accgccaacg tagatccgaa ctcgactgtg agcgaatgag
     8461 cctcgtcgcc gttttccttt agcccctgca agggaccgta atcattggcg gacaggtctg
     8521 taagccggat tctgtcctgc gcgtgtccag tttcggacaa cgcgccggga cgatcattcc
     8581 tctgggccac gcatcgctgc gcggctcatg cagcctaccc gagggtcatc tcccggcctc
     8641 ttgcgaaacc gggggtacgg gccggaccag ccctcccctc ctatttggcc ttgctccgcg
     8701 cggggtttac cgtgcctcga gcgtcgccgc tcgagcggtg ggctcttacc ccaccttttc
     8761 acccttaccg gacgcgtccc accgatcgcg agcgttccca ggtttggcct gagttggccc
     8821 acagctcggg acgcgccggc ggtattttct ctgtggcact ttccgtagcc gccccctcgc
     8881 gagtgcggcc ccctggcgtt accaggcgcg ctgtcctgcg gagtccggac tttcctcccg
     8941 cccctccgat cactcggagc gaccagcgat cgtccgaccc gcccgccatc ttcaagtata
     9001 gcgcgataca ggggatgtag cgctgggctt cagcccagca gcttgaatcg ggaatccgct
     9061 gccctgaagg gcagcgctac aaagaggcga ggactaggag cgtggcgtcg tcgtgcagcg
     9121 gccgctcgct gaattcgtgc acatcctgca gcagcttgcg tcgcaattcg agagcgccga
     9181 ggccgcgatg cgcggccagg actttcagca ggcgttcttc gccgaactca tcgccgtcgg
     9241 cgctgcgcgc ttcggacact ccgtcggtga acagcagcag cctgtcgccc gccgccacgg
     9301 gtgcggcgcc ctgtgcgtag gcctgcccgg ggaactcggc gagcaccggt ccgccatctt
     9361 cgagacgcat aaaagatcca tcccgccgat gcaggattgg cgcgttgtga ccggcgttgc
     9421 aataggccag tgattttccc cgcgcatcca gcacgccata gaaaaacgtg atgaatcttc
     9481 ccggcacctg gttcttgcaa aggatgccgt tcaccttttc gcacagctcg cgcggcgcgc
     9541 ggtcgggcgt ggcgaacgcg cgcacggtag cttgcaggtt ggacatcagc agcgccgcgg
     9601 ccatgccttt gccggccacg tcggcgatgc agaagccgac gcgcgtgtcg ctgaatttga
     9661 gcacgtcgaa atagtcgccg ctcacgccgc gcgccgggag ccagtccgcc gatatctcca
     9721 cgccgggaat ctgcggaagc tcggtcggca agaggctgcg ctggatctcc cgcgcctctt
     9781 cggcgtcgtg gcgctccagg cgcaggcgat cgctctccct gcggccgtcg gcagcgaggc
     9841 tgatctgctt gcgcagcagc gcgagcaatc gcgcattctc ccagggcttc tggatgaagt
     9901 cattggcgcc gagccgcatg gcctgaacgg ctaggtccac ggtactccat gcagtcatca
     9961 ccaccagcgg caatatcggg tcaatcgcgc gcaggcgggt gatcagatcg agtccctcct
    10021 cgccagaggt ggtgtcgcgc gcgtagttca gatccagcag gacggcgtcg aagctgcgct
    10081 gctcgacaat ttgcagcacg cgcgcgggcg agttggccca ctcggtcttg aaaccggcgc
    10141 ccttcagcag caggcgcagc gcctcgctca cgtcgggctg atcttcggcg atcaggatgc
    10201 actgcgttgc cgccgagtca atgatggagt gcttttcggc cgtgtcgctc atcgttcgca
    10261 tcgtctcacg tgtcggggat aggagtggca tttgaggggc cacccaaaag catctgtaag
    10321 tctttcgact gaaacggttt gccgccgccg tggccctcag ggtcgatgcc gctccgcccg
    10381 gttgtgggac atactgtcgc ggaagcggac accgcgcgca tccccgtctc ctccccgcct
    10441 gggccaggcg aatcacattc gtggccgctc gatagcttaa acggaacgcc ctgcggtgcc
    10501 cgcgctctgc cggccatcgc gcagctcgcc agccgtggac cggttgcccg cattccccct
    10561 cgaccgtcgg ttattgcctc ttgacatata cgacaggtgg taatataagc gagccattat
    10621 aattgtgaag ccagagggag gtccttgatt cgttttcctg gctcccgcgc aactctttct
    10681 ctgatcgact ttttcatcct ggcccggctc acgaaattgt cgaggaaacg cgcgttggga
    10741 atgttcggcc ccagtcccag cccgatttcc tcgaggaggg ttcctccatg cgccgcacac
    10801 tggtctgttt gactctgctc ttcggattcg ccgccggcgg attcgcacaa caaaaaccct
    10861 ctccggacaa gcgtccgccg ggtcccgcgc gaaagggcgc gtacgcggtg gacgcgggcg
    10921 gccggtccat ccgcgccctg gaaaacggct cgaccctatt tgtcggcgcc cgcggcctgc
    10981 gcccgaatac gacctatgaa ttccggctgg ggatcggccg tgagtcgctt ccttcactcg
    11041 aaggagctgt gagttttgcc cgcgtgacga cggaccggcg cggtggagtg cccccctttg
    11101 tgctgtggta cgaatccggc gtcgccggct gctcggatca gatgagcgaa cggcgcaagg
    11161 cgccccccaa tacctatcgc acgtttgagg aagccgagga ggcgctccac ggcaagaccc
    11221 tgctggtctc cgcgcacgcc gtggaaaaat cggagagcgg ccgcgtgccg cccatgaaac
    11281 tccgcgtgga ccgggagccg gagtcctcct tccgcctgcc cattcttccc cgccgcagcc
    11341 ccgaggtgta cgcttcggat aaagatggct gcatgttgaa cagccagctc gcgcagcgcg
    11401 gggatcttta cgtcagcgga caacacttcc ggcccggcga gtcgctgcaa atctccgtgg
    11461 tgccgaatca gcgcctctgg tatgtgggcg acgcgatcaa cgacgtcacc ggcgtcgact
    11521 tcgctgccgc ttccgagagg gtcaccgtgg gtcccgacgg gcgcttcacc gtcaaggcct
    11581 gggacgccat caaccagcgc cgcggcgctt atgacatcgt ggcgcatcgt ccggtcccgg
    11641 ccgcgccggc aagccgcatc gggcggaggg acatcatctc ttacgccgcg gaaacaggat
    11701 tcattctgta tctccgctac ccggtgggtg gtcctaccat ggacatcgcc ggtcggccct
    11761 tctcctggtc gccctatttt gagttcgccg actctttcgc cgacaccggt gacccggtat
    11821 ggggagccgt ggatcctact tacattccca tgaatcatcc cggaggcact tacgcggcct
    11881 actacgtggt gaatcaccgc gacgtggccg gttgggaccc gcaaatgggt ggggccacga
    11941 atctggtaga cgtttccgga ggctttgaga ttcacgctgt gaagtcctgg tgcgtcaacg
    12001 gaaccgacgt gatcatctgg ccgtctccgt tgaatccggg tagctatgac gtcgtggtgg
    12061 acttcggcca gaacccagcg gccacgccgc agcaatacgc caccgatggg aattacgatg
    12121 cggcggtgga tttcctcgac ggcgcggacc aaattgggtt cgtcgtcgcc accgatccct
    12181 acgccctcgg caatgttccc atcggccagg attcctacag ccaggacgac tttttcccga
    12241 tgctcggtgg cgcggcaaat gtggatttgc gcgccgtggt ccgctacccg gcaaccgctc
    12301 cgggccaggg catgcccgtc gctgcgggac aacatcccat cttcgtcatc gagcacggca
    12361 accatttcct gtgcgccgtg ggcaaggatg ggagaaccac cgcacaggaa tatgcggatg
    12421 cccgggcggg cattgtcacc tgggcacagt tcagtgcctg gcagtatgac catgtgacct
    12481 gtcccaatcg cacgaagaat catgaaggct acatgcacct cctcgacgct ctcgccagcc
    12541 acggcattat cgccgtttcc attgacgcct atgatctgac cggcccggtt cccggctgga
    12601 tcgtcgagcg cggcacgctg atcctgaagc acctcgaact gtggtcgcac atgaacaatc
    12661 cccagacgtt catgacttac ccggatttct ttaacggccg cttcgccaac cacgtggact
    12721 tgaccaagat ttccgtctcc gggcattcgc gcggcgggga agcctcggta gcggcctaca
    12781 tgctcaacaa ttttttcacc atcggctccg tctcctcgat tgcgccggta gacttcaccg
    12841 gctatgtgct gccggacgtt ccttattttg tgattatccc cgcggcggac ggggatgttt
    12901 ttagcctcaa cggcgtgccc atctacgacc gcgccggcag cggcctgcag cccccggacg
    12961 caacgctgaa gagcggtatc gacgtctacg gagccagcca caacttcttc aacaccgtgt
    13021 gggccgccga cttcgacgac aacacagacc ccaatcgccc ggattacatc cccgccgcgg
    13081 agcagcagaa gattggcgag gcctacctgg cggccttcgc gcgcatccat ctgaacaatg
    13141 aggtcgttta catggacatg atgcgcggca aacttacctt cccctccacc gcgggccgca
    13201 agatctatcc gttccatcac gagaaactgc actcgaagct ggaaaacggc accaacatgg
    13261 gagcgctagc cgcaggggga gccgcggtga tgtccgtgaa caatccttcc gtgcatcaaa
    13321 ccgcggccct tcgagtgggc tggccgacgg gcaccgccac gctcacctac accattccga
    13381 tgaacatgcg cgacgtctcg ggttttgagg ttctctcgtt ccgcgtggcg caaacaaact
    13441 ccatgaccaa tcccgtcatg ggcgatcagg acttcgaggt agagctgcaa ggtggaggca
    13501 agaccaagac cacctttgcc ttcaactacg gccacattcc cagaccttac gacagccgcg
    13561 gtcagaacgt gatgaccacc atccgcatcc cgttgcactc cttcatcatg aacaactcgg
    13621 gcgtcacgct gaacaatgtg gatacacttg tgtttcgctt cttgttaccg acacagggag
    13681 agatttatgt ggatgacatc gaattttcga ggtagggcct gctggctatc gttccttcag
    13741 gctgctctcg ttctctggac gccgccgccg gcctccgccg cgaaagcgca gcaaaccacg
    13801 gcgccgcaga tcgcaccgga ttcgcctcgc ccctccgtcg cgcgccgccg cccggccgga
    13861 ggaggaaagg aattatccgt gaaggccatg accgtcgagc tgaagtccgc atcggtccgt
    13921 catctggatg aaaaaatgaa agcgatggcg ggagcagagt tcttgaaagc ggcccgcgag
    13981 cctctgtcca tcgaggtcaa gaccgtgaat cctctcggca atctttcgcg ttccagcctg
    14041 cccgtcatcg tgctgaatgg ggagaagctc cccggcaccc gcgcgacaaa tcagaccacg
    14101 ctggtggcgt ttctgcccga ccgcagcatg ctgaaagatt ccaacacgat tgccgtggtc
    14161 tggctgggcg atgagcggac caggacgaaa tatcccctta cactgaagct cgaagaaatc
    14221 ttccgttgag cggcggccgg cgggaatgcg gctcggatca tccgcccgag ccatgtccgg
    14281 cgtgtcca
//
LOCUS       DASYVO010000129         7177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_31430, whole genome shotgun sequence.
ACCESSION   DASYVO010000129 DASYVO010000000
VERSION     DASYVO010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31430"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="VGR03_04250"
     CDS             <1..1191
                     /locus_tag="VGR03_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV1993522.1"
                     /translation="VPKLTGVKWQFHTKVQVLSSPAIAADKLYFGSSDHCLYALDLAT
                     GALKWRFKSEGRITSSPAVSAGVVYFGSYDGNFYAVDAATGQLKWKFQTGGERRFAAK
                     HLHGSEPAAETMPDPFDFYLSSPVIWSGGVYFGSGDTNVYALDAASGKLKWKFKTGDV
                     VHASPAISGGTLFAGSWDSHFYALEAGSGKEVWRFKTGEDPDIHNQVGIQSSATVADG
                     VVYFGCRDSKFYAVDAASGKERWSFSNKGSWVITSPAVLDGKVYYGTSDTGMFHAFDA
                     KSGAPLFSLDFKHWPMFSSPAIAGGTLYIRSNQGRLNAIDLKTQKVAWSFETEDSKKN
                     GPTYTKPDGTPNYEAAFFDFFYDDMVTGVQKMMAVGAILSSPVVAGNIVYVGSTDGNI
                     YALM"
     gene            1492..1881
                     /gene="hisI"
                     /locus_tag="VGR03_04255"
     CDS             1492..1881
                     /gene="hisI"
                     /locus_tag="VGR03_04255"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEV1993523.1"
                     /translation="MEIDFQKSGALVPAIIQDERTGDVLMLGFMNAESLAETQRTGEV
                     VFFSRSRNKLWKKGESSGHVLRVREMRVDCDADALLVRVEAVGPGVCHEGYRSCFFRK
                     LELDGSVAVIAERTFAPEKVYGQEQAR"
     gene            1878..2750
                     /gene="hisG"
                     /locus_tag="VGR03_04260"
     CDS             1878..2750
                     /gene="hisG"
                     /locus_tag="VGR03_04260"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEV1993524.1"
                     /translation="MSLLKLGIPKGSLQEATLDLFSRAGWKITLGSRSYVPAIDDTEI
                     ECLLVRAQEMARYVESGALDAGITGHDWVVETGAAVDELSELIYAKQRLARVRWVLAV
                     PEDCAIREPRHLEGKVIATEVVHITENYLAKHGVKARVEFSWGATEVKVPQLADAIVE
                     VTETGSSLRANRLRIVDTVLESATVFIMNRAASADSWKREKAENLILMLQGAIAAASK
                     VGLLLNVHRDDLQAVLGVLPALKKPTISALSDPDWVAINTIVEEAVVRQILPKLKAAK
                     AQGIVEYPLNKIVL"
     gene            2750..4003
                     /gene="hisD"
                     /locus_tag="VGR03_04265"
     CDS             2750..4003
                     /gene="hisD"
                     /locus_tag="VGR03_04265"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEV1993525.1"
                     /translation="MLIVRLTPAVERRLLRARQEHDGEAQRIASEIIHDIRRRGDAAL
                     SAWVKKLDDIDLQRESLWISEGEIRVARKQVSREFLRALEHAARNVRRVAEKQMPKPW
                     TMEVEPGVKIGQRVRPIDSIGCYIPGGRFALVSTMVMTVVPAKVAGVRHIRVVCPRPN
                     AALLAAAGVLGVTRVARMGGAQAIAALAYGTKRVARVEKIFGPGNRFVTAAKQLVSSD
                     CAIDLPAGPTEAIVLATTGNPRWIAADLLAQAEHAPDAGSYLVTTSTRLARQVQTEVA
                     RQLHELPSTNPAQISMKRAGAILVAPSVHAACEFVDRFAPEHLSLPGNGESLLKQIHA
                     TGTIFLGPWGAQPLGDYASGSNHVLPTGGWARKRGGLSAADFVKCISVQSIGRSGFAR
                     LAADVQTLARAEGLLAHANAVEVRR"
     gene            4000..5064
                     /gene="hisC"
                     /locus_tag="VGR03_04270"
     CDS             4000..5064
                     /gene="hisC"
                     /locus_tag="VGR03_04270"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEV1993526.1"
                     /translation="MKVHLPVRKAILNRRTYEAPAEGRSDKLRLDFNENTAGCSPAVR
                     RALAKLTTKQLAMYPEYQAPTRRIARYFGVRPEELLLTNGGDDALRVFFDTFVESDSS
                     ILICEPTFPMYRYYAEIFGARISVLHYGSEMEFPLKGAVAALRKKPRVLFIANPNNPT
                     GTLLQKEELRRILKAATHTAVVMDEAYAEFSGFTAVPWVRKYPQLFVAKTFSKVAGLA
                     ALRLGAVIACEESLALVRRAMPPFPVNLAALVATEAAVADRATMQGYVRGVKRLRAWF
                     AEELQKLGVKTYRSAGNFLLANFGTSGLQLFQKLEKQGILLREKSKDIGPGFVRITIG
                     TQSEMRRLLKTFRKEWKAPA"
     gene            5091..5681
                     /gene="hisB"
                     /locus_tag="VGR03_04275"
     CDS             5091..5681
                     /gene="hisB"
                     /locus_tag="VGR03_04275"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEV1993527.1"
                     /translation="MPRTATIHRKTNETDIRLKLHLDGRGKARIATGIRFFDHMLELV
                     ARHGAFDLDVDACGDLDVDQHHTVEDVGITLGEAVQKALGSKRGILRAGYFLMPMDET
                     LAAAAVDLGGRPYCVVNAKVAAKRVGDFQVELMEDFFQGFSQAAKANVHLRALYGRSS
                     HHQVEAMFKAFARALRFAVSRDKRLRKVLPSTKGLL"
     gene            5678..6283
                     /gene="hisH"
                     /locus_tag="VGR03_04280"
     CDS             5678..6283
                     /gene="hisH"
                     /locus_tag="VGR03_04280"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEV1993528.1"
                     /translation="MKVTIVDYGAGNVPSVERALQRLGAESERTSSPECISKAEALLL
                     PGVGHYAALVRALDEKTLRAPLVEAIHRGVPFLGICLGLQVLFKSSEEASQLQGLNLL
                     PGIVSALPPGVKLPHMGWNQLMANRDSCLLAGIDAGAYFYFAHSYAALDSNGAAAATC
                     SYGAEFAAVIEQENIYAVQFHPEKSGDPGARVLQNFLRRAA"
     gene            6280..7044
                     /gene="hisF"
                     /locus_tag="VGR03_04285"
     CDS             6280..7044
                     /gene="hisF"
                     /locus_tag="VGR03_04285"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEV1993529.1"
                     /translation="MSLAHRIIPCLDTDGERVVKGVNFAGLRDAGDPVALAARYNAEG
                     ADELVVLDIAASRDRRPTFLDTIRRVAAELAIPLTAGGGIRTLEDGRAVVRAGADKVT
                     VNTAAVERPELISELSREFGAQAVVLAIDAKRSGDHWDVMVRGGRESTALDAIEWARM
                     GVAAGAGEILLTSVDRDGTQSGFDVALTAAISRAVTVPVIASGGAKSPEHFVEIFQEG
                     AADAALAASIFHDEVQSIRSLKEFLAARGVEVRLPC"
BASE COUNT         1426 a         2071 c         2213 g         1467 t
ORIGIN      
        1 gtcccgaaac tcacgggtgt caagtggcag tttcacacca aagtgcaagt gctttcgtcc
       61 ccggcgattg cggccgacaa actctatttt ggcagcagcg atcattgtct ctacgctctc
      121 gatctcgcca ccggggcgct caagtggaga ttcaagtccg aaggccgcat cacctcatcg
      181 cccgcggttt ccgcgggggt cgtttatttc ggcagctatg atggcaattt ctacgctgtg
      241 gatgccgcga ccggccaatt gaaatggaag tttcagaccg ggggtgagcg ccgctttgcc
      301 gcaaagcatc tccacggctc cgagcccgct gcagagacca tgccggaccc gtttgatttt
      361 tacctgtcct cgcccgtcat ctggagcggc ggagtctatt tcggcagcgg agataccaat
      421 gtgtatgcac tagatgccgc ttcgggcaag ctgaaatgga aattcaagac tggcgacgtc
      481 gttcacgcct ccccggctat ttcagggggc actctgtttg ccggaagttg ggacagtcat
      541 ttctacgcgt tggaggcagg cagcgggaaa gaggtttggc gtttcaagac gggcgaagat
      601 ccggacatcc ataatcaggt cggcattcag tcctccgcga ccgtggcgga cggtgtagtg
      661 tacttcggct gccgcgattc gaagttttac gcggtggatg cggcttccgg caaggaacgc
      721 tggtccttct cgaacaaggg ctcctgggta atcacctccc cggccgtgtt ggacggcaag
      781 gtctactacg ggacttcgga cacgggcatg ttccatgcct tcgatgccaa gtccggcgct
      841 ccgctcttct ccctcgactt caaacattgg cctatgtttt cttcgccagc gattgcaggc
      901 gggactttgt acatccggtc taatcaggga aggctcaacg ccatcgacct caaaacgcag
      961 aaagttgcct ggagctttga aactgaggat tccaagaaaa acggcccgac ctacaccaaa
     1021 cccgacggca ccccgaacta cgaagcggct ttcttcgact ttttctacga cgacatggtt
     1081 accggcgtcc agaagatgat ggcggtcggg gccattcttt cctctcctgt ggtggccggg
     1141 aacatcgtgt atgtaggcag caccgacggc aatatctacg ccctgatgta gccattgcac
     1201 tggtctgcac acttgattcc ctggtgccaa cccggctggc gatggcattt ctatcgcgga
     1261 ttgaagaata ctttcgttga catgggggca agtttccttt actttcgtat ttcgcaatga
     1321 atggcacact tacagctcgg gcgcacggtc accatcatca tcacggccac atgccgcgcc
     1381 acctgaggtg tgcatagcga ttcgctaaac aaagcactct tcagcccgct ggcgcaacaa
     1441 gccccagcgg gatttttatt ttcggtctca tagttttcag gagacgacac cgtggagatc
     1501 gactttcaga aaagcggcgc gctggtgccg gccatcatcc aggacgagcg caccggcgac
     1561 gtgttaatgc tcggcttcat gaatgcagag tcgctggcgg agacgcaacg caccggagaa
     1621 gtggtgttct tcagccgctc ccgaaacaag ctctggaaga agggcgaatc gagcggccac
     1681 gttttgcggg ttcgcgagat gcgcgtggat tgcgatgcgg atgcgctcct ggtgcgcgtg
     1741 gaagcggtcg ggccgggcgt ctgtcatgag ggttaccgga gttgtttctt ccgaaaacta
     1801 gaactggacg gaagcgttgc ggtcatcgct gagcgcacgt tcgcgccgga aaaagtttat
     1861 ggacaggagc aagctcgatg agcctgctga agctgggaat cccaaaaggc agcctgcagg
     1921 aggccacgct cgatcttttt tcgcgtgccg gctggaagat cacgctgggc tcgcgaagtt
     1981 acgtgccggc gattgacgac accgaaatcg agtgtttgct ggtgcgtgcg caggagatgg
     2041 cgcgttacgt cgagtccggc gcgctggacg cggggatcac gggccacgac tgggtggtgg
     2101 aaactggtgc ggcggtggac gaattgtcgg agctgattta tgcaaaacag cggctggcgc
     2161 gcgtccgttg ggttctggcc gtgccggaag actgcgcgat tcgggaaccg cgccatttgg
     2221 aaggaaaagt tatcgcgact gaggtcgtgc acatcacgga aaactatctc gctaagcatg
     2281 gggtaaaggc ccgcgtggag ttttcctggg gcgccaccga agtcaaagtg ccgcaacttg
     2341 cggacgcgat tgtcgaagtg acggagacag gctcgtccct ccgcgcgaat cgcttgcgca
     2401 tcgttgatac ggtgctggaa tccgcaacgg tgttcatcat gaaccgcgcc gcctcggccg
     2461 attcctggaa gcgcgaaaaa gcggaaaatc tgattctgat gctgcaaggc gcgattgccg
     2521 ccgcgagcaa agttggcttg ctcctgaacg ttcatcggga cgatctacag gcggtcctgg
     2581 gggttctccc tgcgctcaag aagccgacga tttccgcttt gagtgacccg gactgggtcg
     2641 ctatcaacac gatcgtcgag gaagcggtgg ttcggcagat tctgccaaag ctgaaagccg
     2701 ccaaagccca ggggatcgtc gaatatccct tgaacaagat tgtgctctga tgctcatcgt
     2761 gagacttacc ccggcggtcg agaggagact tctgcgcgca cgccaggagc acgacggaga
     2821 agcacagagg atcgcatcgg aaatcatcca tgatattcgc cggcgcggag acgccgcact
     2881 ttcggcttgg gtgaaaaaac tcgacgacat cgatctccaa cgcgaaagtc tgtggatctc
     2941 ggagggcgaa attcgtgtcg cccgcaagca agtgagccgc gagtttcttc gcgcgctgga
     3001 acacgccgcc cgcaatgtcc gccgcgtcgc ggaaaagcag atgccgaagc cctggacgat
     3061 ggaagtcgag cccggcgtga aaatcggcca gcgcgtgcgc ccgatcgatt ccatcggttg
     3121 ctacattccg ggcggacggt ttgctctcgt ttccaccatg gtgatgacgg tggtgcctgc
     3181 caaagttgcc ggcgtccgcc atatccgggt ggtctgcccg cggccgaacg ctgctcttct
     3241 ggccgccgcc ggcgttctcg gagtcacgcg tgtcgcccga atgggcggcg cacaagccat
     3301 cgcggctctg gcctacggca caaagcgcgt ggcccgcgtc gaaaaaatct tcgggccggg
     3361 caatcgcttc gttaccgccg ccaagcaact cgtcagcagc gattgtgcga tcgatctgcc
     3421 cgcggggcca accgaagcga ttgttttagc cacaacagga aacccgcgct ggatcgccgc
     3481 agacctgctc gcgcaggccg aacacgctcc cgatgcaggc agctatctcg tgacgacctc
     3541 aacaaggctg gcccggcaag tgcaaaccga agtggcgcgg caactgcacg aattgccgtc
     3601 gactaaccct gcgcaaatct cgatgaagcg cgcgggcgca attctggtcg cgccatccgt
     3661 ccatgccgcc tgcgaattcg tggatcgatt cgctccggaa cacctcagtc tacccggcaa
     3721 cggggagtca ctcttaaagc agattcacgc cacgggcacc atttttctag gaccttgggg
     3781 cgcgcaaccg cttggcgatt acgccagcgg aagcaatcac gtcctgccga ccggaggctg
     3841 ggcgcgcaag cgcggcggtt tatcggcggc ggactttgtg aagtgcatca gcgtacaatc
     3901 catcggccgc agtggcttcg cgcgattggc ggctgatgtc cagacgttgg cgcgcgcgga
     3961 aggtctgctg gcacatgcga acgccgtgga ggttcgccga tgaaagttca tttgccagtg
     4021 cgcaaagcga ttctgaaccg ccgcacgtat gaggcgcccg ccgaaggacg atccgataaa
     4081 ctgcgcctcg acttcaatga aaacaccgcg ggttgctcgc cagcggtgcg gcgcgcgctc
     4141 gcgaagctca ctactaaaca actggcgatg taccccgaat atcaggcgcc cacgcggcgg
     4201 atcgcgcgtt acttcggcgt ccgcccggaa gaattgctgc tcaccaatgg cggtgacgat
     4261 gccctgcgcg ttttcttcga taccttcgtg gagtccgaca gcagcatcct gatttgcgag
     4321 cccacctttc caatgtatcg ctactacgcg gagattttcg gcgcgcggat ttccgtccta
     4381 cattacggca gcgaaatgga attcccgctg aagggcgccg tcgccgcgct gcggaagaag
     4441 ccgcgcgttc tgttcatcgc caatcccaat aatccgacgg gaactctgct gcaaaaagaa
     4501 gagttgcgga gaatcttgaa agccgccacg cacaccgcgg tggtgatgga tgaagcttat
     4561 gcggagtttt caggctttac ggcggtgccg tgggttcgga agtatccgca gttgttcgtg
     4621 gcgaaaacgt tttcgaaagt tgcggggctc gcagccttgc gcctcggtgc ggtgatcgcc
     4681 tgcgaagagt cgctcgcgct ggtacggcgt gccatgccgc cctttcccgt gaatctcgcc
     4741 gctttggttg cgaccgaagc tgccgtggcc gatcgagcca caatgcaagg ttacgtgcgc
     4801 ggggtgaagc ggttacgcgc gtggttcgcc gaagaattgc agaagctcgg cgtgaaaact
     4861 taccgcagcg ccggaaattt tcttctggcg aatttcggga caagcgggct gcaactcttt
     4921 cagaagctgg agaagcaagg gattcttctc cgcgagaaga gcaaggacat cgggcccggc
     4981 tttgtgcgca tcaccatcgg aacgcaatcc gagatgcggc ggttgctgaa gacatttcga
     5041 aaggaatgga aggctccggc ttgaacgcca acgccaaaga gaaactgact atgccacgca
     5101 cagcgacgat tcatcgcaag acaaacgaga cggacatccg cctgaagctg catttagatg
     5161 gacgcggaaa ggcgcgcatt gccacgggaa tccgcttctt cgatcacatg ctcgagctgg
     5221 ttgcgcggca cggcgcattt gacctcgacg tggacgcgtg cggcgatctc gacgtggacc
     5281 agcatcacac agtcgaagat gttggcatca cactcggcga agcggtgcag aaagccctcg
     5341 gctcgaagcg tggcattttg cgcgccggct atttcctgat gccgatggac gaaacgctgg
     5401 ccgctgctgc ggttgatctt ggtggccggc cctattgtgt ggtgaatgca aaagtcgcag
     5461 ccaaacgcgt cggcgatttc caagtcgagc tgatggagga ttttttccag ggcttttcgc
     5521 aggccgcgaa ggccaacgtc catctgcgtg ctttgtacgg gcgctcgtca catcatcaag
     5581 tagaggctat gttcaaggcg ttcgcgcggg ctttgcgctt cgctgtgtcg cgcgacaaac
     5641 gcctgcggaa agttttgccc agcacaaagg gattgttatg aaagtgacga tcgtcgatta
     5701 cggcgcgggt aacgtgccct ccgtggaacg cgccttgcag cgtctcggag cagaatccga
     5761 gcggacgagt tcacccgaat gcatttcgaa agcggaggct ctgcttttgc ccggcgtggg
     5821 acattacgcc gctttggttc gcgctctcga tgaaaaaaca ctccgcgcac ctttggtcga
     5881 agccatccac cgcggtgtgc cttttctggg gatctgcctg gggctgcagg tgttgttcaa
     5941 gtcgagcgaa gaggcgtcac aactccaagg tttgaatctc cttcctggaa tcgtcagcgc
     6001 cttaccgccg ggcgtgaagt tgccgcacat gggctggaat cagctcatgg caaatcggga
     6061 ttcgtgcctg ctcgccggca ttgatgccgg agcgtatttt tattttgcgc actcctacgc
     6121 cgcgctcgat tcgaatggcg cggcagccgc tacctgctct tacggcgcgg aattcgccgc
     6181 cgtcatcgag caggagaata tctatgccgt gcagtttcat ccggaaaaga gcggtgatcc
     6241 cggcgctcgc gtcctgcaga attttctgag gcgcgcggca tgagcctcgc acaccgcatc
     6301 attccgtgcc tggacaccga cggtgagcgc gtcgtcaagg gagtcaactt cgccgggctg
     6361 cgcgatgccg gcgatcccgt cgcgctagcg gcgcgttaca acgcggaagg cgccgacgaa
     6421 ttggtggtgc tggacattgc cgcctcgcgc gaccggcgcc cgactttcct cgataccatc
     6481 cggcgcgtcg ccgcggaatt ggccatcccg ctcaccgccg gcggcggcat tcgaacgctc
     6541 gaagacggcc gcgccgtggt gcgcgccggc gcggacaaag tcaccgtgaa caccgccgcg
     6601 gtggaacggc ccgagctcat ttccgaattg tcccgagaat ttggcgcgca agccgtcgtc
     6661 ctggcgattg acgcgaagcg ctctggcgat cactgggatg tgatggtccg cggcgggcgg
     6721 gaatccaccg ctctcgatgc catcgagtgg gcgcggatgg gggtcgcagc aggcgctggt
     6781 gaaattctgc tcacctcagt ggatcgcgat ggcacgcaga gcggtttcga cgtagcgctg
     6841 accgcggcaa tctccagagc cgtaaccgtc cccgttatcg cgtccggtgg cgccaaatca
     6901 cccgaacatt ttgtcgaaat tttccaggag ggcgcggcgg acgcggcact agcggcttcg
     6961 atcttccacg atgaggtgca atccattcga tccctgaagg aattccttgc ggctcgcggc
     7021 gtcgaggtgc gcttgccgtg ctgattccct gcatcgattt gcagaatggc caagcggtcc
     7081 agttggtgca tgggcgcaga cgcgagctgg ctgtcgccga cgtcttcggt ttgttgcaga
     7141 aatttaaaaa atatccgtgg ctgcatgtag tcgatct
//
LOCUS       DASYVO010000130         2906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_103359, whole genome shotgun sequence.
ACCESSION   DASYVO010000130 DASYVO010000000
VERSION     DASYVO010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103359"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VGR03_04290"
     CDS             <1..927
                     /locus_tag="VGR03_04290"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEV1993530.1"
                     /translation="LHNRRLFAETFEKELNRARRYGQPLGLVTLDLHRFKEVNDKHGH
                     PRGDDVLRAAAATLKKALRTSDSAFRIGGDEFALLLPQTDSPQALALSRRVQTVFAEM
                     LQPMQLAVTVGMDHGVAIFPQDAEQADQLIRIADERLYQLKHANHGRSGNGSARATPP
                     AVPEAPAAPAQEQVSPAPQPISIETRRAPEKTEITIAAAASATSAAISGELTPAPSRV
                     FAVQRKAERVSMTGTNAYAVLGDQGARRARVLDLGFGGVAMELDSPEDLPENLLAVLH
                     VPILPPVRVNLKPVWKQQTGQGGFRVGCAFVS"
     gene            1052..1549
                     /locus_tag="VGR03_04295"
     CDS             1052..1549
                     /locus_tag="VGR03_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEV1993531.1"
                     /translation="MIEPKASRPPWPDAVQTPSKDLSGLKPWTWALERLEKSHNYWIS
                     TTRPDGGPHLMLVWGIWWQDAFWFSTGPRTRKAKNIAANPHVVIGTEKADEAVILEGI
                     VEEIKDRSVWKQLVEVYNRKYGGDVGPLLESSGGCIFRVTPQLAFGQDEHAENFTESV
                     TRWHF"
     gene            complement(1575..1997)
                     /locus_tag="VGR03_04300"
     CDS             complement(1575..1997)
                     /locus_tag="VGR03_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEV1993532.1"
                     /translation="MKARTTEDAGRKEIEAFNKRYVELHLKMDTQGILALWAEDGVDL
                     MPGDAVMIGRKNIVAWVEDIVAKMPGYKVTKQEMEFHDIRVCGDWASEWAAEHQVVQP
                     PDGKPPIDSYGKMALVLHREANGEWKVQQEMWNAAPKP"
     gene            2323..2571
                     /gene="rpmE"
                     /locus_tag="VGR03_04305"
     CDS             2323..2571
                     /gene="rpmE"
                     /locus_tag="VGR03_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEV1993533.1"
                     /translation="MKPGIHPDYHAVTVHCACGHNFSTRSTIKGDMLKVEICSNCHPF
                     FTGKQKLIDTAGRVERFTRKYADAAAKVEAAKVEAAKK"
BASE COUNT          576 a         1011 c          668 g          651 t
ORIGIN      
        1 ctccacaacc gccggctctt cgctgaaaca ttcgaaaagg aactcaatcg cgcgcgtcgc
       61 tacggccagc ctctgggatt ggttaccctc gacttgcacc gctttaagga agtaaatgac
      121 aagcacggcc atccgcgcgg tgatgacgta ttgcgcgctg ctgccgctac cttaaagaaa
      181 gccttgcgca catccgactc ggctttccgc atcggcggcg atgagttcgc tttattgctc
      241 ccgcaaacgg attccccgca ggcgctcgca ttgagccgtc gcgtccaaac cgtatttgcc
      301 gagatgctcc aaccgatgca gcttgctgtc accgtaggca tggatcacgg cgtcgcgatt
      361 ttcccccagg atgctgaaca ggcggaccag ctgattcgca tcgctgacga gcgtctatat
      421 caactaaaac atgcgaacca cggcaggtcc ggcaacggct ccgcgcgggc cacgcctccg
      481 gctgttcctg aagcgccagc agctcccgct caggagcagg tctcccccgc accgcagccg
      541 atttcgatcg aaaccaggcg cgcacctgaa aaaaccgaaa taaccatcgc ggcggctgcc
      601 tctgcaacct cagcggcgat ctccggcgaa ctcaccccgg ctccttcgcg cgtatttgcc
      661 gtccagcgca aggccgagcg cgtctccatg accggcacca acgcctacgc tgttcttggc
      721 gatcagggtg ctcgtcgcgc ccgcgtcctc gatcttggct ttggcggtgt cgccatggaa
      781 ctggattcgc ccgaagatct tccagagaat cttctggcgg tcttacatgt gcctattctt
      841 ccgcccgttc gcgtgaacct gaagcccgtc tggaaacaac agaccggtca gggcggcttc
      901 cgtgtcggat gcgccttcgt ttcctgaact tctctgcgta tttagctgac agcttcgacc
      961 cgccctcaaa aaagtactta gaacttctca gcttcacgct ttccgccctg atacaattcc
     1021 gccgcatctc cccgacccga aaggaggcgc catgatcgaa ccgaaagcca gccgacctcc
     1081 ttggcccgat gccgttcaaa ctccctcgaa agacctaagt ggtctcaagc cctggacctg
     1141 ggctctcgaa cgcctcgaaa aatctcacaa ctattggatt tccacgaccc gtcccgatgg
     1201 cggccctcat cttatgctcg tctggggcat ctggtggcag gacgctttct ggttcagcac
     1261 tggcccgcgc acccgcaaag caaagaacat cgccgcgaac cctcacgttg tcatcggcac
     1321 cgaaaaagcc gacgaagccg tcatcctcga aggaattgtc gaggaaatca aagatcgttc
     1381 cgtttggaag caactcgtcg aagtctacaa ccgcaaatac ggcggtgacg ttggccccct
     1441 tctcgaatct tccggcggct gcatcttccg tgtcactccg caactcgcct tcggccagga
     1501 cgaacacgcc gaaaacttca cggaatccgt cacccgctgg cacttctaat ccgtcgcgtt
     1561 ttcttgcgct actctcaggg tttcggcgcc gcgttccaca tctcctgctg aactttccac
     1621 tcgccgttgg cctcacgatg cagcaccagc gccatcttgc cgtagctatc gatcggcggc
     1681 ttcccgtccg gtggctgcac cacttgatgc tcggccgccc actccgaagc ccaatccccg
     1741 cacactcgaa tatcgtggaa ctccatctcc tgcttcgtca ctttgtatcc cggcatcttg
     1801 gccacgatgt cttcgaccca tgccacaatg ttcttccgcc cgatcatgac cgcgtcgcct
     1861 ggcattagat caacgccgtc ttccgcccac agcgccaaaa tcccctgtgt gtccatcttg
     1921 aggtgcaact ccacgtatcg cttgttgaac gcctcaatct cttttcgccc cgcgtcctct
     1981 gtcgttcgag ctttcattcc tacccccgcc atcagcaaag caacaaagca cgcctctaaa
     2041 agtacccttc ttatgaattc catccggccc ccttgatact cgcatcgatc cccgctcaat
     2101 ctaacgaaac ccgcccatcc ctgaaagctc aatctgcaac tactcaactc cttattctcc
     2161 atgccctatt gcctatcccc ttctccctat tccctattcc ctgtttggag gaatttgaca
     2221 cttcgcgtat gattaagaat tgcctcgcga cctagcgacg cggctgtagc ccccgaccct
     2281 gtcggttagc cgtgcagacc ggacgcgaca gaatggagcc tcatgaaacc cggtattcac
     2341 cctgactatc acgccgtaac ggtgcattgc gcctgcggcc acaatttctc gactcgttcg
     2401 accatcaagg gcgacatgct caaggtcgaa atctgctcga attgccatcc cttcttcacc
     2461 ggcaagcaga agctgattga taccgccggc cgtgtcgagc gctttacaag aaagtatgcg
     2521 gatgcagccg caaaagtgga agctgccaag gtcgaagccg ccaagaagta aaggtgcgca
     2581 agctcttgag ggacagccat tcctggctgt ccctctttct tttctcacaa ttcctctcgc
     2641 ttcctgtccc acaacttgtt gccctgcttc tctctctgcg cccctcagcg tacggcgcct
     2701 gccccgaccc ggtcggggcg cctctgcgtt attttttccc tttcaacttt taactttcga
     2761 ctttcaactt ccttccgctc cccgcccgtg tacaatgaac ttccaacgtg gctactcaat
     2821 cccgcatgtt tgatggcgtt cgccccggcg aactagtcga aaccgccccg ccgcgcgtca
     2881 ctctccgcaa ctacttcgcg caccca
//
LOCUS       DASYVO010000131         1626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_201756, whole genome shotgun sequence.
ACCESSION   DASYVO010000131 DASYVO010000000
VERSION     DASYVO010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201756"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /gene="coaBC"
                     /locus_tag="VGR03_04310"
     CDS             <1..1066
                     /gene="coaBC"
                     /locus_tag="VGR03_04310"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEV1993534.1"
                     /translation="MYDAAAGDPNLESAIEHIAVAQAIDLLVIAPATANVLAKMAQGM
                     ADDFLTTLCLATKAPLIVAPAMNVNMWENAATQKNLEVLRERGVKVLAPDEGYLACGM
                     MGAGRLTSIETIAQAVFAALGLRDDLKGETILVTAGPTEEPLDQVRFLSNRSSGKMGY
                     ALAEAAHRRGAGVILVSGPTHLDPPAGVQVERVRTAEEMSGAVRRNAGRAGIVLMAAA
                     VADFRPAEVPAQKIKKQNGVPEIKFEPTRDILADLASHRRADQVIVGFAAETEHVHEN
                     AAAKLRAKQLDLIVANDVTQKGAGFEVDTNIVTLIFPDGRQIPLQKMTKLEVAHRILD
                     QVAELRKKVAVREVLSTTKN"
     gene            1384..>1626
                     /locus_tag="VGR03_04315"
     CDS             1384..>1626
                     /locus_tag="VGR03_04315"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV1993535.1"
                     /translation="MFEAQPPVPTRRERETLEQIRADLGDCQRCRLAKTRKTIVFGQG
                     NPHAELIFVGEGPGADEDEQGLPFVGRAGKLLNRMMQ"
BASE COUNT          354 a          459 c          503 g          310 t
ORIGIN      
        1 aatgtatgac gccgctgcgg gcgacccgaa cctcgagagc gccatcgagc acattgcggt
       61 ggcacaggcg atcgacctgc tcgtgatcgc gcccgctacg gccaacgtcc tggcgaagat
      121 ggcgcaggga atggctgacg atttcctcac cactttgtgc ctggccacca aggctcccct
      181 gatcgttgcc ccggcgatga acgtgaatat gtgggaaaac gctgccacgc aaaaaaacct
      241 ggaagtgttg cgcgagcgcg gcgtcaaggt tcttgcgcct gatgaaggtt acctggcgtg
      301 tggcatgatg ggcgcgggcc gcttaaccag catcgagact attgcgcaag ctgtctttgc
      361 cgcgctgggc cttcgcgacg atctgaaggg tgagacgatc ctggtcactg ccgggccaac
      421 ggaggaacct ctggaccagg tgcgctttct cagtaatagg tcttccggca aaatgggtta
      481 tgcgttggca gaggccgccc atcgtcgcgg tgctggcgtt attctggtta gcgggccgac
      541 acatcttgat ccgccggcgg gcgtacaggt ggaacgagtg cgcaccgcgg aagaaatgtc
      601 cggggcggtg cggcgtaacg ctggccgtgc tggtattgtc ctgatggcgg cggcggtggc
      661 agactttcgt cctgccgaag ttcccgcgca gaagattaag aagcaaaacg gcgttccgga
      721 aattaagttt gaaccgacgc gcgatatcct ggctgacttg gcgagccatc ggcgcgcgga
      781 ccaagtgatc gtcggcttcg ccgcggagac ggagcacgtg catgaaaacg ccgctgccaa
      841 acttcgtgcc aagcaacttg atcttatcgt ggccaatgat gtcacccaga agggtgccgg
      901 cttcgaagtg gatacgaata tcgttaccct aatttttccc gatgggcggc agatccccct
      961 gcagaaaatg accaagctgg aggtcgcgca tcgcattctc gaccaggtcg ctgagctgcg
     1021 gaagaaagtt gctgtgcgcg aagtgctaag tacgaccaag aactgaaacg gcggcgaaag
     1081 gaaagtatca ggcacggtaa gagctgagaa cttcctccct caatggatga caaccttcgt
     1141 caagaattaa ccgcttggtt ggagttttac cgggagctcg gcatcgagag ctttcagaac
     1201 cacccgtcgg ccccgagtcc cgatgtgaag aaccctcgtt ccgagaatcg ggagagtccc
     1261 gctactcaaa acgagcccgc tccggcgtcc gcgccgagcc tggccgcgca aagagcggca
     1321 attgaaactg cccgcccgac catccaaatt cctaaaccta tggtggaaat ccccaagtta
     1381 aatttgttcg aagctcagcc cccggtccct acccgacgcg agcgcgaaac gttggagcag
     1441 attcgcgccg acctgggcga ttgccagcgt tgccgcctgg caaaaactcg caagacgatt
     1501 gtgtttggcc agggcaatcc gcacgccgaa ctgattttcg tgggtgaagg tcccggggcg
     1561 gacgaagacg agcaaggatt gccgtttgtg gggcgcgccg gcaaactttt gaaccgcatg
     1621 atgcag
//
LOCUS       DASYVO010000132         3457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_46916, whole genome shotgun sequence.
ACCESSION   DASYVO010000132 DASYVO010000000
VERSION     DASYVO010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46916"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VGR03_04320"
     CDS             <1..307
                     /locus_tag="VGR03_04320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1993536.1"
                     /translation="AAGLAGCVYVEIRGDRGYLGLLGVDPQRQGTGLGRKLMDAAENF
                     FREAGCLAADLRIVSARTLLPAFYRHLGYLETGTAPFASDVPAKVPCHYILMSKIIA"
     gene            437..2719
                     /locus_tag="VGR03_04325"
     CDS             437..2719
                     /locus_tag="VGR03_04325"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEV1993537.1"
                     /translation="MNRTIAIASILFVGISLAPVGNHVATDEQPLFGYSAESSRTERQ
                     WEEKLRAIPSPDNLRAYMQRLSAHPHHVGSPYDKDNAEWIAAKFKEFGLDTHIEQFDV
                     LFPTPKERVVELVEGGPKFVAKLQEPALPQDPTSNQQAEQLPTYNAYSIDGDVTAPLV
                     YVNYGIPEDYEQLERMGISVKGKIVIARYYHSWRGIKPKVAAEHGAIGCLIYSDPHED
                     GFVQGETYPAGAYRPPDGVQRGSVADMPFYPGDPLTPGVGATKDAKRLKVEDAATITK
                     IPVLPISYSDAQPLLAALTGRVAPEGWRGGLGITYHIGPGPAKVHLKVKSNWDIKPIY
                     DVIGKIPGATFPDEWVIRGNHHDGWVNGAEDPISGQVALMEEARALGELVKSGWKPKR
                     TIIYCAWDGEEPGLLGSTEWAEQHYDELRAHAVAYINSDGNGRGYLGIEGSHTLEKFS
                     NDIAREISDPETKLSVWKRQQLHDIANAKTPEQRDEIRKRADLRIPALGSGSDYTAFL
                     QHDGVASLNIGFGGEDDGGIYHSIYDDFYWYTHFSDTDFVYGRALAQTGGTAVMRLAD
                     ADLLPFEFGNFADTVQTYLKELKTLSQKMQDDIRERNKEIEEGVFNGTNDPKRPLVAP
                     SAEAVPPHLNFAPFENAVEVLSRSAADYRKALEQANANGGAVLASASLVEVNKMLIDS
                     ERKLTNAEGLPNRPWFKHQLYAPGFYTGYGVKTVPAVREAIELKKWKQADEAIVIVAR
                     MLQDEAALISSAAQKLSGAK"
     gene            complement(2780..>3457)
                     /locus_tag="VGR03_04330"
     CDS             complement(2780..>3457)
                     /locus_tag="VGR03_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEV1993538.1"
                     /translation="PCFSMRCFVPETIPPTERTRVVREAHRGAYDRETIDKILDEGYV
                     CHVGFAVDGQPYVIPTLFARIGDAIYFHGSAASRMLRNVSGGISVCLTVTLTDGFVLA
                     RSVFNHSMNYRSVVALGKATLVEQPEEKLEALRAFTEKIIPGRWNDARQPNEKELKST
                     SILRLPLTEVSAKIRSGPVEDDAPDYALPVWAGVIPLSLTPGAPIRDERCDPSIPTPA
                     YAAQYKR"
BASE COUNT          742 a         1067 c          978 g          670 t
ORIGIN      
        1 cgcggctggc ctcgcaggat gcgtctacgt ggagattcgc ggcgaccgtg gctacctcgg
       61 cctacttggt gtggacccgc aacgccaggg caccggcctg ggccgcaaat taatggatgc
      121 cgcggagaat tttttccgcg aggccggctg cctcgcggcc gaccttcgca tcgtcagcgc
      181 ccggacgctc cttcctgctt tctatcgcca cctcggctac ctcgaaaccg gcaccgctcc
      241 tttcgcttcg gacgtgcctg ccaaagttcc ctgccactac atcctcatgt ccaaaatcat
      301 tgcctgaaat tttcgtaaca ccatgcagag gaagccttta tctaagctgt cgcctatggt
      361 tatccgaata accggattct ctcattccca atccttccgc cttcaggcag aatacccgtt
      421 ccattctggg ggaaccatga atcgcaccat cgcaatcgca tccattctct tcgtgggaat
      481 ctcgctcgcg cccgtcggca atcacgtcgc cacggacgag cagcctctct tcggctactc
      541 cgccgaatct tcccgcaccg agcgccagtg ggaagagaag ctccgcgcca ttcccagtcc
      601 cgacaatctc cgcgcttaca tgcagcgcct atccgcgcat ccacaccacg tcggttcgcc
      661 ttacgataag gacaacgccg aatggatcgc cgcaaaattc aaggagttcg gtctcgatac
      721 gcacatcgag cagtttgacg ttctctttcc aacgcccaag gaacgcgtcg ttgagctcgt
      781 cgaaggcggt cccaagtttg tcgccaagct ccaggaaccg gcacttccgc aggaccccac
      841 ctccaatcag caagccgagc agcttcccac ctataacgct tactcgatcg acggcgacgt
      901 gaccgctcct ctcgtctacg tcaactacgg catccccgag gactacgagc agctcgagcg
      961 catgggcatt tccgtgaagg gcaagattgt catcgcgcgc tactaccact cctggcgcgg
     1021 tatcaagccc aaggttgcag ccgagcacgg cgcgattggc tgcctgattt actccgaccc
     1081 ccatgaggac gggtttgtcc aaggagagac ctatcccgcc ggagcctatc gcccaccgga
     1141 tggagtccag cgcggcagcg tggcggacat gccgttttat ccgggtgacc cgctgacgcc
     1201 gggtgttggg gcaacgaaag acgccaagcg gctgaaggtg gaagatgctg ccacgatcac
     1261 gaaaattccg gtcctgccaa tctcctattc cgatgcgcag ccattgcttg cggcgctgac
     1321 cggccgcgtt gctccggaag gatggcgcgg cggcctcggt atcacctatc acattggccc
     1381 cggccccgcg aaagttcatc tcaaagtgaa atcgaactgg gacatcaagc ctatttacga
     1441 cgtcatcgga aaaatcccag gcgcaacgtt tcccgatgag tgggtgattc gcggcaacca
     1501 tcatgacggg tgggtgaacg gcgcggagga tcccatctca ggacaagttg ccctcatgga
     1561 agaagcgcgc gcgctcggtg agctggtcaa gtccggctgg aagcccaagc gcaccatcat
     1621 ttattgcgcg tgggatggag aagagccggg actgctgggc tcgaccgagt gggccgagca
     1681 acactacgac gaattgcgcg cgcacgctgt cgcgtatatc aattcagacg gcaatggccg
     1741 cggttatctc ggcatcgaag gctcgcacac gcttgaaaaa ttcagcaacg atatcgcgcg
     1801 cgaaatatcc gacccggaaa caaaattaag tgtctggaag cgccagcagc tccacgacat
     1861 cgccaatgcc aaaaccccgg agcagcgcga tgaaattcgc aagcgcgccg atctgcgcat
     1921 tcccgcactc ggctccggct cggactacac cgcattcctg cagcacgacg gcgtggcttc
     1981 gctcaacatc ggattcggcg gcgaagatga cggtggcatc taccattcca tctacgacga
     2041 tttctactgg tacacgcatt tcagcgacac ggattttgtc tacggccgcg cgctcgcgca
     2101 gaccggcggc acggccgtga tgcgcctggc ggacgcggat ttgcttcctt tcgagttcgg
     2161 aaattttgcg gataccgtgc agacctactt gaaggaactg aaaactcttt cgcagaaaat
     2221 gcaggacgac attcgtgaac gaaacaagga aatcgaagaa ggcgttttca atggcaccaa
     2281 cgacccgaag cgcccgctgg tcgcgccttc cgcggaggcg gtgccgccgc acctgaattt
     2341 cgcgcccttc gagaacgctg tggaagtgct ctctcgcagc gcggcggact atcgcaaagc
     2401 gctggaacaa gcaaacgcca atggcggcgc tgtgctggct tccgcgtcgc tcgtcgaagt
     2461 caacaagatg ctcatcgaca gcgagcgaaa actcaccaat gccgagggcc tccccaaccg
     2521 gccgtggttc aagcaccagc tctacgcccc gggcttttac accggctatg gggtcaagac
     2581 cgtgccagct gtacgcgaag ccatcgagct aaaaaaatgg aagcaggccg acgaagccat
     2641 cgtcatcgtc gcgcgtatgt tgcaggatga ggccgcgcta atttcatccg cggcgcagaa
     2701 actctcagga gcgaaatgaa gttttctcga aaaagcgatg ccgccatgtc caatcggctc
     2761 gcgcgccgtt cagcgcagtt caccgcttgt actgcgccgc gtacgccggc gtgggaatcg
     2821 aaggatcgca ccgctcatca cggatcggcg cgcccggcgt gagggaaaga ggaatcacgc
     2881 cggcccagac cggcagcgcg tagtccgggg cgtcgtcttc gaccgggccg cttcgtatct
     2941 tggcggaaac ttccgtgagt ggtaggcgca gaatgctcgt ggatttcagt tccttttcat
     3001 ttggctgacg ggcatcgttc cagcggccag gaattatttt ttcagtgaat gcacgtagcg
     3061 cttcgagttt ttcttccggc tgttcgacga gagtagcttt tccgagtgcg acgacggaac
     3121 ggtaattcat ggagtgattg aataccgagc gcgcgagaac gaagccatcc gtgagagtca
     3181 cggtgaggca aacagagatt ccaccgctca cgttccgaag catgcggctt gcggcggagc
     3241 catgaaaata gatcgcgtcg ccaatgcgcg caaacagcgt ggggataacg taaggctggc
     3301 catcgacggc gaatccgacg tggcacacgt aaccttcgtc gagaattttg tctatggttt
     3361 cgcgatcgta agcgccgcgg tgcgcttcgc gcaccacgcg cgtacgctcg gttggcggga
     3421 tagtttcggg cacgaagcac ctcatggaaa aacatgg
//
LOCUS       DASYVO010000133        23221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_172591, whole genome shotgun sequence.
ACCESSION   DASYVO010000133 DASYVO010000000
VERSION     DASYVO010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 23221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23221
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172591"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VGR03_04335"
     CDS             <1..286
                     /locus_tag="VGR03_04335"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEV1993539.1"
                     /translation="PERRSSMRKALAIPVRVHPERIPWFEEAMTIDISAKGMRFRSHR
                     EYELGDHLKIAFQDPASAPWHGASEFRSKVIRIAPVPGSIALDVSVCRVE"
     gene            364..1023
                     /locus_tag="VGR03_04340"
     CDS             364..1023
                     /locus_tag="VGR03_04340"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEV1993540.1"
                     /translation="MTSTHDSRNLFLEGPAGRLEAILWTSSAAAHPPLAAVVCHPHPL
                     FGGTMHNKVIYQAAKSLDSFGIPVLRFNFRGAGLSAGVHDRGLGEQDDVRAALDFLAS
                     EFPGVPLLLAGFSFGAWVGMRVGCEDPRVSHLIGLGIPVNSTDFSFLLQCNKPKLFVH
                     GSNDEHGAVEKVKALIPTLPGGNHLVVVEGVDHFFAGKLDQLDRAINAWFTDEVFRLR
                     H"
     gene            complement(1100..2212)
                     /locus_tag="VGR03_04345"
     CDS             complement(1100..2212)
                     /locus_tag="VGR03_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV1993541.1"
                     /translation="MMSKSATKEGTTRYAQKFAGRAAAGHFRETQRLVLSSLGIGTYL
                     GQPDAKTDEGYAAAAVAAVESGINVMDAAINYRFQRSERSIGAAIRQLAAKGFEREEI
                     VVCTKGGYLTPDGAMPGDPNEYFFREYIQPGVFTAKDIAAGSHCMTPKFLKNQIGRSL
                     KNLGVECVDVYYLHNPETQLSEVTKEEFLSRVREAFTFLESAVEAGEIQYYGMATWNG
                     FRQDARARDAMQLGEISQIAQEIAGGRHHFRFVQVPFNLGMTEALTLGNQSLRGHHRT
                     IMEAASELDITLIASASLLQGQVAKNLPEFVAEALGLGNDAERALQFVRSSPGITTAL
                     VGMSRAEHVKANARLVGVAPATAEQFSKLFSRREGA"
     gene            complement(2209..3390)
                     /locus_tag="VGR03_04350"
     CDS             complement(2209..3390)
                     /locus_tag="VGR03_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993542.1"
                     /translation="MLELDCGLEFDAAGAEEHFFPALPPRAAVCLIEAREENAEPFLI
                     RTQNLQRRLQRLLGPIDPASKRLNLSDFARGIRYRLIGSPFEQTLTYYQHAKLLFPER
                     YRDLMRLRPAAVLKLNLRNAYPRCYVTRRIPVDEAGVPTGGAYYGPFPSRRFAQAFAE
                     RILDLFKVRRCQIKIRRDPTFPGCLYSEMKMCLAPCFAGCTKEEYDVEVQRLVQFLET
                     SGGSLRAIIQEEREKASEQLDFERAAALHKKVEKLDEVLRGRPELTRRIQDLDAVILQ
                     RAAEEQTIGVYGVRGGRLAEPFFLRFAEIASQPRSAEQIFREHFEAEPTTTNGDLGEH
                     LWLVARWYYSNPREGEIFFREKDWPYRRILRACSRLLAPKTNEVETNPTPGPAQPPEG
                     A"
     gene            complement(3483..4625)
                     /locus_tag="VGR03_04355"
     CDS             complement(3483..4625)
                     /locus_tag="VGR03_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEV1993543.1"
                     /translation="MDLELTEEQQLLQKSVREFAESEVRPLAKELDETGHFPRALFKK
                     AAELGLTGVSFPESEGGAGFDHIAYSIVIEEISRCCASTGVILSVQNSLYCDPIHRYG
                     TEEQKKKFLLPYARGEKIGCYALTEPQAGSNAAALQTKAVKKGDTYVINGTKAWITNG
                     GAADAAIVYANTEPAKGEKGITALVVERGTPGFKVGKEEKKLGISATACSELIFSDCE
                     VPVANRIGNEGEGYKVALSTLDGGRIGIAAQATGIAQGAFEAALKYSQERMAFGHPIS
                     QFQAIQFMLADMSTEIDAARLLIRKAAWKQDTGARFTMDAAIAKLFASEMATRVTHKA
                     IQIHGGNGYSREYPVERNYRDARITEVYEGTSEIQRLVISSWVLKS"
     gene            complement(4758..5243)
                     /locus_tag="VGR03_04360"
     CDS             complement(4758..5243)
                     /locus_tag="VGR03_04360"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEV1993544.1"
                     /translation="MDYIWTPWRYQYMAQVSGGKQPECIFCDAVSRKNDAETLIVHRG
                     EKTFVILNRYPYTSGHVMIVPYAHVAELNLCDAGTLGEIMQLAQRVETALSANYKPDG
                     MNLGMNLGRAAGAGVVGHLHLHALPRWIGDSNFMTVTCETRVHPEDLKTTYERLSKAL
                     G"
     gene            complement(5416..5742)
                     /locus_tag="VGR03_04365"
     CDS             complement(5416..5742)
                     /locus_tag="VGR03_04365"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV1993545.1"
                     /translation="MTKQPTLTKADLIEEVLRITELPRKESETIVETIFDSIIGSLQK
                     GQKIEIRGFGSFRTRERRGRVGRNPKTGAKVEVPAKKIPFFKPSKELKDFVNSSEAQA
                     APAGGE"
     gene            complement(6024..8000)
                     /locus_tag="VGR03_04370"
     CDS             complement(6024..8000)
                     /locus_tag="VGR03_04370"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV1993546.1"
                     /translation="MFSDHDNQSNAAAAAAAPEGETAPNSAHTETNSTVESATEQTAP
                     PAATEHTEAVNTSQETPAVAAAEQAKPVAETHAADPEAAAAAEEAAGSEEMSKLLEEY
                     DEKQEAAASHEVIEVKVVAYTEHGVVVDLGGKTEGLIPAAEFTDTEIPRPEPNSTIEV
                     QRTGEHKDGFTILSYLKVLRRKTWEKIEAAFKTKETITGKVVDRIKGGLVVDIGVRAF
                     LPGSQYDLRPAQNLDNLAGTEIQVRITKLNRRRGNVVVSRRALLEEELHAKRATLMET
                     LSEGQVVHGHVKNVTEYGAFVDLGGIDGLLHLTDLSWGRVKHPSDVVKPEQEVDVIIL
                     KFDKEKQRVSLGMKQLMADPWVGAAEKYPAGGKVKGKIVGVVDYGVFVELEQGIEGLV
                     HVSEMSWNKKIQHPSKVAKVGEEVDVVVLDIKPSDRRVSLGIKQALPDPWLVIAEKYP
                     VGMVITGKVRNIAEFGAFIEIEDGFDGLVHVSDVSWTERIKNPHEVFKKGDDITAKVL
                     KIDPENRRVSLGIKQVNDIWGDWFKQHKVGQIVKGKVSRIATFGAFVELGENIEALCH
                     NTEIEERKRRDDGPSSFRPTTGPLKSAGPLEPGKEYDFKIVKISPETRRIGLSYRAAV
                     KQIERKEIEQYRSTSKTSSTATIGDALKSKLSAR"
     gene            complement(8280..9080)
                     /locus_tag="VGR03_04375"
     CDS             complement(8280..9080)
                     /locus_tag="VGR03_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1993547.1"
                     /translation="MALENCTLCRGTGWKLVPRGDGATGSVAVACDCGMEERASRVME
                     RARIPKRYEHCDFESYVTDLTDGKTWTAQHAQSLKQAKLFTQGFVREYPGSSEKGLLF
                     MGPSGVGKTHLAVAALKELIHRGHAGLFCDYRELLKEIQASYNPASESTEMKILEPIR
                     TVEILVLDDLGASKPSDWVRDIVGIVLNARYNENRTTIITTNYLDNPATEGEAARLPN
                     GKLIAPTREDALEQRIGSRMRSRLYEMCRTVEVSAPDFRREKTHAGRA"
     gene            complement(9309..9698)
                     /locus_tag="VGR03_04380"
     CDS             complement(9309..9698)
                     /locus_tag="VGR03_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1993548.1"
                     /translation="MPPRAKKRAKAGYMISAVAELYKLHPQTLRLYERVGLLKPSRSQ
                     GNTRLYTDSDLERLDVILTLARDMGVNLAGIEIILNMREKMIEMERQMGEFAHVVQQE
                     LSRVAASHMQEKTPGHAIVRATPRLVR"
     gene            complement(9709..10872)
                     /locus_tag="VGR03_04385"
     CDS             complement(9709..10872)
                     /locus_tag="VGR03_04385"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEV1993549.1"
                     /translation="MPTTAKQDYYELLGVARKAGAKDIRAAFRKLARKYHPDLNPGDK
                     SAEEKFKQLQEAYDVLSDSKKRQMYDQYGFYSDNLPPGGRGGAGGHGGEPDVNFDFGG
                     FDFGGGSGAAGGGSSFRDLFSQFFSGGRGSAGMEPEHEPGGDLEYQIEIDFWDAVRGA
                     VKKLSITRMDACETCHGTGAVGSPQICTACAGTGTIQQAAGKMRFNVPCTRCGGTGKL
                     RTLCKTCSGEGRVRRTESIEVRTPAGVSNGGRVRVPGKGNSGTMGAPAGDLYLRVVVK
                     PHEFFERRGDDLYTKIPVTVSEATLGAKIEVPTMDGRSLVRIPPGTNSGKTLRLKEKG
                     VPSARNGARGDQYVEIQVVVPPPTDERVRNLMKELESVAPGDPRKDLFSKAGV"
     gene            complement(10972..12930)
                     /gene="dnaK"
                     /locus_tag="VGR03_04390"
     CDS             complement(10972..12930)
                     /gene="dnaK"
                     /locus_tag="VGR03_04390"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV1993550.1"
                     /translation="MSKIIGIDLGTTNSVVAVMQGGEPVVIPNQEGARTTPSVVAITK
                     TGERLVGQVAKRQAVTNPENTVYSIKRFMGRRFDEVGEEMRRIPYKVVKGDHDDARVE
                     IFDKVYSPPQISAMILGKLKSAAEDFLGEKVTKAVITVPAYFNDAQRQATKQAGEIAG
                     LEVVRIINEPTAAALAYGLDKKNEETIAVYDLGGGTFDISVLEVGDMVVHVKSTNGDT
                     HLGGDDVDQKIVDWLIDEFKKKNGIDMSKDKMALQRLKDAAEKAKIELSQMMETEINL
                     PFITAGANGPIHMEEKLTRTRLEQMMNDLLERSMKPVKQALEDAKMSPNDIQEVVLVG
                     GSTRIPKVQSLVRTFFGGKEPHKGVNPDEVVAVGAAIQGAVLAGEVKDILLLDVTPLS
                     LGVETLGGVMTVQIPRNTTIPTRKVETYSTAADNQPGVEIHVMQGERKFATDNRSLGK
                     FKLDGIPPAPRGTPQIEVTFDIDANGILNVSAKDKATSKEQKITITASSGLTKEEAEK
                     MRTEAESHSEDDRRRMEEVEARNRLDGILYQSEKMLRENREKIAEADVKKAEEAIEDA
                     KKAINEGGVARLRSATENVERSLHKIAEELYKTTQAAGGPEHAGAASAGAGPGGSSAG
                     GPSGAQEKKPGDVIDAEYVDVDESKRPN"
     gene            13651..14433
                     /locus_tag="VGR03_04395"
     CDS             13651..14433
                     /locus_tag="VGR03_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEV1993551.1"
                     /translation="MNRRRILAGILSVGLLGLIALFLPSLHKSPEPASPSSQPLAQSP
                     SESLGSNAEAEPTVIPTSVVAAKAASRPPAPQSAAGLPDVDPHKAFGSKNAPVTMEVF
                     SDFQCPACKTLFTTTNRRLMDDYVSSGKVYLIHRDFPLPMHAHSRVAARYARAAAQLG
                     KGEPVEQALFQNQEKWEQNGDVDGTVAAILSAAEMAKVRALVKGGTLDPMIDKDYALG
                     QTYRVNQTPTTVFHCKGQTYPYSGVMTYDILKTFLEQLLSQK"
     gene            14434..14934
                     /locus_tag="VGR03_04400"
     CDS             14434..14934
                     /locus_tag="VGR03_04400"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEV1993552.1"
                     /translation="MMQSSPLNLRRAIIWIGRLVLAGIFIYAGYAKLTMGMHPRPPIR
                     VALSFFALQVDSYQILPSGGVKFVANTLPFAEIGLGLLLLLGWRFRIWATLVTLIMLG
                     FFAAVVRSYALGLQINCGCFATPEPLTIKTVFRDGALVALALLMTIFAFMEARKPHPW
                     SAPEKA"
     gene            14950..15528
                     /locus_tag="VGR03_04405"
     CDS             14950..15528
                     /locus_tag="VGR03_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HEV1993553.1"
                     /translation="MKTLAEYLDLAAVAHGHLCAGQVLGVRLAMLGLRELGIVDPVAE
                     RKRIVTYVEIDRCVTDAVALVANCRLGKRALKFRDWGKVAATFVDLQTGRAVRIAAKE
                     SSKQAAREMFPELGKDAGQQKAYAELSDEILFDKQWVKVEVQPEDLPGFKGPRVVCAQ
                     CGEGINFKREVVKDGSTLCRACSGEKYYETLS"
     gene            15584..17890
                     /locus_tag="VGR03_04410"
     CDS             15584..17890
                     /locus_tag="VGR03_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEV1993554.1"
                     /translation="MPPITLRLRGWKLIDRVALAFLILCAVVLGAGCGLLFVYASDLP
                     EIRALETYRPDVVTEVYADDGQLAGSFALQRRILMTYEQCPKVLYSAVTAIEDQHFEE
                     HWGIDFPRMASAAVRNLIKRKITGGASTITMQLAGNLFLDRSDRSFRRKMQEILLALQ
                     IERRYTKPQIFTMYANQVYLAHGNYGFAAAAQFYFGKPVTDLKLQEAALLAGMVNGPK
                     FSPLTNPELALNRRNLVLHRMEEEGKITPTEETTNRKSPLGLHIQYPRNDLAPYFFEE
                     IRKYLESTYGSEAVHERGLRVYTTLNIKMQRIANQSLRDGLHNYERRHGWKGNLPNVL
                     RDNLGKLDTYEDDDWRHAIEKGSYVTGLVLTVSDRDAVIKIGTYRGILSASEFGWTGR
                     KKPSDLLKVGDLAQFSIQELRDNTVRVQLEQQPGPQGALLAIDNSTGEIKTMIGGYSF
                     EDSKFNRATQAVRQVGSSFKIYVYADALERGATPFDTIVDLPFTTISGGQPYSPRNYD
                     EKFEGTITLRRALAGSRNVPAVKLAEKIGINTVVDMTRRFGITTPLPPYLPLALGAAD
                     MKLLEHVSAFTVFPNDGIRIDPHMIRRVTSYDGALLEEAHPEVHDVLSPDVARTMTAM
                     LEEVIRFGTGIQAKALGRPAAGKTGTTQDYTDAWFVGYTPQLTTGVWVGFDDKQISLG
                     KKETGARAALPIWLEFMQGAVAGTPAMDFPNVVPLEQQAGEHHVSVDTPDTAPTEDEP
                     AAPKEKPPAVLPPTRAGAVGATGVSQRR"
     gene            complement(17932..18759)
                     /locus_tag="VGR03_04415"
     CDS             complement(17932..18759)
                     /locus_tag="VGR03_04415"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HEV1993555.1"
                     /translation="MRKLFCVLAFVTALCFPKGVLAQQLIRVNCGGPSYTDSKGQVWQ
                     ADSGFTGGSEETISGSVAGTSDPLLYEDYRWNPTSYSFPVSNGAYQVNLYFTEAAPKA
                     ESVGARVFNVSLQGEMVFANLDIYAAAGANAALIKSANVNVTAGAINIAFAHVSGLNP
                     KISAIEILPLPATSPVLVLNFKYPDGTPVAGTINYSISSSLLSFQGSAPLINGQAQCV
                     LFSNPSALGISAQFTVNLNLTDSTGHQLWQLSLGMNPAQVNLGAIQTSSLNVVVQKL"
     gene            complement(18993..20003)
                     /gene="thiL"
                     /locus_tag="VGR03_04420"
     CDS             complement(18993..20003)
                     /gene="thiL"
                     /locus_tag="VGR03_04420"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEV1993556.1"
                     /translation="MPRKRQSEDSILRKIADSSRSSQVPRAGHSVALGIGDDAALFRP
                     KPGHETILTCDWFLEGTHFLREKHPPDAVGWKCLARGVSDIAAMGGTPRCFLLSLALP
                     ETHTGRWLDLFLGGLQRASRKFQCALAGGDTTRRNEILINVTVVGEVPAGRAGLRSGA
                     RAGDILYVSGRLGEAELGLRIVRRSKGAASRKNQLTRKHLYPEPRLALGQWLVKKGLA
                     TAMMDLSDGLSSDLPRLCAASAVGALLDWANIPQVQIPDVALIHGRDPMQLALHGGDD
                     YELLFTVPPRKAKLLPKTFRGVGLSAIGRITRDRALLVLEASGHARQLLPSGWDPFRK
                     KL"
     gene            20322..21203
                     /locus_tag="VGR03_04425"
     CDS             20322..21203
                     /locus_tag="VGR03_04425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEV1993557.1"
                     /translation="MRGKSYTFYIASSPGKLKKLIVPAYVLHGIAVLALIGGITVTAA
                     VGSYSRMLWKVGNYNALRHDQETLKKQYQQLQSTVNNTNQRLDSLQSLATEVAMTYGV
                     LRYHPAAFDQSDNPVDAQDAFDRSIEQYTFLKRNAAVIAISSSGLHLLSPMSFADSTY
                     TPAIWPVMGHITDSFGERLDPFSGEGTFHTGVDVASDYGAPVHATADGIITIAENHAG
                     YGRLVVIDHGFGITTWYAHLSAFSAVSGARVKRGEVVGYTGISGRSTGPHVHYEVRMN
                     NAPINPWRYMKSTTAGD"
     gene            complement(21319..22395)
                     /locus_tag="VGR03_04430"
     CDS             complement(21319..22395)
                     /locus_tag="VGR03_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993558.1"
                     /translation="MTKRTWIGLAAVVVFLSAAAFFGYRKWSVQNGSARGEMLTLMPS
                     DASAILFVDFGELRQSPFIAQLYAWAPKPQADADYAQFVKETGFDYERDLHRVAIAVE
                     RRGQDSPLFAVLDGKFDRQKISAYALRDGSAVKTAGREIFSVPVSGTAKKISFTFLRN
                     DRIALTNDANLAVFLDAKKRSEDVSEWRARFERLAGSPVFAVIRQDAAIGAALAAQAP
                     GGLRSPQLSSLLNQLQWITLAGKPENDRLRVVAEGECTAEATVRQLVDVMNGVVILAQ
                     GGLNDARTRQQLDPAMREAYLELLKSADVSKIDRGDTKSVRLVFEITPGFLEAARKAP
                     PVMPDSAPGKPLPGKAPAPRKGHT"
     gene            22703..22778
                     /locus_tag="VGR03_04435"
     tRNA            22703..22778
                     /locus_tag="VGR03_04435"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22736..22738,aa:Thr,seq:tgt)
     gene            22978..23066
                     /locus_tag="VGR03_04440"
     tRNA            22978..23066
                     /locus_tag="VGR03_04440"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23012..23014,aa:Tyr,seq:gta)
     gene            complement(23035..>23221)
                     /locus_tag="VGR03_04445"
     CDS             complement(23035..>23221)
                     /locus_tag="VGR03_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993559.1"
                     /translation="PLKNHPSRRTFPERRRFFRCDRKSVGAPNQTAFEHRLMRLPCGF
                     LQPHIESLVDGEGFEPS"
BASE COUNT         4349 a         7467 c         6178 g         5227 t
ORIGIN      
        1 acccgaacgg cgcagcagca tgcggaaggc gctggcgatt cccgtccgcg tgcatcccga
       61 gcggattccc tggtttgaag aagccatgac tatcgacatt tcggcaaagg gcatgcgctt
      121 tcgcagtcac cgcgagtacg aactcgggga tcacttgaaa atcgcgtttc aagacccggc
      181 ttctgcgcca tggcatggcg ctagtgagtt tcgctcgaag gttatacgca tcgctcccgt
      241 gcccgggagt attgccctcg acgttagtgt ttgccgtgtg gaataaacga atcgagaatt
      301 gccagccgca ttcggtcttt ttccttcgat gcgctaaact gcaaattcgt ccatggcgca
      361 tcgatgacca gcacccacga ctcccgcaat ctctttttgg aaggacctgc cggacgcctc
      421 gaagcgattc tctggacatc gtccgccgcc gcgcatccgc cgctcgccgc ggttgtctgc
      481 cacccgcatc cgctctttgg cggtacgatg cacaacaaag tgatttacca ggcggcgaaa
      541 tcgctggatt ccttcggcat tcctgttttg cgattcaatt ttcgcggcgc aggactcagc
      601 gccggcgtac acgatcgcgg tctgggcgaa caggatgatg tccgggccgc gctggacttt
      661 cttgcttccg agtttcctgg tgtgcccctg ctgctcgcgg gattcagttt cggcgcctgg
      721 gttggcatgc gtgtcggctg cgaggacccg cgcgtctctc acctgatcgg cttgggtatt
      781 cccgtcaaca gcaccgattt ctcattcctg ctccagtgca acaagcccaa gttattcgtg
      841 catggaagca acgacgaaca cggcgcggtc gaaaaagtca aggcgctcat ccccacgctc
      901 cccggcggca accatctcgt tgtcgtcgag ggagtcgatc atttctttgc cggcaaactt
      961 gaccaactgg atcgcgcaat caacgcctgg tttaccgacg aggtttttcg tcttcgccat
     1021 tagatctcgt ggatttccgt ttcttttctt tctccgtgaa ctatgcgtcc tctgcgtcct
     1081 ctgtgttaag tccttttctt caagccccct cgcggcgcga aaaaagtttg ctgaactgct
     1141 ctgccgtcgc cggcgcaacg cccaccagcc gtgcgttggc tttcacgtgc tccgcgcgag
     1201 acattcccac cagcgcggtc gtaattcccg gtgaggaccg cacgaattgc agcgcccgtt
     1261 ccgcgtcgtt cccgagacca agcgcttccg cgacaaactc cggcagattc ttcgccactt
     1321 gaccttgcag cagcgacgcg ctggcgatca acgtgatgtc cagttcgctc gccgcctcca
     1381 tgatcgttcg atgatgcccg cgcagcgatt gattgcccaa cgtcagcgcc tccgtcatcc
     1441 ccagattgaa cggcacctgc acgaaccgga aatgatgcct gcccccggca atttcctgcg
     1501 cgatctgcga gatttcgccc agctgcatgg cgtcgcgcgc gcgggcatcc tgccggaatc
     1561 cattccacgt ggccatcccg taatactgaa tctcgcccgc ctccaccgcc gattcgagaa
     1621 acgtgaatgc ttcgcggaca cggctcagaa actcttcctt cgtcacttca gagagctgcg
     1681 tctccggatt gtgcaagtag tagacgtcca cgcactcgac gcccagattc ttcaggctgc
     1741 gcccgatctg atttttcaga aatttcggtg tcatacaatg actgcccgcc gcgatatctt
     1801 tcgcggtgaa aactcccggc tgaatgtact cgcggaaaaa atattcgttc gggtcgccgg
     1861 gcatcgctcc atcgggcgtc aggtaaccgc ccttcgtgca caccacgatc tcctcgcgtt
     1921 cgaatccttt cgccgcaagc tgccgtatgg ctgcgccgat gctcctctcg ctccgctgaa
     1981 atctgtaatt gatcgcggcg tccatcacat tgatgccgct ttccaccgcc gcgacggccg
     2041 ccgctgcgta gccctcatcc gtctttgcat ctggctgccc tagatacgtg ccgatcccga
     2101 gcgaggagag caccagccgc tgcgtttccc gaaagtggcc tgcggcggcg cggcccgcaa
     2161 acttctgcgc gtagcgggtc gttccttctt tggttgccga tttgctcatc atgctccttc
     2221 gggcggttgc gccggtccgg gtgtcggatt cgtttccact tcgttcgtct tcggggcgag
     2281 aagccgcgag cacgcccgaa gaatcctacg atagggccag tccttttcgc ggaagaaaat
     2341 ttcgccctcg cgtggattgc tgtaatacca ccgcgccacc agccatagat gctcgcccag
     2401 atccccgttg gttgttgtgg gctccgcttc aaaatgctcg cgaaagattt gctccgccga
     2461 ccgcggctgg ctggcaatct ccgcgaatcg caggaagaac ggctctgcca gccttccgcc
     2521 gcgcacgccg tagacgccaa tcgtttgctc ttccgcggcc cgctgcagaa tcacggcgtc
     2581 caaatcctgg atgcgcctgg tgagttccgg cctcccgcgc aaaacctcat cgagcttttc
     2641 cacttttttg tgcagcgcgg cggcgcgctc gaagtccaat tgctcgctgg ctttctcgcg
     2701 ctcttcttgg atgattgcgc gcagcgatcc gccacttgtt tccaggaatt gcaccagccg
     2761 ttgcacctcc acgtcgtatt cttctttcgt gcagcccgca aagcagggcg caaggcacat
     2821 tttcatttcg gagtacagac agcccgggaa cgtgggatcg cggcggatct tgatctggca
     2881 gcgccgcact ttgaagaggt cgaggatccg ttcggcgaat gcctgcgcga accgcctcga
     2941 tgggaatgga ccgtaatacg cgccgccagt gggcacgccg gcctcatcga cgggaatccg
     3001 gcgggtcacg tagcagcgtg gataagcatt ccgcaaattc aatttgagga ccgctgccgg
     3061 ccgcaggcgc atcaggtcgc gatagcgttc gggaaacagc agctttgcat gctggtagta
     3121 ggtgagtgtc tgttcgaacg gcgagccgat caagcgatag cggatgccgc gcgcaaaatc
     3181 gctcagattg agccgcttgc ttgccggatc gatcggtccg agcagccgtt gcagccttcg
     3241 ctgtagattc tgcgtgcgaa tcaggaacgg ctcggcattt tcttcgcgtg cctcgatcag
     3301 gcaaaccgcc gcccgcggcg gcaaggcagg gaagaagtgt tcttccgccc ccgccgcgtc
     3361 aaattcgagt ccacaatcca gttccagcac acggtcagga tactacgggg agctaagact
     3421 tcagaactca gcgatgggat aaaggaagga aagaaatctt tcaaagaaaa agaaacagga
     3481 tgctaagact tcagaaccca ggaagagatg accaggcgct ggatctcact ggtaccttcg
     3541 tagacttctg taatgcgcgc gtcgcggtag ttgcgctcga cgggatactc gcggctgtag
     3601 ccgttgccgc cgtgaatctg aatagccttg tgtgtgactc gcgtggccat ttcgctcgcg
     3661 aataatttcg cgatggctgc atccattgtg aagcgcgctc ccgtgtcctg cttccatgcc
     3721 gccttgcgga tcaacaaccg tgccgcgtca atttccgtgg acatgtccgc cagcatgaat
     3781 tgaatggctt gaaattgcga aatcgggtgg ccaaaagcca tgcgctcctg agaatacttc
     3841 agcgccgctt cgaacgcgcc ttgtgcaatg cccgtggcct gcgcggcgat tccgatgcgc
     3901 ccaccgtcca gcgtcgagag cgccactttg taaccctcgc cttcgttgcc gatgcgattt
     3961 gccaccggca cttcgcaatc ggagaaaatc agctcgctgc aggcggtggc actgatgcct
     4021 agtttttttt cttccttgcc gaccttgaat cccggcgtgc ctctttccac gaccagcgcg
     4081 gtgattcctt tttcgccctt ggcgggctcc gtgttcgcgt agacgattgc cgcgtccgcc
     4141 gcgccgccat tcgtgatcca ggcttttgtg ccgttgatca cataggtgtc gcccttcttg
     4201 acggccttcg tctgaagcgc ggcggcgttc gaaccggctt gcggttcagt caacgcgtag
     4261 catccgatct tttcaccccg cgcgtagggt agtaggaatt ttttcttctg ctcttccgtt
     4321 ccgtagcgat ggattggatc gcagtagagc gagttctgca ccgagagaat cacgcccgtc
     4381 gaggcgcagc acctcgaaat ctcttcaatc acgatgctgt aagcgatgtg gtcgaagcct
     4441 gcgccgccct ccgattccgg aaacgacact cctgtgagcc ccagttccgc tgctttcttg
     4501 aaaagcgcgc gcggaaaatg tcccgtctcg tcgagttctt tcgccagggg ccgaacttcc
     4561 gattccgcga actcgcgcac gctcttctgc agcagttgtt gttcttccgt cagttccagg
     4621 tccaccgtcg ctcactcccg tccggccaaa tactttttcg aattgcgtat tccatcgcgc
     4681 tccaatagtc ctgcgaatta cccccctcgc caggatgggc ctttgtaggg tccgccttcc
     4741 gaggcggacc gctcgcttca gccgagcgcc ttggagagac gctcgtaggt ggtcttcaaa
     4801 tcttccgggt gcacgcgcgt ctcgcaagtc accgtcatga agtttgaatc cccgatccag
     4861 cgcggcagcg cgtgcaggtg taaatgtcca accacgccgg cacccgcggc gcggcccaga
     4921 ttcattccca ggttcatccc atcgggtttg tagttcgcgc tcaaagccgt ttccacgcgt
     4981 tgcgccagct gcataatctc gccgagcgtt ccggcatcac acaaattcaa ttccgctaca
     5041 tgcgcgtacg gcacgatcat cacgtgcccc gaggtatagg gatagcgatt caggatgaca
     5101 aacgtcttct cgccgcgatg gacgatgagt gtttccgcgt cattctttcg cgacaccgcg
     5161 tcgcaaaaaa tacattccgg ctgctttcca cccgacacct gcgccatgta ctggtagcgc
     5221 cagggagtcc agatatagtc catcgcatcg cttccttttt ctcaagagcc gcgcgcacag
     5281 cataacgcag aattggcaat cgagtaccac gcgagcgctg attcaacctt agcgtgctcg
     5341 aaggcctgct ttatgttttc tgatgtgaat ggaaactgct cgaacaggaa ttctcttgcc
     5401 agccggattc cgagtctatt cgccgcctgc cggcgccgcc tgggcttctg aactattcac
     5461 aaagtccttc agctctttgc tgggcttgaa gaagggaatt ttcttggctg gcacttccac
     5521 cttcgcgccg gttttcggat tgcggccgac tcgtccgcgg cgctcccgcg tgcggaagct
     5581 gccaaatccg cggatttcga tcttttggcc cttctgcagc gatccaatga tgctatcaaa
     5641 aatagtttcg acgatggtct ccgactcctt ccggggaagc tctgtgatgc gcagaacctc
     5701 ttcgatgagg tcggccttcg tgagtgtcgg ttgcttggtc atcgtcgcta tgcctcgtcg
     5761 cacaactcta gattaaaaac cgtctcagtt cgtccaccgt cttacggtcg ccaggagcca
     5821 tgaccgtctc acggtcgcct ggatcaccga gcgctcagcc gtcgccagga acacctgccg
     5881 ctcagcgctt gccctaaaca gtggaaccaa acggcttaca gggggctggc ccctcgccgg
     5941 agtgccccct tcctatcatt aaaatgaccc gcaggtaaat gaaattataa cggccggccc
     6001 ccggcggacc ggtcttgaat cagctaacgc gccgacagct tggacttcag cgcgtccccg
     6061 atcgtcgccg tcgacgaggt tttcgacgtc gagcgatact gttcgatctc cttgcgctcg
     6121 atctgcttca ctgccgcgcg gtagctcaga ccgatccgcc gcgtctccgg gctgatcttg
     6181 acgatcttga aatcatattc tttgcccggc tcgagcggcc ccgcactctt cagcggcccg
     6241 gttgtcggac ggaaactcga gggtccatcg tcacggcgct tgcgctcctc aatttccgtg
     6301 ttatggcaga gcgcttcgat gttttcgccc aattcgacga acgcgccgaa cgtggcgatg
     6361 cgcgaaacct tgcccttcac gatttgcccg accttgtgct gtttgaacca atcgccccag
     6421 atgtcgttga cctgtttgat gcccagcgac acgcggcggt tctccgggtc gatcttcagc
     6481 accttggcgg tgatgtcatc gcccttcttg aacacctcgt gcggattctt aatgcgctcc
     6541 gtccagctca catcgctgac gtgcaccagt ccatcgaatc catcttcaat ctcgatgaac
     6601 gcgccgaact ccgcgatgtt ccgtactttg cccgtgatga ccatccccac cggatatttt
     6661 tccgcaatca ccagccaggg atcgggtagt gcctgtttga ttcccagtga aacgcgccgg
     6721 tcgctcggct tgatgtccag caccaccaca tccacttctt caccaacttt ggcgaccttc
     6781 gacgggtgct ggatcttctt gttccagctc atctcggaca cgtgtaccag gccttcaatc
     6841 ccctgttcca actccacgaa cacgccatag tccacgaccc cgacgatctt gcctttcacc
     6901 ttcccgcccg ccggatattt ttccgccgct cccacccagg gatcagccat cagttgcttc
     6961 atccccagcg atacccgctg cttttccttg tcaaacttca ggatgatgac gtcgacttcc
     7021 tgctccggct ttaccacgtc ggaaggatgc ttcacgcggc cccagctcaa atccgtcagg
     7081 tgcagcaagc cgtcgattcc gccgagatcc acgaacgcgc cgtactccgt cacgtttttc
     7141 acgtgcccgt gcacgacctg gccttcgcta agcgtttcca tcaacgtggc gcgcttggca
     7201 tgcagttctt cttccagcag cgcccggcgg ctcaccacca cgttcccgcg gcggcggttc
     7261 agtttcgtga tgcgcacctg aatttctgtg ccggccaggt tgtccaggtt ctgtgccggc
     7321 cgcaagtcgt actgcgaacc cggcaggaac gcgcgcacgc caatatccac tacgaggccg
     7381 cccttgatgc ggtccactac cttgccggta atcgtttcct tggttttgaa cgccgcttcg
     7441 atcttctccc acgttttgcg ccgcagcact ttcaggtacg acaggattgt gaagccgtcc
     7501 ttgtgctccc cggtgcgctg cacctcgatc gtactgttcg gctctggccg cggtatctcc
     7561 gtgtccgtga attccgccgc cggaatcagc ccttccgtct ttccgcccag gtccaccacc
     7621 acgccatgtt ccgtgtacgc gacgaccttg acctcgatca cctcgtgcga cgcagccgct
     7681 tcctgcttct cgtcgtactc ttccagcagc ttgctcatct cttccgagcc ggcagcttcc
     7741 tcggccgcag ccgcggcctc ggggtccgcc gcgtgcgttt cggcaacagg cttcgcctgt
     7801 tccgctgccg ccaccgcggg cgtctcttgc gaagtgttca cagcttcggt gtgctcggtc
     7861 gcagcgggag gagcagtttg ctctgtcgcg ctttctaccg ttgaatttgt ttcggtgtgc
     7921 gcgctgttcg gagcggtttc gccttcgggt gcagccgcgg cagccgccgc attggactgg
     7981 ttatcgtggt cggaaaacat ggttgttggc ctccggaaga ttacttcgct ttgggtagcc
     8041 gctaagcggc cccgcgttca gtcgaaaata gtccttgctg cttcccaatc ggggttaatg
     8101 caaggaagtc aaccgccctg cgcttgcccg gcaatatgac cgcttttggg gggcctattg
     8161 ccaagtcagc gggtcggcgg ccgcttagag acaaagatgc tgcggtcctc gaaaacgctg
     8221 caaccttaaa aggatactcg ccgcctcgac cccctgtcaa tgaaccggcg ggacgagctc
     8281 taggcgcgtc cggcgtgcgt cttttcgcgc cgaaaatccg gagcactaac ttccacggtg
     8341 cggcacatct catacaacct agaacgcatt cgactcccga ttcgttgctc cagggcatct
     8401 tcgcgcgtcg gtgcaatcaa cttgccgttc ggtagccgcg ccgcttcgcc ctccgtagcg
     8461 gggttatcga gatagttcgt cgtgatgatc gtcgtccgat tttcgttgta tcgggcgttc
     8521 aagacaatcc caacgatgtc tcgcacccag tccgacggct tgctcgcgcc gagatcatcc
     8581 agcaccagaa tctccaccgt gcgaatcggc tcgagaatct tcatctctgt ggattcgctc
     8641 gccgggttgt agctcgcctg aatttccttc agtagctcgc gataatcgca aaacaatccc
     8701 gcatgcccgc gatgaatcag ttctttcaat gccgcaaccg ctagatgtgt ttttccaact
     8761 cccgaaggcc ccatgaacag caatcccttt tcggaggaac cgggatactc gcgcacaaac
     8821 ccttgcgtga acagttttgc ctgttttagt gattgcgcgt gctgcgccgt ccaggtcttc
     8881 ccgtcggtca ggtcggtaac gtaactttca aaatcgcaat gttcgtagcg cttcggaatt
     8941 cgcgcgcgct ccatcacccg cgaagcgcgc tcctccatcc cgcaatcgca cgccaccgcc
     9001 acgctgcccg tcgcgccatc cccgcgcggc accagcttcc atcccgttcc cctgcaaagc
     9061 gtgcaatttt ccagcgccat atgtattcgt ctatgtaggg ccgggtttca gcccggccct
     9121 actctttcct tggcctctgt agtggctagt cttcctcgtt cttggcctca cggccttgcc
     9181 gggaagaccg gttcatttcc ccgcgccaga cagcatacga gcgctaaccg cacacggcaa
     9241 gctgtctgca ttgggaaaac tgtggaagga attactctat cttttctctg attactgata
     9301 actgatagct accgcacaag ccgcggcgtc gcccgcacga tcgcatgccc cggcgttttc
     9361 tcctgcatgt gcgacgccgc cacccgcgac aattcttgct gtaccacatg cgcgaactcc
     9421 cccatctgcc gctccatctc gatcatcttc tcgcgcatgt tcagaataat ttcgatgccc
     9481 gccaggttca cgcccatatc ccgcgccagc gtcaggatta cgtccagccg ctccagatcc
     9541 gaatccgtat acaaccgcgt gtttccctgc gaacgcgatg gcttcagcag ccccacgcgc
     9601 tcgtaaagcc tcagtgtctg cgggtgcagc ttgtacagct ccgccaccgc gctgatcatg
     9661 tagcccgcct tggcccgttt ttttgctcgt ggcggcatcg tcgatttctt acactcctgc
     9721 cttgctgaac aaatcctttc gcggatcccc cggcgcgacg ctctccaatt ccttcatcaa
     9781 atttcgcacg cgttcatctg tcggcggagg cacgaccacc tgaatctcca cgtactggtc
     9841 gcctctcgcg ccattccttg cgctcggcac gcctttttcc ttcagccgca gcgtcttccc
     9901 gctgttcgtc cccggtggaa tcctcaccag cgaccgcccg tccatcgtcg gcacttcaat
     9961 tttcgcgccc agcgtcgcct cgctcaccgt aaccggaatc ttggtataca aatcgtcgcc
    10021 gcgccgttcg aaaaactcat gcggcttcac caccacacgc aaatacaaat cccccgccgg
    10081 cgcgcccatc gttcccgagt tgcctttgcc cggaacccgc acccgcccgc cattcgaaac
    10141 gcctgccggg gtgcgcacct caatcgactc cgtccgccgc acgcgccctt cgccgctgca
    10201 ggtcttgcac agtgttcgca gcttgcccgt cccgccgcac cgtgtgcacg gcacgttgaa
    10261 gcgcatcttg cccgcggcct gctggatcgt gcccgttccc gcgcatgccg tgcagatctg
    10321 cggcgagccc accgcgcctg ttccgtggca cgtctcgcaa gcgtccattc tcgtaatcga
    10381 aagcttcttt accgcgccgc gcacggcatc ccaaaaatca atctcgatct ggtattccag
    10441 gtcgccgccg ggttcgtgct ccggctccat ccccgcgctt ccgcgtccgc cgctgaaaaa
    10501 ctggctgaag agatcgcgga agctcgatcc gccgccggcc gcgcccgagc cgccgccaaa
    10561 atcaaatccg ccaaaatcaa aattgacgtc cggctccccg ccgtggccgc ccgctccacc
    10621 tcgcccgccc ggtggaaggt tgtcgctgta gaagccgtac tggtcgtaca tctgcctctt
    10681 cttggaatcg gagaggacgt cgtaggcttc ctgcagttgc ttgaattttt cttccgcgga
    10741 tttgtccccc ggatttaaat ccgggtgata cttccgcgcc agcttgcgaa atgccgcgcg
    10801 gatatcctta gcgcccgcct tccgcgctac tcccagcagt tcgtaataat cctgtttcgc
    10861 tgtcgtcggc atcgaacact atgtgcgtac gggcttctgc ggccgtccgc actcccacgc
    10921 ttcctctcgc ctgtagggac ggccttctga ggccgtccgc tctgcgcttt ctcagttcgg
    10981 ccgcttgctc tcgtccacat ccacgtactc agcgtcaatc acatcgccag gcttcttctc
    11041 ctgcgcgccg ctcgggccac cagcagaact cccgcccggc ccagcgcctg cgctggctgc
    11101 cccagcgtgc tcaggaccac ccgccgcctg cgtcgtcttg tacagttcct cagcgatctt
    11161 gtgcagcgaa cgctcgacgt tttccgttgc cgaccgcagc cgcgccacgc cgccttcgtt
    11221 gatggctttc ttcgcgtctt cgatggcctc ttcggccttc ttcacgtccg cctccgcgat
    11281 cttctcgcgg ttttcgcgaa gcatcttctc cgattggtag aggatgccgt ccaagcggtt
    11341 gcgtgcctcg acttcctcca tccggcgccg gtcatcttcc gagtgcgatt cggcctcggt
    11401 ccgcatcttc tcggcctctt ccttggtcaa gccgctcgac gcggtgatgg tgatcttctg
    11461 ctctttgctc gtggccttgt cctttgcgct tacgttcaga atgccgttcg catcgatgtc
    11521 aaacgtcact tcgatctgcg gcgtcccgcg cggagccggc ggaatcccgt ccagcttgaa
    11581 tttccccagc gaacggttgt cggtggcgaa cttgcgttcc ccctgcatga cgtggatttc
    11641 cacgcccggc tggttgtccg ccgccgtcga gtacgtttcc actttgcgtg tcgggatcgt
    11701 cgtgttgcgc gggatttgca ccgtcatcac gccgccgagc gtctccacgc ccagcgaaag
    11761 cggcgtcacg tccagcagca ggatgtcctt tacttcgccc gcaagcactg cgccttgaat
    11821 cgccgcgccc accgccacca cctcgtccgg gttcacgccc ttgtgcggtt ctttcccgcc
    11881 gaaaaaagtc cgcaccagcg actgcacttt cgggatgcgc gtcgagccgc ccaccagcac
    11941 cacttcctgg atgtcgttcg gcgacatctt cgcgtcttcc agcgcctgct tcaccggctt
    12001 catcgagcgc tccagcaggt cattcatcat ctgctcgagc cgcgtgcgcg tgagcttctc
    12061 ttccatgtga atcggcccgt tcgcgcccgc ggtgatgaac ggcaggttga tctccgtctc
    12121 catcatctgc gaaagctcga tcttggcttt ttccgccgca tccttcagcc gctgcagcgc
    12181 catcttgtcc ttgctcatgt cgatgccgtt ctttttcttg aactcatcga tcagccagtc
    12241 cacgattttc tggtcgacgt cgtcgccacc gaggtgcgta tcaccgttgg tcgatttgac
    12301 gtgaaccacc atgtctccaa cttcaagaac cgaaatatca aacgtgccgc cgccaagatc
    12361 gtagacagcg atcgtttctt catttttctt gtccagcccg tatgccagcg ccgccgccgt
    12421 cggctcattg attatgcgca ctacctccag gcctgcgatt tcgcccgcct gcttcgtggc
    12481 ttgccgttga gcgtcgttga agtaagccgg cacggtgatc acggccttcg tgactttctc
    12541 gcccagaaaa tcttccgcgg cgctctttag ttttcccagg atcatcgctg aaatttgcgg
    12601 cgggctgtag accttgtcga aaatctctac gcgcgcgtcg tcgtggtctc ccttgacgac
    12661 cttatagggg atgcgccgca tctcttcgcc cacttcatca aaccgccggc ccatgaaccg
    12721 cttgatcgaa tacaccgtgt tctcggggtt cgtcaccgct tgccgcttcg ccacttgccc
    12781 gaccagccgc tcgcccgttt tcgtgatggc cactaccgac ggcgtcgtcc gcgctccctc
    12841 ctggttgggg atcactaccg gctcgccgcc ctgcatcacc gccactaccg agtttgtcgt
    12901 tcccaggtca attccgataa tcttgctcat tgctgccatt cctcctgaag tctcttgcaa
    12961 ttccgttcgc ccgtatctct ctcaaatcaa ggcgaatcca gctcagttct cgcgttccaa
    13021 gcccagctcg aagcgcagta tacatgttga gtgtatgttt gtcaagtttt cttacaacaa
    13081 atataactcg ttcattttct tagtattgca gccgccggtc tcttctccgc gctccgctcc
    13141 gaaaactcct cttgactctt gataatgtat cgtgtacaat acttcttcga tactggtggg
    13201 cggaccagtt gggagggtta ctcatgtcct ttcgccaacg cccattcccc ctcgcaattc
    13261 gtcataaccc ttttagaatc cgcagttcca tgacccctct cccgcaaccc ctttataatc
    13321 cgcacttaca gacacccctc gtaagtgcgg gaaacaaagg agttataact cctttagaat
    13381 ccgcacttac agaaaatccg cctgtaagtt ctttagaatc cgcacttcca aaaagtaggg
    13441 ggagggggta ttttggttag ctagaagcct cggcgcacaa cgcttccggt ttcgtccgca
    13501 accaactttg aaggacttcg gtggcggaac atctcttggt gcttatatga acctcggacg
    13561 acgacaactc ttacattccc tcgatttcgc ggtagaattc tgtttcgtca cccgcaaggg
    13621 cgcatcgctc gaaccatttc ggaggatcgc gtgaaccgtc ggcgcattct ggcagggatt
    13681 ctcagcgtgg gtttgcttgg cttaattgcc ttgttcctac cttctttgca caaatctcct
    13741 gagcccgctt caccatcgtc acaaccgttg gcgcagtcgc cgtcagagtc tctaggctca
    13801 aatgcagaag ctgagcccac ggttattccc acgtctgtgg tcgcggcgaa agctgcttcg
    13861 cgtccgccgg cgccgcagtc agcggctggt ctccccgacg tggatccgca caaggccttc
    13921 ggctcgaaaa acgctcccgt gaccatggag gtcttcagcg attttcaatg cccagcttgc
    13981 aagaccctct tcaccaccac caatcgccgg ctcatggatg attacgtcag cagcgggaag
    14041 gtctacctga tccaccgcga tttcccgctc cccatgcacg cgcactcgcg ggtagccgcg
    14101 cgctacgctc gtgccgccgc gcagctcggc aagggtgagc ctgtggaaca agctttgttc
    14161 cagaatcagg aaaagtggga acagaacggc gacgtggacg gcaccgtcgc tgccattctc
    14221 tccgccgcgg aaatggccaa ggttcgcgcg ctcgtgaagg gcggcaccct cgatcccatg
    14281 attgacaaag actacgcgct tggccagacc taccgcgtca accagacgcc caccaccgtc
    14341 tttcattgca aaggccagac ctatccctac tccggcgtta tgacctacga cattctcaag
    14401 acctttctcg agcaactgct gtcacaaaaa taaatgatgc aatccagccc actcaatcta
    14461 cgccgcgcga tcatctggat cggccgcctc gttctcgccg gcatcttcat ctatgccggt
    14521 tacgcgaagc tgactatggg catgcatccc cgcccgccga tccgtgttgc cctttcgttt
    14581 ttcgcgctgc aagtggattc ttatcaaatt ctcccatccg ggggcgtcaa gtttgtcgcg
    14641 aacactctgc ccttcgcgga aatcggcctt gggcttctgc tgctcctcgg ctggaggttc
    14701 cgtatctggg ccacgctcgt caccttgatc atgctcggat tcttcgccgc cgtggtgcgc
    14761 tcttacgctc tcggcttgca gatcaattgt ggctgcttcg ccactcccga accgctcacg
    14821 atcaaaacgg ttttccgcga cggcgcgctc gtcgctctcg cgctcctcat gaccatcttc
    14881 gccttcatgg aagcgcgcaa gccgcatccc tggtccgccc cggagaaagc ttgagctgca
    14941 cttgctccga tgaaaactct cgctgaatac ctcgatctcg ccgctgtcgc gcacggccac
    15001 ctctgcgccg gacaggtcct cggcgtgcgc ctcgccatgc tcggcctgcg tgaactcggc
    15061 atcgtcgatc ctgtcgccga gcgcaaacgc atcgtcacgt acgtcgaaat cgaccgctgc
    15121 gtcaccgacg ccgtcgcgct tgtcgccaat tgccgcctgg gcaagcgcgc cctgaaattc
    15181 cgcgactggg gcaaagtcgc ggccactttc gtcgacctcc agaccggccg cgccgtccgc
    15241 atcgccgcca aagaatcgtc caagcaagcc gcccgcgaaa tgttccccga actcggcaag
    15301 gacgccggcc agcaaaaagc ctacgcagaa ctctccgacg aaatcctctt cgacaaacaa
    15361 tgggtcaaag tcgaagtcca gcccgaagat ctgcccggct tcaaaggccc acgcgttgtc
    15421 tgcgcccaat gcggcgaagg catcaatttc aaacgtgaag tcgtgaagga cggcagcacc
    15481 ctctgccgcg cctgctccgg agaaaaatat tacgaaacac tgtcctgata cttctcagtc
    15541 gagtattcga cttctgcgat aaagtattct ttcgatgctg agcttgccac caattacgct
    15601 gcgactgcgc ggctggaaac tgattgaccg cgtcgcgctg gcttttctga ttctttgcgc
    15661 tgttgtgctg ggcgcgggct gcggcttgct ctttgtttac gccagcgacc tgccggaaat
    15721 tcgcgcgctg gaaacctacc gccccgacgt ggtcactgaa gtttacgcgg atgacggcca
    15781 gctcgccggc agcttcgcgc tgcagcgccg catcctgatg acgtacgagc agtgccccaa
    15841 agtgctgtac agcgcggtca ccgccatcga agaccagcac ttcgaagagc actggggcat
    15901 cgacttcccg cgcatggcca gcgccgcggt gcgaaatctc atcaagcgca aaatcactgg
    15961 cggcgccagc accatcacca tgcagcttgc cggaaatctt ttcctggacc gcagcgaccg
    16021 cagcttccgc cgcaaaatgc aggaaattct gctcgcgctg cagatcgagc gccgctacac
    16081 caagccgcaa atctttacga tgtacgccaa ccaagtgtac ctcgcgcatg gcaactatgg
    16141 tttcgccgcc gctgcgcaat tttattttgg caagccggtt accgacctga aacttcagga
    16201 agccgctctt cttgccggca tggttaacgg cccgaagttt tcgccgctca caaatccgga
    16261 actcgcgctc aaccgccgca atctggtgct ccatcgcatg gaagaagaag ggaagatcac
    16321 gcccacggaa gagaccacca ataggaaatc gccgctcggc ctgcacattc agtatccgcg
    16381 caacgacctc gcgccttact tcttcgaaga gattcgcaag tatcttgaat ccacgtacgg
    16441 cagcgaagcc gtccacgagc gcgggctgcg cgtctacacc acgttgaaca tcaagatgca
    16501 gcgcatcgcc aaccagtctc tccgcgacgg tctgcacaac tacgaacgcc gccacggctg
    16561 gaagggcaat cttcccaacg tcctgcgcga caatcttggc aaactcgata cctacgaaga
    16621 cgacgattgg cgccatgcga tcgagaaggg aagttacgtc accggactcg tcctcacggt
    16681 gagcgacaga gacgcggtca tcaagatcgg gacttaccgc gggattcttt ccgccagcga
    16741 gttcggctgg accggccgca agaaaccctc cgatctcctg aaagtgggtg acctcgccca
    16801 attctccatt caggaattgc gtgacaatac cgtccgcgtt cagctcgaac aacagcccgg
    16861 gccgcagggc gcgcttctgg ccatcgacaa ttccacgggt gaaatcaaaa ccatgatcgg
    16921 cggttatagt ttcgaggatt ccaaatttaa ccgcgcgacg caggccgtcc gacaggtcgg
    16981 cagctctttc aagatttatg tctacgccga cgcgctggaa agaggcgcga cgccctttga
    17041 caccatcgtc gatttgccgt ttaccaccat cagcggcggg cagccctatt ctccgcgcaa
    17101 ttacgatgaa aaatttgaag gcaccatcac gctccgccgc gccctggccg gttcacgcaa
    17161 cgttcccgcc gtaaaactcg cggaaaaaat cggcatcaac acggtggtgg acatgacccg
    17221 gcgtttcggc atcaccacgc cgcttccgcc gtatctgccg ctggccctcg gcgccgcgga
    17281 catgaagctg ctcgaacacg tctcggcctt caccgttttc cctaatgatg gcatccgcat
    17341 cgatccgcac atgattcgcc gggtcaccag ttacgacggc gcgctgctcg aagaagcgca
    17401 tcccgaggtg cacgacgtcc tctcccccga cgtcgcgcgc accatgaccg ccatgctcga
    17461 ggaagtcatt cggttcggca ccggcatcca ggccaaagca ttgggccgcc ccgccgcagg
    17521 gaaaacggga acgactcagg actacaccga tgcctggttt gtaggctata caccgcagct
    17581 caccaccggc gtgtgggtgg gcttcgacga caagcagatt tcactcggga agaaagaaac
    17641 cggcgcacgc gccgctcttc ccatctggct cgaattcatg caaggcgcgg tggccggaac
    17701 gcccgcgatg gatttcccga atgtcgttcc gctcgagcag caggctggcg agcatcacgt
    17761 cagcgtggac accccggata ccgctcccac cgaagacgaa cccgccgccc ccaaagaaaa
    17821 accgccggcc gttctaccgc ccaccagagc gggagctgtc ggggcaaccg gcgtatcgca
    17881 acgccgctga atgcggggct gccacatgtt tctgatcccc ctagttcacc gctacagttt
    17941 ttgaaccaca acgtttagag aagaagtctg gatcgcaccc aggtttacct gagcggggtt
    18001 catccccagg cttaactgcc acagctggtg cccggtactg tccgtcaagt tcagattcac
    18061 cgtgaattgt gcgctgatgc cgagcgcgct tgggttggag aacaagacgc actgcgcttg
    18121 cccatttatc agaggtgcgc tcccctggaa gctgagcagc gaggaagaga tcgaataatt
    18181 aatcgttcca gccacgggcg tgccgtcggg atacttgaaa ttcagcacca aaaccggtga
    18241 tgttgccgga agcgggagaa tttcgatggc gctgatcttg ggattcagac ccgatacgtg
    18301 ggcgaatgca atgttgattg caccggcggt cacgttgacg ttcgccgatt tgattaatgc
    18361 ggcgttggca ccagcggctg catagatgtc caaatttgcg aaaaccatct ccccttggag
    18421 actaacattg aatacacggg ctcccacgga ctcggcctta ggagcggctt cggtgaagta
    18481 cagattgact tggtacgccc cattggaaac cgggaaggaa taactcgtgg gattccaccg
    18541 gtaatcctcg tacaggagcg gatccgaggt gccggcgact gagccactaa ttgtctcttc
    18601 tgagccgcca gtaaagccgc tgtctgcttg ccaaacttga cctttcgaat cggtgtaact
    18661 cggtccgcca caattcacgc ggatgagttg ttgcgccagt acccctttcg gaaaacaaag
    18721 cgccgtcaca aatgccaaca cacaaaaaag ctttcgcacg aagcctccag ggaatcggtt
    18781 ttcagattct agttttctaa gtcaagcgag gagggctaag ggaagggggc ctgctcgaac
    18841 tttccatagc caaagtacag gctcagcggc atacaaacaa ttggtcatga gaactgattc
    18901 aggccaggag tggaaatggt ccgaatgctg tacggaggag tgtaccaatt tgaacagccg
    18961 caattcctgg caggttcgaa tcttgcccgt gtctacagct ttttccggaa gggatcccag
    19021 ccgctgggca agagttgcct tgcgtggcca ctcgcttcca gcaccagcaa cgctcgatct
    19081 cgggtgattc ttccaatcgc agagagaccc acacctcgga atgtcttcgg caggagcttt
    19141 gctttgcgcg gaggcacggt gaacaacaat tcgtagtcgt cgccaccatg caacgcgagt
    19201 tgcatcggat cacggccatg aatgagagca acatcgggga tttgcacctg cggaatgttt
    19261 gcccagtcca atagcgcacc gaccgcgctc gccgcgcaga gccgcggcag atcgctcgat
    19321 agtccatcgg agaggtccat catcgccgtc gccaagccct tcttgaccaa ccattggcct
    19381 agcgcgagac gcggctccgg ataaagatgt tttcttgtca gctggttctt cctgctcgcc
    19441 gctcctttgc ttcgtcgcac gatccgtaga cccaattccg cctcgcccag ccgtccgctc
    19501 acgtaaagaa tgtcgcctgc tcgggcaccc gaccgcaacc cggcgcgccc ggcaggcact
    19561 tcccccacga cggtcacatt gataagaatc tcgtttcgcc gcgtggtgtc tccgcctgcc
    19621 agcgcacact gaaacttccg agatgcacgc tgcaaccccc cgaggaacag atccagccac
    19681 cggccggtgt gcgtctctgg cagggccagg ctcagcagga agcagcgggg cgtgcccccc
    19741 attgccgcta tatcgcttac cccccgggcg aggcacttcc aaccgaccgc atccggcgga
    19801 tgcttctccc gcaagaagtg ggtgccttcc aggaaccagt cgcaggtgag aatcgtttcg
    19861 tgcccgggct ttggccggaa gagcgccgcg tcatcgccaa ttccaagagc gacggaatgc
    19921 ccggctcgtg ggacctggga cgatcgggaa ctgtctgcaa tcttcctcag gatagaatct
    19981 tcgctctggc gtttccgcgg catggggttc tgagaggact ccaatctacc tgcgggcgac
    20041 agtatagctc gtcatgctac cctgtctctc gcggtcagaa ctggggcatt ggggtaggca
    20101 atatatggct cctgtactct agggcagggg acaggtttgc acagcctgac cttcaaagca
    20161 cgaaatacag ggttgacgtc caaccctaca gaatttagta tgtccaagtt cgcggaccaa
    20221 ggctcacagt tcagattttg gttcaatttc gggttccagc gggtaccagg gtaattcgcc
    20281 cgcaaacgtc aaattgaaag gcctgagggg tcccggtaat catgcggggt aaaagctata
    20341 cattttatat cgcgtcgtcg ccgggtaagc tcaaaaagct tatcgtaccg gcttatgtgc
    20401 tgcatggcat cgcggtattg gcgctcatcg gcggtattac ggtgacggcg gcggtgggct
    20461 cttattcgcg gatgctctgg aaagtcggca actacaacgc tcttcgccac gatcaggaaa
    20521 ccctcaagaa gcagtatcag caactccaga gcaccgtcaa caacaccaac cagcgcctcg
    20581 attctctaca gtccctggcg acggaagtgg ccatgaccta cggggtgctc cgctatcatc
    20641 cggcggcttt cgatcagagc gacaaccccg tggatgccca ggatgctttt gaccgctcca
    20701 tcgagcagta cactttcctg aagcgcaatg ccgcggtgat tgccatctcc agtagtggac
    20761 tccatttgct ctcccccatg agttttgcgg actcgacgta cacgccggcg atctggccgg
    20821 tcatggggca cattacggat agcttcggtg agcgcttgga tccgttcagc ggcgaaggca
    20881 cgttccacac cggcgtggat gtagcttcgg actatggcgc tccggtgcac gcgacggcgg
    20941 atggaatcat caccatcgcg gagaaccatg cgggctacgg ccgcctggta gtcatcgatc
    21001 atggtttcgg gatcaccacc tggtacgcgc acctctccgc gttctcggcg gtttccggcg
    21061 cacgcgtgaa gcgcggcgaa gtggtgggct acacgggaat cagcggccgc tcaacgggtc
    21121 cgcacgtcca ctacgaagtt cggatgaaca acgcacccat caatccctgg cgctatatga
    21181 agtccactac ggcgggcgac taaatcttga ttgaaatttc gcgggtttcg tagcggcggg
    21241 gcttgccccg ccgctacttt tttgagggcg tcgtttcttg cggggaaagt cgcgcgcgga
    21301 tttcccccaa ctgtgttctt acgtgtggcc tttcctgggt gccggagctt ttcctgggag
    21361 cggtttaccc ggggcggagt ccggcatgac tggcggagct ttgcgtgccg cctccaaaaa
    21421 tcccggggtg atttcgaata cgagccggac ggatttggta tcccctcggt cgattttgga
    21481 gacatccgcg cttttcagaa gttccaggta agcctcgcgc atcgccggat ccaactgctg
    21541 cctggttctg gcatcattta gtcccccctg tgccagtatg acgacgccgt tcatcacgtc
    21601 cacgagttgc cgcaccgtcg cctccgccgt gcattctcct tcggcgacca cacggagccg
    21661 gtcgttttca ggtttacctg ccagggtgat ccactgcaat tgattgagca acgaggagag
    21721 ctgtggagag cggagtccgc cgggagcctg tgcggcgagg gcggcgccga ttgcggcgtc
    21781 ttgccggatc acggcaaata ccggcgagcc cgccagacgt tcgaatcgcg cgcgccattc
    21841 ggaaacgtcc tcggaccgtt ttttcgcgtc gaggaaaacg gcgaggtttg catcgttggt
    21901 aagcgcgatg cggtcattcc tcaaaaacgt aaaggaaatt ttcttagcgg ttccactgac
    21961 cggtacggaa aaaatctcgc gcccggccgt ttttacggcc gagccgtctc tcaaggcgta
    22021 agcggagatt ttttgccgat cgaattttcc atccaggaca gcaaaaagag gggagtcttg
    22081 tccgcgcctc tccaccgcaa tcgccacgcg atggaggtcc cgctcatagt caaatccggt
    22141 ttccttgaca aattgcgcgt aatccgcgtc cgcttgcggt ttgggcgccc acgcgtagag
    22201 ttgcgcgatg aagggcgatt gccggagttc gccgaaatcc acgaatagaa ttgcgctcgc
    22261 atccgaaggc atcagcgtca gcatctcccc gcgcgcagag ccgttctgta cactccattt
    22321 tcgatacccg aaaaatgcgg cggcgcttaa gaagacgaca actgccgcca gcccgatcca
    22381 tgtccgtttc gtcatggcct ccccaccatt tgcacctgtg gttctggagc ggcaattgtc
    22441 ccatcggaca gggcttcccg gcatcaaaat ccgatagcag aaatgctttg cgaacagcgc
    22501 tattcccggc tgttgagttc ggaccgctac ggctggctgg attcggtgct aaaccataga
    22561 ggatagacag gatagcgggt gtctaggtag tcaagggtgg gggcgttttt ccacagagct
    22621 ggccactgac gtgctagata tttggcggca accccttgca attctgatac cccttctgct
    22681 atccttgtgc ggtttgagcg gcgctggcgt agctcagctg gtagagcatc tgatttgtaa
    22741 tcagagggtc gggggttcaa ttccctccgc cagctccacc aggaattttg caggggagcg
    22801 agggcggagc gaatcctgac aggaaaaatg aacgggctgg tgtctcttga cacgagtcct
    22861 ttttgtgttt ttgcgcggaa cgctcgcaaa ttattgaagg aaaagttcgg gagtcgtgcg
    22921 tttgcgtccc aagaagtttt tgcgggatgc ggtttttgca tcccgaatga ttttacggga
    22981 cgggtgggtg agtggctaaa accagcagac tgtaaatctg ccgctccttg cgggctacga
    23041 aggttcgaat ccttccccgt ccaccaggct ctcgatgtgg ggttgcagaa aaccgcaggg
    23101 caatcgcatc aagcgatgtt cgaacgcggt ctgattcggg gcgccgaccg atttgcggtc
    23161 gcaacggaaa aaacgacgcc gctcaggaaa agtgcgccgc gaaggatgat ttttgagggg
    23221 g
//
LOCUS       DASYVO010000134         9419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_257913, whole genome shotgun sequence.
ACCESSION   DASYVO010000134 DASYVO010000000
VERSION     DASYVO010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9419
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257913"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VGR03_04450"
     CDS             <1..824
                     /locus_tag="VGR03_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEV1993560.1"
                     /translation="LPAVPIGEVMRAFGFAEIVESRHPDYKKGDRVTGLTGLQEYVIL
                     DEAAKRSFQKVPKIPFVSDTVFLGVLGINGLTAFFGMEIAAPKKGETLVVSAAAGATG
                     SIVGQIGKIHGCRVVGIAGTDDKCAWITKDLGFDAAINYKHPDWKEKLAAATPNGIDI
                     DFENVGGEIMQEVLNRMNLHGRVVVCGLISGYTKEDPGLASLGNILVKRLRVQGFIIL
                     DYASRFMEAATQLGQWKMFGKLKDRETIVEGLEKAPDAINMLFTGGNTGKLIVKV"
     gene            complement(848..1249)
                     /locus_tag="VGR03_04455"
     CDS             complement(848..1249)
                     /locus_tag="VGR03_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993561.1"
                     /translation="MSDMSVSRKAALWVGVVFLLGAALGGMIGYVFAHHTALAAPPQM
                     TEAAKRAQKVQRLTKELDLSSEQQKQLEVIIASVQAQYKAIHQSTDPQINEARQKGRE
                     QIRAILTPEQKPKFEEFLKRLDEDRKRNAHQ"
     gene            complement(1246..1761)
                     /locus_tag="VGR03_04460"
     CDS             complement(1246..1761)
                     /locus_tag="VGR03_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993562.1"
                     /translation="MWNLLKKSDEACSTLRDLLEDSAAARPEAIRVEQLSEAWPAAQR
                     AHFAACGNCREAAQDLLATREIFKGVASRAGQAGPWFAAGVMGAIRSRERELALPASL
                     WGAVPRHASRLAWVSAVVLLAGSTWLYERPAAAPNKQPAAATSQEYLFEAPQQPMNQD
                     DVLISMAEKNQ"
     gene            complement(1793..2443)
                     /locus_tag="VGR03_04465"
     CDS             complement(1793..2443)
                     /locus_tag="VGR03_04465"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1993563.1"
                     /translation="MAHVPKVEPFDPPPAVGAVQARASKPVGATDDRELVRRAQGEDQ
                     EAFEELVRRHQHRVFAVAGGILRRRDDVEDIAQQVFLKAYFSLKKFDQRAAFSTWLYK
                     ITVNECWDLLRKKKVRPLLYESDLSEEQARQVLTSGEKENPGPDISEKLEARERVERL
                     LEGLDERDRLMLILKEVEGFSIEEIAEVLNLNGNTVKVRLFRARRRVASQAKKRGD"
     gene            2560..2931
                     /locus_tag="VGR03_04470"
     CDS             2560..2931
                     /locus_tag="VGR03_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1993564.1"
                     /translation="MIIGDRLRALREEKKLSQGDIEKRTGLLRCYISRVENGHTVPAI
                     ETLEKLARALECPLYQLFYDGEEPPQLPNLPKRKSSDDIAWGSVGKDARFLNKLRRLL
                     SKVEEGDRKLILYMAQKMANR"
     gene            complement(3107..4738)
                     /gene="guaA"
                     /locus_tag="VGR03_04475"
     CDS             complement(3107..4738)
                     /gene="guaA"
                     /locus_tag="VGR03_04475"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV1993565.1"
                     /translation="MMESAASQVTEQAREQGGRMKAELGGVVVLDFGGQYTQLIARRI
                     REQQVFSAVLPCTTSIEEIRKLEPAGIILSGGPSSVYDADAPKCDPKVLALGIPVLGI
                     CYGMHWITHTLGGTVEKAERREYGRAQLSLGDAPKTGGSELFTGIPGSLRVWNSHGDH
                     VRALPEGFRTTARTENAIGAVENVEKKIYAVEFHPEVNHTEHGTELLRNFLFRVCDAE
                     PKWSGAAFIEETTAAVREKVGNKWAICGLSGGVDSTVAAVLVHRAMGTRLTNIFVNTG
                     MLRKNEFEQTLEMLRDRLKLHVIGVDASERFLAQLKGVTDPEEKRKRIGREFIAVFAE
                     EAQKLQAGEAHGEIGYLVQGTLYPDVIESVSVKGPSATIKTHHNVGGLPEKMPFALIE
                     PLRDLFKDEVRRIGKELGLPEEILVKHPFPGPGLAVRLLGEITRPHLVTLQEADAIVV
                     EEIRRAGLYSKVWQAFAVLLPVRSVGVMGDGRTYGLTVAVRVVESDDAMTADWVRLPG
                     EVLERISTRIVNEVTGVTRVVYDISSKPPSTIEWE"
     gene            complement(4749..7292)
                     /gene="leuS"
                     /locus_tag="VGR03_04480"
     CDS             complement(4749..7292)
                     /gene="leuS"
                     /locus_tag="VGR03_04480"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV1993566.1"
                     /translation="MAEQNTPGSATAAGSGYDPQKIEEKWQKRWAEARVFESEADPSK
                     PKYYVLEMLPYPSGTLHMGHMRNYTIGDVVARVKRMRGFNVMHPMGWDAFGLPAENAA
                     IKNSTHPREWTNKNIAEFQRVLRRFGFSYDWRREISTCEPEYYRWNQWFFLRMLERGL
                     AYKKKSRVNWCPKCCTVLANEQVVNGGYCWRHEDTLVEAREIEQWFLKTTAYAEQLLD
                     DLKQLEGGWPERVILMQRNWIGKSQGAKVKFAVADVAGAEPIEVFTTRIDTIYGATAV
                     ILAPGHPLLSKLLEASPARAEADAKLAKMRQASVKTEDLATMEKEGFFTGRYAVNPFS
                     GGKTPIWVGNFVLMEYGTGAIMAVPAHDQRDLEFCRKYSLPVRVVVQPLEGETLVEDK
                     MTAAFDEHEQGRLVNSGPYNGLSPEEAIAKMAAFAEQKGFGRAETIFRLKDWGVSRQR
                     YWGTPIPVVYCSKDGMVAVPDKDLPVTLPPNPKLTGMGQSPLASTPEFVNTSCPKCGG
                     PAKREADTMDTFVDSSWYFYRYCDPHNDKAPYDSAKVGYWFPIDQYIGGITHAILHLL
                     YSRFWCKVMRDLGLIKHNEPAARLFTQGMVLKGGVAMSKNRGNVVGAEDMAQKYGADT
                     GRLYTLFAAPPEKDLEWSEESIEGSWRFLNRVYRLVDRHAQVVRGAKSGNADAATASP
                     KEKILQRKAHQTLRRVTQDFETRWHFNSAIALIMELTNEIYLQEPLEKDVRPEVRKEV
                     LELLTLMLAPMTPHLAEELWEMLGHTGGLWTAGWPAFDETLTREDEAEIPVQVNGRLR
                     GKVKVAVGTGEEEVVKLAQADTAIAPHLAGKRIVKRIFVPDKLLNLVVA"
     gene            complement(7299..8504)
                     /locus_tag="VGR03_04485"
     CDS             complement(7299..8504)
                     /locus_tag="VGR03_04485"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEV1993567.1"
                     /translation="MTEQKFSPGMPEQPIIQPTQFDPEDEYRPEPEPEPLPPSWMDGS
                     LRIGIHTSIAGSYLNALESARKLGCNALQIFSASPRMWQGGAARIPEVDAQAFRARRD
                     ELRLGPLVIHANYLINLASEQRMLQTRSIQAFHDEIVRAVALGADFLVVHPGARGEST
                     TSQAISTIVESVKQASKRAPMGGLRILIENTAGMGTAVGARLEEVGEILAGLRNLPVE
                     ACLDTAHLFAAGYDIKSEAGLASTIGQIDGAIGLENVPVIHANDSKIPLGGRVDRHEH
                     IGKGKIGAGAFGRILRHPRFGTSGPEGLAGRAFVAETPIDEPGDDRRNVAMLWELAGL
                     KEQAPEAEKGFSMLTIALQKKIAIHRKSEKKRKDNEESKKTPKGAEKKVRTKKSGAKK
                     SSAKKKKRG"
     gene            8616..>9419
                     /gene="aroF"
                     /locus_tag="VGR03_04490"
     CDS             8616..>9419
                     /gene="aroF"
                     /locus_tag="VGR03_04490"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1993568.1"
                     /translation="MLVVMQSHATEEQIREVCKRIESLGLKAHPMPGSLRTAIGITGN
                     KGAIDLGILESLPGVVECIPVSKPYKLVSRDTKQEDTILRIPTPSGDVIVGGGHIALV
                     AGPCAVETEEQCLAIAERLKKSGARLFRGGAYKPRTSPYSFQGLGEEGLKILSKVRAT
                     HGFGIVTEAIDSESLDLVEEYADVIQIGARNMQNFSLLKRAGRAKKPVLLKRGMAATL
                     DEFLMAAEYILSEGNYNVMLCERGVRTFSDFSRYTLDLAVVPAVKKRSHL"
BASE COUNT         1736 a         3257 c         2365 g         2061 t
ORIGIN      
        1 acctacccgc cgttccgatc ggtgaagtca tgcgcgcctt cggatttgcc gagatcgtcg
       61 agtcgcggca ccctgattac aaaaagggag accgcgtcac gggcctgacg ggccttcagg
      121 aatacgtcat cctcgatgaa gcagccaagc gctccttcca aaaagtgccg aagattccct
      181 ttgtttcaga caccgtcttc ctcggggtgc tgggtatcaa cggcctcact gcctttttcg
      241 gcatggagat cgcagcgccc aagaaaggtg aaacgctggt cgtctccgcc gccgctggcg
      301 ccaccggcag catcgtcggg caaattggaa agattcacgg ttgccgcgtc gtcggcattg
      361 ccggcaccga cgacaaatgt gcctggatca ccaaggatct cggtttcgac gccgcgatca
      421 actacaagca cccggactgg aaggaaaagc ttgccgccgc cactcccaac ggcatcgata
      481 tcgattttga aaacgtcggc ggggaaatca tgcaagaagt cttgaaccgc atgaatctcc
      541 acggccgcgt cgttgtctgc ggcctcatct ccggatacac caaggaagat cccggcctcg
      601 ccagtctcgg caacatcctg gtgaagcgcc tccgcgtcca gggcttcatc atcctggatt
      661 acgcttcgcg cttcatggaa gccgcgacgc agttgggtca gtggaaaatg ttcggcaagc
      721 tgaaagaccg cgagaccatc gtggaaggac tcgaaaaagc gcccgatgcc atcaacatgc
      781 tcttcacggg cgggaacacc ggaaaactca ttgtcaaagt ctgatcggac tactcgaccg
      841 cactgaatta ctggtgcgcg tttctctttc tatcttcgtc caagcgcttc aagaactctt
      901 cgaacttcgg cttttgctcc ggcgtgagga tcgcccgaat ctgttcccgg cccttttgtc
      961 tcgcttcatt gatctgcggg tccgttgact ggtggatggc tttgtattgc gcctgcacgc
     1021 tggcgataat cacttcgagc tgtttctgtt gctcggagga cagatccaat tctttcgtca
     1081 ggcgctgaac tttttgcgcc cgcttggccg cctcggtcat ctgcggcgga gctgccagag
     1141 ccgtgtggtg cgcgaaaacg tagccgatca tcccgccgag cgccgccccc agcaggaaca
     1201 cgacgccgac ccacagggcc gccttccggg aaaccgacat gtcgctcatt gattcttctc
     1261 cgccatgctg atcagcacat catcctggtt catcggctgc tgtggtgcct caaacaggta
     1321 ttcctgggaa gtcgccgcag caggctgctt gttcggcgcc gccgccggtc tttcatacag
     1381 ccacgtgctg cccgccagca acacaaccgc tgaaacccag gcgagccgcg aggcatgacg
     1441 cggaaccgct ccccagagac tcgcgggaag agcaagttcc cgctctcgcg atcgaatcgc
     1501 tcccatcacc ccggctgcaa accacggccc agcctgccct gcgcgagagg caactccctt
     1561 aaagatttcc cgggtggcga gaagatcctg cgcggcttcc cggcagttcc cgcaggctgc
     1621 gaagtgagcg cgctgcgccg cgggccatgc ctcgctcaac tgttcgacac gtatggcttc
     1681 agggcgagcc gctgcggaat cttccagcaa atcccgcaac gtgctgcacg cttcatcact
     1741 cttcttcagc aggttccaca tacccgcctc ctcaccgctc ttgctcttgt cctcaatctc
     1801 cacgcttctt tgcctggctt gccactcggc gccgggcccg aaagagccta accttcaccg
     1861 tattgccatt cagattcaga acttccgcga tctcctcgat ggaaaaccct tccacttcct
     1921 tcaggatcag catcagccgg tcgcgctcat ccagcccttc cagcagccgc tctacgcgct
     1981 cccgcgcttc gagtttctcg ctgatatccg gaccggggtt ttctttttcc cctgaggtga
     2041 gaacctgtct ggcctgttcc tcgctcagat cggattcgta cagcaggggc cgaaccttct
     2101 tctttctcaa taaatcccag cactcattca cggtgatctt atacagccag gtgctgaatg
     2161 ccgctcgctg atcaaacttt ttcagcgaaa aatatgcctt cagaaaaacc tgctgggcga
     2221 tgtcttcgac atcatctcgc cttcgcagaa tcccgcctgc caccgcaaac acgcggtgct
     2281 gatgacgccg caccagctcc tcaaacgcct cctggtcctc tccctgcgcc cggcgaacca
     2341 gctcgcggtc gtccgtcgcg ccaacaggct tggaagcgcg cgcctgtacg gctcccaccg
     2401 ccgggggcgg gtcgaaaggc tccacttttg ggacgtgcgc cataggcgtt tggttacggc
     2461 gattatctcc cggttgcctg aacctgtatc aagtgtttca cttccccacc cgcaaatttg
     2521 tacttgcttc ccatcttagc tacatgaaat aatacctgca tgattattgg tgaccgcctt
     2581 cgcgcactcc gcgaagaaaa gaaactctcc cagggcgaca tcgaaaagcg aacgggtctt
     2641 cttcgttgtt acatttcccg cgtcgagaat ggtcatactg tcccggccat cgaaacgttg
     2701 gaaaaactcg ctcgcgctct cgagtgccca ctctatcaac tcttttatga cggtgaggaa
     2761 ccgccgcagt tgccgaatct tcccaagcgg aaatcgtctg acgacatcgc ctggggcagc
     2821 gtcggtaaag atgcacgctt tctcaacaag cttcgccgct tgctgagcaa agtggaagaa
     2881 ggggatcgca agctcattct ctacatggcc cagaaaatgg ccaaccgcta gtcctgcgat
     2941 cggacttccc cggttgtcgc tgtactgtct catgacgtgt tacggaacag gccgcgcatg
     3001 cccgctgcgc ggcctgtttt ctttccagcc tctagatttt cagcaaggtt gtccgctggt
     3061 cgccccaggt cagctctcct gctcattgcc aggccctctc gcagaactat tcccattcaa
     3121 tcgtgctcgg aggtttggag ctgatatcgt aaaccaccct cgtcacgccg gtcacttcat
     3181 tgacgatccg ggtcgagatg cgttctagta cttccccggg cagccgcacc cagtccgccg
     3241 tcatggcgtc atcggactcc actaccctca cggccacggt cagcccatag gttcgcccgt
     3301 cgcccatcac cccgacgctc cgcaccggga gaagcaccgc aaatgcttgc cacaccttgc
     3361 tatacagccc cgcgcggcga atttcctcaa ccacgatcgc gtcggcctcc tggagagtta
     3421 ccagatgtgg acgggtgatc tccccgagca gccggacggc cagccccggc cccggaaacg
     3481 ggtgtttcac cagaatttct tccggcaggc ccagttcctt gccaatccgg cgcacctcgt
     3541 ccttaaagag gtccctcagc ggctcgatca gcgcaaaagg catcttttcc ggcaatcctc
     3601 cgacgttatg gtgcgttttg atggtcgccg aaggtccctt cacggaaacc gactcgatca
     3661 cgtctggata gagggttccc tgcaccaggt atcctatctc cccgtgcgcc tcccccgcct
     3721 gcagcttctg cgcttcctcc gcaaagacgg cgatgaactc tctgccgatg cgcttgcgtt
     3781 tctcctcggg gtccgtaacc cctttcaatt gagcaagaaa gcgttccgaa gcgtccacgc
     3841 cgatgacgtg gagtttcaac cggtcccgca gcatctccag cgtctgctca aactcgtttt
     3901 tcctcagcat ccccgtattc acgaagatgt tcgtcagtcg ggttcccatg gcccggtgta
     3961 ccagcactgc cgcaaccgtt gaatccacgc ccccgctcag cccgcagatc gcccacttat
     4021 ttcccacttt ctctcggacc gccgccgtcg tctcctcgat gaacgccgct ccgctccact
     4081 tcggctccgc gtcacacacg cggaacagaa aattccgcag cagttcggtc ccgtgttccg
     4141 tgtggttcac ttccgggtga aactcgaccg catagatctt cttctccaca ttttccacgg
     4201 cgccaatagc attttccgtt cgcgcggtag tgcgaaatcc ttccggaagc gcccgcacgt
     4261 ggtcgccgtg gctgttccac acgcgaagag agcccggaat ccccgtgaac agctcggatc
     4321 ctccggtttt tggggcatcc ccaagactca attgcgcgcg gccatattcc cgccgctccg
     4381 ccttctctac cgtgccgccg agtgtgtggg tgatccagtg catcccgtag caaatgccta
     4441 gcaccggaat tcccagcgcc agcactttcg gatcgcactt cggcgcatcg gcgtcataaa
     4501 ccgagctcgg cccgccggag agaatgattc ccgcaggttc cagcttgcgg atttcctcga
     4561 tggaagtggt gcatggcaaa acggcggaga acacctgctg ttcgcgaatg cggcgcgcaa
     4621 tcagttgcgt atactgcccg ccgaagtcga gaaccaccac gccgccgagt tccgccttca
     4681 tcctgcctcc ctgctctcgc gcttgctccg tcacctgact tgccgcgctc tccatcatgc
     4741 catccccctt acgccacgac gagattcaat aatttgtccg gcacgaaaat acgtttcacg
     4801 atacgtttgc ccgcaaggtg cggcgcaatc gccgtgtccg cttgcgccaa ttttacgact
     4861 tcttcttccc cggttcccac ggcgactttc accttgcccc gcagccgtcc gttcacctgc
     4921 acggggattt cggcttcgtc ctcgcgcgtt aacgtctcat caaatgccgg ccagcccgcc
     4981 gtccacaatc cgccggtgtg tcccagcatt tcccaaagct cttccgccag gtgcggcgtc
     5041 atcggcgcca gcatcagcgt cagcagttcc agcacttcct tgcgaacctc cggccgcacg
     5101 tctttttcca gcggctcctg caaataaatc tcgttggtca gctccatgat cagcgcaatc
     5161 gcggaattga agtgccagcg cgtctcgaag tcttgcgtca cgcgccgaag tgtctggtgc
     5221 gctttgcgct gaagaatctt ttccttcggt gaagccgtcg ccgcatcggc gtttccgctt
     5281 tttgcgcccc gtaccacttg agcgtgacgg tccaccagcc ggtacacgcg attcagaaac
     5341 cgccaagagc cttcaatgct ctcctcgctc cactccaaat ctttttccgg cggcgctgca
     5401 aagagggtgt acagccgccc ggtgtccgcg ccgtacttct gcgccatatc ttccgcgccg
     5461 acgacgttgc cccgattctt agacatggct acgccgccct tgagcaccat gccttgcgtg
     5521 aagagccttg ccgcgggttc gttgtgcttg attaacccga ggtcgcgcat caccttgcac
     5581 cagaaccgcg aatatagcag atgcagaatt gcgtgtgtga tccccccgat gtactggtcg
     5641 atcggaaacc agtagccgac cttcgcagaa tcgtaaggcg ctttgtcgtt gtgtggatcg
     5701 cagtagcggt agaaatacca cgacgaatcc acaaacgtat ccatcgtatc tgcttcgcgc
     5761 ttcgcaggtc cgccgcactt cgggcagctc gtattcacga actccggcgt agacgctagc
     5821 ggcgattggc ccatgcccgt cagcttcgga ttcggcggca gcgtcaccgg caaatctttg
     5881 tccggcaccg ccaccatgcc gtccttcgag caatagacga ccgggatcgg cgtgccccaa
     5941 tacctctgcc gcgaaacgcc ccagtccttc aacctgaaaa tggtctcggc gcgcccgaat
     6001 cctttttgtt ccgcgaaagc cgccatcttc gcaatcgctt cttccggcga aagcccgttg
     6061 tacggtcctg aattgacgag ccttccctgc tcgtgctcgt caaacgccgc cgtcattttg
     6121 tcttcgacta atgtttctcc ctcgagcggc tgaaccacca cgcggacggg aagactgtac
     6181 ttgcggcaaa attccaggtc gcgctgatcg tgtgccggaa cggccatgat cgcgcccgtg
     6241 ccgtattcca tcagcacgaa attcccgacc cagataggag ttttcccgcc gctgaaggga
     6301 ttgacggcgt accgtcccgt gaagaagcct tccttctcca tggttgccag atcttcggtt
     6361 ttcaccgatg cctggcgcat cttggccagc ttggcatctg cttccgcgcg cgctggcgaa
     6421 gcctccaaca gctttgacag cagcgggtgc cccggcgcca ggatcactgc ggtcgcgcca
     6481 taaatcgtgt caatgcgcgt ggtaaaaact tcgatcggtt ccgcgcccgc cacatccgcc
     6541 acggcgaact tcaccttcgc gccctgcgac ttcccaatcc agttccgctg catcaggatg
     6601 acgcgttccg gccatccgcc ctcgagctgc ttcaaatcat ccagcaactg ctccgcatac
     6661 gccgtcgtct tcaggaacca ctgctcgatt tcgcgcgctt ccaccagcgt gtcttcatgc
     6721 cgccagcagt aaccgccatt caccacttgc tcgttcgcca gcaccgtgca gcacttcgga
     6781 caccagttca cgcggctctt ttttttgtat gccagcccgc gctccagcat gcgcaggaag
     6841 aaccactggt tccagcggta atactccggc tcgcaagtcg aaatctcccg ccgccagtcg
     6901 tagctgaacc caaagcgccg cagtacgcgc tgaaattccg caatattttt gttggtccac
     6961 tcgcgcggat gggtgctgtt cttgatcgcc gcgttttccg ccggcaggcc gaaggcatcc
     7021 cagcccatcg gatgcatcac gttgaatccg cgcatgcgct tcacgcgcgc cacgacgtcc
     7081 ccaatcgtgt agttgcgcat gtggcccatg tgcagcgtgc cgctcgggta cggcagcatc
     7141 tccagcacgt aatacttggg cttcgaaggg tccgcctccg attcgaatac gcgcgcttcc
     7201 gcccagcgct tctgccattt ctcttcaatt ttctgcggat cgtatcccga ccccgccgct
     7261 gttgcgctgc ctggtgtgtt ctgttccgcc acgcccgctt atcccctttt cttcttcttc
     7321 gctgaactct tcttcgctcc gctcttcttc gtccgcactt tcttctctgc gcctttcggc
     7381 gttttcttcg actcttcgtt atctttcctc ttcttttctg actttctatg tattgcgatc
     7441 tttttctgca gcgcgatcgt gagcatcgag aagcccttct ccgcctccgg cgcctgctcc
     7501 ttcaatcccg ccagctccca caacatcgct acgttccgcc tgtcatcccc cggctcgtcg
     7561 atcggcgtct ccgccacaaa cgcccgcccc gccaatcctt ccggccccga cgtgccgaat
     7621 cgcggatgcc gcaaaatcct cccaaacgcc cccgctccaa tctttccttt tccgatatgt
     7681 tcgtgccggt ccactcgccc gcccagcgga atcttcgaat cgttcgcgtg aatcaccggc
     7741 acgttctcga gcccaatcgc gccgtcgatc tgaccgatcg tcgaagccaa tccggcctcg
     7801 ctcttgatgt cgtatcccgc ggcaaaaagg tgcgccgtat ccaggcacgc ctcgaccggc
     7861 aagttccgca gccccgccaa aatctctccg acttcttcca gccgcgcccc aaccgccgtt
     7921 cccatccccg cggtgttctc gatcagtatt cgcaatccgc ccatcggcgc acgcttcgac
     7981 gcctgcttca cggactccac gatcgtggaa atcgcctgcg acgtcgtcga ctcgcctcgc
     8041 gcccccgggt gcaccaccag aaaatccgct cccagcgcca ccgcgcgcac gatctcatcg
     8101 tggaatgcct gaatcgaacg cgtctgcagc atccgctgct cggacgccag attgatcagg
     8161 tagttggcat gaatcaccag cggcccgagc cgcaactcat cccgccgcgc ccgaaacgcc
     8221 tgcgcgtcca cttccggaat ccgcgccgcg ccgccctgcc acattcgcgg gctggccgag
     8281 aagatttgca gcgcgttgca acccagcttg cgcgccgatt ccagcgcatt cagatagctt
     8341 cccgcgatag acgtatgaat cccgattcgc aggctcccat ccatccacga cggcggtaga
     8401 ggttcgggct caggttccgg gcgatattcg tcttctgggt cgaattgcgt cggttggatg
     8461 atgggctgtt caggcatgcc tggtgagaat ttctgttccg tcataaacca gctattctat
     8521 cacacgcgcc aaacggctgg tcccgcgtcc atttgctgcc cagcagcagc ttgacttaaa
     8581 ctgaatgttc accaaccgtg ctgggggaac cctccatgct tgtcgttatg cagtcgcacg
     8641 ccaccgagga acagattcgc gaagtctgca agcgcattga aagcctcggc ttgaaggccc
     8701 accccatgcc ggggtccctc cgcaccgcca tcggcatcac cggcaacaaa ggcgccatcg
     8761 acctcggcat cctcgaatcc ctccccggcg tcgtcgagtg catcccggtc agcaagcctt
     8821 acaaactcgt cagccgcgac accaagcagg aagacactat ccttcgaatt cccacgccct
     8881 ccggcgacgt catcgtcggc ggcggtcata tcgccctcgt cgccggcccc tgcgcggtcg
     8941 aaaccgaaga gcaatgcctc gccattgccg aacgcctcaa gaaatccggc gcacgcctct
     9001 tccgcggcgg cgcctacaag ccccgcactt ctccgtacag cttccagggc ctcggcgaag
     9061 aaggcctcaa gattctttcg aaagttcgcg ccacccacgg ctttggcatc gtcaccgaag
     9121 ccatcgatag cgagagcctt gacctcgtcg aggaatacgc cgacgtcatt cagatcggcg
     9181 cgcgcaacat gcagaatttc tccctgctca agcgcgccgg ccgcgccaaa aagcccgttc
     9241 ttctcaagcg cggcatggcc gccacgctcg acgagttcct catggccgcc gagtacatcc
     9301 tctccgaggg caattacaac gtgatgcttt gcgagcgcgg cgtgcgcacc ttctccgatt
     9361 tttcgcgcta cacgctcgac ctcgccgtcg ttcccgcagt aaaaaaacgc agccatctc
//
LOCUS       DASYVO010000135         4167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_201820, whole genome shotgun sequence.
ACCESSION   DASYVO010000135 DASYVO010000000
VERSION     DASYVO010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201820"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VGR03_04495"
     CDS             complement(<1..598)
                     /locus_tag="VGR03_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV1993569.1"
                     /translation="MSSSVKTVVRAYQNYINGQWVKSSSGEMFPVYDPSTEEVIAHVA
                     SGTAADVDSAVKAARAAFDSGPWPQTSAAERGRLLYKLAEKIRANAAPLAELECRNSG
                     KPIVEAEYDIADAATCFEYYAGLANKVLGHVNPVPANALSFTLREPVGVAGQIIPWNY
                     PLVMASWKLAPAIAAGCTCVLKPAEQTPLTMMEVANWFE"
     gene            complement(614..2068)
                     /locus_tag="VGR03_04500"
     CDS             complement(614..2068)
                     /locus_tag="VGR03_04500"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV1993570.1"
                     /translation="MANEVSTAPAAPVAQEAPHLRRVLGRWDLVLLFVVAVFNLNVVP
                     SIAANGGVTVWLWLISLVLFFWPQGIAVIELAHRFPGEGGVYLWAKEVFGDFHGFLSG
                     WCYWTNNMMYVPTIMLYFVGVFVFVLGAGHESLADNKSFTLTASLVLLLLLVVLNILG
                     LGVGKWLNNLGAIGTFVAAAVLIGLGIVIWLRFGTTVTSADFRIPANPRFVLNSFGVI
                     CFGLVGLELASVMGDEIENPQKTLPGAVAWGGVLSGLLYIGATLTLLIAVSKSDISVL
                     QGIVQAVGHMASRVGVSWIVAPFAFLLSISIAGIGSAWLGGSARIPFVAGLDSYMPSW
                     LGKIHPKYATPYAALIVHAVVSMILVVINFSFTGAGVQETFQKLLSLAVVLQLIPFLY
                     MFGALLKIAFSDSFAKGRYGRTTLILAGFSGFTTTGLGIALAFFPAQQIKSLRSYEIW
                     MFGGTLFFIGLAAFFFFVYGRRKAARKLAAAPAL"
     gene            complement(2103..3485)
                     /locus_tag="VGR03_04505"
     CDS             complement(2103..3485)
                     /locus_tag="VGR03_04505"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEV1993571.1"
                     /translation="MHDALQKEIATYEKRTPKSAAAHKRALERIPLGVASNYRHYEPY
                     PIFVQDGKGSRIHDVDGNEYIDHNLCFGALMAGHCHPAVVKAVEQKLHEGTTFGMPHE
                     QEWELAEEICKRYPVDMVRFGSSGTEVTMHACRVARAATGRDKILKFEGSYHGLHDTA
                     LVSVKPKAEEYGDPDAPNSVPGGGGVPKAALEYVVVASFNNLKSVENRFKQYPGQIAG
                     IILEPILMNVGLCLPQHGFLQGLRDVCTRNGALLIFDEVKTGAKLAWGGASDFFDVKP
                     DIICLAKSIGGGLPLGAFGSSRAVMDLISQHKVFHGGTYNTNRVAMAAGLAAFREVLT
                     RENYAHVGKLSKTLTEGYRKIVAKAGLQAYVASAGVNGALMLYAKEIQNYRDWTKIDV
                     DLWRHYWFAMVNRGVLAQPYWWDEQWTISVQHTEADIDKHLAVFEEVAPALAKAQQER
                     GLAFVGAAGH"
     gene            complement(3714..>4167)
                     /locus_tag="VGR03_04510"
     CDS             complement(3714..>4167)
                     /locus_tag="VGR03_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993572.1"
                     /translation="TTLQTGFPIRLESSNDSELENSFDFELPGKPDLIAPFRTFDPRT
                     HGGLYFDPNSFALPVQDANSTPLQLLGNAPRTICCGPGISNFDFSVQKVTRMGEKTHL
                     EFRAEFFNIFNHTQFLNPDGSITDGADFGRVKRTREPRQVQFALKLFF"
BASE COUNT          895 a         1230 c         1236 g          806 t
ORIGIN      
        1 cttcaaacca gttggcgact tccatcatgg tgagcggggt ctgctcggca ggcttgagga
       61 cgcaggtgca gccggcggcg atcgccggag cgagcttcca cgaagccatc accagcggat
      121 agttccaggg aatgatctgt ccggcgacgc caacgggttc gcgcagagta aaactcaggg
      181 cattcgcggg caccggattg acgtgcccga gaactttgtt cgcgaggccg gcatagtatt
      241 cgaaacaagt cgcggcgtcg gcgatgtcgt actccgcctc gacgatgggc ttgcctgaat
      301 tgcggcattc cagctcggcg agcggtgcgg cgttcgcccg gattttctca gccagcttgt
      361 agagaaggcg gccgcgctcg gcggcgctgg tctgcggcca cgggccggaa tcaaacgcgg
      421 cgcgcgcggc tttcacggcg ctatccacat ctgccgctgt gcccgaggcg acgtgcgcga
      481 tgacttcttc ggtggaagga tcgtagacgg gaaacatctc gccggaagaa ctcttgaccc
      541 attgtccgtt gatgtagttt tgataggctc gaacgactgt tttcacgctg ctggacataa
      601 ctgcctccca aattcaaagc gcgggcgccg ctgccagttt ccgcgcggcc ttgcgcctgc
      661 cgtacacgaa gaagaaaaac gccgcgaggc cgataaaaaa caacgtgcct ccgaacatcc
      721 aaatctcgta agaccggagc gacttaatct gctgcgcggg gaaaaatgcc agggcgattc
      781 cgaggcccgt ggtggtgaat ccactgaagc cggcaaggat cagtgttgtt ctgccgtagc
      841 gcccttttgc aaaagaatca ctgaaggcga tcttcaacag cgcgccaaac atataaagaa
      901 acgggatcaa ttggagcacc acggcaagag acaacaactt ctgaaacgtt tcctgcactc
      961 ccgcgccggt aaaagagaag ttgatcacca cgagaatcat cgagacgact gcgtggacga
     1021 ttagcgcggc gtacggcgtg gcgtacttcg ggtggatctt gccaagccag gacggcatat
     1081 aggaatcgag gcccgcaaca aaaggaattc gcgccgaacc gccgagccag gcggagccaa
     1141 tgcccgcgat cgagatgctc aaaaggaatg cgaagggtgc cacgatccag ctaactccaa
     1201 cgcggctggc catgtggccg acggcttgca cgatgccttg cagaacgctg atgtcgcttt
     1261 tgctcacggc gataagcagc gtcagcgtcg ctccgatgta aagcaaaccg gatagaacgc
     1321 cgccccaggc gacggcaccg ggaagagttt tctgaggatt ctcgatttca tcgcccatga
     1381 ccgaggcgag ttccagacct accaggccga aacagatcac gccgaaggaa ttcagaacaa
     1441 aacgggggtt tgcgggaatc cggaagtccg ccgacgtcac tgttgtgccg aaccgcagcc
     1501 agatcacaat tcccaaacca ataaggacag ccgccgcaac gaacgttccg atcgcgccga
     1561 gattgttaag ccacttcccg acgccgagac cgagaatatt cagcacgacc agcaggagta
     1621 acagaaccag ggacgccgtt agcgtgaagg atttgttgtc cgcgagcgat tcgtgcccgg
     1681 ctcccagaac gaacacgaac acgccgacga aataaagcat gatcgtcggc acgtacatca
     1741 tgttgttcgt ccagtagcac cagccggaga gaaaaccgtg gaagtcgccg aagacttcct
     1801 tggcccagag gtacacgccg ccttcgcccg gaaagcgatg cgcaagctca atcacggcga
     1861 tgccctgcgg ccagaaaaac agtacgagcg agatgagcca cagccacacc gtcacgccac
     1921 cattggcggc gatggacgga acgacattga ggttgaaaac ggcaacaaca aaaagcagaa
     1981 cgaggtccca ccgtcccagc acccggcgca ggtgcggcgc ttcttgcgcg acaggcgctg
     2041 ccggcgcagt ggagacctcg ttcgccaatg gcgttctctc ctgactcccg tgctgaagcg
     2101 gactagtgcc cagccgctcc gacaaatgcc aggcctcgct cctgttgcgc cttcgcaagc
     2161 gccggcgcga cctcttcgaa taccgctaga tgcttgtcga tatccgcctc ggtatgctga
     2221 accgagatcg tccactgctc gtcccaccag tagggctgcg cgagcacgcc tcgattgacc
     2281 atggcgaacc agtagtggcg ccagagatcg acatcgatct tggtccagtc gcggtagttc
     2341 tgaatctcct tcgcatagag catcagcgcg ccgttgacgc ctgcgctggc gacatacgcc
     2401 tgcaagccgg ctttcgcaac gatcttgcgg tagccctcgg tgagagtctt gctgagcttg
     2461 ccgacgtgcg cataattctc gcgcgtgagg acctcgcgaa acgccgcgag tcccgcggcc
     2521 atggccacgc ggttggtgtt gtaggtgccg ccatggaaga ccttgtgctg cgagattaga
     2581 tccatcaccg cgcggctcga gccgaaggct cccagcggca agccgccgcc gatggacttg
     2641 gccaggcaga tgatgtccgg cttcacgtcg aaaaagtcgc tcgcgccgcc ccacgccagc
     2701 ttggcgccgg tcttcacctc gtcgaagatc agcaacgccc cgttcctggt gcaaacatcg
     2761 cgcagcccct gcaggaatcc gtgctgcgga aggcacagtc cgacgttcat gaggatgggc
     2821 tcgagaatga tgccggcgat ttgtccggga tactgcttga aacgattttc gacgctcttc
     2881 agattgttga agctggcgac aacaacatat tcgagcgcgg ccttcggcac gccaccgcct
     2941 ccgggaacgg aattgggcgc gtccggatcg ccgtactctt ccgccttggg cttgacgctg
     3001 acgagcgcgg tgtcatggag tccgtggtac gagccttcga acttcagaat cttgtcgcgg
     3061 cccgtcgcgg cgcgcgcgac gcggcaggca tgcatggtga cttccgtccc gctggaaccg
     3121 aaccgaacca tatccaccgg atagcgcttg caaatctctt cggcaagctc ccattcctgc
     3181 tcgtgcggca tcccgaaggt cgttccttca tgcagcttct gttcgacggc cttcacgacg
     3241 gcgggatggc aatgccccgc catgagagcg ccgaagcaga ggttgtggtc gatgtactcg
     3301 ttgccgtcga cgtcgtgaat gcggctgccc tttccatcct gcacgaaaat cggatagggc
     3361 tcgtagtggc ggtaattgct ggcaactcca agcgggatgc gttccagcgc gcgcttgtgc
     3421 gctgccgccg atttcggggt gcgcttttcg taggtggcga tttccttctg tagtgcgtcg
     3481 tgcatttgag tactccgtga ttgcaggtct attccctgcg gtcagtatcg gtgtggaaaa
     3541 cctggcgatg ctcttgcgcg gcttttgcct tacttctagt gtacaccctg caattccaat
     3601 gaagaccgca atggcctaac gggtggctct atccgcgcaa ttttttcaaa cgtgcgaagg
     3661 cgcggccctt gcgagccgcg ccttctgaac tgtcgtcgat ctcctctggc ttgctagaag
     3721 aacagcttca gtgcgaactg cacctgccgc ggctcccgag tgcgcttgac gcggccaaaa
     3781 tccgctccgt ccgtaatgct gccgtccggg ttcagaaact gcgtatgatt aaagatgtta
     3841 aaaaactcgg cgcggaactc gaggtgagtt ttttctccca tgcgtgtgac tttctgcaca
     3901 gagaaatcga agttagaaat cccaggtccg caacagatgg tccgcggcgc gttgccaagc
     3961 aactgcagtg gcgtcgaatt ggcatcctgg acggggagag cgaaggaatt gggatcgaag
     4021 tagaggccac cgtgggttct gggatcaaac gtgcggaatg gggcaatcag atccggttta
     4081 ccaggtagct caaagtcaaa gctgttttcc agttccgagt cgttgctcga ctccagtcga
     4141 atggggaaac cggtctgcaa ggtagta
//
LOCUS       DASYVO010000136        11427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_46960, whole genome shotgun sequence.
ACCESSION   DASYVO010000136 DASYVO010000000
VERSION     DASYVO010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46960"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..2098)
                     /locus_tag="VGR03_04515"
     CDS             complement(167..2098)
                     /locus_tag="VGR03_04515"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEV1993573.1"
                     /translation="MDKSVDPCVDFYRYACGGWQSKNPIPSDQTSWSVYAKLYQDNLN
                     FLHGILEQAASDTNHRDAVTQEVGDYYAACMDDAKVERRGISAVQSQLEAIAKLKSTR
                     ELPPLIARLQLVYGRSILFAAGSTQDPDNSEEIIAELDQGGLGLPDRDYYTKTDDKSK
                     QTRERYAQHVRKIFELMGDRPETTMQNADTVIRLETALAKASLTRVERRDPYKLKHKM
                     TSTGLNELAPNFDWKTYYREAQYPNFEILNVDTPDFFKEVNARLAAEPIENWKTYLRF
                     HVVDSASPYLSSRFVQENFDFYRNYLRGAKEQQPRWKRCVTYTNHNLDEALGQVYVAK
                     VFSPELKQSTLDIVKRVEDAMAQRIRQLDWMSPETKEQALTKLQGIRNKIGYPDKWRD
                     YRSVKILRDDFEGNMQRAAEFEAHRQINKIGKTVDHGEWQIPPPTVDAYYDPQMNDIN
                     FPAGVLQPPLFDAKMDDAPNYGDTGGTIGHELTHGFDDEGSQFDAKGNLRNWWTKEDR
                     ERFESRTKCVVDQYAQYVVVDEVHINSKLTLGEDVADLGGEILAYMAWKNATKDLSLQ
                     SIDGLTPEQRFFIGFAQWDCANERPEDLRVRAVTDPHSPAVYRINGVVENMPEFSRAF
                     SCKQGQPMTKPPEKVCKVW"
     gene            complement(2401..3168)
                     /locus_tag="VGR03_04520"
     CDS             complement(2401..3168)
                     /locus_tag="VGR03_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEV1993574.1"
                     /translation="MSPHAESDRLRVILVAPRNPLNIGAAARAMSNFGFLHLRVVNPY
                     ELAFREARSAVGAAPLLACAEEFKTVAEAVADCTLVVGTTAVGRRQPQHPVRRLDQAA
                     RLLHKHLAGSRVALLFGSEKRGLSNEDLSHCHWLLRIPTREEHRSMNLGQAVAVCLYE
                     LARDSQAAPKPEKHIAATAADLDRLTALLLDALCSSGYLKSNPSGSMKRRSATPTEEK
                     IRRLVRRLHLSATDADLTLGILRQIFWKLRFRNDPFG"
     gene            complement(3276..3476)
                     /locus_tag="VGR03_04525"
     CDS             complement(3276..3476)
                     /locus_tag="VGR03_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993575.1"
                     /translation="MDHIPTRLTLILMIFVASSPYLLLLESGIAGYRGFSSWMRYAAL
                     VPTVLSGIGLVAAFLVPRLLRR"
     gene            4028..5782
                     /locus_tag="VGR03_04530"
     CDS             4028..5782
                     /locus_tag="VGR03_04530"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEV1993576.1"
                     /translation="MATPLETWVEEAAKLTKPERVVFCDGSEAEYQRMIEEMLHAGDS
                     YTLNEKTYPNCHLHRSSPNDVARTEHLTYICSPDKEDVGPTNNWMDPGAAKHKVGALL
                     DGAMRGRTMYVVPYVMGPVNSPISKVGVEVTDSPYVVASMRIMSRMGKVAMERLGASS
                     EFVPGVHSLGDLDPMRRYIMHFPQEKLIWSVGSGYGGNALLGKKCFALRIASVMAREQ
                     GWMAEHMLILGLESPGGKVTYMAAAFPSACGKTNLAMMVSALESQGYRVWTIGDDISW
                     MMIGEDGYLHAINPEAGFFGVAPGTGMKTNQNVMGALRKNTLFTNVAMTSDREPWWEG
                     IGSEAPAGLINWKGEAWDPSKGAAAHPNSRYTVPAQQSPSISPKWEALEGVPISAFIF
                     GGRRTRVAPLVYESFDWQHGVFVGATMASETTAAATGDVGISRRDPMAMLPFCGYNMS
                     DYFGHWLEMGKKIPIPPKIFHVNWFRKGADGKFLWPGYGENVRVLKWILERVEGRGAA
                     QETPIGYVPAKKGLTLEGMKISDEAVNELLRVHPEDWELEMEDSKQFFGKFGERLPRQ
                     ISEEHEKLARRFQRVVTA"
     gene            complement(5799..6341)
                     /locus_tag="VGR03_04535"
     CDS             complement(5799..6341)
                     /locus_tag="VGR03_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993577.1"
                     /translation="MGHQLKSISKAGIAEAIAKVELYRYLNEPEEAESICRDILAVDP
                     GHQLARRMLGLAITDQFIGAASDRYAEVQSIFQALREAYERFYYTGLLHERRAKVQLL
                     VGYAPHTLLPLVQEAMLCFAEAEKIRPAGNDDSILRWNRCVRLLESWPDFHFEREPAA
                     FDAEDMPPISSPPRRAGGFH"
     gene            complement(6440..7171)
                     /locus_tag="VGR03_04540"
     CDS             complement(6440..7171)
                     /locus_tag="VGR03_04540"
                     /inference="COORDINATES: protein motif:HMM:NF009052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEV1993578.1"
                     /translation="MSEATLRVFRGDSNGGQTIDYRVPVAPGMVVLDALHYIQGHLAP
                     DLAVRWNCKAGKCGSCSAEVNGRPRLTCKTRMDDLPQDQPITVQPMKSFPRIKDLVTD
                     VSWNYRVNRKIPAFEPRPGVAWKISQLDVDRVQEFRKCIECFLCQDVCHVLREHDKKE
                     QFAGPRFFVRVAGLEMHPLDGGDRSALLRDQLGLGLCNITKCCTEVCPEEIRITDNAI
                     IPLKERVVDEFYDPLTMLFRKIRGR"
     gene            complement(7168..7560)
                     /locus_tag="VGR03_04545"
     CDS             complement(7168..7560)
                     /locus_tag="VGR03_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993579.1"
                     /translation="MDSQWKALAPAEKNREYLALLSYLPLKKYRAIPSFLRFSFQIQK
                     QLRGTPGVIGYSLRAKVLSRNFWTLSVWEDEKALMDFVAQLPHGEAMKAMTPHMGPSK
                     VTQWKVAGSALPLRWEEAIQRSQHGVQT"
     gene            complement(7607..9328)
                     /locus_tag="VGR03_04550"
     CDS             complement(7607..9328)
                     /locus_tag="VGR03_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEV1993580.1"
                     /translation="MTANYETREHDVLIIGAGGAGLRAAIEALAQGASVGVVCKSLLG
                     KAHTVMAEGGIAAAMANVDAADNWKTHFRDTMSGGKLLNNWRMAQLHAQESPDRVREL
                     EQWGALFDRTENGDILQRAFGGHTFKRLCHVGDRTGLEMIRTLQDRGVQQGIDVYMEC
                     TVTRLLTDGGRVVGAFAYWRETGRLIVFKAKSIVIATGGIGKAWRITSNSWEYTGDGL
                     ALAYEAGAELMDMEFVQFHPTGMVWPPGVQGILVTEAVRGEGGILRNKAGERFMEKYD
                     PKRMELSTRDVVARSIYTEVKEGRGTEHGGAYLDISHQPAEYVKKKLPSMYHQFKELA
                     DVDITKGPMEVGPTCHYMMGGIRVDAETAQSTLPGLFAAGEAAAGLHGSNRLGGNSLS
                     DLLVFGRRAGLGAAEYAKRASAASLDNAQIEQAERELLAPFSNTGAESPYAVHRDLQE
                     VMHDLVGIYRTEEDLKTALVELQKLRARAAKAGVEGSRLFNPGWHLSYDLKAMLIVSE
                     AVTQSALVRTESRGAHSRIDYPNLDSAWEKKHNIIIRESGAMKRSESPIPEMPGELKQ
                     ILAEDKS"
     gene            complement(9325..10083)
                     /locus_tag="VGR03_04555"
     CDS             complement(9325..10083)
                     /locus_tag="VGR03_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993581.1"
                     /translation="MSGSPTLLPESRLGSTVRRDAWWIEIIPIIIMMGGFSIYATVRA
                     FENAYYSWGPYLSPFYSPLIDPQHHWWPFSPALLILVGPLGFRATCYYYRKALYRAFL
                     LDPPACAVGEPLRRDYRGETAFPFILQNVHRYFFYLAVLFIAFLWHDAFRAFFFDGHF
                     GAGVGTIVLSLNVSLLSLYTFSCHSLRHLVGGKLDCFSCATFGRSRFAAWRVSTFLNE
                     LHMLFAWCSLVSVGFADFYVRMVASGAIRDLRLL"
     gene            complement(10169..10444)
                     /locus_tag="VGR03_04560"
     CDS             complement(10169..10444)
                     /locus_tag="VGR03_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993582.1"
                     /translation="MSAEAPGHAPIMPLRDLVRLYRSHAGKFGEPVALSAFGLTNEET
                     GRLFSGYDEDYHISRFFQFSEAAGEKFSINGIPATHVSLDAEIETIL"
     gene            10623..11096
                     /locus_tag="VGR03_04565"
     CDS             10623..11096
                     /locus_tag="VGR03_04565"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEV1993583.1"
                     /translation="MSIAVGQTAPDFTLLNQEKKEVKLSDFAGRKNVVLVWYPLDWSP
                     TCTNEHACFVNEMKSFETLDAEVLGVSVDSVWSHKAYAEKMGIKYSLLADFHPRGAMS
                     EKYGVYLADKGITGRAIAIVNKTGKVAWFKDYDIPVVPDLKEVAAALGQVKAATA"
     gene            complement(11198..>11427)
                     /locus_tag="VGR03_04570"
     CDS             complement(11198..>11427)
                     /locus_tag="VGR03_04570"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV1993584.1"
                     /translation="KMVEWGMKPLDAIQAATVNAADLLGWAEKIGALETGHYADLIAV
                     SGDPASDVRVLESVKFVMKGGTVVRNDYAAK"
BASE COUNT         2506 a         2900 c         3742 g         2279 t
ORIGIN      
        1 gtatggttat ctgggctacc cgacggcgtg gatgatgttt caactgcaga acgacatcct
       61 ggcgcacggc gcgttcgtta acggcaccgg agagatgttt tccgagacga agaattggca
      121 gctggttgcc agcaacgttc cttagacttc cttgaagcaa gaaaacctac cagaccttgc
      181 aaaccttctc tggtggtttg gtcatcggct ggccctgttt gcacgagaag gcgcgcgaga
      241 actccggcat attttcgacc acaccattga tgcggtacac cgccggcgaa tgaggatccg
      301 tcaccgcacg cacacggagg tcctccgggc gctcgttggc gcagtcccac tgggcaaaac
      361 cgatgaagaa gcgctgctcg ggagtgagcc cgtcgatgga ctgaaggctc aagtccttag
      421 tggcgttttt ccaggccatg taggctagaa tttcgccgcc caggtcggcc acgtcttcac
      481 ccagagtgag cttgctgttg atgtggacct cgtccacgac aacgtattgc gcgtattggt
      541 cgacaacgca cttggtccga ctttcgaacc tttcacggtc ttccttggtc caccaattcc
      601 tcagatttcc tttggcgtcg aactgactgc cttcgtcgtc gaagccgtgg gtcagttcgt
      661 gcccgattgt gccgccggtg tcgccatagt tgggagcgtc gtccatcttg gcatcgaaga
      721 gcgggggctg cagaacgcca gcggggaagt tgatgtcgtt catctgtgga tcgtagtagg
      781 cgtccactgt gggcgggggt atttgccact cgccgtggtc cacggtcttg ccaattttgt
      841 tgatctggcg gtgggcttca aattcggcag cgcgttgcat gttgccttcg aaatcatcgc
      901 gcaggatttt cacggagcga tagtcgcgcc atttgtcggg atagccaatt ttgttgcgaa
      961 taccctgcag tttggtgagg gcctgctcct tggtctcggg actcatccag tcgagctggc
     1021 gaatgcgctg ggccatggcg tcttcgacgc gctttacgat atccagcgtg ctttgcttca
     1081 gttcggggga aaaaaccttg gcgacataca cttggccgag agcttcgtcg aggttgtggt
     1141 tggtataggt gacgcaccgt ttccagcgcg gctgttgctc ctttgccccg cggagatagt
     1201 tacggtagaa atcgaaattc tcctggacga aacgggacga gaggtaaggg gaagcggaat
     1261 cgaccacgtg aaagcgcaga taggttttcc agttctcgat cggctcggcc gccaagcgtg
     1321 cattcacttc cttgaagaag tccggcgtgt ccacattgag aatttcaaag tttggatact
     1381 gcgcctcgcg gtaataagtt ttccagtcga agttcggggc caattcgttc agacccgtgg
     1441 atgtcatctt atgcttcagc ttgtagggat cgcggcgttc cacgcgggtg agcgaagcct
     1501 ttgccaacgc ggtttccaag cgtatgacgg tatcggcatt ctgcatggtg gtttcggggc
     1561 gatcgcccat cagctcgaaa atcttccgca cgtgctgggc gtaacgctcg cgggtctgtt
     1621 tcgatttgtc gtcagtcttg gtgtagtaat cccggtcggg gaggccgagc ccaccctgat
     1681 ccaattcggc gattatctct tcggagttgt ccgggtcttg cgttgaccct gccgcaaaga
     1741 gaatggatcg gccatagacg agctgcagcc gggcaattag cgggggcagc tcccgggtgc
     1801 tcttgagctt agcaatggct tcgagctgag attgaacggc ggaaatgcca cgcctttcta
     1861 ccttggcgtc gtccatacaa gcagcgtagt aatcaccgac ctcttgcgtc accgcatcac
     1921 ggtgattggt gtccgatgct gcctgctcga gaatgccgtg caggaagttg agattgtcct
     1981 ggtagagctt ggcgtaaacc gaccaggaag tctgatcgga tgggatggga ttcttgctct
     2041 gccatcctcc gcaggcgtag cgatagaagt ccacacaggg atcgactgac ttgtccatgg
     2101 aagtgacatc caagctcggg caatacggga gaaccggctg ctgtgagact tttgtcccgg
     2161 attggtcctg cgcggttgcc agcgatgcgg aaatgagaaa gagaagggat aagtaaccaa
     2221 gtcttggatt catattcacc ttcgacagcg atggttgaaa aaggcaatga gcgtagcaat
     2281 gatagtagag tgcgggttct cccaggcaag atttcctatt tgacttccaa atccgccggc
     2341 attgtacatc gactcgcttt ccttcgagct tctgacgtgc gtctctccag ttggaccacc
     2401 tcagccgaat gggtcgttcc ggaagcgaag cttccagaag atttggcgga ggatgccgag
     2461 ggtgagatcg gcgtcggtag cggagaggtg caggcggcgg acgaggcggc ggatcttttc
     2521 ctcggtgggg gtggcggagc gccttttcat ggagccgctg ggattcgatt tcaaatagcc
     2581 gctggagcag agggcgtcga gcaagagcgc ggtgaggcga tcgagatcgg cggcggtggc
     2641 ggcgatatgt ttctccggtt tcggcgcggc ttgggagtcg cgcgcgagtt cgtagagaca
     2701 gacggccacg gcctggccga ggttcatgga gcgatgctct tcgcgggtgg ggatgcgcag
     2761 gagccagtgg cagtggctga ggtcttcatt cgaaagacca cgcttttctg aaccgaagag
     2821 aagggcgacg cggctgccgg cgagatgttt gtggaggaga cgggcggctt gatcgaggcg
     2881 gcggaccggg tgctggggtt ggcggcgacc gacggcagtc gttccgacaa cgagggtgca
     2941 gtccgcgacg gcttcggcga cggtcttgaa ttcttcggcg caggcgagca gcggcgcggc
     3001 gccgacggcg gatcgcgcct cgcgaaatgc cagctcatag gggttcacca cacgaagatg
     3061 gaggaagcca aagttgctca tggcgcgggc cgccgcgccg atgttgaggg ggttgcgcgg
     3121 ggcgacaagg atgacacgca gacggtcgga ttcggcgtgg ggagacaagg gagacgagtt
     3181 tacagcagga gcgaagcaat gaagtaagga agtaaagagg taaagaggta aggaaaagcg
     3241 ggctaccggc ttaaaggcaa ggcgacgcct gcaagttacc ggcgaagcaa tctcggaacc
     3301 aggaacgcgg ccacgaggcc gatgccgctc aaaacggtcg gcactaaggc cgcgtagcgc
     3361 atccacgacg aaaaaccacg gtagcccgcg atcccacttt caagaagcaa gaggtacggc
     3421 gacgaagcca caaaaatcat gagaattaga gtaagtcgcg tgggaatgtg atccatcgtg
     3481 cgactgctcc aagaacccga gggaccaaag ataattgcag caaaggctgc aagacccaag
     3541 aagaacccta atcgatacgc acctctgttg ataagcgggc tcatgcacac caaagcgagc
     3601 cccaatccga tcgcgaccac tgcgccagcg atcaatttat agaggacgcc cctatccggc
     3661 ggacagtctt cagcatgttc cacctaagcc tcaatgtgcc atgccttaac tacgcaaagc
     3721 agatcctagc ttgcggaatt ctcaatttga tgcgtgaccg gttcaaatgt aaccgcagcc
     3781 ggggggagtg cggcttgcac gggcggcggg cggtaacata gactgtggcg ggtaaaagca
     3841 gaaagcctgc cagcctggcc ggaagttccc acctttgcat gggcctgttg gcatggctga
     3901 gtccaatcgc tccacgaccc agacctgcag gggtcatcat ccagcgtctt tccggcgatg
     3961 cgagcaaccg gggcgtcgca ctttggaccg tgcggcggcg gaatgcggga ctggtagggg
     4021 gatcagggtg gcaactccac tggaaacctg ggtcgaagaa gcggcgaagc tgacgaagcc
     4081 ggagcgcgtg gtgttttgcg acggttctga agcggagtac cagcggatga tcgaggagat
     4141 gctgcacgcg ggagattcat acacgctgaa cgagaagacc tatccaaatt gccacctgca
     4201 tcgtagcagt ccgaatgacg tggcgcggac cgagcatctt acgtacatct gctcgccgga
     4261 taaagaagat gtaggaccga caaacaactg gatggacccg ggtgcggcga aacataaggt
     4321 gggtgcgctg ctggatggag cgatgcgcgg gcggacgatg tatgtggtgc cgtacgtgat
     4381 gggaccggtg aactcgccga tcagcaaggt aggcgtggag gtaacggaca gtccgtacgt
     4441 ggtggcgagc atgcggatca tgtcgcggat gggcaaggta gcgatggagc ggctgggggc
     4501 gtcgagcgag tttgttcccg gggtgcattc gttgggcgac ctggacccta tgcggcggta
     4561 catcatgcac ttcccgcagg agaaattgat ctggagcgtg ggatcgggct acggcggaaa
     4621 cgcgctgcta gggaaaaaat gttttgcact gcggattgcc agcgtgatgg cgcgcgagca
     4681 gggctggatg gccgagcaca tgctgatcct ggggctggag tcgccgggag gcaaggtgac
     4741 gtacatggcc gcggcgtttc caagcgcttg cgggaaaacg aacctggcga tgatggtgtc
     4801 cgcgctggag agccagggct accgcgtttg gacgataggc gacgacattt cctggatgat
     4861 gatcggcgag gacggatatc tgcacgcgat caatccggaa gcaggatttt ttggcgtggc
     4921 cccgggcacg ggaatgaaaa ccaatcagaa cgtgatgggg gcgctgcgca agaacacgct
     4981 gtttacgaac gtggcaatga cttcggaccg ggagccgtgg tgggaaggaa ttggcagcga
     5041 agcaccggcg gggttgatca actggaaggg cgaggcgtgg gatccatcca agggagcggc
     5101 ggcgcatccg aattcgcgat acaccgtgcc ggcgcagcag tcgccgagca tttcaccgaa
     5161 gtgggaagcc ctggaaggag tgccgatttc ggcgtttata tttggcggcc gaagaacacg
     5221 agtagcgccg ctagtgtatg aatcatttga ctggcagcac ggggtgttcg tgggcgcgac
     5281 gatggcgagt gagactaccg cggcggcgac gggcgacgtg ggtatttcgc ggcgggatcc
     5341 catggccatg ctgcccttct gcggctacaa catgtcggat tattttgggc actggctgga
     5401 gatgggcaag aaaataccta taccaccgaa gatttttcac gtgaactggt tccgcaaggg
     5461 cgctgatgga aaattcctgt ggcccgggta cggcgaaaac gtgcgcgtgc tgaagtggat
     5521 tctggagcgc gtagaaggcc gcggggcagc gcaggaaaca ccgatcggct atgttccggc
     5581 aaagaaaggg ctgacactgg aaggaatgaa gatttcggat gaagcggtga acgaattgct
     5641 gcgggtgcat ccagaagatt gggaactaga gatggaagac tcaaagcagt tttttggaaa
     5701 attcggtgaa cgcctgccgc ggcaaattag cgaagaacat gaaaaactgg cgcgtcggtt
     5761 tcagcgcgtg gtcacggcct aggaaatcgc cataagcttc agtgaaagcc gccggcgcgg
     5821 cgcggcggcg agctgatggg aggcatatcc tcggcatcaa aggctgcggg ctcgcgctcg
     5881 aaatgaaagt cgggccagct ttccagtaat cggacacaac gattccagcg gagaatgctg
     5941 tcgtcgttgc ctgcggggcg gattttctcg gcttcggcaa aacaaagcat ggcttcctgc
     6001 actaacggca acagggtgtg gggagcgtag ccgacgagga gttgcacctt ggcgcggcgc
     6061 tcgtggagca gcccggtgta gtagaagcgc tcataggctt cgcgcaaagc ctgaaagatg
     6121 gactgcacct cggcatagcg atcgctcgcg gcgccgataa attggtcggt gatggcaaga
     6181 ccgagcatgc gccgcgcgag ctgatgcccg ggatcaacgg cgaggatgtc ccggcagatg
     6241 gattcggcct cttcgggctc gttgagataa cggtagagct cgactttggc gatggcttcg
     6301 gcgatgccgg ccttggaaat ggattttagt tggtgtccca tgggacctcc tttttcatgg
     6361 cccgttgctc gaacataact ctcgaagaat cctggccccg ctcaatcacg gtgcctgccg
     6421 gttcaccctg aaggctgtgc taccggccac gaatcttccg gaacagcatg gtcaacggat
     6481 cgtaaaactc atccaccacg cgctctttga gcgggatgat ggcgttgtcg gtgatgcgaa
     6541 tctcctcggg gcagacctcg gtgcaacatt tggtgatgtt gcaaagaccg aggccaagct
     6601 gatccctgag aagggcggag cggtcgccgc cgtcgagagg atgcatttcg agcccggcaa
     6661 cacggacgaa aaagcgcggg ccggcgaact gctccttctt gtcgtgttcg cgaagaacgt
     6721 gacacacgtc ctggcaaaga aaacattcaa tacacttgcg gaactcctgc acgcgatcca
     6781 cgtcgagctg cgagattttc catgcgacgc ccgggcgcgg ttcgaaagca gggattttgc
     6841 ggttgacacg atagttccag gaaacgtcgg tgacaaggtc tttgattctg ggaaaggatt
     6901 tcatgggctg gacggtgatg ggctggtcct gcgggagatc gtccatgcgc gttttgcagg
     6961 tcaggcgggg acggccgttg acttccgcgg agcaggagcc gcacttccct gctttgcaat
     7021 tccagcgaac ggccaggtcc ggagcgaggt ggccctgaat gtaatggagc gcatcaagga
     7081 caaccatgcc cggggcgacg ggaacgcggt agtcgatggt ttgtccaccg tttgaatcgc
     7141 cgcggaaaac gcgcaaggtt gcttcactca tgtttgaact ccgtgttgcg atcgctggat
     7201 ggcttcctcc cagcgcaaag gcagagccga cccggcaact ttccattgag tgacctttga
     7261 cggacccatg tgcggagtca tggccttcat ggcttcgccg tgcggaagct gtgcgacaaa
     7321 atccatcaag gctttttcat cctcccaaac ggagagcgtc caaaaattgc gactcagcac
     7381 cttggcgcgg agggagtatc caatcactcc gggagtgccg cgcaattgct tttggatctg
     7441 gaatgaaaaa cgcaagaaac taggaatcgc ccgatacttc ttgagaggca agtaggataa
     7501 gagcgcgagg tattcgcgat ttttttctgc gggcgcaagc gccttccact ggctatccat
     7561 ggtgatgggt ctcccagtca tacggtaaga caaatcaaga tccgtttcat gacttatcct
     7621 cggcaagaat ctgcttaagt tcgccgggca tttccggaat gggcgattcg ctgcgcttca
     7681 tggcgccgga ttcccggatg atgatgttgt gtttcttctc ccaggccgaa tcgagattcg
     7741 gatagtcgat gcggctgtgc gcgccgcgac tttccgtgcg caccaaagcg ctttgggtaa
     7801 ccgcttcgga aacaatgagc atggctttca ggtcgtacga gagatgccag ccgggattaa
     7861 agagacggga gccctcgacg ccggcctttg ccgcacgggc tctcagtttt tgcagctcga
     7921 caagcgccgt tttcaagtct tcttcggtgc gataaatccc gacgagatcg tgcatcactt
     7981 cctgcaaatc acgatgaacg gcataaggac tttccgcgcc ggtgttggaa aagggagcga
     8041 gtaattctct ttcagcttgc tcgatctgcg cgttgtcgag agaagcggcg gaggcgcgtt
     8101 tggcgtactc cgcggcgcca aggccggcgc ggcggccgaa gaccagtaga tcggaaagcg
     8161 agttgccacc gaggcggttc gaaccgtgca gaccggcggc agcctcgccc gcggcgaaga
     8221 gacccggcag agtggattgc gcggtttctg catccacgcg aatgccgccc atcatgtagt
     8281 ggcacgtggg accgacctcc atcggccctt tggtaatgtc gacgtcggcg agttccttga
     8341 actggtgata catgctggga agttttttct ttacgtactc ggcaggctga tgagaaatgt
     8401 caagatacgc gccgccgtgt tccgtgccgc ggccttcctt cacttcggtg tagatggagc
     8461 gggcaacgac gtcgcgcgtg gaaagttcca tgcgcttggg gtcgtatttc tccatgaatc
     8521 gctcgcccgc tttgttgcgc aggatgccgc cttccccgcg aactgcttcg gtgacgagaa
     8581 tgccctggac gccgggcggc caaaccattc cggtgggatg gaactggacg aattccatgt
     8641 ccatgagctc ggccccggct tcgtaagcga gggccaggcc gtcgcccgta tattcccagg
     8701 agttggaagt tatgcgccag gccttgccga tgccaccggt ggcgatgacg atggacttgg
     8761 ctttgaacac gatgaggcgg ccggtctcgc gccagtaagc gaaggcgccg acaacgcggc
     8821 caccgtcagt gagtaagcgt gttacggtgc attccatgta gacgtcgatg ccctgctgga
     8881 cgccgcgatc ctggagagtg cgaatcatct cgaggccggt gcggtcgcca acgtgacaga
     8941 ggcgtttgaa ggtgtggccg ccgaatgcgc gctgtagaat gtcgccattc tcggtgcggt
     9001 cgaagagtgc gccccactgt tcgagttcgc ggacgcggtc gggggattcc tgcgcgtgaa
     9061 gctgggccat acgccaattg ttgagaagct ttccgccgct catggtgtcg cgaaaatgcg
     9121 ttttccaatt gtcggcggcg tccacgttgg ccatggccgc ggcgatgccc ccttcggcca
     9181 tgacagtgtg ggctttaccg agaagcgatt tacagaccac cccgaccgag gcgccttgtg
     9241 cgagggcttc aatggccgca cgcaatcccg cgccgccggc gccgatgatg agaacgtcgt
     9301 gctcgcgggt ttcgtagttt gcggtcacag gagcctcagg tcacggatcg cgccgctggc
     9361 caccatccgc acgtaaaagt cagcgaaacc aaccgaaacg aggctgcacc aggcgaagag
     9421 catgtggagc tcgttcagaa atgtggagac gcgccaggca gcaaagcggg agcggccgaa
     9481 ggtcgcgcaa gagaagcaat cgagtttgcc gccgacaaga tgacgtagcg aatggcagga
     9541 aaacgtgtag agggaaagaa gagagacgtt gagactgaga acgattgtgc ccacgcccgc
     9601 gccgaagtgg ccgtcgaaga agaaagcgcg gaaggcgtcg tgccagagaa acgctataaa
     9661 gagaactgca aggtagaaaa agtagcgatg gacgttttga aggatgaagg gaaaggctgt
     9721 ttcgccgcga tagtcgcgac gcagaggttc accgacggcg caggcgggcg gatcgagcag
     9781 aaatgcccga tagagagcct tgcggtagta atagcaggtg gcgcgaaaac ccaaagggcc
     9841 aacgagaatg agtaaggctg gagaaaaagg ccaccagtga tgctgcgggt cgatgagcgg
     9901 cgagtaaaag ggcgataggt acggacccca agaatagtaa gcattttcga aggctcgaac
     9961 cgtggcatag atgctgaagc cacccatcat gatgatgatg gggatgatct cgatccacca
    10021 ggcatctcgg cgcacagtgc tgccgagacg cgattccggc aaaagggtcg gggagccaga
    10081 catcgggtca cctccgaagt ggctgtaggg tagtcaaaaa acagattttt tcaaatgaaa
    10141 ataatagcgg ggacggaagg atagagtgtt aaagaattgt ttcgatctcg gcgtcgagag
    10201 agacgtgagt ggcggggatt ccgttgatcg agaacttctc gccggcggct tcagagaatt
    10261 ggaagaaacg gctgatgtga tagtcctcgt catagccgga gaagaggcgt cccgtttcct
    10321 cgttcgtcaa accgaaggca gaaagcgcga ccggctcacc aaatttacca gcgtgagagc
    10381 ggtataaacg gaccagatcg cgcaggggca tgatgggagc gtggccaggg gcttcggcgc
    10441 tcatagggtt ctccggagca gagtgtactg ccgaggacaa aaagaaggaa tgaaattaac
    10501 gcaggatacg gagcgacgac gggatggagg gcaaccagaa tggcgggaac cgaaacttgg
    10561 tgcgggacgt gaggattggg taagatgctg agtgggacgt cagagaccca ggaggaaaga
    10621 gtatgagtat cgcggtggga cagacggcgc cggattttac actgttgaat caggaaaaga
    10681 aggaagtgaa gctttcggat ttcgcgggga gaaagaacgt tgtacttgtg tggtacccgc
    10741 tggactggag tccgacgtgc accaacgagc atgcctgctt cgtcaatgag atgaagtcgt
    10801 tcgaaacgct ggacgcggaa gtgcttggcg tcagtgtcga cagtgtgtgg tcgcacaagg
    10861 cgtatgcgga gaagatgggg atcaagtatt cgctgctggc ggattttcat ccgcggggag
    10921 cgatgagcga aaaatacggc gtatacctgg cggacaaagg gattacgggc cgggcgatcg
    10981 ccatcgtgaa caaaaccgga aaagtggcgt ggttcaagga ctacgacatt cctgtggtgc
    11041 cggatttaaa agaagttgca gccgcgctcg gacaagtgaa agcagcgacg gcgtaaagag
    11101 gaagtaagga aggaaaaagc gggccggtgg ctgtcggccc gctttttctt tgagcgagat
    11161 gaattgtgcg tggagtgaac tcgggctgat tcccagctta cttggcggcg taatcgttgc
    11221 ggacgacggt gccgcccttc atcacgaact tgacggattc aaggacgcgc acgtcggaag
    11281 ccgggtcacc gctgacagcg atcagatcgg cgtagtgacc ggtctccaac gccccgatct
    11341 tctcggccca gccaagcaga tccgcggcgt tgaccgtggc ggcctgaatg gcatcgagag
    11401 gcttcatgcc ccattcgacc atcttgc
//
LOCUS       DASYVO010000137         6979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_213605, whole genome shotgun sequence.
ACCESSION   DASYVO010000137 DASYVO010000000
VERSION     DASYVO010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6979
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213605"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3383)
                     /locus_tag="VGR03_04575"
     CDS             complement(<1..3383)
                     /locus_tag="VGR03_04575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV1993585.1"
                     /translation="MFVHLHCHSHYSFLRGVASPEEIVATAVEQKMPAVALTDTNGLY
                     AAVSFYQAAKRAGIKPIVGVVLDVEWSQDRRKIPSDKVGTFGRFARNDGNKPSATLVL
                     LAADMEGYNNLCQLVTLRHLGTTKLSQNMNSAETDGRPVTMQELAEHSRGIIALCPLP
                     ARSRDASPCSGAACCATTRATQANSDGHEQKLSATNHQSPNTSHFSQLKEIFGDRLYI
                     EVQHLSPGDGRELREAERLGRELGVPLVATNNVHFIRPEEHLHHRAVNAIRTGGLLTT
                     VAPPEITTGEAWFKPAAEMQKCFPDHPELLQATLEIAGCCDLQIELGKLIFPEFPVPD
                     GESAFSYLWKLSFDGARKRYKPLRPEVLSRLTHELEVIEKLHLAPYFLLVWDIVEEAR
                     RRGIPAVARGSAASSIVTYCLGISCVCPLRWGLYFERFLNEQRGDCPDIDIDICGARR
                     DELLDYVYERWGAEHVAMIGSFITMHARLAVREIAKVFGVPPGEVNRFTKRLPHRPVR
                     EILTAIRDLPECRDLPIDDEPWNTILQVALRLDDAPRHMGIHPCGTVISARPLTRLTP
                     LERATKGIVVTQYDMNAIEALGLIKMDLLGQRGFTTMSLALDNIEKKEIKERKEVEEI
                     REKNNVAPDGTTPCPKPREIDFAAIPENDPATCEVIAAGRTMGVFQIESPAMRGLLRT
                     MKARTLEEIAAALALIRPGASEYGSKELFLKRLRGQEPVLYAHPSLQNILGDTLGVCI
                     YQEQVMQIAQAVGSMSLQEADLVRRSAAKFSGQRERERLRGKFLKAAEQMGLTGAERE
                     ETWMMVEKFAGFGFCKAHAATYADISYRMTYLKTHHPAEFLAAMCSAGAGFYHVSAYV
                     EEAKRWGIEVRLPAVNQSRMEYTAEAGEDGKRALRVGLMQVRGLRVETITAILRTRGE
                     GGEFQSLEDFLRRVPVERDEIESLIKCGAFDGMNDEVCRMTRPEMLWRWNLLQVKKKN
                     GQPCAPRFRSGQGAVLAESAKTLFAEANARDAISVALAEMQTPEYTLEQKLRYEREIL
                     EVCVSGHPLDFLPRNGEAWSDELPGLRGKRVTLCGWVVTYRHVGTKNYRNMMFVTLED
                     QRGVYEVVLFPDAYDRYGGLVFETRTMRVTGRVEQDGQI"
     gene            complement(3485..5035)
                     /gene="dinB"
                     /locus_tag="VGR03_04580"
     CDS             complement(3485..5035)
                     /gene="dinB"
                     /locus_tag="VGR03_04580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV1993586.1"
                     /translation="MRPASLSGAAMDVCVENVFTAPAIEAINVIQATAEAENVEDSFD
                     LKRESQDKEAPRYRDAGQEAVAQSPSNDPRASASPASPAETPRTETAVEKNPWSAWAW
                     LGMCKNSSRKIPNTIPNIVHVDVDAFFASVEQVLNPKLRGKPVLVGRGCVASASYEAK
                     FRGVKTAMSFREALRICPKAIVVPGQYEHYADFAERVRRILETYTPAVETAALDDFYL
                     DFAGTERLYPDYEATLRRLQGEIRSRTGLSVSVGAARTKVVASIASRLERPRGFRMIA
                     PGEEEHFLTPLPVEKLHGIGHVHAGALAERGIATIGQLRQVPKPALQATFGEVIGQQI
                     WERARGLDGREVLLPSTPKSVSRETTIEGGTIDTEFLGGLIEYLSERIGSTLREYGKQ
                     ARTIGLRIRCVDHFSAHQTVRISKPTNDERELLAAAKDLFGKLFTRRVAVRLVGVNVI
                     NLETDKRQHELFDTNANRRWYLNRGLDSVRGRYGWNAVFYGNGLELREHYATKPNGLV
                     LSTPCLSR"
     gene            5326..5937
                     /locus_tag="VGR03_04585"
     CDS             5326..5937
                     /locus_tag="VGR03_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993587.1"
                     /translation="MAMRISDKTWRRIRRALLLLLGGVAVLAIAGWAERERNADEEYA
                     VYSAYLSEGLLNDAHDWSVGGPVQVVVRDTTKVGGNLRLRALYVLDGRVHFDQLHTST
                     RASYLVRNLFQTRILPRFVLPSRATVALASQSDYGSPDFQEKFPHNLGLIVLSGVGFN
                     PTRTQAVFYIDHFCGLCGGGRYVLMEKIDGSWRVRGEHYTWVS"
     gene            5985..6326
                     /locus_tag="VGR03_04590"
     CDS             5985..6326
                     /locus_tag="VGR03_04590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEV1993588.1"
                     /translation="MTQQASELLQKALSLSEEERAELAGSLIESLDATVDQAAEAAWN
                     QEIARRIEALDSGKAKTVPWEEVRSRISTKFFASRKARADFKAFDKLMGRRGGKRPRA
                     GDELPPRTRGE"
     gene            complement(6425..>6979)
                     /locus_tag="VGR03_04595"
     CDS             complement(6425..>6979)
                     /locus_tag="VGR03_04595"
                     /inference="COORDINATES: protein motif:HMM:NF039873.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993589.1"
                     /translation="HVGQARLHYLLDHTDDPVIPRRGFSTESNFRWFDSSPGAKGGFP
                     SMDLKVGYFQPITRPASIFLEGEGGTTFGSTSTGIPQFFLGGPVRLSAYGQNEFQGNQ
                     YYLFRAGYMHDLLTLPPFVGKKVYAVGSYEIGKMYGVTTGTEFPNDVAAGLLAETALG
                     PFFIGGSVGDSGHRKWFFQLGRVF"
BASE COUNT         1379 a         2097 c         1984 g         1519 t
ORIGIN      
        1 ttgatctgcc catcctgctc gacgcgcccg gtcacccgca tcgttcgtgt ctcaaacact
       61 aggccgccat agcggtcgta cgcatcgggg aaaagcacca cttcatacac gccgcgctgg
      121 tcctccagcg tgacgaacat catattgcgg taattttttg ttccgacatg acggtaagtc
      181 actacccagc cgcacagcgt cacgcgcttt ccgcgcaggc ccggcagttc atcgctccag
      241 gcttcgccgt tgcgcggcaa aaaatcgagc gggtggccgc tcacgcagac ttccagaatc
      301 tctcgttcgt agcgcagttt ctgttcgagg gtatattccg gcgtctgcat ttccgccaat
      361 gccacgctga tggcatcccg cgcgtttgct tctgcaaaca atgtttttgc actttccgcg
      421 agcacggccc cttgtcccga gcggaatcga ggggcgcatg gttgtccgtt tttctttttt
      481 acttgcagca ggttccagcg ccacagcatc tccggccgcg tcatcctgca cacttcatcg
      541 ttcattccat caaatgcacc gcacttgatc agcgattcga tctcatcccg ttccaccggc
      601 acgcggcgca aaaaatcttc gagtgattga aactcgccgc cttctccccg cgtccgcaga
      661 attgccgtga ttgtttcgac tcgcagacct cttacttgca tcaagccaac gcgcagcgca
      721 cgttttccat cctcgccggc ctcggccgtg tactccatcc gcgactgatt caccgctggc
      781 agccgcacct cgatgcccca gcgcttggct tcttccacat aggccgacac gtgatagaac
      841 cccgcgcccg cgctgcacat cgccgcgaga aactcggccg gatgatgcgt cttcaagtag
      901 gtcatgcggt aagaaatgtc cgcatacgtt gcggcatgcg ctttgcaaaa accaaacccc
      961 gcgaacttct ctaccatcat ccacgtctct tcgcgctcgg ctccggtcag gcccatctgc
     1021 tccgcggctt tcaggaattt gccgcgcagc cgctcgcgtt cgcgttgtcc agaaaattta
     1081 gccgccgacc gccgcaccag atcggcttct tgcagcgaca tgctcccgac tgcttgcgcg
     1141 atttgcatga cctgctcctg atagatgcac acacccagcg tgtcaccgag aatgttttgc
     1201 agagacggat gcgcgtacag aacaggttcc tgcccgcgca ggcgttttag aaacaattcc
     1261 ttcgagccat attccgacgc gcctgggcgg atcaacgcca gcgccgctgc gatttcttcg
     1321 agcgtccgcg ccttcatcgt gcgcaacaat ccgcgcattg ccggcgattc gatctgaaac
     1381 actcccatcg ttcgtcccgc tgcaatgacc tcgcacgtcg cgggatcgtt ttccggtatc
     1441 gcggcaaaat caatttctcg cggcttcggg cacggcgtcg ttccgtcggg cgcaacgttg
     1501 tttttttctc tgatctcctc tacttccttt ctctccttaa tttctttttt ctcgatgttg
     1561 tccaacgcga gcgacatggt tgtgaagccg cgctgtccaa gcagatccat cttgatcagc
     1621 cctagcgctt cgatggcgtt catgtcgtac tgcgtgacga cgatcccttt tgtcgcgcgc
     1681 tccagtggcg tcagtcgcgt caatggccgc gcggaaatca ccgtcccaca cgggtgaatc
     1741 cccatgtggc gcggtgcgtc gtcgagacgc aacgccactt gcaggatcgt gttccatggc
     1801 tcgtcgtcga tcggcaggtc gcggcattcc ggcaggtcgc gaatcgccgt tagaatttcg
     1861 cgcaccgggc ggtgcggcaa tcgcttcgtg aagcgattca cttcgcccgg cggcacgcca
     1921 aaaactttgg cgatttcgcg cacggccagc cgcgcgtgca tggtgataaa gctgccgatc
     1981 atggcgacat gttcggcgcc ccagcgctcg tagacgtaat cgagcagctc atcgcgccgc
     2041 gcaccacaaa tatcaatgtc gatgtccggg caatcgccgc gctgttcgtt cagaaaacgt
     2101 tcgaagtaaa ggccccagcg caacgggcag acgcacgaaa tgccgaggca atacgtgacg
     2161 atggaacttg ccgctgaccc gcgcgccacg gcaggaatgc cgcggcgccg cgcttcctcc
     2221 acgatgtccc agaccagcag gaaatacggc gccagatgca gtttttcgat gacttcaagt
     2281 tcgtgcgtca gccgcgagag cacttccgga cgcagcggct tgtaacgttt gcgagcgcca
     2341 tcgaagctca gcttccacaa atacgaaaac gccgattcgc cgtcgggcac ggggaactcc
     2401 ggaaagatca gcttgcccag ttcgatctgc aaatcgcagc agccggcaat ttccagcgtt
     2461 gcttgcagca actccggatg atcggggaaa catttttgca tttccgccgc cggcttgaac
     2521 catgcctcgc cggtggtgat ttccggtggt gccaccgtcg tcagcaatcc gcccgtgcgt
     2581 attgcgttca ccgcgcggtg atgcagatgc tcctccggcc ggataaaatg cacgttgttt
     2641 gtcgcaacta acggcacgcc cagttcgcgg ccgaggcgtt cggcttcgcg caattcgcgc
     2701 ccatctcccg gcgagagatg ttgcacttcg atatagaggc gatcgccgaa aatctccttg
     2761 agttgcgaaa agtgactggt gtttggtgac tggtgattcg tggctgagag tttttgctcg
     2821 tggccatcgc tatttgcttg agttgccctt gtagtggcgc agcatgctgc gccactacaa
     2881 gggcttgcat cgcgcgatct tgctggcaag ggacacagcg cgatgatgcc gcggctgtgt
     2941 tccgccaatt cctgcatggt cacgggccgt ccgtccgttt cagccgaatt catgttttgt
     3001 gacaattttg ttgtgcccaa atggcgcagc gtcacgagtt ggcaaagatt gttgtagcct
     3061 tccatgtccg ccgccaacaa aaccaatgtg gcgcttggct tatttccgtc attccgagcg
     3121 aagcgaccga aggttccgac cttgtcggaa ggaatctttc tccgatcttg tgaccattcg
     3181 acatccaaca ccacgccgac gataggcttg atgcccgctc ttttcgcggc ctggtaaaac
     3241 gagacggcgg cgtacagccc attcgtatcc gtcagcgcca cggccggcat tttctgctcc
     3301 accgctgtcg cgacgatttc ttccggcgac gccacgcctc gtagaaacga atagtggcta
     3361 tgacaatgca aatgtacgaa caagggagcc ccagcccgga aagggaagaa ggcatgcccg
     3421 tctttcggca ggcatgcttg tcaattcgaa tcaggtaggg gaagggaagg aaaaactcag
     3481 cgaactaccg cgataaacat ggtgtcgaga gaaccaggcc gttcggcttg gtggcgtaat
     3541 gttcgcgcag ctcaaggccg ttgccgtaga agacggcgtt ccagccgtac cggccgcgca
     3601 cggaatcgag gccgcgattc aggtaccagc ggcggttggc gttggtgtca aagagttcgt
     3661 gctggcgttt gtcggtttcg aggttgatca cgttgacgcc gaccagtcgg acggccacgc
     3721 ggcgcgtgaa caatttgccg aacagatctt ttgctgcggc gagcagttcg cgctcgtcgt
     3781 tggttggttt gctgatccgc accgtctggt gcgccgaaaa gtgatcgaca cagcggatgc
     3841 gcaggccgat ggtgcgcgcc tgcttgccgt attcgcgcag ggtcgagccg atgcgctcgc
     3901 tgagatattc gatcaggccg ccgaggaatt ccgtgtcgat ggtgccgcct tcgatagtcg
     3961 tttcgcggga aacggatttc ggcgtggagg gcagcagcac ttcgcggcca tcgaggccgc
     4021 gggcgcgctc ccagatttgc tggccgatga cttcgccgaa ggtggcctgc agcgcgggtt
     4081 tgggcacctg gcgcaattgc ccgatggtgg caatgccgcg ctcggccagc gctccggcat
     4141 gtacgtggcc gatgccgtga agcttttcca ccggtagtgg cgtcaggaaa tgttcttctt
     4201 cgccaggcgc gatcatacga aacccgcgcg ggcgctccag ccgtgaagcg atggaggcga
     4261 ccacttttgt gcgcgccgcg ccaaccgaaa cgctcaagcc cgtgcggctg cgtatttccc
     4321 cctgcaagcg tcgcagcgtg gcttcgtaat cgggatacag ccgctcggtg ccggcaaagt
     4381 cgaggtaaaa atcatccagc gcggcggttt ccaccgcggg tgtgtacgtt tcgaggatcc
     4441 gccgcacgcg ttccgcaaaa tcggcgtagt gctcgtattg cccgggcacg acgatggctt
     4501 tcgggcagat gcgcagcgct tcgcggaagc tcatcgccgt tttcacgccg cggaacttcg
     4561 cttcgtagct cgccgaagcc acacagccgc gccccaccag cacgggcttg ccgcgcagct
     4621 tgggattcag cacttgctcc accgacgcaa aaaacgcgtc cacatccacg tgcacgatgt
     4681 tgggaatagt gttgggaatt ttccggctgg aatttttgca cattcccagc caagcccagg
     4741 cggaccacgg atttttctcc accgccgttt ccgtccgtgg cgtttccgct ggactcgcag
     4801 gactggcgct tgccctggga tcgttcgaag gactctgagc aacagcttcc tgccccgcgt
     4861 ccctgtagcg cggagcttcc ttatcttgag actcgcgctt taggtcgaag gaatcctcga
     4921 cgttttccgc ttccgcggtt gcttggatta cattgatcgc ttcaatggcg ggggcagtga
     4981 atacgttttc gacacagacg tccatggcgg ctcctgaaag ggaagcggga cgcatatact
     5041 gtctcccgag cgaaggagcc gaatcagtcg gcttccggag tcgagggatc actctttcga
     5101 gacgacccct caccccgctt acggctcgga actttcaacg ggcggcggcc gtctcgtgag
     5161 agccttgacg gcaatggagc cggcgcgcca cccgtttccc gtttcctcta ccgccccgca
     5221 ctataggcga ataaaaagcg aaagtcaaga attattcaaa acttggaagg ggttggagtg
     5281 tccccccagc gagttcggga acttgtgttt caattgggga gtcgaatggc tatgcgaatt
     5341 agtgacaaga cgtggcggag gattcgtcgt gcccttctgt tgttgttggg gggtgtagct
     5401 gttctagcaa ttgccggttg ggccgaacga gagcggaacg cagatgaaga atacgctgtg
     5461 tattcggcgt atctctccga gggcctgtta aatgatgccc atgactggtc agtgggcggg
     5521 ccagtgcagg tggtggtcag ggacactacg aaagtaggcg gaaatctgag attgcgggca
     5581 ctctatgtgc tggatggccg cgtccacttt gaccagctac acacatcaac tcgcgccagt
     5641 tacctcgttc gcaacttgtt tcaaactcgg atacttccaa ggtttgtttt gccgagccgt
     5701 gccacagtag ccctggcttc acaatccgat tatggctcgc ccgattttca agagaaattt
     5761 ccacacaatc tgggactcat cgtcctctcc ggtgtaggtt tcaatcccac tcgcactcaa
     5821 gccgtatttt acatcgacca cttttgtgga ctgtgcggtg gtggacggta tgtgctgatg
     5881 gaaaagatcg atggctcctg gcgtgtgagg ggcgagcatt atacgtgggt ttcttgagct
     5941 agggtcgcca ctccaatcgg cggtctcaga ggtataatga ttttatgacg caacaagctt
     6001 ccgagttact tcagaaggcg ctctcactct ccgaagaaga acgcgccgag ctggctggct
     6061 cgctcatcga gagcttggac gccactgtcg accaagctgc cgaggcggcg tggaatcagg
     6121 agattgcccg ccgcatcgag gctctggact cgggtaaagc gaagaccgtc ccctgggaag
     6181 aagttcgatc aaggatttcc accaaattct tcgccagccg caaggcccgt gcggacttca
     6241 aggcttttga caagctcatg ggacgccgcg gcggcaagcg accgcgtgcc ggcgatgaac
     6301 tgccaccccg caccagaggc gaataaaaaa aggcgaaaaa acaggctcca atcgccaggc
     6361 tcagaggaaa aaagcagatc cctcgcttcg ctcgggatga cagtaaacgc ttatcagcaa
     6421 actattagaa cacgcggccc agttggaaaa accacttgcg atggccggaa tcgccgacgc
     6481 tgccgccgat gaagaagggg cccagcgccg tctcggcgag caaacccgcg gcgacatcgt
     6541 tggggaactc ggtgccggtg gtcacgccat acatttttcc gatttcgtag gagccgacgg
     6601 cgtagacctt ctttccgaca aacggcggca gcgtgagcag gtcgtgcatg tatccggcgc
     6661 ggaataaata atactgattg ccctgaaact cattctgacc gtaagcgcta agccgcaccg
     6721 gcccgcccag gaagaactga gggattcccg tgctcgttga gccaaacgtc gtgccgcctt
     6781 cgccctccag aaaaatcgac gccggtcgtg taatgggctg gaaatatccc accttcaaat
     6841 ccatggaagg gaagccaccc tttgcccctg ggctgctatc gaaccatctg aaattcgatt
     6901 ccgtactgaa accacggcgc ggaatcacgg gatcgtccgt atggtccaga aggtaatgta
     6961 gacgggcctg ccccacgtg
//
LOCUS       DASYVO010000138         4208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_160003, whole genome shotgun sequence.
ACCESSION   DASYVO010000138 DASYVO010000000
VERSION     DASYVO010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160003"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VGR03_04600"
     CDS             complement(<1..757)
                     /locus_tag="VGR03_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993590.1"
                     /translation="MSVKQPSLRGLTACLVVLAIFLAVIFGGASTLIAAAKRQVTGEQ
                     DKDKKEEKKDEKKDEKKDEKKGLPLKSDRKIEFTTDEGTWLSLDVSPDGKTIVFELVG
                     DIYTLPIEGGQAKLIAGGMAFDSQPKFSPDGKWIAFLSDREGSENIWIMHPDGTGPKQ
                     VSKDPNSEFTSPSWAPEGNYIFVSKAPFGIGANEIWMYHVDGGSGVQITKSKPAPTTR
                     RQDRHNAMGATASADGKYLYYAVRQGPFSYNVQF"
     gene            complement(912..>4208)
                     /locus_tag="VGR03_04605"
     CDS             complement(912..>4208)
                     /locus_tag="VGR03_04605"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993591.1"
                     /translation="LRTILACAAALGALAMSAQAAPGDQPNPTDKFKNLEFREIGPAT
                     MGGRIDDFAVVESTPNIVYVGTASGGVWKTTNNGTTWEPIFDKESVSTIGDIAIAPSD
                     PSVVWVGTGEPNNRQSSSWGDGAYKSLDGGKTWKKMGLELTRHIGRIVIHPKNPDVVY
                     VAALGHLWGANPERGVYKTADGGKTWSQVLKINDDTGVSDLAMDPQSPDTLYAAGYER
                     RRTPYGFNGGGPDSAIYKTTDGGATWKKLTKGLPYENGGETGRIGLDVYRKDPNIVYA
                     IVQHEKGGTYRSEDKGETWKKMGDTNPRPSYYSQIRIDPNNDLRIWELGAPMFYSEDG
                     GKTFSTQRVRGIHGDYHAMWIDPVDSNHMLTGSDGGIHWSYDAGKTWDFVNTIAIGQF
                     YEVALDNEKPFHICGGLQDNGSWCGPSQTLTRDGIVNEDWQVIHGGDGFYAAIDNVEP
                     WIVYTESQDGYIDRRDLRTGQQRSIKPEAKAGEPHYRFQWNSPVAVSAHLHTTVYYGG
                     NYLFESTDRGDNWTRLGGELTTGVDRNKLQIFGKTPDKNTLSRHDGVQEYPTITTLSE
                     SPLTPNVLWVGTDDGNVQVTRDGGKSWKNVALKVPGVPKGTYVSRVVASKTAEGAALL
                     AFDGHRGDDNSVYVFATSDFGENWKAIRNGIPDSAGSVHVVREHPRNTNLLFAGTEFG
                     LWVSWDRGGNWTALKNNFPTVPVDDIEIQARENDLVLATHGRSIWVFDDLTPIEKMDA
                     NVAASPLTFFPPRTATTWHLRNRRWSGGQKAFTAKNPPYGAILSYYLKDGVQPEAPKK
                     DKDEKDKKHAAEEKPKTEAKGDAAEKKEGKVEITVLDKDGKVLRELDGPGAAGVNRTN
                     WDLRSDASAEPTSQQLEAIAAGYGFGPRGPFVEPGEYTVKIKAGDKEASQKVMVEEDT
                     RIVISAADRSARREMIDQLYPMAKSTDKDRRTIEGIQTALKTARELWKKDAGKPNTTK
                     ISDDIVKAADELQKRVDAVAEKFIRAREGLGNAGPPFEWKPDPLPIQAQDLLDDLDGF
                     AAAPSGHQKEKLAELTPLVNDASAQVKKIAEEELPALNKKMNDAGIPHIVPAPPQPRG
                     GRGGEEESDQ"
BASE COUNT          690 a         1372 c         1103 g         1043 t
ORIGIN      
        1 gaaactgcac attgtaagaa aacggcccct ggcgcaccgc gtagtagaga tatttcccgt
       61 ccgcggaagc tgtcgcgccc atcgcattgt gccgatcctg ccgccgggtt gtgggcgccg
      121 gcttcgactt cgtaatttgc acgcccgatc cgccatccac gtggtacatc cagatttcgt
      181 tcgccccaat cccgaagggc gctttcgaaa cgaaaatgta attcccctcc ggggcccagc
      241 tcggcgaagt gaattcgcta ttcgggtcct tggagacctg cttcggacca gtgccgtccg
      301 gatgcatgat ccagatgttc tcgctgcctt cgcggtcgga gaggaacgcg atccattttc
      361 cgtccggcga aaactttggc tggctgtcga aggccattcc accggcgatc agttttgctt
      421 gcccgccttc gatggggagc gtgtagatat ctccgaccag ttcgaagaca attgtttttc
      481 cgtccggtga aacgtccagc gagagccacg tgccctcatc ggtggtgaat tcgatcttgc
      541 ggtcagactt cagaggcaat cctttttttt cgtctttctt ctcatccttc ttctcgtcct
      601 tcttttcttc tttcttgtcc ttgtcctgct ccccagtaac ctggcgcttc gcagctgcga
      661 ttagcgtgct tgcgcctccg aaaatgaccg cgaggaatat tgctaagaca acaaggcacg
      721 cggtcagacc gcgcagactg ggctgcttca cactcatgct gatttcctcc gcgtcatcta
      781 tcgaaagtta aacaggattt cagagatcgg cattccggtg gttatgggcc cgccgattga
      841 cacacccttt tctggccgtt agacgcaagc cgcgtggaag cgtctctctg atacaaactc
      901 ccgagcccgc gctactgatc cgactcctct tcgccgccac gtccgccgcg cggttgcggc
      961 ggcgcaggga cgatgtgggg aatgcccgcg tcattcatct tcttgttcag cgcgggcagt
     1021 tcctcctcgg cgattttctt tacctgcgcc gaagcgtcgt tgaccagcgg cgtcagctct
     1081 gcgagctttt ctttttggtg tccgcttggt gcggcagcga aaccgtccaa atcatccagg
     1141 aggtcctgtg cttgaattgg cagcgggtcg ggcttccatt cgaacggagg ccccgcattc
     1201 cccagccctt ctcgggcgcg gatgaacttc tcggcgacgg catccactct cttctgcagc
     1261 tcgtcggcgg ctttgacgat atcgtcagaa atctttgttg tgttgggctt gcccgcatct
     1321 ttcttccaca gctctcgcgc ggtcttcagc gcggtctgta tgccttcgat tgttctccgg
     1381 tccttatccg tggacttggc catgggataa agttggtcga tcatttcgcg ccgcgccgac
     1441 cggtccgcgg ccgaaatcac gatgcgcgta tcttcctcga ccattacttt ttgcgaggct
     1501 tccttgtctc ctgccttgat cttgacggtg tattccccgg gctcgacgaa cggcccgcgc
     1561 ggcccgaatc catatcccgc ggcgatcgct tccagttgtt gggatgtcgg ctccgccgac
     1621 gcgtccgaac gcaaatccca gtttgtgcgg ttcacgcccg ccgcacccgg cccgtccaac
     1681 tctcgaagca ctttcccgtc tttgtcgagc acagtgattt ctactttacc ctccttcttt
     1741 tccgctgcgt cgcccttggc ctcggtctta ggcttctcct cggccgcgtg tttcttgtcc
     1801 ttttcgtctt tgtctttctt tggcgcttcc ggttgcacgc catccttcag gtagtagctc
     1861 aaaatcgcgc cgtagggcgg attctttgcg gtaaacgctt tttgccctcc gctccaacga
     1921 cggttgcgca ggtgccaggt cgtcgccgtg cgcggtggga agaatgtcag gggcgatgcc
     1981 gccacgttcg catccatttt ctcgatcggt gtcagatcgt cgaataccca gatcgatcgc
     2041 ccgtgcgttg ccagcaccag gtcgttctcg cgcgcctgga tttcgatgtc gtccaccggc
     2101 accgtcggga aattgttttt caacgctgtc cagtttccgc cacgatccca cgatacccac
     2161 agcccgaact ccgttcctgc aaacaagagg ttggtgttcc gcggatgctc gcgcaccacg
     2221 tgcacgcttc cggcggaatc aggaatcccg ttgcggatgg ctttccagtt ctcgccaaaa
     2281 tcgctggtgg caaaaacata gacgctgtta tcgtccccgc gatgcccgtc gaacgccagc
     2341 agcgccgcgc cttcagcggt tttcgacgcc accacgcggc tcacataagt ccccttgggc
     2401 actcccggga ccttcaacgc cacgtttttc catgactttc caccgtcgcg cgtcacctgc
     2461 acgttgccgt catcggtgcc tacccacaga acatttggag ttagcggcga ttcgctgagt
     2521 gttgtgatgg tgggatattc ctgcacgccg tcgtgccgcg acagggtgtt cttgtccggt
     2581 gtctttccaa aaatctgcag cttgttgcgg tccacccccg tcgtcagttc tccgccaagt
     2641 ctcgtccagt tgtccccgcg gtccgtggac tcaaagagat agttcccgcc gtaataaacc
     2701 gtcgtgtgca ggtgcgcgga aaccgccacc ggcgaattcc actggaagcg gtagtgcggc
     2761 tctcctgctt tcgcctccgg cttgatcgat cgctgctgcc cggtgcgcag gtcgcggcga
     2821 tcgatatagc cgtcctgcga ctcggtgtaa acgatccatg gctccacatt gtcgattgcc
     2881 gcataaaaac catcgccgcc gtgaatcact tgccagtctt cattcacaat cccgtcgcgc
     2941 gtcagcgtct ggctcggccc gcaccagctc ccgttgtcct gcaagccgcc gcagatgtga
     3001 aacggctttt cgttgtcgag cgccacttcg tagaattgtc cgatcgcgat ggtgttgacg
     3061 aagtcccagg tctttccggc gtcatagctc caatgaatcc cgccgtccga gcccgtgagc
     3121 atgtgatttg aatccacagg gtcgatccac atggcgtgat agtccccgtg aatccctctc
     3181 acgcgctgcg ttgaaaacgt ctttcctccg tcctcggaat aaaacatcgg cgcgccaagc
     3241 tcccagatcc ggagatcgtt gttggggtcg atccgaatct ggctgtagta cgacggccgc
     3301 ggatttgtat cccccatctt cttccaagtc tcgcctttgt cttcgctgcg ataagtcccg
     3361 cccttctcgt gctgaacgat cgcgtagacg atgttcggat ccttgcggta aacgtccaac
     3421 ccaatgcggc cggtctctcc gccgttctcg tagggcagtc ctttcgtcaa ttttttccac
     3481 gttgcgccgc catccgtcgt cttgtaaatc gcgctatctg gtccgccgcc attaaatcca
     3541 tagggcgtgc gccgccgctc ataccccgcg gcatacaaag tgtccggact ctgcgggtcc
     3601 attgcgaggt cgctcacccc ggtgtcatcg ttgatcttca gcacttgcga ccaggtcttc
     3661 ccgccatccg ccgtcttgta cactccgcgc tccgggttcg ctccccacaa atgtcccaga
     3721 gccgccacat acaccacgtc cggattcttc gggtgaatca cgattcgtcc gatgtgccgc
     3781 gtcaactcca gccccatctt cttccaggtc ttcccgccgt cgagcgactt gtacgctcca
     3841 tcgccccagg aggagctttg ccggttgttc ggctcgcccg ttccgaccca caccactgac
     3901 gggtccgacg gagcaatcgc gatgtcgcca atcgtcgaca cactctcctt atcgaatatc
     3961 ggctcccagg tcgtgccatt gttggttgtt ttccaaacgc ctcccgaggc ggtgcccacg
     4021 tacacgatgt tcggagtgct ctccaccact gcgaaatcgt cgatgcgtcc gcccatcgtg
     4081 gcggggccaa tctcgcggaa ttccagattt ttgaatttat cggtgggatt cggctggtcg
     4141 ccgggcgccg cctgcgcact catcgccaac gcgccaagag ccgccgcgca agccagaatc
     4201 gtccgcag
//
LOCUS       DASYVO010000139         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_258014, whole genome shotgun sequence.
ACCESSION   DASYVO010000139 DASYVO010000000
VERSION     DASYVO010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258014"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="VGR03_04610"
     CDS             <1..1045
                     /locus_tag="VGR03_04610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1993592.1"
                     /translation="MPAGLLVVNATGIISSSNPAAEQVLGIRGLAFRRYSEALGASPD
                     LTKLIAECLSTGKIFRREEVEHLPPAGDTRHLGVTISPIRRDEGKISGAICLLTDLTE
                     LAVLQQRMQLKENLAALGELSAGIAHEFKNALATISGYAQMIRSESSEAEALDYAERI
                     LEQTRNITHVVTEFLKYARPLEIPDERVALQTMAERVVEEVGEALPQVKIRWEGTFGD
                     AGGDEGLLRQALLNLARNAAEACALTVGGGQVTVRGEIVHGEEAGFQRISVTDNGPGI
                     PANVLPKLFRPFFTTKAKGTGLGLAVVQKIIVQHGGQVEVRNRPEGGAEFIVTLPMPR
                     SVPEAVESRQTSI"
     gene            1089..1685
                     /locus_tag="VGR03_04615"
     CDS             1089..1685
                     /locus_tag="VGR03_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993593.1"
                     /translation="MRVRFWMKAFVIVPALCLAGLPAAAQQQGSSQQQTGDPVADAAR
                     KAREAKKDATKPKKVYTDDDLKKSEPAPDAASGSAPGNASGTAPATTVQNTGAATKAE
                     DPNGETAWRKRFKDHRDKIAKAEKELDILGREAQKAQVEYYPDPQKAMTQQNSRADIN
                     AINAKIDGKKKELDQLRQGLDDLEDQLRKSGGDPGWAR"
     gene            1831..>2340
                     /locus_tag="VGR03_04620"
     CDS             1831..>2340
                     /locus_tag="VGR03_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV1993594.1"
                     /translation="MTAAGFVVETKEMEPLLLVEDKTELRTMLRKALERAGYTVDEAP
                     DGAAAIQKVRARRYLMVITDLKMPGASGLDVLRETKQADAAMPVLLLTAYGSVEDAVA
                     AMKEGAFDFLQKPVDLEHLKLLVQRAAQQQELLRENLLLREEYSTRYGFPRIVGEHAS
                     IREVSQQIQK"
BASE COUNT          560 a          582 c          804 g          394 t
ORIGIN      
        1 catgccggcg ggtctgctgg tggtgaatgc cacgggaatt atctccagct cgaatccggc
       61 ggcggagcaa gttctgggaa ttcgcgggct ggcattccgg cgatacagcg aggcgctggg
      121 agcatcccca gacttgacga aactgatcgc ggagtgcctt tcgaccggga aaattttccg
      181 gcgcgaagag gtggagcatc tcccgcccgc tggagacacg cggcacctgg gcgtgaccat
      241 ctcgccgatc cggcgcgacg agggaaaaat cagcggggca atctgcctgc tgacggattt
      301 gacggaactt gccgtgctac agcaacggat gcaactgaag gaaaacctgg cggcgctggg
      361 cgaactgtcc gcaggaatcg cgcacgagtt caagaacgcg ctggcgacga tttcgggtta
      421 cgcgcagatg atccggtcgg agtcctccga ggcggaggca ttggactacg ccgaacgcat
      481 cctggagcag acgcgaaaca tcacccacgt agtgacggag ttcttgaagt atgcgcggcc
      541 gctggaaatt ccagacgagc gagtggcgct gcaaacgatg gccgagcggg tggttgagga
      601 agtgggggag gccttgccac aggtaaagat tcgatgggaa gggacattcg gggatgccgg
      661 gggagacgaa gggctgctga ggcaagcgct gctgaacctg gcgcgaaacg cggcggaagc
      721 gtgcgcgcta acggttggcg gagggcaggt gaccgttcgc ggcgaaattg ttcacggcga
      781 agaggcgggc ttccagcgga tcagcgttac cgataatggc ccgggaattc ccgcgaatgt
      841 attgccgaag ctattccggc cgtttttcac gacgaaagcg aaagggacgg gactgggact
      901 ggcggtagtg cagaagatta tcgtgcaaca cggcggacaa gtggaggtgc ggaaccggcc
      961 cgagggaggg gcggagttta tcgttacgct acccatgccg cgcagcgtgc cagaagcagt
     1021 agaatcaagg cagactagca tctaagagat tgtcggggtc ttccagctgc tccgggaggt
     1081 aagacgatat gcgagtgaga ttctggatga aggctttcgt aattgttccg gcgctgtgct
     1141 tggccgggct tccggcagcc gcgcaacagc agggttcttc ccaacaacag acgggcgatc
     1201 ccgtggcaga tgccgcgaga aaggcgcgcg aggcgaagaa ggacgcgacg aagcccaaaa
     1261 aagtctatac ggacgatgat ttgaagaaga gcgaacctgc gccggacgcg gcgtctggct
     1321 cggcccccgg aaatgccagc ggcacggcgc ccgcgacgac ggtgcaaaat acgggagccg
     1381 ccaccaaggc cgaagatccg aatggcgaga cggcctggag gaagcggttt aaggatcatc
     1441 gcgacaagat tgcgaaggcc gagaaggaac tcgatatttt gggacgcgag gcacagaaag
     1501 cacaagtgga atactaccct gacccgcaga aagccatgac gcaacagaac tcgcgcgctg
     1561 atatcaacgc cattaatgcg aaaattgacg gcaagaagaa ggaactcgat caactgcggc
     1621 aggggctgga tgacctcgaa gatcaactga ggaagtcggg cggcgatccg ggatgggcgc
     1681 ggtagaagag acgaagccaa aagttgtaag tttttagttg ttagaaaaat ccttcaattc
     1741 caagagtaag aactgcattg aggcgtggca ccggcctgag ggtgcggaag agctgcttct
     1801 cttctctgca cacgtgtctg tggttgttcc atgacggcag caggattcgt ggtagaaacg
     1861 aaagagatgg aacctctcct cctggttgaa gacaagaccg aattgcgcac catgctgcgc
     1921 aaggcactgg agcgcgcggg ttacaccgtg gacgaagcgc cggacggcgc cgcggcaatt
     1981 caaaaggtgc gcgcgcgaag atatctgatg gtgatcaccg atttgaagat gccgggggct
     2041 tcggggctgg acgttttgcg cgagaccaag caggcggatg cagcgatgcc ggtgctgctg
     2101 ctgacggcgt acgggtcggt ggaagatgcc gtagccgcga tgaaggaagg cgcattcgat
     2161 tttctgcaga agcctgtgga cctggaacat ttgaagctgc tggtgcagcg ggcggcacag
     2221 cagcaggagt tattacggga aaatctgcta ctgcgtgaag aatactccac acgctacgga
     2281 ttcccgcgga tcgtgggcga gcatgcctcg atccgggaag tgagccagca gattcagaaa
//
LOCUS       DASYVO010000140         8701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_58594, whole genome shotgun sequence.
ACCESSION   DASYVO010000140 DASYVO010000000
VERSION     DASYVO010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8701
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58594"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VGR03_04625"
     CDS             complement(<1..317)
                     /locus_tag="VGR03_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEV1993595.1"
                     /translation="MSCSRMEKKILGYVDGRLKEGERLEMEKHLSTCAACQLRVNEFR
                     AVSGLLDELPMIEPSAAFDIRLQARVAAEPAKQSWWAWFAPSPRVAFAASMLVLATVW
                     VGS"
     gene            complement(344..943)
                     /locus_tag="VGR03_04630"
     CDS             complement(344..943)
                     /locus_tag="VGR03_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1993596.1"
                     /translation="MARSDVQLMLDVKAGDEQSFALLLHRYRSPLVNFLYRMVRNREQ
                     AEDLAQEVFIRVYRARADYVPSAKFTTWLFRIATNLALNSVRDNRHQRMEISLDAPVT
                     IDAEDGDEKTLDVAEKRPNIEQHLVEEARRKMIRHAIDKLPEKQRAAVLLHKYQELDY
                     GEISKILECSESALKSLLFRAYETLRVELAPLVAQQARQ"
     gene            complement(1081..1824)
                     /locus_tag="VGR03_04635"
     CDS             complement(1081..1824)
                     /locus_tag="VGR03_04635"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEV1993597.1"
                     /translation="MGHDLAFRTALAAGLGLVAAAGNLIGGYFVVHKDWPRKFLQYFL
                     ALGAGYMLAVAFVEVIPESVRLSGENALLYVLIGYFLVHLFEHTLAPHFHFGEETHCE
                     EVSHHHARTSVLVGMTIHTFFDGVAIAAGFLVSTWLGAVMFFAVFLHKMPEGFTVASV
                     VLASGQGKRNAILAAGLLGVATVAGVLLTSSLQGQLKYALPLSGGVTLYVAATDLLPE
                     VNREPNWRMALLVFVGVASLLIMRHLLHV"
     gene            complement(1995..3065)
                     /gene="ychF"
                     /locus_tag="VGR03_04640"
     CDS             complement(1995..3065)
                     /gene="ychF"
                     /locus_tag="VGR03_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV1993598.1"
                     /translation="MQTGIIGLPQVGKTTLFRILTKAHVEGKAGASATHVGVAKVPEP
                     RLVELAKLYTPKKITYATVNYVDLGGMQKERMRDALAQLREVDVIAHVIRVFDDPSVP
                     HSEGSIDPLRDATNLDLELMLSDHDQITRRLERVDKDLKKKRDPILEIEKGALEKCKA
                     HVEAEKALRELELTPEERKSIGGFLFLSARPVLYVLNLGDDEADKLDTAVERHKLSAL
                     QGRPNAAVIAICGRLEAELSEMDEKEAAELLASYGLKEPGLNRLIRATYDLLGLIQFF
                     TAGEPEVRAWTIRKGATAVKAAGAIHSDIERGFIRAEVVRWNELLTAGSLAAAREKAQ
                     VRLEGKEYIVQEGDVILFRHSG"
     gene            3250..3666
                     /locus_tag="VGR03_04645"
     CDS             3250..3666
                     /locus_tag="VGR03_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993599.1"
                     /translation="MKESIRSLRLYFILSALASLWVCVVTFTPVLRASLSAVVIVAAV
                     FGIAFLAMSLAFLYVGFFLRRLLKDSSHRIVILIYVGMAWVVISFLANLFGGIQTQEI
                     VIDVLSMLLGLYLLKNIRRLAVEAQDPAADALPSTD"
     gene            3744..4472
                     /locus_tag="VGR03_04650"
     CDS             3744..4472
                     /locus_tag="VGR03_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993600.1"
                     /translation="MTATSQVLAFDLAEATKHLADRDECLKRLIEEAVPFQIDIADAQ
                     SPYEALLESIAYQSISGKAAATIYGRIKALSATGRAPTPQEMLKLRKPVLRKAGLSGA
                     KVLAMKDLAQKTIDGVVPNLEQAEKLSDDELVERLVSVRGIGAWTVEMFLIFRLGRPD
                     VLPIHDLGVKKGWSVTYGKKHMPKPKQLLAFGERWRPYRTVASWYMWRAFERAGYAAT
                     NKIRPAKVRRRKPANVTRRKRKPL"
     gene            4897..5586
                     /locus_tag="VGR03_04655"
     CDS             4897..5586
                     /locus_tag="VGR03_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993601.1"
                     /translation="MKALAEADGDVFLPVAEPEGPAHYVLIGMEPSLGWWAHSADEAR
                     SKIEAGYRNFLFSVEDFILHFCIRRYLCGPEEQYHITDLSKGAMLVDRAGLARVERYD
                     RWYGLLQEEIDLVATPNAGIAAVGRDVALHLERRRFARPFTRVVHYSSQAGRARSAGI
                     IGHEESFKGFIGSVSLDDLVTTAGHVLGAARVPAEIRDETLSRLARSQLTNSRQKLIF
                     NYKLAFELMRA"
     gene            complement(5783..6136)
                     /locus_tag="VGR03_04660"
     CDS             complement(5783..6136)
                     /locus_tag="VGR03_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993602.1"
                     /translation="MVGYGLWWMHMIGVVCGIAILLARLLRTRTIASLTTPAALCVVL
                     MILLTVVSQHAVSPKMAVLRVQMGSIQATPADSPLLAEFGRLHRISVSLESGVLLAGF
                     AAMYIMVRELASAGR"
     gene            complement(6309..6385)
                     /locus_tag="VGR03_04665"
     tRNA            complement(6309..6385)
                     /locus_tag="VGR03_04665"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6349..6351),aa:Val,seq:cac)
     gene            6454..7401
                     /locus_tag="VGR03_04670"
     CDS             6454..7401
                     /locus_tag="VGR03_04670"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV1993603.1"
                     /translation="MPTATSQVVSQLQRSIARAIYGKEDAIQLALITLLARGHLLIED
                     VPGVGKTTLAQALAKSFHCSFQRIQFTSDLLPSDVLGVSVYNPESREFEFRSGPIFAN
                     VVLADEINRTTPRTQSALLEAMNEAQVTVDGKTLPLPQPFLVIATQNPVEHHGTYPLP
                     ESQLDRFLMRIKMGYPSHETEKEILRKRVGDNPLDSLDPVADVSDVMAMQNAVAHVKV
                     DSSLHDYALEIVNRTRKSEQLALGVSPRGTLMLQRAAQARAYLDGRDYCLPDDFKQLA
                     VSVFSHRVVASSRHASLQKKSETTDSVLRDIVESVPVPL"
     gene            7562..>8701
                     /locus_tag="VGR03_04675"
     CDS             7562..>8701
                     /locus_tag="VGR03_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEV1993604.1"
                     /translation="MSAGKSTIAQLWAKADRPAVRAFLLSITALTAALLLALYSGAAA
                     EIGQPALATTSALAALAIAGWVGVILVPILAKRTPLRWIGYKMEYRITLEGWIYLGGI
                     ILVALAALNTGNNLLFLILASLIAIVLMSGILSSITLSGIGMRLVLPEHIFAGSPVRA
                     MVELENEKLTLPSFSLRVEAAKTKQAPPAAMLETPVYFPYLPKHDRVQQTIPVTFPRR
                     GVYRQEAFRVVTRFPFGFLRKARQMNLKTEALVYPSVEPTAEYLEILPGLEGALESLT
                     KGRGQDLYALRDYMPTDSARHVHWKASARSGSLMVREFTREDDCRILLVLDPHLSEEE
                     SSTLKPGQCATNERFERAVTLCASLAWHFYENNSLLKFRSAGVETP"
BASE COUNT         1814 a         2982 c         2035 g         1870 t
ORIGIN      
        1 tacgaaccaa cccacaccgt cgccagcacc agcatcgacg ccgcaaacgc cactcgcggc
       61 gacggcgcga accacgccca ccaactctgc ttcgccggct ccgccgccac gcgcgcctgc
      121 aggcgaatat caaatgccgc agacggctcg atcatcggca gttcgtccag caaaccgctc
      181 accgcgcgaa attcgttcac gcgcaactgg cacgccgcgc acgtcgagag atgcttctcc
      241 atctccagcc gttcgccttc cttcaggcgc ccgtccacgt atcccaaaat cttcttttcc
      301 atccggctac aactcatgta gtccttccct tcctggccca cttctactgc ctcgcctgct
      361 gcgccacgag gggggctagt tccactcgca gcgtttcgta cgcccgaaac agcagcgatt
      421 tcagcgcgct ctccgagcat tccaaaatct tcgaaatctc tccgtaatcc agctcctgat
      481 acttgtgcag gagcaccgcc gcacgctgtt tctccgggag cttgtcgatc gcatgccgga
      541 tcatcttgcg ccgcgcttcc tccaccaggt gctgctcgat gttcgggcgc ttctccgcga
      601 catccagcgt cttctcgtcg ccgtcttccg catcaatcgt caccggcgca tccagtgaga
      661 tttccatccg ctgatgacgg ttgtcccgca ccgaattcaa cgccagattt gtcgcgatcc
      721 ggaagagcca ggtggtgaac ttcgcgctcg gcacgtaatc tgcccgcgcg cggtacactc
      781 tgatgaatac ttcctgtgcc agatcttccg cctgttcccg gttccgcacc atgcggtata
      841 ggaaattgac cagcggactc cgatagcgat ggagcagaag cgcgaacgac tgttcgtcgc
      901 ccgccttcac gtccagcatc agttggacgt ccgacctggc catagccgcc acactgtatc
      961 aaaccctgct tgtctcagaa agttgcttgt gatttcttgc cagaaggaac tctaagctat
     1021 tgtaacgccg ggctttagcc cggcgtttac cttggttcgg aaaacgcccc gaaattgccc
     1081 tcaaacatgc aataagtgcc gcataatcaa caaactagca actcccacaa acaccagcag
     1141 cgccatccgc caattcggct cccggttcac ctccggcagc aggtccgtcg ccgccacata
     1201 cagcgtcacc cctccagaaa gcggtagcgc gtacttcaat tgcccctgca acgaactcgt
     1261 tagcaatacg cccgccaccg tcgccactcc caataacccc gctgccagaa tcgcgttgcg
     1321 ttttccctgc ccgctcgcca gcaccaccga cgccactgta aatccctccg gcatcttgtg
     1381 cagaaacacc gcaaaaaaca ttaccgctcc cagccacgtc gaaaccagga atcccgcggc
     1441 aatcgccacc ccatcaaaaa aagtatgaat cgtcatccct acaagtacgc tcgtccgtgc
     1501 gtgatgatgg ctcacctctt cacagtgggt ttcttcccca aaatgaaaat gcggcgccag
     1561 cgtgtgttcg aacagatgaa cgaggaagta gccgatcaac acatacagca gcgcgttttc
     1621 tccagaaagc cgtaccgact ccgggatcac ctcgacgaac gccaccgcca gcatgtaccc
     1681 ggcgcccagc gccagaaaat attgcagaaa tttccgcggc caatccttgt gaacaacaaa
     1741 atatccccca atcaaattcc ccgccgcggc cacgagcccc agccccgccg ccagcgccgt
     1801 ccgaaatgcc agatcatgtc ccattagtca gtagcacagg ctttagcctg tgcaagcgtt
     1861 cgatccatct cggcggtaac cagtaatcgc aaacttatcg tcgcctcgac ttctctgcgc
     1921 cctcttcctt ctctgtgaac tctgtgttaa ttcccgatct ttttctttac ttcgttattt
     1981 cattacttct ctcttcatcc gctgtgccga aagagaatca catccccttc ctgcacgatg
     2041 tactcttttc cctccaaccg cacctgcgcc ttctcccgcg ccgccgccag actccccgcc
     2101 gtcagcaatt cattccaccg caccacttcc gcccgtataa atcctctctc aatgtcgcta
     2161 tgaatcgccc ccgccgcctt caccgccgtc gcgccctttc gaatcgtcca tgcgcgcact
     2221 tccggctctc ccgccgtgaa aaactggatc aaccccagca aatcatacgt cgcgcgaatc
     2281 aaccggttca gccccggctc cttcaatcca tacgaagcca gcaactccgc cgcttccttc
     2341 tcatccatct ccgaaagttc cgcctccagc cgcccgcaaa tcgctatcac cgccgcattc
     2401 ggccgcccct gcagcgcgct caatttgtgc cgctccaccg ccgtgtccaa cttatccgct
     2461 tcgtcgtccc ccaggttcag cacgtagagc acgggccgag ccgacaaaaa caaaaatcct
     2521 ccaatcgact ttctctcttc cggcgtcaac tccagctccc gcagcgcctt ctccgcctcc
     2581 acatgcgcct tgcatttctc cagtgcgccc ttctcaatct ccagaatcgg atcgcgcttc
     2641 ttctttaaat ccttgtccac ccgctccagc cgccgcgtaa tctgatcgtg gtccgagagc
     2701 atcagctcca gatccagatt cgtcgcgtcg cgcaacggat cgatgctccc ctccgaatgc
     2761 ggcaccgacg gatcgtcaaa cacgcggatc acatgcgcaa tcacgtccac ttcgcgcaac
     2821 tgcgccaacg catcgcgcat gcgctccttc tgcatcccgc ccaaatctac gtaattcacc
     2881 gtcgcatacg taatcttctt cggcgtatac agcttcgcca actccaccag acgcggctcc
     2941 ggcaccttgg ccacgcccac atgcgtcgcc gacgccccgg ccttcccctc gacgtgtgcc
     3001 ttcgtcagta tccgaaacag cgtcgtcttc ccaacctgtg gcaatccaat aatccctgtc
     3061 tgcactccag ccctttcaac tcagtatttt ccgcccgcac aaactcctca tcctaacaca
     3121 ccccctacac tgtccattcg acgcagcgca caggctatta ctttgagttt gaagacggtc
     3181 ggtgtatgtt gctggtaccg gaacgcagcc ttccgcagta ttgaggctga cggagacagt
     3241 gcaatggcaa tgaaagaatc cattcgtagc ttgcgcctct atttcatcct gagtgccctt
     3301 gcaagtcttt gggtttgcgt agttaccttc acgcctgtct tgcgggcctc cctctctgcc
     3361 gtagtgatcg tggcggcggt cttcggtata gccttcctcg cgatgagctt ggcttttttg
     3421 tacgtgggat ttttccttcg ccggcttctc aaggattcct cgcatcggat cgtgatactc
     3481 atctacgtgg gcatggcgtg ggtagttatc tctttcttgg caaatctttt tggcggcatt
     3541 cagactcagg agattgtcat cgatgtgtta agcatgcttc ttggactgta tttgcttaag
     3601 aacatccgac gactggcagt agaagcacag gacccagcgg ccgatgccct tccctctact
     3661 gattagaccg aagcaaggtc cgtttgtggc cctccctcca gagaattgct aatctacttc
     3721 cccgttgccg aaatggagct ccagtgaccg cgacatcgca agtcctcgct ttcgaccttg
     3781 cagaagccac caagcatctc gccgatcgcg acgaatgcct gaagcgtcta atcgaagaag
     3841 cggtcccttt tcaaatcgac atcgccgacg cccaatcccc ctacgaggcc ctcctcgaat
     3901 ccatcgccta tcaatccatc tccggcaaag ccgccgccac catttacggc cgcattaaag
     3961 cgctcagcgc caccggccgg gcccccactc cgcaagaaat gctcaagctc cgcaagcctg
     4021 ttctccgcaa agccggcctc tccggcgcaa aagtcctcgc catgaaagac ctcgcgcaaa
     4081 aaaccatcga cggcgtcgtc cccaacctcg aacaagccga gaaactctcc gacgatgaac
     4141 tcgtcgagcg cctcgtctcc gtccgcggca ttggcgcctg gaccgtcgaa atgtttttga
     4201 tcttccgcct cggccgtccc gacgtcctcc ccatccacga cctcggcgtc aagaaaggct
     4261 ggtccgtcac ctacggcaaa aagcacatgc ccaagccgaa acaactcctc gccttcggcg
     4321 aacgctggcg tccctaccgc accgtcgcca gttggtacat gtggcgcgcc ttcgagcgcg
     4381 ccggctacgc cgccaccaac aaaatccgcc ccgccaaagt ccgcaggcgt aaacccgcca
     4441 acgtcacccg ccgcaaacgc aagcccttgt agctgcgggg ctttagcctc gcgaggtttc
     4501 gcctgtcctt accgcaactc ctgccggaga tatcgttccg ctcgtagctt tgccaccctg
     4561 tccgacagac tcgatatttt cccttgacca tccatcgctt ctggattatt ctttctcgta
     4621 gagaatttta tgattggact gcggcagctt gctgccgctt ttttatttgt gttcgagcga
     4681 gctgtcctgt tgttatgtct tacagtaaag tgcacccatc taagtcgttc agaacctgca
     4741 catccaggga ttctagtgtc aacgttcgag gggctttagg atgcgagcct cgcgattcgt
     4801 tgggcctggt ttcgcaccct acttaatagt tagacggccc gaaccatgcg gatagacaca
     4861 gacttccatg ctgcgtaccg agggcttgaa atacggatga aggctctcgc ggaggccgac
     4921 ggcgacgtgt ttctccccgt agctgaacca gaaggccccg cccattacgt cctgatcggc
     4981 atggagccgt ccttaggctg gtgggctcat tccgcagacg aggcacggtc gaaaatcgaa
     5041 gccggttacc gaaactttct tttctctgtt gaggacttca tccttcactt ttgcattcgg
     5101 cgctatctct gcgggccgga agagcaatac cacatcaccg atctgtcgaa gggagcaatg
     5161 ctcgtcgatc gtgccggcct cgcgagggtc gagcggtacg acagatggta cgggttattg
     5221 caggaagaga tagaccttgt ggcaacgcca aatgcaggca ttgcggcagt cggcagggac
     5281 gtggccctcc accttgagcg acgacgcttc gcaagaccct tcactcgggt agtccactac
     5341 tccagtcaag ccggacgcgc acgaagcgca gggatcattg gtcacgaaga aagtttcaag
     5401 gggttcatcg gctcagtatc cctggatgac ctggtcacca cggcggggca cgttctcggg
     5461 gcagcacgcg tgcccgccga gattcgcgac gagacactat cacgattggc gaggagccag
     5521 cttaccaact cccgtcagaa gttgatcttc aattacaagc tagccttcga attgatgcga
     5581 gcatgaggtc acgtggtgcc agcagaaaag cgccgcattt tgtacaattc tggtgcaata
     5641 taaccccttt agatgcaaca cttagggacc ttcttggatg tgttgcatct aaaggactta
     5701 cagaaacgct aactctttta gatgcaacac ttacacaaaa caggggggat gcgtggtgtt
     5761 atggttaact agcggctgat tttcaacggc ctgcgctagc taactctctc accataatat
     5821 acatagctgc aaatcccgcc aagagcacac cactttccag cgaaaccgaa atccggtgca
     5881 atcggccgaa ctccgcgagc agtgggctat ccgccggcgt cgcctggatc gaccccatct
     5941 gaacccgcag caccgccatc ttcgggctga ccgcatgctg cgacaccaca gtgagcagga
     6001 tcatcagcac aacgcaaagc gccgccggcg tcgtcaaact cgcaatcgtc cgcgtcctca
     6061 acaaacgagc cagcagaatc gcaatcccgc acaccactcc gatcatgtgc atccaccaca
     6121 atccgtaccc caccatggcc cccgcctagt cccggctcgc cagtatccga aacgcccccg
     6181 gcgccaccac aaagctcagg aacacgtcgc tccccagcca caggctcaaa cctagaaact
     6241 cgatcacacg aaggaaggtt gacatgcttg gattttagct agaatagctt ttggcgtgga
     6301 aaaaaagatg gtgggcgaca aaggacttga acctttgacc tctcccgtgt gaggggagcg
     6361 ctctaaccaa ctgagctagt cgcccattgc cgatagccgc gacagccggt accgccatct
     6421 atttgtaaca ggaaggacgc tggttttcaa tacatgccaa ctgccacctc ccaagtcgtc
     6481 tcgcagttgc agcgctccat cgcgcgcgcc atctatggca aggaagacgc catccagctc
     6541 gcattgatca ccctgctcgc gcgcggtcac ctgctcatcg aggacgttcc cggcgtcggc
     6601 aagaccactc tcgcacaagc gctcgcaaaa tccttccact gcagctttca gcgcatccag
     6661 ttcacgtcgg acctgctccc cagcgatgtt ctcggtgtga gcgtctacaa tcccgagtcg
     6721 cgcgaatttg aatttcgttc cggcccgatt ttcgccaacg tggtcctcgc cgacgaaatc
     6781 aaccgcacca cgccgcgcac gcaatccgct cttctcgagg ccatgaacga agcgcaagtc
     6841 accgtcgacg gcaagactct cccgctgccc cagccgtttc tcgtcatcgc cacgcaaaat
     6901 cctgtcgagc atcacggcac ctatcccttg ccggaatcgc agctcgaccg ctttctgatg
     6961 cgcatcaaaa tgggctaccc ctcgcacgaa accgaaaaag aaattctgcg caaacgcgtt
     7021 ggcgataatc ctctcgattc gctcgatccg gtcgcggatg tcagcgatgt aatggccatg
     7081 cagaacgccg tcgcgcacgt caaagtcgac tccagcctgc atgactacgc cctcgagatc
     7141 gtcaaccgta cccgcaagag cgagcagctc gcgctcggcg tcagcccgcg cggcacactc
     7201 atgctccagc gcgccgcgca agcccgcgcc tatctcgatg gccgcgacta ttgcctcccc
     7261 gacgacttca aacagcttgc cgtttccgtt ttctcacacc gcgtggtcgc cagttcgcgc
     7321 cacgcctcgt tgcagaaaaa atcggaaacc accgattccg ttcttcgcga tatcgttgaa
     7381 tccgtaccgg ttcctttgta ggttggagca attcggtgga atttattttc actctcaaac
     7441 aaccgtcatt ccaagcgaag cgcggttcct gcgctccgaa tgattttgca gagcgaggac
     7501 cgcttcctct ccccgcggtt ttcgcgtgga aaatctgctt tcgcctcttt ctgctgccaa
     7561 aatgagtgct ggcaaatcaa ccatcgcgca actctgggcc aaggccgacc gccccgccgt
     7621 ccgcgcgttc cttctctcca tcaccgcgct cacagcggca ctccttctgg cgctctattc
     7681 cggcgcagcc gcggaaatcg gccagcccgc tctcgccacc acttcggcgc ttgccgccct
     7741 cgccatcgcc ggctgggtgg gtgtgattct cgttcccatc ctggcgaagc gcacgccgct
     7801 ccgctggatc ggttacaaaa tggagtaccg catcaccctc gagggctgga tctatctcgg
     7861 cgggatcatt cttgtcgcac tcgcggcgct gaacaccgga aacaatcttc tcttcctgat
     7921 tcttgccagc ctcatcgcca tcgttttgat gtccggcatt ctctcttcca tcacgctctc
     7981 gggcatcgga atgcggctcg tattgcctga acatattttc gccggctcgc cggttcgcgc
     8041 catggtcgaa ctcgaaaatg aaaagctgac gctgccttcg ttctcactgc gcgtcgaagc
     8101 cgccaagaca aaacaggccc cgcccgcggc gatgctcgaa acgccggtct actttccata
     8161 tctcccaaaa catgaccgcg tgcagcaaac cattcccgtg acgtttccgc ggcgcggcgt
     8221 ctatcgtcag gaagctttcc gcgtcgtcac ccgtttcccg tttggctttc tgcgcaaggc
     8281 ccgccagatg aatttgaaaa ccgaagcact ggtctaccct tccgtggaac cgaccgcgga
     8341 atacctggaa attcttcccg gcttagaggg agcactcgaa agtctgacca agggccgcgg
     8401 ccaggatctc tacgcgctgc gcgactatat gccgaccgac agcgcgcgcc acgttcactg
     8461 gaaagcttcc gctcgctccg gttcactgat ggtccgcgaa ttcacccgcg aagacgactg
     8521 ccgcatcctg ctcgttctcg atcctcatct atccgaggag gaatcctcca ctctaaaacc
     8581 tggacagtgc gcgacgaacg agcgcttcga gcgcgccgtc accctctgcg ccagcttggc
     8641 ttggcatttt tacgaaaaca attctttgct gaaatttcgc agcgccggcg tggaaactcc
     8701 c
//
LOCUS       DASYVO010000141        20386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_58600, whole genome shotgun sequence.
ACCESSION   DASYVO010000141 DASYVO010000000
VERSION     DASYVO010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 20386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20386
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58600"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="VGR03_04680"
     CDS             complement(<1..1054)
                     /locus_tag="VGR03_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEV1993605.1"
                     /translation="MSDAKSKSATPAERKERFTTLSGIPLERVYCPDAFVDSEMEGAI
                     GFPGEYPFTRGIYPTMYRGRFWTMRQYAGFGSAVESNQRYRYLLSKGQAGLSVAFDLP
                     TQIGMDSDHPLALGEVGKVGVAIDSLEDMETLFDGIPLEKVSTSMTINATAAILLCLY
                     VAVAKRQGASLQKLSGTVQNDILKEYIARGTYIYPVRPAMRIVTDIFAWCRGNLPKWN
                     TISISGYHIREAGSTAVQEVAFTLADGIAYVQAALDAGLRVDEFAPQLSFFFNAHNDL
                     LEEIAKYRAARRLWAKIMAERFGAKDPRSLMLRFHAQTAGSSLTAQQPENNIVRVAIQ
                     ALAAVLGGCQSLHTNSM"
     gene            complement(1121..1375)
                     /locus_tag="VGR03_04685"
     CDS             complement(1121..1375)
                     /locus_tag="VGR03_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993606.1"
                     /translation="MSTAARAATAPQQQGGFRRLWRVMRQLFHEVTGAVFAVLALGWL
                     NAALRAWTRDVARWLIGIAVAVALLFLFFAVTSFRKARKL"
     gene            1557..1913
                     /locus_tag="VGR03_04690"
     CDS             1557..1913
                     /locus_tag="VGR03_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993607.1"
                     /translation="MQLDDHLKNLLRELGHAINDTVSDSDRITGAIAGVRAHGYDIVL
                     KLDATIGLAKRDAAAAEAAKLTTQDRRFLESLRIQVDQESFLEENDKKPAPRLAMTPQ
                     DVRFLKSLRITTDDAE"
     gene            1910..2317
                     /locus_tag="VGR03_04695"
     CDS             1910..2317
                     /locus_tag="VGR03_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993608.1"
                     /translation="MKRIVKVVGFGLPLMLAAAGLFSSPAAAQVPAAAVPIIVDTAVP
                     IIVGAVKPKPKHTGLDKFEGFVMHANIVQITVRAKGSDMAIRTFPLSQEASAKMQKIV
                     DMGGYQYGDKVTVFYEPDTTKAHKVKGKPSKPI"
     gene            2334..3143
                     /gene="amrB"
                     /locus_tag="VGR03_04700"
     CDS             2334..3143
                     /gene="amrB"
                     /locus_tag="VGR03_04700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEV1993609.1"
                     /translation="MLRQPAVAGRFYPSDPAELTALIRKYTSADPGRSPISVRACLVP
                     HAGYVYSGHVAGAVLARIALPRKIIILGVRHHPRGASAAILSNGAWRTPLGDARIDGA
                     LAEELKKACSLLREDSVAHSTEHSLEVQLPFLQVLAPDFTFVPVALGTVRFESLVTVG
                     EAIARVLETSKENVLLLTTSDLNHYEDDATTRIKDRKAIEQLLALEPRGLYDACRNED
                     ISMCGLGPAAAMLTALNALGVKKSELVNYATSADVSGDRNAVVGYAGMIFS"
     gene            complement(3227..4951)
                     /locus_tag="VGR03_04705"
     CDS             complement(3227..4951)
                     /locus_tag="VGR03_04705"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEV1993610.1"
                     /translation="MTSRKLIPSLLVVLLSSLVVGAQPAAADSSHARIIRLSFVQGDV
                     RFARETHGDPLADSNTTWETAGLNLPIRQGYVLATDKGRAEVEFENGAVAFLKENTVL
                     EFYDLSLNDSARTTRLVLRQGSASFYVNPAGGDYFSVTGGDFTVEADGRSEFRIDNYD
                     DGSTVEAHKGRVSVLHNKNTTRLEKGQSLSVKASDNSSPSVGRVPEEDDFDRWVSGRI
                     DSVSTATTAALKYTGSPNYVPGFADLYTYGAFSNCGAYGYGWRPFGVGFGWSPFTNGQ
                     WIMDPAFGWTWVSSQPWGWAPYHYGGWLFDASCGGWFYSPPVLYGNPGYPVGPRKRFP
                     RGVHPPHPIYRPVTAVFVRQGGKNGIVPMHPLDKKGKSPLNLERGVFSGVGTKGVSAQ
                     VISVERGQKWETLKSAPREGLSGSLVATGPPTRVSRTVVEGKSGIRAVTPGKDSSIAY
                     DPHERRFVNTNSTAASSSVTGKEIRVENGRTAAGTSTPTAVTPPRSPVTPPATRSASS
                     PPARNSVPPPAPRASGGERGSSSGRGGSTWSGSASSSSSGTGRSSSSSTSSTSSASSS
                     HPSSGRPH"
     gene            5245..5321
                     /locus_tag="VGR03_04710"
     tRNA            5245..5321
                     /locus_tag="VGR03_04710"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5279..5281,aa:Val,seq:gac)
     gene            complement(5650..7071)
                     /locus_tag="VGR03_04715"
     CDS             complement(5650..7071)
                     /locus_tag="VGR03_04715"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004938.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV1993611.1"
                     /translation="MKARLQVMKFGGTSVGDAACIARTAQIIAKAAKESGCIAVVSAM
                     SGVTNRLIEAATKAQAGNAGEAAAIVEALRQQHEAALTSLIPREEERARIRQRIEEVL
                     AEGRRLCEGTALLCELTPRTLDAISSLGERLSAPLVAAAVRELGLPSEAIEATELIVT
                     DAFHGGAEPQMELTRGKSQARLRPLLEKGIAPVVTGFIGATAEGQLTTLGRGGSDYSA
                     TILGAAMDADEVIIWTDVDGVLTADPRLVAEARTIPVISYREAAELAYFGAKVLHPKT
                     LNPVVQAAIPVWIRNSFAPERLGTKITPEGRSIGGGVKALTAIRDVTLISVGGPGIVG
                     LPDVLGRTFSTTAEVRANVLLISQSSSQNDICFIVSTVDAQRTVEALRKEFAQDLAHQ
                     KVEHITVDSNVAIVAVVGENMRGTPGIAGRTFNALGRENVNLIAIAQGSSECNISFVI
                     EEQAVKTALNTTHREFGLEASPN"
     gene            7363..8658
                     /locus_tag="VGR03_04720"
     CDS             7363..8658
                     /locus_tag="VGR03_04720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV1993612.1"
                     /translation="MPAAAAEAPANPDSPLETTGYRNLYILLLVCGFALRFGFVLWKR
                     TYLRIPGSFQPFGPEVCSIAEHIVLGKGFSSPFYQDTGPTAWIAPVYPYLAALVFRLF
                     GIYSVASAIVLLGIQCIIAAATGITIHVLGRRTLGAQVGFFAAWIWALSPIFFRWTVS
                     WIWDFTASAFLLSAVFIVTLDVAEKNTRKHWLSLGALWAVIALTNPALLSVMPFTFLY
                     AGFVNHRSFRPWFASAALAGVLFAALVSPWLIRNELVFGRPVFFRSNFWFEFHLGNYH
                     FSNGMGFSGKHPTANAAEFKKYAAMGEMGFLDYYKKDSLRFVREYPSEFLDLTLHRTW
                     WFWDGTSLLYQSREWWKPWEFWPLSLGGWLGLLFVLTRRPRGWLLFAAALLVYPIPYY
                     FVYPVSKYRHAIEPELLLLSVYFIFVLWSEIRALAHHKA"
     gene            8793..10886
                     /locus_tag="VGR03_04725"
     CDS             8793..10886
                     /locus_tag="VGR03_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV1993613.1"
                     /translation="MKTRRVLGLTLVVFAIGVAIALRSSADSTAKPGDVNEARVLADA
                     ATGNNWLVNGRTNDSKHFSPLKQINDQNVGNLGLAWYLDYDGLMGVVSEPIVVDGVIY
                     VSAPLSKVYAVEASTGKLLWKYDPKIRLDQALNGSYSARTNGGVAVWNGKVYVGTGDC
                     RLIAIDAATAKKIWEATVCEPLQTGITGAPRVAKGKILMGYNGSDDGVRGALAAYDAE
                     TGKEVWRFWTVPGDPSKGFETKELEMASKTWFGEGSWKIGGGDVWHAITYDDATGLVI
                     FGTAGADVGYGEMTGVKITGEKLFSQCIIAVKADTGEYVWHYQTGTENVHSENNQIMM
                     ADLMINGEKRHVVLTAPKNGFFYILDAKTGKLLSAKRLVKTTWATSYDLQTGRPVLIP
                     ASQGGGRQWTVHNWWPMSYSVLTGLVYIPSTDRRSDTKAAVEAGEGGEGLFGRLIAWD
                     PIAETARWSVEEQISVNGGVFSTAGNLVFQGQGTGEFAAYAADTGKKLWSIQTKSAID
                     SIPVTFSVNGEQYVITSVGWGSGSRLFAPARTMATPESKRGPNRLYAFKLGGKVPFPA
                     PHIEVPPVPKPPEQKVSEEIIRKGEKLYVSFYCEGCHSPFIDGSGAWVEDGAIPDLRY
                     APSEVHKDWYGIVLGGSHWSKGMPGLADPPKFAFPKAHMTVAEADAVHAYVISQAWKA
                     YKGEQEIAHSKRENY"
     gene            complement(10883..11245)
                     /locus_tag="VGR03_04730"
     CDS             complement(10883..11245)
                     /locus_tag="VGR03_04730"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEV1993614.1"
                     /translation="MGSTLAKIYVVLSGAILLVVGVVGFFRHEMFKLTFPPAHNVFHL
                     VSGVIALWAGLGKRADGPRLFGLIFGAIYTLVAIAGFAGLRDLGPIQLDLNTHFNFIH
                     LVVGLLSLAAGFASAKAS"
     gene            11644..11769
                     /locus_tag="VGR03_04735"
     CDS             11644..11769
                     /locus_tag="VGR03_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993615.1"
                     /translation="MKTTEPNPNTRGWWTSILSDIHFWVPVAVLIGGLLLLRFIR"
     gene            11788..13092
                     /locus_tag="VGR03_04740"
     CDS             11788..13092
                     /locus_tag="VGR03_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEV1993616.1"
                     /translation="MPPTSPSQFPPAIKDASRSKKLQLLGILCGFAAGAWLGAAEAPT
                     KLVSIGISPMVISLIMVIGVFLARWSLPALILGTSSVRADVRRAPHLIIWALLAGCLW
                     AVANTLTIFAIRDIGLSIAFPLWNSNSLLGILWGFLFFNELRQAGWRRLAGVLGGALV
                     MCAGAAFLAVASSSRGPAAHSLHGVWAALGAGILWGTMYIPYRKAYLTGMNPLSFVTF
                     FSFGELGMMTVLAVSSLGLASLWQELLSARSVIFWLMLGGFVWVIGDVFQQYAAKYVG
                     ISRGIPLSNSNQLWGLLWGILVFGELHGRSSSIYLEVIGGSLLMMLGVCAIAFSSATG
                     QEQTCWKEAAIRESDRYGVAADFVEARMDGRQLLGESKPSRSALDWFLVVAATSVFVI
                     FEAMARVPRMSLHWVPAALLTALLFLFLAVCGLALWRTTRFN"
     gene            13150..14097
                     /locus_tag="VGR03_04745"
     CDS             13150..14097
                     /locus_tag="VGR03_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEV1993617.1"
                     /translation="MTQSAKLDVVGVGLNATDTLIPVAHYPERGSKVEFRSAKVLPGG
                     QVATAMIACQQWGLRTRYVGKVGDDSAASLHRAEFARLGVEAHLFTAPGCPSQQAFIL
                     VDDSGERTVLWKRDDRLTLHPEELQRDWIVNARALHVDGHDTAAATAAAGWARAAGIP
                     VVADVDDLYPGVETLLKSVDYLITSRDIPGRLMEEPDLKKALPALLKRFGSRLTAATL
                     GEEGVLAWDGTQFHYAAAFHVQPVDTTGAGDIFHAGFIYGLLQGWPLHRQLDFACAAA
                     ALNCTAIGARGGIQSIEVIEHLIATGVRHAQAYEISGLT"
     gene            complement(14389..14799)
                     /locus_tag="VGR03_04750"
     CDS             complement(14389..14799)
                     /locus_tag="VGR03_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993618.1"
                     /translation="MTNDRYLIVSYFAVAALAIAFAGATWLYLRRSFGGITQTLSTGN
                     LSRILKAMFPAGLFLPAFLGFVSVIYRGCGKSYGEIVKERSYLIQKNQEQISSILLWI
                     AAALLFWNLVILFALKFARTSSQASGESPDIRLP"
     gene            complement(14789..15145)
                     /locus_tag="VGR03_04755"
     CDS             complement(14789..15145)
                     /locus_tag="VGR03_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993619.1"
                     /translation="MGANTYAFIALFVLSAALAIGLFLPLRDALRGLLQRTVHLPDGV
                     TFYLRSFFVLLFFSALGGALGFTFDLKPGSHFMEYVWKVASGLSNVLEQMLLYLGGYL
                     VLTTVILATLKIKDDK"
     gene            15450..17606
                     /locus_tag="VGR03_04760"
     CDS             15450..17606
                     /locus_tag="VGR03_04760"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEV1993620.1"
                     /translation="MADPVISPPEVAFTSPLEHSRRQLEALLEVSEAIAQQRDLPALF
                     HDLAGRLHSVVDFDFLTLVLHDASRNVMRLHVLETRIPGEKHTGTETPVEGHPSGWVW
                     QTQQAFVVSDTEEETRFPDFMQRLREYNVRSLAIIPLTTAQRRLGAMGFGRIVPQGIT
                     DTELQFMQRVASQVAVAVDNALNLETSQAYQQQLARERDRLRVLLEVNNVLITSRELP
                     ELFRGIVNTLERVIHHDYTSLALLDSQTGLLKIHALDFPGNQSLFKQEITISRDVSPS
                     GHAISTGQPLLARGAELDRYTSEIIRVLRSEGIQTVCCVPLVTHGHTFGSLNLASRRL
                     DAFTLQDVELLQQVCAQVAIAVENALAFKEIDVLKNKLAEEKLYLEEEIRSEFNFEEI
                     IGESPALKRALAQVELAAPAGTTVLLLGETGTGKELFARAIHNLSPRRDRTFVKINCA
                     AIPSGLLESELFGHERGAFTGAINQKIGRFELADRGTLFLDEVGDLPLELQPKLLRVL
                     QEQEFERLGGNRTQRVDVRVVAATNQDLSKRVAERAFRSDLYYRLNVFPIQIPALRER
                     AEDLPLLVRYFVQRFSRQLNKGVEYIPADAMDALIHYSWPGNVRELENLIERAVLLSP
                     GKELRIPFAELKSAALATAAGAEPSSSFTSFASLTSFTPSSISTLEEAERQHILRALK
                     QTQWRIAGSKGAAVLLGMKRTTLQARMRKLGIRRSV"
     gene            complement(17654..18604)
                     /locus_tag="VGR03_04765"
     CDS             complement(17654..18604)
                     /locus_tag="VGR03_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEV1993621.1"
                     /translation="MTKRILLVIATVVLAGGLGAPAYGWNTYGHMAVAYVAYQKLTPQ
                     AKARAKDLLSQNPKFNEWMSWVPASASPADKDMMVFMLAAQWADEIKGDSAYSDDGSN
                     GGNTPDGVSSSQNTGYRDLLKHKYFHFVDTPFTNDGSTLPAIPTPNAQERIALFRGVL
                     ASNSDDKLKSYDLTWLLHLVGDVHQPLHSATRVSSADPGGDNGGNKVKLNCSGCAKEL
                     HAFWDDLFGKGTSAQAVIKAAKKLPAADAGQAAKSDEKDWIAESFQAAQQTVYQPPVA
                     AGDGPFSLTSGYKSKAKTLAKQRIALAGARLANLINNELK"
     gene            19005..>20386
                     /locus_tag="VGR03_04770"
     CDS             19005..>20386
                     /locus_tag="VGR03_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993622.1"
                     /translation="MKIASVFLAFTGSFGMLAAPALGQAPPPANPAKSVSRTTKAVNY
                     RRAGGSTKIDFQGSELMHQASGEAKVQNKGNRTEIDAKFVGLDEATKFGLEYLTYVLW
                     AVSPGGRAVNLGEVVLKNGSGEVKAISDMQTFGMIVTAEPYFAVTQPGNTVVLENVFG
                     PATLGKVENIDASYELLGRGIYSSSNTKIENAIFGIDRKTPLELFEARNSVRIAHIAL
                     ADKYAASTLAKAEQQLKSAEEVYARKSDKKSVIAAAREVVQTAEEARVMAVKLKAEED
                     AQAKAAAEKKSAEQREAKARADAEAEARRRQEAEQARVQAEAAQAEAVRMKQQAEKAA
                     QEAARQQQEAERQKQEAEQARAAAQAQQQAAVEQQHAAEAETEKARQAAAQAEAEKVQ
                     LRAQLLNQLNSILQTRDSARGLIVNMSDVLFDTGSSTLKPGAREKLAKISGILLAHPG
                     LTLQIEGHTD"
BASE COUNT         4053 a         6284 c         5755 g         4294 t
ORIGIN      
        1 ccatggaatt ggtgtgcaag gattggcagc caccgaggac ggcagcgagc gcttgaatcg
       61 ccacgcggac gatgttgttt tctggctgct gcgcggtaag ggaggagccg gcggtttgcg
      121 cgtggaagcg cagcatcagc gagcgcggat cttttgcgcc gaagcgttcc gccataattt
      181 ttgcccacag gcggcgggcg gcgcgatact tggcgatttc ttccagaaga tcgttgtggg
      241 cgttgaaaaa gaaagaaagc tgcggcgcga attcatcgac gcgaagtccg gcatcgagcg
      301 cggcctggac gtaggcgatg ccatcggcga gcgtgaaggc gacttcctgg acggcggtcg
      361 aaccggcttc gcggatgtga tagccggaga tggagatggt gttccacttg ggaagattgc
      421 cgcggcacca cgcgaaaatg tcggtgacga tgcgcatcgc ggggcgcacg ggatagatgt
      481 acgtgccgcg ggcgatgtat tccttcaaga tgtcgttttg cacggtgccg gaaagttttt
      541 gcaggctggc gccctgtcgt tttgcaacgg ctacatagag gcagagtagg atcgcggcgg
      601 tggcgttgat ggtcattgag gtggagactt tttcgagtgg aatgccgtcg aagagcgtct
      661 ccatatcttc gagggaatcg atggcgacgc caacttttcc gacttcgcct aaggcgagcg
      721 gatggtcgga atccatgcca atctgtgtgg gaagatcgaa agcgacagag agaccggcct
      781 ggcccttgct cagaagatag cgatagcgct ggttggattc gacggcgcta ccgaagccgg
      841 cgtactggcg catggtccag aagcgtccgc ggtacatcgt tggatagatg ccgcgggtaa
      901 aaggatattc cccggggaac ccgatcgctc cttccatctc ggagtcaaca aaggcgtcgg
      961 ggcagtaaac gcgctcgagc ggaatgccgg agagggtggt gaatctctcc ttgcgttcgg
     1021 cgggtgtggc ggatttggat ttcgcgtcgg acatgattgt gtgctcgatg ggttgaagcg
     1081 gcgattcggc aaacccaaaa ccgccggctg gaagccggcg ctacaacttc ctggctttgc
     1141 ggaacgacgt tacggcgaaa aacagaaaca gcagcgccac ggcgacggcg atgccgatga
     1201 gccagcgagc aacgtcgcgc gtccaggcgc gtagcgcggc gttgagccaa cccagcgcga
     1261 ggacggcaaa taccgcgccg gtgacctcgt gaaataactg cctcatcacg cgccagagcc
     1321 gccggaaacc gccttgctgc tgcggcgcgg tagctgctct tgcggccgtt gacataagag
     1381 catcttaacg caaaacgcgg cagacatcag gaatcggggc cgggtttcgc tcaacatggg
     1441 ggccatagaa cctgaacgaa ggtactgtgg acggattaga gacaacggag cgaagctctc
     1501 ttgtatccaa ctatacgtgg gcctagaatg gccaaagaag taagggagaa gccgccttgc
     1561 aactcgacga tcacctgaaa aacttgctcc gcgaactagg acacgcgatc aatgacacgg
     1621 tgtccgattc cgaccgcatt acgggtgcga ttgccggagt tcgcgcgcac gggtacgaca
     1681 tcgtgctgaa gctggacgcg accatcgggc tggcgaagcg cgacgcggcc gcggccgagg
     1741 cagcaaaact gacgacgcag gatcgccgat ttctggagtc gctgcggatt caggtggatc
     1801 aggaatcttt tttggaagag aatgacaaga agcctgcccc gcggctggcc atgacgcccc
     1861 aggacgtgcg atttctaaag tcactgcgga ttaccacgga cgacgctgaa tgaagagaat
     1921 cgtcaaagtt gtcgggttcg gactgcctct gatgcttgcg gcggcggggc tcttttcttc
     1981 gcccgctgct gcgcaggttc cggcggcggc ggtgccgatt atcgttgata ccgcggtgcc
     2041 gatcatcgtc ggtgccgtga agccaaagcc aaaacatacc ggacttgata agtttgaagg
     2101 cttcgtgatg cacgcgaaca tcgtgcagat caccgtgcgc gcgaaaggga gcgacatggc
     2161 catcaggacg ttcccccttt cgcaagaggc gtccgcgaaa atgcagaaaa tcgtcgacat
     2221 gggcggctac cagtacggcg acaaagtgac cgttttttac gaacccgata cgacgaaagc
     2281 gcacaaggtc aaagggaaac cctcgaagcc catttgattc gcggagcggc aagatgcttc
     2341 gccagcctgc ggttgccgga cgcttttatc cttctgatcc tgcggaacta acagcgctca
     2401 ttcgcaaata caccagcgcc gatccgggcc gatcgcccat ttcggtaagg gcgtgccttg
     2461 tcccgcacgc cggttacgtc tattcgggac acgtggctgg cgcggtgctc gcgcggattg
     2521 cgctgcccag gaaaattatc attctcggcg tgcggcatca tccgcgaggt gcgtcggcgg
     2581 cgatactctc gaacggcgcc tggcgcacac cgctgggaga cgcgcggatt gacggggcgc
     2641 tggcggaaga gctgaaaaaa gcctgctcgc tgctgcgcga ggatagcgtg gcgcacagca
     2701 cggagcattc gctggaagtg cagttgccgt ttcttcaggt gctggcgccg gacttcacat
     2761 ttgttcccgt ggctctggga accgtgcgat tcgaatccct ggtgactgtg ggtgaagcga
     2821 tcgcacgggt gctcgagact tccaaagaga acgtgctgct attgacgacc tcggacttga
     2881 atcattacga agatgacgcc acgacacgaa tcaaggaccg caaagccatt gaacagttgc
     2941 tggcgttgga accacgcgga ctctacgacg cctgccgcaa cgaagacatt tccatgtgcg
     3001 ggctgggacc ggcggcggcc atgctcacgg cgctgaacgc gctgggtgtg aaaaaatcgg
     3061 aattggtgaa ctatgcgacg tccgccgacg tgtccggcga tcgcaacgcg gtcgtgggct
     3121 acgcgggcat gattttttcc tgactcacgt gaataacaat ttgccgcggt gcaataaaaa
     3181 acggctcccc attggggagc cgtttctacg atcgttccac agtagattag tgcggccggc
     3241 cggaggacgg gtgcgaggaa gaggccgaag aagtggagga cgttgatgac gacgaagatc
     3301 gtcccgttcc gctgctgctc gacgaagcag agccgctcca agtgctgcct cccctgccgc
     3361 tggaacttcc acgttcgccg cccgaggcgc gcggtgctgg cggtgggacg ctattgcgtg
     3421 caggcggaga ggaagcgctg cgcgtcgccg gaggagtcac aggagagcgc ggcggtgtga
     3481 cggcggttgg tgtcgaagtg ccagccgcgg tccgtccatt ttccacgcgg atttccttcc
     3541 cggtcacgga agacgaagcc gcggtagaat tcgtattcac aaagcggcgt tcgtgcggat
     3601 cgtacgcaat tgaagaatcc ttaccgggcg tgactgcacg aattccggat ttgccttcaa
     3661 ccaccgtccg cgaaacccga gtcggcggcc cagtggccac caggctgccg ctcaggcctt
     3721 cgcgcggagc cgacttcaat gtttcccatt tttgtccgcg ctcgacagaa atgacctgcg
     3781 cgctcactcc cttcgttccc acgccagaga aaacgccgcg ctccaaattc aacggactct
     3841 ttcctttttt atcaagcgga tgcatgggca cgatgccatt cttgccgccc tggcgaacga
     3901 acaccgccgt caccggacga tagatggggt gcggcggatg aacgccccgg gggaaccgct
     3961 tgcgcggacc gaccgggtac cctgggtttc cgtagagcac cggcggggaa taaaaccagc
     4021 cgccgcagga ggcatcgaag agccatcctc cgtagtgata gggggcccag ccccacggct
     4081 gagagctgac ccaggtccag ccgaacgcag gatccatgat ccattgccca ttcgtgaatg
     4141 gtgaccagcc aaaccctaca ccgaacggtc tccaaccgta tccataagct ccgcagttgg
     4201 aaaaggcgcc ataggtgtaa aggtccgcaa accccggaac gtaattcggt gaacctgtgt
     4261 attttagcgc cgcggtagtc gccgtggaca cgctgtcgat gcggccggaa acccagcgat
     4321 cgaagtcatc ttcctctgga acgcggccga cgctcgggga agagttgtcg cttgctttca
     4381 ccgagaggga ctggcccttt tcgagccgcg tcgtgttctt attatgcaaa acggacacgc
     4441 ggcccttatg ggcctcgaca gtgctcccgt cgtcataatt atcaatccgg aattcgctcc
     4501 ggccgtcggc ttccacggtg aagtcgccgc cagtgacgct gaagtagtcg ccgcccgcgg
     4561 gattcacata gaaggaagcg ctcccctgcc gaagaacgag gcgcgttgtc cgcgccgaat
     4621 cgttgaggga aagatcgtag aactccagga ccgtgttctc tttgaggaaa gccactgccc
     4681 cgttctcgaa ttccacttcc gcgcggcctt tgtccgtggc aagaacgtaa ccttggcgga
     4741 tcggcagatt cagcccagcg gtctcccaag ttgtgttgga gtccgcaaga gggtcgccgt
     4801 gcgtctcccg ggcgaatcgg acgtcgccct gaacgaagct cagtcggatg atgcgggcat
     4861 ggctggaatc cgctgcggcg ggttgcgcgc ccaccaccag cgaactcaac agcacgacca
     4921 aaagggaagg aatcagtttg cgggaagtca tattcacccc ctcacgggga agtggtccaa
     4981 atcgttagac ggtgagaagt gcgggcaggt tgcgtgccga attgcgtacc aggttacgtg
     5041 caatgtgccc taaaccatgg tctcgccggg ggatggcggc gtcaaggggg gactgcgatt
     5101 cgtgggtaac gaactgtaga agctgtattt tgaaacggga ctaatgtaca aatgagataa
     5161 attttgtgtt ctgtccggat tgcgctacgc cagcccccag ccactgcgtt tgctatgcca
     5221 cccgtgctaa actttatgga ttctaggccc gtagctcagt tggttagagc gctaccttga
     5281 cacggtagag gtctggggtt cgagtcccca cgggcctacc attcgttgct attccgcaat
     5341 ggccttcgtt tacatcttgc agagtgaaac aaccggacgc ttctatatcg gctcgacgga
     5401 tgatctggaa agacgcctca ttgaacatct acgcgggcat agcccagcta cccgaggtat
     5461 ggggccgtgg aagcttgtct acaaggaaca attcgatgct cttattgagg ctcggcgacg
     5521 ggagttggag atcaagcgat ggaaatcggc caaactaatt caagcactta ttaaaagctc
     5581 agctggttag agcgggccga ccgggtcggc caggtctggg gttcgagtcc ccacaagcct
     5641 gccattccat cagtttggcg atgcttccag gccgaactcg cggtgcgtcg tattcagagc
     5701 tgttttcacg gcttgctctt cgatcacgaa ggaaatgttg cattccgaag atccctgggc
     5761 aatggcgatc agattcacgt tctcacgccc cagcgcattg aaggttctgc cggcgatgcc
     5821 cggggtccct cgcatgttct cgccgaccac ggccacgatc gcgacgttcg aatcgacggt
     5881 gatgtgctcc actttctggt gcgccaggtc ttgcgcaaac tctttgcgca gcgcttccac
     5941 ggtgcgctga gcatccacgg tggaaacgat gaaacagata tcgttttgcg aggacgattg
     6001 cgaaatcagc agcacgttgg cgcgcacttc ggcggtcgtc gaaaacgtcc gccccaacac
     6061 atccggcaag cccacaattc ccggcccgcc cacgctgatg agtgttacgt cgcgaattgc
     6121 cgtcagcgct tttacgccgc ctccgatgct gcgcccttcc ggcgtgatct tggtgcccaa
     6181 tcgctcgggc gcaaaactgt ttcgaatcca cacgggaatc gccgcctgca ccacggggtt
     6241 cagcgttttc ggatgcagca ctttcgcgcc aaagtaagcc agctccgctg cttcgcgata
     6301 agagatcacc ggaatcgtgc gcgcctccgc caccagccgc gggtctgcgg tcaacacgcc
     6361 gtcgacgtcg gtccagatga tgacttcatc cgcatccatc gccgcgccca ggatggtcgc
     6421 ggaatagtcg gagccgcctc gccccagcgt tgtcagttgc ccttccgccg ttgcgccgat
     6481 gaagcccgtc acgaccggcg cgattccttt ttccaacaac ggccgcaacc gggcctgcga
     6541 tttccctcgc gtcaattcca tctgcggttc cgcgccgccg tgaaatgcgt ccgtcacgat
     6601 cagctccgtt gcttcgatcg cttcgcttgg cagccccagc tctctgaccg cggccgccac
     6661 cagcggcgcg gagagccttt cccccaggct cgaaatcgcg tccagagtgc gcggcgtgag
     6721 ttcgcacagc aacgcggttc cttcgcagag ccgccgccct tccgcgagca cttcttctat
     6781 ccgctgccgg atccgcgcgc gttcttcctc gcgcgggatc aggctcgtca gcgcggcttc
     6841 gtgttgctgc cgcagcgcct cgacgatggc tgcagcctca ccagcgttcc ctgcctgcgc
     6901 cttcgttgcg gcttcgatca gccggtttgt caccccgctc atcgccgaaa ccacggcgat
     6961 gcagccgctc tcctttgccg ccttggcgat aatctgcgcg gtcctggcga tgcaagccgc
     7021 atcgccgacc gaggtaccgc cgaatttcat cacctgtagc cgcgctttca tcttttcctg
     7081 cccgcctccc tcaatggacc ggcaaaccat ttttcttggg cgaaacttgt tcttcttact
     7141 tccctggttt tttctgcact gagtttacct tgtattccag ctgcgcttcg ctttcccttt
     7201 caactgtacg accttgggca ggtcttttgc attattcagc ttctcatccc agctcttccg
     7261 tcttctttgc gaaccgctat cctgccctcc cagtgccgca taagaatgta gattccaact
     7321 ccaccttgcc gcctcgcgag acgatctttc acaataagcg acatgcctgc cgccgccgcg
     7381 gaagcccctg caaatcccga ctcgccgctg gagacgactg ggtatcgcaa cctctatatc
     7441 ttgctgctcg tctgcggatt tgccctgcgt ttcggattcg tcctctggaa aagaacttac
     7501 cttcgcatcc cgggttcgtt tcaacctttc ggcccggaag tttgcagcat tgccgagcat
     7561 atcgttctcg ggaagggatt cagttcgccc ttttaccagg acaccggccc aaccgcctgg
     7621 atcgcccccg tttatcctta tttagccgcc ctagtcttcc ggctcttcgg catctactcc
     7681 gttgcttcgg cgatcgtgct gcttggcatt cagtgcatca tcgccgccgc caccggcatt
     7741 actatccacg tcctgggccg ccgcacgctg ggtgcgcaag tcggattctt cgcggcgtgg
     7801 atctgggcgc tcagccccat ttttttccgt tggacggttt cctggatttg ggattttacc
     7861 gccagcgctt ttcttctttc cgcggtcttc atcgtcacgc ttgatgtcgc ggaaaagaac
     7921 acgcgcaagc actggctatc gcttggcgcg ctctgggcgg tgatcgcgct caccaatccc
     7981 gctctgctca gcgtcatgcc gtttactttt ctttacgcag ggtttgtaaa ccaccgctcc
     8041 ttcaggcctt ggttcgcttc cgcggcgctt gccggtgtgc ttttcgcggc gctggtttct
     8101 ccctggctga tccgcaacga actcgtcttc ggccggccgg ttttttttcg cagcaacttc
     8161 tggtttgaat ttcaccttgg caactaccac ttcagcaacg gcatgggttt cagcggcaag
     8221 catcccaccg ccaatgccgc ggagttcaaa aaatatgcgg ccatgggaga gatgggcttc
     8281 ctcgactatt acaagaaaga cagcctgcgt tttgtccgcg agtatccttc cgaattcctc
     8341 gatctgacgc tccatcgcac ctggtggttc tgggatggca cttcgctgct ctatcagagc
     8401 agggaatggt ggaagccctg ggaattctgg ccgctgtctc tcggcggttg gcttggactt
     8461 ctttttgttc tgacgcgacg cccgcgcggc tggctgctct tcgccgccgc tctactcgtt
     8521 tatcccattc cttattattt cgtctatccc gtctcaaaat accgccacgc catcgagccc
     8581 gaacttcttc tgctctccgt ctatttcata ttcgttctct ggagcgaaat tcgcgcgctc
     8641 gcacatcaca aagcgtaact tcgcattcgt tgacatttct tccgccgcgc acctaacatg
     8701 gcacgcagaa actcctcgcc aatcaggttc gggcaagcga tctcgcatcg gtgcggcgga
     8761 tgttcggcgg ctcattcgaa aggcggtctg ccatgaaaac acggcgcgtt ctcggtctga
     8821 ccctcgtcgt ctttgcgatc ggagttgcca ttgcgctgcg ctcctcggcg gacagcacgg
     8881 cgaagcccgg cgacgtgaac gaggcgcgcg tgttggccga tgccgccact gggaacaact
     8941 ggctggtaaa cggacgcacc aacgattcga aacacttcag cccgctcaag cagatcaacg
     9001 atcagaatgt cggcaatctg ggccttgcct ggtatctgga ttacgacggc ttaatgggcg
     9061 ttgtttccga gccgatcgtt gtcgatggcg tcatctacgt cagcgctccg ctctccaagg
     9121 tttacgccgt ggaagcttcc acgggaaaac tcctctggaa atacgatccc aaaatccggc
     9181 tcgatcaagc gctgaacggc tcttattccg cgcgcaccaa tggaggagtg gctgtatgga
     9241 atggaaaagt ttatgtcggc acaggcgact gccgcctgat tgccatcgat gctgcgacgg
     9301 cgaagaagat ctgggaagcc acagtctgcg agcccttgca gaccggcatc accggcgcgc
     9361 cccgagttgc caaggggaaa atcctcatgg gctacaacgg ctcggatgat ggcgtgcgcg
     9421 gcgctctcgc cgcctatgac gcagagacgg gcaaggaagt ctggcggttc tggactgtcc
     9481 cgggcgatcc ctcgaaaggt ttcgaaacaa aagaactgga gatggcctcg aaaacctggt
     9541 tcggcgaagg ctcatggaag attggcggcg gcgacgtgtg gcacgccatc acttatgacg
     9601 acgcaacagg cctcgtgatt tttggcaccg cgggagcgga tgttggctac ggtgaaatga
     9661 ccggagtcaa aattacagga gaaaagctct tttcccagtg catcatcgca gtgaaggcgg
     9721 acaccggaga atacgtctgg cactaccaga ctggcaccga aaacgttcac tccgaaaata
     9781 accagatcat gatggccgac ctcatgatca acggggaaaa gcgtcacgtg gtgttgaccg
     9841 ctcctaagaa tggcttcttc tacattctgg acgccaaaac tggcaaactt ctttccgcca
     9901 agcggctcgt caaaaccacc tgggctactt cttacgacct tcaaacggga cgcccagtgc
     9961 tgatccccgc gtcgcagggc ggcggcaggc agtggacggt ccacaactgg tggcccatga
    10021 gttatagcgt gctcacagga ctcgtctaca ttccatcaac agaccgccgc tccgacacaa
    10081 aagctgccgt agaagcagga gaaggcggcg aggggctctt cggccggctg atcgcatggg
    10141 accctattgc ggaaaccgcg cgctggtccg tcgaagagca gatttccgtc aatggcggtg
    10201 tcttctccac ggccggcaac ctggtttttc agggacaagg taccggcgaa tttgcggcct
    10261 atgccgccga cactgggaaa aaactctggt ccatccagac caagtccgcg atcgattcca
    10321 tccccgtcac tttttcagtg aacggcgaac agtacgtgat tacatccgtg ggctggggtt
    10381 ccggatctcg cctctttgct cccgcgcgca ccatggccac acccgaatcc aagcgcggcc
    10441 caaaccgcct gtatgccttc aaactcggcg gcaaagttcc atttccggct ccgcacattg
    10501 aagttccgcc cgtgccgaaa ccgcctgagc aaaaagtgag cgaggaaatc attcgcaagg
    10561 gcgaaaaact ttacgtgtcg ttttactgcg aaggttgcca ctctcccttc atcgacggca
    10621 gcggcgcttg ggtggaggac ggcgcgatcc ccgatttgcg ctacgctccg tctgaagtgc
    10681 ataaggattg gtatggcatc gtcttgggcg gcagccactg gtcgaaaggc atgcccgggc
    10741 ttgcggatcc gccgaagttt gcttttccca aagctcatat gacggtcgct gaggcggatg
    10801 cggttcacgc ctacgtgatc agccaggcgt ggaaggcgta taaaggtgaa caggaaattg
    10861 cccacagcaa gcgcgaaaat tactagctcg cctttgcgct ggcaaaaccg gccgccaggc
    10921 tgagcaaccc aaccacgagg tggatgaaat tgaaatgcgt gttcagatcc agttggatgg
    10981 gacccagatc gcgcagaccg gcgaagcccg cgatcgccac cagtgtatag atcgctccaa
    11041 aaatcaggcc aaacagccgg ggtccatccg ctctctttcc gagcccggcc caaagggcga
    11101 ttacgccgct gacgagatga aaaacattat gggcgggagg gaatgtaagt ttgaacattt
    11161 cgtggcgaaa gaatcccact actccgacca cgagcaagat cgctccgctc agcacgacat
    11221 agatttttgc caatgtgctt cccatttctc cctgccccct cctattcgcg cgcgattcaa
    11281 tcgcggaatt tcgtggagct tacggcgcgg gcggatgcgc agtcaagtcc accccggacg
    11341 gcgcagggaa gcccggcgag ctgcacgcct tcggttgagc cgatcggcaa ggtaagcata
    11401 gcgaggaacc gaaaaatctt ggccgttcga aaaaggagaa gttttaaggc tataatgcgc
    11461 gccaatcgtg aacccatccg cgtggaacag aattggtcct cgatgtctcc gctgacggtc
    11521 agcgatttgc cattccgtac gaagccgggc aacccaccgc ggccatcacc ctggtcgtca
    11581 attggcccgc cgagctgaaa atgaaatgag gctcctcgtg cttggatcca taagggatgg
    11641 aaaatgaaaa caactgaacc gaacccgaat acccgcggct ggtggaccag tattctcagc
    11701 gatattcatt tttgggtgcc cgtcgccgtc ttgataggtg gactgcttct tcttcgattt
    11761 attcgctgat ctctagatcg gagcaacatg ccgcccactt cgccttccca gtttcccccc
    11821 gcaatcaaag acgcgtcgcg ttcgaaaaag cttcaactgc tgggcattct ttgcggcttc
    11881 gctgcgggag cctggctcgg cgccgcggaa gcgcccacga aactcgtttc cattggcatc
    11941 tcaccgatgg tcatttcact catcatggtc atcggcgttt ttctcgcgcg ctggagcctg
    12001 cccgccctga tcctgggcac ctcgtcggtc cgcgccgatg ttcgccgcgc gccgcacctg
    12061 attatttggg cgcttcttgc cggatgtctt tgggccgttg ccaacactct gacgattttc
    12121 gccatccgtg acatcggctt gagcatcgcc tttcccttgt ggaattcgaa tagccttctc
    12181 ggcattcttt gggggtttct ttttttcaac gagctgcgtc aagccggctg gcggcgtctc
    12241 gccggtgtct tgggcggcgc gcttgtcatg tgcgcgggcg cggcgttctt ggccgtcgca
    12301 tcgtcgtcgc gcggccccgc cgcgcattcc ctgcatggtg tctgggccgc gctcggcgcc
    12361 ggaattcttt ggggcaccat gtacatcccc taccgcaagg cctacctgac cgggatgaat
    12421 ccgctttcct ttgtgacttt cttttcgttt ggcgaattgg gcatgatgac ggtcctcgcc
    12481 gtgagctccc tgggtctcgc ttcgttgtgg caggaattgt tgagcgcgcg cagcgtcatt
    12541 ttctggctga tgctgggagg cttcgtctgg gtgattggcg acgtcttcca gcagtacgcc
    12601 gcaaagtacg tgggcatcag ccggggcatc ccgctttcca attccaatca gctctggggc
    12661 ttgctctggg ggattcttgt attcggagag ttgcatggcc gcagttcttc catctacctc
    12721 gaagtcatcg gcggctccct cctcatgatg ctgggcgtct gcgccatcgc gttttcttcc
    12781 gccaccggtc aggagcagac ctgctggaaa gaagcggcca ttcgggaaag cgaccgctac
    12841 ggtgtcgccg cagacttcgt cgaggcccgc atggacggcc gccaactcct tggtgaatca
    12901 aaaccttccc gcagcgcgct ggactggttc ttggttgtcg ccgccaccag tgtgttcgtg
    12961 atctttgagg ccatggctcg cgtcccgcgg atgtccttgc actgggtgcc agccgcgctt
    13021 ttgactgcgc tccttttctt gttcctcgct gtttgcggcc tcgccctctg gcgcaccacg
    13081 cgcttcaact gaacgggcct tccagccttt gaaaacttcc ccgcttcctt tagagtggcc
    13141 gggacgatca tgacgcaatc agcaaagctc gatgttgtcg gcgtgggact caacgccacc
    13201 gacactctta ttcctgtcgc acattatccc gaacgtgggt ctaaggtcga attccgctcc
    13261 gcgaaagtgc tgcccggcgg ccaggtcgcc accgccatga ttgcctgcca gcagtggggc
    13321 ttgcgcacgc gttacgtcgg caaggttgga gacgacagcg ccgcatcgct ccatcgcgcg
    13381 gaatttgcgc gtctcggcgt cgaggcgcat cttttcaccg cacccgggtg tcccagccag
    13441 caggcgttca tccttgttga tgactccggc gagcgcaccg ttctctggaa acgcgacgac
    13501 cgcctgaccc tgcacccgga agaactccaa cgcgattgga tcgtaaacgc ccgcgctctt
    13561 cacgtcgatg gacatgatac ggctgccgcg actgcagccg cgggctgggc tcgcgccgcc
    13621 ggaattcctg tcgtcgccga cgtggatgat ctttaccccg gcgtggaaac gctcttgaag
    13681 agcgtcgact acctcatcac cagccgagac attccgggtc gcctcatgga agaacccgat
    13741 ctgaaaaaag ccctgcccgc cctcctgaaa cgctttggct cccgactcac cgctgcaaca
    13801 ctgggcgaag aaggtgtatt ggcttgggac gggacgcaat tccactacgc cgctgccttt
    13861 cacgtgcagc cggttgacac cactggcgca ggagatatct ttcacgccgg cttcatctac
    13921 gggctcttgc agggttggcc cttgcaccgc cagctcgatt tcgcctgcgc cgccgccgcg
    13981 ctcaactgca ccgccatcgg cgcccgcggc ggcatccagt ccatcgaagt catcgagcat
    14041 ctcatcgcca ccggcgttcg acacgctcag gcttacgaaa tatcaggttt gacttgaacg
    14101 aatttcccgg gtgttcgaat accgcagcaa tgcatcgctg gtgagagggg gattcccgac
    14161 agtattcggc ggcgtcttgg gcggtggcca gttttcggct acgcgactta tcgggcactt
    14221 gaaaccgagg aggcctgcaa gtaagcgcaa cctcactcgg tggtgtaaac gcctgcttgc
    14281 ttcatggcga caatatgaag caggctggtg agaccgtact tattgtgaaa ttccattgtg
    14341 cggactgagg gtcggggaag tcggtcagag tgttctgatg tcggacggtc agggtagtcg
    14401 aatatccggc gattcgccgg aagcctggct gctcgtgcgc gcaaatttaa gagcgaacag
    14461 gatgacgaga ttccagaaca ggagcgcggc cgcaatccaa agcagaatcg aggagatctg
    14521 ctcttgattt ttctgaatga ggtaggagcg ctccttgaca atttctccgt aagactttcc
    14581 gcagccacgg tagatgacag agacgaatcc aaggaaggct gggagaaaga gtccggcggg
    14641 aaacatcgct tttagtattc gagataggtt gcctgtcgac agtgtttggg tgattccccc
    14701 gaaggatcgg cgcagataga gccaggttgc gccggcgaac gcgatggcca gcgccgccac
    14761 tgcaaagtac gagacgatca ggtaccggtc atttgtcatc tttgatcttc agcgtggcga
    14821 ggatgaccgt agtcaagaca agatacccgc cgagatagag aagcatctgc tcaaggacgt
    14881 tcgataagcc ggaagctact ttccaaacgt attccatgaa gtgggaaccc ggcttgaggt
    14941 cgaaagtaaa tccaagcgct cctcccaggg ccgagaagaa gagcaggaca aagaacgagc
    15001 ggagatagaa agttacgccg tcaggcagat gcacagtgcg ttgcagcagc ccccgcaggg
    15061 cgtcccgcaa tgggagaaag aggccaatag cgagcgcggc cgacaggacg aagagtgcta
    15121 taaacgcata tgtattggcg cccatgtttt tggctcctcc cgaccacgag ttttcgggac
    15181 agcgacacct tgctttttct ggacgggcaa tgtagcgggc cgacaacctc acttatatca
    15241 cagctttggg ccgcgaatct ggcagtgcgg ttagtggaag tgcaacgcgc taatcggaaa
    15301 ctgaggtgat tcgagataac tgtcgaaaac atcctttatc tccgtctatc cacccaaggc
    15361 ttgcgtcact accactggtg gagttcgatc cacggcccgc gcacgcctta ggttgagccc
    15421 ccgccgactc gggtgttatc ctgcctccta tggcggatcc cgtcatctcc ccgccggaag
    15481 tggcgttcac ctccccgctt gaacattccc ggcgtcagct cgaagccttg ctcgaagtca
    15541 gcgaggccat cgcccagcag cgcgatctcc ccgcgctgtt ccacgacctt gccggccgcc
    15601 tccattccgt tgtcgacttt gactttctca ccctcgttct tcacgacgct tcacgcaacg
    15661 tcatgcgtct ccacgttctt gaaacgcgca tccccggtga aaagcacacc ggcaccgaga
    15721 ctcccgtcga gggccatccc tccggctggg tctggcagac gcagcaggcc tttgtcgtca
    15781 gcgacaccga agaggagacg cgcttccccg atttcatgca gcgcctccgc gaatataacg
    15841 tccgctccct cgccatcatc ccgttgacga cggcgcaacg ccgcctcggc gccatgggat
    15901 tcggccgcat cgttcctcag ggaattaccg acaccgagct ccagtttatg cagcgtgttg
    15961 ccagtcaggt cgccgtcgca gtcgataacg ccctcaatct cgaaacttcg caggcttacc
    16021 agcagcaact cgcgcgcgag cgcgatcgcc tccgggtact tctcgaagtc aacaatgtcc
    16081 tcatcaccag ccgcgaactc cccgaacttt tccgcggcat cgtaaacact ctggaacgtg
    16141 tcatccacca cgactacacc agcctcgcgc tcctcgattc ccaaaccggg ctgctcaaga
    16201 ttcacgcgct cgattttcct ggcaaccaga gcctcttcaa acaggagatc accatctcgc
    16261 gcgatgtctc gccctccggc cacgccatat ccaccggcca gccgcttctc gcccgcggcg
    16321 cggaactgga ccgctacacc agtgaaatca tccgcgtcct ccgcagtgag ggcattcaaa
    16381 ccgtctgctg cgttcctctc gtcacccacg gtcacacctt cggttcgctc aatctcgcca
    16441 gccgccgcct cgacgcgttc accctgcaag acgtagaact cctccagcag gtctgcgcgc
    16501 aagttgccat cgcggtcgag aatgctctcg ctttcaagga aatcgatgtc ctgaaaaaca
    16561 agctcgccga agaaaagctc tacctcgagg aagaaattcg cagtgaattc aattttgaag
    16621 aaatcatcgg cgagagtccc gcgctcaagc gcgctctggc tcaggtcgag ctggccgccc
    16681 ccgccggcac taccgtcctc ctcctcggcg aaaccggcac cggcaaagag cttttcgccc
    16741 gcgccatcca taatctcagt ccgcgccgcg atcgcacctt cgtcaaaatc aattgcgccg
    16801 ccattcccag tggtctgctc gaatccgagc tcttcggcca cgagcgcggc gcgttcaccg
    16861 gcgctatcaa tcaaaaaatc ggccgcttcg aactcgccga tcgcggcacg ctctttctcg
    16921 acgaagtcgg cgaccttccc ctcgaacttc agccgaaact tcttcgcgtg cttcaagaac
    16981 aggaattcga acggctcggc ggcaatcgca cacagcgcgt ggacgtccgt gtcgtcgccg
    17041 ccacgaatca ggacctttcg aaacgcgtcg ccgagcgcgc ctttcgcagc gacctctatt
    17101 accgcctgaa tgttttcccc attcagattc ccgctctccg cgagcgcgcc gaagatctcc
    17161 ccctcctcgt tcgctacttc gtgcagagat tcagccgcca gctcaacaaa ggcgtcgagt
    17221 acattcccgc cgacgccatg gacgccctca ttcactattc ctggcccggc aacgtgcgcg
    17281 agctcgagaa cctcatcgaa cgcgccgttc tgctctctcc tggcaaagag ctgcgcatcc
    17341 ccttcgccga attgaaatcc gcggccctcg cgaccgccgc aggtgccgaa ccctcttcgt
    17401 cctttacttc ctttgcctcc ttaacgtcct ttaccccctc ctccatctcc accctcgaag
    17461 aagccgaacg ccaacacatc ctccgcgcct tgaagcagac gcagtggcgc atcgccggct
    17521 ccaagggcgc cgccgtcctc ctcggcatga agcgcaccac cctccaggcc cgcatgcgca
    17581 agcttggtat ccgccgctcc gtttaagcct tcgcagcgga tgcttcctgt tttcggtttt
    17641 cccgtggaat tgtctacttc agttcattgt ttatcaaatt tgccaagcgt gcgccagcca
    17701 gcgcgatccg ctgcttggcc agagtcttgg ccttgctctt gtacccgctc gtcagcgaaa
    17761 agggcccgtc cccggccgcg accggcggtt gataaaccgt ctgttgcgcg gcctgaaaac
    17821 tttctgcaat ccagtctttt tcatcggact tcgccgcttg tccggcatcc gccgccggca
    17881 atttcttcgc ggctttgatc accgcttgcg cgcttgtccc cttgccgaac aggtcgtccc
    17941 agaacgcgtg cagctccttg gcgcatcccg agcaattcaa tttcactttg ttcccgccgt
    18001 tgtcgccgcc gggatcggca ctgctcacgc gtgtcgcgct gtgcagcggc tggtgtacgt
    18061 cacccaccaa gtgcagcagc cacgtcaaat cgtaagactt cagcttgtcg tcgctgtttg
    18121 acgccagtac tccgcggaag agcgcgatcc gttcctgcgc gttcggcgtc ggaattgccg
    18181 gcaacgtcga tccgtcgttt gtgaacggtg tgtccacaaa atgaaagtac ttgtgcttca
    18241 gcagatctct gtagccggtg ttttggctgg acgacacccc gtccggagtg tttccgccgt
    18301 tgcttccgtc gtcgctgtag gcgctatccc ctttgatctc gtccgcccac tgcgccgcca
    18361 gcatgaacac catcatgtct ttgtccgccg gcgacgcgct agccgggacc caactcatcc
    18421 attcgttgaa tttcggattt tgactcagca gatccttcgc ccgcgctttc gcttgcggcg
    18481 tcagcttctg gtatgccaca taggccaccg ccatgtgccc gtaggtattc catccatacg
    18541 ccggcgcacc gagtccccct gccaacacta ccgtcgctat caccagaaga attctcttgg
    18601 tcatacttca cctccatccg agctttgtgt gcatttcctt acgcgtctgc aactacgaac
    18661 aggaaaaaag ccggtcccag agccccttga gacaccactg tcaactagtg ctctcgctcc
    18721 cggattcgtt tcatccctcc gcccggtctt acacctttct ctagctacag cctactgctt
    18781 tattttcctt gcttctcgcg cttggcgctt ccgcccggcc tgcactggta attgtttgcg
    18841 caaaggtgga ttcggtggcg catctcccgg tgtctttctt tgctagccta cagatacgaa
    18901 acgacataac ccccagcggg gcgtgtcgct gccttgtatt gttcatcgtg gccccaccgc
    18961 ggcctcggaa tgttttcgcc ttgaatggcg atcgggagtg tgacgtgaaa atcgcatcgg
    19021 tatttctcgc ctttaccgga tcgttcggca tgctggctgc tcccgcgctc ggtcaggcgc
    19081 ccccgcccgc caaccccgct aaaagtgttt cgcgcaccac caaagccgtg aattaccgcc
    19141 gcgccggtgg ctccaccaag atcgattttc aaggctccga attgatgcac caggccagcg
    19201 gcgaagccaa ggtgcagaac aaaggaaacc gcacggagat tgatgcaaaa ttcgtaggcc
    19261 tggatgaagc caccaagttt ggcctcgaat atctgaccta cgttctctgg gccgtctctc
    19321 ctggggggcg cgccgtaaac ctcggtgaag ttgtgctgaa gaatggcagt ggtgaagtga
    19381 aagccatctc cgacatgcag accttcggca tgatcgttac tgccgaacca tatttcgccg
    19441 tcactcagcc cggcaacacc gtcgtgctgg aaaacgtttt cggtcccgcc actctcggca
    19501 aggtcgaaaa tatcgatgcc agctacgaac ttctcggccg cggcatctac tcttcttcca
    19561 acaccaaaat cgaaaacgcc atctttggca tcgaccgcaa aactccgctc gaactttttg
    19621 aagcgcgcaa ctccgtgcgc attgcgcaca tcgccctggc cgacaagtat gccgcttcca
    19681 cgctcgccaa agccgagcag cagctcaaga gtgcggaaga agtttacgcg cgcaagagcg
    19741 acaagaaatc cgtcattgcc gccgcccgcg aagtcgtgca aaccgcggaa gaagctcgcg
    19801 tcatggccgt aaaactcaaa gccgaagagg acgcccaggc caaagccgcc gccgagaaga
    19861 aatccgcgga gcaacgagaa gccaaggccc gcgctgatgc ggaagccgag gccaggcggc
    19921 gtcaggaagc tgagcaagct cgggtgcaag cggaagcggc tcaagccgaa gccgtacgca
    19981 tgaagcagca agccgagaaa gccgcgcagg aggcggcgcg ccagcaacag gaagcggaac
    20041 gccaaaagca ggaagccgaa caagcaagag ctgcggccca ggcacagcag caggccgctg
    20101 tcgaacagca gcacgccgca gaggccgaaa cggaaaaagc gcgtcaggcc gcagcacagg
    20161 ccgaggcgga aaaggttcag cttcgcgcgc aactcctcaa ccagttgaat tccatcttgc
    20221 agacgcgcga ctccgcgcgc ggcctcatcg tcaatatgtc ggatgtcctg ttcgacactg
    20281 gcagctccac gctcaagccc ggcgctcgcg aaaagttggc caagatctcc ggaatccttc
    20341 tcgcgcatcc gggactcact ctccagatcg agggccacac cgacag
//
LOCUS       DASYVO010000142         2171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_88518, whole genome shotgun sequence.
ACCESSION   DASYVO010000142 DASYVO010000000
VERSION     DASYVO010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88518"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VGR03_04775"
     CDS             complement(<1..728)
                     /locus_tag="VGR03_04775"
                     /inference="COORDINATES: protein motif:HMM:NF013112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV1993623.1"
                     /translation="MTKVGFVSLGCPKNLVDSEVMMGILARGGYELTPRADEAEVLVV
                     NTCSFIEAAQKESVDAILEMAEHKKFGAAKKLIVAGCLVERFRAQILEQVPEVDAIVG
                     TGEIERILEAIEGDLRVLPAQSPAFLYHDLTPRIVTTPKHAAYIKIAEGCDHPCTFCI
                     IPQLRGTFRSRRFESVVREAENLAGAGAREITLIGQDTTSYGEDLGLRDGLAQLLEKL
                     AQVDDLLWVRFLYAYPNRVTQKLL"
     gene            complement(795..>2171)
                     /locus_tag="VGR03_04780"
     CDS             complement(795..>2171)
                     /locus_tag="VGR03_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEV1993624.1"
                     /translation="WTSNEHTPQTLLPNPDGWIYTAEPSSKTDSPGVGLLYTVFLEPL
                     ATDAIFVPGKVISLKGNFTGEGGNSFSVMRRTYILRDSTGTLLNPFHNYSAIRYSGFS
                     RLPSLNAAKLRAASTIYSEEITSTYLQLPPQLDRRIPEFAMQITKNAKTPFDQALAVE
                     NYLRTRFAYTLILTGKPGDDPLAHFLFDTRAGHCEYFASAMTIMLRTLGIPSREVNGF
                     LPGEYNDLGGDYIVRASDAHSWVEVYFPGDGWQTFDPTPAAPESATSFLTRLGKYADW
                     LEITWSEWVIGYDFTHQLVLAQNLQRSSRNWSETARGWFEQKQQNGKKWMRSLQFKHS
                     ALGFLLPVSLVLFLVTLRFNLIAELIRRIRLFFQLHASKSARTNPQLASRLYSELLRV
                     LARRGLLRMESQTHFEFAAAVDSPNLAPAVREFTHIYAHARFGGAPCDTSRLRQLLDQ
                     IRGIQRSR"
BASE COUNT          469 a          590 c          669 g          443 t
ORIGIN      
        1 tccagcagtt tctgcgtcac gcgattcgga taggcgtaaa gaaagcgcac ccacagcaag
       61 tcgtcaactt gcgcgagttt ttccaacagc tgcgcgagcc catcgcgcag gcccaggtct
      121 tcgccgtagg aagtggtgtc ctggccgatg agcgtgattt cgcgcgcacc agcgccggcg
      181 agattttccg cttcgcggac taccgattcg aatcggcggc tccgaaacgt gcctctgagc
      241 tgcggaataa tgcagaatgt gcagggatga tcgcagcctt cggcgatctt gatgtacgcg
      301 gcatgcttcg gggtggtgac gatgcgcggc gtcaagtcgt gatagaggaa cgcaggcgac
      361 tgtgcaggaa gcacgcgcag atccccttcg atcgcttcca ggatgcgctc gatttcgccg
      421 gtgccgacga tcgcgtcgac ttccggtacc tgctccagaa tctgcgcgcg aaagcgttcg
      481 accaggcagc ccgcgacaat tagttttttt gccgccccga atttcttgtg ctccgccatt
      541 tccagaatgg cgtccaccga ttccttttgc gcggcctcga tgaaactgca cgtattgacg
      601 accagcactt cggcttcgtc ggcgcgcggc gtcaactcat agcctccgcg cgcaagaatc
      661 cccatcatca cctcactgtc gacgaggttc ttcggacatc ccaggctgac gaatccgacc
      721 ttggtcatag gcagtaaatt tacaccttta cgtgattttc aattgtaaca cgggcctcgc
      781 cggaatccgg ggcttcaacg ggagcgctgg atgccgcgaa tttggtcgag gagctggcgc
      841 aggcgggacg tatcgcaggg cgcgccgccg aaacgcgcgt gagcgtaaat atgcgtgaat
      901 tcccgcacgg caggagcgag attcggcgaa tcaactgcgg cggcaaattc gaagtgagtt
      961 tgcgactcca tgcgcaggag accccgccgg gcgagcactc gcagcagctc agagtacagc
     1021 cgtgaagcga gctgaggatt tgtgcgcgcc gatttcgacg cgtgcaactg gaagaacaaa
     1081 cggatgcggc ggatcagctc tgcaatgagg ttgaaacgca acgtgacgag gaaaagcacg
     1141 agcgaaaccg gcagcaaaaa gcccagggca ctatgcttaa actgcaagga cctcatccat
     1201 ttcttcccgt tttgctgttt ttgctcgaac cagccgcgtg ccgtttcgct ccaattccgc
     1261 gaactgcgtt ggaggttctg cgccaggact aactgatgcg tgaagtcgta gccgattacc
     1321 cattcgctcc acgtgatctc cagccagtcc gcgtacttcc ccaagcgcgt gaggaagctt
     1381 gtcgcggact cgggagcggc gggggttgga tcgaacgtct gccagccatc gccgggaaag
     1441 tacacttcga cccaactgtg cgcatcgctt gcgcggacga tgtaatcacc gccgaggtcg
     1501 ttgtattcgc ccggaagaaa accgttcact tcacgcgaag gaattccaag tgtgcgcagc
     1561 atgatggtca tcgccgaggc gaagtattcg caatggcccg cgcgcgtatc gaaaagaaaa
     1621 tgggcgagcg gatcgtcgcc gggttttcca gtcaaaatca gcgtgtaggc gaaacgagtg
     1681 cgcaggtagt tttcgaccgc cagggcctga tcgaagggag tcttcgcgtt cttggtaatc
     1741 tgcattgcga attcgggaat acggcgatcg agctgcggcg gaagttggag atacgttgaa
     1801 gtgatctctt cggaatagat cgttgaagcg gcgcggagct ttgctgcatt gagcgagggc
     1861 aggcgcgaga aaccggagta acggatggcc gagtagttgt gaaagggatt caagagtgtg
     1921 ccggtggaat cgcggaggat atatgtccgc cgcattacgg agaaagaatt tccgccctcg
     1981 ccggtgaaat ttcccttgag ggaaatgact ttgccgggaa cgaaaattgc atcggtggcc
     2041 agaggttcaa ggaaaaccgt atagagaagt cccaccccgg gcgagtccgt cttcgaactg
     2101 ggctccgcag tgtagatcca gccgtcggga ttgggcagca gagtttgcgg ggtgtgctcg
     2161 ttggaagtcc a
//
LOCUS       DASYVO010000143         3829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_88585, whole genome shotgun sequence.
ACCESSION   DASYVO010000143 DASYVO010000000
VERSION     DASYVO010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88585"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1962
                     /locus_tag="VGR03_04785"
     CDS             <1..1962
                     /locus_tag="VGR03_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEV1993625.1"
                     /translation="FLEAKVEQQTEDNYKGKEGDLFIRFVIQEGKQTRVASLAIEGNH
                     AFKEEELLGTIASTSGQPYSDFNVTTDRDNILALYFNEGFPEARFNATAESVSAGPAA
                     QKADAGGSASSSQENGKKEQKERESKPAFEQAEAVRLMYHIQEGPQTRVRRILISGYE
                     HTRLGVIHREVHIKVKEPLREGDVVESQRRLYNLGVFNRVTIEPQNPNGTNPEKDVAV
                     LVEEAKRYTVAYGGGFEVQRLASTTSPTGAQIQAAPRGILEVSKLNLTGRGDSLSFKL
                     RGSTLQGRALLGYSAPNTFANPHFSFQATAFAEKTRDINTFTEERYEGSVQLTDQVTQ
                     LTTVLYRYAFRRVHVSNLKILSEEVPLFNQPTLVSQFGMTWFRDSRDNPADASKGSFN
                     SADFSDADTKIGSSASFLRLFFQNSTYYPIKRRFSFARSVRLGILVPYRDTVSLSFPA
                     PSCPTGTSTCSLPTVIPLPERFFAGGGTSLRGFALNQAGPRDAATGFPVGGQALLVFN
                     QEFRFPMRLPFFGASLGGAIFYDGGNVYSRLSRISFRSTLPAPVFRAASLQAIPPETP
                     CPSTHPLCKTDFTNELNYFAHTIGLGVRYKTPVGPIRVDFGYQLNRPSFAIPIPCPSN
                     ATNCQIGSLGQQSTRLPGFQIFFNLGSSF"
     gene            1962..2612
                     /locus_tag="VGR03_04790"
     CDS             1962..2612
                     /locus_tag="VGR03_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993626.1"
                     /translation="MRGLAIRVFLVSLLMANTAGMLASQETVDRIAARVENDIILLSD
                     IRALSRYQQFLDGKSETDAQILDRLIDQWMVRTEADVSRFPRPSDAEIDGSLERLRKS
                     FTSAEQYEARKKQSRLSDSEVRGIVASQLYLSSYLDSRFRPAVQVDSKAIEDFYQNAL
                     VPQAKARGQEPPALDTAHDLIQEALVQRGINEQAEKWLKESRVRVHIENLLGGGSK"
     gene            2609..>3829
                     /locus_tag="VGR03_04795"
     CDS             2609..>3829
                     /locus_tag="VGR03_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993627.1"
                     /translation="MKRETRHPIWSWIIHTPWVLAVIGVLSVIAFFGSGAGNPLLSRL
                     LVSRLERMTGGKVELRALSIRWFAMRATIKGMVIHGKEPAGTEPLFTAEEVQAGLRID
                     SFWGRRISLDELVVQQPHVHIRVEKNGTTNVPAPPRPVSINKPWRDTLFELHIRRLLL
                     SDGWVLYNDIKTPITVHGGDLRLGFDVGGTLDHPLYLGNLDWQNMQFTSKPYMPLRVG
                     LSAKFTLWRDGFTLEQGVLSAGHSHLDAQAEMNSFSNPQWSFRYRGWVDLLDFRETLR
                     EPMVPTGRVDVRGEGQFAGGHFKGSGSYSGQEITLPYEIFHARGLTSHGSYRIDNVGL
                     EVPDFFAGAFGGRVTGRVSMRFDGLKFRADTHVENVKLAGVFPAIEHRDFPIDELHWD
                     ARLTADTVETWSGAF"
BASE COUNT          830 a         1075 c         1130 g          794 t
ORIGIN      
        1 tttctcgagg ccaaggtcga gcaacagacc gaagacaact acaaaggtaa agagggcgat
       61 ttgtttatcc gcttcgtgat tcaggaagga aagcagacgc gcgtcgcttc gctcgcgatc
      121 gaggggaatc atgccttcaa agaggaggaa cttcttggca ctatcgcctc gacttcgggg
      181 cagccgtatt ccgatttcaa cgtgacgacc gacagggaca acattctggc actttatttt
      241 aacgagggat ttcccgaggc gcgcttcaat gcaacggcgg aaagcgtctc cgccggtcca
      301 gcggcacaaa aagcggacgc cggcgggagc gccagttctt cgcaggaaaa tgggaaaaaa
      361 gagcagaagg aaagagagtc caagccggca tttgaacagg ccgaggcggt tcggctcatg
      421 tatcacatcc aagaaggccc gcaaacgcgc gtgcggcgca ttctaatcag cggctacgag
      481 cacacgcgac tgggagtgat ccatcgagag gtgcacatca aagtcaaaga accgctccgc
      541 gaaggggacg tcgtggaatc gcaaaggcgt ctgtacaatc taggtgtctt caatcgtgtc
      601 accatcgaac cgcagaatcc caacggaacg aatcctgaga aggacgtcgc cgttctcgtc
      661 gaggaagcca agcgctacac ggtggcctac ggcggcggat tcgaagtgca gaggctggca
      721 agcacgacga gcccgacggg ggcacagatc caggccgctc ctcgcggaat tcttgaggtc
      781 agcaagctga atcttaccgg gcggggggac tcgctttcct tcaaactgcg tggcagcacc
      841 ttgcaggggc gcgcattgct ggggtattcg gcgccgaata ctttcgctaa tccacacttc
      901 agctttcaag caacggcgtt cgcggaaaaa acacgcgaca tcaatacgtt tacggaagaa
      961 cgctacgagg gttcggtgca gctgacggat caagtcacgc agctgacgac ggtgctctat
     1021 cgctacgcct tccgccgagt tcatgtctct aatctcaaaa tccttagcga ggaagttccg
     1081 ctcttcaacc agccaacact ggtttcacaa ttcggaatga cctggttccg ggactcgcgc
     1141 gacaatcccg ccgacgcttc gaaaggcagt tttaacagcg ccgacttcag cgacgcggac
     1201 acgaagattg gatcgagcgc gagctttttg cgtttgttct tccagaattc gacctactat
     1261 ccgattaaaa ggcgctttag cttcgcgcgt tcggttcgcc taggcatact tgtgccgtat
     1321 cgcgatacgg tctctttgag cttcccggcc cccagctgcc cgacgggaac cagcacctgc
     1381 tctctaccaa ctgtcatccc actcccggag cgattcttcg ccggtggtgg cacttcgctg
     1441 cggggttttg cactgaacca ggcgggaccg cgcgatgccg caacgggttt tcctgtaggc
     1501 ggccaggcgc tgctcgtgtt caaccaggaa ttccgtttcc cgatgcgttt gccgtttttc
     1561 ggggcgtctc ttgggggcgc catcttctat gacggcggca acgtgtacag ccgcctgagc
     1621 cgcatttcct ttcgatcaac gctacctgca ccggtgttca gggcggcaag tctccaagca
     1681 attcccccgg aaacgccatg tccctcgaca catccgctgt gcaagacgga cttcaccaac
     1741 gaactgaact actttgctca caccatcggt ttgggcgtga gatataagac cccggtgggt
     1801 ccgattcgcg tcgatttcgg ctatcagctc aatcgcccgt cgtttgctat tccgattccg
     1861 tgtccgagca atgccacgaa ttgccaaatt ggctcgctgg gccagcaaag cacgcgactc
     1921 cccggctttc agatcttttt caatctgggg tcgagcttct agtgcgcggg ctggcgattc
     1981 gcgtttttct ggtctcgctg ctgatggcga atacggcggg aatgctggcc tcgcaggaaa
     2041 ccgtggaccg tattgcggcg cgggttgaaa acgacatcat tctattaagc gacatccgcg
     2101 cgctcagccg ctaccagcaa ttccttgacg gaaaatcgga aactgacgcc cagattctcg
     2161 atcggttgat cgatcagtgg atggtacgaa ccgaggccga cgtatcgcgt ttcccgcgtc
     2221 catccgatgc cgagattgac ggcagcctcg agcgtctgcg gaagtcgttc acctcggcgg
     2281 agcaatacga agcaaggaaa aagcaaagca ggctgagcga ttcggaagtg cggggaatcg
     2341 tagcttcgca actgtacctg agcagctacc tggactcgcg ctttcgcccc gcagtgcaag
     2401 tcgattccaa ggcgattgaa gatttttatc aaaatgcact cgtgccgcaa gccaaagcgc
     2461 gcgggcaaga gcctccagcg ctggataccg cgcacgacct catccaggaa gcgctcgtgc
     2521 agcgcggaat caacgaacag gctgagaagt ggctgaagga gagcagggtg cgcgtgcaca
     2581 tcgagaatct tctgggtgga gggtcgaaat gaagcgggag actcgccacc ccatctggag
     2641 ttggatcata cacacgccct gggttctggc agtcatcggg gtgttgtcgg ttatcgcgtt
     2701 ttttgggagt ggcgcgggca acccgttgtt gagccggctg ctcgtaagcc gtttggagcg
     2761 gatgaccggc ggtaaggttg agttgcgcgc tctgtccatt cgatggtttg ccatgcgggc
     2821 aaccataaag ggtatggtga tccacgggaa agagcccgcg ggcacggaac ctctttttac
     2881 cgcggaggaa gtacaggcgg gtctgcgcat cgattccttc tggggccgca gaatttcgct
     2941 ggatgagctc gtggtccagc agccacacgt gcacatccgc gtcgaaaaga acggcaccac
     3001 aaacgtgccc gcacctcctc ggcctgtttc cataaacaaa ccgtggcgcg acacgctctt
     3061 cgaattacac atccggcgcc ttctgctctc tgatgggtgg gttctctaca acgacataaa
     3121 gacaccgatc acagtacatg gaggagatct gcggcttggg tttgatgtcg gcggcacgct
     3181 ggaccatcct ttgtatctcg gcaaccttga ctggcagaat atgcagttca catcgaagcc
     3241 ctacatgccc ttgcgagtgg gtctatcggc gaaattcacg ctttggcgcg atggcttcac
     3301 gctggagcaa ggggtactca gcgcggggca ctcgcatctc gatgcgcaag cggaaatgaa
     3361 tagtttctcg aatccacagt ggagtttccg gtaccgcggc tgggtcgatc tgctggattt
     3421 cagggaaact ctgcgcgaac cgatggtgcc gacggggcgg gtggatgtac gcggagaagg
     3481 ccagtttgcc ggcggccact tcaaggggag cgggagttat tccgggcagg aaatcacctt
     3541 accgtacgag atctttcatg cgagaggcct caccagccac ggcagctatc gcatcgacaa
     3601 cgttggactg gaagtgccgg attttttcgc tggcgccttt ggcggcagag tgaccgggcg
     3661 cgtgtcgatg cgtttcgacg gattaaaatt ccgcgcggac acacatgtgg aaaacgtcaa
     3721 gctggcgggc gtttttccgg ccatcgagca tcgcgatttc ccgatcgacg aactgcactg
     3781 ggatgcccgc cttacggcag acacggtgga aacctggagc ggcgcattc
//
LOCUS       DASYVO010000144         3722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_172836, whole genome shotgun sequence.
ACCESSION   DASYVO010000144 DASYVO010000000
VERSION     DASYVO010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172836"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1001
                     /locus_tag="VGR03_04800"
     CDS             126..1001
                     /locus_tag="VGR03_04800"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEV1993628.1"
                     /translation="MPGQQTASKTEKEPVEIKLAHSPDSDDAFMFYALATHKLSTPGY
                     KYTHILSDIQSLNEAALTETYDVTAISFAAYPTLRDKYILLDCGASFGEGYGPIVVST
                     KSLKPTELAGKRIAVPGLKTSAYLTLKLYEPDFEPVVMPFDKIIDAVQKDVVEAGLLI
                     HEGQLFFPQMGLHRVLDLGIWWQEQTGLPLPLGGNAVRRALGANLGRQISKTIRDSVS
                     YGLEHREEALNYAMQFAREMDTELADKFVGMYVNKWTLGYGEKGRQAVRELIKRGTKA
                     GLLPGPPTVEFLGQE"
     gene            1112..1954
                     /locus_tag="VGR03_04805"
     CDS             1112..1954
                     /locus_tag="VGR03_04805"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV1993629.1"
                     /translation="MIGQTISHYRILEKLGGSGMGVVYKAEDTRLHRAVALKFLPEEM
                     THDPQALKRFQREAEAASALNHPNICTIHAIGEENSQAFIIMEFLEGNTLKHCIGGKP
                     LALEQVLDLGIQVADGLDAAHREGIIHRDIKPANILVSKRGHAKILDFGLAKLVPARS
                     AEEAANFSSMPTVSEQDMLTRPGAAIGTVAYMSPEQVRGEELDARSDLFSFGVVLYEM
                     VTGVRPFRGDTSGVLTEAILNRVPVAPVRLHPNAPAKLEEIINKALEKKSQVALSERG
                     GAPH"
     gene            1920..>3722
                     /locus_tag="VGR03_04810"
     CDS             1920..>3722
                     /locus_tag="VGR03_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993630.1"
                     /translation="MRYQSAAELRTDLQRLGRDAESSRSAAAIAVKPRWLALVGAAVV
                     IVGVAIGGWQFYAHRAPALTEKDTIVLADFANTTGDAVFDGTLRQGLTVQLEQSPFLS
                     LVSEQRIQHMLRLMGQSSDARLTPEIARDLCQRMESAAVLDGSIASLGSQYVLGLKAM
                     NCRTGDSLAQEQVTADGKERVLKALGEAAVKLRGKLGESLTTAQKFDTPVEQASTPSL
                     EALQAYSLGWKTRVGKGDDAAAVPLLQRAIRLDSNFAMAYAALGTSYFNLGESSLGAE
                     NTKKAYELRERVSEREKFYIESHYFSTVTGDLEKARRAYELWAQTYPRDWVPPDNLGG
                     IYRILGQYDKRLVENRETLRLDASGVSYAYLVYPYLNLNRLEEARAVAEEAQAKKFDS
                     PLLRGALYRLAFLKNDSAGMAQQVAWSAGKPGVEDLLLAYEADTAAFSGRLGKAREYT
                     RRAVASAEGAEERETAAGYEVEAALREALFGNATKAQQWAAAALALSEGRDVQYGAAV
                     ALALAGDASRAQTLADDLGKQFPEDTVVQFNYLPTIHAQLALSRNDSSKAIEALQAAA
                     PYELGSPISGGAFSPALYPVYVRGEAYLAGHQGSE"
BASE COUNT          769 a         1133 c         1137 g          683 t
ORIGIN      
        1 cctctccgct gcaggaaagt ctctctggga agaaccaaag ccagggcgag gcagccgttt
       61 cgcggttgca cctgaaatga tccgaccttg ccggagtcgc accggaaaag gaaacgaaca
      121 cgcccatgcc tggccaacaa acagcttcga agaccgagaa agagccggtc gagatcaagc
      181 tggcacattc accggattcc gatgatgcct tcatgtttta tgccctcgcc actcataagc
      241 tctccacgcc cggttacaag tacacgcaca tcctctcgga tatccagtcg ctgaacgagg
      301 ccgcgctcac cgaaacgtac gacgtcaccg ccatcagctt tgccgcctac cccactctgc
      361 gcgacaaata catcctgctc gattgcggtg ccagttttgg cgagggctat ggccctatcg
      421 tcgtttccac caaatcccta aagccgactg agcttgcggg caagcgcatc gccgtgcccg
      481 ggctgaagac ctcggcttat ctcaccctca aactctacga gccggatttt gaacccgtgg
      541 ttatgccctt cgacaagatc atcgacgccg ttcagaagga tgtggtcgag gctggcctgc
      601 tcattcacga agggcagctc ttcttccccc agatggggtt acaccgggta ctcgacctgg
      661 gtatctggtg gcaggaacag actggcttgc ccctcccact gggcggcaat gcggtgcgcc
      721 gcgcgctggg ggcgaacctc ggccggcaaa tctcgaagac gatccgcgac agcgtttcct
      781 acggcctcga gcaccgcgaa gaagccctga actatgccat gcaattcgcc cgcgagatgg
      841 acaccgaatt agccgacaag tttgtcggta tgtacgtgaa caaatggacg ctcggctacg
      901 gcgaaaaagg caggcaggcc gtaagagagc tgatcaagcg cggcaccaag gctggcctgc
      961 tcccgggccc gccgacagtc gaattcctcg gccaagaata gtcgccgcaa ttcgcctgag
     1021 aattctacct aacccactac cgaggcccgc ctttgattga cttgtccctc ggcgcggcct
     1081 aacatgcgtc gccaatggta gtttcctagc tatgattggc cagaccatct cccactaccg
     1141 catcctggag aagctcgggg gcagcgggat gggtgtcgtg tacaaggccg aggacacccg
     1201 gctgcatcgc gccgtcgctc tgaaattcct gccggaagag atgacgcatg acccgcaggc
     1261 gctgaaacga ttccagcgcg aggcggaagc tgcttccgca ctcaatcatc cgaatatttg
     1321 cacgattcat gccatcggcg aagaaaacag ccaggcgttc atcatcatgg aatttctgga
     1381 aggcaataca ctgaagcact gcatcggcgg taagccgctg gccttagaac aggtgctgga
     1441 cctggggatt caggttgcgg acgggctcga tgcagcccac cgagaaggca ttatccatcg
     1501 cgacatcaaa ccggctaaca tccttgtcag caagcgtggc cacgcaaaaa ttctggattt
     1561 cgggctcgcc aagctcgtgc ccgcgcgcag tgcggaagaa gccgcgaact tttcgtcgat
     1621 gcccaccgtc agcgagcaag acatgctcac acggcccggg gcggcgattg gcactgttgc
     1681 atatatgtcg ccggagcagg tgcgcggcga agaactcgat gcgcgcagcg acctgttttc
     1741 gtttggcgtc gtgctctacg aaatggtcac cggggttcgg cctttccgcg gcgacacctc
     1801 aggcgtgctt accgaagcga ttctgaatcg cgtgccagtc gcgccggtgc gcttgcatcc
     1861 aaatgcgcct gcgaagctgg aagaaatcat caacaaggcc ctcgaaaaaa aatcgcaagt
     1921 tgcgctatca gagcgcggcg gagctccgca ctgacctgca acgcctcggt cgcgacgcgg
     1981 aatcgagccg ctcagcggcc gctattgccg tgaaaccgcg ttggctagcc ttagtgggtg
     2041 ccgccgtggt gattgtcggc gtcgccatcg gcggctggca gttctacgcg catcgcgcgc
     2101 ccgccctgac cgagaaggac acgattgtcc tcgccgactt cgcgaacacc accggcgatg
     2161 cagtcttcga cggcacgctc cggcaggggc tgacggtgca gcttgaacaa tcaccctttc
     2221 tcagccttgt ctccgaacag cgcattcagc acatgctgcg gctgatgggc cagtcgtccg
     2281 atgcgcggct cactccggag atcgcccggg atctttgcca aaggatggag agcgctgcgg
     2341 tcctcgatgg ctcgattgcc agcctgggca gccaatatgt tctgggcctc aaggcgatga
     2401 actgccgcac gggcgattcc ttggcgcagg agcaggtgac agcggacggc aaggaacgag
     2461 tcctgaaggc actcggtgag gcggccgtga agctgcgcgg gaagctgggg gaatcgctca
     2521 ctacggcgca gaagttcgat acgccggttg agcaggccag tacgccctcg ctcgaagccc
     2581 ttcaagccta cagcctggga tggaagacca gggtgggaaa aggcgatgac gccgccgccg
     2641 tgcccttgtt gcagcgggcc attcgcctcg actccaattt cgccatggcc tatgccgcgc
     2701 tcggaacgag ctatttcaat ctcggggaat ccagcctggg agcggagaac acgaagaagg
     2761 cctacgagct gcgcgagcgg gtgagcgagc gggagaagtt ctacatcgag tcgcactact
     2821 tttctactgt caccggcgac ctggaaaagg cgcgccgggc ctatgaactc tgggcacaga
     2881 cctatccgcg agactgggtg ccgccggaca acctgggtgg catctacaga attctcgggc
     2941 aatatgacaa acgccttgta gaaaatcgcg agaccctccg cctcgatgcg agtggggtga
     3001 gttatgccta cctcgtctac ccctatctca atctgaatcg cctggaagag gcccgggccg
     3061 tcgccgaaga ggctcaggcg aaaaagttcg attctcctct tctgcgcggc gccctgtacc
     3121 ggcttgcttt cttgaagaac gattcggcag ggatggcgca gcaggtggca tggtccgcgg
     3181 gcaagccggg agtggaagac ttgctgctgg cctacgaagc cgataccgcc gccttttccg
     3241 ggcggctggg gaaggcccgg gagtataccc gccgggcggt ggcctcggcc gagggggcgg
     3301 aagagaggga gacggcggca ggttacgaag tcgaagcggc cctgagagaa gccctgtttg
     3361 gcaatgcaac caaggcccag cagtgggccg ctgcagccct cgccctttcg gagggccggg
     3421 atgtgcagta tggagcggcg gtggcgctgg ccttggcagg ggatgcgtcc cgggctcaaa
     3481 cgctggcgga tgatttgggc aagcagttcc ccgaggatac ggtggtgcag ttcaactacc
     3541 tgccaacgat tcacgctcag ctggcgctca gtcgcaacga ttcttcgaag gccatcgagg
     3601 ccctccaggc cgccgctccc tacgagctag ggtcaccgat cagcggtggt gctttctcgc
     3661 ccgctctata tcctgtctat gtacgtggag aagcgtatct ggccgggcat cagggcagcg
     3721 aa
//
LOCUS       DASYVO010000145         3248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_243584, whole genome shotgun sequence.
ACCESSION   DASYVO010000145 DASYVO010000000
VERSION     DASYVO010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243584"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..496)
                     /locus_tag="VGR03_04815"
     CDS             complement(302..496)
                     /locus_tag="VGR03_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993631.1"
                     /translation="MFFTGDRKVDLADACLIRLAVQLETADILTLDKDFEIYRGGRNK
                     LFRMLLSLKYFPSMTLQDSQ"
     gene            603..1085
                     /locus_tag="VGR03_04820"
     CDS             603..1085
                     /locus_tag="VGR03_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993632.1"
                     /translation="MTTLTFVHVVLSLIGIFSGFIVMFGLFAAKRLDGWTAIFLVTTV
                     ATSVTGFLFPFHRFLPSYAIGIVSLLVLTVAILARYSFHLAGGWRRSYVVTAMIALYL
                     NVFVLVAQAFLKVPALKAMAPTQSAPPFLVSQLIVMAIFVVFTIFAAIKFRNEATGAA
                     "
     gene            complement(1185..2117)
                     /locus_tag="VGR03_04825"
     CDS             complement(1185..2117)
                     /locus_tag="VGR03_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1993633.1"
                     /translation="MNIVDLRQTTVRQIEPLLEEEARHWRDELHWDYRGALELIKRFL
                     DAHALAGCVAFENGVAAGYSFYVLEDQKGLIGGLYVSAKFPQDSIARRLLEEMLYSMR
                     AIPHLARIEAQLMPFSGPVDTPLLDQGFHLYTRQFMLLDLQKLPEEKSGASAGMRLNR
                     WNDRYFEPCAKLIYLAYANHVDGEINDQYRSRSGALKFLKNIILLPGCGQFVPGASFV
                     LHEAGGEELIAAVLTSEVSPGVGHTTQICVLPGYQGHGLGRMLMQTSAEALRSMKFRE
                     LTLTVTSDNRTAVQLYERLGFHTIRSFTAGVWPR"
     gene            2334..>3248
                     /locus_tag="VGR03_04830"
     CDS             2334..>3248
                     /locus_tag="VGR03_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV1993634.1"
                     /translation="MQILFELRYPTKWYTKLLMALLALVFFAALATTAIAGFLVYRIV
                     KPQRSSTEINMASFPGRPEVLEFEVLGGIGKREGWFFPGLRGAPTIILCHGYESSRGE
                     LLTLESALQDHQYNVFIFDFAAHGANAGISTLGYREVDEVRAAVDLLAARTDLDPTRF
                     GLWGYNLGAYAALREAESDKRIRALVLDSVYDQPKQFVKVGVERNGLGGFPFMIRAAE
                     FSFGYLNYAHLEDPPLSRKLITLAGVPTLYIQALDDPELADTTRQMFLKAPEPREQAI
                     IPHGNFVSLNDDDKRTYENRVVSFFLVRL"
BASE COUNT          659 a          852 c         1047 g          690 t
ORIGIN      
        1 gaccatgact atggtgaggc cccctgcccc cccatcgtga gggaaagagt gcgcaattca
       61 atgattctgg gcaagttagc cgaatcttcg aggaacgcga ccacgtcgaa cgatctcaac
      121 acaattgcca gggaaggcgg atgcggatgg cgcagtatgt tccaactaaa atagtaccag
      181 aacagttaag gcaggtcaag gaaaagctgg aggatgtcaa agctctttaa aaatgtcccc
      241 tttcttaact cgattaaaat gtcccctcgg ggggatgaga agggcttggc gctgggggca
      301 atcactggga gtcttgcaat gtcatggatg ggaaatactt caggctcagc aacatgcgaa
      361 atagcttgtt cctgcccccg cgatagatct caaagtcctt gtcgagcgtt agaatgtcgg
      421 cggtttcaag ttgaactgcc aagcgaatca ggcaggcgtc agcgagatcg accttgcggt
      481 ccccggtgaa gaacaagact gacgcccctg gactttccga tggatcagcg tgtgtggtaa
      541 tttctggtgc agcggcacgg caccaaaatc gttgtgaaag gaggcgcatc atgatcttag
      601 gaatgacgac actcaccttc gtacacgtcg tgttgagtct gatcggtatt ttctcgggat
      661 tcatcgtcat gttcgggttg ttcgccgcga aacgcctcga cggctggacg gcgatctttt
      721 tggtgaccac ggtggcgacg agcgtgaccg ggttcctgtt tccgttccac aggtttttgc
      781 cgtcttatgc gattggcatt gtgtcgctgc tggtgctgac ggtggcgatc ctcgcgcgct
      841 actcattcca cctcgctggc ggctggcgcc ggagctacgt cgtcacggcc atgattgcgc
      901 tgtatctcaa tgtgttcgtc ttggtcgcgc aggctttcct gaaggtgccg gcgctgaagg
      961 cgatggcgcc gacgcagtcc gcgccgccat ttttggtctc gcaactgatt gttatggcga
     1021 ttttcgtcgt gttcaccatt tttgcggcga tcaagttccg taacgaggcg accggtgcgg
     1081 cttgatcccc cagtatttct cgcgcgccga gcccggacat ggggagatcg ctccggaagt
     1141 cagattttcg ggagtcgcaa gagaccagcg tagcttgggt cagttcagcg gggccagacg
     1201 ccggcggtga aggatcttat ggtgtggaag ccgagccgtt cgtagagctg gacggcggtg
     1261 cggttatccg atgttacggt cagcgttaat tcgcggaatt tcatggagcg cagcgcttcg
     1321 gcggaagttt gcatgagcat gcggccgagg ccgtgaccct ggtagccagg cagcacgcag
     1381 atttgcgtgg tatggccgac gccgggcgag acttcgctgg ttaggaccgc ggcgatgagt
     1441 tcctcgccgc cggcctcgtg gaggacgaag gatgcgcccg gcacaaactg cccgcagccg
     1501 gggagcaaga tgatgttctt cagaaacttt agcgcgccgg agcggctgcg gtactggtcg
     1561 ttgatctcgc catcgacgtg attcgcgtaa gccagataga tcaacttggc gcaaggctcg
     1621 aaatagcggt cattccagcg gttaaggcgc atgcccgccg aggcgccgga tttctcctcg
     1681 ggcagcttct gcagatcgag cagcatgaac tggcgcgtgt agaggtgaaa cccctggtcg
     1741 agcagcggcg tgtccaccgg gccgctgaag ggcatcaact gcgcttcgat gcgcgcgagg
     1801 tgcgggatgg cgcgcatgga gtagagcatc tcctcgagca ggcgccgggc gatggaatcc
     1861 tggggaaact ttgccgaaac atagaggccg ccgatcaggc ctttctgatc ttcgagcaca
     1921 taaaacgaat acccggcagc cacgccattc tcgaacgcca cgcatccggc cagggcgtgg
     1981 gcgtccagga agcgtttgat gagttcgagc gcgccgcggt agtcccaatg caattcgtcg
     2041 cgccagtgcc gcgcttcttc ttcgagcaac ggctcaatct ggcggacagt ggtctgtcgc
     2101 aaatcgacga tgttcatgga tgggcagaaa gcagcgcggc ccctgccgca cgattccatc
     2161 ataaaggaag gaaagcaggt aaaggaagga aaggaggtga gggaggagac acaacgaaac
     2221 tggctggcgg aaggagcagc ggaaaagatg tgctacgatg caaatcgtcg cgttcatatt
     2281 gcgaggtatg gaaaaagcgg ccagtgccgc cagcccagtt gcgcagacga cgaatgcaga
     2341 tcctgttcga gcttcgctat ccaaccaagt ggtacaccaa gctgctgatg gcgctattgg
     2401 cattggtgtt ttttgccgcg ctggccacta cggccatcgc gggctttctc gtctatcgca
     2461 tcgtgaaacc gcaacgctcc agcaccgaaa tcaacatggc gagtttcccc gggcggcccg
     2521 aggtgttgga gttcgaggtg ctgggaggga ttgggaagcg ggagggctgg ttctttcccg
     2581 ggttgcgagg cgcgccgact attattcttt gccatggcta cgagtccagc cgcggcgagc
     2641 tgttgacgct ggaatcggcg ttgcaggacc accaatacaa cgttttcatt tttgattttg
     2701 cggcgcacgg ggccaacgcg ggaatcagca cgctcggcta tcgcgaggtg gatgaagtgc
     2761 gcgcggcggt ggatctcctg gccgcgcgga cggatttgga cccgacgcga tttgggctgt
     2821 ggggctacaa cctgggcgct tacgcggcgc tgcgcgaggc ggaaagcgac aagcggattc
     2881 gcgcgctggt gctggactcc gtgtatgacc agccgaagca attcgtaaag gtaggcgtgg
     2941 agcgcaacgg tctgggcggt tttccgttca tgatccgcgc ggcggagttc agcttcggat
     3001 acttgaacta cgcgcacctc gaggacccgc cgctttcgcg gaagttgatt acactggcag
     3061 gcgttccgac gctttacatt caggcgctgg acgatccgga gcttgcggac accacgcgac
     3121 agatgtttct gaaggcgccg gagccacgcg agcaggcgat cattccgcat ggtaattttg
     3181 tgagtttgaa cgacgatgat aagcgcacgt atgagaaccg cgtggtgtcg ttttttctag
     3241 tgaggctg
//
LOCUS       DASYVO010000146         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_2530, whole genome shotgun sequence.
ACCESSION   DASYVO010000146 DASYVO010000000
VERSION     DASYVO010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2530"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VGR03_04835"
     CDS             complement(<1..973)
                     /locus_tag="VGR03_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HEV1993635.1"
                     /translation="MKRFISTFAILLAAASLAAAGPRPSNVIANLPGRSATSLNGAWH
                     TIVDPYENGLGGRYFEDKKPANKNNLIEYNFEASPTLSVPGDWNSQRESLLFYEGPLW
                     YERTFSYAKRAGKRLFLYFGAVNYKARVYLNGKQVGEHEGGFTPFNFEITEAVADGRN
                     SLVVEADATRHPEAIPAISSDWWNYGGLTRDVALIEVPEIFIQDYFVQLAKGAPGEVA
                     GWVQLDGAHSARSVTLEIPEIGVRQTIQTDAAGRGDFRFPAKLQLWSPENPKLYRTIL
                     SGAGDRVEDEIGFRTIEARGKQIFLNGKPVFLRGINMHDEAAFRGGRA"
     gene            complement(1198..2508)
                     /locus_tag="VGR03_04840"
     CDS             complement(1198..2508)
                     /locus_tag="VGR03_04840"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1993636.1"
                     /translation="MASTTTNRVDWKLIWAQRGFRYFFFAMFISLFGSGMNFAGVSWY
                     ILTATHSTVKVSGQVIFMTLPGLIVPFLGGVLIDRIDRRYLGVLLDLVRAFFVLGTAY
                     IAWRGHLQLWHLYAMTLITGTGSAMYWATVNALVQEVIPSSQFTGANAAVLVGVQSGM
                     LIAGACVGFLYNTAGIAGILAIDGATYFVSSYCLYRVRRGYVSPRESQKYPREYNEAT
                     EATAEALETGENPEVAEAGLSLAVYADMKEGFAYLRQQPLVRALGITHSIMMAGVVSA
                     NVVLVALANDILRAGPRGLGFLEAGWATGAIIGGLLASQLPQALRMPLYVAACAGLAA
                     GHMATPFVAFLAGAVIMQILFGFCRALGGVVAQSSLMTIVPRHFMGRTQSAMAILTTV
                     VQLFMSFSLGWVAQGAGLVVAYALLAVLYTGATVSAIRARQLLR"
     gene            2602..3210
                     /locus_tag="VGR03_04845"
     CDS             2602..3210
                     /locus_tag="VGR03_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993637.1"
                     /translation="MGNEAQCRVRFGKQQSEGKALLETNEILFRGEFRLKILFSTIKS
                     AKAVDGELRLHTTQGLAIFHLGAAAKKWCEKILHPKSRLEKLGVKPGAKVALLGEFDP
                     EFLQEINRLTKSISSSKVAANPEWVFFAADSKEDLSAVAKFSKSMKGAAALWIVYPKG
                     QKRITENDVLAAGRKAGLKDVKVVGFSATHTALKFVIPLSRR"
BASE COUNT          742 a          981 c          937 g          633 t
ORIGIN      
        1 aggcacgccc gccgcgaaag gcagcctcgt catgcatgtt aatgccgcgc agaaaaacgg
       61 gtttgccgtt caggaaaatc tgtttcccgc gagcctcgat ggtgcggaat ccgatttcgt
      121 cctcaacgcg atcgcccgca ccggaaagaa tcgtgcgata aagtttcggg ttctccggcg
      181 accatagttg aagcttcgca ggaaaacgga aatcgccgcg cccggccgca tcggtctgga
      241 tcgtctgccg gacgccgatt tccggaatct cgagggtcac ggatcgcgcg gagtgtgcgc
      301 cgtcgagctg cacccagccg gcgacctcgc cgggggcgcc ctttgccaac tgcacgaaat
      361 agtcctggat gaaaatctcc ggcacttcga tcagggcaac gtcgcgcgtc agcccgccat
      421 agttccacca atcggagctg attgcgggga tcgcttcggg gtgtcgtgtc gcgtcggctt
      481 ccacgacgag cgaatttcgg ccatccgcga cggcttcggt gatttcaaaa ttgaatggag
      541 taaagccgcc ttcgtgctcg ccgacttgct tgccgttcag atagacgcgc gccttgtaat
      601 tcacggcgcc gaaatacaag aagagccgtt tgccggcgcg tttcgcgtac gaaaaggttc
      661 gctcgtacca cagcggccct tcatagaaaa gcaggctttc gcgttgcgag ttccaatcac
      721 ccgggacgct cagggttggg gaggcttcga agttgtattc gatcagattg ttcttattgg
      781 ccggcttctt gtcctcgaaa tagcggccgc ctaaaccgtt ctcataagga tcgacaatcg
      841 tatgccaggc gccgttcaaa ctggtggcgc ttcgaccggg aagattcgcg attacattcg
      901 agggacgggg cccagcggcg gccaggctcg ccgctgccag cagaattgcg aaagttgata
      961 tgaatcgctt catcgagaca ggcaagaaga taccacttta gcggtcaatc gaatccgaga
     1021 aagaagaagc accaacttat ttcagcccga tcgcctgttc tttgaccgga gattcgatct
     1081 ggttcaagag cgcttagcat ggtgcgctcg cgcacatttc cgaaatattg aagaaagggt
     1141 tgaatcgtgc ggatttccat ggcggtctcg tactaaagaa tgttccctcg ccgaagctta
     1201 tcgcaagagc tgccgcgcgc ggatggcaga gacagtggct ccggtgtaga gcacagccag
     1261 cagcgcatac gccaccacca ggcccgcgcc ctgcgccacc catcccagcg aaaaactcat
     1321 gaaaagctgc accaccgtcg tcagaatcgc catcgcggac tgcgttcgcc ccatgaaatg
     1381 tcgcggcacg atggtcatca acgaagactg cgccaccact ccacccagcg cgcggcagaa
     1441 tccaaacaga atctgcatga tcaccgcgcc ggcgagaaac gcgacgaacg gagtcgccat
     1501 atgacccgct gcgagtccgg cgcacgctgc aacatacagc ggcatgcgca acgcctgcgg
     1561 gagctggctg gcaaggagtc ctccaatgat cgcgccggtc gcccagcctg cctccaaaaa
     1621 gcccagtccc cgcggaccgg cccggagaat atcgtttgcc agcgcaacga gaacgacgtt
     1681 cgcgcttacc actccggcca tcatgatgga atgagtgata cccagcgcgc gcaccagcgg
     1741 ctgctgccgc aggtaggcga agccttcctt catgtcggca taaacggcga gcgagagacc
     1801 cgcctcagcg acctcaggat tttctccggt ttccagtgcc tcggcagtgg cttcggtagc
     1861 ttcgttatac tcccgcggat atttctgact ctcgcggggc gaaacgtacc cgcggcgcac
     1921 tcgatacagg cagtaggaag acacgaaata agttgcgcca tcgatggcca gaatccccgc
     1981 gatcccggcc gtgttgtaaa ggaagccgac gcaagcgccc gcaatgagca tcccgctctg
     2041 cacgccgacc agcaccgccg cattcgcgcc ggtgaactgg ctggagggaa tcacttcctg
     2101 caccagcgcg tttacggtcg cccaatacat cgcggagccg gtgccggtaa tcagcgtcat
     2161 ggcgtagaga tgccagagct gcagatggcc gcgccacgcg atataggccg tgccgagaac
     2221 gaagaaggcg cgtaccaggt cgagaagaac gccgaggtag cgccggtcga ttcgatcgat
     2281 cagcacgccg ccaagaaacg gtacgatcag tcccggcagg gtcataaaga tcacttgccc
     2341 gctgaccttc acggtcgagt gcgttgccgt caggatgtac cagctcacgc ccgcgaagtt
     2401 catcccgctg ccgaagagcg agatgaacat cgcgaaaaag aaatagcgga agccgcgctg
     2461 cgcccagatc agcttccagt ccacccgatt tgtcgtcgtg cttgccatgg aacctactag
     2521 attactatcc taagccgcgc ccgaggtggg actggcgttc ctgcctgtcc caaatccgaa
     2581 cctgtgtcct ggaggaattc catggggaac gaagcccaat gccgggtgcg attcggaaag
     2641 cagcagtcgg aagggaaagc cttgctcgaa acgaacgaga ttcttttccg cggcgagttt
     2701 cggctaaaaa ttctcttctc cacgatcaaa tcggcgaaag ccgtggatgg tgaactccgc
     2761 ttgcatacca cgcaagggct cgcgattttc catcttggcg cggctgccaa aaagtggtgt
     2821 gagaaaattc ttcatcccaa gtcgcgcctg gaaaagctcg gcgtgaagcc aggggcgaaa
     2881 gttgctttgc tgggcgagtt cgatccagaa tttctgcagg aaatcaacag gctcacgaag
     2941 tcgatcagca gtagcaaagt tgccgcgaat ccggaatggg tctttttcgc agcggattcg
     3001 aaggaagacc taagtgccgt ggcgaaattc tcgaaatcaa tgaaaggcgc ggccgctctc
     3061 tggatcgttt atcccaaggg tcaaaaacgc atcaccgaaa acgacgttct cgccgctggc
     3121 cgcaaggcag gcttgaagga cgtgaaagtg gtcggcttct ccgccaccca tacggcactg
     3181 aaatttgtca tcccgctctc tcgacgatag ccactacttt ttgcccagcg aacatctgcc
     3241 tgagtttcgt gtttcgccct tctactttcg cgtctaatat ggccgcatga gcg
//
LOCUS       DASYVO010000147         9534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_58728, whole genome shotgun sequence.
ACCESSION   DASYVO010000147 DASYVO010000000
VERSION     DASYVO010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9534
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58728"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /gene="alr"
                     /locus_tag="VGR03_04850"
     CDS             complement(<1..1018)
                     /gene="alr"
                     /locus_tag="VGR03_04850"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV1993638.1"
                     /translation="MAGTQKSHRKEVRRPVWAEVSLGALTYNLEAIRKYVNPPQEKRK
                     TPRKILSIVKGNGYGHGGPQVAKALEKAGSDWFGVTCTDEGIAVRQAGVRKPILVLTS
                     FVPGEESRLVEHDLTAVIHRCEQLGLLDRAAARRDGKKRVPFHLKIDTGMNRLGIAAG
                     DVECFARQLGKCRNIQLTGIFSHFASSGVFTETFVGQQTREQEERFYEALERLRVLGV
                     DPGIVHLANSAAIVTRPETWADMVRPGAILYGYHPGYDPAEKREEAEARLPLKPVMSL
                     RSRIISVRNVTAGAGVGYDATFVAQRPTRIGVLAAGYGDGIHRSLGNHGNVLLRGKLA
                     PIVGI"
     gene            complement(1733..3100)
                     /gene="dnaB"
                     /locus_tag="VGR03_04855"
     CDS             complement(1733..3100)
                     /gene="dnaB"
                     /locus_tag="VGR03_04855"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV1993639.1"
                     /translation="MPVDTTLEKPLPNNLDAERSVLGAILLDNNALNPAIEHLRPEDF
                     FLDQHRRVFTQMIALGEVQQAIDLVTLTEELNRKGDLEASGGAPYLASLADGMPKVSN
                     IEHYARIVKEKAMLRNLIHATHNIQQRAFEGEDGADTILDNAESSIFALAEDRVKAGL
                     VSIKEIVRDNFERLERIFREGKSITGIPTGYTELDKLTSGLQPSELLILAARPSQGKT
                     ALALNLMENIAIRGGHPVAMFSLEMSKESLLQRLVASVAQIDAHKFRTGHLSKEDWRR
                     MTESLGTISSAPLWLDDAGSISVLEIGAKARRLKRDKGLSMLIVDYLQLITGRGRFGN
                     RQEEVSSISRALKGLAKELQIPVLVLSQLSRAPERDERGPQLSDLRESGAIEQDADVV
                     MFIYRPHFFKAGATPEEREETELRIAKQRNGPTDMVKFVFRSRFTRFEEAAPDAFSQF
                     APDDM"
     gene            3964..4977
                     /locus_tag="VGR03_04860"
     CDS             3964..4977
                     /locus_tag="VGR03_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEV1993640.1"
                     /translation="MAKMKVAQVPKPGADFEIVEREIPNPGAGHVRIRVQACGVCHSD
                     SVTKDGLFPGIQYPRVPGHEVAGVLDDVGSGVTGWKKGQRVGVGWHGGQDGTCIACRR
                     GDFANCLNLKVCGINYDGGYQEYMIAPVEAIAAMPESLDAAEAAPLMCAGITTFNALR
                     HSGALPADLVAVQGVGGLGHLGIQFANKFGYKVAAIGRGPENAALAKKLGAHVYIDSK
                     ATNAAEELQKLGGATVILATAPDSKSMSELVGGLGMNGVLLVVGAGQGPIEASPLQLL
                     FGRRSVKGWSSGIPTDSEDTLRFAELTGVRPMIEKYPLDKAAEGYARMMSGKAEFRVV
                     LTM"
     gene            complement(5054..5500)
                     /gene="rplI"
                     /locus_tag="VGR03_04865"
     CDS             complement(5054..5500)
                     /gene="rplI"
                     /locus_tag="VGR03_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV1993641.1"
                     /translation="MQIILQEDIEKLGHRGDVVTVKPGYARNFLLPQKLAVEATSGNL
                     KAIERIRNSLAKKTATELEAAQKQAALLHGVSLKFTRKTGENDQMFGSVTSADIAEGL
                     AAQGFKIDKRQAQLGEPLKTIGEFPVTIKVFRDVTAEIKVTVEKES"
     gene            complement(5557..6111)
                     /gene="rpsR"
                     /locus_tag="VGR03_04870"
     CDS             complement(5557..6111)
                     /gene="rpsR"
                     /locus_tag="VGR03_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV1993642.1"
                     /translation="MSERVEGMAEEQKQAPAPSGAPRREGGSGGHGGHGPGGGHGGPR
                     REGGPGGGGPGGPGGRPRGKRQYFRKKKVCKFCVEKMDFIDYKRADILSQFVQERGKI
                     LPRRMTGVCARHQRWLGVAIKRARNIALLPFAGSAAGTQAPRVVVPGAPVAVHAAPVA
                     PAAVAPAAPVVAAPAAPVAEAPKA"
     gene            complement(6204..6644)
                     /gene="rpsF"
                     /locus_tag="VGR03_04875"
     CDS             complement(6204..6644)
                     /gene="rpsF"
                     /locus_tag="VGR03_04875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV1993643.1"
                     /translation="MEERYYDLVFIVRPATPEEEIKKVLTVIEHTVAEKGGKIEKTEH
                     WGTRKLAYRVDKHREGIYVHQEIRTTHGELITELERRLRVQDNVIKYQTVRLDEDLKR
                     QKKLTLKREKRAARRPRRTSAPAPAPSAPAAATPAAPEQAAATS"
     gene            complement(6727..7314)
                     /gene="pth"
                     /locus_tag="VGR03_04880"
     CDS             complement(6727..7314)
                     /gene="pth"
                     /locus_tag="VGR03_04880"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV1993644.1"
                     /translation="MRLIVGLGNPDPEYQWTPHNLGFMAVDELANRNGIRVERPEGKA
                     LVGRGKIAGEEVILAKPQTYMNLSGISVRELLGKYELDASGLLVMWDEAQLALGTMRI
                     HPEGSAGSHNGAKSVISSVGTQEFARLRLGCGPDHRLSSRKEYVLRPMKKAELEVAAE
                     MIGEAGDAVEMILTQGIDAAMTKYNRRKPEEPEEE"
     gene            complement(7369..8133)
                     /locus_tag="VGR03_04885"
     CDS             complement(7369..8133)
                     /locus_tag="VGR03_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEV1993645.1"
                     /translation="MPVTTGIQEGKKDMAEKIVVEGSLRATRGKNEARRLRQTGKLPA
                     VLYGGKGEAITLTVNAKQVGAILRSESGHNTLFQVDLGGKHEPAILKDWLVDPVTGSL
                     LHVDLLRVAMDVRMKVKVPVHTFGEPSGVKVQGGVFEVVTREVEIECLPADIPTEFKV
                     DVSGLMLNAQLRASDLPIDKTKMKLLTEPERVLAHVVALKVEEEKPVEVVAAEATTLA
                     EPEVIKKGKKEVEGEEGAEAEAKPKAEKSDKGEKKK"
     gene            complement(8195..9139)
                     /locus_tag="VGR03_04890"
     CDS             complement(8195..9139)
                     /locus_tag="VGR03_04890"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEV1993646.1"
                     /translation="MNDDRFKIFSGTANPVLAESICRHLDVPLGKALLGRFSDGEIYF
                     QILENVRGADVFVVQPCHYPVDNHLLELLLMIDAFKRASAWRITAVLPYYCYARQDRK
                     DKPRVPISAKLVADLLETAGASRALTLDLHAPQIQGYFDVPVDHLYGSPVLVEHFRQL
                     NLPNLTVVSPDAGGVERARFFAKKLDAPLAIVDKRRVDVDVSEVMHLIGDVRGRSALV
                     VDDIIDTAGTLVKTAEALLREGATQVFAACTHAVLSGPAIERIEKSCITEIVATDSVP
                     LSEAAKKMGKIKVLSVADLLARGIRSIHEETSISELFI"
     gene            complement(9350..>9534)
                     /locus_tag="VGR03_04895"
     CDS             complement(9350..>9534)
                     /locus_tag="VGR03_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993647.1"
                     /translation="ELLQRGAAEASLAGSGSAVFGVFPSPAMARRAAVGFPHDQTFVC
                     ETVSRDRYARLMRTAE"
BASE COUNT         1876 a         3094 c         2507 g         2057 t
ORIGIN      
        1 tgatccccac gatcggcgcg agcttcccgc gcaacagaac gttgccgtgg tttcccaacg
       61 agcggtggat tccgtctcca taccccgcgg ccagaacgcc gatgcgcgtg ggccgctggg
      121 ccacaaatgt cgcgtcgtat ccgactccag cgcccgcagt gacgttgcga acgctgataa
      181 tcctcgaacg taggctcatc accggcttca gcggcagccg cgcttcagct tcctcacgct
      241 tttccgcggg atcgtagccc ggatggtagc catagagaat cgctccgggc cgcaccatgt
      301 ccgcccaggt ctccggccgt gtcacgatcg cggcgctgtt cgccaggtgc acgatgcccg
      361 gatcgactcc caggacgcgc agccgctcga gtgcttcgta aaaacgctcc tcttgttcgc
      421 gtgtctgctg cccaacgaag gtttccgtga aaactcccga tgaagcaaag tgcgaaaaga
      481 tgcccgtaag ctgaatgttc ctgcactttc cgagttgcct cgcaaaacac tcgacgtcgc
      541 cggcagcgat gcccagccgg ttcattcccg tatcgatctt caaatgaaat ggtacgcgct
      601 tcttgccgtc gcggcgcgcc gccgcgcgat cgagcagccc caactgttcg cagcgatgga
      661 tcaccgccgt taggtcgtgc tccaccagcc gggattcttc tcccggcacg aaactcgtga
      721 gcaccaggat cggctttcgg accccggctt ggcggaccgc aatgccctcg tccgtgcagg
      781 tgaccccaaa ccaatccgat ccggccttct ccaacgcctt tgccacttgc gggccgccgt
      841 ggccatagcc attgcctttg acgatggaga gaatcttgcg aggcgtcttg cgcttttctt
      901 gcggtggatt tacgtacttg cgaatcgctt ctagattgta tgtcagcgcg ccaagcgaaa
      961 cttccgccca caccgggcgt cgcacttctt tgcggtgact cttctgtgtg cctgccatgg
     1021 gccttccgaa taaaatagtc tacgccattc gcacggcctc agcccgccga cagccgcaca
     1081 gcctgccgcg tgaatgacaa taccctctta cactgcaacc caaaggccct tcccatcccc
     1141 ttaagggaac actctactgc acctaagaaa gggaacatcc tttacgaggc tggacacacc
     1201 taaactcttc tcgctgtttt tacttgacta tcttctcttt ccctggtact ctgaatgtta
     1261 ggtatatttg tcgttaatcc cgtagcctcg gtccagcgtc catccccact gcaaacagac
     1321 aacacacttg cacaattttt gtggaactta agtcccctta gaatcaacac ttacaggggg
     1381 ctatggaagt attcattcta aacaacttaa aaccttttag aatcaatact tatgaaaaaa
     1441 aggggggaag aagcccattt tgcacaattt tggtgcaatg taagtccctt tagaatgaac
     1501 acttgtaaaa gtgtatcaaa acaaaggact ttaacttcct ttagaatcaa cacttatgaa
     1561 aaacatacgg ggtggggggg gtattgttaa ctagacactc gacgccagga tcgccactac
     1621 tacctcctgg tcagttccga acctcgcggc cctccacctc gaagaaacga attccccgag
     1681 ccatcaggtc ggccgcgcaa agtgtggctg cactgccgcg ctgtggcaac aattacatgt
     1741 catcgggggc aaattgcgag aacgcgtccg gcgcagcctc ttcaaaccgc gtgaaccggc
     1801 tgcggaacac gaatttcacc atgtcggtgg gtccatttcg ctgcttggcg atgcgcagct
     1861 ccgtttcttc cctttcttcc ggcgtcgccc ctgccttgaa gaaatgcggc cggtagatga
     1921 acatcaccac gtcggcatcc tgctcgatgg cgcccgactc gcgcagatcc gaaagctgtg
     1981 gcccgcgttc atcgcgctcc ggcgcgcgcg aaagttgact aagcaccagc accggaatct
     2041 gcagttcctt ggccaggccc ttcaaagcgc gagaaatgct ggaaacttct tcctgccggt
     2101 tgccgaagcg ccctcgcccg gtgatgagct gtaaataatc aacgatcagc atggaaagcc
     2161 ctttgtcgcg cttcagccgg cgcgccttgg ctccaatctc caggacgctg atcgaaccgg
     2221 cgtcatcaag ccacaacggc gcggacgaga ttgtgcccag cgattccgtc atgcgtcgcc
     2281 agtcttcttt gctgagatgg ccggtgcgga acttgtgcgc gtcgatctgc gcgacggacg
     2341 ccaccagccg ctgtagcagc gattccttgg acatttccag gctgaacatg gccaccgggt
     2401 gcccgccccg aatagcgatg ttctccatca gattcagcgc cagtgccgtc ttcccctgtg
     2461 aaggacgcgc ggccaggatc agcaactccg agggctgtaa tcctgaagtc agtttgtcga
     2521 gttcggtgta cccagtgggg atacccgtga tgctcttccc ttcacggaag attctttcaa
     2581 gccgttcgaa attgtcgcgg acaatttctt taatcgagac gagaccggcc ttcacgcggt
     2641 cttccgccaa agcaaagatc gaggattcgg cgttatccag aatcgtgtcc gcgccatcct
     2701 cgccctcgaa cgcgcgctgc tgaatattgt gcgtggcatg aatcaggttc cgcagcatcg
     2761 ccttctcttt aacgatgcgc gcgtagtgct cgatgttcga taccttcggc atgccgtccg
     2821 ccagcgacgc caggtacggc gcgccgccgg aggcttccag atcgcccttc cggttcagct
     2881 cctcggtcag tgtcaccagg tcgatggcct gctgcacctc gcccagcgcg atcatctgcg
     2941 tgaagacgcg ccggtgctgg tccagaaaaa aatcctccgg acggagatgc tcgatcgccg
     3001 gattcagcgc gttgttgtcc agcaaaatcg cgccaagaac ggagcgctcc gcatccaggt
     3061 tgttcggtaa cggcttttcg agtgtcgtat caacaggcat ttgtattcgc tttatattcg
     3121 cctacagctg tttttgtagc acaagctcgg ctgcagggca agcacagaaa ttttcattcc
     3181 cgggtacgaa cactaccttg ttcaaagcga tgccgcattg tgtgtggaag tcgtgcgcgc
     3241 gtcaatcggt gtgcatgtgg aatacggaac cccctgttga aaacactcaa tagggttcga
     3301 aattcggaga catgatgttg tgcggatatg caagctgcgg ctggaagcag ttaaggcgac
     3361 ccttgggatt gacgtaaaga tgattaggtt gaggacaggc agccacagag acccgctggt
     3421 gcaacatcga gaatccgcaa ccagtcggac acccatcaaa ttgtagggcc agtaattttc
     3481 cgtccgattt tcggacgata gcacctgcaa cggttgaatc aatcccttgg atgtcgtaca
     3541 caatacgaaa aggccgaccc tccgaatttg ccttgagggc acattgcgtg gctttcgtgg
     3601 catcttcgcg gactcgaacg cggccgcaat tcgtgctgaa taacccggct aaataatggc
     3661 ttctccaatc catcgcgtcc ccagttacgc gaacaagcag aatgctgaat cccatcgaat
     3721 cgagaaacaa caagcaaagt aagagtagaa gcactccgac gatgcggaag atcctacttc
     3781 gcctgggttt gttgtttgtc tgcaagcgtc tgccgcagca atagtaagct ttgctcaccg
     3841 agtcagcaag atgacttaaa taagtgcggg cgcagcaggc tgcgtctcgg catgtgtcgg
     3901 tttgcgcgcg tcgcgcatca aaagttctga tcgtaagtcc agaataaaaa acggagagcc
     3961 tccatggcaa agatgaaagt ggcacaggta ccgaaaccgg gcgcggattt cgaaatcgtc
     4021 gagcgcgaaa ttcccaaccc aggcgccggg cacgtgcgta ttcgcgtgca ggcttgcggc
     4081 gtctgtcaca gcgacagcgt cactaaagac ggactcttcc caggcattca atatcctcgc
     4141 gtgcccggac acgaagtcgc cggagtgctc gacgacgtcg gctctggcgt caccggctgg
     4201 aaaaagggtc agcgcgtcgg cgtcggttgg cacggcggcc aggacggcac ttgcatcgcc
     4261 tgccgccgcg gagattttgc caattgcctc aaccttaaag tttgcggaat caactatgac
     4321 ggcggctatc aggaatacat gatcgccccg gtcgaagcga tcgcggcgat gccggaaagc
     4381 ctcgatgccg cggaagctgc tccgctgatg tgcgcgggaa tcacgacctt caatgcgctc
     4441 cgacacagcg gcgctctgcc cgcggacctc gttgccgtgc aaggcgtcgg cggcctgggg
     4501 catctcggaa ttcagtttgc aaataaattc ggttacaagg tcgcggccat cggccgcgga
     4561 cccgaaaatg ccgcgctcgc caagaaactt ggcgcgcacg tttacattga cagcaaagcc
     4621 accaacgccg cggaagaact tcagaagctc ggcggcgcca ccgtaatcct ggcaaccgct
     4681 ccggactcaa aatccatgtc cgaactcgtc ggcggcctcg gcatgaatgg agtactgctg
     4741 gtcgtcggcg ccggccaagg ccccatcgaa gcttctccgc ttcagttgct ctttgggcga
     4801 aggagcgtca agggttggtc ctccggcatc cccacggatt ccgaagacac gctacgcttc
     4861 gccgagctaa caggcgtgcg ccccatgatc gaaaagtatc cgttggacaa agccgccgaa
     4921 ggctacgcgc gaatgatgag cggcaaagcc gaattccgcg tcgtcctcac aatgtagtct
     4981 gcatatcaaa aaaagcaggg gcgcccgcca cggcgggcgc ccctgttgag atagcttcta
     5041 ccccagcaac ttcctaagat tctttttcga ccgtcacctt gatctccgcg gtgacgtcgc
     5101 ggaacacctt gatggtcacc ggaaattctc cgatggtctt gagcggctcg ccaagctgcg
     5161 cctggcgctt atcaatcttg aagccctgcg ccgccagtcc ttccgcgata tcagcggagg
     5221 taaccgaccc gaacatctga tcgttttctc cggtcttgcg cgtgaacttc agcgaaacgc
     5281 cgtggagcag cgcggcctgc ttctgcgcag cctccagctc cgtggccgtt ttcttggcca
     5341 gggagttgcg aatgcgctcg atagccttta agttgccgga ggttgcctcc accgccagct
     5401 tctgcggcaa aagaaaattg cgtgcatagc ccggcttcac cgtgaccacg tcaccgcggt
     5461 ggcccagctt ttcaatatct tcttgaagaa taatttgcat gagtattctc ttagctcctg
     5521 tttctttgcc ccgaaggggg ttttccctgc ggccctttag gcctttggcg cttcagcaac
     5581 cggagctgcc ggcgcggcaa ctaccggcgc ggccggcgca accgcggcgg gtgctaccgg
     5641 tgcagcatga acagccaccg gcgctcctgg cacaactacg cgcggtgcct gcgtgcccgc
     5701 cgcgctgccc gcaaacggca acagtgcgat gttgcgcgcc cgcttgatgg ccacgcccag
     5761 ccagcgctgg tgcctcgcgc aaacacccgt catccggcga ggcagaatct tcccgcgctc
     5821 ctgcacgaac tgcgacagga tgtccgcgcg cttgtagtcg atgaagtcca tcttctcgac
     5881 gcagaatttg caaaccttct tcttacggaa atactggcgc ttgcctcgtg gccggccacc
     5941 gggtcccccc ggaccaccgc cacccggtcc gccttcgcgc cgcggacctc cgtgtcctcc
     6001 gccgggtccg tggccgccat gaccgccgga gccgccttca cgccgcggag ctccgctggg
     6061 cgctggtgct tgtttctgtt cctctgccat accttccacc ctttcactca tcgctccggc
     6121 ctcgccggag ccaatctgca ggtgagcccg gtcgagccgg agtcaccttt tctggatgca
     6181 aaaaactctt ccggcgcgca gccctagctc gtcgccgcgg cctgctccgg cgctgcgggc
     6241 gttgccgccg ctggggcgga cggcgctgga gcgggagccg acgtgcggcg cggacgccgc
     6301 gcggcgcgct tctcgcgctt cagcgtgagt ttcttctggc gcttcaaatc ttcgtccaga
     6361 cgaacggtct gatacttgat cacgttgtct tgcacgcgca gacggcgctc cagttcggta
     6421 atcagttcac cgtgcgtggt gcggatttcc tggtgaacgt aaatgccttc gcggtgcttg
     6481 tccacgcgat acgccagctt gcgcgtgccc cagtgctcgg tcttctcgat cttgccgcct
     6541 ttttccgcga cagtgtgctc gatgaccgtc agcactttct tgatctcttc ttccggcgtc
     6601 gccggccgca cgatgaaaac caaatcgtag taacgctctt ccatgatgac ctctttttct
     6661 tttgaccccg aacaaatcgg agtcgcttca aacccgtccc gggcgcgcgg cccggcaaat
     6721 tcattcttac tcttcttccg gctcttccgg tttccgccgg ttgtacttgg tcatcgccgc
     6781 gtcgatcccc tgcgtgagaa tcatctccac cgcgtccccg gcttcgccga tcatttccgc
     6841 agctacttcc agctccgcct tcttcatcgg ccgcagcacg tattctttgc ggctgctcaa
     6901 ccgatgatcc ggtccgcatc cgagccgcag ccttgcaaat tcctgtgtgc caaccgagct
     6961 gatcaccgat ttcgcaccgt tatggcttcc cgcgctgccc tcgggatgaa ttcgcatcgt
     7021 tcccagcgcc agttgcgctt cgtcccacat caccagcagg ccgctggcat ccagttcgta
     7081 ctttcccagc agctcccgca cggagatgcc gctgagattc atgtacgtct gcggcttcgc
     7141 caggatcact tcttccccgg caatctttcc ccggcccacc agcgccttgc cctcgggccg
     7201 ctccacgcga atcccgttgc gattcgccag ttcatccacc gccatgaaac ccaggttatg
     7261 cggcgtccac tgatactccg gatcgggatt cccaagtccg acaatgagcc gcatagaccc
     7321 ttatccagaa gccttctaaa cgcaggcccc gtgctgtacg acagcgcgct acttcttctt
     7381 ctcgcccttg tcgctcttct ccgccttggg cttggcttcc gcctcggcgc cttcttcgcc
     7441 ttccacttcc ttcttgccct tcttgatgac ttccggctcg gccagcgttg tcgcttccgc
     7501 tgccacaacc tccacgggtt tctcttcttc caccttcagc gccacgacgt gcgccagcac
     7561 gcgctccggc tcggtgagca gcttcatctt ggtcttgtca attggcagat cgctggcgcg
     7621 caattgcgca ttcagcatca gaccgctcac gtccaccttg aactccgtcg ggatatccgc
     7681 gggcaagcat tcaatttcca cttcgcgcgt caccacttcg aaaactccac cctgcacctt
     7741 cacgccggaa ggctcgccaa aagtgtgcac gggaaccttt accttcatgc gcacgtccat
     7801 ggccacgcgc agcagatcca cgtgcagcag gcttcccgtg acgggatcaa ccagccaatc
     7861 cttcaggatc gcgggctcgt gcttcccgcc aaggtccact tgaaacagcg tgttgtgtcc
     7921 gctctcggag cggagaatcg cgccaacctg cttcgcattc accgtcagcg taatcgcttc
     7981 tcccttgccg ccgtacaata ccgccggcaa tttgcccgtt tgacgcagcc gccgggcctc
     8041 attcttgccg cgtgtcgcgc ggagcgaacc ttcgactaca atcttctctg ccatgtcctt
     8101 cttcccttct tgaattcccg ttgtgacagg cattcttgcc tgtcctcttc ctgccatttc
     8161 tccgcggaca gacaaaaatg tctgtcacac aaaatcaaat aaacagttcg ctgatcgaag
     8221 tctcttcgtg aatcgagcga atcccgcgcg ccagcaaatc cgcaacgctc aaaactttga
     8281 tcttccccat cttcttcgcc gcctcgctca gcggtacgga gtccgttgcc acgatctcag
     8341 tgatgcaaga cttctcaatc cgttcgatgg ccggtccgga gagcaccgcg tgcgtgcacg
     8401 ccgcaaacac ctgcgtcgcg ccttccctca gcagcgcctc cgcggtcttc accagcgttc
     8461 ccgccgtatc aatgatgtcg tccaccacca gcgcgctccg cccgcgcacg tctccgatca
     8521 ggtgcatcac ttcgctcacg tccacgtcca cgcggcgctt atccacgatt gccagcggcg
     8581 catccagctt cttcgcgaaa aatcgcgccc gctccacgcc cccggcatcc ggtgagacca
     8641 ccgtcaagtt cggcaaattc aattgccgga agtgttccac cagcaccggc gagccatata
     8701 agtgatccac cggcacgtcg aaatagccct gaatctgcgg tgcgtgcaaa tccaatgtca
     8761 gtgcccggct cgcgcccgcg gtttccagca agtccgccac cagctttgcg gagatcggaa
     8821 ctcgcggctt gtctttccgg tcctgccgcg catagcagta atacggcagc accgccgtga
     8881 tccgccacgc cgacgcgcgc ttgaaagcgt cgatcatcag cagcagttcc agcaggtggt
     8941 tgtccaccgg gtaatggcac ggctgcacga cgaacacatc cgcgccgcgc acgttttcca
     9001 gaatctggaa atagatctcg ccgtcgctga aacgaccgag caacgcctta cccagcggca
     9061 cgtccaggtg gcggcagata ctctccgcca gcaccggatt cgccgtccct gagaaaatct
     9121 tgaatcggtc gtcgttcacg acgctcgcgc tccctgagca actggaaatc cggaactgtc
     9181 attcttggcc gcggctgaaa gtcgaaagtt aaaggtcgaa agtgaagaag gggtttcggt
     9241 gaatgcagag caatcactgg ccccgtccca atttggtttc aactttaaac tttcgacttt
     9301 taacttctgc gaagagacgc tgaggcagaa tccacgacac gctggcagac tactctgcgg
     9361 tgcgcatcaa acgagcgtac ctgtcgcgcg agacagtctc gcaaacgaag gtctggtcgt
     9421 gcggaaaccc aacagcggct cggcgcgcca tcgctgggct cgggaaaact ccaaacaccg
     9481 ccgaaccgct acccgccagc gaggcttctg cagctcctct ttggagcaac tccc
//
LOCUS       DASYVO010000148         8422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_243722, whole genome shotgun sequence.
ACCESSION   DASYVO010000148 DASYVO010000000
VERSION     DASYVO010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8422
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243722"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VGR03_04900"
     CDS             complement(<1..274)
                     /locus_tag="VGR03_04900"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1993648.1"
                     /translation="MGKERRGKHRSKEGRITGAMDEKELTKQLAQEGFARTNVWQNGA
                     RAHYPDHTHDAETARIILDGEMTLTIGGRSKTFRVGDRCDVPAGLVH"
     gene            complement(379..1254)
                     /locus_tag="VGR03_04905"
     CDS             complement(379..1254)
                     /locus_tag="VGR03_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993649.1"
                     /translation="MNRTRWITGLPAVAVLACAGAAFAQAPPPPAGADGATPHGPRMH
                     ERMRGPGFWNGFELLGFGPGGGPKTVKGAPFSANAVSETTQVLGDGTKIHRSTQGSLF
                     RDGEGRVRWEMNFQGFGPLAASGTSRSAVVIHDPVAGTGYFLEPDGKTARKFTKREAG
                     DHPSKEDGFTHRWERKTQDSDNVTKESLGTQVIEGVKAEGTRITHVIPAGQIGNDKPI
                     SVVTERWFSNELQETVLIKRSDPRFGTSTYKVTNIQRKEPAATLFQVPADYTMKEGHE
                     RNVRRGPAPPPPGSE"
     gene            complement(1321..1992)
                     /locus_tag="VGR03_04910"
     CDS             complement(1321..1992)
                     /locus_tag="VGR03_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993650.1"
                     /translation="MNCEEFAELGFDFERARVSDAERTAAQGHLRICGVCAAQMESWR
                     ELRERLQDVARETQSAQATERVELQLRRKVMLLGYEHRLKRRRTAFAATALAAAAIAV
                     VAFGIRRWGWATTRVATAPAAVSGESGKVVAKQNTGALATPGSEDAANATQEASLTQD
                     EFLRLPGSLPLPEDEGTIVRMRMQRGALGALGLPVNEEQASEWIVVDLLVSADGQPQA
                     VRLSR"
     gene            complement(2010..2621)
                     /locus_tag="VGR03_04915"
     CDS             complement(2010..2621)
                     /locus_tag="VGR03_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1993651.1"
                     /translation="MPREPNAREDDLLRRAVRGDEDAFLQIYRAHQPTLYRYALRMTG
                     RPWTAEEMVQEVFLTLLRESGRFDPSRGALGAFLFGIARNHVRKHLERSPNELQLDES
                     GELPEAGSAVYRVDGNRLANPAVWTEQRQRSEQLRRAILEIPEEFREAIVLCDLEELS
                     YEAAAHLLGCPIGTVRSRLHRGRALLMARMEILRERPRKAAAP"
     gene            complement(2792..3601)
                     /locus_tag="VGR03_04920"
     CDS             complement(2792..3601)
                     /locus_tag="VGR03_04920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1993652.1"
                     /translation="METWRGKWALVTGASAGIGKALAGELAAGGTNLVLTARRRERLA
                     ELAARFSARGVKTEILAADLVDAAAPRAIYNFTKERGVEVELLINNAGFGAYGELRTQ
                     EAGRLAEMIQVNCNAVVQLTRLYLPEMVARRHGDILIVGSTAAFQAVPYISTYAATKA
                     FDLLFAEGVAEEARPYGVRVCCLCPGSTATEFHDVAGQPDSTKRKQETAEKVARTGLR
                     ALAAGKSYVISGMANYLATHGQRLAPRRLVTRVAAKMFRPEEKRETKEIAK"
     gene            complement(3893..3982)
                     /locus_tag="VGR03_04925"
     tRNA            complement(3893..3982)
                     /locus_tag="VGR03_04925"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3946..3948),aa:Ser,seq:gga)
     gene            complement(4224..7088)
                     /locus_tag="VGR03_04930"
     CDS             complement(4224..7088)
                     /locus_tag="VGR03_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV1993653.1"
                     /translation="MKKDYTAARLVISFGALVLILVGLGWFGLSRMDRSNAEFEQVIN
                     KRWNKVRLSREARSYSSVNNRITMEIFLLKSKEEIEPLLIQRAANTEKISVLIKKIEA
                     MGLESAREKELLEAVNAARWPYVNSYIEALHMLLDEHKVEQARTAMVRVTLPRLIIYH
                     NTYNAFVKFQDEQLDAAAAQIRANYASARRLVLFLIVLAVAVAAAIAVQVTRSITREI
                     AGRERAEKKFSDLNEELEQKVASRTAELAEANYDLADETAEREQAEGNYRESNELVRL
                     LLDSTAEAIYGVDLNGNFTLCNAACLRLLGYRDASELRGKNMHEQIHYTRPDGTPYPV
                     QDCLILQAFRDGKGTHIDDEVVWRRDGTSLPVEYWSHPIFRDKKVIGAVVTFVDITER
                     RRAQEELTGAKEAAEAANEAKSTFLATMSHEIRTPMNGILGMTELVLDTDLSVDQREN
                     LGLVRVSAESLLSIIDDVLDFSKIEAGKLDLESIPFDLRESLGETMKALSFRAHQKGL
                     ELICEVEPGVPEAVLGDPGRIRQILVNLVGNAIKFTEHGEIFVKVEEESQGPAASCLK
                     FAVKDTGIGIPVGNQKRIFDAFTQADGSMARKYGGTGLGLTICARLVHMMGGRMWVDS
                     PPGQGSTFFFTVQMSVQNTPTARPIPLQPQQLGDLHALIVDDNFTNRRVLHGMLSRWG
                     MKPTAVDGGRAALQALEVAKSAGHPFPLILLDGQMPEMDGFALAEHIREDPDLVGTTI
                     MMLTSASHLGDAARCRELGISAYLVKPIRQGELLDAICHVLQKGADKKPVPLVTQHAL
                     REEKNRRRVLLAEDNHVNQTLARRLLEKRGYTVSVAGDGRAALQAFEKESFDLVLMDV
                     QMPEMDGFEATAAIRAKEKETGGRVPIIAMTAHALKGDRERCLAAGMDGYVSKPIRTD
                     ELFSAIEEMLRKGMQRNRTSKNPAEPGAAGEAPVPAGQKI"
     gene            complement(7332..7904)
                     /locus_tag="VGR03_04935"
     CDS             complement(7332..7904)
                     /locus_tag="VGR03_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993654.1"
                     /translation="MTKSSLFRAMVASAMVLGFSGAAHAQSESQKPEQPAKEQKPAAK
                     VRKVWTEDDVSSLRSPADVYREKKEAQDEAAAKQAASGQQTLSNKPALASHPPKLSNP
                     KTVDEADGMIAWEKRDVDAQQENLERLQKQLNEAPADQKERLQALIVEHQRILADTRK
                     EMLGLVEQKKGLEKKSSSTNSASSAKPPSP"
     gene            complement(7926..>8422)
                     /locus_tag="VGR03_04940"
     CDS             complement(7926..>8422)
                     /locus_tag="VGR03_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993655.1"
                     /translation="QLLQNIKDNADALIPNPPPAPNVVSHTLYTYGGPGWPAGMSQNN
                     PQVVYVDGDFDLGPNTGYGLLVVTGNFFYHGNSGWKGIVLVVGDGTTTFLGNGGGNGS
                     FDGAVFTATIRDASGNLLNNFGPLNFDVTGGGGNGIYYNSCWIKNVQKPPSYKLLSFR
                     EISQ"
BASE COUNT         1438 a         2828 c         2296 g         1860 t
ORIGIN      
        1 aatgcacgag tccggccggc acgtcgcagc ggtccccgac acgaaacgtt ttactccgcc
       61 cgccgatggt cagcgtcatt tcgccgtcaa gaatgatacg cgccgtctcc gcgtcatgcg
      121 tgtgatccgg atagtgcgcc cgtgccccgt tttgccatac gttcgtgcgt gcgaatccct
      181 cttgcgcgag ctgcttcgtc agttcttttt catccatggc gccggtgatt ctcccctctt
      241 tacttcggtg cttccctctt cgctcttttc ccatacacca acgccctccc cgcggcgagt
      301 gcggcgccgc gagaagggcg ttgtgtcttc aggagagaag ctcgttgttt cggacctccc
      361 tcgccgcaaa acttcgaact actccgaacc aggcggcgga ggcgccggcc cgcggcggac
      421 gttccgttcg tgcccctctt tcatcgtgta gtccgctggc acttggaaca gcgtcgcggc
      481 cggttccttc cgctggatgt tcgtcacctt gtaggtggat gtcccgaacc gggggtcgct
      541 ccgtttgatc aagaccgttt cctggagctc attggaaaac cagcgttccg taaccacgga
      601 gatgggcttg tcgtttccaa tctgtcccgc tggtatcacg tgtgtgatgc gagttccttc
      661 cgctttgact ccttcaatca cttgagtgcc cagcgattct ttcgttacgt tgtcggaatc
      721 ctgcgtcttt ctttcccagc ggtgggtgaa tccgtcctct ttcgatgggt ggtctccggc
      781 ctcccgtttc gtgaacttcc gcgcggtttt cccgtccggt tccaggaaat agccggtacc
      841 ggcgacagga tcgtgaataa cgacggcgga gcggctcgtc ccggaggctg cgagcggtcc
      901 gaacccctgg aagttcatct cccaccgcac gcgcccttcg ccgtcgcgga acaacgaacc
      961 ctgcgtcgag cggtggattt ttgtcccgtc tcccagtacc tgagttgttt ccgaaaccgc
     1021 gttcgccgag aatggcgcgc ccttgactgt cttcggcccg cctcccggtc caaaaccgag
     1081 gagctcaaaa ccattccaga agcccggccc ccgcatccgc tcgtgcatcc gcggcccatg
     1141 cggagtagcc ccgtctgcgc cagcgggggg aggcggcgcc tgcgcgaacg cggcaccggc
     1201 gcacgccagc acggcaacgg ccggcagccc cgtgatccat cttgtacgat tcatccaact
     1261 acctcctctt ctctcccgta acgttggtcc ttcttctcct gtaaagtcct gcgccacgca
     1321 ctaccgcgac agccgcaccg cctgcggctg cccgtccgcg ctgaccaata aatccacgac
     1381 gatccattca ctcgcttgct cttcatttac cggcagtccc agtgcgccca gcgcgccgcg
     1441 ctgcatcctc atccgcacaa tcgtgccctc gtcttccgga agcggcaggc tgccgggcag
     1501 ccgcaggaac tcatcctgcg tcaaggatgc ttcctgagtc gcgttcgcgg catcctcgga
     1561 accaggcgtc gccagggccc ctgtgttttg tttcgcgact acttttcctg attctcccga
     1621 taccgccgcg ggcgcggtcg cgacccgtgt cgtcgcccac ccccatcgcc ggattccgaa
     1681 ggccaccacg gcaatcgccg ctgcggccag cgccgtcgcg gcaaaggcgg tcctgcgccg
     1741 cttcaagcgg tgctcgtagc cgaggagcat tacttttcgg cgcaattgca gctcgacgcg
     1801 ttccgtcgcc tgcgcgctct gcgtctcccg cgccacatcc tgaagccgct ctcgcaattc
     1861 ccgccacgat tccatctgcg ccgcgcacac gccgcaaatc cgcagatgcc cctgggccgc
     1921 ggtccgctcc gcatcactca cgcgcgcacg ttcgaaatcg aaacccagtt cggcaaactc
     1981 ttcgcaattc atccttggtt ctcccggcgt catggtgccg ccgcttttcg aggccgctcc
     2041 cgcaggatct ccatccgcgc catcagcagc gcccgcccgc ggtgcagccg cgagcgcacg
     2101 gtgccgatcg ggcaccccag caggtgtgcc gccgcctcgt aactgagttc ctcgaggtcg
     2161 cacagcacga tcgcctcgcg gaattcctcc ggtatctcca ggatcgcccg ccgcaactgt
     2221 tcgcttcgct ggcgctgttc cgtccacacc gcgggattcg ccaatcggtt cccgtccacc
     2281 cggtagacgg cactcccggc ttcgggaagc tccccgctct catccagctg aagttcgttt
     2341 ggggaacgct ccaggtgttt gcgcacgtga ttccgtgcaa tcccgaaaag aaatgctccg
     2401 agcgccccgc gcgatgggtc gaatcggccg gattcacgca gcagcgtcag aaaaacctcc
     2461 tgtaccatct cctcggctgt ccaggggcgg cccgtcatgc gcagggcata tcggtacagc
     2521 gtcggctggt gcgcccggta aatctgaaga aaggcgtcct cgtctccccg gacggcccgc
     2581 cgcaacagat cgtcctcgcg cgcgttcggc tcccttggca tctctcctgg ttagacgacc
     2641 tgcggggctc catccggtac ttgccgcctg ggggtcaaaa agttcccgga tctcttccgc
     2701 cggacttttt gttgcgggct cttggtaggc gtcctgcgcc ggagccgtct atcccggcgc
     2761 tcgcccatcg aacctatcca actcctcctc attacttcgc tatttccttg gtttctctct
     2821 tctcctccgg ccggaacatc tttgccgcca ctcgcgtcac aagtctccgc ggtgcgagtc
     2881 tctgtccgtg tgttgccaga taattcgcca tccccgaaat cacgtagctt ttccccgccg
     2941 caagcgcccg cagccccgtc cgggccacct tctccgccgt ctcctgtttt cgcttcgtgc
     3001 tgtccggctg gccggccacg tcgtgaaatt ccgtcgccgt cgaacccgga cagaggcagc
     3061 agacgcgcac gccgtacggc ctggcctctt ccgcaacgcc ctccgcaaac agcagatcga
     3121 acgccttcgt cgccgcatag gtcgagatat acggcacggc ctgaaacgcc gccgtggacc
     3181 ccacgatcaa aatgtcgccg tgccgccgcg ccaccatctc cggcaggtac agccgcgtca
     3241 gttgcaccac tgcgttgcag ttcacctgaa tcatctccgc cagccgcccg gcctcttgcg
     3301 tgcgcagctc gccataagct ccgaaacccg cattgttgat gagcaattcg acttcaacgc
     3361 ctctctcctt cgtgaagttg tagatcgccc ggggcgcggc ggcatccacc agatcggccg
     3421 cgagaatctc cgtcttcacg ccccttgccg aaaacctggc cgcaagttcc gccagccgct
     3481 cccgccgccg cgccgtcagc acgaggtttg tcccgcccgc ggccagctct cctgcgagcg
     3541 ctttgcctat ccccgcgctt gccccggtta ccagcgccca tttcccccgc caggtctcca
     3601 tcgttgcccc ctttcgattc cccgaccgcc agagaataac ctcttttcaa tgctcgcgct
     3661 tcccagttga ggccggcatc aaattgagtc gtctagtgcg cgtgatcctg cgcagagcgg
     3721 gagggggagc ccgctgccga attcgtgcct gccctgtgtc ccgcacggcg ggacgaaggg
     3781 agcgaggccg cctcaggcgg ccgagtcgag ggaccttgaa cccaatggcg gagagggtgg
     3841 gaagagttta tcgcgagcgc agccagggga aacccactcg cttctgcgat tctggcggag
     3901 agggtgggat ttgaacccac ggtaggattt ctcctacaca cgctttccaa gcgtgctcct
     3961 taaaccactc ggacacctct ccgcatgtcg tgcaatcctt ataagagtag cagaacccct
     4021 ggccgcgcag aaaatcgccc gccggccaca acacttcctg tccgggctac cgcattccat
     4081 ttacaaaatc gagcagggag tgttcggcgt gggaaactgg atcctcaccc agcctggggc
     4141 cgagtcacat ctttacagtc cacggaagat agccgaggag caatctgcgt tcgaagccgg
     4201 cggcttcggg cgatgccgga gtttcaaatc ttttggccgg ctggcacggg cgcttcaccg
     4261 gcagctcctg gctccgcggg atttttcgaa gtacggtttc gctgcatgcc cttgcgaagc
     4321 atctcttcga ttgcactaaa cagctcgtct gtccggatcg gcttggacac atagccgtcc
     4381 atgcccgcag caaggcagcg ctcccgatct cccttcagcg catgggcagt catcgcgatg
     4441 atggggacgc gcccacctgt ctctttctct ttcgcccgga ttgccgcagt cgcctcgaaa
     4501 ccgtccatct cgggcatctg aacatccatc agtacaaggt cgaaggattc tttctcgaat
     4561 gcctgaaggg cggctcggcc gtcccccgcg accgacactg tgtaaccccg tttctccagg
     4621 agtcgacgcg ccaaggtctg atttacatgg ttgtcctcgg ccaagaggac tcgccttcgg
     4681 tttttctcct ctcgcaaggc gtgttgggtc accaggggaa cgggtttctt gtcggctcct
     4741 ttctgcagaa catgacagat ggcatccaac agttcgccct ggcggatggg tttgaccagg
     4801 taggcggaaa tccccagctc gcggcaacgc gcggcgtcgc ccaaatggct ggcagaagtc
     4861 agcatcatga tcgtggtccc caccaagtcc gggtcctcgc gaatgtgttc ggcaagggcg
     4921 aaaccgtcca tctccggcat ctggccatcc aggagaatca ggggaaatgg atgaccggcg
     4981 ctcttcgcaa cctcgagtgc ctgcagcgct gcccggccac cgtccacggc ggtcggcttc
     5041 attccccagc ggctcagcat tccgtgcaac actcgccggt tcgtgaagtt gtcgtccacg
     5101 atgagcgcgt gcaggtcgcc caactgttgg ggttgaagag gaatggggcg cgccgtggga
     5161 gtgttctgga cgctcatctg gaccgtgaag aaaaacgtgc tgccctgacc gggcggactg
     5221 tccacccaca tgcgcccgcc catcatgtgc acgagcctcg cgcagatcgt cagccccaat
     5281 cccgtcccgc cgtacttgcg cgccatcgag ccgtcagctt gcgtgaacgc gtcgaagatc
     5341 ctcttttgat ttcccacggg aattccgatg cccgtgtctt tcaccgcaaa cttcaggcaa
     5401 ctcgcggccg ggccctggga ttcttcttcg accttgacga agatctcgcc atgttcggta
     5461 aacttgatgg cgttcccgac cagattgacc aggatctggc gaatccgccc gggatcaccc
     5521 agcaccgcct ccggaacacc cggttctact tcgcaaatga gctcgagccc cttctgatgc
     5581 gcgcgaaaac tcagcgcctt catcgtttcc cccaggctct cccgcaggtc aaaggggatc
     5641 gactccagat ccagttttcc agcctcaatc ttcgagaagt ctagaacatc gtcgatgatg
     5701 gaaagcaggg actcggccga gacccgcacc aagcccaggt tctcgcgctg atcgacgctg
     5761 agatccgtgt ccagcaccag ctccgtcatc ccgaggatcc cgttcatggg cgtgcgaatc
     5821 tcatggctca tcgtcgccag gaaggtgctc ttcgcctcgt tggccgcttc ggcagcctcc
     5881 ttggcccctg taagctcctc ttgcgcgcgt ctgcgttcgg tgatatcgac gaaggtcacc
     5941 actgcgccga tcactttctt gtccctgaag atagggtggg accaatactc gaccggaaga
     6001 ctcgttccat ccctgcgcca gacgacttca tcgtcgatgt gcgtcccctt tccgtcccgg
     6061 aaggcctgga gaatgaggca atcttgcacg ggatacggag ttccatccgg ccgcgtgtaa
     6121 tgaatctgct cgtgcatgtt cttaccacgc aactcactcg catcccggta tcccaacaag
     6181 cgcaagcatg ccgcattgca aagcgtgaag ttgccattca ggtcgacacc gtaaatcgcc
     6241 tcggcagtgg aatccagcag gagcctgacc agttcgttgc tctcccgata attcccttcc
     6301 gcttgttcgc gctcggcagt ttcgtccgcc aggtcgtagt tcgcctcggc gagttccgcc
     6361 gtccggctgg ccactttttg ctcaagctcc tcgttcaaat cgctgaattt cttctcggct
     6421 cgctcgcggc cggcgatctc gcgggtgatg ctgcgcgtca cctgcacggc gatggccgcg
     6481 gccacggcga cggccagaac gatcagaaac agaacgagcc tgcgcgcgct cgcataattc
     6541 gcccggatct gcgcggcagc cgcatccaac tgttcgtcct gaaacttcac gaatgcgttg
     6601 taggtgttgt ggtagataat gaggcgcggc agagtcaccc ggaccatggc cgttctcgcc
     6661 tgttcgacct tatgctcgtc caacagcata tggagcgctt ctatgtagct gttgacgtac
     6721 ggccatcggg cggcattcac agcctcgaga agttctttct ccctggcgga ctcaagaccc
     6781 attgcctcga tcttctttat cagcacggaa atcttctcgg tgtttgcggc gcgctggatc
     6841 agcagtggct cgatctcctc cttgctcttc agcaggaaga tttccatcgt gattcgattg
     6901 ttcacgctgg aatagctccg agcctcccgc gaaagccgca ctttgttcca gcgcttgttg
     6961 atcacctgct cgaattcggc attgctgcgg tccatccggc tcaagccgaa ccatcccagg
     7021 ccgaccagga tcaggaccag ggctccaaag gaaatcacca atctcgctgc ggtgtaatct
     7081 tttttcatct gcggtgcttc tcgacgttct cccgccggaa gtctccagga gtaagactgc
     7141 acgtcactag ccatccattc cccgtggctc tgctcaaagc ccttcatttg tatggagttg
     7201 cgccccctgc cagggcgctt ggaccgcatt catgctgaac agtgtaccgt cccgctacac
     7261 ctgttccgaa tggaggggag agatctcaac tcctctgtga ctcggcaaga ttggggtgcc
     7321 gcgctgcagg tttacggtga agggggcttt gcggagcttg cgctgttagt tgaagaagac
     7381 ttcttctcaa gtcctttttt ttgctccaca agtcctagca tttccttgcg cgtatccgcg
     7441 aggattcgct ggtgctcaac aatcagcgct tgcagccgtt ccttttgatc cgccggcgct
     7501 tcgttcagtt gcttctgcaa gcgctcgaga ttttcctgct gggcgtcaac atcgcgcttt
     7561 tcccacgcga tcatcccgtc cgcctcatcg actgtcttgg ggttcgacag ttttggcgga
     7621 tgtgatgcca gcgcgggctt gttgctaagg gtttgctgtc cggaggcggc ctgcttggcg
     7681 gcggcttcgt cctgtgcctc cttcttttca cggtagacat ccgccggcga acgcagtgaa
     7741 cttacatcat cttcggtcca caccttgcgt acttttgctg ccggcttctg ctccttcgct
     7801 ggctgttcgg gtttttgcga ttccgactga gcgtgggcgg cgccggaaaa gccgaggacc
     7861 atggcggatg caaccattgc cctgaaaagt gaactttttg tcatctgttc ttctctctcc
     7921 tcgcgctact gcgagatctc tcggaacgac aacagcttgt acgacggagg cttctgcaca
     7981 tttttgatcc aacagctgtt gtagtagatg ccgttaccgc cgccaccggt aacatcgaaa
     8041 tttaagggtc cgaaattgtt caacagatta ccggaagcgt cccttatcgt ggccgtgaaa
     8101 acggcgccgt caaatgaacc attgcctccg ccatttccaa ggaacgttgt ggtgccgtct
     8161 ccaacgacca ggacaatacc cttccagccg gagtttccgt ggtaaaagaa gttccccgtc
     8221 accaccagca gaccgtaccc ggtgttcggg cccaagtcga agtcaccgtc aacatagacc
     8281 acctggggat tgttctggga cattcccgct ggccagcccg gacccccata agtgtaaaga
     8341 gtgtgactga cgacattcgg agcgggcggc gggttgggga tgagcgcgtc cgcgttgtcc
     8401 ttaatgttct gcaggagttg ac
//
LOCUS       DASYVO010000149         1607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_115765, whole genome shotgun sequence.
ACCESSION   DASYVO010000149 DASYVO010000000
VERSION     DASYVO010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115765"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..1492
                     /locus_tag="VGR03_04945"
     CDS             275..1492
                     /locus_tag="VGR03_04945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1993656.1"
                     /translation="MTGEKQSSPSGARNAILAGFLGWTFDAFDFFILTFVLAAIAREF
                     GQTIPAMALTITASLATRWIGAILFGLFADRYGRRIPLIVNILYFSLIEVLSGLAPNY
                     RVFFFLRLLYGIGMGGEWGVGASLTMESVPARWRGFVSGLLQEGYALGFLLAAAVYRL
                     VYPHWGWRPLFFIGGLPALLTLFVRARVKEPEAWHRSRTDWTTYRRAIFRNWRRFAYL
                     VLLLAMMNFIAHGTQDMYPTFLQQQHGFSPQRTSDFTVISMIGALCGGLMFGFISDGR
                     GRRRTMVTAVLLGIAVIPLWVFAPNLPLILMGGFLMQFMVQGAWGVIPAHINELSPDQ
                     LRGFFPGLAYQFGVLIASGSAYFEAQMAHFMSYSRAMGLFAAVVLLAGALVISLGPEE
                     HCAEFGRRHSAKA"
BASE COUNT          261 a          422 c          501 g          423 t
ORIGIN      
        1 gcagtgcgat gaggatcagc agcacacccg tgatctcgcg tccctggcgt ggcgagagat
       61 gcggcctggt ggtgcgtttg tgccgcgcgg cggggcagaa atcttctcag ttggatcctt
      121 ggttgcgttc tcatttattc cgcccttttc agcatcggta agctctgttt cggcttctat
      181 caagaggggc tgcttcttgc cattctcgcc attgccagtg cgttagctat ctcgcggctg
      241 atgcccaagc cggcggagtg gcaagcaaac taaaatgacg ggtgagaagc aatcctctcc
      301 gtccggtgct cgaaacgcta tcctcgccgg atttctcggt tggacgttcg atgcgttcga
      361 tttcttcata cttacgtttg tgctggctgc cattgccagg gagttcgggc aaacgatccc
      421 agcgatggcc ttgacgatta ccgcgagcct ggctactcgc tggatcggcg ccattttgtt
      481 cggattgttt gccgaccgct acggccgccg aattccgctt atcgtcaata ttttgtactt
      541 ctcgttgatt gaagttttgt ccgggcttgc tccgaattat agggttttct ttttcctgcg
      601 cctgctttac ggcatcggaa tgggcggcga gtggggcgtg ggagcgtcgc tgacgatgga
      661 atccgttccc gcgagatggc ggggattcgt ttcgggcttg ttgcaggagg gttacgcgct
      721 gggatttctg ctggcggctg cggtttatcg cctggtctac ccgcattggg gctggcggcc
      781 attgttcttc atcggaggat tgcccgcgct gcttaccttg ttcgtgcgcg caagggtgaa
      841 agagcccgaa gcctggcacc gctcgcgcac ggattggacg acctatcggc gggccatatt
      901 ccgcaactgg aggcgctttg cctatctcgt tctgttactg gcgatgatga atttcattgc
      961 tcacggcact caggatatgt acccgacctt tttacagcaa caacatgggt tcagtccgca
     1021 gcgcacgtcg gacttcaccg tgatttcgat gatcggcgcg ctttgcggcg ggttgatgtt
     1081 cggatttata tctgacggcc gcggacggcg gcgcacgatg gtgacggcgg tgctgctcgg
     1141 aattgcggtt attccactgt gggtgtttgc ccccaatctc ccgttgattc tgatgggcgg
     1201 attcctgatg caattcatgg tgcagggcgc gtggggagtg attccggccc acatcaacga
     1261 gctctccccc gatcagttgc gaggattttt tccgggattg gcgtaccaat tcggtgtttt
     1321 gatcgcttcc ggcagcgcgt atttcgaagc acaaatggcg catttcatga gctatagccg
     1381 ggccatgggg ctgttcgccg ccgtggtgct cttagccgga gcgctggtaa tctccctcgg
     1441 cccggaagaa cactgcgcgg aatttggcag gcgccattca gcgaaggcgt gacgggcact
     1501 gctttgggtg acgtggcggg agctcgacgc gttgtgttaa tatttatgta cagtgcgccc
     1561 gtagctcagt tggatagagc gtctggctac gaaccagaag gtcggga
//
LOCUS       DASYVO010000150         4102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_131840, whole genome shotgun sequence.
ACCESSION   DASYVO010000150 DASYVO010000000
VERSION     DASYVO010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131840"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2052
                     /gene="priA"
                     /locus_tag="VGR03_04950"
     CDS             <1..2052
                     /gene="priA"
                     /locus_tag="VGR03_04950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV1993657.1"
                     /translation="KGTLLLGPATKLGVDTSALQRLQRRGLIEIREDVRRRKRKTQRV
                     IAWKGGGAAAEKLMAQEGEEQDNAETQSAQRKRREERVQELLETERGPLPLPQLLKLA
                     QVTRAVIERMLRDGLLASLEEPIDPAEDPFDAGYTPPAHELNAEQESALKGIRARFEL
                     GEFGVQLLHGVTGSGKTEVYLRAVQETLARGKTAIILVPEIALTLWMGRQCRAWFGAR
                     FEGVAVLHSALRDVERAREWWRVRNGEARVVVGTRSAVFAPLENVGLIIVDEEQENSY
                     KQEETPRYHGRDVAIVRAKMEGAAALLGSATPSLETYHHARNGKYELLTLASRVAERP
                     LASVEIVDLREEFQQTHQTSPISAALHAGIQECFAKGTQALVLINRRGYSWSVLCRSC
                     GASVQCVNCSISMTYHKHRNRLECHYCGSIQAVPKQCPKCQSKYVYFFGEGSEHLEER
                     LRKEFPGARIARLDRDTARTKRQYQETLGAFAGGALDILVGTQMLAKGHDFQRVTLVG
                     VAGVDSSLSLPDFRAAERTFQLLTQVAGRAGRGELKGRVLIQTFYPEHYAIQDAVKQD
                     YAAFFERELHFRRMMAYPPFTSLANVIVRDTSIEKTIRWSRLLSEYFSPHDGKELRIL
                     GPATAPLARLKKEHRFQFLLKSPKRSVLTKLLSGALAYCDAKEIPQTAVLVDMDPISL
                     L"
     gene            complement(2391..2561)
                     /locus_tag="VGR03_04955"
     CDS             complement(2391..2561)
                     /locus_tag="VGR03_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993658.1"
                     /translation="MKNNFVPGRQGTDDVRRFEFPSDNRLYLIPMEDGQGGVIARKDA
                     AFKLLWERLKQR"
     gene            complement(2558..3892)
                     /locus_tag="VGR03_04960"
     CDS             complement(2558..3892)
                     /locus_tag="VGR03_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV1993659.1"
                     /translation="MPPLLRTLRQRDLLLLFIGSVIGSGIFLTPSLILRQLDGSVGLS
                     LLVWLVGGILSLLGALTYAELAAANPEAGGLYCYIRDGFGRLPAFLYGWCLFLVIASA
                     TIAALARAFTRYLAEIIPLTSTASNLAAILMIAVVTAVNVWGTRKSSDLQNWTTLVKV
                     GIVVLLSAVLLSLGHHAADLPTALGTSQHGFSLFSNFGLAMIAVLWAYEGWQFGTYSA
                     GEVIDPQKCFPRAFLLGSVLLMTLYLVAVIAYLVALGPAAATASDAIAANAVAAVLGS
                     WAGKIVAITILISTFSAMNSVVLTAPRVFYAMANDNLFFKKLAVVHPKFRTPAFAIVA
                     LGIWSAVLSSAGKFAELASGAIFIGWIFYGLGAAAIFPMRRASKGLPIPYRVPGYPWT
                     PLLFVLVAAAIVGNAVYLAFRDPQQFRYLAVALILLLLGLPAYLFWRKRSAS"
BASE COUNT          978 a          979 c         1436 g          709 t
ORIGIN      
        1 aagggaacac tgctcctcgg tccagccaca aagttgggtg ttgatacttc cgctttgcag
       61 cggctgcaac gccgcggact cattgaaatt cgagaggatg tgcggagaag aaaacggaag
      121 acgcagcgag tgatcgcgtg gaaaggcggc ggggcggcgg ctgagaaatt gatggcgcag
      181 gaaggcgaag aacaagataa cgcagagacg cagagcgcgc agaggaagcg cagagaagaa
      241 cgcgtgcagg aattgctgga gacggagcgc gggccgctgc ctttgccgca acttttgaag
      301 ctggcgcagg tgacgcgggc ggtgatcgaa cggatgttgc gtgacgggct gttggcgagc
      361 ttggaggagc cgatcgatcc ggccgaagat ccgtttgacg cggggtacac gccgccggcg
      421 catgaattga atgcggagca ggaaagcgcg ctgaaaggaa ttcgagcacg gtttgagctg
      481 ggtgaattcg gcgtgcaatt gctgcatggc gtgacgggca gcggcaagac cgaagtgtat
      541 ttgcgggcgg tgcaggagac actagcgcgg gggaagaccg cgatcattct cgtaccggag
      601 atcgcgttga cgctctggat ggggcggcaa tgccgagcgt ggttcggcgc gcggttcgag
      661 ggcgtagcag tgctgcattc ggcgctgcgt gacgtggagc gggcgcgcga atggtggcgc
      721 gtgcggaacg gcgaagcgcg cgtcgtggtg gggacgcgtt cggcggtgtt cgcgccgcta
      781 gaaaatgtcg gactcatcat cgtcgatgaa gaacaagaga acagttacaa acaggaggaa
      841 actcccagat atcacggacg cgatgtggcg atcgtccgcg caaagatgga aggcgcggcg
      901 gcgctgctgg gttcggcgac accgtcgctc gagacttacc atcacgcgcg gaacggaaaa
      961 tatgagttgc tgacactggc gtcgcgcgtg gcggagcggc cgctcgcgag cgtggaaatt
     1021 gtggacctgc gcgaggagtt tcagcagact catcagacga gcccgatttc cgccgcgctg
     1081 catgcgggga ttcaggaatg ctttgcgaag ggcacgcagg cactcgtgct aatcaaccgg
     1141 cgcggatact catggtcggt gctgtgccgg agttgcggcg cctcggtgca gtgcgtgaat
     1201 tgcagcattt cgatgacgta tcacaagcac cgcaaccggc tggagtgcca ctattgcggg
     1261 tcgattcaag ctgtgccgaa gcagtgtccg aagtgccaat cgaaatacgt ttattttttc
     1321 ggcgaaggtt cggagcatct ggaagaaaga ctccgcaagg aatttcccgg cgcacgaatc
     1381 gcgcggctgg accgggacac ggcgcgaacc aagcggcaat atcaggagac gctgggcgcg
     1441 ttcgcgggcg gcgcgctgga cattctcgtg ggcacgcaga tgctggcgaa gggacacgat
     1501 tttcagcgcg tgacgctcgt cggagtggcc ggcgtggact cctcgctgag cctgccggat
     1561 tttcgcgcgg cggagcggac gtttcagttg ctgacgcagg tggccgggcg cgcgggtcgc
     1621 ggcgaattga aggggcgcgt tttgattcag acgttttatc ccgaacacta tgcgattcaa
     1681 gatgcggtga aacaggatta cgcggcgttc ttcgagcgcg aattgcattt ccggcgcatg
     1741 atggcgtacc cgccgtttac gtcgctggcg aatgtgatcg tacgggatac gagtattgaa
     1801 aaaacgatcc ggtggtcgcg gctgctttcc gagtattttt ccccgcacga tgggaaagag
     1861 ctgcgcatcc tgggacccgc gacggcgccc ctggcgcgct tgaagaaaga gcacaggttt
     1921 caattcctgt tgaagtctcc gaagcgttcc gtgctgacga agctccttag cggggcgctg
     1981 gcctattgcg acgcgaaaga gataccgcag acagcagttt tagtggatat ggatccgata
     2041 agcttgttgt aatcagtggc ttcgatagga gacttaaagc ttgacttgag gttcggacta
     2101 tatcggggta ccccccccta tttttggcaa agagtgcgca atcgattgaa aagaaggtag
     2161 ttcaattttt caatgtgaca aaagagtgcg caatatcatc aagaaaaagg agttaagccc
     2221 aaaatgaaag cgccgactag taaccaaaat ggcgatagat gggtagagtt aactgcggcg
     2281 gagatgttcc aaggattaat tatagtacag ggtaatcaga aatcaagggg gaaaatgggt
     2341 gtgggtcaga ctgttgtatg ccaagtagtt aggatgttcc accttcccac tcagcgctgc
     2401 ttgaggcgct cccagagcag tttgaaagca gcgtccttgc gggcgatcac gcctccttgg
     2461 ccgtcttcca tggggatgag atagagacgg ttgtcgcttg ggaactcgaa ccgacggacg
     2521 tcgtcggtgc cttggcggcc cggaacaaaa ttgtttttca agaggcggag cgtttccgcc
     2581 agaataaata ggcggggagg ccgagcagta gcaggatgag agcgacggcg agatagcgaa
     2641 attgctgcgg gtcgcggaaa gccaagtaga ccgcattgcc tacgattgca gcagcaacga
     2701 gaacgaagag gagcggggtc cacggatagc cgggaacgcg gtaggggatg ggcagtcctt
     2761 tggaggcgcg gcgcattggg aagatggccg cggcgccgag accatagaaa atccagccga
     2821 tgaaaatggc gccggaggcg agctccgcga atttgccagc gctggagaga acggcagacc
     2881 agataccgag ggccacgatg gcgaaggcgg gcgtacgaaa tttcgggtgg accacagcaa
     2941 gttttttgaa gaagagattg tcgttggcca tggcatagaa aacccgcggc gcagtgagga
     3001 cgacgctgtt catggcgctg aaggtggaga tgaggatggt gatggcgacg attttgccgg
     3061 cccaggagcc gaggacggcg gcgacggcat tggcagcgat cgcgtcgctg gcggtggccg
     3121 cagcgggacc aagagccacg aggtaggcaa tcaccgcgac caggtaaagg gtcatgagta
     3181 gcaccgaacc aagcaagaac gcccgcggga agcacttctg agggtcgatg acttcgccgg
     3241 cactataagt gccgaattgc cacccttcgt atgcccacag aacggcgatc atcgcgagac
     3301 caaaattgga aaaaagggag aagccgtgct ggctggtgcc aagggcagtt ggcagatccg
     3361 ctgcatgatg accaagagag agcaagacgg cactgaggag aacgacgatg ccgactttaa
     3421 ccagcgtagt ccaattctgc agatcggaac tcttgcgtgt gccccaaacg ttgacggcgg
     3481 tgacgacggc aatcattaga atcgcggcaa gattggaagc cgtggaggtg agcggaatga
     3541 tttcggcaag atagcgggtg aaggcgcggg ccagggcagc gatggtcgcg ctggcgatga
     3601 cgagaaagag acaccagccg tacagaaaag ccgggaggcg gccaaagcca tcccggatat
     3661 agcagtacag gccgccggct tcgggattgg cggcggcgag ctcggcgtag gtgagcgcgc
     3721 caagaagaga gaggatgccc ccgacgagcc agacgaggag cgagaggcct acggagccgt
     3781 caagctggcg aaggatgagg ctgggagtga ggaaaatgcc ggagccgatg acggagccga
     3841 taaagagtag gagcagatcg cgctgacgaa gtgtgcgaag aagtggtggc atcgaagggc
     3901 cgagcggcgc actgcgcgcc tgcccgaggg atgcatcata acgggagttt cgggagcgtc
     3961 aacagaaagt tcccaagaac cggccgagtg accaacgcca caggccaatg aatataaaaa
     4021 acgcagatag gggagaagag gcccatgcgc gccggcggga gttacagatt aactaaggat
     4081 tggggcaggc atgaaggaga gg
//
LOCUS       DASYVO010000151        31329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_131875, whole genome shotgun sequence.
ACCESSION   DASYVO010000151 DASYVO010000000
VERSION     DASYVO010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 31329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31329
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131875"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VGR03_04965"
     CDS             <1..879
                     /locus_tag="VGR03_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEV1993660.1"
                     /translation="RRVYASHGTEVKVADADSGAVVGTISGLKQCHGIVLVEELGKGF
                     ISDGGAAKTVIFDMASLKVTGEVKGEEDADSIIYDPASKRVFVFNGDAKSATVIDPAN
                     GTVVKSIPLGGGPEFAVADGKGMVYDNIEDKNEVVAIDSGTLAIKSRWPVAPAGQPTS
                     LAMDREHRRLFAAGRNPQKLVILDADNGKVIQSFPISAGADANVFEPETGLIFVSTRE
                     GMIHIFHEDTPDKYSEVETVKTEFGAKTMALDSKTHNLYLTTADFGPPPAPTAERPHP
                     NPAPIPGTFHLLIYGK"
     gene            1044..1520
                     /locus_tag="VGR03_04970"
     CDS             1044..1520
                     /locus_tag="VGR03_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEV1993661.1"
                     /translation="MQTIVHQLGELFLQAAPTVLIIFLFYIILRAIFFKPLLAVMAER
                     DSRTVGAQKASEDAQAAAAQKVKQYQEALKQARGQLYAEQEAARKKLLEERAAQIKEA
                     RTRAAGEVSAAKERVAAELAAARRDVETTVAQLSAEIARRILQTPPRPGAPASEAR"
     gene            1517..2101
                     /locus_tag="VGR03_04975"
     CDS             1517..2101
                     /locus_tag="VGR03_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993662.1"
                     /translation="MTRNRPLFLAVSILFMLLFAVVGANAAEEGGNAATERATEIFKW
                     INFAIVAGLILWLCLKKAPIFFSGRASAISSAITIATAAKAAADAQVREAETRLANLQ
                     TEVAELRATAEREATAEAARLRAATQTDAQKIAAAARAEIEAAGRAARLELKALAANL
                     AVDGAESLLVKQLTPQAQESLISNFVKSLEGRPN"
     gene            2098..2634
                     /gene="atpH"
                     /locus_tag="VGR03_04980"
     CDS             2098..2634
                     /gene="atpH"
                     /locus_tag="VGR03_04980"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEV1993663.1"
                     /translation="MKSASLQYANAMADIALAQGAAEPAAKQLHEFGAAYAQSAELRT
                     FLASPAVSVEAKHAVIEKIVARLGASKIIRNFLFVIADHRRTQLIPEVIAAFHQVIRQ
                     RQGVAEAEVSSAVELSAAQKKEMAATLARLTGKKIETKYALDPALLGGAVVRIGDTIY
                     DGSLRGRLNEMRARLAAE"
     gene            2709..4244
                     /gene="atpA"
                     /locus_tag="VGR03_04985"
     CDS             2709..4244
                     /gene="atpA"
                     /locus_tag="VGR03_04985"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV1993664.1"
                     /translation="MANIKADEISKILREQIENYEQTVSVDEVGAVISVGDGIARVHG
                     LEKVMAGEMLAFPHNVFGIALNLEEEEVGVVLLGESQELKEGDVVKRTNTIMSVPVGE
                     ALVGRVVNPLGQPVDGKGPIATKQRNPLEKIAPGVLDRQPVREPLQTGIKAIDSMIPI
                     GRGQRELIIGDRQTGKTAVILDTIINQKGGDIICIYCGIGQKRSTIAQVVKTLTDAGA
                     MDYTIVVTSSAAEPASLQYIAPYAACAMGEFFRDTKRHAVTFYDDLSKHAQAYREISL
                     LLRRPPGREAFPGDVFYLHSRLLERAAKLNDKLGGGSLTSLPVIETQAGDVSAYIPTN
                     VISITDGQIYLEADLFNAGIRPAINVGISVSRVGGNAQIKAMRQVAGSLRLDMAQYRE
                     LAAFAQFGSDQLDKVTQGQLARGQRLTEILKQDQYVPLPVEKQVLSLYIATSGLLDAV
                     PVAEVGHFEREFLQFVETNYPSILKNIAAKKALDDAIKSEVKTAVDAFKERFTAAVAA
                     AAK"
     gene            4268..5143
                     /gene="atpG"
                     /locus_tag="VGR03_04990"
     CDS             4268..5143
                     /gene="atpG"
                     /locus_tag="VGR03_04990"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEV1993665.1"
                     /translation="MATLLDFRRRIRSVKNTQQITRAMKFVAAARLRRAQEAAIAARP
                     YAKELARMLRSIMSRIDEPQHPLLAKRSEERILALVLTGERGLAGAFNANILRKAQDF
                     FRVNKGKKISTIPIGKKGRDSLKKAGLGLIAEYVNVLARVDFATAREIALLVTDLYAK
                     EEIDSVYIVFSEFKTVMTPNLVVTKILPVEAVHADEESAAEKEGSQVDYIYEQPVDQL
                     LGKLLPRYIETQILRAMLESSAAEHAARMTAMESATKNAGDVIDALTLHMNKVRQAAI
                     TKEIIEIVSGASYGA"
     gene            5155..6606
                     /gene="atpD"
                     /locus_tag="VGR03_04995"
     CDS             5155..6606
                     /gene="atpD"
                     /locus_tag="VGR03_04995"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV1993666.1"
                     /translation="MAEKYAGVGHVVQVIGPVMDIQFEAGHLPAIFNAVRITSEGYSI
                     PTPLDVTAEVQQHLGEGRVRAIAMEPVEGMVRGMKAYDLGKGIEVPVGRPTLGRVMNV
                     LGKPVDQLGPIVSDTYYPIHRPAPSLEDQSTMLEMFETGIKVVDLIEPYLKGGKIGLF
                     GGAGVGKTVLIMELIHNVAMKHGGVSVFAGVGERTREGNDLWLEMSEGGVIVPGNSEK
                     SRAALIYGQMTEPPGSRLRVGLTGLTVAEYFRDQEHLDVLLFIDNIFRFVQAGSEVSA
                     LLGRMPSAVGYQPNLNTEIGELQERITSTKSGSITSVQAIYVPADDYTDPAPAATFAH
                     LDATTNLSRQIVERGIYPAVDPLASSSRILDPRIVGAEHYAVARGVKSILQRYKDLQD
                     IIAILGIEELSDEDKRTVARARKIEKFLSQPMFVAAQFTGLEGKYVKIEDTVRSFKEI
                     VEGKYDDVPEQAFYMVGTIDEALAKAEKMKASA"
     gene            6615..7052
                     /locus_tag="VGR03_05000"
     CDS             6615..7052
                     /locus_tag="VGR03_05000"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV1993667.1"
                     /translation="MLPDAIELVIVTPERQLLREKAVEVQLPGEGGYLGVLPGHAPLM
                     TELGIGELSYHDLGGKESAHLAIVRGFAEVLSDRVTVLAETAERAEEIDLQRAKDALA
                     RAEKRLASNDPNIDWDRANIALQRALIRIQVATKRRSALVAGS"
     gene            7131..7385
                     /locus_tag="VGR03_05005"
     CDS             7131..7385
                     /locus_tag="VGR03_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEV1993668.1"
                     /translation="METITMYSTTWCPDCRRAKSFLKERGVNFREVNIEEDPSGEAFV
                     IKANNGKRTVPTIEVGGRYFACSPFDAEQLAEELNIPLNP"
     gene            complement(7382..8548)
                     /gene="nagA"
                     /locus_tag="VGR03_05010"
     CDS             complement(7382..8548)
                     /gene="nagA"
                     /locus_tag="VGR03_05010"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV1993669.1"
                     /translation="MSTILIHADRALTPTTEINDASILIREDTIEAIGPRSGMTLPAG
                     AQEIRATGKTAVPGFIDVHIHGAGGHDVMEGTDAALRSITRKVSEFGSTSLVATTVTA
                     STDQTLRAVEGIAGYIAQQRETDEPRAEILGIHFEGPFISKERRGVHPAEWIQLPSLE
                     TLSRFLKAAAGNARILTIAPELLGAAPCIDAAREAGLIVSMGHTDATYEQARAAMARG
                     ARSATHVYNAMRPFSHRDPGVIGAVLTSPEINAELIADGVHVEEGAMKLLLLAKGAAH
                     VTLVSDGLSATGMPDGKYTLGGFEVTVSGGVCRNAEGILAGSTLTLDRALRNIVALGA
                     SLPEAVRMLTLNPATLLGIEYKKGSLRVGADADILLLDEGLHVAGVWARGVALN"
     gene            complement(8645..8980)
                     /locus_tag="VGR03_05015"
     CDS             complement(8645..8980)
                     /locus_tag="VGR03_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5818 domain-containing protein"
                     /protein_id="HEV1993670.1"
                     /translation="MKVRFAVAMAATLFAGALAMAADGSWTGYISDSACGVKGAKEGQ
                     GECTTKCVKEHGAKYVFVNDADKKVYAIDAQDKVAAHSGHHVTVKGTVDGETLKLSSI
                     DMAAPAKTM"
     gene            complement(9196..10038)
                     /locus_tag="VGR03_05020"
     CDS             complement(9196..10038)
                     /locus_tag="VGR03_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993671.1"
                     /translation="MSESQDSANTSTTGTPRWVGLAVAVLGGISLLGLGVGWSALNQA
                     KGIEQTTQASLKQSNDALAQRLAKEDEINQQLQSDVKVVTDKLNVTHADLVAARNQNK
                     HATVAVDQKVTNLATSVKAELANKANTDDVNKLNGDVTGVKTDLDATKNSIQMARSEM
                     GTLIARNHDEIDQLRRIGQRDYFEFTVQRKGGATKVGAIQIELKDTNTKKNRYTINVL
                     ADDNSFEKKDRSVNEPIFFYTGGTRAALELVVNKVTKTTATGYLSIPKSAGAVSASTA
                     TSGQ"
     gene            complement(10783..11649)
                     /locus_tag="VGR03_05025"
     CDS             complement(10783..11649)
                     /locus_tag="VGR03_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV1993672.1"
                     /translation="MKVLRTGVLLILLSVLLVVAGGAIGGQSGLKIALGIAVLMNGVS
                     YFFSDKIALASSHAQRVSREQVPRLYEVMERLAAKARLPMPKLYVIPEPAPNAFATGR
                     NPQHASVAVTQGLLELMNDDELEGVIAHELSHVRNYDILTSSIAATIAGAITYLASMG
                     RWAMLFGGFGRDRDDDREGGGLAALLMIFLAPFAALMLQLFLSRTREYSADETGARMV
                     GQPYGLISALQKLGAYNQRIPTTSVSPSTSALCIVKPMFGGGTLSSLFSTHPPLEKRI
                     QALREMTVTPQR"
     gene            complement(11749..14370)
                     /gene="clpB"
                     /locus_tag="VGR03_05030"
     CDS             complement(11749..14370)
                     /gene="clpB"
                     /locus_tag="VGR03_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEV1993673.1"
                     /translation="MRFEKMTVKAQEAVQSAQEVAARHENQQIEPVHLLASLVAQADG
                     VVSPLLARLGVRSEALSQEIDREIGRLPKVQGFAQQHMGRALNDVLERAFKEADSFKD
                     EYVSTEHLFLAIARLDRDPAGQLLKRQGASHEAILQALTGVRGTQRVTSQNPEATYAS
                     LEKYARDLTELARRQKLDPVIGRDEEIRRVMQILARRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVSGDVPDVLKTKRLVALDLAALVAGAKFRGEFEDRLKAVLKEITEAEGRIILF
                     IDELHTLVGAGAAEGAMDASNMLKPALARGELRAIGATTLNEYKKYIEKDPALERRFQ
                     PVLVSEPTVEDTIAILRGLKERYEVHHGVRIKDAAILAAATLSNRYISDRFLPDKAID
                     LIDESAASLRMQIDSLPVEIDEIERRIMQLEIERQALLKETDAHSKERRKQIEKELAA
                     LKEDSSGRKARWQAEKEAIGKIRKLKEQIEQLKAEEQKYERSGELAKVAEIRYGKIAA
                     AERDLQKAQERFTAVQKNAPMLKEEVDEEDIAKLVSKWTGIPTGRLLEGEAQKLVHME
                     ERLRQRVVGQDDALERVSNAVRRSRAGLSDIKRPIGSFIFLGPTGVGKTELARALAEF
                     LFDDEKLMIRIDMSEYMEKHSVSRLIGAPPGYVGYEEGGQLTEQVRRHPYSVVLFDEI
                     EKAHPDVFNALLQILEDGRLTDSKGRTVDFRNTVLVMTSNVGSAAIFESSNKEAERAR
                     KEAMEALRASFRPEFINRIDEIVIFNPLGKEQLATIVGLLLKSVEKLLEERQITLELT
                     PAAQELLLREGYDPAYGARPLRRTIQRLIQDPLAMQILQGTVLPGDHVRVDRDGQKDT
                     MRFERAPSKQPLAAVGI"
     gene            complement(14459..14935)
                     /locus_tag="VGR03_05035"
     CDS             complement(14459..14935)
                     /locus_tag="VGR03_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEV1993674.1"
                     /translation="MRTLTRFEPFRGATTLQDQVNRLFNDVLERTGEESSLTAWAPSV
                     DIYETEHELVVKADLPEVDPKDLDIRVENNILTIRGERKFEKNVSEENYLRVERSYGS
                     FARSFTLANTVNSEAIKADYQNGVLKLSIPKREEAKPKQIKVSVGVPAMAAAAAGK"
     gene            15179..16276
                     /gene="queA"
                     /locus_tag="VGR03_05040"
     CDS             15179..16276
                     /gene="queA"
                     /locus_tag="VGR03_05040"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV1993675.1"
                     /translation="MRLDELDYHLPREQIAQRPLDRREASRLLFLNRPSGFFEDRFFT
                     EFPTLLRDDELLVLNNARVIPARLFGRRAGVHSQPPSRATKGEHLTGKVEIFLSRELD
                     SETWEALVRPGRKMQVGERVLFGDGELEAEVLGRGQLGLRTLRFISHDQSNISQHLER
                     LGHVPLPPYIERADETSDRERYQTVFAKRPGAIAAPTAGLHFSTEILEKIRGRGVEIC
                     ELTLNVGLGTFQPIHSETLEGHVMHAESYEIPAETANRIQAARDAGRPILAIGTTVVR
                     ALEDAALRAAESGSANLVLSGKAEARLFIVPGFRFRVVEGLLTNFHLPRSTLLALVCA
                     FAGREHVLAAYNHAVNAGYRFYSYGDCLLVR"
     gene            complement(16471..16803)
                     /locus_tag="VGR03_05045"
     CDS             complement(16471..16803)
                     /locus_tag="VGR03_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993676.1"
                     /translation="MKTNSFVRSMALLAAIALAVPAFAKPVLQNFQLSQKTLIGKSAL
                     NAGEYRFLIDGNRVTVTKGRDTLAESEGRWEEREKKYEYTELVSTTGGQLLELRFAGK
                     KSVFVLNQ"
     gene            17294..18037
                     /locus_tag="VGR03_05050"
     CDS             17294..18037
                     /locus_tag="VGR03_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1993677.1"
                     /translation="MRILVLSDLHSNATALDAVLDAAKDRWDQSVCLGDVVGYGPDPN
                     EVTARLRELGTQTIRGNHDKAVTGLMATDDFNPVAKSAVDWTRAQLKPEHLAWLSALP
                     HGPLATDGVVLVHGAFQDEDEYVFTPAQALDGLLDSTAAVTFFGHTHHQGGFSYQDSQ
                     LEVLQIRPRPSESFAALRIELPRRYLLNPGSIGQPRDGDPRAAFAIADLDHQVIEFWR
                     VPYDISAVQERMRVAHLPEPLVQRLVVGR"
     gene            18118..19596
                     /locus_tag="VGR03_05055"
     CDS             18118..19596
                     /locus_tag="VGR03_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993678.1"
                     /translation="MEFAAELQASLQEFTASGVVEVRENGGCVAPFSGMSWEVRGNGE
                     KPLLHLWSEQFNLTRRVLAITDHSEQRLALAVERFGRSKPDRLEFIRREFERGARELS
                     REEFRDRLSHLLAEQFPDETIESLAASPDLEHSLSGNYARGILRRGSAYVAVLAVPGG
                     EATDASDNSLTFALLWLRHARQSSCRGALTGVRIILPKNACGIVARRLAALDPQLAVE
                     LYEHDPALNILEKIDPRRAGNLDTWLVPHRESEALLDQARPALDAIIALAPQAVTLHP
                     AAQSREVWLRFRGLPFARWQDGRVFFGINDVREELTPASRPALKHLLHDLEVHRHPLA
                     SDTRHPLYRGQPERWLESIVHEDVTRIDAMLDQRFVYAQVFANAGGEHGILDLLTVTR
                     SGRVAIIELKANEHIHLPLQAADYWLRVRRQLQSGEIARYGYFPGVALQQASPLVYLV
                     APALRFHPTTDDLLNYLSPELEIVRVGLAESWRRGLRVVMRQ"
     gene            19730..20104
                     /locus_tag="VGR03_05060"
     CDS             19730..20104
                     /locus_tag="VGR03_05060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV1993679.1"
                     /translation="MQPTNFSVTIRQAGPISLLDVSGRLTSFETGALRDSISRLLKQG
                     RKNIVLNLSGLQYLDSSGIGELARIYVSVVKASGQMKVVGLSPKVEEILKITQLYQVF
                     PEFPSEEAALLSFPESSRKETI"
     gene            complement(20279..20926)
                     /locus_tag="VGR03_05065"
     CDS             complement(20279..20926)
                     /locus_tag="VGR03_05065"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV1993680.1"
                     /translation="MGTNRHMSVDSRGRYTPEAAGEAMPGSEGPAGSRSHNLISPHRA
                     SLMQDPVLVLNATYEPINVTAVRRAMVLMLKGVAQAEEMHNTEVHSAAHAHKVPSVIR
                     LLAYRHIPQQSRALSRKNILLRDRNTCQFCVRVFPCSELTLDHVVPRSRGGRSTWENL
                     VACCYRCNNSKGDRTPEEAGLKLARRPRPFTLHTSRQLMRLIGHRDERWRKYLFY"
     gene            complement(20947..21792)
                     /locus_tag="VGR03_05070"
     CDS             complement(20947..21792)
                     /locus_tag="VGR03_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV1993681.1"
                     /translation="MADGNSSSGTAAARRGGVRGGLRPGWGWLAEASDAIVSVFFPAG
                     CRICDRLLTSASRVPICGECLSSFKRVPSIVCEVCGRPLPGLTREPEQPLLCPACGDK
                     TYAFDRARSFAVYEDAVVRAILLLKFEQIEPLGAWFAERLAEVVNAEGDRLAADVVVP
                     VPLHRERERERGYNQAALLSKPLARRLRLPHKAVLLMRTRARPDKQVLSLEERWESVR
                     GAFATRPGSQVDNLRVLLVDDVLTTGATLDACARALRTAGAKSVIGLTVARAVRNPLP
                     STGEW"
     gene            complement(21942..22520)
                     /locus_tag="VGR03_05075"
     CDS             complement(21942..22520)
                     /locus_tag="VGR03_05075"
                     /inference="COORDINATES: protein motif:HMM:NF044994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEV1993682.1"
                     /translation="MPLQENPLLGRNEAHKEGVKLDTPVKSAYTTEFLDGPEEHVVGR
                     DRCGREQQQSHANKGMHKSMIKGTDKVKAGMDKKRLEYYKKKLQVRREELTKTIARTQ
                     EEGRTADDDATVDLADKAANSYTKEFLFGMTNTDRKILNMIDAALKRIQADEYGVCAN
                     CQEELQQKRLEAVPWAKHCLACQEKMEQGLLQ"
     gene            complement(22697..23308)
                     /locus_tag="VGR03_05080"
     CDS             complement(22697..23308)
                     /locus_tag="VGR03_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV1993683.1"
                     /translation="MYCDRCGMQLTAGAQFCSKCGKAIVGGGAATPPAAGRGMGQAAG
                     PAGAYGVAAGADGRVQRHLRTVATLWTINGILRLAGVCWMMLFGSMVFPFLRGWGRGV
                     VWPFGGRWGWDIPFLGGLFSVGIFLGLFGVLHLVLAWGLFEREPWARMLGLALGFLAL
                     LRFPLGTALGIYTLWVLLPESSGAEYDRLARGSEKLNSAAVSS"
     gene            complement(24176..24943)
                     /locus_tag="VGR03_05085"
     CDS             complement(24176..24943)
                     /locus_tag="VGR03_05085"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEV1993684.1"
                     /translation="MNIEQRRAEPRDAVLIPNGNVRRSKGQIFYNPSRSPGRVVFSIA
                     HEISHTFFPRTLTGARFRDLTASDSREANELERLCDLAASELLMPIEEFRAEGCNFFS
                     LTRTEELAEKFGSSFEATVFRLATAHPEVAVAGLLRYRRRIDEERKLAGSFQVSLFSD
                     LKRASDEVPSPKYRRQSFFTSENCEDEHTIRWNKSFGPESCVYVAARSDTICSAFERL
                     PNKSGQAGLLEVVRAPFQRDAAHPEFPDLLFFLSASG"
     gene            complement(25160..25594)
                     /locus_tag="VGR03_05090"
     CDS             complement(25160..25594)
                     /locus_tag="VGR03_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1993685.1"
                     /translation="MDSKSIDLTRLGYRIRFLRKGKNWSLADLAKESGLSKAYLSDLE
                     NGSAGKPNIQYIYSVAVALGVTLSELLQESGARNEGGRPKRGESELPSGLRDLQLELN
                     LSDDDVEKLAQVSFRGNRPRDKEGWLYLIETLNMLGQRKPRN"
     gene            25778..25930
                     /locus_tag="VGR03_05095"
     CDS             25778..25930
                     /locus_tag="VGR03_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993686.1"
                     /translation="MRKKVEFDAHKVVKKPTEVGFTTRNGKEVDFVARKNTKVPVHVR
                     FKANVK"
     gene            26579..27373
                     /locus_tag="VGR03_05100"
     CDS             26579..27373
                     /locus_tag="VGR03_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993687.1"
                     /translation="MWRQYESGRAKLSALEPGLLEALPGSLTGLIGAIKERQTYKRYY
                     EAVADYSFMAVPIASLVAPQWGADLDFVDEIVSASSKDMNIEQQLLFAMSEGKITEPI
                     VTGNQVWFTSPRRDLFAHPVPTVREIGEGEFEIVIRAASRPNYIQVAAVENRLLLTNG
                     VHKVCALHKMGYTHCVCVFRRANDLTEAGIDQRQTTLFRDAVFKSMRPALVTDFLEPL
                     AAAPLKMRAMYQVLQIAFSVGTITVPALPKNHSTQKPRVGAAATNC"
     gene            complement(27449..29242)
                     /locus_tag="VGR03_05105"
     CDS             complement(27449..29242)
                     /locus_tag="VGR03_05105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEV1993688.1"
                     /translation="MQKYLQENLKLAEQAVLFFAAFVLTLGLAAPPVVAAEIHEVTVE
                     RNVAAKMRDGVTLRADIYRPKTEGRYPVLLVRTPYDKTGSMGFGLKAAARGYVVIAQD
                     VRGRFESEGEWYTFKYESQDGYDTVEWAAALPYSNGKVGMFGGSYVGATQFLAALAKP
                     PHLAGICPTVTASNYHDGWTYQGGAFEQWFNESWSTGLATNTMRRRAEKKADALGGTN
                     VLPLISYPVLEAPSAEGIAPYFKDWLAHPNFDEYWKQLSIEDHYAQIEVPVLSFAAWY
                     DIFLGGSLKNYVRLKTEGGTEAARKGQRLVVTVGGHAGQSSTGKVGAVEFGSKLPADG
                     DELTLRWYDWLLKGETNGVEKEKPVKIFVMGKNEWREEDDWPLARAKNTKYYLHSAGA
                     ANGIAGNGALSTVAPAEEKADQYVYDPSDAAPTIGGPLCCGALPTGIGPEDQRPAEAR
                     GDVLVYTTPAFAKDTEVTGPVSLDLYVSSSAVDTDFTGMLVDVWPNGFAQNLTSGILR
                     LRYRNSQEKPELANPGETYHITVDLWATSNVFLAGHKLRLEVSSSNFPRFDRNLNTGE
                     EQARATRMLKATNVVYHDKAHPSALIVPVIP"
     gene            29592..29765
                     /locus_tag="VGR03_05110"
     CDS             29592..29765
                     /locus_tag="VGR03_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993689.1"
                     /translation="MKKLFLLACLIVLVAPYSAFAGHKRHIRADEMAGIGVGAAALVG
                     AAGYLLLRRRHSA"
     gene            29910..30800
                     /locus_tag="VGR03_05115"
     CDS             29910..30800
                     /locus_tag="VGR03_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HEV1993690.1"
                     /translation="MLALALTLVAIAADQFAAPILHSTSPLWATTACLLLVWRRDKFT
                     SSADDPPLELSISMGRLAAFLAAHSLLILSARSLSGTFQAVSGVMTFGGTLVAVCKLG
                     VLAPTIILFSPAVWKKIATIYFPEAIAGLVVLLTFFPSRTLETIWPWYGQVLGRFVHT
                     LARIFVPRLAYLADSNPTLSGPELDVTIVPECSGINGLELYDYLFGVVAFFDWNRLRK
                     GRALTAYFAGLFAMLLGNAIRITSFVVLGNHGFAESVSRFHISAGWIFFSVVFLVYLS
                     MTYSWMLGKRDTAVKHQQMS"
     gene            31112..>31329
                     /locus_tag="VGR03_05120"
     CDS             31112..>31329
                     /locus_tag="VGR03_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993691.1"
                     /translation="MYSAPRSILAVALFFMPSFSGTVAFPQAVKFIRHAADPLPLANL
                     PVGRAPCTKDSGSAVSAPYGSTRATGDAG"
BASE COUNT         6320 a         9601 c         8646 g         6762 t
ORIGIN      
        1 cggcgcgtct atgcctcgca tggtacggaa gtgaaggtgg ccgacgcgga cagcggcgcc
       61 gtggtcggaa cgatttccgg attgaaacag tgccacggga tagtcctggt ggaagaactc
      121 ggcaaaggat tcatcagcga cggcggggcc gccaagacgg taattttcga tatggcgagc
      181 ttgaaggtga ccggcgaggt caagggcgag gaagatgccg actccataat ctacgatccg
      241 gcgtcgaaac gcgtttttgt gttcaacggc gatgccaaga gcgcgacggt gatcgatccg
      301 gcgaacggga cggtggtgaa gagcattccc ttgggaggtg gaccggagtt cgcggtggcc
      361 gacggcaagg gaatggtgta cgacaacatt gaagacaaga atgaagtggt ggcgatcgat
      421 tccggcacgc tggcgatcaa gtcgcggtgg ccggtggcgc ccgcggggca accgacttcc
      481 ctagcgatgg accgcgagca ccggcggctt ttcgcggcgg gccgcaaccc gcagaagttg
      541 gtgattctgg acgcggacaa tggcaaggtg atccagtcgt ttcccattag cgccggtgca
      601 gacgcgaatg tttttgagcc ggagacgggt ctgatctttg tctccactcg cgaaggcatg
      661 atccacatct tccacgaaga cacgcccgac aagtatagtg aggtggagac ggtgaagacc
      721 gagttcgggg ccaagacgat ggcgctcgat tcaaaaacgc acaacctcta cttgacgacg
      781 gcggattttg gaccaccccc cgctcccaca gcggagcggc cgcacccgaa tccggcaccc
      841 attccgggaa cattccacct gctgatttac ggaaagtaag cgcagcggtc gcgttgacgt
      901 aacggctctt tgaatgctat aaaattaagt ttcgagacgg cagcgttaag cccaccgtgc
      961 gctcctacgg ggttggagcg cggctgccaa cctagtggcc ggttggcggc cacacttgac
     1021 ggaatttagg cgatccaatt tcgatgcaaa cgattgttca ccagctaggc gaactcttcc
     1081 ttcaggctgc gcccacagtc ctgattatct tcctttttta catcatctta cgggcgattt
     1141 tcttcaagcc gctgctggcc gtgatggccg agcgcgattc ccgcaccgtg ggtgcgcaaa
     1201 aggcatcgga agatgcccag gccgctgctg cccagaaggt caagcagtat caggaagctc
     1261 ttaaacaggc gcgcggtcag ctgtatgccg agcaggaggc cgctcgcaag aaacttctcg
     1321 aggagcgcgc cgcgcagatc aaagaggcgc gcacgcgcgc tgcgggcgaa gtcagcgccg
     1381 cgaaagagcg cgtggcggcg gaacttgccg ctgctcgccg cgacgtcgaa accaccgtcg
     1441 cgcaactttc cgccgaaatc gcgcgccgca tcctgcaaac tccgccgcgt cccggcgctc
     1501 ccgcgagcga ggctcgatga cgcgaaatcg tccccttttc ttggctgtga gtattctatt
     1561 tatgttgctc ttcgcggtcg ttggcgcgaa cgccgctgag gaaggtggca acgcggcaac
     1621 cgagcgcgcc acggaaatct tcaagtggat caacttcgcc atcgtcgccg gtttgattct
     1681 ttggctgtgt ttgaagaagg ctccgatttt cttcagcgga cgcgcttccg cgatcagctc
     1741 cgcgatcaca atcgctaccg ctgcgaaggc cgccgccgac gcgcaggttc gcgaagcgga
     1801 aaccaggctc gcgaatctcc agacggaagt cgcggaactc cgcgccactg ccgaacgtga
     1861 agccaccgcg gaggcagcgc gcctgcgagc cgccacgcag accgatgcgc agaaaatcgc
     1921 cgccgccgca agggccgaaa tcgaagccgc gggacgcgcc gcgcgcctgg agctgaaagc
     1981 gctggccgca aatcttgcgg tcgatggcgc ggaatcactc ctcgtcaagc agctcacccc
     2041 gcaggcgcag gaatcgctca tcagcaactt cgtgaaaagt ttggaaggga ggccgaattg
     2101 aaatccgcga gcctccagta cgcgaatgcg atggcggaca tcgcgctcgc gcagggcgcc
     2161 gccgagccgg ccgccaagca gctccacgaa tttggcgcgg cgtacgcgca atccgcggag
     2221 ctgcgcacgt ttctggcaag cccggcggtg agcgttgaag ccaagcacgc cgtcatcgag
     2281 aaaatcgtgg cgcggctggg tgcgagcaag atcatccgca attttctctt cgtgatcgcg
     2341 gaccaccggc gcacacagtt gattcccgag gttatcgccg cgtttcatca agtcatccgg
     2401 cagcgacagg gagttgcgga agcggaagtt tcttcggccg tcgaattgag cgcggcgcaa
     2461 aagaaagaga tggcggcgac gctggcgcgc ttgacgggca agaagattga aacaaagtac
     2521 gcgcttgatc cggccctgtt gggcggcgcg gtggtgcgga tcggggacac gatttacgat
     2581 ggctcgttgc gcggccggtt gaatgagatg cgcgcgcgcc tcgcggccga gtaagctgcg
     2641 attttgcaag tggggggcta aagcccggca cctacataag agattaggaa ttcagcgacg
     2701 gagcaggcat ggcgaatatt aaggcagacg aaatcagcaa gattctgcgc gagcagatcg
     2761 agaattacga gcagaccgtt tcggtcgacg aagtcggcgc ggtcatcagc gtcggcgacg
     2821 gcattgcgcg cgtccatggc ctcgagaaag tcatggccgg cgagatgctc gctttcccgc
     2881 ataacgtttt cggcatcgcg ctcaacctcg aagaggaaga agtcggcgtg gtgcttctcg
     2941 gcgaatcgca ggagttgaag gaaggcgacg tcgtcaagcg cacgaacacg attatgtccg
     3001 tgccggtcgg cgaagcgctt gttgggcgcg tggtcaatcc gctcggccag ccggtggacg
     3061 gcaagggccc catcgccacg aagcagcgca acccgctcga gaagatcgcg ccgggcgtgc
     3121 tggatcgcca gccggttcgc gagccgttgc agaccggcat caaagcaatc gactccatga
     3181 ttccgatcgg ccgcggacag cgcgagctga tcatcggcga ccgccagacc ggcaagactg
     3241 cagtcattct cgacaccatc atcaatcaaa agggcggcga catcatttgt atttactgcg
     3301 gcatcgggca gaaacgctcg acgatcgcgc aggtcgtgaa gacgctcacc gacgcgggtg
     3361 caatggacta caccatcgtc gttacctcct ccgccgcgga accggcttcg ctccagtaca
     3421 tcgcgccata cgccgcgtgt gcgatgggcg aatttttccg cgataccaag cggcatgccg
     3481 tcacgtttta cgatgatctt tccaaacacg cgcaggccta tcgcgaaatt tcgttgctgc
     3541 tccgccgtcc tccgggacgc gaagcgttcc ccggcgacgt gttctatctg cactcccgct
     3601 tgctggaacg tgctgcaaag ttgaatgaca agctcggcgg cggctcgctc acttcactgc
     3661 cggtcatcga gacgcaggct ggcgacgtct ccgcgtacat tccgacgaac gtgatttcga
     3721 tcaccgacgg ccagatttat ctggaggccg accttttcaa cgccggcatt cgcccggcca
     3781 tcaacgtcgg catttcggtc agccgcgtgg gcggcaacgc gcagatcaag gccatgcgcc
     3841 aggtggccgg ctctctgcgg ctggacatgg cacagtaccg cgaactcgcg gccttcgcgc
     3901 agtttggctc cgatcagctc gacaaggtga cccagggcca gctcgcccgc ggccagcggc
     3961 tcacggaaat cctgaagcag gatcagtatg tgccgctgcc ggtggaaaaa caggtactca
     4021 gtctctacat tgcgacgagt ggcttgctgg acgctgtgcc ggtcgcggaa gttggccact
     4081 ttgagcgcga gttcctgcag ttcgtcgaga ccaactaccc ttcgatcctg aagaacattg
     4141 cagcgaagaa agctctcgac gacgccatca agagcgaagt caagaccgcc gttgacgctt
     4201 tcaaggaacg tttcactgct gccgttgccg cggcagcgaa atgaggatcg gcgcttaacg
     4261 aataacgatg gccacacttc ttgattttcg gcgccgcatc cgctcggtga agaacaccca
     4321 gcagatcacc cgcgcgatga aattcgtcgc cgcagcccgg ctgcgccgcg cgcaggaagc
     4381 cgccattgcg gcgcgcccgt acgccaaaga gctcgcgcgc atgctgcgtt cgatcatgtc
     4441 gcgcatcgat gagccccagc acccgctgct ggcgaagcgc tccgaggagc ggattcttgc
     4501 actcgttctg acgggagagc gcggccttgc tggcgctttc aacgccaata ttctgcgcaa
     4561 ggcccaggat ttctttcgcg tcaacaaggg caagaaaatc tcgaccatcc ccattggcaa
     4621 gaaaggccgc gactcgctga aaaaggcggg cttgggcctc atcgcggaat atgtgaacgt
     4681 cctcgcgcgc gtcgattttg cgaccgcgcg ggagatcgcc ctgctcgtca cggatcttta
     4741 tgcaaaggaa gagatcgatt cggtgtacat cgtcttcagc gaattcaaga cggtgatgac
     4801 accgaatctt gtagtcacaa agattctgcc ggtcgaagcc gttcatgcgg acgaagaatc
     4861 cgccgccgaa aaagaaggtt cgcaggtgga ttacatctac gaacagcctg tagatcaact
     4921 gctcggcaaa ttgcttccgc gctacatcga aacgcagatt ctccgcgcga tgctggaatc
     4981 ttccgcggcg gagcacgccg cgcgcatgac cgctatggaa tcggcgacca agaatgccgg
     5041 cgacgttatt gatgccctga cgctgcacat gaacaaagtt cgccaggcgg ccatcaccaa
     5101 ggaaatcatc gaaattgtca gcggcgcatc ttacggcgct taggagaaag cagcatggca
     5161 gagaaatacg cgggcgtggg tcacgtcgta caggtcattg gacctgtcat ggacattcaa
     5221 ttcgaggcgg ggcacctccc ggcgattttt aacgccgtgc gcattaccag cgaaggctac
     5281 tcgataccca caccgctgga cgtgacggcg gaagtccagc agcacctggg cgaaggccgc
     5341 gttcgcgcca tcgccatgga gcctgtcgag ggcatggttc gcggtatgaa ggcctacgat
     5401 ctcggaaaag ggatcgaagt tcccgtgggc cggcccaccc ttggccgcgt gatgaatgtc
     5461 cttgggaaac ccgtcgatca gttgggaccc attgtgagcg atacgtacta tccgattcac
     5521 cgcccggcgc cgtcgctcga agatcaatcc acgatgcttg aaatgtttga aactggaatt
     5581 aaggtcgtcg atctgatcga accctatttg aagggcggca agattggtct cttcggtggt
     5641 gcgggcgtcg gcaagaccgt tctcatcatg gagctgatcc acaacgtcgc gatgaagcac
     5701 ggcggcgtct ccgtgttcgc gggcgttggc gagcgcaccc gcgaaggcaa cgacctgtgg
     5761 ctggaaatgt ccgagggtgg cgttattgtt cccggaaatt cggaaaaatc gcgcgccgcg
     5821 ctgatctacg gccagatgac cgagccgccc ggctcgcgtc tccgcgtggg cctcaccggt
     5881 ctcactgttg ccgaatattt ccgcgaccag gagcatctcg acgtgctcct cttcatcgac
     5941 aatattttcc ggttcgtgca ggcgggttcg gaagtttccg cgcttcttgg gcgcatgcct
     6001 tccgccgtcg gctaccagcc gaatctcaac accgaaatcg gtgagttgca ggaacgcatc
     6061 acttcgacga agagcggatc gattacttcg gtgcaggcca tttacgttcc cgccgacgac
     6121 tacacggacc ccgctcccgc cgctacgttt gcgcacctgg acgcgacgac caacctcagc
     6181 cgtcagattg tcgagcgcgg catctacccg gcggtcgatc cgctggcgag ttcctcgcgc
     6241 attctcgatc cgcgcatcgt tggcgccgag cactatgccg tggcgcgcgg cgtgaaatcc
     6301 attctgcagc gctacaagga cctccaggac atcatcgcca ttctgggtat cgaagaactt
     6361 tcggacgaag acaagcgcac cgtggcgcgc gcccgcaaga tcgaaaaatt cctctcgcaa
     6421 ccgatgttcg tcgccgcgca gttcaccgga ctcgaaggga agtacgtgaa gatcgaagac
     6481 acggtccgca gcttcaagga aatcgtggaa ggcaagtacg acgacgttcc ggaacaggcg
     6541 ttttacatgg tcggcacgat cgacgaagcc ctcgcgaagg ccgaaaaaat gaaggcgagc
     6601 gcctaattac gaaaatgctt cctgatgcca ttgaacttgt aatcgtcacg ccggagcgcc
     6661 agttgctccg cgagaaagcc gtggaagtgc agcttccggg ggagggcggc tatcttggcg
     6721 tactcccggg gcacgcgccg ctgatgaccg agctggggat cggcgaactc agctatcacg
     6781 atctcggtgg caaggagtcc gcgcatctcg ctattgttcg cggatttgcc gaggttcttt
     6841 cagatcgcgt aacggtcctt gcagaaaccg ccgagcgcgc cgaagagatc gacctgcagc
     6901 gcgccaaaga tgcgctggct cgcgccgaga agcgcctggc ctcgaacgat cccaacatcg
     6961 attgggatcg cgcaaacatc gcgctccagc gcgctttgat ccgcatccag gttgcaacca
     7021 agcggcgcag cgccctcgtt gcaggctcct gacgcatcat ctaccgtttc tcgaatgagg
     7081 attcgtgcga cttcgcggtg ccgtccgcat ctaatagagt ggagcagacg atggaaacca
     7141 tcaccatgta cagcaccacc tggtgccccg attgccgccg cgcaaaatcc tttttgaagg
     7201 aacgcggggt caattttcgc gaagtgaata tcgaagaaga tccgtcgggg gaagcgttcg
     7261 tcatcaaggc caacaacggc aagcgcacag ttcccaccat tgaagtcggg ggccgctatt
     7321 ttgcctgcag tcccttcgat gcggagcagc ttgccgagga actcaacatt ccgctgaatc
     7381 cctaattcag cgcaacacct cgggcccaaa ctcccgctac atgcaatcct tcatcgagca
     7441 gcaaaatatc cgcatccgct cccacacgca gtgaaccttt cttatattcg atcccgagca
     7501 atgtggcggg attcagcgtt agcattcgca cggcctccgg caaagatgcg cccagcgcta
     7561 caatatttcg cagcgcgcgg tccaacgtca gcgtactgcc cgcgagaatg ccttcggcat
     7621 tccggcagac gccgccgctg accgtcactt cgaagccgcc gagtgtgtac tttccgtccg
     7681 gcatgcccgt cgcggagagg ccgtcgctta ccagggttac gtgagcggct cccttggcga
     7741 ggagaagcaa tttcatggcg ccttcttcga cgtgcacgcc gtcggcgatc aactccgcat
     7801 tgatctccgg cgaagtgagc acggcgccga tgacgcccgg atcgcggtgg gaaaacggac
     7861 gcatggcatt gtagacgtgt gtagcgctgc gcgcccctcg agccatggcc gcacgagctt
     7921 gctcgtaggt tgcgtcagta tgtcccatcg aaacgatcag gccggcttcc cgcgcggcgt
     7981 cgatgcacgg agcggccccg agcagttccg gcgcaatcgt cagaatcctg gcattccccg
     8041 cggcggcctt gaggaagcgg ctgagcgtct ccagcgacgg cagctgaatc cattcagctg
     8101 gatgaactcc gcggcgctcc ttgctgatga agggcccctc gaaatggatt cccaggattt
     8161 ccgcgcgggg ttcatccgtt tcgcgctgtt gagcgatgta ccctgcgatt ccctcgaccg
     8221 cacggagagt ctggtccgtg ctcgcggtta ccgtggtggc gacgagagac gtgctgccaa
     8281 attccgagac cttgcgagtg atggagcgca acgcggcgtc agttccttcc atgacgtcgt
     8341 ggccgccggc accgtggatg tgcacgtcga taaagccggg aacggctgtc ttgccggttg
     8401 cgcgaatttc ctgggcccct gcgggcaggg tcatccccga gcgcggcccg atagcctcaa
     8461 tggtatcttc gcgaatcagg atgcttgcgt cgttgatttc tgtcgtcggg gttagcgcgc
     8521 ggtcggcgtg aatgagaatg gtcgacatgg cagcactaca tcgtaacgga aatgcttcgc
     8581 ggcacaaaac aaaacgggcg gagccgaagc gccgcccgtc tcaaaaaagc tccgcaaaca
     8641 gctcttacat ggtctttgca ggtgccgcca tgtcgatgct gctaagcttg agcgtttcgc
     8701 cgtccacggt gccctttacc gtcacgtggt ggccggaatg cgccgccact ttgtcttgcg
     8761 catcgatcgc gtacaccttc ttgtcggcgt cgttcacgaa cacatacttc gcgccgtgct
     8821 ccttgacgca cttggtggtg cactcgccct gaccttcctt ggcgcccttc actccacaag
     8881 cgctgtcgga aatgtagcca gtccatgagc catcggccgc catcgccagt gctcccgcga
     8941 aaagcgttgc tgccattgcc actgcaaatc gtactttcat gatttccccc tccagaaatc
     9001 cactgcggtt tttaatcccc tgggccgcca ttagcttggc acagcgacgg tcaggtgtcc
     9061 agactctatc taagaccgag tatggagtat tcttattccc ggatgagcat caataaaaaa
     9121 agggaggatt ctctggaatc ctccctatct tggtgtgctc tcaaggattt gaagactaac
     9181 ttctgcggaa ctcgcttact gcccggacgt cgctgtgctc gccgagaccg ctcccgcgga
     9241 cttcggaatg ctcaggtagc ccgtggccgt tgtcttggtg actttgttga ccaccagttc
     9301 gagcgctgcg cgggtaccgc cggtgtagaa gaagatgggt tcgttcaccg aacgatcctt
     9361 cttctcgaag ctgttgtcat ccgccaggac gttgatggtg tagcggttct tcttggtgtt
     9421 ggtgtccttc agttcgatct gaatggcgcc gaccttcgtt gcaccgccct tgcgctgcac
     9481 ggtgaactcg aagtaatcgc gctggccgat gcggcggagc tgatcgattt catcgtggtt
     9541 ccgcgcgatg agtgtgccca tttcgctgcg cgccatctga atgctgttct tggtggcgtc
     9601 caggtcggtc tttacgccgg tgacgtcgcc attcagcttg ttcacgtcgt cggtattggc
     9661 cttgttggcg agttcagcct ttacggaagt cgcgaggttg gtcactttct ggtcgaccgc
     9721 gacggtcgcg tgcttgttct gattgcgtgc cgcgactaga tccgcgtgcg ttacgtttag
     9781 cttgtccgta accaccttca catcgctctg cagttgctgg ttgatttcgt cttccttcgc
     9841 cagccgctgg gctagtgcat cattggattg cttcagagaa gcttgtgtcg tctgttcgat
     9901 gcccttggct tgattaagtg cgctccagcc tacgccaagg ccgagcagcg agataccgcc
     9961 caataccgct actgccaatc cgacccaacg gggtgtccca gtcgtgctcg tgttggctga
    10021 atcttgagat tcgctcatat ccttcgtttc tccttttggc tcttcgccct gtgccatgga
    10081 agggagcatc cccctcgcca aagggctctt ccgcgcttcc ttacgaataa gtcatgtaga
    10141 atcaattgat tagagaaaat tatagggaag acattgggat agttccatgg gtaccagtac
    10201 cagtaaagat tacgcagtcc cctcgaaaat cgtataattt gctgtcagta cttttaggaa
    10261 catcctttga cttgatttgc gagaatcaac gaaatggaaa aatcgagagg aggtaagacc
    10321 agaggcgtta agcttctatt tttcaattag ttagcgtgag gcaaatcagg cggcgcggga
    10381 actcgcatcg gccccggcag tgcgctcgga gctgaaatac cggtccagcg tgttgattgc
    10441 agcgtctaga accaggtggc agtcggagca ggtatccaga tggcgtttga tctcgccttc
    10501 cacggacgtg gagagccgcc cctctaagta ccaacaaatc aaatggatag tgtccttaca
    10561 gctcatatcc cccgtccccc aggttcccaa agacgacgat gcggcccacc gttggctgtg
    10621 catttcccgc gccaaacact gccttgcaac cggaaatatt atctgctgat aaatcaaagg
    10681 tttacccagc gttacagaat ttcaatgcag tgcgcgtgct gcaaatcttg aaaagattac
    10741 acttggggtg gttcaaatat gcagaatctc gtcaccagat ccctatcgct gcggggttac
    10801 cgtcatctcc cgcagcgcct ggatgcgctt ctccagcggg ggatgcgtac tgaacagaga
    10861 tgacaaagtg ccgccgccga acatcggctt tacgatgcac aatgctgagg tcgacgggga
    10921 aaccgatgtt gtgggaattc gctggttgta agcacccagc ttttgcagcg cgctgatcag
    10981 gccataaggc tggcccacca ttcgcgcacc tgtttcatcg gcggagtact ctcgcgtccg
    11041 cgaaagaaag agttgtagca tcaaggccgc aaatggcgcc aggaaaatca tcagcagtgc
    11101 cgccaacccg ccgccctcgc ggtcatcgtc gcggtctctt ccgaagccgc caaagagcat
    11161 ggcccagcga cccatggacg cgagataggt aatggctccc gcaatcgtag cggcgatcga
    11221 actcgtcagg atgtcgtagt ttcggacgtg agaaagctcg tgggcgatta cgccttcgag
    11281 ttcgtcatcg ttcatcaatt caagcagtcc ttgggttacc gccacagacg catgctgcgg
    11341 gttgcggccg gtggcgaagg cgttcggcgc cggttctgga attacataga gcttcggcat
    11401 cgggaggcgc gccttggcag cgagacgttc catcacttcg taaagcctgg gcacctgctc
    11461 gcgcgaaact ctctgtgcat ggctggaagc cagcgcgatc ttgtccgaga aaaaatacga
    11521 gacgccgttc atcaagacgg cgatgcccag cgcgatcttc aggccactct gcccgccaat
    11581 cgctcctccc gctacgacca gcagcacact cagcaggatc agcagcacgc cggttctcaa
    11641 gactttcatc ggttcctcat tcatctagtg tacaaaaatg cgtcgcgcac gtcagcgggc
    11701 aagcgagagt aagacgtttc atccacagcg gtgtctcgcc accccaattt agattcctac
    11761 ggcagccaga ggttgtttcg atggcgcgcg ttcaaaccgc atcgtgtctt tctgcccgtc
    11821 gcggtctacg cgcacgtgat cgccgggcaa caccgtgccc tgcaggatct gcattgccag
    11881 cggatcctga atcagccgct gaatggttct gcgcaacggc cgcgctccat acgccggatc
    11941 gtagccttcg cgcagcaaca actcctgcgc tgccggcgtc agttccagtg taatctgccg
    12001 ctcctcgagc agcttctcca cgctctttag cagcaagccg acgattgtcg ccaattgctc
    12061 cttcccgagc gggttgaaga tcacgatctc atcaatccgg ttgatgaatt ccggtcggaa
    12121 cgacgcacgc aatgcctcca tcgcttcctt tcgcgccctc tccgcctctt tattcgacga
    12181 ttcaaagatc gccgccgacc caacattgct ggtcatcacc agcacggtgt tccggaagtc
    12241 cacggtgcgt cctttgctat cggtgaggcg cccgtcctcg agaatctgca acagcgcgtt
    12301 gaacacatcc ggatgcgcct tctcaatttc gtcaaacaag accaccgaat acggatgacg
    12361 cctcacctgc tcggtgagct gtccgccctc ctcgtagcca acgtaaccgg gaggcgcgcc
    12421 gatgagccgc gacacgctgt gcttctccat gtactcgctc atatcgatgc ggatcatcaa
    12481 cttttcatcg tcgaagagaa actccgcgag cgctcgcgcc agttccgttt tccccacgcc
    12541 tgtgggtccg aggaaaatga acgaaccgat cggccgcttg atgtcggaga gacctgcgcg
    12601 gctgcgccgc acggcgttcg acacgcgctc cagcgcgtca tcctggccga caacccgttg
    12661 ccgcagccgc tcttccatat gcacgagctt ctgggcttcg ccttccagca atcggcccgt
    12721 cggaatgccc gtccacttgc tcaccagttt cgcgatgtct tcctcatcga cttcttcctt
    12781 caacattggc gcgtttttct gcaccgcagt aaatcgttcc tgcgccttct ggaggtcgcg
    12841 ctccgccgcc gcaatcttgc cgtagcggat ttccgccacc ttggccagtt cgccggagcg
    12901 ttcgtatttc tgctcttccg ccttcagttg ttcgatctgt tccttcagct tgcgaatttt
    12961 tccgatggct tccttttctg cctgccaccg tgcctttcgc ccgctcgaat cttctttcag
    13021 cgccgccagt tccttctcga tctgcttgcg ccgctccttc gagtgcgcat ccgtttcctt
    13081 caacagcgcc tggcgttcaa tttcgagctg catgatgcgc cgttcgatct cgtcaatctc
    13141 gaccggcaga gaatcaatct gcatccgcag gctggccgcg gactcatcga tcaagtcaat
    13201 ggccttgtcc ggcagaaacc ggtccgagat atagcggttg gaaagcgtgg ccgccgcgag
    13261 gattgcggca tccttgatgc gcacaccgtg gtgcacttca tagcgttcct tcaatccgcg
    13321 caggatggcg atggtgtctt ccaccgtcgg ctcgctcacc agtaccggct ggaacctgcg
    13381 ctcgagcgcc ggatctttct cgatgtattt cttatattca ttgagcgtcg ttgcgccgat
    13441 cgcgcgcaac tccccgcgcg ccagcgccgg cttgagcatg ttggacgcgt ccatcgcgcc
    13501 ttccgcggcg cctgctccca ccagcgtgtg tagctcgtcg atgaacagaa tgattcgccc
    13561 ctcggcttcg gtgatctctt tcaggacggc tttcaagcgg tcttcaaatt ctccgcggaa
    13621 tttcgcgccg gccaccagcg ctgccaggtc cagagcaacg aggcgcttgg tcttcagcac
    13681 gtccggcacg tcgccggaga cgatccgttg cgccaagcct tccacaatcg cggtcttccc
    13741 gacgccgggc tcgccgatca gcaccgggtt gtttttcgtg cgccgcgcta gaatctgcat
    13801 cacgcggcga atctcttcgt cgcgcccgat caccggatcg agcttttgac gccgcgccaa
    13861 ttccgtgagg tcgcgcgcat atttttccag cgatgcgtag gtagcctcgg gattctgcga
    13921 agtcacgcgt tgcgttccac gcactccggt caaggcctga agaatggctt cgtgtgaggc
    13981 accctggcgt tttaacaatt gcccggcggg gtcgcggtcc agcctggcaa tcgccagaaa
    14041 caggtgctcc gtcgaaacat actcgtcttt gaagctgtcc gcttccttga acgcgcgctc
    14101 cagcacgtcg ttcagcgccc gccccatgtg ctgctgagca aaaccctgca ccttcggcaa
    14161 ccggccaatc tcccggtcaa tttcttgcga cagcgcttcg ctgcggactc ccagtcgcgc
    14221 cagcagcggt gacaccacgc catcggcttg ggcaaccaat gacgccagca ggtgcacggg
    14281 ctcaatctgc tgattctcgt ggcgcgcggc aacttcctgg gcactctgca cggcctcttg
    14341 cgccttgacc gtcatttttt caaatcgcaa aatacctgcc tcatttcatg ctgccgctgc
    14401 agaaaagggg ctggcaccct ggccagcccc ttattctaac tgcgatttcc aattgaaatt
    14461 atttgccggc ggcggccgca gccatcgccg gaaccccgac gctgaccttg atctgcttcg
    14521 gcttcgcttc ctcgcgcttc gggatgctca gcttcagcac gccgttctga tagtcggctt
    14581 tgatcgcttc cgaattcacc gtgttcgcca gcgtgaagct ccgagcgaag gaaccatacg
    14641 agcgctccac gcgcaggtaa ttctcctcac tcacattctt ctcgaacttg cgctcaccgc
    14701 gaatcgtcag gatgttgttc tccacgcgga tgtccaaatc ctttggatca acttccggga
    14761 gatcggcttt gaccaccaac tcatgctcgg tctcataaat gtccaccgac ggcgcccagg
    14821 cagtgaggct ggattcctcg cccgttcgct cgaggacatc attgaacagc cgattgactt
    14881 gatcctgcag ggtagtggct ccgcggaatg gttcaaatcg agtaagtgtt ctcatgtttt
    14941 atgcacctcc atgcgtgact gcatcaatat aataaaatat gagtgccaat atgtcaagta
    15001 caaatttgca gttctctaga acatgtttaa gctattgtat ttaaacaggt tatagctaat
    15061 ctggacttgc aggaaccatc ttcactttta gctcaatctt ggcgaaaatc cttgtttcga
    15121 agtgtaattc gtttgctttg tttgccttag ccgctttgct gttagactgc cgcttcccat
    15181 gcgattggac gaactcgact accatctccc tcgagagcaa attgcgcagc gtcccctcga
    15241 ccggcgcgaa gcctcgcggc ttctgttcct gaatcgtccc tcaggatttt tcgaagaccg
    15301 attcttcacg gagttcccaa ctctcctacg cgatgacgaa ctcctcgtcc tcaacaatgc
    15361 gcgcgtcatt ccggcccgct tgttcgggag gcgtgccggc gtccattcgc agcctccctc
    15421 gcgggccaca aaaggggaac atctgacggg aaaagtcgaa atcttcctca gtcgcgaact
    15481 cgattccgag acttgggagg cgctggttcg gcctggccgg aagatgcagg taggggagcg
    15541 ggttctcttt ggcgatgggg aacttgaagc tgaggttctc ggacgcggtc agcttggctt
    15601 gcgcacactg cgcttcattt ctcatgacca gagcaacatc agccagcatc tcgagcgcct
    15661 cgggcatgtt ccgcttccgc cgtacatcga gcgcgccgat gaaacttctg accgcgagcg
    15721 ctaccaaacc gttttcgcga agcggcccgg cgcgatcgcc gcacccaccg cgggtctgca
    15781 cttctccacg gaaattctgg aaaaaattcg cgggcgggga gtggaaatct gcgaacttac
    15841 gctgaacgtc ggtctcggca cgtttcagcc gatccatagc gaaacgctcg aaggccacgt
    15901 gatgcatgcc gaatcttacg aaatcccggc agaaaccgcg aatcgtatcc aggcggcgcg
    15961 cgacgccgga cgccccatcc tggccatcgg cacaaccgta gttcgcgcgc tggaagacgc
    16021 cgctctgcgc gcggccgaat caggttctgc gaatctcgtg ctttccggca aggccgaggc
    16081 gcgcttgttt atcgtgcccg gattccggtt tcgcgtcgtg gaagggctgc tcacaaactt
    16141 tcatctgccg cgttccacgc tgctcgcgct ggtctgcgct ttcgccggcc gtgagcacgt
    16201 cctggcggct tacaaccacg cggtcaacgc gggctaccgc ttctacagtt atggagattg
    16261 cttgctggtt cgttagtgtg tgggaagagg cgtttggcga ttcgagataa ccttgcgcat
    16321 gcccagcgca agaaattgca gcaacccgct cgccgctgaa accggatctt gccacttcag
    16381 aagttgcgcc gccagtacga caagcagaga ggccgcaccg gagtgcagcc ccccctcgtc
    16441 ccttggaaag gagtcacgga ctctccccgc ctactgattc aagacgaaaa cgctcttctt
    16501 gccggcgaac cgcagttcta acaactggcc gccggtggtc gacacgagtt ccgtgtactc
    16561 atacttcttt tctcgctctt cccagcgccc ttcggattcg gccagggtat cccggccttt
    16621 ggtgaccgtc actctgttgc cgtcgatgag gaagcggtac tcgcccgcat ttagcgcgct
    16681 tttgccgatc agggtttttt gagaaagctg gaagttttgc aaaacgggct tcgcgaatgc
    16741 cggtactgcc agtgcgatgg cggccagcaa agccatgctg cggacaaatg aattcgtttt
    16801 catgcgtgtc aatttccttt caaacaggat cgagtcagcc gcacagcagg ctgccattgg
    16861 ctagagagag ttgacacgca ggtggcggac gagtactctc gcccgtgaag acacctactc
    16921 ttcctggatt aacggccgga aagactgcgt gtcatgaccc cgcggctgcc accgcggggt
    16981 ttttctgaat ctcgccaaga acagccccgt aaccagctgc acccgtatag acgaaccact
    17041 gggggcgaat gtttcgtgag ctcgccagaa aattttgtcg ccagtcgaaa caaccagcgc
    17101 cactgtcgcc ctcgtttctc tctgctcgtg aactcaccat ctctccggtc tcaactattc
    17161 ataaagaaat acgcttttgt tccaaacctt ttaccttttc ttgcgtcgct ctggttcgca
    17221 atccgttgtg catcctgccg tgcatcggcg cgcttgttca tttatactcg gggtcaattt
    17281 tcggatacta agaatgcgga ttctcgttct cagcgatttg cactcgaacg ccactgcact
    17341 cgacgccgtc ctcgacgccg cgaaggaccg ctgggatcag tccgtctgcc tgggcgacgt
    17401 cgtcggctac ggccccgatc ccaatgaagt cacggcgcgc ctgcgcgagc tcggcacgca
    17461 gaccatccgc ggaaatcacg acaaagcggt caccggattg atggccacgg acgactttaa
    17521 ccccgtagcc aagtccgccg tggattggac gcgtgcgcag ctcaagcccg agcatctcgc
    17581 gtggctatcg gctttgcctc atggacctct tgcgacggat ggcgtcgtgc tcgtccacgg
    17641 tgcgtttcag gatgaagacg aatacgtctt cactcccgcg caggccctcg atggtctgct
    17701 ggactccacc gccgccgtca ctttcttcgg gcacacgcat caccagggcg gtttttccta
    17761 ccaggactcg caactcgagg tcctccagat tcgccctcgc cccagcgaat cctttgccgc
    17821 gctgcgcatc gagctcccgc gtcgctatct cctgaatcct ggttcgatcg gacaaccgcg
    17881 cgacggcgat ccgcgcgccg cttttgctat cgccgacctc gatcaccagg tcatcgagtt
    17941 ttggcgcgtg ccctacgata tttccgccgt ccaggaacgc atgcgtgtcg cccaccttcc
    18001 ggagcctctg gtccagcggc tcgtcgtggg ccgttgaggt cgtaaaggtt actgaccttt
    18061 tatcggaatc ttgaaaagat tagttccgca tctacgtatc tcatctataa tctggccgtg
    18121 gaattcgctg ccgaactgca ggccagcctc caggagttta ccgcctccgg ggttgttgaa
    18181 gttcgcgaga acggcggctg cgttgcgcct ttttcgggca tgtcctggga agtgcgcggt
    18241 aacggcgaga agccgctgct tcatctatgg tcggagcaat ttaacctcac gcgccgcgtc
    18301 ctcgccatta ccgatcattc cgagcagcgg ctggcgcttg ccgtcgagcg cttcggccgc
    18361 agcaaaccgg accggctcga gttcattcgc agggagtttg agcgcggcgc ccgcgaactt
    18421 tcacgcgagg aattccgcga ccgcctctcc cacctgcttg ccgagcagtt tccggatgaa
    18481 acaatcgaat cgcttgcggc ctctcccgat ctcgagcatt ccctctccgg caattacgcc
    18541 cggggaattc tccgccgcgg ttccgcgtac gtcgccgtgc tggccgtgcc cggcggagag
    18601 gcaacggacg cgtccgacaa tagcctcacc ttcgctctac tctggttgcg tcatgcgcgc
    18661 caatcttctt gccggggagc attaaccggc gttcgcatta ttcttcccaa gaacgcttgc
    18721 ggcatcgtgg cgcgccgtct cgccgcactc gacccgcaac tcgccgtgga gctatacgag
    18781 cacgaccccg cgctgaacat cctcgaaaag atcgatcccc ggcgcgcggg caatctggat
    18841 acctggctcg tcccgcaccg cgaatcagaa gctcttctcg atcaggcgcg gcccgcgttg
    18901 gatgccatca tcgcattggc gccacaggca gtgactctgc atcccgcggc gcaatcccgt
    18961 gaagtgtggc tgcgttttcg tggcctcccc ttcgcgcgct ggcaggatgg ccgcgttttt
    19021 ttcggaatca acgacgtccg cgaggaactc actcccgctt cacgccctgc cctgaagcat
    19081 ctactgcacg atctggaagt tcaccgccac ccgctggcca gcgatacgcg ccatccgctc
    19141 taccgcggcc agccggagcg ctggctggaa tccatcgttc acgaggacgt cacgcgcatc
    19201 gatgccatgc tcgatcagcg atttgtttac gctcaggttt ttgccaatgc cgggggcgag
    19261 cacggcattc tcgacctcct caccgtgacg cgttccggac gggtggcgat cattgagctg
    19321 aaggccaacg agcacatcca tctgccgctg caggccgcgg attattggct gcgcgttcgc
    19381 cggcagctgc agagcggaga gatcgctcgc tacggctatt ttccgggcgt cgctttacag
    19441 caggcttcgc cgctcgtcta cctcgttgcg ccagcgctgc gattccatcc gacaaccgac
    19501 gatctcctga attatctttc accggagctt gagatcgtgc gagtaggcct tgccgaaagc
    19561 tggcgacgcg gcctgcgcgt ggtcatgcgc cagtagctca ccgcggtgaa ccgctcgcaa
    19621 cggcagagcg ctgcctttat ccacagcctt tttctttctc ccggaagttt tctgcccgcg
    19681 gccgctatcc cacaggtaga atgtctcccc ggcagatcgg gtacaaaccg tgcagcccac
    19741 aaacttttcc gtcaccattc gtcaagcggg cccaatttcc ctccttgatg tatcggggcg
    19801 tctgacttcc ttcgagaccg gtgccttgcg cgattccatt tcccggctgc tgaagcaggg
    19861 ccgaaaaaac atcgtcctga atttaagcgg gctgcaatac ctggacagct ccgggatcgg
    19921 cgagctcgcg aggatttatg tttcggtcgt gaaagccagc gggcagatga aagtggtcgg
    19981 cttgtcgccg aaagtggaag agattctcaa gattacccag ctctatcagg ttttcccgga
    20041 attcccgagc gaagaggcgg ccctgctgag ttttcccgaa tccagccgca aagaaaccat
    20101 ttaagactct tatccgtgaa aaaccacgct gaaaggaaga gaagcggtta gggcattgcc
    20161 ctaaccgcct cccggggtgt gtcctcgaag tggtattggt atattagatg gccgccagct
    20221 aaaaaggttt ccttttgatc taactttttt caaaaaaatc gatttagcag cgtaaatctc
    20281 aataaaacag atacttacgc caccgttcgt cgcggtgccc gatcagccgc atcagctgtc
    20341 gggaggtgtg cagcgtaaac ggccggggtc tccgcgccag cttcagccca gcctcttccg
    20401 gcgtccggtc gcccttgctg ttgttgcacc ggtagcagca ggccaccagg ttttcccagg
    20461 tggatcgccc gccgcgcgac cgcggcacaa cgtggtccag cgtcagctcc gaacacggaa
    20521 atactcgcac gcagaactgg caggtattgc ggtcccgcag caaaatattc ttccgcgaaa
    20581 gcgcccggct ctgctgcgga atgtgccggt acgccagcag gcggatcacc gacggcactt
    20641 tgtgcgcatg cgccgcggag tgcacttccg tgttgtgcat ctcctccgcc tgcgccacgc
    20701 ccttcagcat caacaccatc gcgcgccgca ccgccgtcac gttgatcggc tcgtacgtcg
    20761 cgttcagcac cagcaccgga tcctgcatca gcgatgcacg gtgcggcgaa atcaagttgt
    20821 gggatctact gcctgcggga ccctcggagc cgggcatagc ctcgcccgcc gcttcggggg
    20881 tatagcgccc ccgggaatcc accgacatgt gccggtttgt gcccatcgcg ccttctactg
    20941 gttatcttac cactctccgg tgcttggcag ggggtttctg acggcccgcg ccacggtcag
    21001 cccaatcaca gacttggctc ccgctgtgcg caacgcgcgt gcacaggcat ccagcgtcgc
    21061 tccagttgtc aatacatcat ctaccagtaa gacgcgtaga ttgtcaactt ggctgcctgg
    21121 acgtgtggca aaagcgccac gcacggactc ccatctttcc tccaaactca gcacttgctt
    21181 gtccggccgc gcccgcgtgc gcatcagcag caccgctttg tgcggcaatc gcagcctccg
    21241 tgccaggggc ttggagagca gcgccgcctg gttgtacccc cgctcccgtt ctcgctcgcg
    21301 atgcaaaggt accggcacca ccacgtctgc cgccagcctg tctccctcag cgttcaccac
    21361 ttccgccagc cgctccgcaa accatgcccc aagcggctcg atctgctcga atttcagcag
    21421 cagaatcgcc cgcaccaccg cgtcttcata caccgcaaag ctgcgggctc tgtcgaacgc
    21481 ataggttttg tccccgcaag ccgggcagag cagcggctgc tcaggttccc gcgtcaatcc
    21541 aggcagaggc cgcccgcaaa cctcgcatac gatgctgggc acacgtttga acgacgaaag
    21601 gcactccccg caaatcggaa cccggctcgc gctcgtcaac agcctgtcgc agatccggca
    21661 tccggcagga aaaaacacgc tgacgatcgc gtcgctggcc tctgcgagcc agccccaacc
    21721 gggtcggagc ccgccgcgca cacctccgcg ccgtgctgcc gccgtgccgc tggatgagtt
    21781 cccgtccgcc acgccagcag caatctactc ttctttttgt tttaattaaa caggaaatgt
    21841 acgccgcagt aatcgagctt tctgaaatgg tcctgtagag gaggggcgga acttgtcccg
    21901 gccgggtcgg gagccccgcg ctttgaactg cgccgcgccc atcattgcag caatccctgc
    21961 tccatctttt cctggcaagc caggcaatgt ttcgcccatg gcaccgcctc cagccgcttc
    22021 tgctgcaact cttcctggca attcgcgcac actccatatt cgtccgcctg gatgcgcttc
    22081 agcgccgcgt cgatcatgtt caggatcttg cggtccgtat tggtcatccc aaagagaaac
    22141 tccttcgtat aggagttcgc cgccttgtcc gccaggtcca ccgtcgcatc gtcgtccgcc
    22201 gtgcggccct cttcctgcgt gcgcgcgatg gtcttcgtca actcttcccg ccgcacctgc
    22261 aatttcttct tgtaatactc cagccttttc ttgtccatgc ctgccttgac cttgtctgtg
    22321 cctttaatca tgctcttgtg catgcccttg tttgcatgcg actgctgctg ctctcgtcca
    22381 caacggtccc gccccactac atgctcttcg ggaccatcta aaaactccgt agtgtaggcc
    22441 gacttaacgg gagtgtcaag tttgacgccc tctttgtgtg cttcgtttct tcccagcaat
    22501 ggattttctt gtaggggcac gatagatcgc gcccttcttc ggcctttgta gcgccgggcc
    22561 ttccctcggt ttatgggggt cccgaagaat tatttcggga agcccggcat ctttgttttt
    22621 ttgtgtagcg gcgagggaat ttatcccggc ccggtcggga cctcgccagt tcttgtcttt
    22681 cgtggagaat caactcttac gaagaaaccg ccgccgaatt caacttctcg ctgccccgag
    22741 ccagccggtc atactcggcg ccagaactct ccggcagcag cacccacagc gtataaatcc
    22801 ccagcgccgt ccccagcgga aaacgcagca gcgctaggaa ccccagcgcc agccccagca
    22861 tccgcgccca gggctcgcgc tcgaaaagtc cccacgccag aaccaggtgc agcacgccga
    22921 agagccccag aaaaatcccc acggaaaaaa gcccgcccag gaaaggaatg tcccagcccc
    22981 accttccccc aaagggccat accacaccgc gtccccagcc gcgcagaaac gggaaaacca
    23041 tgctgccaaa gagcatcatc cagcagactc ccgccagccg cagaatcccg ttaatcgtcc
    23101 acagcgtcgc cacagtccgc agatgccgct gcacccgccc atcggccccc gccgccacgc
    23161 cgtaagcccc cgcaggcccc gccgcctgcc ccatccctcg acccgccgca ggaggtgtcg
    23221 ccgctccgcc ccccacaatc gccttcccac acttcgagca gaattgcgcc cccgccgtga
    23281 gctgcatccc gcatcgatcg caatacatgg tccaccccct gccagataat acgccccacc
    23341 cgccagtaag ttccacgcgt ttgagccgtc ttccgccctc ggtggcgagc ccccaccccg
    23401 actggggaat ggccttgcat ccctgcattt cctggaacgg aaagaacgac ggaattggca
    23461 gtttgcggtt tcattttggt cctcgacgag aggtgggtgt gcccccacgg ttgtgttttt
    23521 tgacgcagag tgtggaaacg attgaaaaca agaggtttga gttttttggg agtgcaaaaa
    23581 agtgcagaag agtgtggaag aatgtgaaaa gacaggggat agttcaaaaa acgttggcac
    23641 gtcccaacgt tggaaggtct gaacgttgaa ggtaaagaag agatggaggg agaatggcgc
    23701 gggctatttt cgcggtggtg gagtcgagga ttcacgactc atgataccgt agattatagc
    23761 atttgtcaag tactaataaa gtatttatat tgaacaagtt acggcatctt tcgaatgggc
    23821 gcgtaagtga aagaaaacaa aggggtagag ctcactgggg gcggcgcaag ttctcaagag
    23881 ctacggaagg cggaggccgg gtcggcggtg agggcggggt tagcggtgct ggaggaagtg
    23941 agcgaagcga agaattgccc accctttgca aaaaccaagc cgcaaagggt agggcaccca
    24001 gatcgtccga aagggaaatg gctggcccac ccgcctgaag aaactacatg aatccacgga
    24061 aaattgactg ccccctaagt gcctgaaagg taacgcaggg tcggagggat tctttttctc
    24121 gcgaagcgcg caaggatatg gttagcggca ggtcacaagg cggtgccacc cgagcttagc
    24181 cagaggctga caagaagaac agcagatcgg ggaattctgg atgtgctgca tctctctgaa
    24241 atggcgcacg aacaacttcc aacaggcccg cctgaccaga cttatttggc aaacgctcaa
    24301 acgcactaca aatggtatcg gaccttgctg cgacatagac acaagactcg ggtccaaatg
    24361 atttgttcca acgaatcgtg tgttcatctt cgcaattctc cgacgtaaaa aaagactgtc
    24421 ggcgatactt ggggcttggc acttcatcgc tggcgcgttt taagtcgctg aacaaagaca
    24481 cctggaaaga gcccgccaat ttgcgttcct catcaatccg acgtcgatac ctcaaaagcc
    24541 ccgcgactgc cacctcgggg tgtgctgtcg caagacgaaa cactgttgcc tcgaacgagc
    24601 taccaaattt ttcagccagc tcttcagtcc tggtcaaact gaaaaaatta caaccttccg
    24661 cccggaattc ttcgataggc atcaatagct cggatgcagc caagtcgcac agcctttcga
    24721 gttcgttcgc ttcacgcgag tcagacgcgg tcagatcccg gaacctagct cctgttagcg
    24781 tcctcgggaa aaatgtgtgg ctgatctcat gcgcgattga aaataccact ctgcctggtg
    24841 atcgcgaggg attgtagaaa atctgacctt tgcttcggcg gacattccca ttaggaatga
    24901 gaacagcgtc gcgcggctcg gctctcctct gttcgatatt cataggcgaa atcgtgaggc
    24961 cacgcagaga cgcaataatt tcgagtcgct tgaagggatc agtggggccg ccctcgtaag
    25021 aacgatattc ttggttcagt tttcgcgctt ggctcacaac agcagatcgg ggatctacca
    25081 actcccccgt agcgcgaatc agagatataa catcagggtc cgaataccta cgtccaccga
    25141 ctcgaacagt ttccattgcc taatttcgtg gctttctttg gcccaacata ttcagtgttt
    25201 ctatcagata gagccacccc tccttatctc ttggtctatt tcctcgaaag cttacctgtg
    25261 ccagcttctc gacatcatca tcggacagat tcagttcgag ctgcagatct ctgagccccg
    25321 acggcagctc cgattccccg cgtttcggcc gtccaccttc gttccgcgcc ccgctctcct
    25381 gcaggagctc acttaacgta accccgagag caacagcaac tgaatagatg tactgaatgt
    25441 ttggcttacc agccgacccg ttttcgaggt ctgaaagata cgcctttgaa agcccggatt
    25501 ctttcgcgag atccgcaagg gaccagtttt ttcctttcct gaggaatctg atgcgatacc
    25561 ccagtcttgt gagatctatg ctcttgctgt ccattatata caaccggttg caaaaaagtg
    25621 ttgatccaac tcgccccctg cgctatccta ggttgtgaaa atacgaccgg tcggcattcc
    25681 cggatattta accgcagacc tggccgaata gcaagtcgtc aagtgggggc tctagcttcc
    25741 acaggcgatg aaactagcca agcacaggag gacagtcgtg aggaagaaag tcgaattcga
    25801 cgctcacaaa gttgttaaga aaccaacgga agtgggattt acgaccagaa atggaaagga
    25861 agttgatttc gtcgcgagga agaacaccaa agtgcctgtt catgtgaggt tcaaagcaaa
    25921 cgtgaagtag tgtctaccgt tcgtgggatc aaagaagggg gaacaaatgt ccaatagtgg
    25981 actaagaggc ccatatccgt taaccaaaac gggagtcgaa gacgaagtta ctaaggcgtt
    26041 gcccggagct tatgtcctag ggaggtccga cacaaccgca tttcgcattt cttacgtcgg
    26101 tcgtgcggac gacgatgtca gaaaaagaat tagggatcac gtccctgagc catatccgca
    26161 atttaagtat gagtattatc cgtctccaag agctgcgttc gaaaaggagt gcaatctcta
    26221 ccacgacttc acaccgcccg ataacaaagc ccacccggct agacctgccg gcgcaaattg
    26281 gaaatgccca gtatgtagca ctttcggcta atttcagttc tgtgttcata actaagtttc
    26341 acaactccac tgacgtaggg acacaggtgg agtgacctgg taacgagtga cttgcggtag
    26401 atagggaatc aagatgacag accaagaacc aacacgcgtc gactcagaac cacagacaag
    26461 aattgtcaag cccataaggc gattcgcagc tctactcggc ttcttagcga aggcggaaat
    26521 ggacaccatt ttccatcaat agccgttcga gacaattgac ggtgcggatc ccattgaatt
    26581 gtggcgacag tacgaaagcg gacgggcaaa actttcagct ctcgaaccag gtctgcttga
    26641 agccttgccg ggttctctca ccggtctgat cggggcgatc aaagagaggc agacctacaa
    26701 gcgatactac gaggcagtcg cggattactc cttcatggca gtgccgatag catctctagt
    26761 ggcgccgcag tggggcgcgg acctagattt cgtcgacgaa atagtttctg catcaagcaa
    26821 ggatatgaat attgaacagc agctcctttt tgcaatgtcg gaagggaaaa tcaccgaacc
    26881 aatcgttact ggcaatcagg tatggtttac cagccctagg agagatctgt tcgctcatcc
    26941 ggtgccgacc gtgagagaga ttggggaagg tgagtttgaa atcgtcattc gcgccgcaag
    27001 tcggccaaac tacatacagg ttgccgccgt tgagaatagg ctactcttga caaacggcgt
    27061 tcataaggtg tgcgcgctcc acaagatggg ctacacacat tgcgtatgcg tcttccgacg
    27121 agccaatgac ctcaccgaag ccggtatcga tcaacggcaa accacccttt ttcgcgatgc
    27181 tgtctttaag agtatgcggc ctgccttggt cacagacttc ttggaaccgc tagcggcggc
    27241 gcccctaaag atgcgcgcaa tgtatcaggt tcttcaaata gcgttcagcg ttgggacgat
    27301 taccgttccc gccctcccaa agaatcattc cacccagaag cctcgagtgg gtgcggcagc
    27361 cacgaactgc taggacggtt tcgggtgccg caggcttcag ctttaagcgt cctggttttc
    27421 attttctgtt tagcctccgg ctccgttttc acggtatcac aggcacaatc agcgccgaag
    27481 gatgcgcctt gtcgtggtaa accacattcg ttgctttgag catgcgcgtt gcgcgcgctt
    27541 gctcttcgcc ggtgttcagg ttgcggtcga agcgcggaaa attgctgctg gaaacttcca
    27601 gccgcagctt gtgccccgcg aggaacacat tgctcgtggc ccacaaatcc acggtgatgt
    27661 gatacgtctc gcccggattc gccagctccg gtttctcttg cgaattgcgg taacgcagcc
    27721 gcaggattcc gctcgtcaag ttctgcgcaa acccattcgg ccaaacgtcc acgagcatcc
    27781 ccgtgaagtc cgtatccacc gccgagctgc tcacatacaa atccagcgag accggcccgg
    27841 tcacttcggt gtccttcgca aacgccggcg tcgtgtacac caacacatcg ccgcgcgctt
    27901 ccgccggccg ctgatcttct ggaccaatcc ccgttggcag cgctccgcag cacaacggcc
    27961 cgccaatcgt tggcgccgcg tcgctcggat cgtaaacgta ctggtcggcc ttctcttccg
    28021 cgggagccac ggtactcaaa gccccgttgc ccgcgatgcc gttcgccgcg cctgccgaat
    28081 gcaggtaata cttcgtattt ttcgcgcgcg ccagcggcca gtcgtcttct tcgcgccact
    28141 cgtttttccc catcacgaaa atcttcaccg gcttttcttt ctcgacgccg ttcgtttctc
    28201 ccttcagcag ccagtcgtac caccgcagcg tcaactcatc tccatccgcc ggcaatttgc
    28261 tgccaaactc caccgcgcct acttttccag tgctcgattg tccggcgtgc cccccgaccg
    28321 tgaccaccag ccgctgccct ttgcgcgccg cttccgttcc gccttccgtt ttcagccgca
    28381 cgtagttctt cagcgagccg ccaagaaaaa tgtcgtacca cgccgcgaaa ctcagcaccg
    28441 gtacttcgat ctgcgcgtag tggtcctcga tcgacaactg cttccaatac tcatcgaaat
    28501 tcggatgcgc cagccaatcc ttgaaatacg gcgcgatccc ttccgcggaa ggcgcttcca
    28561 gcacggggta ggagatcagc ggcagcacgt tggtgccgcc tagcgcgtcg gccttctttt
    28621 ccgcgcgccg ccgcatggtg tttgtcgcca aacctgtcga ccacgattcg ttgaaccact
    28681 gctcgaacgc tccgccttga tacgtccagc cgtcgtgata attcgacgcc gttacagtgg
    28741 ggcaaatccc cgccaggtgc ggcggcttcg cgagcgccgc caggaattgc gtcgcgccca
    28801 cgtacgatcc gccaaacatt cccacctttc cattcgagta gggaagcgcc gccgcccact
    28861 ccacggtgtc gtagccgtcc tgcgactcat acttgaaggt gtaccactcc ccctccgact
    28921 cgaagcgccc gcgcacgtcc tgcgcgatca ccacatatcc gcgcgccgca gccttgagcc
    28981 cgaagcccat gctccccgtc ttgtcgtacg gcgtgcgcac cagcaggacg gggtaccgcc
    29041 cctccgtctt cggccggtaa atgtccgccc gcagcgtcac gccgtcgcgc atcttcgccg
    29101 ccacgttgcg ctccaccgtg acttcgtgga tttccgccgc cacgactggt ggcgccgcta
    29161 gccccaaagt caggacgaaa gcggcaaaaa acagaacggc ttgctcagca agcttcaagt
    29221 tctcttgcag atacttttgc atgcgaatgc ctcccacgag gtttactcgg cggttcatta
    29281 cgagtggtgg agcctaggct gagtccccgc cgctgtcaag gcgcgaccgc ctttggggag
    29341 ctacattttc accggacaga ggctggggag gggttccaaa agaaaagcga aggatttgca
    29401 acgatttgcc ttctttggga agagaatggg gcaacgggaa agattcttgc atacgcgtgg
    29461 tctcaaccaa tttcagggga gtccgtatcc tattgtaaat ggaaggttaa tactaattaa
    29521 caggattctc acagtctccc aatttggctt cagaattgca ctatacaggt ggtacgtcca
    29581 agcgaggacc tttgaaaaaa ctcttcctgt tagcctgttt gatcgttctg gtagcgccct
    29641 atagcgcatt tgccggccac aagcgccaca ttcgagcgga cgaaatggct ggaatcggtg
    29701 tgggcgcggc ggccctggtt ggtgcggcgg gctatctttt gcttcgccgg cgccactcgg
    29761 cttaattcgc gacgctcgtc gtggactaag tccgttaaag tatcgtcgga ggttcgagag
    29821 gaatagtgtc tccggcgagt acaccagacc cgagccttct ttcccgcgga ttctttcgaa
    29881 cggggagctt cgcagctccc cgatttcttt tgctggctct ggccctcacc ttggttgcta
    29941 ttgcggcaga tcagttcgcc gcgccgattc tccacagcac ttctccgctt tgggccacca
    30001 ccgcatgctt gttgctcgtt tggcgacgcg acaaattcac ctcctctgcc gacgatccac
    30061 cgctcgagct ttccatctcg atggggcgtc tggcggcgtt cctcgcagcg catagcctac
    30121 taattctttc agcgcgctcc ttgagcggca cgtttcaagc tgtttccggt gtgatgacct
    30181 tcgggggaac tcttgttgca gtgtgtaagc tgggcgtttt ggcgcccacg ataattcttt
    30241 tttcgcccgc ggtgtggaaa aaaatcgcga ccatttactt tcccgaagcc atcgccggtc
    30301 ttgtagtcct gttaacattt tttcccagcc gcacactgga gacaatctgg ccctggtacg
    30361 ggcaggtgct gggccgattt gttcacacgc tggcgcggat cttcgtgccg aggctcgcgt
    30421 acctcgccga ttcgaatcca actctgagcg gtcccgaact ggacgtcact atcgttccgg
    30481 agtgttccgg aatcaacggg ctcgaactct acgattacct ttttggtgta gtggcgtttt
    30541 tcgattggaa tcgccttcga aagggccggg ccctgacggc atacttcgcc ggcctctttg
    30601 ccatgcttct cggcaacgcc attcgaatca cttcttttgt ggtgctcggg aatcacggct
    30661 ttgcggaaag cgtctcgcga tttcatattt ccgcaggctg gattttcttt tcggtcgtct
    30721 ttctcgttta tctttccatg acgtacagtt ggatgctcgg caagagagat accgccgtga
    30781 aacatcagca gatgagctag taccaccctt cacgggcgca gcgattacac tcatttcctc
    30841 tacacgcacc aatagaaatc cggccaacat tcgaacgaga cttttctcgc aataaatcct
    30901 ctaaatttag cgatgttacc tctcgaattt cacgtagctg agaagcattc cagaaacgtt
    30961 tgaggtggct ttgctcccgg aacttcgtct ttcactttag tcccggatcg aggttccacc
    31021 gttccttggg cagcttccgg ttgacggcca tcaggaagcg agccgccgag gacggactgt
    31081 ggtgagttac aggtccgggc gggagaaaaa gatgtactcc gcccctcgga gcattctggc
    31141 cgtcgctctt ttttttatgc cgagcttttc cggaacggtt gcgttcccgc aggccgtcaa
    31201 attcatccgt cacgcggctg atcctctgcc gctcgccaac ttgcctgttg gccgggcacc
    31261 ttgcacgaaa gactcgggga gcgcagtctc cgctccgtac gggagtacgc gggcaaccgg
    31321 tgacgcagg
//
LOCUS       DASYVO010000152         4011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_227940, whole genome shotgun sequence.
ACCESSION   DASYVO010000152 DASYVO010000000
VERSION     DASYVO010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227940"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1642
                     /gene="uvrC"
                     /locus_tag="VGR03_05125"
     CDS             <1..1642
                     /gene="uvrC"
                     /locus_tag="VGR03_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV1993692.1"
                     /translation="NEREALALEHNLIKQYRPKFNVVLRDDKTYPYIKFTAAEKYPRV
                     YFTRRIKKDGSLYFGPYFPSSLARRILHFVHKRFLVPSCTVDLTRSHPRPCLQYYIKR
                     CLGPCVAGLTTDERYAEAARDVRLFLEGRRHDLIKSLEERMAAAAEKELFEQAAAYRD
                     LLRTLEDIEERQRIAAAQGDDTDVLAYYAEPPLVAADLFHLRGGRVVDRREFYWEELE
                     EFDPQEFVTSLLKQLYLEAEYLPKAIHVSADFEDRELLEATLTERAGHKVEIFTPQRG
                     TKRAFLDLVESNAKHSFEQRFRVLKPTSKAIGEALQNALNLAEEPHRIESFDISHIQG
                     TDTVASMVVWEKGRMKKSDYRKFIIRGDAGRGEKGANENPRGQNDDFAGMREAVSRRY
                     RRLQEEKKELPGLILIDGGIGQLHAAAQALESLQIINQPVASIAKKEEILYVLGQEDE
                     PIVLERHSPVLHLIQQIRDETHRFAVTFHRQRRGKRQTETVLSEIPGVGAKTAQKLLR
                     EFGSVANIQRAGLEKLSSVISRKSAEKILSQLGASTTQ"
     gene            1721..2008
                     /locus_tag="VGR03_05130"
     CDS             1721..2008
                     /locus_tag="VGR03_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV1993693.1"
                     /translation="MADNVGSKVTYFLVGLGVGALVGILFAPKSGEETRDFLAKKADE
                     GKDFAQKKARELRERADELIERSKEVAVRKKDSISAAVEAGREAYLRESKS"
     gene            2067..2582
                     /locus_tag="VGR03_05135"
     CDS             2067..2582
                     /locus_tag="VGR03_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEV1993694.1"
                     /translation="MQFWLEFFSIVAAIALVVQVVILTMLFFQLRRTTENVNRLVGDL
                     HSRVGPILTRVQILLDDTQPKISSMVNDASHIVYLARGQAQKMDRVFTDAADRLRGQL
                     VHADRILTGTLEAVEDAGSKFKHSFWRPVQKASALVQGIKVGLDLLRSRRKSGRRGDE
                     PREQQEEELFI"
     gene            complement(2697..3848)
                     /gene="mnmA"
                     /locus_tag="VGR03_05140"
     CDS             complement(2697..3848)
                     /gene="mnmA"
                     /locus_tag="VGR03_05140"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV1993695.1"
                     /translation="MSTATQFDTLPESAETQRGLVVVAMSGGVDSSAVAALLQQQGRA
                     VVGLTMQLWNQRRLPELQGDGPAQHRCCSLDDVYDAKAVAQHLNFPHYVVNFEEQFEA
                     RVVRPFVDQYLAGRTPIACTNCNTDVKFEPLLRMARQIGAERLATGHYARIRKNGDTG
                     RWELLRACDESKDQSYFLWGLSQGQLSRSDFPLGELTKEEVRALARRANLPVAEKPES
                     MELCFVPTGNYVQFIQAYSQERGISLQNAEGEIVNENGDIIGRHNGVHGFTIGQRKGL
                     GFAAGKPLYVLSIDPEKNRVVVGEDEVLRKTVCEVKGVNWISYEKPSAPLRAFAKIRH
                     KHEPAPATIEPLNAATARVTFDTPQRAITPGQATVFYDGEVVLGGGWIR"
BASE COUNT          843 a         1017 c         1331 g          820 t
ORIGIN      
        1 gaacgaacgc gaagcgctgg cgctcgaaca caacctcatc aagcagtatc gcccgaaatt
       61 caacgtggtg cttcgcgacg acaagactta tccgtatatc aagttcacgg cggcggaaaa
      121 atatcctcgg gtgtacttca cgcggcgcat caagaaagac gggtcgctct atttcggccc
      181 gtattttcct tcgagtctgg cgcggcggat tcttcatttt gttcacaagc gattcctcgt
      241 tccttcctgc acggtggatt tgacgcgcag ccatccgcgg ccttgcttgc agtattacat
      301 caagcggtgt ctggggccct gcgtggctgg gctgacgacg gacgagcgct atgcggaagc
      361 cgcgcgcgat gtgcggctgt tccttgaagg tcggcggcac gatctgatca aaagtctcga
      421 agagcgcatg gccgcagcgg cggagaagga actgttcgaa caagcggccg cttaccgcga
      481 tttgttgcgg acgctggagg acatcgaaga acggcagcgc attgcggctg cgcagggaga
      541 tgacactgac gttcttgcct attacgctga accaccgctg gtcgctgcag atcttttcca
      601 tttgcgtggc ggacgtgtgg tggatcgccg ggaattttat tgggaagagc tggaagagtt
      661 tgatccacag gagttcgtga cttctcttct gaagcagctc tatctggaag cggaatattt
      721 gccgaaagcg attcacgtct cggcggattt tgaagatcgc gaactgctgg aggcgacgct
      781 gacggagcgc gcggggcaca aagtggaaat cttcacgccg cagcgcggga ccaagcgggc
      841 tttcctcgat ctcgtggaga gcaacgcgaa acattcgttc gaacagcgat tccgggtatt
      901 gaagccgacg tcgaaagcga ttggcgaggc gttgcagaac gcgctcaacc tggcggagga
      961 gccgcatcgc atcgagagtt tcgacatttc gcatatacaa ggaacagaca cggtggcgtc
     1021 catggtggtt tgggaaaaag ggcgcatgaa gaaatcggac taccgcaaat tcatcattcg
     1081 tggagatgcg gggcggggcg aaaagggagc caacgaaaat ccgcgcgggc agaacgacga
     1141 cttcgccggc atgcgcgaag ccgtaagccg ccgatatcgc cgcttgcagg aagagaaaaa
     1201 ggaacttccc gggctgatcc tgatcgatgg cggcatcgga cagctgcacg ccgcggcgca
     1261 ggcgctcgaa tcgctgcaga tcatcaatca gccggtggcg agcatcgcga aaaaagagga
     1321 aatactttac gtcttggggc aggaagatga accgatcgtt ttggagcggc attccccagt
     1381 gctgcacctc attcagcaaa ttcgcgatga gacccaccgt tttgcggtta cgtttcatcg
     1441 gcaacggcgg gggaaacggc agactgaaac cgtgcttagc gaaatccctg gggtaggcgc
     1501 aaagacggct cagaaactat tgagggaatt tgggagcgtt gcgaatattc agagggcggg
     1561 gctggaaaaa ttgagcagcg ttatttcgcg gaagagtgcg gagaaaattc ttagccagct
     1621 tggagcctca acgacgcagt aaccagttgg caggccatat agcggttagg cctcattgcg
     1681 cgcctcgcag tgctatgcta cggtttccgt ggaggttgct atggcggaca atgttggttc
     1741 caaggtgact tatttcttgg ttgggttggg agttggagcg ctcgtcggta ttttgtttgc
     1801 gccgaagtcc ggggaagaga cccgcgattt tctggctaag aaggcggatg agggcaagga
     1861 cttcgcccag aagaaagccc gcgaactgcg ggaacgcgcc gatgaattga tcgagcgcag
     1921 caaggaagtg gcggtgcgca aaaaggattc catctcggcg gcagtggaag ccggacgcga
     1981 agcgtatctg cgcgaatcga agtcctaagg gctcggtaag ggagtagcac ggggttccgt
     2041 ctcttagtgg ggcgggtggg gcagctatgc aattctggct cgaatttttc agcatcgtcg
     2101 cggcgatcgc actggtggtg caagtggtga tcctgacgat gctgttcttt cagcttcgcc
     2161 gcacgacgga aaatgtgaat cgtctggtgg gcgatttgca ttcgcgagta ggacccattc
     2221 tgacgagagt gcagattctt ctggacgaca cgcagccgaa aatctccagc atggtgaacg
     2281 atgcttcgca catcgtgtat ttggcgcgcg ggcaagcgca gaaaatggac cgcgtgttta
     2341 cggacgcggc ggaccgcctt cgcggacaat tggtgcatgc ggatcgcatc ttaaccggga
     2401 cgctcgaggc cgtggaggac gcgggctcaa agttcaagca cagtttctgg cggccggtgc
     2461 agaaggcgtc ggcgctggtg cagggaatta aggtggggct cgatcttttg cgttcgcggc
     2521 gcaagagcgg ccgccgcggc gacgaaccgc gcgaacagca ggaagaagaa ctttttatct
     2581 gaactaaagg acgcggcgcg aaacccgagg ggacagacat ttttgtctgt ccttttttgt
     2641 ttttgcgaac tcccaccgga gtaaagagat accgagcgcc tgtatcggag ctggcttcag
     2701 cggatccagc cgccgccgag gacgacttcg ccgtcgtaga aaacagtggc ttggccaggg
     2761 gtgatggcgc gttgtggagt gtcgaaggtg acgcgcgcgg tggcggcgtt cagtggctcg
     2821 atggtggcgg gtgcgggctc gtgcttgtgg cggattttgg cgaaggcgcg aagcggggcg
     2881 gatggctttt cgtaggaaat ccaattcacg cctttgactt cgcataccgt cttgcggagg
     2941 acctcgtctt cgccgacgac gacgcggttc ttctccggat cgatcgagag aacgtagaga
     3001 ggcttgccgg cggcgaagcc gaggcccttg cgctggccga tggtaaaacc gtgaacgccg
     3061 ttgtggcggc cgatgatgtc gccattctcg ttaacgattt ctccttctgc gttctgcaag
     3121 gaaattccgc gctcttgtga atacgcctgg atgaattgca cgtagttgcc ggtgggcacg
     3181 aagcacagct ccatgctttc tggtttctcg gcgacgggca aattcgcgcg gcgggccagg
     3241 gcgcgcactt cttccttggt gagttcgccg agagggaagt cgctgcgcga aagctgtccc
     3301 tgcgagagac cccagagaaa gtaggattga tccttggatt cgtcgcacgc gcgcaacaat
     3361 tcccagcggc cggtatcgcc atttttgcga atgcgggcgt agtgaccggt ggcgagacgc
     3421 tcggcgccga tctggcgcgc catgcgcaaa agcggttcaa acttcacatc ggtgttgcag
     3481 ttggtgcagg cgatgggcgt gcggccggcg agatactggt ccacaaaggg acgtacaacg
     3541 cgcgcctcaa actgttcctc gaagttcacc acgtaatgcg gaaaattcag gtgttgcgcc
     3601 acggccttgg cgtcatagac gtcatcgagc gagcagcagc ggtgctgcgc ggggccgtcg
     3661 ccctgaagtt cagggaggcg tcgctggttc cagagctgca tggtgaggcc gacgacggcg
     3721 cggccctgct gctgcaaaag agcggcgacg gcggaagagt cgacaccgcc ggacatggct
     3781 acgacgacga ggccgcgctg ggtttcggcg ctttcgggga gggtgtcgaa ttgggtggcg
     3841 gtgctcatgc tagttccatt gtatcgggca aggtctccat ttgcttgcat cgtaatgcaa
     3901 agggcgcagc atcccgacaa ggtcgggaca ggtgctgcgc cactacaaag accggaccac
     3961 agtgaggcgc gagcatcatt ggtgggcagt ggcctctttg cgataggtgg g
//
LOCUS       DASYVO010000153         5631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_173196, whole genome shotgun sequence.
ACCESSION   DASYVO010000153 DASYVO010000000
VERSION     DASYVO010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173196"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VGR03_05145"
     CDS             complement(<1..412)
                     /locus_tag="VGR03_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993696.1"
                     /translation="MNAEHVDRRGVLAIAFAGLLAGGAYAQSPDPTPQQQDIQSDKKD
                     IRNDKKDLSKDRADRNADQRDINHDKNDLSKDRADRNADQKDINHDRTDLNKDRVDRN
                     KDQRDINKDKGQLASDVQKYGANSTQAQADRKDLH"
     gene            complement(550..957)
                     /gene="nusB"
                     /locus_tag="VGR03_05150"
     CDS             complement(550..957)
                     /gene="nusB"
                     /locus_tag="VGR03_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV1993697.1"
                     /translation="MSLRTKSREFAMQMLFQWDMSQQDFTKLEVKFWKNAKAADKTRA
                     FANKLFEGAAKDVAILDALIVGHSDNWRFERLAAIDRAILRLAIHEIKAGETPAKVVL
                     NEAVDLAKKFSSEESGAFVNGVLDAIHKSLKIK"
     gene            complement(959..1486)
                     /gene="ribH"
                     /locus_tag="VGR03_05155"
     CDS             complement(959..1486)
                     /gene="ribH"
                     /locus_tag="VGR03_05155"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV1993698.1"
                     /translation="MADKLKPGKISGELMAAGFRFGIVVSRFNSFITERLLAAAVDAL
                     ERSGAASKDVDIVHVPGAFELPLAAKKLAATGKYDALIAMGCVLRGETTHYDYVCSET
                     ARGLQLAQMDSGLPIIFCVLTCDTLEQAIDRAGLKGGNKGFEAGLAAIEMAQLSRKLR
                     AAHLKPAPAGKPRRK"
     gene            complement(1594..2376)
                     /locus_tag="VGR03_05160"
     CDS             complement(1594..2376)
                     /locus_tag="VGR03_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV1993699.1"
                     /translation="MGYENILYEKKDGIAYITFHRPKVLNALNNKTIEELRQALVDAR
                     DDDSVRVLILTGAGEKAFVAGADISELARRTPVDGKEFSVSGQGVAHLLETMGKPSIC
                     AINGFALGGGCELALCCSIRIASRNAKLGQPEVKLGIIPGFGGSQRLARLCGKGVAHE
                     LCLTGEMIPAEEAQRIGLVNHVYEPVELLPAAEAMARKIIANAPLAVKYTMEAIERGI
                     EMPQEEGLFLEATLFGLVCATEDMREGTKAFLEKRPPQFKGK"
     gene            complement(2482..3363)
                     /locus_tag="VGR03_05165"
     CDS             complement(2482..3363)
                     /locus_tag="VGR03_05165"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEV1993700.1"
                     /translation="MTIRKVGVVGCGLMGSGIAQVAAAAGFETVVREVSAELVDKGLK
                     GIEKNLNRLVEKGTITEAVKGEIRGRLKGTTSIDDLKNCDVIVEAIIEQLPVKRELFT
                     ALDAICTPSTIFASNTSSLTITEIAAATKRPQRFVGLHFFNPVPVMKLVEVVRTIATE
                     PAVYEEMVAFGTKLGKTPVRAHDSTGFIVNRLLVPYLLDAIRALEEGVGSIEDIDNSM
                     KLGCGHPMGPLTLLDFVGLDTTFYISQIMFDEFKERRFAAPPLLKRMVLAGWNGRKSG
                     RGFYDYSDPGNPKALKF"
     gene            complement(3583..4773)
                     /locus_tag="VGR03_05170"
     CDS             complement(3583..4773)
                     /locus_tag="VGR03_05170"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEV1993701.1"
                     /translation="MFPTDVVIVAGARTPMARYTGAFSEVSAIELAAHASREAIRRSG
                     VDPGEFDHAIFGNVMQTSADALYGARHVGLKAGLKIETPAVTVNRLCGSGIEAIAQAA
                     QRLLLGEANMVLAGGMENMTQAPFVIRGARTGLKLGGGALEDSLMAGLTDTYCGLPMA
                     LTAEKLAEQHGITRKDADAYALRSQQAAEAAYRACRIKEELVAVEVKQGKKTIVVSED
                     DHRRPETTMESLEKLPPSFKKDGIVTAGNASGIVDGAAAVVVTREKIAKERGLKPVGR
                     IVSWAVAGVDPSIMGIGPVPSSQKALKLAGLTLEQMDRVEVNEAFAAQYLAVERVLGL
                     NREKTNVNGGAIALGHPLGASGTRLVITILNELRRNGLRYGLATACIGGGQGIAMIVE
                     SLQD"
     gene            complement(4938..>5631)
                     /locus_tag="VGR03_05175"
     CDS             complement(4938..>5631)
                     /locus_tag="VGR03_05175"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEV1993702.1"
                     /translation="TALGVMITDKETQMPAGMLIVGHAEAHKWKPNEAYFLQAIGDQM
                     LMSVSHTRLRSLVRRMGVSDERTGLLSRSSYQNCLLTEADRARTQGTPLALTILQIDR
                     GAELLRQQGESPLERFLEQLARSLQPIVRQNDVAVKYTSWSLAFILPDTTLAGAQNLA
                     DKLRRAASGLRPPWDSTQITLSAGIVEAVAKHEYDSEDIVTDLINRAEFSIEEARKRG
                     GDTVVALETPKL"
BASE COUNT         1063 a         1920 c         1338 g         1310 t
ORIGIN      
        1 cgtgaagatc cttgcggtcg gcctgggcct gagtgctatt ggcgccatat ttctgcacgt
       61 cgctggcaag ctgacccttg tctttgttga tgtcgcgctg gtccttgttg cgatccacac
      121 ggtccttgtt caaatccgtg cgatcgtgat tgatatcctt ctggtccgcg ttgcggtcgg
      181 cgcggtcttt gctcaagtcg ttcttgtcgt gattgatatc gcgctgatcg gcattgcgat
      241 ccgcgcgatc cttggacaaa tctttcttat cgttccgaat gtctttcttg tcgctctgga
      301 tgtcctgctg ctgcggagtc ggatcagggc tctgggcata cgcaccaccg gcgagcaaac
      361 cggcaaaagc aatcgcgagc acacctcgac ggtccacatg ttctgcgttc attgtgttct
      421 ctcctattga gtcgttcctg gagtcaccac cacacttaca aaatcaaaca ccaggagcgc
      481 cggaaagttt cgctgccccg cgcacgggct tgtatggaag gaattgaaag aagcacaaat
      541 ggagtataac tattttattt tcaatgactt atggatggcg tcgaggaccc cgttcacgaa
      601 cgccccggac tcttcggagg aaaacttctt cgccaaatcc acggcttcgt tcaaaacgac
      661 cttggcaggt gtttcacccg ctttgatttc atgaatcgcc agtcgcagaa tcgcgcgatc
      721 gatcgccgcc agccgctcga agcgccagtt gtcagaatgc cccacgatca gtgcgtcgag
      781 tatggcaaca tccttcgccg caccttcaaa tagcttgttc gcgaaagcgc gcgtcttatc
      841 cgccgccttg gcgtttttcc agaatttcac ttccagcttg gtgaaatcct gttggctcat
      901 gtcccactgg aacagcatct gcatcgcaaa ctcgcgggat tttgtgcgga gggacacgtt
      961 acttgcgccg cggctttccg gcaggcgctg gtttcaaatg agcggcgcga agcttgcgtg
     1021 acagttgagc catttcgatc gcggcaagcc ccgcttcgaa tcccttgttc ccgcccttga
     1081 gtccggcgcg atcgatggcc tgctccaggg tgtcgcaggt cagcacgcaa aaaataattg
     1141 gcaatccgct gtccatctgc gcaagctgca atccgcgcgc agtctccgaa caaacgtaat
     1201 cgtaatgcgt ggtttcgccg cgcagcacgc atcccatggc gatgagcgca tcgtacttcc
     1261 ccgtcgcggc cagttttttc gcggcaagcg gcaattcgaa cgctcccgga acgtgcacga
     1321 tatcaacgtc tttgctagct gctccgcttc gctccagcgc gtccaccgcc gccgcaagaa
     1381 gccgctcggt aataaaacta ttgaagcggc tgacgacaat gccaaagcga aaaccagccg
     1441 ccatcagttc accgctaatt ttcccgggct tcaatttgtc ggccattgct cctgcgcgtc
     1501 gctcccgctt ctccaattcc tttacttcct ttacttcctt tacttccttt atttccttta
     1561 tttccttaac ttcctttacc tcatttcctc tctttacttc cctttgaact gcggtggcct
     1621 cttctccaaa aatgccttcg tgccctctcg catgtcctcc gtagcacaga ccaggccgaa
     1681 tagcgtcgct tccaggaaaa gcccttcttc ctgtggcatc tcgattcccc gttcgatggc
     1741 ttccatggtg tatttcactg cgagtggcgc gttggcaatg atcttcctcg ccatggcctc
     1801 cgccgcgggc aagagctcta ccggttcgta tacgtgattc acaaggccaa tgcgctgcgc
     1861 ctcttcggct ggaatcattt cgccggtgag gcataactca tgcgcaacgc ccttgccgca
     1921 aagccgggcg agccgctgcg agccgccgaa ccctggaatg atgccgagtt tcacctcagg
     1981 ctgcccgagt tttgcgttcc ggctcgcgat tcgaatcgag caacaaaggg caagctcgca
     2041 ccccccgcca agcgcaaagc cgtttatggc gcagatcgaa ggcttgccca tcgtttcgag
     2101 gaggtgcgca acgccctggc ccgaaaccga aaactctttt ccgtcaaccg gagtgcgccg
     2161 ggccagttcg ctgatatccg cgcctgccac aaatgctttt tctcccgcgc cggtcaggat
     2221 caggacacgc acggaatcat catcccgcgc atcaaccagc gcctgacgca attcctcgat
     2281 tgttttgttg ttcagcgcat tcagcacctt cggccgatgg aaggtgatgt acgcaattcc
     2341 gtctttcttc tcgtacagta tgttctcgta acccatctct tccctcttct cagctccgcg
     2401 ttgctcggcg tacggcacct gccccggccg gggcggggcg tctctgcgtt atctgtttct
     2461 ttaattttct tttttctcgc cctagaactt cagcgccttc ggattccccg ggtcggagta
     2521 atcataaaac cctcgtcccg acttcctccc gttccacccc gccagcacca tgcgcttcag
     2581 caatggtggc gccgcgaatc ggcgttcctt gaactcatca aacataatct gcgaaatgta
     2641 gaaggttgtg tccagcccca cgaaatccaa cagtgtcagc ggccccatcg ggtgcccgca
     2701 gcccagcttc atggaattgt cgatgtcctc gatggaccca acgccctctt cgagcgcgcg
     2761 tatcgcatcc agaagatatg gcaccagcag ccgattcacg atgaagccgg tgctgtcatg
     2821 cgcgcgcacc ggcgttttgc cgagtttcgt tccgaacgcc accatctctt cgtaaacggc
     2881 aggctcggtg gcaatggttc gcaccacctc gaccagcttc atcactggca cgggattaaa
     2941 aaagtgcaag ccgacgaaac gctgcggcct cttggtcgcg gcagcgattt ccgtgatcgt
     3001 caaggaggac gtgttgctcg caaagatcgt cgacggcgtg cagatcgcgt cgagcgcagt
     3061 gaaaagttct cgcttcactg gcaactgctc gatgatcgct tcaacgatga cgtcacaatt
     3121 cttcaagtcg tctatggaag tcgtgccctt caatcggccc cggatttcgc ccttcacggc
     3181 ttcggtaatc gtacccttct ccaccaatcg attcagattc ttctctatgc ccttgagacc
     3241 cttgtccacc agctccgcgc ttacttcgcg caccacggtc tcgaatcccg ccgcagccgc
     3301 cacctgcgcg atccccgaac ccatcaggcc acaccccacg acacccactt ttcgaatcgt
     3361 catgcttttc ctcttagaag aagtcgccgg cccattggca gaaaaaggcc cggcaaaaaa
     3421 tcgcgaaccc tgaacgcaaa agtgtagcag aaatagccga ttcttgattc ttctccgcgc
     3481 ttcctcagcg cagcgcacca cgcctgcccc gaccgggtcg gggcctctct gcgttaactt
     3541 tggctttttc cttcacggtg cataaggcgc acctgcaccc aatcaatctt gcaacgactc
     3601 aacgatcatc gcaatcccct gcccgcctcc gatgcacgcc gtcgccaacc cataccgcaa
     3661 tccgttccgc cgcaactcat tcaaaatcgt aatcaccagt cgcgtacccg aggccccgag
     3721 cggatgccct agcgcaatcg ccccgccgtt gacgttcgtc ttctcgcgat tcagtcccag
     3781 cactctttcc acggcaagat actgcgccgc gaacgcctca ttcacttcca cgcgatccat
     3841 ctgctccaga gtgaggcccg cgagtttcag cgctttctgc gatgacggca ccggcccgat
     3901 ccccatgatg ctcggatcga ctcctgcgac ggcccaggac acgatgcgcc ccaccggttt
     3961 caatccccgt tccttcgcga ttttctctct cgtcaccaca accgctgccg ccccatccac
     4021 gatcccgctg gcatttcccg ccgtgacgat tccatctttc ttgaaagacg gcggaagttt
     4081 ttccaaactt tccatcgtcg tttccggccg ccggtgatcg tcctcgctca ccacaatcgt
     4141 tttctttccc tgtttcactt ccactgccac cagctcttcc ttgatgcggc atgccctgta
     4201 agccgcctcc gccgcctgct ggctgcgcag cgcatacgca tcagcatcct ttcgcgtgat
     4261 gccatgctgt tcggcaagtt tttccgccgt cagtgccatc ggcaatccgc aatacgtatc
     4321 cgtcagcccc gccatcaacg aatcttccag cgctccgccg cccagcttca atcccgtccg
     4381 cgccccgcga atcacaaacg gcgcctgcgt catgttctcc atcccgcccg ccagcaccat
     4441 gttcgcttca ccaagcagca aacgctgcgc cgcttgcgcg atcgcctcga tccccgaccc
     4501 gcacagccgg ttcactgtca ccgccggcgt ttcgattttc aggcccgcct tcaatcccac
     4561 gtgccgcgcg ccataaagcg catccgcgct cgtctgcatc acattgccaa aaatggcatg
     4621 gtcaaactcg cccggatcca cacccgaacg ccgaatcgcc tctctcgaag catgcgccgc
     4681 cagctcaatc gcgctcacct ccgaaaacgc ccccgtgtac cgcgccatcg gcgtccgcgc
     4741 ccccgccaca atcaccacat ccgtaggaaa cattcttcct tctcctgttt gtctgcgctg
     4801 agttttctag ttggaacggt tgtcatcctg agcgaagcga aggatctcat tcccaactgc
     4861 cgcgcggttc cgttcggctc ttagtccaat tcttccaccg acgcgtggcc gtgtctgcac
     4921 cagtcacgag tcacgagtca caattttggc gtttccagcg ccacgacggt atccccgccg
     4981 cgctttcgtg cctcttcgat ggaaaactcc gctcggttga tcaaatccgt cacgatgtct
     5041 tcgctgtcgt actcgtgctt agccacggcc tccacaattc ccgcgctcag cgtaatttgc
     5101 gtcgagtccc acggcggccg cagtccggac gccgccctcc gcagtttatc cgcaagattc
     5161 tgcgcacccg ccagcgtcgt atccggcaaa ataaacgcca gcgaccacga agtgtatttc
     5221 accgccacat cattctgccg cacgataggc tgcaacgacc gcgccagttg ctccaaaaat
     5281 cgttccagcg gcgattctcc ctgctgccgc agcagttccg cgccgcggtc gatctgcaaa
     5341 atcgtcaacg ccagcggcgt gccctgcgtg cgcgcacggt ccgcttccgt cagcaaacaa
     5401 ttctggtacg aactgcggct cagcaaaccg gttcgctcat ccgaaacgcc catgcgccgc
     5461 accagcgatc gcagccgcgt gtggctgacg ctcatgagca tctgatcgcc aatcgcctgc
     5521 aggaaatatg cttcattcgg cttccacttg tgcgcttcgg catgaccgac aatcagcatc
     5581 cccgcgggca tctgcgtctc tttgtccgta atcatcacgc ccagcgccgt c
//
LOCUS       DASYVO010000154        22393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_32445, whole genome shotgun sequence.
ACCESSION   DASYVO010000154 DASYVO010000000
VERSION     DASYVO010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 22393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22393
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32445"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VGR03_05180"
     CDS             <1..341
                     /locus_tag="VGR03_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993703.1"
                     /translation="MLRFRPKPRGDQQSEWPIDIVGFPVTRALFSQIEGLDLLEPSSK
                     PADNVLIAQTEPGAYQNQLQDHLRRSVARVDFQHVEAPNIWLPTEDGSLLVPGPVLQA
                     VTAWISRTHP"
     gene            338..1243
                     /locus_tag="VGR03_05185"
     CDS             338..1243
                     /locus_tag="VGR03_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV1993704.1"
                     /translation="MKEEAVLFGEEKSLVGVLTNPDKEIGDSRSVGAILLNPGILHRV
                     GPGRMNVKIARALAALGLTVLRFDFSGIGDSKIRRDNLPFDKSAVDETQSAMNLLESG
                     RGIRRFILLGGCSGARVSFATACCDRRVAGVILMNFPADAEDDENLSPDLNRSRDSYY
                     FMHSALFNPGSWRKLFTGRANYRRIGGAIAFRLRRRIGRGRESSPEWDRFRGNLQLVA
                     GWGLRPVFVCSQGDIRLSDLREAGGETLKKLCSQRKADLVIIRHADHNFSSLFDQERL
                     IKITCDKVGKILQDTAKEGAPPSTN"
     gene            1766..3715
                     /locus_tag="VGR03_05190"
     CDS             1766..3715
                     /locus_tag="VGR03_05190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEV1993705.1"
                     /translation="MSNEGVSAPSRRSPREDLHLGDQKSSSGGEANRSEFGIESVAAW
                     VGNRAAATPGALAISAGSEQLSYSDLDTRANQLAHRLRAYGVGPEVVVGLFMTRSASL
                     AVGALGILKAGGAYLPLSEELPGERVDALLRDAGATVLVTELKLAHKSGGWDGQVVVL
                     DQEAEKLSHLPSSVPENSATPDNLAYVIYTSGSTGTPKGVEVSQRNLLNLIAWHQQAF
                     AVTSADRATQLAGVGFDAAVWELWPYLTAGASVHLVPDCARTAPDQLRDWLVKNNITI
                     SFVPTVLAEFLLALEWPRETSLRVLLTGADTLHRYPSPRLPFQLVNNYGPTECTVVTT
                     SGPVPATDDGGGLPPIGRPIANMRCYLLDENLRQVADGQPGELYVGGVGVARGYRNRP
                     DLTAERFIPDPFDPAKGGHLYRTGDLVRWLADGQLAFLGRTDEQIKIAGYRIEPDEIV
                     LTLEKHPDIQASCVIAREDAPDSKKLVAYLVAKPAAQLTESKLRGFLQQKLPPYMVPS
                     VFVLLDMLPITPNGKVDRAALPAPESANMLRDAAVVAPRTPIEQRLSEILSGLLGLAQ
                     VSVEDNFFLLGGHSLLGTQLIGRVRDAFGVELSLRTVFESSTIAQLSSEVEKMLLAKL
                     ESMSEEEVQRILSAPGGAPTPTSAP"
     gene            3763..4956
                     /locus_tag="VGR03_05195"
     CDS             3763..4956
                     /locus_tag="VGR03_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV1993706.1"
                     /translation="MSLYRLLDPKVLANPYPLFHRLRSEDPVHWDPYLHAWVVTRYAD
                     VQKVLLEFSAQRTPTPQQLAEMGLADLSPIADVMVRQMLFLDPPAHTRIRALASFAFT
                     PQRVEVLRSHIREITNRLIDSVASKGRMEIIADLGDPLPTTVTCEMLGVPTSDRHQLK
                     EWSQDFAEMLGNFQHNPERAPKVRRSVEEMSKYFKSAIQEIRRYPKEGLIHAFLTAEV
                     DGDRFTEEEVIANTIVTMVGGQETTTNLIGNGVMTLLRHPAEFQKLKSDLSLIPSAVE
                     EMLRFEPPSQHTARLAPHDLELGGKKIQKRQAAIAVMAAGNRDPERFPDPDRFDITRT
                     DNRHLSFGWGAHFCFGAALARIEGQSAFESLARRLPNLQLAPEPLVWRENLGLRGLTA
                     LNVTF"
     gene            5140..5838
                     /locus_tag="VGR03_05200"
     CDS             5140..5838
                     /locus_tag="VGR03_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEV1993707.1"
                     /translation="MRFASLELPFTLVTVRQDQVEGCGLHREEAVLLRGNVAQKRVAE
                     FTLGRTAARLALQELGLKNPPPVLQKAGREPSWPRGIVGSITHCGSWAMAAVARNKSL
                     QGLGIDLEDVEAVPHEEIADLVCRAAEREWVFRGGDSRLKFAMLFSAKESVYKALYPL
                     CREFFDFQAVELTWFPQRSLFRGQLCVNLNRMYRQGYAFEARCKQSANFVFTHVILGA
                     RRAATSYARDWNVT"
     gene            complement(5942..6544)
                     /locus_tag="VGR03_05205"
     CDS             complement(5942..6544)
                     /locus_tag="VGR03_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993708.1"
                     /translation="MNGTSGEMILEPSVARTLSVWWAWSWRYMLFSLAASFVLALPKL
                     GFIMLLGGTQRAAEVASQILAFATFAAGQVYTLWNLFDKRFRDFELRLEETSVGDAAP
                     ATFAYFAPTLKHATEVWWAWFWRSVLWMLGLSFGLGLLLGVLGASYLVTQANSVVLVM
                     LIWLAVSAFVLHTILGMTFSHFKLRLVPTAPSNTETFQIR"
     gene            complement(6623..6696)
                     /locus_tag="VGR03_05210"
     tRNA            complement(6623..6696)
                     /locus_tag="VGR03_05210"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6662..6664),aa:Gly,seq:ccc)
     gene            6869..8335
                     /locus_tag="VGR03_05215"
     CDS             6869..8335
                     /locus_tag="VGR03_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HEV1993709.1"
                     /translation="MAYRDLRAFIEKLEKAGELKRISVEVDPVLEIAEITDRVTRSGG
                     PALLFEKPKGSRVPLLINMLGSQRRIELALEVESVDEVAARIRGMLDFQSPQGILEKI
                     KMLPKLAEIGAFFPKTVKTGPCKQAIRKENFSLAEFPILKCWPQDGGRYITWPLVITS
                     NPETHKRNVGVYRMQVFDERSTGMHWQTQKHGAEHFRRARSRHGEGKLEVAVAIGSDP
                     VTCLSGILPIPPDMDEMMFAGFLRREPVEMVACETVDLEVPANAEIVLEGFVQLNELR
                     TEGPFGDHTGFYSLEGEYPVFHITCITQRKDPIYLTTIVGPPPQEDYYMGHAVERIFL
                     PVMKMQYPEIADISMPAEGIFHNLMIVAIRKSYPGQARKIMNAIWSLGQAMFTKVIVV
                     VDHDVNVQDYREVVWKTLCAIDPERDIQFSLGPMDTLDHAARMQDFGSKMGIDATRKW
                     ASEGFARPWPDQVVMSADVKQRIDALWSRLGLPRATKE"
     gene            8576..8788
                     /locus_tag="VGR03_05220"
     CDS             8576..8788
                     /locus_tag="VGR03_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1993710.1"
                     /translation="MVVAGMESREVMNIREASHYLGVSPDTLYRYIYEDRIPAFKLGN
                     RWKFKKTILDRWMERKMVRVRKARRA"
     gene            8818..9870
                     /gene="pilM"
                     /locus_tag="VGR03_05225"
     CDS             8818..9870
                     /gene="pilM"
                     /locus_tag="VGR03_05225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEV1993711.1"
                     /translation="MFGLGQPKALVGLDIGSSSVKAVELKASRAGYELVSFGLESLPQ
                     DTVVDGAIMDAPSVAKAIGDIFDGQKIKTKNVATSVSGHSVIVKRVTLPFMTEEELYD
                     RIQAEASQHIPFDIADVNLSYQVLEALDTQMDVLLVAVKKDKILNHTNVLAQAGKTPV
                     VVDIDAFALQNCFEVNYEPDPGQTIALLNVGAGVMSINIVRSQIPLFTRDVSVGGNQY
                     TDALQKELDLGFDDAEKLKHGESVAGVSEEQKGGILRSVSDILILEIQKTFDFFRATA
                     SGENIQRIYLAGGTARVPGLVELLREEFAMPVEELYPFRKITINPARHSEDQLREIAA
                     RLAISVGLALRSFDTP"
     gene            9867..10466
                     /locus_tag="VGR03_05230"
     CDS             9867..10466
                     /locus_tag="VGR03_05230"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEV1993712.1"
                     /translation="MIRINLLGQPRPKARRRPGPVEGGLQAVFLLVAFGLAAVALFVH
                     WGVLRSDLGGVQKEISQLQADRTRLQQVKAQVEQLQREKAARQQRVDVIKQLQRNRTG
                     GQELLDAVANTVVRVDALWMTSLVRKGNDLTMEGSADSIAAVANFITQLKRSGYFDKV
                     EISESRQDERRTNVTTFVFKLTATLTLPQDKLPSGQGKS"
     gene            10480..11124
                     /gene="pilO"
                     /locus_tag="VGR03_05235"
     CDS             10480..11124
                     /gene="pilO"
                     /locus_tag="VGR03_05235"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEV1993713.1"
                     /translation="MATSFKELPLPLQAGAYLALALVLFVAGEYVPGSPINGVRRDLE
                     QAKVQRDSLHKEVTALQVFERQGPQVKAELDAVERELEALKNIVPEDKEVDEFMRMLH
                     ESSKAANVALRRLTAKPVTPRDYHYELPFELEVDGPYYQVLDFFSRLSRLSRIINVGD
                     IEFTNAEEAKGKKYPMRPGTTVTGTCMATTFFTAGGATASANAPAKQATPPAKR"
     gene            11143..11766
                     /locus_tag="VGR03_05240"
     CDS             11143..11766
                     /locus_tag="VGR03_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993714.1"
                     /translation="MLRLPQIVRWIATLALVCVFSAASSAQTKTAPGAPAKGKAAAAK
                     PAQAKAPAAKAAPQAKAPAKKGKTAKAAAKAPAKAPAKAVESEAAAEAIPNPAAHRRD
                     PFLPLVSREQVGPAIPAHLPPGKAGLMISTLRVDGIVRSANGMLVVVTNPQQRTYFLR
                     QGDRLYDGQVEQISMEGVSFKETGKDPFGKPIERIVTKRIYPSAGEQ"
     gene            11766..14321
                     /gene="pilQ"
                     /locus_tag="VGR03_05245"
     CDS             11766..14321
                     /gene="pilQ"
                     /locus_tag="VGR03_05245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEV1993715.1"
                     /translation="MKTNGRAYRVAVLIVALLAACSLSSAWAAPGPATTLHVNAVVQP
                     TGVRIEVQASGAFEFTTYRPSENLFIADLVGVTAEGPQNARVLTSEVVSSYRVIQYRA
                     GDRPVVRLEVLLRAPVEPRVDRQGTGDLTFAFESSAPAAAAAPSAKGTPRAVETSLAT
                     KTAIHAKPIRPVHKLDAEASIAPKAKSIERLSLDAQGQQVRVRMEGDGRLAYDVTRLS
                     NPDRLVFDFSGAQLRLAKHNLAGSLEPVRGVRAAQFKPDLARVVIDLAHSTPYSVNAA
                     GNALTVTFGAPARPASAEPAVAVSTADNNVTTPTATTGAAPVAEAPAASEPAKPLVEE
                     SKASEIAPAATSETQPANPVAPAETVPATGISMPETPAQPAATLARPVLAKVEPTVPA
                     TAEPKPETSIPATPAPVAPVRPSASMQGAAADAKPAAGGKYTGEPVSVNLKDVDLKDF
                     FRLIHEISGLNVVLDPAVKGSLTIVLDDVPWDQALDIVLQNNGLDKQLDGNVLRIATN
                     DTVKKEAETKRDLVKAQAEAVAQITATRVLSYAKATSIRDTLKRFLSARGDILADDRS
                     NTLIIRDIPSVLPDIDSLIKQLDRKSQQVEIEARVVSASRSFSREIGTQFGIAGAINA
                     GRNIFGGLPGNAAFVSPLIRGIGLPPPPLVSSGSSAMPLNTNLGATTPTSGVSYAFSS
                     KNFALDFLISAAEAKGVGKLLSKPKVITQNNEKATVKQGSKIPVQTVINNTISTQFID
                     AVLKLEVTPQITADGTVYMEVMVENTAIDNGTPRVQGIPALTTQSAETKVLVNDGGTV
                     VIGGVIISNQRTDITQVPLLGSIPVIGHLFKHSTVSTDSKELLFFLTPRIVPT"
     gene            14420..15526
                     /locus_tag="VGR03_05250"
     CDS             14420..15526
                     /locus_tag="VGR03_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEV1993716.1"
                     /translation="MGTHSIRHSYLRRLELLRQRLRKAGASAILVTHPPNVYYLCGFT
                     GDSGALLVENSRLTLYTDGRFRIQAREESRGARVEIVRVSPPVAAGAALGGRRRPLEC
                     AYEDDRVTVAQLAGMRKHAGPSVRWLRLGGAVEALRVVKDAGEIRFMRDAAALASRVV
                     AEVIELIRPGMRECEVAAEIDYKLRRGGASGTSFETIVASGPRSALPHARPTGKVLAR
                     NELVVLDLGAILRGYSSDLTRTVYLGRAPKEIRRWYGAVVEAQAAAREVIRAGVAGGE
                     VDAAARGVLERAGLGRYFVHSTGHGLGLEVHENPRLAREQKNTLPAGSVVAVEPGVYV
                     PGRGGIRLEDDVLVLEKGCEVLTTASRDLIEISA"
     gene            15583..16113
                     /gene="accB"
                     /locus_tag="VGR03_05255"
     CDS             15583..16113
                     /gene="accB"
                     /locus_tag="VGR03_05255"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV1993717.1"
                     /translation="MKKAKQKSTAASGSRVDLKEMERLLAFMAHHGLEELEYQRGAVH
                     IRLRKASQGDAANPAPAVLHSMPGSAAAAEERRGGDPASKTAAAEEQLHIVKSPIVGT
                     FYEAATPGSDPFVKVGQRVKAGQVLCIIEAMKLMNEIEADRAGEVARCLVENGQPVEY
                     GQPLFALRPAPGAPGN"
     gene            16173..17522
                     /gene="accC"
                     /locus_tag="VGR03_05260"
     CDS             16173..17522
                     /gene="accC"
                     /locus_tag="VGR03_05260"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV1993718.1"
                     /translation="MFKKILIANRGEIALRIICACKELGIRTVAVYSEADRNSLHVRF
                     ADEAVCIGPPRVTDSYLNIPSVISAAEITNVDAVHPGYGFLAENANFADVCDASHLTF
                     IGPTPEVIRMMGEKDRARREMAAAGLPIVPGTPGPVADEDAAVAASREIGFPLLIKAV
                     SGGGGRGMREVHAAAELLPAFRAARHEAGQSFGAADVYIERLLVRPRHIEFQVLGDKH
                     GHVLHLGERECSIQRRHQKLIEESPSPAVDAKLRSELGAKVVKALEKINYTGAGTVEF
                     LRDESGKFYFIEMNTRIQVEHPVTEMVTDIDLVKAQIRLAAGEKLRDVVPRTDLRGHS
                     IECRINAEDPETFVPSAGHLTAFYVPGGKGVRVDTAMHSDASIPPYYDSLVAKLIVLG
                     ADRRHALLRMQRALEMFIVEGVKTTLPLHRRILADADFQAGRIDTGFLDRLLPAAGK"
     gene            18871..20112
                     /locus_tag="VGR03_05265"
     CDS             18871..20112
                     /locus_tag="VGR03_05265"
                     /inference="COORDINATES: protein motif:HMM:NF025581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HEV1993719.1"
                     /translation="MLSFSGNNHLSGFAYDASGNTLNDGTFSYGWNAESQLKSGGGVN
                     YLYDGDGRRVSKSNGKLYWYGPEASGGGDILAETNAAGTTTAEYIFFGGKRIAMLPAG
                     SNPLYYVEDLLGTSRVVTQSNGTVCYDADFYPYGGERTVTNTCPQNNYKFEGKERDTE
                     TGNDDFGARYYSNRFGRWLSADWSAVPVPVPYANLTNPQTLNLYAMVADDPESFADLD
                     GHDEGSAVHADCSAGGGNCLTQTKGSAEVATREEHSKPAPTQQNQPNNRTGYETQDQA
                     AAAALGNSNGASIKNNKEYAGLIYKDKHDGKYHFTGPDGGGNGTTSNPHNAKAPRGSK
                     VVGDYHTHGDYSLVGADGQPVRTHDSRRDDYDSNHFSPGDRSGIKSDGRGKPDYRGYL
                     GTPSGRFLVYNPSTGQEGPLQ"
     gene            21198..>22393
                     /locus_tag="VGR03_05270"
     CDS             21198..>22393
                     /locus_tag="VGR03_05270"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV1993720.1"
                     /translation="MSHPSRREFLGYTGGLGAFALMPTERVTPELILYNGNFITIDTR
                     QPRAQAVAISGGRFLAVGSNDDVRALATALTKSVDLGGKTVTPGFIDAHTHPAESGRE
                     HLREVDCDLRSISAIQTALRARAAQTPAAHWVLGFKYDDTKTAEGRPLTREDLDAVDR
                     QHPIAVTHRGGHTTYYNSMAFQLAGIDEKSPDASNGKIDRDPATRRPSGRVAESAREP
                     FYKIIPSTYTRDDRREGVKLISKMMSRTGITSVHDAFGSPNDLQAYQDARDAGELSLR
                     VYCLIGYQFIDQMIAAGVRTGLGDEWVRVGAMKAICDGSCSERTARLSAPYVGRPNDF
                     GILVMDEEEMYSHMHKAHAAGWQIGTHANGDVAIDKTLKVYERLQRESPRRDPRFRLE
                     HCTVIN"
BASE COUNT         4541 a         6862 c         6815 g         4175 t
ORIGIN      
        1 ccatgctgag gttccgtcca aaaccgcgcg gggatcaaca atccgaatgg cccatcgaca
       61 tcgtcggttt cccggtgaca cgtgctcttt tcagtcagat tgaggggctg gatctgctgg
      121 agccttcgag caagccggca gacaatgtgc tgatcgcaca gaccgaaccg ggggcctacc
      181 agaatcaatt gcaggatcat ttgaggcgaa gcgtggcccg cgtggacttc cagcacgttg
      241 aagctcccaa tatctggctt ccgaccgaag acggcagcct gctggtgccc ggcccggtct
      301 tgcaggcggt aaccgcctgg atttcccgga ctcacccatg aaagaggaag ctgttctctt
      361 cggcgaggag aagtctctgg tcggcgtgct gaccaacccc gacaaagaga tcggggattc
      421 gcggagcgtg ggtgccattc tgttgaatcc ggggatcctg caccgcgtcg gccccggacg
      481 gatgaacgta aagatcgcgc gggcgctggc tgctcttggc cttaccgtcc tccgattcga
      541 cttctcagga attggtgaca gcaagattcg ccgcgataat cttccgttcg acaagagcgc
      601 ggtggatgaa acgcaatcgg ccatgaatct tctggaatca ggacgtggaa ttcgacgctt
      661 catccttctg gggggatgct ctggcgctcg tgtttcgttc gctacggcat gttgtgaccg
      721 cagagtggct ggtgtcatcc tgatgaactt cccagccgac gcggaggacg atgagaatct
      781 gagcccggat ctgaaccgca gcagggatag ctattacttc atgcactcgg cgctcttcaa
      841 cccgggaagt tggcgtaagc ttttcaccgg cagagccaac taccggagaa ttggcggcgc
      901 gatagctttt cgactccggc gccgaatcgg tcgcgggcga gaaagctctc ccgagtggga
      961 tcggtttcga gggaatctcc agcttgtagc cgggtggggc cttcgccctg tgtttgtatg
     1021 ttcccagggc gatattcgtc tctcggatct ccgcgaggca ggaggagaaa cgctcaaaaa
     1081 attgtgttcg cagagaaaag cggacctggt gatcatccgg catgcggatc acaacttcag
     1141 ctcgcttttc gatcaggaac ggctgattaa gatcacttgc gacaaagttg gcaagatcct
     1201 tcaagacacc gcgaaggaag gcgctccacc ctcgacgaac tgaccggcac cccgcgaatg
     1261 agtccagcca ggttgcaagt tccgattcgc cttcgaggtc tccggagttt ggatttcttc
     1321 ccttcaaaag agtctgcttt cctgctcgtt tctccgcccg gctgatccga aattggtctc
     1381 ggctgtgacc gtttcaaacg acctcgagaa ctgacactct ggctggactc gcaacaggta
     1441 tgggcaaatt gccccgaaga tgagggctct cccacacaag agaatttcta caaatgtgaa
     1501 aatgttttcc atacagagct gtagttcgga gccgcgcgtt ggggtctgga ccgctgtgaa
     1561 tcatcttctt gtctaatcca cattctaaag tctttatgtc caccgatgga ttttcatagg
     1621 gttgcggcgt ggtcagtgaa ttgcacactc ctattgtgat actgagctgt gctatcgaaa
     1681 ttgaccgtgc acttgttgag aacgtgagcg cttcggcgta ctcttgcaac aggctcctgg
     1741 cacccagccc cgaggagaac cggatttgag taacgaagga gtttctgcac cttcgaggcg
     1801 cagcccaaga gaggaccttc acctcggcga ccagaaatcc tcttccgggg gcgaggcaaa
     1861 ccgctcagag tttggcattg agtctgtcgc cgcctgggtg gggaaccgcg cggccgcgac
     1921 acccggcgcc ctcgccattt ctgccgggtc cgagcaactc tcctattctg atctggacac
     1981 cagagcaaac caactggccc atcggctgcg cgcctacggc gtgggtcccg aagttgtcgt
     2041 tggcctgttc atgacgcgct cggcgtccct ggcggttggc gcacttggaa ttctcaaggc
     2101 ggggggagcg tatctcccgc tgtcggagga gctcccgggc gagcgtgtcg acgcactttt
     2161 gagggatgcg ggcgcgaccg tattggttac cgaattgaag ctcgcgcaca agagcggcgg
     2221 ttgggacggt caggtcgtgg tgctggatca ggaagcggag aaactgagcc acctgccttc
     2281 gagtgttcct gaaaattccg cgactcccga caatctcgcc tacgtgatct acacttccgg
     2341 ctccacgggg actccgaagg gcgtggaagt cagccagcgc aatttgctga atctgatcgc
     2401 gtggcatcaa caggcctttg cggtgacttc cgccgatcgt gcgacgcaac tggcgggcgt
     2461 gggattcgat gcggccgtct gggaactgtg gccttatcta accgccggcg ccagcgtcca
     2521 tttggtgccc gattgtgccc ggaccgcccc ggaccagctc cgggactggc tggtgaaaaa
     2581 caacatcacc atcagttttg tgcccaccgt attggcggaa ttcctgctgg ccctggagtg
     2641 gccgcgggag accagtctcc gcgtcctgtt gaccggcgcg gacactttgc atcgataccc
     2701 ttcgccgcgc ttgccttttc agctcgtgaa caactatggg ccaaccgaat gcacggtggt
     2761 gacgacgtcc ggaccggtcc cggcaaccga cgacggcggc gggcttcctc cgatcggacg
     2821 gccgatcgcc aatatgcgct gttatcttct cgacgaaaat cttcgacagg ttgccgatgg
     2881 gcagcctgga gagctttacg tcggcggcgt gggagtagct cgtggttacc gcaatcgccc
     2941 tgacctgacc gccgagcggt ttatcccgga tccgttcgac cccgcgaagg gcggccacct
     3001 gtaccgcacg ggcgatctgg ttcgctggct tgcggacgga caacttgcct ttctgggacg
     3061 aactgatgag cagatcaaaa tcgccggcta tcgcatcgag ccggacgaaa tcgtgctcac
     3121 cctggagaag caccccgaca tccaagccag ttgcgtgatt gcgcgcgagg acgcccccga
     3181 cagcaagaag ctcgtggcct acctcgtcgc caagcccgca gcgcaactga ccgagagcaa
     3241 gttgcgcggc tttctgcagc agaagttgcc cccgtacatg gtcccctcgg tcttcgtgct
     3301 gctcgacatg ttgcccatca cgcccaatgg caaggtggac cgggcggcgt taccggcccc
     3361 ggaatcggcg aatatgctgc gggatgcagc cgtggtggct ccgcgcactc ccatcgagca
     3421 gcgtctgtcg gaaatcctct cggggcttct cggcctggca caggtgagcg tggaagacaa
     3481 ttttttcctg ctgggtggcc actccctcct gggcactcaa ttgattggca gggtccgcga
     3541 cgcattcggc gtggagctgt cgttgcggac cgtgtttgaa tcctccacca tcgctcagct
     3601 ttcctccgaa gtcgaaaaaa tgcttctcgc caagttggag tccatgagcg aggaagaggt
     3661 ccagcgaatc ctcagcgcgc cgggcggggc cccgacgccg acgagcgcac cttgaacggc
     3721 cggcacccga ctgctcctcc ctcggaaaag cggccgcccg ttttgagcct gtaccgcctc
     3781 cttgacccta aggttcttgc caatccctat ccgctctttc accgcctgcg ctcagaggat
     3841 cccgtccatt gggaccccta tctccacgcc tgggtggtga cccgctacgc ggacgtccag
     3901 aaggtcttgc tcgagttttc cgcccagcgc acgccgacgc cgcaacagct tgccgagatg
     3961 gggctggccg atctatcgcc catcgccgac gtcatggtcc ggcaaatgct cttcctggat
     4021 ccgcctgccc ataccagaat tcgcgccttg gcgtcatttg cgtttacgcc ccagcgggtg
     4081 gaggtcctgc gttcgcacat ccgggagatc acaaatcgat tgatcgattc ggtggcatcg
     4141 aaaggccgga tggagatcat cgccgatctc ggggaccccc tgccgaccac ggtcacctgt
     4201 gagatgctcg gtgtgcccac ctcggatcgt catcaactca aggagtggtc ccaggatttc
     4261 gccgagatgc tcggtaactt tcagcacaat cccgagcgcg cgccgaaagt gcgccgaagc
     4321 gtcgaggaga tgagcaagta tttcaaatcc gccatccaag agattcgcag atatccgaag
     4381 gaaggtttga ttcatgcctt cctgaccgcc gaggtggatg gcgatcgatt caccgaggaa
     4441 gaagtgatcg ccaacaccat cgtcaccatg gttggcgggc aggagacgac caccaacctc
     4501 atcggaaacg gagtgatgac cctgcttcgc caccccgcag aattccaaaa gctgaagtcc
     4561 gatctctcct tgattccttc tgcggtcgaa gaaatgctcc ggttcgaacc cccgagccag
     4621 cacaccgctc gcctggcccc tcacgacctg gaattgggcg gtaagaaaat tcaaaaacgt
     4681 caggccgcca ttgccgtgat ggcggcaggc aatcgcgacc cggagcgatt ccccgacccc
     4741 gaccggttcg acatcacgcg caccgataac cgccacctgt cattcggctg gggcgcgcat
     4801 ttctgtttcg gggccgctct cgcgcgaatc gaggggcagt ctgccttcga atcgctggcg
     4861 cgaaggcttc ccaatcttca gctcgcgccc gaaccgcttg tctggcggga aaacctgggt
     4921 ttgcgcggac tgacggcact caacgtgacg ttctgaggcg gctgcttccg gcacaagggt
     4981 cgctgtgaaa cctggcgggc aaagctgatg tcagcggtgt tggaaaagat gaaacggccg
     5041 ttcccttcgc ccgtcgaggc gagtgaaatt ccctctgccg tgccaacccg ggttcaacct
     5101 gcgctttcag cgcgtgcatc ggtggagtgc ggaccccaat tgagattcgc atctctggag
     5161 ctgccgttca cactcgttac ggtccggcaa gatcaagtcg aaggatgcgg gttgcaccgc
     5221 gaagaagccg tcctgttgcg gggaaatgtc gcgcaaaaac gcgtggctga atttacactg
     5281 gggcgcaccg ccgcgcgcct cgccctgcaa gaactgggtc ttaagaatcc accacccgta
     5341 ttgcagaagg cgggccgcga accgtcgtgg ccgcgaggga tcgtcggctc tatcacccac
     5401 tgcggctcct gggccatggc tgcggttgcc aggaacaaat ctttgcaagg actcggaatc
     5461 gacctcgagg atgtggaggc cgttcctcat gaagaaattg cggatctggt gtgccgcgcg
     5521 gcggaacgtg aatgggtgtt ccggggcggg gacagccggc tcaagttcgc catgctcttt
     5581 tccgccaagg aatcggtcta caaggcgctc tatcctctct gccgcgaatt ttttgatttc
     5641 caggccgtgg agctcacctg gttccctcaa cgcagccttt ttcggggcca gctctgcgtg
     5701 aatctgaacc gcatgtatcg ccagggctac gccttcgaag cccgctgcaa gcaaagtgca
     5761 aatttcgtgt ttacgcatgt aatccttgga gcccgccggg cggcgacctc ctacgcccgc
     5821 gattggaatg tgacctaggc cgtaccgttt gcggtcaaat ctcttagtcc acatttctca
     5881 ccacaaatca aaggaccagg cagaccagaa tcacccttgg ttgtcgggcc gtgcgagcgg
     5941 attagcgaat ttggaaggtt tcagtgttgc tcggcgcggt aggaacaagg cgcaatttga
     6001 aatgcgaaaa cgtcattccc agaattgtgt ggagaacgaa ggcggacacg gcaagccata
     6061 taagcatgac cagaacgacg gagttggctt gggtaacgag ataggaagcg cctaacacac
     6121 cgaggagcaa gcctaggccg aaggagagtc caagcatcca aaggacggac ctccaaaacc
     6181 aggcccacca cacctccgtg gcgtgcttga gcgtcggcgc aaagtaggca aacgtcgcgg
     6241 gcgccgcatc gccgactgaa gtttcctcca gcctcagctc gaaatctcta aagcgcttat
     6301 cgaagagatt ccagagcgtg tacacctggc ctgcagcgaa agtggcgaat gctagaattt
     6361 gagaagccac ctcagcagca cgctgcgtgc cgccgagcag catgataaag cccagcttag
     6421 gaagggcgag cacgaacgat gcggccaggc tgaacagcat gtaccgccag gaccacgccc
     6481 accatacgga gagcgtgcgc gcaactgatg gctcgagaat catctccccg gaggttccgt
     6541 tcatcttcgc tctacagagt gcgcgcttca acagcgctta cgctatacca acgacttttc
     6601 cgaggttttt gtttgggtct tctggagcgg gcgatgggaa tcgaacccac gtccgttgct
     6661 tgggaagcaa caattctgcc attgaaccac gcccgctctg ttgccacctt agcaaatctc
     6721 aaagcgaatg ggtagtgctt gcagcaaagg cacctgcccg gccggaaggc tccgaactgt
     6781 ctgcatgcga accgaaaaac agcacagcca ggagtggctg tgctactgag attccgtgct
     6841 agattgttgc ctcatttgag gtgccgaagt ggcctatcgc gacctgcgcg cgttcatcga
     6901 aaaattggaa aaggcaggtg agctgaagcg catcagcgtc gaggtggatc cggtcctcga
     6961 gattgccgag atcaccgacc gcgtcacgcg ctcgggcggc cccgctctgt tgtttgagaa
     7021 gcccaaaggc tcgcgggtgc ctctactcat caacatgctg ggcagccagc ggcgcatcga
     7081 gctggcgctc gaagtggaat cggtggacga agttgccgcg cgcattcgcg ggatgctcga
     7141 ctttcagtcc ccgcagggca tcctcgaaaa aataaagatg ctgccaaagc tggcggagat
     7201 cggcgcgttt tttcccaaga ccgtcaagac cgggccgtgc aagcaggcga tccgcaagga
     7261 aaacttctcg ctcgctgagt ttccaattct caaatgctgg ccgcaggacg gcggccggta
     7321 catcacctgg ccgctggtga tcaccagcaa tcccgagacg cacaagcgca acgtcggcgt
     7381 ctaccgcatg caggtgttcg atgagcgctc gacggggatg cactggcaga cgcagaagca
     7441 tggcgccgag catttccgcc gcgcgcgctc gcgccacggg gaagggaagc tcgaggtggc
     7501 cgtggccatc ggcagcgatc cggtcacctg cctttccggc atcctgccca tcccgccgga
     7561 catggatgaa atgatgttcg ccggtttctt gcggcgcgag ccggtggaga tggtggcctg
     7621 cgagacggtg gacctcgaag tccccgccaa cgccgagatt gtgctcgagg gcttcgtcca
     7681 gctcaacgag ctgcgaaccg aaggcccctt cggagaccat accggcttct attctttgga
     7741 aggcgaatac ccggtcttcc acatcacctg catcacccag cgcaaagatc ccatctatct
     7801 gaccaccatc gtcgggcctc cgccgcagga ggactactac atgggccacg ccgtggagcg
     7861 aattttcctg ccggtgatga agatgcagta tccggagatt gctgatatct ccatgcccgc
     7921 ggaaggaatt tttcacaacc tgatgattgt ggccattcgt aagtcctacc ccggccaggc
     7981 gcgaaaaata atgaacgcga tctggtcgct ggggcaggcc atgttcacca aggtcatcgt
     8041 cgtcgtcgat cacgacgtga acgtgcagga ctatcgcgaa gtggtgtgga agacgctgtg
     8101 cgccatcgat cccgagcgcg acattcaatt ctcgctcggc cccatggaca cgctggacca
     8161 cgccgcgcgc atgcaggact tcggatccaa gatgggaatc gacgccacgc gcaagtgggc
     8221 cagcgaagga ttcgcgcgtc cctggcccga ccaggtggtg atgagcgcgg acgtaaaaca
     8281 gcgcatcgat gcgttgtgga gccgtctggg cctgccgaga gcgaccaaag agtaagcctc
     8341 ggtgtgttct ggtcgatata cccagccctt ggggtgcgag ggggacagcc tactatatat
     8401 tgggactacc ggggctttct gcggtcaata ccccaagaaa taccaaggcg ggctgcccgc
     8461 gcaccgaatc ccccgcttga cactctgccc ggcgggtgac ataacgcgcc aagcaagtct
     8521 ctgccaggtg gatgtctgcg gtggtcgggg ccctgtgcct ggagggaccg ggtagatggt
     8581 ggtggcggga atggaatcgc gcgaagtgat gaacatccgg gaagcgtccc actacttggg
     8641 cgtttctccg gacaccctct accgctatat ttatgaagat cggattcctg ctttcaagtt
     8701 ggggaaccgc tggaaattta aaaaaactat tctggaccgt tggatggagc gaaagatggt
     8761 ccgggtaagg aaagccaggc gagcctgaaa gtcgaggcgg catagagggg cgggacgatg
     8821 ttcggcttag gacaaccaaa agcgctggtg ggactggata tcggttcgag ctcggtgaag
     8881 gctgtggagc tcaaggcttc gcgcgccggc tatgagctgg tcagctttgg cctcgaatcg
     8941 ctgccgcaag acaccgtggt cgacggcgcc atcatggatg ccccgtcggt ggccaaggcc
     9001 atcggcgaca tcttcgacgg ccagaagatc aagaccaaga acgtggccac ctcggtttcc
     9061 ggccactcgg tgattgtgaa gcgggtcacc ctgccgttca tgaccgaaga agagctctac
     9121 gaccgcatcc aggccgaagc cagccagcac atccccttcg atatcgcgga cgtgaacctc
     9181 agctaccagg tgctcgaggc gctcgacacg caaatggacg tgcttctggt ggcggtcaag
     9241 aaagacaaaa ttctcaacca caccaacgtg ctggcccagg ccggcaagac accggtggtg
     9301 gtggacatcg acgcgttcgc tttgcagaat tgcttcgaag tgaattatga gccggatccc
     9361 ggccagacca tcgcactcct caacgtgggc gccggcgtga tgagcatcaa catcgtacgc
     9421 agccagattc ccttgttcac ccgcgatgtg tccgtcggcg gcaaccagta cacggacgcc
     9481 ttgcagaagg agctcgacct cggcttcgat gatgcggaaa agctcaagca tggcgaaagc
     9541 gtggccgggg tcagcgagga gcagaagggc ggcattctgc gctccgtctc cgacattctg
     9601 attctggaaa ttcagaaaac ttttgacttc ttccgggcca ccgcaagtgg cgagaatatc
     9661 cagaggatct acctcgcagg aggaacggcc cgggtgcccg gcctggtcga gctgctgcgg
     9721 gaagagttcg ccatgccggt ggaggagctg tacccgtttc ggaagatcac catcaacccg
     9781 gcccggcaca gcgaagacca gctacgggag attgcggcgc gcctggcgat ttcggtcgga
     9841 ctcgccctga ggagctttga cacgccatga tccgaattaa ccttctcggc caaccgcggc
     9901 ccaaggcccg gcggcgtccc ggcccggtgg aaggcgggct gcaagcggtt ttcctgctcg
     9961 tcgcctttgg gctggcggcc gtcgcgctgt tcgtgcactg gggtgttctg cgcagcgact
    10021 tgggcggcgt acaaaaagag atcagccagt tgcaagccga tcgcacacgc ctgcaacagg
    10081 tgaaggccca ggtcgagcag ctccagcgcg agaaagccgc gcgccagcag cgcgtcgacg
    10141 taatcaagca gttgcagcgc aatcgcaccg gcggccagga gcttctcgac gccgtggcca
    10201 acaccgtcgt gcgcgtcgat gccttgtgga tgacctcgct ggtgcgcaaa gggaacgatc
    10261 tgaccatgga aggatcggcg gattccatcg ccgcggtggc caacttcatc acccagctca
    10321 agcgttccgg gtatttcgat aaggtcgaaa tcagcgagtc caggcaggat gaaagaagga
    10381 cgaacgtgac cacgtttgtc ttcaaactga cggccacttt aacgctgccg caggataagt
    10441 tgccctcggg gcagggcaag agctgaggga gagagcgtca tggcgacttc tttcaaagaa
    10501 cttccgctgc cgttgcaggc gggggcctat ctggcgcttg cgctggtgct cttcgtggcg
    10561 ggcgagtacg tgcccggctc gccgatcaac ggtgtgcggc gcgacctcga gcaggccaag
    10621 gtgcagcgtg acagtttgca caaggaagtg accgccctgc aggtgtttga gcggcagggc
    10681 ccgcaagtga aggccgaatt ggatgcggtc gaacgcgagc ttgaggcgct gaagaatatc
    10741 gttcccgagg acaaggaagt cgatgagttc atgcgcatgc tgcatgaatc ttccaaggcc
    10801 gccaacgtgg cgctgcggcg gctgaccgcc aagcccgtta ctccgcgcga ctatcactat
    10861 gagctgccct tcgagctgga agtcgacggc ccgtattacc aggtgctgga cttcttttcc
    10921 cgcctgagcc gtctctcccg gattatcaat gtcggggaca tcgagttcac caacgcagag
    10981 gaagccaagg ggaagaagta tccgatgagg ccgggcacga cggtgaccgg aacatgtatg
    11041 gccacgacgt tttttaccgc cggcggagca accgcttcgg cgaacgcacc ggccaagcag
    11101 gctacgccgc ctgcgaagcg ctaggcgatg gaaggtttga ccatgcttcg cttacctcaa
    11161 atcgtgagat ggattgccac tctcgccctg gtgtgcgtat tctccgcagc ctcaagcgct
    11221 cagaccaaga cggcgcccgg cgcgccagcg aaaggcaagg ccgcggccgc aaaaccggcg
    11281 caggccaagg cccccgctgc caaggcggcg ccgcaagcca aagcccccgc caagaagggc
    11341 aagacggcaa aggccgcggc gaaggcgccg gcgaaggcgc ccgcgaaggc cgtggagagc
    11401 gaagcagccg ccgaggccat tcccaatccc gccgcccacc ggcgcgatcc tttcttgccg
    11461 ttggtgagca gggaacaggt tggtccggcc attcccgcgc atctaccgcc cgggaaggca
    11521 ggcttgatga tctcgacgtt gcgcgtggac ggcatcgttc gcagcgccaa cgggatgctg
    11581 gtggtggtca ccaatcctca gcaacgcacc tattttctac gccagggtga ccggctctac
    11641 gacggccaag tggaacagat cagcatggaa ggcgtgtcat tcaaagaaac gggaaaagat
    11701 ccgttcggca agcccattga gcggattgtg accaaacgaa tttacccgag tgcaggagaa
    11761 cagtaatgaa gacgaacggg cgagcctatc gggtcgcagt gctaatcgtg gcgctccttg
    11821 cagcgtgttc cctgagttcg gcctgggctg cgcctggtcc ggccaccacg ctccatgtca
    11881 acgccgtggt tcaacccacc ggcgtgcgga tcgaggtgca agcctcgggc gcctttgagt
    11941 tcactaccta tcgtcccagc gagaacctgt tcatcgccga cttggtgggg gtgaccgccg
    12001 agggcccgca gaatgcccgc gtgctgacct ccgaagtggt gagcagctat cgcgtgatcc
    12061 agtatcgcgc cggggatcgt cccgtggtgc ggctggaagt tctgctgcgc gctccggtcg
    12121 agccgcgcgt cgaccgccag ggaaccggcg atctgacatt cgcgttcgag agcagcgctc
    12181 ctgctgcggc cgctgcgccg tccgctaagg gtacaccgcg ggccgtggaa acttctctgg
    12241 cgacaaagac cgcaattcat gccaaaccca tccggcccgt ccacaagttg gatgccgagg
    12301 cgtccatcgc tccaaaggcg aagtccattg agaggctgtc gcttgacgcg cagggccagc
    12361 aggtgcgggt tcgcatggaa ggcgatggcc gtctggcgta cgacgtaacc cggctgagca
    12421 accctgaccg tctggttttc gacttttccg gggcgcagct ccgcctggcc aagcacaatc
    12481 ttgccggttc tctggagccg gtgcgcggtg tgcgcgccgc tcagttcaag ccggatctgg
    12541 cgcgcgtggt catcgatttg gcgcacagca ctccctatag cgtgaatgcg gcgggaaatg
    12601 cgctcaccgt gacgtttggc gctcctgcgc gcccggcgtc tgcggaacct gccgttgcgg
    12661 tctctaccgc ggacaacaat gtaaccactc ccactgccac gacaggcgcg gctcccgtcg
    12721 cagaggcacc cgcggccagc gagccggcca agcccctggt agaggaatca aaggcctccg
    12781 agattgcacc ggccgcgacg agcgaaactc agccggcaaa cccggtggcg ccggccgaaa
    12841 ccgtaccggc aacagggatc tcgatgccgg aaactcctgc gcagccggcc gcgacactgg
    12901 cccggcccgt tctcgcaaag gtggaaccga ccgttcccgc cactgcggaa ccaaagcctg
    12961 aaacatcgat cccggccacg ccggcccccg tcgctccggt gcggcccagc gcttccatgc
    13021 agggcgctgc agccgatgcc aagccggccg ccggcgggaa gtacaccggt gaaccggttt
    13081 cggtcaactt gaaggacgtg gatctgaagg atttcttccg gctgattcac gagataagcg
    13141 gtctgaacgt cgtgctcgat cctgcggtga agggctctct gaccatcgtg ctcgacgatg
    13201 tgccgtggga tcaggccctg gacatcgtgc tgcagaacaa cggcctcgac aagcagctcg
    13261 acggaaacgt tctgcgcatc gccaccaacg atacggtgaa gaaggaagcc gaaaccaagc
    13321 gcgacctggt caaggcgcag gcggaagccg tcgcccagat aaccgcgacg cgcgtgctca
    13381 gctacgccaa ggccacctcg attcgcgata ccctgaagcg tttcctttcc gcgcgcggtg
    13441 acattctggc ggatgaccgt tccaataccc tgatcatccg cgacatcccc tcggttctgc
    13501 ccgatatcga ctcgctgatc aagcaactgg accgcaagtc ccagcaggtc gaaatcgaag
    13561 ccagagtggt ctcggcttcg cgttcgttct cccgcgaaat cggcacccag ttcggaatcg
    13621 cgggcgcaat aaacgccggc cggaacatct ttggcggtct gccggggaat gcggcattcg
    13681 tcagcccgct gatccgagga attgggctgc ctcccccgcc gcttgtttca tcgggcagct
    13741 cggcgatgcc cctgaatacc aatctcggcg ccactacgcc tactagcgga gtttcctacg
    13801 ccttcagctc aaagaacttc gcgctcgatt tcctcatttc cgccgcggag gccaagggcg
    13861 tcggcaagct gctctccaag ccgaaggtga tcacccagaa caacgagaag gccacggtca
    13921 agcagggctc gaagattccc gtgcagaccg tcatcaataa cacgatttcc acgcagttca
    13981 tcgacgccgt gctcaaactc gaagtgaccc cgcagatcac tgcggacggc acggtgtata
    14041 tggaagtcat ggtagagaac accgccatcg acaacggcac accgcgcgta caaggcatcc
    14101 cggctcttac cacgcagtcg gcggagacca aggtgctcgt caacgacggc ggcacggtgg
    14161 tcatcggcgg ggtgatcatc tccaaccagc gcaccgacat cacccaggtg ccgctgctcg
    14221 gaagcattcc cgtgatcggc catctgttca agcactccac ggtgagcacg gactcgaagg
    14281 agctgctctt cttcctgacc ccgcgaatcg ttcccaccta gtctgcggaa cattcgttag
    14341 acggggcgac acaagcaccg gtagaatcga ggaggaggca agggcctcct cctctctttt
    14401 tatgcctgct catcttttta tgggtactca ttcgattcgc cattcctacc tgagacgcct
    14461 ggagcttctg cggcagcggc tgcggaaggc tggggcgtcg gcgatcctgg tgactcaccc
    14521 cccaaacgtc tattacctct gcggtttcac gggcgattcc ggcgcactgc ttgtcgagaa
    14581 ttcccgattg acgctctaca cggatgggag attccgcatc caggcgcggg aggagagccg
    14641 gggcgcccgc gttgagatcg tgcgggtgtc accccccgtc gccgcgggag ccgccctggg
    14701 aggccggcga aggcccctgg agtgtgccta cgaggacgac cgggtcactg tcgctcagct
    14761 tgcggggatg cgcaagcatg ccggcccctc cgtccggtgg ctgaggctgg ggggcgcggt
    14821 ggaggcccta cgcgtggtga aggatgcggg ggagattcgg ttcatgcgcg acgcggcggc
    14881 actggccagc cgtgtggtag ccgaggtgat cgagttgatc cgtcccggca tgcgcgaatg
    14941 cgaagtcgcc gcggagatcg actacaaact gcggcgtggc ggagccagcg ggacatcctt
    15001 tgaaaccatc gtcgcctcgg gtccccgctc ggcgctgccc catgcacgtc cgaccggcaa
    15061 ggtgctggcc agaaatgaat tggtcgtcct ggacctgggt gctatactgc gcggctattc
    15121 gagcgacctc acccgcaccg tctatctggg ccgcgctccc aaggagatcc gccgctggta
    15181 tggcgccgtc gtcgaggcgc aggcggctgc gcgtgaggtg attcgcgccg gggtcgccgg
    15241 cggtgaggtg gacgcggcgg cccggggcgt actcgagcgg gccgggttgg gccgttattt
    15301 cgtgcacagc accggccacg ggctgggtct cgaagtccac gagaacccgc ggcttgcccg
    15361 ggagcagaaa aacacgttgc cggcgggcag cgtggtggcc gtcgagccgg gggtatacgt
    15421 ccccggccgc ggcggcatcc gcctggaaga cgacgtcctg gtccttgaga aaggctgtga
    15481 ggtactgacc acggcgtccc gagacttaat tgagatttca gcttgaacct cgggccccgg
    15541 acttgagccc agtcctgggg ccagacctaa gagcgaccga gaatgaagaa agcaaagcaa
    15601 aaatcgacgg ccgcatccgg ctcccgcgtt gacctcaagg agatggagcg gctcctggct
    15661 tttatggcgc accatgggct ggaggagctg gagtatcagc gtggcgccgt gcatatccgc
    15721 ctgcggaagg cctctcaggg cgatgcagcc aacccagcgc cggccgtact gcattccatg
    15781 cctggttctg ccgcggcggc ggaagaacgg cgtgggggtg atccggcgtc gaagaccgcg
    15841 gctgctgagg aacagctcca catcgtgaag tcgcccatcg tgggcacctt ctacgaagcg
    15901 gcgacgcctg gctcggaccc cttcgtcaaa gtcggccagc gggtgaaggc cggccaggtg
    15961 ctctgcatca tcgaggccat gaaactgatg aacgagattg aagccgaccg tgccggcgag
    16021 gtggcccgct gcctggtcga aaacggccag cccgtggaat acggtcagcc cttgttcgct
    16081 ttgcgtcccg ccccgggggc cccggggaat tgacaccgcg gccccactcc ataggctgtc
    16141 atgcatagag agcttccgca tctttagaag acatgttcaa gaaaatcctc atcgccaatc
    16201 gcggagaaat cgcgctgcgc atcatctgcg cgtgcaagga gctgggcatc cgtacggtgg
    16261 ccgtttactc cgaggccgac cgcaattcgc tgcacgtgcg ctttgccgat gaggccgtgt
    16321 gcatcggccc gccgcgggtg accgacagtt acctgaacat tcccagcgtc atcagcgcgg
    16381 cggagatcac caatgtcgac gccgtccacc ccggctacgg cttcctggcg gaaaacgcca
    16441 acttcgccga tgtctgcgac gcttcccatc tcacctttat cggtcccacg ccggaggtga
    16501 tccgcatgat gggagagaaa gaccgcgcgc ggcgggagat ggccgcggcg ggactcccga
    16561 tcgtgccggg cactccgggg ccggtggcgg atgaggacgc cgccgttgcc gcctcgcgcg
    16621 agattggctt cccgctcctc atcaaggcgg tgtccggcgg cggcggccgg ggaatgcgcg
    16681 aggttcacgc cgccgcggaa ttgctgcccg ctttccgcgc cgcccgccac gaggcgggcc
    16741 agagcttcgg tgcggccgat gtctacatcg agcgattgtt ggtgcgcccg cggcacattg
    16801 aatttcaagt gctgggtgac aaacacggcc acgtgctcca tctcggcgag cgcgagtgca
    16861 gcatccagcg gcggcatcag aagctgatcg aggagtcgcc gtctcccgcc gtggatgcca
    16921 aactgcgcag cgaactgggc gccaaggtgg tcaaggcgct cgagaagatc aactacaccg
    16981 gcgcgggcac cgtggaattt ttgcgcgacg agtccggcaa attctatttc attgagatga
    17041 acacgcgcat tcaggttgag catcccgtca cggagatggt caccgacatc gacctggtca
    17101 aggcgcagat tcgcctggcg gcgggcgaaa agctccgcga cgtggtgccg cggaccgacc
    17161 tgcgcggcca cagcatcgag tgccgcatca acgccgagga cccggaaacc tttgtgccct
    17221 cggcgggcca cctcaccgct ttttatgtgc ccgggggcaa gggcgtgcgc gtggatacgg
    17281 ccatgcacag cgatgccagc atcccgccct actatgactc gctggtggcc aagctgatcg
    17341 tgctcggcgc ggaccgccgc cacgccctcc tgcggatgca gcgcgccctg gagatgttta
    17401 tcgtcgaggg cgtgaagacc acgcttccgc tgcatcgccg gattctggcc gacgcggatt
    17461 tccaggcggg acggattgat acggggttcc tggaccggct gctgccagcg gcggggaagt
    17521 aggctggcgt ttcgtgagct gtagatcccg gattggagaa ggccgttaac ctttaagtta
    17581 ccgccccccc tccccccttc tcttgtaagt atgcattcca aaggagttgc agggcccact
    17641 ctcgtaagta tgcattctaa aggagatgga gagcccgctc agtggacagt gatcccgaag
    17701 cttcgggagt ggtcagctta actttgcgat aatcgccaca acgtttcgta ccggaaatgg
    17761 tcaagtggtt gaacttacgg gcgttagcag aattctgatc gcaacaaaat ggggtagtcg
    17821 agatgaccgc acccttcggc ttgctcaggg tcagcatcct ggtcggatgt tgagaatggc
    17881 gcggacaagg tttcaggggt tgggatcggg aagattccgg gcgtcccgcg aggggtgaca
    17941 aattttggtt tcccagacag cgcaaactgg gcccgcctcc aaaacttatt aattatatca
    18001 cttctaaggc aatactgcta atgataaaga gatgcttcgg aatgtcgctg cgccgccaac
    18061 tttggggcgc tccgcgacgg ccttcggcag agaggaagga aacaggtcgt ttgtagaccc
    18121 ccggaccagg tcgagagaga cggggaggac gttttggaac tcgtgggccg tgagcgctgg
    18181 cgggggaggt cctttcgggg agttgcgttt caattcttcg gggatcgggt cttttggcat
    18241 cgggcgttgg gggattagga gcgggaccga aggccccacc ctttgctgaa cggcgcaaag
    18301 gatggggcac ccccttggat taaagtgtgg gccacccgcc ggataggggg taggggatag
    18361 cgggcagaga gtggaaagag gagaccgaag gccccaccct ttccggcacg aaccgccgga
    18421 aaggatgggc cacccgccag aaaacgcgct agtatcgaga gacaccctct caggtcaaag
    18481 cccgggccac ccgcctggag tgagaaacaa tatgggatta tcggtcgcaa gcctaggatt
    18541 ttggaagcta actttactcg gattcgggat ggagctattg catgcctggc tgcaactgcg
    18601 ttggccggtg atccggggct ctcgcttagg gactcggcca ctagcagcgg taatcttcat
    18661 gacctgtgag gcagtcatag tcttagctgc cgtgagtgcc ggcgtggctc ttatggcaaa
    18721 cgcttttgaa tcgcacactc tgaatgatcc gaagctgctg ctcaaagcag cgggaatgtt
    18781 agctctcaca ggttgcttgt tctggctgtg gtccttgtaa atgtgtgttc cgctcgcttt
    18841 actgatccgc tccaggacgg gccgtactcg atgctgagct tcagcggcaa caatcacctc
    18901 tccggctttg cctacgatgc ttccggcaac acgctgaacg acggcacctt ttcctatggc
    18961 tggaacgcag aaagccagct aaaatccggc ggtggcgtca actatctcta tgacggcgac
    19021 ggccgccgcg tctcgaaatc gaacggcaag ctctactggt acggtcccga agcttcggga
    19081 ggtggcgaca tcctggccga aaccaacgcc gccggcacca ccaccgccga atacatcttc
    19141 ttcggcggca agcgcatcgc catgctcccc gccggcagca acccgctcta ttacgtcgaa
    19201 gacctgctgg gcacctcccg cgtggtcacg caatcgaacg gcacggtctg ctacgacgcc
    19261 gatttctatc cctacggcgg cgagcgcacg gtcaccaaca cctgcccgca aaacaactac
    19321 aagttcgaag gcaaggaacg cgacaccgaa accggcaacg acgatttcgg cgcgcgctac
    19381 tactccaacc gcttcgggcg ctggctcagc gccgactggt cggcagtccc cgttcctgtc
    19441 ccctacgcca atctcaccaa tccgcaaacc ctcaacctct acgccatggt cgcggatgat
    19501 ccagaatcct tcgccgacct cgacgggcac gacgagggct ccgcggttca cgcggactgt
    19561 tctgccggag gtggtaactg cctaacgcaa acaaaggggt ccgccgaggt agcaaccagg
    19621 gaggagcact cgaaacccgc gccgacgcag caaaatcagc cgaacaaccg tactggatat
    19681 gagacgcagg atcaggcggc cgccgcagca ctcgggaact caaatggcgc atcgataaaa
    19741 aacaacaaag aatacgccgg gcttatctat aaggacaagc acgacggtaa gtatcatttt
    19801 acgggaccgg atggcggcgg caatggcaca acctcaaacc cccacaacgc aaaggccccg
    19861 cgaggatcaa aagttgtcgg cgactatcac acgcatggag actactctct cgtgggagcg
    19921 gatggacaac cagtccgaac acacgactct cgcagagacg attatgatag caaccatttt
    19981 agccccggtg acaggtccgg gattaagagc gacgggagag gaaagcccga ttatcggggc
    20041 tatctcggta cgcccagcgg cagattctta gtttacaatc cctcaacggg acaggaaggg
    20101 ccacttcaat gaagagatat atgtgggcta tcgtcgcaat agtgttgatt tgtggaggaa
    20161 gtatgtcaat gggccaatcg tacccgctta aaaaaatagc aaagttcccc ggcgcttctc
    20221 tgaaatggat acatcttgcg gaacccgagt ttgagcgaca taagcttgat ttggacaagt
    20281 ataacgtcga agtcatcgaa gaaattgatt ctataactgt attcttaagc tcaaccgatg
    20341 ccccagaagg atcaagggga agcgggggaa aatacccagg gtaccaagta gaatttagca
    20401 agaaggatat gaagatcttg cactcaaact acactcgatg acgacgcaga gttctcggca
    20461 gtggagaagc tcagccgcaa gagcagaacg gacctagcgc aacctgcgtg ggcgagcacg
    20521 agtggggtgc cgcatccttt ccgttcgcgg aacggattcg gggtttgact ttgattcctg
    20581 cgtctcgtca gagcaacctc cactcgcctg ccgtgatccc gcggcttcgg gacgaagggc
    20641 cgctgatcgc tgaacattga ctaccactga ccacttcccg ctcgccttgc ccgtgtctac
    20701 aaacgaccgg ttcccttcct ttctgccgaa ggccttcgcg gaggcgcgca ctcccgcgcc
    20761 cgcagcgaca tgccgcagtc gcgggaccaa gggccaattc ccgtccccct ccccggcccg
    20821 cagtcaagaa acggacgact ggtttgcttc ctctctgccg aaggctgtcg cggaggcgcg
    20881 cactcccgcg tccgcagcga cattccggat cgtttctccc acgcgggtgg tccggaagcc
    20941 aagtgaagag atcaactggg gcgtccctcg tcccttctcc gcgcggtgaa ttgcagaagt
    21001 ggtaccctct catcgaagcc tgtcgacatg acgcaagcag gagaaaaaga cccgggccgc
    21061 ccgcccaggc cttctccacc ctccaacctc cgacttccaa cttccaactt tcagacctct
    21121 ggacgcccgt ctttgcccgg catacaatcc ctgccaccag gacagtgccc tttggagtga
    21181 ccctttggag taaatcgatg agccatccct cacgccgcga attcctcggt tacacaggag
    21241 gcctgggcgc cttcgccctg atgcccaccg agcgcgtcac tcccgagctg attctctaca
    21301 acggcaactt catcaccatc gatacccgcc agccccgggc gcaggcggtc gcaatttccg
    21361 ggggccgctt cctcgccgtg ggcagcaacg acgacgtgcg cgcgctggcc accgcgctca
    21421 ccaagagcgt tgatctcggc ggcaagacgg tcactcccgg cttcatcgac gcgcacacgc
    21481 atcccgccga atcgggccgc gagcatctcc gcgaagtgga ctgcgacctg cgctccatct
    21541 cggccattca gaccgcgctt cgtgcccgcg ccgcgcagac ccccgcagcc cactgggtgc
    21601 tcggcttcaa gtatgacgat accaaaaccg ccgaaggccg cccgctcacg cgcgaagatc
    21661 tcgacgccgt ggacaggcag catcccatcg ccgtcaccca ccgcggcggc cacacgacgt
    21721 attacaactc gatggcgttc cagctcgccg gcatcgacga aaaatctccc gacgcttcga
    21781 acggcaagat cgaccgcgat cccgcaacgc ggcgaccgtc cggccgcgtg gccgaaagcg
    21841 cgcgcgagcc gttctacaaa atcatcccgt ccacctatac gcgcgacgac cgccgcgagg
    21901 gcgtcaagct catctccaaa atgatgtccc gcacgggcat cacttccgtg cacgacgcct
    21961 tcggctcgcc caatgatctg caggcgtatc aggacgcgcg cgatgccggc gaactctctc
    22021 tgcgcgtcta ctgcctgatc ggttaccagt tcatcgacca gatgatcgcg gcgggcgtgc
    22081 gcaccggcct gggcgatgaa tgggtgcgcg tcggcgcgat gaaagcgatt tgcgacggct
    22141 cgtgctccga gcgcacggcg cgcctctcgg ctccctacgt cggccgcccc aacgacttcg
    22201 gtattttggt gatggacgaa gaggagatgt actcccacat gcacaaggcc cacgccgcgg
    22261 gctggcagat cggcacgcac gccaacggcg acgtggccat cgacaagaca ttgaaagtct
    22321 acgagcgcct gcagcgcgaa agtccgcggc gcgatccgcg cttccgcctg gagcactgca
    22381 ccgtgatcaa cga
//
LOCUS       DASYVO010000155         4376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_89168, whole genome shotgun sequence.
ACCESSION   DASYVO010000155 DASYVO010000000
VERSION     DASYVO010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89168"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /gene="cdd"
                     /locus_tag="VGR03_05275"
     CDS             <1..430
                     /gene="cdd"
                     /locus_tag="VGR03_05275"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1993721.1"
                     /translation="PMSPSETTASVTARRREELIAVARKARENAYAPYSRFNVGAALW
                     ARSRKIYKGCNVENATYGLTVCAERVAVLKAISEGERGFAAIAIVADTEKLTPPCGAC
                     RQILWEFCGDIEVILANLRGKVETYRLSELFPNPFEKKFL"
     gene            471..1913
                     /locus_tag="VGR03_05280"
     CDS             471..1913
                     /locus_tag="VGR03_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV1993722.1"
                     /translation="MKRAILFLLILLLGGFAARPTAARQRTKQAAGKTAEKIDLLVTG
                     GTVVTMDAPRRVIDDGAIAIRGDSIVAVGPRAELEARFPHPMRRIDARGRVILPGLIN
                     GHTHAAMVLLRGIANDLNLQDWLEKYIFPAEARNVTEDFVTWGTRLAALEMIRGGTTT
                     FVDMYYFEDAAARATKEAGMRGVLGESLIDFPVPDAKGPDEALVNIERFIKQWQGDGL
                     IHPAVAIHSPYTCSGETLKKGAALARKYSAPILIHVSETKREREESLAKKGMTPVAYL
                     DSLGFLGPDVVAAHCVWFDDKDIALAAQRGIGCVHNPSSNMMLASGTAPVVEMLRGGL
                     RLGLGTDGPAGSNNDLNMMEEIDLAAKLQKVTRLDPRALSAEQALEMATTGGARALHM
                     ENEIGSLEPGKKADLILLRTDAPHAVPMYDVVSQVAYALKASDVETVIVGGRILMQDR
                     RMLTLNEPEILAKAREYAEKVKRSLAPEKK"
     gene            2030..>4376
                     /locus_tag="VGR03_05285"
     CDS             2030..>4376
                     /locus_tag="VGR03_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV1993723.1"
                     /translation="MTDSSSMIGRTITHYRIVERIGGGGMGVVYKAEDTTLGRFVALK
                     FLPEELAKDRQALDRFQREARAASALNHPNICTIHEIGQQDGQAFIVMELLEGRTLRQ
                     QIAGKPLEIENLVGLGLEIADALDAAHAKGIIHRDIKPANIFVTQRGHAKILDFGLAK
                     LAPQRQLAAGIATASALPTLAADEPEHLTSPGTALGTVAYMSPEQVRGEDLDARTDLF
                     SFGAVVYEMATGRQAFPGNTSGVISHAILERAPTPVARVNPDAPPELERILNKALEKD
                     RKLRYQSASDLRTDLHRLERDTDSARVGALSGVVPTAGARPWWRRKTAVVAGGLTLAA
                     LLALGTWFTVFRARGEAIDSLAVLPFANASGDPNAEYLSDGITESIINSLSQISNLRV
                     MARTTVFRYKGKDIDPQKIGRELKVRAMVTGRVQQRGDNLIIQAELVDVDKGSQLWGG
                     QFNRKLADVIGIQEDISKEITEKLRLKLSGEEKQRLTKRYTENAEAYQLYLKGLYYLY
                     KSSEEGSLKAREYFQQAIDKDPGYALAYAGMAVVYVYQSGLPPREAMPKARAAAGKAL
                     ALDDRLAEAHVSMGLVNLWYDWDWAAAGKHLERAIELNPAYPLAHFEHANYLAALGRP
                     EEALAERRRTLELDPVTPLFNSGMANQLYLARRFDEAIEQDRKTLELDPSFPGSHFGL
                     GFSYAAKGMYREALAEFAKLPRGWGDPFVAYAHGRLGERSQALRILDRLKAVSKQEPV
                     HADAFAVIYLGLGEKDQTFAWLEKAYEEHDLIFLSTLKLDPL"
BASE COUNT          918 a         1282 c         1384 g          792 t
ORIGIN      
        1 gccgatgagt cccagcgaaa cgaccgcatc cgtgactgcc cgccgccgcg aggagctgat
       61 cgccgtcgcg cgaaaagccc gcgaaaacgc ctacgcgcca tattcgagat tcaacgtcgg
      121 cgcggctctg tgggcaagat cgagaaaaat ctacaaaggc tgcaacgtgg agaacgccac
      181 gtacggcctt accgtctgcg ccgagcgcgt cgccgtcctg aaagcaatct ccgagggcga
      241 gcgtggcttc gcagcgatcg ccatcgtcgc cgataccgaa aagctcactc cgccctgcgg
      301 cgcctgccgc cagatcctgt gggagttttg cggcgatatc gaagtgattc ttgcgaatct
      361 gcgcggcaag gtggagacgt atcgcctgtc ggagctgttc ccgaatccgt tcgaaaagaa
      421 atttctctaa aatggacgcg ttcgcccgag gcggcctccg aggcaaacaa atgaaacgtg
      481 caattctatt cttgctgatt ctcttgctgg gtggatttgc cgcgcgcccc accgccgctc
      541 ggcaacgcac gaagcaagcc gccgggaaaa ccgctgaaaa aatcgacctg ctcgtcaccg
      601 gcggtacggt cgtaacgatg gatgcccccc gccgcgtcat cgacgacggc gcgatcgcga
      661 ttcgcggcga ttcaatcgtt gccgtaggcc cgcgcgccga actggaagcg cgtttcccgc
      721 atccgatgcg gcgcatcgac gcgcgcggtc gtgtcattct tccgggcctc atcaacggcc
      781 acacgcacgc ggccatggtg ctcctgcgcg gcatcgccaa cgacctgaac ctgcaggact
      841 ggctcgagaa gtatattttt ccggctgagg cgcgaaacgt gaccgaagac tttgtcacct
      901 ggggcacgcg cctcgccgcg ctggagatga tccgcggcgg gacgaccacg tttgtcgaca
      961 tgtactactt cgaggatgcc gcggcccgcg cgacaaaaga ggccgggatg cgcggcgtcc
     1021 tcggcgagag cctcattgat tttcctgtgc cggacgccaa gggtcccgac gaagcgctgg
     1081 taaacatcga gcgattcatc aaacagtggc agggggatgg gctcatccac ccggcggtgg
     1141 cgattcattc gccatacacc tgctcgggcg aaacgttgaa gaagggagcg gcgctggccc
     1201 gcaaatattc tgcgccgatt ctgattcacg tatcggagac caagcgcgaa cgcgaggaaa
     1261 gcctggcaaa aaaagggatg acgccggtgg cgtatctcga ttcccttggt tttctcgggc
     1321 cggatgttgt ggccgcgcat tgcgtgtggt tcgacgacaa ggacatcgcg cttgcggcgc
     1381 agcgcggcat cggctgcgtt cacaaccctt cgagcaacat gatgctggcg agcggcacgg
     1441 caccggtggt ggaaatgctg cggggcggcc tgcgattggg tctgggcacc gacggcccgg
     1501 cgggcagcaa caacgacctg aacatgatgg aagagatcga cctcgcagcg aagctccaga
     1561 aagtgacgcg gctcgacccg cgcgctctct ccgccgagca ggcgctggag atggccacca
     1621 ctggcggggc ccgcgcgctg cacatggaaa acgaaatcgg atcgctcgag ccggggaaaa
     1681 aagccgacct catcctgttg cgcaccgacg cgccgcacgc cgtgccgatg tacgacgtgg
     1741 tctcgcaggt cgcctacgcg ctcaaggcca gcgacgtgga aacggtgatc gttggtggcc
     1801 gcatcctaat gcaagatcgc cgcatgctca cgctgaatga gccggaaatc ctggcgaaag
     1861 cgcgcgagta tgccgaaaag gtgaagcgct cgctggcgcc ggaaaagaaa tgaaggccac
     1921 aggtagtggg tccgcttccg gtttgccgac tccacgcaga tgtggacgct ggccagccta
     1981 tgttgcacga ttgcgtgtat cgcggcgctg ggcgtaacat gcacctccag tgaccgattc
     2041 ctcttccatg attggccgca cgattaccca ctaccgcatt gtagagagga ttggtggcgg
     2101 cgggatgggt gtggtgtaca aggccgaaga caccactctg ggccgtttcg tcgcgctcaa
     2161 atttctcccg gaagaactgg cgaaagaccg gcaggcgctg gatagattcc agcgcgaagc
     2221 tcgcgctgcc tcggcgctga accatcccaa catctgcacg attcatgaga ttggtcagca
     2281 ggatggccag gccttcatcg tgatggaatt gctggaaggc cgcacgctcc ggcaacaaat
     2341 cgcgggcaag ccgctggaaa tcgagaatct cgtgggtctt gggctggaga ttgccgatgc
     2401 actggatgcc gcgcacgcca agggaattat tcaccgggac atcaagccgg ccaacatttt
     2461 cgtgacgcaa cgtggtcatg ccaagattct agattttggc ttggcgaagc tggcaccgca
     2521 gcgacaactg gccgccggga ttgccacggc ctccgcgttg ccgacgcttg ctgccgacga
     2581 accggagcat ctcaccagcc ccggcacagc tttgggtacc gtggcctaca tgtcgccgga
     2641 gcaagtgcgg ggagaagacc tcgacgcgcg caccgacctg ttttcctttg gcgcggtggt
     2701 ctatgagatg gccacggggc ggcaggcgtt ccccggcaac acttccgggg tcatctcgca
     2761 cgcgatcctg gaacgcgcgc ccacccccgt ggcgcgtgtg aaccccgatg cgccgccgga
     2821 gctggagcgc atcctcaaca aggcgctgga aaaagaccgc aagctgcgct accaaagcgc
     2881 ttccgatcta cggacggact tacaccgact ggagcgggat acggactcgg cccgcgtcgg
     2941 tgcgctgagc ggggtggtcc cgaccgcagg agccaggccg tggtggcgca gaaaaacggc
     3001 tgtggtggcg ggcggactca ctttggcggc gctgctggcg ctgggaacgt ggttcaccgt
     3061 ctttcgcgcc cggggcgagg ccatcgattc gctggccgtg ctgcccttcg ctaacgcgag
     3121 cggtgatccc aatgccgaat atttgagcga cgggattacg gagagcatca tcaacagcct
     3181 ctcgcagatt tccaatcttc gcgttatggc gcgaacgacg gtgtttcgct acaaagggaa
     3241 agatatagat ccacaaaaga tcgggcggga actgaaagta cgcgcgatgg tgacgggcag
     3301 agttcagcaa cggggcgaca acctgatcat ccaggccgag ctggtggatg tcgataaggg
     3361 gtcgcaactt tggggcggcc agttcaaccg caaactcgcg gatgtgatcg ggatccagga
     3421 ggatatttcc aaggaaatca ccgaaaagct gcggctcaaa ctgagcggtg aggaaaagca
     3481 gcggctgacg aaacgctaca cggagaatgc cgaagcctat cagctgtacc tcaagggact
     3541 ctactacctg tacaaatcga gcgaagaagg atcgctaaag gctcgtgaat atttccagca
     3601 ggccattgac aaggacccag gctacgcgct ggcttacgcg gggatggcgg tcgtctacgt
     3661 ttatcaaagt gggcttcctc ctcgggaggc gatgccgaaa gcaagggctg cggccgggaa
     3721 ggcgttggcg ctcgatgacc gcctcgccga ggcccatgtt tccatgggat tggtcaacct
     3781 ttggtacgat tgggattggg cggctgccgg aaaacacctt gagcgagcca tcgagctgaa
     3841 tccagcgtat cccctggccc attttgagca tgcaaactat cttgccgctc tcggacggcc
     3901 ggaggaagcc ctggcagaga gaagacgcac cctggaactc gatccggtta ccccgttgtt
     3961 taactctggc atggcgaacc aactttacct tgcgaggcga ttcgatgagg cgattgaaca
     4021 ggaccggaag acgctggagc tcgatccgag ctttcccggt tctcattttg gattggggtt
     4081 cagctatgcg gcaaagggga tgtaccgaga ggctctggcc gagtttgcaa agctcccgcg
     4141 aggttggggc gacccttttg tggcgtacgc gcatggccga ttgggtgagc gaagccaggc
     4201 gctccggatt ctcgacaggc tcaaggcagt ctcaaaacaa gagcccgtgc atgccgatgc
     4261 attcgctgtc atttatctcg gactggggga gaaagatcaa accttcgcgt ggctcgaaaa
     4321 ggcttacgag gagcacgact tgattttcct cagcacgctt aaactggacc cactct
//
LOCUS       DASYVO010000156         9634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_244143, whole genome shotgun sequence.
ACCESSION   DASYVO010000156 DASYVO010000000
VERSION     DASYVO010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9634
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244143"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VGR03_05290"
     CDS             <1..481
                     /locus_tag="VGR03_05290"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEV1993724.1"
                     /translation="EMEGEPEFRKHVGYSAFVEGWALYSESLGEELGLYKDPYSKFGQ
                     LSYEMWRAVRLVVDTGMHSMGWTREQAIQFFKDNTGKTDQDITVEVDRYIVWPGQALA
                     YKIGQLKIRELRAEAEKKLGAKFDVRKFHDGVLEDGAVPLSVLEGHMKTWIEGQAGH"
     gene            759..1979
                     /locus_tag="VGR03_05295"
     CDS             759..1979
                     /locus_tag="VGR03_05295"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1993725.1"
                     /translation="MLALSDNLVQCFSALRAHKLRASLTMLGLTMGVATLITVMTIVQ
                     GANVYVEQKIANLGTNVFQVARTPFAATDFNIIIKALKYKKIELEDMRAVAEGCPACQ
                     EIGATASGSVHARYANKEVADVNFYGQTPSMADIDTRTVEFGRYFTAAEAEHRADVCL
                     IGDTLVQQLFLGMDPIGKSFRVGNDELMVIGVMEKIGSVLGQDQDNFITVPLPVFLRM
                     QGAHTSVTINVKTYTGNFEKAQDQADLVMRARRHLSGKMEDDFFIGTKESYMALWRSI
                     SSAFFGVFILVSAISVVIGGIVIMNVMLVSVNLRRREIGVRRAVGATKRDILRQFMAE
                     SVMQCIAGGALGISLGFLVALALRTYTPFPAAVQTWVAVLGVFLSSGVGLFFGIYPAV
                     RASQLDPIVALRSD"
     gene            1979..3244
                     /locus_tag="VGR03_05300"
     CDS             1979..3244
                     /locus_tag="VGR03_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1993726.1"
                     /translation="MAIMTLAQTRENFLAALETLRLSKVRSALTVLGIVIGVSSVISM
                     AAIIQGLNKFVQDRVERLGSRTYFISRFPFGTDPSRLPERIRTRKYLEYNYAEFVREA
                     APDVRIVTTFGTRGFFFGDSNRIVSGDRSVEKVVIRGAEPEYTEAIPLFRIERGRFIS
                     AFDQEHARPVVVLGAAISESLFPNTDALGKTVRINGGAYEVIGVFEHDQGLFLGPGVD
                     IFVIIPLSNFKKRYPEAKELFMAFTIPENVNVETAHGEVIQALRRLRRIPAGKENDFE
                     LNSPDFLSNLWNQLTGALVILTTVISSIGLLVGGIGVMNIMLISVTERTQEIGVRKAI
                     GARRADVRLQFLLEAVVLTLVGGSIGILIGAGVSTLVRTLVPSIPATLSYLWVSIGFA
                     ISVGVGLFFGYYPANLAANLDPIVCLRYE"
     gene            complement(3886..5139)
                     /gene="rho"
                     /locus_tag="VGR03_05305"
     CDS             complement(3886..5139)
                     /gene="rho"
                     /locus_tag="VGR03_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEV1993727.1"
                     /translation="MSLAELKEKNITDLAKIAKEHNLPGASGMRKQELIFQILRAQTE
                     KSGLIFSEGVLECLPDGFGFLRAPEYNYLPGPDDIYVSPSQIRKFDLRTGDTVSGQVR
                     PPKDGERYFALIKVEAVNFEDPEVARNKIFFDNLTPLYPQGRLKMETTKENMTGRVLD
                     LLCPVGKGQRGLVNAPPRTGKTMMLQAMANAVAINHPDVALIVLLIDERPEEVTDMQR
                     TVKGEVISSTFDEAPQRHIQVAEMVLEKAKRLVEHKRDVVVLLDSITRLARAYNAVTP
                     PSGKVLSGGIDANALQRPRRFFAAARNVEEGGSLTIIATALIDTGSRMDDVIFEEFKG
                     TGNMEIYLDRRLADKRVFPAIDIQRSGTRKDELLLPPDELSRIWVLRKVLSPLSTVEA
                     MELLISRLSKSKANMEFLASMTAPT"
     gene            complement(5406..7814)
                     /gene="lon"
                     /locus_tag="VGR03_05310"
     CDS             complement(5406..7814)
                     /gene="lon"
                     /locus_tag="VGR03_05310"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:NF008053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEV1993728.1"
                     /translation="MFTREKQEMKRVPMMPVRDMVIFPQQMTPFIVGREASVRALEEA
                     LAGDKKIFLSTQHDASVDDPKPEEIYAVGTLANIVQSVKLPDGNIKVLVEGVERARAL
                     SIATEEGFFRATVRLLGARVEPSPQVDQTVQKITGLYEQFIKLSQSLNYDTMIAAARV
                     DDPARLSDTIAANLQLPVDEKQDLLETVDPVERLNRIGDILEIELEKLNVDRNINTRV
                     KRQMERAQKEYYLNEKLKAIQKELGRGEKNEIDELKKKIDTAGMSKEIHEKAIQELKR
                     LELMPPMSAESTVSRNYLDWLLAVPWKKRSKEIRDIKAAEKILNEEHYGLDKVKERIL
                     EFLAVRQLVKNPKGSILCFVGPPGVGKTSLAMSIGHATGRKFVRVSLGGVRDEAEIRG
                     HRRTYIGALPGQVIQMMKKAGTVNPIFVLDEVDKMSTDFRGDPSAALMEVLDPELNHS
                     FTDHYLDVEYDLSKVMFVCTANVLHTIPQPLMDRMEILRLPGYTEQEKLEIAKRFLVK
                     RAREATGLTDKNIKFTDEGLLHIIRHYTHEAGVRSLEREIQNIARKMARKVVTDGASS
                     NVEITPVNANDFLGVLKYREFWLEKHNEIGLTTGLAWTEVGGSVLSTEATLMEGKGRL
                     TLTGKLGDVMQESAQAAMSYVRSRSNLLGLPRDFYRNIDIHVHVPEGAIPKDGPSAGI
                     TICTSIVSALTKNPVRRDVTMTGEITLRGKVLPIGGVKEKLLAAHRMGLRTILLPKDN
                     EKDLAEIPQEILASLTMHFVETMDEVLQIALERPTVPLEHAASAPVAEPFVAGAEKDK
                     SLTN"
     gene            complement(7882..9132)
                     /gene="clpX"
                     /locus_tag="VGR03_05315"
     CDS             complement(7882..9132)
                     /gene="clpX"
                     /locus_tag="VGR03_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV1993729.1"
                     /translation="MKKFNPDEPLRCSFCHKTQEQVEKLISSPSDYPRAYICNECVGV
                     CQTILEDEKREQAAAPTKRLPRPPEIKQFLDGYVIGQDRTKKKLAVAVYNHYKRIFLN
                     KQPGEIELSKSNILLIGPTGTGKTLLAQTLSRMLDVPFAIVDATTLTEAGYVGEDVEN
                     IILKLLQAADGDVQKAQTGIIYIDEIDKIAKKDENPSITRDVSGEGVQQALLKILEGT
                     VANVPPQGGRKHPHQEFTPVDTTNILFICGGAFVGLEKIIERRAGRRSLGFHTDAAPT
                     TPQRRNTEMLEQVQPGDLIRYGLIPEFVGRLPVAGVMNDLDKGTLVKILTEPKNALLK
                     QYQRLFEFENVRLRFTDDALETVAELALQRKVGARGLRMILEDLMLELMYHLPMQRKV
                     RDFVVTADMVRTREINWSLLEKAG"
     gene            complement(9296..>9634)
                     /locus_tag="VGR03_05320"
     CDS             complement(9296..>9634)
                     /locus_tag="VGR03_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEV1993730.1"
                     /translation="VKPDVVTTCVGQAASIAAVLLAGGAAKKRFALPNARILIHQPWM
                     SGLGGQATDIDLHAKEILRMRSTINTLLAKHSGQPVDKIEKDVERDYIMNPEQAKEYG
                     MIDEIIYKHR"
BASE COUNT         1875 a         2721 c         2884 g         2154 t
ORIGIN      
        1 agaaatggag ggagaaccag aattccgcaa acatgttggc tactcggcgt tcgtcgaagg
       61 ctgggcgttg tattcggaga gcctcggcga agagctcggc ctgtacaaag atccatactc
      121 gaagttcggg cagctcagct acgaaatgtg gcgtgccgtg cggctcgtcg tggacaccgg
      181 gatgcactcc atgggctgga cgcgcgaaca agcgattcag tttttcaagg acaacaccgg
      241 aaaaaccgac caggacatca ccgtcgaagt ggatcgctac atcgtgtggc ccgggcaggc
      301 gctggcgtac aagattgggc agttgaagat tcgcgagctg cgcgcagaag ccgagaagaa
      361 acttggcgct aaattcgatg tgcgcaagtt ccatgatggg gtgcttgagg acggcgcggt
      421 gccgctgagt gtgctggaag ggcacatgaa aacgtggatc gaggggcagg cgggtcacta
      481 gccgcctgcc gggactcgct tcggccccga aagaaccctc cgaagccaag aacggcctgc
      541 gctccggaca ggcgcggcag cggtcctcgc agaaaaccgt tacctttaga gtgtgacaaa
      601 ccgcgaagag tccggcaccc gctgaatgag gttccgggct ctgtaactcc cgcacccctt
      661 ccttcgtatt cttatagagg tgattcctgt gtcgcccgcg agctggcggt gcgcgcgcga
      721 aggatttggt aaagtgagtg ggggtctact gcaagcctat gctcgcactg agtgacaatc
      781 ttgtgcagtg cttcagcgcc ttgcgcgcgc acaagctgcg cgcctcgctc accatgctgg
      841 ggctgacgat gggtgtggcc acgctcatca cggtcatgac catcgtgcag ggcgcaaacg
      901 tgtacgtcga acaaaaaatc gccaacctcg gcacaaacgt ctttcaggtc gcgcgcactc
      961 ccttcgcagc gaccgatttc aacatcatta tcaaggccct gaagtacaag aagatagagc
     1021 tcgaggacat gcgcgccgtt gccgaaggct gtcccgcttg ccaagagatt ggcgctaccg
     1081 ccagcggcag cgtgcacgcg agatacgcca acaaagaagt ggcggacgtg aacttctacg
     1141 ggcaaacgcc gagcatggcg gatattgaca cgcgcaccgt agagtttggc cgctacttca
     1201 cggcagcgga agccgagcac cgcgctgacg tctgcttgat cggcgacacg ctggtgcagc
     1261 agttgtttct cggcatggac ccgatcggga agagctttcg cgtcgggaac gacgaactca
     1321 tggtgatagg ggtcatggag aagatcggca gcgtccttgg gcaggaccag gacaacttca
     1381 tcacggtccc cttgccggtg ttcctccgca tgcaaggcgc gcacacgagt gtgaccatca
     1441 acgtcaagac gtacaccggg aattttgaga aagcgcagga tcaggctgac ctggtcatga
     1501 gggcgcggcg tcacctcagc ggaaaaatgg aggacgattt cttcataggc acgaaagaaa
     1561 gttacatggc cctgtggcgc agcatcagct cggcgttttt cggcgtgttc attctggtga
     1621 gcgccatatc cgtggtcatc ggcgggatcg tcatcatgaa cgtgatgctc gtgagtgtga
     1681 acctgcggcg aagggagatc ggcgtgcggc gcgcggtcgg cgcgacgaag cgagacatcc
     1741 tgcgacagtt catggcggaa agcgtcatgc aatgtatcgc cggcggagcc ttgggcatct
     1801 cgttagggtt tttggtggcg ctggcgcttc ggacgtatac gccattccca gcggcggtgc
     1861 aaacgtgggt ggcggtgctg ggagtatttc tgagttccgg agtcggattg tttttcggga
     1921 tctatccggc ggtgcgtgcg tcacagctcg acccgattgt ggcgttgagg tctgactaat
     1981 ggcgatcatg acgcttgcgc aaacccggga aaatttcctt gccgcgctgg agacgctgcg
     2041 cttgtccaaa gttcgctccg ccctgaccgt gttgggcatc gtcatcggcg tgtcctcggt
     2101 gatttccatg gcggccatca ttcaaggtct gaacaagttc gtgcaagaca gagtggaacg
     2161 cctgggctcg cgcacttatt tcatctcacg attcccgttc ggcacggacc cttcccgctt
     2221 gccggaacgg attcgaactc gcaagtatct tgaatacaac tacgccgaat ttgtccggga
     2281 ggccgcaccc gacgttcgga tcgtcacgac gtttggcacg cggggcttct ttttcggaga
     2341 cagcaaccgc atcgtcagcg gtgaccgcag cgtcgagaag gttgtcatcc gcggcgccga
     2401 gccggagtac acggaagcga ttccgttgtt taggatcgag cgcggccgct tcatcagcgc
     2461 tttcgatcaa gagcacgccc ggccggtcgt cgtgctgggc gcggccatca gcgaatcgct
     2521 ctttcccaac acggacgccc tcgggaaaac ggtgcgcatc aatggcggcg cttacgaagt
     2581 tatcggggtg tttgagcacg atcagggatt gttcctcggc ccaggcgtgg acatctttgt
     2641 gatcatcccg ctcagcaact tcaagaaacg gtacccggaa gccaaagagt tgttcatggc
     2701 gttcaccatt ccggagaacg tcaacgtcga aaccgcgcac ggcgaagtca tacaagcgct
     2761 gcgccgcctc cgccgcatcc ccgctgggaa ggaaaatgat ttcgagttga actcaccgga
     2821 cttcctcagc aatctctgga accagctcac tggcgcgctg gtgattctca ccacggtcat
     2881 cagttcgatc ggactgctgg tgggcggcat cggcgtgatg aacatcatgc tcatctcggt
     2941 gaccgagcgc acccaggaaa ttggcgtgcg caaagccatc ggcgcgcgcc gcgcggatgt
     3001 ccgcctgcag ttcttgctgg aagccgtggt gctcacactc gtcggcggat ccatcggcat
     3061 tctgatcggg gcaggtgtct ccacgctggt gcgcactctc gtgccttcga ttcccgcaac
     3121 gctgtcctat ctttgggttt cgattggatt cgcgatctcc gtcggggtgg gattgttctt
     3181 cggctattat ccggcgaacc tcgcggccaa cctcgatcct atcgtttgct tgcggtacga
     3241 atgaagagct tggagtgcca atggccggcg ctaggtactt tagaattaac aagttacgcg
     3301 gaaaattggc caaaaatgcg gtttcttgaa aatatttctt gacagatttt ccaaaaccgt
     3361 ttactttcga gatatctcag ttgtatacct gctgcatttt agtgcaggcc ctcactgagc
     3421 gtgaagagca gacgatgaga cacgaagaca aaagccaaga cggatcgatg acaaggcgca
     3481 agggtctcgt ggactgccta ttcgcgcgac cttaactaaa tccgcctaag tcatttcgat
     3541 tcaaagcatt tccccggggg atttgtgtcc cttaccgcgg gtgaatcgct cgaatactag
     3601 agttttcgtt gtgggagaag tgtttccaga aggaggtgaa cttgcgcgac gcgctttttc
     3661 tctgcaacgc gaagccgaaa gatgcggagc aggcggccgt ttggaaaaag ctcgttgaga
     3721 acacgttcag gcctccccat acctgggaag ctgtcttgtc cgcagggaag gacaagcgcg
     3781 agaatttcag gcggctcctg ccgcatcgtc cggtaatcgg cgcagcgggg gaaactcgct
     3841 gtgccctttt ttcgccctga ctgaaaataa gagattgtgg cgtcctcagg tcggtgcggt
     3901 catcgaggcc aaaaattcca tgttggcctt cgacttcgac agccggctga tcaacagctc
     3961 catggcctcg actgtcgaga gcgggctcaa aaccttgcgc agcacccaaa tgcggctcaa
     4021 ctcatccggt ggcaggagca gttcgtcctt tcgggtgccc gagcgctgaa tgtcgatggc
     4081 ggggaagacg cgcttatcgg cgaggcggcg gtcgagatag atttccatgt tgccggtacc
     4141 cttgaactct tcgaagatga catcgtccat gcggctgcca gtatcgatga gcgcggtggc
     4201 gatgatggtg agcgatccgc cctcttccac attacgggca gcggcgaaga agcgccgcgg
     4261 gcgctgcaag gcgttggcat cgataccgcc ggagagcact ttgccggaag gcggcgtgac
     4321 ggcgttatag gcgcgggcca gacgcgtgat ggaatcgagc agcacgacca catcgcgctt
     4381 gtgttcgacg aggcgcttgg ctttttccaa aaccatttcc gcaacttgga tgtggcgctg
     4441 gggcgcttcg tcaaacgtcg agctgatgac ttcgcccttc acggtgcgct gcatgtcggt
     4501 gacttcttcc gggcgctcgt cgatcagcag cacgatcagc gcgacgtctg gatgattgat
     4561 agcgacggcg ttggccatgg cttgcagcat catggtcttg ccggtgcgcg gcggcgcatt
     4621 gaccaggccg cgctggcctt taccgacggg gcagagcaga tccagcaccc ggccggtcat
     4681 attttccttg gtagtttcca tcttgaggcg gccctgggga tagagcggcg tcaagttatc
     4741 gaagaagatc ttgttgcgcg cgacttccgg gtcctcgaaa ttgacggctt cgactttgat
     4801 gagcgcgaaa taccgttcgc cgtctttggg cgggcgcacc tgaccggaga cagtgtcgcc
     4861 ggtgcgcagg tcgaacttgc gaatctgcga tggcgagaca tagatatcgt ccgggccggg
     4921 gagatagttg tattccggcg cgcgcagaaa gccgaatcca tcggggaggc actcgaggac
     4981 accttcggaa aagatcaacc cgctcttctc ggtctgcgcg cggaggatct ggaagatcaa
     5041 ctcctgcttg cgcatgccgc tggcgccggg aagattgtgt tccttggcga tcttggcgag
     5101 gtcggtgatg ttcttttcct tcagctcggc aaggctgata ctcatctgat tgtttccttc
     5161 aatgcttgtt tcttttggca tatgtcctta gctatgattg tgaaaattga ccctgagaat
     5221 ccggattgtt tttgaggtgg cgcaccgcac tggcggataa acgaaaacct gccagaatgg
     5281 ggcaaaccag actttctgca accggtagca gcgccgggac gcggcgaacc tgaaagttcc
     5341 tcaagcgtaa ctccgaccag gtcggggtca actcgcggga cgggacggac ccaaaacaac
     5401 gctgcttagt tggtcaaact cttgtctttc tcggcgccgg ccacgaaagg ttccgctacg
     5461 ggcgcacttg cggcatgctc aagcggcacc gtgggccgct ccagcgcaat ctgcagcact
     5521 tcatccatgg tctcgacaaa atgcatcgta agactggcga ggatctcctg cgggatttcc
     5581 gctaggtcct tctcattgtc cttcggaagc agtatcgtac gcaagcccat tcgatgggcg
     5641 gccagaagtt tctccttgac gcctccgatg ggaagcacct tgccgcgcag cgtgatttcg
     5701 ccggtcatgg tgacgtcgcg gcgcacggga ttctttgtca gcgcgctgac gatggacgtg
     5761 cagatggtga tgcccgcgga aggaccgtcc ttggggatgg ctccttcggg tacgtgtacg
     5821 tgaatgtcga tgttgcggta gaaatccctg ggcaggccga gtagattgga gcgcgaacgg
     5881 acgtaactca tcgcggcttg cgcggactcc tgcatcacgt caccgagctt gccggtcaga
     5941 gtgaggcggc ccttgccttc catcaatgta gcctctgtcg acagcacgga gccgccgact
     6001 tcggtccacg ccaagccggt ggtcaggcca atttcattgt gtttctcgag ccagaattcg
     6061 cggtacttca gcacgcccag gaagtcgttg gcgttgacgg gtgtgatttc cacatttgag
     6121 ctcgcgccgt cggtgacgac tttgcgggcc atcttgcgcg cgatgttctg aatctctcgc
     6181 tccaggctgc gaactcccgc ttcgtgcgtg taatgccgga tgatgtgaag aaggccttcg
     6241 tccgtaaatt tgatgttctt gtcggtcagg ccggtcgctt cgcgcgcgcg cttcacaagg
     6301 aaacgcttag cgatttccag cttttcctgc tccgtatagc caggaaggcg caggatttcc
     6361 atgcggtcca tgagcggctg cgggatcgta tgcagcacat tcgccgtgca cacgaacatg
     6421 actttcgaaa ggtcgtattc cacatccagg taatggtccg taaacgagtg gttcagttcc
     6481 ggatcgagca cttccatcaa cgccgctgac ggatcgccgc gaaagtctgt cgacatcttg
     6541 tcgacttcgt ccagcacgaa gatcgggttc accgttccgg ccttcttcat catctggatg
     6601 acttggccgg gcagggcacc gatataggtg cggcgatggc cacgaatctc tgcttcatcg
     6661 cgcacgccgc cgagcgaaac tcgcacaaac ttgcggcccg ttgcatgacc gatggacatg
     6721 gccagggaag tcttgcccac tcccggaggc cccacgaagc acagaatcga gcccttcggg
     6781 ttcttcacga gttggcggac cgccaaaaac tccaggatcc gttccttgac cttatcgagg
     6841 ccgtagtgct cttcgttcag gattttttcc gcggccttga tgtcgcggat ttctttcgat
     6901 ctctttttcc agggcacggc gagcagccag tcgaggtagt tgcggctgac cgtggactcc
     6961 gcggacatcg gcggcataag ttccaggcgc ttcagctcct ggatcgcctt ctcatggatt
     7021 tccttggaca tcccagcggt gtcgatcttc tttttcagtt cgtcgatttc gttcttttcg
     7081 ccgcggccca gttctttctg gatggctttg agcttttcgt tcaggtaata ctctttttgc
     7141 gcgcgctcca tctggcgctt cacgcgcgta ttgatgttgc ggtcgacgtt gagcttttcg
     7201 agctcgattt ccaggatgtc gccaatgcgg ttaagccgct cgacgggatc gacggtttcg
     7261 agcaaatcct gcttttcatc caccggcagt tgtaggtttg cggcaatcgt atccgagagg
     7321 cgcgctggat catccacgcg tgcggcggcg atcatggtgt cgtaattcag gctctgcgaa
     7381 agcttgatga actgttcgta gaggccggtg attttttgga cggtctgatc aacttgcggt
     7441 gatggctcga cacgtgcgcc cagcaagcga acagtggcgc ggaagaatcc ttcttccgtg
     7501 gcgatcgaca gggcccgagc gcgttccaca ccttccacaa gaaccttgat attgccatcg
     7561 ggaagtttga cactctgcac aatgttggcg agagttccca cggcgtagat ctcttccggc
     7621 ttggggtcat ccaccgaagc gtcgtgctgc gtggaaagga atatcttctt gtcgccggcc
     7681 agcgcttctt cgagcgcgcg gacgctcgcc tcacggccga cgataaaggg agtcatctgc
     7741 tgcgggaaga tgaccatgtc gcgcaccggc atcatgggca cgcgcttcat ttcctgtttt
     7801 tcgcgagtga acattcagtc ccctggattc tgctcgtgcg gccccggaac ggccgcacag
     7861 taagatcaca gcccgagcgg cttatccggc cttctcgagc aaactccaat tgatttcgcg
     7921 ggtgcggacc atgtccgccg tgaccacgaa gtcgcgcacc ttccgctgca tgggaaggtg
     7981 gtacatcagt tccagcatga ggtcttcgag gatcatacgc aagccgcggg cgccgacttt
     8041 gcgctgaagc gccaactccg cgacggtctc gagcgcgtcg tccgtaaatc gcaagcgcac
     8101 gttttcgaat tcgaacagcc gctggtattg cttcagcagg gcgttcttcg gctccgtcag
     8161 aattttaacc agcgtgccct tgtcgagatc gttcatgacc ccggcaactg gcaggcggcc
     8221 cacaaattcc ggtatcagtc cgtagcgaat caaatcgccg ggttgcacct gctcgagcat
     8281 ctcggtgttg cggcgctgcg gcgtggttgg cgcggcgtcg gtgtggaaac cgagcgagcg
     8341 gcggccggcg cgcctttcaa tgattttttc gaggcccaca aaagcgccgc cgcagataaa
     8401 taggatattc gtcgtatcca cgggcgtgaa ttcctggtgc ggatgcttgc gcccgccctg
     8461 cggaggcaca ttggccaccg tgccttcgag aatcttcagc agcgcctgtt gcacgccttc
     8521 tccggacaca tcgcgggtga tcgacgggtt ctcatccttc ttggcaatct tgtcgatctc
     8581 atcgatatag atgatgcctg tctgagcctt ctggacgtcg ccgtccgctg cttgcagcag
     8641 cttcagaata atgttctcga cgtcttcgcc cacgtaaccc gcttcggtga gcgtcgtggc
     8701 atcgacgatg gcgaaaggca catccagcat gcgcgagagc gtctgcgcca gcaacgtctt
     8761 gcccgtgccg gtggggccga tcaacagaat attggacttg ctgagttcga tttcgcccgg
     8821 ctgcttgttc aggaaaatcc gtttgtagtg gttgtaaacc gcgaccgcga gtttcttctt
     8881 cgtgcggtcc tgcccgatga cgtagccatc caggaattgc ttgatctccg gcgggcgggg
     8941 aagccgcttc gtcggcgcgg cggcctgttc gcgcttctca tcttccagaa tggtctggca
     9001 gacgcccacg cactcgttgc agatgtaggc gcgcggatag tccgaaggcg aactgatcag
     9061 cttttcgacc tgctcctgcg tcttatggca aaaggagcag cgcaaaggct catctggatt
     9121 gaattttttc actggtgtga aggcctcgtc tccgctgggc ttcgacggag tctctaacca
     9181 ctattctagg ccgattattc cccggagcga aagagaaagt cgaaaggcta ctaacttttt
     9241 ggaactggcg ctccatcccc gcttcacacc gcaaagcact ccgcggcgcg gaattctacc
     9301 ggtgcttata aataatctcg tcgatcatcc cgtattcttt ggcctgctcg ggattcatga
     9361 tgtagtcccg ctctacgtcc ttctcgattt tgtccaccgg ttgcccgctg tgcttggcca
     9421 gcagcgtatt gatggtcgag cgcatgcgca gaatctcttt cgcatgcaga tcgatgtccg
     9481 tggcctggcc gccgagaccg gacatccacg gctggtgaat caggatgcgc gcgttcggca
     9541 aggcaaatcg ttttttagca gcgccaccgg cgagcagaac ggccgcgatg gacgccgcct
     9601 gacccacgca agttgtcacc acgtccggct tcac
//
LOCUS       DASYVO010000157         5004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_271852, whole genome shotgun sequence.
ACCESSION   DASYVO010000157 DASYVO010000000
VERSION     DASYVO010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271852"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1221)
                     /gene="hpnH"
                     /locus_tag="VGR03_05325"
     CDS             complement(94..1221)
                     /gene="hpnH"
                     /locus_tag="VGR03_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEV1993731.1"
                     /translation="MPVPISQMWTVATYVLKQKLRGRQRYPFVLMLEPLFRCNLACAG
                     CGKIQYPAHILKTDLSPEECFKAVDECGTPMVSIPGGEPLMHPQIDKIVEGLVARKKY
                     IYLCTNALLLKEKLHLFKPSKYLTFSVHVDGQKEHHDFSVCREGGYDLAIEGIREAVK
                     QGFRVTTNTTLFDGADPKSVRAFFDEMMELGVEGMMLSPGYSYDKAPDQKHFLGRART
                     RRLFRAILSNRKASWQFNQSKLFLEFLMGKRTYACTPWGMPTYNVFGWQKPCYLLQDG
                     YTDTFQELLDTTEWQNYGTESGNPKCANCMVHSGYEASAVDHTFSGLRGLWATVKAEL
                     STKYNDADALAMLNEPAKPVHSYNPLVQIESGKGQFEETRA"
     gene            1504..4086
                     /locus_tag="VGR03_05330"
     CDS             1504..4086
                     /locus_tag="VGR03_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV1993732.1"
                     /translation="MPRKNLLSLFADFARFAGDAAVIQRRGYRREKLTYAELYARVLF
                     WSHALAGRGVVPGDRVLLWGPNSAEWVACFWGILLRGGVVVPMDAAAAPDFVQRAIKD
                     AGVKLILRDRQQMELPGAPPSMIINDFKDVRDSPAPKADAGVDLGDASIRSTIAEILY
                     TSGTTAEPRGVVLTHGNFLANLEPLERGIDEYRKYERWFHPLRFVTLVPLSHVFGQFM
                     ALFVPPLLGAAVAFEPSSNPAEIIRTIKKERATALIAVPRMLDLLHAGIEREFEGQGK
                     SQWLSRTLENAKGRKFLKRAWMFRRIHRRFGWKFWALISGGAALSKETEDFFKRAGYA
                     VVQGYGMTETASLISLNHPFHATEGSVGKILPGRECKLAEDGEILVRGENVSSGYWEQ
                     GTFRPADQESWLRTGDLGELDAEGNLRFRGRKKNVIVTPAGLNVYPEDLESALRKQPG
                     VRDCVVIPLGRGGNAEPCAILLLNNADDSGARAAIEGANASLTEYQRVRTWSVWPEFD
                     FPRTPTGKPRLSVITARAAQILDGRQDVHPEGVGADFSPPSASRSAFDQLLERFSTTR
                     GGGPRVDRHLEQNLNLSSLDRVELLSALEERFHVELNETVFANAKTAADVERILQQPA
                     ARRTEYIYPRWTQREPLRWLRLAIYYALVWPATQILGHPRIVGRENLRSLRGPVLIVS
                     NHITRRADIGLILAALPPRYRHRLATAMGGESLQNMRRPPRDWFFARRWGYQLGYWLA
                     TMLFNVFPLPQFSGFRESFRFAGESADRGYSVLVFPEGEVNNSEDGRMAPFQSGIGLL
                     AENLRIPIIPMRLDGVWQMKREHRRLAHFGEITVHIGAPVTFSPGTPADEIARRLESL
                     VRSL"
     gene            4256..>5004
                     /locus_tag="VGR03_05335"
     CDS             4256..>5004
                     /locus_tag="VGR03_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993733.1"
                     /translation="MAHRTTLEEERPAKTPKAVKKLAAGGLAKAAGNEERERVFDAFR
                     RWGYLEATLDPLGLFEPLKQPELQALTGEAAEEARGIYCDAVGADFMHLPEPERRRWI
                     AQRLEAPASEVDQHRILERLIRADLFEQVLQARYLGTKRFSLEGVTALIPLLDSILDT
                     AGEHGAVESVMAMSHRGRLNVMVHAACKTPHEVVAGFEDVDPRSVLGAGDVKYHVGAT
                     GTYVTSTGKEIGIHLVSNPSHLEAVDPVAVGR"
BASE COUNT          985 a         1557 c         1419 g         1043 t
ORIGIN      
        1 tttcatgaag ggttccgggg gccacttcca gcgtgtcggg gtttagcgga gaaccttccg
       61 tgtggcttgg ccggtccgcg tggcgcggac ccgtcacgcg cgcgtctcct cgaattggcc
      121 cttgccggat tcgatctgca cgagaggatt gtaggagtgt accggcttcg ccggctcatt
      181 cagcattgcg agcgcatccg catcgttgta tttggtggac aactccgcct ttaccgtcgc
      241 ccagagtcca cgcaggccgc tgaaagtatg atccacggcc gaagcttcgt aaccactgtg
      301 caccatgcag ttcgcgcact tgggattgcc ggactcggtg ccgtagttct gccactcggt
      361 ggtgtcgagc aactcctgga acgtgtccgt gtacccgtct tgcagcaggt agcagggttt
      421 ctgccagccg aagacgttgt atgtcggcat gccccacggc gtgcaggcat aggttcgctt
      481 gcccatcaga aactcgagga acagcttcga ctggttgaac tgccagctcg ctttgcgatt
      541 cgacaaaatg gcccggaaca agcgccgcgt gcgagcccgc ccgagaaaat gcttttggtc
      601 gggtgccttg tcgtaggaat agcctggtga aagcatcatg ccttcgacgc ccagctccat
      661 catttcgtcg aaaaacgcgc ggacactctt cggatcggcg ccgtcgaaca acgttgtgtt
      721 cgttgtgacg cggaagccct gtttgaccgc ttcccggatg ccttcgatgg ccagatcgta
      781 gccaccctcg cggcaaacgg aaaaatcgtg atgctctttc tgcccgtcga catgcacgga
      841 gaaggtcaga tatttgctgg gcttgaaaag atgcagtttt tccttcagta gcagggcgtt
      901 ggtgcagaga tagatgtact tcttccgcgc gaccaggccc tcgacgatct tgtcgatctg
      961 cgggtgcatc agcggttcgc caccgggaat cgaaaccatc ggcgtgccgc actcatccac
     1021 ggccttgaag cattcttccg ggctcaagtc cgttttcaaa atgtgtgcgg gatattggat
     1081 tttgccgcac cccgcgcaag ccaggttgca gcggaacaac ggctccagca tcagcacgaa
     1141 cgggtagcgc tgtcgccccc tcagcttttg cttgaggacg tacgttgcaa ccgtccacat
     1201 ctgtgaaatc ggtaccggca tctttcctgc ttatctccaa gaatggatag ggatgtaccc
     1261 aggccctcag gatctcctcg tacaacggaa accgcaagtc accattgtaa ccgagaatcc
     1321 gcgaatttcg ctcggcggct ttcagttaga tgctaggatg tgttccttcg tggcacggta
     1381 agtgcacttc ggtatcggat gcttcctcac gcgcaatcgg actgtctggc tggcccggaa
     1441 ggctatgccg acgcttcagg cgtagaatcg acccatatcc gaggcaaaga aactgccgca
     1501 cttatgcccc gaaaaaatct cctgagtctt ttcgcagatt tcgcacgctt cgccggcgat
     1561 gccgctgtca tccaaagacg cggctaccgt cgggaaaagc tgacgtatgc ggagctttac
     1621 gcccgcgtcc tcttctggag tcatgcgctc gccgggcgcg gggtcgtccc aggcgatcgc
     1681 gtgcttctct ggggccccaa ctccgccgag tgggtcgcct gcttctgggg aatcctcttg
     1741 cgtggtggcg tcgttgtccc gatggatgct gctgcggcgc ccgatttcgt gcaacgcgcg
     1801 atcaaggatg ccggcgtgaa gctcatcttg cgtgaccgtc agcagatgga gctgcccggc
     1861 gcgccaccat cgatgatcat caacgatttc aaggatgtta gggacagccc tgcccctaag
     1921 gcagatgccg gcgttgattt aggcgatgcg tccatacgaa gcaccatcgc cgaaatcctt
     1981 tacacttcgg gcacgacggc ggagccgcgc ggggtcgttc tcacgcacgg caattttctt
     2041 gcgaatcttg agccgctgga gcgcggtatc gatgagtatc gcaagtacga gcgctggttc
     2101 cacccgctgc gcttcgtcac cctcgttcca ttgagccacg tcttcggcca attcatggcg
     2161 ctgttcgttc caccgcttct cggagccgcg gtcgctttcg aaccgtccag caaccccgcc
     2221 gaaatcattc gcaccatcaa gaaagaacgc gccaccgcgc tcatcgccgt tccgcgcatg
     2281 ctcgatcttc ttcatgcggg aatcgagagg gaattcgaag gccagggcaa atcgcaatgg
     2341 ctgagccgta cccttgaaaa tgccaaaggc agaaaatttc tcaagcgcgc ctggatgttt
     2401 cgccgtattc atcgccgctt tggctggaaa ttttgggcat tgatctccgg tggcgcggcg
     2461 ctttcgaagg aaaccgagga ctttttcaag cgcgcgggtt acgccgtcgt tcaagggtac
     2521 ggtatgacgg agaccgcctc tttgatcagc ctgaatcacc cgtttcacgc gacggaaggt
     2581 tccgtcggca agattcttcc gggccgtgaa tgcaagctcg ccgaagacgg tgaaattctc
     2641 gttcgtggtg aaaatgtttc gagtggctat tgggaacaag ggacttttcg tcctgcagat
     2701 caggaaagct ggctgcgcac gggcgatctc ggcgaactcg acgccgaagg caatcttcgt
     2761 ttccgcgggc gcaagaaaaa cgtaattgtc acacccgccg gcctaaacgt ttaccccgaa
     2821 gacttggaat ccgctcttcg caaacagcct ggcgtgcgcg attgcgtggt catcccattg
     2881 ggtcgcggcg ggaatgccga gccctgcgcg atcctccttt tgaacaatgc tgatgattcc
     2941 ggcgcgcgcg ccgctatcga aggcgctaac gcctcgctca ctgaatatca acgcgttcga
     3001 acctggagcg tctggcctga atttgatttt cctcgcacac ccacgggcaa gccacgactt
     3061 tccgtcatca ctgcccgggc cgctcaaatt ctcgacggac gacaagatgt tcatccggaa
     3121 ggtgtagggg cggactttag tccgccctcc gcgtcacgta gcgcgttcga tcaacttctg
     3181 gaacgcttta gcacaaccag aggtggaggc ccgcgagtcg atagacacct tgaacagaat
     3241 ctgaacctct cctccctcga ccgcgtggag ctgctgagcg ccctggagga aaggtttcac
     3301 gtggaactga acgaaaccgt tttcgccaat gccaagaccg ctgccgatgt cgagcgcatc
     3361 ctgcaacagc cggccgctcg ccgcaccgaa tacatctatc cgcgctggac gcagcgcgaa
     3421 ccccttcgct ggctgcggct tgccatctat tacgctctcg tctggcccgc cacgcagatc
     3481 ctcggccacc cgcgcatcgt cggccgcgag aatcttcgta gcttgcgtgg gcccgtccta
     3541 atcgtttcaa accacatcac gcgccgcgcc gacatcggcc tgatcctcgc cgcgttgcca
     3601 ccgcgctata gacaccgcct cgccacagcc atgggcggcg aatcgttgca aaacatgcgc
     3661 cgcccgccac gtgattggtt cttcgccagg cgctggggct atcaactcgg ctactggctc
     3721 gccaccatgc tgttcaacgt ttttccgctg ccgcaatttt ccggcttccg cgaaagcttc
     3781 cgcttcgccg gcgaatccgc cgatcgcggc tacagcgtgc tcgtctttcc cgaaggcgaa
     3841 gtcaacaatt ccgaagatgg gcgcatggct ccttttcaga gcggcatcgg actcctcgca
     3901 gaaaatcttc gcatcccgat cattccgatg cgcctagacg gcgtctggca gatgaagcgc
     3961 gaacaccgcc gcctcgccca cttcggcgag atcaccgtcc acatcggcgc cccggtcacc
     4021 ttttcgcccg gcacgcccgc cgacgaaatc gcccgccgcc tcgaatcgct ggtgcgctct
     4081 ttgtaggggc gcagcatgct gcgcccctaa cgccgaaaac cgctccatcg cgaagactct
     4141 gcaattcagc gcctcctaag tgcatccaaa aacatatgtg agcaaagcgc cgcgcacgcc
     4201 cgcaagcaac ccattggctt ctgtggcaaa atagaaggtt catagagttg gatttatggc
     4261 acaccgtaca acactggaag aagagcgccc cgcaaaaacg ccgaaggcgg tcaagaaact
     4321 cgcggcaggc ggcctcgcga aagcagccgg caacgaagaa cgcgaacgcg tgttcgacgc
     4381 gttccggcgc tggggctacc tggaggcgac tctagatccc cttggcttgt tcgagccact
     4441 gaagcaacct gaactgcaag cgctgaccgg cgaagccgcc gaggaagccc gcggcattta
     4501 ctgtgatgcg gtcggcgccg atttcatgca tttgccggag ccggaacgcc gccgctggat
     4561 tgcgcagcgc ctcgaagcgc cagccagcga agtcgatcag cacagaatcc tcgagcgtct
     4621 gattcgcgcc gatcttttcg aacaggtctt gcaggcacgc tacctcggca ccaaacgctt
     4681 ctcgctcgaa ggcgtcacag cgctgattcc gctgcttgat tccattctgg acacagcggg
     4741 cgagcatggt gcggtggaat ccgtcatggc catgagtcac cgcgggcgcc tcaacgtaat
     4801 ggtccacgcg gcatgcaaga cgccacatga agtggttgct ggttttgaag acgtcgatcc
     4861 tcgcagcgtc ctcggcgcgg gcgacgtgaa ataccacgtg ggtgcgacgg gcacctacgt
     4921 cacgagcacc ggcaaagaaa tcggcatcca tcttgtttcg aatccaagcc atctggaggc
     4981 tgtcgatccg gtcgccgtcg gccg
//
LOCUS       DASYVO010000158         6363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_2854, whole genome shotgun sequence.
ACCESSION   DASYVO010000158 DASYVO010000000
VERSION     DASYVO010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2854"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1756
                     /locus_tag="VGR03_05340"
     CDS             <1..1756
                     /locus_tag="VGR03_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV1993734.1"
                     /translation="GTQFVQAVGAAEATLYYEGRPKALAQAKKAPLGEFVNHEREEIV
                     YVSSGDGATSEGEFWESLNVACAKKLPLLYLVEDNGYAISVPVEVQTAGGSISKLVRG
                     FPALHVAECDGTDPIESYSVCKEAVQYCRERRGPALVHAHVTRPYSHSLSDDEKLYKS
                     AEEREEEARRDPISKFALFLVREGILDQKQIESLEAQIDNEVRDAADQALAAEEPSID
                     SIRLNVYSPDVDPTSAAFEAEPQLHGAPKTMVEMVAATLSDEMARDERISVFGEDVAD
                     ASREENLRHVKGKGGVFKATAGLQRKYGSDRVFNTPLAEASIVGRALGMATRGLKPVA
                     EIQFFDYIWPAMMQIRDELCVMRWRSNGAFKAPIVIRVPIGGYLTGGGVYHSQSGESI
                     FAHCPGLRVVMPSTALDVAGLLRTAIRCDDPVMFLEHKHLYRQPYNRSENPGPDFTIP
                     FGKARMVKEGSDLSIITYGAVVHRAEVAAAQLEREGISVEIIDLRSLSPYDWEAIAAT
                     VRKTHRVIVAYEDMLSWGYGAEIAARIADELFEELDAPVRRVAAMDTFCAYQPKLEDV
                     ILPQTSHIAAAVKQLLEY"
     gene            1858..2190
                     /locus_tag="VGR03_05345"
     CDS             1858..2190
                     /locus_tag="VGR03_05345"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1993735.1"
                     /translation="MTCVNCQKDIAVGSRFCYNCGTKQPETPAPGLSPETGGKRKLMR
                     SGTDKKIAGVCAGLADYFDLDPTIVRVVWLLAILFAGTGVLAYLVLWIVLPVAPAGVI
                     PTSATVVS"
     gene            complement(2316..2882)
                     /locus_tag="VGR03_05350"
     CDS             complement(2316..2882)
                     /locus_tag="VGR03_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993736.1"
                     /translation="MFWIIPNFAAVSAHTEVAPLTTRGKFVLATQDSGDYSSFIWSGL
                     QAGQSMALRSYPELHNGLAGYGRYYWRAFADQASGSFFTEALVPAVTHEDPRYYTLGY
                     GGFFRRAGYALSRVVVTRTDSGGNTFNISEIAGNGMEAGLANLYYPPEERSLHNTVVN
                     WAAQLEAASLNNLIREFWPDIRRKLRQK"
     gene            4210..4917
                     /locus_tag="VGR03_05355"
     CDS             4210..4917
                     /locus_tag="VGR03_05355"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEV1993737.1"
                     /translation="MKRYLVIAVCLSVAAGAAYAATKEQKRLENCGVVLQEILNIPDN
                     LPQQVLAKAECVIVFPSVLKLAFGMGKDYGRGAMVCRTGKEFNGPWGAPAMYALEGDS
                     IGMQLGGESTDLILLVMNQKGAASILSSKIKVGGDASAAAGPKGRDASANTDAWMHAE
                     ILSYSRSRGLFAGISLEGSTLRPDNKANARIYGRKITARAILLGNKVAIPATARHLVR
                     VLQTNSPRNESKQVASR"
     gene            complement(5124..5564)
                     /locus_tag="VGR03_05360"
     CDS             complement(5124..5564)
                     /locus_tag="VGR03_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV1993738.1"
                     /translation="MKSLNSFQPLGLLVLRVALGLIFFSHGYPKLAHSAAGMQGFFVQ
                     HGLPGYFVYISGVLEVFGAVLLVLGLFTRAAALLLAIEMGVAIWKVHSTGGYLAVHDY
                     EFPLAVLAGSFALATVGAGLLSLDHPLYEGGGKSRPPKYSKLAK"
     gene            5811..>6363
                     /locus_tag="VGR03_05365"
     CDS             5811..>6363
                     /locus_tag="VGR03_05365"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV1993739.1"
                     /translation="MKRKLLIAVPLLALVTLVAWMFRPKHETVGEAFVSEKTVSLLSG
                     IAQVREQVGMLHYGERVDVVGKRNDYVKVRTNAGTVGWVESRQLMEPALWQRSIKLLD
                     QTRGMLVQARGRTKVSTNLRVQPGRTEPRLYKFARNVPVEIVGRAVADWVQVTDEKDA
                     ANEPEETKKEDWFLIRGAATRPPG"
BASE COUNT         1407 a         1729 c         1932 g         1295 t
ORIGIN      
        1 gggcacgcaa ttcgtacagg ccgtgggggc tgccgaggcc accctttact acgagggacg
       61 gcccaaggcg ctggcccaag cgaagaaagc gccgctcggt gaattcgtca atcacgagcg
      121 cgaggaaatt gtctatgttt cgagcggcga cggagcgacc agcgagggcg agttttggga
      181 atccctgaac gtcgcgtgcg cgaagaaact acctctgctt tacctggtgg aagacaatgg
      241 ctatgccatc tccgtgccgg ttgaagttca gacggccgga gggagcatct cgaaactggt
      301 gcgagggttt cccgcgctgc acgtggcgga atgcgatggc accgatccca tcgaaagcta
      361 ttccgtctgc aaggaagcgg tgcagtactg ccgcgagcgc cgcggtcccg cgctggtcca
      421 tgcgcatgtg acgcggccgt attcgcattc gctgtccgat gacgaaaaac tctataagtc
      481 cgccgaggaa cgcgaagaag aggcgcgccg cgatccgatt tcaaagtttg ctctttttct
      541 ggtgcgggaa ggcatcctcg atcagaagca gatcgagagc ctggaagcgc aaatcgacaa
      601 tgaagtccgc gacgccgccg accaggcgct ggccgcggaa gagccgtcca tcgattcgat
      661 tcgactcaat gtgtattcgc cggacgtgga tccaacgtcc gccgcgttcg aggcagaacc
      721 ccaattgcac ggtgcgccaa agaccatggt tgagatggtt gcggcgacac tttccgatga
      781 gatggcgcgc gacgagcgca tcagcgtttt cggcgaagac gtcgccgacg ccagccgcga
      841 agagaacctc aggcacgtaa aggggaaggg cggcgtcttc aaagccaccg cgggattgca
      901 gcgcaagtac ggcagcgacc gcgttttcaa taccccgctc gccgaagcca gcatcgtcgg
      961 gcgcgccctg gggatggcca cgcgcggatt gaagcccgtc gcggaaatcc agttctttga
     1021 ctacatctgg ccggcgatga tgcagattcg cgatgagttg tgcgtgatgc gctggcgttc
     1081 gaatggagcc ttcaaggcgc cgatagtcat tcgcgtgccg atcggcgggt atctcacggg
     1141 cggcggggtg tatcacagcc agagcggcga atcgattttc gcgcactgcc cgggcttgcg
     1201 ggtggtgatg ccttccacgg cgctggacgt cgcgggtctt ttgcgcacgg ccattcgctg
     1261 cgacgaccca gtgatgttcc tagaacacaa gcatctttac cgccagccgt acaatcgctc
     1321 ggagaatccc gggcccgatt ttacgattcc gttcggcaag gcccgcatgg tgaaagaggg
     1381 cagcgacctc agcatcatta cctatggcgc agtggtccat cgcgccgaag tggcggcggc
     1441 gcaactggag cgcgagggca tctccgttga aatcatcgac ttgcgttcgc tctcgcccta
     1501 cgactgggaa gccattgcgg cgaccgttcg gaagacgcac cgggtcatcg tggcttacga
     1561 ggatatgttg agctggggtt atggggcgga aatagccgcg cgcattgctg acgaactctt
     1621 cgaggagctg gacgccccgg tcaggcgcgt tgcggccatg gataccttct gcgcgtacca
     1681 gcccaagctg gaggacgtga tcctgccgca gacgagccac attgcggcgg cggtgaagca
     1741 gcttctggag tattgagccg attggcttta gactgccggg tgaggaaact atttcgggtg
     1801 taattgcgta cttatgggta gggatgccag gaaaaatcgc tctgtggggg tgacccaatg
     1861 acttgcgtaa actgccaaaa agacatcgcc gttggttcga ggttttgcta caactgcggc
     1921 accaagcagc cggaaactcc cgcgccaggc ctgtcgccgg agacgggtgg caagaggaaa
     1981 ctgatgcgct cgggaacgga taagaagatt gcgggtgtgt gcgccggcct tgccgactat
     2041 tttgatcttg atcccacgat cgtccgggtc gtgtggttgc tggccatact ctttgccggg
     2101 acgggcgttc ttgcatacct cgtgctttgg attgtgctcc ccgtagctcc ggctggggta
     2161 ataccgacct cggcgactgt ggtttcttag actgccggct aagaccaagc ggacgggtca
     2221 ttccctgacg tttcagtttc cggtttgcgt gcttttactc tcaaatgtgt tgaaaacaaa
     2281 cactttcagc ttatttacgg gcgaagacag cctgactatt tctgtcgaag tttacggcgg
     2341 atatccggcc agaactccct gatgaggtta ttgaggctgg cagcttccag ctgggctgcc
     2401 cagtttacca ccgtattgtg caggctgcgc tcctcgggtg ggtaataaag gttggctaac
     2461 cccgcttcca ttccattccc cgcaatctca gagatgttga aggtattgcc gccggaatcg
     2521 gtcctggtaa cgacgacacg ggataacgcg tacccagctc ggcggaaaaa acctccatag
     2581 ccgagggtat agtaacgtgg gtcttcgtgg gtcaccgcgg ggacaagtgc ttccgtgaaa
     2641 aaagatccag acgcttgatc cgcaaatgcc cgccaataat agcggccata cccagcgagc
     2701 ccgttgtgca actccgggta cgacctcaaa gccatgcttt ggccagcctg cagtcctgac
     2761 caaatgaagg acgaataatc accgctatcc tgtgtcgcaa gcacgaattt cccccgggtg
     2821 gttagcggag caacctctgt gtgggcgctc acggccgcaa aatttggaat gatccaaaac
     2881 atgcgcttgg tctgctggcc ttcggaacta tctatcttcc ggtcgactgc ggttgggagc
     2941 agcgccgcag caggtacggc ggtctcgaat tttgactgag tagcggcgtc cggttgcctt
     3001 gcggaatctt ccgtgcttcc cgattgctgc gtctgctgct cgggccctgg tcccctcttc
     3061 ttgtctggaa ttgctgcctg gggccggacg agtgccggag acagaagaag aattagaacc
     3121 acagcggcat ttctaattat aggcatagcg tactccaatg aaaccccgtg taggcgggaa
     3181 ggagtacatc ggggtacccc catactgctc tcaagcccga agcaatgctg tcctgtacag
     3241 tacaggtcat tgctgtcaaa tagaggaaac catctccgtg gagcttggct tcaaattgct
     3301 tgcgaccgga tagatgttga tagatcccgg tatcaccggc aaccggaaga cacaatagac
     3361 ggctacaagc acaaaaattg cggcaatccc ggaatttcca tgaaaaccag aagcgagagc
     3421 gcaccaagcc ggcgcgatct cgctcagaag aaggtatgaa ttctcgcggt tcgttcgccg
     3481 gtcttacaac ggccaatctc cgcgcaaata aactgcgaaa atgtatacaa ctgaacagac
     3541 acgggtcgtg acaacgtcta gcattcagta cgtgagctta cgcggctaga tccgccatat
     3601 ccatcccagc ccacgtgtat tggaagcgca ggggcagatg aatttgcttt cacggtcctt
     3661 gtatactctg tttcctcctc ggttgccaag gtttgggccc gaggacgtcg ccggtacggc
     3721 agattattac aatcgcctag ctcggcatca tgaggagacg atggatcctc cgggcgtgtg
     3781 gagagggcgc atttcgatgc agctggcgct gagtatacgg ccgttcagga cttcggagaa
     3841 gatacctcaa aagattctgc gccgggtagc aagagtcttg cggagaaacc tgctggaaaa
     3901 tgagtggagg attgtcttgc agaaataagg actgttgtgt tggccagtcc atccccttca
     3961 gttggatatc catggcccac acaagcgttg agggcatcca ccaggacgct actcgcacag
     4021 cttttctgga tagataccct ctcctgaaga tcagttgtcc tccaacgaga gcggccgaaa
     4081 cttttcgatc tgaaaagtaa tgcaacgtga gcccctttct caatgggggc cgccgatgaa
     4141 agtcgtccag tccgagattc ggatgaagct caacgtttgc aaaagcattt ctggctatgg
     4201 aggcgagcga tgaagcgata tttggtaatt gcagtgtgtc tctcggttgc cgcgggcgcg
     4261 gcttacgccg cgactaaaga acaaaagcgc ctcgaaaatt gcggcgttgt cctccaggaa
     4321 atcttgaaca tcccggataa tctgccccag caggttttgg caaaagccga gtgtgtcatc
     4381 gtgttcccat cggtactgaa attggccttc ggaatgggca aagactacgg ccgcggagcg
     4441 atggtttgcc gcacgggcaa ggaattcaat ggaccttggg gtgctcccgc aatgtacgcc
     4501 ctcgaaggcg atagcatcgg aatgcaactt ggtggcgaat ccaccgatct gattctcctg
     4561 gtcatgaacc agaaaggtgc ggcttccatt ctcagcagca agatcaaggt cggtggagac
     4621 gcatccgccg ctgcaggtcc caaagggcgc gatgcttccg caaacaccga tgcctggatg
     4681 cacgcagaga tcctcagcta ttcgcgttcc cgcgggctat ttgccggcat ttccctcgag
     4741 ggttccaccc tgcgcccgga caacaaagca aacgcccgga tctacggccg caaaattaca
     4801 gcaagagcaa tcttgctggg caacaaagtt gccattcccg caacagcccg tcatctcgtt
     4861 cgcgtattgc aaacgaattc accgcggaat gaatcgaagc aggttgcttc aaggtagtca
     4921 atatttcagc ctcctcatgc tctcggggat ccgcggtgag gagtgcgggc cggactccta
     4981 tcgggaacgc ggagatggcc aatcccatct cttttcataa gcaggttgtt cttacgtccc
     5041 gagagagaga tggggcttgg tcaagttccg atagatcggg tcatgggaaa ctgacccctt
     5101 cgaatgattc gcccgacagg gatttacttg gcgagcttcg aatattttgg ggggcgcgac
     5161 ttgcctccac cttcgtagag gggatggtcg agagacaata atcctgcgcc gacggtggct
     5221 agcgcgaagc tgccggccag caccgcgagg gggaattcgt aatcgtgcac cgccagatag
     5281 ccgccggtgc tgtggacctt ccaaatggcc acgcccatct cgatggccag gagcagcgcc
     5341 gccgcccgcg taaacaaccc taagaccaag agaacggcgc caaatacttc caatacgccg
     5401 gaaatgtaaa cgaagtatcc cggcagaccg tgctgcacga agaagccctg catccccgcg
     5461 gcggagtgcg ccagctttgg atagccgtgg gagaagaaaa tcagcccaag agccacgcga
     5521 aggaccagca atcccagcgg ctgaaaggaa ttgagagact tcatggaaaa tcctccgcga
     5581 ttcgttacaa gtgattgatt cgaacgcttg aaggatttta gcagaaaagc aaagccttca
     5641 ccggcgcccg cggcgtggcc ggggtttcag gtacaatctt tagcgaatca cgaagaacaa
     5701 cggggaagca tgacgaaggt tgagacgatt ggtgtcgttg gcggcgtggc gtgtgccgcg
     5761 atcagtgcac tctccatggg cgccgagggc actacgttcg agggcggcgc atgaagagga
     5821 aactcctcat tgccgtgccg ctgctggccc tggtgactct ggtggcgtgg atgttccggc
     5881 ccaagcatga aacggtgggc gaagcctttg tcagcgaaaa aaccgtttcc ctcctgagtg
     5941 gcatcgcgca agtccgcgag caggtgggaa tgctgcatta cggcgagcgc gtggacgtgg
     6001 tgggcaagcg caatgactac gtgaaggtgc gaacgaacgc cggcacggtt ggctgggtgg
     6061 agagccggca attgatggag ccagcgcttt ggcagcgcag catcaagctg ctggatcaaa
     6121 cgcggggaat gctggtgcag gcgcgtgggc gcaccaaggt ctccaccaat ttgcgcgtgc
     6181 agcctgggcg cacggagcca cgcctttata aattcgcgcg aaacgtgccc gtggagattg
     6241 tgggccgcgc ggtggccgat tgggtgcaag tcacggatga gaaagacgca gcgaatgagc
     6301 cggaggaaac gaaaaaagaa gattggtttc tgattcgcgg tgccgcgaca cggccgcctg
     6361 ggg
//
LOCUS       DASYVO010000159         3506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_47732, whole genome shotgun sequence.
ACCESSION   DASYVO010000159 DASYVO010000000
VERSION     DASYVO010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47732"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1366)
                     /locus_tag="VGR03_05370"
     CDS             complement(140..1366)
                     /locus_tag="VGR03_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEV1993740.1"
                     /translation="MSTNAELIITLVKIVLLLFIVLTVNAYLTWFERKVVAHMQSRWG
                     PHRVGPHGLLQPLADGVKFLFKEDPTPAGVDKFVYYLAPLLALALALTSLALIPFGPE
                     PLPWKFFGHDIYLGIAPPDVNIGILALFAITAMGVYGVALAGWSSNSKYPLLGGLRSS
                     AQMISYELALTMSVVGVLLMAGSFNLKKIIEAQAHHPEWGLFGWNVWPQILGFFCFFV
                     AAVAETNRVPFDLPEAESELVAGFHTEYASFKFAMFFIAEYTSMITVSCLCSIMFFGG
                     WLSPFPASWTFTHYLPSAILIPFGLWVIWDGIRYETIFGRILLPGVGTAFAALGAVFV
                     LLPNVNLFIQGPFWLLSKIFAFLFVYVWMRGTLPRFRYDQLMAFGWKLLLPLSIVNVI
                     LTSFAILAKMQWWGAK"
     gene            complement(1375..>3506)
                     /locus_tag="VGR03_05375"
     CDS             complement(1375..>3506)
                     /locus_tag="VGR03_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV1993741.1"
                     /translation="FCYYEGLSLQAACRMCLVEVEKTPKLQVGCTLPVAEGMVVWSDN
                     EKVREARKSMLEFLLTNHPLDCPVCDKGGECELQDMVFRYGADTSRFVEEKIHRPEEK
                     WSELVYYDAPRCILCFRCVRVCDEGMDVKALGVGMRGANSVIIPNRQDHLECEECGMC
                     IDICPVGALTSGTYRYKTRPWEMSYVSTVCTHCSNGCKTTLSVRNHEILRSNNRDLSG
                     INKDFLCVKGRFGFDFIKHAERICQPMVRKGDKLYPVSWEEAAQAAATKLKAVYDASG
                     KDAIGFIGSNRTSNEENYLLQRMARATFGTNNIDHHRTADYTGLVTALGDGTSDSLLT
                     MEQLYQSKAVLLVGNDPTNQNPLAGWQIRSGIRHFGTKLFIINANEIKLKRKATQFVN
                     VAEGQEAGALRWLAHEEGQLSPTLVEQLVQLKAGLEAESDVAVVFGAEVSGAAIAQLV
                     AFGSKLPGKTRYMALGDYANSRGAADMGVLPDRLPGYAYVDNAGAREALEKLWCSVIP
                     SKAGQTAPQMVEAAQAGKLKALYVVGANPLAHFGTLGFGRGKLELLIVHEMFLTETAK
                     VADIVFPAASAYEKEGTVTNTSGEVQMLHKGAEVMGPRSDFDLLRILSHQLEKLGLGK
                     AFHYKTPAAVFEEIRKAVIGYDVQAAGLLTGGAEPTRVQFARNGHAPYDVPVGLIRSA
                     KDTLFTSGTLGRFCTMMESLPEAKTEP"
BASE COUNT          781 a         1172 c          884 g          669 t
ORIGIN      
        1 agcagataaa gtcccgctaa cgcaatcatc accacgatca gcgatagcgc gctatgaatc
       61 ggatgctttt gcaggatcaa actgaccgcg ccgatcaccg caagcgccgc cagcacgaag
      121 aaaacagcga gttgcgcggt catttcgcac cccaccactg catcttcgcc aggatcgcaa
      181 agctggtgag gatcacgttc acgattgaca gcggcagcag caatttccac ccgaacgcca
      241 tcagctggtc gtagcggaaa cgcggcagcg tcccgcgcat ccacacatac acaaacagaa
      301 aagcaaaaat cttggacagc agccagaacg gtccctgaat gaacaggttc acattgggca
      361 acagaacaaa aaccgcgccg agcgccgcga acgcggtccc cacgcctggc agtaggattc
      421 ggccaaaaat cgtttcatag cggatcccat cccagatgac ccacagaccg aacggaatca
      481 ggatcgccga cggcaagtaa tgcgtgaacg tccacgaagc agggaaggga gacagccatc
      541 cgccgaaaaa cataatcgag cacaggcacg aaaccgtgat catgctggtg tattccgcga
      601 tgaagaacat cgcaaacttg aagctggcgt actccgtatg gaagcccgcg acaagctcag
      661 actccgcttc gggcagatcg aatggcacgc gattcgtttc cgcgaccgcc gcaacgaaaa
      721 aacaaaagaa gcccagaatc tgcggccaga cgttccagcc aaaaagcccc cactccggat
      781 ggtgcgcctg tgcctcaatg atctttttca aattgaagct gcctgccatc agcagcacgc
      841 cgaccacgga catcgtcagc gccaattcgt agctgatcat ctgtgccgag ctgcgcagcc
      901 cgccgagcag cggatacttg ctgttcgagg accagcccgc gagcgccacg ccataaacgc
      961 ccatcgccgt gatcgcgaaa agcgccagta tcccgatatt cacatcggga ggcgcgatcc
     1021 ccaaataaat atcgtgcccg aaaaatttcc acggcaatgg ctccggccca aacgggatga
     1081 gcgcgagcga cgtcagcgcc agcgccaaag ccagcagcgg cgcgagatag tagacaaatt
     1141 tatccacgcc cgccggcgtc gggtcctctt tgaacagaaa cttcaccccg tcggccagcg
     1201 gctgcagcaa tccgtgcggc cccacgcggt gcggccccca gcgcgactgc atgtgcgcca
     1261 cgactttccg ttcgaaccac gtcagatacg cgttcaccgt cagaacgatg aacagcaaaa
     1321 gcacgatctt gacgagcgtg ataatcagct ccgcgtttgt ggacaagaaa gaattcacgg
     1381 ctcggtcttg gcctctggca gggattccat catcgtgcag aagcgcccga gggtcccgct
     1441 cgtaaacaaa gtgtccttcg cggatcgaat caatcccacc ggcacgtcgt atggcgcgtg
     1501 tccgtttctg gcaaactgca cccgcgtcgg ctccgcgccg ccggtcagca gcccagcggc
     1561 ctgcacgtca tagccaatca ccgccttgcg aatttcttca aacaccgccg ccggcgtctt
     1621 gtaatgaaac gccttcccca gccccagctt ttccaattga tgcgacaaaa tcctcagcag
     1681 atcgaagtcg ctccgcggcc ccatcacttc cgcgcccttg tgcagcattt gaacttcgcc
     1741 cgatgtgttt gtgactgtgc cttccttttc gtacgccgaa gccgcgggaa aaacgatgtc
     1801 cgccaccttt gccgtttccg tcaggaacat ctcatgcacg atcagcagct cgagtttgcc
     1861 gcgcccgaat ccaagcgtcc cgaaatgtgc cagcggattc gcgcccacca cgtacagcgc
     1921 cttcagcttt ccggcttgcg ctgcttcgac catttgcggc gcggtctggc ccgccttcga
     1981 aggaatcacc gagcaccaaa gcttttcgag cgcttcacgc gcgccggcat tgtcaacata
     2041 cgcgtagccc ggcaaacgat ccggtaacac gcccatatcc gccgcgccgc gcgaattggc
     2101 gtaatcgccg agcgccatgt accgcgtttt gcccggaagc ttcgagccaa aagcaacgag
     2161 ttgcgcgatc gctgccccgg aaacttccgc gccaaacaca accgcaacat cgctctccgc
     2221 ctcaagcccg gccttcaatt gcaccagttg ttcgacgagc gtcggcgaca gttgaccttc
     2281 ctcatgcgcc agccaccgca gcgcgcccgc ctcctggccc tccgccacgt ttacaaactg
     2341 cgtggccttg cgcttcagtt tgatttcgtt cgcattgatg atgaatagct tcgttccaaa
     2401 gtggcgaatg ccgctgcgaa tctgccaccc ggccagcggg ttctgattcg tagggtcgtt
     2461 ccccaccagc agaaccgcct tcgactgata caactgctcc atagtgagca gggaatcgct
     2521 tgtaccgtcg cccagcgccg tcaccaatcc cgtgtaatcc gccgtgcggt ggtgatcgat
     2581 gttgttcgtt ccaaaagtcg cgcgcgccat ccgctgcaac aaataattct cttcattcga
     2641 agtgcgattc gaaccgatga agccaatcgc atccttgccg ctcgcgtcgt agaccgcctt
     2701 caacttcgtc gccgcagcct gcgcggcttc ttcccacgac acgggataaa gcttgtcccc
     2761 cttgcgaacc atcggttggc aaatgcgctc cgcgtgcttg atgaagtcaa atccaaaccg
     2821 ccccttcacg cacagaaaat ccttgttgat tccgctcagg tcccggttgt tggaacgcag
     2881 aatctcgtgg ttgcgcacgc tcagtgtcgt cttgcagccg ttcgaacagt gcgtgcacac
     2941 cgtcgagacg tacgacatct cccacggccg cgttttatac cggtacgtcc cgctcgtcag
     3001 cgctcccacc gggcaaatat cgatgcacat cccgcactct tcgcactcca aatgatcctg
     3061 gcggttcgga atgatcacgc tgttcgcccc gcgcatcccc acgcccagcg ccttcacgtc
     3121 cattccttcg tcgcacacgc gcacgcaccg gaagcacagg atgcaccgcg gcgcatcgta
     3181 ataaaccagt tctgaccatt tttcttccgg ccggtgaatt ttttcctcaa caaacctgct
     3241 cgtgtccgcg ccgtaacgga acaccatgtc ctgcaattcg cactcgccgc ccttatcgca
     3301 caccggacaa tccagcgggt ggttggtgag caggaactcc agcatggatt tgcgcgcttc
     3361 gcggactttt tcgttgtcgc tccacaccac catgccttcg gccacgggca gcgtgcagcc
     3421 tacttgcagc ttgggagtct tctcgacttc caccagacac atgcggcagg cagcttgcag
     3481 gctaagccct tcgtagtagc agaagg
//
LOCUS       DASYVO010000160         4751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_132245, whole genome shotgun sequence.
ACCESSION   DASYVO010000160 DASYVO010000000
VERSION     DASYVO010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132245"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VGR03_05380"
     CDS             complement(<1..394)
                     /locus_tag="VGR03_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993742.1"
                     /translation="MNQPDSIAVAAILIGFGLTTIMFRVQRELYVREVKKEENWIAWA
                     DFLIFASVLLVLLLIVVPLFAFSVQTFAPAASAAAFILQAGYIPAILAHYRIGIGKGR
                     NVKGLVYKRGEPAEKWIVCVSGAIALLFF"
     gene            complement(684..1160)
                     /locus_tag="VGR03_05385"
     CDS             complement(684..1160)
                     /locus_tag="VGR03_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993743.1"
                     /translation="MTLARAAVQKRGLDYFRHVVGLTYREIGALQARNHQVIFSKANI
                     AGRFFGQNRKAAARALYKSSLSDTSITQTLGRYAKDTGLTLEDVYEAFRDGNWSLAPG
                     VCAFGGPKWAAIANVAIQLGAAIQGQIWPEVARLADLLDTLEHNKANIIDKFAQLD"
     gene            complement(1157..1561)
                     /locus_tag="VGR03_05390"
     CDS             complement(1157..1561)
                     /locus_tag="VGR03_05390"
                     /inference="COORDINATES: protein motif:HMM:NF022963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HEV1993744.1"
                     /translation="MASHASGTFEVKLVPQPPEDKAEESTLARMSIDKQFYGDLDGTS
                     KGQMLTAGTDVKGSAGYVAIERITGTLQGRTGSFVLQHTGTMNRGASQLSITVVPDSA
                     TGQLLGLTGKMTITIADGKHSYEFDYSLPEAR"
     gene            complement(1893..3338)
                     /locus_tag="VGR03_05395"
     CDS             complement(1893..3338)
                     /locus_tag="VGR03_05395"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV1993745.1"
                     /translation="MSSPCTNLVPALEAILGASRVITDPAARATYAVDDFIPSVIAKP
                     VSPEEVAEIVRFAALEKLSLIPCGARTKLRIGMPPSRYDIALDMTSLDQIVHYDPGDL
                     TLSAGAGTPIAKLNATLLEHHQFLPLLVPWYSLSTIGGAIASGIDSPLRQFYGTARDF
                     LIGAEFVTGSGAITKSGGRVVKNVTGYDLHKLLIGSLGTLAVITRLNFRTFPAPQAGS
                     CGFVASFPNAEGTLALRRAIAQSPLTPLTFDILSPELAQIFATRTPATPEAAVFAGEN
                     RSADHTSLPPIGDWFHPREWQHCAAFAGTPEVLGRYTRDLTRLAEQSRASSTTILDDT
                     TRPAVWGRLRETISMLLECSPATTIFKLSVLPSHHAALFAKLKQIADHAGLPHALVAR
                     AGGTIYFALFPASTNDETTRRLARAAAQIFQSVSVEGGDASLLVAAPTLKVRVNIGGP
                     TRPDSALMRRLKSAFDPQNIFAPGRLVFGLP"
     gene            complement(3465..>4751)
                     /locus_tag="VGR03_05400"
     CDS             complement(3465..>4751)
                     /locus_tag="VGR03_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEV1993746.1"
                     /translation="QVSRIVKLAAEHHLPIVGRGAGTGLSGGALARTGGVMLVFARMN
                     RILELDIENQRAVVQPGVVNYELTRAAEPHGLYFAPDPSSWKSCTIGGNVAENAGGPH
                     TLAYGVTTNHVTALELVLPSGEIVRVGSKHGDAPGYDLTGLFVGSEGTLALVTEITVK
                     LSRKPEAVKTLLAIFDSVDDAAETVADITARAITPAACEMLDGWTLRAVEDYIHAGFP
                     MDSAAVLLIEVEGLTETVAAQAAAITEVCSAHHAREVRLARDNAERDLLWKGRKNAFG
                     ALGRLAPSNYVLDGVIPRSKLPQALRRIRQIGDQFGFQIGNIFHAGDGNLHPIVLYDP
                     RDTAQFQRALEASAEIIRYCVEVGGALTGEHGVGMEKSELMPLLFSDADFDLMRRVHD
                     AFNPDSALNPGKIFPLSKGCGEIRVRPKSPHPASAS"
BASE COUNT          956 a         1133 c         1700 g          962 t
ORIGIN      
        1 taaaaaataa cagcgcaata gcacctgaca cgcacacaat ccacttttct gctggttctc
       61 ctcttttata gacgaggcct ttcacatttc tgcccttgcc gataccgatt cggtagtgag
      121 ctaagattgc ggggatatac ccggcttgca gaatgaatgc ggcagcagaa gctgcaggtg
      181 caaatgtctg gacggagaag gcaaagaggg ggacaacgat cagaagcaac accaacagca
      241 ctgaggcgaa gattagaaaa tcagcccagg caatccagtt ttcctccttc ttcacctctc
      301 gcacatagag ttcccgctga acgcggaaca tgatcgtggt tagcccaaag ccaataagga
      361 tggcagctac cgctatagag tcaggttgat tcaacgaggt ggcctcatta tttcccttga
      421 ggaattgctg gggcgtcctg ctccttgtcg attgttactt gcgagaacat aggaagtcaa
      481 tgaaaaatgt tggggacgaa tgggcccggc gtaatttctg ggtctgttgt cacgactaaa
      541 aaataccgat tagtagtgcg gttggttcac catgtagttg acaggaatca tctgagccct
      601 gacgccttac gtttcagcca cggcagctca tcatcgcacc aaccgccctt tgctgcagag
      661 gctcgttagc tagcgtgtcg gcgctagtcg agctgagcaa atttatcgat gatattggct
      721 ttgttatgct cgagcgtgtc tagcaaatca gctagccttg caacttcggg ccaaatctgg
      781 ccttggattg ccgccccgag ctgtattgca acgtttgcaa tcgcggccca ttttggtccg
      841 ccgaacgcac agactccggg cgcaagactc cagtttccgt cgcgaaaagc ttcgtagacg
      901 tcttcgagag tcagcccagt gtcctttgcg taccggccga gagtctgagt gattgaagtg
      961 tccgagagac tgctcttgta gagcgctctt gcggctgcct tgcggttttg accgaagaat
     1021 ctcccggcaa tattggcttt ggagaagatg acttggtggt tgcgcgcctg gagagctccg
     1081 atttctctgt aagttagccc gaccacgtgc ctgaagtaat cgagacctcg tttttgaacg
     1141 gcggcacgag cgagtgtcat cgggcttctg ggagggagta gtcgaattcg taggaatgct
     1201 tgccgtcggc gatggtgatg gtcattttgc cggtgaggcc gagcaactgg ccggtggcgg
     1261 aatccgggac gacggtgatg gaaagctgag aagcgccgcg gttcatggtg ccggtgtgct
     1321 ggagcacgaa gctgccggtg cgaccctgaa gcgtgccggt gatgcgctcg atggcgacgt
     1381 agccggcgga gcctttgacg tccgtgccag ccgtcagcat ctggccctta ctcgtgccgt
     1441 cgagatcgcc gtaaaattgt ttgtcgatgg acatcctggc gagggttgac tcctcggctt
     1501 tgtcttccgg cggctgaggg accaacttta cttcaaacgt tccgctcgca tgtgaggcca
     1561 tggctgttcc cttctggact gggtcttgaa ctgggagatg tgtgcgtgta aacgccgcag
     1621 agccaaagcc aagggataag cagatggcgg tgatgacacc gactccggcg cgagatcgct
     1681 tgttcatact tgctccgtca tgcccgcgat ggcttcggtg gccggcagtg gaagaaggca
     1741 cggccaccaa atcagaacga cttgttaaag catagggaac tcccgctatg cattcaagag
     1801 ctatgccaag aactgcccgc tgatcggcgc agaggtagaa atagagtctc gacgccgacg
     1861 atggtgcagc ggtccattct cttcgacggc ggtcaaggta ggccgaatac gaggcggccg
     1921 ggggcgaaga tgttttgcgg atcgaatgcc gatttgaggc ggcgcatgag agccgagtcg
     1981 gggcgcgtgg ggcccccaat gttgacgcgt acttttagtg tgggcgcggc gacgaggaga
     2041 ctggcgtcgc caccctccac agagaccgat tggaagattt gggctgcggc tcgggcgaga
     2101 cgtctggtgg tttcgtcgtt tgtggatgcg gggaacagtg cgaagtaaat cgtaccgccg
     2161 gcgcgggcta cgagtgcgtg ggggagtccg gcgtgatcgg cgatttgctt caactttgcg
     2221 aagagggcgg cgtggtgcga ggggaggacg ctaagtttga agatggtggt ggcgggcgag
     2281 cactcgagca gcatggaaat ggtttcgcgg aggcggcccc agacggcggg gcgcgtggtg
     2341 tcatcgagaa tggtggtgga actagcgcga gattgctcgg ctaggcgagt gaggtcgcgc
     2401 gtgtaacgac cgaggacttc gggagtaccg gcgaaagcgg cgcaatgttg ccactcgcgc
     2461 ggatgaaacc agtcgccgat gggaggaagc gaagtgtggt ccgcgctgcg gttttctccg
     2521 gcgaagacgg cggcttccgg ggtggccgga gttcgagtgg caaagatttg ggctagttcg
     2581 ggactgagaa tatcaaacgt gagcggagtg agtggagatt gcgcgatggc tcggcgaaga
     2641 gcgagcgtgc cttctgcgtt gggaaaggag gcgacgaaac cgcagctgcc tgcttgggga
     2701 gccggaaatg tgcggaagtt gaggcgggtg atgacggcga gggtgcccag cgagccgatg
     2761 aggagtttgt ggaggtcgta gccggtgacg tttttgacaa cccggccgcc gctttttgtt
     2821 attgcgccag agccggtgac gaattctgcg ccgatgagga agtcgcgcgc agtgccgtag
     2881 aattggcgca agggggagtc gatgccggag gcgatggcgc cgccgatggt gctgagggaa
     2941 taccagggaa cgaggagcgg aaggaactga tggtgttcga gcagggtggc gttcaatttt
     3001 gcgatgggcg tgccggcgcc ggcgctgagg gtgaggtcgc cgggatcgta gtgcacgatt
     3061 tggtcgaggc tggtcatgtc gagagcaatg tcgtagcggg aaggaggcat gccgatgcgg
     3121 agtttggtgc gcgcgccgca ggggataagg gagagttttt cgagcgcggc gaagcggacg
     3181 atttcggcga cttcttcggg ggagactggt tttgcgatga cggaggggat gaaatcgtcg
     3241 acggcatagg tggcgcgggc ggcgggatcg gtgatgactc gcgaagcgcc taggatagct
     3301 tccagtgcgg ggacgagatt ggtgcaagga ctagacatga ttgctggtga agcgctgaac
     3361 aaggatgggg gcgacgaaag ttgaaagtcg aaagaaaaag aaggaattaa cgcagagggc
     3421 ccagtgagcg caggggccgc agagaagaga agcggggaga aagctcacga ggcgctcgca
     3481 ggatgcgggg acttggggcg gacgcggatt tcgccgcagc ctttgctgag gggaaagatt
     3541 ttgccgggat tgagagcgga atcgggattg aaggcgtcgt ggacgcggcg catgagatcg
     3601 aagtcggcgt cggagaagag gagcggcatg agctcggatt tttccatgcc gacgccgtgc
     3661 tcgccggtga gcgcgccgcc gacttcgacg caatagcgga tgatttcggc ggaggcttcg
     3721 agggcgcgct ggaattgcgc ggtgtcgcgc ggatcgtaaa gcacgatggg atgcaggttg
     3781 ccgtcgccgg cgtggaaaat gttgccgatt tggaaaccga attgatcgcc gatttggcgg
     3841 atgcggcgga gagcctgcgg gagtttcgaa cgaggaatga cgccgtcgag cacgtaattg
     3901 ctgggggcca ggcggccgag tgcgccgaaa gcgtttttgc ggcccttcca gaggaggtcg
     3961 cgctcggcgt tgtcgcgcgc gaggcggact tctcgggcgt gatgcgcgct gcagacttcg
     4021 gtgatcgcgg cggcctgagc ggcaacggtt tcggtgaggc cttcgacttc gataaggaga
     4081 acggcggcgc tatccatggg gaaaccggcg tgaatgtagt cttcgacggc gcggagagtc
     4141 cagccatcaa gcatttcgca ggcggccgga gtgatggcgc gggcggtgat gtcggcgacg
     4201 gtttcggcgg catcgtctac ggaatcgaaa atggcgagaa gtgttttcac ggcttcgggc
     4261 ttgcgcgaaa gctttacggt gatttcggtg accagggcga gcgtgccttc cgagccgacg
     4321 aagaggccgg tgagatcgta gccaggcgcg tcgccgtgct tgctaccgac gcgcacgatt
     4381 tcgccactgg gaagaacgag ttcgagggcg gtgacgtgat tggtggtgac gccgtaggct
     4441 agggtgtgcg ggccgccggc gttttcggcg acgttgccgc cgatagtgca ggatttccaa
     4501 ctggaaggat cgggggcgaa gtagaggccg tgcggttcgg cggcgcgggt gagttcgtaa
     4561 ttcactacac caggttggac gacggcgcgc tgattttcga tgtcgagttc gagaatgcga
     4621 ttcatgcgcg cgaagacgag catcacgccg ccggtgcggg cgagcgcgcc tccggaaagt
     4681 cccgtgccgg cgccgcggcc gacgatgggg agatggtgtt ccgcggcgag tttgacgatg
     4741 cgggagactt g
//
LOCUS       DASYVO010000161         2458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_271951, whole genome shotgun sequence.
ACCESSION   DASYVO010000161 DASYVO010000000
VERSION     DASYVO010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271951"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VGR03_05405"
     CDS             complement(<1..867)
                     /locus_tag="VGR03_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993747.1"
                     /translation="MNAQKSIAWVLWLVLPVGFAGIWRLQHSIDAQRASLSQERDDVL
                     LRSGKLVKLMSLEYAPLLADIYWTRVVQYYGNKHVRGQANLELLWPLLDITTTLDPNL
                     LISYRFGAMFLSQAAPTGAGRPDLAVQLIQRGIQANPDYWRLYEDLGFVYYFDLKDYQ
                     KASEAFLEGSKKPTAQLWMKVMAARVAAEGESFETSAFLWKDIYESTADPSVKKNAQL
                     HLQLLRVREDCKQLDALADEFAKRYGKRPARMSEMVQAGLLRGIPGDPLGFAYVFGKD
                     GKAELNLDSPLLK"
     gene            complement(864..1640)
                     /locus_tag="VGR03_05410"
     CDS             complement(864..1640)
                     /locus_tag="VGR03_05410"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1993748.1"
                     /translation="MRRAGVVALNTFREAVRDRVLYNLVFFALLMMAAAIAVGQISIG
                     IEQTVVVSLGLSAISVIGLLISVFLGVALVSKEMDKRTLYALLAKPVRRWEFLLGKFA
                     GLVLTLAVNTAAMALGLLLVMLYVKHSLERSDAVVLVAVYFVLLKLALIVALALLFSC
                     FTTPLLAILFTVGLYIVGLYVQELRNLPVEVMTPAMAAFTKWLSYLLPNFENFNVMAM
                     AAHGRAVPGALILQNTLYTVVYCTIVLIASAAVFSRRNLK"
     gene            complement(1637..>2458)
                     /locus_tag="VGR03_05415"
     CDS             complement(1637..>2458)
                     /locus_tag="VGR03_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1993749.1"
                     /translation="GPNGAGKTTTIKLLMALIFPTAGSARILGKPISDVEMHRDIGYL
                     PEQPYFYDYLTAAEVLDYFARFHDLTAADRKDRVERMLKKVGLETARKIQLRKYSKGM
                     LQRVGLAQAILHDPKVVILDEPMSGLDPVGRREVRDIILELKRAGKTVMFSTHILSDA
                     EMLCDRVGVIVGGKLRGVGAPGTLVDMKTQGMEILFEFTGTSNAPLLSKATRTGDCYR
                     LQVAEEELYAAIDQLRGAGARILAVSQVKATLEEYFMHLVEVDRAQASAVEVSGK"
BASE COUNT          526 a          818 c          614 g          500 t
ORIGIN      
        1 tttaaggagc gggctatcca gattgagttc cgcttttccg tccttgccga aaacgtaggc
       61 gaaacccagg ggatcgcccg gaattcccct taacagcccg gcctggacca tctcgctcat
      121 ccgcgccgga cgtttaccat accgcttcgc gaattcatcg gccagcgcat cgagctgttt
      181 gcagtcttct ctcactctta gcaactgcag atgcagctgc gcattcttct tgaccgaggg
      241 atcggccgtc gattcgtaaa tgtccttcca caaaaaggcg gaggtctcga aggactcccc
      301 ttccgcggca acccttgcag ccattacttt catccacagc tgcgcggttg gtttcttgct
      361 tccttccaga aaggcttccg aagccttttg ataatctttc agatcgaagt agtagacgaa
      421 tcccagatct tcatacaagc gccagtaatc cggattcgct tggatgccac gctggatcag
      481 ttgcaccgcc agatccggcc tgcccgcccc ggtaggcgcc gcctgactga gaaacatcgc
      541 gccgaaccgg tacgaaatca gcagattcgg atcgagcgtc gtggtgatat ccaggagcgg
      601 ccagagcaat tcgagattcg cctggcctcg tacgtgtttg tttccgtagt actgcaccac
      661 gcgcgtccag taaatgtccg ccagcagcgg cgcatattcc aggctcatca atttcacgag
      721 ttttcccgag cgcagcagga cgtcgtcgcg ctcctggctt aacgaagcgc gctgcgcgtc
      781 gatgctgtgc tgcagccgcc agatgcctgc aaatcccacc ggcagcacca gccacaacac
      841 ccacgcgatg gatttctgcg cgttcatttc agatttcggc gcgaaaacac cgcagccgag
      901 gcaatcagaa caatcgtgca ataaaccacc gtgtacagcg tgttttgcag gatcaatgcg
      961 ccaggaaccg ctcgcccgtg cgcggccatg gccattacgt tgaaattctc aaaattcgga
     1021 agcagatagg aaagccattt ggtgaaggcg gccatcgccg gagtcatcac ctcgaccggc
     1081 aaattccgca gctcctgcac gtacaaaccc acgatgtata agcccaccgt gaacaaaatc
     1141 gccagcagcg gcgtcgtaaa gcaggaaaac agcagcgcca gcgcgacgat cagcgccagc
     1201 ttcagcagta cgaaatacac cgccaccagc actaccgcgt cgcttcgctc caatgaatgc
     1261 ttcacgtaga gcatcaccag cagcagtccc agggccatcg ccgccgtgtt caccgccagc
     1321 gtcagcacca gccccgcgaa ttttccgagc aggaattccc atcgccgcac cggcttcgcc
     1381 agcagcgcgt agagcgtgcg cttgtccatt tccttggaga ccagcgcgac gccaaggaaa
     1441 accgaaatca gcagcccgat cactgaaatg gcactcaagc ccagactcac gacgaccgtt
     1501 tgctcgatcc cgatcgaaat ctgtcccacc gcgatggccg ccgccatcat cagcagcgcg
     1561 aaaaacacca ggttgtagag cacgcggtcg cgcaccgctt cgcgaaacgt attcaatgcc
     1621 actacgccgg cgcgcctcat ttcccgctca cttccaccgc cgatgcctgc gcgcggtcca
     1681 cctccaccag gtgcatgaag tattcttcca gcgtcgcttt cacttgcgac accgcgagga
     1741 tgcgcgcccc agctcctcgc agttgatcga tcgccgcata cagttcttcc tcagccacct
     1801 gtagccggta acaatcgccc gtgcgtgtcg ccttggacag cagcggtgca ttgcttgtcc
     1861 ccgtgaactc aaaaagaatc tccattccct gcgttttcat gtccacgaga gtgcccggtg
     1921 cgccgactcc gcgcagcttt cctccgacaa tcacgcccac gcggtcgcat aacatttccg
     1981 cgtccgagag aatgtgcgtg gaaaacatca ccgtctttcc agccctcttt aactccagaa
     2041 taatgtcgcg cacttcgcgc ctgccgaccg ggtccagccc tgacatcggc tcatccagaa
     2101 tcactacttt cggatcgtgc agaatagctt gcgccagccc cacgcgctgc agcatcccct
     2161 tcgaatactt gcgcagttgt atcttgcgcg cggtttccaa ccccaccttc ttcagcatgc
     2221 gttccacgcg atccttccgg tcagccgcgg tcagatcgtg gaaccgcgcg aaatagtcga
     2281 gtacttccgc cgcggtcaaa taatcgtaga agtagggctg ctctggcaga taaccgatgt
     2341 cccggtgcat ttccacgtcg ctgatcggct tgccgagaat tcgcgcgctc cccgccgtgg
     2401 ggaaaatcag cgccatcagg agcttgatgg tcgtcgtttt accggcgccg ttcggccc
//
LOCUS       DASYVO010000162         3549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_271954, whole genome shotgun sequence.
ACCESSION   DASYVO010000162 DASYVO010000000
VERSION     DASYVO010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271954"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..1425)
                     /locus_tag="VGR03_05420"
     CDS             complement(262..1425)
                     /locus_tag="VGR03_05420"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1993750.1"
                     /translation="MKAAVRHQLDPTIRSNDTCDFHSSLRAKIVGQEEGVQSLVDMFQ
                     VFCAGLNSPGRPVGNLLFLGPTGSGKTRIVEAAAEILFGDARAVIKVDCAEFQHSHEI
                     AKLIGSPPGYLGHRETHPLITQEALAASYTEKLKLSFLLFDEIEKASDALWQLLLGML
                     DKATLTLGDNRRVDLSQTVIFLTSNLGGGEITELMQGGMGFVRPKDQPAAGFDEKIER
                     TAVEAARRKFSPEFMNRLDKVVVFHPLRREQLERVLEIELGQVQQRVLETAKGQFLFR
                     VTDTGRDFLLQEGTDQRYGARHLKRSIERHVVYPLANLLATEQVHLGDLVCIDWNKEQ
                     DRLTFVREGENLAIPTRKTEPVISARTAPTKTGKSADAPGVTLSPEPTPRMAR"
     gene            complement(1718..2935)
                     /locus_tag="VGR03_05425"
     CDS             complement(1718..2935)
                     /locus_tag="VGR03_05425"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1993751.1"
                     /translation="MSDYILHLDPSKRSSDTIDFETALRSKIVGQEEGVHALVDLYQV
                     FRAGLNSPGRPVGNLLFLGPTGSGKTRIVEAAAEILFGEARAVIKIDCAEFQHSHEIA
                     KLIGSPPGYLGHRETHPLITQETLGASHTDKLKLSFLLFDEVEKASDALWQLLLGILD
                     KATLTLGDNRKVDLSQTVIFLTSNLGGSEIAELMQGGMGFIQPTDKPIAGLDEKVERT
                     AVEAARRKFSPEFMNRLDKVVVFRPLKREQLQEVLDIELGEVQQRVLDTAKGQFLFRV
                     TDAGRDFLLQEGTDQRYGARHLKRAIERHVVYPLANLLATDQVNLGDLVRIDWDEKHK
                     QLTFVREGEGALVQSASREAVGAAAAEAKDGKSAETPTEAAVEERKSRAAQPGGGPAA
                     SPQGRKKQDRSNT"
     gene            complement(2932..3384)
                     /locus_tag="VGR03_05430"
     CDS             complement(2932..3384)
                     /locus_tag="VGR03_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993752.1"
                     /translation="MAFVRRKGNSFYLVHNVRRGGKVRQLHLARLGARARITDDLVRE
                     VSRKHPFVELNWKALREQLNGQVDLANPNSPAVQKLVSSLRALTLDLADVFPPLLRIS
                     ESPVVARELLVQLRLLQSTIQVKLDQFGRGRGRFSNANPLRGLARRRS"
BASE COUNT          738 a         1079 c          985 g          747 t
ORIGIN      
        1 agtcaaaagc aaggacaggc ccaggaagaa cccgaccgcc aatccgcgat gcgctgctct
       61 ggacgcccag agcgcttgac taccgttccc tctgaaaatt tcccatttcc agctgcgggt
      121 gctcacgtca acctccgaaa agacctgtga agaaggatgg cactcctccg ccaaaggtta
      181 ctttagcacc cgcgtgacaa caaaagaaaa agcccgggcg gtcgtccacc cgggcaggat
      241 tagagagaga acaaaattga cttaacgagc catgcgcggc gtgggctcag gcgaaagtgt
      301 cacgccgggc gcatccgcag acttgccggt ctttgtggga gcggtgcgcg cggaaatgac
      361 gggctccgtc ttgcgtgttg ggatcgccag gttttcaccc tcgcggacga aggtcagacg
      421 gtcctgctcc ttgttccaat cgatgcaaac gaggtcgccg aggtgaacct gttcggttgc
      481 caataaattc gccagtggat acacgacgtg gcgttcgatg gagcgcttga ggtgccgtgc
      541 gccgtagcgt tggtccgtgc cttcctgcag gaggaagtcg cggcccgtat cggtcacgcg
      601 gaacaggaac tgccccttgg cggtttccag aacgcgctgc tgcacctggc ccagctcgat
      661 ttccaggacg cgctcaagct gctcgcgcct cagcggatgg aacacgacca ctttgtcgag
      721 gcgattcatg aattccggtg aaaacttgcg ccgggctgct tccacggccg tacgttcgat
      781 cttttcatcg aagccggcag caggctggtc cttcggccga acaaagccca tgccgccctg
      841 catgagttcg gtaatctccc cgccgccgag gttcgacgtc aggaaaatga cagtctgaga
      901 gaggtccacg cggcggttat cgcccagcgt gagcgtggcc ttgtccagca tcccaagaag
      961 caattgccac agggcatcgc tggccttctc gatctcgtcg aagagcagaa agctgagctt
     1021 tagcttttct gtatagctcg ccgccaacgc ttcctgagtg attaatggat gggtctcgcg
     1081 gtggcccaaa tagcccggag gtgagccaat cagcttggca atttcgtgcg agtgctggaa
     1141 ctccgcgcaa tctaccttga tcaccgcgcg agcatccccg aataggatct ccgccgctgc
     1201 ttccacaatg cgcgtctttc ccgaaccagt cggacccagg aacagcaggt tcccaaccgg
     1261 acgcccgggc gagttcaacc cagcgcaaaa aacctgaaac atgtccacaa gggactgcac
     1321 gccctcttcc tgcccaacga tttttgcgcg caaggaagag tgaaaatcac atgtgtcatt
     1381 gctgcgtatg gttggatcca gttgatggcg aacggcagct ttcatttcat caacccttct
     1441 tcgcgtctag ctcccccaaa cgcgctgcgc gtctcagctt cgcgcttccc tgagatagtg
     1501 catggggagt gccaatcttt tccgcagctt cgaggatcga aaagcagcgc aaactcaaca
     1561 acatacagga tttgcagttc tggaaattgt ctcaacccgt gagacagtgt ttcatcggcg
     1621 tgcaaagtga gagcggtgaa acagtttgtg tcagtggcca gacgacaaaa aggggcgcac
     1681 ctttcgacgc gcccccggcc aaagttaaag tgctgcttta agtatttgag cggtcctgtt
     1741 tcttgcgtcc ttgcggcgac gctgcaggtc ccccgccggg ctgtgccgcc cgcgatttcc
     1801 tctcttcaac cgcggcttcc gtcggtgtct cggcggattt cccgtccttc gcctcggcag
     1861 ccgcagcgcc gacggcctcc cgtgaggcgg actgtacgag ggcaccctcg ccttcgcgca
     1921 cgaaggtgag ctgcttgtgc ttctcatccc aatcgatccg taccaagtcg cccaagttga
     1981 cttgatccgt ggccaacaga ttcgccaacg gataaacaac atgccgctcg atggcgcgtt
     2041 tcaggtgccg agcgccatag cgctggtccg tgccttcctg cagaaggaaa tcgcggccgg
     2101 catcggtcac ccggaataga aactgcccct tggcagtgtc caggacgcgc tgctgcacct
     2161 cacccagctc gatatcgagc acttcctgga gttgttctct cttcagcggg cggaagacca
     2221 ccaccttgtc gagccggttc atgaactccg gcgagaactt acgccgcgcc gcttccacgg
     2281 ctgtgcgttc caccttctcg tcaagacctg cgatcggctt atctgtcggc tgaatgaagc
     2341 ccatgccacc ttgcatgagc tcggctattt cactgccacc caggttggac gtcaggaaga
     2401 tcacggtttg cgacaaatcc acttttcggt tgtcccccag ggttagagtg gccttatcaa
     2461 gtatcccgag gaggagttgc cagagtgcgt cactggcctt ttcaacttcg tcgaagagca
     2521 agaaactgag cttcagcttg tccgtatggc tggcacccag cgtttcctgc gtaatcagcg
     2581 gatgagtctc gcgatgcccc agataccctg ggggcgagcc gatcagcttg gcaatttcat
     2641 gggagtgctg aaattccgcg caatcgatct tgatcaccgc acgggcctcg ccaaacagaa
     2701 tttcggcggc cgcttccacg atgcgggtct tccccgaacc cgtcggcccg agaaacagta
     2761 gattccctac cggtcggcct ggcgaattca atccggcgcg aaagacctga tacaggtcca
     2821 ccagcgcatg gacgccctct tcctgtccga caatttttga ccgcagcgcg gtctcgaaat
     2881 cgatcgtatc cgagctgcgc ttgctgggat cgagatgaag gatgtagtcg ctcatgaccg
     2941 cctcctagcg agcccgcgaa gcgggttcgc attgctgaat cgcccgcgtc cacggccaaa
     3001 ctggtcgagc ttgacctgaa tggttgactg caggagccgc agttgcacaa gcagctcccg
     3061 tgccaccacg ggtgattcag aaatccggag gagcggcgga aaaacgtccg caaggtcgag
     3121 agtgagcgct cgaagagagg aaaccagctt ttggaccgcg ggcgagttag gattcgccaa
     3181 gtccacttga ccgttcaatt gttcgcgcag tgctttccag ttcagctcca cgaagggatg
     3241 cttcctggaa acttcacgca ccaggtcatc ggtaattcgc gcgcgggctc cgagacgggc
     3301 cagatggagc tgccgcactt ttccgccgcg gcgcacgttg tgcaccaggt agaacgaatt
     3361 tcctttgcgg cggacaaaag ccacgacgtc acctcaaact gccgtcatcc cgcgtagtgt
     3421 aattctgttc cactacgctc tacaacatta gacgcatttt ttcgccaaga gttgcatcca
     3481 atcagtaacg cgtccaacgt agtggaaacg ccgcgcaaac tctcatcact ttttttgctg
     3541 cgtttcatc
//
LOCUS       DASYVO010000163         7088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_32750, whole genome shotgun sequence.
ACCESSION   DASYVO010000163 DASYVO010000000
VERSION     DASYVO010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32750"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VGR03_05435"
     CDS             <1..967
                     /locus_tag="VGR03_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1993753.1"
                     /translation="YYPKNSLKARLCVEGNRLTYEFCAKHKVPFRHCGKLVVAVDARE
                     EADLIALKKRGEENGVAGLRLIGADEIRAREPHIKGFAALDVPSTGIVSAEELVHAYA
                     RVATNQGANIVTRAQVVSLEPSGKSVRVGLRIGDEEDAQNETIEARCVINAAGLYADE
                     VAAMLGNHSWKIYPVRGEYCEVRGPRSALINNLVYPLPHSDGLSLGVHFTKTLWGTFL
                     LGPTATYVEGKDNYEKNRLPIADFAHSAKTLLPEIEESDLQLGYSGLRPKLVPPDHKG
                     IVDFVITRDTAVPQAIHLVGMESPGLTAAPSVAEHVAQLVAETFE"
     gene            complement(1023..2285)
                     /gene="tyrS"
                     /locus_tag="VGR03_05440"
     CDS             complement(1023..2285)
                     /gene="tyrS"
                     /locus_tag="VGR03_05440"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV1993754.1"
                     /translation="MTQSPFKPVEEQLAYLKKGVAEIVPEAELTAKLEKSRMTGKPLR
                     VYLGVDPTAPDIHLGHTVVLRKLKHFQDLGHTAIFLIGDFSAMIGDPTGQSETRPPLS
                     REQVNANAKTYLAQVFNLLDPKKTEVRYNSEWLDKLSSYEVVRLCGHYRLARMLERED
                     FRSRLANNQPISVHELLYPLLTAYDAVALQSDVEIGATEQKFNLLVHRDIQREYGLAS
                     EVALTMPILVGLDGHRKMSKSLGNYIGITEPPSEMFGKLMSIPDSAMWSYYDAVTDRT
                     PDEIAALKKQTSSGAAHPMDVKMDLALEVTSGFHGRISAQMAKEEFERVFRRRESPED
                     APVVNVPLYGWKPDLIPGTTRIKAVKFFAKLTHSSRSEMERLIKQGAVQIDDSRLTDV
                     SAEIDLGTPRIFLLRVGKFTTLRVNVTP"
     gene            complement(2362..2964)
                     /gene="purN"
                     /locus_tag="VGR03_05445"
     CDS             complement(2362..2964)
                     /gene="purN"
                     /locus_tag="VGR03_05445"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV1993755.1"
                     /translation="MKKRIGVLLSGRGSNFEALAESVAAGRIPNAEIAIVLSNREGAP
                     GIQRAEARGIKTRVIPSKGLEREVYDRHVAAVLDEQKVDLICLAGYMRLLSPFFVAKY
                     LNRILNIHPSLLPSFPGLESQRQALEYGVKFAGCTVHFVDENLDAGPIVLQAVVPVLD
                     EDTEVSLSETILKEEHRIYSEAVKIVLEGKYKIAGRRVLR"
     gene            3203..4627
                     /locus_tag="VGR03_05450"
     CDS             3203..4627
                     /locus_tag="VGR03_05450"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEV1993756.1"
                     /translation="MVNSMSRHREHSRTLFAFVFFAFALGAIPAIAQQAIPRDPTEPT
                     QGKPQPKILARPAGGVARASVDEQSMRTLIGKLVACGTRLTLSSWTDAKRGIGCGRDA
                     IVARFNEIAKDSGGKLQVVVDKFESTSERTSGKPMPLENVYAILPGSDPKLAKTIFIV
                     SGHFDSRPSNVMDPQADAPGADDDASGVAVSVECARLLNKAGASGRGTYRATILFAAV
                     SGEEQGLLGSTHLLEWAKQQGYTVGGMLDDDIVGADSAAGAPHRVRLFSGNGEIDDAD
                     SPSRELARAVEEIDGRAAIRMIFRVDRYGRGGDHYPFYKAGLPAVRFTEPLEDYNHQH
                     QTPRVENGIEYGDFEKHLNFTFMREVARGNAEALRQLALAPVPPMNAKLTGAVTPDAK
                     VSWSAEDDAERAGFEILWRETSDPRWQVYDFAASPGETVLKGVSTDNHFFAVRAVGKN
                     GARSIAVPTEMERRPPPLPAPSKQ"
     gene            complement(4808..5404)
                     /locus_tag="VGR03_05455"
     CDS             complement(4808..5404)
                     /locus_tag="VGR03_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993757.1"
                     /translation="MRYLRYLALLGIFMLTASYSQAQVSVGIGVGPGYGYVGAAPVCS
                     YGYYSYYPYECAPYGFYGPDYFVGGLFIGAGPWYHGYYGRGGYGYYGRGRGYNRGYDN
                     DRYYGRGYGYNGRGNGYSGRSDGNYGRGNGYSGHNNGSSGRGNGYSGRGNDNSGRGGN
                     GHGGAGGRGSAGGDNHGGHGGEGSYGNRGGESHGGGHR"
     gene            complement(5692..6666)
                     /locus_tag="VGR03_05460"
     CDS             complement(5692..6666)
                     /locus_tag="VGR03_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV1993758.1"
                     /translation="MLTDAVSKAVLVSAPEGLFVFDRQGRIVFVNSRIEELFGYAPGE
                     LLGQSLEVLLPDLFQEMPEPQSAASAPAPGLPAERIGLEQKGYRKDRAEILLQVSIGS
                     VETHDGPLACCIVCKIATPEQQNETLSELNCHFRQMVENSHDILTIRDADGRVRYTSP
                     SIHNVLGYKQEEMIGSTGFELIHPEDRSTVENALNEFWKNTGARDSIQYRAKHANGTW
                     VSLEVVAYNLLDHPVIRGVVLNGRDISQRKQEEAGKDQMIAELQQTITSIKSLTGVLP
                     ICASCKKIQEGDKWRQIEAYIRDRSQVEFSHTMCPECTGLWYPDLYQR"
     gene            complement(6894..>7088)
                     /locus_tag="VGR03_05465"
     CDS             complement(6894..>7088)
                     /locus_tag="VGR03_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993759.1"
                     /translation="AEARKGPTYLYIEVDDFDAVLAAMKDVKIVMPVRTAFYGMKEFS
                     VQDPGGHFITFAQPVAAPQH"
BASE COUNT         1480 a         1841 c         2245 g         1522 t
ORIGIN      
        1 ctattacccg aagaattcgc tgaaggcgcg gctgtgcgtc gagggcaatc ggcttaccta
       61 cgaattttgc gcgaagcata aagttccatt tcggcactgc gggaagctcg tggtggctgt
      121 ggatgcgcgc gaggaagccg atctgatcgc gctgaagaag cgcggggaag agaatggcgt
      181 tgcagggctg cggctgatcg gcgctgacga aatccgggcg cgcgagccgc acatcaaagg
      241 atttgcggct ctcgacgtgc cttcgaccgg gatcgtttcg gcagaggaac tggttcacgc
      301 ttatgcgcgg gtggcaacga atcagggagc gaatattgtc acgcgtgcgc aggtggtttc
      361 gctggagccc tccggcaaga gtgttcgcgt ggggctgcgc atcggcgacg aagaggatgc
      421 acagaacgaa acgatcgaag cgcggtgcgt gatcaatgcg gcggggcttt atgcggatga
      481 agtggcggcg atgctgggga atcactcgtg gaagatttat ccagtgcgag gagaatattg
      541 cgaggttcgc ggaccgcggt cggcgctgat taacaatctt gtttacccac tgccgcactc
      601 ggacgggctg agcctgggag tgcacttcac aaaaacgctg tggggaactt ttctgctggg
      661 gccgacggcg acgtacgtcg agggaaaaga caattacgaa aaaaaccgct tgccgattgc
      721 agattttgcg cacagcgcga aaacgctgct gcctgaaatc gaggagagcg acttacaact
      781 tgggtattcg gggctgcggc ccaaactcgt tccgccagat cacaaaggca tcgtggattt
      841 tgtgatcacg cgcgacaccg ctgtgccgca ggcgattcac ctcgtcggca tggagtctcc
      901 agggctcacg gccgcgcctt ccgtcgcgga acatgttgcg cagcttgtgg cagaaacgtt
      961 cgaatgagga ggaggtactg gcttgtgaaa tgtacgcccg tgcctagcaa ctgttttctt
     1021 ccctatggtg tgacgttcac tcgtaaggtt gtgaactttc cgacgcggag caaaaatatt
     1081 ctgggcgtgc ccagatcgat ctccgcggag acatcagtta gacgcgaatc atctatttgt
     1141 actgcaccct gttttatcaa tctttccatt tcacttcgag aagaatgcgt cagtttggcg
     1201 aagaatttga ctgctttgat gcgcgttgtt cctgggatca gatctggttt ccagccgtac
     1261 agcggcacat ttacaaccgg agcatcctct ggtgattcgc gcctgcggaa aactctttcg
     1321 aactcttcct tcgccatctg agcagagatc cgaccatgaa agccacttgt cacctccagt
     1381 gcgaggtcca tcttcacgtc catggggtgc gccgcgccgc tagacgtttg ctttttcaat
     1441 gcggcgattt cgtccggtgt gcgatccgtt accgcgtcgt agtaggacca catggctgag
     1501 tccgggatgg acattaactt gccgaacatt tcgctgggcg gttcggtgat gccgatgtag
     1561 ttgccaaggc tcttggacat tttgcggtgg ccgtcgaggc ccacgagaat aggcatggtc
     1621 agcgccactt ccgaggccaa gccgtactcg cgctggatgt cgcggtggac cagcagattg
     1681 aatttctgct cggtcgcacc gatctccaca tccgactgga gggccacggc gtcgtacgcg
     1741 gtcagcaatg gatacaacag ctcgtgaacg gaaatgggct ggttgttggc cagccgcgag
     1801 cgaaaatcct cacgttcgag catccgcgcc agccggtagt ggccgcacag ccggacgact
     1861 tcgtagctcg agagcttgtc gagccactcg ctgttgtagc gaacttcggt tttcttcgga
     1921 tcgaggagat tgaagacttg cgcgaggtag gtcttggcgt ttgcgttcac ctgttcgcgc
     1981 gagagcggcg gacgggtttc ggattgtccc gtcgggtcgc caatcatcgc ggagaaatcg
     2041 ccgatgagga aaatggcagt atgtccaagg tcctgaaagt gtttcagctt gcgaaggacc
     2101 accgtgtgtc ctagatgaat gtccggcgcc gtcgggtcca cgccgagata cacgcgcaag
     2161 ggctttccgg tcattcgcga tttctcgagc ttcgcggtta gctctgcctc gggaacgatt
     2221 tcggcgacgc cttttttcag gtaggcgagc tgttcttcga cgggtttgaa tggcgactgg
     2281 gtcattgtgt tttggtgatt ggcaatcgca ggcgctccag aaattcacag ggagcacaga
     2341 ctgaagtctg tgccacttgc actaccgaag cacgcggcgg ccggcgattt tgtattttcc
     2401 ttccaaaaca atttttacgg cttcggaata gatgcgatgc tcttccttga gaattgtttc
     2461 agagagcgac acttctgtat cttcgtcgag cacgggaacg acggcttgga gaacgatggg
     2521 gccggcgtca aggttttcgt cgacgaagtg aacggtgcag ccggcgaatt tcacgccata
     2581 ctcgagcgcc tggcgctgcg attcgagccc ggggaacgac ggaagcagcg aaggatggat
     2641 attgaggatg cggtttagat atttcgcgac aaaaaatggg gaaagcagcc gcatgtatcc
     2701 ggcgagacag attagatcga ctttttgttc gtccaagacc gcagcgacat ggcggtcgta
     2761 tacttcgcgc tccaggcctt tcgaaggaat cacgcgcgtc ttgattccgc gcgcttcggc
     2821 gcgttggata ccgggcgcgc cctctcgatt gctaaggacg atagcgatct ccgcgttcgg
     2881 aatcctaccc gccgcgacgc tttccgccag cgcctcgaag ttcgaaccac gcccgcttaa
     2941 cagtacgcct atgcgcttct tcataaacgc aaaacgttag cataaccgtt cgaatcggca
     3001 aaacggatgg acggaggttt gaagttgaaa attaaaagtt gaaaggggca acgtaactcg
     3061 tgactcgtgg aggatcacgg ccaggaagga ctgcgccacg aggaatgtcg cccactgccg
     3121 ttagactcgt gtggcctgga agcccaattg cgctagaatg ccgaattcgg gcaaagtcac
     3181 atcaatcggg gaagagataa tcatggtcaa cagcatgagc cggcaccgcg agcactccag
     3241 aactttattt gcatttgtgt ttttcgcttt tgccctcggc gcaattccag cgattgcgca
     3301 acaagcgatt ccccgggacc cgacggagcc tacgcagggg aagccacagc cgaagatttt
     3361 ggcgcgccct gcgggaggag tggcgcgtgc gtccgtggat gaacaatcga tgcggacgtt
     3421 gatcgggaag ctggtggctt gcgggacgcg gctgacgctt tcttcgtgga cggatgcgaa
     3481 gcgggggatt ggttgcgggc gcgatgccat cgtggcgcga ttcaatgaga tcgcgaaaga
     3541 ttccggaggg aagttgcagg tggtggtcga taaattcgaa tcgacttccg agcgaacttc
     3601 ggggaaaccg atgcctctgg aaaacgtata cgcgatcctt ccaggaagcg atcccaaact
     3661 ggcgaaaact atttttatcg tttcggggca ttttgattcg cggccatcga acgtgatgga
     3721 tccgcaggcg gacgctccgg gggcggacga cgatgcatcg ggagtggcgg tgagcgtgga
     3781 gtgcgcgagg ctgctgaata aggcgggggc aagcgggcgc gggacgtatc gcgcgacgat
     3841 actttttgcg gcggtttccg gcgaggagca ggggctgctt ggcagcacgc acctgctgga
     3901 gtgggcgaaa cagcagggct acaccgttgg cggaatgctg gacgacgata ttgttggcgc
     3961 cgattccgct gccggtgctc cgcatcgggt gcggcttttt tcagggaatg gtgaaattga
     4021 cgatgcggat tcgccttcgc gggaattggc gcgggcggtg gaagaaattg atggtcgtgc
     4081 ggcaattcgt atgattttcc gggtggaccg atacggccgc ggcggggatc actatccgtt
     4141 ttataaagcc gggctgccgg ccgtgcgatt caccgagccg ctcgaagatt acaatcacca
     4201 gcatcagacg ccgcgcgtgg aaaatggaat tgaatacggg gatttcgaga agcatctcaa
     4261 ctttacgttc atgcgggaag tggcgcgcgg taacgcagag gcgctgcggc agttggcgct
     4321 ggcccctgtg ccacccatga atgcaaagct aacaggagcc gtcacgccgg atgcgaaagt
     4381 ttcctggtcg gctgaggatg atgccgagcg ggcaggcttc gagatcctgt ggcgcgagac
     4441 cagcgatccg cggtggcagg tatatgattt tgcggcttcg ccgggcgaaa cagttttgaa
     4501 aggcgtgtcc acggacaatc acttttttgc ggtgcgcgcc gtgggtaaga atggcgcacg
     4561 ctcgattgcc gttcccacgg aaatggaacg gcggccaccg ccgctgcccg caccgagtaa
     4621 acagtgatca gttatcagtg atgggcaatc agaaatcagg aaccagtgag aagagggaat
     4681 aggggcgtgg tggcagcagg attattggaa tgtctcgaac tgcgatgaga agctgcctca
     4741 caaaggaaac ggctgacagc aactgccggc agccgttcct gttctctgtt ggataaagca
     4801 gggcagatta ccgatgtccg ccgccgtggg attcaccgcc ccggttgccg taggagcctt
     4861 cacccccatg accaccgtgg ttgtcaccgc ctgctgagcc gcgtccacca gcgccgccat
     4921 ggccgtttcc accacgccca gagttgtcgt tgccgcggcc ggagtagccg ttgccacgac
     4981 cggaggaacc gttgttgtgg ccagagtatc cgttgccgcg cccatagttg ccgtcgctgc
     5041 gcccggagta gccgttgcct cgaccgttgt agccgtagcc acgcccatag tagcggtcgt
     5101 tgtcatagcc gcgattgtag ccacgaccgc gtccgtagta gccgtatcca ccacgaccgt
     5161 agtagccgtg gtaccacggg ccggcgccga taaagagacc gccgacaaaa tagtcaggac
     5221 cgtagaagcc atacggcgcg cattcgtaag ggtagtagct atagtaaccg tacgagcaaa
     5281 cgggagctgc gccgacgtag ccgtagcccg ggccgactcc aatgccaacg gatacttgag
     5341 cctgggagta acttgcggta agcatgaaaa tgccgagtaa agcgagatac cttagatagc
     5401 gcattttaga cctttcctca gatccggaag acctgtccta aacctaacgg gatctgaagg
     5461 cctactggcg cctcaggtta gtctgaagcg ccccctgtag ttgttaaacc ccaagacggg
     5521 gaaagagttg caggcctttc cagtgcgata aagggcctta ccagcggtaa ttgaatggcc
     5581 gaccagtgac aggatgcgtt ctagtgcagg ggcgcggcga cgggttgggc gaaggtgatg
     5641 aagtggccgc cggagttcgg aacgtcgcga gtaagcagcg atccgtaacg gctatctttg
     5701 gtagaggtcc ggataccaga gcccggtgca ttcggggcac atggtgtggg agaactcgac
     5761 ctgggagcga tcccggatgt aggcctcgat ttgccgccat ttgtcgcctt cttgaatttt
     5821 cttgcaagaa gcgcagatgg gcaaaacacc agtgagagat ttgatgctgg tgatggtctg
     5881 ctgcaactcg gcgatcatct gatctttgcc ggcttcctct tgcttgcgct ggctgatgtc
     5941 gcgaccgttg aggaccacgc cgcgaatgac cgggtgatcc agcagattgt aggcgacgac
     6001 ttcgagagag acccaggttc cgttcgcgtg tttagcgcgg tactggatgg aatctctcgc
     6061 gccggtattc ttccagaatt cgttcaacgc gttttccact gtggagcggt cttcgggatg
     6121 aatcagttcg aaacccgttg agccgatcat ttcttcttgc ttgtacccca atacgttgtg
     6181 gatggacggg ctggtgtagc ggacgcggcc gtcggcgtcg cggatggtga gaatgtcgtg
     6241 gctattctcg accatctggc ggaaatggca gttcaactcg cttagcgttt cgttctgctg
     6301 ctcgggagtg gcaatcttgc acacgatgca gcacgccaaa ggcccgtcat gggtttcaac
     6361 ggagccgatg ctgacctgga gcagaatttc cgcgcggtcc ttacgatagc ccttctgctc
     6421 gagcccgatt cgctccgctg gcagtccggg agcgggggcc gaggccgctg actgcggctc
     6481 gggcatttcc tggaacagat cgggcagcag aacttcgagg ctttgcccga gcaattcgcc
     6541 gggagcgtag ccgaaaagct cttcgattcg tgagttgacg aagacgatgc gtccttggcg
     6601 atcaaagacg aagagtcctt ccggggcgga aacgagcacg gctttggata cggcatcagt
     6661 gagcataagt ctccctggtg ttatgactct ttcttaaaaa cagaactgca ggagttgacg
     6721 aacgggcgag tccgtcgact gcggatcgaa tcggatagca cgcgcaacga aatgcaagag
     6781 tagatttgcg cgcgtgggct gtcaaagccg cgcgtactgg tacagtaaat ggtacgagga
     6841 caggtacgag gcgatagtac ttggcctcgt ttggccgagc ctgacgccgc gcgctagtgc
     6901 tgcggggcgg cgacgggttg ggcgaaggtg atgaaatgac cgccggggtc ctggacgctg
     6961 aattccttca tgccgtagaa ggcggtgcgg acgggcatga cgatttttac gtctttcatg
     7021 gccgcgagca cggcatcgaa atcgtccacc tcgatataca gatacgtggg gccctttctg
     7081 gcttccgc
//
LOCUS       DASYVO010000164        12411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_132332, whole genome shotgun sequence.
ACCESSION   DASYVO010000164 DASYVO010000000
VERSION     DASYVO010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 12411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12411
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132332"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VGR03_05470"
     CDS             complement(<1..229)
                     /locus_tag="VGR03_05470"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEV1993760.1"
                     /translation="MNLAQRLVSFPDPRRRGRAAIALRAAGFTLLEMMIVIAIIMILV
                     GIAAVRYDRSVKRAKEAALKSDLKTMRQAIEQ"
     gene            complement(237..722)
                     /locus_tag="VGR03_05475"
     CDS             complement(237..722)
                     /locus_tag="VGR03_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV1993761.1"
                     /translation="MWPRKRRFRRGDPQAGLTLLELILACSILLILSSAAVPIARHNI
                     VRQKEAELHRDLREMREAIDRYKDTCDKNLIRSEQGSECYPPDLDTLVKGVQLGAGND
                     RKIRFLRRIPVDPITGRAEWGLRAIQDDPDSTAWGGKNVFDVYTKSYATSLDGTRYAG
                     W"
     gene            complement(741..3002)
                     /locus_tag="VGR03_05480"
     CDS             complement(741..3002)
                     /locus_tag="VGR03_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HEV1993762.1"
                     /translation="MRRLGSLLLVVTCGLLAAGCPRSKGTEDYKQARRAESLKDYDTA
                     LEHYQKALNADPFNADYKIKTGQLRFEASQYHVRQGQKLREKGELQLALAEFQKASAI
                     DPSSPIAEQEIRITLERIAVRNKEAEPAAAPPGQSTESPLSEGPPHLMPLSRAPINLK
                     MANDAKIIFETIGKMAGLTMIFDPEYPARRVVTELNNVSLEQALDIVSLQSKSFWKPI
                     TQNIIFVTQDQPTKRRDYEEQIIRTFYLSNTVQPQELTEIVTSIRQILDLKRIFQLNA
                     QNAIIIRDTPDKMTLAEKLINDIDRAKPEVIIQVEVLQARLDRLRDLGILPTQSATLS
                     FAPTGAIPVAGAGAAAPTAAGLALNKLHKLSTADYSVTLPGATATAIMTDSTTRLIQN
                     PEIRSVDGQPAKLKIGDRIPVATGSFQAGVGVGATGATGIVNPLVNTQFTYIDTGVNI
                     EVTPRVHPNRDVSLKITVDISSVTGQSAIGGIQQPIISQRKIDHDIRLKEGEVSILGG
                     MIDRTETKTLKGWPGLSKIPFFRYFVSDENKEIQESEILIVLIPRIVRLPEWTRASLR
                     PLSSGSEAQVQVRRESSLEAPKLQPQPPATLPSPAAQTQQSAGAPAAASPARLRFEPQ
                     SQTLKVGQTTTLAIVVENVQDLFSIPMLLQYNPAVLSVEEVRHGGFLSGGTQEIALVQ
                     TVNKERGQAIISATRQPNSQGVSGNGTLLGLVVRALAPGSSSVSIVQVSAKDSQLKAI
                     PLVSGEAAVQVVP"
     gene            3128..3616
                     /gene="smpB"
                     /locus_tag="VGR03_05485"
     CDS             3128..3616
                     /gene="smpB"
                     /locus_tag="VGR03_05485"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV1993763.1"
                     /translation="MARAAKKVEKNGELMVAQNRAASYHYHLLERFEAGMVLHGTEVK
                     ALREGKANIRDAYVAVKANGAWLENAHIAQYLPGGPWNHEPLGSRKLLLHRNELRKLA
                     GRAQIKGLTVIPLRIYFRKGIAKCEIALAKGKKDYDRRQDARDKEARREAEEALYRSR
                     RR"
     gene            3624..5114
                     /locus_tag="VGR03_05490"
     CDS             3624..5114
                     /locus_tag="VGR03_05490"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEV1993764.1"
                     /translation="MKITLEPRPYAEVETEALISYVFEEADPVQGRIGELDRKTGGLL
                     RKLAQGGEITGKLLEMTLVHAPAGLKAQRLLLVGAGKSAQYGTPQLRKLAGAALRFLK
                     ARAVKRCAFVVREADATPESAQAATEGLIFADFESDKYKSEKKDGKEIEAVMLCGWEG
                     KERAAGEKGLERGRIIAEAQNFTRDLVNEPSNRLTPALLAARAAEMAKVAGLACEILD
                     EKRIAELKMGALLGVAQGSDEPPRVMVVTYTPANPKAGAPVIGLVGKAVTFDTGGISI
                     KPAEGMEKMKYDMAGGATMLGVMRALAALKPSVKVVAVVSSTENMPGGRAQKPGDVQV
                     SMSGKTIEVLNTDAEGRLILADAITYAKQLGCTHLIDAATLTGAIVVALANVNVGVFG
                     SEQEFTDKVLASAKVCGEKMWPMPLDEDYLEFIKGTMADIQNIGSGKGGGAITAAMFL
                     KEFTGETPWVHLDIAGTAWFDEAKPWAAKGASAVAVRTLVDLVMKQ"
     gene            complement(5140..7107)
                     /gene="argS"
                     /locus_tag="VGR03_05495"
     CDS             complement(5140..7107)
                     /gene="argS"
                     /locus_tag="VGR03_05495"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV1993765.1"
                     /translation="MYQTLTTRLREALSRHIQQNYALTLDVVLEKPPKLEMGEAATPV
                     CFELAKRLKRGPRQIAQEIVANFALPKGIARLEVAGGGYLNAFFDRAAFWHSAVREAS
                     GEAGVSTALPGKIIVEHTNINPNKAAHIGHLRNAVLGDTFVRLLRHAGHTVEVQNYID
                     NTGVQVADVVIGFLHMEKRTPADVRTLSKKPRFDYYCWDLYARVTHFFEEDVSRQALR
                     GETLKRIEEGRGEEAEIAEVVADTVVACHLRTMSRLGVAYDLLPRESEILHLHFWDAA
                     FAKLKETGAIQLSSSGKNAGCWVMPWKESSGGDAAAEDADAQAKIIVRSNGTVTYVGK
                     DIAYQLWKFGLLGKDFHYARYPHFPEGATTWITTSAASDPNAPAFGGAATVYNVIDTR
                     QAYLQQVVTAGLRALGHTAEADRSIHFAYEVVALSPRCARELGYELSPEDAKKPYVEV
                     SGRKGLGVKADDLLDRLEAATLAEVLERHPQMGGVEQQQTAHAVAVSALRFFLLKFTR
                     NAIIAFDFKDALSFEGETGPYCQYAVVRIRGIRRKGAEAGGNSASLSDEFLSQVFSGA
                     GGDGLWEVAVLSGSLDQAVEAAIGAQEPAFLAKYAFQLAQSFNNFYHKHHILSEADAQ
                     KRAFLLRLTELVEQQLVRALDLMGIDCPEKM"
     gene            complement(7536..8087)
                     /locus_tag="VGR03_05500"
     CDS             complement(7536..8087)
                     /locus_tag="VGR03_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993766.1"
                     /translation="MSAPRSKDRTRLCVFTFADGRKCRTPRQPAVAGQPGHPHLCFYH
                     ARKEAQARAADQVGREISSSLTSSYLSACDLNSALARLFAAVAQGHVKPRTAATLAYL
                     GQTLVQTIPLAQDEYINAFGTDGWREAVRSSFPGQPYEDEDQVLDGADHPELPAGAKL
                     SSPSDSALLTSLESTLAKLYQNK"
     gene            complement(8542..10563)
                     /locus_tag="VGR03_05505"
     CDS             complement(8542..10563)
                     /locus_tag="VGR03_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993767.1"
                     /translation="MEEQERNHGARKLIAAISFYDLGQRKPSREELVEMAKRFNYEAG
                     AAFLAWLNVCLSLAMASSDDKVVVEAHKKLTSNVLSPSRLQEIASAFGDKLYQSAILL
                     NRAQLLSGIKLLALYAQREGGNTLETEVERHQVGELALAINSFYGPDIGEPNWPAGDV
                     SVQLMASAELNNPEPILNGLVRTRCLLGPVLEEYLERLKGQVPPPPFERIFTLLNGLN
                     FRDFLDITLYLYLELSRMLDELMSTGQMAYIDIRLPKRYVSGKNMANWAQPMAASLDD
                     LPSLVRGSEADIAFFFDMTIFRQFPLWRSGGYKYFLIDPMFLAERLSSYGFYWTVVNG
                     LADDRLRYQFQCLWGELIQEYERQLLAESFIAQPGSFLRRPIYSDDGSEVFDCAVLLG
                     DGLIPIEIKGSVLPTADKYAAKAGPFYQGVSAKFGSGPGAAVEQLLRNIEHILSAEHP
                     RESSQIPSGRIRRILPIVIVHERIFRFGGLARTLGTEFTTGLMRLKIRPPLERLEIYP
                     FQLLTVEELEHLQPYIQERDFDLFDCVRSKAKEDPDYQLGLWEFITTRFLRAKGIKPR
                     ANEKMIGRFKWLTKAQSWRVYRGDYYDHSLAERRKATDYAIISARPIGGDKLLWDEVI
                     AYKEYPTAGEAYEAIREIADSEFPKQPISADWFECLVVDEFGVPIEKPT"
     gene            10893..12086
                     /gene="larC"
                     /locus_tag="VGR03_05510"
     CDS             10893..12086
                     /gene="larC"
                     /locus_tag="VGR03_05510"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEV1993768.1"
                     /translation="MKLVYFDCFSGISGDMVLGALVDAGCPLEELRAGLSGLALSGWE
                     ISAEKVWKNGMAATYVRVGTEEQAKHRSLSSILEILEKSSLAAPVKERAAAIFRRLGE
                     AEAKVHDVPIEKVHFHEVGALDAIVDIVGAAIGFQTLGIERFACSALNVGSGTVKTAH
                     GVLPVPAPATAELLRGTPTYSSGVERELVTPTGAAIVATLCEEFGPLPRMSVAAIGYG
                     AGTADLPGQPNVVRILVGEAAESGASIGTAEEVAVIEANLDDMNPQIYGYFLEKALAA
                     GALDVYTTPVQMKKNRPGALLTVLCKPENTDVILKLIFAETTTFGVRTYRAERRVLPR
                     EWVTVETAFGDVRMKLSRANGRILRAAPEYEDCRKLATERDVPLQRVMAEAMRNYEEQ
                     VRKGG"
     gene            12136..>12411
                     /locus_tag="VGR03_05515"
     CDS             12136..>12411
                     /locus_tag="VGR03_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEV1993769.1"
                     /translation="MAGTKPKFYLTTPIYYTNGLPHIGHTYSTIVCDTIRRYKRMRGY
                     DVVLTTGTDEHGVNVERSARKAGKSEAEFVNEMAGEWRRLWDELGIQG"
BASE COUNT         2556 a         3230 c         4214 g         2411 t
ORIGIN      
        1 attgctcgat ggcctggcgc attgtcttca ggtcggactt gagagcggct tccttggcgc
       61 gcttcacgga gcggtcgtac cggactgccg caatgccaac gaggatcatg atgatggcga
      121 tgacgatcat catctcgagc agggtgaatc ccgcagcccg cagagcgatt gccgcgcggc
      181 cccggcggcg aggatccgga aaagaaacga gacgctgtgc gagattcatg ggcatctcac
      241 caaccggcgt agcgggtccc gtcgagcgag gtggcgtagg atttagtgta gacatcgaag
      301 acgttttttc caccccaggc ggttgagtcc gggtcgtcct ggatggcgcg gaggccccat
      361 tcggcgcgtc cggtgatggg atccacgggg atgcggcgga ggaagcggat cttgcggtca
      421 tttccggcgc cgagctgcac ccccttgacg agtgtatcca gatccggagg atagcactcg
      481 ctgccctgtt cggagcggat gaggttcttg tcgcaggtgt ccttgtaacg gtcaatcgcc
      541 tcgcgcatct cgcgcaggtc gcgatgcaac tcggcttctt tttgccgaac gatgttgtgg
      601 cgggcaatgg gcacggccgc ggaagaaagg atcagcagaa tcgaacaggc gaggatcagc
      661 tcgagcaggg taagccctgc ttgaggatcg ccgcgccgga atctgcgttt cctgggccac
      721 atagggctga agaagaatag ctagggcacg acctggactg ccgcctcgcc gctgacaagc
      781 ggaatggcct tgagctggga gtccttcgcg ctgacctgga cgatgctcac gctcgatgag
      841 cccggagcga gagcgcgaac gaccaagccg agcaaggtgc cattcccgct gacaccctgg
      901 ctattcggct ggcgcgtggc ggagataatc gcctgaccgc gctccttatt cacagtctga
      961 acaagggcga tctcctgcgt gccgccggaa aggaatccgc cgtggcggac ctcctcgacg
     1021 ctaaggacgg cggggttgta ctgcaggagc atgggaatcg agaagagatc ctgaacattt
     1081 tcgacgacaa tggccagtgt cgtggtctgg cccactttga gggtttgcga ctggggctcg
     1141 aagcgcaggc gcgctggtga agccgctgcc ggcgcccctg cagactgctg tgtctgcgcc
     1201 gcgggagatg gaagcgttgc gggaggttgc ggctggagct tcggagcctc gagactgctc
     1261 tcgcggcgaa cctgaacctg ggcttcggag ccggagctga gcggcctgag actggcgcgg
     1321 gtccactccg gtagccggac aatacgcggg atgagaacaa tcaagatttc gctttcctga
     1381 atctctttgt tctcgtcgga tacgaagtag cggaagaagg gaatcttgga gagacccggc
     1441 cagcccttga gggtcttcgt ctccgtgcgg tcgatcatgc cgcccaaaat gctgacttca
     1501 ccctctttca ggcgaatgtc gtgatcaatc ttacgctggc tgatgatggg ctgctggatg
     1561 ccgccgatcg ccgactggcc cgtaacggag gaaatatcca ccgtaatttt caggctgaca
     1621 tcacgattgg ggtggacacg cggcgtgacc tctatgttta cacccgtgtc gatgtaagta
     1681 aactgggtat tcacaagcgg gttgacgatg cccgttgcgc cggtcgctcc gactccaacg
     1741 ccagcctgaa agctgcccgt cgctactgga atacggtcgc cgatcttgag tttggccggc
     1801 tgaccgtcaa cggagcggat ttccggattc tggatgagcc tggttgtaga gtccgtcatg
     1861 atagcggtcg ccgtcgcacc ggggagggtg acgctgtaat ccgccgtaga cagcttatgc
     1921 agtttattca gggcgagtcc cgcggcggtc ggagcggcgg ctcccgcgcc ggccacaggg
     1981 atggcgccgg tgggcgcgaa ggaaagtgtg gcggactggg tcgggaggat gcccaaatcg
     2041 cggaggcggt cgaggcgagc ctgcaagact tccacctgga tgatgacttc gggtttggct
     2101 ctgtctatat cgttgatcag cttttcggcg agggtcatct tatccggagt gtcgcggatg
     2161 atgatggcgt tctgcgcgtt caactgaaag atgcgcttga gatcgaggat ctggcggatg
     2221 ctggtgacga tctcggtgag ctcctgcggc tgaacggtat tggagaggta gaaggtgcgg
     2281 ataatctgtt cttcgtagtc gcggcgcttg gtaggctgat cctgcgtgac gaagataatg
     2341 ttctgggtga tgggtttcca gaaagatttg ctttgcaggg agacgatgtc cagcgcctgt
     2401 tcgaggctga cgttgttcag ctccgtgacc acacggcggg caggatattc cggatcaaag
     2461 atcatcgtga gcccggccat tttcccgatc gtttcaaaga taatcttcgc atcgttggcc
     2521 atcttcaagt tgatcggtgc ccgggaaagg ggcatgagat gcggcggtcc ctccgagaga
     2581 ggagattctg tgctctggcc cggaggagcc gcagccggtt cggcctcttt gtttcttacg
     2641 gcgatccgct caagggtgat ccggatttcc tgctcggcga tggggctgga ggggtctatg
     2701 gcggaggcct tctggaattc tgccagggcc aactggagtt cacccttctc gcggagtttc
     2761 tgtccctggc ggacatggta ctggctcgct tcaaaacgaa gctgaccggt cttgatcttg
     2821 tagtcggcgt tgaaagggtc ggcgttaagc gccttctgat agtgctcgag cgcggtgtcg
     2881 tagtctttca gcgactccgc ccggcgggct tgtttatagt cctctgtgcc tttgcttctg
     2941 gggcaccccg ccgccagcag gccgcaggta acaacaagaa ggagactccc caagcgacgc
     3001 atggatactc caagggctgc agatgccccg caaaccaggc agtgtagcat tcgattagag
     3061 ggcgggcaag caagtatggg ttggctgcct tgcgtcgagc gagaggcgac cgttaagatg
     3121 aaacatcgtg gcccgagcgg caaagaaggt agagaagaac ggggagctga tggtcgcgca
     3181 gaaccgcgcg gcgtcgtatc actatcactt gttggagcga tttgaggcgg ggatggtgct
     3241 ccacgggacg gaagtgaagg ccttgcgcga agggaaggcg aatatccgtg acgcctatgt
     3301 ggccgtgaag gcgaacgggg cgtggctcga gaacgcccac atcgcgcagt acctgccggg
     3361 aggcccgtgg aaccacgagc cgctcggctc gcggaagctg ctcctgcacc ggaatgaatt
     3421 gcgcaaactg gcaggccgcg cgcagatcaa aggtttgacc gtgatccctt tgcgaatcta
     3481 ttttcgcaaa ggcatcgcga agtgcgagat tgctctcgcg aaaggcaaga aggactacga
     3541 ccgccggcag gacgcccgag ataaagaggc gcgccgggaa gccgaagaag ctttgtatcg
     3601 ttcccggaga cgataggagg agcatgaaga ttacattgga accccgcccg tatgcagagg
     3661 tcgagacgga ggcgttaatc agctatgtgt tcgaagaggc agaccctgtg cagggacgaa
     3721 tcggggagtt ggaccgcaag acaggcgggc tgctccgcaa actggcgcag ggcggagaga
     3781 tcacagggaa actgctggag atgacgttgg tgcatgcacc ggcggggctg aaagcacagc
     3841 ggcttctcct ggtgggcgcc ggcaagagcg cgcagtacgg cacaccgcag cttagaaaac
     3901 tcgccggagc ggcgctgcga ttcctgaaag cgcgtgccgt gaaacggtgc gcctttgtcg
     3961 tccgagaagc cgacgcgacg ccggaatcgg cgcaggcggc aaccgagggg ctgatcttcg
     4021 ccgatttcga atcggacaag tacaagagcg agaagaaaga cggaaaagag atcgaggcgg
     4081 tgatgctttg cggctgggaa gggaaggagc gcgctgccgg agaaaagggc ctcgagcgag
     4141 ggcggattat cgcggaggcg cagaacttca cgcgggacct ggtgaacgag ccgtcgaacc
     4201 ggctgacgcc ggcgctactg gcggcccgcg ccgcggagat ggccaaggtg gccgggctgg
     4261 cctgcgaaat cctggatgag aagcgcatcg ccgagttgaa gatgggagcg ctgctcggcg
     4321 tggcgcaagg gagcgacgag ccgccgcggg tgatggtggt cacctacacg ccggcgaatc
     4381 caaaggccgg ggcgccggtg atcgggctgg tggggaaagc cgtgacgttc gataccggag
     4441 gaatctcgat caagcccgct gagggtatgg agaagatgaa atacgacatg gcgggcggcg
     4501 cgacgatgtt gggggtgatg cgcgcgctgg cggcgctgaa acccagcgtg aaagtcgtcg
     4561 cggtggtgag ttcgacggag aatatgccgg gcgggcgggc gcagaaaccc ggggatgtgc
     4621 aggtgtccat gtccgggaag acgatcgaag tgctgaacac ggacgcggaa ggccggctga
     4681 tcctcgccga cgccatcacc tatgcgaagc agctcggatg cacgcacctg atcgacgcgg
     4741 cgacgttgac gggcgcgatc gtggtggcgc ttgcgaacgt gaatgtgggc gtgttcggct
     4801 ccgagcaaga gttcacggac aaggtgctgg cgagcgcgaa ggtctgcggc gaaaagatgt
     4861 ggccgatgcc gctggacgaa gactatcttg aattcatcaa aggcacgatg gccgacatcc
     4921 aaaacatcgg cagcggaaaa ggcggcggag cgattacggc ggcgatgttc ctgaaggagt
     4981 tcacgggcga gacgccgtgg gtgcatctgg acatcgcggg cacggcgtgg ttcgatgagg
     5041 cgaaaccgtg ggcggcaaaa ggcgcaagcg ccgtggccgt tcgcacgttg gtggatctgg
     5101 tgatgaagca gtagccggcg ggcgtgaggc ggcgggaacc tacatctttt cgggacagtc
     5161 gattcccatc agatcgagcg cgcggacgag ctgttgttcg accagttcgg tgaggcgcag
     5221 gaggaaggcg cgcttctgcg cgtccgcttc ggagagaatg tggtgcttgt ggtagaagtt
     5281 gttgaaggac tgtgcgagtt gaaaggcgta ttttgccagg aacgccggct cctgcgcgcc
     5341 gatggctgcc tccacggcct ggtccagtga accgctgaga accgcgacct cccagaggcc
     5401 gtcccctccg gctccggaaa agacctgcga caagaactcg tcggagagcg acgctgaatt
     5461 cccgccggcc tcggctcctt tgcggcggat gccgcgaatc cgcacaaccg catactgaca
     5521 ataggggccg gtctcgccct cgaagctgag agcatctttg aaatcgaagg cgatgatggc
     5581 gttgcgcgtg aatttcagaa gaaagaaacg cagagcgctg accgcgacgg catgggccgt
     5641 ctgctgctgt tcgacgccgc ccatttgcgg atggcgctcg aggacttcgg cgagcgtggc
     5701 ggcttcgagc cggtcgagga ggtcgtcggc cttgacgccg agccccttgc ggccgctgac
     5761 ttcgacgtag ggttttttcg cgtcttcggg agaaagctcg tatcctagtt cgcgtgcgca
     5821 gcgtggcgag agggccacga cttcgtaggc gaagtggatc gagcgatcgg cttccgccgt
     5881 gtggccgagc gcgcgcaggc cggcggtgac aacctgctgg aggtaggcct ggcgcgtgtc
     5941 aatcacgttg tagacggtgg cggcgccgcc gaaggcgggc gcgttcggat cgcttgcggc
     6001 gctcgttgtg atccatgtcg tcgcgccttc cgggaaatgc ggatagcgcg cgtagtgaaa
     6061 atcttttccg aggaggccga atttccagag ctggtaggcg atgtccttgc cgacgtaggt
     6121 gacggtgccg ttcgaccgga caatgatttt tgcctgcgcg tccgcatcct ccgcggcggc
     6181 atcgccgccg gagctttcct tccagggcat gacccagcag ccggcgttct tgccggaaga
     6241 ggaaagctgg atcgctccgg tttcttttag tttggcgaac gcggcatccc agaaatggag
     6301 atgcaggatt tcgctttcgc gcggaagcag gtcataagcg acgccgaggc gcgacatggt
     6361 gcgcagatga caggcgacga ccgtgtcggc gacgacctcg gcaatttcag cttcctcgcc
     6421 gcggccttct tcaattctct tgagcgtttc gccacggagg gcctggcgtg aaacgtcttc
     6481 ctcgaaaaag tgcgtgaccc tggcgtagag atcccaacag tagtaatcaa agcgaggctt
     6541 cttcgagaga gtacgcacgt cggccggcgt gcgcttctcc atgtggagga agccgatgac
     6601 gacatcagcg acctgcacgc cggtattgtc aatgtagttt tggacttcga ccgtgtggcc
     6661 ggcgtggcgc aggaggcgga cgaaagtgtc gccgagaacg gcgttgcgca aatggccgat
     6721 gtgagcggcc ttgttcggat tgatgttggt gtgctcgacg atgatcttgc cggggagggc
     6781 agtagagact cccgcctcgc cggaagcttc acgaacggcg ctatgccaga aagcggcgcg
     6841 gtcgaagaag gcgttgaggt agccgccacc ggcaacctca agccgggcga tgcccttggg
     6901 cagagcgaaa ttcgcgacaa tctcctgggc aatctggcgc ggaccgcgtt tcagacgctt
     6961 ggcaagctcg aagcagacgg gagtggccgc ctcacccatc tccagcttgg gcggcttttc
     7021 caggacgacg tcgagggtca aggcgtagtt ctgctggatg tgacgcgaga gagcttcgcg
     7081 gaggcgagtt gtgagcgtct ggtacacgat tcctcgacgg atgagtgcgt cagtataggg
     7141 gaggtgtggg agggggtcaa agcgcgcttc ggagtggcgg ccccaaatga tgcccggaga
     7201 gatcccttcc gccaggaacg gaaagaggat tcaccacaga gagcacagag gccacagagt
     7261 gcggatgcga ggcaggaacg ggatctccga tagtcatgcg ctgctaggcc gccacggagg
     7321 gctcggtcag ggttcgcatc ctggtcggat gcgaagagag atccctcgtc gcgatgctcc
     7381 tcgggactcg catcctagtc ggatggcagg agaggtccct cggctccccc gccggaggcg
     7441 ggctcgggac tgccatcctt gtcggatggc aggttaacaa tatgaccccc tccccccggt
     7501 ttttttgtaa gtgttgattg caaagggggt taaagtcatt tgttttgata cagttttgca
     7561 agtgttgatt ctaaggaagt tagcaaggcg ctgtcgctcg gggagctcaa cttggcgccg
     7621 gccggtagtt ccgggtgatc ggctccgtcc aggacttgat cctcgtcctc atagggttgg
     7681 ccggggaaag aggaacgtac ggcttcgcgc cagccgtcgg tgccgaaagc gttgatgtac
     7741 tcatcctggg cgagcgggat ggtttgcacg agggtctggc cgagataggc gagggtggcg
     7801 gcggttctgg gcttgacgtg gccctgggcg acggcggcga agaggcgcgc cagagcggag
     7861 ttgagatcgc aggcggagag atagctgctg gtaagggagg aagagatctc gcgaccgact
     7921 tgatcggcgg cgcgggcctg cgcctccttg cgggcatggt agaagcagag atgaggatgg
     7981 ccaggctggc ccgccaccgc gggttgtcgc ggagtgcggc acttgcggcc gtcggcgaag
     8041 gtgaagacac acaaacgggt gcggtccttg gagcgaggag cggacatggg agtctccaga
     8101 aaagaaggat ttggatggga agggaggtcc tcgcaggaag cgagaggccc aattacccaa
     8161 cagaattatg caacaatggt aatcgaattg tcaagggaat tctgctgtgc gaaggaaggg
     8221 aaacgggagg ttcaccacag aggacgaagg gggcacagag agtgggcacg gctgtcgcgg
     8281 tcgaggcgtg gaaaccggct caggttccga ttcggcacct ggatgaccgc gtgaacgcgg
     8341 aggtcagaag agccgaaatg tcctgcgatg gtttccgagt tgccgactat acgtccgttt
     8401 gcgagccttt accggctggc aagagcttag ggttcggaat ctgaaacaaa gcggacgccg
     8461 cggtacagac cttggtccgt gcggaagcac gtaggtgtcg ctactacggc cagcacgcct
     8521 ctcatcgcgg tgtttgttcc cttatgtagg tttctcaatt gggaccccga attcgtcaac
     8581 cacgagacat tcgaaccagt ctgcggaaat tggctgcttg ggaaattcgg agtcggctat
     8641 ctcccgaatt gcctcatacg cttcgccagc cgttggatac tctttgtatg cgataacttc
     8701 gtcccaaagc aatttgtcac caccgatggg cctagcacta atgattgcgt agtcggtcgc
     8761 ctttcgccgc tctgcgaggg agtggtcgta ataatcgccc cggtagactc gccacgactg
     8821 ggcctttgtc agccatttaa accgcccaat cattttctcg ttcgcacgcg gcttaatgcc
     8881 tttggcccgt agaaatcgag ttgtaataaa ttcccacaac cccagctggt agtccgggtc
     8941 ttctttcgcc ttggagcgca cacaatcgaa gaggtcaaaa tctcgttcct gaatgtacgg
     9001 ctggaggtgt tcgagttcct cgactgtcag gagttggaac ggatagattt caagacgttc
     9061 aagaggcggg cgaattttga gccgcatcag acccgtagtg aattcggtcc ccaacgttct
     9121 agcgagtccc ccgaatctaa aaatccgctc gtgcacgatc acaattggga gtatccgacg
     9181 gatgcggccc gagggtatct ggctcgattc gcgcggatgc tcagcggaaa gaatgtgctc
     9241 aatgtttcta agcagctgct caaccgccgc cccagggcca ctaccgaact tcgctgagac
     9301 cccttgatag aaaggccctg ccttcgctgc atacttgtct gcggttggga ggaccgaacc
     9361 tttaatttca atcggaatca gcccatcgcc gaggagtact gcgcagtcga aaacttcaga
     9421 gccatcgtca ctatagatgg gtcgacgtag aaagctgccc ggttgtgcga taaatgattc
     9481 ggccagtagc tggcgctcgt attcctggat cagctctccc cagaggcatt gaaattgata
     9541 acggaggcgg tcgtcggcga gaccgttgac caccgtccaa tagaagccat aggaggaaag
     9601 cctttcggcc aggaacatcg ggtcgatgag aaaatatttg tagccgccgg atcgccagag
     9661 tggaaactgg cgaaagatgg tcatatcaaa gaagaatgcg atatcagctt cggagccgcg
     9721 caccaaggat ggcaagtcat cgaggctcgc agccattggt tgagcccaat tcgccatatt
     9781 tttcccggag acatatcttt taggcagcct tatgtcgatg tatgccattt gacctgtaga
     9841 catcagctcg tccagcattc ggctcagctc aaggtatagg taaagcgtaa tgtccagaaa
     9901 atctcgaaag ttaagtccat ttagcagtgt gaagatcctc tcgaacggtg gcggaggaac
     9961 ttggccctta agacgctcta aatactcctc caagaccggc ccaagaagac atcgggtgcg
    10021 taccagacca tttaagattg gctcggggtt gttaagctcg gcgctcgcca ttagctggac
    10081 ggagacgtcg cctgctggcc aattcggctc cccgatgtca ggaccgtaga acgaattgat
    10141 tgcgagagcg agttctccta cttggtgtcg ctccacttcc gtttcgaggg tgttgccgcc
    10201 ttcgcgctgg gcgtaaagtg caagcaattt gatgccggac aagagttggg ctcggttcaa
    10261 gaggatcgcg ctttggtaca gcttgtcccc gaaggcgctt gcgatttcct gcagtcgtga
    10321 cggcgacagg acgttcgagg ttaacttttt atgtgcctcg acgacgacct tgtcgtcgct
    10381 ggaagccatt gcgagcgaca ggcaaacgtt taaccaggcc agaaacgcag caccagcctc
    10441 gtagttgaat cgtttggcca tttccaccag ctcttcacgc gagggcttgc gctggccgag
    10501 gtcgtagaac gagatcgcgg ctataagttt acgggcaccg tgattgcgct cttgctcttc
    10561 caattggacc tcggatcaca ggaggtttac ggcagatcca tgtacatttt gttagatagt
    10621 atagctgtcg gcggcattaa gcataccggg cgtgcgggta gaaacggaaa gtcgtttttt
    10681 tctactgagg aaacgcagcg tttctgacgg catagacggc gagtgcccgg atgacggcgc
    10741 actcggaaac aatccacttg actaagtaat gcgcggaatg gtcgtcgcgc cactagcgag
    10801 agcgagggcg atttgacacc cctcggatgc gccgatagta tccaatcgta gaggcgactt
    10861 cacgttgccg aggaatctct cggtgtaacc ccatgaaact ggtttacttc gattgctttt
    10921 ccgggatcag cggcgacatg gtcctcggcg cgctggtgga cgcaggttgt cccctcgaag
    10981 agctgcgcgc gggactgagc ggcctcgcgc tgagcggctg ggagatttcg gccgagaagg
    11041 tatggaagaa cggcatggcc gccacttatg tccgcgtggg gacggaggag caggcgaagc
    11101 accgctcgtt gagctcgatt ctcgagattc tggagaagtc gtcattggcc gcgccggtga
    11161 aagaaagagc ggcggcgata ttccggcggc tgggcgaggc ggaagcgaaa gtacacgacg
    11221 tgccgattga aaaagtgcac ttccacgagg tgggcgcgct ggatgcgatt gtagatatcg
    11281 tgggcgcggc gattgggttc cagacgctgg gaatcgagcg gttcgcgtgt tcggcgctca
    11341 atgtgggaag cggaacggtg aagacggcgc acggggtgct gccggtgccc gcaccggcaa
    11401 cggcggaact gctgcgcggg acgccgacgt attcatcggg ggtggaaagg gaactggtga
    11461 cgccgacggg cgcggcgatc gtggccaccc tgtgcgagga gttcggaccg ctgccgagaa
    11521 tgagcgtcgc ggcgatcggc tacggcgcgg gaacggcgga tttaccgggc cagccgaacg
    11581 tggtgcgcat cctggtggga gaagcggcag aaagcggagc gtcgatcggg acggccgagg
    11641 aagtggcggt gatcgaggcc aacctggatg acatgaatcc gcaaatctac ggctactttc
    11701 tggagaaggc gctggccgcg ggagcgctgg atgtctacac aacgccggtg cagatgaaga
    11761 agaaccggcc cggagcgctg ctgacggtgc tgtgcaagcc ggagaatacg gacgtaattc
    11821 tcaagcttat tttcgcggag acgacgactt ttggagtgcg cacctaccgc gccgagcggc
    11881 gcgtactgcc gcgggaatgg gtgacggtgg agacggcatt cggcgacgtg cgcatgaagt
    11941 tgtcgcgagc gaacggaagg attctgcgcg cggcaccgga gtacgaggat tgccggaaac
    12001 tggcgacaga gcgcgacgtg ccgttgcaga gggtgatggc agaggccatg aggaattatg
    12061 aggagcaagt aaggaaagga ggttaaggaa gttaaggaag gtaataaagt taaggaacag
    12121 agggaagagg gaatagtggc gggaacgaag cctaagtttt atctgacgac gccgatctac
    12181 tatacgaacg gcctgccgca catcggccat acgtattcga cgatcgtttg cgacacaatc
    12241 cggcgctaca agcggatgcg gggatacgac gtggtgctca cgacggggac cgatgagcac
    12301 ggcgtgaacg tggagcgatc cgcgcggaag gcggggaagt cggaagcgga attcgtcaac
    12361 gaaatggcgg gcgaatggcg ccgcttgtgg gacgagctgg ggatccaggg c
//
LOCUS       DASYVO010000165         9460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_161139, whole genome shotgun sequence.
ACCESSION   DASYVO010000165 DASYVO010000000
VERSION     DASYVO010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9460
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161139"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VGR03_05520"
     CDS             <1..648
                     /locus_tag="VGR03_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV1993770.1"
                     /translation="DADSYEEKPGVTLMTLHSAKGLEFDQVFLAGLEEGIFPHSRSMN
                     ELDELEEERRLCYVGMTRARRSLTLTRALYRRIFGEEQRLRASLPSRFLAEIPSELVD
                     TIRGSLAEFGVARRYEPDPEYSYSPQEFARRVRSPHSRRADGESNRAARGLSSFGRKS
                     QSENPLIGQTVRHPTFGVGTIIAVEGDDDDRRLSVSFPGRGTKKFIERFARLEPA"
     gene            772..2307
                     /locus_tag="VGR03_05525"
     CDS             772..2307
                     /locus_tag="VGR03_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV1993771.1"
                     /translation="MGSELFRTKSIDQLIADSENPEKRLSKTLGWLSLTSLGIGAVIG
                     SGIFTVIGTAIAGQKLQASSILNAPLLEYLIHHSATFGRPGAGPAIAISFLLVAVVCA
                     FAALCYAELAAMIPIAGSAYTYTYATMGELIAWIIGWDLILEYAVSNMAVSVGFSAHI
                     VDLLDWFGVHPNPRWICPAYLPTGLPDFQGNLLIQPGWHLGFNFPAFIIVMLLSVVLV
                     RGIRESAKTNNVMVLLKIVAILVFISFAARFVNPSHWHPFAPNGWPGILTGGSIIFFT
                     YIGFDSVSTAAEETKNPQRDLPIGIIATLVVCTVLYIAVVVVLTGLVPWQSLLDDAAP
                     VVNTLRRLHLTTIRLIVLFGALMGMISSLLVFQLGQARVWFAMSRDGLLPRVFSRVHP
                     RFRTPDFSTWIAGIVVGIPSGIFDIGTLADLSNIGTLFAFALVAGGVLILRFREPDRH
                     RSFRAPLGLTAPIVTILTCLLLMAGLPIWNWLRFFVWLLLGLVFYYFYGRRYSTLRAQ
                     SAK"
     gene            2382..3179
                     /locus_tag="VGR03_05530"
     CDS             2382..3179
                     /locus_tag="VGR03_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993772.1"
                     /translation="MAASLPSRASAWGEKGSRLIVDKAVDTLPSEIRPFFEANRAFFI
                     RHAIDPIESAAKTPAEQKNRFLYLDRYGRFPFDALPRSYKSAVAKFGKPKIEANGILP
                     WQIGVYSEKLTNAMKAGKWEDARLFAALLASYVAEAHDPFNTTENHEGQLTGQHGVSD
                     RFGSNLVDRFSSFFPVRPNDAVFISDPTDRAFEACLSAHSWLETILLADRNARRGLPA
                     YTDEYYDRFYNQAAAILIRQVSDAATDVGSYWYTAWVNAGRPPLPQR"
     gene            3190..4209
                     /gene="add"
                     /locus_tag="VGR03_05535"
     CDS             3190..4209
                     /gene="add"
                     /locus_tag="VGR03_05535"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEV1993773.1"
                     /translation="MTVPRLPPEFLSLPKAELHLHLEGSIEPSLVCRLAARHGEIVSE
                     QEARRHYAYHGFAEFLEAFKWVTSYLRDPEDYALVTDALTASLLEQNVVYAELTISIG
                     VMHFRKQVPEANFEAIRRASEVSAGKGLRLRWIFDAVRQFGPEAAGKVALLARNYKKE
                     GVIAFGMGGDELSLPASDFRSTYDFVAAQGLHRLVHAGEIGGPQQVYDAIDLLGAERI
                     GHGIGAMHDPGLLETLAERRIPLEICPTSNLCTGALARQLGCDAPTLAQHPLPEFLRR
                     GIPVVLSTDDPAMFNTSLLAEYAQTAAMGLDEEERIRLAAQSFEHAFLPEEDKRALSA
                     RKPGL"
     gene            4255..4497
                     /gene="purS"
                     /locus_tag="VGR03_05540"
     CDS             4255..4497
                     /gene="purS"
                     /locus_tag="VGR03_05540"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEV1993774.1"
                     /translation="MKAHVWVMPKRTVLDPQGKTIQNALASLGYAQVKDVRQGKFFVL
                     DLNGWSREDARKHLERIAGEVLTNPVIEEYRFEIVD"
     gene            4539..6407
                     /gene="glmS"
                     /locus_tag="VGR03_05545"
     CDS             4539..6407
                     /gene="glmS"
                     /locus_tag="VGR03_05545"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV1993775.1"
                     /translation="MCGIVGYIGPKKVVPLILDGLRLLEYRGYDSAGIAVVNGNGKME
                     VRRASGKLRNLEEAIRKAPLDGTYGVGHTRWATHGRPTEENAHPHRDCTGQIVVVHNG
                     IIENYLELKEKLRREGHKFVTETDTEVVAHLVEKNMQAGVPLEEAVRLSLRELRGIYS
                     LVFLSANDPQKIVAARLGPPAVVGLGNGEYFVASDIPALLQHTREVFFLSDGDIAVLT
                     PQGVRVMDHDGCAVSRPSQHVTWDPIMAEKGGYKHFMQKEIFEQPRAVRDTLLGRISQ
                     DTGRIFLEEMEITEQQFREFQHVKLVACGTSWHSALAGKFMIERLSRIPAEVDYGSEF
                     RYRDPILDSRTLTICISQSGETADTLAAQREAKQKGSPTLAICNVMGSMVTREAAGTI
                     LTHAGPEIGVASTKTFTAQLTALLLLAIRLGEARGALSAESAKGLLQEFTRIPHKLEN
                     ILQQGEENGILDSLARQFFRHSDFLFLGRGIHYPIALEGALKLKEISYIHAEGYPAGE
                     MKHGPNALIDENLPVVVLATRDESDPDSMTLYEKSVSNIKEVKARDGIVISVVTAGDH
                     LAREASDHLIEIPAAPELLSPILEIVPLQLLAYHIAVRRGCDVDQPRNLAKSVTVE"
     gene            6509..7825
                     /locus_tag="VGR03_05550"
     CDS             6509..7825
                     /locus_tag="VGR03_05550"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEV1993776.1"
                     /translation="MCRCARLTLWTCGLLTLLLVAPRDLSASQGGTKAQLSKINIVGL
                     RSLSADLLLPETGLKPGDLAGRDEFQAAADRLAQLGLFSNITYEFRTQSDGLSVTFHA
                     EEAARVPAYFDNVPWFTDGELAEAIKKTVPFYDGTCPEGGSVLDRIAEAVRQLLQARQ
                     LQVAVEHEYIANPIGEGMVQVFRITGASLRIARIEFSDPLASSSRGLQVHVSELLGKP
                     YSRMQIDLFLAEHVRPVYLQKGYLRAKIGPPEIRLTGNPARPLPEMLPVFVPVTPGAV
                     YHWQGAQWSGNASLTISQLNDALALKSGETADGLAIQAALDRAQEEYGHRGYLEVQLD
                     PQPVYDDQAHTVSYRIAVAEGPQYRYGELVLTGASLAAEKRIRASWPMAAGELFDKLK
                     YEQFLTRLQSHGEEVLGDLPVHYAEVGHWLRTDALKKIADVFLDFK"
     gene            7891..8784
                     /gene="larE"
                     /locus_tag="VGR03_05555"
     CDS             7891..8784
                     /gene="larE"
                     /locus_tag="VGR03_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEV1993777.1"
                     /translation="MSTYVQIRGGAAPESPAKQKELLLLGRLAAAESLLVALSGGADS
                     AYLAWAAHRALGPRVLAITALSASFSAYDRERVEEFLRAVPLAHEFVETHELENPSYQ
                     ANQPDRCYYCKDELFSVMDELAAAREFTAVAYGVNADDTLDFRPGHRAASEHRVLAPL
                     LDACLTKPEIRLLSREAALPTWDRPASACLASRLPYGTRVTPERLSLVERGEAALREL
                     GFRQFRVRLHDEMARVEIAREELPRALSQEMALEIARRLRAAGFNYVTLDLEGYRQGS
                     LNESLPGYAKRANPSPNPDNH"
     gene            complement(8807..>9460)
                     /locus_tag="VGR03_05560"
     CDS             complement(8807..>9460)
                     /locus_tag="VGR03_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEV1993778.1"
                     /translation="AGGSILAAQRAMVDGFAANLGGGFHHAHPGYGEGFCAIHDVAVA
                     IRRLLADGRIEKAMVVDTDVHHGNGTAAIFRDDPRVFTLSIHQLNNYPAYKPSSSLDL
                     DMPDGAEDDEYLSALGPAVRRSLDSFQPQLVFYVGGADPYCEDQLGGLSLTMEGLRQR
                     DRLVFEETRRRGIPVAATLAGGYARQVDDTIRIHVNTILAAREVAGEFSSAAAERKT"
BASE COUNT         1725 a         3172 c         2632 g         1931 t
ORIGIN      
        1 gacgccgact cctatgagga gaaaccgggc gtcaccttga tgaccctgca cagcgccaaa
       61 ggtctcgaat tcgaccaagt cttcctcgcc gggctcgagg aaggcatctt tccccacagc
      121 cgatcaatga acgagctcga cgagcttgag gaagaacgcc gcctttgtta cgtgggcatg
      181 acccgcgcgc ggcgctccct cacgcttacc cgcgcgctct accgccggat cttcggcgag
      241 gagcagcgcc tgcgtgcctc cctaccctct cgtttcctcg ccgaaattcc atccgaattg
      301 gttgacacca tccgtggctc gttggccgaa ttcggcgtag cgcgccgcta cgagcccgac
      361 cccgagtatt cctactcccc gcaggaattc gcccgccggg tacgcagccc gcattcgcgc
      421 cgcgccgacg gcgagtcgaa cagggcggcg cgcgggcttt ccagtttcgg cagaaagagc
      481 caaagcgaaa acccgctcat cggccaaacg gtccgccacc ccacctttgg ggtgggcacg
      541 atcatcgccg tcgaggggga cgatgacgac cgccggctgt ccgtcagctt ccccgggcgc
      601 gggacaaaaa aattcatcga acgctttgct cggctcgagc cggcttgaga aaaccacccg
      661 gcggttgcac cgcggtggat tgtcggggta aagtggcccc cgcatccatc ttgcgaaggt
      721 cgagcgcgac ttccacgctc cggccgcgcc ggcagcgtac cgggagaacg agtgggatca
      781 gaactctttc gcaccaagag catcgatcaa ctaatcgcag actctgagaa tcccgaaaag
      841 aggctgagca agaccctggg gtggttgagc ctcacctcat tggggatcgg cgcggtcatc
      901 ggctccggta ttttcaccgt gatcggcaca gccattgctg gccaaaagct gcaagcctcg
      961 tccatcctga acgcgcccct gctggaatac ctgatccatc attcggcgac cttcggccgc
     1021 cccggcgcgg gccctgccat cgctatttcc ttccttctcg ttgccgtggt ctgcgctttc
     1081 gccgcgcttt gctatgccga attggccgcg atgatcccca tcgcgggcag cgcctacacc
     1141 tacacttacg ccacgatggg cgagctcatc gcctggatta tcgggtggga cctgatcctg
     1201 gaatacgcgg tcagcaacat ggccgtctcg gtcggatttt ccgcccacat cgtggatctc
     1261 ctcgactggt tcggagttca tcccaacccg cgatggattt gccctgctta cctccccact
     1321 ggccttccgg acttccaggg gaacttgctc attcaaccgg gctggcatct cggtttcaat
     1381 ttccccgcct tcatcatcgt catgctcttg tccgtggtgc tggtccgcgg gatccgcgaa
     1441 tcggccaaga cgaacaacgt gatggtcctc ctgaaaatcg tggccatcct ggttttcatc
     1501 tccttcgccg cgcgatttgt gaatccctcc cactggcatc ctttcgcacc caacggctgg
     1561 cccgggattc tgacgggcgg ctccatcatc ttcttcacct acatcgggtt cgattcggtt
     1621 tccaccgccg ccgaggagac caagaatccg cagcgcgatc tgcctatcgg gatcatcgcc
     1681 acgctcgtcg tctgcacggt gctctacatc gccgtggtcg ttgtcctcac cgggcttgtg
     1741 ccctggcaat cgctcttgga cgatgccgcc ccggtcgtca acacgcttcg ccgcttgcac
     1801 ctgaccacga tccgcctcat cgtcctcttc ggcgcgttga tgggcatgat ctcttctttg
     1861 ctggtcttcc agcttggaca ggcccgcgtc tggttcgcca tgtcgcggga tggcctcctg
     1921 ccgcgcgtct tcagccgcgt tcacccgcgc ttccgcactc cggacttctc cacctggatt
     1981 gccggaatcg tcgtgggaat tccctcaggc attttcgata tcggtactct ggccgatctt
     2041 tccaacatcg gcacgctctt cgccttcgcg ctcgttgcag gtggtgtttt gatcctccgc
     2101 ttccgcgaac cggaccgcca ccgcagtttc cgcgctccgc tcggtttgac cgcccccatc
     2161 gttaccatcc tgacctgtct tctcctgatg gccggtctcc cgatttggaa ctggttgcgt
     2221 ttcttcgtct ggctcttgtt ggggctcgtg ttttactatt tctatggacg gcgatacagc
     2281 acactccgag cccagtcggc aaagtgagga gcagttgtat gtcccaggcg tcttggcatc
     2341 gctttagaag ctctcttcct tcattcgcct tggcgctcgt cttggccgct tcccttccat
     2401 cgcgggcgtc tgcctggggt gaaaaaggca gccggttgat cgtagacaaa gccgttgaca
     2461 ccctcccctc ggagattcgc cccttctttg aagcaaatcg cgccttcttt attcggcacg
     2521 ccattgaccc gattgaaagt gccgcgaaga caccggccga gcaaaagaac cgcttcctct
     2581 atctcgaccg ctatggccgt ttccccttcg atgctttgcc ccgcagctac aaatcggcag
     2641 tcgcgaagtt cggcaagccc aagatcgagg ccaacggaat tctgccctgg cagatcggcg
     2701 tctacagcga gaaacttacg aatgccatga aagccggaaa atgggaggac gcacggctgt
     2761 ttgcggcgct gctggcgagc tacgtcgccg aagcccacga tccgttcaac acgaccgaga
     2821 atcacgaagg gcagttgacg ggccagcatg gggtcagcga ccgatttggt tcaaatctcg
     2881 ttgaccgctt ctcctccttc ttcccggtcc gccccaacga tgccgtgttc atcagcgatc
     2941 cgacggaccg cgcatttgaa gcctgtctca gcgcccacag ctggcttgaa acgatccttc
     3001 ttgccgaccg caatgcccgc cgcgggctgc ccgcttacac cgacgagtat tacgaccgtt
     3061 tctacaatca ggctgctgcc atcctcatcc ggcaggtctc cgatgcagcc accgatgtcg
     3121 gttcgtactg gtataccgcg tgggtgaacg ccggccgtcc cccgctgccg cagcgctgat
     3181 cgcctgcgaa tgaccgttcc tcgtcttccc ccggagtttc tctctttgcc gaaggcggag
     3241 ctgcacttgc acctggaggg ctccatcgag ccctccctgg tctgccggct cgctgcgcgt
     3301 cacggagaga tcgtttccga gcaagaggct cgccgccact atgcctacca cggctttgcg
     3361 gaattcctcg aggctttcaa gtgggtcaca tcctacttgc gcgacccgga agactatgcc
     3421 ctggtcacgg acgcgctgac agccagcctc ctcgaacaga acgtcgtcta tgccgaattg
     3481 acgatctcca tcggcgtcat gcacttccgc aaacaagtcc ccgaagccaa ctttgaggcc
     3541 atccgccgcg ccagcgaggt gagtgccgga aaaggtctcc gtctgcgctg gatattcgat
     3601 gctgtgcgcc aattcggccc ggaagcagcc ggaaaagttg ccctactcgc ccggaattac
     3661 aaaaaagagg gtgtcatcgc cttcggcatg ggcggcgacg agttgtccct gcctgcctcc
     3721 gatttccgtt ccacgtacga ttttgtcgcc gcgcagggcc ttcaccgtct ggttcatgcc
     3781 ggagagatcg gcggacctca gcaggtctac gacgcaatag atctcctggg tgcggagcgg
     3841 atcggccacg gaattggcgc aatgcatgat ccgggcttgc tggagaccct ggccgagcgc
     3901 cgcatcccac tcgaaatctg ccctacgagc aacctctgca ccggcgcatt ggcgcgtcaa
     3961 ctcgggtgcg acgcgccgac cctcgcccag caccctctgc cggaattcct gcgccggggc
     4021 atccctgtcg tcctctccac cgacgatccc gccatgttca acacctcttt gctcgccgaa
     4081 tatgcgcaga ccgccgccat gggcctggac gaagaggaac ggatacgcct cgccgcgcaa
     4141 agtttcgaac atgccttcct gccggaggaa gataagcgcg ctctgtctgc tcgaaaaccc
     4201 ggtctctgaa ggcacttcgc tgcttgcgaa ctctcccttt gctataattg ggttatgaaa
     4261 gcacacgttt gggtcatgcc gaagcgcacg gtgcttgatc cccaggggaa aacgattcag
     4321 aacgccctgg cttctctcgg ctacgcccag gtgaaagacg tccggcaagg aaaattcttt
     4381 gtgcttgatt tgaacggttg gagccgcgag gacgcccgaa aacacctgga gcgcatcgcg
     4441 ggcgaagttt tgaccaatcc ggtcatcgag gaataccgct tcgaaattgt cgattgatcg
     4501 ctgcgcgccg gccgcttcgg aataaggagt tccctctgat gtgcggaatt gtagggtaca
     4561 tcggcccgaa aaaagtggtt ccgttgatcc tcgacggcct ccggctcctc gagtatcgcg
     4621 gctatgactc ggcgggcatc gccgtcgtga acggcaacgg caagatggag gttcgccgcg
     4681 cctctggaaa gctgcgcaat ctcgaagagg ccatccggaa ggctcccttg gatggcacct
     4741 acggcgtagg acacacccgc tgggccaccc acggccgccc gaccgaggaa aatgcccacc
     4801 cgcaccggga ctgcaccgga cagatcgtcg tcgtccataa cggcatcatt gagaactatc
     4861 tcgagttgaa ggaaaaactt cggcgcgagg gccacaaatt cgtcacggaa acggacacgg
     4921 aagttgtcgc ccatctggtg gagaagaaca tgcaggcagg agtcccgttg gaggaggcgg
     4981 tgcgcctctc gctccgtgaa ttgaggggca tctactcgct ggtgtttctc tcggccaacg
     5041 atccacagaa aatcgtcgcg gcgaggttgg gccctcccgc cgtcgtgggc ctcggcaacg
     5101 gcgagtattt cgtggccagc gacatcccgg cgctgctcca gcacactcgg gaggtcttct
     5161 tcctttccga cggcgacatc gccgtcctca cacctcaggg ggtccgcgtg atggaccacg
     5221 acgggtgcgc ggtcagccga ccctcccagc acgtcacctg ggatcccatc atggccgaaa
     5281 agggcggcta caagcatttc atgcagaagg agatctttga gcagccccgc gccgttcgcg
     5341 ataccctcct cggacgcatc tcccaggaca cgggcaggat cttcctagaa gaaatggaga
     5401 tcacggagca gcaattccgc gaattccagc acgtgaagct cgtcgcttgc ggcacgagct
     5461 ggcattccgc cctggcggga aaattcatga tcgagcgcct ctcccggatc cccgcggaag
     5521 tcgattacgg cagcgagttc cgctatcgcg acccgattct cgattcgcgc accctgacaa
     5581 tttgcatcag ccagtcggga gaaaccgcgg ataccctggc cgcgcagcgc gaggccaagc
     5641 agaaaggctc cccgaccctc gccatttgca acgtcatggg ctcgatggtc acccgcgagg
     5701 ccgccggcac catcctcacc cacgccggac ctgaaatcgg cgtcgcctcc acgaaaacgt
     5761 tcaccgcaca gctcaccgcc ttgctcttgc ttgccatccg tctgggtgag gcccgcggcg
     5821 cattgtcggc cgaatccgca aagggcctcc ttcaggaatt cacgcgcatc ccccacaagc
     5881 tcgagaacat cctccaacag ggtgaagaga acggcattct tgattccctt gcccggcaat
     5941 tcttccggca ttcggatttt ctctttctcg gccgcgggat tcactaccct atcgccctcg
     6001 agggcgccct gaagctcaag gagatttcct acattcatgc cgagggttat cccgccgggg
     6061 agatgaagca cggcccgaac gccctgattg acgagaacct tcccgtcgtg gttctcgcga
     6121 cgcgcgacga atcggatccc gattccatga ccctctacga aaagagcgtt tcgaacatca
     6181 aggaagtcaa ggcgcgcgac ggcatcgtca tttccgtggt caccgccggc gatcatctcg
     6241 cccgcgaagc ctcggatcac ctcattgaga ttcccgcggc gccggagttg ctctctccca
     6301 ttctcgaaat tgttccgctc cagttgctcg cctatcacat tgccgttcgt cgcggctgcg
     6361 acgtcgatca accgcgcaac ctggcgaaat ccgtcaccgt cgaatgagca ttcacactcc
     6421 gcgcatcccg ctccggaaag cctcgtgcaa cccggcttca cacaaaacca tctcttcatc
     6481 cgtaggactt tcgtagaatg gacctgccat gtgtcgctgt gcccgcctca cactctggac
     6541 ctgcggcctg ttgacgcttc tgcttgtcgc cccgcgggat ctttcggcgt cccagggcgg
     6601 cacgaaggcg cagctctcga agatcaacat cgtcggcctc cgaagccttt ccgccgattt
     6661 actcctcccg gagactggct taaagcccgg cgatctggcc ggtcgcgacg agtttcaggc
     6721 tgccgcggat cgtttggccc agcttggcct gttctcaaac atcacctatg aatttcggac
     6781 tcagagcgac ggactctcgg tgacctttca cgccgaagag gccgcgcgcg ttccggccta
     6841 cttcgacaac gtgccgtggt tcaccgatgg ggaactcgct gaggccatta agaagacggt
     6901 tcccttctat gacgggacct gccccgaagg cggcagcgtt ctcgaccgca tcgccgaggc
     6961 cgtccgccag cttctccagg cgcgtcagct ccaggtcgcc gtcgaacacg agtacattgc
     7021 caacccgatc ggggaaggca tggtccaggt atttcgcatc accggggcct ccctgcggat
     7081 tgcgcgcatc gagttctctg acccgcttgc ctcctcttcc cgcggcctgc aggtccacgt
     7141 gtccgagctt ctcggcaaac cctattcgcg catgcagatc gatctgttcc ttgccgaaca
     7201 cgtgagaccg gtctatctgc agaagggata tctcagggca aaaatcgggc cgccggagat
     7261 acgcctcacc ggaaaccctg cccggccact accggaaatg cttcctgtgt ttgtgcccgt
     7321 aaccccagga gcggtctacc actggcaggg cgcacagtgg tcggggaatg cctccttgac
     7381 gatttcccaa ttgaacgacg ctctcgcttt gaaatccgga gagaccgcgg acgggctggc
     7441 tatccaggca gcgctcgacc gcgcgcagga agaatacggc catcggggtt atcttgaagt
     7501 ccaactcgac ccgcagccgg tctacgacga tcaggcgcac accgtttcct atcgcattgc
     7561 cgtggcggaa ggccctcaat accgctatgg cgagctcgtc ctcaccggcg cttccctcgc
     7621 cgccgaaaag cgcatccgcg catcctggcc gatggcggcc ggagaactct tcgacaagct
     7681 gaagtacgaa caattcctca ccaggctcca gtcgcacgga gaggaagtac tcggcgattt
     7741 gcccgtccac tacgccgaag tcggtcattg gcttcgcacg gacgcgctta agaaaatcgc
     7801 cgacgtcttt ctggatttca aataacgccc ctcgcggttc cttcttcaac tcgcttcctt
     7861 gcatgtttcc ttgcgctata ctgtccggcg atgagtacct atgttcagat tcgcggaggc
     7921 gccgcgccgg aatcgccggc caagcagaag gagcttctgc tcctcggccg gcttgcggca
     7981 gccgaatcgc tcctggttgc gctttcgggc ggcgccgatt ccgcttatct cgcgtgggcg
     8041 gcgcatcgtg ccctggggcc gcgcgttctt gccatcaccg ctctctccgc gagcttttcc
     8101 gcctacgacc gggagcgcgt cgaagaattc ttgcgcgccg tgccgctcgc gcacgagttc
     8161 gttgagaccc acgagctcga gaatccttcc tatcaagcca atcagccgga ccgctgctac
     8221 tactgcaaag atgagctctt ctccgtcatg gatgaattgg ctgcagccag agagtttacg
     8281 gccgtcgcgt atggcgtgaa cgccgacgac acgctcgact ttcgtcccgg ccatcgtgcc
     8341 gcttccgagc atcgcgtcct cgcgccgctg ctcgatgcct gcctcaccaa gccggaaatt
     8401 cgcctgctct cgagggaggc ggctctgccg acctgggacc gtcccgcttc cgcttgcctg
     8461 gcctcccgct tgccctacgg tactcgcgtc accccggagc ggctctctct tgtcgaacga
     8521 ggagaggcgg ctctgcgcga gttgggattc cgccagtttc gcgtccgttt gcatgatgag
     8581 atggcgcgtg tggagatcgc ccgcgaagag ctgcctcgcg cgctgtccca ggaaatggcc
     8641 ctcgaaatcg cgcgccggct tcgagcggct ggcttcaact acgttacgct cgatctcgaa
     8701 ggctatcggc agggctctct aaacgaatcg ttgccgggct acgcaaagcg agcgaatccc
     8761 tctccgaacc cggacaacca ttgaagcgat ccagctcgca gccggactaa gtttttcgtt
     8821 ccgccgcagc ggacgaaaat tctccggcta cttcgcgcgc cgcaagaatt gtatttacgt
     8881 ggatccgtat cgtgtcatcc acttggcgcg cgtagcctcc cgccagggtc gcagccacgg
     8941 gaatgcctct gcggcgagtc tcttcaaaga ccagccggtc gcgctgccgc aggccctcca
     9001 tcgtcagcga gagaccgccg agttgatctt cgcagtacgg atccgcccca ccaacgtaaa
     9061 acacgagctg cggctgaaaa ctgtccagcg agcggcgaac ggcggggcca agcgcggaga
     9121 gatactcgtc gtcttccgcc ccgtccggca tgtccaggtc caggctcgaa gacggcttgt
     9181 acgccggata gttgttgagc tggtgaatcg aaagtgtgaa gactctcgga tcgtcccgga
     9241 aaatcgcggc cgtgccgttg ccgtggtgga catccgtgtc cacgaccatc gctttctcga
     9301 tgcgtccgtc ggccagcagc cgccggatcg ccacggccac atcgtgtatc gcgcaaaaac
     9361 cctcgccgta gcccggatgc gcgtgatgaa accccccgcc gaggttcgcc gcaaatccat
     9421 ccaccatcgc ccgctgcgcc gccagaatcg agccgcccgc
//
LOCUS       DASYVO010000166        18401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_214143, whole genome shotgun sequence.
ACCESSION   DASYVO010000166 DASYVO010000000
VERSION     DASYVO010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 18401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18401
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214143"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VGR03_05565"
     CDS             complement(<1..416)
                     /locus_tag="VGR03_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993779.1"
                     /translation="MPFYAHPTPQISPGDIFPSIPFPVVVHPLKIARNESYTPPAGRG
                     PAEFRRIYTIPDDEARITNLTIATTQGEETLSKTRRTMALFLTWGSEVESTELRIVEN
                     GRVGKRSWLAAPVYSLQEVPAGNTEIDPDTNEQVPLR"
     gene            complement(422..676)
                     /locus_tag="VGR03_05570"
     CDS             complement(422..676)
                     /locus_tag="VGR03_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993780.1"
                     /translation="MLVIAFSAATAAVLLCQYAVPAPTIGTKGLLLVGESVSVGSGVN
                     ARSEEPRAQHHVAPFKLISRRQSEPAPTSKYVSLDLEDFQ"
     gene            complement(810..1397)
                     /locus_tag="VGR03_05575"
     CDS             complement(810..1397)
                     /locus_tag="VGR03_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993781.1"
                     /translation="MLYTFPKGEGGPIEIRIDFGLVTAPLNYYYADSLHLALDENQRM
                     VVLSFGRRNETTNKFANRVDVAMPKKSLFDQFWKSSAGVQSTLDKFLEATGAAPKLQP
                     ISSPEPQPTTFFANAIFVALGEGESTLDFYHLPPREVHLAKTLKANIQIQPIVRVIMS
                     SVLTKHFFETLRPHAEQGLTLQTVEERSRRARTPH"
     gene            2146..2490
                     /locus_tag="VGR03_05580"
     CDS             2146..2490
                     /locus_tag="VGR03_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993782.1"
                     /translation="MLCRPPHSSLCPFHAREELQILEAGKIAAAFASVTGGFYTFTDV
                     NQALGTLFRFVAANRIPPRSAVTLAYLGQLLLQSIPGVEREANLVRGRDGWRNLLTFV
                     LNPRKPKDGLVQ"
     gene            3021..4469
                     /gene="der"
                     /locus_tag="VGR03_05585"
     CDS             3021..4469
                     /gene="der"
                     /locus_tag="VGR03_05585"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV1993783.1"
                     /translation="MPENPPSLVPSLVIVGRPNVGKSALFNRLTGTRRSIVTNEPGIT
                     RDRIYGRVEWRGRSIEVVDTGGIIPDDKALIPHEIFRQARVAIEKAGLLVLVVDTQAG
                     LTPMDAEIGRLLRATGKPFVVAANKVDVPRLEVQAAVFHALGAPVFPITAEHGAGVDD
                     LLDYALETLSPAPAQEDKPTEAPVEVAIIGRPNVGKSTLLNRLVGEERAIVSPEPGTT
                     RDSVDTEVRVEGRLYRFVDTAGIRKKGKTKLVAEKLSVVMARRSLERADVALLIADAE
                     HGVTKGDATIAGYAEQSGRSVIIVMNKWDLAVEAAREAAERSAAAAAADSGSRRPAAR
                     NLPPAFEKGKLLLEYETLVRKKLKFLSYAPIVFLSAKTGDRTDKLYPLIQQVAAARRR
                     IPTPELNRWLAGVDLSRGTSPKARTVKIFYITQASASPPTFILFTNQMRPLHFSYERF
                     LENQLRAKFDFTGTPVRFLQRTRKRDKKGARR"
     gene            complement(4730..5107)
                     /locus_tag="VGR03_05590"
     CDS             complement(4730..5107)
                     /locus_tag="VGR03_05590"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEV1993784.1"
                     /translation="MSPNSRGTKWNDPRFGQRIRSLEYISVHYEGSTEEIASRLPDVS
                     PRGMFISTSREFPAGSVLILKFRLAFTGAEVRTRGEVRYCLSGVGVGVEFVGISPQAV
                     RKIKKEIELRKRRRAGAHRKKKK"
     gene            complement(5104..6981)
                     /locus_tag="VGR03_05595"
     CDS             complement(5104..6981)
                     /locus_tag="VGR03_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEV1993785.1"
                     /translation="MKKKRLGEVLRERGHVSAAELEIALQDQQSKLGRLGELMLERGF
                     VSKKDLVSALREVSPIPYLDCTTIQIAPAVIRLIPPALAKRCFAVPVEVRDSRLVVAM
                     AEPQNLHILDELRFQTGRDIVPRLAFRAEINAAVEKYYGKSVPTDDVPAEEYAPPPQD
                     KGIEFVSWNEQQRNIDAIEEMQSELSQKSKATPAVLLVASMIKAAVDKGASDIHVEPQ
                     SEDTAIRFRVDGMLREYLRVPRNLQNTVASRIKILADMDISERRASQDGRFLVRLAGR
                     RIDLRISTLPTQYGEKVVMRLLESEAPSQDFEKLGFPVPIADALKRMLVLPQGMILVT
                     GPTGSGKSTTLYSSLTFVRKPSLNIITVEDPVEYALPGLNQVQVNTKAGVTFATCLRS
                     ILRQDPNVIMIGEIRDKETAEIATKAAQTGHLVLSTLHTNDSISAVTRLLDLGMAPFQ
                     LATSLTGIIAQRLIRKLCSCSKEIPATPEFTVQLLQAGITDPPALQHVVNGCDKCDQT
                     GYKGRIGIYEMLATDDAIRSAIRDGGRHDKILPLARRNGMKLMYEYALEHVRDGLTTM
                     EEVLRVVPIERTTTTTLKCEACQRELAGASLFCPFCGAKGPGAVKSQQDSVTVPGMVK
                     N"
     gene            7280..7666
                     /locus_tag="VGR03_05600"
     CDS             7280..7666
                     /locus_tag="VGR03_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1993786.1"
                     /translation="MPGNSQSTQLAIEKALYRIGEVSRLTGTKAFVLRYWETEFPMLQ
                     PVKSPSGHRVYRKQDVEMVLKIKRLLYDEGFTIAGARRHLRELNGNAAREPAGSAEGP
                     AQLLSRKTLLDLRDALRGFLTLLERR"
     gene            7714..7790
                     /locus_tag="VGR03_05605"
     tRNA            7714..7790
                     /locus_tag="VGR03_05605"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7748..7750,aa:Pro,seq:ggg)
     gene            7897..8091
                     /locus_tag="VGR03_05610"
     CDS             7897..8091
                     /locus_tag="VGR03_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993787.1"
                     /translation="MTIQTNPEMFSPKLPVHTVTHIDRAAGVVTLSGSDGSSQQVRLD
                     SWGSFEEAQPLIGSKVQLLP"
     gene            complement(8119..8862)
                     /locus_tag="VGR03_05615"
     CDS             complement(8119..8862)
                     /locus_tag="VGR03_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993788.1"
                     /translation="MNLADYEQPKEWWWVVECSDYLRKLHPGTPRYAVIPISHLNQYS
                     NIQCRVRYSTETTANAAAIAAEGGKETLFAWEVVWWSASYRAEHPETSRFEAVCQNDI
                     RNFWSLGAKTVCSGMSRPDAEHEAARRSSLPELWCVITNEESRTNRQEPYTVKSFDGL
                     SFTEKEDKVRATCDSETAAWQAIPGIVEEDRQREAAYEQQQREWRDTQRRNESTGRVL
                     RFAVMVASIAGAIWLLVTVVHYFWVHPLF"
     gene            complement(9078..9884)
                     /locus_tag="VGR03_05620"
     CDS             complement(9078..9884)
                     /locus_tag="VGR03_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1993789.1"
                     /translation="MYPRFEQFVKEKQYLQNVSIATLSWYRHAAKWLPCESPDADQLK
                     QMVMAMREAGLKPTGANAAIRAVNVYLKWSGSQHKVPQLKEPEFVLPTFSDIQIKLLV
                     TSKPHDRLHLLVLVLLDSGCRISEALGLQWSDVDWDNCLLTLHGKGRKDRLVPFSHEL
                     RKALWRQRGADPCPFLWRFDRSSALRRVKTLCRRLGFAPPRRTLHAFRHTFALNYVRR
                     GGSVFHLQKALGHSDLEMSRRYVSLQTGDMVAAHRSLLCDFSVPLASNRL"
     gene            9919..9995
                     /locus_tag="VGR03_05625"
     tRNA            9919..9995
                     /locus_tag="VGR03_05625"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9953..9955,aa:Pro,seq:ggg)
     gene            10089..10868
                     /gene="surE"
                     /locus_tag="VGR03_05630"
     CDS             10089..10868
                     /gene="surE"
                     /locus_tag="VGR03_05630"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEV1993790.1"
                     /translation="MPMTLNKPHILLTNDDGFHAPGLHALAAALEDWATLSVVAPKRE
                     QSGAAQSLTLRTPIVCHPMAERQWAVDGTPADCVIVALHKLLPKPPDLVLSGINLGGN
                     LGENVYYSGTVGAAREAALHHIPSCAISLCAKHAPARFDDAARIARAMAELILREGLP
                     DQVLLNVNVPDSWEGGVKFTRQSRKITRNQLQEGVDPRGRAYYWLFEQRITKDVEPDT
                     DYAAIFTNALSITPLHLDPTHTESLNHLSRWAKPLAAAFQR"
     gene            10995..11285
                     /locus_tag="VGR03_05635"
     CDS             10995..11285
                     /locus_tag="VGR03_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993791.1"
                     /translation="MEALEPMESKAMNINLTPEQQKIVNDELKAGHFRTVAEVIAEAL
                     QVLREKERSADVGSPNGAQREAVREMLAFVEKNRVRLEGVSVKELIHEGHRL"
     gene            11282..11698
                     /locus_tag="VGR03_05640"
     CDS             11282..11698
                     /locus_tag="VGR03_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEV1993792.1"
                     /translation="MSDFVLDASLALQWFLEDEADRKYSLAVLASLSENRAVVPLLWF
                     YEVGNGLLMAYRRKRITLDQIDGFLTRLKALPIDAAQQTPSEILELPTLAQTYSLTNY
                     DAAYLALAMRSNSSLATTDIDLRKAVSAAGLRIVTA"
     gene            11970..13184
                     /gene="solA"
                     /locus_tag="VGR03_05645"
     CDS             11970..13184
                     /gene="solA"
                     /locus_tag="VGR03_05645"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HEV1993793.1"
                     /translation="MGRLLSKGGLPGTRAGGRAAMGGVKTYDAAVVGAGVFGSWTAWH
                     LAQAGKKVLLLDAYGPANSRASSAGESRIIRSGYGPDALYTRWAVRSLVLWKEFFAQT
                     GIGLFQNCGVLWLAREKDFYPKQTAETLTKMGVPFERLERTALQRRFPQFNLDDVDWG
                     LFEPEAGALLARRAVQAVVVAAVRKGVTYEQAAVNPPAAKGRLERIETSSGERLSAGE
                     FVFACGPWLGKVFPSVLGERIFPSRQEVFFFGVPAGNGQFASPAMPAWICLADEVYGL
                     PDLESRGIKIALDRHGERVDPDSQARIVSKETTEWMRSYVDRRFPALRGAPVVETRVC
                     QYENTSNGDFLIDRHPEMENVWLVGGGSGHGFKHGPALGEYVAGRLAGGAAEEPRFSL
                     ATKRTVQKRAVY"
     gene            complement(13245..14438)
                     /locus_tag="VGR03_05650"
     CDS             complement(13245..14438)
                     /locus_tag="VGR03_05650"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEV1993794.1"
                     /translation="MWLRHVPIKTDPVLHGYLQVITGLLALVFVAVALVRFQGSQDRI
                     SLILGSGFLLSGAVLAASSVLFFQLLRDVPLRFFWAPVAWWVSRMLLGVLFVVAILVD
                     RFLPRSRHPRREIAGALLAVVTLTYLISAGLRAHPPETSAHPGAVFPSPLQLIPALFF
                     LAALIGYRRRISRSDSAFDRMVYAAAWLNLAAQLAASQSVHLLDAPFALAQGLQVTGF
                     AAALGGALLDNARLFEKVRQMATSDPVTSLANYRRLLDVLESETERTNRTGRPFAVLL
                     LDLDGLKKINDAYGHLVGTRALCRLAEILRVHCRAIDTAARYGGDEFALVLPETPESD
                     AKRVANRIREVLARDHEKPPISASIGISIYCGDGERIEKLLSQADEKLYLDKSRRARR
                     RPHLG"
     gene            14891..15820
                     /locus_tag="VGR03_05655"
     CDS             14891..15820
                     /locus_tag="VGR03_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEV1993795.1"
                     /translation="MSLMRSMLLAASQSRWLREHATRYRFIRRSVNRFMPGESLDDAL
                     AAAQALQSKKLDAVLTQLGENVKEASEAQKVAAHYAEVLDRVHATGLRAEISVKLTQL
                     GLDLSPDLCFEHLKGIVARERKEATVWIDMETSPYVDATLNLYRRALHSYPNIGVCLQ
                     AYLYRTKEDLASLLPLRPSIRLVKGAYNEPPHVAFPKKRDMDENYFALAQELLRAKKE
                     NRCARAAFATHDVALIRRIFDAASREGFSERDLEVQMLYGIQRAEQERLAGEGCRTGV
                     LVSYGSYWYPWFVRRLAERPANLWFLARNIFAG"
     gene            complement(15822..16505)
                     /locus_tag="VGR03_05660"
     CDS             complement(15822..16505)
                     /locus_tag="VGR03_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993796.1"
                     /translation="MRKWGILITVFYAVIVLGLLVPGAVFLYGDKSSSWREFFGGVRG
                     VYLEWLSWIPIGIVLAGQALLLFLSVDASRKRLKPRAHILVSSSLTAMLLALLTFAAL
                     FSLAAAIHGDKLDNSPFFNSEAKVLAMWAFLWLFWGILFYLYFRNSTELLTRAVSWLL
                     KGSVLELLVAVPCHVIVRRRHDCSAPAATSFGIAAGIAVMLLSFGPSVLLLYKKRLDA
                     YPTSGSATE"
     gene            complement(16830..17885)
                     /locus_tag="VGR03_05665"
     CDS             complement(16830..17885)
                     /locus_tag="VGR03_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993797.1"
                     /translation="MNAGSTVELNNQIYERSLLKQPSLLSRILSSAWTIFLVFSLISF
                     ALLPFSNHFFGGLLAARGYPTWWVTVPLIVLRAIIVVTVIGYVNHRFVEHVGWMTRID
                     DHVRPFFGKILARALGEFVRDAQRDHKIHHWWQYPFGELFVRNVKYVGAVQGFSWEWY
                     IPGFIVGAAFIAINGLNWGTALFVATVYVYAQWVVSVVHHRFHEDVATSHPRWARSRY
                     FQWLFKLHILHHYDEDHNHDIVLPIMDFLTGRLLWPRFHVEEIQGLRDEKIICISQIR
                     NWRYVLLHATPAQRASFVTDATRPGRGPATVQMVRNLLSLLEDRLTLHPDDAQAQLLK
                     QRAECVLTELASAAQAA"
     gene            18219..>18401
                     /locus_tag="VGR03_05670"
     CDS             18219..>18401
                     /locus_tag="VGR03_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993798.1"
                     /translation="MKGILVRSLAVLLSLILLPAPDWATCGGGGGGGMGGMGGGGTSD
                     QKVYTVPWKLIKPGDPP"
BASE COUNT         3730 a         5626 c         5369 g         3676 t
ORIGIN      
        1 cgcaggggca cctgttcatt cgtatcagga tcgatttctg tgtttccggc gggaacttcc
       61 tggaggctgt acacgggcgc tgccagccag cttctctttc caaccctgcc attctccaca
      121 atcctgagtt ctgtggattc aacttcgctg ccccatgtca ggaagagcgc cattgttctt
      181 cgcgtcttgg agagagtttc ttcgccctgg gtcgtcgcta tcgtgagatt ggtgatacgg
      241 gcttcgtcgt ccgggatggt gtagatcctg cggaattccg ctggtcctcg acctgccggt
      301 ggcgtgtagc tttcgtttcg cgcgattttc aacggatgga ctacgaccgg aaaggggata
      361 gaagggaaaa tatcgccggg tgaaatttgc ggcgttggat gcgcgtagaa cggcatcgcc
      421 cttattggaa gtcttcgaga tcgagggaga cgtacttgct cgtgggggcg ggctcgctct
      481 gtctccttga aatcagctta aagggtgcca catggtgttg agctcgaggc tcttctgagc
      541 gggcattgac gcctgacccc actgacacgg attcgccgac cagaagaagg cctttcgttc
      601 cgatggtggg ggcaggtacg gcatactggc agagtaatac tgctgctgtg gccgccgaaa
      661 aggcgatcac cagcatttgg gctgcctcct cctgcggctt cgcgctacgg cgggccgtgc
      721 actcggcggt ctgcactcgt ggtgactgga cgcggaggaa gggtccctca gtcgggatct
      781 gtagcgggta gtacatatct tctgccgtgt caatgaggtg tacgggcacg tcggctcctt
      841 tcttcaaccg tctggagggt gagtccttgc tccgcgtgcg gtcttaaggt ctcgaaaaag
      901 tgtttcgtca ggacgctcga cataatcacg cgaacgattg gctgaatctg gatgtttgcc
      961 ttcaacgtct tggcaagatg cacctcgcgc ggaggcaggt gataaaaatc gagtgtagac
     1021 tcaccttcgc ctagagccac gaagattgcg tttgcgaaga aagtcgtggg ctgaggttcc
     1081 ggtgaggaga ttggttggag cttcggagcc gcacccgtcg cttcaagaaa tttgtcgagt
     1141 gtgctttgaa cccccgccga actcttccag aactgatcga atagcgattt cttcggcatg
     1201 gccacgtcga cgcggttcgc gaacttgttc gtggtttcgt tgcgacggcc gaatgatagg
     1261 actaccatac gttggttttc gtcgagagca aggtgcagtg agtcagcata gtagtaattg
     1321 agtggtgccg taaccaagcc aaaatcgatt ctgatctcga tagggccgcc ctcacccttg
     1381 gggaaagtat aaagcactcc cttcgtcccg aagatttttg gcggcttcgc tcccttcttc
     1441 gccttggtga tcttagcgga cactctgtgc gtaactcccc tgaatatgcg attccccgga
     1501 gaccggaagc gttaccttgt gttggggaag gttactcgcg taccgtgcat ggcgcaaagt
     1561 actttcgtac tggggtaggc aaagagaaag tcgtcattcg ccgcattttc gccccagccc
     1621 ttgggtgtgg tatgggatgc ctgttcggat ttcgggagcg tttcagtgtt ctcccatgat
     1681 caggataccc caacgggcta agacttggaa tctggatcaa tcgagtgggg aaaatttgag
     1741 ggtgagtggg catggaacgc ggttggtcgg aattctgccg ggtgccccag gtgccgttcc
     1801 cgcacctggg atttttgagc tgtgccggtc ccacccactc gtccgctccg gcgggatctc
     1861 tggcccctcc ttccagaatc aaggcccctt gtccccaacc tcttcccctt caacgactta
     1921 caaaaaatac ttgacaatga ctgtactttt gcggtaacat atcttgtgag atcggcccta
     1981 caggtcgctt tcctgcttcc gctcgtaccc tcactgcttc actacttcgt ttcttcatcc
     2041 taaggagctc tcgtgaaacc ccctaactcc cctgtttcag acaagatagc tgcgcccgct
     2101 tcgcctcgat gcgctttctc cttctctgac ggacgccgct gccgcatgct ctgccgtcct
     2161 ccccattcct ccctctgccc cttccatgcc cgcgaggagc tgcaaatcct cgaagctgga
     2221 aaaattgccg ctgcctttgc cagcgtgacc ggagggtttt acaccttcac tgacgtcaac
     2281 caggccctcg gcacgctctt tcgcttcgtc gccgccaacc gcattcctcc ccgctccgcc
     2341 gtgaccctcg cctatctcgg ccagctcttg ctccagagca tccctggcgt cgagcgcgag
     2401 gccaaccttg tgcgcggcag ggatggctgg agaaatctcc tcaccttcgt cctcaacccg
     2461 cgcaaaccca aagatggcct cgtccaatga tgcgcactcc attgacctcc ctcagtagca
     2521 tagacggaac tcgtcctgag caacgccgaa ggggtctgtg cgccttgcgt ttgttcaggt
     2581 ccgtctctgc tcaacccctg ccgaatcaac acttacagac ccccctcgta agtgcggaat
     2641 cgaaaggact tacagcgcga aaattcctct cccaactccc ctgttatcaa cacttacgag
     2701 cacctctcgt aactgcggaa aacacaagac ttataacccc tttggattcc gcacttccaa
     2761 gagatgcctc tcgcaagtcc tttagaatcc gcacttccag aaaatctggg gtggggggta
     2821 gtattgccat tcccaaccaa aatctctttc ttctcagcgc gccgatcaca atcgacggct
     2881 ccggaccgag aagtgacatc catcccgcct cacccttcga ctttcaactt tcaactttga
     2941 actcgtacct tcatcctccg acttcggact cgtacctttc cccggcctcg ccgaatgctg
     3001 tacattgcag gattgctccc atgccggaaa accctccatc gctggtaccc tcactggtca
     3061 ttgtcgggcg gccgaacgtc ggcaagtccg cgctcttcaa ccgcctgacc ggcacgcggc
     3121 gctccatcgt gacgaacgag ccgggcatca cccgcgaccg catctacggc cgtgtggagt
     3181 ggcgcggccg ctccatcgaa gtcgtggata ccggcggcat catccccgac gacaaggcgc
     3241 tgattccgca cgagatcttc cgtcaggcgc gcgtggccat tgagaaagcc ggcctgctcg
     3301 tcctcgtcgt ggatacccag gcgggcctta cgccgatgga tgcggagatc gggcgcctgc
     3361 tgcgcgccac gggaaaaccg ttcgttgtgg cggcgaacaa agtggatgtg ccccgcctcg
     3421 aagtccaagc cgcagtgttc cacgccctgg gcgcgccggt ctttcccatc accgcggagc
     3481 acggcgccgg cgtggacgac ctcctcgatt acgcgctcga aactctctcc ccggccccgg
     3541 cccaagaaga caagcccacg gaagcaccgg tcgaggtcgc catcatcggc cggcccaacg
     3601 tcggcaagtc taccctgctg aaccggctgg tgggcgagga gcgcgccatc gtctcgcccg
     3661 agccgggcac cacgcgcgac agtgtggata cggaggtccg tgtcgaaggc cggctctacc
     3721 gctttgtgga cacggcgggc atccgcaaga agggcaagac gaagctcgtg gcggaaaaac
     3781 tcagcgtggt gatggcgcgg cgcagcctgg agcgcgccga cgtcgctctt ctcatcgcgg
     3841 acgccgaaca tggcgtcacc aagggcgacg cgacgatcgc cggctatgcg gagcagtccg
     3901 gccgctccgt catcatcgtg atgaacaaat gggacctcgc cgtggaggcc gcgcgggagg
     3961 ctgcggaacg ttcggccgcc gcggctgccg cagactcggg gtcacgccgg cccgccgcaa
     4021 gaaatcttcc gcccgcgttc gagaagggca agctcctgct cgaatacgaa acgctggtcc
     4081 gcaagaaact gaaattcctg agctacgcgc cgatcgtgtt tctctccgcc aagacgggag
     4141 accggaccga caagctctac ccgctgatcc agcaggtcgc cgcagcgcgc cgccgcatcc
     4201 ccacgccaga gttgaatcgc tggctggccg gcgtggacct cagccgggga accagtccca
     4261 aggcgcgcac ggtgaaaatt ttctacatca cgcaggcttc cgcctcgcct ccgacgttta
     4321 tcctcttcac aaaccagatg cggccgctgc atttcagcta cgagcgtttc cttgagaatc
     4381 agctccgcgc gaagttcgac tttaccggca cgcccgtccg cttcttgcag cgcacgcgca
     4441 agcgcgataa gaaaggcgcc cggcgatgac gatgtgccct tccgctttca gcgccgcggc
     4501 gcgcgaatac tcgttccgct tgccctcggg ttccgaaccg cggcttcccg ggcaggatcg
     4561 agaaggaact gcgaaaagat ccgagcgtca cgggtgccaa ggggatttac gggcagaaac
     4621 ctttgattcg caccggctcc agccaaggga aggacgactg tgccgcggca ggggcaaagc
     4681 gcgggacttc tcagcaagcc cggttaaacc tgggagccga tgggagcgcc tatttctttt
     4741 tcttgcggtg cgcccccgcg cgtctgcgtt tgcgcaactc tatctctttt ttgattttcc
     4801 ggaccgcctg cggcgatatc ccgacgaatt ccactcccac tccgactccc gacaggcaat
     4861 accgaacctc gcctcgggtg cggacctccg ccccggtgaa cgccagccga aacttcaaga
     4921 tcagcaccga ccctgcgggg aattcccggc tcgtcgagat gaacatcccc cgcgggctga
     4981 cgtccggcag ccgagaggcg atctcttcgg tgctaccttc atagtggacg gagatatatt
     5041 ccaggctgcg aatccgctgg ccaaaccttg gatcattcca ttttgttccg cgagagttgg
     5101 ggctcaattc ttcaccattc ccggcaccgt cactgaatcc tgctgcgatt tcacagcccc
     5161 agggcccttc gctccgcaga aggggcagaa caaggaagcg ccggctaact cccgctggca
     5221 cgcttcgcac ttcaacgtcg tggtcgtggt acgctcgatc ggcacgactc gcagaacttc
     5281 ctccatcgtt gtcagcccat cccgcacatg ttcgagcgcg tattcataca tcagcttcat
     5341 cccgttgcgc cgcgcgagcg gcaggatttt atcgtggcgt ccgccgtcgc ggatggcgct
     5401 gcggatcgca tcatcggtag ccagcatttc gtatatgccg atgcgcccct tgtatccggt
     5461 ctggtcgcac ttatcgcaac cgttcacgac gtgttgcaaa gccggcgggt ccgtgattcc
     5521 ggcctgcagc agttgcacgg taaattccgg ggttgcagga atttctttgg agcaggagca
     5581 cagcttccgg atcagccttt gcgcgatgat cccggtcagt gatgttgcca gctgaaatgg
     5641 cgccatgccc aggtccagca agcgcgtcac cgcggagatg ctgtcgtttg tgtgcagcgt
     5701 cgaaagcacc aagtgtcccg tctgcgcggc cttggtggcg atctccgcgg tttccttgtc
     5761 tcgaatttcg ccgatcatga ttacgttcgg gtcctgccgc aaaatcgaac gcaggcaggt
     5821 cgcgaaggtc actcccgctt tcgtgttcac ctgcacctga ttcaatcccg gaagtgcgta
     5881 ttccacggga tcttccacgg tgatgatgtt taaggacggc ttgcgaacaa acgtgagcga
     5941 ggaataaagc gtcgtgcttt ttcccgaacc ggtcggcccg gtgacaagaa tcatgccttg
     6001 cggcagaacg agcatgcgct tcaatgcgtc agcgatggga accggaaatc caagcttctc
     6061 aaaatcctgt gatggcgcct ccgattcgag caggcgcatc accaccttct cgccgtattg
     6121 ggtcgggagc gtggagattc gcaggtcgat ccggcgtccg gcaagcctga ccaagaagcg
     6181 gccgtcctga gacgcgcgcc tctctgaaat gtccatgtcg gcaagaatct tgatgcgcga
     6241 agccacggta ttctgcagat tcctcgggac gcgcaggtac tcgcggagca tgccgtccac
     6301 gcggaaccgg atcgcggtat cctccgactg cggctcgaca tggatgtcgc tcgccccctt
     6361 gtccacagcc gctttgatca tcgaagccac caaaagcacc gccggcgtgg ctttcgattt
     6421 ctgactcagc tccgactgca tctcttcaat ggcatcgata tttcgctgct gctcgttcca
     6481 ggagacaaac tctatgccct tgtcttgcgg cgggggagcg tactcctctg ccgggacgtc
     6541 gtctgttggt accgattttc cataatattt ctccacggcc gcattgatct cggcccgaaa
     6601 cgccagccgc ggaacaatat ccctccccgt ctggaaccgc agctcgtcga ggatgtgcaa
     6661 gttctgcggc tcggccatcg cgaccacaag tctggagtcc cggacttcaa ccggcacggc
     6721 aaagcagcgc ttggccaagg ccggcggaat gagcctgatg accgccgggg cgatttgaat
     6781 ggttgtgcag tccaggtagg gaatcggcga cacttcccgg agtgccgaga ccaggtcttt
     6841 cttggaaacg aagccacgtt ccagcatcaa ctcgcccaag cggccgagct tgctttgttg
     6901 gtcttggagt gcgatctcca gttcggccgc ggaaacatgg ccgcgttcgc ggagaacttc
     6961 ccctaaccgc ttcttcttca tcgtcgtccc agtcttactc aggcaacaac aaaaaaccaa
     7021 agaaataagc caaaaccgaa gagagttcgc taaattttca ttctatcaca ggaggaaaaa
     7081 tgagcgattc gcctgtcgct ttcgttaccc ccgcaaactg gttgatccgt gtcgtgaaca
     7141 ggccgttgca gcaccgaccg aagaaaggat ttgtcccgac aaacgctcgt ctactcctcg
     7201 caccggcaca atcaccacgc gcgttgacac ttgccggaat tggaatagga tgagggggtc
     7261 ccctaccgga aggcgttcca tgccgggcaa cagccagagc acacagctcg cgattgaaaa
     7321 agccctctac cgcatcgggg aagtcagccg cctgacgggc acgaaggcct ttgtcctgcg
     7381 ttactgggag acggagttcc ccatgctcca gccggtgaag agcccgagcg gtcaccgggt
     7441 ctatcgcaag caggacgtcg agatggtgct gaaaatcaag cgcctgctct acgacgaggg
     7501 gttcaccatc gccggtgcgc ggcggcacct gcgcgagctc aacggcaatg ccgcaaggga
     7561 accggccggc tcagcggaag gcccggcaca actcttgagc cggaaaacgc tgctcgactt
     7621 gcgcgatgct ctgcgcggtt tcttgacgct tctcgaacgg cggtgatagc ctatgttcgt
     7681 cacgcgcggc cgccaagcgg tcggcgtttc ggtcgggacg tagcgcagcc tggtagcgcg
     7741 cacgcttggg gtgcgtgagg tcgctggttc gaatccagtc gtcccgacca tttgctttca
     7801 acgtttcctt caggcttcga ttttaccata agggacaagc gtgcgactgc gtgctatcac
     7861 gatgacttcg cttctcatct ttagtagaat ctgctcatga cgatccaaac aaatcccgag
     7921 atgttcagcc caaagcttcc ggttcatacg gtgacgcaca ttgaccgggc tgctggcgtt
     7981 gtcacgctga gcggatcgga cggctcgtcg cagcaggttc ggctggattc ttgggggtcg
     8041 tttgaagaag ctcagccctt gatcggcagc aaagtccagc tattgccttg agtccactta
     8101 cttgtcaccg cttcgccgtc agaacaaagg atgcacccaa aaatagtgaa caaccgtgac
     8161 gagtagccag attgctcctg ctattgaagc aaccatgaca gcgaatcgga gcacgcgccc
     8221 agtgctttca ttgcgtcgct gagtatcccg ccattctcgc tgttgctgtt cgtatgcagc
     8281 ctctcgctgg cgatcttctt ccacgatccc cggaatcgct tgccaagctg ccgtttccga
     8341 atcgcaagtg gcacgcactt tgtcttcctt ttctgtgaac gacaagccgt cgaaggattt
     8401 taccgtgtaa ggctcttgac gattggttcg gctttcttcg tttgtaatta cgcaccacag
     8461 ttccggcaag ctggaacgtc gagcagcttc atgctccgcg tcagggcgag acataccgct
     8521 gcacacggtc ttcgccccca gtgaccagaa gttacgaata tcgttttggc atactgcttc
     8581 gaagcgcgac gtttctggat gttcagcacg atagctggcg gaccaccaaa ccacttccca
     8641 agcgaacaag gtttccttcc cgccctcagc agcaatcgca gcagcgttgg ctgtggtctc
     8701 cgtgctgtac cggactctgc actgaatgtt gctgtactga ttgagatggc ttataggaat
     8761 tacggcatag cgtggcgtac ccggatgtag ctttcgaagg tagtccgagc actcgaccac
     8821 ccaccaccac tctttcggct gctcataatc ggcaaggttc acgggggtaa cttaattgcc
     8881 taccgcccaa aattcaagag gaacctaaaa cgggcttctc tctctaaaag tttgtataaa
     8941 cgaattcacc aaacgccgca ccacgattcc agagccttcg cgaccagcgg gcgctcttcg
     9001 cggacgcccg ctggatttgc aaattgtctc ggcgacggtc tctcagcgcc gccagtccct
     9061 aactgttacc accagcccta cagtctgtta gatgccagcg gcacagaaaa gtcgcacagc
     9121 aggctgcggt gcgccgcgac catgtcgccc gtctggagtg agacgtatcg ccgggacatc
     9181 tccaagtcgg aatgtcctaa ggccttttgt aaatggaaga ccgatccccc tcttctgaca
     9241 tagttcagcg cgaaggtgtg tctaaaggca tggagtgttc ggcgtggcgg cgcgaagccc
     9301 agtctgcggc agagcgtctt gaccctgcgc agggcgctgg aacggtcgaa gcgccacaga
     9361 aagggacaag ggtctgcgcc tctctgtcgc cacagcgcct tgcgaagctc atggctgaac
     9421 ggcacgagcc gatccttccg ccctttgccg tgtagggtga gcaggcagtt gtcccaatcc
     9481 acatccgacc attgaagtcc gagagcctcg ctgatcctgc atccgctgtc cagcagcacc
     9541 aacacgagca gatgcaatct gtcgtgcggt ttgcttgtca ccagcagctt gatctgaatg
     9601 tcagagaacg tcggaagtac aaattccggt tccttaagct gaggaacctt atgctgacta
     9661 ccagaccatt tcagatagac gttaaccgca cggatcgctg cgttcgcccc cgttggcttg
     9721 aggccagctt cgcgcatagc catgaccatt tgcttcaact gatcggcatc cggggattcg
     9781 cacggcagcc acttcgctgc gtggcgatac catgacaatg tggcgatgga aacattctga
     9841 agatactgct tctctttgac aaactgttcg aagcgtgggt acattgttcc tcctgtttct
     9901 tgaggaacgt tgaaaacacg gacgatgacc gagcggctgg aggcgcacgc ttggggtgcg
     9961 tgaggtcgct ggttcaaatc cagtcgtccc gaccattctt cttccggaaa cgcagcctgc
    10021 tcatctttcc cgcgccggac ggaatttgcg tattctaatt gtgtcgccgc gcaggcggca
    10081 ggacccgaat gccaatgact ttaaataagc cgcacatcct gctgacgaac gacgatggct
    10141 ttcacgcccc cggcctgcac gcactggccg cggcgctcga ggactgggcc acgctgagcg
    10201 tcgtggcccc gaagcgcgaa cagagcggcg cggcgcagtc gctcaccttg cgcactccga
    10261 tcgtttgcca tcccatggcc gagcggcagt gggccgtgga cggcacaccg gcggactgcg
    10321 tgattgttgc gctgcacaag cttcttccca agccgccgga cctggttctc tccggaatca
    10381 atctgggagg caacctgggc gaaaacgtct attactccgg cacggtggga gcggcgcgcg
    10441 aggccgctct gcaccacatt ccttcgtgcg cgatctcgct ctgcgcgaaa cacgcgccgg
    10501 cgcgcttcga cgatgccgcg cggatcgccc gcgcgatggc cgagttgatc ctacgcgagg
    10561 gcctgccgga tcaggtcctg ctcaacgtca atgtaccgga ctcctgggaa ggcggcgtga
    10621 aattcacgcg ccagtcgcgc aagatcaccc ggaatcagct ccaggaaggc gttgatccgc
    10681 gcggccgcgc ttactactgg ctctttgagc agagaatcac caaagatgtg gagccggata
    10741 cggattacgc cgccatcttt accaacgcgc tctccatcac cccgctgcac ctcgacccga
    10801 cgcacacgga gtctctcaat cacctctccc gctgggcaaa gccgctcgca gccgcattcc
    10861 agcgatgaat tggcgccgcc cggccgcctg cggtcaaggc atttgctgaa acccaaattt
    10921 gtttgttcgt cgaacttgct tccacgcagg cccaaaggca aatccattcg tctgacagtc
    10981 gggaggctgg tatcatggag gcattggaac caatggagtc caaggcgatg aatatcaatc
    11041 tgacgcctga acagcaaaag atcgtcaatg acgagctcaa ggccggccac ttccgcaccg
    11101 tcgcggaagt gattgccgag gctctgcaag ttctccgcga aaaagaacga tctgcggacg
    11161 ttggaagtcc caacggtgcg cagcgcgaag ccgtgcgcga aatgctcgcc ttcgttgaga
    11221 agaaccgcgt gcgcctcgaa ggggtctccg tcaaagaact gattcacgag ggccaccgtc
    11281 tgtgagtgat ttcgtgctgg acgcctctct ggcgctccag tggtttctgg aagacgaagc
    11341 ggatcgaaag tacagcctcg ccgtccttgc aagcctctcc gaaaaccgtg cggtggttcc
    11401 cctcctatgg ttctatgaag ttggcaatgg gcttctaatg gcctaccgcc gcaagcgaat
    11461 caccctcgat cagattgatg gctttctgac gcgattaaag gcccttccaa ttgacgccgc
    11521 gcagcagact ccctccgaaa tcctcgaatt gcccaccctt gcgcagacgt acagcctcac
    11581 gaattacgat gcggcctacc tcgcgctcgc catgcgctcc aattcgtcat tagccacgac
    11641 cgacattgac cttcgcaagg cagtcagcgc cgccggtctg aggattgtga cggcgtagct
    11701 gggcaaagcc gttctcagcc gcatttcgac gctaagtctg ctgcgctgtg ccggtcttca
    11761 ctgggcgatt ctgcctccgg cgagccggaa acggctccac gatccccatg gtttgacagc
    11821 ccccgttccg caagggagaa tcgctccggc gaattgttcg cgagccggag aaacaaggcc
    11881 acgagcaaat gaaattccat ctggatcgga gaagttttct caaggcggtg gccctgggta
    11941 ttgctcctct cgggcacggg aggttctcaa tggggcgact gctatcgaag ggcggcttgc
    12001 cgggcacgcg cgcgggaggc agggcagcga tgggtggcgt gaagacttac gatgccgcgg
    12061 tggtgggcgc cggcgtgttc ggctcgtgga cggcatggca cctggcgcag gcggggaaaa
    12121 aagtgctctt gttggacgcc tacggaccag cgaactcccg cgccagttcc gccggtgaat
    12181 cgcggatcat ccgctccggc tacggccccg atgcgcttta cactcgctgg gccgtgcgct
    12241 cgcttgttct ctggaaggag tttttcgcgc agacagggat cggtcttttc cagaattgcg
    12301 gtgtgctctg gctcgcgcgc gaaaaggact tctacccgaa acaaaccgcg gagacgctca
    12361 cgaagatggg tgtcccattc gaacgcctcg agcgcaccgc attgcagcgc cgattcccgc
    12421 agttcaattt ggacgacgtg gactggggcc tgttcgagcc cgaagccgga gcgctcctcg
    12481 cccgccgcgc ggtccaggcc gtggtggtgg ccgcagttcg aaagggcgtc acctacgagc
    12541 aggcggcagt gaatccgccg gcggccaagg ggagactgga gcgcattgaa acgagcagcg
    12601 gcgaacgcct ctcggccggc gagttcgtct tcgcctgcgg cccctggctc ggaaaagtgt
    12661 tcccgtccgt gctgggtgaa cggattttcc cgagccgtca ggaggtattt ttcttcggcg
    12721 tgcccgcggg caacgggcaa ttcgcgtctc cagcgatgcc ggcgtggatt tgcctagcgg
    12781 acgaagtgta cgggctcccc gacctcgaga gccgcggaat caagatcgcg ctggatcgtc
    12841 acggcgagcg cgtggacccg gacagccagg cgcgcatcgt ttcgaaggag accacggaat
    12901 ggatgcgcag ctacgtggac aggcgcttcc cggccctgcg cggcgcgccg gttgtcgaga
    12961 cgcgcgtctg ccagtatgaa aacacttcca acggtgattt cctgatcgac cgccacccgg
    13021 agatggaaaa cgtgtggctt gtcgggggcg gctccggcca cgggttcaag cacggccccg
    13081 cgctcggcga atatgtcgcc ggccggctgg cgggcggagc agcggaggag ccgcgtttct
    13141 ccctggcgac gaaacgcacc gtacagaaac gcgccgtcta ctgaactatg tcgagccgcc
    13201 cgcgccgcaa ggccgtcgaa tctttcgccc gctgctacgc cgtttcatcc gaggtgtggc
    13261 cgccgccgcg cccgccgcga tttatccaag tacagctttt catccgcctg cgagagcagt
    13321 ttctcgatgc gctcgccgtc gccgcaatag atggagatgc cgatactcgc ggaaatcggc
    13381 ggcttctcgt gatccctggc caggacttcc cggatgcggt tggcgacgcg cttcgcatcg
    13441 ctctccggcg tctccggaag cacgagggca aattcatcgc cgccgtagcg ggcggccgtg
    13501 tcgatggccc ggcagtgaac tcgcagtatc tccgccaggc ggcagagcgc gcgcgtgccc
    13561 accaaatgtc cgtaggcatc gttgattttc ttcagcccgt cgaggtccag gagtaaaacg
    13621 gcgaagggcc ggccggtgcg gttcgtccgc tcggtttcgc tttccaggac gtcgagcagg
    13681 cggcggtagt tggcgaggct cgtgaccgga tcgctggtgg ccatttgccg caccttttcg
    13741 aagagcctcg cgttatccag cagcgccccg ccgagagccg ctgcgaagcc ggtcacttgc
    13801 agcccttgtg ccagcgcgaa cggtgcgtcc aataagtgca ccgactggga cgcggcaagc
    13861 tgtgccgcaa gattgagcca ggccgccgcg taaaccatcc ggtcaaaggc cgagtccgac
    13921 ctcgagatgc ggcggcgata tccgatcagg gccgccagga aaaacaacgc gggaatgagt
    13981 tgcagggggc tgggaaacac ggcgcccggg tgcgccgagg tctccggcgg gtgagctcgg
    14041 agcccagcac tgatcaagta ggtgagggtc acgaccgcca gcagtgcgcc ggcgatctct
    14101 cggcgcgggt ggcgggatct cggcaggaac cggtccacaa gaatcgccac gacaaacagc
    14161 actccgagca gcatgcggct gacccaccag gcaaccggcg cccagaaaaa ccgcaggggc
    14221 acgtcacgaa ggagctggaa aaacaaaacg ctggacgccg cgagtaccgc tcccgagagc
    14281 aggaacccgc tgcccagaat gagcgaaatc cgatcctggg agccctgaaa tcgcacgaga
    14341 gcaacggcca cgaataccag cgccagcaaa cctgtgatga cctggagata tccgtgcagt
    14401 acggggtccg ttttgatcgg cacatgccga agccacatgg caagaccgag aactgcaaga
    14461 aaaccggcag agtggtaaag gaatatggat cgagaagacg cgcccgagtt tattcgcggt
    14521 ccaagcatgg cccgtgaggt aggggaggct gagaagatgc ttccaagata gcccaagggt
    14581 tttcccataa cagcactttc caagactagg atgcgcgaaa gtgcgccggt ttgaaactgg
    14641 acgagagaac aggtgcacca cacttgtcaa tccagccgaa cgccagccgc ccgcagctcc
    14701 gactgcagct acacacccgt tcccgtgtga tcggtccgct gcccggcgga gcgcttgcag
    14761 acgcgctgct gcgttccctc tcccttttgt agcgctcaac tgggcgtagc gaaaagaatc
    14821 tggactacaa ttgggcctgt cttgaatgag gcagcggccg caccccgcgc gccaaggggc
    14881 gattctcacc atgagcctga tgcgttccat gctgctcgca gcttcccaga gccgctggct
    14941 gcgcgagcac gccacgcggt acagatttat ccgccgcagc gtcaaccgtt tcatgccggg
    15001 cgagtcgctg gacgatgctc tcgcggcggc gcaggcgctg cagagcaaga aactcgatgc
    15061 ggtgctcacg cagctcgggg aaaacgtgaa ggaggcatcg gaggcgcaga aggtcgcggc
    15121 gcactacgcc gaagttcttg accgcgtcca cgcgacgggt ctccgcgcag agatttccgt
    15181 caagctcacg cagctcggcc tggacctctc tcccgatctc tgcttcgagc acttgaaggg
    15241 catcgtcgcg cgcgaacgga aggaagccac cgtctggatc gacatggaaa cgagcccgta
    15301 tgtggacgca acgttgaacc tctatcggcg cgctttgcat tcctacccga acataggtgt
    15361 atgcctgcag gcttaccttt accggacgaa ggaagatctt gccagcctgc tgccgctacg
    15421 gccctcgatt cggctggtga aaggagcgta caacgagccg ccgcacgtcg cgtttccgaa
    15481 gaagcgcgac atggatgaaa actatttcgc acttgcgcag gaattgctcc gggcaaaaaa
    15541 agagaatcgc tgcgcgagag cggctttcgc cacgcacgac gtcgcgctca tccggcgcat
    15601 cttcgatgcc gcatcgcggg agggtttctc cgaaagagat ctcgaagtgc aaatgcttta
    15661 cggcatccag cgcgccgaac aggagcggct cgccggcgaa ggctgccgca ccggcgtcct
    15721 cgtctcttat ggaagttact ggtatccgtg gttcgtgcgg cggctcgcgg agcgccccgc
    15781 gaatctctgg ttccttgcgc gcaatatttt cgcaggctga tctattccgt tgccgatccg
    15841 ctggttgggt acgcatccaa ccgcttcttg tagagcagga gcacgctcgg accgaaagac
    15901 aacaacatga cggcgatccc agcggctatc ccgaaactgg tcgcggccgg cgccgaacaa
    15961 tcgtgacggc gccgcacaat gacatggcat gggacggcca ccaggagttc caagacgctg
    16021 cctttcagca accaggaaac ggctcgtgtg agcaactcgg tggagtttcg gaaatacagg
    16081 taaaagagaa tcccccagaa caaccacagg aaagcccaca tggccaggac cttcgcctca
    16141 ctattgaaaa aaggggagtt gtcgagcttg tcgccatgga tggcagccgc aagagaaaac
    16201 agggccgcga aggtaagaag agccagcaac atcgctgtga gggaactgga aactagaatg
    16261 tgcgcccgcg gcttgagtcg ttttcggctc gcatcaacgg acaagaaaag cagaagcgcc
    16321 tggccggcca ggacgatccc aatgggaatc caggacaacc actccaagta gacgcccctt
    16381 acacccccga aaaactcccg ccagctcgag gacttatctc cgtagagaaa taccgccccc
    16441 ggcaccagga gacccagaac aatcacggcg taaaagactg ttatgagtat gccccacttt
    16501 cgcatttctc ctccttcggg cggttaaggg atttaccacc cgccttggaa gaacgcggag
    16561 gcaaagttcc agattggtat cctcttatcg tgggttaccg tcaagaagaa aaaaggaatt
    16621 caaaaagggt gcaccgttcg ctttgctccc atcgaggagg cttccctcgc ctgcctctgc
    16681 cgggctcggg gcaagcaggg taaacggcgc ttcgcgagac gaaaccactg cggcacaagg
    16741 atgaggagtc agaccgttcc ccgaccagac cgagtttttg tcccagccag acgggtcgcg
    16801 aatccagacg aagcccggac ggacctgagt tacgcggcct gcgcagcaga agcgagctcg
    16861 gtgaggacac actccgccct ctgcttgagg agctgagcct gcgcatcatc cggatgcaag
    16921 gtgagacgat cctcgaggag gctcaagaga ttgcgcacca tctgaaccgt ggccggccct
    16981 cggcccgggc gggtggcgtc cgtgacgaag ctggcacgtt gcgctggggt ggcgtgaagg
    17041 agtacgtagc gccagtttct gatctgggag atgcaaataa tcttttcgtc acgcagaccc
    17101 tgtatctcct ccacgtggaa gcgcggccag aggagacgcc cggtcaggaa atccataatc
    17161 ggcaagacga tgtcgtggtt atggtcttca tcgtagtggt gcaagatgtg gagcttgaac
    17221 agccactgga agtacctgct cctcgcccag cgcggatggc tggtggcgac atcctcatgg
    17281 aagcggtgat gcacgacgga aacaacccac tgggcataga cgtacacggt ggcgacaaag
    17341 agggcggtgc cccagttgag gccgttgatc gcgatgaaag ccgcgcccac gatgaacccc
    17401 gggatgtacc attcccagga gaagccctga accgctccca catacttgac gtttcggaca
    17461 aagagctcgc cgaacggata ctgccaccag tggtgaatct tgtggtctcg ctgggcgtcg
    17521 cggacgaatt cgccgagggc tctcgccagg atctttccga aaaatggccg gacatggtcg
    17581 tcaatccgcg tcatccagcc aacgtgttcc acaaagcggt ggttcacgta gccgatcacg
    17641 gtcaccacga tgatcgctcg aaggacgatc agaggcaccg taacccacca ggtcggatag
    17701 ccgcgagcgg caagcaggcc gccgaagaag tggttgctga acggcaacag ggcaaatgag
    17761 atcagcgaaa agacaagaaa gatcgtccag gcgctcgaca ggattcgact cagcaaactt
    17821 ggttgtttga gcagagaacg ctcgtaaatc tgattgttca gctcaaccgt cgaaccggcg
    17881 ttcatagcgg tactgctccc tcgcatggac cccaaacgac aggcatccag gaaaccttat
    17941 tgataataaa ccaggggcaa atgcgatgtc cgcggccgga ccggccgcga gtgactccgc
    18001 gcccaaacag cggatcgttc aggttgcaag ataccctttc ttcgaggtca tcgtaaagat
    18061 gaaattcgca ggctgtcctc aattggcaga atggcggtga cggtcccaca agacccgttc
    18121 gctgcgaacc aggaggtttc gtttaactcg atgccgcgcg cacgccgggc tcagttgcca
    18181 gcgttgcggg ccaattttgc cgtgttataa ttgcgcgcat gaaaggaatc cttgttcgtt
    18241 cgctggcggt ccttctatcc cttatcctgc tgcccgctcc ggactgggcc acgtgcggag
    18301 gagggggtgg cggcggtatg ggcggcatgg gtggcggcgg cacgtccgac caaaaggtct
    18361 acaccgtgcc gtggaagctc atcaagccgg gagacccgcc g
//
LOCUS       DASYVO010000167         6462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_132383, whole genome shotgun sequence.
ACCESSION   DASYVO010000167 DASYVO010000000
VERSION     DASYVO010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132383"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1798)
                     /locus_tag="VGR03_05675"
     CDS             complement(<1..1798)
                     /locus_tag="VGR03_05675"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEV1993799.1"
                     /translation="MKYDDFDLQIEPAGEKFRVRLLNAPTGQATTEFVLPFTEIEVGN
                     FLSRIGQVRRTMRRLDAPELQAAKEFGGKLFGAIFSGEMIAQLRGSMEQASDKEHGLR
                     IRLCLTDVPALADLPWEFLYDANQNHFLTTSSETPVVRFLDLPQRIAPLRVALPLRVL
                     VMIASPRNLKRLDTEGEWGRLQESLADLLRGGQIRLERLPAATLDALRLRARGAPFHV
                     FHFIGHGGFDEAAQDGVLQFEDETGMSYPVRGEMLGMQLHDHRSLRLAVLNACEGARS
                     SRQDPFSGVAQSLLQQRVPAVIAMQFEISDAAAKVFASEFYRAVAEGNPVDAAVCESR
                     KALFKEEFGQEWATPVLYMRSQEGQLFELQAVAAPPLPDEDLLKRELEEAQNQAAAKA
                     EAERAAKVEKERLEREKAEKDRLAFAKAEADRQAVAKAEAVRVAAQRAEAARAAQAEK
                     ERRGREKAEQERLANEKAAAERRAVEIPRELAEEAMREGNLIREKLRAGIRAAEQARA
                     EAADQAEKERLVLEKTARENQAREKAESERQAAARAEAERAANAGKERQAKEAADRAR
                     SLRQQEEAERRAKERAEYERLQREKTEADRRAR"
     gene            complement(1808..2146)
                     /locus_tag="VGR03_05680"
     CDS             complement(1808..2146)
                     /locus_tag="VGR03_05680"
                     /inference="COORDINATES: protein motif:HMM:NF041216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CU044_2847 family protein"
                     /protein_id="HEV1993800.1"
                     /translation="MSQFAQFPLKDGGFLVVEVAGAESPGSRVMRGSAAEDAIAKATD
                     TFESALENVRSAAEGILHQLRSLAQPPDEVAVEFGVKMSAESGAIIAKASGEANFKIN
                     LTWKKSPAGK"
     gene            2499..3470
                     /locus_tag="VGR03_05685"
     CDS             2499..3470
                     /locus_tag="VGR03_05685"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEV1993801.1"
                     /translation="MATTGHIAASPRESTAQPGRTGESLLDRIGNTPLLRLEHVGQEF
                     PNIEFCAKAEWFNPGGSVKDRPALNMIQAGLASGTLRPGKTIIDATSGNTGIAYAMVA
                     AALGFPVQLCLPDSASHERKRILSALGAELVITPGDEGTDGAIRKVHEIVANNPEKYF
                     YPDQYSNPANWQAHYRTTANEIWQQTSGRISHFVAALGTSGTFVGTTRRLKELNPAIR
                     CVSLQPDASFHGLEGWKHMPTAIRPAIYDDSLADENLAVSTEEAYRMVKRLAREEGLL
                     VSPSAAAALLGCFQVAAGIPKNANAVIVTVFADSASKYLNERFWDEE"
     gene            3473..3961
                     /locus_tag="VGR03_05690"
     CDS             3473..3961
                     /locus_tag="VGR03_05690"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEV1993802.1"
                     /translation="MADAAALWIGGHLAEKIRAHGAETYPHECCGALLGRDSSAVLES
                     DRGKGALTPAREILRLFPLVNRRDDSPRNRFSVTAEDVRAAEKAAREQGLEVVGWYHS
                     HPDHPARPSEYDRDHAWPWYSYIIVSVQNGAPQDMTSWRLNDDRAEFSPEGIEIRHRA
                     AV"
     gene            3993..4274
                     /locus_tag="VGR03_05695"
     CDS             3993..4274
                     /locus_tag="VGR03_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEV1993803.1"
                     /translation="MPVKVMIPSPLRPYAGKRDSAEFNARTVGEALGHLTAEFGELRK
                     HLFTEDGKLRSFVNVYVNDEDIRYLAKENTPTKDGDTISIIPSIAGGRI"
     gene            4383..5567
                     /gene="moeB"
                     /locus_tag="VGR03_05700"
     CDS             4383..5567
                     /gene="moeB"
                     /locus_tag="VGR03_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEV1993804.1"
                     /translation="MATTAQPQTGTKPAASLSNQEILRYSRHLIMPEVGMEGQLKLKN
                     AKVLLIGTGGLGAPLGLYLAAAGVGRLGLVDFDVVDFTNLQRQVTFGTSDVGKPKSEA
                     ARARLANLNPDIQIQSFETQLTSANALELFKDFDVIVDGTDNFPTRYLVNDACILLGK
                     PNVYGSIFRFEGQSTVFGMPDGPCYRCLYPEPPPPGLVPSCAEGGVLGVLPGIVGSIQ
                     AMETIKLILGRGDSLVGRLLLFDALGMKFRELKLRKNPNCPVCGTHPTITKLIDYYEF
                     CGVRGEEAPGPSVQVPEITPRELKARLDRGDDLFILDVREPHEYQICNLEGHLLPLGE
                     LTRRVHELDSSREIVAHCRSGKRSAEAVDFLRKAGFRKVLNLKGGILAWSDEVDPSVP
                     KY"
     gene            5687..5920
                     /locus_tag="VGR03_05705"
     CDS             5687..5920
                     /locus_tag="VGR03_05705"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEV1993805.1"
                     /translation="MAATKITVNHNGSIRVEGEFEIFDPEGKPFGLAGRTTIGLCRCG
                     HSANKPFCDGSHKTCGFTDTVGARELPPPKPKL"
     gene            6231..>6462
                     /locus_tag="VGR03_05710"
     CDS             6231..>6462
                     /locus_tag="VGR03_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993806.1"
                     /translation="MGSWGKIIGFLRSDRDLVSAQNVHRRVLVVSGAMSLVLFLAVVF
                     HLRQVSVAGSPQGYLYLEVGGTLLSFCYAANAL"
BASE COUNT         1258 a         1967 c         1840 g         1397 t
ORIGIN      
        1 cgcgtgcccg gcgatccgct tccgtttttt cgcgctggag ccgctcgtat tccgcccgtt
       61 ctttggctcg gcgctcggct tcttcctgtt gacgaagcga tcgcgcgcgg tctgctgctt
      121 ccttcgcctg gcgttccttc ccggcatttg cagcgcgctc cgcttccgct ctggcggcgg
      181 cctggcgttc ggactcagcc ttttcccgtg cttgattctc tcgtgcggtt ttctcaagga
      241 ccaatcgctc cttctctgct tgatcagccg cttccgccct ggcctgctct gccgcgcgaa
      301 tcccggcccg aagcttctcc cgaatcaagt tgccttccct catggcctct tcagcaagct
      361 ctctcggtat ttctacagcc ctgcgttcag ccgccgcctt ttcgttggct aggcgctcct
      421 gttcggcttt ttcccgtccg cggcgttctt tttctgcctg cgcggcacgc gctgcctctg
      481 ctcgctgtgc agccacgcgc acggcctctg ccttggcgac cgcctgccgg tcagcttccg
      541 cttttgcaaa ggctaggcga tccttctccg ccttttcacg ctcaagacgt tctttttcca
      601 cttttgctgc gcgctcggct tccgctttgg ccgcagcctg attctgagct tcctcgagtt
      661 cccgcttcag tagatcttcg tcggggagcg gcggcgccgc aacagcctgc aactcaaaca
      721 actggccttc ctgcgagcgc atgtacagga cgggcgtggc ccattcctgc ccaaactctt
      781 ccttgaagag cgcctttctc gattcgcaaa cggcggcatc caccgggttg ccttccgcca
      841 cggcgcggta aaactccgaa gcaaacactt ttgccgcggc atcgctaatc tcgaactgca
      901 tggcaatcac cgcgggcaca cgctgctgca agagactctg ggccacgcca gaaaaaggat
      961 cttgacgcga acttcgggcg ccctcgcacg cgttcaacac tgccaacctc aacgagcggt
     1021 gatcgtgcag ctgcattccc agcatttcgc cgcgaaccgg atacgacatt cccgtttcgt
     1081 cctcgaactg tagaacgcca tcctgcgcgg cttcgtcaaa acctccgtgg ccgatgaagt
     1141 ggaagacatg aaacggtgcg ccgcgcgcgc ggagccgcaa ggcgtcaagc gtggccgcag
     1201 gcaaacgctc cagcctaatc tgcccgccac gcaataaatc cgcgagcgac tcctggagcc
     1261 ggccccattc cccctcggtg tccagacgtt tgagattcct tggactggcg atcatgacca
     1321 gcacacgcaa tggcagcgcc acgcggagcg gtgcaattcg ctgcgggaga tccagaaagc
     1381 gcacgaccgg ggtttcgctc gaggtggtca ggaaatgatt ctggttggcg tcgtacaaga
     1441 attcccacgg caggtcggcc agcgcaggaa catccgtgag gcacagccgg attcgcaatc
     1501 cgtgctcttt gtcggaagct tgttccatgc tgccccgaag ctgggcgatc atctctccag
     1561 aaaagatggc gccgaaaagc ttcccgccaa attccttggc ggcctgcaac tcaggggcgt
     1621 ccaggcggcg catggtgcgg cgaacttgcc cgatgcggct cagaaaattg ccgacttcaa
     1681 tctcggtgaa gggaagtacg aactccgtag tcgcctggcc ggttggagcg tttagcaaac
     1741 gaacccgaaa cttttcgccg gccggttcaa tctgcagatc gaaatcgtcg tacttcatag
     1801 tcacggctta ctttcccgca ggacttttct tccacgtgag gttgatcttg aagttggctt
     1861 cgccggaagc cttggcaatg atcgcgccgg attccgcgct catctttacg ccgaattcga
     1921 cggcaacctc atcgggcggc tgtgccagag agcgaagctg atggaggatg ccttccgcgg
     1981 cgctccgtac gttttccagt gcggactcaa acgtatcggt ggctttagcg atcgcatctt
     2041 cggccgccga accacgcatg actcgtgacc cggggctctc cgccccggct acttccacga
     2101 caaggaagcc gccatctttg agcgggaatt gcgcaaactg ggacatcggc acctctaacg
     2161 gttccctccg cgccgaccct gctgggtaaa ctcgtttgca ttctgcataa ttactgcgag
     2221 catacccctt cactcccttt ggagcaatga cgtgtgcttt atcttttggg gagccccaac
     2281 cggtccagtt cacgaggccg aaactgctca atcgcccgca acccggcttg ctccgccatt
     2341 ctgcttataa ggattgtgag tcctatggtt cctgcgtttc acagtttcgt gaaatggagg
     2401 cctatatctc cgggctaggc tggcgcatct aattcggagt aaaatggtcg gatatcccgt
     2461 gacctcttaa ccgggaggag aggctcaaaa ccaggcgtat ggctaccacg ggccacatcg
     2521 ctgcatctcc ccgcgaatca acggctcagc cgggccggac gggtgaatcc ttgctggacc
     2581 ggattgggaa cacgcccttg ctgcggctgg aacacgttgg ccaggaattc ccaaacatcg
     2641 aattctgtgc caaggccgag tggttcaacc ccggtggatc cgtaaaggac cggccagcgc
     2701 tgaacatgat tcaggccggt ctcgcgagcg ggacgttacg gcccggcaag accatcatcg
     2761 atgcgaccag cggcaatacc ggcattgcct atgccatggt tgccgcggcg ctgggttttc
     2821 ccgtgcagct ttgcctgccg gacagcgctt cgcacgagcg caagcgcatc ctctcggcgc
     2881 tcggcgcgga gctggtgatc actcccggcg atgaaggcac cgacggtgcg attcgcaaag
     2941 tacacgaaat cgtcgcgaat aatcccgaga aatattttta cccggaccaa tacagcaatc
     3001 ccgcgaactg gcaggcgcac tatcgcacta cggcaaatga aatttggcag cagacctccg
     3061 gccgcatctc gcattttgtt gcggcactcg gtaccagcgg cacattcgtg ggaacgacgc
     3121 ggcggctcaa agaacttaat cctgcaatcc gctgcgtgag tttgcagccg gacgcatctt
     3181 tccatggcct ggaagggtgg aagcacatgc cgacggccat ccgtccggcg atttatgatg
     3241 actcgcttgc cgacgaaaat ttggcggtct ccacggaaga ggcttaccgg atggtgaagc
     3301 gcctggcgcg cgaagagggc ttgctcgtca gccccagcgc agcggcggcg ctgctgggtt
     3361 gcttccaggt tgcggctgga atcccgaaga atgcgaacgc cgtgatcgtt accgttttcg
     3421 cggattccgc ctcgaagtac ctgaacgaac gtttctggga cgaagaatag gcatggcgga
     3481 cgcggcagca ctctggatcg gcgggcatct cgcggagaag atccgcgcgc atggtgcaga
     3541 aacttatccg catgaatgtt gtggcgcgct actgggacgc gattcgagcg ctgtccttga
     3601 gagcgatcgg ggaaagggcg cgctcacgcc cgcacgggag atactgcggc tttttcctct
     3661 cgtcaatcgg cgagacgatt cgccgcgcaa tcgtttttcc gtcaccgccg aggacgttcg
     3721 cgcggcggag aaggcggcac gcgagcaggg ccttgaagtg gtcggctggt atcattcgca
     3781 tcccgaccat ccggcacggc cgagcgagta tgaccgcgat cacgcctggc cgtggtacag
     3841 ctacatcatc gtcagcgtgc agaacggcgc gccccaggac atgacctcct ggcgtttgaa
     3901 cgacgaccgc gcggagtttt cgcccgaggg gattgagatt cgccaccgcg cagcggtgta
     3961 gcgcagcggc ttcagcacaa cggagaaatg aaatgcccgt gaaagtaatg attccatcgc
     4021 cgcttcgccc ctacgcgggg aagcgtgact cggccgaatt caacgcccgg actgtcgggg
     4081 aagcgctcgg ccatctgact gcggaattcg gggaattgcg caaacatctc tttaccgaag
     4141 acggcaagct gcgcagcttc gtcaacgtgt acgtgaacga cgaggacatt cgttatctcg
     4201 cgaaagaaaa cacgcccacg aaggatggag acaccatcag cattattccg tccatcgcgg
     4261 gcggccgcat ttagcagcat cgtttttgac tttggcaggg ctgggaaaga gttcgcgagg
     4321 aatctctctt tgttttacgt tcttacaaga tgaattgaaa tttgaaggag tagagaaacg
     4381 caatggccac aaccgcacag ccgcagaccg gaacaaaacc ggcagcgagc ctttcgaacc
     4441 aagaaatact acgctacagc cgccatctca tcatgccgga agtcggcatg gaggggcagc
     4501 tcaagctgaa gaacgccaag gtgctgctca tcggcacggg cgggctcggc gcaccgcttg
     4561 gtttgtatct tgcggccgcg ggcgttggca gactcgggct ggttgacttt gacgtcgtcg
     4621 acttcaccaa tctccagcgc caggttacgt ttggcaccag cgacgtcggc aagcccaaga
     4681 gcgaagccgc gcgcgcgcgc ctcgcaaacc tcaatccgga cattcagatc cagtcgttcg
     4741 aaacgcagct gaccagcgcc aacgccctcg aactcttcaa ggattttgat gtcatcgtcg
     4801 acggcaccga caatttccct acgcggtatc tcgtcaacga tgcgtgcatt ttgctgggaa
     4861 agccaaacgt gtatggctcg attttccggt ttgaagggca gtccaccgtt ttcggcatgc
     4921 ccgatggccc gtgctaccgc tgcctctatc ctgaaccgcc tccgccgggg ctggtgcctt
     4981 cctgcgcgga aggcggcgtc ctcggcgtcc tgcccgggat agtcggctcc atccaggcga
     5041 tggaaacgat caagctgatc ctcggccgag gggatagtct cgtgggacgc ttgctgctgt
     5101 ttgacgctct gggcatgaag ttccgcgagc tcaagctccg caagaatccg aattgcccgg
     5161 tgtgcggcac gcatcctaca atcaccaagc taatcgacta ctacgaattc tgcggcgtgc
     5221 gcggcgaaga agctcccggc ccgtccgtgc aagtgccgga gatcactccc cgtgaactaa
     5281 aagccaggct cgatcgtgga gatgatctgt tcattctcga tgtgcgcgag ccccacgaat
     5341 accagatctg caatctcgag gggcatctgc ttccgctcgg ggaactgacc aggcgtgtcc
     5401 acgagctcga ttcatcgcgg gaaatcgtgg cgcattgccg cagcggcaag cgttccgccg
     5461 aggccgtgga ttttttacgc aaggcgggct tccgcaaagt cctcaacctg aaaggcggca
     5521 tccttgcgtg gtccgacgag gttgatccgt ccgttccgaa gtactgattt gtcttcatcg
     5581 acttttgttg cgtgacaaga ccgccccggc ctgatcgggg cgtttgtcat ttgtgcttgt
     5641 tgctgcgcgg gagtgtgggc atactctcac tagctcagga gaaaaaatgg cggccacgaa
     5701 aatcactgtc aatcacaacg gttctatccg cgtggaaggc gagttcgaga tctttgatcc
     5761 tgaaggaaaa ccgtttggtt tggccggacg gacgaccatc gggctctgcc gctgcgggca
     5821 ctccgcaaac aagccgttct gcgatggctc ccacaagacc tgcggattca ccgatacagt
     5881 gggtgcccgg gagctgcctc ctccaaagcc gaagctatag gggctgctga gacggctgcc
     5941 aactgtttca aatgttgaga ctgtctcaga ctgagacagt ctcgaatttc tatctgatac
     6001 tggccattcg cccggtaact ctaacgaaac aaaaatgata gtgtcttaat ccgtgaaatt
     6061 gtaggtagtc gcggcgtctc gcagcgttcc aacgatttcc gtcctctccg atacactcca
     6121 ataagctatt taatttcaat gaattacggg aatgaatttc cggccagaaa agggcacaaa
     6181 cttgcattca tagggaattg tcagtttttt gtcggcattt gggggtcttt gtgggtagtt
     6241 ggggcaagat catcgggttt ctccgcagcg accgcgattt ggtctccgcg cagaatgtcc
     6301 acaggcgtgt cctggtcgta tccggcgcaa tgtccctggt cctctttctt gccgtagtct
     6361 tccacctgcg ccaggtcagc gtcgcaggct ccccgcaagg ctatttgtat ctagaagttg
     6421 gtggcacgct cctcagtttc tgctatgccg ccaatgccct gg
//
LOCUS       DASYVO010000168         6634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_132451, whole genome shotgun sequence.
ACCESSION   DASYVO010000168 DASYVO010000000
VERSION     DASYVO010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6634
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132451"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1420)
                     /locus_tag="VGR03_05715"
     CDS             complement(122..1420)
                     /locus_tag="VGR03_05715"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV1993807.1"
                     /translation="MSIEIEVGFENATKVQGVTEELLLEETPKGLANPKVKRLLIAGG
                     AVVLAAIVGLFVYYHNRETTDDAQVDGHITPMASKVYGRVAQVLVEDNQPVKAGQVLV
                     EIDARDYQAALDQAKASLALAESEARSAGVDVPRTRENVASGNSSADAQFMGAQADLA
                     RAQATYEQAQTADLAWAQANVDKSRANAELAKADLARYLPLMEKGEISKQQYDAAKAN
                     ADASASALQADLQKQAQAQRSVEVAKAQLDASKARVEQARAGVASAHADGKQVNMKAA
                     DAQAKLAKVEQARAALEAAELNLSYTEITAPVDGVATHKQVEPGQIVQAGQGLLVVVP
                     LQNVWVTANFKETQLKNMKAGQKVEVTVDTYGKTFSGRVDSIAGATGSVLSLLPPENA
                     TGNYVKVVQRIPVKIVLDPISSEKAILRPGMNVDATVITN"
     gene            complement(1432..3108)
                     /locus_tag="VGR03_05720"
     CDS             complement(1432..3108)
                     /locus_tag="VGR03_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV1993808.1"
                     /translation="MLRITTEKKRGKMFLSVEGRLAGPWVAALEQCWRELHAASPREK
                     FHVNLCGVSFIDAAGKVLLKEIHRQGGQLIAEGCLNQAIVHEIVARERQDTCNSEKER
                     RKSSHIIFYTVFFSLMLGPTGAHAQGTNKQSALPGNAPSQVLRLTLDQAVGLALKQNP
                     TAQIAILQAAQSEQDKNIARADLLPQANAKISDEAQKVNLQAQFGGKTPFPGFPKTLG
                     PYQVFSAGPSFGTPVFDLTLWRRYQAAQSTVNSSKANSLSTREQVILLVVSQYIGTLR
                     AMANVQASQSRVSLAQALYDQAADLQKEGVGTGIDTLRANVELQNEKQRLIEAETDRE
                     TSLFALSRLLNLDPRQSIELGDSLSFFDTPQPEVEPSMELALAERQEWKALESQIKAA
                     GYQKKASQDSRLPSLRFDGNFAYVGTSSNTTLPTYTYQASANMPLFTGGRIRAEIVRA
                     DLEIKKLDEQKDDLRNQIGLDMKTALLNLQSARNEVQVANLGVQLSKEEVDQARDRFK
                     AGVANNIEVIQAQDSLSRANDNQIVALYRFNQARADLARSMGQMEKVYAK"
     gene            complement(3332..4381)
                     /locus_tag="VGR03_05725"
     CDS             complement(3332..4381)
                     /locus_tag="VGR03_05725"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEV1993809.1"
                     /translation="MKRSNTVLLVAGVLFVLSMANLLRSRGHEVSASGPKASSVGSTL
                     IAAPGRVEAVSEEIRVSSELSGRLKAVHVEEGDRVQRGQVLAEIENDDYRARVAAAEA
                     ELAQREAELRRTVNGARTQERREAHASMQAAAAVLDNSKSEAERRRGLADRNVISRDE
                     AERYERGYQVARAQYEQAAQHFALLDAEAREEDRAHAEAAVATARAKLAEARAFLEKS
                     YIRAPINGVILRKLRHNGESVSTQFDSPVITMADDSVLRVRLDIDESDVSKLRVGQRA
                     YVTAEAYGTHKFWGRVIRVGRILGKKNIRTDEPSEHVDTKILETLVELDAGQPLPLGL
                     RVDSYMQSEEGPSVK"
     gene            complement(4400..5179)
                     /locus_tag="VGR03_05730"
     CDS             complement(4400..5179)
                     /locus_tag="VGR03_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1993810.1"
                     /translation="MKLSQAQTVIRAKGLGKIYREGATELRALQGVDLEVRAGELTLL
                     MGPSGSGKTTLLSILGCILRASEGKLEVLGKDVSALPESELPRIRREGIGFVFQGFNL
                     FPALTAAENVALALDVRGVRAGEAKKRGEELLAEVGLAQKARSFPADLSGGQKQRVAI
                     ARALAGDPPILLADEPTAALDWTSGRTVIELLQRLARVHGRAVVMVTHDPRVLSFGDR
                     IIHLEDGRIAREEQSVTSAMETSSFLEVHAAVPLISHQLSI"
     gene            complement(5255..6385)
                     /locus_tag="VGR03_05735"
     CDS             complement(5255..6385)
                     /locus_tag="VGR03_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1993811.1"
                     /translation="MGLLARRNLFHDKVRFAVTLTGIVFALVLIIIQFGLFLGFTTTT
                     SNNIDRSNADLWVVFHGVGYFDTGRNFSERKFYQVLSTPGVALAEKYMQAFGRWKRTD
                     GRVENIQVIGFHPGSGLGEPWNVVQGSVADLKQEDSVIVDELYRDKLGVSKIGDRVEI
                     GYHRARVVGFTRGIRSFTTSPFVYATFKNSLDYTNPSSSEFSTAYILVKAAAGVSPRE
                     LQERLRARLADVDVYTTAEFSRKTRFYWMFTTGAGLAVLTAALMGLIVGVAVVAQTIY
                     AATMDHIREYGTLKAMGATNRYLYRVLIEQAVWSAVLGYVAAMIVAYFIVRASEKGGA
                     VILMPWSMSIGMLFLAVLMCVAAALVSINKVTRIDPAMVFRG"
BASE COUNT         1175 a         2316 c         1598 g         1545 t
ORIGIN      
        1 aggggttgat tgtcccgtcc ggcatcctca gcttggctag aattctgtct cgcatcgcgt
       61 ctcatcactg gccctgctgt ttctacgtat tgcggttgca atccctatcc ttaattcccc
      121 gttaattcgt gatcaccgtc gcgtccacat tcatccccgg ccgcagaatc gccttctctg
      181 aagaaatcgg gtccagcacg atcttcacgg gtatgcgctg caccaccttc acgtaatttc
      241 ctgtcgcgtt ttccggcggc aacaggctca ggacggatcc tgtcgccccc gcaatcgaat
      301 ccactcgtcc gctgaaggtc tttccgtagg tatccaccgt cacttccact ttctgcccgg
      361 ccttcatgtt cttcagttgc gtctctttga aattcgccgt cacccaaacg ttctgcagcg
      421 gcacgactac cagcaaccct tgtcctgcct gcacgatctg tcccggctcc acttgtttgt
      481 gcgtcgccac gccatcgacc ggcgccgtga tctccgtgta gctcaagttc agctccgccg
      541 cttctagcgc cgctcgcgcc tgttccacct tggcgagctt cgcctgcgca tctgcggcct
      601 tcatgttcac ctgcttgcca tcagcgtgcg ccgaggcgac tcctgctcgc gcctgctcga
      661 ctcgcgcctt cgacgcgtcc agttgcgcct tggccacctc cacgctccgc tgcgcctgcg
      721 cctgcttttg caggtccgct tgcagcgcgc tcgcgctcgc atccgcattg gccttcgccg
      781 cgtcgtactg ctgcttcgaa atctcaccct tctccatcag tggcaagtaa cgcgccaggt
      841 ccgccttcgc cagctcggca ttcgcccggc tcttgtctac gttggcctgc gcccaagcca
      901 aatccgccgt ctgtgcctgc tcgtaagtgg cctgcgcccg cgccaggtcc gcctgcgcgc
      961 ccatgaattg cgcatcggcg ctcgaatttc cgctcgccac gttttcccgc gtgcgcggca
     1021 cgtccacgcc cgcggaacgc gcttcgctct ccgccagcgc cagcgacgcc ttcgcttgat
     1081 ccaatgccgc ctggtaatcg cgcgcatcga tctccaccag cacttgcccc gccttcaccg
     1141 gctggttgtc ttccaccagc acttgcgcca cgcgcccgta aactttcgat gccatcggcg
     1201 tgatatgccc gtccacctgc gcgtcgtccg tcgtctcgcg gttgtggtaa tagacgaaga
     1261 gccccacgat tgccgccagc accaccgccc cgccggcaat aagcagccgc ttcaccttcg
     1321 ggttcgctaa ccctttgggc gtctcctcca gcaacagttc ttccgtaacc ccctgcacct
     1381 ttgtcgcatt ttcaaatcca acttcgatct cgatactcat ggtctccccg ctcactttgc
     1441 gtaaaccttt tccatttgcc ccatcgaccg tgccaggtcc gcgcgcgcct ggttgaagcg
     1501 gtatagcgcc acaatctggt tgtcgttcgc gcgtgacagc gaatcctgcg cctgaatcac
     1561 ttcaatgttg ttcgccactc ccgctttgaa gcggtcccgc gcctggtcca cttcttcctt
     1621 cgatagctgc acgcccaggt tcgccacttg tacttcgttc cgcgccgatt gcagattcag
     1681 cagcgccgtc ttcatatcca ggccgatctg attccgcaga tcgtctttct gttcatccag
     1741 cttcttgatc tccaggtccg cgcggacaat ctctgcgcgt atccgcccac cggtaaacag
     1801 cggcatattc gcgctggctt gataggtgta ggtgggcagc gtcgtgttcg acgacgttcc
     1861 cacgtacgcg aaattcccgt cgaacctcag ggacggcagc cgcgaatcct gcgatgcttt
     1921 cttctgatat cccgcggctt tgatttgcga ttcgagcgcc ttccactcct ggcgctccgc
     1981 cagcgcgagt tccatgcttg gctcgacttc cggttgcggt gtgtcaaaga aactcagcga
     2041 atctcccagc tcgatgctct gccgtggatc caggttcaat aaacggctaa gtgcgaagag
     2101 cgacgtctcg cggtcagttt ccgcctcgat caaccgctgt ttttcgttct gcaattccac
     2161 gttggcgcgc agcgtgtcga ttcccgtacc cacgccttcc ttctgcaaat ccgccgcctg
     2221 gtcatacagc gcttgcgcca gactcactcg cgattgcgat gcctgcacgt tggccatcgc
     2281 gcgcagcgtt ccgatgtact gcgaaaccac gagcagtatc acttgctctc gcgtcgagag
     2341 actgttcgcc ttgctcgaat tcaccgtgct ctgcgctgcc tgatatctcc gccacaacgt
     2401 caggtcaaag accggcgtcc cgaagctcgg acctgcggaa aacacttgat acggtcccaa
     2461 cgtcttggga aatccaggga acggcgtctt gcctccgaac tgggcctgca ggtttacttt
     2521 ctgcgcttcg tcggagatct tcgcattcgc ttgtggcaac agatcggctc gcgcaatgtt
     2581 cttatcttgt tcgctttgcg ctgcttgcaa aatcgcgatc tgcgccgtcg ggttctgttt
     2641 aagcgccagc cccaccgctt gatccagggt cagccgcagc acctgtgagg gcgcattccc
     2701 cggcaacgca ctctgtttgt ttgtgccttg tgcgtgcgcc cccgtgggcc ccaacatcaa
     2761 actgaaaaat accgtgtaaa aaataatgtg tgaggacttg cggcgttcct tctcgctgtt
     2821 gcacgtatct tgcctctctc gtgccacgat ttcgtgcacg atggcttggt tcaggcagcc
     2881 ttctgcgatc aactggccac cctgccggtg aatttctttc agcagcactt tgccggcggc
     2941 atcgatgaag ctcacgccgc agaggttcac atggaacttc tcgcgcggcg acgccgcgtg
     3001 caactcccgc cagcattgct ccagcgccgc cacccacggc cccgccaggc ggccctcgac
     3061 gctgaggaac atttttccgc gcttcttttc ggttgtaatt cttagcactg attccgtcct
     3121 gctcgccact tgtagatgca ttgcggtcac catccgatgc aaatatttgt taatgtgtaa
     3181 acgattcaaa aataaagact tacgtgtgaa ttgcagggtt tgaagattct agataatgtc
     3241 catatatggg cgttatatta aatatggtcc gatagtatat gaccatatat ggtctacctg
     3301 cctgcgggag tagcctcctc ctcgcagcaa cctacttcac cgacgggccc tcttcgcttt
     3361 gcatgtacga atcgacccgc aaccccagcg gcagtggttg cccggcgtcc agctccacca
     3421 gcgtctccag aatcttggtg tccacgtgtt cggaaggttc gtcggtccgg atattctttt
     3481 tccccagaat gcgtcccacg cgaatcacgc ggccccagaa tttgtgtgtt ccgtacgcct
     3541 cggccgtaac atacgcgcgc tgacccactc gcaacttgga gacgtcgctt tcgtcgatgt
     3601 ccagccgcac gcgcagaacg gaatcgtccg ccatcgtgat caccggcgaa tcgaattgcg
     3661 tcgagacgct ctccccgttg tgccgcagct tcctcaaaat cacgccgttg atcggtgcac
     3721 ggatatagct tttctccagg aacgcgcgcg cctcggcaag cttcgcccgc gccgtggcta
     3781 ccgccgcttc cgcgtgagcg cgatcttctt cccttgcttc tgcatcaagc agcgcaaaat
     3841 gctgagccgc ctgttcgtac tgcgcgcgcg cgacctggta cccccgctcg taacgttccg
     3901 cttcgtcccg tgaaatcacg ttgcggtcgg caagcccgcg ccgccgctcc gcttcgcttt
     3961 tggagttgtc cagcacggcc gcagccgcct gcatcgacgc gtgcgcttcc cggcgttcct
     4021 gcgttcgtgc gccgttcacc gtccgccgca attcggcttc ccgctgcgcc agctccgcct
     4081 ctgccgccgc aacgcgcgcg cggtaatcgt cattttcgat ttccgccagc acctgcccgc
     4141 gctggacgcg gtcgccttct tccacgtgca cagctttcag acgcccgctc agctccgagc
     4201 tcacgcgaat ttcttctgaa accgcctcga ctcgcccagg cgcggcgatc agcgtcgaac
     4261 cgaccgagct tgcctttggc ccggacgccg agacttcatg tccccggctc ctcaacagat
     4321 ttgccatcga caatacaaac aagactcctg ccacgagtaa tacggtattg gatcgtttca
     4381 tgatctggcc tcttcctctt caaatcgaaa gttggtggga aatcaacggc accgctgcat
     4441 ggacttcgag aaatgacgac gtttccatcg cgctcgtcac gctctgctcc tcccgggcga
     4501 ttcgtccatc ttcgagatga ataatccgat cgccgaacga caagacacgc gggtcgtggg
     4561 tcaccatcac caccgctcgg ccgtgtaccc gcgccagccg ctgcaacagt tcgatcacgg
     4621 ttcgtccgct cgtccaatcc aatgcggccg tcggttcgtc cgccagtaga atcggcggat
     4681 cacccgccag cgccctggcg atcgccacgc gctgcttttg cccgccactc aggtccgccg
     4741 gaaacgatcg cgccttctgc gccagcccca cttctgcaag aagttcttct cctcgttttt
     4801 tcgcctctcc ggcgcgaact ccgcgcacgt ccagcgccag cgcgacgttc tccgccgccg
     4861 tcagcgccgg aaacaggttg aatccctgaa agacgaagcc gatcccctcg cgccggattc
     4921 gcggcagttc gctttccggc agcgcagaca cgtctttacc cagcacctcc agcttccctt
     4981 cactcgcgcg cagaatgcac cccagtatgg aaagcaatgt cgttttcccg ctgccggaag
     5041 gccccatcag cagcgttagt tcccccgcgc gtacctccag atcgactccc tgcaacgccc
     5101 gcaactcggt cgcaccttcc ctataaatct tccctagtcc cttcgcccga atcacggtct
     5161 gcgcctgact tagcttcatg tctttcttct ttcttgtgtt ttctttttcg ttctttgttt
     5221 tgctttcaac ttttgacttt caactttcga cttctcatcc tcggaacacc atcgccggat
     5281 caatccgcgt caccttgttg atcgaaacca gcgccgccgc cacgcacatc aacaccgcga
     5341 gaaacagcat gccgatgctc atcgaccacg gcatcaggat cactgctcca cctttctcgc
     5401 tcgcccgcac gatgaagtac gccacgatca tcgccgcgac gtaacccagc accgcgctcc
     5461 acaccgcctg ctcgatcagc acgcgataca agtaccggtt cgttgcgccc atggccttca
     5521 gtgttccgta ctcccggatg tgatccatcg tcgccgcgta aatcgtctgc gccactaccg
     5581 ccacgccaac gattagcccc atcagcgccg ccgtcagtac cgccagtccc gcacccgtcg
     5641 taaacatcca ataaaaacgc gtcttccggc tgaattccgc cgtcgtgtaa acatccacat
     5701 ccgccagccg cgcgcgcagc ctctcttgca gctctctcgg cgaaacgcct gccgccgctt
     5761 tcaccaggat gtacgccgtg ctgaactcgc tcgaagacgg attcgtgtaa tccagcgaat
     5821 tcttgaacgt cgcatacacg aagggcgaag tcgtgaacga acggattccc cgcgtgaatc
     5881 ccaccacgcg cgcgcggtgg tagccgatct ccacgcgatc gccgatcttc gaaacgccca
     5941 gcttgtcgcg gtacagttca tccacaatca cggagtcttc ttgcttcaaa tcggcaacgc
     6001 tgccttgcac aacattccac ggttccccca gcccgctccc cggatgaaat ccgatcactt
     6061 gtatgttttc cactcggcca tcggtgcgct tccagcgtcc gaacgcctgc atgtacttct
     6121 cggccagtgc cactccaggc gttgaaagta cttgatagaa cttccgctcg gaaaaattgc
     6181 gcccggtatc gaaatatccg acgccatgaa aaaccaccca caggtccgca ttcgaacggt
     6241 cgatgttgtt cgatgtcgtg gtcgtgaacc cgaggaacag cccgaactga ataatgatca
     6301 gcaccagcgc aaacacgatg cccgtcagcg tcaccgcgaa tcgcactttg tcgtgaaaca
     6361 gattccgtct tgccagtaag cccatcaccc ttgctcttgc ccttcctacg taacaccaac
     6421 tctaaaagta acgctgctcg ttagatgaac agcgttaata ttttggcaaa aaaatttatg
     6481 ccgccccctg cagccccgcc accagcgtat ctaccgtcga atgaatcact ttgtccctcc
     6541 ccacccaggg aaacgtatag ttggtgatca gcagtgacgt gatcccgtgg atccccgccc
     6601 aaagcgtctg actcgccaat tccacgttca tggc
//
LOCUS       DASYVO010000169         3703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_18057, whole genome shotgun sequence.
ACCESSION   DASYVO010000169 DASYVO010000000
VERSION     DASYVO010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18057"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="VGR03_05740"
     CDS             <1..1319
                     /locus_tag="VGR03_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEV1993812.1"
                     /translation="EQAKKGSNEPIPQAQVALVALDPRTGEIKAIIGGRDYGQSQLNH
                     ALAHRQPGSVFKPFVYAAAFDNAVDGVQPVITPATTIDDEPTTFEFDGKEYTPNNYGE
                     KFMGRVTVRDALTNSLNVATVKVAELIGYGRVVQVARKMGLGMNIQPTPAVALGAYEM
                     TPIDVAAGYTTFATIGTRAEPQFLRIVVNAEGTPLEKFSPQTHLALDPRVAFLVDSLL
                     KDVLNKGTGAGVRARGFTLPAAGKTGTSRDGWFSGFTSNLLCVIWIGFDDNRDLGLPG
                     GAVAAPIWADFMTRATALAQYRDVKDFTMPDGVQSVLIDSDTLQLATANCPTTREEVY
                     VAGSAPTVYCEVHGGHNLLSTTGSILSRLFGGEPKAPQTDANGQPVTPYDPNRPPIAT
                     GQGPPDPSAQSGEKKKNPLQKIFGIFGGKKKEPDKAKPKPEKGDSP"
     gene            1316..2167
                     /locus_tag="VGR03_05745"
     CDS             1316..2167
                     /locus_tag="VGR03_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1993813.1"
                     /translation="MTRIKMAFPAIVLLLALSISSAGQTFVETPGTPVAPRAVQSPAQ
                     APAQSPAESSVIVVKPGLTEEQLADLYMARKEYREAAISYKRLADQNPRNPVYLNKLG
                     IALHQQAALGAALKYYERASKVDPSYADAQNNIGTIWYQRKKYGRAIKAYQRAINIRS
                     DMAVLYSNLAYAYFGDKKYQEAISSFRQALALDPQLFEHSSSRSGSILQDRSVGDRGR
                     FYFLLAKSFAEAGNLERCLIYLRKAKDEGYKELSAVKSDPSFAAVLKDPAIQEVLAPK
                     PPDSTQP"
     gene            2251..>3703
                     /locus_tag="VGR03_05750"
     CDS             2251..>3703
                     /locus_tag="VGR03_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1993814.1"
                     /translation="MGRPFISPRYLLLLFVVLIFGTTSVAAQQSASPPRAGSAAAYQQ
                     GIYLVFPFENAGASSRLDWLGEGLEELTIQRLSGAGEQVYSHAGRLVELERTGLSRSS
                     KLSRATMLHVAEDLDADFVVFGKFTASGTKLTIESRILKVSPARLLPALRETGTLDSL
                     MDLHSRLAWRMLSGSDRNYALSLAEFTKRQRPLRLDAFEHYIRGLLASEDDARLRELR
                     EAARLEPEWPEPGFALGEAYFARRDCDSALPWFARVPKAHDRYVEAVFATGVCQLLRD
                     HPDHAEEAFTSLEEALKNNMVSGADLPEILNDLAIARARQGKTGAAQSDLRRAAELDP
                     DEDDYAFNLGLLALQTNDPAAAAGYFREAAEREPDNPEDRALLILSLEKAGKKTEADQ
                     ERESANETFGPNGLPAIRLDAKNETVARLNRIKTELDVTSLRAEIELAESPASAAASA
                     SSDDTSVTRLRRGRQELSAGRADAAEKEFRAVLA"
BASE COUNT          762 a         1095 c         1121 g          725 t
ORIGIN      
        1 gcgagcaggc caaaaaggga agcaacgaac cgatcccgca ggcgcaagtc gcgcttgtcg
       61 cgctggaccc gcggactgga gagatcaagg cgatcattgg cgggcgcgat tacggccaga
      121 gccagctcaa tcacgcgctg gcgcatcgcc agcccggctc ggtgttcaaa ccgtttgttt
      181 atgccgcggc attcgacaat gccgttgacg gcgtgcagcc cgtcatcacg cccgccacga
      241 caatcgatga tgagccgacg acgtttgaat tcgacggcaa ggaatatacg ccgaacaatt
      301 atggagaaaa gttcatgggg cgcgtcacgg tgcgcgacgc gctgacgaat tctctcaacg
      361 tcgcgaccgt caaagtcgcg gaacttatcg gctatggccg ggtggtgcaa gtcgcacgga
      421 aaatgggact cggcatgaac attcagccga cgcctgctgt tgctctcggc gcgtacgaga
      481 tgacgccgat cgacgtcgcc gcgggataca cgacgtttgc caccatcggg acgcgtgcgg
      541 agccgcagtt tctgcgcatc gtcgttaacg cggagggcac gccgctcgaa aagttctcgc
      601 cgcaaacgca cctggcgctg gatccacgag tcgcatttct cgtcgacagc ctgctgaagg
      661 acgttttgaa caagggcacg ggcgcgggcg tccgcgcgcg cggcttcact ctgcccgccg
      721 cgggaaagac cgggacctcc cgcgacggat ggttctcggg gttcacttcg aatctcctct
      781 gcgtgatttg gattggtttc gatgacaatc gcgaccttgg actgcccggc ggcgcggtgg
      841 cggcaccgat ctgggcggac ttcatgacgc gcgccacggc gctcgcgcag taccgggacg
      901 tcaaggattt tacgatgccg gatggagtgc aatccgtgct catcgattct gataccctgc
      961 agcttgcaac ggccaactgt ccaacgaccc gggaagaggt ctatgtggca ggctcggcgc
     1021 cgaccgtata ctgcgaagtc cacgggggcc acaacctact atctactacg ggctccatcc
     1081 tctcccgact cttcgggggc gagcccaaag caccgcagac ggatgcaaat gggcagccgg
     1141 tcacacctta tgatcccaac cggccgccta ttgcaaccgg ccagggcccg cccgatcctt
     1201 cggcgcaatc cggcgaaaag aaaaagaatc ctttgcagaa gatttttggt atctttgggg
     1261 gtaagaagaa ggaacccgac aaggctaagc ctaagccgga gaagggagat tcgccatgac
     1321 caggatcaaa atggcgtttc cagcaatcgt tctcctactc gctctttcga tatcctccgc
     1381 gggacagact ttcgttgaga ctccgggtac gccggtggcg ccgcgagcgg tgcaatcgcc
     1441 ggcacaagcg ccagcacaat cgccggcaga gtcttccgtc atcgtcgtga agccggggct
     1501 gacggaagaa cagcttgccg atctctacat ggcgcgcaag gaatatcgag aggcggcaat
     1561 ttcctacaag cggcttgccg atcagaaccc ccggaatccg gtgtacctga ataagctcgg
     1621 gattgcgctg caccagcagg cggctttggg ggcggcgctg aagtattacg aacgtgcgtc
     1681 gaaggttgat ccttcctacg ccgatgccca gaacaacatc gggacaatct ggtatcagcg
     1741 gaagaaatac ggccgggcca tcaaggcgta tcagagagca atcaatattc gcagtgacat
     1801 ggccgtcctc tacagcaacc ttgcctatgc ctactttggc gacaaaaagt accaggaggc
     1861 catctcctca ttccggcaag cgctggcgtt ggatccgcag ctcttcgagc acagcagctc
     1921 aagaagcggg tcgattctcc aggaccgctc cgtgggtgac cgagggcgat tctatttcct
     1981 gctcgcaaag tccttcgcgg aagcgggtaa tttggaacgt tgcctgattt atctgcgaaa
     2041 ggccaaagac gaaggctaca aggagctgtc ggccgtgaaa tccgatccct cttttgcggc
     2101 ggtcttgaag gatcccgcga tacaagaggt tcttgctccc aagccccccg acagcacgca
     2161 accctgaacc agtttcgatt cgccgatttc aggctcgatc cgcacgtccg gcgcgaaacg
     2221 cgcccgtacg tctgttacaa tgcaacagac atggggcgcc ctttcatcag cccgcgttat
     2281 ctgcttttgc tctttgtcgt cctcattttt ggaactactt cggttgccgc gcaacaatct
     2341 gcttccccgc cgcgcgcggg atcggccgct gcttatcagc agggaatcta tctggtcttt
     2401 ccttttgaaa atgcgggagc atcttcacga ttggattggc ttggggaggg cctggaggag
     2461 ttgaccatcc aacggctgtc gggagccggc gagcaggttt attcgcacgc ggggcggctc
     2521 gtcgaactgg aacggaccgg cttatcacgc tcttcgaaac tcagccgcgc gaccatgctt
     2581 catgttgcgg aagatctcga cgcggacttt gtggtttttg gcaaattcac ggcgagcggg
     2641 acgaagctca ccattgagtc acggatctta aaggtgagtc cggcgcggtt gctgccggca
     2701 ttgcgggaaa ccggcacgtt ggattcgctg atggacctgc actcccgcct ggcatggcgg
     2761 atgctctctg gaagcgatcg caattatgcg ctgagccttg ccgagttcac gaaacgccag
     2821 cgtccgctgc gtctcgatgc gtttgaacac tacattcgcg gattgctggc cagcgaagat
     2881 gacgcgcgtt tgcgcgaact tcgggaagcg gcgcgcctgg agccggaatg gccggaaccc
     2941 ggcttcgcgc tgggtgaagc ctatttcgcg cggcgcgact gcgattccgc gctcccttgg
     3001 ttcgcgcggg tgccaaaagc tcatgaccgc tatgtggaag cggtcttcgc cacgggtgtt
     3061 tgccagcttc tccgggatca ccctgaccac gcggaggaag cgttcacttc gttggaggag
     3121 gccttgaaga acaacatggt ctccggagcg gatctgccag aaattctgaa tgatctggcg
     3181 attgcgcgcg cacggcaggg caagacgggt gcggcgcaat ccgatctccg ccgcgcggct
     3241 gagttggatc ctgacgaaga cgactatgca ttcaatcttg gtctgcttgc actacagacc
     3301 aatgatcccg ccgcagcggc aggttatttc cgcgaagcag cggaacgcga gccggataac
     3361 ccggaggacc gggcgctgct tatactgtcc ctggaaaaag ccggtaagaa gaccgaggcc
     3421 gaccaggaac gcgagtcggc gaacgaaact tttggcccca acggactccc tgcgattcgc
     3481 ctggacgcga agaacgaaac cgtggcgcga ctgaatcgca tcaagaccga gctcgatgta
     3541 acatctcttc gcgcggagat tgaattggcc gagtcccctg cgagcgcggc tgcttccgct
     3601 tcttcggatg acacgtccgt gacgcgcctt cgccgcggcc ggcaggaact ttccgctgga
     3661 cgcgccgatg cagcggagaa ggaatttcgt gctgttcttg ccg
//
LOCUS       DASYVO010000170         5259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_161266, whole genome shotgun sequence.
ACCESSION   DASYVO010000170 DASYVO010000000
VERSION     DASYVO010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161266"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1665)
                     /locus_tag="VGR03_05755"
     CDS             complement(94..1665)
                     /locus_tag="VGR03_05755"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1993815.1"
                     /translation="MKRCVKIVSWFISVLVVCSFRAEAQLPPAKKDPLSPAQSAQGKA
                     GCSTTEASSCAEAAAKILPIVMGSSPLEENLRRLTDEIGGRVTGTPEMAKAVAWSVAA
                     FRAAGVDVHTEKYSLPVTWSEGDTRLELLGPVKFQVRLKAEGWSPATPAGGIEASVVD
                     VGYGNEDDFARAGASVKGAILLVHSDTGSTWADLFNEYLRPPEIISRALKNGAVAILW
                     MGARERLLLYRHTNSLAGEIDRIPQAVLAREDAMRLARFVASYPGKVRVRLDMPNKIG
                     GPIEQENVVGEIRGYEKPEESVILGAHLDSWELGTGALDNGCNAALVIEAARALKATG
                     LLPRRSIRFVLFSGEEQGTIGSYEYVKAHHAELDKIRAMITFDSGIGRVTGYSFGGRR
                     DIEAGVREVLKPLESWGANNHTFDASFGTDNFDFMLEGVPTLVANQEEANYLPNYHAA
                     SDTLDKVDIRELKLHTVLAALTAWGIADRAEPLGKRLNRAELDVLIKETGLDQQLKLL
                     GYWDAWQSGQRGRKP"
     gene            complement(1804..2688)
                     /gene="folP"
                     /locus_tag="VGR03_05760"
     CDS             complement(1804..2688)
                     /gene="folP"
                     /locus_tag="VGR03_05760"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV1993816.1"
                     /translation="MPKRQKFRLKLPSGALVLGERTLVMGVLNVTPDSFSDGGTFLDR
                     DRAIEHALEMERAGADLLDIGGESTRPGSAGTSAKEELGRVLPVLQGLRGLLKIPVSI
                     DTQKAEVAQAALDAGAQIINDISGLNSDPRLAEVAARRGAPLILMHMRREPRTMQAGP
                     FARDVMKDVAQGLRESIAIARKAGVAKSQIVLDPGIGFGKSYAQNYELLVKLPQLTKL
                     GYPLLVGTSRKGFLGATLALNGKPAPLEKRIWGTAATVTASILNGAHIVRVHDVAEMV
                     QVARVADCVLDTKRASKN"
     gene            complement(2799..3107)
                     /locus_tag="VGR03_05765"
     CDS             complement(2799..3107)
                     /locus_tag="VGR03_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993817.1"
                     /translation="MGKQNSDNLQVTCPCCQAKLTIDPVFGVVLSHEAPPRSGPNIDL
                     TDAAKILDDQKRQREDKFQDSWMQEQNKEDILAKKFEEAMKKAKDAPAGKPIRDFDLD
                     "
     gene            complement(3232..5181)
                     /gene="ftsH"
                     /locus_tag="VGR03_05770"
     CDS             complement(3232..5181)
                     /gene="ftsH"
                     /locus_tag="VGR03_05770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV1993818.1"
                     /translation="MNSSSTAKTILFWVSIVFLGVMLWQLVSKNGQAQREDEPSYSEF
                     MGKVDAGEVKEVTLYLSPNSYELQGEYAKPANRKFHVTVFKESAPDLAKELREKAVQI
                     KVKEVHSGDWVLILLNAAPLILLVGFCLFLMRQMQAGGNKALSFGKSRARLLSAQQKK
                     ATFKDVAGTDEAKEELQEIIEFLKDPQKFQKLGGRIPKGVLLVGPPGTGKTLLARAIA
                     GEANVPFFSISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGAGL
                     GGGHDEREQTLNALLVEMDGFETNEGVILIAATNRPDVLDPALLRPGRFDRRVVVARP
                     DVKGREEILRVHTRKVPISDDVDLSIIARGTPGFSGADLANLVNEAALWAARQNRKFV
                     MMVDFEMSKDKVMMGVERKSMILSDEEKRNTAYHEAGHALVAAMTPGADPVHKVTIIP
                     RGMALGLTMQLPEDDKHTYTREYLETMLAVLMGGRSAEEIFLGHITTGAGNDIERATD
                     IARNMVCEWGMSELGPLAYGKKEEAIFLGREIAQHRDYSEDTAIQIDKEVKRIVNGGY
                     ESAKNLLSNNRETLERIAQALLEREVIDAIEVKLLMEGKPLPEKPRTPPTPPPQAAPS
                     TDPKVVRPELRPAPGFTRGENPAKA"
BASE COUNT          975 a         1616 c         1483 g         1185 t
ORIGIN      
        1 atctagtata ccgggaagct ccccacgata tctttgatgc gcactaagtg aaggctgcca
       61 cgggtaaaag gtcggcaatt catggattat gtcctagggt ttgcggcccc gctggccgct
      121 ctgccacgcg tcccaataac cgagcaattt cagttgctgg tcgaggccgg tctctttgat
      181 gaggacgtcg agttcggcac gattgaggcg cttgccgagc ggttccgcgc gatcggcgat
      241 gccccaggcc gtcagcgccg cgagcacggt gtgcaatttc aattcgcgaa tgtccacctt
      301 gtccagtgtg tccgaagcgg cgtgataatt cggcaggtaa ttggcctctt cctggttggc
      361 gacaagtgtt gggacgcctt ccagcatgaa gtcgaaattg tccgtgccga acgaggcgtc
      421 aaaggtgtga ttgttcgcgc cccaggattc cagcggcttg agaacttcgc gcacgcccgc
      481 ctcaatgtcg cggcgtccac cgaacgaata tcccgtcacg cggccgatgc cggagtcgaa
      541 tgtgatcatc gctctgattt tgtcgagttc cgcgtggtgg gcctttacat attcgtaaga
      601 accaatagtg ccctgttctt ctccgctgaa aagtacgaag cgaatcgagc gtcttggcaa
      661 aagccccgtc gccttgagcg cgcgcgccgc ctcaatgacc agcgccgcat tgcagccgtt
      721 atcgagcgcc ccggtgccca gttcccagga atccagatgt gcgcccagaa tgacgctttc
      781 ctccggcttc tcgtagccgc ggatttcgcc gaccacgttt tcctgctcga tgggcccgcc
      841 aattttattg ggcatatcca agcgcacgcg aacttttccc ggataggacg cgacgaaccg
      901 cgcaagccgc atcgcgtctt cgcgcgccaa gaccgcctgc ggaatcctgt cgatctcgcc
      961 ggccagcgaa tttgtgtgac gatagaggag cagcctttcc cgcgcgccca tccagagaat
     1021 cgccacagcg ccatttttca gcgcgcggct gataatttcc ggtgggcgta gatattcgtt
     1081 aaagaggtcg gcccatgttg atccagtatc gctgtggacc agcagaatcg cacctttgac
     1141 cgatgcgccc gctctggcga aatcatcttc attgccgtag cccacgtcga caacgctcgc
     1201 ttcaatcccg cccgccggcg tcgcgggaga ccagccttcc gctttcaacc gcacctggaa
     1261 tttcaccggg ccgaggagtt caagccgcgt gtccccttcg ctccaggtca ccggcagcga
     1321 gtacttctcc gtgtgcacat ctactcccgc cgcacgaaac gccgcaacgc tccaggcgac
     1381 ggctttcgcc atctccggcg tgccggtcac acgtccgccg atttcatctg tcagccgccg
     1441 caggttttct tccagcggcg acgaacccat cacaatgggc agaattttcg ccgcagcctc
     1501 cgcgcacgac gatgcctccg tcgtggagca acctgctttt ccctgggcgc tttgcgcagg
     1561 gctcaacggg tccttcttcg ccgggggcaa ttgcgcttct gctctgaagc tgcagacgac
     1621 gagcacagag ataaaccaac tgacaatttt cacgcaacgt ttcattaggg acggcctttc
     1681 ctaaaaaatc taccgaagag tgcgggctgg attataccgg aggagatgca gcctagcatt
     1741 gttccaaatg tttctccttg caggcgaagc ctgcccgggc acagccgaag agggctactg
     1801 cgtttaattc tttgacgccc gcttggtatc gagaacgcaa tcagccaccc gcgccacctg
     1861 caccatttcc gcgacgtcgt gcacgcggac gatatgcgcc ccattcagga tgctggcggt
     1921 aactgtcgcg gcggtgcccc agatgcgctt ctctagcggt gcaggcttgc cgttgagggc
     1981 aagcgtcgct ccaaggaagc ccttgcgcga tgtgcccacg agcagtggat agccgagctt
     2041 cgtcagttgc gggagtttta cgagcaattc gtagttctgc gcgtagcttt tgccaaagcc
     2101 gatgcccggg tcgagaacga tctgcgattt cgcgacgcct gctttgcgag cgatggcgat
     2161 ggactcgcgc agaccttgcg caacgtcctt cattacatct cgcgcaaagg gccccgcctg
     2221 catggtccgc ggctcacgac gcatgtgcat caggataagc ggcgcgccgc ggcgtgccgc
     2281 aacctcggca agccgagggt cgctattcag cccggaaatg tcattgatga tctgcgctcc
     2341 tgcgtcgagc gcggcttgcg ctacctctgc cttttgtgtg tcgatggaaa cgggtatttt
     2401 cagcagtcct cgaagtccct gcagcaccgg cagtacgcgc cccagctctt ctttcgcgga
     2461 agtgcccgca gaacccggcc gcgtggattc gccgccgatg tcaagcaagt ccgctccggc
     2521 acgctccatt tccagcgcat gttcaatcgc tcgatcccta tcgagaaatg tgccaccatc
     2581 ggaaaagctg tccggcgtga cgttcagcac gcccatgaca agagtgcgct cgcccagcac
     2641 gagcgcgccc gatggcagtt tcagccggaa tttttggcgt tttggcatcc agtcggttgg
     2701 cttcaagtct aacgcaagtg gtgagacggg tattcttcgc tgccgctctc acttttgcac
     2761 tcaatcagaa agaggacggg caggaaggcc cgtcccagct aatccaggtc gaaatcgcga
     2821 atgggtttgc ccgccggcgc gtccttggct ttcttcatcg cctcttcaaa ctttttcgcg
     2881 aggatgtctt ccttgttttg ttcctgcatc caggagtctt ggaatttgtc ctcgcgctgg
     2941 cgtttttggt cgtcgaggat ttttgcggca tcggtcaagt cgatgttcgg gccgctccgg
     3001 ggcggcgctt catgagacag cacaacgccg aagacaggat caatcgtgag cttcgcttgg
     3061 cagcatgggc aggttacctg aaggttgtcg ctgttctgct tccccataac cgcattctac
     3121 gcctttttgc caggaacggg cacggcatgc ggcactcctg agaaaaggac gcccgaaaaa
     3181 tgtaggggcg cagcatgctg cgccccaatt gacgatacct gcgattgagt tttacgcctt
     3241 agccggattc tccccacggg taaagcccgg tgccggacgc agttccggac gaaccacctt
     3301 cgggtcggtg ctcggcgcgg cctgaggtgg cggcgtcgga ggcgtgcgcg gcttttccgg
     3361 cagcggcttc ccttccatca acagtttcac ttcgatggcg tcgatcactt cacgttccag
     3421 cagtgcctgg gcaatgcgtt cgagcgtttc gcgattgttc gacaatagat tttttgcgct
     3481 ttcgtagccg ccattgacga tacgcttcac ttccttatcg atctggatgg cggtgtcttc
     3541 ggaatagtcg cggtgctgtg cgatttcgcg gcccaggaag atggcctcct ccttcttgcc
     3601 gtaggccagc ggtccgagtt cgctcatgcc ccattcacag accatgttgc gcgcgatatc
     3661 cgtggcgcgc tcgatgtcat tgcccgctcc tgtggtgatg tggccgagaa aaatctcctc
     3721 ggcggagcgg ccgcccatca ggacggcgag catggtttcc agatattccc tggtgtaggt
     3781 gtgcttgtcg tcttccggca gctgcatggt gagtcccagt gccattccgc ggggaatgat
     3841 tgtgactttg tgcacggggt cggcgcccgg cgtcatcgcc gcgacaagcg cgtggcccgc
     3901 ctcgtgatat gccgtgttcc tcttttcctc gtccgaaaga atcatcgact tgcgctcgac
     3961 gcccatcatg accttgtcct tggacatctc gaagtccacc atcatgacga acttgcggtt
     4021 ctggcgcgcg gcccacagtg ccgcttcgtt gaccaggttg gcgaggtccg caccggaaaa
     4081 accaggtgtg ccgcgcgcga taatcgacaa atcaacgtcg tcgctgatag ggaccttgcg
     4141 cgtgtgcacg cggagaatct cctcgcggcc cttcacgtca ggccgtgcca caaccacgcg
     4201 gcggtcgaag cggccgggcc gcaacagcgc cggatccaaa acgtcgggcc ggtttgtcgc
     4261 ggcgatcaag atcacgcctt cgtttgtttc aaagccgtcc atctcgacaa gcaacgcatt
     4321 tagtgtctgt tcgcgttcat cgtggccgcc gccgaggccg gcgccacgat gacggccgac
     4381 cgcatcaatt tcgtcgatga agatgatgca cggcgcattc tttttgcctt gttcaaaaag
     4441 atcacggacg cggctcgcgc cgacgccgac aaacatctcc acgaagtccg aaccggaaat
     4501 cgagaagaag ggaacgtttg cttcgccggc gatggcgcgc gcgagcaaag tcttgcccgt
     4561 gcctggaggt ccgacgagca atacgccttt cggaattcgg ccgccgagct tctgaaactt
     4621 ctgcggatct tttaggaact cgatgatttc ctgcagctct tctttggctt cgtcggtccc
     4681 cgcgacatcc ttgaaagtcg ctttcttctg ctgcgcggag agcagccgcg cgcgcgattt
     4741 gccgaagctg agcgccttat taccgccagc ctgcatctgc cgcatcagga aaaggcagaa
     4801 acccacgagg agaatcaggg gggccgcatt caacaggatc aaaacccagt cgccgctgtg
     4861 gacttccttc accttgattt gaacggcctt ctcccgcaat tcttttgcga gatctggcgc
     4921 ggattccttg aagaccgtga cgtgaaactt gcggttcgcg ggcttggcgt attctccctg
     4981 tagctcgtag ctgttcggtg agagatagag cgttacttcc ttcacctcgc ccgcgtcaac
     5041 cttccccatg aactcgctgt agctgggttc gtcttcgcgc tgggcctggc cgttcttgga
     5101 caccaattgc cagagcatca cgcccagaaa cacaatggag acccaaaaga gaattgtttt
     5161 tgccgtggag cttgaattca cgttgtcctc acaaattctg aaacctgccg gaagaaacta
     5221 aatttcgctc acttcttccg tcccctgctg cttttcctg
//
LOCUS       DASYVO010000171        16358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_2979, whole genome shotgun sequence.
ACCESSION   DASYVO010000171 DASYVO010000000
VERSION     DASYVO010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 16358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16358
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2979"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..783)
                     /locus_tag="VGR03_05775"
     CDS             complement(103..783)
                     /locus_tag="VGR03_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEV1993819.1"
                     /translation="MPELNTKPEPIEVEFARAAVVVVDMQNAFASAGGLLDVAGLDIS
                     AASQVVQAIGRVLDAARAAGIPVVYLQMGYKPDLSNSGGPNSPNWHKELALNVMNRRP
                     EFRGKLLTEGTWDFAVVDELKPRPEDLVVIKTRYNGFAGTTLDSQLRTRGIRYLFFAG
                     IATNVCVESTLRDAYFLDYWPILITDGAMPAGPPAAHEATLYNVENFFGWTLRSQEFL
                     AAVKSSCA"
     gene            complement(799..2130)
                     /locus_tag="VGR03_05780"
     CDS             complement(799..2130)
                     /locus_tag="VGR03_05780"
                     /inference="COORDINATES: protein motif:HMM:NF025888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993820.1"
                     /translation="MQTGEFFDSEIETMPPEQLRTLQEAKLGKQLDYLLARSPFYQDK
                     FRAAGIRPEHFRSLQDFANFPFTTKEELRESQIASPPLGRHAAVEMDSVIRIHSSTGT
                     TGRPSFVGVTQRDAEVWTRVTARSFYTQGIRPTDIVMHGASLTLFAGGLPVKDAIERI
                     GATFVPVGAGASAKLVLIARALRANTLHCTPSYAIYLADYVRREAGIEPRELGFEKIV
                     CGAEPGGGIPSVRAKIQAEWGARVTEGLGNADMAPIIFAECRDQSGMHFNGQEYVFVE
                     IIDPESGALLPLEPGVSGELVYTSLERECVPLLRFRTRDRVTILGTSCACGRTSFKLR
                     CVGRTDDMLIVLGVNVFPSAVKDVVAGFYPKTTGELQIVLERPGPGVQPPLKVIAEYA
                     GSPDLILLRKEVESRIKAVLNVSAAIEFVPAGTLPRFEMKGQLVKKLYEMP"
     gene            2562..3266
                     /gene="pcaH"
                     /locus_tag="VGR03_05785"
     CDS             2562..3266
                     /gene="pcaH"
                     /locus_tag="VGR03_05785"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEV1993821.1"
                     /translation="MTPIVGYRRPYPGTQPNHLHPLYLSSVKRAPTKPLIYLPHTLSE
                     VTGPSFGAETVDRKACDLTRQHSGPPLGERIIVSGRVLDEHARPVAHTLVEVWQANAA
                     GRYRHKIDQHDAPLDPNFTGCGHTLTDAEGRYRFVTIRPGAYPWGNHHNGWRPAHIHF
                     SLFGPAFATRLVTQMYFPGDALLPYDPIFNCTADEQARNHLISLFDWETTIPGQALGY
                     RFDIILSGREATPLES"
     gene            3268..3834
                     /gene="pcaG"
                     /locus_tag="VGR03_05790"
     CDS             3268..3834
                     /gene="pcaG"
                     /locus_tag="VGR03_05790"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEV1993822.1"
                     /translation="MNLHGTTSQTVGPFFTIGLNRLNRDVLAGAGVTGERVTIAGRVL
                     DGDGKPVPDALIEIWQANSHGKYAHPEDQQDKPLEAGFPGHGRIPTDDHGNFHFTTIK
                     PGRVPGPEGKEQAPHLAVSVFMRGLLRRLVTRIYFPDELENAADFVLNLVEPSRRATL
                     IAKKTGSGGALEWNVVLQGPEETVFFDC"
     gene            complement(3868..4962)
                     /locus_tag="VGR03_05795"
     CDS             complement(3868..4962)
                     /locus_tag="VGR03_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEV1993823.1"
                     /translation="MPAPYRADHVGSFLRPPELLEARRTAEPERLRAFEDQHILRLLD
                     TQKELGFEIFTDGELRRRNFMSDFTDAVQGFDLGDAVSRAWSAGVARDAAVSSVTGIV
                     TAKLRQARPLTGHELPFLKAHCPGAIKMTLPSATQFPAISFKRGVTDKVYQDHSALLW
                     DIVEIMKTELARLSEEGVNYIQIDAPRYSYYMDAKWRDWIRAEMKAEPDALLEESIRA
                     DNACFRAARRPGVTLAIHLCRGNNRSHWYAEGGYDAIAEKLFTTMDVDRFLLEYDDER
                     SGTFEPLRLVPRDKTVVLGLVSSKRPQLERGDELVQRIKEASRYFPLENLALSPQCGF
                     ASTMEGNLLTEDEQWAKLRLVVETSRRVWG"
     gene            5364..6635
                     /locus_tag="VGR03_05800"
     CDS             5364..6635
                     /locus_tag="VGR03_05800"
                     /inference="COORDINATES: protein motif:HMM:NF006580.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1993824.1"
                     /translation="MQRNGVLPPRSRRAFLQLSAAASAAVALRAVTEPMLAHAARISS
                     FHKDAVMIDSNENPLGPGEAAREAVAGIVSQGGRYCPWLTEDLESTIAELEGLKPEYV
                     RTFPGSSSPLHYAVLAYTSPAKSYVTADPGYEAGMMAAMVSGARIVKVGLTKSHAHDM
                     RAMLAAARDAGVFYICSPNNPTGTLTDHSDIEYLLENKPKKSILLIDEAYIHFSDAKS
                     TVDLVKADKDVVVLRTFSKIYGMAGLRCGFALGRPDLLAKLDAYTGWNSLPITAMIAA
                     MASLKDAQLVPQRKRINATIREAVFQWLDRQGYSYIPSQSNCFMLDVKRPAKDVIAAM
                     ALQNVFIGRVWPAMPTYARITVGTGPEMERFQAAFQHVMTGAAAGRVEQAPVMLSENV
                     DGAPRRATRTFGTRLAGYMAANSNRLPADVT"
     gene            6811..8079
                     /locus_tag="VGR03_05805"
     CDS             6811..8079
                     /locus_tag="VGR03_05805"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1993825.1"
                     /translation="MEVGRRLVFLARICLLIGLLLSAYFVIRQGVGAWYFRKGGPEAV
                     QSAIQWDSGNPQYFDALGSLMHFYADAGNPKDIVSAYQTATRLSSHNAYYWADLGAAY
                     DWAGRPDDALRAFEQARQLFPNSPDINWRLANFYVRAGKTSEALRALQKVLAGDNVAR
                     HHAFALATSATRDTNAILSEMLPVRADIYFEYLDFLIAAGKMDAAEMVWARVLERNVP
                     FELRQVFPYLDALIQHREPERAAETWSALAGRLPAQIHRRAGGRDLITNGSFELDILD
                     GGLDWRILPVEGAIVSLDSANAVDGARSLRIEFDGKHNLDFYHVLQYVPVKPDTRYRF
                     SGSMRVNGITTQSGPRFQIYDTYDMPILFLSTSHLVGISDWQAEQVEFKTGAATRLLV
                     IRVARPASRKLDNRIAGAVWIDDVRLESLE"
     gene            8184..10130
                     /locus_tag="VGR03_05810"
     CDS             8184..10130
                     /locus_tag="VGR03_05810"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEV1993826.1"
                     /translation="MSPTPGHPGNSPRVVERRPHSRWTPTSLVYVELGDGNGGFVVNI
                     SESGLAVQAAQVVHDHVLSSIRLKLPHSVHKIETGGQVAWRGETRKKLGIQFVDLPDA
                     ARSQIRQWISSEASLAASPQKINAIPHRKLGPVIVPMASESEDLASKPLAPHETAETR
                     KQYPISFAMATALGGSETSVEVSAPPVEIASPPAVALAAPVEIESPPAVASSTPVEIE
                     SPPAVVAAPPAVACAPPVEIAPPPAVVAAPPVVIPAQPVAALPVAVRIPRPELVPKDR
                     RPHPSSPTGAAAAVPGGTSWPKISPPPLRSSKLIEDSKSKLAVYPAADLMGLGAQRRP
                     WASRAAIGVLLVIAAFAAGMAVGRRGLNQAVENLEKLSPSGKTADQSPASSAPAQIAV
                     AGTPGPSSTANAPAQITVPSASLQLSTPRAAAQRSAPSSSAPAPKVSPDKGVTTPSPL
                     VQASGAQGRVMENVPSQIVASLPAKPQGGGATSAAPPVAQAQVSAPIESSARTAGVAQ
                     QENRPLVPAPPKNPEGVTGSVATSSHFRSIRIPAEVRSQKSRLGDTLQIGQLISGDQP
                     AYPPDAIRQRVDGTVKLHAIVGRDGAIQSVEPVSGPPLLVPPAMTTVRGWRYKPSFLG
                     DLPIEREEDITLVFQLRNQPGPAN"
     gene            complement(10299..11090)
                     /locus_tag="VGR03_05815"
     CDS             complement(10299..11090)
                     /locus_tag="VGR03_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993827.1"
                     /translation="MNLVSALSKCIERAKISRGVPGLIRALALLFLLLPGLAHARTGS
                     PGQEQRPLGSLSTIGEVYVNNSLAPAESTIFSGDTLQTSATGSATFSMSGKGSFKIAP
                     QSQLVFTGELQYVAELKSGMVVMSSFGGATDIILRAGNFTVAPVIQGEQSASKIAREP
                     DGSFSVACLDGSVGVIPLEGGAGRFLQSGQSVSISPEGQLVSPQVPAAPPAAPPQPSS
                     TTPVSGVKKKSNTGWILLGVAGAGAVGIGAAAGGHGGGGTPPVSP"
     gene            11176..12345
                     /locus_tag="VGR03_05820"
     CDS             11176..12345
                     /locus_tag="VGR03_05820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1993828.1"
                     /translation="MDASMRVLHIVKTAVGASWVYHQVRVLLGLGVQCTVALPSEHEG
                     WAPSYRQAGATILPVNVDFPARRPWRFFSTLAACRRVVREVRPDLIHSHHVGTTLMLR
                     LALGKRSAIPRIFQVPGPLHLEHGFFAGLDTRLAGPQDHWIAGCQWTYRKYQELGVDS
                     EHVFLSYPGTDLKPFSEVRTGRLRRELGISAEVPLVGMVSYIYAPKWFLGQARGLKGH
                     EDFMAALKLVREARPDVRGVIIGGSWGNANRYEERLRDLGATLCNGFLTFLGTRQDVP
                     ALYPDLDLAVVPSLSENVGGAVEPLLSGVPVVATRVGGLPDLIQDNETGWLIAPRNPQ
                     ELARAILDALKNREEARRRTIEGQAKARNLFDVERTGREVAAIYEKVLARAARPT"
     gene            12405..13646
                     /locus_tag="VGR03_05825"
     CDS             12405..13646
                     /locus_tag="VGR03_05825"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEV1993829.1"
                     /translation="MSLRRNFAWTLAGNVAYAACQWGILVAIAKLGTPTMLGQFALGL
                     AVSAPVFMLTNLQLRVVLATDARHEYRLGHYITLRIWGTAAGLLLMAVFVSLAGFRRE
                     TALVVLFVSLAKAAETFSDLLYGFWQKHERFDKIAMALSGRGIGSLAAVAGVLYMTHS
                     ISKSAAAMALYWTVWLVFYERRIAMNLMDGAASGEQLRSEWDTQKCRQLVVLALPLGV
                     VTLLVSLSANVPRYFIQHYLGEAALGYFAAMAYVLVAGNTVMAAMGQSASPRLARYFH
                     ADRPAYSRLLKNMVLLGAVLGLAGIGLALFFGRLFLRLLYHPEYAEYAPLFIWLMVAA
                     ALAYVSSMLGYGMTAARVFRAQVPLSLAATGITAVACWILIPPLGLTGAAYAVLIAAV
                     FSCAASAYIVSAALRSPLSGL"
     gene            13659..14789
                     /locus_tag="VGR03_05830"
     CDS             13659..14789
                     /locus_tag="VGR03_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV1993830.1"
                     /translation="MVQRQLRILHALGTLNPGGVETWLLQVLNCMDRDRLQFHFCTFG
                     PQAGLYDAQVEKLGGRIVRCPKGRNPWAFARRFRKILREGKYDVVHSHVHLFSGALLR
                     WAKAEGVPSRIAHSHTSLDEKHGTLARRYYRLLMKSWIERYATHGLAASRPAAVALFG
                     ENWEADGRVRVLHCGIDLSPFQGPIDRDEIRNELGLPLDAPVVGHVGRFDLQKNHPFL
                     LEISGEILKRRPEIHFLLVGDGRFRPEIEAKARAMGVSGNIHFAGIRTDVPRLMRGGM
                     DLFVFPSLWEGLPVTLIEAQAAGLRCIISETITTEANILPQQVIRLSLSTRPEEWAAV
                     ALDAVGRGRVKGEMTLSVLAQTDYCVERSTSILSSFYAKVGG"
     gene            14797..16233
                     /gene="wzy"
                     /locus_tag="VGR03_05835"
     CDS             14797..16233
                     /gene="wzy"
                     /locus_tag="VGR03_05835"
                     /inference="COORDINATES: protein motif:HMM:NF025654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HEV1993831.1"
                     /translation="MRPPKPILKPVFVAGWVCVALFAIWQIQLGAGDSSTALSAGILL
                     LATLVFSRYVLGLSFTTSPMLYLALLGLFHLGLVVPWALGIYDVVRITWFMPHGLSRA
                     IALIIYSIVAYQIGVIAALGRRGYQRDSPLVDGLSLENSEVFAAGCLLFLTGAIMFTV
                     GLIALDPLGYYRLTYTETFRLRAEFDPRFFGSGMTIAFIGLSLAVSGASKQRLRTIFL
                     GGGVWLLALFFWGFRGPALISGLLVCAVSLKKGVRFPRWFAWVAVAFLLIALPAVRLA
                     RDEPADERFFHMSSRDFNILDGPAEMGASIRPLVETADYIGPWNYRYGRTYLMAIKGV
                     VPNLALRWEAPATESTEDLPPGHWITLIADPWSYKNNGGIGFSAIAEPYMNFGTEGVV
                     AYFFLVAILLVWLEKVSLESSYALASWSLILGPLLWTTRNDFFNFFRPVVWGLLCLGA
                     VRAFSEGFAFIPRTGKPNRLELKTKTVK"
BASE COUNT         3044 a         4902 c         5060 g         3352 t
ORIGIN      
        1 ggtgttgtgt ccactttgat gcagacgatg tcctggcccc tcaggaaaag gccgtcggtg
       61 agggtcccgc cgacatcgat gtctaccgtg gacacgctgg tctcatgcgc aagatgattt
      121 gaccgcagcc aggaattcct gcgagcgcaa cgtccaaccg aagaaattct ccacgttgta
      181 cagcgtggcc tcgtgcgcgg cgggtggacc tgcgggcatg gctccatcgg tgatcagaat
      241 gggccagtaa tcgagaaaat aggcgtcgcg cagtgtggat tcgacgcata cgttggtggc
      301 gatccccgcg aagaataaat atcggatgcc gcgcgtgcgg agctgtgaat cgagagtcgt
      361 tcccgcaaag ccgttgtacc gtgtcttgat gacgacaagg tcttcggggc gcggcttgag
      421 ctcgtcaaca accgcaaaat cccaagtgcc ttccgtcaat agcttccctc tgaactccgg
      481 ccggcgattc atcacattca gggccagctc tttgtgccag ttgggagagt ttgggccgcc
      541 actgttgctc aaatcaggct tgtaacccat ttgcagataa acaaccggaa tcccggccgc
      601 tcttgccgcg tccagcaccc ggccaatggc ctggacgact tgtgacgcgg cagagatgtc
      661 caatccggcc acatccagca agccgcccgc actggcgaat gcattctgca tgtccacaac
      721 gaccaccgcc gctcgcgcaa attcgacctc gatgggttcc ggctttgtat tcagctcagg
      781 catgggccac gcaaatctct atggcatctc gtagagtttc tttaccagtt gccccttcat
      841 ctcaaatctc ggcaatgtgc cggcgggcac aaactcgatg gctgcggaca cgttgagcac
      901 ggccttgatc cggctttcca cctctttccg cagcaggatg aggtccggcg agccggcgta
      961 ttccgcgatg accttcagcg gaggctgcac accgggcccg ggccgctcga gcacgatctg
     1021 caattcaccc gtagtcttgg gatagaaacc cgccaccacg tctttgaccg ccgatgggaa
     1081 aacatttacg cccagaacga tcagcatatc gtcggtgcga ccgacacatc ttagcttaaa
     1141 gctggtgcgt ccgcatgcgc acgaggtccc cagaatcgtc acgcgatccc gggtgcgaaa
     1201 ccgcagcagg ggaacgcatt cccgttccaa actcgtgtat acgagttcgc cggacacgcc
     1261 cggctcaagg gggaggagag ccccgctctc tggatcgatg atttctacaa aaacatattc
     1321 ctgcccattg aagtgcatgc cggattgatc gcggcattcg gcgaaaatga tcggagccat
     1381 atccgcgttg cccagtcctt cggtcacccg ggctccccat tcggcctgaa tcttcgcgcg
     1441 caccgatgga atcccgccgc caggctccgc cccgcaaacg attttttcaa aacccaattc
     1501 gcggggctcg atgcccgcct cgcggcgaac gtaatccgcc aggtagattg cgtacgatgg
     1561 cgtgcaatgc aaggtgttgg cgcggagggc cctggcaatc agcacgagtt ttgccgaagc
     1621 gcctgcgcct acgggaacaa acgtggcgcc gatgcgctcg atggcatcct tcaccggcaa
     1681 gccgccggca aagagggtca ggctggcgcc gtgcatgacg atgtccgtcg gccggattcc
     1741 ctgcgtatag aaggagcggg ccgtcacgcg cgtccagacc tcggcgtccc gctgggtcac
     1801 accgacaaag ctcggccggc cggtggtgcc cgtggaagag tggatgcgga tgacgctgtc
     1861 catctccacg gccgcgtggc ggccgagagg cggggaggcg atctgacttt cccgcaattc
     1921 ttccttggtt gtaaagggaa agttggcgaa gtcctgaagc gacctgaaat gctcgggccg
     1981 gatgccggcc gcgcggaact tatcctggta gaagggagaa cgagcgagca ggtaatcgag
     2041 ttgtttcccg agcttggcct cctggagcgt gcgcagttgc tccgggggca tcgtctcgat
     2101 ttctgaatcg aagaattcgc cggtctgcat tggaagattg ggtgcccggg gttacggccg
     2161 gcgcgaggcc gcgcggatgg ccgttggccg tctccggtcc ggccgcaact ctaccatcct
     2221 cagcgcggcg gtttcagcag aaaggtccgg cgtgccggag ggtgctctcc ggaaaacggc
     2281 gcccggtccg gagaagaccg ctgggattct agaccggggc ggtcatcgag cgcagtccaa
     2341 gttccagttc ctgctcgacg atcgcatatt cgatcatcgg cttgacactt gcgccgcctc
     2401 tgcatagatt ccctctcgtg tccggccgcc tgcgtcccgg ccgcaggaag agatggagag
     2461 agaaattggc tcgttttcta tccctccacg aagtgagcgc cgagctggcc tgatcgcgca
     2521 gcgcggtttc gctggcgcgg tttgaagagg aggaacccag tatgacgccg atcgtgggct
     2581 accgtcggcc gtatccgggg acgcaaccga atcacctcca tcctctgtat ctatcctccg
     2641 tgaagcgcgc gccaacgaaa ccgctgatct atctgcccca cacgctttcg gaagtgaccg
     2701 gtccctcgtt cggtgcggag actgtggatc ggaaggcctg cgacctgacc cgccagcact
     2761 ccggaccgcc gctgggagag cgcatcatcg taagcgggcg ggtgcttgac gagcacgcgc
     2821 ggccggtggc gcatacgctg gtggaagtct ggcaggccaa tgctgcaggc cggtaccggc
     2881 acaagatcga tcagcacgac gcaccgctgg acccgaattt cacgggctgc gggcatacgc
     2941 tgaccgatgc cgagggccgc tatcgctttg tcaccatccg gccgggagca tacccctggg
     3001 gcaaccatca caacggctgg cggccggcgc acattcattt ctcgttgttc ggtccggcgt
     3061 tcgccacgcg tctggttacc cagatgtatt ttccgggcga tgcgctgctg ccctatgacc
     3121 cgatcttcaa ctgcacggct gatgagcagg cgcgcaacca cctgatttcg ctgttcgact
     3181 gggagactac cattccggga caggcgctgg gctaccgatt tgacatcatc ctcagcgggc
     3241 gcgaagccac gccgctggag tcatgagatg aatctgcatg ggacaacctc acaaaccgtc
     3301 ggcccgttct tcaccattgg tctcaaccgg ctgaatcggg acgtgctcgc gggcgcgggt
     3361 gtcaccgggg aacgcgtgac gatcgcgggg cgcgtgctcg atggcgatgg caagccagtc
     3421 ccggatgcgc tgattgagat ctggcaggcc aactcccacg ggaagtacgc tcacccggag
     3481 gatcagcagg acaaaccgct cgaagccggg ttcccggggc acgggcggat tccaaccgat
     3541 gaccacggaa atttccactt cacaacaata aaaccgggcc gggtgccggg gccggagggc
     3601 aaagagcagg cgccccacct ggcggtgtcg gtgttcatgc gcgggctgct gcggcgactc
     3661 gtgacgcgaa tctactttcc ggatgagctc gagaacgccg ccgattttgt gctgaatctc
     3721 gtggagccgt cacggcgcgc gacgctaatc gcgaaaaaga cgggaagcgg cggcgcgctc
     3781 gagtggaacg tggtcctcca gggtcccgaa gaaacagtgt tcttcgattg ttgagagcgc
     3841 ccgggctcac ctccaccttt tgatcgctca gccccacacg cggcgggacg tctccaccac
     3901 gagccgcagc ttggcccact gttcgtcttc ggtgagcaga tttccttcca tggtcgacgc
     3961 aaagccgcac tgcgggctga gcgcaaggtt ttccagggga aagtagcggg acgcttcctt
     4021 gatcctctgc accagctcat caccacgttc gagttggggc cgcttcgagc ttaccagccc
     4081 cagcacgacg gtcttgtcac gcggaaccag gcgcaacggt tcaaacgtac ccgaacgctc
     4141 gtcgtcgtat tccagcagga agcggtccac gtccatggtc gtgaacagct tctcggcgat
     4201 ggcatcgtag cccccctccg cgtaccagtg gctgcgattg ttgccccggc agaggtgtat
     4261 cgccagcgtg acgccggggc gtcgcgccgc gcggaagcac gcgttgtctg cccggatcga
     4321 ttcttccagc agtgcatccg gctcggcctt catctccgcg cggatccagt cgcgccactt
     4381 ggcgtccata tagtagctgt accgcggcgc atcgatctgg atgtaattca cgccctcttc
     4441 cgacaatcgc gccagctcgg tcttcataat ctcgacgatg tcccacagca gcgcggagtg
     4501 atcctggtac accttgtcag tgacgccccg cttgaaggag atggccggga actgcgtcgc
     4561 gctgggcagc gtcatcttga tcgctcccgg gcagtgcgcc tttagaaatg gcagctcgtg
     4621 tccggtcagt ggccgcgcct gacgcagctt tgcggtgacg atgccggtca cactgctcac
     4681 cgcagcgtcc cgggccaccc ccgcgctcca cgcgcgcgac acggcatcgc caaggtcgaa
     4741 gccttggact gcgtcggtga agtcgctcat aaaatttcgg cggcgcagct cgccgtcggt
     4801 gaatatctcg aaccccaact ccttctgcgt gtccagcaga cggaggatat gctggtcttc
     4861 gaaggcccgc aggcgctcgg gctcggccgt gcgccgcgcc tccagcagtt ccggcggccg
     4921 gagaaaactc cccacgtggt cagctcgata cggtgcgggc atatccttgc ttcccctctc
     4981 tggcttgagg cagaacccgg cgtctcgccg ggcgcgggca aactttatcg cacatcggcc
     5041 ctcgcccggt ggcagagtgt ataggtcagc cccacgacag ggtacaagtt ttcccggttc
     5101 actacttcat ttccggactg ctgcgggcgg gactttctcc acaatcgaac gcagacggca
     5161 gaatgcaacg ggtttcaacc agaactggaa tctagcgtgg gaccaggagg cggaggtttc
     5221 cttcggccgc caaggcgacc tcaggataga caagttctcc cgcaccggtc tcgcccggac
     5281 gatgtagggg caggcgcagg cattcgacca gacatcaacg ccgttgattg cagtacaatc
     5341 ggtgacgatc aggaggatac gcgatgcaaa gaaatggagt tcttccacct cgttcccgcc
     5401 gggcttttct gcaactgtct gctgccgctt ccgcggcggt tgctttgcgc gccgttaccg
     5461 agcccatgct cgcccatgcc gcgaggatca gttcgtttca caaagacgcg gtgatgattg
     5521 actcgaacga gaaccccctc ggccccgggg aagcggcccg ggaggccgtc gccgggattg
     5581 tgtcgcaggg cggacgctat tgcccctggt tgaccgagga tctggagagc accattgccg
     5641 aactggaagg cctgaagccg gagtacgtcc gaacctttcc cggatccagt tcgccgctgc
     5701 actacgccgt gctggcctac acttcgccgg cgaagagcta tgtgaccgcc gatcccggct
     5761 acgaggcggg gatgatggcg gcgatggttt ccggtgcgcg catcgtgaaa gtgggtctga
     5821 cgaagagcca tgcgcacgac atgagagcca tgctggcggc cgcgcgcgat gccggcgtgt
     5881 tctacatctg cagcccgaac aatccgacgg ggacgctgac cgaccactcg gacatcgagt
     5941 acctgctgga gaacaagccg aagaaatcca tcctcctgat cgacgaagcg tatatccatt
     6001 tttcggatgc caagtccacc gtcgatctgg tgaaggcgga caaagacgtg gtcgtgctgc
     6061 gcaccttctc aaaaatctac ggcatggccg gtctgcgctg cggatttgcg ctcgggcggc
     6121 cagaccttct ggccaagctg gacgcttaca caggctggaa ttcactgccc atcacggcga
     6181 tgatcgccgc catggcgagt ttgaaagatg cgcagcttgt gccgcagcgc aagcgcatca
     6241 atgccaccat ccgcgaggcg gttttccaat ggctcgatcg ccagggatac tcgtacattc
     6301 cgtcgcagtc caactgtttc atgctggatg taaaacggcc cgccaaggac gtcatcgccg
     6361 ctatggctct ccagaatgtg ttcatcgggc gcgtctggcc ggcgatgccg acctatgcgc
     6421 gcatcaccgt cggaacaggc ccggagatgg agcgcttcca agctgccttc cagcacgtga
     6481 tgacgggcgc cgcagccggg cgagtggaac aagcgcccgt gatgctgagc gagaatgtcg
     6541 atggcgcccc gcgccgcgca acgagaacgt tcggcacgcg actagccggt tatatggcgg
     6601 cgaacagcaa tcgcctgccg gcagacgtca cgtaagatct ccctgactgt gaaccaggga
     6661 ctggggagca gagtttttgc aagtcgcgaa tccccattgc tcacgatgaa atcagaagac
     6721 gattgtccag gccggtcggg tgtcgccgga tggctcttcg taactccggc gttgcgaaat
     6781 cgcactgaaa cgcccgtaag gcggtgaaac ttggaagtcg ggcgcaggct cgttttcctg
     6841 gctcgaattt gcctgctgat tggtctgctt ctttccgcat actttgtgat tcgacaaggg
     6901 gtgggtgcct ggtatttccg aaagggcggg cccgaggcgg ttcaatccgc gatccagtgg
     6961 gattcaggca acccgcagta cttcgacgcc ctgggaagtc tgatgcactt ctatgccgac
     7021 gctgggaatc ccaaggacat tgtttccgcg taccagaccg ctacgcgatt gagttcgcac
     7081 aacgcttact actgggcgga cctgggcgca gcctacgact gggccgggcg tcccgacgac
     7141 gccctgcgcg ccttcgaaca ggctcgtcag ctcttcccca attcgccgga cataaactgg
     7201 agattggcga atttttacgt tcgcgctggc aagacttcgg aagcactgcg cgccttgcag
     7261 aaagttctgg cgggggacaa cgtggcgcga caccacgctt ttgcgctggc caccagcgcg
     7321 acgcgtgaca ccaatgcaat tctgagcgag atgctgccgg ttcgcgcgga catctatttt
     7381 gagtatctgg attttctgat tgcagcaggc aagatggacg cggcggagat ggtttgggct
     7441 cgcgtgctcg aacggaatgt gccgttcgag ttgcgtcagg tattccctta cctcgatgcc
     7501 ctcatccagc accgcgagcc ggagcgcgcg gcagagacct ggtctgcgct ggccggacgg
     7561 ctcccggcac agatacacag acgcgccggc ggccgcgacc tgattaccaa cgggagtttt
     7621 gaactcgaca ttctcgatgg agggttggat tggcggatcc tgcccgtgga aggggctatt
     7681 gtgagcctgg attcggcgaa tgcggttgac ggcgcccgtt cgctgcgcat cgagttcgat
     7741 ggaaagcaca atctggactt ttaccatgtg ctccagtacg tcccggtcaa acccgacacc
     7801 cgctaccggt tttccggttc catgcgcgtg aacgggataa ccacccagag cgggccgaga
     7861 ttccaaattt acgacacgta cgacatgccg atactgtttt tgtcgacctc ccacctggtg
     7921 ggcatttccg attggcaggc ggagcaggtt gagttcaaga ccggcgccgc tacgcggctg
     7981 ctcgtcatcc gagtggcgcg gccggcgagc cgcaagctcg ataaccggat cgcgggcgcg
     8041 gtgtggatcg atgatgtgcg tcttgaatcg ctggagtagc caaaaagccg ccgctgccgc
     8101 tcggcttgaa gcagaggtag agcgggcgta tacttcatca gagcagggtt tattgcggtc
     8161 cccaactgca acaaggttga ccaatgtcac caaccccagg gcacccgggg aattcgccgc
     8221 gggtcgttga gcgccgtccc cactcgcgct ggacacccac gtcactggtg tacgtggagc
     8281 tgggcgacgg caacggcggg ttcgtcgtca atatcagcga gagcggactg gccgtgcagg
     8341 ccgctcaggt ggtgcacgat cacgtcctct cgagtatccg gttgaagctg ccgcactctg
     8401 tgcacaagat cgaaacgggc gggcaggttg cgtggcgggg tgagaccagg aaaaagttag
     8461 gcatacagtt cgtcgatctg cccgatgccg cacggagtca aatcagacag tggatttcct
     8521 cggaagcttc gctggctgca tctccgcaaa aaataaacgc aattccccac cgcaaattgg
     8581 gacctgtgat tgtgccgatg gccagcgagt ccgaggacct ggcttcgaag cccctggcgc
     8641 cacatgagac ggcggagacc cggaagcaat atccgatttc ttttgcaatg gccacggccc
     8701 tcggcgggag cgagacgtct gttgaggttt ccgcgccgcc ggtggaaatt gcatcgccac
     8761 cggccgtggc tctcgcggcg cccgtcgaga ttgaatcgcc accggccgta gcttcctcaa
     8821 cacccgtcga gattgaatcg ccgccggccg tagttgccgc gccgccggcc gtagcttgcg
     8881 cgccacccgt ggagattgca ccgccgccgg ccgtagttgc cgcgccgccg gttgtaattc
     8941 ctgcgcagcc agtggcagct ctcccagttg cggtccgcat tccgcgtccg gagttggtgc
     9001 cgaaagatcg gaggccgcat ccaagctccc caaccggcgc agctgccgcg gtacccgggg
     9061 gaacaagctg gcccaaaatt tccccacctc ccctgcggtc ttcgaaatta attgaagact
     9121 cgaaatcaaa actggcagtc tacccggcgg ccgacttgat gggactcggc gcgcagcgcc
     9181 ggccctgggc aagtcgcgcg gcaatcggcg ttctgctcgt gattgctgcg ttcgcggcag
     9241 ggatggcggt aggaagacgc ggcttgaatc aagcggttga aaatctcgag aagttgtcgc
     9301 cgagcgggaa gacggcggac cagagtccgg cctcgagcgc gccggcgcaa atcgcagtgg
     9361 cgggcacgcc gggtccaagt tcaacggcga atgcgccggc acaaattacc gttccgagtg
     9421 cgtcactcca gctttccacc ccgcgtgccg cggcgcagcg ttcggctcca tcctcttccg
     9481 ccccggcccc aaaggtctct ccagataagg gcgtcacgac gcccagtccg ctggttcagg
     9541 ccagtggcgc gcaaggccgc gtgatggaga atgttcccag ccaaattgtt gccagcttgc
     9601 ccgccaaacc ccagggcggt ggagccacca gcgccgcgcc accggtcgcg caggcacaag
     9661 tctccgcgcc gattgaatcg tcagcccgca cggctggcgt tgcgcagcaa gagaaccgcc
     9721 cgctcgttcc ggccccgccg aaaaacccgg agggcgtcac cgggtcggtc gcgaccagct
     9781 ctcattttcg ttccattcga atcccggccg aggtcaggtc ccagaaatcg cggctcgggg
     9841 ataccctgca gatcggtcaa ctcatctccg gcgatcagcc cgcgtacccc ccggatgcaa
     9901 tacggcaacg ggtcgatgga accgtcaagc ttcacgccat tgtcggccgc gacggagcca
     9961 ttcagagcgt tgagccggtg agcggcccgc cgctgctggt gccgccggcc atgaccaccg
    10021 tccggggatg gcgttataag ccatccttcc tgggcgatct gccgatcgag agagaagagg
    10081 acattaccct cgtgttccaa ctgcggaatc aacccggccc tgcaaattag gaattgcgcg
    10141 tccgctcccc gggaaatccg gtgtgctatc tgatggaagt tcaggagtga accgccaagg
    10201 acaggcatgt cccgcgcttg cccgagcaga ccgagacggc gaaaactgcg gatgttgtgc
    10261 agatgacggg aagcgccgcg ggcgatccct caaaagatct acgggctgac gggcggagtt
    10321 cctcccccac catggccgcc cgcggccgcg ccgatgccca cggctccggc gcctgccacg
    10381 ccgagaagaa tccatcctgt gttgctcttt ttcttaaccc ccgatacggg tgttgtcgaa
    10441 gaaggttgtg gtggtgcagc cggcggagcg gccggcacct gcggggagac caattggcct
    10501 tccggggaaa tgctcacgga ttgacccgac tggagaaagc ggccggcgcc tccttctagg
    10561 ggaatgacgc caacgctgcc atccaggcag gcgacagaga acgaaccatc gggctcgcgc
    10621 gcaattttcg atgccgactg ttctccctga atcaccgggg ccaccgtaaa atttccagcc
    10681 cgcaggatga tatccgtggc cccgccgaat gagctcatca ccaccattcc cgatttcaac
    10741 tccgcaacgt actgcagctc gccggtgaag acaagttgac tctgcggcgc gattttgaac
    10801 gagccctttc cgctcatcga aaatgtcgcg ctgccggttg cggaggtctg aagcgtgtcg
    10861 cctgagaaaa tcgtcgattc ggccggcgcg agcgaattgt tcacgtaaac ttccccgatc
    10921 gtgctcaagg aaccgagagg gcgttgttcc tgccccggtg acccggtccg agcgtgagcc
    10981 agcccgggca acaacaaaaa gagcagcgcg agcgcccgga tcaagccggg aactccgcgc
    11041 gaaattttag cgcgttcgat gcacttggac agggcggaga ccagattcaa cgagaccccc
    11101 ttcccgtgaa gatgttgagg aagactccga tctatctacc acctcccttc gaaattgtcc
    11161 agaagcggta ctagaatgga cgcctccatg agggtgttgc acatcgtcaa gaccgctgtg
    11221 ggggcgagct gggtctatca ccaggtgcgc gtgctgctgg gcctgggcgt gcaatgtacg
    11281 gtggcgctgc catccgaaca cgagggttgg gcccccagct atcggcaagc cggcgccacg
    11341 atacttcccg tcaatgtgga ttttcccgcg aggcgtccgt ggcggttttt ctcgactctc
    11401 gcggcttgcc ggcgagtggt cagggaagtt cgacccgatc tgattcattc gcatcacgtc
    11461 ggcaccacct tgatgctgcg cctcgctctg gggaagcgtt ctgccatccc gcgcattttt
    11521 caggtgccgg gcccgctcca tttggagcac ggtttcttcg ccggactgga tacacgcctg
    11581 gcgggtccgc aggaccactg gatcgcaggc tgtcagtgga cgtaccggaa ataccaagag
    11641 cttggagttg attccgaaca tgtttttcta tcctatcccg gcaccgatct gaaaccgttc
    11701 agcgaagtac gaaccgggcg cctgcgacgg gagctgggga tctcagccga ggttcccctg
    11761 gtcgggatgg tttcctatat ctacgccccg aaatggtttc tgggccaggc gcgcggccta
    11821 aagggccacg aggattttat ggccgcgctg aagctggtca gagaagcccg cccggacgtc
    11881 cgcggagtga tcatcggcgg gtcgtggggg aatgcgaacc ggtacgaaga acgcctgcga
    11941 gatcttgggg caacgctttg taatggtttc ctgactttcc tgggaacgcg gcaagacgtc
    12001 cccgcactct atcccgacct ggatctggcg gtggtaccgt cgctttctga aaatgtgggc
    12061 ggagcggtgg aaccgctttt aagcggcgtc cccgtcgtgg ccacgcgtgt gggaggattg
    12121 ccggatctca tacaagataa cgaaaccggc tggctgatcg cgccgcgcaa tccgcaggag
    12181 ctggcgcgcg ccatcctgga cgcgttgaag aacagggagg aagcccgccg ccgcaccatc
    12241 gagggacaag cgaaagcacg aaatcttttc gatgtggaaa ggaccgggcg agaggtcgcc
    12301 gccatctacg aaaaagtgct ggcgcgcgcg gcgcggccaa cttgacgctc atgcctggat
    12361 cggacgcaca agttgccgag gcgccctccg gtctgcccga gagtatgtct ctgcggagaa
    12421 attttgcctg gacgctcgcg ggaaacgtgg cgtacgcggc gtgtcagtgg ggaatcctgg
    12481 tggctatcgc caagctaggc accccgacga tgctcggcca gtttgcgttg ggtctggcgg
    12541 tttcggcgcc cgtgttcatg ctgaccaatc ttcaacttcg agtggtgctg gccaccgatg
    12601 cccggcacga atatcgtctc gggcactaca tcacgttgcg catctggggg actgccgccg
    12661 ggctcctgct gatggccgtg ttcgtctctc tggccggctt tcgccgcgag acggctctgg
    12721 tggttctttt tgtgagcctg gcgaaggcgg cggaaacttt cagcgatctt ctttatgggt
    12781 tttggcaaaa gcatgaacgg tttgacaaga tcgccatggc cctcagcggc cgcggaatcg
    12841 gctcgctcgc cgcggtcgcc ggagtgcttt acatgacgca cagcatttcc aagagtgccg
    12901 ctgcgatggc tctctattgg accgtgtggc tggtctttta tgaacggcgg atcgcgatga
    12961 acctgatgga cggcgccgct tcgggggagc aactgcggtc cgaatgggac acgcagaagt
    13021 gccggcagct tgttgtgctg gccctgccgt tgggcgtggt gacgctcctg gtctccctga
    13081 gcgccaacgt gccgcggtat ttcattcaac attatctggg cgaggcggcc ctcggctatt
    13141 ttgcagccat ggcctatgtt ctggtggccg gcaacacggt gatggcggcc atggggcaat
    13201 cggcgtcgcc ccgcctggcg cgttatttcc atgcggaccg cccggcgtat tcgcgattgc
    13261 tgaagaacat ggtgctgctt ggcgcagttc tggggctggc agggattggg ctggcgctat
    13321 ttttcggccg gctgttcttg agactgctct atcaccccga atacgcggag tatgcgcccc
    13381 tgtttatctg gctgatggtt gccgccgcct tggcctacgt ttcttccatg ttaggctacg
    13441 ggatgacggc ggcgcgcgta tttcgggctc aggttccgtt gtccctcgcc gccacgggca
    13501 tcaccgctgt ggcgtgctgg attttgatcc ctccgctggg gttgacgggg gccgcttatg
    13561 ccgtgctgat cgccgcggtg ttttcatgcg cggcgagcgc gtacatcgtt tccgcggcct
    13621 tgcgctctcc gctatccggg ctctgaggtg cgggtcgaat ggtccaaagg caattgcgaa
    13681 tcctgcacgc cctggggacg ttgaacccgg ggggcgtgga aacatggctg ctgcaagttt
    13741 tgaactgcat ggaccgggac cggctgcaat ttcatttctg cacctttgga ccgcaggccg
    13801 gcctgtatga cgctcaggtc gaaaaactcg gcggccggat tgtgcgatgt cccaagggac
    13861 ggaatccgtg ggctttcgct cgccgctttc ggaaaatact gcgcgagggc aaatatgatg
    13921 tggtgcatag ccacgtgcat ctgttttccg gggccttgct tcgctgggcg aaggccgaag
    13981 gggtgcccag ccgcatcgcg cacagccata ccagcctcga cgaaaagcat ggcacactcg
    14041 cgcgccgata ttatcggttg ctgatgaaat cgtggatcga gcgctatgcc acgcacggcc
    14101 tggcggcctc ccggcccgcc gctgtcgcat tgtttggcga aaactgggag gcggatggcc
    14161 gcgttcgagt tttgcattgc gggattgact tgtctccgtt tcaaggaccc attgatcgag
    14221 acgaaatcag aaatgagctc ggcctgccgc ttgacgcccc ggtggtgggg cacgttggcc
    14281 gcttcgatct ccagaagaat catccttttc tcttggagat ttccggcgag attctgaaga
    14341 ggcggccgga aattcatttt ctgctggtcg gcgatggacg cttccggcct gagatcgaag
    14401 ccaaagccag ggctatgggt gtctccggca acattcattt tgcggggatt cggactgacg
    14461 tgccccgcct gatgcgtgga gggatggacc tttttgtttt cccatcgctc tgggaaggtc
    14521 tgccagtcac tttgatcgag gcgcaagctg ccggactgcg ctgcatcatc tctgagacca
    14581 ttacgaccga agcgaatatt ctaccgcaac aagtcattcg gctttcttta tccacgagac
    14641 cggaggagtg ggcagcagtg gcgcttgatg ctgtcggacg ggggagagtc aaaggcgaaa
    14701 tgacactctc agtgctggcc caaaccgact actgcgttga gcggagcacc tcaattcttt
    14761 cgagctttta tgcaaaggta ggggggtagg agacacgtgc ggccccccaa accaatactg
    14821 aagccggtat ttgttgctgg gtgggtctgc gttgcgttgt ttgcgatctg gcagattcag
    14881 ctgggcgcgg gtgattcgtc cacagcactg agcgcgggca ttcttttgct ggccaccttg
    14941 gtgttctcca gatatgtcct tggtttgtct ttcacgacga gcccaatgct ttacctggct
    15001 ctgctgggcc tttttcacct tgggctcgtt gtgccgtggg cactcggcat ctacgacgtc
    15061 gtgcgaatca catggtttat gcctcatggg ctctcccggg cgattgcatt gatcatttat
    15121 tcgattgtgg cctaccagat tggtgtgatt gccgctcttg gtaggcgcgg gtaccagagg
    15181 gattcaccac tggtcgacgg cttgagtctg gaaaattcgg aggtcttcgc cgcaggttgt
    15241 ttgcttttcc tgaccggtgc aatcatgttt acagtcggac ttatcgcctt ggatcctctt
    15301 ggttattacc gcctcaccta taccgaaacg tttcggttac gggctgagtt tgatccaagg
    15361 ttctttggat caggcatgac gattgcgttt atcggattga gtttagctgt ttccggcgcg
    15421 tcgaagcagc ggctccggac tattttcctc ggggggggcg tctggctcct ggcgctgttt
    15481 ttttggggtt ttcggggacc tgcgcttatt tcgggcctgc tcgtatgcgc agtatcgctg
    15541 aagaagggag tcaggtttcc gagatggttt gcgtgggtgg ccgtcgcatt tctgctgatt
    15601 gcactgcccg cggtgcggct tgctcgtgac gagccggcgg atgagcgttt ttttcacatg
    15661 tcatcgagag atttcaacat cctggacggc ccggcagaaa tgggtgcgag catccgaccc
    15721 ctcgtcgaaa ccgctgacta catcggcccc tggaactacc ggtatggcag aacgtatttg
    15781 atggcaatta agggggttgt tccgaattta gcacttcgct gggaagctcc cgccacggag
    15841 tcgacggaag acttgcctcc tggtcattgg ataacgttga tcgccgatcc gtggagttat
    15901 aagaacaatg gtggaatcgg gttctcggct atcgcagaac cgtacatgaa tttcggaact
    15961 gaaggtgtgg tcgcgtactt cttcttagtc gctattctgc tcgtttggct agaaaaggtt
    16021 tcactcgaaa gctcgtatgc gcttgcgagc tggtccctta ttttgggccc actgctatgg
    16081 actacccgaa atgacttctt caatttcttc cggcctgttg tctggggtct cttgtgcctc
    16141 ggagccgtcc gtgctttttc ggaggggttt gctttcatcc ctcggacggg aaagcccaat
    16201 aggttggaac tgaagacaaa aaccgtaaaa tgacgggacc tcaaagagta caaaactgtg
    16261 cgtctcctag tcacaactga agcgcacgtc tcactcgcat cctcgggaga gatctatgcg
    16321 catggcccgg cgaactacgc gttctggtcg cgctacct
//
LOCUS       DASYVO010000172         5145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_48197, whole genome shotgun sequence.
ACCESSION   DASYVO010000172 DASYVO010000000
VERSION     DASYVO010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48197"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VGR03_05840"
     CDS             <1..606
                     /locus_tag="VGR03_05840"
                     /inference="COORDINATES: protein motif:HMM:NF015369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV1993832.1"
                     /translation="ECVLAYAMNNEPLPMLNGFPLRLVVPGYFATYWVKALSLVRVLD
                     KPDENFWMKTGYRIPDTPRGSTTPEEMKAGKVKTVPIGKMPVRSFIITPDGTGKVPAG
                     LPILARGIAFSGYGRIVKVDVSADDGKSWSAARLGEEYGPYSFRTWEFPWTPRHPGRY
                     QLAVRATDQAGNTQTDEGVWNPGGYLWNKIERQEMIVGAAS"
     gene            607..1014
                     /locus_tag="VGR03_05845"
     CDS             607..1014
                     /locus_tag="VGR03_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993833.1"
                     /translation="MQVKRLRAALFAPLALAAGILLAETQTGDYSLTNLGEVRQPALL
                     QQTDGAVYGVSAYPTYRPALAPGEDRRLVEDYCSSCHSIRYITMQPPLPAATWEAEVN
                     KMIKTFGQPIPEEVQPKIIKYLQTHYTPETRKK"
     gene            1185..1261
                     /locus_tag="VGR03_05850"
     tRNA            1185..1261
                     /locus_tag="VGR03_05850"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1219..1221,aa:Arg,seq:tct)
     gene            complement(1292..2107)
                     /locus_tag="VGR03_05855"
     CDS             complement(1292..2107)
                     /locus_tag="VGR03_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1993834.1"
                     /translation="MVEDFKWARKREPRQKAKDARLVTGATVKRALRTLKLLFHQAER
                     SGYAVKNPVVGVAMFREPLHSMRVISFEEQAAYLFRASQPLRDIASVMLDTGMRPEEV
                     FRLRSENIDFKQKTIFNPFGKTKAARRTIPMTDDVVSLLKGRVKELAKKETPFVFASP
                     YDVQKPIGSVKKAHKSAADRAKIKGHFRLYDLRHTFATRAVAAGADLPTLSAILGHAS
                     IQMTMRYVHPAAEQKRLAIKKFERSSGEGVIKAAAARQSHAAPTEVTTLEHVQ"
     gene            2621..>5145
                     /locus_tag="VGR03_05860"
     CDS             2621..>5145
                     /locus_tag="VGR03_05860"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HEV1993835.1"
                     /translation="MIRKLGIAAFSAGVLAFAFSAHLAYGQAVDTGWQTRALITQNID
                     ESKRVVLRGNTHPQANAANDRGSVADEFPMEHMLLQLRRSPQQEQALQKFIDELQAKD
                     SPNFHQWISAEEFGAMFGLAKQDRDTITGWLESQGFTVNVVYASGMVIDFSGTAGQVR
                     AAFQTEIHNLEIRGQKHVANMRDPQIPAALASVVSGVVSLHDFRPHPMHVLRNANPNF
                     TFTDSLGGTNYAVVPPDLATIYNLNPLFNAGYSGQGQTIVLIEDTDVFTPADWSTFRS
                     TFGLSAYASASFTSVHPAPSSGTNNCGSPGVISPNDAEAILDAEWASAAAPSAAIEMA
                     ACADTANTFGGLIALQNLINASSQPPAIMSISYGQCETVNGAAANAAYSSAYQQAVAE
                     GVSVFVAAGDSGAAGCDNSVAEATHGIGVNAFASTPYNVAVGGTDFSDTFSGTNSVYW
                     NSTNTPAFGSAISYIPEIPWNDSCASELLSIYLSGSPITYGPSGFCNDLFGSLFLTTV
                     AGGGGPSGCATGTPSTSGVVSGSCQGWAKPSWQSILGNPNDGVRDTPDISLFAADGLW
                     SHFYVFCWSNTAKGGAACTGNPPSSWSGAGGTSFASPIMAGIQALVNQKTGARQGNPN
                     PVYYQLAAAEYGSSGSSSCNSNNGNTVSAACIFYDVTLGDMDVDCVGSNNCFLDSSTE
                     GVLSTSNSSYQPAYGTNTGWDFATGIGSVNAANLVNNWPASTPPTLTTISPSSGQQGQ
                     TINNITLTGTNLSSSTITALNGITVSNTTSTATQVTATFAIAANAPLGAQNVRVTTAG
                     GTSNGVSFTVSAAPSAPTLGSISPSNGVQGGNPGVTLTGTNFVS"
BASE COUNT         1033 a         1636 c         1457 g         1019 t
ORIGIN      
        1 gaatgcgtgc tcgcgtacgc catgaacaac gagccgctgc ccatgctcaa tggatttccg
       61 ctgcggttgg tggtgcccgg ctacttcgcg acctactggg tgaaggcgct gagcttggtc
      121 cgcgtgctcg acaagcccga cgagaatttc tggatgaaga ccggctaccg catcccggat
      181 acaccgcgcg gctcgaccac gccggaagag atgaaagcag gcaaggtaaa aacggtgccc
      241 atcggcaaga tgcccgtgcg ctcgtttatc atcacgccag atggcacggg aaaggttccg
      301 gcaggcttgc cgattctggc gcgcggaatt gccttcagcg gatacggccg gatcgtgaag
      361 gtggatgttt cggccgacga cggaaaatcc tggagcgcgg cgcgtctcgg cgaggagtat
      421 ggtccttatt ctttccgcac ctgggaattc ccgtggacgc cgcggcatcc ggggcgctac
      481 cagcttgccg tccgcgccac cgatcaggcc ggcaacaccc agacagacga gggtgtgtgg
      541 aatcccggcg gatatctctg gaacaagatc gagcggcagg aaatgatcgt gggagcggcc
      601 agctaaatgc aagtgaagcg attgcgagcc gccctcttcg cgccgctggc gctggccgcc
      661 ggaattctgc tggccgaaac gcaaacgggc gactacagcc tgacgaatct cggcgaggtg
      721 cggcagcccg ccctgctcca gcaaacggac ggcgcagtct acggggtaag tgcctatcca
      781 acttaccggc cggctctcgc gccgggcgag gaccgccggc tggtcgaaga ctattgcagc
      841 agttgccatt cgatccgtta catcaccatg cagccgccgc ttcctgcggc cacatgggag
      901 gcggaagtga acaagatgat caagacgttt ggccagccca tccccgaaga agtccagccc
      961 aaaatcatca aatacctgca aacccactac acgcccgaaa ctcgcaagaa gtgacgaatg
     1021 atgagtgaca attggcgagt gcaccccgcc gctttgttaa tgtgtggcgc ggcagctctc
     1081 cgcgtgcccg gctctcctgc tggtcacgtg tcacctatcg ttcatcacgg ttactcgggc
     1141 acttctatgc ggcgggttcc cttgctagaa tgaaggaaat cggcgcgccc gtagctcagt
     1201 cggatagagc gtctgccttc taagcagagg gtcgggggtt cgaatccctc cgggcgcgcc
     1261 acacttttca atggcttagc agcctctcgc gtcactgcac gtgctccaat gtagtgactt
     1321 ctgtaggtgc cgcgtgactt tgtcttgccg cggcagcttt gatcacgccc tcgccgctag
     1381 acctttcaaa cttcttgatc gccagtcgct tctgttctgc agctgggtgc acataccgca
     1441 tggtcatctg aatactggcg tggccgagta tcgcgctcag cgtcggcaag tcagcgccag
     1501 cagccacggc gcgcgtcgca aacgtgtgac gcaaatcgta caatcggaaa tggcccttga
     1561 tcttggctct atcggcggct gatttgtgag cctttttgac gctgccgata ggtttttgga
     1621 cgtcgtaggg cgacgcaaaa acaaacggag tttctttctt tgcgagttct ttcactcgcc
     1681 ctttgagcaa ggacacaaca tcatccgtca tcggaattgt tcgccgcgcg gctttcgtct
     1741 tgccgaacgg attgaaaatc gtcttttgtt tgaagtcgat gttctcgctc cgtaagcgaa
     1801 acacttcctc aggccgcatg ccggtgtcga gcatgacgct tgcgatgtcg cggagcggtt
     1861 ggctcgctct aaacaagtag gcggcttgct cctcgaaact gatgacgcgc atggagtgga
     1921 gtggttcccg gaacatcgct acgcccacga ccgggttctt aaccgcatag ccgctgcgct
     1981 ctgcctggtg gaatagcagt ttcagggtcc taagtgctcg tttcaccgtc gcgccagtta
     2041 ctaaccgagc gtccttcgcc ttctgcctcg gctcgcgttt gcgcgcccac ttgaagtctt
     2101 cgaccatgtt gctcgtgatt tcatcgagat acttgccact gaaaaaacgc cctagcgcgc
     2161 cacttcatct tgttccccgt gtaggatccc ccaagcctga agacccgcca tcgaaaaaaa
     2221 tgaaacgact aggaaaacct ccgaagaagc acaaccgaaa acagcctgca agagcgcaat
     2281 ccagccgaca ggattgcgct gcacatctgc acaaagaagg tcgccgccca atggtcaggg
     2341 acttgctgat tccttgaaca gacaagactc ctgtccgagc actgtccttt cgaccagttg
     2401 atctctacgc cctgctccag tgttaatctt attgatcgta ggtcaatcat tccttaatgt
     2461 cggttcggtt aagaagcgcg ctccggcagg gtttcttagt catgaggacc caggctttcc
     2521 ggcctttcaa gaacaggacg attcagcggc tctctggccg ccgcgatgaa tcccctcgca
     2581 tggtagcccg gtaagagttt tcttcgatat agggagaccc atgatccgaa aactgggaat
     2641 cgccgccttc tccgccggtg tcttggcatt cgcgttcagc gcccacctcg cctacggaca
     2701 agcggttgac acggggtggc agacgcgcgc actcatcacc cagaacattg acgagagcaa
     2761 gcgcgtcgtg ctcaggggca atacgcatcc ccaagcgaat gcagcgaacg atcgtggctc
     2821 cgttgcggat gagttcccga tggagcacat gctgctccag ttgcgccgct ctccccagca
     2881 ggagcaggcg ctccagaaat tcatcgatga actccaagcg aaagactcgc caaacttcca
     2941 ccaatggatc agcgcggagg aattcggggc gatgttcggt ctggcaaagc aagatcgcga
     3001 cacaatcacg ggctggctcg agtcgcaggg ctttacggta aacgtggtct acgcaagcgg
     3061 gatggtcatc gatttttccg gcacggccgg ccaggtgcgc gcagccttcc agacggagat
     3121 ccacaacctg gagataagag gtcaaaagca tgttgccaac atgcgtgatc ctcagattcc
     3181 cgccgcgctc gcttccgtgg tgtccggcgt cgtctccctg catgacttcc gtccgcaccc
     3241 catgcacgtc ttgcgcaacg cgaaccccaa tttcaccttt actgattcgc ttggcggcac
     3301 caactatgcg gtggtgcctc ccgacctggc caccatttac aacctgaacc ctctcttcaa
     3361 tgcgggttac tccgggcaag gccagaccat tgtgctcatc gaagacactg acgtattcac
     3421 gcccgcggac tggagtacct tccgctcgac gttcggcctt tccgcctacg cctcggcctc
     3481 ctttacgagc gtccaccccg ctccctccag cggcaccaac aactgcggca gtcccggggt
     3541 catctctccg aatgacgcgg aagcgatcct ggacgcagag tgggccagcg ccgcggcacc
     3601 tagcgcggcc attgagatgg cggcctgtgc ggataccgcg aacacgttcg gcggtctgat
     3661 tgccctccag aacctgatca acgccagcag ccagccgccg gccatcatga gcatcagtta
     3721 cggccagtgc gagacagtaa atggcgcggc ggccaatgcg gcgtacagtt ccgcttatca
     3781 gcaggcggtc gcagagggtg tctccgtttt cgtcgccgcg ggcgatagcg gcgcggccgg
     3841 ctgcgataat agcgttgcgg aggccacgca cggcatcggt gtcaacgctt tcgcctccac
     3901 gccctataac gtcgcggttg gcggcacgga cttcagcgac accttctccg gcaccaacag
     3961 tgtttattgg aattcgacca acacgcccgc cttcggctcg gcgatttcct atattcccga
     4021 gatcccctgg aacgactcct gcgccagcga gctgctttct atctaccttt ccggctctcc
     4081 cataacctat ggcccaagcg gcttttgcaa tgacctcttt ggcagcctct tcctgaccac
     4141 ggttgcgggc ggcggcggcc caagcggctg cgctacgggg acgccctcca caagcggcgt
     4201 ggtgagtggg agctgccagg gatgggccaa gccctcgtgg cagtcgatcc tcggaaaccc
     4261 gaacgacggc gtgcgcgaca ctccggacat ttcgctgttt gcagccgacg gcctgtggag
     4321 tcacttctac gtgttctgct ggtcgaacac cgcgaagggc ggagccgcct gcacaggcaa
     4381 tcctcccagc agttggtccg gcgcgggcgg aacatccttc gcgtcgccca tcatggccgg
     4441 catccaggcg ctcgtgaacc agaagacggg ggcgcgccag ggaaatccca atcccgtcta
     4501 ttaccagctc gccgccgccg agtacggttc gagcggcagc agctcgtgca attccaacaa
     4561 tggcaacacc gtgtccgccg cctgtatttt ctacgacgtt accctgggcg acatggacgt
     4621 ggactgcgtg gggagcaata attgcttcct ggattcgagc actgagggcg tgctttccac
     4681 ctccaacagt tcctaccagc cggcctacgg caccaacacc ggctgggact tcgccaccgg
     4741 aatcggcagc gtcaacgccg ccaatctggt gaacaactgg ccggcgtcga cgccgccgac
     4801 actcaccacg atcagtccgt cgagcggcca gcagggccag accattaaca acatcaccct
     4861 cacgggcacg aacctctcca gctcaacgat cactgcgctg aatggcatca ccgtctccaa
     4921 taccacctcg acggcgaccc aggtgaccgc cacgtttgcc attgcggcga atgcaccgct
     4981 cggcgcgcaa aacgtcaggg tgaccaccgc cggcggaacc agcaacggcg tcagcttcac
     5041 ggtaagcgcc gctccgtcag cgccgacact cgggagcatt agtccgtcca acggcgtgca
     5101 gggcggaaac ccgggcgtca cattgaccgg caccaacttc gtctc
//
LOCUS       DASYVO010000173         4203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_3016, whole genome shotgun sequence.
ACCESSION   DASYVO010000173 DASYVO010000000
VERSION     DASYVO010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3016"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VGR03_05865"
     CDS             <1..1129
                     /locus_tag="VGR03_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993836.1"
                     /translation="DWQSLQLNLPMTSVRDLVVHENDLVVATFGRSFWILDDVTPLRQ
                     IDSHVAAADAWLFRPETAIRMRPGSDQGTPVPKDESLAPNPPEGAVLDYYLKDKALSP
                     VQLEIFDSEGKLVRRFASDDVLHKTNPNDVPLQMEWVRDPKPLLAEAGMHRFVWDLRY
                     ALPKSVRTSFWGPAGPMAVPGNYIVKLTANGKSSTQPLTIKLDPRVKTPLDALARQFG
                     LASKLAARLGEVSIALQQVGDLRKQIETRKKEAGGNTELLTALQEMEKKVEAAVEADS
                     DADFGLFGLAIPSKEHEPLSQVAAALTGLLMIVDSSDLGPAADAAAASARWEEAAQET
                     LARWAAFQKDDLASVNALLEKAKLRTLVLSPIISGDAPTAH"
     gene            1366..2019
                     /locus_tag="VGR03_05870"
     CDS             1366..2019
                     /locus_tag="VGR03_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEV1993837.1"
                     /translation="MASPFGAQAQQGGLNIVVLDPAHGGTDPGARGTGGIRESEIVLD
                     FAIQVRRALELQGFQVVQTRQGNENPSFDDRSATANAQRGAVFVTLHISSTGLPGTAR
                     VYVNSDLPPVAVANGLIPWERAQAPFLGLSRTFGDLVQGFLAQRFKGSPEAAQTASVR
                     QLRSTAAPAIAVEISSVTVEDRADLDRMAPGVADAIARGATAFKPSYIVPNRPGALP"
     gene            2016..2759
                     /locus_tag="VGR03_05875"
     CDS             2016..2759
                     /locus_tag="VGR03_05875"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEV1993838.1"
                     /translation="MTPRWKFLLIGGLVFIVLAAAIYFPILRRRVKRAARLQQQSEEQ
                     ARRELTQPIAINPGDPRVRTKLFWASDTIDSTLVPVTVDLPLSNDPVLRSKQVLNTLL
                     AGPVDAELRTLPPDALLLAFYLLPDGTAIADFSEALATSIPSGIVSEQLAVNSITRTL
                     EANVPQVQRLKILIHGQEVETLAGHLDLTGTFVVNTKPVQAPVKPAPTPASTDKPASP
                     AAGKAPAPAPGTLTPDKAPGKLAQPERKP"
     gene            2798..3535
                     /gene="rph"
                     /locus_tag="VGR03_05880"
     CDS             2798..3535
                     /gene="rph"
                     /locus_tag="VGR03_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEV1993839.1"
                     /translation="MRVDGRTADQMRPLKITPDFISTAEGSVLIELGNTRVICTATVD
                     DGVPGFLKGSGKGWVTSEYGMLPRATEERTPREATRGKQSGRTLEIQRLIGRSLRAVT
                     DQEALGERTVWLDCDVIQADGGTRTASITGAFVALALAFERLTAAGILKTSPLIDSVA
                     ATSVGIVDDRALLDLAYEEDSRAEVDMNVVMTGNGHFVEIQATAEGRAFSGSEMQDLL
                     ALAAAGIRRLTEEQRALLPVKFSARAR"
     gene            3519..4133
                     /gene="rdgB"
                     /locus_tag="VGR03_05885"
     CDS             3519..4133
                     /gene="rdgB"
                     /locus_tag="VGR03_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEV1993840.1"
                     /translation="MRALAKLFLASSNPGKLAEYRALAAASAPSLAVELVLLPDFEAL
                     PAFEENAATFAENAAGKALHYSRHGDGLVFADDSGLVVPALGGAPGIHSARYAGPQAT
                     NSQKIEKLLGEMREKKGSERVAYFVCAIALAARGRAMAIVTDRVDGEILEAPRGSGGF
                     GYDPVFYFPAIEKTFAELSAEEKNLRSHRGKAFRRLLAALSSML"
BASE COUNT          857 a         1166 c         1350 g          830 t
ORIGIN      
        1 cgattggcag tccttgcaac tgaatctgcc gatgacgtcc gtgcgcgacc tagtggtgca
       61 cgaaaatgat ttggtggttg ccacgtttgg gcggtcgttt tggatactgg acgatgtgac
      121 gccactgcgg cagatcgatt cgcacgtcgc cgccgccgat gcatggctct ttcgtccgga
      181 aaccgctatt cgcatgcggc cgggatcgga tcagggaact ccggttccga aggacgagtc
      241 gctggctccg aatccaccgg agggcgcagt gctagattat tatttgaagg acaaggcgtt
      301 atcgccggtc caactggaaa tcttcgactc ggaaggaaag ctggtgcggc ggttcgcgag
      361 cgatgacgtc ttgcacaaga caaatccaaa cgacgtgccg cttcaaatgg agtgggtgcg
      421 cgacccaaaa cctcttttgg ctgaggcggg aatgcaccga ttcgtctggg atcttcgtta
      481 cgcactgccg aagagcgttc gcacttcgtt ctggggaccg gccggaccaa tggcagtgcc
      541 gggcaactac attgtgaagc tcacggcgaa cggcaagagc agcacgcaac cgctgacgat
      601 caagctggat ccgcgcgtga aaactccgct agatgcgctg gcgcggcagt ttggcttggc
      661 ctccaaactc gcagcaagat tgggtgaagt ctcaatagcg ttgcaacagg tcggcgacct
      721 ccgaaagcaa atcgaaacac gaaaaaagga ggcggggggc aacaccgagc ttctgacggc
      781 cttgcaggag atggaaaaga aggttgaagc ggcggtggag gccgatagtg atgctgattt
      841 tgggctattt gggctcgcca ttccaagtaa ggagcatgaa cctctctccc aagtcgcggc
      901 ggcgctcacc ggtctcttga tgattgtgga cagctccgat ctggggcccg cggctgatgc
      961 tgcggccgca agcgcgcggt gggaagaagc tgcgcaggag acgctggcgc gttgggccgc
     1021 gttccaaaaa gacgatctgg cgagcgtcaa tgctttgctc gaaaaagcca aactgaggac
     1081 gctggtcctt tctccgataa tctccggaga tgcgccgaca gctcactagc gcttacacct
     1141 ttcgagcgac gcgtattgag ggacggattt ggtatgctgc ttcaggtgcg aaacatcctc
     1201 caaatcaggt ctttctctct gcttgggcta ctctccgtgg ctattctggc tgcggcgatg
     1261 gtgtcggcat ctcaaacttc ctcgcagacc caacaacccg cgactggcac gccacccccg
     1321 gcaactccct cagcaccctc ttcgcaggga ggagtggtgg gatcggtggc ttccccgttt
     1381 ggcgcgcaag ctcagcaagg cgggctcaac atcgtcgtgc ttgatccggc tcatggggga
     1441 acagacccag gcgcgcgagg cacgggcggc attcgcgaga gtgagatcgt gctggatttt
     1501 gcgattcagg ttcgccgcgc gctggagctg caaggatttc aagtggtgca gacgcggcag
     1561 gggaacgaga atccttcgtt tgacgaccgg tccgcaaccg ccaatgcgca gcgcggagcg
     1621 gtgtttgtga ccctgcacat ctcgtcgaca ggattgccgg gcacggcgcg tgtttacgtg
     1681 aattcggatt tgccgccggt tgcggttgcg aatggcttga tcccttggga acgcgcgcaa
     1741 gcgccgtttc tgggactgag caggacgttt ggcgatcttg tgcagggatt tctcgcgcag
     1801 cgattcaaag gctcgccgga ggccgcgcaa accgcgagcg tgcggcagct tcgctctacg
     1861 gcagcgcctg caatcgcagt ggaaatttcc agtgtgactg tcgaggaccg cgcggacttg
     1921 gaccgcatgg caccgggagt tgcggatgcc atcgcgcgtg gagctacggc tttcaagcct
     1981 tcctacattg ttccaaatag accgggagcg ctcccgtgac gccacgctgg aaattcctac
     2041 tgatcggcgg attggtcttt attgtgcttg ctgcggctat ttattttccg attcttcgca
     2101 gacgcgtgaa gcgcgccgcg aggctgcagc agcagagcga agaacaggcg cggcgcgaat
     2161 tgacgcaacc gatcgcgatc aatccgggcg atccccgcgt gaggaccaaa ctcttctggg
     2221 cttcggatac gattgattcc acgctcgtgc cagtaacggt cgatttgccg ctttcgaatg
     2281 acccggtgct gcgatcgaaa caggttttga acacgctgct cgcgggcccc gttgatgcag
     2341 aactgcgcac gctgccgccg gacgcgttgc tgcttgcgtt ttatctgctg ccggacggaa
     2401 cggcgatcgc ggatttttcc gaggcgctgg cgacttcgat tccctccggg atcgtgagtg
     2461 agcagttggc cgtgaattcc atcacgcgga cgctggaagc caatgtgccg caggtgcagc
     2521 ggctgaaaat cctgattcat gggcaggaag tggaaacact tgcggggcat ctggacttga
     2581 cggggacatt tgtcgtcaat accaagccgg tccaagctcc tgtgaagccc gcgccaacgc
     2641 cggcaagcac ggacaaaccg gcatcgccag cggctggcaa ggcgcccgct ccagcacccg
     2701 gaacgttgac accagataag gcacccggca aactggcgca gcccgagcgc aaaccgtaga
     2761 attcttgaac acgaaagtga tgaccgcagg aggaagagtg agagtcgacg gccggaccgc
     2821 ggaccagatg cgtcccttga aaatcacgcc ggattttatc tccacagccg aaggcagcgt
     2881 gctcattgag ttgggaaaca cgcgggtgat ctgcacggcc accgtggatg acggcgtgcc
     2941 cggttttttg aagggcagcg gcaaaggctg ggtcaccagt gaatatggaa tgctgccgcg
     3001 cgcgaccgag gagcgcacgc cgcgtgaggc aacgcgggga aaacagtcgg gacggacgct
     3061 ggaaattcaa cgattgatcg gccgctcgtt gcgcgcggtg acggatcagg aagcgctggg
     3121 cgagcgcacc gtatggctcg attgcgatgt gattcaggcg gatgggggaa cgcgcacggc
     3181 gtccatcaca ggagcgttcg tcgcgctggc gctagcattc gagcgactga ctgcggcggg
     3241 aatcttgaag acttcgccgc tgatcgactc cgtggcggcc acgagcgtcg gaatcgtgga
     3301 cgaccgcgcg ctgctggatc tcgcttatga agaggattcg cgcgccgaag tggatatgaa
     3361 cgtggtcatg accggcaacg ggcattttgt cgaaatccag gcgacggcgg aaggacgcgc
     3421 gttttccgga agcgagatgc aggacttgct ggcgctcgcc gccgcgggga ttcgccgctt
     3481 gacggaagaa caacgcgccc ttctgcccgt aaaattcagt gcgcgcgctc gctaagcttt
     3541 ttctcgcctc atccaacccg gggaagctcg ccgagtatcg cgcgctcgcc gcggcgtctg
     3601 cgccttccct tgcggttgaa ttggtattgc tgccggattt cgaggcgctt cccgcgttcg
     3661 aggaaaacgc tgcaaccttc gccgaaaatg cggcaggcaa agcgctgcat tactcgcggc
     3721 acggggacgg attggttttc gccgatgact cgggtctggt cgtgcctgcg ctcggcggtg
     3781 cgcccggaat tcattccgca agatacgctg gacctcaagc aacaaattcg caaaagattg
     3841 aaaagctgct gggcgagatg agagagaaga aaggatccga gcgtgttgcg tacttcgttt
     3901 gcgcgattgc actggcggcg cgtgggcggg ctatggccat cgtcacggat cgcgttgacg
     3961 gagaaatcct ggaggcaccg cgcggatcgg gcggatttgg ctacgatccg gtgttctact
     4021 tcccggcgat tgagaaaaca tttgcggagc tttctgctga agaaaagaat ctgcggagcc
     4081 atcgcgggaa ggctttccgc agattgctcg cggctctttc ctccatgcta tagttttggc
     4141 tcctatggtc acgaaatcga agaagaaaac atcgccgaag aagaaacccg ctccggtaaa
     4201 agc
//
LOCUS       DASYVO010000174         8684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_161405, whole genome shotgun sequence.
ACCESSION   DASYVO010000174 DASYVO010000000
VERSION     DASYVO010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8684
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161405"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..505
                     /locus_tag="VGR03_05890"
     CDS             185..505
                     /locus_tag="VGR03_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993841.1"
                     /translation="MPALSPQQTAILEKLLAKGFEFVSYPLYPNYFGVKKGNCAALLA
                     PQGSDAMRLFGEASYLVDGQLSVCVTRGAQALYVWKKKELAATPERAAEVDAFRRELL
                     AQLS"
     gene            860..1165
                     /locus_tag="VGR03_05895"
     CDS             860..1165
                     /locus_tag="VGR03_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993842.1"
                     /translation="MIQVTNPALLSAGPDGFAVDLTPLAKKPLLTRDEALVVRLHKVF
                     AAGGDTAGPYAVELSPAETARLGEALETLARARAWPPDVQRMNSDLRARLQSSPPAT"
     gene            2094..2321
                     /locus_tag="VGR03_05900"
     CDS             2094..2321
                     /locus_tag="VGR03_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993843.1"
                     /translation="MATARTRSHKHFRLDPVKIKRAQKVLRAGTETEAIDRALDLVIS
                     EHHRNRLALQAHERFVTSGIAIQDVYGTLEQ"
     gene            2322..2777
                     /locus_tag="VGR03_05905"
     CDS             2322..2777
                     /locus_tag="VGR03_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEV1993844.1"
                     /translation="MPAVLFDASVYITALRTKDDAGPSLRRLAEGLHLWLSSVVLEEL
                     YAGVSARHRRVVERLERDFDRVRRILVPNLRDWTQTGKVLARLAAKYGYEEIGKGRLT
                     NDALIAMSAGRLGITVITANARDFSRLAEFRPFAWRVNAPPTSEESVPC"
     gene            complement(3104..3448)
                     /locus_tag="VGR03_05910"
     CDS             complement(3104..3448)
                     /locus_tag="VGR03_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV1993845.1"
                     /translation="MILKIQEKRAEPAIVILEMAGRICLGRDCMDVEWRMDDLLKRGE
                     KKVIFDLTGIEFVDSTGVGILITCYGRLKKSGGELRLVGVQAKVQELLSMTQVDKVIA
                     AFPTIAAAAEKF"
     gene            3756..4667
                     /locus_tag="VGR03_05915"
     CDS             3756..4667
                     /locus_tag="VGR03_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993846.1"
                     /translation="MNRKVTMLSAIAVCLGAAAAAQAQQAMLTPPKVLVIGREVVKPG
                     KGAAHEKWEAGWPRAYARAKWPVNYLALTAMTGEGRALYITGYDSMAAWESDIQAQGK
                     NAAFTAETEALAEKDGEFLSENRTAVFTYMPELSYQPDVPVAGTRGFTIASFVLKPGH
                     ADQFTEARKAARVAHDKAGLTDHFAVYHLTAGGNTNTYLIFLPFKSLAEQDQFAAMHG
                     KAYQDALGEDGQKKLADLNSQAVESAETQLFAFSPKMSYVSKDWIAADPKFWAPKPAA
                     AAPAPPSGAAEKPAAKPAAKKEAPKKP"
     gene            4903..6615
                     /locus_tag="VGR03_05920"
     CDS             4903..6615
                     /locus_tag="VGR03_05920"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEV1993847.1"
                     /translation="MGFLAAVTTSGCGEGKSLPPPVISVSFPGGNSQTISLGQAVTIM
                     ASVSNDTSGRGVTWTLTGPGALSTQTTLSAEFVAPCGVTSNQIAMVTTTAVANAGASA
                     TFTVTVSPLPGLTNLSCDTFTNSTSQHATEVEPDTFAFGSTIVSTFQVGRYFTGASSD
                     VGFATSTDGGVTWTNGLLPGITVFQGGTFDAVGDPSVAYDAAHVQWIIATIAVHQDAS
                     GNPQTAQILVSRSPDGLNWGSPIAVNPLAFYDKPWIVCDDTSTSLFYGHCYLQWRDRG
                     LMVTSTSTDGGLTWPAPLKTADMINGLAGQPVVQPNGTVISPMVKLLFTHMLSFTSTD
                     GGASWSATTVISPITVHTEDGNMRSGAIPSAEIDGAGTVYVVWSDCRFRTNCSSNDLI
                     LSTSNDGVTWTSPARIPIDPLTSIADYFLPGLAVDRATSGNTAHLTLTYYYYPVSACG
                     TVCDLFVGYVSSQDGGQTWSTPVQLAGPMKTDWLANTSGGVMVGDYISTSYVNGQAFA
                     VFAVAAANNGSVFDEAMYTTTQPLLMSANPLRFSSEGEMPVPNAKSDHEPRDIYPPRR
                     PPLN"
     gene            complement(6663..>8684)
                     /locus_tag="VGR03_05925"
     CDS             complement(6663..>8684)
                     /locus_tag="VGR03_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV1993848.1"
                     /translation="GTVGYMSPEQVRGLPSDHRSDIFSLGAILYEILAGKRAFKGQTS
                     ADAMSAILKEDPPELSEINRAVPPAIDRIVRHCLEKNPEERFQSARDVAFGLESLSTV
                     SSTSAGVESAAGAARGARLRTPLLLVLPALVLVAAAAFWSGRITVPVSNPVFTRLTFR
                     SGYVASARFAPDGQTIVYSAGFGGNPLEIYSTRPGATQSRELGFKNSSILGISRADEM
                     AITLEHDFFVANASRGTLARVPFTGGAPREVAERVLCADWSPDGETLAVARESNGHIR
                     LEYPLGKVLYETGGWVSDVRVSPDGNRVAFIDHPIGWDSLGSIAMVDRAGKKTTLSGV
                     FSSALGLAWHPSGKEIWFTASQIHISSNLYAASLSGRQRLIWAGAGSLVLRDISRDGR
                     VLFVRENRRRGIGGLFPGHTTETDLSWQDWSLPTYLSPDGKWIYFAEEGDSAGAKYSA
                     YMRATDGSPAIRLGDGTPWGVSPDGKWVASLIPGEPQQLMLLPTRAGDAVNLSQPGFN
                     YDFVQWLPDGKRLLVGGNEPGKPSRHYLLNAAGGALKPVTPENQTGLLSPDGQSVVVA
                     LGDKIVFYPLDGGPPREAKNTLLGNVLPLTFRLNGRYFYASLRQEVPLKVYRLDVQTG
                     EKQFWKELAPGERAGVYNIGILSVTPDGRWYAYSYVRDFSDLYMVEGLK"
BASE COUNT         1783 a         2714 c         2596 g         1591 t
ORIGIN      
        1 tttgtgcccg aggggagtgc catccgtgac gcggattata ccgtgcaacg cgctgcttac
       61 acttccaatc tgaaccaccg gtgaaacgac tctgcccgcc ttctaaaacc ccctctaaaa
      121 ccccctctaa aaccccttca aaacaatgct ggacaggcgc ccacacgtat gctgagaatc
      181 tgacatgccg gcgctcagtc cacagcaaac tgccatcctg gaaaaactgc tggccaaagg
      241 attcgaattt gtcagctatc cgctgtaccc caactacttc ggcgtgaaaa aagggaactg
      301 cgccgcgctg ctggcgccgc agggaagcga cgcgatgcgc ctgttcggcg aagccagtta
      361 tctggtggac gggcagttga gcgtgtgcgt gacccgcggc gcccaggccc tctacgtgtg
      421 gaagaaaaaa gagctcgcgg cgacgcccga acgcgccgcc gaagtggacg cgtttcgccg
      481 cgagcttctg gcccagctct cgtaggggca cgacatgtcg tgccctctta tcttttgtcg
      541 ccggcgcctg taggggcgcg gcatgccgcg ccctcttatc tctgatcgcc ggttcatgca
      601 gggcgctgcc ccgaagcttc ggggctgcgc cctctgtagg ggggcgcttg ccccgccccg
      661 gccgcgtcgg cggcgaacgt cgaagcgggg cgcacttgta gcgctgccct ttagggcagc
      721 atcttcattc acctgtcgta gctccgccct gcaactgggg agatgggata tgctgccctg
      781 aagggcagcg ctacaacgag ggagaagaat tctgcgtctc gaatgcagcg gcgacgaact
      841 ggttctggct cttctgagcg tgatccaggt aacgaatccc gcgcttttga gtgccggtcc
      901 ggatggcttc gcggtggacc tcacgccgct cgcgaaaaaa ccgctactca cccgcgatga
      961 agcgctggtc gtacgcctgc acaaagtgtt cgccgctggc ggcgacaccg ccggacccta
     1021 tgccgtggag ttgagccccg cggaaaccgc gcggctcggc gaggcgctgg agactctggc
     1081 gcgggcgcgc gcctggcctc ccgatgttca gcgcatgaac agcgacctcc gcgcgcgctt
     1141 gcaatcctcc ccgccggcga cgtaacgatt ctgtcattct gagtcccggc cttgccggga
     1201 cgaagaatcc ctcttacatt ttttcgccac cacgatggag aggaagagag attcttcccg
     1261 ccgcggcggg ctcagaatga caaccacggg accggctcgt ggcggggtgg gccggcacgg
     1321 agaaatgccg ggttccatgg taaatattcc cccccgagga gaaattctcc ccctgcgggt
     1381 gcggcgtgcg acaacggagc aaaccgccgc gccataagtg gtacattacc cgcaagttga
     1441 agaaccgcga cgcggctccc tcttggtcat taaggcgcac tacaccggct tggccccaga
     1501 ggggcttcga aaaggagtac aacatggcgg cgaagaagac gcacaagaaa caaggcaaga
     1561 ggcgggcggc caagaaaatt gccagcaaga aaatgggcgc ggtccagact ttgtctagct
     1621 tcatcaggcg tggatagggg ctgaagtaaa gactcggcgc tcgagggtac ggctccctct
     1681 tgatcattaa ggtgctctgc gccgacttgg cctcagaggg gcttcagtta aggagaacaa
     1741 catgccggga aagaagagca agaagagcac aaggaagaac agggtgaaga agctggcggg
     1801 gaagaggttg gaaggggcca agacgctgcg caagtacccg ccaggtcccg tctaaaggag
     1861 acatggcagt gaagaagatg cacaagaagc agggccagag gcggccgggc aagaagcttg
     1921 cccacaagcg attgggagcg gtccagactt tgaaaccgcc cggcccccca cagcgggcct
     1981 gaagaaagac tcggcgctcg aatcgtgacg tcaccgtccc ggaatctgcg tgtgttcata
     2041 agttgttgtt ttgtgtacat ggaggcggta tcctcgcaca ggaggacagt tgcatggcca
     2101 ccgccaggac gcgatcccac aagcacttcc gtctggaccc ggtgaaaatc aagcgcgccc
     2161 agaaggtctt acgtgccggt accgaaacag aggccatcga ccgcgccctg gatttggtga
     2221 tttccgagca ccacaggaac cgcttggcgc tgcaagccca tgagcgcttc gtgaccagtg
     2281 gcattgctat tcaggatgtt tacgggacct tggagcagta gatgccggcg gtcctcttcg
     2341 atgcctcggt ctatataact gccttgcgaa cgaaggacga cgccggcccg agtctcaggc
     2401 ggttggccga aggcctgcac ctgtggttga gctccgtggt gcttgaagaa ctctatgccg
     2461 gggtgagcgc gcgccatcgc cgcgtcgtgg agcggctgga gcgcgatttc gacagggtca
     2521 ggcgaatcct ggtccccaac ctccgtgact ggacacaaac cggcaaagtg ctcgcccgcc
     2581 tcgccgcgaa gtacggctac gaggaaattg gcaaggggcg gctgaccaac gacgcgctga
     2641 ttgccatgag tgcaggccgt ttgggaataa ccgtgatcac tgccaacgcg cgtgatttta
     2701 gcaggcttgc agagttccgg ccctttgcct ggcgagtaaa cgctccgcca accagcgaag
     2761 agtcggttcc gtgctgatgc atcgagcccg cgaaacggac ttatccccaa tgagcttcgg
     2821 agaggagaac ggcatggcgg cgaaaaagat gcacaagaat cagagcaaga ggcgggcagg
     2881 caagaaactt gccagcaaga aattgggggc ggtcctgact ttgattccgc ccggcccccc
     2941 cgatagggtc taaagaaaga ctcggcgctc gaatcgtggc cgcaagactg gggcgttgct
     3001 gcgcgcggcc gtgtgagtgg cctccccatg ttcagcgcat gaacagcgac ctgcgcgcgc
     3061 gcttgcaagc ctggcgtcgc cgcagcccgg gaaatgaatc tgctcagaac ttttccgccg
     3121 cggccgcaat tgtcgggaag gcggcgatca ccttgtccac ctgggtcatg ctcagcagct
     3181 cctgcacctt ggcctgcaca cccaccagcc gcagttctcc tcccgatttc ttgagccgcc
     3241 cgtagcaggt aatcaggatg cccactcccg tgctgtccac gaactcgatc cccgtgaggt
     3301 cgaagataac cttcttctcg ccgcgcttga gcaggtcgtc catgcgccac tccacatcca
     3361 tgcaatcgcg ccccaggcaa atccgtccgg ccatctccag gatcacgatg gcgggctccg
     3421 cgcgcttttc ctgaatcttg agaatcatga aatttcctcg ctggcccagt tcgagcgccg
     3481 attcaagcgc caaatccgag cgccgaatcg attcaagcgc cgcatccgag tgccaaatat
     3541 tagagcgtgg ttcccgtcaa gtcaacgaac gcctgcccgg ccgctcaccg cctctgaatc
     3601 tcttgacatt ccggagaagc cggatagact ccgctcaccc tgggacccgg ctcgcgggcc
     3661 agcgcgcccg aatctctcat acgcgtttta gttgcccatt ttttcggcat cacaagagcg
     3721 tttttacgct aaacccactg aggaggattt acccgatgaa cagaaaagtc accatgcttt
     3781 ccgcaatagc agtctgcctg ggagcggccg ccgccgccca ggcgcaacag gcgatgctga
     3841 ctccgcccaa agtgctcgtc atcgggcgcg aagtcgtcaa acccggcaag ggggccgcgc
     3901 acgagaagtg ggaagccggc tggccgcgcg cctacgcccg ggcgaaatgg ccggtgaact
     3961 atctggccct caccgcgatg accggcgaag gccgcgcgct ttacatcacc ggatatgact
     4021 ccatggccgc gtgggaaagc gacatccagg cgcaggggaa aaacgctgcc ttcaccgcgg
     4081 aaacggaggc cctggccgag aaggacggag aatttctgag cgaaaaccgc accgccgtct
     4141 tcacctacat gcccgagctg agttaccagc ccgatgttcc ggtggcgggc acgcggggtt
     4201 tcaccattgc ttcgttcgtg ctcaagccgg gtcatgccga tcaattcacc gaggcgcgca
     4261 aggccgctcg cgtggcccac gataaggccg gcctcacaga tcattttgcc gtctaccatc
     4321 tcaccgccgg cggaaacacc aacacctacc tgattttcct gcctttcaag tcgctggccg
     4381 agcaggatca gttcgccgcc atgcacggca aggcgtacca agacgcgctc ggagaggacg
     4441 gccagaaaaa gttggcggac ctcaacagtc aagccgtgga gagcgccgag acgcaactct
     4501 ttgccttcag ccccaagatg agttacgtgt ccaaggattg gatcgccgcc gatccaaagt
     4561 tctgggcgcc caagcctgcc gccgcggcac cagccccgcc cagcggggcc gccgagaagc
     4621 ccgcggcaaa gcccgcggca aaaaaagaag cgccaaaaaa gccatagccg tcaggtcacg
     4681 acagaaaagg gacgggcggc agttccggcg gcgcgacgcg gaaacggtca tcaccggtaa
     4741 ccccaggaaa atgtttcacg aagaagagcg ctgggccgct tgccggcatc gttccgggag
     4801 tacacccccg gaacggtgct gacggctctg tgaattgcgg gccgcgaggg gagctttacg
     4861 acgcgcagac caggaaagat tgcttcgatc gccttcgggg cagtgggttt tctcgcagcg
     4921 gtgaccacta gcggctgcgg cgaaggtaag agcttgccac cgccggtcat ctccgtcagt
     4981 tttccaggtg gcaactcgca aacaatctcg ctgggtcagg ctgtcaccat catggcgagc
     5041 gtctccaatg acacctcggg gagaggcgtc acctggacac tcaccggccc gggcgctttg
     5101 agcacgcaga ccaccctgtc cgccgagttc gtcgctccat gcggcgtcac gagtaatcag
     5161 attgcgatgg tcacgaccac ggctgtagcc aatgcgggcg cgtcggctac gttcaccgtc
     5221 acggtctctc cccttccggg tctaacaaat ttaagttgtg acacattcac gaacagtacc
     5281 agccagcatg ccacggaagt ggaaccggat accttcgcct tcggttccac catcgtcagc
     5341 acgtttcagg ttggtcgcta cttcaccggc gcgtcgtcgg atgttggctt cgctacatcc
     5401 accgacgggg gcgttacctg gacgaatggt ctgcttcccg gaatcacagt tttccagggt
     5461 ggcaccttcg acgctgtcgg tgatccctcc gtcgcctacg acgcggcaca cgtccagtgg
     5521 attatcgcca cgattgccgt ccaccaagac gcttccggga atccccagac cgcacagatt
     5581 ctggtgagcc ggtctccgga tggcctcaat tggggaagtc ccatcgccgt gaatccgttg
     5641 gcgttttacg acaagccctg gattgtttgc gacgatacat ctacaagtct tttctacgga
     5701 cattgttatc tccagtggag ggacagaggt ctaatggtga cgagcacttc aaccgatggc
     5761 ggactaacct ggccagcgcc gctgaaaaca gcggacatga tcaacggact tgccggtcag
     5821 ccggtagtgc agccgaatgg caccgtgatc tcgccgatgg ttaagctact ttttacccat
     5881 atgctgtcgt tcacttcaac cgatgggggc gcgagctgga gcgccactac ggtgatctcc
     5941 ccgatcacgg ttcacaccga ggacggtaat atgcgcagcg gtgcgatccc atcggcggaa
     6001 atcgacggtg cgggcacggt atatgtagtc tggtcggact gccgcttccg caccaactgt
     6061 tcttccaacg atttgattct gagcacgtcc aatgatggag tgacttggac gtcgccggca
     6121 cggattccca tcgatccgct taccagcatt gcggactatt tcctgccggg gctggccgtg
     6181 gatcgcgcca cctcgggcaa caccgcccat ctcaccttga cctattacta ctatcccgtt
     6241 tcggcctgcg gaacagtttg cgatctgttt gtaggttacg tgtcgtcgca ggatggaggc
     6301 cagacctgga gcacccccgt gcagttggcc ggaccgatga aaacagactg gctggcgaac
     6361 acctcgggtg gtgtgatggt cggcgattac atctccactt cttacgtcaa cggacaggca
     6421 ttcgccgtct ttgctgtcgc ggcagccaac aatggctcag tttttgacga ggcgatgtac
     6481 acgacaacgc agcctctgct gatgtccgct aatccgctgc gtttcagctc ggagggcgag
     6541 atgccggtgc ccaacgccaa gtccgaccac gagcccaggg atatctaccc cccgcgcagg
     6601 cccccgctca actgatccag ccgcccgaga tggcgtcttc tgattcggtc tggctgccgg
     6661 cgttacttaa gcccttccac catgtacagg tcggagaagt cgcgcacgta ggaataggcg
     6721 taccaccggc catcgggagt gacgctcaga atgccgatgt tgtagacgcc cgcacgctct
     6781 cccggcgcca gttctttcca aaattgcttc tctcccgtct gcacgtcgag gcggtagacc
     6841 ttcagcggaa cctcctgccg aagtgaggcg tagaagtagc ggccattcag tcgaaacgtc
     6901 aggggaagca cgtttcccag caacgtattt tttgcttccc gcggcggccc gccgtccagc
     6961 ggataaaaaa caatcttgtc gccaagagcg acgacaaccg actggccgtc aggtgacaat
     7021 aatccggtct gattctctgg cgtaaccggc ttgagcgctc ccccggctgc attcagcaaa
     7081 taatgccgcg aaggcttgcc cggttcgttt ccgcccacca gcaggcgctt cccatccggc
     7141 agccactgca caaagtcgta gttgaatccc ggctgcgaga gatttacggc gtcacccgcc
     7201 cgcgtcggca agagcatgag ctgttgaggt tctccaggga taagcgaggc aacccacttg
     7261 ccgtcgggcg aaactcccca aggggttcca tcgccgaggc ggatggccgg cgacccgtcg
     7321 gtcgcgcgca tgtaggcgga gtacttcgct cccgcgctgt cgccttcttc ggcaaaatag
     7381 atccatttcc cgtcgggaga gaggtaggtg ggcagagacc agtcctgcca cgaaaggtcc
     7441 gtctccgtgg tgtgtcctgg gaacaggccg ccgattcctc ggcggcgatt ctcgcgaacg
     7501 aacaggacgc gcccgtcgcg tgagatgtct ctcagaacca ggctaccggc cccggcccag
     7561 atcaggcgct gccgcccgga caaagaagcg gcgtacaagt tactggaaat atgaatctgt
     7621 gatgcggtga accagatttc cttgccggag ggatgccagg ccagacccag cgcactcgaa
     7681 aataccccgc tcaatgtggt tttcttgccc gcacggtcca ccatggcgat actgccgaga
     7741 ctgtcccacc caatcggatg atcgatgaat gccaccctgt tcccatccgg cgacacgcgc
     7801 acgtcgctga cccagccacc ggtctcatac agcactttgc ccagtggata ctccaggcgt
     7861 atgtgaccat tgctttcacg ggccaccgcc agggtttccc cgtcgggtga ccagtcggcg
     7921 cagagaactc tctcggcaac ttcccggggt gcgccgcccg tgaacggcac gcgtgccagc
     7981 gttccccgcg aggcattggc gacgaagaag tcatgttcga gggtgatggc catctcgtcg
     8041 gcgcgcgaga ttcccaggat gctgctattc ttgaagccaa gctcgcggga ctgcgtggcc
     8101 cccggacgcg tgctgtagat ctccagcggg ttgccgccga agcccgcgct gtaaacaatg
     8161 gtctgcccgt cgggtgcaaa gcgcgccgat gccacgtacc cgctgcggaa ggtaagccgc
     8221 gtgaagacgg ggttggaaac cggcaccgtg atgcgcccgc tccagaacgc ggcggctgcc
     8281 accagaacaa gtgccggaag caccagcagc aatggcgtgc gcagacgcgc gccgcgcgcc
     8341 gctcccgcgg ctgactccac cccggcagaa gtagaggata cggtggagag cgattccagg
     8401 ccgaaggcca cgtcccgcgc cgattgaaaa cgctcttccg gatttttctc caggcagtgg
     8461 cgcacaatcc tgtcgatcgc cggaggcacg gcgcgattta tctccgaaag ctcgggagga
     8521 tcctctttga ggatcgcgct catggcgtcg gcggacgtct ggcctttgaa agcgcgcttg
     8581 ccggcgagaa tttcgtagag aatcgcgccc agcgaaaaaa tatccgagcg atgatcggac
     8641 ggcaggccgc gcacctgctc cggcgacatg tagcccaccg tgcc
//
LOCUS       DASYVO010000175         5987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_228437, whole genome shotgun sequence.
ACCESSION   DASYVO010000175 DASYVO010000000
VERSION     DASYVO010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228437"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="VGR03_05930"
     CDS             complement(<1..1139)
                     /locus_tag="VGR03_05930"
                     /inference="COORDINATES: protein motif:HMM:NF015688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HEV1993849.1"
                     /translation="MTDTKQPMESPSGLELHPDPPPTARISKKVGMAVLGVVVVVGVL
                     VVYGLYARREQQQVAQTKNEEKRPESARHIGDQLVKEMAPPAVANPAPVFSGRTGVDP
                     VQVNGGTQAHESGTLQPPGLVYRDDGKEGRSEARGAGDGSHSSTGGPSELSAEEKMRL
                     AAFRQEQEAFAAPTVVRGGSSLPGSSATGPLNDALQQFGSLASLLGGGKAPGSADGST
                     VIPVSARAQAGSDYDLQNEQDRKQVFIEKTRGRAVNNYLKSTRTPALGRYEVKMGWDI
                     PAILEQGINSDLPGEVKALVRSNVYDTATGKYLLIPQGSRMVGVYDSQIAYGQNRLQV
                     IWSRIVYPDGSSINLDGMMGQDIQGMAGFHDKVDNHYRRLVGSALL"
     gene            complement(1136..2317)
                     /gene="trbG"
                     /locus_tag="VGR03_05935"
     CDS             complement(1136..2317)
                     /gene="trbG"
                     /locus_tag="VGR03_05935"
                     /inference="COORDINATES: protein motif:HMM:NF015487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HEV1993850.1"
                     /translation="MKTNYVVAMLALSLVGSVAGQAQQATNGSATQSPTPAEPPPAVV
                     KREPVPSPAKYPLAEAVRALESGDAPGNGAAKHAPTVPALPANAAPAPVSPEVPRAFE
                     AKRDVALNATGVEAVVLSREWVESRNAPAPGKDGRVVYAYGGGLPIVVCAPLHVCILE
                     LEPGEKVLGEPHIGDSIRWEISPSVSGSGPDATPLIIIKPRIAGLDTTMVVPTDRRAY
                     YVRLESKPNEYVARVAFSYPEDTKQKWQEYLVKERETEQQAKAAAERVAELPNTALEN
                     MYWNYEIKGGDVSTRPVHVIDDGAKTYIQMPVETIHRELPVLVVKGPNGSEMVNYRVK
                     DNMYIVDQLFDRAALLLGSGKHQTKVELIRKVEVSGHKPHVQIPQAQPASSAAAAGAR
                     P"
     gene            complement(2326..3021)
                     /locus_tag="VGR03_05940"
     CDS             complement(2326..3021)
                     /locus_tag="VGR03_05940"
                     /inference="COORDINATES: protein motif:HMM:NF016238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VirB8/TrbF family protein"
                     /protein_id="HEV1993851.1"
                     /translation="MSKAIALTQQKSSKDESFGSPYLVARREWDERYGGLIKRAQQWR
                     GAAVLALLVALAEAVVIIGVATRPRTTPYVVAVDSLGRVAAAGTVDRTSPVDERMKLA
                     AITQWVQDMRGVTSDGLAERAAIDHVYAMIGSQSAAQTLVTDYFRANQPFERGSRETV
                     QVEVNAILPNSPHSYEVDWTETQRTLDGKPISSDKWRGIFTVAINPPTDEAVLRVNPF
                     GIYVMDITWSKVL"
     gene            complement(3039..4454)
                     /gene="trbL"
                     /locus_tag="VGR03_05945"
     CDS             complement(3039..4454)
                     /gene="trbL"
                     /locus_tag="VGR03_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HEV1993852.1"
                     /translation="MRGKRGGRRFCMVFSHVIVIGVVVLTAAALAHAQGPPSVNGPSS
                     ILDEYKGLQNGWITKLLGAAQRLFFLLAGIEVAWSFTLLAIEKADFQALTATIVRKIM
                     WIGIFYAILLYGVTPAGGGWIPAIINSFQILGQNASTVGPLGPSAIVGFGVNMAIDLL
                     SSLKAANFLTAFASSMALVFCAVVIFIAYLAVAIQFVVAMVESYLVIGAGVIFLGFGG
                     SRWTAAYVERYIAYAVSVGMKILVLYLLVGAGMTLSQGWIVVAKNAATSIDPARTGFD
                     LAAAAVMFLCVCWMSPKISSAMLGGVASMSGGDLAGVGMGVGMAAVGAGVLATGYAAR
                     GSDALTKVKEAVAAGSRGPGGGGANGATGATPRPGGGGGGNGNGPSSPSGGGSSAATQ
                     NGMPGGANQPAPPSRPLPAQTSGQQPGPPRSAAAGANDSFLGALERTATRAQYAMNSL
                     RIPHDGTSHAPPSSSTGHGES"
     gene            complement(4464..4787)
                     /locus_tag="VGR03_05950"
     CDS             complement(4464..4787)
                     /locus_tag="VGR03_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993853.1"
                     /translation="MKRAWMAVSILVLVVGFVGCKKAEPPSPIVKLMEDAGSGDVSKT
                     SDEGITRWLDQHKAVLLEVKKKCIEARASNPPAHWSDTTEGRVCADSYLLDPGIPLKT
                     DDKKY"
     gene            complement(4806..5516)
                     /gene="trbJ"
                     /locus_tag="VGR03_05955"
     CDS             complement(4806..5516)
                     /gene="trbJ"
                     /locus_tag="VGR03_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HEV1993854.1"
                     /translation="MKKVMVFTVVLSLLFLGVPVPTDAGVVAGVATEWTQILNHVQLI
                     MGYIRQGLQLQNELKQYAEQLKQGRVLPQQLFGPIQQDLSQLAQVVQGGQALAYSMGN
                     LDAQFRNTFKGYSFRTSGYFSDYKKWSQTSLDTTMNSLRAAGLQGQQLKNAQAVLAQL
                     RTMSTGAGGQMQAAEVGNQIAEQQVEELMSLRQLMLTDLQSKQAYQAEQIQKEQAESA
                     ALDQVFGTQPLVTDQKKY"
     gene            complement(5513..>5987)
                     /locus_tag="VGR03_05960"
     CDS             complement(5513..>5987)
                     /locus_tag="VGR03_05960"
                     /inference="COORDINATES: protein motif:HMM:NF040179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993855.1"
                     /translation="EYLRDWLKTLRKLNAVVVLATQNLSDIFNSGISDVVMEACPTKI
                     LLANAEAANVQSRRLYEAVGLNDREIQIVQGAIPKRQYYVVSPEGRRLISLGLGGVAL
                     AFVGVNGPDDRKKAEEVMRANPRTWQAEWLRQRGRADWADWWREVEGVLERRVVA"
BASE COUNT         1178 a         1922 c         1578 g         1309 t
ORIGIN      
        1 agtagggcag aaccaaccaa tcgtctgtag tgattgtcaa ctttgtcgtg aaacccagcc
       61 atgccttgaa tgtcctggcc catcattcca tcaagattga tcgagctgcc atcggggtaa
      121 acgatccgcg accagatgac ctgcaagcgg ttttgtccgt aagcgatctg ggaatcgtag
      181 actccaacca tccgcgatcc ttggggaatc aggagatatt ttcccgtcgc cgtgtcgtaa
      241 acgttcgagc ggaccaaagc ctttacctcg ccgggcagat ctgaattgat tccttgctcg
      301 agaatggccg gaatgtccca gcccattttc acctcgtatc tcccaagcgc cggagtgcgc
      361 gtggatttga gataattatt gacggcgcgt ccgcgcgtct tttcgataaa aacttgcttg
      421 cggtcttgtt cgttctgcag gtcatagtca ctgcccgctt gtgcccgcgc actcaccgga
      481 atcaccgtcg atccatccgc gcttcccggt gcttttccac ccccaaggag cgaagcgagc
      541 gacccaaatt gttgcagggc gtcattgagc ggccctgtcg cggatgaacc cggcaatgaa
      601 ctccctccgc gcacaaccgt gggcgcggcg aaggcttctt gctcctgtcg gaatgccgct
      661 aagcgcatct tctcttccgc cgataattcc gatggtccgc cagtggagga atgacttccg
      721 tcacccgcgc cacgggcttc tgaccgaccc tccttcccat cgtcgcgata cacgagcccc
      781 ggaggctgca aggtacctga ctcatgcgct tgcgttccgc catttacttg cacggggtcg
      841 acgccagtcc gtccggaaaa taccggagct ggatttgcca cggcgggcgg agccatctct
      901 tttaccaatt gatcgccgat gtgccgcgcg gactcagggc gcttttcctc attctttgtt
      961 tgcgccacct gttgctgctc ccggcgcgca tagagtccgt acaccacgag gaccccaacc
     1021 acgaccacga ctccgagcac cgccatgccc actttcttac taatgcgcgc cgttggcggt
     1081 gggtcaggat gtagctccaa ccccgaaggc gattccatcg gttgtttggt gtcggtcatg
     1141 gccgcgctcc ggctgccgca gcgctcgagg ctggctgcgc ttggggtatt tgcacgtgcg
     1201 gtttgtgccc gctcacttcc actttccgaa tcaactccac tttcgtttgg tgtttccccg
     1261 accccaacag gagcgcggca cggtcgaaaa gctgatcgac aatgtacatg ttgtctttca
     1321 cgcgataatt caccatctcc gaaccgttcg gccccttcac caccaggacg ggcagctcgc
     1381 ggtgaatcgt ctcgacaggc atctggatat aggtcttcgc accgtcatct atgacatgca
     1441 cgggccgcgt cgatacgtcg ccgcccttga tttcataatt ccagtacata ttttcgagcg
     1501 ccgtgttcgg caactcagcg acgcgctccg ctgcggcctt tgcctgctgt tcggtttcgc
     1561 gctcctttac aagatattcc tgccactttt gtttggtgtc ttccgggtag gagaaggcca
     1621 cgcgcgccac atattcattc ggcttcgatt ccaggcgaac gtaatacgcg cgccggtcgg
     1681 taggaaccac cattgtcgtg tccagcccgg caatgcgcgg cttgatgata atgagaggcg
     1741 ttgcgtccgg gccgcttccc gacacggacg gtgagatttc ccaccggatg ctgtcgccaa
     1801 tatgtggctc gccgagaact ttctctccgg gttccaattc caagatgcag acgtgcagcg
     1861 gcgcgcacac cacaatgggc aggcccccgc cgtaggcata caccacccgg ccatccttgc
     1921 cgggcgcagg agcattgcgg ctctccaccc attcgcggct caacaccacg gcctccacac
     1981 ctgtggcgtt gagcgccacg tcgcgcttcg cttcgaaggc gcgcggcact tccggtgaca
     2041 caggcgcagg agcggcgttc gcgggcagcg ccggcacggt cggcgcgtgc tttgccgcgc
     2101 cgttccctgg cgcatcgcct gattccaacg cccgcacagc ttccgccagc ggatacttgg
     2161 ccggcgacgg cacgggttct ctctttacca ccgcaggggg cggctcggcg ggtgttggac
     2221 tctgagttgc cgagccattc gttgcctgct gcgcttgccc cgccacgctt ccaaccaaag
     2281 aaagagccag cattgctact acatagttcg ttttcatagt gtccctcaca gaactttcga
     2341 ccacgtaata tccatgacgt agatcccgaa cggattcact ctcagaaccg cctcatccgt
     2401 tggaggattg atcgccaccg tgaaaatacc tctccacttg tccgaggaga tcggtttgcc
     2461 gtcgagcgtc cgctgcgttt ccgtccaatc gacttcatag ctgtgcggtg agttcggcag
     2521 gatcgcgttc acctccacct gcaccgtttc gcgggagccc cgctcgaacg gctgatttgc
     2581 acggaaatag tcggtgacga gtgtttgcgc cgcgctttga ctgccgatca tggcgtatac
     2641 atgatcgatc gccgcgcgct cggctaggcc gtcgctggtc acgccgcgca tgtcctgcac
     2701 ccattgcgtg attgccgcca gcttcatgcg ctcatcaacg ggagaagtcc gatcgacggt
     2761 acccgccgcg gccacacggc cgagcgaatc gacagccacc acatacggag tagttcgggg
     2821 gcgcgtcgcc acaccaataa tgacaacggc ctcagcgaga gccacgagta gcgccaggac
     2881 cgcagctccg cgccattgtt gcgcacgctt aatcaaaccg ccgtagcgtt cgtcccactc
     2941 ccggcgcgcg accaaatacg gactcccgaa ggactcatct ttcgagctct tctgctgtgt
     3001 gagtgcaatt gctttagaca ccgctccacc tctcgtagtt agctttcacc gtgccctgtc
     3061 gaactcgacg gtggcgcgtg agacgttccg tcgtgcggaa tgcgaaggct gttcatcgca
     3121 tactgcgccc gcgttgccgt acgctcgagc gcaccgagga aggaatcatt cgcaccagcc
     3181 gccgcggatc gcggcgggcc gggttgctgg cccgacgttt gcgctggaag aggacgcgac
     3241 ggcggcgcgg gttgatttgc gccacctggc atgccgtttt gcgttgcagc ggaagatcct
     3301 cccccactcg gggagcttgg tccgtttcca ttccccccac cgcctccacc tggccgtggt
     3361 gtcgcaccgg tggctccgtt cgccccaccg cctccaggtc cgcgtgagcc tgcggccact
     3421 gcttccttta ctttcgtcaa agcatcagag ccgcgtgcgg catatcctgt cgcaagtaca
     3481 cctgcaccca ctgcggccat gcctacaccc atgccaacgc ctgccaggtc gccgccgctc
     3541 atcgaagcca cgccgcccag cattgcggaa gagattttgg gagacatcca acaaacgcag
     3601 aggaacatta ccgcggcggc cgccaagtca aatccagttc gggcagggtc gatcgacgta
     3661 gcggcatttt ttgcgaccac tatccagccc tgagagagcg tcattcctgc tcccacaagc
     3721 aagtaaagaa cgaggatttt catgccgacc gacacggcgt aggcgatgta ccgctccaca
     3781 tacgccgcag tccaccgcga accgccgaat ccaagaaaaa tcactcccgc gccgataacg
     3841 agatagcttt ccaccatcgc caccacgaac tgaatcgcta cagcgagata cgcgatgaaa
     3901 ataacgacgg cgcagaagac caatgccatg ctgctcgcaa aggccgttag gaaattcgca
     3961 gcctttagcg acgaaagaag atcgatggcc atattcacac caaatccaac gatggcgctc
     4021 ggccccaatg ggcctaccgt cgatgcgttt tggccgagaa tttggaagct gttaatgatg
     4081 gcgggtatcc atccaccacc cgcaggggta acaccataga gcaggatggc gtagaaaatt
     4141 ccgatccaca tgattttgcg aacgatggtc gccgtgagcg cctggaaatc ggctttctcg
     4201 atggctagaa gggtgaagct ccacgctacc tcgatgccgg cgagtagaaa gaaaagccgc
     4261 tgcgcggcac ccagaagctt tgtgatccac ccgttttgaa ggcccttata ctcgtccagg
     4321 atgctcgatg ggccgtttac ggaaggaggt ccttgagcat gcgcgagagc ggcggcagtt
     4381 aacacaacaa caccaatcac aatcacatgg ctgaacacca tgcagaaccg ccgccctccg
     4441 cgcttgcccc tcatgtcctc acctcaatac ttcttgtcgt ctgtcttcaa gggaattccc
     4501 gggtctaata agtacgaatc cgcacacacc cggccctcgg ttgtgtcgct ccaatgcgcc
     4561 ggcgggttgg atgccctcgc ttcgatgcac ttttttttaa cttctaaaag aaccgctttg
     4621 tgttgatcga gccatcgagt aattccttca tccgaggttt tggacacatc acccgacccc
     4681 gcatcctcca tcagttttac gataggactt ggcggttccg ccttcttgca acccacgaat
     4741 ccaaccacaa gaactagaat cgaaaccgcc atccatgctc gtttcatgtc gtctcccctg
     4801 cgaagttagt attttttttg atcggtgacg agcggttgcg ttccaaatac ttggtccaga
     4861 gccgcactct ccgcttgttc cttctgaatc tgctccgctt ggtatgcttg tttactctga
     4921 aggtccgtca acatcaactg ccgaagtgac atcagttctt ccacctgttg ttcggcaatc
     4981 tgattcccca cctcagccgc ctgcatctgc ccgcctgcgc ccgtggacat cgtacgaagc
     5041 tgcgcaagca ccgcttgcgc atttttcagt tgctggcctt gcaagcccgc ggcccgcagg
     5101 gagttcatcg ttgtatcgag tgatgtctgc gaccatttct tgtagtcgct aaagtacccc
     5161 gaggtgcgga aactgtagcc cttgaacgta ttgcgaaact gcgcatcgag attgcccatc
     5221 gagtacgcca gggcttgacc gccttgcacc acttgcgcaa gctgcgatag gtcctgctgg
     5281 ataggaccaa agagctgctg cggtaacacc cgcccctgct tcaactgctc ggcgtattgc
     5341 ttcagttcgt tttgcaactg aagcccttgt cggatgtagc ccataatcag ttggacgtga
     5401 tttagaatct gcgtccattc tgttgctaca cccgccacta ctcccgcatc cgtaggaact
     5461 ggaacgccca aaaacaacag ggacagcaca accgtgaaca ccattacttt tttcatgcca
     5521 ctactctcct ttcgagaaca ccctcaactt cgcgccacca gtccgcccaa tcggcgcggc
     5581 ctcgttggcg caaccactcc gcttgccagg tgcggggatt ggcgcgcatg acttcctccg
     5641 cttttttacg gtcgtccgga ccgttcacgc cgacaaacgc aagcgcaacg ccgcccagtc
     5701 ccagcgaaat cagccgacga ccctctggcg aaaccacgta gtactggcgc ttcggaatcg
     5761 cgccctggac aatctggatc tcccggtcat tgaggcccac ggcttcatac aaacggcgcg
     5821 attggacgtt cgccgcttcc gcgttagcca gcaggatttt cgtggggcat gcttccatga
     5881 ccacatcgct gataccggaa ttgaagatgt cggagagatt ttgcgtggcg agaacgacca
     5941 ccgcgttcag cttgcgcaac gttttcagcc agtcgcgcag gtattcg
//
LOCUS       DASYVO010000176         3363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_174117, whole genome shotgun sequence.
ACCESSION   DASYVO010000176 DASYVO010000000
VERSION     DASYVO010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174117"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VGR03_05965"
     CDS             <1..643
                     /locus_tag="VGR03_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1993856.1"
                     /translation="PDLVLHGGDLAAGGTHPAEIIDQVRSLGWLGVRGNTDEMLWSPE
                     RLAEYAVANPKLAPILAMVQETIPPTLASIGEERLRWLEGLPSLYSQPGFSLVHASPD
                     DLWRAPMPNASDDELQSTYASLRAQIVIYGHIHCPYIRRLQGMTVANTGSVSQSYDAD
                     TRASYLVIDGENITIRRVEYDVESEAEELLRSGLPHADWLSRILLAGKYCPPE"
     gene            748..1152
                     /locus_tag="VGR03_05970"
     CDS             748..1152
                     /locus_tag="VGR03_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1993857.1"
                     /translation="MAIEIRGMAPLLQVFDMPRSIAFYRDVLGFEVVSTSTPRGEHFD
                     WALLRLNGVELMLNTAYEEDARPPVPDSARIAAHDDTCLYLGCPDVDAAYAHLRARGV
                     AAKEPKVAPYGMKQLYVSDPDGYNLCFQWPAK"
     gene            1363..2505
                     /locus_tag="VGR03_05975"
     CDS             1363..2505
                     /locus_tag="VGR03_05975"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEV1993858.1"
                     /translation="MREEEARVEEVNQFPAVERRRNPAVPSEQREKGEVAIFQELGKA
                     LTSSLELDQVLRTIMEKIDEFLRPDNWSLLLLDESKQELYFELAVGKASQALKDVRIK
                     MGQGIAGWVAQHGETVVVPDTAKDTRFFSKVDEKTKTETQSIVAVPVKFRDTCLGVIE
                     LINCIGPEGFDPRDLKLLEALSDFAAIALENARHVKKIHELTITDDCTSLYNARHMGF
                     ILETEIYRSQRYNYEFSIVFIDLDHFKQVNDTHGHLVGSRLLAEIGNALKEHCRLIDY
                     AFRYGGDEFVILLPQTSKENALNVARRLHKLVRERTWLVPEELNIRLTPSVGVASYPA
                     DSRTKEGLLHLADEAMYLVKNTRRDSVAAANMGILPNPVASAESAG"
     gene            2575..>3363
                     /gene="lptC"
                     /locus_tag="VGR03_05980"
     CDS             2575..>3363
                     /gene="lptC"
                     /locus_tag="VGR03_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEV1993859.1"
                     /translation="MKRSEAAKYARWSAAAALLLASLTAGVYLERKWVAYREKQKAPP
                     PAPQDVTRSSNGLTFSKMAGNQKIFTVEASKATDFKDKAASLLEDVKITIFGKTGERH
                     DTIHTQSCQYEKLGGSIACSGEVQLDLESRAEAERAAKNPEIAAQQKVHVETRGVVFN
                     RASGLARTEQPVKFVFPNGNGEAVGVEYHSEEGTVRLLRDVQFSLMPPRNDSAGKKTA
                     SAGAKEPIRVTGKSLDFERDSRTMQLDGPVEAETHTARLRAGKFT"
BASE COUNT          747 a          880 c         1091 g          645 t
ORIGIN      
        1 acctgacctc gttctccatg gcggcgattt ggctgccggt gggactcacc cggcggagat
       61 catcgatcag gtccgctccc taggatggct cggcgtccgc gggaataccg atgagatgct
      121 ttggtccccg gaacgtctgg ctgagtatgc cgtcgccaac ccgaagctcg cgccaattct
      181 tgccatggtc caagagacga ttcccccgac gctcgcaagc atcggtgaag aacgcttgcg
      241 atggctggaa ggacttcctt ctctgtactc gcagcccggc tttagcctgg ttcatgccag
      301 ccccgatgac ctgtggcggg ctcccatgcc caatgcgagc gatgacgaat tgcaaagtac
      361 ctatgcgtct cttcgcgctc agatcgtgat ctacggacat attcattgtc cgtacatacg
      421 ccggcttcaa ggcatgaccg tagcgaatac aggcagcgtc agccaatcct acgacgcaga
      481 tacgcgtgca tcgtatctgg tgattgacgg agagaacatc accattcgcc gggtcgagta
      541 cgacgtggag agtgaagcag aagaactctt gcgttcgggg ctaccccacg ccgactggct
      601 gtcccggatt ctcctcgccg gaaaatactg tcccccggag tagaaaaatc tgttccagtt
      661 cgacgatcat cgccagcaga ctggcacatt gaggaggcgc tccggcgccg tggcgcgcag
      721 tcataaagcg ttcaacagga ggatcacatg gccattgaaa tacgagggat ggcgccgctg
      781 ctgcaagtct tcgacatgcc cagatccatc gcgttttacc gggatgtgct gggcttcgag
      841 gtggtcagca cgtccacgcc tcggggagag cacttcgatt gggcgctgct caggctcaat
      901 ggagtggagt taatgctgaa cacggcttac gaagaggacg cgcggccgcc agttcctgat
      961 tccgctcgga ttgcggcgca cgacgatacg tgcctttacc ttggctgccc ggacgtggat
     1021 gcggcttacg cccacctgcg ggcgcgtggc gttgcagcaa aggagcctaa ggtcgcgccc
     1081 tatgggatga agcagttgta cgtcagcgac cccgacgggt ataacctgtg ctttcagtgg
     1141 ccagctaagt agggagttcc aaaaatacat gaaggaaggc cgaaagtccc gacgatagca
     1201 agatggccac gcctgtacca cgagcgggtt gcgcgccttt ttgagagagg cctatactgg
     1261 cggagattcg cagcagtagg tgagaatcgt gcgaattcgt gtttcttggc gatagaatag
     1321 ctgccaggag cgtgcgggag cattgccagg cttggaatcc gcatgagaga ggaagaagcg
     1381 cgagtggagg aagtaaacca gtttccggcc gtcgaacgcc gccgcaatcc tgcggtgccg
     1441 tccgaacagc gggaaaaggg agaggttgcc atcttccagg agttggggaa ggcgctgacc
     1501 tcctcgctcg aactcgatca agttctccgc acgatcatgg agaagatcga tgaatttctg
     1561 cgcccggaca actggtcgct gctgctgctg gatgagtcga agcaggaact gtattttgag
     1621 ctggcggtgg ggaaagcttc gcaggcgctg aaggacgtac ggatcaaaat ggggcagggc
     1681 attgcgggct gggtcgcgca gcatggcgag accgtggtgg tcccggatac cgcgaaggat
     1741 acgcgatttt tctcgaaggt ggacgagaag accaagacgg agacgcagtc gatcgtggcg
     1801 gtgccggtga aattccgtga cacttgcctg ggagtgatcg agctgatcaa ttgcatcggg
     1861 ccggagggat tcgatccccg ggacctgaag ctgctcgaag cgctttcgga cttcgcggcg
     1921 attgcgcttg agaacgcccg gcacgtgaag aaaattcacg agctgaccat cacggacgac
     1981 tgcacttcgc tctataacgc gcggcacatg ggctttattc tggagacgga gatctaccgc
     2041 tcgcagcgat acaactacga attctcgatc gttttcatcg acctggacca tttcaagcag
     2101 gtaaacgata cgcacgggca tctggtgggc tcgcggctgc tggcggagat cgggaatgcg
     2161 ctgaaggagc attgccggct gatcgattac gcgttccgtt atggcgggga cgagtttgta
     2221 atcctgctgc cgcagacttc gaaagagaac gcactgaacg tggcgcggcg tttacacaaa
     2281 ttggtacgcg agaggacctg gctggtgccg gaagagttga acatccggct gacgcccagc
     2341 gtgggtgtgg cgtcctaccc ggcggattcg cgaaccaagg aaggattgct gcacctcgcc
     2401 gatgaggcca tgtacctggt gaagaatacg aggcgcgaca gcgtggccgc ggcgaatatg
     2461 gggatcttgc cgaatccggt ggcgagcgcc gagtcggccg gttagtcctc gcgttcttct
     2521 ggccgaacat ttcatcttct atacactcca tcgaaagttt gcttaactga agacatgaag
     2581 cgaagcgaag cggcgaagta tgcgcgctgg tcggcggcgg cggcgttgct gctggcgagt
     2641 ctgacggctg gggtgtatct cgagcgcaaa tgggtcgctt atcgagaaaa gcaaaaggcg
     2701 ccaccgccgg ctccgcaaga tgtgacgcgc agttcaaacg ggcttacctt ttccaagatg
     2761 gcggggaacc aaaagatttt caccgtggag gcgtcgaagg ccacggattt caaggacaag
     2821 gccgccagtt tgctggaaga cgtgaagatc accatcttcg ggaagaccgg cgagcgtcac
     2881 gacacgattc atacgcaaag ctgccagtac gaaaaattgg gcggaagcat cgcatgcagc
     2941 ggcgaagtgc agctcgacct agagagccgg gcggaagccg agcgggcagc aaaaaatccg
     3001 gaaatagcag cgcagcagaa ggtacacgtg gagacccgag gggtggtctt taaccgcgca
     3061 agtggtttgg cgcggacgga acagcccgtg aagtttgttt ttccgaatgg gaatggggaa
     3121 gccgtgggag tcgagtatca ctccgaagaa gggacggtgc gattgctgag agacgtgcag
     3181 ttctctctga tgccgccgag aaacgacagc gcggggaaga agacggcgag tgcgggtgcg
     3241 aaagaaccca ttcgtgtgac ggggaagagc ctcgattttg agcgggactc gaggaccatg
     3301 cagttggacg ggccggtgga agcggaaact cacacggcgc gtttgcgcgc gggaaagttc
     3361 acc
//
LOCUS       DASYVO010000177         4785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_73605, whole genome shotgun sequence.
ACCESSION   DASYVO010000177 DASYVO010000000
VERSION     DASYVO010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73605"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            398..625
                     /locus_tag="VGR03_05985"
     CDS             398..625
                     /locus_tag="VGR03_05985"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1993860.1"
                     /translation="MVPAHYHTAEEQLIVVRGDVLTGMDGMPEKTLGPGGFAMMPGKV
                     MHWFTCQSKEACLMFVTFDRTYDIVWAKPQK"
     gene            779..3295
                     /locus_tag="VGR03_05990"
     CDS             779..3295
                     /locus_tag="VGR03_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEV1993861.1"
                     /translation="MTQPSKGAAVFLTLFGLPFLGAGLAFIYAQLVSRGNFSTFNTVA
                     AILFGSVFVLIGGGLIYAAIGGYGRLKKQAAIEESNPLSPWLWRTDWASRRAESQNKK
                     SEIFFWVLAIFCNLITLPFLFGMVPKFVRSGDLRVFLLLGFNLLGAILIVKAVRATIR
                     HRRFGNTYLEFDVLHFSPGERVGGRIHLQLDTRAEHGVDLRLSCVRKIVSESGNSRST
                     NQVILWQADQNVPAEAVGPGPLGRAIPVDFSIPAESPITNHDNPNDQVLWLLHAQADV
                     PGVDYSDDFEIPVFRGATSAETARDSESPAFSGANAFGFATTSSDDSGAVAQPAHAKV
                     VISVGSGGTEFYFPALRNSGRAVMLFLFTFFWSAVVYVLYRVHAPIFFFIVFGLSDLL
                     IIAGFLHVTFGSARISVRSEEIVSRSGIFGLGRMRRIQVSDVASIVPVVNMQQGNSSG
                     NALHAIRMRLKDGRKIRLADEIDSRQEARWVVSQIETLAGLKLDTHVEVDLPLGVTPQ
                     LPGQTSGQVFTQARRPTSAAASAGVFIVVMAGMIGFIGWRMFSFTSRTKNSRNAAAAP
                     VKPTARRVFSAPLTDTDVERVRALPAQAQAEELLERAIGHDTRALALFDRQVGSWTGH
                     IRKSDRMKQLERRSEFSKDLRVRYANADINLALEGWQKNAQAADLLIEQARADVHYRS
                     AAVYFLGMLAGRGVDYERIHGVLLNYARDDKDATVRQWAVEGLRFLGKDEVLDELFTS
                     FTEDPANGVRDRAGCNISDCGIFTRKQRLKIVPKLIDLALNQRTTPQMRNWAFLALQE
                     ITDENLSADALVWSRWYQEHGSEKMAEFERLDWWQVRGDE"
     gene            3404..4330
                     /gene="mak"
                     /locus_tag="VGR03_05995"
     CDS             3404..4330
                     /gene="mak"
                     /locus_tag="VGR03_05995"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF007108.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HEV1993862.1"
                     /translation="MPTTQEKQEHIRIGVDLGGTKIEFVALERDGTELHRHRIATPRF
                     DYEGTVRAVAEAAEGIEKKLRRSATVGMGIPGTISTKTGLVKNANSTWLNGKPFDKDL
                     SRALGREVRCANDANCLAVSEATDGAGAGKHVVFAVILGTGCGGGIAVAGRVHSGSNG
                     VAGEWGHNTLPWMRPEEFPGPACYCGRNGCLETWISGTGLEADYKRATSVALKGPEII
                     ARSGAGESAALAALERFEDRLTRGLAQVINLLDPDVIVMGGGASQIPRLYQNVPVRLK
                     EYVFGQEADTPVLPAKHGDASGVRGAAWLWPL"
     gene            4455..>4785
                     /locus_tag="VGR03_06000"
     CDS             4455..>4785
                     /locus_tag="VGR03_06000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1993863.1"
                     /translation="MAEVQSTGTQAAVRNISDTARWAAVFRARETERADALFRDPYAE
                     RLAGTLGVDIANTLPQGNSHAWAWVARTYLFDQFITQELQQGADMVVNLAAGLDARPY
                     RMSLPASL"
BASE COUNT          932 a         1368 c         1460 g         1025 t
ORIGIN      
        1 ctcggccgca acccccgcag gcatcgcctc accacgtcca tcgccccatc tttttcctgc
       61 tgtcatctgc gttttgggcc gtgttctcct cagtcggatg tgatagataa cagtgtgcga
      121 cattgttcgc cacgcgcgtc tggagcctga acatgagaaa aattagcatc agttctgcga
      181 aaaccatgaa gacgtttctt tgtatagcgc cattgctact tgttctcgct gctgttgctg
      241 cattgcgagg gcagactcct ccgcacggta tagtcacgcc gctcgccagt gccaagctcg
      301 ttttcgacgg cgaacccgag tgtctcaaag ttgcccgcga gaacggcgac ccagataagg
      361 gcccttccac gtttcttctc gaagctcctt ccggtgcgtg gttcccgcgc actatcacac
      421 cgcggaagag caattgatcg tggtccgcgg cgatgtcctc accggcatgg acggcatgcc
      481 tgaaaaaact ctcggccccg gcggctttgc catgatgccc ggcaaagtca tgcactggtt
      541 cacctgccaa tccaaggaag cctgcctcat gttcgtcacc ttcgaccgca cctacgacat
      601 cgtctgggcc aagccccaga aatagctgtc acagtgcagg cgggttgcgc gcctcgcgag
      661 tcttcatgcg ctgatcgcgc cggacaaccc ggcctgtccc taagttcagg tatgactgca
      721 aaggcaattc agcttgtaca ttccgctttg tcctcccgaa cgattctaga atcgaggaat
      781 gactcagcct tccaaaggcg ctgctgtttt cctgaccctc ttcggattgc cctttctggg
      841 agcgggtctc gccttcattt atgcgcagct cgtcagtcgc ggaaatttca gcaccttcaa
      901 cacggtcgcc gcgatcctgt tcggctcggt ttttgtcctc atcggcggag gtctcattta
      961 tgcggcgatc ggcggctacg gccggctgaa gaaacaggcc gcgatcgaag aatcgaatcc
     1021 gctttcccct tggctgtggc gaacagattg ggcatcgcgc cgcgcggaaa gtcaaaacaa
     1081 gaagagcgag atcttctttt gggtcctcgc cattttctgc aacttgataa cgctgccgtt
     1141 tctattcggc atggttccga agttcgtgcg cagtggcgac ctgcgcgtgt ttctgctcct
     1201 cggcttcaac ttgcttggcg cgattctgat cgtcaaggca gtgcgcgcca cgattcgcca
     1261 tcggcggttt ggcaacacgt acttggagtt cgatgtactg catttttctc ccggcgaacg
     1321 cgtgggagga aggattcatc tgcagctcga tacacgcgcg gaacatggcg tcgacttgcg
     1381 gctctcctgt gtccgcaaaa tcgtttcaga gtcgggcaac agccgcagta cgaatcaggt
     1441 gatcctgtgg caggcggacc agaatgttcc cgcggaggcg gtggggcctg gaccgctcgg
     1501 ccgagccatc cctgtggatt tttcgattcc cgcggagagt ccgatcacga atcacgacaa
     1561 tccaaacgat caagttctgt ggctgctcca cgcgcaggcg gacgtgccgg gagtggatta
     1621 ctcggacgat ttcgaaattc cggtttttcg gggcgcaacg tctgccgaga cggcccgcga
     1681 ctctgagtcg cccgctttct ccggcgcgaa tgctttcggt ttcgcgacaa cgagcagcga
     1741 tgattccggc gcggtcgcgc agcccgcgca cgcgaaagta gtcatttcgg tgggctccgg
     1801 cggcaccgaa ttttatttcc cggctctgcg caattccggt cgcgccgtga tgctttttct
     1861 cttcacgttt ttttggagcg cggtggttta cgtgctgtac cgtgtgcacg caccgatttt
     1921 ctttttcatt gtttttgggt tgagcgattt gctcataatc gccggctttc tgcatgtcac
     1981 gttcggcagc gcgcgcatca gcgtccgcag cgaagaaatt gtttcgcgct cgggaatttt
     2041 cggccttggc cgcatgcgcc ggattcaagt ttccgacgtc gcctcgatcg tgcccgtggt
     2101 caacatgcag cagggcaaca gttcggggaa tgcgctccac gcgattcgaa tgcgtttgaa
     2161 ggatgggcgc aaaatcaggc tggcggatga aatcgacagc cgccaggaag cgcgctgggt
     2221 ggtctcgcag attgaaacgc tggcggggct gaagttggat acgcatgtgg aagtggattt
     2281 gcctctcggg gtgactccac aacttcccgg gcaaacatca ggccaagttt tcactcaggc
     2341 gcggcgaccc acttccgcgg cagcatctgc aggcgtgttt atcgtcgtga tggccgggat
     2401 gattggcttt atcgggtggc ggatgttctc gttcacttcc cgcacgaaaa actcgcgtaa
     2461 cgcagcggcg gcgcctgtta agccgactgc gcggcgcgtc ttttccgcgc ccttgacgga
     2521 cacagatgta gagcgcgtgc gcgcacttcc tgcgcaagcg caggctgagg aattgctgga
     2581 acgcgccatt ggtcacgata cgcgcgcgct ggcgcttttc gatcggcaag tcggaagctg
     2641 gaccggccac attcgaaaga gcgatcgcat gaagcagcta gagcgacgct cagagttttc
     2701 caaggatctt cgcgtgcggt acgccaatgc ggacatcaat cttgcgctgg aaggctggca
     2761 aaagaatgcg caggcagctg atttgttgat cgagcaggcg cgcgccgacg tgcattaccg
     2821 ttcggcggca gtctactttc tcggtatgct cgctggccgc ggtgtggact acgagcgcat
     2881 ccacggcgtg ctcttgaact atgcccggga cgataaggac gccacggtgc ggcagtgggc
     2941 cgtcgagggg ctgcgatttc tcggcaagga tgaagtgctt gatgagctgt tcacttcgtt
     3001 cacggaagat cccgccaatg gcgtgcggga tcgcgcgggc tgcaatattt ccgattgcgg
     3061 aatattcacg cgcaagcagc gcttgaagat agtgccgaaa ctgatcgatc tggcgctgaa
     3121 ccagcggaca acgccgcaaa tgcggaactg ggcttttctc gcactgcagg aaatcacgga
     3181 tgaaaatctg tccgcggacg cgctcgtctg gagccgctgg tatcaggagc acggctccga
     3241 aaaaatggct gaattcgaaa gactggattg gtggcaggtc cgcggcgacg aataacgccg
     3301 gagctctcaa ggtctcctcg atcgaaagaa gttcaacgac acacccattt acagcggggc
     3361 ggcgcggtgt agactacctc ctcgccggag gtatgcttca tcgatgccga ccactcagga
     3421 aaaacaagag cacattcgca ttggcgtgga tctcggcggc accaagattg aattcgtggc
     3481 gctcgagcga gacggcacgg aattacaccg ccaccgcatc gccacgccgc gcttcgacta
     3541 tgaaggcacg gtgcgcgccg tcgcagaagc tgcggaagga attgagaaaa aactccggcg
     3601 cagcgctacc gtgggcatgg gaatccccgg aaccatttcc acaaagacgg gtttggtgaa
     3661 gaacgcaaac tcgacgtggc tgaacgggaa accgtttgac aaggacctga gccgcgcgct
     3721 ggggcgcgaa gtacgatgcg cgaatgacgc caactgcctg gcagtttcag aagcaaccga
     3781 tggcgcgggt gcgggcaagc acgttgtttt cgcggtcata ttgggcacgg gttgcggcgg
     3841 aggaattgcg gtcgctggcc gtgtacacag cggttcgaac ggggtcgcgg gcgaatgggg
     3901 gcacaacaca ttgccgtgga tgcggcccga ggaatttccc ggtcccgcgt gctactgcgg
     3961 gaggaacggc tgcctggaaa cctggatttc cggaacaggc ctggaagcgg attataagcg
     4021 cgcaacgagc gtcgctctga agggccccga gattatcgcg cgaagcggcg cgggcgaatc
     4081 tgccgcgctt gccgcgttgg agcgattcga agaccggctc acgcgcggtt tggcacaagt
     4141 catcaatctt ctcgatccgg atgtgatcgt gatgggcgga ggcgcttcgc aaattccacg
     4201 gctctaccaa aatgttccgg tacgcttgaa ggagtacgtt ttcggtcaag aagcagacac
     4261 gccggttctt ccggcgaaac acggagatgc cagcggcgtg cgtggcgcgg cctggctgtg
     4321 gccgttgtag gtggatcgag cggcgccgct gaacatttag atttcccaat acacaacgtc
     4381 ctattttttg ccagacttgg gatcatcttg cgggcgggga cacactggcg gttcctgcca
     4441 aaaggaccag aactatggcc gaggttcaat cgacaggtac gcaagccgcc gttcgaaaca
     4501 tttcggatac ggcgcgatgg gcggcggttt ttcgcgcgcg tgaaacggag cgtgctgacg
     4561 cgctgtttcg cgatccgtac gcggaacgac tcgcgggcac gctcggcgtg gatatcgcca
     4621 acacactgcc gcagggaaac agtcacgcgt gggcgtgggt cgcccggact taccttttcg
     4681 atcagttcat cacgcaggag ctgcagcagg gcgcggacat ggtcgtgaat cttgccgcgg
     4741 gcctcgacgc gcggccgtac cgcatgtctc tgccagcttc gctcc
//
LOCUS       DASYVO010000178         5034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_48416, whole genome shotgun sequence.
ACCESSION   DASYVO010000178 DASYVO010000000
VERSION     DASYVO010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48416"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VGR03_06005"
     CDS             <1..791
                     /locus_tag="VGR03_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV1993864.1"
                     /translation="AFTGSRDVGLDIHLGAAQTQPGQIWIKRTVLEMGGKDAIVVDEE
                     ADIDAAVEGTAQAAFGYQGQKCSACSRVIVSEKIYDTFVQKLVERTKKITVGPSDDPN
                     NYMGPVISQSAMKTILDYIEVGKKEGKLLTGGKRAAGDGYFIEPTIIGDVAPKARLAQ
                     EEVFGPVLAVIKAKNYDQALEIANNTEFGLTGAVYSKNPEKIKKAEETFHVGNLYLNR
                     KCTGAMVGAHPFGGFNMSGTDSKAGGKDYLLLFMQGKSIAEKVS"
     gene            complement(788..2218)
                     /locus_tag="VGR03_06010"
     CDS             complement(788..2218)
                     /locus_tag="VGR03_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV1993865.1"
                     /translation="MSGTSEASSTVSSPPAVGRGRLLQVLGMWFGMAAAIGNTIAAGI
                     VRTPGEVAKLLPNAWMFLGVWVLGGLYAFSGASSMAELGAAIPRSGGQYNFSRRALGD
                     YAGFIVGWSDWLSTCGTAAAVAIVIAEYSGALFPVLSGNVTILGFATRIPLIVAVLVI
                     VGFFVLQWRGIRWGSGAQLSTAALKTSAFVILVIACFLLGGPAHRAAQQSAASALALP
                     SGWPLLVAIMLGLQGVIYTVDGWDGVIYFGEEVRNPGRDIPRAIFGSVFSIMGIYLLL
                     NLVALYILPMNKISGNDFVLGTVADLIFGKLGDPIIRSIMVISMLSCLNANQLFCSRT
                     LYTMSCDGLFFRRAARVNPGGTPVLALFLSTVVGVLFLLTGTFERVIAMLSFFFVANY
                     ALSYASLFVLRRREPQMARPYRAWGYPWTTGIALGASALFLIGSIVTDQKNAPWALAI
                     LVLSYPVFRVLKWVTQRADASAAANS"
     gene            complement(2321..3025)
                     /locus_tag="VGR03_06015"
     CDS             complement(2321..3025)
                     /locus_tag="VGR03_06015"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1993866.1"
                     /translation="MPTLPAETPENETNPQVQDAPSPDPWEAAYLRFETPEEEIQKFI
                     VRLNRLGAPQWPRDAEIVELFCGRGNGLVALDRLGFTRIEGVDLSPRLVAQYRGPAKC
                     IVGDCRQLPFADRSKDVLIVQGGLHHLPTLPDDLQQTFAQMQRVLRKDGRAVFVEPWL
                     APFLKFVHWVSENPLARRVSNRMDALATMIQFERRTYEQWLGQPELIKKIARAHFVPV
                     HESFAWGKWNFVGTPR"
     gene            complement(3160..4887)
                     /locus_tag="VGR03_06020"
     CDS             complement(3160..4887)
                     /locus_tag="VGR03_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV1993867.1"
                     /translation="MVTFVDVTQRRQAEQVLRNAKEGAEAANEAKSIFLATMSHEIRT
                     PMNGILGMTELVLDTDLTVEQRENLGLVRLSAESLLSIINDILDFSKIEAGKLEIEAI
                     PFDLRESLGETMRSLSVRAHQKGLELIYDVQPDVPEALIGDPGRIRQVLVNLVGNAIK
                     FTEQGEVFINVEEESQEDAITCLHFTVKDTGVGIPSEKQSKIFEAFLQADGSMARRYG
                     GTGLGLTICTRLVTMMGGAIWVESQPNQGSTFHFTLRSALQDTPRPRSEPLHSEQLRD
                     LHALIVDDNFTNRRVLNGMLTRWGMKPTAVEGGRAALQALEVAKSTGRPFPLILLDGQ
                     MPEMDGFTLAERIKKDPELVGATIMMLTSAGHLGDAARCRELGISAYLVKPIRQGELL
                     QAICNVLNLAPQKQAPLITRHTLREARNRSRVLLVEDNAVNQLLALRLLEKRGYIVSV
                     AADGRKALAALEKEDVDVVLMDIQMPEMDGFEATAAIRERERSTGRHIPIIAMTAHAL
                     KGDEERCLSAGMDAYISKPIRTNELFATIERVLGKTGEPTPSTAVGNPKLPAHRNQRL
                     LQPEAKTKS"
BASE COUNT         1149 a         1371 c         1522 g          992 t
ORIGIN      
        1 tcgctttcac gggctcgcgc gatgtcggtc tggacataca tcttggcgcg gcccagacgc
       61 agcctggcca gatctggatc aagcgcacgg tgctggagat gggcggcaaa gacgcgatcg
      121 tggtggacga agaggccgac atcgacgcgg cggtggaagg cacggcgcag gcggcgttcg
      181 gctatcaggg acagaagtgt tcggcatgct cgcgcgtgat cgtctccgag aagatttacg
      241 atacgtttgt gcagaagctg gtggagcgca cgaagaaaat taccgtcgga ccgagcgatg
      301 atccgaacaa ttacatgggg ccggtgatca gccaatcggc gatgaaaacg attttggatt
      361 acatcgaagt cggaaagaag gaagggaagc tgctgaccgg cgggaagcgc gcggcaggcg
      421 acggctactt catcgagccg acgattattg gggatgtcgc tccgaaggcg cggctggcgc
      481 aggaggaagt gtttggcccc gtgctggcgg tgatcaaggc aaagaattac gaccaggcgc
      541 tggaaattgc gaacaatacg gaatttggct tgacgggagc ggtgtactcg aagaatccag
      601 aaaaaatcaa aaaggctgaa gagacgtttc acgtaggcaa tctttatcta aatcggaagt
      661 gcaccggcgc gatggtcggc gcgcatccgt ttggcggatt taatatgtcg ggaacggact
      721 caaaggcagg cggaaaagat tacttgttgc tgttcatgca ggggaagtcg atcgcggaaa
      781 aagtgagcta agagtttgct gccgcgctgg cgtcggcgcg ctgcgtgacc cacttcaaaa
      841 cgcgaaaaac gggatagctc agcacgagga tcgcgagcgc ccacggggcg ttcttctggt
      901 cggtgacgat cgacccaatc aagaaaagcg cagaggctcc cagcgcaatt ccggttgtcc
      961 acgggtaacc ccacgcccgg tacggccgcg ccatctgagg ctctctccgg cgcaacacaa
     1021 acagcgaggc gtacgacagg gcataattgg caacaaagaa aaaagagagc atggcgataa
     1081 cgcgctcgaa ggttcccgtc agcaggaaga gcaccccgac aacggtgctg aggaagagcg
     1141 cgagcacagg cgtgccaccc ggattgacgc gagcggcgcg gcgaaaaaat aatccgtcgc
     1201 agctcatggt gtaaagcgtg cgcgagcaga aaagctggtt ggcgttgagg cagctcaaca
     1261 tggagataac catgatggag cgaatgatgg gatcgccaag cttgccgaaa atgagatccg
     1321 cgacggtgcc gagaacaaaa tcgtttccgg agattttgtt catgggcagg atgtacagcg
     1381 ccaccagatt cagcaacaaa taaattccca tgatggagaa gacgctgccg aaaatggcgc
     1441 gcgggatgtc gcggccggga ttgcgcactt cttctccgaa gtagatgacg ccgtcccatc
     1501 cgtcgacggt gtagatcacg ccctgcagcc cgagcatgat ggcaacgaga agaggccatc
     1561 cggaaggcaa tgcgagtgcg gaagcggcgg actgctgggc ggcacggtga gccggcccgc
     1621 ccagcaaaaa gcaggcaatc acgagaatca cgaatgcgct ggtcttgagc gcggcggtcg
     1681 ataattgcgc gccgctgccc cagcgaatcc cgcgccattg cagaacgaaa aatccaacga
     1741 tcacgaggac ggccacgatt aatggaatgc gcgtggcgaa accaaggatc gtcacgtttc
     1801 cgctcaggac agggaaaagc gcgccactat attcggcgat gacgatggcg acggcggcgg
     1861 cggtgccgca ggtggagagc cagtcgctcc agccgacgat gaaacctgcg taatcgccga
     1921 gcgcgcggcg cgagaaattg tactggccgc cgctgcgggg aatggccgcg cctagctccg
     1981 ccatggaaga ggcgccggaa aatgcataga gtccgccgag gacccaaact ccgagaaaca
     2041 tccaggcatt ggggagaagc ttggcgactt cgccgggcgt tcgaacgatg ccggcggcga
     2101 tggtgttgcc gatcgcggcg gccatgccaa accacatgcc gaggacctgg agcaggcggc
     2161 cgcggccgac cgcgggcggg gagctaactg tggaagaggc ttccgatgtg ccagacaatg
     2221 tgaggtctcg aggatgcgcg aaacgcagcc atcatatcgg ggggatgaga aaatgcaagc
     2281 tggaagtggc gactttgaag gctattcgtt cggcaaagtc ttatcgcgga gtgccgacga
     2341 aattccattt gccccaggcg aaagactcat gcaccggcac gaagtgagcg cgggcaattt
     2401 tcttgatgag ttcgggctgg cccagccatt gctcataggt gcggcgttcg aattgaatca
     2461 tggtggccag tgcatccatc ctgttcgaga cgcggcgtgc aagggggttt tcggaaaccc
     2521 agtgcacaaa tttaagaaat ggagcgagcc acggttccac aaacaccgcg cggccgtctt
     2581 tccgcagcac gcgctgcatc tgcgcaaaag tttgttgcag atcatccggc agagtcggca
     2641 aatgatgcaa accgccctgc acgatcagca catccttgct gcgatccgcg aaaggaagtt
     2701 gacggcagtc gccgacaata catttcgccg gcccgcgata ctgggcgacc agccgaggcg
     2761 aaaggtccac gccctcgatc cgggtgaaac cgagacgatc gagagcgacc aaaccgtttc
     2821 cgcgcccgca aaaaagctcg acaatttcgg cgtcgcgcgg ccattgagga gcgcctaacc
     2881 gattgaggcg cacgataaac ttttgaattt cctcttccgg cgtttcgaaa cgcaagtacg
     2941 cggcttccca gggatccggg gagggggcgt cttgcacctg gggatttgtt tcgttttccg
     3001 gcgtttcagc tggtaatgtt ggcatcttcg gtggacggaa gtgagaacca tcgaaggttc
     3061 ccatagatcg ccgatcaatt caagacaggg gtcaaggcat gggtcaagac acgatgccct
     3121 tggttagcga gtgagtattc tgtaagccct gtacccaccc taggactttg tcttggcttc
     3181 gggttgcaag agccgttgat tccggtgtgc gggtagcttt gggtttccga cagcagtgga
     3241 aggggtgggc tcacccgtct tgccaagcac gcgctcgatg gtcgcaaaca gctcgttcgt
     3301 ccgaatcggt tttgaaatgt aagcgtccat gccagctgac aggcagcgct cctcatcacc
     3361 cttgagggca tgtgcggtca tggcaatgat tgggatgtgc ctgccggtag accgttccct
     3421 ttcccgaatg gcggcagtgg cctcgaaacc gtccatctcc ggcatttgga tgtccatcag
     3481 cacgacatca acgtcctcct tctcaagcgc agccagtgct ttccgaccgt ccgcggcgac
     3541 tgaaacgatg taccctcgtt tttcaagaag acggagtgcg agcagttgat ttacggcgtt
     3601 gtcttccacc aacaggactc gcgaccggtt cctagcctcc cgcaacgtat gacgggtgat
     3661 taatggagct tgcttctgag gtgccagatt caagacatta cagatcgctt gcagtagctc
     3721 tccctgacgt atcggtttca cgaggtaggc ggaaattccg agttcccgac aacgagctgc
     3781 atcccccaag tgcccggccg acgtaagcat cataatggtg gcaccgacca gctccggatc
     3841 tttctttatc ctttcagcca gggtaaagcc gtccatctcg ggcatctgtc catccaacaa
     3901 gataagagga aagggacgac cggtgctctt ggcgacttcg agcgcttgaa gcgctgctcg
     3961 gccaccctcg acggccgtcg gcttcatgcc ccaacgagtt agcatgccgt tcagaactct
     4021 tcggttggtg aagttgtcgt ccacaatcag ggcgtgtaga tcgcgcaatt gttccgaatg
     4081 gagaggttcg gaacgggggc ggggagtatc ctgcaaagcc gaccggagcg tgaagtgaaa
     4141 agtgctgcct tggttaggct ggctctccac ccatatggct ccacccatca tggtcacaag
     4201 tctcgtgcag atcgtcagtc ctaacccagt gccgccgtat cttcgggcca tcgagccatc
     4261 ggcctgcaaa aatgcttcaa aaatcttcga ttgcttttcc gatgggatgc cgacgcccgt
     4321 gtcctttaca gtgaaatgca agcaagtgat ggcgtcttct tgggattctt cctccacatt
     4381 aataaagacc tcaccctgtt cggtgaactt gattgcgttt ccaaccaagt tgacgagaac
     4441 ttggcgaatc cgcccgggat caccaatcaa cgcttcgggg acgtccggtt gaacatcgta
     4501 gattagctcc agcccctttt gatgtgcgcg aacactaagc gacctcatcg tctcgccaag
     4561 gctctcgcgc agatcaaatg gaattgcttc gatctcgagc tttcctgctt caatcttcga
     4621 aaagtccagt atgtcgttga ttatggagag cagtgattca gccgagagtc gcacgaggcc
     4681 gagattctcg cgctgctcga cagtaaggtc ggtgtccagg accaattcag tcattccaag
     4741 gatgccgttc atgggcgtgc gaatttcgtg gctcatggtc gccagaaaaa tgcttttcgc
     4801 ctcgttcgca gcttcggcgc cttctttggc atttcgtaag acctgctccg cctgcctgcg
     4861 ctgcgtaacg tccacaaacg tcaccaccgt gccgattagc tcctcgcccc ggtgcaatgg
     4921 ccgggaccaa tattccgccg ggaagctggt gccatctttt cgccacaaga cctcgtcatc
     4981 cacgtgtgtg ccgtgacccg tccggaaagc ttcgtagatg tggctttgct gcac
//
LOCUS       DASYVO010000179         6136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_214438, whole genome shotgun sequence.
ACCESSION   DASYVO010000179 DASYVO010000000
VERSION     DASYVO010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214438"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VGR03_06025"
     CDS             complement(<1..533)
                     /locus_tag="VGR03_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEV1993868.1"
                     /translation="MNSLFTRWTDWTVASGTRLAGILLVSILLVRLVRSLSRRMVKEA
                     TTPTRAAQMREQQMRTLAGILASIGATVIIVVAVLTALPEFGVNVTPIEAVAGLASFA
                     VGFGAQNLVRDWISGVLIVVEDQFVIGDLVRIGDTLGRVESLTLRRTVLRDLQGALVH
                     ISNGEIRQAANLSRDWS"
     gene            627..989
                     /locus_tag="VGR03_06030"
     CDS             627..989
                     /locus_tag="VGR03_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993869.1"
                     /translation="MPSSRRTFLSLGLPLSLVAAAAAAAQRRPRFHVDQPGSGRRMNS
                     ADWNLGADQPRPDPRVALRERHEEIKKQVRQLYDLAGELKMEAEKTDSAAVLSLPVLK
                     KAEQIQKIAKHIQSLARG"
     gene            1148..2227
                     /locus_tag="VGR03_06035"
     CDS             1148..2227
                     /locus_tag="VGR03_06035"
                     /inference="COORDINATES: protein motif:HMM:NF014827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEV1993870.1"
                     /translation="MNLLKAMTAHDYEQVIAFQNRATGLRGFLTLHDTTLGPALGGVR
                     IRTYTSEQEALTDSLRLSRAMTYKAAISGLPCGGGKTVILLDADLKREAAFEALGRMI
                     ESLHGRYFCARDVGITDADLGAIARATRFVAREPSPELGDVSEHTARGVWQGMRACLE
                     RAGIKGPARVAIQGVGSVGRELARILRKQGMDLVVADVDAARAAEVAGETGARVVDAQ
                     EILYAPCDVLAPCALGGVINAESVPRLQCRIVCGSANNILATLEDGDALDRRGVLYGP
                     DYLVNAGGLIRGAEFFLLGRADSSASIAALYERTRGVLELARERGISTARAADQVAEA
                     RLKKAKSFRDLTWEATGALSSPARV"
     gene            2255..2860
                     /locus_tag="VGR03_06040"
     CDS             2255..2860
                     /locus_tag="VGR03_06040"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV1993871.1"
                     /translation="MRVELVTLEGEHVRLEPLTLEHLDALCDIGLDEDLWPCIPVPVR
                     TREEMRAYIEKALGERDAGMALPFATIHRESGRAIGCTRYGRIDRTHRRVEIGWTWIG
                     RDWQRTAVNTEAKYLMLRHAFERLGCIRVELKTDSLNERSRNAIQRLGAKEEGIFRNH
                     MITASGRLRHTVYYSILDAEWPAVKSRLEEKLSRPSAIPSA"
     gene            2986..3062
                     /locus_tag="VGR03_06045"
     tRNA            2986..3062
                     /locus_tag="VGR03_06045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3020..3022,aa:Arg,seq:ccg)
     gene            complement(3077..4237)
                     /locus_tag="VGR03_06050"
     CDS             complement(3077..4237)
                     /locus_tag="VGR03_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993872.1"
                     /translation="MSRHQEGYIWRKGHSWYGRWWDDVIEDGRIVRKQRSRRLAIYCD
                     RYRSEKDVRPLLEDILRPINEGRASPQSTLMVAEYVEIHYLPYAQENYKPSTYAGYKT
                     LWNLYLVPRLRNVALRDFRTVDAAILLADLHRAHALGRNTLKHIKSLLSGVFTYAKNQ
                     GVLDGLNPIRDAFVPKKSAGPRETFATSPDDVLKIIDILATAGHLKASAAVALIFFAG
                     LRPGEARGAGWDDYDGKCIVVRHAVWRTFTTSPKTPESSQPVPVIEPLRSILTELRKV
                     DGDPESGPILRGPSSKPLNLDNLSRRVVIPTLKAAGLEWHGWYSLRRGIGTLVHTIEK
                     NAMAAKGLLRHCSVTTTQRHYIKDVPEITLNAMKKVEALCNDRATALTAKPS"
     gene            complement(5276..5947)
                     /locus_tag="VGR03_06055"
     CDS             complement(5276..5947)
                     /locus_tag="VGR03_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993873.1"
                     /translation="MSRDLMTGEQAGLQLKKVKSKPKTTQLYAKRCLFPLSAGEDLRI
                     RKTLLETLAIDKSLLEMTNPGVPRSRLRLFVQGVEQAWLVSRYKIQNRQPNLILWLRG
                     LLKECHQAGLWIPSICIRRLRQLERGELGVRDRHGRLELPRFEGLPSVFVEACEFMGW
                     GPMGIVPRYDGTYGNAWKVDKDEIEFVQIVCHKWKGRPRTMKRAELCAWFIAEVGEAG
                     WTVNH"
BASE COUNT         1177 a         1845 c         1841 g         1273 t
ORIGIN      
        1 tgcgaccagt cgcggctcaa atttgccgcc tggcggatct cgccgttcga gatgtgaacc
       61 agcgcgccct gcaggtcgcg aagcacggta cggcggagcg tgaggctttc cacgcggccc
      121 agcgtgtcgc cgatgcgcac caggtcgccg atgacaaact ggtcttcgac cacgatgagg
      181 acgccgctga tccaatcacg caccaagttc tgcgcgccga atcccaccgc gaagctggcc
      241 aggcccgcca ccgcctcgat gggcgtgacg ttgacgccga attcgggcag cgccgtgagc
      301 accgcgacga cgatgatgac cgtggcgccg atgctggcga ggatgccggc gagcgtccgc
      361 atctgctgct cgcgcatctg ggccgcacgc gtaggcgtag tggcttcttt caccatccgg
      421 cgggagagcg agcgcaccag gcgaaccaga agaatagaaa ccagcagaat tccggcgagg
      481 cgcgtgccgc tcgctacggt ccagtccgtc caacgggtga aaagcgaatt catcgagatg
      541 gccgttcccg tgaagccgaa agagtatatc attggcagga gaacataccg ccgcgtccgg
      601 gcatcttggg ccggaggagg ccagccgtgc catcttctcg tcgtactttt ctttcgcttg
      661 gattgccgct gtcgcttgtg gccgctgcgg ccgccgccgc tcagcgccgg ccgcgctttc
      721 atgtcgatca acccggttcg gggcgaagga tgaattcggc tgattggaac ctgggagccg
      781 atcagccaag accggatccc cgggtcgccc tcagagaaag acacgaggag atcaagaaac
      841 aggtgcgtca actttacgat ctcgccggcg agttgaagat ggaagccgag aaaacagatt
      901 ccgccgcggt gctctccctg cccgtgctga aaaaagcgga acagattcag aaaatcgcca
      961 agcacataca gtcgctggct cgcggctgaa gcggctgcgc gcaatccctg cggccatttt
     1021 ggccgttgtc cctcccgccc ggaatcgtta ccgtaacatt ttccattcct cacagaagga
     1081 cgccgtgctg gagctgttct gagctctgcg cggtcaaacg aacgcaggaa taagtcacgg
     1141 aggggcgatg aatctcctca aggcgatgac cgcgcacgat tatgaacagg tgatcgcgtt
     1201 ccagaaccgc gccaccggcc tgcgcggatt tctgacgcta cacgatacca cgctgggacc
     1261 ggcgctcggc ggcgtgcgca ttcgcaccta caccagcgaa caggaggcgc tcacggactc
     1321 gctacgcctg tcgcgcgcca tgacctacaa agcggcgatc tccgggctgc cgtgcggcgg
     1381 gggcaagacg gtgattctgc tcgacgccga cctgaagcgc gaggccgcgt ttgaagcgct
     1441 cgggcggatg atcgaaagcc tgcatgggcg atacttctgc gcgcgtgacg tggggatcac
     1501 ggatgcagac ctgggcgcga ttgcgcgcgc gacccgattc gtggcccgcg aaccgtcgcc
     1561 ggagttgggc gacgtcagcg agcacacggc gcgcggcgta tggcagggga tgcgcgcgtg
     1621 cctcgagcgc gccggaatca aggggccagc gcgcgtggcc atccagggtg tgggcagcgt
     1681 cggtcgtgag ctggcgcgga ttctgcgcaa acagggtatg gatctggtgg ttgccgacgt
     1741 ggacgcggcg cgcgcggcgg aggtcgccgg cgaaaccgga gcgcgcgtgg tggacgcgca
     1801 ggaaatcttg tacgcgccgt gcgacgtgct ggcgccgtgc gcgttgggcg gcgtgatcaa
     1861 cgcggagagc gtgccgcggc tccagtgcag gatcgtctgc gggtcggcga ataatattct
     1921 tgccacgctc gaagatggtg acgcgctcga ccgccgcgga gtgctctatg ggccggatta
     1981 tctggtgaat gccggcgggc tgatccgcgg cgcggagttt ttcctgctgg gccgcgccga
     2041 ctcgagtgcc agcatcgcgg cgctctatga gcgcacgcgg ggggtgttgg agctggcgcg
     2101 cgaacgcggc atatcgaccg cgcgcgccgc cgatcaggtc gccgaggcgc gattgaagaa
     2161 agcgaagtcg tttcgcgacc tcacttggga agccacgggc gctctttcct ctccggcgcg
     2221 agtgtgagcg atggggacgc cgatgcagga accgatgcgc gttgaactgg taacactcga
     2281 gggcgaacac gtgcggctcg aaccgctgac cctcgaacat ctcgacgcgc tgtgcgacat
     2341 cggcctcgac gaagacttgt ggccttgcat tcccgtgccg gtgcgcacgc gggaagaaat
     2401 gcgcgcctac atcgaaaagg cgctagggga aagggacgcg ggcatggcgc tgccgttcgc
     2461 aacgattcac cgcgagtccg gacgagcgat cggctgtact cgctacggac gcattgatcg
     2521 gacccatcgc cgcgtggaga tcggctggac gtggatcggc cgggactggc aacgcacggc
     2581 ggttaacacg gaagcaaagt atctgatgct gcgccatgcg ttcgagcggc tgggatgcat
     2641 tcgcgtggag ctgaagacgg attcgctcaa cgagcgctcg cgcaacgcca tccagcggct
     2701 gggtgcaaaa gaggaaggga tttttcgcaa ccacatgatc accgcaagcg gccggctgcg
     2761 gcatacggtc tactacagca ttctcgatgc ggaatggccg gcggtgaagt cgcgattgga
     2821 agaaaaactt tcgcggccat ctgcaattcc ttccgcgtga accggctctg gaacgggccg
     2881 gggcgattct ctctcacgaa agcgcggcgt agagccgctt ctgcggcgac gggctggctt
     2941 gaccttcctc gggggacatc ctagactgaa atcagttcca tttgcgcgcc tgtagctcag
     3001 ttggatagag cgctggtctc cggaaccaga ggtcggaggt tcaaatcctc tcaggcgcgc
     3061 cacttctttc aatgacttaa ctgggttttg ccgtcagggc ggttgcacgg tcgttgcaca
     3121 acgcttccac ctttttcatg gcgttcagtg tgatttcagg cacgtctttg atgtagtgcc
     3181 tctgcgttgt agtaacgctg caatgacgca gcagaccttt cgcagccatc gcattcttct
     3241 cgatagtgtg gacgagcgtt ccgatgccac gccgaagtga gtaccatcca tgccactcca
     3301 gacctgcggc tttgagcgta ggaatcacca cgcgcctaga gagattgtcg aggttcagcg
     3361 gcttactcga agggccacgc aggattggac cggactccgg gtcgccgtcc actttccgca
     3421 gctcggtcaa gatggaacgc aatggctcaa taactggaac aggttggctg ctttctggag
     3481 tcttcggcga ggtcgtaaaa gttcgccaga ccgcatgcct taccacgatg cattttccat
     3541 catagtcgtc ccaccctgcc ccgcgtgctt cccccggacg caagccggca aagaagatca
     3601 aggcgacggc cgcgctggcc tttaggtgcc cagccgtggc gaggatgtcg ataatcttga
     3661 gaacgtcatc cgggcttgtc gcgaacgttt ccctgggccc cgccgacttc ttaggtacaa
     3721 aggcatcgcg gatcggattg aggccatcga gtacgccctg attcttggcg taggtgaaca
     3781 cgccactcag cagcgattta atatgcttca aggtgttgcg ccccaaagcg tgagcgcggt
     3841 gaaggtcggc aagaaggatt gccgcgtcca cggtccggaa atcgcgcaag gccacgtttc
     3901 ttagccgcgg aactaggtag agattccaca gagtcttgta accggcatag gtcgatggct
     3961 tgtagttctc ctgagcgtat ggcagatagt ggatctcgac gtactcggct accatcaagg
     4021 tactttgtgg gcttgctctg ccttcgttta tgggccgcag gatgtcttca agaagcggcc
     4081 gcacgtcttt ttctgaccgg tatcggtcgc aatagatagc cagcctgcgt gatcgctgct
     4141 ttcgaactat gcggccatct tcgatgacat cgtcccacca ccgtccatac caactatgcc
     4201 ctttccgcca aatatagccc tcttggtgtc ggctcattcc tttctcctct tgctgagcct
     4261 tcggccacgc tccaggcccc cgagtctcat caactctgac ggcggttcac cgcttgcttg
     4321 ggccagcttc tgaagttctc gccactcggt tctggttaac ctaaccggat aaacgaggga
     4381 ccgaacttga gaggaaggct tagggggtct gcccagtcgc tttgcggcca ttaggttcat
     4441 tctccttttt cgtataacgc aaatgtgtgg aactgtcaag acttttcgta tagcgattag
     4501 tgtttcttct ttccagccaa agaattacgt ccggccagta gaagcgtcgg tattttccca
     4561 tgcgaataaa cggtagggga tcggcattat ggaggtggcc tcgattgcgg gtttgctcgt
     4621 aaacccaact cctcggcact tgaagcctct ctgccaattg ttgcggcgtc aggaggtgaa
     4681 agggctcgat tgccggccgc tgttcctccc ctccgatggc ctcggattgt ctcaactgat
     4741 caccatttgc gcaaaagccg tgcaacgccc tttgtatctc attctaaaaa ccgctcttag
     4801 agctgactgc gggttacgct aaccggtgtt catggtctgt gggcctcgtc catgccgcgc
     4861 tcaactcaat cctgccccta aggcgctgtg tgaagcttca cagggtcctc agacgctccg
     4921 cttagcgaag acccccaccc gtcctccaat cgtggctcta cggtactgtc cgaagcttca
     4981 cggcgccctt ttaccctccg gttgtcactg acaacggttt gccgaccaag agcggcccgg
     5041 caagcagggc aacggagtcg cgaaaatatc ttttcaaatc tctcctgctc aagctgtctc
     5101 tgcagcctgg tcctgcgggc tttatcgatc cggagacagc gggcatggaa aaacttgcgc
     5161 gagccggcgg gtaatggttg gccgcagccg acgcagaacc gcactacttt ccctttctcg
     5221 gctgtcccat ccgtccgctc aacatccaaa tggtttacga gctctcgcgg agatttcaat
     5281 gattcaccgt ccagccggcc tcgccaactt ctgcgataaa ccatgcacaa agctctgccc
     5341 ttttcatcgt gcgtggcctt cctttccact tgtggcagac aatctggacg aactcgattt
     5401 cgtccttgtc caccttccaa gcgttgccgt aggttccgtc atacctcgga acgatgccca
     5461 tcggtcccca acccatgaac tcgcaagcct ccacaaatac agatggcaac ccttcgaatc
     5521 gcggaagttc taggcgaccg tgccggtccc gcactccgag ttccccccgt tccaactggc
     5581 gtaggcgccg gatgcatatc gatggaatcc agagaccggc ctggtgacac tctttgagca
     5641 gccctctgag ccataggatc aggtttggct gacggttctg gatcttgtac cgggaaacga
     5701 gccaagcttg ttcaactccc tgcacaaaca acctcaaccg cgagcggggc actccagggt
     5761 tggtcatctc caagagggat ttgtcgatgg caagtgtctc aagaagcgtc tttcggattc
     5821 gcaagtcttc cccggccgat aaaggaaaca aacatcgttt cgcatatagt tgggtcgtct
     5881 taggttttga ctttactttt tttagttgca gcccagcttg ctcacctgtc atcaagtcac
     5941 ggctcataaa ttcttcatcc cttcgagggt ggttgccttc ccctgggaca cctttggggc
     6001 ccgaatcttg gcccgcggtc ggccgttccg ccgtggagtg tccgccgggg ccgaggtgtc
     6061 ctgcgggcct ggaagtgacc agcccattcg aaactccttt cggccccaca caccttcctc
     6121 cgatcgttgc tgcgcc
//
LOCUS       DASYVO010000180         6129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_132886, whole genome shotgun sequence.
ACCESSION   DASYVO010000180 DASYVO010000000
VERSION     DASYVO010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132886"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            644..2905
                     /locus_tag="VGR03_06060"
     CDS             644..2905
                     /locus_tag="VGR03_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV1993874.1"
                     /translation="MNLSRTKNLDDPDSARAEALHASLQGIERREWWLWGVAGIITLL
                     LTVALASFILPHSPVHEDFYSVYVLPQAIRGLVALVFLFDLYAIYQHLQIHRIRRQLI
                     EREELFRLISENVADMIAVVDMKGQRIYNSLSYQKVLGYSPTELQASSAFEQIHPDDR
                     ERVKEAAEEARQTGKGETLEYRFRHKNGTWLVLESTSSVIQDARGDPEKLVIVNRNVT
                     KRKNAEEALRRSETSFRSMVEDAPYGIYRASPTGHFLQVNPALRRMLGYGSAEELRKA
                     SIESDIFRHVGEYQRVNELLARSEEFKDVEMEWKQHDGTPITVRCSGRRVNDENGVPA
                     HFEVLAEDVTERRVLERQLRMAQKMEAIGRLSGGIAHDFNNLLQVIIGYSGVLKKSLG
                     ATNPLYEHALEVEKAGQRAASLTKQLLAFSRQQVLTPAVLNLNALVSDMGKMLPRLLG
                     EDIEMSLELEPELGNVKADQSQIEQVIMNLAVNARDAMPVGGKLKIQTANVELDQAYT
                     RNHPGSKAGHYVELAVMDTGTGMNAETLAHIFEPFFTTKERGKGTGLGLATVYGVVKQ
                     SNGYIWVDSEPGKGASFQIYLPLHAAQDPVTEPEADSWEKLQGSETILLVEDADPLRK
                     LAQVILEANGFRVLSAQSGEEALEVAARYGGTFDLLLTDVVMPGMNGRVLAEQLSPRQ
                     PGMKVLYMSGYTDSFIAGHGVLDPETHLLHKPFTEEILVRKVRDVLDGGIKTPGASGA
                     VAGPAGSGNRTSR"
     gene            2919..3584
                     /locus_tag="VGR03_06065"
     CDS             2919..3584
                     /locus_tag="VGR03_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV1993875.1"
                     /translation="MGKLLVVDDDEVLRRLMRLELGDTYEIMETGEPEQGLALALEHK
                     PDAILLDLRMPKYSGYELCQTFTSFSRTQTIPVIIVSGETGGQTKEYCKQLGATDYFE
                     KPIDFQALRKCLAEVAKPQRHVPRSEARVRLRVPLKLRGKDRQGNKVETDTTTENVSV
                     SGFLCVSTADLAAHSIVEVFLGSCGEKYVGKALVLHSDGKDAVIRRYGCHFIEKTGPW
                     VLQ"
     gene            3662..4984
                     /locus_tag="VGR03_06070"
     CDS             3662..4984
                     /locus_tag="VGR03_06070"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1993876.1"
                     /translation="MTEKQERTGLLLSALVLFGINVLNFYDRNALGALVEPLRKEFHL
                     TDAQNGFLSSAFVWVYAIVGLPLGWLADSRSRKKLLAGAVFVWASLTACAGFATSFAL
                     LLISRLGVGVGEAACAPVGTSWLGDLFPPEKRSRALAFFMLGVPIGGALGFFLTGPIA
                     QAYGWRRAIVVAAAPALLLIPALLALREPKRGAAEVRKTPLGRGSVWSVLRIRTFWWI
                     IASGALLNFNMYVVGTFLPAFFSRVHHLSLALSNVATGVVYLLGGVSGGLLAGHLGDS
                     VFRQRKDGRLRLSAVMVLLAAPLALLGFLQPQSSRLLAIALLVLVYGAMTTYYGFVYS
                     SLHDIVSPGQRGTAMAVYFFGMYLCGASYGPLLTGRLSDHLAHRAAGLAGSAVVTEAF
                     RAIGLQRAMLVIPAFCIPLAFVLYMASRTIIADIEARHAAEASAPHSG"
     gene            4997..5152
                     /locus_tag="VGR03_06075"
     CDS             4997..5152
                     /locus_tag="VGR03_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993877.1"
                     /translation="MKARRAKLDMGKEARRRARKAGSAPAATRVIPDKRKKLVKHKKR
                     WLERELD"
     gene            complement(5210..5704)
                     /locus_tag="VGR03_06080"
     CDS             complement(5210..5704)
                     /locus_tag="VGR03_06080"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV1993878.1"
                     /translation="MTTILAGKVAPGFALKGLDGKNYSLTPLLGRGPVVAAFFKISCP
                     VCQFTFPFLERLHNRYGGDGVTILGVSQDDVRDTEEFCREYGVTFPVVIDGDGYPVSN
                     AYGLTNVPTILLIEAGGKVRVSSMGFDRKDLEAMSTGLAERRRIAASPLFLPDEIIPE
                     QKPG"
     gene            complement(5802..>6129)
                     /locus_tag="VGR03_06085"
     CDS             complement(5802..>6129)
                     /locus_tag="VGR03_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993879.1"
                     /translation="LRQKKDMRQIVMITDGKPSALTLEDGRIYKNAFGLDPFVVAQTL
                     EEVNKCKRAGIVINTFMLASDYSLVHFVQKITEMCRGKAYFTTPFTLGQYLLMDYMSR
                     KTRQIH"
BASE COUNT         1278 a         1712 c         1930 g         1209 t
ORIGIN      
        1 tgggccaggt ggcggcacca tggtgggcac catcatcgct tccgctggag tcacgttttc
       61 taccgccgac aacgcagtca taaccacgct cgatggcaga gcgttggggc tcaatgcgtc
      121 ggtgaccatg gtcaacaccg tgatcaatgt acccgctcca taaaaaagaa tttatttgag
      181 aaggaatcac aaactcgctt ggtggatgca cagggaagca atggacaaat tggcgtgaat
      241 tctgttccca acttggaacc ggcgaccgaa gtaattcagt aacctgcgcg caatatggcc
      301 tgggtaggac tcccacaggc ttccgccaag accccgctga taccgttggt cttccttccc
      361 ttgaatctgc cgagaggacg cgccagggag ctgttccaaa ctgccactcg cggcccacga
      421 tggcacggga acgccacgcg atccgtttgc ccagtttccc ctggcagcct atctgcttat
      481 ggttcaatta cttacaggac ataatcagtg caagagggag gacttcttgg cggtcgtcgt
      541 gcatgaaagg gttgcgaccg agaagcgacc aacgaccagc gcaagagggg atgaaataag
      601 agcaccgttg cgaggggaag gacagagcgc agacgcctga accatgaatc tgagtagaac
      661 gaagaacctg gacgatcccg acagcgcgcg cgcggaagca ctccacgcga gcctccaggg
      721 gattgagcgg cgggaatggt ggttgtgggg agtggcgggg attatcacac tgctgctcac
      781 ggtcgcgctg gcctctttta ttctccccca ttcgcctgtt cacgaggatt tttactcggt
      841 atacgtattg ccgcaagcca tccgagggct ggtggcgctg gtcttcttgt tcgatctgta
      901 cgcgatctat cagcatctgc agatccacag gatccgccgg caactgatag agcgggagga
      961 attgtttcgc cttatcagcg agaacgtagc ggacatgatt gcggtcgtgg atatgaaggg
     1021 acagcggatt tacaacagcc tttcctatca gaaggtgctg ggatattcgc ccacggagct
     1081 gcaggcttcc tcggctttcg aacagattca ccccgatgac cgcgaacgcg tgaaggaagc
     1141 agcggaagaa gcgcggcaaa ccggaaaagg ggaaacattg gagtaccgct tccgccacaa
     1201 gaatgggacc tggctggtgc tggaatcaac ctcaagcgtt atccaagatg ccaggggcga
     1261 tccggaaaag cttgtgatcg tgaaccggaa cgttacaaag cgcaaaaatg cggaggaagc
     1321 cttgcggcgg tcggagacga gtttccggtc gatggtggaa gacgcaccct acgggatcta
     1381 tcgggcgagc ccgaccggcc attttctgca agtgaatcct gcgctccgga gaatgcttgg
     1441 ttatgggtcc gcggaagaac ttcgcaaggc cagtatcgag agcgatatct tccgccacgt
     1501 gggcgaatat caacgagtga acgaactcct cgcacgaagc gaagaattca aagatgtcga
     1561 aatggagtgg aagcagcatg acggcacgcc gatcacggtg cggtgttcgg gccgccgggt
     1621 gaatgacgag aacggggtgc ctgcgcactt cgaggtactt gccgaggacg tgacggaaag
     1681 gcgcgtgcta gagcggcaac tgagaatggc ccaaaagatg gaggccattg gccgactctc
     1741 ggggggaatc gcgcacgatt tcaacaatct cctgcaagtc atcattggat atagcggagt
     1801 gctgaagaaa tcgctgggcg caacgaatcc gttgtacgag cacgcgctgg aagtcgaaaa
     1861 ggcggggcag cgcgccgcct ccctgacgaa gcagttgctc gctttcagcc ggcagcaggt
     1921 cctgacgccc gcggttctga acctcaatgc actcgtctcg gacatgggga agatgcttcc
     1981 gcgcctgctg ggagaggaca tcgaaatgtc gctggagctg gagccggaac ttggaaatgt
     2041 gaaggcggac caaagccaga ttgaacaggt gatcatgaac ctggcggtca acgcgcgtga
     2101 cgccatgccg gtgggcggca aactgaagat ccaaactgcc aacgtggagc tggaccaggc
     2161 gtacacgcgg aatcatccgg gctccaaggc agggcactac gtggagctgg cggtgatgga
     2221 cacggggacg ggaatgaacg ccgagacgct tgcgcatatt ttcgaaccgt ttttcaccac
     2281 gaaggaacgg ggaaagggca cggggctagg gctggcgact gtgtacggcg tggtgaagca
     2341 gagcaacggg tatatctggg tggatagcga gccgggaaaa ggcgcgagct ttcagattta
     2401 cctcccgctt catgcggccc aggatccggt gacggaaccc gaggccgatt cgtgggagaa
     2461 actgcaaggg tcggagacca ttctactggt ggaggacgcc gacccactgc gaaagcttgc
     2521 gcaggtaatc cttgaagcca acgggttccg cgtgttgtcg gcgcagagcg gtgaggaagc
     2581 tctcgaggtg gccgcgcggt acgggggaac ctttgacctg ctgctgaccg atgtcgtgat
     2641 gccggggatg aacggccgcg tgctggcaga gcaattatcg ccgcgccagc cgggaatgaa
     2701 ggtgctctat atgtcaggct acacagacag cttcatcgct gggcatggcg ttctggatcc
     2761 tgaaacccac ctactgcata aaccattcac ggaagagatc cttgttcgga aagtgcgcga
     2821 cgtgcttgac ggtggaataa agacaccggg cgcttcaggg gcagtggcag gtcctgccgg
     2881 aagcgggaac cgtacgagcc gataggagaa agcaatcgat gggaaaactg ctggtagtgg
     2941 acgacgatga ggtgctgcgc cgcctgatgc gccttgagct gggcgacacc tacgaaatca
     3001 tggagaccgg ggagcccgag cagggtctgg ctctagccct cgagcacaag cccgacgcca
     3061 tcttgctgga tctgcgaatg ccgaagtatt ccggctatga actgtgtcag acattcacct
     3121 ctttcagccg gacacagacg attcccgtga tcatcgtgag cggcgagacg ggcggacaga
     3181 ccaaagaata ctgcaagcag ctcggcgcaa cagactattt cgagaagccg attgacttcc
     3241 aagcgctgcg aaaatgcctt gccgaagtag ccaagcccca gcggcacgtc cctcgaagcg
     3301 aggcgcgcgt gcgattgcga gttccgctga aactgcgggg caaggacagg caagggaaca
     3361 aggtcgaaac ggacaccacg actgagaacg tgagtgttag cggctttttg tgtgtttcca
     3421 cggcggactt ggcggcccat tccatcgtgg aggtcttcct cgggagttgc ggcgaaaagt
     3481 acgtaggcaa agcactggtc ctccactcgg acgggaaaga tgcagtaatt cgccgttacg
     3541 gatgccactt catcgagaag accggtccct gggtgttgca gtagccttcg tcattcaatt
     3601 ttctttttaa aggctgaggc gactgcgggc ttcgctgcgg gtgtgttagc atcccgggac
     3661 catgaccgag aagcaggaga ggaccggcct gttgttgagc gcgctggtcc tgttcggaat
     3721 caacgttctg aacttttacg accggaacgc actgggtgcg ctggtcgagc ccctccgcaa
     3781 ggagtttcac ctaaccgacg cgcagaatgg tttcctgagc agtgcattcg tctgggtgta
     3841 cgcaatcgtc gggcttcccc tcggctggct tgcggactcc aggagccgga agaagctgct
     3901 tgccggagcc gtgttcgtct gggcttcgct cacggcctgc gcgggtttcg ccacaagttt
     3961 cgctttgctt ttgatctcgc gtctgggcgt gggtgtcggc gaagcggcgt gcgctccggt
     4021 cgggaccagt tggctcggcg acctcttccc gccggaaaag cggtcacgcg ccctggcgtt
     4081 ctttatgctc ggagtgccaa tcgggggagc gctgggcttt ttcttgaccg gccctatcgc
     4141 ccaggcgtac ggctggcgcc gggcaatcgt tgtggctgca gctcctgcac tgctgctgat
     4201 tccggcgctg cttgcgcttc gcgaacccaa gcgcggcgct gcggaggtcc gcaaaactcc
     4261 cctcggcagg ggctccgtct ggagcgtcct gcgcatccgg acattctggt ggatcatcgc
     4321 ctcgggcgcg ttgctcaatt tcaacatgta cgtcgtcggt acgttcctgc ccgcgttctt
     4381 cagccgcgtc caccaccttt cgctcgcttt gtcgaatgtt gccaccggcg tggtctacct
     4441 ccttggcggc gtcagtggag gtctcctggc cggacacctg ggtgattccg ttttccgaca
     4501 gcgcaaggat gggcgcctgc ggctttcagc cgtcatggtc ctgctggccg cgccgctcgc
     4561 gttattgggc tttctccaac cgcaaagctc gcggctgctg gccatcgcgc tgcttgtcct
     4621 ggtatacggc gcaatgacga cgtactacgg cttcgtttat tcctctctcc atgacatcgt
     4681 ctctccggga cagcgcggca cggccatggc cgtatacttc ttcggaatgt atctgtgcgg
     4741 cgcctcctac ggcccgctgc tcacggggcg tttgagcgat cacctggcgc atcgcgcggc
     4801 cgggctggcg gggtccgcgg tggttaccga ggcgttccgc gcaatcggtt tgcagcgggc
     4861 gatgctggtc atcccggcgt tttgcattcc gctggccttc gtcctttaca tggcgtcgcg
     4921 gacgatcatc gcagacatcg aggcgcggca cgctgccgaa gcctccgccc cgcactccgg
     4981 gtaatcagga gaaaccatga aggcgcggcg tgcgaaacta gacatgggca aagaagcgcg
     5041 gcggcgcgcg cgaaaagctg gctcggcgcc ggccgcaacc cgtgtgatcc cggacaagcg
     5101 gaaaaaactc gtcaaacaca agaagaggtg gctggagcga gaactcgact gagactgtgt
     5161 cttttcgcga atgcgttttg ggtccggacc ggacctagtt cttggatcct caaccgggtt
     5221 tctgttccgg aatgatttcg tcgggcagga aaaggggaga cgccgcgatc ctgcgcctct
     5281 cggcaagccc cgtggacatg gcctccaaat ccttcctgtc aaatcccatg ctgctcaccc
     5341 ggaccttgcc gccggcttcg atcagcaaga tcgtcgggac gttggtgagg ccgtaggcgt
     5401 tcgaaaccgg atagccgtcg ccgtcgatca ctaccgggaa ggtgacgccg tattcccggc
     5461 agaactcttc cgtgtcgcgg acgtcatcct gagaaacccc gagaatcgtc acaccgtccc
     5521 cgccatagcg gttgtgcagg cgctcgagaa atgggaaggt gaactggcac acggggcagg
     5581 agatcttgaa aaacgcagcg accaccgggc cgcgcccgag cagaggggtc aaggaatagt
     5641 tttttccatc gaggcccttg agggcgaaac ccggcgcaac cttcccagcg agaatcgtgg
     5701 tcatcgaagc ttccctctcc gatacatgcc aagcctgcgg cagcgatctc cttggaaaac
     5761 gataccgcat tcgcgaatcg ccgccaaatc gggagcccgc ttcagtgaat ctggcgcgtc
     5821 ttccggctca tgtagtccat caggagatac tggccgagcg taaagggcgt cgtgaaatag
     5881 gctttgccgc ggcacatctc cgtgatcttc tggacaaaat gaacgaggct gtaatcgctt
     5941 gcgagcatga aggtgttgat cactatgccc gcccgcttgc acttgttcac ctcttcaagc
     6001 gtttgcgcaa caacaaaggg gtcgagaccg aaggcattct tgtagatgcg gccgtcctcc
     6061 agggtcaggg cagagggctt gccgtcggtg atcatgacga tttggcgcat gtccttcttc
     6121 tgccgcagg
//
LOCUS       DASYVO010000181        11418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_161469, whole genome shotgun sequence.
ACCESSION   DASYVO010000181 DASYVO010000000
VERSION     DASYVO010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 11418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11418
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161469"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..828
                     /locus_tag="VGR03_06090"
     CDS             130..828
                     /locus_tag="VGR03_06090"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV1993880.1"
                     /translation="MPEKHLLLRVLNEEIVGCRQCPRLVKHREKMAREKRRAYREWTY
                     WGKPVPGFGDAGAQLLILGLAPAAHGANRTGRMFTGDRSGDFLYAALHKAGMANQATS
                     LHRDDGLQLKNAYISATCRCAPPDNKPLPEEIANCSGYLAREMEILKPKAILALGKIA
                     WDAYLEILKQRGVIASRALYKFAHGAEAEVAAGFPRLFGVYHPSQQNTQTGRVTPAMY
                     AAVLRRIQRFLEKS"
     gene            1119..1859
                     /locus_tag="VGR03_06095"
     CDS             1119..1859
                     /locus_tag="VGR03_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993881.1"
                     /translation="MERMKLKSAFAGVVILTMTLALGVACNAQDQTQEPSREATTQAP
                     DQQRQKADGERHGLPVETFAVAPGTKFLVRLEDELGTKGTKENEKFKVKTLEPLEAGS
                     GIYLPPGAEIRGHISHVEPAGVAGRAKIWLTFDEIHTKFGTLPIVAEVVSVPGDHSVK
                     SGATQEGLIEGRSSNQHDAAEAAAAGAAIGAVKGVKDKDKKEAAEGAALGALTAYLME
                     SGRGHELNLPKGAKLELELERALYLVKE"
     gene            2326..3519
                     /locus_tag="VGR03_06100"
     CDS             2326..3519
                     /locus_tag="VGR03_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993882.1"
                     /translation="MSRQFVEFRMKYIAGAFLAGLAAISGVGAQSQDFDPTLSKDHPV
                     LELDLGKFGYDTSSGTRRLPKFVDFTDLNRLALGWLTLDDPTLADKTGPLTARPAHLH
                     VLVLDARTGRKEGLQEWSTPSTPVRFAGVGGGKFLICTASTLRLFSASFEVIREENLP
                     GDRACFNPFLSEVWGISPSKRYLLLSSFSAPTYQNTLFDVETFTSVANWTEKRRTNDV
                     SDHWLAALCGEQGEMCIREMDQPWRAFHPEGLDKQLNGFKPKSPFFVNDRLLVIEAWN
                     EMAAVDVDGQVLFQVRLPKKRSFGQPTRSTGGGRFAIIENRQRGVTNQALDMYAFASN
                     DRVVVFSIPDRRAIYAVGVKGRSPWAPWEAHRNYVALSPDGALLAVAAGGLLKVYRLP
                     ERNSR"
     gene            3811..5235
                     /locus_tag="VGR03_06105"
     CDS             3811..5235
                     /locus_tag="VGR03_06105"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV1993883.1"
                     /translation="MGEETRAAVRAIEMVERKDRVLIVEDEDNARKGYEQLLQRWGCD
                     VVGVATAEEALAKFASYQPDTLIADVELPGMNGLDLLKHLGPELYDVPAIIITGKGSE
                     ERAVAAIEAGAFWYIEKPLKGPVLRALLDRALGKARDARQLTVLQRQLRETGRLGDLV
                     GGSKPMQEVMRIVEMAAPSSASVLITGETGSGKEIVARTLHKLSPRANGPFIAINCSA
                     IPETLMESEIFGHERGAFTGAAERRIGCFELADAGTLLLDEIGEMPAQTQAKLLRVLE
                     DRKVRRLGSKSETPVDVRVLAATNKEPEQAVSSGHLRQDLYFRLNVFHIHLPPLREHK
                     DDIPMLVEHILRDVNAKHGKHVRGIGAEVLDIFMGHTWPGNIRELRNVLERATIMCEK
                     DLITRACLPGEFGKTAAKGPSDLSAIKFPVGTTVDAMERVLILQTLGATGNNKTRAAE
                     LLGISLKTLHNKLKEYGGDRAETE"
     gene            5245..7101
                     /locus_tag="VGR03_06110"
     CDS             5245..7101
                     /locus_tag="VGR03_06110"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1993884.1"
                     /translation="MQLRLRTKLTLVMTGLVLLVAAVLSGVFAAQLLEQLLQATSVRA
                     NDLANQVFLQAQHALTDAGAQGLRPASDTAQEIHDYVRHAFEINEGLRTQLKAAKDNP
                     LIYEVSIADHDGMVLISTDETMPGKFLPRRTPLSQLVQRNFLHQVKVLAGPSKVFELD
                     YPFSKGGQPFGEVRVALDSGILLKEIEPSLRTSATFVLIALVISTLLAAIVSRATLAP
                     LHDIAAQLDRISAGQYDAPSPEVTGFAGSGDELGLVSRKITQVGQQLRGVHEIFSTMR
                     ENMNSVMAGLEDGLLLFTRDSRAVMISPAAEKFLGAPAGQFLGRRVTEIFPPGHPLHE
                     ALHIESDELSEVAAETELQTSEGPRRVSVTVQAIQEDGERMGALVTLRDLDSLESINT
                     QLQVSERLAALGRITAGVAHEVKNPLNSMRLWLENLKESLPADADGASQQAVQVLDKE
                     IDRLDAVVKRFLDFTRPMDIRLEATQLAELLKEVLEVAQQQFQKSNIQVAQLLPIDVP
                     EVYVDRALLKQAVLNLVLNAAEAMPSGGQLRLVLSRRGEMAEITVGDTGKGIPLENRQ
                     KIFQLFFTTRPGGSGIGLASTFRIVQLLNGSIDFTSEVGRGTTFRIELPIAA"
     gene            7104..7700
                     /locus_tag="VGR03_06115"
     CDS             7104..7700
                     /locus_tag="VGR03_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993885.1"
                     /translation="MTENPVRSLMVLPRGFLQSGIFLLCCAACVLGGCSARKRPSIPW
                     VTAVQVRPVTQAHTAAADSASEDLLPELRLELPPFPGHLITVRTAPPRPRVSTPPSAT
                     TGNDAEKLDAPMIAPQLTAQESAVAQQQTNQSLGIAEKNLESARGKNLNAAQSDLVSK
                     IRGFIKDAREAAQITDWSRARSLAKKAQVLSEELAGSL"
     gene            7802..8941
                     /gene="thiO"
                     /locus_tag="VGR03_06120"
     CDS             7802..8941
                     /gene="thiO"
                     /locus_tag="VGR03_06120"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEV1993886.1"
                     /translation="MKKFDVAIAGGGVIGGAIALELVRAGLRVAVFDRQQPGQEASWA
                     SAGILSPAPENPRMIAMVPLGKASLAIYPEFVAQVEEISGKSTGFRPKGTLEALFSHD
                     TKAELSTIIALHHGLGLKAEPLRAEDARELEPALSEEVEAAVLRPEEASVDNRLLTAA
                     ILEAAQRSGAEIFPGNGAKAIWREGNRCAGLVLQNEKVEAKWTIIAAGCFSATIEGIA
                     PYAPVRPAKGQMAALRADDLKMDRVLWSEKIYLVPRNDGRIVAGATVEYAGFDKRTTA
                     GGIEKILSAAIDLAPGLANARIEETWAGLRPDSPDHLPILGPTDIDGLLMATGHFRSG
                     ILLTPITACLIREWITEQRVSVDWDRFSPLRFQSAATDAARHAPA"
     gene            complement(9019..9348)
                     /locus_tag="VGR03_06125"
     CDS             complement(9019..9348)
                     /locus_tag="VGR03_06125"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEV1993887.1"
                     /translation="MKILIDECLPDELKESVAAMGHECQTARRAGYGSKKNGELLALA
                     EGQWDVLLTSDRNIKYQQNMAGRTVSILILRAKSNRLKDLLPLMPACAEGLLSIQPGR
                     VVEVGPL"
     gene            complement(10040..11023)
                     /locus_tag="VGR03_06130"
     CDS             complement(10040..11023)
                     /locus_tag="VGR03_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993888.1"
                     /translation="MEDTDKTPDQPPSPPPPKAAAAPSPKSRKANISPAEREIFERYG
                     ETVIAMQVAGSFDQNVEHARDWLTERTRSRQRREQITFALEIVVVLLIGGEILLSLWG
                     GHQQSQNFDKQQQILTNLQNSSAATAKTLTSLQSATESMNTILQMQLDAAKKSEAQAE
                     RSAKAGEASASTASQALHVSQRAYVHITASLLKPPAAGERVHPIATIANVGRTPALDV
                     GVLLFFSFAPVSTTTKTARDIASSYPQRGPTNSVFTLPSGQTSTMQTDSQAPYLQSMV
                     DQMTNGEAVLYVFATASYKDVFNQPHHTEICGFYQPKLNIFGACTEYNKSD"
BASE COUNT         2510 a         3029 c         3615 g         2264 t
ORIGIN      
        1 ctgcgctcgg actcgttcgg cggattggga aagaatggat ggagagtgga acgtacaatt
       61 ccctgctcga tggcgcgatc ccgttcgccg atgtgaatcg ggtgatggga cgcccgatct
      121 cgtgacatca tgcctgaaaa acatttgttg cttcgcgttc tgaatgagga aattgtgggg
      181 tgccgtcagt gtccgcgctt ggtgaaacat cgcgaaaaga tggcgcggga aaagcggcgt
      241 gcgtatcgcg agtggaccta ctggggaaaa ccggtcccgg gattcggaga cgctggcgcg
      301 cagcttttga tcctgggact ggcaccggca gcgcatggcg cgaatcgcac ggggcgcatg
      361 ttcacgggcg accgctctgg agattttcta tatgcagcgc tgcacaaagc agggatggcg
      421 aatcaggcga cttccctgca ccgcgacgat ggactacagc tgaagaacgc gtacattagc
      481 gcaacgtgcc gatgtgcacc gccggacaat aagccgttgc cagaggagat tgcgaattgc
      541 agtggttacc tcgcgcgtga gatggagatt ctgaagccga aggcgatctt ggcactcggg
      601 aaaatcgcct gggacgcgta cctcgaaatc ttgaagcaac gcggagtgat tgcatcgagg
      661 gcgctctaca agtttgcgca cggcgctgag gctgaagttg ctgccggctt tccgcggctg
      721 tttggggtct atcaccctag ccagcaaaac acgcagaccg gccgcgtcac tccggcgatg
      781 tatgccgccg tactgcgccg gatccagcgc ttcctcgaaa agagctagaa gcgcggcccg
      841 cctccccgac aagagtcggg gtaaacgcca ggcgggccct acagagcgca cgcagacgag
      901 cccgattctc gtgagaaagc cggactaaac cgaaaaagtg tcgggaaagc cttgcggggc
      961 gatggcggga ttggccgaca gaagtgtcgc catctaagtt gagtgtcagg tggcacctcc
     1021 aagtgcacgc ttttcaatcc tttgcagcac ctctcgcgag tcggctgtgg ccgtccatgt
     1081 gcattcattg actgctcagc atttcgcggg aggagaccat ggaacgaatg aaactcaaga
     1141 gtgcattcgc cggcgtcgtg attttgacga tgacgcttgc tttgggagtc gcatgcaatg
     1201 cgcaggatca gacccaggaa ccgtcacgag aagcgacaac ccaagcacca gaccagcagc
     1261 gtcagaaggc cgacggagaa cgacacggat tgcccgttga aactttcgcc gtcgcgccgg
     1321 ggacgaagtt tctggtgcgg ctcgaagatg aattgggtac caagggcacg aaggaaaacg
     1381 aaaagttcaa agtgaagacg ctcgagccgc tcgaagcggg cagcgggatt tatctgccgc
     1441 caggggcgga gattcgcggg catatcagcc acgtggaacc agcgggagtg gcgggacggg
     1501 cgaaaatctg gctcacgttc gacgagattc atacaaaatt tggcactctg ccgatcgtcg
     1561 ccgaagtcgt aagcgtgccg ggggaccaca gcgtgaaatc cggcgcgacg caagaaggat
     1621 tgatcgaagg gcgatccagc aatcaacatg acgctgccga agcagccgct gcgggcgcgg
     1681 cgatcggcgc cgtgaagggc gtgaaggaca aagacaagaa agaagctgcg gaaggcgccg
     1741 cgctgggagc gctcacggcg tacctgatgg aatccggccg cggccacgag ctgaacctgc
     1801 caaagggcgc aaaactggag ctggaactgg agcgcgcgct gtatctcgta aaggaatgag
     1861 gctgaacttt ctcgatcagg tgaactttct cgatcattgg acagcgttcc aaaatgggaa
     1921 gagtaccgcc cccccacacc cccgtgtttt ttgtcgcaga gtgcgcaatt cattgaaaag
     1981 aagagagtta agttttttag aaatgcagaa aagtgcaaga gagtgcgcaa gaatatgaaa
     2041 ggaaagggga tagggggtag gaaatagggg atagtggccc cgaagagccg gcacccccta
     2101 caccccccac caatttacat gaatatcaaa agaaaagact tacggaatga tcgtttttca
     2161 aatgattgat tctaaagact atgtttttgg ttgtttagca agagccagga ggcggctcca
     2221 aaaaaagaaa agcgggagca gagctcccgc actccaaatg gagttttcta tgacatcaat
     2281 tataccaaag ggtagtggga agtcaaggag aaaaagaggg acaaaatgag ccggcaattt
     2341 gtagagttca ggatgaagta tatagctggc gcattcttag cgggacttgc tgccatttcc
     2401 ggcgtaggag cgcagtcgca agacttcgac ccaaccctga gcaaagacca cccggtctta
     2461 gagttagatc ttggcaaatt cggctacgac acttccagcg ggactaggcg cctacccaaa
     2521 tttgttgact ttaccgattt gaaccgactc gccttggggt ggctgacgct tgatgatccc
     2581 actctggcgg ataaaaccgg ccctctgacc gccaggccag cgcatttgca cgtccttgtt
     2641 cttgacgcca ggacgggccg gaaagaaggc ttgcaggagt ggtctacgcc atccaccccg
     2701 gttcgcttcg cgggagtcgg cggaggtaag tttctcatct gcaccgcgag cacgctgcgt
     2761 ttgttctcgg ccagctttga agtaattcga gaagaaaatc tgccaggtga ccgtgcttgt
     2821 ttcaacccct tcctctccga agtatggggg atttcaccaa gcaagcgcta tttgctgctt
     2881 tcctcttttt ctgcaccgac ttaccagaat actctgttcg acgtcgaaac gtttacttct
     2941 gtagccaact ggacagaaaa aagacgaaca aatgacgtct cggaccactg gctggccgca
     3001 ttatgtggcg aacagggcga gatgtgtatc cgggaaatgg accagccgtg gagagcattt
     3061 catcccgaag gcctcgacaa acagctgaat ggcttcaagc ccaaatcgcc gttcttcgta
     3121 aacgacagac tgttggtaat cgaagcttgg aacgaaatgg cagcagtaga cgtagacggc
     3181 caggtgttgt tccaggtgag actgcccaag aaacggtcat tcggccagcc gacgagatca
     3241 accggaggtg gaaggtttgc aatcatagag aataggcaaa gaggcgtgac caatcaggcg
     3301 ttggatatgt atgcgtttgc ttccaacgat cgggtagttg tatttagcat tcccgaccgc
     3361 cgcgccattt atgcggttgg agtcaaaggc cgatcaccgt gggccccgtg ggaggcacat
     3421 aggaactacg tggccctctc gcctgacggg gcccttcttg cagttgccgc tgggggtttg
     3481 cttaaggttt atcggcttcc tgagcgtaac tcccgctaag cttgctcatt ggcagtcgga
     3541 aagatgcttg cagtcatcag cggcgtggga ctctccgtct tcgagattcc ttgagaatcc
     3601 gcgaatgctc tcgtaaacgt gaatacataa caaaaaaggc tgcgccccag tctgtaaatc
     3661 gctcagcgtg aggcacacag ccagacctcg cccgctttgg agaatcccgg aaaacttggc
     3721 aaggggctgg cagattgtta gagtcgaggt gtccagcaaa tcagtgcgta agccattcat
     3781 caagaacatg ccgaagagag gctgagaccc atgggggaag agacgcgggc ggcggtgcga
     3841 gccatcgaaa tggtggagcg gaaggaccgc gtgctgattg tcgaagatga agacaatgcg
     3901 cgaaagggct acgaacagct cttgcagcgc tggggctgcg acgttgtggg tgtggcaacg
     3961 gcagaagaag cgctggcgaa atttgcgagt taccagccgg acacgctgat cgcggacgtg
     4021 gaattgcccg ggatgaatgg gctggacctg ctgaagcacc tcgggccgga actctacgat
     4081 gtgccggcca tcatcattac cggcaagggc agcgaagaac gcgcggtggc ggcgatcgag
     4141 gcgggagcgt tctggtacat cgaaaaaccc ctgaaggggc cagtgctgcg cgcgctgctg
     4201 gaccgtgcgc tgggcaaggc gcgagatgcg cggcagctca cagtgctgca gcggcagctc
     4261 cgcgaaacag gacgcctggg cgacctcgtc ggggggtcga agccgatgca ggaagtgatg
     4321 cgcatcgtgg agatggccgc gccgagctcg gcgtcggtgc tgatcacggg agagaccggg
     4381 tcgggcaagg aaatcgtcgc gcggacgctg cacaagcttt cgccgcgcgc caacggaccg
     4441 tttatcgcga tcaattgttc ggctatcccc gagacgttga tggagagcga gatttttggc
     4501 cacgagcgcg gggccttcac gggcgcggcc gagcggcgca tcggctgctt cgagcttgcg
     4561 gatgcgggaa cgctgctgct ggatgaaatc ggcgagatgc ccgcgcagac gcaggcgaag
     4621 ctattgcgcg ttctggaaga ccgcaaagtg cgccggctgg ggagcaagag tgagactccg
     4681 gtggacgtgc gcgttctcgc ggcaaccaac aaagaacccg aacaggccgt cagcagcggc
     4741 catttgcggc aagatctcta ctttcgcctg aatgtgtttc acatccacct gccgccgctg
     4801 cgcgagcaca aggacgacat tccgatgctc gtcgagcata ttctgcgcga cgtcaacgcc
     4861 aagcatggga agcatgtgcg cgggatcggc gcggaagtgc tggacatttt catggggcac
     4921 acgtggccgg gaaatatccg cgagctgcgc aacgtgctgg agcgcgctac gatcatgtgc
     4981 gagaaagatt tgattacacg cgcgtgcctc ccgggagaat tcggaaagac ggcggcgaaa
     5041 gggccgagcg atctttcggc gatcaagttt ccggtgggaa caacggtgga tgcgatggag
     5101 cgcgtgctta tcctgcagac gcttggcgca accggaaaca acaagacgcg ggcggcggaa
     5161 ctgctcggca tcagcctgaa aacacttcat aataagctga aagaatacgg cggcgatcgc
     5221 gccgaaacgg agtaggaacg cagaatgcag ctgcggctgc ggacaaaact cacgctggtg
     5281 atgacggggc tggtgctgct cgtcgcggcg gtcttgtctg gcgtattcgc ggcgcagctc
     5341 ctcgagcaac tcctgcaggc caccagcgtg cgcgccaacg acctggcgaa ccaggttttt
     5401 ttgcaggcgc agcatgcgct aacggatgcc ggggcccagg gtttgcggcc ggcttcggac
     5461 accgctcaag aaatccacga ttacgtaagg cacgctttcg aaatcaacga aggcctgcgc
     5521 acccagctca aagccgcaaa agacaatcca ctcatttacg aagtttcgat tgccgatcat
     5581 gacgggatgg tgctgatttc aaccgatgag accatgccgg ggaaattttt gccgcgaaga
     5641 actcctcttt ctcaattggt gcagagaaat ttcctccatc aggtgaaagt tcttgcggga
     5701 ccgtcgaagg ttttcgaact cgactatccg ttcagcaaag ggggccagcc gttcggggaa
     5761 gtccgcgtgg cactggactc cggaattctt ttgaaagaga tcgagccaag cttgcgcacg
     5821 tccgcgacct tcgtgctgat cgcgctggtg atttccacgc tgcttgcggc gatcgtgagc
     5881 agggcgacgc tggctccctt gcacgatatc gccgcacagt tggaccgtat ctcggccggg
     5941 caatacgacg cgccttctcc ggaggtcact ggcttcgcag gaagcggcga tgaactggga
     6001 ctggtgagcc ggaaaattac acaagtcggc cagcaactgc gcggagtgca tgaaatcttc
     6061 agcaccatgc gcgaaaacat gaattccgtc atggccgggc tcgaggacgg cctgctattg
     6121 ttcacgcggg attcgcgcgc cgtaatgatc agtcccgcgg cagaaaaatt tctgggcgcc
     6181 cccgccggcc aattcctggg gcgtcgcgtg acggagattt ttcctcccgg tcatcccctg
     6241 cacgaggcgt tgcacattga aagcgacgag ctgagtgaag tggcggccga aacggagctg
     6301 cagacgagcg aagggccaag acgcgtgagc gtcacggtgc aggccatcca ggaagatggc
     6361 gaacgcatgg gggcgctcgt gacgctgcgc gacctggact cgctggaaag catcaataca
     6421 cagcttcagg tgagcgaacg cctggcggcg ctggggcgca tcaccgcggg cgtcgcgcac
     6481 gaagtgaaga atccactgaa ttcgatgagg ttgtggctgg agaacttgaa ggagtcgctc
     6541 cccgcggacg ccgacggcgc gtcgcagcag gccgtgcagg tgctggacaa agaaatcgac
     6601 cggctggacg cggtggtgaa gcggtttctg gatttcacgc ggccgatgga tatccgcctg
     6661 gaagcgacgc agctcgcgga gcttttgaag gaagtgctgg aagtcgcaca acagcaattt
     6721 cagaaatcca acatccaagt ggcgcagttg ctgccgatcg atgtacccga ggtgtacgtg
     6781 gatcgcgcgc tgctcaagca ggcagtgctg aatctggtgc tgaatgccgc ggaggccatg
     6841 cccagcggcg ggcaattgcg gctagtgctg agccgccgcg gcgaaatggc ggaaattacg
     6901 gtgggcgaca ccggcaaagg cattccactg gaaaaccggc agaaaatttt ccaattgttt
     6961 ttcaccacgc gtccgggagg cagcggaatc ggacttgcga gtacgtttcg aattgtgcaa
     7021 cttctcaacg gttcgataga ctttacttcg gaggtcggcc ggggcacgac gttccgaatc
     7081 gaattgccaa tcgcggcctg aacatgacgg aaaacccagt ccggtcccta atggtgttgc
     7141 cgcgcggctt tctccagtcg ggcatatttc ttctctgttg tgccgcatgc gtgttgggtg
     7201 gatgcagtgc gaggaagcgg ccgagtattc cctgggtcac cgcggtacag gtgaggcctg
     7261 taacgcaagc gcacacagcg gcagcggaca gtgcttctga ggatttgctt ccggagctgc
     7321 ggcttgagtt gccgcctttt cccggccatc tgatcaccgt ccggacggca cctccgcggc
     7381 cacgcgtcag cacgccgccc tctgcgacca cggggaacga tgccgaaaag ttggatgcac
     7441 ccatgattgc cccgcagctt acggcacaag aatccgcggt tgcgcagcag cagacaaacc
     7501 agagtctggg catcgcggag aaaaatctgg agtccgcacg cggaaaaaat ctgaatgccg
     7561 cgcagtcgga cctggtctcc aaaatcagag gttttatcaa ggacgcgcgc gaagccgcgc
     7621 agatcacgga ttggagtcgc gctcgcagtc tagccaaaaa agcgcaggtc ctgtctgagg
     7681 aactcgccgg ttcgctgtag ttccccattc acatcccatg ttccttcgct gagaaggcac
     7741 tccgctcgtg caaatcgcgg ccatgaaaga agcacgcggc cagatgcgtt aagatgactt
     7801 catgaagaag ttcgatgtcg ccatcgcggg cggcggtgtg attggcggag ccatcgcgct
     7861 ggagcttgtg cgcgccggtt tgcgcgtggc ggtctttgac cgccagcaac cgggccagga
     7921 agcgtcttgg gccagcgccg gaatcctttc cccggcgccg gaaaatccca gaatgattgc
     7981 gatggtgccg ctgggaaagg cgagcctcgc gatttatccc gaatttgttg cgcaggtcga
     8041 ggaaatttcg gggaagagca ctggctttcg gccgaagggg acgctcgagg cgctgttctc
     8101 gcacgatacg aaagcggagc tcagcaccat tatcgcgctg catcacggcc tgggattgaa
     8161 ggcggagccg cttcgcgccg aggatgcgcg cgagctcgaa ccggcgctga gtgaagaagt
     8221 cgaagccgcg gtgctgcggc cggaggaagc ttcggttgac aaccgcttgc tgacggcggc
     8281 cattctggaa gcagcgcaac gcagcggggc cgagattttc cccggcaacg gcgcgaaagc
     8341 catctggcgc gaggggaatc gctgcgcggg attggttctg cagaatgaga aagtggaagc
     8401 caaatggacg atcatcgctg cgggctgctt ttccgcgaca atcgaaggga ttgcaccgta
     8461 cgcgccggtg cggcccgcaa aaggacagat ggctgcgctg cgcgccgatg atttgaagat
     8521 ggaccgcgtg ctctggtccg agaagattta tttggtgccg cggaacgacg ggcgaattgt
     8581 cgcgggggcc acggtcgagt atgccggttt cgacaagcgg acgaccgcgg gtggcatcga
     8641 gaaaattctt tcagccgcca tcgaccttgc gccgggcctc gccaatgcgc gcatcgaaga
     8701 aacatgggca ggcctgcggc ccgattctcc cgatcacctt cccatactcg gaccgaccga
     8761 tatcgatggc ctgctgatgg ccacgggaca cttccgcagc ggaattctgc ttacacccat
     8821 cacggcgtgt ctgattcgcg aatggattac agagcagcgc gttagcgtgg attgggaccg
     8881 cttcagcccg ttgcgatttc agtccgccgc gactgacgcg gcgcgtcacg cgcccgcctg
     8941 aaactctcta aacttggaaa atagaaaaat ggaaattgga aaaaaggaga ggagagcggc
     9001 ccccacccta gcatctggct agagtggtcc aacttctacc acccggcccg gttgaatgga
     9061 gagaagacct tcagcgcagg ctggcatgag cggtaataag tccttcaagc gatttgattt
     9121 tgcacggagg atcaggatgg aaactgttcg cccagccatg ttttgctggt acttgatatt
     9181 gcggtcgctt gtgagcagca cgtcccactg accttcagcg agcgcgagga gttcgccgtt
     9241 ctttttcgat ccatatccgg ctcttcgcgc cgtttggcac tcgtgaccca tagcggcgac
     9301 gctctctttt agctcgtctg gaaggcattc atcgataagg atcttcattt cccgaagcga
     9361 gccaaaagga gagttttggc ttcttctaac gcggcgatgg cttgctggcg gcttacgccc
     9421 gggtattgtt tcagaaactc atctagcgtt tctcccccct ccaaatagtc aagcaaggtc
     9481 tggaaaggga cgcgcgtgcc acggaaaacg ggttctccac tgtggatctc cgggtcttgg
     9541 acaattacac tgtctgagac aattgacatt atggcacccc tgcgaatatc ccgattatct
     9601 catcactctg cctagagtgc cgtgaaaaat gcaataggtt gggaatgaag ttcactctaa
     9661 acccgcacac gccccgaccg ggtcggggta aacgccaccg gcgtatgcgg cacccagaat
     9721 cctcccagaa tttgtcgtct ctgccacccg ccgatttcag cccgccgcga gtgacgccgc
     9781 gcggcacgcg cccgcctgaa gctctctaaa acttggaaaa tagaaaaatg gaaattagaa
     9841 aaaaggagag gagaacggcc ccaccgttca tcccgcgaat gcagtgggat ggggcggagg
     9901 tgtaaaaacc cacaaaggga aggccaccca aaggggtttt tgggaattaa ggcgcggcca
     9961 cccgcccaag tcggataaat gtcggaaaac gtcccgtctg tccctggccc cctggcccca
    10021 gtgtgcgaac ggagcggcgt cagtcggatt tgttgtactc agtgcaggct ccaaagatgt
    10081 tgagttttgg ctggtagaat ccgcagattt cggtgtggtg aggttggttg aatacgtctt
    10141 tgtacgaggc ggttgcgaag acgtagagca ctgcctcgcc gttggtcatc tggtcgacca
    10201 tagactgtag atatggggct tgtgaatctg tttgcatcgt tgaggtctgc cctgagggga
    10261 gggtgaacac actgttggtg ggtccgcgtt gcggatagga ggatgcgatg tcgcgggcgg
    10321 ttttagttgt ggtggataca ggagcgaagg aaaagaaaag gagaactcct acatctaggg
    10381 ccggcgtgcg tcccacgttg gcaatggtag ctatggggtg aactctctct cccgcagctg
    10441 gcggtttcaa cagggaagca gtgatgtgga cgtaggcgcg ctgggagacg tgcaacgctt
    10501 gcgatgcagt ggacgcactg gcttcgccgg ccttcgcact ccgttcggcc tgggcctcgg
    10561 acttcttggc agcgtccagt tgcatttgca atatcgtgtt cattgattcg gtggcgctct
    10621 gaagagaggt caaggtctta gcggttgcgg ccgagctgtt ttgcagattc gttaggatct
    10681 gctgttgttt gtcaaagttt tgggactgtt ggtggccgcc ccagaggctc aggaggattt
    10741 ctcctccgat gagaaggacg acgacaattt ctaatgcgaa tgtgatttgt tcgcgtcgtt
    10801 ggcgggaccg ggtacgttcc gtcagccagt ctctagcgtg ctcgacattc tggtcaaagc
    10861 taccggcgac ctgcatggca atgacggttt ctccgtaccg ctcgaaaatt tctcgttcag
    10921 cgggcgatat gttcgctttc cgagactttg gcgagggcgc ggctgcggcc ttgggcggcg
    10981 ggggcgacgg tggctggtct ggcgttttgt cggtgtcttc catgggttgg cgtgagttct
    11041 ttatatcaga ttggtgtcca gatgtggacg ccttcacgga ccggactgga tctggggggc
    11101 ggtggccgga gcggcgcacg gaccgcattc cggagggcgg atggtttttc agttgtcgag
    11161 gatgatggct gcgggaagat ggcccgcccg gaggtttgcg ggagtacgcc gcggaggtca
    11221 gcgcgcgtgc ttggcgggat gctaaaggct atagatcgcg ttcgcgattg tgaggacctc
    11281 gggccacgtg atggacggct ggaccttcat cgccatgatg actggattat ctgacctcca
    11341 cttcttgacc gcttccagca aggcttccgt tgcgttgcgg gtcaccgggt cgacagcgcc
    11401 cgtgttgccg gcattcac
//
LOCUS       DASYVO010000182         3177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_143809, whole genome shotgun sequence.
ACCESSION   DASYVO010000182 DASYVO010000000
VERSION     DASYVO010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143809"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VGR03_06135"
     CDS             complement(<1..573)
                     /locus_tag="VGR03_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEV1993889.1"
                     /translation="MLPGGHLLSVLIFFPAVGALALLMLRGDDHKFIRIIALIVSLAE
                     FLFSLLMIRAVPVGAAGYHLEEYLKWINQPPINYHLGVDGISLFLVILTTFLTPISVL
                     ASWKSIEHRVKEFFVMLLMLEVGVVGVFLSLDLFLFFLFWEVMLIPMAFLIGIWGHER
                     RVYAAVKFVLFTMAGSILMLVGIIWLYNATG"
     gene            complement(573..2558)
                     /gene="nuoL"
                     /locus_tag="VGR03_06140"
     CDS             complement(573..2558)
                     /gene="nuoL"
                     /locus_tag="VGR03_06140"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEV1993890.1"
                     /translation="MPTHPMLEYLWIVPLLPLLGAAVNGIFGAKWSNKIVNSVAIGST
                     GLSFLCALEAVREFFESGQVLFHKEFFTWIAAGNFRAGFDLQMDQLTVVMVLVVTGVG
                     WLIHIYATGYMAHEGGYYRFFSYLNLFMFFMLILVLAANYVLLFVGWEGVGLSSYLLI
                     GFYFLKKSASDAGKKAFIVNRIGDFGFMLGMFLLFKTFGTLDFVQLFAKAGPWPADAM
                     GQLGTFTIACLLLFMGACGKSAQLPLYVWLPDAMEGPTPVSALIHAATMVTAGVYVVA
                     RSHVLFTHAPTAMLVVAIVGCATAFFAATIGLVQTDIKRVLAYSTVSQLGYMFLACGV
                     GAFSAGIFHLMTHAFFKALLFLAAGSVIHAMGGEQDMNRMGGLSKKIRWTYLTMLTAT
                     LAIAGFPPFAGFFSKDTILLYSLLSEPGGLWLYAVGLLTALLTSFYMFRVIFLTFSGK
                     PRYDEHKVHVHESPWTMLGPLVVLALLSVCGGWLAVPSYFGGEDLFKAFLSPVLSDIE
                     PNVGSPAFPGADAPNEMVFAAIAVVVVLIGLLVAWWLYTKRPGKPAQFANSIKGTYRV
                     LLNKYYVDELYAAVVVKPLVWISTTVLWKAADVAGIDGAVNGIAEGTTAIGDGVRRTQ
                     SGNTRSYAVWVVVGALVVIAVIFFWPSGGKPVLGMVP"
     gene            complement(2558..2860)
                     /gene="nuoK"
                     /locus_tag="VGR03_06145"
     CDS             complement(2558..2860)
                     /gene="nuoK"
                     /locus_tag="VGR03_06145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEV1993891.1"
                     /translation="MVPTNYYVILSAILFGLGVFAFIFKRNIITIFMSIELMLNAVNL
                     AFVAFSQALGKLDGQVFVLFVIVVAAAEAAVGLGIIILIARNRQTLNVERVDLLKL"
     gene            complement(2862..>3177)
                     /locus_tag="VGR03_06150"
     CDS             complement(2862..>3177)
                     /locus_tag="VGR03_06150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEV1993892.1"
                     /translation="GAIMVLFVFVIMLLNAGIEEHTSISKMAGAPGLLLVVALAGFVA
                     ATIARSSDSVQTKLQTGEMSSTLGISNMIFKEFVYPFELTSFLILVAVLGATVLAQRE
                     KS"
BASE COUNT          824 a         1007 c          817 g          529 t
ORIGIN      
        1 gcccgtggcg ttgtagagcc agatgatgcc caccagcatc agaatcgatc ccgccatggt
       61 gaaaagcacg aacttcacag cggcgtacac gcggcgctcg tggccccaga tgcctatcag
      121 gaacgccatc ggaatcagca tcacttccca gaaaaggaag aagaggaaaa gatccagcga
      181 gagaaacacg ccgacaacgc cgacttccag catcagcagc atcacgaaaa attctttcac
      241 gcggtgctcg atgctcttcc acgaggccaa aacggagatg ggcgtcagga aagtcgttag
      301 gatgacgagg aacaaactga ttccatcaac gcccaggtgg taattgatcg gcggctggtt
      361 gatccacttg aggtattcct cgaggtgata cccggcggcg ccaacaggaa ctgcccggat
      421 catcaaaagc gagaacagaa attcagccag ggaaacgatc agcgcgatga tgcggatgaa
      481 tttgtgatcg tcgccgcgca gcatcaagag cgccagcgcc cctacggcag ggaagaagat
      541 cagcaccgag agcaaatgtc cgccgggcag catcagggca ccatcccgag aaccggtttt
      601 ccgccggacg gccagaagaa gatgaccgca atcaccacca gcgctccgac gaccacccac
      661 accgcatagc tgcgtgtgtt cccggattgc gtacggcgca cgccgtcgcc gatcgccgta
      721 gtcccttctg caatcccatt gacagccccg tcaattccgg ccacatcggc cgccttccag
      781 agcacagttg ttgaaatcca caccagcggc ttgaccacta ccgcggcgta cagttcgtcc
      841 acgtaatact tgttcaggag aaccctgtat gtgcccttaa tggaattcgc gaattgcgcg
      901 ggcttgccgg gtcgcttagt gtagagccac cacgctacca gcagcccaat caggaccacg
      961 actaccgcaa tggccgcaaa taccatctcg ttcggcgcat ctgcgccagg aaacgcggga
     1021 gaccccacgt ttggttcgat atcggagagc acagggctga ggaacgcctt gaaaaggtcc
     1081 tcgccgccga aatacgacgg taccgccagc cacccgccac atacagacaa caaagcgaga
     1141 accacgagcg ggcctagcat ggtccaaggg gattcgtgca cgtgaacttt atgctcgtca
     1201 tagcgcggct ttccggaaaa ggtcaggaaa atgacccgga acatgtagaa cgacgtcagg
     1261 agtgctgtca gcaggccaac ggcgtaaagc cacagtccgc cgggctcgct aaggagcgag
     1321 tagagcaaaa tcgtgtcctt gctgaaaaat cctgcaaacg gcggaaaacc agcgattgcc
     1381 agcgtcgccg tcagcatcgt cagataggtc caccgaattt ttttgctcag tccgcccatg
     1441 cggttcatgt cctgctcgcc gcccatggcg tgaatcacgc tgcccgccgc aaggaagaga
     1501 agcgccttga aaaacgcatg cgtcatcagg tggaaaatgc ccgcggagaa cgcgccaacg
     1561 ccgcaggcca agaacatata gccgagttgg ctcaccgttg agtacgccag cacgcgcttg
     1621 atgtccgttt gcaccaagcc gatggtcgcc gcaaaaaacg ccgtcgcgca ccccacgatg
     1681 gccaccacca gcatggccgt cggcgcgtgc gtgaagagaa cgtgcgaacg cgcaacgaca
     1741 tacactcccg cagtcaccat cgtcgccgcg tgaatcaacg cgctgaccgg cgtcggccct
     1801 tccatcgcgt ccggcagcca cacgtacagc ggcagttgcg cggattttcc gcacgcgccc
     1861 ataaaaagta gcaggcaggc gatcgtgaac gtcccaagct gtcccatcgc atccgcaggc
     1921 cacggaccgg ctttggcgaa gagctgcacg aaatccagcg tcccaaacgt cttgaacagc
     1981 aaaaacatcc ccagcatgaa tccaaagtca ccgatgcgat taacgatgaa cgctttcttg
     2041 ccggcgtcgg aagccgactt cttcaagaaa taaaatccga taagcaagta gcttgagagc
     2101 cccacgccct cccagccaac gaagagcagc acataattcg cggccagcac caggatcagc
     2161 atgaaaaaca tgaacaggtt aaggtacgaa aagaaccggt agtatccgcc ctcatgcgcc
     2221 atgtacccgg tcgcatagat gtgaatcagc cagcctactc cggtcaccac cagcaccatc
     2281 accaccgtga gctggtccat ctgcagatcg aagcccgcgc gaaaattgcc cgccgcaatc
     2341 cacgtgaaga attccttgtg gaagagcacc tgccccgact cgaaaaactc gcgcaccgct
     2401 tcgagcgcgc aaagaaacga cagtcccgtc gagccgatcg ccacactatt gacgatctta
     2461 ttcgaccact tcgcgccaaa aattccattc accgccgcgc ccagcagcgg cagcagcgga
     2521 acaatccaca ggtattcgag catcgggtgt gtcggcatta gagtttaagc aggtccacgc
     2581 gctcaacgtt cagtgtttgc cggttgcgcg caatcaaaat gataattccc agccccacgg
     2641 ccgcttccgc cgccgccacc acaatcacga acagcacaaa aacctgccca tccagtttgc
     2701 ccagcgcctg gctgaacgct acgaacgcca gattgaccgc attcagcatc aattcgatgg
     2761 acatgaaaat cgtaataatg ttgcgcttga aaataaacgc aaacacgccc agcccgaaca
     2821 ggatcgcgct cagtatcacg taataattcg tcggcaccat ctcaactctt ctcccgctgg
     2881 gccagcaccg tggcacccag gaccgccacg agaatcagga acgacgtcag ctcgaacgga
     2941 tagacgaact ccttgaagat catgtttgaa atcccgagcg tcgaagacat ttctccggtt
     3001 tgcagcttgg tttgcacgct atccgacgac cgcgcaatcg tggctgccac gaagcccgcc
     3061 aacgcgacca ccagcagcaa acccggcgct cccgccatct tcgaaatgct ggtgtgctct
     3121 tcgatgcccg cattcaacag catgattacg aacacgaaca gcaccatgat cgcgccg
//
LOCUS       DASYVO010000183         3252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_73840, whole genome shotgun sequence.
ACCESSION   DASYVO010000183 DASYVO010000000
VERSION     DASYVO010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73840"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            558..1700
                     /locus_tag="VGR03_06155"
     CDS             558..1700
                     /locus_tag="VGR03_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV1993893.1"
                     /translation="MGLSSGGVLLAGFDKILLPATAGPRREDPFSGGQQLGVVEFLHE
                     GRAGLETVIGNELDGRMYTDLASLSLQDAATPTEKFYIRTRASKLMPDAKSWQVRVEG
                     FGEKPRTLSLEYLKRAAKPLGRHLMECAGNVPLVRFGMISVASWAGVPLAEILDGTGA
                     RPQAPRVLITGFDQYATKSVTSVPGASWVFSLEELRSARAFLATEMNQLPLTSDHGAP
                     GRLVVPGWYGCTCIKWVTRIALVNETAEATSQMQEYAGRTHQHGLPALARDFQPATID
                     PAAMAIRVENWLVEGKIKYRVVGIAWGGSTPVKTLMIRFNPEEDYVPVDRFAQTKNDP
                     WTFWTHAWSPREPGTYSIRLAIKDPAVRTRRLDSGYYVRTVEILEV"
     gene            1750..>3252
                     /locus_tag="VGR03_06160"
     CDS             1750..>3252
                     /locus_tag="VGR03_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993894.1"
                     /translation="MAGWVRSLLFVCVIPGAAARAQHSPQEGHSHTGPVPLEILQRPV
                     TLRPGIGELHEKVSTHSPEAQSFYDQGLAYLHSYVWIEAARSFHQALRLDPNLAMAYL
                     GLTDAFIGLHDVATARATFERARALEKGMSERERMWLLIRESELEFLEDSENPNGYAA
                     YRKSINEALKAYPNDPWLWIQRGLADEGNPFTHGQAGGVDALAFYRTALALAPGNLPA
                     HHYCAHAYENIGRSKDALEESALYARMAPAIPHAHHMHGHSLMRAGRTEEAIGEFLKT
                     KELEENYYRSEKIPARFDWHHAHNLTLLAMSYQSLGQIKSAEALFQETFSSPAYTDFL
                     EYNRKSWPEFLLSRERFQEALLAARELTKSEWPLARLAGHTLAGQALLRMGSVTESKE
                     ELTMAERETERLPARAIAALPYPAALRADILLQEKNTTEGEPLAMDVAKSIQAMPGPD
                     AWIAALFELESIAQSARNAGEWELASFIARQMILHNPNYAGGHFAYGLAAE"
BASE COUNT          718 a          896 c         1005 g          633 t
ORIGIN      
        1 ggggtccgta acggtaccgg tgatgacgcc ctgcacagtt tgtgaccacg ccgctccgct
       61 ccccgcaaga atcaggagga aagctgcaaa caagacaact agagttcgca ctcgcacact
      121 acacccccta tttttgaaag cttttgggcg cactttcaga ggcaccccaa gaatcatgtt
      181 cgcgttcaga agtatgtgtt ccaccacgtc tggatcgccg ccgagggcag cgaaacaggg
      241 ccattgagac gtattcaatt cctgtgagag cgatttgtca agggaaattt agaaaaacga
      301 gagataaact ttagctaaaa caaatcgatt tctcaccggg gaagagcgcc cggttagaaa
      361 aacagcatcg aagcagggcg tcgcctctgg cccgtccgcg aacttcacag ccccgacgag
      421 aagaccgagg acttttcatc aggtacaatg ctggaccgga atggctcaag gctcggcacg
      481 accgcgggct gaagcgaaag tgacgagacc gggcagagag tgtgatgatg cgtgtgacgc
      541 ggagagaatt tgcgaggatg ggtctgagtt ccggcggtgt cctgcttgcc ggattcgaca
      601 aaatactcct gcctgcaaca gccggccctc gacgggagga cccgttttcc gggggccagc
      661 aactcggcgt ggtggaattc ctccatgaag gaagagcggg gctggaaaca gtgatcggaa
      721 atgaactcga cggccgcatg tacaccgatt tggcgagcct ctcgttgcaa gacgcggcga
      781 cgccgacgga aaagttttat atccggacgc gagcttcgaa gctcatgccg gatgccaagt
      841 cgtggcaggt gcgagtcgag ggatttgggg aaaagccgcg aactctcagc cttgaatact
      901 tgaagagagc agcgaagcca ttgggacggc acttgatgga gtgcgcgggc aacgtcccgc
      961 tggtgcgttt cgggatgatt agcgttgcca gttgggcggg cgtgccgctg gcggaaattc
     1021 tggacggtac tggtgcgagg ccccaagcac cgcgcgtgtt gatcactggc ttcgatcagt
     1081 acgctacaaa gtcggtgacg tccgtccccg gcgcgagctg ggttttttct ttggaagaac
     1141 ttagaagcgc gagagccttc ttggctaccg aaatgaacca attacctctg acatccgacc
     1201 acggggcgcc ggggcgcctc gtggtaccgg ggtggtatgg gtgcacctgc attaaatggg
     1261 tgacccgcat tgccttggta aacgaaaccg cggaggcaac ttcgcagatg caggagtacg
     1321 cgggaagaac tcaccagcat ggtctgccgg cactggcgcg agattttcag ccggcaacga
     1381 tcgatccagc ggccatggct attcgagtgg agaattggct cgtcgaagga aagatcaaat
     1441 accgagtggt ggggatcgcg tggggcggaa gtactccggt caagaccctg atgatccgtt
     1501 tcaatccgga ggaagattac gttcctgtgg atcgctttgc gcaaacgaag aatgatccct
     1561 ggacgttttg gacgcacgcg tggtcgccca gggagccggg gacgtattcg attcgactgg
     1621 caattaaaga tccggcggtt cggacccggc ggctggattc gggatattat gtgcgcacgg
     1681 tcgagattct ggaagtatga gcaggttttc tgcgtacgct ggagacatcg aggtgagtgg
     1741 gtgaggcgag tggctggctg ggtccgttca ctgttattcg tgtgcgtcat cccgggcgcc
     1801 gcggcgcggg cgcaacactc gccgcaggag gggcactcgc atacaggccc ggtcccgctg
     1861 gaaattctgc agcgccccgt gacgctccgc ccggggattg gtgaactcca cgaaaaagtg
     1921 tccacgcatt cgcccgaggc gcaatccttc tacgatcagg ggctcgccta cctgcattcc
     1981 tacgtttgga tcgaagccgc gcgttccttt caccaagctt tgcgcctgga tccaaacctg
     2041 gccatggcct acctgggcct gaccgatgcg ttcatcggcc tgcatgacgt tgcgaccgcg
     2101 cgggccacgt tcgagcgcgc aagagcattg gaaaaaggaa tgagtgaacg cgagcggatg
     2161 tggcttctca tccgcgaaag cgaactcgaa tttctggagg acagcgagaa tcccaatggc
     2221 tacgccgcct accgcaagtc gatcaacgag gcgctgaaag cgtacccaaa cgacccctgg
     2281 ctgtggatcc agcgcggatt ggcggatgaa ggcaatccct tcacacatgg gcaagccggc
     2341 ggggtggacg ctctcgcatt ttacagaact gccctggcgc ttgcgccggg gaatcttccc
     2401 gctcatcact attgtgctca cgcgtacgaa aacatagggc gatcgaagga cgcgctcgaa
     2461 gagagcgccc tctatgcgcg catggctccc gcgattccgc acgcgcatca catgcacggt
     2521 cattcgctca tgcgagccgg ccgcaccgag gaagcaatcg gcgagtttct caagacgaaa
     2581 gagctcgaag aaaattacta ccggtcggag aagattccgg cgcgcttcga ttggcaccac
     2641 gcgcacaatc tcactttgct ggcgatgagt taccagtcgc tgggacaaat caaatccgcc
     2701 gaggctctgt ttcaggagac tttctcttcg cctgcctata cagattttct ggagtacaac
     2761 cgcaagtcct ggccggagtt tctgttgagc cgcgagcgat ttcaagaggc tctgctggca
     2821 gcgcgcgaat tgacaaaaag tgagtggccg ctggcgcgcc ttgccggtca cactttggct
     2881 gggcaagcac tcctcaggat gggttcggtt acggaatcca aggaggaact gacgatggca
     2941 gagagggaaa ccgaacggct tcccgccaga gcgatcgccg cccttcccta ccccgcggcg
     3001 cttcgggccg acattttgtt gcaggaaaag aacacgactg agggtgaacc gctcgctatg
     3061 gatgtggcta aatccatcca agcgatgccc ggccccgacg catggattgc tgcgctattc
     3121 gagcttgaat ccatcgcgca gagcgctcgc aacgcgggcg aatgggaact ggccagcttc
     3181 atcgcccggc agatgattct gcacaacccg aattatgcgg gtggccactt tgcctatggc
     3241 ctcgcagcag ag
//
LOCUS       DASYVO010000184         1811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_214544, whole genome shotgun sequence.
ACCESSION   DASYVO010000184 DASYVO010000000
VERSION     DASYVO010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214544"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..891)
                     /gene="xerD"
                     /locus_tag="VGR03_06165"
                     /pseudo
     CDS             complement(16..891)
                     /gene="xerD"
                     /locus_tag="VGR03_06165"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
     gene            complement(909..>1811)
                     /locus_tag="VGR03_06170"
     CDS             complement(909..>1811)
                     /locus_tag="VGR03_06170"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEV1993895.1"
                     /translation="LMLPRLFGIGIGQINLLIDTRFATAARMPPGSLAALYVADRVME
                     LVLGGYAIAVATAILPMMSHQAAAKDFESLKKTLSFSVRIVAFITIPAALGLMILREP
                     IIRVLFQHGQFVAESTRLTARALLYYAVGLPALATVKLIVPAFYSTRDTKTPVIIASI
                     SLVLNIVLNIVFLQFFFKRVQNGGPALATALATFFDFFALFIIFRLRYGPIGTMDIFR
                     SFSKISLCAGIMGVACWFGNYYTGFALHSRFLVQLLVFAGLIIGATALYLALAWLFRC
                     HEIEEVYGIATRRTVGAGDGYVGS"
BASE COUNT          423 a          503 c          574 g          311 t
ORIGIN      
        1 ccctggcctg ccgcgttacg cacgcgggtg atgcgctttg taaatctgct tcaaccgctc
       61 ctcgacgaca tgggtgtaaa tctgggtggt ggaaatgtcg gcgtggccaa gcatgagctg
      121 cacggaccgg aggtccgctc cgcgctcgag caagtgcgtg gcaaagctgt gccggagcat
      181 gtgcggggta agcgccatgc gcatacccgc ccgccgtcca taggccgaaa gtatcttcca
      241 cacgccgacg cggctcaatt gcgcgccgcg gcggttcacg aacaacgtcg gactgcttac
      301 cactttcccg agcagcttcg gcctggcgtc gcggagatac ttttcgacga tggcgagggc
      361 tttcttgcca acgggaacaa ttcgttcctt gtcgcctttg ccgatgcaac gcacgcagcc
      421 aaccttggca tcgagatcgg taatgcgcag cccagtgagc tccgatacac gcaggccggt
      481 ggagtacagc acctccaaca tggcgcggtc ccgcaaaccg atgggagttt tcacatcggg
      541 ctgggcgagc acgcgctcga cttcctcgag gcggagatag cccggcaagg aacggtggat
      601 cttgggcgat tcgagattca ccgaagggtc ttctgaaatc tggtcctgga tctgcgcgaa
      661 acggaaaaag ttgcgcaggg taaccaggtg acgggccaca gtcttgctct cgagcttctg
      721 acgataaagc cccgcgagga agtccaccaa atcgtcgcgg ctgacggctt cgaggctaag
      781 cttgcgtttg cgggcaaatt catcgaattt tacgagatcg tggcgatacg cggagaccgt
      841 gttggaggac agccccttct ctacccgcac gtgcgtcaag aatgacgaaa tggttgccgt
      901 aatttccatc agctccccac gtatccatca ccggcgccaa cagtgcgccg cgtagcaatt
      961 ccatagactt cctcgatttc atggcagcgg aagagccaag cgagggcgag ataaagggcg
     1021 gtcgctccta tgatcaggcc ggcaaaaacg agaagctgca cgaggaagcg cgagtgcagc
     1081 gcgaacccgg tgtagtaatt cccgaaccag caggcgacgc ccatgatgcc ggcgcacaag
     1141 gaaatcttcg aaaaggaacg gaagatatcc atggttccta tcgggccata gcgaaggcgg
     1201 aaaataataa agagcgcgaa gaaatcgaag aaggtagcca gggccgtggc gagcgccgga
     1261 ccgccgttct ggacgcgctt gaagaaaaat tgcaaaaaga cgatgttcag aacgatattc
     1321 aggacgagag agatggaagc gataatgacc ggcgtcttgg tatcccgcgt ggaatagaac
     1381 gccggaacaa tcagcttcac cgtggccaag gccggaagtc cgacggcgta atagagaagc
     1441 gcgcgggccg tcaagcgcgt ggattccgcc acgaactggc cgtgctggaa gagcacgcgg
     1501 atgatcggtt cgcgcaggat catcaggccg agagccgcgg gaatggtgat gaatgccacg
     1561 atgcgaacgg aaaacgagag cgttttcttg agcgactcaa aatccttggc ggccgcctgg
     1621 tgagacatca tgggcagaat ggccgtggcg acggcaatgg cgtagccgcc cagcaccagc
     1681 tccatgacgc ggtcggcgac gtaaagcgcg gcgagactcc cggggggcat gcgagcggcg
     1741 gtggcgaacc gcgtgtcgat cagcagattg atctggccga tgccgatccc gaaaagccgc
     1801 gggagcatca a
//
LOCUS       DASYVO010000185        10688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_117286, whole genome shotgun sequence.
ACCESSION   DASYVO010000185 DASYVO010000000
VERSION     DASYVO010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 10688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10688
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117286"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VGR03_06175"
     CDS             complement(<1..809)
                     /locus_tag="VGR03_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993896.1"
                     /translation="MHATTQAKGQSKRHAMLHRCVSAAVMLFASMMLVSCKPPNYPTK
                     PIELKYYSAGPWAVTVAIGAECCDSSGDKLDLYYPTNLGQNGFQHPILTWGNGTNSLS
                     GNYTFFLKHMASWGFVIIAAQDKNSGVGQTILDGANFLIAANSNPVSIFFHKLNVSQV
                     GSFGHSQGATGAINALIKSAGTIKTVVPIELPGQQFCANPLNCADTSHVTQGSIFMVD
                     GSLDIPISPPTQPASATGLQSIAAYYSAVPAGVVKVKGTLIGPTHCDVQGVP"
     gene            1098..3554
                     /locus_tag="VGR03_06180"
     CDS             1098..3554
                     /locus_tag="VGR03_06180"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEV1993897.1"
                     /translation="MASYSQFGLRIRLLPFLGAVLLFPTLRADTAAQEPSPQVSAKSA
                     PAQWITAPGAPAKDLTMLHFRKEIELEKVPEHFLVDVSADNRFLLMVNQERVGSGPAR
                     GDLAHWRYEVYDLAPHLRAGKNVLAATVWNLGTFAPIAQISNRTGFLVRGESQLEQMA
                     DTNDSWLAEQEPGFQVAAVSMGELRGYFAADPIEKIDGRLFDWTWDSQTTASPHWQKA
                     QPLGPAANGGLGDTPNNWQLVRDPLPAMESRETSVGRIVRASGLATIATSGGFPDAPL
                     AIAPNQKVTLLLDSAELTTAFTNLVVSGGKDASIRVTYAEALKDAKGEKGDRNAIAGK
                     QIIGVYDEYIADGAENRTFSSLVWRTWRYLQLDIETHDQPLQLLRLSSWFTAFPFQQR
                     ARFVSDDPALSSIWEVGWKTARLDAHETYMDTPYWEQLQYVGDTRIQALISYVVAGDD
                     RLAKQAIEAFDDSRIPDGITLSRYPAHHFQAIPPFSLLWVGMLHDFAMYRSDPAFVRA
                     HLPGTRTVLDWFVRHQQENGLLGRLPWWNFIDWTNGFRDGVPPQDEHGNSASITLHFV
                     MALREAAELESAYGDKQRAEQYLLAATKAAQGVQRLCWNEKHGLIADTPGQELYSQHA
                     NLLAVLLDVIPMQRQREVMSKVLSVSDPVFKMGGDLPPMEKASYYYRFYLARALAHAG
                     MGDRYLELLGPWKEMLALGLTTWAETPEPTRSDSHAWSAHPNYDLLTIVAGIRPATLG
                     FEKVVVEPHLGNLQNVTVTFPWKESEIQVGYHLERGTWTVRVNLPPSLQGEFVWQGKR
                     YPLHTGPQVLTLPPVVSPAK"
     gene            3898..4743
                     /locus_tag="VGR03_06185"
     CDS             3898..4743
                     /locus_tag="VGR03_06185"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEV1993898.1"
                     /translation="MELSATQPARPWDRPFWKPVHGKEGVFTYLVLIHVLAVIGLILF
                     PLPSLKVVGMTLLSIAFGGLGTTVCYHRMLAHKTVKTNRFIEQFLIFWAVFNGSGHPA
                     SWVAYHRLHHAVTDTPEDISSPKQGGFWWAHLRWLYQTQPADKQRWAPELTGGVYRIW
                     GWAETPVIVFSICIGLILGFGWMGFFWMGAIRLVYSLHMQCLVNSLTHLNHVKEGDSS
                     MNVWWLGPFQLTAWGENWHQNHHTHAGSARLGLRWYQTDIGWYFIWMLEAVGLAHSVK
                     RPRAS"
     gene            4816..5076
                     /locus_tag="VGR03_06190"
     CDS             4816..5076
                     /locus_tag="VGR03_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993899.1"
                     /translation="MYAQKFNVHVVIRGATRVCPLDWLDQFCMRNFTNSADFDDTLPV
                     ADGKVEASFCLTPERFAEGLAAWLTQRGKGEGQPVTVRVTRV"
     gene            complement(5137..6021)
                     /locus_tag="VGR03_06195"
     CDS             complement(5137..6021)
                     /locus_tag="VGR03_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEV1993900.1"
                     /translation="MAEEGKVSGTLGWLRTAKDKFRAFAYGEVSEPRERPRVGLALAG
                     GFARGIAHIGVLRVLREAGIPIDCVAGTSVGALIGASYCAGASLEEMEKIGGDTSFTD
                     FGRWTPSWLGLATNQRMEKYFARFTPVKTFEELQTPLAIATTDINAGVSVYYSHGSLA
                     PVIRASCAYPGLFVPIQYDGRTLVDGFLTAPVPVEGALLLGADIVIAVYLEAGSLEQP
                     RTFTDVLSRAFTIIQRHADLTWRTQADVIIEPDVKPFVWDDFTKTPEMVAAGEAAALA
                     ALPQIRAALRGEKRDSAA"
     gene            6139..8562
                     /locus_tag="VGR03_06200"
     CDS             6139..8562
                     /locus_tag="VGR03_06200"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEV1993901.1"
                     /translation="MIRRIEKAVVLGAGTMGSRIAAHFANAGLPCILLDIVPPNLPAD
                     APAGERNKIVRAGLDAAKKSRPAAFFTAALAEKIAIGNFDDDLARCAEADWIIEVVAE
                     NLEIKRKLLARVAQFRKPGAIVTTNTSGLPVHLIAEGMSEEFQQHWAGTHFFNPPRYL
                     KLVEVIPGPKTSSDVVESLSGFCDRRLGKGVVVAKDSPNFIANRIGTFSMLNALRLMG
                     TLGMTVEEVDACTGPAVGWPKSATFRTADIVGLDVLVHVVKNIYETAPNDESRESYKV
                     PALVEEMAKRGWLGDKTGQGFYKKVRGEGEKEILTLDVNTMEYRARQKAKFASLEMGK
                     AIEDTRERLRALVGPVLEGQKGDKAQQFLWGALSEMCLYAARRVPEISDNVVDVDRAL
                     RWGFAWELGPFQIMDAIGVKAFAGQVQKEGRALPAVIEKMLASGRKGFYESEKGTTTV
                     FDIGIGAAKKVEEPKGVIILKSLKDAGREVERNSGASLIDLGDGVVCCEFHAKMNAIG
                     ADLIAMLHKGLKRLETDFDAMVIANQAVNFSVGANLMLVLVGAQEQEWDELHMAVKQF
                     QNINLAIKYAPKPVVAAPQGMALGGGCEVSLHAAKIQAAAEAYIGLVEAGVGLIPGGG
                     GTKEMLIRANEHAAGGEDLDLFHALKPVFEAIAMAKVGTSAEECRDLGYLRREDGVSM
                     NRDRLVGDAKEAALALVRGGFKPLAATWQEGAQTTQIKVLGEQFLAGAKLAIHMMLRG
                     GYASEYDAHVGRKLANILAGGALTSPQLVSEQYVLDLEREAFVSLCGEKKTQERIAHT
                     LKTGKPLRN"
     gene            8649..9056
                     /locus_tag="VGR03_06205"
     CDS             8649..9056
                     /locus_tag="VGR03_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1993902.1"
                     /translation="MQKLLAPREAANILGISYPTLKQWIYHGKIKSMKTPGGHHRVPE
                     SEIDRLFPKKLARGDFETRRGNFRKISGRNQLIGRVVAIKFSGLLAQVTLAIGEQHIT
                     SIITADAAKELRLKPGERAAALIKSTEVMILRV"
     gene            9167..9904
                     /gene="modA"
                     /locus_tag="VGR03_06210"
     CDS             9167..9904
                     /gene="modA"
                     /locus_tag="VGR03_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1993903.1"
                     /translation="MRAQGKEIRVAAAADLKFAMGELSEQFEKHTGTKVNVTYGSSGN
                     FFLQMQNGAPFDLFFSADIEYPRKLEAAGLAEPGTFYEYAIGRIVIWTPADAKVDATK
                     QGWKLLLDASVEKIAIANPEHAPYGRAAVTALQKAGIYESIKAKLVYGENISQAAQFV
                     QSGNAQAGIMALSLAVSPAMCDGKRWEIPAEMHPALEQGAIILKDAKNKDAARAFLEF
                     VKSAAGRATLAKYGFEFPEKMSKEEKR"
     gene            9953..10621
                     /gene="modB"
                     /locus_tag="VGR03_06215"
     CDS             9953..10621
                     /gene="modB"
                     /locus_tag="VGR03_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEV1993904.1"
                     /translation="MEALWLSMKLAGCVAAILLVIGMPVAYWLAYSSWRGKFLLESLV
                     ALPLVLPPTVLGFYALVAMGPRGPLGKLWIVLFGHGLAFTFGGLILASVLYSFPFAVQ
                     PLVASFEGVDRKLLDASAVLGAGGLRTFWRVILPLSLPGVVTAAVLSFAHTLGEFGVV
                     LMVGGNLAGVTRTVSIDIYDRVQSLEYGVANRMALVLLAISFAVLSVVYAVNRRVWTP
                     WAAK"
BASE COUNT         2234 a         3000 c         3251 g         2203 t
ORIGIN      
        1 ggcacgcctt gcacgtcgca gtgcgtcggg ccgatcagcg tgcctttcac tttaaccacg
       61 cccgcgggca ccgcgctgta atacgcggcg atggattgca gccccgtggc tgatgccggc
      121 tgcgtcggcg gagaaatcgg aatgtccaga gagccgtcca ccatgaagat cgagccctgc
      181 gtgacgtgtg atgtgtccgc gcaattcagc gggttggcgc agaactgctg cccgggcagc
      241 tcgatgggca cgacggtttt aatcgtgccg gcggatttga tcagggcgtt gatcgctccg
      301 gtcgcgcctt gcgaatgtcc gaacgaaccc acctggctga cgttcagctt gtgaaaaaag
      361 atgctgacgg ggttgctgtt cgcggcgatc aggaaattcg cgccgtccag gatggtttgc
      421 cccactccgg agttcttgtc ctgtgcggcg atgatgacga atccccagga cgccatgtgc
      481 ttcaggaaaa acgtgtagtt gccggagagc gaattggtgc cattgcccca ggtgagaatc
      541 gggtgctgga atccgttctg ccccagattg gtggggtaat agagatcgag tttgtcgccg
      601 gaggagtcgc agcactccgc gccgatcgcc accgtgacgg cccagggtcc cgcgctgtag
      661 tacttcaact cgatgggttt cgtcggatag ttcggcggct tgcaggagac cagcatcatg
      721 ctggcaaaaa gcatcaccgc ggcagaaacg caccggtgga gcatcgcatg cctctttgat
      781 tgtccctttg cttgcgtcgt cgcgtgcatt ctggcctccg gtcagcccgt cagagttctc
      841 ttctggcagc agtgagagca tgcttgagct ggtcagcctg cagggctgcc ctaggggata
      901 gtgcccctga agagtatttc gttgcacgcc atggtgaaca ccgcccactc cgcgttccgc
      961 tgccagccgt cgttccacat atttggtagc cgcggaggcc agcaaaaaat tcatcgctta
     1021 cggctccgca gtagactttc tctgtgaacc tgcgacagaa tctccctccg gacatatcga
     1081 acccaatgag caatcaagtg gcaagctact ctcaattcgg acttcgcatc cgcctcctgc
     1141 cgttcctcgg cgccgtgctg cttttcccca cactgagagc tgataccgcc gcacaagaac
     1201 cttcgcccca agtgtcggcg aaatcggctc ccgctcaatg gatcaccgca cccggagcgc
     1261 ccgccaaaga cctgaccatg cttcacttcc gcaaggaaat cgagctggaa aaagtccctg
     1321 agcatttcct cgttgacgtg tcggcggaca atcgcttcct gctgatggtg aaccaggaac
     1381 gcgttggctc cggccccgcc cggggagatt tggctcattg gagatacgaa gtctacgacc
     1441 tcgccccgca cctccgcgcc ggcaagaacg tgcttgcggc cacggtttgg aatcttggaa
     1501 cgttcgcgcc catcgctcag atcagcaatc ggaccggatt tcttgttcgc ggagaaagcc
     1561 agcttgaaca aatggccgat acgaacgact catggttggc cgaacaagag cctggctttc
     1621 aggtagccgc agtctcgatg ggcgaattgc gcggatattt cgcagcggat cccatcgaga
     1681 aaatcgatgg acgcctgttt gactggacct gggactccca aacaaccgcc tcgcctcact
     1741 ggcagaaagc tcagcccctg ggacccgccg ccaatggggg acttggagac acacccaaca
     1801 attggcagtt ggttcgcgat cctctgccgg cgatggaatc gcgagagact tctgtcgggc
     1861 gaatcgtcag agcgtcgggc ctcgccacaa tcgccacaag cggaggattt cccgatgcgc
     1921 ccttggccat cgcgccaaat caaaaagtga ccctgcttct cgacagcgcc gagttgacga
     1981 ccgcatttac gaacttggtt gtcagtggcg gaaaggacgc cagcatccgc gtgacgtatg
     2041 ccgaagcgct caaagacgcc aagggagaaa agggcgaccg caatgcaatc gcaggaaagc
     2101 aaatcatcgg tgtttatgac gagtacattg cggacggcgc ggagaacagg actttctctt
     2161 cgctcgtctg gagaacgtgg cgatatctcc agctcgacat tgaaacgcac gatcagcctt
     2221 tgcagcttct tcggctgtct tcctggttca ccgcgtttcc ctttcagcag cgcgctcgct
     2281 ttgtctccga tgatccggca ttgtcctcca tctgggaggt cggctggaag acggcgcgcc
     2341 tcgatgcgca tgagacctac atggacactc cctattggga gcagttgcaa tacgtcggtg
     2401 acacgcgaat acaagcgtta atctcctacg tcgtcgcggg agacgaccgt ctcgcgaagc
     2461 aagccatcga agcgttcgac gattcgcgca ttcccgatgg catcactctc agccgctatc
     2521 ccgcccatca ttttcaggcg attccgccgt tctctctcct atgggttggc atgctgcatg
     2581 attttgcaat gtatcgctcc gaccctgctt tcgtccgcgc gcatctgccc ggcacgcgca
     2641 ccgtcctcga ttggtttgtc cgccatcagc aggaaaacgg tctgcttggc cggctgcctt
     2701 ggtggaactt tatcgattgg accaatggct ttcgcgatgg cgtcccgcct caggatgagc
     2761 atggaaactc cgcctcgatc actctgcatt tcgtgatggc gcttcgtgaa gccgccgaac
     2821 tggaatctgc ctatggcgac aagcagagag ctgagcagta tctcctggcc gcaacaaagg
     2881 cggcgcaagg cgttcagcgc ctttgctgga atgaaaagca tggcctgatt gcggacactc
     2941 ccggccagga actctacagc cagcacgcca acttgttggc ggttcttctg gacgtcatcc
     3001 ccatgcagcg ccaacgcgaa gtcatgtcca aggtcctctc ggtcagtgac ccagtattca
     3061 aaatgggcgg ggacttgccg cccatggaaa aggcctccta ctattaccgc ttttatctcg
     3121 cgcgtgccct cgcgcacgcc ggcatgggcg accgctatct tgaactcctg ggtccatgga
     3181 aggaaatgct cgctctcggt cttaccactt gggccgagac ccccgaaccg acgcggtccg
     3241 attcgcacgc ctggagcgct catccaaatt atgacttgct cacgattgtg gctggaattc
     3301 gccccgccac tctcggattt gagaaagttg ttgtcgagcc gcatctcggc aacttgcaaa
     3361 acgtcactgt cacttttccc tggaaagaat ccgagatcca ggtcggctac catcttgagc
     3421 gcggcacgtg gaccgtccgc gttaatcttc cccccagcct ccaaggcgaa ttcgtctggc
     3481 agggtaaacg ctatccgctc cacactggtc cgcaggtgct cacgctcccg cccgtggtct
     3541 cccccgccaa gtaggacaaa catctcgtgt agcgggagag ctcgaaggtt gacaacaaac
     3601 acgaagagag atttcatcgc tcacaaaaag cgtgagcgat ggcgcagagg tctcagattc
     3661 gttcggaatg agggcagaag ctaaggcaag agagaagccc cgggtgtaaa agcccgaggg
     3721 ttacagtgag aacctcagag gttagaatcc cctgagctac atgctattca ccttttgtgt
     3781 tcaactttgt acagttttgc ggcgcagcgc accggaaaat ggtatggtta tgactgcctt
     3841 atacgttctg cagatcaggc acacgcactc tcagcatcgc agatcgccgg agattacatg
     3901 gaactaagcg ccacgcaacc agcacgcccc tgggaccggc ccttttggaa gcccgttcac
     3961 ggcaaagaag gcgtcttcac ttatctggtc ctcattcacg ttcttgcggt catcgggctg
     4021 atcctcttcc cgctgccgag tctcaaggtt gtgggaatga ctttgctatc tatcgctttt
     4081 ggcggtctcg gcacgaccgt ctgctatcac cgcatgctcg cccacaagac ggtcaagacc
     4141 aaccgcttca tcgagcaatt cctgattttc tgggccgttt tcaacggctc cggtcatccc
     4201 gcaagttggg tcgcgtatca tcgccttcac cacgccgtca ccgacacacc cgaggacatc
     4261 tccagtccca agcagggtgg cttctggtgg gcgcatcttc gctggctcta tcaaacccag
     4321 cccgccgaca aacagcgctg ggctcccgaa ctcaccggcg gcgtctaccg gatttgggga
     4381 tgggccgaaa cccccgtcat tgtcttctcg atctgcatcg gcctgattct cggcttcggc
     4441 tggatgggtt tcttctggat gggcgcgatt cgcctggtct actcgctgca catgcagtgt
     4501 ttggtgaaca gcctcacgca tctcaaccat gtcaaagaag gtgattccag catgaacgtc
     4561 tggtggctcg gtccgttcca attgaccgcg tggggcgaga actggcacca gaaccatcac
     4621 acgcacgccg gctccgcgcg ccttggcctt cgctggtatc aaaccgacat cggctggtat
     4681 ttcatctgga tgctcgaagc cgtcggtctg gcccactcgg tcaagcgccc tcgcgcttcg
     4741 tagctggcgg cgagccgacg agcccgcttt gtcctgaact gtaagggcgg ggcttaggcc
     4801 cgcgtcctgc gcttcatgta cgcacaaaaa ttcaatgtcc acgtggtgat tcgcggcgca
     4861 acccgcgtgt gcccgctcga ttggctcgat caattctgca tgcgcaattt caccaactcc
     4921 gccgactttg acgacacgct tcccgtcgcc gatggcaagg ttgaagccag cttctgcctc
     4981 actcccgaac gcttcgccga aggtcttgcc gcgtggctca cgcagcgcgg gaaaggcgaa
     5041 ggccagcccg tgacagtacg cgtcacgcgc gtatagttcc ccctgagtta ttcgaagaga
     5101 gtttttcgaa gattcgtccg cggataataa attgtgctat gcggcggagt cgcgcttctc
     5161 cccgcggagg gcggcgcgaa tctgcggcag cgccgccagc gccgcagctt cgccggccgc
     5221 taccatttcg ggcgtcttgg tgaaatcgtc ccacacgaag ggttttacat ctggctcgat
     5281 gatgacgtcc gcctgcgtgc gccatgtcag atcagcgtgc cgttgaataa tcgtgaacgc
     5341 ccggctcaga acatccgtga acgtgcgcgg ctgctcaagg ctgcccgcct caagatacac
     5401 ggcgatgacg atgtctgcgc cgaggagcag cgcgccctcc accggcaccg gagcggtaag
     5461 gaatccatcg acgagcgtcc gtccgtcgta ctggatgggc acgaatagcc cggggtaggc
     5521 gcacgaagcg cgaatcacgg gcgcaagcga gccatgcgag taatacacgg agacacccgc
     5581 gttgatgtcc gttgtggcga tggccagtgg cgtctgcagt tcttcgaatg ttttcacggg
     5641 agtaaagcgc gcgaaatatt tttccatgcg ctggttggtg gccaggccga gccacgaagg
     5701 tgtccagcgg ccgaaatcgg tgaagctcgt gtccccgcca attttttcca tctcttcgag
     5761 cgatgccccg gcacagtaac tcgcgccgat tagtgcgccc acgcttgtgc ctgcgacgca
     5821 gtcgatgggg ataccggctt cgcgcaaaac gcgcaggact ccgatatgcg caatgccacg
     5881 cgcaaagccg cctgcaaggg ccaagccgac gcgtgggcgt tcccttggtt cgctcacctc
     5941 gccgtacgcg aaggcgcgaa acttgtcctt tgccgttcgc aaccagccga gagtgccgga
     6001 gactttgcct tcttccgcca tttctctatt ttagtcctat cgccgcgaac cagcctgtcg
     6061 tttgacccgc gcatgccggt tttgtaacct atcccttcga acaaagctgg ccttgtaaat
     6121 ccagtaagaa ggcgtgccat gattcgtcgt atcgagaagg cagtggtgct tggagcagga
     6181 acgatgggtt cgcggatcgc cgcgcatttc gcgaacgccg ggctgccgtg cattttgctg
     6241 gatattgttc cgccgaattt gcccgcggat gcgccggctg gagagcgcaa taaaatcgta
     6301 cgcgctggat tggatgcggc gaagaaatcg aggcccgcag cgttttttac cgccgcgctt
     6361 gcagaaaaaa ttgccattgg aaacttcgac gatgatctgg cgcgttgcgc cgaggccgat
     6421 tggatcatcg aagtcgttgc ggagaatctg gaaatcaaac ggaaattgct ggcgcgcgtg
     6481 gctcagtttc gcaagcccgg ggccattgta accacgaaca cgtccggctt gcccgtgcac
     6541 ttgatcgccg agggcatgag cgaagagttt cagcagcact gggcggggac gcatttcttc
     6601 aatccgccgc gctacctgaa actggtggaa gtgattcccg gaccgaaaac atcaagtgac
     6661 gtggtcgaat cgttgagcgg gttttgcgat cggcggctgg gcaaaggtgt ggtggtggcc
     6721 aaagattcgc cgaacttcat cgccaatcgc atcggaacgt tttcgatgct gaatgcgctg
     6781 cgcctcatgg gcacgctcgg catgaccgtg gaagaagtgg acgcctgcac ggggccggca
     6841 gtgggctggc cgaagtcggc gacgtttcgc acggcggata ttgtcggtct cgatgtgctg
     6901 gtgcatgtcg tgaagaacat ttacgaaacg gcgcccaacg acgagtcgcg ggagagctac
     6961 aaagttccgg cgctcgtgga ggaaatggcc aagcgcggct ggctcggcga caagaccggg
     7021 cagggttttt acaagaaggt gaggggagaa ggggagaaag aaatactgac gctcgatgtg
     7081 aataccatgg aatatcgcgc gcggcaaaag gcgaaattcg cttcgctcga aatgggcaaa
     7141 gcgattgagg acacccggga gcgattgcgc gcgctggtgg ggcccgtgct ggaagggcaa
     7201 aagggtgaca aggcccagca gtttttgtgg ggcgcgcttt ccgagatgtg tttgtatgcg
     7261 gcgcggcgcg tgccggagat ttccgacaac gttgtggacg tagatcgcgc actacgttgg
     7321 ggctttgcct gggaactggg gccgttccag atcatggatg cgatcggcgt caaggcgttt
     7381 gccggacagg tgcagaaaga agggcgcgcc ctgccggcgg tcatcgaaaa gatgctggcg
     7441 agtgggcgga agggatttta cgaatcggaa aaaggtacga cgaccgtttt cgatattggc
     7501 atcggcgcag cgaaaaaagt cgaagagccg aagggtgtca tcattctgaa atcgctgaag
     7561 gatgcgggcc gcgaagtgga gcgcaattcc ggcgcgagcc tgatcgatct cggcgatggc
     7621 gtggtgtgct gcgaatttca cgccaagatg aacgccatcg gagcggacct gatcgccatg
     7681 ctgcacaagg gcttgaagcg cttggaaacg gatttcgacg ccatggtcat cgccaaccag
     7741 gcggtgaatt tttcagttgg tgcaaacttg atgctggttc tggtgggagc ccaggagcag
     7801 gagtgggacg aattgcatat ggcggtaaaa caattccaga acatcaacct ggcgatcaaa
     7861 tacgcgccaa agccggtggt ggcggcgcca cagggtatgg cgctgggcgg cggctgcgaa
     7921 gtgagcctgc acgcggcaaa gattcaggca gcagcggaag catacattgg cctagtggaa
     7981 gcgggtgtgg ggctgattcc aggcggcggc ggcacgaagg aaatgctcat ccgtgcgaac
     8041 gaacacgctg ctggcggaga agatctggat ttgttccacg cgttgaagcc ggtgtttgaa
     8101 gccattgcca tggccaaggt cggcacaagc gcggaggaat gccgcgacct gggatatttg
     8161 cgccgcgaag atggcgtctc catgaaccgt gaccggctgg ttggggatgc gaaagaagcc
     8221 gcgctggcgc tcgtgcgcgg aggcttcaag cctctcgccg caacctggca ggaaggcgcg
     8281 caaaccacgc agatcaaagt gctcggcgag caatttcttg ccggcgcgaa gctcgccatc
     8341 cacatgatgc tccgcggcgg ctatgcctcc gagtatgacg cgcacgttgg ccgcaaactc
     8401 gcgaacatcc tggcgggcgg cgcgctgacg tcaccccaat tggtcagcga gcagtacgtc
     8461 ctcgacctcg aacgcgaagc cttcgtctcc ctctgcggag aaaagaaaac acaggaacgc
     8521 atcgcgcata cgctcaaaac cggcaaaccc ttacggaact aggaagacta aactccacga
     8581 atcgtataaa aacatattgc tatagcgatt aaatatacag tattctgcaa attcgcagga
     8641 ggcggatcgt gcagaaactc ttggcgccgc gggaagcggc gaatattctg ggcatcagct
     8701 atcccacctt gaagcagtgg atttaccacg gaaaaatcaa atcgatgaaa acgcccggcg
     8761 gacaccaccg cgtgccggaa agcgagatcg accgcctgtt tccgaagaag ctggcgcgcg
     8821 gggacttcga gacgcggcgg ggaaatttcc gaaagatcag cgggcgcaat caactgatcg
     8881 ggcgcgtcgt ggcgatcaag ttcagcggat tgcttgcgca ggtcacgctg gccatcggag
     8941 aacaacatat tacctcgatc atcacggcgg atgcggcgaa agagctgcgg ttgaagcccg
     9001 gggagcgggc tgccgcgctg attaaatcga ccgaagtcat gatcctgaga gtgtgaaaag
     9061 agcagtgact cgcggctggt gcctggtgat tggtagaaaa acaaactcgc gcaagtggtt
     9121 ccgtgtcttt caactcattc tttctgtttt ccttgcgcac caagaggtgc gagcccaagg
     9181 aaaggaaatc cgcgtagcgg cggcggcgga tttgaaattt gcgatgggag aattgtcgga
     9241 acagtttgag aagcacacgg gaaccaaagt gaatgtgact tacgggtcca gcggaaactt
     9301 tttcttgcaa atgcagaatg gggcgccttt cgacttgttc ttttccgcgg acattgagta
     9361 tccgagaaag ctggaggccg cgggactcgc ggagccgggg accttctatg aatacgccat
     9421 tgggcgaatc gtgatttgga cgcccgcgga tgcgaaagtg gatgccacca aacagggatg
     9481 gaaactcttg ctggatgcca gcgtcgagaa gattgcaatc gcgaatccgg agcatgctcc
     9541 ctacgggcgc gcagcggtga cggcgctgca aaaggcggga atctacgaat ccatcaaggc
     9601 caagctggtc tacggagaaa atatttcgca agcggcacag tttgtgcagt cgggaaatgc
     9661 gcaagcggga attatggcgc tgtcgctggc ggtttcgccg gcgatgtgcg acgggaaacg
     9721 atgggagatt ccggcggaga tgcatcctgc gcttgagcag ggagcgatta ttttaaagga
     9781 cgctaaaaat aaggatgcgg ctcgtgcgtt tctggaattt gtgaagagtg cggcagggcg
     9841 cgccacgctg gcgaagtatg gttttgaatt tccagagaag atgagcaaag aagaaaagag
     9901 atagcgcaga gacgcagaag gcacagaggc ggagtagaaa aaaggacaac gaatggaagc
     9961 attgtggttg tcgatgaagt tggcgggctg cgtggcggcg atattgcttg tgatcggtat
    10021 gccagtagca tactggctgg cgtattcgag ctggcgcggt aaattcctgc tcgaatcgct
    10081 ggtggcactg ccgttggtgc tgccgccgac ggtgctcggg ttttacgcgc tcgtggcgat
    10141 ggggccgcgc ggaccacttg gaaaactatg gatcgtgctc ttcgggcacg ggctggcttt
    10201 tacgttcggc ggactgattc tggcgtcggt gctgtacagc tttccgtttg cagtgcagcc
    10261 actggtcgct tcctttgagg gagtcgatcg aaaactgctg gacgcgtcgg cggtgctggg
    10321 cgcgggaggc ttgcggacat tttggcgagt aatcctgccg ttgtcgctgc ccggagtcgt
    10381 gacagcggca gtgctgagtt tcgcgcacac cctgggagaa ttcggtgtgg tcttgatggt
    10441 aggagggaat ttggcgggcg tcacgcgaac ggtctcgatc gacatttacg atcgcgtgca
    10501 gtcgctggaa tacggcgtgg ccaaccgcat ggcgctggtg cttctggcga tttccttcgc
    10561 cgtgttgtcc gtagtctatg cggtgaatcg ccgcgtgtgg acgccgtggg cggcgaagta
    10621 aacgccacga tgctcaccgc gcgaatccgc aaagagcggc acaacgcggg cgcctcttcc
    10681 tttgtcct
//
LOCUS       DASYVO010000186        13865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_174324, whole genome shotgun sequence.
ACCESSION   DASYVO010000186 DASYVO010000000
VERSION     DASYVO010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 13865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13865
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174324"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /gene="sdhA"
                     /locus_tag="VGR03_06220"
     CDS             complement(<1..1324)
                     /gene="sdhA"
                     /locus_tag="VGR03_06220"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase (quinone) flavoprotein
                     subunit"
                     /protein_id="HEV1993905.1"
                     /translation="MSTIRAAESGVSVELFSIVPVKRSHSVCAQGGINAAKNLKGEGD
                     STFKHFDDTVFGGDFLANQPPVKGMCEAAPGIVDMLDRMGVMFNRTGEGLLDFRRFGG
                     TLFHRTAFAGATTGQQMLYALDEQVRRYESEGKVKKYEGWTFLSAVLDQGRVCRGICA
                     MDLKTMEVRTFPADAAIFCTGGIGSIFGQSTNSVVCTGSAQSALFQQGVDYANGEFIQ
                     VHPTAIPGEDKCRLMSESARGEGGRVWVPKKQGDTRPPRQIPEAERFYFLEEWYPKYG
                     NLVPRDIATRAIHKAVYEQKMGLGGQPAVYLDVTHIPRETLNRKLEGILEIYEKFLGV
                     DPREEPMKVFPAMHYTMGGIWVDNDHQATNVAGIYAAGECEYQYHGANRLGANSLVSC
                     IYGGMVAGPAAIQYARGLDKGWESVPSNVFETERTRQHYINESLLHQEG"
     gene            complement(1369..2019)
                     /locus_tag="VGR03_06225"
     CDS             complement(1369..2019)
                     /locus_tag="VGR03_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993906.1"
                     /translation="MSAARGDVDPSFFWRKLHSLSGIVPVGAFLAEHFWSNSYVLVSR
                     GKYDQVSQDLQTIPFRIAVEAAVIWIPILYHSAFGFYIWYQGKSNVLQYPWMSNWMYS
                     LQRWTGIVAFFFIGWHLYTARFLTAGRSNFTTVHNDMSNNLYALLYLIGVLAASFHLG
                     NGVWNFLCKWGLAATARAQRAAGFLGAAIALSFSLAGLAIVVGARYDWHPFQSYIQ"
     gene            2284..2820
                     /locus_tag="VGR03_06230"
     CDS             2284..2820
                     /locus_tag="VGR03_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV1993907.1"
                     /translation="MSDTAVAEFTRLVDALVKQGSPIDMATLSKLAVEIGKPFGVKDD
                     EVAILTTTADEKFLQFVLPLALQKIGTIPVTSTSALAARTAREKRPELINNFTIARHS
                     TVFEAVPLEEKHRGDPIQKIMSAPVIQEGKVTGVVQVSRKGKTVTGAGPDFVPRELTE
                     LTGYAAQVGRCIKLLVLE"
     gene            complement(3336..4676)
                     /locus_tag="VGR03_06235"
     CDS             complement(3336..4676)
                     /locus_tag="VGR03_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HEV1993908.1"
                     /translation="MPIRTAIDLVLRDVTYSNAMPDWFVPRQVDHSARKNAIEIRIDQ
                     YLGGTSPGKPFEVLIPRKRSGVKTRWLQPSANDQMILQACVSALAPKIDDSFDRKRVF
                     SYRFEKDPNSLQLTEDQCSAWDDFKTETAAQAGTHIMLMDLEQSFASIDRERFLRFLK
                     QFSTGIEVEILERLLTGFAPSEPGLPLVNDSLFFLGNAYFSVVDKLIAKHTKDFRRFV
                     DDYYVFGASKADLESLYKAINKELQVENFKVNELKLNIISVEDYCDALSKARALVSKD
                     NISLDETDAYGRVLKDVDPNVVLVSLAHTVKQPEKYLNDGIGRFQLASLRKLRDSSPN
                     WSEHKLETSFADRVEVIQPGIQLLKTYTQDAKENWRSIWLLYVMKDLDPKAVKNNQLR
                     RDLQQTLEGIQNSETVPQVVKLWAKVRPVAGPKSKFEELGDADYEEAGRRCCGG"
     gene            complement(4706..5758)
                     /locus_tag="VGR03_06240"
     CDS             complement(4706..5758)
                     /locus_tag="VGR03_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993909.1"
                     /translation="MIAAPILFLVVDFCRIPVLLSGCQGGKEEITLRTLVEKQTALLG
                     SYLGHRFYVSLDAIQDSQYLHFLKTAPTALVIGIDHDKPSEPSWGGLTRAVLRKFDEI
                     SLEDAAMLRSVVLGSDPVSGHLLAKPLGIPSARFGGFPVNFLYIVGIKSRGDGSGPAK
                     KQQADTLKSGLQDVMAQAKRDKIENLILPCVSVDPRDPATLQYHEYFPLVFIVSEPAW
                     RPNSIYLSLYQDWDDTSRSAALRALQTSWTETCANAGKQSLFVREVLRLMLLTLVVCL
                     VVSSLYVAISVKNFLIISCSFVALGSGALALLSPFVENWNSSARFGAHVTLLVALAVL
                     FPLLPRFNPKEIFGKR"
     gene            6949..7506
                     /locus_tag="VGR03_06245"
     CDS             6949..7506
                     /locus_tag="VGR03_06245"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HEV1993910.1"
                     /translation="MAFVAKRIFLTKGVGKHRERLSSFELALRNAGIAACNIVRVSSI
                     FPPQCKLISRAEGLKHVKPGQVAFTVISENSTREPHRLIAAGIGVALPADKSMYGYLS
                     EHHSFGETEENAGEYCEELAAEMLATTLNVEFDPDLSWDEKKEVYRISNKIVRTLNIC
                     QSAVGDKRGLWTTVLSAAFLLGEDD"
     gene            complement(7647..7994)
                     /locus_tag="VGR03_06250"
     CDS             complement(7647..7994)
                     /locus_tag="VGR03_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993911.1"
                     /translation="MDASVVMPNHMHGIVIIFARARSRPCGMPLREEKKQTEFGKPLA
                     GSLATIVRSFKAAVTKFARERRLIHAHPVWQRGYYERVIRTGEEYSDTSRYILVNPLR
                     WGTDAENPDLKAK"
     gene            complement(8282..9370)
                     /locus_tag="VGR03_06255"
     CDS             complement(8282..9370)
                     /locus_tag="VGR03_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEV1993912.1"
                     /translation="MKQKKPGKKKATQKKTVTGRILHDPIRDHLQPIYPLDLSKCRTV
                     DALVRAMGQTAFTGRQIGDAADILEAMARDEDCFVVLTLSGAMTVAKMGLIFCDLMES
                     GIVKAIVSTGALMAHGLVEATGRSHFRYDPKMDDRELFQAGYNRVYDSLEPEVNLDHV
                     EQVVDHVLDGWDASETVCSWKLHRRIGEYLLHESSGRGILKTARECNVPVFVPAFTDS
                     ELGIDFALHKRSRIAKKLPLLRFDPFEDFEYFAKTMLASKKMGIFTVGGGVPRNWSQQ
                     FGVYAELLARRGYEKIPLKRYHYGLRICPEPVHWGGLSGSTYREAVSWGKFVPEEEGG
                     KFAEVFDDATVALPLVVGAVLERLRYFK"
     gene            9514..9792
                     /locus_tag="VGR03_06260"
     CDS             9514..9792
                     /locus_tag="VGR03_06260"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1993913.1"
                     /translation="MTHIGMWGVYVDVGVVGWLIIGLLAGWLSGEITRGKGFGCLGNT
                     LLGLIGAVLGGWLFSLLHISAYGFVGGLAAATVGAVVLVSIARAIAGS"
     gene            9890..12643
                     /locus_tag="VGR03_06265"
     CDS             9890..12643
                     /locus_tag="VGR03_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV1993914.1"
                     /translation="MPDPSSILGRTISHYRILEMLGGGGMGVVYKAQDMRLDRAVALK
                     FLPEDLARDHQALERFQREAKAASALNHPNICTIHDIGEEDGRAFIVMEFLEGSTLKH
                     CIGGRPVGIETLLDLASEISDALDAAHAHGIVHRDIKPANIFVTQRGHAKVLDFGLAK
                     QLRLENFEGGGETASLAAKAKSEIRAEALTSPGVAVGTVAYMSPEQVRGKDLDARTDL
                     FSFGVVLYEMATGVLPFRGETSGVITEAILNRAPVAPVRLNPDLPAKLEELINKALEK
                     DRELRYQSAAEMRADLKRLRRDTDSGRHSSSASAAAQVIVPAPARDASATVAEQYSTG
                     RASTGPASGGLALGGLARRKSILIGAGVLLLAGALATYRFWARPGAPRGAAKITQISH
                     WNKPMVGAKLSPDGHTVTFISQVGTIDQVFVMLSSGGDPLQLTNDEGNKLVDSFSPDG
                     THIYYARSLGRYEVWAVPTLGGTPSRVASGTLLVPSPDGGSFFYIKPDGLAIVRAGKS
                     GLGEELVYSLKKDSGLTPRLLLTYPGGNDLLLIATIGSVAGSNCRIFKVNVASHNADA
                     LGEISGDPQGFVWGEPGKTLLFSRSVNDLTNLWEYNLVDRSFTQITSGPGPDLSPMPD
                     PAGKGIYYVNGKTSGLLTTYHVPSKDSADILPEEATQPAISRDGKRVMYLRVPQINRT
                     EVWVSDIDGKNKVKLFSSGILNIGDWAHDGSQISFEDDTGGEAKAYAIGADGSGLRQF
                     PRMGNFVNYSTWGADGKALYINGFDKDGVHPTTWKANADGSSVEAFVEDCGYVADASS
                     DGEYLLSTQPAGEKTGIYVISLVDKKCSLVLPGVAVDFPHFALDGKSFQYAMALHGEM
                     IVYRQPLRDGKAAGPVQIALKLPFVLREDYGGNAYDISRDLSTIVYAHPSGQADLYLL
                     SRK"
     gene            12755..>13865
                     /locus_tag="VGR03_06270"
     CDS             12755..>13865
                     /locus_tag="VGR03_06270"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1993915.1"
                     /translation="MSQKSNSSSASSGARAAVLAVPIGGLGEFGMNMMAYRSGDEILV
                     VDAGLMFPEAELLGVDVVLPDITYLKQNIAAVRAIVLTHAHEDHIGALPYLLPDLNVP
                     VYGTRFTLAMLRKKLGESGHLEDATLREMVPGDKISIGAFEVEPIHVTHSTIDCVALA
                     IRTPAGVIIHTGDFKMDPTPTDGRPFDLHTLARYGQEGVLALFSDSTNAERPGYTPSE
                     RAVRPQLEALVRAAPRRVIVSCFSSSIHRIQQVVDISHQLGRKVAFVGRSMVDNVEIA
                     HSLGQITIPDGMVVRQQDIKGFDPRKIVVLASGSQAEAMSALSRLAVDNHRMLSLEEN
                     DSVILSARIIPGNEKAISRMIDHLFRRRALVYYEQG"
BASE COUNT         2939 a         3923 c         4196 g         2807 t
ORIGIN      
        1 tgccctcctg gtggagcagg ctctcgttga tgtaatgctg gcgggtgcgc tcggtttcaa
       61 atacgttgga aggcacgctc tcccagcctt tgtcgaggcc gcgggcatac tggatggcgg
      121 cggggccggc caccatgccg ccatagatgc aggagaccag cgaattcgcg cccaggcggt
      181 tggcgccgtg atactggtat tcgcattcgc cggcggcgta gattccggcg acgttggtgg
      241 cctggtgatc gttgtccacc cagatgccgc ccatggtgta gtgcatggcg ggaaaaactt
      301 tcatgggctc ttcgcgcggg tcgacgccga gaaacttttc gtagatttca agaatgcctt
      361 cgagcttgcg gttgagcgtt tcgcgcggga tgtgggtgac gtcgagatag acggcgggct
      421 ggccgccgag tcccatcttt tgctcgtaga cggccttgtg aatggcgcgg gtggcgatgt
      481 cgcgcggcac caggttgccg tacttgggat accactcctc gaggaaatag aagcgctcgg
      541 cttcgggaat ctggcgcggg gggcgagtgt cgccctgctt tttggggacc cagacgcggc
      601 cgccttcgcc gcgcgccgat tcgctcatca ggcggcactt gtcctcgccg ggaatggcgg
      661 tgggatgcac ctggatgaat tcgccgttgg cgtaatcgac gccctgctgg aacagggcgg
      721 actgcgcgga gccggtgcaa accacggagt tggtggactg gccgaagatg gagccgattc
      781 cgccggtgca aaagatggcg gcgtcggcgg ggaacgtgcg gacctccatg gttttcaaat
      841 ccatggcgca gatgccgcgg cagacgcggc cctggtcgag gacggcggag aggaaggtcc
      901 agccctcgta ctttttcact ttgccttcgg attcgtagcg gcgcacctgc tcgtcgaggg
      961 cgtagagcat ctgctggcca gtggtggcgc cggcaaaggc ggtgcgatga aagagcgtgc
     1021 cgccgaaacg gcggaaatcg agcagacctt cgccggtgcg attgaacatg acgcccatgc
     1081 ggtcgagcat gtcgacgatg cccggcgccg cttcgcacat gcctttcacg ggaggctgat
     1141 tggcgaggaa gtcgccgccg aagacggtgt cgtcaaaatg cttgaaggtg gagtcgccct
     1201 cgcctttgag attcttggcg gcgttgatgc cgccctgcgc gcagacggag tgggagcgct
     1261 tgaccggaac gatggagaac agctcgacgg aaacgccgga ttcggccgcc cggatggtgg
     1321 acatcaggcc ggcgagtcct ccgcccacga caacaatccc gggttgtctc attggatgta
     1381 agactggaag ggatgccagt cgtaacgcgc gcccacgacg atggccagcc cggccagcga
     1441 aaacgacagg gcgatggcgg cgccgaggaa tccggcggcg cgttgcgcgc gcgccgtggc
     1501 ggccagcccc catttgcaca gaaaattcca gacgccgttg cccaggtgaa aggaggcagc
     1561 aagaacgccg ataagataca ggagcgcata gaggttgttg gacatgtcgt tgtgcacggt
     1621 ggtgaaattc gagcggccgg cggtgagaaa gcgcgcggta tagagatgcc agccgatgaa
     1681 gaagaaggcc acgatgcccg tccagcgctg gagcgaatac atccagttgc tcatccacgg
     1741 gtactggagg acgttggatt ttccctgata ccaaatgtaa aagccgaagg cgctgtgata
     1801 gaggatggga atccagatga ccgcggcttc gacggcgatg cgaaatggaa tggtttgcag
     1861 gtcctggctg acctggtcat attttccgcg gctgaccagg acgtagctgt tggaccagaa
     1921 atgctcggcc aggaaggctc ccacgggaac gattccggag agagagtgca gcttgcgcca
     1981 gaaaaagctg gggtcaacgt caccccgtgc ggcagacata atctctatta cgctcccggt
     2041 cgcggattca gacgagcctg cccgcgggcg gatcgtctcc ctgcgggtct cctgcaggac
     2101 tccatccagg cgaaagcacc tttttgaagg gttcgcacgg agttcacgca ggaattgcag
     2161 tatatcgccg cccccggcgg ttcgcaagcc gcgggcggac ggaagaagaa agaatttcac
     2221 tgaaacgcgc gggccggttt cgttgtaaga tttgccctgt tgatcaccgg agaggaggca
     2281 ggaatgagtg ataccgcggt ggccgagttc acccggctgg tagatgccct ggtcaagcag
     2341 ggatcgccga tcgacatggc cacgctgtcg aagctggccg tggagatcgg caaaccattc
     2401 ggcgtgaaag acgatgaagt ggccatcctc accacgacgg cggatgagaa gtttttgcaa
     2461 ttcgtgctgc ccttggcgct gcagaaaatc ggcaccatcc cggtgaccag cacgtcggcg
     2521 ctggccgcgc ggacggcgcg ggaaaaacgg cccgagctga tcaacaactt caccattgcc
     2581 cggcattcca cggtttttga agcggtaccg ctggaggaga aacatcgcgg cgacccgatt
     2641 caaaagatca tgagcgcacc ggtgattcag gaaggcaaag tgaccggggt ggtgcaggtt
     2701 tcgcgcaaag gcaagacggt taccggcgcc ggaccggact tcgtccccag ggaactgacc
     2761 gaactgaccg gctatgccgc acaggtgggc cgctgcatta agcttctcgt cctggaatag
     2821 cgatgtttcc cgcccgagac ccgacagcgc ggcgggtccc tctctacaat gttctcagcc
     2881 tcgccgcggc gctgggatgc gcggcgattg gcgccggcag cattcccccg cagaatccgc
     2941 gccacgtttc gtttattagt ctctttgtgc ggaaactaac agagtgactt cgtatcgttt
     3001 ccagggaacg gcgcaagggt caaccagtga gaggtaagaa cgacagatct tggagcgacc
     3061 gacaatcgga acgaattgct tgggtacaaa ccctagctga cgaccatcca cgtcgtaaac
     3121 gggatgacat cttttcccgt cgaaactact actgccgata atgaccgggt ttccgtcttg
     3181 tagtcccgtc tggacccggt aaaagcgaac acccgccagg taaaaggaga tgctttcgga
     3241 ggcgccggcc gcctcctcgg gccacgcgaa ttgcggtacg gcaagcagac cacagcaagt
     3301 ggtcgcgcat ttaagaaatt ctctcctatc catcctcatc ctccacaaca gcgtctgcct
     3361 gcctcctcat aatcagcgtc accgagctct tcaaacttgg atttcggccc tgccaccgga
     3421 cgcaccttgg cccagagctt tacaacttgt ggaactgttt cagaattctg aatcccttcc
     3481 agggtttgtt gcaggtccct ccggagctga ttattcttca cggcttttgg atcgaggtct
     3541 ttcatcacgt aaagcaacca gatgctgcgc cagttttcct tggcgtcttg ggtatacgtc
     3601 ttcaagagtt gaataccagg ctgaattact tccacacgat cggcaaagct agtctccagt
     3661 ttatgttcgg accaattggg ggaagagtcc cgcagtttgc ggagcgaggc aagctgaaat
     3721 cggccgatgc catcgttcag atacttttca ggctgcttga cggtgtgggc cagcgaaacc
     3781 agcacgacat tcggatcgac atccttcagc acgcggccat aggcatctgt ttcatccaac
     3841 gagatattat ccttgcttac caacgccctg gccttggaaa gcgcatcgca atagtcctcc
     3901 acgctgatga tgttcaactt caactcgtta accttaaaat tttcaacctg taattccttg
     3961 ttaatggcct tgtacaagga ctccagatca gctttggacg ctccaaacac atagtagtcg
     4021 tctacaaacc gacggaagtc cttggtatgc ttggcgatca gtttgtcaac cacactgaag
     4081 tatgcgttcc ccaagaagaa gagagaatcg ttgaccaacg gcagccccgg ctccgaaggc
     4141 gcaaagccgg taagcaaccg ctccaggatt tcgacctcga tcccagtgga aaactgctta
     4201 agaaaacgca aaaaccgctc gcggtcaata ctggcaaacg actgctccag gtccatcagc
     4261 atgatgtgcg ttccagcctg ggccgcagtc tccgttttga agtcatccca tgcggaacac
     4321 tgatcttccg tgagctgcag ggagttcggg tctttctcaa aacgatagct gaacacgcgt
     4381 ttccggtcga aactgtcgtc aatctttgga gccagtgcag aaacacatgc ttgcaggatc
     4441 atctgatcat ttgccgaagg ctgcagccat ctcgtcttga cgcccgatct ctttcgtggg
     4501 atcaagacct cgaacggctt ccctggtgaa gtgccgccga gatactgatc aatcctaatc
     4561 tcgatggcgt tcttgcgtgc cgagtgatcg acctgcctgg gcacaaacca atcgggcatg
     4621 gcgttgctat aagtgacgtc gcgcagcaca agatcgatcg ctgttcgaat cggcaagtcc
     4681 gcaatagcgc tgttcatgga agatcctacc ttttgccgaa tatctctttt gggttaaacc
     4741 tgggcagaag cggaaacaat accgcaaggg cgacaagcag agtcacatgt gcgccaaagc
     4801 gagcgcttga attccagttt tcgacaaaag gggaaagtaa cgcaagggcc ccggaaccca
     4861 gggccacaaa tgaacagctg atgataagaa agtttttcac gctaatcgcc acgtacaagg
     4921 aactcaccac caggcaaacc accagcgtca acagcatgag cctgagtacc tctcggacaa
     4981 acagcgattg cttcccggcg ttggcacagg tttccgtcca ggaggtttga agcgctctta
     5041 gagcagcgga tcgagaagta tcgtcccaat cttggtagag ggacagataa atgctattcg
     5101 gtctccaggc tggttccgac acaataaaca ccaaagggaa gtactcatgg tattgaagag
     5161 ttgccggatc acgagggtct acgctcacac acggaagaat cagattttcg atcttgtctc
     5221 gtttagcttg cgccatcacg tcctgtagtc cacttttgag cgtatcggct tgttgctttt
     5281 tcgccggacc gcttccatcg ccccgggatt tgatccccac gatgtacagg aaattgacag
     5341 gaaagccccc aaagcgcgca gaggggatac ccaaaggttt ggccaggaga tgacctgaaa
     5401 cgggatcgct acccagtacc acacttcgca acatagcagc atcttctaaa gagatctcat
     5461 cgaatttccg caggactgcc cgggtgagac ctccccaaga aggttctgag ggcttgtcat
     5521 ggtcgattcc aataaccaag gccgtgggtg ctgtcttaag gaaatgcaaa tactgcgaat
     5581 cttgaatcgc atcgagtgac acgtagaatc ggtgtcccag gtaggaacct agaagagctg
     5641 tttgcttttc caccagcgtt ctgagcgtaa tctcctcttt acctccttgg cacccgctga
     5701 ggaggaccgg aatacgacag aagtccacca ctaagaatag gatcggtgct gcaatcaaga
     5761 agacttgcgc cgagcgcctg gtccatgact tggaaggcga ggcaagcgtg ggtggacgca
     5821 ttctggatgc ctatggaact acccgcttgg gtatcacaat tataagacat gtcaggtaca
     5881 aagtgggggt gtgttggttc agcctgaaat ggagagaggg cgctctccgt tagtgagtat
     5941 tccgccctgc ggggcgggga ttttcaaggc atcgatacga ccaccaggct atccctcagc
     6001 atggttgcgt ccccgcagag cccgcttccg tttcgcccgg caagatcatt gtagctccac
     6061 cacgtccatc cgtctcaact tccggttagc ccgtgactcg cccagaatcg gcggcgagca
     6121 ccaagtgagc ttttgcatct cacgacagcg tttcgcacag caattcaaat tgccaaactg
     6181 tgttgcgtct caaatgggga cagacgctga gctggttgac cgcagcctct ggagagatta
     6241 tacctttcac tgctcatggc ctctcgccgc attgaagtct tcttctacgg gttattcatg
     6301 gacgccgacc tcctgaggac gaagggggtc aatcctacca acattcgccc agcttccgtt
     6361 cccggcttcg cgctgcggat tggccaacgg gcaactctgc tgcggaatcc caatcgcctc
     6421 gcgcacggca tcctgatgga actcactcac acagaggtcg agcaactgta ttcagaactc
     6481 agcgttcgcg cttatcgtcc agaggcggtg ctatgtgagc ttaacgacgg ttcacgtatt
     6541 cccgctttgt gcttcaatct ccttgagccg ccacgcgctg acgaggccaa ctcagaatac
     6601 gcatcgaagt tgcgtgacct cgcactccgg ctagggcttc cctctgctta tgtcgatact
     6661 attcaatgaa acttcgctgt gaacggtagc gtttgatttc gattgctcga cagcgccgcc
     6721 tgccgcaacc catcccgatt tgcgtcctcc agggattgca gcatcactaa gactcaaacc
     6781 aaaaaccagg catatacccc cccacccccg gaggagggca tttctacttt gccttgacac
     6841 gccctacaaa tggattgaca ctggtgcagt ctcttgttac ttttcgcgcg ggatttttct
     6901 cacaaggagg ccgcgcaagg ttcccgcaag aagcgcaacc gacacacgat ggcgttcgtc
     6961 gcaaaaagaa tatttctaac caagggcgtc ggaaagcacc gcgaaaggct ttcgagtttc
     7021 gagctggcgc tgcgcaacgc cggcatcgcg gcgtgcaaca tcgtgcgcgt gtcgtccatt
     7081 ttcccaccgc agtgcaaact gatttcgcgt gccgaagggc tgaagcacgt gaaacccggg
     7141 caggtggcgt tcaccgtgat cagcgagaat tcgacgcgcg agccgcaccg gctgatcgcc
     7201 gcagggatcg gcgtggcgct gccggccgac aaatccatgt acggctacct ctccgagcat
     7261 cactcgttcg gggagaccga agaaaatgcc ggcgaatact gcgaagagct ggcggcggaa
     7321 atgctggcga ccacgctcaa cgtggagttc gatccggatc tttcgtggga cgagaaaaaa
     7381 gaggtttatc gcatctcgaa taagattgtc cgtaccctga acatctgcca atcggcggtg
     7441 ggcgacaagc gcggcctgtg gaccacggtt ctctccgcag cgtttcttct cggcgaagac
     7501 gattaaacgt ttttcgtagg gcacggcatg ccgttaccgt acgccccgcg cccgaatgca
     7561 atcccagacg ggcacggcat gccgtgcccc tacgacccta cccgtgtccg gaattccttt
     7621 cgaaaaacga tttgctcgcc ggtagttcat ttcgccttca aatccggatt ctccgcatcc
     7681 gtgccccagc gaagcgggtt caccaagata tatcgagagg tatccgagta ctcttcgccg
     7741 gtgcgtatca cccgttcgta gtatcctcgc tgccaaacag gatgcgcatg aatcagacgc
     7801 cgctctcgcg cgaatttcgt cacggctgcc ttgaaggaac gtacgatggt tgccagcgaa
     7861 cccgcgagcg gcttcccgaa ttcggtttgc ttcttttctt cccgtagggg catcccgcat
     7921 gggcgggacc gtgcccgggc aaagatgatc acgattccgt gcatgtgatt cggcatcacg
     7981 acactcgcgt ccaattcgac tcccgcgaaa tgacggggaa tctctgttca acattcgcgg
     8041 accatcctgc cgatggaatt caaagacatc ttgccatcga cgatcgagcc gaagagcagc
     8101 ctgcgttggt acgagcaaat ggcaacgcaa taaagacctg cctcgaaata gtcgatcccc
     8161 ttcagtcgaa tggaacgcct ctgcgggatg agttcgtgcg acatcgggcg atcgaggatg
     8221 caacgattcc gatcttacga gattgctatc ggggcccagt cgggcacggc atgccgtgcc
     8281 cctacttgaa atagcggagg cgttcgagga cggcgccgac gacgagggga agcgcgacgg
     8341 tggcgtcgtc gaagacttcg gcgaatttac cgccttcctc ttcagggaca aatttgcccc
     8401 aggagaccgc ttcgcggtag gtgctgccgg acaggccgcc ccagtgcacc ggctcggggc
     8461 agatgcgcag gccatagtgg tagcgcttca ggggaatctt ttcgtagccg cggcgcgcca
     8521 gcagctcggc gtaaacaccg aattgctgcg accagttgcg cggcacgccg ccgccaacgg
     8581 tgaagatgcc catctttttc gaggccagca tggtttttgc gaaatattcg aagtcctcga
     8641 aagggtcgaa gcgcaacagc ggaagcttct tggcgatgcg gctgcgcttg tggagagcga
     8701 agtcgatgcc cagctcggaa tcggtgaacg cggggacgaa gacggggacg ttgcactcgc
     8761 gcgcggtctt caggatgccg cggccggatg attcgtgcag cagatattcg ccgatgcggc
     8821 gatggagctt ccaactgcag acggtttcag aggcgtccca gccatcgagg acatgatcga
     8881 cgacctgttc cacgtggtcg agattgactt cgggctcgag cgagtcgtag acgcggttgt
     8941 agccggcttg aaaaagctcg cggtcgtcca ttttggggtc gtagcggaag tgcgagcggc
     9001 cggtggcctc gaccaggcca tgggccatca gcgcgccggt ggagacgatg gccttgacga
     9061 tgccggactc catcaggtcg cagaagatca atcccatctt ggccacggtc atggcgccgg
     9121 agagcgttag gaccacgaaa caatcttcat cgcgggccat ggcctcgagg atgtcggcgg
     9181 cgtcgccgat ctgccggccg gtgaaggcgg tctgtcccat ggcgcgcacc agggcgtcga
     9241 cggtgcggca tttcgagagg tcgaggggat agatgggctg caagtgatca cggatggggt
     9301 cgtgcaaaat ccggccggtg acggttttct tctgcgtcgc ttttttcttg ccgggttttt
     9361 tctgcttcat ccagcggcct cgcgcatatt tttcaacttg cgttcccgtt tgcgagtcct
     9421 gatagtacaa gacttcgcgg cgaagacaag agagagtgcg cgcgtggtag aggacattcc
     9481 ggtcgcgcgc gtcgcaaaaa acaaaggtag ggcatgacac acataggaat gtggggagtt
     9541 tacgttgacg ttggagtggt gggctggctg atcatcggat tactggccgg atggctgagc
     9601 ggcgaaatca cacgcggaaa aggattcggc tgcctgggca atacgctgct gggattgatc
     9661 ggagcggtgc tgggcggctg gctgttttcg ctgctgcaca tcagcgcgta cggattcgtc
     9721 ggcgggctgg ccgcggcgac cgtgggcgcg gtggtgctgg tgtctatcgc gcgggcgatc
     9781 gccggcagct aggaacattg aaaagccctg ccggacacgt tcgctaaatg gctccatgga
     9841 ttgcggcaac gttgcctgta cgcgcactcc gtcgtaagat gcatgaccaa tgccggatcc
     9901 ctcctccata ctcggccgaa ccatctccca ctaccgcatc ctggaaatgc tgggcggcgg
     9961 cggtatgggc gtggtgtaca aggcgcaaga catgcggctg gaccgtgccg tcgcgctaaa
    10021 attccttccg gaagatttgg cgcgcgacca tcaggcgctg gaacgattcc agcgcgaggc
    10081 gaaggctgct tcggccttaa accacccgaa catctgcacc attcacgaca tcggcgaaga
    10141 agacggccgc gcttttatcg tcatggagtt cctggaaggc agcacgctga agcactgcat
    10201 cggcggccgt ccggtgggaa tagaaactct gctcgatctg gccagcgaga tttccgacgc
    10261 gctggacgcg gcgcatgcgc acggcatcgt tcatcgcgac atcaagcccg ccaatatttt
    10321 cgtcacccag cggggacacg ccaaggtcct ggattttggc ctggccaaac agcttcgcct
    10381 ggaaaacttc gaagggggcg gagagacggc ctcgctggct gcgaaagcga agtcggagat
    10441 aagggcggaa gccctcacca gcccaggggt ggccgtgggc acggtcgctt acatgtcacc
    10501 ggaacaggtt cgcgggaaag atctggacgc acgcaccgat ctcttttcgt tcggcgtggt
    10561 gctgtacgag atggccaccg gcgttctgcc ctttcgcggg gaaacgtcgg gagtaatcac
    10621 cgaggccatt ctcaatcgcg cgccggtggc tccggtgcgg ctgaatccgg atcttccggc
    10681 gaagctcgag gaactgatca acaaggcgct ggagaaggat cgcgaactcc gctaccagtc
    10741 ggcagcggag atgcgcgcag acctgaagcg cctgcgccgg gatacggact cggggcgaca
    10801 ttcctcgtct gccagcgcgg cggcgcaggt tatcgttccg gctcccgcaa gggacgcgag
    10861 cgcgacggtg gcggagcaat attccaccgg acgggcctcc accggaccgg cttccggagg
    10921 gctggccttg ggcgggctgg cacggaggaa gtccatcctg attggcgctg gcgtcctact
    10981 cctggcaggc gcacttgcga catatcgctt ctgggcccgc cccggcgccc cgaggggcgc
    11041 cgcgaaaatc acgcagatca gccactggaa caagccgatg gttggggcga agctttctcc
    11101 cgacgggcac acggtgacgt tcatttcgca ggtggggaca atcgaccagg tgttcgtgat
    11161 gctcagttcg ggcggcgacc ctcttcagct caccaacgac gagggcaaca agctggtgga
    11221 cagtttttcg ccggatggga cgcacatcta ctacgcgcgt tccctgggcc gctatgaggt
    11281 ctgggcggtg ccgactctcg gggggactcc gagccgagtg gcctcgggta ctcttctggt
    11341 tccatctcct gacggcggct ccttttttta tataaagccg gatggcttgg cgatcgttcg
    11401 cgcgggtaaa tcagggctgg gcgaagaact ggtttacagc cttaagaagg atagcggact
    11461 cacacctcgc ctgcttctga catatcccgg tggaaatgac ttgctcttga tcgcgacaat
    11521 aggatcggta gccggcagca actgcagaat cttcaaagtg aatgtcgcca gccacaatgc
    11581 agatgccttg ggtgaaatat ccggggaccc gcagggtttc gtgtgggggg aacccggaaa
    11641 gaccctgctg ttcagccgca gcgtgaacga tctcaccaac ctttgggaat acaacctggt
    11701 ggaccggagt ttcacccaga tcacttcggg gccagggccg gatctctccc ccatgccgga
    11761 cccggctgga aaaggaattt actacgtaaa cggcaagacc tccgggttgc tcaccaccta
    11821 ccacgtacct tccaaagatt ccgcagacat tcttccggaa gaggcgaccc agccggccat
    11881 ctcacgggat gggaagcggg taatgtattt gagagtcccg caaatcaatc gaactgaagt
    11941 ctgggtgtcg gacatcgatg gaaaaaacaa agtcaaactt ttttcgtccg ggattctgaa
    12001 tattggagat tgggcgcacg atggtagtca gatcagcttt gaagacgaca cgggcgggga
    12061 ggcaaaagcc tacgctatcg gggcggacgg cagcggtctt cgccaattcc cgcggatggg
    12121 aaattttgtc aactacagta cgtggggagc cgatggaaaa gctctttaca tcaacggttt
    12181 cgataaggat ggcgtacatc caaccacctg gaaagcaaac gcagacggct cgagcgttga
    12241 agcatttgtg gaagattgcg gctatgtagc cgacgcttcc tccgacggag agtatctgct
    12301 ttccacgcaa cctgctgggg aaaaaaccgg catctacgta atatcccttg tcgacaagaa
    12361 gtgcagcctg gtgttgccag gggtcgctgt cgacttcccg cattttgctc tcgatggcaa
    12421 gtctttccag tacgcgatgg ccttgcacgg ggagatgatc gtttatcgtc aacccttgcg
    12481 cgacgggaag gccgccggcc ctgtgcagat cgcgctcaaa cttccttttg tcttgcggga
    12541 ggactacggc ggcaacgcct acgatatttc ccgcgatctt tccaccatcg tttacgcgca
    12601 tcccagtggc caggcggatc tctaccttct gagccggaaa taggtttggc cgcgcgaggg
    12661 caatatgggc acccaaatcc ttccagcgtg acaaccttaa aagccgccgc acctgtgata
    12721 cgatggctga ttgagtgaga cctcggaacc cgcattgagt caaaaatcca attcatcttc
    12781 ggcttcatcg ggggcgcgcg ctgcggtcct ggccgttccc atcggcggac tgggcgaatt
    12841 tgggatgaac atgatggcct accgctcggg agacgagatc ctggtggtgg acgccggact
    12901 gatgtttccc gaagcggagc tgctgggtgt cgacgtcgtt ctcccggaca tcacctacct
    12961 gaaacagaac atcgctgcgg tccgcgccat cgtgctgacg cacgcccatg aagaccacat
    13021 tggcgcgttg ccgtacctgc tccccgacct gaatgttccg gtgtatggca cgcgcttcac
    13081 gctggcgatg ctgcggaaaa agctcggcga gagcgggcac ctggaagacg ccacgctgcg
    13141 ggagatggtg ccgggggaca aaatcagcat cggcgcgttc gaggtggagc cgatccacgt
    13201 gacgcacagc accattgact gcgtggcgct ggcgattcgc acgccagcgg gcgtgatcat
    13261 tcacaccggc gacttcaaga tggaccccac acccaccgat gggcggcctt ttgacctgca
    13321 cacgcttgcg aggtatgggc aggagggagt gctggcgctg ttcagcgaca gcaccaacgc
    13381 ggagcggccg gggtacacgc cgtcggagcg tgcggtgcgg ccgcagctcg aggcgctggt
    13441 gcgcgcggcg ccgcggcgcg tgatcgtttc gtgcttctcg agctcgattc accgcatcca
    13501 gcaggtggtg gacatcagcc accagcttgg gcgcaaggtg gcgtttgtcg gacgcagcat
    13561 ggtggacaac gtcgaaatcg cgcacagtct ggggcagatc acgattccgg acggcatggt
    13621 ggtgcgccag caggacatca aaggcttcga cccgcgcaag atcgtggtgc tggcgagcgg
    13681 cagccaggcc gaggcgatgt ccgcgctgtc gcggctggcg gtggacaatc accgcatgtt
    13741 gagcctggag gaaaacgatt cggtgattct ttccgcgcga atcataccgg gaaacgagaa
    13801 agcgatttcg cggatgatcg atcatctatt ccggcggcgc gcgctggtct attacgagca
    13861 ggggg
//
LOCUS       DASYVO010000187        12753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_105486, whole genome shotgun sequence.
ACCESSION   DASYVO010000187 DASYVO010000000
VERSION     DASYVO010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 12753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12753
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105486"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /gene="pyrR"
                     /locus_tag="VGR03_06275"
     CDS             complement(<1..426)
                     /gene="pyrR"
                     /locus_tag="VGR03_06275"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV1993916.1"
                     /translation="MRIVEKGQLMSAPEIDRTIQRLAHEIVERTGGTKHLALIGIVRR
                     GVPLAQRIAQAMRGIDGLEVPVGVLDITLYRDDLSKVAPQAVLRSSDISFGVDNMDLV
                     LIDDVLYTGRTIRAAMNGLFDLGRPKRVSLCVLIDRGHRE"
     gene            540..2327
                     /locus_tag="VGR03_06280"
     CDS             540..2327
                     /locus_tag="VGR03_06280"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV1993917.1"
                     /translation="MARRTNPIGHRTISALVLLALIFAFPPLTGAQTQPQAATAPKAP
                     AKPKLVVLLVVDQMRGDYVDKFLGQWRGGLKRLVEEGAWFRDAAYPYAATETCVGHAT
                     ISTGALPATHGMVANAWWERKEQKMVTCTSDPDPSVKNIGYAGATPKGADTAWRMAVP
                     SFAEELKFQTSGATRIVTFSLKARAAITMAGHKADAATWFDSGAWVTSTAYGTLPFME
                     EQAKSHQAKADFGKTWSLSLPESAYWYGEKALGAVPPDGWDLTFPHPLRGKSSVGEPD
                     SAFYEQWASSPYADTALTELAEKAVDTLNLGKAAGTDFLAVSYSSVDYVGHEFGPRSR
                     EIQDILVRLDKDLGNLFAHLDQKVGRGNYVVALSADHGVVPIPEDMQKTGADAGVLHL
                     LELQERIEKALEPFKYTKPAIARITGSDIYFAPGVYDKLQQDRAGLQAVLDVALAQPG
                     VAAVYRAEELRDRPATQSPSHRAMANGYFPGRSGDLFILPKPYWLMDGTPLGKPRSYG
                     TGHGTPYNYDQHVPVLLMGFGIQPGQYFQPITPADIAPTFAALCGITLSSRDGHVLAE
                     ALKKPAEHRAPAQPAHPKSAPASPPSTNP"
     gene            2789..3727
                     /locus_tag="VGR03_06285"
     CDS             2789..3727
                     /locus_tag="VGR03_06285"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV1993918.1"
                     /translation="MSSAGAVDLSVKIGALRLRNPILAASGTFGYGLGFAHLVDLNRL
                     GGFVTKGLSREPIEGAPAPRLYPTPSGMLNAVGLQNVGVRAFVAEKLPVLRKFDTAVI
                     ANVFGCTLDDYIQVIRVLEDAEGLAGYELNISCPNVKKGGMQFGSDPIQVAEVVAAAR
                     KAATKRPLWVKLSPLVTDIGLIARAAEEAGADALTVANTYPAMAIDFRSGKSRLGNPT
                     GGLSGPAIKPITLRLVWETKKAVKTPIIGLGGIESVEDVLDYLAVGASAVQVGTASFT
                     DPRASQRLAGTLTKVLLRAKAFTLSEIRDKFLAENG"
     gene            3803..4210
                     /locus_tag="VGR03_06290"
     CDS             3803..4210
                     /locus_tag="VGR03_06290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEV1993919.1"
                     /translation="MSWAALRWVLLMFLIAPGMEAPLVAHLNQPVKPIKTWVGNSSWY
                     GPGFNGRKTANGERFDSEALTAAHPNLPFGSLVRVVNPRNGKFELVRINDRGPYQEGR
                     EIDVSYRVARKIGLIHSGVSQVRLELMELPGKH"
     gene            4234..4983
                     /gene="pyrF"
                     /locus_tag="VGR03_06295"
     CDS             4234..4983
                     /gene="pyrF"
                     /locus_tag="VGR03_06295"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV1993920.1"
                     /translation="MTTTFDAHPRSSLIVALDFDSLSSAVRFAKQVADLVGMFKIGNQ
                     LFTAAGPAAVKEVAALGPGIFLDLKFHDIPNTVAGAVLSSAAMTGVQLVNVHALGGKA
                     MLEAAVQAISAGVPMGADRPRLLAVTILTSMDQKTMKEVGIGGAPKLRVVKLAQLAKQ
                     VGVDGVVASVQEAKAIRKACGRDFLIVTPGVRPKESTVESKSEDQSRKATPTEAIRAG
                     ADFLVVGRPIIAAADPRAAAQAIVDEIAAAK"
     gene            5058..6968
                     /gene="selB"
                     /locus_tag="VGR03_06300"
     CDS             5058..6968
                     /gene="selB"
                     /locus_tag="VGR03_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEV1993921.1"
                     /translation="MSSPTKSVIVGTAGHIDHGKSTLVEALTGTHPDRLAEEKRRGIT
                     IDLGFAFLEENGVRFGFVDVPGHERFISNMLAGAAGIDVLLLVIAADESIKPQTREHF
                     DICRLLGVKCGVVALTKSDMVDSETLELVRMETEEFLRGSFLERAPIVPVSAKTGAGL
                     GELKKALSDAAANASAKDAARYFRLPIDRAFAMKGFGSVVTGTLISGSVGAGDEVELF
                     PRGERLRVRGVQSGGKAVERAMPGQRTAVNLAGIEHTALKRGMVLASPGKFRKTRRID
                     VRLELLPSARKMKQGSRVHFHAGTAETIAEVFFHGEKELPPGASALANLKFQDELLVL
                     PGDRFIVRQFSPVLTIGGGAVLDPLARRPMLRDTGRVKFLETIERGGSEDILVAMTER
                     ALLGLGYEEIVARTGWTEKEIHGALEKLHGTGRVRIVSAEPLVLVSGKLFEEVRKKIT
                     EKVEKFQKENPLLPGIAREDLRASLGKRVHSETFRAALEELAAQKKLDAQGDLVKKAG
                     SEIALLPEEAKAKEQIEAAFASASLAVPSVKEVLAKLVVESKRAEKLLQILLREKILV
                     RVSPELVFHRQALAQLKEQLSAYKKAKGDRISVSAFKELTGITRKYAIPLLEYLDRER
                     VTRRVGEERVIL"
     gene            7059..7271
                     /gene="tatA"
                     /locus_tag="VGR03_06305"
     CDS             7059..7271
                     /gene="tatA"
                     /locus_tag="VGR03_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEV1993922.1"
                     /translation="MGEFSIFHWLVVLAVILIFFGGKRIPEIMKGFGEGIRSFKDGMT
                     GTSQTPAPPAQAPAPPPADKPAEDKK"
     gene            7622..8275
                     /locus_tag="VGR03_06310"
     CDS             7622..8275
                     /locus_tag="VGR03_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993923.1"
                     /translation="MKFTWMKWSVLPVAVLMAGSVAMAQDQSSSGSQQPAPAAQPKPA
                     VAQHKENQQDRIANGVQSGQLTAGETSKLETKEAAINGETKADRAANGGKLTAAEKKQ
                     VNHQQNQVSKQIYADKHNANTAHYGNNKVGQRRENQQDRIAQGIKSGQMTAGEAAKAE
                     NQQKGINQQVAADRKANGGTLTASEKKQVNHEQNAASKNIYHKKHNAKTQPQAKPKP"
     gene            complement(8527..9102)
                     /locus_tag="VGR03_06315"
     CDS             complement(8527..9102)
                     /locus_tag="VGR03_06315"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEV1993924.1"
                     /translation="MKLILASASVRRAEILHDAGFSFTVLSSAVDETPYPGESAHDLV
                     QRLAFAKAELVAARAVGPAIVIGADTEVTLEGHIFGKPRSTDDARHMLEKLSGRTHAV
                     VTGVALIRLPDAERLTFAESTLVHFAMLSAEEISRYLATGEPHDKAGAYAIQGRASRF
                     IPRIEGCYFNVVGLPLARLQHALTALGWSED"
     gene            complement(9136..9666)
                     /locus_tag="VGR03_06320"
     CDS             complement(9136..9666)
                     /locus_tag="VGR03_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993925.1"
                     /translation="MPKKRRAFYAKYFKGRGTFLSLKLLPHLLAMHGTAVAPDEAETF
                     YKTGRISHDALELWQALGKHGALATLELRHACKMETQVGNKRFKKAMLELQALLIVTH
                     SGVEQETAAWASNRFELVSRAFPKQVAEASKLSAEEARTALAVQYRTLYPSASPVKVA
                     RLFGWTKAQAVSALAL"
     gene            complement(10295..11152)
                     /locus_tag="VGR03_06325"
     CDS             complement(10295..11152)
                     /locus_tag="VGR03_06325"
                     /inference="COORDINATES: protein motif:HMM:NF020584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoMIV family type II restriction endonuclease"
                     /protein_id="HEV1993926.1"
                     /translation="MKSVPKFRKTREAFHAHLCRDVLLINSDHVASNADGSNPASKEI
                     ALVLAKKLGASSGSRLPGQTSGDLFESICAEFVKTAFASLTHLRPGKWTVAQVSGPEM
                     EIAEFEQYSHLREIERLAQDHPQLKTILGGDYFIKPDVVVFRAPEEDSEINRDGLLVD
                     DTCTRLAPLRKKNNRFSTLHASISCKWTIRSDRTQNSRSEALNLIRNRKGHTPHIMVV
                     TAEPLPSRLASICLGTGDIDCVYHFALPELSETLSEMHYPDAQDMLKTLIEGRRLRDI
                     SDLPLDLAL"
     gene            complement(11166..12125)
                     /locus_tag="VGR03_06330"
     CDS             complement(11166..12125)
                     /locus_tag="VGR03_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEV1993927.1"
                     /translation="MTDFTCLELCAGAGGQAIGFERAGFSHVGLVEIEPDACATLRSN
                     RPSWRVIQEDIKKFDGSPYRGVDLLSAGLPCPPFSRAGKQLGHADERNLFPDALRILR
                     ECQPRVVMIENVRGILDPAFDYFRLELKETLAKLGYKSAWKLHNASDYGVPQLRPRAI
                     LVGVTLNCAAEFSWPRKSFAPPQPVSEVLKDLMLSRGWRGYRQWSKLARTIAPTLVGG
                     SKKHGGPDLGPTRARKAWAQLGVDGLGIANEPPGRDFSGMPKLTLEMTALLQGFPPDW
                     QFCGGKTSAYRQIGNAFPPPVAAAMGRNIQRCLMSKALARAAS"
     gene            complement(12227..>12753)
                     /locus_tag="VGR03_06335"
     CDS             complement(12227..>12753)
                     /locus_tag="VGR03_06335"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEV1993928.1"
                     /translation="ILANETGVVNTVDPVAGSYAIESLTDSIDSLASAYLSKIDKMGG
                     MLRAIEAGYVQGEIQKAAYDFQRAIEKKDQIVVGVNDFVAPSKGNDARTIPTLRIDAE
                     IERSQIARLNALRAKRDSFRAKSALAELQRRAATAENLLPAILAAVEAYATIGEISDA
                     LRRVFGEYQEAVVI"
BASE COUNT         2769 a         3574 c         3812 g         2598 t
ORIGIN      
        1 ttcgcgatgc ccgcggtcga tcagcacgca aagagagacg cgcttggggc ggccaagatc
       61 aaacagcccg ttcatcgccg cgcgaatggt gcggccggtg taaaggacgt catcgatcaa
      121 gaccaggtcc atattatcga cgccaaaaga aatgtccgag gagcgcaaca ccgcctgcgg
      181 ggcaactttg gaaaggtcat cgcggtacaa cgtaatgtcg agcacgccta cgggaacctc
      241 caggccatcg atgccacgca tggcctgggc aatccgctgg gcgagcggta cgccgcgtct
      301 cacgatcccg atcagcgcga ggtgtttcgt gccgcccgtt ctttccacga tttcgtgcgc
      361 cagccgctgg atcgtgcggt cgatttccgg cgcggacatc aattgccctt tttcgacgat
      421 acgcatggcc gggtaatcgt atcattggtg actgcggagt ggtcaatccg ctctgcttac
      481 acgccgctgt aaactggacg tgctagactc cgcggcggag tggggaggca tagctggcca
      541 tggctcgcag gacgaacccg atcggacacc gaactataag tgctctcgtt ctattggcgc
      601 tgatcttcgc atttccgccg ctgacaggcg cgcaaacaca gccccaggcc gcgacggcgc
      661 caaaagcgcc agcgaagccc aagctggtag tgctgctggt ggtggaccag atgcgcgggg
      721 actacgtgga caaattcctc ggccagtgga gaggtggcct taagcgcctc gtcgaagaag
      781 gagcgtggtt ccgcgatgcc gcctatccct acgcggccac ggagacctgc gtgggccacg
      841 caaccatttc aacgggagca cttcccgcga cgcacggcat ggtcgccaac gcctggtggg
      901 agcgcaagga acaaaagatg gtgacctgca cctccgaccc ggatcccagc gtaaaaaata
      961 ttggttatgc cggcgccacc ccaaaaggcg cagacacagc ttggcgcatg gccgtgccct
     1021 cgtttgccga agaattgaaa tttcagacca gcggcgcaac acgcatcgtg acgttttccc
     1081 tgaaggcccg cgcggccatt accatggctg ggcacaaagc cgatgccgcc acttggttcg
     1141 acagcggcgc gtgggtcacc tcgaccgcct acggcacgct gcctttcatg gaagagcaag
     1201 ccaagtcgca tcaggcgaaa gccgacttcg gcaagacctg gtcgctttcc ctcccggaaa
     1261 gcgcctattg gtacggtgaa aaagctctgg gcgccgttcc tcccgatggt tgggatctca
     1321 cgtttcccca cccgctgcgc ggaaaatcga gcgtcggtga accggacagc gccttctacg
     1381 aacaatgggc ctcgagccct tacgccgata cagccctgac cgaacttgcc gaaaaagccg
     1441 tcgacaccct gaatctcggc aaagccgccg gaaccgattt tctcgccgtc agctactctt
     1501 cagtggatta cgtcggccac gaattcggcc ctcgcagccg cgaaatccag gacatcctag
     1561 tgaggctgga caaagacctc ggcaacctct tcgcgcactt ggatcagaag gtcggccgcg
     1621 gaaattacgt cgtcgctctg agcgccgacc acggcgtggt gccgattccc gaagacatgc
     1681 aaaagaccgg cgctgacgcc ggcgtccttc atttgctgga gttgcaggaa cgaatcgaaa
     1741 aagccctcga gcctttcaaa tacacaaagc ccgccattgc gcgcatcacc ggcagcgaca
     1801 tttattttgc gcccggcgtc tacgacaagc tccagcagga tcgcgctggg ttgcaggccg
     1861 ttctggacgt agcactggcg cagcccggcg tcgccgccgt ctatcgcgcc gaagaattgc
     1921 gcgaccgccc cgccacgcaa agcccctcgc accgcgccat ggcaaacggt tatttcccgg
     1981 gccgcagcgg cgatctattc atcctgccaa agccctactg gctcatggac gggacgcctc
     2041 ttgggaagcc gcgctcctac ggcaccggcc acggcacgcc atacaactac gaccagcacg
     2101 tcccagttct actgatgggc ttcggcatcc aacccggcca atacttccag cccatcacgc
     2161 ccgccgacat cgccccaacc ttcgccgccc tctgcggcat caccctctcc tcccgcgacg
     2221 gccacgtcct cgccgaagct ctgaaaaaac cagcagagca ccgcgccccg gctcagcccg
     2281 cgcacccaaa atccgcaccc gcgtcgcctc caagcacaaa tccctgatgt aaccatctgc
     2341 cttcagaaat gttgtgctgc gtcctcaaat ctgacgcccc gggtcctacg tagtcggtca
     2401 gtttctgagt agcgccttag tcgggactag gcccattgca gtctaaggaa tagcgacaac
     2461 aaggtgttag agtcgtagaa tctcggaatg ccaagcgtct gggtaaaatg gcggctttcg
     2521 ccgtttgcat attctgaaat aactatcaac gacgtattgg gtgaagtaag ccttgccttg
     2581 gatgaaccgc tgtgcgagtc aaggggtgaa cttcacagtg ggaaaacgta cattgtaaac
     2641 acgaagcgag ggctgtatgt tctctgccgt cggtgctgta agacgctcgg cttaccgctc
     2701 cacagctaaa ttccgataag tcggggaccg cctggctatc agattttgcg cggagtagaa
     2761 acgtcttcgt gttatccttt tgcacgaagt gagctccgct ggcgctgtcg acctcagcgt
     2821 aaaaattggc gcgttgcgcc tgcggaatcc tattctcgct gccagtggaa ccttcggtta
     2881 cggcctcgga ttcgcccacc ttgttgacct gaaccgcctc ggtggtttcg tcaccaaggg
     2941 cctttcccgc gaacccatcg aaggcgcccc cgccccccgg ctctacccca ccccctccgg
     3001 tatgctcaat gccgtggggc tccaaaatgt cggggtccgg gcctttgtag ccgagaaact
     3061 cccggtactg cgaaaattcg atacggcagt catcgccaac gtattcggct gcactcttga
     3121 cgactacatc caggtgatcc gggttctgga ggacgccgag ggcctcgccg gctacgaact
     3181 caatatctcc tgccccaacg tcaaaaaggg agggatgcag ttcggcagcg atcccatcca
     3241 agtagccgag gtcgtggcgg ccgcccggaa ggctgccacg aagagacccc tctgggtcaa
     3301 gctctcccct cttgttacag acattggact tatcgcccgg gccgccgagg aggccggagc
     3361 cgacgccctg accgtggcaa acacctatcc cgcaatggcc atcgatttcc gctctgggaa
     3421 gtcccgcctc ggcaacccaa cgggaggcct ttcgggtcca gccattaaac ccattacttt
     3481 gaggctcgtc tgggaaacga agaaggccgt taagacacca attatagggc tgggcggcat
     3541 cgagtcggtc gaagacgtgc ttgattacct tgccgtaggc gcttcggcgg tacaggtcgg
     3601 gacggccagc tttactgatc cgagggccag tcagcgactt gcaggcaccc ttacgaaggt
     3661 cctattacga gctaaagcgt ttactttgag tgagataaga gacaaattcc tggccgaaaa
     3721 tggttgacat aatgactcct tttcgctaca cttttctagt tcttagtagc acaaagaaca
     3781 cactatgaga catcgacttt tcatgagctg ggctgcactg cgctgggtac ttctcatgtt
     3841 cctcatcgct ccgggcatgg aagcacccct cgtggcgcat ttgaatcaac ccgtgaagcc
     3901 gatcaagacc tgggtgggta attccagttg gtacggccca ggctttaacg ggcgcaagac
     3961 tgccaacgga gagcgcttcg actccgaagc tctcacggcg gctcatccca acctgccttt
     4021 tggctcgttg gtacgtgtag tgaacccccg caacggcaaa tttgaactgg ttcgcatcaa
     4081 tgaccggggt ccctatcaag aagggcgcga aattgatgta tcctatcgtg tcgcccggaa
     4141 aatcggcttg atccattcgg gcgtgagcca ggttcggctc gagctcatgg aactacccgg
     4201 gaaacactga cctcgaagga gtcggggtcg cttatgacca cgaccttcga cgcccatccc
     4261 cgcagcagcc tgatcgtcgc tcttgatttc gattctctat cctccgccgt tagattcgca
     4321 aagcaggttg ccgatctcgt agggatgttc aagatcggca accaactctt taccgccgca
     4381 ggccctgctg ccgtcaaaga agtagcagcg ctcggcccag gtatttttct cgatctgaaa
     4441 ttccacgaca ttcccaacac cgttgctggc gccgtgcttt cctcggcggc catgacggga
     4501 gtgcagctcg tcaacgtgca cgcgctcggc ggcaaagcca tgctcgaggc cgcggtgcag
     4561 gccatcagcg ctggcgttcc gatgggagcc gatcggcccc gcctcttggc ggtgactatc
     4621 ctgacgagca tggatcagaa gactatgaag gaagtgggga tcggcggcgc tcccaaactc
     4681 cgtgtcgtga aactggcgca acttgccaag caggttggcg tcgacggcgt cgtcgcttcg
     4741 gtgcaggagg ccaaggcgat tcgcaaggct tgtggccgcg atttcctgat cgtgacgccc
     4801 ggggttcgtc cgaaagagtc aacagtggaa tccaaatcag aagaccaatc ccggaaagct
     4861 acacctaccg aagcgattcg cgccggcgcc gatttcttgg tagttggcag gccgatcatt
     4921 gccgcggccg atccgcgagc cgcggcgcag gcgattgtcg acgaaatcgc tgccgcgaag
     4981 tagttttcgc agtttgaccc ctgattcttt cctccagtag catccaaagc ggcgcgaagc
     5041 agcccacacc ttcattcatg tctagtccga cgaagagcgt aattgttggc acagccgggc
     5101 acatcgacca cggcaaatcg acgctggtcg aagcgctgac cggcacgcac ccggatcgtc
     5161 ttgccgaaga gaagcgccgc ggaatcacta ttgatttggg ttttgctttc ctggaagaaa
     5221 atggcgtgcg cttcggtttt gtagacgtac ccgggcacga gcggtttatc agcaacatgc
     5281 tggcgggcgc tgcgggcatc gatgtgctgc ttttggtgat cgcggcggac gaatccataa
     5341 agccacagac gcgcgagcat ttcgatattt gccggttgct gggagtgaaa tgcggcgtgg
     5401 tggcgctgac gaagagcgat atggtggaca gcgaaactct ggaactggtg cgaatggaaa
     5461 cggaagaatt cctgcgagga tcgttccttg aacgcgcgcc gattgtgcct gtgagcgcga
     5521 aaactggagc cggactcggc gagctgaaaa aagccttgag cgacgcggct gccaacgcgt
     5581 ccgccaaaga tgcagcgcgc tatttccggc tgcccatcga tcgcgcgttt gcgatgaaag
     5641 gatttggttc ggtggtaacg gggacgctga tttccggaag cgttggcgct ggtgatgaag
     5701 tggaattgtt tccacgcggg gagcggctgc gcgtgcgcgg cgtacagtcc ggcgggaagg
     5761 ccgtggagcg cgccatgccg ggacagcgca cggcggtgaa tcttgccgga atcgaacaca
     5821 ctgcgttgaa gcgtggcatg gtgctggcgt cgccgggaaa attccgcaag acgcgccgca
     5881 tcgatgtccg gcttgagttg ctgccgtccg ccagaaaaat gaagcaaggc tcgcgcgtgc
     5941 actttcacgc cggaacggcg gagaccatcg ccgaagtatt ttttcacgga gagaaagaac
     6001 tgcctcccgg cgcgagcgcg cttgcgaact tgaaatttca agatgaattg ctggtgctgc
     6061 cgggcgatcg cttcatcgtg cggcaattct cgccggtgtt gacaattggc ggaggcgccg
     6121 tgctggaccc gctggcgcgg cggccgatgc tgcgggatac gggacgagtg aagtttctcg
     6181 aaacaatcga gcgtggaggc agcgaggata ttctggtggc gatgacggag cgcgcgctgc
     6241 tggggctcgg gtacgaagag atcgtggcgc gaacgggctg gacggaaaag gaaatccatg
     6301 gagctctaga aaaacttcac ggaacgggac gcgtcagaat tgtatcggcc gaaccgctgg
     6361 tgctggtctc cggaaaattg tttgaagaag tacgcaagaa aattactgaa aaagtcgaaa
     6421 aatttcagaa ggagaatccc ttgctgccgg gaatcgcgcg cgaggatttg cgcgccagcc
     6481 tgggaaagcg cgtgcacagc gaaacattcc gcgccgcgct ggaagaattg gccgcgcaga
     6541 agaagctcga cgcgcagggc gacttggtaa aaaaggcagg atccgaaatc gccctgcttc
     6601 ccgaagaagc caaggccaag gaacagatcg aagcggcgtt tgcttctgcg agtctggcgg
     6661 tgccatcggt gaaagaggtg ctagcgaaac ttgtcgtcga atccaagcgc gccgagaaac
     6721 tcctgcagat tctcctgaga gaaaaaatcc tggtccgcgt ctcgccggaa ctggtttttc
     6781 accgtcaggc gctggctcaa ttgaaggagc agctttccgc ttacaaaaag gccaagggcg
     6841 accggatttc cgtttccgcc ttcaaggaac tcacggggat tacacgtaag tacgccatcc
     6901 ccctactcga atatctcgac cgtgagcgag tgacgcgccg ggtgggggaa gagcgtgtta
     6961 tcctgtaggt gcaacaagcg gcagagactt gccgcgcagg gctacctgcg ctagggttta
     7021 aagagcgcgc cgccaagtgg ggcgcgtgga ggcgttttat gggtgaattt agtatttttc
     7081 attggcttgt ggttctggcc gttattctaa ttttctttgg cggcaagcgc atcccggaaa
     7141 ttatgaaggg atttggcgaa ggcatccgca gcttcaagga cggcatgacg ggtacttcgc
     7201 aaaccccggc gccgccggca caggcgcctg ctcccccacc cgcagacaag cctgccgaag
     7261 acaagaagta gcgcagaggc gcacaaaggt ttcctaccgt tttgagatgg ggcggcccgt
     7321 gtggacgccc ttttcatttt caaagaatga tgtatggagt gcgggaggcc ttgctcccac
     7381 tttttcgcct gcggtccgtc agcaacggca aaaacggcag ctggggcccg acaggagtcg
     7441 gggtgaactg ccgcactcca caggttctcg attgtgtcca aagagtagcg gaaaaccctt
     7501 atccaacgac gaaaatctgc acttccggcc agtagcctcg gcacaacttt gcgttcagca
     7561 tccggtatta cagggtgacc cgggaatcga aatcttcgaa atgattcatg aggagagaaa
     7621 catgaaattc acttggatga agtggagcgt cttgcccgtt gcggtgctga tggccggcag
     7681 cgtggcgatg gcgcaggatc agtcgtcttc gggctcgcag cagccagcac cggcggcaca
     7741 gcccaagccg gcggtggcgc agcacaaaga aaaccaacag gaccgcatcg ccaatggcgt
     7801 acagagcggc caactgacag ccggtgaaac ttccaagctg gaaaccaaag aagcggccat
     7861 caacggcgaa accaaggcgg accgcgcagc caacggcggc aaactgacag ccgccgaaaa
     7921 gaaacaggtc aaccatcagc agaaccaagt ttccaagcag atctacgccg ataagcacaa
     7981 cgccaataca gcgcattacg gcaacaacaa agtcgggcag cgccgcgaaa accagcagga
     8041 ccgcatcgcc cagggcatca agagcgggca gatgacggcc ggcgaagctg ccaaagccga
     8101 gaaccaacag aagggcatca accagcaggt ggccgcggac cgcaaagcca acggcggaac
     8161 gctgacggct tccgaaaaga agcaagtcaa ccacgaacag aacgccgcgt cgaagaacat
     8221 ctatcacaag aagcacaacg ccaagacgca gccgcaagcc aagcccaagc cgtaagtttt
     8281 ctccaccccc tgaagtaaga gaggcgtccc ggtgagaacc gggacgcctc tttatttatg
     8341 gacgcaggat ttcgctttgg tgaggcggca actgagatcc ttctccgatc ctcatcggat
     8401 gggttcggga tgacagatca tgcttacttt gcattgagtg gatgaatcat ggagacggcg
     8461 agaatgtctc acctaacgcg atctgcggga tcacaatacg cgagcgtgtg cggttggtgg
     8521 ctacgattag tcttcggacc agccgagggc ggtgagggcg tgctggagtc gtgcgagggg
     8581 caggccgacg acgttgaagt agcagccttc gatgcgcgga atgaaccggc tggcgcggcc
     8641 ctggatggcg taggcgccag ccttatcgtg gggttcgccg gtcgccaggt agcgggagat
     8701 ttcttcggcg gagagcatgg cgaagtgcac tagcgtggac tcggcaaagg tgagccgctc
     8761 ggcgtcggga aggcggatga gggcgacgcc ggtgacgacg gcgtgtgtgc ggccggaaag
     8821 tttttcgagc atatggcggg catcgtcagt ggagcggggc ttgccgaaga tgtggccttc
     8881 gagagtgacc tcggtgtcgg cgccgatgac gatggccggg ccaacggcgc gtgcggcgac
     8941 gagttcggct ttggcgaacg ctagacgctg aacgaggtcg tgggcggatt cgccagggta
     9001 aggtgtttcg tcgacggcag aggagagcac ggtgaacgag aaaccagcgt cgtgaaggat
     9061 ttcggcgcga cggacggaag cggaggcaag gatgagtttc atggtgaatc ctgggatttc
     9121 tggaagggaa ggatactata gcgcaagtgc gctgacagcc tgtgctttgg tccagccgaa
     9181 gagtcgggcg actttaactg gcgacgcgga ggggtagaga gtccgatatt gcacggccag
     9241 cgcggtgcgg gcttcttcag cggaaagttt gctggcttct gccacttgtt tgggaaaggc
     9301 tcgagaaact aattcaaagc ggttggaggc ccaggctgcg gtttcctgct ccacgccgga
     9361 atgcgtgacg atcaacaggg cttgcagttc gagcatggct ttcttgaagc gcttattgcc
     9421 cacttgcgtt tccatcttgc aggcgtggcg aagttcgagc gtcgcgagcg cgccgtgctt
     9481 gcctaacgcc tgccacagtt ccagtgcatc atgcgagatg cgccctgttt tgtaaaaggt
     9541 ttcagcctca tccggcgcta ctgccgttcc gtgcatcgct agcaggtgcg gaaggagttt
     9601 aagcgagaga aacgtgccgc gacccttgaa gtatttcgcg tagaaggcgc ggcgcttttt
     9661 cggcaattcg tctttccatt tccagatgag ctgcgcgtat ttgtcccaac gggcgttggt
     9721 ccgcctcgcc gcggcatagt agagcgaagg cagcgagacg tttttcaccg ggaaaaggac
     9781 acagaatccg gcgtgatcga tgaaacgcac ggcttctgaa ggcatggaca cgcggcgtgg
     9841 ccagggaaat tgggagattt ctgtcgaaac caagttagcc accccgcaga aacgttttta
     9901 tccgttgctt caccttttcc acgtccctga gctgacactc ccaaactacg agacttctcc
     9961 atccgagata tctcaatttg gcagcattct tgaggtctcg ccgtttgttt ccttcgagct
    10021 ttggaaccca gtagttctgt ttggacttag ggatccgcgc aatcgtacag cggccgtgga
    10081 aatgccaaaa gcatccgtga agaaacaagg cttttcgttt tatcggaaag acaaagtccg
    10141 gacggccggg caaattcttg gtgtgcaagc gatacttgta ccgaagggat cggagaatct
    10201 ggctgagcat tctttccggg acagtattct tacttcgaat acgccgcatg ttttcgcttc
    10261 tctctttggg agagagtttg tccatacgag ttggctaaag cgccagatcc aggggcaggt
    10321 cggagatatc tcgaaggcgc cttccttcga tcagcgtttt cagcatatct tgagcgtccg
    10381 gatagtgcat ttcggaaagg gtctccgaca gttcggggag cgcaaagtga tacacgcaat
    10441 cgatatctcc tgttcccaaa cagattgaag ccagtcggct cggaagcggc tcagccgtaa
    10501 caaccatgat gtgaggtgta tgacctttgc gatttcgaat gaggttcagt gcctcggaac
    10561 gggaattttg agtccggtcg cttcggatcg tccatttgca ggaaatgctg gcgtgaagtg
    10621 tcgaaaaacg attgttcttc ttgcgcagcg gtgcaaggcg tgtgcatgtg tcgtcgacca
    10681 atagtccatc gcggtttatt tctgaatctt cctcgggcgc gcgaaacacc acgacatctg
    10741 gcttgataaa gtagtctcct cccaggattg tcttgagctg ggggtggtct tgtgccaagc
    10801 gctcaatttc gcgcaaatgc gaatattgtt cgaactccgc tatctccatt tcgggtccgg
    10861 agacttgagc tacggtccat tttcccgggc gaagatgggt caggcttgcg aacgctgttt
    10921 ttacgaactc cgcacagatg ctctcaaaga ggtctccaga agtctggcct ggtagtctgc
    10981 ttcctgaaga agccccaagc ttcttcgcca gaacgagggc gatctctttg cttgcaggat
    11041 tactgccatc ggcattcgac gccacgtgat ccgagttgat gaggagtaca tctcggcaaa
    11101 ggtgcgcgtg gaaggcttct cgcgttttac gaaacttagg cactgacttc atgagcatcc
    11161 aagaatcaag aagctgccct tgccagagct ttcgacatca ggcatctctg aatatttctt
    11221 cccattgcag cagcgacggg tgggggaaat gcattgccga tctgacgata ggcacttgtt
    11281 tttccgccac agaattgcca gtccggtgga aatccttgta aaagggctgt catctccaat
    11341 gttaacttag gcatccctga aaaatcgcgg cccgggggtt catttgcgat gcccaagcca
    11401 tccacgccga gctgtgccca tgccttgcga gctctcgtcg ggccgagatc aggccctccg
    11461 tgtttcttag atccacctac aagagtcgga gcgatggtgc gcgctaattt ggaccattgc
    11521 cggtagcctc tccagcctcg ggagagcatc aagtctttca atacttcact aacgggctga
    11581 ggcggcgcaa aagatttcct gggccaggaa aactccgctg cacaattcag agttacgcca
    11641 acaagaattg cacgggggcg caactgaggc acgccgtaat cggaagcgtt gtgcagcttc
    11701 cacgccgatt tgtaccctag ctttgcgagc gtttccttca gttctagtct gaaataatcg
    11761 aaagccggat ctagaattcc acgcacgttt tctatcatca caacgcgtgg ctggcattct
    11821 ctaagaattc tcaatgcgtc cggaaacaga tttcgctcgt cagcatgtcc tagttgttta
    11881 ccggctcgcg agaagggcgg gcacggcagg cctgcggaca agagatccac gccacggtac
    11941 ggcgatccgt cgaatttctt tatgtcttct tgaattactc tccatgaggg gcggttcgac
    12001 cgcaaagttg cgcaggcgtc aggttcgatc tcaactagcc cgacgtgaga aaaaccggcc
    12061 ctctcaaatc caattgcttg gccaccggcg ccagcacaga gttccaggca agtaaagtca
    12121 gtcatacgaa gccgccgttt gctcggtcac gtggatgagt cacgaaagta taccatcgcg
    12181 cggtcctgtg ccgaatctag atgccgcggg aactgacttt cctgagtcag atgacgacag
    12241 cttcctggta ttcgccaaag acgcggcgga gggcgtcgga gatttcgccg atggtggcgt
    12301 aggcttcgac ggcggcgagg atggcgggga ggagattttc tgcggtggcg gcgcggcgtt
    12361 ggagttcggc gagggcggat ttggcgcgga aggagtcgcg cttggcgcgg agagcgttca
    12421 agcgggcgat ttgggagcgc tcgatttcgg cgtcgatgcg cagcgtggga atggtgcgcg
    12481 cgtcatttcc tttcgatggt gccacgaagt cgttcacgcc gacgacgatt tgatcttttt
    12541 tctcgatggc gcgctggaaa tcgtaagcgg ctttctggat ttctccctgg acgtatccgg
    12601 cttcgatggc gcgcaacatt ccgcccatct tgtcgatttt ggaaagatag gccgacgcaa
    12661 gcgaatcgat gctgtcggtg agggattcga tggcgtagga gccggcgacg gggtcgacgg
    12721 tgttgacgac gccggtttcg ttggcgagga ttt
//
LOCUS       DASYVO010000188         2730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_203555, whole genome shotgun sequence.
ACCESSION   DASYVO010000188 DASYVO010000000
VERSION     DASYVO010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203555"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1717)
                     /locus_tag="VGR03_06340"
     CDS             complement(146..1717)
                     /locus_tag="VGR03_06340"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEV1993929.1"
                     /translation="MKKRAFPVTLASVPIDRTQLPEDASVLQQMVLDLIAQLDAEQSK
                     RIKTEQLLRQLLAARTGRKSEQLSREQLALFAEELKAQGVKLPEEAGVDDDNDHVPPS
                     PGGAAAKPHGRRPMPEQLKRERIVHDLAEAEKHCHACAQDLRPIGEESSERYEYIPAQ
                     VIVIEEVCKKYACDCTVKTATKPSQPIEKSTAGASLLTQVIVSKYADHLPLHRQSKML
                     RRVGVDLSDKTMCGWMGQCADLLDPLYERLKSFVLESKVVGTDDTQVKVLDRSLPHAR
                     KGRMWPYAGDRDHPAVVYDYTATRERAGPEKFLGNYRGHLQADAYVAYDGLFDKAERG
                     LIEVGCWAHARRHFHDALESDRSHMGAVLLLIAELYAVEKIARQGDLRGEDLRLARQH
                     GAKPVLDRLHAYLLEIQPELLPKSEAGKAVAYTLKNWAALTRYAEDGDLAIDNNATER
                     AIRCIAVGRNNWTFFGSDNGGKTAAVLRSFVTSCELVKIDPFAWFRDVLSRIADHPIT
                     QLDDLLPHRWAIAQR"
     gene            complement(1744..2109)
                     /gene="tnpB"
                     /locus_tag="VGR03_06345"
     CDS             complement(1744..2109)
                     /gene="tnpB"
                     /locus_tag="VGR03_06345"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1993930.1"
                     /translation="MMHLPASVRVYLCACPCDMRKSFDGLHALVTDCLKLDAFAGHLF
                     VFANRRRDRVKILYWDRDGFAVWAKRLEQGTYAMPFEEGEQPRREITAQELGALLSGI
                     DLSQAKRRKRYQRRITEAA"
     gene            complement(2106..2435)
                     /locus_tag="VGR03_06350"
     CDS             complement(2106..2435)
                     /locus_tag="VGR03_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1993931.1"
                     /translation="MKLSKRGGRYSDRHEHWKAVIAEQKRSGQTVGSFCHQRGVTQQS
                     FYQWRKRLGAAQAPVRFALVETGRPEARERAAVELVLRSGHRLRIAAGVDGTTLRTVL
                     EVLEARA"
BASE COUNT          496 a          899 c          741 g          594 t
ORIGIN      
        1 ctgtgccgtt atcatgccgg tgaatagatc gcgggaatcg cgcggctccg tgcatcttca
       61 aagtaacgtt aactgtaagc gcccggtgaa cgccgggact ctattgacgg cgggaccgca
      121 atgagagtag tgacgcaagg agaggttagc gctgggcgat cgcccaacga tggggcagaa
      181 ggtcatcgag ctgggtgatg ggatggtcgg cgatgcggga gagcacgtcg cggaaccagg
      241 cgaacggatc gatcttcacc agttcgcagg aagtgacaaa gctgcggagc accgccgcgg
      301 tcttcccgcc gttgtcgcta ccgaagaacg tccagttgtt gcggccgacc gcgatgcagc
      361 ggatcgcacg ctcggtggcg ttgttgtcga tggccagatc gccatcctcg gcgtatcgcg
      421 tcaacgcggc ccagttcttg agagtatacg ccacggcctt gcccgcctcg ctcttgggca
      481 acaactccgg ttgaatctcc aataagtacg cgtgcaaccg gtccagcacg ggcttggcgc
      541 cgtgttgccg cgccagccgc agatcctcgc cgcgcaaatc tccttggcgg gcgatcttct
      601 caaccgcata aagctccgcg atcaatagca acacggcgcc catgtgcgaa cgatcgctct
      661 ccagagcgtc gtgaaaatga cggcgcgcat gcgcccagca gcccacttcg atcaaaccgc
      721 gttccgcttt gtcgaacaag ccgtcatagg ccacgtaggc gtcggcctgc aaatgcccgc
      781 gatagtttcc cagaaacttc tccggccccg ccctctcacg cgttgccgta tagtcgtaaa
      841 ccaccgctgg atgatcgcga tccccggcat acggccacat ccggcccttg cgcgcatgcg
      901 gcaggctccg gtccagaact ttcacctgcg tgtcgtcggt tcccaccact ttggattcca
      961 acacgaagct cttcagccgt tcgtacagcg gatcgagcag atccgcgcat tgccccatcc
     1021 atccacacat cgttttatcg gacagatcca cgcctacgcg ccgcaacatt ttcgattgcc
     1081 ggtgtagcgg caggtgatcg gcatacttgg acacgatcac ttgcgtcagc aggctcgcgc
     1141 ccgccgtgct tttctcgatc ggctgcgacg gcttcgttgc cgtcttcacc gtgcagtcgc
     1201 acgcgtactt cttgcacacc tcttcgatca cgatcacctg tgccggaatg tattcgtaac
     1261 gctcgctgga ctcttcgcct atcggccgta ggtcttgcgc gcaggcatgg cagtgctttt
     1321 ccgcttcggc gagatcgtgc acaatgcgct cgcgcttcaa ctgttccggc atcggccggc
     1381 ggccatgcgg cttcgctgcg gcgcctcccg gggacggcgg aacgtggtcg ttatcgtcgt
     1441 ccacgcccgc ctcttccggg agcttcacgc cttgggcttt cagctcctcc gcgaatagcg
     1501 ctaactgctc gcgcgaaagc tgttcgctct tacgtcccgt cctggccgcc agcaactgcc
     1561 gcagtaactg ttccgtcttg atccgcttcg attgttcggc gtccagttgc gcgatcaaat
     1621 cgagcaccat ctgttgcaac acgctggcgt cttcgggaag ctgtgtgcgg tcgatcggca
     1681 ctgaggcaag tgtaaccgga aacgcacgtt tcttcaagag aaaacatgca ctatcgccaa
     1741 tgtttatgcg gcttccgtga ttcttctctg gtatcgcttc cgccgcttcg cttgactcag
     1801 atcgatgccg cttaacagcg cccccaactc ctgcgccgtg atctcccgcc gcggctgttc
     1861 gccttcctcg aacggcatcg catacgtgcc ctgttccaat cgcttcgccc ataccgcgaa
     1921 tccgtcccgg tcccaataca ggatcttcac ccggtcccgc ctccggttgg cgaacacgaa
     1981 cagatgcccc gcgaacgcat ccagcttcag gcaatccgtc accagcgcgt gcaacccatc
     2041 aaaactcttc cgcatgtcgc atgggcacgc gcacaggtac acccgcacac tcgccggtaa
     2101 gtgcatcatg cgcgggcttc gagaacttcc agcaccgtgc gtagcgttgt cccgtccacg
     2161 cccgctgcga tgcgcaagcg atgtcccgaa cgcagcacta actccaccgc agccctttcc
     2221 cgcgcctctg gcctgcccgt ctctaccagt gcgaatcgca ccggagcctg cgccgctccc
     2281 agccgcttcc gccactgata gaaggactgc tgcgttacgc cgcgctgatg gcagaagctt
     2341 cccaccgttt gtccacttcg cttctgctcg gcgatcaccg ccttccagtg ctcgtgccga
     2401 tccgaatatc tgccacctct cttggaaagt ttcatcctcc ccagtctgct cgaatccttc
     2461 aatcttgaac agtggggttc accgggcgct tacgattaat cagtggttgg tggggaatgg
     2521 aattacgttg ctgaaagatg atgggttcaa tcattatcgg gttgcccaag ccctgttgcc
     2581 gaggctgacc ctagctacgc tggctacaac ggaccttgac cgttttgaag cgctcttcac
     2641 tcgtgtgaat gaaggactga gctgaattgt gattctcgcc tcgttgcgac ggcagatgct
     2701 caggaggttt atcttgtgtc acgaaagttc
//
LOCUS       DASYVO010000189        11029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_73984, whole genome shotgun sequence.
ACCESSION   DASYVO010000189 DASYVO010000000
VERSION     DASYVO010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 11029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11029
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73984"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VGR03_06355"
     CDS             complement(<1..291)
                     /locus_tag="VGR03_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993932.1"
                     /translation="MELKTLRKRLPTAALNHFFVRIGGAALLAVLILSPAIQAQQSGG
                     APPNEAETVAELLKRVQHLEARLEQLEEERAKNATPTTPTRAEAAQLTVTPAP"
     gene            complement(1397..1473)
                     /locus_tag="VGR03_06360"
     tRNA            complement(1397..1473)
                     /locus_tag="VGR03_06360"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1437..1439),aa:Pro,seq:tgg)
     gene            1891..2733
                     /locus_tag="VGR03_06365"
     CDS             1891..2733
                     /locus_tag="VGR03_06365"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1993933.1"
                     /translation="MRQQLDAGQFYGRTLRRRSFGDLTLADVEYRAHSHLPRHSHERA
                     YFCLIRRGTYVESYSRRTRVCAPMMLVFHPPAEPHSEAFDDEPVVSFNVELGAEWLCR
                     LRELGGTFDQPIEFHAGRTVALGLRLFKEFARGNIDSDLSIESITLEILAAYISKRPT
                     AAYSIKPDWLRRASETLDACLDQSLSLGSVAKSVGVHPVYFALMFRRFNGCSVGEYVR
                     RRRIEYARRMLANPEIPLTQVALGAGFADQSHFTRTYKRFTGRTPSQDRTFLRFKTPP
                     DPGS"
     gene            2776..3831
                     /locus_tag="VGR03_06370"
     CDS             2776..3831
                     /locus_tag="VGR03_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1993934.1"
                     /translation="MRWAMLKRFVFLIFILQLADIAFAQQGPAFAAPSRLVDLGGFQV
                     NIYCVGHGRPPVVFLHGLGDYSFDWALVQPALAEHTEACAYDRPGQAWSEPGPPPRGI
                     STSAHELHLLLKRSNVKGPYILVGHSWGGLIARMYAHEYPKEIAGMVLVDSTHEDEYL
                     WISGKILRPRYMTDEEWADLRRPLKAPSPAPSSDTAKQAPAPQQPIVTQLPSPYDKLP
                     PDAQRLRLWAMSLPWSKKRNEGGDTLDLRQDFIATYKVRIQSGHPLGSIPLIVLSKTP
                     GIEDDDDYTPDQLKWNRDLQDQLATLSTNSQHLVADHSGHHIQLEEPDIVIASILRVV
                     DAVRDKRPLRNEPISAH"
     gene            complement(4316..5344)
                     /locus_tag="VGR03_06375"
     CDS             complement(4316..5344)
                     /locus_tag="VGR03_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEV1993935.1"
                     /translation="MMRLPLFEFRTPRTLEEAARILHGEGASAMPLAGGTDLLPNMKR
                     RQQVPRMLMSLRYVEGLRQVQLGELASRLGACLTLAEIAGDPRFRNGWTALWQAAAQV
                     ATPQIRNMATLGGNLCLDTRCNYYDQSYEWRKAINFCMKKDGTTCWVAPGSPKCMAVS
                     STDTAPALIALGARVRLVSRSEEREVPLSELYNNDGIHYIRRRPDEILAEVLLDSLHG
                     WKSTYWKLRRRGSFDFPVLSVAAAARLSVEGVVEEARIVVGSAACLPLVAAEAAKSLL
                     GRSLNQESIAEAAALAARIAKPLDNTDFDMSWRKKVTAEFVTCALRELRGDDVHTERE
                     RLTRHPLL"
     gene            complement(5341..7857)
                     /locus_tag="VGR03_06380"
     CDS             complement(5341..7857)
                     /locus_tag="VGR03_06380"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV1993936.1"
                     /translation="MPNQKDMPDRPARDSKPNGDELRVIGVPRRRVDARAKVTGQTRF
                     ADDIFLPRMAHCKLLRSPHPHARIRKIDASRALAHPGVYLVLTGKDVSIEYGILPISQ
                     DEQALCVDHVRHVGDPVAAVIAREELTAFEALDLIDVEYELLTTISDPEEALATPEPR
                     IHDYGEEGNIHKRVALDFGDVEKGFAEADEIFEDTFFYQGSTHLPIEQHAAVAIKDPE
                     GKLTIWSSTQTPHYVHRALAKVLEMPAAHIRVIATPNGGGFGGKSDPFQHEIVVSKAA
                     LVLDRPVKICLTREEVFYCHRGRHPVLMKFKTGVKKDGTITGMHLQTLIDGGAYGSYG
                     VASTFYTGALQTTTYHVPRYRFQGCRTFTNKPPCGPKRGHGTVQPRFGQEVQMDKIAE
                     KLKIDPAELRLRIVESPNTVTANFLRLGTVNLSECIRRVVQVSGWNEKFRKLPEGRGV
                     GLACSAYLTGAGLPIYWNKMPHSGVQLKLDRGGGVTIFCGATEIGQGSDDMLAAIVAE
                     VLGVTTFDIRVYTGDTDLGPVDLGSYSSRVTLMAGNAAIQAAERAKAMLAEAVAQRLE
                     VPKERLRFADNRVFDSAAPQSGVAFQEAVCLAEARFGTIGTVGSYTPPKSPALYKGGG
                     VGPSPTYSYSAAVVEVEVNPTTGWVTVPRIWIAHDIGRALNPPLARGQVEGSVYMGLG
                     EALMEEQEFRRLPKKLSHALVHKFPSILEYKSPTSLDMPDVVTVLIEEPDPRGPFGAK
                     EVGQGPLLPIMPAVANAVYDAIGVRIDEVPITPEKIVKALAEKKAGRTPRFGPSHFPH
                     VDWPESLRVPPPWEGGDGNAVNDVPRRTKPRPKKEEVLSS"
     gene            complement(7901..8422)
                     /locus_tag="VGR03_06385"
     CDS             complement(7901..8422)
                     /locus_tag="VGR03_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV1993937.1"
                     /translation="MNLITLPAASQKTAHIRMTLNGEEVDVSFAPYKTLLEVLREDLG
                     HTGTKHGCELGECGACAVLLDGKPVLSCLVLALECDRRDVLTVEGLGADGNLHPLQDA
                     FADLGAAQCGYCTPGILVTAKALLDHNPHPSRDQIREALSGTLCRCTGYLQIFEAVEA
                     AMIKIAEQKESAA"
     gene            complement(8439..9158)
                     /locus_tag="VGR03_06390"
     CDS             complement(8439..9158)
                     /locus_tag="VGR03_06390"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV1993938.1"
                     /translation="MKLANSRSRSQGNGRVRGRYLARRLEILRAAGKRFRVHGFAETG
                     MRDIAEAAALSPANLYNYFQGKHEILFFCQDNSLDRLIAALDKARRLRTSAAERLRLV
                     IVSHLRCLLDEVEGSAAHLLTSALPPRMQRTLVAKRDRYEEGVRQLIAAGARSGEFVH
                     CDPALAARAVLGALNWSVRWFSPEGPLTAAEIAEGFADYLIRGLLARPAAFRRASSAR
                     ETQRAVPGYLPQNVNRRASAG"
     gene            complement(9434..10690)
                     /locus_tag="VGR03_06395"
     CDS             complement(9434..10690)
                     /locus_tag="VGR03_06395"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1993939.1"
                     /translation="MGAGTIIVGTGQAGFQTAASLRENGYQEPITLIGEEPHIPYQRP
                     PLSKGFPLGTQDIESIELRPKRFYQDHAIHLMPGERVTAIDRAERQVRVASGSRVPYQ
                     KLVLAVGARNRILPLKGADLDGVLYLRSLDEAVVVKERLQHAQEVVVIGGGFIGLELA
                     AVACSLGKSVTVLEVLPRLMSRVVAPIISDFYRHLHTSRGVRVLCSSSVSEISGSNGK
                     VQRVVLSDGTIYPADTVLVGIGVVPNADLARDAGLRIANGIAVNEHLQTDDEKIYAIG
                     DCAEHPCVFAGARIRLESVQNAADQAQCVAAAIAGRPASYRALPWFWTDQFEIKLQMA
                     GISQGHDRIVTRGNPESQKLSVFYFKENRLVAIDSINRPVDHMIGRKLIGAGVPVTPE
                     QAADESVDLRKLGQMAGQPASRPGIH"
     gene            complement(10690..>11029)
                     /locus_tag="VGR03_06400"
     CDS             complement(10690..>11029)
                     /locus_tag="VGR03_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEV1993940.1"
                     /translation="RGSIKMPKVIYVSARGDAREVDVPVGMTVMAAALKNGIDGIVAE
                     CGGVCMCSTCHVYVDQNFFPRLSPANDTEEAVLEISAEERQPTSRLSCQIKVTADLDG
                     LIVRLPERQR"
BASE COUNT         2225 a         3354 c         3203 g         2247 t
ORIGIN      
        1 cggagcgggc gttacggtta gctgtgccgc ttcggctctg gtcggcgtgg tgggtgtcgc
       61 gttcttggcg cgctcctcct cgagctgctc gaggcgcgct tccagatgct gtacccgctt
      121 cagcaattcc gccacggttt ccgcttcatt cgggggagcg ccgccggact gctgtgcttg
      181 tattgcgggt gagaggatca gcactgccag taaggcagcc cctccgatcc tcacaaagaa
      241 atggttcaga gccgccgtcg gcaaccgctt tcgtaatgtt ttcagttcca tcgctcgccc
      301 tcctcaaagc ctgcagatac tatcggcagc gagcgaaatt taagtagggc gccggcgcaa
      361 aaatcacgcc cgcagcgacc cggcactcag tcgccggttt gctcgatccg gtcgcatgcg
      421 tttagctgga gagggaccgc tgaacggtac ggtggaagga aggggaacac cgtggtaaga
      481 ggccgccgct gggcccatca atacaaagtt tgttgtccag tctgcaacgc atggtagagc
      541 cgcaagcttg tctccatctc ggcggcgctc gcttcggcac ggcgcaacga gaactcggct
      601 cagttttctt tctggaccct tacccatcga aaaacggtct agtccggcag cgtcaaagtg
      661 ccttgcattt tgttgccctt gacagcgagt cgccagacgc cttttgagta tgagcccacc
      721 agcgtgtgct gatcctcttc caggcaggca cgctaccagt cccgctctgc gggatacgcc
      781 ccgacgcgct gaacaattca gtttctactg tactccacga gccgccgtcg agaacccaca
      841 tgcgcctcta cggtatgcca aagctttcgc cgggcaacat ccacatcagc acgccaagtg
      901 acgccagcac gcttacaaag taagcggtga tcgcgcggct gggctagtcg gccaatgctg
      961 gagaactcga gccaatacga gccaggctgg aggaagcggc aaaaacgtat aacgagtctc
     1021 gccacgccac acggctcaag agaatactga tgacccttcg ctccggcagt gcgattgacg
     1081 gccggataaa gtggacaacg acctccggga tcgaaggtgg tttcttgcgc gttgttccgc
     1141 aaggcaaacg gagatgggaa ggcaaagtac ctgccgacga gagggatcaa gaataccgag
     1201 atgcacttgc ttttctcgtt cgcggtcaag agcccaggcg tccagcattc ttgaaagggc
     1261 atcagggccc gatggcgatt cgtcaaagcg gaaagcgcca gaccgcccca ccgtagccca
     1321 tcgatgggcc tttcgtgaga gcgtcacgag gccggctgaa gctcgtaccg cagctcttgt
     1381 aagcggttcg attcgatggt cggggcgcgc ggattcgaac cgcggacctc ctgcgcccaa
     1441 ggcaggcgcg ctaccaggct gcgctacgcc ccgacaaact gggtcttgat tgtagcgtac
     1501 tttgtaagtc tgcctctact ccgctcagtc cgggcagtgc cacaaccgtg ccagaatcca
     1561 atcgatttcg ttgaattggg acagatgttt tgcaaaatcg ccttctctaa gttattgaaa
     1621 acacgttccg ccactaagtg gatcgaattg ttgaaaacga atccgctgcc agaaaagcag
     1681 gcggactctt tttgttaacg tttccatcgg ttctaataat tccatatgct gcgccacagt
     1741 gcttgctttg gacctgattc ggccccgttt tggacccaag tttttctttg cgactgaaca
     1801 aacgcgcggg catggtaggc tcttcgtcaa tggatttgat tgcgttctac ggagagtcat
     1861 tcagtggccg ggagcgtcta aaccgttaaa atgcgacagc agctcgacgc cggacaattt
     1921 tatggccgca cgctccggag gcgttcattc ggtgatctga ctcttgccga tgtcgaatat
     1981 cgcgctcata gccacctccc gcgtcactcg cacgaacggg cgtacttttg tctgatacgc
     2041 cgcgggactt atgtcgaaag ctattcgcgg aggactcgtg tctgcgcgcc catgatgttg
     2101 gtctttcatc cccccgctga gccacactcc gaggcttttg acgatgaacc agttgtgtcc
     2161 ttcaatgttg aactcggtgc ggaatggctt tgccgtctcc gcgaattagg tggcacgttt
     2221 gaccagccga ttgaattcca cgccgggagg actgtcgcac tcggtcttcg gttgttcaag
     2281 gaattcgcgc gtgggaatat agactcagac ctatcaatcg aaagtatcac gctggagatt
     2341 ctggccgctt acatcagcaa gcgcccgact gcggcttatt ccataaaacc cgattggctc
     2401 aggcgggctt ccgagaccct ggatgcctgc cttgaccaat ctctttcgct tgggagcgtg
     2461 gccaaaagcg tcggcgtgca tcccgtttat ttcgctttga tgtttcgacg tttcaacgga
     2521 tgttcggtgg gtgaatacgt gcgccgccgt cgaatcgaat atgcacgccg gatgctagcg
     2581 aatccggaga ttccattgac ccaagtcgcg ctgggtgccg ggttcgctga tcagagccat
     2641 ttcacgcgca cctataaacg gttcacgggg agaactcctt cgcaggatcg cacctttctc
     2701 aggttcaaga cacctcccga tcccggaagc tagactcagg cgcagaagag caaaatggca
     2761 accgggagga cttccatgcg atgggcgatg ttgaagcgat tcgtgttctt gatcttcatt
     2821 ttacaattgg ccgacattgc ttttgcgcaa caaggccctg cctttgctgc gcctagtagg
     2881 ttagtcgact taggcggatt tcaagtaaat atctattgcg ttggccatgg tcgccctcca
     2941 gttgtttttc ttcatgggtt aggagactat tcgttcgact gggccttagt tcagccagcg
     3001 cttgctgaac acaccgaagc atgtgcgtac gaccgtcctg gacaagcctg gagtgagcca
     3061 ggaccgccac cgcgtggaat cagcacttcg gcgcatgagc ttcacttgct tctaaagcgc
     3121 tcgaatgtca agggaccgta catactcgtc ggtcactcat ggggtgggtt gatcgctcgg
     3181 atgtatgcgc atgagtaccc gaaagaaatt gctgggatgg tgttggttga tagtacgcac
     3241 gaagatgaat atttgtggat cagcggcaag atcctccggc caagatacat gacggatgaa
     3301 gaatgggccg atctcagaag acccctaaag gccccctctc ctgcgccgtc cagcgatacc
     3361 gcaaaacaag ccccggcccc gcagcagcca atcgtcacgc agcttccttc accctatgac
     3421 aaacttccac ccgatgcgca aaggctaagg ttatgggcaa tgtcgctacc ttggagcaag
     3481 aagcgaaatg aaggtggaga cactctggat ttgcggcaag atttcatcgc cacgtacaaa
     3541 gtcagaattc aaagcggaca cccgctcggg agcatcccgt taattgtgtt gagcaagacg
     3601 ccggggattg aagacgacga tgactacacg ccagatcagt tgaaatggaa tcgagatctg
     3661 caggatcaac tggccacgct atctacgaac agccaacatc tagtcgctga ccacagcggg
     3721 catcacatcc aactcgaaga gcccgacatt gtaattgcct caattcttcg cgtcgtcgat
     3781 gcggtgaggg acaagcggcc tcttaggaac gaaccgatct cagcacattg attgggcggg
     3841 caaccgacaa ccgatccgtt attactgctc aaagatttct gaattcgttc gctagggcac
     3901 gcctgtcggt accaatacct caatctaagg tgaaggacac tgcaaatgtt catggaacca
     3961 atccttggcc agcaagcgtg tgtgtgcgcg cccgcagctg ctacaaaggc aggcgcgtcc
     4021 cctttgccag aactgtgcca gaatccaacg cattagtgcc attggggtta gctttgagcg
     4081 aaaagcagat tccccaaatt actgaaaaca cagagaaacc aaagtgggga atggagttgt
     4141 tggagtcggt cagtccgcgc ccaaggcagg cgcgctacca gtcccgctct gcgggatacg
     4201 ccccgacact accgaattca attctagcgt attttgcgag tgtgcttcta ctgtgatggg
     4261 tgatgattca ccgccaacga gtccgcaacg gaaggcagcg gcaaagtccc ggtgatcata
     4321 ggagaggatg ccgtgtcagg cgctcccgct cggtgtgcac gtcatccccg cgcaattccc
     4381 gcagggcgca ggtcacaaat tcggcagtaa ctttcttgcg ccagctcatg tcgaaatccg
     4441 tgttgtcgag gggtttggct atccgggcgg cgagagcggc ggcctccgca atggactcct
     4501 gattcaggga acggcccaac agagatttgg ccgcctccgc agcaaccagc ggtaggcatg
     4561 ctgccgaacc cacgacgatg cgggcctcct cgaccacacc ctcaaccgag aggcgcgcgg
     4621 cggctgccac ggacaatacc gggaagtcga acgagccgcg gcgccgcaat ttccagtacg
     4681 tgcttttcca gccgtgcaga gaatccagca gcacttccgc cagaatctca tccggtcttc
     4741 gccggatgta gtggatgccg tcgttgttgt acaactctga aagcgggact tcgcgctctt
     4801 cggaccgcga gaccaggcgc acgcgggctc cgagcgcgat cagcgccggc gccgtgtcgg
     4861 tggaggaaac cgccatgcat tttggacttc ccggcgcgac ccagcacgtg gtgccgtctt
     4921 tcttcatgca aaaattgatc gccttccgcc actcatagct ctgatcgtag tagttgcagc
     4981 gggtgtccag gcagaggttt ccccccagcg tagccatgtt gcggatctgc ggagtggcca
     5041 cctgagcagc cgcttgccat aaagccgtcc agccatttcg aaagcgggga tctcccgcga
     5101 tctcggcgag cgtgaggcac gcgccgagcc gcgatgccaa ttcgcccaat tgaacctggc
     5161 gcaagccttc cacataacga agactcatca gcatccgcgg gacctgctgc cgccgcttca
     5221 tgttgggaag cagatccgtg ccgcccgcga gcggcatggc gcttgccccc tcgccgtgca
     5281 gaatccgcgc ggcttcctcc agcgtgcgcg gggtgcggaa ctcgaagaga ggcaggcgca
     5341 tcatgagctg agtacctcct ctttcttcgg gcggggtttc gtgcgccggg gaacatcgtt
     5401 caccgcattg ccatcccctc cctcccacgg cggaggaacg cggagggatt ccggccaatc
     5461 gacatgcgga aagtggctcg gtccaaaacg cggtgtgcgg ccggctttct tttccgccag
     5521 cgccttcact attttttccg gcgtgatggg gacttcatcg attctcacgc cgatagcgtc
     5581 gtagacggcg ttggccacgg ccggcatgat gggcagaagc ggaccctgcc cgacttcctt
     5641 ggcgccaaag gggccgcggg gatccggctc ctcgatcagc acggtgacca catcgggcat
     5701 gtcgagcgac gtcgggctct tgtactccag gatcgacgga aacttgtgca ccagcgcgtg
     5761 agaaagcttc ttcggaagac ggcgaaactc ctgctcctcc ataagcgcct cgcccagccc
     5821 catgtaaacg ctgccctcga cctgaccgcg cgcgagcggc gggttcagcg cccgcccgat
     5881 gtcatgcgca atccaaatcc tggggacggt gacccagccg gtcgtcgggt tcacttcgac
     5941 ctcaaccacg gcagccgagt aggaataggt gggagacggt cccacgccgc cgcctttata
     6001 cagcgcggga gatttcggcg gagtgtagga gccaaccgtg ccgatggtcc cgaagcgagc
     6061 ctccgccagg catacagctt cctgaaaggc gaccccgctc tgcggggccg cggaatcaaa
     6121 cacgcgattg tccgcgaagc gcagccgttc tttaggtact tcgagacgtt gcgctaccgc
     6181 ctccgccagc atggccttgg cccgctcggc agcttgtatc gccgcgttgc cggccatcag
     6241 cgtgacgcgg ctggaatacg atcccaggtc aaccggcccc aggtcggtgt ctccggtgta
     6301 cacgcggatg tcgaacgtgg tgacgcccag aacttccgcc acgatggcgg ccaacatatc
     6361 gtccgagcct tgcccgatct ccgtggcgcc acaaaaaatc gtgacgccgc cgccgcggtc
     6421 gagcttgagc tgaactccgg agtgcggcat tttgttccag tagatgggaa gtcccgcgcc
     6481 ggtcagatac gcggagcagg ccaatcccac gccccgcccc tcgggcagtt tgcggaattt
     6541 ctcattccag ccggagacct ggaccacgcg gcggatgcat tcggagagat tgacggttcc
     6601 cagccggaga aaattcgccg tcacggtgtt gggggattcg acgatgcgca gccgcagctc
     6661 ggcggggtcg atcttgagct tctcggcgat cttatccatc tgcacttcct gaccgaagcg
     6721 cggctgcacg gtgccatggc cgcgcttggg cccgcagggc ggcttgttgg tgaaggtgcg
     6781 gcagccttga aagcgatagc gcggaacgtg gtaggtggtc gtctgcagcg cgccggtgta
     6841 aaacgtgctg gccacgccgt aggagccata ggcaccgcca tcaatgagcg tctggagatg
     6901 catcccggtg atcgtgccgt ccttcttgac gccggtcttg aacttcatca ggacgggatg
     6961 gcggccccgg tgacaataaa atacctcctc gcgcgtcaga cagatcttga ccggccggtc
     7021 cagcaccagc gccgccttgg acacgacaat ctcgtgctgg aaaggatcgc tcttccctcc
     7081 aaaccctccg ccattgggcg tggcaatgac gcgaatatgc gcggctggca tttccagcac
     7141 cttggcgagc gcgcggtgaa cgtaatgagg cgtctgcgtg ctggaccaga tggtcagttt
     7201 tccttcggga tcctttatgg ccacggcagc atgctgctcg atggggagat gagtgctgcc
     7261 ctggtagaaa aaagtgtctt cgaagatttc atccgcctcg gcaaagcctt tttccacgtc
     7321 gccgaagtcc agcgccacgc gtttatgaat atttccttct tcgccgtagt catgaatccg
     7381 cggctccggt gtcgccagag cctcttcggg atcggagatt gtcgtcaaga gttcatactc
     7441 gacatcgatc aggtccaaag cctcgaaggc cgtcagctcc tcccgcgcaa tgaccgccgc
     7501 gaccggatcg ccaacgtggc gcacgtgatc gacgcacagg gcctgctcgt cctgactgat
     7561 gggaagaatc ccgtactcga tggagacgtc tttcccggta aggaccagat acaccccggg
     7621 atgagcgagt gcgcgcgagg catcgatttt tcggatgcgc gcgtgcggat ggggagagcg
     7681 cagcagcttg cagtgggcca tgcgcggcag gaagatgtcg tcggcgaagc gcgtttgtcc
     7741 cgtgaccttt gcgcgggcgt cgacccttcg ccgggggaca ccgatcacgc gcagttcgtc
     7801 cccgttcggc tttgaatctc gagccggccg gtctggcata tccttctgat tcggcatgtg
     7861 ccctcttacg cgcctgggag tgcgcccgca ctcctgcaat ctatgctgct gattcctttt
     7921 gctccgcaat cttgatcatg gcggcttcga cggcttcaaa aatctggaga tagcccgtgc
     7981 agcggcacaa ggtgccggaa agcgcctcac ggatctgatc gcggctggga tgcggattgt
     8041 gatcgagcag cgctttcgcc gtcactaaaa tgccgggcgt gcaatacccg cattgcgcgg
     8101 cgccgagatc ggcaaaggca tcttgcaaag gatgaaggtt gccatctgcc cccaggcctt
     8161 ccacggtcag cacgtctctt ctgtcgcact cgagcgccag caccagacag gaaagcaccg
     8221 gcttgccgtc cagaagaacg gcgcaagcgc cgcactctcc cagctcacag ccatgcttcg
     8281 tgccggtgtg gcccagatcc tcgcgaagga cctcgagcag ggtcttgtac ggcgcgaaag
     8341 agacgtcgac ctcttcgccg ttcaacgtca tccggatgtg agccgttttc tgcgatgcgg
     8401 caggcagcgt gatcaagttc attcattcct ccggaaaatc atccggcaga cgcgcgtcta
     8461 ttcacgtttt gtggaaggta ccctggcacg gcgcgttgtg tctccctggc tgacgaggcg
     8521 cgccggaaag cagccggcct ggccagcaag ccgcggatca gatagtccgc aaatccctcc
     8581 gcgatctccg ccgccgtcag cggtccttcg ggactgaacc agcgcacgct ccaattcaga
     8641 gcgccgagca cggcgcgcgc ggccagcgcc ggatcgcagt gcacgaactc tcccgatcgc
     8701 gcgccggcag cgatcagttg acgcacgccc tcttcgtaac gatcgcgttt cgccaccaga
     8761 gtccgctgca tgcgcggcgg caacgcactg gtcaagagat gcgccgcgga tccttcgact
     8821 tcgtcaagga ggcatcgcaa gtgcgacacg atcaccaggc gcaatctctc cgccgcgctg
     8881 gttcgcaagc ggcgagcctt gtcgagcgcc gcgatcaagc gatctaggga attgtcctgg
     8941 cagaagaaga ggatttcgtg cttgccctga aaatagttat agaggtttgc cggagagagc
     9001 gccgcagcct cggcaatgtc acgcatgccg gtttctgcaa acccgtgcac gcggaatctt
     9061 ttccccgcgg cgcgcagaat ctccaggcgc cgcgcaagat agcgtccccg aacacggcca
     9121 ttgccctgac tcctggatcg gctgtttgcc aatttcattc tctccacggc tggaacctgt
     9181 agccgcctgc accgggtgcc gcttgtttac agttaccatg ctcctcagac ccctgtcaac
     9241 agattctgaa cagcagttca gtaaatgaac aactgttcag attgctgcag cacgattccg
     9301 tagcgccggc gtcattttga accagccacg aagtgcaaaa ccgccgccga gacgccggcg
     9361 ttacgaaggg cagtacgacg acctgggaga gtaaaggaca gtcgtggcct gattccgtgg
     9421 tgagagatgc gtgctagtgg atgcccgggc ggctggcagg ttgccccgcc atttgcccca
     9481 gcttcctcaa atcaacgctt tcatcggcag cctgctcggg ggtcacgggc actccggccc
     9541 cgatcagctt acgtccgatc atgtggtcaa ccgggcgatt gatcgagtcg atggccacca
     9601 gccggttctc tttgaagtag aaaacagaaa gcttctggct ttccggatta ccccgggtga
     9661 cgatccgatc gtgcccctgg gatattccgg ccatctgcag tttgatctcg aactgatcgg
     9721 tccaaaacca cggcaaggcc cggtaactgg ccggccgccc ggcaatcgcg gcggcaacac
     9781 actgcgcctg atcggccgcg ttctgtacgg attcgaggcg gattcgcgcg ccggcgaaaa
     9841 cgcagggatg ctcggcgcag tcgccgatgg catagatttt ttcgtcatcg gtctgcaaat
     9901 gttcgttcac ggcgatgcca ttggcgatgc gaagtccggc gtcgcgggcc agatcggcat
     9961 tgggcaccac gccgatgccc accaacaccg tatcggccgg gtatatcgtg ccgtcgctca
    10021 acacgactct ctgcaccttg ccgttcgagc cgctaatctc cgacacggac gaactgcaca
    10081 aaactcttac gcctcggctg gtgtgcaggt ggcgataaaa atccgagatg atcggagcga
    10141 ccaccctcga catcagccgc ggaagcacct ccagaaccgt caccgacttc ccgagcgagc
    10201 aagccaccgc ggcaagctcc agaccgatga aacctccgcc gatcacgaca acttcctgtg
    10261 cgtgctgaag gcgctctttc accacaacag cttcatccag cgaccgcagg tagaggacgc
    10321 catcgaggtc cgcgcctttg aggggaagaa tgcggttgcg tgctccaacc gccaggacaa
    10381 gcttctggta gggaactcgg ctgcccgacg ccacccggac ctgccgttcg gcccggtcga
    10441 tcgccgtcac tctctccccg ggcatgagat ggatggcatg gtcttgataa aacctttttg
    10501 gccgtaactc gatgctctca atatcctggg tgcccagcgg gaaacccttc gaaagcggcg
    10561 gccgctgata ggggatgtgc ggctcttcgc cgatcagcgt tatcggttcc tgatagccgt
    10621 tttcccgcag ggacgccgcg gtctggaacc cggcctgccc ggtgccgacg atgatggtgc
    10681 ctgctcccat caccgctgtc tctccggcaa gcggacgatc aggccatcga gatcagccgt
    10741 gaccttgatc tgacaactga ggcggctggt gggctggcgt tcctcggcgg aaatttcgag
    10801 cacggcctct tcggtgtcat tggcggggga caggcgcggg aagaaattct gatcgacgta
    10861 gacgtggcac gtcgagcaca tgcacacgcc gccgcactcc gctacgatgc cgtcaattcc
    10921 attcttcaat gccgccgcca tcaccgtcat gcccacaggc acatcaactt cccgggcgtc
    10981 gcccctagcc gaaacgtaga tgacctttgg catctttata gaacccctt
//
LOCUS       DASYVO010000190         6676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_90140, whole genome shotgun sequence.
ACCESSION   DASYVO010000190 DASYVO010000000
VERSION     DASYVO010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90140"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VGR03_06405"
     CDS             <1..217
                     /locus_tag="VGR03_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993941.1"
                     /translation="GLERAGLVRRHATEDGRRVRLEASAKGTKILQEGRKRRVESLAK
                     ALASLSAEEQRQLSALTDLLNQVIQKL"
     gene            complement(1308..2600)
                     /locus_tag="VGR03_06410"
     CDS             complement(1308..2600)
                     /locus_tag="VGR03_06410"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEV1993942.1"
                     /translation="MSTTTHPFTPEEVMAFVDGELSADRTQSLSAHLDQCAECHEAAA
                     ALRTTLHALSQWRVDPLDSLSQQGLLEAASSNCASDIPSQVQGWLRRLQLLFSRPLPW
                     AFACLLFAFLIGAQLLHQARPSRDSIFAKYKVSPTVPPQPAGPSVANQDYSRSTGGGS
                     GGGDYYSFRSNEVSGGTPVDRAALSQQTGHVGGRSAQPAPMIARTVALSIVVNDFDTS
                     RTSLDAILARHNGYAAGLNVATPQGAARALQASLRIPAPQLVAAVAELKALGRVESET
                     QNGEEVTQQHADLLTRLKNSRETELRLQDVLRNRTGKVKDVLEVEEEIARVRGEIEQM
                     EAEQQTLEHRVNFATIDLKLAEEYKAQLTTPAPSVAIQLRNAIVDGFRSAFESLLALV
                     LFLAESGPTLLLWLMILSFPAWLLCRRYRRSLAMGTSL"
     gene            complement(2597..3286)
                     /locus_tag="VGR03_06415"
     CDS             complement(2597..3286)
                     /locus_tag="VGR03_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1993943.1"
                     /translation="MHEALPALVLDSEAIPRTVMNETPTNQANAAGNAGAASATSDEQ
                     LMLAFSKGSSYAFSELFSRYKQPIYGFFRRRVAESTLAEELTQETFVALLRAASRYEP
                     RALFRTYLYAIGFKILRAHRRKAAFRATFFGQRTSVPDPAKQDATESGLWVRRAVEKL
                     EPMDREILLLREFEQLSYAEIADLLQLPLNTVRSRLFRARTALRNLLEPPVAPSTKDA
                     TQNILQKGERA"
     gene            complement(3590..4180)
                     /locus_tag="VGR03_06420"
     CDS             complement(3590..4180)
                     /locus_tag="VGR03_06420"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEV1993944.1"
                     /translation="MGGDARGANTPTPLGLPYDSYKTGPGDSANLFREPRIAYSAELS
                     VATREFAHSRSSLEEILERHHGYVAKLRMVGQPAGSVLSATLRVPSSEYRSALTELKA
                     VGLVEHEEEAADEITQQHSELEARLVNAQNEEQRLRQLLQNRVDKSSDPATLERQVSL
                     LRGEIERIETERSAFGSRVSFANVFFSLREERTSPA"
     gene            complement(4533..5876)
                     /gene="glmM"
                     /locus_tag="VGR03_06425"
     CDS             complement(4533..5876)
                     /gene="glmM"
                     /locus_tag="VGR03_06425"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV1993945.1"
                     /translation="MAKEFFGTDGIRGVPGTPPLDDASLYATGRSLGAYLKREHGAAH
                     VLIGMDTRESGPHIASLLAAGLAQVGATVAFAGVITTPGVACLVRQNDFQAGVVISAS
                     HNPFQDNGVKLFSHAGMKFPDAVEEELEAGILKHRGEAAPKNPPSLAADESLDAEYLA
                     FLRKRFIPGAKLSGFRIVLDCANGAAFRLGPELFRSLGADVIAMGVSPDGRNINAGCG
                     SLHLEGLQKRVVAEKARLGVAFDGDADRALFVCETGKIVNGDGVLLAAARFLKSQKKL
                     KGDRVVATSMSNLGLERVLARENIALARAAVGDRYVLEEMLRSGNVLGGEQSGHIIFL
                     EDSPAGDGLLTAAKIASLVSMHGKIESLVEGLKDYPQLIVNVKVRSKPPLETLPEVSR
                     ALAEAQSALGDNGRVVLRYSGTEQLARVMVEAEHEADVQRFSQSLANALRSSIGA"
     gene            6128..>6676
                     /locus_tag="VGR03_06430"
     CDS             6128..>6676
                     /locus_tag="VGR03_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1993946.1"
                     /translation="MSATQTPPSIVRFTSSNFSMRVLAAAIILLFFYYAAGVVITLLL
                     SILLAYFLDPAVELLERLGLPRTLGAMIMVLILIAVLAAVGYGLWTRTADFAENWPKY
                     SSVLKQAAGAVEGKIRGIEGQVSQIAPEQGAPSATTVRAEPSVVRTLIFRGIGSLYAL
                     FLEITFMPFLVFFMLAEKREVWH"
BASE COUNT         1424 a         1810 c         2136 g         1306 t
ORIGIN      
        1 gggactggag cgcgctggtt tggtgcggcg tcacgccacg gaagatgggc ggcgggtgcg
       61 tctcgaagcg agcgccaagg gcacgaagat actgcaggaa ggacgcaagc gccgggtgga
      121 atcgctggca aaagcgctcg cgtcactttc tgcggaagaa cagcgacagt tgagcgcgct
      181 aacggatcta ctcaaccagg tcattcagaa gctctagcgt ttttttgacg gataggtccg
      241 cggggattcc tcggcaagcc gggctcgtgc gggccaagcg aatcagacaa tggacgttct
      301 acaaatgcga cgaaacgagc gcaaaaaaag tgaagcagat gatcgtagaa cgcgtctaga
      361 ccgaaagtac ttccgtacca aaaaaagtga ccgtggcggg agccggaagt actggaaaga
      421 atgaacccag cgagctatcg acggagaatc cggtgcagag caaactaggc aagtagtcgg
      481 gagttgtttg ggctttctgg agtaatacaa cggttgtaac ctgtctgctc ctgtgtgtcg
      541 tcctgctgct cgcgagcgca gcagaacggc cggaaaaacc taactgactt cggattactg
      601 taccctccat cccacgcccg agcggccggt ccccccatcg gccgctcatt ttttttgggc
      661 cgccagaaac aacttttcta tgaatgtgca gaatctcacg tccagccatc cgaacggttt
      721 cgttgaatat agaggattcc cggtcgaggg gtcaggcgat actcccggga agtttaggca
      781 gaccactgtt gcaggacggg cgtgcctagt gggcagttcg ctggcggact ccggaaatga
      841 aaaagggggc tggtgcaagc ccccttttcg taattgacgc cccaaaaccg gtaaccgagt
      901 cgaggttcgg ttccggccac cccgggaagc caatcttgta gtgaccaatc agagagcaaa
      961 ccgcatgcca gctatgattg ttccgcgtgg aactctcgac ttgattgaaa ccacggaggt
     1021 tagccgcagg cgtggtctcg aaagcactat gttacatacc gcaagcctac aaaatcccac
     1081 agaatggcgg cgggactctc cattctttgg gagcgtttct ttgtgctctt tcgcgatgga
     1141 gcctcggcaa gctcgatctc cagcaccgcg acccgcttgc tccgggcgtt gaaactgaga
     1201 atacagtcat ggcaggtctt ggatgaggct tactgctcaa atctttcgtg aacgttaacg
     1261 gaaatcctct cctgctcgat cggaggctcc tggcgaagga ttcaacttca aagagaagta
     1321 cccatcgcga gggaacgccg ataacgccgg cagagcagcc aggctggaaa cgacaggatc
     1381 atcagccaga gcaacagagt gggaccggat tcggcgagaa acaggacgag cgcaaggaga
     1441 ctctcaaagg cgctgcggaa gccatcgacg attgcgttgc gaagctgtat cgcaacggag
     1501 ggggcggggg tggtgagctg cgccttgtac tcttcggcaa gcttcaaatc gatggtggcg
     1561 aagttgacgc ggtgctccag tgtttgctgc tcggcttcca tctgctcgat ttcgccgcga
     1621 acgcgtgcga tttcctcttc gacttcgagg acgtccttca ctttgccggt gcggttgcgg
     1681 agaacgtcct gaaggcgcag ctcggtttcg cgtgagtttt tcaggcgtgt gagaagatcg
     1741 gcatgctgct gtgtgacctc ttcgccgttt tgagtttcgc tctcaacacg cccgagggct
     1801 ttgagctccg caaccgcggc gacgagttga ggcgcgggga ttcgcaacga agcctgaagc
     1861 gcgcgcgcag cgccttgcgg cgttgcgacg ttcagcccag cggcgtagcc attgtggcgg
     1921 gcaagaatgg cgtcgagcga agtgcgcgaa gtgtcgaaat cattcacgac gatggagagc
     1981 gcgactgtgc gggcgatcat gggagcgggc tgtgccgaac gaccgccgac atgaccggtc
     2041 tgctggctta aggcagcgcg gtcgacagga gtacctccac tgacttcatt acttctgaaa
     2101 ctgtagtagt cgcccccacc acttcccccg ccggtggagc gcgaatagtc ctggttagcc
     2161 acggaaggtc ccgctggctg tggagggacg gtcggagaca ccttgtattt cgcgaagatg
     2221 ctatcgcggg atgggcgtgc ctggtgtaag agttgcgccc caataagaaa tgcaaataag
     2281 agacaggcga acgcccaagg cagtgggcgg gagaagagca gctggaggcg gcggagccag
     2341 ccttggacct gcgagggaat gtccgaagcg cagttggaag atgcagcttc gagaagaccc
     2401 tgttgggaca gagagtcaag tggatcgacc cgccactgag agagtgcgtg caaagtagtt
     2461 cggagagcgg cggcggcttc atggcactcg gcgcattggt cgaggtgggc ggaaagtgat
     2521 tgcgtgcggt cggcggagag ttcgccatcg acaaaagcca tgacttcttc gggtgtgaag
     2581 ggatgcgttg tggtgctcat gcgcgttccc ctttctggag gatgttttgc gtggcgtcct
     2641 ttgtcgaggg tgcgacaggc ggctcgagca aattgcgcag cgcggtgcgg gcgcggaaga
     2701 gccgcgagcg cacggtgttc agcgggagct gcaagagatc ggcgatttcg gcgtaactca
     2761 actgctcgaa ttcgcgcagg aggagaatct cgcggtccat gggttcaagt ttttcgacgg
     2821 cgcggcggac ccacaggccg gactcggtgg cgtcctgctt ggcgggatcg ggaacggagg
     2881 ttctttggcc gaagaacgtg gcgcgaaagg cggctttgcg acggtgggcg cgtagaatct
     2941 tgaaacctat cgcatagaga tacgtgcgga aaagggcgcg gggttcgtag cgggaggccg
     3001 cgcgcagcag ggcgacgaaa gtctcctgcg ttaactcctc cgccagagtg gactcggcga
     3061 cgcgacgccg aaagaacccg tagatcggct gcttgtagcg cgaaaagagc tccgagaaag
     3121 cgtaggagga gccctttgaa aacgccaaca tgagctgctc gtcgctcgtt gctgacgcag
     3181 ccccggcatt gcccgcggca ttcgcttgat ttgtgggcgt ctcgttcatg acggttcggg
     3241 gaatcgcctc cgagtctaga acgagagcgg gcagtgcctc atgcatggta agccgcctgg
     3301 ctcctttact aagtgtaatg tcgcgaggcg gctaatagtt cccggaccag gctcaatttc
     3361 tcaacctgat ccccagccag atttatcatg aattcttggg agcttcggct tcgctaaagg
     3421 ccaactgggc ccgacggcgc caaaagtatc gcgccggaaa atagagcagc accgcccaca
     3481 gagcgagcag cggacctcgg ccggccaaga acagcaggat ggtcgagatg ctctcgaaag
     3541 cgtcgttcaa accgccgacc gcggcattgc gaagtttcgc tccgatcgtc taggcgggcg
     3601 aggtgcgttc ttcccgcagg gagaagaaaa cgtttgcgaa agagacgcgg cttccgaaag
     3661 cagagcgctc cgtttcgatt cgctcgattt caccgcgaag cagagaaacc tggcgttcga
     3721 gcgtcgcagg atcgctggat ttgtccactc gattctggag caactgccgg agacgctgtt
     3781 cttcgttctg cgcgttcacc aggcgcgcct cgagttcgct gtgctgctga gtaatttcat
     3841 cggcggcctc ttcctcgtgt tcgacgaggc cgacggcctt taactccgtg agagcggatc
     3901 ggtattccga cgaagggacg cgcagcgtcg cggaaaggac gctccctgct ggctggccca
     3961 ccatgcgcag ctttgccaca tagccgtggt ggcgctcaag aatctcttcc aagctggagc
     4021 gcgaatgggc gaactccctc gtcgcgacag aaagttccgc ggaataggcg atccgcggtt
     4081 cccggaacaa attcgctgag tcgccgggtc ctgttttata agagtcgtat ggcaagccta
     4141 gcggcgtcgg cgtattcgcc cctctagcgt caccgcccat cgatcgaata cctccttgcg
     4201 gcaggaattc gctgttcggg ctcacgtgat ccggcgcgaa aggaacggcg ggcggatgtc
     4261 cgggccgtac tctctgattc ggcgcgaaca cgaaaaagag agaagtgccg gctaaaagac
     4321 atcctgctaa aaacaagcgg cgccgggccg gaaaaattgc acggaaacga acgacaatgg
     4381 atcgcagacg gtcttccatg gaaatgcctc ctgatacgtc accgttagaa cggggagcac
     4441 gcgaattctt gcagcgcttc cacatcgaaa aatagacgag gcgggccggg aacactttat
     4501 agatgaaaag aagcggtctt tgcaggcgaa atttacgcgc cgatagagga gcgcagggcg
     4561 ttggcgaggg actggctgaa gcgctggaca tcggcttcgt gctcggcttc gaccatgacg
     4621 cgagcgagtt gctcggtgcc ggagtagcga aggacgacgc ggccattgtc gccaagtgcg
     4681 gattgagctt cggctaaggc gcgggagact tccggaagcg tctcgagcgg cggtttcgag
     4741 cgtaccttca cgttgacgat cagttgcgga taatccttga ggccttcgac cagcgattca
     4801 attttgccat gcatggagac gagcgaggca atttttgcag ccgtgagcag gccgtcaccg
     4861 gcagggctgt cttcgagaaa aataatgtgg ccagactgtt cgcctcccag cacgttgccg
     4921 ctgcgaagca tttcttcgag gacgtagcga tcgccgacgg cggcgcgggc gagcgctata
     4981 ttttcgcgcg cgagaacgcg ctcgagaccc agattggaca tggaggtagc gacgacgcgg
     5041 tcacctttga gctttttctg agatttgagg aagcgcgcgg cggcaagaag tacaccgtcg
     5101 ccattcacaa ttttccctgt ttcacaaacg aagagcgcgc ggtcggcgtc accgtcaaag
     5161 gccacaccga gacgcgcttt ttcagcgacg acgcgtttct gcaagccctc caggtgcagc
     5221 gagccgcagc cggcgttgat gttgcggccg tcgggtgaaa cgcccatggc gataacgtca
     5281 gcgccgagcg agcggaacag ttccggtcca agcctgaagg ctgcgccatt ggcgcaatcg
     5341 agaacaatcc gaaaaccgct gagcttcgcg ccgggaatga accgcttccg gagaaaagcg
     5401 aggtattccg cgtcaaggga ttcatcggcg gcgagtgatg gcgggttctt cggcgcggct
     5461 tcgccccggt gtttcaagat gcctgcttcc agctcttctt cgacagcgtc ggggaatttc
     5521 atcccagcgt gcgaaaacag cttcacaccg ttgtcttgaa agggattgtg tgaggcggag
     5581 atgaccacgc cggcttgaaa atcattctgg cgaacaagac aagccacgcc gggagtggta
     5641 atcacaccgg caaaggcgac ggtggcgcct acctgcgcga gacccgccgc gagcaaggag
     5701 gcgatgtgcg gaccggattc gcgagtgtcc atgccgatga ggacatgagc cgcgccatgt
     5761 tcgcgcttga gatacgcgcc gagagagcgt ccggtggcat agagactggc gtcgtccaga
     5821 ggcggcgtgc ccgggacgcc gcgaatcccg tcggttccaa aaaactcttt ggccatggga
     5881 agagacctgc tctccagagt gtggtccggg cccggtcgcg gccaacttcc gtgtggatga
     5941 cctcagaaaa gcttaacaca cgtaaaaacg gtgactttac ggtccggcgc gcgcatgtct
     6001 tttgcataaa cgggacaaat ggaaagcccg gtcaaacaaa tcgttggatt tgatgtatcg
     6061 aaacagccgc gcacggcgcc gtaaacagtt tgcgagagtg tgctaacgtg gagcaccgga
     6121 gccgctcgtg tccgccacgc aaacgccacc atcgatcgtt cgcttcacca gttcaaactt
     6181 cagcatgcga gtgctcgcgg cagccatcat tctgctgttc ttctattacg ccgcaggcgt
     6241 ggtgatcacg ctgttgctct cgattctgct ggcctacttt ctggatccgg ccgtggaatt
     6301 actggaaaga ctggggctgc cccgaacgct gggcgcgatg atcatggtgc tgatcctgat
     6361 cgccgtactg gctgcggtcg gctacgggct atggacgcgg accgcggatt tcgccgagaa
     6421 ctggccgaaa tatagcagcg ttctgaagca ggctgccggc gccgtggaag gcaaaatcag
     6481 gggaatcgaa ggccaggttt cgcagattgc gccggagcaa ggcgcgccgt ccgcgacgac
     6541 ggtgcgggcg gaacccagcg tcgtgcgcac gctgatcttc cgcggcattg gctctctgta
     6601 cgcgctgttt ctggaaatta cgttcatgcc ctttctggtt ttttttatgc tcgcggagaa
     6661 gcgcgaggtt tggcac
//
LOCUS       DASYVO010000191         9789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_117365, whole genome shotgun sequence.
ACCESSION   DASYVO010000191 DASYVO010000000
VERSION     DASYVO010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9789
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117365"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VGR03_06435"
     CDS             <1..1053
                     /locus_tag="VGR03_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1993947.1"
                     /translation="RDVAKSSGFSPATVSIVLNNAPLARYIAPATKKRIEEVAKKLGY
                     RPNAMARFLRSKRSHSVGVMFFDITDPFCTPVLRGIENALYQSSYVQIFADAHNQRNR
                     FERYLELLLEHHVEALIVVANWLFVDIQLLADLSKRNITAATIGWEMPGNAVSSVMVD
                     NEAGGRLALEHLHQLGHRKIAFIRGPKMLIDSAPRWKGIQKFAHSAKLAIDPALVLQL
                     PDSFDPNSGFEGGYRFTEELLQRKKRFTALMAFDDLTALGAIRALTKAGVKVPENCSV
                     VGFDDVPLSSLSAPSLTTVRQPLEAMGNLAVNIVMEGVNARLEKRDWNISRHKMNPEL
                     VIRDSTRAAVLSDSDD"
     gene            complement(1193..2122)
                     /locus_tag="VGR03_06440"
     CDS             complement(1193..2122)
                     /locus_tag="VGR03_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993948.1"
                     /translation="MMKKTNPNRIARMIGALALVSGLFVASAVAAGPGTVTMTVTAVG
                     KKDTTPPIVTKDDVQLFLNKERTQVADWRHGEKLYLAVLIDDSLDSGIASQWSDLKAF
                     LAGQPDTTYISVAYARNGTAMLAQDFTNDHELAAKALRLPLGGGGAFSSPYLALLDLM
                     KRWPSSADRRSILLISSGIDYFRGDFGTRSPDLDSTISRAQKQNINIWTIYAPDTGHR
                     ARGFFLISRAQSNLSQLSDETGAESYYLGTGAPVTLKPYFDELSTHLSNQYLLTFKAS
                     GGAKGRFERARVATELPYVEFLAASQAFLPAVQ"
     gene            2315..3817
                     /gene="glpK"
                     /locus_tag="VGR03_06445"
     CDS             2315..3817
                     /gene="glpK"
                     /locus_tag="VGR03_06445"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV1993949.1"
                     /translation="MKNYIGAIDQGTTSTRFMVFDQSARIAAVVQKEHEQIFPQPGWV
                     EHDPLEILRRTEEVIAGALEQRGLRASDLAAIGITNQRETTVLWERKTGKPVANAIVW
                     QDTRVSEDVARFASEGGQDRFRPQTGLPLSTYFSSLKLRWLLENIPGAREKAAGGELL
                     FGNIDTFLLWNLTGGSRGGVHATDVTNASRTQLLNLKTLDWDAEILAAFDIPRAVLPT
                     VRSSSEVYGEATLGAIAGVPITGILGDQQAALVGQACFRPGEVKNTYGTGCFLLMNTG
                     ERPVPSNFGLLTTVAYKFGGGAAHYALEGGVAIAGALVQWLRDNLGIIEKSPDVEALA
                     RTVNDNGGVYFVPAFSGLYAPYWKENARGVIAGLTRYANKGHIARAVLEATAFQTREV
                     VEAMEKDSRIELASLRVDGGMVANELLMQFQADILNREVVRPVIQETTALGAAYAAGL
                     AVKFFSGLEELRANWAVDRTWKPHLAETEREQQYRQWKKAVTRSFDWIES"
     gene            3833..4564
                     /locus_tag="VGR03_06450"
     CDS             3833..4564
                     /locus_tag="VGR03_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEV1993950.1"
                     /translation="MASPLAGEFLGTMILVLLGDGVVAAVLLKRSKAEGSGWMVITAG
                     WAFAVMAGVFTAIACGSSDAHLNPAVTIGFAVRDGNFAKFAPYLAAQMLGAIVGAALV
                     WLHYFPHWKETPDVGAKLGCFCTAPAIRNTVTNLLSEIIATFVLVFVVGAIFSKSVSA
                     AGPAAGLGPYVVGSLVWGIGLSLGGPTGYAINPARDLGPRIAHAILPIAGKGGSDWGY
                     APIPVIGPLIGGLLAGVLLRVLAVS"
     gene            4655..6883
                     /locus_tag="VGR03_06455"
     CDS             4655..6883
                     /locus_tag="VGR03_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993951.1"
                     /translation="MESTLTTAPPQSALPPFSRQSPWIYRPWIDLLIGCGAWSAPLLL
                     LAFYATNTYARAWAVAFYFLALLCNYPHFMATVYRAYHTRTEFEKYHIFTVHIALLLA
                     LAGVIAHVWYPLLPWIFTLYICWSPWHYTGQNFGLLMMLARRSGLAPSSAERNAIYLS
                     FIASFLLLMLSFHTGSSGDALILSLGLPAKVTLPSRGVLAVFFLGASGWALISMARRS
                     SLRAILAPLTLAVTQFLWFLLPATIELFSGHEVPQTRYSSGVLAVLHSAQYLWIASYY
                     QEREARERGDTNWTFPRYLLTLVAGGIALFIPGPWIASRIFHADFAASFLTFTALVNI
                     HHFILDGALWKLRDSRVAAFLLNSNEKTQGAESQERGAFQSAAHWLTGSAPAARGIRI
                     ATVALLLAWAAMDQLHFYWSSEADSLPALKRAARLNPDDSAVLTRLAHAETLAGKRDA
                     SLAALRRAAAISPGSFPQQEAYARGLIEAGREAEAYAQYEKMLGHWPRSIDALVNYGL
                     LAQRQGQGNEAIDSWQRAIDIDPGQANAQIYLAQALDKRGETQAAARHYRAYLQIVAA
                     HRDEHGSEGTTVIAALIKVADADGAAHQQDEALQGYNAAIQFAEKTNAKTLESLALVH
                     LADLQAKRESIAAAAGAYQHALAIDASLSDPVSAGSDWFNYGQFLRRQHQPERLVFAC
                     FLRAEELVNTTPGAELTTIAQARAESEARLGREAAAVRRSSGATAKEALDLPASSFSR
                     PR"
     gene            7106..7933
                     /locus_tag="VGR03_06460"
     CDS             7106..7933
                     /locus_tag="VGR03_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEV1993952.1"
                     /translation="MPGEALDDALGAAQTLREKKIGTVFTHLGENVKDSSEAQQVTED
                     YLEVLERIREKGLQAEISVKLTQLGLDLSPDICFEHLKAIIERAQKDSIVWVDMEASN
                     YVDATLDLYRRALTAHPNVGICLQAYLHRTKDDLAKLLPLRPSIRLVKGAYNEPPEIA
                     FARKQDVDESYFELGKQMLRAKKENCCGRAAFGTHDVALIRRLANFALAEGFAKRDFE
                     VQMLYGIQRAEQERLASEGCTSIVLVAYGSYWYPWFVRRLAERPANLWFMVRSVFAA"
     gene            7955..8923
                     /gene="rbsK"
                     /locus_tag="VGR03_06465"
     CDS             7955..8923
                     /gene="rbsK"
                     /locus_tag="VGR03_06465"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEV1993953.1"
                     /translation="MMAKQSFIAVVGSANVDLTTFNDVFPQPGETIFGKKFDLGFGGK
                     GANQAVAARLCGANVGMVAKVGSDLFGPATIKNFESQGIGATYVRIAEGVSSGVAPIF
                     VDASGQNRIIVVKGANDSLSPEDVDAAASLLRKADTIIMQFEIPLRTVYHTVKFAKAN
                     GIRCIVNPAPAQPVDYKEIGAADYFIPNESEAETIAGMPVHSVDDARKCAEFLLRQGM
                     QRVVITLGERGSLAAGPDRMELIPAFKVQAIDTTGAGDAFIGSFAVFLSEGLPEEEAL
                     PRANLYAALSTTKVGTQKSFCNRAEFEKEWRNRSSRLQTGFVKVLR"
     gene            8964..>9789
                     /gene="deoC"
                     /locus_tag="VGR03_06470"
     CDS             8964..>9789
                     /gene="deoC"
                     /locus_tag="VGR03_06470"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV1993954.1"
                     /translation="MTLRDSTRIDNSSSLDRQVLRGPISPSDTAIANYAGRDLQPNRG
                     VPLNLDWVNAVRVNTSAVERRAQTIGTRRTVKKEWQAAWLLRAVTCMDLTTLSGDDTD
                     ERVRRLCAKARQPIQQELMQKLGIESLDIKVAAVCVYHTFVETARRAIEGSGIHVAAV
                     STGFPAGLSPFGERVAEIHRSVEAGADEIDVVITRAHVFGGKWQALYDEIVAFKNACG
                     PAHLKVILGTGDLLTLRNVARASVVAMMAGADFIKTSTGKEATNATLPVSLVMTRAI"
BASE COUNT         2000 a         2756 c         3037 g         1996 t
ORIGIN      
        1 agggatgtcg cgaagtccag cggcttctct cccgccacgg tctcgatcgt gctgaacaat
       61 gcgccgctgg cgcgctacat cgcgccggcc acgaagaaac gcattgaaga agtggcgaag
      121 aaacttggct accggccaaa cgcgatggcg cgttttcttc gcagcaaacg gagccatagc
      181 gtcggagtga tgttcttcga cattaccgac ccgttttgca cgccggtctt gcggggcatt
      241 gagaacgcgc tctatcaatc gtcgtacgtc cagattttcg ccgacgccca caaccagaga
      301 aaccggttcg aacgatacct tgaactgctg ctggagcatc acgtcgaggc attgatcgtg
      361 gtagccaact ggctcttcgt ggacattcaa ctgctcgcgg acctcagcaa aagaaacatt
      421 accgcggcga ccatcggctg ggagatgcca gggaacgccg tcagctcggt gatggtggac
      481 aacgaggcag gagggcggct cgcgctcgaa catctccacc agctcgggca ccgcaagatc
      541 gcgttcatcc gcggcccaaa aatgctgatc gacagcgctc cgcggtggaa agggatacag
      601 aaattcgccc actcggccaa attggcgatc gatccggcgc tcgtcctgca actgccggat
      661 tctttcgacc ccaactcggg ctttgaaggc ggctaccggt tcacggaaga attgctgcag
      721 cgaaagaagc ggttcacggc attgatggcc ttcgacgacc tgacggcgct gggagcgatc
      781 cgcgcgttga ccaaagcggg cgtgaaagtg ccggagaatt gctcggtagt gggtttcgat
      841 gacgtgccgc tgtcctcgct gtccgcgccg tctttgacaa cggtgaggca gccgctggaa
      901 gcgatgggaa acctggcggt gaatatcgtg atggaaggcg tcaacgccag gctggaaaaa
      961 cgcgattgga atatttcgcg gcacaaaatg aacccagaac tggtgatccg cgattccacg
     1021 cgggcggcgg tgctcagcga ctctgacgac tgagaattac tttccctcag agaagttcaa
     1081 aatcagttcg gccgggcaga accgcgcggg actatttttc caatcacaaa gaaaaaaggc
     1141 gcgaaccgaa cgatccgcgc ctttcccttt tcaagccacc cgatttcggc gctcattgca
     1201 ccgcaggcag aaaggcttgc gaagccgcca aaaattcaac gtagggcagc tccgtcgcaa
     1261 cccgggcgcg ctcgaaacgc ccttttgctc ctccgctggc tttgaatgtc aggaggtact
     1321 ggttgctgag gtgggtggag agttcatcaa aatagggctt gagggtgacg ggcgcgccgg
     1381 tgccgagata ataggattcg gcacccgttt catccgagag ctggctgaga ttcgattgcg
     1441 ctctggaaat aaggaagaat ccgcgtgccc tatgccccgt gtccggagca tagatcgtcc
     1501 aaatattgat attctgcttc tgcgcgcgac tgatcgttga atcgagatcc gggctcctgg
     1561 tcccaaaatc tccgcgaaaa tagtcaatcc cggaagagat caagagtatg gagcgccggt
     1621 ccgcggaaga aggccaccgc ttcatcaggt caagaagcgc gagatagggg ctcgagaacg
     1681 cgccaccacc gccgagcggc aagcgcaaag ccttggccgc aagctcgtgg tcgttggtga
     1741 aatcctgggc gagcatggcg gtgccattgc gcgcgtaagc tacagagata taggtggtgt
     1801 caggctggcc ggcgagaaat gccttgagat cgctccactg gctggcgatg ccgctgtcca
     1861 gcgaatcgtc gatcaggaca gcgaggtata atttttcgcc gtgcctccaa tcggcaacct
     1921 gggtgcgctc cttgttcaga aaaagctgga cgtcgtcttt ggtaacgatc ggtggggtgg
     1981 tgtctttctt gccgacggca gttaccgtca tggtgactgt tccaggtcct gcggccacgg
     2041 cacttgccac aaaaagcccg gataccaggg cgagtgctcc gatcatccgc gcaattcggt
     2101 ttggattcgt tttcttcatc atcgtagtga ctccttttac caatccccac aaccttagat
     2161 gcaagatcgg gccgaaaggc gtacatggag ggccggtttt caggccaaga gcgggcggag
     2221 cgtttcccct ggacggggat gggcggtctt ttgcatgaag ctaggggtgg cacttgcgta
     2281 gtatgctgcg gcgtcaacaa aaaggaccgg tgcggtgaaa aactacattg gagcaattga
     2341 tcagggcacg acaagcacgc gattcatggt gtttgaccag tccgcgcgga tcgccgccgt
     2401 agtgcaaaaa gagcacgaac agatttttcc acagccgggt tgggtggagc acgatccgct
     2461 ggaaatcctg cgccgcacgg aagaagtgat tgcgggcgcg ctcgagcagc gcgggctgcg
     2521 tgcttcggat ctggccgcga tcggcatcac taatcagcgg gagaccaccg tcctgtggga
     2581 gcgcaagacc ggcaagcctg ttgcgaatgc aattgtttgg caggacacgc gggtttcgga
     2641 agatgtcgcg cgcttcgcca gcgaaggcgg acaagaccgc ttccggccgc agaccggcct
     2701 gccgctgagc acatacttca gcagcctgaa gctacgctgg ctgcttgaaa atattcccgg
     2761 cgcccgagag aaggcggccg ggggcgagtt actgttcggg aatatcgaca cctttctcct
     2821 ttggaacttg acgggcggat cgcgaggcgg tgtacacgct accgacgtga cgaacgccag
     2881 ccgcacgcag ctgctgaatt tgaaaacgct ggactgggat gcggagattc tcgccgcgtt
     2941 tgacattccc cgcgcggtgc tgccaacggt gcgttcgagc agcgaagtct atggcgaagc
     3001 aactctcggt gcgatcgccg gcgttcccat caccggaatc ctgggcgatc agcaggcggc
     3061 gctggtcggc caggcgtgtt ttcgtcccgg cgaggtcaag aacacgtatg gcacgggctg
     3121 ctttctattg atgaatacgg gcgaacggcc ggtgccctcg aatttcgggc tgctcaccac
     3181 ggtggcctac aagttcggcg gcggggccgc gcactacgcc ttggaaggtg gtgtggccat
     3241 tgccggagcc ctagtgcaat ggctccgcga caatctcgga atcatcgaga agagtccgga
     3301 tgtggaggcg ctggcgcgaa cggtgaacga caatggcggc gtttatttcg tacccgcctt
     3361 ttccgggctg tacgcgccgt attggaagga gaatgcacgt ggcgtcatag ctggcttgac
     3421 gcgctacgcg aataaaggac atatcgcccg agcggttctg gaagccactg catttcagac
     3481 gcgggaagtg gttgaagcga tggagaaaga ttctcggatc gagctcgcca gcctccgcgt
     3541 ggatggcggg atggtagcga acgagttgtt gatgcagttt caggcagata tcttgaaccg
     3601 cgaggtggta cgccccgtga ttcaagaaac gacggcgctc ggcgcagcct atgccgccgg
     3661 cctggcggtg aaattctttt ccggcttgga agagttgcgg gccaattggg cggtcgatcg
     3721 cacctggaag ccgcatctcg ccgaaacgga gcgcgagcaa cagtataggc aatggaagaa
     3781 agcggtcaca cgatcgtttg actggattga atcctgacgg aggcacttac ttatggcatc
     3841 accgctggcc ggtgaatttc tcggcacgat gattcttgtt ctgctcggcg atggagttgt
     3901 cgccgcagtg ctgctcaagc gatcgaaagc ggagggaagc ggctggatgg tgatcaccgc
     3961 cgggtgggcg ttcgcggtga tggcgggcgt tttcacggcc attgcgtgcg ggagcagcga
     4021 tgcccatctg aatcccgcag tgaccattgg tttcgcggtg cgtgacggta attttgccaa
     4081 gttcgctccc taccttgcag cacagatgct cggggccatc gtgggtgccg cgctcgtctg
     4141 gctgcattac tttccgcatt ggaaggaaac gccggacgtt ggggccaagc tggggtgctt
     4201 ttgcacggca ccggcgatcc gcaataccgt tacgaatttg ctgagcgaaa tcattgcaac
     4261 ctttgtactt gttttcgtcg ttggcgcgat tttttccaag agcgtttcgg cggctggacc
     4321 cgcggcagga ctcggcccgt atgtggtggg tagcttagtc tggggcatcg gtctgtctct
     4381 gggcgggccg accggatatg cgatcaaccc cgcccgagac ctcgggccgc gcatcgcgca
     4441 cgcgattctt ccgattgcag ggaaaggcgg ctcggattgg ggctacgcgc cgattcctgt
     4501 aattggaccg ctgatcggcg gcttgctggc cggtgtgctt ctccgcgtgc ttgccgtttc
     4561 ctagtccacg ggcgagaaca caagtaaccg attccacatt tcagccgcgg tggctgggcg
     4621 caagaaccgt tccgctgcgc tactcttctg taggatggag tcaactctca caactgcgcc
     4681 gccgcagtcc gcgctgccac cattttcgag acaaagtccc tggatttatc gtccgtggat
     4741 cgacctcctg atcggctgcg gagcgtggtc agccccgctg cttttgctgg ccttctatgc
     4801 gacaaacacg tacgcccggg catgggccgt ggcgttttat tttttggcgt tgctctgcaa
     4861 ttacccgcac ttcatggcca ccgtctaccg cgcgtaccac acgcgcacgg agtttgagaa
     4921 ataccacatt ttcacggtgc atatcgcgct gttgctggcg ctggccgggg tcatcgcgca
     4981 cgtctggtat ccgctgcttc catggatttt cacgctgtac atctgctgga gcccgtggca
     5041 ctacacgggg caaaatttcg gcttgctgat gatgctcgcg cgccgttcgg ggctcgcgcc
     5101 gagttctgcg gaacgaaacg caatctatct gtcgttcatt gcgtcgtttc tcctgctgat
     5161 gctgagcttt catacgggat cctcaggcga cgcactgatt ctctcgctcg ggttgccggc
     5221 gaaggtcacc cttccttctc gaggcgtgct tgcggtgttc ttcttgggcg cgagcggctg
     5281 ggcgctgatt tccatggcgc ggcgcagcag cctgcgggcg atcctcgcgc cgttgacgct
     5341 ggccgtcaca caattccttt ggttcttgct gcctgcgacg atcgaactct tcagcgggca
     5401 cgaagtgccg caaacgcgct acagcagcgg cgttctggcc gtgcttcatt ccgcgcaata
     5461 tctatggatc gccagctatt accaggagcg cgaagcgcgc gaacgaggcg ataccaactg
     5521 gacatttccg cggtacctgt tgaccctggt cgcgggagga atcgcgctat ttattcccgg
     5581 gccgtggatc gcgagccgta tctttcacgc ggatttcgcc gccagcttcc tgacgtttac
     5641 ggcgcttgtg aacatccatc acttcattct cgacggggca ctctggaaac tgcgcgattc
     5701 gcgcgtggca gcattcttgc tcaattcgaa tgagaagacc cagggtgcgg aatcacaaga
     5761 gcgaggcgca tttcaatccg cggcgcactg gctgacgggt tccgcacctg cggcaagagg
     5821 gattcgcatc gcaaccgttg cgctgcttct ggcctgggcg gcaatggatc aactgcattt
     5881 ctattggtcg agcgaggcgg acagcctccc ggcgctcaag cgcgcggcaa ggttgaatcc
     5941 ggatgacagt gccgtgctta cgcgactggc gcatgcggaa acgctggcgg gaaagcgcga
     6001 tgcttcgctt gcggcgctgc ggcgagcggc tgcgatcagc ccgggcagct ttccacagca
     6061 ggaagcatac gcgcgaggct tgatcgaggc ggggcgagaa gcagaagcgt atgcgcaata
     6121 cgaaaagatg cttggccatt ggccacggag tattgacgcg ctggtgaact atgggctctt
     6181 ggcgcaacgc caagggcagg gcaatgaagc catcgatagc tggcagcgag ccatcgacat
     6241 cgaccccggt caagcgaacg cccagatcta tcttgcgcag gcgctcgaca aacgcggcga
     6301 aacacaggcc gcggcgcggc actatcgagc ctatttgcaa attgtggccg ctcatcgaga
     6361 cgagcacggc agcgaaggca ctacggtgat cgcagcgctg atcaaagtcg ctgacgcaga
     6421 cggggctgcc catcagcagg atgaagcgtt gcagggttac aacgccgcga tccaattcgc
     6481 tgaaaaaacg aatgccaaaa cgctggagag cctcgccctg gttcacctgg ccgacttgca
     6541 ggctaaacgc gagagcatcg cagcagcagc gggcgcatat cagcacgcgc tggccattga
     6601 tgcttcgctc tccgacccgg tcagcgctgg gagcgactgg ttcaattacg ggcagttcct
     6661 gcggcggcag caccaaccgg agcgtctcgt tttcgcgtgt tttcttcgcg cagaagagct
     6721 ggtgaacacc acgcccggcg ccgaactcac caccatcgcg caagctcgcg cggaaagtga
     6781 agcgcgcctc ggccgcgagg ctgcggcggt ccggcgatct agtggggcaa ctgctaagga
     6841 agcgctcgat ctgcctgctt cctccttttc gcggccgcgc tagcccgata agtcagcgtt
     6901 tcccgaaaaa tagagaaaca actcgcattg cattgcggtt tagaatggtg agcgggcgta
     6961 agccgcaagc gcttgcattc cagggcgaag gggcgatgca ttcatgagtc tcatgcgatc
     7021 gatactgctc gcagcatctc aaaatcagtg gttacgccat cgcgcggccc attatccgtt
     7081 tgtcaggcgc accgtatccc ggtttatgcc tggcgaggcg ctcgatgacg cgcttggtgc
     7141 ggcacagact ctgcgtgaaa agaagatcgg gacggtcttc acacacctgg gagaaaacgt
     7201 caaggacagt tccgaggcgc agcaggttac ggaagactat ctcgaagttc tggagcgcat
     7261 cagagaaaaa ggtctgcaag cggagatttc cgtcaaactg acacagctcg gtttggatct
     7321 ctcgccggat atttgcttcg aacatttgaa agccataatt gagcgtgcgc aaaaggactc
     7381 catcgtctgg gtagacatgg aggcgagcaa ctatgtggac gcgacgctgg atctctatcg
     7441 gcgcgcgctg acggcgcatc ccaatgttgg gatatgcctg caagcttatc tccatcgaac
     7501 aaaggatgac ctggcgaaac ttcttccgct gcggccctcg attcggttgg tcaaaggggc
     7561 atacaacgag ccaccagaaa ttgcgtttgc ccgaaagcag gatgtcgacg aaagttattt
     7621 cgagcttggg aagcagatgc tgcgggccaa gaaagagaat tgctgcgggc gcgccgcttt
     7681 cggcacgcat gacgtagcac tgataaggcg gctggccaac tttgcattag cggaaggatt
     7741 tgcgaagaga gatttcgagg tgcagatgct ctacggtatc caacgcgccg agcaggagcg
     7801 tctcgccagc gagggctgca cctccatcgt gctcgtcgct tacggcagct attggtatcc
     7861 gtggttcgtg cggcggctgg cggagcgccc ggcgaattta tggttcatgg tgcgcagcgt
     7921 tttcgcggcg taacgtgcct ccaccagaga cgaaatgatg gccaagcaat cctttattgc
     7981 ggttgtgggc agcgccaatg tggacctcac cacgttcaat gatgtgttcc cgcagccagg
     8041 ggaaacgatt ttcggcaaaa agttcgatct cggcttcggc ggtaaaggcg ccaatcaagc
     8101 ggttgcggcg cgtctctgcg gagcaaacgt gggcatggtg gcaaaagtcg ggagcgacct
     8161 tttcggcccg gctacgatta agaactttga atcgcagggg attggcgcga cgtacgtgcg
     8221 catcgccgag ggtgtttcca gcggggttgc tccaatcttt gtggatgcga gcggccagaa
     8281 ccgcatcatc gtagtgaagg gcgcgaacga ctcgctttcg cccgaggatg tagatgccgc
     8341 cgcgtccctg ctccgcaaag cggacaccat tatcatgcaa tttgagattc cgttacgcac
     8401 cgtctaccac accgtgaagt tcgctaaagc caatggcatt cgctgcatcg tgaatcccgc
     8461 tccggctcag ccagttgact acaaggaaat cggcgctgcg gattatttca ttccgaatga
     8521 gagcgaagcc gaaacgatcg cgggaatgcc ggtgcattcg gtcgatgatg caagaaagtg
     8581 cgcggagttt ctgctgcgcc aaggaatgca gcgggtcgtc atcactctcg gcgagcgcgg
     8641 ctctttggcg gcggggccgg accggatgga gcttattccc gcattcaagg tccaggcgat
     8701 agataccacc ggcgcgggcg atgctttcat cggtagcttt gcggtttttc tgagcgaagg
     8761 tttgccggag gaggaagctt taccgcgcgc aaatctctac gctgctctgt ccacaaccaa
     8821 ggtgggcacg caaaaatcct tctgtaaccg cgccgaattc gagaaggagt ggaggaatcg
     8881 cagctcgcgt ctgcaaacgg gctttgtcaa agtgctaaga taggtgtttc ctgattgctc
     8941 cgtcgcatcc ggtgaacaat tccatgactc tgcgcgacag cacgcggatc gataactcct
     9001 ccagcctgga tcgtcaggtt ctacgaggtc cgatttcgcc ttccgatacc gccatcgcca
     9061 attacgcagg gcgcgatctc caacccaacc gcggggtccc gctaaatctg gattgggtga
     9121 acgcggtgcg cgtgaacacc agcgccgtcg agcgccgcgc gcaaaccata ggcactcggc
     9181 gcacagtaaa gaaggaatgg caggcggcct ggctcctgcg cgccgtcacc tgcatggatc
     9241 tgacgacact ctccggcgac gacaccgacg agcgcgttcg ccgattgtgc gccaaggcgc
     9301 gacaacccat tcagcaagaa ctcatgcaga agcttggcat cgagagcctc gatatcaagg
     9361 tcgctgccgt ctgcgtttat cacacctttg tcgaaaccgc gcggcgcgcg atcgagggca
     9421 gcggcattca tgttgccgcg gtctccacgg gattcccagc cggactttct cccttcgggg
     9481 aacgcgtggc ggaaattcat cgctcggtgg aagccggcgc cgacgaaatc gacgtggtca
     9541 tcacgcgcgc gcacgtcttc ggcggcaaat ggcaagcgct ctacgatgag atcgtcgcgt
     9601 tcaaaaacgc ttgcggtcca gcgcatctga aggtgatcct tggcaccggt gatttgctga
     9661 cactgcgcaa cgtggcgcgc gccagcgtgg tggcgatgat ggccggagca gattttatca
     9721 agacgtccac tgggaaggaa gcgaccaacg cgacgcttcc cgtgagtctc gtgatgacgc
     9781 gcgctattc
//
LOCUS       DASYVO010000192         1713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_33728, whole genome shotgun sequence.
ACCESSION   DASYVO010000192 DASYVO010000000
VERSION     DASYVO010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33728"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VGR03_06475"
     CDS             <1..210
                     /locus_tag="VGR03_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEV1993955.1"
                     /translation="LFLGLVFVLAGGSAGTATVIFGIFTGARLLHSIFYLAGKQPWRT
                     IFFIVGGLATIALMLDIIWLMIKGA"
     gene            264..638
                     /locus_tag="VGR03_06480"
     CDS             264..638
                     /locus_tag="VGR03_06480"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV1993956.1"
                     /translation="MLKTIAIVVVVAIAAILGYAMTMPDDFRVQRTTSIKAPPEKIFP
                     LINDLHRWGSWSPWEKMDPEMKRTYSGAAPGKGAAYAWQGNRKVGEGRMEIVDTSPPS
                     KITIKLNFIKPFEGHNTAEFTL"
     gene            850..1209
                     /locus_tag="VGR03_06485"
     CDS             850..1209
                     /locus_tag="VGR03_06485"
                     /inference="COORDINATES: protein motif:HMM:NF018893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEV1993957.1"
                     /translation="MSYVDGFVVPVPRKNLAAYRRMAQKAGKVWKEYGALEYKEWISD
                     DVKVGKLTSFPRSVKLKPGETVVFSYIVYKSRAHRDQVNAKVMKDPRLADMMDPKSMP
                     FDGKRLIYGGFKKLVFV"
     gene            1206..>1713
                     /locus_tag="VGR03_06490"
     CDS             1206..>1713
                     /locus_tag="VGR03_06490"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEV1993958.1"
                     /translation="MSGRAAQSTDEGPQPATINVDDTHRVSGLLQARRGARACYVMAH
                     GAGAGMAHPFMQAMANALAGNGIATLRYQFPYMELGSKRPDTPKLAQATVRAAVAEAS
                     RLVPELALFAGGKSFGGRMTSQAQAASPLSEVRGLMFLGFPLHPAGRPSDERGAHLFD
                     VQIPMLFLQ"
BASE COUNT          362 a          510 c          504 g          337 t
ORIGIN      
        1 cttttcctgg gccttgtctt cgtgctcgcc ggcggcagcg ccggaactgc gacggtcatc
       61 tttggaatct tcaccggcgc gcggctcctg cattccattt tctatctggc ggggaagcag
      121 ccttggcgaa ccatcttctt catcgtgggc gggctcgcca ccatcgctct catgctggat
      181 atcatctggc tgatgatcaa aggcgcttga ggacttccaa ctaccggcac gccatgattt
      241 caacatcgca tcggaggaat cctttgttaa agaccattgc catcgttgtc gttgtcgcaa
      301 ttgccgcgat tctcggctat gcgatgacca tgcctgacga cttccgtgtc caacgtacga
      361 cgagcatcaa agcgccgccg gagaagatct ttcccctcat caacgacctc catcgttggg
      421 gttcctggtc gccatgggag aaaatggatc ccgaaatgaa gagaacctac agcggcgcgg
      481 cacccggcaa gggcgcggcg tatgcgtggc aaggtaacag gaaggtcggc gaggggcgca
      541 tggagatcgt cgatacatcg ccgccatcca agatcacgat caagctgaat ttcatcaagc
      601 cgttcgaagg ccataacact gccgaattta cgctctaacc aaagggggat tccacgaacg
      661 tcacctggac catgtatggc ccgagcatct acgtggcgaa agtcatgggc gtattcgtca
      721 acatggacaa gatgataggc aaggacttcg agaccggact tgccaacctg aaaaccgtcg
      781 ccgaaaccta gaccgcatac acgcgtcacc cgtgcagctg ctgtaaaacc ccgacaacct
      841 ggaggcttta tgagctacgt tgatggcttt gtagtaccgg taccgcggaa gaatctggcg
      901 gcctatcggc gcatggccca gaaggcagga aaagtgtgga aggaatatgg cgccctcgaa
      961 tacaaggagt ggatcagcga cgacgtgaag gtcggcaagc tcacctcgtt tccacggagc
     1021 gtcaagctca agccgggcga aaccgtggtc ttctcgtaca tcgtgtacaa gtcgcgcgcg
     1081 catcgcgatc aggtgaacgc caaggtcatg aaggacccgc ggctggccga catgatggat
     1141 ccgaagtcga tgcctttcga cggcaagcgc ctgatctacg gtgggttcaa gaagctggta
     1201 ttcgtttgag cggtcgggca gcccaatcga cggatgaggg tccgcagcct gcgacgataa
     1261 acgtcgacga cacgcatcgc gtctccggtt tgctgcaagc acggcgcggg gcgcgcgctt
     1321 gctacgtcat ggcgcacggc gcaggcgcgg gtatggcgca tccgttcatg caggccatgg
     1381 cgaacgcact tgccgggaac ggcatcgcca cgctccgcta ccagtttcct tacatggaac
     1441 tgggctcgaa gcggccggat actcccaagc ttgcccaagc cacggtccgc gctgcagttg
     1501 cggaagcgtc tcgtctggta ccggagcttg cccttttcgc gggcgggaaa tcgtttggcg
     1561 gccgcatgac ctcacaagcg caagccgcat cgcccttgtc cgaagtccgc ggcctaatgt
     1621 tcctcggatt cccgctccat cccgccgggc ggccgtcgga cgagcgagga gcgcacctct
     1681 tcgacgtgca aatcccgatg ctgttcctgc aag
//
LOCUS       DASYVO010000193         4783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_143910, whole genome shotgun sequence.
ACCESSION   DASYVO010000193 DASYVO010000000
VERSION     DASYVO010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143910"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="VGR03_06495"
     CDS             complement(<1..1103)
                     /locus_tag="VGR03_06495"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV1993959.1"
                     /translation="MKTRLLVVDDEQSIRRLCMTIGNTLGYVCVEAESAETALARLDS
                     DTPDLLLTDLKLPNMSGVELLKQTKLLLPHTEVAIMTGHGSIESAVDAMKLGAYDYIE
                     KPFRVEKMRLLLQRMAEKVRLVTENEYLHERVTTEENLDGIIGSSANIQDVLRMISRL
                     KDTRTPVLISGESGTGKELAARAIHFRGSMAKTPFVAVDCGSLVPTLMESELFGYEKG
                     AFTGATKSKAGLFQSADGGTIFLDEIGELPQEMQAKLLRVLQEKEVRPVGSNEKISID
                     VRVIAATNRDLEASYRAGTFRKDLYFRLNVVTVHLPPLRERRSDIPVLVHHFLDRYAK
                     GSHIQVTAAAMKSLLHYDWPGNVRELENCIARAV"
     gene            complement(1112..1510)
                     /locus_tag="VGR03_06500"
     CDS             complement(1112..1510)
                     /locus_tag="VGR03_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV1993960.1"
                     /translation="MNAQQALWTGGPILLIEDEPSVIAFLRAALERRGYAVVNASSGA
                     EGLQRLSAGQYAGVISDIRMPGAVNGAEVHGWIQKNRPELKARIILISGDTANSDTQA
                     FLAQSGTPCIEKPFRVQQLISMVEKTFGKP"
     gene            complement(1507..3795)
                     /locus_tag="VGR03_06505"
     CDS             complement(1507..3795)
                     /locus_tag="VGR03_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV1993961.1"
                     /translation="MSRHAEELMELAVEAAQSRHLPDFLEQFALRATRMLDAIWGGVA
                     VYRGRETELHAMPHGGGAITEDSADWLISNARGSQNDVETRAIPKEIAAALPFPEEPG
                     SVVFVRIAASDNERLGTLCLIRNRKTLGADEKRLLHALASHAALSLENFRRFSQLERS
                     KRQWVEDIDAISDYIVVHDRAWNIVRTNRSLASHLGVPPVALVGEAMSTLRQIAETGS
                     DLPCPFCRDTQRSKEEYVAASAERIFLVSTSRTPGVSDDDTRTIHVLKDITDRREAER
                     KYRELFDSIQEGLFFATPDGRFLDVNDAMVRMLGYASREELLRADVSPHLYPVPEARE
                     KFLLALSERGVLRNYEETLRRKDGTLLHTLQNITAVRDARGRIAQIRGLMLDVTEQKT
                     FQSQLQRERDFNQKILNTTQSMILVLDTAGLISYANRRCYEAGYQEGELIGHRLVDWV
                     EASHREDFDAALETTAHGHQVENLELRVRRSDGSMGHFSISLSPMRDEQNTVNSVVVV
                     MTDITDAALLQAKLAHSEKMATIGRLVSGVAHEVNNPLAAILGFTDLLLENPEVPVSA
                     REDLQIILQETQRTKDIVQDLLSFARQRPVQRELVQVNSVLRQTIKLRSYDFASHGVE
                     VVEDFEETLAAALGDSQQLQQVFLNILNNAYDAVQDAGQRGLIKIHTRRHAEMIEVAI
                     SDNGTGIIDPQRIFDPFYTTKQAGKGTGLGLSICYGIVRAHGGEIQCWNNEAGAGSTF
                     VVRIPVATEAALAAAASKEAGR"
     gene            4238..4582
                     /locus_tag="VGR03_06510"
     CDS             4238..4582
                     /locus_tag="VGR03_06510"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEV1993962.1"
                     /translation="MRNSRWKKLAVATTAALVLSGASGFAQSGSTDESRRKVKTKTAP
                     LYPELARRMNVSGKVKIEVVITPDGRVKSTRVVGGHPLLVQACQDAVKEWKFLPAPEE
                     TTQVVEFEFHAN"
BASE COUNT          922 a         1547 c         1221 g         1093 t
ORIGIN      
        1 actgcccgcg cgatgcaatt ctccagttcg cggacgttcc ccggccagtc atagtgcagc
       61 aggctcttca tcgccgcagc cgtgacctgg atgtgcgagc ccttggcgta gcgatccagg
      121 aaatgatgaa ccagaaccgg aatgtccgac cgtcgctcgc gcaggggagg gaggtgcacg
      181 gtcaccacat tcagtcgaaa atacaaatcc tttcggaagg ttccggcgcg ataagaggct
      241 tccagatcgc gattcgtcgc cgcgatcacg cgaacatcga tgcttatctt ctcgttgctg
      301 cccaccgggc gcacttcttt ttcctgcaat acgcgcagta atttcgcctg catttcctgc
      361 ggcaattctc caatctcgtc taggaaaatc gtccctccat ccgctgactg gaagaggccg
      421 gctttcgatt tcgtcgcgcc ggtaaaagcg cccttctcgt atccgaacag ttcactctcc
      481 atgagcgttg gcaccagcga gccgcaatcg actgctacga aaggcgtctt ggccatggac
      541 ccgcggaagt gaattgcgcg ggcggccagc tcctttcccg tcccgctctc gccggagatc
      601 agtaccggcg tccgcgtatc tttcagacgg gaaatcatgc gcaggacgtc ctggatattc
      661 gcggaactgc cgatgatgcc gtcaaggttt tcttctgttg tcacgcgctc gtgcaagtac
      721 tcattttccg tgaccagacg caccttttcg gccatgcgct gcagcaacag ccgcatcttt
      781 tccacgcgaa acggcttttc gatatagtcg tacgcgccca gtttcatcgc atccactgcg
      841 gattcgatcg agccgtggcc ggtcatgatg gcgacttcgg tgtgcgggag aagcagtttc
      901 gtttgcttca gcaactccac cccggacatg ttgggtagtt tcaggtctgt gagaagcaga
      961 tccggagtat cggaatccag ccgcgccagc gctgtctccg cgctttccgc ttcgacgcag
     1021 acatatccca gagtgtttcc gatcgtcatg cacaggcggc ggatgctctg ttcgtcatcc
     1081 actaccaata gtcgtgtttt catctcgtcg ctcatggttt cccgaatgtc ttctccacca
     1141 ttgatatcaa ttgctgcacc cgaaaaggct tctcaatgca cggtgtcccg ctttgcgcca
     1201 ggaacgcctg cgtatcgctg tttgccgtgt ccccactgat cagaatgatg cgcgccttca
     1261 gttccgggcg gttcttctga atccacccgt gcacttccgc cccattcacc gcacccggca
     1321 ttcgaatgtc tgaaatcacg cctgcgtact gtccagccga aagccgttgc aatccctccg
     1381 ctcccgacga cgcattcacc actgcgtatc ccctgcgctc cagcgccgcc cgcagaaagg
     1441 cgattaccga cggctcatct tcgatgagca ggatcggccc gcccgtccac aaagcctgtt
     1501 gcgcgttcat cgtcccgctt ctttcgatgc ggcagccgca agcgcggcct ccgtcgccac
     1561 tggaatgcgc accacaaaag tgctccccgc tcctgcctca ttgttccagc actgaatttc
     1621 cccgccatgc gcccgcacga tgccgtaaca aatgctcagc ccgagccccg tgccctttcc
     1681 cgcctgcttg gttgtataaa aaggatcaaa aatgcgctgt ggatcgataa tccccgttcc
     1741 attgtcgctg atcgccactt caatcatctc tgcgtgccgc cgcgtgtgaa tcttgatgag
     1801 gccgcgctgc cccgcatcct gcaccgcatc gtacgcattg ttcaggatat tcagaaaaac
     1861 ttgctgtaat tgctgagagt cgcccaatgc cgccgccagc gtctcttcaa aatcttccac
     1921 tacctcaacg ccatggcttg cgaaatcata gctccgcagc ttgatcgtct gtctcaaaac
     1981 actattcacc tgcacgagtt cccgttgcac cggtctttgc cgcgcgaaac tcagcaaatc
     2041 ctgcacgata tctttcgttc gctgtgtctc ctggaggatg atctgcaaat cttcccgcgc
     2101 cgacactggc acctcgggat tctccagcaa aagatccgtg aaacccagaa tcgccgcaag
     2161 cgggttattg acttcatgcg ccacgccgga aaccagccgt ccaatcgtgg ccattttttc
     2221 gctatgggcc agttttgctt gcagcaacgc cgcgtctgtg atgtccgtca tcaccaccac
     2281 gacgctgttc accgtattct gctcatctct catcgggctc aggctgatcg agaaatgccc
     2341 catcgacccg tcgctccgcc gcacccgcag ttccaggttc tccacttgat gcccgtgcgc
     2401 cgtcgtttcc agcgccgcat cgaaatcttc ccggtgcgaa gcctccaccc agtccaccag
     2461 ccggtgcccg atcaattcgc cttcctggta gcccgcctca tagcagcgcc ggttggcgta
     2521 gctgatcagc cccgccgtat ccagcaccag aatcatgctc tgcgtcgtat tcaaaatctt
     2581 ttgattgaaa tcgcgttccc gctgcagttg cgattgaaaa gtcttctgct ccgtaacgtc
     2641 cagcatcagc ccacgaatct gcgcgatgcg tccgcgggca tcgcgcactg cggtgatgtt
     2701 ttgcagcgtg tgcagcagcg ttccatcttt gcgccgcagc gtctcttcgt agttgcgcaa
     2761 cactcctctt tccgaaaggg ccagcaggaa tttttcgcgt gcttccggca ccggatacag
     2821 atgcgggctc acatccgccc tcagcaactc ctcccgcgac gcgtacccca gcatccgcac
     2881 catcgcgtca ttcacgtcaa ggaatctccc gtccggcgtg gcgaaaaaca atccttcctg
     2941 aatgctgtca aacagctcgc gatacttccg ttccgcctcc cgccgatcgg taatgtcctt
     3001 cagaacatgg atggtccgcg tatcgtcatc agacacgccc ggcgttcgcg aagtcgaaac
     3061 cagaaagatg cgctctgccg acgcggccac gtactcttcc ttcgatcgct gcgtatcccg
     3121 gcagaacggg cagggtagat cgcttcctgt ttccgcgatc tgcctcaagg tgctcatggc
     3181 ctcgccaacc agggccacgg ggggcactcc aagatgggac gcgagcgacc ggttcgttcg
     3241 aacgatattc cacgcgcggt cgtgcaccac aatgtaatcg ctgatcgcgt cgatgtcctc
     3301 cacccactgc cgtttggacc gctccaattg cgagaatcgc cggaaattct ccagcgagag
     3361 cgccgcatga ctcgccagcg catgcagcaa tcgtttctca tccgcgccca gggtctttcg
     3421 gttccggatc aggcagagcg ttcccagcct ttcattgtcg ctcgccgcga ttcgcacgaa
     3481 gacaaccgac cccggctctt caggaaaggg caaggccgcg gcaatctcct tgggtatcgc
     3541 tcgggtttcc acatcgttct ggctgccccg ggcgttcgaa atgagccagt ctgcgctatc
     3601 ttcggttata gcgccgccgc cgtgcggcat ggcgtgaagt tccgtttcgc gcccacggta
     3661 gacggccacg cctccccaga tggcatctag cattcgcgtc gcacgcagtg caaactgttc
     3721 cagaaaatct ggcagatgcc gagactgcgc cgcctccacg gccagttcca tcaattcttc
     3781 agcgtgcctc gacatggact ggcggggctc cgcccttccg cgctggggcg tgggatgacc
     3841 tgacgttctt gtctttatta tccgcatcga ccagaagaga gcaccacgcg tctcaagttg
     3901 cgaggcgctg ctcctcccca accctaccgg ggagtctcaa tgtgagtcaa ttgccgggca
     3961 gcttcgtgta gcgaaatcgc ctatagcagc agtgcttcct tttctctccg cgtctcagcc
     4021 tgagacctca gcctctcgag tcccgcccgc ctgagacaaa tggtactccc gtgccacatt
     4081 gcctccagct cctaaatttt ccataacttg ctgttctcct gttacttacc gatgctcgac
     4141 cggctccgat tcgattattc ctgcacctct ccgccatggc atcccacgtg ccttctccta
     4201 tgcggggtat cttccaatcc catgaggagt agtgtcgatg agaaatagta ggtggaagaa
     4261 gttggctgtg gctacaactg cggcgctcgt tttgtcgggc gcttctggat ttgcccagtc
     4321 tggttcgacg gacgaatcca ggcgcaaggt caaaaccaaa acggcaccct tgtatccgga
     4381 acttgccaga cgcatgaacg tttccggcaa agtcaaaatc gaggttgtca ttacgccgga
     4441 cggacgtgtg aaaagcacgc gggtcgtcgg gggacacccc ttgctcgttc aggcttgcca
     4501 ggacgccgtg aaagaatgga agtttttgcc tgcgccggaa gagaccacgc aggttgttga
     4561 atttgagttc cacgctaatt aggggaatca cttgaggaag tgttgttccg gcagaagcgg
     4621 ccatctccac ggcgccctgc tgaagtgagc ggaggcattt tgcaatgacc atcggaaaaa
     4681 agatttacgc gggtttcggg ctgatcctcg gcattctctc gctgctattc atcgtgaata
     4741 tcatcaccgg catcaaggaa agctcgacgc gcaaggatgc cgg
//
LOCUS       DASYVO010000194        17106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_272843, whole genome shotgun sequence.
ACCESSION   DASYVO010000194 DASYVO010000000
VERSION     DASYVO010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 17106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17106
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272843"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VGR03_06515"
     CDS             complement(<1..379)
                     /locus_tag="VGR03_06515"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1993963.1"
                     /translation="MADTPLQVTGRQTKQEEAVSVIEVLGDAAKRTVASVQDYAVLSG
                     HAFANIFTSPHYFQDTLEQMDFIGAGSLPIILLSGFFIGAVMVLQTGSQFIRFGQTSL
                     TGDVVAIALVRELGPAFTGLLVTG"
     gene            786..3161
                     /locus_tag="VGR03_06520"
     CDS             786..3161
                     /locus_tag="VGR03_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1993964.1"
                     /translation="MGGFWQDLRYAFRALRKSPWFATLAVVTLALGIAVNTSIFSIVN
                     GFLLRPMPVPHPEQLAVLSLQQAGDKSLQSFSYPDYLDLRGQSTSFSDVIAYRVTLAG
                     LTADNRGDHCIATRVTGNYFSILGVQPALGRLILPTEGQTPGADPILVLGYSFWQKRF
                     SGDKNIIGKHVEINRHPMTIVGVTPKEFHGTYSIIDSDLYIPLSADTSTKDEKQVQDT
                     WTHRSERSLSLMARLKPGTNLKQAQTSLNVVGQRIAEQHPDTDKGITIRAFPEQLARP
                     EPDPDNTLPSVAAAFMALAALVLLVACFNVTNVLLVRATARQREMAIRAALGAGRMRL
                     VRQYLTESLLLAFLGAGGGMLLAYWAMRFLSSIPLGTDLPLQLKFLPDARVYFFALGA
                     ALVTGVIVGVFPALRVAQVAGSLVLLIVAGLFIRSLGKAQNIYLGFNPANVLDFSLDV
                     QQIGYQEVQGRAFYRELESRLRTLPGVVSVAQAFSVPMGVMSSFDSVNVEGHPVEAGQ
                     QPPSVQYNMVTPGYFDTLRIPVHRGRTFTDADEEKTLAVAIVNQTMAKKFWPDQDPLG
                     KRFSTKGASGPFIEVVGVVQDGKYKGVVEDPQPHFYLPLNQAYLPLRTFHVRASVPPE
                     SLTTQVQSQIRQLAPTLAISELQTLDQALQGLNGFLFFRLGAQLTGTMGLLGLILAVV
                     GVYSVASYAATQRTQEIGIRMAIGATPRDTLKMVLRQGFGIVGIGLLAGLVAAFAGTR
                     LLADIFYGVTPSDPVTYAAVATLLLAVTLLACWIPARRATRVSPIVALRFE"
     gene            complement(3180..3413)
                     /locus_tag="VGR03_06525"
     CDS             complement(3180..3413)
                     /locus_tag="VGR03_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1993965.1"
                     /translation="MVLGQGLRITLIGVAIGLAAAFGLTRLITTLIYGVGASDPITFA
                     SVAILLSGVALLACYIPARRAMRVDPMVALRYE"
     gene            complement(3404..5617)
                     /locus_tag="VGR03_06530"
     CDS             complement(3404..5617)
                     /locus_tag="VGR03_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1993966.1"
                     /translation="MMSFWQDVRYGLRMLSKNPGFTAIAILTLALGIGANTALFSVVN
                     GVLLNPLPFPNPDELVAVYAKSPTFQESSIAYPNFLDWQKDNHSFAALSAFRSDDYNM
                     TGAGEPERVHIHMISAEFLPALGMKPLLGRTFRPEEDKAGAGPVTILSDGLWKRKFGL
                     AQDVLGKSITLNGKAYAIVGVASGRIAGLSPTDLFIPIGQWNDPSFRDRRISMGMNSI
                     GRLKHGATIDQARADMNRIGENLAVAYPEADKGTGITLVPLKTDVVGDVKGILLVLLG
                     AVSFVLLIACANVANLLLARSTGRAREFAIRAALGASPARVIRQLLTESVLLGIAGGC
                     IGLLLAKLGVRVLLATLAGNMPRSDEISLDARVLFYTFGISVLTGIVFGLIPALNMLR
                     PDTHETLKEGGRGSSGARHRTQSVFIVVEMAMAVVLLIGAGLMIRTLSALGNINPGFD
                     PRKVLTFSISSTSNSAFNPDQLRAMYRETLRQLQSVRGVEAVSMMGGSLPMTGDSEIP
                     FWLEGQPKPANDNDMPFALFYLVNPGYHQAMRIPVERGRTFTEQDNEHGPAVALIDAT
                     FARKYFPNQDPIGKHLNLGLFDTQPEIVGVVGHVEHWGLGSREHQNLQAQLYLPVWQV
                     PDRFWPLLANGSGYVARTAGTAGDITGAIREAAEKVEGSAVVYGIRPMEEIVARSIST
                     QRLTMFLLSIFSALALVLSAVGIYGVISYLTGQRTHEIGVRGRLVHPAETSCVWC"
     gene            5766..6134
                     /locus_tag="VGR03_06535"
     CDS             5766..6134
                     /locus_tag="VGR03_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993967.1"
                     /translation="MTVGLALRWGCERSGRGMSAELDAGRAGLEVVFVVDGGEGAEEQ
                     VAGVGHDGGAARGDAVVGFEKEEPRKKTVDVRGGGEFGELASEVAGEIVGVAFFPAKL
                     GVAETKMRFRVEDAKAAAAA"
     gene            complement(6765..7046)
                     /locus_tag="VGR03_06540"
     CDS             complement(6765..7046)
                     /locus_tag="VGR03_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993968.1"
                     /translation="MGSPQTKNAPMHFKTMVQLDVPQGRNGKHKLIVTTILKDLDSLK
                     AGSALKVPLAELVESKEKVRSALNRATRKGGRKVATATDAAFLYVWNVN"
     gene            7294..7455
                     /locus_tag="VGR03_06545"
     CDS             7294..7455
                     /locus_tag="VGR03_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993969.1"
                     /translation="MLKLKTHFEQVPLEMVKQIVEKELQREMASEQARGTKKKKLEED
                     LLEASTVNG"
     gene            7682..8377
                     /locus_tag="VGR03_06550"
     CDS             7682..8377
                     /locus_tag="VGR03_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV1993970.1"
                     /translation="MQVFASSSNIRSKRTSKQKLAFDAQAFLDSAGVARRVVEYRRSQ
                     KIYSQGDTAASVMYVQKGGVKLSVVNEVGKEAVVAILGPGDFFGEGGMAGQSVRMGTA
                     TAVTPTTLLVIEKNEMIRVLHAEHAFSDRFVSYMLSRNIRIEEDLIDQLFNSSEKRLA
                     RTILLLARYGKEEQPHGVLPKVSQETLAEMIGTTRSRVNFFMNKFRKLGFIQYNNGGL
                     QVHKSLLSVVLHE"
     gene            complement(8580..9149)
                     /locus_tag="VGR03_06555"
     CDS             complement(8580..9149)
                     /locus_tag="VGR03_06555"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1993971.1"
                     /translation="MKNIDRRTLLYAFGIGSATLMLPGQAAASGIVAVAKPGENRFAF
                     ASATQAKTSPCKVSSEDSGGACTVLELNALPRSGPFLHVHNREDEWYYVLSGEFIFRA
                     GSEEYNLLAGGSIWLPRGIPHVWANTATADGKLILVCQPGGFEKFFDEIGNVPMGKKN
                     ADTMKGLMAKYGMEMLGPPIFTSSWMQQH"
     gene            9635..10804
                     /gene="aroC"
                     /locus_tag="VGR03_06560"
     CDS             9635..10804
                     /gene="aroC"
                     /locus_tag="VGR03_06560"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV1993972.1"
                     /translation="MLRFYTAGESHGQALLAFISGLPAQMPIDVKFINHELRRRQLGY
                     GRGGRQKIEKDTAEIFAGVRHGKTIGAPIALRIENRDWANWEKILPVEAGEDDPGGRK
                     LVAPRPGHADLAGSQKFNYHDARYILERASARETAARVAAGAFAKLLLREFDTEIASH
                     TIQVGHVRLEKPATWKKIHAVSKDLDSPLRCIDAAVQEEMKAEVDAVLKAGDTVGGIF
                     EIVAHNVPVGLGSHAQWDEKLDGRLAQALMSVQAVKAVEIGGGVAGAGSFGSEVQDEI
                     FYEKSARRFRRATNRAGGVEGGITNGEDVVVRGYLKPISTLRQPLHTTDMVTKEPVKA
                     AYERSDWCVVPAGAVAGEAMVALVLADAFLQKFGGDSLAEMRRNFDNYARQIDEF"
     gene            10779..11366
                     /gene="def"
                     /locus_tag="VGR03_06565"
     CDS             10779..11366
                     /gene="def"
                     /locus_tag="VGR03_06565"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1993973.1"
                     /translation="MPDKSTSSEAANATGTETAQALSAARKIYPIVKYGDAILEKPGL
                     AIRKFDAELEQLAEDMFASMYAAQGVGLAAPQIGTSLRIAVVDVTGGKNPEAKIVLAN
                     PEIIHAEGEVREEEGCLSVPGFRGYVLRPQFVTIKAQNAKGETFEIRGENLLARAFCH
                     EIDHLNGILFLQHLSMLKRDLIKRKIKKLRKKGEW"
     gene            11407..12360
                     /gene="fmt"
                     /locus_tag="VGR03_06570"
     CDS             11407..12360
                     /gene="fmt"
                     /locus_tag="VGR03_06570"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV1993974.1"
                     /translation="MRIVFCGTPSFAVPTLKYLLAQSEFEIVWVITQPDRPRGRGQEV
                     SFSPVKEAALAANLPVHQPEKIRAPETEALLQKLAPDCVVIIAYGQIIPARLLPIPKL
                     GWINLHASLLPKCRGAAPINWAIVNGETRTGVTTMRIDAGMDTGEMLLQREIEIGPKE
                     TAPELAARMSESGAPLMAETLRGLAAGTISPKAQNHAEASYAPMLKKEDGRIDWKQPA
                     MEIYNRMRGFAPWPGAYTTFRGQSCHVWGEPVSKEGGAGLPGGAAPGTLFGEKNELLV
                     WCGDATVLCVRLVQVEGRKPVKASDFANGARLKSEERFGDS"
     gene            13372..14274
                     /locus_tag="VGR03_06575"
                     /pseudo
     CDS             13372..14274
                     /locus_tag="VGR03_06575"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
     gene            14271..15989
                     /locus_tag="VGR03_06580"
     CDS             14271..15989
                     /locus_tag="VGR03_06580"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEV1993975.1"
                     /translation="MKILHRPKALRVYKQWAGIVIAALLAMNATPNRAATEGAKAAPD
                     AAMVAAHAAAKGDGLLEALLTELDRSKAQLKMDQVQAPYYVEYRVNEVEDIGAEAAYG
                     ALRESQHIHVRVLRVVVRIGDYRQDSYFGRGQGESSILPLDNDPIALRHQIWLATDEA
                     YKAAGQALAEKQAAMKQFSVDPNPVDDFAKAPQMIAVEPTASLKIDEAGWKKTLEDVT
                     SLYKQYPDVQSVTASARFSAINEYLVNSEGTVTRSGKTTYSVQLSGTAQAADGMRLSR
                     SPAFMVARPEELPTHDALMGEAKKMLETVVALRQAPIVEEEYRGPVLFAADAADDIVA
                     SLIGQNVLGQKPQLGKPNRTTGAFATSYKARVLPNFLSVVDDPTMKDFQGKSLVGSYA
                     VDSEGVKAQAVNTIESGMMANYLIGRVPIRDFATSNGHGRAGAGSFPGPSLGVLLVKS
                     SEAQAPEELKKKIIQMVTEQGKAYGYRVETLGPGNSPRLLYRVYAKDGHEELVRGAVF
                     SELDIRALRNDLIAVGNDPLVSNRAGGAPTTIISPSLLFGELEVKRADTSKDKLPDYP
                     APPLKK"
     gene            complement(16585..>17106)
                     /locus_tag="VGR03_06585"
     CDS             complement(16585..>17106)
                     /locus_tag="VGR03_06585"
                     /inference="COORDINATES: protein motif:HMM:NF042813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIL restriction-modification enzyme MmeI"
                     /protein_id="HEV1993976.1"
                     /translation="IPRHSSERRKYIPIGYVKPEILAGDSCLIISNATPYHFGILSSV
                     MHMAWVRLVCGRLESRYRYSSNLVYNNYPWPEQPKAKQREAVQAAAQAVLDARKVFPE
                     ATLADLYDPLAMPPVLVKTHADLDRAVDLCYRPQPFQNDRQRVEHLFALYEKLTAPLI
                     APAKKGRRKAHQN"
BASE COUNT         3818 a         4580 c         5298 g         3410 t
ORIGIN      
        1 ggcccgtcac cagcaaaccc gtaaatgccg gccccagttc tcgcaccaga gcaatcgcca
       61 caacgtcgcc ggtcagcgaa gtctgtccaa aacggatgaa ttgcgacccc gtttgcagaa
      121 ccatgaccgc gccgataaag aaccccgaca atagaatgat cggcagggac ccggcgccga
      181 tgaaatccat ctgttccaga gtgtcttgaa agtagtgcgg acttgtgaag atgttggcaa
      241 acgcgtgccc ggagagaact gcgtagtcct gaacggaggc caccgttcgt ttggccgcat
      301 cccccaatac ttcaatcacg gagacggctt cttcctgttt cgtctggcgg ccggtgacct
      361 gaagaggagt gtctgccacg agtacctgtt cgcgttggct tcccgcgacg aacttctcgg
      421 cacaacaaca gcaacgaaag cctaaccgtg cggcaaggac acgtcaagtc actgatttgg
      481 cttgtcgctg caaaaggaga atgtccggtt tgtgcaaagt agaaatgtcc gctttcatgg
      541 ttggccgaag acacttgggc caaccgcctt gaatttttgg aaggtcggcc tccaaggaca
      601 ttatatctgc agcctgccgc ctgtccggtt ccgcaacaat gtcgtcccgg aatcgcgcag
      661 ttattttcgc tgccaccctc gcaaactgcg cgtaacctgt aagatttcag taagatagcc
      721 aataccggcg gataggaatc atttttgcaa attaccctta agggataatc cagcgaggtg
      781 ccgcaatggg aggtttttgg caggatctgc gctatgcgtt tcgcgcgctt cggaagtctc
      841 cttggttcgc caccctcgcc gtcgtgaccc tcgccctcgg catcgccgtc aacacctcca
      901 tcttcagcat agtcaacgga ttcctcttgc ggccgatgcc ggtgccccat cccgagcagc
      961 ttgccgtcct cagcttgcaa caagccggcg ataaatcact gcaaagcttt tcttaccccg
     1021 attatctgga tctccgcggc caatccacct ccttcagcga cgtcattgca taccgcgtca
     1081 cgttggccgg cctcaccgcc gacaatcgcg gcgaccattg catcgccact cgcgttaccg
     1141 ggaattattt ttcgatactc ggcgtccagc ccgcactcgg ccgtctgatc ctccccacgg
     1201 aaggacaaac tcccggagcc gatcccatcc ttgtcctcgg ctattcgttt tggcaaaaaa
     1261 gattctccgg tgacaagaac atcattggaa aacacgttga aatcaatcgc catccgatga
     1321 ccatcgtcgg ggtaactccc aaggaatttc acggcactta ttccatcatc gattcggacc
     1381 tctacatccc tctcagcgca gacaccagca caaaagacga aaaacaagtc caggacacct
     1441 ggactcaccg ctccgaacgc tcgctttccc tgatggccag gctcaagccc ggaacaaatt
     1501 tgaaacaggc tcaaacttcc ctgaatgttg tcggccaacg catcgccgag cagcatcccg
     1561 acaccgacaa gggcatcacc attcgcgctt ttcccgaaca actcgcccgt cccgaacccg
     1621 atcccgacaa cactctcccc tccgtcgcgg ccgccttcat ggctcttgcc gctctcgttc
     1681 ttctcgtggc ttgcttcaac gtcaccaacg ttttgttggt tcgcgccacc gctcgccagc
     1741 gcgaaatggc cattcgcgcc gccctcggcg ccggccgcat gcgactcgtt aggcaatacc
     1801 tgacggaaag tctgcttcta gcttttctcg gcgcgggcgg cggcatgctg ctcgcctatt
     1861 gggccatgcg attccttagt tccattcccc tcggcacgga cttgcccctt cagttgaagt
     1921 tcctccccga cgcacgggtc tatttctttg cgctcggtgc ggctctggtc accggcgtga
     1981 tcgtcggtgt cttcccggcg ttgcgcgtcg cacaggtagc aggctcgctg gtgctgctca
     2041 ttgtcgccgg cctcttcatc cgcagcctcg gcaaagcgca gaatatttat ctgggcttca
     2101 atcccgccaa cgttctcgat ttctctctcg acgttcaaca aatcggttat caggaagtcc
     2161 aaggccgcgc cttttatcgc gaactggaat cgcgcctccg caccctacca ggcgtcgtct
     2221 ccgtcgcgca ggctttctcc gttcccatgg gcgtcatgag cagcttcgac tccgtgaacg
     2281 tcgaaggtca tcctgttgaa gcaggccagc aacctcccag cgtgcagtac aacatggtca
     2341 cgcccggata tttcgatacc ctgcgcattc ccgtgcatcg cgggcggacc ttcaccgatg
     2401 ccgacgagga gaagaccctc gccgtggcca tcgtcaatca gaccatggcc aaaaaattct
     2461 ggcccgatca ggatcccctc ggtaaacgct tcagcaccaa aggcgcttcc ggacccttca
     2521 ttgaagttgt cggcgttgtg caagacggca agtacaaagg ggttgtcgaa gatcctcagc
     2581 cgcacttcta tctgcctcta aatcaggctt acttgccgct gcgcacgttc catgttcgtg
     2641 cttcggttcc cccggaaagt ctcacgacac aggtgcagtc gcaaatccgc caactcgctc
     2701 ccacccttgc catctccgaa ttgcagactc tggatcaggc gctccaagga ttaaacggct
     2761 tcttgttttt ccgcctcggc gcgcaattga ccggcacgat gggcctgcta ggactcatcc
     2821 tcgccgtggt tggtgtctac agcgttgctt cctatgccgc gactcagcgc actcaggaga
     2881 tcggcatacg catggcgata ggagcgacgc cgcgcgacac tctaaaaatg gtcctgcgcc
     2941 agggcttcgg catcgtcggg atcggcctgc ttgccggact ggttgccgcg ttcgccggaa
     3001 cacgccttct tgcagatatt ttctacgggg tcacgcccag cgaccctgta acctatgcag
     3061 ccgtcgccac gcttctgctc gccgtgacgt tgctggcctg ctggattccg gcgcgccgcg
     3121 cgacgcgcgt cagccccatt gtcgccctgc gctttgaata gccaataccg cgcgcatttt
     3181 cattcgtaac gcagggcgac catcggatcc acgcgcatgg cccggcgagc ggggatgtag
     3241 caggccaaca gcgccacacc cgagagtaga atggcgacgc tggcaaacgt gattggatcg
     3301 gacgcgccga ctccgtagat caatgtggta atgagccgcg tgaggccaaa cgcggcggcg
     3361 agcccgatgg cgacgccgat caaggtaatt cttaatccct gtcctaacac catacgcaag
     3421 acgtctctgc tggatgcacc aagcgccctc gaaccccaat ctcgtgcgtg cgctggccag
     3481 tcaaatagga aatcacgcca taaatgccga cagcagaaag aacgagggcg agcgcggaaa
     3541 agatgctaag gagaaacatt gtcagccgct gcgttgaaat cgatcgggcg acgatttcct
     3601 ccatgggcct gatgccatag acgacagcgc tgccctccac tttttcggca gcttcacgaa
     3661 tggcgccagt gatgtctcca gcagttcctg cggtgcgggc gacgtatccg ctgccgtttg
     3721 caagtagcgg ccagaatcgg tctggcacct gccaaaccgg cagatagagt tgggcctgaa
     3781 gattctggtg ctctcgcgaa cccaggcccc agtgttcgac gtggccgaca acgccaacga
     3841 tctccggctg agtatcgaac agtccgaggt tgaggtgttt tccgatggga tcctgattcg
     3901 gaaaatactt gcgcgcgaaa gtggcgtcga tcagcgccac cgcaggtccg tgctcgttat
     3961 cctgctccgt gaacgtgcgg ccacgctcga cagggattcg catggcctgg tggtagcctg
     4021 gatttacaag ataaaacagc gcgaaaggca tgtcgttgtc atttgccggc ttcggctggc
     4081 cttccagcca gaaaggaatc tcggaatctc cagtcatcgg cagcgagccg cccatcatgg
     4141 agaccgcttc cacaccgcga acgctctgca gttgccgcag ggtctcgcgg tacatggcgc
     4201 ggagttggtc ggggttgaag gccgaattgg aagtcgagga gatgctgaag gtgaggactt
     4261 tgcgcggatc gaaacccgga ttgatgttgc cgagcgccga gagcgtgcga atcatcagcc
     4321 ctgcgccgat gagaagtacc acagccatgg ccatttcgac gacaatgaaa acgctttgcg
     4381 tgcgatgacg cgcgccgctc gagccgcggc cgccttcctt taacgtttcg tgagtgtcgg
     4441 gccgcaacat gttgagcgcg ggaatcagcc cgaacacgat accggtcaag acggagatgc
     4501 cgaaggtgta gaagagcacg cgggcatcga gcgaaatttc gtccgaacgc ggcatattac
     4561 cggccagagt tgcgagcagg acgcgcacgc ccaactttgc caggaggaga ccgatgcagc
     4621 cgccggcaat cccgaggagg acgctctcgg taagcaactg gcggatcacg cgcgcgggac
     4681 tggcgccaag cgcggcgcga atcgcaaatt cgcgggcgcg gcccgtcgag cgcgcaagaa
     4741 gcaaattggc gacgttggcg cacgcgatca agagcacgaa actgacggct ccgagcagca
     4801 cgagcagaat tcctttcacg tcaccgacga catcggtctt gagcgggacc aaagtgatgc
     4861 ccgtgccctt gtcggcctcg ggataggcga cagccaagtt ctcgccgatg cggttcatat
     4921 cggcgcgggc ctgatcgatg gtcgcgccat gcttcaacct gccgatcgaa ttcatgccca
     4981 tgctgatgcg ccggtcgcga aaggagggat cgttccactg accgatggga atgaaaaggt
     5041 cggtggggct cagccccgca atgcggccag acgcgactcc tacgatagcg taagccttgc
     5101 cgttcagcgt gatgctcttc cccaaaacat cctgcgccag accaaacttg cgcttccaaa
     5161 gcccatcgct gaggatcgtc accgggcccg cgcccgcctt gtcctcttcg gggcgaaacg
     5221 tgcggccgag cagaggcttc atgcccagag ccggcaaaaa ctccgcggaa atcatgtgaa
     5281 tatgaacgcg ctcgggctcg ccggcgccgg tcatgttgta gtcgtcggag cggaaagcgc
     5341 tcagcgcagc aaaggaatgg ttgtctttct gccagtcgag gaagttcgga taagcgatgg
     5401 agctttcctg aaacgtcggg gacttcgcgt agacagcgac cagttcgtcg ggattcggaa
     5461 acggcagcgg attgaggagc acgccgttga cgacggagaa aagggcggtg ttcgcgccga
     5521 ttcccaaggc aagcgtcagg atggcgatcg ccgtgaatcc aggattcttc gacagcatgc
     5581 gcaatccata ccggacgtct tgccagaagc tcatcattgg acaccccttt caggtacacc
     5641 ccagcggctg cagggcttct ggagttagaa acggaattga cccgtttttt gtttcctgaa
     5701 agtgggaacg gaatcgtcat gctggagttt tcccggggtc gaggttcacg gcggggatat
     5761 cccagatgac ggtggggctg gcgttacgat ggggctgcga acggtcgggg cgcggcatgt
     5821 cggccgagct cgacgcgggg agggccggcc tggaggtcgt tttcgtagtc gatggcgggg
     5881 agggtgcgga ggagcaagtt gcaggtgtag gccatgacgg cggcgcggcg cggggcgatg
     5941 cggtcgtggg cttcgagaag gaggagccgc gaaagaaaac tgttgatgtt cgcggcggag
     6001 gtgaattcgg tgagttggcc agcgaggtcg caggcgagat cgtcggcgtg gcgttttttc
     6061 cggcgaagct gggcgtggcg gaaacaaaga tgagattccg cgttgaggac gcgaaggcgg
     6121 cagcggcggc ctgagacagt tcggtgacgc cagagcgata gttcgttgac ggaattggag
     6181 ttatcggttt tctcggtcat attgatcctt atgaagggtg tgccgttgta ggccacccct
     6241 gttgtgaagg caaagagtct gtaaatggca gaaaagaaaa gagatgcaga tgccgctgtg
     6301 gggagtagac ggaaaaaagt tcggaagtgg ttgagaaaaa aggggatagg aagatgcgaa
     6361 ggccgacaac tgtcagggtt gcaggattaa cgatgaacaa gacctggtcg gttttggcga
     6421 gcactagcag ggcagtttgc ggcgtctctg cggtgagcag ccccgcgccg ctgcaaagga
     6481 gagcaaagag aacaagtgca aaagatcgtc cggacgttat agattttccg cgcctttcag
     6541 ccgtgagcgg taaagccaat aacaatgtcg aagtttgcgg cgagttcgtc gaatcgagaa
     6601 gagtttcccg accatggtat cactgctagc aacctagtgc ctggtaaacg caccgatcgg
     6661 aaactggcgc cccatgtccg gtaatccgat ttatcaggag ttagcgcggg agccgccgct
     6721 gccagggcgc atatctggtg ggaaaggcca gatggttctc gctactagtt cacgttccac
     6781 acgtacaaga aggcggcgtc ggtggcggtc gcaaccttgc gtccaccttt ccgagttgca
     6841 cgatttagcg cggaacgaac tttttctttt gattccacca attcagccag tggcacctta
     6901 agcgcagaac cagctttcaa actgtcgagg tccttgagga ttgtggtgac aattagtttg
     6961 tgctttccgt ttcgaccttg cggcacatcc aattgcacca ttgtcttgaa gtgcattggt
     7021 gcgtttttgg tctgagggct tcccatatcg tcctcccagc ggacaccacg ctaaacaaac
     7081 gtaacgttat cgtatctcat cgtagtgagt cctgcaagtg aaataaacgg gccgtaaagc
     7141 ccagcgtcgg cccggctcgg agcgacacaa actcgccgcg ctccgttaag cgaggagctt
     7201 agcccagact aaacgaaagt tcgggctcta aaagatatga cccgtacgac ttccggcctg
     7261 atagcgtcca tatgattcga aggagcgatt gtaatgctca agctgaaaac acatttcgag
     7321 caggtaccac tggagatggt caagcagatt gttgaaaaag aactccagcg agaaatggcg
     7381 agcgaacagg cccgagggac taagaagaaa aagttggaag aggacctttt ggaagcaagc
     7441 acggtcaatg ggtgaggtgg aaaatcatga ctcttccgtt cgacactttc cagagagcaa
     7501 ccagcggcaa ggtcctctgg cttgaatcag cggtaactct cgaggacgtc aaggcacggg
     7561 tccaagaatt agcggcgcgt tcacccggtg agtacctcct cttgaaccaa cgtacgggat
     7621 acaaactcaa tagcaagttc gatggcgcgg acggagcacc aggccgctga tcggaggcgt
     7681 tatgcaagta ttcgcgagtt caagcaacat tagatccaag agaacatcca agcagaagct
     7741 cgctttcgac gcccaagctt ttctcgattc ggccggtgtg gccagaagag ttgtggaata
     7801 tcgaagatcc cagaagatct actctcaagg ggataccgct gcgagtgtga tgtacgttca
     7861 aaagggcggt gtgaagctct ctgttgttaa tgaggtcggc aaagaagcag tcgtggcgat
     7921 tctgggacca ggtgactttt ttggtgaggg gggcatggca ggtcagtctg ttcgaatggg
     7981 gacagcgacc gcggttacgc ccacgacctt actcgttatt gaaaaaaacg agatgattcg
     8041 tgtgctccac gctgaacatg ctttttccga ccgcttcgtg tcttatatgc tttcacggaa
     8101 catccgaatc gaggaggatt tgatcgatca gctttttaac tccagcgaga aacgactggc
     8161 ccgcactatt ctattgcttg cccgttacgg taaagaagag cagccccacg gagtactccc
     8221 caaagtatcg caagaaacgc tggcggaaat gattggcaca acgcggtcgc gggtgaattt
     8281 tttcatgaac aaattcagga aactcggttt cattcaatac aacaatggcg gacttcaagt
     8341 ccacaaatct ctcctaagtg ttgtcctgca cgagtaagcc ccactttcgc gcttatcgga
     8401 aagcggcata cttccagatc gctgttggta acagggttta ccagctaatt ggacccaact
     8461 cctgaataac gcgcttaccg gagaagtgaa atcgctgacg caagctggca ggcagatgtc
     8521 tggcaatccg actactcagg agttagccga gcagcaggat ggagtcggtg ctgtgatccc
     8581 tagtgctgtt gcatccagga ggaagtaaaa attggcggtc ctaacatctc cattccatat
     8641 ttcgccatca atcccttcat cgtgtcagcg ttctttttgc ccatcggcac gttcccaatc
     8701 tcgtcaaaaa acttctcaaa tccaccgggt tggcagacca agatcaactt gccatcggct
     8761 gtagccgtgt tcgcccacac gtgggggatg ccacggggca accagatgct cccgccagcc
     8821 aaaagattgt attcctcgct gcccgcccta aaaatgaatt cgccggagag aacgtaatac
     8881 cactcgtctt cgcgattatg aacatgcagg aatgggccag agcgcggcag tgcgttgagc
     8941 tccaagaccg tacacgcccc tccgctatct tcgctggata ccttgcacgg ggaagtcttg
     9001 gcctgagtgg cgcttgcgaa tgcgaagcga ttctcgccag gcttggctac ggcaactatt
     9061 ccgctggcag ccgcctgacc cggtagcatc aaggtcgcag atccgattcc gaacgcatag
     9121 agtaaggtgc gacggtcgat atttttcatg gcgcgaacta tatccgcgta aatgacaaat
     9181 cacaaccggc aggaggattc acaccccaga aaccttggct atggaagtcc ggctacgaat
     9241 cgcgactaac tcctgattaa agcggtaatc ggaaactagc gcccaatgtc cggtaatccg
     9301 acgttgcgct gtggatattc accgagccca tacaacgagc aaacctgcag ccgacctggc
     9361 gcaggagctt tgatgcggcg atggtcaagc ttgtgaacga cgttagaaac ctggctggac
     9421 aacccgcggc tgcacgggtt agcgcgaaga agtaaatcat ccggcaaagg atcaaggact
     9481 tccctcttct tctaagagct gcttgaaatt catcgggttt agcgcagcag agcctgggga
     9541 cgcgctggac gcagagggcg gcggcggcta cactgtttgt attccaattg ttttgagtca
     9601 aatacgcgca aaatcgtgcg aggaatgaac ggggatgctt cgcttttaca ccgcgggtga
     9661 gtcgcatgga caggcgctgc tggcgtttat ctccggactg cctgcacaga tgccgattga
     9721 tgtgaagttt atcaatcacg aactgcggcg gcggcaactg ggttatgggc gcggcgggcg
     9781 gcagaagatt gagaaggaca ctgcggagat ttttgcggga gtgcgccacg gaaagacgat
     9841 tggggcgccg attgcgttgc ggatagagaa tcgagattgg gcaaactggg aaaaaatcct
     9901 gccggtggaa gcgggggaag acgatcctgg aggacgaaag ctcgtcgctc cgcgcccggg
     9961 ccatgcggat cttgcgggat cgcagaagtt caactaccac gatgcgcggt acattctgga
    10021 acgagcctcc gcgcgcgaga ccgctgcgcg cgtggcggcg ggggcgtttg caaaactgct
    10081 gttgcgagag ttcgatacgg agatcgccag tcacacgatt caggtggggc acgtacgcct
    10141 ggaaaaaccg gccacttgga agaagattca cgcggtgagc aaggacctgg attcgccttt
    10201 gcggtgtatt gacgcggcgg tgcaggaaga aatgaaagcg gaggtggacg cggtcctgaa
    10261 agcgggcgac acggtggggg ggatttttga aattgtggcg cacaatgtcc ccgtggggct
    10321 gggcagtcac gcgcaatggg acgagaagct ggatggacgg ctggcgcagg cgctgatgag
    10381 cgtgcaggcg gtgaaggcgg tggagatcgg cggaggcgtg gcgggagcgg gctccttcgg
    10441 aagcgaagtg caggatgaga ttttttatga aaagtcggcg agacggttcc ggcgtgcgac
    10501 gaatcgcgcc ggaggagtgg aaggcgggat tacgaacggc gaagacgtgg tcgtgcgagg
    10561 ctacttgaaa cccatttcga ctttgcgcca gccgctgcac actacggaca tggtgaccaa
    10621 agagcccgta aaggcggcgt acgagcggtc cgactggtgt gtggtgccgg caggcgcggt
    10681 ggcgggcgaa gccatggtcg cacttgtgct ggcggatgct tttctgcaaa aatttggggg
    10741 agactcgctc gcggagatgc gacggaattt cgacaactat gcccgacaaa tcgacgagtt
    10801 ctgaagcggc caacgccacg ggcaccgaga cggcgcaagc gctgtcggcg gcgcgcaaga
    10861 tctatccgat cgtcaagtac ggcgacgcca ttctggagaa gcccggcttg gcaatcagga
    10921 aattcgacgc ggaactggag cagctcgccg aggatatgtt cgcctcgatg tacgcggcgc
    10981 agggcgtggg actggcggcg ccgcaaatcg gaacgagcct gcggatcgca gtggtggacg
    11041 taaccggggg gaagaatccc gaagccaaga tcgtgctggc gaatccggag atcattcacg
    11101 cggaagggga agttcgggaa gaagagggct gcctctcggt tccgggcttt cgaggatacg
    11161 tgctgcggcc gcaatttgtg acgatcaagg cgcagaacgc caagggcgag acgtttgaaa
    11221 tacgcggcga aaacctgctg gcgcgcgcgt tctgccatga gatcgatcac ctgaacggaa
    11281 tcctattttt gcagcacctc agcatgctga agcgcgacct gatcaaacgg aaaatcaaga
    11341 aactcaggaa aaagggcgag tggtaagtgc cggagccggc cgcctagcgg cggcagtggg
    11401 ttaaagatgc gaatcgtttt ctgcggcacg ccctctttcg cggttcccac gctcaaatat
    11461 ctcctcgcac aatctgaatt tgaaattgtt tgggtgatta cgcagccgga ccggccgcgg
    11521 gggcgcgggc aggaagtttc tttttctccg gtgaaggaag cggcgctggc ggcgaatctt
    11581 ccggtgcatc agccggaaaa aattcgggcg cccgaaaccg aggcgctctt gcagaagctg
    11641 gcgcccgact gcgtcgtgat catcgcctac ggacaaatta tcccggcaag attgctgccg
    11701 attccgaaac tgggctggat caatttgcac gcttccctgc ttcccaaatg ccgaggcgcg
    11761 gcgcccatca actgggcgat cgtgaatggc gagacgcgaa ccggcgtgac gacgatgcgg
    11821 atcgatgcgg gcatggacac tggggaaatg ctgctgcaac gcgagatcga gattggcccg
    11881 aaagaaactg cacctgaact tgcggcgcgg atgtccgagt ccggcgcacc gcttatggcg
    11941 gagaccttgc gcgggttggc cgctggaact atctctccga aagctcagaa ccacgcggaa
    12001 gccagctacg cgccgatgct gaagaaagag gatgggcgga tcgattggaa gcagccggcg
    12061 atggagattt acaaccggat gcggggattc gcgccgtggc cgggggctta cacgacgttt
    12121 cgcgggcaga gttgccacgt gtggggcgag ccggtgtcta aagaaggagg cgctggcttg
    12181 ccgggcggag cggcgccggg aactttgttc ggcgaaaaaa atgaattgtt ggtgtggtgc
    12241 ggcgacgcaa ccgttttgtg cgttcggctc gtccaagtgg aggggcgcaa gccggtgaag
    12301 gcgagtgact ttgcgaacgg cgcgcgattg aaaagcgagg aacgctttgg cgacagttga
    12361 tttgcgcgag gcagaccact aggacatact tgaaattcaa gccttgcact tgaaagtaaa
    12421 cactctttga ctgaccgtgg catcgggagc tcaccttaca tgaagatctc agaatcctca
    12481 gcaaaacggg gaaaagtgcg tttccgcggg aaggaattgc tgcgtgccgc ggcttgctgg
    12541 gtgctcgttg cggagctagc gggattgcca ttggcgtcgg cggcgggcga caagtccgct
    12601 cccacaccag cgcccgcgag cgcggcgagc gatcccgtgc tgaaagcgat gcaaaacgag
    12661 atcacgcgag cgaccgcgga gctgggcaag acggagcagc cgccttacta ccttagttat
    12721 acggtgtacg accaggattt cgtcgttctg gtgggcgctt acgggagttt gctgaccaac
    12781 accacggcgc agcgacgctt cgcggatgtg aacatgcgag tggggctacc tgagctggac
    12841 aacacacatg gacagagccg tcccagcgga gtgaattcgg gcagcctgcc gctgggaaac
    12901 gacggggacg ccatcgcgcg cgtgttgtgg gaactgacgg atcgcgagta taagcgcgcg
    12961 gtgccgtcgc tgctgaacgt gcgaacgaac acggcggttc gcgcggaaga agaggataag
    13021 tcgccggact tctcgaagga gaagccacaa acgcacatcg gggaagcaga ggcggctgcg
    13081 ccattcgatc gcgccgcatg ggaaggcgag atcaaaagac tttccggagc attccggaag
    13141 tactcccagg tgtactttgc gacggtcatg atccaagtgc agcgttcgaa ttcacggatg
    13201 gtatcgagcg agggaagcgc catcgtgtcg ccgagcgctt ctacccggct gatcatggaa
    13261 gcgcagacgc gggccgagga cggcatggat ttactgcgcg tcgaaacgtt tcaggcgccg
    13321 acggcaagcg ggttgccgag cgaagcggaa ctgacgggca agatcgagaa aatggcggcg
    13381 gacttgagcg cgttgcgcaa agcgccggtg gcggagccgt acgatggacc ggcggtattg
    13441 agcgggcgcg cggcggccgt gttttttcat gaagtgctag ggcacagact agaaggccac
    13501 cggcagcgcg atgaagagga agggcagaca ttcacgaaga agatcgggca ggaagtgctg
    13561 ccgaaattcc tgagcgtgat cgacgatccg acaacgcacg agatggcagg aatgaagctg
    13621 gcgggcgcgt atgactttga caattaaggg gagccggcgc agcgcgtgga ggtgatcaag
    13681 gacggggtgc tggagaattt tctgatgtcg cggatgccga tcaagaattt cgggcaatcg
    13741 aacgggcatg gacgaaacca gcccggacgg atgcccacag gacgccaagg gaacttgatc
    13801 gtcacatcga caaaaacggt gcccgaagcc gaaatgcggc aaaagttgat tgatgaagtg
    13861 aagaagcaag ggaagccgta tggtttgtac ttcgacgata ttcaaggcgg gtttacgctg
    13921 acaacgcggg cgttgccgca ggcgttccag gtactgccgg tgcttgtgta caaggtttat
    13981 gccgacgggc gtcctgatga attggtgcgc ggggtggaca tcgtgggtac gccacttgcg
    14041 gcgttgacgc ggattctgac aaccggagat cagccgcgcg tatttaacgg tgtttgtggc
    14101 gcggagagcg ggcaggtgcc ggtcgcagca gtggcaccgt caatgctttt ctccgagatg
    14161 gaagtgcaga agcgtgcgca cgagcatgaa cgtccgccgc tgctgccccc accgggattt
    14221 gaagatgccg gcgccgccaa agatgcgaag gcgaaggcga aggtgaagcc atgaagatac
    14281 ttcaccggcc gaaggcattg cgtgtttaca aacaatgggc aggaatagtg attgcggctt
    14341 tgctggcgat gaacgccacg ccaaaccggg ccgcaaccga gggggcgaaa gcggcaccgg
    14401 acgcggctat ggttgcggcg cacgcggcgg cgaagggcga tggtctgctc gaggcgctgc
    14461 tcacggaatt ggaccggtcg aaagcgcagc tgaaaatgga tcaggtgcag gcgccgtatt
    14521 acgtcgagta tcgcgtgaac gaagtcgaag atattggagc ggaggccgct tacggggcct
    14581 tgcgcgagag ccagcacatt catgttcgcg tcctgcgggt ggtggtgcgc atcggcgatt
    14641 acaggcaaga cagctatttc ggcaggggcc agggcgaatc cagcattttg cctctggaca
    14701 acgatccgat tgcgctgcgg caccagatct ggctggcaac ggatgaggct tataaagctg
    14761 cgggccaggc actggcggag aaacaggcgg ccatgaagca gttcagcgtg gacccgaatc
    14821 ccgtggacga ctttgcgaag gcgccgcaaa tgattgccgt ggagccgacg gcgtcgctga
    14881 aaatcgacga agcggggtgg aagaaaacgc tggaagatgt gacgagtttg tacaagcagt
    14941 atcccgacgt gcagtcggtg acggcgtcgg cgaggttttc ggcgatcaac gaatacctgg
    15001 tgaattccga aggcacggtc acgcgcagcg gaaagacgac gtacagcgtg caattgagcg
    15061 gcacagcaca agcggccgac ggaatgcggc tgagccggag cccagcgttc atggtggcgc
    15121 ggccggaaga attgccgacg cacgacgcgc tgatgggcga agcgaagaag atgctggaaa
    15181 cggtggtggc actgcgacaa gctccgatcg tcgaggagga atatcgcggg ccggtgttgt
    15241 tcgcggcgga tgcggcggac gacatcgtgg cgtcgttgat cgggcagaac gtgttggggc
    15301 aaaagccgca acttgggaaa ccaaaccgga cgacgggagc atttgcgacg agctacaagg
    15361 cgcgcgtgct gccaaatttt ctgtcggtgg tggacgatcc gacgatgaaa gatttccagg
    15421 gcaagagcct ggtgggaagt tacgcggtgg acagcgaagg cgtgaaagcc caagcggtca
    15481 acaccatcga aagcgggatg atggcgaatt atttgatcgg acgagtgccg attcgcgatt
    15541 tcgccacctc gaatggccac gggcgggccg gggcgggatc ttttcccggg ccgagcctcg
    15601 gtgtgctgct ggtgaaaagc tccgaggcgc aagcgcctga agagctgaag aaaaaaatca
    15661 tccagatggt gacggagcag ggcaaggctt acggctatcg tgtggagacg ctcgggccag
    15721 gcaattcgcc gcggctgctc tatcgcgtgt atgcgaaaga tgggcatgaa gaattggtgc
    15781 gaggcgcggt gttcagcgaa ctggatattc gggcgctgag gaatgatttg atcgccgtgg
    15841 gcaacgatcc gctggtgagc aatagagccg gcggtgcgcc gacgacgatt atcagcccgt
    15901 cgctgctttt tggcgaactc gaagtcaaac gcgccgacac tagcaaggac aagctgccgg
    15961 attatccggc gccgcctttg aagaagtgat tagttttcag tctttagttc ttagttttta
    16021 gttcttagca gaatgtggcg ggctgaatgc cgcccctgcg catacagtct tatgcggggc
    16081 aagtcttacc gacgccggcc tgaaggccgc cgctacgggc ggtgctggct cagcatggtt
    16141 ggaaagaagt gcgcggaata ggaacttcgc cggattaccc ttaagagaac gtctttttgt
    16201 acccccccac tcccccctgt ttttcataag tgttcattct aaagatagtt aaagtccttt
    16261 gttttgatac acttttacaa gtgttgattc taaagggact tacgttgcac caaaattgtg
    16321 caagattgga tgatttcgcc ttttttttcg tgagcactca ttctaaaggc ttttaagttc
    16381 cttacaatga acactttagt agcctctgta agtgttgatt ctaaagggac ttacgttgca
    16441 ccaaaattgt gcaactgcct tgtctgctag ctaagtgggg atggatactg gaccggggct
    16501 acgggattaa cgacagatgt acctattatt taggatacca gagaagcggc agtctgtcaa
    16561 gagaaaagaa cagcgggtag tgtcctagtt ttggtgcgcc tttcgtcggc ctttcttggc
    16621 gggggcgatg agaggagcgg tcagtttttc gtagagggcg aagaggtgtt cgacgcgctg
    16681 gcggtcgttc tggaaaggct gcgggcggta acagaggtca acggcacgat ccaagtcggc
    16741 atgggttttg acgagtacag gcggcatcgc caacgggtca tagagatcgg ccaacgtcgc
    16801 ttccggaaat actttgcgcg catccaggac cgcttgcgcg gccgcttgga cggcctcgcg
    16861 ctgtttggcc ttcggctgtt cgggccatgg atagttgttg tagacgaggt tgcttgagta
    16921 ccggtatcgc gactcaagac gcccacatac caggcgcacc cacgccatgt gcatcaccga
    16981 ggagagaatc ccgaagtggt agggtgtggc gttagaaatt atgaggcagc tgtcgcccgc
    17041 cagaatttct ggtttgacat acccgatggg gatatatttt cgtcgttcgg agctgtggcg
    17101 aggaat
//
LOCUS       DASYVO010000195        13829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_74121, whole genome shotgun sequence.
ACCESSION   DASYVO010000195 DASYVO010000000
VERSION     DASYVO010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 13829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13829
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74121"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1553)
                     /locus_tag="VGR03_06590"
     CDS             complement(45..1553)
                     /locus_tag="VGR03_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993977.1"
                     /translation="MYGFVPCGRSIQPAPAHDATPVCLMHSRDPQKSDATFQKEFEAI
                     LEAARNGFADFTRFVFPRASYAERKFTAACVFSLATFTRYASFSGATFTQDANFFRAT
                     FTRDAIFFGATFTQKTRFLRAKFLGSAEFRRTHFRLDQELFPGPIFSLAEFSQLEAVI
                     FYKTYLGQALFHNCDVSRVNFSSVEWRKRKGSGKSMVFEEEVDLEAAKDLKPGKDSPD
                     ERDFGLIAELYQQLKKNYDERKDYWTAGDFHCGEMEVKRLARPRAERLARLSDWLKSR
                     ARHTAWVDGTEKLVNRGRRWWHRRLSLMAWYKRASQYGESYARPALWLVTILFAFALL
                     FPVAGLRYDPAKDPHAVSGAAQSSYVLTYWHPFPPGQASTSRHKAQWRLFGNSCVTAL
                     DVATFQRDRTTSPLIHGADCCSCWSYCSSRRCLGFFCWRCSDNSGGRGMNAKRREISH
                     TRRSDGAAVSLRRQRKRDFSLPQADAFAGAKAEEISRPALFEMTGWRGRRQE"
     gene            complement(1825..1983)
                     /locus_tag="VGR03_06595"
     CDS             complement(1825..1983)
                     /locus_tag="VGR03_06595"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEV1993978.1"
                     /translation="MYPISTVRDIKRPVTIRVDADVLAWLKRQRRGYQTRINKLLGEA
                     MEGKRRGA"
     gene            2047..2472
                     /locus_tag="VGR03_06600"
     CDS             2047..2472
                     /locus_tag="VGR03_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993979.1"
                     /translation="MNYGRIALAAVASTVVYYLYGFLVEGLLIRKDFSPYSAVYRSAD
                     TVMGYIPLGFACTLIGTFVIAMIYAKGYEGGSGAAEGARFGLLVGIFVVCTFVGPNYV
                     TLNIGRKLALELAASNFVQWIIVCVVIGLIYKPASAAGQ"
     gene            3178..3567
                     /locus_tag="VGR03_06605"
     CDS             3178..3567
                     /locus_tag="VGR03_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993980.1"
                     /translation="MAKTKQFTIAIDNQPGAVAAIARTLGDAKVNILALLGTAQGTAG
                     SVQLVVDDARRAKKALDAARIAYQETPAETYDLPNKPGALAQCLDKLAAKGVNLNSIH
                     ATAAKGGKKAVVVYTAEAAAKAAIATA"
     gene            3723..4877
                     /locus_tag="VGR03_06610"
     CDS             3723..4877
                     /locus_tag="VGR03_06610"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HEV1993981.1"
                     /translation="MTLLLFFGFLFCVLPARALENGLALTPPMGWNSWNRFGCNVSED
                     LVKSAADALVSSGMKDAGYEYVVIDDCWQVSRDAAGNIVVDVKNFPNGIKPVADYVHS
                     KGLKFGIYSDAGTHTCQMRPGGRGHEYQDALQYAAWGVDYLKYDWCNTGTQDAPASYA
                     TMRDALKTAGRPIVFSLCEWGTSKPWLWAKDVGNLWRTTGDISDCWNCKKDQLSWTQI
                     LDLQVGLETYAGPGHWNDPDMLEVGNGGMTTTEYRSHFSLWCMLAAPLMAGNDLKNMS
                     PEIKEILTNREVIAVNQDLLGRQGRRVRKNGDSEVWAKQMKDSSRAVVLLNRGAADAE
                     ISISWEDLGYPAHLSAAVRDLWAKKDLGKSTATFNAKVPSHAVVMLRVTP"
     gene            5464..5889
                     /locus_tag="VGR03_06615"
     CDS             5464..5889
                     /locus_tag="VGR03_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV1993982.1"
                     /translation="MNESNEVLRILVVDDSTVSRNLAEYVLAGKPYKVFFADGAQQAL
                     KLFASHRPDVVITDWMTPELSGLELCRMIRNKLKCRDTYLILLTSNSRNDHIAEGLAA
                     GADGYLTKPLRSEELLGQLRTARGVLKTRRQMETNKSLS"
     gene            complement(6015..7091)
                     /locus_tag="VGR03_06620"
     CDS             complement(6015..7091)
                     /locus_tag="VGR03_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993983.1"
                     /translation="MKRTLTICEAILLAAVFTSAQEKPAAAPNEMEHMQHGGFMQEGM
                     HHAVAKGFTLDAKVDGLAHTVTLRVGPMNLPAHTSHIKMPQPPDLVFEVPMGGWLIAY
                     HPKLVDAGGNAVPGTVLHHVALWNENRADFLCPNKEEHIFGAGSEMTDWAEIPGFGYR
                     VEKGNKIRIETMMYNPTATSYERVYLEVVIPFQDTQEAASAAAPRKSVYPAWMDVASC
                     GNSGYDLPAGKSEKSGTVKVKYDGVLLGVGGHLHDYGEQIVLQDVSRKETVATLDAKV
                     DAQGHLESVPVKLFVQEGGYKFAAGDVLEISATYYNPTGKLLRDGAMGIAVGYFVATD
                     DAKMAALRRNAAPPHDISGMSHDH"
     gene            complement(7229..8164)
                     /locus_tag="VGR03_06625"
     CDS             complement(7229..8164)
                     /locus_tag="VGR03_06625"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEV1993984.1"
                     /translation="MKKVALFSLLLVVLLSATHSNAQEKTANNPAGAPREPMLVTVDW
                     LADHLSDPSVVLLQIGDKKDYDKGHIPGAQFLEYESISTPHGQGLMLELPPVVQLVSV
                     FERLGVSNRSHVVLYFGTNWVTPTTRVYWTLDYLGLGDRTSILNGGLVAWQATHHPVS
                     TEMKQPAKGSIAPAPRNEIVADAEWVSSHLNKPSVTIIDARTHEFYNGSQSDGNPRSG
                     HIPGAANLSYLDVLDQDNNKFKSPDALKELFRAAGLKPGNLMVSYCHIGQRATVLYFT
                     AKMLGYEAKMYDGSWEDWSHRKDLPIVTGDAPGKP"
     gene            8303..9733
                     /locus_tag="VGR03_06630"
     CDS             8303..9733
                     /locus_tag="VGR03_06630"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV1993985.1"
                     /translation="MKIHERFAIHFGILFLLVLLFSEPIHAQKPAADDLLVLNNVTVV
                     DVRTGALQPEQTVILERNHIASVGPSKSAKVPRNAPSVNCRALFLIPGLWDMHVHLVF
                     GDWFPGARDISLPLFVANGVTGVRDMGSELDIVQGWRNEIEAGRLIGPRIYTSGPMLD
                     GPKPRFPSSVAITTPEDAHRAVADLKRRGADFIKLQSLIPRDAVFAIAEEAKKQEIPF
                     QGHVPDAVRASEMSAAGMKSFEHLIGIFEGSSPAEDEFLKGNKTKGRFLATYDPARAA
                     SLASILAKNQTWQGPTLVWERGGNLIDASDFSKDERVKYVPGSWKNKTWKRFTDEITQ
                     GYGTDDLAIRKRFVEKELEVVGMLHKAGVPFLAGTDTPAGVHIFPGYSLHEELQRFVA
                     AGFTPLEALQTATINPARFFAMEDQAGTVEKGKFADLVLLSANPVEDIANTQKIAAVI
                     VNGQYYRRAELDKMLGRVETAAHRQP"
     gene            9851..10705
                     /locus_tag="VGR03_06635"
     CDS             9851..10705
                     /locus_tag="VGR03_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV1993986.1"
                     /translation="MKLIRIGEVGREKPGVLLEDGARLDVSGFGADYNEAFFAGDGIA
                     KLRNWVATHGGSAPRASSAVRLGPPICRPSKIVCIGLNFRDHAEESGMEIPKEPVIFF
                     KATSAVAGPNDAVVIPKNATKVDWEVELAVVMEKKESYVSRENALDYVAGFVLHNDYS
                     ERSFQLERGGQWVKGKSADSFAPLGPFLATKEEIQDFNQLGMWLNVNGEFRQRGSTSK
                     MIFDVPALVAYVSEFMTLLPGDVISTGTPPGVGLGMKPPQYLKAGDVVELGIDGLGES
                     RQEVVDYP"
     gene            10900..11754
                     /locus_tag="VGR03_06640"
     CDS             10900..11754
                     /locus_tag="VGR03_06640"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV1993987.1"
                     /translation="MQIDAHQHFWRYSQREYGWIDDSMAALRRDFLPADLKPELERSG
                     FQGCVAVQARQTLDETRWLLELAERNPFILGVVGWVDLRSSGVGFELEALAYESKLVG
                     VRHVVQSEPDDRFVLQPEFLCGISMLEEFDLAYDILIYPRHLPVAAEFVERFPRQRFV
                     LDHLAKPPIKSGALEPWARGIRELASFPNVYCKVSGMVTEADWQAWQPADMRPYLDVA
                     FECFGPSRLMIGSDWPVCTVAGSYSRVMDLVKGYLSKYSAEERDAVLGGNAVKFWRLK
                     LQENQARD"
     gene            11844..13493
                     /locus_tag="VGR03_06645"
     CDS             11844..13493
                     /locus_tag="VGR03_06645"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEV1993988.1"
                     /translation="MQNKVLKLDSKDNVLISLADLRRGEQISFDSQTYTLESDVPAKH
                     KFATENLDNGASIIMYGVLVGKAVEPIRRGGLLTTRNIHHQAATFQEKTEDYRWAPPD
                     VSRWSQRDFPGYRRSDGQVGTRNYWIVVPLVFCENRNIAVLKQAFEEELGFAAPQVYR
                     RQVAELARLYADGRSEEIKTRALTGVAEGRQRSRVFENVDGVRFLLHEGGCGGTREDS
                     NNLCGLIAGYIHHPNVAGATVLSLGCQHAQIGILREELRKRNSNFDKPLVIVEQQQSG
                     TEVAMLTEAIRKTFLGLMEANRCRRTREPLSSLCVGLKCGGSDGFSGLSANPAIGHTA
                     DILAALGGRTILSEFPELCGVEQELINRSARKEVAGRFMQLMKSYGARAIAVGSGFEM
                     NPSPGNMRDGLLTDAMKSAGAAKKGGNSPVKAVLDYPEYSTEPGLNLQCTPGNDVECV
                     TAQVGAGANVVLFTTGLGTPTGNPIAPVVKLATNSGLAQRMRDIIDVDTGEIISGEKT
                     IEQMGEEILEFVIRTASGETRTKAEGKGQEDFIPWKRGISL"
     gene            13469..>13829
                     /locus_tag="VGR03_06650"
     CDS             13469..>13829
                     /locus_tag="VGR03_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1993989.1"
                     /translation="MEKRNLTLKSPGSGAKDFRLDGKVAIVTGGGSGIGQAIALRFAA
                     NGATVRIADINLQQAVATAKQISEAGGQGTAHACDVTNQQQVREVFNGLFAKERVEIL
                     VNNAGISHVGTVESTTEE"
BASE COUNT         2939 a         3858 c         4093 g         2939 t
ORIGIN      
        1 ccatcttcca ctccgcccgc cgctggcccg ataccttcta gccgttattc ttgccgccgc
       61 ccccgccacc ccgtcatttc gaacagagca ggccgactta tttcttccgc tttcgctcct
      121 gcaaaagcgt cggcctgcgg aagtgagaaa tctctcttcc tctgcctccg cagagagact
      181 gctgcgccat cactccgccg tgtgtgagaa atctctcttc tcttcgcgtt catcccccta
      241 ccgccggaat tgtcgctgca ccgccagcaa aaaaagccca aacaacgtcg ggatgagcag
      301 taactccagc aggaacagca gtcggccccg tggataagcg ggctcgtagt acggtctctt
      361 tgaaaagtgg cgacatccag cgccgtaacg cagctgttcc caaataatcg ccattgggct
      421 ttgtgccggc tcgtgcttgc ttgtcctggc gggaaagggt gccaatacgt cagaacgtaa
      481 gaagactgag ccgccccgct caccgcatgc gggtctttgg ctgggtcgta acgcagacct
      541 gccacaggaa acaacaaagc gaaagcaaac aagatcgtga ccagccagag cgccggccgc
      601 gcataacttt ctccatactg gctcgcgcgt ttgtaccacg ccatcaggct cagccggcgg
      661 tgccaccagc gccggccgcg atttaccagc ttctcggtcc catcgaccca agctgtgtgc
      721 cgtgcccggg atttcagcca gtcggagagc cgcgcaagcc gctccgcgcg cggtctggcc
      781 aggcgcttca cttccatttc gccacaatga aagtcgcccg ccgtccaata gtctttccgc
      841 tcgtcgtagt tctttttcaa ttgctgatag agttcggcaa tcaagccgaa atcgcgctcg
      901 tccgggctgt ccttccccgg cttcaggtcc ttggcagctt caagatcgac ttcttcctcg
      961 aacaccatgc tcttgccgct gccttttcgc ttgcgccact ccaccgacga aaagttcact
     1021 cgggacacgt cgcagttgtg gaagagtgcc tgacccaggt aagtcttata gaagatgacc
     1081 gcttccagtt gggagaactc cgccaaggaa aagatagggc ccggaaaaag ctcctggtct
     1141 aatcggaagt gcgttctgcg gaactccgcc gacccaagga actttgctcg cagaaaccga
     1201 gtcttctgcg tgaacgtggc accgaagaag atggcgtccc gcgtgaacgt ggccctgaag
     1261 aaattggcgt cctgcgtgaa cgtggcaccg ctgaagctag cgtaccgcgt gaacgtggca
     1321 agagaaaata cacatgccgc cgtgaatttt cgctctgcgt agctcgcgcg gggaaagaca
     1381 aacctagtaa aatctgcaaa cccatttcgg gcagcctcca ggatggcctc gaactctttt
     1441 tggaatgtcg catcgctctt ttggggatcg cgggaatgca tcaggcagac gggcgttgca
     1501 tcgtgggccg gcgcaggttg aatcgagcgg ccgcagggca caaaaccgta catctcaacc
     1561 gggcagcccc cggatgatga atctctctcc gaggccgctt ggtcctccgg agcagccgta
     1621 gcacggccat ctcgttgctc tttttcttcg gactcggaca cgttgggccg agattttagc
     1681 agaagttatc gctccgtacg attgccgggt gccggttttg ggctggtggc aggcccttag
     1741 tcgctgggca agacgtgggc gctgcatgcc ccgaccgggt cggggcatgc gggttttgat
     1801 tcttcctgaa caatcttgcg gcgcctacgc tccccgccgc ttcccttcca tggcttcacc
     1861 caggagcttg ttgatccttg tctggtaacc acgtctctgg cgcttcagcc acgccagcac
     1921 atcggcatcc acgcgaatcg tcaccggtct cttgatatca cgaacagtcg agatgggata
     1981 caatcgctgc cgtttgcgag gtcaaacagg gaattccaaa taaattggcc taacgaaagg
     2041 gtggtcatga actacggacg aatcgctctc gccgctgttg cctccacggt ggtctactac
     2101 ctctatggct tcctcgtgga aggcctgctc atcagaaagg acttttctcc ctactccgcc
     2161 gtataccggt cggctgatac ggtgatgggc tacataccgc tcggattcgc ttgcaccctc
     2221 atcggtacct tcgtgatcgc catgatctac gcgaagggct acgaaggcgg ctccggcgcc
     2281 gcagaaggcg cccgctttgg tctgctggtc ggaatcttcg tggtgtgtac gttcgtaggg
     2341 cccaactatg tgaccctcaa catcggcagg aaactcgcac tcgagcttgc ggcaagtaat
     2401 ttcgttcagt ggatcattgt atgcgtcgtg atcggactaa tctacaaacc tgcatcggct
     2461 gccggacagt gatctgccgg ccctgttact cctggttcct actctccgat ctctgattcc
     2521 ttattttcaa ccttctaacc tcgcaacctg catacttttc aatatcttag cgcctcattc
     2581 gaatgatact caataaatac attgacatat atactagttt atgatattct tgattgtgtt
     2641 tgtttatatt gagcctcgaa ctgtaaatcc ccgatccacc ttccccctgc ggctcgcgcc
     2701 ctgctgcgtt gctcgcttgt cttcctatat ctcatttgtt tccttaacct cctctaagtc
     2761 ctttcccttc acgtcccttc acgctttttg cctacccccg ccccttaacc tcaatcacat
     2821 ctatctttta caaaaacata cgggggaggg gggtgccgcg ttccttggac gctcggacgt
     2881 tgagacctgc agatatttca aggtgttccc gagctatccc ctttcttttg atattcttgc
     2941 gcactctttt gcgcacacca aaagcacaac tcatttattt tcaggcgatt ccgcactctt
     3001 tgccaagaac acccgggagt ggggtggggg ccccaagctt caaaccttgc aacccgcaaa
     3061 ctttcaatcg tccccgtggc catctgttcc ttttcgtccg ttccttacct ccttacttca
     3121 ttacctctta tttcccatcg gcgtataatc ccagccagca cggcaggagg tgccgacatg
     3181 gccaagacca agcaattcac aatcgctatt gataatcagc ccggtgcggt cgccgcaatc
     3241 gcgagaaccc ttggggacgc caaggtgaat attctcgctc tcttgggtac cgcccaaggc
     3301 accgccggat cggttcagct cgtagtcgac gatgcccggc gcgcgaagaa ggccctcgac
     3361 gcggcccgca tcgcttacca ggaaactccc gcagaaacct acgatttgcc caacaagccc
     3421 ggtgcgctgg cgcaatgttt ggacaagctc gcggcgaaag gcgtaaacct gaattcgatc
     3481 cacgccaccg ccgccaaagg cggtaaaaag gccgtcgttg tctacactgc ggaagccgct
     3541 gcaaaagctg caatcgccac cgcgtaggag catctctctc cttccgtggt ccttccgttc
     3601 atcaggcgcg gagtgtccgc tccaggcgag cgtgggtagt tttgtcgttg ggccctgaaa
     3661 gaaatgaaac aagaataagc cgaaaggttt tcatggatgc ctttatgaag aaaacatgtt
     3721 gtatgacgct gttactcttc ttcgggtttc ttttttgcgt tttgcccgcg cgcgcccttg
     3781 aaaacggttt ggctctcact ccccccatgg gctggaacag ttggaaccgt ttcggctgca
     3841 acgtcagcga ggatctcgtc aagtctgccg ccgacgccct ggtcagcagc ggcatgaagg
     3901 acgccggcta cgaatacgtc gttatcgacg actgctggca agtcagccgc gacgctgccg
     3961 gcaacatcgt cgtcgacgtg aagaattttc ccaacggcat caaacccgtc gccgactacg
     4021 tccattccaa gggcctgaaa ttcggcatct actctgatgc cggtacgcat acctgccaga
     4081 tgcgccccgg tggccgcggc cacgaatatc aggacgccct gcaatacgcc gcatggggcg
     4141 tcgactatct caaatacgat tggtgcaaca ctggtacgca agacgctccc gcctcctatg
     4201 ccaccatgcg cgacgcgttg aaaaccgctg gccgtccgat tgttttcagc ctctgcgaat
     4261 ggggtaccag caagccctgg ctctgggcca aggacgtcgg caacctctgg cgcaccaccg
     4321 gcgacatctc cgattgctgg aattgcaaga aagaccaatt aagctggacg caaattctgg
     4381 atttgcaggt cggcttggaa acttacgctg gcccgggcca ctggaatgat ccggacatgc
     4441 tcgaagtcgg caatggtggc atgaccacca ccgaataccg ctcgcacttc agcctctggt
     4501 gcatgctcgc tgcgccgttg atggccggaa acgatttgaa gaatatgtct ccggaaatca
     4561 aggaaatcct caccaatagg gaagtcatcg ccgtcaatca ggatctcctc ggcaggcagg
     4621 gccgccgcgt gcgcaaaaac ggcgactccg aagtctgggc caaacagatg aaagacagca
     4681 gccgcgccgt cgttttgctg aatcgtggtg ccgccgacgc cgaaatctcc atctcctggg
     4741 aagacctcgg ctatcccgct catctctccg ccgccgtccg cgacctgtgg gcaaaaaaag
     4801 acctcggcaa atccaccgcc actttcaacg cgaaagtccc ttcccacgcc gtggtcatgc
     4861 tccgcgtcac gccctagaat ttcgccggtc tccagaccag cgctatggct cagtccgtag
     4921 tcttttgtgt tgcgcggtgc agcgcaaagg gctacgataa atcccgcgtg ggagaaaggc
     4981 cctcaaatgg gcacgaatcc cacgacccgc cacaatttag ttgatttgac ctgcccgcgc
     5041 tgccaccaga aaaattcggt tgcggtcgat ctgcgttctg gagccagtaa ctccggcatg
     5101 cggaaagttc atttcgccta ctgcggaaag ccgtggaata cgcacccttc agggccggtc
     5161 atgcccgagc ccttcccgag atgaatacgc ttttgagagg acgcgcgtaa aggactacct
     5221 caagaggccg gaagacgtca gcagggcagt gcggctgctg aggcatgacg gagtgcgcgt
     5281 tggactgccg atgcaaaaag ccgatggcga gatgatcttc ccggtggggg acttcacgct
     5341 atcagctggg gagatcctcg agttgctcga caataacgaa ctgcaccacg caggaatcca
     5401 ccggcttggg gaggcacaga aagggaacgc gccagacact ccggcgcagt ccaggagcaa
     5461 agtatgaatg agtccaacga agtcttgcgc atcctggtag tggatgattc gacagtttcg
     5521 cgcaatcttg ccgaatacgt tctcgccgga aaaccctaca aagtcttctt cgcggatggc
     5581 gcgcagcaag ccctcaaact atttgcaagc catcgtccgg atgttgtcat caccgactgg
     5641 atgacgcccg agctgtccgg attggaactt tgccggatga ttcgcaacaa actgaagtgc
     5701 cgggacacct atcttattct gctgacgagc aattcgcgca acgaccatat cgcggaaggt
     5761 cttgccgccg gagccgatgg ctacctcacg aaaccactcc gctcggaaga gctgctcggc
     5821 cagcttcgta ccgcgcgagg cgtcctcaaa acgcgccgcc agatggaaac gaacaagagc
     5881 ctctcttgaa ggctgatgcc gcgcggatct ttctccgcat ccgctccgaa cgcctttagc
     5941 tcactccttc aggagtgagg tctttctgtt gtcttgcact ggacaccggc cactgaccac
     6001 aaatcacttt cttctcagtg gtcgtgggac atgcccgata tgtcgtgagg aggcgcggcg
     6061 ttgcgacgca gagcggccat cttcgcatcg tcagtcgcga caaaatagcc aacggcgatg
     6121 cccatcgctc cgtcgcggag cagcttcccc gttggattgt agtacgttgc ggaaatttcc
     6181 aggacatcgc ccgccgcaaa cttgtaaccg ccctcctgga caaacagctt cacgggcacg
     6241 gattcaagat gtccttgagc gtccaccttc gcatcgagtg tcgccacggt ctctttgcgc
     6301 gacacatcct gcagcacaat ctgttctccg tagtcgtgta aatgtccgcc caccccgagc
     6361 aacacaccgt cgtatttcac tttcaccgtt ccgctcttct cgcttttccc cgccggcagg
     6421 tcataaccgg aattcccgca cgacgccaca tccatccacg cgggataaac actctttcgc
     6481 ggcgcggcgg cgctcgcggc ctcctgcgtg tcctggaagg gaatcacgac ttcgagatag
     6541 actctttcgt aggacgtggc cgtggggttg tacatcatcg tctcgatgcg aatcttgttg
     6601 cctttctcca cgcgatatcc aaagccgggt atttcggccc aatccgtcat ctcactgccc
     6661 gcgccgaaaa tatgttcttc cttgttgggg cagagaaaat ccgcgcgatt ctcgttccat
     6721 aacgccacat gatgcagaac cgttcccggc acggcgttgc cgccggcatc cacgagcttc
     6781 ggatggtacg cgatcagcca gccacccatc ggcacttcga aaaccaggtc cggcggctgc
     6841 ggcattttta tgtgcgaggt gtgcgccggc aaattcatcg ggccaacgcg gagcgtaacg
     6901 gtatgcgcca gtccatccac tttcgcatcc agcgtgaagc ctttggcgac cgcatggtgc
     6961 atgccttcct gcatgaagcc gccatgctgc atatgctcca tttcattggg cgctgccgcc
     7021 ggtttttcct gggccgaagt gaagaccgcc gccagcagaa ttgcttcgca aattgtgagc
     7081 gttcttttca ttcgatttct ccgcgcgcgg atcccaccgt acatccggaa acttcccaca
     7141 tcatactccc gcgaagggag cagaggaatc gcgcagcaat gcgcgttgcg cgaagtgagc
     7201 aatggaaacg cgaaaacggc aacgctggtt acggcttgcc gggtgcatcg ccggtgacga
     7261 tgggtaagtc tttgcggtgg ctccagtctt cccaggaacc gtcgtacatt ttcgcttcgt
     7321 agcccagcat ttttgcagtg aagtagagaa ccgtggcgcg ctgtccgatg tggcagtaac
     7381 tcaccatcaa atttcctggc ttcagccctg ccgcgcgaaa taattccttg agtgcgtccg
     7441 gactcttaaa cttgttgttg tcctgatcga gcacgtcgag ataggaaaga tttgccgcgc
     7501 ccggaatgtg cccgctgcgc ggattcccgt cgctctgcga tccgttgtag aactcgtggg
     7561 tgcgcgcgtc gatgatggtg acggaaggct tgttgaggtg gctactcacc cactccgcat
     7621 ccgccacgat ttcgttgcgc ggcgccggcg cgattgaacc tttcgccggc tgcttcattt
     7681 cggtggacac ggggtggtgg gtggcctgcc acgccaccag tcccccattc aaaatcgaag
     7741 tgcggtcgcc caggcccagg taatcgaggg tccagtaaac gcgggtggtg ggcgtcaccc
     7801 aattcgttcc gaaatacaaa acgacgtgcg agcgattcga aacgccgagc cgttcgaata
     7861 cggagacgag ttgtacgacg ggtgggagct ccagcatcag gccttggccg tgcggcgtcg
     7921 aaatgctttc gtactcgagg aactgcgcgc cgggtatgtg gcccttgtcg taatcttttt
     7981 tgtcgccgat ctggagcagc acgacggaag ggtcgctgag gtggtcggcg agccaatcga
     8041 cggtgaccag catgggttcg cgcggcgcgc cagcagggtt gttcgcggtt ttttcctgag
     8101 cgttcgaatg ggttgccgag agcagaacaa cgagaagcaa agagaaaagc gctacttttt
     8161 tcatacggtg tttttgaggg gccagtgccc cttatgacgt actacgctat agcgcatgac
     8221 gccatagtgt caagcgctga tgcaagtagg acgaagttct ttcaggtgaa ctgcggcggg
     8281 aaagcagata gtatgcggag cgatgaaaat acacgagcgt ttcgccatcc acttcggcat
     8341 tctttttttg cttgtcttac ttttctccga gcccatccac gcacagaagc ctgcggctga
     8401 cgatctgctg gtgctcaaca atgtgacggt ggtcgacgtt cgaacggggg ctttgcagcc
     8461 ggagcaaacc gtaattctcg agcgcaatca cattgcttcg gtggggccta gcaagtcggc
     8521 gaaggttccg cggaacgcgc cgagcgtgaa ctgccgcgcc cttttcttga ttcccggact
     8581 ctgggacatg cacgtacatc tggttttcgg cgactggttt cccggcgcgc gggacatttc
     8641 gctgccgctc ttcgttgcga acggcgtcac cggtgtgcgg gacatgggca gcgagctgga
     8701 catcgtgcag gggtggcgga atgaaattga agcggggcgg ctgatcgggc cgcgcattta
     8761 cacctcaggg cccatgctgg atggaccgaa gccgcgcttt ccgagttcgg tggcgattac
     8821 gacgcccgaa gatgcgcatc gcgccgtcgc tgatctcaag cggcggggcg cggacttcat
     8881 caagctgcaa tcgctgattc cgcgcgatgc ggtttttgcc attgcggaag aagccaaaaa
     8941 gcaggagata ccattccaag ggcatgtacc ggacgcggtg cgcgcgagcg agatgtccgc
     9001 agcggggatg aaaagtttcg agcatctcat cggcattttt gaagggagtt cgcccgccga
     9061 ggacgagttc ttgaaaggga acaagacgaa agggcgattc ctggccacgt acgatcccgc
     9121 gcgcgccgct tcactggctt cgatccttgc gaagaatcag acatggcaag gcccgacgct
     9181 ggtgtgggaa cgcggcggca acctgatcga cgcgagcgat ttcagcaagg acgaacgcgt
     9241 gaaatatgtg ccgggatcgt ggaaaaacaa aacgtggaag cgattcaccg acgaaatcac
     9301 gcagggctac ggaacggacg atctggcgat tcgaaagaga tttgtcgaga aggaactgga
     9361 ggtcgtgggg atgttgcaca aggccggggt gccgtttctt gcgggaacgg atacgcccgc
     9421 tggcgtccat attttcccgg gctacagcct gcacgaggag ttgcaacgtt tcgtggcggc
     9481 gggattcact cctctcgaag cgctgcaaac cgcgacaatc aatcccgcgc gttttttcgc
     9541 aatggaagat caggccggaa cagtcgaaaa aggaaaattc gccgacctgg tgctgctttc
     9601 cgccaaccct gtcgaagaca ttgccaacac gcagaagatt gccgccgtca tcgtgaacgg
     9661 ccagtactac cgccgcgccg agctcgacaa gatgctcggc cgcgtggaaa ccgccgcgca
     9721 tcgtcaacct tgaacggtgg aacgcgctct gggaatgaag acccgcgatc gagcgagtgc
     9781 actcccggta gttggctcct tttttctgcg ggcgttggta aacttttcac tacattggag
     9841 cgtttctgaa atgaaactga ttcgaattgg cgaggtgggg cgggaaaagc cgggcgtgtt
     9901 gctcgaggat ggagcgcggc tggatgtttc cgggtttggc gcggactata acgaggcgtt
     9961 tttcgcgggt gacggaattg ccaaactgcg gaattgggtg gcgacgcatg gtggttcggc
    10021 gccgcgcgca tcatctgcgg tgcgtttggg gccccccatt tgccggccca gcaagatcgt
    10081 gtgcatcggg ctgaattttc gggaccacgc cgaagagagc gggatggaga ttcccaagga
    10141 accggtgatt tttttcaagg cgacatcggc ggtggcgggg ccgaatgatg ctgtggtgat
    10201 tccaaaaaac gccacgaaag tggactggga agtggaattg gcggtcgtga tggagaagaa
    10261 agagagttac gtcagcaggg agaacgcatt ggattatgta gcgggttttg tgctgcacaa
    10321 tgattattcg gagcgcagct ttcagcttga acgaggaggg cagtgggtga agggaaaaag
    10381 cgcggattcg ttcgcgccgc tggggccgtt cctggccacg aaagaggaaa ttcaagattt
    10441 caatcagctg ggcatgtggt tgaatgtgaa tggagagttc cggcaaagag ggtctacttc
    10501 caaaatgatt ttcgatgttc cggcgctggt cgcttacgtc agcgaattca tgacgcttct
    10561 gccgggagac gtgatcagta cagggacgcc accgggcgtt ggcttgggga tgaagccgcc
    10621 gcaatatctg aaggcgggcg atgtcgtgga gctgggaatc gacggtttgg gggaatcgcg
    10681 gcaggaagtc gtggactacc cgtgatagaa gattcgagtc cgacagaaac gtcaaagcaa
    10741 gtcttagagc aggctgctga aaaaggcagc tcggagcaac tggcggcgat cagatgagtt
    10801 tcgaccgcat tgagaacact gctcttacaa aaagcgcaca gccaggattg gctgtgccac
    10861 tgggtttttt cggcggcttg ttaaatcagg gcgcgcgcga tgcagattga cgctcatcag
    10921 cacttttgga ggtatagcca gcgggagtat ggctggatcg atgactcgat ggcggcgctg
    10981 cgccgggatt ttcttccggc cgatctgaaa cccgaactgg agcgcagcgg gtttcaggga
    11041 tgcgttgcgg ttcaagcgcg gcagacgctg gatgagacgc gatggctctt ggaattggcg
    11101 gagcgaaacc ccttcattct cggagtagta ggctgggtgg atctgcgatc gtcgggagtg
    11161 ggattcgaac tggaagcatt agcgtacgag tcaaagctcg tgggcgtccg ccacgttgtg
    11221 cagagcgagc cggatgaccg gttcgtgctg caaccggaat ttctttgcgg catctcgatg
    11281 ctggaagaat tcgatctggc ctacgacatc ctgatttatc cacggcatct tccggtggcg
    11341 gcggagttcg tggagcgatt tccgcggcag cgctttgtgc tggaccatct ggcgaaacca
    11401 ccgatcaaga gtggagccct tgagccgtgg gcgcgcggaa tccgggagct ggcgtcattt
    11461 ccaaatgtat attgcaaggt gtctggaatg gttacggaag cggactggca ggcttggcag
    11521 cctgcggaca tgcggcctta tctggatgtg gcgttcgaat gctttgggcc gagccggttg
    11581 atgattggat cggattggcc ggtgtgcacg gtggcgggtt cgtattcgcg ggtgatggat
    11641 ttggttaagg gctacctcag caagtactcg gcggaagaaa gagatgccgt gctgggagga
    11701 aatgcggtga agttttggag attgaagctt caagagaatc aagcgcgcga ctaatcgcgc
    11761 ggcgtgttct acaatgcggg ggaaaagcgg ggcagtggct gttgtaaacg cttgcgaaat
    11821 gggaaatcac gggtctgggc gaaatgcaga acaaggtact caaactcgat tcgaaagata
    11881 acgtgctgat ctcgctggcc gacctgcggc gaggggagca gatttccttt gattcgcaga
    11941 cttacacact agaatcggac gttccggcaa aacacaagtt cgctacggaa aatctggaca
    12001 atggcgcgag catcatcatg tacggcgtgc tcgtcgggaa agcggtggag cccatccggc
    12061 gagggggact gttgaccaca cgcaacatcc accatcaggc cgcaacgttt caggagaaaa
    12121 ccgaagatta ccgctgggcg ccgccggacg tatcacgatg gagtcagcgg gactttccgg
    12181 gatatcgccg atccgacggc caggtgggca cgcgaaatta ctggatcgtc gtgccgctgg
    12241 tgttttgcga gaaccggaac attgcggtgc tgaagcaagc gtttgaagag gagctgggtt
    12301 tcgccgcgcc acaagtctat cgacggcagg tggcggaact ggcgcggctc tatgcggatg
    12361 ggcggagcga agagatcaag acgcgcgcgt tgacgggagt tgcggagggc cggcagcgtt
    12421 cccgagtttt cgaaaacgtg gacggcgtgc ggtttctgct gcatgaaggg ggctgcggag
    12481 gaacgcggga ggattcgaac aatctctgcg gactgatcgc gggttacatc catcatccga
    12541 atgtggccgg cgccacggtg ctcagcctgg gctgccagca tgcgcagatt ggaattctgc
    12601 gcgaggaact gcgcaagcgg aattcgaatt tcgataagcc actggtgatc gtggaacagc
    12661 agcaaagcgg cacggaagtt gcgatgctga cggaagccat ccggaagacg tttttgggtt
    12721 tgatggaagc aaatcggtgc aggcggacgc gcgaaccgct ttcgagtttg tgcgtcggat
    12781 tgaaatgcgg tggctcggat ggtttttccg gcctctcggc gaacccagcc atcgggcaca
    12841 cggcggatat tctggcggca ctcggcggga gaacgatact ttccgaattt ccggaattgt
    12901 gcggcgtgga acaggaactc atcaatcgca gcgcgcgcaa ggaagttgcg gggcggttca
    12961 tgcaactgat gaaaagttac ggggcgcggg cgatagcggt cggatcggga tttgaaatga
    13021 atcccagccc agggaacatg cgcgacggat tattgacgga cgcgatgaaa tcggcgggcg
    13081 cggcgaaaaa gggcggaaat tctccggtga aggcggtgct ggactatccg gagtattcga
    13141 ccgagccggg gctgaacctg caatgtacgc cgggaaacga cgtggaatgc gtaacggcgc
    13201 aagtcggcgc cggagcaaac gtggtgctgt ttacaaccgg cctggggaca ccgaccggaa
    13261 atcccatcgc gccggtagtg aaactagcga cgaattcggg gctggcgcag cggatgcggg
    13321 acatcatcga cgtcgacacg ggcgagatta tttctggaga gaagacgatt gagcagatgg
    13381 gcgaagagat tttggaattt gtgattcgaa ccgctagcgg agagactcgc acgaaggccg
    13441 aagggaaagg ccaggaagat tttattccgt ggaaaagagg aatctcactc tgaaaagccc
    13501 gggcagcggc gcgaaggatt tcagacttga cggcaaagtt gccatcgtga ccggcggcgg
    13561 cagcggcatc gggcaggcga tcgccttacg cttcgccgcg aatggggcca cggttcgaat
    13621 tgccgatatc aatctacagc aggctgtagc taccgcgaaa cagatcagcg aagcgggcgg
    13681 gcaaggaacg gcgcacgcgt gcgacgttac gaaccagcag caagtccggg aagttttcaa
    13741 cgggctcttt gcgaaagagc gtgtcgagat cctggttaac aacgcgggga tatcgcacgt
    13801 gggcacggtg gagagcacga cggaagagg
//
LOCUS       DASYVO010000196         8407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_105677, whole genome shotgun sequence.
ACCESSION   DASYVO010000196 DASYVO010000000
VERSION     DASYVO010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105677"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..905
                     /locus_tag="VGR03_06655"
     CDS             162..905
                     /locus_tag="VGR03_06655"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050825 - ice binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HEV1993990.1"
                     /translation="MKKVLLVICMLSVGGLAWAQSAPPLASAQSFAVLGASTVTNTGP
                     TIITGDLGVSPGTAVTGFPPGTVTGGTIHAGDATAATAQAAAHTAYGNLVAQPCGTNL
                     TGKTLGTSPGAVTLSPGVYCFDSSAQLTGTLTLNGRGVYVFKIGSTLTTASDSSVVLA
                     NGATTGKVFWQVTSSATLGTNSAFEGSILALISDTVTTGTSVTGRVFALTGAVTLSGN
                     RIRAASKHDRKDNDRESKNHKDNDHKDHE"
     gene            complement(1539..1880)
                     /locus_tag="VGR03_06660"
     CDS             complement(1539..1880)
                     /locus_tag="VGR03_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1993991.1"
                     /translation="MKREATAVEQVYRNFVVLGVNNHCVRLAVMQGEFRWHQHPRSDE
                     CFLVLDGQLEIDLADGRTFRLNPGEAFTIPAGLIHRTRAHARAVNLCFEDRNAYTDVI
                     FEDPARGPEAK"
     gene            complement(2025..2807)
                     /locus_tag="VGR03_06665"
     CDS             complement(2025..2807)
                     /locus_tag="VGR03_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993992.1"
                     /translation="MPIESELVAHLTEGGYHPRTSKHSDFQSLIIIRDLVAQCPLLGR
                     HATSGEIVAKLRHHQQVGHADWVIDIAIGTCAGAPKPPLEVDLSESPIKMAPPVIIRI
                     AVELKSIFTEHGKARLNRLRDFNSFHGYAHQYDQKTVAAAFLVVNASERFYSPLREEN
                     DITVHRTSKKTARQVADSAVKIFRSIHLRSSPSDAPGMEAVGVVVVEHDNLAFHPDRA
                     TYGGLHRSTRIAPTPPNLPLGDPLHYESMIQRICNAYTQRFG"
     gene            complement(2815..3720)
                     /locus_tag="VGR03_06670"
     CDS             complement(2815..3720)
                     /locus_tag="VGR03_06670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV1993993.1"
                     /translation="MAKPSTALPKSIARTRVTRHLVRECDARDLSFIETESTHLVCTS
                     PPYGSLKTYPDHPSQLGNIASYERFLAELDVVWADCLRILVPGGRIACVVGDICISRR
                     QAGRHYVLPLSADLQVRVRKLGFDCLTPIRWQKVANIRLEASRSSRFLGKPNLPNGIV
                     KNDMEHILFFRKPGGYRKPSDEMERRSFIPTSEYARWFCPIWTDVTGQIRKEHPAPYP
                     LEIPRRLIQMFSFVGDTVVDPFGGTGTTALAALLLGRNSITVEIEPRYVDLIAHRLQS
                     ERGASVIDIDRPKHQPVRQNLAAIG"
     gene            complement(4193..4582)
                     /locus_tag="VGR03_06675"
     CDS             complement(4193..4582)
                     /locus_tag="VGR03_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993994.1"
                     /translation="MALTRDFRETVQADAKRDPAFRRGLLSDALESLLSGEVALGKEL
                     LRDYINATVGFPRLAAQTKIHAKTLHQMFGPHGNPTASNLFAIVAYLQDAEGVRFELR
                     SPRPALRTKKRPSPRKTRRAAPAAMSR"
     gene            complement(4696..6015)
                     /locus_tag="VGR03_06680"
     CDS             complement(4696..6015)
                     /locus_tag="VGR03_06680"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV1993995.1"
                     /translation="MHGKVAWRTVLGLLILGGLAAPALMWSQTLPPLQTVAVRAGRLF
                     DPRSGTNLTNQVVLINGDRITDVGPADRVQIPAGARVIDLSHATVLPGLIDGHVHLTD
                     AAGGLQHQMMVGLYSATQSLRAGFTTLVSMGTHGGGYADVELRKAIESGLVQGPRLLT
                     AGPIIQITTPGSATYPLDWKPFEVNLVANGPEAMRAAVRELAHYGADHVKIYTTGPFS
                     FKPNGEMVVPALFSLEELKAVVDEAHRRGMWVASHTYGGEGLKWALEAGVDDIQHAIA
                     ADDADIQMFVKKNLPLTATILDQRQDEPGDLKRFAPYSRWRLMEQTWKKMLAAGVRLG
                     FGSGAAPPPLRVYDLACYCSHGVQAEMFPIFVKWGATPAYTLRMATTVNAEIIHMQDS
                     LGAIEKGKFADLIAVSGDPLQDITEMQRVKFVMKGGEVVRNDLPPAR"
     gene            5997..6842
                     /locus_tag="VGR03_06685"
     CDS             5997..6842
                     /locus_tag="VGR03_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993996.1"
                     /translation="MPLSHALPFLLESDSDLGQKATRICRWHAIARRSPAERQGLHRV
                     IPYNSLVGNRAYLSVWCREFSEATLPFTFQSFLDTVPVSKSRAGFTQLTIRAVSHGEP
                     PAIEHDLRGQPLNAAEIVAAASEILHDDVCYEVEAHWDLWSYDVKQKAWRLGPQRLEI
                     LCQGEQYDDGACEQSGHFCVDLGFEDLFTGEGNLIGRADAPALMPEQSPDGEEHPTQK
                     ELLQFLAQPGRRREVQEKTRSNILGLQDWMGRILSELPVVGVRLWSEGEENFEDRVDA
                     ILAAE"
     gene            6805..7440
                     /locus_tag="VGR03_06690"
     CDS             6805..7440
                     /locus_tag="VGR03_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993997.1"
                     /translation="MKIVLMQFWQRSRRPGFRRGVAALAVASLLCAASLAAAPAQKAG
                     QKIAQKAEYRANELTLSGLRPGRDTRANALARYHKPPGASDEKTAAWRDLCRGRELRM
                     EFGENDVVESITAAVIAGPHYDCHPGTPLMAAETWRTGRGLSLGDMRERVVALYGEPE
                     SRGPSAREGEETEFLYYPFDWAGSDVPQVLEVTCDMKSGRVVQITLARQSL"
     gene            7440..8069
                     /locus_tag="VGR03_06695"
     CDS             7440..8069
                     /locus_tag="VGR03_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993998.1"
                     /translation="MRAHSKKRMSLGLALGLLSLACAACWYAPANNDKSGAAAAPAPA
                     TPEEETAAAKAALGSDAEVLVFGPLGGEGSRQVVAINRIPGAAWTAAATGTPVTGTAV
                     SRVSILSKENGSWREVLRCDEHLKNPSGYLHGTPAMGVAGWHLEIEQQTDRGRVLHFT
                     PLAPPAAVKSGAIAVAWNPKAKRYQSLDRSGREFQGESSALEIGQSRLK"
     gene            8066..>8407
                     /locus_tag="VGR03_06700"
     CDS             8066..>8407
                     /locus_tag="VGR03_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1993999.1"
                     /translation="MSAMAQTVNVSKPRESSWRNLQKLVPYLLHYKARMAMGLATLVT
                     GGMVGALLPLAVGTMIDSLSGSRSVLDRLEALPGPLRVLLVSFYRPFNRETLLFYGLV
                     LVGIVTLKGVLS"
BASE COUNT         1708 a         2423 c         2623 g         1653 t
ORIGIN      
        1 cagctgccat ctgtttgttt cgcgggtgtt caaaagtgga cagatgcggg tttgcaccag
       61 ctgttagcat ccgggtcggt tgggaaactc gaagtcgact cgacaatgcc ctgccccatt
      121 gtcagcgacg atgaggacaa atccagccaa ggagcttcga tatgaaaaaa gttttgctgg
      181 ttatttgcat gttgagcgtg ggcggactgg cctgggcgca gtcggcgcca ccacttgctt
      241 ccgcgcagag cttcgcagtt ctgggagcct caacggtgac gaatacgggc ccaacgatca
      301 tcaccggaga tttgggtgtg agcccgggta cagctgtcac cggctttcct ccggggaccg
      361 tgaccggagg aacaatccac gcgggcgatg cgaccgccgc cacggctcag gccgctgccc
      421 acactgcata cggtaatcta gtcgcgcaac cctgcggcac gaacttgacc gggaagactc
      481 taggcaccag tcccggcgcg gtcacgctct cgcctggcgt ctattgtttt gattcttcgg
      541 cgcagttgac cggaaccctc acgctcaacg gcaggggtgt ctacgttttc aaaataggga
      601 gcacgcttac gacagcgagc gattcctctg ttgtcctggc caacggagcg acgacaggaa
      661 aagtgttctg gcaggtcacc agctctgcca ctcttggcac gaacagcgct tttgaaggaa
      721 gcattctcgc gctcatcagc gacaccgtca ccaccggcac cagtgttacc ggtcgagtct
      781 ttgcgctgac gggtgctgtg acgctgagcg ggaaccgaat tcgcgccgca tcaaaacacg
      841 accggaagga caacgaccgg gaaagcaaaa accataagga caacgaccat aaggaccacg
      901 agtaaactga gaaatgttat tcggaggcgg cgggtgaccc aatctcaggt cggaccaact
      961 gtcttgagtt gtgggtgcgg gaggcccacg tgccgagttt tcacgtgggg atttctcttc
     1021 cactcatcgc cagttgaacc gccccacgga gaaggtttat cctgattcca cttcggtgac
     1081 gaatatccac gacaacaacg gaaacctggc cacccgggtg ggcgggtggc ccggaggcgg
     1141 attttcttaa ccgagcggta cggcgggagc atgcctcacc tacaatctgc aacaatggat
     1201 ccctgcgacg gctccaggca gttcccgcca gcggctgaag gaagcaagag gaggaaaacg
     1261 tacccgcaga ggataggaaa cagagctcga gcagttccca caagagcgtc ggaaccctct
     1321 aatgctctca aaactcaatt tggaaaggtt tattcacggc ttcggggacg gaaaggatgc
     1381 ggcacccggc gggggagcag tgacgagtga tcccgaagtt tcgggagtga cgggtggcga
     1441 gtgacgagcg gtgaagatct aggtctggcg aaaaaccagt tcaactggcc cacaatcctc
     1501 aggccacaga cgtaagaccg acgcatcgaa caatctcttc acttggcctc cgggccacgc
     1561 gccgggtctt cgaaaatcac atcggtgtag gcgttgcgat cctcaaaaca cagattcacc
     1621 gctctggcat gcgcgcgagt tcgatggatc aagcctgcgg ggatggtaaa cgcctctccg
     1681 ggattcaaac gaaaggtccg tccgtcggca agatctatct ccaactggcc gtccagaacg
     1741 aggaaacact catccgagcg gggatgctga tgccagcgga actctccctg catcaccgcc
     1801 agccgaacgc aatggttgtt tacgccgaga actacgaaat ttctgtagac ctgttcgacg
     1861 gcggtggcct cgcgtttcat gtctacaaca gttagagctg gaaacgcggc ttgctcagtg
     1921 tgggtggcaa catcctcgtt ttgctgagtt ctgtaatctg tcatgcgcct tagattcacg
     1981 agggccggtc atggttacga atggctgcgc cacccgcccc tcccctatcc aaaccgctga
     2041 gtataagcat tacagatccg ctgaatcatc gattcgtaat gtaaagggtc tccaagcggc
     2101 aaatttggcg gggtcggagc gatgcgtgtt gaccggtgca gcccaccgta agtggctcgg
     2161 tctggatgga aggccaagtt gtcgtgctcg acaaccacaa ccccaacagc ctccattcct
     2221 ggcgcgtccg aaggagagct acggagatga atgcttcgga agattttgac cgcgctgtca
     2281 gccacttgcc gcgctgtttt ctttgaggtc ctatgaaccg taatgtcatt ctcctcacgc
     2341 agtggcgaat agaaccgttc ggaggcattg acgactaaga acgctgcagc cacggtcttc
     2401 tggtcgtact ggtgcgcgta cccgtgaaac gagttgaagt cgcgcaaacg gttcagtcgt
     2461 gccttgccgt gttcggtgaa gatcgatttc agctctaccg caattcggat gataaccggc
     2521 ggtgccattt tgatgggact ttcgctgaga tctacttcta gaggtggttt cggcgcgcct
     2581 gcgcatgttc caatcgcgat gtcgataacc caatcagcgt ggccgacctg ctggtgatgc
     2641 cgaagcttcg cgacaatctc gccgctcgtt gcatgtcggc cgagaagcgg gcattgagca
     2701 accaagtcac gaatgatgat gagagattgg aagtcgctgt gctttgacgt gcgcgggtgg
     2761 taaccgccct cggtcagatg ggccaccagt tcagattcga tcggcaccgt cgccctatcc
     2821 gatggccgct aggttctgcc gaacaggctg gtgtttaggt ctatcaatgt cgatgacact
     2881 agctccacgc tcggactgaa gccgatgcgc tatgaggtcc acgtagcgag gctcgatttc
     2941 aactgtaatg ctgttccggc ccagtaacag ggccgccagc gccgtggtac ccgtccctcc
     3001 gaacggatcg acaacggtgt cgccgacgaa actgaacatc tgaataagac gacgtggaat
     3061 ctcgagcgga taaggtgcgg gatgttcttt gcgaatctgg cccgttacat cggtccagat
     3121 gggacaaaac catcgcgcat actcactggt cgggatgaaa gatcgccgct ccatttcatc
     3181 gcttggcttg cggtagccac caggcttgcg gaaaaacaaa atatgctcca tatcattctt
     3241 gacgataccg ttaggcaaat tgggcttgcc gaggaatcga ctagacctcg aagcttcaag
     3301 tctaatgttg gcgaccttct gccaacggat cggcgtgagg cagtcaaacc caagctttcg
     3361 aaccctaact tgcagatccg ctgaaagcgg taacacatag tggcggccgg cctgccggcg
     3421 agagatgcat atatcgccga ctacgcatgc tatccgacct cccggcacca ggatacggag
     3481 gcagtccgcc caaacaacat caagttctgc cagaaatcgc tcgtacgacg cgatgttgcc
     3541 gagctgggag ggatggtcag ggtaggtttt gagcgaccca tatggagggc tggtgcaaac
     3601 tagatgagtg ctttcggttt cgatgaagga tagatcacgc gcgtcacatt cgcgcactag
     3661 atgtcgcgtt acacgggtcc tagcgatgct tttcggtaat gcagtgctcg gcttagccat
     3721 gttgcttctc ccctgagcca ttagccttga cagtcgccaa attttgacgc cgcgtcgagt
     3781 caactcattg aatgcctgaa tcatcgtata ttgtgtcctc gccgttgaca agcgcatcaa
     3841 actggaccgt ttcgcatgtc gattaagatt agaacttgaa actactctag atggcatgct
     3901 cagccgcaag gctgggccgc cacttatgag cgcgaagggt acccggcttt agtcgcagtt
     3961 caatgcaccc acagttttgg acaacctgag gctccgaaac ttgtcataga tgcgaatgtt
     4021 gggagcgcca cgcgaggcgt tcgattccgc aggctctggt gcggccgttg gtgcttggtc
     4081 tctgcggagc taccggcagg cggccatgct aggccgtggc gcttgcgcga gacaggcgca
     4141 cagccatccc gaagtttcgg gagtggctat gctaccggcc taactggctc gattaacgac
     4201 tcatcgcggc gggggcagcg cgtcgtgttt tccgaggaga agggcgcttc tttgtgcgta
     4261 aggcggggcg aggggagcgg agctcgaagc gcacaccttc ggcgtcctgc aagtaagcaa
     4321 cgatcgcaaa caggttgctg gctgtgggat tcccgtgggg gccaaacatc tggtgaagag
     4381 tcttggcgtg aatcttagtc tgcgcggcca gcctgggaaa gccgacggtg gcattgatat
     4441 agtcgcgcaa cagctctttg ccgagcgcga cctcgccgga gagaaggctt tccagcgcat
     4501 cgctcagaag ccctctgcga aaggcgggat cacgtttggc atccgcttga accgtttctc
     4561 tgaaatctcg cgtcaaagcc attttcattc ctcttgatgg tagcatatat tgtaccacgg
     4621 tcaagcgcga aatcttcccg ctaatgagct tcgtgtgctg ccggaatgac cggaatgaca
     4681 ctgcggaagc ggagatcatc gcgccggggg cagatcgttg cgcaccacct ctccgccctt
     4741 catcacaaat ttaacgcgct gcatctcggt gatgtcctgc agcgggtctc cggagacggc
     4801 gatgaggtcg gcgaacttgc ccttttcgat ggcgcccagg ctgtcctgca tgtggatgat
     4861 ctcggcgttg accgtggtgg ccatgcgcag cgtgtacgcc ggcgtcgcgc cccatttgac
     4921 gaagatggga aacatctccg cctgcacgcc atgcgaacag tagcaggcca agtcgtacac
     4981 ccggagcggc ggcggagcgg cgccgctgcc gaaaccgagc cggacaccgg cggcaagcat
     5041 tttcttccaa gtctgctcca tgaggcgcca gcggctgtag ggcgcaaagc gtttcaagtc
     5101 acccggctca tcctgccgct gatctaaaat tgtcgcggtg aggggaagat ttttcttaac
     5161 gaacatctgt atgtcggcat cgtcggcggc gatggcatgc tggatatcgt cgacgcccgc
     5221 ttccagggcc cacttcagac cctcgccccc gtaggtatgg ctggcaaccc acatgccccg
     5281 ccggtgcgct tcatccacga cggccttgag ttcctccagg ctgaacagcg ccgggactac
     5341 catctcccca ttcggcttga aggagaacgg gccggtggtg taaatcttta cgtggtcggc
     5401 gccgtaatgg gccaactcgc gcacggctgc gcgcatggcc tccggtccat tggcgaccag
     5461 gttgacttcg aaaggtttcc agtccaacgg gtaggtggcg cttcctggag tggtgatctg
     5521 gatgatcggc ccggcggtca gcaggcgtgg accctggacg aggccgctct cgatggcttt
     5581 gcgcaactcc acgtcggcgt acccgccgcc gtgcgttccc atgctcacca gcgtggtgaa
     5641 gccggcgcgc aaactctgcg tggcgctata cagccccacc atcatctggt gttgcagtcc
     5701 cccggccgcg tccgtgaggt gaacgtggcc gtcaatcaga ccggggagca ctgttgcgtg
     5761 gctgaggtcg atcactcggg cgccagcggg aatctgcacg cggtcggccg gaccaacgtc
     5821 ggtgatccgg tcgccattga tgagcaccac ctgattggtc aggtttgtgc ccgacctggg
     5881 gtcgaacaac cggccggcgc gaaccgctac ggtctgaaga gggggaagag tctgggacca
     5941 catcagcgcc ggagccgcca gtcccccgag aatcagaagt ccaagaaccg ttctccatgc
     6001 cactttccca tgcattgcca ttcctcctcg agagcgacag cgacctggga cagaaggcta
     6061 cacgaatctg ccggtggcac gcaattgcgc ggcggagtcc cgcggagcgc caaggcctgc
     6121 atcgcgttat cccttataat agcctggtgg ggaatcgcgc ttatctgagc gtgtggtgcc
     6181 gggagttttc cgaagccacg ctgccgttta cgtttcagag ttttctggac acggtaccag
     6241 tgtcgaaaag ccgcgcgggg ttcacgcagc tcaccattcg cgcggtgagc cacggggagc
     6301 cgccggccat cgagcacgac ctgcgcgggc aaccgctcaa cgcggcggag attgtcgctg
     6361 ccgccagcga aatccttcac gacgatgtct gctacgaagt ggaagcgcac tgggacctgt
     6421 ggagctacga cgtgaagcaa aaggcctggc gactggggcc gcagcgcctg gagattctct
     6481 gtcagggcga gcagtacgac gacggcgcat gcgagcaaag cgggcacttc tgcgtggacc
     6541 tgggcttcga agacctgttc accggggaag gcaacctcat cggccgcgca gatgccccgg
     6601 cgctgatgcc ggagcaatcg ccggacggcg aagagcatcc aacgcaaaaa gagttgctgc
     6661 agttcctggc gcagcccggc cggcggcgcg aagttcagga aaagacgcgg tccaacatcc
     6721 tgggtctgca ggattggatg gggcgaatcc tgtcggagct gccggtggtt ggcgttcggc
     6781 tgtggtccga aggcgaggag aactttgaag atcgtgttga tgcaattctg gcagcggagt
     6841 aggcggccgg gcttccgccg gggagttgct gcgctcgctg ttgcttccct gctttgcgcg
     6901 gcctcgcttg ccgcagcccc ggcacagaaa gccgggcaaa aaattgcgca gaaggcggaa
     6961 taccgtgcga acgaattgac gctttccgga ctgcgcccgg ggcgcgacac acgcgcgaat
     7021 gccctggccc gttaccacaa gccgccgggc gcgagcgacg agaagacggc cgcgtggcgc
     7081 gacctgtgcc gcggccgcga attgcgcatg gagttcggcg agaacgacgt ggtggaaagc
     7141 atcaccgccg cggtgattgc ggggccgcat tacgactgcc atccgggaac gccgttgatg
     7201 gcggcggaga catggcgcac cggccgcggc ctgtcgctcg gcgacatgcg cgagcgggtc
     7261 gtggcacttt acggcgagcc ggaatcgcgc ggcccttccg cccgggaggg cgaggagacg
     7321 gagttcctgt actatccatt cgattgggcg ggatcggatg tgccgcaggt gctggaagtc
     7381 acctgcgaca tgaagagcgg gagggtggta cagattacgc tcgcccggca gagtttgtga
     7441 tgagagcgca ttcgaaaaaa cggatgagtc tcgggctggc gctcggtctt ctctctctgg
     7501 cgtgcgcagc gtgctggtat gcgcccgcaa acaatgacaa atccggcgct gcggccgcgc
     7561 ctgcgcctgc aacgcccgag gaggaaacag cggcggccaa ggccgcgctg ggcagcgacg
     7621 cagaggtttt ggtgtttggc ccgctgggcg gggaaggctc gaggcaagtg gtggccatca
     7681 accggatccc cggcgctgcc tggacggctg ctgcaactgg gacgcctgta accggaacag
     7741 cggtgtcgcg cgtctcaatc ctgagcaaag aaaacggatc gtggcgcgaa gtgctgcgct
     7801 gcgatgagca cctgaagaat ccgagcgggt atctgcacgg gacgccggcc atgggtgtgg
     7861 cgggctggca cctggagatt gagcagcaga cggaccgagg ccgcgtgctt cacttcactc
     7921 ctctcgcacc gcccgcggcc gtgaagagcg gcgcgatcgc cgtggcctgg aaccccaagg
     7981 cgaagcgcta ccaatcgctc gaccgcagcg ggcgggaatt ccagggggaa agttcggcgc
     8041 ttgaaattgg gcagtcgcga ctgaaatgag tgccatggcc caaaccgtga acgtcagcaa
     8101 gccccgcgaa tcgtcgtgga gaaatctcca gaagctcgtc ccctatctcc tgcactacaa
     8161 ggcgcggatg gccatggggc tggcgacgct ggtgaccggc ggaatggtgg gggcgctgct
     8221 gccgctggcc gtcggaacga tgatcgactc gctttcgggc tcgcggtcgg tgctcgatcg
     8281 cctcgaagca ttgcccggcc ctcttcgcgt tctcctggtt tccttctacc gccccttcaa
     8341 ccgcgagaca ctgctgttct acgggctggt gctggtgggg attgtgacgc tgaagggcgt
     8401 gctctcc
//
LOCUS       DASYVO010000197         2460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_48976, whole genome shotgun sequence.
ACCESSION   DASYVO010000197 DASYVO010000000
VERSION     DASYVO010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48976"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VGR03_06705"
     CDS             complement(<1..675)
                     /locus_tag="VGR03_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1994000.1"
                     /translation="MKKDRSARIRIVSLAPSATSILCAIGAKGALVGVTRWCADVAPV
                     KGLPELGDCWKLDVEEVLRLKPTLVIGSVPYKPETVAEILTNSLTFLAMNPRSLADVE
                     SDIVLLGRLTGRNGAARKAVARMRKEFAAIAKKARRAKRPPRVYCEAWPNPRISSPPW
                     VAELVRMAGGEMVLPPGQRVTEEQVAEACPEVIILAWAAAGDKSKPEKTYSVEAWKDL
                     PAIRNKR"
     gene            827..1861
                     /locus_tag="VGR03_06710"
     CDS             827..1861
                     /locus_tag="VGR03_06710"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEV1994001.1"
                     /translation="MHKSIFMAALAIGSALGAAGAAAQQQSPKTVHYEATLENVKYTF
                     GVAPPVAHLRPGDTLDANSLDCFGNAIKKPGDTLALVHGDNPLTGPFFIEGAEPGDTL
                     VVQILDLQVDGKQGIGAFAPGFGALNETNYTPMLHPPLPERIWFYPIDKDSNTATFQA
                     LDSKFTTKIPLHPFLGCIGVAPAGGEARSSVVPAEFGGNMDAPEASAGNTLYLPVNVS
                     GALFYFGDGHAAQGDGEVAGTAIEVPMRVRLRFGLLKGKKLNWPRFENDRAIMAAGIY
                     RPLDDALRIAFTELVGWIHADYGLSELDAYELLSKVAKIHLTEMVDPNYVVIASIEKK
                     FLPPRKTSQR"
     gene            complement(1958..2434)
                     /locus_tag="VGR03_06715"
     CDS             complement(1958..2434)
                     /locus_tag="VGR03_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994002.1"
                     /translation="METIISISDPEKGIHYSLFPRMKVGFRMQEPVAFLKEMESRRTT
                     PWAPQWDQNFTRQILGNQMIEGLQCEGRLDTVTIPAGSTGNSFPIEIVTERWYSADLQ
                     INLIVKHTDPRSGETTIRLTNVSLEEPQKALFEIPPGYEIREGGTTTIPLAEKKKP"
BASE COUNT          462 a          787 c          688 g          523 t
ORIGIN      
        1 tcgcttgttt cgaatcgcgg gtaaatcctt ccacgcctcg actgaataag tcttctccgg
       61 ctttgatttg tcacccgccg ccgcccacgc gaggatgatc acctccggac aggcctcagc
      121 tacttgctcc tcagtcaccc gctgacccgg cggcaggacc atctcgccgc ccgccattcg
      181 aactaactcg gccacccagg gcggggaact gatccgcgga ttcggccacg cctcgcaata
      241 cacgcgcggc ggccgctttg cgcgtcgcgc cttctttgca atcgcggcga actctttccg
      301 catccgcgcg acggccttgc gcgcagcgcc gttgcgtccc gttaatcgtc cgagcagaac
      361 aatatctgac tcaacgtcgg ccagcgagcg cggattcatc gccaaaaacg taagactgtt
      421 cgtaaggatt tcggcgaccg tttcaggttt gtatgggacc gatccaatga cgagcgtggg
      481 cttaagtcgc agcacttcct caacgtcgag cttccagcaa tcgcccaact cgggcaggcc
      541 tttcactggc gccacgtccg cgcaccacct cgttacgccg accagcgcac ccttcgcgcc
      601 gatggcgcac agaatggaag tcgcgctcgg cgccagcgag acaatcctga tccttgcgct
      661 ccggtctttc ttcatgggcc tccaggcgat agtaatcgcc ccgggagatg gctgcaatcc
      721 gcaatgcacc ctggtccggg aatttcatct cgcccggatt gactcctcgc acgcggtatg
      781 atgcactctc gtctcttcac tcacatttgc gagggaggag tacttcgtgc acaaatcaat
      841 cttcatggcg gcgcttgcga tcgggtcggc tcttggcgcg gccggtgccg cggctcagca
      901 gcagtctccg aagaccgtgc actacgaggc cactctcgaa aacgtgaaat acactttcgg
      961 cgtcgcgcca ccggtcgcac accttcgccc cggtgacacg ctggacgcga actccctcga
     1021 ctgtttcgga aacgccatca agaaaccggg cgacacgctt gcgctcgtcc acggtgacaa
     1081 tccgcttacg ggccccttct tcatcgaagg ggcggagccc ggcgacacgc tcgtggtgca
     1141 gattctggat cttcaggtgg acggcaagca gggcatcggc gctttcgctc ccggtttcgg
     1201 cgcgctcaac gagaccaatt acacgccgat gctccatccg ccgctgccgg agcgcatctg
     1261 gttctatccg attgacaagg attcgaacac cgcgacgttc caggcgctcg attccaaatt
     1321 caccacgaag attccgttgc atccttttct cggctgcatc ggcgtagcgc ccgccggcgg
     1381 agaggcgcgc agctccgtgg tccccgccga gttcggcggc aacatggacg ctcccgaagc
     1441 ctccgcgggc aacacgctct atctgccggt gaacgtttcc ggcgcgctgt tctatttcgg
     1501 cgacggccac gcggcgcagg gcgacggcga agtcgccggc acggccatcg aggtgcccat
     1561 gcgtgtccgc ctgcggttcg gtttgctcaa aggcaagaag ctcaactggc cgcgctttga
     1621 aaacgaccgc gccatcatgg ccgcgggcat ctatcgcccg ctcgacgacg ctctgcgcat
     1681 cgccttcacc gaactggtcg gctggatcca tgcggactac ggcctctccg agctcgatgc
     1741 ctacgagctg ctgtcaaaag tggcgaagat tcacttgacg gagatggtgg acccgaacta
     1801 cgtggtcatc gcttccatcg agaagaaatt cctcccgccg cgcaaaacct ctcagcggtg
     1861 atttgaagta tgactttgag gcacccggcc tcaccgccac ggcatcgaag agcggcgcgg
     1921 atgaagatgc ggcgctgctc aaaaaggaaa tccctaacta cggctttttc ttctcggcga
     1981 gaggtattgt agtggttccg ccctcgcgaa tctcgtagcc gggaggaatt tcaaacaagg
     2041 ctttctgggg ttcctcgagg ctcacatttg tcaggcggat cgttgtttcg ccgctgcggg
     2101 gatcggtgtg tttgacaatc aagtttatct ggagatcggc cgagtaccat cgttcggtaa
     2161 cgatttctat ggggaaagag ttgccggtgg atccggccgg aattgtcacc gtgtcaagtc
     2221 tcccctcgca ttgtaagcct tcgatcatct ggtttccgag gatctgcctt gtgaagttct
     2281 ggtcccactg cggagcccaa ggcgtcgttc ggcgagactc catctccttc agaaaagcga
     2341 ccggctcttg catccgaaat cctactttca tcctcggaaa aagggagtaa tgaattcctt
     2401 tctcagggtc actgatcgaa atgatggtct ccatgagagt ctgttgatcc tgtttcccgg
//
LOCUS       DASYVO010000198         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_59758, whole genome shotgun sequence.
ACCESSION   DASYVO010000198 DASYVO010000000
VERSION     DASYVO010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59758"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..329
                     /locus_tag="VGR03_06720"
     tRNA            256..329
                     /locus_tag="VGR03_06720"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:288..290,aa:Gly,seq:ccc)
     gene            complement(750..983)
                     /locus_tag="VGR03_06725"
     CDS             complement(750..983)
                     /locus_tag="VGR03_06725"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEV1994003.1"
                     /translation="MEKLERITTDPARMNGEPCIRDLRLTVRRVLEALAIYPDRAALK
                     CEYPELEDEDIRQALAFAAALVDDKVLPLPHAR"
     gene            1442..>2283
                     /locus_tag="VGR03_06730"
     CDS             1442..>2283
                     /locus_tag="VGR03_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994004.1"
                     /translation="MPVDEYRTLHAKAYPIERDPEPPPVEATLTRVDYDLRIDGELAS
                     GRASLTVDVLKDGWVRVPVPAGLLVREARLDGKLVSLVPGAGGKAGSQLSALLSHSGR
                     AILLLDIAIPVASATGEESITLPSTASGVTRASVQLPRQGVDVRLTGGLLSEKSESAA
                     ESKWIAYGRGNEALTFAWRRKTEDHRATQPLRMRGSLTQLLGLGEDSTSINAEVNLEV
                     TQGAARVARIQIPDKVTINQVLGAMVADWEVKANELSVTFLEPVEQTARFVVTGETRS
                     PREG"
BASE COUNT          458 a          741 c          607 g          477 t
ORIGIN      
        1 cgcggatcgt gactttcagt tcgctgactt cagcacccgg cgacgccccg cgcgacgcga
       61 gaacacctcc cagtcccaga cccagaattg cgccgcccaa aataatcggg tttcttcttc
      121 ccatctgtac ctccctgcca gaattcctgg tcgctgtaag ctgacttcca tctattcctg
      181 aacttcatgg caatgtacgt tgcgctggtc gggcccttgc aggcccctct acaatttgct
      241 agcttaatct tcagagcggg cgtcgtacaa tggcagtata gaagcttccc aagcttcgga
      301 cgtgggttcg attcccatcg cccgctccag ttctgcaaaa acagttacgc ggttgtggtt
      361 cccgctcgca gcttgcaaag acattatcgc actagcggaa gccgtctaat tctgactccg
      421 ttctcagtcg ccgacaccat cgctccctag gcgagatcat ccaagcaaat ccccgacacg
      481 ctgaccagta aactggcaag ctcctccgca cgctgacctt cgatccgaat gcgaaccacg
      541 gatgggctat tcgcgccaga cagcgccagc agaacgtgga agtcggcatc caaagtcacg
      601 acgactcagc cgccctcatg gggcaaactc cagtattttc gtgtcacccg ccgtggcgag
      661 ctctagttct cccacatgga ccgactcgat cccagctctc agtagatggg agactgcgga
      721 acgcgggagg ccttgatcga gccgcagcct catcgggcgt gcggcaacgg caagaccttg
      781 tcgtcaacca aggcagccgc gaaggccagc gcttgccgga tgtcttcgtc ttctagctca
      841 gggtattcgc acttgagtgc ggcgcgatcc ggatagatgg cgagcgcttc aagtactcgg
      901 cggacagtta ggcggaggtc gcggatgcag ggctcgccgt tcatccgagc aggatcagtc
      961 gtgattcgtt cgagtttctc catcttgtcc gccttttctg gacggccggg agcaagtcct
     1021 tagcaaagct cgccgcgcga cggtccatct ctctgagaat acccgactac ccccctcgct
     1081 aagcaagccc tgcaagaaat catccgctcg ccgttctaat agctgagtac cggcagtcgt
     1141 atgtcccggc cgttcgattt ttttatgaag gaattatttt atgaaacaac catcttcgcg
     1201 cagcccgtca tcctatccac tcaacagcac gctccgtccc cgggtcgccc aacgaattca
     1261 cccgctcgtc catcttgaag atgaggaggc tacttctatg cccgcaccac tcgcagcaac
     1321 tctcccgctg aaaaaaaggc ccggatcgcg actcttctct gcttctctgt tcgccctcct
     1381 attcctttct gtcactagca ccttggcgca gaccgctgct cattcgccag gctgggtggt
     1441 tttgccagtc gatgaatacc ggacccttca cgcaaaggcc tatcctatcg aacgcgatcc
     1501 ggagcctcct cccgttgaag ccacgctcac acgcgttgac tacgacctgc gcatcgatgg
     1561 tgaacttgct tccggccgcg caagcctgac ggtcgatgtc ttgaaggacg gatgggtgcg
     1621 cgtcccggtt ccggcgggtc tgctggttcg tgaagcaagg ctcgacggca aacttgtttc
     1681 tctcgtgccc ggcgccggcg gcaaagcggg aagccaactc tccgcgcttc tttcccattc
     1741 gggccgcgcg atcttgttgc tcgatatcgc cattcccgtg gcgtccgcaa ccggcgagga
     1801 aagtatcact ctgccctcga ccgcctccgg cgttacccgc gcgtccgtgc agcttcctcg
     1861 ccagggcgtg gatgtccggc tcacgggcgg cctactctct gagaaatccg aatccgcggc
     1921 agaaagcaag tggatcgctt acggacgcgg aaacgaagcg cttaccttcg cttggcgcag
     1981 gaagaccgag gatcaccgcg ccacgcagcc tttgcgcatg cgcggctcgc tgacgcaact
     2041 cctggggctc ggcgaagact ccacttccat caacgccgaa gtaaatctgg aggtaactca
     2101 gggcgcagcg cgagtcgcac gaattcaaat ccccgacaaa gtcaccatca atcaggtctt
     2161 gggagcaatg gtggccgatt gggaggtgaa ggccaacgaa ctctctgtga cgttcctgga
     2221 accggtggag caaaccgcgc gattcgtcgt gaccggagaa actcgcagcc cccgcgaagg
     2281 aca
//
LOCUS       DASYVO010000199         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_117664, whole genome shotgun sequence.
ACCESSION   DASYVO010000199 DASYVO010000000
VERSION     DASYVO010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117664"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VGR03_06735"
     CDS             <1..1036
                     /locus_tag="VGR03_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEV1994005.1"
                     /translation="RGAACCAPTTGELAVDTIGFGSGTLMSLDTARRVLKIEAQAIQD
                     VLARLDASFEKAVDVLFACKGRVVVTGMGKSGLIGRKISATLSSTGTPSFFLHAAEAL
                     HGDMGMLARGDALLAVSYGGETEEIVRLLEALKRLEMPLVTLTGKVHSTLGEASDVVL
                     DVSVKEEACSMNLAPTASTTVAMAVGDALAISLLERRGFRPDDFAALHPAGSLGKKLL
                     RVEHLMHAGEALPRVSKDTPMPDVFHEMSAKKLGMTTVLEPDGRLAGILTDGDLRRLM
                     EKHRGAVLEMSAADGMTKNPQTIGPHVLASEALNLMEKRKITSIVVVDEARGVLGVVH
                     LHDLWTLELM"
     gene            1247..1807
                     /locus_tag="VGR03_06740"
     CDS             1247..1807
                     /locus_tag="VGR03_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEV1994006.1"
                     /translation="MAKRGKKIPRKLAKRAKPILVLLMDVDGTLTDGSVTLLSQSDGS
                     ALEMKNFDAHDGQGLTLAVSAGLRTGVITGRASAALRRRCKELDIEFVYEKQGHKIAA
                     YEDVLRKTGAKESEVAFLGDDLPDLMVMKRVGLAVAVHNATPEVRRAAHYTTKADGGK
                     GAARELIELILRSKGTWEEMIDKARA"
BASE COUNT          449 a          590 c          632 g          388 t
ORIGIN      
        1 taggggcgca gcatgctgcg cccctaccac cggggagttg gccgttgaca ccatcggatt
       61 tggttcgggg acgctaatga gtctcgacac ggcgcggcgc gtgctgaaga ttgaggcgca
      121 ggcaattcag gacgttctcg cgcgtctcga cgccagcttc gaaaaagccg tggacgtctt
      181 gttcgcctgc aagggccgcg tcgttgtcac cggcatggga aaatcgggcc tcatcggccg
      241 aaaaatctcg gcaacacttt ccagcacggg aaccccgtcc ttcttcctgc acgcggcgga
      301 ggctctgcac ggggatatgg gcatgctcgc ccgcggagat gctctcttag ccgtctctta
      361 tggcggcgag acggaagaga ttgtccggct gttggaggcg ctgaagcggc tggagatgcc
      421 cctcgtcact ttgaccggca aagttcattc cacgctgggg gaagcgagcg acgtcgtgct
      481 ggacgtaagc gtgaaggaag aagcgtgttc catgaatctt gcaccgacgg cgagcacgac
      541 cgtagcgatg gcggtgggcg acgcgctggc aatctcgctt ctcgaacgcc ggggttttcg
      601 ccccgatgat ttcgccgcgc tgcatcccgc cggaagcctc ggcaagaaac tgctgcgcgt
      661 cgaacatctc atgcacgcgg gagaagcgct ccctcgcgtt tcgaaagaca cgccaatgcc
      721 ggacgttttc catgaaatgt cggccaagaa gctcggaatg accaccgtcc tggaaccgga
      781 tggacgcctg gcgggaattc tgacggatgg cgaccttcgc cggctgatgg agaaacatcg
      841 cggagccgtt ctggagatgt ccgcagcgga tggcatgacg aagaatccgc aaacgattgg
      901 gccacatgtg ctcgcgagtg aagcactgaa tttgatggag aagcggaaga ttacttccat
      961 tgttgtggtg gacgaagcaa gaggcgtgct cggcgtggtg catctgcacg atttgtggac
     1021 gctggagctg atgtagagac aaaagggaac gctggcgcag tcagtcactc tcaacaccgg
     1081 cctcgcggcc cgtgatttca gatgtgggtg ttctgatgta gtggcgggtc ttcagacccg
     1141 atctttcttt ctcgtcgtag gggcgcagca tgctgcgccc ctactcggca acgagtgcga
     1201 gcataagact cgattcggca aacgcaaaga aaaagaggaa aagaaattgg ccaagcgagg
     1261 gaagaaaatt ccgagaaaac tggcgaaacg tgccaagcca attctcgttc tgctgatgga
     1321 cgtggacgga acactgaccg atggcagcgt tacgctactg tcgcaatcgg atggttccgc
     1381 gctggagatg aagaattttg acgctcacga cgggcagggc ctcacgcttg ctgttagcgc
     1441 cgggttgcgc actggcgtca tcaccggccg cgccagcgcg gctctgcgca gacgctgcaa
     1501 agagctggat atcgagtttg tctacgagaa gcaggggcac aagatcgcgg cttacgaaga
     1561 tgtcctgcga aagactggcg ccaaggaatc ggaagtcgcc tttcttggcg atgacctccc
     1621 tgacctgatg gtaatgaagc gcgtcggcct agccgtcgcc gttcacaacg ccaccccgga
     1681 agtccgccgc gccgcccact acaccaccaa agccgatggc ggcaaaggcg ccgcccgcga
     1741 actcatcgaa ctcatcctcc gctccaaagg cacctgggaa gagatgatcg acaaagcccg
     1801 cgcctgaacg cccaccttct ccccccgctt cccggccccg gtccgctgcg aacctgctat
     1861 cccgcagccg gcaaacgact tacagctaac ctagagaagt gggccagttt ccgattaccc
     1921 gacgcaatat aaaagggcgc cccatttgga ataggttttg agtgttcgct cgaacccctc
     1981 cagaaaggag caccctgtat ggatagcgtg aagtatatcg gcatggatgt tcacaaggaa
     2041 gcgatctcca tcgcggttc
//
LOCUS       DASYVO010000200         9943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_59772, whole genome shotgun sequence.
ACCESSION   DASYVO010000200 DASYVO010000000
VERSION     DASYVO010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9943
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59772"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VGR03_06745"
     CDS             complement(<1..842)
                     /locus_tag="VGR03_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994007.1"
                     /translation="MDQYRTAESWTSVPGTIAPADGNLASGVAPASLSAFHANANQSE
                     SRQQDAGATPAPIDLGPNNERLEDVKPELVHALRELVRQYRQEGIVARRHEIRRIRQA
                     RLFWQGLQYAWWNPNDMNWHLPFEQRSKDDRELEEMPRYQFVTNFYQGFGLSFIAVLS
                     QDVPSVRFYPQSAQSLEDIAAARAASDVAQLVEQNNRVEQLLTSIGYFLWTDGKLGAY
                     VRYVADGQRFGFHEENVLEALEIPLGEDVYVCPQCGKEIGQSKIENRNSEDTDDASAV
                     GAQHA"
     gene            complement(820..1122)
                     /locus_tag="VGR03_06750"
     CDS             complement(820..1122)
                     /locus_tag="VGR03_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994008.1"
                     /translation="MNRMKDWIEKVFRGRYVGLLEADVARLRAENRALMNSLLGTAGF
                     PPVEFPEAPKPQPLPRLRKRSWHQLQAWRQTEANRDSAESFDERTSERANGPVPHS"
     gene            complement(1133..2626)
                     /locus_tag="VGR03_06755"
     CDS             complement(1133..2626)
                     /locus_tag="VGR03_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994009.1"
                     /translation="MFGGAAGPGKTKALLWEAIRQATNVKGCDTLLMRRTYPELEASL
                     LAYFRRDVRRELYKSYNESKHLVTWTNGSTTRFGYCRNENDVYQYQGAEFLFIGIDEL
                     THFTMKQWQFLTSRNRCPVPNSFCNMAGATNPGNVGHAWVKALWVDHIPPSGFERTDM
                     YNALDYHFIRARIDDNPIYANDVEYRRTLEALPEHLRRAFLDGDWNVFAGQYFDIFDI
                     GRHTARPEELRLEAWWPRWISIDWGFQHPSAVYWHCAVPGSFGFQPAGFVSGAEGENR
                     RQDPGATNGSRMVTYREFVQNGLSPRMLAQAIAERSGRERISEVFLSPDAFAHRTSEA
                     SIAEQLGDVLVSNGLPRPAPADDDRIGGWQLMYQMLESNAWVITDNCAKLIECMPQLV
                     RDTGRIEDIRKVDGDDPADAARYGLVSGGRLAGVGPAFVPSRAGQSPPLYQPVSTPHF
                     VTGMPLGEQIARQVSATDQTSRAIHYQRLEAEAKKQFRPKKLPRRHW"
     gene            complement(2782..2946)
                     /locus_tag="VGR03_06760"
     CDS             complement(2782..2946)
                     /locus_tag="VGR03_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994010.1"
                     /translation="MANAKTVFIGEVTQIAGIAAVVAGVVLSMHHWPAAAALIGGGAA
                     FYVGKKLRAQ"
     gene            complement(2960..3538)
                     /locus_tag="VGR03_06765"
     CDS             complement(2960..3538)
                     /locus_tag="VGR03_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994011.1"
                     /translation="MTHRKNQRKTNGHAGPHDRTNGSGDRKAVNGHGPASTERGGTPV
                     KRDRAASDDASGATVARAAGSATAPGTVRKAYAEAAPPHSGKKNGKMKSKDKGKNGQS
                     AGLKDFHLVGATDFSAMQDGAAYVNAVAARVDLVGASVRLVCSEDEKIAKAELDRVRD
                     MKFGKAAAGTNMPESPTVIWDVPGINIRRNEG"
     gene            complement(3548..3790)
                     /locus_tag="VGR03_06770"
     CDS             complement(3548..3790)
                     /locus_tag="VGR03_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994012.1"
                     /translation="MSDGERSLWRAVLNQAYEDAEMQAIGNEPETEPVESLRARRYLR
                     ADSPFEAANLELVCEFAELPADRVFLWARQRYSLAA"
     gene            complement(4106..4816)
                     /locus_tag="VGR03_06775"
     CDS             complement(4106..4816)
                     /locus_tag="VGR03_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994013.1"
                     /translation="MATTTWVAGLYADLFAYKNADARATTQQLEEEQSRAALVPALPR
                     MEWDETFRWQGYCDARVSVLALEQETGLLPVLPRPSRDEVEWQRWFGSQFQNQTQEID
                     SGLFPILPLRAWEEPEWQKWADSRIQQITSEQDTGLLPIISLPPPTLGWDDWDWNKNF
                     TEKRLWFESEALVGAPFLPSTSWLEESAAAFAGSSGSLAAMVMMDDFVNAPSGGTTFG
                     PAPRGRSVRLGFSISAFR"
     gene            complement(4820..5308)
                     /locus_tag="VGR03_06780"
     CDS             complement(4820..5308)
                     /locus_tag="VGR03_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994014.1"
                     /translation="MPFMYSASSQQNTNASANTDTLLADLKTASANAGLRAYLQKLQV
                     GSYATPADNALRIRLQRLATLGTYASGTALTAAPIVPDAPAAASLSSTLPTQGTGTLN
                     AVPLLQLAFNQRGTGLWAAFTADEAVGISGSNGAVNGIMVLNSQSTGTSVPVNYKMIF
                     SE"
     gene            complement(5330..5614)
                     /locus_tag="VGR03_06785"
     CDS             complement(5330..5614)
                     /locus_tag="VGR03_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994015.1"
                     /translation="MGKILEFGDDSRLKGYSGRVTHAILPLERTFCTNCGRPWGWASE
                     DSSKHIAAAEIVVFCGECFSALNAGNDNGPMQCIPANEMRSLGLVEENIR"
     gene            complement(5616..5930)
                     /locus_tag="VGR03_06790"
     CDS             complement(5616..5930)
                     /locus_tag="VGR03_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994016.1"
                     /translation="MISRWVAHCVAWLRFVFKLTRLLDTPLWRVADGAVMQVMQHHEL
                     HKACKNEELTSFCPRCGQMPDGDRRMEEARENFLAMWKGDAPKRSELDFALVWAYFRR
                     KL"
     gene            complement(5932..6243)
                     /locus_tag="VGR03_06795"
     CDS             complement(5932..6243)
                     /locus_tag="VGR03_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994017.1"
                     /translation="MRTDPQGRAIQIGRYLSAPAVLNIGTVSASIQLNQNRHYRLWPS
                     VDMFFQFGASGVSATTNSHPATWKLDYLHYTDDTNTTLAAVVSSGTGVLFISEIDTQG
                     A"
     gene            complement(6247..6618)
                     /locus_tag="VGR03_06800"
     CDS             complement(6247..6618)
                     /locus_tag="VGR03_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994018.1"
                     /translation="MPSYQNVTPPISLMPGDVGFSFNNEAFPGGAQAGSQFALPSYAG
                     QPDVGTAVRWQTIFGGAPTAVSLVLQGAMADIDAEYQTIDTTVATTGEARTVTGVQSK
                     FLRIKFNSSTGGSGLTAKLLV"
     gene            7034..7258
                     /locus_tag="VGR03_06805"
     CDS             7034..7258
                     /locus_tag="VGR03_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994019.1"
                     /translation="MRSKYRLALYLAPLALAAFVPSGPYTGPNTRLGLATMVIWHNAD
                     HHGQMTLHLRLNGIVPPASRPNPPEAKDLY"
     gene            7533..8048
                     /locus_tag="VGR03_06810"
     CDS             7533..8048
                     /locus_tag="VGR03_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994020.1"
                     /translation="MIAFTFFSIADYWTSFGWGGQHLAANEASAVGTLRRLSSAEEKL
                     ADPTRSGSAMKARYGTIEDLRKNRLIDDDIEAGRSRKGYVFREVVDPERKQFLLYAVP
                     AHLPPAESPPDWVHFLPGWALYYNAWRRDETHGTGFRSFAVDETGAIRYTIVPATGPV
                     THEEIAHWEKL"
     gene            8102..8908
                     /locus_tag="VGR03_06815"
     CDS             8102..8908
                     /locus_tag="VGR03_06815"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEV1994021.1"
                     /translation="MTDALAATPRLANIRLHPIKSLDPVHVQEARIGPGGGLEFDRAW
                     ALYSADGQWVNGKRTAAIHLIRAAYVPDISSITLSVPGERRKIPTKTFAFPADTAAAS
                     QWFSNFFEQPITVRHSPEGFPDDPIANGPTIVSTASLQALCDLFKGMDLDGARQRFRT
                     TLEIEGVPAFWEDQLFGEEERSAVRFRIGDVNFEGSNPCARCPVPPRDPHTGEIIPGF
                     QKIFSDYRRSHIPAWCPEARFDHYYRLATNTLVASSEARKVLRVGDVLHL"
     gene            9040..>9943
                     /locus_tag="VGR03_06820"
     CDS             9040..>9943
                     /locus_tag="VGR03_06820"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV1994022.1"
                     /translation="MTLANRGIAAARNLQRWFKRRMLQQPWMMEEFASPILQESLLLD
                     TARCDAYREAIHRTVKPGDVVVDLGAGTGLLSFFALQAGARHVYAIEMSDIADAAAEL
                     IEANGFQDRITLIRKNSKKVRLPERCDVLVTETLSAFCFDAENIIEFVADARERFLKS
                     GARIIPESADTFLMPFYSDAFGAGRLPPRHYDLDFKPFRKRLFKSYGIVRASGQPFVA
                     LSQPAPCYQIDFRKDSQDPGKTFTPFRIAADGRLDGFLGWFEARLCEGITLSNSPYLP
                     LTHWWQLYLPILEQPHYRAGQTILL"
BASE COUNT         1868 a         3181 c         2788 g         2106 t
ORIGIN      
        1 gcatgctgcg cccctacagc cgaggcatcg tccgtgtctt ccgaatttct attttcaatt
       61 ttcgattgtc caatttcctt gccgcactgc gggcaaacat agacatcttc gcctaaggga
      121 atttccaaag cttcgagcac attttcttca tgaaagccga agcgctggcc atcggcaacg
      181 tagcgcacat atgcgccgag ctttccatcg gtccacagga aatagccgat cgaagtgagc
      241 aactgctcga cgcgattgtt ctgctccacc aactgggcga cgtcgctagc ggcgcgcgct
      301 gcggcaatgt cttccagcga ttgcgccgac tgcgggtaga aacgcacgct tggcacgtcc
      361 tgcgaaagca cggcgatgaa cgaaaggccg aaaccttgat aaaaattcgt gacgaattgg
      421 tagcgtggca tctcttcgag ctcgcggtcg tccttagagc gctgctcaaa cggcaaatgc
      481 cagttcatgt cgttgggatt ccaccaggcg tattgcaagc cctgccagaa gagccgcgcc
      541 tggcgaatcc gccgaatctc atgccgcctc gcaacgatgc cttcctggcg gtattgccga
      601 accagttcgc gcaaggcgtg cacgagttcg ggtttcacat cctcgagccg ctcgttgttc
      661 ggacccagat cgatcggcgc aggggtagcg ccagcatctt gctggcggct ttcagattga
      721 ttcgcgttgg cgtgaaacgc cgacaaagat gccggcgcta cgcccgaggc gaggttgcca
      781 tcagcaggcg cgatcgttcc ggggacactc gtccaggatt cagctgtgcg gtactggtcc
      841 attcgccctc tcagacgttc tctcgtcaaa actttcggcg ctgtcccgat tcgcttcggt
      901 ttggcgccac gcctggagct ggtgccagga gcgctttctc aaccgcggca atggctgcgg
      961 cttgggcgcc tcgggaaact ccaccggagg aaatcccgcc gtgcccagca gagaattcat
     1021 cagcgcgcgg ttctccgccc gcagccgcgc cacatcggct tcgagcagac cgacgtaccg
     1081 tccgcgaaac accttttcga tccagtcctt catacggttc atcgaccttg cctcaccaat
     1141 gtctccgcgg cagcttcttc ggccggaatt gctttttcgc ttcggcttcg aggcgctggt
     1201 aatgaatcgc acgcgaagtt tgatcggtcg cgctgacctg ccgcgcgatc tgttcgccca
     1261 gcggcatgcc ggtgacaaaa tgcggagtgc tcaccggttg atagaggggc ggggattgcc
     1321 ccgccctgga cggcacaaag gccggcccaa caccggcgag tcttccgccg gagaccaatc
     1381 cataacgcgc ggcgtcggcg ggatcatcgc cgtccacttt gcgaatgtcc tcgatccggc
     1441 ccgtatcgcg cacgagctgc ggcatgcatt cgatcagctt cgcgcaattg tcggtgatga
     1501 cccaggcatt cgattcgagc atctgataca tcaactgcca gccgccgatg cggtcgtcat
     1561 cggctggcgc cggccgtggc aagccgttcg agaccagcac gtcgccgagc tgctccgcga
     1621 tcgatgcttc cgaagtgcga tgcgcgaacg cgtccggtga tagaaagact tcgctgatgc
     1681 gctcgcggcc gctgcgttcc gcaatggcct gcgccagcat gcgcggcgag aggccgttct
     1741 gcacgaattc gcgatatgtg accatgcggg agccgttggt agcgccggga tcttgccggc
     1801 ggttttcacc ctcggctccg ctgacgaacc ccgccggctg gaagccgaag ctacccggaa
     1861 ctgcgcaatg ccaatacacc gcgctgggat gctgaaaacc ccagtcaatc gaaatccacc
     1921 gcggccacca cgcctctaga cgcaactcct cgggccgcgc ggtgtgccgc ccgatatcga
     1981 agatgtcaaa gtattgcccc gcaaaaacgt tccaatctcc atcgagaaac gcgcgccgca
     2041 gatgctccgg cagcgcttcg agcgtccggc gatactccac atcgttagcg tagatggggt
     2101 tatcgtcgat ccgcgcgcgg atgaagtggt agtcaagcgc gttgtacata tccgtgcgct
     2161 cgaatcccga cggcggaatg tgatcgaccc acaacgcctt gacccacgcg tgcccgacgt
     2221 ttcctgggtt cgtcgcgccc gccatgttgc agaaactatt tggcacgggg cagcggttgc
     2281 gcgacgtcag gaattgccac tgtttcatcg taaaatgcgt cagctcgtcg atcccgatga
     2341 acagaaactc cgccccttga tactggtaga cgtcattctc gttgcggcag tagccgaagc
     2401 gcgtggtcga gccgtttgtc caggtcacca ggtgcttcga ttcgttgtaa ctcttgtaca
     2461 gctcgcgccg tacgtcgcgt cggaaatacg ccagcagcga cgcttccagc tcgggatacg
     2521 tgcgccgcat tagcaacgta tcgcagcctt ttacgttggt tgcctgacga atcgcttccc
     2581 aaagcagagc tttcgtcttg cctggacccg cggcacctcc aaacaagcga tatttcgccc
     2641 aagaattgtg aaattgtttc tgccgatcaa atggatcgta atcctcaggg cctatccgta
     2701 gcgcagatcc agcgcggcta ctcgtgttcc gcgatcggct ttgccagttg gccatcttgg
     2761 tcgcgccgga cgacacttgc gttactgcgc gcgcaacttt ttgccgacat aaaacgccgc
     2821 gccgccgcca atcaatgccg ccgcggccgg ccagtgatgc atgctcaaca ctacccccgc
     2881 cacgaccgca gcaattcccg cgatctgcgt tacttcaccg atgaagacag tctttgcatt
     2941 cgccattggc actcctcccc tatccttcat tccgtcggat gtttatcccg ggcacgtccc
     3001 agatcacagt gggactttcg ggcatatttg tccctgccgc ggctttgccg aatttcatat
     3061 cgcgcacgcg gtccagctcg gccttggcga tcttttcgtc ctctgagcaa accagccgta
     3121 cgcttgctcc cacgagatcc acacgtgcag caaccgcgtt gacgtaggct gcgccgtcct
     3181 gcatcgcgga gaaatccgtc gcgccgacaa gatgaaaatc cttcaggccc gcactttgcc
     3241 cgttcttgcc cttgtcctta ctcttcatct ttccgttctt tttccccgaa tggggcggcg
     3301 ccgcctcagc gtacgccttg cggaccgtgc cgggcgcagt ggcggagcct gcggcccttg
     3361 caacggttgc tccgctagcg tcgtcagacg cagctcgatc ccgcttcacc ggggtcccgc
     3421 cccgctccgt ggaggccgga ccatgaccgt tgaccgcttt gcggtcacca cttccattgg
     3481 tgcggtcgtg cggtccggca tgcccgttgg ttttgcgctg attttttcgg tgtgtcacga
     3541 tattccatca agcggcgagc gaatagcgct gccgcgccca caaaaagacg cggtccgcgg
     3601 gtaactccgc aaactcgcag acgagttcga ggttggctgc ttcaaaagga ctatccgccc
     3661 gcaagtatcg gcgcgcccgc agggattcga ccggctccgt ctcgggttcg ttaccgatcg
     3721 cttgcatctc cgcatcctcg taagcctggt tgagcaccgc cctccacagg ctgcgttctc
     3781 catcgctcat gaatcctccg gcagtaaaga gactctgggt cctgcaaaga ttcctcgaga
     3841 tcgggcgctg gactcgatgt gtttcgcgtt atttcttgtt ttgagtttgg ttttgtctct
     3901 gttgcccgtg cctgcatgta tcccaaaacg ctctgggaag ccgtttaccc acaatcccag
     3961 aaccgctcca ttgatcgcgt tctcgtgcga ccggcaaaat tgcgagcccg ctgcggcccg
     4021 gcgtatacat cgcacccaca catgcgttga attccctggg cggacgtagg cgagacagtg
     4081 cggcatggag aactcagatg gcttgctaac gaaatgcgga gatcgaaaac cccaatcgta
     4141 cgctgcgtcc gcgcggcgca ggaccaaacg tggtgccgcc cgacggcgcg ttcacaaaat
     4201 cgtccatcat caccatcgcg gctagcgagc cggaactccc tgcaaacgct gctgcgctct
     4261 cttcaagcca gctcgtggac ggcaagaacg gagcgccaac cagcgcctcg ctctcaaacc
     4321 agagccgctt ttccgtgaaa ttcttgttcc aatcccaatc gtcccagcca agtgtcggcg
     4381 gcgggagcga gatgatcgga agcagcccgg tgtcctgctc cgaggtgatt tgttgaatac
     4441 ggctgtccgc ccacttctgc cactccggtt cctcccaagc gcgcaagggg aggatgggga
     4501 agagcccgga atcgatctct tgtgtttggt tttgaaactg cgacccgaac cagcgctgcc
     4561 attcgacttc gtcccgactc ggtctcggaa gtaccggcaa caaaccggtt tcttgttcga
     4621 gcgcgaggac acttacgcgg gcatcgcagt acccttgcca gcggaaggtt tcgtcccact
     4681 ccatcctagg gagggcgggc acgagcgccg ctcggctctg ctcttcttcc aactgctggg
     4741 tcgttgctcg cgcatcggcg ttcttgtagg cgaacagatc cgcgtagagg cccgcaaccc
     4801 aggttgttgt cgccacacgt tactcgctga aaatcatctt gtaattgacg ggcacgctcg
     4861 tgccggtgga ctggctgttc agcaccatga tgccgttgac ggcgccgttc gatccgctga
     4921 tgcccacggc ttcgtccgcg gtgaacgcgg cccacaagcc tgtgccgcgc tggttgaacg
     4981 ccagttgcag cagcggcacg gcattgagag tgcccgtgcc ttgagttggc aacgtcgagg
     5041 aaagcgaagc ggccgcaggc gcgtccggca cgatcggcgc cgccgtgagc gccgtgcccg
     5101 aagcgtaggt gccgagcgtc gccaggcgtt gcaggcggat gcgaagcgcg ttgtccgcgg
     5161 gtgtggcgta cgagccgacc tgcagttttt gcaggtacgc gcgcaacccg gcgttcgcgc
     5221 ttgcggtctt caagtccgcg agcaaggtat cggtgttggc ggacgcgttg gtgttttgct
     5281 gcgaggacgc ggaatacatg aatggcatga atctcctccg atgttgcagc taccgaatgt
     5341 tttcttccac caggcccaga cttctcatct cgttagcggg gatacactgc attggcccgt
     5401 tgtcattgcc ggcgttcaag gccgagaagc actccccgca aaaaaccacg atctccgctg
     5461 cggcaatgtg tttgctcgaa tcttcggaag cccagcccca gggccggcca caattggtgc
     5521 aaaatgtgcg ctcaagtgga agaatcgcgt gagtgacgcg gccgctgtag cccttgagcc
     5581 gcgagtcatc cccgaactcg agaatcttgc ccacgttaaa gcttccgccg gaagtacgcc
     5641 cagaccagcg cgaaatccaa ttcactgcgt ttcggagcgt cgcccttcca catcgctaga
     5701 aaattctcgc gggcttcttc catgcggcgg tcgccgtccg gcatctgccc gcacctgggg
     5761 cagaaacttg tcagctcttc gttcttgcaa gctttgtgca gctcgtggtg ctgcatcact
     5821 tgcatcacag cgccatccgc tacgcgccag agcggcgtat ccagcagccg cgtgagcttg
     5881 aagacaaacc gcagccaagc gacacagtgg gcgacccagc gggagatcaa ctcaggctcc
     5941 ttgcgtatcg atttcgctga tgaacagcac gccggtgccg gagctcacca ctgccgccag
     6001 cgtcgtgttc gtgtcgtcgg tgtagtgcag gtaatccagt ttccaagtgg caggatggct
     6061 gttcgtcgtg gccgatactc cggaagcgcc gaactgaaaa aacatatcca cactgggcca
     6121 cagccggtag tggcgattct gattcaactg gatcgatgcg ctgaccgtgc cgatgttcaa
     6181 caccgccggc gcgctcaaat agcgcccgat ctggatcgcc cggccctgcg gatcggtccg
     6241 catgcgttac accaggagtt tcgcggtcag tccgctgccg ccggtggacg aattgaactt
     6301 gatgcgcagg aattttgact gcacgccagt cacggtgcgc gcttcgccgg tggtcgccac
     6361 ggtggtgtcg atggtctggt attcagcgtc aatgtccgcc atggcccctt gcaacaccag
     6421 gctcaccgcc gtcggcgccc cgccgaaaat ggtctgccag cgcaccgccg tgccaacatc
     6481 tggctggcct gcatagctcg gcagcgcaaa ctgactgccc gcctgcgcgc cacctggaaa
     6541 cgcttcgttg ttaaaactga atcccacgtc gcccggcatc agggaaatcg gcggcgtcac
     6601 attttgatag cttggcatct tggctccttc tgtggcacac ccacactagc tcgccccgag
     6661 tctcgcgggg atttacttcg ttgtagcggc cgcctggcgt tcaccccgac tcttgtcggg
     6721 gaggccgagc gcttttgcgc aaggaaacac agcacggcgc gaaagtcttg ctagcacggg
     6781 ctttagctgg tgcagaggta atcttggaga gtcctgcgag agagggccga gggcagcccc
     6841 aaaaagcaaa acgccccggc attgccgaag cgtggctttc tacagaagca agtgtatcac
     6901 accaatatgt attgtcaata aaaacttttg tttttcgcaa agattttttt taaccccgca
     6961 ttttcaatca cttccctgct cgttgatttt caaaatgaga ggggtgatag acttctcccg
     7021 caggagttca ccgatgcgat caaaataccg gctggcttta tacctcgcgc cgctggcatt
     7081 ggcggctttc gtgccctcag gcccatacac cggcccgaac acgcgcctcg gcctcgccac
     7141 tatggtgatc tggcacaacg ccgaccacca cggccagatg accctccatc tgcgcctgaa
     7201 cggcatcgtc ccgcccgcca gccgccccaa tcctccggaa gcgaaagacc tttactaaga
     7261 tgatgccaga tccaggcgga ctcgtttcca ttctgcttct ctttcccatc atcatctttg
     7321 gttcgcggat cgcccgagct tcgccgatcg aagagactcg cgacaggcgg atccggactg
     7381 gcattgtgct tgggatctgt gtgttcctca tggcaatcct gccactgttg ggtcccatcg
     7441 cgttcttgat agtcctcttt tatggaattt tttgcggtat tcaaatcatg aggcggggag
     7501 agggccggag acgttggcta atcggcgcct ttgtgatcgc attcacgttc ttttcaatcg
     7561 ccgattattg gacctccttt gggtggggtg gacagcacct cgccgccaat gaagcttccg
     7621 ccgtggggac gctccgcaga ttatcttctg ctgaggagaa actcgccgac ccaacacgta
     7681 gcgggtcggc aatgaaagcg cggtacggaa cgatcgaaga tttgcgcaaa aaccggctca
     7741 tcgacgatga tatagaagca ggtagatctc gcaaaggtta tgtcttccgc gaagtcgtcg
     7801 accctgaaag gaaacagttc cttctttatg cggttcctgc gcaccttcct ccggccgaat
     7861 ctccgcccga ctgggtccac ttcctgccgg gctgggcact gtattacaac gcttggaggc
     7921 gtgacgaaac gcatgggacg ggctttcgaa gtttcgccgt ggatgaaaca ggagccattc
     7981 gctacacgat cgtccccgcc accgggccgg tgacgcacga agagatcgca cactgggaaa
     8041 aactttagaa gtaggctgga ttcccggcct tgggccacgc aacaattggt agactaagac
     8101 catgactgat gcgctcgcgg cgacgccgcg actggcgaac atccgccttc atcccatcaa
     8161 atcgctcgat ccggtgcatg tgcaggaagc acgcatcggc ccgggcggcg ggctcgaatt
     8221 cgaccgtgcg tgggcgctct actccgctga cgggcaatgg gtgaacggca agcgcaccgc
     8281 ggccatccac ctgatccgcg ccgcgtacgt gccggacatc agctctatca cgctttcggt
     8341 tcccggtgag cgccgcaaaa tcccaaccaa gacatttgcg ttcccggccg acacggcagc
     8401 cgcctcccaa tggttttcca acttcttcga gcagcccatc accgtccgcc attctcccga
     8461 aggatttccg gatgacccca tcgccaacgg ccccacgatt gtttccacgg caagcttgca
     8521 ggctctttgc gatctgttta aaggcatgga cctcgacgga gcgcgccagc gcttccgcac
     8581 gacgctcgag atcgaaggcg tgcccgcgtt ctgggaagat caactcttcg gcgaagaaga
     8641 acgcagcgcc gtgcgtttcc gcattggcga tgtgaatttc gagggcagca atccctgcgc
     8701 gcgttgcccg gttcccccgc gcgacccgca cacgggagaa atcattcccg gcttccagaa
     8761 aatcttcagc gattaccgcc gctcgcacat cccggcgtgg tgccccgaag cccgcttcga
     8821 ccactactac cgcctggcca ccaacacgct cgttgcttcc agtgaagccc gcaaagttct
     8881 gcgcgtcggc gacgtgcttc acctgtagtg gccggtcttt agaccggtgc tttttctgtg
     8941 tccagatgcc ttggtgtcgc ccgctcgcgc ccgtatacta cctcgcgtcc cgcttccccg
     9001 ccgcaatctg ttcgccgcgg cttttgcggg aactctctca tgacccttgc caatcgtgga
     9061 atcgctgcgg cacgcaacct ccagcgctgg ttcaagcgcc gtatgctgca gcaaccgtgg
     9121 atgatggaag agtttgccag cccgatcctg caggaaagcc tgctcctgga cacggctcgg
     9181 tgcgatgcct atcgcgaagc gattcaccgc accgtgaagc ctggcgacgt ggtcgtggat
     9241 ctcggtgcgg gcaccggcct gctttccttc ttcgcgctgc aggccggcgc gcgccacgtt
     9301 tacgctattg aaatgagcga catcgccgat gccgccgccg aactgatcga agccaacggc
     9361 tttcaagacc gcatcacgct gattcgcaag aactcaaaaa aagtgcgcct gccggagcgc
     9421 tgcgatgtcc tcgtcaccga gacgctcagc gctttctgct tcgacgcgga aaacatcatc
     9481 gagttcgtcg ccgacgcgcg cgagcgcttt ctgaagtccg gtgcgcgcat catcccggaa
     9541 tccgcggaca cattcctgat gccgttttat tccgatgctt tcggtgccgg gcgcctcccg
     9601 ccccgccact acgatctgga tttcaagccg ttccgcaaac ggctcttcaa gtcctacggg
     9661 attgtgcgcg cctccggcca accgttcgtc gcgctcagcc agccagcgcc gtgctatcag
     9721 atcgattttc gcaaggactc acaggatccc ggaaaaacat tcactccgtt tcgcatcgcc
     9781 gccgatggcc gcctggatgg cttcctcggc tggttcgagg ctcgcctctg cgagggcatc
     9841 acgctcagca attcgccgta cttgccgctc acgcattggt ggcagctata tctgcctatc
     9901 ctcgagcagc cccactatcg cgccggccaa accatcttgc ttt
//
LOCUS       DASYVO010000201         2398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_245268, whole genome shotgun sequence.
ACCESSION   DASYVO010000201 DASYVO010000000
VERSION     DASYVO010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245268"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..584
                     /locus_tag="VGR03_06825"
     CDS             198..584
                     /locus_tag="VGR03_06825"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HEV1994023.1"
                     /translation="MPETLSTLESKRSSLLEEISQLGDFRSGSVSTVVRRCGQSNCHC
                     ARPQDPGHGPNFRLTYKLKGKTVTETPPHPAAWRKAKREMAQFRKFQGLIRAWVEVNA
                     QICHRRPLEAEATPEEKKRRKPSRAK"
     gene            659..1909
                     /locus_tag="VGR03_06830"
     CDS             659..1909
                     /locus_tag="VGR03_06830"
                     /inference="COORDINATES: protein motif:HMM:NF033572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
                     /protein_id="HEV1994024.1"
                     /translation="METATRGALHRAGAAVLKELLAAPAEFLGQVPCRCGQQARYHEM
                     RPKQWVTMLGPVETERPYYVGPHCRQGQSPRDQELDVEGTEYSPGVRRMMAVVGSATS
                     LEPGREQLELRAGLQVTTQAVERPAATIGAAIAAPEQAAIQQGVPAELPAVEGPEIPV
                     LYIERDGTGAPMVSSATEGRAGKQGEQARPREVKLGCVFTQTTVDARRRPVRDEASTP
                     YTGGIETAQAFGRRMSVQAYRRGWNRAKKKVVLGEGALWIRNLTEEHFPEAIQIVDLY
                     HARQHLGELSAKLGPTDQRRRTQQTQPWENKLKRGQSGSLVRDLRSLSPATIEAAEGV
                     RREADYFAANAQRMRYPAFRRQHLFVGSGVIEAGCKTIVGTRRKRSGMFWTVRGANAI
                     IALRCHRLSGEFENYWESRRPRAA"
     gene            complement(2159..>2398)
                     /locus_tag="VGR03_06835"
     CDS             complement(2159..>2398)
                     /locus_tag="VGR03_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994025.1"
                     /translation="LLLFFRLTLKNLDKRILEHSGWMQLGATLAVCGLLVHSFIDFNL
                     HIPANAAWFSACVGMAQRNLVSSRREGKVDARWVN"
BASE COUNT          498 a          705 c          765 g          430 t
ORIGIN      
        1 tgcggtcgga tggcggatta tcattggagc agcggcatca tgttacccga gtcctgaact
       61 ttatcttcta aagtatcctc aaaggcaatt tatgaggttg gcgggggtgt gcgacataaa
      121 tatgggtctt aggggtttcg ctttttttgt ggctttgatc tggacgtata tgatacgctc
      181 agaatccagg agggactatg cccgaaacac tctcgacttt agaaagtaag aggagttcgt
      241 tgctcgaaga gatttcccag ctgggtgatt ttcgcagcgg ttcggtcagc accgtggtcc
      301 gacgctgcgg ccagtccaac tgccactgcg cccggcctca ggacccaggc catgggccca
      361 acttccgcct cacttacaaa ctgaagggca agacggtcac cgaaacaccc ccccatcccg
      421 ccgcttggcg caaagccaag cgggagatgg cccagttccg caaatttcag ggactcatcc
      481 gggcctgggt cgaggtcaac gcccagatct gtcaccggcg cccgctggag gccgaagcca
      541 cgccggagga aaaaaaacgg cgcaagccat cccgcgcgaa gtagctggcg aagtcgatca
      601 tctcctgcag gtggtcttta cgggtctgcg ctcgagcggt cgcctggact tggaagcggt
      661 ggagacggcc acgcgagggg ccttgcaccg ggccggggcc gcggtgctga aggagttgct
      721 cgccgcgccg gccgagttcc ttgggcaagt tccttgccga tgcgggcagc aggctcgcta
      781 ccacgagatg cgtcccaagc agtgggtgac gatgttgggg ccggtggaga ccgagcgtcc
      841 ctactacgtt ggtccgcact gccgtcaagg ccagagtccg cgcgaccagg aactggatgt
      901 ggagggcacg gaatattcac cgggggtgcg gcggatgatg gcggtggtgg gcagcgcgac
      961 ctcgttggag ccagggcggg aacaactgga actgcgggcc gggctgcagg tcacgaccca
     1021 ggcggtggag cggccggccg caaccatcgg ggccgccatc gccgcgccgg agcaagcggc
     1081 catccagcag ggcgtgcccg ccgagttgcc ggcggtggag gggccggaga ttccggtgct
     1141 ctacatcgag agggatggca cgggggcgcc gatggtgagc tccgcaaccg aggggcgggc
     1201 cgggaagcag ggcgagcagg cgcgcccgcg cgaggtgaaa ttaggctgtg tcttcaccca
     1261 gacgacggtg gatgcacgcc gccggccggt gcgcgacgaa gcctccaccc cttacaccgg
     1321 aggcatcgaa acggcccaag cctttgggcg acggatgtcc gtccaagcct atcgtcgagg
     1381 ttggaaccgg gccaagaaaa aagtggtgct cggtgagggc gctctttgga tccggaacct
     1441 gaccgaggag cacttccccg aggcgattca gattgtcgat ctttaccacg cccgccagca
     1501 cttaggggaa ctgtcggcga agctcgggcc caccgaccag cgccggcgca cccaacagac
     1561 ccagccatgg gaaaataaac tgaagcgcgg gcaaagcggg tcgctggtga gggacttgcg
     1621 atccttgtct ccggccacga tcgaggccgc cgaaggggtg cgacgcgagg ccgattattt
     1681 cgccgccaac gcgcagcgca tgcgctatcc cgcctttcgg cgtcagcatc tcttcgtggg
     1741 atcgggcgtc atcgaagccg gttgcaagac cattgtcggc acccgccgga agcgctcggg
     1801 aatgttctgg accgtccgcg gggccaacgc catcatcgcc ctgcgctgcc atcgcttgag
     1861 cggggaattc gaaaactact gggaaagtcg ccgtccccgc gccgcttgat tttcacaaat
     1921 ttgtcgcgca cccggttggc gggattgtgg ggtagggacc ggccgatatt accgccccga
     1981 aatctaatca cgtcgcccct ggttttagga agctacgtct gactggatta acgccgcaat
     2041 ctggtttttc accatagcag aaagctctcc actgcggcac cctaacgatc aacttcccaa
     2101 tcattgaatc aggacaacta aggagcgtcg gatgtaagca agcctgcgac tactgggatt
     2161 aatttaccca gcgagcgtcc acctttcctt cccggcgcga agaaacaaga ttccgctgcg
     2221 ccatgcctac gcaggcagaa aaccatgccg cgttggcagg aatgtgcaga ttgaaatcaa
     2281 tgaagctatg aacgagaagg ccgcaaactg cgagcgtcgc ccccaattgc atccatcccg
     2341 agtgttcaag gatcctctta tccaaattct ttaacgttag acgaaagaac aggagtaa
//
LOCUS       DASYVO010000202         1669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_229281, whole genome shotgun sequence.
ACCESSION   DASYVO010000202 DASYVO010000000
VERSION     DASYVO010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229281"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1669)
                     /locus_tag="VGR03_06840"
     CDS             complement(<1..>1669)
                     /locus_tag="VGR03_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994026.1"
                     /translation="DSNVIYVGSGEADMRASISYGNGMYKSTDGGKTWLHIGLEDSRQ
                     IGRILVDPRDANKVFVAALGHAYGPNQERGLFRSKDGGKSWQKILFHDENTGGIDLAF
                     EPGNPKMIYASLWQTRRPPWSIYAPSNGPGSGLFRSTDGGDHWERVSGQGLPSEGLGR
                     IGIAFAPSNPRRIYLIVDAKEGGLYRSDNSGQSWQRVSNDRRIWQRGWYFGEVSVDPK
                     DPDTVYVPNTTTYQSRDGGKTFAAFKGAPGGDDYHELWIDPDEPKRMILSSDQGAIVT
                     RNGGETWSSWFNQPTAQFYHVATDNQFPYWVYGAQQDSGAAATPSRSRSRSLNFHDWR
                     PMDAGDENGYIAPDPLNPGVSFGGFVARQDFGNEQLQQLPPTLAHPGDYRRTWTLPLV
                     FSPIDPHVLYFGSQVLFRTADGGSSWQVISPDITRDDPGVPPSLDAATAADAPKGKRH
                     GVIYTIGPSPVRAGEIWAGTDDGLIQLTQDEGKTWENVTPAELTPWSKVTHIEASHFD
                     AGTAYAAVDRHRLEDYQAYLYRTRDFGKTWQRVSNGIPDGSFLNCVRE"
BASE COUNT          318 a          538 c          453 g          360 t
ORIGIN      
        1 cttcccgcac gcagttcagg aaacttccgt caggaattcc atttgagacg cgttgccagg
       61 ttttgccgaa atcgcgcgtg cgatagagat acgcctgata gtcctccagg cgatgcctgt
      121 ccaccgcggc atacgccgtc cccgcatcga aatgcgaagc ttcaatgtgt gtcaccttgc
      181 tccaaggcgt cagttccgcc ggcgtaacat tctcccacgt ttttccctcg tcctgcgtca
      241 gttgaatcag gccgtcatcc gtgcccgccc agatttcccc agcccgcacg ggcgatggcc
      301 caatcgtgta aatcacgccg tggcgtttgc cctttggagc atccgcggct gtcgctgcgt
      361 ccagactcgg cggcactccc ggatcatcgc gcgtgatatc aggactgatt acttgccagg
      421 agctgccgcc atccgccgtt cgaaacagaa cttgtgagcc gaagtacagc acatggggat
      481 cgattggcga aaaaactagc ggcagcgtcc acgtgcgacg gtaatcgccg ggatgcgcca
      541 gcgtcggcgg tagttgctgg agctgttcgt tgccgaaatc ctgccgcgcg acaaagccgc
      601 cgaacgacac cccgggattc agcgggtccg gcgcaatgta gccattttcg tcgccggcgt
      661 ccatcggccg ccaatcatga aaattcaacg aacgagacct gctgcggctg ggagtagctg
      721 ccgcaccgct gtcctgctgc gcgccgtaca cccagtaagg aaactgatta tccgtcgcca
      781 cgtgataaaa ctgcgccgtt ggctggttga accaggaact ccaagtctca ccgccgttgc
      841 gcgtcacgat cgcaccctgg tcgctgctga gaatcatccg cttcggctcg tcgggatcaa
      901 tccaaagttc gtggtagtcg tcgccccccg gcgcgccttt aaacgccgcg aacgtcttcc
      961 ctccgtcgcg cgactggtag gttgtggtgt tcgggacgta cacggtgtcg ggatctttgg
     1021 gatcaacgct gacttccccg aagtaccagc cgcgctgcca gatgcgccgg tcattcgaaa
     1081 cgcgctgcca actctgcccg ctgttgtcgg aacgatagag cccgccttcc ttggcgtcca
     1141 cgatcaaata aatccgccgc ggattgctcg gcgcaaaagc aatcccgatc cgtccgagac
     1201 cttccgaagg caatccctgc ccgctcacgc gttcccaatg atcgccgcca tccgtcgagc
     1261 ggaagagtcc gcttcctggc ccattcgaag gggcgtagat gctccacggt ggccttcgtg
     1321 tttgccacag cgatgcgtaa atcatttttg gattgccggg ttcgaacgct agatcgatcc
     1381 cgccggtgtt ttcatcatga aacagaattt tctgccagct ttttcctcca tctttcgaac
     1441 ggaagaggcc tcgctcctgg ttcggcccgt aagcatgacc tagcgcggca acaaaaactt
     1501 tgtttgcatc tcgcgggtcc acgaggatcc gcccgatctg ccgcgaatcc tccagcccga
     1561 tgtgcaacca cgtctttccg ccgtccgtgg acttgtacat cccattcccg taggaaattg
     1621 aggcgcgcat gtcggcttcg ccggagccca cgtaaattac gtttgaatc
//
LOCUS       DASYVO010000203         3119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_273328, whole genome shotgun sequence.
ACCESSION   DASYVO010000203 DASYVO010000000
VERSION     DASYVO010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273328"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..643
                     /locus_tag="VGR03_06845"
     CDS             56..643
                     /locus_tag="VGR03_06845"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1994027.1"
                     /translation="MKPIIYVRHGSTPYDSDVEKIQSWDDSQPVTAAGKRESVRVAQR
                     LSKLPIAAIVSSHLSRAKTTADAIGKAAGVPVETTDKLAPWKKGKFTGKNVKDVKGEL
                     DAYQNDKPDEKIPGGESFNDYLGRLKAGVEHVRGLQRANPDGHVVAVTHSTDLLALPH
                     IHSAGEEDIPTSGAPKPGSAVTVKTRQGKTSLEPI"
     gene            674..1558
                     /locus_tag="VGR03_06850"
     CDS             674..1558
                     /locus_tag="VGR03_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994028.1"
                     /translation="MDELNLNAPAVDPGAGADPGVGAPAGGADPGAGAGTPGAGAPAA
                     AAVESANAKQFREAYEALKTKYEPWEKLGAKPDEVGASHTTIQKISAEARELGEKLGF
                     KAEQISQALKDDLAGTLIWLREETKRREDAAGGPDVREVVKRQLEEGLKPFKEQMTRQ
                     QEAEATRKSDEGNFYFNQEFKKQLEAKYPDEATRPGPLGQGKIYQMACDLMMKDKEAL
                     DGLMQGKVSSVAKYFEQANADYLALVGEHGTRENVRVQRSPGERPATPPNGKRRATLE
                     ELVEEPGHINKKYALPQR"
     gene            1610..1780
                     /locus_tag="VGR03_06855"
     CDS             1610..1780
                     /locus_tag="VGR03_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994029.1"
                     /translation="MKEMDVVEINGRIGTIVDLHKGVAVVQFPNFYFEGFAAEQLKVV
                     WTARAEQVSPAN"
     gene            1941..>3119
                     /locus_tag="VGR03_06860"
     CDS             1941..>3119
                     /locus_tag="VGR03_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994030.1"
                     /translation="MRVNTKVVLDMVSNEIIEREFYEYDGPVALCAVDTSTFLADMKY
                     VYGAMTDQISTLPAVMNLFGDGSKVGKPINNIGVRGFVFGARMAPNWNMGFRKEGVTG
                     VGNAGNQGLANSTVLLKYAYDPQLITGQAENLSKGEARAFMQAKALTSKYDLLDMVSH
                     ANVLVCGADRGGQLGTVNASAAGSITVPALSPTSAAALYIRVGQPIDCAPVGGGALSL
                     SGAVISAINYATGVITHNGGTATVGHAIYLSGEAPVVAGDFPLTCEGLISLVSDTGAV
                     QGLNPATAGQQAWAAYAFDNANAQITSQAIQQLRQFTKNRGGVDPDIFIFPSAQINQL
                     VAIATSTIRFDLNAANAKGNAAKRAVDLGFNVFEYAGLQCLEDKDARPDRIFAIAIET
                     M"
BASE COUNT          741 a          786 c         1052 g          540 t
ORIGIN      
        1 ggattcaaga ttccggattc cggattccga aaaggaaagg gttgaagggc aaggaatgaa
       61 accgatcatc tatgtgcgcc acgggagcac gccatatgat tcggacgtgg agaaaatcca
      121 gagttgggat gactcgcagc cggttaccgc ggctggaaag cgagagagtg tacgcgttgc
      181 tcagagactg tcgaaactac caatcgcggc catcgtcagc tcgcaccttt cgcgtgccaa
      241 aaccactgca gacgcgattg gcaaggcggc tggcgttccg gtggagacta cggacaagct
      301 agctccgtgg aagaaaggga aatttaccgg gaagaacgtt aaggacgtga agggcgagct
      361 ggatgcgtac cagaacgaca agccggacga aaaaatcccc ggtggggaat cgttcaacga
      421 ttatctgggg cggctcaagg cgggcgtgga gcatgtacgc ggattgcagc gcgcgaaccc
      481 cgacggtcat gtcgtggccg tcacgcattc aaccgatttg ctggcgctac ctcacatcca
      541 ctcggccggc gaagaagaca tcccgaccag cggtgcacca aaaccgggca gtgcggtgac
      601 ggtgaagacg cggcagggga agacgagttt ggagccgatt taggattccg gattccggaa
      661 agggaagtga acgatggacg aacttaattt gaacgcgccg gcggttgatc caggagcggg
      721 ggcggatccg ggagttggtg cgccggcggg tggcgcggat ccgggagcgg gtgcgggaac
      781 tccgggggcg ggagctcctg ctgctgcggc ggtggaaagt gcgaacgcga aacagtttcg
      841 cgaggcgtac gaagcgctga agaccaagta cgagccgtgg gaaaaacttg gcgccaagcc
      901 ggatgaagtg ggggcgagtc acacaaccat tcagaagatt tcggctgagg cgcgcgagct
      961 gggggagaag ctgggattta aggcggagca gatcagccag gcgctcaagg atgacctggc
     1021 cgggacgctg atttggttgc gcgaggaaac gaagcgtcgc gaggatgcgg cgggcgggcc
     1081 ggacgttcgt gaagtggtga agcggcagct cgaggaaggg ctgaaaccgt ttaaagagca
     1141 gatgacgcgg cagcaggagg cggaggccac tcgcaaatcg gacgaaggga atttttattt
     1201 caaccaggag tttaagaagc agctcgaagc gaagtatccg gacgaggcca cacggccggg
     1261 accgctgggg caggggaaaa tctatcagat ggcctgcgac ctgatgatga aggacaagga
     1321 agcgctcgac gggctgatgc aaggaaaagt ttcaagcgtg gcgaaatatt ttgagcaggc
     1381 gaacgcggac tatcttgccc tggtgggcga gcatgggacg cgggagaacg tgagggtgca
     1441 gcgttcgcct ggggagagac cagccacgcc gccgaatggg aagaggcggg cgacgctcga
     1501 ggaattagtg gaagagccgg gccacatcaa caagaagtac gcgttgccgc aacggtaggc
     1561 tggaaatacc ggattcaaga ttccggattc cggactcgga cgtaaagacg tgaaggaaat
     1621 ggacgtggtt gaaattaacg gccggattgg gaccatcgtc gacctccaca agggcgtcgc
     1681 ggtggtgcag ttccctaatt tctattttga aggttttgca gcagagcaac tgaaggtagt
     1741 ttggaccgca agggcagagc aggttagtcc cgccaactaa aaagattcca gattcaggat
     1801 tctgaatccc ggaacgaccg cacgacgtaa caccgcaagg gcagagcagg gctccccgcc
     1861 agagcgaacg ggctgaattt agcaacggtt cgccctcgtt gaacgaagac cacaaattca
     1921 atcaacgagg agtgtgcccc atgcgggtca acacgaaagt ggttttggac atggtctcga
     1981 acgagatcat cgaacgcgag ttttacgaat acgacgggcc ggtggccttg tgcgccgtgg
     2041 acaccagcac gtttctggct gatatgaagt acgtctacgg cgcgatgact gaccagattt
     2101 cgaccctgcc ggcagtaatg aatctgtttg gcgacggctc gaaagtaggg aaaccgatca
     2161 acaacattgg tgtgcggggc ttcgtgtttg gcgcccgcat ggcgcccaac tggaacatgg
     2221 gtttcaggaa agagggcgtg acgggcgtgg gcaatgcggg caaccagggc ctggcaaact
     2281 cgaccgtgct gctgaaatac gcctatgacc ctcaactgat taccgggcag gcggaaaacc
     2341 tgtcgaaggg cgaggcgcga gccttcatgc aagcgaaggc gttgacttcg aagtacgacc
     2401 tgctggacat ggtgtcacac gcgaacgtgc tggtgtgtgg cgcggatcgc ggcgggcagc
     2461 ttggaacggt gaacgcatcg gccgcgggct cgatcacggt gccagcgctt tcaccgactt
     2521 cggcggctgc gctctatatc cgcgtcggac aaccgattga ttgcgctccg gtgggtggcg
     2581 gcgcgctgtc actttcgggc gccgtaatct cggcaatcaa ctacgcgacg ggagtgatca
     2641 cacacaacgg cggaacggct acggttggtc acgctatcta tctctcgggt gaggcgccgg
     2701 tggtagcggg cgactttcca ctgacctgcg aaggcctgat ctcgctcgtc tcggacacag
     2761 gcgcggtgca agggctgaac ccggctactg ctggacagca ggcatgggcg gcttacgcgt
     2821 tcgacaatgc caacgcgcag atcacctcgc aggcgatcca acagcttcgg caattcacca
     2881 aaaaccgggg cggcgtcgat ccggacatct ttatttttcc ttcggcgcaa atcaaccaac
     2941 tggtggcgat tgccacatca accattcgct tcgatttaaa cgcggcgaat gcgaagggca
     3001 acgcggccaa gcgggcggtg gatcttggct tcaacgtatt cgagtacgcc ggcttacagt
     3061 gtctcgagga taaagacgcg cggccggaca gaatcttcgc catagccatc gagacgatg
//
LOCUS       DASYVO010000204         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_273343, whole genome shotgun sequence.
ACCESSION   DASYVO010000204 DASYVO010000000
VERSION     DASYVO010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273343"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VGR03_06865"
     CDS             complement(<1..304)
                     /locus_tag="VGR03_06865"
                     /inference="COORDINATES: protein motif:HMM:NF027503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HEV1994031.1"
                     /translation="MAKAREKAKARTNASKGKQKLTEAHIAVHWKEEAYFHPSKEFIA
                     QANLSDPAFVKKFSEKHFPECFDHYASLLHWDKPWRKTLDTSHPPFWKWFVGGKLNA"
     gene            514..1827
                     /locus_tag="VGR03_06870"
     CDS             514..1827
                     /locus_tag="VGR03_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1994032.1"
                     /translation="MSTAGIHPEAPPAKATPLTRNQVRGFWASWGGWTLDGMDSFIYA
                     LVLLPSLRDLLPRSGIAATKGNLLYYGGLLFALFLFGWGLAFLWGPVGDKFGRVRTLM
                     LTIVWYSVFTFLSALVMNVWQLAVMRLLAGIGIGGEWAMGGTFVAEEWPEDRRRAGAG
                     YMHTGYYVGVFLAALLNYWIGSRYGWRAMFAVGGLPALLLAWVRHGVTEPERWKQKEG
                     VVRKWQIWQPLATLFSSGMRRRTILNSLLMLASICGLWAGTVYVPAAVTVLAEAAGRA
                     GPSAAQLASRATMLVSFATILGCLAMPWLAEKLGRRGALGFFFALMLVFIALTFGKVF
                     YLGAAALPWFFVCLFFLGFGGANFAVYTLWLPEQYPTECRASAFAFSTSFARFGGAGI
                     TFLVGAGVQHYGSLGTPVALTSIAFAIGLLLIPFGVETRGQTLPA"
     gene            1852..>2079
                     /locus_tag="VGR03_06875"
     CDS             1852..>2079
                     /locus_tag="VGR03_06875"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEV1994033.1"
                     /translation="MTEVENKVFSIEALREFCSRVFLHFGVPKNDAMQAAEVLACADL
                     RGIDSHGVARLHSYFDMLSEGRINPKPQIKVV"
BASE COUNT          349 a          544 c          665 g          521 t
ORIGIN      
        1 aggcattgag tttgccgccg acgaaccatt tccagaaggg aggatggctg gtgtcgagcg
       61 tcttgcgcca gggcttgtcc caatggagga gggaagcgta atggtcgaag cattccggaa
      121 aatgtttttc gctgaatttc ttgacgaacg caggatcgga aagattcgcc tgggcgatga
      181 attccttgga aggatggaaa tacgcttctt ccttccagtg gacggcgatg tgcgcttcgg
      241 tgagcttttg cttgcccttc gatgcgttcg ttcttgcttt cgctttttcg cgggctttgg
      301 ccacggcggc aactcccctt ggaagatcga tttcggttgt gacgctgcaa cgagggacaa
      361 aatgatgcta ccacgcgggt gcatgagtca atcggctaga gcgtgaaccc ggaactgtga
      421 tggaaggcgc cattgacaaa ggctgctgtg gggcgtttca aacacttcga tttttctttt
      481 ccccgcaaca gcttttgata tagtccgcgc tgtatgtcca ccgctggtat ccatccggaa
      541 gcaccacccg ccaaagccac gccactcacg cgaaatcagg ttcggggttt ctgggcctcg
      601 tggggcgggt ggacgctcga cggcatggat tcgttcattt atgcccttgt gcttttgcct
      661 tctctgcgcg atctgctccc gcgttccggc attgccgcaa cgaaaggcaa cctcctgtat
      721 tacggcggac tgctattcgc gctttttcta tttggatggg ggctggcatt tctgtggggc
      781 ccggtcggcg ataagtttgg gcgggttcgg actttgatgc tgacaatcgt ctggtactcg
      841 gtgttcactt ttctcagtgc gctggtgatg aatgtctggc agcttgcggt gatgcgcttg
      901 ctggcaggaa tcggaatcgg tggcgaatgg gccatgggcg gcacgttcgt tgccgaggag
      961 tggccggaag accgccggcg cgccggcgcg ggatacatgc acacgggata ctacgtggga
     1021 gtttttctgg ccgcgctgct gaactactgg atcggcagcc gctatggatg gcgcgcgatg
     1081 ttcgcggtcg gcgggttgcc ggcgcttctg ctggcttggg tgcgtcacgg cgtgaccgag
     1141 ccagagcgtt ggaagcagaa agaaggggtc gtccgaaagt ggcagatctg gcaaccgctc
     1201 gcgacacttt tttccagtgg aatgcgccgc agaacgatac tgaattcctt gcttatgctg
     1261 gcttcgatat gcggcctctg ggcgggaacg gtgtacgttc ctgctgccgt gacggtgctt
     1321 gcggaagcag cggggcgggc cggaccatcg gcggcgcagc tcgcttcgcg ggcgaccatg
     1381 ctagtctctt ttgcgacgat tctcggatgc ctggcgatgc cctggctcgc ggaaaaactg
     1441 ggccggcgcg gcgccttggg gttttttttc gcgttgatgc tggtcttcat cgcgctcacg
     1501 tttggaaaag ttttctatct cggggcggcg gcgctcccat ggttttttgt ttgccttttc
     1561 tttctcggtt ttggcggcgc gaattttgcg gtttatacgc tgtggctgcc ggaacaatat
     1621 ccgacggagt gccgggcgag cgctttcgcc ttctcaactt cgtttgcgcg ttttggcggt
     1681 gcaggaatca cgtttctggt cggcgcgggg gtgcagcatt acggatcgct gggaactccc
     1741 gtcgcgctga cgtccatcgc cttcgccatt ggactactgc tcattccttt tggagttgag
     1801 acccggggcc aaaccctgcc cgcgtagatt tcgcgatggc cgaggatgaa catgaccgaa
     1861 gttgagaaca aagttttctc catagaagcc ttgcgtgaat tctgcagtcg cgtgttcctg
     1921 cacttcggag tccccaagaa cgatgcgatg caggcggccg aggtgctcgc ttgcgcggac
     1981 ttgcgcggca tcgattcgca cggtgtggcc cggctgcaca gttatttcga catgctcagc
     2041 gaggggcgaa tcaatcccaa gccccaaatc aaagtggtg
//
LOCUS       DASYVO010000205         8124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_19146, whole genome shotgun sequence.
ACCESSION   DASYVO010000205 DASYVO010000000
VERSION     DASYVO010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8124
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19146"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VGR03_06880"
     CDS             <1..531
                     /locus_tag="VGR03_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEV1994034.1"
                     /translation="VLLGASAALPHGTVQPQKLKEGDVVLIDGGTAVDSYQSDVTRTG
                     IFGKQSEKVARVYEIVRKAQDAALDAARAGRLSGTVDDAARKVIANAGYGPDYKYFTH
                     RLGHGMGLDGHEHPYLVRGSKTVLEPGMTFSNEPGIYIPGEFGMRCEDDMVIAADGPA
                     RLLTPSFAVSLEKPLG"
     gene            complement(835..1830)
                     /locus_tag="VGR03_06885"
     CDS             complement(835..1830)
                     /locus_tag="VGR03_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV1994035.1"
                     /translation="MTTILPEFQPHPLLKNGHAMTIASAFVPRRFDIPPAEARLFQVD
                     PESRLLAHCHWQPGKRKVAPVIVIVHGLEGSSDGNYVHGIAEKAFHRGFHVLRMNQRN
                     CGGTEALTPTLYNSGMSGDYRAVFEELSNADGFEQIFFAGYSMGGNLVTKMAGEFGAA
                     VPKALRGVCAVCPALDLAACADALERADNYFYQRRFVNGLMSRYARKHKMLPERYPIA
                     GLRPVRSVRQFDDVITAPQFGYRNAQDYYDNVGAKRVAAQIRVPMLVITAQDDPFVPY
                     ASFLAAKINQNPAIQFVAPQHGGHCGFISKYSGSERFWAEQRIVEFCAALSGNGL"
     gene            1969..2409
                     /locus_tag="VGR03_06890"
     CDS             1969..2409
                     /locus_tag="VGR03_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEV1994036.1"
                     /translation="MKGRVEDRAMKFAECYYRKKGWEVKNVARARGEHFGYDLFLERG
                     SERLKVEVKGSAKPYHGIPDLYKNEVDEHKRLRADFLCVVYFPLGKPEKLAIIPRDRI
                     LPDCLKPKISYCISSKFKGPESIREFLVDTEETCSPQKRRAIES"
     gene            2632..4092
                     /gene="guaB"
                     /locus_tag="VGR03_06895"
     CDS             2632..4092
                     /gene="guaB"
                     /locus_tag="VGR03_06895"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV1994037.1"
                     /translation="MMDGPITEGLTFDDVLLLPGKSAVLPTEVDTATNFSRKLTINIP
                     LASAAMDTVTESRLAIAIARQGGIGMVHRNMPIDRQGEEVDRVKRSESGMIVDPVTIS
                     PDITLRQALEIMNKYKISGLPVTRGSRLTGILTNRDLRFEKNHAQLVGSVMTKENLVT
                     VPVGTTLEEAERILQKHRIEKLLVVDQDFNLKGLITVKDIQKKLEYPRAAKDEHGRLR
                     VGAAVGATGDFLERAVEMSKTKVDVLAIDTAHGHSERVMEAIKAIRHRLPEMQLVAGN
                     VATYEGAKELISLGIDGLKVGIGPGSICTTRVVTGAGVPQLTAIMEAARAAKDTDVAI
                     IADGGIKYSGDISKALAAGASCVMIGSLFAGTEESPGETILYQGRTFKSYRGMGSTGA
                     MEAGSADRYAQEGAERGKSVPEGVEGRVPYKGSLGALTDQLVGGLKSGMGYVGAATLK
                     EFRERGRFIRITAAGLRESHVHDVIITKEAPNYRME"
     gene            4155..4712
                     /locus_tag="VGR03_06900"
     CDS             4155..4712
                     /locus_tag="VGR03_06900"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1994038.1"
                     /translation="MPELQPWRNPRPAPQLEEIRSRMRPPRALVWIRAWWPALLWAGV
                     IFSASTDAFSSEHTAPVFEPILRWIVRSLTDAQFLMIHYFLRKSAHFTEYFVFCLLLY
                     RGVRGDRKGWRWTWGVAALFCAAGYSALDEIHQAFVASRTASAYDSLLDSIGAFAAFG
                     VLWMWFSWRRSAREAEIVASKAPSV"
     gene            complement(4817..5236)
                     /gene="rplQ"
                     /locus_tag="VGR03_06905"
     CDS             complement(4817..5236)
                     /gene="rplQ"
                     /locus_tag="VGR03_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV1994039.1"
                     /translation="MRHLNSGSKLGKQPAHRRAVLRSLVTNLFEHGRITTTLTRAKAA
                     RPVAEKMITLGKRDTLQSRRQAASYIMLPAVTQKLFSEIAPKFADRAGGYTRIIHAGN
                     RVGDNAKVAILELIGYEFKLKEKKEKTKKEEPAEAKS"
     gene            complement(5260..6288)
                     /locus_tag="VGR03_06910"
     CDS             complement(5260..6288)
                     /locus_tag="VGR03_06910"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV1994040.1"
                     /translation="MWKGFQKPKRLASELESLTEKYGRFSAQPFERGWGTTVGNALRR
                     ALLSSIEGAAITAVKIEGVLHEFSSIPGVVEDATDIILNLKQVPIKLNTDLPKTIYVN
                     LEQAGPVTSAQIEEDADFAVLDKSVYIATVSEGGKLQIEMRVKNGRGYVAADRNYDED
                     LPIGYIPVDSVHSPVRKVNYAVEAARLGQMTDYEKLMIEVWTNGAITPQDSIGLAAKL
                     VKDHMSIFINFEEPVDTVEVPQDIAHDPRLEHLDRSVEELELSVRSYNCLKNANIQTI
                     RELVQKSENEMLKTKNFGRKSLNEIKDILVKMGLALGMKFDEHGRIIWPPLPSQTAAP
                     ASEENVGL"
     gene            complement(6397..7029)
                     /gene="rpsD"
                     /locus_tag="VGR03_06915"
     CDS             complement(6397..7029)
                     /gene="rpsD"
                     /locus_tag="VGR03_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV1994041.1"
                     /translation="MARHRDAVCRLCRREGQKLFLKGLRCFTEKCAIEKRNFVPGQHG
                     QSRRPKVAGYGLQLREKQKVKRTYGLLERQFRNYFEKAERAKGPTGENLIIMLERRLD
                     NVVWRAGLASSRSQARQLVLHGHVRINGKKVNIPSYLVNVGEEVAIKEKMHQNAMVLE
                     ARNVAQSQNAVPWLEQEREQFKAKVVAMPKRDDVQTPPINEQLIVELYSK"
     gene            complement(7083..7538)
                     /gene="rpsK"
                     /locus_tag="VGR03_06920"
     CDS             complement(7083..7538)
                     /gene="rpsK"
                     /locus_tag="VGR03_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV1994042.1"
                     /translation="MAKEPKAAAPDAGAPGKPAAAAKRKKEFKKKRERRIIPHGVVYV
                     AASFNNTQISITDTDGRVVCWSSAGAIGFKGSRKGTPYAAQQAGLAAAGVARDQYGMK
                     SVEVRVKGPGSGRESAVRALAAAGLHIVLIRDVTPIPHNGCRPPKRRRV"
     gene            complement(7641..8030)
                     /gene="rpsM"
                     /locus_tag="VGR03_06925"
     CDS             complement(7641..8030)
                     /gene="rpsM"
                     /locus_tag="VGR03_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV1994043.1"
                     /translation="MARIAGVDLPPQKRLWVGLTSIYGIGHERAKKLAAKAGVDHQKK
                     IRDLTEDEINHLRQEIEKEGRVEGDLRKEIQMNIRRLIEIQAYRGIRHRRNLPVRGQR
                     THTNARTRKGPRRGAVAAKKKVLGKKG"
     gene            complement(8053..>8124)
                     /gene="rpmJ"
                     /locus_tag="VGR03_06930"
     CDS             complement(8053..>8124)
                     /gene="rpmJ"
                     /locus_tag="VGR03_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV1994044.1"
                     /translation="KVIHRRGVVRVICINPKHKQRQG"
BASE COUNT         1582 a         2279 c         2527 g         1736 t
ORIGIN      
        1 gttttgctgg gcgcttcggc cgcgttgccg cacggcacgg tgcagccaca aaaactgaaa
       61 gagggcgatg tcgtgctgat tgacggcggc acggcagtgg atagctatca atcggacgtt
      121 actcggacgg ggatcttcgg caagcagtcg gaaaaagtgg cgcgggttta cgaaatcgtg
      181 cgcaaggcgc aggatgcggc tctcgatgcg gctcgtgctg gacggctatc gggcacagtg
      241 gatgatgccg cgcgcaaggt gatcgccaac gccgggtatg gaccggacta caagtatttc
      301 acgcaccgtc tcggccatgg catggggctc gacggacacg agcatccgta tcttgtgcgc
      361 ggaagcaaga ccgtgcttga gccggggatg acattttcga atgagccggg gatctatatt
      421 cccggtgaat ttggaatgcg ctgcgaagac gacatggtta ttgcggcgga cgggccggcg
      481 cgacttttga cgccgagttt tgcggtttcg ttggaaaaac cgctgggata aaaacatgtt
      541 gttagttgtt agtttttagt taagagtttt cggaaaatcg agactctagc ggatttctaa
      601 aaggtattcc gggatgggga accaaaaaga agaaaagcct cttgaggctg gatttggttg
      661 ccaagctggg gcgcagcatg ctgcgcccct acaaacgagc aacgatccgc gggtttgttg
      721 tgtgtttcgc gtagggcatc gcagttcccc ttcacgcgtg gtgggaaatg cggactgggc
      781 gtttgggatc ctcgctgaac gtttacgcgc agaaatcttt gaatgtgtgc cttattacag
      841 gccattaccg ctgagcgcgg cgcagaattc gacgatgcgc tgctcggccc aaaagcgttc
      901 gcttccggag tatttcgaaa tgaagccgca atggccgcca tgttggggcg cgacaaactg
      961 gatggcggga ttctggttga tcttggcggc caggaaggaa gcgtagggga cgaagggatc
     1021 atcctgcgcg gtgatcacca gcatgggcac gcggatctga gccgcaacgc gtttcgcgcc
     1081 aacgttatcg taatagtctt gggcgttgcg gtagccgaac tgcggcgcgg tgatgacgtc
     1141 atcgaattga cggaccgatc ggaccggtcg caggcccgcg atcgggtagc gctccggaag
     1201 cattttgtgt tttctcgcgt agcgggacat caggccattc acaaagcgcc gctggtaaaa
     1261 atagttgtcc gcgcgctcga gcgcatcggc gcaagccgcg aggtccaggg cggggcagac
     1321 ggcgcagaca ccacgcaacg ccttcggcac ggccgcacca aactctccgg ccatcttggt
     1381 caccaggttt ccgcccatcg agtagccggc aaagaagatc tgttcgaaac cgtccgcatt
     1441 tgatagctct tcgaagacgg cccggtagtc cccgctcata ccggagttgt acaaggtggg
     1501 cgtcaaagct tccgtgccgc cgcaattccg ctggttcatg cgcaggacgt gaaaaccgcg
     1561 gtggaaagcc ttctccgcaa tgccgtgcac gtaattcccg tcgctggaac cctcgaggcc
     1621 atgaacgatg acgatgaccg gagcgacctt gcgcttcccg ggttgccagt ggcaatgtgc
     1681 gagcaagcgg gattccggat cgacctgaaa cagcctggct tcggcgggcg gaatatcgaa
     1741 gcgcctgggc acgaaagccg aggcgatggt cattgcgtgt ccgtttttca gaagcgggtg
     1801 cggctggaat tcaggcaaga tggttgtcac gctcaaactc tatttcacgc cccgctcgta
     1861 ggcaagcatc gctattgtag cagggcgtgg tatctgaaat cggccttgaa gcttagcgac
     1921 gagtgcgctg tacgtataga atcttttgat cttggtctgg aggacgaaat gaaaggtcgc
     1981 gtggaagatc gcgcaatgaa gttcgcggag tgttactaca ggaaaaaggg ctgggaagta
     2041 aaaaacgtgg ctcgcgctcg cggggaacac tttggatacg acctgttcct tgagagaggt
     2101 tccgaacggc tgaaggttga ggtcaagggc agcgccaaac cataccacgg gattcccgat
     2161 ctctacaaaa atgaagtcga tgaacacaag aggctaagag cagacttctt gtgtgttgtg
     2221 tatttcccgc taggcaagcc ggaaaagttg gcaattatcc cccgtgatcg cattcttcca
     2281 gattgtctta aaccaaagat ctcctattgc ataagcagca aattcaaagg cccggagtcg
     2341 atccgcgagt ttttggtcga tacggaggag acgtgctcac ctcagaagcg cagagcaatt
     2401 gagagttgat ttttcgcgtc gggatgctca ggaacgtcaa cgcagattcc agacaccacc
     2461 cagcggagag cagatttgac gcaaattccg caggacccta taatgggtgg ttcacggtga
     2521 tggatttttt caatttgatg agattaactg ccagcccagc ggggagccgc tgggctgcgc
     2581 tgctttaggg cagaaaacag gcggaacaaa tttttcttac gtgaggctgg aatgatggac
     2641 ggtccgatta ccgaggggct tacctttgac gatgtgctgc tgctgccggg caaatcggcg
     2701 gtgttgccga cggaagtgga tacggcgacg aacttctcgc gcaagctgac gatcaacatt
     2761 ccgctggcgt ccgcggcgat ggacacagtg acggaatcgc gcctggcgat cgccatcgcg
     2821 aggcagggcg gcattggaat ggtgcaccgc aacatgccga tcgaccggca gggcgaggaa
     2881 gtggaccgcg tgaagcgctc ggaaagcgga atgatcgttg acccggtgac gatttctccg
     2941 gacatcacgc tgcggcaggc gctggaaatc atgaataagt acaagatttc cggcctgccg
     3001 gtgacgcgcg gctcgcggct tacaggaatt ctgaccaacc gcgacctgcg attcgagaaa
     3061 aatcatgcgc aactggtcgg ctcggtgatg acgaaagaaa atctggtgac cgtgccagtg
     3121 ggcacgacgc tggaagaggc cgagcgcatc ctgcaaaaac accgcatcga gaaacttctg
     3181 gtcgtcgatc aggatttcaa cctgaagggc ttgatcaccg tcaaagacat ccagaaaaaa
     3241 ctggaatatc cccgcgcggc gaaagatgag cacggccgat tgcgcgtggg cgccgccgtg
     3301 ggtgcgacgg gcgattttct ggagcgggcc gtggaaatgt cgaagacaaa agtcgacgtg
     3361 ctggccatcg atacggcgca cgggcactcc gagcgagtga tggaagcgat taaggcgatt
     3421 cggcatcgat tgccggaaat gcagctggtg gccggcaacg tggccactta cgagggcgcg
     3481 aaagaactga tttcgctggg catcgatggg ctgaaagtgg gcatcggacc gggctctatc
     3541 tgcacgacgc gcgtggtcac cggggcaggg gtgccgcaac tgacggccat aatggaagcc
     3601 gcgagggccg cgaaggacac ggacgtggcc attattgccg acggcggcat caagtattcg
     3661 ggcgacattt cgaaagcgct cgccgcgggc gcatcgtgcg tgatgattgg cagccttttt
     3721 gccggcacgg aagagtctcc cggagaaact attctctatc agggccgcac gttcaagtct
     3781 tatcgcggga tgggttcgac cggcgcgatg gaggcgggct ccgccgaccg ttacgcgcaa
     3841 gaaggcgcgg agcgcggcaa atccgttccg gaaggagtcg agggccgcgt gccctacaag
     3901 ggctctcttg gggcgctgac cgaccagctg gtcggcggac tcaagagtgg catgggttac
     3961 gtgggagcgg ccacgttgaa ggaattcaga gagaggggcc gattcattcg aatcaccgcc
     4021 gcgggattgc gcgaaagcca cgttcatgac gtgatcatca cgaaagaagc gccgaactat
     4081 cgtatggaat aagggcgcag ggaatagggg cgatggagta gcgtgaagaa agagatcgaa
     4141 ggagccaatt cttagtgccg gaactccagc cctggcgcaa cccgcgcccc gccccacagt
     4201 tggaagagat tcgaagccgc atgcggccgc cacgagcact ggtgtggata agagcctggt
     4261 ggcccgcttt gctgtgggca ggcgtcatct tctcggcgtc cacggacgcg ttttcgtcgg
     4321 agcataccgc cccggtgttc gagccgatcc tgcggtggat cgttcgctcg cttacggacg
     4381 ctcaattttt gatgatccac tatttcctgc gaaagagcgc gcacttcacg gaatattttg
     4441 tcttttgcct gttgctctat cgcggcgtgc gcggcgaccg caaagggtgg cgctggacgt
     4501 ggggtgttgc agcgctcttc tgcgcggcag gatattccgc gctagatgaa attcaccagg
     4561 cgttcgtcgc cagccgtacc gcttcggctt acgattccct gctcgactcg atcggcgctt
     4621 tcgccgcctt cggcgtgctg tggatgtggt tcagctggcg acgatccgca cgtgaagcgg
     4681 aaatagtggc aagcaaagct ccttctgtct gacgcgccat cgaacagcgg gcaattcaca
     4741 aaagagctgc gccaaacaaa aagcccgttc cggtttgccg gaacgggctg ctgaacgttg
     4801 gattgagaaa gggtgcttag ctcttcgctt cggcgggttc ttccttcttg gtcttttctt
     4861 tcttttcctt gagcttgaat tcgtagccga tgagctcgag gatagccact ttggcgttgt
     4921 cgccgacgcg gttcccggcg tggatgatgc gagtgtagcc gccggcgcgg tccgcgaatt
     4981 tcggcgcgat ctcgctgaac agcttctgcg tgacggcagg cagcatgatg taggaggccg
     5041 cctggcggcg cgattgcagc gtgtcccgtt tgccaagggt gatcattttc tccgccaccg
     5101 ggcgcgccgc tttggcgcgc gtcagcgtgg tagtgatccg cccgtgttca aacagattgg
     5161 tgaccaggct gcgcagcacc gcacggcgat gagccggctg ctttccgagt ttggaacctg
     5221 agttgagatg tcgcatcgct tatattgtcc cttgtcgctt tacagtccga cgttctcttc
     5281 tgatgccggt gcggcggttt ggcttgggag cggcggccaa atgatccggc cgtgctcgtc
     5341 gaacttcatg cccagcgcga ggcccatctt caccagtata tctttgatct cgttgagcga
     5401 cttgcggccg aagtttttgg tcttcagcat ctcgttctcg ctcttctgga cgagctcgcg
     5461 aatggtctgg atgttggcgt tcttcaggca attgtaggag cgcacggaaa gctcgagctc
     5521 ttcgacggag cggtccaggt gctcgaggcg cgggtcgtgg gcaatgtcct gcggcacctc
     5581 gacggtatcc acgggctctt cgaagttgat gaagatgctc atgtggtcct tgaccagctt
     5641 ggcggcgaga ccgatggagt cttgcggcgt gatcgcgccg ttggtccaca cttcgatcat
     5701 cagcttttcg tagtcggtca tctggccgag acgggcggct tcgacggcgt agttgacctt
     5761 gcgcaccggc gagtgaacgc tgtcgaccgg gatgtagccg atgggcagat cttcgtcata
     5821 gttgcggtcg gcggccacat agccgcggcc gttcttgacg cgcatctcga tctggagttt
     5881 gccgccttcg ctgaccgtgg cgatgtacac gctcttatcg aggacggcga agtccgcgtc
     5941 ttcctcgatt tgcgccgagg tgaccggacc ggcctgctcg aggttgacgt aaatcgtctt
     6001 cggcaggtcg gtgttgagct tgatgggcac ctgcttcagg ttgaggatga tgtccgtggc
     6061 atcctcgacc acgccgggga tcgaagaaaa ttcgtgcagc acgccttcga tcttcacggc
     6121 agtgatggca gcgccttcaa tcgaactgag cagcgcgcga cgcagcgcgt ttcctacggt
     6181 cgtgccccag ccgcgctcaa aaggctgcgc ggaaaaccgc ccgtacttct cggtgagcga
     6241 ttccagctcg gacgcaagac gcttcggctt ctgaaaaccc ttccacatcg tgttccttcc
     6301 ttctggggac cgattgaaac ggccccccca tgctcgtaac ctgcggcccg aatcggaccg
     6361 gaaagatttg actggcgacc cctagcggcg gggcggctac ttcgagtaca actcgacgat
     6421 gagctgctcg ttgatcggcg gcgtctgcac gtcgtcgcgc ttcggcatgg ccacaacctt
     6481 ggccttgaac tgttcgcgct cctgttcgag ccagggcaca gcgttctggc tttgcgccac
     6541 gttgcgcgct tccagcacca tggcgttctg gtgcattttc tccttgatgg cgacttcctc
     6601 gcccacgtta acgaggtagg acgggatgtt gaccttcttc ccgtttatgc ggacgtggcc
     6661 gtgcagcacg agctgccgcg cctgcgaacg cgatgacgcc agacccgcgc gccacaccac
     6721 gttatccaga cggcgctcga gcatgatgat gaggttctcg ccggtcgggc ccttggcgcg
     6781 ctcggccttc tcaaaataat tgcggaactg ccgctccagc agcccgtaag tgcgcttgac
     6841 cttctgcttc tcgcgcaatt gcaggccgta tccggcgacc ttcgggcgcc gtgactgccc
     6901 gtgctgcccc ggcacgaagt tgcgcttctc aatcgcgcac ttctccgtaa agcaacgcaa
     6961 gcccttcaaa aatagtttct gcccttcccg gcggcacagg cggcagactg catctcggtg
     7021 tcttgccaac ttctattctc ccttcccaaa actctctgaa tcttttctct cggtcataaa
     7081 tatcaaacgc ggcggcgctt gggcggacgg cagccgttgt gcggaatcgg cgtgacgtcg
     7141 cggatcagca cgatgtggag ccccgcggca gccaacgcgc gcacggcgga ctcgcgtccg
     7201 gaaccggggc ccttcacgcg gacttccacg ctcttcatgc cgtactggtc gcgcgccacg
     7261 cccgcggcag ccagcccggc ctgctgcgcg gcgtacggcg tgcccttgcg cgatcccttg
     7321 aagccgatcg cacccgcgga ggaccagcac acgactcggc catccgtgtc tgtgatggag
     7381 atctgcgtgt tgttgaacga cgcggcgacg tagaccacac cgtgcggaat gatgcggcgc
     7441 tcgcgcttct tcttgaattc tttcttgcgc ttcgctgccg ctgccggttt tcccggtgct
     7501 cctgcatctg gcgctgcggc ttttggttct tttgccacga ttcactccat tcctctgacg
     7561 gcttgaaaac tcttacgacc gcaaaagcgg cagcacggct gccgcactcc aaatggcgcc
     7621 tgcaaggcgc cgcgaaaaaa ctagcccttc ttgcccaaga ccttcttctt cgccgccacc
     7681 gcgccgcgcc gcggaccctt gcgggtgcgc gcgttggtgt gcgttcgctg gccgcgtacc
     7741 ggcaggttcc ggcggtgccg gatcccgcgg taagcctgaa tttcaatcag ccggcggatg
     7801 ttcatctgaa tctccttgcg gagatcgcct tccacacggc cttctttttc aatttcctgg
     7861 cgaagatggt tgatttcatc ttccgtcagg tcgcgaatct ttttctggtg atcgacgccc
     7921 gcctttgccg cgagcttctt cgcgcgttcg tgcccgatgc catagatcga ggtcagccca
     7981 acccaaaggc gcttctgcgg cggaagatct acacctgcga tacgtgccat gccattctcc
     8041 taacgaggat gcttatcctt gacgctgctt gtgcttcgga ttgatacaga tcacccgaac
     8101 cacgccgcgc cggtggatca cctt
//
LOCUS       DASYVO010000206         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_74576, whole genome shotgun sequence.
ACCESSION   DASYVO010000206 DASYVO010000000
VERSION     DASYVO010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74576"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2021
                     /locus_tag="VGR03_06935"
     CDS             <1..2021
                     /locus_tag="VGR03_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV1994045.1"
                     /translation="FIVLKITGQSFNLMTLGGLAAAVGLVIDDAIVVLENIVLHRDAG
                     QSRAQAIQTALRELTIPLIGSTVTPIVVFLPLISTTGVTGSFFRALAVTMTVSLFTSL
                     LLALTWTPTLSLYLVRRKDTAEASASSSAGGPDAAALLAAEEAHLSGFFGRIVDFYAR
                     TMKAVLKRPWVLAASSVVIVVLSIVCYLSLGSGLLPEMDEGGFILDYDTPPGSSLAES
                     DRILQHIEKILHSVPEVENTSRRTGLQLGLAAVTEANKGDFTARLKSDRSRAIDEIIS
                     DVRSQIEQTEAATKIEFVQVLQDMIGDLTSAPEPIVIKLFSQDAKLLGDTAPQVADAI
                     GKIQIGKAHPVVDVLNGIENTTSGPAVTYQVNPAVAARAGFTPEEIALDAAAILEGEP
                     AAPPVILNDRAYTIRVRFPEQSRSSLDRMSNTLVTSTTGRTATLGSLATIIAEPGQTE
                     IRRENLQRVVQVTARLEGTDLGSGIAAVQKAVNDLHLPASIRVEYGGLYQEQQKSFHD
                     LVMVLFLALLLLFVVLLFEFRTFSAPTAILASALLSTFGGFLALLVTRTTFNVASFMG
                     MIMVIGIVAKNGILLLDAEHHFRDLGISAEDAMIQAGRRRLRPIAMTALATIAGMLPL
                     AFAIGAGSQMLQPLAITVVGGILSSMVLSLIFTPAIHYYLQPRKTTEA"
BASE COUNT          433 a          692 c          577 g          495 t
ORIGIN      
        1 ccttcatcgt cctaaagatt accggccaga gctttaatct catgaccctc ggcggtcttg
       61 ccgcggcggt cggcctggtt attgacgatg ccatcgtcgt gcttgaaaac atcgtcttgc
      121 accgcgatgc gggccagagc cgcgctcaag ccatccagac tgctctgcgc gaattgacaa
      181 tccccctgat cggttcgacg gtcacgccga ttgtcgtttt tctgccactc atctccacga
      241 caggcgtcac cggcagcttc ttccgcgccc tcgctgtcac catgaccgtt tctctcttca
      301 cctcgctcct tcttgccctg acctggactc caacgctaag tctgtacttg gtgcgccgaa
      361 aagacactgc tgaagcctcg gcatcgtcct ccgcgggcgg tccggacgct gcggcccttc
      421 tcgccgccga agaagcgcac ctttccggct ttttcgggcg catcgtggac ttttatgcgc
      481 gaacgatgaa ggctgtgctg aagcgtccct gggttctggc cgcgagttct gttgtaatcg
      541 ttgttctttc aattgtctgc tacctatcct tgggcagcgg cctgctcccc gagatggatg
      601 aaggaggatt catcctggac tatgacactc cgccgggaag ctcgctggcg gaatccgatc
      661 gcatccttca acacatcgaa aaaattctgc attccgtgcc ggaagtcgaa aacacgtcgc
      721 gccgcaccgg cttacagctt ggccttgctg ccgttaccga agcaaacaaa ggtgacttta
      781 ccgccaggct taagagcgac cgtagccgcg ctatcgatga aatcatctcc gacgtcaggt
      841 cgcaaatcga acagacggaa gccgccacaa aaatagaatt cgtccaggta ttgcaagaca
      901 tgatcggcga cctcacaagc gcccccgagc ccatcgtaat caaactcttt tcgcaggatg
      961 ccaagcttct cggagataca gctccgcagg tcgcggatgc tattggcaaa atccaaattg
     1021 ggaaagctca cccggtcgtg gacgtcctaa acggcatcga aaacaccacc agcggtccgg
     1081 ccgttacgta tcaggtgaat cctgccgtcg ctgcgcgcgc cgggtttacg ccggaggaaa
     1141 tagcattgga cgccgccgcc atattggaag gggagcctgc ggcgccaccc gtgattctca
     1201 atgatcgcgc atatacgatt cgcgtccgct ttcccgaaca gagccgctct tcgctcgacc
     1261 gcatgagcaa tactctggtc accagcacca ccggccgcac ggccacgctc ggctcgttgg
     1321 ccacgatcat cgcggaacct gggcagacgg aaattcggcg cgaaaatctt cagcgtgttg
     1381 ttcaagtcac cgctcgattg gagggcacgg atcttggcag tggcatcgct gccgttcaga
     1441 aggctgtcaa cgatctgcat ttgcccgcgt ctatccgcgt cgagtacggt ggcttgtacc
     1501 aggagcagca gaaatcgttc cacgatctag tcatggtgtt gtttcttgcg ctcttgctgt
     1561 tgttcgtcgt gctcttgttc gaattccgca cgttctcggc gcccaccgcc attttagcct
     1621 cggcgttgct ctccactttt ggcgggttcc ttgcccttct cgtcacgcgc acgacattca
     1681 acgttgcctc cttcatgggt atgatcatgg tgatcggtat cgttgcaaaa aacggtattc
     1741 ttctgctcga tgccgaacac cacttccgcg atctgggtat atccgccgaa gatgccatga
     1801 ttcaggctgg gcgccggcgg cttcgaccca tcgccatgac cgcgctggcg accatcgcgg
     1861 gaatgttgcc attagccttc gccatcggcg ccggctcgca gatgctccaa ccgctggcca
     1921 tcacggtagt cggaggcatc ctgagctcca tggtgctctc tctcatcttc acacccgcga
     1981 tccattatta cctccagccg agaaagacga ccgaggcata aagcctgccg gcaaacactt
     2041 cggagctgaa tttctaagaa gcagtatggg gcgggaactg cccgaattga gactcgcaac
     2101 atggacatcg gcatcagcga taaagacctg ccgcatattt tcgagcggct ctactgcgcc
     2161 gatcaggctc gctcccgcga acccggcggc tttcgct
//
LOCUS       DASYVO010000207         4163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_19211, whole genome shotgun sequence.
ACCESSION   DASYVO010000207 DASYVO010000000
VERSION     DASYVO010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19211"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VGR03_06940"
     CDS             complement(<1..409)
                     /locus_tag="VGR03_06940"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994046.1"
                     /translation="MSVRLGEILVKESLITQDQLQKALEFQRANGGKLGSCLTKMGFI
                     TDDDITGVLSRQYGVPSINLKYYEIDPNVIKLIPQDTAARYQVIPLSRVGSVLTIAMT
                     DPTNVFAMDDIKFMTGFNVEPVVASESAIGDAIT"
     gene            730..2976
                     /locus_tag="VGR03_06945"
     CDS             730..2976
                     /locus_tag="VGR03_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1994047.1"
                     /translation="MSRLDNKQRWGLAIGGILLTLLLAAVFTFGSLNVPFEPKTWRTV
                     MTLYAVSSLITAAFLVFGLILARTILRLWVERSREQLGARFKTKMVVGAMAISLLPIV
                     FMFIVSYSLINRSLLLWFPKPLEIASDETQKLLNDLGRAQLPRLRGMALQARAETTLP
                     SADFLQHVFAKGADAVWILDRDGKAMRGGIVCDGQPEDRTGTICVQPDVPGKYLRSLP
                     SGVEVWAAGGRSYFAARVPASQAGQAEGFVVAASRTSPSVLDRLTAIQSQTREYYQEK
                     QDLRALKRQMLLILLLFTVLLLSAVMWVALFLAKQVTIPIQALAEGTREVSSGNFDYQ
                     VPEQAQDELGILVRSFNTMTMQLRDSRSQIDQFTRNLQQAVQELERRRQLMETVLENI
                     PTGVISLDAAGAILRVNTSVSKMFGVTSADPQSQSLEGLLGTDAARIVHYLMRRSLRM
                     GVVSREIETVAAGRVLHLAVTVSSLGPRRANTGYVLVLDDLTEVLRAQKSAAWQEVAR
                     RIAHEIKNPLTPIQLSAQRLLRFLERSDPSQSAATRHSELAKLVQECSRLIEREVSTL
                     AALVNEFSQFVRFPTAKLVPTNANTIVHQAVEVFSGRLDGITLKTALAENLPAVRADG
                     GLLRSVVVNLIDNAAEALENSAFREILVSTTAHSDAETVVICVSDTGTGISPEDKDKL
                     FLPHFSTKDRGTGLGLAIAARIVAEHGGSIHVEDNLPVGSRFLVELPAAELTPASLAD
                     HNGSDATR"
     gene            2973..>4163
                     /locus_tag="VGR03_06950"
     CDS             2973..>4163
                     /locus_tag="VGR03_06950"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV1994048.1"
                     /translation="MTPGPHLLVVDDEAGIRESLSSILEDEGYRVEAVGSAEEALERA
                     AAGDFEVVLLDVWLPGIDGLEALSRLQASPRPPAVIMISGHGTIETAVRATKLGAFDF
                     IEKPLSLEKIILLVRNAVQQRRLEEENQVLRSQLGYRYQVIGDSVPMKALRQQIAVTA
                     PTNGRVLIYGESGTGKELVARALHAASLRSKGPFVEVNCAAIPEELIESELFGHIKGS
                     FTGATEDKIGKFQKADGGTLFLDEIGDMSLRTQSKVLRVLEEQRFERVGSNETLSVDV
                     RVIAATNKHLEQEIARGAFRQDLFYRLNVIPFYVPALRDRKEDIPTLARYFLSEFSSA
                     YGKKTRELSGSALEVLLRYPWPGNVRELRNLVERLVIVCPQARIEPHHLPPELFRGAA
                     ESPHH"
BASE COUNT          809 a         1281 c         1212 g          861 t
ORIGIN      
        1 gcgtgatggc gtcgccgatg gccgactccg aggccaccac cggctcaacg ttgaacccgg
       61 tcatgaactt gatatcgtcc atcgcgaaga cgttggtggg atccgtcatg gcaatggtga
      121 gaacggaacc gacgcgcgac agcggaatga cctggtagcg cgctgccgtg tcttgcggaa
      181 taagcttgat gacgttggga tcgatttcgt agtacttcaa gttgatggaa ggaacgccgt
      241 actgacggga aagcacgccg gtgatgtcat catcggttat gaagcccatc ttcgtcaggc
      301 agcttcccag cttgcctcca ttggcgcgct gaaactccag cgctttttgg agctggtcct
      361 gcgtgatcag gctttcctta actaatattt cgcccagacg gacggacatc cgcagtactc
      421 ccgacgggtt tgcacccagc gctcacctta acaaatgcgg aagaggaagg tagccagaat
      481 ttcgcgcgcc gttctgaaat ggctaatacc tgcccacaag ggcaggtctc gcgaggagag
      541 gacaggtaga aatcttgact tgcctcggta cgctggctat cgtgccagag agatgtctgc
      601 acgcacaaca cggacacatt ctgctcatgc tctgacgccg ttgacgaaaa atgtccgcgg
      661 tgtgtacggc acatgcggct ttccattcca ggtgtacgtt tcactacgtg atttgatgat
      721 ggccgaatta tgagccgatt ggacaacaaa cagcgatggg gtctcgccat cggcggaata
      781 cttctgacgc tgctcctggc cgccgtcttc actttcggct cgctcaacgt accatttgag
      841 cccaagacct ggcgcaccgt gatgacactc tacgctgtct ccagtttgat caccgccgcc
      901 tttctcgtct ttggcctcat cctcgcgcgc accatcctgc ggctctgggt ggaacgcagc
      961 cgagaacaac tcggcgctcg ctttaagacc aagatggttg tcggcgcaat ggccatttcc
     1021 cttctgccga tcgtcttcat gttcatcgtg agctattcgt tgatcaatcg cagcctgttg
     1081 ctgtggttcc cgaaacccct ggagatcgcc tccgacgaga cgcagaaact cctgaacgat
     1141 ctggggcggg cgcagttgcc gcggcttcgg gggatggctt tgcaggcgcg cgcggagacc
     1201 actcttccca gcgcagattt cctgcaacat gtcttcgcga agggcgcgga tgccgtctgg
     1261 attctggatc gcgacggcaa agcgatgcga ggcggcatcg tatgtgacgg ccagccggaa
     1321 gatcgcaccg gcacgatttg cgtgcagccg gatgtcccag gaaagtattt gcgatcgttg
     1381 ccgagcggag tagaagtttg ggcggccgga ggaaggagct acttcgccgc tcgcgtcccg
     1441 gcttcgcagg caggccaggc tgaaggattt gtcgttgccg catctcgcac gagcccaagc
     1501 gttctcgatc ggctgaccgc cattcaatcc caaacgcgag agtattacca ggaaaagcag
     1561 gacctgcgcg cgctgaagcg gcagatgctt ttgattctcc ttcttttcac cgtgctgctg
     1621 ctgtccgcag tcatgtgggt ggcgttgttt ctggccaagc aagtcactat ccccattcaa
     1681 gcactcgcgg aagggacacg ggaggtttcc tccggcaact ttgattacca ggtcccggag
     1741 caagctcagg atgagttggg aatcctggtg cgctccttta acaccatgac catgcagttg
     1801 cgcgacagcc gctctcaaat cgatcagttc acccggaatc tccagcaagc ggtgcaggaa
     1861 ctcgagcgcc ggcgccagct catggagacc gtgcttgaaa acattcccac gggcgttatt
     1921 tcgctcgacg ccgcgggggc tattcttcgc gtgaatactt ccgtatcaaa gatgtttggc
     1981 gtgactagcg ctgatcctca atctcaatct ctcgaaggac tgctcggaac ggatgcggct
     2041 cgcatcgtgc actatctgat gcgcagatca ctgcgcatgg gggtggtctc ccgggagatc
     2101 gaaacggtcg ccgccggccg tgtgctgcac ctcgccgtca cggtgagttc cctgggaccg
     2161 cgccgcgcga acaccggcta cgtcctggtc ctcgacgatc tgacggaagt gcttcgcgcg
     2221 cagaaatcgg cggcttggca ggaagtcgcg cggcgaatcg ctcatgagat caagaatcca
     2281 ctgactccga ttcagctttc tgcccagcgc ctgctgcgct tcctcgagcg tagcgacccc
     2341 tcccaatcgg cggccacccg tcactcggaa ctggccaagc tggtccagga atgttcacgc
     2401 ctaatcgagc gggaagtctc cacgctcgcc gcgctcgtca atgagttctc gcaattcgtg
     2461 cgctttccta ctgccaagct tgtgccaacc aatgccaaca ccatcgtcca tcaggcggtg
     2521 gaagtattct ccggtcggct cgatggcatc acgctcaaga cggctcttgc cgagaatctg
     2581 ccggcggtac gcgccgacgg cgggctgctc cgcagtgtcg tcgtgaatct catcgataat
     2641 gccgcggaag cgctggagaa ctcggcgttc cgggagatcc tggtttccac cacggcccat
     2701 tcggatgcgg agaccgtggt gatctgcgtc tcggacaccg gcaccggtat ttcgccggaa
     2761 gacaaggaca agctcttcct gccgcatttc tccaccaagg accgcggaac gggactcggc
     2821 ctcgccatcg ctgcccgcat cgtggccgaa cacggcggca gcatccacgt cgaggacaat
     2881 cttccggtcg gatcgcgctt cctcgtggaa cttccggccg cggaattgac gcccgcctcc
     2941 cttgccgacc acaatggatc ggacgcgact cgatgactcc tggaccgcat ctcctcgtcg
     3001 tggacgatga ggccggcatt cgcgaatctc tttcctcgat tcttgaggac gagggctacc
     3061 gtgttgaagc ggtaggttcc gccgaggagg ccctggaacg cgccgccgcc ggcgatttcg
     3121 aggtcgttct cctggacgtc tggcttccgg ggatcgacgg cctcgaagcg ctcagccgtc
     3181 tgcaagcgtc tccgcgaccg cccgcggtca tcatgatttc cggtcacggc acaatcgaga
     3241 cagccgtgcg tgccaccaag ctcggcgcct tcgatttcat cgagaaaccg ctctcgcttg
     3301 agaagatcat ccttctggtt cgtaacgccg ttcagcagcg ccgccttgaa gaggaaaacc
     3361 aggtgctgcg aagccaactg ggttaccgct atcaggttat cggcgacagc gttcccatga
     3421 aggcgctgcg ccagcagatc gccgtgaccg cgccgaccaa cggccgcgtg cttatttacg
     3481 gcgagagcgg cactggcaag gagctggttg cccgcgcgtt gcatgctgcg agccttcgca
     3541 gcaagggccc tttcgtggaa gtcaattgcg cggcgattcc tgaggaactg atcgagtccg
     3601 agctcttcgg gcacatcaag ggcagcttca ccggcgcgac ggaagacaag atcggcaagt
     3661 ttcagaaggc cgacggcggg acgctcttcc tcgacgaaat cggcgacatg agcctccgca
     3721 cgcaatcgaa agtcttgcgc gttctcgagg aacagcgctt cgagcgcgtg ggctccaacg
     3781 aaaccctgag cgtcgatgtg cgcgtcattg ccgcaacgaa caaacatctc gaacaggaaa
     3841 tcgcgcgcgg cgcttttcgc caggatctct tctatcgcct gaacgtaatt cccttttacg
     3901 tgccggcctt gcgcgaccgc aaggaggata tccccacgct ggcaagatat ttcttgagcg
     3961 aattcagctc cgcctacggg aagaaaacgc gcgagctcag cggctccgcc ctcgaagttt
     4021 tgctccgtta tccctggcct ggaaatgtcc gggaactgcg aaacctcgtc gagcggctgg
     4081 tgatcgtgtg cccccaagcg cgtatcgagc cgcatcacct cccgcccgaa ctcttccgtg
     4141 gcgccgcgga gagtccccac cat
//
LOCUS       DASYVO010000208        12280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_90394, whole genome shotgun sequence.
ACCESSION   DASYVO010000208 DASYVO010000000
VERSION     DASYVO010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 12280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12280
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90394"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VGR03_06955"
     CDS             <1..1013
                     /locus_tag="VGR03_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1994049.1"
                     /translation="YLRVLPELNASVRQAVRECDAYILRMPGLLGRLALREINRLGRP
                     CALEVVGDPWDALGPGTWPSLLRPFFRRAAARQLRVACRHAMAVHYVTQQALQRRYPP
                     GEKAYAVGFSDALMDAAFASAEILEQRYGRIEQAARNAGDRGRRFRIGFIGSLARMYK
                     GPDLLLHAAADCRSRGLDFEMVMVGDGRHAQAMQALARRLGIADRTQFAGQLPYGQAV
                     FDFLDSIDLFVMPSRAEGLPRALLEAMARGCPCIGCEVGGIPELLAQDDLAPLGDAQA
                     LAGKILEVAGSPQRLKQMALRNLEKARQFSPEFLEEARRGFFRYVRDHSEARPANPRR
                     EA"
     gene            complement(1048..1779)
                     /locus_tag="VGR03_06960"
     CDS             complement(1048..1779)
                     /locus_tag="VGR03_06960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1994050.1"
                     /translation="MSLATADPIRPAQNLPRRAAAPQAPEDCYEDRLKQIIRPGDRIL
                     DAGCGTGKYFRASFARAAGCRLVGVDIQENLKSNSGIDFGVCADLQNLPFPDGSFEVV
                     NCRLAIEHVKTPEAALREFYRILKPAGRLAVFTSNLLHYYGVASSLTPHWFHVWFNSR
                     VRGFESADIFPTYYRANTQHRLQALFRKSGFGRAEVTLVEGAPSVLAFNSLLLSLGRA
                     YERLVNRHESLSRFRMNIIAVAYKL"
     gene            complement(1842..3407)
                     /locus_tag="VGR03_06965"
     CDS             complement(1842..3407)
                     /locus_tag="VGR03_06965"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1994051.1"
                     /translation="MNSNKMTRGWSLLRSEARVYPWKDTFLRMSLDFLLVNASFLAAV
                     ALWFLFYVVVLRVPDPQGMAERFKSFVVQYWLLWSSLALLVFQLTGMYPRTRGLRAAQ
                     KSVVVFRAVSLFIVLFIFADYFLYRGALAPRGVAALGWLLTLLAVGGSRLIKHGFLKS
                     YNVEHRTPPEKVKHVLVLGGAGYLGAVLVPQLLERGFRVRVLDSFLFGERSLDGVKLH
                     QHFELVRGDVRDIAAVVQAMRGCDAVVHLAAIVGDSACEENKQLAMEVNRAATRMLVD
                     VARGCGVRRFVFASSCSVYGASDLPVSEGSALAPLSLYAQTKVDSENILLAAKMDSLA
                     PTVLRLGTLFGLSGRMRFDLVVNLFVARAVSTGRITVLNGEQWRPFLHVRDAGHAIVE
                     CLEAAPRAVSGEIFNVGTPSLNLQIRGLGEAVARIIPSTTIDETENADRRNYRVSFEK
                     IQRTLGFQCQQSLEAGIKEMYGAIRSGVIGDLAPDRTNNQIAIRAVARASGGQLSPLG
                     QLAVLARVEGKRS"
     gene            complement(3419..4615)
                     /locus_tag="VGR03_06970"
     CDS             complement(3419..4615)
                     /locus_tag="VGR03_06970"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEV1994052.1"
                     /translation="MKPTAPDSRLSASNWPAFEKRLPIVSPEGLPGEEFLRDVRKILD
                     SRQLTNAAFVREFEDAAAAYLGVAHCVAVSSCTAGLVLTLRALDLRGEVILPSFTFHA
                     SAHSVVWNGLQPVFADCDPETFCLHPEAVRSRLSPDTAAILGVHLFGNPAFAAELEVF
                     ATGSGIHLIFDAAHAFGSRVNGRHVGTFGAAEVFSFSPTKLLVAGEGGLIATRDAALA
                     RRLRAARNYGDAGNYDPDLIGLNARMSEFHAALALRGLPGVDARIERRNQLRCQYQRR
                     LEAVPGLSFQPVRADSLSTWKDFSILVEEAAFGASRNWLWDALQKENIEARRYFWPPV
                     HRQKLYREIWDQRPLPVTDRISDCILNLPIYSSLTGEDVERVCGAILRASEFAKKHET
                     AKAQVA"
     gene            complement(4653..5327)
                     /locus_tag="VGR03_06975"
     CDS             complement(4653..5327)
                     /locus_tag="VGR03_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV1994053.1"
                     /translation="MMDVVILGAGGFARETRWVFLEDNEEKQKWNVLGFVDDKPEMQG
                     AVLCDLPVLGGFAWLERNAAKNFQVICGAGNPRTRKLFAERAASFGLSFCTLIHPSAR
                     MSRWVEVGAGTIITAGNILTTQIKLGTHTLVNLSCTIGHDTVIGAYCNINPGCQISGS
                     VKFGEGVDFGTGAVIIQGKSIGEWSMIGAGAVVTEDIPSHVTAVGVPCRVIKNHQPEA
                     SLTACV"
     gene            complement(5324..5971)
                     /locus_tag="VGR03_06980"
     CDS             complement(5324..5971)
                     /locus_tag="VGR03_06980"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1994054.1"
                     /translation="MSRSLQLGTKRVLDALVAAAGIVLLLPVFVLIAIAIRLGSAGQV
                     FFAQERTGLKGKRFRMLKFRTMVEDAQHTGSGLYVAKDDSRITRVGKTLRRFSLDELP
                     QLFHILVGRMSLVGPRPALPYHVEHYTARQARRLLMRPGLTGWSQVNGRNFLSWPERL
                     EKDAWYVDNFSLRLDARILIRTLAVLLSGEGLYGARDRFFFSGHDDIPAPSGRDS"
     gene            complement(5968..7383)
                     /locus_tag="VGR03_06985"
     CDS             complement(5968..7383)
                     /locus_tag="VGR03_06985"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV1994055.1"
                     /translation="MNGERTEANQQDWQVMVRIVEGILILGIAVAVLAFGGTEPVSFA
                     LVQVLFLGVAVLLLARPRGMTLAISARAFVVPAMLAGVVLLQLCPLPAGLLERLTGRD
                     YPLSGARLSSLSLDPFSTRTHFLILLTCLVAFYLAQIVGQDERRKKRLVVSLVALGTL
                     EAFYGLAQYLAGWQNIFVYVKRDDLEEATGTYINRNHYAGLLEMILPFSLALVFYEYG
                     ELRRNGAPPMSRARKMITGSTLQRLILWLGIAVVLFAAILFSRSRMGIVAASASQLAV
                     FGLAAAARFQEKTGLVLAASFILLSLGLAIWIGPGPILGRFESVGQEYTLGDESRLSI
                     WRDTGELIRQHPWLGTGLGTFPVAFTAVQTTFLGKFVNHAHDDFLELSSDLGIPAALA
                     LFVSILFVLARAVRSFLSAGRNFERVVALGCAGSLFAILLHSLADFNLYIPANALLFS
                     TVLGLTMTMQNGTSTEPGMPR"
     gene            complement(7380..9677)
                     /locus_tag="VGR03_06990"
     CDS             complement(7380..9677)
                     /locus_tag="VGR03_06990"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEV1994056.1"
                     /translation="MDQETNLRLYETSPHALEKRRAQRLSLHEAEEGQDVPDLLEYWR
                     VIHKRRATLLTILFVVFTVALFATLKEKAVYRARALIEIQKENADIPTLQELFQVENI
                     SDTYLETQYRILKSENLARRVISQLGLEHVAEFGPTVRDRAKIQQKQISAPAAGAVHG
                     ADPRNLDTDTEVLKKFQDRMSVEPLKRSRLVEVSFESNDPDLAAQVVNTLASTYMQQN
                     LEARWEASQRASEWLSQQLLEMKAKLEKSEDELQRYARENGLLFLETDKGTTENVVAQ
                     RLRELQEELTKVQAQRYAKESVYRLLQEGDFASLPGVFDNKLMQDLTEKLAELKRDRS
                     RLTSTFKTNYPRVQELQRQIDESEAELAAERERAAQLIRNDFRAAVEREQMLQTAFEE
                     QRKQVNDVAEKSVQYNILRREVDTNKQLYVGLLQKLKETGVSTTLNATNIRVVDPSYV
                     PKKPVRPRLLLNLSLALVLGLTLGIGAAFLQEHLDNTFKTAEDVERFLQLPALASVPA
                     VASSNGHGSVHGLYTRARLLAGDKERAIVVPQWNRIEGDGQNSALAEAFHGLRTSVLL
                     STPKHPPRTLLVTSAKVCEGKTTVAANLALSLAQLNEPVLLIDADLRRPSLYNFFGIT
                     QSSGLVDFLTGNPDWRSMVHHAAPTGLDVLFSGPVPPNPADLLSSEYMPTLIREASKD
                     YKFIVLDSPPLLNLADSRILATLVDGVILVIGGGITPRELVHRAYLSAVDAGSHVLGA
                     AINFAEPSASYYSYGYQRESDKSRG"
     gene            complement(9687..10952)
                     /locus_tag="VGR03_06995"
     CDS             complement(9687..10952)
                     /locus_tag="VGR03_06995"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEV1994057.1"
                     /translation="MFPQGSYCLLALLHQGFCAGSSGLLNRASVPKVFPRGASWSRLV
                     GTGRTLSALLLGVWFLFSPGVSRSQTAPGTIDRPGKDDQAETAEAYNRRLKKLRQSLA
                     ERTPTGQVAEYRIGAQDLLEINVFEAPELNRSLRVSAGGAISMPLLGEVQAAGLTARE
                     FEGVLQSRLSEFMNNPHVGVFVSAIESHPVSVAGAVKKPGVFQIREPMTLIEMISRAE
                     GLADDAGDTVLVMRSARFRPGADPGNERSEPSGAPSSSAGEVPQTRSVAPAADDSNEK
                     DTVRVNLKDLLESGDPRYDVAVHPGDVVKVARAGIVYVVGAVKKPGGFVIKSSEKMTV
                     LKAIALAEGLTGTSAKKRTQIIRTDASSGQRAEISIDLGKILAEKAPDPDLKPEDILF
                     VPNSPAKSVLYRGSEAALAIISGVIIFHR"
     gene            11508..>12280
                     /locus_tag="VGR03_07000"
     CDS             11508..>12280
                     /locus_tag="VGR03_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994058.1"
                     /translation="MICNARKGEWVKPLRLLTPGLALLAALAMVPRSAAQGRPYFVTY
                     DHHLEEPRSLEIALNPVIGSPAGGNGFLGSSMEFEYGVTGWWTSELYLDGQSTRREST
                     LFTGVRWENRFRPLLREHWINPVLYVEFENLNGADKALLEVVGFDGRANQAAPNSLAR
                     REKLHEIETKLILSSNFRGWNVSENFIAEKNLNGDPWEFGYAAAVSRPLALAASPRPC
                     RFCRENFYAGLEMYGGLGTAEKFTLSGTSHYLAPLLSWQ"
BASE COUNT         2512 a         3710 c         3711 g         2347 t
ORIGIN      
        1 aatatctgcg cgttcttccc gagctgaatg cttcggtgag gcaagctgtg cgcgaatgcg
       61 acgcttacat tctgcgcatg cccggccttc tgggccggct ggccttgcgt gagatcaacc
      121 ggctgggcag gccctgcgct ctggaagttg tgggtgaccc ctgggacgcg ctggggccgg
      181 gaacctggcc gagcctgctc cggccatttt ttcgtcgcgc ggccgcgcgc caactgagag
      241 tggcctgccg gcatgcgatg gcggtccact acgtcaccca acaggcgctt cagcggcgct
      301 atccccccgg ggaaaaagcc tacgcggtgg gattctccga tgcattgatg gatgctgctt
      361 tcgcttctgc ggagatcctt gagcagagat atgggcgcat cgagcaggcg gcgcggaacg
      421 caggagatcg cggcaggcga tttcgcatcg gctttatcgg cagcctggca cgaatgtaca
      481 agggtcccga ccttcttctg cacgccgccg cggattgccg ttcccgcgga ctcgacttcg
      541 aaatggtgat ggtgggcgac ggccgtcacg cccaggccat gcaggcgctg gcgaggcgcc
      601 tggggattgc ggatcgtacg caattcgccg gacagctccc ctacggccag gcggtctttg
      661 attttctgga ctcgattgat ctgttcgtga tgccgtcgcg cgcggaaggt ctgccacggg
      721 cattgctgga ggcgatggcc cgtggctgcc cctgcatcgg ctgcgaagtg ggaggtatcc
      781 ccgagctgct ggcccaggac gacctcgcgc ctctcggcga cgcgcaagcc ctggcgggca
      841 aaatcctgga ggtggccggc agcccgcagc gcctgaagca gatggcgctt cgcaatctgg
      901 aaaaagcgag gcagttcagc cccgaattcc tggaggaagc acggcggggt ttcttccgct
      961 atgttcgaga tcactcggaa gccaggccgg caaatccacg ccgcgaggcg tagagcctcg
     1021 cgcatcctcc tggtccggcg ggtgtgatca gagtttgtac gcgacggcaa tgatgttcat
     1081 gcggaatcgc gaaagagatt catgccggtt gaccagccgc tcataggcgc ggcctagact
     1141 gaggagaaga gaattgaacg cgagcacgct gggcgctccc tcgaccaggg ttacctcggc
     1201 ccgtccgaaa cccgacttgc ggaacagcgc ctgcaagcga tgctgcgtat tcgcccggta
     1261 gtaggtgggg aaaatatcgg cgctttcaaa tccgcgcacg cgcgaattga accacacgtg
     1321 gaaccaatgg ggagtcaagc tcgacgccac gccgtaataa tgcagcaggt tcgaagtaaa
     1381 gaccgcgagt cttcctgccg gcttcagtat gcggtaaaac tccctgagcg cggcttcggg
     1441 cgtcttcacg tgctcgatgg ccagccggca gttgacgact tcgaaggagc catccgggaa
     1501 gggtagattc tgcaaatccg cgcaaacccc aaaatcgatt cccgaattgc ttttcagatt
     1561 ttcctgaata tcaactccca ccaaccggca gccggctgcc cgggcaaatg aggcgcggaa
     1621 gtattttcct gtgccgcatc cggcgtcgag gatgcggtcc ccggggcgga taatctgctt
     1681 caggcgatct tcgtagcagt cttccggagc ctgcggcgcc gcagcgcggc gtgggagatt
     1741 ttgcgcgggg cggattggat cggcagttgc aaggctcatg atttatgcag gtcttcacag
     1801 ttcgagagaa tccgcgacga gggctggagg gttttgctcc ctcacgagcg ctttccctct
     1861 accctggcga gcacagccag ttgcccgagt ggagaaagtt gaccacccga agccctggca
     1921 acagcgcgga tggcaatctg attgttcgtc cgatccggtg caagatcgcc gatcactccc
     1981 gaacggatgg caccatacat ctccttgatt ccggcttcca aagactgctg gcattgaaat
     2041 cccagcgttc gctggatttt ttcaaacgag acgcggtagt tgcgccggtc ggcgttttcc
     2101 gtttcgtcga tggtggtgct ggggatgatg cgggcgacag cctctcccag tccgcggatc
     2161 tggagattca aggagggggt ccccacatta aagatttctc cggaaacggc ccggggagca
     2221 gcttcgagac actccacgat ggcgtgtccc gcatctcgca catgcaggaa aggcctccac
     2281 tgctcgccgt tcaacacggt gatccgtccg gtggagaccg cgcgcgcgac gaacaggttc
     2341 accaccagat cgaaacgcat gcggcccgac aacccgaaga gcgtgcccag acgcaacacc
     2401 gtgggagcaa gactatccat ctttgccgcc agcagaatgt tttccgaatc gacttttgtc
     2461 tgtgcgtaca gggagagcgg agccagagca gagccttccg agacggggag atccgaagcg
     2521 ccatacacac tgcaggagga tgcgaaaaca aagcggcgga cgccacaccc gcgagccacg
     2581 tccacgagca tgcgcgtggc ggcccggttt acttccatgg ccagttgttt gttttcctcg
     2641 caggcggaat cgcccacgat cgcggccaga tgaaccaccg cgtcgcatcc gcgcatggcc
     2701 tgcaccaccg cggcgatgtc gcgcacgtcc ccgcgaacca gttcaaagtg ttgatgcaac
     2761 ttcacgccgt caagcgatcg ttcgccgaag aggaaggagt ccagcacgcg caccctgaat
     2821 ccgcgctcga gcaactgagg caccaggacc gctcccaggt agccggctcc acccaggacg
     2881 agaacatgct tcactttttc cgggggagtc ctgtgctcca cattgtagga cttcagaaaa
     2941 ccatgtttga tcagacggct gccgcccacc gccagcaacg tcaacaacca accgagtgcg
     3001 gccacgccgc gcggcgcaag cgctccgcgg tagaggaagt agtcggcaaa gatgaataga
     3061 acgatgaaca ggctgacggc gcggaagacg acaacactct tctgcgccgc gcgcagaccg
     3121 cgggtgcgcg ggtacatccc agtgagctga aaaaccagga gcgccagcga tgaccagagc
     3181 agccaatact gcaccacgaa actcttgaac cgctcggcca tgccctgcgg atccggaacg
     3241 cgcaaaacga caacatagaa caggaaccag agagcgacgg cggccagaaa ggaagcgttg
     3301 accagaagaa aatcgagaga catgcggaga aaagtatctt tccaggggta gacgcgggcc
     3361 tcgctgcgaa gcaaggacca gccgcgcgtc atcttattgc tgttcatggg gctgttcttc
     3421 atgcgacctg cgccttcgcc gtttcgtgtt tcttcgcgaa ctcggaagcg cgaagaatgg
     3481 caccgcagac tctctcaacg tcttcgccgg tgagcgagga gtagatgggc aggttcagga
     3541 tgcaatcgga aatccgatcg gtgaccggca agggccgctg gtcccatatt tcgcggtaaa
     3601 gtttctgacg gtggaccggc ggccagaaat agcgccgcgc ttcgatgttt tctttctgca
     3661 gggcgtccca aagccaattg cgcgatgcgc cgaacgccgc ctcctccacg aggatggaaa
     3721 aatctttcca ggtgctgaga ctgtcagccc gcaccggctg aaagctgagc ccgggcaccg
     3781 cttccaggcg ccgctggtac tgacaacgaa gctgatttct gcgctcgatg cgcgcgtcaa
     3841 cgcccggcag gccgcgaagc gccagcgcgg cgtgaaactc actcatgcgc gcgttcaagc
     3901 cgatcagatc cggatcgtag ttgcccgcgt ctccgtagtt tcgcgcggcg cgcaaacgtc
     3961 tggccaaggc ggcgtcgcgg gtggcgatca agcccccttc gccggcgacc agcagcttgg
     4021 tgggcgagaa actgaaaacc tcggcggcgc cgaatgttcc cacatgccgt ccattgaccc
     4081 ggcttccaaa cgcatgggct gcgtcgaaga tcaaatggat tcccgagccg gtggcgaata
     4141 cttccagctc cgccgcgaac gcagggttcc cgaacagatg caccccaaga atggccgcgg
     4201 tgtccggcga cagccgggat cgcaccgcct ccggatggag acagaatgtt tccgggtcgc
     4261 aatccgcgaa caccggctgg aggccgttcc acacaacgct gtgagcgctg gcatgaaacg
     4321 taaaactggg aagaatcact tctccccgga gatccagtgc gcgcaacgtc agcaccaggc
     4381 ctgcggtgca actcgacacg gcgacgcagt gagccacgcc caggtaggcc gcggcggcat
     4441 cctcgaactc gcggacaaag gccgcgttgg tgagctggcg gctgtcaaga atcttgcgaa
     4501 cgtcccggag gaattcctct cccggcaggc cctcgggcga aacaatgggc agccgcttct
     4561 caaatgcggg ccagttgctt gcactcagtc gagagtctgg ggcagtgggt ttcatcgtct
     4621 ttccagaatt tcgcggctca ccgttttttc cgtcacacac aagccgtcag agaagcttcg
     4681 ggctgatggt tcttgatgac ccggcatggg acgccgacgg cggtcacgtg cgaggggatg
     4741 tcctcagtga ccacggcgcc cgcgccaatc atggaccact cgccgatgct cttgccctga
     4801 atgatcacag cgccggtgcc gaagtccact ccctcgccga atttcacgga acctgaaatt
     4861 tgacagcctg gattgatgtt gcaataggcg ccgatgacgg tgtcatgccc gatggtgcag
     4921 ctcaggttga ccagggtatg cgtgcccagc ttgatctgcg tggtcaggat attgcccgcg
     4981 gtgatgatcg tacccgctcc cacttcgacc cagcgcgaca tgcgcgccga cgggtgaatc
     5041 aaggtgcaga agctgagccc gaaggaggcg gcgcgttccg caaacagctt tcgcgtgcgg
     5101 gggtttcccg cgccgcaaat gacctggaag ttcttcgcag cgttccgttc cagccacgca
     5161 aagccgccca gcaccggcag gtcgcagagc acggcgccct gcatttcggg cttatcgtcc
     5221 acgaagccca gcacattcca tttttgtttc tcctcgttgt cttccaaaaa cacccagcgg
     5281 gtttcgcgtg cgaagccgcc ggcgccaagg atcacgacat ccatcatgaa tccctccctg
     5341 acggagccgg gatgtcgtcg tgccctgaaa aaaagaatcg atcgcgcgcc ccgtagagac
     5401 cttcacccga aagcaagacc gccagtgtgc gaatcaggat gcgcgcatcg agccggagag
     5461 aaaagttgtc cacgtaccaa gcatcctttt cgaggcgttc cggccaggaa agaaagttgc
     5521 ggccgttcac ctggctccag ccggtgaggc cgggccgcat caatagccgg cgcgcttgcc
     5581 gggccgtgta gtgctccacg tgatagggaa gagcggggcg agggccgacg aggctcatcc
     5641 ggccaaccag aatgtggaag agctgcggca gctcatcgag gctgaagcgg cgcagggttt
     5701 tcccaacgcg cgtaattcgt gagtcgtctt tggcgacata caggccggaa ccggtgtgct
     5761 gcgcgtcttc gaccatggtg cgaaatttca acattcgaaa ccgctttccc ttcaggccgg
     5821 tgcgctcctg cgcgaaaaag acctggcctg ctgagcccag cctgatcgca atggcgatca
     5881 aaacgaaaac gggcaagagc aggacaatgc ccgccgcggc cacaagggcg tcgagaactc
     5941 gcttggtgcc gagctggagg cttcggctca tcggggcatt cccggctccg tcgaggtccc
     6001 gttttgcatg gtcatggtca ggccgagaac cgtggagaaa aggagcgcgt tggcgggaat
     6061 gtacagattg aagtccgcca gactgtgcag cagaatggca aagaggcttc ccgcacatcc
     6121 gagcgcaacg actcgctcaa aatttcgccc tgcggaaagg aaactcctca ccgctcgcgc
     6181 gagaacgaag aggatggaaa cgaacaaagc gagcgccgcg ggaattccca ggtcgctcga
     6241 aagctccagg aaatcgtcat gggcgtgatt cacgaacttg cccaggaacg tggtctggac
     6301 tgcggtgaac gcaacgggaa aagttcccaa tcctgtcccc agccagggat gttgccgaat
     6361 cagttctccg gtgtcccgcc agatggatag ccggctctca tcgccgagcg tgtattcctg
     6421 tccgacgctt tcaaatctcc ccaaaatcgg ccccggcccg atccaaatgg ccaggccaag
     6481 actgaggagg atgaaagagg cggcgagaac aagccccgtt ttttcctgaa atcttgcggc
     6541 cgccgcgagg ccgaacacgg cgagctgaga cgcggaggcg gcaacgatgc ccattctcga
     6601 tcgtgagaaa agaatggcgg cgaaaaggac cacggcaatc cccagccaaa ggatcaggcg
     6661 ttgaagagtt gatccggtga tcattttcct cgcgcgagac atcggcggag ctccgtttcg
     6721 gcgcaactca ccgtactcgt agaaaacaag agccaggctg aacgggagga tcatctcgag
     6781 gagcccggcg tagtggttcc ggttgatgta cgtgccggtg gcttcttcga ggtcatccct
     6841 ctttacgtac acaaagatgt tctgccatcc ggcgaggtat tgagccagac cgtagaaagc
     6901 ttccagagtt ccgagagcaa ccagagaaac gaccagtcgc tttttgcgac gctcatcctg
     6961 accgacgatt tgagccagat aaaatgcgac caggcaggta agcagaatca aaaagtgcgt
     7021 gcgagtgctg aacggatcga gactcaaact gctcaggcgc gccccggaca acggatagtc
     7081 ccgccccgtg agccgctcca gcaacccggc gggaagcggg caaagctgaa gaagaacgac
     7141 accggccagc attgcgggca ccacgaaggc ccgggccgag atggccaggg tcatgcccct
     7201 aggcctagcg agcagcagaa ccgccacacc cagaaaaaga acctgcacga gcgcgaagga
     7261 aacaggttcg gtgccgccaa atgcgagaac ggccaccgcg atccccagaa tgagaattcc
     7321 ctcaacgatt cttaccatga cctgccaatc ctgctggttc gcttcggtcc tctcgccgtt
     7381 caaccgcgac tcttgtcact ctcccgctgg tatccatacg agtagtaact cgcgctgggt
     7441 tcggcgaaat tgatggcggc gccgagaacg tgggaacctg catccacggc agacagatag
     7501 gcgcggtgga cgagctcgcg cggagtgatt cccccgccga tcacaagaat gacgccatcc
     7561 accagcgtgg ccagaatgcg gctgtcggct aggttgagga gtggcggtga atccagcacg
     7621 ataaatttgt agtctttcga tgcctcgcgg atcagcgtgg gcatgtactc ggacgaaagc
     7681 aggtcggctg gattcggggg caccggcccg gaaaaaagca cgtccaaccc cgtcggcgcg
     7741 gcatggtgga ccatgcttcg ccaatcggga tttccggtca gaaagtcgac caagccggaa
     7801 gactgcgtga tgccgaagaa gttgtagaga ctcggccgcc gcaaatcggc gtcgatgagc
     7861 agcaccggtt cgttcaactg cgcgagagaa agagccaggt tcgcggccac cgtggtcttg
     7921 ccttcgcaca cctttgcact ggtcaccagc agagtcctgg gaggatgttt cggtgtggag
     7981 aggagcaccg aggttcgcaa tccgtgaaag gcctcggcca gggcggaatt ctgtccatcg
     8041 ccctcgatcc gattccactg gggcacgacg atggcgcgtt ccttgtcgcc cgccaaaagc
     8101 cgcgcgcggg tgtacagtcc atgcacgctg ccgtggccat tggaagaggc cacggcaggg
     8161 acggaagcca gcgcgggcaa ttgcaggaat cgctcgacgt cttcggcggt cttgaaggta
     8221 ttgtcgaggt gctcctgcag gaatgcggcg ccgatcccca gggtgagtcc gaggaccagc
     8281 gccagcgaca aattcagcag gaggcgaggg cggacgggtt tcttggggac gtaggacgga
     8341 tccaccactc tgatattcgt ggcgttcaaa gtggtggaga ctcccgtctc cttcaatttc
     8401 tgcagcagcc cgacgtacaa ctgcttattg gtatccacct cacgcctcaa gatgttgtat
     8461 tgcaccgatt tctcggccac gtcgttgacc tgcttgcgct gttcttcaaa tgccgtttgc
     8521 agcatctgct cgcgctccac ggcggcacgg aaatcattgc ggatgagctg ggcagcccgc
     8581 tcgcgctcgg cggccagctc cgcttcgctc tcatcgattt gtctttgcaa ttcctgcacg
     8641 cgggggtagt tggtcttgaa ggtcgaggtc agcctggagc ggtcgcgttt gagctccgcc
     8701 agtttttcgg tgaggtcctg catgagcttg ttgtcaaaga cgcccggcag ggaggcgaag
     8761 tcgccctcct ggagcaggcg atacaccgat tccttggcgt atctttgggc ctgtactttg
     8821 gtcaattcct cctgcaactc ccgaagtctc tgggcaacga cgttctcggt ggtccccttg
     8881 tccgtttcca gaaacagcag cccgttttca cgggcgtatc gctgcagctc gtcttccgac
     8941 ttttccaact tcgctttcat ttccagcaac tgctgcgaga gccactccga agctctctga
     9001 gaagcctccc agcgagcttc caggttctgc tgcatgtagg tggaggccag ggtgttcacc
     9061 acctgtgcgg ccagatccgg atcgttggac tcgaaactga cctcgaccag acggctgcgc
     9121 ttcaatggtt ccacgctcat gcgatcttga aacttcttca atacctcggt gtcggtgtca
     9181 aggttccgag gatccgcgcc atgaacggcg ccggccgccg gcgcggagat ttgtttctgc
     9241 tgaatcttgg cccgatcccg cacggtgggg ccaaactcgg cgacgtgctc gagcccaagc
     9301 tgggaaatta cgcgccgcgc gagattttcg cttttcaaga tccggtactg tgtctccagg
     9361 taggtgtcgg agatgttctc gacttgaaaa agctcctgca gcgtcggaat gtcggcgttt
     9421 tctttctgaa tctcgatcag cgcgcgggcc ctgtacacgg ccttctcttt gagcgtggcg
     9481 aaaagcgcga ccgtaaaaac gacgaataga atggtcagca gggtggcgcg gcgcttgtgg
     9541 atgacccgcc agtactcgag gaggtccggc acatcctggc cctcctccgc ttcatgcaag
     9601 gacagacgct gcgcgcggcg cttttccagg gcgtggggcg acgtctcgta caaccgcagg
     9661 ttggtttcct ggtccatttt cttttcttat ctgtggaaaa tgatgacgcc gctgatgatg
     9721 gcgagagcgg cttcacttcc ccggtacaga acggatttcg ccgggctgtt cgggacaaag
     9781 agaatgtcct caggtttcaa gtccggatcg ggcgcctttt ccgccagtat ttttcccaaa
     9841 tcgatggaga tttcggcccg ctgcccgctg cttgcatcgg tacgaatgat ttgcgtccgt
     9901 ttcttggccg acgttcctgt caagccttcg gcaagcgcga tggccttcag aaccgtcatt
     9961 ttctcgctgc tcttgattac gaatccgccg ggcttcttca ccgcgcctac gacgtagacg
    10021 attcccgccc gggctacctt gacgacgtcg ccgggatgga ccgcgacgtc atatcgaggg
    10081 tcccccgact ccagcaggtc cttcaggttt actcggacgg tgtctttctc attcgagtca
    10141 tccgccgcgg gggcgacgga gcgagtttgc ggcacctcgc cagcggagga ggacggagcg
    10201 ccgctcggtt cgctgcgctc attccccgga tccgcgccgg gacggaatct tgcgctccgc
    10261 ataaccagca cggtgtcccc ggcatcatcg gcaaggcctt ccgcccgaga aatcatctcg
    10321 atcaaagtca tgggttcccg gatctggaac accccgggtt tcttcaccgc ccccgcaacc
    10381 gataccggat ggctctcgat cgcgctgacg aacacaccaa cgtgaggatt gttcatgaac
    10441 tcactcagcc tggattgcag gacaccttcg aactctcgcg ccgtcagccc ggcggcctga
    10501 acctcgccca acagcggcat cgagatcgcg cctcccgccg atacccggag cgagcgattc
    10561 aattccggcg cttcgaagac attgatttcg aggagatcct gcgccccgat tcgatactct
    10621 gcgacttgac cggtaggtgt tcgttccgcc agcgactgtc gcagtttttt gagacgccgg
    10681 ttgtaagcct cggccgtctc cgcttgatca tcctttcccg gtcggtcgat ggttcctggg
    10741 gctgtctgcg agcgtgaaac tcccggcgaa aacagaaacc agacaccgag cagaagggcg
    10801 gagagggttc gtccagttcc gacaagcctg ctccatgaag cgcctcgggg gaagacctta
    10861 ggaacactag ctctgttgag gagccccgag gaaccggcgc agaaaccctg gtgaaggaga
    10921 gcgagaagac aatacgaccc ctgcggaaac atcgtggccc ccaagagtgt cccaagcccg
    10981 gcgagttctg ccgcgtcaac ttccgcggca agcagctctc agcactgcgc gcctgaacgc
    11041 aggttccccg cttcgcccct tgccaggaca gccgtgactc cgtcaagagt cagattcaga
    11101 ttccgcggct cgctgggcga cacccatacg cacatgaccc agcagattcg gcaggagtgt
    11161 aagcccagac gtacccagaa tgtcaagcgc cgcggatgtt cccaacgacc aactcttgat
    11221 gtggctggcg gtgatggcgc gttttcctcg ccgcagcgcc ggccaagccg tgccgtgagg
    11281 agatccggct caaaatcatt ctcctgaatt cttcacggca gggaagtact ttcagcattc
    11341 tgagagtatt ctccttcgcg aaattctccg cccggaggct ctccggcgcc attgaaccgg
    11401 tcgtctgctc cattccctga aagcagatat gtccaaggga agtcatgggt gtattgtagt
    11461 tgcaaatgat ttgcaatacg gaaatgaagc aaccatttta gatgcaaatg atttgcaatg
    11521 ctagaaaagg tgaatgggta aagccactgc gtctcctgac tccgggactt gcgcttctcg
    11581 ccgctctggc gatggtgccc cggagcgcgg cgcaggggcg cccttatttc gtgacctacg
    11641 accaccatct cgaggaaccg cgcagcctgg agatcgcgct caaccccgtg atcggatcgc
    11701 cggccggggg gaacggcttt cttggctcct cgatggagtt cgaatacgga gtcacgggct
    11761 ggtggacatc ggagctttac ctcgacgggc aatccactcg ccgcgaaagc accctgttca
    11821 cgggagtgcg ctgggagaat cgtttccggc cgctgctcag ggaacattgg atcaatcccg
    11881 tgctctacgt cgagttcgaa aacctcaacg gggccgacaa ggcgcttctc gaggtcgtcg
    11941 ggtttgacgg ccgggcgaac caagccgcac ccaattcgct ggcgcgccgc gaaaagctgc
    12001 atgagatcga gaccaagctg attctctcca gcaattttcg tggttggaac gtctccgaga
    12061 acttcatcgc tgagaaaaac ctgaatggag atccctggga attcggatac gctgccgcgg
    12121 tcagccgccc gctggcgctc gcggcttcgc cgcgaccctg ccgcttctgc cgggagaatt
    12181 tttacgccgg tctggagatg tacggcggac tgggaacggc ggagaaattc acgctctccg
    12241 ggacgtcgca ctatcttgcg ccgcttctct cctggcagat
//
LOCUS       DASYVO010000209         3234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_106135, whole genome shotgun sequence.
ACCESSION   DASYVO010000209 DASYVO010000000
VERSION     DASYVO010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106135"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..517)
                     /locus_tag="VGR03_07005"
     CDS             complement(170..517)
                     /locus_tag="VGR03_07005"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEV1994059.1"
                     /translation="MKVSERRQLARLNLRTPLRFRTLGVAADKTEHFTEALNVSRGGF
                     FFASSAPLQVGMPIEATLRMPAEVTGNTSQEMRCTARVVHVRNEPYGDGRIGFGAEIE
                     KFQTIPTQDRWVV"
     gene            801..1811
                     /locus_tag="VGR03_07010"
     CDS             801..1811
                     /locus_tag="VGR03_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEV1994060.1"
                     /translation="MNTAPSTESAGYQPPSWDSILAAHARIAPRIHRTPILTSASLNA
                     MTGAQLFFKCENLQKTGSFKIRGASNAILSLTEKEAAQGIVTQSSGNHGAAVACAAAW
                     RGVPAWIVMPKNAPAVKAKAIEGYGGRIVFCEPTVTARKASCDRVQAETRAHLVHPYD
                     DDRIIAGQATAAKELLEEVGDLDAVFAPVSGGGLLSGTCLGAKGIRSDVRVFGCEPER
                     ADDAYRSLTTGTLQSQDSSDTIADGLRASLAPRTFAILQRHVDRILLVTEEEIIASAR
                     LVWERMKIIIEPSSAVAIAPLLRRGVVASLNLPKRADGGAPKLGVIFSGGNVDLSSLP
                     WK"
     gene            complement(1848..2666)
                     /locus_tag="VGR03_07015"
     CDS             complement(1848..2666)
                     /locus_tag="VGR03_07015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1994061.1"
                     /translation="MTRYNDKQKVRQFYDRVSPYSQNLWGMHLHHGYWISGEETKEKA
                     QIQLVEHLADLIGLGPGSKILDVGCGYGGSSIHLAKNYDADATGITISPIQVEMARKA
                     AALDGVNAKFILMDAEKMEFDESFDVVWSVESISHYPHKEKFFASAAQCLKSGGTLAI
                     IDWFKKEQLTQREYKKFIQPIEKGMLVELDAMEDYAVLLRTNGFHVVRDEVLSKNCSK
                     TWDLCLDMLKNKALWRLAAENGSEFVDFLLAFRAMRAGFASGNFVYGLVIAKRI"
BASE COUNT          690 a          959 c          911 g          674 t
ORIGIN      
        1 gtgtcatcct gagcgaagcg aaggatctca actgacacgc ctcctcgcaa tcaagtcggg
       61 gctggtctgc acacccttaa ttacaggacg ctcctcgccg gccactctag gaaccatttc
      121 tatctgtcat tgaactgatt actgaaaact gaggactgac gactactctt cagacgaccc
      181 agcggtcttg ggtggggatg gtttggaatt tctcaatttc ggcgccgaag ccgatgcgac
      241 cgtctccgta aggctcgttg cgcacatgaa cgacgcgcgc ggtgcagcgc atctcctggg
      301 aggtatttcc ggtcacttcc gcgggcatgc gcagggtcgc ttcgataggc attcccactt
      361 gcaggggagc cgatgaagcg aagaagaagc cgccacgcga gacgttcaga gcttcggtaa
      421 aatgctcggt cttgtccgcg gctaccccga gtgtgcggaa gcgcaggggc gttctcaggt
      481 tgaggcgtgc caattggcgg cgttcagata ctttcatttt ttcctcccca gcgcgaggaa
      541 acttctggcg cgggtactcg cggatttagt ggaggggact tccagcgtaa cagagccagc
      601 ggagcggtca agtaccaaac ctggcatggc caagtgatac cagtacccgc atgtaatgcg
      661 tgcgcgagtg gaacatccgc gcggacgcag ccggttccag catgccataa tgccccttta
      721 acagcggcga acaacgcatt ataagtgggc gcgataaacg tgaagaagtg gcaagcgttt
      781 cctggcccag attgagcctt atgaacacag ctccatccac cgaatctgcc ggctaccagc
      841 cgcccagttg ggactccatc cttgcggcgc acgcgcgcat cgcccccagg atccaccgca
      901 cgccgatcct gacctccgca tccctgaacg cgatgaccgg ggcacagctc ttcttcaaat
      961 gcgaaaacct gcaaaagacg ggctcgttca aaattcgcgg ggcctcgaat gcgatcctct
     1021 ccctgacgga gaaagaggcg gcgcaaggca tcgtcacgca atcgagcggc aatcatggcg
     1081 ctgccgtggc ttgcgcggct gcgtggcggg gagttccggc ctggatcgtt atgccgaaaa
     1141 atgcgcccgc cgtgaaagcc aaggccattg agggctatgg cggaagaatt gttttctgcg
     1201 agcccacggt gaccgcgcgg aaggcaagtt gcgaccgcgt ccaggcagaa acacgcgcgc
     1261 atctggtaca tccctacgac gacgaccgca tcatcgccgg acaggccacc gctgcgaagg
     1321 agctactgga agaagtcggc gacctggacg ccgtgttcgc gcccgtcagc ggtggcggat
     1381 tgctcagcgg gacgtgcctc ggggccaagg gtattcgctc ggacgtgcgc gtgtttggtt
     1441 gcgagccgga gcgcgccgat gacgcctacc gctccctgac caccggtacg ctgcaatcgc
     1501 aagactccag cgacacgatc gccgacggct tgcgcgcctc gctggccccg cggactttcg
     1561 cgatcctgca gcgccatgtg gatcgcatcc tcctggtcac cgaggaggag atcatcgcat
     1621 cggcgcggct ggtctgggag cgcatgaaaa tcatcattga gccatcgagc gccgtggcca
     1681 ttgccccgct cctgaggcga ggagtggtgg catcgttgaa tctgccgaaa cgcgccgacg
     1741 ggggcgcccc gaagctcggc gtaatcttct ccggcgggaa cgtggacctc tcttcccttc
     1801 cctggaagtg acggctgtcg accaagagcg gtttattgag aacctgctta gattctcttg
     1861 gcgatgacga gtccgtagac gaaatttccg gaggcgaagc cagctcgcat ggcgcggaag
     1921 gccagcagaa aatcgacaaa ctccgacccg ttttccgctg ccagccgcca gagcgcttta
     1981 ttcttgagca tgtccaggca cagatcccaa gttttggagc aattcttgct caaaacctca
     2041 tcgcgcacga catgaaagcc gttcgtcctg agcaataccg cgtaatcttc catcgcgtcc
     2101 agttcgacga gcataccctt ttcaattggc tggatgaatt tcttgtactc gcgttgtgtg
     2161 agctgctcct ttttgaacca atcgataatg gccagggtcc caccagattt cagacactgc
     2221 gcggctgaag caaaaaactt ttccttgtgc ggatagtggg agatggattc gacggaccaa
     2281 accacatcga aggattcgtc aaattccatc ttctcagcgt ccattaagat aaactttgcg
     2341 ttcacgccgt cgagggccgc tgctttccgg gccatttcca cttgaatggg agagatcgta
     2401 atccctgtcg catcggcgtc gtagtttttg gccagatgga tactgctgcc cccataacca
     2461 cagccaacat cgaggatctt acttccaggc ccgagaccta ttaaatccgc gagatgttca
     2521 acaagctgaa tctgcgcctt ttcttttgtt tcttccccgc taatccaata gccatgatgc
     2581 agatgcatgc cccaaagatt ttggctatag gggctcaccc ggtcgtagaa ttgcctgacc
     2641 ttttgtttgt cgttatagcg cgtcatggat tcgcagtgcc tcgccccaac agatcagttc
     2701 gctagtcgcc actgccgacc gcatatatta tcccacgcaa agcgtgagag ctaggcgctg
     2761 caaattggtt gcgcccgcgc accgtccaaa ttgctgcaca atttgtgcag gtaataaggc
     2821 acattatacc agggccccat ccggcgcagg ctttcatatt gaaatcacag gacttaaatc
     2881 atggagagtt tggacgaccg catgctccca tgtacggcgg aaggaatggg tttaagaatg
     2941 agcatgaata tccaagaacg agtcggggag caccgcgcag cggtactact gggactcccg
     3001 atgacagaaa tccggcgctt ttcgcgtata tccggcctgg gacacctgga agcaggtgac
     3061 cgaggcgagc aagtggtttt cacgtacgag gaactgcgga gactctgcct gctcgccgca
     3121 cagtcttcga agtagttgcc acgctctcgc aagagtttgc ccagactcct cctcactccc
     3181 cccggcctcg ggtgcctccc cacaggcact cggggccttc ctccgaccgg attc
//
LOCUS       DASYVO010000210         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_74699, whole genome shotgun sequence.
ACCESSION   DASYVO010000210 DASYVO010000000
VERSION     DASYVO010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74699"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1086
                     /locus_tag="VGR03_07020"
     CDS             58..1086
                     /locus_tag="VGR03_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEV1994062.1"
                     /translation="MPEPRFLVVRLGSLGDIVHTFPAVAGLRESFPEAEIVWLTHPRW
                     KALVESSELATDIWETETRSYRSLCEIIGRIRKEHFTTAIDYQGLWKSAALPFFGGVS
                     RRIGFSSQTIREFGVPILFTDRVRCTQTHIADQNGELSRRAGARNSVAPVALAVPSIQ
                     EVFVLQLLRGFAIERYVVLSPAGGWRSKCWPPDRYGALCQKIRGTLALRCVLNYGPGE
                     EDLVSVVKAASGEADPIAYNGSLGQLMALLRNAVCVVGGDTGPLHLAVAIGTPAVAIF
                     GPTDPARNGPYRMGDGSGNASPIDIVLRSPQAVTTYKRGDQAAPSILEIDVDTVFDAV
                     RRQVEVRR"
     gene            1083..1640
                     /locus_tag="VGR03_07025"
     CDS             1083..1640
                     /locus_tag="VGR03_07025"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEV1994063.1"
                     /translation="MSAAGNFFVRWRVRLGYPLAVAVLYFAWPSPRSILLGALVGVIG
                     LCVRAYAAGYLHKQEVLTVTGPYAYTRNPLYLGSAVLALGAGIAARSWISASILIVYF
                     TVFYSMVMRREAEELHLRHGASFEEYARAVPLFIPRLSAAKLPGDSAGSFSFAQYKKN
                     HEWQAAVGFLFLLFVLLVIWRFRPH"
     gene            complement(1590..>2256)
                     /locus_tag="VGR03_07030"
     CDS             complement(1590..>2256)
                     /locus_tag="VGR03_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994064.1"
                     /translation="HLGGLPAILDNFRVAKLWIGREVSSPALVRLEELAGERNIPIEH
                     ELRGKSFRWDGVDGDFLWPVIAPEEVAPSAKNNDSLVLRLRYGSQTFLLPGDAEKQVE
                     REIISENSAEAMHSDVLKIGHHGSKNSTMPEFLAAVQPRFGIISAGEDNPYGHPSPEL
                     LERLENAGVRVLRTDRDGAVHVLTDGARLEITCFVACPVLANAAASVRPEAPNHEQDK
                     EKK"
BASE COUNT          406 a          712 c          624 g          514 t
ORIGIN      
        1 gtgatcccaa ggatcactcc acgcgagagt tgctagactc catccgcaag gcgccgcatg
       61 cctgagccgc gcttcctggt cgtgcgcctc ggttctctcg gagatatcgt ccacaccttt
      121 cccgctgtcg ccggcctgcg cgagtcgttt cccgaagctg aaatcgtttg gctgacgcat
      181 ccccgctgga aagccttggt cgagagcagc gaactggcaa cggatatttg ggagacggaa
      241 actcgctcct accgctcctt gtgcgaaatc atcggacgaa tccgcaaaga acacttcacg
      301 accgcgatcg attatcaagg gctgtggaag tctgcggcgc tgccgttctt cggcggcgtc
      361 tcgcgcagga tcgggttttc ctcgcaaacc attcgcgagt tcggcgtgcc gatcctcttc
      421 accgatcgcg ttcgttgcac acagacccac atcgccgatc agaatggaga actttcacgg
      481 cgcgccggcg ctcgcaacag cgtggcgccc gtggctctcg cggtgccgtc gattcaggaa
      541 gtgttcgtgt tgcagcttct gcgaggtttc gcgatcgaac ggtacgtcgt actaagtccc
      601 gcgggcggct ggcgctcgaa atgctggccg ccggatcgtt acggcgctct ctgccagaaa
      661 atccgtggca ctctcgcact gcgttgcgtt ctgaattatg gccccggtga agaggatctg
      721 gtctccgtgg tcaaagcggc cagcggggaa gctgacccca tcgcttacaa tggttcactc
      781 ggccaactca tggctcttct gcgcaatgcg gtctgcgtcg tcggtggtga tacgggaccg
      841 ctgcatctgg ccgtcgctat tggaacgcct gctgtcgcca tctttggccc gaccgatccg
      901 gcgcgaaacg gtccctatcg catgggagac ggctcgggca acgcttcgcc catcgacatc
      961 gttctgcgca gtccgcaggc agtgaccacc tacaaacgag gcgaccaagc cgctccttcc
     1021 attctcgaaa ttgacgtcga caccgttttc gacgccgttc gccggcaagt tgaggttcgc
     1081 cgatgagcgc ggccggcaat tttttcgttc gctggcgtgt acgcctcggc tatccgcttg
     1141 cggtagcggt tctttatttt gcttggccct cacctcgcag tatcttgctt ggcgcactgg
     1201 tgggagtcat tggtctgtgc gtgcgcgcgt atgcggccgg ctatcttcac aaacaagaag
     1261 tcctcaccgt caccggtccc tacgcttaca ctcgaaatcc gctctatctc ggcagcgccg
     1321 tcctggccct aggggccgga attgcagcgc gctcctggat ctctgcctca atcctgattg
     1381 tttacttcac cgtgttttat tccatggtga tgcggcggga agcggaagag ctccacctgc
     1441 gccacggggc atctttcgaa gagtacgcgc gcgccgtccc gctctttatt ccgcgcctga
     1501 gcgctgcgaa actacctgga gactcggccg gttccttctc tttcgcccag tacaagaaga
     1561 atcatgaatg gcaggccgcc gttggctttt tatttcttct ctttgtcttg ctcgtgattt
     1621 ggcgcttccg gccgcactga cgctgccgca tttgccaaaa ctggacacgc aacgaagcaa
     1681 gtgatctcca gccgcgcgcc gtccgtcaac acatgcacgg cgccgtctcg atccgtcctc
     1741 aagacgcgca cgcccgcatt ctccaatcgt tcgaggagct ccgggctggg atggccgtaa
     1801 gggttgtcct cacccgcgga tatgattccg aaacgcggct gtacggcagc aaggaattcc
     1861 ggcatcgtcg aattcttgct tccgtgatgg ccgatcttca gcacatcgga atgcatcgct
     1921 tccgcgctat tttccgaaat gatttcgcgt tccacctgtt tttccgcatc gcccggcagc
     1981 aggaaagttt ggcttccgta gcgcaaccgc agtaccagag aatcgttgtt ctttgctgat
     2041 ggtgcgacct cttccggagc aatcaccggc cacaaaaagt cgccatcgac gccatcccag
     2101 cgaaacgact tgccgcgaag ctcatgctct atagggatat ttcgctcgcc cgccagttct
     2161 tccagtctca cgagggccgg ggaagatact tcacgcccga tccagagttt tgcaacgcga
     2221 aaattgtcca aaatggcagg aagccccccc agatga
//
LOCUS       DASYVO010000211         4866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_49432, whole genome shotgun sequence.
ACCESSION   DASYVO010000211 DASYVO010000000
VERSION     DASYVO010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49432"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1916
                     /locus_tag="VGR03_07035"
     CDS             <1..1916
                     /locus_tag="VGR03_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994065.1"
                     /translation="MSIKQRDPQAFRQLIGYDIPTSQIIKYGTAALREASGGKGPFAG
                     IPPGGTYLEGMLFKELGSGDAGENNRIGKALYGLYANNQTNPETGKPATLDEVKRAAF
                     NQQHAADVPNGDKLYPKGTLWDAATQASAIKRSAPEFLTWLGARGITLVDKIDQEVAQ
                     ARKEWAALPWGGGDSGVLKADLNAFAAKYPWYSALTLSNKDSQDRNALFAGDVLSRIP
                     PGSRTAAYDTAGVKELVNGFYAAKGPLAKSDYLNNLPADQQQKFMQFITATDKQYASP
                     TQAQQASFAAGTKFWDSYNQMDPLARQMLRALPEAQKLNLDSLLNKDSRASYPVSSFD
                     QLAQLAADFSARLTSNKSYQAYQQEFSQHGFSDKTLALESGYFAAGAQGSAERRQYLT
                     ANPDLKSYFAWRANFMAQHPDLALASQNKGGLPATTAVASGGATTKGAPTAGLSQASA
                     AAYKDFYAARDARYPNVDIKVLSEQYAALSPGATRTQFLNANPQLKDYFQWHTDYLNK
                     TPAFAAASAEAKHLYQPAATSDPSVIRAAAALAPMARELGSYFSLRAGGGGGSYGGGG
                     RGGGASLSRRGTALLNTVARTYTPQLNTTQLLSALEPFYKLENKGAGGTGGGGGAPFL
                     RNVRYIKPYSRGR"
     gene            1932..2132
                     /locus_tag="VGR03_07040"
     CDS             1932..2132
                     /locus_tag="VGR03_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994066.1"
                     /translation="MSNSIDPEGVQLLSVELMLTPSGYDSPRKVYLPKQLSLRQLADY
                     RRDFSPEDFERIKQACGNPRAA"
     gene            2212..2949
                     /locus_tag="VGR03_07045"
     CDS             2212..2949
                     /locus_tag="VGR03_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994067.1"
                     /translation="MSNEIAAQAGVDAENPNGVASAATNQAQAGQLPEDETAKKVRLL
                     EEQFNTLRSVKDRQVSDAERRARDAEGALAAQQSETVQREQAAYDAWAANAEPEDKLK
                     AELERANRQWALHATALNRERMNSEKWRAAAQYGLDPAKVEAAQSPTDLNNLIASKQA
                     EAAKTEMRDSIRKEVEAEYAKRTGQPAAQQPAKQAGKIVEMRGQDGGVSSLTKFQQAT
                     QDFEDKKISKVEFAKIRADFQRNMPSE"
     gene            complement(3322..3864)
                     /locus_tag="VGR03_07050"
     CDS             complement(3322..3864)
                     /locus_tag="VGR03_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV1994068.1"
                     /translation="MTKPSRYNKFHYKLGKKCPDCGKPIMDKATRCWDCTWIDRRLHP
                     KKRVWRCTYCGIQITADHKYCKKCSAQHRLERAGKQPKVSKDRGYKNLFKPGHPNAHA
                     NGALQEHRFIVSEAIGRPLKPREVVDHKDKNKRNNGPANLLLFATTRDHQVYEYIRDF
                     GIPVPIWDGANPTVVKERDS"
     gene            3934..>4866
                     /locus_tag="VGR03_07055"
     CDS             3934..>4866
                     /locus_tag="VGR03_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994069.1"
                     /translation="MNGEKGNVVQFSTFEQTDVATATLSETADIPPVPLKDSAVVITL
                     AEYGNALVSTKFLKATSFNDIEKAATANMAVNRARSLDLVCRAQIIAGINVFRPNGRV
                     TRATLQNPADIISFAFLTQATSRAKTLNMPPTDMGDFVAPINPILLADLVNISQWQNV
                     SAYQQDGQEIYEGEVGKLVGVRFVQTYTGKVYLSGGTIRQAATTTTASINAGDTACPI
                     TSGTGLNNGDFVTIGTLESSVAEQVLITAGGLTTTLTIQGVGNSQSNFGFRYAHASGV
                     AVTKADQVAALPLMGAQSVAKAYSDLTGPRGQVVL"
BASE COUNT         1090 a         1392 c         1507 g          877 t
ORIGIN      
        1 ccatgtcgat caagcagcgc gacccacagg cgttccgcca gcttatcggc tacgatatcc
       61 cgaccagcca gattatcaag tatggcacgg cggcgctgcg agaggcatca ggcggcaaag
      121 gcccgttcgc gggcatcccg cccggcggga cctatctcga agggatgctg ttcaaggagc
      181 tgggttcggg cgacgcgggc gagaataatc gcatcggcaa ggcgttgtat gggctatacg
      241 cgaataacca gactaacccg gaaacaggga agcccgccac tctggacgaa gtgaagcgcg
      301 cggccttcaa ccagcaacac gcggcggacg ttccgaacgg ggacaagctc tatcccaagg
      361 ggacgctctg ggatgcggcg acgcaggcaa gcgccatcaa gcgttcggcg ccggagttcc
      421 tgacctggtt gggtgcgcgc gggattacgc tggtagacaa gatcgaccag gaggttgcac
      481 aggcgcgcaa agagtgggcg gcgctgccgt ggggcggtgg cgattcggga gtgctcaagg
      541 cggacctgaa cgccttcgcg gcgaagtacc cgtggtactc cgcgctaacc ctttcgaata
      601 aggacagcca ggaccgcaat gcgctctttg cgggcgacgt gctttcccgc atcccgccgg
      661 gctcgcggac ggcggcttac gatacggccg gcgtgaagga actcgtcaac ggtttctatg
      721 ccgccaaggg cccgctcgcc aagtcggact atctcaacaa cctgcccgcc gaccagcagc
      781 agaagttcat gcagtttatt acggccactg acaaacagta tgcgagcccg acacaggcgc
      841 agcaggcttc gttcgcggcc ggtacgaagt tctgggacag ctacaaccag atggacccgc
      901 tggcgcgcca gatgctgcgc gccctacccg aggcgcagaa gctcaatctt gattcactct
      961 tgaacaagga ctcgcgcgcg agttatcccg tctccagctt cgaccagctc gcgcagctcg
     1021 ccgcggactt tagcgcgcgg cttacgagca ataaatctta ccaggcatat cagcaggaat
     1081 tttctcagca cgggtttagc gacaagacgc ttgcgctgga gagcggttac ttcgcggcag
     1141 gggctcaggg cagcgccgag cgtcgtcagt atctcacggc gaacccggac ttgaaatcgt
     1201 actttgcctg gcgagcaaac ttcatggcgc agcatcctga tctggcactt gcgagccaga
     1261 acaagggggg gctgccggcc acgacggcgg tcgcgagcgg aggcgcgacc acgaaagggg
     1321 cgccgaccgc cggtctctcg caggcctcgg cggcggctta caaggatttc tatgcggcgc
     1381 gtgatgcgcg ctacccgaac gtggacatca aggtgctgtc agagcagtac gccgcgctgt
     1441 cgccgggggc gacgcggacg cagttcctga acgccaaccc gcagctcaag gattacttcc
     1501 agtggcatac cgactacctg aacaagaccc cggcttttgc ggccgcaagc gcggaagcga
     1561 agcatctcta tcagccggcg gcgacttccg acccgagcgt gattcgcgcc gcggctgcgc
     1621 tcgcgcctat ggcaagagag ctgggttcct acttctccct gcgtgcgggg ggaggcggtg
     1681 gtagttacgg aggcggaggg cgtggtggtg gggcttccct ctcgcgccga ggcaccgcgc
     1741 tgctgaacac cgtggcgcgc acgtatacgc cgcaactgaa cacgactcaa ttgttgtcgg
     1801 cgctggaacc gttctacaag ttggagaata agggagcggg cggcacgggc ggcggcggcg
     1861 gagcgccgtt cctaagaaac gtgcgctaca tcaagccgta ttcgcgaggc cggtaagagg
     1921 acaggcaaat aatgtccaat agcatcgacc cagagggcgt acagcttttg agcgtggagt
     1981 taatgctgac cccatcaggc tacgacagcc cccgcaaggt gtacctgcct aagcaattgt
     2041 cgcttcgcca actggcagac taccggcggg attttagccc cgaagatttt gaacgcatca
     2101 agcaggcgtg cggcaacccc cgcgccgctt gacaagtggg cagctaagta gtaaagtttt
     2161 tctaagttag ccggttagga ccggcacagc aaagcaaaat acaggagagg catgtccaac
     2221 gagattgccg cacaagctgg agttgacgcc gaaaatccca acggggttgc atcggcggcg
     2281 acgaaccaag cgcaggccgg ccagttgcca gaggacgaga cggcgaagaa ggtacgcctg
     2341 cttgaagagc agttcaacac gctgcgaagc gtgaaggacc gtcaagtgag cgatgccgag
     2401 cgccgggcca gagacgcgga gggggcactc gccgcacaac agtccgagac ggtacagcgg
     2461 gagcaggccg cgtatgatgc gtgggcggca aacgcagaac ccgaggacaa gctgaaagcg
     2521 gaattggagc gtgcgaaccg ccagtgggcg ctgcacgcga ccgcactcaa cagggaacgg
     2581 atgaattcgg aaaagtggcg ggcggcagca cagtacggac tggaccctgc aaaggtcgag
     2641 gccgcgcaga gccccaccga tctgaataac ctgattgcgt cgaagcaggc cgaagcggct
     2701 aaaacggaaa tgcgtgacag tatccgcaaa gaggtcgagg ccgaatacgc aaagcgcaca
     2761 gggcagccgg cggcacagca gccggcgaag caggccggca aaattgttga gatgcgcgga
     2821 caggatggag gtgtttcgtc cctgaccaaa ttccagcagg cgacccagga tttcgaggat
     2881 aagaagatct ccaaggtcga gtttgcaaaa atacgcgcag actttcagcg gaacatgccc
     2941 agcgagtaat gctacttcct gactctggac tctccctttg agaccgtcaa accagtcaaa
     3001 aaggagagca tccaatggca ggtgttgtca caactaccgc tctgttaacg aacagcgtaa
     3061 aggcgctgta cgattcagag ttccagatgg ctgcaaccgg tgaagaggtc cacgatggac
     3121 tgaccttaaa tgtgagggtc cggcagtagg aatactgctg aaaaattcgc gtgaagtcgg
     3181 aagaagcctg agatggtaat tccgagagaa ggtttcaata ccctcgtaga gaatatggac
     3241 gcgacaccca gaccgggtga agatttattc cgatctgcaa ctaaatgaag ttgcagaggc
     3301 cggcagaaat gaccggcccg atcaagagtc tcgctcttta acaacagtag gatttgcacc
     3361 gtcccagatg ggaaccggga taccaaagtc gcggatatat tcgtagacct ggtgatcgcg
     3421 ggtagtggca aagagtagca ggtttgcagg gccgttgttc cgcttgttct tgtctttgtg
     3481 atccacaacc tctcgcggtt tgagcgggcg gccaatggct tcggagacaa tgaagcgatg
     3541 ctcctgcaag gctccattcg catgggcgtt tggatggccg ggcttgaata ggtttttgta
     3601 gccacggtcc ttgcttacct ttggttgctt gccagccctt tccagacggt gttgagccga
     3661 acacttcttg caatacttgt ggtcagcggt aatttgaatg ccacagtatg tacagcgcca
     3721 cacacgtttt ttgggatgca ggcgacgatc tatccaagtg caatcccaac agcgcgtggc
     3781 cttgtccatg ataggtttcc cgcagtcggg gcacttcttg ccgagcttgt agtggaactt
     3841 attgtagcga gatggtttcg tcatgcgggg atcctactgt atgttaacca gttagtcaat
     3901 tcttgattcg taacattagt caccaaaaag gttttgaacg gagagaaagg caacgtcgtt
     3961 cagttcagca cgtttgaaca gacggacgtg gcgactgcaa ctctctccga gaccgcggac
     4021 attcccccgg ttcccctcaa ggattccgca gtcgttatca cgctggccga gtacggcaac
     4081 gcgctagtca gcaccaagtt cctgaaggcc acatccttca acgacattga gaaggccgcg
     4141 accgcaaaca tggcggtcaa ccgcgcacgc tcgctggacc tggtgtgccg cgcgcagatc
     4201 atcgccggca tcaacgtctt tcgtcccaac gggcgcgtga cacgcgccac gctgcaaaat
     4261 cccgccgaca ttatttcctt tgcgtttctg acgcaggcga cttcccgcgc caagacgctg
     4321 aacatgccgc caacggacat gggcgatttc gtggcgccca tcaatcctat cctgcttgcc
     4381 gatctggtga atatctccca gtggcagaac gtctccgcgt accagcagga cggtcaggaa
     4441 atctacgaag gcgaagtggg caagctggta ggcgtgcgct tcgtccagac gtacaccggc
     4501 aaggtgtact tgtcgggcgg cacgatccgg caggccgcga ccaccacgac cgcttcgatc
     4561 aacgccggcg ataccgcgtg cccgatcacg tccggcaccg gtttgaacaa cggcgacttc
     4621 gtgacgatcg gcacgctgga atccagcgtg gccgagcagg tcctgattac ggccggcggt
     4681 ctgaccacga ccctgactat tcagggtgtg ggcaactcgc agagcaactt cggtttccgc
     4741 tatgcccatg cctccggcgt ggccgtgacg aaagccgatc aggttgcggc gctcccgctg
     4801 atgggcgcgc agtcggttgc gaaagcctac agcgatctga ccggaccccg cggccaggtg
     4861 gtgttg
//
LOCUS       DASYVO010000212         1830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_118059, whole genome shotgun sequence.
ACCESSION   DASYVO010000212 DASYVO010000000
VERSION     DASYVO010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118059"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VGR03_07060"
     CDS             <1..1100
                     /locus_tag="VGR03_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994070.1"
                     /translation="ETVYLDQVRLLAIDHPAAYEVFPNERFASTPPFPEFRVIASRDA
                     HPPAGAWDGRGNDVLPLVAKRDRKYVTSFEEMPFSGFAKLHWLELDLGDWDAKKPLRL
                     IIDGYTDYFTATSMYAADQAGIKVIPPYVEALDAQGKWVRIVEDMGFPAGLERTMVAD
                     LTGKLPPGTRRVRIVNNLKIYWDAIGIDQTPDTQDVRVAQVPLAKAALDFVGYPRETR
                     LQPASDTVYSYSHRSLTGPYAHAAGNYTRYGDVYDLLRSSDDRFVVFGSGEGLKLDFD
                     PRKLPALPAGWVRDYFFYADGFEKDLDFYAAHAFTVEPLPHHSLLPYPYPEGKDYPTD
                     AEHLGYQLEYNTRTRSDRMPSSLRYHYSSPR"
     gene            complement(1111..>1830)
                     /locus_tag="VGR03_07065"
     CDS             complement(1111..>1830)
                     /locus_tag="VGR03_07065"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1994071.1"
                     /translation="QPRLVHGSFLQDWLLFPTDWNWRLDPKLGGELRAVSDIGTHWLD
                     LMMWITGRKVMEVCADLATVVPVRQRPRGRVETFQKASNIEHDDVPITTDDYASVLLH
                     FEDDLRGVMTVSQVSAGRKARLWFEIDGSKGSLAWNSESPNTMWIGRRDRASEVLPKD
                     PALLSPAARGFAAYPGGHAEGYPDTFVQLFRDFYGYLDAGDMNALRPFPTFAIGNHEL
                     ILCEAIAKSAQNLAWVPVLWP"
BASE COUNT          375 a          527 c          538 g          390 t
ORIGIN      
        1 aagaaacggt ttatctggat caagtgcggc tgctggccat cgatcatccg gcggcttacg
       61 aagtttttcc gaatgagcgc ttcgccagca cgccgccttt cccggagttc cgcgtcatcg
      121 cgagccgcga tgcacatcca ccggcgggcg cgtgggacgg ccgcggaaat gatgtgctgc
      181 cgctcgtcgc aaaacgcgat cgcaagtacg tcacgagttt cgaagagatg cctttctcgg
      241 gattcgctaa gttgcactgg ctcgaacttg atctcggtga ttgggacgcg aagaaaccct
      301 tgcgtttaat catcgacgga tacaccgatt acttcacagc gacgtcgatg tacgcggcag
      361 atcaggctgg aatcaaagtc attccgccgt atgtcgaagc cctcgatgcc cagggcaagt
      421 gggtgcgcat tgtggaggac atgggctttc ccgcggggct tgagcgcacg atggttgcgg
      481 atttgactgg gaaactacct cctggtacgc gccgcgttcg aatcgtcaat aacctcaaaa
      541 tttattggga cgcgatcggc attgaccaga cgcctgatac ccaggacgtg cgcgtggcgc
      601 aagtgccgct cgcgaaggcc gcgctcgatt tcgtcggata tccgcgcgag acgcggctcc
      661 agcccgcgag cgacacggtt tattcctact cacatcgcag cctgaccgga ccgtacgccc
      721 atgcagcggg gaattacacg cgctatggag atgtttacga tttgttgcgt tcttccgatg
      781 accgcttcgt tgtttttggt tcgggcgagg gactgaagct tgattttgat ccgcgcaaac
      841 ttcccgcgtt gcccgcaggc tgggtccgcg actatttctt ttatgccgat ggcttcgaga
      901 aggatctgga tttttatgcg gctcacgcgt tcacggtgga accgctgccg caccattcgc
      961 tgctgcctta cccctatccg gagggcaaag attatcctac agatgcggag catcttggat
     1021 accagcttga atacaacacg cggactcgat cggatcgcat gccgtcgagc ctgcggtatc
     1081 actattcttc gccgcgctaa tgctagacaa ctaaggccac aataccggga cccaggccag
     1141 attctgagcg ctctttgcga tggcctcgca aagaatcaac tcgtggttgc cgattgcaaa
     1201 tgtcgggaat ggccgaaggg cgttcatgtc tcccgcatca aggtatccgt aaaaatcgcg
     1261 gaagagttgc acgaaggtgt cgggataccc ctcggcgtga ccaccgggat aggccgcgaa
     1321 acctcgcgcg gccggagata gcaaggcagg atccttgggg agcacttcgc tggccctgtc
     1381 gcggcgtcca atccacatcg tatttggaga ttcagaattc caggcgagtg agcccttgct
     1441 gccgtcgatt tcaaaccaga gcctggcctt gcgcccggcg ctgacctgcg agacggtcat
     1501 cacaccgcgc agatcgtctt cgaagtgcaa gaggaccgag gcatagtcat ccgtggtgat
     1561 cggaacatcg tcgtgttcga tgttcgacgc cttctgaaat gtctcaacgc ggccacgggg
     1621 ccgctgcctg acggggacga cggtggccag atccgcgcat acttccatta ctttgcggcc
     1681 ggtaatccac atcatcaggt cgagccagtg cgtgccgatg tcggaaacag cgcggagctc
     1741 cccgccaagc ttcggatcga gccgccagtt ccaatccgtc gggaagagca gccaatcctg
     1801 gagaaaactt ccgtggacaa ggcgcggttg
//
LOCUS       DASYVO010000213         2517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_118065, whole genome shotgun sequence.
ACCESSION   DASYVO010000213 DASYVO010000000
VERSION     DASYVO010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118065"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VGR03_07070"
     CDS             <1..715
                     /locus_tag="VGR03_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV1994072.1"
                     /translation="QNAIVRIGRAFGLQPHRVENFKFSKDPQFVEKVRDIVDLYLNPP
                     DRAIVLCVDEKSQVQALNRTEPILPLAPGIPARQSHDYERHGVTSLFAAMDVASGVTI
                     SNCYRRHRHQEFLRFLNEIEDNLPARLDVHLVMDNYGTHKVAKVRSWLARHPRYHVHF
                     TPTSGSWLNLVERLFAEVTERCVRRGSHTAVRVLEKAMLDYLDQRNRNPKPFVWTADA
                     DLILRKVERLCKRISRSGH"
     gene            complement(898..2208)
                     /locus_tag="VGR03_07075"
     CDS             complement(898..2208)
                     /locus_tag="VGR03_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045934309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEV1994073.1"
                     /translation="MGKSYRPYYPDEDLLLPPSLRDWLPEKHLAYFVSDVVDHLDLSA
                     MDAVYGNEKRGQPPYDPQMMTKVLVYGYCVGVFSSRRIERRLVEDIAFRVLAAGNQPN
                     FRTISDFRKIHLKTLEGLFEQVLKIALEAGAMKVGRVAVDGTKVKANASKHKAMSYDR
                     IKEKEKDLKAEVKQLLEQAEAADAEEDAAYGKDQRGDELPAELERRETRIKKIREAKR
                     ALEARAREKAAAEGADPKGAKPKEKDQYNFTDPESRIMKGADGFVQAYNAQAAVEPDL
                     QLIVGQTVTPAANDKEQLLPMVEVMEQQSGQRPEEILADSGYCSEKNLEALDSEENSE
                     RRIDGYVATERQKHGEYKEPCPRGPLPKGATRVDRMRRKLKTKAGKAVYAARKAIVEP
                     VFGQIKQARGFRQFLLRGIDKVRGEWSLVCLTHNILKIYRLCYE"
BASE COUNT          472 a          764 c          674 g          607 t
ORIGIN      
        1 gcagaacgcc atcgtacgga tcgggcgtgc tttcggattg cagccgcatc gtgtggaaaa
       61 cttcaagttt tccaaagacc cccaatttgt ggagaaggtg cgggacatcg tggacttgta
      121 cctgaatccg ccggatcgcg ccatcgtatt gtgtgtggat gaaaagagcc aagtgcaggc
      181 gctgaatcgg accgaaccga ttctgccgct ggccccgggc atccccgcgc ggcagtcgca
      241 tgattacgag cggcatggag tcacgtctct gtttgcggct atggatgtag catccggcgt
      301 gaccatcagc aattgctacc gccggcaccg gcatcaggag ttcttgcgat ttctcaatga
      361 gatcgaggac aatcttcccg ccaggctgga cgtgcatctg gtgatggaca actacggcac
      421 acacaaggtc gccaaggttc gaagttggtt ggcgcgacac ccgcgctatc acgttcactt
      481 cacgccgacc agcggaagct ggctcaacct ggtagagcga ctcttcgcag aggtgaccga
      541 gcgctgtgtg cgtcgaggca gtcacactgc cgttcgagtg ctggaaaagg ccatgctcga
      601 ttatttggac cagcgcaatc ggaatcccaa gcccttcgta tggaccgccg atgcggattt
      661 gattcttaga aaggtcgagc gactttgtaa acgcatttct cgctcaggac actaggagtc
      721 cgtccgagaa acagttttcg tatttcgacg agttagttcg ggaggattca aaataggatg
      781 ctgtcggagt gcccctacga ttgaaaagcc gaatcagtgg ttcatgtgcg aaaaacacga
      841 tcagattgac ccggcgagga tgactcttcg tggttttgcg gcgcggcttt gccggtttta
      901 ctcatagcag agccggtaga tcttcagaat gttgtgcgtg aggcacacga gtgaccactc
      961 cccccgcacc ttgtcgatac cacgcaatag aaactgccgg aatccgcgcg cctgcttgat
     1021 ctggccgaac accggttcca caatcgcctt gcgggccgcg tacaccgctt tgcccgcctt
     1081 ggttttcagc ttgcgtcgca tgcgatcaac ccgcgtggct cccttcggca gcggaccgcg
     1141 cgggcagggt tccttgtact cgccatgctt ctgccgttcc gtggcgacgt atccatcgat
     1201 gcggcgttcc gagttctcct ctgaatcaag tgcctccagg tttttctccg agcaataacc
     1261 gctgtcggcg agaatctctt ccggcctctg cccgctctgc tgttccatga cttccaccat
     1321 cggcagcaac tgctctttgt cgttggccgc cggcgtcacc gtctgaccca cgatcaactg
     1381 caagtccggc tcgaccgccg cctgcgcgtt ataggcttgc acaaagccat ccgcgccttt
     1441 catgatgcgc gattccggat cggtgaagtt gtattgatcc ttctctttcg gtttggctcc
     1501 tttcggatcg gcaccctccg ccgccgcctt ctcgcgcgcc ctcgcttcca gcgcgcgttt
     1561 ggcttcccgg atcttcttta ttctggtctc gcgccgctcc agttccgccg gcagttcgtc
     1621 gccccgctgg tctttgccgt aggccgcgtc ttcttccgca tcggcggctt cggcctgttc
     1681 cagtaactgc tttacttcgg ctttgagatc tttctccttc tctttaatcc ggtcatagct
     1741 catggctttg tgcttgctgg cgttggcttt gactttcgtc ccgtccaccg cgacacggcc
     1801 caccttcatg gccccagctt ccagggcgat cttcaaaacc tgttcgaaca gaccttccaa
     1861 cgtcttcagg tgaatcttgc ggaaatcgga gatcgtgcgg aagttcggtt gattgcccgc
     1921 cgccaggacc cggaaggcta tgtcttccac cagccggcgt tcgatccggc gtgaactgaa
     1981 cacacccacg cagtagccgt ataccagcac cttggtcatc atctgcggat cgtagggcgg
     2041 ctggccgcgc ttctcgttcc catacaccgc gtccatcgcc gacagatcca ggtgatccac
     2101 tacgtcgctg acgaaatacg ccagatgttt ctccggcagc caatcacgca ggctcggcgg
     2161 cagcagcaga tcttcatccg ggtagtacgg acggtagctt ttgcccatag aacattatgg
     2221 gatcatcctg tttcagagcg ccaacaaaaa ctctgtcgtg acatcgattt ctcggacggg
     2281 ctcctagtcg cgtacaggag tctagcaagc tccaggtagt cgtcggcatc cagggacccg
     2341 cctttgatct tctcgcgcaa ttgcctctct tcgaataatg cgttcatatt tcgcatttga
     2401 atagagattg cggctcaggc gccgatatct tcattccaaa gcgccggatt ggacttgatg
     2461 aattcggaca tcagccggat gcagtcgtcg tcctgaacga cttccaccag aattccg
//
LOCUS       DASYVO010000214         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_118069, whole genome shotgun sequence.
ACCESSION   DASYVO010000214 DASYVO010000000
VERSION     DASYVO010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118069"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VGR03_07080"
     CDS             <1..465
                     /locus_tag="VGR03_07080"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1994074.1"
                     /translation="CYPITTSRFIFGEEPVRVAGLIERDPDFKTDRLASAILDFPSGQ
                     AVFTCSTQLVSYQRMQFLGTKGRIEMEIPFNAPPDRATRIFIDDGRDVFGGGIRTETI
                     PVCDQYTVQGDAFSRAIREGSEVPVPLEDAIANMAVIEAVFRAGESGRWENP"
     gene            603..1169
                     /locus_tag="VGR03_07085"
     CDS             603..1169
                     /locus_tag="VGR03_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1994075.1"
                     /translation="MAIRTVLQLGDPGLREVANRVQDPAAPEIRSLVEDLADTLAYWR
                     KTTGYGRAIAAPQIGAKVRVIFLQIPGEEPWPLVNPVISERSKEKIVVWDACLSFLSI
                     FMQVERHREITVRYQVLSGEQREIHAGVERNLSELLQHEIDHLDGILAVDRITDMHTM
                     CTREEFEKRYRAGSPYAVEAAGKETPNS"
     gene            1221..1712
                     /locus_tag="VGR03_07090"
     CDS             1221..1712
                     /locus_tag="VGR03_07090"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV1994076.1"
                     /translation="MSGVFYTTFESPVGLLLLAGDSNALRRVSFENSKRSVPPQPDWK
                     QNRAAFAGVIRQLHAYFRGELKEFDLPLAMEGTEFQLRVWNALRAIPYGETISYAQLA
                     QRIDNPKAVRAVGLANGSNPIPIIVPCHRVIGSDGSLTGFGGGLSTKKKLLALESKQL
                     SLL"
     gene            complement(1815..>2582)
                     /locus_tag="VGR03_07095"
     CDS             complement(1815..>2582)
                     /locus_tag="VGR03_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV1994077.1"
                     /translation="GALAGLEYIAIFSAETHNPARNLPRAIALTALPIAFLYIFGTSG
                     ILAFVSPNSADMVAPIPQALSLGLQSLRFASVLAPLSIGFLLINYLSTFSLNFAGNTR
                     LPMVAGWDHLLPEWFTRLHPRYRTPVNSTLFLGAFAAVASLGALIGVGEQEAFELLQI
                     LAFTFYGIAYLAMFAIPLLARKETGLRPQLWLRLAAFSGFLVTLLFVFLSAFPIIPVA
                     DQTVYTLKTVSVILGANALGLLLYRVGSRREIRSAGE"
BASE COUNT          624 a          631 c          820 g          507 t
ORIGIN      
        1 tgctacccga ttacgacgtc gcgctttatt tttggagagg agccggtgcg cgtggcggga
       61 ctgatcgaaa gagatccgga ttttaagacg gaccgattgg cgtcggcgat tctggacttt
      121 ccgtcggggc aagcggtgtt cacgtgcagc acgcagttgg tgtcctacca gcggatgcaa
      181 ttcctgggga ctaaggggcg gatcgagatg gagataccgt ttaacgcgcc gccggatcgt
      241 gcgacgagaa ttttcataga tgatgggcgc gatgtatttg gcgggggaat tcggacggag
      301 acgattccgg tttgcgatca gtatacggtg cagggggatg cgttttcgcg ggcgattcgg
      361 gagggaagcg aggttccggt gccgctcgag gatgcgattg cgaacatggc agtgattgag
      421 gcggtttttc gggcggggga atcggggaga tgggagaacc cgtagaacat ttcaacacag
      481 agagcatagg gaatacagag tagaaagatg aaaggaagaa gcaaagacag gcgaagagtt
      541 taacgcagag acgccccgac ccggtcgggg tagacgccgg atgcagagag agaaagacgg
      601 aaatggctat acggactgta ttgcaattgg gtgatccggg attgcgcgaa gtggcgaacc
      661 gcgtacagga tccggcggca ccggagattc ggtcgctggt ggaggacctg gcggatacgc
      721 tggcgtattg gcgaaagacg acgggatatg ggcgcgcgat tgcggcgccg cagattggcg
      781 cgaaggtgcg agttattttt ctacagatac cgggcgagga gccttggccg ctcgtaaatc
      841 cggtgatctc cgagcggagc aaagaaaaaa tagtggtgtg ggatgcgtgc ttgagtttct
      901 tgtcgatctt catgcaggtg gaacggcacc gagaaatcac cgtgcggtat caggttctaa
      961 gcggtgagca gcgcgaaatt cacgcgggcg tggaacgaaa cctctcggaa ctgctgcagc
     1021 acgagatcga tcatttggat gggattttgg cggtggatcg cattacggac atgcacacga
     1081 tgtgcacgcg cgaagagttc gagaagagat atcgcgcggg gagtccgtac gccgtggaag
     1141 ccgctggcaa agaaactccg aattcataga agtaggatcg cgagacgcac gattgtcggg
     1201 ccttgcaagg aaactagagg gtgagtggcg tgttttacac aacgttcgaa agtcccgttg
     1261 ggctgttgct tcttgcgggc gattcgaatg cgctgcgccg ggtgagtttc gagaacagca
     1321 agcgttccgt gcctccacag ccggactgga agcagaatcg ggccgcattt gccggtgtca
     1381 tccgccaatt gcatgcgtac ttccgcggcg agttgaagga attcgatctg ccgctggcga
     1441 tggaaggaac ggaatttcaa cttcgcgtgt ggaatgcgct gcgtgcgatt ccctacgggg
     1501 aaacgatttc ctacgcgcaa cttgcccagc gcatcgacaa tcccaaagcc gtgcgcgctg
     1561 tgggattggc gaacgggagc aatcctattc ccatcatcgt gccatgtcat cgcgtcatcg
     1621 gcagtgatgg cagcctcacg ggtttcggcg gcggtctctc aaccaagaag aagttactcg
     1681 ctctcgaaag caagcagtta agtttgctgt aatcaattct tgccaatcag aaaaacggtg
     1741 gcctgccacc aacaggccgc tgtcttttta ccgagacaga tgccgctcta ggataatgat
     1801 gtcactcgcc gccgctattc gcccgccgaa cgaatttctc tgcgcgaacc cacacgataa
     1861 agcagcaatc ccagcgcgtt cgctccgaga atgacggaaa ccgttttcaa cgtatacacc
     1921 gtttgatcgg ccaccgggat gattgggaac gccgagagaa aaacaaatag tagcgtaaca
     1981 agaaatccgg aaaaggccgc gagtcgcagc cataactgtg gccgcaatcc ggtttctttt
     2041 ctcgccagaa gcgggatcgc aaacatcgcc agataggcga tgccataaaa tgtgaacgcc
     2101 aaaatctgca gcaattcgaa cgcttcctgc tccccgacac cgatcaatgc acccaggctc
     2161 gccacggcgg caaatgctcc gagaaacaag gtcgaattca ccggtgtgcg gtatcgcgga
     2221 tgcaatctgg taaaccattc tggcagaaga tgatcccatc ccgcgaccat cggaagcctg
     2281 gtattcccgg caaaattcag gctgaaggta gacaggtagt tgatcagcag gaacccaatc
     2341 gagagaggtg cgagcaccga ggcgaaccgc aaactctgaa gtcccaggct gagcgcttgc
     2401 ggtatcggtg caaccatgtc cgccgagttc ggagagacga aagcgagaat cccgcttgtt
     2461 ccaaagatat acaagaaggc gatgggcaga gccgtgagcg cgatcgcgcg cggcaggttt
     2521 cgcgcggggt tgtgggtttc cgcggaaaaa attgcaatgt actcgagtcc ggcaagcgca
     2581 cc
//
LOCUS       DASYVO010000215         3082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_74751, whole genome shotgun sequence.
ACCESSION   DASYVO010000215 DASYVO010000000
VERSION     DASYVO010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74751"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            476..961
                     /locus_tag="VGR03_07100"
     CDS             476..961
                     /locus_tag="VGR03_07100"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEV1994078.1"
                     /translation="MCKHQKGFSLIELLIVVAIILIIAAIAIPNLLRSRMSANEASAV
                     GSLRTINTAEIAYSTTYPDLGFAGLTALGGVAPCTVATPASACLIDDVLSSAAAVKSG
                     YGFTVTNLTGAPVVVTYTATALPVAVGQSGQRAFCSDQSGVVRYNDTGTACDATADPA
                     L"
     gene            complement(1120..2133)
                     /locus_tag="VGR03_07105"
     CDS             complement(1120..2133)
                     /locus_tag="VGR03_07105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994079.1"
                     /translation="MLTRLSLLLVSMMLVTSGLLRDAKPAPPAGGLLVVANQKEHTLL
                     VVNPDNRRELAKIAVGVNGHEVIVSKDGRLAYVPIYGNSGVGRPGTDGSTIDIVDLQE
                     HKLAATIDMGKPLRPHRAEFGPDGLLYVTAELANAVDVIDPSTNKVLAEIPTGEPQSH
                     MLVLSRDGHRAYTANVSAGSVSVLDLAKRNLITTIPVAKSVQRISISPDGRRIFTQDQ
                     DAPRIAVIDTATNRITNWIALPDVAYASAPTPDGRWLLAVSLAANRLHVVDLQTLKVV
                     RSLEVPEKSSEILIRPGGDIAYVSGTGAGKIAVLDLHSWKMQQTIDLTPGVDGLAWVP
                     ASQ"
     gene            complement(2205..2618)
                     /locus_tag="VGR03_07110"
     CDS             complement(2205..2618)
                     /locus_tag="VGR03_07110"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV1994080.1"
                     /translation="MTVDWLREVCLSFPGAAEQIQWGNDLLFKVGGKMFAVTPLEPAQ
                     VCLSFKASPENFAELTERPDIIPAPYLARAQWVALQTRDALPADELARLLRDSYDMVF
                     AKLPKKTREAVSSSKPSARKARSKKPEKKKHRRSR"
     gene            complement(2693..>3082)
                     /locus_tag="VGR03_07115"
     CDS             complement(2693..>3082)
                     /locus_tag="VGR03_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994081.1"
                     /translation="GGSPTLVRKTFAGVGAGSAGLLLLGCALAGPRASVILLLLAFAA
                     GGMCGSNIWAITQTLAGPKMAGRWTGFQNFLGNLAGVIAPAVTGFVADYTGHFLVAFV
                     IMAIVGLLASLSYIFVIGPVKEIAWEA"
BASE COUNT          637 a          909 c          896 g          640 t
ORIGIN      
        1 gcgctggccc gcgtagattt cagttaccgg ttacggtggc acatctgcga ctggcccgaa
       61 taccctacga agtggagttc gtggacctcg aagactcccg gtaacaccaa ttggccccaa
      121 gacgtcactt gcagcggaat cacaccgtgg ggcgcgcgta caggtatcga ttgcatcaac
      181 cctctgccgg agactcgatg ttctcgaaat cagcctccgc ggccactccg caacttattg
      241 aatttaaaat acttaggaat ggagcgtaag ccggggacag tatcgaccta gagacagtac
      301 taggcctagg gtacggaaaa gagggggctc cggcatagga caggggtccg gtctaaattg
      361 aacctggtgc ttcatggtgt ggtaaagtct ccggcgccat actcgatcca ggttgaggcg
      421 ctgtgctaga cacctcggga cgaaatatct gagctgaaaa ggagaagtta ggaaaatgtg
      481 taaacaccaa aagggattct cgttgatcga actgctgatc gtggttgcga tcattctgat
      541 tatcgctgcc atcgccattc caaacctgct ccggtcgcgt atgtctgcaa acgaggcttc
      601 cgctgttggt tctctgcgca ccatcaatac cgcggaaata gcctattcca cgacgtaccc
      661 ggaccttggg ttcgcggggc tcaccgccct tggcggtgtg gcaccttgca ctgtcgcaac
      721 gcctgcgtcg gcttgcttga tcgacgacgt cttgtcgtcc gcggcggcgg ttaagagcgg
      781 ttatggtttc accgttacga acctgactgg ggcaccggtg gttgtaacct atactgccac
      841 ggctctcccg gtcgcagttg gccagagcgg ccagcgcgcg ttctgttccg accagtctgg
      901 cgtcgtccgc tacaacgaca ctggcaccgc ctgcgacgcg accgcggacc cggccctcta
      961 agaagctcaa ttggcgacct tagttccggt cgcagccgga gaatgttccg gcgggacgga
     1021 tcgaaaagcc cactcactcc gacaggagaa gtaggctttt tcatttcact cgcagaccct
     1081 cacacccaag gatccggtcg gttacacatt ctgtgacttt tactgtgaag ctggaaccca
     1141 ggcaaggcca tctacgcctg gagtgagatc gatcgtctgc tgcattttcc aggagtgcag
     1201 gtcgagcacc gcgatcttcc ccgcgccggt cccgctcacg taggcgatat ccccgccggg
     1261 tcgaatcagg atttccgacg atttctccgg aacctccagc gagcgcacca ccttcagcgt
     1321 ttgcaaatca acgacgtgga gacgattggc tgcaaggctg actgcgagca accagcgccc
     1381 atcgggtgtc ggcgccgagg catacgccac gtccggcaat gcgatccagt tcgtgattct
     1441 gttcgtggcc gtatcaataa cggcaatgcg cggggcatcc tggtcttgag tgaagatgcg
     1501 gcggccatcc ggtgaaatgg aaatacgctg gacacttttg gccacgggaa tcgtcgtgat
     1561 gagattgcgc ttcgccagat ccagcacgct cacgctgccg gcgctcacgt tggccgtgta
     1621 cgcgcggtgt ccgtcgcgcg acagaaccag catgtgcgat tgcggctcgc ccgtgggaat
     1681 ttcagcgagc accttgtttg tggatggatc gatgacgtcg acggcattcg caagctcggc
     1741 ggtgacgtac agcagcccgt ccggcccgaa ttcggcgcgg tgcgggcgga gcggtttgcc
     1801 catgtcgatc gtggcggcga gtttgtgctc ctgcagatcg acgatgtcaa tcgtgctgcc
     1861 gtccgtccct ggtcttccga cgccggagtt tccgtagatc ggaacgtaag ccaagcggcc
     1921 gtctttcgac acgatgactt catggccgtt cactcccaca gcaatttttg ccagttcgcg
     1981 gcgattgtcg ggattgacga ccagtagggt gtgctccttc tgattcgcca cgaccagcag
     2041 accacctgcc ggcggcgctg gctttgcgtc tcggagcagt cctgaagtga caagcatcat
     2101 ggaaacaagg agcaatgaca ggcgtgtaag cacggaccac ctcgatggaa catttatctc
     2161 gttcgaaagc gcggaaactc agcgcagtat atcttttccc aaagtcagcg gctacgtctg
     2221 tgtttctttt tttccggttt tttgcttcga gccttgcgcg ccgagggctt tgagctggag
     2281 accgcctcgc gcgttttctt cggaagcttc gcgaaaacca tgtcatagga atcgcgcagc
     2341 aagcgcgcga gttcgtccgc cggcaatgcg tccctggttt ggagcgctac ccattgcgct
     2401 cgcgccaggt acggcgcggg aataatgtcg ggccgctccg tgagttccgc aaaattttcc
     2461 ggcgacgcct taaacgagag gcacacctga gccggctcca gcggcgtcac ggcgaacatc
     2521 tttccgccga ctttgaatag caggtcgttg ccccactgga tctgttccgc ggcgcccgga
     2581 aaggaaaggc aaacctcgcg cagccaatcg acagtcatga gcaagatttt aggataaaga
     2641 gggtgctggc ctcaatgaat gaggcgatga attcagcgac cccgctcgcg ggtcatgctt
     2701 cccaggcgat ttccttgacc gggccgataa caaatatgta agaaagagag gcaagcagcc
     2761 cgacgatcgc cataattacg aacgccacga ggaaatgtcc ggtgtagtcg gcgacgaatc
     2821 cggtcactgc cggtgcaatg acgcccgcaa gatttcccag gaaattctga aagcccgtcc
     2881 accggcccgc cattttcggc ccggccagcg tttgcgtgat cgcccagata ttcgacccgc
     2941 acatgccgcc ggctgcgaac gccagtagta gcagaatcac tgaagccctc ggaccggcta
     3001 gggcgcagcc aaggaggagt agcccggcac tccccgctcc tacaccagca aaagtcttgc
     3061 gaaccagcgt gggcgatcca cc
//
LOCUS       DASYVO010000216         7168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_245682, whole genome shotgun sequence.
ACCESSION   DASYVO010000216 DASYVO010000000
VERSION     DASYVO010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7168
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245682"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VGR03_07120"
     CDS             <1..348
                     /locus_tag="VGR03_07120"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1994082.1"
                     /translation="KTRELSDSAMEILLRYPWPGNVRELRNLVERLVIVCPQPRIEPH
                     HLPPELFRGVAESPQHPYSTLHEARSAYEREFILQKLQEHRWNMTQTATALGLERSHL
                     YRKMKSLGIAAPD"
     gene            588..1199
                     /locus_tag="VGR03_07125"
     CDS             588..1199
                     /locus_tag="VGR03_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994083.1"
                     /translation="MKKMTLLFIALLTVFALTSQSQTKTVETGHASKPPDKSRTFFPH
                     NWVRGYTDFAVAPSHNEPDLGRCSFPQPASAGGAASPCTAYARYLLSGYIEFQPLGRT
                     PARHFFLFFEPKFSFGRNIPQLTYTASMLPMAFDRSIGIGFQLPKNFELRATQHQVDW
                     LGRYSKTLGTADLHTNGPYGLYATFGVRWSFGGYGHQGAPSDY"
     gene            complement(1276..2226)
                     /gene="era"
                     /locus_tag="VGR03_07130"
     CDS             complement(1276..2226)
                     /gene="era"
                     /locus_tag="VGR03_07130"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV1994084.1"
                     /translation="MERSEKTEKKFFRSGFVAIVGRPNAGKSTLVNRLVGQKIAIVTS
                     KPQTTRNRIQGIVTKPQGQIVFIDTPGVHEANSALGRQMMQEVAAALEGIDVLLLMVD
                     ASSPHAHVDELLLEKAKRFRGKTILALNKVDGLPKPKLLPLIESFAKAFEFSAIVPIS
                     ALRGAGCEELLDEILKQLPEGEPYFPEDQVTDQPERFLAAEVIREKAIQLMYHEVPYA
                     LAVVVEKYEETPKLLRIEALMNVERDSQKKILIGHKGEMLKKIGTEARKELEVILGSK
                     IYLGLFVKVAPDWRENPQKVRELDWRYQLEGLAEELDDKE"
     gene            complement(2345..3631)
                     /locus_tag="VGR03_07135"
     CDS             complement(2345..3631)
                     /locus_tag="VGR03_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEV1994085.1"
                     /translation="MNLSWLYALSVVLVAFTLPIFSYLTLIYRELGRMTTGRVHEHLE
                     IFEAEIEPKLKINRRSGGRTFRILGHFWLAFLVLETTRGVVYFVPGTWESFVQFFVFL
                     VLEVVIAMHFIPDMLLYRTTGRWLLPLLPLIRGAMWFVWPVRIFLEGAESLARISEHE
                     VEKTEEQRTEEGIEALVEAAEEEGIIEPEQGDLIEQVVEFSDKRVREVMTPRPDIVAL
                     PADATLEDLHAKVVETKLNKIPVYEKSLDDIFGVVYSQDLLQIADQDLLKRKVRELVK
                     PVLFIPETKVGSELLREMRQKGQPLAVVIDEHGTVAGIATVEDLVEEIVGESGNDGKP
                     PAPDVVREPDGGLVMRGSMPIGDVEELLGVKFGDKTDEAVTTLAGLLSHVSGKVPAPG
                     DKIDLADHRFEVLEANQRKVLRVRIRKQRMAAPAKP"
     gene            complement(3631..4074)
                     /gene="ybeY"
                     /locus_tag="VGR03_07140"
     CDS             complement(3631..4074)
                     /gene="ybeY"
                     /locus_tag="VGR03_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV1994086.1"
                     /translation="MILNRQRAVRLARGPLESFLRRVQEEVGFQRAGVTVCLVSDAEI
                     ARLNETFRKKKGRTDVLSFPTVARRRPVRLGSRAVKTGEYLGDIAISPATARRYAKKN
                     GRKLSSELQVLILHGVLHLLGYDHEIDRGEMERIERKLRKRLGLA"
     gene            complement(4071..5108)
                     /locus_tag="VGR03_07145"
     CDS             complement(4071..5108)
                     /locus_tag="VGR03_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV1994087.1"
                     /translation="MRRTVHISGQIEPFFGTLDENVRLLEDLLRVRTHLHDTRLTIEG
                     EQDSVDQAVKIVEEYNHLTRHGKTLTAAEVKSLMRVATEEPHESPRGMFEHSRVRSFG
                     KKQVTPKNASQKRYMEEIEKHDMMFGIGPGGTGKTYIAVAMAVSALLTKQVNRIILAR
                     PAVEAGERLGFLPGTLQQKIDPYMRPLYDALYDMLDADKLERFLEKGIIEVAPLAFMR
                     GRTLNDSFVILDEAQNTTSEQMKMFLTRLGFNSKAVITGDVTQIDLPQGKKSGLIEAL
                     EVCEKIEGIGVVQFGEKDVVRHNLVQQIIRAYEEYDVAHPQRGGNTANGKLGMPTRES
                     GKEREKETQQG"
     gene            complement(5319..6548)
                     /locus_tag="VGR03_07150"
     CDS             complement(5319..6548)
                     /locus_tag="VGR03_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEV1994088.1"
                     /translation="MAARLVVTLALCANGLFAQAQTQAPAKPAGDGGELAEAKARLEA
                     QEKRLNDWPDLAHYRDANAKVAAPAEGEQRVVFMGDSITDMWVLPRFGGFFPGKPYIG
                     RGISGQTTPQMLLRFRADVIALRPMVVVILAGTNDIAGNTGPITLEETEGNLASMAEL
                     ARANGIRVVLSSVMPVSNYGRDGVGKPIDMRIKRPPEKILELNAWIRKYAAVNDHVYL
                     DYFSATVDEHGLLKKELSEDGLHPNALGYAVMVPLAEKAIQSALREIPASSFGFQAPV
                     PVAKQTEQEMVSELYGLYYVDLGEQGSGETALVISPDGKPIPPKENDPDAPTEPGFHT
                     NGQHFKFAWSQLTLQDFSFRTVNVNGSEYSFQGQFGREQVDVIPDVPYLEGVLTEKRD
                     SNIVRAKKVHFGHAVIL"
BASE COUNT         1422 a         2296 c         1756 g         1694 t
ORIGIN      
        1 aaaacgcgcg aactcagcga ctctgcaatg gaaattcttc tgcgttatcc atggccggga
       61 aacgttcgag aactgcgaaa cctcgtcgag cggctggtga tcgtgtgtcc tcagccgcgg
      121 attgagcccc atcacctacc gcccgaactt ttccgcggcg tcgcggaaag cccccagcat
      181 ccgtattcga cgctgcatga agcgcgcagc gcctatgagc gggaattcat cctgcagaaa
      241 ttgcaggagc accgctggaa catgacacaa acggccacgg ctctcggcct ggaacgcagc
      301 cacctctacc gcaaaatgaa gtctctggga atcgctgccc ccgactaaaa ctatccgaac
      361 cagcggagcc gccttccgcc gcagtccagt tctctttttc ccgcatgaca cgcaattcgt
      421 gtagaatccg ccgaaccggt gtctcacttt tcacaacact gactggatca gcggttctca
      481 cgcggacgct ctcggtccac cgccgcttca ttcacagaaa ttctgacggg aatacacacg
      541 ccggccaatt gcttcgataa aattcgtcga aaatgcgagg aactccgttg aagaaaatga
      601 ctctcctctt cattgcgctg ctgaccgtct tcgcccttac ctcgcagtcg cagaccaaga
      661 ctgtcgaaac cggccatgca tctaaacccc cggacaaatc gcggacattt ttcccgcaca
      721 actgggtccg cggttatacg gatttcgccg tggcgccgtc gcacaacgaa ccggaccttg
      781 gccgatgttc gtttcctcag ccagccagtg cgggcggcgc ggcttccccc tgcaccgcct
      841 acgcgcgcta tctactcagc ggatacattg agtttcaacc gctgggccgc actcccgcgc
      901 gccatttctt tctattcttc gaacccaaat tcagctttgg aagaaatatc ccccagctca
      961 cctacaccgc atccatgttg ccgatggctt tcgatcgttc gattggaatc ggtttccaat
     1021 tgccgaaaaa cttcgaattg cgcgcaacgc aacatcaggt ggattggctc gggcgttaca
     1081 gcaagaccct gggcacggcg gaccttcaca ccaacggccc ttacggtctt tatgccactt
     1141 ttggcgtgcg ctggtctttc ggaggatatg ggcaccaggg agccccgtcg gactattaat
     1201 cacgcaatct aagacaccgg gacttcttca cgatggcgaa acctgagctc gctatccttc
     1261 gtggacaagt gtttctcatt ctttgtcgtc cagctcctcg gcaagccctt ccagttggta
     1321 acgccagtcg agttcacgca ccttttgcgg attttcccgc caatccggtg cgactttcac
     1381 gaacagtccc aggtagatct tgctgccgag gataacctct agttctttgc gggcttccgt
     1441 tccgatcttc ttcagcatct cgcccttgtg gccgattagt atttttttct gcgaatcgcg
     1501 ctccacgttc atgagtgctt cgatgcgcag tagcttgggc gtctcttcgt atttttcaac
     1561 gaccaccgct agcgcgtacg gcacttcgtg gtacatcaac tgaatggcct tctcgcgaat
     1621 gacctcggcg gccaggaatc gttccggctg gtccgtcacc tgatcttccg gaaagtatgg
     1681 ctcgccctcc ggcagctgct tcagaatctc gtccagcagc tcttcgcacc ccgcgccccg
     1741 caatgcggaa atcggcacga tggcgctgaa ttcaaaagcc tttgcaaacg attcgatcag
     1801 cggcagaagc ttgggcttgg gcaatccatc taccttgttc agcgccagaa tcgttttccc
     1861 gcgaaaccgc ttggccttct ccagcagcaa ttcatccaca tgtgcatgcg gagagctcgc
     1921 gtccaccatg agcaacaaaa cgtcaattcc ttccagtgct gccgccactt cctgcatcat
     1981 ctgccgcccg agcgccgaat tcgcttcgtg cactcccggc gtgtcgatga acacgatctg
     2041 cccttgcggc ttggtcacaa ttccttgaat gcggttgcgc gtggtctgcg gtttggaggt
     2101 aacgatcgcg atcttctgcc cgaccaggcg attcacaagc gtggacttac ccgcattggg
     2161 ccgcccgacg atggccacaa atccggagcg aaaaaacttc ttctccgttt tttcacttct
     2221 ttccattctt ggctcttctc tgcaagtctc tgcgctttct gcgtctctgc gtttgaatct
     2281 tcttcctgct ggattgccga acgacgggaa tctcgagtgc tcgacggccc cgctcactgt
     2341 atgcctacgg cttcgccgga gccgccatcc tctgcttgcg aatccgcacg cgcagcactt
     2401 ttctctggtt tgcttccagg acttcgaagc gatgatcggc caaatcgatc ttgtcaccgg
     2461 gcgccggcac tttgccggaa acatgactca gcaaacctgc aagagtggtc accgcctcat
     2521 ccgtcttgtc gccaaacttc acgccaagta attcctcgac atcgccgatg ggcatgctgc
     2581 cgcgcatcac caggccgcca tcaggttcgc gcacgacgtc cggcgcggga ggctttccgt
     2641 cgtttccgct ttcgccgacg atctcttcca ccagatcttc cacggtcgcg atgcccgcca
     2701 ccgttccatg ctcatcgata accaccgcca gcggctgccc cttctggcgc atttcgcgca
     2761 gcaactccga accaaccttt gtttccggta tgaagagaac cggtttgacc agttcacgca
     2821 ccttgcgctt cagcaaatcc tggtccgcga tttgcagcag gtcctgcgag tagacgacgc
     2881 cgaaaatatc gtcgagcgat ttctcataca cgggaatctt gttcaatttc gtctcgacaa
     2941 ccttggcgtg gaggtcttcc agcgtggcat ccgcaggcag ggccacaatg tcgggccgcg
     3001 gggtcatcac ttcgcgcacg cgtttgtcgc tgaattcaac gacttgctcg atcaagtctc
     3061 cctgctccgg ctcgatgatg ccttcttctt ccgcggcttc caccagcgct tcgattccct
     3121 cttccgtgcg ctgctcctcg gtcttctcga cctcgtgctc cgaaattcgt gcgagcgatt
     3181 ccgcgccttc cagaaatatg cgcaccggcc agacgaacca catcgcgccg cgaattaacg
     3241 gcaagagtgg aagcagccag cgcccggtgg tgcggtacag caacatgtcc ggaataaaat
     3301 gcatggcgat gacgacttcg agcactagaa atacgaagaa ctgaacgaaa gactcccagg
     3361 tacccgggac gaagtagacc actcctcgcg tcgtctccag caccaggaac gccagccaga
     3421 agtgcccgag gattcgaaag gtgcgcccgc cgctgcggcg gttgatcttc agcttcggct
     3481 cgatctccgc ctcaaaaatt tccagatgtt cgtggacccg gcccgtggtc atgcggccca
     3541 gttcgcggta aatcaaagtc aggtaggaga aaataggcag tgtaaaagct accagcacga
     3601 cgctcagcgc atagagccag cttaaattca tcacgccagt ccgagccgtt ttcgcagctt
     3661 ccgctcgatc cgttccattt caccgcgatc aatttcgtgg tcgtagccca gcagatgcaa
     3721 gacaccgtgc agaatcagca cttgaagttc gctcgaaagc ttccgcccgt tttttttcgc
     3781 atagcgccgc gccgtcgcgg gagagatggc aatgtcgccc aggtattcgc cggtctttac
     3841 tgctcttgag cctagtcgca ccggtctccg ccgtgccacg gttggaaaag aaagcacgtc
     3901 ggtgcggcct tttttcttgc gaaatgtctc attcaaccgg gcgatctccg cgtcgcttac
     3961 caaacagacg gtgacgcctg ctctttggaa tcccacttcc tcttgtacac ggcgtagaaa
     4021 cgattcgagt gggccccgcg ccaggcgcac cgctcgctgg cggttcagaa tcatccttgc
     4081 tgtgtttctt tttctcgttc tttaccggat tctctcgtcg gcattcccaa tttgccattc
     4141 gcagtgtttc cgccgcgttg cgggtgagct acatcatact cttcataggc gcggatgatc
     4201 tgttgcacca ggttgtgtcg caccacgtcc ttctccccga actgcaccac gccgatcccc
     4261 tcgatcttct cgcaaacttc cagcgcttcg atcaagccgg attttttccc ttgtggcagg
     4321 tcgatctgcg tcacgtcgcc ggtaatcacc gccttggagt tgaaccccag tcgcgtcagg
     4381 aacattttca tttgctcgct ggtggtgttc tgcgcttcgt ccagaatcac gaacgaatca
     4441 ttcagcgtgc ggccacgcat gaacgccagc ggcgcaactt cgatgattcc cttttccaaa
     4501 aaacgctcca gcttgtccgc gtccagcatg tcgtacagcg cgtcgtacag cggccgcatg
     4561 tacggatcga tcttctgctg cagcgtgccc ggcaaaaatc ccagccgctc acccgcttcc
     4621 accgcgggcc gcgccaggat gatgcggttc acttgcttcg tcagcagcgc gctgaccgcc
     4681 atggccacgg cgatgtacgt ctttccggtg ccgcccggac cgatgccgaa catcatgtcg
     4741 tgcttctcga tctcttccat gtagcgcttc tggctggcgt tcttcggcgt gacctgtttc
     4801 ttgccgaacg aacgcacgcg gctgtgctca aacattccgc gcggcgattc gtgcggttcc
     4861 tcggtcgcca cgcgcatcag cgatttcact tccgccgccg taagggtctt tccatgccgc
     4921 gtcaggtggt tgtactcctc cacgatcttc acggcctggt cgacgctgtc ctgctcgcct
     4981 tcgatggtca gccgcgtgtc gtgcagatgc gtgcgcaccc gcagcaaatc ctcgagcagg
     5041 cgcacatttt catccagcgt gccgaagaag ggttcgattt gtccggaaat gtgaacggta
     5101 cgtcgcatcc aattggaagg tttttctgct acgggaaccg ggtggccgat gggattcctc
     5161 ctgtttgtgc gtgacccctt cgcggttcac ttacggagaa actaattaca ccctagcgca
     5221 gccttccccc gcggtcaagt tacgccttca tgaacgcatc gccacggact ctttggcgag
     5281 gtggaaacaa gcggcatttg agcttcttgg ccggtgcact aaaggatgac cgcatgccca
     5341 aagtgaactt tcttcgctcg cacaatatta gagtctcgct tctctgttag caccccctca
     5401 agatagggca cgtcaggtat gacgtcgacc tgctcgcgcc caaactggcc ctggaacgag
     5461 tattcgctcc cgtttacatt caccgtgcgg aacgaaaagt cctgcagggt taactgggac
     5521 cacgcaaact taaaatgctg tccattggtg tggaatcctg gctccgttgg cgcgtctgga
     5581 tcgttctcct tcggcggaat cggctttcca tccggactga taaccagcgc ggtttcgccg
     5641 cttccttgct cgcccaagtc cacatagtag aggccgtaaa gttcgctaac catctcttgc
     5701 tctgtttgct ttgcgaccgg aacaggagct tggaagccaa agctcgatgc tgggatttcg
     5761 cgcagcgctg attgaattgc tttctctgcc aaaggcacca tcaccgcata cccgagtgcg
     5821 ttcggatgca acccatcctc gctgagttct ttcttgagca agccgtgttc atccacggtc
     5881 gcggaaaaat aatcgaggta cacgtggtcg ttcaccgcgg cgtacttcct gatccacgcg
     5941 ttcaattcaa gaatcttctc cggcggcctc ttgatgcgca tatcaatcgg ctttcctacg
     6001 ccgtcgcgcc cataattgct gaccggcatc acggaagaca gcaccacgcg aattccgttc
     6061 gcgcgcgcca attccgccat cgaagcaaga ttcccttccg tttcctccag cgtgatgggg
     6121 ccagtattcc ccgcgatgtc gttcgttccc gcgagaatca ccacaaccat aggccgcagc
     6181 gcaatcacat ccgcacgaaa acgcagcagc atctgtggcg tcgtctgccc gcttattccc
     6241 cgcccgatgt acggcttccc cggaaaaaat cccccgaacc gtggcagcac ccacatatcc
     6301 gtaatcgaat cgcccatgaa caccacgcgc tgctcgccct ctgcaggcgc cgctactttc
     6361 gcattggcgt cccgatagtg cgccaggtcc ggccaatcat tcagccgctt ttcctgagcc
     6421 tccagcctcg ccttcgcttc cgccaattcc ccgccatctc ccgccggctt cgccggcgct
     6481 tgcgtctggg cctgcgcaaa tagcccgttg gcacacaagg ccagcgtcac aacaagtcga
     6541 gcagccatct tgaaagcagc ctccatagat ctcctcttct tcctgcgaag aacgaacaac
     6601 tggataccag gcactagtgt ctggctaaag gatgagcata tacaattggt tacatctacc
     6661 caatcgctcg ctccacaagt tttccttgac ggcagtcaag ttttcgtcta atatatcgct
     6721 caggtaaaca tccgtcctgg agtgcggcag cttgctgccg cttttctttt ggtgccgacg
     6781 ccgtttgacc cgcaatcctt tagcccccga gccccacggt cgcaagaatc gcccgcactc
     6841 ttgcaccatt ctagtgcaac gtaaccacct ttagaatcaa cacttatgga ggccatggaa
     6901 gtattcattc taaacaactt aaaaccgctc tgttgacatc ccgttttccc ccaccttccg
     6961 ttacagatgt catcccgaat ccccgcggtt ttggcggggt gagggatctg tttttcgctt
     7021 tcgggtgccc tcttcgcgag gatctcaaca gagccacttt aactcccttt agaatcaaca
     7081 cttatgaaaa caagccaaat ggccacattt tgcacaattt tggtgcaacg taactatctt
     7141 tagaatcaac acttatagaa gtgttcat
//
LOCUS       DASYVO010000217         5624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_229701, whole genome shotgun sequence.
ACCESSION   DASYVO010000217 DASYVO010000000
VERSION     DASYVO010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229701"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VGR03_07155"
     CDS             complement(<1..840)
                     /locus_tag="VGR03_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEV1994089.1"
                     /translation="MRQRLHIAAGTLLLAAVLVGCEDAGKKPVQARVPALAPASAAAR
                     QAPVELALLPIVNPPHRHYVWLLPPVPAGKDYLIQKVQEKFASGEQNFKAGHLEAARK
                     DFDDAVDWMLESGYDPNGDPKLSELFHRVVDTVYAYELQAFRAGDSFSEAPAVPAAID
                     EVAEMTFPVDPRLKERAEEAAKNISHDLPLTVNDQVLSFVNFFQTPRGRAIVETGLRR
                     AGRYREMISRVLREEGMPQDLIYLAQAESAFQPLALSRAGARGIWQFVAYRGQEYGLR
                     HTWW"
     gene            1182..1388
                     /locus_tag="VGR03_07160"
     CDS             1182..1388
                     /locus_tag="VGR03_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEV1994090.1"
                     /translation="MSKEQGTVKWFNASKGFGFIQRQSGEDVFVHFSAIVADGYKTLN
                     DGQAVEFEVSKGPKGLQASNVSPL"
     gene            complement(1619..2092)
                     /locus_tag="VGR03_07165"
     CDS             complement(1619..2092)
                     /locus_tag="VGR03_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994091.1"
                     /translation="MKRFAVLTMTLLLVTLAVSVAAKAGDQHSGTWKMNPAKSTYSPG
                     PAAKSVTVRIDSDENNFTVNAEGTGGDGNPTHVEYSAKFDGKDYTITGVPGADTVSVK
                     RIDTNTVESTIKYAGQTVMTVRSTVSKDGNTRTSVFKGKDAQGRDVNNVVVYDKQ"
     gene            2146..2319
                     /locus_tag="VGR03_07170"
     CDS             2146..2319
                     /locus_tag="VGR03_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994092.1"
                     /translation="MDAISQKVQWILIVLGFVGARYTCPDLVGIVPLLAVCHPGRSEG
                     IVATLVESLIDAQ"
     gene            complement(2323..2796)
                     /locus_tag="VGR03_07175"
     CDS             complement(2323..2796)
                     /locus_tag="VGR03_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994093.1"
                     /translation="MGVAVEIHGFAERRIFFASFEEVALSGLERDAVALTDGFDDGAR
                     VDAFVDVEGNGGDFEGNVLFLAGPDELRVEVRIVFVGFVRSDGRIGFLSDEADGGLLT
                     RVLPLWSYCSMGRFLDLDSVESFVLVLCPFLGRATKLPRRNMRMLFARDFTRSSP"
     gene            2773..5109
                     /locus_tag="VGR03_07180"
     CDS             2773..5109
                     /locus_tag="VGR03_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEV1994094.1"
                     /translation="MDFYRHAHGWTNRLIAGDSLLVMNSLLEKEGLAGQVQMVYIDPP
                     YGIRYGSNFQPFVNRRDVKDGKDEDLTQEPEMVRAFRDTWELGIHSYLTYLRDRLLLA
                     RDLLHESGSCFVQISDENVHFVRCLMDDIFGSANFMGLIFVSKTSGFGQTLSIASVGD
                     YLVWYSKGKELCKYRQLYVDKKEDSPQAKDYRFALLANGQSRALSLAEISGTTKLPPN
                     ERRFSLTATTAQGATDAGGFSVEFNGKIFNISGDQHWKTSENGMKRLIEMGRIHAREN
                     SLRYIRFLDDFPASPITNNWVDLRWGFDAGEKRYVVETNPGIVARCTLMTTDPGDIVF
                     DPTCGSGTTVFVAEQWGRRWITCDTSRVATTLAKQRLMGATFDYYKLAHPNEGVGSGF
                     EYKKVPHVTLKSIANNEPPGEETLYDQPLIDNSRARISGPFTVEAVPAPNVKSLDDVS
                     GSCHPESPRSLRGEGSAVSGSASPSATPPQPADASIARSGPTLRQSDWRDELLKTGLR
                     GKGGQMINFSRVEPLGGTRWLHADAETKGVKPERVVISFGPDYAPLVTTQVEMAWEEA
                     RTLHPKPEILVFAAFQFDPEAAKDIDDLTKEKTGMTFLTAQMNADLLTDDLKKKRSSS
                     QSFWLVGRPDVDIRRAKSSDSSRLSSSASSLSSRTNVRDPLSSGLYIAEVRGFDYYNT
                     RTGTIESGDTKKIAMWLLDTDYDGRSLYPRQVFFPLADEDEGWAKLARNLKAEIDPDL
                     IEHYRGTISLPFALGDHKRVAVKVIDDRGIESLKVVQL"
     gene            5237..>5624
                     /locus_tag="VGR03_07185"
     CDS             5237..>5624
                     /locus_tag="VGR03_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV1994095.1"
                     /translation="MNHNYAIYILASRSRSLYTGVTDNLERRMIEHRQGLVPGFTTRY
                     RVFRLVHFESFGHIQDAIAREKEIKGWRREKKISLIEQHNPTWEDLAAQLPHHYQSLQ
                     HQNQIPQRSPNTGDHVRDDNLHVSFRD"
BASE COUNT         1215 a         1898 c         1350 g         1161 t
ORIGIN      
        1 ccaccaggtg tgccgcaggc cgtattcctg cccgcggtag gccacgaatt gccagatccc
       61 tcgcgcaccg gcgcgcgaaa gcgccagcgg ctggaaggcg ctttcggctt gcgccaggta
      121 aatcagatcc tgcggcatgc cttcttcccg caaaacgcgg gaaatcatct cgcgataacg
      181 cccggcgcgg cgcagtccag tttccacaat ggcgcggccc cgcggcgttt ggaaaaaatt
      241 cacaaacgaa agcacctggt cgttcacggt cagcggcagg tcgtgggaaa tatttttggc
      301 cgcttcctcg gcccgctctt tcagccgcgg atcgacagga aaagtcatct ccgccacttc
      361 gtcaatcgcc gccggaaccg cgggagcttc gctgaagctg tctccggctc ggaaggcctg
      421 cagttcgtag gcatacaccg tgtccaccac gcggtgaaac agctcgctca gtttgggatc
      481 gccgttgggg tcgtagccgc tctccagcat ccagtcgacg gcgtcgtcaa aatccttgcg
      541 cgcggcctcc aggtgaccgg ctttgaaatt ttgctcaccc gaggcgaatt tctcctgcac
      601 cttctggatt aagtaatcct tcccggccgg aaccgggggc aacagccaga cgtaatgtcg
      661 gtgcggcgga ttcacgatcg gcaaaagcgc aagctccacc ggagcctgcc gcgcggccgc
      721 cgaagccggc gccagcgccg gaactcgcgc ctgcacgggc ttttttcccg cgtcttcgca
      781 gcccaccaga accgccgcca atagcagggt acccgccgcg atgtgcagcc tttgcctcat
      841 ctctccatgg taagtagcgc ctcaccgcaa ggtcaacgct ttccggcggg cagggggtga
      901 cgctctcagt acacgccgca ccttcaccag aatccccttg catcgtcccc cgttccgtgg
      961 tactctgttt ttgcgtttgt tgctcttgta tcgcctttgg gtagtaaagc ggtcgcaagg
     1021 tcactcttgc ggcctttttt ttgttcggtg gaccgctttg cagtcatccc gaacgaagct
     1081 tgaagacggc cgcggctctg ttgaggggct cgggacttga ggcatcagat agctcaatgt
     1141 agtgctcagc cggtccccgg ctgacagaaa agagtaaagc agtgagtaaa gaacaaggta
     1201 cagtgaagtg gttcaatgcc agcaagggct tcggcttcat ccagcgccaa agcggcgagg
     1261 acgttttcgt ccatttctcc gcgatcgtgg ctgatggcta taagactttg aacgatggcc
     1321 aggcggttga attcgaagtt tccaagggtc ccaagggcct gcaggcctcg aacgtttcgc
     1381 ccctctaatg cacggctgat cccggcctaa tcgggcttaa cactcgatac cgccggtttc
     1441 ggcttcactt cacaaaaatt cagaacgggc gtcccgatgc aaatcgaggc gcccgttctg
     1501 ctttttgccc tctaacactc cgcgacctgt ccctttttcc gatgtcatcc cgagcgcagc
     1561 cgagggatct gcttttgctc tttgtagccg cggccttcta atgccgacat cccccgcctt
     1621 actgtttgtc gtacaccacg acgttgttta cgtcacgccc ctgcgcgtcc ttgcccttga
     1681 agaccgacgt ccgcgtattt ccgtctttgg agaccgtcga tctcacggtc atcaccgtct
     1741 ggccggcata ctttattgtg gactccaccg tgttggtgtc gatgcgcttc accgataccg
     1801 tatcggcgcc gggcaccccg gtaatcgtgt aatccttgcc gtcgaatttt gcgctgtatt
     1861 ccacgtgcgt cggatttcca tcgcccccgg ttccctcggc attcaccgtg aagttgttct
     1921 cgtcagagtc gattctcacg gtgacactct tcgccgctgg cccaggactg tacgttgact
     1981 ttgcggggtt catcttccag gtaccagaat gctgatcgcc agccttggct gcaacgctta
     2041 cagccagagt cacaagcagc agtgtcatcg tcaaaacggc aaacctcttc attcgcataa
     2101 tccattctcc ttggggaata tgagcggtgg gcctgcctgg acgttatgga cgcaatttcg
     2161 cagaaagtcc agtggattct tatcgttctc ggttttgtag gggcacgata tacctgtccc
     2221 gacctggtcg ggatcgtgcc ccttcttgct gtgtgtcatc ccggccgcag cgaagggatc
     2281 gtggctaccc tcgtcgagtc cttgatcgac gcccagtagc actcacgggg acgaacgagt
     2341 aaaatcacgc gcgaacagca tacgcatatt cctgcgaggc aatttcgtgg cccgacccaa
     2401 aaacggacac aagaccagca cgaaagactc cacggaatcc aaatccaaaa agcgtcccat
     2461 cgagcagtac gaccacaaag gcaaaacgcg cgtcaacaat cccccgtcgg cctcgtcact
     2521 caaaaaaccg atccgcccgt cacttcgcac aaaacctacg aatacgatcc gcacctcgac
     2581 ccgcaactcg tctgggccgg caagaaagag cacatttcct tcgaagtccc caccgtttcc
     2641 ctccacgtcc acgaacgcat cgacccgcgc accatcatcg aagccgtccg taagcgcaac
     2701 ggcatcccgc tcaagcccgc tcagggctac ctcttcgaaa ctcgcgaaga aaatcctcct
     2761 ctccgcgaag ccgtggattt ctaccgccac gcccacggct ggaccaaccg tctcatcgcc
     2821 ggcgattcac tcctcgtcat gaactccctc ctcgaaaaag aaggcctggc cggccaggtc
     2881 cagatggttt acatcgaccc gccctacggc atccgctacg gctccaattt ccagcccttc
     2941 gtcaatcgcc gcgatgtcaa ggacggcaag gacgaagacc tcacccagga acccgaaatg
     3001 gtccgtgcct tccgcgacac ctgggagctc ggcatccact cctacctcac ctatctccgc
     3061 gaccgtctcc tcctcgcccg cgatctcctc cacgaatccg gctcctgctt cgtccaaatc
     3121 tccgacgaaa atgttcattt cgtgcgttgt cttatggacg atattttcgg aagtgcaaat
     3181 ttcatgggac tcatttttgt ttccaaaact agcggattcg gacaaacgct ctcgattgcc
     3241 tcggtgggag actatttggt ctggtattcg aagggaaaag agctatgcaa ataccgccag
     3301 ctctatgtcg acaaaaaaga agattcaccg caggcaaagg actaccgctt tgccttgctc
     3361 gctaatggtc agtctcgggc actttcgctc gccgaaatat cgggaactac aaaattgccg
     3421 cccaatgagc gacgcttcag cttaacggca accactgcgc agggggccac cgacgccggc
     3481 ggattttccg tggagtttaa cggcaagata ttcaacatta gcggtgacca acactggaaa
     3541 acctcggaaa acggcatgaa gaggctgatc gagatggggc gaatccacgc aagagaaaat
     3601 tcgttgcgct acatcagatt ccttgacgat ttccctgcct cgcctattac caacaattgg
     3661 gtcgatctgc gatgggggtt cgacgctgga gaaaaacgtt atgtggtcga gacaaatccg
     3721 ggaattgttg cccgctgtac tctaatgacc accgatcctg gcgacatcgt tttcgatcca
     3781 acatgcggct ccggcacaac cgtcttcgtc gccgaacaat ggggccgccg ctggatcacc
     3841 tgcgatacct cccgcgtcgc caccaccctc gccaaacaac gcctcatggg cgccaccttc
     3901 gactactaca aactcgccca ccccaacgaa ggcgtcggca gcggcttcga atacaaaaaa
     3961 gtcccccatg tcaccctgaa atccatcgcc aacaacgaac ctcccggcga agaaaccctc
     4021 tacgaccagc cgctcatcga caactctcgc gcccgcatct ccggcccctt caccgtcgaa
     4081 gccgtccccg cccccaacgt caaatccctc gacgacgttt ctgggtcttg tcatcccgaa
     4141 tccccgcgtt ctttgcgggg tgagggatct gctgtttccg gatctgcttc tccctccgcc
     4201 acgccgccgc aacccgccga cgcctccatc gcccgctccg gccctaccct tcgccaatcc
     4261 gactggcgtg acgaactcct caaaactggc ctccgcggca aaggcggcca gatgatcaat
     4321 ttctcccgcg tcgaacctct cggtggcacc cgctggctcc acgccgatgc cgaaacaaaa
     4381 ggcgtgaaac ccgagcgcgt cgtcatctcc ttcggcccgg attacgcacc actcgttacc
     4441 actcaggtcg aaatggcctg ggaagaagcc cgcactctcc accctaaacc cgaaatcctc
     4501 gtcttcgccg ccttccagtt cgatcccgaa gccgccaaag acatcgacga tctcaccaaa
     4561 gaaaaaaccg gcatgacctt cctaaccgcc caaatgaacg ccgacctcct caccgacgac
     4621 ctaaagaaaa aacgctccag cagccaatcc ttctggctag taggccgccc cgacgtagat
     4681 atccgccgcg ccaagtcttc cgattcctcc cgcttgtctt cctctgcctc ctctctgtca
     4741 tcccgaacga atgtgaggga tccgctttcc tccggcctct acatcgccga ggtccgcggc
     4801 ttcgactact acaacacccg caccggcacc atcgaatccg gcgacaccaa aaaaatcgcc
     4861 atgtggctcc tcgacaccga ctacgacggc cgcagcctct acccccgcca ggtcttcttc
     4921 cccctggccg acgaagacga aggctgggcc aaactagccc gaaacctcaa agccgaaatc
     4981 gatcccgacc tgatcgaaca ctatcgcggc actatctctt tgccgttcgc cctaggcgac
     5041 cacaaacgcg tagcagtaaa agtaatcgac gaccgcggca tcgaaagcct caaggtcgtg
     5101 caactatgac ctacactccc cattcttgtc attccgaacc cgccggctct ttatcacttg
     5161 tcactcaggc attttactgt cattccgaac ccgcggtctc attgcgggtg aggaatctgc
     5221 tttttccggt cttctcatga accacaacta cgccatctac atcctcgcca gccgttcccg
     5281 ctccctctac accggcgtga cagacaacct cgaacgccga atgatcgaac atcgccaagg
     5341 tctcgtcccc ggcttcacca ctcgttaccg agtcttccgc ctcgtccact tcgaatcctt
     5401 cggccacatt caagacgcca tcgcgcgcga aaaagaaatc aaaggttggc gccgcgaaaa
     5461 gaaaatcagc ctgatcgaac agcacaatcc cacgtgggag gacctagccg cacagctccc
     5521 acaccactat caatcacttc aacaccaaaa tcagatccct caacgctcgc caaacaccgg
     5581 cgaccatgtt cgggatgaca acctacacgt gtcattccgt gaca
//
LOCUS       DASYVO010000218         4586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_203893, whole genome shotgun sequence.
ACCESSION   DASYVO010000218 DASYVO010000000
VERSION     DASYVO010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203893"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VGR03_07190"
     CDS             complement(<1..605)
                     /locus_tag="VGR03_07190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV1994096.1"
                     /translation="MPDSSSLIGLTVSHYRIFEKLGGGGMGVVYKAEDTRLHRFVALK
                     FLPDDFAREPQALARFQREAQAASALNHPNICTIYDIGDHEVKAFIAMEFLDGVTLKH
                     LINSQPLETEKNLALAIEIADALDAAHAEGIVQRDIKPANIFVTKRGHAKILDFGLAK
                     VTLTNSSTSQAPGAIAGATLDDMHLTSPGTALGTVAYMSPE"
     gene            832..1374
                     /locus_tag="VGR03_07195"
     CDS             832..1374
                     /locus_tag="VGR03_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994097.1"
                     /translation="MKKTGIAFAVLAIILVSCDGAHAQRLAPKSSPVTIAPDLTKLNI
                     EKILQQVQTTKVQPSAQETSPLQVVAEFLQLRPGQVSELEQLLQTRQTTLVPIFQTAQ
                     ALIQQLAVLLNSGGNPAQVGILVIQIHALQQQMAQSQQAFFTQFVAMLDVEQLQKLQA
                     VQIAAQLQSILPAFQPIFLF"
     gene            1543..2082
                     /locus_tag="VGR03_07200"
     CDS             1543..2082
                     /locus_tag="VGR03_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV1994098.1"
                     /translation="MTGTCVTALIARADAHDPEAGFGKRIAENQRRVFQIAYGVLGNS
                     ADAEDVAQEAFLRAYQKFASLREAEKFRAWVNRIVFRLALNRQRGYRRRLARDTAWQI
                     NETQAMVDGAKDAEQRVMLERLRREIERLPQKFRSVLQLSLVEEMDAADVGAVLGIPA
                     GTVRSRVHTARKLLLEVMK"
     gene            2079..2582
                     /locus_tag="VGR03_07205"
     CDS             2079..2582
                     /locus_tag="VGR03_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994099.1"
                     /translation="MKNACEEWKDSLREAALTGTTAKDLEEHLRSCANCSAELDEVRA
                     RKVRLDALLPLVTQGAEPSANFRAQVLAAAAAASEGKRAQPWRVLTLAGATAVVVVVL
                     MIGVTSQRRTARMIPRDELAAAQKLAEWRAPSDGLLVTPGQEILRTMPRLGESYLNVP
                     VKTDEEE"
     gene            2584..3009
                     /locus_tag="VGR03_07210"
     CDS             2584..3009
                     /locus_tag="VGR03_07210"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEV1994100.1"
                     /translation="MRTGVLCICAIFLFAADTIRAQQPDQDPIGQSFFAPELVIQHQE
                     AIGLSAEQRDYFKAEIRQAQLKFTELQWKLQDEMEKLVGLVKQPRVDEQQVLAQLEKL
                     LAAEREVKKEQVTLLVRIKNKLTPEQQNKLSQLRPIVVK"
     gene            3385..>4586
                     /locus_tag="VGR03_07215"
     CDS             3385..>4586
                     /locus_tag="VGR03_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:3634..3636,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV1994101.1"
                     /translation="MGNARRERLTTPMIRANGKHRAASWDEALDRVAAGFRGEIEKHG
                     PRTFGMFSCSKTTNELNYVAQKFVRTVIGTNNIDSCNRTUHAPSVAGLAAVFGAGGGT
                     SSYREIEECEVVLLWGSNARDTHPIFFHHVLKGVRNGAKLYVVDPRRTSSAQWADVWL
                     GLDVGSDISLANAIGREIIASGLANEKFIKHATSGFDAYRECVENYTLERAEGETGVP
                     AEVIRQTAHAYAKAKSAQICWTLGITEHHNAVDNVVALINLALLTGHVGRYGSGVNPL
                     RGQNNVQGGGDMGAIPDRLPGFQHVENAELRAKFERAWGGMIPPKRGWHLSAMFDAME
                     RGELTTLYVLGENPVDSEADRHRAVKLLSGLKFMVVQDLFYTKTAQMADVILPGSAGW
                     CETEGTVT"
BASE COUNT         1034 a         1149 c         1514 g          889 t
ORIGIN      
        1 tgctcgggag acatgtaggc aacggtgccg agcgccgtgc ctgggctggt gaggtgcatg
       61 tcatctaaag tggccccagc gatcgcgccg ggagcctggc tcgtggaact gttggttagc
      121 gttaccttgg ccagcccaaa gtcgagaatc ttggcgtgac cacgcttggt gacgaagata
      181 tttgccggct taatgtcgcg ttggacgatg ccctcggcat gagcggcgtc gagggcatcg
      241 gcaatctcga tggcgagcgc caggtttttt tcggtttcca ggggctggct gttaatcagg
      301 tgcttgaggg tcacgccgtc aaggaattcc atggcgatga aggctttgac ttcgtggtca
      361 ccaatgtcgt aaatcgtgca aatgtttggg tggtttagtg cggaagcggc ctgggcttcg
      421 cgctgaaagc gagccagggc ttgaggttcc ctagcgaaat catcggggag gaatttcagc
      481 gcgacaaatc gatggagccg agtgtcttcg gccttgtaga cgacgcccat gccgccaccg
      541 ccgagcttct caaagatgcg gtagtgggac acggttagcc cgatgaggga ggaggagtcc
      601 ggcattaggc ggacatgtta ggttgtggac aaagacaagt caacgaagag ggccagtaaa
      661 tgcacggcac gggcgcacgg cattgatgaa ccttgatttg gccgctgaat tgctcccgtg
      721 ttggggagct tctatgtaga tcatgaacac acaattcgaa cgggaatggt ggccacggtg
      781 tcttgatgaa tgaaggcgca aaatgcgcgc acttcaagga gaaccgccgc catgaaaaag
      841 actggaatcg cctttgctgt gcttgccata attcttgtga gctgcgacgg agcgcacgcg
      901 caaaggcttg cacccaagtc ctcgccggtg acgatcgcac cggatctgac gaaattaaac
      961 atcgaaaaaa tattgcaaca ggtacaaacg acaaaagttc agccgagcgc ccaagaaaca
     1021 tcgcctttgc aggttgtggc tgaatttctc caactgcggc ccggccaggt gagcgagcta
     1081 gagcaattgt tgcagacgcg ccagacaacg cttgtgccga tctttcaaac ggcacaagcc
     1141 ctgatccaac agctagccgt tctgcttaat tccggcggga acccggcaca agtgggaata
     1201 ctcgtgatcc agattcacgc gttgcagcag cagatggcgc aatcacagca ggcttttttc
     1261 acacaattcg tggccatgct cgacgttgag cagttgcaga aacttcaggc cgtgcagatt
     1321 gctgctcaac tgcaatcgat tcttccagcg tttcagccga tctttctctt ctgatccacg
     1381 aggaccgcaa gaggccggat gtgggcggcg aggccgccgg catcaggctg caggaatctt
     1441 gcatgttgtg cgatttgccc gctacaccct gcgaccaggc gtgatatttt cctgaacgaa
     1501 cggcccggcc gcggtgtcta tacataaagg ggcaagagag gcgtgacagg cacgtgcgtg
     1561 actgcgttga ttgccagagc cgacgcgcat gatcccgagg cgggatttgg caagcgcatc
     1621 gctgagaatc agcggcgcgt ttttcagatt gcctatggcg ttctgggcaa ttccgccgac
     1681 gcggaagatg tcgcccagga agcgttcttg cgcgcctatc agaagtttgc ctcgctgcgc
     1741 gaagcggaga aatttcgcgc atgggtaaac cggatcgttt tccggttggc gctgaaccgc
     1801 cagaggggct accggcggag gctggcgcga gataccgcct ggcagatcaa tgaaacacag
     1861 gcgatggtcg acggcgcaaa agacgcggag caacgggtga tgctggaacg gctccggaga
     1921 gaaatagagc gcttgccgca aaaatttcgc agcgtcctgc agctttcgct ggttgaagag
     1981 atggatgccg ccgacgtggg cgcagtgctt ggcattccgg cggggaccgt gaggtcgcgg
     2041 gtgcacacgg cgcgaaaact tttgttggaa gtgatgaaat gaagaacgcg tgcgaagaat
     2101 ggaaagattc gttacgggag gccgcactga cgggtactac cgcaaaggat ctcgaagagc
     2161 atctgcgaag ctgcgcgaat tgctccgctg agctggacga ggttcgagcg cgcaaggtac
     2221 ggctggatgc gctgctgccg ctggtgacgc aaggagcgga gccctcggcg aatttccggg
     2281 cgcaggtgct tgctgcggct gcggccgcca gtgaagggaa gcgcgcgcag ccgtggcgag
     2341 tattgacgct ggccggagcg accgccgtgg tcgtggtggt actgatgatt ggggtgacct
     2401 cgcaacggag aaccgcgcga atgattccgc gggacgaact tgccgccgcg cagaaactgg
     2461 cggaatggcg agcgcccagt gatggcctgc tagtgactcc ggggcaggag atcttgcgga
     2521 cgatgccgag gctcggagaa tcctatctga atgttccggt gaagacggat gaggaggaat
     2581 gaaatgagga cgggagttct ctgcatttgc gcaatatttc ttttcgcggc ggatacaatc
     2641 cgcgcacagc agcccgatca agatccgatc gggcaaagct tctttgcacc ggaactcgtg
     2701 atccagcacc aggaagcgat tgggctgagc gccgagcaaa gagattattt caaggcggaa
     2761 atccgccagg cgcagttgaa attcacggag ttgcaatgga agctccagga tgaaatggaa
     2821 aaattggtcg gcctggtcaa gcaaccgcgc gtggacgagc agcaggtgct tgcgcagctt
     2881 gaaaaactcc tagcggcgga gcgggaagtc aagaaagaac aggtgacgct cctcgttcga
     2941 atcaagaata aactcacccc tgagcagcag aacaagctct cacagctccg tccgattgtc
     3001 gttaagtaag gattacagac aatccatttc tcaagaaaat tccgtctccc cctgcgcacc
     3061 gctctgctgg accctgcctt ttgccggatt tcaaactcag cgatcggtgt gggctatttg
     3121 agtttcttgt actcgtcccg ggcctgtttc agccatgccg ccaccgccaa gcttttcgac
     3181 gatgcggtag tgagagacgg tttgcccgat gggagaggag gagttcgaca ttaggcggac
     3241 atgttagatt gcggacggag acaaggcaac gaagagagtt ggtagaggca ggcgtcgatg
     3301 aaagagcata atatgtggct gtgaatcctg aattgacaga atgagcgcgc cgaagactca
     3361 gtagattggc gagagaggcg agatatgggc aacgcgagaa gagaacggct gacgacgccg
     3421 atgattcggg cgaacggaaa acatcgtgcg gcgagctggg acgaagcgct ggaccgggtt
     3481 gccgcgggat tccgcgggga gatcgaaaag cacggtccgc gcacgttcgg gatgttcagc
     3541 tgttcgaaaa ccacgaatga actgaattat gtggcgcaaa agttcgtgcg gaccgtgatc
     3601 ggcacgaaca acatcgatag ctgcaaccgc acttgacacg ccccgagcgt cgccggtctg
     3661 gcggcggttt tcggggcggg aggcgggacg agttcgtacc gcgaaatcga agagtgcgag
     3721 gtggtcctgc tgtggggctc gaacgcgcgg gacacacacc ccatcttttt tcaccacgtg
     3781 ctgaagggag ttcgcaacgg ggcgaagctg tacgtcgtgg atccccggcg cacgagttcg
     3841 gcgcaatggg cggatgtgtg gctgggcctt gacgtgggga gtgacatttc gctggccaac
     3901 gccattgggc gcgaaattat tgcgtcgggt cttgcgaatg agaagtttat caagcacgcc
     3961 accagtggtt tcgatgctta tcgcgagtgc gtggagaatt acactctgga gcgagccgaa
     4021 ggcgagaccg gtgttcccgc agaagtgatt cgacagacgg cgcatgctta cgcgaaggcc
     4081 aagagcgcgc aaatttgctg gacgctgggg attacggaac atcacaacgc ggtggacaac
     4141 gtggtggcgc tgatcaacct ggcgctgctg acaggacacg ttgggagata cggcagcggc
     4201 gtcaatccgc tgcgcgggca gaacaacgtg cagggcggcg gagacatggg cgcgattccg
     4261 gaccgcttgc ccggattcca gcacgtggag aatgcggaac tgcgcgcgaa attcgaacgt
     4321 gcgtggggcg ggatgattcc gccgaagcgc gggtggcatt tgagcgcgat gttcgatgcc
     4381 atggagcgcg gggaattgac cacgctgtat gtgttgggag aaaaccccgt ggattcggag
     4441 gcggaccggc atcgcgcggt gaagctgctg agcgggttga aattcatggt ggtgcaggat
     4501 cttttctaca cgaagacggc gcagatggcc gacgtaatcc tgcccggatc cgccggatgg
     4561 tgcgaaaccg aaggaacggt gacgag
//
LOCUS       DASYVO010000219         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_74957, whole genome shotgun sequence.
ACCESSION   DASYVO010000219 DASYVO010000000
VERSION     DASYVO010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74957"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1721
                     /locus_tag="VGR03_07220"
     CDS             <1..1721
                     /locus_tag="VGR03_07220"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1994102.1"
                     /translation="LKELRLLEPTMTHMSGTDHLSFNEAGVPGFWALQDGAEYSKTHH
                     SQSDTFDKVWKDDLNQGAQVLAAWAYNTAQLPGMLPRRPLPYNPSTDAAKAETPKPDP
                     IVEMDTKILEQVKADQANLKTNLQYLADRIGPRLTGSPQLDQASHWTMERFKEMGLSN
                     VHLEPWTIANSWTRGPATGRIVSPVETALTVAAAGWSPSSNGVVRGAVVGLTYEKLED
                     LEKYRGKLKGVIILLGHPREMEAPGNPMTTPWGEETIPIAHPKGEAPYVSGDYRKVRM
                     AITKMISDEKPLAVLIGSEKEYELLNMTTYSRDYQPAGAPVAYVARENYRLLWRFLDE
                     GPVQVEVDIQAKLGGKTVEVYNTVAEIPGSEKPDEVVIIGGHLDSWDLGTGATDNGTG
                     SMAVLEAARALQKLGVKPKRTIRFVLFTGEEQGLNGSKAYVKAHVAELGKISGVLVHD
                     SGTGKVLTVGLMGNYGARETIDHALYPLGRAKEVGLSEPTLRSEGGSDHVPFDEAGVP
                     GFWCVQDPVDYDKTHHSQADTIERVRWDDLTEGAQVLAVFAYNVAQLPEMLPRKPAKA
                     VQAGSN"
     gene            1731..2180
                     /gene="dtd"
                     /locus_tag="VGR03_07225"
     CDS             1731..2180
                     /gene="dtd"
                     /locus_tag="VGR03_07225"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEV1994103.1"
                     /translation="MRAVLQRVSRAKVTVEERVTGEVGAGLMILLGVGREDTSAVAAA
                     MAEKCANLRIFEDEQGKMNRSLLEVKGSALVVSQFTLYGDARGQRRPSFLSAAPPELA
                     KALYEEVCEALRKLGVNVATGIFQTMMSVELVNEGPVTILLDSDKTF"
     gene            2248..>2603
                     /locus_tag="VGR03_07230"
     CDS             2248..>2603
                     /locus_tag="VGR03_07230"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV1994104.1"
                     /translation="MADPKVRAKMAYFGVNVPKAYGISAPVLHRFARQIGKDHQLARE
                     LWLTGTHEARILATLIGDPEKVNAAEMERWVRDFDSWDLVDAACCYLYAHSKLAWKMA
                     DAWSFRREEFVKRAAFS"
BASE COUNT          604 a          669 c          851 g          479 t
ORIGIN      
        1 cgttgaagga gctaaggctg ctcgagccga cgatgacgca tatgtcgggg acggaccacc
       61 tgtctttcaa cgaggcgggg gttcccggtt tctgggcgct tcaggacggc gcggaatact
      121 cgaagacgca ccacagccag tctgacacct tcgacaaagt gtggaaggac gatctgaatc
      181 aaggcgcgca ggttttggcg gcgtgggcct acaacacggc gcagctgccc ggcatgctgc
      241 cgcggcggcc gctgccctac aatccttcga cggatgccgc aaaggcggaa acgcccaagc
      301 ccgatccgat tgtggagatg gatacgaaga ttctggagca ggtgaaggcg gatcaagcga
      361 atctgaaaac aaatttgcag tatttggcgg accgcatcgg cccgcggttg acgggatcac
      421 cgcaactcga ccaagcaagt cactggacga tggaacgatt caaggaaatg ggtctttcga
      481 acgtgcacct ggagccatgg acgattgcga acagttggac gcgaggacca gcaacgggcc
      541 gaatcgtctc gccggtcgaa accgcgctta cggtggctgc ggcaggatgg tctccaagct
      601 caaatggtgt ggttcgcggt gcggtagtgg gactgacata cgaaaagttg gaagacctgg
      661 aaaaataccg gggaaaactg aagggtgtga ttatcttgtt aggacacccg agagaaatgg
      721 aggctccggg aaatccaatg accacgcctt ggggagaaga gacgatccct attgcccatc
      781 ctaagggaga agcgccgtac gtctctggcg actatcggaa agttcggatg gcaattacga
      841 aaatgatttc cgatgaaaaa cctttggccg tactcattgg ctccgagaag gaatacgagc
      901 tgcttaatat gaccacgtat tcccgggact atcagccggc gggcgctccg gtcgcctatg
      961 tcgcgcggga gaactaccgg cttttgtgga gatttctgga tgaaggtccg gtgcaagtgg
     1021 aagtagacat tcaggcgaaa ctcggcggga agacggtgga ggtgtacaac acggttgcgg
     1081 aaattccggg aagcgagaag ccggacgaag tggtgattat cggagggcat ctcgattcgt
     1141 gggatttggg aaccggcgcg accgacaacg gcaccggctc catggcggtg ctggaagccg
     1201 cccgcgcgct gcagaaactg ggcgtgaagc ccaagcgaac gatccgattt gtgcttttca
     1261 ccggcgaaga gcaggggttg aatggctcaa aggcttacgt aaaagctcac gtcgccgaac
     1321 ttggaaaaat ttccggcgtg ctggtgcacg acagcgggac gggcaaagtg ctgaccgttg
     1381 gattgatggg aaactacgga gctcgggaga ccatcgacca cgcactctat ccgctgggcc
     1441 gggcgaagga agtcggattg tcagagccga cgctgcgttc cgaaggtgga agcgatcatg
     1501 tgccgtttga tgaagccggc gtgccgggat tctggtgcgt gcaggatccc gtggattacg
     1561 acaagacgca ccactcccag gcggacacga tcgaacgcgt gcgctgggac gacctgacgg
     1621 aaggggcgca agtgctggcg gtttttgcct acaacgtcgc gcaactgccg gagatgttgc
     1681 ctcggaagcc cgcaaaggcg gtccaagcgg gcagcaatta agctggttgc atgcgagcgg
     1741 ttctccagag agtcagccgc gcgaaagtga cggtcgaaga acgcgtgaca ggcgaagttg
     1801 gcgccgggct gatgattctg ctcggcgtcg gccgcgaaga tacgtcagcc gtcgccgcag
     1861 cgatggcgga aaaatgcgcg aacctgcgca tcttcgaaga cgagcagggt aagatgaacc
     1921 gttcgctgct ggaggtgaaa ggcagcgcgc tggtcgtctc gcagttcacg ctttacggcg
     1981 atgcgcgcgg gcagcggcgg ccaagcttcc tctcggccgc gccgccggaa ctagccaaag
     2041 ctctctacga agaagtttgc gaagcgcttc gcaaacttgg cgtgaacgtc gcaaccggta
     2101 ttttccagac gatgatgtcc gtggagctgg tcaatgaagg cccggtcacg atcctcctcg
     2161 attccgacaa aacgttctag gcagagccac tctcgaagga tagcggctca gtggaccaaa
     2221 gcggccgtgc tccgcgaatt gaaggcattg gcagacccaa aggtgcgcgc gaagatggcc
     2281 tacttcggcg taaatgttcc gaaagcgtac gggatctccg cacctgtctt gcatcgattc
     2341 gcgagacaaa tcgggaaaga ccatcagctg gcccgggaac tttggttaac aggcactcac
     2401 gaagcgcgaa tcctggcaac gttgattgga gatccggaaa aggtcaacgc agcggagatg
     2461 gaacgctggg tgcgggattt cgattcctgg gacttggtgg atgcggcttg ctgctatctt
     2521 tatgcgcact ccaagctggc ctggaaaatg gctgatgcct ggagctttcg ccgggaagaa
     2581 ttcgtaaagc gagccgcatt ctc
//
LOCUS       DASYVO010000220         4365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_175300, whole genome shotgun sequence.
ACCESSION   DASYVO010000220 DASYVO010000000
VERSION     DASYVO010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175300"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="VGR03_07235"
     CDS             complement(<1..1340)
                     /locus_tag="VGR03_07235"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEV1994105.1"
                     /translation="MHFFGQHILSVVLFTPLVGALLLLFIPREMERVHKIAGNVFGVL
                     GFLVSLPLLRWFKPEWGGFTFEETAEWIPSIGAKYHLGIDGISLLLIMLTTLLGMIAI
                     LSSWSAIHLRTKEYYILLLLLQTGMLGVFVSLDFFLFYVFWEVMLVPMYFLIGVWGSD
                     RRLYAAIKFFLYTLAGSVVMLLAILALYFYAPVAAGATRSFDVPTLLAAAQHFTDPLK
                     VWLFWGFFFAFAIKVPMFPFHTWLPDAHTEAPTAGSVILAGVLLKMGTYGFIRFSLPL
                     LPTNPAMRASIIHVVIVLSLIGIIYGALVCLMQKDMKRLIAYSSVSHLGFCTLGIFAL
                     TPNGLAGSVLQQINHGISTGALFLIVGVLYERRHTRMISEFGGLATPMPNFAAIYLIV
                     SLSSLGMPLLNGFIGEFTILQGAFQVSKAWAAWGSLGVVLGAAYLLWLYQRVMFG"
     gene            complement(1353..3476)
                     /gene="nuoL"
                     /locus_tag="VGR03_07240"
     CDS             complement(1353..3476)
                     /gene="nuoL"
                     /locus_tag="VGR03_07240"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEV1994106.1"
                     /translation="MTPTGYFLDHLWMIPLFPLATAALMLFVGNRLPKTAVSLFCVGS
                     VFVSFLFAVGAVLQLLAADPEHRVAQQIMFEWLTPGPMQMTGGHAIQFAADWGFLLDP
                     LSCVMVLVVTGVGFLIHVYSVGYMAHEGGYYRFFGYMNLFMFAMLTLVLANNMLLMFV
                     GWEGVGLCSYLLIGFYFLKESASDAGKKAFIVNRIGDAGFILGILLVALTLGTIRFTA
                     QGLPGGAGYSGIRQVLETARDSGTLGIIAPTMTAIALLLFVGAMGKSAQIPLYVWLPD
                     AMEGPTPVSALIHAATMVTAGVYMVARMNAVYRLSPFAMDVVAVVGVVTAIFAASMAL
                     VQNDIKKVLAYSTISQLGYMFLALGVGAFSAGIFHLMTHAFFKALLFLGAGSVIHAMS
                     GEQDIQKMGGLWDKIPITARTFCAATLAIAGIPPLAGFFSKDEILGQAFEKHPALWVV
                     GFITAGMTAFYMFRLLFLTFFGYSRADEHVEKHIHESPYTMTVPLMILAGLSVIGGWI
                     GWPASLFGSNHFEHFLEPVLGSAGAELPVESAVPHASLTEIFLMVLSVAVVGAGIWLA
                     YQFYRTKRIAPELVAGKWPKVYQLLVHKYYVDEIYDAAVVERTKDLGTLLGRFDANVI
                     DGLGVDGAGWLARFGSTLSMWWDKWIIDGLLNFSAKLMQLFSYPVRLLQTGMFSSYAM
                     LILAGLVVLLAYYGHHVQVLLRSVR"
     gene            complement(3509..3811)
                     /gene="nuoK"
                     /locus_tag="VGR03_07245"
     CDS             complement(3509..3811)
                     /gene="nuoK"
                     /locus_tag="VGR03_07245"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEV1994107.1"
                     /translation="MLPIGHYLFLSLALFIIGVIGVVTRRNVIVVLMSIELILNAVNI
                     NLVAFSRLYGDVAGQVFALFIIADAAAEAAVGLGIIIAFFRNRETVLADEMDLLKW"
     gene            complement(3819..>4365)
                     /locus_tag="VGR03_07250"
     CDS             complement(3819..>4365)
                     /locus_tag="VGR03_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEV1994108.1"
                     /translation="RIQTPLRIFMLDKVLFYFFAGIAVASAVLMVTRRNVIHSAIFLI
                     TALLATAGIFLQLRAEFLFIVQIILYVGGIMVLFVFVIMLVNLDVALQQIQFARQKWV
                     ALLVALALAGQVGLMFWSSGKMPGQGLPVLTAAAAARLPLNSEAVAHSLFESYLLPFE
                     IASVLLLVAMIGAVVMAKKRT"
BASE COUNT         1133 a         1309 c         1194 g          729 t
ORIGIN      
        1 ccgaacatca cgcgctggta gagccacagc aggtatgccg cgcccaacac aacgcccagc
       61 gagccccaag cggcccaggc cttgctcacc tgaaatgcgc cttgcagaat cgtgaactct
      121 ccgatgaagc cgttgagcag cggcatcccc agcgaactga ggctgacgat cagatagatg
      181 gccgcgaaat tgggcatggg cgtcgccaag ccgccaaact ccgaaatcat gcgcgtgtga
      241 cggcgctcgt agagcacgcc cacaatcaag aagagcgcgc cggtggagat gccgtggttg
      301 atctgctgga gcacgctgcc cgccaggccg ttcggcgtga gcgcaaaaat tcccagcgtg
      361 cagaagccca gatggctgac cgagctgtag gcaatcaggc gcttcatgtc cttctgcatg
      421 aggcagacaa gcgcgccgta aatgatgccg atcaaagaga gcacgatgac gacgtgaatg
      481 attgatgcgc gcatcgcggg gttcgtgggc agcagcggaa gcgagaagcg gatgaaaccg
      541 tacgtgccca ttttcaagag aacgccggcg aggatgacgg agccggccgt gggcgcttcg
      601 gtgtgcgcgt cggggagcca tgtgtggaac gggaacatgg gaaccttgat ggcaaacgca
      661 aagaagaaac cccagaagag ccacaccttc agcgggtcgg tgaagtgctg agcggcagcg
      721 aggagcgtcg gaacatcgaa gctacgtgta gcgcctgccg ccacgggcgc ataaaagtag
      781 agcgccagga tcgccagcag catgaccacc gatccggcca aggtatacag gaaaaactta
      841 atggccgcgt agaggcgccg gtcgcttccc caaacgccta tcaggaagta catcggaacg
      901 agcatcactt cccagaacac ataaaagagg aagaaatcca gggaaacaaa aacgccgagc
      961 atcccggtct gcagcagcag gagcagaatg tagtattcct tggtacgaag gtgaatcgcg
     1021 ctccaggacg agaggatggc gatcatcccg agaagcgtgg tgagcatgat gagcaagagg
     1081 ctgatgccgt cgatgccgag atggtatttc gcgccgatcg atgggatcca ctcggcggtt
     1141 tcctcaaagg tgaagccgcc ccattccggt ttgaaccagc ggagcagcgg aagcgagacc
     1201 aggaaaccga gcacgccaaa aacgtttcct gcgatcttgt gcacgcgctc catttcgcga
     1261 ggaataaaca gcagcagcaa ggcgcccacg agcggcgtaa acaaaaccac gctcaagata
     1321 tgttgtccaa aaaagtgcat gctctttgcc tctcagcgca cgctgcgcag caatacctgc
     1381 acatggtgcc cgtaatacgc cagaagaacg actagcccgg ccaaaatcag catcgcgtag
     1441 ctggaaaaca ttccggtttg cagcaagcgc accggataac tgaaaagctg catcaacttc
     1501 gcgctgaaat tcagcagccc atcaatgatc cacttgtccc accacatgga tagtgttgag
     1561 ccgaaacgcg ccagccagcc cgcgccgtcc acgcccagcc cgtcaattac gttggcgtcg
     1621 aagcggccga gcagagttcc gaggtctttc gtgcgctcga cgactgccgc gtcatagatt
     1681 tcgtccacgt aatacttatg cacgagcaat tgatagactt tcggccattt accggcgacc
     1741 aattccggcg cgattctctt ggtccggtag aactggtacg ccagccagat tccggcgccg
     1801 acgacggcaa ccgacagaac catcaggaaa atctccgtca acgaagcatg aggcaccgcg
     1861 ctttccacag gcaactccgc tcccgcactc cccagtaccg gctccaggaa gtgttcgaag
     1921 tgattcgaac caaaaagtga cgccggccac ccaatccatc cgccgatcac cgagagacca
     1981 gcgagaatca tcagcggcac ggtcatcgtg tacggcgatt cgtggatgtg tttctccaca
     2041 tgttcatccg cgcgcgagta accgaaaaat gtcaggaata gcaaccggaa catgtagaac
     2101 gcggtcatcc ccgcggtaat gaagccgacg acccacagag cggggtgctt ctcaaaggct
     2161 tgacccagaa tctcgtcttt gctgaaaaaa ccggccagcg ggggaattcc tgcaatcgcc
     2221 aaggttgccg cgcagaatgt tctcgcggtg atgggaattt tgtcccacaa tccacccatc
     2281 ttttgaatgt cctgttcgcc ggacatggca tgaatcacgc tgccggcacc gaggaacagc
     2341 agcgctttaa aaaacgcgtg ggtcatcaga tgaaaaattc ctgcgctaaa cgcgcccacg
     2401 cccaaggcca gaaacatgta cccaagctgg ctaatcgtcg aataggcaag cactttcttg
     2461 atgtcgtttt gcaccagcgc catggatgcg gcaaagatcg ctgtcactac gccgacgacg
     2521 gccaccacgt ccatcgcaaa aggtgagagc cggtagacgg cattcatgcg cgccaccatg
     2581 taaacgcccg cagtcaccat tgtagcggcg tgaatcagcg cactcaccgg cgtaggccct
     2641 tccatcgcgt ccggcagcca aacatacaag ggaatctgcg ccgatttccc catcgctccc
     2701 acaaatagca agagcgcgat ggccgtcatc gtcggtgcga tgattcccag cgtcccggaa
     2761 tccctggccg tttctaacac ctgccggata cccgaatagc ctgcgccacc gggcaacccc
     2821 tgagcggtga agcgaatcgt tccgagcgtc agtgcaacca gcaaaatccc gaggataaag
     2881 cccgcgtcac cgatgcgatt cacgatgaac gcttttttcc ccgcatcgga ggccgactct
     2941 ttcaagaaat agaaaccgat cagcaagtag ctgcacagac cgacgccttc ccagccgacg
     3001 aacatgagca acatgttgtt cgccaggacc agcgtcagca tggcgaacat gaagagattc
     3061 atgtagccga aaaagcggta gtacccgcct tcgtgcgcca tgtacccgac ggagtagacg
     3121 tgaatcagga agccgacacc ggtgacgacc agaaccatga cgcaggaaag cgggtccaga
     3181 aggaatcccc agtccgccgc aaactgtatg gcgtgtcccc cggtcatctg catcggcccc
     3241 ggcgtcagcc actcgaacat gatttgctga gcgacgcgat gctccggatc ggcggccaac
     3301 aattggagga ctgctcccac cgcgaaaaga aacgacacaa aaacgctgcc aacgcaaaac
     3361 aaactgacag cggtttttgg cagccggttg cccacaaaaa gcatcagcgc cgccgttgcc
     3421 agcggaaaca gcgggatcat ccaaagatga tcgagaaaat agcccgtggg cgtcatgccg
     3481 ttttcatttc ttctttcatg tcgtcacgct accacttcaa caaatccatt tcgtccgcca
     3541 acaccgtttc gcggttgcgg aaaaaagcga tgatgatccc cagccccacg gcggcctctg
     3601 cggccgcatc ggcgatgatg aagagcgcga agacctgccc cgccacatca ccgtacaaac
     3661 gtgaaaatgc caccaggtta atgttcacgg cattcagaat cagctcgatg gacatgagca
     3721 cgacgattac gttgcgccgc gtgacaactc cgatcacgcc aatgatgaag agcgcgagac
     3781 tcaggaacag gtaatgtcca atcggaagca tcgctgcgtt acgtcctttt cttggccatt
     3841 acgacggccc caatcatggc cacaagcaag agaaccgacg caatttcaaa tggcaataga
     3901 tacgattcga acaggctgtg tgccaccgct tcggaattga gcggcaaacg cgccgctgcc
     3961 gccgccgtca gcaccggcaa cccctgcccg ggcatcttcc cggaagacca gaacatgagg
     4021 ccgacctggc cagccagggc cagcgcgacg aggagcgcca cccacttctg ccgcgcgaac
     4081 tggatctgct ggagcgccac atccaaattg accagcatga tcacgaagac gaacagcacc
     4141 atgatgccgc caacgtagag aatgatctgg acgataaaca aaaactccgc gcgcaactgc
     4201 aagaaaatcc ccgcggttgc gagcagcgcg gtgatcagaa agatggcgct gtgaatgacg
     4261 tttcgccgcg tcaccatgag caccgcgctg gccacggcga tgccggcaaa gaaataaaat
     4321 aggaccttat ccagcataaa aatccgaagc ggcgtttgga tcctg
//
LOCUS       DASYVO010000221         3616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_19661, whole genome shotgun sequence.
ACCESSION   DASYVO010000221 DASYVO010000000
VERSION     DASYVO010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19661"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            524..1930
                     /locus_tag="VGR03_07255"
     CDS             524..1930
                     /locus_tag="VGR03_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV1994109.1"
                     /translation="MRYPLPNKSTVWIQTRPANISANASKPNATRAAVRKAALLPFVF
                     VMYAYATGGPFGLEDMVTTSGPGLTLLYQLFIPLFWCIPVSLVAAELTTAIPVEGGSY
                     RWVRSAFGDFWGFQAGWWNWSASFLLGGTYGVLTADYLTFFFPGLVGWKHYAIAVAVI
                     ALIGYINVRGIQMVGRVATILEISILLPIVAMCVIAATKWHHNPFSPLVPPHVPPFQV
                     FGVGLALGLWLYSGYEQVSSVAEEVENPQRSYPIALAVVIPLSIATYFLPTMFSLAAL
                     GDWQKWHTGYFSEAAQLVGGPWLGFAMIIAALITNLSLLNATVLTSTRMPSTMAEDGY
                     LPEAFSARHPRYGTPWIAIIVSSIIYALLAQKTMVQLLTVYVWLRIGVTILTVLSSWQ
                     LRKTQPDLPRPFRIPWGRAGLLYVIVAPLVMSVVALVASDPFARRWGPVTVVLGPVMY
                     FVLRKLQAGKIGAGQQPT"
     gene            complement(1923..3221)
                     /locus_tag="VGR03_07260"
     CDS             complement(1923..3221)
                     /locus_tag="VGR03_07260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV1994110.1"
                     /translation="MPNQEKNFWATTVDSPSFAASPELPDSADVAVVGAGFCGLSAAR
                     ALAKRGVKVSVLEAETFGWGASSRNGGMVLTGMKLPVPTLIKRYGRETVRKMYAASLD
                     SIDCVEQIVREENIDCNFSRCGHLEVACKQAHFDGYEESAALIQREFNHELRIIPKSE
                     LRGEIGSDIYFGGMVDETSAGLNPARYVAGLAHAAQRAGACLYDRTRVTSVELERNNN
                     ARKFLVGTSKGAITAREVILASGAYTTEATPALRKKIIPIGSYIIATEVLPADLAHEL
                     SPRNRMIYDSKHFLYYYRLTPDNRMLFGGRAAFFPETENTVRQSAEILRRGMIDVYPQ
                     LREAKVEFVWGGTLDFTLDVMPHAGKIDGMYFAAGFAGHGVAAATWFGTKLAGLICGD
                     PNDIPFDGIKFSAAPFGLRSGNTWALPLAGAWYRILDFLT"
     gene            complement(3373..>3616)
                     /locus_tag="VGR03_07265"
     CDS             complement(3373..>3616)
                     /locus_tag="VGR03_07265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEV1994111.1"
                     /translation="GNFGSGWIAAFNGFTHKFIGWVRNPDDSLVTIDGLWSLTFGNDA
                     TAGPANTLFFTAGINNEQDGLFGTLTPLDGLDGDEE"
BASE COUNT          673 a         1058 c         1060 g          825 t
ORIGIN      
        1 ctaggccagc tcttatccgt tcggacaatc cgtgtggctt ccgcgtactg tcttgttttt
       61 tgcgagggta gggcatagtt cgtataccat ccgccagcgc cgttcaacgc tccacgcgac
      121 cgttcaatgc gaaatttcta ccattcgatg ctaattactt aacctttcca tactggcatg
      181 ctattctttc ccgtgttggc cagttcacat ttgcggattt cttcccgccc ttcgcccgct
      241 ttactcggct gtccgtctaa cttccatgga atcatatcgt ttgcagaccc ccaccctcta
      301 acccttttgg aatcatatcg tttcaaaaac atagggggga gggggcacct ccagcttgca
      361 acgccagcaa cgcccaaaat cccgacgcgc atccatttgt cttttcaatc acttgcgaaa
      421 tgctctctgc gcaactcctt ttttttgata accttccatt ttcatggggg ggtgtacccc
      481 cctttggcac tcccgcagca ctccctaccc accgacaggc acaatgcgct accctctgcc
      541 gaacaaatcc acggtctgga ttcaaacgag gccagcaaac atttccgcca acgccagcaa
      601 acccaacgca acgcgcgcgg ccgtgcgcaa ggctgcgttg cttccgttcg tcttcgtgat
      661 gtacgcctat gcgactggcg ggccgtttgg gctcgaggac atggtgacca ccagcgggcc
      721 ggggctgacg ctgctctatc agctgttcat tcccctgttc tggtgcattc ctgtttcgct
      781 cgtcgccgcg gagctgacca ccgcgattcc cgtcgagggc ggatcgtacc gctgggtgcg
      841 ctcggccttc ggcgacttct ggggattcca ggccggctgg tggaactgga gcgcttcgtt
      901 cctgctgggc gggacgtacg gcgttctgac cgcagactat ctcacgttct ttttccccgg
      961 actggtcggg tggaagcatt acgcgattgc cgtcgccgtg attgcactga tcggctacat
     1021 caacgtgcgc ggcattcaga tggttggcag ggttgcaacg attctggaga tttccatcct
     1081 gctgccgatc gtggcgatgt gcgtgatcgc ggcaacgaag tggcaccaca atccgttttc
     1141 gccgttggtt ccgccgcacg ttccgccgtt tcaagtcttc ggcgtggggc tggcgctggg
     1201 cttgtggctc tactccggct acgagcaagt gtccagcgtt gcggaagaag tggaaaaccc
     1261 gcagcgcagt tatccgatcg cgctcgcggt ggtcattcct ctttccatcg ccacatattt
     1321 tctgcccacg atgttttcac tcgcggcgct cggcgattgg cagaagtggc acaccggata
     1381 tttttcagag gccgcgcaac tcgtcggcgg gccgtggctg ggttttgcca tgatcatcgc
     1441 ggcgctgatc accaatcttt cgctgttgaa cgccacggtg ctgaccagca cgcgcatgcc
     1501 atccacaatg gcggaggacg gctatcttcc cgaggcgttt tcggcgcgtc atccgcgcta
     1561 tggcacgccg tggatcgcca tcatcgtgtc ttcgatcatc tacgcgctgc tggcacagaa
     1621 aacaatggtg cagcttctga ccgtctacgt ctggctgcgc atcggagtaa cgattctgac
     1681 cgtgctttcc tcgtggcagt tgcgcaagac gcagccggat ttgccgcgcc ccttccgcat
     1741 cccctggggc cgcgccggac tgctttacgt gatcgttgca ccactggtga tgagcgtggt
     1801 cgcgctggtg gccagcgacc cgtttgcgcg acgatgggga cccgtaacgg tggtgctggg
     1861 accggtaatg tattttgttc ttcgcaaatt acaggcagga aaaatcggcg ccggccaaca
     1921 gcctacgtaa ggaaatcgag gattctgtac cacgcgcccg ccagaggaag cgcccaggtg
     1981 ttgccgctgc gcaagccgaa tggagcggcg gaaaacttga tgccgtcgaa gggaatgtcg
     2041 ttgggatcgc cgcagattag cccggcgagt ttcgtaccga accaagtcgc cgcggcaacg
     2101 ccgtggcccg caaagcccgc agcgaaatac atgccgtcga tttttcccgc atgcggcatc
     2161 acatcgagtg tgaaatcgag cgtcccaccc cagacgaatt caaccttggc ttcgcgcagt
     2221 tgcgggtaca catcgatcat gccgcggcgg agaatttccg cgctctggcg cacggtgttt
     2281 tcggtttccg gaaagaaggc cgcgcggccg ccgaaaagca tgcgattgtc cggcgtgagg
     2341 cggtagtagt agagaaagtg tttcgagtcg tagatcatgc ggttgcgcgg gctgagttcg
     2401 tgggcgagat cggcaggcaa aacttccgtg gcgataatgt acgagccgat aggaatgatt
     2461 ttttttcgca gcgcgggagt ggcttctgtc gtgtacgcgc cgctcgcgag aatcacttcg
     2521 cgcgcggtga tcgcgccttt tgaagtaccc accagaaatt tgcgcgcatt gttgtttctc
     2581 tctagctcga ccgatgtaac gcgcgtgcga tcgtaaaggc acgcgcccgc ccgctgcgcg
     2641 gcatgcgcga ggccggcaac gtaccgcgcg ggattcagcc ccgcgctggt ttcgtccacc
     2701 atgccgccga aataaatgtc cgagccgatt tcgccgcgca gctcgctttt tggaatgatg
     2761 cgtaattcat gattgaattc gcgctggatg agggcggcgg attcttcgta gccatcgaag
     2821 tgcgcttgct tgcacgccac ttccaggtgg ccgcagcgcg agaaattgca gtcgatgttc
     2881 tcttcgcgaa cgatctgctc gacgcaatcg atggagtcga gagaggcggc gtacatcttg
     2941 cgcacagttt ctctgccgta gcgcttgatc agcgtgggca ccggcagctt catgccggtg
     3001 aggaccatgc caccgttccg cgagctagcg ccccagccaa atgtttcggc ttccagcacg
     3061 gaaactttca cgccgcgttt cgcgagcgcg cgcgcagcag aaagtccgca gaatcccgcg
     3121 cccacgacag caacgtcggc agaatccggg agttcaggtg aggcggcgaa agaaggcgaa
     3181 tcgactgtgg tcgcccagaa atttttctct tggttaggca tcggctgaat tggcgtgagg
     3241 agattctagc attgacggca ggttgaaaaa caatcggcgg gatcagcgca gaaaaaaaga
     3301 gacggcagtg gatattgtcg atcctgccgt ctcgttgcgt gctgtttatt tgtagtaggg
     3361 aacttgccgt atttactctt cgtcgccgtc gagaccgtcg agaggagtaa gggtgccgaa
     3421 taggccgtcc tgttcgttgt tgatccccgc ggtgaagaac aaggtgttgg ccggtccggc
     3481 agtcgcgtcg ttgccgaagg tgagcgacca caagccgtcg atggtcacga gcgagtcgtc
     3541 ggggttcctc acccagccga taaatttgtg ggtgaagccg ttgaatgcgg cgatccaacc
     3601 actgccgaag ttgccc
//
LOCUS       DASYVO010000222        20338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_19678, whole genome shotgun sequence.
ACCESSION   DASYVO010000222 DASYVO010000000
VERSION     DASYVO010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 20338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20338
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19678"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..709)
                     /locus_tag="VGR03_07270"
     CDS             complement(29..709)
                     /locus_tag="VGR03_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV1994112.1"
                     /translation="MAISRRALIKTGAGAACAMVATGAHAIQEAKQPEIAKATSADRL
                     PPIPPDKMTEAQKKAAADFAAGRGTPMFGPFIPLLRSPEVMLAAKALGDHLRYKSALP
                     ENLNEFAMLITARRWSQQYEWAVHYPIALKAGVSPEIAKAVAEGRRPQGMSPDEENIY
                     EFCTELHQNQGVSDATYARVLAKYGEPGVIDLVGVAGYYTFLAMVLNVARTPVTQGSA
                     PPLPAFPR"
     gene            complement(873..1406)
                     /locus_tag="VGR03_07275"
     CDS             complement(873..1406)
                     /locus_tag="VGR03_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994113.1"
                     /translation="MKGYVQWFATVALAVIIIAGAERVPALKRADSFTEQHRRPLVAA
                     TLAVTILGFLVFMGGGVYWSLKAGAQPGDGEAGQTSGQLQYQRPFFSWYRFWGKTWSW
                     GFEDEASIAEVKQARKGWWHNPRWRRNFVLMGSGLTMGLGLFALMAVLATVGVKLLIA
                     AALLYALVRLTWAFSHA"
     gene            1846..3555
                     /gene="rpoD"
                     /locus_tag="VGR03_07280"
     CDS             1846..3555
                     /gene="rpoD"
                     /locus_tag="VGR03_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV1994114.1"
                     /translation="MTEEKADQVRNLINLGRERGYLLYDEVKEVPSGEAQSPEEIDSL
                     VSALERYGIDIYEDVSAAKAAHAVHDAAESTEVEARKEVAESEEVELNLTPGIVEKNS
                     NPVGMYLREMGTVPLLTREGEVAIAQRIERGHLLVLKTISRSPIVLKALLAIGEELRK
                     GTRSIKEFVHFSAEELTERDIESKTRHTLRIIAKIEKLYGVGLKQAARLEKLPKSRKR
                     AYLRARRQLARTRVAMSLQVRAIDFHPLQKKRLINKMRHTVEHLHWLEREAARLERRA
                     TASRGDAAAEARKELRARRSELREIEEASEVGLTVLKRSLALVQRGEAEAEQGKKELT
                     EANLRLVVSIAKKYTNRGLEFLDLIQEGNIGLMTGADKFEWRRGYKFSTYATWWIRQA
                     VTRAIADKARTIRVPVHMIEAINKQVRTSRQLVQELGREPTAEEIAKRMDIAVDKVRK
                     TKKIAQQPLSIETPIGEEGDSHLGDFIEDKAVVSPADAVITLNLKEQTASLLKTLTPR
                     EERIIKMRFGLEDGSEHTLEEVGQSFAVTRERIRQIEAKALCKLRHPSRSSRFRVFLE
                     GPS"
     gene            3635..3988
                     /locus_tag="VGR03_07285"
     CDS             3635..3988
                     /locus_tag="VGR03_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV1994115.1"
                     /translation="MKNLFVGNMSFQTTESDLQEMFKAFGQVTRVHVAIDRETGRARG
                     FAFVEMPNDEEAAKAVAALNGKEVGGRSLKVNEARPKTERSAPRSSGGGGRNRGGFSN
                     EDYRESARQPREPRW"
     gene            4054..4275
                     /locus_tag="VGR03_07290"
     CDS             4054..4275
                     /locus_tag="VGR03_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994116.1"
                     /translation="MTVTFQKRQKEMKRLEKQRAKAERRAQKKLEKRARSEAPNETGL
                     GETALGETELGETALGAAAAEPSTAEPKI"
     gene            complement(4433..4945)
                     /locus_tag="VGR03_07295"
     CDS             complement(4433..4945)
                     /locus_tag="VGR03_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994117.1"
                     /translation="MKATRWILLVACSALLLGCGKSGKLAPLLPDAPAGWTAEGTANR
                     DVSGVGHSSTRSYVPTGATAGMGAKRVTVQVLLAEKGADQKKLQQMSIENKAQFKERK
                     EVGGFPAYESFPLPGNETHSLDILPRAGTYVQIVAYQGGPAWDKGENRQAVVSAFAGK
                     IDLKKIATLE"
     gene            complement(5213..7030)
                     /gene="polX"
                     /locus_tag="VGR03_07300"
     CDS             complement(5213..7030)
                     /gene="polX"
                     /locus_tag="VGR03_07300"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEV1994118.1"
                     /translation="MDNKQVARILRETAQLLEIDGAIIGRYRSYEKAAELIDSLPESI
                     AALAQQPEKLEALPGIGERMAAHLQEIVAKGDYGLRRKLLKKYPATLLDLLTLQSLGP
                     KKVALLWKTFRAGTVAEVEKLAREGKLRDLAGFGEKSEQNILKAVEVFKKSSGRFLLN
                     VAEAAAQQLVAHIEKAGKTVESIMPAGSLRRGKETVGDLDLLVTLAPGHTSQKHVDSL
                     AAHILKFPGIDQTLAHGENKVSFTLENGLQVDVRLLEVQNFGAALLYFTGSKEHNVAL
                     RGRANDMGYTLNEYALATLKGERRIAGASEEDIYAKLKLDYIPPELRENTGEIAAAEK
                     HRLPNLVRLEDIRGDLQMHSTASDGKNSIEEMAEAARALGHGYIAITDHSKAVTVANG
                     LDEKRMAAHIKKIRAADAVARSAGATKDAPGFRVFAGAEVDILKDGSLDYSDELLAQL
                     DVVVCSVHSYMNLDRAAMTERMLAAIENPFTQIIAHPTGRLLLRRDPFDYDMEKVLDA
                     AARHGVAMECNSYPDRLDLKDVHLRMCKDRGVKVVISTDSHTTLNLPFIRYGVTMARR
                     GWLEKADVLNTLPAEQFLAALRQKPASARKKHAVRHGSR"
     gene            complement(7156..7356)
                     /locus_tag="VGR03_07305"
     CDS             complement(7156..7356)
                     /locus_tag="VGR03_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEV1994119.1"
                     /translation="MQGKVKWFNNSKGYGFIGRDDGPDVFVHYSAIVGDGYRSLQEGD
                     PVDFEIIQGPKGPQAANVSKPA"
     gene            complement(7598..9706)
                     /locus_tag="VGR03_07310"
     CDS             complement(7598..9706)
                     /locus_tag="VGR03_07310"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEV1994120.1"
                     /translation="MGPSPSPVDSTSAPALPAQGKTDRPAAILYALPVFLGAFLLFLI
                     EPMIAKVILPWFGGSAAVWSVCLVFFQSALLLGYLYAHLTSRWLSPRRQAILHIALLL
                     VCMLFLPVTPGQHWRPIPGDDPAGRILGLLAASIGLPFLLLSATSPLVQTWYARRRLA
                     AEPYHLFALSNFSSFLALLSYPFLIEPRFTARVQLGTWSALFAAFAVICSLAAWSSRS
                     SATQASAAPVSGEAAPRPGARLLWFALSACGSMLLLSITNHLIQNVAPVPLLWVLPLA
                     IYLLTFAMAFNRRSLYSRWLLVRLLAVVLGSLGYLVSEPIFIDNLPVSVPLFCAGLFV
                     CCLFCHGELYRLKPAASSLTSYYLIISLGGALGAVLVALVAPRVFRNVYEFPVTLLLT
                     ALLAGAVLWRAGLLARLFWAGAAVAMIVALVMNIRSRQEDSIVMVRNFYGALRVTEDE
                     DHAQIVRTLYNGTIVHGVQFLSPELHMRPTTYYAPATGVGIALENCCNGPKRVGVIGL
                     GAGTLAAYGQTGDLFRFYDINPQVVQIAQTSFTFLSDTPARTEIVLGDARLSLEREPP
                     QQFDVLAVDAFSGDAIPVHLLTREAVLLYLRHLKPDGILAIHTSNSYLKLAPVAKQLA
                     DSVGYSTVYINNDEDAPNIVYASEWVLITRNQAFMNLDEVSSKNDSIEVPKGVRPWTD
                     GYNSLFRILRPVRWRQSDPG"
     gene            complement(9726..10286)
                     /locus_tag="VGR03_07315"
     CDS             complement(9726..10286)
                     /locus_tag="VGR03_07315"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1994121.1"
                     /translation="MTRRAARSRSRARTVRSKTVFRGAIFDVRRDEVIEPSGVHATRE
                     VVVHSGSVVILPVLPDGRIILIRQFRYATGQFLWELVAGRKEPGESPHRAARRELLEE
                     TGFRARRCRLFLDVFPTPGYLNERMFVFLAQGLTAGAAQPEEDERITVRAFSASELDT
                     MLRRGRLRDAKSIAGILYYLRFLRRP"
     gene            complement(10283..11083)
                     /locus_tag="VGR03_07320"
     CDS             complement(10283..11083)
                     /locus_tag="VGR03_07320"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HEV1994122.1"
                     /translation="MDPKAFAREIESHIRPATFPVAVKLFKEEEPLPEKAKRPRRDMK
                     IQIATCQAIAMARRYGWTIAVGEEDLNCPLTKTAFGFSPMTEHYAEGHLACGMYVETL
                     EAGARTEAATHNLPYGQYKHLLIAPASRAEFLPDALIVYGNAAQVMRLLTGALWKRGG
                     SLTSSFTGRVDCSDEVIRTMLSGDFQVILPCYGDRVFAHTEDSEMAFSLPGAKMDELV
                     EGLAGTHKGGIRYPIPAFLRYTPQYPEHYYELERIWAAGDAPAESGRT"
     gene            11260..11541
                     /locus_tag="VGR03_07325"
     CDS             11260..11541
                     /locus_tag="VGR03_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEV1994123.1"
                     /translation="MRADNGCRHVKLIKITRTDVHVCQECVKTGDEWVHLRLCMECGH
                     VGCCDDSKNKHATKHFQKTKHPIIRSVEPGEAWTWCYIDEIAGEVAGGL"
     gene            11548..12324
                     /locus_tag="VGR03_07330"
     CDS             11548..12324
                     /locus_tag="VGR03_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEV1994124.1"
                     /translation="MPINTIIVDDEKPARDELAFLLKGFPEVQVVGQGKNGVEAVALI
                     KEHAPDLVFLDVKMPGLDGFGVLEKLVERKVKLPHVVFATAFDDFAVRAFEVNAVDYV
                     LKPFDKGRIAKAIQRARKQIETQSSAADRLEQLMSQLSAAKLAAQPTKLLVKSQQRLL
                     LVDSEDIIFASIEDGLITVMGREVEGTSNYRTLEELHATLDPDSFWRPHRSYLVNIHH
                     IKEVVPWFKSSYMLKMSDKKQAEIPVSRAQTKRLRELFKL"
     gene            12390..13244
                     /locus_tag="VGR03_07335"
     CDS             12390..13244
                     /locus_tag="VGR03_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994125.1"
                     /translation="MPQEQQIPRRLWLLGMTSRGFFQQAPRIVVVNMDILCSIVVYAG
                     FLIAMPGLVSLAWPLRFLGIASRGTGAVVLGCGVAVALIGFLLPAPETRVTAGQSQLD
                     AFLPAYQFHESHTIRIKASRERVYAAIKDVRADEILFFRTLVWIRRRGGAGPESILNP
                     RADSAVLDVALRTSFLRLAELPGQEVVLGTLVAAPRGWRPRGEPSPEGFRALRAPGFA
                     LAAMNFRLEDAGANETRVITETRIFAKDAATRRRFAVYWRVIYPGSALIRRMWLRAIK
                     KRAERPPL"
     gene            13562..14254
                     /locus_tag="VGR03_07340"
     CDS             13562..14254
                     /locus_tag="VGR03_07340"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEV1994126.1"
                     /translation="MKNKRPNAEQIWKQFEDILVPRLRLSVVDRAVYSHLLRHSRLEG
                     RPRLHFSIAWLARGARLSGGATRQAVRRLLVEGALRLVERSKTGHIVEVRLPGEIRAA
                     RDPARLPRPVSLEAADFMQSAALRQAIHARERGRCFYCLRRLTASARCLDHVVPRVRL
                     GRNSYRNLVSSCSECNSQKGERPAQDFLRSLYRERQLTAAELSTRLRALDALASGKLP
                     PPLGTAANPRLF"
     gene            complement(14711..15178)
                     /locus_tag="VGR03_07345"
     CDS             complement(14711..15178)
                     /locus_tag="VGR03_07345"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEV1994127.1"
                     /translation="MSNTGEKLLSYWKAQQLMPARGLSLQDIQAFETQHKVQMPADLR
                     DYFLQLNGLAHGWPNDQDKNGFSFWQLSRVQTVVEAKKRHRHLPVFTGMEDYFIFSDY
                     LQWSWAYAIRLTSEASGENSVVIVGKEPLQTVARSFSEFVDLYLADSSQLYGA"
     gene            complement(15210..15668)
                     /locus_tag="VGR03_07350"
     CDS             complement(15210..15668)
                     /locus_tag="VGR03_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994128.1"
                     /translation="MLKRLALALALFLVLPLPAHADNCGSLSDCYLTILAAVLVALVL
                     AVLIFFFWELIVGAAAEGALASGEIGGEIIGWGTGQAAEAVAQTEAVTQGLTTEAIEG
                     MIEEGLTREWVESQLEMYEKAAENAIKAARNTQLMPRLELMQKLLELWPK"
     gene            complement(15689..16840)
                     /locus_tag="VGR03_07355"
     CDS             complement(15689..16840)
                     /locus_tag="VGR03_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994129.1"
                     /translation="MLIGVATQYERWPNLWWLQVPAWVARISRLAAVAFADGSYLCRW
                     PKVAAFSPPLLFIFGVVAGWTHFSSGETFTFSLLLMAIMTVIASFGAGLGAWCWLGYV
                     AGDVLLYQIWHTHNDFLLTLCAASISYVLLALLLMLVPIGSENIRRRAFSFPLATLKD
                     RIVRQLASGLAQGLIQALLVFVWVHAVPTLIRPAYTWLSRSPTVPAIRPLQENGIVLV
                     VLAAVFGAVRLMLQFYALRQSSVAGLAWSLQQTLRQVGHAALWPFWIKPILPSAFSVF
                     LLAGLLDSWLDAFVLFVIFFGLNLARQVVVAKIPAWGAIILKIPWLLRFPVALLVSYG
                     ISWAIIGSMWSSTYTFRPVVVSIVLSCIVFVLFFPAPPKQSRTAQAGAR"
     gene            complement(16854..17498)
                     /locus_tag="VGR03_07360"
     CDS             complement(16854..17498)
                     /locus_tag="VGR03_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994130.1"
                     /translation="MATPQFRPWFFALAALFSGASLVSAIVFHAPLWATLVVFTTLAA
                     MGVAYRWRHTSAAGRVAVMAKVRTGMLAGLLGTCGYDLSRYTIAAVTKMSYRPFETLL
                     VFGYALAGPGVSRATAYALGTGFHLLNGICFAIAYCLFFGGRDWRLAVLWALVLEAAM
                     FSIYPTWLNLAAVKMEFTIVSFASHLAYGTILGITSQLRLAAVKRMGNHRSGFS"
     gene            17831..18544
                     /locus_tag="VGR03_07365"
     CDS             17831..18544
                     /locus_tag="VGR03_07365"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEV1994131.1"
                     /translation="MSSTYNPGRVAGLLYLSLLGAPLRLIYIPKTLFVTRNATATANN
                     IAAHETLFRLGIVSDLLTGTMAIFLMLALYRLLKGVDQDLARLMVILGSLMVTPIYFL
                     NTLNDVAALLLVRGADFLSVFDKPQRDALAMLFLRLHHGGVVANEIFWGLWLFPFGLL
                     VYRSRFLPRILGVWLIINCFAYLAVSFTGLLFPRYEAMVFNSAFPAMLGELAIMMWLL
                     IKGAKPQPLGAAALSSATG"
     gene            complement(18970..19464)
                     /locus_tag="VGR03_07370"
     CDS             complement(18970..19464)
                     /locus_tag="VGR03_07370"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1994132.1"
                     /translation="MKQIGIMLLLTATLALSLGAQKSVEHVKTYTPDAIHWGAAPNAL
                     PSGAQLAVLEGDPTKPGAYTMRLKMPDGYKIPPHHHLRREHVTVISGAFKVGMGDSFD
                     EGKMNEFAPGSFAYLEPTVHHYAKAKGETVIQLHGTGPWEIKYVNPADDPRGRAGSGK
                     TKKQ"
     gene            19693..>20338
                     /locus_tag="VGR03_07375"
     CDS             19693..>20338
                     /locus_tag="VGR03_07375"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV1994133.1"
                     /translation="MRPGVRERDRTEKFPEAEIRKLAEMGCCGMLVPEEWGGAGMDCI
                     SYALMLEEVARVDAALATILGVTNSAMTLPLLSFGNDAQRKKYLTRLARGEWIGAFCL
                     TEPQAGSDAAALQTRAVRDGGVYRLSGTKSWVSNGGAASLYLVFAKTDPEAGGKGITA
                     FLVEPAFPGFRVARLEDKMGQRTSHSAEITLNDCVVPAENRLGEEGHGLKIALAT"
BASE COUNT         4158 a         5798 c         6602 g         3780 t
ORIGIN      
        1 cctaaagtgc cgttcttcca cggcggcatc aacgtggaaa ggctgggagc gggggcgctg
       61 agccctgcgt cacgggcgtg cgagctacgt tgagcaccat ggcgaggaac gtatagtagc
      121 cggcgacgcc gaccaggtcg atgacgcccg gctcaccgta tttggcaaga acccgcgcgt
      181 aggtcgcgtc gctgacaccc tggttctggt gcagctcggt gcagaattcg tagatgtttt
      241 cttcatcggg cgacatcccc tgcggcctgc gcccttcggc cacggccttc gcgatttccg
      301 gactaacccc ggccttgagc gcgatcgggt agtgaaccgc ccactcgtat tgctgggacc
      361 agcgccgcgc ggtgatgagc atggcgaact cgttcaggtt ctccggcagc gcgctcttat
      421 aacgcaggtg gtctcccagg gctttggcgg cgagcatcac ttcggggctg cgcagaagcg
      481 ggatgaaggg gccgaacatg ggcgtgcctc gaccggcagc gaagtcggcc gccgctttct
      541 tctgggcttc cgtcatcttg tccggcggga ttggcggcag gcggtccgcg gaggtcgctt
      601 tggcgatttc cggctgtttc gcctcctgga tcgcatgtgc cccggttgcg accatggcgc
      661 aagctgcgcc ggcgcctgtc ttgatcaagg ctcttctgga tatagccatc ggctcctcca
      721 tcgttcgaac ttacggcgtg acgaatggag ggattctacc gcggagcgcg tggtttggcg
      781 agtccgtgtc tggacggcaa gggagggaga cggcgatccc acaccccgct cttatcgagc
      841 gaataaaggg ggcagccggc agaaattcct ggttaagcgt gagagaaggc ccaggtgagg
      901 cgcaccaggg cgtaaagcag ggcagcggcg atcagcagtt tgactccaac ggtggcgagt
      961 acggccatga gggcgaacag gcccaggccc atggtgagtc cactccccat gagcacaaaa
     1021 ttcctcctcc accgcgggtt atgccaccag cctttgcggg cctgtttgac ctcggctatg
     1081 gaagcctcgt cttcaaatcc ccaactccag gtctttcccc agaagcggta ccaggagaaa
     1141 aacgggcgct gatactggag ctgccccgag gtttggcctg cttctccatc gccgggttgt
     1201 gcgccggctt tgagcgacca ataaaccccg ccgcccatga agaccaggaa acccaggatt
     1261 gtgacggcca gcgtggccgc cacgaggggc cggcggtgct gctccgtgaa gctatcggct
     1321 cgtttcaggg cagggacacg ttctgcccca gcgatgataa tcaccgccaa cgccaccgtg
     1381 gcgaaccact gtacgtatcc cttcatgcgt gttccacgga taagccaccg gccgccgcga
     1441 tccggggtcg cgctggagga gctgcaagaa acggcacggc tttccccgcc gaatctcgac
     1501 ggggtggtct cgcgacccgc cagaaagcgg cgggccttgc ggcctgcggc gcgatcccgt
     1561 cccggctgat ttgagctgct ccaaatgcga ggcgagtagt gtataatgaa aaaggacggg
     1621 ttcgggagaa gcatgacgtg ccggggggcc cgccgcgaga gtagggacga tcagaataag
     1681 caccgcaaag agctcctggg gcatttcgcc atctgtgaac tctacgcatt gtttttcaag
     1741 ttatccgaaa cggcgtccag gtttgctggc tcagcctggg tatgttcctg aggaaggccc
     1801 ggccaagtca ggcagtggtt ggggggagga gaagctttgt cgctcgtgac tgaagaaaag
     1861 gccgaccagg tccgcaacct catcaatcta ggcagggaac ggggttacct tctttatgac
     1921 gaagtgaagg aggtcccttc gggcgaggcg caatcgccgg aggagatcga cagcctggtc
     1981 tccgcgttgg agcgctacgg catcgacatc tacgaagacg tttcggcggc gaaggctgcg
     2041 cacgcagtac atgatgccgc tgaatccacc gaggtcgagg cgaggaaaga ggttgccgaa
     2101 agcgaagagg ttgagctcaa cctgacgccg ggcatcgtcg agaagaacag caaccccgtg
     2161 gggatgtatc tgcgggaaat gggcaccgtg ccgctgttga cgcgcgaagg cgaggtggcc
     2221 atcgcccagc gcatcgagcg cgggcacctg ctggtgctta agacaatctc tcgctcgccg
     2281 attgtgctga aggcgctgct ggccatcggc gaagagcttc gcaagggtac ccgctcgatc
     2341 aaggaatttg tgcacttcag cgcggaagaa ctgacggaaa gagatattga gagcaagacc
     2401 cgccacacac tgcggatcat cgcgaaaatc gagaagcttt acggtgtggg gctcaaacaa
     2461 gcggcccggc tggagaagct gccgaaatcg cggaagcgcg cttacctgcg cgcccggcgc
     2521 cagttagcgc ggacacgggt cgccatgtcg ctccaggtgc gcgcgatcga tttccacccg
     2581 cttcagaaga agcgcctgat taacaagatg cggcacacag tcgaacacct gcattggctg
     2641 gagcgcgagg ccgcgcggct ggaacgccgt gccaccgcct cccgcggcga tgccgccgcc
     2701 gaagcgcgta aggagctgcg tgcgcgccgc tcggagctaa gagagatcga ggaggcgagc
     2761 gaggtcgggc tgacagtcct gaagcgcagc ctggccttgg ttcagcgcgg cgaggcggaa
     2821 gccgaacaag ggaaaaagga gctgaccgag gcaaacctcc gcctggttgt ttccatcgcc
     2881 aagaagtaca cgaaccgggg cctggagttt ctcgacctga tccaggaggg caatatcggg
     2941 ctgatgacgg gggccgacaa gttcgagtgg cgccgcggat acaagttctc cacctatgcc
     3001 acctggtgga ttcggcaagc cgtcacccgc gccatcgccg ataaggcgcg cacgatccgc
     3061 gtcccggtgc acatgatcga ggcgattaac aaacaggtcc gtaccagccg gcaactggtg
     3121 caggaactgg gccgcgagcc cacggcggaa gagatcgcca aacgcatgga catcgctgtc
     3181 gacaaggtgc gcaaaaccaa gaagatcgcg cagcagcccc tctcgatcga gacgcccatc
     3241 ggagaagagg gagattcgca cctgggcgat ttcatcgaag acaaggccgt cgtgtcgcct
     3301 gcggacgcgg tcatcaccct caatctgaag gaacagacag cgtcactgct gaagacgctc
     3361 acgccgcgcg aggagcgcat catcaagatg cgctttggcc tggaggacgg cagcgagcac
     3421 acgctcgagg aagtgggaca gtcgttcgcg gtgacccgcg agcgcatccg gcagatcgaa
     3481 gcgaaggccc tatgcaagct gcgtcatccg tcgcgctcga gccgtttccg ggtctttctc
     3541 gaaggcccct cgtgagaatc attcccggtt gccgctccgg ccgctattcg agacgacgcg
     3601 ccgccggctg tgcacgcagt taaggagagg aagaatgaag aatctttttg tcggcaacat
     3661 gagtttccag acgacggaaa gcgatttgca ggaaatgttt aaagcgttcg gacaggtcac
     3721 gcgcgtccac gtggcgatcg accgggagac cggccgggcc cgcggctttg cttttgtgga
     3781 aatgcccaat gacgaagagg cggccaaggc ggttgccgct ctcaatggaa aagaggtcgg
     3841 tgggcggagc ctaaaggtga atgaagcgcg ccccaagacg gagcggagcg cgccgcggag
     3901 cagcggcggt ggcggccgca accgcggcgg attctcaaac gaagactatc gcgaatccgc
     3961 gcgccagccg cgggaacccc gctggtagcg tcagaggatg cctgagccaa tcgcggcagg
     4021 gtgagcgccg cgtcccgaat tcaaggagag cccatgacag tcacgttcca aaagcgccag
     4081 aaggaaatga agagactgga aaaacagcgc gccaaggctg aacggcgggc gcagaagaaa
     4141 ctcgagaagc gcgcgagaag cgaagcgccc aatgaaaccg ggctcggtga aaccgccctt
     4201 ggcgaaaccg agcttggtga aaccgcgctt ggtgcagccg cggcagaacc ctccacggca
     4261 gagccgaaga tttgattgcg ggcggtttca ctctggccgg caacagggaa ctccgatcca
     4321 gctcttgtag gttcgttcgc cgggcgcacg agtaaccacg ggaacggggg agccgctcag
     4381 cctccggcac tcaaactggg gcagggtttt tcagcaccct gccaattcag acctattcca
     4441 gcgttgctat cttcttgaga tcaattttgc cggcaaaggc ggacacgaca gcctggcgat
     4501 tttcgccctt gtcccaggct ggcccgccct ggtaggccac gatctgaacg taagtgccgg
     4561 ctctgggcaa aatatcaaga gaatgggtct cattgccggg cagcgggaag gactcgtacg
     4621 caggaaagcc cccgacctcc ttcctttcct tgaactgggc cttgttctcg atggacatct
     4681 gctggagctt cttctggtcg gcgccttttt cggccagcag gacctgcacg gtgacccgct
     4741 ttgctcccat cccggccgtc gcgccggtgg gaacgtaaga ccgggtcgag ctatggccca
     4801 ccccggagac gtcgcggttg gcggtgccct cggccgtcca gcccgccgga gcgtccggga
     4861 gcaacggcgc caacttgcca gacttgccgc agcccagtag caaggcagag cacgcaacca
     4921 acagtatcca acgggtagcc ttcatcacat ctcctccacg ctgctgcttg attccgcgcg
     4981 actgttttgc tgaccttatc ttttattgcg ttagggtctt tcttggcgca gtagttttac
     5041 cgccgtcatg gattgaaacg caagcagaga ccccgtcctt cgcgtgtgga cgatggatcc
     5101 aggctctgcc ccttttcttg aggacggtga cggagcgagg agagcacacc cggacttcat
     5161 cgcggagtta agcagcacgc ggagccccga gttcgtcttg gcgcattcgg ggctaacgcg
     5221 acccatgcct gactgcgtgt tttttgcggg cggaggcggg cttctgccgt agtgcggcga
     5281 ggaattgttc ggcggggagg gtattgagga cgtcggcctt ttcgagccag ccgcggcgag
     5341 ccatggtgac gccgtagcgg atgaagggga ggttgagggt ggtgtgggag tccgtggaga
     5401 tgacgacttt gacgccgcga tccttgcaca tgcgcaggtg gacgtccttg aggtcgaggc
     5461 gatcggggta ggagttgcac tccatggcga cgccgtgccg ggcggcggca tcaaggacct
     5521 tttccatgtc gtagtcgaag gggtcgcggc ggagaaggag gcgccccgtg ggatgggcga
     5581 tgatctgcgt gaaggggttt tcgatggcgg caagcatgcg ctcggtcatg gcagcgcggt
     5641 cgagattcat ataggagtgg acggagcaaa cgacgacgtc gagctgggcg aggagttcgt
     5701 cgctgtagtc gagcgagccg tccttgagga tgtccacttc ggcgccggcg aagacgcgga
     5761 agccaggggc atccttagtt gcgcccgcag agcgggcaac ggcatcggcg gcgcgaatct
     5821 tcttgatgtg cgcagccatg cgcttctcat cgaggccgtt ggcgacggtg acggctttgc
     5881 tgtgatcggt gatggcgatg tagccgtggc cgagggcgcg ggcggcttcg gccatctcct
     5941 cgatggagtt ttttccgtcg ctggcggtcg agtgcatctg gaggtcgccc cggatgtctt
     6001 cgaggcggac aaggttcggg agacggtgct tttcggcggc ggcgatttcg ccggtgttct
     6061 cgcggagttc gggagggatg tagtcgagct tcagcttggc gtagatatct tcttcagaag
     6121 cgccggcgat gcgcctttcg cccttgagcg tggcgagggc gtattcgttc agggtgtagc
     6181 ccatgtcgtt ggcgcggccg cgcagggcga cgttgtgctc cttcgagccg gtgaagtaga
     6241 ggagggcggc gccaaagttc tggacttcga gaaggcggac gtccacctgc aagccgtttt
     6301 cgagggtgaa gctgaccttg ttctcgccgt gggcgagcgt ctggtcaatt ccggggaatt
     6361 tcaggatgtg cgcggcgaga gagtccacat gcttttgcga ggtgtggccg ggggccagcg
     6421 tgacgagcag atcgaggtca cccacggttt ccttgccgcg gcggagcgag ccggcgggca
     6481 tgatggattc gacggtcttg ccggcctttt cgatgtgcgc gacgagctgt tgtgcggccg
     6541 cctcggcgac gttgaggagg aagcggccgg aagacttctt gaagacctcg acggctttga
     6601 ggatgttctg ctcgctcttt tcaccgaagc cagcgaggtc gcggagcttg ccttcgcggg
     6661 ccagtttttc gacttcggcg actgtgcccg cgcgaaaggt tttccagagc agcgcgacct
     6721 tcttgggacc gagcgattgc agggtgagaa gatcgagaag cgtggcggga tatttcttga
     6781 ggagttttcg acgcaagccg tagtcgccct tggcgacgat ttcctggagg tgcgctgcca
     6841 tgcgctcgcc gatgccgggc agggcttcga gtttttcggg ctgctgagcg agggcggcga
     6901 tggattcggg gagggaatcg ataagctccg cggccttttc atagctgcgg tagcggccga
     6961 tgatggcgcc atctatctcc agaagctgcg ccgtttcgcg gagaatgcgg gcaacctgtt
     7021 tgttgtccat gggtgaatta tacggcggac ggcatggcgt ctgcagggga aggaggagga
     7081 aggagggcgg agagaggaag gctgcaggaa gaggccgagt cctcctccgg gcaatgggct
     7141 aatcgtgaga gcagctcagg ctggtttcga gacgtttgcg gcctgcggtc ctttagggcc
     7201 ctggataatc tcgaaatcga cggggtctcc ttcctggagc gagcggtaac cgtcgccaac
     7261 gattgcggaa taatggacga acacatcggg accgtcatcg cggccgatga agccgtagcc
     7321 ttttgaattg ttgaaccatt tcactttccc ttgcactgcg ttctcctccc ggtgggacaa
     7381 caccccagat ttattcagac gatgcgagca atccgccccc acgcgcattg gactgcaact
     7441 tccggcagtg taccaaggcg ggaatcgtgg gctgttgagc gacgtctagt agggaaatcg
     7501 gtaaagccga caaattggca ggttacctat acatgacggt aaagaccaag taacccagtc
     7561 tgcgatcgcg attgagaggg cagggcattc ttcgagatta gccggggtca gattgccgcc
     7621 aacgcacggg tctaagaatc cgaaagaggc tgttgtagcc gtccgtccag gggcgaactc
     7681 ctttggggac ttcgatcgag tcgttcttgg aagagacttc atcgagattc ataaaggcct
     7741 ggttgcgggt aatgagcacc cattccgaag cgtaaacgat attgggagcg tcctcatcgt
     7801 tgttgatgta tacggtggaa tagcccaccg aatcggcgag ttgtttggcg accggcgcga
     7861 gcttgaggta tgagttcgac gtatggatgg cgagaatgcc gtccggcttg aggtgacgga
     7921 gatagaggag gacggcttcc ctggtgagga gatgaacggg aatggcgtcg ccggagaagg
     7981 cgtctacggc gagaacgtca aattgctgcg gaggctcgcg ctcgagggag aggcgggcat
     8041 ctccgaggac gatctcggtc ctggcggggg tgtcgctcag aaaggtgaag gaggtctgag
     8101 cgatttggac aacttgcggg ttgatgtcat agaagcgaaa gaggtcgccg gtctggccgt
     8161 aagccgccag cgtgccggcg ccaaggccga ttacgccgac ccgctttggg ccgttgcagc
     8221 aattctccag ggcgatgccg acgccggtgg cgggggcgta atacgttgtg ggccgcatgt
     8281 ggagctccgg cgaaaggaac tggacgccgt gcacaatcgt gccgttgtag agggtgcgga
     8341 cgatctgggc atggtcttcg tcctcggtga cgcggagcgc accgtagaag ttccgcacca
     8401 tgacgatcga gtcctcctgg cgggagcgga tgttcatgac gagggcgacg atcatcgcaa
     8461 cggccgcgcc ggcccagaac aggcgcgcga ggaggcccgc gcgccagagg accgcgccgg
     8521 cgagaagcgc cgtgaggagc agagtcacgg ggaactcgta tacgttgcgg aaaacgcgcg
     8581 gcgcgaccag agccaccaac accgcaccca gcgcgccgcc gagtgaaatg atcaggtagt
     8641 aggaggtgag gctgctggcg gccggcttca ggcggtagag ctcgccgtga cagaacaggc
     8701 agcagacgaa cagtccggcg cagaagaggg gaacgctgac cggcaggttg tcgatgaata
     8761 ttggttctga gacgaggtag cccagggagc cgagcaccac ggcgagcaga cggaccagaa
     8821 gccagcgcga gtaaaggctg cggcggttga acgccatggc aaaggtgagg aggtaaatgg
     8881 ccaatgggag aacccacagg agcggaacgg gggcgacgtt ctggatgagg tgattggtga
     8941 tcgaaagcag gagcatggag ccgcacgccg agagagcaaa ccacaggaga cgcgcgccgg
     9001 gccggggtgc cgcttccccg gatacgggtg cggcggaggc ctgggtggcc gacgaacggc
     9061 tgctccacgc ggcgagggag caaatcaccg caaacgcagc aaacagggcc gaccaggtgc
     9121 ccagttgaac gcgggccgtg aagcgcggct cgatgaggaa gggatagctc aatagggcga
     9181 ggaaggagga gaaatttgac agcgcgaaga gatgataggg ctctgcagcg agccggcggc
     9241 gcgcgtacca ggtttgcacg agagggctgg tggcgctgag caggaggaag ggaaggccga
     9301 tggatgcggc gagcaggcca aggatgcgac cggccgggtc gtccccgggg atggggcgcc
     9361 aatgttgtcc gggcgtgacg ggcaggaaaa gcatgcagac cagcaacagc gcaatgtgca
     9421 agatcgcctg ccgccgcggg gaaagccagc gactggtgag atgcgcatag agatagccga
     9481 ggagaagagc gctctgaaaa aagacgaggc aaaccgacca aactgccgcc gagccgccga
     9541 accaggggag gattaccttg gcgatcatcg gctcgatgag aaagagaagg aacgcgccga
     9601 ggaaaaccgg cagcgcatag agaatggcgg cagggcgatc agttttgccc tgtgccggca
     9661 aagcaggcgc ggaggtggag tccactgggg agggcgaagg gcccatggaa aaagtatcca
     9721 ctggctcagg ggcggcggag gaacctgaga tagtacagga ttccggcaat ggatttggca
     9781 tcacgcaggc ggccgcggcg gagcatcgta tcgagctcgg aagcagaaaa ggcgcggacg
     9841 gtgatgcgct catcttcttc gggttgcgcc gcgcccgcgg tcagaccctg ggcgagaaac
     9901 acgaacatgc gctcattcaa gtagccaggc gtagggaaga cgtcgagaaa aaggcggcat
     9961 cggcgggcgc ggaagccggt ttcctcgagc agctcgcggc gggcggcgcg atgggggctt
    10021 tctccgggct ccttgcggcc ggcgacaagc tcccagagga actggccggt cgcgtagcga
    10081 aactggcgaa tgagaatgat gcggccgtcg ggcagcacgg gcaggatgac gaccgagcct
    10141 gagtgcacga cgacttcgcg cgtggcgtgg actccggacg gttcgatgac ctcatcgcgg
    10201 cggacgtcga agattgcgcc tcggaagacg gttttgctgc gaactgtgcg ggctctcgag
    10261 cgggaacgcg cggcgcgacg ggtcatgtgc ggccgctctc ggcgggggca tcgcccgctg
    10321 cccagatgcg ctcaagctcg tagtagtgtt ccgggtactg cggcgtgtag cgcagaaacg
    10381 caggaatcgg atagcggata ccgcccttat gcgtaccggc gaggccttcg acgagctcgt
    10441 ccatcttcgc gccgggaagc gagaaggcca tctccgagtc ttcggtgtgg gcgaagacgc
    10501 ggtcgccgta acagggcagg atgacctgga agtcgccgct gagcatggtg cggatgactt
    10561 cgtcggagca atccacgcgg ccggtgaacg agctggttag cgagccgccg cgcttccaga
    10621 gggctccggt gagcaggcgc atgacctgag cggcgttgcc ataaacgatg agagcgtcgg
    10681 ggaggaattc cgcgcgggag gcgggcgcga tgagcagatg tttgtactgg ccgtagggca
    10741 ggttgtgggt cgcggcttcg gtgcgcgcgc cggcctccag cgtttcaacg tacatcccgc
    10801 aggcgagatg gccctcggcg tagtgttccg tcatgggcga gaagccgaaa gcagttttcg
    10861 taagcgggca attgaggtcc tcttcgccga cggcgatcgt ccagccatat cggcgagcca
    10921 tggcgatggc ctggcaggtg gcgatctgga tcttcatgtc gcggcggggg cgtttggcct
    10981 tctcgggaag aggctcctcc tctttgaaaa gtttcacggc gacggggaaa gtcgccgggc
    11041 ggatatgaga ttcgatttca cgggcgaacg ctttggggtc catgggcgcc tccagcgaga
    11101 aacatgataa ccggaaagaa gggaatcgcc agagggaccg aacagtccgt agaaggagac
    11161 cggggaaagc acagcctgag tgccgtgaac cgcgcaggtg aattgccagc gtgagcgggg
    11221 agcgcctata ctggcacaga gcaagcggga aacgggaaaa tgcgagccga caacggttgc
    11281 cgtcatgtga agctgatcaa aatcacccga acggacgtgc acgtctgcca ggagtgcgtc
    11341 aagaccgggg acgagtgggt ccatctgcgc ttgtgcatgg aatgcggtca tgtgggctgc
    11401 tgtgatgact cgaagaacaa gcacgcgacg aaacattttc agaaaaccaa gcaccccatc
    11461 atccgctccg tcgaacccgg cgaggcgtgg acgtggtgtt acatcgatga gattgcgggc
    11521 gaagtcgctg gagggctgtg acggcaaatg ccgatcaaca ccatcatcgt ggatgacgag
    11581 aagccggcgc gggacgagct ggcgttcctg ctgaagggtt ttcccgaagt gcaggtcgtc
    11641 gggcagggca agaacggcgt cgaggccgtg gcgctgatca aggaacacgc ccccgacctg
    11701 gtgtttctcg acgtgaagat gccggggctg gacggcttcg gcgtgctgga aaaactcgtc
    11761 gaacgcaaag tgaagctgcc gcacgtcgtg tttgccacgg cgtttgacga cttcgcggta
    11821 cgggcgttcg aggtgaacgc cgtggattac gtgctgaagc cgttcgacaa ggggagaatc
    11881 gccaaggcca tccagcgcgc gcggaagcag atcgagacgc agtccagtgc ggccgacagg
    11941 ctcgagcagc tcatgagcca gctcagcgcg gcgaaactgg cggcgcagcc cacgaagcta
    12001 ctggtcaagt cgcagcagag actgctgctg gtggattccg aggacatcat ttttgcttcg
    12061 attgaggacg ggctgatcac ggtgatgggg cgcgaagtcg aggggacttc gaactaccgc
    12121 acgctcgagg agctgcacgc gacgctcgat ccggattcct tctggcggcc gcaccgctca
    12181 tacctcgtga acatccacca catcaaggaa gtcgtgccgt ggttcaagtc gagctacatg
    12241 ctgaagatga gcgacaagaa gcaggcggag attccagtga gccgggcgca gacgaagagg
    12301 ctgagggaac tgttcaagct gtaggagctt gttgaaaaag tgcctttggg tgtcattccg
    12361 agcaaagcga ggaatctgct tttcgcgaaa tgccccaaga acagcagatt cctcgtcgcc
    12421 tttggctcct cggaatgacc tcacgagggt ttttccaaca agctcctaga atcgttgtcg
    12481 tcaacatgga tattctctgt tccatcgtcg tttacgcggg atttctgatc gccatgcccg
    12541 gattggtttc gctcgcctgg ccgttgcggt tcctggggat cgcgagccgc ggcaccggcg
    12601 ccgtcgtgct cgggtgcggc gtggcggtgg cgttgatcgg attcctcctt cctgcacccg
    12661 aaaccagggt cacggcgggg caatcgcagc tcgatgcatt cttgcccgcg taccagttcc
    12721 atgagtcgca cacaattcgg ataaaggcct cccgcgagcg cgtttatgcg gccatcaagg
    12781 acgtgcgcgc ggatgagatt cttttttttc gaacgctggt gtggattcgg cgacgcggcg
    12841 gggcggggcc cgaaagcatc ctgaatccgc gcgcggactc ggccgttctg gatgtggccc
    12901 tgcgaaccag ttttctgcgg ctggcggagc tgccgggaca ggaggttgtg ctcggaacgc
    12961 ttgtggcggc tccgcgggga tggcggccca ggggagagcc ttcgccggag ggtttccggg
    13021 cgctgcgtgc acccggcttc gccctggcag cgatgaattt ccggctggag gatgcgggtg
    13081 cgaacgaaac tcgggtcatc acggagacgc gcatcttcgc gaaggacgct gcgacgcggc
    13141 ggagattcgc tgtctattgg cgtgtgattt atccgggaag cgcgctgatc cggaggatgt
    13201 ggctgcgggc catcaaaaag cgggccgaaa ggccgccgct gtagctcagg cctttaggcc
    13261 tgaggctttt cctccgcgtt gccacaacct cctgcaaaac tccgcggtgt tgccgggggc
    13321 tctggcaacc gcgctgacgc tgtaggacag cgccagaaat atcgcttgca aagcgccttc
    13381 aatctgttat tatatcttcg ggcgaattgt ggcttccaca gttcgccttt ttctttttcc
    13441 ccggtcccgg ttcgctgcta ctgcgcaccg gcttctgttc cggcacttcc gtcttcctgt
    13501 agtcatccca aatcctcgtc cgccgagaac tccgctcgcg ctgaccctct ggacttgcac
    13561 catgaaaaac aaaagaccca acgccgaaca aatctggaaa cagttcgaag acatcctcgt
    13621 gccccggctg cgcctgtccg tcgtcgaccg cgccgtctat tctcacctcc tgcggcacag
    13681 tcgcctcgaa ggccgtcccc ggctccactt ctccattgcc tggctcgccc gtggcgcccg
    13741 gctctcaggc ggggccacgc gccaagcagt gcgccgcctc ctcgtcgagg gcgccttgcg
    13801 cctcgtggag cgcagcaaga ccggtcacat cgtcgaagtg cgcctgcccg gggagattcg
    13861 cgcggctcgc gacccggccc ggctgccccg ccccgtctcc ctcgaagccg ctgatttcat
    13921 gcaatccgca gccttgcgcc aggccatcca cgcccgcgag cgcggccgct gcttctattg
    13981 tctccgtcgg cttaccgcct cagcgcggtg cctcgaccat gttgtgccgc gagtccgcct
    14041 gggacgcaat tcctaccgca acctcgtctc ctcctgttcg gagtgcaact cacaaaaggg
    14101 cgagaggccc gcgcaggatt tcctccgcag cctctatcgc gagcgccaat tgaccgccgc
    14161 cgagctttct actcgcctcc gcgccctcga cgccctcgcc tccggcaagc tcccgcctcc
    14221 gctcggcaca gccgccaatc cgcgattgtt ctgatctcgg gagcttccgc acgcactgtg
    14281 cggaaaaacc aggcgtcgcc ataatttagt aaaattggtc ggggcgagtg gatttgaacc
    14341 tccgacctac cggtcccgaa tcaaatacca atacttattg gaacctgtta gaatctgttg
    14401 tttctaactg attgaattga atctgttgga gatcgttcgt tgagagctgt tgttttttgt
    14461 tgaagtcgga ggctttcgca gccgcaaatt tgcctacacc ttgtctcgtt ctaggggttc
    14521 agcccacgac caccaccgca ccttattcgt tggttccctc taagccgatt gaaactcttt
    14581 caagaaacaa gagggtaagc tgccccaaaa ggtaaacttt ggggttcttg caagttattg
    14641 aatcgattgc tgtacaacat tttggcgaac agcaaccggt gacggctcgc tatcccggcg
    14701 gcgttttttc tcaagcccca tagagttgtg aggagtctgc caggtaaaga tccacaaatt
    14761 ccgagaacga gcgggccacg gtttgcagcg gttctttgcc aactatgacc accgagtttt
    14821 ctccgctagc ttcacttgtc agacgaatgg catacgccca ggaccactgc aagtaatcag
    14881 aaaaaatgaa gtagtcttcc atgccggtga acacaggcag atgtcggtgc cgtttctttg
    14941 cttccacgac cgtctgcacc cgagatagct gccaaaagga aaacccgttc ttgtcctgat
    15001 cattgggcca tccgtgggcc agtccgttga gttgcaggaa atagtcacgc agatcggcag
    15061 gcatttgcac tttgtgctgg gtttcaaatg cctgaatgtc ctgcaaagac agacctctag
    15121 ccggcatcag ttgctgggcc ttccagtatg aaagcagctt ttctcccgtg ttgctcattt
    15181 cggtttccta tctgcgccgg agttagcgat cactttggcc acagctccaa aagtttttgc
    15241 atcagttcca gccggggcat caactgggtg ttacgggcgg ccttgatagc gttttcagcg
    15301 gccttttcat acatttccag ctggctctca acccattcac gcgtgaggcc ctcttcaatc
    15361 attccctcaa ttgcctccgt agttaaaccc tgggtaacgg cctcagtctg agcgactgct
    15421 tccgcggcct gtccggtacc ccacccgatg atttcgccgc caatctctcc cgacgcaagg
    15481 gcgccttcgg cagccgctcc gacgattagc tcccagaaga agaaaatcag gaccgcaagc
    15541 accaatgcca ccagaaccgc cgccaggatg gtcaggtagc aatctgacag gctgccgcag
    15601 ttgtccgcat gggcggggag gggaaggacc agaaagaggg ccagggccag ggccaggcgc
    15661 ttcagcattc gtcttgtccg tgaggctgtc atcgcgcgcc tgcctgtgct gtccggctct
    15721 gcttcggcgg cgcggggaaa aacaatacga agacaatgca ggagagaacg atggacacca
    15781 ccaccggacg aaaggtataa gtgctggacc acatgctgcc aatgatggcc caggaaatgc
    15841 cgtagctcac cagcagtgcc actggaaacc tcagcagcca ggggattttg agaatgattg
    15901 cgccccatgc cggaattttg gctaccacca cctggcgggc caggttgagg ccaaaaaaga
    15961 tgacaaagag cacaaaggcg tcaagccagg aatcgagcaa gccagccagc aaaaagaccg
    16021 agaaggctga tggcagaatc ggcttgatcc agaacggcca aagcgcagca tggccaactt
    16081 ggcgcaacgt ctgctgcaaa ctccaggcca ggccggccac tgaggattgc cgcaaggcat
    16141 aaaactgcaa catcaggcgc actgcgccaa agacagccgc aagcaccacc aagactatgc
    16201 cattttcctg aagagggcga atcgctggga ccgttggcga cctggagagc cacgtataag
    16261 caggacgaat cagggtagga acagcgtgca cccaaacaaa caccagcagc gcctgtataa
    16321 gtccctgggc cagaccgctg gcaagctggc gaactattct gtccttcaga gtagcgaggg
    16381 ggaaggaaaa ggcccggcgg cggatgtttt cagagccaat cgggacgagc attagcagca
    16441 gggccagaag aacgtaagag atcgaggcgg cgcagagagt cagcagaaaa tcattgtggg
    16501 tgtgccagat ctgatacagc agaacgtcgc ccgcaacgta tccgagccag caccaagccc
    16561 ccagaccggc gccaaagctc gcgatgaccg tcatgatggc catcaagagc agggagaatg
    16621 tgaaggtctc gccggaggag aaatgggtcc agccggccac cacgccaaag atgaaaagaa
    16681 gcggcggaga aaaagcggca accttcggcc agcgacaaag ataggatccg tctgcgaacg
    16741 ccaccgctgc aagccgcgaa atcctggcaa cccacgccgg cacttgaagc caccataggt
    16801 tcggccatcg ttcgtattgt gtggcgacgc cgataagcaa ctccccaacc gtctcacgag
    16861 aaaccggaac ggtgattgcc catgcgctta acagcagcta atcttaactg ggatgttata
    16921 ccaagaatcg taccataggc caggtggctg gcgaacgaaa caatggtaaa ctccattttg
    16981 accgccgcca gattcagcca cgtagggtag atggaaaaca tggcggcttc cagaactagg
    17041 gcccaaagga cggcgagcct ccaatctctc ccgccaaaga aaaggcaata agcgatggcg
    17101 aaacaaattc cgttcagcag atggaagccc gttcccaggg cataggcagt ggcccgtgat
    17161 acgccgggtc cggcaagcgc gtagccgaac accagcaacg tttcgaaggg gcgatagctc
    17221 atctttgtca cagcggcgat cgtgtaccgc gacaggtcat agccgcacgt gcccagcaag
    17281 ccggccagca ttcctgtccg gaccttggcc atcacagcca cgcgcccggc cgcagaggtg
    17341 tgccgccaac gataggcaac ccccatggct gccagcgtgg tgaagacaac aagggtcgcc
    17401 cacagcgggg cgtgaaaaac aatggcggaa actaacgatg ctccgctaaa cagtgcggct
    17461 agggcaaaaa accatgggcg gaattgtggc gttgccatgc tcaatggccc gctggccatt
    17521 gtaactcgga atttcgtctt tgaatgtgat gcgccttgac ggagcagtgg cgggtggtag
    17581 agcgaattga actcctggct gtgcaaattc aggaagccaa cgccctccgt tggagggcga
    17641 cttcgcagcc gccaacccgc tcccccgcat cggccgcttg tcccgatgaa tcgaggcctc
    17701 gcctcggcac atcggggccc gccgagtgcc cacaccagac gcgtttgctc gtatggcgaa
    17761 actttttgat agcctgcggc gtatctccgg ctagaccctt acactaggcc cgttcaacga
    17821 gggagggaac atgagctcga cctacaatcc agggagagtc gcagggttgc tctatctatc
    17881 gctgttaggc gctcccctcc gcctcatcta tattcccaaa actttgttcg tgaccaggaa
    17941 cgcgaccgcg acggccaaca atatcgccgc gcacgagacg ctcttccgtc ttggcatcgt
    18001 cagcgacctg ctcaccggga ccatggctat cttcctcatg ctggctctgt accggctgct
    18061 caagggagtg gaccaggacc tcgctcgact gatggtgatc ctggggagtc tgatggtgac
    18121 gccaatctac ttcctcaaca cgctgaacga cgtcgctgcc ctcctgctcg tgcgtggcgc
    18181 cgatttcctc tccgtattcg acaaaccgca gcgcgacgcc cttgccatgc tgttcctccg
    18241 tctgcatcac ggtggcgtcg ttgccaacga gatcttttgg ggcctatggc tctttccctt
    18301 cggcctgctg gtgtaccggt cacgctttct tccccggatc ctgggcgtct ggctgatcat
    18361 caactgcttc gcctatctgg ccgtcagctt tacgggctta ctgttcccgc gatacgaggc
    18421 gatggtcttt aacagcgcct tccccgccat gctcggagaa ctggcgatca tgatgtggct
    18481 tctgatcaag ggcgccaagc cgcaaccgct gggcgccgca gcattgtcgt cggcgactgg
    18541 ttaggctgca tcacttgcgt ttgacaactc cggcgttagg cgtagtgtag cccctcgccg
    18601 gccgtgctct cccatctgcc tgaaaagcat ccacggcgca agtcgcgtcg gaaacataca
    18661 cgcgcccacg cttgagattc cccccctttc agagctgtag ctcaggcctt taggcctgag
    18721 gcttttactc cgcgttgccg caagcctccg tcaacctccc acgccgcgga gggtgtcgga
    18781 atttctcgct tataccgttg acgccgggtt ctcgcccaat gcccgtctct gggccagttt
    18841 ccgagtggcc gacgatccgg gagctatcgc gcgggtggct cgaatcgaaa tcccgaatac
    18901 gggcccgagt acggccactt ctaacccggc cagcgtggaa cggctatcag gggtgattcc
    18961 ggaaatactc tattgcttct tggttttgcc cgacccagcc cgaccccgcg gatcgtctgc
    19021 cggattaacg tatttaatct cccacgggcc agtcccgtgt aactggatca ccgtttcgcc
    19081 cttggccttc gcatagtgat gcaccgttgg ttcaaggtac gcgaagcttc cgggggcaaa
    19141 ttcgttcatc tttccttcgt cgaagctgtc acccatgcct accttaaacg ctcctgagat
    19201 caccgtcacg tgctcgcgac gaagatggtg gtggggcggg atcttgtatc cgtccggcat
    19261 tttcaaccgc attgtgtagg cccccggctt tgtcggatcg ccttcgagaa ctgctaattg
    19321 agctccagaa ggtagagcat tcggggctgc accccagtgg atcgcatcgg gtgtataagt
    19381 cttgacatgc tcgactgatt tctgagcacc aagactcaga gccaacgtag cagtaagcaa
    19441 aagcattatt ccaatttgct tcattagttc acctccaaga aggctccatt gtacgcgagt
    19501 gacggcgtac tcggaagtaa tgaacttagc cggatagcgc gctcatcgga aactgaccga
    19561 ccatccaccg cctgccttct gcttctcggc tggctgtgct agcatcgcct gacacctagc
    19621 gcgatggact tttcgctcaa agaccaccaa aaactgattc gggataccgt tcggcaattc
    19681 atggaggccg aggtgcgtcc aggagttcgc gaacgcgacc gcacggagaa attccccgag
    19741 gcggaaatcc gcaagctggc ggagatgggc tgctgcggaa tgctggtgcc ggaagagtgg
    19801 ggcggcgcgg ggatggactg catctcctac gcgctgatgc tcgaggaagt ggcgcgcgtg
    19861 gacgcggcgc tggcgactat cctgggggtg acgaactcgg cgatgaccct gccgctgctg
    19921 tcgttcggaa acgacgcgca gagaaagaag tacctgacgc ggctggcgcg tggcgaatgg
    19981 atcggggcgt tttgcctgac cgagccgcag gcaggctcgg acgcggcggc gctccagacg
    20041 cgagccgtgc gtgacggcgg cgtgtaccgg ctgagcggaa caaaatcgtg ggtgagcaac
    20101 ggcggcgccg cgagcctcta cctcgttttc gcgaagacgg atccggaggc aggcgggaag
    20161 ggaattacgg cgtttctggt ggagccggcg tttccgggat ttcgcgtggc gcggctcgaa
    20221 gacaaaatgg ggcagaggac ttcacactcg gcggagatca cgctgaacga ttgcgtggtg
    20281 ccggcggaga accgtttggg cgaagagggg cacgggctga agatcgcgct ggcgacgc
//
LOCUS       DASYVO010000223         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_144437, whole genome shotgun sequence.
ACCESSION   DASYVO010000223 DASYVO010000000
VERSION     DASYVO010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144437"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VGR03_07380"
     CDS             complement(<1..483)
                     /locus_tag="VGR03_07380"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV1994134.1"
                     /translation="MATTTVPPSPPKKLNRIGLEIAEYRGNKTTLCAGCGHNAITERI
                     IEAFYELGVEPYRVAKLSGIGCSSKSPAYFLSSSHGFNAVHGRMPSVATGTLLANQHL
                     VAVGVSGDGDTASIGIGQFVHLMRRNLPIIYIIEDNGCYGLTKGQFSATADLGSKLKT
                     G"
     gene            complement(485..>2014)
                     /locus_tag="VGR03_07385"
     CDS             complement(485..>2014)
                     /locus_tag="VGR03_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEV1994135.1"
                     /translation="LDPDAAVVYDERLNLKRLRQDLKFYPVPFDQLVAPVCPDARLRK
                     LVRNMIYDGVVAKLLGIEMTEIQEALKKQFKKKAKAAELNWNAARAGYEYAEKNLTKA
                     DRFYLERMDETRGKIIIDGNSAAALGSVFAGVTVLTWYPITPSSSLAEMVIEYMKKYR
                     IDPATGKATFAVVQAEDELAAIGMALGAGWAGARSMTTTSGPGISLMAEFVGLGYYAE
                     IPAVIFDVQRVGPSTGLPTRTSQGNVMKSALLSHGDAKHLLLFPCSVEECFTMAVEAF
                     DIAEEFQTPVFVMSDLDLGMNNWMADPFPYPEKPMVRGKVLNADDLKRLGGFERYRDV
                     DGDGIGYRTLPGTNHPAAGYFTRGSGHNQKAVYSERASDYVDNMDRLARKFETARKRV
                     PAPIIHEASAPAFGILAYGTSHWAVIESLDQMKHEHEIEASYCRVRGFPFNDEIKKFI
                     HRHERVYVVEQNRDAQMYSLLRMEIDAEDVKKLHSIRHYNGLPIDARSVTDAIVSQEV
                     K"
BASE COUNT          405 a          568 c          589 g          452 t
ORIGIN      
        1 tcccgtcttg agttttgatc cgaggtcggc ggtagcggaa aactgaccct tggtcagccc
       61 atagcaaccg ttgtcctcaa taatgtaaat gatggggagg tttctgcgca tcaggtgcac
      121 gaactggccg atgccgatcg acgcggtgtc gccgtcaccg gagaccccga cggcgaccag
      181 gtgttgattg gcgagcagcg taccggtggc gacggagggc atgcgtccgt gcacggcatt
      241 gaagccgtgc gaggagctca ggaaataggc cgggcttttc gaagagcatc cgatgccgga
      301 aagtttggcc acgcggtaag gctcaacacc cagctcgtaa aatgcttcga taatgcgttc
      361 ggtaatcgcg ttatgcccgc aaccggcgca aagcgtcgtt ttattgccgc ggtattccgc
      421 aatctcgagc ccaattcgat ttaacttctt gggtggggaa ggcgggacgg tggtagtagc
      481 catattattt cacctcttgg gatacgatgg catcggtaac cgagcgcgcg tcaatgggca
      541 agccattgta gtggcgaatg ctgtgcagtt ttttcacatc ttcggcatcg atttccatcc
      601 gcagcaaact gtacatttgc gcgtcacgat tctgttccac tacatataca cgctcgtgcc
      661 ggtggatgaa tttcttgatt tcgtcgttga aaggaaaccc gcgcacgcgg cagtagctgg
      721 cttcaatttc gtgttcgtgc ttcatctggt caaggctctc gatcaccgcc caatggctgg
      781 tgccgtaggc aagaattccg aaagccggcg ccgacgcttc gtggataatc ggcgcgggca
      841 cgcgtttgcg ggcggtctca aacttgcggg cgaggcggtc catgttgtcc acataatcgc
      901 tggcgcgctc gctgtaaaca gctttctgat tgtggccgct gccgcgagtg aagtagccgg
      961 ccgccgggtg attggtgccc ggcagagttc ggtaaccgat tccgtcgcca tccacgtcgc
     1021 gataacgctc gaagccgccc aggcgtttca aatcgtccgc gttaagtact ttgcctcgca
     1081 ccatgggttt ttcagggtag ggaaacggat cagccatcca gttgttcatg cccaaatcaa
     1141 gatcgctcat cacaaagacg ggcgtctgga attcctccgc gatatcgaag gcctcaacgg
     1201 ccatggtaaa gcactcctcg acggagcacg gaaacaacaa aaggtgtttg gcatcgccgt
     1261 gcgaaagcag cgcgctcttc atgacatttc cttgcgaggt gcgcgtgggg aggccggtgg
     1321 aaggtcccac gcgctgcacg tcaaaaatca ccgcaggaat ttccgcgtag tacccgaggc
     1381 ccacaaactc tgccatcaat gaaatgcccg gccccgaagt ggtggtcatg gagcgcgcgc
     1441 ccgcccaccc cgctcccagc gccataccga ttgcggccag ctcgtcttcc gcctggacca
     1501 cggcgaacgt cgccttgccg gtggctggat cgatgcggta tttcttcatg tattcgatga
     1561 ccatctccgc cagggacgat gacggtgtaa tgggatacca ggtgagaaca gttacgccgg
     1621 cgaataccga cccgagggcg gccgcggaat tgccgtcaat gatgattttg ccgcgcgttt
     1681 cgtccattcg ttccagatag aagcggtcag ccttggtgag gtttttctcg gcgtactcgt
     1741 atccggctcg cgccgcattc cagtttaatt ccgccgcctt ggcttttttc ttgaattgtt
     1801 ttttcagggc ctcctggatc tccgtcatct caatgcccag cagtttcgcc accacgccat
     1861 cgtaaatcat gttacgaacc agcttgcgga ggcgggcatc ggggcacacg ggcgccacga
     1921 gctgatcgaa agggacgggg tagaacttta aatcctggcg caagcgtttc aggttcaagc
     1981 gctcgtcgta taccacggcg gcgtccggat cgag
//
LOCUS       DASYVO010000224         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_260433, whole genome shotgun sequence.
ACCESSION   DASYVO010000224 DASYVO010000000
VERSION     DASYVO010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260433"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VGR03_07390"
     CDS             <1..450
                     /locus_tag="VGR03_07390"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV1994136.1"
                     /translation="WPIVGQAFSSAHFIPVNRSAHDSAVASVEKATEALRAGQSFLIY
                     PEGTRSLDGRLQRFKKGAVVMAINAAVPIVPMVCSGAHRIMEKRSLVIHPGEIVVEFL
                     DPIDSSGYSLEQRDFLNEKIREAMAARLPPDQRPLGFPGATAEPAVS"
     gene            complement(476..1513)
                     /gene="lpxD"
                     /locus_tag="VGR03_07395"
     CDS             complement(476..1513)
                     /gene="lpxD"
                     /locus_tag="VGR03_07395"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEV1994137.1"
                     /translation="MKLSEVAQKLGCRLEGPPELEIRGVAGIEYAEAGQITFLANRRY
                     FPLLKTTRASAVLVEEGIALDREVSLPALAALRSASPYADFGKALELFYQPPRYAPGI
                     HPTAIIASSAKIGEGAHIGPYCYVDEDAEIGRNAVLHSFVTIYPGAKIGDDFFSHAHV
                     VVREYCRIGNRVVLQDGVVLGGDGFGFAKQKDGSWRKIPQAGCVVLEDDVEVQANSCV
                     DRATVGETRIGRGCKVDDLVLVGHGSRIGANTLLCGQVGLAGSTKVGSDCVLAGQVGS
                     GGHITIGDRTLITAQAGIPHDLAGDKHYSGSPCVDHKQWLKNAAALNRTPELQRRVRE
                     LESEIEKLKSR"
     gene            complement(1605..2099)
                     /locus_tag="VGR03_07400"
     CDS             complement(1605..2099)
                     /locus_tag="VGR03_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994138.1"
                     /translation="MKNRAPASTHKKVIVVLADKKPLRGYLNPGRLGQADPIDLLTPD
                     GEHIEVPLDRVRSIYFVREFSDDFEPERKAFLSRPKLDGLWVKLRYNDGETLEGVVPN
                     DLLSLLDNGLQITTPDLNSSTDRIFVPRAALTEVTVLGVVGIARRKPAAAAAAASQPR
                     LFNE"
     gene            2225..>2461
                     /locus_tag="VGR03_07405"
     CDS             2225..>2461
                     /locus_tag="VGR03_07405"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEV1994139.1"
                     /translation="MTDKKSILKSASIITLVTIASRILGYVRDQRITLLLGTSFAADA
                     YVLAYRIPNLFRRLVAEGSMTASFIPVFTSYMREK"
BASE COUNT          469 a          773 c          673 g          546 t
ORIGIN      
        1 tggccgattg tcgggcaagc tttttcctcg gctcatttca tccccgtgaa tcgcagtgcg
       61 cacgactccg ccgtcgccag cgtggagaag gctaccgaag ctctgcgcgc ggggcaatcc
      121 tttttgattt atccggaagg cacgcgcagt ctcgacggcc gccttcagcg ttttaagaag
      181 ggtgccgtgg tgatggccat caatgcggcc gtgccgatcg tcccgatggt ctgctccggc
      241 gctcaccgca tcatggaaaa acgttcgctg gtgattcacc ccggagaaat tgtggtcgaa
      301 ttccttgatc cgattgacag ctcagggtat tcgttagaac agcgggattt tctgaacgag
      361 aagatccgtg aggcgatggc cgcgcgcctg ccaccggacc agcgtcctct cggttttcca
      421 ggcgccaccg ccgaaccagc cgtatcctga tttgggggcg gcaacgcgcg tgttttcagc
      481 gcgatttcag cttctctatt tccgattcca gttcgcgaac acgcctttgc agctcgggcg
      541 ttcgatttag cgcggccgca ttcttgagcc attgcttgtg gtccacgcag ggcgaaccag
      601 agtaatgctt gtccccagca aggtcgtgcg gtattccggc ctgtgccgtg attaaggtgc
      661 ggtcaccaat ggtaatgtgt cctcccgagc cgacttgccc ggccagaacg caatcgctgc
      721 cgacctttgt cgatccggca agcccgacct ggccgcaaag caaagtgttg gcgccgatac
      781 gtgagccatg gccgacaaga accaggtcgt ccaccttgca accgcgtccg atgcgcgtct
      841 cgccgacggt ggcccggtcc acgcacgaat ttgcctgcac ctcgacgtcg tcttcgagca
      901 ccacgcaacc cgcttgcgga atcttgcgcc agctgccgtc cttctgtttt gcaaatccga
      961 agccgtctcc tccgagaact acaccatctt gcaagaccac acgattgcca atgcggcaat
     1021 attcccgcac aacgacgtgc gcgtgcgaga agaaatcatc ccctatcttc gcgcccggat
     1081 agatcgtgac gaaactgtga agcacggcat tgcgcccgat ttccgcgtct tcgtccacgt
     1141 agcagtacgg tccgatatgc gctccctcac caatcttggc gctgctcgcg atgatcgctg
     1201 tcggatgaat ccccggcgca tagcgcggtg gttgatagaa aagctctaag gctttcccga
     1261 agtccgcgta cgggcttgcg gagcgtagcg ccgccagtgc tggcagcgac acctcccgat
     1321 ccagcgcaat tccttcttcg acgagaaccg ccgaagcgcg cgtcgtcttc agcaacggaa
     1381 agtaccgccg attcgcgagg aacgtaatct gccccgcttc ggcatactcg atcccggcca
     1441 ccccgcgaat ctcgagttcc ggcgggccct ccaatcggca tcccaatttc tgcgcaactt
     1501 cagaaagttt catggagggg ctcctcaatc ctttatgggg cctttgtggg gctgtctgcg
     1561 agtctcgcac aggccaaaga gggaagtaca gacggctcgc tagcctattc gttgaaaagg
     1621 cggggttggg acgcagcggc tgcagcggcg gccggcttcc gccgcgcgat gcctacgact
     1681 ccaagaacgg tcacttccgt gagcgcggca cgcggaacaa agatacggtc cgtggaagag
     1741 ttcaggtccg gcgttgtgat ttgcaaacca ttgtccagca gcgacagcag atcgttcggc
     1801 acaacgcctt ccagggtttc tccatcgttg taacgcagct ttacccacaa tccgtccagc
     1861 tttggccggc tcagaaacgc cttgcgctcc ggttcgaagt cgtcggaaaa ctcgcgcacg
     1921 aaatagatcg atcgcactcg atccagcggt acctcaatgt gttccccatc cggcgtgagt
     1981 aggtcgatgg ggtcggcctg tcccagccgc ccggggttca ggtagccgcg caagggcttc
     2041 ttgtcggcga gcacaacgat aaccttcttg tgcgttgagg ctggggctct gtttttcaaa
     2101 tttccgcgtt tcccggtccg gaaggcccaa atttgccaat cggcaaaatg tatgttatgt
     2161 taagtgcgtt tttcctgtca acttattgtt ttcattaggg ttagggcccc cggagcagcg
     2221 ccctttgacc gataaaaaga gcatcctaaa atcggcctcg atcatcaccc tggtcactat
     2281 cgctagccgc atcctcggct acgtccgcga ccagcgcatc accctcctgc taggcacgtc
     2341 ctttgctgcc gacgcttacg ttcttgccta tcgtataccc aatctgttcc gccgcctcgt
     2401 cgccgaaggt tccatgaccg cctccttcat ccccgtcttc acctcttata tgcgggagaa
     2461 a
//
LOCUS       DASYVO010000225         5831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_75155, whole genome shotgun sequence.
ACCESSION   DASYVO010000225 DASYVO010000000
VERSION     DASYVO010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75155"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            505..1653
                     /locus_tag="VGR03_07410"
     CDS             505..1653
                     /locus_tag="VGR03_07410"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994140.1"
                     /translation="MNRPKNFRVYFLLAALAIAGLVVLLREHRPSFLRPDLHLSAYVT
                     TADGNVTVVDLVKLKAVARIAVGPGLSGIREHPTRPEVFGVSTAGGYVWIIDPRANHF
                     TGQVTARIPVGPLPYALDFSPDGNRIYTTASGNDTLLVIDAKSRAIVARAPTGSEPVL
                     AHVTPDGKTVLVVNRRNGSLGIHDAATLALRGSVSVVAQPEDVVVLPDSSLAFVLSRT
                     ERRISVVDLRRGVLVTNLELAGVPTDMLLKPDGGELYVISPEAHGLQAINTRTHEVGD
                     YMVLGSAPTRGVLSEDASLLYVSDTAAGRVTPVDIFNRRIVREAGKGFPVPAGDAPAA
                     LRFDPAENLLLVVSQGSGDLAVIRVRTNFLVTMIPVGDHPQELAVKLF"
     gene            complement(1795..3987)
                     /locus_tag="VGR03_07415"
     CDS             complement(1795..3987)
                     /locus_tag="VGR03_07415"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HEV1994141.1"
                     /translation="MKRVHLVVLWHMHQPQYRDPESRRYVLPWTRLHALKDYWGMVEV
                     LREFPNFHATFNVVPSLGVQLEEYASCSFIEPWFSLAFKNADELTREDKAEIIARAFQ
                     VNHEQLMLRWPRFVELFEWSRPAGGAQALVSFTPRDWRDLQLLSQLAWMEESWFATDD
                     VVSRLATKGKDYTEKDKSALRKKQLDFLGLVLPAYRDAAQRGQIEISTTPFYHPILPL
                     ICDSDIARVANPGTPLPRRAYRRPEDAREQLHLAREYHERVFGVKPAGLWPSEGSVSD
                     QALAIAVEEGFQWFGTDEGVLGRTLNVGFFRDGNGIPANADRLYKPWRVQLGGKSIAG
                     LFRDHHLSDLVGFVYSRMDAKEAAADLHSRMRHLGENVQGDQPLTICLFLDGENAWEY
                     YPGNGRAFLREFYGRIQGDQDFRALTASEAIAAAGEIPTTTGIFPASWINANFDVWIG
                     HAEDVAAWELLWDAREAYARAFEARKQGRSDAPTETALKEARESLLVAEGSDWCWWFG
                     PEHSTANDAEFDALYRKHLTAIYLALGQLAPEELAKPIKRRPEHAVQLAPTGWLNIKI
                     DGRDTSYFEWLGAGLYSPERRGGSMHGRVFYLHELRYGFEEGRFCVRIDPFAEALAEL
                     EDPEFRITIGGAEEVTLVVNLERGHVQGFAVEKNRVCLLNPKVVAEAAFERVLEVAIH
                     KDQINFKGQSKLRLGVALWHGGLPVDVLPAEGFLDVNLGKDNLAWPPE"
     gene            complement(4375..>5831)
                     /locus_tag="VGR03_07420"
     CDS             complement(4375..>5831)
                     /locus_tag="VGR03_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1994142.1"
                     /translation="SLILATGGAAVGTLLAWGGLKSLVALMPQNIIPAEAVIRLNTPV
                     LAFTLGVAVLTALVFGLVPALKAARKDLNEPLRDSGKGISGGFRHGRLRDALVVLEVG
                     LSLTLLVAAGLLMRSFVALRDVKLGLHPDHVLVARLPLPVERYKTADQVAGFYRPLLQ
                     RLKALPGVVDATETSTLPPYGGIPSDIEIPGKTHAEKWNAMFQLVSEGYFPVLKIQFR
                     DGRGFTEAEVNGARKLAIVNQTFVKKYLGNENPIGRQVRIARLAEFDDAVKEPMFEII
                     GLVADAKNQGLQDPVLPEIWVPYTVTGSAFRGILVRTAREPLTMMNAVQHEIWATDAN
                     VAVTLTGTLEGYISQFSFAGPRFGFFLMTIFGAIGLVLVTIGVYSVLAYTTVRRTQEI
                     GIRMALGAKGSDVQRMVIRMGLRLVGLGVGIGLIVSLAIGRVIATQLWGVSAYDPWTL
                     VCVPAVLLITGIVACWLPARRAASVDPLVALRYE"
BASE COUNT         1144 a         1916 c         1617 g         1154 t
ORIGIN      
        1 ccccttaaac tctgtcgtat catatcgtta caaaaacata ggagggggca ctgcttcacc
       61 cgcgtgctct gcgcttcgcg gagagggggc ctcaactttc caatccccac cccctattcc
      121 ctacgtcctt tcctctaact cctttccttg ccacacatcc gaaaaacaca cccataactc
      181 ctttgcttgc cacacatcca aaaacacagg gtttaaagtc ctttatttgc cacacatccg
      241 agaaagaggg ggtgggtcgg gcaaattgtt aaccagattc ccgatgaagg aatctgtcct
      301 gaagagcatc gcaacgaagg acctctcttc attcccgacc aagattccta tcccgtttac
      361 cctgagcttc gcacggagcg acactcgcga gccgaccgaa ggttccgacc cagtcggaag
      421 ggatctgttt cttctttacc tcgttacctc atggcttcgt tacttctttc cttcccgctc
      481 tcatgccaca attccgaggc ccgcatgaac cgccccaaga acttccgcgt ctacttcctg
      541 ctcgccgcgc tggccattgc cgggctggtc gtacttctgc gcgagcatcg cccttcgttc
      601 cttcggccgg acctgcatct ctccgcgtac gtcaccacgg cggacggcaa cgtcaccgtc
      661 gtcgatctcg tgaagctcaa agccgttgcg cgcatcgcgg tcggcccggg actctccggg
      721 attcgcgagc atcccacgcg ccctgaggtt ttcggcgtca gcaccgctgg cggctacgtg
      781 tggatcatcg atccacgcgc caatcacttc accgggcagg tcaccgcgcg aattcccgtg
      841 gggccgcttc cctacgcgct cgatttttcg ccggacggca accgcatcta cacgacggct
      901 tccgggaacg atacgctcct cgtgattgac gctaaatcgc gcgccatcgt cgcgcgcgca
      961 ccgacaggga gcgaacccgt gcttgcgcac gttacgccgg acggcaagac cgtcctggtg
     1021 gtgaatcgcc gcaacggctc tcttggcatt catgacgcag cgactctcgc gctacggggc
     1081 agcgtttccg tggtcgcgca gccggaagac gttgtcgtcc tgccggatag ttccctggcg
     1141 ttcgtgctga gccgcaccga gcgccgcatc tccgtcgtgg acctgcgccg cggcgttctg
     1201 gtcaccaatc tcgagttggc cggcgtgccc acggacatgc tgctgaagcc cgatggcggc
     1261 gaactctacg tgatttcgcc ggaggctcac ggcctgcagg ccatcaacac ccggactcac
     1321 gaggtgggcg actacatggt cctgggatcg gcccccactc gcggagtcct cagtgaggat
     1381 gcgagcctgc tctatgtttc ggacactgct gccggccgcg tcacgccggt ggatattttc
     1441 aaccggcgca tcgtgcgtga ggccgggaaa ggattccctg tgccggccgg ggatgctccc
     1501 gcggcgttgc gcttcgatcc cgcggaaaac ctgcttctcg tggtgagcca gggctccggg
     1561 gacctggcgg tcatacgggt gcggacgaat tttctggtga cgatgattcc ggtgggcgat
     1621 catccgcagg agctggcggt gaaattgttt taggggccgt tgggacggcg gaaaagggct
     1681 taacacagag ttcacagagg gcacggagag gaaagaaacg gaagaaacta catcaaacca
     1741 ggagagcggc ccacctcccc gacaggagtc ggcgtagacg ccaggcgggc cctactactc
     1801 cggcggccag gccaaattat ctttaccgag attcacgtcg aggaatccct cggcgggcaa
     1861 gacgtccacg ggcaatcctc cgtgccacag cgccacgccg agacgcagct tactctggcc
     1921 tttgaagttg atctggtcct tgtggatggc cacttccaaa acgcgctcga aggccgcttc
     1981 cgcgacaacc ttggggttca gcaggcacac gcggttcttt tccacggcga acccttggac
     2041 gtgaccgcgc tccagattca cgacgagggt cacttcttcc gcgccgccga tggtgatgcg
     2101 aaactccggg tcctccagct ccgcgagcgc ctcggcgaat ggatcgatac gcacgcagaa
     2161 gcgcccttcc tcgaatccgt agcgcagttc gtggaggtag aagacgcggc cgtgcatgga
     2221 accgccgcgg cgctccggtg aatacaatcc cgccccgagc cattcgaaat aggacgtatc
     2281 cctgccatcg atcttgatat tcaaccaccc ggtaggcgcc aactgcaccg cgtgctccgg
     2341 ccggcgcttg atgggcttgg ccaattcttc cggcgctagc tgtcccagcg ccaggtagat
     2401 ggccgtcagg tgctttcgat aaagcgcatc gaactccgcg tcgttcgcgg tgctgtgctc
     2461 cgggccgaac caccagcacc agtcgctgcc ctccgcaaca agcagcgatt cgcgggcttc
     2521 cttgagggcg gtttcggtgg gggcatcgga ccttccctgt ttacgcgctt cgaatgcgcg
     2581 agcgtaggct tcgcgcgcgt cccagagcaa ttcccacgcc gcgacatctt cggcgtggcc
     2641 gatccagacg tcgaaatttg cgttgatcca cgaagcgggg aaaattccag tggtggttgg
     2701 aatttcaccg gcggcggcga tggcttcgct ggcagtgagc gcgcggaaat cttgatcgcc
     2761 ctggatgcgt ccgtaaaact cacggaggaa cgcgcgtccg ttccccgggt agtattccca
     2821 ggcgttttcg ccgtcgagga aaagacaaat cgtgagcggt tgatcacctt gcacgttttc
     2881 gccgagatgg cgcatgcgag agtggagatc cgccgcagcc tctttggcgt ccatgcggct
     2941 gtaaacgaag ccgacaagat cggacaagtg gtgatcgcga aacaatcccg cgatactttt
     3001 tcctcccaat tgcacgcgcc agggtttgta gaggcgatcg gcgttggcgg gaatgccgtt
     3061 gccgtcgcgg aagaatccga cgttcagggt gcggccgagg actccctcgt ccgttccgaa
     3121 ccattgaaat ccttcttcca cggcaatggc cagcgcttga tccgaaaccg agccttcgga
     3181 aggccacagc cccgcgggct tcacgccaaa aactctttcg tgatattcgc gcgcaaggtg
     3241 caattgttcg cgcgcatctt ccgggcgccg gtaagcgcgg cgcgggagag gcgtccccgg
     3301 attagccacg cgcgcaatat cagaatcgca gatcagggga aggatggggt gatagaacgg
     3361 agtcgtgctg atctcgatct ggccgcgctg agccgcgtca cgatacgcgg ggagcaccag
     3421 gcccaggaaa tcgagctgct ttttgcgcag ggcggacttg tctttttcgg tgtaatcctt
     3481 ccctttcgtg gccaggcggc tgacgacatc atccgttgcg aaccaggact cttccatcca
     3541 tgccaactgc gagaggagtt gcaggtcgcg ccaatcgcgc ggggtgaagg agaccagcgc
     3601 ctgcgcgccg ccagcgggac gcgaccattc gaataactct acgaagcgcg gccagcgcag
     3661 catcagctgc tcgtgattca cctgaaacgc gcgcgcgatg atttccgctt tgtcctcgcg
     3721 ggtcaactcg tcggcattct tgaatgccag agaaaaccag ggctcgatga aactgcagct
     3781 ggcgtactcc tcgagttgca cgccgagcga aggcacgacg ttgaaggtgg catggaagtt
     3841 gggaaattcg cggagcactt cgaccatgcc ccagtaatcc ttcagggcat ggaggcgcgt
     3901 ccacgggagg acgtagcgcc tgctctccgg atcgcggtac tgcggctggt gcatgtgcca
     3961 caacacaacc agatgaaccc gcttcatcgt ttgcgtctct cccatgctag tccggcggcc
     4021 ggatggtgtc cgcctgaccg ctgaaggtcg aagcggcggt tcgggctaga gctcgatcga
     4081 atcgagcgag ccaatcctgc cttaagcaag aaccgctgtg agaaaaacta tcacgtaatt
     4141 gatggtaagc gaaatgagcc gagcaagcgg tgacggcctt ttgcagggcc gctttgtttt
     4201 tttgtaccga gtccaggtgg caggaggaat tcagagttat tgaacgggga cagaattttg
     4261 cgccaacggg tccaacaagc tcgctgggag cgggcatcgc cgtcaccggc aaacggcgtc
     4321 cgctccctcc cgctctcaaa acacggcttt tcctgggcct agcgcaagtg gattctattc
     4381 gtagcgcaac gctacgagcg ggtccacgct tgctgctcga cgcgcgggaa gccagcacgc
     4441 cacgatcccg gtgatcaaca gaaccgcggg cacgcacaca agcgtccacg gatcgtatgc
     4501 tgacactccc cacaattgcg tggcaatcac tcgccctatc gccaggctga cgatcagccc
     4561 gatgcccacg cccaagccca ccagccgcaa tcccatgcga attaccattc gctgcacatc
     4621 cgaccctttc gcccccaacg ccattcgaat cccaatctct tgagttctcc gcaccgtcgt
     4681 gtacgccagc acgctataaa cgccgatggt aaccagcacg agcccgatcg cgccaaaaat
     4741 ggtcatcaga aaaaatccaa atcgcggccc cgcaaacgaa aactggctga tgtagccctc
     4801 gagtgtcccc gtaagcgtca ctgctacgtt ggcatccgtg gcccagattt catgttgcac
     4861 cgcgttcatc atcgtcagcg gttctctcgc cgtgcgcacc agaatcccgc ggaacgccga
     4921 acccgtcacc gtatatggca cccagatctc cggcagcact ggatcttgca aaccctggtt
     4981 ctttgcgtcc gccaccagcc caatgatttc aaacatgggc tctttcaccg cgtcatcaaa
     5041 ctccgcaagc cgtgcgattc tcacttgcct cccgatcgga ttctcgttcc ccagatactt
     5101 tttcacaaac gtctggttca cgatcgccag cttgcgcgct ccgttcactt ccgcttccgt
     5161 gaaccccctt ccatccctga actggatttt cagcaccgga aaatatccct cgctgaccaa
     5221 ctgaaacatc gcattccatt tttccgcgtg cgtcttgccc ggaatctcga tgtcgctcgg
     5281 aatccctcca tacggcggca gggtgctggt ttccgtcgca tccaccacgc ccggcagcgc
     5341 tttcagccgc tgcagcagcg gccggtagaa tcccgccacc tggtccgccg tcttgtaacg
     5401 ttccaccggc aacggaagcc tcgccaccaa cacatgatcc gggtgcaacc cgagcttcac
     5461 atcccgaagc gctacaaaac ttctcatcaa cagtcccgcc gccaccagca gcgtcaacga
     5521 tagcccaact tccagcacca ccagtgcatc acgcagtcgt ccgtgacgga atccgccgct
     5581 aatccctttt ccgctgtccc gcagcggctc gttcagatcc tttcgcgccg ctttcagcgc
     5641 cggcaccagc ccaaacacca gcgccgtcag cactgcgaca cccagcgtga acgctagcac
     5701 cggcgtattc agccggatca ccgcttccgc cggaataata ttctgcggca tcagcgccac
     5761 cagcgacttc agccctcccc acgccaggag cgtccccacc gccgccccgc ctgtagccag
     5821 gatcaggctc t
//
LOCUS       DASYVO010000226        23194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_175579, whole genome shotgun sequence.
ACCESSION   DASYVO010000226 DASYVO010000000
VERSION     DASYVO010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 23194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23194
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175579"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            453..1619
                     /gene="bshA"
                     /locus_tag="VGR03_07425"
     CDS             453..1619
                     /gene="bshA"
                     /locus_tag="VGR03_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HEV1994143.1"
                     /translation="MTSTMRIAITCYPTYGGSGVVATELGMELAARGHDIHFISYAPP
                     IRMDLANTRIHFHEVEVASYPLFDHPPYTLALATKMLEVFEAEALDLLHVHYAIPHSV
                     SALLARSMAAPRRLPFITTLHGTDITLVGANRSYLPITRFSIEQSDGVTAISDYLRQR
                     TLQEFDIKHPIEVIPNFVNCDLYQRSAGTALRAKWSPNGEPILMHLSNFRPVKRVNDV
                     VEIFAKVREKMPAKLVMIGDGPDRGSAEYLVRKKRLQKDVCFLGKQDQVYESLAAADL
                     FLLPSQLESFGLAALEAMACEVPVIATNVGGLPEVVENGVDGYLVEPGDVAEAARRAI
                     EILSRADRGREMGQTARVNARKKYCANDVIPVYERYYQRVLEQSSAASVARAHP"
     gene            1927..4596
                     /locus_tag="VGR03_07430"
     CDS             1927..4596
                     /locus_tag="VGR03_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1994144.1"
                     /translation="MAQPSSKRTNGTAVADLTGTTVGRYAIRSRVGSGGMGEVYRADD
                     TSLKRSVALKRIAPRLQSDPSYRQRFLREAQFASGLNDQHIARVYDVLEANDEMFVVM
                     EFVDGVSLRQRLAEPMGIEPFLKIGVQCAEALVAAHGHGVVHHDIKPENIMLTPTGQV
                     RVLDFGVAKQLFRAEDLVTLETVDVESASSGGGTLPYMAPEVLLGKESDSRADIYSLG
                     VVFYEALAGHHPFRAGSFVSTTSRILHELPAPLRQRNPKASAELERIVTKMLTKDPEG
                     RYATAADLLVDLRAEQRALAYPAPPGATPAGVPPDVSKERAAAPRWKMPRRRLIAAAG
                     ALLAVGIAWLPIVRRNIHWLAPVESGSKNLAVLPFQAIGGGPENQVYCDGITETLTVK
                     LSQLTASHNLQVTSAADVRSRRVSNAAQARDELGADLALEGTLLRAGNTIRVNYALVN
                     TGTLKQLRAETITADASDPFALQDKVAAGIVRMLELELKPQERKVLESHGTQVAGAYD
                     FYLQGRGYLQGYDKPENIDNAISVFQRALSLDPNYALAHAGLGEAYWQKYQAGKESSW
                     IDASRRACDRAVAIDATLAPAHVCLGTLHNGTGKYADAVEDFQQALKAEPTSDAAYRG
                     LGSAYEHLGKLQEAERTYREAISLRPNYWASYNWLGAFYYRQARYAEAAEMFGQVVAL
                     APDSFRGLNNLGAMDIVAGRYDAAVTTLQRSLALRPTAAAYSNLGNAYFYQRRFADAA
                     RAFEEALKLDERNYTVWWSLGDAYQWMPEKRALATGPYQKAITLASKKLEVNPRDTDA
                     LQVRAFCRVMLKEKKPALADLAAALKIAPNDADLNFQAALVYNQLGNAEEALKSLERA
                     FANGYAPSRVRDTPYFENLHSNPKFQALLQARR"
     gene            4614..4955
                     /locus_tag="VGR03_07435"
     CDS             4614..4955
                     /locus_tag="VGR03_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994145.1"
                     /translation="MTDSGAVPAKGTPQQKDKQVTITVAANQKDVIVAPDPVRLNKFA
                     KHTATWVCTQGLPFEVEFEETPFQGSTFNHDTAKNLIPRDDVINNAQHTYKYSVSVAG
                     ITKDPGLIVDP"
     gene            5207..5548
                     /locus_tag="VGR03_07440"
     CDS             5207..5548
                     /locus_tag="VGR03_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994146.1"
                     /translation="MTDYVAEKTKKKTQAKDFVVTIIVTQNPAIVIVVPDLVRINKFE
                     KDTVSWVCPQGLDFKVDFPETPFHDHHFDQRTAKNLLPREDVKNNANRPYKYNIIVAG
                     ITEDPGLIVDP"
     gene            complement(5644..7044)
                     /locus_tag="VGR03_07445"
     CDS             complement(5644..7044)
                     /locus_tag="VGR03_07445"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEV1994147.1"
                     /translation="MRIADLWRWEGKVGRETYALVGLIGFAIKNNLDRYVAVGFRRPW
                     PLWSYWAPLAPTMRITSLTRADAVFVGTMLALSLPFIWLGVTMTVKRLRDAGQPVWLV
                     GLFFVPVVNLLLFALLSVMPARVDEGKAEAAPWPGPRALDRVIPRSGVGSAALAIVVT
                     MVIGLSLALLGTVVVKGYGWGLFVALPFCLGLFSVLLYGYHQPRGFASCMGVAVLSVG
                     LLGLTLLTFAVEGLICLIMAAPIALCLVLMGGALGYAVQRRHWSRQAAPAMLSVVLLF
                     VPAFFGIERAANLEPPTLEVRSAIEVNAPPEIVWKQVVAFSEIPPPKELLFRAGIAYP
                     IRAEMIGRGVGAERHCIFSTGAFVEPIEVWDAPRLLKFGVTANPAPMQEWTPYAHIQP
                     PHLHGFLVSRAGQFQLTPLPGGRTRLEGTTWYRHTMWPAAYWQVWSDGIIHRIHLRVL
                     EHIRTLAEKDAVANAT"
     gene            complement(7063..7461)
                     /locus_tag="VGR03_07450"
     CDS             complement(7063..7461)
                     /locus_tag="VGR03_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994148.1"
                     /translation="MRTVLFFLWIAGAVQLVICAANFLLPRKLNYRENLSRVSPIVRQ
                     VFLVHSAYIVGVVALFAALTFSFAAELASGHGLGRFLAAAIALFWLCRVPVQMFYYDA
                     ELRRSNRAGDIVYSAAAVFLAATYGWAAFA"
     gene            complement(7479..7898)
                     /locus_tag="VGR03_07455"
     CDS             complement(7479..7898)
                     /locus_tag="VGR03_07455"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEV1994149.1"
                     /translation="MISLANEFTDGKGRHARGWLFFDAECAFCTRIARWLVPILERRG
                     LAIAPLQDPRVGALLGLSREELLREMRFVLADGSRTGGADAAVALAHEIWWWRPLVWL
                     ARVPGAMGLMRMVYQRVARSRQCAATGCEADAASRRV"
     gene            complement(7944..8507)
                     /locus_tag="VGR03_07460"
     CDS             complement(7944..8507)
                     /locus_tag="VGR03_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HEV1994150.1"
                     /translation="MSDTTNNPLTPVEQKFILHWGEMGTRWGINRTVAQVHALLFLSQ
                     RPVPADEIAKTLAVARSNVSTSLRELQGWGIVRVVHVLGDRRDHFESVKDVWEIFRIV
                     AEERKRREIDPTLRVIRECVEGLKADRKSDAYTRERLESMLEFLTAMTGLFEEILRMP
                     TGALKGVVKLRGKVITLLGAGKAKIAG"
     gene            complement(8685..10004)
                     /locus_tag="VGR03_07465"
     CDS             complement(8685..10004)
                     /locus_tag="VGR03_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV1994151.1"
                     /translation="MDTLPSVKELLFTLLLKVGVASSFAALLARWTVLRKVLYTEERT
                     PDQKLKLMFFMAPPLLVGVTLRLVGQPYRFADLSLEGAFLLGLLGGRVVGPIGGAIIT
                     IPALIAREWLAMPVASAAGLIGGLIRQAIPDKEDIWNFGPFTFLNIPRAIVRLVRHAQ
                     ISWEMLPLFTCVGMEIGRVALVRATKPRWLFAIDAHRDWGLALDVLATLMAVAAPLKI
                     WNNTRVEMNLERHRELLLKARMDALSSQINPHFLFNTLNTVSALIRYDPDTARGVVLK
                     LSNILRRLLRKHETFVPLRDELEFVDDYLDIEVARFGRDSLEIVKHIGEETLEAFVPS
                     MLIQPIVENCLKHGLAPKLEGGKIQLRTSSRGGRLQIEIEDNGTGISEEKMPNVYVEG
                     IGLSNVRERLRVLYGADFQLEIQSQPGEGTLIRIEIPELVSAQPVPG"
     gene            complement(10036..11442)
                     /locus_tag="VGR03_07470"
     CDS             complement(10036..11442)
                     /locus_tag="VGR03_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994152.1"
                     /translation="MRIAFSRRRSFLTTSLFLSFAFFPSPPAWTQQVTPTPGKPAPIA
                     GEKEGSLAAADEVLEQVSALTKLSLRTPVKKSLRSREEIRAYVLKRMREDKSADERYA
                     AERAAVAFGLVPKDFQMEAFLVELLTEQIAGLYDPQGHEFYIADWIPLEDQRMVMAHE
                     LTHALEDQHFHLEKWLKAARPNDDAELAREAVLEGSATAAMIDYLLQDSGRSLKDLPD
                     VDPELLVGDLGDTPTLKKAPPFIKDALIFPYFSGLRFSKAFLEKSGWSGLASLFEHPP
                     VSTQQILHPELYREGRRPVHGDLSVNPKLLRGAWKQLLDNTLGEFGWKEVLQQGLGKE
                     RAVALSSAWSGDRYVLLEEKSSKKLVLVYRVFWSDEEHAARFFGQYSEALEKKYAARS
                     NLLRKPDFFSFETADGTVALRCVRADCVVVEGAPRVFINDVYRRIGWSPAPEPPPARE
                     KEIEKTASAVLYFAAGIV"
     gene            11720..12523
                     /locus_tag="VGR03_07475"
     CDS             11720..12523
                     /locus_tag="VGR03_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994153.1"
                     /translation="MMVPGLLLRQTRERLGLTYRDVERASFELASRRGRPEFIIRISR
                     LADIENHAAVPGLHKLYSLAAIYHLNPLEVCHWYEVPLEEFFHDGAYLHAPQTHLGAA
                     PATLRVPLRFDPAFNPQRTEFLSRMVERWGRFEGALTNGDLHTLYGFIGLSDHRMEPL
                     LRPGSIVLIDTALRRIEKGSWRSEFERPMYFVDVRVGYRCGWFDLTGDRLSMHPHPLS
                     RCAPEYWRYPEDVDVIGRVAGIVTRLYETAGCLAAECPEEPGDSSRKAS"
     gene            complement(12441..12890)
                     /locus_tag="VGR03_07480"
     CDS             complement(12441..12890)
                     /locus_tag="VGR03_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994154.1"
                     /translation="MGILTQLQQNRRIIHDFTVTSLSGIPGIFARLTYLASLRDLSSG
                     RYEHAGLEAIYPAAAVQQALEQCHEELFERLLEMPLKAQQEDLRACLSGMQGGVTSAV
                     EHWQSLESYRILIPEAAPDYLKTLFCSNLRALLGILQQDSRQSHTTA"
     gene            13358..14134
                     /locus_tag="VGR03_07485"
     CDS             13358..14134
                     /locus_tag="VGR03_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994155.1"
                     /translation="MAFTQYAAWAACQAVLVFLLAGLLRSGNHRQFPVFTAYILSNLG
                     QAALLVFSYRQWGPQAPVTQQIFWITTGVTLLARALAIAELIHRILGLHKGIWGLAWR
                     SIAVSAVVIAAYSFLVANRRWDLGLIAADRALELALGCGIVVLFVFCRYYEVQVPRAE
                     RIVGMGLILYSGVVVISHTFLERFFYAYSPFWNLTILTSFFVSATLWIRALWVPVPSM
                     RAQPAMLGQLYQTLSPEINLRLRALDQRLISLWKREAPNS"
     gene            14131..14709
                     /locus_tag="VGR03_07490"
     CDS             14131..14709
                     /locus_tag="VGR03_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994156.1"
                     /translation="MTWALLTFFFLAGGLLVLFAWSLYQPRKHFPSTLPAAVNDDSPR
                     RHAPHLAQISQSLSPEDIAFLMTRGSRVIARRAAAERRHTAIRYVEVLHEDFRSLFQM
                     GRVLASLSPEIKGREEAERLWLAARFECRYQIVRAMLLLRGSPVLQLQQMTQIVGRLS
                     RRLDAEMAEWGERTALAMEMGSVADRRHPDTF"
     gene            complement(14674..18246)
                     /locus_tag="VGR03_07495"
     CDS             complement(14674..18246)
                     /locus_tag="VGR03_07495"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV1994157.1"
                     /translation="MAEPTLPGHDDPERMRVIVWQNQGLLGIAFFEATTSFIFLVLFF
                     YLQRDYRLAYLRTWLAGWVVLTVTGFAGLASLVYSGGLPFLRIITVTGEIAAFLLFLA
                     GVTQLISGSAPARWPLIPLACLAILGDLYLEAKVPAQFGKLRWETAALESFLAIAAGW
                     VLWRCTVATRGVSAKFLAGAFFLIGLHGLDRPDWPEHPLYLVRLAFGDLLFVVLGMGM
                     IVFVLEGARARAEELNDKLRRLTLLATASTQTFSVKEVLDKVLVSLVGSLAATHGAVR
                     LLEGSEGSAELVLKSSVGFTPAFNEKYDRLPVREPWVQRVLSVGLVAHTLDDETDERT
                     RNRMIEGGITQLVTVPLPGKNGPQGLLVLGSSHPVKFPQDEISFLGNVANLLGLTLLN
                     VHLFEQAATAQKQWAYTFDSIGDPVLVHDPEYRIVRNNQRFSQILGRHPSAMYGRSIG
                     EIFPRRAGNYDQCPYCEGSAGGGDDPDPWLGGYFLASNSTFTDPQGRQLGTVHVLKDI
                     TDRKRAEEKYRSLISNVQEGVFISTPQGRFTDFNDALMRMAGYESREELMGANIPSLY
                     ANPADRERLKKLLHEHGSVSDFEFEWLRKDGEVRTVLESSIGIRDAAGEVTAYQGFLL
                     DITERKRAEQEIRRRNRELMVLNSIGQTLNESLDLKDSVRRTLRQLVELFQLDASSLY
                     LLDEKQTMLQCVAAVGQQSGHSRVHSSIPVQPDLLQHVRAVRATFLSTLGLPLPPAFR
                     EAQSEDGIVTAYVVVLWSKDRVIGGLFVGSRNPREFSPADVNLLIAVGVQISSAIERS
                     LLFEETRQAYENLRRTQEQLLHSEKMAAIGQLISGVAHELNNPLTAILGYSQLLSANG
                     QIGPQAVEYSEKLYKQAQRTHRIVQNLLSFARQHKPERVPVAINQVIVDTLALRDYDL
                     RLSNIRLNLDLASDLPVTEADPHQLQQVFLNLVNNAVDAILEHSREGQIWVRTASEED
                     HLIIEFADSGPGVQDPARVFDPFYTTKPVGKGTGLGLSICYGIVMEHGGTIQVRNAPP
                     RGATFTIAFPWQHKAEIEQPAGGDVAPAGKRRRILLIDDDDSVLEAVSETLRARKHFV
                     QTAQTADRAKEYLMAEDFDLILADTHLPGRREGENLLHWLAEAKPDLVGRVVLMSSGT
                     GPAEAVRPQPFSSSRVLQKPFKTAELIATLEETLKRVRVPSISD"
     gene            18377..19456
                     /locus_tag="VGR03_07500"
     CDS             18377..19456
                     /locus_tag="VGR03_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEV1994158.1"
                     /translation="MEPSKILIVDDEESIREVVATMLRAQGFHCVAVCNGREAQDQVL
                     KMPPDLVLSDMIMPEMDGVRLLEWLRQYDPDIPVIMVTAIHDISTALEAIRRGAYDYI
                     LKPFEKDQLYHAVGRAIQHRTLVLENHSYQRNLEVLVEERTAQLSGTLAQLEQSYDDT
                     LEALGSALDLRDAETEGHCQRVTAFTVYIAKTMNVPPEDLPILARAAFLHDIGKMAIP
                     DVILRKPGPLDDDEKRIMRTHCEIGYNMLIRIPFLRTAAEIVLAHQEFFDGAGYPRGL
                     RGEAIPLGARIFAVADALDAMLSDRPYRRALPFDHALAEIQRCSGSQFDPKVVEVFLS
                     IPHSHWTEMRQNMDSPFRLAHLQNL"
     gene            19537..19794
                     /locus_tag="VGR03_07505"
     CDS             19537..19794
                     /locus_tag="VGR03_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994159.1"
                     /translation="MALVPIKTASETGYCDCVCGCYELASLRDEDTGKGFCPNCGKQH
                     YELNAGVGIPQPAPARVSANSSGTALDTPVPTAADPPGRLR"
     gene            19940..20515
                     /locus_tag="VGR03_07510"
     CDS             19940..20515
                     /locus_tag="VGR03_07510"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:20177..20179,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEV1994160.1"
                     /translation="MNLKSLTGTILAGALCLSAYGLTAAQQKQEKIAHTNLKVGDVAP
                     DFTLLDNKWKPVHLSDYRAKKNVVLAFYVLAFTGGUTKELQAYQADKAKLEASDTVVF
                     GISIDSPAANDAFAEKIGVTFPLLSDMNRKVLKQYGILKSYNVQSDDYEWAQRTTFVI
                     DKAGVIQHVEEGNGAVNPNNAIAVCTSLPKK"
     gene            20552..21703
                     /locus_tag="VGR03_07515"
     CDS             20552..21703
                     /locus_tag="VGR03_07515"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEV1994161.1"
                     /translation="MTTRPVPSKAGPATEPIPQFDLHAQYAAIQGDIRAAIEDVFSSQ
                     QFILGRQGEALEQEIAGVCGVAHGVGAASGTDALILALRVCGVGAGDEVLLPPFTFVA
                     TGSAVSALRARPVFADIDAATFNLDPHELERRITPRTRAIVAVHLYGLPADMDPILAF
                     AREHSLPVIEDAAQAIGASYKGRPVGSLGDAGCISFYPTKNLGAYGDAGMLVTNSAEI
                     AKRARRLRNHGQTGKYVSDEPGLNSRLDELQAAILRVKLKHLPEWQRARQQHAAEYSR
                     LLEGIAGLTTPRVPAGCEHVYHQYTIRLPERDRVLKTLAEQKIGSRVYYPVPLHLQPL
                     YASLGYKPGDFPHSERAAQEVLSLPMYPELRDEQIRRVAAALAEAISPS"
     gene            complement(22107..22433)
                     /locus_tag="VGR03_07520"
     CDS             complement(22107..22433)
                     /locus_tag="VGR03_07520"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEV1994162.1"
                     /translation="MSAHDNAETAGHVPAKSSLFVIVWFGLVALTGIEVFLGYNQIAP
                     GIMLTVLIGLSIVKAGLIMAYFMHLKYEKFGLVLLLVPAFMFCVMMICVFFFPDSLRL
                     GNPVMH"
     gene            complement(22443..23072)
                     /locus_tag="VGR03_07525"
     CDS             complement(22443..23072)
                     /locus_tag="VGR03_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEV1994163.1"
                     /translation="MDHAHEEAPVSSAWSGGTRPFAMGSKKLGMWLFIISDSLTFSAL
                     LLSYSYLRLANHDWPRPFPFSPSIVFSTVMTVVLLASSLTMVFGVAAAARGDRGTAVK
                     WILATMFFGAAFIGLHLREWLHLIEEGLRPFGNPWGSPVFGAVFFGVTGLHMTHVAIG
                     VIYLGIVALGFGRSKFSAEDVEVSGLYWHFVDLVWMFVFPLVYLMSIKY"
BASE COUNT         4749 a         7347 c         6629 g         4469 t
ORIGIN      
        1 tcccctccag ttcgcccgca accctttgga attcgcactt ccgaaaaccc actcccgcaa
       61 ccccttcaga atcaacactt gcaaaagtgt atcaaaacaa agaactttaa ccccctttgg
      121 aatcaacact tacaaaaaac aggggggagg gggagtatct cttctgccaa cagtcctggt
      181 actccgcttc cgagccgatc accactcacc ggccatcaat cacccttcct tcacttcctc
      241 tacctcatct atctccttta cttccttcct cttccgcact ctttctcact cttctcccgc
      301 aagtcctttg tttgccactc atacgcaaaa caacccgggg gtggggggtc tctgcgagtt
      361 ttttctttac ctcgtcacct cattgcctct ttgcttccca tcctcgctat gtcactcttt
      421 ctctgccctt tgcgtctaat gggagcggct cagtgacctc caccatgcgc atcgccatca
      481 cctgctaccc cacctacgga ggctccggcg tagtcgccac ggagcttggc atggagcttg
      541 ccgcacgcgg ccacgacatc catttcatca gctacgcccc gcccatccgc atggacctcg
      601 ccaacacacg cattcacttc cacgaagtcg aggtcgcctc ctacccgctc ttcgaccatc
      661 ccccctacac cctggccctc gccaccaaga tgctcgaagt ctttgaagcc gaagccctcg
      721 acctgctcca cgtccactac gccattccac attccgtcag cgcgcttctt gcccgctcca
      781 tggccgcgcc gcgccgcctg cctttcatca ccacgctcca cggcacggac atcaccctgg
      841 tcggcgccaa ccgcagctat ctccccatca cgcgcttttc catcgagcag agcgacggcg
      901 tcaccgccat ctccgactat ctccgccagc gcaccctcca ggagttcgac atcaagcacc
      961 ccatcgaggt catccccaac ttcgtcaact gcgacctcta ccagcgctcc gccggcacgg
     1021 ccctccgcgc gaagtggtcc cccaacggcg aacccatcct catgcacctc tcgaacttcc
     1081 gccccgtcaa gcgcgtcaac gacgtcgtcg agattttcgc caaggtccgc gagaaaatgc
     1141 ccgcgaagct cgtcatgatc ggcgatggcc ccgaccgcgg ctcggccgag tatctcgtcc
     1201 gcaaaaagcg cctgcaaaag gacgtctgct tcctgggaaa acaggatcag gtttatgaga
     1261 gtctcgccgc ggccgatctc ttcctcttgc cctcgcagct agagtccttc ggcctggccg
     1321 cgctcgaagc catggcctgc gaagtccccg taatcgccac gaacgtcggc ggcctccccg
     1381 aagtcgtcga gaatggtgtg gacggttatc tggtcgagcc gggtgacgtc gccgaggccg
     1441 cgcgccgcgc cattgaaatt ctttcccgcg ccgaccgcgg ccgcgaaatg ggccagaccg
     1501 cccgcgtcaa cgcccgcaaa aaatactgcg ccaacgacgt tatccccgtc tacgaacgct
     1561 actaccagcg cgtcctcgaa cagtcctccg ccgcctccgt cgcccgcgct cacccatgac
     1621 gagcccgatc catcatctcc actccgcgcg catgtcggcc cgtccggaca gtcgtcggcc
     1681 gcctccgcct gaatcgcccg cccggttttt cggcgaagca ccggccatgc gtgaccgagc
     1741 attgacactt cccggtgcct gccgtatggt ctcttacata aagtgttcgt tggcatcgta
     1801 ggacccgacg gaaaaatgcc gacatgccaa atcatcggag tgttcgtgga attggcagtg
     1861 gttgcccgca tcgccaccgt tgatttgctt taagccctga tcttgcgtcc gggccctcag
     1921 tatccaatgg ctcagccttc atccaagcgg acgaacggaa cggccgtcgc cgatctgacg
     1981 ggcacgacgg tgggccggta tgccatccgc agccgggtgg gctccggcgg gatgggcgag
     2041 gtgtaccgcg ccgatgatac aagcctgaaa cgatctgtgg cgctgaagcg catcgcgcca
     2101 cggctgcaat ccgacccgag ctaccgacaa cgcttcctgc gcgaggccca gttcgcttcc
     2161 ggcctgaacg accagcacat tgctcgtgtt tatgatgtcc tcgaagccaa cgacgaaatg
     2221 tttgtggtca tggagttcgt cgacggcgtg agcctgcgcc agcggctcgc cgaacccatg
     2281 ggcatcgagc cgttcctgaa gatcggagtg cagtgcgccg aggccctggt ggctgcgcat
     2341 gggcacggcg tcgtacacca cgacatcaaa ccggaaaaca tcatgttgac gcccacgggg
     2401 caggtaagag ttctcgactt cggagtcgcc aagcaactct tccgcgccga agacctcgtg
     2461 accctggaaa ctgtggatgt ggagagcgcg tcttccggag gaggcacgct gccgtacatg
     2521 gctccggaag tattactcgg caaggaatct gattcccgcg cggacattta ttcgctgggc
     2581 gtggttttct atgaggcact ggccggccat catccgttcc gggcgggcag cttcgtgtcc
     2641 acaacctcgc ggattctcca tgagcttccg gcaccgctgc ggcaacggaa cccgaaggcc
     2701 tccgccgagc ttgagcgcat cgtgaccaag atgctgacga aggatccgga gggacgctac
     2761 gctacggctg cggacttgct cgtggatttg cgcgcggagc aacgcgctct ggcgtaccct
     2821 gcgccaccgg gcgcgacgcc ggccggcgta ccgccggacg tttcgaaaga acgagcggcg
     2881 gctccgaggt ggaaaatgcc acgccggagg ttgatcgccg cggccggagc cttactggcc
     2941 gtaggaattg cctggctgcc catagtaagg cggaatattc attggcttgc gcccgtggag
     3001 tcgggttcca agaacctggc cgtgctgcct ttccaggcca tcggcggcgg gccagagaac
     3061 caagtctact gcgacgggat aacggaaaca ctgaccgtca agctgtcgca actcaccgcc
     3121 tctcataacc ttcaagtgac ctcggccgcg gacgtgcgtt ctcgccgcgt ctccaatgca
     3181 gcgcaagcgc gcgatgaact gggcgccgac ctggccctgg aaggtacgct gctccgcgcg
     3241 ggaaatacca ttcgcgtgaa ctacgcgctg gtgaacactg gcacactcaa acagctccgc
     3301 gcggagacga tcacggcaga cgcttccgac ccctttgcgt tgcaggataa ggttgcggca
     3361 ggcatcgtgc gcatgctgga gcttgagctg aagccccagg aacgcaaggt gctcgagtca
     3421 catggcacgc aggtggccgg cgcctatgat ttctatctgc aggggcgcgg ctacctgcag
     3481 ggctatgaca agcctgaaaa tattgacaat gcgatcagcg tcttccagcg ggcgcttagc
     3541 ctcgacccca actatgccct ggctcatgcg ggcctgggag aagcctattg gcagaagtac
     3601 caggcgggga aagaatcttc ctggatcgat gcctcccgaa gagcctgcga tcgcgccgtg
     3661 gcgatagacg cgaccctcgc gccggctcat gtttgcctgg gaacgcttca caacggaacg
     3721 gggaaatacg cggacgccgt cgaggacttt cagcaggctc tgaaagccga gccgaccagc
     3781 gacgctgctt accgcggtct cggctccgcc tatgagcatt tgggcaagtt gcaggaagcc
     3841 gagcggactt accgcgaagc gatctccctg cggccgaact actgggccag ctataactgg
     3901 cttggcgctt tttactaccg tcaggcccgc tacgcggagg cggcggaaat gttcggtcaa
     3961 gtggtggcac tggcgcccga tagcttccgt ggcctcaata atctgggggc catggacatt
     4021 gttgcggggc gctacgacgc agccgtcacg acgctccagc gctcgcttgc cctgcggccg
     4081 acggctgccg cttacagcaa cctgggtaat gcatacttct accagagacg gtttgccgat
     4141 gccgcccgcg cctttgagga agcgttgaag ctcgacgagc gaaactacac cgtgtggtgg
     4201 agcctgggcg atgcctacca gtggatgccg gaaaaacgcg cactggccac agggccctat
     4261 cagaaggcca tcacgcttgc gagcaagaag ttggaggtca atccgcgcga caccgacgcg
     4321 ctgcaagttc gggccttctg tcgcgtcatg ctgaaggaaa agaagccggc cctcgccgac
     4381 ctggccgccg ctctgaagat cgcccccaat gacgcagacc ttaatttcca ggcagcgctg
     4441 gtctacaacc agttgggcaa tgctgaggaa gctctgaagt cgctggaaag agccttcgcg
     4501 aatggatatg ctcccagccg ggtgcgtgac actccgtact ttgagaatct gcactcgaat
     4561 ccaaagtttc aggccctgct tcaggcgaga cgctagttca aggaggaaaa cacatgaccg
     4621 attccggcgc agtaccagcg aaaggcacac cgcaacaaaa ggacaaacaa gtcacaatca
     4681 ctgtggccgc aaaccagaag gatgtgatcg tcgctcccga ccccgtgcgg ctcaacaagt
     4741 ttgccaagca tacagcgacg tgggtatgca cacagggact tcctttcgag gtggagtttg
     4801 aggagacgcc gttccaaggc tccaccttca atcacgatac ggcgaagaat ctcattccgc
     4861 gcgatgacgt catcaacaat gctcaacaca cctacaagta cagcgtgagt gtggccggaa
     4921 taacgaagga cccgggcctg atcgtcgatc catgagagtg acctggtaaa ttgggggtgt
     4981 tttggcaatc ccgtcgatga ggcgtgtctt ggctgaggtg tgtttctctc gtcagagttt
     5041 ccgacgtttc ttgcgtcgcg ttcgtgaccg cgtcctcgga aagagccttt gcgaatggat
     5101 atggtccgag ccgggtgcgc gacactccct actttgagaa tttgcactcg gatccaaagt
     5161 ttcagggcca gcttcaggcg aaacgctagt tcaaggagga aaacacatga ccgattacgt
     5221 cgcagagaag acgaagaaga aaacacaggc aaaggacttt gtggtcacaa tcatcgtgac
     5281 ccaaaaccca gcgatagtga tcgtcgttcc cgatctcgtg cgaatcaata agtttgagaa
     5341 ggatacagtg agctgggtgt gcccccaggg actagacttc aaggtggact tccccgagac
     5401 accgttccat gaccatcact tcgatcagcg cacggcgaag aatctcctcc cgcgtgagga
     5461 cgtcaaaaac aatgctaacc gaccgtataa gtacaacata atagtggccg gaattaccga
     5521 ggatcctggc ctgatcgtcg atccgtgaag aagggctggt cgtttgggag tgttgtgaca
     5581 agttcagaat tgaggtgtgt ccgggctggg gtgtgtttct ctcgtcagag tgtttgaagt
     5641 ttcttacgtc gcgttcgcga ccgcgtcctt ctccgcgaga gttctgatgt gctcgagaac
     5701 acgcaggtgg atgcggtgaa tgatgccgtc cgaccaaacc tgccagtacg ccgccggcca
     5761 catcgtgtgc cggtaccacg tcgttccctc cagccgcgtc cgccctcccg gcaacggcgt
     5821 caactggaat tgtcccgcgc gtgaaaccag gaaaccgtgc agatgcggag gttggatgtg
     5881 cgcgtaaggc gtccactcct gcatcggcgc tggattcgcc gtcactccaa acttcagcag
     5941 ccgcggcgcg tcccacacct cgatgggctc cacgaacgca cccgttgaga aaatgcagtg
     6001 ccgctccgca cccaccccgc gcccgatcat ctccgcgcga atggggtacg caatccccgc
     6061 gcgaaacaac agctcctttg gcggcggaat ctcactgaat gccacgactt gtttccagac
     6121 gatttccggc ggcgcgttca cctcgatggc cgagcgcact tccagggtag gcggctccag
     6181 gttggccgcg cgctcgattc cgaaaaacgc cggcacaaac agaagcacca ccgacagcat
     6241 cgccggcgcg gcctggcggc tccaatgccg cctttggacg gcgtacccca gcgcgcctcc
     6301 catcaacaca aggcacagag cgatcggcgc ggccatgatc aggcagatga gcccctccac
     6361 cgcaaatgtc aagagagtca agccgagcaa acccaccgag agcacagcca ctcccatgca
     6421 actcgcaaat ccgcggggct ggtggtagcc gtacagcagc accgaaaaca gccccaggca
     6481 aaagggcagc gcgacgaaca gaccccagcc ataacctttc acaactaccg tccccagcaa
     6541 cgccagcgac agaccgatta ccatcgtcac cacaattgcc agcgccgcgc tcccgactcc
     6601 gctccgcgga atcacccggt cgagtgcccg cgggcccggc cacggcgcgg cctccgcttt
     6661 tccttcgtcg acgcgggcgg gcatcacgct gagcagcgcg aacaagagaa gattcaccac
     6721 cggcacaaag aacagcccca ccagccacac cggctgcccc gcatcccgca gccgcttcac
     6781 cgtcattgtc acgccgagcc agatgaacgg tagggacagg gcgagcatag tccccacaaa
     6841 caccgcgtcc gcgcgagtta gcgacgtgat ccgcatcgtc ggtgcgagcg gcgcccagta
     6901 gctccacagg ggccatggcc gtcgaaatcc aacggcaacg tagcgatcca ggttgttctt
     6961 gatcgcaaag ccgatcagac ccaccagcgc gtacgtttcc cttccgacct tcccctccca
     7021 ccgccacaaa tccgcgatgc gcatcgcagt cctcccgtgg tcttacgcga acgccgccca
     7081 gccatatgtc gcggcaagaa agaccgccgc cgccgaatac acaatatctc ccgcgcgatt
     7141 ggaacgccgc aattccgcgt cgtaataaaa catttgcacc gggacgcggc agagccagaa
     7201 cagagcgatg gccgccgcaa gaaatcgccc gaggccatgt ccgcttgcca attcggccgc
     7261 gaaagaaaat gtcagcgccg caaacaatgc cacaacgccg acaatgtaag cggaatgaac
     7321 aaggaagact tgacgaacga tcggggaaac tcgcgataga ttctcgcgat agttcagctt
     7381 gcgaggaagg aggaaattcg cggcgcagat cacgagttgt acggctcctg caatccagag
     7441 gaaaaagagg acggtgcgca tgagggcctc ccttcgtgct agactcgccg cgaagctgca
     7501 tctgcctcgc agcctgtcgc ggcgcattgc cggctccttg cgacccgctg atagaccatt
     7561 ctcatcaacc ccatcgcgcc cggcaccctt gcgagccaaa ccagcggacg ccaccaccag
     7621 atctcgtgag ccagcgccac ggccgcgtcc gcgccaccgg tgcggcttcc gtccgccagc
     7681 acgaagcgca tctcccgcag cagttcttcc cgtgacagac ccagcagcgc tcccactcgc
     7741 ggatcttgca gcggcgcaat ggccagcccg cgccgctcca ggatcggtac cagccagcgc
     7801 gcgattcgcg tgcagaaggc gcactcggcg tcgaaaaaca gccagccccg cgcgtgtctt
     7861 ccctttccgt cggtgaattc attcgccagc gaaatcatgc tcttggtggg acgggcttcc
     7921 gcccgtccta ctcctcactc tcttcatccc gcaatcttcg ccttgcccgc gcccagcaaa
     7981 gtaatcacct tcccgcgcag cttcaccaca cccttcagcg cgccggtcgg catccgcaaa
     8041 atctcttcga atagcccggt catggccgtc aggaactcca gcatgctctc cagccgctct
     8101 cgcgtgtaag cgtcgctttt ccggtccgcc ttcagccctt ccacgcactc ccggatcacg
     8161 cgcagcgtgg ggtcgatctc cctgcgcttc cgctcttcgg ccacgatccg gaaaatctcc
     8221 cagacatcct tgacggactc gaaatggtcc cggcggtctc cgagaacgtg caccacgcgc
     8281 acgatccccc agccctggag ctcgcgcagg ctcgtgctta cgtttgaccg cgccacggca
     8341 agcgtcttcg caatttcatc cgcggggacg ggccgctggg agagaaacag cagggcgtgg
     8401 acctgggcca cggtgcggtt gatgccccag cgggtgccca tctcgcccca gtggaggatg
     8461 aacttctgct cgacgggggt cagcgggtta tttgtcgtgt ctgacatttc agtctgtaca
     8521 gaaactacag tatatccagc cggctgtcaa gtcctttttt tcgttccccc ggcgggcccg
     8581 cgcgatgcgg ccgcggcgac ggccgcgccc cgccaacggc ctggccttgc atgttttcgt
     8641 gcgtggatgg tccgcggggc aagccaaact tgcggtcccc taccctaccc gggcacgggc
     8701 tgcgccgaga ccagttcggg aatctcgatg cggatcagcg tcccttcccc gggctggctc
     8761 tggatctcga gttgaaaatc cgcgccgtag agcacgcgca gccgctcgcg cacgttgctc
     8821 aagccgatgc cctcgacgta cacattcggc atcttttcct cggagatgcc tgtcccgttg
     8881 tcctcgatct cgatctgcag cctgccaccg cggctcgacg tgcgcagttg gattttcccg
     8941 ccttccagct tgggggccag cccgtgcttg aggcaattct ccacaatcgg ctggatgagc
     9001 atgctgggca cgaatgcttc cagcgtctcc tccccgatgt gcttgacgat ctccaggctg
     9061 tcgcggccga accgcgccac ctcgatgtcc aggtaatcgt caacgaattc gagttcgtcc
     9121 cgcagcggca cgaaagtctc atgctttctc agaagcctcc gcaggatgtt gctcagcttc
     9181 aggaccacgc cgcgcgccgt gtccgggtcg tagcggatca gggcggagac cgtattcagg
     9241 gtattgaaga ggaaatgggg gttgatctgg cttgaaagcg cgtccatgcg cgccttcagc
     9301 agcagttccc ggtggcgctc caggttcatc tcaacgcgcg tgttgttcca tatcttgagc
     9361 ggcgccgcca cggccatcag ggtcgcgagc acgtccagcg ccaagcccca gtcccgatgc
     9421 gcatcaatcg caaacagcca cctcggcttc gtcgcacgca ccagcgccac gcgcccgatc
     9481 tccatcccga cgcaagtgaa cagaggcaac atttcccatg agatttgcgc gtgccgtacc
     9541 agccgcacga tggcccgcgg aatattcagg aaggtgaacg gcccgaagtt ccaaatgtct
     9601 tccttgtcgg gaatcgcctg gcggatcaat cctccgatca atcccgcggc ggacgccacc
     9661 ggcatggcca gccactcacg cgcgatgagc gccggaatgg tgatgatcgc gccgccgatg
     9721 ggaccgacca cccgcccgcc caacaggccg aggagaaatg ctccttccag ggaaaggtcc
     9781 gcgaaccggt acggctgccc gacgaggcgc agcgtcacgc cgaccagcag cgggggcgcc
     9841 atgaaaaaca tcagcttcaa cttctggtcc ggggtgcgct cttcggtgta caagaccttg
     9901 cgcaggacgg tccagcgagc cagcaacgcc gcgaacgacg acgccacgcc cactttcagc
     9961 agcagcgtaa acagcagctc tttaacagag ggcagcgtat ccataggctc ttacggcgac
    10021 ccgctcccgg taacgctaga caatccctgc cgcgaagtaa agcacagcgg acgctgtttt
    10081 ctcgatttct ttttcccgcg ccggtggcgg ctccggggcg gggctccagc cgattcgcct
    10141 gtacacgtcg ttgataaaca cgcgcggggc gccttcgaca actacgcaat cggccctgac
    10201 gcaccgcagg gccacggtcc cgtccgctgt ttcgaaggag aagaaatcag gtttgcgcaa
    10261 caaattgctc cgcgccgcat attttttctc caaggcttcg gagtattggc cgaagaagcg
    10321 cgccgcgtgc tcttcgtcgc tccagaaaac gcggtaaacc aagaccagtt tcttcgagct
    10381 tttctcctcg agcaacacgt aacgatcgcc gctccacgcg ctcgagagcg cgacggcacg
    10441 ctcctttccc aacccttgct gaaggacttc tttccagcca aattccccaa gggtattgtc
    10501 caagagctgc ttccatgctc cccgcaacag ttttggattg actgaaaggt cgccgtggac
    10561 gggccggcgc ccctcccgat aaagttcggg atgcaggatc tgctgggtgg aaaccggggg
    10621 gtgttcgaat aggctcgcca gaccgctcca tcccgatttt tccaggaatg ctttcgaaaa
    10681 ccgcagcccg ctgaagtagg gaaaaatcag cgcgtcttta atgaacggcg gcgccttttt
    10741 gagggtcggc gtgtcgccga ggtctcccac aagcagctcc ggatcgacgt ccggaagatc
    10801 cttcaggctt cggccggaat cctgcagcag gtaatcgatc atcgcggcag tggcactacc
    10861 ttccaatacg gcctcgcgcg cgagttccgc atcatcgttt ggccgcgccg ccttcagcca
    10921 tttttcaagg tgaaagtgct ggtcctcgag agcatgcgtc agctcatgcg ccatcaccat
    10981 gcgttgatct tcgagcggga tccagtccgc aatgtagaac tcgtgccctt gcggatcgta
    11041 gagccccgca atctgctccg tgagaagctc gacgagaaac gcctccatct ggaagtcctt
    11101 cggcaccagc ccaaaagcca ccgcggcgcg ctcggcggcg taacgctcgt ccgctgattt
    11161 gtcctcgcgc atccgcttca gcacataggc tcggatctct tcccgacttc gcagcgattt
    11221 ctttacggga gtccgcaggg agagtttcgt gagagcgctg acttgctcga gcacctcgtc
    11281 ggccgccgcg agcgagccct ccttctcacc ggcgatcggt gccggcttcc cgggagtagg
    11341 ggttacttgt tgcgtccagg caggagggga tgggaaaaac gcaaaggaga gaaaaaggct
    11401 ggtggttagg aagctccgac gccgggaaaa tgcaatgcgc ataagaagtt caggatacca
    11461 gaatcgagat gctcgccgag attgcccgcg ggctcatctg tttggaagtt cgtgacagcg
    11521 gatcacgctc agcggatccc ctgaaattgc ggcctctcct ggatgcttcg gcatccgaca
    11581 gagagctgcc ggtgtccctt cttggcgttc cccgtggcgc caaaacacga tttcaccgcc
    11641 cccttgttcc gtttccttaa caaactggta attctggtat atccttctta gaaatatact
    11701 ggcatcaaat ccgaggagaa tgatggtccc tgggttactt cttcggcaaa ctcgtgagcg
    11761 cctcggactg acctaccgcg acgtggaacg ggccagtttc gagctggcct ctcgccgcgg
    11821 caggccggaa ttcatcatcc gcattagccg cctggccgac atcgagaatc acgctgccgt
    11881 tcccggcctc cacaagcttt actcactggc ggccatctac cacctgaatc ctctggaagt
    11941 gtgccattgg tatgaagttc ccctcgaaga attctttcac gacggcgcct accttcacgc
    12001 gccacagacc catctgggtg ccgcgccagc aacgctgcgc gttcccctcc gcttcgatcc
    12061 cgctttcaac ccacagcgca cggagtttct ttcccggatg gtcgagcgct gggggcgctt
    12121 tgaaggggcc ctcacgaacg gtgacctcca cactttgtac ggcttcatcg gtctatccga
    12181 ccaccgcatg gagcctctgc ttcgaccggg cagcatcgtg ctgattgaca cagcccttcg
    12241 gcgcatcgaa aagggatcgt ggcggtcgga attcgaacgg ccgatgtact tcgtggatgt
    12301 gcgtgttggc taccgctgcg gctggtttga cctgaccgga gatcgcctga gcatgcatcc
    12361 ccatccgctc tcccgatgcg ctccggagta ttggcgctac ccggaggacg tggatgtgat
    12421 cgggcgcgta gccggaatcg ttacgcggtt gtatgagact gccggctgtc ttgctgcaga
    12481 atgcccagaa gagcccggag attcgagcag aaaagcgtct tgaggtagtc cggcgcagcc
    12541 tccggaatca ggatgcggta ggattcgagg gattgccagt gctcgacggc ggaggtcact
    12601 ccgccctgca ttccgctgag gcaagcccgc aagtcttcct gttgtgcctt cagaggcatc
    12661 tcgaggagcc gctcgaagag ttcctcgtga cattgttcca gggcttgctg cacggcagcc
    12721 gccggataaa tcgcttccag tcccgcatgt tcatagcgcc cgctcgatag atcccgcagc
    12781 gacgccaaat acgtcaggcg tgcaaaaatc cccggaatcc cggagagaga cgtcaccgtg
    12841 aaatcgtgga tgatcctgcg gttctgctgt agctgagtca agattcccaa gcgaagccct
    12901 catcccctgc atgttccgga gactgaacgt ttcatcgcaa gttcctcgct cccgcgcgtc
    12961 cccaagtcac catgggcgca aggaggaatt catcgatgaa aaagattact ctcgcagttg
    13021 caactctcgc acttctcgcg cttatcctac tcccgcttct tgggtcggtc aattactctt
    13081 tcaacaactc cgggtcccag agagccgatg gctccccttt ccctttgccg cccggacctc
    13141 cgatcgacgg cccgtctgtg ctcgtggccg atggctcccc attccctttg ccgcctgggc
    13201 ctccgatcgt aagtcctcga gcttcgtgac cgggtcaaat ctcatggggg ccggctgaac
    13261 ccggctggcc tcctagtctc tgtcccccgt agaattcata gtgagcttac tttactagcc
    13321 cgccaatttc tgttacaatc cgtcgctgat gttcgacatg gcgttcacac aatacgccgc
    13381 ctgggcggca tgtcaggctg tgctcgtatt cctcctcgct ggcctcttgc gcagcgggaa
    13441 tcaccgccag ttccctgtct ttacagcgta catcctgtca aatctcgggc aggctgcgct
    13501 ccttgtgttc agttatcgtc agtggggccc gcaggcgccg gtcacccagc agattttctg
    13561 gatcaccact ggggtgactc ttctcgcgcg ggcgttggcc atcgcggaat tgatccaccg
    13621 catcctggga cttcacaaag ggatttgggg acttgcctgg cgatcgattg ccgtgagtgc
    13681 cgttgtgatc gcggcgtatt cgttccttgt ggcgaaccgg cgctgggatc tcgggttgat
    13741 cgccgctgat cgcgcgctgg aacttgccct cggctgcgga atcgtcgtgc tcttcgtctt
    13801 ctgccgatac tatgaggtgc aggtaccaag agcggagcgg atcgtcggca tggggctcat
    13861 cctgtattcc ggcgtcgtcg tgatcagcca cacattcctt gagagatttt tttatgcgta
    13921 tagccctttt tggaatttga cgatactcac atccttcttc gtgagcgcaa cgctgtggat
    13981 tcgggcccta tgggtgccgg taccgagcat gcgggcgcag cctgccatgc ttgggcagct
    14041 ttatcagact ctttcgccgg agatcaatct gcggctccgc gctctggacc aaaggctcat
    14101 ctccctctgg aaaagggagg cgccaaactc atgacttggg ctttgctcac tttcttcttt
    14161 ctcgccggcg gcctgctggt actcttcgca tggtctttgt accagccccg caaacatttt
    14221 ccatcaacat tacccgcggc ggtcaatgat gactcgcctc gccgccatgc accccatctt
    14281 gctcagataa gtcagtcgct ttctcccgag gatattgcgt ttctcatgac tcgcggttct
    14341 cgagtcatcg ccagacgagc ggcggctgag cgccggcaca ctgcgatccg ctacgtcgaa
    14401 gtcctgcacg aagatttccg cagtctcttc cagatgggac gcgttctggc ttcactttca
    14461 ccggagatca aagggcggga ggaggcggaa cggctttggc ttgcggcgcg gttcgagtgt
    14521 cgttaccaga tcgttcgcgc gatgctactg ctcagaggtt cacccgtgct gcaactccag
    14581 caaatgacgc agatcgtcgg aaggctctcc cgaaggctgg acgccgagat ggcggaatgg
    14641 ggagagcgta ccgctctggc gatggagatg ggttcagtcg ctgatcgaag gcacccggac
    14701 acgttttaga gtctcttcaa gagtcgcaat caattcagca gttttgaatg gcttttgcag
    14761 cacgcggctg cttgaaaacg gctgcgggcg cacggcctcg gccgggcccg tccccgagct
    14821 catcaagacc acgcgcccga ccaaatccgg tttcgcctcc gccagccagt gaagtaaatt
    14881 ctccccctcg cgccttccgg gcagatgcgt atccgccagg atgaggtcga aatcctcagc
    14941 cataaggtat tccttggcgc gatccgcagt ctgggccgtc tggacgaaat gtttcctggc
    15001 gcggagtgtt tcgctcacgg cttccagtac tgaatcatcg tcgtcaatca gaaggattcg
    15061 gcgacgcttc cccgccggtg ccacatcgcc gccggccggc tgttcgatct cggccttgtg
    15121 ctgccagggg aacgcgatcg taaatgtggc gccgcgcggc ggcgcattcc ggacctggat
    15181 ggttcccccg tgctccatga cgatgccgta acaaatgctc aaacccaggc cggtcccctt
    15241 gcccaccggc ttggtcgtat agaaggggtc gaatacccgg gcaggatctt ggacacccgg
    15301 cccgctgtcg gcaaactcga tgatgaggtg gtcttcttcg gaagccgtgc gaacccagat
    15361 ctgtccttcc cgcgagtgct ccagaatcgc gtccaccgca ttgttcacga gattgagaaa
    15421 cacctgctgc aattggtgcg gatccgcctc cgtcacggga aggtccgaag caagatccaa
    15481 gttaagccgg atattgctta ggcggagatc ataatcccgc agggcgaggg tatccacgat
    15541 cacctggttt atcgcgaccg gtacgcgttc gggtttgtgt tgccgggcga aactcagcaa
    15601 gttttgaacg atacgatgag tgcgctgcgc ttgtttgtag agtttctcgg agtactccac
    15661 ggcctgcggc ccgatctgcc cgttcgcaga gagcaactgg ctatagccga ggatcgccgt
    15721 cagtggattg ttcagctcgt gggccacacc cgaaatcagt tgtccaatgg ctgccatctt
    15781 ctcactatgc aggagttgct cctgcgttcg ccgcaagttt tcataagctt gccgtgtctc
    15841 ctcgaacaga agcgaccgtt cgatcgcgct gctgatctgg actccgaccg caatcagcag
    15901 attcacgtca gcaggggaaa actcgcgcgg gttgcggctg cctacaaaaa gcccgccgat
    15961 cacacgatct ttcgaccaca ggacaaccac gtatgcggtc acgataccgt cttcgctttg
    16021 tgcttcgcgg aatgccggcg gcaacggcag gcccaatgtg gagagaaacg tggctcggac
    16081 tgcgcggacg tgttgcaaca agtccggctg aacgggaatg gaggaatgga ccctggaatg
    16141 cccgctctgt tgtcccacgg cagcgacgca ctgcagcatg gtttgtttct cgtccagcag
    16201 gtaaagggaa cttgcatcca actggaaaag ctccacaagc tgacgcagag tgcggcggac
    16261 ggaatccttg aggtcgagag actcattgag agtctggccg atggagttca acaccatcaa
    16321 ttcgcgattg cggcggcgga tttcctgttc ggcgcgcttc cgttccgtaa tgtcgagcag
    16381 gaaaccctga taggccgtca cttctccggc tgcgtcgcgg atcccaatgg acgattcgag
    16441 gacggttcgc acttcgccat ctttacgcaa ccactcgaac tcgaaatccg aaacgcttcc
    16501 atgctcgtgc agcagctttt tcaggcgttc acggtcggcc ggattcgcgt acagtgaggg
    16561 gatgttggcc cccatcaatt cttccctgct ctcatagccg gccattctca tcagggcatc
    16621 attgaaatcc gtgaatcttc cctgcggcgt agagatgaac acgccctcct ggacgtttga
    16681 aatcagagac cggtatttct cttcggcccg cttgcggtcc gtgatgtcct tcagaacatg
    16741 gaccgtgcca agctgtcgtc cttgaggatc cgtaaacgtc gagttggagg cgaggaaata
    16801 tcccccgagc cacggatcgg gatcgtcgcc ccctccggcg ctcccttcgc aataggggca
    16861 ttggtcgtaa ttccctgccc gtcttggaaa gatttcgccg atcgatcggc cgtacatcgc
    16921 ggatggatgc cgcccgagga tctgtgaaaa gcgttgattg tttctgacga tgcggtattc
    16981 cgggtcgtgc accaaaacgg ggtcaccgat ggagtcaaac gtgtacgccc attgcttctg
    17041 ggctgtcgcc gcctgctcaa agaggtgcac gttcaggagg gtaaggccca ataagtttgc
    17101 tacattcccc aagaaggaga tttcatcctg ggggaacttg acgggatgcg acgatcccag
    17161 gaccagcaat ccctgcggcc cgttcttgcc ggggagcggc acggtcacga gttgcgtgat
    17221 gccaccttct atcatccggt tccgcgtgcg ctcgtccgtt tcatcatcca aggtatgtgc
    17281 tacaagacca acgctaagga ccctctgcac ccacggctca cgaacaggca ggcggtcgta
    17341 tttctcattg aaggccgggg tgaagcccac ggagctcttc agcaccaatt ctgccgagcc
    17401 ctcactccct tcgagcagcc ggactgcccc atgcgtcgcc gccaggctcc ccaccaggct
    17461 taccagaacc ttgtccagca cctccttgac ggaaaaggtt tgcgtgctcg cagttgccaa
    17521 aagggtgagt cgccgcagtt tgtcattcag ctcctctgcg cgtgccctgg cgccttcgag
    17581 cacgaatacg atcatcccca tacccaaaac cacgaaaagc aaatcgccga aagccagacg
    17641 cacaagatac aaagggtgct caggccagtc agggcggtcc aacccgtgga ggccaatcag
    17701 aaagaatgcc cccgccagga acttggcgct gaccccacga gtcgccacgg tacagcgcca
    17761 cagcacccac ccggccgcga tggcgaggaa actttccagg gctgcggttt cccagcgcag
    17821 cttgccgaac tgcgccggca ctttcgcctc caggtacaaa tcaccgagaa ttgcgaggca
    17881 ggccaacgga atcaagggcc atcgcgccgg agctgaaccc gagataagct gagtgacacc
    17941 agccagaaag agcagaaagg ccgctatctc tccggtgacc gtaatgattc gcaggaaagg
    18001 cagaccgccg gagtaaacca gggaggcgag cccagcaaag ccggttaccg tcaggacgac
    18061 ccaacccgcc aaccaggtgc ggagatacgc gaggcggtaa tctcgctgca gatagaaaaa
    18121 aagcaccaga aaaatgaacg aggttgtggc ttcaaagaaa gcgatgccca acaatccctg
    18181 gttttgccag acgataacgc gcatcctttc cggatcgtcg tgccccggca gagtcggctc
    18241 cgccacagcg cgcgccagtc cgcgcggagc agagagagaa gcctgcactt aacgctaaca
    18301 cgctgttttc caactgagca agacgggcaa agctggaatg tgctgtccct tataatggct
    18361 gtgggagcgc ggaactgtgg aaccctccaa aatactgatc gtggacgacg aagagtcgat
    18421 tcgagaagtc gtcgccacca tgctcagagc ccagggattc cattgcgttg cggtttgcaa
    18481 cggccgggag gcccaggacc aagtcctgaa aatgcctccg gacctggtac tcagcgacat
    18541 gatcatgccg gagatggacg gcgttcgcct tcttgaatgg ctgcgccagt atgacccgga
    18601 tattccggtc atcatggtca ctgccattca cgatatctcg acggctctcg aagccatccg
    18661 tcgcggcgcg tacgactaca tcctgaagcc tttcgagaag gaccagctct accacgccgt
    18721 cgggcgcgcc atccagcacc ggacgcttgt gcttgaaaac cacagctacc agcgcaacct
    18781 cgaagttctc gtggaagagc ggacggcaca gctttccggt acactcgccc agctggaaca
    18841 atcctacgac gacaccctcg aggccctggg tagcgcactt gacctccgcg acgccgaaac
    18901 cgagggccac tgccagcgcg tgaccgcgtt cacggtctac atcgccaaga ccatgaatgt
    18961 cccgcccgag gatctgccca ttctggcgcg cgctgctttt ctgcacgaca tcggaaagat
    19021 ggccattccc gacgtgatcc ttcgtaagcc cggcccactc gacgatgatg aaaaacggat
    19081 catgcggacg cattgcgaga tcgggtacaa catgctgatt cggatcccgt ttcttcggac
    19141 cgccgcggaa atcgttctgg ctcatcagga attcttcgat ggcgccggct atccccgcgg
    19201 cctccgcggt gaagcgatcc ctctcggagc gcgcatattc gccgtggcgg acgctctgga
    19261 tgccatgctt tccgaccggc cttaccgccg ggcattgccc ttcgaccacg cgctggcgga
    19321 aatccagcgt tgctccgggt cgcagttcga tcccaaagtg gtggaggttt tcctgtccat
    19381 cccgcactcg cattggaccg aaatgcgcca gaatatggat tcccctttcc gtctggcgca
    19441 cctgcagaat ctctagcgga accggcggcg cggggactac gaggtcttgc acggcggagc
    19501 gggctcaaag taaactcagg ccgctgaggt ttttgcatgg cgctcgtccc catcaagacg
    19561 gcttcggaaa cagggtattg cgactgcgtc tgcgggtgct acgaactcgc cagtttgcgc
    19621 gacgaagata ccggcaaggg cttctgccca aactgcggaa agcagcacta cgaactcaat
    19681 gccggcgtcg gcattcctca gccggctccg gcaagagttt ccgccaattc ctccgggaca
    19741 gccctcgaca ctccagtacc tactgccgcc gatcccccgg gccgcctgcg ctgatccgga
    19801 aaccacgcgg gtcgagcgga caagaataca gttcggcggt atcgccgggc aaaacagcgg
    19861 gacaaggaat cacgcgcagg cggactgctt ctgacgtata attcttcgtg caagcagaac
    19921 ttctcgacag gggatgagta tgaatcttaa gtctctgaca ggtacgatcc tggcgggcgc
    19981 cctttgcctc agcgcttacg ggctgaccgc ggcgcagcag aagcaggaaa agattgcgca
    20041 cacgaatctg aaggtgggag atgtggcgcc ggatttcacg ctgctcgaca acaagtggaa
    20101 gccggtgcat ttgagtgatt accgggcaaa gaaaaatgtc gttctcgcgt tttacgtcct
    20161 tgccttcacc ggcggttgaa cgaaagaact ccaggcctat caggctgata aggccaagct
    20221 cgaggcatcg gatacggtgg ttttcggaat cagcatagac agtcccgcag ccaacgacgc
    20281 cttcgccgag aagatcggcg taaccttccc cctgttgagc gacatgaacc gcaaggtgct
    20341 gaagcaatac ggcatcctga aatcgtacaa cgtgcaaagt gacgactacg agtgggcgca
    20401 gcgcacgacc ttcgtgatcg acaaagccgg agtcatccag cacgtggagg aaggcaacgg
    20461 cgcggtcaat cccaacaatg ccatcgcggt ctgcacctct ctgccgaaga aataaacgct
    20521 tcggcattcc ggcgcccgcg tgccaggccc gatgaccacg cgacccgtcc cgtcgaaagc
    20581 aggaccggcc accgagccga tcccgcagtt cgatctgcac gcccagtatg cggcgattca
    20641 gggcgacatc cgcgcggcaa tcgaggacgt tttttcctcc cagcagttca ttctcggacg
    20701 gcaaggcgag gcgctcgaac aggaaatcgc aggggtttgc ggcgttgcgc acggcgtagg
    20761 cgcagcttcc ggcacggacg ccctgatcct cgccctgcgc gtctgcggag ttggcgcggg
    20821 cgacgaggtc cttctgcctc ccttcacctt tgtggccacc ggcagcgccg tgagcgcgct
    20881 cagagcgcgc ccggtgtttg cggacatcga cgccgcaacg tttaatctcg atccccatga
    20941 actcgaaagg cgcatcacgc cacggacgcg agccattgtc gccgtgcatc tttacggttt
    21001 gcccgccgac atggacccca tcctggcttt cgcgcgagag catagtttgc cggtaatcga
    21061 agatgcggcg caggcgatcg gcgcgtcgta caagggccgc ccggttgggt cgctcgggga
    21121 tgcgggctgc atcagcttct atcccacgaa gaacctcggc gcctatggcg atgcggggat
    21181 gctggtgacg aactcggcgg agatcgcgaa gcgtgcccgg aggctgagga atcacgggca
    21241 aacggggaaa tacgtgagcg acgagccggg attgaacagc cggctggacg aattgcaagc
    21301 ggcgatcctg cgcgtgaagc tgaagcatct gccggaatgg cagcgcgcgc ggcagcagca
    21361 tgccgcggaa tacagccggc tgctcgaggg gattgcaggg ttgacgacgc cgcgcgtgcc
    21421 cgcgggatgc gagcacgtat atcatcaata cacgattcgc ctaccggaga gggaccgagt
    21481 gctgaagacc ctcgcggagc agaagatcgg cagcagagtg tactacccgg tgccgctgca
    21541 cctgcaaccg ctctacgcct cgcttggcta taagccgggc gacttcccgc actctgagcg
    21601 cgcggcccag gaagttcttt ccctaccgat gtatccggaa ctgcgagatg agcagatccg
    21661 ccgcgttgct gctgctctgg ccgaagccat ctctcctagt tgaattgaaa ggtaaagccg
    21721 cggggtggaa ccgcgcagct attcggacga atttgcgcgg cggacggcgt ggtggaggac
    21781 tccgacgaac aggaagagcg tgggaatgag gagaaaaaga actccgtggc caatggcgcg
    21841 agaggccttg gccccggcct gggcggcctg gtcatagcag agggggcagg attgggcgaa
    21901 aacagaagaa gaaacaaatg cagaggcgca gagaatagag aaaaagcggc gcaatgaagg
    21961 ctttctgaag atggcgagca agccccgact cctcgactgc gggcctgcaa gagccatagg
    22021 ccctcctctc gggacaacag ccctatcgcg ctcttgcttg ggcgtcgcgg attggaagag
    22081 atgcggcatg tttctcactg agcgcctcag tgcatgaccg gattgccgag gcggaggcta
    22141 tccgggaaga aaaagacgca aatcatcata acgcaaaaca tgaatgcggg gacaagcaaa
    22201 agtacaagac cgaacttctc atacttcagg tgcatgaagt aggccatgat gaggccggcc
    22261 ttgacgatgg aaaggccgat caggacggtg agcatgatgc cgggggcgat ctggttgtat
    22321 cccagaaaca cctcgattcc ggtgagggca acgaggccaa accagacgat cacgaacagg
    22381 ctggacttgg cagggacgtg cccggcggtt tctgcattgt cgtgtgcgct catctcgtgc
    22441 tcctagtatt ttatggacat caggtagacc agcggaaaaa cgaacatcca gaccaggtcc
    22501 acgaagtgcc agtagaggcc gctgacttcg acgtcctcag cggagaattt cgatctgccg
    22561 aaccccaggg cgacgatgcc gaggtagatt acgccgatgg cgacgtgggt catgtgcagt
    22621 ccggtgacgc cgaaaaagac tgcgccgaaa acgggcgagc cccaaggatt gccgaatggc
    22681 cgcagccctt cttcgatgag gtgcagccac tcgcgcaggt ggaggccgat gaaggccgca
    22741 ccgaagaaca tggtggccag aatccatttg acggccgtac cgcggtcgcc gcgcgccgcc
    22801 gccgccacgc cgaagaccat ggtcaagctg ctggcgagca gcacgacggt catcaccgtg
    22861 gaaaagacga tggacggtga aaacgggaac ggcctgggcc aatcgtggtt ggccaggcga
    22921 agatagctgt agctcaacag cagggcggag aaggtgagcg agtcggagat gatgaagagc
    22981 cacatcccga gcttcttcga gcccatggcg aacggccgcg tgccgcccga ccaggccgag
    23041 ctcaccggcg cctcttcatg cgcatggtcc acagttcacc ttcccaggta gaggagtgcc
    23101 atcagaaaaa cccacaggcc gtccataaaa tgccagtaga tggaggcgat ttccgctgca
    23161 acggcgcgcg tgacttttgc gcgttcaaag tgcc
//
LOCUS       DASYVO010000227        11405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_106682, whole genome shotgun sequence.
ACCESSION   DASYVO010000227 DASYVO010000000
VERSION     DASYVO010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 11405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11405
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106682"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..920
                     /locus_tag="VGR03_07530"
     CDS             345..920
                     /locus_tag="VGR03_07530"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HEV1994164.1"
                     /translation="MKKNRMLLMIAIAAALAAMCLPLRAGRQEKSGEKTPAAKPGAAE
                     MERLKFYLGEWDYTETYPKSASYPNGGKNTGVYTSKLGPGGNSLINTFHSQGPVGDFE
                     GLLVMTWDPKEKAYKAYVFGNEFPGAIVETGQFDGDALVYGSEFPVEGATLKLRNITR
                     ITGPGTLVSEEFMTMKDAPEKLFVRVDAKKR"
     gene            complement(933..1679)
                     /locus_tag="VGR03_07535"
     CDS             complement(933..1679)
                     /locus_tag="VGR03_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994165.1"
                     /translation="MKPLLCSSLLLLTFSLNAQAPTAVPIPKEPHHHLVLENSYVRVF
                     RVSVPAHEATLLHQHDVPYVYVALGPADVINAVQGKPEARIVMADGQLGYSRGGFAHI
                     ARNESDAPFNNVTIELLMPQGNARNICAGVVPDAPGPGICEPPRLDARIAHSIILFKT
                     DELVAEHHSLGPKVRAPDSKSRKARLLIFDNDSPLRVQLPNKTLLTPKGSDVLWVEAG
                     RELKITNSNQSTASSYLVLSFKHADDAKIP"
     gene            complement(1807..3018)
                     /locus_tag="VGR03_07540"
     CDS             complement(1807..3018)
                     /locus_tag="VGR03_07540"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEV1994166.1"
                     /translation="MPEAVIVSAVRTPVGRAYKGSLRATRPDDLAALAIKAALARVPG
                     LDAKEIDDVILGCAMPEGEQGMNVARIAALRAGLPVETSAMTINRFCSSGLQAIAIAA
                     ERIRAGSAEVIVAGGTESMSMVPMGGNKISPNPWLVDNYPDAYINMGLGTENIARKFG
                     ITREQADQFSADSHKKALAAIAAGNFKDETIPVEVKITALPNGNGASSRPKSGTVKPT
                     TQTFTFSTDELPRADTSLDVLSKLKPAFHVKGTVTAGNSSPMSDGAAAAVIMSDARAK
                     SLGLKPLARFVAYATAGVLPEEFGIGPVQAIPKALKLAGLSLNDIAIIELNEAFAAQS
                     LAVIQQLGLDVARVNPNGGAIALGHPLGCTGAKLTATLIRELARRNARFGMVTMCIGG
                     GMGAAGIFERL"
     gene            complement(3737..4678)
                     /locus_tag="VGR03_07545"
     CDS             complement(3737..4678)
                     /locus_tag="VGR03_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEV1994167.1"
                     /translation="MISRIRLFVLPLLALALIGAACSKSPARSDSASSGKSGVRPTIV
                     FMTDFGTANDAVAICKAAMLSIAPDARIMDITHQVTPYQIEEGARFLEAVSPYYPAGT
                     VFVSVVDPGVGTSRKAIIVKSKKGQYFVLPDNGLITPVIDRDGLDSAREITNQNWMIQ
                     SAISSTFHGRDIFSPAGAHLAAGWDFNLVGPEVPQLVRLSPKTSTTSDKGIAGDIIAL
                     DDPFGSLVTDIPGDEFKKLGYNLGDKFRIEIDKKPVTLPYVKVFMDVPVGDSLLFIDS
                     RDRVSIAVNQGNYSKKFSVEPPGTIFIPRKGAPLKEK"
     gene            4815..5057
                     /locus_tag="VGR03_07550"
     CDS             4815..5057
                     /locus_tag="VGR03_07550"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1994168.1"
                     /translation="MWLIYRIVIGLIAGWLSGKVMKGGGYGVVVDIILGILGGVVGGF
                     VFDLLELHAGGPIGSIIVAFVGAVILVWITRLVKKA"
     gene            5339..5824
                     /locus_tag="VGR03_07555"
     CDS             5339..5824
                     /locus_tag="VGR03_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994169.1"
                     /translation="MKRNAGGKWLQITALLCIASMLGVASVAAIKNMAVVISAGSKLS
                     DVPLADLVKLCKGNQKTWSDGKSFTLVIKNPEAPEMRVAMQKLFGDAGGDVKAAIAKV
                     NESRLIVKIVGSDEELLRIVETTPGTVGILDVYSINSSVKVLRVDGKLPFDAGYALKG
                     N"
     gene            6511..6810
                     /locus_tag="VGR03_07560"
     CDS             6511..6810
                     /locus_tag="VGR03_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994170.1"
                     /translation="MKTQDKSRKWMWMFLAALVSLQMYFVWELLAVFALFAVGFAAIA
                     FVTGSLYMLHRGWALAVERIADSQHPVMVATRQGIYMIEDAARRPFRRPGSAPAR"
     gene            complement(7022..8554)
                     /locus_tag="VGR03_07565"
     CDS             complement(7022..8554)
                     /locus_tag="VGR03_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994171.1"
                     /translation="MSQSSLIDSPRNSVGEDARRSTRIERSVPLIVFGQNRMGEPFVE
                     RTVSTSLNLHGCRYPSRHDYGVGSWVTLQVVGLNVEPKPPAVRARVRSVHTSQSSREL
                     QQVGVELENPGNVWGVVTPPQDWMSARGTNTTLAQFATAVAPALDPPPMTVKLDEPPV
                     NAEHKMAEVATFPSPSPAASKPQAPKLAEAPKSQRVVITPDGLIAALQGKLQQAAEKA
                     VQAAVAKQVGEALREALSSIDDVRKSSVREIQELFPARVEAMRLSSKEEFAGEIASHW
                     KEQMEKYRGQAEEMAQRLEKQAAELRRELARSQEFVERMTREREPQIHARLNEEVAQA
                     TSQFETATARAADRRYQVMLENTQAVTQEALLKLDARSAEVQALVQSAVNSALGAFQR
                     QTDQHVNAVLSETKERAVSALSSLEAESRVTCEARRLALETEVARAAERSTDQFRKGM
                     KAFLYSCLVAAVSAVDEHSKSTLDGLVKDNGKTLFEAESDSRTQDDPEIIPDTDIDPL
                     TH"
     gene            complement(8821..9603)
                     /locus_tag="VGR03_07570"
     CDS             complement(8821..9603)
                     /locus_tag="VGR03_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1994172.1"
                     /translation="MELQDRVALITGSGRGIGRAIAYLFAKEGADVFLTARTEKELAS
                     TTAAIAKGGGRAGYATADLSREADCARAVAAAREKFGRIDILVNNAGHYGPVVPVEDY
                     PLADFDNVIAVHLRAAFLLSKLVLPEMYARKSGVILNISSLSAKAAYAWGSAYAAAKA
                     GMLGLTRVTAAEGARKGVRVNAICPGPVTETQMSKDLGAVLAKKMGVNEDEQLAGFLN
                     GLLQGRAQTAEEIARAALFLCSEQSSAMTGQSINVDGGVAFY"
     gene            9854..>11405
                     /locus_tag="VGR03_07575"
     CDS             9854..>11405
                     /locus_tag="VGR03_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994173.1"
                     /translation="MCRTILAFHLTAVLAACSLAGCSALSNPGGGTNPVALSLTVQPG
                     SVTVTAGQAVTFSVSASGTAPISYQWRKNTVDIGGATASSYTTPVTTTADNGAKFDVV
                     VSNSVSSVTSSQATLTVNSTPVAPTITTQPANQTVTAGQTAMFSVVAAGTAPLTYQWQ
                     KNGSVIPGATAASYTTPVTTTADSGELFRVVVSNSAGNVTSNSAMLTVNPGTGNSTVD
                     VITYHYDNGRSGMNLNETALTPANVNSTQFGKKGEFTVDGHVDAQPLYLSQVTIGTQK
                     KNVLYVATEHGSVYAFDADSISGTTSTFLWKTTTLGSGETTSDNRGCGQISPEIGITA
                     TPVIDRARNAIYVVAMSKNSSGTYFQRLHALNLSTGVELFSGPQTITATYPGTGDNSS
                     GGNVVFDPKQYKERPGLLQINGTIYTTWSSHCDFRPYTSWVMAFSAATLAQTSVLNLV
                     PNGSEGGIWMAGTAPAADAAGNLFFILGNGDFDTTLNASGFPANGNCGNCFVKLSTSA
                     GLKLADYFT"
BASE COUNT         2389 a         3121 c         3518 g         2377 t
ORIGIN      
        1 ctctctacgc tcaggccacg cagctcttga acctggcgct ggatgagagc ttcgccaacg
       61 atcctttctg gatcaaacca caagccgacc gtgccttctt ccgtaccatc agcatggaac
      121 tgttcccgcc gcagcgggcc cagcgcgcca aagcctaaac aacccagtct cgtctctcgc
      181 cccgaggccg cgaacagcgg tccccggggc gaactgtttg cgagggggcc cgccgaatct
      241 gacagtgccc ggcgtggtca cgatcccgag ggaaatacag tggagctgtg gaaggcggag
      301 aagttaaaat acgaatcgcg gccgatgtga gcagcggagg agccatgaaa aaaaatcgga
      361 tgctattgat gattgcaatc gccgcagctc tcgcggcgat gtgcttgccg ttacgcgcag
      421 gacggcaaga gaagagcgga gaaaaaacgc cggcggcgaa acccggcgcg gcggagatgg
      481 agcggctgaa attttatctc ggggagtggg actataccga aacgtatccg aaatcagcgt
      541 cctatccgaa cggagggaag aataccggcg tgtacaccag caagctcggg ccgggcggaa
      601 attcgctgat caacacgttt cattcgcagg gaccggtcgg tgattttgaa ggattgttgg
      661 taatgacctg ggaccccaag gaaaaagctt acaaagcgta tgtgttcgga aatgaatttc
      721 ccggagccat cgtggaaacc ggacaattcg atggcgacgc gctcgtttac ggctcggaat
      781 ttcccgtgga aggcgcaacg ctgaaactgc gaaacatcac gcgaattact gggcctggaa
      841 cgcttgtgag cgaagaattc atgacaatga aagacgcgcc ggaaaagttg ttcgtgcgcg
      901 tcgatgccaa gaagcgatag catcagcatt cgtcagggta tcttcgcatc atccgcgtgt
      961 ttgaacgaaa gcaccaaata acttgaggca gtggattggt ttgagttggt tatcttcaat
     1021 tctctacctg cttctaccca cagaacatca ctgcctttcg gtgtcagcaa tgtcttgttc
     1081 ggaagctgca ctctgagcgg ggagtcgtta tcaaaaatta gtagtctagc cttcctagac
     1141 ttcgagtctg gcgcccgcac tttaggaccg agcgagtggt gttcggcgac aagctcgtct
     1201 gttttgaaca ggatgatcga atgcgcaatg cgcgcatcta gccgaggggg ttcgcaaata
     1261 ccggggccag gtgcatcggg aacgacgcct gcacaaatat tgcgagcatt gccctgaggc
     1321 atcaagagct cgatcgtgac gttgttgaag ggggcgtcgg attcgttgcg ggcgatgtgg
     1381 gcgaaaccgc cgcgggagta accgagttgt ccatcggcca ttacgatgcg ggcttcgggt
     1441 tttccttgga cggcgttgat gacgtcggcg ggaccgagcg ccacgtagac gtagggaaca
     1501 tcgtgctgat ggagaagggt ggcttcgtgc gcgggaacgc tcacgcggaa tacgcggacg
     1561 taggaatttt ccaggacgag atgatgatgc ggttccttcg ggatggggac ggcggttggc
     1621 gcctgagcgt tgagagagaa cgtgagaagg agtagtgacg agcaaagaag cggcttcatc
     1681 cggcgtgacc tccgaacaat agagcaagcc aaaagaaaga tgcgagataa actcgccgct
     1741 acaagaacga aacgcgccga gttcaaacct gccgggctac aacccggcct ctacaaaaac
     1801 aagagttcag aggcgctcga agatgccggc ggcacccatg ccgccgccga tgcacatggt
     1861 gaccatgccg aagcgggcgt tacggcgcgc taattcgcga atgagggtgg cggtgagttt
     1921 tgcgccggtg cagccgaggg ggtggccgag ggcgatggcg ccgccgttgg gattgacgcg
     1981 cgcgacgtcg aggccgagtt gctgaatgac ggcgagggat tgtgcggcga aggcttcgtt
     2041 gagctcgatg atcgcgatgt cgttgagcga gaggccggcg agcttcaaag ctttggggat
     2101 ggcttgaacg gggccgatgc cgaattcttc ggggagcacg ccggcggtgg cataagcgac
     2161 gaaacgcgcg agcggcttga ggcccagcga tttggcgcgg gcgtcactca tgatgacggc
     2221 ggcggcggct ccatcactca tcggggagct gtttcccgcg gtgaccgttc ctttcacatg
     2281 aaacgcgggc ttgagtttgg agaggacgtc gagtgaagtg tcggcgcggg ggagttcgtc
     2341 ggtggaaaag gtaaacgttt gagtcgtcgg tttgaccgtc ccagacttcg gtctggagga
     2401 agcgccattg ccgttgggga gcgcggtgat tttcacttcg acggggattg tttcgtcttt
     2461 gaaatttccg gcagcgatgg cggcgagagc tttcttgtgg gaatcggcgg agaactggtc
     2521 ggcttgctcg cgggtgatgc cgaatttgcg ggcgatgttt tccgtgccga ggcccatgtt
     2581 gatgtaggcg tcgggataat tgtcgacgag ccagggattc ggagaaattt tgttgccgcc
     2641 catgggcacc atggacatgg attcggtgcc gccggcgacg atgacttcgg cggagccggc
     2701 gcggatgcgt tcggcggcga tggcgatggc ttgcaaaccg gaagagcaga agcgattgat
     2761 ggtcatcgcg ctcgtttcga cgggaagtcc ggcgcggagg gcggcgatgc gcgcgacatt
     2821 catgccctgc tcgccttcgg gcatggcgca gccgagaatc acgtcgtcaa tttctttggc
     2881 gtcgagaccg ggaacgcggg cgagcgctgc cttgatggct agcgcggcga gatcgtcggg
     2941 gcgcgtggcg cgcagcgagc ctttgtaggc acggccgacg ggagtgcgga cagcggaaac
     3001 aatgacggct tcgggcattg tgggacctcg aaaaacgaag agcctcaggc ctcccgaccc
     3061 ggtcgggaca ggttccggcc tgagctacag attcattgtt gggcgagcgt ggggaaaaag
     3121 caaccgtgtg gtgactggtg attcgtgact ggtggaagat agggctgttg gccccttctc
     3181 actggaaagt aaggagacat ctgtcgaggc gctgcgccgg atatggaaag gagcctccct
     3241 cgggtttctg gttaaccata atacccccct acccctatgt ttttcataag tgttgattct
     3301 aaagatagtt aaagtctttt gttttgatac acttttgcaa gtgttgattc taaagggact
     3361 tgcgttgcac caaaattgtg caaagtatgg cctgccgctc tgtttttcat aagtgttgat
     3421 tctaataaag ttagcgggac atgctggttc caccaaaaag aaaagcgcgg caggtagctg
     3481 ccgcgctttt ggatacgatt atccagtgcg tattgtaccg aatagctaat caaaaatcaa
     3541 ggggaattta ggccgcgggt gtgttttccg gatgtgccca gaggattccg gcaatctcgg
     3601 tacttagcta ctgaggggga tgggcgcgga gggagcgggc gatgaggccg agtccgccgg
     3661 gggtgccacc gacgtcactg atggaacggt tttccacggt ggcaggatag cagagactcg
     3721 gccggtgttg gaggccttac ttttccttga ggggggcgcc cttgcgcggg atgaagatcg
     3781 tgccgggagg ctcgacgctg aacttctttg agtagttgcc ctggttgacg gcaatgctga
     3841 cgcggtcgcg agaatcgatg aacagaagcg aatcgccgac ggggacatcc ataaaaacct
     3901 tcacgtaagg cagggtcacc ggcttcttgt cgatttcgat cctgaatttg tcgccgaggt
     3961 tgtagccgag cttcttgaat tcatcgccgg gaatatcggt gaccagggag ccaaagggat
     4021 cgtcgagggc gatgatgtcg ccggcgatgc ccttgtcgct ggtggtggaa gttttcggag
     4081 aaaggcggac gagttgagga acctcggggc cgaccagatt gaaatcccaa ccggcggcga
     4141 ggtgcgcgcc tgcgggagaa aaaatgtcgc ggccatgaaa agtggaagag attgcggact
     4201 gaatcatcca gttttgattg gtgatttcgc gtgcggaatc caggccgtcg cgatcaatca
     4261 cgggggtgat caggccattg tcgggaagca caaaatactg gcccttcttt gatttgacga
     4321 tgatcgcttt gcgcgaggta ccgactccgg ggtcgactac cgacacaaaa accgttcccg
     4381 cgggatagta aggagacacg gcttcgagga atcgcgcgcc ttcttcgatt tggtagggcg
     4441 tcacctgatg ggtgatgtcc atgatgcggg catcgggggc gatgctaagc atggcggctt
     4501 tgcagatggc cacggcgtcg ttggcggtgc cgaagtcggt catgaagacg atggtgggac
     4561 gcacgccgga tttgccggag gaagccgaat cactccgtgc gggagacttc gagcaggccg
     4621 cgccaatcag agcaagtgcg agcagcggaa gaacaaataa acgaatgcga gaaatcatca
     4681 acactccgtg acggttttgt agttttcttg cgagccagtg taagccgcgg ggttgacatt
     4741 cggcaaggcg cgttctacaa gagacgcgcg gcgaggggag ctattctcgc agggagaaag
     4801 cggagggggc aaatatgtgg cttatttacc ggattgtgat tgggctaatc gccggctggc
     4861 tttccggaaa agtaatgaag ggcggaggat acggcgtggt ggttgatatc atcctcggga
     4921 ttctcggggg cgtcgtaggc ggcttcgtgt tcgatctgct ggaactccac gcaggaggac
     4981 caatcggctc gattatcgtg gcgtttgtcg gcgccgtaat tctggtatgg attacgcggc
     5041 tggtgaaaaa ggcctaacga agtttttcgg aggaaaaaaa gcgttgctac ggcaggagag
     5101 tcggccgtgg cagcgcgttt tttgttcgta gaaaggtcta gtgggatgtc ccggcgtctt
     5161 ggtacgcaac caggaaccat tcgacggtcg gggcgcgctg atatcttagg cgcgcgcgaa
     5221 tcgaggaaaa aggaggggac gcgcacagtt gagcacaagg cgaaaagccg ctacttgctt
     5281 cgtaccgtga cgagtgaaca tactgcaagc aatctacttg aaagtgagca tggaagttat
     5341 gaagcggaac gccggcggaa aatggctgca aatcacggcc ctattgtgta ttgcttcgat
     5401 gctcggtgtt gcgagcgttg cggcgatcaa gaacatggcg gtggttattt ccgcagggag
     5461 caagctctcg gacgtcccac tggccgacct ggtgaagtta tgcaagggca atcagaagac
     5521 ctggtcggac ggaaagagct tcactctggt catcaagaac cccgaggcac ccgagatgcg
     5581 cgtggcgatg cagaaactct tcggtgatgc cggcggtgac gtcaaggcag ccattgcgaa
     5641 agtgaacgag tcccgcttga tagtaaagat cgtgggcagc gacgaggagt tgctgcgtat
     5701 cgtggaaacc acgcccggca cggtgggaat tctcgacgtt tattccatta acagctcggt
     5761 gaaagttctc cgcgtggatg gcaaactgcc gtttgatgcg ggatacgcgt tgaaagggaa
     5821 ttagggagcc ggctgccggg tccctgtaag aacacctcct tccgtcggct gaacacgccc
     5881 gaacctgcca cgaagcctct cattcgatca ccaagtccgt gcatcgtcgt caaccctcga
     5941 gatagctgtc gtctgccgtt tctaatgtag gtcgtttcaa ccagctacgt tgcgtcctct
     6001 cattgggggc gaaacggcgt ataggggaag atccgagact cgtacccggc gtgcatcaag
     6061 atcccagaga ggcgccgccc cagcgacagg agcggtctgg aagactggtg agcggcggga
     6121 aatgaaacgt aaaggaacca ggaatcacta cggagagagc ccggaaatgc ctgtacgtag
     6181 gtacaggttt tccacagcta gtacagggaa aaagaggcga aaatcggggc gaaagtcgaa
     6241 tctcctgtaa catatttgtt ttgtattatt tacagtattt tgaactggaa acaggcggta
     6301 ctaggagact cgtttaggcc ccaaaatgtg tcctcggtac catcgccttt cgggcgcgga
     6361 aggtgtcaaa tgggacaggt acgagggatt cgattagacc ttctggacag ggtggcgcat
     6421 ctaagtcaga attgggtgaa gagcctagga agcgaaacac agagtagcgg ccagtgttaa
     6481 atagggcagg ggatcgggga tagagaaaag atgaagaccc aggacaagag ccggaagtgg
     6541 atgtggatgt ttctggcagc gctggtctcg ctgcaaatgt atttcgtgtg ggaattgctg
     6601 gcggtgtttg cgctgtttgc cgtaggattt gcggccatcg cttttgtaac cggaagtttg
     6661 tacatgctgc accgaggttg ggccttggcc gtggagcgga ttgccgacag tcagcacccg
     6721 gtcatggtgg ccacgcggca aggcatctac atgatcgaag acgcggcgcg ccggccgttt
     6781 cggcgtccgg gttctgcgcc agcccgctaa gtttttgacg accttctggc tacggccgga
     6841 gcacacacag aacctgtcac cctgcctcct tcaggactga cataagtttt gtggcaagag
     6901 tacgaggaaa agggccaacc gtcctgagag agcggttggc cttttttctt ttgtggggat
     6961 aggagatctg gtgccgcagc cgtctcctta ttattcttcg gctgcgccgg gcaactgtct
     7021 ttcaatgggt caggggatcg atgtcggtat caggaataat ttccggatca tcctgagtgc
     7081 gcgaatcgct ctccgcttcg aacagagtct tcccattgtc tttgacgagc ccgtccaggg
     7141 tggatttcga atgttcatcc acggcactga cggcggccac cagacaggag tacaggaaag
     7201 ccttcattcc tttgcggaat tgatccgtgg agcgctcggc ggcacgggcc acttcggtct
     7261 ccagtgccaa gcggcgagcc tcacaggtga cgcggctctc cgcctcgagt gaggagaggg
     7321 cggacaccgc tcgctccttg gtttcggaca ggactgcgtt tacgtgctgg tccgtctgcc
     7381 gctggaaggc ccccaaagcg gaatttactg cgctctgtac cagcgcctgc acctccgcgg
     7441 agcgcgcatc gagcttcaag agcgcctcct gcgtcacggc ctgggtattc tccagcatga
     7501 cttgatagcg gcggtcggcg gcgcgggccg tcgccgtttc gaactgggat gtagcctggg
     7561 ctacctcttc attcagtcgc gcgtggatct ggggctccct ttctcgcgtc attcgttcca
     7621 caaattcctg cgacctcgcc aactcgcgcc gcaactcggc cgcctgtttc tcgagccgct
     7681 gcgccatctc ctccgcctgg ccgcggtact tttccatttg ttctttccag tgcgaggcga
     7741 tctcgccggc gaattcctcc ttcgaggaaa gtctcatggc ctccacccgc gcgggaaaaa
     7801 gttcctgtat ctcgcgcacg ctggacttgc gcacatcgtc aatcgagctg agcgcctcgc
     7861 gcagcgcttc gcccacttgc ttggctaccg ccgcctgcac cgctttctcc gccgcttgct
     7921 gcagttttcc ctgcagcgcc gcgatgagtc cgtccggggt gatgaccacg cgttgcgatt
     7981 tcggggcctc ggccagcttt ggggcttgcg gctttgaagc agccggcgac ggggatggga
     8041 atgtggcgac ctcggccatc ttgtgctcgg cgttcaccgg cggttcgtcg agtttcacgg
     8101 tcatgggcgg tggatccaga gcaggcgcga cggcggttgc aaattgcgcc agagtcgtat
     8161 tcgttcctcg ggcgctcatc caatcctggg gcggcgtaac aaccccccaa acattccccg
     8221 ggttttccaa ttccacgccg acttgctgca gttcccgcga actctgcgac gtgtggaccg
     8281 agcgcactct cgcgcgcacc gccgggggct tcggctcgac atttaatccc acgacttgca
     8341 acgtgaccca cgatccaacg ccgtaatcgt gccgcgaggg ataccggcat ccatgcagat
     8401 tcaggctggt cgagacggtt ctttccacga acggctcgcc catgcggttc tgaccgaaga
     8461 caatcagcgg gacggaccgc tcgatgcgcg tactgcgccg cgcgtcttcc cccacggaat
     8521 tgcgagggct atcaatcaga gacgattggc tcacgtccgc tccaaagaga tgcagttcct
     8581 aatcccggta gctccagaaa actgctgtct ggcagagata tcttttccac ggggaggcaa
     8641 attcgatttg actgtgaacc aacttccggc acttttgagc acctgtgccc ctcgcggaac
     8701 cccacgcggc aaatgttagc cgaagctcgt tccgttgtcg atgcttgcgt ggatgcggca
     8761 tagataccca agaaccaaat ccacacggcc gatttgcact tcgacgcatt ccaaaatctc
     8821 tcagtagaat gcgacgccgc catcgacgtt gatcgattgg ccggtcatcg cgctggattg
     8881 ctccgagcac aggaacagcg cggcgcgcgc aatttcctcc gccgtctgtg cgcggccttg
     8941 cagcaggcca ttcagaaagc cagccaattg ctcatcttcg ttcacaccca ttttcttcgc
     9001 gagaaccgcg cccagatcct tggacatttg cgtttccgtc actggtcctg ggcagatggc
     9061 atttacgcgg acgcctttgc gcgccccttc cgcggccgtg acccgcgtca gccccagcat
     9121 tcccgccttc gcggcggcat aggccgagcc ccaagcgtag gccgccttcg ctgagaggct
     9181 ggagatgttc aggatcacgc cggactttcg cgcgtacatc tccggcagca ccagttttga
     9241 taacaggaac gccgcacgaa ggtgaacggc aatcacatta tcgaaatccg ccagcggata
     9301 gtcttccacg ggaaccaccg ggccgtaatg ccccgcattg ttcaccagaa tgtcgatccg
     9361 gccgaacttt tctcgtgccg ccgcaacggc ccgcgcgcaa tccgcttccc gcgataaatc
     9421 cgccgtggcg taaccggccc gtcccccgcc cttcgcaatc gccgccgttg tggaggcgag
     9481 ttccttctcc gtgcgcgccg tgagaaacac gtccgcgcct tctttcgcaa aaagataagc
     9541 gatggcccgg cctattccac ggccggaccc agtaattagg gcaacacgat cttggagttc
     9601 catagagaaa acgaggatag agtgaaccag gaaaggaggc aagccgggag gctcgaaatt
     9661 cgattgcggt ttggcattgc ctgctggcca acaactgtcc tttaggtcag gtgcaatatt
     9721 gctttgcggt gagtaacctt tatggcataa ccgggtaatc ctggcgccgg caagaccggt
     9781 gcctggtttt gccccttcct gcagttgcgg cctccccaag tccagccgct caaatcgaac
     9841 tggggtgtag ctcgtgtgcc gaacgattct ggcttttcac ctgactgccg tcctggccgc
     9901 gtgttctctg gcaggctgct cggccctctc gaatcccggc ggcggaacca atccggtcgc
     9961 gctgtcgctg acggttcagc ctggcagtgt cacggtcacg gctggccagg cagttacgtt
    10021 cagcgtatcg gctagcggca ccgcgcctat ttcttaccag tggcgcaaaa ataccgtcga
    10081 catcggcggc gcgacagcga gcagttacac cacgcccgta accactaccg ccgacaacgg
    10141 cgccaaattc gacgtggtcg tcagcaatag cgttagcagt gtgacgagca gtcaggcaac
    10201 gctcactgta aattccacgc ctgtcgctcc cacgatcacc acgcagcctg ccaatcaaac
    10261 ggtcaccgcg ggccagacgg ccatgtttag tgtcgttgcc gccggcaccg cgccgctcac
    10321 ctaccaatgg cagaaaaacg gctctgttat tcccggtgca accgcggcga gttacacgac
    10381 gccggtcacc accactgccg acagcggcga acttttccgc gtcgtcgtta gcaattccgc
    10441 cggaaatgtg accagcaatt ccgccatgct cactgtcaat cccggcacgg gcaactcgac
    10501 cgttgacgtg atcacctatc actacgacaa cggccgctcc ggcatgaacc tcaacgaaac
    10561 ggcgctgact cccgcgaatg tgaattccac gcagttcggg aaaaagggcg aattcaccgt
    10621 cgacggccac gtcgacgccc aaccgctgta tctttcgcag gtcaccatcg gcacgcaaaa
    10681 gaaaaatgtt ctttacgtcg cgaccgagca cggctccgtc tacgcctttg acgccgactc
    10741 catcagcgga acgacttcca cgttcctctg gaagaccacc acgctcggca gcggcgaaac
    10801 caccagcgat aatcgcggct gcggccagat ctcccctgag attggcatca ccgcgacgcc
    10861 ggtcatcgat cgcgctcgca atgccatcta tgtggttgcc atgtcgaaga attccagcgg
    10921 cacttatttc cagcgcttgc atgcgttgaa cttgtccacc ggcgtggagc ttttcagcgg
    10981 tcctcagacc atcacggcga cgtatcccgg cacgggcgac aacagctccg gcggaaacgt
    11041 cgtctttgat cccaaacaat acaaggagcg gcccgggctt ctgcagatca acggaaccat
    11101 ttacactacg tggtcttccc actgcgactt tcgtccctac acttcgtggg tcatggcttt
    11161 cagcgcggcc acgctcgcgc aaaccagcgt cctgaatctc gtgccgaacg gaagtgaagg
    11221 cggcatctgg atggcaggca ccgcgccggc ggccgatgcg gccggcaatc tgttcttcat
    11281 cctgggcaac ggcgatttcg acaccacgct gaacgccagt ggttttcctg caaacggcaa
    11341 ttgcggcaac tgtttcgtca agctttccac ttccgctggc ctaaagctcg ccgattattt
    11401 caccc
//
LOCUS       DASYVO010000228        11567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_106690, whole genome shotgun sequence.
ACCESSION   DASYVO010000228 DASYVO010000000
VERSION     DASYVO010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 11567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11567
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106690"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VGR03_07580"
     CDS             complement(<1..593)
                     /locus_tag="VGR03_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994174.1"
                     /translation="MTKSKFFKAAGVITAFVAIVFAGMVNSSRRVKAHDDDDDNEGNE
                     SKIRRGFEIAPVPLDLVGKNRSLVGLGSYIVNAQGDCDGCHSKGPATEFVVGGNPYFG
                     QKPTKVNPKTYLGGGRDFGPFPGPGPFPHIISRNLTPDKTGRAEGGNSFAQFKAIMRT
                     GIDADMVHPTCTGAPDGKCIPHPFDGSLLQIMPWPIYA"
     gene            969..3788
                     /gene="alaS"
                     /locus_tag="VGR03_07585"
     CDS             969..3788
                     /gene="alaS"
                     /locus_tag="VGR03_07585"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV1994175.1"
                     /translation="MTGNETREKSWSGNDIRKAFLDFFKSKGHTIVRSSSLVPHGDAT
                     LLFTNAGMNQFKDVFLGLEKREYTRATTAQKCVRAGGKHNDLENVGFTKRHHTFFEML
                     GNFSFGDYFKKDAIAYAWELITSPQWFGIPKDKLYVTVFEQSEISPGQVCEGDHDSLL
                     YWSNTDTDLPATRIRLLGAETNFWAMGDTGPCGPCSEIHYDMGPEASDLGHKDCPFSC
                     DCGRYVEIWNLVFMQFNRDASGTLTPLPKPCVDTGMGLERVAAVLERVISNYETDLFT
                     PLVDRAEKLCSSTLTTRPDLAASFRIIADHSRAATFLVSDGVVPSNEGRGYVLRKIIR
                     RALRHSRILGAPSSFLSKMSDAVRALMAGAYPELVETADRVNHILDEEETRFSRTVNI
                     GLKKLEEDLTELRGPDLIEHIAREPLRGTPFTVGQYPGDKAFRLYDTYGLPRDFIQDV
                     VRDATFSVDWEGFDRAMQEQRTRARASWKGGAKEAANPAYAKIADRFTTEPDFYFGTS
                     AKDCGIEAIILPGGAHRADTKSGPVNELKAGESGEIVLDRTAIYAESGGQVADTGAFY
                     DNSESQLLAEVTGAFYPVAGLVAHKVVAKETLRVGDRVAVVADAERRARIIRNHSGTH
                     LVNAALRNILGTHVKQAGSLNAPERLRFDFSHFAHVDAEELRDVEQQVNDEIRLNTEI
                     ETNVTSLEEALASGALAFFGDKYPEHNVRVVTIPDPRAARGFYSKELCGGTHVQRVGD
                     IGVLKIVSEESVAAGVRRIEAVTGIGALEHFQNQAEILRQVASKLNVGEDGILAQVEK
                     LSQTAKHLEKELEAQKRKGALSQIDELAARVQMVKGVKVIAAEVQNVDREGLRQLVDS
                     LRQKLGSGVVALGMPEDGKVALIAGVTKDLTAKVHAGKLIQALAKRVGGSGGGRPDLA
                     EAGGKDITGLKMALESVPSLLEPLL"
     gene            4083..4811
                     /locus_tag="VGR03_07590"
     CDS             4083..4811
                     /locus_tag="VGR03_07590"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEV1994176.1"
                     /translation="MIKRTAITARLDLAALVLCAAFFATFFAQGASGQIATVVDDGGR
                     RFFVNAERPLTAKLTATKLRTNIYLPAESSFSGRSRPAMDIGRDSVEKLVREAADRHR
                     MDPALVRAVIETESNWNPKAYSHKGAGGLMQLIPTTAQRYGACDVFNPQQNIDAGVKH
                     LKWLLERYNGNLDLALAAYNAGEGAVDRAHGIPAYRETRNYVQRVQNAYFRPGSGRLA
                     DAFINSHAIHRDLTPDGRIIFTNE"
     gene            4968..6968
                     /locus_tag="VGR03_07595"
     CDS             4968..6968
                     /locus_tag="VGR03_07595"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEV1994177.1"
                     /translation="MSFARLSPVFAALGLVLTLASAAPAKTNAEAAATAAVRRQSAST
                     QFARAEEQRAALNSKPTEKRTLADYKQVVTGYRRVALITPRAPEVPDSLLAIAELYTE
                     MGDRFGRSYYQSAADSYQFLVHEYPTSRYCQDAMLRAAKLQREQLGDSALAKKTYEDF
                     LKRYPRSPRKREAQEALAELALLQNGDETNIVAQNSTAASRAAEEPRANAPVHSGGKS
                     VSRSESSEIPRVRRIRAAATGEATRVTIDLEDSVQYASGRIANPDRIFFDLHAARLTP
                     EVARGNIRVDGGLLTAVRVAQNHDGVVRVVLDVNGVKDYTASLTNNPAQLLIDLYGNS
                     PAAPVQTAKGKKSQQDVKEDSVESAANSSGAGSNSQPSVKNAKSKSVKATATGAKPDL
                     IRPASAPQPTRDGQSTLTRALGLKIGRIVIDAGHGGHDTGTIGPTGLMEKDLCLDVAL
                     RLGKIIEQRLPGADVVYTRSDDTFVPLEERTNIANQAKADLFISIHANSSRDHAARGI
                     ETYYLNLKGSAEAMEVAARENATAQGGVHELQDLVMKIARTEKIDESKELAEDIQDSL
                     AKRIQKSSKPVKNRGVRKAPFVVLIGADMPSILTEISFLSNPADEQMLKKPEQRQKVA
                     EGLYQGVASYLESMNSVTMNLPVKTGAGHPAAAAVEQSRNQN"
     gene            6995..8098
                     /locus_tag="VGR03_07600"
     CDS             6995..8098
                     /locus_tag="VGR03_07600"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HEV1994178.1"
                     /translation="MRKYCLIFLSFLAAAMAASPRLTAQGALERAFPERFGRWKAVNS
                     PIKIKSEEGQAQVFAESGLTDSLAGEYSDGTQKVGVILRQFGDPSGAYEAYTAEFGPD
                     MHPSTVGGPSAIDRERLLLLLGNLILEVRPPEDPTTDELQQLAAMVRKHSDLTPLPPI
                     RAFLPPGFTDGTQRYARGPVAFRNAIAFLKQDEFANLVSEAGFNVNAEAMFAHYRAGK
                     NEAVLLLIEYPTPQLAEQHLRHLEQALGPAAKQEGTTIERKASLLSLVLRPSSAAYGN
                     ALRSAVNYETEVTWNEPHQTVSDPPLLTTVAKIFISTGVFMVVTVVLGVAFGGVRVLA
                     KIFFPGKVFDRPEQMDVLQLGLSGKRINSRDFY"
     gene            8286..9383
                     /locus_tag="VGR03_07605"
     CDS             8286..9383
                     /locus_tag="VGR03_07605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV1994179.1"
                     /translation="MLLALAAGAFVYGCQKSAADNAPGAGAPAMTVQVQIAPAVKIPD
                     TTEYLSILKSRHSAAINPQVEGQITRIFVKSGDHVEAGTPLLQIDPLKQEATVNSQEA
                     SRAAQEANLRYAKVSLERAQKLFDAGVISKQELDGAQTGYDASVAQVKSIDEQVRQQK
                     VELHYYSVTSPMDGIVGDIPVRMGDRVTVSTMLTTIDEPGALEAYIYVPAERAKNLKA
                     GLPVRLLDTAGNSVNETRITFVSPQVETETQTVLAKASIENSHSKLRVAQQVRAQITW
                     GVHEGPVIPVLAVLRINGQFFAFVSVNEGKGTVARQKPLKLGDTAGNDFVVLDGLKPG
                     DHLIISGTQFLQDGMPVTEQIQSNGKGVAGQ"
     gene            9440..>11567
                     /locus_tag="VGR03_07610"
     CDS             9440..>11567
                     /locus_tag="VGR03_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV1994180.1"
                     /translation="MFVDFFIRRPVFATVCALFIILAGAISIPTLPIAELPDLAPPQV
                     IVSSGYIGANAQTVESAVTIPLEQAINGVQGMKYISSTSGNDGSSQITVVFDVTRNVD
                     LAAVDVQNRVNQALGRLPNEVKNTGIIVTKQIGGFVFGAGVYSDTGQYDSLFLSNYMD
                     VYVKDAVKRVKGVGDAIIFGERKYAMRLWLDPAKLAQRGLTATNVLSALQEQNVQVAA
                     GQIGQPPSPEGQSYQISVRAVGRLSEASEFDKIILKTGTDGTLVRLKDVGHAELGAEN
                     YGTILRFNGHDAVGLAVTQLPGANALDVDKAAKAELLRLSKNFPPGIKAAVAFDTTTV
                     IGESIRDVLITLLQAIALVILVIYVFLQDWRSTFIPAITIPVSLIGAFVFVKLLGFSI
                     NTLTLFGITLATGLVVDDAIVVIENVERHIVEGITEPHNAASVAMKEVAGAVVATSLV
                     LVAVFVPVALFPGTTGILFRQFALTIAFSVAISAFNALTLTPALSAIFLGKHRERAKG
                     RFFKLFNDVFSAGARFYQSAVRRALDWRLASLLLFFALLGCTYWLYQRVPRAFIPEDD
                     QGYLMFIVQAPQGASLTYTRNVCGKAEEVISRDPEIDGVFTVVGFGAAGSASNQAILY
                     ANLRPFENRKGTVHSAAAAIQRLRGSLSAVSGAIVVPFNPPAVFGLGQFGGFQFELED
                     LGRNSLQSIAENANKLAAQGNASKDVV"
BASE COUNT         2499 a         3136 c         3603 g         2329 t
ORIGIN      
        1 gcatagatcg gccacggcat gatctggaga agacttccgt cgaacggatg cgggatacat
       61 ttcccgtcgg gggcacctgt gcaggtcgga tgcaccatgt ccgcatcgat gcccgtacgc
      121 atgatggcct tgaattgtgc aaaactgttc cctccttcag ctcttccggt cttgtcggga
      181 gtcaggttgc gcgaaataat gtgcggaaaa ggtcccggcc caggaaacgg accaaagtct
      241 ctaccgccgc ccagataggt cttgggattg accttcgtgg gtttttgacc aaagtaaggg
      301 tttcctccca ctacaaattc ggtggctggg cccttcgagt ggcagccatc gcagtcgccc
      361 tgtgcgttga caatatagct gcccaatccc accaagctcc ggttcttccc caccagatcg
      421 agcgggacag gtgcaatttc gaatcctcga cgaatctttg attcatttcc ctcgttgtca
      481 tcatcgtcgt cgtgggcttt cacacgtctc gaactgttca ccattcccgc gaaaacgatc
      541 gcaacaaatg ctgtgatcac tcccgcggct ttgaaaaact tactttttgt catggcaact
      601 ctcctccgtg cgttattttc cgaatatgct tcttgtcgtc ctgaactagc cgaatgtttt
      661 cgcgccttcg ctggatggca actggccatc agtaatgagg tttggctgga ctatatctag
      721 ttccttgcag gagtcaagga attaggtggt catgggtgga ccagtaccta agcaagcgcc
      781 cggatgagat acatcgcgat cagttgggaa gtcacattgg aacaagcaat tagttcttgc
      841 tgcggtcggc tgatacctga ttcgtggggt agtcggaaaa tgacggagca cccagcattg
      901 gtttcattgc gggggtttgc ggcgtctgcg ataatctggg gattgaatcc tgcaaaactg
      961 aggcggactt gacgggtaac gagacacgcg agaagtcctg gagtggcaac gacatccgta
     1021 aggcattcct ggacttcttc aagagtaagg ggcacacgat cgtgcggagc tcgtcgctgg
     1081 tgccgcatgg ggacgctacc ctgctgttta cgaatgcggg gatgaaccag ttcaaggacg
     1141 tgtttctggg gctggaaaag cgggagtaca cgcgggcgac gacggcgcag aagtgcgtgc
     1201 gcgcgggcgg gaagcacaac gacctggaaa atgtgggatt tacgaagcgg catcacacgt
     1261 ttttcgagat gctgggcaat ttttcgtttg gggattattt caagaaggat gcgattgctt
     1321 acgcttggga actgattact tcgccgcagt ggtttgggat tccgaaggac aagctttacg
     1381 tgacagtttt cgagcagtca gaaatatctc caggccaggt ctgcgagggc gaccacgatt
     1441 ccttattgta ttggagcaac acagacacag atctccccgc gactcgaatc cgcctgctgg
     1501 gcgcggaaac aaacttctgg gccatgggcg acacgggccc gtgcggtcct tgtagcgaaa
     1561 ttcattacga tatgggtcct gaagcgtccg acctcggcca caaagattgc ccgttctctt
     1621 gcgactgcgg ccgctacgtg gaaatctgga acttagtctt catgcagttc aatcgtgacg
     1681 cgagcgggac actcacgccg ttgccaaagc cctgcgtgga cacgggcatg ggcctcgaac
     1741 gcgtcgccgc cgtcctcgag cgcgtcatct ccaactatga aaccgacctc ttcacaccgc
     1801 ttgtcgacag ggctgaaaaa ctctgtagtt cgacgctaac gactcggcca gatctcgccg
     1861 catctttccg cataattgcg gaccactctc gcgccgccac atttttggtc tcagacggcg
     1921 tggttccttc aaacgaaggc cgcggctacg ttctacggaa aataattcgt cgtgcgttgc
     1981 gacactcgcg gattctgggg gcgccttcgt catttctatc caagatgtcg gacgccgtgc
     2041 gagcgctcat ggcgggtgcg tatccggaac tcgtggaaac tgcggatcgc gtaaaccata
     2101 ttctcgacga agaagaaacg cgtttcagtc gaacagtgaa tatagggctc aaaaaactcg
     2161 aggaagacct caccgagctg cgcgggccgg acctgataga gcacattgcg cgtgagccgc
     2221 tccgtggcac accttttaca gtcggacagt accctggaga taaggctttc aggctgtacg
     2281 atacatacgg tctgcctcgg gacttcatac aggatgtggt tcgtgacgcc accttctctg
     2341 ttgattggga gggtttcgac cgcgcaatgc aggagcaacg gacgcgggcg cgggcgtcgt
     2401 ggaagggtgg ggcgaaggaa gcggcgaatc cggcgtatgc gaagattgcg gaccgattta
     2461 cgacggagcc ggacttttat tttgggacga gcgcgaaaga ttgtggcatt gaagccatca
     2521 ttcttcctgg cggcgctcac cgcgccgaca cgaagagcgg gccggtgaat gaactgaagg
     2581 cgggggagag cggagaaatt gtgctcgacc gcacggcgat atacgcggaa tcgggcgggc
     2641 aggtggcgga caccggggcg ttctacgaca actcggaatc gcaactgctc gcggaagtga
     2701 cgggagcttt ttatcccgtt gctgggctgg tggcgcacaa agttgtggcg aaggagacgc
     2761 tgcgcgtcgg tgaccgcgtg gcggtggtgg cggatgcgga gcggcgagcg cggattatcc
     2821 gcaatcactc ggggacgcat ctggtgaatg cggcgctgcg gaatattctg gggacgcatg
     2881 tgaagcaggc ggggtcgctg aatgcgccgg agcggctgcg gtttgatttt tcgcactttg
     2941 cgcacgtgga tgcggaagag ctgcgggacg tcgaacagca ggtgaacgac gagatccggc
     3001 tgaacacgga gatcgagacg aacgtgactt cgctggaaga ggcgctggct tccggggcgc
     3061 tggcgttttt cggggacaag tatccggagc acaacgttcg cgtggtgacg attcccgatc
     3121 cgcgagcggc ccgggggttc tactcgaagg agctttgcgg cgggacgcac gtccagcgcg
     3181 ttggagacat cggcgtgctg aagatcgtga gcgaagaatc ggtcgcggcg ggcgttcgcc
     3241 gcatcgaagc tgttacgggc atcggggcgc tggagcattt tcaaaaccag gcggaaatcc
     3301 tgcgccaagt ggctagcaag ctgaacgtgg gggaggacgg catcctggcg caggttgaga
     3361 agctttccca gaccgccaag catctggaga aggagctgga ggcgcagaag cgcaaagggg
     3421 cgctcagcca gatcgacgag ctggcggcgc gggtgcaaat ggtcaagggg gtcaaagtga
     3481 tcgccgccga agtgcaaaat gttgaccgcg aaggacttag gcaactcgtg gacagcctgc
     3541 ggcagaaact gggctccgga gtggtggcgc tggggatgcc ggaagacgga aaagttgccc
     3601 ttattgcggg cgtcaccaag gacctgaccg ctaaagtcca tgctggcaag ctgatacagg
     3661 cactggccaa acgggtaggc ggttcaggtg gtggaagacc tgacctggcc gaggctggcg
     3721 gaaaagacat aactggccta aaaatggcct tggagagtgt cccctccctc cttgagcccc
     3781 tgttataatc agtggagcgc cagaagcgtg ctcggcggaa tacggtttca agagcgaccc
     3841 taataaccgg gtcgttacac gtgcgacccc taccgggtcg gcatccccgc caacacggct
     3901 tggcagaacg agccatcgca aggcgatctg cccctgggta aactgcccgg tcgagctcta
     3961 gtaccgatgg gacattgacc gctctgcggc aatgttgaaa actgtccgtt ctgtgatagt
     4021 tttcaagatt ggtgtttgcc ttccccaagg cacgacgccg gcccagccgg agtaaaggtc
     4081 gaatgataaa acgcacggca atcacggcaa ggcttgattt agcggcgctg gtcctttgcg
     4141 cggctttctt cgcaacattt ttcgcgcagg gcgccagcgg ccagatcgcc acggtcgttg
     4201 acgatggggg acggcggttt tttgtgaacg ccgagcggcc gttgacagcc aagctcactg
     4261 ccacgaagct gcgcaccaac atctacctgc ccgccgaatc ttccttttca ggacgcagcc
     4321 gtccggccat ggacatcggc cgcgacagcg tcgaaaaact cgttcgagaa gcggcggacc
     4381 ggcatcgcat ggaccctgca ctggtccgcg cggtgatcga aacggaatca aactggaatc
     4441 cgaaggctta ttcccacaag ggcgccggcg gattgatgca gttgattccc acgacggcac
     4501 aacgatatgg agcttgcgac gtatttaacc cgcaacagaa tatcgacgcc ggggttaagc
     4561 atctgaagtg gttgctagaa cgatacaatg gcaacctgga tttggcactg gcggcgtaca
     4621 atgcgggaga gggtgctgtt gaccgggctc atggcatccc cgcgtatcga gagacgcgga
     4681 actacgtaca gagagtacag aatgcctatt tccggccggg atcgggacga cttgcggacg
     4741 cattcatcaa ttcacacgcg atccacagag acctcacacc ggacggccgc atcatattca
     4801 cgaacgaata aagacagttg tcagtggtta gtgggtagtt ttcggggggc attgaactcc
     4861 ggacacaact gcttgaacta ttgctgaagt gagttgaggt ttccggttac gattgggtgg
     4921 catttggggg atgtaggttg atgttggggg ccaaaaaaaa tttgcacatg agcttcgccc
     4981 gcctttcgcc agtttttgcg gcgctgggtc ttgtgctgac ccttgcctct gctgcgccgg
     5041 cgaagacgaa tgcggaagct gcggccacgg cggcggtgag gcgacaatcc gcctccacac
     5101 aatttgcgcg ggccgaggaa cagcgcgcgg cgctgaacag caaacctacc gaaaagcgca
     5161 cgctggcgga ttacaaacag gtggtcaccg gctatcgccg cgtcgctttg attacgccgc
     5221 gagctcctga agtccccgat tcccttctgg cgatcgcgga actttacacc gagatgggcg
     5281 accggtttgg gcggagctat tatcagtcgg ccgccgattc ttaccaattc ctggtacacg
     5341 agtatccaac gagccggtat tgccaggatg cgatgctgcg agcggcaaag ctgcagcgcg
     5401 agcagctggg agattccgcg ctggccaaaa agacttacga ggatttcctg aaacgctatc
     5461 cgcgttcgcc gaggaaacgc gaagcgcaag aagctctggc ggaattggcg ctgttgcaga
     5521 acggcgatga gaccaacatc gtggcgcaaa attcaacggc ggcgtcacgt gccgcggagg
     5581 aaccgcgcgc gaatgcgccg gtacactccg gaggcaagtc cgtcagccgg tcggaatcat
     5641 cggaaattcc gcgagtgcgg cggatccgcg cggccgccac cggcgaggcg acgcgcgtga
     5701 ccatcgatct tgaagacagc gtgcaatacg cttcggggcg catcgcgaat cctgaccgca
     5761 tcttttttga tttgcacgcg gcgcggctga cgccggaagt cgctcgcggg aatatccgcg
     5821 ttgacggcgg gctgctgacc gccgtgcgtg tggcgcagaa tcatgacggc gtggtgcgcg
     5881 tggtgctgga cgtgaacgga gtaaaggatt acacggcttc gctgacaaat aatccggcgc
     5941 agcttttgat cgatttgtac ggaaattctc ccgcggcgcc ggtacagacc gccaaaggga
     6001 aaaagtcaca gcaggatgtg aaggaagatt cggtcgagag cgcagcgaac agttcgggcg
     6061 cggggagcaa ttcgcaaccg agcgtaaaaa acgccaaatc gaagtcagtc aaagccacgg
     6121 caacgggcgc gaagcccgat ttgatccggc cggcgagcgc gccgcagccg acgcgcgacg
     6181 ggcagtccac gctgacgcgc gcgctggggc tgaagattgg aaggatcgtg atcgacgcgg
     6241 ggcacggcgg ccacgacacg ggaacgattg gcccgacggg cttgatggag aaagacttgt
     6301 gcctggacgt ggcgctgcgg ctggggaaaa tcatcgagca gcggctgccg ggcgcggatg
     6361 tggtttacac gcgttcggac gacacctttg ttccgttgga agagcggacg aacattgcga
     6421 accaggccaa ggccgacctg tttatttcca ttcatgcgaa ttcgagcagg gatcacgcgg
     6481 cccgcggcat tgaaacgtac tacctgaacc tgaagggctc ggcggaagcg atggaggtgg
     6541 cagcgcgaga gaacgccacg gcgcagggag gcgtgcacga actgcaggat ctggtgatga
     6601 aaatcgcgag gacggaaaag attgatgagt ccaaggaact tgccgaggac atccaggatt
     6661 ccctcgccaa gcggattcaa aagagcagca agccggtaaa gaaccgtggc gtgcggaaag
     6721 ctccctttgt ggtgctgatc ggtgcggata tgccgtcgat cctgacggag atttccttcc
     6781 tgagtaatcc ggcggacgag caaatgctta agaagcccga gcaacggcaa aaagtggccg
     6841 aagggctgta tcagggcgtg gccagctacc tggagagcat gaacagcgtg acgatgaatt
     6901 tgccggtcaa aactggcgcc gggcaccccg ctgcggcggc cgttgaacaa tctcgcaacc
     6961 aaaactaggc agttggtgtc taatagaagc cgggatgcgc aagtattgcc tcatcttcct
     7021 gtcgtttttg gcggccgcca tggctgcctc gccccgtctg actgcgcagg gtgcactcga
     7081 gcgcgcgttc cccgagcgat ttgggcgatg gaaagctgtc aactccccaa tcaagatcaa
     7141 gagtgaggaa ggacaggcac aggtattcgc cgagtcaggt ttgacggact cgctcgctgg
     7201 tgagtattcc gacgggactc agaaagttgg agtcatactc cggcaattcg gggaccccag
     7261 cggggcgtat gaagcctaca ccgcagagtt cgggcctgac atgcatccgt ccacggtcgg
     7321 agggccttct gcgattgacc gggagcgcct gctgctgctc cttggcaacc tgattcttga
     7381 agtccggccg ccagaggatc caactactga tgaattgcaa cagcttgcgg cgatggtccg
     7441 gaaacattcc gacctgacgc cactgccgcc gattcgagcg ttcttgccgc cagggtttac
     7501 tgacgggacg cagagatatg cgcggggccc ggtggcgttc cggaatgcga ttgctttctt
     7561 gaagcaggac gaattcgcaa acctggtcag tgaagcgggc ttcaatgtaa acgcggaggc
     7621 aatgttcgcg cactaccggg caggaaaaaa tgaggcggtg ctgctgctga tcgaatatcc
     7681 tacgccgcaa ctggcggagc agcatcttcg gcatctggag caggcacttg gacccgcggc
     7741 gaagcaggag ggaacgacga ttgaacgcaa ggcttccctg ctctcgctgg tcttgaggcc
     7801 atcctcagcg gcttacggca acgcactgcg gagcgccgtg aattacgaaa cggaagtgac
     7861 gtggaacgag ccgcaccaga cggtcagcga tccgccattg ctcacaaccg tcgccaagat
     7921 ttttattagc acgggcgttt ttatggtggt gacggtggtc ctgggcgtgg cgtttggcgg
     7981 ggtgcgagtg ttggcgaaaa tctttttccc gggcaaggtt tttgaccggc cggaacagat
     8041 ggacgtccta caactcgggc tttctggaaa gagaatcaat tcgcgcgatt tctactagcc
     8101 cgctgctgga aacttccata taaccaacag ctttccccgg cgtccaacag gtgtcggact
     8161 ctttcgctat tttgctcttt cccatctgta tcgtaagatt tgaggtcagg taggcaggca
     8221 tgcacccatt tgtatcggat aatcgcagga cgagaagact aaccccggaa aggcgggggg
     8281 gcactttgct gctggcactg gcggcgggtg cttttgtgta cggctgccag aaaagcgcgg
     8341 cagacaatgc gccgggcgcc ggtgcgcctg ccatgacggt ccaagtacag attgctcctg
     8401 cggtgaaaat tccggacacc accgagtatc tgtccatcct aaagtcgcgg cactccgcag
     8461 cgatcaatcc gcaggttgag ggacagatta cacgaatttt cgtgaagtcc ggcgaccatg
     8521 tggaagcagg aacgccgctg ctgcagatcg acccgctgaa acaggaagcc acggtaaaca
     8581 gccaggaagc atcgcgcgcc gcccaggaag caaacctgcg ctacgccaag gtttcgttgg
     8641 agagggcgca gaagctgttt gacgccggag tgatcagcaa gcaggagctc gatggcgcac
     8701 agaccggcta tgacgcgtct gtcgcgcagg taaagtcgat cgacgaacaa gtaaggcagc
     8761 aaaaggtcga actccactac tacagcgtca cctcaccgat ggacggtatc gtcggagata
     8821 tcccggtgcg gatgggcgac cgcgtgacgg tttcgacgat gctaaccacc attgacgagc
     8881 ccggggcgct ggaagcttac atatacgttc ccgccgagcg tgccaagaac ttgaaagccg
     8941 gattacccgt gcggctgttg gacaccgcgg gcaattcggt caatgagact cgcattactt
     9001 ttgtgtcacc gcaggtcgag acggaaacac agacggttct cgccaaggct tcgattgaga
     9061 attcgcacag caaactgcgc gtcgcccagc aagtgcgcgc acagattacc tggggcgttc
     9121 acgagggtcc cgtgattccc gtgcttgccg tactccgcat caatggccag ttcttcgcgt
     9181 tcgtttccgt caacgaaggc aaaggaaccg tggcgaggca gaaaccttta aagcttggcg
     9241 atactgccgg caatgatttt gtggttctcg atgggctcaa acccggagat cacttgatca
     9301 tttccgggac gcaatttttg caagacggca tgccggtcac agaacaaatt cagagcaacg
     9361 gcaagggcgt cgcaggccag taggccactc aaccccatga gcggcaccac tccattccta
     9421 gtgaaagcgg actccggcct tgttcgttga tttcttcatt cggcggccgg tttttgccac
     9481 ggtctgcgcg ctattcatca ttcttgcagg cgctatctcc attcccacgc ttccgatcgc
     9541 agaattaccc gacctggcgc cgccgcaagt catcgtcagc agcggctaca ttggagcgaa
     9601 cgcccagacg gtggaatccg cggtcaccat tccgttggaa caagccatca acggcgtgca
     9661 gggcatgaag tacatcagct cgacgagcgg caacgacgga tcgagccaga tcacggtggt
     9721 attcgacgta acccgaaacg tcgacctggc agcggtggac gtgcagaacc gggtgaacca
     9781 agccctgggg cggctgccga atgaagttaa gaacacggga atcatcgtca cgaagcagat
     9841 cggcggattt gtgtttggcg cgggcgtcta ttccgatact ggacaatacg acagcctgtt
     9901 tctcagcaat tacatggatg tgtacgtcaa ggatgcggtc aagcgcgtaa aaggagttgg
     9961 cgacgcgatc attttcggcg agcgcaaata tgccatgcgc ctgtggctcg atccggcgaa
    10021 attggcgcag cgcggattga cggcgacaaa tgtgcttagc gcccttcagg aacagaacgt
    10081 gcaagtcgcg gccggacaaa tcgggcagcc gccttcgccg gaagggcagt cttaccagat
    10141 cagcgtgcgg gcggttggtc gcctcagcga agcatcggag ttcgataaaa tcatattgaa
    10201 gacaggaacc gacgggacgc tcgttcgatt gaaagatgtc ggccacgcgg agctgggcgc
    10261 ggagaattac ggcaccatcc ttcgattcaa cggccatgac gccgtcggcc ttgcggtcac
    10321 ccaacttccc ggcgccaatg cgctggatgt ggacaaggcc gcaaaggcgg agctgttgcg
    10381 gctctcgaag aattttcctc cggggatcaa ggcggccgtg gccttcgata ccacgacggt
    10441 catcggcgaa tccatccgcg acgtactcat cacgctgttg caagccatcg ccctggtcat
    10501 cctggtcatc tacgtgttct tgcaggactg gcgaagtacg tttatccccg cgattaccat
    10561 ccccgtttcg ctgatcggcg cgttcgtttt tgtaaaattg ctgggtttct cgatcaatac
    10621 gctgacgctc tttgggatca ccctggccac cgggctggtc gtggatgacg caatcgtagt
    10681 gattgagaat gtggagcggc acatcgtcga gggcatcacg gagccgcaca acgccgcatc
    10741 cgtggcgatg aaggaagtgg cgggagcggt cgtagcaaca tcactcgttc tggtggcggt
    10801 attcgtgccg gtcgcgctat tccccggaac caccggaatt ctcttccggc aattcgcgtt
    10861 gacgatcgct ttttccgtcg cgatctcggc cttcaacgcg ctcaccctga ccccggcgct
    10921 ttcggcaatt ttcctcggga aacatcgaga gcgcgcgaag ggccgtttct tcaaattatt
    10981 caatgatgtc tttagcgccg gcgcaaggtt ttatcaaagt gcagtccgcc gggccctgga
    11041 ctggcggctg gcctccctgc tgctcttttt tgccctgctc ggctgcacgt actggctcta
    11101 tcagcgcgtt ccgcgcgcct tcatccccga ggacgatcag ggctacctga tgttcatcgt
    11161 gcaagccccg caaggcgctt cgctcacgta cacgcgcaat gtttgcggca aggcggagga
    11221 agtgatctcg cgcgatccgg aaatcgatgg tgttttcacg gtcgtcggat ttggcgccgc
    11281 gggctcggcc tccaatcagg caattcttta tgcaaatctg cggccttttg agaatcgcaa
    11341 gggaactgtt cactcggccg ccgcagcgat tcagcgcttg cgcggttctc tctcggcggt
    11401 atcaggcgcg atcgtagttc cgttcaatcc accggcggtg tttggactgg ggcagttcgg
    11461 cggattccag tttgagctgg aggatctggg gcgcaactcg ttgcagagta tcgcggaaaa
    11521 tgcgaacaag ctggccgcgc aagggaatgc tagcaaggat gtcgtgg
//
LOCUS       DASYVO010000229         3706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_215280, whole genome shotgun sequence.
ACCESSION   DASYVO010000229 DASYVO010000000
VERSION     DASYVO010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215280"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..580)
                     /locus_tag="VGR03_07615"
     CDS             complement(197..580)
                     /locus_tag="VGR03_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994181.1"
                     /translation="MSVRISPDANRPSATIEIPLENPLPDYDLLQLEQPTPRDVDAVL
                     VSQGFRNLVDDARGVLTELLNDTSLELAQFTGAICPGDDESYRPGLWIVLRDKNSVQG
                     RELSSDSRTRISAIANELVKRLQLA"
     gene            complement(607..1035)
                     /locus_tag="VGR03_07620"
     CDS             complement(607..1035)
                     /locus_tag="VGR03_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV1994182.1"
                     /translation="MRLRLLMLGKTRRPEMRAVLDDYVKRIGHSCPAEITEVRDAPAA
                     LKKLDADRAATVVLLDAAGKNLDSNALAKWLGDLRDRGTRELIFLCGDADGFPDDLRQ
                     RAHQKLSLSSMTFSHELARVMLVEQLYRAFAILSGSPYPK"
     gene            1241..1477
                     /locus_tag="VGR03_07625"
     CDS             1241..1477
                     /locus_tag="VGR03_07625"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEV1994183.1"
                     /translation="MSAPARAIHNSAIVVDTHEDTPQRFLDENYDIGSIDPQDHDFIS
                     LDKARAGNLGAEFFSIFVDPKRTRDTSVNKWYWK"
     gene            1489..2112
                     /locus_tag="VGR03_07630"
     CDS             1489..2112
                     /locus_tag="VGR03_07630"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEV1994184.1"
                     /translation="MMVDISHVSDKTFWDVIATTKSPVIASHSSARALRDTPRNLTDE
                     MLRAVARNGGVVNVAFANMFLDQEFGKAALSQHEGMLSALEAYNAKRQDAGQPVTPED
                     SQRFNLEWLDRNKIPRAALKSLIDHIDHVTKVAGVDHVGLGSDFNGADFFPEGIDSAA
                     DLPKITQALLELGYTPDDIRKILGGNMLRVFRDVERVSREMQTRHLP"
     gene            complement(2121..3074)
                     /locus_tag="VGR03_07635"
     CDS             complement(2121..3074)
                     /locus_tag="VGR03_07635"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV1994185.1"
                     /translation="MPWISADPASLRLFELAEKVAVAPATLLITGESGTGKDHLARLV
                     HELGPRRDAPFLKIDCASLPPQLVESELFGHERGAFTGAVERKLGRFELGGNGTIVLD
                     EVAALSPGAQGKLLRILQERTFERLGGTETLRIEARLIALTNVDLPAAVKAGSFREDL
                     YFRLNVLTLEVPPLRERRADILPLADHLLATLRSVHGRPNAQLSDPARRMLAAYAWPG
                     NVRELRNALERAVVFSRPSRGTAATKSDLLEPENFPENVRAAAPGAASQVAGLRSLEE
                     VEREVIAATLEATHYQISRSAEALGISRKTLLEKRKKYGLK"
     gene            complement(3086..3553)
                     /gene="rsfS"
                     /locus_tag="VGR03_07640"
     CDS             complement(3086..3553)
                     /gene="rsfS"
                     /locus_tag="VGR03_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV1994186.1"
                     /translation="MKFESLPQGVRLAVEAAQEKKAAGITVLDLTAAGAFTDYFVVCT
                     GFSTPQVQAICTEVEAQLYKNLRRSPEHREGHRSAEWALLDFGGFVVHVFSEQARRYY
                     DLERLWRMSPKLELPDQPDGLSSERNMPDSRFGHEHFSGEAVSSRPQSGRSRQ"
BASE COUNT          753 a          974 c         1228 g          751 t
ORIGIN      
        1 tgcctagcgc gcaggtggtc ttccccttgc ccggcccggt gtaaacgatg agcagtcctt
       61 tttcttccgg catggtctta cgattcggtg aaccctttca aagggcggcg aaaagactag
      121 agtttgcgga gtcgtaagca aaaggtcaag ttcgcgcggg aggcacttcc ttgccagtgg
      181 cttgtgcgcg tggcaatcac gcgagctgga ggcgtttgac gagttcgttg gcgatggcgg
      241 agatgcgcgt tcgtgaatcg gacgagagtt cgcggccctg gacggaattt ttgtcgcgga
      301 ggacgatcca gagtccgggg cgataggatt cgtcatcgcc ggggcagatg gcgccggtaa
      361 actgggccag ttcaaggctc gtgtcgttca gcagttcggt gaggacaccg cgggcgtcgt
      421 caacaaggtt gcggaaaccc tgcgagacga ggacagcgtc cacatcgcgg ggcgtcggct
      481 gttcgagttg gagaaggtcg taatcgggga gaggattttc gagagggatt tcgatggtag
      541 cggagggccg atttgcgtcg ggagagattc gtacggacat gatgctccta ttttgcacca
      601 cgcgggctat ttcgggtagg ggctgccgga gagaatggcg aaggcgcggt aaagctgctc
      661 gacgagcatg acgcgggcga gttcgtggga gaaggtcatg gagctgagag aaagtttctg
      721 gtgggcgcgc tggcggaggt catccggaaa gccatcggcg tcgccgcaga ggaagatgag
      781 ttcgcgcgtg ccgcggtcgc gaaggtcgcc gagccatttg gcgagggcgt tcgaatcgag
      841 attctttccg gcagcgtcga gaaggacgac ggtggcggcg cgatcggcat caagtttctt
      901 gagggcggca ggggcgtcgc ggacctcggt gatttccgca ggacacgagt gaccgatgcg
      961 ctttacataa tcgtcgagaa cggcgcgcat ctcggggcgt cgcgtcttac cgagcatgag
     1021 cagtcgcagg cgcatgggag tactctagcg caactacgtg ccgcggcccg catagcacca
     1081 atgcctggta gtagactggt ttgcctcggg atccaaaact gattcgaggg agcaccatag
     1141 cgattacaat aggggcacaa tgagacttgc gagaaacttg gtttacttgg ttcttctcat
     1201 tggaccgttg gtttggtctg tcttcgccca agaccaccct gtgagcgccc ccgctcgcgc
     1261 catccacaat tccgcaatcg ttgtggacac gcacgaagac acgccgcaac gctttctcga
     1321 cgaaaactac gacatcggct ccatcgatcc acaagaccat gattttatta gcctcgacaa
     1381 agcccgggct ggtaacttgg gtgcggaatt cttctctatt tttgttgacc caaaacgaac
     1441 cagggacact tcggtaaaca agtggtactg gaaatgaacc ggctcggaat gatggtggac
     1501 atctcccacg tttccgacaa gactttctgg gatgtgatcg caacgaccaa gtcgccggtg
     1561 atcgcttctc actcttccgc acgagctctg cgcgatacgc cccgcaattt gacagacgaa
     1621 atgttgcgcg ccgtcgcgag gaatggcggc gttgtaaacg tggcctttgc caatatgttc
     1681 ctggaccagg agtttggtaa ggctgctttg tcgcaacacg aaggtatgtt gagcgccctc
     1741 gaagcttaca acgctaaacg gcaggacgct ggccagcctg ttactcctga ggatagccag
     1801 cgctttaatc tggaatggtt ggacagaaac aagattccgc gggcagcact aaagtcgttg
     1861 attgatcaca tcgatcatgt gacgaaagtc gccggtgtgg atcatgtcgg cctcgggtcg
     1921 gatttcaacg gggccgactt cttccctgag gggatcgatt ctgccgccga cctcccgaaa
     1981 attacgcagg cgcttttgga gcttggatat acacccgacg acatcaggaa aatcttgggc
     2041 ggcaacatgc tccgagtgtt tcgcgatgtt gaacgagtta gtcgggagat gcagacgcgc
     2101 catttacctt agacgcccca ttacttcagg ccgtacttct tgcgcttttc gaggagggtc
     2161 ttacggctga tgccgagagc ttcggcggag cggctgatct ggtaatgcgt agcttcgagg
     2221 gtggcggcga tgacttcgcg ctccacttcc tcgagggaac ggagcccggc gacctggctg
     2281 gcagcgccag gcgcggcagc tcgcacattt tccggaaaat tttcgggctc gaggaggtca
     2341 ctcttagtag ccgcagtgcc gcggctgggg cgcgagaaaa ccaccgcgcg ctcgagggcg
     2401 ttgcggagtt cgcggacatt gccaggccag gcgtaggcag cgagcatgcg gcgcgccgga
     2461 tcgctcaact gcgcattggg gcggccgtgg acggagcgaa gggtcgcaag aagatgatcg
     2521 gcaagaggca ggatgtcggc gcggcgctca cgaagcggcg gaacttcgag tgtgagaacg
     2581 ttcagccgaa aatagagatc ttcgcggaag ctgccggctt tcacggcggc gggaagatcg
     2641 acgttggtga gggcgataag gcgcgcttcg atgcgcaggg tttccgtgcc gccgaggcgt
     2701 tcgaacgtgc gctcctgcaa gatgcgcaag agcttgcctt gcgctccggg tgaaagcgcg
     2761 gcgacttcat cgagaacgat ggtgccgtta ccgccgagct cgaagcggcc gagtttgcgc
     2821 tcgaccgcgc cggtaaaagc gccacgttcg tggccgaaga gctcgctttc aacgagctgt
     2881 ggcggaaggc tcgcgcaatc gattttcagg aacggcgcgt cgcggcgcgg gccgagttca
     2941 tgaaccaagc gcgcgagatg atccttaccg gtcccgcttt caccagtgat caagagcgtg
     3001 gctggagcga ctgcgacttt ctcggcgagt tcaaaaagac ggagcgatgc gggatccgcg
     3061 gagatccatg gcaatcgctc ctcggtcatt gccgcgaacg accgctctgc ggccggctgg
     3121 aaacggcttc gccggaaaaa tgttcgtgcc cgaagcgcga atcaggcatg tttcgttcgg
     3181 aagaaagacc gtcaggctgg tcggggagtt cgagcttggg cgacatgcgc cagaggcgct
     3241 cgagatcgta atagcgccgg gcttgctcgc tgaaaacgtg cacgacaaag ccgccgaaat
     3301 cgagcagcgc ccattcggcg gagcgatgtc cctcgcgatg ttcgggcgag cggcggagat
     3361 ttttgtagag ctgcgcttcg acttcggtgc agatggcctg cacctgcggg gtgctgaagc
     3421 ccgtgcaaac cacaaagtaa tcagtgaagg cgcctgctgc cgtgaggtcg agcacagtga
     3481 tgcccgcggc tttcttttct tgtgcggctt cgacggcgag gcggacgccc tggggcaagg
     3541 attcgaattt caccggtaaa gttcctgttt gagaatgtat tcctcgacgc gtgcggaaac
     3601 aagtccgtgg atggactggc cgcgttgcgc gcggcgccga acatccgtcg cggaaacatc
     3661 gctggagaca ttttcgagca gataaacggt ggtcttgtga aggtgc
//
LOCUS       DASYVO010000230         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_175834, whole genome shotgun sequence.
ACCESSION   DASYVO010000230 DASYVO010000000
VERSION     DASYVO010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175834"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VGR03_07645"
     CDS             <1..210
                     /locus_tag="VGR03_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994187.1"
                     /translation="GAVAAGLPGARAKFFATPQEAAEFLAGFIGDGDLLLVKGSRGVK
                     MEQIVESLIARHAAPGEITGQEVRH"
     gene            212..1357
                     /gene="mraY"
                     /locus_tag="VGR03_07650"
     CDS             212..1357
                     /gene="mraY"
                     /locus_tag="VGR03_07650"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV1994188.1"
                     /translation="MLYYLLYHTLQRYFSPLNVFRYITVRTVYASLTAMFLALVFGPW
                     LIRRLRELQIGQFIREEGPEEHKKKAGTPTMGGVLIVLSTAVPVLLWADLTNPFILLS
                     LFALFGFAVIGFIDDYAKVSKQRNLGLTAKKKIYFQVFVSLIVGVVLLVLATYSAYST
                     QFVFPFLKRFHPDLVIHSLLDQSHFSGHLWPLAFLPFLVATVIVIVGSSNAVNLTDGL
                     DGLAIGCTVIAAGALTVLTYVSSNYRWATYLEIQYIPRVGELTVFCGALVGASLGFLW
                     YNAHPAEIFMGDVGSLSLGGTLGTVAVIIKQEILLFFVGGVFVIEALSVILQVGSFKL
                     RGKRIFRMAPIHHHFELLGWSESKVIVRFWIAALVFALFALTTLKLR"
     gene            1393..>2374
                     /gene="murD"
                     /locus_tag="VGR03_07655"
     CDS             1393..>2374
                     /gene="murD"
                     /locus_tag="VGR03_07655"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV1994189.1"
                     /translation="MPNPFELRGKRVLVVGLARTGVATALFCAARDANVTATDARTEN
                     EIGEAIAPLRTAGVSLELGGHRENSFLEQDLIIPSPGVPANVPLLQSARAQGVTIWSE
                     VELADRFLDGRLIGITGSNGKTTTTSLIEHILKNAGFSTLLAGNIGTPLIARVEQTSD
                     DTITVVELSSFQLELIESFRPNISVFLNLTPDHLDRHHTLEEYGRAKARIFENQTEAD
                     SAVLNADDSATTPLAPTKPHVYWFSRKQRVAQGAFVRENEIIFRHDGEEEAVMNLQEI
                     PLAGAHNVENVLAAVAATRLAGAEPAAIAKGVRSFAGVEHRLEFVAEIGGV"
BASE COUNT          433 a          659 c          723 g          559 t
ORIGIN      
        1 ggcgccgtgg ctgccgggct cccgggcgca cgcgccaaat ttttcgcaac gccgcaggag
       61 gctgccgaat ttctggcggg gtttatcggg gacggcgatc tgctgctggt gaaaggctcg
      121 cggggcgtga agatggagca gattgtggaa tcgctgatcg cgcggcacgc tgcgcccggt
      181 gaaattacag gccaggaggt tcggcactga aatgctttac tacctccttt accacacgct
      241 gcagcgctac ttcagcccgc tgaatgtctt tcgttacatc accgtgcgga cggtgtacgc
      301 gagtctcacg gccatgtttc tggcgctggt ctttggtccc tggctcatcc ggcgtcttcg
      361 tgaattgcag atcggccagt tcattcgcga ggagggccct gaggagcaca agaagaaagc
      421 gggcacccca accatgggcg gagtgctcat cgttctttcc acggcagttc cggttctcct
      481 ttgggcggat ctgaccaatc catttatcct gctctcgcta ttcgcgctct tcgggttcgc
      541 cgtcattggc ttcatcgatg actacgcgaa agtctcgaag cagcgcaatc tgggtctcac
      601 cgcgaagaag aagatttatt ttcaggtctt cgtcagcctg atcgtcggcg tcgtgcttct
      661 tgtgttggct acctacagtg cgtactcgac gcaattcgtc tttccattct tgaaacgatt
      721 ccatcccgat ctggtgattc actcgttgct cgatcaatct catttttccg ggcacctctg
      781 gccgcttgcg tttttgccct ttcttgtggc cacggtaatc gttattgtgg gttcctccaa
      841 cgcggtgaac ctgacggatg ggctggacgg cctggcaatc ggctgcacgg tcatcgccgc
      901 gggggcgctt acggttttga cctacgtgag cagcaactac cgctgggcca cctatctcga
      961 gattcagtac attccgcgcg tcggcgagct cacggtattc tgcggtgcgc tcgtgggcgc
     1021 ttcgctcgga tttctctggt acaacgcgca tcccgcggaa atattcatgg gcgatgttgg
     1081 ctcgttgtcg ctgggcggaa cgttgggcac cgtcgccgtc attatcaagc aggaaatttt
     1141 gctttttttc gtcggcggtg tatttgtcat cgaagcgctt tccgtgatcc tgcaggtggg
     1201 ctcgttcaag ctacgcggca aacgcatctt ccgtatggcg cccatccatc accatttcga
     1261 attgctcggt tggagcgaat cgaaagtaat cgtgcggttt tggatcgccg cgctggtttt
     1321 tgctttgttt gcgttgacga cgttgaagtt gaggtagcgg agcacttcgt tcaaagtgtt
     1381 ttacggacaa agatgccgaa tccgtttgag ttgcggggca agcgcgtgct ggtcgtggga
     1441 ctggccagga ccggtgtggc caccgcgctc ttttgcgccg cgcgggatgc gaacgtgacg
     1501 gcgacggatg cgcggacgga aaacgaaatc ggcgaagcga ttgcgccact ccggactgcg
     1561 ggcgtaagcc tggagctggg cggccatcgc gaaaattcat ttctcgaaca ggacttgatt
     1621 attccgagtc ctggcgtgcc cgccaatgtt cctttgctgc aatccgcgcg cgcacagggt
     1681 gtcaccatct ggagcgaggt ggagctggcg gaccgttttc tcgacgggcg cttgatcggg
     1741 atcactggtt cgaacgggaa gacgacgacg acttccttaa tcgaacacat tttgaagaac
     1801 gctggttttt ccacgcttct cgccgggaat atcggcactc ccctgattgc gcgcgtggag
     1861 cagaccagcg acgataccat cactgtggtg gagctaagca gttttcagct tgagttgatt
     1921 gaatcgtttc gccccaacat cagcgtgttc ctgaacctca caccagacca tctggaccgg
     1981 caccacacgc tcgaagagta tggccgcgca aaagcgcgca tcttcgagaa ccagacggaa
     2041 gcagacagcg cggtgttgaa cgcggatgat tcggcgacca cgccactggc gccaacaaag
     2101 ccccacgtgt actggttcag ccggaagcag cgcgtggccc aaggtgcgtt cgtgcgggaa
     2161 aacgagatta tctttcgcca tgacggcgaa gaagaggcgg taatgaatct ccaggagatt
     2221 ccgctggcgg gtgctcacaa cgtcgaaaac gttcttgcgg cggtggcggc gacgcgtctt
     2281 gctggggccg agcccgcggc tatcgcgaaa ggggtgcgct cgttcgcggg cgtcgagcat
     2341 cgcctcgagt ttgtcgccga aatcggaggc gtgc
//
LOCUS       DASYVO010000231         7828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_175852, whole genome shotgun sequence.
ACCESSION   DASYVO010000231 DASYVO010000000
VERSION     DASYVO010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7828
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175852"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(560..1228)
                     /locus_tag="VGR03_07660"
     CDS             complement(560..1228)
                     /locus_tag="VGR03_07660"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV1994190.1"
                     /translation="MRREKTSVSQEDYLKAIWEMLEEDQMPISARLAEELKVTPPAVT
                     AALKRMTRDGHVRVERSGRIDLTQKGRKRAEHLALRHQLAEMLLTEVIGLSWAKAHDE
                     AERLEHAISPEVEALLLKRFGDKKSCPHGVPMRGGIAQLRKQGAVLLADLRAKEAGEI
                     VCVYEKDAQFLEFLEGLRLRPSTTVEVLRREYDETMTLRSGGKTMHLGKPATTRIWVR
                     RISS"
     gene            complement(1237..1704)
                     /locus_tag="VGR03_07665"
     CDS             complement(1237..1704)
                     /locus_tag="VGR03_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HEV1994191.1"
                     /translation="MKKIRKENPAARRSSRTRVLFVCVGNACRSPMAEAIARYDSADV
                     IEASSAGLSPMGSIAEQTKQTLILNGYSADGLTSNSLTREAADAADIIINMTGKPGEE
                     TFWDQEKVEDWMVQDPYGAVPETYQRVFEGIKRRVNQLALGLREKRENKTRAK"
     gene            1953..3188
                     /locus_tag="VGR03_07670"
     CDS             1953..3188
                     /locus_tag="VGR03_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEV1994192.1"
                     /translation="MSFRAVFIAIVIAFALILSAFLVQRARPRIETDQPNASFVKATG
                     KCAECHAHQQYSVVHEYEMSKHAAKSVTCLDCHQPAQGQEKKEHNGFVITTHMTAGNC
                     RPCHEQVYQQFVRSRHAAPSWAAVMGEKGLTVEQADFAEKYNPGYVRRPANALAVLEG
                     TSAISGGCGQCHAIGKPNEDGTIGNCTACHTRHTSSVAIARRPSTCGQCHMGPDHSQI
                     EIYEESRHGVLFAAQERQLNLDADPKSLTTRDMFVPTCATCHMSGINGLKVTHDPGER
                     LSYYLADAISKQRPNYLLAQANMKQICNQCHSKTLIDRVYNQAEDVVKSTNEKVQRAQ
                     ELVAGLRKEGSLGSQPFQKPIDFLLFDLWHYDGRTSKHGAFMGGADFVQWHGNYELLK
                     KQAQLEAMAEELRAKHGHK"
     gene            3175..4011
                     /locus_tag="VGR03_07675"
     CDS             3175..4011
                     /locus_tag="VGR03_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994193.1"
                     /translation="MATSEVPVNGQSEWVGAEDLWLEVFVIFNFVCLTGDIAIAHAAN
                     HFRNAAEYIPLWFSLLAAAVLVVGIAGRLRNNRQSVWRDLGYLVGWMSIAVGAAGVVY
                     HLNSSFFYERTLKSLTYAAPFAAPVAYMGLGCLLLMNRMVARHTREWAQWVLFFTLGG
                     FVGNFVLSLSDHAVNGFFRWSEWIPVISSGFAVGFLLALLLLDERRGFLWLTAGILFV
                     QILVGGLGFLLHAWADWHGPAGSLFQNIVSGAPPFAPLLLPNLSLLGFIGILAMDRAI
                     PK"
     gene            complement(4042..4635)
                     /gene="pdxT"
                     /locus_tag="VGR03_07680"
     CDS             complement(4042..4635)
                     /gene="pdxT"
                     /locus_tag="VGR03_07680"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV1994194.1"
                     /translation="MKIGILAVQGDFEAHAAMLARLGAETAEVRTPAQLEGCDGLILP
                     GGESTTQLQFLQEEGLADAIAKFAKQGGAILGTCAGAILLATEVQNPKQDSLKLLDMT
                     VLRNGYGRQIASDVVSGPTKLKSEPLEMVFIRGPIIERAGPGVKVLAEYAGKPALVQR
                     DNIMAATFHPELTDDTTIHRHFLELAAASSRQTTAAK"
     gene            complement(4716..5636)
                     /gene="pdxS"
                     /locus_tag="VGR03_07685"
     CDS             complement(4716..5636)
                     /gene="pdxS"
                     /locus_tag="VGR03_07685"
                     /inference="COORDINATES: protein motif:HMM:NF003215.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV1994195.1"
                     /translation="MDAKKVNGSGNGSKGSNEWRVKVGLAEMLKGGVIMDVTNAEQAK
                     IAEKAGACAVMALERVPSDIRKEGGVARMASIKVIREIMATVTIPVMAKVRIGHFAEA
                     DVLQALEVDFIDESEVLTPADEENHIWKHDYRVPFVCGARNLGEALRRIAEGAAMIRT
                     KGEAGSGNVVEAVRHMRAIVSQMKKLTTMRDDELMREAKELGAPLELIRQIAKTGKLP
                     VPNFSAGGIATPADAALVRRLGAEAVFVGSGIFKSSDPETRAKAIVKAATHFEDPKAV
                     LEAHEELGEAMPGLDIRQMEEKDMLSTRGW"
     gene            complement(5694..6116)
                     /locus_tag="VGR03_07690"
     CDS             complement(5694..6116)
                     /locus_tag="VGR03_07690"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV1994196.1"
                     /translation="MSETITLAPNPTNKCFGCGGDNAGGMKLTFEQDNVNRRIVGRFV
                     LGERYQGGGGFAHGGIIATLLDEAMGKVCRFREVRAVTAEMTVEYLKPVDVMKEIEVE
                     GRETEQKGRNIFMTGEIRDTDGIVLARGRARFVVIGQK"
     gene            complement(6185..7729)
                     /locus_tag="VGR03_07695"
     CDS             complement(6185..7729)
                     /locus_tag="VGR03_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV1994197.1"
                     /translation="MFPLHLQPQSHVPLYIQLRDQLRALVHAGDLRPGDRIPASRELA
                     TMLGVHRTTVANAYAELESEGLIQGHVGRGTFIRANGNGLKITPPAPPVLNGNGGIRW
                     ELLFADERGEEVLSRLTANVPEDALSFVMARPAQEFFPIEELQTCVNAVLRREAAEVL
                     NLGPSDGYPPLKAALLEMLHHDGIAAKDENLLITDGCQQSFDLISKAFVRPGDSVIME
                     NPTYPGAVAIFHGARARCLSVPMNTHAEPGTPLGLDLEALEATLAANRVKLIVLTPDF
                     QNPTGTSMPLASRRKVLELAGRHQVPVVEDHIYARLHAREERIPSLKQLDRSNLVIHI
                     DSFAKVAFPGLRVGWIVAPATAIERLRVVKQTTDLHTDQLAQATLAEFLRRGLFSKHL
                     AKMRKVYGSRLAALDEALRKHMPEGTRWTKPEGGMCLWLELPLGFDASELLIHVKERG
                     VLFAPGRYFYVQTPQPNTLRLGFASLDEKQIARGVATLAELLKIEMRKRQRGVRRAER
                     SRVALV"
BASE COUNT         1494 a         2521 c         2042 g         1771 t
ORIGIN      
        1 cgcgtggtca cggctcccta ctccttgacc tataatactt ccgggccgtc ggcaactcga
       61 aactaatcgc ctaagtcctg attaacgcgc tcaccggaca agtgaaactc cgccaagttt
      121 tcagaagccc cgaccgggga aacgaccaat tcacctcaaa ttgacctgcc cttggaaccc
      181 cggttcgccc ttgatttcac ttgcggccct ggtataatac agccagggaa aaccgggttt
      241 cctccctcgc gttccgcttt tttcgacgtt tctatttgca ccaaattgca gcaaaaagcc
      301 tgtaagccct ttctttgcca cacatccata aaatgtgccc ataactcctt tatttgccac
      361 acatccgaaa aaagtggggg gtgggagtga ttttgttaac tagaaatcct gggagggatt
      421 tctgccccga atccgccgcg gctgatgagc aaagtgtctc tcttcctggc ctgcgcacca
      481 aaaagattta gggcgagagc ttcgacgacc gagggcgcaa ggattaagtg ccggtcaaga
      541 atacgaagca acccctcctt tacgaactga ttcgccgcac ccaaatgcgg gttgtggcag
      601 gcttgccgag atgcatcgtc ttcccgccgg aacgcagcgt catcgtttca tcgtactcgc
      661 gcctcagcac ctctacggtc gtcgatggcc gcaagcgcag cccctcgagg aattccagaa
      721 actgtgcgtc cttctcgtaa acgcaaacaa tctcgcccgc ttccttcgca cgcaaatcgg
      781 cgagcagcac cgcgccctgc tttcgaagct gcgcgattcc tccgcgcatg ggcacgccgt
      841 gcggacagct tttcttgtct ccgaatcgct tgagcaacag cgcttccacc tccggagaaa
      901 tcgcgtgttc cagccgctcc gcttcgtcgt gagccttcgc ccaggacaac ccgatcactt
      961 ccgtcagcag catctccgcg agctgatgcc tcaatgcgag atgttccgca cgcttgcggc
     1021 ctttttgcgt tagatcgatc cgcccgctgc gctccacgcg cacgtgaccg tcgcgcgtca
     1081 tgcgcttcaa agcagcagtc acggcgggag gcgtaacttt caattcctcg gcgagccgcg
     1141 cgctaatggg catctggtcc tcttccagca tctcccagat ggccttcaga tagtcttcct
     1201 gcgatacgct tgttttttct cgccgcaacg ccactcctat tttgcccgtg ttttgttctc
     1261 tcgtttctct ctcagaccca gagccagctg gtttacgcgc cgcttgatgc cttcgaatac
     1321 cctctgatac gtctccggca cggcgccgta aggatcttgc accatccaat cctcgacttt
     1381 ctcctgatcc caaaacgttt cctctccagg cttgccggtc atgttaatga tgatgtccgc
     1441 ggcatcggcg gcttcgcggg tgagcgaatt ggaagtcagc ccgtctgcgg agtagccatt
     1501 cagaatcagc gtttgcttgg tttgctcggc gatggatccc atcggactca aacccgcgct
     1561 ggacgcttcg atcacgtctg cggagtcata gcgggcgatg gcttcagcca tgggactcct
     1621 gcacgcgttc ccgacgcaca caaagagaac cctcgtccgg cttgaccttc gagcagcagg
     1681 attctctttc cttatttttt tcacggtgag actttcgcgc tagcctggtt cctcacttgt
     1741 taccggcgcc caccatcaaa gcgtagctgg gattcccttc gacggcaacc caaaggcagc
     1801 aattccctgg tggatacaat cgaattgcgc caccatagct cgcgctggcg gcaaggaatt
     1861 ccctatgttg cgtacgccct ttgtgatatt tcttgcttcc ccgagcgagc gaatctcgca
     1921 tcagcgagcc ccaatgagct ctggaggagt acatgtcctt tcgcgctgtg ttcattgcca
     1981 tcgtcattgc gtttgccttg atcctgagcg cgtttctcgt tcaacgcgcg cgcccgcgca
     2041 tcgaaactga ccagcccaat gcctctttcg tgaaagccac cgggaagtgc gcggaatgcc
     2101 acgcgcacca gcaatactcc gtggtccacg aatatgaaat gagcaagcac gcggcgaaaa
     2161 gcgtgacttg tctggattgc catcagccag cacagggcca ggagaagaag gaacacaacg
     2221 gatttgtcat caccacgcac atgacggcgg gcaactgccg gccatgccat gagcaagttt
     2281 atcagcagtt cgtgcgcagc cggcacgccg ctccgtcgtg ggcggcggtc atgggagaga
     2341 aaggcttgac cgtggagcag gcggactttg cggagaaata caatcccgga tatgtaagaa
     2401 ggcctgcgaa tgcgctcgcc gtgctcgagg gcacctccgc catcagcggc ggctgcggcc
     2461 agtgccacgc gatcggcaag ccgaatgaag atggcacaat cggaaactgc accgcatgcc
     2521 atacacggca cactagctcc gtggccattg cccgccggcc gagcacttgc ggccagtgtc
     2581 acatgggacc cgaccattcg cagattgaga tctacgaaga gtcgcggcac ggcgtcctat
     2641 tcgccgcgca agagcgccag ctcaatctgg atgccgaccc gaagtcgttg accacccgcg
     2701 atatgttcgt tcctacttgt gcgacctgcc acatgtcggg gatcaacggg ctgaaagtga
     2761 cgcatgatcc gggtgagcgg ctctcctact atcttgcgga tgccatttcc aagcaacggc
     2821 caaattatct tctggcacaa gccaacatga agcagatctg caaccagtgc cactcgaaaa
     2881 cgctgatcga tcgcgtttac aatcaggccg aggacgttgt gaagtccaca aacgagaaag
     2941 tccaaagagc tcaggaactg gtcgctggtc tgcgaaagga aggaagcctt ggatcgcaac
     3001 ctttccagaa acccatcgat ttcctgctgt tcgatctttg gcactatgac ggcaggactt
     3061 cgaagcacgg cgcgttcatg ggtggagccg acttcgtgca gtggcacggc aactacgaat
     3121 tgctgaaaaa gcaggcgcag ctcgaagcca tggcggagga actccgtgcc aagcatggcc
     3181 acaagtgaag ttcccgtgaa tggtcagagc gaatgggtcg gggcggaaga tctgtggctc
     3241 gaagtgtttg tcatcttcaa ttttgtttgt cttacaggcg acatagctat cgcgcacgcc
     3301 gcaaatcatt tccggaacgc ggctgaatac attcccctgt ggttttcgtt gctggccgct
     3361 gccgtcctcg tcgtcggcat cgcgggccgt ttgcgcaaca accggcaatc cgtctggaga
     3421 gatttgggat atctcgttgg ttggatgtcc atcgccgtcg gggcggccgg agtcgtctac
     3481 catctgaata gcagcttctt ctacgaacgc acactgaaga gcctgaccta cgctgcacca
     3541 tttgcggcgc cagtcgccta catgggcctg ggatgtctgc tgctcatgaa ccgcatggtc
     3601 gcgcgtcaca ccagagagtg ggcgcagtgg gtgcttttct ttaccctcgg tggatttgtc
     3661 gggaattttg tcttgagcct gagcgatcac gccgtcaatg gcttttttcg ctggagcgaa
     3721 tggatccctg taatcagcag cggcttcgcg gtgggttttc ttctcgcact gctgctactg
     3781 gatgagcggc gtggatttct ctggcttact gcgggaattc tgtttgtgca gatactagtc
     3841 ggcggactcg gttttctcct gcacgcatgg gccgactggc acggacctgc ggggagtctc
     3901 tttcaaaata tcgtcagcgg tgctccccct tttgctccgt tgctccttcc caatctctcc
     3961 ctactgggat tcatcggaat ccttgcgatg gatcgcgcaa ttcctaaata agcagaagcc
     4021 ggcggattct ggcggtgaga tctactttgc tgcggttgtt tggcgcgagc ttgccgcagc
     4081 caattcaagg aagtggcgat ggatggtggt gtcatctgtc aactccggat ggaacgtcgc
     4141 cgccatgatg ttgtcacgct gcacaagagc gggcttccca gcgtattccg cgagaacttt
     4201 cacgcccggc ccggcccgct cgataatggg gccgcgaatg aaaaccattt ccagcggctc
     4261 gctcttcagt ttggtagggc cgctaaccac gtcgctggcg atctgccgcc cgtagccatt
     4321 tcgcagcacg gtcatatcca gcagtttcag cgaatcctgc ttcggattct ggacctccgt
     4381 ggcaagtagt atagctcccg cgcacgttcc aagaatcgct ccaccctgct ttgcgaattt
     4441 cgcaatcgcg tccgccagac cctcttcctg caggaactgc aactgtgtcg tgctctcgcc
     4501 gccgggcaaa atcaagccgt cgcagccctc gagttgggca ggagtgcgca cttccgccgt
     4561 ttccgcgccc agccgcgcca acattgccgc atgcgcttca aagtctccct gtaccgccag
     4621 aatcccaatc ttcacttcac gcactccaca ttttcgttgt aggggcgcag catgctgcgc
     4681 cccttacgtc caggttgaaa atacttgagg atcgttcacc atccgcgcgt cgaaagcatg
     4741 tccttctctt ccatctgccg gatatccaga cccggcatgg cttcgcccag ttcttcgtga
     4801 gcttcgagta cggccttcgg atcttcgaaa tgcgtcgccg ctttcacaat cgccttcgcg
     4861 cgagtctcgg gatcgctgga cttaaagatg cccgatccga cgaaaaccgc ttccgcaccc
     4921 aggcgccgta ccagcgccgc atccgccggt gtcgcaatcc cgcccgccga gaaattcggc
     4981 accggcagct tgccagtctt ggcaatctgg cgaatcagtt cgagaggcgc gcccaattct
     5041 ttcgcttcgc gcatcagctc gtcgtctctc atggtggtca gcttcttcat ctggctgacg
     5101 atcgcgcgca tgtgccgcac cgcctccacc acatttccgg agcccgcttc gcccttggtg
     5161 cgaatcatcg ccgcgccttc cgcgatgcgc cgcagcgctt cgccaagatt gcgcgcgccg
     5221 caaacgaacg gcacgcggta atcgtgcttc cagatgtggt tctcttcgtc ggccggcgtc
     5281 aacacctcgc tctcgtcaat gaagtccact tccagcgcct gcaacacatc cgcttccgcg
     5341 aagtgtccga tgcgcacctt ggccatcacc ggaatcgtca ccgtggccat gatttcgcgg
     5401 atcactttga tcgacgccat ccgcgccact ccgccctctt tgcgaatatc agaaggcacg
     5461 cgctccagcg ccatcaccgc gcatgcgccg gccttttcgg cgattttcgc ctgctccgca
     5521 ttcgtgacgt ccatgatcac gccgcccttg agcatctccg ccaggccgac tttcacgcgc
     5581 cactcattcg atcccttgct tccgttgccg ctgccgttga ccttcttcgc atccattttg
     5641 cttctcctct cgctgctcga acttcccaac gatgtttctc cctcaaatct ttttcacttc
     5701 tgcccgatca ctacgaatct cgctctgccc ctcgccagca ctatcccatc cgtatcgcga
     5761 atctcaccgg tcataaaaat gtttcttccc ttttgctccg tctcgcgccc ctccacctca
     5821 atttctttca tcacgtcgac cggcttcagg tactcgaccg tcatttccgc cgtcaccgcg
     5881 cgaacctcgc gaaaccggca gaccttcccc atcgcttcat ccaacagcgt cgcgataatt
     5941 cctccatgcg caaatcctcc gccgccctga tatctctcgc ccaaaacaaa tcgccccaca
     6001 atccgccgat tcacgttgtc ctgctcgaac gtcagcttca tcccgccagc attgtctccg
     6061 ccgcagccaa agcacttgtt cgtcggattc ggcgccaacg taattgtttc gctcaaatgt
     6121 cgccctcgct ggcctttcct tatttcgtca cttccttgct tctttccttc attgcttcgt
     6181 tctctcagac cagtgcgact cggctccgct ccgcccgtcg aaccccgcgc tgccgcttgc
     6241 gcatttcaat cttcagcaat tccgccagtg tcgccacgcc tcgcgcgatc tgcttttcat
     6301 ccaggctggc aaagcccagc cgcagcgtgt tcggctgcgg cgtttgcacg tagaagtacc
     6361 gtcccggcgc aaacagcacg ccacgttctt tcacgtgaat cagcaattcg ctggcatcga
     6421 accccagtgg cagctccagc catagacaca tgccgccctc cggcttggtc cagcgtgtgc
     6481 cctcgggcat gtgtttccgc agtgcttcat ccaatgccgc cagccgcgag ccgtaaacct
     6541 tgcgcatctt ggcgagatgc ttgctgaaaa gtccgcgccg cagaaattcc gcgagcgtag
     6601 cttgtgccag ttgatccgta tgcaggtcgg tcgtctgctt caccacgcgc aaccgttcaa
     6661 tcgccgtagc cggagcgacg atccacccaa cccgcaaacc gggaaatgcc actttcgcga
     6721 agctgtcgat atgaatgacc aggttcgagc ggtcgagctg cttcagcgat gggatgcgtt
     6781 cctccctcgc atgcagccgc gcgtagatgt gatcttccac taccgggacc tgatggcgcc
     6841 cggccagctc cagcaccttg cgccgcgatg ccagcggcat cgatgttccg gtcggatttt
     6901 ggaagtccgg ggtaagaaca atgagcttga cgcgattggc cgccagggtt gcctccagcg
     6961 cctctaggtc cagacccagc ggcgtccccg gttccgcatg agtgttcatg ggaacgctca
     7021 ggcaccgcgc gcgtgcgcca tggaagatcg ccaccgctcc cggatacgtc gggttttcca
     7081 taatcacgga atcgcccggg cgcacaaacg ctttgctgat cagatcgaag gactgctggc
     7141 atccatcggt gatgagcagg ttttcatctt tggccgcgat gccgtcgtga tggagcatct
     7201 cgagcagcgc cgctttcaag ggcggatagc catccgacgg accgagattc aacacttccg
     7261 cggcttctcg ccgaagcacc gcgttcacgc aagtctgcag ttcttcgatg gggaaaaatt
     7321 cctgcgccgg ccgcgccatc acgaaggaaa gcgcatcttc gggcacattg gcagtgagcc
     7381 ggctaaggac ctcttccccg cgttcatcag caaagagcag ttcccaccga attcccccgt
     7441 tgccattcag gaccggaggc gcgggcggcg tgatcttcag tccgttgccg tttgccctga
     7501 tgaacgtgcc ccgtcccacg tggccctgaa tcagcccttc cgattccagc tcggcgtaag
     7561 catttgccac cgtcgtgcgg tgcacgccga gcatggtcgc gagttcacgg ctggccggaa
     7621 tgcgatcgcc cgggcgcaag tcgcccgcgt gaacgagggc gcgcagttga tcgcgaagtt
     7681 ggatgtacag cggaacgtgg ctctgtggct ggagatggag tggaaacatt gtctctcacc
     7741 ttcggccaat tctaacagat attggcctgg tacaagagcc aatttgtctc cacccagcga
     7801 tccttatgtc cgccccagaa ccagccgg
//
LOCUS       DASYVO010000232         9858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_90856, whole genome shotgun sequence.
ACCESSION   DASYVO010000232 DASYVO010000000
VERSION     DASYVO010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9858
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90856"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VGR03_07700"
     CDS             <1..523
                     /locus_tag="VGR03_07700"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEV1994198.1"
                     /translation="LSWERLVHFLESTSIVSVERGAQLLSLPQSDPQAAEALLLKARR
                     LGSALRKVFAAMLRKQRIAGDWIEPVNEVLRITEGHDELVAQGEVWKIEFVAREGGLN
                     WLLAAVARSGAEIIAEGARARLRVCANPHCGLFFYDNSRTRRRRWCSMAVCGNRSKVA
                     AFARKHTSARNTP"
     gene            complement(566..2059)
                     /locus_tag="VGR03_07705"
     CDS             complement(566..2059)
                     /locus_tag="VGR03_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEV1994199.1"
                     /translation="MKSCGSNSGAAYSRPVRALVGTARKVASGNRRGGLMGRWVSLHR
                     FALILCASLCFAITSRGSAGSAPSAPPETPAAQASPQQAGSSQSSSSESKTPEQTRTE
                     QYTLSHERQAKAVAYSQVGYTLYFVSYFLGGLFLFLILRLGWAAKFRDIAENASDKKW
                     IQGLVFVPLLFLTIDVLDLPLSVYWHSLSLHYEQSVQGWGSWFWDWTKGEFLGTAFGI
                     VLVLILFAVMRRSPRRWWFYFWFPAVLILLGLIVITPLVIDPLFNKFEPLSKEHPDLV
                     ASIEKLTKHAGVPIPPHRMFLMAASQKTNAINAYVTGLGASKRVVIWDTTIQKTSNDE
                     SLFIVGHELGHYVLGHVRQGFLLGAAGLLLALYFLFQGLHWALDRWARDWKLYGQEDW
                     ASLAVLLLLLQVLLFVSSPVISGYSRMQEHAADVYGLEVIHGLVPNSEEVAAHAFQVL
                     GELDLSDPNPPPFITFWLYSHPPLADRLVFAHSYDPWSKGESPKYVK"
     gene            complement(2120..2599)
                     /locus_tag="VGR03_07710"
     CDS             complement(2120..2599)
                     /locus_tag="VGR03_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994200.1"
                     /translation="MLKLRMILLLALAAFMWTNKVCAQAPATEAQTPSPVVVPTAGKN
                     QTRARMQEHRFWDKKNDWLFAGVGTARTFDYFSTLNLRRRGDQEILVTNNLVDNHAAF
                     AMVEAAGTGVSLGVSYLFHHYGHHKLERWTSIAHFGLATTGAVRNYCLRTAHPKTTP"
     gene            2700..3743
                     /locus_tag="VGR03_07715"
     CDS             2700..3743
                     /locus_tag="VGR03_07715"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEV1994201.1"
                     /translation="MTRFRLVLSSILVFALVAVVAAHAVRGTAAETSGAVPARSFEFT
                     YQVHVPANADSAGPTHLWIPLPQADGYQDIRGLYIDSFVSYVQGRDLEYGNGFAMFTP
                     SPPQSASGFDVTVRFTATRRKHLVPLTLGVYPAAKPDPMLQRYLQPDKLVPLNSTIAE
                     LAKEQTTGASTPLEKARYIYDYVVATMRYDKTGEGWGRGDALWACTSKRGNCTDFHSL
                     FIGMMRSSGIPARFEIGFPLPENKSEGDIPGYHCWAEFYVGGVGWVPVDASEAWKNPA
                     KRDYFFGAHDTNRIFFTYGRDIRLSPDQKGDPLNYFIYPYAETNGQPVKNLQTHFSFR
                     DVSAAQLAAAGAN"
     gene            3913..4500
                     /locus_tag="VGR03_07720"
     CDS             3913..4500
                     /locus_tag="VGR03_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994202.1"
                     /translation="MFRCIPLRLALLLVLATALLPAAAFAQSQDTQSVAEAARRARAQ
                     KKAQTNSAKVITNDDVKPAAQSGPATPGSPDATAASGTPALSTANAGSAPAAGSAPAP
                     DGKDQKDSKELTELKAQIKQVQGDLDLLQREQSLENDRYYSNIDYAHDTAGKAILDEL
                     KHLVSDKQQELDRLKARLAELQPSQSGTPSTPPKS"
     gene            4643..5539
                     /locus_tag="VGR03_07725"
     CDS             4643..5539
                     /locus_tag="VGR03_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1994203.1"
                     /translation="MQLQVENLSKNYRGGVQALRNVQISLRPGILGLLGPNGAGKSTL
                     MRILATITQPSSGRVLWNDADIARDPDALRNVLGYLPQDFGVYPNLNAIEFLEYLAAV
                     KGISADAAKKRIGELLELVNLGDAAKRPLGGYSGGMRQRAGIAQALLNDPQLLIVDEP
                     TAGLDPEERVRFRNLLSELSGERIVILSTHIVSDVEAVATSIAILAHGQLLAQDSPEG
                     LLASVSGKVWEVVVSSAELPALRQKHLISNTAHRSDGVHARVVANVVPGAGARPLEPS
                     LEDAYLAALAAQRMNHQAGAAA"
     gene            5536..7122
                     /locus_tag="VGR03_07730"
     CDS             5536..7122
                     /locus_tag="VGR03_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994204.1"
                     /translation="MTALRSIYAVAHADFLERVRRYSFLVTLLFAVCLGYATAKGKIS
                     LQLDEYRGVYTSAWIGTLVALVTACFVSLVGFYIVKNSVERDRSTGVGQILAAMPLSK
                     LSYAFGKFLSNFAVLTAMLAVLALSALAMQFLFAEDPRVDLWALLAPFLLMALPVMAL
                     TAAIALFFEMVPVLSGGVGNVVWFFVWIFGTALPLISGLRWLDPVGIFTVMRSLSAEA
                     RRYVPGYHGGMAFQIDFGQIRVAQDLRWPGIPWTWDAILLRLMWFGVAAILALFAALV
                     FDRFDSARAGASSIRKHKGELSTAPAAGAASVAPPVTAAIHLTPLATGAHTNAFGRLF
                     IAELRLALKGSRWWWYAIAAGLLVAQIFSPLDVARGPLLATAWFWPVLIWSAMGTRES
                     RFGTRGLLFSSPRILLRQLPACFLAGFAVAALTGSVVAVRLLIAGQQVGLIAWLAGAL
                     FLPSLALASGIVSGTGKVFEALLTVLWYIGPMNHTPGFDFTGAANGPLMTHYACVYTA
                     MSAALLFFALFTRARQLRSN"
     gene            complement(7188..8219)
                     /locus_tag="VGR03_07735"
     CDS             complement(7188..8219)
                     /locus_tag="VGR03_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994205.1"
                     /translation="MNLKRTIGLILVIAGVILGVAKALGGETQSGDWTIRRSDAPGKV
                     EFSLMDSRAGHHFHTSSDWPASDFSGLDFSKAGRQEVHFTIARDAGKFECEGFLQDGE
                     GAGLFHFVADPKYPQEMKSLGLGGIDGDKQWAMAIHDVSLKFAKDIKAENLEGLDTDK
                     LIAFKIHGVSPEFIAGIRAAGVNESDSDKLIAFRIHGVTPEFVSGIRSAGLNVLDSDK
                     LIAFRIHGVTPEMVRSLKQAGYDPDADKLIAMRIHGATPEWVAELKKQGYEHLDLDKL
                     IAFRIHGVSPDFIGQLQKLGYSHPEPDQLIAMRIHGVTPDFITGLQSRGMKNLSVEQL
                     VSLRIHGID"
     gene            complement(8254..>9858)
                     /locus_tag="VGR03_07740"
     CDS             complement(8254..>9858)
                     /locus_tag="VGR03_07740"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEV1994206.1"
                     /translation="QTNPAVQTGAEGASTWAGTSTQNATALSGSRAPVAESRSEQSIA
                     MLWMVEAWFLGVLLLSLRTAGGLFLIERMRRNEIKPVGAELYARCLALQKRMGLDRVI
                     QYCECHRLDAPAVLGWFRPVVLLPVRALTGLTEEQIEAVISHELAHIRRFDCFVNLFQ
                     IATETLLFYHPAVWWVSQRIRAERENCCDDEAIAVCGDAVNYARALTLMEEWRTAPEL
                     MMAANRSPLAERVVRLLGWDGAAGRIRVAGLAGSFVCLVGALLAGNAFLGVAHAALGG
                     AASPKQEQGSSSVIVVRPEPASAKDRTAQAAKPSGSGEAKAQTNKSKDQQAASEEQNK
                     KESYLDAMEAAGFKNLSVDELISMKIQGVTPGYVKEMRELGLQPTPDELVGMRVQGIT
                     PEYIRGMRTFYSNLNIDELIGMKVQGVTPEYIREFHELGLQPSADDLIGMKVQGVTPS
                     YVREMRATGLKPNTDELIGMKVQGISPEYVKSMQAAGFKDLDCDELIGAKVQGITPEF
                     IDKARKHGFQSLTLDKLMALKHADIL"
BASE COUNT         1939 a         3137 c         2628 g         2154 t
ORIGIN      
        1 actgtcctgg gaacggcttg ttcactttct ggagtccacc agcattgtct ccgttgagcg
       61 cggtgcgcag cttctgagcc tgccccaatc cgacccgcaa gctgcggaag cactgctctt
      121 gaaggcccgg cggctcggct ccgccttgcg caaggtgttc gcggccatgc tccgaaagca
      181 aagaattgcc ggggattgga tcgagccggt aaacgaagta ctgcgtatca ccgaagggca
      241 cgatgaactt gtcgctcagg gcgaggtttg gaagatcgaa ttcgtcgcac gtgagggagg
      301 cctgaactgg ttgctggccg cggtagcccg atcaggagcg gaaattatcg ccgaaggagc
      361 ccgcgcgcgc cttcgggttt gcgccaatcc gcattgtggc ctcttcttct acgacaattc
      421 ccgcacgcgc cgccgccgct ggtgttcgat ggccgtttgc ggcaatcgga gcaaagtagc
      481 tgcttttgcc cgcaagcaca catcggcgcg caacacgcca tagagagcag ccctggttca
      541 ccaagggctt cgtacacgga cacggctact tcacgtactt cggcgattcc ccttttgacc
      601 atggatcgta gctatgcgcg aacaccaagc gatccgccag cggcggatgc gagtagagcc
      661 agaaggtgat aaacggcggc ggattggggt ccgatagatc cagctcaccg agaacctgga
      721 acgcgtgcgc ggcgacctcc tccgaattcg ggaccaaccc atggattact tccaatccgt
      781 aaacgtcagc cgcatgttcc tgcatccggc tatagccgct gatgaccggc gaagatacga
      841 acaacagcac ttgcagaagc aagagcaaca cagcgagcga cgcccaatct tcctgcccgt
      901 atagcttcca gtccctggcc cagcgatcca gcgcccagtg caagccctga aagaggaaat
      961 aaagcgccag caatagcccc gccgctccca gtaaaaaacc ctgacggacg tgtccgagga
     1021 catagtgtcc gagctcatgt ccaacgatga agagggattc atcgttggaa gtcttttgaa
     1081 tcgtggtatc ccagatcacc acgcgcttcg acgctcccag gcccgtcacg tacgcgttga
     1141 tggcgttcgt tttctggctc gctgccatca ggaacatgcg gtgcggcgga atgggaacac
     1201 ccgcgtgttt cgtgagtttt tcaatggaag caaccagatc gggatgctcc ttgctcaatg
     1261 gctcgaactt gttgaacagc ggatcgatca ccagcggggt gatcacgatc agtccaagga
     1321 gaatcaagac cgcaggaaac cagaaataga accaccagcg ccgcgggctg cgccgcatca
     1381 cggcgaacaa gatcaggacc agcacgattc caaacgccgt gccgagaaac tctcccttgg
     1441 tccaatccca gaaccacgag ccccaacctt gaacggattg ttcgtagtgc aaggaaagcg
     1501 aatgccaata cacgctcagg ggcaggtcca gaacgtcgat ggtcaggaaa agcagcggga
     1561 cgaaaaccaa tccttgaatc catttcttat cgctggcgtt ttcggcgatg tcgcgaaact
     1621 ttgccgccca acccagccgc aggatcagaa aaagaaaaag tccgccgaga aaataagaga
     1681 cgaagtaaag cgtgtagccc acctgcgagt acgccaccgc cttcgcctga cgctcatgcg
     1741 agagcgtgta ttgttcagtc cttgtctgtt ctggagtctt cgattccgaa gacgaactct
     1801 gcgaggaacc ggcttgctgc ggcgaggctt gcgcagccgg cgtttctgga ggcgcggatg
     1861 gcgcggagcc tgcagaaccg cgcgatgtta ttgcgaaaca tagactcgca caaagaatca
     1921 gcgcgaaacg gtgaagggag acccaccgtc ccataagacc acctcgccta tttcctgacg
     1981 caactttccg tgcggttccg accagggccc gcacgggccg cgagtatgcc gcaccgctat
     2041 tcgaaccgca agatttcatc tcactgattt agacggggag gactagagag cgtttccgcc
     2101 tgatgggcgg cgcgcccggc tacggagtgg tctttggatg tgccgtcctc agacagtagt
     2161 tgcgcacggc gcccgtcgtc gccaatccga aatgcgcgat cgaagtccac cgttccagtt
     2221 tgtgatggcc atagtgatgg aacaagtacg acaccccaag cgaaacacca gttcccgccg
     2281 cctcaaccat tgcaaaggcc gcatgattgt ccaccagatt gttggtaacc aggatttcct
     2341 ggtctccgcg ccgcctcaag ttcagcgtcg aaaaataatc gaacgtccgc gccgttccga
     2401 cacccgcaaa caaccagtcg ttcttcttgt cccagaagcg gtgctcctgc attcgtgccc
     2461 tggtctgatt cttgccagca gtcggcacaa cgactggcga tggtgtctgt gcttcggtcg
     2521 ccggcgcctg ggcgcaaact ttgttcgtcc acatgaaagc tgccagagct agcagcagga
     2581 tcatcctaag ttttagcaat tcttccgctc ccgggggtat tgcgtttgtc ttatttctca
     2641 attcagtata atcgcacggg cgctattctg ggccacactc tccacgcagg agtccttcca
     2701 tgacccgctt ccgtctcgtg ctctcgtcga ttctcgtttt cgcgcttgtt gccgtggttg
     2761 ccgcccatgc ggttcgcggg accgccgcgg agacgagtgg agcggtgcca gcgcgctcgt
     2821 tcgaattcac atatcaggtg catgttcccg ctaacgcgga ttccgcgggc cccacccatc
     2881 tctggattcc cttgccacag gccgatggct accaggacat tcgtggcttg tacattgaca
     2941 gcttcgtgag ttacgtccag ggccgggatc tagaatacgg taatgggttt gcgatgttta
     3001 cgccttcgcc gccgcagtcc gcttcgggct tcgacgtgac cgttcgcttc accgccactc
     3061 gccgtaagca ccttgtgccg ctcaccctgg gcgtctaccc cgcagcgaag cccgatccaa
     3121 tgcttcagcg ctacctacag cccgacaaac tcgtcccgct caacagcacc atcgccgaat
     3181 tagccaaaga acaaaccact ggggcctcga cgcctctcga aaaagcccgc tacatctatg
     3241 actacgtcgt ggctactatg cgttacgaca agaccggtga aggctggggc cgcggagatg
     3301 ccctctgggc ctgcacctca aagcgtggca actgcaccga cttccattcc ctcttcattg
     3361 gcatgatgcg ctcgtccggg attccagcgc gcttcgaaat cggcttcccc ctgcctgaaa
     3421 acaagagcga aggcgacatc ccgggatatc actgttgggc tgagttttat gtaggcggag
     3481 tcggttgggt tcccgtggat gcttccgagg cctggaagaa tcctgccaag cgcgattatt
     3541 ttttcggcgc gcatgatacg aaccgtatct ttttcactta tggccgcgat atccgtctct
     3601 cgcctgacca gaagggcgat cccctgaatt attttatcta tccctacgcg gaaacgaatg
     3661 gccagccagt gaaaaacctg cagacacact tttcgttccg cgacgtttcg gcagcgcagc
     3721 tcgccgcggc aggcgccaac tgattcctgg tctcatcaag gctcgtatcg agttttcgca
     3781 cataaagacg catgcgttct gcttcactcc gttggctcat tcgcactcgt tcgctacttg
     3841 ggattgtcat tccactcctg ccggcgtatc ttgagaattg acccagctca ggttttcatg
     3901 aggagtagca ctatgtttcg ctgcatccca ttgcgccttg ccttgctgct cgttctggcg
     3961 acagcgctgc ttcccgcggc tgcctttgcc caatcgcagg acactcagtc cgtggccgaa
     4021 gccgcccgcc gcgcccgcgc gcagaagaaa gcgcagacga actccgccaa agtcattacg
     4081 aatgacgatg tgaagcccgc ggcgcagtcg ggtcccgcaa cgccgggttc gcccgacgcc
     4141 acagcggcct ccggcacgcc tgccctatca accgcaaatg caggtagcgc gcccgccgcc
     4201 ggctctgctc cggcgccaga cggcaaggat caaaaggatt cgaaggaatt gaccgagttg
     4261 aaagcccaga tcaaacaagt ccagggcgat ctcgatctgc ttcagcgcga acaatcgctg
     4321 gaaaatgaca ggtattattc gaacatcgac tacgcccacg atacggctgg aaaagcgata
     4381 ctcgacgaac tgaagcacct ggtcagcgac aaacaacagg aactcgatcg cttgaaggcc
     4441 cgcctggcag aattgcagcc gtcgcagagc ggcaccccaa gcaccccgcc gaaatcgtag
     4501 ttttcatcca ttccattcct cagttacaac ttctccatct tcccgcaccg ggataattcc
     4561 gtgtttccag caaacttttt gctactctcc tgcgtattct ctctagcaag aacgagcggt
     4621 cgatcccgcg gaatggattc tcatgcagct ccaggtcgaa aatctcagca agaattatcg
     4681 cggcggcgtc caggcgctcc gcaacgttca gatttctctg cgccccggca ttcttggact
     4741 tcttggcccc aacggcgcgg ggaaatccac tctgatgcgc attctggcaa cgatcaccca
     4801 gccgtcctcc gggcgagttc tctggaatga tgcggacatc gcgcgcgatc ccgatgctct
     4861 gcgcaacgtt ctcggctatc taccgcaaga ttttggtgtg tatcccaatc tcaacgcgat
     4921 tgaatttctg gagtatcttg ccgccgtaaa aggcatctcc gcggacgcgg caaaaaagcg
     4981 catcggcgaa cttctggagc tcgtaaatct cggagatgcc gcaaagcgcc cgctgggagg
     5041 ctactccggc ggcatgcgcc agcgcgccgg catcgctcag gcgctgctca acgacccgca
     5101 actcctcatc gtcgatgaac ccaccgccgg ccttgatccg gaggaacgcg tccgcttccg
     5161 caatctcttg tccgagcttt cgggcgagcg catcgtgatt ctctccacgc acatcgtctc
     5221 cgatgtcgaa gccgtcgcca cgagcatcgc catcctcgcg catggccagc tcctcgcgca
     5281 ggactcgccg gaaggactcc tcgcgagtgt ctccggaaaa gtttgggaag ttgtcgtctc
     5341 cagcgccgag cttcccgctc tgcgtcagaa acatttgatc agcaacacgg cgcaccgcag
     5401 cgacggcgtt cacgcgcggg tagtggcgaa cgtcgtgccg ggcgcgggag cgcgcccgct
     5461 tgaaccgtct ctcgaagacg cctacctagc cgcgctcgct gcacagcgca tgaaccatca
     5521 ggcgggagca gcagcgtgac ggctcttcgt tcgatttacg ccgtcgctca cgccgacttt
     5581 ctcgaacgcg tccgtcgcta cagcttcctc gtaacactcc tctttgcagt gtgtctgggc
     5641 tacgcgacgg caaaagggaa gatctcattg cagctcgacg agtatcgagg agtgtatacg
     5701 tcagcttgga ttggaacact ggttgccctt gtaacagcct gctttgtttc cctcgtcggc
     5761 ttttatatcg tcaagaactc cgtcgaacgc gaccgttcca caggggtggg ccaaatcctg
     5821 gcggccatgc cgctttccaa gctctcctac gctttcggga agttccttag caattttgcc
     5881 gtcctcacag cgatgctcgc ggtgcttgcc ctctcggcat tggccatgca gtttctgttt
     5941 gcggaagacc cgcgagtcga tctttgggca ctgttggctc cctttcttct gatggctctt
     6001 ccagtcatgg cgcttactgc agcgatcgcc ctctttttcg agatggtgcc agtgctgagt
     6061 ggtggcgtgg ggaatgtcgt ttggtttttc gtatggattt tcgggacggc gcttcccttg
     6121 atatccgggc ttcgctggtt agatccggtc ggaatcttca cggtgatgcg gtcgctcagc
     6181 gcggaggccc ggcgatatgt gcccggctac catggcggta tggccttcca aattgacttt
     6241 ggacagatcc gcgtcgccca ggatttgcgc tggccaggca tcccgtggac ttgggacgcg
     6301 attctgctcc ggttaatgtg gttcggggtt gctgcaattc tagcgctttt cgctgcgctt
     6361 gtgttcgacc gcttcgactc cgcccgcgct ggcgcttcct ccatccgcaa gcacaaggga
     6421 gaactatcca ctgcgcctgc cgcaggcgca gcgtctgtcg ctccgccggt caccgcggcc
     6481 atccatctca cgccgctcgc taccggcgca cacacgaacg ccttcggccg cctcttcatc
     6541 gctgaattgc gtctcgccct gaaaggatct cgctggtggt ggtatgcgat cgctgccggg
     6601 cttcttgtcg ctcagatctt ctcgccgctg gacgtggcgc gaggacccct gctcgccacc
     6661 gcctggtttt ggcccgttct catctggtcc gccatgggca cgcgcgaatc gcgcttcggc
     6721 acgcgggggc tgctgttttc ttctccgcgg attctgctgc gacagcttcc cgcctgcttc
     6781 ctggctggct ttgcggtagc ggcgctcacc ggctcagtcg ttgcggtgcg tttgctgatc
     6841 gccggtcaac aagtcggcct gatcgcatgg ctcgcgggag cccttttcct tccgtcattg
     6901 gcgctggcct ccggcatcgt ctccggaacc ggcaaagtct ttgaagccct gctcaccgtt
     6961 ctttggtaca tcggcccgat gaatcacaca ccaggcttcg atttcaccgg tgccgccaac
     7021 ggtcctctca tgactcacta tgcttgcgtt tacaccgcga tgagcgccgc gcttcttttc
     7081 ttcgctctct tcacacgcgc gcgccagctc cgctcgaact agcgagtaga gattctcctc
     7141 ggttgccggc tttctggttt ttctattgat gcaaacggcc ggaaaaatca atcaatccca
     7201 tgaatcctca ggctgaccag ctgttcgacg ctcaggttct tcattccacg cgattgcagg
     7261 ccggtgatga agtcgggcgt cactccgtgt atgcgcatgg caatcagttg atccggttca
     7321 ggatgagagt atcccagctt ctgaagctgg ccgataaaat ccggcgacac tccatgaatc
     7381 cggaaggcga tcagcttgtc gagatcgaga tgctcgtaac cctgcttttt caactccgct
     7441 acccattccg gcgtcgcccc atgaattctc atggcaatga gcttgtccgc gtcaggatcg
     7501 tagccggcct gtttgaggct gcgaaccatc tccggtgtca ctccgtgaat ccggaaagcg
     7561 ataagtttgt cactgtccag gacattcagc cccgccgagc gaattcccga aacgaattcc
     7621 ggagtcacgc catgaatgcg gaaagcaatg agtttgtcgc tgtctgactc gttcacgccc
     7681 gctgcccgaa tgcccgcgat gaactcggga gatacaccat gaatcttgaa agcaatcagc
     7741 ttgtccgtgt ccaacccctc caggttttcc gctttgatgt ccttggcaaa cttcaagctg
     7801 acatcgtgga tggccatcgc ccattgtttg tcgccatcga tcccacctaa gccgagcgat
     7861 ttcatctctt gcggatactt cggatcagct acgaaatgaa acagcccggc gccctcgccg
     7921 tcctgtagaa acccttcgca ttcgaatttt cccgcgtccc gcgcgatcgt gaaatgaact
     7981 tcttgccgcc cggccttgga aaaatccagc cccgaaaaat cgctcgccgg ccagtctgaa
     8041 ctggtgtgaa agtgatgccc ggcacgcgaa tccatcaaag aaaactccac cttccccgga
     8101 gcatcggagc gccggatggt ccagtcgccg ctctgcgtct cgccgccgag agctttcgcg
     8161 acacccagga tgacgccggc gatgacaagg ataagaccga tggttcgttt tagattcatg
     8221 ggatctcact cctctccgta tttcctgtga aactcacaaa atgtctgcgt gcttcagcgc
     8281 catcagcttg tctagagtca gactctgaaa cccatgcttc cgcgccttgt cgatgaactc
     8341 cggcgtaatc ccctgcactt tcgcgccgat cagctcgtcg caatccaggt ccttgaagcc
     8401 tgccgcttgc atcgatttca cgtactccgg cgagattccc tgcaccttca tcccaatcaa
     8461 ctcatcggta tttggtttaa gtcccgtcgc gcgcatctcc cgtacgtagc ttggcgtaac
     8521 tccctgcacc ttcatcccga tgaggtcatc ggcgcttggt tgcagcccta actcgtggaa
     8581 ttcgcggata tattccggcg taactccctg cactttcatg ccaatcagtt catcgatgtt
     8641 gaggttggaa tagaaagtcc gcatcccacg aatatattcc ggcgtgatgc cctgcacgcg
     8701 catgccgacg agttcgtccg gcgtgggttg caaccccagt tcgcgcatct ctttcacata
     8761 tcccggcgtg acaccctgaa tcttcatcga gatcagttcg tccaccgaca ggttcttgaa
     8821 gcccgccgcc tccatcgcgt ccagatagga ttccttcttg ttttgctcct cgctggccgc
     8881 ctgctgatcc ttgcttttgt tcgtctgcgc cttcgcctcg ccagatcccg agggttttgc
     8941 ggcttgcgcg gtacgatcct ttgccgaagc aggctccggc ctgacaacaa tcacgctgct
     9001 gcttccttgc tcctgtttcg gagaagcggc acctccaagc gcggcatgcg ccacccccag
     9061 gaaggcgtta cccgccagca gcgctcccac cagacaaacg aagctgcccg ccagtcctgc
     9121 cacgcgaatt ctgcctgcag ctccatccca tccgagcagc cgcaccaccc gctccgcaag
     9181 cgggctgcga ttggccgcca tcatcaattc cggagccgtc cgccattctt ccatcagcgt
     9241 gagcgcccgc gcgtaattca cggcgtctcc gcaaaccgca atcgcttcgt cgtcgcagca
     9301 attctcgcgt tcggcgcgaa tccgctggct cacccaccag accgccgggt ggtagaacag
     9361 cagcgtttcc gtggcgatct ggaaaagatt cacgaagcaa tcgaaccggc ggatatgcgc
     9421 cagctcgtgc gagataaccg cttcgatttg ctcttcggtc aagccagtca gcgcccgcac
     9481 cggcagcagc acgacaggac ggaaccatcc cagtaccgcg ggagcgtcca gccgatggca
     9541 ctcgcaatat tggatgacgc ggtcgagacc cattctcttt tgcaacgcca ggcatcttgc
     9601 gtacagctcc gcacccaccg gcttaatctc gttgcgccgc attctttcga tcaaaaacaa
     9661 tccccccgct gttctcaagc tcagcaaaag gacgcccagg aaccaggctt ccaccatcca
     9721 caacatcgca atcgattgtt cgcttcgaga ctctgcgaca ggggcgcggg agcctgacag
     9781 cgcggtcgcg ttttgggtgg acgttcccgc ccacgtcgaa gctccctccg ccccagtctg
     9841 cacggccgga ttcgtttg
//
LOCUS       DASYVO010000233        20900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_4073, whole genome shotgun sequence.
ACCESSION   DASYVO010000233 DASYVO010000000
VERSION     DASYVO010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 20900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20900
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4073"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..835
                     /locus_tag="VGR03_07745"
     CDS             251..835
                     /locus_tag="VGR03_07745"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEV1994207.1"
                     /translation="MNPIETLGLALGAGFSSGLNFYATVATLGFLHRFGVLSLPPGLQ
                     VLSHPWVLGIAIALYLLEFLADKIPYFDTIWDVIHTFIRPPAAALLAFAAAGGATPEW
                     RWGAALIAGGVALTAHGTKASARAAVNASPEPFSNWALSIGEDVLAVWLAWMATAHPL
                     ATTIVVAALVALSAFLLYHLFRFARRALQRLLAS"
     gene            complement(966..3023)
                     /locus_tag="VGR03_07750"
     CDS             complement(966..3023)
                     /locus_tag="VGR03_07750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV1994208.1"
                     /translation="MKGVILRCGLLGALLLGFGFAITAQESAKHPITFDDLIKLHRIA
                     EPQVSPDGKWVAYTVATPDMDANRNASNIWMVPTTGGSVTQLTQSGHDSSPVWSPDGK
                     TLAFLSSRSGDSQVYLLSMDGGEAHPLTKLSTGADMVKWSPDGKTIAFTSSVYPDCKD
                     DDCNSKRDAEKEKSKVKAHVAEQLLYRHWTHWNDGKRGHLFVVPADANAAPRDLTPGA
                     NYDVPPDERSGPGDISFSPDGKELCFTAVTDKMEAISTNGDLFIVPVAGGEAKRITTQ
                     PGFDGNPVYSPDGKFIAYHAQLTAEYEADRWRVLLYDRAAGRIENLTETFDCSAEELA
                     WSPDSKTIYFTAENETQKPVYAMAARFGAEPKKIIADTYNTAISFSADGKTLVFERTS
                     LTLPAELFAASSDGSKVRQLTHQNDSILSSVEMNAPETFWFDGAEGTKVQAMLIRPPR
                     FDATKKYPLLVLLHGGPQTMWSNAWGYRWNAQVFSAAGYVTLMINRRGSTGYGQKFTD
                     EITNDWGGRAYVDVMNGVDYTLKKYAFIDGTRMAAAGGSYGGYMADWIATHTGRFKAI
                     VSHASVYDKVSMFATEELWFEEHDMQGAPWTSPENYKKWSPSTYAGELGKFKTPTLVI
                     AGERDYRVPYTQSLELFSALQRQGVPSKLVVFPDEGHWVLKPQNSQFWYKTFLDWLST
                     YVK"
     gene            3376..4713
                     /locus_tag="VGR03_07755"
     CDS             3376..4713
                     /locus_tag="VGR03_07755"
                     /inference="COORDINATES: protein motif:HMM:NF005685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV1994209.1"
                     /translation="MPTSSGLFPRNFKKTFPIAIRGEGCWIVADDGRRFLDASGQAAV
                     VSIGHGVAEIGRAMAEQSSQIAFAHTSQFHTAPAEQLAARLLAMAPPNFRNGGRVYFT
                     SGGSEATETAIKLARQFHLETGQNDRYRVLSRRQSYHGSTLGAMSVSGNVARRASYEP
                     LLAEWGHIAPCFCYHCPYEKAFPQCELACADDLEVHLLEHEAGQAAAFIFEPVVGATL
                     GAAVPPEGYAARIAEICRKNGILLIADEVMSGMGRTRKPFAVQHWGIEPDMILVGKGI
                     ASGYAPLGAVLVSARVVEAFERGSGAFMHGFTYQAHPVCTAAGNAVLDYLESHKLFDR
                     VYTAGQSLLGALSPLQSHPNVGDVRGLGLLLSVEFVKDKATREPFPKEQNIAERIRHA
                     CLEESVLTYPTQGCVDGLRGDHILLAPPFTISREECALIARALQSAVTKVFSV"
     gene            4779..6974
                     /locus_tag="VGR03_07760"
     CDS             4779..6974
                     /locus_tag="VGR03_07760"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEV1994210.1"
                     /translation="MRLPLRTLAVSFISLLILTFALSAQLKQDAPPSSPILGFSPPHA
                     AAEHEVESTFQSIPSPEKAREWHRTFTAEPHPAASDRNNQLADFVAEEWRKQGWEDVT
                     LRRYDVLHSRPRSVSLEMIEPVHFTASLREDAYDVDPDTKNPAVSGSYFGYSASGDVT
                     AEVVYAHSGNPEDYDLLRKNGISVKGKIVLVRYSNPYSYRGFKALTAEREGAAALLIY
                     SDPAEDGSTRGKVFPDGPWGPESHIQRGAITYDYIVPGDPTTPGWASVAGAKHIAPEA
                     ALSLPKIPALPLSWRDAKPLLENMNGQEAPRDWQGALPITYRFSGAVKVHVKVDMDTS
                     MQPYTVVEARIRGSELPDEWVLLGNHRDAWAFGGVDPSSGTASMMELTRSLGELKQRG
                     IRPRRTILVCSWDGEEYALTGSTEWGEQFADELKKKLVAYLNVDESVAGAATTAGPEG
                     LSFSPSAVASLAPMLVEASKDLRAPSGKSLYEAWRATTMRDSKAGSAPFDSALVETRI
                     GSGSDHTVFLNHLGRPVINLGFTGDYGVYHSMYDDHYWMTHIGDPAFEYHVALTRIWG
                     IVALRLANADILPFDFGFNASALELFLRELEQKNKIEQRRLQLKRLHVRIAEFEKAGG
                     LLRGAVARDLLSGSAPPEKIQRLNEQLLRVESNWLDPAGIPGRPWFQHLLYSSRYTYA
                     HLEFPGLTEAVEKQDWKLAGEQAALLEKAVEKNTKLLRSAQSSWEKNKL"
     gene            6971..8167
                     /locus_tag="VGR03_07765"
     CDS             6971..8167
                     /locus_tag="VGR03_07765"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEV1994211.1"
                     /translation="MIRTICFRALGILLCSLAAISLAAAQDTPPAKPRARDLGVPFDG
                     TPGPLNAITDVFGVTVGHTTLISGEGKLQVGKGPVRTGVTAILPRGKDSMNNPVFAGW
                     WSLNGNGEMTGTTWVEESGFLEGPVMITNTHSVGVVRDAVIQWRVNHGQPDPTGYWWS
                     LPVVAETWDGWLNDINGFHVKSEHVFQAIDSAKSGRVEEGNVGGGTGMICDEFKGGIG
                     TSSRKVDTKIGEYTVGVLVQCNYGRRSDLRIAGVPVGREIPEDPAYASTSFSDSDRGS
                     IIVVVATDAPLVSHQLKRLARRVSLGLGRNGSISGNGSGDIFIAFSTANSGAAAADHV
                     VDLKMLPNDHLDPIFAATVQATEEAVINAMVAAETMTGIENHRVIALPHDKLREVLKK
                     YNRLAP"
     gene            complement(8232..9431)
                     /locus_tag="VGR03_07770"
     CDS             complement(8232..9431)
                     /locus_tag="VGR03_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEV1994212.1"
                     /translation="MPVGTAFHERTLALCESLNYREWSGYYTVSVYEMHHEHEYNAIR
                     NSAALIDISPLFKYRVTGQDATKFVNRVISRDINKVAVDQVIYCCWCDPEGKVIDDGT
                     ITRLGENEYRWTAADPSLRWFQQNALGLDVKIEDISEQTAALALQGPTSAKLLHAAAD
                     ANITNLKYFRVTHGKIAGVPVDISRTGYTGDLGFEVWMPWKDAVKVWDALIAKGKAFD
                     IHPAGMIALDIARIEAGLILIEVDYISSKKALIESQKYSPAEIGLGKLVDLKKENFVG
                     REALSQEAKKGAERALVGLEINWNEVEALYDKLKMAPQVPSMASRTPVPVYRGGRQVG
                     KATSTTWSPTLKKMIAIACVTREHSAVGTTLSMEMTVEAARHTVSAKVVPMPFFNPPR
                     RMSVPVT"
     gene            9695..10504
                     /locus_tag="VGR03_07775"
     CDS             9695..10504
                     /locus_tag="VGR03_07775"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEV1994213.1"
                     /translation="MQKTHLARTAVLYSLLLFSVSFCAAEDIKQIHPTGYVSDLAGVI
                     GPEARTRLEALCAEVERKTGAQMAIVTVQSLEGESVENYAVNLFKQLGVGSKKDNRSV
                     LLFVAPNERKYHIEVGYGLEPVINDARAGDAGRAMVPYLRQGDYGKAAEAGAWQLARY
                     IAADAGATLSGQPLAARNQNEGGNFGFPVFWVILGLIVLFRVIGSLSGGRRGGSGGSG
                     LLWFLLGMFANSSGGRSGSSWGGGGFGGGGGGGFGGFGGGSSGGGGASGSW"
     gene            10531..11283
                     /locus_tag="VGR03_07780"
     CDS             10531..11283
                     /locus_tag="VGR03_07780"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEV1994214.1"
                     /translation="MLSTDTKLTELVRRLKEFAAANLECVILFGSAARGDFHEGQSDL
                     NVVCILHSLSVEELGRLAHVVKWWCVEQKEPAPLFFTKDELKHAADVFAIEILDMKHG
                     RRVLYGGDLVAEIEVPMNLHRVQIEHDLRTIVLKLRQHYLRAPGNAKELAPILRKSFS
                     GVLTLLRHVVIAFGEEPPVAAHDIVARAAALTGSSTAAFGAMLKLRETGEFHGEIVPV
                     YGAYLKALEKTVDALEHHLPKREWQRAKNARS"
     gene            11306..11932
                     /locus_tag="VGR03_07785"
     CDS             11306..11932
                     /locus_tag="VGR03_07785"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV1994215.1"
                     /translation="MKKGLMVLVVLAVIALMLGSSFISRRNQMAVKREAVNAAWAQVD
                     VVLQRRADLIPNLVETVKGFAAQEQTVFGDIAKARSALLGAHSPAEKIAANGQLDSAL
                     GRLLVVVENYPQLRSNENFLRLQDELAGTENRIAVERRRYNETVQDYNTFIVLFPNSL
                     VASLGGFTRNDAYFKTEEGARQVPKVNFGTGNAPTAPATTPKPAPAKP"
     gene            complement(11962..12468)
                     /locus_tag="VGR03_07790"
     CDS             complement(11962..12468)
                     /locus_tag="VGR03_07790"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEV1994216.1"
                     /translation="MTGDDVSVIVLLMGPAGSGKTTVGELLAAQLSWTFFDGDNFHSP
                     ANIEKMSHGVPLGDEDRIPWLNSIREAMLLWDAQGRNAVLACSALKRSYRELLQINSN
                     VKLVYLKGSYELFRERLRSRKGHYAGEQILAGQFADLQEPSDAITIDAAQSPKQIVAE
                     IRKRLAIA"
     gene            complement(12465..13598)
                     /locus_tag="VGR03_07795"
     CDS             complement(12465..13598)
                     /locus_tag="VGR03_07795"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV1994217.1"
                     /translation="MITKPLHLDVHEQIECAQTLASRFYIDPAILEIEKTRIFQRTWQ
                     LAGTLSHACGEVNGAKRTISDPESFFIADVAGEPIIVVRDNHGELRAFSNVCRHRAGP
                     IALGSGCKNVLRCQYHGWTYTLDGRLIGTPDVEGVEFFDRSTMGMVPLRCETWEQFIF
                     VNFDLKAEPLSAYLGQIPQQARGFQFAGLQFAERRDYVIDCNWKIYVDNYLEGYHIPI
                     AHPGLMKEIDYAQYRTDTFRYYSQQFAPIRAMNAEDVGERLYAPGTGLQQALYFWVFP
                     NLMLNIYPDNISTNLIVPLSHEKTLTIFEWFFHDTASEKNHERIKRSVAFSDEVQQED
                     IGLCENVQRGLRSSTYDRGRYSVKRENGVHHFHMLLGEFLGQA"
     gene            complement(13642..15135)
                     /locus_tag="VGR03_07800"
     CDS             complement(13642..15135)
                     /locus_tag="VGR03_07800"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEV1994218.1"
                     /translation="MSSAVTTSVSELQNYVNGTWRRSAATEYADVTNPATAELLARTP
                     LSTSADVDAAVQAAADAFPAWRRTPAGERVQYLFRLKNLLEENLDELSRLITLENGKT
                     FGEAKAELRRGIENVEVACGIPMMMQGHVLEDVTPGVDEMQIRQPLGVVAAITPFNFP
                     AMIVYWFLPYAIACGNTFILKPSERVPLSMRRLFELLEKTGLPKGVVNLVNGGRDVVN
                     ALCDHPKVRAISFVGSTPVAKHVYTRSAASGKRMQCQGGAKNHVIVLPDADMDLATQI
                     ISDSAFGCAGQRCLAVSVAVTIGEAQKTFRDSIAHAASAIRVGNGLDAGVQMGPVITK
                     ESKQRIESLISAGVGEGAKPIVDGRNTKIPKYESGNFLTPTVLDGLPSTSKLADTEIF
                     GPVLSLVHANSIDEAMEFLRRSPYGNQASLFTTSGSAARKFRYEAPAGNIGINIGVAA
                     PMAYFPFSGWKESFFGILHGQGRDAVEFFTESKIVVERWSRQETRKF"
     gene            15741..16196
                     /locus_tag="VGR03_07805"
     CDS             15741..16196
                     /locus_tag="VGR03_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994219.1"
                     /translation="MTEKKIVAERRKAEEPEVGVSWHCGKEVCLEEAFLSIAGHPDLA
                     KPQSAGHVPTGSHESIGEKSRVPGGKQMDDLREQEARELVKVLRDSPANCGLLAMQGI
                     VVMRCPGAPYTDTPTRFNESVLHDAIALSLLEKHKASGSYKWEYYMAKK"
     gene            16240..17028
                     /locus_tag="VGR03_07810"
     CDS             16240..17028
                     /locus_tag="VGR03_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994220.1"
                     /translation="MKRISLSLACLIGLSAAPVWAQQNKAVSDDCKPVSLARESFGME
                     AKIQKVVGSSTSEVEKFAIWMAESALEQRMISCIETFGKAGGGLTQEDIDFILSELKN
                     LSFINDTASKSGFLGNQSGGFIPYTSQDPAEIEKKVPACRGVLALVPKIREFSLSHRD
                     TSALRLSEVVQFYPEADTISCAFEARKAGYRDVAFQVLSSHLNIEMLSGVARSNENKV
                     LSKALEALPLNTPFTVSIPRQAPLHCTATTTNLGSEKITEMNCY"
     gene            17277..17489
                     /locus_tag="VGR03_07815"
     CDS             17277..17489
                     /locus_tag="VGR03_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994221.1"
                     /translation="MPEWFFDVKSADELDAEIWNIFALDNVGRSDEIHGTVHVSYSDE
                     AVKQAHLVPALTARGVAFSWEGKLAK"
     gene            complement(17550..17939)
                     /locus_tag="VGR03_07820"
     CDS             complement(17550..17939)
                     /locus_tag="VGR03_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEV1994222.1"
                     /translation="MTEPIWIDERDALALHDRLLALDGGAVGLRDAGLLQSALSRPQQ
                     LHAYGDNPDIIDMAAAYIVGIVRNHPFIDGNKRTGFLLGVLFLELNGARFTATEESAA
                     QAILSLAAGTLDEPALAAWLRANAKRP"
     gene            complement(17958..18185)
                     /locus_tag="VGR03_07825"
     CDS             complement(17958..18185)
                     /locus_tag="VGR03_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEV1994223.1"
                     /translation="MLELKVRKIGNSLGVVLPKEAVSRLQAGEGDRLFLIEGPDGAYQ
                     LTPYDPAFEKKMNQAEDIIGRYRNTLHTLAK"
     gene            complement(18266..18796)
                     /locus_tag="VGR03_07830"
     CDS             complement(18266..18796)
                     /locus_tag="VGR03_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994224.1"
                     /translation="MAFLEEKFGDWKPVSRLTGIAWLCFYALFLLYALANRSGFLILD
                     YVNLIIHEGGHFFFSWFGNTIMILGGTLGELLVPLLCAIYFFWQREATGFAFSNFWFF
                     ENFPYIGTYMADARSAALPLVGSEESDWTILFSQWGLLAQDQKIGGIMRALGYLGMLA
                     TMAWLAYRLRQDAVSK"
     gene            complement(18885..19271)
                     /locus_tag="VGR03_07835"
     CDS             complement(18885..19271)
                     /locus_tag="VGR03_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994225.1"
                     /translation="MFIGAHVLLYSDDPEADRAFFRDVLEFPAVDIGGGWLIFALPPA
                     EAALHPSDGDNRQLHGGRHLLGAVLYLMCDDLHSLIKSLDVKNVSCSPVSEEAWGIKT
                     SIRLPSGGEIGLYQPKHATALGLGSN"
     gene            complement(19349..20332)
                     /gene="glk"
                     /locus_tag="VGR03_07840"
     CDS             complement(19349..20332)
                     /gene="glk"
                     /locus_tag="VGR03_07840"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEV1994226.1"
                     /translation="MILAGDVGGTKCNLALFSEKGGTLTPVFKQRFASKEFAQFERIV
                     KEFSRQAAEHLGKESIVAAGFGIAGPVINGTVRATNLPWIVDTKTLIKELNVPHVVLL
                     NDLGATGHSIEHLPPEDFCVLNPGNPEPGGTRALLAAGTGLGQSILVWDGGRYRVVPS
                     EGGHSDFAPHTEQQIELLRFMRRRYPQVSWELILSGRGFRTLHEFLAPKVIHPSFDDP
                     DADPAPEITRRGLDKSCRVCSDTLDLWTAIYGAEAGNLALKVLALGGVYVAGGIAVKI
                     LEKMKDGTFFHAFKDKWKFEGLLSNIPVSIVLNESAPLLGAAYEALAAIDK"
     gene            complement(20383..>20900)
                     /locus_tag="VGR03_07845"
     CDS             complement(20383..>20900)
                     /locus_tag="VGR03_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994227.1"
                     /translation="FVAGKLLIDNWRWAGVPFYLRTGKRLAKRTTEIMIQFRRAPHIV
                     FREKEIMPNRLILNIQPDEGISISFGAKRPGTEMSIGNVTMNFCYREAFGEGTRSAYA
                     TLLNDCVRGDATLFDRGDSVEAAWSLVDPILDVWSAAKSATVPQYSAGSWGPRESDVL
                     LERDGRQWYNP"
BASE COUNT         4391 a         5843 c         6141 g         4525 t
ORIGIN      
        1 ccccaggaag ccgccgaaaa aaataatgta gaaccaatag ttctccggcc ggcgccgcac
       61 gaagtgcacg atcgccaggc cctgcacgat gaagccccac ggatagaaga ggtagctaag
      121 tccaaacatg gatgaccgcc ctgtcaacag aatctcttgg cttttcaggc gggtcaaacg
      181 gaaactccgc cccgtgcatg cgagagtaac tgcccgttgg aacgattccc gcctgctatg
      241 atggtccctc atgaacccca tcgaaaccct cggcctcgcg ctaggcgccg gattttcttc
      301 cggcctcaat ttctacgcca ccgtcgccac ccttgggttt ctccaccgct tcggcgtgct
      361 cagcctgccg cctgggttgc aggttctttc tcatccctgg gttttgggaa tcgccatcgc
      421 gctctatctg ctggaattcc tcgcggacaa aatcccgtac ttcgacacca tctgggacgt
      481 catacatacc ttcatccgcc cgcccgccgc cgcgcttctt gcttttgccg ccgctggagg
      541 cgccacaccc gaatggcgct ggggcgccgc gttgattgcc ggcggagtcg ccctcaccgc
      601 gcacggcacg aaagccagcg cacgcgccgc cgtgaatgcc agtcccgagc cgttcagcaa
      661 ttgggcgttg agtattggag aagacgtctt ggccgtgtgg ctcgcgtgga tggccacggc
      721 tcatccgctc gcgacaacga ttgtcgtcgc cgcactcgtc gcgctctcag ccttcttgct
      781 ctaccatctc ttccgcttcg cgcgccgcgc tcttcagcgc ctgctcgctt cttgatctct
      841 ttgtacgggc ccccgccgct ccttttggcg acttgaatct tttgtagagg caaacttcag
      901 ttcgcactgt tttgtggttg taggggcgca gcactgctgc gccctctttg ggcaaggctg
      961 ttccgctact tcacgtacgt cgacagccaa tccaaaaacg tcttgtacca gaactggctg
     1021 ttctgcggtt tcagcaccca gtgcccctca tcggggaaca ccaccagctt cgacggcaca
     1081 ccctgccgct gcagcgcgct gaacaattcc agcgattgcg tgtacggcac gcgataatcg
     1141 cgctcgccgg caatcaccag cgtcggcgtc ttgaattttc ccagctcgcc cgcatacgtc
     1201 gagggcgacc acttcttgta gttctccggg ctcgtccacg gcgcgccctg catgtcgtgc
     1261 tcttcaaacc aaagttcttc cgtcgcgaac atcgaaacct tgtcgtagac gctcgcgtgg
     1321 ctcacgatgg ccttgaaccg tcccgtgtgc gtcgcaatcc aatccgccat gtacccgccg
     1381 taagaaccgc ccgccgccgc cattcgcgtc ccatcgatga aagcatattt tttcaacgtg
     1441 tagtccacgc cattcatcac gtcgacgtag gctctcccgc cccagtcatt cgtaatctca
     1501 tccgtgaatt tctgcccata ccccgtcgaa ccgcgccggt tgatcatcag cgtcacatat
     1561 cccgccgcgg agaatacttg cgcgttccac cggtaccccc acgcattcga ccacatcgtt
     1621 tgcggcccac cgtgcagcag caccaggagg gggtatttct tagtcgcatc aaatctcggc
     1681 ggccgaatca gcatcgcttg tactttcgtg ccttccgcgc cgtcgaacca aaacgtctcc
     1741 ggcgcgttca tttccactga agaaagaatt gaatcattct gatgcgtgag ttgccgcacc
     1801 ttggatccgt cgctcgatgc tgcaaatagt tccgctggaa gtgtcaggct cgtccgttca
     1861 aaaacaagcg tcttgccgtc cgcgctaaac gaaatcgccg tgttgtaggt atcggcgata
     1921 attttcttcg gctccgcgcc aaaacgcgcc gccatcgcgt acaccggctt ctgcgtttcg
     1981 ttctccgctg tgaaataaat cgtcttgctg tccggcgacc aggccagttc ttccgcgctg
     2041 cagtcaaacg tttccgtaag attttcgatt cttcccgccg cgcggtcgta cagcagtacg
     2101 cgccagcggt ccgcttcata ttccgcggta agctgcgcgt gatacgcgat gaattttccg
     2161 tcgggcgaat acactgggtt gccgtcaaat cccggctgcg tggtgatgcg cttggcctcg
     2221 ccgccggcaa ccggaacgat gaacaaatca ccgttcgtgc tgatcgcttc catcttgtcc
     2281 gtcaccgccg tgaaacacag ttcttttcca tccggcgaaa aactgatatc gcccggtccg
     2341 ctccgctcat ccggtggaac atcgtaattt gctcccggcg tgaggtctcg cggtgccgcg
     2401 tttgcgtccg ctggaaccac aaacaaatgg cccctcttgc catcattcca atgcgtccag
     2461 tggcggtaca gaagctgttc cgccacatgc gccttcactt tgctcttctc tttttctgcg
     2521 tcgcgcttac tgttgcaatc gtcgtctttg caatccggat acacggaaga agtaaacgca
     2581 atcgtctttc cgtccggcga ccacttcacc atatccgcgc cggtcgagag tttcgtcagc
     2641 gggtgcgctt cacctccatc catcgaaagc aaatacacct gcgaatcgcc gctgcgcgag
     2701 gagagaaacg cgagcgtctt tccgtccggc gaccacaccg gcgacgaatc atgcccgctc
     2761 tgagtaagtt gagtcacgga accgcccgtc gttggcacca tccagatgtt gctcgcatta
     2821 cggtttgcat ccatgtccgg cgtcgccacc gtgtacgcca cccactttcc atccggcgac
     2881 acttgcggct ccgcaatgcg gtgcaacttg atcaggtcgt caaaagtgat gggatgtttc
     2941 gccgattctt gtgcagtaat cgcaaatcca aatcccagca gcaacgcgcc caaaagaccg
     3001 caccgcaaaa tgacgccttt catttcgatt cttctcctgt gaaacaagat gagtcgcaac
     3061 gctccttgca gaatcaggcc atgcagaatt acgctttgca gaattacgga tgatgtcacg
     3121 tttccctgcc gaatccaata gtccggcccg ccagcctcgc ggaattgtga cgcgacttcc
     3181 gccatgcacg ccgcgatgac cgcgatcccc gcaaacgcca ccagaaaacc gatgggactc
     3241 catcgcccga gccgtcggat agttcgaaac tgaccaactt cttattggct ggtacgagca
     3301 attttcgttc acaggctttc gcgcgtcttg ctgcgcgcac ttgcgactct gctacaatcc
     3361 tttgccggac atctcatgcc gacgagcagc ggacttttcc cccgcaattt caagaagacc
     3421 tttcctattg ccattcgcgg ggaaggttgc tggatcgtcg ctgacgacgg gcgccgattt
     3481 ctcgatgctt ccgggcaggc cgcggtcgtc agcatcggtc acggtgttgc tgaaatcggc
     3541 cgcgcgatgg ccgagcaatc gtcgcagatt gctttcgcgc acacttcgca atttcacacc
     3601 gctccagcgg aacaacttgc cgcgcgcttg ctcgcgatgg ctccgccgaa tttccgtaac
     3661 ggcggccgcg tgtattttac ttctggtggc tctgaagcta ccgaaaccgc cataaaactc
     3721 gcacgccagt ttcatcttga aactggccag aatgatcgct accgcgttct ctcccgccgc
     3781 cagagttatc acggcagcac tcttggcgcg atgtccgtca gcggcaatgt cgctcgccgc
     3841 gcgtcttacg aaccgctcct cgcggaatgg ggtcacattg ctccgtgttt ttgttaccac
     3901 tgcccgtacg aaaaagcttt tccgcaatgc gaactcgctt gcgctgacga tctcgaagtc
     3961 cacctgctcg aacacgaagc cggtcaggcc gccgctttta tctttgagcc ggtcgttggc
     4021 gctactctcg gcgccgccgt gcctcccgaa ggttacgcgg cacgcatcgc ggaaatctgc
     4081 cgcaagaacg gaattctcct gatcgccgat gaggttatga gcggcatggg ccgcacgaga
     4141 aaaccgttcg ccgtccagca ctggggcatt gagccggaca tgattctagt ggggaaagga
     4201 atcgcgagcg gttacgcacc gcttggcgca gtgcttgttt ccgcgcgcgt cgtcgaggca
     4261 ttcgaacgcg gctccggagc cttcatgcac ggcttcacgt atcaggcgca tcctgtttgt
     4321 accgccgctg gcaacgccgt tctcgactac ctcgaatcgc acaagctttt tgatcgcgtg
     4381 tataccgccg ggcagagcct gctcggcgcc ctttctccgc tgcaatcgca tccgaacgtc
     4441 ggcgatgttc gcggtctcgg tcttcttctc agcgtggagt tcgtcaaaga caaagccacg
     4501 cgcgagcctt ttccaaaaga acaaaacatc gccgagagaa ttcgccacgc ctgtctggaa
     4561 gaaagcgtgc tgacctatcc gacgcaaggc tgtgttgacg gtttgcgcgg cgaccatatc
     4621 cttctcgcgc cgcccttcac gatctcgcgt gaggaatgcg cgctgatcgc tcgcgcgctc
     4681 cagtcggcgg tgacaaaagt gttttcggtt tgattcggca ccaggtcaag attgttcgtt
     4741 gcaatacggt tgctcttctt ttggagaaca aaataaaaat gcggcttcct ttgcgtactc
     4801 ttgcggtctc cttcatttct ttgctgattt tgaccttcgc gctttctgcc cagctcaagc
     4861 aagatgctcc gccctcgtct ccgattctcg gtttttcccc gccgcacgcc gccgcggagc
     4921 acgaagtcga atccacgttt cagtcgattc cgtctcccga aaaagctcgc gaatggcacc
     4981 gcaccttcac cgccgaaccg caccccgcgg cttccgaccg caataatcag ctcgccgatt
     5041 ttgtcgcgga ggaatggcgc aagcagggat gggaagacgt cacgcttcgc cgctacgatg
     5101 ttttgcactc gcgcccacgc agcgtttctc tcgaaatgat cgagccggtc cactttacag
     5161 cttcactgcg tgaagatgct tacgacgtcg atcccgatac caagaatccc gctgtctccg
     5221 gctcctactt tggctattcc gcatcgggcg acgtcactgc cgaagtcgtc tacgcgcaca
     5281 gcgggaatcc ggaagattac gatttgcttc ggaaaaacgg cattagcgtg aaagggaaga
     5341 tcgttctcgt ccgctattcg aatccttaca gctatcgcgg cttcaaggcg ctcactgccg
     5401 agcgcgaagg cgcggctgcc ctgctcatct attccgatcc cgctgaagat ggttcgacgc
     5461 gcggcaaagt ttttcccgac ggcccgtggg gcccggaaag tcacattcag cggggcgcga
     5521 ttacttacga ttacatcgtg cccggcgatc ccaccacgcc cggctgggct tccgtcgcgg
     5581 gcgcaaagca catcgctccg gaagcagcgc tctcgctccc gaagattcct gcgctccccc
     5641 tctcgtggcg cgacgcgaaa ccgcttctcg aaaacatgaa cggccaagaa gcgccgaggg
     5701 attggcaggg tgctctgccc atcacgtatc gattcagcgg cgcggtgaaa gttcacgtga
     5761 aagtggacat ggacaccagc atgcagcctt acacggtcgt cgaagcgcgc attcgcggca
     5821 gcgagcttcc cgacgaatgg gttctgctgg gcaatcatcg cgacgcttgg gccttcggcg
     5881 gcgtggatcc ttccagcggc accgcctcga tgatggagct cacgcgttct cttggcgaac
     5941 tcaagcagcg cgggatccgt ccgcgccgca ccatcttggt ttgcagttgg gatggggaag
     6001 aatacgcgct tacggggtcg acggaatggg gagagcagtt cgccgatgag ttgaagaaga
     6061 aactcgtcgc gtatctcaac gtggatgaat ccgtcgccgg agccgctact actgctggtc
     6121 ccgagggctt gagcttctcg ccttccgcag tggcttctct cgcgccgatg ctggtggaag
     6181 cttccaaaga ccttcgcgcg ccttcgggaa aatctctgta cgaagcgtgg cgagctacga
     6241 cgatgcgcga ttcgaaagcg ggcagtgcgc cattcgacag cgcgctcgtg gaaacgcgca
     6301 ttggcagcgg ctccgatcac acggtgtttc tgaatcacct cggccggccg gtcatcaacc
     6361 tgggtttcac gggcgactac ggcgtgtatc attcgatgta cgacgaccac tactggatga
     6421 cccacatcgg cgatccggcg ttcgaatacc acgtcgcgct gacgcggatc tgggggatcg
     6481 tggcgctccg tctcgcgaac gcggacattc tgccgtttga cttcggcttc aacgcgtcgg
     6541 cacttgaact gttcctgcgc gagctcgagc aaaaaaacaa aatagagcaa cggcgtctcc
     6601 aattgaaacg gcttcacgtt cgcattgccg aattcgaaaa agcgggcggc cttctgcgcg
     6661 gcgcggttgc tcgcgatctt ctgtcaggct cggcgccgcc ggagaaaatt cagcgcctca
     6721 acgagcaact gctgcgggtt gaatccaact ggctcgatcc tgcgggcatc cccggccggc
     6781 cgtggttcca gcatcttctc tattcttcgc gatacaccta cgcgcacttg gaatttcccg
     6841 ggctcacgga agccgtagaa aagcaggatt ggaaactggc gggcgaacaa gcggcgctgc
     6901 tcgaaaaggc cgtggagaaa aacaccaaat tgctgcgctc cgcgcaatca tcctgggaga
     6961 agaacaagct atgattcgta caatttgttt tcgcgctctc ggcatccttc tttgttcgct
     7021 cgcggcaata tctttggcag cggctcaaga tactccgcct gccaagccgc gcgcccgcga
     7081 tcttggcgtc ccttttgatg gaacgcctgg cccgctcaac gccatcaccg atgttttcgg
     7141 cgtcaccgtt gggcacacga cgctcatttc cggtgaagga aagctgcaag taggtaaagg
     7201 gcccgtccgc accggcgtta ccgcgatcct tccgcgtgga aaagattcga tgaacaatcc
     7261 ggttttcgcc ggctggtggt cgctgaacgg taatggcgag atgaccggca cgacctgggt
     7321 cgaagaatcc ggattccttg aagggcccgt gatgatcacc aacacgcaca gcgtcggagt
     7381 ggtgcgggac gccgtcattc aatggcgcgt caaccatggg cagcccgatc ccaccggtta
     7441 ctggtggtcg ctgcctgtcg tcgcggagac ctgggatggc tggctcaacg atatcaacgg
     7501 cttccatgtg aagtccgagc atgtgtttca agccatcgat tccgcgaagt ccggccgcgt
     7561 tgaagaaggc aatgtcggcg gcggcacggg aatgatctgc gatgagttta agggcggcat
     7621 tggcacatcc tcgcgcaagg tagacacgaa aataggcgag tatacagtcg gcgttctggt
     7681 gcaatgcaat tacggacggc gctccgattt acgcatcgct ggagtaccgg tgggccgcga
     7741 aattcccgaa gaccccgcgt acgcttccac gtctttcagc gattcggacc gcggctccat
     7801 catcgtcgtg gtcgccacgg atgcgccgct cgtttcacat caactgaagc gtctggctcg
     7861 ccgcgtttct ctggggctcg gacgcaacgg cagcatctcc ggaaacggtt ccggcgatat
     7921 tttcatcgcc ttctccaccg ccaactccgg cgctgccgct gccgaccacg tcgtggattt
     7981 gaaaatgctt ccgaacgatc atctggatcc catctttgcc gcgacggtgc aggcgaccga
     8041 agaggcggtg attaacgcca tggtcgccgc cgaaactatg accggcatcg aaaatcacag
     8101 agtaatcgcg ttgccgcacg acaaactccg tgaagtgctc aagaaataca atcggctcgc
     8161 gccatgatgc ccactgagtt cttgaattgg ctttgtgatt gaacgacgaa gaaaacgcga
     8221 ttgcgcgcgt gtcaggtcac cggcactgac atccttcgcg gtgggttgaa gaagggcatc
     8281 ggtacgactt ttgcggatac ggtgtggcgt gctgcctcta ccgtcatttc catgctgagc
     8341 gtggtgccga ctgcggaatg ctcgcgagtt acgcaggcga tcgcgatcat ttttttcagc
     8401 gtcggtgacc acgtcgtcga ggtggccttg cccacttggc gcccgccgcg atatacgggc
     8461 accggggtgc gcgaggccat gctgggcacc tgcggcgcca tcttcaactt gtcgtagagc
     8521 gcctcgacct cgttccaatt aatctccagc cccacgaggg cccgctccgc gcctttctta
     8581 gcttcttgtg acagcgcctc gcgccccacg aaattttctt tcttcaggtc cacgagcttg
     8641 cccagtccga tctcggccgg cgaatacttc tgcgactcga tgagcgcctt cttgcttgag
     8701 atgtagtcca cctcgatcaa gatcaggcct gcttcgattc gcgcgatgtc cagcgcgatc
     8761 atacccgcgg gatgaatatc aaatgctttg cccttggcga tcagcgcatc ccacactttg
     8821 accgcgtcct tccacggcat ccacacttcg aatcctaaat cgccggtgta tccggtacgc
     8881 gaaatgtcca cgggcacgcc cgcaatcttt ccatgcgtca cgcgaaaata cttcaggttc
     8941 gtgatgttgg cgtccgcggc cgcgtgcagc agtttcgcgg acgtcggccc ctgcagtgcc
     9001 agcgccgccg tctgctcgga aatatcttca atctttacat ccaaacccag tgcgttctgc
     9061 tgaaaccagc gcagacttgg atctgccgcc gtccagcgat actcgttctc gcccagccgc
     9121 gtgatcgttc cgtcatcgat cacttttcct tccggatcgc accagcagca gtagatcacc
     9181 tgatccaccg caactttatt gatgtcgcgc gaaatcacgc ggttcacgaa cttcgtggcg
     9241 tcttggccgg tcacccgata tttgaagagc ggtgaaatat cgatcagcgc cgcggaattg
     9301 cggatggcgt tgtactcgtg ctcgtgatgc atctcgtaaa cgctgaccgt gtaatagccc
     9361 gaccattcgc ggtagttcag gctctcgcag agcgccagcg ttctctcgtg aaacgcagtt
     9421 cctaccggca aggatcctcc tgatgcgcac gcggctgacg acgccgcaaa gacactggat
     9481 tataggtgcg aactcgccac gctcgcaagg ttcgcttcac agaagataga ataaagatgg
     9541 agagcgccgg tcctgaatac ttgcattcct gtgaccgcga tcactgtttt ccttcgcaaa
     9601 aatgaggtac ctagaccctg gagaaggctg acgctccttt acacctggcc gaagccggcc
     9661 ctatagtcat ctggcgatcg ccttcggaca ctgcatgcaa aaaacccacc tcgctcggac
     9721 cgcagtgctt tacagcctgc ttttgttcag cgtgagtttc tgcgcagcgg aagacatcaa
     9781 acaaattcat cccaccggct atgtgagtga cctggccgga gttatcggcc cggaagccag
     9841 gacacggctt gaagcgttat gcgcggaagt tgaacggaag actggcgcgc agatggcgat
     9901 tgtgaccgtc caatcgctcg agggcgaaag cgtcgagaac tatgctgtga acctcttcaa
     9961 gcagcttgga gtcggaagta aaaaagacaa tcgcagcgtg ttactgtttg tcgctcccaa
    10021 tgagcgaaag tatcacatcg aagtgggcta cggtctcgag ccggtgatca atgatgcgcg
    10081 ggccggagat gcgggtcgcg cgatggtgcc ctatttgcgc cagggtgact acggcaaggc
    10141 ggccgaggct ggcgcttggc agttggccag gtacatcgcg gccgatgctg gcgctacctt
    10201 gagtggccag ccactcgcgg cgcgaaatca gaacgagggt ggaaactttg ggttcccggt
    10261 attctgggtc attctgggtt tgattgtttt gtttcgtgta attggcagtc tctctggcgg
    10321 ccgtagaggt ggctcgggtg gcagcggctt gctctggttt ctgctgggga tgtttgcgaa
    10381 cagctcggga ggtcggtcgg gaagcagctg gggaggcggc ggcttcggcg gaggcggcgg
    10441 tggtgggttt ggaggatttg gcggcggcag cagcggaggc ggaggcgctt ccggaagctg
    10501 gtagcgcggg atgaaaggaa tggatgcgaa atgctctcca cggacacaaa actgactgaa
    10561 ttggtaagga ggctgaagga attcgcggcg gcaaacctcg aatgcgtcat tttgttcggg
    10621 tcggctgcgc gcggggactt tcacgaagga cagtcggacc tcaatgtggt ttgcatcctt
    10681 cattcgttga gtgtcgagga gttgggacgc ctcgcacacg tggtgaaatg gtggtgcgtc
    10741 gagcagaaag agcctgcgcc tctcttcttc acgaaagatg agctgaagca tgccgcggat
    10801 gtcttcgcca tcgagattct ggatatgaag cacgggcggc gagtgctcta cggaggggat
    10861 ctcgtcgcgg agattgaagt gccgatgaac ctgcatcgcg tgcagattga gcatgacttg
    10921 cgcacgatcg tgctgaaatt gcggcagcac tatctgcgcg cgccgggaaa tgcgaaggaa
    10981 ctggcgccca tcctgagaaa atccttttcg ggagtgctga cgctgctgcg ccatgtggtg
    11041 attgcttttg gagaagagcc gccggtggcc gcgcatgata tcgttgcgcg agcggcggcg
    11101 ctgacgggct caagcacggc ggcattcggc gcaatgttga aactgcgcga gacgggcgaa
    11161 tttcacggcg aaattgttcc ggtgtacggt gcgtatctaa aggcattgga gaaaaccgtg
    11221 gatgcgctgg agcatcacct tccaaagcgg gaatggcagc gcgcgaagaa tgcacgttcg
    11281 taaatccaac tgattcgagg cggatatgaa gaaaggtctg atggttttgg ttgttttagc
    11341 ggtcatagcg ctgatgctgg ggagttcgtt tatcagccgc aggaatcaga tggccgtgaa
    11401 gcgtgaggcg gtgaatgccg cttgggcgca ggtggacgtc gtcctgcaac ggcgagcgga
    11461 cttgatcccc aatctggtgg agacggtgaa gggctttgcg gcgcaggagc aaaccgtgtt
    11521 cggcgatatc gccaaggcgc gttccgccct gcttggcgcg cattctccgg ctgaaaaaat
    11581 tgcggcgaat ggacaattgg attctgcttt agggcgcttg ctcgtagtgg ttgaaaacta
    11641 tccccagctg agatccaacg aaaacttttt gcgtctgcag gacgagctgg caggcacgga
    11701 aaatcgcatc gccgtggagc gccgccgata caatgagaca gtgcaggatt acaacacgtt
    11761 tattgtgctc ttcccgaaca gcttggtggc gagcctgggc ggcttcacgc gtaacgacgc
    11821 gtacttcaag acggaagaag gcgcccggca ggtccccaaa gtgaatttcg gaaccggcaa
    11881 cgcgccaact gcaccggcta cgacacccaa gccagcgcct gcaaagccct aaagatctgc
    11941 tcctgcaagt ggcggcgcat cttaagctat tgcgagtcgt ttgcggattt ccgcaacgat
    12001 ttgttttggg gattgcgcgg cgtcgatcgt aatggcgtcc gaaggttcct ggagatccgc
    12061 gaattggccg gcgaggattt gctcgccggc gtagtgcccc ttacgcgaac gcaaccgctc
    12121 gcgaaacagt tcgtaggaac ctttcaagta gacgagcttt acattggaat taatttggag
    12181 cagctcgcgg tagctgcgct tcagcgcgga gcatgccaga accgcattcc ggccttgcgc
    12241 atcccacagc aacatcgctt cgcgaatgga attgagccac gggatgcggt cctcatcgcc
    12301 gagcggaacg ccgtgagaca ttttctcgat gttggcgggc gagtgaaaat tgtcgccatc
    12361 aaaaaaagtc cacgagagct gcgcggcgag cagttcgccg acggtcgttt ttccagatcc
    12421 cgctgggccc atcagaagaa cgatcacgga aacgtcatcg ccggtcacgc ctgccccaga
    12481 aactcgccga gcagcatgtg gaaatggtga acgccgtttt cacgcttgac ggagtagcgt
    12541 ccgcggtcat aggtcgaaga gcgcaaacca cgctgaacat tttcacacag gccaatatct
    12601 tcctgctgca cctcatcgct gaacgccacc gagcgcttga tgcgctcgtg attcttctcc
    12661 gaagccgtgt cgtggaaaaa ccattcgaag atcgtcagcg ttttttcgtg cgagagaggc
    12721 acgatcagat tggtcgagat gttgtccgga taaatgttga gcattaggtt cgggaatacc
    12781 cagaagtaga gagcttgctg caggcccgtg ccgggcgcgt aaaggcgctc gcccacgtct
    12841 tccgcattca tcgcacggat cggcgcgaat tgttgagagt agtagcgaaa ggtgtcggtg
    12901 cgatattgcg cgtaatcgat ttctttcatc aagccaggat gcgcgatggg gatgtgatag
    12961 ccctcgaggt agttgtcgac gtagatcttc cagttgcaat cgatgacgta gtcgcggcgc
    13021 tcggcaaatt gcaggcctgc gaactggaaa ccgcgcgcct gctggggaat ctgaccgaga
    13081 taggccgaga gcggttcggc tttcaaatcg aaattcacaa agatgaactg ttcccacgtc
    13141 tcgcagcgaa gcggcaccat gcccatggtg ctgcggtcga agaactcgac gccctcgacg
    13201 tcgggcgtgc cgatcaagcg gccatccagc gtgtaggtcc agccgtgata ttggcaacgc
    13261 aggacatttt tgcagccgct gccaagcgca atcggtcccg cgcgatggcg gcaaacgttg
    13321 ctgaaggcgc gcaactcacc gtggttgtcg cgaacaacga tgatcggctc gccagccaca
    13381 tcggcaatga aaaaactttc gggatcggag atggtccgct tcgcgccgtt cacttccccg
    13441 caggcatggc tgagagttcc ggcgagttgc caggtgcgct gaaaaatgcg ggtcttctcg
    13501 atttccagaa tcgcgggatc aatgtaaaaa cgcgaggcga gtgtttgggc acactcgatc
    13561 tgctcgtgca cgtccaggtg aagcggtttt gtgatcatcg aaagagagtc ctagcaaatc
    13621 aatagcagcg ctttgtaagt tctaaaactt acgcgtttcc tggcgtgacc agcgctcgac
    13681 tacgattttc gattcggtga agaattcgac ggcgtcgcgt ccctggccgt gaaggatgcc
    13741 gaagaagctt tctttccagc cgctgaaggg gaaataggcc atcggcgcgg cgacaccgat
    13801 gttgatgccg atatttcctg cgggcgcttc gtagcggaat ttgcgcgcgg cggagccgct
    13861 cgtggtaaag agcgatgcct ggttgccgta aggactgcgg cgaaggaatt ccatggcttc
    13921 gtcaatggaa ttcgcgtgca ccaaactgag tacagggccg aagatttccg tatcggcgag
    13981 cttgctggtg gacggcaagc catccagaac cgtaggggtg aggaaattcc ctgattcgta
    14041 ttttgggatt ttcgtgttgc ggccatcgac gatgggtttt gcgccttcgc caacgcctgc
    14101 ggagatcagc gattcgatgc gttgtttgct ttccttggtg attaccgggc ccatctgaac
    14161 accagcatcg aggccattgc cgacgcgaat tgcagaggcg gcgtgagcga tggaatcgcg
    14221 gaaggttttt tgcgcttcgc caatcgtgac agcaacagag acggcgaggc agcgctggcc
    14281 ggcgcagcca aacgcgctgt cgctgatgat ctgcgtggcg aggtccatat cggcgtctgg
    14341 caaaacgatg acgtgattct ttgcgccgcc ctggcattgc atgcgcttgc cgctggctgc
    14401 ggagcgtgtg taaacatgtt tggcgacggg ggtggagccg acaaaactga tggcgcgaac
    14461 tttcggatgg tcacacaacg cgttgaccac atcgcggcct ccattgacga ggttcacgac
    14521 tcctttgggc aggccggttt tctcaaggag ttcaaagaga cggcgcattg agaggggaac
    14581 gcgctcggat ggtttcagga tgaatgtatt tccgcaagcg atggcgtagg gcaagaacca
    14641 gtagacgatc atggcgggaa aattgaaggg cgtgatcgcg gcgacgacac cgagcggctg
    14701 acgaatctgc atctcatcca cgccgggcgt cacgtcctcg aggacgtggc cctgcatcat
    14761 catggggatg ccgcaggcaa cttccacgtt ttcgatgccg cggcgcagtt ccgctttggc
    14821 ttcgccaaag gtttttccat tctcaagagt gatgaggcgc gagagttcgt cgagattttc
    14881 ttcgagcaag tttttcaacc tgaagaggta ttgaacgcgc tcgccggcag gagtgcggcg
    14941 ccaggccgga aaggcatcgg cagcggcctg gacggccgcg tcgacatccg cggatgtcga
    15001 gaggggcgta cgcgccagaa gttcggcagt ggctgggttg gtgacgtccg cgtattcggt
    15061 cgcggcggat cgccgccagg tgccattcac ataattctga agctcggaaa ccgaggtggt
    15121 tacggcactg ctcatactta gctccttggc ctgcgcatgg aaagggggat gtattccctc
    15181 aggccagatc tacgaagaat cggatcgata tagtttacca agaaaccgta aggatgagag
    15241 gcgaatcgcg ggcagtgcgg gctctcaacc cgcgttgagc gacgacgaag gcgatagtgg
    15301 gtgccgcgtg gcgaacggga attcgtcgtc gcatggttca ttttgcttct cgctgaagtg
    15361 gagggggtac ccccaccccg ggtggttttt ggcaaagagt gcggaaacga ttgaaaataa
    15421 aagagttgag tttttggcgg gtgcaaaaga gtgcacaaga gtgtgaaaag aaaagagata
    15481 ggccaaaaaa atgttggccg gttggaaatg tttggcgggt ttgaagatgc ccactccccc
    15541 ctatttgtga aacgatatga atccaaaagg gttagagggt gggggtctgc aaacgatatg
    15601 attcgatgag acttagaata aggaagcagg gagttagctg aataacggag gcctcgacga
    15661 ggatgatggc tgacgatcac aactcatgtt aacacgaaag taccgtaatt gtcaagataa
    15721 atctggggcc ttggaaatga ttgacggaga agaagatagt cgcagaacgg cggaaggctg
    15781 aagagccgga agttggtgtt tcgtggcatt gcggaaagga ggtgtgtctt gaagaggctt
    15841 tcctttccat cgcggggcac cccgatcttg ccaaaccaca gagtgcgggc catgtgccca
    15901 ccggcagtca cgagtctatc ggcgagaaat caagagtgcc cggaggaaag cagatggatg
    15961 accttaggga acaagaggct cgcgaattag taaaggttct aagggactct cctgcgaatt
    16021 gcgggctatt agccatgcag ggaatcgttg tgatgcggtg tcccggcgcg ccctataccg
    16081 atacgcctac gaggtttaat gagtccgttt tacacgacgc cattgcattg agccttctcg
    16141 aaaaacataa ggccagcggc agctataagt gggaatatta catggcaaag aaataagacg
    16201 cgaagtaaat tcgggtgtac gtgtgccaaa gaggaacaaa tgaaaagaat atctctatcc
    16261 ctcgcatgtc tcattggttt atcggctgcg cctgtatggg cgcagcaaaa caaggccgtc
    16321 tcagacgatt gcaagcctgt gagtttggca agagagtctt tcggcatgga agccaaaatt
    16381 caaaaggttg tggggagttc cacgtccgaa gttgagaagt ttgccatctg gatggcagaa
    16441 agcgcattgg aacagagaat gatttcctgc atagagacgt tcggtaaggc tggcggcggt
    16501 ctaacacagg aggatatcga cttcattctc tctgaattga aaaacctgtc gttcatcaat
    16561 gacaccgcgt cgaagagcgg tttccttggc aatcaatctg gcgggttcat tccatataca
    16621 agtcaagacc ccgccgaaat tgagaaaaaa gttcccgctt gcaggggcgt tttggcactg
    16681 gttccgaaaa tacgagagtt ctcgctttcc catagggaca cttctgctct gcgactcagc
    16741 gaggtggtgc agttctatcc cgaggccgat acaatttcct gtgccttcga ggctcgaaaa
    16801 gctgggtaca gggatgtggc gtttcaggtt ctctcttctc acctaaacat agaaatgttg
    16861 tccggagtcg cccgctccaa tgagaacaag gtactctcta aagcgttgga agcgctgccg
    16921 ctaaacacac cattcaccgt atccattccg aggcaagcac cgctgcattg cactgcaacg
    16981 acaacgaatc tagggtctga aaaaataaca gagatgaatt gctactgagt tgaattgaaa
    17041 atgctagtaa gggtgcagat atgggagaaa tgactgcatg gcgataatct atttaaggaa
    17101 cccaatcaca aaagccgtgt acggcaccat catggccgtt ctcggattgg tggcgctgga
    17161 ttctaagccg tggataggct tgttgctctt gggcgtttcg gcattggtct tttggaacgc
    17221 agcccaacag tggaagtctc acacgaataa ttctccgcgc gactaggagg tgaaagatgc
    17281 ctgaatggtt tttcgacgtt aagagtgcgg acgaacttga cgcggaaata tggaacattt
    17341 ttgctctcga caatgtgggg cgcagcgacg agattcacgg gaccgttcac gtttcgtatt
    17401 cagacgaagc cgtgaagcag gcgcatttgg taccggctct tacggcacgc ggcgttgcat
    17461 tctcttggga aggaaagctt gcgaagtgaa atgggcgcac tgatgttgca aagtgagcta
    17521 gtacgcgtcg gacaccagaa gacgggcgat cacgggcgct tggcgttggc gcggagccag
    17581 gcggcgaggg cgggctcatc gagggttccg gcggcgaggc tgaggatggc ttgggcggcg
    17641 gattcttcgg tggcggtgaa gcgcgcgccg ttcaattcga ggaagaggac gccgaggagg
    17701 aagccggtgc gtttgttgcc atcgatgaag gggtggttac ggacgatgcc gacgatgtag
    17761 gcggcagcca tgtctatgat gtccgggttg tcgccatagg cgtggagttg ctggggccgg
    17821 gaaagtgcgg attgaagcaa gcccgcatcg cgcaggccca cagcgccgcc gtcgagggcg
    17881 agaagacggt cgtggagcgc gagggcatcg cgctcgtcga tccagattgg ttcagtcact
    17941 tggccagacc attccgctca cttggcgagg gtgtgcaagg tgttgcggta gcggccaatg
    18001 atatcctcgg cctgattcat tttcttttcg aaggcgggat cgtagggtgt tagttgatag
    18061 gcaccatccg gtccttcgat cagaaatagg cgatcccctt cgccagcttg cagacggctg
    18121 acggcctcct ttgggagaac gacgcctaga gaattgccaa ttttgcggac tttgagttca
    18181 agcatggact cttctccttg ttataacaat tgtagcaact cttagaaggg cgcgcaagtg
    18241 accgaacggc agagttccgt gatcgctact ttgagacggc gtcttggcgg aggcggtaag
    18301 cgagccaggc catggttgcg agcatgccga ggtagccgag ggcgcgcatg atcccgccga
    18361 ttttctggtc ctgggcgagg aggccccact ggctgaaaag aattgtccag tcgctctcct
    18421 cggagccgac gaggggcaat gccgcgctgc gcgcgtcggc catgtaggtg ccgatgtaag
    18481 ggaaattttc gaagaaccag aagttggaga aggcgaagcc ggtggcttcg cgttgccaga
    18541 agaaatagat ggcgcacaag agcgggacga gcagttcgcc gagggttccg ccgagaatca
    18601 tgatggtgtt gccgaaccag ctgaagaaga aatggccgcc ttcgtggatg atcagattga
    18661 cgtaatcgag gatgaggaat ccggagcggt ttgcaagggc gtagagaagg aaaagggcgt
    18721 agaagcatag ccaggcgatt ccggtgaggc gggagacagg cttccagtct ccgaattttt
    18781 cttcgaggaa ggccacttgc tcaagcgtag caagctcgat tcagcagaca aagcagttcg
    18841 gagaaaaatg tttcgatcca cgggactaga aaaaaggtaa tgagttagtt tgaaccgagg
    18901 ccaagagcag tcgcgtgttt gggctgatag agaccaatct cgccgccgct gggcaaccgg
    18961 atgctcgttt ttattcccca agcctcctcg ctaaccggtg agcaactgac gttcttgaca
    19021 tccaacgatt tgatgaggga atgcaagtcg tcgcacatga gatagagaac agcgccaaga
    19081 agatggcgtc ctccatgaag ctgccgattg tctccgtccg agggatgaag tgcggcttcc
    19141 gcaggaggca acgcaaagat cagccaacca ccgccaatat ccactgcggg gaactccaaa
    19201 acgtcgcgaa agaatgcgcg atcagcttcc ggatcgtcgc tgtagaggag gacgtgagcg
    19261 ccgataaaca tggaggcagc ttatcatcct tcgtattgaa cggtttcggc gaagtctgtt
    19321 ctgacgtcaa taaggagcgt cggagcggct acttatcgat ggcggcgagg gcttcgtagg
    19381 cggcgccgag gagcggggcg gattcgttga ggacgatgga gacggggatg ttgctgagga
    19441 ggccttcgaa tttccatttg tccttgaagg cgtgaaagaa agtgccgtct ttcatttttt
    19501 cgagaatttt gacggcgatt ccgccggcga cgtaaacacc gcctagcgcc agaactttca
    19561 gcgcgagatt ccctgcttcg gcgccataga tggcggtcca gagatccaag gtgtcggaac
    19621 agacccggca ggacttgtcc aatccgcggc gagtgatctc cggggcgggg tcggcgtcgg
    19681 gatcatcgaa cgatgggtgg attacctttg gcgcaaggaa ttcatgaaga gtgcggaagc
    19741 cgcggccgga gagaatgagc tcccagctta cttgaggata gcggcggcgc atgaagcgca
    19801 gcagttcgat ctgctgctcg gtatgcggcg cgaaatcgga atgcccgcct tcggaaggca
    19861 cgacgcggta gcgcccgccg tcccacacga ggatagactg gccaaggccg gtaccagccg
    19921 cgagcaatgc ccgcgtcccg cccggctcgg ggttaccggg attaagtacg caaaagtctt
    19981 ccggcggaag atgctcgata ctgtggccgg tggcgccaag gtcattcaag agaacgacgt
    20041 gcgggacatt cagctccttg atgagagtct tggtatcgac gatccacggg agattcgtcg
    20101 ctctgacggt gccattgata accggccctg cgatgccgaa gccagcggca acgatgcttt
    20161 ccttccctag atgttcggcc gcctggcgcg agaattcctt gacgatgcgc tcgaattgcg
    20221 cgaactcttt gctagcgaag cgttgcttga agacgggcgt gagggtgccg cctttttccg
    20281 agaagagcgc caaattacac ttcgtcccac caacgtcccc tgcaaggatc acctgttccc
    20341 cctaccccag ccagtaactt tattttacgt cagatgctgc gattacgggt tgtaccactg
    20401 tctgccgtca cgttcgagca gaacatcgga ttcccttggc ccccaactcc ctgcggaata
    20461 ttgcggcacg gtggcgctct tggccgcact ccagacgtcg aggatgggat ccaccaacga
    20521 ccaagccgct tcgacgctgt cgccgcgatc gaagagcgtg gcatcgccgc gcacacagtc
    20581 gttcaagagc gtggcgtagg cgctgcgcgt cccctcccca aaagcttcgc gatagcagaa
    20641 gttcatggtg acgtttccaa tgctcatttc ggttcccggg cgtttcgcgc cgaaggaaat
    20701 cgaaatgcct tcgtccggct ggatattcaa gatgagacgg ttgggcataa tctctttttc
    20761 gcggaaaact atgtgcgggg cgcggcggaa ctggatcatg atctccgtgg tgcgcttggc
    20821 gagacgcttg ccggttcgaa gataaaacgg aacgccggcc cagcgccagt tgtcgatcag
    20881 caatttgccg gcgacaaagg
//
LOCUS       DASYVO010000234         7279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_133528, whole genome shotgun sequence.
ACCESSION   DASYVO010000234 DASYVO010000000
VERSION     DASYVO010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7279
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133528"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            777..1556
                     /locus_tag="VGR03_07850"
     CDS             777..1556
                     /locus_tag="VGR03_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1994228.1"
                     /translation="MKTLFAGENTIVAFFEWFGELGMFCGRLARSFFVPPYEGREFGR
                     QFDELGAKSLPLAAIAGAATGVVLSLSTRDSLIRFGAKTLLPAIIVISIVKESGPIIT
                     ALVVSGRAAAGIGAELGAMKVTEQIDAIEVSAVDPYKFLVATRVLACILMLPLLTLAA
                     DFCGIVMGWVTNTLAEPISLRLFLENGFRNVTFNDFLPPTLKTMVFGLIIGLVACFQG
                     MRTEGGTGGVEHAATTSVVLSSLLIILADVLLVRLILVFFP"
     gene            1564..2328
                     /locus_tag="VGR03_07855"
     CDS             1564..2328
                     /locus_tag="VGR03_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1994229.1"
                     /translation="MKEEHKTDEKTEPPVQVKALHKSFGKQTVLDGINLEVRQGETLS
                     VLGQSGSGKSVLLKLLVGLQAPDSGSIRVHGKEIAGLPMDQLNEIRKKIGFLFQHSAL
                     YDSLTVEENVAFPLRRHDKMSDAKRKKRARELLTSVGMEQDSQKMPGQLSGGMQKRVG
                     LARALALEPDLLLFDEPTAGLDPITAAEIGELILKLQKERKLASIVVTHDIHIAQTVS
                     DRLALLRDGQILFEGTFEDLRKSRDKFVVQFLNDGS"
     gene            2338..3255
                     /locus_tag="VGR03_07860"
     CDS             2338..3255
                     /locus_tag="VGR03_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV1994230.1"
                     /translation="MSRAFRVGFFVVLALLFLSAGVFLVGNKEFLFSSTYRLKTDFPN
                     AAGLNNGAEVRVGGVHEGTVNRIDLPVQPGGKVTVVMKLHSSTRNIIRKDSIAAIKTE
                     GLLGNKFVEISFGSEKAQAVMNDDVIASESPLDMTDQAKSLAANAQAGVKAFRDDMEA
                     LQQNFLLRGFFNKRGYNDSSELTKHAISKVPQGPPIKEFNYDATQIFDKPDNAQLKDK
                     KALDEAGRFLAENKFDLAVVVSSAVTGDTEKDLVLTKARAKVIRDYLAQNFKLDDTRI
                     RTIGLGKSKKEGDTVKVQILVYASKPAAP"
     gene            complement(3398..3568)
                     /locus_tag="VGR03_07865"
     CDS             complement(3398..3568)
                     /locus_tag="VGR03_07865"
                     /inference="COORDINATES: protein motif:HMM:NF020542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV1994231.1"
                     /translation="MKHKQEFTVVIESDEAGYYVARVPALRGCHTQAKSLDTLMKRVR
                     EVVQLCLVHVKS"
     gene            3635..5008
                     /gene="tssK"
                     /locus_tag="VGR03_07870"
     CDS             3635..5008
                     /gene="tssK"
                     /locus_tag="VGR03_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEV1994232.1"
                     /translation="MQFVDAMKQLQPIIWSKGTFLTPQHLQIQDRFLESVMQFRMESL
                     NFRPWGFRELRINQEELAAGNFALSRATGIFSDGLPFEIPDSDAAPPPKPLATYFEAD
                     VDRLDIHLAIPNYLERGVNISAGRGNVDARYLAEVALIRDENTGRAEKPVQVAKKNFR
                     LLVEGEARHGASTLRVASIVRTPAGLFELDSRFVPPLLDIAASDYLVAILRRLVEILT
                     AKSGLLSGLRRQRNQSLADFTAGDIANFWLLYTVNTHLPILRHLFETKRGHPETLYST
                     LLALTGSLCTFSLRTQPRDLPLYDHEDLGHCYTDLDEKLRELLETVVPSNFVSLPLKL
                     QQTSIYATAIADEKYLKDTKMYLAVSSDKSEADTIRDVPKLIKLCSASQIDQLVRQAL
                     PGIQLTHMTAPPSSIPVKLKFQYYSLNQSGVAWDSVRKARNLAAYVPDDFPNPQLELL
                     ILLPQTA"
     gene            complement(5034..5237)
                     /locus_tag="VGR03_07875"
     CDS             complement(5034..5237)
                     /locus_tag="VGR03_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994233.1"
                     /translation="MRADRVEASWEPSKKKWLVRIQLGEEVIRRLCDVPKDGDEAAIR
                     SAAQKLLGDEGYEPDISQLTIRR"
     gene            5427..6824
                     /gene="tssK"
                     /locus_tag="VGR03_07880"
     CDS             5427..6824
                     /gene="tssK"
                     /locus_tag="VGR03_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEV1994234.1"
                     /translation="MRAQVPEGTGQAEAMKNLSKVVWSEGMYLSPHHFQTQARFFEDS
                     IHFVGASLWYSPFGFSGSKLDAEALRNGTVSVVHARGVFPDGLPFQTPECDPAPEPRG
                     VADIFPPTREFLTVLLAIPPARQSGANCTLAEPNGQPSTRFSSESKSLHDDNTGRDEK
                     PVHVGRKNIRLLFDTEDAGDMVTLPLARVMRDSSGQYVYDPRFIAPCLQINASERLMQ
                     IARRLIEILEEKSAQLARGRRGEGKFRAGFSAQEVAGFWFQHSINAGLSPLRHLYFSK
                     RGHPEELFAEMARLGGALCTFGLESNPAGLPLYDHLHLDDCFEQLDQHIRKHLEIIVP
                     SNCIEITLKLTKKYFYEGEVTDTRCLGRSRWVFALQSNIGEAEMISRTPKLVKISSAV
                     SLPEVVKRALPGLRLTHLPMPPNAISPKVESQYFGVSKTGTLWEQIMETRKVGVYVPG
                     ELPDPEIELLVVLDN"
     gene            6838..>7279
                     /locus_tag="VGR03_07885"
     CDS             6838..>7279
                     /locus_tag="VGR03_07885"
                     /inference="COORDINATES: protein motif:HMM:NF021377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HEV1994235.1"
                     /translation="MEEMSSSATTIGQRAYAPVSSSPRRHENLALVFQEILTAVARLR
                     SNRQAVTDSEAFRGHMRNALKSAESEGVRRGYQPEDVRQALFAVVGFLDESALNSNNP
                     AFSDWARRPLQEEMFGGHFAGEVFFDNLEKLLGGRESADMADLLE"
BASE COUNT         1473 a         2180 c         2126 g         1500 t
ORIGIN      
        1 tggggttagg aggcggggtg cgggtaggtc tagagtgaaa gcgatatctt gaataatcct
       61 cttcgctcgg ctccttccct ccgagccccg gtttctgggc tccggtgtgc cccaccgggg
      121 tcccgagacc ggaccttgat caactaacac ctgccaagtc tgctcgcccg gaaggagccc
      181 acgccgtcat ctaatcagcc ctcagtgtat gactcccaac aggtaggcaa tgagaagaat
      241 caataaaatg gtacctagcc ctacgcccgc acctccaccg taaccccagc ggttatgacc
      301 gtagtaccct ccaccgccgc ccaagagcaa caccagaatg ataatcagta gcagcattgt
      361 ggatcctcct ctcttctgac tcttaactcg cggccggcga catggcgccg agtagggtga
      421 gtgccagcgc aaccagaact gtctttctga tcttcatagt catttccttc tgcgaactat
      481 aagaatttgg tatctattct tacatgggct ggtctgacgc tagaagcttc gaacagaagt
      541 gtctgttcaa ttttggacac cttaaatgtt attgttgcac aatcgtagcc gcggacgatg
      601 gctactttga aggacgagaa gattgctcgg gtggaagtcg cactccccga gcagagcgcc
      661 cacaacggcc agttctagtc cgtctgctac cacctgctgc tgttcaacag actattgcgg
      721 gcgagccgcg cagggtgtaa acctgaactg cagaaatctt taaatcggag cttccggtga
      781 aaactctgtt cgcgggcgag aacactattg tggcgttctt tgaatggttc ggcgagttgg
      841 gaatgttctg cggccggctg gcccggtcat ttttcgtacc cccttacgaa ggccgagagt
      901 tcggccgcca attcgacgaa ttgggagcga aatcgctgcc gctggcggcg attgctgggg
      961 cggccaccgg agtggtcctc tcgctctcga cgcgggacag cctcatccgc ttcggtgcta
     1021 agactcttct tccagccatc atcgtcattt ccattgtcaa agagagtggc ccgatcatca
     1081 cggctttggt cgtgagtggc cgtgcagcag cgggcatcgg cgctgaatta ggcgcgatga
     1141 aggtgacgga acagattgac gccatcgagg tctcggccgt cgacccttac aagtttcttg
     1201 tggccacccg agtcctggca tgcatcctga tgcttccgct gcttaccctg gctgcagatt
     1261 tctgcggcat cgtcatggga tgggtgacca atacgctggc ggaaccgatc tcactccgtc
     1321 tgtttctgga aaacggcttc aggaatgtga ccttcaatga ctttctgccc ccgaccctta
     1381 agaccatggt gtttggtctg atcattgggc tggtggcgtg ttttcaggga atgcggacgg
     1441 agggaggaac cggaggcgtg gagcatgccg cgacgacgtc ggtggtcttg tcgtcgctct
     1501 tgattatcct tgccgacgtt ctactggttc gcttgatcct tgtgtttttc ccgtgaggga
     1561 ccgatgaaag aggagcacaa aacagacgaa aaaaccgagc cgccagttca ggtgaaagcc
     1621 ctgcacaagt ctttcggcaa acagaccgtg ctggacggaa tcaatctcga ggtcaggcaa
     1681 ggagagaccc tgtcggtcct tggacagagc ggcagcggca agagcgtttt actgaaattg
     1741 ctagtcggcc tgcaagcgcc cgactcgggt tcgatccgcg tccacggaaa agagatcgct
     1801 ggtcttccca tggaccagct aaatgaaatc cgtaagaaga ttggattctt atttcagcac
     1861 tccgcgctgt atgattcgct tacggtcgaa gaaaacgtcg cctttccgct gagaaggcac
     1921 gacaagatgt ccgacgctaa acggaagaaa cgcgcgcgag aactactcac gagcgttggt
     1981 atggagcagg attcgcagaa gatgcccgga cagctctccg gaggaatgca gaaacgggtt
     2041 ggcttggcgc gcgcgctggc cctggaaccc gacctactgc tcttcgatga accgactgcc
     2101 ggactcgatc cgattaccgc cgcagaaatc ggggaactga ttctaaaact gcaaaaagaa
     2161 cgcaagttgg catcgatcgt tgtcacgcat gacattcaca tcgcccagac tgtctcggac
     2221 cgcttggcct tactgcgtga tgggcaaatc ctgttcgagg gaacttttga agatctgcgg
     2281 aagagccgcg ataaatttgt tgttcaattc ctgaatgacg gctcttaggt ggccaccatg
     2341 tccagagctt ttcgtgtggg attttttgtc gttttggcgc tcttgttttt gtctgccggg
     2401 gtcttcctgg ttggcaacaa agaattcctg tttagttcaa cctaccgtct gaagacagat
     2461 ttcccgaatg cggccggtct gaataatgga gcggaggtcc gggttggcgg agtgcacgaa
     2521 ggaacggtaa atcgcatcga tctgcccgtt cagcccggcg ggaaggtaac cgtcgtgatg
     2581 aagctgcaca gttcgactcg caacatcatc aggaaagatt ccatcgccgc gattaaaacg
     2641 gaaggcctat tgggcaacaa gtttgttgaa atctccttcg gatccgagaa ggcacaggcc
     2701 gtcatgaatg atgatgtcat cgccagcgag tccccgctgg acatgaccga tcaggccaaa
     2761 tccctggctg ccaacgcgca ggccggcgtc aaggcattcc gcgacgacat ggaagcactt
     2821 cagcaaaatt tcctcttgcg cggcttcttc aataaacgcg ggtataacga ctcgagtgaa
     2881 ttaacgaaac acgcgatttc caaggtgccc caagggcctc ccatcaagga attcaattat
     2941 gatgcaacgc aaatttttga taagcccgat aacgcgcagc tcaaggacaa gaaggccctc
     3001 gacgaggcgg ggaggttcct tgcagagaac aagttcgatc tagctgtggt ggtctcctcg
     3061 gcggtgaccg gcgataccga aaaagatttg gtgttgacca aagctcgcgc caaagtgatt
     3121 cgcgattatc tcgctcagaa ctttaagcta gacgacaccc ggatcagaac catcggactc
     3181 ggaaaatcca aaaaggaagg cgacacggtg aaagttcaga tcctggtcta cgcgtcgaag
     3241 ccggctgcgc cgtagcgcca gaatcaagtc gcagccgggc gggtggccca ggcttgaagt
     3301 ttctgtcttt cgcttgacgg cggcccacca tcggatgatt ctttttgctt gtccccagtg
     3361 aagtgaagag acgcaggctg gggcgaggct cctcggttta cgacttcacg tggaccaggc
     3421 agagttgcac gacttcgcgc acccttttca tcaaggtatc gagactcttt gcctgcgtat
     3481 ggcaaccgcg cagcgcgggc acgcgcgcga cgtagtagcc cgcttcgtcg ctttcgatga
     3541 ccacggtgaa ttcctgtttg tgcttcacgg tcagcctccc caatgacgta gtttaacact
     3601 ccaggatttc tgctttgaca tcagccccaa actgatgcaa tttgttgacg ccatgaagca
     3661 gcttcaaccc atcatctggt ccaagggcac tttcctgacg ccgcagcacc tgcagattca
     3721 ggatcgcttc ctcgaaagcg tgatgcagtt tcgcatggag agcctcaact tccgcccgtg
     3781 gggcttccgc gagctgcgca tcaaccagga agagctggcc gcgggcaact tcgcgctgtc
     3841 gcgcgccacg ggaatcttct ccgacggcct gccgttcgag attcccgact ccgacgccgc
     3901 tcccccgccc aagcctctgg ccacttattt tgaggcagac gtcgaccgcc tggacattca
     3961 cctggccatc cccaactacc tcgagcgcgg cgtgaacatc tccgccgggc gcggcaacgt
     4021 cgacgcgcgc tacctggccg aggtcgcgct gatccgcgat gaaaacaccg gccgcgccga
     4081 aaagcccgtg caggtggcca agaaaaactt ccggctgctg gtcgagggcg aagcgcggca
     4141 cggcgcctcc accctgcgcg tggcctccat cgtgcgcact cccgccggac tgttcgagct
     4201 cgattcgcga tttgttcccc cgctgctcga catcgccgcc agcgactacc ttgtggccat
     4261 cctgcgccgc ctggtggaaa ttctcaccgc caagagcggc ctgctcagcg gcctgcgccg
     4321 ccagcgcaac cagagcctgg ccgacttcac cgcaggcgac atcgcgaatt tctggctgct
     4381 ctacacggtg aacacgcacc tgcccatact gcgtcatctg ttcgagacga agcgcgggca
     4441 tcccgaaacg ctgtactcga cgctgctcgc gcttaccggg tcgctgtgca cgttttctct
     4501 gcgcacgcag ccgcgcgatc tgccgctcta cgaccacgaa gacctcggcc actgctacac
     4561 cgacctcgac gaaaagctgc gcgagctgct ggagaccgtg gtgccgtcga acttcgtttc
     4621 gctgccgctc aagctgcagc agacgtcgat ctacgccacg gcgatcgcgg acgagaaata
     4681 tttgaaagac acgaagatgt atctcgcggt gagctcggac aagagcgaag ccgacacgat
     4741 cagggacgtg cccaagttga tcaagctgtg ttccgcttcg cagatcgatc agctcgtacg
     4801 ccaagcgctg ccgggtatcc agttgacgca catgaccgcg ccgcccagct cgatcccggt
     4861 gaagctgaag tttcagtatt acagcctgaa ccagtcgggc gtggcgtggg attcggtgcg
     4921 caaggcgcgc aacctcgcgg cgtatgtgcc cgacgatttc cccaacccgc agctcgagct
     4981 gctgattctg ctcccgcaga cggcttagga ttcggcgcgg ggcgcttcgc cgcctagcgc
     5041 cggatggtca attgcgaaat gtccggttcg tagccttcgt cccctaaaag tttctgcgcg
     5101 gcggagcgga tggccgcttc gtctccgtcc ttcggcacat cgcaaagccg gcgaatcact
     5161 tcttcaccga gctgaatccg caccagccac ttcttcttgc tcggttccca tgaggcttcc
     5221 acacgatctg cgcgcatgtc tccctccgtc cggaattccc ctgagactac cactcgcgag
     5281 gcatttggtc atctgcccgg tcgcggtgtc caccttggat cgggcgcgcg gtcgcgaccc
     5341 cttcgcgggc ccggaataat agatcacgaa gacgccttta tccctgcgac ctgtgcaagt
     5401 ataatgcccg gcgagcgcgg cttggtgtgc gcgcgcaggt tccggagggc acgggtcagg
     5461 ccgaggcgat gaagaacctc tccaaagtcg tttggtcgga agggatgtat ctctcgccgc
     5521 atcatttcca gacacaggcg cgcttcttcg aagactccat ccactttgtc ggcgccagcc
     5581 tgtggtattc gccgtttggt ttttccggct ccaaacttga cgccgaagcc ctgcgcaatg
     5641 ggacggtgtc ggtggtccat gcccgcggcg tatttcccga cgggcttccc ttccagactc
     5701 ccgaatgcga tccggccccc gagcctcgcg gcgtggccga catatttcca cccacgcgcg
     5761 aatttctgac cgtgctgctg gccataccgc cggcgcggca gagcggcgca aactgcacgc
     5821 tggccgaacc gaatggccag ccaagcacgc gcttctcgtc ggaatcgaaa tctctgcatg
     5881 acgacaacac cgggcgtgat gaaaagcccg tgcacgtggg gcggaaaaac atccgcctgc
     5941 tgttcgacac ggaggatgcc ggggacatgg tgacgctgcc gctggcccgc gtgatgcgcg
     6001 attcctccgg gcagtacgtt tacgacccgc gctttattgc tccgtgcctg cagatcaacg
     6061 ccagcgagcg gctgatgcag atcgcgcggc ggttgataga aattctggag gaaaaaagcg
     6121 cgcagctggc gcgcgggcgg cgcggcgaag gaaaattccg cgcgggattt tcggcgcagg
     6181 aagtggccgg cttctggttc cagcactcca tcaacgcggg actttcgccg ctgcggcatc
     6241 tgtacttctc gaagcgcggc cacccggaag agctgttcgc ggagatggcg cggctgggcg
     6301 gggcgctgtg cacgttcgga ctggaatcga acccggcggg cttgccgctg tacgatcatc
     6361 tgcacctcga cgactgcttc gagcagctcg accagcacat ccgcaagcac ctggaaatca
     6421 tcgtgccctc gaactgcatc gagatcacgc tgaagctgac caagaaatat ttctacgagg
     6481 gcgaggtgac cgacacgcga tgcctgggcc gctcgcgctg ggtgttcgcg ctgcaatcga
     6541 acatcggcga ggcggagatg atttcacgca cgcccaagct ggtgaagatt tcttcggccg
     6601 tttcgcttcc cgaggtggtc aaacgcgccc tgcccggcct gcgcctcacg catctgccca
     6661 tgccgccgaa cgccatctcc cccaaggtgg agtcgcagta tttcggcgtc agcaagacgg
     6721 gcacgctgtg ggagcagatc atggagacgc gcaaggtggg cgtgtacgtc cccggcgagc
     6781 tgcccgaccc ggagattgag cttctggttg tgctcgacaa ttaaggagag cgccgcaatg
     6841 gaagagatga gctcctcggc aacgacgatc ggccaacggg cctacgcgcc ggtctcctcg
     6901 tcgccgcgcc ggcacgagaa tctggcgctg gtgtttcagg aaatcctgac ggcggtggcg
     6961 cggctgcgct ccaaccgcca ggcggtcacc gactcggagg cctttcgcgg gcacatgcgc
     7021 aacgcgctga agagcgcgga atcggaaggg gtgcgccgcg gctaccagcc ggaggacgtg
     7081 cggcaggcgc tgtttgccgt ggtgggattc ctcgatgagt ccgcgctcaa ttccaacaac
     7141 ccggcgtttt ccgactgggc gcgccgcccg ctgcaggagg agatgttcgg cggccatttc
     7201 gccggcgaag ttttcttcga caatctcgag aaattgctgg gcgggcgcga atccgcggat
     7261 atggccgacc tgctcgagg
//
LOCUS       DASYVO010000235         6508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_274545, whole genome shotgun sequence.
ACCESSION   DASYVO010000235 DASYVO010000000
VERSION     DASYVO010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274545"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /gene="ssrA"
                     /locus_tag="VGR03_07890"
     tmRNA           <1..307
                     /gene="ssrA"
                     /locus_tag="VGR03_07890"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(341..3538)
                     /locus_tag="VGR03_07895"
     CDS             complement(341..3538)
                     /locus_tag="VGR03_07895"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994236.1"
                     /translation="MRKSIRCFAAVMMVFLAATIFAGSTPAQEAKFDSDTISGLGARN
                     IGSAAMSGRVAAIAAVKEDGRLTVYVGAASGGVWKSSNGGTTFKPVFDKEAAQSIGAV
                     AIDPQAPKTIWAGTGEAWTRNSVSIGNGIYRSTDGGDNWTNMGLQNSERIAKIIIDPK
                     NSSTVYVCVPGKLWSDSEDRGVYKTTDGGKSWSKILKGANLSTGCSMISMNPQDPKVL
                     FAGMWDFRRKGWTFRSGGENPSAASGSGFFQTTDGGTTWKELDEKSAKGLPAKPWGRV
                     AVTIAPSKPNVVYAMIESTRSALFRSDDGGKTWEERDRSNWMVWRPFYFANLIVDPKN
                     ENKVYKPDLTLIMSEDGGKSFSGIGNGAHGDFHDVWVNPDNTDHVIASDDGGVWYSYD
                     GGNTWWKGNNLPISQFYHVSADNADPYHVFGGLQDNSVWIGDSQYPGGITNGRWENIF
                     GGDGFWVFPDPSDANYVYAESQGGEIGRVNRVTLEGRLIKPQPNYGEGKLRFNWNTPI
                     HMSPNENGTIYIGAQFLFRSRDHGQSWERISPDLTTNDPEKQKQEESGGVTVDNSYAE
                     MHTTIYSISESPRDGQTIWVGTDDGNLQLTRDGAKTWANVAGNVPNLPKASWVSWVEA
                     GLYDAGTAYATFDRHTFGDMDPHVFKTTDYGKTWTPIVAADSGVRGYAHVIKEDTVAP
                     NLLFLGTEFGLWISLDGGKHWAQYKGHQFPMVAVRDIVVHPRESDLVIATHGRGIWIV
                     DDIMPLRKLTPEVMAQDAVFLQAKPSQQRLPANGGWAEGSAVFTGPNSPDAALITYYQ
                     SKRHIFGKMKLEIFDSQGKLVDTLPPNSRRGISRVEWPMRLKAPHVPPAATAAFEAAQ
                     GPRVMPGTYTVKMTRGKETYSTKLVVELDPRAKFTMEDRKLEFDTAMRLHRLLGEMSF
                     DVDRINGVHDALMERGAKLKGDAVFSKRLQELGVKVEAMRKKIVATTKGGAITGEERI
                     REKTTQLYGYILNYEGRPTDYQAARIDSLKKELDDVAGEFDAFVTKELPAVNKTLSQK
                     KLEAIQPIGRKAWDAADADSGAGAPGAAKFFRERD"
     gene            complement(3648..4451)
                     /locus_tag="VGR03_07900"
     CDS             complement(3648..4451)
                     /locus_tag="VGR03_07900"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEV1994237.1"
                     /translation="MKVELRLTPFTKFSRVVRFFLPALAAIAFFSYASSAQTVDEVIA
                     KNIQAHGGVDKLKAVRSIRTTAKFSQGPFRADFRQEKKRVDKVREEFIIQGLAQVQAY
                     DGKTGWQISPFGGRKDPELLSQDDLKSLVVDADLDGPLMEYKEKGHKAELVGHDSVEG
                     TDCFKIKLSMKNRDVRYYYLDADSFLELKLEIQTTIRGALQESELYYGDYEQVNGIYY
                     PFAVEQAQKGSASRSQISVQKIEQNIPLEDAHFTMPVSKPETKAPPVGK"
     gene            4785..5372
                     /locus_tag="VGR03_07905"
     CDS             4785..5372
                     /locus_tag="VGR03_07905"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEV1994238.1"
                     /translation="MRRIIAGVLFFFLFLCAPAVRAQAGPVEGGHELQVWTGGGHGIN
                     GSQSGDGVWNVGFRYGLILTAPHGPGFLRGRLEYAVDVVPVFMVVQKTNTAYGIGINP
                     FAFKWALDTRTSVVPYLELGGGTLFTNTKVPEGTSRINFTTSGAAGLHFLRRKHNISA
                     EVRYMHISNAGLATPNPGINTIQFRLAFGLFSQKE"
     gene            5419..5943
                     /locus_tag="VGR03_07910"
     CDS             5419..5943
                     /locus_tag="VGR03_07910"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEV1994239.1"
                     /translation="MKMIGIYIVVIVLLLGVAVVRAMRTGGKAPSSGTPAPDFTLNSQ
                     DGKPLSLRDLRGKWVVLYFYPKDFTSGCTKEAHNFQRDLRQYEEKNAIILGVSVQDEL
                     THQKFCAKEGLSFKLLADTKYEVSSSYDSLVNLGVAKLSARHTFLIDPAGVVRKTYLN
                     VNAEKHSAEVLADL"
     gene            complement(6073..>6508)
                     /locus_tag="VGR03_07915"
     CDS             complement(6073..>6508)
                     /locus_tag="VGR03_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994240.1"
                     /translation="ISSLLEKNFAGYQQTKELRAWLAELMKRPKEDPIAVAASAVDAK
                     ASALAGEPTPILETPKTTSFMAVNDTLTALMALVDGADFAPSEESFLAYRRICKGSNE
                     ALAAWQELKNKDVAALNTLLGKSNLTMLPDVPNLAADEACGN"
BASE COUNT         1294 a         1947 c         1743 g         1524 t
ORIGIN      
        1 gccggggtcc acctgcccgt aacttggtgg aaactagaaa tgccaacaac aacttggcac
       61 ttgctgctta attaattaag cgcacgcttc cccgggcttc gctcatgggc ccggtcagag
      121 gcgtcacaca gtgagctggc ctaagccttt cggtccgtaa ggctcgggcg agaaccatcg
      181 gactagccgg ctgcgtttct tgctcattct gagcgtggcg ggcgaaacaa acggaattga
      241 gctaagcatg taggcttcgt gtcgtagcgc ttttcggacg ggggttcgac tccccccgcc
      301 tccaccattt cacgcgcaac gtggattgac ttttctaaag ctaatcgcgc tcgcggaaga
      361 acttggcggc tccgggagcg ccagctccgg aatcggcatc ggcagcgtcc caggctttgc
      421 gaccgatggg ctggatggct tccagttttt tctgcgaaag cgtcttattc accgcaggaa
      481 gttccttggt aacgaaggcg tcgaattcgc cggccacgtc atccagttct ttcttcagcg
      541 aatcgatgcg cgcggcttga tagtcggttg gacgtccttc atagttcaaa atgtatccat
      601 acagttgcgt cgttttttca cggatgcgct cctcaccggt gatggcgcct cctttcgtcg
      661 tggccacgat cttctttcgc atggcttcta ctttgactcc gagttcctgc agccgcttgc
      721 tgaagacggc gtcgcctttc agctttgcgc cgcgctccat gagcgcgtcg tgcacgccgt
      781 tgatgcggtc cacatcgaaa ctcatctccc cgagcagacg gtggaggcgc attgccgtgt
      841 cgaattccag tttgcggtcc tccatcgtga atttcgcgcg cggatcgagc tccacgacga
      901 gttttgtgct gtaggtctcc ttgccgcgcg tcattttcac cgtgtaggtg cccggcataa
      961 ctctcgggcc ctgagccgct tcaaatgcag ccgtggccgc gggcggaacg tggggcgcct
     1021 tcaggcgcat gggccattcc acccggctga tgccgcggcg gctgttcgga ggaagcgtgt
     1081 ctaccagctt gccctgcgaa tcgaaaatct ccagtttcat ttttccaaaa atgtgacgct
     1141 tgctctggta gtaggtaatc agagccgcgt ccggggaatt gggtccggtg aacaccgcgc
     1201 tgccttccgc ccagccgccg tttgcgggca gccgctgttg cgaaggctta gcttggagga
     1261 acactgcgtc ttgcgccatg acttcgggag tcagcttacg caggggcatg atgtcgtcca
     1321 cgatccaaat gccacggcca tgcgtggcga tcaccaaatc cgattcacgc ggatgcacaa
     1381 cgatgtcccg gacagccacc atcgggaatt ggtgaccttt gtattgcgcc cagtgtttac
     1441 cgccatccag tgaaatccac agaccgaatt cagtgcccag gaatagcaga ttgggcgcca
     1501 ccgtgtcttc cttgatgaca tgcgcgtagc cgcgcacacc gctgtccgcg gctacgatgg
     1561 gcgtccaggt cttcccatag tccgtggtct taaacacgtg tggatccatg tctccaaatg
     1621 tgtggcgatc gaacgttgca taagccgtgc ccgcgtcgta gaggccggct tccacccacg
     1681 aaacccacga ggctttcggc agattcggaa cattgccggc gacgttggcc caagtcttcg
     1741 cgccatcgcg cgtcagttgg agatttccgt catcggttcc aacccagatc gtttggccat
     1801 cgcgcggcga ctcgctgatg gaataaatcg tcgtgtgcat ctccgcgtaa gaattgtcaa
     1861 ccgtgacgcc gccggattct tcctgtttct gtttctccgg atcgttggtg gtgagatccg
     1921 gcgaaatgcg ctcccacgat tgaccgtggt cgcgggagcg gaagaggaat tgcgcaccga
     1981 tgtaaatcgt gccattttca ttggggctca tgtggatcgg cgtgttccag ttgaagcgca
     2041 gcttcccttc tccataattg ggctgcggct tgatcaaccg gccctccaga gtgactcgat
     2101 tcacacggcc gatctcgccg ccttgtgatt ccgcgtaaac gtagttcgca tcggatggat
     2161 ctggaaatac ccagaaccca tcccctccaa aaatattttc ccagcggccg ttggtaatcc
     2221 cgcccggata ctgggagtcg ccgatccaca cgctgttgtc ctgcaacccg ccgaaaacgt
     2281 gataggggtc ggcgttgtcc gcgctcacgt gatagaactg cgagatcgga aggttatttc
     2341 ccttccacca ggtattgccg ccatcgtagg aataccaaac gcctccgtca tcgctcgcga
     2401 taacatgatc ggtattgtcg ggattcaccc agacatcgtg gaaatcgccg tgtgcgccgt
     2461 tcccgattcc gctgaagctc ttgccgccat cctcgctcat gatcaaggtg aggtccggct
     2521 tgtaaacttt gttctcgttt ttcggatcga cgatcaggtt ggcgaagtaa aagggccgcc
     2581 agaccatcca attgctgcgg tcccgctctt cccatgtctt gccgccatca tcggagcgga
     2641 aaagagcgct gcgcgtcgat tcgatcatcg catacacaac atttggtttt gatggcgcga
     2701 ttgtgaccgc tacgcgcccc cagggtttcg cggggagccc tttggcgctc ttctcatcca
     2761 gttctttcca ggtcgttcca ccatccgtgg tctggaaaaa accgctgccg ctggccgcgc
     2821 tcggattctc tccgcccgaa cggaaggtcc agcccttccg gcgaaaatcc cacatgcccg
     2881 cgaaaagaac cttcgggtcc tgcggattca tggaaatcat cgagcagccg gtcgagagat
     2941 tcgcgccctt cagaatcttg ctccagctct tgccgccgtc ggtagttttg taaacgccgc
     3001 ggtcctcgct gtcgctccac agttttccgg gcacgcagac gtaaaccgtg ctgctgttct
     3061 tcggatcgat gataattttc gcgatgcgct cggaattctg taagcccatg ttggtccagt
     3121 tatcgccgcc gtctgtcgac ctgtatatgc cattgccgat cgacacgctg ttgcgcgtcc
     3181 aggcttcgcc cgtgccggcc cagattgttt tcggcgcttg cggatcaatg gccacagcgc
     3241 cgatggattg cgcggcctcc ttgtcgaaaa cgggcttgaa cgtcgtgccg ccattcgaag
     3301 atttccagac gcccccgctg gctgctccaa cgtagacggt cagccgtccg tcctctttta
     3361 ccgccgcgat cgccgccacg cgcccgctca tggcggcgga gccgatgttc cgcgcaccga
     3421 gaccggagat cgtgtccgag tcgaatttgg cttcttgcgc gggcgtggac ccggcaaaaa
     3481 tcgtcgcagc gagaaacacc atcatcacgg cagcgaagca gcgaattgat ttgcgcatat
     3541 cgtcagattc cttagcgaaa tagttgaagc aactgctctt gcgtgtgagt ccatccacga
     3601 atattcggaa gtaggacaaa cattcttgcc tgtcctctgt tcaagaatca ctttccgacc
     3661 ggcggcgcct tcgtctccgg tttggacacc ggcatcgtga aatgtgcgtc ttccagcggt
     3721 atgttttgtt cgatcttttg gacggaaatc tgcgagcgtg aagcgctgcc tttttgcgcc
     3781 tgctcgacgg cgaagggata ataaatgccg ttcacctgtt cgtagtcgcc gtaatacaac
     3841 tctgactcct gcagcgcgcc gcgaatggtc gtctgaattt ccaatttcaa ttcgaggaac
     3901 gagtccgcat cgagatagta gtagcgcacg tctcggttct tcatgctcag cttgatcttg
     3961 aagcaatccg ttccttcgac cgagtcgtgc cccacgagtt cggctttatg ccccttctcc
     4021 ttgtactcca tcagcggccc atcgagatcg gcatccacca ccaggctttt aaggtcgtcc
     4081 tgagagagca gctctggatc tttgcgcccg ccgaagggag aaatttgcca tcccgtcttg
     4141 ccgtcgtaag cctgcacctg tgccagccct tggatgatga attcttctcg aaccttgtcc
     4201 acgcgtttct tttcctgccg aaaatcggcg cgaaacgggc cctggctgaa ttttgcggtg
     4261 gtgcggatgc tgcgcacagc tttcaacttg tcaactccgc catgcgcctg aatattcttg
     4321 gcaatgactt catcaacggt ttgtgcgctg gaggcgtacg aaaagaatgc gatggccgcc
     4381 aatgcgggaa ggaaaaaacg gacaaccctg gagaacttcg taaacggagt cagccgcaac
     4441 tctactttca tgaactcccc ccaactcaac ggtatgtgac aataacccaa acagaaggga
     4501 ccgaaacggc gaacctttta ttactccgac gaaatcagca gcacttagtt agcagacttt
     4561 cgcccttctg ccaagaagtt tcgtcgcggg atatccgagc gccgagggta gcgctcgcac
     4621 cggcctccct tttgccaaaa gcgaagacca agtgtggatc ccggcgcctt tccaatttct
     4681 cagcagccta ttagaagagg gtcgcctttc gcgcggattt tctatatctt ctctgcaacc
     4741 ggaactcgct gtaccggatc gaaaaacatg agggagcttt ttcgttgaga agaataatcg
     4801 cgggcgtcct cttttttttc ctttttcttt gcgcgccagc agttcgcgcg caagccggcc
     4861 cggtcgaggg cggccatgaa ttgcaggtgt ggaccggcgg cggccatggc atcaatggca
     4921 gccagtccgg cgacggcgtc tggaacgtgg gcttccgtta cggcctgatc ctcactgcgc
     4981 cgcacggacc cgggttcttg cgcggccgct tggaatatgc ggtcgacgtc gtcccggtct
     5041 tcatggttgt tcagaaaacg aacaccgcct atggcattgg cataaaccct ttcgccttca
     5101 aatgggcgct ggacacccgc accagcgtcg taccgtatct cgaacttggc ggcggcactc
     5161 tctttaccaa taccaaggtg cccgaaggaa cttcgcgcat caatttcacc accagcggcg
     5221 ctgcaggcct gcacttcttg cgccgcaagc acaacatcag cgcggaagtt cgctacatgc
     5281 atatttcaaa tgccgggctt gcaacgccaa atcccggcat caataccatt cagtttcgtt
     5341 tagcctttgg tctgttcagc caaaaagaat agcagcattg caacggctcc gattcagaac
     5401 caggattgag tgtgcgagat gaaaatgatt ggaatctata ttgttgtaat cgttttgctt
     5461 cttggagtcg ctgtcgtccg cgcaatgcgc accgggggga aagcgccgtc ttccgggact
     5521 ccggcgccgg actttacgct gaattcgcag gatggcaaac cgctcagcct ccgcgatctc
     5581 cgcggcaaat gggtcgtcct ctatttctat cctaaggatt tcaccagcgg atgcaccaag
     5641 gaagcgcata attttcagcg cgacctccgg cagtatgaag agaaaaacgc gatcatcctc
     5701 ggtgtgagcg ttcaggatga acttacccac cagaagtttt gcgccaagga aggcctaagc
     5761 ttcaaactgc tcgcagatac gaagtacgag gtctcttcat cctacgactc gctcgtgaat
     5821 ctaggcgtcg ccaaactttc cgcccgccac acttttctca tcgatcccgc aggcgtcgtc
     5881 cggaaaacct atctcaacgt gaacgccgaa aagcacagcg ccgaagtcct cgcggatctc
     5941 tagcaactcc agcagcctct agccgcaatg taggggcgca gcatgctgcg ccctgcccgg
     6001 caacaccgtt cccaccagag cggtcttgct gtgcttctgg actgcaccaa agacccttgt
     6061 gagaaaagcg gtttagtttc cgcaagcctc atcggcagct aaattgggaa cgtcaggcaa
     6121 catcgtaaga ttgctcttgc cgagcaaggt attcagcgcg gccacatcct tgttcttcag
     6181 ttcctgccag gccgcaagtg cttcgttcga acccttgcag atcctccgat acgccaggaa
     6241 tgattcttcc gacggcgcaa agtctgctcc atcgaccagc gccatcagcg ccgtcaaagt
     6301 atcgttcact gccatgaagc tggtagtctt gggagtctcc aaaatcggag tgggctcacc
     6361 cgcaagcgct gaggctttcg catccactgc gctggccgca actgcaatgg gatcttcctt
     6421 gggacgcttc atcagctcgg ccagccacgc gcgcaactcc ttcgtctgtt ggtaccccgc
     6481 aaagtttttc tcgagcaacg aggagatt
//
LOCUS       DASYVO010000236        14214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_230366, whole genome shotgun sequence.
ACCESSION   DASYVO010000236 DASYVO010000000
VERSION     DASYVO010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 14214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14214
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230366"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            946..4278
                     /locus_tag="VGR03_07920"
     CDS             946..4278
                     /locus_tag="VGR03_07920"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEV1994241.1"
                     /translation="MAAYGLACAFLGDTKMNLNWLVVVCLDSLAENPRSTSRSSWVRL
                     GLKLLALCMTLSCLPSARAQVSASIKGTVTDSSGAPVPSANVKTKNLDTGTIRSGITD
                     DAGRYLMLSLSVGEYEVRVTKAGFQDVIRSGIHLVVGEEANLDLRLQVGGVKSEITVT
                     GDAPMVSTTTTDISGLVGEQAIKELPLNGRSYDLLLPLNPGIVNFTSQKTGGTGISNS
                     TTANNFAVSGNRPQQNLFLLNGVEYTGAAENNMQPGGTSGMLLGVDAVREFNVQRDSY
                     SAEFGKRPGGQVIILTRSGSNQLHGAVFEFLRNNALDAPNFFDQGLAPPFQRNQFGAS
                     LGGPVRKDNTFLFCNYEGFRQNLHQTSVAFVPSLASRAAAAPSVQPLLNLWPTPAPGA
                     QDFSGISQVFSSPPQTIREDFGTVRLDHTFSPKDSLAAIYTIDDGGDVTATPLNPYSS
                     DMVTLREQVLSVEETHVFSPSFLNTARFGFSRAGYFFTGEPTPGTPAANVPGFLIGHP
                     VGAVVVGGSAASNPQAQLGLAGSNNGSNLRIARNLYTLEDHVTLTRGRHQLEFGAWFQ
                     RFQSNETIALSQFGQATFTSLQTFLLGTISSFLFDPSPTEMNWRSLLGAGYAQDTLRI
                     NRRLTVTLGFRAEFTTGWNEAHGRASNYTFTNGVISTQPRIGDSLFTVNNAKFLPQLR
                     IGVAWSPFSAKTVIRAGFGMYNDLQDALGYRADQNAPFNPTYTTPLTVAQLPIDPAGP
                     FPATAKFIPGGVQPDMQTPTLISYSLRIQQELSPNTSLTIGYVGSHGYHELIGIDANE
                     PFPVICPASPCPATYPKTFPAGIASTPVPAGTYFVPTTTRANPAIANTWTWFSEGTSS
                     YNSLQVDVNRRFSGGLTLRGAYTFSKVLDDGDSLNATTSGGGPALASNPFNLKADWGL
                     GTFDIRHVAVVSGTYALPVGHGKRFLGGLEGLGNAAVSGWTLNSIVTLQGGFPFSPQL
                     SYNPSNNGDTRNPVRPFANPAFMGPLILGNPAQWFNPAAFLAPANTPANGGFYGNVGR
                     DSLIGPGLATWDFSVLKDTRIRERLNLQFRAEIFNLLNRANFNQPNAVVFTPSGVSPT
                     AGVITSTSTTSRQIQFALKLLW"
     gene            complement(4262..4528)
                     /locus_tag="VGR03_07925"
     CDS             complement(4262..4528)
                     /locus_tag="VGR03_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994242.1"
                     /translation="MTIRVDLKPETEARLVAEAWAQGLPLEELAERLLKEALTSHSPS
                     HGQMSVEEFHRMLEAMASGSEKLPDLPTESFSRQSFYEAPTKAI"
     gene            complement(4593..4895)
                     /locus_tag="VGR03_07930"
     CDS             complement(4593..4895)
                     /locus_tag="VGR03_07930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEV1994243.1"
                     /translation="MAKKLKPVHPGEILREEFMIPLGLSMNKMAMALRVPVTRIADIV
                     NERRGITADTALRFARYFNNSPAFWMNLQTRYDLEVAEDEIAPKVARDVQPLAEAR"
     gene            complement(4905..5183)
                     /locus_tag="VGR03_07935"
     CDS             complement(4905..5183)
                     /locus_tag="VGR03_07935"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1994244.1"
                     /translation="MIRSFRCADTERLFHREAVRRFKAIERQALRKLDMLDAAPDLRT
                     LSTLPGNRLEPLKDDRKGLHSIRINDQWRICFAWREGHAYEVEIVDYH"
     gene            complement(5328..5870)
                     /locus_tag="VGR03_07940"
     CDS             complement(5328..5870)
                     /locus_tag="VGR03_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV1994245.1"
                     /translation="MRKVVLGLGISLDGYIARPDGAVDFLFMPKDYSMGPFFATIDTA
                     IMGRKTYDVALKMGGGGFSDSKMKNYVFSHSQPPGERGGITFVNESPKSFLEQLRKRP
                     GKNVWLMGGGELAREFLKEDLVDELYIGIVPVLIGEGIPLFPSGFPQREFSLLENKSF
                     SKGLIALKYARTRKKTKRKL"
     gene            6043..7680
                     /locus_tag="VGR03_07945"
     CDS             6043..7680
                     /locus_tag="VGR03_07945"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HEV1994246.1"
                     /translation="MQKLVLAAVGTVLLSCGAYGQNAPSGTPSCEGLAQLELSGAKIL
                     SARTVAAGAFTPPSNMTPWLVGDASFYKMLGAFCRVVVTATPSADSSIKIEVWMPANS
                     QKEGGWNGKLQGQGNGGFAGEIGYHQMGIAVHQGYATVGTDTGHSGGGTDASWALGHP
                     EKVTDFGYRGIHEMTRVAKAAIKAFYGSDPQHSYFGGCSNGGRQALMEAQRFPEDYDG
                     ILAGAPANYWTHLLTKALADAQTTTLDPASYIPASKLPAIARAVDAACDAQDGVADGI
                     VNDPRKCHFDPAGMLCKEGDAEKCLTAAQVTALKKLYDGPHDAKEREIFPGYVPGAEE
                     GPGGWATWITGAAPGKSLLFAFSGGYFSNMVYEKADWNYKTTSVDEAVKAADEKTAQI
                     LNATEANLVAFKARGGKLILYHGWNDPAISALNTINYYNDVTNRMRGQEPEAFVRLYM
                     VPGMQHCGGGTGPDSFGQGGAGAKDAQHNVELALEQWVEKGIAPNAIVATKYEGGDPA
                     KGAKMTRPLCPYPQMAKYKGKGDTNDAGNFVCYAESK"
     gene            7770..8261
                     /locus_tag="VGR03_07950"
     CDS             7770..8261
                     /locus_tag="VGR03_07950"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEV1994247.1"
                     /translation="MSRKSMVKIFVVSLVVLGALGSAVAQMPNPYGPAISLENAKKAA
                     APALAEAARNNWTMAVAIVGPAGTLVYFEKMDNTQIGSAEVAIDKARSAALFKRPTKA
                     FQDAVAAGGDGLRVLGLKGAVPIEGGIPLVLDGRIVGAIGVSGGTSAQDGQCAKAGAD
                     TVK"
     gene            8281..8778
                     /gene="folK"
                     /locus_tag="VGR03_07955"
     CDS             8281..8778
                     /gene="folK"
                     /locus_tag="VGR03_07955"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV1994248.1"
                     /translation="MAEKTVYLSLGSNVGDREENLRAALAALEDAGVRVTQVSSFYET
                     EPVDFLEQAWFLNCAVEGKTDVPALELLRTLRGIETRMGSKKLVAKGPRLIDMDILLY
                     GSETIDTPELQVPHPRMHLRRFVLVPLAEIAAEVRHPSLIKTVKQMLEGTPDHSTVER
                     FDPAK"
     gene            complement(8825..9964)
                     /locus_tag="VGR03_07960"
     CDS             complement(8825..9964)
                     /locus_tag="VGR03_07960"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HEV1994249.1"
                     /translation="MERSIFAALIGGLLGITALGGLAFVGSVPGAPAAPSSRPEGDWR
                     LLDPVTYENISVFPVVSSFSQDTSPFLTLEEGLATGEVTVREQGSETMVRGRDGRPVY
                     VPQSGTGASVNQLVLINRSRRPLLLLAGELVSGGKQDRVIGKDRIIPAGAPPLPLDVF
                     CVEHGRWTGSAQFAAANTIVHPSVRESVAVDQAQTKVWYSVRNGTNAKAPPAAPRAQI
                     SAENLQSAIAGNGRTEAYEKIYESRAVGVSIDGFVDEVQRRFVQATSGLKNERVVGVV
                     VAYGGEVAWSDIFASGDLFDHYSRKLLHSYAVEALTRPTYRQAASRDNANEFLRRLNG
                     RETQETEPGVYRWREIKEGQLAQIELDALQPKAMTLHRLLLHRTS"
     gene            complement(10169..10792)
                     /locus_tag="VGR03_07965"
     CDS             complement(10169..10792)
                     /locus_tag="VGR03_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994250.1"
                     /translation="MSTSPTPAPQQVGILVLDNDAPGGSAVKQILDSEGWRVRIVPDT
                     KMLLAELKTGEWSLVIADVALTGIEGPAFVTLRELASVSLDDGGRIRVLFLIPELAGG
                     QYIGLLEAARLPYVLRPYHLHDFLEKVSDLLVEVKAIEGPIRQVRHEFGALRKKKKQA
                     GRSNSMFASRDSFSYTDEELAEYEKQESEASSSKRAKPRVNLGDPNR"
     gene            complement(10901..12268)
                     /locus_tag="VGR03_07970"
     CDS             complement(10901..12268)
                     /locus_tag="VGR03_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994251.1"
                     /translation="MPEKAKRALVLAFAILVCLPMLSLGQNPPLAKNLPTGRPAPQST
                     HYPILLLAFGNDPNWSLRIGQKGPERLDRPGYPSISLEPTDVSHEAAADSWTYHAKDS
                     ATGAAVAVHLSREACTDAKDMLIPTPPPAVKYPFRASVDHAQLGTLKGCARIAAELFP
                     KINNQPDDEEDAAKKKPPAPTITNFKPPVVVAYLNAAGNVVVSRGAVKKIAAQVGTEL
                     ALSHSGKKLLYVRSDSTTGPDRTIVLYDLDTGKSVDLVHGLVRQPFWSPDDARIAFLK
                     ALDRSWQVWSFPVATPEVAISFSPQPVNSLHGWIDNRTLLASDMLNAYWLSEEVLPQA
                     VALKDIYGATFQSISSDTIRVHPINPDLLLVSADYATAPVGTPVDSMGLAAGFFLFEL
                     RSKRRVTLCPPDQWSRGAEWSRDGLQVFYTRSISGSPAATFRIFWDGTGVQKYTSGSG
                     LVVGQ"
     gene            12873..13859
                     /locus_tag="VGR03_07975"
     CDS             12873..13859
                     /locus_tag="VGR03_07975"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV1994252.1"
                     /translation="MKSILNRCDSNRVPFEWTINPYRGCEFACKYCYARYTHEYMELD
                     GSEFEKKIYVKKDAAPLLAWDVAHKYSYSSEASGGARPDHIAIGTATDPYQPAEREYG
                     VTRACLEELAKREGLSVSIITKSNQIVRDIDVLQRIAERSNLGIDITITTLRAGLTRL
                     LEPRAPRPDLRLAAVKQLREAGLAVGVSASPLIPGITDRDGDLEAVAAAAKEAGAQWF
                     FSGVLFLMPSSARQFLPFVREKFPRLARQYEEWYAKNGYAPEEYRKKASQRVARIREK
                     YGFAARPWVEAKRKAPCSQMSLSWDAGLAAPPGGMFVRSAIGEPEVRAAAGR"
     gene            complement(13910..>14214)
                     /locus_tag="VGR03_07980"
     CDS             complement(13910..>14214)
                     /locus_tag="VGR03_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994253.1"
                     /translation="TYKTLSSGIIYEKPPDAPLPRELYAALIALISEVKKQQAERTGA
                     AALKDMDLFYLWVFLYRMGLLRTNGRPRSRRFIEFLRGQFPHAPELKREESRIIVP"
BASE COUNT         3056 a         3616 c         4702 g         2840 t
ORIGIN      
        1 gacgagtcgc cactgcacgc gcctacgaat acttcggccg cgaattgccg aagcgtcagc
       61 cgcgactgtt ctaattggtc agaaggacca acgctacccc accccccatg ttttttgtaa
      121 gtgcggattc caaagggttt acggaccacg gattgcgtga gtgcggattc taaaggggtt
      181 ataagtcctt tagaatcagc agatccgagg ggggcttgta agtgcggatt ataaaggggt
      241 tgcgggaggg gggtcttgga tgtgcggatt acaaaagact tagaggcgag tggcgcgtgt
      301 tgaatggcga gatttggcat ggggcgagag ggggtacccc cccgtcacga cgaaaagagt
      361 gcggaagtga ttgatgagca aagagttgcg ggatggccgt cgcggaaaag agtgcgcaag
      421 cttttgaaaa gaaaagactt gaatgtggac aaggcagaaa tggagatggc gaagggaggg
      481 aaggccggga ctggtgagcg gcaaacacgc ggaactgtag catcaaagaa tatctggtgt
      541 caagtaatta cttagatagc attcgaatgg agcgggaagg tgttgaaaag gaagaggttg
      601 agagattgat gagcggagtc taaggaatag ggatgcaggg cgggaaagcg gaaaaccttg
      661 agaaaaggag gcggagcagg aagacggtgg aagagtcaaa acccacacgc ttcaaaaaac
      721 gaagcatccg gcacccacat cttgcctcgg gaagacgggc ccgccacgcc cgaaatcaga
      781 tccaggtatt tctcttggtt gttttactcc tcgcaaacga atcttgacgc gacaagtcgt
      841 ataaattcac ttacatctta ttcggattaa gtcggtttga cataacggta cgaaccgtat
      901 tgaattgtgg cctagcagca tcgcggctgg tctgaatggc gtgacatggc agcctacggg
      961 ctcgcctgtg cctttctggg ggataccaag atgaacctga attggttggt ggtcgtttgt
     1021 ctagattcac ttgcggagaa tccacgtagc actagccgat caagctgggt acggcttggt
     1081 ctcaagctgc tggcgctttg catgacgctg tcttgtttgc cgtcggcccg cgctcaagtc
     1141 tccgcgagca ttaaaggcac ggtcaccgac tcatcgggag caccggttcc ttcggcgaac
     1201 gtaaagacaa agaatctcga tactggaaca attcgaagcg gcatcacgga cgatgcaggc
     1261 cgctatctca tgctctcgct gtctgttggt gagtacgaag tgcgggtcac caaggcgggc
     1321 tttcaagatg tcatccggag cggcatccac ctggtcgtgg gcgaggaggc caacctcgat
     1381 cttcgattgc aagtgggcgg ggtgaaatcc gaaatcaccg taacaggcga cgctcccatg
     1441 gtgagcacca ctactacaga tattagcggc cttgtgggag aacaggccat caaggaactt
     1501 cctttgaatg ggcggagcta cgacctactc ttgccactca atccgggtat cgtcaatttc
     1561 acttcacaga agacgggcgg gacggggatt tccaactcaa ccacggcaaa taacttcgcc
     1621 gtctccggca atcgcccgca gcaaaatcta ttcttgctga acggagtgga gtacaccgga
     1681 gcggccgaga acaacatgca gccgggcgga acgagcggga tgttgttggg ggtcgatgcc
     1741 gtgcgtgaat tcaatgtcca gcgcgactcc tacagcgccg aattcggcaa acggcccggc
     1801 ggccaagtaa tcatcttgac caggtcggga agcaaccagt tgcatggagc cgtgttcgag
     1861 ttcttgcgga ataacgctct cgacgcaccg aacttctttg atcagggttt ggccccgccg
     1921 tttcaacgca accagtttgg agcttcgctg ggtggaccgg ttcggaagga caacaccttt
     1981 ctcttctgca actatgaagg atttcgtcag aacctccatc agacgtccgt ggccttcgtc
     2041 ccgagcctgg catcaagggc ggcggcggcc ccgagtgttc agcccctctt gaatctctgg
     2101 cccacacctg ctccgggagc gcaagacttc agtggcatct cccaagtctt tagcagtcca
     2161 ccgcaaacga tacgcgagga ttttggcact gtccggctcg atcatacgtt ttcgccgaaa
     2221 gactcccttg cggcgattta caccattgac gacggcggtg acgtcaccgc gacgccactc
     2281 aatccctaca gttcggacat ggttaccctc cgcgagcagg tcttgagtgt cgaggagacg
     2341 cacgtcttct ccccatcgtt tttgaacaca gcccggttcg ggttttcgcg tgcaggctac
     2401 ttttttacgg gagagccgac accgggtact ccagcggcaa atgttcccgg attcctgatt
     2461 ggacatcccg tcggggcggt agtggttgga ggcagcgcgg catcgaatcc gcaagcccag
     2521 ttaggactgg ctggaagcaa caacggaagc aatctgcgaa tcgctcgaaa tctgtacacg
     2581 ttggaggacc acgtgacgct gacgcggggg cggcaccagc ttgaattcgg cgcatggttc
     2641 cagcgctttc aatcgaacga aacaattgcg ctgagccagt ttggacaggc gacgttcacc
     2701 agcctgcaaa cgtttttgct gggaacaatt tctagtttct tattcgatcc gtcacccacg
     2761 gagatgaatt ggcgctcgct tctgggcgcg gggtatgcgc aagacaccct tcgcatcaat
     2821 cggagactta cagtgacgct gggtttccga gctgagttta cgactggctg gaatgaggcg
     2881 cacggccgag cttcgaacta tacgtttacg aatggagtga tttcgacgca accgcgtatc
     2941 ggagattcgc tgttcaccgt gaataacgca aagttcttgc cacagctacg gattggagtg
     3001 gcgtggagtc cgttcagcgc gaagaccgta attcgcgcgg gattcggaat gtacaacgat
     3061 cttcaggatg ctttgggata ccgcgcggac cagaatgcgc cgttcaatcc gacttacacc
     3121 acgccgttaa cggtggcaca gctcccgatc gaccctgcag ggccatttcc agcgacggcg
     3181 aagttcatcc ccggaggggt ccagccggat atgcagacgc cgacgctgat ttcctattcg
     3241 ttgaggatac agcaggaact ttcaccgaac acctcgctga caatcggcta cgtcggatca
     3301 catgggtatc acgaactgat aggcattgat gcaaacgagc cttttcccgt catctgcccg
     3361 gcgtcaccgt gtccagcaac ttaccccaaa acctttcccg cggggatagc aagtacgcct
     3421 gtgccggctg gaacctactt tgtccccacc accacgaggg ccaatcctgc aatcgcgaat
     3481 acgtggactt ggttttcgga aggaaccagt tcttacaact cgctgcaagt ggatgtgaat
     3541 cgccgcttca gcggaggctt gacgctgcgg ggagcatata cgttctcaaa ggttctcgac
     3601 gacggggact ctctcaatgc gacgacgtcg ggaggcggac cggcgctggc ttcgaatccg
     3661 ttcaacctga aggcggattg gggcttgggg actttcgaca tccggcacgt ggcagtggtg
     3721 agcgggactt atgcgctgcc ggttggccac gggaagcgct ttcttggtgg tttggaggga
     3781 cttgggaacg cggcggtgag cggttggacg ctgaactcca tcgtgacgtt gcagggcggc
     3841 ttcccgtttt cgccgcagct cagttacaac ccttcgaaca acggggacac gcgcaatccg
     3901 gtgcgcccct tcgcgaatcc ggcttttatg gggccgctga ttttgggcaa cccggcgcaa
     3961 tggttcaacc cggctgcgtt tctggcaccg gcgaacacgc ctgcaaacgg cgggttctat
     4021 ggaaacgtag ggagagacag cttgattggg ccgggcctgg ccacctggga tttctccgtg
     4081 ctgaaagaca cgcggattcg tgagcggctg aatcttcagt ttcgggcgga aatatttaac
     4141 cttcttaacc gggcgaattt caaccagccc aacgccgttg tcttcactcc ctcgggcgtt
     4201 tcgccaacgg caggcgtgat taccagcacg tccacaacat cacgtcagat ccaattcgcc
     4261 ctcaaattgc tttggtaggg gcctcataga aactctggcg cgaaaagctt tccgttggta
     4321 aatcgggcag tttttccgat ccgcttgcca tggcttcaag catgcgatgg aattcctcga
     4381 cgctcatttg gccatgagaa ggagaatggc tggtcagggc ttctttgaga agccgttcag
     4441 ccaattcttc taaaggcagc ccctgagccc atgcttcagc cacgaggcgg gcttctgttt
     4501 ctggcttgag gtcaacccgg atggtcatct tagtgcgtct ctgccattta gcttaaacgt
     4561 gttcggaccg aaatactacc cgctgttcca aatcatcggg cctccgccaa cggttgcacg
     4621 tcgcgcgcga ctttgggggc gatctcgtcc tcggcaactt ccaggtcgta gcgcgtctgg
     4681 agattcatcc agaaagcggg gctgttgttg aagtagcggg caaagcgcag cgcggtgtcg
     4741 gcggtgatgc cgcggcgctc gttgacgata tccgcgatgc gcgtcacggg cacgcgcaaa
     4801 gccatagcca tcttgttcat gcttaatcca agagggatca tgaactcctc gcggagaatc
     4861 tctcccggat gcactggctt cagtttcttg gccattttta tctcctaatg gtaatccacg
     4921 atctcgactt cataggcgtg cccctcgcgc cacgcaaagc aaatgcgcca ctggtcattg
     4981 atgcggatgc tgtgcagacc tttccgatcg tctttcagag gctccagccg gtttcccggc
     5041 aatgtcgaga gcgttcggag gtccggcgct gcgtcgagca tgtccagttt tcgaagggct
     5101 tggcgctcaa tggccttgaa ccggcgtacg gcttctcgat ggaaaagacg ctcggtatca
     5161 gcgcatcgga aggaccggat cacctctaga agctatactg aagtccggta aatgtcaaac
     5221 agtacagcct tcgtcaggaa cgaagaatgg tgacgtcccg actgggtcgg gataaattcc
     5281 gtcgccgcaa cggggagaag aaaaagagcc ggactaaagt ccagcggcta caatttgcgc
     5341 ttggtttttt tgcgggtgcg ggcgtacttg agggcgatca ggcctttcga gaaggatttg
     5401 ttttcgagga gggaaaactc gcgttgcggg aagccgctgg ggaagagcgg gatgccttcg
     5461 ccgatcagga ccggaacgat gccgatatag agctcatcca ccagatcttc tttgaggaac
     5521 tcgcgggcga gttcgccgcc gcccatcagc cagacgtttt ttccggggcg cttgcggagc
     5581 tgctcgagga aacttttcgg agattcgttg acgaaggtga tgccgccgcg ctcgccgggg
     5641 ggctgggaat gagagaagac gtagttcttc atttttgagt cgctgaagcc gcccccgccc
     5701 atcttgaggg ccacgtcgta ggttttgcgc cccatgatgg cggtatcgat ggtggcgaaa
     5761 aacgggccca tcgaatagtc cttgggcatg aagagaaaat cgaccgctcc atcggggcgg
     5821 gcgatgtagc cgtcgaggct gatgccgagg ccaaggacga ccttgcgcat gggggcattc
     5881 taaacgacgg attgtgtatt gttcaacggt tgtgaccggc ggatcggccg cgattgaggg
     5941 aagcttgcag ccggcgcgaa aaggattgcg cgggagtttc gacttgggct aacgtgcgcc
     6001 tcggcacgaa tgcgttgatt gcccacagcg gaggaatgcg tcatgcagaa gttggtactt
     6061 gcggcggtcg gtacggtttt gctgtcgtgc ggggcatatg ggcagaatgc gccgtcagga
     6121 acgccatctt gtgagggtct ggcgcaactc gaattgtctg gcgcgaagat cctctccgcg
     6181 cggacggtgg cggccggagc gttcacgccg ccgtcaaaca tgactccctg gttggtgggg
     6241 gacgcctctt tctacaagat gctcggtgcg ttctgccggg tggtggtgac agcgactcca
     6301 agcgccgatt cctcgatcaa gatcgaggtg tggatgcccg ccaatagtca gaaggagggc
     6361 gggtggaacg ggaaactgca gggccagggc aatggaggat tcgccggaga gattggttac
     6421 caccagatgg gaatcgcggt gcaccaaggt tacgcgacgg taggcacgga taccgggcac
     6481 tccggaggcg ggactgacgc gagttgggcg ctgggacatc ccgaaaaggt gaccgacttc
     6541 ggataccgag gaatccatga aatgacgcgg gtcgccaaag cggcgatcaa ggcgttttac
     6601 ggcagcgatc cgcagcactc gtattttggg ggctgctcga acggcgggcg gcaagcattg
     6661 atggaagcgc agcgatttcc cgaagattac gacggcatac tcgcgggagc gccggccaat
     6721 tattggacgc acctgttgac caaagcgctt gcggatgcgc agacaaccac gcttgatcca
     6781 gcgagttata tccctgcgag caagctgccg gcgatcgctc gcgcggtgga cgctgcctgc
     6841 gacgcgcagg acggagtggc cgacggcatc gtgaatgatc cgaggaaatg tcatttcgat
     6901 ccggcaggga tgctttgcaa agaaggcgat gcggagaagt gcctgacggc ggcgcaggtg
     6961 accgcgttga agaaattgta cgacgggccg cacgatgcca aggagcgcga gatttttcct
     7021 ggctacgtgc cgggagcgga agagggacct ggaggatggg caacgtggat tacgggagcg
     7081 gcgccaggca agagcctgct gttcgccttc agcggcggat atttttccaa tatggtctat
     7141 gagaaggcgg actggaatta caagacaacc agcgtcgacg aagcagtgaa ggcggctgat
     7201 gagaagacgg cgcaaattct gaatgcgacg gaggcaaacc ttgtggcgtt caaggcgcgg
     7261 ggtgggaaat tgattctcta ccatggctgg aacgatccgg caatttcggc gctcaacacg
     7321 attaactatt acaacgacgt gactaacagg atgcgtgggc aggagccaga ggcgttcgtg
     7381 cggctctaca tggtgccggg gatgcagcac tgcggtggtg gaaccggacc agactcgttt
     7441 gggcagggcg gcgcgggggc gaaggatgcg cagcataatg tagagttggc gcttgaacag
     7501 tgggtggaga aaggaattgc gcccaatgct atcgtcgcga cgaagtacga aggcggcgac
     7561 ccagctaagg gcgcgaagat gacccggccg ctctgtccgt atccgcagat ggcgaagtac
     7621 aagggcaaag gcgatacgaa cgacgcgggc aattttgtgt gctatgcgga gagcaagtga
     7681 gcgaaaagga tggcgtggga gtttcgcggt ggtctaaggt gcgttgcgag acgaagacgg
     7741 ttaaaggttg aatcagagga ggagcgaaca tgtcacggaa aagcatggtg aagattttcg
     7801 ttgtctcgct cgtagtgttg ggtgcgctcg gcagcgcagt ggcgcagatg ccgaatccat
     7861 acggaccggc catcagcctg gaaaatgcca agaaggcggc tgcaccggcg ttggcggagg
     7921 cggcgaggaa taattggacc atggcggtgg cgatcgtcgg cccggcgggc acgcttgtgt
     7981 attttgagaa aatggacaac acgcagatcg gcagcgccga agtggcaatt gataaggcgc
     8041 ggtcggcggc actgttcaag cgtccgacga aggcgtttca ggatgcggtg gcggccggag
     8101 gagacggcct gcgtgtgctg ggcctgaaag gtgcggtgcc gattgagggc ggtattccac
     8161 tggtgctgga tggaaggatt gtgggcgcga ttggcgttag cggtgggacg agcgcgcagg
     8221 atgggcagtg tgccaaggcc ggcgcggaca cggtgaagta ggctccgccg atgacagagc
     8281 atggctgaaa agacggttta cctttcgctg ggatcaaacg ttggggatag agaggagaat
     8341 ttgcgcgcgg cgctcgcggc gctggaagat gcgggtgtgc gcgtaacgca ggtgtcgtcg
     8401 ttttacgaga cggagccggt ggattttctg gagcaggcgt ggttcttgaa ttgtgcagta
     8461 gagggaaaga cggacgttcc ggcgctggaa ttgttgcgga cgctgcgagg aatcgagacg
     8521 cgaatgggga gcaagaagct ggtcgcgaaa gggccgcggc tgattgatat ggacatcctg
     8581 ctttacggct cggagacgat tgacacgccg gaattgcagg tgccgcaccc gagaatgcat
     8641 ctgcggcggt ttgtgctagt gccactggcg gagattgcgg cggaggtgag gcacccgagt
     8701 ttgataaaga ctgtgaaaca gatgctcgag ggaacgccgg atcacagcac agtagagcgt
     8761 tttgatcctg ccaagtaaag gcgcagcatg ctgcgcccct acaagtgcgg acttcctgat
     8821 cgcgctaact ggtgcgatgc aagagcaagc ggtgaagggt catggctttg ggttggaggg
     8881 cgtcgagctc gatttgggcg agctggcctt ccttgatttc gcgccagcgg tagacgccgg
     8941 gctcggtttc ctgggtttca cggccgttca agcggcgcaa gaactcgttg gcgttgtcgc
     9001 gcgaagcggc ttggcggtac gtgggacggg tgagggcttc gacggcgtaa ctgtgcagca
     9061 gtttgcgcga gtagtgatcg aagagatcgc cggaagcaaa gatgtcgctc cacgcgactt
     9121 cgccgccgta ggccacgacc acgccaacga cgcgctcatt cttcaaacct gaagtggctt
     9181 gcacgaaacg gcgctggact tcgtcaacga atccatcgat ggagacgccg acggcacgcg
     9241 actcgtaaat tttttcgtag gcttcggtgc ggccgttgcc agcgatggca gattggagat
     9301 tctcggcgga tatttgagcg cgcggagcag ctggcggcgc cttcgcgttg gtgccgttgc
     9361 gaacggagta ccagactttg gtctgggcct gatcaaccgc gacgctttcg cggacactgg
     9421 ggtgaacgat ggtattcgcg gccgcgaatt gcgcgctgcc cgtccaacgg ccgtgctcga
     9481 cgcagaagac atccaggggg agcggaggtg cgccggcggg aatgatgcgg tccttgccga
     9541 tgacgcggtc ctgcttgccg ccgctgacca gttcgcccgc gaggagcaat agcgggcggc
     9601 gactgcgatt gatcaggaca agctggttga cggaagcgcc ggtaccggat tgtggaacgt
     9661 agacggggcg gccatcgcga ccgcgaacca tggtttcgga accttgctca cggacggtca
     9721 cttcgccggt ggcgagacct tcctcgagag tgaggaaggg actggtgtcc tggctgaagg
     9781 agctgaccac cgggaatacg gagatgtttt cgtaggtgac gggatcgagg agccgccagt
     9841 caccctcagg ccgagaactg ggagcggctg gggcgcccgg cactgagccg acaaaagcca
     9901 gaccgccaag tgcagtgata ccaagaagtc cgccgattag ggcggcgaaa atcgaacgtt
     9961 ccatgaaagc ctcctgtgcg tgattccctg tccggtcaga gcgaaccagc gaggacacga
    10021 ctcagcatta gaacggggag gcggcgaatt cttgcagaga ggtagggaat agaggcaggc
    10081 atcgaggaaa acaagaatac taaggcggag acacagtggg cgcagagcaa gagggagaga
    10141 cactaaacag gtaaggggcc gctcggtgtc agcggttggg gtcgccgagg ttgacgcggg
    10201 gcttggcacg cttggagctg gaggcttcgc tttcctgttt ctcgtattcg gcgagttcct
    10261 cgtcggtgta ggaaaaagag tcgcgcgagg cgaacatcga gttggagcgg ccggcttgtt
    10321 tctttttctt gcgcagagcg ccgaattcgt ggcggacctg acggatagga ccctcgatgg
    10381 cttttacttc gacgagcaga tcgctgactt tttcgagaaa gtcgtgaaga tggtagggac
    10441 gaagcacata ggggaggcgc gcggcttcca gaaggccgat gtactggccg ccggctagct
    10501 ccgggatgag gaacaagacg cgaatgcggc caccatcatc gagagagacg ctggcaagct
    10561 cgcgcagagt gacgaacgcg ggaccttcga ttccagttag agccacatcg gcaatgacaa
    10621 gagaccattc cccggttttc agctcggcca ggagcatctt ggtgtcgggg acgatgcgga
    10681 cgcgccatcc ctcggagtcc aggatctgct tgaccgcact tccaccgggg gcatcgttgt
    10741 caaggacaag aatgccgacc tgctgagggg cgggagtggg actagtactc acaatactta
    10801 gtatacggcc agaggcgcag tgacgggtgt ggaatcaagt ggttgcggcg tatcggataa
    10861 cggcggctcg aggacttcgt tctcgttcgc aagtatgccg ctactgaccg accacgaggc
    10921 cacttccaga ggtgtatttt tggaccccag tgccatccca gaagatacgg aacgtagcgg
    10981 cggggctccc tgaaatcgag cgtgtgtaga agacctggag cccatcacga gaccattcag
    11041 cgccacggct ccattggtca ggcgggcaga gagtcacgcg gcgcttcgaa cgcaattcga
    11101 agaggaagaa accagcggcc aagcccatgg agtcaaccgg agttcctaca ggcgccgttg
    11161 cgtaatcggc ggaaacaagc agcaggtctg gattgatggg gtgcactcga atcgtgtctg
    11221 agctgatgct ctgaaacgtg gcaccgtaga tgtctttgag agccacggct tgcggcaaga
    11281 cttcttcgct caaccagtag gcgttcagca tgtccgacgc caggagggtg cgattgtcaa
    11341 tccagccgtg aagagagttt acgggctggg gagaaaagga gatggccact tctggcgttg
    11401 ctacggggaa ggaccagacc tgccatgagc gatccagagc tttcagaaag gcaatgcggg
    11461 cgtcatcggg cgaccagaac ggttgacgca cgaggccgtg caccagatcc acggacttgc
    11521 cggtgtccaa atcgtagaga acgatagtgc gatccggtcc ggtcgtcgaa tcggagcgca
    11581 catagagaag cttcttgccg ctgtgggaaa gggccagttc ggtgccaact tgggcagcaa
    11641 tctttttcac ggctccgcgg ctgacgacca catttccggc tgcgttcaag taggccacaa
    11701 caaccggagg cttgaagttg gtgatggttg gggcgggggg cttctttttg gcggcgtcct
    11761 cctcgtcgtc gggttggttg ttgatcttgg ggaagagctc ggcggcgatg cgagcacagc
    11821 ccttcagggt gccgagctga gcgtgatcga cggacgcgcg gaaggggtac ttgacggccg
    11881 gtggcggcgt ggggattagc atgtctttgg catcggtgca ggcttctcgt gagaggtgca
    11941 cagcgacggc ggcgccggtg gcggagtcct tggcgtgata ggtccaggaa tcggcggcgg
    12001 cttcgtggct gacatcggtg ggttcaagag agattgacgg atacccgggg cgatcgaggc
    12061 gttccgggcc cttctggccg atgcgcaggc tccagttggg atcattacca aaggcaagaa
    12121 ggagaatggg atagtgggtc gattgagggg cggggcgacc cgtgggaagg ttctttgcca
    12181 gcggaggatt ctggccgagg gagagcatag gcaggcaaac gagaattgcg aatgctaaga
    12241 ccagagcgcg tttggctttc tcaggcatgc cgatgacagt tctcccgggc attgagaata
    12301 ccccctgaga gcgaagtagg caattgtccg cagtgcttcg caaagaggcg tcgtcgccaa
    12361 ggaggtccgc tggaataggg ggatgcgagg gcgaatctta tacgaataaa aagcgaacat
    12421 agtgcttgac taagcgaata aaaagcgaaa tactgcggcg tgcttcgagg tgggctatgt
    12481 ctccagttca ctccccatcg gcgccgagtt ttcggattcg gcgtcgccgc gtttcaccaa
    12541 ccacagtagg actctttccc gagtgccctg atgtcatagg cccaataaaa caggttcgtg
    12601 accctgcccc ggttgcggac caattgactg gcgaccgtaa gacggtcgac cgcggcaaca
    12661 tttcggatca agcgacggcg gattgccccc cggcgtccag gacccagcac ggaacccaaa
    12721 accgtgaatc gggtccggtc cgaaatgatg ccgcggcgcc ccctgaacgc gctcacgcgc
    12781 cactggttgg cattgcccgc ctcgtagcat cctccccgcc cgcggagacg agacggaagt
    12841 ccgccgagaa gccggaatat tttttgttgc ccgtgaagtc gatcctgaac cggtgcgact
    12901 cgaaccgcgt gccgttcgaa tggacgatca atccctatcg aggatgcgaa tttgcttgca
    12961 agtattgcta cgcgcggtac acgcacgaat acatggagct ggacggctcc gagttcgaga
    13021 agaagattta tgtgaagaag gatgcggcgc cgctgctggc gtgggatgtg gcgcacaaat
    13081 attcgtactc gtcggaagcg tctggcgggg cgcggccgga ccacatcgcc attggcacgg
    13141 caacggaccc gtatcagccg gctgagcgtg aatacggggt gacgcgcgct tgcttggagg
    13201 agctggctaa gagggaaggg ctgagcgttt cgatcatcac caagtcaaac cagatcgtgc
    13261 gggacattga tgtgctgcag cggatcgcgg agcggtcaaa cctgggaatc gacattacga
    13321 tcacgacgct gcgggccggg ttgacccgac tactcgagcc gcgtgcgccg cggccggatt
    13381 tgcgtctggc agcggtgaag caattgcgcg aagcagggct ggcggtggga gtgtcagcgt
    13441 caccgctgat ccccggaatc acggaccgcg atggagactt ggaggcggtg gctgcggcgg
    13501 cgaaagaggc cggtgcgcag tggttctttt cgggcgtgct gttcttgatg ccgtcgtcgg
    13561 cgagacaatt tctgccgttc gtgcgggaga agtttccccg gctggcgagg cagtatgaag
    13621 agtggtacgc gaagaatggg tacgcaccgg aagagtaccg gaagaaggct tcgcagcgcg
    13681 tggcgaggat acgggagaag tatggttttg cggcgcggcc atgggtggag gctaagagga
    13741 aggcgccgtg ctcgcagatg tcgctgagtt gggatgcggg gttggcggcg ccgccgggtg
    13801 gaatgtttgt tcggagtgct attggcgaac cagaagtgag ggccgcagct gggcgttgaa
    13861 gttgcggagc agaagaaaat acgccggcag ggatgccggc gctacgagac taagggacga
    13921 tgatgcggga ttcctcgcgt ttgagttcgg gggcatgcgg gaattggccg cgaaggaatt
    13981 cgatgaaacg gcgtgagcgt gggcggccgt tggtgcgcag gaggcccatg cggtagagaa
    14041 aaacccacag atagaaaagg tccatgtcct tgagtgcggc cgcgccagtc cgttcggctt
    14101 gctgtttctt gacttcggaa atcaaggcga tcaatgcggc atagagttcg cgcggcagcg
    14161 gcgcgtccgg tggtttttcg taaataatgc cggagcttag agtcttgtag gttt
//
LOCUS       DASYVO010000237         3270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_230407, whole genome shotgun sequence.
ACCESSION   DASYVO010000237 DASYVO010000000
VERSION     DASYVO010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230407"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..1316
                     /gene="selD"
                     /locus_tag="VGR03_07985"
     CDS             264..1316
                     /gene="selD"
                     /locus_tag="VGR03_07985"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:282..284,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEV1994254.1"
                     /translation="MVKAAGUAAKLSPGLLDSILRRLPRPTDPNVLVGFDTNDDAGVY
                     LLGEGLALVQTVDFFTPIVDDPHTFGQIAAANALSDVYAMGGKPVSALSIVGFPDKGE
                     PAILEQIILGGLSKMSEAKCSVIGGHSIRNDDIQFGYAVTGVINPHRVWRNVDARAGD
                     VLLLTKAIGTGILSTALKKEHVGPVALQAAVASMTELNRAASEALQELEEKAGPTRPV
                     HAVTDVTGFGLLGHAREMALGDPARGIEAVSLEIDHSAILYLPGAVEAAREGFLPGGL
                     KNNRDFIGDCVGFAEGVPQEYRDLLFDPQTSGGLLISVSPESADAAVSALDRHSVSAR
                     RVGRVVAKKNPLLFVQ"
     gene            1354..2547
                     /locus_tag="VGR03_07990"
     CDS             1354..2547
                     /locus_tag="VGR03_07990"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEV1994255.1"
                     /translation="MDTITHGIAGALIGKAVFRGEDMFASHPMNRGRVITWSLMLGAI
                     FPDSDVLRDFFSNDKLLIVTWHRSITHSLVMLPLWALLLAALTRAIAKWRKWEAPSFA
                     ATSAIYAIGILSHILLDLVTSFGTMIWSPLRWSRPAWDLIFIVDFTLTAIFLVPQLLA
                     WVYTHPKKVKGRAVGMWLVFLPAPFLIGRIGRNVGAPISDRVVLSVVVILAALFLLPT
                     LRGWGLKIHHVTWNRAGFFAALVYVALATYAHHTALTRIQKFAELDHLQVESIGALPL
                     PPSLWHWDGLVRTQRGVYELRMDLSDKPVSDTELLTREHSYFPDAYPNSYIDAAKRLS
                     EVQKVLWFARFPVTRFHKEGDAAIVEISDIRFVQTRRDRPPPFTYRVRFAADGNVVSQ
                     GWVTR"
     gene            complement(2591..3121)
                     /locus_tag="VGR03_07995"
     CDS             complement(2591..3121)
                     /locus_tag="VGR03_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEV1994256.1"
                     /translation="MAQELVHDGKLDRQATADALRHFLENTLRASGLELKVNTRALSA
                     DASGTEGSAEVLADLDGKDKEILLERGAEVLKALEHLAFKALRLEPTYHEKIHIDSGG
                     YRALRFEELRMTARVAAERVQTSRQPFRLNPMSSRERRIVHLALKDMTGVRTESIGVG
                     EERQVVIHPADSKPSR"
BASE COUNT          617 a          987 c          929 g          737 t
ORIGIN      
        1 agaaactggt gaaggcgcgg gtcgcccaga acgaaagcaa gaaacggcgc tgaccaggtt
       61 gccctcgaga ccggttcatg acttccaaga cggttgactg ccccgtcacc atctaaatgg
      121 actcaacgcc tggttaccgc attaaccgga aattgatgca gtaacaactg caatggcgca
      181 caagcaagaa ccgcggcacc tcgctataat cgaaacgctt tctagcgagg tgcggcttga
      241 cccaatccgt gaagcttacg gccatggtca aggcagctgg ttgagcggcc aagctgagtc
      301 cagggttgct ggattccatt ttgcgccgtt tgccgcggcc aactgacccg aacgtgctcg
      361 ttggctttga caccaacgac gacgcggggg tgtatctact cggcgaagga ttggcgctgg
      421 tgcaaacggt ggatttcttc acgcccatcg tcgacgaccc gcataccttc ggacagattg
      481 cggcggcgaa cgctctgagc gatgtctacg ccatgggcgg caagccggtt tccgcgctct
      541 cgatcgtggg cttccctgac aagggcgagc ccgctattct cgagcaaatt attctgggtg
      601 gcctttccaa aatgtccgag gcgaaatgca gcgtcatcgg cggccactcg attcgcaacg
      661 acgatattca attcggctac gcggtgactg gcgtgatcaa cccacatcga gtttggcgaa
      721 atgtcgacgc gcgcgcaggc gacgttttgc tgctgacgaa agccattgga acgggcatcc
      781 tctcgacggc gctcaagaaa gaacacgtag gccctgtagc cctgcaggcg gctgttgcct
      841 ccatgaccga actgaatcgc gcggcctccg aggcattgca agagttggaa gaaaaggcgg
      901 gacccacgcg gccggtccac gcggtgacgg acgttactgg atttggcctg ctgggccatg
      961 cgcgggaaat ggccctcggc gatccggcgc gtggaatcga agccgtgtct ctcgagatcg
     1021 atcattcggc tatcctttat cttcccggag ccgtggaagc cgcgcgcgaa ggcttcctcc
     1081 ccggcggcct gaagaataac cgcgatttca ttggcgattg cgtgggtttc gccgaaggcg
     1141 ttccgcagga ataccgcgac cttctcttcg atccacagac ttccggcggc ctgctcatct
     1201 ccgtctcgcc cgaatctgcc gacgcggccg tttccgctct ggatcgccat agcgtttctg
     1261 ctcgccgcgt cggccgcgtc gtcgccaaaa agaatccgct tcttttcgtc cagtagaatt
     1321 caggatgccg agcgcatccg atagacttca ttcatggaca cgatcacaca cggcattgcc
     1381 ggcgcattga ttggaaaagc cgtctttcgc ggcgaggaca tgttcgcctc gcatcctatg
     1441 aaccgcggcc gcgtcatcac ctggtcactg atgctcggcg ccattttccc ggactccgac
     1501 gtgctccggg actttttctc caacgataaa cttctcatcg tcacctggca ccggagcatc
     1561 acgcattcgc tggtgatgct tccgctgtgg gctctgctgc ttgcagcctt aacgcgagcg
     1621 attgccaagt ggcgaaaatg ggaagcgccc tcatttgccg caacgagtgc gatttacgcg
     1681 atcggcatat tgagtcacat cctgctcgac ctggtcacgt cattcggcac catgatctgg
     1741 tctccgctgc gatggtcgcg cccggcctgg gatctcatct tcatcgtgga tttcacgctg
     1801 accgcgattt ttctcgtgcc gcaattgctc gcctgggttt atacccaccc caaaaaagtg
     1861 aagggacggg ccgtcggaat gtggctggtt tttctgcctg caccatttct catcggcaga
     1921 atcggtcgaa atgttggcgc gccgatctcg gatcgtgtcg tcttgagcgt cgtggttatt
     1981 cttgccgcgc tcttcctctt gcctacgctc cgtggctggg gcttgaagat tcaccacgtt
     2041 acctggaacc gtgcgggttt cttcgccgcg ctggtttatg tggcgctcgc gacctatgcg
     2101 catcacaccg ctctcacgcg cattcaaaag ttcgcggaat tggatcattt gcaggtcgag
     2161 tcgatcggcg cgttaccgct tccgccatcc ctttggcatt gggacggact ggtccgcaca
     2221 cagcggggcg tctatgagct gcgcatggac ctaagcgata agcccgttag cgacacggaa
     2281 ttgcttacgc gtgagcacag ttattttccc gatgcctacc cgaattccta tatcgatgcc
     2341 gccaaacgtt tgtccgaagt ccagaaagtc ctatggttcg cgcgattccc ggtcacgcgc
     2401 ttccacaaag aaggcgacgc agccatcgtg gaaatttctg acatacgatt tgtacaaacg
     2461 cgccgcgacc gccccccgcc cttcacgtat cgcgtgcgtt tcgctgccga tggaaatgtg
     2521 gtctcacaag gctgggtcac gaggtaggac aggcattctt gcctgtcctt cttcttgtgg
     2581 caaagtggaa ctatcgggaa ggtttgctgt ctgccggatg aataacgact tggcgctctt
     2641 cgcccacgcc gatgctctcg gtgcgtactc cggtcatgtc cttcagcgcc agatgcacga
     2701 tgcggcgctc gcgcgaggac atgggattca gccgaaacgg ctggcgggaa gtctgcacgc
     2761 gttccgccgc gacgcgcgcc gtcatccgca gttcttcgaa gcgcagcgcg cggtaaccgc
     2821 cagaatcgat gtgaattttt tcgtggtagg tgggttccaa tcgcagcgcc ttgaacgcta
     2881 gatgctccag cgctttcaac acttccgctc cgcgctccag cagaatctct ttgtccttgc
     2941 cgtcgagatc ggcgagcact tccgcgctgc cctcggttcc gcttgcatcc gcggataagg
     3001 cgcgcgtgtt caccttcaat tcgagtccgg aagcccgcag cgtgttttcc agaaaatggc
     3061 gcaaagcatc ggcggttgct tgccggtcca gcttcccatc gtgaacgagt tcttgagcca
     3121 tagggcgttc gaacggaacg agcctcgcca gcttctcgga aagaatcttt ccttaagact
     3181 tcttcccgcg cacgggcttt gctggtgcgg gcgtgggatg cttgcgattc aaataccact
     3241 gctgcgcgat gcccacgacg ctgctggtga
//
LOCUS       DASYVO010000238         1565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_274602, whole genome shotgun sequence.
ACCESSION   DASYVO010000238 DASYVO010000000
VERSION     DASYVO010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274602"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /gene="cmk"
                     /locus_tag="VGR03_08000"
     CDS             <1..594
                     /gene="cmk"
                     /locus_tag="VGR03_08000"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV1994257.1"
                     /translation="FKAIRRGVSISNEAQLEALARETHIELRAPTPAHEEAGAKNCVF
                     LDGTDVTKEIRTAEVAQTASRLATIAKVRELLVAEQRRAGAGGGVVMEGRDIGTVVFP
                     DAELKIFLDASPEERAERRWKEHQEKGEKLTLAEVFDEVVERDKRDRERKVSPLVRAA
                     DAVLVDNTAMGIEETARLIVMLARERERELKEAGRTG"
     gene            591..>1565
                     /locus_tag="VGR03_08005"
     CDS             591..>1565
                     /locus_tag="VGR03_08005"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEV1994258.1"
                     /translation="MMRLFAMLAIGAGSALFVMPMLALAQPSSSANASPAEATRKFRA
                     YLDEDWKRWMEEYPEMATSAGYPGQNRRWGDDSAKGMEARIKHLYGSMTLLKTVSREA
                     LPVEEQLNYDLYRNLLETAEEGLQYGDDPMPFHSVVPLNMWMPMTQMGGIQQGAAATI
                     ATMPHQTVVDYDDILTRLEALPKNVEQNLLFLQGGLKKGYTPPKLMLRDVPKQIADLI
                     PADPLASPLLEPFRKFPAGFPGAERSRLTERAKTIYTMGVAPAFQKLHDYVASAYLPA
                     CRENIAATALPNGAAAYAFHVRWQTTTNLTPQQIHEIGLAEVKRIRAEM"
BASE COUNT          368 a          406 c          518 g          273 t
ORIGIN      
        1 tttaaagcca ttcggcgagg agtgtcgatt agcaatgaag cgcaactcga agcgctcgcg
       61 cgcgagacgc acatcgagct gcgggccccc actcccgcgc acgaagaagc cggcgcgaag
      121 aactgcgtgt ttctggatgg aacggacgta acgaaggaaa ttcgtacggc cgaggtggcg
      181 cagacggcgt cgcggctcgc gacgatcgcc aaagtgcgtg agttgctggt tgcggaacag
      241 cggcgcgcgg gagcgggtgg cggcgtggta atggaagggc gcgacatcgg cacagtcgtt
      301 tttccagacg ccgagctcaa gattttcttg gatgcttcgc cggaagagcg ggcggagcgg
      361 cgatggaaag agcaccagga gaaaggcgaa aagttgacgc tcgcggaagt cttcgacgaa
      421 gtggtagagc gagacaagcg cgatcgcgag cgcaaagttt ccccgctcgt gcgcgcggcg
      481 gatgcggtgc tggtcgacaa cacggcgatg gggatcgagg aaacagcgcg gctgatcgtg
      541 atgctggcgc gggagcgcga aagggaattg aaggaagcag ggaggacggg atgatgcgtt
      601 tgtttgcgat gctggcgatt ggtgcgggga gtgcgctgtt cgtaatgccg atgctggcgt
      661 tggcacagcc cagttcgagt gcgaacgcca gcccggcaga agctacccga aaattccgcg
      721 catacctcga tgaagactgg aagcgctgga tggaggaata ccctgaaatg gccacctcgg
      781 ccggatatcc cgggcagaac cgccgctggg gcgatgattc ggcgaaaggt atggaagcac
      841 gaatcaagca tctgtacgga agtatgactc tgctgaaaac agtttcccgc gaggctcttc
      901 ccgtagaaga acagctcaac tacgacctat accggaattt gctggaaaca gcggaagagg
      961 gtttgcagta tggcgacgac ccaatgccgt ttcatagcgt cgtaccgctc aatatgtgga
     1021 tgccgatgac ccagatggga ggaatccagc aaggcgcggc ggcaacgatc gcaacgatgc
     1081 cgcaccagac ggtggtggat tacgatgaca ttctcacacg gctcgaagcg ttgccgaaaa
     1141 atgttgaaca gaatcttttg tttcttcagg ggggtttgaa aaaaggctac acgccgccga
     1201 agttgatgct gcgcgacgtt ccgaaacaga tcgctgattt gattcccgcc gatccgctgg
     1261 cgagcccact gctggagccc ttcaggaaat ttccagcggg attcccaggg gccgaacgtt
     1321 cacgattgac ggagcgagcc aagacaatct acacaatggg agtcgcgccg gcgtttcaga
     1381 aactgcatga ctacgtagct tcggcgtatt tgcctgcgtg ccgcgaaaac attgcagcga
     1441 ccgccctgcc gaacggcgcg gcggcgtatg cgtttcacgt gcgctggcag accaccacaa
     1501 acctgacgcc gcagcaaatc catgaaatcg gactcgccga agtgaagcgc atccgtgcgg
     1561 agatg
//
LOCUS       DASYVO010000239         1736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_144753, whole genome shotgun sequence.
ACCESSION   DASYVO010000239 DASYVO010000000
VERSION     DASYVO010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144753"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1698
                     /locus_tag="VGR03_08010"
     CDS             <1..1698
                     /locus_tag="VGR03_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1994259.1"
                     /translation="VYAYARELDDWIKKRQPKDDPDADDAFVREQELSGTDSPINVGD
                     APAEHPTPDPAPEPPKPPPSAGKRGVVAMFALAILVGLSFGVYRWLQARDSIRDKVRL
                     AVLPFTYVSGDSKPDYITAGLTDVIRTKLGQLDPLHLGVIAATSSKIVSGKPITEIGR
                     ILNVKYVLEGSVQRAGNQVRIDVQLIQVSDETHLWADSFTRELSDVLQVESDVSAAIA
                     RQILATLPVPPSPQTQPSLRPGTVHAATPEIANSRHAYHQGKFAWGSRGDLRSSIVFF
                     EQAIHDDPTYAEAYAGLAAATAILGQVPNDGMPPGEAKPKAREAAQRALQLNPRLAEA
                     HAVLGNVAMSYDWDLVTGEKELLRAIELNPNDPNAHEWYCHLLIVEGRNPEALTEARH
                     ALDLDPVSPLFHTVLAETYYFGRAFDAAIEEAQQVVKLHPDFLPAHYWLGSAYREKKM
                     YPQAMETFQHARRLSGDNPAMVMAYGHAQALAGNAVEARAALQKLQQLAATRFVPNLY
                     PAAIHVGLGETDEAFRLLELAYQERIDRLVYLNVDPMADPIRSDPRFAQLMAKVGFH"
BASE COUNT          352 a          591 c          456 g          337 t
ORIGIN      
        1 gtttacgcct acgcgcgcga actcgacgat tggatcaaga agcgccagcc gaaggacgat
       61 cccgacgccg acgacgcctt tgtccgcgaa caggaattga gcggcacgga ttctccaatt
      121 aacgtcggcg atgcgccggc tgaacatccc actcccgatc ccgctccaga gccgcccaag
      181 cctcctcctt cagcagggaa gcgcggcgtc gtcgccatgt tcgctctcgc gattcttgtt
      241 ggtctctcct ttggcgttta ccgctggttg caggctagag attccattcg cgacaaagtt
      301 cgcctcgccg tcctgccctt cacctacgta agcggcgatt ccaagccgga ctacatcacc
      361 gccggcctta ccgatgtcat tcgcaccaaa ctcggccagc tcgatcctct gcacctcggc
      421 gtgatcgctg cgacctcctc aaaaattgtc tccggcaaac ctatcaccga aatcggccgc
      481 atcctcaatg tgaagtatgt gctagaaggc agcgtgcaac gcgccggcaa ccaggtgcgc
      541 atcgacgtcc aattgatcca ggtcagcgat gagacgcatc tttgggccga ttctttcact
      601 cgcgaacttt ccgatgtcct gcaagtggaa tcggatgtct ccgccgcgat cgcccgccag
      661 atactcgcca ctcttcccgt gccgccgagt ccgcaaactc agccctcgct gcggcctgga
      721 accgtccacg ccgcgacacc ggagatcgcc aactcccgcc acgcttacca tcagggcaag
      781 ttcgcgtggg gcagccgcgg cgatttgcgc agcagcatcg tatttttcga gcaggccatt
      841 cacgacgatc cgacttacgc ggaagcctac gccggcctcg ccgccgccac tgccattctc
      901 gggcaggtgc ctaacgacgg aatgccaccc ggcgaggcca aaccaaaagc gcgggaggcc
      961 gcacagcggg ccttgcagct caatccccgg cttgcggagg ctcatgccgt ccttggcaac
     1021 gtggccatga gttacgactg ggacctcgtc accggcgaaa aagaattgct ccgcgccatc
     1081 gagctcaatc ccaatgatcc caacgcccat gaatggtatt gccatctcct gatcgtcgaa
     1141 ggtcgcaatc cagaggcatt gaccgaagcg cgtcacgctc tcgatctgga ccccgtttcg
     1201 cctctcttcc acaccgtgct tgccgaaaca tattacttcg gccgcgcgtt tgacgctgct
     1261 atcgaagaag cacagcaggt cgtaaagctc catcccgatt tcctgccggc gcattactgg
     1321 ctcggctcgg cctatcgcga gaagaaaatg tatcctcagg ccatggagac ttttcagcac
     1381 gcgcgccggc tctccggaga caatccggcg atggtcatgg cttacggcca cgcccaggcc
     1441 ctggcaggaa atgccgtgga agcgcgagcg gcccttcaga agctgcagca actggcggcc
     1501 actcgattcg ttcccaactt gtacccagcg gccatccacg tcggactggg cgaaacggat
     1561 gaagcctttc gcttgctcga actcgcctat caggaacgca tcgaccgact cgtctacttg
     1621 aacgtcgatc ccatggccga ccccatccgt tccgatcctc gttttgccca gctcatggcc
     1681 aaggtcggat tccattagca aaaaggctgt caatcagcag ggaagtggcc tgcaga
//
LOCUS       DASYVO010000240         1514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_34476, whole genome shotgun sequence.
ACCESSION   DASYVO010000240 DASYVO010000000
VERSION     DASYVO010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34476"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..464)
                     /locus_tag="VGR03_08015"
     CDS             complement(234..464)
                     /locus_tag="VGR03_08015"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEV1994260.1"
                     /translation="MAEKLPVLKAKEVLRALLRAGFYVHHQTGSHARILHETNSELRV
                     TVPMHARDIPPVLLKRILKQASLSEDDFRKYL"
     gene            complement(480..746)
                     /locus_tag="VGR03_08020"
     CDS             complement(480..746)
                     /locus_tag="VGR03_08020"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV1994261.1"
                     /translation="MNSSLSTKEKHASAEYSYSVIFQPFAEGGYEVLVPAIPEICTFG
                     ATLPEARRMAKDAIRCFLESALKTGEPIPKDLEPATEHVAVVLP"
     gene            901..>1514
                     /locus_tag="VGR03_08025"
     CDS             901..>1514
                     /locus_tag="VGR03_08025"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV1994262.1"
                     /translation="MIGETISHYRILEKLGGGGMGVVYKAEDVRLGRSVALKFLPAEL
                     ARDHQALERFQREARAASALDHPHICTIYDIGEHDGEPFIVMQYLQGQTLKHRLAAGR
                     LKVEVILDLGVEIADALQAAHSKGIVHRDIKPANIFITERGEAKILDFGLAKLAPPPG
                     ACPGEPLRAEGATAVTMATAEEHLTSPGTAMGTVAYMSPEQARG"
BASE COUNT          326 a          429 c          426 g          333 t
ORIGIN      
        1 cgcggccgct aaatcgccca ccactttaaa agcaaactgc atcatcgcat cttgattgac
       61 tgctggcact actgctgctt tcgtgcccat cttccgcctc ctattctgat tattgagaag
      121 tgcgaacaat actacggcga aatgatatga cggttgtcgg gttgtttcaa tcccgattag
      181 gtattcaaca gcttgtatcg cctaatctga aagatattca tttggctaga aatctataag
      241 tatttcctga aatcgtcttc cgacagcgaa gcttgcttca ggatacgctt cagtaaaacg
      301 ggtggaatat ctctcgcatg catgggcacg gtcacccgga gttcagagtt ggtctcgtgt
      361 aaaattcttg cgtgactgcc ggtttggtgg tggacgtaga atcccgcgcg cagcaaggcg
      421 cgaagaactt ctttggcttt tagaaccggt agcttttcgg ccattcacca caagccagtt
      481 tagggtagga ccaccgcaac gtgctcagtg gcaggttcca aatccttcgg aataggttcg
      541 ccggttttta gcgcgctctc gagaaaacaa cgaattgcgt ccttagccat tcggcgggct
      601 tcgggaagag tggcaccgaa ggtgcagatt tccgggatcg ccggcaccag cacttcgtag
      661 ccaccctcgg cgaagggttg gaaaataaca gaatagctgt actcggccga agcatgtttt
      721 tctttggttg aaagtgaaga gttcatacag tcaaaccact ataccatgca ccggccctta
      781 gccattgtca aaatggaggg tggcgccact tgctgttagc tctttagcct ttcaggcctt
      841 gacgggctcg cgccctcgcc ctaaactgcc catacaaatc agtccgcgct cgcgctcgtt
      901 atgattggcg aaaccatttc tcattaccgg atcttagaaa agctgggtgg cggcggcatg
      961 ggcgtcgtgt acaaggcgga agacgtccgc ctgggccgca gtgtggcgct gaagttcctg
     1021 cccgcggagc tggcgcgtga ccatcaagcc ctggaacgat ttcagcggga ggcgcgcgcc
     1081 gcttccgccc tcgaccatcc tcacatctgc acgatttatg atatcggcga gcacgacggc
     1141 gagcccttca tcgtcatgca atacctgcaa gggcaaacgc tcaagcaccg tctcgcggcg
     1201 gggcgcttaa aggtggaggt gattctcgat ctgggcgtcg agatcgcgga cgcgctgcaa
     1261 gcggcgcact cgaagggcat cgtgcaccgt gacatcaagc ccgccaacat tttcatcacc
     1321 gagcgtggcg aggcgaagat tttagatttc ggtctggcca aactcgcgcc gccacccggg
     1381 gcgtgccccg gcgagcccct acgtgccgaa ggcgcgaccg ccgtcaccat ggcgacggcc
     1441 gaggagcacc tcaccagtcc gggaacggcg atgggcacgg tcgcctacat gtcgccggag
     1501 caggcgcgcg gcga
//
LOCUS       DASYVO010000241         3284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_75630, whole genome shotgun sequence.
ACCESSION   DASYVO010000241 DASYVO010000000
VERSION     DASYVO010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75630"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VGR03_08030"
     CDS             <1..220
                     /locus_tag="VGR03_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994263.1"
                     /translation="AIAAIWYERALQTPGNDPESTLALRYDLGVAQESAGEPEAALKS
                     FSQVYAMNIDYRGVAERIAALQKPIRRS"
     gene            254..499
                     /locus_tag="VGR03_08035"
     CDS             254..499
                     /locus_tag="VGR03_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994264.1"
                     /translation="MNRVYRALLVVLAFEMGAMLLYLPWSGYWEQNFFLSHYPSLMPI
                     VLHPSFRGAVSGIGVLDILLAIGWIGSRPGPDRVPPQ"
     gene            528..1175
                     /locus_tag="VGR03_08040"
     CDS             528..1175
                     /locus_tag="VGR03_08040"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1994265.1"
                     /translation="MTDRHSLSEAEVRKAQETLLLKIGSAAAAGVDWIQIREKDLSGR
                     GCSFLAREALQRAPISPGSNGAPARILVNDRLDVALSEHAGGVHLGESSLPLREAKRL
                     ANAQAQSQDFLIGVSCHSLEAARSAASDGADYLFFGPVFTTPSKAAFGAPQGIERLGD
                     VCRAVSVPVLAIGGITLANAAVCFAAGASGIAAIRLFQDAPDMSSVVYSLRKLTR"
     gene            complement(1176..1676)
                     /locus_tag="VGR03_08045"
     CDS             complement(1176..1676)
                     /locus_tag="VGR03_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994266.1"
                     /translation="MRDSLSLTPPEEATEDSAKLVLALLEEAYEKKTWHGPNLKQALK
                     GVTAKQAARRLGPARHNIWEVTLHAAYWKYAVRRRIEGGKRGSFALKGSNFFARPEKG
                     KLNEAAWRADKKLLEREHRVLRVTVAKVLRTARGAKFVSQLYGVAFHDIYHAGQIRLL
                     RRLMQR"
     gene            complement(1702..2961)
                     /locus_tag="VGR03_08050"
     CDS             complement(1702..2961)
                     /locus_tag="VGR03_08050"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEV1994267.1"
                     /translation="MPHHLVREHLLTALLESPEDGILSVSLDGTIDTWSRGAESIYGY
                     TAQEMTGQPLRRLVPLHELPELETIFSKAGGNGSTFFETTERLHKDGSRIFLKIKRVL
                     IRDEHGEVRGILESGRALNSYGADPSAEEPLRLIIEQMPGLHWTTDQNLRITSNWGKG
                     LACSKIPPDALVGRSVCEFLGCADRHTTPIAEHYEALRGCSSHFEYNWKNRFWEIRLE
                     PLRTVSGEINGCLGSGTDITDRKKNEEQVLIQARRDALTGLANYREFMDRLEQEVHRA
                     ERSHHSFTVLLLDLDGLKRINDLHGHLAGNRALQRLAAVMNEHCRSTDLAVRYGGDEF
                     AVVLIDSDKGMAEQVAQRIENGLRTDQGKPAISVTIGIGIYPDDGRTAAELIEAADQQ
                     LYKYKRTENRRAASATGRILNTKRAGH"
BASE COUNT          629 a          956 c          934 g          765 t
ORIGIN      
        1 ggccatcgct gccatctggt atgagcgcgc cctgcaaacc cccggcaatg atccggaaag
       61 cacgctcgcc ttgcggtatg atctgggcgt agctcaggaa tcggcgggcg agcctgaagc
      121 ggcgctgaaa agtttctctc aggtctacgc gatgaacata gattatcgcg gcgtggccga
      181 acgcatcgcg gcccttcaaa agcccatacg caggtcctga acctttcctg acaggacggg
      241 attccgctca tccatgaacc gggtttatcg cgcactgctg gtcgttcttg cgttcgagat
      301 gggcgcaatg ctcctttatc tgccgtggtc cggctattgg gaacagaatt tttttctcag
      361 tcactaccct tcgctcatgc ccatcgtgct tcatccgtcc tttcgcggcg cggttagcgg
      421 catcggcgtt ttggatattc ttttggcaat cggctggatc ggttcgcgcc cggggcctga
      481 tcgtgtccca cctcaataac tcatcttgca ggccgctcct ctgctacgtg acggaccggc
      541 acagcctctc cgaagccgaa gttcggaagg cacaggaaac tctcttgttg aagatcggat
      601 cagccgcagc cgctggcgtc gattggatac agattcggga gaaagatctt tcgggcagag
      661 gctgcagctt cctcgcgcgt gaagctttgc agcgggcccc aatatcgcca ggaagcaatg
      721 gtgctccagc acgcatcctg gtgaatgatc ggctggatgt agcgctctcg gagcacgccg
      781 gcggcgtgca tctcggcgaa agcagtctgc cgctgcggga agcaaagcgg cttgcgaacg
      841 cccaagccca aagccaggat ttcctcattg gtgtttcctg tcactccctg gaagctgcga
      901 gatccgccgc gagtgacggc gcggactatc ttttttttgg ccccgtcttc accacgccat
      961 cgaaagcggc gttcggtgca ccgcagggga ttgagaggct cggcgacgtc tgccgcgcgg
     1021 tctccgtccc ggtgctggcc atcggcggga ttacattggc gaacgccgcg gtttgcttcg
     1081 cagccggcgc atcggggatc gcggcaattc gcctttttca ggacgctccg gacatgtcat
     1141 ctgttgtgta ctccctgcga aagctgacac gctaatcagc gctgcatcag ccggcggagc
     1201 aggcgaatct gtcccgcgtg ataaatatcg tgaaacgcca cgccgtagag ctggctcacg
     1261 aattttgcac cccgcgccgt gcgcaatact ttggctaccg tcacgcgcaa gacccgatgc
     1321 tctcgctcaa gtagcttttt gtccgctctc caggccgcct cgtttaactt gcctttttcg
     1381 ggtctcgcaa aaaaattgct tcctttcaac gcgaaagaac cacgcttccc gccttcgatt
     1441 cttcgccgca cggcatactt ccagtacgcg gcatgaagcg tcacttccca gatgttgtgc
     1501 cgggccggtc cgaggcgccg tgcggcttgc tttgcagtta ctcccttgag ggcttgcttg
     1561 aggttcggtc catgccaggt ctttttctcg taggcttcct cgagcagcgc gagcacaagc
     1621 tttgcggaat cctcggtggc ctcctcaggg ggagtcagtg agagagaatc ccgcaaagga
     1681 tggcgcgctc gctctattgt gtcaatgccc ggctcttttg gtgttgagaa ttcgtcctgt
     1741 ggccgaagcc gcgcgtcgat tctctgttct cttgtacttg taaagttgct ggtccgctgc
     1801 ttcgataagt tcggcagcag tcctgccatc atccgggtag atacctatgc cgatggttac
     1861 gctgatggcc ggcttaccct gatcggttcg cagcccattc tcgatgcgct gcgcgacctg
     1921 ttccgccatc cccttgtctg agtcgatcag gacgactgca aactcatcgc cgccgtaccg
     1981 aactgccagg tccgtcgaac ggcaatgctc attcatgact gccgcgagac gctgcagcgc
     2041 gcggtttccc gccaggtggc cgtggagatc gttgattcgt ttcagcccat cgaggtccag
     2101 cagcagaact gtgaaggagt gatgactccg ttcggcgcgg tgcacttcct gctccagcct
     2161 gtccatgaat tccctgtaat ttgccaatcc cgtcagggcg tctcgccggg cctgaatgag
     2221 gacctgctct tcgtttttct tgcggtcggt gatgtccgtt cccgacccaa ggcacccgtt
     2281 gatttcgccg gacaccgtgc gcagtggttc aaggcggatt tcccaaaagc gatttttcca
     2341 gttgtattcg aaatgtgaag agcagccccg caatgcttcg taatgttctg caatgggcgt
     2401 ggtgtgccgg tcggcgcatc ccaggaattc acaaacgctg cggcccacca gtgcatcagg
     2461 gggaatcttt gaacaagcca aacccttgcc ccaattcgac gtgatccgca gattctggtc
     2521 cgttgtccaa tggagtcccg gcatctgctc gatgatcaac cggagaggct cttcggccga
     2581 cggatcggct ccgtaagagt tgagcgcccg cccgctctcg agaattcccc taacttcacc
     2641 gtgttcatcc cggatgagta cccgctttat cttcaagaaa attcttgatc catctttatg
     2701 gaggcgctct gtagtttcga aaaacgttga gccattccct cccgctttgg agaagatggt
     2761 ctccagctcc ggcaactcgt ggagcggcac gaggcgcctc aagggctgac cggtcatctc
     2821 ttgcgccgtg taaccataaa tgctctccgc acctcggctc caggtgtcga tggtgccatc
     2881 gagggatacg ctgaggattc catcctccgg cgattcgaga agagctgtaa gcaagtgttc
     2941 acggaccaga tgatgcggca ttgaaatctg accgtctttc agaaagggga aggcgttcgt
     3001 gctgagagga agtttcccgc gggcgagagg gccgggccag agggcagttc agggctgcga
     3061 acacaaccgc agctttgatt gttcaattct aatactcgaa gccgcagacg caagggataa
     3121 tcgagttcaa ctacaccaat aagtaatctt ctcggaatgg ccttgggcaa gtgaggggga
     3181 gggggaagga ggggcgaaat ccagttgaca ttgagttgca aactatatcg gactaagtta
     3241 gtctgatata gtttgcaact aatttgcaag gagacccagt ggcc
//
LOCUS       DASYVO010000242         7270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_119417, whole genome shotgun sequence.
ACCESSION   DASYVO010000242 DASYVO010000000
VERSION     DASYVO010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119417"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1957
                     /locus_tag="VGR03_08055"
     CDS             <1..1957
                     /locus_tag="VGR03_08055"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1994268.1"
                     /translation="TSPIFKPGDAEADLSATILGGGKSSRLYKKLVYEKQIAQDVAVN
                     QQSLILGSVFEVQATAKAGVKPEDLEKAINAELEAFRKEGPTAAELTRARNVIESRII
                     AGLETLGGFGGVADRLNSYNHYLGTPDFLAADIGRYESASMESIQAFAQGQLNGNQRA
                     VVYGLPGKQDLGAEVATPKAEQKDPSKNNGEPVNVDAEWRKDAPKAGPASALHLPVPE
                     KFKLSNGLTVMYSERPGLPLVAANLVLHAGTGVNPVDRPGLASMTARMLQQGTTTRSA
                     LQIADRAADLGATLNSGAGADTTGISTRSLSRNFPDALELLADVALHPSFPQAEIERV
                     RSERLTAIVQEKDEPFSLATRVLTAALYGSRHTYGYPDSGTTESIKAISRDDLEHFWK
                     QNYFPDDAALVVAGNIKLTALKPLLEKQFGAWKAGRPAATVMGSPETTDAKLILVDRP
                     GAPQTTLVCFSMGLARSTPDYAPVEVMNTDLGGLFSSRINMNLREQHGYTYGAFSFFM
                     YHRAPGPFIAGSDVRTDVTAPATTEVFNELRRMRDTQMTPAELILSKDSIARSLPGRF
                     EHGTDAAASFAELFTYDLPLDYYSTLSDRINAVTVEQAQAMAQKYILPEKMIVLAVGD
                     RSKIEEEMKKLNLGKVEIRDTDGKIVK"
     gene            1995..2846
                     /locus_tag="VGR03_08060"
     CDS             1995..2846
                     /locus_tag="VGR03_08060"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1994269.1"
                     /translation="MTANPLFLSLELARRIELAEAQAAVDCAETLDRLQPGSGAAVEE
                     IAGGFAIYCGANSPTTQAVGLGLAGPVSEEEFDGLETFYRSRGEPVRVETCPLAEASI
                     IEHFGKRCYRVTEFSSVMALPLDDANRVQSWHGPPPGMTIEKLGHDQIDLWTMTVAQG
                     FAEDAPVVPEILEVMKMFALGPSAECYLARIDGNVAGGATLAIRNGVAGLFGASTLPA
                     YRNRGVQTALLHARLSRAAEAGCDLAASLTRPGSTSQRNTMRQHFQVLYTRVKFESGF
                     ESGNLAQ"
     gene            complement(2879..3277)
                     /locus_tag="VGR03_08065"
     CDS             complement(2879..3277)
                     /locus_tag="VGR03_08065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEV1994270.1"
                     /translation="MMQKKIRVLIAKPGLDGHDRGAKIIARALRDAGMEVVYTGLRQT
                     PEMIASAAVQEDVDVIGLSILSGAHNTICPQLMKLLRDKGMTDVTVLVGGIIPEADIP
                     ALKQSGIAEIFLPGTSTQDIVEFIHKRVAH"
     gene            complement(3321..3881)
                     /locus_tag="VGR03_08070"
     CDS             complement(3321..3881)
                     /locus_tag="VGR03_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994271.1"
                     /translation="MLFLKKYLSLFVGLAVIALIVIIWLETSKITGDVSLAGSNGPAV
                     ALSGARVTLYEVTEERERLLTAALADLQQQNQQAKDANARVFPAENTDDLARSTLAGY
                     NNLSDTKHCFALEKVLDEIRKSAVRRETTDSQGHFGFRALPGRYVLEIVGQASGQYIV
                     FVEHVEPKWHSYFKLADPSCRYSLTN"
     gene            complement(3957..5675)
                     /locus_tag="VGR03_08075"
     CDS             complement(3957..5675)
                     /locus_tag="VGR03_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEV1994272.1"
                     /translation="MSEQSKGTTQAAPPLESTSADRKPSAKEQAWEKNTLQPALEKGS
                     ERQSEFTTISGYPIRRLYTKADLPDWDPERDLGFPGEPPYTRGIHSTMHRSRLWTMRQ
                     FAGFGAAEDTNQRFRYLLAQGQTGLSTAFDLPTLMGYDSDHALSEGEVGKCGVAISSL
                     ADMEVLFDKIPLANVTTSMTINSPAAVIWAMYLAVAEKQGADWKTISGTLQNDILKEY
                     IAQKEYIYPPEPSMRLVIDTFEFGVKNTPKFNPISISGYHIREAGSTAIQELAFTLRD
                     GMEYVEWGMRRGLHVDQFVPQLSFFFNAHNDFFEEIAKYRAARRIWHKAMVERYKSKN
                     PRSWALRFHTQTAGCSLTAQQPYNNVVRTAIQALAAVLGGTQSLHTNSLDEAWALPTE
                     FAATIALRTQQIIAHETGVTNIVDPLGGSYFVETLTNEVERGAWEYIERIDAMGGMVA
                     AIERSYPQREIAEASYRYQMAVDKKEKIIVGVNDFVAKEKPLDILQIDETVAHRQAER
                     LSKLRADRSQAEVDRRLNALRAAAKGKENLMPFIYDAVKSYATLGEICDALRDVFGTY
                     EEVAIT"
     gene            complement(5870..>7270)
                     /locus_tag="VGR03_08080"
     CDS             complement(5870..>7270)
                     /locus_tag="VGR03_08080"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV1994273.1"
                     /translation="QGFQTGTTFFTAWDNTRNSSTSAANLFNPSVTSTIFVGFQQQLL
                     NGFGRSVNTRNIRIAKNNRKIADWAFTQQAITTVTNTITAYWELVFARENVKVQQQAV
                     TVAEKLYNDNKKQLEIGTMAPLDVTRAESELATNRQNLIVAQTLHLQNQQILKNAISK
                     NPLAPNLVNVEIIPTDLPQRPEAIEAPSFEEAAKEAFAKRPELQEEALNLLNSDIDVK
                     ATRNALLPTALLSAQYSSVGLAGNQSKFTTKTVAGAPVVDANGNPVAGDFLPATQFTP
                     NGIMQAGFTDALSSVFHNNFPDYQVSLNVTIPIRNRSAQADNQRAILTQHQLEAQLQL
                     LKNTALLDVRNTYIALTQDRAQVDAASKARELQQQTFNAEQKKYQLGASTVYLVIQTQ
                     RDLINSQGTELRALANLVEAKANYERAVGRTLEVNRVTIADAKSGEAERETLIPGTLH
                     GQVVGTEKLSFNAGQK"
BASE COUNT         1447 a         1902 c         2371 g         1550 t
ORIGIN      
        1 gacttcgccg attttcaaac cgggcgatgc agaagcggat ctttcggcga cgattctcgg
       61 gggcgggaag tccagccggc tgtacaaaaa actcgtttac gaaaaacaga tcgcgcagga
      121 cgtggcggtg aatcagcagt cgctgattct cggttcggtg tttgaagtgc aggcgacggc
      181 aaaagccgga gtgaaacccg aagatcttga gaaagcgatc aacgcggagc tggaggcgtt
      241 tcgcaaagag gggccgacgg ccgcggagct gacgcgagcg cgaaacgtga tcgaatcgag
      301 aatcatcgcg ggtctcgaga cgcttggcgg attcggcggt gttgccgacc gcttgaacag
      361 ctacaaccac tacttgggaa cgccggattt tctggcggcg gacatcgggc ggtacgagag
      421 cgcctcgatg gaatcgattc aagcgttcgc gcagggccag ctcaatggga atcaacgcgc
      481 ggtggtctat ggcctgccgg ggaaacagga tctaggggca gaggtggcga ctccgaaagc
      541 ggaacagaaa gacccatcga agaacaacgg cgagccggta aacgtggacg cggaatggcg
      601 caaggacgcg ccaaaggcgg gcccggccag cgcgttgcat ttgcccgtgc cggaaaaatt
      661 caagctttcg aatggattga ccgtgatgta cagcgagagg ccgggactgc cgctggtggc
      721 ggcgaatctg gttttgcacg ccggaaccgg agtgaaccca gtggatcgcc cggggcttgc
      781 gagcatgacg gcgcggatgc tgcagcaggg aacgacaacg cggtcggcgc tgcagattgc
      841 agatcgcgcc gccgacctgg gcgcgactct gaattcgggc gcgggtgcgg atacgacagg
      901 aatttccacg cgctcactct cccggaattt tccggacgcg ctggaattgc tcgctgacgt
      961 tgcgctccat ccgagctttc cgcaggcgga aattgagcgg gtacgctcgg agcggttgac
     1021 agccattgtg caagaaaaag acgagccatt ttctctagcg acacgcgtgc tgacggcggc
     1081 gctctatgga tcgcgccaca cttacggcta tccggacagc ggcacaacgg aatccattaa
     1141 ggcgatttcg cgcgacgacc tggaacattt ctggaagcaa aattactttc cggacgatgc
     1201 ggcgctggtc gttgcgggaa acatcaagct gacggcgttg aagccgctgc tggaaaaaca
     1261 gttcggcgcg tggaaggccg ggaggcctgc tgcgacggtg atgggttcgc cggagacaac
     1321 ggacgcgaag ctgatacttg tggaccgtcc gggagcgccg cagacgacgc tggtgtgttt
     1381 ctcgatgggg ctggctcgat cgacgccaga ctacgcgccc gtggaggtga tgaacacgga
     1441 tcttggcggg cttttctcca gccgcatcaa catgaacttg cgagagcagc acggctatac
     1501 gtatggggcc ttttccttct ttatgtatca tcgcgccccg gggccgttca tcgccggctc
     1561 cgacgttcga acggacgtga ctgctcccgc gacaacggaa gtgtttaacg aactgcgacg
     1621 aatgcgcgac acgcagatga cgccggcgga actgatcctt tcgaaggact ccatcgcgcg
     1681 gtcgctgccg ggacggttcg agcacggcac ggatgcggcg gcgagcttcg ccgagctttt
     1741 cacttacgat ttgccgctgg actactactc gactctttcg gaccgcatca atgccgtgac
     1801 cgtcgagcaa gcgcaagcca tggcgcagaa atatatcctg ccggaaaaga tgatcgttct
     1861 tgcggtaggc gaccgctcga agatcgaaga agagatgaag aagctgaatt tgggcaaggt
     1921 cgagattcgg gacaccgacg gaaaaatcgt gaagtgagtt tctcggtcca tgacttgaga
     1981 gggcagagag cttgatgacg gcgaatccgc ttttcctcag ccttgaattg gcgcggcgaa
     2041 tcgagttggc ggaagcccaa gcggccgtgg attgcgcgga aacgctggat cgtctgcagc
     2101 cgggaagcgg agcggcggta gaagagattg cgggcggctt tgcgatttat tgcggggcaa
     2161 attcgccaac cacgcaggct gttggcctgg ggcttgcggg cccggtcagc gaggaagaat
     2221 ttgacggcct ggagaccttt taccgaagcc gcggtgaacc tgttcgcgtg gagacgtgcc
     2281 cgctcgcgga agcttccatt atcgagcact ttggcaagcg ctgctatcgc gtcacggaat
     2341 tttccagcgt gatggcactg ccgctggatg acgcgaatcg cgtgcagagc tggcacgggc
     2401 cgcctcccgg catgaccatc gaaaaactgg ggcacgatca gatcgacttg tggacgatga
     2461 cggtggcgca gggattcgca gaagatgcgc cggtggtgcc ggaaattctc gaggtgatga
     2521 aaatgttcgc gttgggcccc agcgcggaat gctatctggc gcggattgac gggaatgtgg
     2581 cgggcggcgc gacgctggcg atccgcaatg gagtggctgg cctgttcgga gcgagtacgc
     2641 tgcctgctta tcggaatcgc ggcgtgcaaa ccgcgttgtt gcacgcgcgc ctctcgcgtg
     2701 cggcggaggc gggatgcgac ttggccgcaa gcctaacgcg gccggggagc acgtcgcagc
     2761 gcaacaccat gcgacaacac ttccaggtcc tgtacacgcg tgtgaaattt gagagcgggt
     2821 tcgagtctgg caatcttgcc cagtgagggc gcagcatgct gcgcccctac aagaaaactt
     2881 agtgcgcgac gcgcttgtgg atgaactcca cgatgtcctg ggtggaggtt ccgggaagaa
     2941 agatttcggc gatgccggat tgtttcaggg cggggatgtc ggcttcgggg atgatgccgc
     3001 cgacgaggac ggtgacgtcg gtcatgcctt tgtcgcggag gagtttcatc aactggggac
     3061 agatggtgtt gtgagcgccg gagagaatcg agagaccgat gacgtcgacg tcttcctgga
     3121 cggcggcgga ggcgatcatt tcgggggttt ggcgcaagcc ggtgtaaacg acctccatgc
     3181 ccgcgtcgcg caaggcacgg gcgattattt ttgcgccgcg gtcatggccg tccaggcccg
     3241 gcttggcaat caagactcga atcttctttt gcatcataag tagatattac tatgcctcgg
     3301 gattcgcaag acggggcttg tcagtttgtc agactgtagc ggcagctcgg gtcggcgagc
     3361 ttgaagtaac tgtgccactt tggttcgacg tgttcgacga acacgatata ctggccgctc
     3421 gcctggccga caatctccag gacatagcgc cccggcaagg cccggaatcc aaagtggcct
     3481 tgactgtcag tggtctccct gcgcacagca gacttccgga tttcatccaa aactttttca
     3541 agagcgaagc agtgttttgt atccgagagg ttgttgtagc cagccaaagt tgagcgagcc
     3601 aaatcgtcgg tgttctcggc ggggaaaacg cgagcgtttg cgtcctttgc ttgctggttt
     3661 tgttgctgca agtcagcgag cgcagcagtt aggagccgct ctcgctcttc cgtcacctca
     3721 tacagcgtga cgcgcgcgcc cgagagcgcc acagcgggtc cattggaacc ggccaaggac
     3781 acgtctccag tgattttcga agtttccagc cagatgatga cgatcaacgc aattacggct
     3841 aatccgacga acaagctaag gtattttttt agaaacaaca cgaccggcac ctcaaatccg
     3901 actaaagttc gttcgcgccc gagctgtgta tcacctgcga aggcacttta gccccgttaa
     3961 gtgatggcaa cttcttcgta cgtgccgaag acatcgcgga gggcgtcgca aatttcgccg
     4021 agggtggcgt aagatttcac ggcatcgtag atgaacggca tgaggttttc tttgcctttg
     4081 gcggcggcgc ggagggcatt gaggcggcgg tcgacctcgg cttgggagcg atcggcacga
     4141 agtttgctca agcgctcggc ttggcgatgc gccaccgtct cgtcaatttg cagaatgtcc
     4201 aggggctttt ccttggctac gaaatcattg acgccgacga tgattttttc tttcttgtcg
     4261 acagccatct ggtaacggta ggaggcttcg gcgatttccc gctgcgggta gctgcgctcg
     4321 atggcggcga ccatgccgcc catggcgtcg attctctcga tgtattccca cgcaccacgc
     4381 tcgacttcgt tggtgagcgt ttcgacgaag taggagccgc cgagcggatc gacgatgttg
     4441 gtaacgccgg tttcgtgagc aatgatctgc tgcgtgcgca gcgcaatcgt cgcggcgaac
     4501 tccgtcggca gagcccaggc ttcgtccagc gaattggtat gcaacgactg agttccaccg
     4561 agcactgcgg cgagggcttg aatcgcggtg cgaacgacgt tgttgtaagg ctgttgcgcc
     4621 gtcaaagagc agcccgcggt ttgcgtatgg aagcgcaggg cccaggagcg cggattcttt
     4681 gatttgtagc gctcgaccat ggctttgtgc cagatgcggc gggcggcgcg gtacttggcg
     4741 atctcctcga agaaatcgtt gtgagcgtta aagaagaagg aaagttgcgg cacgaactga
     4801 tccacgtgga ggccgcggcg catgccccat tccacgtatt ccatgccatc gcgcagagtg
     4861 aaggccagtt cctgaatcgc ggtcgagccg gcttcgcgga tgtgatagcc gctgatcgat
     4921 atcgggttga acttcggtgt gttcttcacg ccgaattcaa acgtgtcgat caccagccgc
     4981 atcgagggtt ccggcggata gatgtattcc ttctgcgcga tgtattcctt caagatgtcg
     5041 ttctggagcg tgcccgaaat tgttttccag tccgcgcctt gcttttcagc aacagccaga
     5101 tacatggccc agatgaccgc ggcgggtgaa ttgatggtca tcgaggtggt gacgttggcg
     5161 aggggaattt tgtcgaagag aacttccata tcggcgagcg aactgatggc cacgccgcac
     5221 ttgccgactt ctccttcaga gagcgcgtga tcggagtcgt agcccatcag tgtgggcaag
     5281 tcgaaggccg tggaaaggcc ggtctggcct tgcgcgagga gatagcggaa gcgctggttg
     5341 gtgtcttcgg ctgccccgaa gcccgcgaac tgccgcatgg tccagagccg gctgcggtgc
     5401 atcgtcgaat gaatgccgcg cgtatacggc ggctcgcccg ggaaaccgag gtcccgctcg
     5461 gggtcccaat ccggcagatc cgccttggtg tacagcctgc gaatcggata cccggaaatg
     5521 gtggtgaatt cggattgtct ttcggagcct ttttccagcg ccggctggag cgtgtttttc
     5581 tcccaggctt gttctttggc ggagggcttt cgatccgctg aggttgattc gagtggaggg
     5641 gcggcctgcg tggtgccttt ggattgttcg ctcatgtgca tcgcccccaa cagggattcg
     5701 aaaaaacgaa tcgcggcata accaagatta atgctatgcg aaagggccgg aaggggcaag
     5761 cacaccgtgg attcggtgca gagaacaggg aactaggcgg aacacggcaa aacaaaaaaa
     5821 cggcccgggc tggagcgccc gggccgttga gaagagtccg ttgcgaaaac tacttttggc
     5881 ctgcgttgaa cgaaagcttc tccgtgccaa ccacttgacc gtgcagtgtg ccagggatca
     5941 gcgtctcgcg ttccgcttcg ccagatttgg cgtcggcgat agtgacacga ttgacttcca
     6001 gcgtgcgccc gacggcgcgc tcgtaattgg ctttggcttc gactagattg gccaaggcgc
     6061 gcaactccgt gccctgggag ttgattaggt cgcgttgggt ctgaatgacg aggtataccg
     6121 tcgatgcgcc gagctgatac tttttctgct ctgcgttgaa cgtctgctgc tgcagttcgc
     6181 gcgccttgct ggcggcatcg acctgggcgc ggtcttgggt gagcgcaatg taggtgttcc
     6241 gcacgtcgag cagcgcggtg tttttcaaca gttgcaactg cgcctcgagt tgatgctgcg
     6301 tcaggatggc gcgctggttg tccgcctggg ctgaacggtt gcggataggg atcgttacgt
     6361 tcaacgacac ttgatagtca gggaaattat tgtgaaacac cgaactcaac gcgtcggtga
     6421 agccggcctg catgatgccg tttggtgtga attgcgtcgc gggaagaaag tcgcccgcaa
     6481 ccggattccc atttgcgtcc acgaccggcg cacctgcgac agtcttagtg gtgaacttcg
     6541 actggttgcc tgcgagtcca accgagctat attgcgcgga gagcaaggcc gtcggcagca
     6601 gagcgttgcg cgttgctttg acgtcgatgt cgctgttcag caggttgagc gcttcttcct
     6661 gcaattccgg cctcttcgca aatgcttcct tagcggcttc ttcaaacgag ggggcttcaa
     6721 tggcctccgg ccgttgcggc aggtccgtcg gaataatctc gacgttgaca agattgggtg
     6781 ctagcgggtt cttgctgatg gcgtttttca gaatctgctg attttgaagg tgcaaggtct
     6841 gcgccacaat caaattctgg cggttcgtgg cgagttcgga ttcagcgcga gtgacgtcga
     6901 gcggcgccat ggtgccgatc tccaactgtt ttttgttgtc gttatagagc ttttcggcga
     6961 cggtcacggc ctgctgctgg acttttacgt tttcccgggc gaagaccagc tcccagtaag
     7021 cggtgatggt gttggtcacg gtagtgatgg cttgttgggt aaaggcccag tccgcgatct
     7081 tgcggttgtt tttggcgatg cggatgtttc gagtgttgac ggagcggccg aatccattga
     7141 gcaactgttg ctggaacccg acaaaaatcg tagatgtcac ggaggggttg aacaagttgg
     7201 cggcggaagt ggaggagttc ctggtgttgt cccatgcagt gaagaaggtg gttccggtct
     7261 ggaagccctg
//
LOCUS       DASYVO010000243         1687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_4246, whole genome shotgun sequence.
ACCESSION   DASYVO010000243 DASYVO010000000
VERSION     DASYVO010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4246"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VGR03_08085"
     CDS             <1..314
                     /locus_tag="VGR03_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994274.1"
                     /translation="TIAGVSAQGTRITHTIPAGQIGNEKPITIVHEHWYSNDLQMVVM
                     SKRSDPRFGETTYKLTNIQRSEPAASLFTVPSDYTVTQGGPGHHGNVIFKQAPPPPPE
                     N"
     gene            complement(602..>1687)
                     /locus_tag="VGR03_08090"
     CDS             complement(602..>1687)
                     /locus_tag="VGR03_08090"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEV1994275.1"
                     /translation="HIDKCLDCRACETACPSGVEYGKLVEHARARIEGDFPRPWLAQV
                     THNFVFRHLLPDPHRIIDAARVLRLYQRSGLQAIARSIGILKLLGLAERERFLPRIDD
                     GFFYSRFGWTYPAAGPRRARVAFFAGCIANVTFTQLNEATLRVLTANGCEVVVPKGQL
                     CCGALAAHAGVRDAARDLARKNLTVFLREDFDAIITNAAGCGSTLKEYDHLFSPDEPE
                     HVQARAFASKTRDVTEFLAALGLVAKLKPLPLRVTYQDSCHLLHGQKVREAPRTLLRA
                     IPKLEFVELPDTEICCGSAGIYNVTQTETSLQLLAEKMRHAQSTRAQTIVTANPGCLL
                     QLRAGATIHKTNQEVLHVVELLDRAQT"
BASE COUNT          357 a          407 c          598 g          325 t
ORIGIN      
        1 agacgatcgc tggcgtgagc gcgcaaggaa cacgaattac tcacaccatt cccgcgggac
       61 aaatcggaaa tgaaaagccg attacgattg ttcacgaaca ttggtattcc aacgatctgc
      121 aaatggtagt catgagcaag cggagcgatc cacgcttcgg cgaaaccacc tacaagctga
      181 caaatatcca gcgctcggag ccggctgcgt cgctcttcac tgtgccgtcg gactacacgg
      241 tgacgcaagg cggacccggc caccacggca atgtgatatt caagcaagcg ccgccacctc
      301 cgccggaaaa ttgattctgc ggattccttc ttggggaccg tgaaacggtc aatgtccttt
      361 gcgaccgtaa gacggccaac acgatcttct ctcctgaaga cggcatgccc ttttcgcagc
      421 agcccccatc gctgcgggaa gggcgctgcc gtttctggaa gcaatgaagt aaggaggcag
      481 ggaggtaagg aagtaaggaa gtaaggaaaa aacaaattcc gcagcgactt ttccgcgggc
      541 acagagcatt tggcgaagag ggtgccgagc ggaagctggt attggatggg agagagttga
      601 ttcatgtttg ggctcgatcc aggagttcga cgacatgaag gacttcctgg ttggttttgt
      661 ggatggtggc gccggcgcgc agttggagga ggcagccggg gttggcggtg acgatggttt
      721 gcgcgcgggt ggattgggcg tggcgcattt tttcggcaag taattgcagg gatgtttctg
      781 tctgggtgac gttgtagatt ccggcggagc cgcagcagat ttccgtgtcg ggcaattcca
      841 caaattctag cttcggaatg gcgcgcagaa gtgtgcgcgg agcttcgcgg actttttgtc
      901 cgtgaaggag atggcaggaa tcctggtagg tgacgcggag tggaagcggc ttcagttttg
      961 caacgagacc gagagcggcg agaaattcgg tgacgtcgcg agtttttgac gcgaatgcgc
     1021 gggcctggac gtgttcgggt tcgtcgggtg aaaaaagatg atcgtattcc ttgagcgtgg
     1081 agccgcagcc ggcggcgttg gtgatgatag cgtcgaagtc ttcgcgtagg aagaccgtaa
     1141 gatttttgcg agcgaggtcg cgcgcggcat cgcgaacgcc tgcatgcgcg gcgagggcgc
     1201 cgcagcaaag ctgtcccttg gggacgacga cttcgcagcc gttggcggtg aggacgcgga
     1261 gggtcgcctc gttgagctgg gtgaaagtga cattcgcgat gcagccggcg aagaacgcga
     1321 cgcgcgcgcg gcgcgggcct gccgcagggt aggtccagcc gaagcggctg tagaagaaac
     1381 cgtcgtcgat gcgcgggaga aagcgttcgc gttccgcgag accgagaagt ttcagaatgc
     1441 cgatgctacg cgcaatggcc tgcaggccgg agcgctggta gaggcgaaga acgcgggcgg
     1501 cgtcgatgat gcgatgcgga tcggggagca ggtgccggaa aacgaaattg tgcgtgactt
     1561 gagcgagcca ggggcgggga aaatcgccct cgatgcgggc gcgggcgtgc tcgacgagtt
     1621 ttccgtattc gacgccggag gggcaggcgg tttcgcaggc gcggcagtcg aggcatttgt
     1681 cgatgtg
//
LOCUS       DASYVO010000244        11796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_274665, whole genome shotgun sequence.
ACCESSION   DASYVO010000244 DASYVO010000000
VERSION     DASYVO010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 11796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11796
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274665"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VGR03_08095"
     CDS             complement(<1..796)
                     /locus_tag="VGR03_08095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEV1994276.1"
                     /translation="MSEMATLAPPMAEQNVPEQTPARFSDALHRYWGYESFRPGQESI
                     VRSIAAGRDACIIMPTGGGKSLCYQLPAVLNPTTAVVISPLIALMHDQVAQLLQMGIS
                     AACLNAATPPEERNHILQHAATGKYQLLYLSPERIALSGTVDWLKHLPISFFAIDEAH
                     CISEWGHDFRPEYRQLSRLRELFPDRPIAAFTASATQRVRHDIIELLCLRDPYKHIAS
                     FRRPNLRYIVRQSNARTQDELLLRSVRQVSDGSTIIYAPTIARVGET"
     gene            1026..2204
                     /locus_tag="VGR03_08100"
     CDS             1026..2204
                     /locus_tag="VGR03_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEV1994277.1"
                     /translation="MPTGPERELEIYALGLGGGKPSVPVPLDMLEQKAKEILPPRAYD
                     YIAGGAGGEDTMRANREAFYRWRIVPRMLRDVSKRDLSVEVLGARLPAPVLLGPVGVQ
                     EIVHDEADVARARAAASLGLPFVLSTMSSRTIEEVAQSAAKVAESPRWFQLYWGKNPE
                     LTASMVQRAERAGYSALVVTLDTHSLGWRERDLHHGYLPFMLGQGLANYFSDPVFRGL
                     LAEAPEENPVAAIQLWGSLFSNSALTWKDIGFLRQHTRVPIILKGILHSNDAARALDA
                     GVDVLIVSNHGGRQVDGGVGALDALPGVVREVNGRVPVLFDSGIRRGADVFKALALGA
                     RAVLLGRLYMWGLAVAGEEGVRDVLLNLVADLDLTMALSGYTCCRELDASALCGSDSI
                     "
     gene            2357..3772
                     /gene="thiC"
                     /locus_tag="VGR03_08105"
     CDS             2357..3772
                     /gene="thiC"
                     /locus_tag="VGR03_08105"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF006763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEV1994278.1"
                     /translation="MGLRDSWIEKREAAVAGANGNRNVSQMHLARQGVISEEMQFVAG
                     REKLEPELIRSELARGRMIIPANINHRNLEPMAIGIASKCKINANIGNSSTSSNIDEE
                     LKKLHHAVHYGADTVMDLSTGGDIPVIRKAIIDASPVPVGTVPVYEAISRVRRPEDLT
                     IGLMLEVIEEQAEQGVDYMTIHAGVLREHVPLVRKRITGIVSRGGSLMAHWMEHHKKQ
                     NFLYENIEDICKIFKKHDVSFSFGDGLRPGCIADASDEAQFAELKVLGELTTKAWEHD
                     VQVMIEGPGHVPLDKIKEQVDKEMEWCHEAPFYTLGPLVIDIAPGYDHITSAIGAAMI
                     GWHGASMLCYVTPKEHLGLPNAEDVKQGVIAYKIAAHAADVARHRPGAQDRDDALSYA
                     RFLFDWNKQFELSLDPPTAKAMHDENLPDDFYKEAKFCSMCGPKFCSMNITQMADAEG
                     GHDQAERKQKFVELLAKVQGA"
     gene            3886..4182
                     /locus_tag="VGR03_08110"
     CDS             3886..4182
                     /locus_tag="VGR03_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994279.1"
                     /translation="MSVHIVIELVKGLAWLGENFPDNPRLGRKLLLGGGFLLCAFFVP
                     ERPEEWADAILIVKVLSEIAGSVCLILGGFIFFRRGVWRRQEKRPPIITSIRWK"
     gene            4189..4878
                     /locus_tag="VGR03_08115"
     CDS             4189..4878
                     /locus_tag="VGR03_08115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1994280.1"
                     /translation="MADTTQSSLLREEFNRWAEAGRGEGMEQDHLPITLPVLEKMRFV
                     PTDNVLDVGCGAGWLSRRLAKLVPEGRVVGMDISDEMIRHARRSSVDFDNLMFVMGEA
                     AQIPWQPHFFSHVISVESSYYWPDPAAGIKDIFRVLGDGGSAWILLNYYRDNPHCHQW
                     GSLLAVPAHLFSAEEWGGLFRDAGFTNVAHERIVDRSPSPEVYTGRWFRDAAHLRAFK
                     AEGALLVRGTK"
     gene            5020..5964
                     /locus_tag="VGR03_08120"
     CDS             5020..5964
                     /locus_tag="VGR03_08120"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEV1994281.1"
                     /translation="MTEAASKLCVLVVDDSPVYRKLVEHALEDEAYAPVFAKNGREAL
                     ELYAKHTPSIVITDWMMPDFSGLELCQHIRGDLQHPYTYIIVLTSISEKDNVVKGLAA
                     GADDYLTKPFDPGELLARIGVGRRTIDLHREIDGKNKLLEEMAHTDPLTALPNRRAIE
                     EWAGRQLRGAARHGYSLWVVHADLDSFKSINDSHGHDAGDQVLKEFAAILKENTRASD
                     ISGRMGGDEFLLVLTHVDEAHIRMTVERLRERFASTKFSFGGESASVTASFGVCGFQG
                     KEPPEFSLLVRQADKALYCAKRAGRNQVKIETPGILVP"
     gene            complement(6106..6486)
                     /locus_tag="VGR03_08125"
     CDS             complement(6106..6486)
                     /locus_tag="VGR03_08125"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEV1994282.1"
                     /translation="MTPKKAPPKSITEYINAAPKEAQKKLREMRACIRTSAPGAKESL
                     KWGMPAFSYRRILVMFAAFKQHIGFYPTPSAVRAFANDLSKFATAKGSIQFPLEKPLP
                     LALIRKITAFRVRESIEVDRKWRT"
     gene            6694..7170
                     /locus_tag="VGR03_08130"
     CDS             6694..7170
                     /locus_tag="VGR03_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994283.1"
                     /translation="MKTEDLKPGYECVFTVTDYCDGPLKGIANYLGHPHFYERIFDEV
                     KDNYSDLFRLSPLDAEIFQLAMKAWNIWRRWEIAFHTGKTNIGTHPALPHEAKRRAEL
                     TSVLDRSLAIDPGKAVARIGQFEVLGRPNLPKGVRRPMQVKWSELLSESLSTADVR"
     gene            7252..8169
                     /locus_tag="VGR03_08135"
     CDS             7252..8169
                     /locus_tag="VGR03_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1994284.1"
                     /translation="MRPVIQVSGVRKTYGPTVAVDEVSFEVNDGEIFGLIGPNGAGKT
                     TTMECIEGLRTPDRGTISVLGLDPFRHVYKLQDRIGVQLQQAQLQKRIKVWEAVDLWA
                     SLYQKKAIDGEHLLEQLGLTNKRDAWFMNLSGGQKQRLFIALALINDPEVVFLDELTT
                     GLDPQSRRAIWDLVRGIRERGKTVFLTTHLMEEAERLCDRVAIIEHGRIIDIDRPERL
                     VDRHCPERTVVLATDNPIAEQRFRAILGVEAVICTDLRFTIRGRGDDLVTEVIHCLSE
                     NRIRVTDFRTILPNLEDVFLKLTGHSIRE"
     gene            8178..8930
                     /locus_tag="VGR03_08140"
     CDS             8178..8930
                     /locus_tag="VGR03_08140"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1994285.1"
                     /translation="MMLRGLWKLTWIEIKIFLREPLGAFGTIGFPVLVFLVLGRVAGR
                     RLAPPSLAASSFIRVGLPVLASLLIAVSAVLSLVTIISIYREGGILKRLRATPLRPQT
                     ILTAHVIVKLILSAATLALMVLAGKRYYPVGVHVPLFSFTIALLISTWSFLSIGFLIA
                     SIVPTARFAQPIGAIILYPMIAVSGLFVPVESLPPVLHAVARVLPLTYAVPLLQGIWN
                     GDAWSAHVGDVAALVVVFVVCTALSTKVFRWE"
     gene            9186..10016
                     /locus_tag="VGR03_08145"
     CDS             9186..10016
                     /locus_tag="VGR03_08145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1994286.1"
                     /translation="MSFLSTGVAVVTGAGSGIGRALAQQLAAAGSTLALADIDEAGLL
                     QTAQTIEKKSALVTTHVVDVADEEGVRSFAEDVTRRHGRVTLLINNAGVSLHGDFEEI
                     SLDDFRWLMNINFWGTVYGVKYFLPALKHEKRAHIVNLSSVFGIIAPAGQCAYAASKF
                     AVRGFTEALRHELEGSNVGVSCVHPGGIRTPIARRSRIGAGATASKREENIERFERLA
                     RTPPEKAAARILRGVERREPRILIGSDAYQIDVVQRLRPATYWKTLAKAFEKLAAKSR
                     "
     gene            10114..10674
                     /locus_tag="VGR03_08150"
     CDS             10114..10674
                     /locus_tag="VGR03_08150"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEV1994287.1"
                     /translation="MGVLDQRFEKNFMITSVDYVFNWARKSAIWPLTFGLACCAIEMI
                     ASTTSRFDMARFGAEVFRPSPRQADLMIVAGTVTLKMAPVLKRVWEQMPDPKWCISMG
                     ACSSVGGPFNAYSVLQGVDKIVPVDVYVVGCPPRPEGLFFALLKLQDKIDQMTLAKRP
                     TEVRLKPQMVEEFKKGVMVAQTPEPR"
     gene            10815..11120
                     /locus_tag="VGR03_08155"
     CDS             10815..11120
                     /locus_tag="VGR03_08155"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEV1994288.1"
                     /translation="MAFARAAKTNEITPGTIREFQVEGKAIALANVGGKFYAINNTCM
                     HRGGPLGQGVMDGNIVTCPWHGWQYDVTTGKISQNPTVGVDCYALEVRGEDIFVDAG"
     gene            11281..11739
                     /locus_tag="VGR03_08160"
     CDS             11281..11739
                     /locus_tag="VGR03_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEV1994289.1"
                     /translation="MAITTAQRHYWIFSADPHHYHWDTLFVKGKEMWHGAGAKPDAIR
                     ALKQVRKGDRVLCYHGTPERSLYSIAEVTRDPYPDPHDHKGKNLVADLRAFEKLPRQV
                     TLADLRSNPALKKMRLVKNVRLIISLLTDLEYQEILRMAGIVATPGIPLP"
BASE COUNT         2440 a         3076 c         3825 g         2455 t
ORIGIN      
        1 cggtctctcc cacgcgggcg atcgtcggag cgtagatgat cgtgctgccg tccgagactt
       61 gcctcacgga gcgaaggagg agttcgtcct gcgtccgggc gttggactgg cggacgatgt
      121 accgcaggtt ggggcgccga aagctggcaa tgtgtttgta ggggtcgcgg aggcaaagca
      181 gctcgatgat gtcgtggcgg acgcgctggg tggcgctggc ggtaaaggcg gcgatggggc
      241 ggtccgggaa gagttcgcgc aagcggctga gctggcgata ctccgggcgg aagtcgtggc
      301 cccactcgga gatgcagtgg gcttcgtcga tggcgaagaa ggagataggg aggtgtttca
      361 accagtccac cgtgccgctc agcgcgatgc gctccggcga caagtagagc agttggtact
      421 tgccggtggc ggcgtgctgc aggatgtggt tgcgctcttc tggaggagtg gcggcgttca
      481 agcaggcggc ggaaatcccc atttgcagga gttgcgcgac ctgatcgtgc atcaaggcaa
      541 tcaacgggga gataacgacc gcagtggtgg ggttgaggac ggcggggagt tgatagcaga
      601 gcgatttgcc gccgccggtg ggcatgatga tgcaagcgtc gcgtcctgcg gcgatgctcc
      661 tgacgatgct ttcctggccg ggacggaagg attcgtagcc ccaatagcgg tgcaatgcat
      721 cggaaaagcg tgcgggcgtt tgctcgggga cgttctgttc ggccatcggc ggtgccaatg
      781 ttgccatctc gctcattcct gcctgcagct gctaccgttc ggcgattcga agggtgtgag
      841 caaagcgcct tgcagtattc gcttttattt cgccatctgt caagtacaaa ttctgttatg
      901 gcgtggaagg gtgttgatcg tcgttcatca gcggggagag atgcaagcgc cgggctaaag
      961 cccggcctct acaaaaccaa gtataattct gccgcgatat gagcgcttcg aaaaaaccac
     1021 aggcgatgcc gacaggtccg gaacgcgagc tggagattta tgcgctcggg ctgggcggcg
     1081 gaaaaccgtc cgtgcctgtg ccgctggaca tgctggagca gaaggcgaag gaaattttgc
     1141 cgccacgggc gtacgactac attgcgggcg gtgcgggcgg cgaagacacg atgcgcgcga
     1201 accgcgaagc gttctaccgg tggcggatcg tgccgcggat gctgcgggat gtttcgaagc
     1261 gagacttgag cgtggaggtg cttggcgcgc ggttgcctgc gccggtgctg ctgggccccg
     1321 tcggcgtcca ggaaattgtg catgacgaag cggacgtggc gcgtgcgcgg gccgcggcgt
     1381 cgctggggct gccgtttgtg ctgagcacga tgtcgagccg gacgatcgag gaggttgcgc
     1441 aatcagcggc gaaagtagca gagtcgccgc ggtggttcca attgtattgg gggaaaaatc
     1501 ccgagctgac ggccagcatg gtgcagcgtg cggaacgcgc gggatattcg gcgttggtcg
     1561 tgacgctgga cacccattcg ctgggctggc gcgaacggga ccttcaccat ggctacttgc
     1621 catttatgct ggggcagggg ctggcgaatt atttcagcga tccggttttc cgcgggctgc
     1681 tggcggaggc gccggaggag aatcccgtgg cggctattca gctgtggggc agcttgtttt
     1741 cgaattcggc gctcacctgg aaggacatcg gatttctgcg gcagcacacg cgtgttccta
     1801 ttatccttaa aggaattttg cattcgaacg atgcggcgcg agcgctggac gcgggcgtgg
     1861 atgtgctgat cgtttcgaat cacggcgggc ggcaggtgga tggcggcgtc ggcgcgctgg
     1921 atgcgctgcc aggtgtggtg cgcgaagtga atgggcgcgt gccggtgctg ttcgacagcg
     1981 gaattcgccg tggggcggat gttttcaagg cgctggcgct cggcgcacgc gcggtgctgc
     2041 ttggccgcct ctacatgtgg ggcttggcgg tggcgggcga agaaggcgtg cgcgacgttc
     2101 tcctgaatct ggttgccgat ctggatttga cgatggcgct cagcgggtac acgtgctgcc
     2161 gcgaactgga tgcttccgcg ctgtgcggga gcgattcgat atagccgcag cgctaaccat
     2221 agctttggtc cctgcagcgc gaagttgccg cgcccatccg caggtgccat aattccactt
     2281 tacctcgcag gcttgtggtt cggggcgttt tttttgcgcg ttgcgaagca gttgtgacgg
     2341 ttactaaggg ggttttatgg gactgcgcga tagctggatt gaaaaacggg aagcggcggt
     2401 tgccggggcg aacggaaaca ggaatgtttc gcagatgcat ctggcgcggc agggcgtgat
     2461 cagcgaggag atgcagtttg ttgcggggcg cgagaagctg gaaccggagc tgattcgcag
     2521 cgaactggcg cgcgggcgca tgatcattcc ggccaacatc aatcaccgta acctagaacc
     2581 gatggcaatt ggcatcgcga gcaaatgcaa gatcaacgcg aatattggga actcctccac
     2641 cagctcgaat attgacgaag agctgaagaa gctgcatcac gcggtgcatt acggcgcgga
     2701 tacggtgatg gacctttcca ccggcgggga catccccgtg attcgcaagg cgattattga
     2761 cgcgtctccg gtgccggtcg gaacggtgcc ggtctatgaa gcgatttcgc gggtgcggcg
     2821 tccggaagat ttgacgatcg ggctgatgct ggaggtgatc gaagagcagg ccgagcaggg
     2881 cgttgattac atgacgattc acgcgggcgt gctgcgcgag cacgtgccgc tggtgcggaa
     2941 gcggattacg gggattgtga gccgcggtgg gtcgctgatg gcgcactgga tggagcacca
     3001 caagaagcag aatttcctgt acgagaacat cgaggacatt tgcaagattt tcaagaagca
     3061 cgacgtgagc ttttcgtttg gcgatggctt gcggccgggg tgcatcgcgg atgccagcga
     3121 cgaagcgcag ttcgcggaac tgaaggtgct gggcgagctg accacgaagg cgtgggagca
     3181 cgacgtccaa gtgatgatcg aggggccggg gcacgtgccg ctggacaaga tcaaggagca
     3241 ggtggacaag gagatggagt ggtgccacga ggcgccgttc tatacgctgg ggccgctggt
     3301 gatcgacatc gcgccggggt acgaccacat caccagcgcg attggcgcgg cgatgatcgg
     3361 gtggcacggc gcgtcgatgc tgtgctacgt cacgccgaag gagcacctgg gcctgccgaa
     3421 tgcggaagac gtgaagcagg gcgtgatcgc ttacaaaatt gcggcgcatg ccgcggacgt
     3481 ggcgcggcac cggccaggag cgcaggaccg cgacgatgcc ttgagctatg cgcggtttct
     3541 ctttgactgg aacaagcagt ttgagctttc gctcgatcca ccaaccgcga aagccatgca
     3601 cgacgaaaac cttcccgacg acttctacaa agaagcgaag ttctgctcga tgtgcgggcc
     3661 gaagttctgc tcgatgaaca taacgcaaat ggcggacgcg gaaggcgggc acgaccaggc
     3721 ggagcgcaaa cagaaattcg tggagttgct ggcaaaggtg cagggcgcct gatttgttga
     3781 ggcctcgaag tttgaagagg ccgagaccgt ccgcagtcga caacactgag ccggcccgca
     3841 agcaaccata ggtctcttat cccatcacac cagtggactt aagtcatgtc tgtacacata
     3901 gtcatcgaac tcgtaaaagg gctcgcgtgg ctcggagaaa acttccccga taatcctcgt
     3961 ctgggacgca aactgctgct cggtggcggg tttttgctct gcgccttctt cgttccggaa
     4021 cgaccggagg agtgggcaga cgcgattctc atcgtgaaag ttctctcgga gattgcgggg
     4081 agtgtttgtc ttatcttggg cggttttatt ttttttcggc gcggggtgtg gaggcggcag
     4141 gaaaagcgcc cacctatcat cacctcgata cggtggaaat gaccgggcat ggctgacaca
     4201 acgcagtcca gtttacttcg cgaggaattc aaccgctggg cggaggcggg gcgcggcgag
     4261 gggatggagc aggatcacct gcccatcacg ctgccggtgc tggagaagat gcggtttgtg
     4321 ccaacggaca acgtcctgga cgtaggctgc ggggcggggt ggctgtcgcg gcggctggcg
     4381 aaactggtgc ccgagggccg tgtggtgggg atggatattt cagacgaaat gatccgtcac
     4441 gcgcggcgaa gcagcgtgga ctttgacaat ttgatgttcg tcatgggaga agctgcgcaa
     4501 attccctggc agccgcactt tttttcgcac gtgatttccg tggagtcttc gtattactgg
     4561 cctgatcccg cggcgggcat aaaagatatt tttcgggttc tcggcgatgg tggctcggcg
     4621 tggatcctgc tcaactacta ccgcgacaat ccacactgcc atcagtgggg aagtctcctc
     4681 gcggtgccgg cgcatctttt ttccgccgag gaatggggcg ggttgttccg tgatgcggga
     4741 ttcacgaacg tcgcgcacga acgcatcgtc gatcgatccc cgtcgccgga agtttatacg
     4801 ggccgttggt tccgcgacgc ggcacatctc cgtgctttca aggcagaagg ggcattgctt
     4861 gtccgcggaa cgaagtagga aggacaggtt actgttttga accaaatgga gcaggtacag
     4921 gttttgcacc agcaagcgct gctaacatta ggctcacgtt atccctgcca tagggccgtc
     4981 taagcattcc agaccggttg ggttgcgttc gagagaggca tgactgaagc tgcgtccaaa
     5041 ttgtgtgtgc tggtggtgga cgattcgcct gtctaccgca agctggtcga acacgctctg
     5101 gaggacgagg cgtatgcgcc tgtttttgcc aagaacgggc gcgaggcgct ggaactgtac
     5161 gcgaagcaca caccgtcaat cgtgatcacc gactggatga tgccggattt ttccggattg
     5221 gagttgtgcc agcacattcg cggagatctt cagcatcctt atacgtacat catcgtgctg
     5281 acgagcatct cggaaaaaga caacgtggta aaaggactgg cggcgggcgc ggacgactac
     5341 ctgaccaagc ccttcgatcc gggcgagttg ctggcgcgca tcggagtggg gcgccggacc
     5401 atcgacctgc accgcgaaat cgacggcaag aacaaattgc tggaagaaat ggcgcatacc
     5461 gatccgctca cggccttgcc caaccggcgc gcgatcgaag aatgggcggg gcgtcaattg
     5521 cgaggggcgg cacgccacgg ctattccctt tgggtggttc acgcggacct ggattctttc
     5581 aagagcatca acgacagcca tggccatgat gccggcgatc aggtgctgaa ggaatttgcc
     5641 gcaattctga aagaaaatac gcgagcgtcg gatatcagcg ggcgaatggg cggggatgaa
     5701 tttctattgg tgctgacgca cgtcgacgaa gcccacatcc ggatgacggt ggaacgcctg
     5761 cgcgagcgct ttgcctcgac gaagttttcc tttggcggcg aaagcgcttc ggtgacggcg
     5821 agtttcggag tctgcggatt ccagggcaaa gagcctccgg aattctccct gttggtgcgg
     5881 caggcggaca aggctctcta ttgcgcgaag cgcgccggcc gcaaccaagt caagatcgaa
     5941 acgcccggaa tcttggtccc gtaaccccgc gcattcgcag atattaagtc agttttcggt
     6001 tatccgactt acacggagtt agtccccacc tctaccccgg atgcaaaggt ctaagctgat
     6061 gttatcccgt taaatgtagg gagtggtttc aggtatcgtt ctccgtcacg ttctccattt
     6121 cctgtctacc tcgatactct ctcgaacgcg gaacgcggtg atcttgcgga tcagagctag
     6181 aggtagcggt ttctccagcg gaaactggat cgacccttta gccgtggcaa atttcgaaag
     6241 atcattcgcg aaggctctca ctgctgacgg cgtgggatag aagccgatgt gctgtttgaa
     6301 cgcggcgaac atgaccagta tcctccgata ggaaaaggcg ggcatccccc acttcaggct
     6361 ttccttggcc ccgggtgcag atgtgcgaat acacgcacgc atttcacgca gtttcttctg
     6421 agcctctttg ggggcggcat tgatgtactc ggtgatgctt ttaggtggcg ctttttttgg
     6481 agtcatgatt ttattttatc gactctccta ctgatactcc aatcgctttt gctagccgcc
     6541 tcccggcagc tgcctatgag actgcctcgg cggaaccgaa gctggaagag tcaagctaca
     6601 cagactccca atgtccggta acgcgactaa tcgggacttt gcccgtgatt gtgctataaa
     6661 aagtccaaag gcaagtgaga cttgacccgg accatgaaga ccgaggacct aaaacccgga
     6721 tacgaatgcg tatttaccgt gacggactac tgcgacggtc ctctcaaagg gatagcgaat
     6781 tatcttgggc atccgcattt ctatgaacgt attttcgacg aagtgaaaga taactactcg
     6841 gatttattcc ggctctcgcc cctcgacgca gaaatctttc agttggcaat gaaagcttgg
     6901 aacatttggc gacggtggga gattgctttc catactggaa agacaaacat tggaacgcat
     6961 ccggccctgc cgcacgaagc caagagacgc gcagaactta cgagcgttct agacagatcc
     7021 ctcgcaattg atccaggaaa ggccgtcgcg agaattggac aatttgaggt actcggtagg
     7081 ccaaatctcc cgaaaggtgt tcgcagacca atgcaggtca aatggagtga acttctgtcc
     7141 gaatcgttat cgacggcaga tgtccggtaa tgcgactaat caggacttaa cctaagtccg
     7201 tgatataaac tttcggtccg gttcagtgaa gctacgattt tgcaaattat catgcgtcca
     7261 gtcattcaag tttccggagt ccgcaagacc tacggcccaa cagtggctgt cgacgaggtg
     7321 tcgttcgagg taaacgacgg tgagatcttt ggtttaatcg ggcccaacgg cgcgggaaag
     7381 accaccacga tggagtgcat cgaaggtctg cgcacgcccg accggggcac catctcggtt
     7441 ctgggtcttg atccctttcg tcacgtctac aagctgcagg accgcatcgg ggttcagctc
     7501 cagcaggcgc agctgcagaa gcggatcaaa gtgtgggagg cggtagacct ctgggcttcg
     7561 ctttaccaga agaaagcgat agacggcgaa cacttactcg aacagctcgg cctcaccaat
     7621 aagcgggacg cctggtttat gaacctctcc ggaggccaga agcagcggct gtttatcgcc
     7681 cttgcgctga tcaacgatcc ggaggtggtg tttctcgacg agctgaccac cgggctggac
     7741 ccgcaatcgc ggcgggcgat ctgggatctg gtgcgcggca tccgcgagcg cggcaagaca
     7801 gtctttttga ccactcatct gatggaggag gccgaacggt tgtgcgaccg ggtagcaatc
     7861 atcgagcacg gacggatcat cgacatcgac aggccggaga ggctcgtcga ccggcactgc
     7921 cctgagcgaa ccgtcgttct tgccacggac aacccgatcg cggaacagcg ctttcgggcg
     7981 atcctgggag ttgaggcggt gatctgcact gacttgcgat tcaccattcg cggtcggggc
     8041 gacgacctag tgaccgaggt gattcattgt ctgtccgaga atcggatccg ggtcacggat
     8101 ttccgcacga ttctccctaa ccttgaggac gtctttctta aactgactgg tcactcgatt
     8161 cgcgagtagg ctcgataatg atgctgcgtg gactctggaa actgacttgg atcgagatca
     8221 agatcttcct gcgcgagccg cttggcgcat tcggcacgat cggttttcct gttttagttt
     8281 tcctggtgtt gggtcgagta gccggccgca gactcgcacc accctcactc gccgccagta
     8341 gtttcatccg cgtgggtcta cccgtgctcg cgtcgcttct gatcgccgtc agcgctgtgc
     8401 tgtctctagt cacgattatc tcgatctacc gcgagggcgg tatcctaaag cgactccgtg
     8461 ccacgccgct gcgcccgcag acgatcctca ccgcccacgt gatcgtcaag ctgatactct
     8521 ccgcggccac gctggccttg atggtcctgg caggaaaacg ctactacccg gtcggcgttc
     8581 atgttccatt gttctcgttc acgattgcgc tgctgatcag cacttggagc ttcttgtcga
     8641 tcggttttct catcgccagc atcgttccca cggcgcgctt tgcgcagccg atcggcgcca
     8701 tcattctgta cccaatgatc gctgtctcgg gactcttcgt gcccgttgaa tcgctgccgc
     8761 cagtgcttca cgcggtggcg cgagtgctgc cgctaaccta cgctgtgcct ctactccagg
     8821 gaatctggaa cggcgacgcg tggtcggccc acgtgggcga cgtagcggca ttggttgtgg
     8881 tgtttgtcgt ttgcacggcg ctctcgacga aggtttttcg ttgggagtag caagattgct
     8941 cggtgcactg gtcagcgcag catgaggcac gttattacca gttaactcag gcgatgcgac
     9001 aaacggacag cgcggaagtt tgggagcgga ctgtcaaggg cacctggacg attaacgcat
     9061 gaatcgaaaa ctgacctagt actcatttct ctcattctgg tgacatagct ggaactatgg
     9121 tatgagtggc cccgggcaga cgtctttcgg cgagccgagc ggccaatccg cgcagaggtt
     9181 ttaaaatgtc atttctgagt accggggtag ccgtggtgac gggggcaggg tccgggatcg
     9241 ggcgcgctct ggcgcagcaa ctggcggccg cgggttcaac gctcgcactc gcagacattg
     9301 atgaagcggg acttctgcaa acggcgcaaa cgatagagaa gaagagcgcg ctggttacca
     9361 cgcacgtggt ggacgttgcc gatgaggaag gcgtgcggtc gttcgcggag gacgtcacca
     9421 ggaggcatgg gcgcgttacg ctgcttatca ataatgcggg agtttcgctc cacggcgatt
     9481 ttgaggaaat atctttggac gattttcgct ggctgatgaa cattaatttc tggggcacgg
     9541 tgtacggcgt gaaatatttt ctgccggcgc taaagcacga aaagcgggcg cacatcgtga
     9601 acctttcgag cgttttcgga ataatcgctc cggcgggaca atgcgcttac gcggcgagca
     9661 aatttgcggt gcgcggattc acggaggcgt tgcgccacga actcgagggc agcaatgtgg
     9721 gcgtttcctg cgttcacccc ggcggaatcc ggacgcccat cgcgcgccgg tcgcggattg
     9781 gcgccggcgc aacggcatcc aagcgcgaag aaaatattga gcgattcgag aggctggcgc
     9841 ggactccccc ggaaaaggcc gcggcgcgaa tcttgcgcgg cgtggaacgc cgcgagcctc
     9901 gtatcctgat cggctcggat gcgtaccaga tcgacgttgt gcagcgcctc cgtcccgcaa
     9961 catattggaa gacccttgct aaggcgtttg aaaaactcgc ggcaaaatcg agatgaggtt
    10021 ggtacacttg ccacgctcca gggcgactca gagagttttt gactccagcg gaaacggtga
    10081 atatactcgg cgtcattaag gaaggaccgg ccaatgggag tactggatca acgcttcgaa
    10141 aagaatttca tgattacgtc ggtggactac gtcttcaact gggcgcggaa gagcgcgatc
    10201 tggccgctga cgtttggact ggcgtgctgc gccattgaga tgatcgcttc gacgacttcg
    10261 cggttcgaca tggcgaggtt cggcgcggaa gtgttccggc cgtcgccgcg ccaggccgac
    10321 ctgatgatcg tggcgggcac ggtgacgctg aagatggcgc ccgtgctgaa gcgcgtgtgg
    10381 gaacagatgc cggacccgaa gtggtgcatc tcgatgggag catgctcgag cgtgggcgga
    10441 ccgttcaatg cgtattcggt tttgcaggga gtcgacaaga tagttccagt agacgtgtac
    10501 gttgtcgggt gcccgccgcg gccggaagga ctcttcttcg cgctcttgaa gctccaggac
    10561 aagattgatc agatgactct ggcaaagcgg ccgacggaag tgcgcctgaa gccgcagatg
    10621 gtggaagagt tcaaaaaggg agtgatggtg gcgcaaacgc cggagccacg gtagagaccg
    10681 gagctagcgg tagaaagttg aaaagggatt aggggttagg gaatagggaa aagaacgaag
    10741 aaagagttta acgcagagga cacagaggac ccggagaagg cagagacgta agggaagagg
    10801 agagagaaga agagatggcg tttgcgcgag cagcgaagac gaatgaaatc acgccgggaa
    10861 cgattcgaga gtttcaggtg gagggcaaag ccattgccct ggccaatgtg ggcggcaagt
    10921 tctatgcgat caacaatacc tgcatgcatc ggggcgggcc gctgggccaa ggggtcatgg
    10981 acgggaacat cgtgacttgc ccgtggcatg gctggcagta cgacgtgacc accggcaaga
    11041 ttagccagaa cccaacggtg ggggtggatt gctacgccct tgaggttcgc ggagaagaca
    11101 ttttcgtgga tgcgggctaa ttccctttaa gatattgaaa acaaaatagt tatattgatt
    11161 ttgccgaaat atgaccacct gggcctagtc tgacctgcta gaccctgttt cccctaaccc
    11221 tcatattttg tttcgctttt tgaatcccag cgtgtatatt gctgccgact cgaagcctcg
    11281 atggcgataa cgactgcgca acgacactac tggatcttca gcgccgaccc acaccactat
    11341 cactgggaca ccctcttcgt aaagggcaag gagatgtggc acggcgccgg ggcgaagcct
    11401 gacgcgattc gcgcgttgaa acaggtgcgc aagggcgacc gcgtattgtg ctatcacgga
    11461 actccggagc gctcgctcta ctcgatcgct gaagtcacgc gagatcccta cccggacccg
    11521 cacgaccaca agggaaaaaa cctggtcgcg gacctgcggg ccttcgaaaa gctgcctcgg
    11581 caggtgacat tggcggacct gcggtcgaat cccgcgctga agaaaatgag attagtgaag
    11641 aatgtgcggt tgatcatttc gctactcacc gacttggagt atcaggaaat cctgcgcatg
    11701 gccgggatcg tcgcgacgcc gggaattccg ctgccgtgac gccacgcgcg cggaccctgg
    11761 ctgttccacg agtgaacctg gagccgtgag cggaac
//
LOCUS       DASYVO010000245         4661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_75742, whole genome shotgun sequence.
ACCESSION   DASYVO010000245 DASYVO010000000
VERSION     DASYVO010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75742"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VGR03_08165"
     CDS             <1..403
                     /locus_tag="VGR03_08165"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEV1994290.1"
                     /translation="RMIELELAIDGDFVCRYRADGLIVASPTGSTAYSLSAGGPIVHP
                     AVESFVITPICPHTLSDRPLVIRDSSSIEMKLSGNTESVFLTLDGQRGIPLQPTDIVR
                     ASRAKELLKLIQPPKKSYFEILRNKLKWGEA"
     gene            complement(442..1809)
                     /gene="lysA"
                     /locus_tag="VGR03_08170"
     CDS             complement(442..1809)
                     /gene="lysA"
                     /locus_tag="VGR03_08170"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV1994291.1"
                     /translation="MDSSPRKLKYVDPASFTPHFAWEKRGARSATGEEVVCERVPLVS
                     VAQKFGTPSYVYSRAAIDDAYMELHKGLGALPHTLCFAVKSNGNLSILNHLAKLGSGF
                     DIVSGGELHHLQRIGVRGDRIVFSGVGKDRHEIREALNYRARGRGGRAGILLFNAESE
                     AELDILVEEAARLAGRGSKPAAAAIRVNPDVQAGGHPHIATGRHEHKFGLDWSEAKRL
                     YLVHRDSKWIRWQGISAHIGSQITTLLPFRRALRRLAGFVGELRRAQIDLRYLDVGGG
                     LGVRYSDQKPPTRTEYARLVSQMVRKLGVHLLLEPGRSIIAPASVLLTRVVYTKTNRG
                     KTFVVVDSAMNDLMRPALYGAIHPITKIVREGKRIDAGRKRVDIVGPVCETGDCFLRD
                     WPLGEVKAGDLLAIWVAGAYGMAQSSNYNARCRPAEVLVEGQRARLIRRRETQNDLLR
                     TNVLG"
     gene            complement(1818..3590)
                     /locus_tag="VGR03_08175"
     CDS             complement(1818..3590)
                     /locus_tag="VGR03_08175"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEV1994292.1"
                     /translation="MNPTNEHTTATALNGGEEVSAEVLSTLAEIGEEVNASLDLDEVL
                     ARTAALIKRHIDYEIFGVLMIEGDGSYLRHRFSIGYPRELADNLRIPLGQGITGSAAA
                     TGHSVRVSDTSKDARYINAIDSVRSELAVPLMLRGKCVGVLDIQSRHLDYFTPDQQNI
                     LTLLASRLSVAIENARLFQKVRTQADTLLLLSEVGRETGAILEVEELLRRAAEQTKRV
                     IDYQILSIMLYDEEQKIFRHRVDVKHGHRVQGKLRATASEGIVGAAATLKEPVLVPDV
                     TVDPRYLMVNPETRSELAIPMMHKGKVIGVLDLESPQLNYFTQDHIQTLSILAANLAV
                     SLENARLYEQLARDEARLERDLQAAKRIQGALLRPVPTEDYGLEMAARYLSAREVCGD
                     LYEFLRYGPQQLGIALGDVSGKGTAAALYGAVVIGIMRSLAPQKLQPAEMLKQMNQLV
                     GERKIEGRFMTACFATWQKGRQKLRVANAGQSQPLLYKDGRCGKIELTGFPLGIFEDV
                     TYDEWSVTLESGNILVFHSDGIAETMNSEGQFFGTTRLMKLIEQHHEASATEIADVIL
                     REVDWFTQSAPLSDDRTLVVAKVR"
     gene            complement(3740..4336)
                     /gene="hpt"
                     /locus_tag="VGR03_08180"
     CDS             complement(3740..4336)
                     /gene="hpt"
                     /locus_tag="VGR03_08180"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV1994293.1"
                     /translation="MPRRKKDEQQGTRTPRERVRVLITGARIQRRIQALAKEIRKDFQ
                     GEPLHLVSVLKGGVFFLTDLARHIAGEVSFDFIAVSSYGQKTHSSGQVRLTRDLDSSI
                     EGKTVIVVEDILDTGMTLQYLLRLFQQRKPKHLRVAVLLDKPGRRITAVHADYVGFTI
                     PDEFVVGYGLDYAERYRNLPYVGVLLLGPGLNKKPAED"
BASE COUNT          950 a         1459 c         1216 g         1036 t
ORIGIN      
        1 cagaatgatc gagcttgagc tcgccattga cggcgatttt gtctgccgct accgcgccga
       61 tggcctgatc gtagccagcc cgaccggttc caccgcctat tctctctccg ccggcggacc
      121 catcgtgcac cccgcggtgg aatcctttgt gatcactccc atctgtccgc acacgctgag
      181 tgaccgccca ctggtaatcc gcgactcatc ttccatcgag atgaaactct cagggaacac
      241 ggagtccgtt tttctgactc tcgacgggca acgcggtatc cccttgcagc caacggatat
      301 cgtccgcgcc tcccgcgcga aagagctgct gaagctaatc cagccgccca agaaatcgta
      361 tttcgagatt ttgcggaaca aattgaaatg gggcgaagcg tagccctttg gctgcgccac
      421 aatctacgaa gcgggacaaa actagccgag aacattggtg cgcagcaaat cgttttgagt
      481 ttcgcgccgg cgaatcaacc tagcgcgctg cccttccacc aggacttccg caggacgaca
      541 tcgcgcattg tagtttgacg attgcgccat tccatacgct ccagcaaccc aaatcgcgag
      601 caggtcgccc gccttcactt ccccgagcgg ccaatcccgc aaaaaacaat caccggtctc
      661 gcaaaccggc ccaacaatgt cgactcgttt gcggccggca tcaattctct tcccttcccg
      721 cacaattttc gtaatggggt ggatcgcgcc gtaaagagct ggccgcatca ggtcattcat
      781 cgccgagtct acgaccacaa acgtcttgcc ccgattcgtt ttcgtataca caacgcgcgt
      841 caagagaacg ctggccggcg cgatgatcga acgcccaggc tccagcaaga ggtgcacgcc
      901 aagtttgcgc accatttgtg agaccagccg cgcgtactcc gttcgcgtcg gcggcttctg
      961 gtcggaatat cgcactccaa gtccgccccc aacatccagg tagcgcagat cgatttgcgc
     1021 gcgccgcaac tctccgacaa acccagccag ccgtcggaga gcgcggcgaa atggaagcag
     1081 tgttgtaatc tgcgagccga tatgggcgct gattccctgc cagcggatcc acttggaatc
     1141 acgatgcaca aggtaaagtc gtttcgcctc agaccaatcc aagccgaact tatgttcgtg
     1201 acgcccggtt gcaatgtgcg ggtggccgcc ggcttgcaca tcgggattca cccgaatcgc
     1261 cgccgcagct ggcttgctcc ctcgtcccgc cagtcgtgcc gcctcctcca ccaaaatgtc
     1321 gagttccgct tcggattcag cgttgaatag cagaatgcca gcgcgtccgc ctcgcccgcg
     1381 ggcccgatag ttcagggctt cacgaatttc gtgccggtct ttgccgactc cggagaaaac
     1441 gatgcggtcg ccgcgcacgc cgatgcgctg caagtggtgg agctctccac cggatacaat
     1501 gtcaaaaccg cttcccagct tggcaagatg atttaggatg gacagatttc cgttggactt
     1561 tacggcaaaa cacaaagtgt gcggcagcgc gccaagcccc ttgtgcagtt ccatgtacgc
     1621 atcatcaatc gcagcgcggc tataaacgta gcttggcgta ccaaacttct gcgcgacgga
     1681 gaccagcggc actctttcgc acaccacttc ttcgccggtc gcactccgcg cccccctctt
     1741 ctcccacgcg aaatgtggcg tgaaagaagc gggatccaca tatttaagct tgcgtggaga
     1801 ggagtccatc gaatggctca tcggaccttt gcgaccacca gggtccggtc atcggaaagc
     1861 ggcgcgctct gagtaaacca atccacctcg cgcagaatca cgtcggcaat ttccgttgcg
     1921 gacgcttcat ggtgctgttc gatcagcttc ataagccgtg tcgtaccgaa gaactggcct
     1981 tcgctgttca tggtctccgc gattccgtcc gagtgaaaca caagaatgtt tccggactcg
     2041 agcgtcaccg accattcgtc ataggtaaca tcctcgaaaa tgcccagcgg gaagccggtc
     2101 aattcgatct tgccgcaccg tccatctttg tacagcagcg gctgcgactg ccccgcgttt
     2161 gccactcgca gcttctgccg gcctttttgc caagtggcaa agcacgccgt catgaatcgc
     2221 ccctcgattt tccgttcgcc caccagttgg ttcatttgct tcaacatttc agcaggttgc
     2281 agcttctgcg gtgccagact ccgcatgatt ccgatgacca cggctccata gagcgccgca
     2341 gccgttccct tgccgctcac gtctcccagc gcaatcccca gttgctgcgg cccgtagcgc
     2401 agaaattcat agaggtcgcc gcacacttcc cgcgcggaaa gataccgggc ggccatctcc
     2461 aatccgtagt cttccgtcgg aaccggccgc agcagcgcgc cttgaattct tttcgccgcc
     2521 tgcaaatccc gctccagccg cgcctcgtcg cgcgcgagtt gctcgtagag ccgcgcattc
     2581 tccagcgaaa cagcaagatt cgcggccagg atcgaaagcg tctgtatgtg atcctgggtg
     2641 aaataattca gttgcgggct ttccaaatcc agcacaccaa tcactttccc tttatgcatc
     2701 atggggatag ccagttccga acgcgtctca gggttcacca tcaggtagcg cggatcgacc
     2761 gtcacgtccg gaacaagaac gggttccttc aacgtggctg ccgcgccaac gatgccttca
     2821 ctcgcggtcg ctcgcagttt tccctgcacg cgatggccat gcttcacgtc cacccggtgc
     2881 cggaaaatct tttgctcttc gtcataaagc atgatgctca gtatctggta atcaatcact
     2941 cgcttggtct gttccgctgc gcgccgaaga agctcttcca cctccaggat cgcgccggtc
     3001 tcgcggccga cttcgcttaa tagtaagagc gtgtccgcct gcgtccgcac cttttgaaac
     3061 agccgcgcgt tttcaatggc cacggatagc cggctggcca gcagcgtcaa tatgttctgc
     3121 tgatcagggg taaaataatc gaggtggcgg ctctgaatat ccagcacgcc gacgcacttt
     3181 ccccgaagca tcaacggcac ggccagttcg gagcgcacac tatcgatggc attgatgtac
     3241 cgcgcgtcct ttgaagtatc gctgacgcgc acggagtggc ccgtggccgc cgcgcttccg
     3301 gtaattccct gccccagggg aattcgcaaa ttgtccgcta actcgcgcgg atagccgatg
     3361 gagaatcggt gccggaggta acttccatcc ccttcgatca tcagcacgcc aaagatttca
     3421 tagtcgatgt gccgtttgat caaagccgcc gttcgcgcca gaacctcgtc cagatccagc
     3481 gacgcgttca cttcctcgcc gatttccgcc agcgtggaaa ggacttcggc ggaaacctcc
     3541 tcgccaccat tcaaggccgt ggctgtcgta tgttcgttcg tgggattcat gaagtctgtc
     3601 tattatagca ggcagcgaag ggcgcgccct gccgtggcgc gcatcgaacc gaattctcac
     3661 tctctttcac atctcgagtt gattcgtacg tgctggcgaa tcggatgcag ccctcaggga
     3721 tggttggttc agcgcgattt taatcttccg cgggtttttt gttcaaaccg ggcccaagta
     3781 ataggacgcc aacgtagggc aggtttctgt aacgctcggc gtagtccagt ccataaccga
     3841 ccacaaattc gtcgggaatc gtaaatccca cgtagtcggc gtgtacggca gtgatgcgcc
     3901 gtcctggctt gtccaaaagc accgccacgc gcagatgctt cggcttgcgc tgctggaaca
     3961 gccgcagcag gtactgcaac gtcattcccg tatccagaat gtcttcgaca acgatgaccg
     4021 ttttcccctc gatgctggag tccaggtccc gcgtcaaccg gacttgcccc gacgagtgcg
     4081 tcttttgccc gtagctcgat acggcgatga agtcgaatga aacttccccc gcaatatgtc
     4141 ttgccaggtc ggtcaggaaa aaaaccccgc ccttcaagac gctgaccaga tgcagcggtt
     4201 cgccctgaaa gtcctttcgg atttccttag ccagcgcttg aatgcgtctt tgaatccggg
     4261 cgcccgtaat caagactctc actcgctcgc gcggagtcct ggtaccttgc tgctcgtctt
     4321 ttttgcgtcg cggcatccta ttcctcaaac gtggcaccga gactctaacg cacgcgcgtg
     4381 ctgcacggca agacacactt gtaacgcgcc attcttcgac cgtttatacg gcctcaatct
     4441 gtgctgctta gtctcaggaa agcgattgag gtggcgttta gattgtgttt ttggtgctgt
     4501 ggtgcattga aagtattgct tcaggcgcgc cgaagttttc cacagggttt tacttcaata
     4561 catataattc ctgtgataga agataggtca taaatttgga tgttggttgc tgtcgagagt
     4621 cggcgtttgc ggccactgcg atccctcccc gaaatggggg g
//
LOCUS       DASYVO010000246        17895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_4254, whole genome shotgun sequence.
ACCESSION   DASYVO010000246 DASYVO010000000
VERSION     DASYVO010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 17895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17895
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4254"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..2046
                     /locus_tag="VGR03_08185"
     CDS             217..2046
                     /locus_tag="VGR03_08185"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEV1994294.1"
                     /translation="MKIQDFNERVKDSILIADGAMGSMLHETVGPQRCFDELNSTEPE
                     AVFRVHQAYIEAGAHFIETNTFGANRFKLAPLGLGEEVQRLNSRGVKIAREARESASR
                     EVLIAGSIGPLGLGVQSRHPATEEIQDIFHEQALALEERGVDFYILETFSYIEELLLA
                     IDAIRSFSGLPIVAQLTYSEEGTTYGDVRPSDAAAQLKNKNVQVIGANCTLGPQALLP
                     ILQELAAIDGLRVSGMPNAGFPKREGDRIVYPKSSPEYFALFAREAAALGVRILGGCC
                     GTTPAHIRAMAEAVKSLRPSKTHPVAQSVETVLRPAPVAHRDPESKFWKKLQARQFAV
                     CVEIDPPKGLSLERIFEQVDKVMASKKVDAIDINSGAMARVGMDALIVAGALEARGVE
                     TVPHLTTRDQNIIGLQAALLGAWTVGGVRNILAITGDPPSVGDYPETSGVYEVDSVGL
                     VKVLNRLNQGTDWAGKTLGGATNFTIGVAVNPVADDLDNEIERFHAKVEAGAHFAMTQ
                     PLFDPKHWHAFLKKLGGKPAIPILIGVWPLNSYKQALRLNNEVPGIVIPEPLLKSMEA
                     AGAAARDRGFEVARDMLAWARTELAGAYLIPPFKRYEEILEIL"
     gene            complement(2085..2573)
                     /locus_tag="VGR03_08190"
     CDS             complement(2085..2573)
                     /locus_tag="VGR03_08190"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEV1994295.1"
                     /translation="MEEGFFKRRLVRPILDLLRQGVTPEKIALSVALGAALGVFPAFG
                     CTTLFCAIIAIVLRLNLPAIQIVNYFMYPIQIALLVPFFRLGEKLFRAPRFPISVPQI
                     YGLFHAGAWIAIKLLWTTIWHAMVVWGVIAPVFVGLVYAILMPLLRRALRVRTLQSNI
                     PV"
     gene            complement(2590..3084)
                     /locus_tag="VGR03_08195"
     CDS             complement(2590..3084)
                     /locus_tag="VGR03_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1994296.1"
                     /translation="MRKTRQWVLVAVALATGAFLFVQTQVFAKPADERKADRQAISTV
                     LDAQQAAWNGGDVDAFLVGYLRSPELTFSGSSGVARGWDGVLVRYKKNYPDRAAMGQL
                     DFSELEFRFLGPDAALVLGRWHLKREKDEVGGVFTLVWQRFPDGWKIIHDHTSAVAAA
                     NQNP"
     gene            3297..3689
                     /locus_tag="VGR03_08200"
     CDS             3297..3689
                     /locus_tag="VGR03_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994297.1"
                     /translation="MLLAISIVALSQFALYYWRAVLAGVAAQPVSDRVLVAAQVENGR
                     LTPQHFQTLAGLHDLTPDLHPNRSGLGLVRVYYRVIEGLDAFFGKRIPTFAVWSERER
                     VICARYAAVQVDRRLQANLDLAASLRSC"
     gene            3925..4350
                     /locus_tag="VGR03_08205"
     CDS             3925..4350
                     /locus_tag="VGR03_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994298.1"
                     /translation="MTPIESTLRSVRVLHLIFLLTVALFIINLRMLQPAERGISFLTV
                     SVFVLLCLSDIAIAISFRARMITPSIERLRVNPQDAAALGRWRSGVITSFTFAETVVL
                     LGFMLKILGAGWNVAGVFFVVGILLLLAWAPKLDMSRGN"
     gene            complement(4555..5550)
                     /locus_tag="VGR03_08210"
     CDS             complement(4555..5550)
                     /locus_tag="VGR03_08210"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEV1994299.1"
                     /translation="MSHRIAAKITGVAGFVPPKVMTNADLEKIVETNDEWIRTRTGIR
                     ERHVVEKGMATSHMATEAARAVLAQTKTDPAEIELIVLASVTPDMFFPATACLVQQRI
                     GAKKAWGFDLSAACSGFAYALTVGGQFVGAGTHKKVLVIGSDTMTSVLDYKDRATCVL
                     FGDGAGAVLLEPAGTNEGILDFEHDVDGTGGAFLYMPGGGSLHPSSQETVEKRMHYVH
                     QEGSQVFKYAVRRMADVAVHLLEKNGFTNDDLKLLVPHQANLRIIRATQERLGMDDSK
                     VMVNIDRYGNTTAGTIPLGLRDAVEEGRLHKGDLVLIVTVGAGYTTGGVLLRWAY"
     gene            complement(5640..8006)
                     /locus_tag="VGR03_08215"
     CDS             complement(5640..8006)
                     /locus_tag="VGR03_08215"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEV1994300.1"
                     /translation="MGPVIRNSIIGIIFVFAAATRVSAQATVAPPPNEAKIPLSKIPR
                     VHRAPKLEDFLENHPREAELTVTDFRQNTPGDGTPATESTTVYLSYDDKNLYAAFICH
                     DEAGEVRAHLSKREATDQDDGVGVLLDTFRDFHRAYFFYSNPLGVQTDAIYTEGQGYD
                     FSFDTLWDNAGRVTNDGYIVFFSIPFKSLRFSSEPEQTWGVALYRVILRKSEYDYWPY
                     VTQRVQGLTQQFAPVGGLENVSPGRNIQLIPYGLLANDHFLNQPSGGPPGFVDKFEHR
                     AGLDAKFVAKDSLTFDVTLNPDFSQVESDDPQVTLNQRFAVFFPEKRPFFIENAGFFM
                     TPINLFFSRQIADPQFGTRVTGKLGKWTLGALVIDDRQPGQNSTSSAYNTRAADGVVR
                     VTREFGNQSYIGAFFSTRDFADTSNRVASLDARLKFFKNWVVDVQGVHSWTRQDLKAG
                     VDCLSFADFAKGMGSQQGNALWADASYSGRHFSFSTNYNDYAPNFCTEMGFVNRIDIR
                     QNNAFGGYFWRPDKNKIIDFGPTITETVDWNHAGILQDWGVALGFQVDLTKQTTISVS
                     RGEAYELFDTIGFRKHSTSFLVSTQPYKWISFSTRYTQGIGENFFPAQKLVPFQPFLG
                     NTRRVNFGLTLRPSARFRFDEALIYYRLGTRAGWVTPPFAPGQSVFNNYLNRAKLNYQ
                     FTKELSLRLILDYNATIANTNLFDLQRSLGNSDDVNGPFAPTKQFTTDVLLTYLLHPG
                     TAVYLGYNNGYSDLTLHPGASPFVTAQGAPNNPTSRLFFIKLSYLLRF"
     gene            complement(8121..8534)
                     /locus_tag="VGR03_08220"
     CDS             complement(8121..8534)
                     /locus_tag="VGR03_08220"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEV1994301.1"
                     /translation="MQTGSVKWIGEQKFVATSPSGHAMTIDSDRASNKAPGPMELVLL
                     ALGACTATDVVIILEKKRQKLESLEVICSGERAAEPPTVWTKLEMLYRLRGHLDDAAV
                     NQAIQLSEDKYCSVAAMLRKTATLSWRYEILPPAS"
     gene            8676..9164
                     /locus_tag="VGR03_08225"
     CDS             8676..9164
                     /locus_tag="VGR03_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994302.1"
                     /translation="MQIQVSKVKRKLAMAALGLAALLWSSTIGAGAQQGVVAADGKDQ
                     PYVIEYYYKAKWGHAEEFLALFKKNHYPVLKKEMELGRMLKVSMTVPRYHTTEDGRWD
                     YRVTIVFKNAAIANDNFDSSAMIKQLYPDQETYKREEQRRFEILEAHWDLPIKDVDLE
                     AR"
     gene            complement(9190..12687)
                     /locus_tag="VGR03_08230"
     CDS             complement(9190..12687)
                     /locus_tag="VGR03_08230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEV1994303.1"
                     /translation="MCLREWQCAVRDAGGESKVFLMHQSFISLALLIATTLVAGFFAY
                     IHSIKRQTYLQLWTAAWAVFGLHYVGPTLSPSTAASPLQGALNHWLFALSGVLFFLGA
                     QLYSQRKAWKIPGLVVGVLLGFWAAGNATNAISVSAIFPASLLYIAVAVVFWQESRRQ
                     ETLADRLLGVSFASWGVLWIALDTLNATPELEGAGMSVVAAAPCAFVAMLMIMALYEE
                     EKRRIERNMLALSNLNLATSSFVGGEIQRMLSQALDRVLGVVRLPAGALFLHHGDPQG
                     PTSVVAVGLSDEFCRVAQDEGLDDYLVNLVARLGGLLGFRDLRDDSLSALEKEKSIRR
                     FRELALAQGLRSVVAISLQAKEQAFGVLLLGTPDSRRFTPAELRLLLALGHQIGMAVE
                     NSYLIQQTSRRSEELHVLNEIGRALSSTLNKEDLTRKVWEELRRLFDVENFYIAELDP
                     LRDEIQFDLEIVDGARLPKRARPAGNHITEYIIRTRQPVLIRDNYVGEMKKLGVEPLR
                     SKGCFCGVPLVAYDHAIGAMAVYSDHERVFDEGHLELLRVLASEASIAIENARLFSEE
                     RTKARHLSLLNTISRNAIATLNPDEMLAKITEQLEAGLIYDHIGIGVLDYATREVVIQ
                     AEAGKRRGTLGQRISLGAGLIGHVARNGHMAVYRAASPADGALKPLLPDSVAAIALPV
                     FYAEQLHGILYIESSVPVDFSEEEVLLLRTLADLIAGALHNALSFQKAQEQAITDGLT
                     GVKTHRFFMEALSAEWKRSTRAGRAFALVLMDLDRFKFVNDFYGHLEGDLVLQRVGQI
                     LETNCRRSDVVARYGGDEFVILMPETNMEHARQLATKLRGWVSADPLLREKNISASFG
                     IACYPLHGSSPQELIQVADASMYLSKHQGGNAVSTADHFDPNEAKKWKRDVLEAYLGV
                     TLKRLFSTGPEAFEEIYQRLRQFTESLAATEPNNGNPATTPAQGPQALPQAVLDTVTS
                     LAFAIDAKDHYTQGHSQKVSAYAALIAEAIGMKDAEVEEIRLGAVLHDIGKVGIPGSI
                     LNKNGPLNPEEWETMKSHVKFGAKILDPLTPLARIREMVLHHHEFFDGSGYPEALTGE
                     TIPLGARIIAVADAYDTITSDRTYKKARSATDALAELERCANAQFDGTIVKLFVRTMR
                     ALPNPIIEVAATTGPVRGN"
     gene            12994..14493
                     /gene="gltX"
                     /locus_tag="VGR03_08235"
     CDS             12994..14493
                     /gene="gltX"
                     /locus_tag="VGR03_08235"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV1994304.1"
                     /translation="MSDPIHKTSSVRVRFAPSPTGYLHVGGARTALFNWLFARRQGGT
                     MILRVEDTDAERNKPELVQGILDGLQWLGVNWDEGPFYQSQRTELYRAAAKKCLANGA
                     AFLCYCPTEKYAGGDHAEEGSEAAQLRRASRCSCRGGGKPSNPDVKPAVRFKVPLGET
                     TKFLDAVFGEREFSNDEIEDFVLLRSGKSDEEFGQSMYQLGVVVDDIDMRITHVIRGA
                     DHISNTPKQVLLYRALGAEPPVFAHVPLILGADKSRLSKRHGATDVNMYRTEGFLPEA
                     FRNFLALLGWSPGGDEEFLRTKDLLEKFSLAGVNRTNAVFDRPKLEWFNTQYLQKLPI
                     EELLPEVENELKRSGLWKPEWAAGADPGPDGRDHAWFSKTVDLLRPRIRLLPDFSSWS
                     HAFFSDEFTLDAAAKEKFWKDPKVPELLAKLADALEALAEWNHDACEHASRAVAETGG
                     VKAGLLINATRVAIVGQVVAPPLFDTMVVLGHQRVVRRLRHTVAGFSKA"
     gene            complement(14570..15118)
                     /locus_tag="VGR03_08240"
     CDS             complement(14570..15118)
                     /locus_tag="VGR03_08240"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEV1994305.1"
                     /translation="MKSAQTFFFITVLALSVVLSPAAPSQIPSGRDVVKPKIYVSLDP
                     AGRGSSFQIAVVMKIRPGFHVNAREKSEDYLIATDLKAALPAGFIGGEVSYPKGKLEK
                     FSFSKTPLNVYQDTVILRMPVTALESAPLGEQHIPLKLRYQACSNELCLPPVTLTLDA
                     TLNVAAAASDAKPAHSEIFGKQ"
     gene            complement(15115..15447)
                     /gene="cyaY"
                     /locus_tag="VGR03_08245"
     CDS             complement(15115..15447)
                     /gene="cyaY"
                     /locus_tag="VGR03_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEV1994306.1"
                     /translation="MLDEKDFQRKADAAFEDLKRRMLAAGDEHGFDVEGESGKLEVLF
                     EEPEEVKFVISPNTPVREIWISALSTSFKLGWNESRNAFVHEKTGEDLHAVMSRVISQ
                     QLGAQVTL"
     gene            15887..17593
                     /locus_tag="VGR03_08250"
     CDS             15887..17593
                     /locus_tag="VGR03_08250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1994307.1"
                     /translation="MKRLAHWQLAAVAGFLILYIIVSLVAPRGFALTAFSDITGTGLW
                     VIAIAVMLWAAFSNQGRTRWFWILLAAGAAMVCSNLAAWLYYEVIAGRSPPDPFWADI
                     PLFLQPVPMMTAAAMRPGSRQQEQKFHLSTLNFLILLLWWIYIYMFLVYPNEYILPNK
                     ISFNSYYYTLFVLEFAVLQAVLGGMALVAKGAWRKIYWHLFFAGSLYLLAYQWLNAAL
                     GRDEYYSGSIYDVPNYAAICWFIVIALRARNVPSEPLAGGERDRWADVPGFLAVLAVL
                     SLPVIGLCELFLDPQASQLRPFRVLVTLIGILLVGVCVFLRQILLAREMARLLLDSKQ
                     NLERLQRAQSQLVQKERLAGVGQLVAGVAHELNNPLTAVMGYSDLLIDQAAEGTSRPK
                     LEKLRAEAQRMKRIITNLLTFAQPQHEGRRLLDIAVVVRESLMLLEYQLRNCGISVEM
                     NFAPNLPRIALNEGQFKQVFVNLFSNSTQALEQSQEKKILVEGYLEGEKVIVRFTDSG
                     PGFNDVSRAFDPFYTTRPVGQGTGLGLSICYGTVREHNGNIYAQNLQPNGAAVTIELP
                     LG"
BASE COUNT         3697 a         5192 c         5228 g         3778 t
ORIGIN      
        1 agtaaacctc tgccgcaggt gcccaggacc gcgcccgtcg agcgcattac ccacattctc
       61 agccatgaga atcccgcggt cttcgtcgag atgggcaacg ggcacttcga aatcctcacc
      121 aagtacgacc ttatgagcac cgtcgccagc ctcatggaac aaaaacgctg agcgcatttc
      181 ccgtgtcgaa ttttcatcag acaggaaaca cgaaccgtga agattcaaga ttttaatgaa
      241 cgggtgaaag actcgatact catcgcggat ggcgcgatgg gctcgatgct ccacgaaacc
      301 gtgggtccgc agcgctgctt cgatgaactg aattctaccg agccggaagc ggttttccgt
      361 gtccaccagg cttacatcga agcgggcgcg cacttcatcg aaaccaacac ctttggagcg
      421 aatcgtttta agctcgcgcc cctcggcctc ggcgaagaag tccagcgcct caacagccgc
      481 ggcgtgaaaa tcgctcgcga ggcgcgcgaa tcggcctccc gcgaagtctt gattgccggc
      541 tccatcgggc cgctgggcct cggcgtgcag tcgcgccatc ccgctacgga agagatccag
      601 gacatttttc acgaacaggc gctcgcgctc gaagagcgcg gcgtggactt ctacatcctc
      661 gaaacttttt cttatatcga agaattgctc ctggccatcg acgccattcg ctcgttctcc
      721 ggcttgccca tcgtcgcgca gcttacgtac tccgaagagg gcacgaccta cggcgacgtc
      781 cgcccctccg acgccgcggc gcaattgaag aacaaaaacg tccaggtgat cggcgcgaat
      841 tgcacgcttg gcccgcaagc gctcctgcca attctgcagg agcttgccgc cattgacggc
      901 cttcgcgtca gcggcatgcc caacgccggt tttcccaagc gcgaaggcga ccgcatcgtt
      961 tacccgaaat cttctccgga atattttgcg ctcttcgcgc gtgaagcggc cgcgctcggc
     1021 gtgcgcattc tcggcggttg ttgcggcacg acgccggccc acattcgcgc catggctgaa
     1081 gcagtgaaat cgcttcgccc ctcaaaaacc catcccgtcg cgcagtccgt cgagacagtc
     1141 ttaagacccg cgccggttgc ccatcgcgat ccggaaagta aattctggaa aaagcttcaa
     1201 gccaggcagt ttgctgtgtg tgtcgagatc gatccgccaa agggcctttc gcttgagcgc
     1261 atcttcgagc aggtcgacaa ggtcatggcg tcgaaaaaag tggatgccat cgacatcaat
     1321 agcggcgcca tggcccgcgt cggcatggac gccctgattg tggccggtgc gctggaagcc
     1381 cgcggcgtcg aaaccgttcc tcatctcacg acccgcgatc agaacatcat cggcctgcaa
     1441 gctgcgcttc tcggcgcgtg gaccgtcggc ggagtccgca acatccttgc catcacgggt
     1501 gatccgccgt ctgtcggtga ttacccggaa accagcggag tctacgaagt cgattccgtc
     1561 ggcctcgtca aagtgctcaa ccgcttgaac cagggcaccg actgggccgg aaaaactctc
     1621 ggtggcgcga cgaattttac gatcggcgtc gctgtgaatc ccgtcgccga cgatctcgac
     1681 aacgaaatcg agcgcttcca cgccaaggtc gaagccggcg cgcatttcgc catgacccag
     1741 ccgctcttcg accccaaaca ctggcatgca ttcctgaaaa aactcggcgg caagcccgcg
     1801 atccccatat taatcggcgt ctggccgctg aatagttaca agcaggcgct gcgcttgaac
     1861 aacgaagtgc cagggatcgt tatccccgag cccttgttga aatccatgga agccgcaggt
     1921 gccgccgcgc gcgatcgcgg cttcgaagtc gcccgcgaca tgcttgcctg ggcccgcacc
     1981 gagcttgccg gcgcgtactt gattccgccc ttcaagcgct atgaagaaat cctggaaata
     2041 ttgtagaacc ccatccgcgt cgggaatttt gtagtggccg gtcttcagac cggtatgttc
     2101 gactgtagcg tgcgcacccg aagcgcgcgg cgcagcagcg gcatcagaat cgcatagacc
     2161 aatccaacaa agacgggcgc gatcactccc cacacgacca tcgcgtgcca aatggttgtc
     2221 cacagcagct tgatcgcgat ccacgcaccg gcgtgaaaca agccgtaaat ctgcggcacg
     2281 gaaatcggaa aacgcggcgc acgaaaaagt ttctcgccca accggaaaaa cggcacaaga
     2341 agtgcaattt ggatcggata catgaaatag ttcacgatct ggatggctgg caggttcagc
     2401 cgcagaacaa tcgcgatgat cgcgcagaac aacgtcgtgc atccgaacgc aggaaacaca
     2461 cccaaggccg cgccgagggc cacgctcaga gcgattttct cgggtgtcac tccttgccgt
     2521 agcagatcaa gaatcggccg gaccagccgt cgtttgaaaa atccttcttc catgactagt
     2581 ccagtttagc taaggatttt gattcgcagc ggctaccgcg ctcgtatgat cgtgaatgat
     2641 cttccatccg tccggaaagc gctgccacac cagtgtaaac actcctccga cttcgtcttt
     2701 ctcgcgcttc aagtgccacc gccccaggac gagcgccgcg tccggcccca aaaaccgaaa
     2761 ctccaactcc gaaaaatcca gctgccccat cgcggccctg tcgggataat tcttcttgta
     2821 tcgcaccagc acgccgtccc agccacgcgc tacgccgctg ctcccggaaa acgtcaactc
     2881 cggcgaacgc aaatatccca ccagaaacgc gtccacgtcg ccgccgttcc acgcggcttg
     2941 ctgcgcgtcc agaacagtgc tgattgcttg ccggtcggcc ttgcgctcat cggcgggttt
     3001 ggcgaacacc tgtgtttgta caaagaggaa agcgcccgtt gcaagtgcca cagccactaa
     3061 tacccactga cgagtcttcc gcatggccgg tctccttggc aaacccttgt gcgcagggag
     3121 gataggcgag gcgccgctcc gcggcaagcc ttctctcgag gtgccatttc ctcgcgtaag
     3181 ccaaatgatt ccagtgggtt acaccgaagg ttccagtcca gtggtacttc gggactattg
     3241 gtagcccacg gtccccctgg cagaattcct ttagaggctg agggatgttt agcgcgatgc
     3301 tacttgcgat ctccattgtg gctctgtcac agttcgccct ctattattgg cgggcggtct
     3361 tggcgggagt tgcagcccag ccggtctctg accgggtgct ggttgctgcc caagttgaga
     3421 atgggcgcct cacaccacag cacttccaga ccttggccgg cctgcatgac ttgactcccg
     3481 atctccatcc aaatcgcagc ggattgggct tggttcgcgt ctattatcgg gttatcgagg
     3541 gtcttgacgc cttcttcggc aagaggattc ccacttttgc cgtctggagc gagcgcgaac
     3601 gcgtcatctg cgcacgctac gccgccgtcc aggtcgaccg ccgccttcag gccaacctcg
     3661 acctcgcggc ctcactccgc tcctgctaat agttcctccc cgtcccgatg gaacctgcgg
     3721 tgcgcgccag atccagttcg ctctgaagct cattttctga gatgcatctc ggaacatccc
     3781 aggcgcccgt ggcccgctgg ctgcgggcgc ctttttttta tgcttttatc ccatttctgt
     3841 ccttatcgct tcgttctatc tgttgcgcgg gcagcagaat cagtagagaa tgccatcgct
     3901 ttccgatgaa ttcgtggagg cccgatgact cccattgaaa gcactttgcg tagtgtgcga
     3961 gtactccatt taatttttct gctgacagtt gcgttgttca tcatcaattt gcgtatgttg
     4021 cagcccgccg agcgcggaat ctccttcctt actgtatccg tctttgtgct cttgtgcctt
     4081 tcggacatcg ctattgccat ttcctttcgg gcacggatga ttacaccatc gatagagcga
     4141 ctgcgcgtta accctcaaga cgctgcagca cttgggcgtt ggcgatctgg cgtgatcacc
     4201 tcatttacgt ttgcggaaac cgtggtgctc ttgggattta tgttgaagat tctaggcgcg
     4261 ggctggaacg tcgcgggtgt cttttttgta gttggaatcc tcttgctgct tgcatgggca
     4321 ccaaaactgg atatgtcccg cgggaattag aaagagcacc aggctcgctc tcccccgcca
     4381 gtcactagaa cttatctcat aaatagtttt gtggccggaa agccacaaga cttgtacaac
     4441 gggaaccggc ggaaagtgat gattcggctg agcttttaca gtttcacttc gtaagtgccc
     4501 tcgtttctgc ggcctgcgag catttatgag atggcttcta gtcaccagcc accgttagta
     4561 cgcccagcgc aacagcacgc cgccggtcgt gtaccccgca cctacagtga cgatcagcac
     4621 cagatcgccc ttgtgcaggc gcccctcttc caccgcgtcc cgcaggccca ggggaatcgt
     4681 ccccgctgtg gtattcccat atctgtcgat gttcaccatc accttggaat cgtccatccc
     4741 cagccgctcc tgcgtcgctc gaataatgcg caggttggcc tggtgcggca ccaacagttt
     4801 tagatcgtca tttgtaaaac cattcttttc aagtagatgc actgccacgt ccgccattcg
     4861 ccgcaccgcg tacttgaaca cctgcgaccc ttcctggtgg acatagtgca tgcgcttctc
     4921 gacggtttcc tggctggagg gatggagcga cccgccgccc ggcatgtaga gaaacgctcc
     4981 gcctgtgcca tccacgtcgt gctcgaaatc gaggatcccc tcgtttgtgc cagcaggttc
     5041 gagcagcacg gcccccgctc catcgccaaa cagcacacac gtggcgcgat ccttgtagtc
     5101 cagaacggaa gtcatcgtat cgcttccgat gaccagcact ttcttgtgcg tgcccgcgcc
     5161 gacgaactgc ccgcccacgg taagcgcgta ggcaaagcca gagcacgccg cggacaagtc
     5221 aaagccccac gccttcttcg cgccaatgcg ctgctgcacc aggcaggcgg tggcggggaa
     5281 aaacatgtcc ggggtaacgc tggcgagcac gatcagctcg atctccgcgg gatccgtctt
     5341 ggtctgcgcc agcaccgccc tcgcggcttc cgtcgccatg tgcgaagtgg ccatgccctt
     5401 ctccacaacg tggcgttccc ggatgccggt gcgcgtgcgg atccattcgt cgttcgtctc
     5461 gacgattttc tccaggtccg cgttggtcat cacttttggc ggaacgaagc cggctacacc
     5521 cgtgattttt gccgcgatgc ggtgggacat gactctcctt gcgaatggct tcccgaagca
     5581 aaagaacctg aattttatgc gattgcgccg tggggataca aggagcagtc cggttctctt
     5641 cagaaccgca ggaggtaact caacttgatg aagaacagcc ggctggtggg attgtttggc
     5701 gcgccctgtg ccgttacaaa aggtgatgcc cccggatgca gcgtgaggtc gctgtaacca
     5761 ttgttgtaac caagatacac cgccgttcca ggatgcaaca gatacgtcag aagcacatcc
     5821 gtcgtgaatt gcttagtcgg cgcgaaaggc ccgttcacgt catcagagtt gccgaggctc
     5881 ctttgcagat cgaaaagatt cgtgttcgcg atcgttgcgt tgtaatccag aatcaaccgc
     5941 agcgacaact ccttcgtgaa ctggtaattc agcttcgcgc gatttaagta gttattgaaa
     6001 acgctttgcc cgggcgcgaa cggcggcgtc acccagcccg cgcgcgttcc cagccggtag
     6061 taaatcagcg cctcatcaaa tcggaaacgc gcggaaggcc gcagcgtcaa cccgaaattc
     6121 acgcgccgcg tatttcccag aaacggctgg aacggcacca gcttctgagc cggaaagaaa
     6181 ttctctccga tgccctgcgt gtatctggtg gaaaaggaaa tccatttgta cggctgcgtg
     6241 gacaccagga atgacgtgct gtgcttccgg aaaccgatag tgtcgaaaag ttcgtacgct
     6301 tctccacggc tgacgctgat ggtcgtctgc ttcgtcaaat caacttgaaa accgagggca
     6361 accccccagt cctgcaggat gcctgcgtga ttccagtcca ccgtctcggt gatggtgggg
     6421 ccgaaatcaa tgatcttgtt tttatccggc ctccagaaat aaccgccgaa cgcgttgttc
     6481 tggcggatgt cgatgcgatt gacgaatccc atctcggtgc agaaatttgg cgcgtagtcg
     6541 ttatagttgg tggaaaaact aaaatggcgg ccgctgtagg atgcgtcggc ccacagcgca
     6601 ttgccctgtt gcgagcccat tcctttcgcg aaatccgcga acgacaaaca atccaccccc
     6661 gcttttaagt cttgccgcgt ccagctgtgc actccctgaa catcgacgac ccagttcttg
     6721 aagaatttca gccgtgcgtc gagcgaagcc acgcgattgc tggtgtccgc gaaatcgcgg
     6781 gtcgaaaaaa acgcgccaat gtatgactga tttccgaatt cgcgagtgac gcgcaccacc
     6841 ccgtccgctg cccgcgtgtt gtaggcgctg ctagtggaat tttgccccgg ctgccggtcg
     6901 tcgatcacca gggcgccgag cgtccatttg ccgagcttcc ccgtcacgcg agtgccaaac
     6961 tgcggatcgg cgatctggcg cgagaaaaaa aggttgatcg gcgtcatgaa aaatccggcg
     7021 ttctcaatga agaacggccg cttctccgga aaaaaaaccg cgaagcgctg gttgagggtg
     7081 acctgcgggt cgtcggattc cacctggctg aaatctggat tcagcgtgac gtcgaaggtc
     7141 agcgaatcct tggccacaaa tttcgcgtcc agtcccgcgc ggtgctcgaa tttatccacg
     7201 aacccgggag gaccgccgct cggctgattc aaaaagtggt cgttggcaag cagtccataa
     7261 ggaatgagct gaatgttccg ccctggagac acattttcca ggccaccaac cggcgcaaat
     7321 tgctgcgtca acccttggac gcgctgcgtc acatagggcc agtagtcgta ttcgcttttc
     7381 cgaagaatca cgcgatagag cgccactccc caggtttgct ccggctcgct tgagaaccgc
     7441 aggctcttga acgggatcga aaagaagaca atgtagccgt cgttggtgac tcgacccgcg
     7501 ttatcccaca gcgtgtcgaa gctgaagtcg tatccttgcc cttcggtata gatcgcatcg
     7561 gtctgtaccc cgagcggatt ggagtaaaaa aaatacgcgc ggtgaaaatc ccgaaacgtg
     7621 tccagcagga cccccacgcc gtcgtcctga tcggtggcct cgcgcttgga aagatgcgct
     7681 cggacctcgc ccgcttcgtc gtggcaaatg aaggcggcat agagattctt gtcgtcgtaa
     7741 gagaggtaaa cggtagtgga ctccgtcgcc ggcgtgccgt cgccgggagt gttctggcgg
     7801 aaatcggtga ccgtcaattc ggcttcgcgt ggatgatttt ccaggaaatc ttccagtttg
     7861 ggcgcgcgat gtacgcgggg aatcttcgac agcggaatct ttgcctcatt tggaggtggt
     7921 gccaccgtag cctgcgcgga aacacgcgtc gccgcggcga aaacgaaaat aattccaata
     7981 atagaattcc ggatgacagg gcccatctag gatgtcttta tatagcttca actgttcctt
     8041 cgcgcaagtg caaagtggta cagatatcgt ttcgttgagt acgctagagt caaaggctgg
     8101 cggaatcctc ggcccgactg ctaggaggcc ggaggaagaa tttcataacg ccaggaaagc
     8161 gtcgccgttt tcctgagcat ggccgcaacg gagcaatact tgtcctcact gagttggatg
     8221 gcctggttta ccgccgcgtc gtcgagatgg ccgcggagtc gatagagcat ttccagcttg
     8281 gtccagaccg tgggcggctc cgccgcacgt tcgcccgagc aaatcacttc cagagattcg
     8341 agcttctgcc gcttcttttc caggatgatg actacgtcgg tggcggtgca ggcccccagc
     8401 gccagcagca caagctccat cgggccgggc gccttgttcg aagcacgatc ggaatcgatg
     8461 gtcatcgcgt gtccggacgg gctggtggcc acgaatttct gttcgccaat ccacttcacg
     8521 gatccagtct gcatgtggcc tccccggaga aagcatagca ggaccgggaa cttgaaacga
     8581 gcgacgcaga ttcccattgg ctttcgattc gttttttggt ttaaatggcg tggtacggtc
     8641 ggtagtctca atagcgttcg gggaggagag aaccaatgca aatccaagtc tcaaaggtaa
     8701 agcgcaagct cgcaatggct gcgcttgggc tggcggcgct tctttggtca tcgacaatcg
     8761 gagccggagc ccagcagggc gttgtggctg ccgacggaaa agatcaaccg tacgtgattg
     8821 agtactacta caaggcgaaa tggggccacg ccgaagaatt cctagcgctc ttcaaaaaga
     8881 accactaccc ggttctgaaa aaggaaatgg agctgggccg catgctaaag gtcagcatga
     8941 ccgttccgcg ctatcacacc accgaagacg gccgttggga ttaccgcgtt actatcgtct
     9001 tcaagaacgc cgcgatcgcc aacgacaatt ttgacagctc cgctatgatc aagcagctct
     9061 atcccgatca ggaaacgtac aagagagaag aacagcgccg cttcgaaatc ctagaagcgc
     9121 actgggacct gcccatcaag gacgtggacc tcgaagcgcg ctgatcactt aacgtggtgg
     9181 aagagaggat cagttgcccc gcaccgggcc ggtggtcgcg gcaacttcaa tgatcgggtt
     9241 aggcagcgcc cgcatggtgc ggacgaagag tttcacgatg gttccgtcaa actgcgcgtt
     9301 ggcgcagcgc tccagctcgg cgagcgcgtc tgtcgcactc cgggccttct tgtacgtgcg
     9361 gtcgctggtg atcgtatcgt aggcgtcggc gaccgcgatg atgcgagccc ctagcggaat
     9421 tgtctccccg gtcaatgcct ctggatatcc cgagccatcg aagaattcgt ggtggtgcag
     9481 taccatttcg cggatgcgcg cgagcggcgt cagaggatcg aggatcttag cgccaaactt
     9541 aacgtgtgac ttcatggtct cccactcttc gggattgagc gggccgttct tgttcagaat
     9601 gcttcccggg atgccaacct tgccgatgtc gtggagcacg gcgcccagac gaatctcttc
     9661 gacttcggcg tctttcatcc caatcgcttc cgcgatcagc gcggcatacg cggagacttt
     9721 ctgcgagtgg ccttgtgtgt agtgatcctt ggcgtcgatc gcaaacgcca gcgaggtgac
     9781 cgtgtccagg accgcttgcg gcagtgcttg cgggccctgc gccggggtcg ttgctgggtt
     9841 gccgttattc ggttcggtgg ccgcgagcga ttcggtgaat tgccgcaagc gctggtagat
     9901 ttcttcaaac gcctcaggcc ccgtcgagaa gagccgtttg agcgtcacgc ccagatacgc
     9961 ttcaaggacg tcacgcttcc atttcttagc ttcgttggga tcgaagtgat cggcggttga
    10021 gacggcgttg ccgccctgat gtttcgaaag atacatcgaa gcgtcggcca cttggatgag
    10081 ctcttgcggt gatgagccgt ggagcggata acacgcgatg ccgaagctgg cgctgatgtt
    10141 tttctcgcgc agcaacggat cagcggatac ccagccgcgg agcttggtcg ccagttgccg
    10201 cgcatgctcc atattggttt ccggcatcag aatgacgaac tcgtccccgc cgtagcgggc
    10261 cacaacgtcg gagcgccggc agttggtctc cagtatctgc ccgacgcgct gcagaaccag
    10321 atcgccttcg aggtgcccgt agaaatcgtt cacgaatttg aagcggtcga ggtccatgag
    10381 caccagcgca aaggcgcggc cggctcgagt ggaccgcttc cactccgcgg acaatgcttc
    10441 catgaaaaaa cgatgcgtct ttacgccggt gagtccgtcg gtgatcgcct gttcctgcgc
    10501 cttttggaag gaaagagcgt tgtgcagtgc gccggcgatg aggtcggcca gcgtgcgcag
    10561 caggagaact tcctcctcgg agaagtccac gggcacggac gactcgatgt acagaatgcc
    10621 gtggagttgt tcggcgtaaa aaaccggcaa ggcgatggcc gccacggaat ccggcaagag
    10681 cggcttcaac gccccatcgg ccggggaagc ggcgcggtat accgccatgt ggccgttgcg
    10741 agccacgtgg ccgatcaagc ccgcgcccag ggaaatgcgc tgcccgaggg tcccgcggcg
    10801 ttttcccgct tcggcttgga tcacgacttc gcgcgtcgcg tagtccagca cgccgatgcc
    10861 gatgtgatcg tagatcagcc cggcttcgag ttgttcggtg atcttggcga gcatctcgtc
    10921 cggattgagc gtggcgatgg cgtttcgcga aatcgtgttc agcagcgaga gatgccgcgc
    10981 cttggtgcgc tcctcgctga acagccgggc gttttcaatt gcgatactcg cttcgcttgc
    11041 aaggacgcgc agaagctcca ggtgcccttc gtcaaacaca cgctcgtggt cggagtacac
    11101 cgccatcgcg ccgatggcgt gatcgtaggc caccagcggt acgccgcaga agcacccttt
    11161 actgcgcagc ggttcgacgc ccagtttttt catttccccc acgtaattgt cgcggatgag
    11221 cacgggctgg cgcgtgcgaa tgatgtactc ggtgatgtgg ttgcccgcgg ggcgcgcgcg
    11281 cttcggcagt cgcgcgccat ccacgatctc gagatcgaat tgaatttcgt cacgcagcgg
    11341 atccagctcc gcaatgtaga agttttccac gtcgaagagg cgccgcaact cttcccacac
    11401 tttcctcgtc aggtcttctt tgttcagcgt cgagctaagc gcgcggccga tttcgttcaa
    11461 tacgtgaagc tcttcggacc gccgcgaagt ctgctgaatc aagtagctgt tctccacggc
    11521 catgccgatc tgatgcccga gcgcgaggag aagccgcaac tccgccggtg tgaagcgccg
    11581 gctgtccggt gtcccgagaa ggagcacgcc gaaggcttgc tctttggctt gtagactgat
    11641 ggccaccacg ctgcgaagcc cctgcgcgag cgccagctcg cggaatcggc ggatggactt
    11701 ttctttttcg agcgccgaga gggagtcgtc gcgaaggtca cgaaaaccca aaaggccgcc
    11761 gagtcgcgcc acgaggttga cgagataatc gtccagacct tcgtcctgag cgacgcggca
    11821 gaactcatca ctcaagccta cggcaacgac ggaagtcggg ccctgcggat caccgtggtg
    11881 cagaaagagc gctccggccg gcagccgcac aacgcccagc acgcggtcca gcgcctgcga
    11941 gagcatgcgc tgaatctcgc cgcccacgaa gctggaggtc gccagattga gattcgaaag
    12001 cgccagcatg ttgcgctcga tgcggcgttt ttcttcttcg tatagcgcca tgatcatgag
    12061 catggccacg aacgcgcagg gagcagcggc aaccacgctc atgcctgcgc cctcgagttc
    12121 cggcgttgcg ttcagggtat cgagcgcgat ccacagaact ccccaggaag cgaaggagac
    12181 acccagcagc cggtcggcca gcgtttcctg ccgccggctt tcctgccaga acacgaccgc
    12241 aaccgcgatg tacagcagag aagcgggaaa gatcgcggaa accgaaatcg cgttggtggc
    12301 gttgcctgcg gcccagaatc cgaggagtac gcccacgacc agcccgggaa ttttccaggc
    12361 cttccgctgg gagtaaagct gtgcccctag aaaaaagagc accccggaaa gcgcgaacag
    12421 ccaatgattc agcgcaccct gcagcgggct cgctgctgta ctgggagaga gtgtcggccc
    12481 cacgtaatgc agcccgaata cggcccacgc cgcggtccat agctgcagat acgtctggcg
    12541 cttgatgctg tggatatacg cgaagaagcc cgccaccaaa gtggtggcga tcagcagcgc
    12601 cagtgagata aaactctgat gcatcaggaa tactttcgat tctccgcccg catcgcggac
    12661 ggcacactgc cactcacgaa ggcacaaaaa tccttcgtgc tctgccgtgt atccgcaacc
    12721 ctgctacctg cacgagagga cagtcgtgcg gcgacgcagg aaggtcgcgc ctgtacacaa
    12781 aacaaagtac ttgtgattat ggattacgga attttcggga agcaagcgta ccagggtact
    12841 ggtggccaaa gagacagcct cccggaggta accgattcaa tacaaagggg catagcgcgt
    12901 tccgtccccc tggcccatat cccgccaatt ggacctaaag agtgggtttg gctacactga
    12961 gagtttgaat tcaagtggaa agacgcgctg acaatgagtg atccgattca caagacatct
    13021 tccgtgagag tgcgatttgc cccgtccccc accggttatt tgcacgtggg aggagcccgg
    13081 accgcactgt ttaattggct attcgcgcgg cgccagggcg gcacgatgat tcttcgcgtc
    13141 gaggacaccg atgccgagcg caacaagccc gaactagtgc agggaattct ggatggtttg
    13201 cagtggctgg gcgtcaattg ggacgagggg ccattttacc aatcgcagag aacggaattg
    13261 tatcgcgcgg cggcgaaaaa atgtttggcc aatggggctg cattcctgtg ctactgccct
    13321 acggaaaaat acgccggcgg cgatcacgcg gaagaaggca gcgaagccgc gcagttgcgc
    13381 cgcgcctcgc gttgttcctg ccgcggcggt ggaaaacctt cgaacccgga tgtgaagccg
    13441 gcggtccgct tcaaagttcc tcttggtgaa acaacgaaat tcctcgacgc ggtattcggt
    13501 gaacgcgagt tttcgaacga tgaaatcgag gactttgtcc tgttgcgctc cgggaaaagc
    13561 gacgaagaat tcggccagtc catgtatcag ctcggtgtgg tcgtcgacga catcgacatg
    13621 cgcatcacgc acgtgattcg cggtgcggat cacatttcga acactccgaa gcaggtgttg
    13681 ctgtatcgcg cgttgggcgc ggaaccccct gtttttgcgc atgtgccgct gattctcggc
    13741 gcggacaagt cgcgcctttc gaaacgtcac ggcgcaaccg atgtaaatat gtaccgcacc
    13801 gaaggtttcc tgccggaggc gttccgcaat tttctcgcgc tattgggctg gtcaccgggt
    13861 ggagacgaag aattcttgcg caccaaggat ttgctcgaaa agttttcgct cgcgggcgtg
    13921 aaccgcacca acgccgtatt cgaccggccg aaactcgagt ggttcaacac gcagtactta
    13981 cagaaacttc cgatcgaaga gttgctgccc gaagtcgaaa atgaattgaa gcgcagcggc
    14041 ttgtggaagc cggagtgggc ggcgggagcc gatccgggcc ccgacggcag agatcacgcg
    14101 tggttttcaa agaccgtcga tttgctgcgg ccgcgaatcc gtttgctgcc ggatttttcc
    14161 tcatggtcgc acgcattttt cagcgacgaa ttcactctcg atgcggcggc caaagaaaaa
    14221 ttctggaaag atccgaaggt gccggaactc ctcgcgaagc ttgccgatgc gctcgaagcg
    14281 cttgccgagt ggaatcacga cgcttgcgag cacgcctccc gcgccgtggc cgaaaccggt
    14341 ggcgtcaaag ccggactgct gatcaatgcg acgcgcgtgg cgatcgtcgg tcaggtcgtg
    14401 gcgccgccgc tcttcgacac catggtcgtt ctcggccacc agcgtgtcgt ccgccgtttg
    14461 cgccatacgg tggccggttt cagtaaagcc tagagcaagc cccccttcaa aaaacctcga
    14521 agtctaccgc cctagtcgaa aacccatttt ggggattggc acggcaggct tattgttttc
    14581 caaaaatctc cgagtgggcg ggcttggcgt cggaggctgc agcggcgaca ttgagcgttg
    14641 cgtcgagcgt gagcgtgacc ggaggcaggc acagttcgtt gctgcaagcc tggtagcgga
    14701 gcttcagcgg aatgtgctgt tcgccgagag gggcactctc gagcgccgtg acgggcatgc
    14761 gaagaatcac ggtgtcctgg tagacgttca gcggagtttt ggaaaacgag aatttctcga
    14821 gtttgccctt gggataggag acttcaccgc cgatgaagcc ggccggaagt gcggccttga
    14881 gatcggtagc gatgaggtag tcctctgatt tttcgcgggc gttgacatgg aagccgggac
    14941 gaattttcat gaccacggca atctggaaag agctgccgcg gccggcggga tcgagcgaaa
    15001 cgtagatttt cggtttgacg acatcgcggc cggaaggaat ttgcgaaggc gcggcgggtg
    15061 atagcacgac agacagtgcc aagacagtga tgaagaaaaa tgtctgggcg gatttcacag
    15121 ggtcacctgg gcgccgagtt gctgggagat aacgcggctc atgacggcgt ggagatcttc
    15181 gccggttttt tcatgtacga aggcgttgcg cgattcgttc cagcccagct tgaaactggt
    15241 ggaaagcgcg gagatccaga tttcgcggac gggcgtgttg ggcgaaatca cgaacttgac
    15301 ctcctcgggt tcctcgaaga gaacttcgag cttaccgctt tcgccttcca cgtcgaaacc
    15361 atgttcatcg ccagcggcga gcatgcgtcg cttgaggtct tcgaacgcgg cgtcggcctt
    15421 gcgttgaaag tctttttcgt caagcatgca tttagcgtac atgaagagag tgagtcgtgg
    15481 gtagtggact gtgggggaag agacaaagca atgagctgag ggcgcaatga gataaggaag
    15541 caggcgggaa tggggccctc cgtatcgcgt ccttcgaggt tcgacgtctg gttctgtgag
    15601 aaaatccggg ccagaaaaaa tgttctgtgc ggagtattga gcagcggccg gtattaatgt
    15661 cctatctgca agggggacta tggtcctatt gctcccgatc gcaggaaaag agagagtgga
    15721 cgtagcgtcc agagatcctc gagttccaga gcctcagcgt gtggacctgc gccggtctaa
    15781 aattgttcta aagtggaact tttatgaatc tatttaggaa gcccgaattg cacttgtccg
    15841 catcgcgcgt ttttcgcggc tcacgatgcg gtgcgatcga actgaaatga agcggctggc
    15901 acattggcaa ctggcggcgg tggccgggtt cctgattttg tacatcatcg tttcgctggt
    15961 ggcgccgcgt gggtttgcgc tgacggcgtt ctccgatatt accggcacag gattgtgggt
    16021 gatcgccatc gcggtgatgc tgtgggcggc gttcagtaac cagggacgaa cgcggtggtt
    16081 ctggatcctg ctggccgcag gcgcggcgat ggtttgcagc aaccttgcag cgtggcttta
    16141 ttacgaggtc atcgcaggcc gaagcccacc ggacccgttc tgggcagaca tcccgctttt
    16201 tctgcaaccg gttccgatga tgaccgctgc ggcgatgcgg ccgggttcca gacagcaaga
    16261 gcagaaattc catctcagca cgctgaattt tctgattctc ctgctgtggt ggatctacat
    16321 ttatatgttt ctggtgtatc ccaacgaata catcctgcca aacaagatct ccttcaatag
    16381 ctattactac acgctgtttg ttctggagtt cgcggtgctg caggccgtac tgggagggat
    16441 ggcgctggtt gctaaagggg cctggcgcaa gatctactgg catctttttt tcgccggctc
    16501 gctatacctc cttgcctacc agtggctgaa cgcagccctg ggacgcgatg aatactacag
    16561 cgggagcatc tacgacgttc cgaactacgc ggccatttgc tggttcattg tgattgcctt
    16621 gcgcgcgcgg aatgtgcctt ccgaaccgct ggctggcggc gaacgggacc gctgggccga
    16681 cgtgcccgga tttctggccg tgcttgcagt cctgtccttg ccggtgatcg gtctgtgcga
    16741 gctgtttctg gatccgcagg ccagccagtt gcgcccgttt cgcgtcttgg tcaccctgat
    16801 cgggatcctg ctggtgggcg tatgcgtctt tctgcggcaa atcctgctgg cgcgggaaat
    16861 ggcgcggctg ctgctcgatt ccaagcagaa tctggagcgt ctgcaacgcg cccaaagcca
    16921 gttggtgcag aaggagaggc tggcgggagt tgggcaattg gtcgccgggg tggctcacga
    16981 actgaacaac ccgctgactg cggtcatggg ttactccgac ctgctcatcg accaggctgc
    17041 ggagggcacg tctcgcccca agctggaaaa gctgagagcg gaagcgcagc gcatgaagcg
    17101 gatcatcacc aacctgctta ccttcgcgca accccagcat gaaggccgcc ggctgttgga
    17161 cattgccgtg gtggtgcgag agtccctgat gctcctggag tatcaattgc ggaactgcgg
    17221 gatcagcgtg gaaatgaatt ttgcgcccaa tcttccgcgg attgctttga atgaggggca
    17281 attcaaacag gtcttcgtga atcttttcag caactccact caagccctgg aacaatcgca
    17341 agaaaagaaa atcttggtgg aggggtattt ggagggcgag aaagtcatcg tacggttcac
    17401 cgattcgggg ccgggattca acgacgttag ccgtgcgttc gatcctttct ataccacaag
    17461 gcccgtcggt cagggaaccg gtttaggcct gagcatctgc tatggcaccg tccgggaaca
    17521 taacggcaac atctacgcgc aaaacttgca gcccaacgga gctgccgtga ccatcgaatt
    17581 acctcttgga tgaggttaga aagcatcgac tggcccacga aaccggtggc agcattctcg
    17641 cagcctgata agttttctcc cgactgctct aatcgccacg caaaaattgg tttccgctgc
    17701 aaggctgttg atctcgagga cgggatggct ctgttgacat gctcggcaca gtgcgcgttt
    17761 tgaggggtcg acaagcggag ggggagcgtg ttttctgatc aagcaggagg gacgatggcc
    17821 gagatcggac tcctgctttt tgctcgcgtg gccttgcaag tggccacccg ggttttgccg
    17881 ccctaccgca gtagg
//
LOCUS       DASYVO010000247         5245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_119558, whole genome shotgun sequence.
ACCESSION   DASYVO010000247 DASYVO010000000
VERSION     DASYVO010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119558"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VGR03_08255"
     CDS             <1..712
                     /locus_tag="VGR03_08255"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEV1994308.1"
                     /translation="GMQTKVIEPVMVLIREGWFGMGCATGRDDEKPAHRVWVDAFEMA
                     AYQVTNEEYGCFLAATSAAPPTCWGDANFSDPKMPVVAVSWHEAASYGEWLSRATGKS
                     YRLPTEGEWERAARGGAEGFSYPWGDAPPEMVPDYGKRWKLGPEPVGLYAPNAYGLYN
                     LGDNVHEWCADWYDDGYYGRSPERNPPGPTSGSRRASRGGSWRHHIKVTRTAARSSIP
                     PEFQYADYGFRVARSPTT"
     gene            822..1619
                     /locus_tag="VGR03_08260"
     CDS             822..1619
                     /locus_tag="VGR03_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994309.1"
                     /translation="MRHGSSALRLIALLVAVVATAAAQQEPKPRDLPDAPVAKQESTP
                     QKRQNPFNTTIEVLGRRSIFFPDIATSVGPLSSKQKFELFADESVAPSRFLSSAFGAG
                     IGQAQNSLDGYGQEMGGYGKRFGSSMATAASNNFFGTFLISSLLHRDPRYFVTLHGGA
                     GHRIGYALSRIVVARTDDGKDAANWAGMLGPLLAESLANSYLPVKEQTAGRTFQRYGI
                     RIGLNTASNALREYWPTIFRNLRITKIAPGLKSNPAATAPGATRPSP"
     gene            1670..1746
                     /locus_tag="VGR03_08265"
     tRNA            1670..1746
                     /locus_tag="VGR03_08265"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1704..1706,aa:Arg,seq:cct)
     gene            complement(1818..2546)
                     /locus_tag="VGR03_08270"
     CDS             complement(1818..2546)
                     /locus_tag="VGR03_08270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1994310.1"
                     /translation="MESLKDQIAVVTGASSGIGKAIALSVAGQGAEVCLVARRRELLE
                     DVAKQAHALGSPAHACPADLTKDEDIRALGERFEKDFGHVNILVLCGGAIFHGPLEKA
                     SLAEFDLMYRSNVRGHYLMIQTMLPLLRKAAGQIVFINSSAGLRSPATAGQFSATQHA
                     FRSIADSLRDEVNALGIRVLSVYPGRTATPRIAKLFEKEGRAYQPDLLMQAEDIAEMV
                     THSLRLPRTAEVTDISIRPMQKSY"
     gene            complement(2644..4014)
                     /locus_tag="VGR03_08275"
     CDS             complement(2644..4014)
                     /locus_tag="VGR03_08275"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HEV1994311.1"
                     /translation="MKYDAIIIGSGQGGNPLAYRLADLGWSLALIEKKDLGGTCINVG
                     CTPTKTMVHRAQVAHYVRNAARWGVNASNVSVDLAKIVAQKDEVVLSFRGGQQKQVDK
                     RANLRLHRGHARFVGSHQLKVGDDLLESEKIFIDTGGRPNIPAIPGMDTVSYLTNESI
                     MQLTTLPEHLLILGSGYIGLEFGQMFRRYGSRVTVLHTGKQIVPREDPEIAVELQKAL
                     EAEGIQFLLNARTTRVENKNSAATLSFEGPAGSSSVTGSHLLIATGRGPNTDDLGLDK
                     AGIETNKNGFIKVNGRLETNIHGVWALGDCKGGPAFTHISYNDFQIVYGNLVEGKNLT
                     TENRPVPYCVFTDPQLGGVGMTEKEARAKGFKLKIGKCPMTYVARAIERGETAGLMKL
                     VVDASNDRILGASILASEGGELVQILGTLMLANQPYTLLKGAVYIHPTLAEGFFALME
                     DVKPAD"
     gene            4788..>5245
                     /locus_tag="VGR03_08280"
     CDS             4788..>5245
                     /locus_tag="VGR03_08280"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEV1994312.1"
                     /translation="MSSRRGFLQNVLAGAGIFASAKVLSAQEMQMSGDIPGMNMKGKK
                     GSIHDHGSPVLVETPDVPNLPWRMDGNVKEFHLIAEPVKQEIVPGRLVDLWGYNGSAP
                     GPTIQITRGDRVRVIVDNDLPEATSMHWHGFDIPNDMDGSPGSSQDPIAPG"
BASE COUNT         1095 a         1417 c         1646 g         1087 t
ORIGIN      
        1 cggaatgcag acgaaagtca tcgagccggt gatggtgctc atccgggagg ggtggttcgg
       61 gatgggatgc gcgacggggc gggatgatga gaaaccggcg catcgggtgt gggtggatgc
      121 ttttgagatg gcggcttacc aggtgacgaa cgaggagtac gggtgcttcc ttgcggcgac
      181 gagtgcggct ccgccgacgt gctggggtga tgcaaacttc agtgatccga agatgccggt
      241 ggtggcggtg tcttggcacg aggcagcgag ttacggcgag tggctgagca gggcgaccgg
      301 caaatcttat cgcttgccga ctgaagggga atgggagcgc gcagcgcgcg gcggcgcgga
      361 aggcttttca tacccgtggg gagatgcgcc tccggagatg gttccggatt acggcaagcg
      421 gtggaagctg ggaccggagc cggtgggttt gtatgcgccg aatgcttacg ggctctacaa
      481 tctcggcgac aacgtgcatg aatggtgcgc ggactggtac gatgacggct actacgggcg
      541 ctcaccggaa cgaaatccgc cagggccgac gagcggcagc cgccgggctt cgcggggcgg
      601 ctcgtggcgg catcatatta aggtgacgcg gacggcggcg cgttccagca ttccgccgga
      661 gttccaatac gcggactatg gtttccgggt cgcgcggtca ccgactacct gagaatgcag
      721 gagcggtttg tttgccccat accgaaaatc acgcgcgcaa agtcggcgcg tggggcacga
      781 gggagcaaga acgagaattg ggttcatgtt aggatgagag gatgcggcac ggttcgtcgg
      841 ctctgcgctt gattgcactg ttggtggccg ttgtcgcaac ggcggcggcg cagcaggagc
      901 caaaaccacg agaccttccc gatgcgccgg tggcaaaaca ggaaagcact ccgcagaagc
      961 gccaaaatcc cttcaataca acgatcgaag ttctcgggcg gcggtctatt ttctttcccg
     1021 atattgcgac cagcgtgggc ccgctgagca gcaaacagaa gttcgaactt tttgcggatg
     1081 agagcgtcgc tccttcccgt tttctcagtt cggccttcgg tgcggggatt ggccaggcac
     1141 agaattcgct ggacgggtac ggccaggaaa tgggcgggta cggaaagcgg tttggttctt
     1201 cgatggcaac ggcagcgtcg aacaattttt tcggaacgtt tctgatttcg tccctgctgc
     1261 accgcgatcc gcgctacttc gtaaccctgc acggaggcgc gggacaccgc atcggttacg
     1321 cgctgagccg catcgtggtg gcgcggacgg atgacggcaa agatgcggcc aattgggcgg
     1381 ggatgctggg cccgctgctg gcggagagcc tggcgaacag ctatttgccg gtgaaagaac
     1441 agacggcggg gcggaccttt cagcgctacg gaatacggat cggcctgaac accgcgagca
     1501 acgcgctgcg ggaatactgg ccgacgatct ttcgcaatct gcgcatcacc aagatcgcgc
     1561 cggggttgaa atcgaatccg gcggctacgg cgccgggggc gacgcggccg tctccttgac
     1621 gaagacagac ggaaccgaaa aagcgcggga agtattacaa tggaagagcg gggctgtagc
     1681 tcagatggat agagcaacgg tttcctaaac cgtttgtcgg gagttcgaat ctccccagcc
     1741 ccaccagaat tagaaattgg aaataagaaa ctagacaggc ctcgcgcaac ttttgcagaa
     1801 gcgtgcggaa ggcttagtca gtaggacttc tgcatcggcc ggatgctgat gtcggtgact
     1861 tccgcggtgc gcggcaagcg gagcgagtgg gtaaccattt ccgcgatatc ctccgcttgc
     1921 atcagcaggt ccggctggta cgcgcgacct tccttttcga acagtttcgc gatgcgcggc
     1981 gtggccgtgc ggccggggta aacgctcaga acgcggatgc cgagagcgtt cacttcatcg
     2041 cgcaaactgt ccgcgatcga tcgaaacgca tgctgagtgg cggaaaactg cccagcggtg
     2101 gcgggggagc ggagtcccgc ggaggaattg atgaacacga tttggccggc ggctttgcgc
     2161 agcaggggca gcatggtctg aatcatgagg tagtgaccgc gcacgttcga acggtacatc
     2221 aaatcgaact cggcgagcga agccttctcc agcgggccgt ggaaaatggc gccaccgcag
     2281 agcaccaaaa tattcacgtg gccaaaatct ttctcaaacc gctcgcccaa ggcacggatg
     2341 tcttcgtcct tggtgagatc cgcggggcac gcgtgggcgg gcgagcccag cgcgtgcgct
     2401 tgcttcgcaa catcttcgag tagctcgcgc cgccgagcca ccaggcagac ctcggcgccc
     2461 tgcccggcta ccgaaagggc aatggctttt ccaatgccgc tgctggctcc ggtcacgaca
     2521 gcaatttgat ccttgagact ctccatcact tgtgttgtag cctccgccgg cttgtggccg
     2581 aaactgtagt ttgcagaata ccattggtgc aactcagcgt cgcagtgcga agcgacattc
     2641 cggtcagtcg gctggcttga cgtcttccat aagggcgaaa aagccttcgg cgagcgtggg
     2701 gtggatgtag acggcgcctt tcagcagcgt gtagggctgg ttcgcgagca tgagagtacc
     2761 gaggatttgc accagctcac ctccttcgga tgcgaggatg gaagctccaa gaatgcgatc
     2821 gttcgaagcg tcaacgacca gcttcatgag ccccgccgtt tcgccacgct cgatggcgcg
     2881 cgccacgtaa gtcatcgggc atttcccgat ttttaattta aaacctttgg cgcgagcttc
     2941 tttctcggtc atgccgacgc cgccgagctg agggtcggta aagacacagt acggcacggg
     3001 ccgattttcg gtggtgaggt tcttcccttc caccaaattc ccgtaaacaa tctggaaatc
     3061 gttgtaggag atgtgcgtga aagccgggcc acccttgcaa tcgccgaggg cccaaacgcc
     3121 gtgaatgttt gtctcgagcc ggccgttcac tttgataaaa ccgttcttgt tagtttcaat
     3181 cccggcttta tcgagaccga gatcgtctgt atttggacca cgcccggtgg cgatcaagag
     3241 atgcgagccg gtgacgctcg atgagcctgc gggtccttcg aaagacaatg tggcggcgct
     3301 gttcttgttt tcgacgcgcg tggtgcgggc gttgagcagg aattgaattc cctcggcttc
     3361 gagcgccttc tgcaactcga cggcaatttc cggatcttcg cgaggaacaa tttgtttgcc
     3421 ggtgtgaaga acggtgacgc ggctgccgta gcggcgaaac atttgcccga attccaggcc
     3481 gatgtagcca ctgccgagaa tgaggaggtg ttcggggagg gtcgtgagct gcatgatgga
     3541 ttcgttggtg agatacgaga cggtatccat gccagggatc gcagggatgt tcgggcggcc
     3601 gccggtatcg atgaaaatct tttcgctttc gagcaaatca tcgcccacct tgagttgatg
     3661 cgagccaaca aaacgggcgt gcccgcgatg gagacgaagg ttggcacgct tgtcgacttg
     3721 tttctgctga ccaccgcgaa aactgagaac gacttcgtct ttttgagcga caattttggc
     3781 gagatcgacg ctgacattgg aggcgttgac accccagcgg gcggcgttcc gcacgtagtg
     3841 agcaacctgc gcgcggtgaa ccatggtctt ggtaggcgtg cagcccacgt tgatgcacgt
     3901 cccaccgagg tcctttttct cgatgagcgc aagagaccat cccaagtcgg cgagccggta
     3961 agcgagcggg ttgccgcctt ggccggagcc gataatgatt gcgtcgtatt tcatgggaac
     4021 ggtcctttct tgaggaatcg cgccaagaca atacatcgga tgcgttacga atgtccgaag
     4081 atatccgatc gcgctgcccg cgcggagtcc aatctactca tcgactgttg agaccgtaaa
     4141 ggcaggcgcg ggcgatgcaa ggagcatttt tgtacaagaa cgatctcgcg tgtatactca
     4201 gaacttgatg cgacctaagc tagtaatcgc gattctggtg ctggccctgt cgggagccaa
     4261 ttccggtgcc gcctcgatgt gcgcagctta ttgcatgtca tctgcgtctg tgggaagtgc
     4321 cgcggtccat catcaccaga tggagtccca gccgaacccc gcgagcatta gccatcatat
     4381 tcatgctcgt catcacggtg cagattgtgc ggagtgtccg cccaagtcag ggaacagcct
     4441 gaaccagaag tctgattgcg ccagcttggt gcaagttcag gcgctcaagg aaggctcctt
     4501 ctctctagat gcaccgagcg gagtcgctca gttcgatgtc gccgacacgc ctgccgacgc
     4561 tctcgcgtta gccggtggtg gcgagcgttc tatgcccttt gacgcttctc atacgatcag
     4621 aaattccaac cctgcttcag ttccccttcg tatctaggaa aaccttctaa ctagtcagct
     4681 tgcttgctcg tagacggtgc ttctgtgcat ttccgtctgc attcagtgtt cctcataacc
     4741 aatccacttt tcgaaattcg gtcattcgac cttgaaagga aaaaagcatg agtagcaggc
     4801 gagggttttt gcagaatgtt ctcgcgggag caggaatttt cgcttccgcg aaagtacttt
     4861 ccgcccaaga gatgcaaatg tccggggaca tacccggaat gaatatgaaa ggcaagaaag
     4921 gctctatcca tgaccacggc tctcccgttc tagtcgagac gccggatgtc cccaacctgc
     4981 cgtggcgcat ggatgggaat gtaaaagaat tccatcttat tgccgagccg gtgaagcagg
     5041 aaatcgttcc cggccgactc gtcgatctct ggggttacaa cggcagcgcg cccggaccga
     5101 cgattcaaat taccagaggt gatcgagtcc gcgtcatcgt agataacgac cttcccgaag
     5161 caacgtcgat gcactggcac ggtttcgata tacccaatga catggacgga tcgccgggat
     5221 caagccagga ccccatcgca cccgg
//
LOCUS       DASYVO010000248         8438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_260846, whole genome shotgun sequence.
ACCESSION   DASYVO010000248 DASYVO010000000
VERSION     DASYVO010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8438
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260846"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VGR03_08285"
     CDS             complement(<1..347)
                     /locus_tag="VGR03_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994313.1"
                     /translation="MKTAVGFANFCGRVCLVLCLFVLLARPAFAFDDPPIRVARLNYM
                     EGRVSFQPGGEPDWGWATLNRPMTAGDSLWTGNGSRAEMHIGSTAIRVGGQTSVSFLN
                     LDDRTVQIQLNSGT"
     gene            786..1976
                     /locus_tag="VGR03_08290"
     CDS             786..1976
                     /locus_tag="VGR03_08290"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEV1994314.1"
                     /translation="MIVKFSLGLCCSLAMLLVLLLPPPVIGGDIPQLVVDDDKVECPN
                     AAFSRIQDAVDAASPGATIRVCKGNYHEQVAIHKPLTIDADSGAVLMPGTMQQNTTSL
                     LDGAPIATAILVADTTDVSIEGLIVDGANNGVPQCSPRLFGIAFQNASGEISQVAIRN
                     FKLGTGLGGCQSGTGIFVQSGRGGISNVEIEKSTIHDFQKNGITANEVGTHVSVHGNV
                     VTGIGPTSGAAQNGIQIGFGAEGAISRNTVTNNLWSPCTAVDTCQAVATNILVTQSDG
                     VEVTDNDVGISQVGIFLHGNQGHVARNETFASSVFDGIRLEGDGNRVRHNQVFNGAES
                     GIFVAGNNNAVEHNKITEAAVGILKETGSLGNLIRFNQVFDTLVAVQDPALPGLAGVV
                     SPER"
     gene            complement(2317..3129)
                     /locus_tag="VGR03_08295"
     CDS             complement(2317..3129)
                     /locus_tag="VGR03_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV1994315.1"
                     /translation="MASLPSVTWSQGSAPVKRKGNIRQSVSRWCYQKIPLDKLCAYAA
                     EIGLKAVDLLNPDEFEVPGRYGLVCAMGYAFGGDIASAMNRVENHAKIEEAFRKYIPL
                     AAKAGVANVITFSGNRAGMSDEEGAENTIIGLNRVKKIGEDHGVTICIELLNSKVDHK
                     DYMCDHTPWGVQVVQAVNSPRVKLLYDIYHMQIMEGDLVRTIQKNIDWIGHFHTGGVP
                     GRHELDGTQEVQWDGVMRGIAATNFRGYIAHEFVPTRDPLTSLRQAADLCDV"
     gene            complement(3233..4117)
                     /locus_tag="VGR03_08300"
     CDS             complement(3233..4117)
                     /locus_tag="VGR03_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994316.1"
                     /translation="MPSNFAIRLRCPHGDSDSWVTLSNREESLEQILQQTWDFKCREH
                     GLQQGIPQEAMEVAPLDDPRPSQKKPPTFAVPHPTAPAKKVLRSSERIPMRVPVVIYG
                     FAGKGGAFHEDTETVIVNSSGALVTLKTKLALGDTVFLIHKSSRQEQEVRVVYLDAYS
                     DRETRIGLAFKQPISDFWKKSRKKPRIPKTLRVIVKGTDAKGHPFAQSAYTVDLSQDG
                     ARLDGVGFLTSPGETIEVRRRWRKARFRVVWIGQVGTSESNQVGVFGLQSEKDIWHVE
                     LPKTAAASPPQSPKPPKK"
     gene            complement(4231..6492)
                     /gene="glgB"
                     /locus_tag="VGR03_08305"
     CDS             complement(4231..6492)
                     /gene="glgB"
                     /locus_tag="VGR03_08305"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV1994317.1"
                     /translation="MKHTAPIAPRDPRLDAQIDALLAARHSDPFALLGPHPVDGNWAV
                     RFFLPWAAEACISLKPPSLEGATLSPAKVTDAMKLRPEGFFEATWPSNQSGPPSPGSY
                     KIHGRTHFGDSFEIFDTYAFPVVLTEFDLYLMGEGRHYDTYEKLGAHVITLEGVRGVH
                     FAVWAPSAKRVSVVGDFNRWDGRVHPMRARGSSGLWEIFVPELPEGAIYKYEVVGPSG
                     NILPLKADPYAFRSELRPNTGSVVARLDHHEWTDADWISHRAQKNWFESPISIYEVHI
                     GSWRRVPDDHDRWLTYRELGDQLIPYVKDLGYTHIELLPIMEHPFDGSWGYQTLGYFA
                     ATSRYGTPAEFMEFVDRCHQAGLGVLLDWTPAHFPRDTHGLSQFDGTHLYEHSDPRQG
                     AHPDWGTLVYNYGRNEVQNYLISNALFWLDKYHIDGLRVDAVASMLYLDYSRKPGEWV
                     PNQFGGRENLHAIDFLKRMNEVAHGKFPGVLTIAEESTSWPAVSRPTYLGGLGFSFKW
                     NMGWMNDTLKYFSHDPVHRKYEHNKLTFSLLYAFTENFLLPFSHDEVVHGKNSLLHKM
                     PGDMWQQFANLRLLYAYQCAHPGKKLLFMGQELAQRHEWSEARSLDWHLLQHDSHRGI
                     QRLVADLNHLYAAEPALHQVEFDWHGFEWIDANDADNSVLSFIRRGKKPEDLIVAIFN
                     ATPVVRGGYRLGVPQSGFYFEIMNTDAAHYGGSNVGNMGGQQAADHPAQGRPHSLVLT
                     LPPLAAIFLKWQP"
     gene            6808..>8438
                     /gene="ggt"
                     /locus_tag="VGR03_08310"
     CDS             6808..>8438
                     /gene="ggt"
                     /locus_tag="VGR03_08310"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEV1994318.1"
                     /translation="MTGQVSEAQDRSQGRSMVISRNGIVAAESPLAAQAGARILERGG
                     NAVDAAIAANAMMGVVEPMMNGIGGDLFAIVYDVKANKLYGLNASGWAPKGLTIEYLQ
                     KQGIRSMPQQGVNAITVPGAVDGWQKLADKFGRKKLEEDLAAAIRTAQDGFPVPEWTA
                     AYWAMEVDHLRTDETAAKTYLPGDRAPKLGEVFRNPELAWSLEQIAQHGKEAFYKGEI
                     AAKILESMKRHNGPMTAQDLAEYFSEFVEPISTTYRDWTIYELPPNVQGIAALEMLNI
                     METFPLGQKDWGFGSTKALHAMIEAKKLAYADLARYIGEPKKQKLPVATLLSKDWAAQ
                     RAKLIDADKANCDATAGEMRVGSDTTYLSVVDREGNMVSLIQSNYEAFGSGIVAAGTG
                     FALHDRGGLFSMDPNSPNALAGRKRPLHTIIPAFAQKGDVRVAFGIMGGWNQSQAHAQ
                     FVANLVDFKMNIQAALEAPRFSKHTFSGCDVMMENRFGQNVRNELTSKGHKIEVKGTF
                     SSGMGGGQAVMRDFALGVNYGASDPRKDGQAVAELP"
BASE COUNT         1806 a         2019 c         2917 g         1696 t
ORIGIN      
        1 gttccggagt tcaactgtat ctgaaccgta cggtcatcca gattcaggaa ggaaaccgag
       61 gtctgcccgc ccacgcgaat cgccgttgac ccgatatgca tctcagctct cgagccattg
      121 cctgtccaga ggctatctcc cgcagtcatt ggacggttca gggtggccca gccccagtca
      181 ggctcaccgc ctggctggaa ggaaacacgg ccctccatgt aattcagccg ggccacacgt
      241 attggtggat catcaaacgc gaaggctggc cgggcaagca gaacgaacag acagagcacc
      301 aggcatacgc gcccgcagaa atttgcaaaa ccgaccgctg tcttcatcac tcagctcctt
      361 gcgggcacgc aattggcgcc ccattcttga gtaaccggca tctgaacgtg gctttcgtca
      421 aaggagttct cggaaacgtt aatcaggcaa cccaacttag accaagccct ggatgctggt
      481 ctccctcaaa atattcaacg ccctaagtat tagggtaaaa ctgaaaccga aagcttactg
      541 ttctgttttg tacattgcat tctcgatgag cccctgacgc cttgccgtgc tgcggggagg
      601 tactattgca tgcgcttaga cgggttggta cattcgatgg cgttctaact tccagcctca
      661 atccctgagg acgtagcgag taccctgcgt gagcagccgc cggtcgtttt ctgacacggc
      721 ggtgggctga cggtgcgcat tacacggggg atctcgcgga gcgtcgtttc tgaaaggaga
      781 cctcgatgat agtaaaattc tcacttgggc tttgttgctc cttggccatg ttgctcgtct
      841 tacttttgcc ccctccggtg atcggcgggg acataccaca gctagtggtg gacgatgaca
      901 aggtggaatg tccgaatgcc gcattcagcc ggattcaaga tgccgtggat gcggcgtcgc
      961 ctggcgcgac gattcgcgtt tgcaaaggga attaccacga gcaggtggcg atccacaaac
     1021 ccttgaccat cgatgcggac agcggcgcgg ttctgatgcc cggcacgatg cagcagaaca
     1081 cgacgagttt gctcgacggt gcacccatcg ctacagcgat tcttgtggcc gacacgacgg
     1141 acgtttccat cgagggcctg atcgtggacg gcgcgaacaa cggcgtgccg cagtgctcgc
     1201 cccgactctt cggaatcgct ttccaaaatg catcgggcga gatcagccaa gtggccattc
     1261 gtaacttcaa gctaggcacg ggtctgggtg gctgccagag cggcacgggg atttttgtcc
     1321 agagcggaag gggcggcatc tccaacgtcg aaattgaaaa gtccaccatc cacgatttcc
     1381 agaaaaacgg aatcaccgcc aatgaggtgg gaactcacgt gtcggtacac ggcaacgtcg
     1441 tcacgggcat cgggccgact tcgggagcgg ctcagaacgg catccagatc ggctttggcg
     1501 cagaaggcgc gatttcgagg aacacggtga cgaacaattt gtggtcgcca tgcacggccg
     1561 tggacacatg ccaggccgta gcgacgaaca ttttggtcac gcagtccgat ggagtggagg
     1621 tgactgacaa cgacgtgggg atcagtcagg tggggatttt ccttcacggg aaccaggggc
     1681 acgtggcgcg gaacgaaacg tttgcgagca gcgtcttcga cgggattcgc ctcgaaggcg
     1741 atgggaaccg ggtgcggcac aatcaggttt tcaacggcgc ggaatccggc atttttgtcg
     1801 ccggcaataa caacgcggtg gagcacaaca aaattacgga ggcagccgtc ggcattctca
     1861 aagagactgg ctctctcggc aatctgattc ggttcaacca ggtgttcgac acgctggttg
     1921 cggtgcagga ccctgccttg cctgggctag cgggcgtcgt ttcaccggag cggtagggac
     1981 ggttggagaa ttgacaggaa gcaacgacca acgtcatcgg tgtaaacaac atccgcgctg
     2041 acaaccaagg atgaggctgg agcttaagcg aacaggcggg atggattatg attggtgccg
     2101 tttttggaaa gaaaatagaa atcagtaaag tgaaactaga aaatggggag gggaaaatct
     2161 ccacacaccc gaccaggtcg gcgtaaacgc caaacgaggg tggcgcaccc gcggcgaaaa
     2221 taggaacagg gaaaagaaag atgccgacag ggatgccggc gctacaaagg cagcaggcaa
     2281 atcggcgcgg gttggcgcaa attgggacag cccgggtcag acgtcgcaga ggtcggcggc
     2341 ttggcgcaag gaggttagcg ggtcacgcgt ggggacgaat tcgtgggcga tgtagccgcg
     2401 gaagttggtg gcggcgatgc cgcgcatgac gccgtcccat tggacttcct gggtgccgtc
     2461 gagttcgtgg cggccgggca cgccgccggt gtggaagtgg ccgatccaat cgatattttt
     2521 ctggatggtg cgaaccaggt ctccttccat gatctgcatg tgatagatgt cgtagagcag
     2581 cttgacgcgg ggcgagttga cggcttggac gacctgcaca ccccaggggg tgtggtcgca
     2641 catgtagtct ttgtggtcga ccttgctgtt gaggagctcg atgcagatgg tgacgccgtg
     2701 gtcctcgccg attttcttga cgcggttgag gccgatgatg gtgttttcgg cgccctcttc
     2761 gtcggacatg ccagcgcggt tgccggagaa agtgatgacg ttggccacac cggctttggc
     2821 ggcgagcgga atgtacttgc gaaaggcttc ttcaattttg gcgtggtttt cgacgcggtt
     2881 catggcgctt gcgatatcgc caccgaaggc gtatcccatt gcgcagacca ggccatagcg
     2941 cccgggcact tcgaattcgt cggggttcag caggtcgacg gccttcaaac cgatttcggc
     3001 ggcgtaggca cagagcttgt cgagcggaat cttctggtaa caccagcggg aaacggactg
     3061 gcggatattg cccttgcgtt ttacgggagc gcttccctga gaccaggtga cggatgggag
     3121 agaagccatg gtggctcctg agagaacagt cttgagcacg ttgcggcgcg atagtttgga
     3181 catggtggac acgcagagtg taccgaagaa aagcctgatg cgggaggaga aattattttt
     3241 ttggaggctt ggggctttgc ggaggcgagg ccgcggcggt tttggggagt tcgacgtgcc
     3301 agatgtcttt ttcggattgc aggccgaaga cgccgacctg attggattcg gaggtgccga
     3361 cctggccgat ccagacgaca cggaaacggg ctttgcgcca gcggcggcgg acttcgatag
     3421 tttcaccggg cgatgtcaag aatccgacgc cgtccaaacg cgcgccatcc tggctgagat
     3481 cgacggtgta ggcgctctgg gcaaaaggat ggcccttggc gtccgtacct ttaacgatga
     3541 cacggagagt ttttgggatg cgaggcttct tgcggctttt cttccagaag tcggagatgg
     3601 gctgcttgaa ggctaggccg atgcgagttt cgcggtcgga gtaggcgtcg agatagacga
     3661 cgcggacttc ctgctcctgg cggctgctct tgtggatgag aaaaacggtg tcgcccaatg
     3721 cgagtttggt tttcagggtg acgagggcgc cgctggaatt gacgatgacc gtctcggtgt
     3781 cctcgtggaa ggcaccgcct ttgccagcaa aaccataaat cacgacgggt acgcgcatag
     3841 ggatgcgctc gctggagcgg agtactttct ttgcaggcgc ggtcgggtgg gggacggcaa
     3901 aagtcggcgg ctttttttgc gagggccgag gatcgtcgag gggcgctacc tccattgctt
     3961 cctggggaat tccctgctgg aggccgtgct cgcggcactt gaagtcccaa gtctgctgga
     4021 ggatctgctc gagggattcc tcgcggttcg aaagggtgac ccaagagtcg ctgtccccgt
     4081 gagggcacct gagccggatg gcgaagttgc taggcatgtg agttccgtac gcattttgat
     4141 gatacctgtt tggcgcgagt ctggcggcgg actacccggt ggctttcctc gaaaagtaaa
     4201 cgggtgaggg ttgggaagca aatggcaggc ctaaggttgc cacttcagga aaatggcggc
     4261 gaggggcgga agagtgagaa cgagggaatg cgggcggccc tgggcggggt gatcggcggc
     4321 ttgctggccg cccatgttgc cgacgtttga gccgccgtag tgggcggcgt cggtgttcat
     4381 gatttcgaag tagaagccgg attgtggaac gccgagacga taaccgccgc ggacgacggg
     4441 agttgcgttg aagatggcga cgatgaggtc ttcgggtttt ttgccgcggc ggatgaagga
     4501 aagaacactg ttgtcggcgt cgttggcgtc gatccattcg aaaccgtgcc agtcaaactc
     4561 gacctgatgg agggcgggct cggcggcata gagatggttg agatcggcga cgaggcgctg
     4621 gataccgcgg tgggaatcat gctggaggag gtgccaatcg aggctgcgag cttcgctcca
     4681 ttcgtggcgc tgggcgagct cctggcccat gaagaggagt tttttgcccg ggtgggcgca
     4741 ttgataggcg tagagcagac ggaggttggc gaattgctgc cacatgtcgc cgggcatctt
     4801 gtgaagaagc gagtttttac cgtggacgac ttcatcgtgt gagaagggaa gcaagaagtt
     4861 ttccgtgaag gcgtagagga gcgagaaggt gagtttgttg tgctcgtatt ttcgatgaac
     4921 gggatcgtgg gaaaaatatt ttagagtgtc gttcatccag cccatgttcc atttgaaact
     4981 gaagccgagg ccgccgagat aagtggggcg ggagacggcg ggccaggaag tggattcttc
     5041 ggcgatggtg agaactccgg ggaatttgcc atgggcgact tcgttcatgc gcttgaggaa
     5101 gtcgatggcg tggagatttt cgcggccgcc gaattgattg ggaacccatt cgccgggctt
     5161 gcgcgagtag tcgagataga gcatggaggc gacagcgtct acgcgcaggc cgtcgatgtg
     5221 gtacttgtcg agccagaaga gagcgttgga gatgaggtag ttctggactt cgttgcggcc
     5281 gtaattgtag acgagcgtac cccagtcggg atgggcgcct tggcgtgggt cggagtgttc
     5341 gtagagatga gtgccgtcga attgggagag gccgtgggtg tcgcggggga aatgcgcggg
     5401 agtccaatcg agtaggacgc cgaggccggc ctggtgacag cggtcgacaa attccatgaa
     5461 ttcggcggga gtgccgtaac ggctggtggc ggcgaagtag ccaagcgtct ggtagcccca
     5521 ggagccgtcg aagggatgct ccatgatggg gagaagttcg atgtgtgtgt agccgagatc
     5581 cttgacgtag gggatgagct ggtcgccgag ctcgcggtag gtgagccagc gatcgtgatc
     5641 gtccgggact cggcgccatg agccgatatg gacttcgtag atggagatcg gtgattcgaa
     5701 ccagttcttc tgggcgcggt gggagatcca gtcggcgtcg gtccattcat ggtgatcgag
     5761 acgcgcgacg acggagccgg tgttgggacg cagttcggag cggaaggcgt agggatcggc
     5821 tttgagtggg agaatgttgc cgctggggcc gacgacttca tatttgtaga tggcgccttc
     5881 gggaagctcg gggacgaaga tttcccagag accggaggag ccgcgggcgc gcatgggatg
     5941 cacgcgaccg tcccaacgat tgaagtcgcc cacaacgctg acgcgttttg cgctgggggc
     6001 ccaaacggcg aagtgaacgc cgcggacgcc ttcgagtgtg atgacgtggg cgccgagttt
     6061 ttcataagtg tcgtagtggc ggccctcacc catcaggtac agatcgaatt cggtgagcac
     6121 aacggggaaa gcgtaggtat cgaagatttc gaaggagtcg ccgaagtgag tgcgcccatg
     6181 aatcttgtaa ctgcctgggg aaggcggacc ggattggttt gaaggccagg tggcttcgaa
     6241 gaagccttcg gggcgaagtt tcatggcgtc ggtgacttta gcaggggaaa gcgtagcgcc
     6301 ttcaagggag ggaggcttca aagagatgca ggcttccgcg gcccagggca ggaaaaagcg
     6361 caccgcccag ttgccgtcaa cgggatgcgg gccgaggagg gcaaagggat cggaatggcg
     6421 cgcggcgagg agagcgtcga tctgtgcgtc gaggcgcgga tcgcggggag caatcggtgc
     6481 cgtgtgtttc atggctgggc gctgctcaga caagtcggtg gtctccgcaa agtagcaaat
     6541 gagggatcag cagacaagct tagctggaac gctgacccac gtcctctgaa ttctagggcg
     6601 ttggcgggaa aatgccaatc aatccgtgat gggggaaggg gacggagatg cttgcgggtg
     6661 cccatggtac gagttagagt gcgagagcac gcgacttctg tgcggatgcg gagtttaaac
     6721 gaactaacga aggagcggac caagtcgatg cggattttcc ccagattgct ggttttccta
     6781 tttgttctta gaggagcggc aatactaatg actggtcaag tgagcgaagc gcaggaccgc
     6841 tcgcaggggc ggtcgatggt gatctcgcgc aatgggattg tggcggcaga atcgccgctg
     6901 gcggcgcaag cgggggcgag aattctggaa cgcggtggga acgccgtgga tgcggcgatc
     6961 gcggcgaacg cgatgatggg cgttgttgag ccgatgatga atgggatcgg cggggatttg
     7021 tttgcgatcg tgtatgacgt gaaggcgaac aagctctacg gattgaacgc gagcggatgg
     7081 gcgccaaaag gattgacgat cgagtatttg cagaagcagg gaattcggag catgccgcaa
     7141 cagggagtga atgcgattac ggtgccgggc gcggtggatg gctggcagaa actcgcggac
     7201 aaatttggaa gaaagaaact ggaggaggat ctggcggcag cgattcgaac ggcgcaagac
     7261 ggttttcccg ttccggaatg gacggcggca tattgggcga tggaggtgga tcacctgcga
     7321 accgatgaga cggcggcgaa gacctatctt ccgggagacc gcgcaccgaa attgggtgag
     7381 gtgtttcgca atcccgagct ggcatggtcc cttgaacaga ttgcacagca cggcaaggag
     7441 gcgttctaca aaggcgaaat tgccgcgaaa attctagagt cgatgaagcg gcacaacggg
     7501 ccgatgaccg cgcaagacct ggccgaatac ttcagcgaat ttgtcgagcc gatttccacg
     7561 acgtatcgcg attggacgat ctacgagctg ccgcccaacg tgcagggcat cgcggcgctg
     7621 gaaatgctga acattatgga gacgtttccg ctggggcaga aggattgggg ctttggctcg
     7681 acgaaagcac tgcacgccat gatcgaggca aagaagctgg cgtatgcgga tcttgcgaga
     7741 tatatcggcg agccgaagaa acagaaattg cctgtggcga cgctgctttc gaaggattgg
     7801 gcggcgcaaa gggccaagtt gattgacgca gacaaggcga actgcgatgc aacggctggt
     7861 gaaatgcggg ttgggagcga cacgacgtat ctgagcgtcg tcgaccgcga gggaaacatg
     7921 gtgtcgctca tccagagtaa ctacgaggct tttggatcgg gcattgttgc tgccggcacg
     7981 ggatttgcgt tgcacgatcg cggcggactc ttcagcatgg atccaaattc accaaatgcg
     8041 ctggcgggga gaaaaaggcc gttgcatacg atcattccgg cgtttgcgca aaagggagat
     8101 gtgcgggtgg cgtttgggat catgggcggc tggaatcagt cgcaggcgca cgcgcaattc
     8161 gttgcgaatc tggtggactt caagatgaat attcaagcgg cgctggaggc accgcggttc
     8221 agcaagcata cgttcagcgg ttgcgacgtg atgatggaaa accgttttgg gcagaatgtg
     8281 cggaatgagt tgacgagcaa aggccacaag attgaagtga aagggacatt ttccagcggg
     8341 atgggcgggg ggcaggcagt catgagggat tttgcgctgg gagtgaatta cggggcttcg
     8401 gacccccgaa aggatggtca ggcggtggcg gaattgcc
//
LOCUS       DASYVO010000249        11091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_163459, whole genome shotgun sequence.
ACCESSION   DASYVO010000249 DASYVO010000000
VERSION     DASYVO010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 11091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11091
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163459"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VGR03_08315"
     CDS             <1..774
                     /locus_tag="VGR03_08315"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1994319.1"
                     /translation="GFPDGIMGPELIENMRKQAQKFGAEFKAGAVSEVDVSKRPFKIT
                     AGKDTYETKALIVAAGASARLLGLKGEKELIGHGVSTCATCDGYFFRGKPIAVVGGGD
                     SAMEEANFLSRYASEVHLIHRRNEFRASKIMIDRVKANAKVQFVTPFAIEEILAPQGF
                     VGGVRLRNLETKEAREIPLDGVFVAIGHDPNTTVFKGKLEMDANGYLLAKHGSLTAIP
                     GVFVAGDVQDHRYRQAVTAAGSGCMAAIDAEKFLEEHKD"
     gene            810..1721
                     /locus_tag="VGR03_08320"
     CDS             810..1721
                     /locus_tag="VGR03_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEV1994320.1"
                     /translation="MLRSTLLKLSESKGFANWVTTNGTTRRMARRFVAGETLDEAIAA
                     ARDCNNAGMLASLDYLGENVATTSDAQHSRDAYLEVFERIAQEGLHANVSGKLTQLGL
                     DINIEFCEGLTLSIVERAAGFDNFLRIDMEGSLYTQRTIDLVKRVRTRNPAVGTVIQS
                     YLYRSETDVADLLAYGCRIRLCKGAYKESEDVAFPRKADVDANFVRLMQMLLSSGFYH
                     GIATHDPRMIGATIRHAAAKQISKDDFEFQMLYGVRTDLQRRLVKDGYRVRVYIPYGR
                     DWFPYFMRRLAERPANLGFLVRNFFRS"
     gene            complement(1735..2391)
                     /locus_tag="VGR03_08325"
     CDS             complement(1735..2391)
                     /locus_tag="VGR03_08325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV1994321.1"
                     /translation="MIRSENLRSGYIQEDVEEYLYQLLPERDTVVTEIEDYAAQNRIP
                     IIGPAVARMLALFVQVSGAKRIFEMGSAIGYSTIWLARAAGPKAKVIYTDGDPEKARR
                     AKDYFRRAGVAKRIEVRVGNAHDLLQKTSGKFDLIFNDVDKHQYPAALRVALPKLRRG
                     GLFITDNTLWSGKAARPAAPDDADTLGVQELNRLVYASKDLYPVLIPLRDGVTVCRKR
                     "
     gene            complement(2450..2974)
                     /locus_tag="VGR03_08330"
     CDS             complement(2450..2974)
                     /locus_tag="VGR03_08330"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV1994322.1"
                     /translation="MRIRTCLVSLALLGAATAMAAPQASKTAAPRDPEIIDAQGYQKL
                     LERYRGKPLLVNFWATWCEPCRDEYPMLNELAKQYAPQGLKVVGVSMDDDGDLILMRR
                     FLARYKPVFPNYRKKAGGEELFRQGVLPGWNGVLPASVMYARDGRLIGHVFGEGTRDS
                     YEAAIRTLLNSGSN"
     gene            3157..3426
                     /locus_tag="VGR03_08335"
     CDS             3157..3426
                     /locus_tag="VGR03_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEV1994323.1"
                     /translation="MAYVIAEPCIGTKDTACVDVCPVDCIHPRKDEANFEGETQLYIH
                     PTECIDCGACVPVCPVTAIFALEDLPEKWNSFTPINADWYSKQGK"
     gene            3642..5417
                     /locus_tag="VGR03_08340"
     CDS             3642..5417
                     /locus_tag="VGR03_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV1994324.1"
                     /translation="MKPGNLCLALAISFFSLAVSSTAWAQTPAVHRPQPRRGSSTLEG
                     HWTGSLQAGEAVLHLVLHVSKAEDGSFRANIDSLDQGVYGIEVTALTQKDSTLHFNVA
                     SVGASYEGKFAADHGSIEGEWTQGSASLPLVFHRQAAGAGAKKPSDAIASAEGVWQGA
                     LEGNGMRLRLQLHVSHDDQKQLVAALDSPDQGVSGLPAIKVSQKDAGFHFEILVVSGV
                     YDGTVNAAKTAITGSWTQNGVEQKLDFRRSDQLLELVRPQNPVKPYPYKEEEITFPNE
                     KAKISLAGTLTLPRGQGPFPAAILLSDSGPRDRDESLVGHRPFLVLADHLTRKGIAVL
                     RFDKRGNGKSTGDYANAATEDFAGDAEAALAYLKTRKEIDVKKIGLIGHSEGGTIAPL
                     VAAQSGDVAWIVLLAGPGLKGEDILLLQSELILKTAGVNDDQIAKTREFNKQTYALVR
                     HEKDPAALQAKLSGLVQSSGMSASLPPAAVESQVRLMVSPWFRFFLDYDPLPALQKTM
                     CPVLALNGEKDLQVAPKENLAKIQKALQDGGNKDFQTTELPDLNHLFQHCPTGSPTEY
                     GGIQETIAPEALNAVSDWVLKHTAP"
     gene            5552..7690
                     /locus_tag="VGR03_08345"
     CDS             5552..7690
                     /locus_tag="VGR03_08345"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HEV1994325.1"
                     /translation="MPQKFHLALLIHAHQPCGNFEHVLERAYDTSYLPFIEHLKAHPG
                     VRLGLHYSGPLLTWIEEHRPEYFVRLKKLVEGFQVELVGGGFYEPILVSIPPEDQREQ
                     ITRLAVYLEKHFGRLPSGAWLAERVWEPQLPTALAAANVAYTLVDDIHFLSAGFEPEE
                     LFGAYIAEDRGKTVCLYPGQKALRYLIPFGKVEDVIAYLRDAASVHPGGVAAMGDDME
                     KFGVWPGTHEHCYKDGWLSDFFTALEKNSDWLAVSTPGGYLESHAPLGRADLPTASYT
                     EMMEWALPTRVRQRYHEVLKEFSARPEILSFLRGGSWRGFFRKYPESNLLHKKMLRVS
                     ARIAAAPVRHDGSKSSDELLQARDLLLRAQCNDAYWHGIFGGIYAPHLRTDPWRNLIR
                     AEAIADRQTPGALVARVELLDYDADGANELLFTSPEAQALLKPNDGGTIAALDFRPAA
                     ATLVNSILRRPEAYHTRLREAAGKPATGAVASIHEQTRVKEPGLERFLRYDRWPRHAF
                     RVLIFDPARTHADYEALELREDAGFAGGAFTVKSSAPYEAELFRADSLTLLGKNESAT
                     AKLLLLKQYSFSPAPNGFEAACEITLKFKETLERPVAVGIESVINLLAPTEPDRFFET
                     PTGRHNLRFSGVLPGPILRMEDGWQRVRIALHAPLVEEFWVAPIETVSESEEGFERVY
                     QGSQILALWRPPLTTQKTWSTRLVWRIESF"
     gene            7725..8315
                     /locus_tag="VGR03_08350"
     CDS             7725..8315
                     /locus_tag="VGR03_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994326.1"
                     /translation="MNSRLMFIGVLLLTTASSSPQETQSTSGDCRIITRADLADPNAP
                     VFEAYRVTNRQGVSKPKLDLTSNPIAKTYRTVLRRDIVEGPNYAGHYRVVVWGCGSSC
                     AMFAVVNLKTGRVITPKSFSAVSEVHVLADDFLQDTESKSWVVRFKRNSKLLVAIGAL
                     DEDDDREGAFYFLLEGESLRLIHSTVVKKNCDNTNH"
     gene            complement(8496..8894)
                     /locus_tag="VGR03_08355"
     CDS             complement(8496..8894)
                     /locus_tag="VGR03_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEV1994327.1"
                     /translation="MSAVLDTHTVLWYLEKSQELSALARTTIEEAIREARDVYVSAIS
                     LVETVYLVERRRLPLTALERLRSALSESNSGLFVASVDAGVADALQNIPRDHVPDMPD
                     RIIAATALHLGLPLVTRDRRLQAAGIQTIW"
     gene            complement(8891..9118)
                     /locus_tag="VGR03_08360"
     CDS             complement(8891..9118)
                     /locus_tag="VGR03_08360"
                     /inference="COORDINATES: protein motif:HMM:NF021558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HEV1994328.1"
                     /translation="MTIEQQVLEKLRDLPPEKQKEVLDFVDALKEKNGPKKPRRSLLG
                     LWADLNIHITEEDIAQARREMWGNFPRDIKL"
     gene            complement(9811..10785)
                     /gene="egtD"
                     /locus_tag="VGR03_08365"
     CDS             complement(9811..10785)
                     /gene="egtD"
                     /locus_tag="VGR03_08365"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEV1994329.1"
                     /translation="MATPALSLSLRHVSEFASEVRESLTKPGQRELPSKYLYDEVGSA
                     LFETICVLPEYGLTRADARLLEKYAGEIVGRLPSPIQVAELGSGSGKKTRWILEALAR
                     RQMTYYYPIEISPSALAACAKELGQIDLVSVVGYEQPYLEGLRAVADGRGERDHLLVL
                     FLGSTIGNFDRYAGETFLSEMREILQPGDALLLGTDLEKSVELQLLAYDDPAGVTAAF
                     NLNLLARINRELGADFDLSCFRHEARWNRVERRIEMHLHSLRRQTVHIPAASLRLMLD
                     EDETIWTESSHKYQAEEIIEMAARTGFRCEGQWIDREWPFAQNLLIAG"
     gene            complement(10810..>11091)
                     /locus_tag="VGR03_08370"
     CDS             complement(10810..>11091)
                     /locus_tag="VGR03_08370"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEV1994330.1"
                     /translation="SPVGAHPAGASAFGVHDLVGNGWEWTRTEFAPFPGFTAMPFYSN
                     YSANFFDGKHYVMKGGSPRTAACMLRRSFRNWFQPHYPYVYATFRCVED"
BASE COUNT         2302 a         3394 c         2976 g         2419 t
ORIGIN      
        1 ggcttcccgg acggcatcat ggggccggaa ctgatcgaga acatgcgcaa gcaagcgcaa
       61 aagttcggcg cggagttcaa ggccggagcg gtcagcgaag tcgacgtcag caagcgcccg
      121 ttcaaaatca ctgccggaaa agatacgtat gaaacaaaag cgctgatcgt cgccgcggga
      181 gcttccgcgc gcctgctggg cctgaaaggc gaaaaagagc tgatcggcca cggcgtctct
      241 acctgtgcca cctgtgacgg ttatttcttc cgcggcaagc ccattgcagt ggtcggcggc
      301 ggcgattccg cgatggaaga agccaatttc ctctcgcgct acgccagcga ggtccatctc
      361 atacaccggc gaaacgaatt tcgcgcctcc aagatcatga ttgaccgcgt caaggccaat
      421 gccaaagtcc agtttgtcac gcccttcgcg attgaggaaa ttctcgcccc ccagggtttt
      481 gtcggaggag ttcggctgcg caatctagag accaaggagg cgcgcgaaat tcctctcgac
      541 ggtgtattcg tggccatcgg tcacgatccg aacaccaccg tcttcaaggg caaactcgag
      601 atggatgcga acggctacct gctcgctaag cacggcagcc tcacagcgat tcccggcgtg
      661 tttgttgccg gcgacgtgca agaccatcgc taccgccagg ccgtcacggc tgcgggttcc
      721 ggttgcatgg ccgccatcga tgcggaaaag tttctggaag agcacaaaga ctagtttcgt
      781 acttcccagg ctgccataat tggtcccgca tgctccggtc gacgctgctc aagctttccg
      841 aaagcaaagg cttcgccaat tgggtcacta ccaatggcac cacgcgccgc atggcgcgcc
      901 gcttcgtcgc aggcgagacg ctggacgaag ccatcgccgc tgcccgcgat tgcaataacg
      961 caggcatgct cgccagcctc gattatctcg gcgaaaatgt cgccaccacc tcggatgcgc
     1021 aacattcccg cgatgcctat ctggaggttt ttgaacggat cgctcaggaa gggctgcacg
     1081 ccaacgtttc cggcaagctc acgcaactcg gcctcgatat caacattgaa ttttgcgaag
     1141 gcttgactct ctccatcgtg gagcgcgccg ccggcttcga caattttctg cgcatcgaca
     1201 tggaagggtc tctgtatacg cagcgcacca tcgatctcgt caaacgcgtt cgcacgcgca
     1261 atcccgccgt cggcacggtc atccagtcct atctttaccg cagtgaaacc gacgtcgcgg
     1321 atctgttggc gtatggctgc cgcatccgtc tgtgcaaggg tgcgtacaag gaatcagagg
     1381 acgtggcgtt tccgcggaaa gccgatgtcg atgccaactt cgttcgccta atgcagatgc
     1441 tcttgtccag cggcttttac cacggcattg ccacacacga tccgcgcatg atcggcgcga
     1501 ccattcgcca tgcggcggcg aaacagattt ccaaggatga tttcgaattt cagatgttgt
     1561 acggagtgcg caccgacctg cagcgccggc tcgtcaagga tggctatcgc gtccgcgtct
     1621 acatccctta tggccgcgac tggttcccct acttcatgcg gcgtctcgcc gagcgtcccg
     1681 caaacctcgg tttcctcgtc cgcaatttct tccgttcgta agcttatttc cgcgctatcg
     1741 cttccggcag acggtgacgc cgtcacgcag cgggatcaat acggggtaga gatctttcga
     1801 agcgtagacc aagcggttga gttcttgcac gccaagtgtg tcggcgtcgt ccggtgccgc
     1861 cggccgcgcc gcctttcccg accacagcgt attgtcggtg atgaacaagc cgccgcggcg
     1921 cagtttcggg agtgccacgc gtaacgcggc agggtattgg tgcttatcga cgtcattgaa
     1981 aatcagatcg aacttgcccg acgttttctg cagcaggtca tgcgcgttgc caacccgcac
     2041 ctcgatccgt ttggcgacgc ctgccctgcg aaaataatct ttcgcgcggc gtgccttctc
     2101 cggatcgcca tccgtgtata tcacttttgc cttcggcccg gccgcgcgcg ccagccagat
     2161 cgtggaatac ccgatggccg atcccatctc aaaaatccgc tttgcgcccg aaacttgcac
     2221 gaacagcgcc agcattcgcg ccaccgccgg cccgatgatc ggaatgcggt tctgcgcggc
     2281 gtagtcctcg atctcggtga cgaccgtatc gcgctccggc agcagttggt aaagatattc
     2341 ctcgacgtcc tcctgaatgt agccgctgcg tagattctcg ctgcgaatca tagttcctct
     2401 cccggcttca gtgcgtgaaa aacataggtt tcgatggtgc ggagccgcat tagttggatc
     2461 ccgaattgag cagcgttcga atcgccgcct cataactgtc ccgcgtgccc tcgccgaaca
     2521 cgtgcccaat cagccgtccg tctcttgcgt acatgaccga agccggaaga accccgttcc
     2581 aacccggcaa taccccttgc cggaacagtt cttccccgcc cgcctttttc cggtaattgg
     2641 gaaataccgg cttgtagcgg gccaggaagc gccgcatcag aatcaaatct ccatcatcat
     2701 ccatgctcac acccacgact ttcaatccct gcggcgcgta ttgcttcgcc aattcattca
     2761 gcatggggta ctcgtcgcgg cacggttcgc accacgtcgc ccagaagttc accagcagcg
     2821 gttttccgcg gtatcgctcg agcaatttct gatagccctg tgcgtcgatt atctccgggt
     2881 cgcgcggagc cgcggtcttc gaagcctgcg gtgccgccat tgcggtcgcg gcgcccagta
     2941 aagcaagcga tacaagacaa gtccggattc tcatgctcgt tccttctcct cccattctat
     3001 ccgtaccccc atcggttgag aaacttcttc ccaccgcgta caattcccat agctgcttgt
     3061 cgctccgggc agtcgcgtgg tataagcaat ttttgccgct ggtgctgttt cgttggatgg
     3121 agcgccggtc ggctgtccga acaaaggaga cccttaatgg cttacgtgat tgccgagccg
     3181 tgcatcggca cgaaagacac cgcctgtgtg gatgtctgcc cggtggactg catccatccg
     3241 cgcaaggatg aagcgaattt cgagggcgag acgcaactgt atatccatcc cactgaatgc
     3301 attgactgcg gagcgtgcgt cccggtctgc ccggtgacgg ccatctttgc gctcgaggat
     3361 ctgccggaaa agtggaatag ctttacgccg atcaacgccg attggtattc aaaacaggga
     3421 aagtagcttg gtctgttagg acgcaactgg tttttcgcaa ttttgagcgg cacgctccgt
     3481 cgtgccgttt ttttctatct cggcgctcga gaattcgcgc ctaatctttg agaattgatt
     3541 tgcgccactc ctaatccgcg ccgttctgca ccagttacca tgccaactct tttgagccgg
     3601 gaatcatcta ccatcctgaa tgtggtgtgt ggaggtcttg cgtgaaaccc ggaaacttgt
     3661 gtctggcctt ggcgatttct tttttcagcc tcgctgtttc ttcgacggcc tgggcccaga
     3721 ctcccgccgt tcatagaccg cagccgcgac ggggctcctc caccctggaa ggccactgga
     3781 ccggctcgct tcaagcgggc gaagcggtcc tccatctcgt tctgcacgtt tcaaaagcgg
     3841 aggacggctc cttcagagcc aacatcgaca gcttggatca aggcgtctac ggcattgaag
     3901 tgaccgctct cacgcagaaa gactccactc ttcatttcaa cgtggcttcc gtgggcgctt
     3961 cctatgaagg aaagtttgcc gcggatcatg gcagcatcga aggtgaatgg acgcagggca
     4021 gcgcaagcct gccgctcgtt tttcaccgcc aagcggcggg agccggcgca aagaagcctt
     4081 ccgatgccat cgcgagcgcc gagggagtgt ggcaaggcgc gctggaaggc aacggcatgc
     4141 gcctgcgctt gcaattgcac gtctcgcatg acgatcagaa gcagctcgtc gccgcgctcg
     4201 atagtcccga ccagggagtg agtggcctgc ccgccatcaa ggtgagtcag aaggacgccg
     4261 gctttcattt tgaaattctc gtggtgagcg gcgtttatga tggtacggtc aacgccgcaa
     4321 agacggctat caccggaagc tggacgcaaa acggcgtcga gcagaaactg gattttcgac
     4381 gttcggacca acttcttgaa ctggtccgcc cgcaaaaccc tgtaaaacct tatccgtata
     4441 aggaagaaga aatcaccttt ccgaacgaga aagcgaagat ctcactggcc ggcacactca
     4501 ctcttccgcg cgggcagggt ccgtttcccg ccgccattct cctcagtgat tccgggccgc
     4561 gcgaccgcga cgaatctctc gttggacatc gcccttttct cgtgttggcg gaccacctga
     4621 cgcgaaaagg catcgccgtt cttcgtttcg ataagcgcgg aaacggcaag tccaccggcg
     4681 attatgcgaa cgcggcgacg gaggattttg ccggcgacgc cgaagcggcc ctcgcttact
     4741 tgaaaactcg taaggaaatc gatgtgaaga aaatcggcct catcggacac agcgaaggcg
     4801 gcacgatcgc gcccctggtt gccgcgcaat ccggtgatgt tgcctggatc gttcttctcg
     4861 cggggcccgg tctcaagggc gaggacattc ttctgctcca gtccgagctt atcctgaaga
     4921 cggcgggcgt gaacgatgac cagatcgcaa aaacgcgtga attcaacaaa caaacttacg
     4981 cgctagttcg ccatgagaag gacccggcag ctctgcaagc aaagttgagc ggcctcgtgc
     5041 agtccagcgg catgagcgcg tcgctccctc cggcggcggt ggaatcgcag gtgcgcctaa
     5101 tggtctcgcc gtggttccgg ttcttcctcg actatgaccc cctccccgca ttgcaaaaga
     5161 cgatgtgtcc ggtcctcgcc ctcaatggtg agaaggatct tcaagtcgcg ccgaaggaga
     5221 atctcgctaa aatccagaaa gccttgcaag acggcggcaa caaggacttt caaactaccg
     5281 aattgcctga cttgaaccac ctcttccagc actgtccaac gggctcgcct accgaatacg
     5341 gcggaattca ggagaccata gccccggagg ctctgaacgc cgtctccgat tgggtgctaa
     5401 agcacactgc gccctgatcg agccacgcag cagcgcccat actggctcgc cccgcatcca
     5461 aaagaggtgc gcaattcgcg cattccctac gcgagcggaa ttcacaattt cgcctggcgg
     5521 gttatgatgt tagagagaaa aagctgtccc catgccgcag aaattccatc tcgccctgct
     5581 cattcacgcg catcagccgt gcggcaattt cgagcatgtc ctcgaaaggg cttacgacac
     5641 atcttatctt cccttcattg agcacctcaa agcgcatccg ggagttcgtc tcggcctcca
     5701 ctattccggg ccgctgctca cctggatcga ggaacatcgg cctgagtatt ttgttcgctt
     5761 gaaaaagctg gtcgaaggct tccaggtgga actcgttggt ggcggcttct atgaaccgat
     5821 cctggtttcg attcctcccg aagatcagcg cgaacagatc acccgcctgg ccgtctatct
     5881 cgagaaacac tttggacggc ttccgtccgg cgcctggctc gccgagcgcg tctgggaacc
     5941 gcagcttccc accgccctcg ctgcggcaaa cgtcgcttac acgctcgtcg atgacataca
     6001 ttttctttcc gcgggcttcg aaccggaaga actctttggc gcctacatcg ccgaagaccg
     6061 tggcaagacc gtgtgtcttt accccgggca gaaagctctg cgctatctca ttccttttgg
     6121 caaagtcgaa gatgtcatcg cttatctgcg cgatgctgcc agcgtccacc cgggcggcgt
     6181 ggctgccatg ggcgacgaca tggaaaaatt cggcgtttgg cccggcacgc acgaacactg
     6241 ctacaaggac ggctggctct ccgatttttt taccgcgctg gaaaagaatt cggattggct
     6301 ggcagtttcc acgcccggcg gctatctgga atcacacgcg cctctcggcc gcgccgatct
     6361 gcccaccgct tcctacacgg agatgatgga gtgggcgctc ccgacgcgcg tccggcagcg
     6421 ttatcacgaa gtgctgaagg agttcagcgc gcgcccggaa atcctgtcct tccttcgcgg
     6481 cggttcctgg cgcggctttt tccgcaaata cccggaatcc aacctgctgc acaagaaaat
     6541 gctgcgcgtt tccgcacgca tcgccgctgc gcccgtgcgt cacgatggct caaagtcctc
     6601 cgatgaattg ctgcaagcgc gtgatctgct cttgcgagcc cagtgcaacg acgcctattg
     6661 gcacggcatt tttggcggaa tttatgcccc gcatctccgc accgatccct ggcgcaatct
     6721 gattcgcgcc gaggccatcg ctgaccggca gactcccggc gcgctcgttg cgcgtgtcga
     6781 actcttggac tacgacgccg acggcgccaa cgagttgctg tttacctctc ccgaagctca
     6841 ggcgcttttg aaacccaatg acggcggcac tattgccgcg ctcgatttcc gccccgcggc
     6901 cgccacgctc gtcaattcca ttctgcgccg ccccgaggcc tatcacacgc gcttgcgcga
     6961 agccgcggga aagcccgcca ctggcgccgt cgcgagcatt cacgaacaga cccgagtgaa
     7021 agagcccggc ctggagcgct tccttcgtta cgatcgctgg ccgcgccacg ccttccgcgt
     7081 cctgatcttc gatcccgcgc gcacgcacgc cgactacgaa gctctcgaat tgcgcgaaga
     7141 cgccggattc gccggcggcg ctttcaccgt gaaaagctcc gcgccttacg aagccgagct
     7201 tttccgcgcg gattccctga cgctgctggg caagaatgag tctgcaactg ccaaactcct
     7261 gctcctcaag caatattcct tcagtcccgc ccccaacggc tttgaagccg cctgcgaaat
     7321 caccctgaag tttaaggaaa ctctcgagag gcccgtggcc gtcggcattg aatcggtcat
     7381 caatctcctt gcccccacgg agcccgatcg ctttttcgaa acgcccacag gccgccacaa
     7441 tcttcgcttc agcggcgtac tgcccggtcc catcctgcgc atggaagacg gctggcagcg
     7501 cgtccgtatc gcgctgcacg ccccgctcgt cgaagaattc tgggtcgccc ccatcgaaac
     7561 cgtctccgaa tccgaagaag gcttcgagcg cgtctaccag ggatcgcaaa ttctcgccct
     7621 ctggcgtccc ccgctcacca cccaaaaaac ctggtccacc cgcctcgtct ggcgcatcga
     7681 atcgttctga actgcgtaac actagcttta aggagcgggc aaacgtgaat tcaagattga
     7741 tgttcatcgg tgtcttgctt ctaaccacgg catcctctag tccgcaagaa actcaatcga
     7801 catcaggtga ttgccgcatc attacaagag cagatctggc tgaccccaac gctccggttt
     7861 tcgaagccta ccgagtaacg aaccggcaag gcgtctcgaa gccaaaactc gacttgacga
     7921 gcaatccaat tgcgaagaca tatcgaactg ttctgcgaag agacatagtc gagggtccaa
     7981 attatgccgg acattacagg gtagttgttt ggggctgtgg ttcctcttgc gcaatgttcg
     8041 cggtggtgaa tctgaagaca gggcgagtaa taactcctaa gagcttttct gccgtgagtg
     8101 aagtgcacgt attggccgac gattttctgc aagataccga gagtaaatcc tgggtagttc
     8161 gttttaagag gaacagtaaa ttgttggtcg cgattggtgc tctggatgag gatgacgaca
     8221 gggaaggggc cttctatttc ttacttgagg gcgaaagcct acgcctcatc cactcgactg
     8281 tcgtcaagaa aaactgcgat aacacgaatc actaagactg ctgttaaaca cttcgcaagg
     8341 tccattcttc atttcatcct gccactaaca aatcctgcga aggcccattc actgtcgatc
     8401 cattgtccat cacagcgaaa tcccgtacgc gacgctttgc aggaatgcga cctcgcacgt
     8461 gacatggtga gccttatcct catggcctat tggggtcacc agatcgtctg gattccagcg
     8521 gcttgaaggc ggcggtcgcg ggtcaccagc ggaagcccaa ggtggagggc agttgctgcg
     8581 atgatacggt cgggcatatc aggaacgtgg tcgcgaggaa tattctggag cgcgtcggct
     8641 acaccagcat ccacggatgc gacgaacaat cctgagttcg attcactgag ggccgatcgc
     8701 agccgctcta aagccgtaag tggaagcctt cgtctttcca ccagataaac cgtttcaacg
     8761 agggagatcg ccgatacgta cacatcgcga gcctcgcgga tagcttcttc gattgttgtc
     8821 cgggctagcg ctgacaattc ctgagacttt tccaaatacc aaaggacggt gtgggtgtcc
     8881 aacaccgcgc tcatagtttg atgtcacgcg ggaagttgcc ccacatttcg cggcgggcct
     8941 gggcgatgtc ctcttccgtg atgtggatgt tcaggtctgc ccacaaaccc agcaggctgc
     9001 ggcgcggctt cttcgggccg ttcttttcct tcagagcgtc cacaaagtcc aacacttcct
     9061 tctgcttctc aggaggcaga tcgcgcagtt tttcgaggac ctgttgttcg atggtcatgg
     9121 cagtctctca ggcgatccct tttgattata cgcctgctcg acgcgattcc gagccattcg
     9181 gcgcggagct gccaacatct tcaggattgc tggcagttgc ggcattcgat gcccctacgc
     9241 aaactgcaag agcgataatt atggaacacg aactctcaaa caccgtcaaa accatgaact
     9301 gcaaccagga agaagtaaga cgagagagct acttagtgtt ttttcacctg cttacaagga
     9361 tccggttgct cggaccgcaa agttttcctt gactatccgc tatcccatgc tataattgat
     9421 gttgtaggga acgttatttg gagtgcggca gcttgctgcc gcttttcttt tttcagggca
     9481 gccttgtttg cctacgttcg ccttgcacaa ccaaaaacat gtcctttgga atctgtcatt
     9541 tgtgaaatgc aaatttcgta agtcttttct tttgatattc atgcaaatgg gtggggggtg
     9601 taaggggtgg cgccgacctt acaattcgac taagctccct agaatccggg cttccgcaaa
     9661 atgcctccat tttgcacaat tttggtgcat tttaactccc tttagaatca acacttgtaa
     9721 aagtgtatca aaacaaagga ctttaacttc ctttagaatg aacacttatg aaaaatatgg
     9781 gggtggggga gggtcaacta ttcgcgcact ctatcctgca atcagcaggt tttgcgcgaa
     9841 gggccactcg cggtcgatcc actggccttc gcaacggaat cctgtgcgtg cggccatctc
     9901 tataatttct tcggcctggt acttgtgcga gctttccgtc cagatggttt cgtcttcgtc
     9961 cagcatcagc cgcaggctgg cggccggaat gtgcaccgtt tgccggcgca gggagtgcaa
    10021 atgcatttca atccgccgtt ccaccctgtt ccatcgggct tcatggcgga agcagctcaa
    10081 atcaaaatcc gcgcctagct cccgattgat gcgtgccaac aaattcaagt tgaacgccgc
    10141 cgtcacgccc gcgggatcgt cgtaagccag caattgcagc tcgacgctct tctccaggtc
    10201 cgtgccgagc agcaacgcat cccccggctg gagaatctcc ctcatttcac ttaaaaatgt
    10261 ttcccccgca tagcgatcga aattaccgat cgtgctaccc aggaacagca cgagtagatg
    10321 gtcccgctcc cctcgcccat cggcaacggc acgcaatccc tccagatatg gctgctcgta
    10381 cccgacgacg ctgaccagat cgatctgccc gagttctttc gcgcacgctg ccagcgccga
    10441 aggcgaaatt tcaatggggt agtaatacgt catttgccgc cgcgcgagtg cttccagaat
    10501 ccaccgcgtc ttttttcccg agccgcttcc gagctcggcc acctgaatcg gcgacggcag
    10561 cctgcccaca atttcacccg cgtatttttc cagcagtcgc gcgtcggcgc gcgtcagtcc
    10621 gtactccggc aagacgcaaa tcgtttcaaa cagcgccgag cccacttcgt cgtacaagta
    10681 ctttgaagga agttctctct ggcctggctt cgtgaggctc tcgcgcactt cagacgcgaa
    10741 ttcagacaca tgccggagcg atagtgacag tgcaggcgtc gccatggggt ctcccgagct
    10801 tggggttgat tagtcctcga cgcaacggaa cgttgcgtag acgtacggat aatgcggctg
    10861 gaaccagttt cggaacgacc gccgcagcat gcacgcagca gtgcgcggcg agccaccctt
    10921 catcacgtag tgcttgccgt caaaaaaatt tgcagaatag ttggagtaga atggcatcgc
    10981 cgtaaaccct ggaaacggcg caaactccgt ccgcgtccac tcccacccgt tccccacaag
    11041 atcgtgcacg ccgaaagcgc tcgctcccgc gggatgcgct cccaccggcg a
//
LOCUS       DASYVO010000250         4054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_133843, whole genome shotgun sequence.
ACCESSION   DASYVO010000250 DASYVO010000000
VERSION     DASYVO010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133843"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VGR03_08375"
     CDS             complement(<1..258)
                     /locus_tag="VGR03_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEV1994331.1"
                     /translation="MERYPARDLLKVNEPVRRALAARYPRILVDEFQDTDPLQAEIIW
                     RLAGEGDVALPWHDASLPAARLYVAETCGPCAELRRWFKRQG"
     gene            443..2197
                     /gene="kaiC"
                     /locus_tag="VGR03_08380"
     CDS             443..2197
                     /gene="kaiC"
                     /locus_tag="VGR03_08380"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HEV1994332.1"
                     /translation="MAQKKKASQPSSKTLPKASTGIQGLDEITGGGLPRGRPTLVSGG
                     AGSGKTLFGLEFLVRGATQYSEPGVFMSFEESIPDLTKNVASLGFDLNRLVADKKLFV
                     DHVSITQSDLGETGEYDLEGLFIRIADAVQRVGARRVVLDTIETLFGNLPNPGILRAE
                     IHRLFNWLKQKGLTTVITAERDHPDKLTRHGIEEFVSDCVILLDHRIREEISTRRLRI
                     IKYRGSTHGTNEYPFLIDDQGISVLPISSLGLNHDASNERVSSGVARLDGMLGAKGFY
                     RGSSILASGTAGTGKTTIAGCFVDAACRRGERALFFAFEESPQQIVRNMRSIGIDLEP
                     WVRKGLLQFHAARPTYGGIEQVLLLTHKHITSFRPSVVVVDPVTNLLMVSTQNEVRSM
                     LTRLVDFLKTQKITAIFTSLTAAGALEASEADVSSLMDTWLLLKNIEVGGERNRALYV
                     LKSRGMEHSNQIREFVLTDDGLRLLDVYLGPEGVLTGSARVSQEMREKAVVTFRRQEQ
                     ETHRRELERKRRIFEARMVMLRAEFEAEEEVIQQTISESELLIEEVVQDRGQMVLSRK
                     ADSSSYKKDVRAKAARRR"
     gene            2198..2533
                     /locus_tag="VGR03_08385"
     CDS             2198..2533
                     /locus_tag="VGR03_08385"
                     /inference="COORDINATES: protein motif:HMM:NF019309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEV1994333.1"
                     /translation="MPTTKTKTRARPRKPSPRKPVVWNLRLYVAGQTPKSIRAFANLK
                     VLCEEHLKGRYQIEVIDLRKNPQLARGDQIVAIPTLVRRLPPPVRTIIGDLSNSVRLL
                     VGLDLQKVD"
     gene            2554..2922
                     /locus_tag="VGR03_08390"
     CDS             2554..2922
                     /locus_tag="VGR03_08390"
                     /inference="COORDINATES: protein motif:HMM:NF019309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEV1994334.1"
                     /translation="MKNRPKKSSSAGKRKLGLAGELEHPGSHSRQAEYGLRLYVSGST
                     LKSALAVENIKRICEQYLKNRYDLEVIDIYQQPNLARDEQIVAVPTLIKRLPPPMRRL
                     VGDLSNLKKVLFGLDLGMRE"
     gene            2947..>4054
                     /locus_tag="VGR03_08395"
     CDS             2947..>4054
                     /locus_tag="VGR03_08395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV1994335.1"
                     /translation="MADRKKNRSPQKLPALSRRELVARLSEAEEALRAIRSGEVDAIV
                     VNRQGEEKVFTLKGADHTYRVFVERMNEGAAVLSSDHIVLHCNGRFARFLGAGLQSVI
                     GSSMLDSVWPDDHPKLDALLRRAAQRNCRGEIRLQSRNGAPLSVHLSLNPLRLGSTRA
                     VCLIASDLSETKRAEQELRASSEQLRNLAAHLLSVREEERARISREVHDELGQSLTAV
                     KMDLAWLAGRLPQRNGQMLKRIRSTRQLADSIIQSIRRISTELRPAVLDLGLAAAVEW
                     QVQEFQARSGIQCTVRLLTREEVASNASTAMFRIFQETLTNVARHAKATRAEVVLQKQ
                     RDRLVLLIRDNGRGFDQADPSLSKSLGLLGMRERA"
BASE COUNT          866 a         1183 c         1240 g          765 t
ORIGIN      
        1 cccctgccgc ttgaaccagc ggcgcaactc cgcacacggt ccgcaggttt ccgcgacgta
       61 cagccgcgcc gccggcaaac tggcgtcgtg ccacggcagc gccacatccc cctcaccggc
      121 gagacgccag atgatctcgg cctgcaatgg gtcggtatct tggaactcat cgaccaagat
      181 tcgtgggtag cgcgcggcca gagctcgacg cacgggctcg ttcaccttga ggagatcgcg
      241 tgcgggatag cgctccacgc tccggtcttt atatttacgc gattgcgcgt atcctggtag
      301 attctgtagc tgcttttcta gagaagcagt ctatggtgaa gggaccgagt ggcccccttt
      361 tggtaagggc gacccgcgag gcggggcgat ggcgccgcca ctgccaaccc gccgtagatc
      421 gcccggggca aggatgaagc aaatggcaca aaagaagaaa gcaagccagc catcttccaa
      481 aacgctgccg aaagcatcca ccggcatcca gggtttggac gaaatcacgg ggggagggtt
      541 gccacgcggc cgtcccactc ttgtcagtgg aggcgccggt tccgggaaga cactgttcgg
      601 actggagttc ctggttcggg gcgctacaca atacagcgaa cccggcgtgt tcatgtcgtt
      661 cgaggaatcg atcccagacc tgaccaagaa tgtcgcttcg ctgggtttcg acctgaaccg
      721 gctggtggcg gacaagaaac tgttcgtgga ccacgtgtcc atcacgcaaa gcgacttggg
      781 ggaaaccggc gagtatgacc tcgagggcct cttcattcga attgccgacg cagtccagag
      841 ggtcggggcg cggcgagtcg tcctggacac cattgaaacg ctcttcggca atttgccaaa
      901 cccaggtatc ctgcgcgccg aaatccaccg cctgttcaac tggctcaagc aaaaaggatt
      961 gaccacggtc attaccgccg agcgggacca ccccgacaag ctgacccggc acggcataga
     1021 ggagttcgtc tcagactgcg tgatcctgct ggaccatcgt atccgtgagg agatctcgac
     1081 ccggcgtctg cgtatcatca agtaccgggg atcgacgcac ggcacgaacg agtatccgtt
     1141 cctcattgat gaccaaggca tttcagtcct gccgatctcc tcactgggcc tcaaccacga
     1201 tgcgtctaac gaacgagtct cgagcggcgt tgcccggttg gatggcatgc tgggcgccaa
     1261 gggcttttat cgggggagca gcattctggc atccggcacc gcgggaaccg gtaaaaccac
     1321 cattgccgga tgtttcgtgg atgcggcctg ccgccgcggt gagcgcgctt tgtttttcgc
     1381 tttcgaagaa tcaccccagc agatcgtccg caatatgcgt tcgatcggca tcgaccttga
     1441 gccatgggtc cgcaaagggc tcctgcaatt ccatgcggcg cgccccacgt acggggggat
     1501 cgaacaggtt ctgttactga cccacaaaca catcaccagc ttccggccca gcgtggttgt
     1561 ggtggacccg gttaccaatc tgctcatggt cagcacccag aacgaagtgc ggtccatgct
     1621 cacacggttg gtcgattttc tgaagaccca gaagatcacc gcgattttca ccagtttgac
     1681 tgcggcgggg gccttggaag ccagcgaggc ggacgtctcc tcccttatgg acacctggct
     1741 cctgctgaaa aacatcgagg tcgggggaga gcggaaccgc gcgctctacg tgctgaaatc
     1801 gagaggaatg gagcattcca accagattcg cgaattcgtg ctcaccgacg acggcctacg
     1861 attgctggac gtttacctcg gacccgaggg cgtgctgacc ggttccgccc gggtgtcgca
     1921 ggaaatgcgc gagaaagcag tggtcacgtt ccgccggcaa gaacaggaga cccaccggcg
     1981 cgaactggag cgcaagcgcc gcatctttga ggcccgcatg gtcatgctcc gggcggagtt
     2041 tgaagcggag gaagaagtaa ttcaacagac catctccgaa tcggaattac tcatcgagga
     2101 agtggtacag gatcgtgggc agatggtgct gagccggaaa gcggactcct cctcctacaa
     2161 gaaggatgtg cgcgcgaaag ccgcgcggag acgctaaatg ccgacaacca agacaaaaac
     2221 tcgagcgcgg cctcgcaaac cgtccccccg gaaaccggtg gtttggaatc tgcggctcta
     2281 cgtggcgggc cagactccca aatccattcg cgcctttgcc aacctcaagg tgctgtgtga
     2341 ggagcacttg aagggccgct atcagatcga agtgatcgac ctgaggaaga atccccaact
     2401 ggcgcgcggc gaccagatcg tagcaattcc gaccctggta agaaggcttc cgccaccggt
     2461 tcgaacgatc attggagacc tgtccaatag cgtgcggctg ttggtgggcc tggatctgca
     2521 aaaggtagac tgaccgagat caggaatgcc tacatgaaaa accgcccgaa aaagagcagc
     2581 tcggcaggaa agcggaaact cggattggcc ggggaattgg agcaccccgg ctcccattct
     2641 cgccaggcag agtacggctt gcgtttgtac gtaagcggat ccacgttgaa atcggcgctg
     2701 gctgtagaga atatcaagcg gatttgtgag cagtacttga agaatcggta tgacttggaa
     2761 gtcatcgaca tttaccagca gccgaatctg gccagggacg agcagattgt ggcggtgccc
     2821 acgctcatta aacggctccc gcccccgatg cgaaggctcg ttggggacct gtcgaaccta
     2881 aaaaaagtgc tcttcggact ggacctggga atgcgcgagt aatagctggc ggaaggcgga
     2941 aaaaggttgg ctgacaggaa gaaaaaccgc tccccgcaaa aactgccagc cctttcgcgc
     3001 cgggaattgg tcgcacgcct gagcgaagcc gaggaagccc tccgtgccat tcgttccggc
     3061 gaagtggatg ccattgttgt gaaccgccaa ggcgaggaga aagtgttcac gctgaagggg
     3121 gccgatcata cctaccgtgt tttcgtggag aggatgaacg aaggcgccgc tgttctcagc
     3181 agtgaccaca tcgtcttgca ctgcaacggc cgttttgccc ggttcctggg agcggggctc
     3241 cagagcgtca ttggttcttc catgctggac tcggtttggc ccgacgatca tcccaaactg
     3301 gatgccctac tccggcgcgc ggcccagagg aactgccggg gcgagatccg tttacaatcg
     3361 cgaaatggcg cccctttgtc cgtccatctc tccttgaatc ctctccgcct gggcagcacg
     3421 cgggctgtct gcctgatcgc ttcggacctg tccgagacga agcgcgccga gcaggaactg
     3481 cgtgcttcca gcgagcagtt gcggaatctc gcagcgcacc tgctgtccgt tcgagaggag
     3541 gagcgggcca ggatctcccg agaagtccac gatgaattgg gccagtcgtt gaccgcggtg
     3601 aaaatggatc tcgcctggct ggcggggcgg ctgccgcaga gaaacggcca gatgctcaaa
     3661 aggatccgct ccacgcggca gttggccgac agcattatcc aatccattcg cagaatctcg
     3721 acagagctaa ggcccgctgt tttggatctg ggtttggcgg ccgccgtgga atggcaggtc
     3781 caggaattcc aggcccgatc cggcattcag tgcacggtcc ggctgctcac ccgggaagag
     3841 gttgcgtcga acgcttcgac cgccatgttt cgtattttcc aggagacttt gaccaatgtc
     3901 gcgcgccatg ccaaggccac gcgggccgag gtggtcctac agaagcaacg ggaccggctg
     3961 gttctgctga tacgcgataa tggccggggt tttgatcagg cggacccttc cctttccaaa
     4021 tccctgggtc tgctgggtat gcgggaacgc gccg
//
LOCUS       DASYVO010000251         5502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_34643, whole genome shotgun sequence.
ACCESSION   DASYVO010000251 DASYVO010000000
VERSION     DASYVO010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34643"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="VGR03_08400"
     CDS             <1..1061
                     /locus_tag="VGR03_08400"
                     /inference="COORDINATES: protein motif:HMM:NF023591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994336.1"
                     /translation="ALEELVGAISEGSADRALGLVHTFQKEGRNLQHFCREAVRHMRN
                     LLIARTCGADSDLIAATPDQRPALAKAAALFSEEDLTRFFQILLQTDDDLRRKPDPRV
                     HLEMGLLRLINASRLAPLEELLAELKGGAPNSGANTGVARGAAPAGASATSPRSAAPS
                     FVAAIPAQPKAETKAQFSSPFAAEASAPPAAVAASTRVKRAVEAEESGIPAKSNGAVA
                     QAIGISQEHVAEIKTAIQAQQKFLGELVEQSSRWELEGAELRIYFLPENRTYAELLEG
                     RESLEKIRTISSKVLGRAVRVCAKLEAVAAAAASAARGASGTQELRAQFERDPLVRSM
                     LQRFGGKISEVKRSQEES"
     gene            1061..1387
                     /locus_tag="VGR03_08405"
     CDS             1061..1387
                     /locus_tag="VGR03_08405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV1994337.1"
                     /translation="MEVSALQQMLSRFRQLQEDLQRQVNSVTVEASAGGGMVTVKMNG
                     QKQIVDIRIEPDVFASKDQEMLQDLVRAAVNEASRRVDEELANQMKNLAGGIPGMAGM
                     KIPGLF"
     gene            complement(1497..2525)
                     /locus_tag="VGR03_08410"
     CDS             complement(1497..2525)
                     /locus_tag="VGR03_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994338.1"
                     /translation="MSAIKSSKILKWVGFATAILSLFFGVRELIKLVSDRIESHRKVD
                     AFLISEDVEMKGRDYASAWRTLEQASQLQPNSAKVHLAQENLAMAWLDDVHLRENEKF
                     SDIAERLEPALTRGIASTKDSQRSADLLAHIGWSHFLRSREGVFGLDPAGVYSKAAGQ
                     DKNNPYAEAMWGHWILWNHGKLSDALQHFSTALVSGRQRDFVRRLQLAALFNCPNDDC
                     AEEIIRVANDVRKEHGTVDPDAVNRIFSNYYNRIFSSSLAPNRILIAVPAAEHVATFR
                     WLFDGLDFDESKSMLRMGYLATLQEAAGQREEALASYRAVRAMTPKPRGLLSDAADAG
                     IKRLSPAR"
     gene            complement(2643..3554)
                     /locus_tag="VGR03_08415"
     CDS             complement(2643..3554)
                     /locus_tag="VGR03_08415"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEV1994339.1"
                     /translation="MKRSVVFVGFFLILGFALAGLYWSQRRPKSTPVSANAILNMAAD
                     AQRDLSRIPMHFTRLSDEQEIAIGKELASRYATQTRKLTPEEEGLEKYVTRVGGTVAV
                     HAHRHLPYTFTVLPDHNTINAFSLPGGPVYIGEGMLDLMMSEDELANVLAHEIEHIDH
                     YHCVERVQLEAKLKNLNLDILGALLQIPFDFWEAGYHKDEEFEADREGMYLAVQSGYS
                     PYGAVDMFERFAKLCDEYVIHAQSPDQELSELAIQSLNGYFRSHPLPSERLAQANRLI
                     AEQHWEDRKTQKPFRVEYEVHNGQFVK"
     gene            complement(3551..>5502)
                     /locus_tag="VGR03_08420"
     CDS             complement(3551..>5502)
                     /locus_tag="VGR03_08420"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEV1994340.1"
                     /translation="PLAFIAFAVVLSEAARALRYHALAWHAHLLTALALFTALTADQG
                     GTHAWHTIAVRAFSALPVVVGGYWLAKRLGTTDKRHLALARVAYTWAGTGMMVWVLQE
                     ALRAPWIAVGWIIFAVVLALSTRWICYQQLAWQANVVGLCALVRAYFYNYDLEQNFWG
                     PMSLRIFTISIVAAGLYFLSRKAAPEERYTRAIAFLHSFAATGLLALLAWYEAPNGWL
                     APLWASFALVLAIVDRRFELEELPWQSHILAALTLLRSISVNLYVTATWHGISVRLLS
                     LAIVAVIFYALSRLIRMPDEWRQRDLHHVYSWAASAIGGLLLWYELQTQPTSIAVAWG
                     AFGLVLFEYGLLHKITQFRYQAYLALVAAFTRIFFSNLTASEPGEFWGPRMYTILPLV
                     LIFFFVYAQLPHKEEDTSRDRRLHFDVLLAYLGTATIVALFYFQFPLEWVITSWAAVV
                     FALLGAALLLDRPIFLHQGLLLTVAVLGRGMAHNLFGDSYFGGGDWKGDYFVLSSAAA
                     ILLASLFFAFRLRGRDSAPQNANPWIRALAVVAGRPEQVVFFIPIILVTCMLALKMKS
                     GMVTVSWGVEGVLIFLLALAVKERSFRLTGLGILLLCVAKVIALDVWGLQPRDRYVTL
                     ITVGAALVFVSFLYTRYRDTIRQYL"
BASE COUNT         1269 a         1432 c         1785 g         1016 t
ORIGIN      
        1 acgcgctgga agaactggtc ggggcgatct cagaaggatc ggcggaccgc gcgctggggc
       61 tggtgcatac gttccagaaa gaaggccgca acctgcaaca cttctgccgg gaggcggtcc
      121 ggcacatgcg caatctgctg atcgcgcgaa cgtgcggtgc ggattcggat ttgattgcgg
      181 cgacgccgga tcagcgtccg gcactggcga aggcggcggc gctgttcagc gaagaggatc
      241 tgacgcgatt ttttcaaatt ctgctgcaaa cggatgatga tctacggcgg aagcccgatc
      301 cgcgggtgca tctggaaatg ggattgctgc ggctgattaa cgcgtcgcgg ctggcgccgc
      361 tagaggagtt gctcgccgaa ttgaagggcg gcgcgccgaa cagcggggcg aataccggag
      421 tggcacgcgg cgcggccccg gcgggagcgt ccgcaacatc tccgaggagc gcagcgccga
      481 gctttgtggc ggcgattccc gcccagccga aggcggaaac gaaagcgcaa ttctcttccc
      541 cgttcgctgc ggaagcatct gcacctccag cggcagttgc tgcttcgacg agagttaagc
      601 gggcggtgga ggcagaagaa tcgggaattc ctgcgaagag taatggcgcg gtggcgcagg
      661 caattggaat ctcgcaagaa catgttgcag agatcaagac ggcgattcag gcgcaacaga
      721 aattcctcgg cgaactggtg gaacagagca gccggtggga gctcgagggc gcggagttgc
      781 gaatttattt tttgccggag aatcggacgt acgcggaatt gctggaaggg cgcgagtctc
      841 tggagaagat ccgtaccatt tcgagcaagg tgctcggccg ggcggtgcgc gtctgtgcta
      901 aactggaagc agttgcagcg gcagccgcgt cggcggcgcg tggtgcgtcc ggcacgcaag
      961 agttgcgtgc gcagttcgag cgcgatccgc tggtgcggtc catgctgcag cgcttcggag
     1021 gaaaaatttc cgaagtgaaa cgaagccaag aggaatcctg atggaagtca gcgctctcca
     1081 acagatgcta tcgcgattcc ggcaactgca ggaagacctg cagcggcagg taaattccgt
     1141 gaccgtggaa gcatcggctg gcggcggcat ggtcacggtg aagatgaacg gccagaaaca
     1201 gattgtggac atacgcatcg agccggacgt tttcgctagc aaggaccagg agatgctgca
     1261 ggatttggtt cgcgcggcag tgaacgaagc ttcacggcgc gtcgatgagg aactcgccaa
     1321 tcagatgaaa aacctcgccg gcggcatccc cggaatggct ggcatgaaga tccccggact
     1381 tttttagcga acggatgctt ttcgctgcaa gacccactgc acacgatacc cagaaattga
     1441 tcctgtcgtg aagatgtaaa tccttctgct ccgttctctc gacaaaatct tccgaactag
     1501 cgagcgggcg agaggcgctt gatgccggcg tcggcagcat cggaaagcag gccacggggc
     1561 ttgggggtca ttgcgcgaac agcacgatag ctcgcgaggg cttcctctcg ctggccggcg
     1621 gcttcctgga gtgtagcgag gtagcccatg cgaagcatac ttttcgattc atcgaaatcg
     1681 agcccgtcaa acaaccagcg gaaagtggcg acatgttctg cagctgggac ggcgatcaga
     1741 atacgattgg gcgcgagact cgaagaaaag atccggttgt aataattgga aaagattcga
     1801 ttcacggcgt ccggatcaac tgtgccatgt tccttgcgga catcgtttgc gacacgaata
     1861 atttcctcag cacagtcatc gttcgggcaa ttgaagagcg cggccaattg cagtcggcga
     1921 acgaaatcgc gctgacggcc ggaaaccagc gcggttgaga agtgttgcaa ggcatcactc
     1981 aactttccgt gattccacaa gatccagtgc ccccacatgg cttccgcgta aggattgttc
     2041 ttatcctgcc ccgcagcttt ggaatagaca ccggcaggat ccaatccgaa cactccctcg
     2101 cgggaacgca gaaaatggga ccatccaata tgtgcgagca ggtcggcgga tctttgggaa
     2161 tcctttgtgg aggcgatgcc gcgggtcagt gcaggttcca gcctctctgc gatatcggaa
     2221 aatttttcat tttcgcgcag gtggacatca tcgagccagg ccatagccag gttttcctga
     2281 gcgagatgga ccttggcgga attgggttgc aattgggagg cttgttcgag ggtgcgccag
     2341 gccgacgcat agtcccggcc tttcatttcc acgtcttccg agatcaggaa ggcgtccact
     2401 tttcgatgag actcgatgcg atccgaaacc aatttgatga gctctcgaac gccgaagaag
     2461 agcgaaagga tagcggtcgc aaagcccacc cactttaaga ttttggagga cttgatggcg
     2521 ctcatggctt gccccgattc ttccacacta tgaagcagaa acaagcgtaa tcttcggcaa
     2581 actaccactt agtagcggaa atccttgacg agcatcacga tgacggaaac ggagcgatgc
     2641 cgctatttta cgaattggcc gttgtggact tcgtattcga cgcggaaggg cttctgcgtt
     2701 ttgcggtcct cccaatgttg ttcggcgatg aggcgattgg cttgcgcgag tcgctcggag
     2761 gggagcgggt gtgaacggaa ataaccgttc aaactctgaa tggccagttc ggagagctcc
     2821 tggtccgggc tttgcgcgtg aatgacgtat tcgtcgcaca atttggcgaa gcgctcgaac
     2881 atatcaacgg ctccgtacgg ggagtagccg gactgaaccg caagatacat gccttcgcga
     2941 tcggcttcga attcttcgtc cttgtggtag ccggcttccc aaaaatcgaa tggaatttgc
     3001 agtagcgctc caagaatatc gagattgaga ttcttgagtt tcgcttccaa ctgcacgcgc
     3061 tcgacgcagt gatagtggtc gatgtgctcg atctcgtggg cgaggacgtt ggcgagttcg
     3121 tcttcactca tcatcaggtc gagcatgcct tcgccgatgt agaccggtcc gccggggagg
     3181 gagaaggcgt tgatcgtgtt gtgatcgggg agaacggtga aggtgtaagg caaatgccga
     3241 tgcgcgtgaa ctgcaactgt acctccgacg cgggtgacgt acttttctag accttcttct
     3301 tcgggagtga gcttgcgtgt ttgcgttgcg taccgggagg caagctcctt gccgatggcg
     3361 atttcttgtt catcggagag gcgcgtgaag tgcatgggaa tgcgggagag atcgcgctgg
     3421 gcgtcggcgg ccatattcaa gatggcgttg gccgaaacgg gagtggactt ggggcggcgc
     3481 tggctccaat agagcccggc gagcgcgaag cccaggatga gaaaaaaacc gacgaagaca
     3541 acggagcgct tcataggtat tggcgaatcg tgtcgcggta tctggtgtag agaaacgaga
     3601 cgaataccag cgccgcaccc acggtgatta atgttacgta ccggtctcgc ggttgaaggc
     3661 cccagacatc gagagcaatg acttttgcga cgcaaagaag cagtatgccc aggccggtga
     3721 ggcggaagct gcgctctttg acggcgagcg ccagcagaaa gatcaacaca ccttcaactc
     3781 cccacgacac ggtgaccatg cccgacttca ttttcaaagc gagcatgcag gtgaccagga
     3841 taatgggaat gaaaaacact acttgttccg ggcgaccggc aaccactgca agagctctga
     3901 tccaggggtt cgcgttctga ggtgcgctgt cgcgcccgcg gagacggaaa gcgaagaaca
     3961 ggcttgccag caatatggcg gctgccgaac tgagcacaaa gtagtcgcct ttccaatcgc
     4021 ctccgccgaa atagctgtcg ccgaaaagat tgtgcgccat gccgcgaccg agaacggcga
     4081 cggtcagaag caatccctga tgcaggaaga tcgggcggtc gagcaggagc gcggcgccaa
     4141 gaagggcgaa cacgacggcg gcccaggagg tgatgaccca ttccagcgga aactgaaaat
     4201 agaaaagcgc cacgatggtg gctgtgccca agtacgccag gagaacgtcg aagtgcaagc
     4261 ggcgatcgcg gctggtgtct tcttccttgt gtggcaattg cgcgtacacg aaaaagaaaa
     4321 tcaggacgag cgggaggatg gtgtacattc gcggacccca gaattcgccg ggctcgcttg
     4381 cggtgagatt ggagaagaaa atccgggtga atgcggcgac gagcgcaagg tacgcctgat
     4441 aacggaactg cgtgatcttg tgcaacagtc cgtactcgaa gagcaccaga ccaaaagcgc
     4501 cccaggccac ggcgatgctg gtgggctgcg tttgcagttc ataccagagc agtaggccgc
     4561 cgatggcgga tgcggcccag gagtagacgt ggtggaggtc gcgctggcgc cactcgtcgg
     4621 gcatgcggat gaggcgcgag agcgcgtaga agattacggc gacaatggcc agcgacaaaa
     4681 ggcgcacgct gatgccgtgc caggtggccg tgacgtagag attcacgctg atgctgcgga
     4741 gcagcgtcag tgcagcgaga atatgcgatt gccagggcaa ttcttcgagc tcgaagcgcc
     4801 gatcgacgat cgcgaggacc aacgcaaacg aggcccagag cggtgccagc cagccgttcg
     4861 gtgcttcata ccaggccaga agcgctagga gtccggtggc cgcgaaagaa tgaagaaagg
     4921 cgatggcgcg ggtgtagcgc tcctccggcg cggctttacg cgagaggaaa tacaagcccg
     4981 cggcgacgat tgaaatggtg aagatgcgca gggacatggg gccccaaaaa ttttgttcaa
     5041 ggtcgtagtt gtagaagtag gcgcgaacca gagcgcaaag accaacgaca tttgcctgcc
     5101 aggcgagctg ctggtaacaa atccagcgcg tggaaagcgc gagcacaacc gcgaaaataa
     5161 tccagccgac ggcgatccag ggcgcgcgga gcgcttcctg aaggacccag accatcatgc
     5221 ccgtgccagc ccaggtgtag gccacgcgcg caagagcgag atggcgtttg tccgtggtgc
     5281 caagccgctt ggcgagccaa taaccgccga caacaaccgg caaggcgctg aatgcgcgca
     5341 cagctatggt gtgccaagcg tgggtgccgc cctgatcagc ggtgagcgcc gtgaataggg
     5401 cgagagcggt gagaagatgc gcatgccagg ctagcgcgtg ataacgcagc gcgcgcgcgg
     5461 cctcggacag aaccaccgcg aaagcgatga acgcgagggg ct
//
LOCUS       DASYVO010000252         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_188254, whole genome shotgun sequence.
ACCESSION   DASYVO010000252 DASYVO010000000
VERSION     DASYVO010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188254"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..695)
                     /locus_tag="VGR03_08425"
     CDS             complement(117..695)
                     /locus_tag="VGR03_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994341.1"
                     /translation="MSSPLASAIASLSSPEPSVRLAGAEEIYRLGRATAGSAVADWWA
                     ESELSGLLLGPNPAITVGLAVQRDTFGRIRIANGTPRLADVPPDQDAEEFELHFTEGI
                     SLDILTTREPGGSGAIAKYLAKFGEGVQQVEFLCANVDRVTQILKDKMGVDSVYPATR
                     HGADGTRVNFFLVASPDGGKVLIELYELQSRG"
     gene            801..2474
                     /locus_tag="VGR03_08430"
     CDS             801..2474
                     /locus_tag="VGR03_08430"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1994342.1"
                     /translation="MPKPVLLTVDDDPEVLRAIERDLRSRYADRYRVMRANSGSAGLA
                     TLRELKARNNPVALLLADQRMPQMDGVNFLSEAMEMHPLAKRALLTAYADTNAAIDAI
                     NEARVHYYLMKPWDPPEEKLFPALDDLLHDWTATFRPPYEGIRVLGTRWSNRSYELRE
                     FLARNQVPYQWIDVELAQSDAEVRGLINSLGPEAEKLPLILFPDGTRLPEPPLPAVAD
                     KIGLRTHALTTFYDLAIVGGGPAGLAAAVYGASEGLRTVIIEREAPGGQAGLSSRIEN
                     YLGFPSGLTGLDLARRAVAQARRFGVEILSPQEAVGLRAEGPYRYLKLADGSEISCHA
                     LLLAMGVQWRTLDVPGIGHLQGAGVYYGGGTSEALACKGETVYIIGGANSAGQAAMHF
                     SKFAERVVMLVRGVSLAATMSHYLIEQIEKTPNIEVWAHTSVVEVEGETRLTGITVEC
                     SPSGERKKLPATSLFIFIGAQPRTEWLGNAIERDERGFILSGPDLLHGGKRPASWTLA
                     RDPGLLETNVPGIFVVGDVRHGSVKRVASGVGEGAVVVQFMHQYLAKVQ"
     gene            complement(2507..>2777)
                     /locus_tag="VGR03_08435"
     CDS             complement(2507..>2777)
                     /locus_tag="VGR03_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994343.1"
                     /translation="SPRMSSSWKRQHTRDITRLGCKDWTALRLVFPPGSQLSFEHHEH
                     SVRWRVFLYGNFADVYMALFRLRDKPFQVGLRLVRERRHRPQFGD"
BASE COUNT          583 a          796 c          852 g          546 t
ORIGIN      
        1 ggctgcatcg cccaggcctg gaccgtcgcc gaagtcttgc gggcttggaa ggaaatatcg
       61 gacgggaaaa aacttggaac caattaaaag tacttgataa cgctgaattc gagcatctat
      121 ccgcgagact gcaattcgta cagttcgatg agcaccttac cgccatccgg agatgcgacg
      181 aggaaaaaat tgactcgcgt gccgtccgcg ccatgccggg tggcaggata aacagaatca
      241 actcccattt tgtctttcag tatttgcgtg acgcggtcaa catttgcgca gagaaactcc
      301 acctgctgca cgccctcccc gaacttcgcc agatattttg cgatggcccc ggagccgcct
      361 ggctcgcgcg tggtcagaat gtccagggaa atcccctcag tgaaatgaag ttcgaattcc
      421 tcagcatcct gatccggtgg cacatcggcg agcctgggcg tgccattggc aattcgaatt
      481 cgcccaaacg tatcgcgttg caccgcgagt ccaaccgtta tggcgggatt gggtccgagc
      541 agcagcccgg agagttcgct ctcggcccac cagtcagcaa cggcagatcc tgcggtcgcc
      601 cggcccaggc gatagatctc ctccgctccg gcgagcctaa ccgatggctc aggggacgac
      661 aggctcgcga tagctgaagc aaggggactg gacataaaga cgcaaggaat tgtagcttga
      721 gggcggcacg aaaagaagcc gaagccatgc ggcgatcctc ttaagacgca acgaaatcag
      781 gtgcgccgca tctgaggaac atgccaaagc cagttttatt aacagtggat gatgacccgg
      841 aagttctgcg ggccattgag cgcgatctgc gcagccgcta tgcggatcgc taccgcgtca
      901 tgcgcgcgaa ttccggaagc gccggccttg ccactttgcg cgaactgaaa gcgcgcaaca
      961 atcccgtggc tctgctgctc gcggatcagc gcatgcccca aatggacggt gttaacttcc
     1021 tatccgaggc gatggagatg cacccgctcg ccaaacgcgc actgctcacc gcgtatgcgg
     1081 acaccaacgc cgccatcgat gccatcaacg aagcccgcgt gcactactac ttgatgaagc
     1141 cgtgggatcc gcccgaggaa aaactctttc ccgccctcga cgatttgctt catgactgga
     1201 ccgcgacgtt tcgcccgcct tacgagggaa ttcgcgtcct cggcacgcgc tggtcgaatc
     1261 gttcctatga gttgcgcgaa tttctcgcgc gcaatcaggt gccctatcaa tggattgacg
     1321 tggagttggc gcagagcgat gccgaagtgc gcgggctgat taattcgctg ggacccgaag
     1381 cggaaaaact ccccttgatt ctgtttccgg atggcacgcg cctcccggag ccgcctctgc
     1441 ccgccgtcgc ggataagatt ggcctgcgca cgcatgccct aaccaccttc tacgatcttg
     1501 ctatcgtcgg cggaggcccg gcaggtcttg ccgccgctgt gtacggcgca agcgagggtt
     1561 tgcgcacggt gatcatcgag cgcgaggcgc ccggcggcca agccggactg agttctcgta
     1621 ttgagaacta tctcggtttt ccttccggac ttaccggcct cgacctggcg cggcgcgccg
     1681 ttgcgcaggc gcggcgcttc ggcgtggaaa ttctctcgcc gcaggaagcg gtcggtctcc
     1741 gcgcggaggg cccctacagg tatttgaagc ttgcggacgg ttcggagatt tcctgccacg
     1801 cgttgctact ggccatgggc gttcagtgga gaaccttgga cgtgccgggc attggtcacc
     1861 tgcaaggcgc gggtgtgtat tacggcggtg gaacttccga agcgctcgct tgcaagggcg
     1921 aaacggtcta catcattggc ggcgcaaatt ccgccgggca ggctgccatg cacttttcga
     1981 agtttgcaga gagagtagtg atgcttgtgc gcggagtgtc gcttgccgcc acaatgtcac
     2041 actacttaat cgagcaaatc gaaaaaacgc cgaacatcga agtgtgggcg cacacgagcg
     2101 tggtcgaagt ggaaggagaa acgcggctca cgggaattac cgtcgagtgc tctccctcgg
     2161 gagaaagaaa aaagctcccc gccacgagct tgttcatttt tatcggcgcg cagccgcgga
     2221 cggaatggct tggtaatgcg attgagcgcg acgagcgtgg gttcatcctc tccggcccgg
     2281 atctgttaca cggagggaag aggccggcgt cttggacgct ggctcgcgat cccggattgc
     2341 tcgaaaccaa tgtgcctgga attttcgttg tgggcgatgt gcgccatggt tccgtgaagc
     2401 gcgtcgcttc cggtgtcggc gaaggagcgg tggtcgtgca gttcatgcat cagtatctgg
     2461 cgaaggtcca atgagcgaaa cacaggatca agcacgtctg gaagcactag tcgccaaatt
     2521 gcggacggtg ccgccgttcg cggaccagcc gcaagccgac ctggaatggt ttgtcgcgca
     2581 aacggaagag cgccatgtaa acgtcggcga aattaccgta aaggaagaca cgccagcgga
     2641 cactatgttc gtgatgctcg aaggagagtt gcgagcccgg cgggaaaacg agcctcaagg
     2701 cggtccagtc tttgcagccc aggcgggtga tgtcacgggt gtgctgcctt ttccaggatg
     2761 aagacattcg gggtgac
//
LOCUS       DASYVO010000253        12271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_215711, whole genome shotgun sequence.
ACCESSION   DASYVO010000253 DASYVO010000000
VERSION     DASYVO010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 12271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12271
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215711"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VGR03_08440"
     CDS             complement(<1..313)
                     /locus_tag="VGR03_08440"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEV1994344.1"
                     /translation="MDLFEEIVKLRREGKRGALATIVHTNGSIPSFESSRMLVREDGT
                     IAGTIGGGCVEADVWAAAREVMQKEASRKMVFNLNHEANYDNGLICGGTLEVFVEPIL
                     PQ"
     gene            complement(481..2130)
                     /locus_tag="VGR03_08445"
     CDS             complement(481..2130)
                     /locus_tag="VGR03_08445"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HEV1994345.1"
                     /translation="METHPKGSRARILLSSVFGPYAQDDEFGSRAINPMELYHNQVTR
                     AQGSFSLRMFHRSWGIMLIQANISAPCTVLDFPTREAFARELTTNRYDVVGISSIIVN
                     VHKVREMCRMARELSPGSVIVVGGHVAAIPGLEATIDADHIVQGEGISWMRRYLGEDE
                     HAPIRHPAIVSSLQTRIMGVRIPQRKGGTAATIIPSVGCPMGCNFCTTSAFFGGKGKF
                     VNFYETGDELFDVMCRIESELKVHSFFVMDENFLLHRKRAMRLLERMKQAGKSWELSV
                     FASANAIRKYTMLELAELGVSWVWMGLESPASGYSKLRGTDIRQLTRELREHGIRVQG
                     STIIGLEHHTPDNIVQEIESAVGCQADFHQFMLYTPVPGTPLYQEMADQGRLLPDVDL
                     ADIHGQYKFNFRHAAISREDSKRFLDWAFWRDFERNGPSLYRMCRTMLQGWRRYKDSP
                     DPRVRERFARQKKKLSGAYNAALWVMEREFKKVNQSVSEQIHKLRREVEKEFPVVARL
                     TAASLGPILLWAARREENRLASGRTYEPPTFVERSNWISAS"
     gene            2619..3425
                     /locus_tag="VGR03_08450"
     CDS             2619..3425
                     /locus_tag="VGR03_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV1994346.1"
                     /translation="MKFQQMNARHYGAVQDSQDTSRKSFRVVRAGERTNVDGKPVTNK
                     VLLAIPDNEYQLIRPDLKYIDLPDHLSLHEPTQNIEFVYFPNRGMVSQVVVTKDGRTV
                     EVGVVGNEGYVGAGLAVGLSRSSVREIVQIGGDGSRIMGNALERILRSAPQLQTILSR
                     HTGLQGMQVAQTAACNRLHDIQQRLARWMLMTQDRVNLGVLPITHDFIATMMGTDRST
                     VSLAAAVLQKKGIIEYVRGAVKIVNRRKLQNSACECYEVIQQFEADLGLR"
     gene            4170..5198
                     /locus_tag="VGR03_08455"
     CDS             4170..5198
                     /locus_tag="VGR03_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV1994347.1"
                     /translation="MKAILFHKHGGPEVLQYTDAPEPVPGPRDVLVRVRACALNRLDL
                     WVRQGLPGVPIPLPHIPGSDVAGEITQVGRDVTRIKPGEKVVLAPGVTCGRCVACLSG
                     RDNLCREFTNLGYMIDGGCAEYVRCPEVNCMPYPQNLSFEHAAAIPLVFQTAWHMLVG
                     RARLQPGEDVLVLGAGSGVGSAAIQIAKFLGARVIATAGSDEKLAKARELGADETINH
                     RTQKIRDEVRRFTGKRGVDIVFEHVGTATWDESLASLAAGGRLVTCGATTGYDAKLDL
                     RFLFSRQLSLLGSYMGEKGELYTLLKLVAAGRLKPVVDRTFPLSACAEAHAYLEAGKQ
                     FGKVVLSV"
     gene            5340..5570
                     /locus_tag="VGR03_08460"
     CDS             5340..5570
                     /locus_tag="VGR03_08460"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEV1994348.1"
                     /translation="MYKVHQAKTNLSRLIKEACAGRKVIIARGDQPVVRLVPIPATGK
                     KRIPGRLKGQIRIAAGFFKPMSRKERKAWGLE"
     gene            5567..5950
                     /locus_tag="VGR03_08465"
     CDS             5567..5950
                     /locus_tag="VGR03_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEV1994349.1"
                     /translation="MNLLLDTHALLWWFDGSSRLSRRALRAIESQSNSVVVSAVSAWE
                     IAIKSAGGKLDAGPLLAGFRTEIEQEGFSELPISFEHAVRAGLLPGPHHDPFDRMLIA
                     QAQLENLAIVSRDDSFDRYSVRRIW"
     gene            6063..6602
                     /locus_tag="VGR03_08470"
     CDS             6063..6602
                     /locus_tag="VGR03_08470"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEV1994350.1"
                     /translation="MSISLGKHVTLTQPGDFGKLLEDEGILFTQLDKIVNWARRSSVW
                     PFSFGLACCAIEMMSMAAARFDVARFGAEVFRPSPRQADLMIVAGRVSQKMAPIIRQL
                     YAQMPEPKWVISMGACATSGGVFNNYAIVQGCNQVIPVDVYVPGCPPRPEALLYAILQ
                     LQKKIDSERGSFKRALNFA"
     gene            6680..7168
                     /locus_tag="VGR03_08475"
     CDS             6680..7168
                     /locus_tag="VGR03_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994351.1"
                     /translation="MATTPDPARALLRHTLATVAYRGGKALRGAPESFAAFAAGEKVR
                     TPAQILVHLGDLFDWALSIVRDQQAWRDSPPLPWNDEVARFFAALKAFDDVLASDAPL
                     RESPGKLFQGPIADALNHIGQIAILRRMAGCPIRGENYFKAEIVPGRVGADQAVPRRE
                     FD"
     gene            complement(7178..8047)
                     /locus_tag="VGR03_08480"
     CDS             complement(7178..8047)
                     /locus_tag="VGR03_08480"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEV1994352.1"
                     /translation="MASRTACREAITVSSRADPMFERFEERARRVIFFARYEASQYGS
                     PYIESEHLLLGLAREDPFLRSRIGQEQLRSEIEKHVTRAERISVAMEVPLSAECKQIL
                     VLAGEEADRLGSRAVGTEHLLLALLRVEESLAGKILREKKFSRELVLEKLPESRPGAQ
                     AAMHSSRAAVDALQNFLTGLRDKHPEQPASYFAESGQFIDTNGRRWVGREEIEKGFAG
                     LFGPYAKRRTSFQIEDTTQPSGEQFIATLLWRDAVSSGDPKPTMLRMSVVMALGAEYW
                     TIVLAQATFVNLR"
     gene            complement(8181..9281)
                     /locus_tag="VGR03_08485"
     CDS             complement(8181..9281)
                     /locus_tag="VGR03_08485"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEV1994353.1"
                     /translation="MTTETAKPTMETPLAKLHRAAGARMGVWFGCALPDAFGEWETEY
                     RFAKETVALIDKNYRAYFSFTGPDRVRYLNAILTNNIGGLKPGEGAVSLLLSPQGRIL
                     AEVETYALEDRILGVSSAMIRERLVETIERFIIMDDVTLADETERYGTLALEGPRSAE
                     IVREICGVELESLPELGHREARRGGSSLSCRVSRRSPGGVAGAEFLIERSHLVEWWEV
                     LQGAARAHGGGPIGYTALSALRLEQGVPWFGYDFDERQIPHEAGLQDSHISYTKGCYT
                     GQEIVERVRSRGQVNRVRVLLKFAGAEPPAAGTPLLVDGKEVGHVTRSAYSPALRCAI
                     AMGYVRREHSAPGSSIEAGGIAGSVLPGAAIV"
     gene            complement(9481..10962)
                     /locus_tag="VGR03_08490"
     CDS             complement(9481..10962)
                     /locus_tag="VGR03_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994354.1"
                     /translation="MVRMSKIRPLFFTLVLLLLPGFASAADNWIEVRSPHFTVATNSG
                     EKDARKIADQFEQIRGLFQRVFTTLRVDAGQPILILAAKNENTIKEFLPEYWEVKGRV
                     HLAGLYQPGQDKDYVVMRLNSEGDNPYHTLYHEYTHALLRLNYHDLPIWLNEGLAEFF
                     GNTTLGDKEITMGTFGPGQLYVLNQNKLLPIETLFQVDQGSPYYNEQDRASVFYAQSW
                     AVVHYFLMDPEARQKQYLKRFLSAFDKSGNQIDAAREALGDLKEFGRRIDAYARRSSL
                     NVSVLKNSAESTGKNYAVRNLSPGEVLALRGDFFAHHNRLDRAKPVLEEAVKLEPNLA
                     FAHESLGYYHYRLREVKEADKEVQEALRLGATDFIAPYYHGLFLFQSDFGNADAVKEA
                     TDSLLKATQLNPRFAPAYDALANAYSRFPETRNQALDAAAKAAQLDPATLWYTAHLIQ
                     LLLNGNQVEQARPIAERLARAARSPADQKMAQDLLRRVKERQD"
     gene            11088..11786
                     /locus_tag="VGR03_08495"
     CDS             11088..11786
                     /locus_tag="VGR03_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1994355.1"
                     /translation="MKESSAAPHASGGALIHEIIPVGWLQCNCSIIGDPATREALVLD
                     PGDEVERIEEILKRHQLTVKAIVSTHAHIDHVGGLKKLHDLTGAPVMMHADDLQLYQA
                     LDRQAKWLGMRAPAMTEVHQLLKEGDALRWGPFEAHVMHTPGHTPGSVCLYLPRGAGK
                     IALKLPHLFAGDTLFAGSIGRTDLWGGSFEEIMRSLRGKLLALPDETLVFPGHGPKTT
                     IGAERDSNPFLLVK"
     gene            complement(11859..>12271)
                     /locus_tag="VGR03_08500"
     CDS             complement(11859..>12271)
                     /locus_tag="VGR03_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994356.1"
                     /translation="SEVVSRMGATLRIMFRAPDGVKLRAKIVCSEWYGPAGLNRSVKE
                     GAMTAFEEHQEDLEKYERMFGPARGRLAVSLDRVTNALVLVGQHGVYCRSQRNAAVPA
                     MDIQIITQELGHAKELIQSVMEELRRSQAPGERQ"
BASE COUNT         2384 a         3887 c         3513 g         2487 t
ORIGIN      
        1 gttgcggcag aatcggctcc acgaaaacct ccagcgtgcc gccgcagatc agcccgttat
       61 cgtagttcgc ctcgtggttc aggttgaaga ccattttgcg cgaggcttct ttctgcatca
      121 cctcgcgcgc cgccgcccac acgtccgcct cgacgcagcc gccgccgatg gttccggcga
      181 tcgtgccatc ctcccgcacc agcatgcgcg agctctcaaa gctcgggatg gagccgttcg
      241 tgtgcacaat cgtggccagc gcaccgcgtt tcccctcgcg ccgcagcttc acgatttcct
      301 caaagagatc catgcatgtt ccctgcattt acgctacgcc gcgttccgtg gcctgtcaac
      361 gctcgcggtg ttccggtggt ggttcagtct gcgagtcgcc agcaactctc cagtattgta
      421 cgagtggcag ggacctcgcc ggggatgggc gctgttaaag acggccacga ggcaggaggg
      481 ctacgaagcc gaaatccaat tgctgcgctc gacaaaggtg gggggttcgt aggtacgccc
      541 tgacgccagg cgattttcct cccggcgtgc ggcccacaac aagatagggc ccagcgacgc
      601 ggccgtgagg cgggcgacga ccgggaattc cttctcgact tcgcggcgca gcttgtgaat
      661 ctgctcactc acgctttgat tgactttctt gaattcccgc tccatcaccc aaagcgccgc
      721 attgtaagcg ccgctgagtt tcttcttttg ccgcgcaaac cgctcgcgga cgcgtggatc
      781 gggggagtcc ttgtagcgcc gccagccctg gagcatggtc cggcacatgc gataaagact
      841 cggcccattc cgctcgaagt cgcgccagaa cgcccaatcc aggaacctct ttgaatcttc
      901 gcgggagatt gcggcgtgcc gaaagttgaa tttgtactgt ccatggatat cggcgaggtc
      961 cacgtccggg aggaggcgac cctgatcggc catttcctgg taaagcggcg tgccgggaac
     1021 cggcgtgtag agcatgaact ggtggaagtc ggcctggcag ccgaccgccg attcgatttc
     1081 ctggacgatg ttgtccggcg tgtggtgctc cagtccgatg atcgtcgaac cctgcacccg
     1141 aatgccgtgt tcgcgaagct cccgagtgag ctgccgaatg tccgtacccc gcagcttgct
     1201 ataaccggac gcgggagatt ccaagcccat ccatacccaa gaaacgccga gttcggccag
     1261 ttccagcatg gtgtattttc gaatcgcatt ggcagaagca aagaccgaaa gctcccagct
     1321 cttgcccgcc tgcttcatgc gctccagcaa gcgcatggca cgtttgcggt gaagcaggaa
     1381 gttctcgtcc atcacaaaga aggaatgtac tttgagctcg gattcgatcc ggcacatcac
     1441 gtcaaacagt tcatcgccgg tctcgtaaaa gttgacaaac tttcccttgc cgccaaaaaa
     1501 agcagaagtg gtgcagaagt tgcagcccat ggggcagccc accgacggga tgatggttgc
     1561 tgccgtcccg cccttccgtt gagggatgcg cactcccatg atgcgcgtct gaagactgga
     1621 aacaatggcg ggatggcgga tcggcgcgtg ctcgtcttct cccaagtacc ggcgcatcca
     1681 ggagatacct tctccctgca cgatgtggtc ggcgtcgatt gtcgcttcca ggccgggaat
     1741 ggcggctacg tgtccgccca ccacgatcac cgaacccggg gacagttccc gcgccattcg
     1801 gcacatctcc cggaccttgt gcacgttcac gatgatcgaa gagatcccaa cgacgtcgta
     1861 gcgattcgtg gttagttcac gagcaaaggc ttcccgcgtg ggaaaatcca gcacggtgca
     1921 gggtgccgaa atattcgcct gaatcagcat gatgccccag gagcgatgaa acatgcgcag
     1981 cgagaagctg ccttgcgcgc gggtgacctg gttgtgataa agctccatgg gattgatggc
     2041 gcggctgcca aattcgtcgt cttgcgcata aggcccgaag accgaactca acaggatacg
     2101 agcgcgactt ccctttgggt gtgtttccat aggatccctg tgtggagtgt acccctcggc
     2161 agagttcttc gcggtgatcg cggtcacatc acaatgctat tgcgtgtcac caaaacagat
     2221 ccctgggacg aagttcgtac gggatggaga acgcgtttgg gactctgcct tgcctaagtt
     2281 cgcaggatcc gcccgcaatc ggcagcaagc tccaaatgga gcttccaact tcccgcaagc
     2341 ggagttatcg ggagtcagcg aaagttcaat tgtcgcgtaa gagcgttaat cgggacccat
     2401 ccaaatatgt caactgcaga aggcttattg gctacgatgc gcgcggagaa ggtttggtcc
     2461 aagcacgtcg agaatccgcg gcaagggtac cggatcccct tgccaacgcg ccttgatctg
     2521 ccgcatgatt cgctccgcct tggttttgag aggtgtgccg tttcgcatag cttgttagac
     2581 ccctcttttg gttaaatgga cgtggagtct cagagcccat gaagtttcaa caaatgaatg
     2641 caaggcacta tggtgccgtt caggattccc aagacacgag tcgaaaaagc tttcgagtcg
     2701 tacgagcggg cgaacggacc aacgtcgatg ggaagccggt aaccaacaaa gtgctgttgg
     2761 cgatacccga taatgaatac cagttgattc gtcccgactt gaaatacatc gatctgccgg
     2821 atcatcttag cctgcatgag ccgacgcaaa acatcgagtt cgtctatttt ccgaaccgtg
     2881 gaatggtttc tcaggttgtc gtgaccaaag acggtcgaac cgtggaagtg ggtgttgtgg
     2941 ggaacgaagg ctatgttggg gcaggattgg cagttggctt gagcagaagt tcagtgcgcg
     3001 aaatcgtcca aatcggcggc gatggctccc gaataatggg aaatgcgctg gaacgcattc
     3061 tgcggtcggc tccgcagttg cagacgatct tgagcaggca tacggggctt caagggatgc
     3121 aagtcgcaca gaccgcggcg tgcaatcggc tccacgatat acagcagcgt ctcgcgcggt
     3181 ggatgctgat gactcaggat agggtgaatt tgggagtact tccgatcacc cacgatttca
     3241 ttgcgaccat gatgggtacg gaccgatcca ctgtgagcct ggcagcggcc gtgctgcaaa
     3301 aaaagggaat catagaatac gtgcgaggtg cagtgaagat tgtgaaccgc aggaaactcc
     3361 agaactccgc ctgtgagtgt tacgaagtaa ttcaacagtt cgaggctgac ctcggattga
     3421 gatagcattt tttgtcagcg ttgggtaccc cacagacagc aatctctttc catcgttaat
     3481 gttcaagctt ggtgtatcca cttcggttgc gatgtgcgtt caagaggggt gattttggca
     3541 gaacctcgct cagggcaatg aaaggagcgg cctttgtcgt cgcagtgccg tcgaagacca
     3601 atgctagacc gtctttcaca gaagcataac cacactaact cgaggtgtgc catggaggcg
     3661 acgcgtcgca agctggtatg ggtcgaaaga gagaattttc agggctgggc ttgcaccgta
     3721 tgcgcatggg cgttcaaccc tttagggccg gtcgttggcg aatccattga cgagatgaag
     3781 atgcgctacg agcggcagcg cgacacggag ttcacatctc atgtctgtgc cgagcacccg
     3841 agagcagcaa caaatccacg ttagtcaaat cactcgcggg ctaagtactt cgtgattctc
     3901 gtgttcatgc aaagcaacca cccttcaaac tgaatttcga atgggttgaa accgttcgag
     3961 acttgcaagc cgccgaggcc aggatccaag aactacaagc gcgttcgccc ggcgaatgcg
     4021 tggtcttcag ccagcgcacc caacagattg ttgcgaggtt cgactctctg atggcaggcg
     4081 cgtcctcggc ctgtcaacgc tcgccgcttt ctggacaccc ctgtgtccct cacttggcgt
     4141 agaatccctc ttcgcattca ggagcgctca tgaaagccat cctgttccac aagcacggcg
     4201 ggcccgaagt cctgcagtac acagatgcgc ctgagccagt acccggcccg cgcgacgtgc
     4261 tcgtgcgcgt ccgagcctgc gcgctgaatc gtctcgacct gtgggtccgc caaggcctgc
     4321 ccggcgttcc gattcctctc ccgcacatcc ccggcagcga cgtcgccggc gaaatcaccc
     4381 aggtcggcag ggacgtgacg cgcatcaagc ccggcgaaaa agttgtcctc gcaccagggg
     4441 tcacctgcgg ccgatgcgtg gcctgcctct ccggccgcga taatctctgc cgcgaattca
     4501 ccaatctcgg ctacatgatt gacggcggct gcgccgagta cgtccgctgt ccggaagtaa
     4561 actgcatgcc ttacccgcag aatctttctt tcgagcatgc cgccgccatc ccgctcgtct
     4621 tccagacggc gtggcacatg ctcgtcgggc gcgcgcggct gcaacccggc gaagatgtcc
     4681 tggtgctcgg cgcgggcagc ggagtcggca gcgcggccat ccagatcgcc aagtttctcg
     4741 gagcgcgcgt catcgccacc gccggcagcg acgaaaagct cgccaaggcg cgcgagctcg
     4801 gcgccgatga aacgatcaac caccgcacgc agaaaattcg cgacgaagtc cgccgcttca
     4861 ccggcaagcg cggcgtggac atcgtcttcg agcatgtcgg caccgccacg tgggacgaga
     4921 gcctcgccag cctcgcggcc ggcgggcgcc tggtcacttg cggcgccacc accggctacg
     4981 acgcgaagct cgatctccgc ttcctcttca gccggcagct ctcgcttctc ggctcctaca
     5041 tgggcgagaa aggcgagctg tacacgctgc tgaagctcgt cgccgcgggc cgcctcaagc
     5101 ccgtcgtgga ccggacgttc ccgctctccg cctgcgccga ggctcacgcg tatctcgaag
     5161 cgggaaaaca attcggcaaa gtcgtcctct ctgtgtaatg ggaggcagcc cgcccccttt
     5221 ttgcgctgcg acccagagcg ctgccgagtt cctttcggcg caggccacgc tccgccacaa
     5281 aggacgggtc tcaaagtctc cttgactcgg ccgaccctag ctaatatact tagctaatca
     5341 tgtacaaggt ccaccaggcc aaaacaaacc tctcccggct gatcaaggaa gcttgcgccg
     5401 gccgcaaagt catcatcgcc cgcggtgacc agcccgtcgt ccgcctcgtg cccattcccg
     5461 ccaccggcaa gaaacgcatt cccggccgcc tcaaagggca gattcgcatc gccgcaggct
     5521 tcttcaaacc catgtcgcgc aaggagcgca aggcatgggg tttggaatga acttactcct
     5581 cgacacacac gcccttctct ggtggtttga cggaagcagc cgtctctccc ggcgcgccct
     5641 gcgtgccata gagagccagt ccaactccgt tgtcgtttcc gcagtttcgg cctgggaaat
     5701 cgccatcaag tccgctggag gcaagctcga cgccggtcct ctgctcgctg gtttccgcac
     5761 cgagatcgaa caggaagggt tttccgaact tccaatttct tttgagcacg ccgtacgcgc
     5821 cgggctactg cccggcccgc accacgatcc attcgaccgc atgctcatcg cgcaggccca
     5881 gctcgagaat ctcgccattg tcagccgcga cgattccttc gaccgctact ccgtccgccg
     5941 catctggtaa cccccctcgg cccggccagc attccctgtg gcgtcctctc cgtgcagcgc
     6001 tccgcactcc ccacgattgt gcctccaggc gggctgtgcg aaaatagcaa ttggacgctc
     6061 ccatgagcat ctccctcggc aaacacgtca cgctgaccca gcccggcgac ttcggcaagc
     6121 tcctcgagga cgagggcatc ctcttcacgc agctcgacaa gatcgtcaac tgggcgcgca
     6181 ggagctcggt atggcccttc agcttcggtc tcgcctgctg cgccatcgag atgatgtcca
     6241 tggccgccgc acgcttcgac gttgcgcgct tcggcgccga agtcttccgc ccctcgccgc
     6301 gccaagccga cttgatgatc gtggccggcc gcgtgtcgca gaaaatggcg ccgatcatcc
     6361 gccagctcta cgcgcagatg cccgagccga agtgggtcat ctccatgggc gcctgcgcca
     6421 cttccggcgg cgtcttcaac aattacgcca tcgtgcaggg ctgcaatcag gtgattcccg
     6481 ttgacgttta cgtgccgggc tgcccgcccc gcccggaggc gctgctctac gccatcctgc
     6541 agctacagaa gaagattgac agcgagcgcg gctcgttcaa gcgcgccctc aacttcgcct
     6601 gatctcgaga tctgttcgtg caggggcgaa ctgaagtccg ccctcgtatt cttcattctc
     6661 ggaaaggacg cgtcgcccca tggccacaac acccgatccc gctcgcgctc tcctccgcca
     6721 cacgctcgcc acggtcgctt atcgcggggg caaagccctg cgcggcgcgc cggagtcgtt
     6781 cgctgccttc gcggccggag aaaaggtccg cacgcccgcc cagatactcg ttcacctcgg
     6841 cgatctcttt gactgggctt tgtccatcgt ccgcgaccag caggcctggc gggattcgcc
     6901 gccgcttccc tggaacgacg aggtggcgcg cttcttcgcc gcgctcaaag ccttcgacga
     6961 cgttctcgcc tccgatgcgc ctctgcggga gtcacccgga aagctcttcc agggccccat
     7021 cgccgacgcg ctgaaccata tcggccagat cgccatcctg cgccgcatgg ccggctgtcc
     7081 cattcgcggg gagaattatt tcaaagcgga aatcgtcccc ggccgcgtcg gcgcggatca
     7141 agccgtccct cgccgcgaat ttgattgaga tggcatctta gcggaggttg acgaatgtag
     7201 cctgggctaa aactatcgtc cagtattcgg cccctagcgc catcaccacg ctcatgcgaa
     7261 gcattgtcgg cttcgggtcc cccgatgaaa cagcatctct ccacagaagt gtggcaatga
     7321 actgttctcc tgagggttgg gtcgtatctt cgatctgaaa gctggtcctc ctctttgcat
     7381 aggggccaaa taggccagcg aatccctttt cgatctcttc ccgcccgacc caccgtcgcc
     7441 cgttcgtgtc aatgaactgc cccgattccg cgaagtagga agcaggctgt tcagggtgct
     7501 tgtctcgcag ccctgtgaga aaattctgca gagcgtccac ggcggcgcgg gaggaatgca
     7561 tcgccgcctg agcacctggt cgggattcgg gcaacttttc cagaactagt tcacgggaga
     7621 acttcttctc tcgcagaatc ttgcccgcga gggattcttc gacgcgcagc agggctagca
     7681 gaagatgttc agtgcccacc gccctgcttc ccaggcggtc agcttcctct ccggccagaa
     7741 ccagaatctg tttgcactcg gcgctcagcg gaacttccat tgcaaccgag attctctcgg
     7801 cgcgagtgac gtgcttctcg atttcgctgc gtagctgttc ttgaccaatt ctggagcgga
     7861 ggaaggggtc ttcgcgcgcc aaacccagaa ggagatgctc tgattcaatg tacgggctgc
     7921 catactggct tgcctcatac cttgcgaaga agattacccg gcgcgctctc tcctcaaatc
     7981 gctcaaacat agggtcggct cgcgaggaca ccgtgatggc ttcgcggcag gcggtacgac
     8041 tggccatgca aaatcagaat ccggtgtcca aatggcatcg aactgggaag tttagcgcgt
     8101 gatttgcgaa taatgaagaa ccatgtggcc gtgagcgctc tgaccgctgc ggcggagggc
     8161 acaacctggc tgccgtcgag tcagacgatc gccgcgcccg gcagcacact tcctgcaatc
     8221 ccgcccgctt caatgctgct tcccggcgcg ctgtgctccc ggcggacgta gcccatggcg
     8281 atcgcgcaac gcagggcagg ggagtacgcg ctccgcgtca cgtgtccgac ttccttgcca
     8341 tccaccagca acggcgtgcc cgctgcgggt ggctccgcgc cggcaaattt cagcagcacg
     8401 cgcacgcggt tcacctgccc gcgcgagcgc acgcgctcga caatctcctg gccggtgtag
     8461 cagccctttg tgtagctgat gtggctgtcc tgcaatcccg cttcgtgcgg aatctgacgt
     8521 tcatcgaagt cgtagccgaa ccacggcacg ccctgctcca gccgcagcgc gctcagcgcg
     8581 gtgtagccta ttgggccgcc gccgtgcgcc cgcgccgccc cttgcaaaac ttcccaccac
     8641 tcaacaaggt ggctgcgctc aatcaaaaat tccgcgcccg ctactccccc cggcgaccgc
     8701 cgcgacaccc ggcaggatag cgaagagccg ccgcgccgcg cctctcggtg gcccagctcc
     8761 ggcaggctct caagctcgac gccgcaaatc tcacggacga tctccgcgct gcgcggccct
     8821 tccagcgcca gcgtgccgta tctttcggtt tcgtccgcga gcgtgacgtc gtccatgatg
     8881 atgaaccgct caatcgtctc caccagccgc tcgcgaatca tcgcggagga aactcccagg
     8941 atgcggtctt ccagcgcgta cgtctcgacc tccgcgagaa tgcgcccctg cggactcaga
     9001 agcagcgata ccgcaccctc gcccggcttc aggccgccga tgttgttcgt caggatggca
     9061 ttcagatagc gaacgcggtc cggcccggtg aacgaaaaat aggcacggta gttcttgtca
     9121 atcagcgcaa cggtttcctt tgcgaagcgg tattccgtct cccactcgcc gaacgcatcc
     9181 ggcagcgcgc acccgaacca cacgcccatc cgcgcgcccg ccgcgcggtg caacttcgcc
     9241 agcggcgtct ccattgtcgg cttcgcagtc tccgtggtca tttgcgcacc cattctatca
     9301 ggttgcagtc cgtggccatt tcctggcgga tcgctcagca aacccgtcat tgtggctgcc
     9361 ctgcttgcac tgtttactct gagcttcgaa gggagcccgc cgcaggcggg tgagcgcaac
     9421 gaaggggact ccgcgcgtgg atggacggga gccgcgtgga agcaaagact ttcttgccgc
     9481 ctagtcctgg cgctccttca cgcgtcgcaa cagatcctgg gccatctttt gatccgcggg
     9541 cgacctggcc gccctcgcca gccgctcggc aatcggacga gcttgttcta cctgattgcc
     9601 gtttagcaga agttggatca ggtgagcggt ataccagagc gtggccggat caagctgtgc
     9661 cgccttggcg gccgcgtcaa gcgcttgatt ccgtgtttct ggaaaacgtg agtacgcgtt
     9721 cgccagcgca tcataggccg gcgcgaatcg cggattgagt tgagtcgctt tcaggaggct
     9781 gtccgtcgct tccttcacgg cgtccgcgtt cccgaagtcc gactggaaaa ggaaaaggcc
     9841 gtgatagtaa ggagcgatga agtccgtcgc gcccagccga agagcctcct gcacctcttt
     9901 gtcggcttct ttaacttccc tgagccggta gtggtagtag cccagactct cgtgtgcgaa
     9961 ggccagattg ggttcgagct tcaccgcctc ttcgagaacc ggtttcgccc tatcgaggcg
    10021 attgtgatgc gcaaaaaagt cgccccgcag ggccaggact tctcccggcg agagattccg
    10081 gaccgcgtag ttcttgcccg tgctctcggc tgaattcttc agaacgctga cgtttaaaga
    10141 cgaccgccgc gcgtaggcgt caatcctccg gccgaactct ttcagatctc caagtgcttc
    10201 ccgggctgca tcgatttggt ttccggactt gtcaaacgcg gaaaggaagc gcttgaggta
    10261 ctgtttttgc cgcgcttcgg ggtccatcaa aaagtaatgc acgacggccc aggattgtgc
    10321 ataaaacacc gatgcgcgat cctgctcgtt gtaatacgga gacccctggt ccacttgaaa
    10381 tagggtttcg atcggcaaga gtttgttctg gtttaggacg tagagctggc ccgggccaaa
    10441 cgtgcccatc gtgatttctt tatcgcccag cgtcgtgttt ccaaaaaact ccgccaagcc
    10501 ttcgttcaac catatcggca ggtcgtggta gttcaaacga agcagggcat gggtgtattc
    10561 gtgatagagc gtgtgatacg gattgtcgcc ctccgaattc aggcgcatga ccacgtaatc
    10621 cttgtcctgg cccggctgat agagccccgc aaggtggacg cgtcccttta cctcccagta
    10681 ttcgggaagg aactccttta tcgtgttctc gttctttgcg gccaggatca ggatcggctg
    10741 gccggcatca acccggaggg tcgtgaaaac gcgctggaac agccctcgaa tctgctcgaa
    10801 ctggtcggca atcttgcgtg cgtctttctc cccggagttg gttgccaccg tgaaatgagg
    10861 acttcgcacc tcgatccagt tgtcggcggc gcttgcaaag cccggaagga gaagaaggac
    10921 aagtgtgaag aaaagcgggc gaatcttcga cattctcacc attggctgta acctcatgta
    10981 cgtacttgtt ttgagaattc cgagcagcgg tatcgtacac cgagttatct tctccgcctg
    11041 cggcctcgcg cgcctcgccc cccaacggcg atgctatact tcagcgcatg aaggaatctt
    11101 ctgcggcccc gcacgcgtcc ggcggggcgc tgattcacga aatcattccc gtgggctggc
    11161 tgcagtgcaa ttgttcgatc atcggcgacc cggccacgcg cgaagcgctg gtgctcgatc
    11221 cgggcgacga ggtcgaaagg atcgaggaaa tcctcaagcg ccaccagctc accgtcaaag
    11281 ccatcgtcag cacgcacgcc cacattgacc acgtcggcgg cttgaaaaag ctgcacgacc
    11341 tgaccggcgc gccggtgatg atgcatgcgg atgatttgca gctctatcag gccctggatc
    11401 gacaggccaa gtggctcggg atgcgcgcgc cggccatgac ggaggttcat cagctcctaa
    11461 aagaaggcga cgcgctgcgc tggggaccct tcgaggctca tgtgatgcac acgcccggtc
    11521 acacacccgg cagcgtatgc ctctatcttc cccgcggcgc cgggaagatc gcactcaaac
    11581 tcccgcatct ttttgccgga gacacgctct tcgcgggcag catcggccgc accgatctct
    11641 ggggcggctc tttcgaagag atcatgcgtt cgctccgcgg aaagctgctc gcgcttccgg
    11701 acgagacact cgttttcccg ggtcacggcc cgaaaacgac catcggggcg gagcgcgatt
    11761 cgaacccgtt tctgctcgtc aaataaatct atcaagtaag attttaagac aaggaagaac
    11821 cacccccgga gccgctcccg gcccgttcga aacatgcctc attgcctctc gccaggggcc
    11881 tgcgaacggc gcaattcttc cattacggac tggatcaact cctttgcgtg ccccagctcc
    11941 tgcgtgatga tctggatgtc catcgccggt acggcggcgt tgcgctggct gcggcaatag
    12001 acgccgtgct ggcccaccag gaccagcgca ttcgttacgc ggtcgagaga gacggccagg
    12061 cgtccgcgcg ccgggccaaa cattcgctcg tatttctcca ggtcctcttg atgctcttca
    12121 aatgccgtca tggccccttc cttgacgctg cgattcaggc ccgccgggcc gtaccactct
    12181 gagcacacga ttttcgctcg caactttacc ccatccggcg cgcggaacat aattcgcagt
    12241 gtcgcaccca tccgcgacac aacctcggaa a
//
LOCUS       DASYVO010000254         5823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_75954, whole genome shotgun sequence.
ACCESSION   DASYVO010000254 DASYVO010000000
VERSION     DASYVO010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75954"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /locus_tag="VGR03_08505"
     CDS             complement(<1..1574)
                     /locus_tag="VGR03_08505"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV1994357.1"
                     /translation="MRRVDLPAAIFLLTAALMLFVPAPGAAQKKDDLPHPKTLPELQQ
                     AMKDVLDKEHVPGAGVAFVANGEVLWCGGIGEADIAEKRAVTCETEFRVGSISKTFVA
                     LALLKLQEEGKINLYARLQDVAPEIPFTNPWEATHPVRIVNLLEHTAGFDDMEFSEVY
                     NLHDRYDFPLLDVFKRFRKPQVVRWPPGTRMSYANPGNAVAGYLIEKVTGKPFDQYIR
                     ETFLRPMGMEKADYPFTNANKPLLAIGYEGNPPKAGGYPFIYLRPAGDLKASPGELVK
                     LVQFLLRRGRAGDTQLVKPESILRMETPETTLAAKNGLRLGYGLCNYSSVEGGVVTHG
                     HDGGIDGFVSSYRYMPEQNWGYVVLLNSTNSGRALHDLDLLAIDFLSRDFPKPQQAAI
                     HPSPSELRGFAGFYASRAPRSQMFAFLDGLAGGTRIRVINGKLTRSGLFSAPEPLLCV
                     GKNVFRGEKEPEGTMIFFTNESGDMAIAGSGLEGIPYSERSSLAMPFIRIALLSSLSV
                     FHADFSSLCTHLALPEA"
     gene            1771..3333
                     /locus_tag="VGR03_08510"
     CDS             1771..3333
                     /locus_tag="VGR03_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994358.1"
                     /translation="MKSPIRFLSSAAVLFLGVNMCSAQQQATPPPDKAAEKPKEEKKP
                     PVPEEKIVQTKHSLKIGGQEIKYTATAGTILLKLEDGTPKASIFYVAYTKDDVSDASQ
                     RPLTFSFNGGPGSASVWLHLGLFGPRRVQMGDAGTLLPPPYKLVDNDVSLLDISDLVF
                     IDPVSTGYSRAVPGEAPKQFHGVTEDVESVADFIRLYATRNKRWSSPKFLAGESYGTT
                     RAAGLSGYLQQRYGMYLNGIVLISAILNFETAEFDTGNDLPYILYLPTYTAIAWYHKK
                     LPSDLQGGDLQKAVEESRKFAVVEYADALMTGDALPAARRTEIMQKLARLTGLSPDYI
                     DRTNLRIEIQRFTKELLRSERRTIGRIDARFLGIDRDAAGEEPEFDPSIAAIIGPYSG
                     MLNDYVRNDLKFDSDLPYEVLTGRVRPWNYAPYENRYVNVAETLRKAMTQNPFLRVFV
                     AKGYYDLATPFFAADYTFDHLGLDPTLRSHLSGAYYEAGHMMYVHPPSLAKLKADIAQ
                     FIKSSTAANPAK"
     gene            3330..5417
                     /locus_tag="VGR03_08515"
     CDS             3330..5417
                     /locus_tag="VGR03_08515"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEV1994359.1"
                     /translation="MKRIFSIVGWVVIAALGAGALGSIALHRGEPLNATWFVVAAACC
                     YLVAYRLYSAFVAAKVLALDDTRATPSERHDDGRDFVPTNKWVLFGHHFAAIAGPGPL
                     VGPTLAAQFGYLPGTLWLIAGAAFAGCVQDFVILFCSIRRDGKSLGQMAREEVSERGG
                     FIAQLAVLAIMTILLAVIGLVVVNALKSSPWATFTLAMTIPIALLLGLYLRFVRPGRV
                     LEASVMGVALVLFVVVAGQWVADSPSWARVFTLSGVALAFAIVAYSFAASALPVWLLL
                     APRDYLSAFIKAGAIFSLAAGILFVRPQVLMPPLTRFIDGTGPVFAGKIFPFCFITIA
                     CGAISGFHSLISSGTTPKMILRESHARFIGYGAMLLESFVGVMAMVAACAMPPGVYFA
                     INSPPSIVGVPGDAAAATISGWGYPLQAQTMTDLAHKVGEITLWNRAGGAPSLAVGMA
                     QIFSSTIGGERLLSIWYHFAIMFEALFILTVLDAGTRVGRFMVQDLGGRVWKPLGRTG
                     WMPGILLSSAVIVALWGYFLYQGVVDPLGGINSLWPLFGISNQLLAAVALVVATTILM
                     KMGRLRWIWVTLLPMAWLVIITMTASYQKIFDANPRIGFLAYAKALAAQVAARKIPAE
                     KIAETQHLIFNQRLDAAVTGVLAAMILVLIVEALAQWYSILSRRKEPVLHESPYVATR
                     WAADYSGAAHGDD"
     gene            5417..5617
                     /locus_tag="VGR03_08520"
     CDS             5417..5617
                     /locus_tag="VGR03_08520"
                     /inference="COORDINATES: protein motif:HMM:NF016234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CstA-like transporter-associated
                     (seleno)protein"
                     /protein_id="HEV1994360.1"
                     /translation="MKHAAKTTSRFLRRFWRGVREWCGDAAYESYLRSKGRQSGSCRT
                     LTPAEFYVEQLNRRYSRPNRCC"
BASE COUNT         1088 a         1806 c         1649 g         1280 t
ORIGIN      
        1 gcttcaggaa gagccaaatg agtgcaaagg gaagagaagt cagcatgaaa aacagacaaa
       61 gaactgagga gagcgatgcg aataaaaggc atggccaggc tgctgcgctc gctgtaagga
      121 attccctcga gtccgctgcc ggcgatggcc atgtcaccgg attcgttcgt aaaaaagatc
      181 atcgtgcctt ctggttcttt ttcgccgcgg aacacattct tgccgacgca gagcagtggt
      241 tcgggcgcgc tgaataatcc ggagcgcgtc aacttgccat tgatgacgcg gatgcgcgtg
      301 cctccggcga gcccgtccag aaacgcgaac atctggcttc ggggtgcgcg cgaagcgtaa
      361 aaaccggcaa acccccgcag ttcgctcggc gacggatgaa ttgctgcctg ctgtggcttg
      421 ggaaaatccc gcgaaaggaa atcaatcgcc aggagatcca agtcgtgcag cgcccggccc
      481 gaattggtgc tgttgagaag gaccacgtag ccccagtttt gctccggcat gtagcgatac
      541 gaggagacaa aaccatcgat gccgccgtcg tggccgtgcg tcaccacgcc gccttccacg
      601 ctcgaatagt tgcagaggcc gtagccaagc cgcaggccgt ttttcgcggc cagcgtggtt
      661 tccggtgttt ccattctgag gatagactcg ggtttcacca gttgcgtgtc tcccgccctt
      721 ccgcgccgca gaagaaattg cactagcttt accaactcgc ctggcgatgc ttttagatct
      781 cccgcaggcc gcaggtagat gaacggatat ccccccgctt tcggcgggtt gccctcgtat
      841 ccgattgcta gcagaggctt gtttgcattc gtgaatgggt agtccgcttt ttccatgccc
      901 atcggccgca gaaacgtctc gcggatgtat tgatcgaagg gctttcccgt gactttttcg
      961 atgaggtagc cggcgaccgc atttccgggg ttcgcataag acatgcgcgt tccgggcggc
     1021 cagcgcacga cttgtggttt cctgaaccgc ttgaaaacgt caagaagagg aaaatcatag
     1081 cgatcgtgca ggttgtaaac ttcgctgaac tccatgtcgt cgaagcccgc cgtgtgttcg
     1141 agcaggttca cgatgcggac gggatgagtt gcctcccagg gattcgtgaa ggggatttcc
     1201 ggcgcgacat cctgcaatcg cgcgtaaaga ttgatctttc cttcttcctg caatttcagc
     1261 agcgccagcg cgacaaatgt cttgctgatc gagcccacgc ggaattccgt ctcgcaggtg
     1321 acggcacgct tctcggcaat gtccgcctcg ccgattccgc cgcaccagag aacctcgcca
     1381 ttcgccacga acgccacacc cgctccggga acgtgttctt tgtcgagcac gtccttcatt
     1441 gcttgctgca attccgggag cgtcttcggg tgcggcagat cgtctttttt ctgcgcggcg
     1501 cccggggccg gaacaaacaa catcagcgcg gcggtgagca ggaaaatagc ggcgggcaaa
     1561 tcaacgcggc gcatgcggcg ctccttggga cacaagttcc atccgagggc cgcattctat
     1621 ccgcgaatcc gcgctatgca aaagaaatcg ctgtagaatc cagccgcggc gcgaggctcc
     1681 aaacgattga cgctgcccgc actctcgcat acgatgccgc acgtcacagt tttgtatttt
     1741 gcgaaggttt gaaagggggc tctgccgcct atgaagtctc caattcgctt tttgagttcc
     1801 gccgcggtgc ttttccttgg agtgaatatg tgctctgccc agcagcaggc aacgccaccg
     1861 ccggataaag ccgcagaaaa gcccaaagaa gaaaagaaac cgcccgttcc tgaagaaaaa
     1921 atcgttcaga ccaagcactc actgaagatt ggcgggcagg agatcaaata caccgcgacg
     1981 gctgggacca tcctgctgaa gctcgaggac ggcacgccca aggccagcat cttctacgtc
     2041 gcctatacca aggatgacgt cagcgacgca agccagcgcc cgcttacctt cagcttcaac
     2101 ggcggtccag gttccgcttc cgtgtggctg catctgggat tattcggtcc tcggcgcgtg
     2161 cagatgggcg atgcgggcac gctccttccg ccgccttaca agctggtcga caacgatgtc
     2221 tcgctcctcg acatcagcga cctcgtcttt atcgatcccg tttcgacggg atacagccgc
     2281 gcggttcccg gcgaggcgcc caagcaattt cacggcgtaa cggaagacgt cgagtccgtc
     2341 gcggatttca tccggcttta tgccacgcgc aacaagcgtt ggtcctcgcc gaaattcctc
     2401 gccggcgaaa gctatggcac gacgcgcgcc gctggcctct ccggatacct ccagcagcgc
     2461 tacggcatgt atctcaacgg catcgtcctg atttcggcga tcctgaattt cgaaacggcg
     2521 gagttcgaca cgggcaatga cctgccgtac atcctatatt tgccgaccta caccgccatt
     2581 gcgtggtatc acaagaaact tccctctgac ttgcagggag gcgatttgca aaaggccgtc
     2641 gaagaatcgc gcaagttcgc cgttgtcgaa tacgcggatg cgctcatgac cggtgacgcg
     2701 ctgcctgctg cccggcgcac ggagatcatg caaaagctcg cccgcctgac cggactttcg
     2761 ccggactaca tcgaccgcac caacctgcgc atcgaaatcc agcgcttcac gaaggagttg
     2821 ctgcgcagcg agcgtcgcac cattggccgc attgatgcgc gcttcctggg catcgatcgc
     2881 gatgccgccg gcgaagagcc cgagttcgat cccagcatcg cggcgattat tggcccctat
     2941 tcgggcatgc tgaatgacta cgtgcgcaac gatttaaaat ttgacagcga cttgccttac
     3001 gaagtgctga cgggccgcgt gcggccgtgg aactacgcgc cctacgaaaa tcgttacgtc
     3061 aatgtggcgg aaacgctgcg caaggccatg acgcaaaatc cgttcctgcg cgtttttgtc
     3121 gccaagggct actacgacct ggccacgccg ttcttcgccg cggactacac cttcgatcac
     3181 ctcggcctcg acccaacgtt gcgaagccat ctcagcggcg cttattacga agccggtcac
     3241 atgatgtacg tccacccgcc ctcgctggct aagctaaagg cggatattgc gcaattcatc
     3301 aaatccagca cggcggcgaa tccggcaaaa tgaagcgcat cttcagcatc gttggttggg
     3361 tggtcatcgc ggctctcggt gccggtgcgc tcggttccat cgccctgcat cgcggcgagc
     3421 cgctcaatgc gacctggttc gtcgtcgctg cggcttgctg ttatctcgtg gcgtaccggc
     3481 tctattccgc cttcgtcgcc gcaaaagttc tcgcgctcga cgacacgcgc gccactccca
     3541 gcgagcgcca cgacgacggc cgtgatttcg tgcccacgaa caaatgggtt ctcttcggcc
     3601 accattttgc cgccatcgcg ggacctggcc cgctcgtcgg cccgacgctt gccgcgcagt
     3661 tcggctactt gccgggcacg ctttggttga ttgccggcgc ggcgttcgcg ggctgcgtgc
     3721 aggattttgt cattcttttt tgctcgattc gccgcgacgg aaaatcgctt ggccagatgg
     3781 cacgcgaaga ggtcagcgaa cgcggcggct tcatcgcgca actggccgtg ctggcgatca
     3841 tgacgattct tcttgccgtg attggcctcg tcgtagtgaa cgcgctcaag tcctctccct
     3901 gggccacctt cacgctggcg atgaccatcc ccatcgcgct gcttctgggc ctctatctgc
     3961 gctttgtccg gcccggccgt gtcctggaag cttcggtcat gggggtggcg ctcgttcttt
     4021 ttgtagtcgt tgcggggcag tgggtggccg attctccttc ctgggctcgc gtattcacgt
     4081 tgagcggtgt cgcgctcgcc tttgccatcg ttgcctacag cttcgcggca tctgcattgc
     4141 cggtctggtt gcttctggcg ccgcgcgact atctcagcgc gtttatcaag gccggcgcca
     4201 ttttttctct tgctgcgggc atcctgtttg tccggccaca agtattaatg ccgccactca
     4261 ctcgcttcat cgacggcacc ggtccggtct ttgccggaaa gattttcccc ttttgcttta
     4321 ttaccattgc gtgcggtgcg atcagcggat tccattcgtt gatctcttcc ggcaccacac
     4381 cgaagatgat ccttcgtgaa agccacgccc gtttcatcgg ctacggcgcg atgctgctcg
     4441 aatcgttcgt cggcgtgatg gccatggtcg ccgcttgcgc gatgccgccg ggcgtctatt
     4501 tcgcgatcaa cagtccgccc agcatcgtcg gagtccccgg ggatgcggct gcggcaacga
     4561 tcagcggctg gggctatccg ttgcaagcgc aaaccatgac cgatctcgct cacaaggtcg
     4621 gcgagatcac cctgtggaat cgcgctggcg gcgcaccttc gctggccgtg ggtatggcgc
     4681 aaattttttc gagcaccatt ggaggcgagc gcctcctcag catctggtat cacttcgcca
     4741 tcatgttcga agcgcttttt attcttacgg tgctcgacgc gggcacgcgc gtggggcgtt
     4801 tcatggttca ggacctcggc ggccgcgtct ggaagcccct cggccgcacc ggctggatgc
     4861 ccggcattct gctgtccagc gccgtgatcg ttgctctgtg gggctacttt ctttatcaag
     4921 gcgttgtcga tccgctcggc ggaatcaatt cgctgtggcc gctctttggc atctccaatc
     4981 aattgctcgc tgcggtcgcg ctcgtggtcg ccaccaccat cctgatgaaa atgggccggc
     5041 tgcgctggat ttgggtgacg ctgctgccga tggcgtggct cgtcattatc accatgaccg
     5101 cgagctacca gaaaatcttc gatgccaatc cgcgcatcgg ttttctggcc tatgccaagg
     5161 ctctcgctgc gcaagtcgcg gccaggaaaa ttcccgccga gaagattgcc gagacgcagc
     5221 acctcatctt taaccagcgt ctcgatgccg cggttaccgg cgttcttgcc gccatgatcc
     5281 tcgttctgat tgtcgaagct ctcgcgcagt ggtactcgat tctcagccgc cgcaaagaac
     5341 ctgtccttca cgaatcgccc tacgtcgcca cgcggtgggc cgcggactac tccggcgctg
     5401 cccacggaga cgactgatga aacacgccgc aaagactacg tcgcgtttcc tgcgccgttt
     5461 ctggcgcggc gttcgcgaat ggtgcggcga tgcggcctac gaaagctacc ttcgttcgaa
     5521 aggccggcag tctggttcgt gccgcacact gacgccggcc gagttctacg tggaacagct
     5581 caaccgccgt tattcgcgcc ccaaccgctg ctgctgaaac tcttatcttg ccgctccggt
     5641 ccgccggtca tacaatccag ttcaactcag tctcactctt ctcaccataa tggaggcagg
     5701 aactgtggcc atcaatcagg acaagctgca cgaatttctt ggcaaagcca tcgtcgattt
     5761 cggcgccacc ttcaatgcgg ctttgattcg tatcggcgac aagctcggct tatataaagc
     5821 cct
//
LOCUS       DASYVO010000255         2723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_260940, whole genome shotgun sequence.
ACCESSION   DASYVO010000255 DASYVO010000000
VERSION     DASYVO010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260940"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..1130)
                     /locus_tag="VGR03_08525"
     CDS             complement(219..1130)
                     /locus_tag="VGR03_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEV1994361.1"
                     /translation="MRATRRGGATADDLAPEQGAAKVAPTIAANEGKKPLRLGLIIGI
                     GRDPHAAMAKVYDLGLPTCQVFVDVTEAELADRLRQALNKYQIEATSLVVGGPGKEVW
                     DFYEGPVTIGLVPRETRDARIAHIKKASDFAKQCGISAVQTHCGFIPENPIDAVYKET
                     VTAMREVAGYCKRNGQDFRYETGQETPITLVRAIQDVGLDNQGVNFDLANLILYGKAN
                     PVDAIELLGPYVQGIHAKDGLWPTNPKELGQEVPIGKGKVEFPQIIARLKEINYRGAV
                     TIEREISGPQQVEDVRAAKAYLEKLIG"
     gene            complement(1257..1889)
                     /locus_tag="VGR03_08530"
     CDS             complement(1257..1889)
                     /locus_tag="VGR03_08530"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEV1994362.1"
                     /translation="MSRKGAFLAAVILLGAAVVAQAPPQTKWVPLFNGKDLTGWKNNG
                     EEKWVVEQGTILCESAANKYGYLTTEKTFRDFNLRLKFKGEAAGNSGVFFHSRITSFD
                     PEHGPDIEGMQVEVDPNVGKHTGGLYESGGRGWVKMPTAEGEQALKPGEWNDLEVSVQ
                     GNHIVTQLNGVKIVDYADTTPRFTDGVIGLQIHTGGGVRVRWKEIFIQQK"
     gene            complement(1997..>2723)
                     /locus_tag="VGR03_08535"
     CDS             complement(1997..>2723)
                     /locus_tag="VGR03_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994363.1"
                     /translation="VLAGAAFLFGLICLAVDLYARRSDRSSWKPFELPLELYVVTFCA
                     TALLPENLRPSIYGGWIGLLGSRLTTISAILGLSFLGYLKARKWHLAGFGVCAMVFFA
                     FLYQDTGWLNRLEANAEKLVGDLPAGTRVIVTIDAPADSRIAFIGHSVERACIAHCFS
                     YANYEPSSGEFRVRVRKGSPVVTSSTDDAEDMASGEYEVDDTDLPMKQVYQCDAKDLT
                     KLCIRDLLAGATNGRLSYKLPSP"
BASE COUNT          539 a          874 c          702 g          608 t
ORIGIN      
        1 cgtccgccgc accccagcag tcccacgcga atcgcggagt ttgccgccgt gccgcgcacc
       61 agctccggct tgatgaacag gattcccgcc gtcgctgccg cagcgccgag gaatttccgg
      121 cggtcaactt tttcggaatt cccgggccgc tccttcttgt cttctgacat acttcctccg
      181 tcctcgtcgt gccgcgcggc ttactgtcaa ttcgacaact atccgatcag cttctcgaga
      241 taggccttcg cggctcgcac gtcttccacc tgctgcggac cggaaatctc ccgctcgatc
      301 gtgaccgcgc cgcgataatt tatctctttc aggcgcgcga tgatctgcgg gaactctacc
      361 ttcccttttc cgatgggaac ttcctgcccg agctccttag ggttcgtcgg ccacagcccg
      421 tccttcgcgt gaatcccctg aacgtacggg cccagcaact caatggcgtc aaccggattg
      481 gccttgccgt acagaatcag attggcgaga tcaaagttaa cgccctgatt gtccaacccc
      541 acgtcctgaa tagcgcggac gagcgtgatg ggtgtctctt gtcccgtctc gtagcggaaa
      601 tcctggccat tgcgtttgca gtatcccgct acctcgcgca tcgccgtaac ggtttccttg
      661 tagaccgcgt caatgggatt ttcggggatg aatccgcagt gcgtttgcac ggcagagatg
      721 ccgcactgct tggcaaagtc cgacgccttt ttaatgtgcg cgatgcgcgc atctcgcgtc
      781 tcgcgcggaa ccagcccaat ggtcaccggg ccctcgtaaa agtcccaaac ttcctttccc
      841 ggtccgccaa caaccagcga agtcgcctca atctggtatt tgttaagcgc ctgacgcagg
      901 cggtccgcaa gctcagcctc ggtgacgtcc acgaacacct ggcatgtcgg caggcccaga
      961 tcgtaaacct tcgccattgc cgcatgaggg tctctgccaa tcccgataat caggccgagc
     1021 ctcagcggct tcttgccttc gttggctgcg atcgttggag caacttttgc tgcgccctgc
     1081 tctggcgcca gatcgtcggc agtagctccg cctctccggg tggcgcgcat tgggctcagc
     1141 gcagccgcca aagctcccgt ggaactttga agaaaggccc gacgattccg tttgctcatg
     1201 agactcactt taaaaggtgc gttcgggatg gtcttggaca agacagccaa tgctctctac
     1261 ttctgctgaa tgaaaatctc cttccacctc accctcacac ctccgccagt gtgaatttgc
     1321 aggccgatta cgccatcagt gaatctcggg gtggtgtcgg cgtagtccac gatcttcacg
     1381 ccgttcagct gcgtcacgat gtgattgccc tgcacggaca cttcgaggtc attccactcg
     1441 ccgggtttca gcgcctgctc gccttccgcc gtcggcatct tcacccagcc gcggcctccg
     1501 ctctcgtaca gaccgccggt gtgcttgccc acgttggggt ccacctccac ttgcatcccc
     1561 tcaatgtcag ggccatgctc cggatcgaag ctggtgatgc gcgagtggaa aaacacgccg
     1621 ctgttgcctg ccgcctcgcc cttgaacttc aggcgcaaat tgaaatcacg aaatgttttt
     1681 tcggtcgtca ggtagccgta cttgttggca gcgctttcgc acagaatcgt tccctgctcc
     1741 acaacccact tctcttcccc gttgttcttc cagcccgtca gatcttttcc attgaaaaga
     1801 ggaacccatt tagtttgtgg cggcgcttgt gccacaactg cggcacctag gagtatcacg
     1861 gcggcgagga aggctccctt tctgctcacg ctaacgcctc catctatggt tcttgaaagt
     1921 tcatagccca tagccggaag gaacttatca caaattcagc agagacccat tctgtttccc
     1981 gctattgccc ctgatttcag ggggagggaa gtttgtagct aagtcggcca ttggtcgccc
     2041 ccgctagcaa gtcgcggata cagagcttcg tcaaatcctt cgcatcgcac tggtaaacct
     2101 gcttcatagg aagatccgtg tcatccacct cgtactcccc ggacgccatg tcttccgcgt
     2161 catccgtcga ggaagtgacc accggacttc cctttcgaac gcgcacgcgg aattcccccg
     2221 aagaaggttc ataattcgca tagctgaagc aatgggcgat gcaggcgcgc tcgaccgaat
     2281 gaccgatgaa tgcgatgcgt gagtccgccg gagcgtcgat ggtcacgatc acccgggtcc
     2341 ccgcgggcag atctcccact aacttttccg cgttcgcttc cagccggttt agccagcccg
     2401 tgtcctgata gaggaacgca aagaaaacca tggcgcaaac gccgaacccg gcgagatgcc
     2461 attttcttgc cttcagatat ccgagaaaac taaggccaag aatggccgaa atcgtcgtaa
     2521 gccttgaacc taataggcca atccatccgc cataaatgga tggccgcaga ttttcgggca
     2581 gcagcgccgt cgcgcaaaac gtcaccacat ataattcgag cggcaattcg aatggtttcc
     2641 aggaagagcg gtcgctccgg cgcgcgtaga gatccacggc caagcaaatg aggccaaaca
     2701 agaatgcggc ccccgccaga acg
//
LOCUS       DASYVO010000256         1816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_163679, whole genome shotgun sequence.
ACCESSION   DASYVO010000256 DASYVO010000000
VERSION     DASYVO010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163679"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1483
                     /locus_tag="VGR03_08540"
     CDS             143..1483
                     /locus_tag="VGR03_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994364.1"
                     /translation="MVLSLFVCAAYSATGQVVRFLRFEEAAETIKLFADAGLSSGDIK
                     DSAGWNDWVRTREAEVRARIDGGVEDSITNLILYGASYTNLPRMESAESAASVTGELT
                     ATARARVHALAAALPNATRNERLRFVSEFLARKGIAGESVEAQLQENLRRLIAEQHAY
                     QEKLKESEAASDPAAKLLARGTLFQERGLSADTSLLPNYALEDTLKTMLRKGAIQPGA
                     IHRILVIGPGLDFTDKRDGYDFYPVQTIQPFAMLEAVARLGLGKAEEISVVTADLNAA
                     VNAHVARLAERGRAGQPYTVQLPRLVSAEWSPEAVAYWQKFGEILGTPVKPLPVPASV
                     SDVTMRAVAIRPRYAARIRGFDMNVVTQTMDVPEGQGFDLVVATNVLVYYDLFQQALA
                     MGSIAHMMNHGGIFLANHALPAQHVQALEFLGRRTVAYTPSGAYGDDVVVYRRR"
BASE COUNT          358 a          479 c          594 g          385 t
ORIGIN      
        1 cctgaaggtc tgagcccggg gactcgttgg ggcgatattg aattgactcg gtccgacgct
       61 tggccctaag atgcgcggaa cgatgcaggt tgcccgggaa ttcctatcct ctgccaaagc
      121 taggcgctct gccctcgctt gtttggtcct ctctctattc gtttgtgcag cttattcggc
      181 gacaggacag gttgtccgat ttctccgctt tgaagaagcg gcagagacga tcaagctgtt
      241 tgcggatgcg ggcctcagca gcggcgatat caaggattct gctgggtgga acgattgggt
      301 ccgtacgcgc gaagcggagg tgcgggcgcg catcgacggt ggggtggaag attccatcac
      361 caatctgatt ctttatggcg cttcctacac gaatcttccc cggatggaaa gtgcggagag
      421 tgcggcgtcg gtgaccggag aattgacggc gaccgcccgc gcgcgagtgc atgcactggc
      481 ggcggccctg ccgaatgcga cgcgcaacga gaggctgcgg ttcgtcagcg agttcttggc
      541 gcgcaagggc atcgctggag aatctgtcga agcgcaactc caagaaaatc tgcggcggtt
      601 gattgccgaa cagcatgcgt atcaggaaaa gctgaaggaa tctgaagcag caagcgatcc
      661 ggctgcgaag ttgctggcgc gaggaactct ttttcaagag cggggattgt ctgccgatac
      721 ttccttgctg ccgaattacg cgctggaaga tacgctaaaa acgatgttgc gaaaaggagc
      781 aatccagccg ggcgcgattc accggattct ggtgatcggg ccggggttgg attttaccga
      841 caagcgcgac ggctacgatt tttatccggt gcaaacgatt cagcccttcg ccatgctgga
      901 agcggtggcg cggctcggcc tgggcaaagc cgaagaaatt tcggtcgtga cagcggattt
      961 gaacgcggca gtcaacgcgc acgttgccag gctcgccgaa cgtgggcgag cgggccaacc
     1021 gtacacggta cagctgccgc ggctcgtctc cgcggagtgg agcccggagg cagtggccta
     1081 ctggcagaag ttcggggaga tcctgggcac gccggtgaaa ccgctgcccg ttcctgcttc
     1141 ggtaagcgat gtgacaatgc gcgccgtggc gatccggccg cgatatgccg cccgaatccg
     1201 gggattcgac atgaatgtgg taacgcagac gatggacgtt cctgaaggac agggattcga
     1261 cctggtggta gcaaccaacg tgctcgtcta ctacgacctt tttcagcagg cgctggcgat
     1321 gggcagcatc gcgcacatga tgaaccacgg aggcattttc cttgcgaacc atgcgttgcc
     1381 cgcgcagcac gtgcaggcat tggaatttct gggccggcgg accgtagcct acacgcccag
     1441 cggagcgtat ggcgatgacg tcgtggtgta ccggcggcgg tgaagttcgg cgtggttaac
     1501 aaagtccccc atattcctcg acttgctggc agaatcgcag ttgagttgtg tttcgcacag
     1561 gctaaagcct gtgccactgg tgactcggga cagacaccct ttccgacagg gtcggaacct
     1621 tcgatcattg ggtgtcaccc ctcccctggt tttttgtaag tgttgcatct aaaggactta
     1681 gagtggctct gttgacatgc tcggcacagt gtgcgttttg aggggtcgac aagcggaggg
     1741 ggagcgtgtt ttctgatcaa gcaggaggga cgatggccga gatcggactc ctgctttttg
     1801 ctcgcgtggc cttgca
//
LOCUS       DASYVO010000257         3086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_50757, whole genome shotgun sequence.
ACCESSION   DASYVO010000257 DASYVO010000000
VERSION     DASYVO010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50757"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /gene="hslV"
                     /locus_tag="VGR03_08545"
     CDS             <1..425
                     /gene="hslV"
                     /locus_tag="VGR03_08545"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEV1994365.1"
                     /translation="RKTRRLYNDKVVAGFAGSTADAITLFERFESKLQEFSGNLQKAA
                     VELAKEWRKDRALRHLEALLIVADTKSTFVLSGNGDVIEPDDGLVAIGSGGTFALSAA
                     RALVKHSKLNAKDLAMEAMRIASEICIFTNANFTVEEL"
     gene            690..2129
                     /gene="hslU"
                     /locus_tag="VGR03_08550"
     CDS             690..2129
                     /gene="hslU"
                     /locus_tag="VGR03_08550"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003740291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEV1994366.1"
                     /translation="MTIKSEKELVLVPGGNGAEPVEGQELLPPLDDMTPREIVAELDK
                     YIVGQNAAKRAVAVALRNRVRRQKLPPEIAEDVLPKNILMIGPTGVGKTEIARRLARL
                     AGCPFVKVEASKYTEVGYVGRDVESMVRDLVETSIDMIREEKLDEVADRAEQAAEERV
                     LDLLLPPAPPPPPGTPEAEVAAQREQTQRTREKLRLQLREGKLDQRMVDLEVRERAMP
                     PFEIISNQGVEEMDVNIKDMLSGMFGQQKKKRKMSVADAFEYLIQEEENKLLDMDQIT
                     RVAVERAEQMGIIFIDEIDKVAGRESGHGPDVSREGVQRDILPIIEGTTVNTRYGMIR
                     TDHILFIAAGAFHVSKPSDLIPELQGRLPIRVELQSLKEEDFIRILTEPKNALIKQYV
                     ALLETEGVKLNITEDAVAAVARFATSVNEQTENIGARRLHTILEKVLDEISFEAPDLK
                     KKNVKVDAAYVTKQLADIVKNQDLSRYIL"
     gene            2146..>3086
                     /locus_tag="VGR03_08555"
     CDS             2146..>3086
                     /locus_tag="VGR03_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994367.1"
                     /translation="MENRKGTVLHITKRPAQVFAIFYFLFSIILFSAGCGAPGDPVPP
                     SPPVPVAIKDLSAHQAGDGVQLSFTLPTNSISGEKLPAPPAIEILRGAVKPDGSADAK
                     SFRVVYTIPGALVESYRADGRVRFTDPIAPEETKSQHGGAVAYIVRTRASQKRASADS
                     NAVSLSVFSVPTPIASIDARVTESAVELTWPVPAGTAAGEPASSITGYRIYRSEIHPS
                     APASSPQDLPAGKPESHAALLASSETNSYRDGSVVFDHSYVYIVRSVIQVGGNELESS
                     DSQPVTVTPRDTFPPAAPQSLVAALLPGSAAGTVLVDL"
BASE COUNT          672 a          969 c          861 g          584 t
ORIGIN      
        1 cgcgcaagac gcgccgcctt tacaacgaca aggtcgttgc tggttttgcc ggctccacgg
       61 ccgacgcgat cacacttttt gaacgattcg aaagcaagct tcaggagttt tccggcaatc
      121 tccagaaagc cgcggtcgag ctggccaagg aatggcgcaa agaccgcgct cttcgccatt
      181 tggaagccct gctgatcgtt gccgacacca agagcacttt cgttctttcc ggcaacggcg
      241 acgtcattga gccggacgac ggtctggtgg ccattggttc tggcggaact ttcgcgctct
      301 ctgccgctcg cgcgcttgtg aagcattcga aactgaatgc caaggatctg gccatggagg
      361 ccatgcgcat cgccagcgag atttgcattt tcaccaacgc caactttacg gtcgaagagc
      421 tctagtccgg agttccaacc agcggacggg tgtattttag gcgaagcggg cagacagcgg
      481 cggaaagacg gttgatcagg cgagcaggaa cgacatggtg tggcgagggc aagaagagcg
      541 gcccgcctcc ccgacaagag tcggggtaaa cgccaggcgg gccctacaag gaaccagcgg
      601 acgggccaaa ttcttgtgag aaatacgtac cagaagtagc gtgcaatcac gccaagctat
      661 tttcgcgcat caaaggaact gagcaagtca tgacgataaa gtccgaaaaa gaattagtcc
      721 tcgtaccggg aggcaacggc gccgagccgg tcgaggggca ggaacttctt ccccctctcg
      781 atgacatgac gccgcgcgaa atcgtggccg agctcgacaa gtacatcgtc gggcagaatg
      841 ccgccaagcg cgccgtcgct gtggccttgc gcaaccgcgt ccgccgccag aagcttccgc
      901 cggaaatcgc cgaagacgtt ctgcccaaaa acattctgat gatcggcccc accggcgtcg
      961 gaaaaacaga aatcgcgcgc cgactggcgc gcctcgccgg ctgcccgttc gtcaaggtcg
     1021 aagcttcgaa atacacggaa gtcggctacg tcggccgcga tgtggaatcg atggtccgcg
     1081 atctcgtgga gacctccatc gacatgatcc gcgaggaaaa actcgacgaa gttgccgacc
     1141 gcgcggagca ggccgcggaa gaacgcgtac tcgatctcct gcttccgccg gctcctcctc
     1201 cccctcccgg cactccggaa gccgaggttg ccgcgcagcg ggaacagacg cagcgcactc
     1261 gcgaaaagct gcgcctccaa ctgcgcgaag gcaagctcga tcaacgcatg gtggacctgg
     1321 aagtccgcga gcgcgccatg cccccgttcg aaatcatctc caatcagggc gtcgaggaga
     1381 tggacgtcaa tatcaaggac atgctctccg gcatgttcgg ccagcagaag aaaaagcgca
     1441 aaatgagcgt cgcggacgct ttcgaatact tgatccagga agaagaaaac aagctgctgg
     1501 acatggacca gatcacgcgc gtggccgtgg agcgcgccga gcagatgggc atcatcttca
     1561 tcgatgagat cgataaggtt gccggccgcg aatccggcca cgggcccgac gtttcgcgcg
     1621 aaggcgtgca gcgcgacatt ctacccatta tcgaaggcac caccgtcaac acgcgttacg
     1681 gtatgattcg caccgatcac attcttttca tcgccgcggg cgcgtttcac gtgagcaagc
     1741 cgtccgatct gatccctgaa cttcagggcc ggctgcccat tcgcgtcgag ctacaatccc
     1801 ttaaggaaga agattttatt cgcattctga ccgagccaaa gaatgcgctc atcaagcagt
     1861 acgtggcgct gctcgaaacc gaaggcgtca agctcaacat caccgaagac gcggtcgccg
     1921 ccgtcgcccg cttcgccacc agcgtgaacg agcagaccga aaacatcggc gcccgccgcc
     1981 tccacaccat cctcgaaaaa gttctcgacg aaatctcgtt cgaagcgccc gacctcaaga
     2041 agaagaacgt gaaggttgac gctgcttacg tcaccaagca gcttgcggac atcgtcaaga
     2101 accaggatct gagccgctac atcctgtagt tcctgaaaat ggaaaatgga aaatagaaaa
     2161 gggacggtac ttcacattac aaaacgcccc gcgcaagtct ttgccatctt ctattttctc
     2221 ttttccatta tcttattttc ggccggctgt ggcgctcccg gcgatcccgt tccgccttcg
     2281 ccgccggttc ccgtggcgat caaggaccta tccgcacacc aggctggcga cggcgtgcaa
     2341 ctttccttca ccctaccgac gaactccatc tccggggaaa aactcccggc gccgcctgcg
     2401 atcgaaatcc tacgtggcgc cgttaaacct gacggctcgg ccgacgcgaa gtctttccgg
     2461 gttgtttaca cgattcccgg cgctctggtg gagagttacc gcgccgatgg ccgcgtgcgc
     2521 ttcaccgatc ccatcgcgcc ggaagaaacc aaatcgcagc acggcggcgc ggtcgcatac
     2581 atcgttcgaa cgcgcgcttc gcagaaacgc gcttccgccg attcgaacgc cgtctcgctg
     2641 agcgtttttt cggttccgac tcctatcgct tccatcgatg ctcgcgtcac ggagtcggcg
     2701 gtcgaattga cctggccggt gccggccggt actgccgccg gggagcctgc atcaagcatc
     2761 accggttatc gaatctaccg ttcggaaatt catccttccg ctcccgcttc atcaccgcag
     2821 gatcttccgg cgggcaaacc ggaatcgcac gctgcgctgc tcgcttcttc tgaaactaac
     2881 agctaccgcg acggttccgt tgtgttcgac catagctacg tctacatcgt gcgcagtgtc
     2941 atccaagttg gaggcaacga gctggaatct tctgactccc agccggtgac ggtaactccc
     3001 cgcgacactt ttcctcctgc cgctccgcag agcctcgtgg ccgcgttgct tccgggttcg
     3061 gctgcgggca cggttcttgt tgacct
//
LOCUS       DASYVO010000258        10161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_50809, whole genome shotgun sequence.
ACCESSION   DASYVO010000258 DASYVO010000000
VERSION     DASYVO010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 10161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10161
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50809"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VGR03_08560"
     CDS             <1..958
                     /locus_tag="VGR03_08560"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEV1994368.1"
                     /translation="TTVNRLCGSSMDAVASAARAIKCGETDLMIAGGVESMSRAPFVV
                     AKADSAYSRSMKMEDTTLGWRFVNPLMKAKYGVDSMPETGENVAEEFRISRRDQDLFA
                     LRSQQRAAEAIRSGRLKQEIVAVPIPQKKGDPLLFSEDEHPRPDTRLEALSKLKPVVK
                     PNGTITAGNASGVNDGACALLLASEAALKRHALTPRARVVAAAAAGVAPRIMGMGPTP
                     ATRKVLAKAGLSLGQMDVVELNEAFASQALAVLRELGIPDDSAHVNPNGGAIALGHPL
                     GASGARLVTTALYQLLRTNGRYALCTMCIGVGQGIALIVERI"
     gene            1067..2425
                     /locus_tag="VGR03_08565"
     CDS             1067..2425
                     /locus_tag="VGR03_08565"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006554.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HEV1994369.1"
                     /translation="MNHLDPLFRSPAVDNLFTDRATLQGMLDFEVALARAEARIGVIP
                     AAAAAAIATKCRAELFDTAALAQAAARAGNIAIPLVKHLTALVTKKDKQAARFVHWGA
                     TSQDVIDTGRILQLRQALDLIARDLDSLAQPLGSLAQKHRSTLITGRTWMQHALPTTL
                     GVKIAGWLDAIVRNRARLTETRKRCLVLQFGGAVGTLAALGTRGPDVASLLAAELGLG
                     LPDLPWHGHRDRIAEIGVTLGLCAGTVGKIARDLSLHMQTEIAEIFEPAGEGRGGSST
                     MPHKRNPVSSAVMLQAAIRVPGLVSTLLTAMMQEDERGLGGWHAEWETLPDIVQLTAG
                     AIHHLATIVPNLEVDVQRMRKNLDLTQGLIFAEAVTMALGEKIGRAQAHEIVEAACAG
                     ARKEKRDLRTILSADPEIEAQLSTADLDRLFDPRNYLGAAEEYVYRVVAASRGKIPAG
                     EE"
     gene            2431..3210
                     /gene="pcaD"
                     /locus_tag="VGR03_08570"
     CDS             2431..3210
                     /gene="pcaD"
                     /locus_tag="VGR03_08570"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR02427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HEV1994370.1"
                     /translation="MPIIKSGEARIHYELEGQCGSSVLVFSNSLGTNYSMWDPQAREF
                     RKKFRVLRHDTRGSGQSSLTLGPYSIEQLAKDVLALLDALDLDRVHFCGLSMGGMIGM
                     WLGVNAPERLNKLVLCNTAARIGTPETWNARIDAVQKNGMKSIAPAVVERWFSPVFRQ
                     NAPATISSTQKMIEETNSDGYSACCAAVRDFDFREQVSRIRVPALVIAGALDPATPPA
                     DGRFLAHQVPGARYVELNAAHLSNIEDQDRFNNEVAAFLNS"
     gene            3223..3603
                     /gene="pcaC"
                     /locus_tag="VGR03_08575"
     CDS             3223..3603
                     /gene="pcaC"
                     /locus_tag="VGR03_08575"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR02425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HEV1994371.1"
                     /translation="MDERQRYELGMKVRRAVLGDAHVDASLKNRNEFNEEFQDLITRY
                     AWGEIWSRPGLPRQTRSMLTLAMMVALNRPDELRMHLRAALNNGVTREEIREVLLQTA
                     IYCGVPAANSAFHLAQEVFEQQEK"
     gene            complement(3712..4065)
                     /locus_tag="VGR03_08580"
     CDS             complement(3712..4065)
                     /locus_tag="VGR03_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994372.1"
                     /translation="MKSTRRGFSTGNKNKWLPAMQAMSYQNIAGGLADEMMSFIRPSE
                     PRKCSPPFKLMIIDRSGTVVFECKVGKDGNVRPFGLARRVRDSHFPATALLTDRSLLT
                     RTFLIERATPSDSNN"
     gene            4430..5152
                     /locus_tag="VGR03_08585"
     CDS             4430..5152
                     /locus_tag="VGR03_08585"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1994373.1"
                     /translation="MPPKNRNGLPAYQRIQNAIRKRIDAGQLHPGDAVASERDLAKIH
                     QVSLMTARHALASLEREGIVERRRGIGTFVAAPKIHFNKLMSYTEQMASRSLTAGSKV
                     LFARVLENENEAAARLSLPPTSSIIKLERLRHAAGEPFALETCYLNAAEFSGLLDAPI
                     GRESLFGILERNYKIELGYADEEIDATAADPRMAEILSIPRRDPLLRIRQVIYSTKGR
                     AIMYVLGFYRSDRHNLVIRRFR"
     gene            5227..6021
                     /gene="nagB"
                     /locus_tag="VGR03_08590"
     CDS             5227..6021
                     /gene="nagB"
                     /locus_tag="VGR03_08590"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV1994374.1"
                     /translation="MLVILKRDDEEISLQAAQLIASAVHKKPALALGLATGSTMVGVY
                     KHLVGLHKQGSLDFSRVITFNLDEYFGLSPAHPQSFHHFMQQNLFAHVNVNPRNIHIP
                     DGTIGGNYDRYCASYEEAIRKAGGIDLQLLGIGRNGHVGFNEPTSSLGSRTRLKVLSQ
                     ETMDDNSKFFALGEESPRCAITMGIGTILEARKILLLAAGAAKSAAVAKSIEGPIASA
                     VSGSALQLHPDVTFIIDDAAASQLTQRDYYRRVLEMTALLTPDRLS"
     gene            6127..6744
                     /locus_tag="VGR03_08595"
     CDS             6127..6744
                     /locus_tag="VGR03_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HEV1994375.1"
                     /translation="MKPAKLFPMFLKLSARPCLVVGAGTIAESKIASLLEAGGRVRVV
                     APEATPQVRSWAQSNSIEWLQRPFRPDDLEGMFLVVAATSSTQLHERIFEEATQRAVL
                     CNIVDVPPLCDFYYPSVVQRGVLQIAISTAGQSPALAQRLRKQLEDQFGPEYEEWLAQ
                     LGETRDQLHSSKLDPEERKRLLHEDASEEAFEAFLRNRRPSKRSG"
     gene            6957..7337
                     /locus_tag="VGR03_08600"
     CDS             6957..7337
                     /locus_tag="VGR03_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV1994376.1"
                     /translation="MKGKVYLMRAGPGDPDLLTVKAIRVLRAAEVVLHDDLVSSEILN
                     LIPASTQVRNVHKLGLQVGNLQEKIHSLLIAAARQGHQVVRLKANDPLAVEETEVLAQ
                     AGVDFEVIPGAASAMGAAVGANSR"
     gene            complement(7373..8242)
                     /locus_tag="VGR03_08605"
     CDS             complement(7373..8242)
                     /locus_tag="VGR03_08605"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEV1994377.1"
                     /translation="MSGFAGLRVLALESRRAPELAKLISNYGGEPIVAPAMREVPLES
                     NREALAFAAALFAGEFDMVIFLTGVGARALLSVVETTYKREEYVAALQRVSVVPRGPK
                     PVAVLRELGVTPAITVPEPNTWRELLLALDEAGASRAELRLRGARVAVQEYGVSNPEL
                     LSGLRERGASVTRVPIYQWALPEDLAPLQAAVRALAEGEIDVVLFTTGVQVAHLFQIA
                     AEMKLEKLMLRGVNRAIVASIGPTTSEELQRRGIRADLEPSHPKMGFLVKEAAEQSEG
                     LFRRKRQRNGSAD"
     gene            complement(8300..8713)
                     /gene="nirD"
                     /locus_tag="VGR03_08610"
     CDS             complement(8300..8713)
                     /gene="nirD"
                     /locus_tag="VGR03_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HEV1994378.1"
                     /translation="MSNKRWIRITAMQNIPLREGRAVNIAGREIAIFNLGDRVLAVDG
                     RCPHKGGPLAEGIVTGNAVVCPLHAWKISLETGSVTKPESEPACVATYRTRIESGIVL
                     IEVPESCTEKQGNAISCGEQNRAASGDSPVPSPAL"
     gene            complement(8710..>10161)
                     /locus_tag="VGR03_08615"
     CDS             complement(8710..>10161)
                     /locus_tag="VGR03_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV1994379.1"
                     /translation="MGVDVFSAGNFNEKVPGTDAVRYEDPALGIYKKLTVRDGKLAGV
                     ILVGDTSDSHRYMDWLRASTDLTAMRRQLFFPGPVEDTGLDVAEMSDSKVVCGCNGVT
                     KGAIIQAVHEKGISTLAQLKDCTRASTGCGSCTEVCQHLLKAVAPEFQEEAKKSLCKC
                     LPFPEDKLREILRSQRLRSVQEVLEIYGNGVGCEICKPALSYMVDMLWCGDHDEDRSA
                     RFINDRVHANIQKDGTFSVVPRIRGGVTSAKELRRIADVAEKYNVPMVKITGSQRIDL
                     LGVKKADLPQIWADLGMPSGQAYTKGVRMVKTCVGTDFCRFGVQDSTKAGIELERRLE
                     HLFTPHKLKMGAVGCPRNCAEATVKDIGLVGQEGSWQVVVGGAAGKKVRKADLLITVE
                     STEEALQAATLFFQYYRENANYLERTYDFVERWSIEKVRRETVYAPLAIQQGLRERLR
                     KSKRISRDAWLEGDAPRHPTQFVQIEPLEKVQA"
BASE COUNT         2102 a         3158 c         2784 g         2117 t
ORIGIN      
        1 cacgaccgtt aaccggctct gcggctcgag catggacgct gttgcctccg cagcgcgggc
       61 catcaaatgc ggcgagacgg atctcatgat tgccggaggc gtcgagagca tgtcgcgagc
      121 gcccttcgtt gtcgccaagg ccgacagcgc ctattcgcgc tccatgaaaa tggaagacac
      181 cacgctcggt tggcgcttcg taaatcccct gatgaaagca aaatatggcg ttgattcgat
      241 gccggagacc ggcgaaaacg tcgccgaaga atttcgcatc tcgcgccgtg atcaggatct
      301 gttcgcgctc cggagccagc aacgcgccgc cgaggccatc cggagcggca ggctgaagca
      361 agaaattgtg gccgtgccca tccctcaaaa gaagggtgac ccgctccttt tttctgagga
      421 tgagcatccc cggccggaca ccaggctgga agcgctttcg aaattgaaac cggttgtgaa
      481 accgaatggc acgatcaccg ccggaaatgc ttcaggagtg aacgacggtg cttgtgcatt
      541 gctgttggcc tcagaggcag cgctgaaacg tcatgcgctg acgcccagag cccgggtcgt
      601 tgctgcggca gcggctggcg tcgctccgcg aatcatgggc atgggtccga cgcccgcgac
      661 acgaaaagtg ctggccaaag cgggattgtc actcgggcaa atggatgtcg tggaattgaa
      721 tgaagccttc gcgtcacagg cgctggcggt gctgcgcgaa ttgggaattc cggacgactc
      781 ggcgcacgtg aatccgaacg gtggtgcgat tgctctcggg caccccttgg gtgctagcgg
      841 cgcgcggctc gtaaccacgg cgctgtatca gctcttgcga accaatggta gatacgcgct
      901 atgtaccatg tgcatcggtg tgggccaagg aattgccctg atagtggaac gcatctaact
      961 tgacctgcga tctacaacgc tcacagttcc ttcaagcagg agatgtttcg tcagaggcgc
     1021 gaggaaaccg ttttctttgt ctgccaggaa tgattaaact catcgcatga atcatctcga
     1081 tcccctcttc cgctcaccag cggttgacaa cctgttcacg gatcgcgcca cgctccaggg
     1141 aatgctcgat ttcgaagtgg cgctggcacg cgctgaagcg cgcatcgggg ttatacccgc
     1201 agcagcggct gccgcgattg ctacgaagtg tcgggcggag cttttcgata ctgccgcgct
     1261 ggcacaggcc gctgctcgtg ctggaaatat tgccattccc ctcgtaaagc acctcacggc
     1321 actcgtgacg aaaaaagaca agcaggcggc ccgcttcgtc cactggggcg caaccagcca
     1381 ggacgtcatc gacaccggcc gcatacttca actacgccag gctttggact tgatcgcgcg
     1441 cgatttggac agcctggcac agccgctggg ctcgctcgcg cagaaacatc gctccacgct
     1501 catcaccggc cgcacctgga tgcaacacgc tttgccgacg acgctcggcg tcaagatcgc
     1561 gggctggctg gatgccattg tacggaatcg cgcgcgcctg accgagacga gaaagcgctg
     1621 cttggtgctc cagttcggcg gcgccgtggg aacattggcc gcgctcggga cccgcggtcc
     1681 ggacgttgcg agcctcctcg ccgcagagct tgggctcggc ctgcccgatc tgccctggca
     1741 cggccaccgg gatcgcatcg cggaaatcgg tgtcaccctc ggcttgtgcg ccggaacagt
     1801 aggaaaaatt gcccgggacc tttccttgca catgcaaacc gaaatcgcag aaatctttga
     1861 gcccgccggc gaaggccgcg gcggctcttc caccatgccg cacaagcgca atccggtaag
     1921 ttccgcggtg atgctgcaag cagccatccg cgttcccgga ctcgtcagca ctctgctcac
     1981 ggcgatgatg caagaggacg aacgcggcct gggcggttgg cacgccgagt gggagacctt
     2041 gccggatatc gtccaattga cggccggcgc tattcatcac ctggccacaa tagtgccgaa
     2101 cctcgaagtc gatgtgcagc gcatgcgcaa gaatctcgat ctgacgcaag ggttgatctt
     2161 cgcggaagcg gtaactatgg ctcttggcga gaaaatcggc agagcccagg ctcatgaaat
     2221 cgtcgaggca gcatgcgcag gagcgcgcaa ggagaagcgt gacttgcgga cgattctgag
     2281 cgccgaccca gagatcgaag cacaactttc gaccgctgat ctggatcgct tgttcgatcc
     2341 gcgcaactac ctgggagccg cggaagaata tgtctaccgc gtcgtcgccg ccagccgggg
     2401 caaaattcct gcaggggaag agtaaccacc atgcccatca tcaagtccgg agaagcccgc
     2461 attcattacg agttggaagg ccagtgtgga tcgtccgtgc tggtattctc gaattcgctc
     2521 ggcacaaatt attccatgtg ggatccacaa gcgcgcgagt ttcgaaagaa gtttcgcgtc
     2581 ctgcgccatg acacgagggg tagtggacaa tcttcgctca cgctggggcc ctactcgatc
     2641 gagcagctcg cgaaagatgt cctcgcactg ctggatgcat tggacttgga ccgtgtccat
     2701 ttttgcggtc tatccatggg tggaatgatt ggcatgtggc tgggagtcaa cgcgccagaa
     2761 cgcctcaata agctggttct ctgtaacact gcggcaagga ttggaactcc agagacttgg
     2821 aacgcccgta tcgacgcggt tcaaaaaaac ggcatgaaat cgatcgctcc agcggtcgtc
     2881 gagcgctggt tttcgcctgt tttccgccag aacgcgcccg caacgatatc aagtactcaa
     2941 aaaatgatcg aggaaacaaa ctcggatggc tattcggcgt gttgcgccgc cgttcgcgat
     3001 ttcgatttcc gcgaacaagt gagcaggatt cgcgtgcctg ccctggtgat cgctggcgcg
     3061 ctcgatccgg ctacgccccc tgcggacgga cgctttctcg cgcatcaggt tccgggcgcg
     3121 cgatatgttg aacttaacgc cgcgcacctt tccaatatcg aagatcaaga ccgtttcaat
     3181 aatgaagttg ccgcgtttct gaactcttga ggaggcccgc cgatggacga gcgccagcgc
     3241 tacgagctag gaatgaaggt gcgccgtgcc gtgctcggcg atgcgcacgt ggatgcttcg
     3301 ctgaaaaatc gcaatgaatt caacgaagag tttcaggacc tgataacgcg ctacgcttgg
     3361 ggagaaatct ggagccgccc cggccttccc cggcagacgc gcagcatgct cacgctggcg
     3421 atgatggtgg cgctgaatcg gcctgacgag cttcgcatgc acttgcgcgc ggcgttgaac
     3481 aacggcgtga cccgcgaaga aatccgcgag gttctcctgc aaacggctat ttattgcggt
     3541 gtgcccgcag cgaactcggc atttcatctc gcgcaggaag tatttgaaca gcaagagaag
     3601 tagaccttct agttcgcatc ctgcgcaatc aatgaggagt gacagcctga atcctacatc
     3661 agcttggcaa gacgcttccc agcggaaacg tgcttcgcaa cgtgacgacc atcagttgtt
     3721 gctgtcactg ggcgtcgcgc gctcaatgag gaatgtgcgc gtgagcagcg aacggtcagt
     3781 aagcaatgca gtcgccggaa aatgagagtc ccttaccctg cgcgccaatc cgaagggccg
     3841 cacgttcccg tccttaccga ctttgcattc gaatacgacc gtgccgctcc tgtcgatgat
     3901 catcagtttg aacgggggac tacatttgcg gggttcgctt ggccggatga agctcatcat
     3961 ttcgtcagcc agtccgccgg ctatgttctg gtagctcatg gcctgcattg ctggcagcca
     4021 tttgttcttg ttgcccgtgc tgaatccacg cctggtgctt ttcatttctt gatctctctt
     4081 tcaggtattg cggtcgactt ggaagaagct cgatcttgga tccaagcagg ccgccctgga
     4141 aaacccaagg cgcgcatcca acgccccaag ccagctatat ggccgaactg tttccagcta
     4201 tatagcacca tttttcgatt gtcaagcggt atttgatgtt ttataaagat gtacggagat
     4261 aggactatag cacttttggg tttccgctct ataacactat agttcctgga atgaactctc
     4321 cccgttttcg ggccttgacc taaaatcaga atagaatatc gttcttgtca tgaagccagc
     4381 ttccacccag gtgcccacca agaagccccg ggccaattcg ggagacagaa tgccacccaa
     4441 gaaccgcaac ggcttgcctg cctatcagag aattcagaac gccatccgca aacgcatcga
     4501 tgccggacaa ttgcatcctg gggacgccgt cgcctccgaa cgcgatctcg ccaagattca
     4561 tcaggtcagc ctcatgaccg cacgccacgc tcttgcctcg ctcgaacgcg aaggaatcgt
     4621 cgaacgccgc cgcggcatcg gaacgtttgt tgccgctccc aaaattcact tcaataaatt
     4681 gatgagctat accgagcaga tggccagccg cagcctcacc gccggctcca aggtgctttt
     4741 cgcgagagtg ctggagaatg aaaacgaagc tgcggcccgg ctctctcttc cccccacgag
     4801 cagcatcatc aagcttgagc gattgcgcca tgccgcgggt gagccctttg ctctagagac
     4861 ctgctacctc aacgctgctg aattctcagg gcttttggac gcccccatcg gccgggaatc
     4921 tctgttcggc attctggagc gcaattacaa gatcgaactc ggctatgctg acgaagagat
     4981 tgacgcgacc gcggccgacc cgagaatggc ggagatcctc tccattcccc gccgcgatcc
     5041 cctgctgcgc attcgccagg tcatctattc caccaaaggc cgggcgatca tgtatgtgct
     5101 gggcttctat cgatcggacc ggcacaatct cgtcattcgc cggttccggt aacgctgacg
     5161 gatttctttc gctggcccag tattgtttca ggtaatggga aaaacaaagt tctgagagga
     5221 agccgcatgc tggttatcct gaagcgcgac gacgaagaaa tcagcctcca ggccgcacaa
     5281 ctcatcgcca gcgcggtgca caagaagcct gcattggcgc tcgggttggc cacgggcagc
     5341 actatggtcg gcgtctacaa acatctcgtg ggactgcaca aacagggctc gctcgatttc
     5401 tcacgcgtca taacctttaa tctcgatgag tatttcggac tttcccccgc acaccctcag
     5461 agctttcatc atttcatgca gcaaaacctt ttcgcgcacg tcaacgtgaa tccccgcaac
     5521 atccacatcc ccgatggaac catcggcggc aattacgatc ggtactgcgc atcctatgaa
     5581 gaagccatcc gaaaagccgg agggatcgac cttcagcttc tgggcatcgg gcgcaatggc
     5641 cacgtcggct tcaacgagcc gacttccagc ctcggctccc gcacgcgcct gaaggtcttg
     5701 agccaggaaa caatggacga caactccaaa tttttcgccc ttggcgagga gagtccgcgc
     5761 tgtgccatca ccatgggcat cggcactatc ttggaggcac ggaaaattct cttgctcgcc
     5821 gcgggcgcgg ccaagtccgc ggctgtcgcg aagtccatcg aagggccgat cgcctccgcg
     5881 gtctccggct ccgccctgca actccatccc gacgtgactt tcatcatcga cgatgccgcg
     5941 gcttcgcaac tcacccagcg cgactactat cgccgcgtcc tcgaaatgac cgctctcttg
     6001 actcccgatc gtctctctta aatccaaatt ccccacgctc tcggcttgac cctgagtaca
     6061 attgcaggtc agatttaact tagacttgat gtccaacctt cagtgaggag cgcgctgact
     6121 gaacgcatga aacctgcaaa gctcttcccc atgttcctta agctttcggc ccgcccgtgc
     6181 ctcgttgtcg gtgcgggaac catcgctgaa tccaaaatcg ccagcctcct ggaggccggc
     6241 ggtcgcgtgc gcgtggtagc ccccgaagcc accccgcaag tgcgctcctg ggcgcaatcg
     6301 aacagcatcg aatggcttca gcgccctttc cgacccgacg atcttgaggg aatgtttttg
     6361 gttgtcgcgg ccacatcgtc cacgcaactt cacgagcgca tattcgaaga agcaacgcag
     6421 cgtgctgtgc tctgcaacat cgtggatgtc ccccctcttt gcgattttta ctatccctcc
     6481 gttgtgcagc gcggcgtcct gcagatcgcc atttccactg cgggacaaag cccggccctt
     6541 gcccagcgcc tcagaaagca gctcgaagat cagttcggcc cggaatacga agaatggctc
     6601 gcgcagttag gtgaaactcg cgaccagctc cactcctcta agctggatcc tgaggaacgg
     6661 aaacgcctgc ttcacgaaga cgccagcgag gaagccttcg aagcgttcct ccgaaatcgc
     6721 cggccatcga aacgctccgg ttgagtttcc cgcccgtttc accatctagc cgtttgccgt
     6781 cgcctgctat cagaaatgtc gatgcaaagc attcattctt ccgcagcttc acctgtcagc
     6841 gatagtgcta gtgttttagt gcggtattag cggctggcag tttgttcgaa tcgccgccgc
     6901 aagaagggct gctggctcaa gccagtaacg ggtaccatcc aatgcaggcg ccggccatga
     6961 agggcaaagt ctatctgatg cgagccggtc caggagatcc cgatctcctg accgtcaagg
     7021 cgatacgagt acttcgcgct gccgaggtcg ttctgcacga cgacttggtc tcgtccgaga
     7081 ttctcaactt gatcccagct tccacacagg tccgcaacgt tcacaaactc ggcttgcagg
     7141 tgggaaatct ccaggaaaaa atccattctt tgctcatcgc tgcagcgcgg caagggcatc
     7201 aggtcgtgcg gcttaaagcg aacgacccgc ttgccgtcga agagacggag gttctcgccc
     7261 aggccggggt cgatttcgaa gtgattcccg gcgctgcgtc ggccatggga gccgccgttg
     7321 gcgcaaactc tcgctgaccg gtttgcacga gtcttttcaa cactagattg cctcaatcgg
     7381 cggacccatt tctctgccgc tttcgacgaa acagtccctc ggattgctcg gccgcttcct
     7441 tcaccagaaa tcccatcttc ggatgtgacg gctcgaggtc ggcccgaatc ccccgtctct
     7501 gcagttcttc ggatgtcgtc ggcccgatcg aagccacaat cgcgcggtta acaccacgta
     7561 gcatcagctt ttccagcttc atctccgcgg cgatttggaa aaggtgcgca acctggacgc
     7621 cggtggtgaa gaggacaaca tctatctctc cttcggcgag tgctctaacg gccgcctgaa
     7681 ggggagcgag gtcttccggc agcgcccatt gataaatggg cacgcgtgta actgaagccc
     7741 cgcgttcacg aagcccgctc agcaattcag gattggatac accatactcc tgaacggcaa
     7801 cacgcgcccc gcgcaagcgc aactccgctc gggaggcgcc cgcctcatca agcgcgagca
     7861 gaagctcgcg ccacgtattc ggctcgggaa ccgtgatggc tggagtcacg ccaagttcac
     7921 gcagcaccgc cacaggcttc ggcccccggg gaaccacgct aacgcgttgg agagctgcca
     7981 cgtactcctc gcgtttgtag gtcgtctcaa ccacgctcag aagcgctcgt gccccgacgc
     8041 cggtcaagaa aatgaccatg tcgaattcgc cggcgaatag agccgccgca aaggccaatg
     8101 cttccctgtt ggattccagc ggcacttcgc gcatcgctgg cgcaacgatg ggttcgcccc
     8161 cgtagttgga aatcaatttg gcgagttctg gagcgcggcg gctctccagc gccagcactc
     8221 gcaatccggc aaatccgctc atgacagtga attgttaacc agaaatgccc ccgccgccag
     8281 cttcatctgc tggcgatcct cacaatgccg ggcttgggac aggggagtca cccgaagccg
     8341 ctcgattctg ttctccacaa gaaatcgcat tgccctgctt ctcggtacat gactccggca
     8401 cctcaataag aacgattccg gactcgatgc gcgtccgata cgtcgccacg caggctggct
     8461 ccgactctgg cttggtaaca cttcccgtct ccaggctaat tttccacgca tgcagcggac
     8521 aaaccacggc gttgcccgta acaattcctt ccgccaatgg gccgccctta tggggacatc
     8581 gtccatcgac ggccagcacg cgatcgccga ggttgaaaat cgcgatttca cgcccggcga
     8641 tgttcaccgc tcgcccttct cgcaaaggga tattttgcat cgccgtgatg cgaatccatc
     8701 gtttgttgct catgcctgca ccttttccag cggttcaatt tgcacgaact gcgtgggatg
     8761 ccgcggcgca tcgccttcca gccaggcgtc ccgcgaaatg cgtttggact ttcttagccg
     8821 ttctcgcaat ccctgctgga tcgccagcgg cgcgtagacc gtctcgcgcc gaaccttctc
     8881 gatactccag cgctcgacga aatcgtaggt gcgctccaga taattcgcgt tctcccggta
     8941 atactggaag aacaacgtgg ccgcctgcag cgcttcctcc gtcgactcga cggtgatcag
     9001 caggtcggcc ttgcgaactt tcttgccggc ggcgccgccc accaccacct gccagcttcc
     9061 ctcctgtccg accagcccga tgtccttcac cgtggcttcg gcgcaattgc gcgggcaacc
     9121 caccgcaccc atcttcagct tgtgcggcgt aaatagatgt tccagccgcc gctccagctc
     9181 gatgcccgct ttcgtcgaat cctgcacgcc gaagcggcaa aaatccgtgc ctacgcaggt
     9241 cttcaccatg cgcacgcctt tggtgtacgc ctggcccgaa ggcattccta aatccgccca
     9301 gatctggggc aagtctgcct tttttacgcc gagcaaatcg atgcgctggc tgccggtgat
     9361 cttcaccatc ggcacgttgt atttttcagc cacgtctgcg atgcgccgca attctttcgc
     9421 cgacgttacc ccgccgcgaa tgcgcggcac cacggaaaac gttccgtcct tctgaatatt
     9481 ggcgtgcacg cggtcgttga tgaagcgcgc cgagcgatcc tcatcgtgat cgccgcacca
     9541 cagcatgtcc accatatagc tcagcgccgg cttgcagatt tcgcagccta cgccattgcc
     9601 gtaaatctcc agaacttcct gcacggagcg caatctctgg ctccgcagga tctcgcgaag
     9661 tttgtcctcg ggaaacggca agcacttgca cagagatttc ttcgcttctt cctgaaactc
     9721 gggcgcaacc gctttcaaga ggtgctggca gacttccgtg cagctcccgc aacccgttga
     9781 cgctcgcgtg caatctttca actgcgcgag cgtgctgatg cccttctcat ggacggcctg
     9841 aatgatcgcc cctttggtaa cgccgttgca tccgcaaacg actttgctgt cggacatttc
     9901 cgcaacatcg aggccggtgt cttccacggg gcccggaaaa aaaagttggc gccgcatggc
     9961 cgtcaagtcc gtcgaagcgc gcagccaatc catgtagcga tggctgtccg aggtgtctcc
    10021 cactagaatc acaccggcca gtttgccgtc ccgtaccgtc agctttttgt aaatgcccag
    10081 cgcgggatct tcgtaacgca ctgcatccgt gcccggaact ttctcgttga aattgccggc
    10141 ggaaaataca tccacgccca t
//
LOCUS       DASYVO010000259         8181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_91447, whole genome shotgun sequence.
ACCESSION   DASYVO010000259 DASYVO010000000
VERSION     DASYVO010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8181
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91447"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VGR03_08620"
     CDS             complement(<1..201)
                     /locus_tag="VGR03_08620"
                     /inference="COORDINATES: protein motif:HMM:NF028194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994380.1"
                     /translation="MSNTNRQLRLQSRPKGLLAPGDLQLAESPIPELKDGEALARVKY
                     LSMDPTMRIWMVADTYLPAVPIG"
     gene            complement(203..706)
                     /locus_tag="VGR03_08625"
     CDS             complement(203..706)
                     /locus_tag="VGR03_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV1994381.1"
                     /translation="MSVNKVILVGRLGRDPETRYTGGGQAVANFSMATDESYKDKNGE
                     RQKRTEWHKIVVWGKQAEIAQQYLKKGSLIFLEGRIQSREWQDKEGQKRTSFEIVANN
                     FRMLGSRAEGAAAGGGGGGGAAAAARSGGGDEHEQPVPADDSYGGSGGGAPSGGGPEI
                     SDEDIPF"
     gene            884..2431
                     /locus_tag="VGR03_08630"
     CDS             884..2431
                     /locus_tag="VGR03_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994382.1"
                     /translation="MSRKKTVWLTALACAFVLVLAGTNRARAQQSGAPAAAPVGMFEG
                     HGDIGTVVHAGSVEYDAAKGSYTMAGSGENMWFTSDAFQFAWKKMSGDVTLTADISFL
                     GKGVHEHRKAALMIRESLDADSRYADIALHGNGMTALQYREAKGAITQEVQAGTWSPK
                     RLRIEKRGDFFTMWLADEKGEFHLAGGSARLALKEPFYVGIGVCSHDKDVVERAVFTN
                     VDLKTAAPAPAATTTLYSMLEIISVASTDRTAIYVAPERFEAPNWMPDGKTLLFNRNG
                     RLEKIPVTGGEPKVMDTGFATRCNNDHGISPDGTQVVISDQSQEEHRSLVYIVPVGGG
                     APRRITQKSPSYWHGWSPDGKTLAFVGERNGDFDIYAIPAAGGEEKQLTTAKGLDDGP
                     EYSPDGKYIYFNSERTGHMQIWRMRADGSEQEQVTFGEENDWFPHISPDGQQMVFLTF
                     DASVKGHPENKDVMLRLMTLTDKRITVLTKLFGGQGTMNVPSWSPDGKQFAFVSYQLV
                     PSGDTGR"
     gene            3320..3535
                     /locus_tag="VGR03_08635"
     CDS             3320..3535
                     /locus_tag="VGR03_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994383.1"
                     /translation="MAKVVLAGDPDAAKFLNAGHSYLEAKRWVNSHELLVVLEGHNDG
                     LPPGSFTLRYRVDLNGTVRKLSQHTEQ"
     gene            3904..4224
                     /locus_tag="VGR03_08640"
     CDS             3904..4224
                     /locus_tag="VGR03_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994384.1"
                     /translation="MSEPFRPYEKLVEIMVFGKKFQVPERNSLLRCFQFISPETIPYG
                     RFCWNQDCQYCRVTCQLPDDDEPHEMLSCKFIVMPGMEIVEMSQELKWCLRAKLPSDT
                     PVSS"
     gene            4470..5054
                     /locus_tag="VGR03_08645"
     CDS             4470..5054
                     /locus_tag="VGR03_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994385.1"
                     /translation="MTSTSALVCDGCGQAASAEHITRRLQRLEWTTRYRPVHINTLLL
                     GAFSPHDERDFLYAPGGEFRGEAALLLDALGISTAGKAAEVVYAEFQRAGFFLTHVLE
                     CPLGGDVGQGAEATARLTKRLPTVAIRIRRSLKPKRVMVVTEGLAQLADHILALHLGC
                     PVLLDHGKPFRLDSGAGENGAARLRAALAAAEAG"
     gene            5182..5619
                     /locus_tag="VGR03_08650"
     CDS             5182..5619
                     /locus_tag="VGR03_08650"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEV1994386.1"
                     /translation="MKKQAKFGVGISVIVLSLGFLAWLGYGESKTYYHTIAELETLKG
                     SAKSQRMRLGGTVAPGSIRHLAGRVDFVLEGEGKTLPVSYIGTDPLPDTFVDKSQALV
                     EGHPGPEGRFVAEVVQAKCASKYEAAPGGKGGAAPSTTERSGM"
     gene            5656..7713
                     /locus_tag="VGR03_08655"
     CDS             5656..7713
                     /locus_tag="VGR03_08655"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEV1994387.1"
                     /translation="MDVFGSFALVLAFICAVYAFCGGIAAIVTRHPLLIKSTRQAGMA
                     TCGLIFLATFSLEYLFFSDNFSIAYVVAHSNRDLSTFYKIAALWAGQEGSLLFWSFLL
                     AVYVLSVLITYRNKNGELMPYVGVVMVGVQIFFLTLNNFVASPFKVLASPGADGVMNY
                     VARADGNGLTPLLQYPEMVIHPPNLYSGYTGFTIPFAFALGALLARYPGEKWIHLTRK
                     WTMIAWMFQSMGILLGAHWAYAVLGWGGYWGWDPVENASLLPWLSGTAFLHSVMMQEK
                     RGMMKVWNVWLVFITFLLCILGTFLTRSGVVSSVHAFAQSSIGTWFVGFLALIIVVCF
                     GAYLKNRDYLKSENQLDSIISRESSFLFNNLILLVSCIAILSGTLFPVFSEWITGDRI
                     SVGAPFFNKVNIPIGLLLLFLTGVGPLLAWRKTSTESLKRNFGWPLGIGLAAGAIALV
                     FGFREIYSWVCLILCVFVASTVGLEFYRGAKVIRARSGASYVASAVDLTMRNTRRYGG
                     YIVHMGMVFVFIGLAGAAFNHDIQKDMRQGDTMQIGPYTLVLQGFDTKPERNYTAQRL
                     LVEVLRDKKPMMMLYPEKRRFATTAENGTMVAIYSTLKEDLYVVYAGNSPDTELPVIH
                     AYLNPLVKWIWFGGVVVVFGTIVALLPNRRAVLVLAGAQQPAAQPLAHGLTPSVTMRE
                     GHD"
     gene            7706..>8181
                     /locus_tag="VGR03_08660"
     CDS             7706..>8181
                     /locus_tag="VGR03_08660"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HEV1994388.1"
                     /translation="MTKARPIAAITMLAMFAIAPLLIAPVGAQQLSDRAKQVGGKFMC
                     MCSCSQVLTECNHVGCSVSASMLKELNLALSRGNSEDAITQMFVQEFGTKVYAEPPKS
                     GFSLVAWALPSIYLFVGTGVVIFVIFRWRKRPAQHAAPAGGAPGISAELLERARAQA"
BASE COUNT         1688 a         2121 c         2596 g         1776 t
ORIGIN      
        1 accgatcgga acggcgggta ggtaggtgtc cgcgaccatc cagatgcgca tggtgggatc
       61 catggagaga tacttcacgc gggcaagggc ttcgccgtct ttcaattctg gaattggcga
      121 ttcggctaac tgcagatcgc ccggcgcaag cagccctttc gggcgcgatt gcagacgaag
      181 ctggcgattg gtattcgaca cgttagaagg gaatgtcttc gtcggagatt tccgggccgc
      241 cgccggaggg agcgccacct ccactaccac cgtaagaatc gtcggcgggg acgggctgtt
      301 cgtgctcatc gccgccgcca gagcgcgctg ctgctgctgc tcctcctcct ccaccgccgc
      361 cagcggcggc accttcggcg cggctaccca gcatgcggaa gttgttggcg acgatttcaa
      421 agctggtgcg cttctggcct tctttatcct gccattcgcg ggactggatg cggccttcga
      481 ggaagattag cgaacctttc ttgagatact gctgggcgat ttcggcctgt ttgccccaca
      541 cgacgatctt gtgccactcg gtacgcttct ggcgttcgcc gtttttgtct ttgtaagact
      601 catccgtggc catggaaaaa tttgcgacgg cctggccacc gccggtatag cgcgtttcgg
      661 gatcgcggcc cagacggccg acgagaatga ctttatttac ggacatggaa atccccctgc
      721 gagggcgaag ataacatgcg aggacggaaa agggaaatcg aggcgcagac agagagcgaa
      781 tagcgccatg agatatagtt ccgcgccaaa aatgaaccga attgacaatc agccgagacc
      841 gtcctaacgt ttccattcgt tctggcgaat ggaggatttc accgtgagca gaaagaaaac
      901 cgtttggttg acggcgctgg cttgcgcttt cgtgttggta cttgccggga cgaaccgcgc
      961 tcgggcgcag cagtctggtg ccccggctgc cgcgcctgtc ggtatgtttg agggccacgg
     1021 cgacattgga acggttgttc atgctgggtc agtagaatat gacgccgcga agggaagcta
     1081 cacgatggcg ggcagcggcg agaatatgtg gtttaccagc gatgcattcc aatttgcgtg
     1141 gaagaagatg tccggcgacg tcacgctgac ggcggacatc tcgtttctcg gcaagggtgt
     1201 gcacgagcat cgcaaggcgg cgctaatgat ccgcgaaagc ctggatgcgg attcgcggta
     1261 cgcggacatc gcgctgcacg gcaacgggat gaccgcgctg caataccgcg aggcgaaagg
     1321 cgcgattacc caggaagttc aagctggcac ttggtcgccc aagcggctgc gcattgaaaa
     1381 acgcggagac ttcttcacca tgtggctcgc ggatgaaaag ggcgagtttc acctcgcggg
     1441 cggatccgcg cggctggcat tgaaggagcc gttttacgtg gggatcggcg tttgcagcca
     1501 tgataaggac gtggtggaac gcgcggtgtt cacgaatgtt gatttgaaga cggctgcgcc
     1561 tgcgccggca gcgacgacga cgctgtacag catgctggaa attatctcgg tagcatccac
     1621 ggaccggacc gcgatttacg ttgcgccaga gcggtttgag gcacccaact ggatgcccga
     1681 tggcaagact ttgctgttta accgcaacgg acgcctggaa aagattcctg tgacgggcgg
     1741 cgagcccaaa gtgatggata cggggttcgc gacgcgctgc aacaacgacc acggtatttc
     1801 gccggacgga acgcaggtgg tcatcagcga ccagtcgcag gaggagcatc ggtcgctcgt
     1861 ctacattgtg cccgtcggcg gcggcgcacc ccggcggatc acgcagaaat ctccttcgta
     1921 ctggcacggc tggtcgccgg acgggaaaac gctggcgttc gtcggcgaga gaaacggcga
     1981 tttcgatatc tatgcgatac ccgccgccgg aggcgaggag aagcaactca cgaccgccaa
     2041 aggattggac gatggcccgg aatactcgcc ggacggcaaa tatatttact tcaactccga
     2101 acgcaccggg cacatgcaga tctggcggat gcgggccgac ggaagcgagc aggagcaggt
     2161 aacgtttggc gaagagaacg attggtttcc gcacatttcg cccgatgggc agcagatggt
     2221 gttcctgacg tttgacgcga gcgtgaaagg ccatcccgaa aataaagatg tgatgctgcg
     2281 cctgatgacg ctgacggaca agaggatcac cgtattgacg aaactgtttg gagggcaggg
     2341 aacgatgaac gtgccctcat ggtcgccgga cgggaagcaa ttcgcgtttg tcagctatca
     2401 gttggtgccc tctggagaca ccgggaggta atcggaggca gcgaggcgtg cgagggttga
     2461 aggttaagag ttgaaagttg aggcggagag aagcctcacc cctgaagggg tgagctacag
     2521 cggcgtcgaa taggagtcta gttaaccata actggccccc ctccccctgt tttcgtaagt
     2581 gttgcatcta aaggacttag acttatcaca agtggtttag aatcaacact tgcaggctgc
     2641 cttataagtg ttgattctaa agatagttag agacggtcat ttagatcatt cttgcaattg
     2701 ttgattctaa ataagttagc agaaaacgcc agccagctta aaggttgggg agatgcggac
     2761 caaggtatcg gtgcacagaa ttacctgcaa agtagtttac cagagggata agaaaagtca
     2821 aggaaaattg aagtaagacg gcggttcact tgtccggttt cggcgttaat caggcatctc
     2881 ggcgctctcg gtgtctagag acgcaatcgc ttcaacggac atcggtcgtt cccgcgtgat
     2941 acatttcatt ctggcggagg cccagatggt ttcgcgagtg ttgacgatag gcgtgctcct
     3001 gattcagcta ccgtcgctgg ccgcgcaaac aaaatcagcc cacccgcgcg tcgtagacct
     3061 tcctcgtgca gcagttagtc gcattccatc acccaatgga aagtggaccc tgatttttga
     3121 gtgcccaaac gattgcagcg agagaaagct gtggattgag gaaaacgttt cgcatacacg
     3181 aaagctagtg aaagagtacg agagaagtct gggtatctct tgggctccag acagccgcat
     3241 gttcttcgtt aacgatgctt ctggtagcac cgagaccctg tgctacctat atgacccaac
     3301 cactttgaaa gagactgact tggccaaagt ggtcctagcc ggagaccccg acgcagcaaa
     3361 attcttgaac gctgggcact cgtatctcga agcgaagcgg tgggtcaatt cgcacgagct
     3421 acttgtggtc ttggaagggc acaacgacgg gttaccgcca ggcagtttca ctctgcggta
     3481 tcgcgttgac ctgaacggta ccgtccgcaa gctttcgcag cacacagaac agtagtcgca
     3541 ataaccatcg gaaaacgaac ccctgctccc agtgcccggg tgatcggaaa ctgattcgct
     3601 actgaaataa attgcaggcc ctgcgccgcc cttctctaga cttcgccgga cgggaggcag
     3661 ttcgtgacgg cggcgcgagg ctggaatttt gcccccgtag catattggga acgcgggcga
     3721 gacgcggcgt tgtgtgtgcg aaacgtgcgg gctactctgg ataggcaatc atctgtggcg
     3781 attggccgga tgcgggcgag accgagacgc gttgcgaagc aaaaagcaga tccctcgctg
     3841 cgctcgggat gacaagagac tcttagcgcg gcgcaggctg gtacacggag aacaggattg
     3901 gtcatgtcgg aacccttccg tccttatgag aagctcgtgg agatcatggt cttcgggaag
     3961 aaatttcagg tcccggagcg gaattcgctt ttgcggtgtt tccagttcat cagccccgaa
     4021 acgattccgt acgggcgctt ctgctggaat caggattgcc agtattgccg cgtgacgtgc
     4081 caactgccgg atgacgacga accacacgag atgctgagct gcaagttcat cgtgatgccc
     4141 gggatggaga ttgtggagat gagccaggaa ttgaagtggt gcctgcgcgc taagctgcct
     4201 tcggacacgc cggtttcttc ctgagttttc ttttgtcagt tttcagtaga cagtaagaga
     4261 aaaaatgggg gctgtgcctg agcctacgct cttgttttca cgttttagtt ttcagtggga
     4321 gcagaatcga gagattcggt ctaaggcgcg gcttttgatt cccatttgcg ctgacgaaag
     4381 aaaagccggg ccgaagcccg gccattagaa agaccgtcac atcgagattt gcagtcgcag
     4441 cgacattcca ctacattcgg aattggatta tgaccagcac aagcgcattg gtgtgtgacg
     4501 ggtgcgggca ggccgcgtcg gcggagcata taacgcggcg gctgcagcgc ctggaatgga
     4561 cgacgcggta ccggccggtc catatcaata cgcttctgct gggtgccttc tcaccgcacg
     4621 acgagaggga ttttctgtat gcgccaggcg gagagtttcg cggggaagcg gcgctgttgc
     4681 tggatgcgct gggaatctcc accgcaggaa aagcggcgga ggttgtgtac gcggagtttc
     4741 aacgggcggg attttttctg actcacgtcc tggagtgccc gctcggcggg gatgtcggac
     4801 agggggccga ggctacggcg cggctgacaa agcgcctgcc aacagtggcg atacggatcc
     4861 ggcggtcgct caaaccgaag cgtgtgatgg tcgtcactga gggactggcg cagctggcag
     4921 accatattct agctctgcat ttgggctgcc cggtgctgct ggatcatgga aaaccgttcc
     4981 gactggacag cggcgccgga gagaatggtg ctgcgcgttt gcgagcggcg ctggcggctg
     5041 cggaagcggg gtgaacgaag ggacaggctg cggctggcca ttggtagcaa gcgcgatggt
     5101 accgaaagcc ggtagaccac ttttttaggt gtgatagaat ggggcgaatt ggcgggctga
     5161 gagcggcgta ggagtaccat cgtgaagaaa caagcaaaat ttggagtggg aatcagtgta
     5221 atcgtgcttt cgctcggttt tctggcgtgg cttggttatg gagagagcaa gacttactac
     5281 cacacgatcg ccgagcttga gaccttgaag ggctccgcaa aaagccaacg tatgcgcctc
     5341 ggcggcacgg tcgcgccggg aagtattcgc cacctcgccg gccgcgttga ttttgtgctg
     5401 gagggggaag gcaagacgct tccggtgagt tacattggca ccgacccgtt gccggataca
     5461 tttgtagaca aatcccaagc cctcgtggag ggccaccccg gtccggaagg acgatttgtc
     5521 gccgaggttg tgcaggcgaa atgcgcttcc aagtatgaag cagcgcctgg aggcaagggt
     5581 ggcgccgcgc ccagcaccac cgagcggagc gggatgtagg gcgcccggga tctccggggg
     5641 ataggcgggg ttgccgtgga tgtattcggt tcttttgcac tcgtcctagc gtttatatgc
     5701 gcggtctatg cgttctgcgg aggcattgcg gcgatcgtca cgcgccatcc gctgctgatc
     5761 aaaagtacgc gccaggccgg gatggccacc tgcggcctga ttttcctcgc cacgttcagc
     5821 ctcgaatatc ttttcttcag tgataacttt tccatcgcct acgttgttgc gcacagcaat
     5881 cgcgacctgt cgacatttta taagatagcc gcgctatggg cggggcagga aggttcgctg
     5941 ctgttctgga gcttcctgct ggccgtttat gtgctttcgg tactgatcac ctaccggaac
     6001 aagaatggcg agttgatgcc gtacgtcggc gtagtgatgg tcggcgtgca gattttcttc
     6061 ctgacgctga ataacttcgt tgccagcccc ttcaaagtgc tcgcgtcgcc gggagcagat
     6121 ggcgtgatga attacgtcgc gcgtgccgat ggcaacggat tgaccccgct gctgcaatac
     6181 ccggaaatgg tgatccatcc gccgaacctg tattcggggt acaccgggtt cacgattccg
     6241 tttgcctttg cgttgggcgc attgctggca cgttacccgg gcgagaagtg gattcatctg
     6301 acgcgcaagt ggacgatgat tgcctggatg tttcagtcca tgggcattct gctcggcgcg
     6361 cactgggcct acgcggtgct cggctggggc ggttactggg gctgggaccc ggtcgagaac
     6421 gcatcgctgc tgccgtggct gtcgggcacg gcgtttctgc actccgtgat gatgcaggaa
     6481 aagcgcggca tgatgaaggt ctggaatgtg tggctggtgt tcatcacgtt cttgctttgc
     6541 atcctcggga cattcctgac ccgcagcggt gtggtcagct cggttcatgc cttcgcgcaa
     6601 tccagcatcg gcacctggtt cgtggggttc ctcgcgctga tcatcgtggt gtgcttcggg
     6661 gcttatttaa agaatcgcga ctacctgaaa agcgagaacc aactcgattc gatcatttcg
     6721 cgcgaatcga gcttcctgtt caacaacttg attctgttgg tgtcctgcat cgcgattcta
     6781 tcgggcacgc tctttccggt tttttccgag tggatcaccg gcgaccgcat cagcgtgggc
     6841 gcaccgttct tcaataaggt gaatattccc atcgggctgc tgctgctgtt tttgacggga
     6901 gtcggtccgc tgctggcgtg gcgcaagact tcgacggaaa gcctgaagcg gaatttcggc
     6961 tggccgctgg gaatcgggct ggccgcaggt gcgattgccc tcgtgtttgg cttccgtgag
     7021 atctattcgt gggtttgcct gattctgtgc gtatttgtcg cgtccacggt gggcttggaa
     7081 ttctatcgcg gcgcgaaagt gattcgcgcg cgatcgggcg cttcgtacgt ggcatcggcg
     7141 gtggacctca caatgcgcaa tacgcggcgt tacggcggct acatcgtgca tatggggatg
     7201 gtatttgtct ttatcgggct ggccggcgcg gcattcaacc acgatattca gaaagatatg
     7261 cggcagggcg ataccatgca gatcgggccg tacactttgg ttctgcaggg cttcgatacc
     7321 aagccggaaa ggaattacac cgcgcaacgc ctgctcgtcg aagtgctgcg ggacaagaag
     7381 ccgatgatga tgctgtatcc cgagaaacgg cgctttgcga ccaccgcgga aaacggcacg
     7441 atggtggcga tttattcgac cctgaaggaa gatttgtacg tggtctacgc cggcaacagt
     7501 cctgatacgg aactgcctgt gattcacgcc tatctgaatc cgctggtgaa gtggatctgg
     7561 ttcggcggag tggtggtggt atttggaacg atcgtggctt tgttgccgaa tcgccgtgcg
     7621 gtattagtgc tcgcgggcgc ccaacagcca gcggcacaac cattggcaca tgggctcacc
     7681 ccgtcggtta ccatgcgaga gggccatgac taaggcgcgc ccgattgccg caatcacgat
     7741 gctggcgatg ttcgcgattg cgccgctgct gatcgcaccc gttggggcgc agcaactttc
     7801 cgatcgcgca aagcaggtcg gcggcaaatt catgtgtatg tgcagctgca gccaggtgct
     7861 gacggagtgc aatcacgtgg gctgcagcgt gtccgcgagc atgctgaagg aattgaacct
     7921 ggcattgtcg cgcggcaatt ccgaggatgc catcacgcaa atgttcgttc aggaattcgg
     7981 aacgaaagtg tatgcggaac cgccgaaatc gggtttcagt ctggttgcct gggcattgcc
     8041 gagcatttat ctgttcgtgg gcactggagt ggtgattttc gtgatcttcc gctggcggaa
     8101 gcgccccgcg caacacgcgg ctcccgcggg cggcgccccg ggtatttccg ccgagttgtt
     8161 ggagcgtgcc agagcgcagg c
//
LOCUS       DASYVO010000260         5340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_76102, whole genome shotgun sequence.
ACCESSION   DASYVO010000260 DASYVO010000000
VERSION     DASYVO010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76102"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3602)
                     /locus_tag="VGR03_08665"
     CDS             complement(<1..3602)
                     /locus_tag="VGR03_08665"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HEV1994389.1"
                     /translation="MKLQSLMLRNSLFLLTLILTLPAFRAAAQSKPDSAPAAQAAAIP
                     ARITQAIDETQLVRLQGNVHPLARLEFDRGIVSDATPMKRMMLLLQRSPEQQAALRQF
                     MDEQLSKDSPNFHKWLTPEQFGKQFGPADADIQTVTDWLTRQGFQGIKVAAGRTAIEF
                     SGNVGQVRNAFHAGIHQFNVNGESRQANVSDPQIPAALTPVVAGVLTLHNFPRKSMRH
                     IAGTFTRTADGRVEPQFTGSTGQFFVVGPADFAKIYNIPAALDGTLQHIAIVADSNIN
                     PQDVTDFRTLFSLPTNPPNIILNGPDPGLNVDEGEADLDVQVAGMVAPKATIDLVVSE
                     DTLTAAGIDLSSFYIIDNNIAPVMSLSFGACEAGLTTAGNNFFNTLWEQAAAQGITVM
                     VASGDPGSAGCDNFNTAQVATHGLAISGIASTPFNVAVGGTDFDDFSPSPIQSTFWAA
                     INGAGKESALGYIHEIPWNDSCAATATSSTRNTVCAIPNTNPLLNIVGGSGGPSSVYA
                     KPAWQSGITPSGIAAGDNHRYIPDVSLFASDGSQSKSFYLVCEADAITPGGSPSCASS
                     GPFSFLGVGGTSASSPAFAGIMALINQNNGRQGNSNPVLYKIATTAGQSCNSSTEALS
                     GSTCAFNDVTKGNNSVPCAASSPNCSSTTAGTNGVLVSPASAITPAWTTAPGSGALPG
                     YDLATGLGSVNVANLATQWPLAVGKFTGTTTTLTLNSGTAVNITHGTPVTAKVTVAPS
                     SGTATPTGDVSLLAPTAVNGGIGDVALSGGIANITGVILPGGSYNVNARYGGDTTFAS
                     STSTTGVPVVVGKEPSRLQYGIVTFASNGAITSTNATSFAYGSPYILRMDILNSTANL
                     CTPLAATPVLTGCAFDATGTVTITDNGSPLDTGTFKINSAGSAEDQPIQLTGGTHALS
                     AIYSGDISYSPVTAPVTDTVAVSKATTATALTTSAPTVTTGTNVTLTATISSGSNSSV
                     GATGTVTFFNGGTQIGSPVTVAQTAASATAGAGGTAALTTSFNTTGTKSITATYNGDT
                     NYALSSATAVTVTVSGTGSFTVSGTAATATAGSTGTSTITVTPTGGFTGIVNVTCPAA
                     GLPAGVTCTPNPLAINVMSAIAATSQLTVNVAAPSTALSASAAPAEPTLYAAGTIPPS
                     GGKGWWTLSAGTGLAAMFLLFLPGRKRYRAALGLGLVCVLCFTLGCSKSYGGGGG"
     gene            complement(4265..4885)
                     /locus_tag="VGR03_08670"
     CDS             complement(4265..4885)
                     /locus_tag="VGR03_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994390.1"
                     /translation="MAEEHSTILDWMNLPHGAAEVSLWAGLHDAQIVSIQSNLLERTI
                     TLHLESDHLLEFHKLPLDMQFLLRLEGVQSARVVHYAVWPGKFSVPAGAAREEEARLI
                     ATYQSKWHEESLSWTELESAVTTECKQVLDIADATLATSADNSVALRISGLLNYTSYR
                     ELFLRAERLTLTRGDGQDLGIAGLLKMGEAYWDAADRQEKEDGPST"
     gene            5039..5290
                     /locus_tag="VGR03_08675"
     CDS             5039..5290
                     /locus_tag="VGR03_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994391.1"
                     /translation="MAEPTYEELKAKIAEMEKQGGGRRTGSLEFRVGEKGGVSVYGLG
                     RFPVTLYYEQWIRLLEAVPQLRAFLDDNKAEGKLKLKEK"
BASE COUNT         1098 a         1504 c         1639 g         1099 t
ORIGIN      
        1 cccccgccgc cgccgtaact cttgctgcac cctagcgtga agcagagcac gcaaaccagg
       61 ccaagcccca gcgccgcgcg atagcgcttc cgcccgggta ggaagagaag gaacatcgct
      121 gcgaggccgg tgccagcgct cagcgtccac cagcccttgc ctccgctcgg aggtatcgtc
      181 ccggctgcat agagcgtggg ctctgccggc gctgccgatg ccgacaacgc tgtggatggt
      241 gccgctacgt tcacggtcaa ttgactcgtt gccgcgattg ccgacatgac gttgatcgcc
      301 aggggatttg gtgtgcaagt tacacccgca ggcaatccag cggcaggaca agtcacgttt
      361 acgataccgg tgaaaccacc ggtcggcgtc accgtgatgg tggatgtgcc agtggatccc
      421 gccgtcgccg tcgccgcggt gccgctcacg gtaaacgaac cagtgcccga cacggtgacg
      481 gtgaccgccg tggcggacga tagcgcataa ttcgtatcgc cgttataggt cgcggtaatt
      541 gacttcgttc ccgtagtgtt gaacgaggtt gtcaacgccg cggtgcctcc tgctccggcg
      601 gtcgccgatg ctgccgtttg agccacagtg acggggcttc caatttgcgt tcctccatta
      661 aagaacgtca ccgtcccggt tgcgccaacc gagctgttgc ttcccgagga aatcgttgcc
      721 gtcaacgtga cattcgtgcc ggtcgttact gtaggtgcgc tggtggttag ggccgtggct
      781 gtcgtcgcct tcgaaacggc aacggtatcc gtcacggggg cggtgacagg gctgtagctg
      841 atgtcgcccg aatagatcgc cgatagcgcg tgggttccac ccgtaagttg aatcggctgg
      901 tcctcggcgc ttcccgcact gttgatctta aatgttccag tgtcgagagg gcttccgttg
      961 tctgtgatgg ttaccgtccc cgttgcgtcg aaagcacaac cagtgagaac gggagtcgct
     1021 gcaagcggcg tgcataggtt tgcggtgcta ttcaagatgt ccattcgcaa aatgtagggg
     1081 gagccgtacg cgaacgatgt cgcattcgtg ctggtgatag caccgttaga agcgaatgta
     1141 acgattccat actgcagtcg gctgggttcc ttgccaacta cgacaggcac accggtggtt
     1201 gaggtgcttg aagcaaaggt ggtgtcgccg ccatagcggg cgttcacgtt gtagctgccg
     1261 cctggaagaa tgacacccgt aatgtttgcg atgccgccgg acaaagcaac gtcaccgatg
     1321 cccccgttga ctgcggttgg tgcgagaagc gagacgtccc cggtgggtgt ggccgttccc
     1381 gaagaaggtg caaccgtcac tttagctgtt accggggtac cgtgcgtaat attgaccgcg
     1441 gtcccgctgt tcagagtaag cgtggttgtg gtgccagtga atttgccgac ggccaggggc
     1501 cattgcgtgg cgaggttggc gacgttcacc gagccgaggc cggtcgccag atcgtatccg
     1561 ggaagcgcac cgctgccggg tgctgtcgtc caagcgggag ttatcgcgga ggctggactt
     1621 acaagaacgc cattggttcc agcggtcgtg ctgctgcaat tcggagaact tgccgcgcaa
     1681 gggaccgagt tgttcccctt ggtgacatcg ttgaatgcac acgtggagcc ggaaagggcc
     1741 tcggtcgaag agttgcagct ttggccagcg gtagtggcaa tcttatataa cacgggattg
     1801 gagttgcctt ggcggccgtt attctgattg atcaaggcca tgatgcctgc aaatgctggc
     1861 gaggatgcag aagtcccgcc cacacccaag aaactgaaag gaccgctgga cgcgcaagac
     1921 ggcgacccgc caggagtaat tgcatccgcc tcgcacacca ggtagaagct cttactttgc
     1981 gatccgtcgc tggcgaaaag agaaacgtcg gggatgtaac gatggttgtc gcctgccgca
     2041 attccgctcg gcgtgatgcc gctctgccag gcgggtttcg cataaacgga gctaggtccg
     2101 ccactaccac caacgatgtt cagcagagga tttgtgttcg ggatggcgca gactgtgttg
     2161 cgagtggagc tggttgccgt tgccgcgcaa gaatcgttcc agggtatctc gtgaatgtaa
     2221 cctagggcgg attctttacc cgcgccattt attgctgccc agaaagtgct ttggatgggt
     2281 gaaggggaga agtcgtcgaa atccgttccg cccactgcaa cgttgaacgg cgtggaagcg
     2341 atcccactta tcgcgaggcc gtgcgtggcg acctgcgcgg tattgaaatt gtcgcacccg
     2401 gccgagccag gatctccgga ggccaccatc accgtgatac cttgcgccgc cgcctgttcc
     2461 caaagcgtgt tgaaaaagtt gttgccagcg gtagtcaaac cagcttcaca ggcgccgaaa
     2521 ctcaggctca tcaccggagc gatattgtta tcaatgatgt agaacgaaga gaggtcgatt
     2581 cccgccgcgg tcagggtgtc ttccgacacc accaagtcga tcgtggcttt cggggcgacc
     2641 atgcccgcca cttgcacgtc caggtcggct tcgccttcgt ctacattcaa accggggtcg
     2701 ggcccattaa ggatgatatt gggaggattg gtcggaaggc tgaacaaggt tcggaaatcg
     2761 gtcacgtctt gtgggttgat gtttgaatca gccacgatcg cgatatgctg cagtgttccg
     2821 tcgagcgcgg ccgggatgtt gtatattttc gcgaaatccg ccgggccaac gacgaagaac
     2881 tgcccggtgc tcccggtgaa ttgaggttca acgcggccat cggcagtgcg cgtgaaggtg
     2941 ccggcgatgt ggcgcatcga ctttcgtggg aagttgtgga gcgtcagcac cccggcgacc
     3001 acgggcgtta gcgccgcggg gatttgggga tcgcttacgt tagcttggcg ggattcgccg
     3061 ttcacattga attgatggat tcccgcatga aacgcatttc gcacctgccc cacattcccg
     3121 gaaaactcga tggccgtgcg tccggctgcc accttgattc cctggaatcc ctgacgtgta
     3181 agccagtccg tcactgtctg aatgtccgcg tccgcgggcc caaactgctt gccaaactgt
     3241 tccggggtga gccatttatg gaaattgggc gagtccttgc tgagttgttc gtccatgaac
     3301 tgccgcagag cggcctgttg ctcggggctt cgctgcaaga gcaacatcat tctcttcatg
     3361 ggtgtggcat cggagacgat tccccgatcg aattcgagcc gcgcgagcgg gtgcacgttg
     3421 ccctgcaggc gcacgagttg cgtttcatcg atggcttggg tgatacgcgc ggggatggct
     3481 gcggcctgag cggctggcgc gctatcgggc ttggattgcg cggcggcacg aaacgccggg
     3541 agcgtcagaa tcagagtaag aaggaaaagg gaattgcgaa gcatcagaga ttggagtttc
     3601 atattccgtc caggctgcaa atttgaattc gattccaatg caactcaact gaactcaagg
     3661 caaccggcct cccgaccggg tccccgagaa gctcggggcg ggcgggacaa gttccggcgc
     3721 ggccatcggc accgagggac agcttccaga aatggcttct cgcggactgg ggcttggacc
     3781 ggaaacgact cactgtagaa cgcgtaaagg gtatcacagg caattaaaag acactctagt
     3841 accttggcgg cggagcgcgc tcggcgaagc gctagcaaac tggagcctca cccgcacact
     3901 gcaaccatgg ccattctgat gcacaccgga ttccgcggga tgcttgagct gtttgttggc
     3961 gggcaccgtg cctcagggtg atagttcgcg gcgctcgtgt tggcagaaac tatccctgtt
     4021 actcctattc gaccactcca gtgccacttt tattcccacc gaagagagct cgttccctct
     4081 atctcgccta ggcaccggat agggcgcagc ggtgctgcgc ccctgcgaag acaaaagcag
     4141 ggcggcggag agagaagcag ggcgaactga agttcgcccc tacatggaca agagctacaa
     4201 agaaagttca ctccactgac cccgcatggt gagaaatcgg gactggcgct ggccccttcc
     4261 gatttcaggt cgaggggcca tcctcttttt cctggcgatc ggccgcatcc cagtacgcct
     4321 ctcccatctt caggagtccg gcaattccca agtcctggcc atcgcctctt gtaagcgtga
     4381 ggcgctccgc tcgcagaaat agttcacggt aggaggtgta gttcagaagc ccgctgatgc
     4441 gcagcgccac ggaattgtcc gcggacgtag ccagcgttgc gtccgcaata tcaagcacct
     4501 gtttgcattc cgtggtgacc gcgctctcca attcggtcca actcaaagat tcctcgtgcc
     4561 acttggattg gtacgtggca atcaaccgcg cttcctcttc gcgagcggcg ccagcgggaa
     4621 ccgaaaactt tcccggccac acggcatagt gaactacccg cgccgattgc actccctcga
     4681 ggcgcagaag gaactgcatg tccagcggca gtttgtggaa ttcaagcaag tgatcgcttt
     4741 ccagatgcag cgtaatggtg cgctccagga gattggattg gatcgagacg atttgcgcat
     4801 cgtggaggcc ggcccacaga gacacctccg ccgcgccgtg cggcaggttc atccaatcga
     4861 ggattgtgga atgctcttca gccatgcgcc accgtttcaa gcgaattcgc gggccgagta
     4921 tagcccgcac cccgtcaaag cgaaacgcat tgtataatcc ggctgctact tcatgcacgc
     4981 agagaagaaa gagagtgcgc actgaagtgc gcccctacat ttcaacagag gagataccat
     5041 ggccgagcca acttacgaag aactcaaagc aaaaatcgcg gaaatggaaa aacagggagg
     5101 cggacggcgc accggctcgc tcgaattccg cgtcggcgaa aaagggggcg tcagcgtata
     5161 cggcctcggg cgctttccgg tgacgctcta ctacgaacaa tggatccggt tgctggaggc
     5221 agtaccgcag cttcgcgcat tcctcgatga caacaaagcc gagggcaagc tgaagctcaa
     5281 ggaaaaataa tcatccatga ttccgtttga ccactgagga cggagtcaga tcttggactg
//
LOCUS       DASYVO010000261         2875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_107881, whole genome shotgun sequence.
ACCESSION   DASYVO010000261 DASYVO010000000
VERSION     DASYVO010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107881"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VGR03_08680"
     CDS             complement(<1..483)
                     /locus_tag="VGR03_08680"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV1994392.1"
                     /translation="MESKREQALVGLFVLVAGAVLVATVFALTGAFGGTAATYRAYFP
                     FAGGLEPGATVRYAGGPKVGRVEKLQLDPKDASRIEITFSVKSGLPVKTDSHVRIMSL
                     SPLGDNHLEIVPGSEKAALAATGTILPSDPYVDFNALTAKINDIAPQAQQLLKTLNDR
                     A"
     gene            complement(507..1340)
                     /locus_tag="VGR03_08685"
     CDS             complement(507..1340)
                     /locus_tag="VGR03_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1994393.1"
                     /translation="MEQTNSAATQPEAMISLRNLRVSYGEREILHGIGFDVMRGETLV
                     ILGGSGSGKSTLLRTLVGLERPSSGEIWLKGKNIAAISSHEMDEIRKKIGMSFQGGAL
                     FGSMTVGENVALPLREHTKLEDSTIEIILRLKLDQVGLSGFENYMPSQLSGGMKKRAA
                     VARALAMDPEILFFDEPSAGLDPIIAAGVDELILDLKKAFHMTIIVVTHELASAFLIA
                     DRMVLIDKGNVVAIGSKEEMRSSTHPRVRQFLDRIAEPEVSQELDYLQMLTEERRRPG
                     R"
     gene            complement(1357..2154)
                     /locus_tag="VGR03_08690"
     CDS             complement(1357..2154)
                     /locus_tag="VGR03_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1994394.1"
                     /translation="MELTTQIGESVTDGLAYLGALTSLGGRAAYFTFIAPFQGKPLRL
                     QRAVSQAMQTGVGALPILSLITFFIGLILALQSAYELRKFGALNYVASAVAISMTREL
                     GPLITAIVVIGRSGSAFAAEIGTMKVTEEIDALETMAIDPIHFLVTPKFIAMIVMLPC
                     LTIWANSMGILGGALFGVAQADFTVVRYIKSSLDALFLRDITTGLIKSVMFGITITAV
                     GCHEGLSTGAGAEQVGRSTTRAVVVSIFLVILVDLVFTALFFFMSVR"
     gene            complement(2197..2529)
                     /locus_tag="VGR03_08695"
     CDS             complement(2197..2529)
                     /locus_tag="VGR03_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV1994395.1"
                     /translation="MQIAARHLDKITIFDISGDIDLATSPELRKALLRELRELKMPRV
                     VLNLQAVRYIDSSGVASLVEGLKASRDVGSRFILFGLNKTIREVLQLSKLVRIFEIYE
                     NEEQSVAP"
     gene            complement(2542..>2875)
                     /locus_tag="VGR03_08700"
     CDS             complement(2542..>2875)
                     /locus_tag="VGR03_08700"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1994396.1"
                     /translation="VLAVDEGLTNIIRHAYGGNAERPIEASFRRIHARCDGKSGDALE
                     IVLEDRGAAVNPKKLCGRTLEDIRPGGLGLHFIRESMDTVEFSRRKGRNQLRLVKILH
                     VQGPRKAS"
BASE COUNT          617 a          915 c          755 g          588 t
ORIGIN      
        1 cgcacggtcg ttgagcgtct taagaagttg ctgcgcctgg ggcgcaatgt cgttgatctt
       61 tgccgtgagc gcattgaagt ccacgtaggg gtcggacggc aggatcgttc ccgtcgcggc
      121 gagcgcggcc ttttcgcttc ccggaacaat ttccaaatga ttgtccccca gcgggctcaa
      181 actcatgatt cttacatggc tatccgtctt gacgggcaag ccggacttca cgctgaacgt
      241 tatctcgatg cgcgaagcat ccttcgggtc cagctgcaat ttctcgacgc ggccgacttt
      301 cggcccgccc gcgtagcgca ccgtcgcgcc gggttccaat ccgcctgcaa acggaaagta
      361 agcgcggtac gtcgcggccg tgcccccaaa cgcgccagtg agggcaaaaa ccgtggccac
      421 cagcacggcg ccggcgacca ggacaaacaa ccccaccagg gcttgctcgc gtttcgattc
      481 catcacgcac ctctcttccc cgctcttcaa cggcccgggc ggcggcgttc ttccgtcagc
      541 atttgcaaat agtccaattc ctgcgaaact tccggctcgg caatgcggtc gaggaattgc
      601 cgcacgcgcg gatgcgtgct tgaacgcatt tcctctttgg agccgatggc gaccacgttg
      661 cccttgtcaa ttaaaaccat gcggtcggca atcagaaaag cgctggccag ttcgtgagtg
      721 accacgatga tggtcatgtg gaaagccttc ttaagatcga gaataagctc atccacgccg
      781 gcagcaataa taggatccag tcccgcggaa ggctcatcga aaaaaagaat ctcaggatcc
      841 atggccagcg cgcgagccac cgctgcgcgc ttcttcatgc caccgctcaa ctgcgacggc
      901 atgtagtttt cgaatccgga cagtcccacc tgatccagtt tcagccgcag gatgatttcg
      961 atggtcgaat cttccagctt ggtgtgctcg cgcagcggca aggccacgtt ttctccgacc
     1021 gtcatcgagc caaacagcgc gccgccctga aacgacattc caattttctt ccggatttcg
     1081 tccatttcgt gggatgaaat cgccgcgata tttttccctt tgagccagat ctcgcccgaa
     1141 ctcggccttt ccagccccac cagggtgcgc agcagcgtgc tctttccgga accagacccg
     1201 cccagaatca ccagggtttc gccgcgcatc acgtcaaaac caatgccgtg gagaatttca
     1261 cgttcgccgt aactgacgcg aagattccgc agcgagatca tcgcctccgg ctgcgtcgcc
     1321 gcgctgttgg tctgctccat ggcgatattc caaaccctac cttacactca tgaagaaaaa
     1381 gagcgccgta aagacgaggt ccaccagaat caccaaaaat atggaaacca ccacggcgcg
     1441 agtggttgat cgcccgacct gctccgcgcc tgcacccgtg gaaagccctt cgtggcatcc
     1501 cacagccgtg atcgttatac cgaacataac gctcttgatt aatcccgtcg tgatgtcgcg
     1561 cagaaagagc gcgtccaaag acgacttgat atatcggacc actgtgaaat ccgcctgcgc
     1621 cacgccaaag agcgctccgc ccagaatccc catggagttc gcccatatcg tcaggcatgg
     1681 cagcatcacg atcatggcaa taaattttgg cgtaaccagg aaatgaatcg gatcgatcgc
     1741 catcgtctcc agcgcgtcga tttcttccgt gaccttcatc gtgccgattt ccgcggcgaa
     1801 cgccgaaccg gaccgtccga taaccacgat cgcggtaatc agcggcccga gttcccgcgt
     1861 catggaaatc gccacggcgc tggcaacgta gttcaaggcg ccgaacttgc gcagctcgta
     1921 agcgctttgc agcgccagaa tcagcccgat gaaaaacgta atcagggaca ggatggggag
     1981 cgccccaacg ccggtctgca tggcctgcga aacggcgcgc tgcaagcgca gcggcttgcc
     2041 ctggaaagga gcgatgaatg tgaaataggc cgcacggccg cccagcgagg tcagcgctcc
     2101 cagatacgca agcccatcgg taaccgattc cccaatttgc gtcgtcagtt ccacagtgct
     2161 ctcgtcgggc ttcctcggat tctgggttta gcgggctcag ggcgccacgg actgctcttc
     2221 gttctcgtaa atctcaaaaa ttctcaccaa tttggagagc tgcagaactt cccggatcgt
     2281 tttattcagc ccgaacagga tgaaacgaga gccgacatcg cgcgacgctt tgagcccttc
     2341 caccagggag gccacgccgg agctgtcgat gtaccggact gcctgcagat tgagcaccac
     2401 ccgcggcatc tttagttcgc gaagttcgcg caaaagcgct ttccgcagtt ccggtgaagt
     2461 cgcgagatcg atgtctcctg aaatgtcaaa aatggtgatc ttgtccaaat ggcgcgcggc
     2521 aatctgcaag ttggattctc ctcaggaagc tttccggggc ccctgcacgt gcaaaatctt
     2581 caccagccgc agctgattcc tgccctttct gcggctgaat tctaccgtat ccatgctctc
     2641 acgtataaag tgcaggccca acccccccgg ccggatatct tcgagcgtcc gcccgcacaa
     2701 cttcttcgga ttcaccgccg caccgcgatc ttccagcaca atttcaagag catctccgga
     2761 cttgccatca cagcgcgcgt gaatcctgcg gaacgacgcc tcgatcggcc gctctgcgtt
     2821 tccgccgtag gcatggcgaa tgatatttgt gagcccttcg tccacggcga gcacc
//
LOCUS       DASYVO010000262         1987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_21119, whole genome shotgun sequence.
ACCESSION   DASYVO010000262 DASYVO010000000
VERSION     DASYVO010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21119"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="VGR03_08705"
     CDS             complement(<1..1315)
                     /locus_tag="VGR03_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994397.1"
                     /translation="MEALSLRILLPIAALLYVAGVNPARAQAPLEPAQMSQRTLFYVI
                     WHGVPGPEARKANSLLALWDDADFAPMRSAIAAGMLNSSGEKSDRTKLTPEQVQECAA
                     LLENSFTIGYLSEPAKRSVSNGAASADAKPPAWNGMFFAYDRTGKEALLSKAILRLRA
                     EEKEAPRLSQVTIGGVEVLKVEGKSGATYWAEHGKFAVSAGERSVMEEILGRLDGKVS
                     GAASLAQSAAYQEAQANLGSGLLEFFLRIPDLEKFAADSKAGTFQVRPLLDAAKLDAV
                     HSLSGHITFEGAKTHVQAAILGDAVPGTPFDIWSAGQPSPASLTFVPADAVSYTTGQV
                     NFQGIYDMVKRVARAAFPQGQQGNADLIDTIAQQKLGMPVPDALGLLTGEIASMQTDP
                     SMDSTKQVYFFGIRKKPETLKLMRTVFSDQLTSERNEGDVTFLKVS"
     gene            complement(1495..>1987)
                     /locus_tag="VGR03_08710"
     CDS             complement(1495..>1987)
                     /locus_tag="VGR03_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994398.1"
                     /translation="EVGGAKKDIEATKNDLEATKGKLERSLGDMNVMSGLIAHSREDL
                     EDLKRRGDRNYYEFTLQRSKSAQRVGPVQMSLNKTDPKKAKYTVTVVADDKTIEKKDK
                     TSGEPVQFYVKGSSRMAPYEIVVFDVGRNQITGYLATPKEGGAAAPPAAATPAPTATP
                     TKP"
BASE COUNT          384 a          556 c          595 g          452 t
ORIGIN      
        1 gagagacctt caggaaagta acatcgcctt cattgcgctc agacgtgagt tgatcgctga
       61 aaacggtacg catgagtttg agggtctccg gtttctttcg aatgccaaaa aaatagacct
      121 gtttcgtgga gtccatcgac gggtcggtct gcatcgacgc gatttctccg gtgagcaacc
      181 caagcgcatc cggaacgggc attcccagtt tctgttgtgc gatcgtgtca atcaagtcgg
      241 cgttgccctg ctgaccctga ggaaaggcgg cgcgggccac gcgcttcacc atgtcataga
      301 tgccctggaa attgacttgc ccggtggtgt aagagacggc atccgccggc acgaaagtga
      361 gagaagcagg cgagggctgt ccggcggacc agatgtcaaa gggcgttccg ggaacggcat
      421 cgcccaggat cgcggcctgc acgtgcgttt tcgcgccttc gaacgtgatg tggccgctga
      481 gagagtgcac ggcgtcgagc ttggcagcgt cgaggagcgg gcggacctgg aaggttcctg
      541 ctttcgaatc ggccgcgaat ttttccaggt cgggaatgcg cagaaagaat tccagcaagc
      601 cgctgccaag gttcgcttgc gcttcctgat acgcagcgga ttgcgccagc gatgccgctc
      661 cggaaacttt gccgtcgagg cggccgagta tttcttccat gacggagcgt tcacccgcgc
      721 tgacggcgaa cttgccatgc tcggcccaat acgtggcgcc gcttttgcct tccaccttga
      781 ggacttcgac gcccccgatg gtgacttgcg agaggcgcgg cgcttccttt tcttccgcgc
      841 gcaagcggag aatcgctttc gaaagcaggg cttcttttcc cgtgcggtca tacgcaaaaa
      901 acatgccgtt ccatgccggc ggttttgcat ccgctgaagc agccccgttc gaaacgcttc
      961 gcttcgcagg ctcgctcaaa tacccgatcg tgaatgaatt ttccagaagt gccgcgcatt
     1021 cctggacctg ttcgggtgtg agtttcgttc gatccgattt ttctccagaa gaattaagca
     1081 ttcccgccgc aatcgccgaa cgcatgggag caaaatccgc atcgtcccag agtgcgagga
     1141 gcgagttggc ctttctcgct tctgggccgg gcaccccgtg ccagatcacg tagaacagcg
     1201 tgcgctgcga catttgcgcc ggctccagcg gagcttgcgc gcgtgccgga ttcacccctg
     1261 ccacatacag cagcgctgca atcggcaaga ggatgcgaag gctcagtgct tccatcggag
     1321 gtctccgtga catttggtga agggaaagac taacttttta atgctagcgc ggcgtgcgta
     1381 acgacagaag ccctgtcccg cgctgagttt ccagaacgcg aaacggggct tctccgaagc
     1441 gaaaatcaaa tgaagcgaaa atcaaatcaa acgaaaatcg aaaacgctct gtaattaagg
     1501 tttcgtcgga gtggcggtgg gcgccggtgt cgcggcggcc ggaggagctg cggcgccgcc
     1561 ttccttcggg gtcgcgaggt agccggtaat ttgatttctg ccgacgtcga aaacgacgat
     1621 ttcgtaaggg gccatgcggg agctgccctt cacataaaac tgaacgggct cgccggaggt
     1681 cttgtccttc ttctcgatgg tcttgtcgtc ggcgacgacg gtgactgtgt actttgcttt
     1741 tttcggatcg gttttgttaa gagacatctg cacggggccg acgcgctgcg cgctcttgga
     1801 tctttgcaaa gtgaactcgt agtagttgcg gtcgccccgg cgcttcagat cttccaggtc
     1861 ttcacgactg tgcgcgatca gtccgctcat gacgttcatg tcgcccaagc tgcgttcgag
     1921 tttgcctttg gtagcttcca gatcgttttt ggtggcttcg atgtctttct tggcgccccc
     1981 gacttcg
//
LOCUS       DASYVO010000263         2955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_204704, whole genome shotgun sequence.
ACCESSION   DASYVO010000263 DASYVO010000000
VERSION     DASYVO010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204704"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(378..1289)
                     /locus_tag="VGR03_08715"
     CDS             complement(378..1289)
                     /locus_tag="VGR03_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEV1994399.1"
                     /translation="MKTALSISVIFFFGTLAPAARAQQAKPATAAPATAAPSPTQKSI
                     EAYLRHLYAFGPDVLLVVGPLKETPVEGLLETTIDLTIGDNKEVVKFYVSRDGKFLFR
                     GELSDMTKDPLAENRAQIQMKDAPSMGDSKAAVTLVEYSDFECPVCRSLHDALRGMLP
                     NYAGKVRLVFKDFPIEQLHPWARTAAIAGRCAYQQDPQAFWKMYDLIYDNQEIISAAN
                     AWTKMMDYAGQSGLDADTFKTCMASSQAGAAVNDSRANGQQLDVNSTPTVFVNGRRLV
                     GADAHLLEQYIKYELAQQKSGKTTDKK"
     gene            complement(1305..2942)
                     /locus_tag="VGR03_08720"
     CDS             complement(1305..2942)
                     /locus_tag="VGR03_08720"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEV1994400.1"
                     /translation="MKIALAQFNPTVGDFAGNAARILNLAGQAKQRGADLAVFTELCL
                     CGYPPQDLLERPAFLDRNMDELKALAAKIPLPGLVGYAGRVKNGAGKSVANKAALLCG
                     GRVLFEQSKMLLPTYDVFDESRYFQPADKQVVYSFEDEKLGITICEDVWNDPNFWPTR
                     LYERDPVVELGKQGISVLLNLSASPYTIDKRNLRIEMLRAIAMKQQCPVVYVNQVGGD
                     DNLIFDGASMAITADGRVAAQALAFREDLVLFDTVTGQGEIHEQPPTEIAYAYEALLT
                     GTADYVRKCGFKKVLVGLSGGIDSAVVASIAVDALGAENVLGVSMPGPFSSAGSKSDA
                     ATLAKNLGIEFITLPIASVFEEYKRALAPAFGDRPVDVTEENIQARIRGNYLMALSNK
                     FGSMVLSTGNKSELSVGYCTLYGDMAGGLAVISDVPKLMVYELARWINRERELIPQST
                     MAKAPSAELRPDQKDEDSLPPYEVLDRILKAYIEDLRSPQEIADQCGFDLKLVRDIAL
                     LVDRNEYKRKQAAPGLKITSRAFGFGRPFPIAQKFIP"
BASE COUNT          614 a          899 c          808 g          634 t
ORIGIN      
        1 ctgcagaaca agacgagcgt ttcgatcact gcgcccaacc cgcccagcat ttaaacacgg
       61 gactctccaa tcggtagcaa cgcgccccgc ccatcgattt gcagttccac ggaagcaact
      121 tcacgcgctt ttgacgaaat gaggcccaat tccgcgaccg gctggcgcac cccccgcgcc
      181 agccggaaga acaccccatg gagggccccc aacccatatc aaggacgagc cctatttcca
      241 cagaaaaacg gcaggaagcc gcagattcct cttgacaatg cgtcctataa gtaatattat
      301 gttaactaag aaatcgggct ggatcctcac cgcctccagg tccctgcccg gactcattcc
      361 agcctcctcc ctctctccta cttcttgtcg gtggttttcc cggatttttg ttgggcgagt
      421 tcgtacttga tgtattgctc gagcagatgc gcatcggcgc ccacgagtcg ccgcccattc
      481 acgaagaccg taggcgtgct gttcacgtcg agctgctggc cgtttgcgcg gctgtcattg
      541 acggccgcgc ctgcttgcga gctggccatg cacgtcttga acgtatcggc gtccagcccg
      601 gactggcctg cgtagtccat catttttgtc caggcgttcg ccgccgaaat gatttcctga
      661 ttgtcgtaaa tcaagtcata catcttccaa aaagcctggg ggtcctgctg gtaggcgcaa
      721 cggccggcga tggcagcggt tcgtgcccac ggatgaagct gctcgatcgg aaagtccttg
      781 aagaccaggc gcaccttgcc ggcatagttc gggagcatgc cgcgcaatgc gtcgtgaaga
      841 ctgcggcaga ccgggcactc gaagtccgaa tattccacca gcgtaaccgc ggccttggag
      901 tcgcccatcg aaggcgcgtc tttcatctga atctgcgcgc gattctcggc gagggggtct
      961 ttcgtcatgt ccgacaattc accgcggaaa agaaacttgc cgtccctgct cacgtaaaat
     1021 ttgaccactt ccttattgtc gccaatggtc aggtcgatcg tggtttcgag cagcccttcg
     1081 accggtgttt ctttcagcgg cccgaccact agcaggacat ccgggccgaa ggcgtacaga
     1141 tgtcgaaggt acgcctcgat gctcttctgt gttggcgatg gcgcagcggt cgccggtgca
     1201 gctgtcgccg gcttggcttg ctgcgctcgc gcggcgggcg caagcgtgcc gaagaaaaag
     1261 attacggaaa tgctcagtgc tgttttcacg aggacctcat ggatttaagg gatgaacttc
     1321 tgcgcgatcg ggaatggcct gccaaagcca aatgcgcgtg aggtaatctt caatccgggg
     1381 gcggcctgct tgcgtttgta ctcgttgcga tccaccagga gcgcgatgtc gcggactagt
     1441 ttaagatcga agccgcattg atcggcaatc tcttgggggc tccgcaagtc ttcgatatag
     1501 gccttcagaa ttcggtccag gacctcgtaa ggcgggaggc tgtcctcgtc cttctggtca
     1561 ggacgcaact cggccgaagg agccttcgcc atggtcgact ggggaatcag ttccctctcg
     1621 cggttaatcc agcgcgccag ttcgtacacc atcaatttcg gcacatcgga aatgacggca
     1681 agacctccag ccatgtctcc atagagcgtg cagtagccca ccgagagttc tgatttattt
     1741 ccggtgctga gaaccatcga tccaaactta ttggagagcg ccatcagata atttccgcgg
     1801 atgcgcgcct gaatattttc ttccgtcaca tccacgggcc ggtcgccgaa cgcgggtgcc
     1861 agcgcccgct tgtattcttc aaaaacactc gcaatcggca aggtgatgaa ctcgatgccc
     1921 agattcttcg ccagagttgc cgcgtcactc ttgctgcctg ccgaagaaaa aggtcccggc
     1981 atggaaaccc cgaggacatt ttccgccccc agtgcgtcca cggcgatcga ggcaaccacg
     2041 gcagaatcaa tgccgccgct caagccgacg agaactttct tgaacccgca cttgcgtacg
     2101 tagtctgccg ttccggtcag cagcgcctcg taggcatagg cgatttcggt cggtggctgt
     2161 tcgtgaatct cgccctgccc tgtgaccgtg tcaaagagga ccagatcctc ccggaacgcc
     2221 agcgcttgtg cggccacacg cccatcggcg gtaatcgcca tgctcgcacc gtcaaaaatc
     2281 agattgtcgt cgccgccaac ttgattgacg tagaccacgg gacactgctg cttcatcgcg
     2341 atggcgcgca acatctcaat gcgtagattt ctcttatcga tcgtgtaggg cgaagcggaa
     2401 agattcagca ggacgctgat tccctgcttg ccaagttcca ccaccggatc acgctcgtac
     2461 aagcgcgtag gccaaaagtt cgggtcgttc cacacatcct cgcaaatggt gatgccaagt
     2521 ttttcgtcct cgaagctgta gaccacttgc ttatcggcag gctgaaagta gcgcgactcg
     2581 tcgaacacat cgtaggtcgg cagcaacatc ttgctctgct caaagaggac tcgccctccg
     2641 cacagcagtg ccgctttgtt ggccacggac ttgcccgcgc cattcttcac acgcccggcg
     2701 tagcccacaa ggcccggcag aggaatcttg gccgcaagcg cctttagttc atccatgttc
     2761 cggtcaagaa aggccgggcg ctccagaagg tcctgaggag gataaccgca gagacaaagc
     2821 tcggtaaaaa cagcaaggtc cgcgcctcgc tgcttggctt gccccgccaa gttcaaaatg
     2881 cgagcggcat tccctgcgaa gtcgccaacg gtgggattga actgcgcgag cgctattttc
     2941 atgcccggac tttag
//
LOCUS       DASYVO010000264         4317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_91635, whole genome shotgun sequence.
ACCESSION   DASYVO010000264 DASYVO010000000
VERSION     DASYVO010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91635"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="VGR03_08725"
     CDS             <1..1307
                     /locus_tag="VGR03_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEV1994401.1"
                     /translation="KLADVMAPAIRLAEDGFAVSAKLAHELEEERPRLENFQASKRIF
                     LNGEKMWKAGDRLKQAELAVTLKRIARNGAAEFYRGETAQMLVSDMSKNGGLVSLEDL
                     AHYKVKVREVLHAKYESGGHNWEVITAPPPSSGGVAVIEALNMLQGVPLKGWNDPQSV
                     HMVAETMRRVFADRAAYLADPDFSDVPVAGLTAPCYAKERAATIDPANASSSTKVKAG
                     DLRACGSSSNNASVPQTIVSLGEGPHTTHFSVVDAAGNAVSSTYTLNDSYGSHVTCSA
                     GFMLNDEMDDFTTQPGVPNALYGLMQSDANAIAPGHRPLSSMTPTILLRDGKLSFVTG
                     SPGGPTIISATLLSVINWMRLGMEPQAAINAPRFHHQWLPDRILMESAFPSTLESALN
                     ARGHETKRLAVIGLVNAIGIDPQTNERLGTADPRDEGSAVGY"
     gene            1363..2226
                     /locus_tag="VGR03_08730"
     CDS             1363..2226
                     /locus_tag="VGR03_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEV1994402.1"
                     /translation="MQRLLSTYLFVSRKLTPELLGQIASAGFQGVEIFCSRGHFDYTA
                     RTEMQAMAGALAQHGLILSSMHAPVSRDISSTREGGTPLSICEVERVRRIEAMDELKR
                     AIDVAEELSFPRMVFHMGSTRETADPRKRDAAFSSLEHLILHARHLGVAICVENTTSE
                     MGDPSYLRAFVDETRLTGLRFNFDVGHAHLAELPEDERLEKSFSPLRELVSSVHVHDN
                     HGEKDEHLPPFDGTIDWPAAIKLLKTAPEANLPLVLELKEKTGPEAPSVVEHLAAARK
                     SMDRFEIAWSA"
     gene            2347..2586
                     /locus_tag="VGR03_08735"
     CDS             2347..2586
                     /locus_tag="VGR03_08735"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEV1994403.1"
                     /translation="MIEVAISEFKAKCLALLEQVRRTRQPIRITRHGKPVAEVVPPTA
                     LQDRASWIGSMKDSKIIGDIISPADDEDEWEALRD"
     gene            2583..2969
                     /locus_tag="VGR03_08740"
     CDS             2583..2969
                     /locus_tag="VGR03_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEV1994404.1"
                     /translation="MKLLLDTHVWLWLMIDPQRLGRSTLRALKDEQNELWLSPISTWE
                     ALTLHHKGRVNLHGELTAWVAQATAGMKEAVLTHEIMIAARQLSLHQDPSDRMLAATA
                     LVLDLTLVTADERLLGLGTVRTLADR"
     gene            3152..>4317
                     /gene="asnS"
                     /locus_tag="VGR03_08745"
     CDS             3152..>4317
                     /gene="asnS"
                     /locus_tag="VGR03_08745"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003037.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV1994405.1"
                     /translation="MTTITIENAGKHDGQEVTIKGWLYNLRESGKLLFPIFRDGTGII
                     QGVCTLKDNPEAFESLKGLTQESSVIATGKIRAEKRAPGGYEIGVIKIQIVQKVSESA
                     PFPIQLKEHGVDFLLDQRHLWIRTPRQAAILRIRAEAIRAAREFMDGQGYTLTDAPMF
                     TPAACEGTSTLFEVNYIDDQKAYLTQSGQLYAEAIAMALGKVYTFGPTFRAEKSKTRR
                     HLTEFWMLEPEAAYVEFDEAMSLGEGLVSAIVQSVLRNKPRELETLKRDTSKLENVKP
                     PFPRISYEEAVAVLNKNGNPAKFGDDFGGDEETIISSSFDRPVLIHHYPSSIKAFYMQ
                     PDAQRPELALAFDMIAPEGYGEIIGGSQRIHDYDLLLKRLREHNLPEESFQWYL"
BASE COUNT          974 a         1314 c         1187 g          842 t
ORIGIN      
        1 tgaaactcgc ggacgtgatg gccccggcaa ttcggctcgc ggaagacgga tttgcagtca
       61 gtgcaaagct ggcgcatgag ctggaagaag agcggcccag gttagaaaat tttcaagcta
      121 gcaaacgcat ttttttgaac ggcgaaaaaa tgtggaaagc cggagacagg ttgaagcagg
      181 cagagttggc ggttacgctg aagcgcatcg cgaggaacgg cgcggcagaa ttttatcgcg
      241 gtgaaacagc gcagatgctg gtcagcgata tgtcaaagaa cggcggactc gtctccctgg
      301 aggatctcgc acactataaa gtgaaggttc gcgaagtctt gcatgccaaa tacgaaagcg
      361 gcgggcacaa ctgggaagtg atcactgcgc cgccgcccag ctcgggcgga gtcgcggtga
      421 tcgaggcgct gaatatgctg caaggcgtgc cgctgaaagg ctggaacgat ccgcagagtg
      481 tccacatggt tgctgaaacc atgcggcggg tttttgccga ccgtgcagcg tatctcgcgg
      541 atcctgattt ttctgacgtg ccggtcgccg ggctgacggc cccgtgctac gcaaaggagc
      601 gtgcggccac cattgatccc gcgaatgcgt catcaagcac aaaagtgaaa gctggcgatc
      661 tacgtgcatg tggaagttct tcaaacaacg cttccgtgcc acaaacgatc gttagtttgg
      721 gcgaggggcc gcataccacg cacttttcgg tcgtggacgc cgccggaaac gcggtgtcca
      781 gcacgtacac actgaacgac agctatggtt cccacgtcac gtgctccgcg ggatttatgc
      841 tgaacgatga gatggacgac ttcactaccc agccgggcgt gccaaacgcg ctgtacggcc
      901 tgatgcaatc cgatgcgaac gccatcgcgc caggacatcg cccgctcagc tcgatgacgc
      961 caacaattct tctccgcgac ggcaagctca gcttcgtcac gggttcgccg ggcggaccga
     1021 cgattatttc cgcgacgctg ctcagcgtga tcaactggat gcgcctggga atggaaccgc
     1081 aggctgccat caacgctcca aggtttcatc accagtggct gcccgaccgc attctaatgg
     1141 aaagcgcatt cccctccact ctggaaagtg cgctgaatgc acgcggccat gaaacgaagc
     1201 gccttgccgt catcggactt gtgaacgcca ttggaatcga tccgcaaaca aacgagcggc
     1261 tgggcacagc cgatccgcgc gatgaagggt ccgctgtggg atactagtga agataagtcc
     1321 gttcaggttg cggacgctcc aagctggaaa tccaacaaga tcatgcagag actcctgtcc
     1381 acttacctgt tcgtttcgcg aaaattgacg cccgaactcc tcggccaaat cgccagcgcc
     1441 ggattccagg gcgtcgagat cttctgctcg cgcgggcatt tcgattacac cgcaagaacg
     1501 gaaatgcaag cgatggctgg cgcacttgcc cagcacggac taatcctttc ctcgatgcac
     1561 gctccggtga gccgggatat tagctcgacc cgcgaaggtg gcacgccgct ttcgatctgc
     1621 gaagtggagc gcgtgaggcg gatcgaggcg atggatgaat tgaaacgcgc tatcgatgtg
     1681 gcggaagaac tgtcgtttcc gcgcatggtg tttcacatgg gcagcacgcg ggagacggcc
     1741 gatccgcgga agcgcgatgc ggcgtttagt tcgctggaac atctcatact gcatgccagg
     1801 cacctgggcg tcgcgatttg cgtcgagaac accaccagcg aaatgggcga tcccagttat
     1861 ctccgcgcct tcgtcgacga aacccgcctc acaggcctcc gcttcaactt tgacgtcggc
     1921 cacgcccacc tcgccgaatt gcccgaagac gagcgcctcg aaaaaagttt ttctcccctg
     1981 cgcgaactcg tctcttccgt ccacgtccac gacaatcacg gcgagaagga tgagcacttg
     2041 ccgcccttcg acggcaccat cgattggcct gccgccatca agctcctaaa gaccgcgccg
     2101 gaagcaaact tgccgctggt gctggaattg aaggaaaaga caggtccgga agccccctca
     2161 gttgtcgagc atctcgccgc cgcccgtaag tccatggatc gctttgagat agcctggtcc
     2221 gcctaacgcc cctcggtttt gctcataacc ggcggtatct gcgcttgctc ccctggagat
     2281 cgcgccaatg ctacatattg accattattc tgactatggt aagattattg gccagaaagg
     2341 acaacaatga tcgaagttgc catctcggaa ttcaaagcca agtgtctggc gcttctggaa
     2401 caggtgcgcc gcactcgcca gcccattcgc atcacgcgtc acggaaaacc cgtcgccgag
     2461 gttgttcctc ctactgctct tcaagaccgg gcctcctgga ttgggtccat gaaggactcc
     2521 aagattatcg gagacataat ttctcccgcc gatgacgagg acgaatggga ggcgttgcgc
     2581 gattgaaatt actgctagat acgcatgtct ggctctggct tatgatcgat ccccaacgct
     2641 tgggacggag caccttaaga gcactaaagg acgagcagaa cgaactctgg ctttcaccca
     2701 tcagcacatg ggaagctctc actctgcacc ataaaggaag ggtcaaccta cacggcgaac
     2761 tcaccgcatg ggttgcccag gcaaccgcag gcatgaagga agctgtcctg acccatgaaa
     2821 tcatgatcgc agcgaggcag ttgtccctgc atcaagatcc ctccgaccgg atgcttgcag
     2881 ccacagcgct tgtcttggac ttgacgctcg tcacggccga cgaacggcta ctaggtctag
     2941 gaacggtgcg aactctagca gatcgctgag ttgcctgagg aaggcgcggt gttgagccgg
     3001 cttccgagtg tccgacttcg cagccacttg gatctggagg gctgcctggc attgcattgt
     3061 gagtgggcgc gagaaacgcc cgtttatgtg atcttgcctg tcctcgtctt tgctaaactc
     3121 gtcgtctgaa tcttcatcag agaagctcac tatgaccacg attacgattg aaaacgcagg
     3181 caagcacgac ggccaggaag tcactatcaa gggctggctc tataacctcc gcgaatccgg
     3241 caaattgctc ttcccaatct tccgtgacgg caccggcatc atccaaggcg tctgtacgct
     3301 caaggacaat cccgaagcgt tcgaatcgct gaagggcctc acgcaggaat ccagcgtcat
     3361 cgccaccggc aaaatccgcg cggaaaaacg cgcccccggc ggctacgaga tcggcgtcat
     3421 caaaattcag atcgtccaga aagtctcgga gagcgcgccc ttccccatcc agctcaaaga
     3481 gcatggcgtc gattttctgc tcgatcagcg ccatctctgg attcgtacgc cccgccaggc
     3541 cgccattctc cgcattcgcg ccgaagccat ccgggccgcg cgcgaattca tggacggcca
     3601 gggctacacg ctcaccgacg cgcccatgtt cacccccgcc gcctgcgaag gcacctccac
     3661 gcttttcgag gtcaattaca ttgacgatca aaaagcctac ctgacgcaga gcggtcagct
     3721 ctatgccgaa gccatcgcca tggctcttgg caaggtctac accttcggcc ccaccttccg
     3781 cgccgaaaaa tccaagaccc gccgccacct caccgagttc tggatgctcg agcccgaagc
     3841 cgcttatgtc gagttcgacg aggccatgtc cctcggcgaa ggtctcgtca gcgccattgt
     3901 gcaatccgtc ctgagaaata aaccgcgcga acttgaaacg cttaagcgcg acacctccaa
     3961 gcttgaaaac gtgaagcccc cgttcccgcg catcagctac gaagaagccg tcgcggtttt
     4021 gaacaaaaac ggcaatcccg ccaagttcgg cgatgacttc ggcggcgacg aagagaccat
     4081 catttccagc tcgttcgatc gcccggtcct gatccatcac tatccttcgt cgatcaaagc
     4141 tttctacatg cagccggacg cccagcgccc cgagctcgcc ctcgcctttg acatgatcgc
     4201 ccccgagggc tacggcgaaa tcatcggcgg cagccagcgc attcacgatt acgatctgct
     4261 tctgaagcgc ctgcgcgaac acaacctgcc ggaagaatcc ttccagtggt atctcga
//
LOCUS       DASYVO010000265         5030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_34964, whole genome shotgun sequence.
ACCESSION   DASYVO010000265 DASYVO010000000
VERSION     DASYVO010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34964"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..711)
                     /locus_tag="VGR03_08750"
     CDS             complement(121..711)
                     /locus_tag="VGR03_08750"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV1994406.1"
                     /translation="MKNKKPNAEHIWKQFEDLLVPRLRLSVIERAVYSHLLRHSRLEG
                     RLRLRFSIPWLARGVRLCQMTARCALRRLFDLRVLRLVERNQVGHVVVVRLPKEIRAL
                     RPYIIAARGAARLPLAASIADADFFKNKALRQAIHSRERSLCFYCLRRLTVLVRTIDH
                     VIPRAQSGRNSYRNLVSCCRDCNSKKGQTPAEDFLR"
     gene            complement(1374..2153)
                     /locus_tag="VGR03_08755"
     CDS             complement(1374..2153)
                     /locus_tag="VGR03_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV1994407.1"
                     /translation="MPINTIIVDDEKPARDELAFLLKGFPEINVIGQGKNGVEAVSLI
                     KEHAPDLVFLDVKMPGLDGFGVLKKIVERKMKMPHVVFATAFDQYAVQAFDVNAVDYV
                     LKPFDKARIAKAIARARKMLETHTSASERLEQLVSQLGAAKQSSSPPVKVLVKSQQRL
                     LLVDSEDLVFASIDRGTITVMSRDAEGTSNYHTLEEMQAALDSESFWRPHRSFLVNIH
                     HIKEVVPWFNSTYVLKMNDKKHTEIPVSRSQISRMRELFKL"
     gene            2337..2897
                     /locus_tag="VGR03_08760"
     CDS             2337..2897
                     /locus_tag="VGR03_08760"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1994408.1"
                     /translation="MKKSSGTPRAKKARVLSSATIYEGPVFGIRRDEVIEPSGVRTTR
                     EVITHPGSVVVLPVLPDGRILLIQQYRHATRQYLWELVAGRMDGGETPKAAAARELIE
                     ETGYRAKRFRVFLDVFPTPGFLEERMFILLAEGLTAGEAEPEEDEKIISRAYNRKQLE
                     EMIRSGKLRDAKSIAGILYYLRFLCE"
     gene            3100..3303
                     /locus_tag="VGR03_08765"
     CDS             3100..3303
                     /locus_tag="VGR03_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEV1994409.1"
                     /translation="MQGTVKWFNNSKGYGFIGRDDGPDVFVHYSAIVGDGYRTLQEGD
                     PVEFEIVQGPKGPQAANVSKAGG"
     gene            3436..>5030
                     /gene="polX"
                     /locus_tag="VGR03_08770"
     CDS             3436..>5030
                     /gene="polX"
                     /locus_tag="VGR03_08770"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEV1994410.1"
                     /translation="MDNKQIARVLRETAQLLEIDGAIIGRYRSYEKAAELIDGLPESI
                     EQLVKEPEKLKELPGIGDRMVEHLQEIVKTGDYALRKKLLKKYPATILDLLQLQSLGP
                     KKVAFLWSNFKAGTVADVERIAKEGKLRDVAGFGEKSEQNILKAVEVFKKSSGRFHIH
                     TAEDAAAAVISHIEKAGKAVESVTPAGSLRRGKETVGDLDLLLTLADGHTSQKHVDAL
                     AKHILAFPGIDQTLAHGENKVSFTLENGLQVDVRLLEKENFGATLLYFTGSKEHNVAL
                     RGRANDMGLTLNEYALATLKGEKRVAGRTEEEIYAKLKLDFIPPELRENTGEIAAAEN
                     HKLPHLLTLKDMKGDLQMHTTASDGKNSIEEMAEAARELGHHYIAITDHSKAVTVANG
                     LDEKRMAAHIKKIHAANDKDLGIRVLAGAEVDILKDGSLDYSDELLAQLDVVVCSIHS
                     YFNLDRAAMTERMLAAIENPYTQIIAHPTGRLLLRRDPIDYDMEKVLEACAKHGVAME
                     CNSYPDRLDLKDVYLRMCKERGV"
BASE COUNT         1178 a         1462 c         1449 g          941 t
ORIGIN      
        1 gcgagggaga gggctggcgg ctgtgggcag cgaagggggg agcttgccgg aggctagggc
       61 gtcgagggcg cggaggcggg cggtgagttc ggcgtcggtg aggcggcgct ggcggaagag
      121 ctagcggagg aagtcctcgg cgggggtctg gccctttttc gagttgcaat cgcgacaaca
      181 agaaacgagg ttgcggtagg aattgcgccc cgactgcgca cgagggatga catggtcgat
      241 ggtgcgtacg aggacggtaa ggcggcgcaa acagtaaaag cagaggctac gctcgcgcga
      301 gtggatggcc tggcgcagag ccttgttctt gaagaaatcg gcgtccgcga tgctagcggc
      361 gagaggcagc cgggccgcgc cgcgggcggc gatgatatag gggcggaggg cgcgaatctc
      421 cttaggcaag cggaccacga ccacgtgacc gacctggttg cgttcgacca agcgcaagac
      481 gcggagatcg aagaggcgtc gcagggcgca acgcgcggtc atttggcaga ggcggacacc
      541 acgggcgagc cagggaatgg aaaagcggag gcggagcctc ccttcgaggc ggctgtggcg
      601 caggagatga gaatagacgg cgcgctcgat gaccgagaga cgcaggcgcg ggacgagaag
      661 atcttcgaat tgtttccaga tatgttcggc gttgggtttt ttgtttttca tggtttgggt
      721 ccagagcacg caggcgcggg tgcgcgcgcg gcgaagggag atttgggaca ccagagcaaa
      781 aggagaagtt gacagtcccg acacggtcgg gatcttcgat cgacagttaa cgggcaaaag
      841 aagggacaaa gacttaacgc agagagcacc ccgacccggt cggggtaaac gccgccagca
      901 gcgatcacag agaaaaaaga aaaaagcaga tccctcgctg cgctcgggat gacaatttcg
      961 cgctgaccct gcctgcggca tgcaggcgcg ggagcaggga cacggcggct gcgaccctag
     1021 gagggccagt acggtctaac gttgagtttt cagcaggctg aaatctagag cgatccgggg
     1081 caggctaccc gcaaaaagga aaagggcgag ttgtgtgaac cacaactcgt cctacaaata
     1141 ggataccaga tcggaagcat tttgcaagag ctatttttgt cttgcgacac agtcctcaga
     1201 cggcgcgact gacgagcggc cgcacctccg cctccatgaa ttggcggacg gtatcgcgta
     1261 tcagtttttg gtggtcgtta agcgaaaaat ccatcgtctc gtcgcacgcg gggtacgcac
     1321 gatgctagca cactcagtca cagctctagt agcaccgcgc gtcggaatca cggctacagc
     1381 ttgaagagtt cgcgcatgcg gctgatctgc gagcggctca cgggaatttc cgtatgcttc
     1441 ttgtcgttca ttttcagcac gtacgtggag ttgaaccacg gcacgacttc cttgatgtgg
     1501 tgaatattca ccaggaacga gcgatgcggc cgccagaacg attccgaatc cagcgccgcc
     1561 tgcatctctt cgagcgtatg gtaattcgac gtgccctcgg catcgcgcga catcaccgtg
     1621 atggtccctc ggtctatcga ggcaaacacc aaatcttcac tgtccaccag caacagccgc
     1681 tgctgcgact tcaccagcac cttgaccggc ggcgaggaac tctgtttcgc cgcacccagc
     1741 tggctgacga gttgctccag ccgctccgaa gccgaagtat gcgtctccag catcttccgc
     1801 gcgcgcgcaa tcgctttggc gatgcgtgcc ttgtcgaacg gtttcaacac gtagtccacg
     1861 gcattcacat caaaggcttg cacggcgtat tggtcgaacg ccgtggcaaa aaccacgtgc
     1921 ggcattttca tctttcgttc gacgatcttt ttcaacacgc cgaacccatc gaggccgggc
     1981 atcttcacat ccaagaacac cagatccggc gcgtgctctt taatcaacga aacggcctcc
     2041 acgccgttct tcccctgccc gatcacattg atctccggga accccttcaa caggaacgcc
     2101 agctcgtctc gcgccggctt ctcgtcgtcc acaatgatcg tgtttatcgg cattttcttt
     2161 gcttctaagt ggggccgagc ttcaacgcgc tcaagtcgat tgcagcactc gcgttgaagc
     2221 cgagtatagg cgcaccccgg ctccgcaagc aaacaacact ggggcctggc gcggcgtggc
     2281 gaatttcccg tttcccggta tcatgggcca gaagaaacag agccaggaca acaaaaatga
     2341 aaaaatcttc aggtacgcca cgcgcaaaga aggcaagagt cctcagcagc gccacaattt
     2401 atgaaggacc ggtcttcggg attcggcgcg acgaggtcat cgagccgagc ggcgtgcgaa
     2461 cgacccgcga agtcatcacc catccgggtt ccgtcgtggt ccttcccgtt ctccccgacg
     2521 ggagaatcct cctgattcag cagtaccgcc acgccacgcg gcagtatcta tgggagttag
     2581 tcgccggccg catggatggc ggggagactc ccaaagcagc cgcggcccgc gagctgattg
     2641 aggaaaccgg ttatcgcgca aagcgctttc gagtcttcct ggacgttttt ccgacccctg
     2701 gtttcctgga agaacgcatg ttcatcctcc tcgcagaagg tctgaccgca ggcgaagctg
     2761 aacccgaaga agacgagaaa ataatttccc gtgcctataa ccgtaagcaa ctggaggaga
     2821 tgatccgcag cgggaaactt cgcgacgcca agtccatcgc cggaatcctc tattacctgc
     2881 gcttcctttg cgaataaccg cgtttcacaa tgtgaacttt tgaatcgttt ttatacaggg
     2941 ataagtctgc ttttttctag cagggaggac gattgttcta ttgactcgga aacccattct
     3001 gaatacactc cgcgcaattc gtatctttaa cagcgttagg gttattcgcc tcgcgctgcg
     3061 gagcgaaatc tcgaagtccc tgtcaggagg gcaagcgaag tgcaaggaac cgtgaagtgg
     3121 tttaacaatt ccaaggggta tggcttcatc gggcgcgatg atggtccaga cgttttcgta
     3181 cactattccg ccatcgtcgg tgatggttat cgcaccttgc aagaaggaga tccagtcgag
     3241 tttgagatcg ttcaaggccc caaaggtccc caggccgcca acgtctcaaa ggccggtggc
     3301 taaagcagtt agcaataatt tggccggcag acgcgcccaa ctgccggcct ttcttatttg
     3361 tggatgctcc cccttacttc cataccgcca acacgctgcc acttgctccc cgccctggct
     3421 tataatcggc ataccatgga caacaaacag atcgcccgcg tgctccgcga aaccgcccaa
     3481 cttctggaaa tcgatggcgc cattatcggc cgatatcgca gctacgaaaa ggccgccgaa
     3541 ctgatcgacg gcctgcccga atccatcgag caactcgtca aggaaccgga aaagctcaaa
     3601 gaactcccgg ggatcggcga ccgcatggtc gagcatctgc aggaaatcgt taagaccggc
     3661 gattacgcgc tgcgcaagaa gctgctcaag aaatatccgg cgactatcct ggatttgctg
     3721 caactccagt ctcttggccc gaagaaggtc gccttcctgt ggtccaactt taaggccgga
     3781 accgtggccg acgtggagcg aatcgcaaaa gaaggaaaac ttcgcgacgt cgccggcttc
     3841 ggcgaaaaaa gcgaacaaaa tatcctcaaa gcagtggaag tcttcaaaaa atccagcgga
     3901 cgatttcaca tacacaccgc cgaagacgcg gcggccgcgg tcatttcaca cattgaaaag
     3961 gcaggaaagg ccgtcgaatc cgtcacgccc gcgggctcgt tgcggcgggg aaaagagact
     4021 gttggcgatc tcgatcttct tctcaccctt gccgatggcc acacgtcgca gaaacacgtg
     4081 gacgcgctag ccaagcacat cctggcattt cccggtatcg accaaacact ggcgcacggg
     4141 gaaaacaagg tcagcttcac gctggagaac ggattgcaag tcgatgtccg tctcctcgaa
     4201 aaagaaaact ttggcgctac cctgctctat tttaccgggt cgaaagagca caatgtggcg
     4261 ctccgcggcc gcgccaacga catgggactc acacttaatg aatatgcttt ggctacgctg
     4321 aagggtgaga aacgcgtcgc cgggcgcacc gaagaagaga tctacgcgaa gctcaagctg
     4381 gattttatcc cgcccgagtt gcgcgagaac accggcgaaa tcgccgccgc cgaaaaccac
     4441 aaactgccgc atctgctcac cctgaaggac atgaagggcg acctgcagat gcacaccacg
     4501 gccagcgacg gaaagaactc gattgaagaa atggcggaag ctgcccggga actcggccac
     4561 cactacatcg ccatcaccga ccattcaaag gccgtcaccg ttgccaacgg cctggacgaa
     4621 aagcgcatgg ccgcccacat caagaaaatt cacgccgcca acgacaaaga cctgggcatc
     4681 cgcgttctcg ccggcgccga agtcgatatc ctcaaggatg gctcgctcga ctattccgac
     4741 gaactccttg cgcaactcga cgtcgtggtc tgctcaatcc actcctattt caacctcgac
     4801 cgcgcggcca tgacggagcg catgctcgcg gccatcgaaa acccctacac gcagatcatc
     4861 gctcacccta ccgggcgcct gcttctccgc cgcgatccca tcgactacga catggaaaag
     4921 gtgctcgagg cttgcgctaa acacggcgtg gctatggagt gcaactccta tcccgaccgg
     4981 ctcgacctga aagacgtcta tctccgcatg tgcaaggagc gcggcgtcaa
//
LOCUS       DASYVO010000266         4176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_134011, whole genome shotgun sequence.
ACCESSION   DASYVO010000266 DASYVO010000000
VERSION     DASYVO010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134011"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VGR03_08775"
     CDS             <1..895
                     /locus_tag="VGR03_08775"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1994411.1"
                     /translation="GTVLLKAGNRPASQVAVKLKSHAAGIFRSILTDLEGHFEVRSLP
                     PSTYEIVVDEPGYEPAQTSTQLDGSSSKLVLYLNSSNLPQTERNSYTVSTRELKIPGK
                     ARSEYEKGLGSLTKKDLAGSLSHFTKAAQAFPDYYEAYYHTGVAETGLGRLDEAMQAF
                     QKAIDLSGGRYAWAEFGFGYLLHLEGKSEEAVTIIRRGLEVDASSPDGYLVLGMAQLR
                     LNRLDEAERSAREALLRNPNFAQAYLVLSDTYARRHEYRAQLQGLDAYLKLEPSGPES
                     DHVRQAREVVLRILAKHPPEN"
     gene            complement(1006..2349)
                     /locus_tag="VGR03_08780"
     CDS             complement(1006..2349)
                     /locus_tag="VGR03_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV1994412.1"
                     /translation="MPELVRTLRLRDLLLLFIGSVIGSGIFRTPGPILRQVDGSVGLS
                     LLVWIVGGVLSLLGALTYAELAAANPEAGGLYCYIRDGFGRLAAFLYGWCLFLVIASG
                     SVAALARAFSEYLARVLPISPGQQTFAAVVMIGIVTAINVWGTRKSSDLQNWTTLIKV
                     GIIVLLSALLLSLGHHGRDIPSALGAAQHGRGLWSNFGLAMITVLWAYEGWQFGTYSA
                     GEVVDPQRAFPRAFLMGSLILVGLYLVAIVAYLFALGPADMAASDTIAATSVAVVLGP
                     WAAKIVALTILISTFSAANSVILTAPRVFYAMANDKLFFKKLAEVHPRFKTPAAAIIG
                     LGVWSAVLVCAGELGTFSKLIGGVIFIGWIFYGLGAAAIFPIRRASAGRPIPYRVPGY
                     PWTPMLFVLVAAAIVGNAIYLAFRDPSQFMNLAVAVLLLLIGLPAYFFWRKRAAS"
     gene            complement(2463..3473)
                     /locus_tag="VGR03_08785"
     CDS             complement(2463..3473)
                     /locus_tag="VGR03_08785"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV1994413.1"
                     /translation="MSTPNPENPPPSIPINIDWRAIPFGQAPYTPVPPRPSLKMLFIA
                     GVLFLLTLCTCLVAGTQFAVAYAQNQAVSLDEFLNAFTLFYKHPAALAAGLPFALTLL
                     TILLAHELGHFFACRHHHIRASYPFFIPFPSLIGTFGAFILIRSPIRTARALFDVGAS
                     GPLVGFLLAVPALAYGVLHAKIVPGLDDIRNVDLAFGTPLLLRLFTAALHHGISPGTL
                     LLHPVGRAAWVGLFATSLNLLPVAQLDGGHILRSLSARLHRYMSFLLPLILVSLGLFG
                     FWQGWYVWGALLLGMRFLRIAPIYDPTPLDPNRRWGAFFALLVFLLCFMPAPLYFSPS
                     SH"
     gene            complement(3600..3887)
                     /locus_tag="VGR03_08790"
     CDS             complement(3600..3887)
                     /locus_tag="VGR03_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV1994414.1"
                     /translation="MIKLDIVNAVVVATNISRTKAEQAVETVFESLKQALGRGQRIEL
                     RRFGVFSVKPRKTGIGRNPRTGEEVSIPPGKAVRFKPGKELQGIEQEKKAE"
BASE COUNT         1058 a         1048 c         1380 g          690 t
ORIGIN      
        1 cggaacggtg ctcttgaagg caggcaatcg gcccgcgagc caggtggccg taaaactgaa
       61 atcgcacgcg gcggggatat ttcgcagcat cctgacagac ttggagggcc actttgaagt
      121 gcgaagcctc cctcccagca cgtacgagat cgtcgtagat gaaccgggct acgagccggc
      181 acaaacaagc acacagctcg atggctcttc ctcgaagctt gtgctttacc tcaattcatc
      241 caacttgccg cagaccgagc ggaacagtta cacggtttcg acgcgggagc tgaagattcc
      301 cggcaaggct cggagtgagt atgaaaaggg attggggagc ctgacaaaga aagatctcgc
      361 tgggagcctg agtcacttca cgaaggctgc ccaagcgttc cccgattact atgaggccta
      421 ctatcacaca ggcgtggcgg agacgggtct tggccgtctg gatgaggcga tgcaagcttt
      481 tcaaaaggcc atagatttga gtggtgggag gtacgcctgg gcagagtttg gcttcggcta
      541 ccttctccat ctggagggaa aatcagaaga ggcagtgacc atcatccgca ggggattgga
      601 agtggacgcg agctcacccg atgggtattt agtcctgggt atggcgcaac tgcggctaaa
      661 tcgcctggac gaagcggaaa gaagcgcgcg tgaggctctg ctccgcaatc ccaattttgc
      721 ccaggcctat ctcgtgctgt cggatactta cgcacgcaga catgaatatc gcgcgcaact
      781 ccaaggcttg gatgcctatc tgaaacttga gccgagcggc cctgagagcg atcacgtccg
      841 ccaagcgcgc gaggtagttc taagaattct agccaagcat cctcccgaaa actgagtttg
      901 ctgcccacca gaacgctgct aacttatgga caacggttcg cgaagcgcga ccggtgtctg
      961 actacacctt gcgggactaa agtcccgcat ctacaggaac tgagttcaag aagcagcgcg
     1021 tttgcgccag aagaagtagg cggggaggcc gatgagaagc agcaggacgg cgacggcgag
     1081 attcatgaac tgggaagggt cgcgaaaggc gagatagatg gcattgccga cgatggcggc
     1141 ggcgacgaga acgaagagca tcggtgtcca cggatagccg gggacgcgat agggaattgg
     1201 ccggcccgcc gaagcgcggc gaatgggaaa aatggcggcg gcgccgaggc cgtaaaagat
     1261 ccatcctata aaaatcactc cgccgatcaa tttgctgaaa gtgcccaatt caccggcgca
     1321 caccagaact gccgaccata cgcccagtcc gatgatggcc gctgccgggg tcttaaaccg
     1381 ggggtggact tcggcgagtt tcttgaaaaa aagtttgtcg ttggccatgg cgtagaaaac
     1441 acgaggggca gtgaggataa cgctattggc ggcgctgaag gtggaaatca gaatggtgag
     1501 cgccacgatt ttggctgccc atgggccgag gacgacggcg acagacgtgg cggcgatggt
     1561 gtcgctagcg gccatgtccg cggggccgag ggcgaagagg taagcgacga tggcgacgag
     1621 atagagcccg acgagaatca gtgagcccat cagaaaagcg cgcggaaagg ctctctgtgg
     1681 atcaacgact tcgccggcgc tgtacgtgcc gaattgccag ccttcataag cccagagaac
     1741 cgtgatcatc gccaggccga aattggacca gaggccgcgt ccgtgttgag cagcgcccaa
     1801 ggctgaagga atgtcgcggc catgatgacc taaggaaagc agcaaggcac tgaggagaac
     1861 aatgatgcca accttgataa gcgtggtcca gttctgaagg tccgaactct tacgcgttcc
     1921 ccagacgttt atggcagtca cgattcctat catcacgaca gcagcgaagg tttgctgccc
     1981 gggagaaatc ggaagcactc ttgccaggta ttcgctgaag gcgcgcgcca aggcagcgac
     2041 ggagccgctg gcaatcacca ggaagagaca ccagccataa aggaaggcag cgagacggcc
     2101 gaagccgtca cgaatgtagc agtacaaccc acccgcctcg ggatttgctg ctgcgagctc
     2161 cgcatacgtg agcgcgccga gcaatgagag gacgccgccc actatccaaa ccaagagcga
     2221 aaggccgacg gagccatcga cttggcgaag aatggggcca ggcgttcgga agatgccgga
     2281 gccaatgacg gagccgatga agagcagcag aagatcgcga agccgaagtg tgcggacgag
     2341 ttcaggcatg gttgtggtgg cgtgatagcc cccgaatggg cacagcatca agtgcaagag
     2401 tatgccaatc tggagattgg cgttcccggg ccgaaggcat cataaccgga gatgggaagc
     2461 ggtcaatggg aacttggaga aaagtagagc ggcgcgggca tgaaacagag gagaaagacg
     2521 agaagtgcaa aaaacgctcc ccatcggcga ttagggtcga gcggagtggg atcgtaaatg
     2581 ggcgcgatgc gcaggaaacg catgccgaga agcaacgctc cccaaacata ccagccttgc
     2641 cagaaaccaa atagcccgag cgagacgaga atgagaggaa gaaggaagga catgtagcgg
     2701 tgaagccgag cgctgagcga acgcagaata tggccgccgt cgagttgggc aaccggcaag
     2761 agattgagcg aagtggcgaa aagcccgacc caggcggcgc ggccaaccgg atgaagcaag
     2821 agtgtgccgg gggaaatgcc gtgatgcagc gcggcagtaa agagacgcaa caggagcgga
     2881 gtgccaaacg ccaggtcgac gttccgtatg tcgtcgaggc ccggaacaat tttggcgtgg
     2941 agaacgccgt aagcaagtgc gggaaccgcc agaaggaagc ccaccagcgg accggaagcg
     3001 cccacgtcaa agagcgcccg agcggtgcga atcggcgaac gaataaggat gaacgcgccg
     3061 aatgtgccga tgagcgacgg aaagggaata aaaaaaggat aactggcgcg gatgtgatga
     3121 tgacggcagg cgaagaaatg gccgagctca tgggcgagca gaatggtgag caaggtgagc
     3181 gcgaagggca ggccagccgc gagggccgcc gggtgtttgt aaaagagtgt gaaggcattg
     3241 agaaattcgt cgagcgaaac agcctgattc tgcgcgtaag cgacggcaaa ttgagtgccg
     3301 gcaaccaagc aggtgcaaag agttagaaga aataaaacgc ccgcgatgaa tagcatcttg
     3361 agcgaagggc gagggggaac gggcgtatag ggggcctggc cgaagggaat ggcccgccaa
     3421 tcaatattga tcggaatgga tggcggcgga ttttctgggt ttggcgtgct cacgaagtgg
     3481 ccggcgcgac gaaggggatc ggcaaacaac agtacagcat aactgcacgg gttcgcgcca
     3541 gagccgaaac agaaaagggt tcgtgcgatt ggagaggttg agactttgct acggcaggat
     3601 tattccgcct ttttttcttg ttcgatgccc tgaagctctt ttccgggttt gaaacggacg
     3661 gctttgcccg gcggaatgct gacctcttcg ccggtgcgcg gattgcggcc gatgccggtc
     3721 ttgcgcggct tgacgctgaa cacgccgaag cggcgaagtt cgatgcgctg gccacggccg
     3781 agagcctgct tcaacgattc gaaaacggtc tcgacggcct gctcggcttt ggtgcggctg
     3841 atgttcgtcg cgacgacgac ggcatttacg atgtccagct tgatcacgca atcctcccgc
     3901 gcttccgagg cagatgctag agggtgcggg gcaggttgtc aagatgggag gggtaatcta
     3961 cactgaacat cctttggcgc tctcgttcga atcactttgg tgcagcaacg aatcgaagag
     4021 acaaatcgca ggtacatttt ggggaggaat ggatggccgt caagccagac cgctggatcc
     4081 ggcgcaaggc gctggaggac aagatgatcg agccgtttac ggaccggcag gtgcgcgagg
     4141 gagtgatcag ttacggagtg tcgtcgtacg gctacg
//
LOCUS       DASYVO010000267        29778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_120036, whole genome shotgun sequence.
ACCESSION   DASYVO010000267 DASYVO010000000
VERSION     DASYVO010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 29778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29778
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120036"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..3185)
                     /locus_tag="VGR03_08795"
     CDS             complement(60..3185)
                     /locus_tag="VGR03_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1994415.1"
                     /translation="MMLAPGTKLGPYEIVAALGAGGMGEVYRALDMRLDRSVAIKILP
                     AAFSADTDRLNRFEREARSASALNHPNIVTIYELGQYGSTHYIAMELVEGETLRELLV
                     AGLLPIRKAIEIAAQVAEGLAKAHEAGIAHRDLKPENLMVSHDGFVKILDFGLAKLAA
                     PNREQSDICTTSASTSSGLVLGTVGYMSPEQVGGRQLDFRSDQFSFGLVLYEMVTGKR
                     AFQRSTAAETLVAILREQAEPIAVQNRDAPAPLCWAIERCLAKEPEKRYVSTRELARE
                     LAAIRDRFSEKPVKHVEPRPTNIPVQRTGFVGREKEVAAARELLLRPDVRLLTVTGPG
                     GIGKTRLAVEVASGLIDQFPGGIHFVSLSPLSDPGLIASVIVQTLGIGEAGGQSPLEI
                     LKKNFQDSLRAPMLFLLDNFEHLVQAAPTVAELLAMGPNLKILVTSRAALHVYGEREF
                     PVPPLALPDSRSTPSVEVLSQYPAVALFVQRAVAAKPDFELNRENAPAVIEICARLDG
                     LPLAIELAAARIKVLSPSSMRTRLASRLQLLTGGARDLPQRQQTLRAAIDWSYDLLSA
                     AEQKLFRRLSVFVGGCNLEGVEAVCDTKGDLDLDLLDGMASMVDKSLVQQVEQAKGES
                     RFVMLETIREYALEKLETSGEKALTKRAHAAYCLVLAEEEATEQSVAEWVERLAFEHD
                     NFRAGLEWLTETGDAEWGLRLGAALFRFWETREYLAEGRDRLGKLLKLAGAAAPTKLR
                     ARALFSAGALSIEQGDYDSADALIRESLDIARQLRDKQGVAVSLNALAVLARDRGDVT
                     VAHSLFEENLVLWRELGDQKAVARSLSNLANVVKLQGDYARARSLYVECLSIFRGLGD
                     RTGVAWSLNYQGDVARDQGDCAAARTLYEQGLAIFRELGDRWGIAGTLADLGNLASEQ
                     RDSPTARSLYRESLKIFQELEHKRGIARLLECFACSAAAQLEAERSLRLAGAAAALRQ
                     SIGAPLTPAEQAKLESTLDPARQVLTNTAGTTAWLEGWAMPVEKAVEEVLMPHSAARS
                     VAATGPDDPAT"
     gene            3466..4809
                     /locus_tag="VGR03_08800"
     CDS             3466..4809
                     /locus_tag="VGR03_08800"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEV1994416.1"
                     /translation="MNPVKPAPKTGNEEAARVSHDALTVIDSRTGKKYELPITNGTIR
                     ALDLRQIKVNDSDFGMMSYDPAFTNTASCISRIAFIDGDKGILRYRGYPIEELAEKST
                     YLETAYLILHGELPAGKELEEWTYHITHHTFIHESIKKFLDGFHYDAHPMGMLISTVA
                     ALSTFYAEAKDIFNPESRRKQTYRLIGKMPTLAGFAYRHSLGMPYAYPDNDLSYAGNF
                     LNMLFRTTELKYRPNPTLEHALDVLFILHADHEQNCSASAMRNVGSSHVDPYSATAAA
                     AAALYGPLHGGANEEVLRMLLEIGSVSRVPDYIKRVKAGEHKLMGFGHRVYKNYDPRA
                     RIIKRIADEVFEVMGRNSLIDIALECERVALQDDYFISRKLYPNVDFYTGLIYQSMGF
                     PVTMFPVLFAIPRTSGWIAQWQEMLLDREQKIARPRQIYLGHEQRSYVPIGERKQ"
     gene            5401..6576
                     /locus_tag="VGR03_08805"
     CDS             5401..6576
                     /locus_tag="VGR03_08805"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV1994417.1"
                     /translation="MNLALGSLYAWSVFVKPLQGTFKLTREDVSNVFALAVAIFAISF
                     IIAGRLQDKLGPFWVSVTGGLLVSLGFFMCSKATGVSSLFIWFSAIAGIGNGFGYATP
                     IPVMAKWFPDRRGLAVGLAVAGYGGGSAIFAPLSLLYLIPHYGWRTTFEILGVIFLVM
                     TMIGAFLLKNPPVGYKPPGWAPAPASKTAATTYEFKPGEVLRTPAFYFMWIAYALGTS
                     AGLMVISQLAPFAATKGVTAAVGSTLFIIGAAGNAAGRIFSGWMSDALGRLNVLRLMI
                     AIAAIAMVILPHTGGIVTVPVFMYLIVFIAYWCYGTQLSVNASTTSDFWGTKNAGVNY
                     GLLFTAWGVAGIIGPKIGGRMFDIYKDYRIAFYIAAGLAVVALICEFIAKRPKAPTA"
     gene            complement(6731..7744)
                     /locus_tag="VGR03_08810"
     CDS             complement(6731..7744)
                     /locus_tag="VGR03_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEV1994418.1"
                     /translation="MLEVRQVNDHELEAFIGSTQLKGMDSLYIDDVLAVVNRGLDRLP
                     SYLDLYRKAVKQAWNPDELDFSRDRVAWEQLSPETKRRRVWGDRLFFNGEERVASLLA
                     PFVWAAPNKEVEAFAATQLADEVRHTIFFDRYWREVVGTSATSLDELVKEVGIKASEN
                     QAYSYVFNEWLPAQSQWLAAHPRDLDATVRFVTSYHLVVEGALFLTGMRYQLEGARRW
                     GRTWGYYKGFTATTRDESRHVLFGVTFLRDMVKKDPLRFVPIIQSTIQESLPLIPKVM
                     EPPGGDMTYYGGKHLGTAWPGYTPEGLREEMIDYSKGTISRHLHAVGIQMDLESKPSH
                     LPS"
     gene            7961..9361
                     /locus_tag="VGR03_08815"
     CDS             7961..9361
                     /locus_tag="VGR03_08815"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV1994419.1"
                     /translation="MATPKVAPWPAVPTLKPEEVYAGLSDQAKEYPLYLESILRQEHN
                     FEKPEALSRLRVLDCTTRMMIGHWCSSQLAELGAEVIQVEPPGGDPLRQNTPFGRREY
                     MFRDKESGEEVGGQFLHEMRNKLSITLNLEKEEGREIFKKLAVHADVIIENGPPGQWD
                     SWGIGYRQLREINPRLIYCWVGQRGQWGPLKDKPGMLDPVAQAACGFVHGTGDPKEFG
                     GQPTRSALWMADHVGGTAAAMGILSAVLYRENVSGKGQFIEATSAEAIIRILDYSWAW
                     YSMDGSIRPRYGNWDLAINIYAVNPCKDGYMMVGGGHDRLWYRIWRTVGDEYPAVEED
                     ILGDMHLREVADRLGHDMQVKTYTMLGEWLKDNPRSEAERKLSKQQVASGGVLYVNEV
                     AEYPHFKYRGHVAETESPHYGKILYGTTPFLQHKTPGRLKWLGRPTGYDNQDVYRRLL
                     GFTRDDFARLKQSKTV"
     gene            9375..10748
                     /locus_tag="VGR03_08820"
     CDS             9375..10748
                     /locus_tag="VGR03_08820"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV1994420.1"
                     /translation="MSTQAGTPKPGSKQGFEEFCRETFDFKKAFGKPEALKGYRVLSC
                     TQYILGPSCASYMAELGAEVIKIEAPRRGEAMRHTTPFNEPFLYPLSRWVPERGTGLG
                     FLGANPNEYFISVDFHRPEGQDIVKKLAAKSDVIVENYRPGTFERWGIGYRQLKEINP
                     RLIYCWLGGFGGWGPGRVRASYDILGQSQGGNFGMTGKQEFLGGAPSKHTIWLADYWG
                     GMMGAVQILAALYWRDKISGEGTFTEYSQVHGVTRQLEYALPLYGRHGITRERWGNWD
                     TQLCVHGIIKCGKSSYPKSENPQESEEGYILVSAYEDKDFARLCKAVGDGNLAGKYAK
                     QDVRVKAKSQMEIYPWLEKWAADKTKEQVAAILDKADINNQPVWNSKEVANHPHWLER
                     GAVQWLDDPTYGDLLHQGPAYKMSETPPRLKWALKPVGADNEHILGKLAGLSSEDLRR
                     LEEQECI"
     gene            10770..11144
                     /locus_tag="VGR03_08825"
     CDS             10770..11144
                     /locus_tag="VGR03_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994421.1"
                     /translation="MLLICANCKTKNFLDPYPFWNFKGNTKCAGCGKIWRLETSVGAC
                     VSGPDLVEGKPDLLPGFAEKPDYSPISGPGLTRSAPRARPTAVCRPIPIERNVRGKLV
                     SGHPLKKEDLVGSRPRFIQEGM"
     gene            11174..11806
                     /locus_tag="VGR03_08830"
     CDS             11174..11806
                     /locus_tag="VGR03_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994422.1"
                     /translation="MAHYNANSLCVRCKHLRPALDPNLQRAYLGICLKREWPFTLSIA
                     LQGWTECDVFEEAGKAYVPPDPAAAKTAAAAAGKKGPKRLEFYYSSTHKSGETFPCDN
                     ARALELIKKLQAKGVDCKAVDVAGVKDRFPIYHKSVSGPDASVRPVFGAKGALVEDFG
                     SNVPALLVFEGDRYPTLVFPRSDATRGTVRVEQALDDLLAETGQPATASR"
     gene            11819..13468
                     /locus_tag="VGR03_08835"
     CDS             11819..13468
                     /locus_tag="VGR03_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEV1994423.1"
                     /translation="MTDYLPPRELWPTRIYQLPEFATYPARFNPTEQLLDNAVAAGRG
                     ERIALLFEDQRITYAQLLGQANKFGNALRELGIGEGDRVLLRSPTIPPAIVANFAVLK
                     LGAVITPISPLFSRAEIAHVANDAEAVAIIIHAALLGELEAARENLKTVRHIIVIGGE
                     PAELKAKGYRLYGELLQSGGATLAPVRRAREDVAVLLYTSGTTGRPKGTAHLVEETLI
                     VPDTFGKYGWRVTENDVIGGSAPLAFGAGYSTFASIPFRFGAAASLIAKFEPEKMFET
                     IQKHRVTVLSLAPTAYRKMLQLPGAEEKFDLSSLRVCTGGGESLTAPTYFAWKEKFGI
                     DIYEGLGTTEMMYVFASNVVKMHARPGSFGTAVPGFEIKVVDEEGKEAPPREIGHFLV
                     RGPTGTLYWRDPEKQRHAITADGWNRAGDYVYRDEEGYFWFVSREDDVIKSSAYRIGP
                     EEIESTLATHPAVADAGVIGVPDEVRGQIAKAFVVLKPGHTATPEELIEHCRDKIARY
                     KLPREVVFVKELPRTAVGKLLRRVLRQTEAQKAQSAPSGSA"
     gene            13776..14234
                     /locus_tag="VGR03_08840"
     CDS             13776..14234
                     /locus_tag="VGR03_08840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV1994424.1"
                     /translation="MAWTYSHEWLCQRVVEDSQMAIIFADRDGVIHFWNSGAEAMFGY
                     PAGEAMGQTLDLIVPERQRSRHWEGYHKVMATGVTKYGRELLAVPALTKDGRRISIEF
                     SIVLLRAPAGEVLGAAAIMQDVTARWQQQKDLRGRLSALEAKLPQVDKSA"
     gene            14262..14936
                     /locus_tag="VGR03_08845"
     CDS             14262..14936
                     /locus_tag="VGR03_08845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEV1994425.1"
                     /translation="MAKKKIKALVFDAYGTLFDVHSVISACDQSFPGHGAALSQAWRA
                     KQLEYTWLRSLMGRYKDFWQVTESALVFACKSLGLSCPPETRAKLMETYLHLNPFPEV
                     QQALRALSGYSLAILSNGSPRMLKAAVESAGLKGVFSRILSVDDAKIYKPSPRVYRLA
                     PQKLRLATSAIGFISANFWDIAGAKAFGFHTYWVNRSKAPAEELGLVPDATLNSLADL
                     VGVLKV"
     gene            14942..15862
                     /locus_tag="VGR03_08850"
     CDS             14942..15862
                     /locus_tag="VGR03_08850"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV1994426.1"
                     /translation="MRRVLKLVTSFAGLLLLSGAVWTALSSSQSPAPASPCSVEQVAL
                     RTADGVTLSGVVYHPAATPRPFAILLVHGFGGNFYNEYFPLFARTAAEQGYATLALNM
                     RDHDTGPKVSDFIDNEADIAAGLAYLRKQGHSKLILLGQSMGTNRVLYYQAATSDPSV
                     AATVLVAGPGNLFEWNVWQFGRKKAQAIVDEALALQTAGQEHQVMLVDLGPLGKSLYT
                     ARYLLSLRGPNARSDPYRNIQKVTNPILIVQGTADKLVDPGTAARLKSAATSSPRADL
                     ISIEGATHSFGNHQDILARSILTWLKDIAR"
     gene            15971..16702
                     /locus_tag="VGR03_08855"
     CDS             15971..16702
                     /locus_tag="VGR03_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV1994427.1"
                     /translation="MNFQRVTDLLAPDGHVIPAFLFSPDSPRGGAILCPGYGGTKEHM
                     LALAAAIAEKGMAALSIDLCGHGENMTAIGPVMRDELEAGVRYLRRFGRVAAVGISLG
                     GMLALMSSADCMAAISPSVAAKISPQGKWMFENFPSPTVREPYSGYVLELLDALGPVP
                     THNRPCLLLYAERDIPALLDGAAGLKAILPGGELRYVTTDLRPDVQHENGLIRYLPRW
                     FNHSDLKFNAEMLRTVSNWLVGHAT"
     gene            16750..16989
                     /locus_tag="VGR03_08860"
     CDS             16750..16989
                     /locus_tag="VGR03_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994428.1"
                     /translation="MADRSDTMLWEYFDRMVVYTETPEGMVSGLDEWINGAGGDGWEL
                     VSSTPLIAPNKDGVAYGTIGVHLIFKRPHAGAPEG"
     gene            16997..17344
                     /locus_tag="VGR03_08865"
     CDS             16997..17344
                     /locus_tag="VGR03_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994429.1"
                     /translation="MAELRGRALIESRYGPLWSGQQVLTVDGADYDIPALLFHMGLNF
                     EDARAIDVLEVSAGRFVLRYYDGEDQRIVAHEFDANFRFLGEIRGHVAEWIGEDDYFE
                     ASRFLRVRCPADL"
     gene            17470..17793
                     /locus_tag="VGR03_08870"
     CDS             17470..17793
                     /locus_tag="VGR03_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994430.1"
                     /translation="MSTLRGRALIEDRYGPLGRGTQSLTIADVEYALTELMSQMGLGF
                     DDSRAIDALTLSEGRYAVRYFDPQDQRVVVQEFNARFHLLSETRAHSAEWLGEEAYFS
                     LFSGH"
     gene            17831..18682
                     /locus_tag="VGR03_08875"
     CDS             17831..18682
                     /locus_tag="VGR03_08875"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV1994431.1"
                     /translation="MRIIDIHVHIQPFNKLKPAARQVFGLSLREFDQVEKFSADPSAF
                     LKYLDESGVERAGLVTSVSPEVTGYTPEVNDWNARYCAAAPGRLLAFGSVHPLCVSDA
                     GAEVDRLVKIGIRALKVHPPHQLFPPNAYRHGLGPLAAVYERAQAVGLPLMIHTGTSI
                     FPGARNIYARPMLADDLGVDYPDLVIILAHGGRPLWMEEAFFLIRRHRNMYMDVSSIP
                     PQKLLEYFPRLEEVADKVLFGTDWPGLGVRSIRGNIEKFLALPLSEAARRKILYDNAA
                     RLFPSSG"
     gene            complement(18723..19640)
                     /locus_tag="VGR03_08880"
     CDS             complement(18723..19640)
                     /locus_tag="VGR03_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1994432.1"
                     /translation="MEKIAPNTTLIDVEYLGNPRIIAACLVEGQGSVALIDPGPGSAL
                     ETLRRKLDQSGLGVKGIDTILLTHIHLDHAGATGTLVRENPRIRVYVHERGAAHMIDP
                     AKLLASARRLYGDAMERLWGEFLPVPAENVSALAGGETLNIGGRQLEVVYTPGHASHH
                     VSYFDAAEGLAFVGDTAGIRIANSPTILPITPPPDIDPEAWAKSWELIQQRRPERLFL
                     THFGAADHIAEHLEELRERLEEWSIAVRDSLQDGQDDAQRAAQFAQQVATKLKRHVPE
                     EEANRYARGAAPELCWYGLARYWRKRSAN"
     gene            complement(19706..21106)
                     /locus_tag="VGR03_08885"
     CDS             complement(19706..21106)
                     /locus_tag="VGR03_08885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1994433.1"
                     /translation="MSDRQSSPLASAGPAGKESYPRPAYAWYAVGVLTIAYMFSFIDR
                     QILNLLVGPIRRDLGISDTKMSLLMGFSFAVFYSFFGIPLGRLADSKSRRTIIAAGMA
                     LWSLLTAGCGLAKTFAHFLLLRMGVGVGEAALSPSAYSLISDYFPKEKRATAISVYSM
                     AFYIGSGIAFLLGGLVVGFASAKSSWSLPVVGSVRPWQLIFFIVGLPGAVLALLLYTV
                     KEPTRKGARVTKAADGRATVAQVPLRDVIAYMRENGATFLCHNVGFALLAFSSFGSSA
                     WIPTFFIRRYGWTGSHVGVVYGTILCIFGTLGIVAGGRVADWLAQRGYQDASMRVGVI
                     AALAWAPTGILFPLMPSGNLAASLLVPTVFLMGLPVGVAAAAIQEMMPNAMRAQASAF
                     YLFVINLIGLGLGPTAVAMTTDFVFHNDKAIHYSLVVVGILAHLGAATLLWIGLKPFL
                     RSLERSRDWTKLNATG"
     gene            complement(21262..21465)
                     /locus_tag="VGR03_08890"
     CDS             complement(21262..21465)
                     /locus_tag="VGR03_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994434.1"
                     /translation="MNEAQQSIRYSIVVSGVQLLALGLLAEMQVRYYYESRSRSATND
                     GVSILRAESQPPANSPGQPSATP"
     gene            complement(21541..23436)
                     /locus_tag="VGR03_08895"
     CDS             complement(21541..23436)
                     /locus_tag="VGR03_08895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV1994435.1"
                     /translation="MAALLLGGAAKTPTTKESQSRTAGTRGGSGAAGALPERADLFRQ
                     ERVLSLMLIAAGALFLRVHNPFYTTAYMDESIYVVYGRMFLSRHFEAPLDTPLQWSFG
                     WYLWPAMAAIADRIGGLAAVREMAAGLGMVTIAAVYGFARRLFSSAVAVGVAAVMAVL
                     APAVLVSRIATRDSGSICFLALGLWAFSGGWQENRKRDWAAAAACFFAAFLCKYLVAI
                     YFPFLVILALWKRTKSSLFFALPLSAGCAFYAGFHFGDLQHLLRYGAVYGSLRASAGQ
                     AWNIYMGGRWDFWLLVLAAVPTLFVRKWRAAAALLWTGAAVILLFQWNSRADYDYWKH
                     VNYALIFLVPLAVVGVLSVIERFRKTDYGRMLWGVCAVMVLAGAVGWLGKIQSINEFV
                     FWPNVNPILAYFENRLTSNDRVLVDDTVMRYYFQSRLHQYQITDPMYFHYGEYLGEPA
                     YAAAVQDGAFTYVVLDGGMGEEARRMNAAIHPLPSSYHLELAALDPTLGQKIEIYAKP
                     SQTNGTERLPSVRLLAPASNAIVSSKAGATTADGVATGTQPGWHAQIEVFTDRWYLQG
                     GYIPIATDGSFHQTIYLSGEGRQQCYHLVRVRLYNQDGQPLAFSLNYGITRANPDGSV
                     PACRANP"
     gene            complement(23418..25394)
                     /locus_tag="VGR03_08900"
     CDS             complement(23418..25394)
                     /locus_tag="VGR03_08900"
                     /inference="COORDINATES: protein motif:HMM:NF017257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1994436.1"
                     /translation="MKTLGLTTLLCLLLLGGAVNASAQGTATLDRAAAAAAAGRTNEA
                     IQMYRALLEQDPQNAAALSSLSDLLEADGKWREAMPLLERLVKLEPQNSTALYRLGRM
                     KSWQPGGHDEALELLRRACKTSKGDVEICGAYAEILSWGQETRAEAVTNLEAIVSANP
                     DASSVRIRLARILSWNDATRARALALFDNGLQRDPKNIELLLVSAEVLSWRSASRGEA
                     LARCERVLKLDPDNLRALNGKAQLIAWQNRTGEALELYRRVLAKDPNNSAALRGEAEI
                     LNWKGRFVEARTLAQQAHTGAPSDTRASLELARANIGLQRFAQARQAIADVSGNPTPD
                     FADVRQQIQRGLGTYVEFGYAFRQEPANNLEFHRFSAAVSTTLGPSNRVTFLYQPTLF
                     DNHLQGTNSNYFGASLDSTLSDRVTTHEQAGAEVFRNVPVNVDGGVGLSFRPISSTTL
                     KFNFQRQPVEESLLSTNGQNFGGVFFGQVYSNLGDVGISYNNAAHKYDFSLDYTDGVY
                     TGRNLASNRRFSVDSQLGKSLRGDQPYVRVAYYVNYSSFNHDADVQPGQPLSRSTGGY
                     FSPTRYLLNEGILTVGHRFSKTVQWGMSGAAGVQNVENNGSSFSNAQFASSFETHLFW
                     RATPMNELTFSYAYLNVFNAFQRNLFRFAWRHYF"
     gene            complement(25391..27319)
                     /locus_tag="VGR03_08905"
     CDS             complement(25391..27319)
                     /locus_tag="VGR03_08905"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1994437.1"
                     /translation="MVEGKQAAGSQPLPVEKLPPSRQLKLAVTRIGVVLATVFTVRYF
                     YWRILHTMNPAAKIFFYAFLVAEMLSFFESFLFYFITWNPTHRARPEPLPGRSVDVFI
                     PTYNEPLELLRETVMCAINMRYPHKTYILDDGRREEVRRLAQEFACGYFRRADHKDAK
                     SGNLNNALRQTSGEFIVTLDADHVASPELIENTIGFFADPRVGVVQGTQDFYNLDSFQ
                     HRVNWRTRAGWQQQELFFSVIQPGKDRHNATFYCGSPGVVRRKSLEDIGGFPTQSITE
                     DMHTSMRLQKKGWRVLYFNSTLARGLAPQTFSGFARQWQRWGQGSMQVLRAENPFFSK
                     GLSVRQKICYFASLYFYWMSYQKLFFVITPIISLYLGVFVMVTDPLSYASYFLPYFGL
                     NLACSILLQGGWRSFFLSEQFNLLKMHVLMKTISGLFRKDGFFVTPKGRGSAARAPEV
                     LLPLILLAGLATSLVVGVIRIQHTPLYGYFFWALAVNIFWALVFLFMMGGVIGYSMQR
                     KELRESYRFPARLDLRMRVTYPDKNGTLVRQEQFARNLNRFGVSLTMESAIVCGTPIT
                     VELKLPGHTVQASGKVVRNRTYAVNGHSRMSSGIRFDQITAADQDKISKFLFWQIAPR
                     EGQVLRLTHASQTEVERS"
     gene            complement(27805..28383)
                     /locus_tag="VGR03_08910"
     CDS             complement(27805..28383)
                     /locus_tag="VGR03_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994438.1"
                     /translation="MRTASATIVFLLCSAGFSGQRPSSEHRLADSLQRKLDRIRQNAD
                     SARPDQVPTIMTEEEINDYIAAGRVKLPQGVKKVTLQGQSGVITGLATIDFDEIRAGR
                     RTSNPLLSIFSGIHFVRVEADAAGSGGKGKVQVRTVNIDGLDVPRMALEFFINKYLKP
                     KYPTVGLDSEFQMPDRIKEATVGYHKLTVTQR"
     gene            complement(28602..>29778)
                     /locus_tag="VGR03_08915"
     CDS             complement(28602..>29778)
                     /locus_tag="VGR03_08915"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1994439.1"
                     /translation="SRTLSLYATQCVTMSIVDARTPIGQKYAGGDKLPVAVLAQADGK
                     VIGKAANKDGFLRVDQVEKLVETEVKQRDEGLKEKMREAREKAKSGDKDGAISLYRSV
                     YDQKCMFPGKAKDAAKELKKLGLNDVAEVPDAPILDRKLSAEIERVMRNGLKAENAAK
                     YSDAERLYTEAHSMDPADPAPLRYLGELYRHHIGDWDKARTTFEAILKMPADPMSRAV
                     ALHGLGKMTIHEGEFKKGLALMEASVKEYPLALAYRNLAVYWNSEGDVAKADHYTREA
                     LALDPADPYNLVFAAAFLAGNGHAEEALKIARENEAFLPASYNLAAIYAQAGQKEKAL
                     ALLKRHFFEYERYQAVRSKEMMEARVDAVFVSLSKDPAFISLTSGADGKLDPSRAMN"
BASE COUNT         5937 a         8941 c         8944 g         5956 t
ORIGIN      
        1 cggaatgagc gtgggttgga atgcgccggg cgctggtggc ctaagccgtt ggacaccaat
       61 catgtggctg ggtcgtctgg gcccgtggcc gcaaccgagc gagccgcaga atgtggcatc
      121 agcacctcct caacagcttt ctcgaccggc atagcccaac cttctaacca ggctgtcgtg
      181 cctgccgtgt tggtcaatac ctgccgtgcc gggtccaggg tggattccag cttggcctgt
      241 tctgcgggcg tgagcggagc accaatgctt tggcgcagcg ccgccgcggc acctgccagc
      301 cgcagcgaac gctctgcttc gagttgtgcc gccgctgagc atgcgaaaca ctccagcagg
      361 cgggcaattc cgcgtttatg ttccagttcc tggaaaattt ttaggctttc ccggtacaga
      421 gagcgtgctg tgggtgaatc ccgctgttcg ctcgccaagt ttcccagatc agccagtgtg
      481 ccagcaatgc cccagcggtc accgagttcc cgaaaaattg ccaggccctg ttcgtacaat
      541 gtccgcgcgg ctgcgcagtc accctgatcg cgcgcgacat ctccctggta gtttagcgac
      601 caggctacgc ctgtccgatc tcccagtcct cggaagatag agagacactc cacgtaaagg
      661 gagcgcgcgc gggcataatc accctgcaat ttgacgacat tcgccaggtt actgagagaa
      721 cgagcgacag ccttctggtc gcccaattct ctccacagca cgaggttttc ttcgaacagg
      781 gagtgcgcaa cggtgacgtc accccggtcc cgagcaagga ccgccagcgc gtttagagag
      841 acggcgacgc cctgcttgtc acgcaactga cgcgcgatgt ccagactttc cctgatgagc
      901 gcatccgcgg agtcgtaatc tccctgctca atggagagcg cgcctgcgga aaaaagcgcg
      961 cgcgctcgca acttcgtggg agctgccgct cctgcgagct tcagcagctt gcccagcctg
     1021 tccctgcctt cggcgagata ctcgcgcgtc tcccagaagc gaaacagggc cgcgccgaga
     1081 cgcaggcccc actccgcatc tccggtttct gtcagccact caaggcccgc gcgaaaattg
     1141 tcatgttcga acgccaggcg ttccacccat tccgccacgc tctgctctgt agcctcttct
     1201 tctgccagca ccaggcagta ggcggcatgg gcgcgcttgg tcaaggcctt ctccccgctg
     1261 gtctccagtt tttccagagc atactcgcga atcgtctcca gcatcacgaa ccgcgattca
     1321 cccttcgctt gttcgacctg ctgcaccagg cttttgtcca ccatggatgc cataccgtcg
     1381 agcaggtcca aatccagatc gcctttggtg tcgcacacag cttccacgcc ttccagattg
     1441 catcccccca cgaacaccga taatcttcga aaaagcttct gctcggcagc gctcagcaga
     1501 tcgtagctcc aatcaatagc agcgcgaagc gtctgctgcc gctgcggcag gtcccgtgcg
     1561 ccgcccgtca acaactgcaa ccggctcgcc agtcgtgtcc gcatggaaga gggcgaaaga
     1621 actttgatcc gggcggctgc gagttcgatc gctagaggca gaccatctaa ccgggcgcaa
     1681 atctcgatga cggcgggcgc attttcccgg ttcagttcaa aatccggctt ggcagcgacg
     1741 gcacgttgta cgaacagcgc gaccgcgggg tactgtgaca ggacctccac agaaggcgtg
     1801 gaccgcgaat ccggcagcgc aagcggcggg accggaaatt cacgttctcc atacacgtgc
     1861 agcgcggcgc ggctggttac caggatttta agattggggc ccatggccag cagttcggcc
     1921 acggtaggcg ccgcctgaac caggtgttcg aaattgtcca gtaggaacag catgggtgcg
     1981 cgcaatgaat cctggaaatt ctttttgagt atttcgagcg gcgactgacc tcccgcctcc
     2041 ccgataccca acgtctgaac gatcacggaa gcgatcaagc ccggatcgct tagcggggag
     2101 agcgagacaa aatggattcc ccctggaaac tggtcgataa ggccgctggc cacttccacc
     2161 gcgagacgcg ttttgccgat cccgcctgga cccgtgaccg tgagcaagcg cacatccgga
     2221 cgcagcagga gttccctggc ggcggctact tccttctcgc gccccacaaa cccagtccgc
     2281 tgcacaggaa tattggtcgg gcggggctcc acatgcttaa ctggcttttc cgagaagcgg
     2341 tcgcgtatgg ctgcaagttc gcgtgcgagc tctcgggtgg agacgtagcg cttctcgggc
     2401 tccttcgcca gacacctctc gatagcccaa cacagcggag cgggggcgtc gcggttttgc
     2461 actgcaatag gttcggcttg ttcccggaga atggccacca gagtctctgc ggctgtgctt
     2521 ctctgaaagg cgcgctttcc ggtcaccatc tcgtagagca ccaagccgaa cgagaattgg
     2581 tctgaccgga aatccagctg ccggccgccg acctgctccg gtgacatgta tcccaccgtt
     2641 cccagtacca gacccgatga agtcgacgcg gacgtagtgc atatatccga ctgctctctg
     2701 ttgggcgccg caagcttcgc caggccaaag tcgaggattt tcacgaatcc atcgtgagag
     2761 accatcaggt tctcgggctt taagtccctg tgcgcgatgc ccgcttcatg cgccttcgcc
     2821 agcccctccg caacctgcgc cgcgatttca attgcttttc gtattggcag caagcccgcg
     2881 acaagcaact cgcgcagcgt ctctccctcc accagttcca tcgcaatgta gtgagtggaa
     2941 ccgtactgtc cgagttcgta gatggtaacg atattggggt ggttgagggc agatgccgag
     3001 cgcgcttccc gctcaaagcg gttcaaccga tcagtgtctg cggagaatgc ggcgggcaga
     3061 atcttgatcg cgacactgcg atccaggcgc atatccagcg cccggtacac ctcgcccatc
     3121 ccgcccgcgc caagcgcggc gacgatctca taaggcccaa gtttcgtgcc cggagccagc
     3181 atcatggtgg gcaagattat aaccttgagg gaaccatggc acggctcctt ccgctctctc
     3241 ctggacgtat aacaaaatca ccttagatgt gacaacgcgg atttcggcgc tcgtcggtgc
     3301 gtgccgggcc cgacgattct gggggtagtg tcgcctgctc gcaagttccc atcgaggctc
     3361 gcaattccga aacgggtctt tgggaagtca tatgaatacc cctcatcttc ccggcgaact
     3421 tagtgtacat tgacgcagcg tcagcccgcc tgggaggatc accaaatgaa tcctgtgaaa
     3481 ccagcgccta aaaccgggaa cgaagaggcc gcacgcgtga gccatgacgc gctcaccgtc
     3541 atcgacagcc gaacggggaa aaagtacgag ctccccatca cgaacggcac gattcgggct
     3601 ctcgacttgc gccagatcaa ggttaatgac agcgatttcg gaatgatgag ttatgacccg
     3661 gccttcacca acaccgcttc ctgcatcagc cgcattgctt ttattgatgg tgacaagggc
     3721 attctgcgct accgcggcta cccgattgag gagctcgcgg agaagagcac ctatctggag
     3781 acagcttatc tgatcctgca tggcgagttg cccgctggaa aagaactaga agagtggacc
     3841 taccacatca ctcatcacac tttcatccat gaaagcatca agaagtttct ggacggcttc
     3901 cattacgacg cgcatccgat gggcatgctg atttccacgg ttgccgcgct ttcgaccttc
     3961 tacgcggagg ccaaggatat cttcaatccg gaatcgcgcc gcaagcagac ctaccgcctg
     4021 atcggcaaga tgcccacact cgcggggttc gcttaccggc acagccttgg gatgccgtac
     4081 gcctatccgg acaacgacct cagctacgcg ggcaacttcc tgaacatgct ctttcgcacc
     4141 acggagctga agtaccggcc gaacccaact ctggaacacg cactggatgt acttttcatc
     4201 ctgcatgccg atcacgagca gaactgttcg gccagcgcca tgcgcaatgt cggcagctcg
     4261 cacgtggacc cgtactccgc caccgcggct gccgctgccg cgctctatgg cccgctgcac
     4321 ggcggcgcca acgaggaagt gctgcgcatg ctgttggaga tcggctcggt atcgcgcgtg
     4381 ccggattaca tcaagcgcgt gaaagccggc gagcacaagc tgatgggttt cgggcatcgc
     4441 gtctacaaga attacgaccc tcgggcccgc atcatcaagc gcatcgccga tgaagtcttc
     4501 gaggtgatgg ggcgcaactc gctgattgac attgctctcg agtgcgagcg cgtagccctg
     4561 caagacgatt acttcatcag ccgcaagctg tatcccaacg tggacttcta taccgggctg
     4621 atttaccagt ccatggggtt tccggtcacg atgttcccgg tgttgttcgc cattccgcgc
     4681 acgtcggggt ggatcgcgca gtggcaagag atgctgctcg accgcgaaca gaaaattgcc
     4741 cggccacggc agatctatct cggccacgag caacgcagct atgtgcccat cggcgagcga
     4801 aagcagtgat tcgatcgcca gcgtggagat tcgacgagat gagaaccgga gccgcaggtc
     4861 agattgttcg tgctcgttgt gtggctagcg cttcatgtcg ggctgccatc tttgcggata
     4921 tgcgtggggc ccatccgcac cacagctatt cgtagcgacg ccggctaggc ggggaaacgt
     4981 tggcggcgtc gcccagtcca agattctgaa caaatttgcc ttgacaatcc gaaatcgaga
     5041 ggacgatact ccgcggcgag acatgactcg gcaggcccca tcataaagat tccacgaacc
     5101 ccacgttggc gatgtatgtg tggtacccac ggcgtaacgg gggggaggca ctcagatgca
     5161 gaatcagcgg caccccttgc ttcgggggga ggtgtcggcc atcctacgcc accgagcaag
     5221 aggccggttt gcgccagttc ttgaacactt agcattcact gcagttgagt tcgtaacagg
     5281 acttggtgcg cctgaagctt tctatcggtc tagagatggg ttgcgattta tcctgacggg
     5341 aggagaatga tgccaaacgg agagattagt cgttggtggc gagtagtcgg cggcatgtca
     5401 atgaatcttg ccttgggctc tctttatgcc tggagcgtat tcgtaaagcc gctgcaaggt
     5461 accttcaagc ttacgcgcga ggatgtgtcc aacgtgttcg cgttagcggt ggcgatcttt
     5521 gcgatctcgt ttatcattgc cgggcggctc caggataaac tggggccttt ctgggtctca
     5581 gtcaccgggg gcctgctggt gagcctcggg ttcttcatgt gctccaaggc gaccggtgtg
     5641 tcgtcactat tcatttggtt tagcgcgatc gccggtatcg gcaacggctt cggctacgcc
     5701 acgccgattc ccgtgatggc caaatggttc cctgatcgac gaggtttggc ggtcgggctc
     5761 gccgtggcag ggtacggagg tggctcagcc attttcgcgc cgctgtccct tctctacctg
     5821 atcccgcact atggctggcg gaccaccttc gagattctgg gagtcatatt cctggtcatg
     5881 accatgatcg gagcatttct gttgaagaac ccgccggtag gttacaagcc tcccggatgg
     5941 gccccggccc cggcctccaa gactgcggca accacctatg aattcaagcc gggtgaagtg
     6001 ctgcgcacgc ctgcgttcta tttcatgtgg attgcctatg ccctcggcac ctcggcaggt
     6061 ttgatggtca tcagccagtt ggccccgttc gccgccacca agggagtcac cgcagccgtt
     6121 ggatccacac ttttcatcat cggggctgcc ggcaacgccg ccggccggat cttttctggc
     6181 tggatgtccg acgccctcgg ccgactcaac gtcttgcggt tgatgatcgc gatcgcagcc
     6241 attgccatgg tgatcttgcc ccacaccggc ggcattgtca ctgtccccgt gttcatgtac
     6301 ttgatcgtgt tcatcgctta ttggtgctat ggcacccagc tctcggtgaa tgcctcgacc
     6361 acgtctgatt tctggggaac gaagaacgcg ggcgtcaact atgggctgct gtttacggct
     6421 tggggcgtgg ctggaatcat cggaccgaaa atcggcggcc ggatgttcga catatataag
     6481 gactatcgaa ttgccttcta catcgcggcg ggcctggcgg tggtcgcgct gatttgcgaa
     6541 ttcattgcga agcggcccaa agccccgacc gcataagaag ctcaaaggac cgcaggttcg
     6601 aagaggtcta agggaagatc gaagcgcgca gaaaacggat tctcacggga cgagtcaccc
     6661 ggcgagcttc catcggtttt gtgcagagga aggcttaagt cccccggcgt tggcggggac
     6721 tcgcgcgctc ttagcttggc aggtgcgacg gcttactttc gagatccatc tggatgccca
     6781 cggcgtgcag gtgccgtgag atagtgccct tcgagtagtc gatcatttcc tcgcgcagcc
     6841 cctcgggcgt gtacccgggc caagcagtgc ccagatgctt gccgccatag taggtcatgt
     6901 ccccaccagg gggttccatg accttcggta tcagaggaag cgattcctgg atcgttgact
     6961 gaatgatggg cacaaaacga agcgggtctt tcttcaccat gtctcgcagg aacgtgactc
     7021 caaacaggac atgccgcgat tcgtcccgcg ttgtggcggt gaagcccttg taatagcccc
     7081 atgttcttcc ccaacgcctg gcaccttcga gctggtagcg cataccagtg aggaacaacg
     7141 cgccctcaac caccaagtgg taggaagtta caaatctcac ggtggcgtcc aggtcccggg
     7201 gatgtgcggc caaccactgc gactgcgcgg gcagccattc attgaaaacg tagctgtatg
     7261 cttggttttc gctcgccttt attcctactt ccttcaccaa ctcgtccaag ctggtagccg
     7321 acgtacccac aacctcgcgc caatagcgat cgaagaatat ggtgtgccgg acctcatccg
     7381 ctaactgtgt tgcagcaaag gcttcaactt ccttgttcgg tgcggcccag acgaacggcg
     7441 ccagcaacga agccacgcgt tcctccccgt tgaagaagag tcgatcgccc cagacgcgcc
     7501 gcctcttggt ttccggcgat agttgctccc atgccactct gtcgcgcgaa aagtcgagtt
     7561 cgtcgggatt ccaggcctgc ttcaccgctt tgcgatacag atccagatac gaggggagcc
     7621 tgtcgagacc gcggttgacg acggccaaga cgtcatcaat gtacagactg tccatcccct
     7681 taagctgggt gctgccgatg aatgcctcga gttcgtgatc attgacctgt ctcacttcga
     7741 gcatcgctac acctctccct tgggctatga ataccggcga aacttaccac gcgcgaagag
     7801 aagcgtcaag gcggccgggg aatggaacgc ggcctgaaat cacttgacat tcgtcctccg
     7861 caggcatata cgcacatcat ctgccccact cagctcccgg gtcagaccca ccaatagaga
     7921 acagtaatga gcaagtccga cattcgcagg aggcaatgaa atggccactc caaaagttgc
     7981 gccttggcct gcggttccca cgcttaagcc ggaagaggtg tatgccggtc tgagcgacca
     8041 agccaaagag tacccgctgt acctggaatc catcctgcgc caggagcaca atttcgaaaa
     8101 gccggaggca ctgtctcgtc tgcgcgtgct cgattgcacg acgcgcatga tgatcgggca
     8161 ctggtgctcg tctcaattag ccgaactggg cgccgaggtc atccaggtgg aaccgccggg
     8221 aggagacccc ctgcggcaga acaccccatt cggcagacgg gagtacatgt tccgcgacaa
     8281 ggaaagcggc gaggaagtcg gcgggcagtt cttgcacgag atgcgcaaca agctttcgat
     8341 taccctgaac ctggagaaag aggaaggccg cgagattttc aagaagctgg ctgtccacgc
     8401 cgacgtgatc atcgaaaatg gcccgccagg gcagtgggac agttggggca tcggctatcg
     8461 tcaactgcgg gaaatcaatc cgcggctgat ttactgctgg gtcggccagc ggggccagtg
     8521 gggcccgctg aaggacaagc cggggatgct cgacccggtg gcccaggctg cctgcggatt
     8581 tgtccacggc accggcgacc cgaaagagtt tggcgggcag cctacccgct ccgccttgtg
     8641 gatggccgat cacgtgggcg ggacagcggc ggccatgggg attctttccg cggtgttgta
     8701 ccgggaaaat gtttccggca agggtcagtt catcgaagct acgtccgccg aggctatcat
     8761 ccgcattctg gactacagct gggcctggta ctcgatggac ggcagtatcc gcccgcgcta
     8821 cggtaactgg gatctggcga tcaacatcta cgccgtcaat ccttgcaagg acggctacat
     8881 gatggtcggc ggcggccacg accgcctgtg gtaccgcatc tggcgcacgg tgggtgacga
     8941 gtaccctgcg gtcgaagagg acatcctcgg cgatatgcac ctgcgcgagg tcgcggaccg
     9001 gctggggcat gacatgcagg tgaagaccta caccatgctg ggagagtggt tgaaagacaa
     9061 tccgcgcagt gaggccgagc gcaaactttc caagcagcag gtggcctccg gcggcgtgct
     9121 ttacgtgaac gaagtggcgg agtacccgca cttcaaatat cgcggccacg tggccgagac
     9181 cgagtcgccc cactacggca agatcttgta tggcacgacc cccttcttgc agcacaagac
     9241 tccgggtcgt ctcaagtggc tcggccgccc gaccggctac gacaaccagg acgtatatcg
     9301 ccgactgctc ggcttcaccc gcgacgactt cgcgcggcta aagcaatcga agaccgtctg
     9361 accaggagga tgtgatgtct acccaagccg gtacgcccaa gccggggtca aaacagggtt
     9421 ttgaagagtt ctgccgcgag accttcgact tcaagaaggc ttttggcaag ccggaggcgc
     9481 tgaagggtta ccgcgtgctt tcctgcacgc agtacatcct cggtccgtcc tgcgcctcgt
     9541 acatggcgga actcggcgcc gaggtcatca agattgaggc gccccggcgg ggcgaggcca
     9601 tgcgccatac gacgccgttc aatgagccgt ttctgtaccc gctctctcgc tgggtgccgg
     9661 agcgcggcac cggcctggga ttcctcggcg ccaaccccaa cgagtacttc atctcggtgg
     9721 acttccaccg tcccgagggt caggacatcg tgaagaaact ggcggccaaa tccgacgtga
     9781 tcgtggagaa ctaccgcccc ggcactttcg agcgctgggg catcggttac cggcaactga
     9841 aggaaatcaa tccccggctc atatattgct ggctcggggg tttcggcggc tggggcccgg
     9901 ggcgtgtgcg ggcttcctac gacatcctgg gtcaatccca gggcggcaac tttggcatga
     9961 ccggcaagca ggaattcctc gggggcgcgc cctccaaaca caccatctgg ctggcggact
    10021 actggggcgg catgatgggc gcggtgcaga ttctggccgc gctgtactgg cgtgacaaga
    10081 tttccggcga gggcaccttc accgagtatt cgcaggttca cggagtcacg cgccaacttg
    10141 agtatgctct gcccctgtat ggccggcacg gcatcacccg agagcgctgg ggcaactggg
    10201 atacccaatt gtgcgtgcac gggatcatca agtgcggcaa atcctcctac ccgaaatcgg
    10261 agaacccgca ggagtcagag gagggctaca tcctcgtcag cgcctacgag gacaaggact
    10321 ttgctcgcct ctgcaaggct gtcggtgacg gcaacctggc gggaaaatat gcgaagcaag
    10381 acgtccgggt aaaggccaag agtcagatgg agatttaccc ctggctggag aagtgggcgg
    10441 cggataagac gaaggaacag gttgccgcga ttctcgataa ggcggacatc aacaaccagc
    10501 cagtctggaa ctccaaggag gtcgccaatc acccgcactg gctggagcgt ggcgcagtcc
    10561 aatggctgga cgaccccacc tacggtgact tgttgcacca gggcccggcg tacaagatgt
    10621 ccgaaacccc gccgcgtctc aagtgggcgc tcaaaccggt gggcgcggac aacgagcaca
    10681 tcctgggcaa actggctggg ctgagctcgg aagatttgcg gcgactcgaa gagcaggaat
    10741 gcatctgagg ggacagtgag gagataccca tgctgctgat atgcgccaac tgtaagacga
    10801 agaattttct tgacccttat cctttttgga atttcaaggg caacaccaag tgcgccgggt
    10861 gcggcaagat ctggcggctg gagacctcgg ttggagcctg cgtgtctggg cccgatttag
    10921 tagaaggcaa gcccgacctt ttgccgggtt tcgcagagaa acccgactac agccccatct
    10981 ccggcccagg gctgacgcgt tcggcgccac gtgcgcgccc cactgccgtc tgccgcccca
    11041 ttcccataga gcgcaatgtg cgcggcaagc tcgtaagcgg gcacccgctc aagaaagaag
    11101 atctggtggg tagccggccg cggtttatcc aagaaggtat gtagaatttg caattcccag
    11161 attggaggcc catatggccc actacaatgc caattccttg tgcgtccgct gcaagcatct
    11221 gcgtccggcg cttgacccga acctgcagag agcctacctc gggatatgcc tgaagcgcga
    11281 atggcctttt acgctatcga ttgcgctcca gggctggacg gagtgtgacg ttttcgaaga
    11341 ggctgggaag gcgtacgtcc cgcccgatcc ggcagccgcc aagactgcgg ctgcggccgc
    11401 cggcaagaag ggtccaaagc gcttggaatt ctattacagc agcacccaca agtccggtga
    11461 gacatttccc tgcgataatg cgcgcgctct tgaactcatc aagaagttgc aggccaaggg
    11521 cgtggactgc aaggcggtgg atgtggccgg agtcaaagac cgtttcccga tttaccacaa
    11581 gtcggtcagc ggcccggacg cctccgtgcg cccggtgttt ggcgcgaagg gagcgctcgt
    11641 ggaggacttt ggctcaaacg tccccgcgtt gctcgttttc gagggtgacc gctatcccac
    11701 gctggtgttc ccgcgcagcg acgccacgcg cggaacggtt cgagttgagc aggcgttgga
    11761 tgatcttctg gccgagaccg gccagcccgc cacagcgagc cgctgacacg aggctaatat
    11821 gaccgactac ctgccaccgc gtgagctgtg gcccacgcgc atttaccaat tgcctgagtt
    11881 tgctacctac cccgcccgat tcaatcccac ggaacaactg ctcgacaacg ctgttgccgc
    11941 gggcagaggc gagcgcattg cgcttctgtt tgaggaccag cgcattacct acgcgcaact
    12001 cctcggccag gccaacaaat tcggcaacgc gctgcgcgag ctgggcatcg gcgagggcga
    12061 ccgggttctt ctgcgctcgc cgacgatccc gcccgctatc gtcgccaact ttgcagtgct
    12121 caagttgggt gccgtgatca cccctatctc tccactgttc tcgcgtgccg agattgccca
    12181 cgtggccaac gatgccgagg ctgtggccat catcattcac gccgcgctct tgggcgaact
    12241 cgaagccgcg cgcgaaaatc taaagacggt tcggcacatc atcgtcatcg gcggcgaacc
    12301 ggcggaactc aaggccaagg gctaccgact ctacggagaa ctgctgcaat ccggcggtgc
    12361 gacactcgcc ccggtgcgcc gtgcgcgcga agatgtggcc gtgctgctgt acacttccgg
    12421 cacgaccggc cggcccaagg gcacggccca tctcgtcgaa gagacgctca tcgtgcccga
    12481 cacctttggc aagtatggct ggcgggtcac tgagaatgac gtcatcggag gctcggcgcc
    12541 actggccttc ggcgcgggct attcgacttt cgcgagcatt ccgttccgct tcggagcggc
    12601 tgcctcactc attgccaagt tcgaacccga aaagatgttt gagaccatcc agaaacatcg
    12661 cgttaccgtg ctctcgctcg cgcccaccgc ttaccgcaag atgctgcaat tgccgggcgc
    12721 cgaagagaaa ttcgacttga gcagcctgcg cgtatgcacg ggcgggggtg agagcctgac
    12781 ggcgccgacc tatttcgcct ggaaggaaaa gttcggcata gatatctacg agggtttggg
    12841 cacgaccgag atgatgtatg tattcgcctc gaacgtagtc aagatgcacg cccggccggg
    12901 gtcgttcggc actgccgtgc cggggttcga gatcaaggtg gtggacgaag aaggcaagga
    12961 agcgccaccg cgggagatcg gccatttcct ggtccgcggg ccgacgggaa cgctttactg
    13021 gcgcgaccca gagaagcagc ggcatgcgat tactgcggac ggctggaacc gggctggcga
    13081 ctacgtctat cgcgacgaag agggctattt ttggtttgtt tcccgagagg atgacgtcat
    13141 caagagttcg gcgtaccgca tcgggccgga ggagatcgaa agcacgctgg cgacgcatcc
    13201 ggccgtggcg gacgcgggcg tgatcggcgt gcccgacgag gtgcgcggcc agatcgccaa
    13261 ggccttcgtc gtgttgaagc caggtcatac cgctacgcct gaagaactga tcgaacactg
    13321 cagagacaaa atcgccaggt acaagctgcc gcgagaggtg gtcttcgtga aggagttgcc
    13381 acggacggcc gtaggcaaac tcctgcggcg ggtgctgagg cagacagaag cccaaaaggc
    13441 gcagtcggcc ccttcggggt ccgcttgaag cgcacgtaga tgcgcgcgag cctcgttacc
    13501 gttggctgct ctctgagcat ctttccttcc ggtccgcgag tgatctttta gcttttgttc
    13561 atgagacgcg acgtcactcg ccagcgagaa gctgtccggc caggctctcc gagtgctcgc
    13621 gctcgcagat tctccaccct ttcgctcact cgggaagccg acgcagctca cttggagcga
    13681 acggacatac aatcgtaacg acgaaggagt cgggtctccg tgttcaccta gacccggtgt
    13741 ggcgagcatt gggggtcttt tcccaggagg gtgccatggc gtggacctat agtcatgagt
    13801 ggctgtgcca gcgcgttgtg gaagactcgc agatggccat catttttgcg gatcgtgacg
    13861 gcgtcattca tttctggaat tcaggggccg aagcgatgtt tggctaccct gctggggagg
    13921 ccatggggca gaccctggat ctaatcgtcc ccgagcgcca gaggagccgt cactgggagg
    13981 gctaccataa ggttatggcc actggtgtca ccaagtacgg gcgggagttg ctggcggtgc
    14041 cggccttgac gaaggatggt cggcggatct ccattgaatt cagcattgtg ctcctgcgtg
    14101 ctcccgccgg cgaagtgctg ggcgcggcgg ccatcatgca agatgtaaca gcgcggtggc
    14161 agcagcagaa ggatctgagg gggcgcctgt cggccctgga ggccaagttg ccacaggtag
    14221 acaaatccgc ttgagacgtg caaaaaaggt tgctgacaag aatggcgaag aaaaaaatca
    14281 aagcgctcgt gttcgacgcg tacggaacac ttttcgacgt tcactccgtt atttccgcct
    14341 gcgatcagag ctttccgggt catggcgccg ccttgagcca agcctggcgc gctaaacagt
    14401 tggaatacac ctggctccgc agcctgatgg ggcgttacaa agacttctgg caggtgactg
    14461 agtctgcgct cgtttttgcg tgcaagagtc tgggtttgtc gtgcccaccg gagacgcgcg
    14521 ccaagttgat ggagacttat ctccacctga acccttttcc tgaagtccag caagcactga
    14581 gggctttatc cggctattcg ttggcgattc tgtccaacgg ttctccacgg atgctgaaag
    14641 cagcggtgga gagcgccggc ctgaaaggag tgttttcccg catcctgagc gtggacgacg
    14701 cgaaaatcta caagcccagc ccgcgcgttt atcggttggc gccgcagaaa ttgcgtttgg
    14761 ccacaagcgc gatcggcttt atttccgcca atttttggga cattgccggc gcaaaggcct
    14821 ttggctttca cacttactgg gtaaaccgct ccaaagcgcc cgcggaggag ctcggcctgg
    14881 tcccggatgc aacgctcaat tcgctggcag acttggtcgg cgtcttaaag gtctagtctc
    14941 gatgcgacgt gtgctcaagc tagtgacaag cttcgccggt ctcttgttgc tcagtggggc
    15001 ggtgtggacg gctctttctt ccagccaatc accggctccg gcatcaccct gctccgtcga
    15061 gcaggttgcg ctgcgcacgg ctgatggcgt cacgctgagc ggcgtggtct accatccggc
    15121 tgcgactccg cgcccgttcg ccatcctgct tgtgcatggc tttgggggca acttctacaa
    15181 cgaatacttt ccgctgtttg cgcgaacggc cgccgagcaa ggctacgcga ctttggcgtt
    15241 gaacatgcgc gatcacgata ccgggcccaa ggtatccgat ttcattgaca acgaagctga
    15301 cattgccgcc ggcttggcct acctgcgaaa acagggtcat tcgaaattga tactcttggg
    15361 ccaaagcatg ggcacgaacc gagtgttgta ctaccaggct gccacgagcg atccgagcgt
    15421 cgcggcgacc gtgctcgtcg cggggccagg gaatttgttt gagtggaatg tctggcagtt
    15481 tggccgaaaa aaggctcagg ctatcgtcga tgaggcgctg gcgctgcaaa cggccggtca
    15541 agagcaccag gtgatgctgg ttgatctggg gccgctcggc aaatccttgt atactgcgcg
    15601 ctacttgctc agtctgcgcg ggccgaatgc tcgatcggac ccgtaccgga acatccagaa
    15661 agtgaccaac cccatcctga ttgtgcaggg aaccgctgat aaactggttg atccgggtac
    15721 cgccgctcgg ctcaagagcg cggcgacgtc cagtcccaga gcagacctga tttccatcga
    15781 gggagccact cactctttcg gaaaccatca ggacattctg gcgcggagta tactcacttg
    15841 gctcaaagac attgcacgct gataggaatt ggccgacgtc ggggctgtat ctatacgccg
    15901 gctgctccag cttcggtctc agtttttccg gtttagccgg ccgcgatcta agactgtgtc
    15961 ttgaggactc atgaactttc agagagtcac tgacctgctt gccccggatg ggcatgtcat
    16021 cccggctttt cttttctccc ccgattcgcc gcgcggcgga gcgatcctgt gtccaggtta
    16081 tggcgggacg aaggagcaca tgctggctct ggcggccgcg attgcggaaa aggggatggc
    16141 tgctctttcg attgatttgt gcgggcacgg agagaacatg accgcgatcg ggccggtgat
    16201 gcgcgacgaa ctggaagcgg gggtgcgcta tcttcgtcgt tttggccgcg tcgcggccgt
    16261 cggcataagt ctaggtggca tgctcgctct catgtcttcc gcagattgca tggcggcgat
    16321 ctcgccgtcg gtggcggcga aaatctcacc gcagggcaag tggatgttcg agaatttccc
    16381 gagtccgact gtgcgcgagc cctactccgg ctacgtcttg gaattgctcg acgcgctcgg
    16441 tcctgttccc actcacaacc gtccctgcct gctcttgtac gcggaacgcg acatcccggc
    16501 gcttctggac ggtgctgccg gattgaaggc gatcctgccc ggaggggagc tgcgctacgt
    16561 cacgaccgac ttacgtccgg acgtgcagca cgagaacgga ttgattcgct acctgccgcg
    16621 ctggttcaac cacagcgatc tgaaattcaa tgccgaaatg ctcaggacgg tatccaattg
    16681 gctggtcggc cacgctacat gaccgcaggg cctgaacaga tgcgcatctc taactcggta
    16741 tgaggtagga tggcggacag gagcgacacg atgctgtggg agtacttcga tcgcatggtt
    16801 gtctacaccg aaacgccgga gggcatggtg agcgggctgg acgagtggat caatggggcc
    16861 ggcggcgacg gctgggaact ggtgagctca acccctctga ttgcgccgaa taaggacggc
    16921 gtggcctatg gcacaatcgg cgtgcacctg atattcaaga ggcctcacgc gggggcgccg
    16981 gaagggtagg cgggatatgg cggagttgcg gggccgggcg ttgatcgaaa gccgatacgg
    17041 gccgttgtgg agcgggcagc aggttctgac cgttgacggc gcggactacg acataccggc
    17101 gctgctgttc cacatggggc tcaacttcga agatgcccgg gccatcgatg tgctggaagt
    17161 gtctgccggg cgctttgtct tgcgctatta cgacggagaa gaccagcgca tcgtcgccca
    17221 cgagttcgat gccaatttcc gatttctcgg cgagatccgc ggacacgtcg cggagtggat
    17281 cggcgaggat gactacttcg aagcgtcccg gtttctccgc gtgcgctgtc cggctgatct
    17341 gtaagccggg cctccggccg tccttggagc aggcacgctg catcgcgccg gcttggtgtc
    17401 cgccgaagtt ggttgtgtgg caggtttccg cgtggctcgg cagccaggcg ccagaagcgt
    17461 gagcgctcta tgagtacgct gcgagggcgg gccctgattg aggaccgcta tggccccctg
    17521 ggcaggggaa cgcagagcct gacgatcgcc gacgtcgaat atgcgttgac cgagttgatg
    17581 tcccagatgg gactcggctt cgacgatagc cgggcgatcg acgcgctgac actgtccgaa
    17641 ggacgctacg ccgtccgata tttcgacccg caggaccagc gcgtagtcgt ccaggaattc
    17701 aacgcgcgct tccacttgct cagcgagacg cgcgcgcact ccgccgagtg gcttggcgag
    17761 gaagcctatt tttcgctgtt cagcggccat tgaaccggcc cgcctgcgga ctgagccctg
    17821 atctgccgtc atgcgcatca tcgacattca cgtccacatc cagccgttca acaaactcaa
    17881 gccggcggcg cggcaggtgt ttggactcag cctccgcgag ttcgaccagg tcgaaaagtt
    17941 ctcggcggat ccctctgcct ttctgaaata ccttgacgag tcaggcgttg aacgcgccgg
    18001 gttggtcact tctgtcagcc cggaggtaac tggttacacg ccggaagtga acgactggaa
    18061 tgcgcggtac tgcgctgcgg cgcccggccg cttgctcgcc tttgggtccg tccatccgct
    18121 atgcgtgagc gatgccggcg ccgaagtcga tcgcttggtg aagatcggca ttcgcgcgct
    18181 gaaggtgcat ccgccgcatc aacttttccc gccgaacgcc taccgtcacg gcctggggcc
    18241 gctcgcggct gtttacgagc gcgcgcaggc cgtcggcctg cccctgatga tccataccgg
    18301 cacttcgatt tttcccggcg cgcgcaacat ctatgcgcgg cccatgctcg cggacgatct
    18361 gggtgtggac tatccggatt tggtgatcat tctcgcccat ggcggccgcc cgctctggat
    18421 ggaggaggct ttctttctta tccgccggca ccggaacatg tacatggacg tctccagcat
    18481 cccgccgcaa aagctgctgg aatattttcc gcggctggag gaggttgccg ataaggtgct
    18541 cttcggcaca gactggcctg gcctgggcgt gcggagcata cgcgggaaca tcgaaaaatt
    18601 cctggccctg ccgctttcgg aagcggcccg gcgcaagatt ctttatgaca acgccgcgag
    18661 gctgtttccc tcgtccgggt aattcccggc gcaggctatt tcgaagatgg aaggccatag
    18721 gttcaattcg cgctgcgttt gcgccagtat ctggccagcc cgtaccaaca gagctccggc
    18781 gccgcgccgc gcgcatagcg gttggcttcc tcctcgggaa cgtggcgctt caactttgtc
    18841 gcgacttgct gcgcgaactg cgcggcccgt tgtgcatcgt cctggccatc ctggagtgaa
    18901 tcgcgcacgg cgatgctcca ctcttccagc cgctctcgca actcctcgag gtgttcggcg
    18961 atgtgatcgg cggcgccaaa gtgcgtgagg aaaagccgtt ccggccgccg ctgttgaatc
    19021 aactcccagc tttttgccca ggcctccggg tcgatgtcag gcggcggcgt gatgggcagg
    19081 atggtcggtg aattggcgat ccggattccc gcggtgtctc ccacaaacgc cagcccctcc
    19141 gccgcgtcga agtagctcac gtggtgggag gcgtgcccgg gcgtgtaaac cacttccagt
    19201 tggcggccgc cgatgttcag tgtttcgcct cccgccagcg cactgacgtt ttccgccggc
    19261 acgggcagaa actcgcccca gagccgctcc atcgcatctc catagagacg ccgggcgctg
    19321 gcgaggagct tcgccggatc gatcatatgc gcggcgcctc gctcgtgaac gtagacccgg
    19381 atgcgagggt tttcgcgcac cagcgtcccg gtagcgccgg cgtgatcgag gtggatatgc
    19441 gtgagcagaa tcgtgtcgat gcccttgact ccgagacccg attgatcgag cttccttctg
    19501 agtgtttcga gcgccgaacc gggccccgga tcaatcaacg ccacgcttcc ctggccttcg
    19561 acgaggcatg ccgcaatgat ccttggattg ccaaggtact ccacgtcgat gagggtggtg
    19621 ttgggagcga ttttttccat gtggaatccc cagattctat ccgcgcccgg tgcggacggt
    19681 caattgcaga agccgcgccg cccggtcatc ccgtagcgtt gagcttcgtc caatcccttg
    19741 agcgctcgag gctgcgcaaa aaaggcttta ggccgatcca caggagcgtc gctgctccga
    19801 ggtgcgctaa gatccctacg acgaccagcg aatagtggat tgccttgtca ttgtggaaga
    19861 cgaagtccgt cgtcatggct accgcggtcg ggccgaggcc gaggccgatg aggtttatca
    19921 cgaaaaggta gaacgcggat gcctgggcgc gcatcgcgtt gggcatcatt tcctggatcg
    19981 ccgccgccgc gacacccacg ggcagcccca tcaggaaaac cgtaggaacc agtagggagg
    20041 cggccaggtt tccgctgggc atcaagggaa agagtattcc cgttggcgcc cacgccaggg
    20101 ccgcgatcac cccgacgcgc atgctggcgt cctgataacc gcgttgcgcc agccagtccg
    20161 ccacccggcc gccggcgacg atccccagcg tcccgaatat gcaaaggatt gtgccgtaga
    20221 ccacgccgac gtgggagccc gtccagccat accttcggat gaaaaaggtc ggaatccacg
    20281 cggagctgcc gaagcttgaa aaagccagca aggcgaatcc gacattgtgg caaaggaagg
    20341 tcgccccgtt ctcgcgcatg taggcgatga cgtcgcgcaa aggcacttgg gcaaccgttg
    20401 cccttccgtc cgcggctttc gtcacgcggg cgcccttccg ggtgggttcc ttcaccgtgt
    20461 agagaagaag agcgagcacg gcccctggta accccacaat gaagaagatc aactgccacg
    20521 gccggacgga acccaccacc ggcaggctcc aactggattt tgcggaagcg aatccgacga
    20581 ccaacccgcc cagaaggaat gcgatgcctg agccgatgta gaacgccatg gagtacacgc
    20641 tgatggctgt ggcccgcttt tccttgggga agtagtcgga gatgagagag taggccgacg
    20701 gcgagagagc agcttcaccg acgcccactc ccatccgaag cagcaggaag tgcgcgaagg
    20761 tcttggccag cccgcacccg gccgtcaaca ggctccaaag cgccatgccg gcggcgatga
    20821 tggtgcggcg gctcttcgaa tctgcgagcc ggccaagagg gatgccaaag aaagagtaga
    20881 acaccgcgaa gctgaagccc atcagcagac tcatcttcgt gtcactgatg ccgagatccc
    20941 gccggatggg ccccaccagc aaattcagaa tttgccggtc gataaaggaa aacatgtagg
    21001 cgatcgtgag gactcccacc gcgtaccagg cgtaggcagg ccgcggatac gattccttcc
    21061 cggcggggcc tgcactcgct aacggcgaac tctggcggtc ggacaaagcg tcctcttcat
    21121 tcttcgatcc ggctaaggca cttggtgcgc atcggtgagc atgcgcgcga cgcagctatg
    21181 ttaatccgtt cctccagcct gggccactcc ttgaacctgt ctctcgccaa aacacacttg
    21241 tggattggga gtcgtagccg atcacggtgt cgcagatggt tgtccggggg aattggccgg
    21301 gggctggctc tcagcgcgca ggatgctgac cccgtcattg gtcgcacttc ggctgcggct
    21361 ttcgtagtag taacgcacct gcatttcggc cagcagcccc agcgcgagaa gttgcacgcc
    21421 ggatacgacg atggaatatc gaatggattg ctgagcttcg ttcacagaac tcagtttccc
    21481 cggtccgatg gaagctcttg atcgtacaac gaataagccc gtcgccgggg ccgcgcggca
    21541 ctacgggttg gctcggcagg ccgggaccga accatccggg ttggcgcgag tgatgccgta
    21601 gttcaggcta aaggcaagcg gttgaccgtc ttgattgtag agccggaccc tcacaaggtg
    21661 ataacactgt tgccggcctt cacccgacag gtaaatcgtc tggtgaaaac tgccgtccgt
    21721 ggcaatgggg atgtacccgc cttggagata ccagcggtcc gtaaacactt cgatctgcgc
    21781 gtgccagcca ggctgcgtcc ctgtcgcgac gccgtcggcg gtggtggctc cggccttcga
    21841 gctcacaatc gcattgctgg cgggagcaag aaggcgaacg ctcggcagtc tttcggtacc
    21901 atttgtctgc gaaggctttg cgtagatttc tatcttttgg cccagggtcg ggtcgagcgc
    21961 agccagttcc aggtgatagg aacttggcag aggatgaatg gcggcattca tgcgccgcgc
    22021 ctcttctccc atgccgccgt cgagcaccac gtaggtaaac gcgccatcct gcaccgcagc
    22081 cgcgtatgcc ggttctccca gatattcccc gtaatggaaa tacatgggat cagtgatttg
    22141 atactggtgc agcctagatt ggaagtaata gcgcatcacg gtgtcgtcaa ccaggacgcg
    22201 atcgttggat gtgaggcggt tttcgaagta cgccaggatg gggttcacat tgggccagaa
    22261 tacaaattca tttatgctct ggatcttgcc cagccaaccg accgcgccgg ccagaaccat
    22321 tacggcgcag acgccccaca gcatccggcc gtaatcggtt ttcctgaacc gctcaatcac
    22381 ggacaatacc ccgacaaccg caagaggaac caggaaaatc agcgcatagt ttacgtgctt
    22441 ccagtaatcg taatcagccc tcgagttcca ctgaaaaagc aggatgacag cggcgccggt
    22501 ccagagcaaa gcggccgcgg cgcgccattt tcggacaaac agagtcggga cggcggccag
    22561 aaccagaagc cagaaatccc agcggccccc catatagatg ttccaagcct gtcccgctga
    22621 cgcccgcagg gagccgtaaa ctgcgccata acgcaggagg tgctgcaggt cgccaaaatg
    22681 aaagcctgca taaaaagcgc agccggccga caggggcagc gcgaaaaaaa gtgacgactt
    22741 tgtgcgtttc catagcgcca ggatcacgag aaaaggaaaa tagatggcca ctagatactt
    22801 gcagagaaac gccgcgaaga aacaagcggc cgccgccgcc caatcgcgct tccggttttc
    22861 ctgccagccg ccggagaatg cccagagtcc cagcgcgagg aaacaaatgg atcccgagtc
    22921 gcgggtcgcg attcgcgaaa ccagcactgc gggcgcaagc acggccatga cggccgccac
    22981 gcccaccgcc accgcgctgg aaaacaggcg gcgcgcgaaa ccgtagaccg ccgcaatcgt
    23041 caccatgccc agtccggccg ccatttcgcg cacggccgcc agtccaccga tgcgatcggc
    23101 aatggctgcc atggccggcc acagatacca tccaaagctc cattgtaggg gcgtgtcgag
    23161 gggcgcttca aaatggcggg agagaaacat ccggccatac acgacgtaaa tggattcatc
    23221 catgtatgcg gtcgtgtaaa aaggattatg aacgcgaaga aacagcgcgc ccgctgcaat
    23281 cagcatcagg gagagcactc gctcttgccg aaacagatcc gcgcgctccg gcagggcccc
    23341 ggccgctcct gacccgccac gcgttcccgc ggtgcgtgat tgggattctt ttgtcgtggg
    23401 tgttttcgcg gctccgccta gaagtagtgc cgccatgcaa atcggaacag gttccgttgg
    23461 aacgcgttaa aaacgtttaa gtacgcgtaa ctgaacgtga gttcattcat gggcgtcgct
    23521 ctccagaaga gatgcgtctc aaagctggag gcgaattgcg cgttggagaa ggaagagccg
    23581 ttgttctcaa cattctgtac gccggcggcc ccgctcatcc cccattgcac tgtcttgctg
    23641 aagcggtgcc caaccgtgag gatgccctcg ttgagcaaat aacgagtcgg gctgaaatat
    23701 cccccggtcg agcgcgataa gggctggcca ggctggacgt cggcatcatg attgaagctc
    23761 gagtaattca cgtagtaggc cacgcgcacg tagggctggt cgccgcgcag cgatttcccc
    23821 agttgagaat ccacggaaaa acgccggttg gacgccaggt tgcggcccgt gtaaacgccg
    23881 tccgtatagt ccagtgaaaa atcatactta tgagcggcgt tgttgtagct gattcccacg
    23941 tcccccaggt tggaatacac ttgcccgaag aacacaccgc cgaaattctg gccgttcgtg
    24001 gatagcagcg actcctccac cggctggcgt tgaaaattaa atttcagagt agtggaagaa
    24061 atgggcctga agctcagacc cacgccgccg tccacgttaa ccggaacgtt tcgaaacacc
    24121 tcggccccgg cctgctcatg cgtggtaact cggtcgctga gtgtggagtc gaggctggcc
    24181 ccaaagtaat tggaattggt gccctgcagg tgattgtcaa aaagagtggg ctgatacaga
    24241 aaagtgacgc ggttggacgg tccgagggtt gtcgagacag ccgcactgaa gcggtggaat
    24301 tccagattat tggctggctc ctggcggaaa gcgtagccga actccacgta ggttcctaag
    24361 ccgcgctgaa tctgttgccg cacgtcagcg aagtcaggcg tgggattccc gctcacatcc
    24421 gcgatggcct gtctggcctg ggcaaacctt tgcaaaccga tgttcgcgcg tgccagttcc
    24481 aggctggccc tcgtgtcgga aggcgctccc gtgtgcgcct gctgggcgag cgtgcgcgcc
    24541 tccacgaacc ggcccttcca gttcaggatc tcggcttcgc cccgcagcgc ggcggagttg
    24601 ttggggtcct tggccagcac ccgccggtac aactccagcg cctcgccggt gcggttctgc
    24661 caggcgatca actgcgcctt gccgttcagt gcccgcaggt tgtcggggtc cagtttcagg
    24721 acgcgttcac agcgcgccag ggcttcaccc cggctggcgc tgcgccagga gagcacctcc
    24781 gccgacacca gcagcagctc tatgtttttg ggatcgcgtt gcagcccgtt gtcgaagagc
    24841 gccaaggccc gtgcccgggt agcatcgttc cagctcagga tccgcgccag gcggatccgc
    24901 accgatgagg catccggatt cgcagagact atcgcctcta agttagtgac ggcttccgcc
    24961 cgggtctcct ggccccaact cagaatttca gcataggcgc cgcaaatctc cacatcgccc
    25021 ttgctggttt tgcaggcgcg gcgcaaaagc tccaaggcct cgtcatgtcc cccgggttgc
    25081 cagctcttca tccggcctag ccggtacagg gcggtggagt tttgcggttc cagtttcacc
    25141 agccgctcca acaacggcat ggcctcgcgc cactttccat cggcctccag cagatcggaa
    25201 agagaagaca atgcggcggc attttgaggg tcctgctcga gcagggcgcg atacatctgg
    25261 atggcttcat tggtgcggcc cgctgctgcc gcggcggctg cccgatcgag agttgcagtt
    25321 ccctgggcgg aagcattcac cgcgcctccc aggagaagca ggcaaaggag tgtggtcaag
    25381 ccgagggtct tcatgatcgt tctacctccg tctggctcgc atgagtcagg cgcagcacct
    25441 gaccttcgcg cggcgcgatc tgccagaaaa gaaacttgga tattttgtct tggtccgccg
    25501 cggtgatctg gtcaaaacgg atgccgctgg acatcctgct gtggccattc accgcataag
    25561 tccggttgcg gactaccttg ccggacgcct gaaccgtatg tccgggaagc ttgagttcaa
    25621 ccgtaatcgg tgtcccgcag acaattgcgc tttccattgt gagcgagacc ccaaagcggt
    25681 tcaggttgcg tgcaaattgc tcttggcgga cgagagttcc attcttgtcc ggatacgtca
    25741 cgcgcatccg caggtccagg cgcgccggga agcgataact ttcgcgcaac tccttgcgct
    25801 gcatcgaata cccgatcaca ccacccatca tgaacaggaa aacgagcgcc cagaagatgt
    25861 tcacggccag cgcccagaag aagtacccgt acagaggagt gtgctgtatg cggatgacac
    25921 ccaccaccag cgaagtcgcc aggccggcca ggagaatgag cggcaacaga acttcgggag
    25981 cgcgcgccgc ggaaccccgc ccctttggcg tgacgaagaa gccatccttc cggaagagcc
    26041 cgctaatcgt cttcatcagt acgtgcatct tcaatagatt gaactgttcg ctcaggaaga
    26101 aactgcgcca cccgccctgc agcaggatgg aacaggccag gttcaggccg aagtaaggca
    26161 ggaagtaact ggcgtacgac agcgggtccg tcaccatgac aaagacgccc aagtacagcg
    26221 agatgatggg cgtgatgacg aagaacagct tctggtagct catccaatag aagtacagcg
    26281 aggcgaaata acagatcttc tgccgcacgc ttaggccctt gctgaagaac gggttctccg
    26341 cccgcaggac ctgcatggac ccttggcccc agcgctgcca ctgccgggca aatccgctga
    26401 atgtttgcgg cgccagcccg cgcgccagcg tggagttgaa gtacagcacg cgccagcctt
    26461 tcttctgcag gcgcatgctg gtgtgcatgt cttccgtgat gctctgcgtc gggaatccgc
    26521 cgatatcttc cagcgacttg cgccgcacga cgcccgggct gccgcaatag aaggtagcgt
    26581 tgtggcggtc cttccccggc tggatgacgc tgaaaaaaag ctcctgctgt tgccatcccg
    26641 cccgcgtcct ccaattcacg cgatgttgaa aggaatccag gttgtaaaag tcctgcgttc
    26701 cctgcactac gcctaccctg ggatcggcga agaagccgat cgtattctca atcaattctg
    26761 gcgaagccac atggtcggcg tccaaggtga caatgaactc gccgctggtc tggcgtagcg
    26821 cattgttcag atttccggac ttggcgtcct tgtgatccgc gcgcctgaaa tagccgcaag
    26881 cgaattcctg cgcgagccgg cggacctctt cgcggcggcc gtcatccaga atgtaggtct
    26941 tgtgggggta ccgcatatta atggcgcaca tgaccgtttc gcgcaacagt tccagaggtt
    27001 cgttgtaggt gggaatgaaa acatccaccg agcgccccgg caacggctcc ggacgcgcac
    27061 ggtgcgtggg gttccaggtg ataaagtaga agaggaagct ctcgaaaaag ctgagcatct
    27121 ccgccaccag gaacgcgtaa aaaaagatct tcgccgccgg gttcatcgtg tgcaggatgc
    27181 gccagtaaaa gtagcgcact gtaaaaactg tcgccaagac caccccaatg cgggttacgg
    27241 ccagcttcag ctgacgggat ggcgggagtt tctcgacggg taatggctgg ctgcccgccg
    27301 cctgctttcc ttcaaccact atggacacct ccgggagctg tagtgtacga ggcgacagcg
    27361 ctcggacgat cttgccccat cgttttcatc ttcatcagtt tttgacgcac acttcggcgg
    27421 gccaacgggg aactagccgc caagaaccga gactgggcag tcagttctgc tgtccctcgc
    27481 cagtgaaacg ccctcgcgcc ggaaagcgca agggcggtgg taagcaaacc tccgcacctt
    27541 cacccaaact tcgggaactt ccctataagg ccaaagcgcg ggggtccttc ggcaaactgg
    27601 actagcctgc ccttgaatac gccaagtgtc aaggctttcc atcaagagga aaagtcttag
    27661 ggtttggaat ctcgttcggg accaacatga ggtcgttagt tctcttcggc ctgccgccct
    27721 ttgcgcggca gatccgatct taatcccacc tgctcggcga ttttccgcca gagagtgaga
    27781 attgtcagaa cgacgcgggg aagcttatct ctgtgtgact gtcagtttgt gatagccgac
    27841 cgtggcttct ttaattctat ccggcatctg aaattcagaa tcgagtccca cagtaggata
    27901 cttgggcttg agatatttgt tgataaagaa ctccagcgcc atcctgggga cgtcaaggcc
    27961 gtcaatattg acggtgcgca cttgtacttt gcctttgccg ccgctgccgg cggcgtctgc
    28021 ctcaacgcgg acgaaatgaa tgccgctaaa gatggaaagc agcgggttgg aggtgcgccg
    28081 tccggcgcgg atctcatcaa agtcaatcgt ggccagccct gtgattactc ccgactggcc
    28141 ctgcaaggta acttttttta ctccttgggg cagcttcacg cgccccgcag caatgtaatc
    28201 gttgatttct tcttccgtca tgatggtggg cacctggtca ggccgggccg aatcggcgtt
    28261 ctgtcggatg cggtccagct tacgttgcaa actatctgcc agccgatgtt cggaagaggg
    28321 ccgctggccg gaaaagccgg cgctgcaaag caagaaaaca atcgtggccg aagcagttct
    28381 cattgaatgc gtgcgagtcc atcctggtgc cgttccaact tggtgagccg aattgttgcg
    28441 cgcgattctt gcaaattgcg gcggacagga gaagctcgcc cgggcttagt cgtactaaga
    28501 cttcttgttc gctgatagcg gactctgtgg ccgcggctcc gcaatagagt tgatggtggt
    28561 cgcccgcaca aagcgttttt atgcgcagat cttgcccaat gttaattcat cgcccgcgac
    28621 ggatccaact tcccatccgc cccgctggtg agggagatga aagcagggtc cttagacagc
    28681 gagacaaaga cggcatccac cctggcctcc atcatctctt tgctgcgcac cgcttggtag
    28741 cgttcgtact cgaagaagtg ccgcttcagc agcgccagcg ccttttcctt ctgcccggcc
    28801 tgcgcgtaga ttgccgccaa attgtaagag gcgggcagga acgcctcgtt ctcgcgggca
    28861 atcttcagcg cctcttcggc atgtccgttg cctgccagga acgccgctgc gaaaacgagg
    28921 ttgtagggat ccgccggatc gagagccagc gcttcgcgcg tatagtggtc cgccttggcc
    28981 acgtcgcctt ccgagttcca atagacggcc aggttgcggt aggccagtgc cagcggatac
    29041 tcctttacgg aggcctccat cagcgccagt cccttcttga actccccttc gtggatcgtc
    29101 atcttcccca gtccatgcag cgccacggcg cgagacattg ggtcggccgg catcttcagg
    29161 attgcttcaa aggtcgtgcg ggccttgtcc caatcgccga tgtggtggcg atagagctcg
    29221 cccaggtagc gcaacggagc cggatcggcg ggatccatgc tgtgcgcctc ggtgtaaaga
    29281 cgctcagcgt ccgaatattt ggctgcgttc tccgccttca gaccgttacg catcacgcgc
    29341 tcgatctctg cgctcaattt gcgatcgaga attggcgcgt cgggaacctc ggcgacatcg
    29401 ttcaatccca gcttcttcaa ctccttggcc gcgtccttgg ctttgccggg gaacatgcac
    29461 ttctggtcat aaacggagcg atagagcgag atggctccgt ctttgtcgcc gctctttgct
    29521 ttttccctgg cctctctcat tttctccttg aggccctcat cgcgctgctt cacctcggtt
    29581 tccacgagtt tttccacctg gtccactctc aggaaaccgt ctttgtttgc ggccttcccg
    29641 atgaccttgc catccgcctg cgccagcacg gcgaccggca gcttgtcgcc gcccgcgtat
    29701 ttctgtccga tcggcgtgcg cgcgtccacg atgctcatcg tcacgcactg cgtggcgtag
    29761 agcgaaagcg tgcgcgag
//
LOCUS       DASYVO010000268         1915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_164117, whole genome shotgun sequence.
ACCESSION   DASYVO010000268 DASYVO010000000
VERSION     DASYVO010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164117"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="VGR03_08920"
     CDS             <1..1238
                     /locus_tag="VGR03_08920"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEV1994440.1"
                     /translation="GPVRKQAFLIATIYASFSLGSAAISQAGQARDGQPSSAQTPAAA
                     RPVGTIKSISGNTITLTTDAGSDVTVQVQEVTKLIRIAPGQKDLKDATPIQLADLQVG
                     DRILVRGKLANDGKSVVAASVIAMKKLDIAEKQAHDREEWQRHGFGGLVNSVDAASGT
                     IGVALPPQGERKSIAIHLSKDIILRRYALDSVMFDDAKPAPLDQIKPGDQLRARGTRS
                     ADGAEFTADEIVSGTFRNIAGTISALDASAGTITVQDLVAKKPITVRITPESQLRKLP
                     PQMAQRIAARLKGTTPEVPSSPTTPSAEQTARPSAPPTGGPGTGGSGGMGRSGGGGTA
                     DLQQMINRMPAAALADLQKGDAVMVVATESGTNGVSTVITLLGGVEPILEASPKSSAS
                     TILSPWSLSSGGGGEAATP"
     gene            1283..>1915
                     /locus_tag="VGR03_08925"
     CDS             1283..>1915
                     /locus_tag="VGR03_08925"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV1994441.1"
                     /translation="MSFRPARIVLLNLVFLLSIFPIGGNLEAQTPTGGLNGVVTDPSG
                     GVIAKAAVRLINASGASLDTTTNRDGFYEFKELVSGTYTVKAAAKGFAILTQEDVQIR
                     AGQIKQLNIGLLIQMEEEKVEVSDSSTKVDIAPSNNAGMVVMQGKDLEALSDDPDELQ
                     SELQTLAGPSAGPNGGQIYIDGFTAGTLPPKASIREIRINQNPFSAEYDKL"
BASE COUNT          434 a          565 c          554 g          362 t
ORIGIN      
        1 acgggcctgt gcgtaaacaa gcattcttga ttgcaacgat ttacgcaagt ttcagtctcg
       61 gaagtgccgc aatttctcaa gcggggcagg cacgcgatgg gcagccgtcg tccgcgcaaa
      121 cccctgcggc ggcgcggccc gtcggaacaa ttaagtccat ttccggaaat accattacct
      181 tgacgacgga tgcgggaagc gacgtgaccg ttcaagtcca ggaggtcacg aaactcattc
      241 gaattgcgcc cgggcaaaag gacttgaaag acgccacgcc catccagttg gcggacttgc
      301 aagtcggtga ccgcattctc gtgcgcggca agcttgccaa cgatggaaaa tccgttgtgg
      361 ctgcgtcggt gatcgccatg aagaagttgg acatcgcgga aaaacaggcc cacgaccgcg
      421 aagagtggca gagacacggt ttcggagggc tggtgaatag cgtcgatgcg gccagcggca
      481 cgatcggcgt tgctctgccc ccccaagggg agagaaagag cattgccatc catctttcca
      541 aggacatcat cctgcgtagg tatgctttgg attccgtgat gtttgacgac gcgaagcccg
      601 ctccactcga tcagatcaag ccgggtgacc agcttcgagc tcgcggcact cgcagcgccg
      661 atggcgccga atttactgcc gatgaaatcg tctccggcac gtttcgaaat atcgccggca
      721 caatttctgc gctcgacgca tcggcgggca ccatcaccgt ccaggatttg gtagccaaga
      781 aacccatcac cgtgaggatc acgccggaat cacagttgcg caaactgcct ccgcagatgg
      841 cacagcggat cgccgcgcga ctgaaaggga ctacacccga agtaccgtcg tccccgacca
      901 ctccgagtgc agagcaaaca gcgagaccca gtgctccgcc cactggcggc ccggggactg
      961 gcgggtctgg cggaatggga aggtccggcg gcgggggcac tgccgatctg caacagatga
     1021 ttaaccgcat gcctgctgca gcactggccg acttgcagaa gggcgacgcc gtcatggttg
     1081 tggcgaccga gagcgggacg aacggcgtct ccactgtgat taccttgctt ggtggagttg
     1141 agcccattct ggaggcttcc cctaagagca gcgcatccac aattctttcg ccatggagcc
     1201 tcagcagcgg aggcggcggg gaagcggcca caccgtaatg ctgggcagtc ctaaatcaaa
     1261 atatatttgc tctggagatc aaatgagttt tcggccagct cgaattgttc tattgaactt
     1321 ggttttcctt ctttcgattt tcccgattgg cggaaacttg gaggcgcaga cgcccaccgg
     1381 cggactgaat ggcgtggtca ccgacccctc cggcggagtc attgccaaag cggccgtccg
     1441 cctgatcaac gccagcggcg cgtcactgga caccaccacc aaccgcgacg gcttctacga
     1501 attcaaggaa ttggtttcgg ggacttacac cgtgaaggcc gctgcaaagg gcttcgcgat
     1561 tctcacgcag gaagatgtgc agatccgcgc cggacaaatc aaacagttga atattggact
     1621 cctcattcag atggaagagg aaaaagtgga ggtttcggac tcttccacga aggtcgatat
     1681 cgctccttcg aacaacgccg gcatggtggt catgcaaggc aaggacctcg aagcgctttc
     1741 ggatgatccc gacgaactgc aatccgaact tcaaaccctt gccgggccat ccgcgggccc
     1801 gaacggcggc cagatttaca tcgacggctt taccgccggc acgctccctc ccaaagcttc
     1861 catccgtgaa atccgcatca atcagaatcc gttctccgcg gagtacgaca agctc
//
LOCUS       DASYVO010000269         4153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_164166, whole genome shotgun sequence.
ACCESSION   DASYVO010000269 DASYVO010000000
VERSION     DASYVO010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164166"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VGR03_08930"
     CDS             complement(<1..291)
                     /locus_tag="VGR03_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994442.1"
                     /translation="MVLTTSTAIQLSAETQPDEEVTPVPLLTGSAGFITTFDGGEPHL
                     HPIVTPLVLVPIGRRWVFETRATFETDLVQLPGSSGFHGTVAKEVEYVQLDFI"
     gene            828..1760
                     /gene="pqqB"
                     /locus_tag="VGR03_08935"
     CDS             828..1760
                     /gene="pqqB"
                     /locus_tag="VGR03_08935"
                     /inference="COORDINATES: protein motif:HMM:NF003523.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HEV1994443.1"
                     /translation="MRIRVLGSAAGGGFPQWNCACPNCSALRDGKIKARPRTQAQVAV
                     SSDPSHWFLLNASPDLRQQILATPELSVTSSTLGSPISTILLTSADVDCVMGLLHLRE
                     FQPLHIYSTASVRRILTEENSLFRVLARSTPPVRWETLPLDRLIPLVPPSSAGAKDGL
                     FCKAVPLLGGFPDYVSDSLRGSLPPEEAVIGVQMVHKERRFFYAPSVAGLGEEWQRSV
                     DESDLALLDGTFWKEDELIQAKRSRRTARDMGHLPLWGERGMLKRPFRLGKTKRVIIH
                     INNTNPVLNEESPEHRAVREAGWEIAYDGMEFNL"
     gene            1757..2518
                     /gene="pqqC"
                     /locus_tag="VGR03_08940"
     CDS             1757..2518
                     /gene="pqqC"
                     /locus_tag="VGR03_08940"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR02111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HEV1994444.1"
                     /translation="MTPSVNHDVEKQLFSEAQFIAGFHAIGEERYHHKHPFHLLMHEG
                     KLTRGQLQAWALNRYYYQSRIPIKDAVILARSEDPAFRLAWRKRILDHDGDGIKPGGI
                     EKWLRLVEATGLAREHALRGDGILPATRFAVQAYIDFVSTCSHLEAVASSLTELFSHK
                     LIALRMDRMRQHYPWLSGGLDYFTGRLAQAPEDADFALAWVVKHARTRTEQNLAHAAL
                     RAKCDILWAQLDALYFAYVNPGWPPPGAFQPAKES"
     gene            2529..2810
                     /gene="pqqD"
                     /locus_tag="VGR03_08945"
     CDS             2529..2810
                     /gene="pqqD"
                     /locus_tag="VGR03_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HEV1994445.1"
                     /translation="MTPELNSFPRLAPGCRLNEKNQQPRTLLMPERALRLNGPSLEIV
                     ERCDGKHTVQQIIVDLQKIYSKAEPQKVEQDILGYLALLQKDRALDFEP"
     gene            2980..4086
                     /gene="pqqE"
                     /locus_tag="VGR03_08950"
     CDS             2980..4086
                     /gene="pqqE"
                     /locus_tag="VGR03_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HEV1994446.1"
                     /translation="MATENSNPLALIAELTHRCPLHCVYCSNPLELQTRTNELSTETW
                     SRVFNEAAEAGVLQADFTGGEPLARPDIVELVRAARSAGLYVNLITSGLPLDETKLAA
                     LVEAGLDHLQLSFQGAREDTANEISGAKSHAQKLRVLDWLRHHRVAVTLNFVIHRRNI
                     DQLEEMLALAESSCATRIEFANVQYYGWAFANRENLLPTRAQLDHSVELLRREQERLQ
                     GKIRIEFVVPDYYAKYPKPCMGGWGRKLLLITPSGDALPCHAAQVIPGLSFENVKDRS
                     LREIWEHSAAFEKFRGEDWMQEPCKTCDRREQDFGGCRCQALLLAGDAAATDPVCSLA
                     PLRPKVDAILAGVNSAPSAEIFAKPAWLYRPNPA"
BASE COUNT          853 a         1359 c         1068 g          873 t
ORIGIN      
        1 gatgaagtcg agttggacgt actcgacttc ctttgccacg gtcccgtgga atccgctgct
       61 tcccggcagt tgaaccaggt ccgtttcgaa tgtcgcccgc gtttcgaaga cccatcggcg
      121 tccgatcggc accagaacta gaggcgtgac aatggggtgg aggtgcggtt ccccgccatc
      181 aaatgtggtt atgaaacccg cgctccctgt aagcagagga accggcgtca cttcctcgtc
      241 tggctgcgtt tccgcactca actggatggc cgtagaggtc gtaagcacca cggcaagggc
      301 gatccaaaac gcgatttgcc tcccgggctt gttcctaaat cgacggactt cgcctccccg
      361 atgccgggat cggcttcgcc gaaaccaatt cgctgtggtg acttgcactt tcagtctcag
      421 agggtaagtc cccacctcaa gttaacgttg acccgcctcg cttttactgt caactcaagt
      481 gtataaaggt gtgctgcgaa ggataattcc cgtttggcgc gtccagcttc tgggcgtggc
      541 aaccctagta ccacccagga aggggccctt gccctccatc tccgccccag tcggaccagc
      601 aatccgttcc ggaatgaagt cgccaactcc gccattccct tcaacttaat tccatgttat
      661 gcttttgaag ttgtagtgta tttgactcca aaaggagcct ccaccatgga atgggttacc
      721 cctcagcacg aagaaatcga cctcaattgc gaagttagct cgtacgccaa cgccgagctc
      781 tagtctgaga gtcgaccccg accgggtcgg ggtcgccgga ttgccgaatg cgcattagag
      841 tcctcggctc cgcagccgga ggcggcttcc cccaatggaa ttgtgcttgt ccaaactgta
      901 gcgccctgcg cgacggcaaa ataaaggcgc gtccgcgcac ccaggcacag gtcgctgtct
      961 cttctgatcc gtcacactgg tttcttctga acgcctctcc cgacctgcgc cagcaaatcc
     1021 ttgcgactcc cgagttgtcg gtcacctcct cgaccctcgg ctcacccatt tcaaccatcc
     1081 tgcttaccag cgccgacgtg gattgcgtga tgggcttgct tcaccttcgt gagtttcagc
     1141 ccctgcatat ctattcgacc gcgtcggttc gacgcatcct gacggaagaa aacagtctgt
     1201 ttcgcgtcct cgcgcgatca actcctcccg tgcgctggga gactttgcct ctcgatcgtc
     1261 tcattcctct cgttccaccc tcttccgccg gcgcaaaaga tggcctgttc tgcaaagcgg
     1321 tgccgttact tggcggcttc cctgattacg tgagtgattc actgagaggc agcctgccgc
     1381 cagaggaagc ggttatcggc gttcaaatgg tgcacaagga aaggagattt ttctacgccc
     1441 ccagcgtcgc gggattgggg gaagagtggc aacgctccgt tgatgagagt gacctggctc
     1501 tgctcgacgg cacgttctgg aaagaggatg aactgatcca agccaagcgc agccgaagaa
     1561 cggcacgtga tatgggacat cttcccctct ggggcgagcg cggcatgctg aaacggccgt
     1621 ttcgtctcgg aaaaacaaag cgcgtcatca tccacatcaa caatacgaac ccggtgttga
     1681 acgaagagag tcccgagcat cgcgccgtgc gtgaagccgg ttgggaaatc gcctatgatg
     1741 ggatggagtt caacctgtga ctccatccgt gaaccacgac gtcgaaaagc agttgttctc
     1801 cgaagcccag ttcatcgccg gctttcatgc catcggagaa gagcgctatc accacaagca
     1861 tccgtttcac ttgctcatgc acgaaggcaa gctcacgcgc ggccagcttc aagcctgggc
     1921 tctcaatcgt tactactatc agagccgtat ccccatcaaa gacgccgtca ttctagcccg
     1981 cagcgaagat cccgcattcc gtctcgcctg gcgcaagcgc atactcgatc acgatggcga
     2041 cggcatcaag ccgggcggca ttgaaaagtg gctgcgcctc gtggaagcta ccggcctcgc
     2101 tcgcgaacac gcgctccgcg gcgatggcat tcttcccgcc acccgtttcg ccgttcaggc
     2161 ttacattgac ttcgtatcca cttgctcgca cctcgaagcc gtcgcttctt ccctcaccga
     2221 actcttctcg cacaaactca tcgctctccg catggaccgc atgcgccagc actatccctg
     2281 gctctccgga ggtctggact atttcaccgg ccgtctcgcg caagcacccg aagacgccga
     2341 tttcgcgctg gcgtgggtcg taaaacatgc gcgcacgcgc acggaacaga atttggccca
     2401 cgccgcgttg cgcgccaagt gcgacatcct ctgggctcag ctcgacgccc tctattttgc
     2461 ttacgtcaat ccaggctggc cgcccccggg cgctttccaa ccggcaaagg aatcgtgaaa
     2521 gatttaggat gacgcctgaa ctcaacagtt ttccgcggct cgctcccggc tgccgcttga
     2581 atgaaaaaaa tcagcagccg cgcacgctgc ttatgccgga acgcgccctc cgcctgaacg
     2641 gcccaagcct cgagattgtc gagcgctgcg atggcaagca caccgtccag caaatcattg
     2701 tggatttgca gaagatctat tccaaagccg agccgcaaaa ggtcgagcag gacatcctcg
     2761 gctacctcgc tcttttgcag aaggatcgtg ctctcgattt cgagccgtag cgccggcatc
     2821 cttgccggct tcttttgatt ttgtagcggc cgccttctga ggcgggcgct tttggttctg
     2881 tagcagtccc gctctgatca agagcgcatt tcttatcgta gtttttggag tggggcagcc
     2941 ctgctcccgc ttttccggcc aatacgccgg cacaatcgca tggctacaga aaactcaaat
     3001 ccgctcgctt tgatcgccga actcacccac cgctgccccc tgcattgcgt ctactgttcc
     3061 aatcccctcg aattgcaaac tcgcacgaac gaactttcca ccgaaacttg gtcccgcgtc
     3121 ttcaacgaag ccgccgaagc cggtgtcctt caagccgatt tcaccggcgg cgaacctctc
     3181 gcccgccccg acatcgttga actggtccgt gccgcgcgct ctgccggcct ctatgtcaat
     3241 ctcatcacct cgggcctgcc cctcgatgaa acaaaacttg ccgctctggt cgaagccggc
     3301 ctcgaccacc tccaactgag ctttcagggc gcgcgtgaag ataccgccaa cgaaatctcc
     3361 ggcgccaagt cccacgcgca gaaactccgc gttctcgatt ggctcagaca ccatcgcgtc
     3421 gcggttacgc ttaacttcgt catccaccgc cgcaacatcg atcaactcga ggaaatgctg
     3481 gccctcgcgg aatcctcctg cgccacgcgc atcgaatttg ccaacgtcca gtattacggc
     3541 tgggctttcg ccaatcgtga aaatcttctg ccaacgcgcg cgcagctaga ccactccgtc
     3601 gagctactaa gacgggaaca agaaagattg cagggaaaaa tccgcatcga atttgtcgtc
     3661 ccggattact acgcaaaata tcccaagccg tgcatgggtg gctggggacg caagctattg
     3721 ctcatcacgc caagcggcga tgcgctcccc tgccacgccg cgcaagtcat tcccggcctc
     3781 tctttcgaaa acgtgaaaga ccgcagcctg cgcgaaattt gggaacactc tgctgcattc
     3841 gagaaattcc gtggagaaga ctggatgcag gagccttgca aaacctgcga ccgccgcgag
     3901 caggacttcg ggggctgccg ctgccaggca ttgctcctcg caggagatgc cgctgccacc
     3961 gatccggtct gttcgctcgc gccgctgcgc cccaaagtcg acgccatcct cgcaggcgta
     4021 aacagcgccc catccgcgga gatcttcgcg aagcccgcct ggctctatcg cccgaatccc
     4081 gcctaatccg ccgaagcggc ttgtgtttct cccagcctca tcaccacccg aaacaaaaag
     4141 aaaagactcc cca
//
LOCUS       DASYVO010000270         4265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_91875, whole genome shotgun sequence.
ACCESSION   DASYVO010000270 DASYVO010000000
VERSION     DASYVO010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91875"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VGR03_08955"
     CDS             <1..280
                     /locus_tag="VGR03_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994447.1"
                     /translation="GLLGPSYETPAEIRYLRAVGADLVGMSTVLEVIAARHSGIRVLG
                     ISCVTNAAAGILDQPLNHLEVLETAERVKGEFIGLLKAVIPRIAEAIA"
     gene            277..669
                     /gene="cdd"
                     /locus_tag="VGR03_08960"
     CDS             277..669
                     /gene="cdd"
                     /locus_tag="VGR03_08960"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1994448.1"
                     /translation="MREYENLISAARQARENAHAPYSNFRVGAALRATSGRIFGGCNV
                     ENPTYGLTVCAERIAIFKAISEGERGFDAISVVTDTDALTPPCGACRQLIWEFCGDVP
                     VIMANLKGKTEKIQMKDLFPKPFDSSHL"
     gene            687..2174
                     /locus_tag="VGR03_08965"
     CDS             687..2174
                     /locus_tag="VGR03_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV1994449.1"
                     /translation="METQNPFRRSIVVLAILDLALFSFQSVVSAQTPAPPKQKQAQKS
                     TGQMSAGDTVWDLVVAGGTVVTMDSSHRVFENGIVVVQGDMIVAVEPSAPAAIDKYMY
                     TAKKIVDAKGKLVLPGFINGHTHVPMTLLRGLKDDVTLEEWLTKFIFPAEAKNVNEEF
                     VRWGTRLAAAEQIRGGITTFADMYYFEDAIAEETKAAGMRGVLGETILDFPAPDNKNS
                     AAMLEYTERFLKRWQGDPLIHAAVAPHSMYTCSQKTLQDAAALARKYKAPILIHVAES
                     KKELTDSREKNGTTPVQYLDKIGILGPDVLAAHCIFVDETDRKILAQREVGCVHNPSS
                     NMMLASGVAPVIEERAAGVAVGLGTDGPAGSNNDLDLMEEMDLAAKLQKITKMDPQAL
                     GAKAVVEMATIEGARALHMEKEIGSLEVGKKADLILIGLDAPNAVPMYDVYAQLAYAL
                     KGSDVETVIIGGRVVMRDKKLLTVDEPAVIAKAKEFKNKIEASLK"
     gene            2186..2572
                     /locus_tag="VGR03_08970"
     CDS             2186..2572
                     /locus_tag="VGR03_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994450.1"
                     /translation="MNSGNNEVRAPQGNEVVKQSSGISASLGAIVSSLATMGCCLPLG
                     FAGALGAVGASAFLQRFRPWLLAFSVVLLGIGFWQQRRARLCAVKRSYLSAVLLWTAV
                     VLVVAMIVFPQEIAGFIADHFSWAAK"
     gene            2569..2769
                     /locus_tag="VGR03_08975"
     CDS             2569..2769
                     /locus_tag="VGR03_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994451.1"
                     /translation="MKKKTLAFGVAVVTLLLVAVYLRMDSSTPPGQEPLATLTIANFA
                     AFEETFDKSTEGPRLILLLSPT"
     gene            2809..3159
                     /locus_tag="VGR03_08980"
     CDS             2809..3159
                     /locus_tag="VGR03_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994452.1"
                     /translation="MQRNSDPLLRVLVVWEPMLPTDLRSPVHSTLARIPDHRARQFWD
                     PQHLVANELRRIAGENPGQPKPDCCVDRGFFWDDAILYAPHSKWRQTPTAVFWNGPVV
                     RVASALEKALQNRP"
     gene            complement(3170..3988)
                     /locus_tag="VGR03_08985"
     CDS             complement(3170..3988)
                     /locus_tag="VGR03_08985"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEV1994453.1"
                     /translation="MEGKVFSEQTKTVVLSRHGAGIVSQYTLSAEQELIIRRLDTNKE
                     AEVRVVGQLGAAGKSYTYGVAFLDPEMDLWGSKFPSMTDSEKEASRVLLQCSGCKTRE
                     LVQHSDLESDVYLVNEGIVRSCKSCGASTFWKRATDDASGEPVLVETAPPEPEIPAEP
                     AETLPAASLDPAPTPQPAARTENRRKHVRTKANFKACIRSYTFGDDIVTCEDISRGGL
                     RFKSRKQYVAKTEIEVAAPYSPGAHAIFVRAQIVHVVELKQERRFRCGVCYAKS"
BASE COUNT          882 a         1185 c         1315 g          883 t
ORIGIN      
        1 aggactcctc gggccgagct atgaaacgcc tgcagagatt cgctatctac gagccgttgg
       61 cgccgatctg gtggggatgt ccacggttct ggaagtgatc gctgcgcggc atagcggcat
      121 tcgcgtgctc ggcatctcct gcgttacgaa tgcggccgca ggaattctgg atcagccgct
      181 caaccacctg gaagtgctgg agacggcgga gcgtgtgaag ggggaattca tcggcttgct
      241 gaaagcggtc attccgcgaa ttgcagaggc gattgcatga gagagtacga aaacctgatt
      301 tcggccgcac ggcaggcgag ggaaaatgcg catgcaccgt attccaattt tcgcgtgggc
      361 gccgcgcttc gtgccacttc cggcagaatc tttggcggct gtaacgttga gaacccgacc
      421 tatggcctca ccgtctgtgc cgagcgcatc gccatcttca aagccatcag cgaaggggag
      481 cgcggcttcg atgccatttc ggtggtgacg gacaccgatg ctcttactcc tccctgcggt
      541 gcctgccgtc agttgatctg ggaattttgc ggcgacgttc ccgtgatcat ggcgaacctc
      601 aaaggcaaaa cggaaaaaat ccagatgaag gatttgttcc ccaaaccctt cgattcctcc
      661 cacctataga aagaggaaac ccgaaaatgg aaactcaaaa cccgttccgg cgaagcatag
      721 ttgttcttgc gatccttgat ctggctctgt tttcctttca atccgtggtt tcagcgcaga
      781 caccggcgcc accgaagcag aaacaggcac agaaatcgac ggggcagatg tcagcgggtg
      841 ataccgtttg ggatttggtg gtggcgggcg gcacggtcgt gacgatggat tcgtcgcatc
      901 gggtcttcga gaacggaata gtagtggtac aaggcgacat gatcgtcgcc gtcgagccgt
      961 ccgctcccgc agccatcgac aaatacatgt acaccgcgaa gaagatagtt gacgcgaagg
     1021 ggaagctggt gctccccgga ttcatcaacg gtcacacaca tgtgccgatg accttgctcc
     1081 ggggcttgaa ggatgacgtg acgctggagg agtggctgac gaaattcatc ttccccgccg
     1141 aagccaaaaa cgtgaacgag gagtttgtgc gctggggcac acgacttgct gccgctgagc
     1201 agattcgtgg cggaatcacc acctttgccg acatgtacta tttcgaggac gccatcgctg
     1261 aagagacgaa ggccgccggc atgcgcggcg tgctgggcga aaccatcctc gactttcccg
     1321 ctccggacaa taagaacagc gcggccatgc tcgagtacac ggaaagattc ctgaagcgct
     1381 ggcagggcga tccgctgatt cacgccgcgg ttgcgccgca ctcgatgtac acgtgctcgc
     1441 agaaaacttt gcaggatgct gccgcactgg cgcggaaata caaagcaccc atcctgattc
     1501 atgtcgcgga gtcaaagaaa gagttaaccg acagccgcga gaaaaatggc accacgccgg
     1561 tgcagtatct cgacaagatt ggaattctcg ggccagacgt gctcgcggcg cactgcattt
     1621 ttgttgacga gacggaccgc aagatcctgg cgcagcggga ggtcggctgc gtgcacaatc
     1681 cttccagcaa catgatgctg gcgagcggtg ttgctccggt gatcgaagag cgtgcggcgg
     1741 gtgtggccgt cgggttgggc acggacggcc cggcgggcag caacaacgat ctggacctga
     1801 tggaagagat ggacctggcg gcgaagctcc agaagatcac caagatggac ccgcaggcgc
     1861 tgggggcgaa ggccgtcgtc gagatggcca ccatcgaggg cgcgcgtgcg ctgcacatgg
     1921 aaaaggaaat tggttcgctc gaagtgggga aaaaagcgga cttgattctc atcgggctgg
     1981 acgcgccgaa tgccgtgccg atgtacgacg tctatgcgca attggcgtat gcgttgaagg
     2041 gaagcgacgt ggagacggta atcatcggcg gccgcgtggt gatgcgagac aagaaactgc
     2101 ttacggtgga tgaaccggcg gtgattgcca aggccaagga attcaagaac aaaattgagg
     2161 cctcgctgaa gtaagccagg aagaaatgaa ctcaggaaat aatgaagtgc gagccccgca
     2221 gggcaacgaa gtggtaaagc aaagctccgg catctccgca tccctcgggg cgattgtgag
     2281 ttcgctcgca acgatgggct gctgtcttcc ccttggattt gcaggcgcgc tgggggcggt
     2341 cggcgcgagc gcgtttctgc agaggttccg gccctggctc ctggcgtttt ccgtcgtgct
     2401 gctgggaatt ggattctggc agcagcgccg cgcgagactg tgcgcggtga aacgaagtta
     2461 tttgagcgcg gtgctgcttt ggacagcggt cgtgctcgtt gtggcgatga tcgttttccc
     2521 tcaggaaatc gcaggattca ttgccgatca tttttcatgg gccgcgaagt gaaaaagaaa
     2581 accctcgctt tcggcgttgc cgtggtcacg ctgcttcttg tggcggtgta cctgcgaatg
     2641 gattcttcca cgccgcccgg tcaagagcct ctcgccacgc tcacaatcgc gaatttcgcg
     2701 gcgttcgaag aaacattcga caaatccacg gaggggcccc ggctgatact ccttctttct
     2761 ccgacttgat caacctgtct gcggggggtc tccgcaatcg aacaactctt gcagcgcaat
     2821 tcggaccctt tgctccgggt gctggtggtg tgggagccga tgctcccgac ggacttgcgt
     2881 tcaccagttc atagcacgct cgcccgaatc ccggatcacc gcgccaggca gttctgggac
     2941 ccgcaacatc tcgtcgcaaa tgaacttcgc aggattgccg gtgaaaaccc gggacaaccg
     3001 aagcccgatt gctgcgttga ccggggattt ttctgggatg atgccattct ctatgcaccc
     3061 cattcgaagt ggcgacagac accgacggct gttttctgga acggcccggt cgttcgcgtg
     3121 gcttctgccc tcgagaaggc actccagaat cgcccctagc agcgcgccct caggacttgg
     3181 cgtagcagac gccgcagcgg aagcgtcttt cctgcttcag ttccacaacg tggacgattt
     3241 gcgcgcggac aaaaatagcg tgtgcgcctg gtgagtaggg agcagccact tcgatctcgg
     3301 ttttcgcaac gtattgtttc cggctcttga agcggagtcc gccgcgggaa atgtcctcgc
     3361 acgtcacgat atcgtcgcca aacgtgtaac tccggatgca cgctttgaaa ttcgccttgg
     3421 tgcgcacgtg cttgcgcctg ttttcggtgc gcgccgcggg ttgcggggtc ggcgctggat
     3481 ccagtgatgc tgccgggaga gtctcagcgg gctccgctgg tatctcaggt tccggcgggg
     3541 ctgtttccac cagaaccggt tcgccgctgg catcgtcagt tgctcgcttc caaaacgtcg
     3601 aggctccgca gctcttgcaa gagcggacaa tcccttcgtt gaccaggtac acgtccgatt
     3661 ccaggtcgct gtgttgaacc agctcgcggg tcttgcatcc gctgcactgg aggagcacgc
     3721 ggctggcctc tttttcggag tccgtcatgc tggggaattt ggagccccac aggtccatct
     3781 ccgggtccag gaaggccacg ccgtaggtgt agctcttgcc agcagcgccg agctgaccga
     3841 cgacgcgaac ctccgcctct ttgttggtat cgagccggcg gatgatcagt tcttgttctg
     3901 cagagagcgt atattgcgaa acgatgccag cgccgtggcg gctgagcacg acagtcttgg
     3961 tctgttcgga gaagaccttg ccttccatgt cgctacctac caacagaatt gcaatttctc
     4021 gcggaattcg gctgctgcga cgcacgcctt ctggaggcat tcccacccca cgctgaggtt
     4081 gcccaggaac tggcaagatg gtacttggat ttctccgccg aagtcactga ttttgtagac
     4141 gcctgttttt tcgcgctcaa caagcggggc gggctcagag accgaatgcc acccagggag
     4201 gagatagaac tcgagtgccg attcaagaat gcggaaattt caagccggga aaggctcatt
     4261 cggga
//
LOCUS       DASYVO010000271         6679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_91905, whole genome shotgun sequence.
ACCESSION   DASYVO010000271 DASYVO010000000
VERSION     DASYVO010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91905"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      <1..173
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            232..2409
                     /locus_tag="VGR03_08990"
     CDS             232..2409
                     /locus_tag="VGR03_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV1994454.1"
                     /translation="MKTIVLRSVFLLFAVSVLSFSSRAQNNSPDAHLSGRVTDASGYT
                     IAGAKVTAQAETAPAPAVSIFSAVDGTYALSLPPGLYRIRFERPAFVPREISLSLADA
                     GARTLDVKLEIAQLSENIVVTANVQPLEISQTPAPVDIVTLDQFRQRQPVSLADALQF
                     LPGATIARTGREGGLTTFFLDGGNSNFTKFFVDGTPLNEPGGFLNLSNQTLDNIDKLE
                     LVHGAESALYGTDAVVGVVQLFSHRGATRIPELNLLAEGGGYSSARGAAQLAGLLGAF
                     DYSLAGSYFQTAGQGSNDAFLNRSFTGNFGYRVNDSNQLRLIVRSNSSVAGIPGPILF
                     RPPNLSQFESLKLLLVHLSWEFRTGSHWVHRLSGYENRTLDTNANPPFFTSIDQFNRA
                     GLQEQSTYTFRTGAVAAGYDYEVENGYPSLITGFHARRNNQAGFLDARWLPIPRLTFS
                     AGARVEANTSFGTRVVPRAGLVYALHNAHGFWGDTRARFSYGQGFKEPALEQSFGSDP
                     CFPGNSTLRPERSQTYDAGIDQFLAGDRLRLSATYFNNRFRDLVSFTFDFAATPACPF
                     GTGTYFNTDLARARGVNLSGELRLRRWLSLDGSYSHDDSRVLESPNAFDPVQLPGNHL
                     LRRPVNSGSLILNTVYRHWNLNLTGYFTGIRTDSDFFGLGFTHNPGYARFDLATSYFL
                     ARGISFTARVTNLFDKQYQDALGFPALGRDFRLGLNYRFSGRN"
     gene            2476..3165
                     /locus_tag="VGR03_08995"
     CDS             2476..3165
                     /locus_tag="VGR03_08995"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HEV1994455.1"
                     /translation="MNAPEPILFCWSGGKDSAMALYTLLQQKQFQVVALLTTVTEGYD
                     RIAMHSVRRELLRRQADAIGLPLHEVFIPPQCVNPVYEARMEEALRLFYNHGVRRVAF
                     GDIFLEDLRAYREKNLAHIGMTALFPIWKRDARELIRHFHERHFRAVTACVDSKVLDP
                     SFAGRELDESFFRDLPPHADPCGENGEFHTFVYDGPIFQSPIPIRTGEVVNRDGFVFC
                     DLLPQMEEARK"
     gene            3162..3719
                     /locus_tag="VGR03_09000"
     CDS             3162..3719
                     /locus_tag="VGR03_09000"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HEV1994456.1"
                     /translation="MNAEMSQKENLLYRTLLALALIVLAAALRIAPHPWNFTPVGAMA
                     LFSGAVLKDRRLAFFFPLLALFLGDIFIGFHKLIPIVYASFLINVAIGLWLRDRRTIS
                     RITLATLLGAIQFFVVTNFAVWQFLSGFPHTASGLVACYIAGIPFFWNTLAGDAFYAT
                     LLFGSFAIAERFSPALRPSRQRVSS"
     gene            3794..5278
                     /gene="gatB"
                     /locus_tag="VGR03_09005"
     CDS             3794..5278
                     /gene="gatB"
                     /locus_tag="VGR03_09005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV1994457.1"
                     /translation="MAKSIPEESSLAPEVLAKYEPVIGLEVHVQLLTRTKIFCGCSTR
                     FGDPPNTNVCPVCLGLPGTLPVLNKRAVEMAIRASLALNCAVHEHSRFARKNYFYPDL
                     PKGYQISQYELPLATGGWLEVEVGGARKRIGITRLHLEDDAAKNLHEGFSQSATKTYV
                     DYNRGGTPLSEIVSEPDMRTPEEAYAYLTTLRQILLYTGVSDCNMEEGSLRCDANVSV
                     RLRGAKEFGTKVEVKNLNSFRYLQKALEYEIERHIGVLESGGRISQETRLWNQAESHT
                     ISMRSKEKAHDYRYFPEPDLLPVHVSAAWRDEVLRTLPELPEAKRARFVSSFGITPYD
                     AEVLTNSQALADYFETAVKAGAPGKAAANWMQTELLRGLKDSGKEITASPVSPAALAE
                     LVKLVESAKITGAIGKKVFATMFESGRSAAEIVAAEGLGAQVSESAIEQAAREVIEKN
                     SENVAKFKAGNEGVFKFFVGQVMKSTRGQANPQAVNGILRKLLS"
     gene            complement(5529..5972)
                     /locus_tag="VGR03_09010"
     CDS             complement(5529..5972)
                     /locus_tag="VGR03_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994458.1"
                     /translation="MAFGFDEGPDCFDFAGFADEEGTAHDAHERPAHELFFLPGAEFL
                     DGFVGGIAEEGEIEILLCFERGLGFDGIGAHAEDGDAELVEVFFCVAKLGRFDRSTGG
                     VSFGIEEEQDALAGEVFERDVFAFVGFEAEGGGFGTYFEHSSPSF"
     gene            complement(6140..>6679)
                     /locus_tag="VGR03_09015"
     CDS             complement(6140..>6679)
                     /locus_tag="VGR03_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1994459.1"
                     /translation="FYEAPMDITLDKWDTAMDSQAKAFLVAVREAAAMMWKGGRIVAI
                     SFAPGGRFGSWQPWVAMGAAKAAMEVLVRYFAVALASRGITVNTISPGWVEDSVLNSL
                     PEAFQTGLREWQERGWTPMGRLGTPSDIGNAVAMICSEEASWITGQLINVDGGAALVD
                     GHLPLEFQGIPKPRAAGAA"
BASE COUNT         1329 a         2342 c         1602 g         1406 t
ORIGIN      
        1 aagaagccgt cgccgccgcg cgactgtaag gcggattaag tgaatgcgca ggccactgcg
       61 gctaatcgct ccatcgctgg gcgacgctgc gggaaggccc cgcaacggtc ctgattagaa
      121 aggaccggag ctaccgccaa gccaggagac cggcgaagtt cgcagccgtg cgatggacct
      181 tgatcgccag gacaaccttc aactcccgcg cgagagggag cggaggataa aatgaaaacg
      241 attgttcttc gtagtgtttt tcttctcttc gccgtctctg tactcagttt ttcctcgcga
      301 gcacaaaaca attcgcccga tgcgcatctt tccggccgcg tcaccgatgc ttctggctac
      361 actatcgcgg gcgcgaaagt cacggcacag gcagaaaccg cgccggcgcc ggcggtttca
      421 attttttccg ccgtcgatgg cacttacgct ctttcgttgc ctcccggtct ctaccgcatt
      481 cgcttcgagc gccccgcatt cgtcccacgg gaaatctcgc tttcgttggc tgacgcaggc
      541 gcccgcaccc tagacgtcaa actcgagatc gcgcaacttt ctgaaaacat cgtcgtcacc
      601 gccaacgtcc agccgctcga aatcagccaa acgcccgctc ccgtcgatat cgtcacgctg
      661 gaccaattcc gccagcgcca gcccgtctcg ctcgcggacg ccttgcaatt tcttcccggc
      721 gccactatcg ctcgcaccgg ccgcgaaggt ggcctcacca ctttcttcct cgacggcggt
      781 aactccaact tcaccaagtt cttcgtcgac ggcacgcccc ttaacgagcc cggcggtttc
      841 ctcaatctct ccaatcaaac gctcgacaac attgacaagc tcgaactcgt tcacggcgcc
      901 gaaagcgcct tgtatggcac cgacgccgtc gtcggtgtcg ttcaactttt cagccatcgt
      961 ggcgccacgc gcattcccga actcaatctc ttagccgagg gtggcggcta ttcctccgcg
     1021 cgtggcgctg cgcagctcgc cggccttctc ggtgcttttg attactcgct cgccggctcc
     1081 tatttccaaa ccgctggcca gggcagcaac gacgcttttt tgaatcgcag tttcacgggc
     1141 aatttcggct atcgcgtcaa cgactccaat cagttgcgcc tcatcgtccg cagcaactcc
     1201 agcgtcgccg gcattcccgg ccccatcctc ttccggcccc ccaacctctc gcagtttgag
     1261 agtctcaagc tgctgctcgt ccacctaagc tgggaattcc gcaccggttc gcattgggtc
     1321 catcgcctaa gcggctatga aaaccgcacg ctcgatacca atgcgaaccc gcccttcttc
     1381 acttccatcg accaattcaa tcgcgccggt cttcaggaac aatctactta tacttttcga
     1441 accggcgccg ttgcggccgg ttatgactac gaagtcgaga atggctatcc gagcctcatc
     1501 accggctttc acgcccgccg caacaatcaa gcaggcttcc tcgatgctcg ctggcttccc
     1561 attccgcgtc tcacgttcag cgccggggcg cgcgtcgaag ccaacacttc tttcggcact
     1621 cgcgtcgttc cccgcgccgg cctcgtctac gcccttcaca atgcccacgg cttctggggc
     1681 gatacccgtg cgcgtttctc ctacggccag ggcttcaagg aacccgccct cgaacagtct
     1741 ttcggcagcg acccctgctt ccccggcaat tccactcttc gccccgagcg cagccaaacc
     1801 tacgacgcag gcatcgacca atttctcgcc ggtgatcgcc tccgtttatc cgccacctac
     1861 ttcaacaatc gctttcgcga cctagtcagc ttcaccttcg attttgccgc cactccggcc
     1921 tgtccttttg gcaccggcac gtacttcaac accgatctcg ctcgcgcccg cggcgtcaat
     1981 ctctccggcg agctgcgcct ccgccgctgg ctcagcctcg acggcagcta ttcgcatgac
     2041 gattcgcgcg tcttggaatc ccccaacgcc ttcgaccccg tccagctccc cggcaatcat
     2101 cttcttcgcc gtcccgtcaa ctccggcagt ctcatcctca acaccgttta tcgccattgg
     2161 aatctgaatc tcaccggcta cttcaccggc atacgcaccg acagcgattt tttcggtctt
     2221 ggcttcaccc ataaccccgg ctacgcgcgc ttcgatctcg ccaccagtta tttcctcgct
     2281 cgtggcattt ccttcaccgc tcgcgtcacc aacctcttcg acaagcaata tcaggacgcg
     2341 ctcggctttc ctgcgctcgg ccgtgatttc cgcttgggcc tcaactaccg cttcagcggc
     2401 cggaactgac catggcgcca tttttcaatg cacttttctt actgagaact gaaaactgat
     2461 aaccgaccac ggcaaatgaa cgcacccgaa cccatcctct tctgctggag cggcggcaaa
     2521 gacagcgcca tggcgctgta caccctgctc caacagaagc aattccaggt cgtcgccctt
     2581 ctcaccaccg tcaccgaagg ctacgaccgc atcgccatgc acagcgttcg ccgcgaactt
     2641 cttcgccgcc aagctgacgc cataggcctg ccgctccacg aagtcttcat cccgccgcaa
     2701 tgtgtcaatc ccgtctacga agcgcgcatg gaagaagctc ttcgcctctt ttacaaccat
     2761 ggcgttcgaa gagtcgcctt cggcgacatc ttcctcgaag atctccgagc ctaccgcgag
     2821 aagaatctcg cccacatcgg catgaccgcc ctcttcccca tctggaaacg cgatgcgcgc
     2881 gaattaatcc gccatttcca tgagcgacat ttccgcgccg tcactgcttg cgttgattcg
     2941 aaagttctcg atcccagctt cgccggccgc gaactagacg aatccttctt ccgcgatctt
     3001 ccgccccacg ccgacccctg cggcgaaaac ggcgagttcc acacctttgt ctacgatggc
     3061 ccgatttttc aatctcccat tcccatccga acgggagaag ttgtgaaccg tgatggcttt
     3121 gtgttttgcg atctattgcc gcaaatggag gaagcaagaa aatgaacgcc gaaatgtctc
     3181 aaaaagaaaa tctgctctat cgcacgctgc tcgctctcgc cctgattgtg cttgccgccg
     3241 cattgcgtat cgccccgcat ccgtggaatt tcacgccggt tggcgccatg gcgcttttct
     3301 ccggcgcggt cctcaaggac cgccgcctcg cgtttttctt tcccctgctc gctctcttcc
     3361 tcggcgacat tttcatcggc ttccacaaac taatccccat cgtctacgcc agcttcctca
     3421 tcaacgtcgc catcggcctc tggctacgcg atcgtcgcac catctcccgc atcactctcg
     3481 ccacgctcct cggcgccatc caattctttg tcgtcaccaa tttcgccgtc tggcaattcc
     3541 tgagcggctt tccacatacc gcctccggcc tcgtcgcctg ttacatcgcc ggcatcccgt
     3601 ttttttggaa caccctcgca ggcgacgcct tctacgcgac cctgctcttc ggcagcttcg
     3661 ccattgcaga gcgtttcagt ccggctttgc ggccctccag acaacgcgtg tcatcctgag
     3721 cgaagcggtt ttgcagacgt tgtaagggcg aactttagtt cgccctacgc tctgctataa
     3781 tctacgttcc cctatggcga aatccattcc agaggaatcg tcactcgcgc ccgaagtgct
     3841 cgcgaaatac gagcccgtca tcggcttaga agtccacgtc cagcttctca ccaggacaaa
     3901 gatcttctgc ggctgctcga cgcgctttgg ggatccgccg aacaccaacg tctgcccggt
     3961 gtgcctcggc ttgcccggaa ctctgcccgt gctgaacaag cgcgccgtag aaatggccat
     4021 tcgcgcctcg ctcgccctga actgcgcggt tcacgagcat tcgcgcttcg cccgaaaaaa
     4081 ctatttctac ccggacctgc ccaagggcta tcaaatctcg caatacgaat tgcctctcgc
     4141 caccggtggc tggctcgaag tcgaagttgg aggcgccagg aagcgcatcg gcatcacccg
     4201 cctccatctc gaggacgacg ccgcaaaaaa tcttcacgaa ggtttctcgc agtctgccac
     4261 caagacttac gttgactaca accgtggtgg cacgccgctc agcgaaatcg tctccgagcc
     4321 ggacatgcgc acgcccgaag aggcctacgc ctacctcacc acgctgcgcc agatcctcct
     4381 ctacaccggc gtcagcgatt gcaacatgga agaaggctca ttgcgttgcg acgccaacgt
     4441 aagtgtgcgc ctgcgcggcg ccaaagaatt cggcaccaaa gtcgaagtaa aaaacctcaa
     4501 ctccttccgt tatctgcaaa aagcgctgga gtacgagatc gagcgccaca tcggcgtgct
     4561 cgaatccggc ggacgtatct cgcaggagac acgcctctgg aatcaagccg aaagccacac
     4621 catctccatg cgctcgaaag agaaggccca cgactatcgc tatttcccgg agccggacct
     4681 tctccccgtc cacgtcagcg cagcttggcg cgatgaagtg ctgcgcacgc tccccgaact
     4741 tccggaagcc aagcgcgccc gcttcgtcag ctcgttcggg attacgccct acgacgccga
     4801 agtcctcacc aactcgcaag cgctcgccga ttatttcgaa accgccgtca aagcgggagc
     4861 ccccggcaaa gccgccgcca actggatgca gaccgaactt cttcgcggat tgaaagattc
     4921 cggcaaggaa attactgcga gcccggtttc gcccgccgcc ctcgccgaac tggtgaagct
     4981 agtcgaatcc gccaagatta ccggcgcgat cgggaaaaaa gttttcgcca ccatgttcga
     5041 atccggccgt agcgccgccg aaatcgtcgc cgccgaaggc ctcggcgcgc aggttagcga
     5101 atccgccatc gaacaggccg cgcgcgaagt catcgagaaa aactctgaaa acgtcgccaa
     5161 gttcaaagcc ggcaatgaag gcgtcttcaa gtttttcgtc ggccaagtca tgaagtccac
     5221 gcgcggccag gccaacccgc aggccgtcaa cggcatcctg cgtaagctgc tctcctgacg
     5281 ccgcgcatct gtagctgggc ccagctgtag ctcaggcctt taggcctgag gcttttggac
     5341 tgcggcggct tgccgccgct tttcggtcgt cctcggcttc ttgacgccag aagcgtgttt
     5401 gtcattctga ggcgatccga taaggatcgc cgaagaatct caatttcgat ttcatcttgg
     5461 atatactcgt tgagagggaa tttgtagggg cgcagcatgc tgcgccccag ccagcaggat
     5521 gcagtttttt agaaagaggg acttgaatgc tcaaagtaag ttccaaagcc cccgccttcc
     5581 gcctcaaatc cgacgaaggc aaagacgtcg cgctcaaaga cttcgccggc cagcgcgtct
     5641 tgctcttctt ctatcccaaa gctaacacct ccggttgaac gatcgaagcg tccgagtttc
     5701 gcgacgcaaa aaaagacttc gacaagctca gcgtcgccat cctcggcatg agcgccgatt
     5761 ccgtcaaagc ccaagcctct ttcaaagcaa agcaaaatct caatttcccc ctcctcagcg
     5821 atcccaccca caaaaccatc caaaaattcg gcgcctggca gaaaaaacaa ttcatgggca
     5881 ggacgttcat gggcatcgtg cgcagttcct tcctcatcgg caaaaccggc aaaatcgaag
     5941 cagtctggcc cctcgtcaaa gccaaaggcc atcccgccga tgtccttctg cgcctttcgc
     6001 agaaataacc cgcaccggca atccgctgaa agaagtcccg ctttcagtcg tggattccgt
     6061 ccggccaagg ccactgatcg agccaagtac ttcacgaacc cccgcggaac cgggataccc
     6121 ccgtggggga agcgtctctc tacgctgccc ccgccgccct cggtttcggt atcccctgaa
     6181 attccagcgg cagatgcccg tccaccagcg ccgccccgcc gtccacattg atcagctggc
     6241 cggtgatcca actcgcttcc tcggagcaaa tcatcgcgac cgcgttgccg atgtccgacg
     6301 gtgttcccaa acgtcccatg ggagtccatc cgcgttcctg ccattcgcgc aatccggttt
     6361 ggaatgcttc cggcaagctg ttcagcacgc tgtcttcgac ccagcccggg ctgatcgtat
     6421 tcacggtgat gccgcggctc gccagcgcca ccgcgaaata tcgcaccagc acttccatgg
     6481 cagctttcgc tgcgcccatc gccacccatg gctgccagct tccaaatcgc ccgcccggcg
     6541 cgaatgagat agccacaatc cttccgccct tccacatcat cgcagctgcc tcgcgcacag
     6601 ccaccaggaa tgctttcgcc tgcgaatcca ttgctgtgtc ccatttatcc agcgtgatgt
     6661 ccatcggcgc ttcataaaa
//
LOCUS       DASYVO010000272         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_21450, whole genome shotgun sequence.
ACCESSION   DASYVO010000272 DASYVO010000000
VERSION     DASYVO010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21450"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VGR03_09020"
     CDS             <1..203
                     /locus_tag="VGR03_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994460.1"
                     /translation="ATGSASVEEVLLRLLETQEEQDRWLSENRETINAKIRRGIDQLD
                     RGQGIPEDHLDAYLADLKAKPE"
     gene            200..370
                     /locus_tag="VGR03_09025"
     CDS             200..370
                     /locus_tag="VGR03_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994461.1"
                     /translation="MTRRYVLVPEAALDLVQIWRFIKKQSSVEIADRVESVIRDKIVF
                     LATNPGAGHWRS"
     gene            396..1532
                     /locus_tag="VGR03_09030"
     CDS             396..1532
                     /locus_tag="VGR03_09030"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1994462.1"
                     /translation="MRRNSFSAVSSMIVVLLACAAVDLRAQAKPEMKKEEAPLRVALA
                     GLVHGHASGFFDQFQKRADLQVVGIAEADHQLAAQFAKSYGLAPSLFFSDLEEMLKAT
                     HPQAVLAYTNTYDHRRVVEVCARYGVHVMMEKPLAVSLEDARAIEKAAREGKIQVLVN
                     YETTWYRSNQAAYDLVHEKAIGEIRKIVVHDGHSGPKEIGVGPEFLAWLIDPKLNGAG
                     ALFDFGCYGADLATWLMDGRRPDSVTAVTQRIKPDVYPRVDDEATIILTYPNAQVIVQ
                     ASWNWPFSRKDMEVYGQKGYAITVGRDAVRVRLPEKEEISADAKPLEKTKEDSVSYLR
                     AVLLGGLKPEGQSSLETNVIVTEILDAARRSAATGKTVSLARTR"
     gene            complement(1553..>2350)
                     /locus_tag="VGR03_09035"
     CDS             complement(1553..>2350)
                     /locus_tag="VGR03_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1994463.1"
                     /translation="QIEQVIMNLVVNARDALPDGGRIVIETSNVDLDSTYTRDHAVVR
                     PGPYVLLAVTDTGIGMTAETVAHIFEPFYTTKESGRGTGLGLSTVYGVVKQSGGYIWV
                     YSEPGKGTTFKVYLPRVKDAVQVSESAEVPASTTRQGQETILLVEDEPAVRELTQMVL
                     SGRGYTVIEALTPEDAERLAVNNGAEIHLLLTDVVMPGMSGRELAKRLTGRYPNLRVL
                     YMSGYTYNVIAQDGTLEEGIFFLQKPFTPQALTQRVREALDRPVPAK"
BASE COUNT          497 a          653 c          730 g          470 t
ORIGIN      
        1 aggcaaccgg ctccgccagc gtcgaggaag tgctgctccg gctgctcgag actcaggagg
       61 agcaggaccg ttggctttcg gagaaccggg aaacgatcaa tgcaaagatt cggcgcggca
      121 tcgatcaact ggaccgcggc cagggaatcc ccgaagacca cctcgatgca tacctggcgg
      181 acctgaaagc caagcctgaa tgacacggcg gtacgttctt gtgccggaag ccgcgctcga
      241 cctagtccag atctggcgct tcatcaaaaa acagagcagc gttgaaatcg cggaccgcgt
      301 ggagtcggtc attcgggaca agatcgtttt cctggcgaca aacccgggtg ccggacactg
      361 gcgcagctga ggtagaatcc gacgcaagga ggagtatgcg acgaaattct ttttctgctg
      421 tttcctcgat gatcgtcgtc ctgctagcct gcgcagccgt tgatttgcgc gcgcaagcga
      481 agccggagat gaagaaggaa gaagcgccgt tgcgcgtggc gctggcggga ctggtgcatg
      541 gacacgcatc cggattcttc gaccaatttc agaagagggc ggacctgcag gtcgtgggaa
      601 tcgccgaagc cgatcatcag ctcgcggcac aatttgcaaa gagctacggc ctggcgccaa
      661 gtctcttctt ttccgatttg gaggagatgc tgaaagcgac gcatcctcag gcggtgctcg
      721 cgtacaccaa cacctacgat catcgccgcg tggtggaggt ctgcgcgcgt tacggcgtgc
      781 acgtcatgat ggaaaagccg ctggcggtca gcctggaaga tgcacgcgcg atcgagaaag
      841 cggcgcgcga ggggaagatt caggttttgg tgaattacga gacgacgtgg taccgcagca
      901 atcaagcggc ttacgacctg gtgcacgaaa aagctatcgg agagattcga aaaattgtcg
      961 tgcatgacgg tcatagcggc ccgaaagaaa tcggagtcgg gccggagttt ctcgcttggc
     1021 tcatcgatcc caaactaaac ggcgcgggcg cgctctttga cttcggctgt tatggcgcgg
     1081 acctggcgac ctggctgatg gatggccgca gaccggattc ggtgaccgcc gtgacgcagc
     1141 gcatcaagcc ggatgtctat ccccgcgtgg atgatgaagc gaccattatc ctgacttatc
     1201 caaacgcgca ggtgattgtc caggcttcct ggaattggcc gttctcccga aaagatatgg
     1261 aagtgtatgg acagaaggga tacgccatca ctgtcgggcg tgacgcggtg cgcgtgcggc
     1321 ttcccgagaa agaggagata tcggccgacg ccaagccctt ggagaaaacg aaagaagact
     1381 ccgtttccta tctacgagcg gtgctattgg gaggcctgaa gccggaggga cagtcttctc
     1441 ttgaaacaaa tgtaattgtg acggaaatac tcgatgcggc cagacggtcg gcggccacgg
     1501 ggaagaccgt ttccctggcg agaacacggt agagcgattg tgacgcgccc ttttattttg
     1561 cgggaacggg gcggtccagc gcctcgcgga ctctttgtgt gagcgcttgc ggtgtaaatg
     1621 gtttttgaag gaagaagatt ccttcctcaa gcgtgccgtc ttgcgcgatc acattgtacg
     1681 tgtagccgga catatacagc actcgcagat tgggataacg cccggtgagc cgcttggcca
     1741 gttcgcgccc gctcattccc ggcatcacca cgtccgtcaa gagcagatga atttcggcgc
     1801 cgttgtttac ggccaagcgc tccgcgtctt ccggtgtaag tgcttcgatc accgtgtacc
     1861 ctcgcccgga gagaaccatc tgcgtcagct cacgcaccgc gggttcgtct tcgacaagca
     1921 ggatggtttc ctggccctgg cgcgttgtgg atgcgggcac ttccgccgac tccgacacct
     1981 ggacggcatc tttgacgcgc ggtagataaa ccttgaaggt cgtgcctttt cccggctcgc
     2041 tgtacaccca gatgtagccg ccgctctgct tcacgacgcc gtacaccgtc gaaaggccca
     2101 gtccagtgcc ccgcccgctt tccttcgtgg tgtagaaagg ctcgaaaatg tgggcgaccg
     2161 tttcggccgt catgccgatg ccggtgtcgg tgaccgctag caagacgtaa ggtccgggac
     2221 ggacaaccgc gtgatcgcgc gtatacgtgg agtcgagatc gacgttgctc gtttcaatga
     2281 cgatccggcc gccgtcgggc aaggcgtcac gcgcattcac aaccagattc atgatgacct
     2341 gctcgatctg
//
LOCUS       DASYVO010000273         8902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_91944, whole genome shotgun sequence.
ACCESSION   DASYVO010000273 DASYVO010000000
VERSION     DASYVO010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8902
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91944"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..988)
                     /locus_tag="VGR03_09040"
     CDS             complement(125..988)
                     /locus_tag="VGR03_09040"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1994464.1"
                     /translation="MGIRFYCSGLAPFVLLLILEIASAPTGAQEIPLKHCDALPVIEV
                     TVGSQAMLFLVDTAATSILNLKSFAEGKTRAIEVTSWTGTLATSAREVTLREVAVGDT
                     KLTELKLPAVDLSAVGKACGRKIDGILGVDLLAKLGATIDLKKQTVHVTTLSEDRGDT
                     LAAEMQSEMHLCLVAFNESDENAFAECLDPKIVLFSGETELYGREQAIGYFRERYFHQ
                     KPAARLNIRESAFHAIGEAVWYEYEFTIESARGVLRGRGMAMCKKSEGRWRMASMHHS
                     VMEMDHSAAAK"
     gene            1069..1728
                     /locus_tag="VGR03_09045"
     CDS             1069..1728
                     /locus_tag="VGR03_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1994465.1"
                     /translation="MPKAAPLARRKKAPRRQARGLRTREAILARAVDIASVQGLEGLT
                     VGSLAENLHMSKSGLFAHFGSKQDLQLATIEAARTIFIKKVTRPALAAPKGMPRLWGL
                     LEQWLVLVEKRVFAGGCFFSAASFEFDSRRGKVHDRIAAIMHEWIAAITRAVYEAQKT
                     GHLDPKVDPTRLAFEIHAIAMGAHWASQLLDDRHAYSRARTIVLEKLRSIATPASPQL
                     P"
     gene            2165..3202
                     /locus_tag="VGR03_09050"
     CDS             2165..3202
                     /locus_tag="VGR03_09050"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV1994466.1"
                     /translation="MNRTNHAADDSTADGVTRREFLSAMLTTSILAGVAGPDLWAAGN
                     ENGIPYRKLGRTGERVSLVGLGGWHIGVQPDEQESAKIIRTALDSGINFLDNCWDYND
                     GRSEIRMGKALRDGYRQKAFLMTKIDGQTKKAAAAQIEESLRRLQTDYIDLLQFHEVI
                     RMTDPERIFSAGGGMEAVLEAKKAGKVRYIGFTGHKNPAMHLHMLETAANLEFHFDAV
                     QMPLNAMDAHYESFGQKVLPVLVKKEIGVLGMKPMGAGLLLRSNTLTPVECLHYAMNL
                     PTSVVITGCESVSNVQQALDAARSFKPLGSAEVAALLSRTEQAARDGQFEGYKTTNNF
                     DGTAHNPQWLG"
     gene            complement(3373..4650)
                     /gene="ispG"
                     /locus_tag="VGR03_09055"
                     /gene_synonym="gcpE"
     CDS             complement(3373..4650)
                     /gene="ispG"
                     /locus_tag="VGR03_09055"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEV1994467.1"
                     /translation="MLTQIEGFDATASRHPRRKTYGVNVGHVRVGGGAPIVVQSMTNT
                     DTADIETTLKQTLELAEAGSEIVRWTVNVPEAAAAVPEIKKRMLDAGCSAPIIGDFHY
                     NGHVLLTKFPECAKALDKYRINPGNVGAGQRRDEQFSTICKIAVDNGKPVRIGVNGGS
                     LNQELVMRKMQENTDKNLGKESEEIINDCMVLSALQSTELALESGLRHDQIIISCKTS
                     RPRDLITVYRDLSAKTEQPLHLGLTEAGMGMKGQIWSAASLGVLLYEGIGDTIRISLT
                     PKPGGDRREEVYAACELLQALGLRSFAPSVTACPGCGRTTSTTFQELAEAIQGYIRDS
                     MPIWKTQYEGVEEMTLAVMGCIVNGPGESKAANIGISLPGTGEAPRCPVYIDGQKDVT
                     LEGTYDELAVAFQALVDNYIAKKYPRKTVSVSA"
     gene            4784..6391
                     /locus_tag="VGR03_09060"
     CDS             4784..6391
                     /locus_tag="VGR03_09060"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HEV1994468.1"
                     /translation="MRILGLHRFLLLLLLALGVLPLTFGAEAPRLVQKDGRWALFVEG
                     RPYLILGGQIHNSSAWPSELPQVWASLAALHANTVEAPVYWEQIEPEPGQFDFANVDQ
                     LVKGARAHNLHLVLLWFGTWKNGNMHYAPKWVKTDPKRFPRVIKADGEPIDVLSAISR
                     ANLGADKAAFAALMRHLKALDGNEHTTLLVQVENESGSIGTVRDFSPEANAAFAGPVP
                     ADLLAVVHKEPGTWSQVFGSEADETFQAYYQAHYVNEVAAAGKAEFAIPVYINVWITY
                     PPAELPERRIPIPGIQYPSGGAVQKFVGLWRALAPSIDMIGPDIYSGESDFVQEIMRT
                     YRRPDNPLWIPETGRNDNFAKFLFYALGNGAVGFSPFGVDHSGWNALGDEIGKAHASN
                     FALLGPMSREIAKLNFEGKLKTSVEEPGHERQELDFGSWQATVSYGFPQHDGRRPPGT
                     KDAHGVALVAQLGPDEFLVTGVDASISFHLPGRLPGLRMQILSAEEGSYENGVWKPVR
                     LWNGDETDRGLSFHQEPAAVRILLGSF"
     gene            complement(6673..7170)
                     /locus_tag="VGR03_09065"
     CDS             complement(6673..7170)
                     /locus_tag="VGR03_09065"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEV1994469.1"
                     /translation="MKKNSPPLDLNGTGYCASFNFRRTARAVTRLYDMALQESGIRST
                     QFAILVGIAKSQPVSIGALADVLVIDSTTLTRSLRLLQKEGLVTVSKRAAMRQRFLTV
                     TAKGERTLARSLPAWRAAHERFVATIGSEYWDGMRSELERLAHVAVDLEKPEKDVAAL
                     MPANS"
     gene            7330..7914
                     /locus_tag="VGR03_09070"
     CDS             7330..7914
                     /locus_tag="VGR03_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEV1994470.1"
                     /translation="MSMQLVPMHSSEEHEGSHNAAVAPSRYVCFGAFHLDLKKEELFK
                     DGARVKLQGKVYQALLALLQKPGEIVTREELRLQLWPSDTHVNYDANVNTTVNKLRQV
                     LGDSPVQPAFVDTIPRKGYSFVAKIEYVDQPVTPPAPQPAERSLLAAVQERLESRASF
                     FRTVILSRWFTAGVVTLVIASMLFGAALVLYAHR"
     gene            complement(7966..8547)
                     /locus_tag="VGR03_09075"
     CDS             complement(7966..8547)
                     /locus_tag="VGR03_09075"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEV1994471.1"
                     /translation="MPTREDAWKLLCECTASESLRKHMLAVEACVRAYARKAGADEET
                     WSLAALLHDFDYERWPNQEHSPDQGHPSEGAKILREQGYSEEIVRAIITHADYCNVPR
                     QTALEHTLFACDEIAGFLTACAYVRPSKSILDLEVDSVKRRMKDKLFAKGVSREDMRK
                     GAEELGVPLEEHIAFCIAALREHTDALGLRGTL"
BASE COUNT         1821 a         2754 c         2445 g         1882 t
ORIGIN      
        1 tcgagataag ccagctccgc cacccgcaac acatcttccc ggctgatttt cacggatttc
       61 ctcggtgatt cgcgatacgc gtgcgaataa attctagcac atcgacgcac ggactccgcg
      121 gcccctactt ggctgccgcc gagtggtcca tctccatcac actgtggtgc atgctggcca
      181 tccgccatcg tccctcggac ttcttgcaca tcgccatgcc ccgccctcgc agtacccccc
      241 gcgcggattc aatggtgaac tcgtattcgt accataccgc ctcaccgatc gcgtgaaacg
      301 cgctctcgcg aatattcagc cgcgccgccg gcttctggtg aaagtagcgc tcgcggaagt
      361 agccgatcgc ctgctcgcgt ccgtaaagct ccgtctcccc gctgaagagt acgatcttcg
      421 ggtccagaca ttcggcaaag gcattttcat cggattcgtt gaacgccacc aaacacaagt
      481 gcatctcgga ttgcatttcc gcagccagcg tgtcgccgcg gtcctcgctc agtgttgtca
      541 cgtgcaccgt ctgctttttt aagtcgatgg tcgcgcccaa tttcgccagc aaatccacgc
      601 cgaggatgcc gtcgatcttc cgcccgcagg cctttcccac ggcggagagg tccacggctg
      661 gcaatttcag ctctgtcagt tttgtgtcgc cgaccgccac ttccctgagc gtcacttccc
      721 gcgcgctcgt cgccagcgtt cccgtccacg aggtcacctc gatagcgcgc gttttccctt
      781 ctgcgaacga tttcaggttt aagatcgagg ttgccgccgt atccaccagg aacagcatcg
      841 cctggcttcc aactgtcact tcgatcactg gcagcgcatc gcaatgtttc agtggaattt
      901 cctgtgcgcc cgtgggggca gacgcaatct ccaggatcag cagcaacacg aacggcgcga
      961 gcccactgca gtagaagcga attcccatgg cccacctcgg gtgcgggaaa gcctatcaca
     1021 aaatacgaac gttcgtgcta tttatttcgt tcatgttaca cttttttcat gcccaaggct
     1081 gcccctctcg cgcgcaggaa aaaagccccc cgccgccagg cgcgcggact tcgtacccgc
     1141 gaagccatcc tcgcccgcgc tgttgacatt gcctccgtcc agggcctcga aggcctcacc
     1201 gtcggctcac tcgcggaaaa cctgcacatg agcaaaagcg ggctttttgc tcattttggt
     1261 tccaagcaag atcttcagtt ggccaccatc gaagcagctc gcacgatttt cataaaaaag
     1321 gttacgcggc ccgccctcgc tgcgcccaaa ggcatgcctc gcttgtgggg tttgctggag
     1381 cagtggctgg ttctggtcga aaaacgcgtt ttcgccggag gctgcttctt cagtgcggcc
     1441 tcctttgagt tcgacagccg gcgcggcaag gttcatgacc gcatcgccgc catcatgcac
     1501 gaatggattg ccgccatcac ccgcgctgta tatgaagccc agaaaaccgg gcacctcgac
     1561 cccaaagtcg atcccacacg cttggctttc gagattcatg ccatcgccat gggggcgcac
     1621 tgggcttcgc agttgctcga cgaccgtcac gcctattcgc gcgcgcgcac gattgtgctc
     1681 gagaaattgc gcagcatcgc cacacccgcg tccccgcagc tcccgtaggc cagccggcaa
     1741 ttattccagc aagcgtgaaa gctttgggtc ggtgatgaat acagcgaagt cagaaccgaa
     1801 gatcacgcgt tcaagttacg gatcgatctt tccgtatttc gagaagctgt cgcggagggc
     1861 cctcagggcg caggatttcg gcccacggga cgcccagcct gcgatcttga acgctgatgg
     1921 cggcggggtc cctctgtaag gtctcgataa acgccgcatt ccgtccccgc gctggtcctt
     1981 ccgtggccac ccgcaaaatc acgcaagcca tcccataacc aatgcctcgc aatgtgtccc
     2041 tcatcggctt ctaccccctt tggcaagccc tgcactttcc ctcgtttcgt tcttacgcgc
     2101 aatcttcacc cttgcgcttc tggcctccgc ctcttacgct agcgactctg aggaggtgcc
     2161 caccatgaac cgcacgaatc acgccgccga tgattccacc gccgacgggg tcacccgccg
     2221 cgaattccta agcgccatgc tgacaacttc gatcctcgct ggcgtcgccg gcccggacct
     2281 ctgggccgcc gggaatgaaa acgggattcc ttatcgcaag ctcggccgca ccggcgaacg
     2341 cgtttccctc gtcggccttg gcggctggca catcggcgtc cagcccgacg aacaggagag
     2401 cgccaagata atccgcaccg ctctcgatag cggcatcaac ttcctcgaca actgctggga
     2461 ctacaacgac ggccgcagcg aaatccgcat gggcaaggcc ctacgcgatg gctaccgcca
     2521 gaaagccttc ctcatgacaa aaatcgacgg ccagactaaa aaagccgccg ccgcgcaaat
     2581 cgaggagtct ctccgccgcc tacagaccga ttacatcgat cttctccaat tccacgaagt
     2641 cattcgcatg accgacccgg agcgcatctt ctctgccggt ggcggcatgg aagccgtcct
     2701 ggaagcgaag aaagccggca aggtccgcta catcggtttc accggccaca agaatcccgc
     2761 catgcacttg cacatgctcg aaactgcagc gaatcttgaa tttcatttcg acgctgtcca
     2821 gatgcctctc aatgccatgg acgctcacta cgaaagtttt ggacaaaaag tccttcctgt
     2881 cctggtcaaa aaagaaatcg gcgttctcgg catgaagccc atgggcgccg gactcctcct
     2941 ccgcagtaac accttgaccc ccgtcgagtg cctccactac gccatgaacc tgcccaccag
     3001 cgtcgtcatc accggctgcg aatctgtgtc caacgtccag caagctctcg atgccgcgcg
     3061 cagtttcaag ccgctcggct ccgcggaagt cgccgccctc ctttccagga ctgagcaagc
     3121 cgctcgagac ggccaattcg aaggctacaa gaccaccaac aatttcgacg gcactgccca
     3181 taatccgcag tggctcggct agccaagatt attcgccact actctcggac tctgatctcg
     3241 tgcggagaat agagcactgt catcctgaag cgatccgata aagatcgctg aaggaactca
     3301 gcgttcactt caagcacaac gcttggatgg attgtgtgtt tgcttcagaa acgcgccgcc
     3361 ggaatccact gcttaagcgg aaacggagac ggtctttcgg ggatacttct tcgcaatgta
     3421 attgtccacg agcgcttgga acgcaactgc gagctcgtcg taagtgcctt ccagtgtcac
     3481 atccttctgc ccatcaatat agacggggca gcgcggcgct tcgccggttc ccggcaagct
     3541 gatgccgatg ttcgcagcct tcgactcccc cggtccgttt acgatgcacc ccatcaccgc
     3601 gagggtcatc tcctcgacgc cttcgtattg cgtcttccag ataggcatgc tgtcacggat
     3661 gtagccctgg atcgcctcgg ccaattcctg aaacgtggtg ctcgtggtgc gcccgcaacc
     3721 ggggcacgcc gtcacgctgg gcgcaaacga acgcagcccc agcgcctgaa gcaattcgca
     3781 cgcggcgtaa acttcttcgc ggcgatcgcc gcccggtttg ggcgtaagcg aaatgcgaat
     3841 cgtatcgcca atgccttcgt agagcagcac gcccagcgat gccgccgacc agatctgccc
     3901 cttcatgccc atgccggctt cggtgagacc caggtgcaaa ggctgttcgg tcttcgcgga
     3961 aaggtcgcgg tacacggtga tgaggtcgcg cggccgcgaa gtcttgcacg aaataataat
     4021 ctgatcgtgg cgcagtccag actccagcgc cagctccgtc gattgcagcg ccgaaagcac
     4081 catgcaatcg ttgatgatct cctcggactc tttgcccaga ttcttgtccg tgttctcctg
     4141 catcttgcgc atgaccagct cctgattgag cgagccgcca ttcacgccga tgcgcacggg
     4201 cttgccgtta tccacggcga ttttgcaaat cgtggagaac tgctcgtcgc ggcgttgtcc
     4261 cgcgccaacg ttacccggat tgatgcggta tttatccagc gctttcgcgc attcgggaaa
     4321 cttcgtcagc agcacgtgcc cgttgtaatg aaaatcgccg atgattggcg cgctgcatcc
     4381 agcatccagc atccgctttt taatttccgg taccgccgcc gccgcttccg gcacgttcac
     4441 cgtccagcgc acgatttccg aacccgcctc agccagctcc aaagtctgct tcaacgtcgt
     4501 ttcgatatca gccgtgtccg tgttcgtcat ggactgcacg acgatgggcg cgcctccgcc
     4561 cacgcgcacg tgtcccacgt tcacgccata ggtcttccgc cgcggatggc ggctggcagt
     4621 agcgtcaaat ccttcgatct gcgtaagcat ccctgttgat tctatcgtga tttgggccct
     4681 ccaggaaatc gagtcttctc ccgagtgatg caaactaccc tttttgatct aggaacagtt
     4741 gcatcgtatc ctgcacacgg ctatattgcg gggaggttct tacgtgcgca tacttggctt
     4801 acatcgcttt cttctactgc ttctccttgc cctcggcgtg cttccactca cctttggcgc
     4861 ggaggctccc agacttgtac agaaggatgg ccgctgggca ctcttcgtag aaggccgtcc
     4921 gtatttaatt ctgggtgggc agattcataa ctccagcgca tggccctcgg agctgccgca
     4981 agtgtgggcg tcgctggctg ccctgcatgc caacaccgtt gaagcgccgg tctattggga
     5041 acagattgaa cccgaaccgg gccagttcga ctttgccaac gtggaccaac tcgtgaaagg
     5101 cgcgcgcgcc cacaacctgc accttgtact attgtggttt ggcacgtgga aaaatggcaa
     5161 catgcattac gcgccgaaat gggtaaagac cgaccccaag cgctttccgc gcgtgatcaa
     5221 ggcagacggc gaacccattg atgtgctctc cgcaatttcg cgcgcaaatc taggggcgga
     5281 caaggcggcc ttcgccgcgc ttatgcgtca cctgaaggcg ctggatggca acgagcacac
     5341 cactctgctg gtccaagtag agaacgaatc gggcagcatc gggaccgtgc gcgacttttc
     5401 tccggaagcg aacgcggcgt ttgctggccc agttccggcc gacctgctgg ccgtcgtcca
     5461 caaagaaccc ggcacttgga gccaagtctt cggttccgag gcggatgaga ccttccaggc
     5521 ctactatcag gcgcactatg tcaatgaggt cgctgcggcg ggcaaagccg aattcgcgat
     5581 tccagtctac atcaacgtgt ggatcaccta tccacccgcc gaattgcccg aacggcgcat
     5641 ccccatccct ggcattcagt atcccagcgg cggggcagtg caaaagttcg tgggtctttg
     5701 gcgcgcattg gcgccttcga tcgacatgat cggtcccgac atctacagcg gtgagtccga
     5761 ttttgtccaa gagatcatgc ggacctaccg ccgcccggac aatcccctgt ggatacccga
     5821 gaccgggaga aacgataact ttgccaaatt tctgttctat gccctgggca atggagcggt
     5881 gggtttctct ccatttggtg tagatcatag cggatggaat gccctcggag atgaaatcgg
     5941 caaagcgcac gccagcaact ttgcgctact cgggccaatg agtagggaaa tcgccaagct
     6001 gaacttcgaa ggcaagctga agacatctgt agaggaaccc gggcacgagc ggcaggaact
     6061 ggattttggc tcctggcagg caacggtctc gtatggcttt cctcaacacg acggacggcg
     6121 gccgcctggt accaaagacg ctcacggagt cgccttagtt gcacaactgg gaccagacga
     6181 attcctagtg acgggggtag acgccagcat cagttttcac ttgcccggac ggctgccagg
     6241 cttgcgcatg cagattctct cggccgaaga ggggtcgtac gagaacggtg tttggaaacc
     6301 ggtgcggctg tggaatggcg atgagaccga tagaggctta agcttccacc aagaaccggc
     6361 ggctgtgcgg atcctgctgg gcagtttcta gggcacatcg ggaatgccct ggcttcagga
     6421 actcttcgtt atgccctagc gtgccatcgc acgctaggga tgttcttgcg ctatctttag
     6481 gaacatttgt gctgcagttt cgaatgcaaa ggaatgcaag cgattgagga tgggcttggc
     6541 agaaataata ttcgataagc ggcttgcgac gaagtgtgaa gcattgaagc agagcgagtc
     6601 cgcgggcgaa tgagacttcc actcccgact ccctgaacaa tttattccgg caatttgttc
     6661 tgaaccatgg aatcaagagt tggcaggcat cagggcagca acgtctttct ccggtttctc
     6721 caggtcgacg gccacatgag cgagtctttc caattcactc ctcatcccgt cccaatactc
     6781 ggagccgatc gtcgcgacga atcgttcgtg cgccgcgcgc caggcgggca agctgcgcgc
     6841 gagggtgcgc tctcctttgg ccgtaacggt caaaaaacgc tgccgcatgg cagcgcgttt
     6901 cgaaacggtg acgagcccct ctttctgcaa gagccgcaaa ctcctcgtca gtgtcgtgct
     6961 gtcgatgacc agcacgtccg cgagcgcccc gatggaaacc ggctgactct tcgcgatgcc
     7021 caccaatatg gcgaactgcg tcgagcgaat gccagattcc tgcagcgcca tgtcgtacag
     7081 ccgcgtgacg gcgcgcgcgg tcctgcgaaa gttgaacgaa gcgcagtagc ccgttccgtt
     7141 caggtccagt ggaggtgaat tcttcttcat tttccgcttc tcaagcttga cttgtatatg
     7201 catcttaaga tataacttac ctcaacgtga tgtcaagtgg ctgtatccat ggcccggtcg
     7261 ggcgccattc cgcgagtgga ataaatcgaa tgttgaatcg gtctgatgtt atgaccaagg
     7321 atgatttcca tgagcatgca actggttccc atgcactcgt cggaagagca cgaaggttcg
     7381 cataacgctg cagtcgctcc ctctcggtac gtctgttttg gcgccttcca tctggacttg
     7441 aaaaaagaag aacttttcaa ggatggggcg cgggtcaagc tgcagggcaa ggtctatcag
     7501 gcgctccttg cgctgctcca aaagcccggc gaaatcgtga cgcgcgaaga gctgcgcctg
     7561 cagctttggc cgtcggacac ccacgtcaac tacgatgcga acgtgaacac caccgtgaac
     7621 aagctccggc aagttctcgg cgattcaccg gttcagcccg cgttcgtgga cacgattccg
     7681 cgcaagggtt acagttttgt tgccaaaata gaatacgttg accaaccggt tacgcccccg
     7741 gcgccgcagc cggcggagcg ctcgcttctc gcagcggttc aggagcgctt ggaaagcagg
     7801 gcgtcgttct ttcggacggt tatcctttcc cgatggttta ccgctggcgt cgttactctg
     7861 gtgatcgcca gcatgctgtt cggcgcggca ttagttttgt acgcgcaccg ctgattccaa
     7921 acacccgcaa caataaatct tcgagcgagc cgtgcgcgga agccattaga gcgttccgcg
     7981 gaggcccaat gcgtcggtgt gctcccgcag agcggcgatg cagaaggcga tgtgctcttc
     8041 cagcggcacg ccaagttctt ccgcgccttt gcgcatgtcc tcgcggctga cgcccttggc
     8101 gaaaagtttg tctttcatgc ggcgcttcac cgaatccact tccagatcca aaatactttt
     8161 cgacgggcgg acataagcgc aagcggtcaa gaagccggcg atctcgtcgc aggcgaagag
     8221 cgtgtgctcc agcgcggttt ggcgaggcac gttgcaatag tccgcgtgcg tgatgatcgc
     8281 gcggacgatc tcctcggaat agccctgctc gcgcaaaatc tttgcgcctt ccgacggatg
     8341 cccctgatcg ggcgagtgct cctgattggg ccagcgctcg taatcgaaat cgtgcagcag
     8401 cgcagcgagg ctccaggttt cttcgtcggc gccggctttg cgggcatagg cgcggacaca
     8461 agcttcgacg gcgagcatgt gtttgcgcag gctctccgaa gcggtgcact cgcacagcag
     8521 tttccaggcg tcctcgcgtg tcggcatgcg gctctccccc aaatggagcg gatagtgtag
     8581 cgcaggggat aaggaagtcg acagttgaca gttgtaagtt gtaagtgagg aaagggaaga
     8641 cagaaatggg taacgcgggg acgtcccgac ccgatcggga tctcggcccc acaattgcgg
     8701 tggaacttcg atcggaacgt cgaagggagg agggggggta cacccccccc catgaaaatg
     8761 gaacgttatc aaaagaaagg agatgcgagg ggaacatttc gcaagtgatt gaaaggacag
     8821 atagatgcgc gacgggattt tgggtattcc taacgtttga gccaggatgg gctggttaac
     8881 cataacaccc ccacccccca tg
//
LOCUS       DASYVO010000274         4413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_188580, whole genome shotgun sequence.
ACCESSION   DASYVO010000274 DASYVO010000000
VERSION     DASYVO010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188580"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="VGR03_09080"
     CDS             complement(<1..1205)
                     /locus_tag="VGR03_09080"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV1994472.1"
                     /translation="MKSINLLLACIFASLLLATSVAQAQGVGSSGDIKGTVTDSSGSV
                     LPKVPVVAVETERGSRRTAMTDDSGEYRVTGLSPGTYDVSAKLPGFQTAIRRGVTVTI
                     GQTLIADFQLKVSSVATVVEVTGEAPLVDTEKSGQADTIEERYIRDLPINRRDYLSYT
                     LLVPGVSDSTRVASDVDFRVKQTPQSGLSFYGSNGRGNSVTVDGGEFNDDAGGVRLNL
                     SQDAVQEFQVNRSNYTAALGGASGASINIVSKSGSNQVHGSLYGFFRNDAFDARNPFS
                     VSQALQPGQAFNPNAPDTLSQPIKDSLNRQQFGGTVGFPVKKERTFLFLAFEGLRQNA
                     QNAVPLLTNTNIFRPQSDLLNNQVGILNALTARGAVPVPCLNGQPLIPAATCAGLLTS
                     ALTISPSTGL"
     gene            complement(1497..2516)
                     /locus_tag="VGR03_09085"
     CDS             complement(1497..2516)
                     /locus_tag="VGR03_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEV1994473.1"
                     /translation="MKAAVLQEFKKPLVIQEVARPTPGPEDILIEVEACGVCHSDLHV
                     ADGDWSQLAGIVKKPLILGHEVAGHVVEKGATVRDLHIGDRVGVPWIHWTCGECEWCR
                     GGNENLCPQQKITGVTVDGGYAEFVKAPASHVQRIPDGLSAHDAAPLFCAGVTVYRAL
                     HKSQLAPNQRLGIFGVGGLGHLAIQIGRALGAEVIAIDISDEKLALAESLGAASTLNA
                     LAKDVVQQLRGKGGVHIALVTSASKAAYDVAFYALRPTGTLLVVGLPAENICFPPILM
                     AAGEIHIQASAVGTREDLRQVLAMAAAGKVRCQVATRPLTDVNHVLEQLRCGRITGRV
                     VLLPH"
     gene            complement(2540..3256)
                     /locus_tag="VGR03_09090"
     CDS             complement(2540..3256)
                     /locus_tag="VGR03_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1994474.1"
                     /translation="MAVAKTKRAPRIRSRRSGGGLRGSARHGHAAQLAQPGSRDGLGL
                     RERKKARLRQQIIDTAIRLFRKNGYENTRVDDIVQVLEISQPTFFRYFPSKDAVLRDF
                     GLRGIECITEQIRSELAGDASTAERLRGLYGSLARQTEANRPLWQAVVLSGAMDPVRS
                     PEMREYDEVAVSLLREVLAQGQKRGEITREFPVVHLAEFMEGLYRTVVRHWAVDITGP
                     HKLTERVRSAVEFFLRGAQA"
     gene            3460..>4413
                     /locus_tag="VGR03_09095"
     CDS             3460..>4413
                     /locus_tag="VGR03_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEV1994475.1"
                     /translation="MSLVNHQFRLAAHPVGLPKRSDWTYTEEPVRGTVDGELLVKLLY
                     ISLDPAMRGWMNEGRSYIAPVGIGEVMRALALGRVIASKNSNFAAGDHVSGAFGVQEY
                     ALSDGAGVIKIDPRLAPLPVYLSALGMPGMTAYFGLLDTGQPKPGDTVVISAAAGAVG
                     SVAGQIAKIKGYRVIGIAGGAEKCQYVTRELGFDAAIDYKSEDVTKALQKHCPNAIDI
                     YFDNVGGDILNAVLTQLARGARIIICGAISQYNNAGPIKGPSNYLSLLMNRASMKGML
                     VLDYVNRYPEAVGAMAGWIASEKLKSREHIVEGLQTFPDTFL"
BASE COUNT          900 a         1346 c         1275 g          892 t
ORIGIN      
        1 agacccgtag acggagagat cgtcaaagcg ctggtcagca accctgcaca cgtggcggcg
       61 ggtatcaagg gttgtccgtt aagacagggc actgggaccg cgcctcgggc cgtcagtgcg
      121 tttagaattc ccacctgatt attcagcagg tcactctggg ggcgaaaaat attcgtgttc
      181 gtcaacagag gaactgcgtt ctgcgcattc tgtcgcaagc cttcgaaagc taggaacaag
      241 aacgtcctct ctttcttgac cgggaaaccc actgttcccc caaactgctg gcgattcaaa
      301 gaatccttaa tgggctggct tagcgtatcg ggtgcgttgg gattgaacgc ttggcccggc
      361 tgcagcgcct ggctaacgga aaatgggttg cgggcatcaa aagcatcgtt ccggaaaaag
      421 ccatacagac tgccgtgcac ctggttgctg ccgctttttg atacgatgtt tatagacgcg
      481 ccgctcgctc cacccaacgc ggccgtatag ttgctccggt tgacctggaa ttcctgcaca
      541 gcatcctggc tcaggttgag tcggacaccg ccggcgtcgt cgttgaactc gccgccgtcc
      601 acggtcacac tgttccctct gccattgctg ccatagaatg aaagtccgct ttgcggcgtc
      661 tgtttgaccc gaaaatctac gtcactagcc acccgggtgg aatcagagac accaggcacc
      721 agcaaagtgt agctaagata gtcacgccgg ttgatcggca aatcgcgaat atacctttct
      781 tcgatggtat ccgcctgccc acttttttcc gtgtccacca gcggcgcttc cccggttacc
      841 tcgactacgg tcgctactga cgacactttc aactggaaat ccgcaatgag cgtctgcccg
      901 atggtgaccg taacgcccct ccgaatcgcg gtttgaaagc caggcagttt tgcactcacg
      961 tcgtaggttc ctggcgatag ccctgtcact cgatactcac ctgagtcgtc ggtcatggcc
     1021 gtgcgccggg aacccctctc ggtttcaact gcaaccaccg gcacctttgg caggacgctg
     1081 ccagaggaat cggttaccgt tcccttgatg tcacccgagg agcccacacc ctgcgcctgg
     1141 gccacactgg tcgccaacag cagggaggca aaaatacacg ccagtaataa gttgattgac
     1201 ttcatgaaat ctccttctgg atgtgtgatg ttccgccatc ggatactgaa tttgagtgca
     1261 actttgggga ggctccgacc tgagcgcaaa gcggcaaacc catcatggtt gcgaaaagcg
     1321 cgagagcgag gacttggatg gtgcgccggc agccgcccag gaacacatgg cgactcacat
     1381 agcccatacg tcacccccgt ttcggtagtg gcagtcccac aattgtgtgt gtgactatca
     1441 cattagagtg actagcgtca agaggataag aaaggagggg tgggcgggga ttgcgatcag
     1501 tgcggcagga ggacgactcg cccagtgata cggccgcagc gcagttgttc gagtacatgg
     1561 ttcacgtcgg tgagcggccg ggtggcaacc tggcaccgaa cctttcccgc cgccgccatg
     1621 gcgaggactt gccgcaagtc ttcgcgcgta cccacggcgg aagcctggat gtggatttcg
     1681 cccgcggcca tgaggatggg tgggaaacag atgttctcgg cgggcaagcc aaccacgaga
     1741 agggtgcccg tcggccgcaa agcatagaag gccacgtcgt acgcggcctt cgaagccgaa
     1801 gtgacgagtg caatgtgcac accgcccttg ccgcggagtt gctggactac atccttcgcc
     1861 aaggcgttta gcgtactggc tgccccaaga gactctgcca acgcgagctt ttcgtcggaa
     1921 atgtcgatgg caatgacttc cgcgcccaac gcgcgcccga tctgaattgc caggtgtccc
     1981 aggccaccga cgccaaaaat gcccaggcgc tggttgggcg caagctggct tttgtggagc
     2041 gcgcgataga ccgtcactcc cgcgcagaac agaggcgcgg catcgtgggc agagaggccg
     2101 tcgggaatgc gctgcacgtg gctggccggc gccttcacga attccgcgta gccgccgtcg
     2161 accgtcacgc cggtaatttt ctgctgcggg caaagattct catttcctcc acggcaccac
     2221 tcgcactcac cgcaggtcca gtgaatccac ggtactccca cgcggtctcc gatgtgcaga
     2281 tcgcggaccg tcgcaccttt ttcgacgaca tgcccggcaa cttcgtggcc gaggatcagc
     2341 ggtttcttca cgattcccgc caactggctc caatcgccat ccgccacgtg gaggtcggag
     2401 tgacaaacgc cacaggcctc aacctcaatg aggatgtctt cggggccggg cgtggggcgt
     2461 gcgacttcct ggatgacgag cggcttcttg aattcctgca gaacggcggc cttcatcggt
     2521 ttgtcactcc ggggtacagc taggcttgcg cgccgcgcag aaaaaactct acggcgctgc
     2581 gcactcgttc cgtcagcttg tgcggcccgg tgatatccac cgcccaatga cgcacgacag
     2641 tgcggtagag tccctccatg aactccgcca gatgaaccac cggaaattca cgcgtgatct
     2701 cgccgcgctt ctgtccctgc gccagcacct cgcgaagtaa gctgacggcc acctcgtcgt
     2761 attcccgcat ctccggagaa cgcaccggat ccatggctcc gctcagcacc acggcctgcc
     2821 acagcggccg attggcttcc gtctggcggg ctaatgagcc gtacaaccca cgcaagcgct
     2881 cggcggtcga ggcgtcgccg gcaagttcgg acctgatctg ttcggtaatg cattcaatgc
     2941 cacgcaaccc gaaatcgcgc aggacggcgt ctttactggg aaaataacga aagaacgtcg
     3001 gctggctgat ctccaggacc tgaacgatgt catcgacccg ggtgttctca tacccattct
     3061 tccggaacaa ccggatcgcg gtgtcgatga tctgctggcg cagccgcgcc ttcttgcgct
     3121 cgcgaagccc aaggccatct ctgctccccg gttgggcgag ttgggcagcg tgtccgtgcc
     3181 gcgcagaacc ccgcagacct ccgccgctgc ggcgtgagcg gatacgcgga gcccgcttcg
     3241 ttttcgcaac agccatgtag atttcccccc gtatggaacg gatggcgtgc ctcacatagc
     3301 cagccgttga attataatag tcactataac gaatggaaca gtcactaacg atatatcaaa
     3361 ttgaatgaga tgtgcttggc accgtactcg catgcgcatc agatgttgca gcacatctcg
     3421 cttttagtat ctgagtaact gcatccggga gaattccgca tgagcctcgt caatcaccag
     3481 ttccggctgg ccgcccaccc cgttgggctg cccaaacgta gcgactggac gtacactgaa
     3541 gaacccgtgc gcggcactgt agacggcgaa ctgctggtga agctcctata catctccctg
     3601 gatccggcca tgcgcggctg gatgaacgag ggccgatctt atattgcgcc ggtgggcatc
     3661 ggcgaggtca tgcgcgccct tgcgctcggc cgcgttattg cctccaagaa ctccaacttt
     3721 gcggcaggcg atcacgtgtc cggcgcattc ggcgtgcagg aatatgcgct gtccgatggc
     3781 gcaggcgtca tcaagatcga tcctcgcctg gcgccacttc cggtatatct cagcgccttg
     3841 ggcatgcctg gcatgacggc gtatttcggc ctgctcgaca ccgggcagcc gaagccgggc
     3901 gacaccgtcg tcatctccgc tgctgctggc gcggtgggct cggtcgcagg gcagattgcg
     3961 aaaatcaagg gctaccgggt aattggcatc gccggcggcg cggagaaatg tcagtacgtt
     4021 acacgagagc tgggcttcga tgccgccatt gattacaaga gcgaagacgt caccaaggcg
     4081 ctgcagaagc attgtcctaa cgccatcgat atttatttcg ataatgtcgg cggcgatatt
     4141 ctgaacgccg tgctcaccca gcttgcgcgt ggcgcccgga tcatcatctg cggtgcgatc
     4201 tcgcagtaca acaacgccgg tccgatcaag ggaccgagca attacctctc gctgctcatg
     4261 aatcgcgcca gcatgaaggg catgctggtc ctagattacg tcaatcgcta cccggaagcg
     4321 gtgggcgcca tggccggctg gatcgcctcc gagaagctga aatcgcggga gcacattgtg
     4381 gaagggctgc aaactttccc cgacactttc ctg
//
LOCUS       DASYVO010000275         9572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_215961, whole genome shotgun sequence.
ACCESSION   DASYVO010000275 DASYVO010000000
VERSION     DASYVO010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9572
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215961"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1289..3607
                     /locus_tag="VGR03_09100"
     CDS             1289..3607
                     /locus_tag="VGR03_09100"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEV1994476.1"
                     /translation="MRKHLLKLASLLSVALAVALFAPQRAAADGDDPPSRVARLSYAQ
                     GTVSFNPGGTDDWVSAVVNRPITTGDKLWTDNGARAELHIGSAAIRLSSNTGFSFLNL
                     DDRMAQIRLTEGTLDVRVRRLDQDETFEIDTPNLAFSILRPGNYKINVNEAGDTTFVT
                     VRGGEGEVTGGGSAYTIHARETGTFAGIDQLNANIQSFGNGDDDFDRWSRDRDRREDR
                     SQSSRYVSSDVIGYEDLDDNGGWRRTPEYGTVWFPHTTIVGWAPYRYGHWVWISPWGW
                     TWVDDAPWGFAPFHYGRWVTVGGVWGWVPCPPRGEIGVEYVRPVYAPALVAWVGGPHF
                     SIGIGIGGGGGVGVAWFPLGPREVYEPSYHVSRTYVTNVNVSNTTVNNTVVNNYYNNV
                     IVNKNVTNITYVNQTAPNGVTATSQQNFSSAQPVGRNMMKVDRREVESAQVNATTPTV
                     APQQRSVLGSGAVSAARPPARLQERAVVAKTAPPPAPVSFVKQQQEIQANGGRPPAVS
                     EMRRVQTDNTPQDRPNIKIAPPSQPGTPRNVQANAPAPVQNNSRNRPGQPPAIEQNNS
                     NKPPVPANPGQPPANVQNNSNKPPAPTNADQQPQGNQPGNRPANNGRDKSYNDRPATA
                     RPNTANPMPPVNPQLDQKHQQQVDQLRQKQDQERQKIEQKQIQEQQRIQQKNAGDPRR
                     PQVDQKQQQQPQQIEQKPVQEPQRTQQTNAGDARRQQVEQKQQQQLQQLEQKHQQEQQ
                     KLEQKQQQDRQKQQEKPPSKPPKDEKKPPQRG"
     gene            3740..4540
                     /gene="cofE"
                     /locus_tag="VGR03_09105"
     CDS             3740..4540
                     /gene="cofE"
                     /locus_tag="VGR03_09105"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEV1994477.1"
                     /translation="MAKRSKSRSPSADIPAASPELRLFPIPGIPEIRAGNDLVKCVTA
                     AARRAAIHFEDGDILVFAQKIVSKAEGALVRLASVKPSPQALTIARRLKKDPRAIEVV
                     LRESRRIVRSDRVLIAETRHGFVCANAGVDHSNVPGKDVVTLLPRNPDRSAKKLAAAL
                     RKRTGKRVAVIISDTFGRPWRLGLTNVAIGASGVPVLLDLRGTRDRHGKTLTGTILAV
                     ADELAAAAGLAMGKSEGSPVVLIRGYRYKPSSEPAARIIRPAAEDLFR"
     gene            4617..5744
                     /gene="cofG"
                     /locus_tag="VGR03_09110"
     CDS             4617..5744
                     /gene="cofG"
                     /locus_tag="VGR03_09110"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HEV1994478.1"
                     /translation="MHTPLHPIARETALLLAGQAPLADLLSAATEVRAGGKGTVVTFS
                     KKVFIPLTTLCRDYCSYCTFRKDPGQPGAHFMTPVEVLALAERGRRAGCKEALFSLGD
                     QPEKIFPEAREFLRTQGYSRTLDYLAAMCELVLEKTGLLPHANPGVMDRAALVRLKDS
                     NASVGLMLENVSPRLMRDSLPHANAPDKVPALRLRTIEEAGKLSIAFTTGILIGIGET
                     MEERIDSLLAIRTLHEKYGHIQEVIIQNFRAKPDIPMAAHPEPSLDDMLRTIALARLI
                     LGPHMNVQAPPNLSYDDFPRLLEAGINDWGGISPVTKDFINPEAAWPQIARLQTETES
                     RGFTLRERLALYPEFLPRENFLAPRVRAKAELLAVEGGYAT"
     gene            5826..6770
                     /gene="cofD"
                     /locus_tag="VGR03_09115"
     CDS             5826..6770
                     /gene="cofD"
                     /locus_tag="VGR03_09115"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEV1994479.1"
                     /translation="MKPSLKITALAGGVGASKLLLGLYEEMDPTFLTIIVNTGDDIVL
                     HGLKISPDLDIVTYTLAGVVDSKKGWGLRAETFHALKHLVAYGRPKWFNLGDRDLATH
                     IHRTALLSEGKTLSQAADSIRIALRVKSRILPMSDSPVPTIIDSNEGPLHFQEYLVKR
                     RTEPVVKGIRFASVESAQPAPGVLEAIREADRILICPSNPLISIGPILAVPQIRDQLR
                     AQKEKIFAVCPIVGGKSLKGPSDKMLAQLGHKPTALGVAKLYTDFAGTFVIDPADKAQ
                     ASAIATLDMEVVVLPTVMKTLAQKRKLARTLLDMSYSL"
     gene            complement(6844..7332)
                     /locus_tag="VGR03_09120"
     CDS             complement(6844..7332)
                     /locus_tag="VGR03_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994480.1"
                     /translation="MTSRIVTKIGDIFEVPLDDQSKKYLQYIANDRTQLNSSVIRAFK
                     PKYTINALPAVNEITADAVDFYAHSVLKPGIIRKIWKKVGNSRDIGDLDVLFRGTNDI
                     LDPQIKISKNWHVWEIDGPSRRVGKLAGRNQKAEIGSVMPADQIVHRMRTGEYSFVYP
                     SY"
     gene            complement(7541..8218)
                     /gene="npdG"
                     /locus_tag="VGR03_09125"
     CDS             complement(7541..8218)
                     /gene="npdG"
                     /locus_tag="VGR03_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEV1994481.1"
                     /translation="MARKIAILGGTGAEGSGLAYRLAKAGEHILIGSREAARAQETAR
                     LLRERIGSAAQIEGMDNLSAAAAGDVAVLTVPFSGVVALLKQLKSVWKPGTIVVDTTV
                     PLAATVGGAATRMLGVWQGSAAEQTKELLPPGVGLAAALQNLGADSLAGDEPVDCDVL
                     VCSDDERAKQVASELVSKIPGARALNGGKLENARIVESLTALLIGLNMRYKVHGAGIR
                     FTGLPLK"
     gene            8339..8842
                     /locus_tag="VGR03_09130"
     CDS             8339..8842
                     /locus_tag="VGR03_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEV1994482.1"
                     /translation="MLTPPLTALEFRKALGHFTTGVTVVTVEREPGMIHGMTANSFTS
                     VSLDPMLILVCVDQRAKLLPLLEKKKRFGVSVLKAGQEAISEYFAKGEQSAEAEERLA
                     IRYRWTPSGVPVIEQTILQLSCNVVASHVAGDHTIFVGEVEDAEVHEGEPLLYFRGEY
                     RRIARHS"
     gene            complement(8900..>9572)
                     /locus_tag="VGR03_09135"
     CDS             complement(8900..>9572)
                     /locus_tag="VGR03_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994483.1"
                     /translation="GYDLVSGWRHNRRDHWLMRQLPSRTANWMMAKLSGIELHDFGTT
                     FKAYRREIIQEIQLYGELHRFIPALASSTGAKIAEVPIENLERKSGKSHYGIGRTIRV
                     FLDLIIVKFLLDYSTRPLQFFGLLGVAGAGLGSLLACFLAYEKFYLHKAIMAEHGPLM
                     MLAVALFISGVQFFSMGLLGEIIARTYYESQNKPIYSLREVKSHRKEMGDSAESTRPS
                     GNPDR"
BASE COUNT         2084 a         3110 c         2501 g         1877 t
ORIGIN      
        1 aacgtgccta cgatgaatag ccgttcgggc aacgtgcctg cgacatccca aatgattcct
       61 cgtcagcggg aactctcgca gaaccggccg ccttcgtcca tgacgaactc gaaccggaca
      121 tcagcctcta ctacgggcaa caatgcgtcg cagcgcgcgg gcaatagccc acgctcatgg
      181 gaagcgcaag gaaatgcaac ggatcgtggc cgcgcgcccg ccggtgtcgg aagcccgaat
      241 cagccgggaa gttcacgggc gcaagtaacc cgcgagactc aaggcaatcg tccgccatcg
      301 gcacgtggca attcgcagtc tggagccacc ggcggtcagg cctcacggcc ttcgacgccc
      361 agctacaaca gcaaccgccc aagctcttcc aacagcggtc gctcttacga gccgccccaa
      421 cggacaaacg gaaacacgcc gaactacggc aatcgcggcc cattaaacaa cggacgctct
      481 tacgaggcac cccagcggac gccgcgtgat acgccagcct acaacagcaa ccgcagctct
      541 tcgcagccgc gctcgtacga acctccgcaa cggacgcaac gtgatacgcc ggcctacaac
      601 agcaatcgcg cctattcgca gccgcgctcg tacgagccac cgcagcggac acaacgtgac
      661 acaccggctt acaacaacaa ccggggtaat tcgcagccgc gctcttacaa tccgccgccc
      721 acacgctcgt actcggcacc acaaacgtat tccgcaccga gccgcagcta cccggctccg
      781 tcacgctctt actccgcgcc cagcggtagc agcggcggcg gtggaaatcg gggcagcagt
      841 ggcggcggaa gcccgcacgg cggcgggggc tccgcccctc acggccggca ctaatctctt
      901 tgccaactta agagcccgtc cggatcttcc gggcgggctc tttctacttg gagttgatgc
      961 tctgacagtc tttcttcgtc gccaactagg cctgtaataa tctctcggtt ccgctctttc
     1021 gttctaattc tggtccttgc tgcagatccg aaagccgcca acgccactca gaaatacgtc
     1081 accagcaact ctttctcttg caaagtgtta aatagggtga cgcactccct ctcaggcgtt
     1141 tcctgtttcg acatgcaagg agggcctgat aggtgcatgg ccccattggg atgctactgt
     1201 gccaaaaggg atagagaccg gttaccaggg catgacagaa tgcggcgaat ataaaagacg
     1261 ccgcttgtga ttcaggagag atttcgatat gagaaaacat ctgctcaaat tggcgagcct
     1321 cctcagcgtt gcgctggcgg tggctctctt tgcgccgcaa cgcgccgcag ccgatggtga
     1381 cgatccgcca agccgcgtcg cacgcctgag ctacgctcaa ggcaccgttt cgtttaatcc
     1441 agggggcacc gacgattggg tttccgcggt cgtcaatcgt ccgattacca ccggcgacaa
     1501 gctgtggacc gacaacggcg cccgcgccga actgcacatc ggctccgcgg cgatccgcct
     1561 ttccagcaac actggttttt cgtttttgaa tctggatgac cgcatggcgc agatccggct
     1621 aacggaaggc actcttgacg tgcgcgtgcg gcgtctcgat caggacgaga ctttcgaaat
     1681 cgatacgccc aaccttgctt tctccatcct tcgtccgggc aactacaaga tcaacgtcaa
     1741 tgaagctggc gataccacct tcgtgaccgt tcgcggcggt gaaggcgaag tcaccggtgg
     1801 tggctccgct tataccattc atgctcggga aaccggcacg tttgccggga tcgaccaact
     1861 caatgccaac attcagagct tcggcaacgg tgatgacgat ttcgaccgct ggtctcgtga
     1921 ccgcgaccgc cgcgaagatc gctcgcaatc ctcgcgctac gtctcttccg atgtgattgg
     1981 ctatgaagac ctcgacgaca acggtggctg gcgccgcact cccgaatatg gcaccgtttg
     2041 gtttccacac accaccatcg ttggttgggc tccatatcgc tatggccact gggtgtggat
     2101 ttcgccctgg ggttggacct gggtagatga tgctccctgg ggctttgcgc cgttccatta
     2161 cggccgctgg gtgaccgtcg gtggtgtttg gggttgggtg ccgtgcccgc cccgggggga
     2221 gataggtgtt gagtacgtgc gcccggttta cgctcccgcc cttgtcgcgt gggttggcgg
     2281 accacacttc agcattggca taggaatcgg aggaggcggt ggagtcggcg tggcctggtt
     2341 cccgcttggg ccgcgtgagg tctacgagcc ttcttatcac gtaagccgca cttacgttac
     2401 aaacgtgaac gtctccaaca ccacggtgaa caacactgtt gtgaacaact actataacaa
     2461 cgtcatcgtc aacaaaaacg tcaccaacat cacctacgtg aaccagaccg ctcctaatgg
     2521 cgtaaccgcc acttcgcagc agaatttctc gtccgcgcag cctgtcggac gaaatatgat
     2581 gaaggtcgat cgccgggaag ttgagtccgc gcaggtcaat gccactaccc caacggttgc
     2641 cccgcaacag agaagcgtac tcggctccgg cgctgtgagt gcggcaaggc ctccagcgag
     2701 gcttcaggaa cgcgcggtcg tggccaagac agcgccgcca cccgcacctg tctctttcgt
     2761 aaagcaacag caggagattc aagccaatgg cggcaggccg ccggccgttt ctgaaatgcg
     2821 tcgagtgcag accgacaaca cgccacagga tcgtccgaac atcaagatcg ctccgccgtc
     2881 ccaacccggc acgccgcgaa acgttcaggc caatgctccc gctccagtac agaacaacag
     2941 ccgcaatcgt ccagggcagc cgccggcgat tgaacaaaat aattcgaaca agcccccggt
     3001 tcccgccaac ccgggccagc cgccggccaa tgtgcaaaac aattcgaaca agccccccgc
     3061 tcccaccaac gctgatcagc agccgcaagg caatcagcct gggaatcggc ccgcaaacaa
     3121 tggtcgagac aagtcctata atgatcgtcc ggctaccgct cggccgaata ccgcaaatcc
     3181 catgccccct gtgaacccgc agctcgatca gaaacatcag cagcaggtgg accagctccg
     3241 ccagaaacaa gaccaggagc gccagaagat cgagcagaag caaatccagg aacagcaaag
     3301 gattcagcag aaaaatgctg gggaccccag aaggccgcaa gtcgaccaga aacaacagca
     3361 gcagccccag cagatcgaac agaaaccggt tcaggagccg caaagaactc aacaaacaaa
     3421 tgctggcgac gctagaaggc agcaggtcga gcagaaacaa cagcagcagc tccagcagct
     3481 cgagcagaaa caccagcagg agcagcagaa gctggagcaa aaacagcagc aggatcgcca
     3541 gaagcagcaa gagaaaccgc ccagcaagcc gccgaaggac gagaagaagc ctccccaacg
     3601 cggctagccc ggctgtcttg cggacacaat cctgggtggg cgcttgctta tagtcaggcg
     3661 cccgaccgtt ttcttgattt cacgcatctt cgcaggtgaa agcttttgct gtgtcgcgta
     3721 agattccttt tccgcgctca tggcgaaacg atctaaatcc cgctccccat ccgctgacat
     3781 cccggctgca tctccggagt tgcggctatt tccaattcca ggcattccgg agattcgcgc
     3841 cggcaatgat cttgtcaaat gcgtcaccgc cgccgcgcgt cgagctgcca tccacttcga
     3901 ggacggcgac atccttgtct tcgctcagaa aattgtctca aaagcggaag gcgccctcgt
     3961 tcggctcgca agcgtaaagc cttcgccgca agccctcacg atcgccaggc ggctgaaaaa
     4021 agatcctcgc gccatagaag tcgtcttgcg ggagtcgcga cgcatcgttc gcagcgatcg
     4081 tgtgctgatc gccgaaacgc gccacggttt cgtctgcgcc aatgcaggcg tcgaccattc
     4141 gaacgttccc ggcaaagatg tcgtaacgct gcttccgcga aatccagatc gatccgcaaa
     4201 gaaactcgct gccgccctgc gcaagcggac cggcaagcgc gtcgccgtca tcatcagcga
     4261 tactttcggc cgcccctggc gccttggact gacgaacgtg gccatcggcg cgtcaggcgt
     4321 gcccgttctt ctcgatttgc gcggcacgcg ggaccgtcac ggaaagactc taaccggaac
     4381 gatcctcgcc gtcgccgacg agctcgccgc agccgccggt ctcgcaatgg gcaaatccga
     4441 aggctccccc gttgtcctca tccgcggcta tcgctacaag ccctcctcgg aacccgccgc
     4501 ccgcatcatc cgccctgccg ccgaagacct tttccgctag tctcctcttc tctgtgaact
     4561 ctgtgtcctc cgtgccctct gtgttaaatt cccttcgtac cgttgctcgc ttgcccatgc
     4621 acactccact ccatcccatc gcccgcgaaa ccgccctcct cctcgccggg caagctccgc
     4681 tagccgatct cctaagcgca gccaccgaag tccgcgctgg cgggaaggga actgtcgtca
     4741 cgttttcaaa aaaagtcttc atcccgctca ccaccctttg ccgcgactat tgcagctact
     4801 gcacctttcg caaggacccc ggccagcccg gcgctcactt catgacaccg gtcgaggttc
     4861 tcgcgctcgc cgaacgcggc cgccgcgcgg ggtgcaaaga agctctcttc agcctcggtg
     4921 atcaacccga aaagattttc cccgaagccc gcgaattcct ccgcactcag ggctattcgc
     4981 gcacgctcga ctatctcgcg gcgatgtgcg aactcgttct ggaaaaaacc ggcctgctcc
     5041 cgcacgccaa tcccggcgtc atggaccgcg ccgccctcgt gcgcctgaaa gattcgaacg
     5101 ccagcgtcgg cctgatgctg gaaaacgtca gcccgcgcct gatgcgcgat agtctgccgc
     5161 acgccaatgc tccggacaag gtgcccgcgc tgcgtctccg caccatcgaa gaggcgggga
     5221 agctctccat cgccttcacc accgggattt tgatcggcat cggcgaaacc atggaagagc
     5281 gcatcgactc tctcctagcg attcgaacgc tacacgaaaa atacggccac attcaggaag
     5341 tcatcatcca gaatttccgc gccaaaccgg atataccgat ggccgcgcac ccggagccat
     5401 cgctcgacga catgctgcgc acgattgccc tagcccgcct gatcctcggc ccgcacatga
     5461 acgtccaagc cccgccaaac ctgagctacg acgatttccc acgtttactg gaagcgggca
     5521 tcaacgactg gggcggcatt tctcccgtca caaaagattt catcaatccc gaagccgcgt
     5581 ggccgcagat cgcccgcctg caaaccgaaa ccgagtcccg cggcttcacc ctgcgcgaac
     5641 gcctagcgct ctatcccgaa tttctcccgc gcgaaaactt cctcgccccg cgtgtgcgag
     5701 cgaaagccga gctcttagct gtggaaggag gctacgcaac gtaggggcgg gcttccgccc
     5761 ggcgctttgg agtgcggcag ctcgctgccg cttttctttc tgctaccgcg tcccgcacac
     5821 caaccatgaa accatcacta aaaatcaccg ccctagccgg cggcgtaggc gcctcaaaac
     5881 tcctcctcgg tctttacgaa gaaatggacc caaccttcct aacgatcatc gtcaacaccg
     5941 gcgacgacat cgtcctccac ggcctgaaaa tttcgccaga cctagacatt gtcacctaca
     6001 cccttgcagg agtggtcgat tcaaaaaaag gctggggcct tcgcgccgaa acatttcacg
     6061 ccctgaaaca cctagtcgcc tacggccgcc ccaagtggtt caatctaggc gaccgcgacc
     6121 tagccaccca catccaccgc accgccctgc tgagcgaggg gaaaacgctg tcccaggccg
     6181 ccgattcgat tcgaatcgct ctgcgcgtga agtcccgcat cctcccgatg tcggacagtc
     6241 ccgttcccac gatcatcgat tcgaacgaag gccctcttca ttttcaggaa tatttagtca
     6301 agcgccgtac cgaaccagtt gtgaagggaa ttcgttttgc cagcgtggaa tccgcgcagc
     6361 ctgctcccgg cgtcctcgaa gcaattcgcg aagcagaccg catcctcatc tgccccagca
     6421 atccgctgat cagcatcggc ccgatccttg ccgtgcccca aatccgcgac caactccgcg
     6481 cccaaaaaga aaaaattttc gccgtctgcc ccatcgtcgg cggcaaatcc ctgaaaggcc
     6541 cctccgacaa aatgctcgcc caactcggcc acaaacccac cgctctcggg gtcgccaaac
     6601 tctacacaga cttcgccggc acgtttgtga tcgatccagc agacaaggca caagcctctg
     6661 cgatcgccac gctagatatg gaagttgtgg ttttgcctac tgtaatgaaa acactagcgc
     6721 aaaagcgcaa actcgcccgc accctcctgg acatgtctta ttctttgtag ggctgcagca
     6781 ccactgcgcg caagcttgtg cacgccgccg caactgttgc tatgtttgca caacgcctgc
     6841 gaatcagtaa ctcggataga caaagctgta ttcgccggtt ctcattcggt ggacaatttg
     6901 atcagcaggc atgactgatc cgatctcagc tttttgattg cgaccggcaa gctttcccac
     6961 tctacggctg ggtccatcta tttcccagac gtgccaattc tttgaaattt ttatctgggg
     7021 atccaaaata tcgttcgttc ctctgaaaag aacatccaaa tcaccgatgt cgcgggaatt
     7081 tccgaccttt ttccatattt tccgaatgat ccccggctta agcacagagt gggcgtagaa
     7141 gtccacggca tctgccgtga tttcgttcac tgcaggcagt gcgttgatgg tgtatttcgg
     7201 cttgaaggct ctgattacgc tgctgttgag ttgtgtccgg tcattagcga tgtactggag
     7261 atatttcttg ctctgatcat ccagaggcac ttcgaaaata tcacctattt tcgttacaat
     7321 ccggctcgtc ataagctaca aattctatcc gctttccccg gatttgcgaa gaacttcact
     7381 gtgccaacgg taggcgaata gaatgcgctt attccacgtt ttctgtccct gatcgatgga
     7441 tgccgggatt taacacggtt cggggaagta attgatgcca gtgccagatt agttgaacga
     7501 gcgaaaactg acctgcctcg gatcagcgac cggaaaagtg tcacttcaag gggagtccgg
     7561 tgaagcgaat cccggcgccg tgaactttgt agcgcatatt caaaccaatc aaaagcgccg
     7621 tcagcgattc cacgatgcgc gcattctcca acttgccccc attcaacgca cgcgcacccg
     7681 gaatcttgct gaccagttcg gaagccacct gctttgctct ctcatcgtcg ctgcacacga
     7741 gaacgtcgca atcgaccggt tcatctccgg cgagcgaatc cgcgccaaga ttttgcaacg
     7801 ctgcggcaag acctacgccg ggaggtagaa gctctttcgt ttgttccgcc gccgagcctt
     7861 gccagacgcc aagcatgcgc gttgctgcgc cgccaaccgt tgccgctagc ggtaccgtag
     7921 tatcgactac tatcgttccc ggcttccaca cgctcttcaa ttgcttgagc aacgccacta
     7981 caccggagaa gggaaccgtc aacaccgcga catcgcccgc ggccgctgca gaaagattgt
     8041 ccataccttc gatctgcgcg gcgctgccaa tgcgttcgcg caatagtctt gcggtctctt
     8101 gcgcgcgcgc cgcctcgcgt gagccaatca ggatgtgttc cccagccttt gccaagcgat
     8161 acgcaagtcc cgagccttcg gctcccgttc cgcccagaat cgcgatcttt cgcgccatga
     8221 tgattcgctc cgttccggat tttgccccac ggcccgatcg cgcacgcagg tcaactccgt
     8281 ctgtatgcgc ggcttcgctt ttcccgcacc cagtatggta tcttcaaaaa cttcccagat
     8341 gctaacgcca ccactcaccg cgctggaatt ccgcaaagcc ctcggccatt tcaccaccgg
     8401 cgtgactgtg gttaccgtcg agcgcgagcc cggcatgatc cacggcatga ctgccaactc
     8461 gttcacctcg gtttcgctcg atcccatgct gattctcgtt tgtgtcgacc agcgcgcgaa
     8521 gttgcttccc ctgctggaga agaaaaaacg tttcggcgtc agcgtcctca aagcagggca
     8581 ggaggctatt tccgaatatt tcgccaaagg cgagcaaagc gcggaagcgg aagagcgatt
     8641 ggcgattcgc taccgatgga ccccgagcgg agttccggtc atcgagcaaa cgattctgca
     8701 gttgagctgc aacgtcgtcg cgtcgcatgt tgctggggac cacaccatct ttgtcgggga
     8761 agtggaagac gcggaagtgc acgaaggcga gccgctgctt tattttcgcg gtgaataccg
     8821 ccggatcgcg cgacactctt aaagaatctt tttccgtgga gagttcgcgc tggtaagaaa
     8881 tgtttttgta aggaattcgt tatcgatcgg gattgccgga aggccgcgtc gattcggccg
     8941 aatcgcccat ctctttgcga tggctcttca cttcgcgcaa cgagtagatc ggcttgttct
     9001 gcgattcgta gtacgtgcgc gcgatgatct cgcccagcaa tcccatggag aagaactgca
     9061 cgccgctgat gaacagcgcc accgccagca tcatcagcgg tccgtgttcc gccatgatgg
     9121 ctttgtggag gtaaaacttt tcgtaggcca ggaagcacgc gagcagggaa cctagccccg
     9181 ccccggccac gcccagcaac ccgaaaaatt ggaggggccg cgtcgagtaa tccagtaaaa
     9241 acttcacgat aatcaaatcc aggaacacgc ggatggtgcg cccgatcccg tagtgggatt
     9301 ttccactctt ccgctcgaga ttctcgatgg gcacttccgc gatttttgcg cccgtcgaac
     9361 ttgccagcgc cggaataaac cggtgcagct cgccgtagag ctgaatttcc tgaatgatct
     9421 cccggcggta ggccttgaac gtggtcccaa aatcgtgcag ttcgattccc gaaagtttcg
     9481 ccatcatcca gttggcggtg cggctgggaa gctgccgcat cagccagtga tctcggcggt
     9541 tgtggcgcca gccactcacc aggtcgtagc ct
//
LOCUS       DASYVO010000276         4645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_35334, whole genome shotgun sequence.
ACCESSION   DASYVO010000276 DASYVO010000000
VERSION     DASYVO010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35334"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1502)
                     /gene="boxB"
                     /locus_tag="VGR03_09140"
     CDS             complement(78..1502)
                     /gene="boxB"
                     /locus_tag="VGR03_09140"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: protein motif:HMM:TIGR03225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEV1994484.1"
                     /translation="MIDYTDKIPNNIDLGRDRVLQRALEHWQPAYLSWWYDMGPNESH
                     GYEVYLRTAISVEPDGWAQFGYVRMPEYRWGIFLQPRDEKRQVNFGTHKGDAAWQDVP
                     GEYRSILRRVIVTQGDTEPASVEQQRMLGLSCPSMYDLRNIFQVNVEEGRHLWAMVYL
                     LHRYFGRDGREEAEALLARRSGDTDNPRILGAFNEPTPDWLAFYMFTFFTDRDGKFQL
                     CALAESGFDPLARTCRFMLTEEAHHMFVGETGVSRIIQRTCELMRENKVDDPSDVRAL
                     GVIDLQTIQRYLNFHYSVTLDLYGSDVSSNAANYYTSGLKGRFEETKLDDDHLLSEAV
                     YPVSEVIGDKIVTKNVSALTALNERLRDDYIREIESGVSRWQRVIDKAGIPFRLTLPH
                     KGFHRAIGNFAGHFISTAGEVLTKEVWEKRRPEWLPSPQDHAYVQSLMSGRVVELGKF
                     ANWIAPPPRGINSQPLNFEYVRFN"
     gene            complement(1540..3222)
                     /gene="boxC"
                     /locus_tag="VGR03_09145"
     CDS             complement(1540..3222)
                     /gene="boxC"
                     /locus_tag="VGR03_09145"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HEV1994485.1"
                     /translation="MDVGISQEPLAKIDRVDYLTDPSRYRHWRLSCDGPVATLTMDVA
                     EDGGIRPGYKLKLNSYDLGVDIELHDALQRIRFEHPEVRAVVITSAKSRIFCSGANIY
                     MLGLSSHAWKVNFCKFTNETRNGIEDSSAHSGLKFLAACNGTTAGGGYELALACDEII
                     LVDDRSSAVSLPELPLLGVLPGTGGLTRVTDKRKVRRDHADVFCTNPDGVRGQRAKDW
                     RLVDEVVKPQQFAEHVKQRALKLTEQSDRPSAAKGVALTPLKRAVDDAGLHYEYVDVR
                     LNREARTVTLTVRAPESVLEDTVEKVLAAGASWWPLQMARELDDAILSLRTSELDLGL
                     WIVHTAGNPGAVLAIDEFILNHQDHWFVREVLGMMRRTFARLDVTSRSLYAIVAPGSC
                     FAGTLLELALAADRIYMRDTQEGESIATMTLSRMNFGSLPMVNHLSRLASRFYQDAGQ
                     IEALRGRMGTKLSASDALAAGLVTAAPDDLDWEDEIRQAIESRAALSPDALTGLEANL
                     RFGVTETLETRVFGRLSAWQNWIFVRPNAVGANGALKVYGTGAPVKFDRERV"
     gene            complement(3232..4137)
                     /locus_tag="VGR03_09150"
     CDS             complement(3232..4137)
                     /locus_tag="VGR03_09150"
                     /inference="COORDINATES: protein motif:HMM:NF006015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1994486.1"
                     /translation="MPASADTRAAVSHRVSHTPEGDFLLLLGKRVREVRNRRGMTRKT
                     VAREAEVSERHLAQLEAGDGNVSIVLLRRIAAALSVSLVELFVPEAEEPVEKQLIQRF
                     LERLPVHRLEDVVSRLMRDFGPEEKVRRMRVALIGLRGAGKSTLGARLSQETNTPFIE
                     LDREIEKDTGMPLGEIFSLYGQSGYRAIERRTLERVLHAYDRAVLSVGGGVVSEKETY
                     DYLLSNCYTVWIKAQPEEHMSRVIAQGDLRAMAGSNQAMEDLRRILEAREPHYRKADM
                     SLDTSGDSVEDSFAKLKRALRANIQ"
     gene            4440..>4645
                     /locus_tag="VGR03_09155"
     CDS             4440..>4645
                     /locus_tag="VGR03_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994487.1"
                     /translation="MKLANYVSGEWKEGAGAGEPLVDPVTGEELGRISSRDLDVRAAL
                     EFARSQGGAALRQSTYRDRAELLT"
BASE COUNT          925 a         1453 c         1348 g          919 t
ORIGIN      
        1 gagggtgcga gcagtgcgga ccgcggccgc gctccaccgc cgaaaactgt cgagggagtc
       61 cagacgccgc ttcccgatca gttgaaacga acgtattcaa agttcaacgg ttgactgttg
      121 atcccgcggg gcggcggcgc aatccaattc gcaaacttgc ccagctcgac gacgcgtcca
      181 ctcatcagcg attggacata ggcatgatcc tggggactcg gcagccactc cggccggcgt
      241 ttctcccaca cctctttggt caagacttct cccgcagtgc tgataaagtg tcccgcgaaa
      301 ttgccgatgg cgcggtggaa acctttgtgc ggcaaggtca ggcggaaggg aatgccagcc
      361 ttatcgatca cccgttgcca gcggctgacc ccggactcga tctcgcggat gtaatcgtcg
      421 cgcaaccgct cgttcagcgc cgtcaacgcg gacacgttct tggtaacaat cttgtctccg
      481 atgacctcgg aaaccggata cacggcttcg ctcagcaggt gatcgtcatc gagcttggtt
      541 tcctcaaaac gccctttgag gccgctggtg tagtaattcg cggcgttcga agatacatcc
      601 gagccataaa ggtcaagcgt aacgctgtag tgaaagttca agtatctctg gatggtttga
      661 aggtcgatca ctcccagcgc gcgcacatcg gacgggtcat cgactttgtt ttccctcatc
      721 aattcgcagg tgcgctgaat gatgcgcgac actcccgtct ccccgacgaa catgtggtgc
      781 gcttcctcgg tgagcatgaa ccggcaggtc cgcgccaggg gatcgaaacc cgattccgcc
      841 agggcacaga gctggaattt gccatcgcgg tcggtgaaga acgtgaacat atagaacgcc
      901 agccagtccg gcgtcggctc gttgaacgcg ccgagaatgc gcgggttgtc ggtatcgccg
      961 gatcgccgcg cgagcagcgc ttcggcctcc tcgcggccat cgcgtccaaa gtagcggtgc
     1021 agcagataga ccatggccca caggtggcgc ccctcttcga cgttcacctg gaaaatgttg
     1081 cgcagatcgt acatcgaagg gcacgatagc cccagcatgc gctgctgttc caccgaggcg
     1141 ggttccgtgt ctccttgcgt aacgatcacg cgccgcagga tcgaccggta ttccccggga
     1201 acgtcctgcc aggccgcatc gcccttgtga gtaccgaaat ttacctggcg tttttcgtcg
     1261 cgcggctgca ggaagattcc ccagcggtac tccggcatgc gcacgtagcc gaattgagcc
     1321 cagccgtcgg gttccacgct gatcgccgtg cgcaggtaga cttcataacc gtggctctca
     1381 ttcggcccca tgtcgtacca ccagctcagg tacgccggct gccaatgctc gagcgcgcgc
     1441 tgaagaacgc ggtctcggcc caagtcgatg ttgttcggaa ttttgtcggt gtagtcaatc
     1501 atcgccatct cctcgacaga tccggccggg agctgtccct caaactcttt cccggtcaaa
     1561 tttcaccggc gcgccggttc catacacttt gagcgccccg ttcgcgccaa cagcgttggg
     1621 acgaacgaaa atccagttct gccatgccga aaggcggccg aaaactcgcg tctccagtgt
     1681 ctcggtgact ccaaagcgga gattcgcctc gagtccagtg agagcatcgg gcgaaagagc
     1741 agcgcgagac tcgatggctt gacggatttc atcctcccaa tcgaggtcat ccggtgcggc
     1801 ggtgacaagt cccgccgcga gagcgtcact ggcagaaagt tttgtcccca tccggccgcg
     1861 cagcgcttcg atttgcccgg catcctggta gaaccgcgac gccagacgcg aaagatgatt
     1921 caccatgggc agcgatccaa agttcattct cgaaagcgtc atcgtggcga tgctttcgcc
     1981 ctcctgcgtg tcgcgcatgt agatgcggtc cgcggcgagc gccaattcca gcagcgtacc
     2041 ggcgaagcag gaaccgggcg cgacaatcgc atacagcgac cgcgaggtca cgtccaagcg
     2101 cgcgaaggtc cgccgcatca tccccagaac ttcgcgaacg aaccagtggt cctgatgatt
     2161 caagatgaac tcgtcgatgg ccaggacggc gccgggattt ccggccgtgt ggacgatcca
     2221 cagacccaga tccagctcgc tggtgcgcag gctgaggatg gcgtcatcca gctcacgcgc
     2281 catctgcagc ggccaccaac tggcgcctgc ggcaagaacc ttttccaccg tgtcctcgag
     2341 cacggactcc ggcgcgcgga ccgtcagcgt gacggtgcga gcctcgcgat tcagccgcac
     2401 gtcgacgtat tcataatgga gtcccgcgtc atcgaccgcg cgcttcaacg gcgtcagagc
     2461 gactcccttc gcggcggagg gccgatcgct ctgctcggta agtttcaacg cgcgctgttt
     2521 gacgtgctcg gcaaactgct gtggcttgac cacctcatca accagccgcc agtccttggc
     2581 cctctgcccc cggacgccgt ccggattcgt acagaagacg tcggcgtggt cccggcgaac
     2641 cttgcgcttg tcggtgaccc gcgtcagccc gcccgtgccc ggcaggacgc ccagcagcgg
     2701 aagctccggc aagctcaccg cggaagagcg gtcgtccacc aggatgattt catcgcaggc
     2761 cagcgccagc tcgtagcccc cgccggccgt cgttccattg caggcggcca ggaatttgag
     2821 gccggagtgg gcgctggagt cttcgatccc attccgcgtt tcgttggtga atttgcagaa
     2881 gttcactttc cacgcatgcg aagaaagacc gagcatgtag atgttggccc cggagcaaaa
     2941 aatacggctc ttcgcgctgg tgatgaccac cgcacgaacc tccggatgct caaaacgaat
     3001 tcgctgcagc gcgtcgtgca gctcgatgtc caccccgaga tcgtaggaat tcagtttcag
     3061 cttgtagccc ggccggattc cgccgtcttc ggccacatcc atggtcaacg tggcaaccgg
     3121 accgtcgcag gagagccgcc aatgccggta gcgggaggga tcggtcaggt agtctacgcg
     3181 gtcgatcttt gccaggggct cttgactgat gccaacgtcc acaagatcct cctactgaat
     3241 attcgcccgc aaagcccgct tcaatttggc aaagctgtct tccacggaat cgcctgaggt
     3301 gtcgagagac atgtccgctt ttcgatagtg cggctctcga gcctcgagaa tccggcgcaa
     3361 gtcttccatg gcctgattgc tcccggccat ggcgcgcaaa tcgccttgcg cgatcacacg
     3421 cgacatgtgc tcctcgggct gcgccttgat ccagacggtg tagcagttgg aaagaagata
     3481 gtcataggtc tccttttccg acaccacgcc gcctcccacg gagaggaccg cgcgatcata
     3541 tgcatgaagc acgcgttcca gcgtgcgcct ttcgatggcc cggtaaccag actgcccata
     3601 cagcgagaag atttctccca gcggcattcc cgtatccttc tcaatctcgc gatccagctc
     3661 gatgaaggga gtatttgttt cctgagacag cctggccccc agcgtcgact tgccggctcc
     3721 acgcagcccg atcagcgcga cgcgcattcg ccgcactttt tcttccggac cgaagtcgcg
     3781 catgaggcgg gacaccacat cttcgagacg atgaacggga agacgttcga ggaaacgctg
     3841 gatcagttgc ttctcgaccg gctcctcagc ttccggcacg aacaactcga ccagagatac
     3901 gctgagggcc gcggcgatcc tcctcagcag tacgatcgac acgttaccat cgccagcctc
     3961 gagctgcgca agatggcgct ccgacacctc cgcttcgcgc gccacggtct tgcgcgtcat
     4021 cccgcggcga ttgcgcactt cgcgaacgcg cttgccgagg agaagcaaga agtcgccctc
     4081 cggagtatgc gagactcgat gggagacagc ggctcttgta tcagcgcttg ctggcatgag
     4141 ggttccgacc cagattcaag ctgcagtata atacatgtga aggccaatcg gtcaacgata
     4201 agcgcgaggt tgcagttcac ctctaaaggg tcacaacaac atccgtctac agaatcccgg
     4261 gataagtcct gctacttgtt tgagatgcat tttgatcctt agacctaaga aaactctgga
     4321 tttgtcgtcc aggaagatgc agtattgtgc cgcattttcg tctggcgtat cccctcgatt
     4381 tgggtacggg gatggcgcta tgctcccgga gtcgtgggct ttcagcggag gaaatctaga
     4441 tgaagctggc taactatgta tctggggagt ggaaagaagg cgctggcgcc ggcgagccac
     4501 tcgtcgatcc cgtaaccgga gaagaattgg ggcgcatttc ttcgcgcgac cttgatgtgc
     4561 gcgcagctct cgagtttgcg cgttcccagg gcggggcggc actccggcaa tccacctacc
     4621 gcgaccgggc cgaactgctg accaa
//
LOCUS       DASYVO010000277         1577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_120384, whole genome shotgun sequence.
ACCESSION   DASYVO010000277 DASYVO010000000
VERSION     DASYVO010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120384"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="VGR03_09160"
     CDS             <1..1040
                     /locus_tag="VGR03_09160"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV1994488.1"
                     /translation="PFDFGYRRATVDAAKADQNRATAETGVTRLGVAVAAANAFMTVL
                     AAQERVKAARANVDRRQVFSKSVHVLTDNQLRPGADASRADAEVAHARTELIRAEQAG
                     RVSRAVLSDVLGIAGTQVEIEAGPLVNLPPATEFPTQAVSAHPVAAAEKARVDEARAR
                     EHIFDRSYFPKFDFQSTVFGRGSGANRDGTVDGGLNGLGLERANWAAGLTVTFRAFDI
                     FSIRAHKEIEKSNERAEAARYDQTIQDLTGQLEQARAALEGARRVLENTPIELHAARD
                     TETQVRARFQAGLATLSDVAEAQSLLVQAETDDALARLTVWNNLASLASAQGELQPFL
                     QMLRTTTQGGP"
     gene            1044..>1577
                     /locus_tag="VGR03_09165"
     CDS             1044..>1577
                     /locus_tag="VGR03_09165"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV1994489.1"
                     /translation="MWLIYAALRRPITVVVAVLSVALCSILAIMRMPVDIFPNLNLPV
                     IYVAQPYGGMDPAQMEGFLVYYYEYHFLYITGIEHVESKSIQNVGLIKLTFHPGTDMS
                     QALAQTISYVDRARAFMPYGTVPPFVMRFDAGSVPVGYLVFSSETRSVGEIQDLALNR
                     VRPLFATLPGVSAPPPFG"
BASE COUNT          312 a          475 c          475 g          315 t
ORIGIN      
        1 aaccgtttga tttcggatat cgccgcgcca cggttgatgc ggccaaggcc gatcagaatc
       61 gggcgacggc ggagaccggt gtaacccgcc tgggagtggc cgtggccgcc gcgaacgcct
      121 tcatgaccgt gctggcggct caagagagag taaaggccgc ccgtgccaat gtggaccgac
      181 gacaggtatt ttcgaaatcg gtgcacgtgc ttacggacaa ccagttgcgc ccaggcgccg
      241 acgcttctag ggccgacgcg gaggtcgccc acgctcgaac cgaactcatt cgcgccgaac
      301 aagccggccg cgtcagccga gcggtgctgt ccgacgttct gggaatcgcg ggcacccagg
      361 ttgagattga ggctgggccg ctagtcaacc ttcccccagc taccgagttt cctacgcagg
      421 ccgtctccgc ccacccggtc gcggccgccg aaaaagcaag ggtggatgaa gcgcgcgccc
      481 gagagcacat cttcgaccgc tcttactttc ccaagtttga ctttcagtcc acggttttcg
      541 gcaggggcag cggcgccaac agggacggga cggtggacgg tggattaaat gggttgggtt
      601 tggagcgagc caactgggct gcgggattga cggtcacctt tcgcgcgttc gatatctttt
      661 ccatccgcgc tcacaaagaa atcgaaaagt ctaacgagcg cgctgaagcg gctcgatacg
      721 accagacaat tcaagatttg accgggcaat tagagcaagc gcgagcggcg ctggaaggtg
      781 cgcgacgcgt cctcgaaaat actcctatcg agctacacgc cgcgcgtgat acggagactc
      841 aggtccgcgc ccgctttcaa gcgggactcg ccaccctttc cgatgttgcc gaagcgcaga
      901 gcctcctggt tcaggcagag accgatgacg cgctggctcg gctaactgta tggaataacc
      961 tggcgtcact ggcatcggcg cagggcgagc tgcagccatt cctgcagatg ttgcgaacga
     1021 caacccaggg agggccataa acaatgtggc tgatctatgc cgcattgcgg cggccgataa
     1081 ccgtcgtggt tgccgtcctc agcgtcgccc tatgctccat cctggcgatc atgagaatgc
     1141 ccgtcgacat ttttcccaac cttaacctgc cggtcattta tgtggcccag ccctatggcg
     1201 ggatggaccc ggctcaaatg gaagggttcc tggtttacta ttacgaatac catttcctct
     1261 acatcaccgg aatagagcac gtcgaatcga agtccattca gaacgttggc ctcatcaagc
     1321 ttacgtttca tccgggcacg gacatgagcc aagctctggc gcagacgatt tcttacgtgg
     1381 atcgcgcgcg cgcgttcatg ccttacggaa ccgttccgcc cttcgtgatg cgctttgacg
     1441 ccggcagcgt gcccgtggga tatcttgttt tttcgagcga gacgcgcagt gtgggagaaa
     1501 ttcaagacct ggcgctcaat cgtgtgcgac cgctgtttgc gactctgcct ggcgtctccg
     1561 cgccgccgcc gttcggg
//
LOCUS       DASYVO010000278         3219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_275132, whole genome shotgun sequence.
ACCESSION   DASYVO010000278 DASYVO010000000
VERSION     DASYVO010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275132"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VGR03_09170"
     CDS             <1..835
                     /locus_tag="VGR03_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994490.1"
                     /translation="AGYAWAHSIDDVSLNRAPQPQDSTRPGLERASSDNDVRHRITLA
                     LTYELPSRKGYGHLLEGWQLNSIATIQGGLPWNLIDGNVSGSDSSGTGEFADRWNITG
                     SPANFKPSTSGIPYVDPSLFTVDANPMSPTFGHVIGGTTPASTTCFSVAASQGAKDQL
                     ATLGCYVAGSSALTPPAFGTFGNAGRNPFRGPRLDNLDFSIVKTTNLNERVSFQLRAE
                     FFNAFNHPHFANPNTQGLVDPSDHTTFGFIFNTPDVAAANPVIGTGGPRNIQFGLKFR
                     F"
     gene            complement(916..2163)
                     /locus_tag="VGR03_09175"
     CDS             complement(916..2163)
                     /locus_tag="VGR03_09175"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV1994491.1"
                     /translation="MKIRTLCSCLMALVVFALPSSGQTNPAPQLIAIKAGKIIDPETG
                     KAALNQIILVEGKKIKEIGGNVAIPAEAKLIDLSKATVLPGLFDAHTHLCLSVIPSRD
                     HGDYYFTTLLDTTGYRAIEGVANGMAMLQSGFTTVRDIGNAGNYADTDLRHAIEAGWV
                     QGPTVVNAGIIIAPYGGQFHLQPEKKDLATPEYLFADTRDEMRKAIRQNLHFGAKFIK
                     IVVDDQRYIYSADDIRFMIAEAHAAGVRLAAHCWTHAGAHNAAEAGVDSIEHGFKMTD
                     DDLQLAKKNNVTLVGTEFTEKMSNADEHKIWVDRLRRAYKIGVNMAFGTDVDVALPGE
                     TRGTLAIDYIQSWVDAGVPATDTLRAMTVNAARLLSVDQERGFLKPGMAADIIATPEN
                     PLENIQTLRKVSFVMKDGAIIKQ"
     gene            complement(2197..>3219)
                     /locus_tag="VGR03_09180"
     CDS             complement(2197..>3219)
                     /locus_tag="VGR03_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV1994492.1"
                     /translation="DLGDAEPLLVLQKSAAESAYAVIPNARVTLLAGFTTVRDVGVYR
                     AFNDVALRDAIARGIVIGPRMFVAGAYITISEGAGAMTGLAPDMQLPWDLHYGEANSP
                     WEVRQKIRELAHRGADHIKILSTGAVLTHGSNPKSIEFTPEELQAAVDEARNFGLRVE
                     AHAHAAEGIKNAIRAGVASIEHATLIDDEGIALAKQHGTYLDMDIYDEECIQDAAKEN
                     KTPADFLEHDRDLGETHRRNFTKAVRAGVKMSFGTDAGVCPHGINARQFAFMVKYGMT
                     PMQAIQSATTNAADLLGKSELLGSLQPGKFADIIAVSGDPLENIRLLENVKFVMKEGK
                     IYKQEP"
BASE COUNT          624 a          945 c          928 g          722 t
ORIGIN      
        1 cgccgggtat gcctgggcgc actcgattga cgatgtatca ctgaatcgcg cgcctcaacc
       61 acaagacagc acgcgcccgg gactggagcg cgccagcagt gacaacgatg ttcgccaccg
      121 tattacgctg gcgctgactt acgaactccc ttcgcgaaag ggttacgggc acttgctcga
      181 aggctggcag ctcaactcca tcgcgacgat tcaaggtggc ttgccgtgga acctcatcga
      241 cgggaacgtc agtgggagcg acagcagtgg gaccggagaa tttgccgacc gctggaacat
      301 caccggcagt ccagcgaatt tcaaaccttc aacttccgga attccatacg tagatcccag
      361 tcttttcacg gttgacgcga atcctatgtc tcccacgttt ggccatgtca tcggcgggac
      421 gactcccgcg tcgacaactt gcttttcggt tgccgcgagc cagggggcca aagaccagct
      481 tgccaccttg ggatgctatg ttgcgggatc ctcggccctc acgccaccgg cgtttggcac
      541 atttggaaac gccgggcgca atcccttccg cgggccgcgg ctggataact tggacttctc
      601 gatcgttaag acgacaaacc tgaatgaacg tgtgagcttc caattgcgcg cggagttctt
      661 caatgccttc aaccatccgc acttcgccaa tccgaacacg caggggctgg tggatccgtc
      721 cgatcacacc acgtttggat tcatcttcaa tactccggac gtggccgcgg caaacccggt
      781 gattggaacg ggcgggccgc ggaacatcca attcgggttg aagttccgtt tctagctgcg
      841 cttctcaatc cagaaacaaa aacaggccgg atcttgcgat ccggcctgtt ttttttaccg
      901 aggattagca caggactatt gcttgatgat cgccccatcc ttcatcacga aagagacttt
      961 tctcaaagtc tgaatgtttt cgagcggatt ttccggcgtt gcaatgatgt ccgcagccat
     1021 cccgggcttc aggaagcccc gttcctggtc cacactaagc agtcgcgcgg cattcaccgt
     1081 cattgcccgc agcgtatctg tggcaggcac tcctgcgtcc acccaacttt ggatgtagtc
     1141 gatcgccagt gtcccgcgcg tttcgcccgg tagtgcgacg tcgacatccg tgccaaaggc
     1201 catgttcacc ccgatcttgt aggcccgccg cagccggtcc acccaaatct tgtgttcgtc
     1261 ggcattgctc atcttctccg tgaattccgt tcccaccagc gtcacattgt tcttcttggc
     1321 aagctgcaaa tcgtcatctg tcatcttaaa gccgtgctcg atggaatcga cgcctgcctc
     1381 cgcggcattg tgcgcgccgg cgtgggtcca gcaatgtgcg gccagtctta cgcccgcggc
     1441 atgggcctcc gcgatcatga agcgaatatc gtctgccgaa tagatgtacc gctgatcgtc
     1501 gaccacaatc ttgataaatt tcgcgccaaa atgaaggttc tgacggatgg ctttgcgcat
     1561 ctcatcgcgc gtatccgcga agagatattc gggcgtcgcc agatctttct tctccggctg
     1621 caggtgaaac tgtcccccat agggcgcgat tatgatgccg gcattcacaa ctgttgggcc
     1681 ctgcacccat cctgcttcaa tcgcgtgacg caggtcggtg tccgcataat ttccggcgtt
     1741 gccgatatcg cgcactgtag tgaagccgga ttgcaacatg gccatgccgt tcgccacgcc
     1801 ctcgatggcg cgatacccgg tggtatccag cagggtggtg aagtagtagt cgccgtggtc
     1861 gcggctgggg atcacggaca agcacagatg cgtgtgcgca tcgaacaggc ccgggaggac
     1921 cgtggctttc gaaagatcga taagctttgc ttccgcgggg attgcgacgt tgccgccaat
     1981 ttccttgatc ttttttcctt ccaccaaaat aatctgattg agggcagcct tacccgtttc
     2041 cgggtcgatt attttccccg ctttgatcgc aataagttgt ggcgcaggat tggtttgccc
     2101 cgaggagggc aacgcaaata cgacaagcgc cataaggcaa gaacacagcg tccgaatctt
     2161 catggactct ccccttccag atagttaggc gcccgcctag ggctcctgct tgtagatctt
     2221 tccctctttc ataacaaact tcacgttttc gagaagacgg atattttcga gaggatcgcc
     2281 ggagactgca atgatgtcgg cgaactttcc aggctggagg gagccgagga gctctgactt
     2341 gccgaggaga tcggcggcat tcgtggtggc ggattggatg gcttgcatag gcgtcatgcc
     2401 gtacttcacc atgaacgcaa actggcgggc gttgatgcca tgcgggcaga cgcctgcgtc
     2461 ggtgccaaag gacattttca cgccagcgcg aacggccttc gtaaagttgc ggcgatgagt
     2521 ctcacccagg tctcggtcgt gctccagaaa atccgcaggc gtcttgtttt ccttggccgc
     2581 gtcctgaatg cattcctcat cgtagatatc catgtcgagg taggtgccgt gttgcttagc
     2641 gagggcgatg ccttcgtcgt caatgagcgt ggcgtgttcg atggaggcaa cgccggcgcg
     2701 gatggcgttc ttgatcccct cggcagcgtg cgcgtgggct tcaacgcgca ggccaaagtt
     2761 gcgagcttcg tccactgcgg cctgcagctc ttcgggcgtg aactcgatcg atttcggatt
     2821 gcttccgtgc gtgagcaccg cgcccgtgga tagaatctta atgtgatcgg cgccgcggtg
     2881 agcgagctca cggattttct gtcgaacctc ccagggacta ttggcttcgc cgtagtgaag
     2941 atcccacggg agctgcatgt ccggcgccag accggtcatg gcgccggcgc cctcgctgat
     3001 cgtgatgtaa gcgccagcga cgaacatgcg cgggccaatc acgatgcctc gggcgatcgc
     3061 atcgcgcaga gcgacatcgt tgaaggcgcg atagacgccg acgtcgcgca cggtagtgaa
     3121 gccggcgaga agtgtgacgc gcgcgttggg aatgaccgcg taggcgcttt cggccgcgga
     3181 tttttgcagc acgaggagcg gctcggcgtc gcccaggtc
//
LOCUS       DASYVO010000279         1506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_261501, whole genome shotgun sequence.
ACCESSION   DASYVO010000279 DASYVO010000000
VERSION     DASYVO010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261501"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VGR03_09185"
     CDS             <1..506
                     /locus_tag="VGR03_09185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEV1994493.1"
                     /translation="SPNWYQVYRAAGLPRRRWEDADRAWRAHYAKHRPKLIAGARRVL
                     ARVGNAHTLGLVTSGDRDRVIRQLRQFRLTTLFAARVCSGDTLRKKPHPEPLRLALRQ
                     MELESSACVYVGDAPQDVEMAQRAGVRAIGVLGPFPTEKRLRAARPEFLIGSIEELPD
                     VLKRLLR"
     gene            complement(533..>1506)
                     /locus_tag="VGR03_09190"
     CDS             complement(533..>1506)
                     /locus_tag="VGR03_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994494.1"
                     /translation="RSALNSCFGSAREAAQRGIARIQFPMSQARLSRCYKHISFVLPA
                     PLVVLILAAMQGSPLRAQDAPQGPIAPPPEHHVTRIGNVSQPEAPPSLPEADIVKRFS
                     QKEDEYMLSRTHYTYRKTIRIQEFGPDGQAAGEFVLVTQPGRDADGKLFEKVIERPRS
                     TLQHLFLRSEDLEGLQRIPAFPLTSGQLAKYDLKYLGKEQVDEIDCYIFQAKPRGVER
                     VKAYFDGIVWVDAKYLEVVKTYGKWVTDQGDVHTMADLPFSLFETYRENVDGKYWFPS
                     YSRSDETLNLKGLEIPVRLVIKWTDFKPVPIAPQVPAPSAPTTSPKS"
BASE COUNT          279 a          427 c          475 g          325 t
ORIGIN      
        1 attcgccgaa ctggtaccag gtttatcgcg ctgcggggtt gccgcggagg cgctgggaag
       61 atgcggaccg cgcgtggcgg gcgcactatg caaagcaccg gccgaagctg atcgcgggcg
      121 cgcgccgggt cctggcgaga gtgggcaacg ctcatactct tggcctggtc accagtggcg
      181 atcgcgatcg tgtcatccgt caattgcgcc aatttcgttt gacgacgcta ttcgcggcgc
      241 gcgtttgcag cggagacacc ctgcgcaaga aaccgcatcc cgagccgctg cgcctggctt
      301 tgcggcagat ggagctggaa tcctccgcgt gcgtctacgt aggcgatgcg ccgcaggatg
      361 tggagatggc ccagcgcgcc ggtgtgcgtg cgattggcgt gctcggcccc ttccccacgg
      421 aaaagcgtct gcgcgcggct cggccggagt ttctgattgg ctcgatcgag gaattgccgg
      481 acgtactgaa gaggttgctc cgctgatgga ccggtttttt tagcggccgt gcttatgatt
      541 tcggagaagt tgtaggagcc gacggcgcgg gtacctgcgg cgcgatggga acgggtttga
      601 agtccgtcca cttgatgacc agacgaacgg gaatctccag ccccttcaga tttagcgtct
      661 catccgagcg ggagtagctc gggaaccagt attttccgtc gacgttttcg cggtaggttt
      721 cgaacaggct gaagggcaga tccgccatcg tgtgtacgtc gccttgatcg gtaacccatt
      781 tcccgtacgt tttcacgacc tccagatatt tcgcatccac ccacacgatg ccgtcgaaat
      841 aggccttgac acgctcgacc cctcttggtt ttgcctgaaa gatgtagcaa tcgatttcat
      901 caacctgttc cttgcccagg tatttcaggt cgtatttcgc aagctggcct gaagtgagcg
      961 gaaacgcggg gatgcgctgc aggccttcga gatcttccga cctgagaaag aggtgctgga
     1021 gcgtggaacg gggcctttcg attaccttct cgaataactt cccatccgca tcccggcccg
     1081 gttgcgtcac tagcacaaat tcccccgcag cctgcccgtc cggaccgaac tcctggatac
     1141 gaatcgtctt tcggtaggta tagtgtgtgc gcgagagcat gtactcgtct tctttttgag
     1201 aaaagcgctt gacgatatcg gcctcgggga gcgacggcgg cgcctccggc tgggatacat
     1261 ttccgatgcg cgtaacgtga tgctccggcg gcggagcgat cggcccctgg ggagcatcct
     1321 gcgcgcgtag cggcgatcct tgcatggcgg ccagaataag tacgacaagg ggggcgggca
     1381 aaacgaatga gatgtgcttg tagcaccgcg atagtctggc ctggctcata ggaaattgga
     1441 tgcgcgcaat tcctctttgg gcagcctcac gcgcgctgcc gaagcaagag tttaacgcac
     1501 tccgag
//
LOCUS       DASYVO010000280         3541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_51541, whole genome shotgun sequence.
ACCESSION   DASYVO010000280 DASYVO010000000
VERSION     DASYVO010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51541"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(620..3268)
                     /gene="glnD"
                     /locus_tag="VGR03_09195"
     CDS             complement(620..3268)
                     /gene="glnD"
                     /locus_tag="VGR03_09195"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEV1994495.1"
                     /translation="MTSAARPGLNLGIPPAKNMQGSPTPLSVLGPQADAERQRIRQQF
                     EAGSGVREALHAQCELADRNVQQIFGEVLRVHNREPQGLCLLALGGYGRRMLFPYSDL
                     DILFLLGNERAEQESRPLISEFSRILWDIGFRVSSAGRTLEECRRIEEDNAEFHLALL
                     DRRFLAGDAALFEKLDTKVLPASEKQARPFLLAQIHRLTRERLTRYGNTIFHLEPNVK
                     ESPGGMRDYQATIWLRQIVDEKKGIRNITAAEEELSAAAVDFLSAIRCFLHYSNGRND
                     NTLTYELQAAAAERALGVGDGVARTPAEWMRQYFRHARTLNRQLLRYLEQKAPVPLTL
                     RQRLFNAARAAKVEPRNGKSFTVRDGLLEVLDQPVLSDRAIAYSLFAEAARTGTPLSR
                     EAERSIGYILTHPELPPKNTKISWDTLREILGSDYPGVALRSMQRLGLLAEILPEFGT
                     IDSLVVRDFYHRYTVDEHSLRTIEHLQELAEPPDERGAHFAPLWKTAERRDLLILSLL
                     LHDVGKGLPVENHVTGSLAALDSAAPRLNLSPEEKEEVHFLIERHLEMSATVQRRDIF
                     DPATVSAFAQSVTTLERLQRLCLLTYADIHSVNPEALTPWKAEMLWQLFVATSNHFSR
                     TLDRDRLHASDEGSLLERVKALASDASLEEIERFFEGFPRRYLAVHSAAEIAAHFALY
                     RKLGTDPVQTELLATRHGFSLTLLTADRPALFATISGALAGWGMNINKADAFANAAGV
                     VLDTFHFADLHRTLELNPSESKRFRKSLEDVVNGKAPLEPLLKSRESASRGRVPKVTV
                     ETRINFDDASSAHSTLLEIVTQDHPGLLYEIGSALARLGCNIEVALIDTEGHKAIDVF
                     YLTAQGKKLTTQKQELLREVLQGSLV"
     gene            complement(3322..>3541)
                     /locus_tag="VGR03_09200"
     CDS             complement(3322..>3541)
                     /locus_tag="VGR03_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEV1994496.1"
                     /translation="GHTEVYRGREYTVDLLPKIKLETVLPDALADRAVETILKAAKTG
                     KIGDGKIFLSKVDEAIRIRNEERGENAL"
BASE COUNT          653 a         1079 c         1041 g          768 t
ORIGIN      
        1 gagtggggcg gccaagtggc aaccctcccc ccgtgatttc gtccaaaccc tggattccgg
       61 tggctgcttt cggcagcgtt ttggaagatg gctggcttgc tttcttcttt tgtcccattt
      121 gtttcatcct ggccgcgggc gatctacgcg ggttggcagt gccggagcca acgccccgcc
      181 tcgtgcggcg ccgttaccaa acggaggcca ctcggttcct tcaccataca ctgcttctct
      241 agaaaaacag ctatagtttc taccaggata gagtgagagc tagttagatc agttgcatac
      301 tcgctgcgga atgtgcccaa ttttgtgccc accaccaatc cctaccgagt gctaccgggt
      361 gtcggacccg caacgctaag cgcctgcgat cgttgcactt gtagcaatcg gtagcagttc
      421 ctcgcacgca gtgtaagttg ttgattcttc gtgcgaccaa tctttgcaag cagggggtcg
      481 ccggttcgat cccggccacg tccaccattt ctcaggttca aagctgccgc aattcgtcca
      541 agtttggcgt ccccgtccca cccgtgtttt tcccttccga catctcttgc aggaatggga
      601 tttaatgtac agggacgggc taaaccagac ttccttggag cacttcgcgg aggagctcct
      661 gcttttgcgt ggtgagcttc ttgccttgcg cggtaaggta gaacacatcg atggccttgt
      721 gaccttccgt gtcgatcaat gcgacttcga tgttgcatcc caggcgcgcc agcgccgaac
      781 cgatctcgta gagcaatccc ggatgatcct gcgtaacgat ttcgagcagc gtgctgtgcg
      841 cggatgacgc atcgtcgaag ttgatgcgcg tttcgacggt gactttcggc acgcgccctc
      901 ggctggcgct ctcgcggctt ttcaaaagcg gctccagcgg cgcctttccg ttcacgacgt
      961 cctcaaggct cttgcggaaa cgtttggatt cgctggggtt cagctccagc gtgcggtgca
     1021 gatccgcgaa gtggaaggtg tccaacacaa cgccggcggc attcgcaaag gcatccgctt
     1081 tattgatgtt catgccccag ccggccagcg cgcccgaaat cgtcgcgaag agcgcggggc
     1141 ggtccgctgt gagcagcgtc agggagaacc catggcgggt ggcgaggagt tccgtctgca
     1201 cgggatcggt gcccagcttg cggtagagcg caaagtgtgc cgcaatctcc gccgccgaat
     1261 gcaccgcgag gtaccgccga gggaatccct cgaagaaccg ctcgatctct tcgagggagg
     1321 cgtcgctcgc cagagctttc acgcgctcga gcagcgagcc ttcatccgat gcgtgcaggc
     1381 ggtcgcgatc cagcgtgcgg ctgaagtggt ttgaagtggc cacgaaaagc tgccacagca
     1441 tttctgcctt ccacggcgtc agtgcttccg ggtttacaga gtgaatgtcc gcataggtca
     1501 gcaggcacaa tctttgcaga cgttccagcg ttgtgacgga ctgcgcaaaa gcagaaacgg
     1561 tggcaggatc aaagatgtct ctccgttgca cggtggcgga catttccagg tggcgttcga
     1621 tgaggaagtg gacttcttct ttttcttccg gcgaaagatt caatcgcgga gcggcgctat
     1681 cgagcgccgc gagactgccg gtgacgtgat tctccaccgg cagtcctttg cccacgtcgt
     1741 gcagcaacag cgacaggatc agcaaatccc gccgctcggc agtcttccag agcggcgcga
     1801 aatgcgctcc tcgctcgtct ggtggctccg ctaattcctg caggtgctcg atggtccgca
     1861 gcgaatgttc gtccaccgtg tagcgatgat aaaaatcgcg caccaccagc gagtcgatcg
     1921 tcccgaattc cggcaggatt tccgccagca gccccagccg ttgcatcgac cgcaaagcca
     1981 cgcccggata atcggagccc agaatctcgc gcagcgtgtc ccaggaaatt ttcgtattct
     2041 tcggagggag ttccggatgc gtcaggatat aaccgatgct gcgttctgct tcacggctca
     2101 gcggagtacc cgtgcgagcc gcttccgcga agagcgagta ggcgatcgcg cggtccgaca
     2161 gcaccggctg gtcgagcacc tcgagaaggc cgtcacgcac cgtgaaagat ttgccattcc
     2221 tcggctcgac cttggcggcg cgtgccgcat tgaacaagcg ctgccgcagc gttaacggca
     2281 cgggagcttt ctgctcgaga tagcgcaaga gttgccggtt cagcgtgcgg gcgtgccgaa
     2341 aatactggcg catccattcg gcaggagttc gcgccactcc gtcgcccaca cccagcgcgc
     2401 gttctgctgc cgctgcctgc agctcatagg tgagcgtgtt gtcgtttcgg ccgttgctgt
     2461 agtgcagaaa acatcggatg gcgctgagaa aatcgacggc cgcggccgaa agctcttcct
     2521 ccgcggcggt gatgtttcga attccctttt tttcgtcgac gatctgccgc agccagatgg
     2581 tggcctgata atcccgcatg ccgcccggcg actctttcac gttgggctcg aggtggaaaa
     2641 tcgtatttcc gtagcgcgtc aatcgctccc tggtcagcct gtgaatttgc gccagcagga
     2701 aaggccgcgc ctgcttttcc gaggccggga gcactttcgt gtccagcttt tcgaaaagcg
     2761 cggcatcgcc cgccaggaat cggcgatcca gcaatgccag gtggaactcc gcattgtcct
     2821 cttcgatccg cctgcattct tccagcgtgc gtccggccga actgacccgg aatccgatgt
     2881 cccacaaaat tcgcgaaaac tcggagatga gcggccgcga ttcctgctct gccctttcgt
     2941 ttcccagcaa aaacagaatg tcgagatcgg agtacgggaa cagcatgcgc cgtccgtacc
     3001 cgcccagcgc gagtaggcac agcccttgcg gctcgcgatt gtggacgcgt agaacttcgc
     3061 caaaaatttg ttggacgtta cgatcggcta gttcgcactg cgcgtgcagc gcttctcgca
     3121 caccgctccc ggcctcaaac tgctgccgga tgcgttgccg ttcggcgtcg gcctgcgggc
     3181 cgagcacgga taagggcgtt ggggaacctt gcatgttctt tgcgggcgga atgcccagat
     3241 tgagaccggg gcgtgcagcg ctcgtcacgt tgatcgcccc ctggtcagta agccggccgc
     3301 ccggcggcca agagtgaaag gctaaagcgc gttttcaccg cgctcctcgt tacgaattcg
     3361 gatagcttca tcaaccttcg ataggaatat cttaccgtcg ccaatcttcc ctgtcttggc
     3421 cgccttcaaa attgtttcta cggcgcgatc cgccagggca tccggcagca ccgtctccag
     3481 cttgatcttt ggcagaaggt ccacggtgta ttcccgcccg cggtagacct cggtgtggcc
     3541 c
//
LOCUS       DASYVO010000281        22707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_92283, whole genome shotgun sequence.
ACCESSION   DASYVO010000281 DASYVO010000000
VERSION     DASYVO010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 22707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22707
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92283"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            439..2850
                     /gene="gyrB"
                     /locus_tag="VGR03_09205"
     CDS             439..2850
                     /gene="gyrB"
                     /locus_tag="VGR03_09205"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV1994497.1"
                     /translation="MGDKENVGGAPQGGHKYDAKSIKVLGGLEAVRLRPAMYIGSTGE
                     MGLHHLVWEVVDNSVDEAMAGYADEINVTVHADESVTVVDNGRGIPVDMHATEKRPAA
                     EVVLTVLHAGGKFDNDTYKVSGGLHGVGVSVVNALSDRLELEISRDGAVWEQTYEKGK
                     PTNKLKQTGKTRKTGTKVTFHPDPSIFDKTVYSFDTLAQRLRELAFLNKGLKITLTDE
                     RTEKTTEFKFSGGITEFVKHLNRGKTTLHDSPIYIEGKRGNVEIEIALQYNDTYGENV
                     FAFANTINTVDGGTHLSGFRSSLTRTINTFASGAGMLKEQKDEVTITGDDVREGLVAV
                     VSVRLPQPQFEGQTKGKLNSDIKGDVEQLVNERLGDWFDKHSTVARRVIGKCIEAARA
                     REAARKARELTRRKSAMDSSGLPGKLADCQERDPARCELFVVEGESAGGSAKMGRDRK
                     YQAILPLKGKILNVEKARYDKMLGHEEIRAIITALGTGIGKDDFDAGKLRYHKIILMT
                     DADVDGSHIRTLLLTFFFRHMKELIERGHIYIAQPPLYRVKRGKTEKYIRDERDFARE
                     LMKRATEDHVVKAKEGVNLEGARLTSFLLNVQEYEAAAAKLGRRLREHGLVELLAESG
                     LEKKTDFEDKKALEELLKQLEKTKLKLEGKIVFDEEHSLYEIQFGPPHSQKINWAVAS
                     TAEYKRLRGLAKQIEEFNHPPFTVARNGDKVTKEKATDVLNYVVEDAKKDFTITRFKG
                     LGEMNAEQLWDTTMNADTRTLLQVKLEDAVAAEDIFTTLMGENVEARRKFIEENALEV
                     VNLDI"
     gene            2861..3109
                     /locus_tag="VGR03_09210"
     CDS             2861..3109
                     /locus_tag="VGR03_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994498.1"
                     /translation="MLYQLGWATLPGLRGLSCSGFRATPTSAPDNERGVAIEFAGNDE
                     RDAFLRQIEEHFAERRFTNSADAFDTVKAYVLEHAAKR"
     gene            3177..5840
                     /gene="gyrA"
                     /locus_tag="VGR03_09215"
     CDS             3177..5840
                     /gene="gyrA"
                     /locus_tag="VGR03_09215"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV1994499.1"
                     /translation="MADEINTSVLLPIDIEVEMRKSYLDYAMSVIIARALPDVRDGLK
                     PVQRRILVAMNDLGLASNRGYRKCAKICGDTSGNYHPHGEAVIYPAMVRLAQDFNLRY
                     MLVDGQGNFGSVDGDPPAAMRYTEARLAKISEDILADLEKETVDFIPNFDQTREEPVV
                     LPSRIPNLLVNGASGIAVGMATNIPPHNLREIVDAAILLIEKPDTPLKEIMKIVPGPD
                     FPTGAYIAGREGIEAAYRMGRGSFTMRAKAAIEETTKDRESIIITEIPYQVNKARLIE
                     RIAELVQTKKLEGISDVRDESSREGMRVVIELKRGEESQLILNNLFKFTQMQESFGMI
                     LLSIVAGQPRELGLIAMIKLFIEHRIDVIRRRTIFELKKAEQREHILLGYQIALDHLD
                     NVIRIIRGSSSRVEARENLFKYFSEQDVTITENGKPKKIEGVKLDGRKYRAQGAPEIE
                     AAGHGNALGLSYAQIDAILELQLHRLTQLSIDEIVKELKSIRELIAELREILASDKKL
                     KQVIVAELREVHKAYGDDRRTQIVDKVDEIKLEDLIADTEMLITVSHAGYIKRTAADT
                     YRHQSRGGKGRIGARTKEEDFVEHLFIASAHSYILLFTSKGRVYWLKVYELPEAAAAT
                     RGKAISSLVKFQEDEKLTALVAARNLEEAGQFVFMATKSGTVKKSALTEFSNPRSTGI
                     IALTLDGKDELIGAKLTDGKKMIFLASHEGQAILFRETEVRPMGRNAGGVNGMDLDKE
                     DYVVSMDAVQPDFEIIAKEHATTTQNLEELENEQIKDSLVTLMLTVSEKGFGKRTPLA
                     EYRITSRGGKGVVNMKTTDRNGYVVATLQVTEESDVMIIAHNGKVIRVHSNEIREAGR
                     STQGVRLLRLEDDDSVAAAAVLQEENEEEKK"
     gene            5921..7126
                     /locus_tag="VGR03_09220"
     CDS             5921..7126
                     /locus_tag="VGR03_09220"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV1994500.1"
                     /translation="MKITRRIFTFSLLIAPLVFSVAGESADKPAANRKYLVFVGTYTH
                     KTESKGIYGYEFDADTGKLTPKGVAVETLDPSWIAVHPSGKFVYAANEAGKASTVSAF
                     VVDGKSGKLTLLNQKPALGEDPCYLSFDKTGKYILVANYSSGTIAVFPILADGRLGEH
                     TALVKDQGATGPNKKRQEAPHAHWIETTAHNHFVYVADLGLDRVLIYKFDATKGSLTP
                     GEPLPSKATPDKEAPLDPFSVTLNPGAGPRHVAFARDGKFMYVLGELQSTVTVFANEA
                     QETYRSVQQISTLPKGFAGRNDAAEIAVHPNGKFLYASNRGHDSIAIFAIDPAKGTLT
                     FISHVLTGGKEPRHFAIDPSGKYLLAENQFSNNVVVFKIDPATGGLTPTGQVVEVPSP
                     VDIAFLQED"
     gene            7582..7962
                     /locus_tag="VGR03_09225"
     CDS             7582..7962
                     /locus_tag="VGR03_09225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEV1994501.1"
                     /translation="MAKRKARKVGARKPKRSTRRAHGAAGGKLPKEAVTLIVILRARE
                     GQETLLEAELRALVGPSRKEDGCLTYSIHRSIDTPGALLLHEVWASREAHTEHTHTPH
                     FLRWNARKDALLASRDANFWKQIV"
     gene            7994..8371
                     /locus_tag="VGR03_09230"
     CDS             7994..8371
                     /locus_tag="VGR03_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994502.1"
                     /translation="MSYDTKILILVFAGSFPLAVLYLYLKDFLYTKTTGRPASQRYRE
                     RVVRWYGPLTRRKYIAFCIFLGLTTLLSAYSVGWSGEYRGWSKLSPLIRMIFAAFLLF
                     FLQKRWRKQQAGLNDQPSGAARK"
     gene            complement(8344..9498)
                     /locus_tag="VGR03_09235"
     CDS             complement(8344..9498)
                     /locus_tag="VGR03_09235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV1994503.1"
                     /translation="MIRIAGASLTLTLLLALADAPANAQAPVGPLPPKPGVEVQLPPT
                     NTQARVKVQVTLVNTPVTVRTGRGDMVHNLEARDFQITDNGVAQQISHFDLGGDPLSL
                     VILLQTSSRIEPLLPQVRKTGILFTQTVMGSTGEAAVVSFNDGVDKLQDFTANGDLIE
                     NTIAHLGTGTSGSKLYDAMAVGVEMLSGRPQATADKPGRRRVLMILSEAADAGSEAKL
                     GEVLRQAQLANVTIYSVGLSTTRAELQAKPKDTRPKITPPGTFPLPPQPGTPQTPTSE
                     ENRYGNIDLMALAVWAVQHVHDQVKDHALEVAATATGGTHLATFRDRSIEKAIDEIGG
                     ELHSQYTISYTPTGSDAAGYHEIKVNVLRNDAKNLKVRARPGYFLAAPEG"
     gene            9672..10547
                     /locus_tag="VGR03_09240"
     CDS             9672..10547
                     /locus_tag="VGR03_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEV1994504.1"
                     /translation="MSDGKKLKPQHAPKAPHEDPEFLNSTPARPIRILGEYIHPLVQL
                     KREGIVDTIVMFGSARIESHETAQARCTRLKNEKTSKMSAANRAKHRADVRHAKSLLE
                     MSRYYEEARQLSHKLTTWSLTLGPRPRRFVVCSGGGPGIMEAANRGAYEAGGKSVGLS
                     IELPHEQFANPYISPELSFNFKYFFMRKLWFAQIAKALIVFPGGFGTMDELWEMLTLL
                     QTGKLAKHNIILIYGRKYWDEILNWKAMVRWGTINEDEYKMLQFADNVDEAFDHIRAG
                     LEKYHMAVDPFLQAY"
     gene            complement(10617..10931)
                     /locus_tag="VGR03_09245"
     CDS             complement(10617..10931)
                     /locus_tag="VGR03_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994505.1"
                     /translation="MDRHNLEAVGVCRTGWAALDRDPQQDTVEFICPHCGARDKKNAR
                     RERALYHFTDGFLQDLQGEVTQCHACKQYLRVVPIVMLHDQDETRRFEAVFSDEQLVT
                     SH"
     gene            11288..12079
                     /locus_tag="VGR03_09250"
     CDS             11288..12079
                     /locus_tag="VGR03_09250"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEV1994506.1"
                     /translation="MSPVPKTVSGEIAEASLGSLSGCLVDGDAEQRSRERSIRRRSLA
                     ISILLQSAVLATLILVPLFGKAERISLTIVTPIPPYSPYRDVSQNPGEQRPRPHGPQN
                     VCRFCPPLRIPLTIAMRDQASSTSETDETPFEGQGIPGAIDGLIPLSDSRQRVLPPPN
                     TERQGTRPQLVHVGTINPAMLIHRVEPVYPALARQTHREGRVELRAIISTDGSIQSLQ
                     VVSGDALFLISAREAVQQWRYRPTILDGQPVEVDTYITVIYTTQH"
     gene            complement(12175..12741)
                     /locus_tag="VGR03_09255"
     CDS             complement(12175..12741)
                     /locus_tag="VGR03_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEV1994507.1"
                     /translation="MKLRIVVSLLVLSTVFGLSTKAQELPKIDASVGYSYFRANPATS
                     GSSGFNLNGGSASASYNFRDWLSAVADFGGYSVGKANGVNVDNHVLTYLFGPQITYRR
                     YRRISPFGHVLVGGARAGSGVFATTNSHTALATAFGVGVDWNVRDRFSIRPLQFDYLL
                     THLPEVTNGSTQTQNNLRVSAGIVFHFK"
     gene            12898..13986
                     /locus_tag="VGR03_09260"
     CDS             12898..13986
                     /locus_tag="VGR03_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994508.1"
                     /translation="MATVAQRRNNSGLHTVYNAAIMSLGDRLGIAGLVVAFFGIGITV
                     LWPDQKWIGWLCLGIAGLLILLWFLMELRILLGFIKSHPKPIAVLALIIGASSCGFLA
                     WRKWMNNPGAPQIAKQNEPKSEEPKREPPKVENPPTVKKKSPVKPVEKSEPFSVSVEW
                     AMFSYGGKGYGTGFWAYYPSQNGCSISPIQAVFFVRVKNLRSTPVTVIGYSLDVFGVP
                     LIRTHMGNIVRIPQKGSIVGPKKPSGYPIEKTINFGQGPGFSIVHFALDESDFTQGFL
                     LQMDLVENLLKAPLQPNIPIRGWAFYQSPNENAFSVAGPGHITLETDDSHTFSYDFDL
                     RNPHPELDILDRVIFLKSFIDLSDCKHP"
     gene            complement(14135..17089)
                     /locus_tag="VGR03_09265"
     CDS             complement(14135..17089)
                     /locus_tag="VGR03_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV1994509.1"
                     /translation="MSPVATPVTQLDRSAVRERVLEVIRGLLDELGSQGALPMLNATS
                     QLDRDLGLGSLERVELLARLETVFDIRLPDRVASDANTPEDLARAILTAPGTNAEAEE
                     TGSALRASVTVQKLHREATDAGVFSAETLIDVLRYRAAHDAERAHLLITEDGDSGERS
                     FTLTFGELYAAGNRCAAELARRGVPVGGRVALMLPTSRAFFVSYTGILLAGAIPVPIY
                     PPFRADRIEEYAARQSAILNNAEVCLLLTFRRAEAVAKLLRPRVRSLSGVADAEKLIE
                     AADKAPPPSPGALPLHVTGSRARQGSDIALLQYTSGSTGDPKGVVLTHANLLANIRAI
                     GEAVQLGPVDVGVSWLPLYHDMGLIGAWLTPLHYGMPVAVMSPLAFLTRPERWLQAFH
                     KHRGTVAAAPNFAYELCVRKIADKDIKGVDLSSWRAALNGAEPVNPETLERFANRFAS
                     YGFRREAQLPVYGLAEAALGVTIPPLNRGPLIDRVERETFTAQGRAVPAAREDETAIA
                     FVSSGNALPQHEVRIVDETGNDVPDRTEGFLWFRGPSATAGYYRNPKATEALLPLGPA
                     TGEEEYAWVNSGDRAYRADGEIYVTGRVKDIIIKGGRNLYPHEVEELAARADGIRKGC
                     IVAFGLTDEATGTEKLVVVAETRERDAARRAALASAVTDLVSRGLGLPPDRVELIPPG
                     SIPKTSSGKLRREETKQLYLAGTLSASRAPAWLQIARLGTGSALRNLGRALLAGVKRG
                     IEILYGVYFVVVFLLWIVPTWVMVQFIKDQKKAGRFTSSALKVLFALVGCQVRVVGKE
                     HMETPGAKIYASNHTSYFDVLPLMLGLGVAYRFVSKMEVGRMPFIGTFLKQMGHLKFD
                     RTDPESRLRQAQEMEEFLRNGESVFMFPEGTFTGEDGVRPFQLGAFKAAVTTGAPIIP
                     VSLAGTRRFLRDGTHLPRPTSVTITLSPPIYPVAAADSSTPADSSGWHELIRLRDAAR
                     AAIVRHAGEPLL"
     gene            complement(17167..17730)
                     /locus_tag="VGR03_09270"
     CDS             complement(17167..17730)
                     /locus_tag="VGR03_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEV1994510.1"
                     /translation="MGLRKPKSIKHNGKSLAEILEAHERFFSGKDGGARADLTGADLS
                     HADLSGANLSGTILRNANLEGTDLRKAKLPGADLSGANLRRADLRNTDMTEAILPGAD
                     LTEAQASGVEFFRCDLSNVNFQRAQLRNVNLRLANVNGAKFSGADMGVAILRETDLTG
                     ADLSGVDLSTTLMPKGFSSAQTAKKSA"
     gene            complement(17802..18164)
                     /locus_tag="VGR03_09275"
     CDS             complement(17802..18164)
                     /locus_tag="VGR03_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994511.1"
                     /translation="MATKIRGIEGMKQGELDFEIQRGAKFVLYQYCISVVILTFRRPS
                     DIYFLRQGENAAVKGLPFTLLSLVAGWWGIPWGPIYTIQSAYNNSRGGKDVTQAVVNS
                     LRAQTAPAPAASPIPPQS"
     gene            18362..19381
                     /locus_tag="VGR03_09280"
     CDS             18362..19381
                     /locus_tag="VGR03_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994512.1"
                     /translation="MPGLSQRVSPCLRASYSRRITIFFLSGVLCAFPFEVLAQEQHED
                     EIVANLAGGRAIVHVAKDVIVFAAIDQPVERNSIPPRVMDLDATHIGILFGASEWRIP
                     ADPKSIRLDRNFQRVAQADPRYQSAPGEGETDLETMGIAFLEKLRPLVAQLHHKIDFS
                     SDDPIFQIVIIGYAPNDYGPEVWVVEFRMEQEQVATRGDYWQTRVQRPRFTQLYPPEK
                     HAPRTIVESRYLPDAKGPTLAELIQGNDPHITHLGSGEPRFAKVLENIYRGQAQKAAP
                     IDSADFMRAALPLIAGGAPFVLGTMDERHGLDWMVPPEEPVEKVQEDKNRPPDAPTLR
                     RTPKP"
     gene            complement(19385..19804)
                     /locus_tag="VGR03_09285"
     CDS             complement(19385..19804)
                     /locus_tag="VGR03_09285"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEV1994513.1"
                     /translation="MARVQNLFRAPKKHLPMEELSEVRAVGDAGIEGCAHARPGRKRQ
                     VLLVDRETLEAMDLRPGIIRENITTDGLNVNSLQIGQLLRVGEARLEVSAICTPCDQM
                     EAIRPGLRKELWGRRGMLCRVLEGGVIRCGDSIEKLF"
     gene            complement(19810..21099)
                     /gene="purD"
                     /locus_tag="VGR03_09290"
     CDS             complement(19810..21099)
                     /gene="purD"
                     /locus_tag="VGR03_09290"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV1994514.1"
                     /translation="MKILVLGGGGREHALVWKLKQSPRVEKIWCAPGNGGIAAEAECV
                     AIDAGDVAAIVALAEKIQPDLTVVGPELPLVNGLTDAFRHRNWAIVGPSQQAAQLEGS
                     KIFSKEFLERHGIPTAKMYGTFDSAADVYTALSSVAWPVVIKADGLCAGKGVFLAPDF
                     AAAKDFVERVMEKNELGPGGRRILLEETLEGEELSFIILTDGKRYAPLVPTRDHKRVF
                     DGNQGPNTGGMGAYSTDELLPDDLRETIRSTIVEPTLHGLAADGMHYQGFLYIGLMLT
                     NSGPRVLEFNCRLGDPEAQAILARLDFDLAEILSDVAAGRLHPSKLHWKPGAGACVVL
                     TSGGYPGKFENGKRIDGLTAVENNTSVKVLHAGTKLAEGTVVTNGGRVLGVTTAAATL
                     EAALASAYEAASKIHFEGMHYRKDIGAHAPRLRAAGD"
     gene            21364..>22707
                     /locus_tag="VGR03_09295"
     CDS             21364..>22707
                     /locus_tag="VGR03_09295"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV1994515.1"
                     /translation="MLIPLRHENMEGRRWPVVTFGLIALNVLIFLGTHWKIEAQEPER
                     IEVRTHVLLLSAMHPELKMPEDVEKFVDDVKSKAPEVAWKQLAATNRKTADSWDAQIR
                     EVEDPEQLQSEMDTLAQRFAEVQKNSILENYAFVPAHPKPISYLTSMFLHAGWLHLIG
                     NMWFLWLAGFILEDRWGRAIYPIFYLITGVGASLFHVMFYPESLAPTLGASGAVAALM
                     GGFLVRFPKLKIEMLWYMLIFRIRFKAAAYWLLPIWLLTEIFYGSIFGQASGVAHWAH
                     VGGFIFGALAALIIARTGLEHNANVVIEDKIGWTADPAVVQGTECMEKGKFDEGIAVL
                     QKHVAAKPDAIDAHSVLRQLYWRKNDIPAHLEATIKLCQLHLKAQDAETAWQDYQEYT
                     NAGGDRMPAATWLELCRIAEGRQDYQRAVSEYEKLAQTYPTERQSLLALLSAGRLA"
BASE COUNT         4995 a         6432 c         6679 g         4601 t
ORIGIN      
        1 aggaaagcat aaatagcaca aaacaaatgc gttacaggct tcgcgatgtg accttcctac
       61 cttgagaatg cccctcgatt cagctatact tttagtgttg tcgatttgct gactagcatc
      121 ctccagatcg acacgtccgc caggtaaaag tgacccagaa attgggctcc gcgccaggag
      181 ctcgcgggcc ttcacctgga gaaacatgag tcttcgccac gtctcgctgc gcgagatggg
      241 atggagcagg aaccctacga gtttcggatt cgtttcgcga ttccgggatc agagggtagg
      301 cgacgtacgg atcattccgc aagcacgcct tccttgcttc acagaaaacg agttttgagc
      361 gcggacccgc actccgaatt gtccgggtct gtccaacgcc gcaggtacat gtgagaagtt
      421 gctgggccag gagaaaccat gggcgacaaa gaaaacgtgg gcggcgcgcc gcaaggcggc
      481 cacaagtacg acgcgaaatc gatcaaggtt ctcggcgggc tcgaagccgt gcgcctgcgc
      541 cccgcgatgt acatcggctc gaccggagag atgggcctgc accatctcgt ctgggaagtt
      601 gtggacaatt cggttgatga agccatggcc ggttacgccg acgagatcaa cgtcaccgtg
      661 catgccgacg agtccgtcac cgtggtcgac aatggacgcg gcattcccgt ggacatgcac
      721 gcgacggaga aacgtcctgc ggcggaagtt gtgttgaccg tacttcacgc cggcggaaaa
      781 ttcgacaacg acacctacaa ggtttccggc ggactccacg gcgtcggcgt ttccgtagtc
      841 aacgcgcttt ccgatcggct ggaactggag atctcgcgcg atggcgcggt gtgggaacag
      901 acttacgaaa agggcaagcc caccaacaag ctgaagcaaa cgggaaaaac gcgcaagacc
      961 gggacgaaag ttacctttca tcctgacccg agcatcttcg ataaaacggt ctacagcttt
     1021 gacacgcttg cgcagcgctt gcgcgaactg gccttcctga ataagggctt gaagattacg
     1081 ctcaccgatg agcgcacgga aaagaccacg gaattcaagt tcagtggcgg catcacggaa
     1141 ttcgtcaagc atctcaatcg cggcaagacg acgctgcacg actcgccgat ttacatcgag
     1201 gggaagcgcg gcaacgtcga gatcgagatc gcgctgcaat acaacgacac ttacggcgag
     1261 aacgtcttcg catttgcgaa caccatcaac accgtcgatg gcggaacgca tctctccggg
     1321 ttccggtcgt cgctgacgcg cacgatcaac acttttgcgt ccggggcagg aatgctcaag
     1381 gagcagaagg acgaagtcac cattaccggt gacgatgtgc gcgaaggcct cgtagcggtc
     1441 gtcagcgtgc gcctgcctca gccacagttt gaaggacaga ccaagggcaa gctgaatagc
     1501 gacatcaagg gcgacgtcga gcaattggtg aacgagagac ttggcgactg gttcgataag
     1561 cattcgacgg ttgcgcggcg agtcatcggc aagtgcatcg aggcggcgcg cgcgcgcgaa
     1621 gcggcccgca aggcgcgcga attgacgcgg cgcaagtccg cgatggattc cagcggcctg
     1681 ccgggaaaac ttgccgattg ccaggaacgc gatccggcgc gctgcgaact gttcgtcgtc
     1741 gagggtgaat cggcgggcgg ctcggcgaag atgggccgcg accggaagta tcaggcgatt
     1801 ctgccgctca agggcaaaat ccttaatgtg gaaaaggcgc gttacgacaa gatgctcggc
     1861 catgaagaaa ttcgcgccat catcacggcg ctgggcaccg gcatcggtaa agacgatttc
     1921 gatgctggaa aactgcgcta ccacaaaatt attctgatga ccgacgcgga cgtcgacggc
     1981 agccacatcc gcacgctgct actgactttc ttcttccgcc acatgaagga attgatcgaa
     2041 cgcgggcaca tctacatcgc gcagccgccg ctctaccgcg tgaagcgcgg caagacggag
     2101 aaatacatcc gcgacgaacg tgattttgcc cgcgagctga tgaagcgcgc cacggaagac
     2161 cacgtcgtga aagccaagga aggcgtgaat ctggaaggcg cgcggcttac ctcattcttg
     2221 ctgaacgtgc aggagtacga agccgccgcc gcaaagcttg gccgcaggct gcgcgaacac
     2281 ggcctcgtcg aattgctcgc cgaaagcggc ctcgagaaga aaacggactt cgaggacaaa
     2341 aaagcgctgg aagaactatt gaagcaactg gaaaagacca agctaaaact tgaaggcaaa
     2401 atcgtcttcg atgaggagca cagcttgtac gaaatccagt ttggcccgcc gcacagccag
     2461 aagatcaatt gggccgtggc gtcgactgcg gaatacaagc ggctgcgcgg gctcgccaaa
     2521 cagatcgaag agttcaacca tccgccgttc accgtggcgc gcaatggcga caaggtgacg
     2581 aaggaaaagg cgaccgacgt tctcaactac gtcgtcgaag acgccaagaa agatttcacc
     2641 atcacacgat tcaagggctt gggcgaaatg aacgccgagc agttgtggga caccaccatg
     2701 aacgcggaca cgcgcacgtt gcttcaggtg aagctcgagg atgccgtcgc tgcagaagat
     2761 attttcacga cgctcatggg cgagaacgtc gaggcgcgcc gcaagttcat cgaagaaaac
     2821 gcgctggaag tcgtgaactt ggatatttag ccagattccg atgctttatc aactgggatg
     2881 ggcgacattg cctgggctgc ggggattgag ttgcagcggg tttcgcgcga cgccgacttc
     2941 ggcgcctgac aacgagcgcg gcgtcgcgat tgaatttgct ggcaacgacg agcgcgatgc
     3001 attcttgcgg cagatcgaag agcattttgc ggagcggcgg tttacgaatt ctgcggatgc
     3061 gttcgacacg gtgaaggcat acgtgctgga acacgcggcc aaacgataga aattgcttgt
     3121 aggggcgccc gcagcggcgt tcctaaaccg gggaatctta gcaacagcga ggggaaatgg
     3181 cggacgagat aaatacatca gtactgctcc cgatcgatat cgaagtagaa atgaggaagt
     3241 cgtacctcga ttacgcgatg agcgtgatca tcgcgcgcgc gctgcccgac gtgcgcgatg
     3301 gtttgaagcc ggtgcagcgg cgcatcctgg tggccatgaa cgacctcggc ctggcctcca
     3361 accgcggcta ccgcaagtgc gccaagattt gcggcgacac ctccggcaac tatcacccgc
     3421 acggcgaagc ggtcatttat cccgcgatgg tgcgtctcgc gcaagacttc aacctgcgct
     3481 acatgctggt cgacgggcag ggaaacttcg gttccgttga cggcgatcca ccagcggcca
     3541 tgcggtacac ggaagcgcgg ctggcgaaaa tctcggaaga cattctcgcc gacctggaaa
     3601 aagaaaccgt cgatttcatt ccgaacttcg atcagacgcg cgaagagccc gtggtccttc
     3661 cgtcgcgcat cccgaatttg ctcgtcaatg gggcgagcgg catcgctgtt ggcatggcca
     3721 caaacatccc gccgcacaat ttgcgcgaga ttgtggacgc agccattctg ctgatcgaaa
     3781 agcccgacac gccgctcaaa gaaataatga agattgtgcc ggggccggat tttccaaccg
     3841 gggcgtacat cgcgggccgc gaaggcatcg aggctgccta ccgcatgggc cgcggcagct
     3901 ttaccatgcg cgccaaagcc gccatcgaag agaccaccaa agaccgcgaa agcatcatca
     3961 tcacggaaat tccgtatcaa gtgaacaaag cgcggctgat cgagcgcatc gccgaattgg
     4021 tgcagaccaa gaagctcgaa ggcatttccg acgtgcgcga cgaatcctcg cgcgaaggca
     4081 tgcgcgtcgt catcgagctg aagcgcggcg aagaatcgca gttgatcctg aacaatcttt
     4141 tcaagttcac gcaaatgcag gaatcgttcg gcatgatcct gctctccatc gtggccgggc
     4201 agccgcgcga gctgggcttg atcgcgatga tcaagctatt tatcgagcac cgcatcgatg
     4261 tgattcgccg ccgcacgatt ttcgagctga agaaagccga acagcgcgag cacattttgc
     4321 tcggctatca gattgctctc gatcacctcg acaacgtgat ccgcatcatc cgcggatctt
     4381 ccagccgcgt ggaagccagg gaaaacctgt tcaagtattt cagcgaacag gacgtcacca
     4441 tcacggaaaa cgggaagccg aagaaaatcg agggcgtgaa gctggacggc cgaaagtacc
     4501 gcgcacaggg agcgccggaa attgaagcgg ctggccatgg aaacgcgctc ggcctcagtt
     4561 acgcgcagat cgacgccatc ctcgaattgc aactgcaccg gctgacgcag ctctccatcg
     4621 acgaaatcgt aaaggaattg aagtccattc gtgaattgat cgccgagttg cgcgagattc
     4681 tggcgagcga caagaagctc aagcaggtca tcgtcgccga gctgcgggaa gtgcacaaag
     4741 cgtacggaga cgatcgccgc acgcagatcg tcgacaaggt cgatgagatc aagctcgaag
     4801 acttgatcgc cgacacggag atgttgatta ccgtcagcca tgcgggctac atcaagcgta
     4861 cggcggcgga tacgtaccgg catcagtcgc gcggcggcaa ggggcgcatc ggggcgagga
     4921 cgaaggaaga agatttcgtc gagcacctgt tcatcgcttc ggcgcacagc tacattttgc
     4981 ttttcacgtc gaagggccgc gtttattggc tgaaggtgta tgaactgcca gaagctgcag
     5041 cagccacgcg cggcaaggcg atttccagcc tggtgaaatt ccaggaagac gaaaaactga
     5101 cggcgctcgt tgcggcgcgc aacctggaag aagcgggcca gttcgtcttc atggccacga
     5161 agagcggcac ggtgaagaag tcggcgctga cggaattctc caatccgcgc tcgaccggca
     5221 tcatagccct cacgttggat ggcaaggacg agctcatcgg cgcgaaactc accgacggca
     5281 agaagatgat tttcctggcc agccacgaag gccaagcgat cctcttccgc gagacggaag
     5341 tgcggccgat gggccgcaat gccggcggcg tgaacgggat ggatctggac aaggaagact
     5401 acgtcgtgag catggacgcg gtgcagccgg acttcgagat catcgccaag gagcatgcga
     5461 ccacgacgca gaatctcgag gaactcgaga acgaacagat caaggactca ctggtgacgc
     5521 tgatgctgac ggtgtccgag aaaggcttcg gcaagcgcac gccgctcgcg gaatatcgca
     5581 tcacgtcgcg cggcggcaag ggcgtcgtga acatgaagac cacggaccgc aacggctatg
     5641 ttgtggccac gctgcaggtg accgaagaat cggacgtgat gatcattgcg cataacggca
     5701 aagtcattcg cgtgcactca aacgaaatcc gcgaagctgg ccgctcgacg cagggcgtgc
     5761 gtttgttgcg gctcgaggac gacgactccg tcgccgccgc cgctgtgctg caggaagaaa
     5821 acgaagaaga gaaaaagtaa atccgcgatc aggaaaacag ctctggggca cgaatcatcg
     5881 tgcccctttt ctgtttcggt ctgcgttaca ctactttccc atgaaaatta cacgccggat
     5941 ctttactttt tcccttctca ttgctccgct tgttttttct gttgcgggtg aatcagccga
     6001 taagcccgct gcaaatcgca agtatctcgt ttttgtcggg acgtacaccc acaagacgga
     6061 gagtaaggga atttacgggt acgagtttga tgcggacacg gggaagctaa caccgaaggg
     6121 cgttgcggtg gagacgctgg acccgtcgtg gatcgcggtg caccccagcg gaaaatttgt
     6181 gtatgccgcg aacgaagcag gcaaggcgag caccgtgagc gcttttgtgg tggatgggaa
     6241 gagcgggaag cttacgttgc tgaatcagaa gccggcgctc ggcgaggatc cgtgttattt
     6301 gtcgttcgac aaaaccggca agtacatcct ggttgccaac tacagttcgg ggacgattgc
     6361 ggttttcccg attttggcgg acggacggct gggagaacac accgcgctgg tgaaagacca
     6421 gggcgcgacg gggccgaaca aaaagcggca ggaagcgccg cacgcgcact ggattgagac
     6481 gacggcgcac aatcatttcg tttacgttgc cgaccttgga ctggatcgcg ttctgatcta
     6541 caagttcgat gcgacgaagg gctcgctgac gccaggagag ccgctgccct cgaaggcgac
     6601 tccggataaa gaggcgccgc tcgatccttt ttctgtgacg ctgaatccgg gcgcagggcc
     6661 gcgacatgtg gcgttcgccc gggacggcaa gttcatgtac gtgctgggcg aattgcagtc
     6721 cacggtgacc gtttttgcga atgaggcgca agagacttat cgtagcgtgc agcagatttc
     6781 cacgctgccg aagggattcg caggacggaa cgacgcggcg gagattgccg tacatcccaa
     6841 tggaaaattt ctttatgcct cgaaccgggg gcacgatagc attgcgattt ttgcgatcga
     6901 tcccgcgaag gggacgctga cgttcatctc gcatgttttg accgggggca aagaaccgcg
     6961 gcactttgct atcgatccga gcggcaaata cctgctggcg gagaaccaat tctcgaacaa
     7021 cgttgttgtg ttcaagattg atcccgcaac gggtggcctg acccctaccg gacaggttgt
     7081 cgaagtcccg tcgcctgttg acatcgcctt tttgcaggag gattgagcct ggctccccgc
     7141 acggctggag cgaacgagtt ccaagttagg aagaacactc cccccatttt ttgtaagtgt
     7201 tgcatctaaa gagcttaagt attgcgcaag tcctttagat gcaacactta cgagatggtc
     7261 tagaagtgtt gcatttaaag tacttaggag ttaacataat cctggagttg gcgtgctcta
     7321 tttgcaaaaa aagcaagatt ccagatgaat tgaatctttc ccgggtttga agaccagaga
     7381 aggccccatc aggaagcagc gcaccccaaa taagaagcgg cagccgaggc tgccgcttct
     7441 tgaaccaggt catagcttaa cctggaagta ttataccaca tccgctctga ccggccgggg
     7501 agagccttct ctttgctctg ggtagttggc ggggtggaag tgcttgcacg ttgagcggtt
     7561 taggttacaa tcccggccaa catggcgaag cgtaaagcta gaaaagtggg agcgcggaag
     7621 ccgaaacgca gcacacggag agcccacggg gcggccgggg gaaaactgcc gaaagaggcc
     7681 gtgacgctaa tcgtcatcct gcgggctcgc gaggggcaag aaacgttgct ggaagcggaa
     7741 ctgcgcgcac tggttggtcc ctctcgaaaa gaagacggct gcctcaccta tagtattcac
     7801 cggtccatcg atacacccgg cgcgcttctg ctacacgaag tttgggcgag ccgcgaggcg
     7861 cacaccgagc atacccatac gccgcatttc ctgcggtgga atgcccgcaa ggacgcactg
     7921 ctcgcgtctc gtgacgccaa cttctggaag cagatcgtct aggatagtcc taggcttttg
     7981 ctgagtagca gctatgagct acgacacgaa aattcttatc ctggtttttg cagggtcctt
     8041 ccctctcgcc gtgctgtatc tgtatttgaa ggactttctc tacaccaaga cgactggtcg
     8101 tcctgcttct caacgctacc gcgagcgcgt tgtcaggtgg tacggcccgt tgacgcgccg
     8161 caaatacatc gcattttgta tctttcttgg actgactacg ctgctttctg cttactcggt
     8221 aggctggagt ggagagtaca gaggctggag caagttaagc ccgctaatcc ggatgatttt
     8281 cgcagctttt cttctctttt ttttacagaa gcgctggcga aagcagcaag caggattgaa
     8341 cgatcagcct tccggtgcgg cgaggaagta gccggggcgg gcgcggacct tcaggttttt
     8401 tgcgtcgttg cgaagcacat tcactttaat ttcgtggtag ccggcggcat ccgagccggt
     8461 cggggtatag ctgatggtgt attgcgagtg cagctcgccg ccgatttcgt cgatggcttt
     8521 ttcgatggag cgatccctaa acgtggcgag gtgggtgccg ccggtggcgg tggcggcgac
     8581 ttcgagggcg tgatccttca cttgatcgtg aacgtgctgg acggcccaga cggccagcgc
     8641 catcaaatcg atattgccgt agcggttttc ttcgctggtg ggcgtttgcg gggtgccggg
     8701 ctgcggcgga agcggaaacg tgccgggtgg ggtgattttc ggccgcgtgt ctttgggctt
     8761 ggcttgcagc tcggcgcgag tcgtggaaag tccaacgctg taaatcgtca cgttggcgag
     8821 ctgggcctgg cgcaggactt cgccgagctt ggcttcgctg ccggcatcgg cggcttcgga
     8881 gagaatcatc agcacacggc ggcgcccagg tttatcggcg gtcgcctgcg gacggccgga
     8941 gagcatctcc acgccgacgg ccatggcgtc gtagagcttc gagcccgaag tccctgttcc
     9001 cagatgcgca atggtgttct cgatcaggtc tccgttagca gtgaaatcct gcagtttgtc
     9061 gaccccgtcg ttaaaactca ccacggcagc ttcgccggtt gaccccatta cagtttgcgt
     9121 gaagagaatg ccggtcttgc gtacttgcgg caggaggggt tcgatgcgcg agctggtctg
     9181 gagcaggatt accagggaga gcgggtcgcc gccgagatcg aagtgggaga tctgctgcgc
     9241 tacgccattg tcagtaatct gaaaatcgcg ggcctccaga ttgtgaacca tgtcgccgcg
     9301 gccggtgcgc acggtcacgg gcgtatttac cagggtgacc tgcactttga ccctggcttg
     9361 ggtgttcgta gggggcaact ggacttcgac gccgggtttg ggcggaagcg gcccgacggg
     9421 agcttgagcg ttggcggggg cgtcagcgag agccagaaga agcgtcagag ttagggatgc
     9481 acctgcgatg cgaatcattc ggttgccttg tagcacagga tttagcctgg gcccgatgca
     9541 gtgcagcatc tgcaggggct gaagcccctg cactacactg tacttgattc gacgcatgtc
     9601 cagcggaaga cgttgccgcg agcggcggcg gttcctacaa tcaaggttcg tctcaacggg
     9661 agaatagacc catgtccgat ggaaagaaac tgaagcccca gcacgcgccg aaagcgccgc
     9721 atgaagatcc agaatttttg aacagtacac cggcgcggcc gatccgcatc ctgggcgaat
     9781 acatccaccc actggtgcag ctcaagagag aggggatcgt ggacactatc gtgatgttcg
     9841 gttcggcgcg catcgaatca cacgaaaccg cgcaggcgcg atgcacgcgg ctgaaaaacg
     9901 agaagacctc gaagatgtcc gcggcaaatc gggccaagca ccgcgcggat gtgcgtcacg
     9961 cgaagagctt gctggagatg tcgcgctatt acgaagaagc gcgccagctt tcgcacaagc
    10021 tgacaacctg gtcgctcacg ctgggaccca ggccgcggcg ctttgtggtt tgttccggcg
    10081 gcgggccggg catcatggaa gcggcaaacc gtggagctta tgaggccgga ggtaaatccg
    10141 tggggctttc gatcgagctg ccgcacgagc aatttgcaaa tccgtacatc agcccggagc
    10201 tgagcttcaa tttcaaatac tttttcatgc gtaaactgtg gttcgcgcag attgcgaagg
    10261 cgctgatcgt gtttccgggc ggcttcggga cgatggacga gctttgggaa atgctgacgc
    10321 tgctgcaaac cggaaaactg gccaagcaca acatcattct gatttatggc cgtaagtatt
    10381 gggatgaaat cctgaactgg aaggccatgg tgcgctgggg cacgattaac gaggacgaat
    10441 acaagatgct gcagttcgcc gataacgtcg acgaagcctt tgaccacatc cgcgcggggc
    10501 tcgagaaata ccacatggct gtcgacccgt ttttacaggc gtactgaacg ttgtgttttt
    10561 cccggcgcag gggaatgacg ttgcgccccg cttttatttt gcaatgcgaa aaataattag
    10621 tgcgacgtta cgagctgttc atcggagaaa acagcttcga agcggcgagt ttcgtcctga
    10681 tcgtgcaaca tcacgatggg aaccacgcgc aagtattgtt tgcaggcatg gcactgcgtc
    10741 acttcgccct gtaaatcctg cagaaaacca tccgtgaaat gatacagcgc acgctcgcgc
    10801 cgggcatttt tcttgtcgcg cgcgccgcaa tgcggacaga tgaattccac cgtgtcctgc
    10861 tgcggatcgc ggtccagagc ggcccagccg gtgcggcaca cacctactgc ttcgagattg
    10921 tgccggtcca tcggggcatt cctcctgaga tccacgtcca caaagaatga gatgcacgag
    10981 ggtgacgccc gggtacggcg cctgtaaatt attcaacaaa atacacttat tagccatttt
    11041 aaggccagaa agcatccttt cctggctgcc gagagcgtac tttctggagc caatgggaac
    11101 ttgtcccaag gcttggtgtc caatgcagtg aggacgcgcg caacctcaca tccctgcgag
    11161 cgcggcgccg gtctcctgga gatctagagc aacccccagg aaatcggcgc cgcaaaattt
    11221 tctgtagttt cgctctgtga tagcgcagag cggtggaggc tcgcagaaaa aaatgtggag
    11281 gtgtgtgatg tcgcccgtgc ccaaaacagt ttctggtgaa atcgccgaag cgagccttgg
    11341 atcactaagc ggatgcctgg tggacggtga cgcggaacag cgcagtcgcg aacgaagcat
    11401 tcgccgccgc tcgttggcta tttcaatcct cttgcagagc gcggttcttg cgacgcttat
    11461 tctggtcccg ctctttggca aagcggagcg cataagcctc accatcgtga ctccgattcc
    11521 gccttactcg ccctaccgcg acgtgtcaca aaatcccggg gagcaacgtc cacgtccgca
    11581 cggcccgcaa aacgtgtgcc gtttctgccc gccgctccgc atccctctca ccatcgcgat
    11641 gcgtgaccag gcttcgagca cgtcggagac ggacgagact cccttcgagg gtcagggcat
    11701 tcccggtgca atcgacggac tcatcccgct ttcggactcg aggcaaagag ttttgccgcc
    11761 tcccaacacg gaacgccagg ggacacgccc gcagctcgtg cacgtcggga cgatcaaccc
    11821 agcgatgctg atccatcgtg tcgaacccgt gtatccagcg ttagccagac agacgcatcg
    11881 agaaggccgt gtggaattgc gggccattat cagtacggac ggctcgattc agtctttgca
    11941 ggttgtctcc ggtgatgcgc tcttcctcat atccgcgagg gaagcggttc agcagtggcg
    12001 ctaccgacca actattcttg acgggcagcc cgtagaggtc gacacctaca tcacggtgat
    12061 ttacaccacg cagcactgaa aactaccgca gatggtgcaa ggggccgtcc gcctgtggcg
    12121 gacggcccct ctgcctgcac cgggcaaccg atacttgttg tttccgccga agaattattt
    12181 gaagtggaaa acgattccgg cggaaacgcg cagattgttc tgcgtttgcg tgcttccgtt
    12241 ggtgacttcc ggcaaatgcg tcaaaaggta gtcgaactgc aacgggcgga tcgagaaacg
    12301 gtcccgcaca ttccagtcaa cgccaacgcc gaaagccgtt gctagcgcag tgtgagagtt
    12361 cgttgttgcg aacacgcccg aacctgcgcg cgccccacct accaaaacat gtccaaaggg
    12421 cgaaatccgg cgatatctcc ggtaagtgat ctgcgggcca aaaagatacg taagtacgtg
    12481 gttgtccaca ttcacgccat tggccttacc cacggaatag ccgccgaaat cggcgaccgc
    12541 gctcagccag tcccgaaaat tataagacgc cgaagcgctg ccaccgttga gattgaaacc
    12601 gcttgatccc gaagtggcag gattggcgcg aaaataagag tagccgacgg acgcatcaat
    12661 cttcgggagc tcctgcgcct tggttgacag tccaaaaaca gtcgaaagaa caagcaaaga
    12721 cacaacgatc ctgagcttca tataaagcct ccgcaaggat tagattttgc tcgcgggcca
    12781 gggaacggct tctacaaccc ccgagtttgc ggcatgccca aggcaggtag gcactaatcc
    12841 gcactttttc tcgtttggaa ttcagatgct gcgagggcac accaggttgc acggaaaatg
    12901 gctacggtgg ctcagcggcg aaataattcg ggattgcata cggtatataa tgccgcaatt
    12961 atgtcactcg gcgaccgtct cggaattgct ggtttagtcg tggccttctt tggtattggt
    13021 ataaccgttc tgtggccaga tcaaaagtgg attggctggc tatgtctcgg aattgcgggt
    13081 ctgctgatat tgttatggtt tttaatggaa cttcgcattt tgctgggttt catcaaaagc
    13141 catcccaaac caatagccgt gctggcacta atcattggcg cgagcagttg cggttttctt
    13201 gcttggcgaa agtggatgaa caatccaggt gctccacaaa ttgcaaaaca aaacgaaccg
    13261 aaaagcgaag aaccaaaaag agaaccgcca aaggtcgaaa atccgccaac ggttaagaaa
    13321 aagtctccag tcaaaccagt cgaaaaaagt gagccgtttt ctgtatccgt cgaatgggcg
    13381 atgttcagtt atgggggcaa aggatatggg acaggtttct gggcatatta tccatcgcaa
    13441 aacggctgta gtatctctcc gattcaagca gtcttttttg ttcgcgtaaa gaatctgcgg
    13501 agtacacctg ttacggtcat tggatacagc ctagatgtct ttggcgtgcc cttaatcaga
    13561 actcatatgg ggaacatagt tcgtattcca caaaaaggaa gtattgtcgg tccgaagaag
    13621 ccgagcggat atcctattga aaaaacgatc aacttcggtc aaggaccagg attttccata
    13681 gttcacttcg cacttgatga aagtgatttt acgcaaggat tccttctgca aatggacctc
    13741 gtagagaatt tgttaaaagc tccgttgcaa cccaacatac caattcgagg ttgggcattt
    13801 tatcaatctc ctaacgaaaa cgcgtttagt gtcgcaggcc caggacatat aaccttagaa
    13861 accgatgatt cgcatacttt ttcctatgac tttgatttga ggaacccgca tccggagttg
    13921 gatatattag atcgggtgat ttttttgaaa tccttcattg acttgtctga ttgtaagcac
    13981 ccctagttag gctgccgagt gggccacaga ttgagcgatg agacggtttt ggctgccgca
    14041 tccctttggg gtttgcttta ccaaagggtg cgggttttga ctttctcttt ctaacttcta
    14101 acctccagca acgaggcttg caggggtcac gcttctacag cagcggctcg ccggcgtgac
    14161 gcacaatcgc cgcgcgcgcg gcgtcacgca aacgaatcag ttcgtgccag ccggaggaat
    14221 cggcgggagt acttgagtct gccgcagcga ctggatagat cggcggcgaa agcgtgatgg
    14281 tgacactggt tggccgaggc aagtgcgtgc catcgcgcag aaaccggcga gtcccggcga
    14341 gagacacggg gataattggc gcgccggtcg tgacggcggc cttgaatgcg ccaagctgga
    14401 agggccgcac gccatcttcg ccagtaaagg tgccttccgg aaacataaat acggactcgc
    14461 cgttgcgcaa gaattcttcc atttcttgcg cctggcgcaa tcgtgattcc ggatcggtgc
    14521 ggtcaaactt cagatgcccc atctgcttca aaaatgtgcc gatgaagggc atccgaccga
    14581 cttccatttt tgaaacgaac cggtacgcga cacccagacc gagcatcagc ggcaacacgt
    14641 cgaaataact ggtgtgattc gaggcgtaaa tttttgcgcc cggcgtttcc atatgctcct
    14701 tgccgacgac gcgcacctgg cagcctacga gcgcgaacaa gaccttgaga gcggaagaag
    14761 tgaagcgtcc tgctttcttc tgatccttta tgaattgcac catcacccag gtgggaacaa
    14821 tccaaagcaa aaacaccacg acgaagtaca caccgtacag aatctcgatc ccgcgcttta
    14881 cgccggcaag gagcgcgcgg ccgaggttgc gcaaggcgct ccccgttccc aggcgcgcga
    14941 tttgcagcca ggctggcgcc cgggaggcgg aaagagtccc cgcaagatag agctgcttgg
    15001 tttcttcgcg gcgcaatttt ccgctggaag ttttcggaat gctgcccggc ggaattaact
    15061 ccacgcgatc cggcggaagc cccaatcccc gcgacaccag gtcagtcacc gcggaagcga
    15121 gagccgcgcg ccgcgccgcg tcacgttcgc gggtctccgc gacgacgacc aacttttccg
    15181 tgcctgtggc ttcgtccgtc aagccgaatg cgacgatgca tcccttgcga attccatcgg
    15241 cgcgcgccgc aagctcctcg acttcgtgcg gatacaaatt gcggccgccc ttaatgatga
    15301 tgtcctttac gcgtccagta acgtaaattt cgccgtcggc acgatacgcc cggtcgcccg
    15361 agttcaccca agcgtattct tcctcgcccg ttgcgggacc cagcggcagc agcgcttcag
    15421 ttgctttcgg attgcggtag tagccggcgg tggccgaggg gccgcgaaac cacaggaaac
    15481 cttccgtgcg gtctggaaca tcgttcccag tttcatccac gatgcgcact tcatgctgcg
    15541 gcagggcgtt tccggaagaa acaaaggcga ttgccgtttc gtcctcgcgg gccgcgggca
    15601 ccgcgcgccc ttgcgccgta aacgtctcgc gttccacacg atcgatcagc ggaccgcggt
    15661 tcagcggcgg gatggtgact ccaagagcag cctccgccag gccgtagacg ggaagctgcg
    15721 cttcgcggcg aaagccgtag ctagcaaagc ggttcgcaaa acgctcgagc gtttccgggt
    15781 tcacgggctc cgcgccattc agtgccgcgc gccaggagct gagatccacg cccttgatgt
    15841 ctttgtccgc gatcttgcgc acgcagagct cataagcaaa attcggcgcc gcagcgaccg
    15901 tgccgcgatg tttgtgaaac gcctgcagcc agcgttccgg gcgcgtcaga aaagcgaggg
    15961 gtgacatcac cgccacgggc atgccgtagt gcagaggggt gagccacgcg ccgatcaacc
    16021 ccatgtcgtg gtagagcgga agccagctca cgcccacatc aaccggcccg agttgaactg
    16081 cctcgccaat cgcacgaata ttcgccagca ggttcgcgtg cgttagcacg acccccttcg
    16141 gatcacccgt tgaaccagaa gtgtattgca gcagggcgat atcgctgcct tggcgcgcgc
    16201 ggcttccggt gacgtgcagc ggcagagcgc cgggcgatgg gggcggagct ttgtccgccg
    16261 cttcgatcag tttctccgcg tccgcgacgc ccgagagcga acgcacacgt ggccgcagca
    16321 atttggcgac ggcttccgcg cggcggaacg tcagcagcag acaaacttcc gcattgttca
    16381 ggatcgccga ctgccgcgcg gcgtattcct cgatgcggtc cgcgcgaaac ggcggataga
    16441 tggggaccgg aatcgcgccg gcgagaagta tccctgtgta agacacaaag aaggcccgcg
    16501 aagtcggcag catcagcgcc acgcgtccgc ccacgggcac accacgccgc gccagctccg
    16561 cggcgcaccg gttccccgcg gcgtatagct cgccgaaggt cagcgtaaag ctgcgttccc
    16621 cggaatcacc atcctcggtg atcagcaggt gcgcacgctc ggcgtcgtgc gccgcgcgat
    16681 accgcagtac atcgatgaga gtctcggcgg agaaaactcc cgcatccgtg gcttcgcggt
    16741 gcagcttctg aaccgtcacc gaagcccgca acgcggagcc ggtttcctcg gcttccgcgt
    16801 ttgttcccgg agcggtcaga atcgcgcgcg ccagatcctc cggcgtgttg gcgtctgaag
    16861 ccacccggtc cggcaagcgt atgtcgaata ccgtttccag ccgcgccagc aactccacac
    16921 gctccaggct gccgagacct aagtcgcggt cgagttgcga ggtggcgttg agcatcggca
    16981 gcgcgccctg gctgcccagt tcatcgagca ggccacggat cacttccagc acgcgttcgc
    17041 gcacggctga gcggtcgagt tgggtgacgg gcgtggccac aggactcacg aaggggttcg
    17101 ttttcttccg cgagaggcta ccacgccaaa acgcggattg ctatccacga gcgagcgaaa
    17161 tcggctctag gcgctcttct ttgcggtttg cgccgatgaa aagcctttcg gcatcagcgt
    17221 ggtggagagg tcgacgccgg agagatccgc gccggtcaaa tccgtctccc gcagaatagc
    17281 cacgcccatg tcggcgccag aaaactttgc gccgttcaca tttgccaaac gcaaattcac
    17341 attgcgcagt tgcgcgcgtt ggaaattcac attcgaaagg tcgcagcgga agaattccac
    17401 gccgctggct tgcgcttcgg tcaaatccgc tccgggaaga atggcttcgg tcatgtcggt
    17461 gttgcggaga tcggccctgc gcaggtttgc gccagagaga tcggcgccgg gaagcttcgc
    17521 cttgcgcaga tccgtgccct cgaggttggc gttgcgcagg atggtgccgc tcagattcgc
    17581 cccgctcagg tcggcgtggg aaaggtcggc gccggtcaag tcggctcgcg ctccgccgtc
    17641 tttgccactg aaaaaacgct catgcgcttc caggatttcc gcgagcgact ttccgttgtg
    17701 cttgatgctt ttcggttttc tgagtcccat gctctgcccg ccatcgatga tatcaggctt
    17761 atagtttact cgatgtggcg gtcatgcgga actccgcgcg gtcagctctg gggcggaatc
    17821 ggggaagcag ccggcgccgg ggcggtttgc gcccgcagcg aattgactac cgcctgcgtg
    17881 acgtctttgc cgccgcgtga attgttgtaa gccgactgaa tagtgtaaat cggcccccac
    17941 gggatgcccc accaaccggc aaccagcgac aacagggtga atggcagacc cttcaccgcg
    18001 gcattttcgc cctggcggag aaagtaaata tcggaaggcc gccggaacgt gaggatgacc
    18061 acggaaatgc agtactgata aagaacgaac tttgccccgc gctggatctc gaagtccagt
    18121 tcgccttgct tcatgccttc aatgccgcga atcttggtag ccatggctga gaagactcta
    18181 ttgctacgtg aacccaattc cgcagtcaag tcagattctt cgccgagctg gcgacagcgc
    18241 agttctgatt gcgcgtcacg ggtgtgatag tgttgggcca gtggtctgct gttgcccgga
    18301 ttggtcgtgt ggcgtgaggg ttcttgcaaa actttcattt cgggcggact cccgacgtgt
    18361 catgccggga ctcagtcaga gagtctctcc atgcctccgc gcgagttatt cgcggagaat
    18421 cacgatcttt tttctatccg gtgtcctctg cgcgttcccg tttgaggttc ttgcacagga
    18481 acagcacgag gacgaaatcg tggccaatct cgcgggtggc cgggccatcg ttcacgtagc
    18541 aaaggatgtc atcgtcttcg cggccatcga tcagcccgtc gagcgcaatt ccattccacc
    18601 gcgggtgatg gacctcgacg ccacgcacat cggcatcctt tttggagctt cagaatggcg
    18661 cattcccgcc gaccccaagt ccatccggct ggaccgcaat tttcagcgtg tcgcacaggc
    18721 cgacccccgt taccagtccg ctcccggcga gggcgagacg gacttggaga ccatgggtat
    18781 cgccttcctg gagaaacttc gccctctcgt cgctcaactc caccacaaaa tcgatttctc
    18841 ttccgatgat cccatctttc aaatcgtgat cattggttac gctcccaacg actacggccc
    18901 ggaggtgtgg gtcgtggagt ttcgaatgga gcaggagcaa gttgccacgc gtggcgacta
    18961 ttggcagacg cgggtgcagc ggccgcgctt cacccagctg tacccgccgg aaaaacatgc
    19021 cccgcgcacg attgtggaga gccgctatct tccggatgcg aagggtccca cattggccga
    19081 gctgatccag ggcaacgacc cgcacatcac gcatctcgga tccggcgagc cgcgctttgc
    19141 caaagtgctg gaaaacattt atcgaggcca ggcgcagaag gccgctccca ttgattctgc
    19201 ggatttcatg cgcgccgcgc tgcctttgat agccgggggc gcgccgtttg ttcttggcac
    19261 gatggacgag cggcacggcc tcgattggat ggttccgcct gaggaacccg tcgaaaaagt
    19321 tcaggaagac aagaaccgtc cgcccgatgc gcccacgctc cggcgaactc ccaagcctta
    19381 atcgtcagaa aagtttttcg atggaatcgc cgcagcggat cactccgccc tccagaacgc
    19441 gacacagcat gccacgccgt ccccacagct ctttgcgaag tccgggacga atggcctcca
    19501 tctgatcgca aggggtgcat atcgcactga cttccagtcg tgcttcgccg acgcggagca
    19561 gttggccaat ctgcaggcta ttcacgttca agccatcggt tgtgatgttc tcgcggataa
    19621 ttcccggtcg gaggtccatg gcctctaaag tctcgcgatc cacgagcaaa acctggcgct
    19681 tgcgtccggg acgcgcatgg gcgcagcctt caatgccggc gtcgcccaca gcccggactt
    19741 cagaaagttc ctccatcggc aggtgcttct tcggagcgcg gaaaagattt tgcactctcg
    19801 ccatggcccc taatctcctg cggctctcag tcggggtgcg tgagctccga tgtccttcct
    19861 gtagtgcatt ccctcaaagt gaattttcga agcagcttca taagcggagg ccaaggcggc
    19921 ttcgagcgtc gcggctgctg ttgtaacgcc aagcactcgg ccgccgtttg tgaccacggt
    19981 gccttcggcg agcttcgtcc cagcgtggag caccttcaca ctagtattgt tctccacagc
    20041 ggttaggcca tcgattcgct taccattctc aaattttccc ggatagcctc ctgacgtcag
    20101 aaccacgcaa gccccggccc caggtttcca gtgcaacttt gacgggtgga ggcgcccggc
    20161 agcaacatcg ctgaggattt cggccaggtc aaaatcgagc cgcgccaaaa tcgcctgggc
    20221 ttcgggatcc cccaaccggc aattgaactc caggactctt ggtccggagt tcgtcagcat
    20281 caggccgata tataagaacc cttgatagtg catgccgtcg gcggccagtc cgtgcaacgt
    20341 cggttcgaca atcgtcgaac ggatggtctc tcgaagatca tccggcagca attcatcggt
    20401 cgagtacgcc cccatgccac ccgtgttcgg gccctggttt ccatcgaaga cgcgcttgtg
    20461 atcgcgtgtc ggtacaagcg gcgcatagcg ctttccatcg gtgagaatga tgaacgatag
    20521 ctcttcgcct tccagcgttt cttccaggag gattcgcctt ccgcccggcc ctagttcatt
    20581 tttttccatc acgcgttcga caaagtcttt cgcggcagcg aagtccggcg ccagaaaaac
    20641 acctttgccg gcgcacaatc catccgcctt aatcaccacc ggccaggcca cggaactcag
    20701 cgcggtgtaa acatctgcgg cagaatcaaa cgtgccgtac atttttgcgg tgggaatgcc
    20761 atgccgctcc agaaactctt tcgagaaaat cttgcttcct tcgagctgcg ccgcttgttg
    20821 agaaggccct acaatggccc aattccggtg acggaatgca tccgtcagcc cgttaaccag
    20881 cggcagttcc gggcccacga cggtcagatc gggttgaatc ttttcggcaa gcgcgacgat
    20941 cgcggcaacg tcaccggcgt cgatggccac gcactccgct tccgccgcga tgccgccatt
    21001 cccgggggcg caccagattt tttccacgcg cgggctttgt tttagtttcc acaccagcgc
    21061 gtgttcgcgt ccaccgccac ccagcacgag aatcttcatc cgcattccct cgtcaacgac
    21121 tctttcgaac gatagacgcc cctcctaaac gtgtcaacct ggcgatgcgc tgatgccttg
    21181 gaaacgtctc ttgaagtggg tcctcgcgat tagcgcttcg cgacgaagac cttcgatgga
    21241 gccgggcggc gtttattaat tggctatacc ctgtaccaga gggcagttac acaggggcga
    21301 atttgccttc tacacttggc agagctattc ctctctatct tttcgatcgg aaggggttgc
    21361 ctgatgctga taccgttgcg ccatgaaaac atggagggcc gccgctggcc ggtcgtcacc
    21421 tttgggttga tcgcgctgaa cgtactgata ttccttggca cgcactggaa aattgaagcg
    21481 caagaacccg aacgcatcga ggttcggaca cacgtcctgc tgctctccgc gatgcaccct
    21541 gaattgaaga tgccggaaga tgtagagaag ttcgtggacg acgtgaagag caaggccccc
    21601 gaggtagcct ggaagcaact cgccgcaacc aaccgcaaga ctgcggactc ttgggacgcg
    21661 caaatccgag aggtcgaaga tccggagcag cttcagtcgg aaatggatac gctcgcgcag
    21721 cgctttgcag aggtgcagaa gaattcgatc ctggagaact acgcatttgt accggctcat
    21781 ccaaaaccta tttcctatct aacctctatg tttctacatg ccggttggct ccatctcatc
    21841 ggcaacatgt ggtttctgtg gctggccggt ttcatcctcg aagatagatg gggccgcgcc
    21901 atttacccta ttttttatct tattaccgga gtcggtgcgt ctctcttcca cgtcatgttc
    21961 taccccgaaa gcctcgctcc tactcttgga gcctcgggtg ccgtagccgc gctcatgggc
    22021 ggatttctgg tgcgcttccc caaactgaaa attgaaatgc tttggtacat gttgatcttc
    22081 cgaattcgat tcaaggccgc ggcctactgg ctgcttccga tttggctgct cacggagatc
    22141 ttctacggca gcatattcgg ccaggcgtca ggagtggctc actgggctca cgtcggagga
    22201 tttatcttcg gggctctagc tgctcttatc atcgctcgaa ccggtttgga gcacaatgca
    22261 aacgtggtca tcgaagacaa aatcggatgg actgcggatc cagccgtcgt gcaaggaacg
    22321 gagtgcatgg agaagggaaa gttcgacgag ggcattgcgg ttcttcagaa gcatgtggcc
    22381 gctaaaccag acgccattga cgctcattcc gtattgcggc agctctattg gcgcaagaac
    22441 gacattccgg cgcatctcga agccacgatt aagctgtgcc agttgcactt aaaagcacag
    22501 gatgcagaga cagcttggca ggactatcaa gagtatacca acgcaggcgg ggaccgcatg
    22561 cctgcggcga catggctgga attgtgccgt attgcagagg ggcgacagga ctaccaacgt
    22621 gccgtgtcgg agtacgagaa actggcgcag acgtatccga ccgaacggca atcgctcctg
    22681 gcgctacttt ccgcgggacg cctggcg
//
LOCUS       DASYVO010000282        10668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_4856, whole genome shotgun sequence.
ACCESSION   DASYVO010000282 DASYVO010000000
VERSION     DASYVO010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 10668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10668
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4856"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VGR03_09300"
     CDS             complement(<1..264)
                     /locus_tag="VGR03_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994516.1"
                     /translation="MSANFAHAAFDNPKESFGQVAVTLLGPKGFPKTKYGALFKRECR
                     KVSAMHSQWKRVYEISVAIAVALTLAACASTDPQSPHPEGFPGD"
     gene            complement(394..729)
                     /locus_tag="VGR03_09305"
     CDS             complement(394..729)
                     /locus_tag="VGR03_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994517.1"
                     /translation="MNEMNVKQAYSLLRALKNNLPGSITVDQKYVTQFDIILDALEKE
                     SGQSLDAFRVPGSELRHTAKSKNTLTGEVSYSESPECHRSFLTMKIDAVLGIFTVEAE
                     RKAIGFNAR"
     gene            complement(756..1391)
                     /locus_tag="VGR03_09310"
     CDS             complement(756..1391)
                     /locus_tag="VGR03_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994518.1"
                     /translation="MNHHIRSGIAIVLVLAFVGISWLLDHRPADWEYVFLMFVVFSSA
                     MQDAMDDEKLRIGKATLLLEEAARKTEHLSREVQQQAKHLSREVQQQAEYLKCHITGE
                     ANLLKSDFALLKYELALAAALQRDEDAPEAPEFVPPYPEPKFVSPEFVPLESPKLEPF
                     DAQKWREERVRVIATALGGHSWWDMSVVTIPFRRLVAYIVSRPGFIRRISR"
     gene            2385..3842
                     /gene="der"
                     /locus_tag="VGR03_09315"
     CDS             2385..3842
                     /gene="der"
                     /locus_tag="VGR03_09315"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV1994519.1"
                     /translation="MPDLLPSLVIVGRPNVGKSTLFNRLTGTRRSIVTNEPGITRDRI
                     YGKAEWRGRALEIVDTGGLVPDDKALIPQEILRQAHVAIKKAALLVLVVDSQAGLTPL
                     DEELARLLRTTGKPFVVAVNKVDAMSQESNANVFHRIGVPVFPIAAEHGTGVDDLLDS
                     ALAQVSVVASVEEEKQPDIIEVAIIGRPNVGKSTLLNRMVGEERSIVSPIPGTTMDNV
                     DLEITRDGRAYRFVDTAGIRRKGKTTLVAEKLSVVMARRGLERCDVALLVVDGEQGVT
                     QGDAQIASYAEESGRSVVIVINKWDLAVVAARDAASRDAATAKGKAKRSPGERHKPEN
                     FDSGRLMFDYEKMLRGKLKFLSYAPIVFLSAKSGERAEKLYPLINQCWEARRRRIATP
                     ALNNWLKDEVDLQRGTTPKARPVRIYYMTQAKIAPPTFLIFTNQKAPLHFSYSRFLEN
                     QLRAKFDFVGTPVRFIQRMRKRDKRSSEGGTHRDE"
     gene            3914..4309
                     /locus_tag="VGR03_09320"
     CDS             3914..4309
                     /locus_tag="VGR03_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1994520.1"
                     /translation="MPSNGTAAQVAAEKKLYRIGEVSRLAALKPFVLRYWETEFPMLE
                     PVKSSSGHRLYRQDDVDMVFRIKRLLYDEGFTIAGARRHLREHDGAAEIESRSIHHAA
                     EEAAQLLSRKMLLDLRDTLRAFLTLLERR"
     gene            4384..4460
                     /locus_tag="VGR03_09325"
     tRNA            4384..4460
                     /locus_tag="VGR03_09325"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4418..4420,aa:Pro,seq:ggg)
     gene            4561..5322
                     /gene="surE"
                     /locus_tag="VGR03_09330"
     CDS             4561..5322
                     /gene="surE"
                     /locus_tag="VGR03_09330"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEV1994521.1"
                     /translation="MPHILLTNDDGYQAEGLRALAAALEGFATVSIVAPSREQSGTAQ
                     SLTLRQPIVCNQINEREWAIDGTPADCVIVALHKLLPEKPDMVISGINHGANLGENVY
                     YSGTVGAAREGALHHIPSVAMSLCSKKTEVKFENSARVARAAAEMILKEGLPDQVLLN
                     VNVPEPWNGSVKFTRQSQKITRNQLTEGKDPRGRSYFWLFEQRIDKDVEPDTDYAAIF
                     SGAVSITPLHLDPTHSESLNHLSHWAAPIATAFRP"
     gene            5361..5690
                     /locus_tag="VGR03_09335"
     CDS             5361..5690
                     /locus_tag="VGR03_09335"
                     /inference="COORDINATES: protein motif:HMM:NF026967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2 fold immunity protein"
                     /protein_id="HEV1994522.1"
                     /translation="MKNTFLAALALAVTVATGFSQTTEESSVRPRDGFVPNAETAVKI
                     GEAVLIPVYGEKQIDSERPFTANLHGDIWIVTGTLNCGAPRCDGGTAIVKISKASGQI
                     LFMMHYK"
     gene            complement(5716..8952)
                     /locus_tag="VGR03_09340"
     CDS             complement(5716..8952)
                     /locus_tag="VGR03_09340"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994523.1"
                     /translation="MKASRRIAFSFVPVLVFLFMAVIALHAHQADRSAAVSPKDRSAA
                     AKPVDHPAVANTVDPKTYGGMKWRLIGPFRGGRALAVTGVASQPNTYYFGAVAGGVWK
                     TTDGGVSWDPLFDKQTTSSIGSIAVSDSDPNVIYVGTGEACIRGNISFGDGVYRSNDG
                     GKTWNNVGLKDTRHIGKVIVHPTNPDVAFVAALGHAYGTNNERGIFRTRDGGKTWEKV
                     LYFDDRTGGIDIVFDPQNSHVLFAAMWEGYRTPWTLNSGGAKDGLYRSNDDGTTWKRV
                     EGNGMPEGPLGRIGVAVSGADSNVIYALIEAKKGGLYRSDDGGTNWALINDDHRFRQR
                     AWYFTHVWADPKDSNKVYIANTGLFRSNDGGKSVDRLNAPHGDHHGLWIDPHNPNRLI
                     NGNDGGATISIDGGKNWSTQGNQPTAQFYHVTADNDFPYRVYGTQQDNSSVGIATASD
                     RGYVDRADWDSVGGGESGYVAVDPRDSHIVYAGSYFGYISRLDRRTNQVQNIQQWPLD
                     PDGYNASVQKYRYTWTMPIVFSPHDANVLYHSSQFLFRSNDGGHSWQTISPDLTRNDK
                     SKQQDSGGPITKDQASIEFYDLIFTVAESPKQKGLIWAGTDDGLIQLTRDDGKTWTNV
                     TPKGFPEWAMISLIEPSSFEAGTAYACVDAHKLDDFKPYVFKTSDFGKTWSPITTGLP
                     DGSYVHAVREDPKRKGLLYAGTETGAWVSFDDGSHWQALQMNLPTTPVHDLIIHGDDL
                     VVATHGRAFWVLDDISPLRQANASIAGEEAHLFASRAATRTRLGHTRRRRYAIGENPP
                     DGAALYYYLKEAPKDPVKLDLLDAQGKVIRAFTSEEKKKDAGSDEGEKDEAEDHIPAK
                     AGLNQFTWDLRYESPKKIPAAIYDEGDPSGPLVLPGNYQARLTVGAKNFTAPLAVTMD
                     PRVKTSAEDLRKQFDLLLKMRDRQDEMNKAILAIRDLRGQLQALEKRLGSDEPAKPVV
                     AAAADLRKKIGAIEEELIQVNSKASEDELNYPTKLNSRIGYLQNAVDSADAQPTEAEF
                     GVYAELDQQLEAQLVKWRDVLAKDVPALNDTMQKSNVPLIAPSSAGVH"
     gene            9058..10212
                     /gene="solA"
                     /locus_tag="VGR03_09345"
     CDS             9058..10212
                     /gene="solA"
                     /locus_tag="VGR03_09345"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HEV1994524.1"
                     /translation="MSSQRTYDVAVIGAGVFGAWTAWHLAKRGQRVALIDAYGPAHSR
                     ASSGGESRIIRMGYGADELYTLWSQRSLEQWKRLFAALKQPLFHETGVLWLGGEDDSQ
                     LRRTAATLRRCKVRFEEFDRAALEEKYPQVSFEDIKTGIFEPHSGVLMARRAVATLVE
                     DALRLGVQYCSAQILKPAGSGRLEQIVTSQGERIGAEQFVFACGAWLGKIFPEVLGAR
                     IFPTRQEVFFFGIPGGDTRFAAPALPTWLFQEDLFYGMPDIESRGLKIAYDGHGECVD
                     PDTQSRIVTPVMRERVRTYVERRFPALKDAPIVETRVCQYENTSNGDFLIDQHPGMEN
                     VWFVGGGSGHGFKHGPALGEYVTGRLLDRAEPEPRFSLATKARTQNRAVY"
     gene            10427..>10668
                     /locus_tag="VGR03_09350"
     CDS             10427..>10668
                     /locus_tag="VGR03_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994525.1"
                     /translation="MVLKTDRAARAFALLAALMTIVAAGASGQDIKIAYEKFVLPNGL
                     TVIVHEDHKAPIVALNIWYHVGSKNEKPGKTGFAHL"
BASE COUNT         2213 a         3018 c         3143 g         2294 t
ORIGIN      
        1 gtcgccggga aacccctccg ggtgtggcga ctgaggatcg gtcgacgcac acgcggccag
       61 cgttagtgcc actgcgattg ccacgctgat ttcataaacc cgcttccact gagagtgcat
      121 cgcgctcacc tttcggcatt cgcgtttgaa cagcgcaccg tacttggtct taggaaagcc
      181 ctttggaccg agcaacgtca cagcgacctg accgaagctt tctttgggat tgtcaaaggc
      241 ggcgtgtgca aagttcgcgg acatgcaact tgagctagag tgagtttcgc tttcggaggc
      301 ttggtcgctt tcttcacacg actcataaat ggagtctaca ataaggaagg tggccctgct
      361 aggcgctccg gcaggttgac ttggctgaag gaactatcgt gcgttgaaac ctattgcctt
      421 gcgttccgcc tcaaccgtga agatgcccaa gactgcatca atcttcatcg tcaagaagga
      481 gcgatgacac tctggactct cgctatagct gacttcgcct gtaagcgtgt ttttgctttt
      541 agccgtgtgg cgcagttccg accccggtac tcggaaggcg tcaaggctct ggccggattc
      601 cttttctagc gcgtccagaa tgatatcgaa ctgagtgaca tacttctgat cgacagtgat
      661 gctgccgggc aggttgttct tgagtgctcg aagaagtgaa tacgcctgtt ttacattcat
      721 ctcgttcatt ggcgaagtat accatcaacg ctagttcacc tgcttatccg gcggataaaa
      781 ccaggacggc tcacgatgta cgccaccaag cggcgaaaag gaatcgtgac cacgctcata
      841 tcccaccaac tatggcctcc aagcgcagtc gcgatcacgc ggactctttc ttcccgccat
      901 ttctgtgcgt caaacggctc tagcttcggc gactccagcg ggacaaactc aggcgaaaca
      961 aacttcggtt ctgggtacgg cggtacaaac tccggtgctt ctggtgcgtc ctcgtctctt
     1021 tgcagcgcag ccgccagcgc aagctcgtac ttcaacagcg caaagtcgga cttcagtaga
     1081 ttcgcctcac ccgtaatgtg gcacttcaag tattctgcct gctgctgaac ctcccgactc
     1141 aagtgctttg cttgttgctg gacctctcga ctcaaatgtt ctgtcttgcg tgcagcttcc
     1201 tcaagcagca gcgttgcttt ccctattctc agtttctcgt cgtccatcgc atcctgcata
     1261 gctgaactga agacaacgaa catgaggaag acgtactccc aatccgcagg ccgatgatcc
     1321 aggagccagc taatcccgac aaaagccaga acaagaacta tggcgatgcc agaccgtatg
     1381 tggtgattca tgtcggaagg acctcccttt acttgcgatg gcatcatagt gctcaatgaa
     1441 cgtgcgcggc aatgcaaccc ggtgcggcgc ccgagcacat caagattggc ttctacgtat
     1501 tagactccag gcgggtcgca ggcctttggc tcctgagcaa gctgcgggtg ccgcttcggt
     1561 ttttaaagcg tgcgggtgtt gattttcttc gcatcatctt gccgcactct cttttctcaa
     1621 gaattcccct ttcccgctca ggatccccat ccccgaattt caatcttcaa actcgtaacc
     1681 tgcaaatgcg cattttgcat cccggccgct taagccggga catttcaaca tcttaccact
     1741 ccattcgaat gatatctcaa taattacttg acatgcgctt agtattcgtg ctatagttcc
     1801 gcgtgtttgc caagttccac tcccgccgat ctccccattc cttcctcaga cacacccctg
     1861 ttccctcttc cccatcctct aactcccatg gaatcatatc gtttaccgac ccccacccct
     1921 taaaccctgt cgtatcatat cgttacaaaa acataggggg taggggggcc ttcatcactc
     1981 cggcatccgt ccttcacagc ccatcactaa gttctttaga tgcaacactt atagacgcct
     2041 tcgtatgtgt tgcaaacaaa ggacttatga taacgctaag ccctttagat gcaacactta
     2101 caaaaaacag agggtgggga gtgccatggt tgactagcca actttccact cgaccctctc
     2161 gcgggagccg tttcgatgcc tcttccggct ctgtctccgt gaacggcgcc ggccccggcc
     2221 gggtcggggc gttaattccg tctctttttt ccgactttca acttttaact ttcaactttc
     2281 aacctcccca tcttccgaat ccttccgatg caacccggaa tgcgctacat tggaaggttg
     2341 cgcaccacgc cgcggcgccc atagtgcgcc cggcccagaa acttatgccc gatcttctcc
     2401 catcgcttgt catcgtcggc cgccccaatg tgggcaagtc cacgctcttc aaccgtttga
     2461 ccggcacgcg ccgctccatc gtcaccaacg agccgggcat cacccgcgac cgcatctacg
     2521 gcaaagcgga gtggcgcggc cgggcgctgg aaatcgtcga taccggcggc ctcgtcccgg
     2581 acgataaggc gctgatcccg caggaaatcc tgcgccaggc gcatgtggcc atcaaaaagg
     2641 ccgcgctgct cgttctggtg gtcgatagcc aggcgggtct tacacccctc gacgaagaat
     2701 tggcgcggct gctgcgtacc acgggcaaac cgtttgtagt agcggtcaac aaagtcgacg
     2761 ccatgtccca ggaatccaac gccaacgttt tccatcgcat tggcgttccg gtcttcccca
     2821 tcgccgccga gcatggcacc ggcgtggacg atctcctcga ttccgcgctc gcgcaggtca
     2881 gcgtcgtcgc ctccgtcgag gaagaaaaac agcccgacat catcgaagtc gccatcatcg
     2941 gaaggcccaa cgtgggcaaa tccacgctgc tgaaccgcat ggtcggcgaa gaacgctcca
     3001 tcgtttcccc cattcctggg acgaccatgg acaacgttga tttggaaatc acgcgcgatg
     3061 gccgcgcgta tcgcttcgtg gataccgccg gtattcgccg caagggcaaa accaccctgg
     3121 ttgcggaaaa actcagcgtg gtcatggccc gccgcggctt ggagcgctgc gatgttgctc
     3181 tcctcgtcgt cgacggcgaa cagggcgtga cccaaggcga cgcgcaaatc gccagctacg
     3241 ccgaagagtc cggccgctcc gtcgtgatcg tcatcaacaa gtgggatctt gccgtcgttg
     3301 ccgctcgcga tgccgcgtcg cgtgatgccg caaccgcgaa aggcaaagcc aagcgctctc
     3361 ccggcgaacg gcacaaaccg gaaaacttcg attccggccg gttgatgttc gactacgaaa
     3421 aaatgctccg cgggaaattg aaattcctga gctacgcgcc gatcgttttt ctctccgcga
     3481 aatccggcga gcgtgcggaa aaactttatc cgctcatcaa tcagtgctgg gaagcgcgcc
     3541 gccgccgcat cgccacgcca gctctcaaca attggctcaa ggatgaagtc gacctgcagc
     3601 gcggcaccac gccaaaggcc cgccccgtcc gcatctatta catgacgcag gcgaaaatcg
     3661 cgccgccaac gttcctcatc ttcaccaacc agaaggcgcc gctgcacttc agctattcgc
     3721 gcttcctaga gaatcagctt cgcgccaaat tcgatttcgt cggcacaccc gttcgcttca
     3781 tccagcgcat gcgcaagcgc gacaagagat cctccgaggg cggcacccac cgtgacgaat
     3841 gaacgcctcc ctctacagcg atcccccgca aactgctacg atagcggcgt cccccttccg
     3901 acagggcttt cagatgccaa gcaacggcac agcagcgcaa gtcgcagcgg agaaaaaact
     3961 ctaccgcatc ggcgaagtca gccgtctggc ggcgttgaaa cctttcgttc tgcgctactg
     4021 ggaaaccgag ttccccatgc tggagccggt caagagctcc agcggccatc gcctctaccg
     4081 ccaggatgac gtggacatgg tcttccgcat caagcggctg ctctacgacg aaggattcac
     4141 catcgcgggc gcgcggcggc atctccgcga gcacgatggc gccgccgaaa tcgagagtcg
     4201 aagcatccat catgcggcgg aagaggcggc gcagcttctg agccgcaaaa tgctgctgga
     4261 cctgcgcgat acgctccgcg ctttcttgac gctgctcgaa cggcggtgat accttggagg
     4321 tgctgattcc ctctccgatt cggccttctg atctttttgg cctgtcgcgg tcagcgtacc
     4381 ggtcgggacg tagcgcagcc tggtagcgcg cacgcttggg gtgcgtgagg tcgctggttc
     4441 aaatccagtc gtcccgacca tttcttcttc ggacaccgaa acgccaccct tcacccccga
     4501 agaccgtaag ccgcgtactc tgggtgtctt gaaagaaaaa agcatcgaaa atattcgccc
     4561 atgccgcaca ttcttctaac taacgacgac ggatatcagg cggaggggct gcgggcgctg
     4621 gctgcggcgc tggaaggatt tgccacggtg agcatcgttg cgccgagccg ggagcagagc
     4681 gggacggcgc agtcgctgac gctgcgccag ccaatcgtct gcaaccaaat caatgagcgg
     4741 gaatgggcca ttgacgggac gccggcggac tgcgtgatcg tcgcgctgca caaacttttg
     4801 ccggaaaagc cggacatggt gatttccgga atcaaccatg gcgctaactt aggagaaaac
     4861 gtttattatt caggaacagt tggagcggcg cgcgaaggcg cgctgcatca cattccgtcg
     4921 gtggcgatgt cgctgtgctc gaaaaagacg gaagtgaaat ttgaaaactc cgcgcgcgtg
     4981 gcgcgcgccg ccgcggaaat gattttgaag gaaggattgc cggaccaggt tttgctcaac
     5041 gtgaatgtgc cggagccgtg gaacggaagc gtgaagttta cgcggcaatc gcagaaaatc
     5101 acgcgcaacc agcttacgga aggaaaagat ccgcgcgggc gcagttattt ttggctcttc
     5161 gagcagagaa tcgacaagga cgtggagccg gacacggact acgccgcgat tttttccggc
     5221 gccgtctcca tcacgccgct gcacctcgat cccacgcact cagaatcgct caatcacctc
     5281 tcgcactggg ctgcgccgat cgcaaccgcg tttcggccat gagtcgtggt cacaatattc
     5341 attcgcggag ggcgcgaaat atgaaaaata ctttcctcgc ggctttggct ctggcggtga
     5401 cagttgcgac cgggttttcg cagaccactg aagaatcatc tgtccgaccg cgggatggat
     5461 tcgtgccaaa tgcggaaacc gccgtgaaaa ttggtgaggc cgtgttaatc cctgtctatg
     5521 gagaaaaaca gatcgattct gagcgcccgt tcacggcgaa tctgcacgga gacatctgga
     5581 tagtcacggg aactctgaat tgtggcgcgc cccgatgtga cggcggaacc gctatcgtaa
     5641 aaatttcaaa ggcttccgga cagatactat tcatgatgca ctacaaataa tccgcatgtc
     5701 tgcgggagcc agctttcaat gcaccccggc ggatgaaggg gcgataagcg ggacgttgct
     5761 tttttgcatg gtgtcgttaa gggcggggac gtctttcgca aggacgtcgc gccactttac
     5821 gagctgcgcc tcgagctgct gatcgagttc ggcgtagacg ccgaactcgg cttcggtggg
     5881 ctgggcgtcg gcgctgtcga cggcattttg caggtagccg attctactgt tgagcttcgt
     5941 cgggtagttg agttcgtcct cgctggcttt tgaatttacc tggatgagtt cttcctcgat
     6001 cgcgccgatc ttcttgcgga gatcggcggc agcggctaca accggctttg ccggttcgtc
     6061 cgaaccgagg cgcttttcga gcgcttgcaa ctggccgcga aggtcgcgga tcgcaagaat
     6121 ggctttgttc atttcgtcct ggcggtcgcg cattttgagc agcagatcga actgcttgcg
     6181 caggtcctcg gcggaagttt tgacgcgggg atccatcgtc actgcaagag gagcggtgaa
     6241 attcttcgct ccgacggtca agcgcgcttg atagttgccc ggaagaacga gcgggccgct
     6301 cgggtcgcct tcgtcataga tggcggcggg gattttcttg ggagattcgt atcgcaaatc
     6361 ccaagtgaat tgattcaatc cggccttcgc ggggatgtgg tcctcggctt cgtctttttc
     6421 cccctcgtcg gagccggcgt cttttttctt ttcctcgctg gtgaaagcgc ggatgacttt
     6481 gccctgggca tccagtaaat ccagcttgac gggatctttg ggcgcttctt tcaaatagta
     6541 gtagagcgcc gcaccgtcgg gaggattttc tccgatagcg tagcggcggc gtctggtatg
     6601 tcccaaccgc gtgcgagtgg cggcacgaga cgcgaacagg tgcgcttctt cgccggcgat
     6661 ggaggcgttc gcctggcgca gcgggctgat gtcatcgagc acccagaacg cgcggccgtg
     6721 agtggcaacc accagatcgt cgccgtggat gatgaggtcg tggaccggag tcgtcggcag
     6781 attcatctgc agcgcctgcc agtgcgagcc gtcatcgaag gaaacccacg cgcccgtttc
     6841 cgtgccggca tagagcaggc ccttgcgctt gggatcttcg cgaacggcgt ggacgtaaga
     6901 gccgtcgggc aggccagtgg tgattggcga ccaagtctta ccgaagtcgc tggtcttaaa
     6961 aacgtagggc ttgaaatcat cgagcttgtg ggcgtcgacg caggcgtaag cggtgccggc
     7021 ttcgaacgac gacggttcga tcaagctgat catggcccat tcgggaaagc ctttcggagt
     7081 gacgttggtc caagtttttc cgtcgtcacg cgtgagttga atcaagccgt cgtcggtgcc
     7141 ggcccagatc agaccttttt gtttcggcga ttccgcaacg gtgaagatca ggtcgtagaa
     7201 ttcgatgctg gcctggtctt ttgtaatggg gccgccggaa tcctgttgct tcgatttgtc
     7261 gttgcgcgtg aggtccgggc tgatggtttg ccaggaatgg ccgccatcat tcgaacggaa
     7321 gaggaactgc gacgaatggt agagaacgtt ggcgtcgtgt ggagaaaata cgattggcat
     7381 cgtccaggtg tagcggtact tctgcactga agcgttgtag ccatcaggat cgagcggcca
     7441 ctgctggatg ttctgcacct gattggtgcg gcggtcgagg cgcgaaatgt agccgaagta
     7501 ggaaccagcg taaacgatgt gtgaatcgcg cgggtcgacg gcaacgtagc cgctctcgcc
     7561 tccgccgacg gagtcccaat cggcgcggtc aacataaccg cggtcgctgg cggtggcgat
     7621 acctacggat gaattgtcct gctgcgtgcc atacacgcgg tacggaaaat cgttgtccgc
     7681 ggtcacgtgg tagaactgcg ccgtcggctg attcccttgc gtggaccaat tcttgccgcc
     7741 gtcgatggag atggttgcgc cgccgtcatt gccgttgatg aggcgattgg gattgtgggg
     7801 atcaatccac aagccatggt ggtcgccgtg gggagcgttc agacggtcga cggatttgcc
     7861 gccatcgttc gaacggaaaa ggccggtgtt ggcgatgtag accttgttcg aatccttggg
     7921 gtcggcccaa acgtgggtga aataccaggc gcgctggcgg aaacgatgat cgtcgttgat
     7981 cagggcccaa ttcgtgccgc cgtcatcgct gcggtagagg ccgcctttct tcgcttcgat
     8041 cagcgcgtaa atgacattgg aatcggcgcc ggagacggcc acgccgatgc ggccgagcgg
     8101 gccctcgggc atgccgttac cttcgacgcg cttccaggtg gtgccgtcgt cgttcgaacg
     8161 gtagaggcca tccttggcgc cgccactgtt gagcgtccag ggcgtgcggt agccttccca
     8221 catggcggcg aagaggacgt gggaattctg cgggtcgaaa acgatgtcga tgccgccggt
     8281 gcggtcgtcg aaatagagaa ctttctccca ggttttgccg ccatcacgcg tgcggaagat
     8341 gccgcgctca ttgttcgtgc cgtaggcatg gccgagagca gcgacgaagg cgacatcggg
     8401 attcgtagga tggacgatga ccttgccgat gtggcgcgtg tctttcaacc caacgttgtt
     8461 ccaggtcttg ccgccgtcgt tcgaacgata aacgccatcg ccaaaggaga tgttgccgcg
     8521 aatgcaggct tcgccagtgc cgacatagat cacgtttgga tcggagtcgg agaccgcgat
     8581 gctgccgatg gaggaggtgg tctgcttgtc gaagagagga tcccaggaaa cgccgccgtc
     8641 cgtggttttc caaacgccgc cggcgacggc gccaaaatag taggtgttcg gctggctcgc
     8701 cactcccgtt acggccaggg cccggccacc acggaagggg ccgatgaggc gccatttcat
     8761 tccgccgtag gttttgggat caaccgtatt ggcgaccgca gggtggtcga cgggcttggc
     8821 agccgcagaa cggtcttttg gactgacggc cgctgagcgg tcggcttggt gtgcatggag
     8881 agcaatcacg gccataaaga ggaaaacaag aacaggaacg aaggaaaaag caatgcggcg
     8941 agacgctttc acacacacct ccggaatgag gacacaaaac gcaagatggt aacgctacag
     9001 cgcgttggat ggaagagttt ttcctatagg tacagcttgc gataggatgt tccagcggtg
     9061 agtagccaaa gaacatacga cgttgcggtg atcggcgcgg gcgttttcgg cgcgtggacg
     9121 gcgtggcacc tcgcgaaacg gggccagcgc gtggcgctga ttgatgccta cggtccggcg
     9181 cattcgcggg ccagctccgg gggtgaatcg cgaatcattc gaatgggata cggcgcggat
     9241 gagctgtaca cgctttggtc acaacggtcg ttggagcagt ggaaaagact ttttgcggcg
     9301 ttgaaacagc cgctctttca tgaaacgggc gtgctctggc tgggcggcga agatgattct
     9361 caactgcggc gaacggcggc tacattgagg cgttgcaagg tgcgatttga ggaatttgat
     9421 cgcgccgcgc tcgaagaaaa atatccgcaa gtatcgtttg aagacatcaa aacagggatt
     9481 tttgaacccc atagcggtgt tctgatggcg cgccgcgcgg tggccacgct ggtagaagac
     9541 gcgctgcggc tgggcgtgca gtattgcagc gctcagatcc tcaagccagc gggatcggga
     9601 cgactggaac agattgtaac gagccagggt gagcggatcg gcgcggagca atttgttttc
     9661 gcttgcggag catggcttgg caaaatattc ccggaagttc ttggcgcacg gatcttccct
     9721 acgcggcagg aagttttctt ttttggcatt cccggcggcg acacgcgatt tgcagcaccc
     9781 gcgctgccaa cgtggttgtt tcaggaggat ctcttctacg gaatgccgga tattgaaagc
     9841 cgaggattga agattgcgta tgacgggcat ggggagtgcg tcgatccgga cacgcagtcc
     9901 cggatcgtta caccggtgat gagggaacgg gttcgaacgt atgtggagcg gcggttccct
     9961 gccttgaagg atgccccgat cgtggaaacg cgagtctgtc agtacgagaa cacctcgaat
    10021 ggagattttc tgattgacca gcatcccgga atggaaaacg tgtggtttgt gggcggcggc
    10081 tcgggacacg ggttcaagca tggaccggcg ttgggggaat atgtaacggg gagactgctg
    10141 gaccgcgccg aacccgagcc acggttttca ctggcaacca aggcgagaac gcagaatcgc
    10201 gcggtatact gactgtcccg gcccgaaaca cgatggcctg ttgaaacttc cagctgcgcc
    10261 atgcgtcttg tactaaaggg tggttcttcg tttgcctatt ggaatcatca tatcgcaaat
    10321 aacatgcgcg gctcgcctca gaaggcgagc cctacgggct accggcaggc gggatcgatt
    10381 ttattgagaa atacagacta gcgaggttag aaaatacgga ggggaaatgg ttttgaaaac
    10441 tgatcgagcc gctcgcgcat tcgcgctgct cgcggcgctg atgacgatag tggcggcagg
    10501 cgcatcaggg caagacatca agattgccta tgagaaattt gtgcttccga acgggctgac
    10561 cgtgatcgtt cacgaagatc ataaggcgcc gatcgtagct ctaaacatct ggtatcacgt
    10621 gggctccaag aacgagaagc cggggaagac cgggtttgcg cacctatt
//
LOCUS       DASYVO010000283         2996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_51732, whole genome shotgun sequence.
ACCESSION   DASYVO010000283 DASYVO010000000
VERSION     DASYVO010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51732"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VGR03_09355"
     CDS             <1..329
                     /locus_tag="VGR03_09355"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEV1994526.1"
                     /translation="FYIYTSSVGNNGSAFAGLNGNTMWFNTSGAAAMLLGRFFMVIPV
                     LAIAGNLAKKKIAPASAGTFPVTGALFTALLVSTILIVSALTFFPALSLGPILEHLQM
                     LAGKVF"
     gene            337..2385
                     /gene="kdpB"
                     /locus_tag="VGR03_09360"
     CDS             337..2385
                     /gene="kdpB"
                     /locus_tag="VGR03_09360"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEV1994527.1"
                     /translation="MTEHLQHKAISLTDSKILRRALLDSFRKLDPRTMIKNPVMFVVE
                     AGALLTTIQLVRDHLHHAGSFGWGLQITVWLWFTVVFANFAEAMAEGRGKAQADTLRK
                     ARAETQARRLRGDGSIETIASSKLRSGDTVVVVAGEFIPGDGEVIEGVASVDESAITG
                     ESAPVIRESGGDRSAVTGGTRVLSDEIKIRITSNPGETFLDRMIRLVEGASRQKTPNE
                     IALNILLAGLTIIFLLAVMTLQPFAIYSGAPQNIFVLVSLLVCLIPTTIGGLLSSIGI
                     AGMDRMIQRNVIAMSGRAVEASGDVDVLLLDKTGTITLGNRQATSFHCCPNVTETDLA
                     SAAQLASFADETPEGRSIVVLAKEKYGLRGRELAPHNPNFIPFSAQTRMSGVDLDGHE
                     IRKGAPEAIEKYVAERGQVVPAEIKEITREISNSGGTPQLVTQNRRVLGAIALTDVVK
                     GGIRERFDHLRAMGIRTMMITGDNPLTAAAIARQAGVDDFLAEAKPEDKMALIKREQA
                     KGKLVAMTGDGTNDAPALAQADVGVAMNTGTQAAKEAGNMVDLDSNPTKLIEIVEIGK
                     QLLITRGALTTFSVANDVAKYFAIIPAMFAGAFPVLNALNVMHLRTPESAILSAVVFN
                     ALIIIALIPLALRGVRYRPLGAAALLRRNLLIYGVGGVIAPFFGIKLIDMLLTSLGMA
                     "
     gene            2394..2975
                     /gene="kdpC"
                     /locus_tag="VGR03_09365"
     CDS             2394..2975
                     /gene="kdpC"
                     /locus_tag="VGR03_09365"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF001454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HEV1994528.1"
                     /translation="MKKNLLISLWFTLVTTVMFGLIYPLVVTGLSQLLFPGKANGQLI
                     ERNGKLVGSRIIGQAFTGPSYFHSRPSAAGTGNGYDPTSSSGSNLGPTSKVLLDRVNG
                     DVQKLHEENSGSPIPVDLVTTSGSGLDPDVSPAAAEFQISRVARERHLSEPELRSLVA
                     KHMLGRQFGFLGEPRVSILELNLDLDAQHPLSK"
BASE COUNT          621 a          889 c          865 g          621 t
ORIGIN      
        1 tcttctacat ctatacctcg tcggtgggca acaacggctc ggcattcgcg ggcttgaacg
       61 gcaacaccat gtggttcaac accagcggag cagcagccat gttgctgggc cgcttcttca
      121 tggtcattcc ggtgctcgcc atcgctggaa atctggcaaa gaaaaagatc gcccccgcat
      181 ctgcagggac gtttcccgtg accggcgcgc tgttcacagc tttgctcgtt tccaccattc
      241 tcattgtgag tgcgctcaca ttttttccgg cgctcagtct gggcccgatt ctcgagcact
      301 tgcagatgct tgccggaaag gttttctgag gccgccatga ccgaacatct acagcacaaa
      361 gcaatatcgc tcaccgattc aaagattctc cggagagccc tgctggactc attccgcaaa
      421 ttggatccac gaacgatgat caagaacccg gtgatgttcg tcgtggaagc cggcgcgctg
      481 ctgaccacca ttcaacttgt gcgcgaccat ctgcaccacg cgggcagctt cggctggggt
      541 ctccaaatca ccgtgtggct gtggttcacc gttgtgtttg cgaatttcgc tgaggccatg
      601 gcggaaggac gcggcaaggc gcaggccgac acgctgcgca aagcacgagc ggagacgcag
      661 gctcgtcgtt tgcgcggaga tggatcgatc gagacgattg caagctccaa gctgcgctcg
      721 ggagacacgg ttgtcgttgt tgctggagag ttcatcccag gcgacggcga agttatcgaa
      781 ggcgtggcgt cggtcgatga atcggcgatc accggggaat ctgcgcccgt gattcgcgaa
      841 tcgggcggcg accgctcggc ggttaccggc ggcacgcgcg tgctttccga cgaaatcaaa
      901 atacgcatca cttccaatcc gggcgagacg ttcctcgacc gcatgatccg ccttgttgaa
      961 ggcgcctccc ggcaaaaaac gccgaacgaa atagccctaa atatccttct tgcggggcta
     1021 acgatcatct tcttgttggc ggtcatgacg ctccagccgt ttgcgattta ttccggagcg
     1081 cctcagaaca tcttcgtgct ggtctcgctg ttggtctgct tgatccccac aaccatcgga
     1141 ggactgctct cctccatcgg gattgcgggg atggaccgca tgattcagcg aaatgtcatc
     1201 gcgatgtcgg gccgcgccgt tgaagcatct ggcgacgtgg acgtgctctt gctcgacaag
     1261 acgggcacga tcaccctggg gaaccggcag gctacttcgt tccactgctg cccgaacgtg
     1321 acggagacgg atctcgccag cgccgcgcag ctcgcgtcgt ttgcggatga aacgccggaa
     1381 gggcgctcca tcgtcgtcct tgccaaagaa aagtatggtc tgcgcggccg tgaactggcc
     1441 ccacacaacc cgaatttcat accgttttca gcgcagacac gcatgtccgg agtggacctg
     1501 gacggccatg aaatccgcaa gggcgcaccg gaagctatcg agaaatacgt ggctgagcga
     1561 ggccaagtcg tgccagccga gatcaaagag atcacccgtg aaatttcgaa tagtggcggc
     1621 acacctcagc tggtgactca gaatcgccgc gtgctcggcg ccatcgccct gacggacgtc
     1681 gttaaaggcg gcatacgcga gagatttgat catttgcggg ccatgggcat ccgcacgatg
     1741 atgatcacag gtgataaccc gcttaccgct gctgccatcg cacgccaggc cggcgtcgac
     1801 gattttcttg ctgaagccaa gccggaagac aagatggcgc tcattaagcg cgaacaagcc
     1861 aaaggcaagc tcgtggccat gacgggtgac ggcacgaatg acgcaccggc gctggcgcag
     1921 gccgatgtcg gagtcgccat gaacaccggc acgcaagcgg cgaaggaagc aggcaacatg
     1981 gtcgacctcg attcaaatcc aacgaagctc atcgagatcg tggaaatcgg gaagcagttg
     2041 ctcatcaccc gtggcgcact taccacattt tctgtggcga atgacgtggc aaagtatttc
     2101 gccatcatcc cggcgatgtt cgccggcgca tttccggtgt tgaacgcgct gaacgtaatg
     2161 cacctgcgca cgccggaatc cgcgatcttg tcggcagtcg tcttcaacgc cttgatcatc
     2221 attgcgctga tcccgctggc gctgcgtgga gtgcgctatc ggcccttggg tgcagcggcc
     2281 ttgctgcgcc ggaatctgct gatctacggg gtcggcggag tcatcgcgcc ctttttcggc
     2341 atcaagctca ttgacatgct gcttactagc ctgggaatgg cctagaggaa ctcatgaaaa
     2401 aaaatctgct catctccctg tggttcaccc tggtgaccac ggtcatgttc ggtctgatct
     2461 atccgctcgt tgtcaccggc ctctcacaac tgctgtttcc gggcaaggcc aatggacaac
     2521 tgatcgagcg gaacggcaag ctggttggat cgcggattat cgggcaagcg ttcaccggcc
     2581 ccagctactt ccattcgcgc ccgtccgcag caggaacggg aaatggatac gatcccacat
     2641 ccagcagcgg ctcgaatctg gggcccacca gcaaagtctt gcttgaccgc gtcaatggtg
     2701 atgtgcagaa gttgcacgag gaaaattcgg gctcgccaat ccccgtcgat cttgtcacca
     2761 cgtcgggctc aggcctcgac ccggacgttt ccccggccgc agccgaattc caaatttctc
     2821 gagtcgcacg cgagcgtcac ctgagcgagc ctgaactccg ttcgctcgtt gccaagcaca
     2881 tgttagggag gcagtttggc ttcttaggcg agccgcgggt aagtattcta gagttgaacc
     2941 tggatctcga cgcacaacat ccgctttcga aataatcggt cgactctccg gcgatt
//
LOCUS       DASYVO010000284         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_108730, whole genome shotgun sequence.
ACCESSION   DASYVO010000284 DASYVO010000000
VERSION     DASYVO010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108730"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VGR03_09370"
     CDS             <1..574
                     /locus_tag="VGR03_09370"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEV1994529.1"
                     /translation="ETLEVPPQDVITRDNVSVKVNAVCYFRVVDANRALSQVQNYTYA
                     TSQLAQTTIRSVVGQFELDEILSQRDKVNAKLQMILDTDTEPWGIKVSKVEVKQVDIP
                     EQMQRAIAKQAEAERERRAKIIHAEGEFEASQKLADAAKVLETQPSAIQLRYLQTLTE
                     IGVEKNTTIVFPLPIDIINQWTKNLGGPQK"
     gene            616..1254
                     /locus_tag="VGR03_09375"
     CDS             616..1254
                     /locus_tag="VGR03_09375"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEV1994530.1"
                     /translation="MLESRHVIGLFMLMLLFSGVFFTLGYVMGRNEIDSPVRAATDFF
                     KKPEKSVIPKTDAISKRTSNTAPVTTPTDTAAPPNSDWEFYHAGDNRKTEDHLKPVAS
                     APVPAPARKNLPVLVKTTAPSRTAGGPSKTVSAPLIPGSAYTLQVAALTKEADALELA
                     TRLQKKKFPAFVLSPQADKYYRVQVGPYADPKAADAARKGLEGAGFKAIVKH"
     gene            1254..>2434
                     /gene="lnt"
                     /locus_tag="VGR03_09380"
     CDS             1254..>2434
                     /gene="lnt"
                     /locus_tag="VGR03_09380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEV1994531.1"
                     /translation="MPGGSWLTYLRPQAGAEIPAALRLIVSAVSGAALSLSYTGLYLS
                     IYSWVCVAILLFSVLGARPRVAFGCGFLHGLLFVLTSVPWIATVLTVHGGLSAAGGWG
                     VLLLIAMAWGILIGVFAWTVQRLSRQSIEVACIGAPFIWVTFEFVRAHLPEISFPWNL
                     LGYPASANLGLVQLTAITGIYGLSFLVAGFNALLAWTSASSRLPIRRRITYAAAATTI
                     LSCAALAGPRLVPHAQAHHFARVVQLNFPEVESYSANWFQAHAADLEEIGRISLAPAS
                     EKPDLLVWPEAPAPFSFQDSQFAKIASTLAIRFGHPFLAGAIEWKPTVDPSDVAPHDT
                     FLPYNSALLFDAQGQRVFVYDKVHLVPFGEYEPFPLIHRVVTSVSSDVGGFHKGNKYV
                     VG"
BASE COUNT          501 a          701 c          697 g          535 t
ORIGIN      
        1 tgagacgctc gaggttccgc cacaggacgt catcacgcgc gacaacgtct ctgtgaaggt
       61 caatgcggtt tgctacttcc gtgtggtcga cgccaatcgg gcactttcgc aggtgcagaa
      121 ctacacctat gcgacttcgc agctcgcgca gacgacgatt cgcagcgtcg tcggacagtt
      181 cgaactcgat gaaatcctct cgcagcgcga taaggttaac gccaagctcc agatgatact
      241 cgacaccgac acggaacctt ggggcattaa ggtttccaaa gtcgaggtga aacaggtcga
      301 catccccgag cagatgcagc gcgccatcgc caagcaggcg gaagccgaac gcgagcgccg
      361 cgcaaaaatt attcacgctg aaggagagtt cgaagcctcg cagaaactcg cggatgccgc
      421 gaaagttctg gaaacgcagc cttccgcaat ccagctccgt tacttgcaga ccctgaccga
      481 gataggtgtc gagaagaaca caacgattgt gtttccgctg cctatcgata tcattaacca
      541 gtggactaaa aatctagggg ggccgcaaaa gtaagaatgg cttctggggg aaaacgagga
      601 ggcgccggag agcgtgtgct cgaaagccgg catgtcatcg gcctgttcat gctgatgctg
      661 ctgttttcgg gcgtattttt cacgctcggt tacgtcatgg gacgtaacga aatcgatagc
      721 ccggtgcgcg ccgcgaccga ctttttcaag aagccagaga agtccgttat tcccaagacg
      781 gacgcgattt cgaagcggac gagcaacacc gctcctgtta ccacaccgac ggacacggcg
      841 gcgccgccaa attcggattg ggagttttac cacgcgggcg acaatagaaa aaccgaagac
      901 catctgaagc ccgtggcgtc cgcgccggtg ccagcaccag cgcgaaaaaa cttgccggtg
      961 cttgtgaaga ccacggcgcc atccaggacc gccggcggtc catcgaaaac agtgagcgct
     1021 ccgctcattc cgggcagcgc ttacacgctg caagtggcgg cgctgacgaa agaagcggat
     1081 gcgctggaac tcgcgacgag gttacagaag aaaaaatttc ccgcgttcgt tctttctccg
     1141 caagcagaca aatattaccg tgtgcaagtc ggcccgtacg ctgatccgaa ggccgccgac
     1201 gcggccagga aaggcctcga gggcgcaggt tttaaagcca tcgtgaaaca ctgatgcctg
     1261 ggggcagttg gctcacctat cttcgtccgc aggctggtgc ggaaatacct gcggcgttgc
     1321 gcctcatcgt aagcgccgtg tccggtgccg cgctttcgct ctcctatacc ggtctctatc
     1381 tcagtatcta ttcctgggtc tgcgttgcga tcctcctctt ctcagtattg ggcgcgcgtc
     1441 ctcgcgtggc gtttgggtgt ggatttcttc acggtcttct tttcgtcctg acttcggtgc
     1501 catggattgc gacggttctt actgtccatg gtggcctctc ggcagcgggc ggatggggag
     1561 tcttgctttt gatcgctatg gcctggggaa ttcttattgg cgtatttgcc tggaccgttc
     1621 aacgcttgtc tcgccaaagt atcgaggtgg catgcatcgg cgcaccgttt atctgggtaa
     1681 cgttcgagtt cgttcgcgcg catttgccgg aaattagttt tccttggaac ttgcttggat
     1741 atcccgcgtc cgcgaatctg ggtttagttc agctcaccgc cattacggga atctatgggc
     1801 tatcctttct tgtggcgggt ttcaatgcac tgcttgcatg gacgagcgca tcgagcaggc
     1861 tgccgattcg caggcggatt acatatgcag ccgcagcaac gacaatcctc tcgtgcgccg
     1921 cattggctgg gccgcgcctg gtgccacacg cgcaagctca tcatttcgcg cgtgtcgtgc
     1981 agctcaattt cccggaggtg gaatcgtact ccgcgaattg gtttcaggcg cacgccgcgg
     2041 acctcgagga aatcgggcga atcagcctcg cccctgcctc agaaaagccg gaccttcttg
     2101 tgtggcccga agcgcccgct cctttctcgt ttcaggattc gcaattcgca aagattgcct
     2161 cgacactggc gatccgtttc gggcacccat ttcttgccgg ggccatcgaa tggaaaccaa
     2221 cggtggatcc atcagacgtg gcgccccacg acactttcct cccgtataac agcgcgctgc
     2281 tttttgacgc gcaaggccag cgcgtattcg tctacgacaa agtccatctc gtcccgttcg
     2341 gtgagtacga gccgtttcca ttgatccatc gcgtggtgac cagtgtgtcc agcgacgttg
     2401 gcggcttcca caaagggaac aagtacgtcg tggg
//
LOCUS       DASYVO010000285         4683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_21872, whole genome shotgun sequence.
ACCESSION   DASYVO010000285 DASYVO010000000
VERSION     DASYVO010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21872"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VGR03_09385"
     CDS             complement(<1..685)
                     /locus_tag="VGR03_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1994532.1"
                     /translation="MSFQGKKVLITGSSRGIGRGIALKLAEKGARVAVHYYRNREAAE
                     ETLGKIRKLGSDGFLVQADVCRPEEVRRIFEQVRSEFGSLDIFVSNARTEALTFYEAP
                     MDITLEKWDTAMDSQAKAFLVAVRGAAAMMSKGGRIVAISFAPGGRFGSWQPWVAMGA
                     AKAAMEVLVRYFAVALASRGITVNTISPGWVEDSVLNSLPEAFQTGLREWQEQGWTPM
                     GRLGTPSDIG"
     gene            943..1401
                     /locus_tag="VGR03_09390"
     CDS             943..1401
                     /locus_tag="VGR03_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994533.1"
                     /translation="MSTNRSKGRSGLCSFTFADGRQCRTPRRAGHPHLCAFHAQKEAQ
                     ARAASQAGEDVSYSLSGTYLSACDLTSALGRPFSAVAQGQVKPKTATALAYLGQTLNH
                     SIQLAQHEYINAFGTDAWRKTVRSSFIDPDDAPKPAPQPATPPPKSLASK"
     gene            complement(1925..2503)
                     /locus_tag="VGR03_09395"
     CDS             complement(1925..2503)
                     /locus_tag="VGR03_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994534.1"
                     /translation="MNSRIRLVSIFLSLALLLPALAKASGTVPQGAKISVVTDQAVSS
                     KTAKIGQTVTGTVSQDVTSGGKVVIPKGAAAKLTVSSVQASGRLSTPAKLYLRLRTVT
                     VGGKTYTLATSSAGRTEGGKGKRDAGFIGGGAAGGAIIGALAGGGKGAAIGAAAGAGA
                     GTAVAAATGKKDIEYPAETRLAFTTRTAVAIN"
     gene            complement(2591..3472)
                     /gene="panC"
                     /locus_tag="VGR03_09400"
     CDS             complement(2591..3472)
                     /gene="panC"
                     /locus_tag="VGR03_09400"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEV1994535.1"
                     /translation="METIRTIACMKEQARQARAESRIVGLVPTMGALHRGHLSLIERA
                     RRECSPVIASIFVNPKQFGPNEDFSKYPRTLESDSEKLQRAGVDSLFAPEPSEIYPDG
                     FSTYVHVEGLSERLEGRSRPGHFRGVTTVVIKLLQIVQPNFAYFGRKDAQQSRLITQM
                     ARDLNLDTEIVVCPPVREPDGLALSSRNIYLNAEERKAATVLYRALDAARSELAAGVR
                     DALQLQAVLHRTLSTERQARVDYAEIVDAESFEPIMRIGKPCYVLLAVFIGKIRLIDN
                     LYVEPKSSCSEELLFHF"
     gene            complement(3536..4045)
                     /locus_tag="VGR03_09405"
     CDS             complement(3536..4045)
                     /locus_tag="VGR03_09405"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV1994536.1"
                     /translation="MTPQEMRTLYDYNAWANHRSLDAASALTTEQFVQPMGSSFGSVR
                     DTLAHIFGAEWVWLERFQGRSPASLPDTTQFKDVASLRERWNEHETRLLGFVRGLTQS
                     DLDRVMEYKTLKFGVYRNPLWESMQHLVNHGTYHRGQVTTLLRQLGGQPIATDLMHFY
                     RECATAAGA"
     gene            complement(4084..>4683)
                     /gene="panB"
                     /locus_tag="VGR03_09410"
     CDS             complement(4084..>4683)
                     /gene="panB"
                     /locus_tag="VGR03_09410"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEV1994537.1"
                     /translation="ADMPYGSYHTDMAESLHNAIRFVKEARAEAVKVEGGERRLELIS
                     RLTEAEIPVMGHVGLTPQSVNALGGYRVQGKTPDAAEQLIRDARAVEAAGAFAVVLEA
                     VPRELAAQMTRELRIPTIGIGAGPDCDGQILVIHDLLGLTFGQTPKFARQYANVGETI
                     SKAVREYCDDVREGAFPSDAEAYHAPQSTKDRKSIFIDS"
BASE COUNT          924 a         1553 c         1196 g         1010 t
ORIGIN      
        1 tgccgatgtc cgacggtgtt cccaaacgcc ccatgggcgt ccatccctgt tcctgccatt
       61 cgcgcaatcc cgtttggaat gcttccggca agctgttcag tacgctatcc tcgacccagc
      121 ccgggctgat cgtattcaca gtgatgccgc ggctcgccag tgccaccgcg aagtatcgca
      181 ccagcacttc catggctgct ttcgctgcgc ccatcgccac ccatggctgc cagcttccaa
      241 atcggccacc cggcgcgaac gagatggcca caatccttcc gcccttcgac atcatcgcag
      301 ccgccccgcg cactgccacc aggaatgctt tcgcctgcga atccatcgcc gtgtcccact
      361 tctccagcgt gatgtccatc ggcgcctcat aaaacgtcag cgcttctgtt cgcgcgttgc
      421 tgacgaaaat atcgagcgac ccgaactccg accggacttg ctcgaagatc cggcggacct
      481 cctccggccg gcaaacgtcg gcctgtacca ggaatccgtc cgaaccaagt ttgcggattt
      541 ttcctaacgt ctcctccgcc gcttctcggt tccgatagta atgaaccgcg acgcgcgctc
      601 ctttctccgc cagtttcagc gcgatgcccc ggccaatgcc cctggaactc ccagtgatca
      661 aaaccttttt tccttggaat gacatagaga cctccatgac tgccgcgccg catgttagtg
      721 ctccacttcg ccatcgtcaa caccgcgcca tgcgcctctc ctcccctcat tccaggccct
      781 gtaaatataa gaaaagaagc tgttttagaa aggtgtgata ctcggtttct tttttatgga
      841 caatccggtc acgactctgg tatattatcc ttgttcagta gtgcgcctcg ccttccgcga
      901 gactcccctt acaactcaaa tctttcattt ctggagactt ccatgtccac taaccgctcc
      961 aagggccgtt ccgggttgtg ttctttcacc tttgccgacg gccgccagtg ccgcactccg
     1021 cgccgcgccg gccacccgca cctctgcgcg ttccacgcgc aaaaggaagc gcaagcccgc
     1081 gccgcctcac aggccggaga ggacgtatct tattccctct caggcactta tctctccgcc
     1141 tgcgatctca cttccgcttt gggccgcccc ttctccgccg tcgcccaggg ccaagtcaaa
     1201 cccaaaaccg ccaccgccct cgcctacctc ggccagactc tcaatcattc tatccagctc
     1261 gcccagcacg aatatatcaa cgccttcggc acggatgctt ggcgcaagac cgtgcgctcc
     1321 tccttcatcg atcccgacga tgccccgaag ccagctcccc aaccggccac gcccccaccc
     1381 aaatccctcg cctccaagta gcgcagaatt cattctgtgc tcgttttccc gccgttgtag
     1441 gggccgccgt ctgcggcggg ctgctcttct ccctccgtgc tcctggtaac gatcaagcgg
     1501 gcaaaaccgc actttttgta ctaacttatt tagaatcaac acttgcaaaa atgtgccaaa
     1561 aacacagctc taaccacctt tagaatcaac acttataaaa gtgttaactc taaaggacct
     1621 taactctctt tagaatcaac actcatgaaa aacatagggg gtagggaggg gtgttatggt
     1681 taaccagaca cccaatgaag ggtgtctgtc ctgaacgacc atcgggaagt gatcgcagat
     1741 tccggacctg ccggaaagga tctctcctcc cacgcgacaa agcatgtcta tcccgagcga
     1801 ccgtcggaaa ccgaggaacc tctctcgaaa tctacggcca tccactccaa ctgatcgaga
     1861 ctagcaccgg cttgcgcaga gggccgcgta gcttcgcgcg gccctctgcg ccctgtgatt
     1921 cgcactaatt gatcgctacg gccgtccttg tggtgaacgc tagccgcgtc tccgcggggt
     1981 actcgatatc tttcttcccc gtcgccgccg ctacagctgt tccggctccc gcgccagccg
     2041 ctgcaccgat cgctgcgccc ttgccgcctc cagccagcgc tccaataatc gcgccgccgg
     2101 ccgctccgcc gccgatgaac ccggcatccc gtttgccctt gccgccctcg gttcttccgg
     2161 cggagctcgt cgcgagcgtg tacgtcttgc cgcccacggt cactgtgcgc aacctcaggt
     2221 atagttttgc cggcgtggac aaccggcccg aggcctggac gctgctcacc gtcaatttcg
     2281 ccgctgcgcc cttgggaatc accactttgc cgcccgacgt cacatcttgc gagaccgttc
     2341 ctgttacggt ctggccgatc ttggccgtct tggaactcac cgcctggtcg gtcactacag
     2401 aaattttcgc tccttgtgga accgtgccgc tcgctttcgc tagcgcaggc aatagcagtg
     2461 ctagcgaaag aaaaatcgaa accaatcgaa ttcgagaatt catctccgtc acctcaataa
     2521 agaatttggg gctgtaggga aagtctagca aaagaagccg ttagaagggg atgagcgcgc
     2581 cccaacagtg ctagaagtga aacagcagtt cttcggaaca cgatgatttc ggttctacat
     2641 acaagttatc gatcaagcgg atctttccga tgaaaacagc gaggagcacg tagcagggtt
     2701 tgcctatgcg catgatcggc tcgaacgatt cggcatccac gatctccgca taatccaccc
     2761 tcgcttggcg ttccgtgctg agggtgcggt ggagcacagc ttgcaattgc agcgcatcgc
     2821 gaactccggc ggcaagctcg cttctcgccg catccagcgc gcgatacaaa acggtggccg
     2881 ccttgcgttc ttccgcgttc aaataaatgt ttcgggagga aagggccaag ccgtcgggtt
     2941 cgcggaccgg cgggcaaacc acgatttccg tatccagatt caagtcgcgc gccatttgtg
     3001 tgatgaggcg cgattgctgc gcatccttgc gcccgaagta cgcaaagttc ggctgcacaa
     3061 tttgtagcag ttttatcact acggtcgtga cgccacgaaa gtgccccggc cgcgagcgtc
     3121 cttcgagccg ctcgctcaac ccttcgacat gaacgtaggt cgagaatcca tcaggataga
     3181 tctccgacgg ttccggcgca aaaagcgaat ccacgcccgc gcgctgaagt ttttcgctgt
     3241 cgctctcaag cgtgcgcgga tattttgaga aatcctcgtt ggggccaaac tgcttcggat
     3301 tgacgaagat ggaagcaatg accggcgagc actcccgcct ggctcgctcg atcagcgaca
     3361 aatgcccacg gtggagcgcc cccatcgtcg gcaccagtcc gacgatgcgg ctttccgcac
     3421 gcgcttgccg cgcctgctcc ttcatacacg caatcgttcg gattgtttcc aaggtgtggt
     3481 agcgccggta tcttgccggc tctcattatc tcgaaattgg aatggttctc acaaactacg
     3541 cgcccgccgc cgtggcgcat tcgcgataga agtgcatcag gtctgtcgcg atgggctgcc
     3601 caccaagctg ccgcagcagc gtggtgacct gtccgcggtg atacgttccg tgattgacca
     3661 gatgctgcat cgattcccaa agaggattcc tgtatacacc gaacttcagc gtcttgtatt
     3721 ccatcacgcg gtccaagtcg gattgcgtca gtccgcgcac aaaaccaagc aggcgcgttt
     3781 cgtgttcgtt ccagcgctcc cgcaagctcg ccacgtcttt gaattgcgta gtatccggga
     3841 gcgatgcagg cgagcggccc tggaagcgtt ccagccagac ccactccgcg ccgaaaatgt
     3901 gcgcgagcgt atcgcgaacg gagccgaagc tcgagcccat cggctgaacg aactgctccg
     3961 tcgtgagagc cgaagccgcg tccagcgacc ggtgattggc ccaggcattg tagtcataca
     4021 gcgtgcgcat ctcttggggc gtcatgtcag ggtcctcgtt gcgttttaag atttgcgggc
     4081 tgctcagctg tcgatgaaaa tgctcttgcg atctttcgtg gattgcggcg cgtgatacgc
     4141 ctcggcatcc gaaggaaatg cgccctcgcg cacatcatcg cagtattcgc gtacggcttt
     4201 ggaaatagtc tctccgacgt tggcatactg gcgcgcaaat ttcggcgtct ggccaaacgt
     4261 aagtcccaga agatcgtgaa tgacgagaat ttgcccgtcg cagtccgggc cggcgccgat
     4321 gccgatcgtc ggaatgcgca attcgcgcgt catctgcgcc gccagctcgc gaggcactgc
     4381 ctccagaaca accgcaaaag cccccgccgc ttccaccgcg cgcgcatcgc gaatcaactg
     4441 ctcggcagcg tccggcgttt ttccctgcac gcggtatccg cccagtgcat tcaccgattg
     4501 cggcgtcaag ccgacgtgtc ccatcaccgg gatttccgct tccgtcagcc gcgaaatcaa
     4561 ttccagacgg cgctccccgc cctccacctt tacggcctcg gcgcgcgctt cttttacgaa
     4621 acgtatggca ttgtgcagcg actccgccat gtccgtgtga tagctgccgt aaggcatgtc
     4681 cgc
//
LOCUS       DASYVO010000286         3149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_188793, whole genome shotgun sequence.
ACCESSION   DASYVO010000286 DASYVO010000000
VERSION     DASYVO010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188793"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VGR03_09415"
     CDS             <1..183
                     /locus_tag="VGR03_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994538.1"
                     /translation="YAPGGRVSEVDRSGGGQTSGHAGRVEQNLHTGGTAGWSACGVLG
                     VTGGRRSWPGLRSAVT"
     gene            548..772
                     /locus_tag="VGR03_09420"
     CDS             548..772
                     /locus_tag="VGR03_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994539.1"
                     /translation="MAAMQTVEDAAVEFERDRTEVHVLSADLTGISVALIWNFPIALG
                     AVAIQITLAYAIMLRLLERLAYGSPGGSGR"
     gene            900..1487
                     /locus_tag="VGR03_09425"
     CDS             900..1487
                     /locus_tag="VGR03_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994540.1"
                     /translation="MRVKTIQLSLAIPILLFAFAGCEVNDNGPKISAGSGKQSGWRAH
                     CARELGFLIYYPPEMELKVEPSKITAEEQFNLSEWRVPGSEWFVVLHVHESPPEGRTP
                     SLREWLQDLGGDQAEATLAGDIPAVLTTSLFEAVFEKSVFFVEKSSRRLVEIRLIVPN
                     IADWMGPAERVAPQYRSQERIFDYMVDSIHVGKCP"
     gene            1638..1844
                     /locus_tag="VGR03_09430"
     CDS             1638..1844
                     /locus_tag="VGR03_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994541.1"
                     /translation="MRDEKENHKEERGEPGGQSVPGHQPAEDPSDERAVTQEENVRHS
                     LRKSGCPGQQDANGGKEIGDSLQG"
     gene            complement(2456..3043)
                     /locus_tag="VGR03_09435"
     CDS             complement(2456..3043)
                     /locus_tag="VGR03_09435"
                     /inference="COORDINATES: protein motif:HMM:NF022216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEV1994542.1"
                     /translation="MSPESSQWIGVGIAPIAVMVVLSFLLKNRRERARREGGSGVLVL
                     EYGRAVKSVPILVAVAWTILIPIVLHDDPIRRENLPAFLLLVVLITVPLVVVTIEAFG
                     VAHRIREDGIEKRSPWSSHRMIKWEDIREISFSGAMQWFVVSNPHGKLRLHLYLNGLK
                     DFADAVRAKVPKETWAKAEAHLKNLDAKRPSPDTE"
BASE COUNT          637 a          937 c          953 g          622 t
ORIGIN      
        1 tacgctcccg gcgggcgtgt ctctgaggtg gatcgttccg ggggggggca gacctcggga
       61 cacgcggggc gagtcgagca gaatctccac accggcggaa cggccggctg gagcgcgtgc
      121 ggcgtgcttg gtgtcacagg cgggaggaga agttggcccg gtttaaggtc cgcggtgacg
      181 taaagctcct tcctcgggcg gcctgtccgg atagggggaa agcatagagc tacaagcacc
      241 gagcctgagg gattgtccgc gcctttggcg gtccaagggg ggtggagccc gacctccccg
      301 cacgccttgg cgtggaattt tcggcgccgc cgctttccta ctgctcgccc ccgcatcggg
      361 cctcgcggcc gatcgaacgg atcgctgcgc cgccgcgtgt tacgagctcg cccaggccgg
      421 tccggctccg acggcgccac cagccaacag cagcctgacg ccagcgctcg tcagtccccg
      481 cctgcctgct agtcccgcgc cgccgcgtcc tgctgctgcg gcccctgtgc cgccgccgga
      541 gtggtatgtg gcggcgatgc aaaccgtcga ggacgccgcc gtcgagttcg agcgcgatcg
      601 cactgaggtg cacgtcctca gcgcggacct caccggcatc agcgtcgccc tcatctggaa
      661 ttttcccatc gccctcggtg cagtcgccat ccagattacg ctcgcctatg ctatcatgct
      721 gcggctgctg gagcggctcg cctacggaag tccgggaggg tcgggtcgct aagtcgaaat
      781 cttatggtgg cgcgtttcgg tgatctaccg gaggcgattc aagctatgct cgcgccgttc
      841 cggttggcgg ccatcgagtt tagcaaagca gaggtcatcc gcatcgacga gcacttcgag
      901 tgcgagtaaa aacgatacaa ctcagtcttg cgatcccaat tctgctgttc gcttttgccg
      961 gatgcgaggt gaacgacaat gggccgaaga tttctgccgg ctcaggaaaa cagtcgggtt
     1021 ggagagcgca ttgcgctcgg gaacttggct tcctcatcta ttatcctcct gaaatggaac
     1081 tgaaggtcga gccttcgaaa ataaccgctg aggaacaatt caatctatcg gaatggagag
     1141 ttcctgggtc agaatggttt gtcgtccttc acgtgcatga atcccctccg gaggggagaa
     1201 cgccgtcgct tagggaatgg ttgcaagact tgggtgggga tcaagcagag gcgaccctgg
     1261 ctggcgatat cccggcggtc ttgacgacgt ccctctttga ggcggtcttt gaaaagagcg
     1321 ttttcttcgt cgagaagtcg agtcgacgac ttgtggaaat aaggctcatt gttcctaaca
     1381 tcgcggactg gatgggccca gcggagagag tcgcgccgca ataccgcagc caggagcgga
     1441 tatttgatta catggtcgat tcaatacatg tcggcaagtg tccatgagcg ggcgttttct
     1501 gacctttctt tgctctggca gtgtgcagca ggcggtgagc cgtgcccaat accagtggcg
     1561 atcaccagcc caaaaggcct tcacaagggc gcgcagcact tcttcgatat ccctgccagc
     1621 ttcaactgag ttccgctatg cgcgacgaga aggaaaatca taaagaggag cgcggcgagc
     1681 cgggagggca aagtgtgccg ggtcatcaac cggccgagga tccctccgat gagcgcgccg
     1741 taacacaaga agaaaacgta agacacagcc tgcggaagtc cggctgtcca ggccagcagg
     1801 acgcgaacgg cggcaaagaa attggtgaca gcctccaggg ctgactccgg ccattgttga
     1861 cccaggcggc agcgaccacc gccaccgaaa ccaccaggtc cgcaacggcc ccgataatga
     1921 gcctacgcgg gctgccactt gccatagcac atgagggcat tacctctgtt gagtcctgtt
     1981 cctccactgc gccatgagat cggccagaga ctccgcgctc attccatacg tatcgggaag
     2041 gctctcgtcg actccgctta cggccttgtt cagcttccgc accgcgccct cggtgtaccg
     2101 aagccttccc tgcgccgtca gcgtgaagat aacccgcgtc gttcccttac tgtaatgctc
     2161 agcgtgctga actgctctac ttcttcccag cgaaaattct tcgatccaaa cagcgtccgc
     2221 aaagttaagc cggtcggcgt cagcacaagg tacgcgcggt cagggagcag attcatgaaa
     2281 gacagaaccg cccccgcgag gaagaagacg accaatgagc ccacgacgag caggctgtcg
     2341 tcgggtgggt cacgtcttgg agtgtaagcg tacacagtgc aaagccgcct gcgagcagaa
     2401 tgaacaggac gcccagcaaa atccacttcc tccggctggg cgtgagttta agagtctatt
     2461 ccgtatccgg gctcggacgc ttggcatcaa gatttttgag gtgagcttct gccttggccc
     2521 atgtttcttt tggaactttc gcccgcactg cgtcggcaaa atccttcaag ccattgaggt
     2581 agaggtgaag cctcagtttt ccgtggggat tcgataccac gaaccactgc atcgccccgc
     2641 tgaacgagat ttcacgaata tcctcccatt taatcatgcg atggctcgac caaggggagc
     2701 gtttctcgat cccatcttct ctgatccggt gggcgacgcc gaaggcctcg atggttacga
     2761 cgacaagggg cacggttatc agcacaacca aaagcaaaaa ggccggtagg ttctctcttc
     2821 ggatcggatc gtcgtggagg acgatcggaa tgaggatcgt ccacgcgact gcaacgagaa
     2881 tgggaacgct cttaaccgct cggccgtatt caaggaccag caccccggag ccgccctccc
     2941 gccgcgctct ttccctgcgg ttcttcaaga gaaaactcag gacgaccatc acggcgatcg
     3001 gcgcgatgcc gacaccgatc cattgcgagc tctccggact catgtgtcct ccgatggaat
     3061 aaggtttcca acccgcgctg tggcagcggc tcctggggtg gccggtgtct gagtatgggc
     3121 gattatgtca cggggccggg ggccacgag
//
LOCUS       DASYVO010000287         5901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_231735, whole genome shotgun sequence.
ACCESSION   DASYVO010000287 DASYVO010000000
VERSION     DASYVO010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231735"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VGR03_09440"
     CDS             <1..367
                     /locus_tag="VGR03_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEV1994543.1"
                     /translation="AIYAGEVRQFAAGGAQLLINISNDGWFGRSAAAEQHLRMARVRA
                     VENRRWIVRSTNNGFTVSVDPYGRIFQPLPPDVRAAADLPYDFRSDETIYTRFGDWFA
                     WLCVLISVILVATTFRKAK"
     gene            501..1562
                     /gene="prfB"
                     /locus_tag="VGR03_09445"
     CDS             501..1562
                     /gene="prfB"
                     /locus_tag="VGR03_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV1994544.1"
                     /translation="MKSLEARVADPNFWNNQEAAQSVLRERKRAEEQIDADEKLTSIS
                     SDIETYIHLAHEETNVEQKNDLLKDLDRELNAADIYISELETKTLLSGETDHLNAIMT
                     IKPGAGGTESQDWASILLRMYLRWAERRGMTASVLDETPGEEAGIKSATIQFSGENAY
                     GLLTGETGVHRLVRISPFDQAARRHTSFASVFVIPEIDDRIEINIRPEDLRVDTFRSG
                     GKGGQNVNKVETAVRITHIPTNIVVGCQADRSQHRNRELAMKMLRSRLYDLEIKKRQA
                     HTDKLDESKLDISFGSQIRSYVMQPYRLIKDHRTKFEVGDVDRVMDGDLDPFIRSYLM
                     AKKTKGRLDIQPDDDADLA"
     gene            1584..2954
                     /locus_tag="VGR03_09450"
     CDS             1584..2954
                     /locus_tag="VGR03_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEV1994545.1"
                     /translation="MRWTKRVRFLSFAVRLALLSLALAVSAFAVEKSTAPQLIELAKS
                     NSAGLRDAITATFDGKDLKEGTAWVGRGPDFFFATEAPSQPSLVIDGAPGPQVRQLAG
                     SSLWYAPARIEQVGRLHAFYYLINGTKFGGRLDLPAFGPSSYLQPGVPSGTLSPKIAH
                     TSKIYDGMRSEYWIYVPAQYHQDTPAALMVFQDGGWYIDRNGNNPALNVIDNLIAQKK
                     IPVMICVFVNPGDISDSPGTPTYRFVKAYSDKWQRTLKDSMRSTLYDSVSDRYARFLR
                     DELLAEVAAKYNIRKDAYSRAITGLSSGGICAFNAAWQMPDQFSRVISWIGSFTSIQW
                     REDPNAQDGGQDYPEKVLRESKHNIRVWVQDGSEDIESDRYGSWPLANLRLANALKFK
                     DYDFHFSFGKGTHNSAQGAAEFPAEMIWLWREYDPAKTAQTYEMEPSEKAKPLFRVSV
                     ANRDTE"
     gene            3281..4471
                     /gene="metK"
                     /locus_tag="VGR03_09455"
     CDS             3281..4471
                     /gene="metK"
                     /locus_tag="VGR03_09455"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV1994546.1"
                     /translation="MSEALRNFLFTSESVTEGHPDKIADQISDAVLDEVMKHDPKGRV
                     ACETLVTTGVAVVAGEITTTAHVDYDELVRSTIRGVGYDRAKYGYDADTCAVMCTVKR
                     QSPDIAMGVDTGGAGDQGLMFGFACDETEELMPMPIQLAHRLTQRLAEIRKANRVDFL
                     RPDGKSQVTIEYRNGRPARVDCVVISTQHSDKVSNTDLRDAITEHVIKPIIPASMLDA
                     KTKYHINPTGRFVIGGPLGDAGLTGRKIIVDTYGGYSRHGGGAFSGKDPSKVDRSACY
                     MARYIAKNIVAAGLARKTEVQLAYAIGVAEPVSVMAETFGTATMPEEKITALIREFFA
                     LTPKAIIEALDLRRPIYKATAAYGHFGRSGAGFTWERTDRADALRKAAGLGAAATAEV
                     GARR"
     gene            4575..5849
                     /gene="ahcY"
                     /locus_tag="VGR03_09460"
     CDS             4575..5849
                     /gene="ahcY"
                     /locus_tag="VGR03_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEV1994547.1"
                     /translation="MAIAELKKGDVKDMGLADAGKRKIEWAGQQMPVLESIRKRFVKE
                     QPLKGLRVSACLHVTSETANLMIALRDGGADAVLCASNPLSTQDEVAASLNRDYNIPT
                     FAIKGEDNNTYYSHLSAALEHKPQFTMDDGADLVTMLLTKRTELVPNVIAGTEETTTG
                     VIRLRAMAKDGTLKYPIIAVNDAMTKHFFDNRYGTGQSTIDGVIRATNVLLAGLKVVI
                     AGYGWCGRGVAMRAKGLGADVIVTEIDPVKGIEAAMDGFRVMPMAEAVKEGDVYITVT
                     GNKSVIRGEHFEKMKGGAIVCNSGHFNVEIDIPALERMSSGKREVRPMVDEYQMKDGR
                     KVYLLAEGRLVNLSTAEGHPASVMDMSFANQALSAEYLRANYKTLKPQVYVVPEHLDK
                     EIARLKLDSMGHRLDKLTADQEHYLASWQEGT"
BASE COUNT         1281 a         1754 c         1703 g         1163 t
ORIGIN      
        1 agcgatctat gccggcgaag tgcgccagtt tgccgcgggt ggggcgcagc tcctcatcaa
       61 catttcgaat gacggatggt ttgggcggtc cgctgccgcc gagcaacatc tgcgcatggc
      121 ccgcgtgcgt gccgtggaaa accgccgctg gatcgtgcgc tcaacgaata acggctttac
      181 cgtttcggtc gatccctacg gccgcatctt ccagccgctt ccgccggacg tgcgggccgc
      241 agccgatctt ccttatgatt ttcgatcgga cgagacgatt tacacgcgct ttggggactg
      301 gttcgcctgg ctctgcgtac tcatttccgt tattctagtg gcgacaactt ttcgaaaggc
      361 aaagtgacgt ccgttcccgg acggacgatc tacctgaaaa agcatgatcc tcgaagacct
      421 ccagtaccgc tacgaagggc tcaagcagcg catcacgctc gtgcggagct atctttgacg
      481 cgccgcagta ccgcgagcgt ctgaaatctc tcgaagcgcg cgtcgccgac cccaattttt
      541 ggaacaatca ggaagccgca caaagcgttc tccgcgagcg gaagcgcgcc gaagagcaga
      601 ttgacgcgga cgagaagctc acgtccatct ccagcgacat cgaaacgtac atccatctcg
      661 ctcacgaaga gaccaacgtc gagcaaaaga acgatctgct gaaagatctg gaccgcgagc
      721 tcaacgccgc cgatatctac atctccgaac tggaaaccaa gacgctgctc tccggtgaga
      781 ccgatcacct caacgccatc atgaccatca agcccggcgc gggcgggacg gaatcgcagg
      841 attgggcttc catccttctt cgcatgtacc tgcgatgggc ggagcgccga ggaatgacag
      901 cctcggtgct cgatgagact ccgggagaag aggcgggcat caaatccgcg acgattcaat
      961 tttccggcga aaatgcttac ggccttctca cgggagaaac cggggttcac cgcctcgtcc
     1021 gaatctctcc cttcgatcag gccgcgcggc gtcacacttc ttttgcgtcc gtgttcgtga
     1081 ttccggaaat cgacgatcgc attgagatca atatccggcc cgaagacctg cgcgtggaca
     1141 cgttccgctc gggtggcaag ggcgggcaaa acgttaacaa agtggagacc gctgtccgta
     1201 tcacccacat cccgaccaat atcgtcgttg gatgtcaggc cgatcgcagc cagcacagaa
     1261 atcgcgaatt ggcgatgaag atgcttcgct cgcggctcta cgacctcgag atcaagaagc
     1321 gccaggcgca caccgacaaa ctcgatgaga gcaagctgga catatccttt ggcagccaaa
     1381 tccggtctta tgtcatgcag ccgtaccggc tgatcaagga ccaccgcacg aaattcgaag
     1441 tgggcgatgt ggaccgcgtg atggacggcg acctggaccc cttcatccgc tcctacctga
     1501 tggccaaaaa gacgaagggc aggctcgaca tccagccgga cgacgatgca gacttggcct
     1561 aatcaacacg aaggagtcct tggatgcgct ggacaaaacg ggttcggttc ttgagttttg
     1621 cagtgagact tgcgcttctc tcattggccc ttgccgtctc cgcgttcgct gtagaaaaat
     1681 cgaccgcacc gcaattgatc gagcttgcca agtccaacag tgcgggcttg cgggacgcca
     1741 tcacagctac ctttgatggc aaggacctta aagaagggac cgcgtgggtc ggccgtggcc
     1801 cggacttctt ctttgccacc gaagcgcctt cgcagccatc gctcgtcatt gatggcgctc
     1861 cggggccgca ggtacggcag ttagcgggct cctctctctg gtatgcccct gcgcgcatag
     1921 agcaggtggg ccgcttgcac gcgttctact acctcatcaa tggaacaaag ttcggtggcc
     1981 gcttggattt gccggcgttc ggcccttctt cctatctgca accaggtgtt ccctcgggca
     2041 cgctgtcgcc gaagatcgcg cacaccagca agatctacga cggcatgagg agcgaatatt
     2101 ggatttacgt tcctgcgcaa taccatcagg acacgcctgc cgcgctgatg gtcttccagg
     2161 acggcggctg gtacatcgat cgcaatggca acaatccggc gctcaacgtc atcgataact
     2221 tgatcgctca aaaaaagatt cccgtgatga tctgcgtctt cgtcaatccg ggggacatta
     2281 gcgactcccc gggcacgccg acctaccgtt ttgtgaaggc ctattcggac aagtggcagc
     2341 gcaccctcaa ggattccatg cgcagcacgc tatatgacag cgtgagcgac cgctacgctc
     2401 gctttctccg cgatgagctg ctcgccgagg tcgctgccaa atacaatatc cgcaaggatg
     2461 cctacagccg cgcgatcacc ggtctctcct cgggcggcat ctgcgccttc aacgcggcat
     2521 ggcagatgcc cgatcagttc agcagggtga tttcgtggat cggcagcttc accagcatcc
     2581 agtggaggga agatccaaat gcgcaagatg gtggacagga ctaccccgaa aaagtcttgc
     2641 gcgagtccaa gcacaacatt cgcgtttggg tgcaggacgg ttccgaggat atcgaatcgg
     2701 accgttacgg tagctggcca ctggccaatc ttcggcttgc caacgcactg aagtttaagg
     2761 attacgactt ccattttagt ttcggcaagg gcacacataa ctcagcccaa ggcgcggccg
     2821 agttcccggc ggagatgatc tggctctggc gggagtacga ccctgccaag accgcgcaga
     2881 cctacgagat ggaaccgtct gagaaggcta agccgctatt ccgagtctcg gttgcgaacc
     2941 gtgacaccga atgaggcaag aactggattt gattcagtcc ctgtcaagag tcgctctcag
     3001 gagtcgccac gctaacggat gggcctgcag ttcttttctt cgatttgggc atgagagcgc
     3061 gctcggcgag cctggacgct tggcaggtgg ctccccaaga cagccctaat cttccattcg
     3121 agcctcgatt tcgaccgttc gacccgctcc taaggcattt ggactcaata aattgattct
     3181 cgcgcaagcc ggtgatatct ttgaaaggtt acgcacacag tgttttgcag cgcagttggg
     3241 ggggatgctt cccaacgctg cctatgaagg agagtttgtt ttgagcgaag ccttacgcaa
     3301 cttcttgttt acctcggagt ccgtgaccga aggccatccg gacaaaattg ccgaccagat
     3361 ttctgacgcc gtccttgacg aggtcatgaa gcacgacccg aaaggacgtg tcgcttgcga
     3421 aacgcttgtc acgacgggtg tggcagttgt cgctggtgaa atcacgacca ccgcgcacgt
     3481 cgattacgac gagcttgtac gcagcaccat ccgcggcgtc ggctacgatc gcgcgaagta
     3541 tggttacgac gcggacacct gcgccgtcat gtgcacggtg aagcggcagt caccggacat
     3601 cgccatgggc gtggataccg gcggagcggg cgaccagggc ttgatgtttg ggttcgcctg
     3661 cgatgaaaca gaagaattga tgcccatgcc tatccagctc gcccaccgcc tcacgcagcg
     3721 cctcgccgag attcgcaaag ccaacagagt tgattttctc cgtcctgatg gaaaatctca
     3781 ggtcaccatc gagtatcgca acggccgccc ggcgcgcgtc gattgtgtgg tcatctcaac
     3841 ccagcacagc gacaaggttt ccaacacaga tctccgggac gccatcaccg agcacgtgat
     3901 taagccgatt attcccgcct ccatgctgga cgccaagacg aagtaccaca ttaatcccac
     3961 gggacgcttt gtaattggcg gtcccttggg cgacgcgggc ctcaccggcc gcaagattat
     4021 cgtcgatacc tatggtggtt acagccggca tggtggcggc gcgttctccg gtaaggatcc
     4081 ctccaaggtg gatcgctcgg cttgctacat ggcgcgttac atcgcgaaga acattgtggc
     4141 tgcgggcctg gcgcgaaaga cggaggtgca attggcttac gccatcggcg ttgccgagcc
     4201 tgtctcggtc atggcggaga ctttcggcac agccacaatg ccagaagaaa aaatcaccgc
     4261 gctcatccgt gaattttttg cgctgacgcc gaaggccatc attgaagcgc tcgacctgcg
     4321 ccgtccgatt tacaaggcca ccgccgctta tggccacttc ggccgcagcg gagctgggtt
     4381 cacttgggag cgcacggacc gcgcggatgc tctccgcaag gccgctggcc tcggggccgc
     4441 cgcgactgcg gaagttggag cgcggcgcta acactcgaaa gttgattcgt accggcccgc
     4501 ctttccccgg gcctattccc gagtgctcct ttgcactccg ttcatgaacg aaatccaaat
     4561 tgaggagcat gacattggca atcgctgaac tgaaaaaagg tgacgtgaag gacatgggac
     4621 tggccgatgc cggcaagcgc aaaatcgaat gggcggggca gcaaatgccc gttctcgaat
     4681 cgatccgcaa gcgcttcgtc aaggagcaac cgctcaaggg cctgcgcgtt tccgcctgtc
     4741 tgcacgtgac cagcgaaact gcaaacctga tgatcgcgtt gcgcgacggc ggcgccgatg
     4801 ccgtgctgtg cgcctccaat ccactatcca cgcaggacga agttgccgcc tcgctgaatc
     4861 gcgattacaa cattccgacc ttcgccatca aaggcgagga taacaacacc tactattcgc
     4921 acttgagcgc ggcgctggag cacaagccgc agttcaccat ggacgacggc gcggacctgg
     4981 tcaccatgct gttgaccaag cgtaccgagc tcgtcccgaa cgtaatcgcc ggaacggaag
     5041 agaccaccac cggtgtgatc cgcctgcgcg cgatggccaa ggatggcacg ctgaagtatc
     5101 cgatcatcgc cgtgaatgat gcgatgacca agcatttctt tgacaaccgt tacggcacgg
     5161 ggcagtccac gattgacggc gtcattcgcg ccactaatgt gctactcgcc ggcctgaagg
     5221 tagtgattgc cggttacggc tggtgcgggc gcggcgtggc catgcgcgcc aagggcctgg
     5281 gcgcggacgt gatcgtcacg gagatcgatc cagtaaaggg catcgaagcg gcgatggacg
     5341 gcttccgagt gatgcccatg gccgaagcgg tgaaggaagg cgacgtttac atcaccgtga
     5401 cgggaaacaa gagcgtcatc cgcggcgagc actttgaaaa gatgaagggc ggagcgattg
     5461 tttgcaactc cggccacttc aacgtggaga ttgacatccc ggcgctggaa aggatgtcca
     5521 gcggcaagcg cgaagtgcgc ccgatggtcg acgaatatca gatgaaggac ggccgcaagg
     5581 tctacctgct cgccgaagga cgccttgtca atctttccac tgccgaaggg catcccgcat
     5641 cggtgatgga catgagcttc gccaaccagg cgctttccgc cgaatacctg cgtgcgaatt
     5701 acaaaaccct gaagccgcag gtgtatgtgg tgccggagca tctggacaag gaaatcgcgc
     5761 gtctcaagct cgattcgatg ggtcacagac tcgataagct cacggcggat caggaacact
     5821 acctcgcttc ctggcaggaa ggcacctaac tgtcgccgcc attcccggct gcttgatcgt
     5881 gttatctttg caggggcgcc c
//
LOCUS       DASYVO010000288         8383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_77176, whole genome shotgun sequence.
ACCESSION   DASYVO010000288 DASYVO010000000
VERSION     DASYVO010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8383
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77176"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /locus_tag="VGR03_09465"
     CDS             <1..1531
                     /locus_tag="VGR03_09465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEV1994548.1"
                     /translation="IAVTVTLATFMEVLDTSIANVALPHIAGNLSAGSDESTWVLTSY
                     LVSNAIVLPLSGWFSGVIGRKKFYMACVAIFTVSSFLCGLAPSLGVLVIFRILQGVGG
                     GGLQPSEQAILNDTFPLEKRGMAFAVYGLAVVVAPTIGPWLGGWITDNFSWRWIFYIN
                     VPVGMLSLLLTNFLVSDPPYMKKSSLKAGFRIDYIGIGLISLGLGSMQIILDKGQRED
                     WFSSKFIVVLFLLMLIGIVAGILWELREKEPVVDLRMLKDRNFAVATVAMFFLGFVLY
                     ASTVLIPQFLQQMLGYSAQLAGMALSPGGAVIMCMMPVVGILVSKVDTRILITFGCIV
                     SASALFVMAGWDLGLDYGHAVRARMLQSFGLAFLFIPINVAAFAYVPKEKTNMGTGII
                     NLARNIGASVGIATVTTMLDRGAQFHQARLTEHVNDFSAAYHNMLNGTQAKLIAAGST
                     AAHASAQAHEMIYHTIQRQAVMLAFIDNFKMLGVVFFAVIPILALMRKPKMRAGAVPV
                     H"
     gene            1591..2241
                     /locus_tag="VGR03_09470"
     CDS             1591..2241
                     /locus_tag="VGR03_09470"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1994549.1"
                     /translation="MATQAAVGIQLNEEKLIRAGQRGDEQAVEALFRRYQRPLFQTAL
                     RVLGNAEDAEDALQDGLLSAYRNLKRFEGRSQFSTWLTRIVINAALMRRRSAKARPAV
                     SLDAPPRENELPAAERFADDGPNPEQVFASTEIREMISENLDELSPLLRTAFVLREVQ
                     GYSTGEAAKKLGVTENTLKARLWRARHQLAERLGRRLRRMKDDIAGGMGDAECSYC"
     gene            complement(2345..3196)
                     /locus_tag="VGR03_09475"
     CDS             complement(2345..3196)
                     /locus_tag="VGR03_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1994550.1"
                     /translation="MSTSDRKTMRVAFQGEPGAFSEAAAIQLLGDAITTVPRATFDAA
                     FRAIDEVAADAFLAPVENTLAGSVVRVYDLLLESHLAIVAETILPIEHHLIGVPGATL
                     AGLRSVASHPMALAQCERFFSSHPELKRVPAEDTAGSVREVLARGDKSSAAIAGRRAA
                     EHYKGVILSESIQDNAENFTRFVLLLPENEAGAWLSPGARKVSLAMRLAHRPGALLAS
                     LEPFAKHAVNLLKIESRPIHGRPWEYQFFIDVEATDVARLDQALTEVRSATSELRVLG
                     RYAAASR"
     gene            3463..3990
                     /locus_tag="VGR03_09480"
     CDS             3463..3990
                     /locus_tag="VGR03_09480"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV1994551.1"
                     /translation="MNRPEATEYADYYANYIAKVPGSDVLSVLESQRLQMLQLFAGRS
                     ERDGSFRYAPGKWTVKEVLGHITDTERIFTYRALRFARADQTALPGFEQDDYVRSGAF
                     GDRTLAGLAEEFGAVRSASIGLFRSFKEDVWARRGLANEKEVTVRALAFITAGHQMHH
                     RIILEERYFPAIPRA"
     gene            complement(4005..4415)
                     /locus_tag="VGR03_09485"
     CDS             complement(4005..4415)
                     /locus_tag="VGR03_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994552.1"
                     /translation="MRGEVIHHASSARPPRSLVVRGGLVRTFGGLASFLVLIFLCGGL
                     YILEDAFANPLDAGTAAVISAAFIITLAAMLLFFLIKPGERPRTEGHDLPFDSPAPAV
                     RHFFDPAAGAMRHDNLRNNLAYQRFYVDHSLIRP"
     gene            complement(4419..4634)
                     /locus_tag="VGR03_09490"
     CDS             complement(4419..4634)
                     /locus_tag="VGR03_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994553.1"
                     /translation="MVMKSGEHWHCVNPAYGCAVLVEANGEVEGQNPRCACGNIMKKS
                     YSPPVLGYLDFLRFPEPALAHRDSPED"
     gene            4887..6104
                     /locus_tag="VGR03_09495"
                     /pseudo
     CDS             4887..6104
                     /locus_tag="VGR03_09495"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            6108..6536
                     /locus_tag="VGR03_09500"
     CDS             6108..6536
                     /locus_tag="VGR03_09500"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994554.1"
                     /translation="MEGIIAVTHIAGKPAHRIVKTRIPNATYCEPQIGSIGLTEKQAR
                     DAGYEVKIGKFPFVGNSKATILGNHGGFIKVVSDATYGEVLGIHIIGPLATELLAEAA
                     TVLNLEGTIDDMMNMMHAHPTVWEAMGDAFASVKGLQINV"
     gene            6557..7279
                     /gene="lipB"
                     /locus_tag="VGR03_09505"
     CDS             6557..7279
                     /gene="lipB"
                     /locus_tag="VGR03_09505"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEV1994555.1"
                     /translation="MKICQIVDLGLIGYAEAYALQKRVVAARKAGAMEDVLLLCEHPH
                     VITLGRSGKRENLLASEHVLRQKGVEFHATDRGGDITYHGPGQIVGYPILNLGAIRRD
                     VVWYVRRLEEAMIRATAEFGIAAERVAGKTGIWVRIGNTEEKLAAIGVHISRWVTSHG
                     FAYNVSTDLRNFDLIVPCGIADRKATSLEKLLGRNVEEKEVAPRIAKHLGELLELEMR
                     EASRKELQEKLEHAEQAVTVSA"
     gene            7294..>8383
                     /locus_tag="VGR03_09510"
     CDS             7294..>8383
                     /locus_tag="VGR03_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV1994556.1"
                     /translation="MKKELTRQQYLDLYYYMRLNRAVEDMMVKLFRQNKIVGGLYSSL
                     GQEAISVGTAYALEKKDWLAPMIRNIGALLVKGVAPRDIFMQHMAKYDSPTKGKDGTS
                     HFGDLDDLHIVSPISMLGDLIPVMTGVAMAGRYLGQKIVAMTWIGDGGSSTGVFHEGL
                     NFAAVQKAPFVLILENNLWAYSTPVRKQVPLENLADRAKAYGIDSYIVDGNDVVAVYS
                     TAKEAVERARAGEGPILIEAKSFRRQGHAQHDAAEYVPKEMRAYWEARDPVTLYEKFL
                     STEKLLDSKSKKEIEEKINTLLAKERDFAENSPMPPPELAETGVYCTGDDCHKSRAKW
                     ERPIAEVTPPRSRVEAVWTVPGFGSGKGS"
BASE COUNT         1800 a         2078 c         2898 g         1607 t
ORIGIN      
        1 gatcgccgtt acggtgacgc tggcgacgtt catggaggtg ctggacacat cgatcgcgaa
       61 cgtggcgctg ccgcacatcg cgggaaatct gtcggcgggc tcggatgaat cgacatgggt
      121 gctgacatcg tacctggttt cgaacgcgat cgtgcttccg ctatcgggat ggttttcggg
      181 agtgatcggg cggaagaagt tttacatggc gtgcgtggcg atatttacag tgagttcgtt
      241 tttgtgcggg ctggcgccaa gcttgggcgt gctggtgatt ttccggattt tgcagggcgt
      301 gggtggcgga ggattgcagc cgagcgagca agcgatcctg aacgatacgt ttccgctgga
      361 gaagcgtggc atggcgtttg cagtgtatgg gctcgccgtg gttgtggcgc caacaattgg
      421 gccgtggctg ggcgggtgga tcacggacaa tttttcgtgg agatggattt tttacatcaa
      481 cgtaccggtg ggaatgcttt cgctcttgct gacaaacttt ttggtaagcg acccgccgta
      541 catgaagaaa tcgagtctga aggcaggatt ccggatcgat tacatcggga tcgggttgat
      601 cagcctgggt ctgggaagca tgcaaatcat tctggacaag gggcagcgcg aggactggtt
      661 ctcgtcgaaa ttcattgtgg ttttgttcct gctgatgctg atcggaattg tcgcgggaat
      721 cctgtgggaa ttgcgcgaga aggagccggt ggtcgacctg aggatgttga aggaccgcaa
      781 ttttgcagtc gccacggtgg cgatgttttt tctgggattc gtgctgtacg cgagcacggt
      841 attgattcca cagtttctgc aacagatgct gggatactcc gcgcaactgg cgggaatggc
      901 gctgtcgccg ggcggagcgg tgatcatgtg catgatgccc gttgtgggaa tcctggtgtc
      961 aaaagtggac acgcggatct taatcacgtt cgggtgcatt gtgtcggcgt ccgcgctgtt
     1021 cgtgatggca gggtgggacc ttgggctgga ttacgggcac gcggtgcgtg cgcgcatgct
     1081 gcaaagcttc gggctggcgt ttctgttcat tccgatcaac gtggcggcgt ttgcgtacgt
     1141 gccgaaagaa aagaccaaca tgggcacggg aattatcaac ctggcgcgga acatcggagc
     1201 gagcgtgggg attgccacgg tgacgacgat gctggaccgg ggggcgcagt ttcatcaagc
     1261 gcggctgacg gagcacgtca acgatttcag cgcggcgtat cacaacatgc tgaacgggac
     1321 gcaggcgaaa ctgatcgcgg cgggctcgac ggcggcgcac gccagcgcgc aggcgcacga
     1381 gatgatttac cacacgattc agcggcaggc ggtgatgctg gcgtttatcg ataatttcaa
     1441 gatgctcggc gtcgtgtttt ttgcggtgat tccgattttg gcgctgatga ggaagccgaa
     1501 aatgcgcgcg ggcgctgtac cggttcactg agcaaaaagt ttctgcgcat tggggtcgcg
     1561 caagagtttt gattcgaatg gggggcaacg atggcaacgc aagcggcagt gggaatacaa
     1621 ctgaacgaag agaagttgat ccgcgccgga cagcgaggcg acgagcaggc ggtggaagcc
     1681 ctatttcgcc gttaccagcg gccgttgttt cagacggcac tgcgggtgct gggaaacgcg
     1741 gaagacgcgg aagatgcgct gcaggacggg ctgctctcgg cgtatcgcaa tctaaagcgc
     1801 tttgaggggc ggtcgcagtt ttcaacgtgg ctgacgcgca tcgtgatcaa cgcggcgctg
     1861 atgcggcggc ggagcgcgaa ggcgcgtccg gcggtttcgc tggacgcgcc gccgcgcgag
     1921 aacgagctgc ccgctgcgga gcgcttcgcg gatgacggac cgaatccgga gcaggttttt
     1981 gcaagcacgg agattcgcga gatgatcagc gaaaacctcg acgagctttc gccgctgctg
     2041 cggacggcgt ttgtcctgcg cgaagtgcag ggctattcga cgggcgaagc ggccaagaag
     2101 ctgggggtca cggaaaacac cctgaaggcg cggttgtggc gcgcgcggca ccaactagcc
     2161 gagcggctag gacggcgtct gcggcggatg aaggacgaca tcgcaggcgg catgggtgat
     2221 gcggagtgca gctactgcta gaagatcgag taaaggaagt ttatgaattg aacgaaaaga
     2281 aggccggcgg aagcgctggc ctttttcttt tgatgtaact cagaaaaaat ccctgacctg
     2341 gcgattagcg agaagcggca gcgtagcgac cgaggacgcg gagttcgctg gtggcgctgc
     2401 ggacttcggt gagggcttgg tcgagccggg caacatcggt tgcttcgaca tcgatgaaaa
     2461 actgatattc ccaggggcgg ccgtggatgg gtcggctctc aattttgagc agattcacgg
     2521 cgtgcttcgc gaaaggctca agcgaggcga ggagcgcgcc tgggcggtgg gcgagccgca
     2581 tggcgaggga aaccttgcgg gcgccgggag aaagccacgc accggcttcg ttttccggga
     2641 gcaggagcac gaagcgcgtg aaattttcgg cgttgtcctg gatgctttcg gaaagaatca
     2701 cgcccttgta gtgctcggcg gcgcggcgcc cggcaatagc ggcggaagat ttgtcccctc
     2761 gggcaagcac ttcacggacg ctgcccgccg tgtcttcggc gggaacgcgt ttcaactccg
     2821 gatgggagga aaagaagcgt tcgcattgcg cgagagccat gggatgcgag gcaacggaac
     2881 gaagaccggc gagcgttgcg cccgggacgc cgatcagatg gtgttcgatg ggaaggatgg
     2941 tttccgcgac gatggcgagg tggctctcga gtagaaggtc gtagacgcgc acgaccgagc
     3001 cagctagcgt gttttcgacg ggcgcgagaa atgcgtcggc ggcgacctca tcgatggcgc
     3061 ggaaggcggc gtcaaaggtg gcgcgaggca cggtggtaat ggcatcgccg agaagctgga
     3121 tggcggcagc ttcgctgaaa gcgccgggtt cgccctgaaa ggccacgcgc atggtcttgc
     3181 gatcggatgt gctcataggc aggcgctatc ttgcaataag cggcgcaaaa ccaaaagcaa
     3241 agattcgctc cgggcaggaa aaagtttgcc gaaacgcatc gagtacagcg atacgacatt
     3301 ctcatacgag gtgaatcggc agccgaaatg gctgttgctc tctcttgaag caaaaattta
     3361 acattttcgg caagcgagcc ctggcgtgcg gtcctccgac ttcgcagtgc gctggagttc
     3421 ctcccagcgg tataattctg tctccagaag agaggttcca ccatgaacag acccgaagcg
     3481 accgaatatg cagattacta cgcgaactac atagcgaaag tcccggggtc cgatgtcttg
     3541 agcgttttgg aatcccagcg attgcagatg ctgcagttgt tcgcggggcg aagcgagcgg
     3601 gacggaagct tccggtatgc gccgggaaag tggacggtga aggaagttct cgggcacatc
     3661 acggataccg agcgcatttt tacttaccgg gcattgcggt tcgcacgcgc cgatcaaacg
     3721 gcccttcctg gattcgagca ggatgactac gtacggagtg gcgcgtttgg cgatcggaca
     3781 ctcgcgggcc tcgccgagga gtttggggcc gtgcgcagcg cgagcatcgg cctgtttcgt
     3841 tcgtttaagg aggatgtctg ggcgcggcgc gggttggcca atgagaaaga agtgaccgtg
     3901 cgcgcgctgg ctttcatcac cgccggccac cagatgcacc accgcatcat cctggaagag
     3961 cgctactttc cggccattcc gcgggcctga atcgacaagc aaagttacgg ccgaatgagg
     4021 gaatgatcga cgtagaagcg ttgatacgcc aaattattcc gtaaattgtc gtggcgcata
     4081 gccccagcag ccgggtcgaa aaagtggcgc accgcgggag ccggagaatc aaaaggaagg
     4141 tcatggccct ccgttcgggg cctttcccct ggtttgatga ggaagaatag caacatggcc
     4201 gctagcgtga taatgaacgc tgcgctgatc accgccgcgg ttcctgcgtc gagcgggttc
     4261 gcaaaggcat cctcgaggat gtagagcccc ccacagagaa agatcaagac gaggaagctg
     4321 gcgaggcccc cgaaagtgcg cacgagcccg ccgcgaacga ccagggacct gggaggacgc
     4381 gcactcgaag cgtgatggat gacctcgccg cgcatgggtc aatcctcggg agagtcccgg
     4441 tgggcgagtg ccggctccgg gaatcgcaag aaatcaagat agccaaggac gggcggagaa
     4501 taacttttct tcatgatgtt gccgcaggcg catcggggat tttgtccctc gacctcgccg
     4561 ttggcttcaa cgagaactgc acaaccatac gctggattga cgcaatgcca gtgctcccct
     4621 gatttcatga ccatggccct tttcctccct tcgcaagaac ggctggttgt ccgcaaaggc
     4681 aatcgcaggt tgggcccgta atgagcgggg agctaagata agtataagta aacagaaaag
     4741 caagaggaat ctttagtctt gatcgtacgt atataactag tactaacgca aggccgagag
     4801 gcggcttccg ggggcgaaca gggtggatgt tcgccgcacc ttcgccccag tgggcgcccc
     4861 tcctacggag aagttgagca acaagcatgc cgcggagcca acaggagcgg aggttgctag
     4921 gggggcgggc ggctgttaaa attcgatgtt tgctgggacg cagccgtctg cgccgtaagc
     4981 gtgggcggct ttttctccgt ttgggatggc gttcgcaaag cgaacggccg gcggcccgtc
     5041 ttatcggcct gactccccct cgagcttttt tcgagtgcga ctgcggtgag gaggtactca
     5101 actcaccggt tctggcccct gccgaaggga gactacgagt ggcaaagtac gatgtagcga
     5161 ttatcggaag tggtccgggc gggtacgtgg cggcgatccg ggcgggcgaa ctaggcctga
     5221 aagcggttgt tgtggagaag gacccgtacc tgggcgggac ctgcctgcac gtgggctgca
     5281 ttcccaccaa ggtgctgcta catcacgcgg agctttacga tcactttaag aatggcgcgg
     5341 aattcgggtt cgaggtcagc gggctgaaaa tcaactgggc caacgtcctc gcgcgaaaag
     5401 acaagatcgt caagaagcat tcgaaaggca tcgagttcct tttcaagaag aacaaggtgg
     5461 agtgggtgca gggctgggga aagtacgaag gccccgggcg cgtgagcgtg gagaaggacg
     5521 ggaagaaaac cgagatcgaa gcggcgagca tcctgatggt gagcgggtcc gaggcaaaat
     5581 cgctccccgg cattgaaccc gaccacaaga cgattctcac gaaccgctcg attctccagc
     5641 ttccggagat cccgaagact ctgattgtgg ttggcgcggg cgccgtgggc gtgtagtttg
     5701 catccatcta caattcgttc gggacgcaag tgacgatcct ggaagcgcta ccgcgtgcgg
     5761 ttccggtcga ggacgaagaa atctccgcgg agctgaccaa ggcctttcag aaaagaggga
     5821 tccaggtttt caccagttgc ggagtggagt cggtgaagaa ggatgccaag ggcgtcacgg
     5881 tggctttcaa agacaaggac ggcaaggcac agacgctgca ggcggagaaa ctactcttgg
     5941 cggtgggccg caagccgatg acggatggtt gcggactgga aaaatcgaag gccaagctgg
     6001 agcgcggatt cgtccacgtg ggcgataaca tggagacggc cgagaagggt ctctatgcga
     6061 tcggagacat cgttgcgggg ctgccgcaac tggcgcacgt ggcgatgatg gaagggatta
     6121 tcgcggtgac gcacatcgcc gggaagccgg ctcatcggat cgtgaagacc aggattccga
     6181 acgcgacgta ctgcgagccg caaatcggct cgattggctt gacggaaaag caggcgcgcg
     6241 acgcggggta cgaagtgaaa atcgggaagt tcccgtttgt ggggaacagc aaggcgacga
     6301 ttctggggaa tcacggcgga ttcatcaaag tggtttccga cgcaacgtac ggcgaggtgc
     6361 tggggattca catcatcggg ccgctggcga cggaactgct ggcggaggcg gcgacggtgt
     6421 tgaacctcga aggcaccatc gacgacatga tgaacatgat gcacgcgcat cccacggtgt
     6481 gggaagcgat gggcgatgcc tttgcaagcg tgaaggggtt gcagattaat gtgtagtgac
     6541 tcgtgattgg tgactgatga agatctgcca gattgtcgac ctcggactaa ttggctacgc
     6601 ggaagcgtac gcgctgcaaa agcgtgtcgt ggccgcgcgg aaggccggcg cgatggagga
     6661 tgtgctcctg ctttgcgagc atccgcatgt gattacgctg gggagaagcg gcaagcggga
     6721 gaatttgctg gcgtcggagc atgtactgcg gcagaagggc gtggagtttc acgccaccga
     6781 tcgcggcgga gacattacgt atcacgggcc gggacagatt gttggatatc ccatcctgaa
     6841 cctgggggcg atccggcggg atgtcgtgtg gtatgtgcgg aggctcgaag aggccatgat
     6901 ccgggcgacc gcggagttcg ggattgcggc ggagcgcgtt gcggggaaaa caggaatttg
     6961 ggtgcggatc gggaataccg aggagaaact ggcggcaatc ggcgtacaca tcagccgctg
     7021 ggttacttcg cacggatttg cgtataacgt gtcgacggat ttgcggaatt tcgatctgat
     7081 cgtgccgtgt gggatcgcgg accgcaaagc cacgtcgctg gagaaattgt tggggcgtaa
     7141 cgtcgaggaa aaggaagtcg cgccgcggat cgcgaagcat cttggagaat tgctcgagct
     7201 ggagatgaga gaagcttcga ggaaagaact gcaggagaag ctggagcacg ccgagcaggc
     7261 cgtcacggtt tccgcatagg aattggagca gtgatgaaga aggaattgac gcggcagcag
     7321 tatctggatt tgtactacta catgcggctg aaccgcgccg tcgaagacat gatggtgaag
     7381 ctgttccggc agaacaaaat tgtcgggggg ctttactcca gtctgggcca ggaagcgatt
     7441 tccgtgggca cggcgtacgc gctggagaaa aaggactggc tggcgccgat gatccgcaac
     7501 atcggagcgc tgctggtgaa gggcgttgcg ccgcgcgaca ttttcatgca gcacatggcc
     7561 aagtacgatt cgccgaccaa gggcaaggac ggcaccagcc acttcgggga tctggacgat
     7621 ctgcacatcg tttcgccgat ttccatgttg ggggatttga ttccggtgat gaccggcgtg
     7681 gcgatggcgg ggcgttatct tggccaaaag atcgtggcca tgacctggat tggggacggg
     7741 ggcagctcga ccggcgtgtt tcacgaaggg ctgaattttg cggccgtgca gaaagcgccg
     7801 tttgttttga ttctcgagaa taatctgtgg gcgtattcga cgccggtccg caagcaggtt
     7861 cctctggaga atttggcgga ccgcgcgaag gcctacggga tcgacagtta catcgtggac
     7921 ggcaacgacg tcgtggcggt ctactccacg gcgaaagaag ccgtggagcg cgcgcgggcg
     7981 ggcgaagggc caatcttgat cgaagccaag agcttccgcc gccagggcca cgcgcagcac
     8041 gacgcggccg agtacgtgcc gaaggagatg cgcgcgtatt gggaagcacg cgaccccgtc
     8101 acgctgtacg aaaagttttt gtcgaccgaa aaacttctcg attccaagag taagaaggag
     8161 atcgaggaga agatcaacac cctgctcgcc aaggaacggg actttgcgga gaactctccg
     8221 atgccgccac cggaattggc ggagacgggc gtctactgca ccggcgacga ttgccataag
     8281 agccgcgcga agtgggagcg gccgattgcg gaagtgacgc cgccgaggtc gcgtgtcgaa
     8341 gcggtttgga cggttccggg attcggctcg ggcaaaggct ccg
//
LOCUS       DASYVO010000289         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_109084, whole genome shotgun sequence.
ACCESSION   DASYVO010000289 DASYVO010000000
VERSION     DASYVO010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109084"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..845)
                     /locus_tag="VGR03_09515"
     CDS             complement(30..845)
                     /locus_tag="VGR03_09515"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEV1994557.1"
                     /translation="MLRALEQIRRMRGGAQSHLMRCDDGYYYVVKFQNNPQHRRILVN
                     ELLGTRVAARLGLPTVPVEIVAVSEELIRLTPELAVELPRARIPCQPGLQFGSRYPGD
                     PRQLTLHDFLPDEQLLEVENLHDFAGMLVFDKWTCNTNGRQTVFFSDQGKTAEQAVTR
                     YKTKMIDQGFCFNAGEWNFPDAPLRGLYARNRVYQGVTGMESFAPWLERLEKQMTERA
                     LDEIIRSIPPEWYADDYDALLRLLEQLHRRRQRVPDLILEAKRCNRQPFPNWT"
     gene            complement(1058..1363)
                     /locus_tag="VGR03_09520"
     CDS             complement(1058..1363)
                     /locus_tag="VGR03_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994558.1"
                     /translation="MDIEKDNFEDDNQLSRLEDEELAGETEEIVETEEEELVIVGEEP
                     EEEAPASKPAPKPAAKKKAKKAAKKKSAPKKKAKKSKPRKKATKKKAKKAAKKKKRR"
     gene            complement(1615..>2596)
                     /locus_tag="VGR03_09525"
     CDS             complement(1615..>2596)
                     /locus_tag="VGR03_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1994559.1"
                     /translation="ASTAISTTVSAIFTPTVSTSPDPLAAAVAAQHLRDLYRLYGDWY
                     LAMAAYNCGPGNVQKGIERTGYADFWELYKRNVLPRETKNYVPIIIALTLIAKDAAHY
                     NIQVEPEAPVPTDVVKPGRAIDLRLVAETIDVDAETLRSLNPSLLRLATPDDPSFELH
                     LPMGTAQKFSAEIADIPPDKWVSWRRHRVETGETLTSIAKKYHVTSAAIADANSLERN
                     AALDAGEKLIIPATQPQSEIKRRLVSYRVRRGDTFLGIADRFNVSADDLRKWNHLKAN
                     KVGRGMVLRIYTLGGAPETSPARARFKSKKKPAQPAAGTTAGGAAASNRN"
BASE COUNT          439 a          789 c          780 g          588 t
ORIGIN      
        1 agcgattcgg cagagttttc attcatgatt cacgtccagt tcggaaacgg ctggcgattg
       61 catctcttgg cctccagaat cagatcgggc acgcgttggc ggcggcggtg cagttgttcg
      121 aggagccgca gcagcgcgtc gtaatcgtcg gcgtaccact ccggcggaat cgaccggata
      181 atctcatcca gggcgcgctc ggtcatctgt ttttcgaggc gctccagcca cggcgcgaag
      241 gactccattc ccgtcacgcc ctggtagacg cggttgcgcg cgtaaagacc ccgcagcggc
      301 gcgtcgggaa aattccattc tccggcatta aagcaaaagc cctggtcgat catcttcgtc
      361 ttgtagcgcg tcaccgcctg ctccgcggtt ttgccctggt cgctgaaaaa aaccgtctgg
      421 cggccgttgg tgttgcaggt ccacttgtcg aagaccagca tcccggcgaa gtcgtgcagg
      481 ttttccacct cgagcagctg ctcgtcggga agaaaatcat gcagcgtgag ctggcgcggg
      541 tctccgggat aacgggagcc gaactgcagc ccgggctggc atggaatccg cgcgcgcggc
      601 aactccacgg ccagttccgg ggtcagccgg atcaactctt cggagacggc gacaatctcc
      661 accggaaccg tgggcaggcc gaggcgcgcc gccaccctag ttccaagcag ttcattgacc
      721 aggattcgac ggtgctgagg attattttgg aacttcacga cgtagtagta gccgtcgtcg
      781 cagcgcatca gatgagactg cgcgccaccc cgcatccggc gtatctgttc caaggcccgt
      841 aacatgcgag gtcatgctaa tggtacttgt tgcttccggc aagcaccacg ctccactcgg
      901 gagctcgatt cctgaatcga gaagcgcggc gttcaagttg ggaagcaaac caggcttttg
      961 gaattccact ctgggaggcg ccattccgta aaaagaaaaa gccggaacga tggctccggc
     1021 cttttcctca cgcggatctc tggtttttga aaaagactta ccgcctcttt ttcttcttcg
     1081 ccgccttttt cgcttttttc ttggtggcct tcttcctcgg cttcgatttt ttggcctttt
     1141 tcttcggcgc ggatttcttc ttcgccgctt tcttggcctt cttcttcgcc gctggcttgg
     1201 gcgcaggctt tgacgcgggc gcttcttcct cgggctcctc gccgacgatc accagctcct
     1261 cttcctcggt ttcgacgatc tcttccgttt cgccggccaa ctcctcgtct tccagccgtg
     1321 acagctggtt gtcgtcttcg aaattgtcct tctcgatgtc cattccatcc tcccctgggg
     1381 gaatctttgg cccccatttg caaaggggtc cacgggtcgc tgttctggcc ggagctgcct
     1441 taaactccac cccgcccttg agccgttgtt accgtactcg ccgggcgttg tcaagcattc
     1501 tgcggaattc cctgcaagag actgccaggg ttgcggggag caggtcgaga gcaaaccgtg
     1561 gcaggagagc caacgacatc cttgccagcc cgtaatctat tcataaaaaa agacttaatt
     1621 gcggtttgaa gcggccgctc cgcctgccgt ggtgcccgcc gcgggctgcg cgggtttctt
     1681 ctttgacttg aaccgcgccc gcgccggcga agtttcggga gccccgccca gggtgtagat
     1741 gcgcagcacc atgccgcggc ctaccttgtt ggctttcaag tgattccatt tgcgcaggtc
     1801 gtccgcactc acgttgaagc ggtcggcgat tcccaggaac gtgtcgccgc ggcgcacgcg
     1861 ataactcacc aggcggcgct tgatttctga ttgcggctgc gtcgcgggaa tgatcagctt
     1921 ctcgcccgca tcgagcgcgg cattgcgctc cagactgttc gcatcggcga tggccgcaga
     1981 cgtcacatga tatttcttgg cgatggacgt gagcgtttct ccggtctcca cgcgatggcg
     2041 ccgccagctc acccacttgt ccggagggat atccgcgatc tccgcggaga atttttgcgc
     2101 cgtgcccatc ggcagatgca gttcgaagga aggatcgtca ggcgtcgcga ggcgcagcag
     2161 agagggattc agcgagcgaa gtgtctccgc gtccacatcg atggtttccg ccaccagccg
     2221 cagatcgatg gcccggcccg gtttcacgac gtcggtcggg accggcgcct ccggttcgac
     2281 ctggatgttg tagtgcgccg catccttggc gatgagcgtc agcgcgatga tgatgggaac
     2341 gtagtttttc gtttcgcgcg ggagcacgtt gcgtttgtag agctcccaga aatccgcgta
     2401 accggtgcgc tcgatgccct tctgcacgtt gccggggccg cagttgtaag cggccatcgc
     2461 caggtaccag tcgccgtaga gcctgtagag gtcgcgaaga tgctgcgcgg cgacagcggc
     2521 ggccaaggga tccgggctgg tcgatacggt gggcgtgaaa atcgcgctga cggtggtgct
     2581 gatggctgtg ctggcc
//
LOCUS       DASYVO010000290        12307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_52095, whole genome shotgun sequence.
ACCESSION   DASYVO010000290 DASYVO010000000
VERSION     DASYVO010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 12307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12307
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52095"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..222
                     /locus_tag="VGR03_09530"
     tRNA            148..222
                     /locus_tag="VGR03_09530"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:180..182,aa:Lys,seq:ttt)
     gene            446..1102
                     /locus_tag="VGR03_09535"
     CDS             446..1102
                     /locus_tag="VGR03_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994560.1"
                     /translation="MTVATSSPLSDVSRRVLQLQVITLIWMSVEAIISLLSAWHSHSP
                     ALFAFGGDSLIELLSAAVVFWRFKFNLGEMRAARIAGALLFALAGLVVLTSILNLFGY
                     LEAQRSLLGIAILLGAAVVMPWLASRKRSLAAITSSAALNADAAESSLCGYLAWIALG
                     GLAVNAIWAKSWADPAAALALVPLILREGWEAVHSSKLGCDCCGALTSATTASSARQP
                     "
     gene            1167..1574
                     /locus_tag="VGR03_09540"
     CDS             1167..1574
                     /locus_tag="VGR03_09540"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
                     /protein_id="HEV1994561.1"
                     /translation="MKSLTIGRLAKQAGVNLETVRFYERRGLLPRPPRSASGYRLFPA
                     DAARRLRFIRRAQDLGFSLTEIGELLSLRVSRRTTSADIRTRAEAKIVDIEAKIKSLE
                     SMKKTLRKLTRVCDGCSPVAECPILESLDREEV"
     gene            2006..2260
                     /locus_tag="VGR03_09545"
     CDS             2006..2260
                     /locus_tag="VGR03_09545"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV1994562.1"
                     /translation="MCTKKKIEVFSAGCSTCKETIELVNRIAGSSHEVVIHDMHKSEV
                     ASQAKRYGVRSVPAIVVDGKLAGCCAGRGPDEHVLRSALV"
     gene            2424..3545
                     /locus_tag="VGR03_09550"
     CDS             2424..3545
                     /locus_tag="VGR03_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263499966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:2880..2882,aa:Sec)
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV1994563.1"
                     /translation="MVTNTAAQAATVVIYDDAVTNLAAASFSASSNDLWVTLTDLTRT
                     TGFVVKPQGVCRNELCFPLPKQKKADFLVRRGPVMWFNLSEFARLIKQPVAHDERLGV
                     WYFGPRPDVQNGYLSSLEAPNFTLPDMNGARHSLADFRGKKVLLLTWASWUGCRFDLP
                     GWQTFYHELKGKNFEIVSVAEDTGGAMDAGPWIIKAEPEYTVLLDQEHLVTKLYGMVN
                     VPTAVWIDEQGRIVRPDEVAFVDDRFKSFSHMDAAPYLDAIRDWVAKGKRSPFVLGEQ
                     ELKERLSPVSSDRGMADAEFSLAEYLYHAGHGTEAIPHFKEAQRLAPDNWNYKRQAWS
                     LANAEMDYGTSFQNEVQKLNGKPYYPPRKMPEPAKIPNQ"
     gene            complement(3606..5324)
                     /locus_tag="VGR03_09555"
     CDS             complement(3606..5324)
                     /locus_tag="VGR03_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994564.1"
                     /translation="MAEQIAAGEQGAASASPLRIGIMVDSLIAPLWLAKIISDIQESS
                     FAKIVLVVLNAAPPETKKKPMRDRLKGYWARGLFEHYERWDRQRNHAALDAFTETDVS
                     AALKHVPTLPVVPIRKRFVDRFAPEDIASIRQANLDVLFRFGFRIIKGEILSTARFGV
                     WSIHHGDNREYRGAAPGFWEMYERNPVTGSILQVLNESLDGGKVLYRSLSATDFGSLH
                     STNNSMYWKTADFAIRRLRDLHRFGWNYIQSLEEFNEPVKYTKQIYRTPATPAMVRFL
                     TRQAGRKLARRARSLLVSERPQWFIASRARTGSRTALTDRSGFRAVVAPHGRFYADPF
                     LIKRNGTNYLFFEDFEVAKDRAVISCMEVGKDGTLGVPRTALETDYHLSYPFVFEHRG
                     EVYLIPETRTRKRIELYRAAEFPLKWVLVKMLAENVQAVDSTLHQSAGKLWLFANIAV
                     PGASTFDELHLFLADSLQGEWTPHPKNPIVSDVRRARPAGALFFENGKLIRPSQDCTV
                     RYGRAMTLNEVQVLSATDYREAPVGRIEPDWHPGIVCTHTYNRNEEFEVLDAMEIKRG
                     LRFFTP"
     gene            complement(5459..6616)
                     /locus_tag="VGR03_09560"
     CDS             complement(5459..6616)
                     /locus_tag="VGR03_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1994565.1"
                     /translation="MFSDRTSWNLAPNRLSEALARHRAAGKPLLDLTASNPTECGFDY
                     NGEAILRALANPAALKYAPNPRGLEIARRSVAAYYAARGDTVHEEDIFLTTSTSEAYS
                     LVFRTLCNPGEELLIPEPSYPLFGFLADLHDVRLARYPLVYDHAWQIDFHALEQAVTP
                     RSRGVIVVHPNNPTGHFARPEEVARLNEICSARRMAIIADEVFLDFALTGKQPASFAA
                     NAGALTFTMSGLSKISGLPQMKFAWLIAGGPEPLKLQALARLEVIADTYLSMSAPVQL
                     AAPAFLDQRHGFQKQLMARVRRNLSELDRQLALQKSCVRRGVEGGWYAVLRVPATRSD
                     EDLAVELLTAKDVYVHPGHFYDFPADGFLIVSLITTEPDFLEGAARVLSIF"
     gene            6912..7817
                     /locus_tag="VGR03_09565"
     CDS             6912..7817
                     /locus_tag="VGR03_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994566.1"
                     /translation="MNQEQFQTRRSCAKVAAGILFAGAFLSASLAAQQEGPMKLPSPP
                     PTAPAPASSRPAAVRPSSSSQISNEPPSIPVEQIIQRFAQHEAEFRTERENYTYTQTF
                     VIQTIDTEGRPDGEYKLKSEIVFTPSGKRYEKVTYAPAPTLERISLSQQDLDDLEHVQ
                     PFVLTTEELPKYDVKYVGRQDLDEVSTYVFDVGPKTLVKNQRYFQGRIWVDDRDFGIV
                     KSYGKAVPDIRKKNNENIFPHFETFRENIEGHFWFPTYTRSDDVLHYTFGDIHIRMTV
                     RYANYQRFNVTIKLGDPTEVKPDHP"
     gene            8068..9540
                     /locus_tag="VGR03_09570"
     CDS             8068..9540
                     /locus_tag="VGR03_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV1994567.1"
                     /translation="MIKPQPHQKGSQMQKVDSAFRDALKLAVENAFAYLEGLEQSPVA
                     ATVDLQTLRSRLAKPLTDRGLPPEQVVTQLIRDVEGGLLGSAGGRFFGWVVGGSLPAA
                     LAADWLASAWDQNAALYATAPAAAVVEEVAGSWLKDILRLPAHASFALVSGCQMAHMT
                     CLAAARHALLAKRGWDVEEKGLYAAPPIQILTSAEQHGSYLRAVRLLGLGRSQVTYLA
                     ADAQGRLLPGALEEALAKDPLVPTIVLLQAGEINIGAYDSFETVIPIAKRHGAWVHVD
                     GAFGLWAAASPRFAHLVKGVALADSWATDGHKWLNVPYDCGYAFVADSESHRASMSYR
                     AAYLTHDADARDEMNWNPEWSRRARGFPTYAALRQLGREGVAALVDRCCDHAHALVMG
                     MGRLPGAQVLWEPVINQGLVRFLDPKPGATEEDHDRRTEEVMAAILASGEAFFGGTTW
                     RGRRAMRVSVSSWQTSEKDVQRVVSAVAGILNAPVPSRSA"
     gene            9542..10249
                     /locus_tag="VGR03_09575"
     CDS             9542..10249
                     /locus_tag="VGR03_09575"
                     /inference="COORDINATES: protein motif:HMM:NF021385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEV1994568.1"
                     /translation="MAALARITGLPWPEIREELWNLGYSRLGKVLSPMECEEIRSLYA
                     LPAMFRSRVDMARFRFGRGEYQYFAYPLPALVRELREGLYERLAATANDWMAALSLPG
                     EFPGSLESFLERCHKSGQKRPTPLILRYRAGDYNCLHQDVYGEVVFPFQVIFCLSKPD
                     EEFTGGELLLVEQRPRAQSMGHAIRLEQGDAVVVTTRYRPAKGSRGHYRTNVRHGVSP
                     IRSGERYTLGIIFHDGA"
     gene            complement(10487..11089)
                     /locus_tag="VGR03_09580"
     CDS             complement(10487..11089)
                     /locus_tag="VGR03_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEV1994569.1"
                     /translation="MGAKLAALPFGYIVLVAYWTVLVAELVGDKAIYTVSSLVLRIPT
                     GTVLGAMTAAFAGKMLAAVLLGKVIARVPSHWTALLSAAAFFSSAVFIWFKEPRPMAP
                     GPPAKDFSLRATAICFATLFFTEWGDPGQIAVAALTVQTQSLWAPWLGGLMALMTKGC
                     LAVTVGLKLRDRLPRKKLRMLASVSCCILGILALTALASR"
     gene            complement(11124..>12307)
                     /locus_tag="VGR03_09585"
     CDS             complement(11124..>12307)
                     /locus_tag="VGR03_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994570.1"
                     /translation="VGILTGTRKDVLVAAGDRGPLPNQRGQGEWGDYLTVRPAFPNQK
                     LFAATGYTLKGPGDGSNRDSTPRFVIFGRAEDAGGAAVPAGGGGAGGGGVVPVGGGGG
                     TPPVGGGGGGAGPVVGVVGPFTDVNQLPVVSASVAAEIKAAVGIPQGPSPHALEAAML
                     PELVTKPGAERWQVKTGQDDDVGTVGMNVINGQDLGEGIVEATLEEMVRIPRPPQMAN
                     VTLPYRNFDRQRQSFTEKTIWRIEVTITDLKREADGDYHLVLKGASGETMIGEVPTAT
                     TTFVGNSPWLENITEARKEVDDKIVSQLSPASFALMDGVWVPRGSMSVQPQDTRRPPD
                     TFCTPPEGSEQQMQPFKTQISPRRVRLTGVGLFDRVHNQTGVSQSNGIELHPILKIEW
                     L"
BASE COUNT         2430 a         3649 c         3786 g         2442 t
ORIGIN      
        1 tggaccggct cgttccgaag cccgccgggg ccgtctctgt ggcgtccact gacaccgcta
       61 ctgacaccag gccatttccg tccccggccc ccgattccaa cgcatgtgca ctaagccgtt
      121 ttcctgtaag atgttctgag cgatttcagg ggcgtagctc agcggtagag cagcggcctt
      181 ttaagccgtt ggtcgtgggt tcgatcccca ccgcccctac caaccgtctc ccatcctgat
      241 agacgattcc gagcttggcg gagcagagtt ggacaagtac gcggcacaga aagtcacgct
      301 gaaaggcacc gtgaagggcg aaacggtgac gattgaaaac gtatctccaa tcaagtagcc
      361 cagtgcgatg gcctagaaag tcgtgcagcc ggctgagaag ctcgttcggc tgcgactgct
      421 gagtgggatg accgaaaggt ttgtcatgac cgtagcgaca tcctcgccgc tttccgatgt
      481 ttcgcggaga gtccttcaac ttcaagtcat cacgctcatc tggatgagtg tggaagcgat
      541 tatttctctc ctgtcggctt ggcattcgca cagtcccgcg ttgttcgctt ttggcggaga
      601 cagtctgatc gagctcctct cggcagccgt tgtgttctgg cggtttaagt ttaacctcgg
      661 tgagatgagg gcagcccgta tcgccggggc gctgcttttc gctttggcgg gacttgttgt
      721 gctcacttct atcttgaatc ttttcgggta tctcgaggcg cagcgcagtc tgctgggaat
      781 cgcaatcctg ctaggcgcag ccgttgtgat gccttggttg gccagccgga aacgatcgtt
      841 ggcggccatt acatcgagcg ctgctttgaa cgccgatgca gccgaatctt ccctctgcgg
      901 ttacctggca tggatcgccc ttgggggatt ggcggtgaac gcgatttggg ccaaatcgtg
      961 ggccgaccct gcagccgctt tggctcttgt cccactgatc ttgcgagagg gatgggaagc
     1021 cgttcattcg tccaaattag gctgcgattg ctgcggagcg ctcaccagcg caacgaccgc
     1081 gtcctccgcg cgccagccct aaaaataatg cttgaccccg taccgtagtc cggggtttag
     1141 acttcactca gaaagtgatt gcggacatga agtcactcac aatcggtcgt ctcgccaagc
     1201 aagccggagt taatctggag accgttcgtt tctacgagcg aaggggcttg ctgccaagac
     1261 cgccgcgcag cgcgtctggt tatcgtctgt ttcctgcgga cgcggcgcgc cgcctcagat
     1321 tcatccggcg cgctcaggat cttgggttct cgcttacaga aataggagag ctgttgtcgc
     1381 tccgtgtgtc acggcgtaca accagcgctg atattcggac acgggctgag gccaagattg
     1441 tagatataga agcgaagatc aagagtcttg aatccatgaa gaagaccttg cggaaactca
     1501 caagagtctg cgatggatgt tcacccgttg ccgaatgtcc cattctcgaa agtcttgata
     1561 gggaggaggt gtgagtacta ggtggaggca aggcttgctc gcactgccga gcgtcggcgt
     1621 gtcgctgctt ccgaaactag cttgtcccgc gtgctggcca gcctatgctg gtctgctgac
     1681 ttccgttggt ctcgggtttc tgatttcggt gatctaccta ttcccgttaa ccactgcgtt
     1741 cctcgtgctc gcattgggcg ccatggtgtt cggagcaaga gagcgccacg ggtacggtcc
     1801 cttgctgctc ggcaccgttg cggcaagcgg tgtcctgcta ggtaagtttg tgtgggagtc
     1861 aaatccaatc atgtacggcg cagtcgggct gctggttatc tcttcgctct ggagtacgtg
     1921 gccgcgtcgc gacgcaccga accaggaggc aagctgttcc gactgcaata cagaagaagt
     1981 tcaaaggagt atctaggaga acaccatgtg cacaaaaaag aaaattgaag tcttcagcgc
     2041 cggttgctca acgtgcaagg aaacaatcga gctcgtcaat cgcatagctg gttcttcgca
     2101 tgaggtggtg attcacgaca tgcataagtc cgaggtagca tcccaagcca agcgttatgg
     2161 cgtccgcagc gtgcccgcca tcgtagttga cggtaaactc gcagggtgct gcgcaggtcg
     2221 cggccccgat gagcatgttt tgcgttctgc gctggtgtag gcatcggctg agttccatct
     2281 ctacgtttgt ctgtggtccg cttgggatct ggcggcaggg caggcgattg gtttgttatc
     2341 cccttcgctg tcgaagcgaa acgggcaagg agagacggat gcgaagactc ttcacgtggg
     2401 tcgggttgat tttcgtcttt ttgatggtca cgaacactgc cgcgcaggcg gccaccgtgg
     2461 tcatttacga cgacgcggtg accaatctgg ccgcagcatc attctcagcc agttcgaatg
     2521 acctctgggt aacactgacc gatctgaccc gtacgactgg ttttgtcgtc aagccgcagg
     2581 gcgtctgccg caacgagctt tgtttccccc tgcctaaaca aaagaaggcc gacttcctcg
     2641 tccgtcgcgg gccggtcatg tggttcaact tgagtgaatt tgcccggctg atcaaacagc
     2701 cagtggcgca cgatgagagg ctcggagtgt ggtacttcgg accgcggccg gatgtgcaaa
     2761 atggctacct atcttcgctc gaggcgccga acttcacgct ccccgacatg aacggcgccc
     2821 ggcactcgct tgcggacttt cgcggaaaga aagtactgct gttgacctgg gcgtcctggt
     2881 gagggtgccg cttcgatttg cctggatggc aaacctttta tcacgagctg aagggcaaga
     2941 attttgaaat cgtctccgtg gccgaggata ccggcggcgc aatggatgcg ggcccctgga
     3001 tcataaaagc agaaccggaa tataccgttc tgctcgacca ggagcatctg gttacgaagc
     3061 tctacggaat ggtgaacgtt cccaccgcag tctggatcga tgagcaggga aggatcgtgc
     3121 gccccgacga agtcgccttc gtcgacgatc ggttcaagtc attttcgcac atggacgcgg
     3181 ccccatatct cgacgccatc cgcgactggg tcgcgaaagg gaagcgcagc cccttcgttc
     3241 tcggggagca ggagctcaag gaacggctgt cgccggtgag ctccgatcgc gggatggccg
     3301 atgccgaatt cagcctcgcg gaatatctct atcatgccgg ccacggcacc gaagcgatcc
     3361 cgcacttcaa ggaagcacag cgcctcgccc ccgacaactg gaactacaag cgccaggcgt
     3421 ggagccttgc gaatgcggag atggactacg ggaccagttt ccaaaacgaa gtgcagaagt
     3481 tgaacggcaa accatactac ccaccgcgga agatgccgga gcctgcgaaa atacccaacc
     3541 aatgaatccc ggggcggata ggagcggcgt cgggcaggtc ttgcgaactc cgaacatcgg
     3601 aggatttacg gtgtgaagaa tcgaagaccg cgcttgatct ccatggcatc cagaacttcg
     3661 aactcctcgt tccgattgta ggtgtgcgtg cacacgattc ctggatgcca atcgggttcg
     3721 atgcgtccga caggcgcctc gcggtaatcg gtcgcggaga gaacctgcac ttcgttgagc
     3781 gtcatggctc ggccgtagcg aacggtgcaa tcctgactcg ggcggatgag tttgccgttc
     3841 tcgaagaaca gcgcgccggc cggccgcgct ctgcgcacgt cggacacaat gggatttttt
     3901 ggatgtggcg tccattcgcc ctggagggag tcggcaagga acaggtgcaa ttcgtcgaag
     3961 gtggaagcgc ccggcacggc aatgttggcg aacagccaga gtttgcccgc gctttgatga
     4021 agcgtcgaat cgacagcctg cacattttcg gccaacattt tcaccaggac ccacttgagc
     4081 ggaaattcag cggcgcgata cagctcgatt ctcttgcgcg tcctggtctc ggggatcaag
     4141 tacacctcgc cccggtgctc gaaaacaaaa ggatatgaaa ggtggtaatc cgtttcgagc
     4201 gcggttctag gcacgccgag tgttccgtct tttccgacct ccatgcagga aatcaccgcg
     4261 cggtccttcg caacttcgaa atcctcgaag aataaataat ttgtgccgtt ccgcttgatc
     4321 agaaacgggt cggcgtaaaa ccgcccgtgt ggcgcgacaa cggcccggaa gccgctgcgg
     4381 tccgtcagcg cggtgcgcga gcccgttcgc gcgcgagagg cgataaacca ctgtggccgc
     4441 tccgagacca gcagcgaacg ggcccggcga gcgagtttgc gccccgcctg ccgcgttaga
     4501 aaacggacca tggcaggcgt tgccggcgtg cggtagatct gtttcgtgta tttcaccggc
     4561 tcgttgaatt cctcgagcga ctggatatag ttccagccaa agcgatgaag gtcccgcagg
     4621 cggcgaatgg cgaagtccgc cgtcttccaa tacatcgaat tgttggtcga atggagggat
     4681 ccgaaatcgg ttgcggagag agagcgatag agcaccttgc cgccgtcgag cgactcgttc
     4741 aacacctgga ggatgctgcc ggtcaccgga ttccgttcgt acatttccca gaatcccggc
     4801 gcggcgccgc ggtattcccg gttgtccccg tggtgaatgg accagacccc gaatcgcgcg
     4861 gtggacagaa tctctccctt gataatccga aagccgaatc gaaacaggac gtcgagattt
     4921 gcctgacgaa tgctggcgat gtcctccggc gcaaatcgat cgacaaaacg ttttcgaatc
     4981 ggcacaacgg ggagggtggg aacgtgtttc agcgcggcgc tcacgtccgt ttcggtgaaa
     5041 gcgtccaggg cggcgtgatt tctctgacgg tcccaccgct cgtagtgctc gaagagaccc
     5101 cgggcccagt agcctttcaa ccgatctcgc ataggcttct tcttggtttc cggcggggcg
     5161 gcattcagga ccaccagcac aatctttgcg aacgaagatt cctggatatc cgaaatgatt
     5221 ttggcgagcc agaggggagc gatcaaagaa tccaccatga tcccgatgcg cagcggggaa
     5281 gcggaagcag cgccctgctc gccggcggct atttgttcgg ccacgcgcga tctcccctgg
     5341 aatccatgac ctcgcatcct cgctgcggca agaacagccc gttccttgcc gtgccccaat
     5401 tattgaacag tccaccgctc cacaccaagg gctgtgcttg atgaaaatcc atgcactttc
     5461 agaaaatcga gagcacgcgc gcggcccctt ccaaaaagtc tggttcggtt gtaatcaggc
     5521 tcacaatcag aaagccgtca gcggggaagt cgtagaaatg cccgggatgg acgtagacgt
     5581 ccttcgccgt cagtagctcg acggcgagat cttcatccga acgcgtcgcc ggtacccgca
     5641 gcacggcata ccatccgccc tccactcccc gacgcacgca ggatttctgg agggcgagct
     5701 ggcgatcgag ctcggagagg ttccttcgca ctcgcgccat caattgcttt tgaaatccgt
     5761 gacgctggtc cagaaacgcg ggggcggcca actgcacggg ggcactcatg gaaagatacg
     5821 tgtcggcgat cacctcgagc cgcgcgagcg cctgcagttt caacggctcg gggccgccgg
     5881 cgatcagcca ggcgaacttc atctgcggca acccggagat tttggagagc ccgctcatgg
     5941 taaacgtcag cgctcccgcg ttcgccgcga agctggcggg ctgttttccg gtcagcgcaa
     6001 aatccaggaa cacctcatcc gcgatgatcg ccattcggcg ggcggagcag atctcattaa
     6061 ggcgcgcgac ttcttcgggc cgcgcgaaat gtcccgtggg attattgggg tgaaccacga
     6121 tgactccccg ggagcggggc gtaacagctt gttcgagcgc atgaaaatca atctgccagg
     6181 cgtggtcgta taccagcgga tagcgcgcga gcctgacgtc gtggagatcg gcgagaaatc
     6241 caaatagagg atagctgggc tcgggaatca gcagctcctc gcccggattg cagagcgtgc
     6301 ggaaaaccag ggaataggcc tcgctggtgc tggtagtgag aaaaatgtcc tcttcgtgca
     6361 ccgtgtcgcc ccgcgccgcg tagtaggctg cgacggaacg gcgcgcgatt tcgaggcctc
     6421 gcggattcgg ggcatatttc agagcggcgg ggttcgcgag ggcgcgcaga atggcctcgc
     6481 cgttgtagtc aaatccgcac tccgtcgggt tcgatgcggt cagatcgagc aatggttttc
     6541 ccgcagcgcg gtgccgggcc agcgcttcgc tcaggcggtt gggagcaaga ttccagctcg
     6601 tgcgatcgga aaacatttca ggtgagcctc ggggcctcgc tgctcgtcag ggaagcgccg
     6661 gcgggagccg gccgaaatct caaaacaggc ggcacaattc ttggggacat ccaaatcgaa
     6721 ggttagcatg aatcacctca ggaaaagttg aggttgggaa cgccgctgaa attgcggata
     6781 atcgggagga tggtccggcg catagactca cgcagccaat ggttcaaggc aactctgggg
     6841 cgagcaccgc gctccaatca tgtagatggc ccgaaagtca gggccgaact gtcggcagga
     6901 gaatcttgtc cgtgaatcaa gagcaattcc aaacgcgtcg atcgtgcgcc aaagttgcgg
     6961 caggaatact cttcgccgga gcatttctct ctgcctcgct cgctgcccag caagagggac
     7021 cgatgaagct gccgtctcca ccgccgaccg cgccggcgcc ggcatcatcg cgcccggccg
     7081 cagttcgccc cagctcatcg tctcaaattt cgaatgagcc gccttcgatc ccggtggagc
     7141 agatcatcca gcggttcgcc caacatgagg ccgagttcag gacggaacgt gagaactaca
     7201 cctacacgca gacttttgta attcagacca ttgataccga ggggcggccg gacggcgaat
     7261 acaagctgaa aagcgaaatt gtttttacgc cctcgggaaa acgctacgag aaggttacct
     7321 atgcgcctgc gccgacgctc gagcgcatct ccctctcgca gcaggatctc gacgacctgg
     7381 aacatgtcca gcctttcgtg ctcaccaccg aggagttgcc caagtacgac gtcaagtacg
     7441 ttggccgcca ggatctggac gaggtcagca cctacgtgtt cgatgtgggc ccgaaaacac
     7501 tggtaaagaa tcagcgttac tttcaaggtc gtatctgggt ggatgaccgg gacttcggga
     7561 tcgtcaagag ctatggaaaa gccgtgcccg acatcaggaa gaaaaacaac gagaacatct
     7621 ttcctcactt cgagactttc cgggaaaaca tcgaaggcca tttctggttc ccgacctaca
     7681 cacgctcgga cgacgtcctg cattacacct tcggcgacat ccatattcgc atgacggttc
     7741 gctacgcgaa ttaccagcgg ttcaacgtga ccatcaagct cggagatccc accgaggtca
     7801 agccagacca tccctaaccg cagcccgagc ggacccgcgg gctgtaaact ggtttgctgc
     7861 cggagaaata tatctctgca agattcggat agttcccgtc ccgccaaact cctacagttg
     7921 accgatggaa gcgatgacgt ccggcaatgt ggcacgcgtt cgccgcgggc cattttggcg
     7981 gcgtgcgcag cctgcactca atccaatttg gaactcgtgg ccgcgctcga cacttgctat
     8041 tttcgcactt cgaaaggcgg ggcaggaatg atcaagccgc agccgcacca aaaggggtcg
     8101 cagatgcaaa aagtggattc ggcatttcga gatgcactca agctggcggt cgagaatgca
     8161 ttcgcctacc tcgaagggct ggaacagagc ccggtcgctg cgacggtgga tctacaaact
     8221 ctgcgtagcc ggctggcaaa gccgctgacg gacagaggcc ttccgccgga acaggtggtc
     8281 actcaactga tccgggacgt ggagggaggg ctgctgggtt ccgcgggcgg gcgatttttt
     8341 ggctgggtcg tcggcggctc cctgcctgct gctctcgctg cggattggct ggcatcggcg
     8401 tgggatcaga acgccgcgct gtatgccacc gcgcccgccg cggcggtggt ggaggaagtt
     8461 gcaggtagct ggctaaaaga cattctcagg ctcccggcgc acgccagctt tgcgctggtc
     8521 agcggctgcc agatggcgca tatgacgtgt ctcgcggcgg cacgccacgc gctgctggcg
     8581 aagcgcggct gggacgtcga agagaaaggc ctctacgcgg cgccgcccat tcagattctg
     8641 accagcgcgg agcagcatgg cagttacctg cgggcggtcc gcctgctggg tctcggtcgc
     8701 tcacaggtga cgtatctcgc cgcggacgct cagggccggc ttctgcccgg cgcgctcgaa
     8761 gaggcgctcg cgaaagatcc gttggtgccc acgattgtcc tgttgcaagc gggcgaaata
     8821 aacatcggcg catacgattc cttcgaaacg gttattccca tcgccaagcg ccacggcgcc
     8881 tgggttcacg tcgacggcgc gttcggtttg tgggcggcgg ccagtccgcg cttcgcacat
     8941 ctggtcaagg gtgtcgcctt ggcggattcc tgggccaccg atggccacaa atggctcaac
     9001 gtgccatacg actgcggcta cgcattcgtt gccgactcgg aatcgcaccg cgcttcgatg
     9061 tcctaccgcg ccgcgtattt gacgcacgat gccgacgcgc gcgacgagat gaactggaat
     9121 ccggagtggt cgcgccgagc gcgcggcttt cccacctacg cggcgcttcg ccagttgggc
     9181 cgcgaaggcg tcgcggctct ggtcgatcgc tgctgtgacc atgcgcacgc tctggttatg
     9241 ggcatgggcc gcctgcccgg cgcgcaagtc ctctgggaac cggtgatcaa tcaagggctg
     9301 gtacgctttc tcgatccgaa gcccggcgcc acggaagaag atcacgaccg gcgcacggaa
     9361 gaagtcatgg cggcgattct cgcgtcgggt gaggcgttct tcggaggcac cacctggcgc
     9421 gggcgccgcg ccatgcgcgt gagcgtctcc agctggcaga cctcggagaa agatgtccag
     9481 cgcgtggtca gcgcggtcgc ggggatcttg aacgcaccag tgccttcgcg gagcgcttga
     9541 tatggcggcc ttagcccgga tcaccggtct tccgtggccc gaaatccggg aagaattgtg
     9601 gaatctcggc tacagccggc tcgggaaggt gctgagtcca atggagtgcg aggaaattcg
     9661 cagcctgtac gcgctgccgg cgatgtttcg cagccgcgtg gacatggcgc gcttccgatt
     9721 cggccggggt gagtatcagt atttcgccta cccgcttccg gcgctggtta gggagcttcg
     9781 cgaaggactt tatgagcgcc tggccgccac ggcaaatgat tggatggcgg cgctttctct
     9841 gcccggcgag tttccgggga gtttggaaag cttcctcgag cgatgccata agagtggcca
     9901 gaagcgtccg acgccgctga tccttcgcta ccgcgcgggg gattacaact gcctgcacca
     9961 ggatgtctat ggcgaggtcg tatttccgtt ccaagtgatt ttctgtctca gcaagcccga
    10021 tgaagaattc accggcgggg aattgctgct ggtggaacag cggccgcgcg cgcagtcgat
    10081 gggccacgcc atccgcctcg agcagggcga tgccgtcgtg gtcaccacgc gctaccggcc
    10141 cgcgaaaggc tcgcgcggcc attaccgcac gaatgttcgc cacggcgtga gccccatccg
    10201 cagcggcgag cgctataccc tgggaatcat ttttcacgac ggggcgtagg aggatgaaat
    10261 gttgaatgtg gaatgctgct gggaagccgt cgaaaaacgc gacgccggac aggatgacac
    10321 ttttctttat ggggtaatga ccaccggagt gtactgccgc ccgtcgtgcg ccggacgacg
    10381 gccactgcgc aagaatgtac gattctatga aaccggcgcc gccgcggaag acgacggact
    10441 gcgtgcctgc aagcactgcc gtcccctggc caggcgtgcg cgattctcag cgggaggcca
    10501 gcgcggtgag cgcaagaatg ccgaggatgc aacacgagac tgacgccagc atgcgcagct
    10561 tttttctcgg gagccggtcg cgcaatttca atccgaccgt cacggccagg caacccttgg
    10621 tcatcagcgc catcaacccg cccagccacg gcgcccacag cgactgggtc tggacggtca
    10681 gcgcggccac agcaatttga ccggggtctc cccactccgt gaaaaagagc gttgcgaagc
    10741 agatcgccgt cgctcgcaag gagaagtctt tcgccggcgg tccgggggcc atggggcgcg
    10801 gttccttgaa ccagatgaac accgcggagg agaagaaggc tgcggcactc agcagcgctg
    10861 tccagtggga cgggacccgc gcgatcacct tgccgagcag aacggcggcg agcatcttcc
    10921 cggcgaaggc tgccgtcatg gcgcccaaca ccgtaccggt gggaatgcgc aggacgagcg
    10981 aactgactgt gtagatggcc ttgtctccca ccaactcggc gaccaggacc gtccagtagg
    11041 ccaccaggac gatgtagcca aaaggcaaag ccgctagttt tgcgcccacg atgcgcgcct
    11101 tttctccccg agatcgccgc gtgtcagagc cactcgatct tcaggatcgg atgaagctcg
    11161 atcccattgg attgcgacac tcccgtctgg ttgtgcactc ggtcgaagag gcccacgccc
    11221 gtgagccgca cgcgccgcgg actaatctgg gtcttgaagg gctgcatctg ttgttccgac
    11281 ccttccggag gggtgcaaaa cgtatcgggc ggtctgcgcg tgtcctgcgg ctgcacggac
    11341 atcgacccgc gcggcaccca cacgccgtcc atgagcgcaa aggaagcggg agaaagctgg
    11401 ctgacaatct tgtcgtccac ttctttgcgg gcctcagtga tgttttcgag ccacgggctg
    11461 ttgccgacaa acgtggtcgt ggcggtggga acctctccga tcatcgtctc cccactggcg
    11521 ccttttagga ccaggtggta gtccccgtct gcctctcgtt tgagatcggt gatcgtaact
    11581 tcgattcgcc agattgtttt ttcggtgaac gactgccgtt ggcgatcgaa gttacggtag
    11641 ggcagggtca cgttcgccat ctgcggcggg cgcgggatgc ggaccatttc ctccaaagtg
    11701 gcctcgacga tcccctcacc gaggtcctgg ccgttgatca cgttcattcc cacggtaccc
    11761 acgtcgtcat cctggcccgt ttttacctgc cagcgttccg ccccgggctt ggtaaccagc
    11821 tcgggcagca tggccgcctc cagcgcgtgt ggcgacgggc cttggggaat tcctaccgct
    11881 gccttgatct ccgcggcgac gctggcgctg accacgggca actggttgac gtcggtgaac
    11941 ggaccgacta ctcccacaac gggcccggcc ccgccgccac ctcctcccac cggcggggtt
    12001 ccgccgcctc ctccgaccgg caccacgccg ccaccgccgg ctcctcctcc gcccgccggc
    12061 accgccgcgc ctcccgcgtc ttcggcgcgg ccaaaaatca cgaatcgcgg cgtggaatcc
    12121 cgattcgaac cgtcgccagg acccttcagc gtgtagccgg tagcagcaaa aagtttttgg
    12181 ttggggaagg ctggccgcac ggtcaggtaa tctccccact ctccctggcc gcgctgatta
    12241 ggaagcgggc cgcggtcgcc ggcggcgacc aggacgtctt tgcgcgtgcc ggtgaggatg
    12301 cccacta
//
LOCUS       DASYVO010000291         9387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_231830, whole genome shotgun sequence.
ACCESSION   DASYVO010000291 DASYVO010000000
VERSION     DASYVO010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9387
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231830"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /gene="hemG"
                     /locus_tag="VGR03_09590"
     CDS             complement(<1..551)
                     /gene="hemG"
                     /locus_tag="VGR03_09590"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEV1994571.1"
                     /translation="MTRHVPALVVGAGISGLVCTYALRKAGIDAQLVEASQRPGGVIN
                     SVTRDGFLLELGPQSFSGTLALRVLCQDLGISDQVVQAPPRAPRYVLIDGQLRPVPLS
                     PPAFFMSSLINASTKWALVRDILGKSTPPQGDESVAALVRRKFSPQLLDRLVGPFVSG
                     IYAGDPERLSVRSAFPQLYEAEKA"
     gene            complement(576..1988)
                     /gene="hemN"
                     /locus_tag="VGR03_09595"
     CDS             complement(576..1988)
                     /gene="hemN"
                     /locus_tag="VGR03_09595"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV1994572.1"
                     /translation="MTLAHPSLQQMRGELHVGEEFLAKYNRPGPRYTSYPTAPVWNDS
                     FGPADLEKVPEEAELARTPVSLYMHIPFCESLCLFCACNVVIQKNKSVAPPYLDVLKQ
                     EMKRISRSVSKNRPVVQFHWGGGTPTYLTPEQIEDLFAFTKEHFHFDLDSEIGIEVDP
                     RVTSREHLETLRKLGFNRLSMGIQDFHAEVQKAVHRIQPYEMTRDLLLAARDLGFDSI
                     NVDLIYGLPYQTADTFAHTVDQILELAPDRIALFSYAHVPWLKKQQGSFAAHLPEGME
                     KFEIFRVGLLKFIEAGYLYIGMDHFAKPGDELAVSQQNRTLHRNFQGYTTKAGADLYG
                     MGITAISGIQDAYAQNYRDIPSWEKAVAERGLATMRGYQLSNEDRLRRAVISRLLCHT
                     IVIKDEISREFGIDFDQHFAEELRRLEPSRQDGLVLLENGEIRATWLGRIFIRNLAMV
                     FDPYLEKQQLIAKPLFSKTL"
     gene            complement(2001..3074)
                     /gene="hemE"
                     /locus_tag="VGR03_09600"
     CDS             complement(2001..3074)
                     /gene="hemE"
                     /locus_tag="VGR03_09600"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEV1994573.1"
                     /translation="MNLQAASINRKEFFLRACRGEVLPRVPVWMMRQAGRYLPEYREI
                     RAKHAFLEVCKTPQLAVEVSIQPFRRLGVDAVIVFSDILIPAEAMGLQLELGDAGPNL
                     PNPVRSQADVDRLKIFDPETETGFLPEAIRRIVKSVGPEVPVLGFAGAPWTLACYMVE
                     GKTKEGFATVKSFLYHEPKTFRELLHRIAQATIPYLKAQIAAGATAVQLFDTWCGELN
                     LQDYEDFALPAVQEIIAGLGGSVPVIYYTKASQHLLPAVARSGANVLSVDWRMDLAEI
                     RRTLGPKIALQGNVDPAVLFAPPEKIREVTLATINSLAGHGHILNLGHGILHHTPVEN
                     AQLFVETGQQALLHHARPAAQVW"
     gene            3268..4080
                     /gene="ccsA"
                     /locus_tag="VGR03_09605"
     CDS             3268..4080
                     /gene="ccsA"
                     /locus_tag="VGR03_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEV1994574.1"
                     /translation="MNHLFPILTAVAYLLSFGLYVRFLGTGSKVTGRLGTLLLGLGLI
                     AHYFALLERSRGLHTVPYHDLYGSMSLFGWLLALTYLGLELYHRQRSVGAFVLPFIVV
                     FFLAAHLAPADRLSPPAMHGVVFALHVTLSILAYAAFALSFVLSLIFLGEERLLRSHK
                     LGDVVWRLPPLELLDQMSRSSVLVGLISIAIGTALGFVSIDQLPGQNSFYDPKYVITL
                     LVLALYALYFRLAGTAAWRGARASKLCIFNFVIVVLSFTVVNLYLSHSHRYF"
     gene            4099..5376
                     /gene="hemA"
                     /locus_tag="VGR03_09610"
     CDS             4099..5376
                     /gene="hemA"
                     /locus_tag="VGR03_09610"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEV1994575.1"
                     /translation="MEIVLVGLNHRTAPVEVRERVSFTAEQARRAAEELRARGILEET
                     LVLSTCNRSEVYGVPPESSHECAPGLSTFLSEFHSVRPDILSVSLYHHYDREAVRHLF
                     RVSAGLDSMLLGEAEILGQVREAYRFAHEHGATGPVLNRLFQGALEVGKRVRTETELG
                     TRPMSVASAGVKLAERIFGKLNERKALVLGAGTISEQVVSHLRDRGIAQLHVMNRSRD
                     RAEELARQFGGKVVGWGEWDAALQSPDVVVSSVSAEEPVLRCEIVERAMAARGNRALF
                     LMDLGVPRNIDATVGDLYNVYVYNTDDLSEIVQQNRNARESEVPKAQGIVDEHVTKFL
                     SWQASVELVGLVDALRTKMREERASFIRSRMEPMKHLTETDRAQVEKLMDEMLEKLLL
                     EPAERLRGEKELRRKIQNVEALRDLFLSNREKP"
     gene            5373..6290
                     /gene="hemC"
                     /locus_tag="VGR03_09615"
     CDS             5373..6290
                     /gene="hemC"
                     /locus_tag="VGR03_09615"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEV1994576.1"
                     /translation="MRILRIGTRGSMLAKWQAEFVRKQLFAATGMEAEIVIIKTSGDK
                     MQQAPLTQIGGKGIFIKELEEALLEETIDLAVHSVKDVPTDIPSRLMFPAVCRRDDVR
                     DCLVGSTLANLRQGARVGTSSLRRQAQLKHLRPDLDLRDLRGNVDTRLRKVESGEYEA
                     VMVAKAGLDRLGLSQRISEVLSPEVCMPAVGQGAIAVECRLKDTEAADLLAPLDDAET
                     RTAIIAERALLAALHGGCQVPLGAWARVERGELVLDACVCSVDGVQYVKQRATAPPDQ
                     AAQLGEHMARVLIEAGAQSILEEVSRQRG"
     gene            6283..7110
                     /locus_tag="VGR03_09620"
     CDS             6283..7110
                     /locus_tag="VGR03_09620"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEV1994577.1"
                     /translation="MAEASPNVLAGKRIVITRSAAQSEALARELSARGAIPVVLPLVS
                     FADPEDFAPLDAAIAEIQRFDWMILTSAQAVRAVVKRGEELKLSLIRSESKLRIAAVG
                     PVTAEAARQAGLPVEYAAETHTGAALAEELGNRLQGAKVFLPRSDRANPDLPPALKRH
                     GAHVTEVIAYRTLRATDVDQRKLGQIAAGAADAVLFFSPSAVQYFAALFGGEQLHALQ
                     DKLAITAVGPVTANALREAGIRRTVLAADTTAVAVVEALEEHFAGTVKQAQAGAKKA"
     gene            7107..8081
                     /gene="hemB"
                     /locus_tag="VGR03_09625"
     CDS             7107..8081
                     /gene="hemB"
                     /locus_tag="VGR03_09625"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEV1994578.1"
                     /translation="MSFPTHRPRRLRRSEALRGLVRETRLTTSGLIYPMFVCPGTSVR
                     QEVSSMPGVFQQSVDQIVEECREVESLGIPGIILFGLPESKDPRGASSFGAQGVVQRA
                     IEAIRKAKLKLLVITDVCLCEYTDHGHCGVIENGEVANDSTLTILAQQALSHARAGAD
                     IVAPSDMMDGRVGAIRETLDEHKFENIAIFSYAAKYCSGFYGPFREAAQSAPQFGDRR
                     NYQMDPANAREALKEVELDLEEGADMVMVKPALAYLDVIRRVREAFDVPVGAYNVSGE
                     YAMVKAAAQKGWLDEKRVVLEILTGIQRAGAEIILTYHAKDVARWLKA"
     gene            8100..>9387
                     /gene="hemL"
                     /locus_tag="VGR03_09630"
     CDS             8100..>9387
                     /gene="hemL"
                     /locus_tag="VGR03_09630"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEV1994579.1"
                     /translation="MSTAQPRLKRERKRSREIFERAEKVLVGGVNSPVRAFRSVGGEP
                     LIIEHGSGQHLYDADGNEVLDYVCSWGAMILGHANPAISEAIADQARRGTSFGVTTEL
                     EVELATLITRAIPFIEKIRFVSSGTEATMSAVRLARGVTKRDFIVKFEGCYHGHADSF
                     LSQAGSGLATLGIAECPGVPAALAALTLNVPYNDLRAFERVFLQHKDKIAAVIVEPIA
                     ANMGVVTPETGFLKGLHEISKKNGALLIVDEVITGFRLRNGSVQELFGIEADLTTLGK
                     IIGGGVPVAAYGGRADLMNNVAPLGPVYQAGTLAGNPLAMRAGIAALKQLTKPGLYEE
                     MAQLAGRLASGLRAELAKAGIPAQVNAIGSLATVFFTPEPVRNYTDAKRSDTKRYARF
                     FREMLDRGIFLAPSQFEAAFVSVAHTSQDIDRTLTAA"
BASE COUNT         1886 a         2495 c         3062 g         1944 t
ORIGIN      
        1 gccttttcgg cttcgtagag ttgcgggaag gcgcttcgaa cgctcagccg ctcaggatct
       61 cccgcgtaaa tcccagaaac gaaggggccc accagacggt cgagcagctg gggcgagaat
      121 tttctgcgga cgagggcagc aacggattcg tcaccttggg gaggagtgct ttttccgagg
      181 atatcgcgca cgagggccca tttcgtcgaa gcattgatta gcgaactcat gaaaaacgcg
      241 ggagggctga ggggcacggg ccggagttgg ccgtcgatca ggacatatcg cggtgcgcgt
      301 ggcggggctt gaacgacttg atcagaaatg ccgaggtctt ggcagagcac gcggagcgca
      361 agcgtaccgc tgaaactttg cggccccagt tccaacagga agccgtcgcg agttacggag
      421 ttgatgaccc caccggggcg ctgagaggcc tcgaccagtt gtgcgtcgat tccagctttg
      481 cgcagggcgt aggtgcagac gaggccggag atgccagcgc cgaccaccag tgctgggacg
      541 tgacgggtca cagggctgtg agtaacctcg cgaactcaga gagtctttga aaagagcggt
      601 ttggcgatga gctgctgctt ttcaaggtac gggtcgaaaa ccatggccag attgcggatg
      661 aagatgcggc cgagccaggt agcgcggatt tcgccgtttt ccagaagcac gagaccatcc
      721 tggcgggatg gctccaggcg gcgaagctct tccgcgaaat gctggtcaaa atcgatgccg
      781 aattcgcgtg aaatctcgtc cttgatgacg atggtgtggc acagcaggcg gctgatgacg
      841 gcgcggcgca ggcgatcttc attcgaaagt tgatagccgc gcatggtagc gaggccccgt
      901 tcggcgacgg ccttctccca ggaaggaata tcccggtaat tctgagcgta ggcgtcttga
      961 atgccgctga tggcggtgat gcccatgccg tagaggtcgg cgcccgcttt ggtggtgtag
     1021 ccctgaaaat tacggtgcag ggtgcggttt tgctgcgaaa cggcgagttc gtcgccgggc
     1081 ttcgcgaagt ggtccatacc gatgtagaga tagccggcct cgataaactt gagcaggcca
     1141 acacggaaaa tctcaaattt ctccatgcct tcagggaggt gcgcggcgaa agagccctgc
     1201 tgcttcttca gccagggcac gtgagcgtag ctgaagagag cgatgcgatc cggggcgagc
     1261 tcaagaatct gatccacggt gtgggcaaaa gtgtccgccg tttggtaggg caggccgtaa
     1321 atcaaatcga cgttgatgct atcgaagccg agatcgcgcg cggcgaggag caggtcgcgc
     1381 gtcatttcat aaggctgaat gcggtgaaca gccttttgca cttcggcatg gaagtcttga
     1441 atgcccatgc tcagacgatt gaagccgagc ttgcgcagag tttcgagatg ctcgcgggag
     1501 gtcacgcggg ggtcgacctc gatgcctatt tcactgtcga ggtcgaaatg gaaatgctct
     1561 ttcgtgaatg cgaagagatc ctcgatctgc tcaggtgtca gatacgttgg tgtgccgccg
     1621 ccccaatgga actggaccac ggggcgattc ttcgagacgc tgcggctgat gcgcttcatc
     1681 tcctgcttga gcacgtcaag ataaggcggc gccacactct tgttcttttg aatgacgacg
     1741 ttgcacgcgc aaaagaggca aaggctttca cagaaaggaa tgtgcatgta gagcgaaacg
     1801 ggtgtcctgg caagttcggc ttcttcgggc accttttcga gatcggcggg gccgaaagag
     1861 tcgttccaga ccggcgcggt gggataactc gtgtaacgcg gccctggacg attgtatttt
     1921 gcgagaaatt cctcgccgac gtggagctcg ccgcgcatct gctgaaggct tggatgtgcg
     1981 agtgtcatgc ttagcgtctc ctaccacacc tgcgccgcag gtcttgcatg gtgaagcagc
     2041 gcttgctggc ctgtctcgac aaatagctgg gcgttctcga ccggcgtatg atgcagaatg
     2101 ccgtggccaa gattgaggat gtgaccgtgg ccagccagag agttaatggt ggccagcgtc
     2161 acttcgcgaa tcttctcagg aggggcaaac aagaccgctg gatcgacatt gccttgcaac
     2221 gcaatctttg ggcccagcgt ccttcgaatt tctgccaagt ccatgcgcca atcgacgctg
     2281 agcacattcg cgccggaccg cgccacggcg ggaagcaaat gttgagacgc ttttgtgtag
     2341 taaatcacgg gaaccgagcc gccgaggccc gcgatgatct cctgaaccgc ggggagcgcg
     2401 aagtcttcgt agtcctgcaa attcagctcg ccgcaccagg tatcaaacaa ttgtacggcg
     2461 gtcgctcccg ctgcgatctg cgccttcaga tagggaatcg ttgcctgagc gatgcgatgg
     2521 agtagctcgc ggaaggtttt cggctcgtgg tagaggaaac tcttgaccgt ggcgaatcct
     2581 tccttggtct taccttccac catgtaacag gcaagagtcc acggagcacc ggcaaaaccc
     2641 aaaacgggaa cttcggggcc gacggatttc acgatccgcc ggatggcttc gggcaggaag
     2701 cccgtttctg tctcagggtc gaatatcttg agccggtcga catccgcttg ggagcgcacc
     2761 gggttcggca gatttgggcc ggcgtcgcct agttccaact gcaagcccat cgcttcggct
     2821 ggaatcaaaa tgtccgagaa aacgatgacg gcatcaacgc cgagcctgcg aaagggctgg
     2881 atcgagactt ccaccgcaag ttgcggtgtc ttgcaaacct caaggaatgc gtgctttgcg
     2941 cggatttcgc ggtattccgg aagataacgg cctgcctggc gcatcatcca aacgggaact
     3001 cgcggaagga cctccccgcg gcaggcgcga aggaaaaact cttttcggtt gatcgatgca
     3061 gcttgcaggt tcatattccg cctgatggat caaggatagc aaatcccagc cggtcttgcc
     3121 gagacgagta ccaggggtat cgagctgtgc gagcacccgc aagtgtgggc tatactcgaa
     3181 atgtttaaga atcaagtggg ttctgcggag aagggccgat ggtagcactg tgcgagccag
     3241 gctgagccgg gggctcaggt acacggcatg aatcatttat tccctatttt gacggcggtt
     3301 gcctatctgt tgagtttcgg cctgtacgtt cgcttcctgg gtacgggaag taaggtcacg
     3361 ggcagattgg gtacgctact cctggggctg ggcctgattg cacactattt tgcgcttttg
     3421 gagagatccc gcgggttgca cacggtccct taccatgact tgtacggttc catgtcgctg
     3481 ttcggctggc ttcttgcgct gacctatctg ggcctggagc tctatcaccg gcagcgatcc
     3541 gtcggagcgt tcgttctgcc ctttatcgtg gtgttctttc tcgctgcgca tcttgcccca
     3601 gctgaccggc tttcgcctcc cgcaatgcat ggagtcgttt ttgcgcttca cgtgacgctg
     3661 agcatccttg cttacgcggc gttcgcactt tcattcgttc tcagcctcat ttttcttggg
     3721 gaggagcggc tgctgcgaag tcacaagctc ggggatgtgg tctggcggct tccgccactg
     3781 gaactactcg atcagatgag ccgatcgagc gtgctcgtgg gtcttatttc gatcgccatt
     3841 ggaacggctt tgggtttcgt gtccatcgat caactgccgg gccagaattc gttttacgac
     3901 cccaaatacg tgatcacgct gctggtgctc gcgctctatg cactttactt ccgtctggcg
     3961 ggcacagcgg cgtggcgcgg ggcgcgggct tcaaaactgt gcatcttcaa ttttgtgatt
     4021 gtggtgttga gttttacagt cgtaaatcta tatctctcgc atagccaccg ctacttttag
     4081 gagcgagcga acctcggcat ggaaattgtt ctcgtcggac taaatcatcg caccgcgccc
     4141 gttgaggtgc gtgagcgggt gtctttcacc gccgagcaag cccggcgcgc ggctgaagag
     4201 ctacgagcgc ggggaattct ggaagagacg ctggtgctct ccacatgcaa tcgcagcgaa
     4261 gtgtatggcg tgccgccgga atcttcccat gaatgcgccc cgggactttc cacatttctg
     4321 agcgagtttc attcggtgcg gccggacatc ctgagcgtat cgctctatca ccactacgat
     4381 cgcgaggcgg tgcggcatct cttccgagtt tcggcagggc tcgattccat gctgctgggt
     4441 gaagcggaga tcctgggcca ggtgcgggaa gcgtatcgct tcgcgcacga gcatggcgcg
     4501 accgggccgg tattgaatcg ccttttccag ggcgccctgg aagtcggaaa gcgcgtgcgc
     4561 acggaaacgg aactcggcac gcggccgatg tccgtggcct ctgcaggcgt gaagttggcg
     4621 gaacgcattt ttggaaaact caacgaacgc aaggcgctgg tattgggggc gggcacgatc
     4681 agcgagcaag tggtctctca tctccgggat cgtgggatcg cgcagctcca cgtgatgaac
     4741 cgctcgcgcg accgggcaga ggagctggcc aggcagttcg gcggaaaggt cgtcggctgg
     4801 ggtgagtggg atgccgcgct gcaatcacca gatgtggtgg tgtcgtcggt cagcgcggaa
     4861 gaaccggttc tgcggtgcga gatcgtcgag cgagcgatgg cggcgcgcgg aaatcgcgcg
     4921 ttgttcctga tggacctggg ggtgccacgg aatatagatg ccaccgttgg ggatctttac
     4981 aacgtttacg tctacaacac tgatgacctg agcgagatcg tgcagcagaa tcgcaacgcg
     5041 cgcgaaagcg aggttcccaa ggctcagggc attgtcgacg agcatgtgac caaattcctc
     5101 tcctggcagg cgagcgtcga gctggtcggg ctagttgatg cgctgcgcac gaagatgcgg
     5161 gaggaacgag cgtcgttcat ccgctcacgc atggagccga tgaagcatct gacggagacg
     5221 gatcgcgctc aagtggaaaa attgatggat gagatgctgg agaagctgct tctggagccg
     5281 gcggaacgct tgcgaggcga aaaagaactc cggcgaaaga ttcaaaacgt cgaggcgttg
     5341 cgcgatctgt tcctttcgaa ccgggagaag ccgtgagaat cctccggatc ggcacgcgcg
     5401 ggagcatgct tgccaagtgg caggcggaat tcgtacgcaa gcaactgttt gctgccaccg
     5461 gcatggaagc ggaaatcgtc atcatcaaga ccagtggcga caagatgcag caagcgcctc
     5521 tgacgcagat tggcggcaag ggaattttta tcaaggagct ggaagaggcg ctgctggagg
     5581 aaacgatcga tcttgcggtg cacagtgtga aggacgtgcc gaccgacata ccgtccaggc
     5641 tcatgtttcc tgcggtgtgc cggcgcgatg acgtgcgtga ttgcctggta ggctccacgc
     5701 tggcgaatct gcggcaggga gcgcgtgtgg gaacgagcag tctgcgccgg caagcgcagt
     5761 tgaaacacct ccggccggac ctcgatctgc gcgacctgcg aggaaacgta gacactcgat
     5821 tgagaaaagt ggagagcggg gaatatgagg cggtgatggt tgccaaagcg ggcctcgatc
     5881 gcctcggttt gagccagcgg atttcggagg tgctctcgcc ggaagtttgc atgccggcgg
     5941 ttgggcaggg cgccatcgca gtggagtgcc ggttgaagga cacggaagcg gcagacctct
     6001 tggctccgct ggatgatgca gagactcgca cggcgatcat tgcggagcgc gcactcctgg
     6061 cagcattgca cggtggatgc caggttcctc taggcgcgtg ggcgcgcgtc gagcgcggcg
     6121 agcttgttct tgacgcgtgc gtttgttccg tggacggagt gcaatacgtg aagcagcgcg
     6181 ccacggctcc gccggatcag gcggcgcagc ttggagagca catggcgcgg gttctcattg
     6241 aggcgggcgc gcagtcgatt ctggaagaag tgagccggca gcgtggctga agcatctcca
     6301 aatgtcttgg ccggcaagcg catcgtcatt acgcggtccg cggcgcagag cgaggcgctg
     6361 gcgcgagaac tctccgcacg cggagcgata cccgtggtgc tacctctggt gtccttcgcg
     6421 gaccccgaag actttgcgcc gctcgatgcg gcgatcgcgg aaatccagcg atttgattgg
     6481 atgattctca ccagcgcgca ggcggttcgc gcggtggtaa agcgcggcga ggaactgaag
     6541 ctcagcctga ttcgctcgga gagcaaactt cgaatcgctg ctgtcggtcc agtcaccgcc
     6601 gaagccgctc ggcaggcggg acttcccgtt gagtatgctg cggagacgca taccggtgcg
     6661 gcgctagcgg aggagttggg aaaccggctt cagggagcga aagtattctt gccgcggagc
     6721 gatcgtgcaa atccggattt gccgcctgca ttgaagcgcc acggcgcgca tgtcaccgaa
     6781 gtgatcgcct accggacctt gagggcaacc gatgtagatc aaagaaagtt agggcagatc
     6841 gccgcaggcg cggcggatgc ggttctgttt ttcagcccgt cggccgtgca atatttcgcg
     6901 gcgctgtttg ggggcgagca attgcacgct ctacaagata aactggcgat tacggcggtc
     6961 ggacccgtga ccgcgaacgc gttgcgcgag gcgggtatcc ggcgcaccgt gttggcggca
     7021 gacactacgg cagtggcggt cgtcgaagcg ctagaagaac attttgcggg taccgtgaaa
     7081 caggcgcagg caggagcaaa gaaagcatga gttttcccac ccatcggccc cggcgtttgc
     7141 ggcggagcga agcgctgcgt ggacttgttc gagagacgcg ccttaccacg tcgggtctca
     7201 tttatccgat gttcgtgtgt ccgggtacaa gtgtccgcca ggaagtcagc tccatgcccg
     7261 gtgtctttca gcagtctgtc gatcagattg tggaagagtg ccgcgaggtg gagtcgcttg
     7321 ggattccggg gatcattctt ttcggattgc ccgagtccaa ggacccacgc ggcgccagtt
     7381 cctttggtgc gcagggtgta gtccagcgcg ctattgaagc tattcgcaaa gcgaagctga
     7441 agcttcttgt cattacggac gtgtgcctct gcgaatacac ggaccatggc cactgcggag
     7501 tcatcgagaa tggcgaagtg gccaacgatt ccacactgac gattcttgcc cagcaggctc
     7561 tctcgcacgc gcgcgctggc gccgacatcg ttgctccgtc ggacatgatg gacgggcggg
     7621 tgggggccat tcgtgaaaca ctggatgaac acaaattcga gaacattgcg attttttctt
     7681 atgccgcgaa atattgttct ggcttctacg gacccttccg ggaagcggcg cagtctgctc
     7741 cccagtttgg cgaccggcgt aattatcaga tggaccccgc caatgcgcgg gaagcgttga
     7801 aagaagtgga gcttgatctc gaagaaggcg cggatatggt gatggttaag cccgcgctgg
     7861 cgtacctgga tgtcattcgg cgggtgcgcg aggcgtttga tgttcccgtc ggtgcttaca
     7921 atgtcagcgg agagtacgcc atggtcaaag ctgcggcgca gaagggttgg ctggatgaaa
     7981 agcgggtcgt gcttgagatt ctgacgggca ttcagcgcgc cggagcggaa atcattctta
     8041 cctaccatgc caaggatgtc gcacgctggc tgaaggcgtg ataatttaaa cgagaaaaca
     8101 tgagtacagc gcaaccccga ttgaaacgcg agcgcaagcg ttcgcgcgag attttcgagc
     8161 gcgccgagaa ggtgctggtt ggcggcgtga acagtcctgt gcgggcgttc cgttccgtgg
     8221 gcggagagcc gctgattatc gagcacggca gcgggcaaca tctctacgat gcggacggaa
     8281 acgaagtgct cgactatgtg tgctcctggg gcgcgatgat cctgggacac gcgaatcccg
     8341 ccatcagcga ggcgattgcc gaccaggcgc gtcgcggcac aagttttggc gtgacgacgg
     8401 agctggaggt ggaactggcc acgctgatta cgcgggcgat tccatttata gaaaagattc
     8461 gcttcgtctc gagcggcacg gaagcaacga tgagtgccgt gcggctggcg cgaggagtga
     8521 cgaagcgaga cttcatcgtc aaatttgagg gctgttatca cggacacgcg gacagcttcc
     8581 tgtcgcaggc gggttccggc ctcgctacgc ttggaattgc cgagtgcccg ggggtgcctg
     8641 cggcgctagc ggcgttgacg ttgaacgtgc cctacaacga tctccgcgcg ttcgaacgcg
     8701 tatttctcca gcataaggat aagattgcgg cggttatcgt cgagcccatc gccgcaaaca
     8761 tgggagttgt tactccggag actggtttct tgaagggatt gcatgaaatc agcaagaaga
     8821 acggcgcgct gctcatcgtc gacgaagtga tcacaggatt tcgtttgcgc aatggctccg
     8881 tgcaggaact cttcggcatc gaagctgacc ttacgacact cgggaaaatc atcggtggtg
     8941 gagttcccgt ggcagcttat ggcgggcgcg ccgatttgat gaacaatgtg gcgccgctcg
     9001 gcccggtgta tcaagcgggc acgctcgcag gtaacccgct ggcgatgcgt gctggcattg
     9061 cggctctcaa acagctcaca aagcctgggt tgtacgaaga gatggcgcaa ctggcgggga
     9121 ggctggcctc tggtctccgg gctgaactag caaaagctgg aatccctgcg caggtcaatg
     9181 ccatcggctc gctcgcgacg gtattcttca cgccagagcc ggtgagaaat tacaccgatg
     9241 ccaagcgctc cgacaccaag cgatacgcca ggtttttccg cgagatgctc gatcggggga
     9301 tatttttggc gccgtcacag tttgaggccg cctttgtctc cgtggcgcac acgtcccagg
     9361 atattgaccg cacgctgacg gcggcgc
//
LOCUS       DASYVO010000292         3869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_92713, whole genome shotgun sequence.
ACCESSION   DASYVO010000292 DASYVO010000000
VERSION     DASYVO010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92713"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1378
                     /locus_tag="VGR03_09635"
     CDS             248..1378
                     /locus_tag="VGR03_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEV1994580.1"
                     /translation="MERNIRDLLNLYNPNDPLDHAWTIPSPWYFDPRVGELEQHSVFA
                     RTWQVVGRAYQVRDKGSFFTADLGGEPLVVTRGDDGQLRAFYNVCRHHAAAVVTEAEG
                     CAKQFRCPYHGWTYGNDGALKGMVEFEGVCNFDRAKNGLVPVKVDTWENFVFVNLDGR
                     AVELRDFLGAIPEIVAPLQLTKKLKYFDRRIYTLRCNWKVYVDNYLDGGYHVPHAHKG
                     LSSVIEYTKYTIENFERACLQSSPLSSGSKSESGVAATRQGRAFYLWIYPNFMINAYE
                     GVMDTNLVVPLGVDKCAVVFDYYFGDISKKASKKNQASIKVSEKVQDEDEAICDAVQR
                     GLGSRAYVAGRLSVRREAGEHLFHRLLHADLTSALNPSAAAD"
     gene            1567..2007
                     /locus_tag="VGR03_09640"
     CDS             1567..2007
                     /locus_tag="VGR03_09640"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEV1994581.1"
                     /translation="MAFCKACGTDVGGAAFCPKCGANQGAGIVVAAPSSDGLAENVAG
                     LLCYAVGWVTGIIFLLIDKRPFVKFHAAQSIVVFGALTIFRIGLGIMLGTGGLIGLGL
                     RAAISMLFGLVSFVLWILLMIKAYQHELFKVPVAAGIAEGIAGK"
     gene            2240..3532
                     /gene="tig"
                     /locus_tag="VGR03_09645"
     CDS             2240..3532
                     /gene="tig"
                     /locus_tag="VGR03_09645"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV1994582.1"
                     /translation="MAEATCRRELELEIPAEEVTKKIESVAREFARLANVPGFRRGKA
                     PVSLIRRKFAEDIKGEVLQSLVPERVEKAVAEQKLTPVSQPQVDKLDFNEGQPLKFRA
                     VFEVLPEFELGDYKNLAFEMPTMDVTDEDVTKTLEEMRERAATFAPVEGRAIADGDFA
                     QVKLLGTPDGGGDPLQADSVLCHIGAEETMEPFNANLKGASAGDHKNFDVDYPADYPD
                     PKLAGKKYHYAVEVLGIKTKKLPELNDDFAKDVSDAATLEELKKKVSEGLEHQREHKH
                     RELLHEKVLAEIVKLHDFPVPEALVEHQMDVRLERVVRSLAAQGVDPRAVNLDWVTLR
                     RRQEDHAKDDVKAELIIDRIATAESIDVTDEEVTHELEHMASHGGESAEAIRARLTKQ
                     GTLDRMKAKLRSDKTLDWLAQNAKIKTVAAANAATDSK"
     gene            3616..>3869
                     /locus_tag="VGR03_09650"
     CDS             3616..>3869
                     /locus_tag="VGR03_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEV1994583.1"
                     /translation="MVVEQTSRGERAYDIYSRLLKEHIIFIGTPIDDHVANLATAQML
                     FLQAEDPEKDIQLYINSPGGSITAGFAIYDTMQFVKPDVVT"
BASE COUNT          867 a         1052 c         1171 g          779 t
ORIGIN      
        1 ggggttcaaa tccctccttc cgcaccaaaa tttccgacca ggagattttg ccctgagcga
       61 gcaaagcgag tcgaagggct cttctccgac ccccgcaatt ctagtttgat gacttcacgg
      121 tcgatccgct ctcacccagc ctccaagccc gtccttattc tcttttctcc catcgcgttt
      181 cgcggtatga agtgtgcatg gctttcaaag ggattgtgct cagcgagaga tgggacggga
      241 gaaattgatg gagcgcaata ttcgagacct acttaatctc tacaatccga atgatccact
      301 ggatcatgca tggacgattc cgtcgccctg gtacttcgac cctcgagtcg gggagcttga
      361 gcagcacagc gtgttcgcga gaacgtggca ggttgtgggg cgcgcctatc aggtgcggga
      421 caagggaagt tttttcaccg cggacctcgg gggagagccc ctagtggtga cacggggcga
      481 tgatgggcag ttgcgcgcct tctataatgt ttgccggcat catgccgccg cagtggtcac
      541 cgaggcggaa ggctgcgcga aacagttccg gtgtccctat cacggctgga cttacggcaa
      601 cgatggggcg ctgaaaggga tggtcgagtt tgaaggggtg tgcaatttcg atcgcgcgaa
      661 gaatggactg gtcccggtca aggtggatac ctgggaaaat tttgtattcg tgaatctgga
      721 tggccgcgcg gtggaactgc gggattttct tggcgcgatt ccggaaattg tggcgccgct
      781 gcagcttacg aagaagttga aatatttcga ccgccgcatt tatacgctgc gctgcaactg
      841 gaaagtgtac gtcgacaact atctcgatgg cggctaccac gttccccacg cgcacaaagg
      901 attgagcagc gtcatcgaat acacgaaata cacgattgaa aacttcgagc gcgcgtgttt
      961 gcaatctagc ccgctctcct cgggctccaa atcggaatcg ggagtcgcgg ccacccggca
     1021 aggacgcgcg ttttatctgt ggatctatcc gaatttcatg atcaacgcgt acgaaggagt
     1081 gatggatacg aatctggtgg tgcccctggg ggtcgataag tgcgccgttg ttttcgatta
     1141 ctacttcggg gacatcagca agaaagcctc gaagaaaaat caggcgagta tcaaggtgag
     1201 cgaaaaagtg caggacgagg atgaagccat ttgcgacgct gtgcaacgcg ggctcggctc
     1261 acgcgcgtat gtggcgggcc gcctctccgt acgccgcgaa gccggcgagc atctctttca
     1321 ccgtttgctg catgccgact tgactagcgc gctcaatccc tccgccgccg cagactaaac
     1381 aacccactgc cggcgtggag actccgatgg ccacgactcg aatttgccgt ggtgcgtccg
     1441 gctggacaac gatgcgagat ttggtgaacc gttccttcag cgagattgat aagagcatcg
     1501 agcattgccc cttagcaacc ttttctggta ggcttcgcgc aatccaattt tgcgggaggc
     1561 ccttcgatgg cgttttgcaa ggcgtgtggc actgatgtcg gcggcgcggc gttctgcccg
     1621 aaatgcggtg cgaaccaggg cgcagggata gtcgtagcag cgccttccag cgatggactc
     1681 gcggaaaacg tggcgggatt gctctgctac gcggttggct gggtcaccgg gatcatcttc
     1741 ctcctgatcg acaagcggcc ctttgtgaaa ttccacgccg cgcaatcgat tgtagtcttc
     1801 ggggcgttga ccatttttcg aatcggcttg ggaattatgc tcggcactgg tggcctgatt
     1861 ggtctcggct tacgggctgc tatttccatg ctgtttggct tggtgagctt cgtgctctgg
     1921 attcttctca tgattaaagc gtatcagcac gaactcttta aagtgcctgt tgccgcggga
     1981 atcgcggaag gaattgcggg gaagtagcgc tcgcgtccct ttgcagggct gaatctgatc
     2041 gccgcttctc catcgaattg acgctagggg gagcgtcccc tatactgaga attcgagttg
     2101 cagggtgagt tctcacctgt ttggcctctt ctgaaacgaa agccgggttt gttcgtagcc
     2161 ccagaccggg tcgcgtgccg ccgagaccag caaccgttat gtcaagaatc tgagtggggt
     2221 cccaggaaag gactgttgca tggctgaagc aacctgccgc cgcgagctgg aattggaaat
     2281 tcccgcggaa gaagtaacga aaaaaataga gagcgtggca agggaatttg cgcgcctggc
     2341 gaacgttccg ggattccgcc gcggcaaggc gcccgtctcg ctgattcgcc gcaagtttgc
     2401 agaggacatc aagggagaag tgctgcaatc gctagtgccg gagcgcgtcg aaaaagccgt
     2461 ggccgaacag aagctcacac cggtctcgca gccgcaagtg gataagctgg atttcaacga
     2521 agggcagccg ctgaagttcc gcgcggtctt cgaagtgctg ccggaattcg aactgggcga
     2581 ttacaaaaac ctggcgttcg agatgcccac gatggacgtt acggacgagg acgtgacgaa
     2641 aacgctggaa gagatgcgcg agcgtgccgc gacatttgcg cccgtcgagg gccgcgccat
     2701 cgcggacggc gactttgccc aggtgaaact gctcggcact cctgacggcg gcggcgaccc
     2761 gctgcaagcc gacagcgttc tctgccacat tggcgccgaa gagaccatgg agccgttcaa
     2821 cgccaatttg aaaggcgcga gtgccggcga tcacaagaat tttgacgtcg actaccccgc
     2881 cgactacccg gatcccaagc tggcgggaaa gaagtatcac tacgccgtcg aagtgctggg
     2941 catcaagacg aaaaagttgc ccgaactgaa cgacgatttc gcgaaagatg tcagcgacgc
     3001 ggccacgctg gaggaactca agaaaaaggt cagcgagggc ctggagcatc agcgggaaca
     3061 caagcacaga gaactactcc acgaaaaggt tctcgcggaa atcgtgaagc tccacgactt
     3121 ccccgttccc gaagcgctcg tcgaacatca aatggacgtg cgactggagc gcgtggtgcg
     3181 ctctctggct gcgcaaggcg tcgatccgcg cgccgtgaat ctggactggg tgacgctgcg
     3241 tcgtcggcag gaagaccacg ccaaggatga cgtgaaggcg gaactgatta tcgaccgcat
     3301 cgcaacggcg gaaagcatcg acgtgacgga cgaagaggtc acgcatgaac tcgagcacat
     3361 ggccagccat ggtggcgaat cggcagaagc gattcgcgcg cgtttgacaa agcagggcac
     3421 gctcgatagg atgaaagcca agctgcgcag tgataagacg ctcgattggc tcgcgcagaa
     3481 cgccaaaatc aagactgtcg cggcagcgaa tgcggcgacg gattccaagt gacacactcc
     3541 ggccgagtgc cagcggcttt tacccaaatg aaaaatttcg aagaacccga gtatcgcgga
     3601 acaacgctta tacccatggt ggtcgaacag accagccgcg gcgaacgcgc ctacgatatc
     3661 tattcacggc tcctcaagga gcacatcatt ttcatcggga cgcccattga cgaccacgtc
     3721 gccaatctgg ccacggcgca gatgctcttc ctgcaggccg aagacccgga gaaagacatt
     3781 cagctttaca tcaactcgcc gggcggctcg atcaccgcgg gattcgccat ttacgacacc
     3841 atgcagttcg tgaagccgga cgtggtgac
//
LOCUS       DASYVO010000293         7628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_165153, whole genome shotgun sequence.
ACCESSION   DASYVO010000293 DASYVO010000000
VERSION     DASYVO010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7628
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165153"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VGR03_09655"
     CDS             complement(<1..236)
                     /locus_tag="VGR03_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994584.1"
                     /translation="MTTATLPTLTKKDFQTDQEVRWCPGCGDYAILAAVQSVFPELGV
                     PREKFVVVSGIGCSSRFPYYMNTFGFHSIHGRAPA"
     gene            complement(237..2099)
                     /locus_tag="VGR03_09660"
     CDS             complement(237..2099)
                     /locus_tag="VGR03_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEV1994585.1"
                     /translation="MSTVTELPLAKHKPEVIEQAVIRFAGDSGDGMQITGSQFTNTVA
                     LYGNDIATFPDFPAEIRAPAGTLPGVSGYQLHFSSSDVYTPGDAVDVLIAMNPAALKM
                     NLGDLKANGILIVNRDSFKENDLRKAQMTANPLEDHSLDKFRLFPVELQRLTRAALQH
                     LGIDAKTTDRCKNFFALGMCYWLYNRSMDPTVRWIEEKFKSKPLLAEANKLALRAGYS
                     YCEATEAFQVSYEIPPAQLDPGVYRSLSGNQALALGFVTAAQKAGLTLFQGSYPITPA
                     SDILHELSQYKDFGVMTFQAEDEIAAITSSIGAAYAGALAITTTSGPGMALKTEAMGL
                     AVMLEIPLVIVNVQRGGPSTGLPTKTEQADLLQALFGRNSEAPIPVLAASTPGDCFWV
                     AIEASRIAIKYMVPVIVLSDGYLANGAEPWHIPQLSDIPEIPVSFEKNPEGFLPYKRN
                     PQTLARPWAIPGTPGLEHRIGGLEKQDVTGNVNYEPMNHENMVRIRAAKVAAVAQDIP
                     DAVPVGDAEGDLLIVGWGSTYGSITVALKAQRAKGRRIGHLHLRHLNPLPSNLGEVLK
                     RYKKVLVPELNMGQLLWVLRAKYLVDAVGLNKIQGRPFKQNELEVKIEELLGVS"
     gene            complement(2361..3344)
                     /locus_tag="VGR03_09665"
     CDS             complement(2361..3344)
                     /locus_tag="VGR03_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994586.1"
                     /translation="MKRSASVTAAAVVALICGSLALLFAGLGAIGVLLLRPEDFSSAQ
                     PMPALAKTAGMIGIGILFGWNAWVIVTGIGLLRLRNWARISILIISGITAFFAVTGLL
                     VALNLTFPPPPGAGPDFRMFMHVFLSIFYGAPALMAVWWLILFNRKNVKQQFVDPMPG
                     EASPAVQTRRAPLAIVILAWFLIVSSGLALPFLLLEYRAPMMLFGHLLGGAAGAAYFV
                     AAWGLQLASGVGLLRLKRWSYPLTIGLQLFLLTSGIFTWLSPNFERAMRESLNSMGSF
                     PQGMDPEVYLRIGMRAGFVGFLFPVLILGILIYYRSRFLESAALATKMPPE"
     gene            3519..4142
                     /gene="lexA"
                     /locus_tag="VGR03_09670"
     CDS             3519..4142
                     /gene="lexA"
                     /locus_tag="VGR03_09670"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEV1994587.1"
                     /translation="MALTKRQKEVLDFLVSFETKHRYAPSFEEIGKGLKLTSLATVHK
                     HITTLERKGFIRRGYNQSRSIEILQLPRSVKEQVIERRVQELPLVGRIAAGRPLEALE
                     DRETISLGDFARGGNSFVLQVKGSSMIEDHILDGDFVVCEQTQVANPGDIVVALLGGE
                     EATLKRFYREPGGKVRLQPANSEMHPIFVSAADLKIQGRVIGVLRKY"
     gene            4537..4980
                     /locus_tag="VGR03_09675"
     CDS             4537..4980
                     /locus_tag="VGR03_09675"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEV1994588.1"
                     /translation="MSTDAMRSTGATSDRAVARLGASLHVKGEITGNEDLQIDGTVEG
                     LVQLDDRKLTVGPAAKLTADVIAREVVVYGTVKGNLRAKDRIEIKKDGSVHGDLTTAR
                     IMIEDGAYFKGSIEIDKPAEKDAEKNAFTRTASTSAAAAGATKNP"
     gene            5294..>7628
                     /locus_tag="VGR03_09680"
     CDS             5294..>7628
                     /locus_tag="VGR03_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV1994589.1"
                     /translation="MSRFQVSSLYSWCVFLLVICLFSPVFANAQSAQYATIVGQVVDQ
                     QNAVVPNATVTATNPATGFTRTTKSSSDGTYTISNLPPGTYNVKVDAGSSFTPGEAKA
                     FKLNVGDQRDLNFKLAIAGSQQTIEITSQAPLIETTKTDVSTSVTDLDMERLPTFAGI
                     GGVANDYAGLALTAPGVKLDTSSLTGDLIGPGSINNRANLYNVDGANITDQLVSGRDG
                     LGASVDEIQEFQVITNNYNAEYGQAGGLIINAVTKSGTNGVHGEGHMYFRGRNMRASD
                     PFYNIFVFQHAVPVGTVGPLGAPHCPTTDFVGGTLTKLDGCDRAPFHRKEGGFTIGGP
                     FVKNHLFWFASFENSRQSIPQVLTPPSGAIVVQEPVNNFLYSGKVDYKINDKHTLTAR
                     MWVDRQLFDNLIVQTGNNITPNSLTGLNFHVFGYNIGFVSALSSNLVNEARFAFVRQI
                     NALPDKTDLPGVQGPTFYTGAAFCCPQGGLQKRYQFIDNLTWTHGKHTFKTGFNISYY
                     PWYSLFQQFHFGQYTVTAANVPTSFTFAIGPGAVTSKDNIYGFYFQDTWKLTRKLTMN
                     AGLRYDYEAGAFKGGKLPGPGGTCFQGNGIIPACSSDKNNWQPRLGFTYAPWERTLFK
                     LSFAESTVLAFNNVVLDSLNFDGTTLNTINTTDPLVLAAFPNAPSPALLSGSAACPPS
                     CGRVRPISNHLKNPEIRSINFGIQHEFTKTLSAEIQYIGQFGFGLFGERDTNAAPVIA
                     DPTHPGFFYFGQRPDSRFTAIRTNENSRTSHYNGLLVS"
BASE COUNT         1594 a         2400 c         2092 g         1542 t
ORIGIN      
        1 gccggcgcac gcccgtggat gctgtggaac ccaaacgtgt tcatgtagta cgggaaccga
       61 cttgagcagc cgattccgct caccaccacg aacttctcgc ggggcacgcc cagctccggg
      121 aaaaccgatt gcaccgccgc aaggatcgcg tagtcgccgc agcccggaca ccagcgcact
      181 tcctgatcgg tttgaaaatc cttcttcgtc aaagtcggca gcgtcgccgt cgtcatctaa
      241 ctcactccca gcaactcttc gattttcact tccaattcgt tttgcttgaa cggccggccc
      301 tggatcttgt tcagtccaac ggcgtccacc aggtacttcg cccgcagcac ccacaggagc
      361 tggcccatgt tcagctccgg cacgagaact ttcttgtacc gcttcagcac ttcgccgaga
      421 ttgctcggca gcgggttcag gtgccgcagg tgcaaatggc cgatccggcg gccctttgcc
      481 cgctgcgcct tcagcgccac ggtgatggac ccgtacgttg agccccagcc cacgatgagg
      541 aggtcacctt cggcatctcc caccggcacc gcgtccggaa tatcctgtgc caccgccgcg
      601 accttcgccg cgcgaatgcg caccatgttc tcgtggttca tgggctcgta attcacgttg
      661 ccggttacgt cctgcttctc caggccgccg atgcggtgct ccaggcccgg agtcccggga
      721 atcgcccacg ggcgcgccag cgtttgcggg ttccgtttgt atgggaggaa tccttccggg
      781 ttcttctcaa agctcaccgg gatttcgggg atgtcgctaa gctggggaat gtgccacggc
      841 tcggcgccgt tcgccaggta cccgtcggac agtacgatta ccggcaccat atacttgatc
      901 gcgatccggc tcgcctcgat tgcgacccag aagcagtccc ccggcgttga cgccgccagc
      961 acgggaatcg gcgcctccga gttccgcccg aacagcgcct gcagcaggtc cgcctgttct
     1021 gtcttcgtcg gtaatcccgt ggagggcccg cctcgctgca cgttcacgat caccagcgga
     1081 atttccagca tcaccgccaa tcccatcgct tcggtcttga gcgccatccc ggggccggac
     1141 gtcgtcgtaa tcgccagcgc tcctgcatac gccgccccga tcgacgaagt aatcgccgca
     1201 atttcgtctt ccgcctggaa ggtcatcacg ccgaaatctt tgtactgcga gagctcatgc
     1261 agaatgtccg acgcgggcgt gatcgggtac gacccctgaa acagcgtcag tcccgctttc
     1321 tgcgctgccg tcacgaaacc cagcgccagc gcctggtttc cgctgaggct gcggtacacg
     1381 ccggggtcca gttgcgccgg cggaatctca tagctgacct ggaatgcttc ggtcgcttcg
     1441 cagtagctat accccgccct cagggccagc ttgttcgctt ccgccaggag cggcttgctc
     1501 ttgaactttt cttcgatcca ccggaccgtg gggtccatgg agcggttgta gagccagtag
     1561 cacatcccca gcgcaaagaa gttcttgcag cggtccgtcg ttttggcgtc gatgcccagg
     1621 tgctggagcg ccgcgcgcgt cagacgctgc agctccaccg ggaatagccg gaacttatcg
     1681 agcgaatggt cctcgagggg attcgccgtc atctgcgcct tgcgcagatc gttctctttg
     1741 aagctgtccc ggttgacgat caggatgccg ttggccttca ggtcgcccag attcatcttg
     1801 agcgcggcgg gattcatcgc gatcagcacg tccaccgcgt cgcccggcgt gtacacgtca
     1861 ctcgaggaaa aatgaagctg gtagccgctc accccgggca gcgtccccgc tggggcgcgg
     1921 atctcggccg gaaagtccgg aaacgtggcg atatcgttgc cgtaaagcgc gacggtgttt
     1981 gtgaactggc tgcctgtgat ctgcattccg tcgcccgaat cgcccgcaaa acggatgact
     2041 gcctgctcga tcacttcggg cttatgtttt gccagcggta gctcggtgac tgtggacatg
     2101 gtgacgaaaa tccccggcgc cttctagatt ctcctcatgc cggccagagg accgcggtct
     2161 tgtcggatgg gtggcccctg cccgattttg cggtcggcgc ggactccgaa aggcctcgca
     2221 acatcctagg ataccacatc gcgccgctcg ccgccgcgcg gaaacacttc gaaaccggcg
     2281 gcccgcagcc gtatgcttga caattcgtgt cgtgtcgctg cgcactccgg tgtgccctca
     2341 tcttcgtctc aatccatttc ttattcgggt ggcatcttcg tggcgagagc agcggattcg
     2401 aggaagcgac tccggtagta aatcagaatt ccgagaatca atacggggaa gaggaaaccc
     2461 acgaatccgg cccgcatgcc gatgcgcagg tagacctcgg ggtccatccc ttgcgggaaa
     2521 ctccccatcg aattcaacga ttcgcgcata gctcgttcaa agttgggact cagccatgtg
     2581 aagatcccgc tggtgagcaa gaacaactgc agcccaattg tgagtgggta gctccaacgc
     2641 ttcagccgca gcagcccgac tccagaagca agctgcaacc cccaggccgc aacgaaatac
     2701 gccgccccgg cggcgcctcc cagaagatgc ccaaacaaca tcatcggagc tctatactcc
     2761 agcaatagaa agggcagagc gaggcctgag gaaacgatca ggaaccaggc aaggatcacg
     2821 attgctagcg gcgcgcgacg agtttgcacc gcgggcgaag cttcacctgg cattggatcc
     2881 acaaattgtt gtttcacatt cttgcggttg aacaggatta gccaccagac agccattaga
     2941 gcaggcgccc cgtagaagat tgacaagaag acatgcatga acatcctaaa gtccggcccc
     3001 gccccgggcg gcgggggaaa cgtcaagttc aaagcgacaa gcagtccggt caccgcgaag
     3061 aatgccgtga ttcccgaaat aatcagaatc gagatccgcg cccagttccg cagccgtagc
     3121 agcccgatac cggtcacgat gacccaagca ttccatccga aaagaatccc aattccaatc
     3181 atccccgccg ttttcgcaag agccggcatc ggttgggcgc tggaaaaatc ttccggacgg
     3241 agcaagagaa cgccgatagc tcccaagccc gcgaaaagca gggcgagact tccacagatc
     3301 agagcaacga cagccgccgc cgtgaccgaa gcagaccgtt tcatcgcctt cccctcagga
     3361 tgtataaacc atctttaagt atatcgttgg gagcttcgcg cccacggcaa tcggatgaat
     3421 ttagtcgttg acgttcgctt tgcattcgcc tagactaaaa agcgaatacg gctccgcggc
     3481 tcggtcttga agctgagcct tgcgaaggga ggctccccat ggccctcacc aagcgacaga
     3541 aagaagtcct cgatttcctc gtctctttcg aaaccaagca ccgctacgcg ccgagcttcg
     3601 aagaaatcgg caagggcttg aagctcacgt cattggcgac agtgcacaaa cacatcacga
     3661 cgctggagag gaagggtttt atccgccgcg gctataacca gagccgctcc atcgagatct
     3721 tgcagttgcc ccgctccgtc aaggagcagg tcatcgagcg aagagtccag gagctgcctc
     3781 ttgttggccg catcgccgcc ggccgcccgt tagaagctct cgaggatcgc gaaaccatct
     3841 ctctcggcga cttcgctcgc ggtggaaatt cctttgtcct gcaggtcaag ggcagctcca
     3901 tgatcgaaga ccacatcttg gacggcgatt tcgtggtttg cgagcagacc caggttgcca
     3961 atcccgggga tatcgttgtc gccctgctcg gcggcgaaga agccaccctc aagcgtttct
     4021 atcgcgagcc cggcggcaag gtccgcctcc agcccgctaa ttccgagatg caccccattt
     4081 ttgtctccgc cgccgatttg aagattcaag gccgcgtcat cggcgtgctt cgcaaatact
     4141 gaggatttgc accggccatc ccctcctcac ggtctgttct cttggcccgc gcggcctggt
     4201 gccgttccaa cttgatgggc ctatgtgttc gtcgaaataa accgccatcg ccctgagggg
     4261 acggtcggtg cggtttagct ccaagaagtt ggaacggtac tagttactct ctggaacgcc
     4321 gcctgcactc gcgatttgca gcgccccctt cacaggaata aagttctcca caccccggct
     4381 gaaaacctgt tatcgttcgt gttgtcactc ggaggaccga tgtggagcaa taagaagccg
     4441 gatcttcggc aacctgcgga accggtgcca agaattccgc aaagttcaac gacaccgccg
     4501 aaatccgccc cggcggcctg ggagggaaca actgccatga gcactgatgc catgcgttca
     4561 acgggtgcca cgtccgaccg cgcggtagcg cggctgggcg cgagcctgca cgtgaaggga
     4621 gaaatcacgg gcaacgaaga tctccagatt gacgggacgg tggaaggact ggtgcagctc
     4681 gacgatcgca agctcaccgt cggtcccgca gccaagctga ccgcagacgt gattgcgcgc
     4741 gaagtggttg tctacggcac cgtgaaggga aatcttcgcg ccaaggaccg catcgagatc
     4801 aaaaaggacg ggtcggttca cggcgacctg accacggccc gcatcatgat cgaggacggc
     4861 gcctacttca aaggttccat cgaaattgac aagccggccg agaaagatgc ggagaaaaac
     4921 gctttcactc gaacggcctc cacttccgct gcggcagccg gcgcaaccaa gaacccgtaa
     4981 cgaaatcgca gccgcggcaa ggagttcctt ccgcgccgta ttgcgttcac cctctactta
     5041 agatcccccg cccgtcatcg cacggccgac ctgtactctc cgcatgacct caattccatg
     5101 cgaggaggcc aggagggggg cctagtcccg ggttctcgcc cgccccgggg ccaattgaaa
     5161 attctacttg cgctttgacc cgcgaaaacg ctagaatccg cagccgatac aggtaccggc
     5221 gtccgggctg tcagccttag gaagcgccgg taggggttga ctctcagggg ggttctacgg
     5281 aaaggagcgg ctcatgagcc gttttcaagt gtcctcactt tactcgtggt gtgtattctt
     5341 gctggtcatc tgtttgtttt cgcctgtctt cgcgaacgcg cagagcgcgc agtatgcaac
     5401 gattgtggga caagtcgttg atcagcagaa tgcggtcgtt ccgaatgcga cggtgacagc
     5461 caccaatcct gccaccggat ttacccgcac gacgaagtcc agttccgacg gcacctatac
     5521 gatttccaat ttgcctccgg gcacatacaa cgtaaaagtg gacgctggca gcagcttcac
     5581 tccgggtgaa gccaaggcct ttaagctgaa cgtcggcgac cagagagacc taaatttcaa
     5641 acttgcgatc gccggatccc agcagaccat tgaaattacg tctcaggcgc ctctcattga
     5701 gaccaccaag acggatgttt cgacgtctgt aactgacttg gacatggaga gactgcccac
     5761 ctttgccgga ataggcggcg tggcaaacga ttacgccgga cttgcgctga ccgctcccgg
     5821 agtaaagctg gataccagtt cgctgaccgg ggatctgatc ggtccgggat ccatcaataa
     5881 ccgcgccaac ctctacaacg tggacggtgc caatatcacc gatcagttgg tttccggccg
     5941 cgacggcctc ggcgcctcgg tggacgaaat ccaggaattc caagtcatca ccaacaatta
     6001 caacgccgag tacgggcagg ccggcggcct catcattaac gccgtcacca agtccggcac
     6061 caatggtgtc cacggcgaag gccacatgta ttttcgcgga cggaacatga gggcttccga
     6121 tcctttctat aacatttttg tatttcaaca tgcggtgccc gtcggcaccg tagggcccct
     6181 aggagcccct cactgcccga caaccgattt tgtcggaggg accttgacga aattggatgg
     6241 ctgcgatcgg gcaccattcc accgcaagga gggcggcttt accatcggtg gcccgtttgt
     6301 taaaaaccat ctgttctggt tcgccagctt cgagaacagc cgtcagtcca tccctcaagt
     6361 tttgacgcct ccttccggcg cgatagtcgt ccaggaaccc gtcaacaatt tcctctattc
     6421 cggcaaggtg gattacaaaa taaacgataa gcacacgctg accgctcgca tgtgggttga
     6481 ccggcagctc ttcgacaatc tgatcgtgca gactggcaac aatatcactc cgaactcgct
     6541 aaccggcctt aacttccacg tatttggtta caacataggc tttgtttccg cgctcagctc
     6601 aaacctggtg aatgaagccc gttttgcctt cgtccgccaa atcaacgcgc tgccggacaa
     6661 gaccgatctt ccgggcgtac agggtcccac cttttatacc ggcgccgcct tctgctgccc
     6721 tcagggcgga ttgcagaagc gttatcagtt cattgacaac ctcacctgga cgcacggtaa
     6781 gcacaccttc aagaccggct tcaacatcag ctactacccg tggtactcgc tcttccagca
     6841 gttccacttc ggccaatata cggttactgc ggccaatgta cccacgtcct tcaccttcgc
     6901 tattggtccc ggcgccgtga cttccaagga caacatctat ggcttctatt tccaggacac
     6961 ttggaagctg acccggaagc tcaccatgaa tgccggcctg cgctacgact atgaagccgg
     7021 tgcattcaag ggcggcaagc ttcccggacc gggtggcact tgtttccagg gcaacgggat
     7081 tattcccgcc tgctcctcgg acaagaacaa ctggcagccc cgcctgggct tcacctatgc
     7141 gccatgggag aggacgctgt ttaagctgtc tttcgccgag agtacggtgc tggcgttcaa
     7201 caacgtggtg cttgactcgt tgaactttga cggcaccacg ctcaacacca tcaacacgac
     7261 ggatccccta gtgctggcgg catttcccaa cgccccgagc ccggcgctgt tgagcgggtc
     7321 ggcggcctgc cctccctcgt gcggtcgcgt tcgtcccatc tccaaccatc tgaagaaccc
     7381 ggaaattcgc agcatcaact ttggaattca gcatgaattt accaaaacgt tgtcggcgga
     7441 aatccaatac atcgggcagt tcggcttcgg cttgttcggc gaacgggaca caaatgctgc
     7501 accggtcatt gccgacccga cgcatcccgg cttcttctat ttcggacaaa ggccggattc
     7561 ccggtttacg gctatcagga ctaacgagaa ctcccgcacc tcgcattaca acgggctgct
     7621 ggtaagcg
//
LOCUS       DASYVO010000294         1956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_121384, whole genome shotgun sequence.
ACCESSION   DASYVO010000294 DASYVO010000000
VERSION     DASYVO010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121384"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1825
                     /locus_tag="VGR03_09685"
     CDS             <1..1825
                     /locus_tag="VGR03_09685"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEV1994590.1"
                     /translation="VRAKQTRYGLRGPYVDRAPETLNKVVPIVMHGDAAFAGQGIWAE
                     TLNLADLKAYSVGGTIHIIVNNLLGFTTKPAQEHSSRFASDIAKRQSVPIFHVNAEDP
                     DAVVRIGKLAAEYRATFGSDAVVDIIGYRRHGHSEVDDPTITQPLLYERIKNHAPLWK
                     IYAEQTGIDSSLMVDAVRKEYEEEQTKARALRKIPHLRTLPSYWTPYQHGRYRPEYEV
                     DTGLTPEKLAEITDGLVRVPEGFHTHPKIVKLLEQRAEMGHGKRAVDYGFAELLAFGS
                     VLLEGNPVRLTGQDTERGTFSQRHSVLIDTQTEHNYLTLSHLSKQQAFCEIHNSSLSE
                     AGCLGFEYGFSRDYPEALVLWEAQFGDFANGAQVIIDQFISAGEDKWNLPAGLVMLLP
                     HGYEGQGPEHSSARLERFMQLAAEDNMQICQPSTAAQYFHMLRRQVRRPWRKPLIVFT
                     PKSMLRHPDASSTIEEFTRPRFLPLVPDREVQDAKRILIASGKVGHELRAERRRRKDT
                     STAIFFLDQLYPLPRTEIDAAIAEHPNAREIVWVQEEPGNMGAAGYVLPRFERIAKAR
                     GLNFRSVKRSASASPATGSAKAHEMEQKTLLALAFTTTSSD"
BASE COUNT          425 a          640 c          522 g          369 t
ORIGIN      
        1 ggtgcgcgcg aagcaaactc gctacggttt gcgcggccct tacgtcgacc gtgcgccgga
       61 aacactaaat aaggtcgtac ccatcgtgat gcacggcgat gcagctttcg cgggtcaggg
      121 catctgggca gaaacgctga atctcgcgga tctgaaggcc tatagcgtcg gcgggacaat
      181 ccacatcatc gtgaacaacc tgctcggctt tacgacgaag cctgcgcagg agcattcttc
      241 gcgcttcgct tccgatatcg ccaagcgcca gtccgtgccg atcttccacg tcaacgccga
      301 agacccggac gccgtcgtgc gcatcggaaa acttgccgcg gaatatcgcg cgacgttcgg
      361 cagcgacgcc gtcgtggaca tcatcggcta ccgccgccat ggccacagcg aagtcgacga
      421 tccgaccatc acgcagccgc tcctttacga gcgcatcaag aatcatgctc cgctctggaa
      481 aatttacgcc gaacagaccg gcatcgactc gtccctgatg gttgacgcag tccgcaagga
      541 atacgaagag gagcagacca aggcccgcgc actaagaaaa ataccgcacc ttcgcacatt
      601 gccgagctac tggacgccgt atcagcacgg ccgctacaga cctgaatatg aggtcgatac
      661 cggcctcact cctgaaaaac tcgcggaaat cacggacggc ctcgttcgcg tgcccgaagg
      721 gtttcacacc cacccgaaaa ttgtgaaact tctcgaacaa cgcgcggaaa tgggccacgg
      781 caagcgtgcc gtcgattacg gcttcgccga attgctggcg ttcggctccg tgcttctcga
      841 ggggaatccc gtgcgcctca ccggacagga caccgagcgt ggcaccttca gccagcgcca
      901 ctctgtgctc attgacacgc aaactgagca caactacctc actctctcgc atctttccaa
      961 acaacaggcc ttctgcgaga ttcacaactc ttctctttct gaggcaggct gcctcggttt
     1021 tgaatacgga ttcagccgcg actatcccga agcgcttgtg ctatgggaag cgcagttcgg
     1081 cgatttcgcc aacggcgccc aagtcatcat cgaccagttc atcagcgccg gtgaagacaa
     1141 gtggaacctg ccggccggcc tcgtcatgct tctgccgcac ggctacgagg gccaggggcc
     1201 ggagcattcc agcgcgcgcc tcgagcgctt catgcagctc gccgccgaag ataacatgca
     1261 gatttgccag ccttcgaccg ctgcgcagta ttttcacatg ctgcgccggc aggtgcgccg
     1321 cccgtggcgc aagccgctga tcgtttttac gccgaagagc atgctgcgcc atcccgacgc
     1381 cagttccact atcgaagaat tcactcgccc gcgttttctg ccgctcgttc ctgaccgcga
     1441 agtgcaggac gccaagcgca tcctgatcgc cagcggcaaa gttggacatg aacttcgtgc
     1501 cgaacgccgc cgccgtaagg acaccagcac ggcgattttc ttcctcgacc agctctaccc
     1561 gctgccgcgc acggaaatcg acgcagccat cgcggagcat cccaatgcgc gagaaatcgt
     1621 ttgggttcag gaagagcccg gcaacatggg cgcggctggc tatgttttgc cgcgattcga
     1681 acgcatagcg aaagccaggg ggctcaactt tcgctcggtc aagcgttccg ccagcgctag
     1741 ccctgccacc ggatccgcca aggcccatga gatggagcag aaaacgctcc tggccctggc
     1801 cttcacaacc acaagtagcg attagacttc ctgggcttta acactgcatt catattcatg
     1861 taatccccgc gtgatagcgc ccctgcgaca atcaatggtg ttacgctaaa tcccgggtga
     1921 tcaaaatctt cccaatgcct accgcaattc gccgtc
//
LOCUS       DASYVO010000295         3060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_275482, whole genome shotgun sequence.
ACCESSION   DASYVO010000295 DASYVO010000000
VERSION     DASYVO010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275482"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="VGR03_09690"
     CDS             <1..1372
                     /locus_tag="VGR03_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994591.1"
                     /translation="AASLPGSSGSSSGPLTPAMVMKPTTVAPDTSHPTPAVSPYDTQI
                     AADRSKLEEMSKPRPLWQQILRGAAATLPIVGPTMAYERNIKEKRRDQLISDIHNQQE
                     MGLRKQDFDIQQGNLQANREATQAYREGILGTKKTPQERQAEVEKQNRDPNDPAMQNY
                     ILSGDSAFLVPPTGKRETPQEREADVIARHMDPKDPNVGNYILTGDASLLRPDKPAAP
                     GSMDEQYLLALQSGDSKKAALIKQTMDANHPKQMTINPPSATGTWTLQEGPDGKSVLL
                     NSKTGETKAAPEGVVPRGTAERIRAERDKTMAPVEGAIDYATSYLGNKVYTGSGDEAL
                     QEKFFDLAKPTSGFRMTQPQMQMLQDSRGWMGGVAARLRHATTGTWFSDEQRSQIVQT
                     MKDLAAAKSKAFGGGGQTAPGANGQTAPRPAGIPDGYIHVQHTDGTTGWANPAAALKN
                     KWTTIR"
     gene            1321..>3060
                     /locus_tag="VGR03_09695"
     CDS             1321..>3060
                     /locus_tag="VGR03_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994592.1"
                     /translation="MGKPSGRAEEQVDDDTLMPEPQARQRAPDFIPDAAPDFIPDGGG
                     SPGATPYRPPGFEALKAQVATYPKEEPREGLLAGMSRRLIGTADPIHDVPAVLSDAIH
                     HPLEGLENAPGRITAALGAPQQLAALRRVPSALKSGQYGEALGSALEGVIPIGGPMAA
                     AIGEGLGSEHPEEGVRDAAALGYGALLGKAWPRVIDLAKDAAGAYSVPKPNALALGER
                     FTPEQIRATAKEQAIPMTRAEISGSPIATAVQKQVERGLLSSHLADQHATAVKGSLGE
                     WSNRLSDNLAPAATSEEAGGRIQTSLEGALAREKATAEANFKAVDGAAQGVTGSLAPA
                     KQAVQQALDESSFIRNAVPALDAKRSTTVLQGMLATKEAPSPIAGQPPIRVPMLPDNA
                     SFSQMQQLRSALLDEARHPDNVLNEQGQGRIRLAIGAVDQAMAEAAKNLNPQALDLWR
                     GANQHWADVHENFNNPRSPIYQILKSPDPNSAFQQLVPKSGGSPQVARQAMRYLGDDA
                     GTLKREFVQRLIDPKGNGQPNFGGVEQRLNAYKEGYLNELFQPEELDELRKFARLSRS
                     AGSLARMENPSNTA"
BASE COUNT          721 a          842 c          998 g          499 t
ORIGIN      
        1 ggccgcttcc ctgcccggat ctagtggatc ttcttccgga ccgcttacgc cggcgatggt
       61 gatgaaaccc acgactgtgg cgcctgacac ctcgcacccg acacctgcag tttcacctta
      121 cgacacgcag attgcggcgg atcgatcgaa gctcgaggag atgagcaagc cgcggccgtt
      181 gtggcaacag attctgcgtg gcgcggcggc gaccctgcca atcgtgggcc ctacgatggc
      241 ttacgaacga aatattaaag agaagcggcg cgaccaacta attagtgaca ttcacaacca
      301 gcaagagatg gggttgcgga aacaggattt cgatattcag caagggaacc tgcaggcgaa
      361 ccgtgaggcc acacaagctt atcgcgaagg gatactcggc acgaagaaga ctccgcagga
      421 gcgccaggcg gaggtagaaa aacagaaccg ggatcccaat gacccggcta tgcaaaatta
      481 tatattgagc ggggattctg ccttccttgt acctccaacc ggcaagcgtg aaaccccgca
      541 agagcgggag gcagatgtaa tcgcgcgaca catggacccg aaggacccga acgtagggaa
      601 ttacattctc accggtgatg cgtcgctctt gaggcccgac aagccggctg cgccgggcag
      661 catggacgaa caatatctcc tggccctgca gtccggagat tccaagaagg ctgcgcttat
      721 taagcaaacc atggatgcga atcatcctaa acagatgacc atcaatcctc cgagcgcgac
      781 aggtacgtgg acgcttcaag aagggccaga cggaaagtcg gtcttgctga acagcaaaac
      841 gggagagacg aaggcagcgc cggaaggcgt ggttccgaga gggacagcgg agcgcattcg
      901 agctgagcgc gacaagacga tggctcctgt tgaaggagcc atcgattatg cgacttcata
      961 cctgggcaac aaagtttaca cgggatctgg cgacgaagcg ttgcaggaga aattctttga
     1021 cctggcaaaa cctacgagcg ggtttcggat gacgcagccg cagatgcaaa tgctgcaaga
     1081 ttcaaggggc tggatgggag gcgtagcggc gcgcctccgc cacgcaacaa ccgggacctg
     1141 gttcagtgat gaacagcgca gccagattgt ccaaacgatg aaggatttag cagcggcaaa
     1201 gagtaaagcg tttggtggcg gcggccagac ggcgcccgga gcgaatggac agacggcgcc
     1261 gcggcctgcg gggattccag atggatacat tcatgtgcag cacacggatg gaacaaccgg
     1321 atgggcaaac ccagcggccg cgctgaagaa caagtggacg acgatacgct gatgcctgaa
     1381 cctcaagcca gacaacgcgc gcctgatttc atccctgatg ccgcgcctga ttttattcct
     1441 gatggtggcg gatcgcccgg cgctacgccg taccgtccac caggcttcga agcgttgaaa
     1501 gcgcaggtgg ccacttaccc gaaggaagag ccgcgggaag gactactggc gggaatgtcg
     1561 cgccggctga tcgggacggc tgatcccatt cacgatgtgc ctgcggtgct ttctgacgcg
     1621 atccatcatc cgctagaggg attagaaaac gctccgggga ggattacggc cgcgctgggt
     1681 gcgccacaac aactcgcagc gctgcggcgc gtgccaagcg cgttgaaatc ggggcaatac
     1741 ggtgaagcgc tgggatcggc gcttgaaggt gtgattccga tcggcggtcc tatggcggcg
     1801 gcaatcggag agggcctggg gtcagaacat ccggaagagg gtgtacgaga cgctgcggcg
     1861 ctcggatatg gcgcgctgct agggaaagcc tggccgcgcg tgatcgattt ggcgaaagac
     1921 gcagcgggag cttacagcgt gccgaaaccc aacgcacttg ccctgggcga acgatttact
     1981 ccggagcaaa ttcgcgctac ggccaaggag caagcgatcc cgatgacgcg ggcggagatt
     2041 tcgggctcgc cgatcgcgac ggccgtgcag aagcaagtgg agcgcgggct gctgagctca
     2101 cacctggcag atcagcacgc gacggcggtg aagggatcgc tgggcgagtg gtcaaaccgt
     2161 ttgtctgaca atctggcgcc ggcagcgact tcggaggaag ccggcgggcg aattcaaact
     2221 tctctcgaag gcgcgttggc tcgggagaaa gcaactgcgg aggccaattt caaggcggta
     2281 gacggcgccg cgcagggagt gaccggcagc ctcgcgccgg caaagcaggc ggtgcagcaa
     2341 gcgctcgatg aaagttcgtt tattcggaat gcggtgccgg cactcgatgc gaaacgaagc
     2401 accacggtgc tgcagggcat gttggcgaca aaagaagctc cgtcaccgat cgcggggcag
     2461 ccgccgattc gcgtgcccat gcttccggac aatgcgagct tcagccagat gcagcagctc
     2521 cgatcggcgc tcctggatga agcgcggcac ccggacaatg tgctgaacga gcaaggccag
     2581 ggcaggatca gactggctat tggggcggtt gaccaggcga tggcggaagc ggcaaagaat
     2641 ttaaatccgc aagcgctcga cttgtggcgc ggcgcgaacc agcattgggc agacgtccac
     2701 gagaatttca acaatcctcg ctcaccgatt taccagattc taaaatcgcc tgacccgaat
     2761 agcgcgtttc agcagctcgt tccgaagagt ggtggatcgc cacaggtggc gcgccaggcg
     2821 atgcgctacc tgggcgacga tgccgggacg ctgaagcgcg agttcgtgca acgcctgatc
     2881 gacccgaaag gcaacgggca gccaaacttt ggcggcgtag agcagcggct gaacgcttac
     2941 aaggaaggct acctgaacga gcttttccaa cctgaagaac tggatgagct tcggaagttc
     3001 gcgcggctct cgcggtctgc gggatcgctg gcgcggatgg agaacccgtc gaacacggct
//
LOCUS       DASYVO010000296        14183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_61902, whole genome shotgun sequence.
ACCESSION   DASYVO010000296 DASYVO010000000
VERSION     DASYVO010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 14183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14183
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61902"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VGR03_09700"
     CDS             complement(<1..737)
                     /locus_tag="VGR03_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEV1994593.1"
                     /translation="MALILVVDDEQSICELLEITFRKEGHKVEVASTVEAARRKLQSQ
                     IFDIVISDIRMPGSSGVELLQFTKEVSPECFFLLITGVPTLETAVAAINSGADRYVIK
                     DHNLIEQLRRAVRETADNLKWKKEAGYLRRELRRLTGLDNIIGQSPKMRAIFELIQTV
                     APQSSRVLITGESGTGKELVARAIHENSARTTAPFITINCGAFPETLLESELFGYVKG
                     AFTGANENRKGLFQASDGGTLFLDEIG"
     gene            complement(781..2394)
                     /locus_tag="VGR03_09705"
     CDS             complement(781..2394)
                     /locus_tag="VGR03_09705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1994594.1"
                     /translation="MEKSHTRREWLDWLTRVRLLMILLILAVALLWPQYGPATPLTRY
                     ILPILVFWTTLGVLYAVLVRQMPDARWHGPLQMGCDILVITSLVYVTGIQESYFISLY
                     LLVIIVASILFSRRMALITSGVCLLALAAATLLAYTGMIPQTYAVVPSPASLRSWFLS
                     HIFAFLAVAYLSGLLAQSLHRKDQELEEKREELLDLRNFTEDIIHSMRGGLITTDQEG
                     RILLLNRTGEEILGSQFENVRGKSLPELSPDFWLPGQKDASGNMLSRREIDFSTADGQ
                     QRFLGISVSPFHSRSVIAKGYVFNFQDLTELRRLEQEVATKDRMTALGRLSAAIAHEI
                     RQPLSAMAGAVKELGRLVPLEDDERHLVNIVSRESERLNLIITDFLNYSREKTYEFKE
                     ADLCALLDETLTLLEKKPELQAKYKVVRVFNGQVIRARVDVDRIKQVFWNLCDNAFRA
                     MPEGGTLTVRVEPLGFWLRISFRDTGVGLDAKRKAKIFEPLQSGFVGGTGLGLSIVYQ
                     IVQAHSGKIRVISEKDQGAEFVVELPRVA"
     gene            complement(2535..3746)
                     /locus_tag="VGR03_09710"
     CDS             complement(2535..3746)
                     /locus_tag="VGR03_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV1994595.1"
                     /translation="MPVFTYRGTNRTGASVSGELAAASKADLQNLLRRQQITASKMSE
                     KGKEFSLPTFGSGVTAKELAVFTRQFSVMIDAGLPLVQCLEILAGQQENKTFQKVLTG
                     TRSSVEGGTTLSVAMRQYPKIFDALFVNMVEAGETGGILDTILQRLASYIEKNVKLKR
                     AVKSALVYPVGVMTVAAGVITLLLWKVVPIFATLFTGLGVDLPLPTRIVIGMSNFIGS
                     IFGLLVLVFLVAVAVGLKLWYGTPKGRYAIDATLLKLPLVGIMLRKIAVARFTRTLGT
                     LISSGVPILEGLDITGRTAGNAVIERALQKVRKSLEEGKSLTDPLKETDVFPGMVTQM
                     IGVGEQTGAMDAMLQKIADFYEEEVDASVKDLLTALEPIMIVFLGLAVGGIVISMYMP
                     LFSLIGKLSSH"
     gene            complement(3810..4928)
                     /locus_tag="VGR03_09715"
     CDS             complement(3810..4928)
                     /locus_tag="VGR03_09715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEV1994596.1"
                     /translation="MPGNTLSELLKKMIEMGGSDLHIATNSAPRVRVHGRLRPLDMPP
                     LTAADTKSLAYSVLTDSQKHRFEENLELDFSFGLRNLARFRGNVFNQRGAVGAVFRAI
                     PWEIKGFEALGLPPVVKTLCDKPRGLVLVTGPTGSGKSTSLAAMLDKVNSEREEHMIT
                     VEDPIEFLHNHKKCLVNQREVHSDTLSFANSLRAALREDPDVVLIGEMRDLETIESAL
                     RIAETGHLTFATLHTNSAVSTINRIIDVFPAHQQPQIRAQLSMVIEGILCQALLPRAD
                     GRGRVMVMEVMVPTAAIRNLIREDKVHQIYSAMQSGTGQTGMQTFNQALANMYFQKSI
                     TLDMAMSRSSNQDELQDMITRGVSSVPSSGARVPVGRK"
     gene            complement(4949..6664)
                     /gene="pilB"
                     /locus_tag="VGR03_09720"
     CDS             complement(4949..6664)
                     /gene="pilB"
                     /locus_tag="VGR03_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEV1994597.1"
                     /translation="MSVRLGEILLKESLITQEQLQKAMEYQRTNGWKLGSCLTKLGYI
                     TDDDITGVLSRQYGVPSINLKSFEIDPSVTKLIPQDTATRYQVIPLSRVGAVLTIAMT
                     DPTNVFAMDDIKFMTGFNVEPVVASESAIAEAIARFYGAETNHEELSQMMKNLVDEDQ
                     DLELTADEEEVDAATQEKAAEQAPIIKLVNLILKDAVRRGASDIHVEPYELEMRVRFR
                     VDGVLQTVMNPPLRVKDAIINRMKIMSKLDIAEKRLPQDGRIMIKYKSEGKKKELDFR
                     VSTVPTLHGEKMVLRLLDKENLRLDMTKLGFEPESLKKFERNILRPYGMVLVTGPTGS
                     GKTNTLYSSVSRLNTAETNIMTAEDPVEFQLQGVNQVQMKEQIGLNFATALRAFLRQD
                     PNIILVGEIRDFETAEIGVKAALTGHLVLSTLHTNDAPSTISRLMNMGIEPFLVATSV
                     NLICAQRLVRRICEKCKEVLEVPEQALLDAGYTPGEASTTKIYHGKGCSTCNKGGYKG
                     RTGLYEVMEINDELRELILVGASALELKKKAIEQGMLTLRRSGLAKVAAGVTTLEEVL
                     RETVL"
     gene            6843..7637
                     /locus_tag="VGR03_09725"
     CDS             6843..7637
                     /locus_tag="VGR03_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994598.1"
                     /translation="MAPFASRPDFKARRPSSTSSPAHSPELPHAFRQFYDALYSALGP
                     QHWWPGRTPFEVILGAILTQNTSWANVERAMANLRKAKLLTPRAMEKISGPRLARLVR
                     PSGYFRQKARKLKAFMRFLRREYGGSLSRMFRTPVGELREKFLAVHGIGPETADSILL
                     YAGQQPAFVVDAYTRRILERHGHAAPRDSYEQIRARFESVLPRDAHLYNEYHALIVHT
                     GKNWCRTKNPRCDECPLAPFLPDRIRTVAAPDPGYFVEPVASLGAR"
     gene            7634..9859
                     /locus_tag="VGR03_09730"
     CDS             7634..9859
                     /locus_tag="VGR03_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1994599.1"
                     /translation="MKSSSRLRLIIGGILLTLLLAAVFTLGSLAVPFEPKNWAEISAL
                     YAVSTFITAALLVFGLILGRNVLRLWAERSKQQLGARFKTKMVIGAMAISLLPILFMF
                     IVSYSLLNRTLARWFPRPLEVAAQETRGLLLDFGKVQLQRLNELLVAASAPVASPGHG
                     TKPVRAEDLPPRAISLGADAAWLSQPDGSFSRIVRRGVYSIDSGQVIQSLATVPKRAR
                     QLPSGADVWLLGDEPYLTSTIPSAHDGAALVVARRLPQNFLTRYAEVEKQSRAYEQEK
                     QHLRALKTQMVLSLLLFTILLFTSVLWMALFLAKQVTVPIQALAEGTREVSAGNFSYQ
                     VPEQAQDELGVLVHSFNAMTMQLRDNRSQLDQFTRNLQQAVQELERRRQLMETILENI
                     PTGVVSLDTAGSVLRANAAMANIVGGAARSAQTLEELLDPDAARQVQNLMRRAQRMGV
                     ATREIELTAQGRVLHAAVTVSSLGPPRANSGYVIVLDDLTEVLRAQKSAAWQEVARRI
                     AHEITNPLTPIQLSAQRMIRFLERRDAQSPASDGPDSELADLVQDCSRLIEREVGTLS
                     SLVNEFTQFVRFPAAKLAPTDANQVVREALQVFAGRLEGITVNAAFEKDLPAIRGDGG
                     LLRSVIVNLIDNAAEALENAKVREIRVSTRAHPETELVEICVEDTGHGISAADKDRLF
                     LPDFSTKKRGTGLGLAIAARIIAEHNGTIRVEDNRPAGSRFRIELPVMESVAAQSAQR
                     A"
     gene            9880..11238
                     /locus_tag="VGR03_09735"
     CDS             9880..11238
                     /locus_tag="VGR03_09735"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV1994600.1"
                     /translation="MTTHSRILIVDDEAGIRESLSSILRDEGYHVSAAASGEEALELA
                     ARGDIDAVLLDIWLDGMDGLETLSRLQALPRPPAVIIISGHGNIETAVRATKLGAFDF
                     VEKPLTLEKIVVLVRNAVQQRQLAEENQSLRAELGQRYQIHGDSVPMKALRQQIAVTA
                     PTNGRVLIYGESGTGKELVARALHAASLRNKGPFVEVNCAAIPEELIESELFGHVKGS
                     FTGASEDKIGKFQKADGGTLFLDEVGDMSLRTQSKVLRVLEQQRFEPVGSSQTVGVDV
                     RVIAATNKNLELELTRGTFRQDLFYRLNVIPFFVPPLRDRREDIPTLTRYFLAEFSSA
                     YGRPSKEISDTAMEILLRYPWPGNVRELRNLVERLVIMSPQQHIEAHHLPPELFRGAA
                     ESPQRPYSTLHEARSAYEREFILRKLQENHWNMTQTASALGLERSHLYRKMKSLGIAA
                     PD"
     gene            complement(11426..12433)
                     /locus_tag="VGR03_09740"
     CDS             complement(11426..12433)
                     /locus_tag="VGR03_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994601.1"
                     /translation="MVTYVLGAGASRHAGYPLANELGNRLHDWVRQNWADEIIWKGYI
                     EALHKHYGGLADLELVLTELYERPAGSRATALSTTWCGNTLGGLRVAIPEFFNSLRDR
                     PLIGPDLYRSLARHKARNGEAILTFNYDLAVESAFRKEALWEIGDGYGFSLGVGITPT
                     SKVKVLKLHGSTNWLGILFDGNMGFSQYSRVYGPRPTLLRESDFRYLGYSNVVRDPHC
                     KGVSLTGGDPALVLPTLHKNFFHQTSSGREWEPFWNHIWGQAANVLHSSEKIVIIGYS
                     IPAADERARELLLGHSNSNAEISVFSGSRTDAICEAFRKSGFRIVKSSGKGRFEDFLS
                     N"
     gene            12566..13105
                     /locus_tag="VGR03_09745"
     CDS             12566..13105
                     /locus_tag="VGR03_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994602.1"
                     /translation="MTQLRKLVVIGAVATAFLQVNIEAQRKQKPPSIAFGTAEVSLGM
                     SVEQLQMNLSESARHIEFLSDKHTALIRVNNVPVPSGDEGQVTFFDGRVVYAAFHFPD
                     ARDAHQLAQEIAGAVENMDTKVCTVSNWSSHGTGGGLSQTILDCGSKSFNVMTTEILG
                     HNERYTHVEITIGAIPRPK"
     gene            complement(13290..13673)
                     /locus_tag="VGR03_09750"
     CDS             complement(13290..13673)
                     /locus_tag="VGR03_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1994603.1"
                     /translation="MKIKLASVFVDDQEKALKFYTEVLSFVKKADMPAGRFRWLTVVS
                     PEEAEGTQLVLEPNENPAAKTYQQAIHKEGIPATLLFTEDIQKECERMKKRGVVFRME
                     PTKMGPVTIAVFDDTCGNFMQLVQK"
     gene            complement(13670..13858)
                     /locus_tag="VGR03_09755"
     CDS             complement(13670..13858)
                     /locus_tag="VGR03_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994604.1"
                     /translation="MLLTTDTNLPYQQDFRGRKLAVVVLSKNRWKLIRPRLPEIAAAI
                     ASAKPGTCSVVEIPEGSS"
     gene            complement(14002..>14183)
                     /locus_tag="VGR03_09760"
     CDS             complement(14002..>14183)
                     /locus_tag="VGR03_09760"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEV1994605.1"
                     /translation="SGAWVFRGTRIPVAAVFENLEDGLAMGEIVRMFDGLTQEQVKTV
                     LDFAARSLSSPAHQR"
BASE COUNT         2888 a         4397 c         3943 g         2955 t
ORIGIN      
        1 tcgccgattt cgtccaggaa tagcgtcccc ccgtccgagg cctgaaagag tcccttgcgg
       61 ttttcgttcg ctcccgtaaa cgcgcctttc acgtaaccga atagctccga ctcaaggagc
      121 gtttccggaa aagcgccgca attgatggtg atgaagggag ctgtcgtgcg ggcgctgttc
      181 tcatggatgg cgcgcgcgac caactccttc cccgtgccgc tttccccggt gatcaaaacg
      241 cggctcgact gcggagcgac cgtttgaatc aattcgaaga tggcgcgcat cttcgggctc
      301 tgtccgatga tattgtccag tcccgtgagg cgccgcaact cccgccgcag gtatcccgct
      361 tctttcttcc atttcaaatt gtccgccgtc tctcggacgg cccgccgcaa ttgctcgatc
      421 aagttgtgat ctttaatcac gtaccggtca gcgccggaat tgatggccgc aacggcggtt
      481 tccagcgtgg ggacacccgt gatgagaaga aagaagcact cgggcgaaac ttctttggtg
      541 aactggagca actccacccc gctgctgccg ggcatccgga tatccgaaat cacgatgtcg
      601 aagatctgag actggagctt gcgccgcgca gcttccaccg tggaagccac ctccaccttg
      661 tggccttcct tgcggaaggt aatttccagc agttcgcaga tggactgctc gtcatccacc
      721 acgagaataa gggccatctc ccgttcactc ccccggattc acacggccag gcgcaccgct
      781 ctaagcaacc cgcggcaact cgacgacgaa ctctgcaccc tgatcttttt ccgaaatcac
      841 gcgaatcttc ccgctgtggg cctgcacgat ctggtagacg atggaaagac caagcccggt
      901 tccacccacg aagcccgatt gcagcggttc gaagatcttc gccttccgtt ttgcgtcgag
      961 gccgacgccc gtatctcgaa aagaaatccg cagccagaag ccgagaggtt ccacccgcac
     1021 ggtcaaggtg cctccttccg gcatggcccg aaacgcgttg tcacaaaggt tccagaagac
     1081 ctgtttgatg cgatccacgt ccacgcgagc gcggatcacc tggccgttga agacgcgcac
     1141 gactttgtat ttcgcctgca gctccggttt cttctcgagc aacgtcaggg tctcatcgag
     1201 caaagcacac aggtccgcct ctttgaactc gtaggtcttc tcgcgcgaat agttcaaaaa
     1261 atccgtgatg atgagattca gccgctccga ttcccggctg acgatgttga ccaggtgccg
     1321 ctcatcatct tcgagcggca ccaggcgccc cagctccttc accgcgcccg ccatcgccga
     1381 gagcggctgg cgaatttcat gcgcgatggc tgcggaaagg cggcccagcg ccgtcatcct
     1441 gtctttggtg gcgacttcct gctccagacg gcggagctcg gtcaagtcct gaaagttgaa
     1501 cacatagccc ttcgcgataa cgctacgcga gtgaaaaggc gagacggaaa tgcccagaaa
     1561 gcgctgctgt ccatccgcgg tactgaagtc gatttcccgc cgggacaaca tgttcccgct
     1621 tgcgtctttt tgaccgggaa gccagaaatc gggggagagc tccggcagac tcttgccgcg
     1681 aacattttca aactgggagc cgaggatttc ctcgccggtc cgattcagca gcagtatccg
     1741 gccttcctga tccgtggtga tcagcccccc tcgcatggaa tgaataatgt cctcggtaaa
     1801 attccggagg tccagcaatt cttcccgctt ctcctcgagc tcctggtcct tccgatgaag
     1861 ggactgggcg agcaagccgg aaagataggc caccgcaaga aacgcgaaga tatggctcag
     1921 aaaccaggaa cgcaggctcg caggggaagg cacgaccgca tacgtttgcg ggatcattcc
     1981 tgtataagca aggagggtcg ccgccgccaa tgcgagcagg catactcccg aagtgatgag
     2041 cgccatgcgg cggctgaaca gaatgctggc gacgatgatc accaacagat acagcgagat
     2101 gaaatagctt tcctggatcc cggtcacata cacgagggag gtaatgacca ggatgtcgca
     2161 acccatctgc aggggaccat gccagcgcgc atccggcatt tgccgaacca gaacggcgta
     2221 aaggactccc agggtagtcc agaacacgag gatggggagg atgtagcgcg tgagcggcgt
     2281 tgccggtccg tactgcggcc aaagaagcgc cacggcaaga ataagcagga tcatcaacag
     2341 ccggacccgc gtcagccagt ccagccattc ccgcctggtg tgtgattttt ccatttgcgc
     2401 agccaaccaa cggctcccgc cccgcgggcc cgtggtgagc cctgatgacg cccgcgggaa
     2461 aaccgaaccg ccggggtgca cattccgggc cccagggagg accggagcat ccgggggcag
     2521 ctggctcgat tcgcctagtg tgagctcagc ttgccgatca gcgagaacag aggcatgtac
     2581 atcgagatca cgatgccgcc gacggccagt ccgaggaaca caatcataat gggttccagc
     2641 gctgtcagga ggtctttcac cgaagcgtca acttcctcct catagaaatc ggcgatcttc
     2701 tgcaacatcg catccatggc tccggtctgt tcaccgaccc cgatcatctg cgtcaccatc
     2761 ccgggaaaga cgtccgtttc ctttagcgga tcggtgagcg acttgccttc ctcgagggac
     2821 ttgcgcacct tctgaagggc tctttcgatc accgcattcc ctgcagtccg gcccgtaatg
     2881 tcaaggccct ccagaatcgg aacgccgctt gaaatcaagg tgcccagggt gcgagtgaac
     2941 cgggccaccg cgattttgcg gagcatgatt cccaccagcg gcagcttcag aagagtggca
     3001 tcaatcgcgt agcgcccttt cggagtgcca taccataatt taaggcccac ggcgactgcc
     3061 accaggaaaa cgagaaccag cagcccaaat atgctcccga tgaaattgct cattccaatt
     3121 acgattcgag tggggagggg caaatccacg ccgagcccgg tgaaaagggt ggcgaagatc
     3181 gggacgactt tccacagcag cagggtaatc acgccggccg ctacagtcat gacgccgacc
     3241 ggatagacca gggctgattt cacggcgcgc ttcagcttga cgttcttttc gatataactg
     3301 gcgagacgct gcagaattgt gtccagaata ccgccggtct cgccggcctc gaccatattc
     3361 acaaacagtg cgtcgaagat cttgggatac tgcctcatag cgaccgaaag cgttgtcccg
     3421 ccttccacgg aggaccgcgt gcccgtcagg accttctgga aagtcttgtt ctcctgctgc
     3481 cccgcgagaa tctccaggca ttgcacgaga gggaggcctg cgtcgatcat gaccgagaat
     3541 tgccgggtga acaccgcgag ctccttcgcg gtcacaccgg agccgaaggt cggaaggctg
     3601 aactctttcc ccttttccga catttttgag gccgtgatct gctggcggcg cagcaggttc
     3661 tgtaagtcgg ccttgctcgc cgccgccagt tccccgctga cgcttgctcc ggtgcgattt
     3721 gtgccacgat aggtgaatac tggcatgtgc gtaaccctcc tcgatgaatc cgaaatatgc
     3781 gccctgactg ctcagggcgg aaaatcagcc tacttccggc ctaccggaac tctagccccg
     3841 ctggacggca cgctggaaac gccgcgagtg atcatatcct gcaattcatc ctggttggag
     3901 gaacgcgaca tggccatgtc caacgtgatc gacttctgaa aatacatatt ggccaatgcc
     3961 tggttgaaag tctgcatgcc ggtctgaccg gttcctgact gcattgccga gtaaatctgg
     4021 tgaaccttgt cttcgcggat caaattccgg atggccgcgg tcggcaccat gacttccatc
     4081 accatcaccc ggccgcgccc gtcggcgcgc gggaggagcg cctgacagag aatcccctcg
     4141 atcaccatgg agagctgcgc ccgaatctgc ggctgctggt gcgcggggaa gacgtcaatg
     4201 atgcggttga tggtggaaac cgcagaattc gtgtgcagcg tggcgaaggt caggtggccg
     4261 gtctccgcaa tgcgcagcgc ggactcgatc gtctcgaggt cgcgcatctc gccgatgagc
     4321 acgacgtcag ggtcttcgcg aagtgcggcg cgcagagaat tggcgaacga aagcgtatcc
     4381 gaatgcactt cgcgctggtt gaccaggcac ttcttgtggt tgtgcagaaa ctcgatcggg
     4441 tcctcgaccg tgatcatgtg ctcttcgcgc tcgctgttga ccttgtcgag catggcagcc
     4501 agcgatgtgg actttccgga gcccgtcggt ccggtaacca gaaccagacc gcgcggcttg
     4561 tcacagagag tcttcaccac gggaggcagg cccagggctt cgaacccctt gatctcccag
     4621 ggtatggcgc ggaatacggc gcctaccgcc ccgcgctgat taaagacgtt gccgcggaag
     4681 cgggcaagat tccgcaatcc gaaagagaag tcgagttcga ggttttcttc aaagcggtgc
     4741 ttttgcgagt ctgtcagcac actgtaggcg agcgactttg tatctgccgc ggtcagcggc
     4801 ggcatgtcca gcgggcgcaa gcggccgtgg acccgcacgc gtggtgcgct gtttgtggcg
     4861 atgtgcaggt cgctcccgcc catctctatc atctttttca gcaactcact gagtgtgttt
     4921 ccaggcatgg tccgaccttc cttagcttct ataaaaccgt ctcgcgcagg acttcctcca
     4981 gcgtggtgac tcccgcggct actttggcca gcccgctccg ccgcagagtg agcattccct
     5041 gctcgatggc ttttttcttc agttccagtg ccgaagcacc gacgagaatc agctctctca
     5101 attcgtcgtt gatctccatc acctcgtaaa gcccggtgcg ccccttataa ccgcccttgt
     5161 tgcatgtgct gcagcccttt ccatgataaa ttttcgtcgt gctggcctct ccgggagtgt
     5221 acccggcatc gagtaaggcc tgttccggca cctccaaaac ttccttgcat ttttcacaga
     5281 tgcgccggac caggcgttgt gcgcagatca ggttgaccga tgtggccaca agaaacggct
     5341 cgattcccat gttcatcagg cggctgatgg tggaaggcgc gtcgttggtg tgcagcgtgg
     5401 aaagcaccag gtggccggtc agcgccgcct tcactccgat ctccgcggtc tcaaagtcgc
     5461 ggatctcgcc gacgagtatg atgttcgggt cctgccggag aaaagcgcga agtgcggtgg
     5521 cgaaattcag cccaatctgc tccttcatct gtacctgatt cacaccctgt aattggaact
     5581 ccacgggatc ttcggcggtc atgatgttgg tttcggccgt gttcagccgt gaaaccgagg
     5641 agtacagcgt gttcgttttg ccggaacccg tcggaccggt tacgagtacc atcccgtagg
     5701 gcctcaggat gttgcgttcg aacttcttca gggactccgg ctcgaagccg agcttggtca
     5761 tgtcgagccg caggttttcc ttgtccagca accgaagaac catcttttct ccatgaagcg
     5821 tcggaacagt ggaaacacgg aagtccaact ccttcttctt cccttccgac ttgtacttga
     5881 tcatgatgcg gccgtcttga ggcaatcgtt tttccgcgat gtccagcttg gacatgattt
     5941 tcatgcggtt gatgatcgca tccttcacac gcagcggagg attcatgacc gtttgcaaga
     6001 caccgtcgac gcgaaagcgc acgcgcatct cgagttcata gggctcgacg tgaatgtcgc
     6061 tggcgccgcg cctcaccgca tctttcagaa tgaggttgac caacttgatg atgggtgcct
     6121 gctcggcggc cttttcctgc gtcgcggcgt cgacttcttc ctcatccgcc gtcaactcca
     6181 ggtcctggtc ttcatcgacc aggtttttca tcatttggct cagttcttca tgattcgtct
     6241 cggctccgta gaagcgcgca atcgcctcgg cgatggcaga ctccgaggcc accacgggct
     6301 ccacgttgaa tcccgtcatg aacttgatgt cgtccattgc gaatacgttt gtgggatcgg
     6361 tcatcgcaat cgtgaggacg gcgccgactc gggagagcgg tatcacctga taacgcgtgg
     6421 ccgtgtcctg gggaatcagc ttggtgacgc tcggatcgat ctcgaaggac tttaggttga
     6481 tggagggaac gccgtactgc cgggacaata ccccggtgat gtcgtcgtcg gtgatatagc
     6541 ccagcttcgt caggcagctg ccgagtttcc agccgtttgt gcgctggtac tccatggcct
     6601 tctgtagctg ttcctgtgtg atcaggcttt ctttaagcag tatttcgcca agccggacgg
     6661 acatccgagc gctccaggga aaataaacct aggctaacat tagcagaggg gacctgggta
     6721 agacggacaa attctgttcc cgttttgaag tcgctaatac ctgtccctgg gaacaggtag
     6781 ccccggtaag gtcgggttga attcttgact tgccgatatg cggcggctat cttcgagtag
     6841 agatggcccc cttcgcatcc aggcctgatt tcaaagcacg ccggccgagt agcacctcct
     6901 cccccgctca ctcgccggag cttccccatg cgtttcggca attctatgat gcgctctaca
     6961 gcgctcttgg cccgcagcac tggtggcccg gacgtacccc gttcgaggtc atccttgggg
     7021 ccatcctgac gcaaaacacc tcctgggcaa acgtcgaacg agcgatggcg aatctccgca
     7081 aggcaaagct cctgacaccg cgagccatgg agaagatctc cgggcccagg ctcgccaggc
     7141 tggtgcgccc ctccggttat ttccgccaga aggcccggaa gctaaaggcc tttatgcgct
     7201 ttttgcggcg ggagtatggc ggttcgctct cccgaatgtt tcgcacaccg gtcggcgagc
     7261 ttcgcgaaaa gtttcttgcc gtccacggca tcgggccgga gacggcggat tccatccttc
     7321 tttacgccgg tcaacagccg gctttcgtcg ttgacgccta cacgcggaga atcctcgagc
     7381 gtcacggcca tgccgctccg cgcgactcgt acgaacagat tcgcgcgcga ttcgagagcg
     7441 tcctccccag ggacgcgcat ctctataacg aataccacgc gctgatcgtt cacaccggca
     7501 agaactggtg ccggacaaaa aacccccggt gcgacgagtg cccgcttgct ccctttcttc
     7561 cggatcgcat tagaacggtg gccgcaccgg accctggcta cttcgtcgag cccgtggcct
     7621 ccctgggagc ccgatgaaat ccagctcacg cttgcgcttg atcatcgggg gcatcctcct
     7681 cacgctcctc ctggcggcgg tatttaccct cggctcgctg gccgttcctt tcgagcccaa
     7741 gaactgggcg gaaatctccg ccctctatgc ggtttcaacg tttatcaccg cggcgcttct
     7801 ggtcttcggc ttgattctgg ggcgcaacgt tttgcggctc tgggccgagc gcagcaaaca
     7861 gcagttgggc gcgcgcttca agaccaaaat ggtcatcgga gcaatggcca tttcacttct
     7921 ccccattctc ttcatgttta ttgtgagtta ctcgcttcta aaccgcacgc tggcgcgctg
     7981 gttccctcgt cccctggaag tcgcagccca ggaaacccgg ggcctgctcc tcgatttcgg
     8041 taaggtccag ttgcaaaggc tcaacgagct tctcgtagcg gcctccgcgc ccgtcgcttc
     8101 gccgggccac ggcacgaagc ccgtgcgagc cgaggatttg cctcctcgcg cgatctctct
     8161 cggcgcggac gctgcctggc tctcccagcc ggacggatcg ttctcgagga tcgtccgccg
     8221 cggcgtctat tcgattgact ctgggcaggt gatccaatcc ctggccacgg tcccgaaacg
     8281 tgcccgccag cttcccagcg gcgccgacgt atggctgctc ggtgacgaac cctaccttac
     8341 cagcacaatt ccatccgctc acgacggcgc cgcactcgtg gtggcgcgcc ggctcccgca
     8401 aaatttcctc acgcgctatg cggaggtgga aaaacagtcc cgcgcctacg aacaggaaaa
     8461 acagcacctt cgcgcgttga aaacacaaat ggtcctcagc ctcctcctgt ttacgatcct
     8521 tcttttcacc tccgtcctct ggatggctct cttcctggcg aaacaggtta ccgtgccgat
     8581 tcaagccctc gccgaaggca cacgcgaggt ttcggccgga aacttttcct accaggttcc
     8641 cgagcaagcc caggacgagc tgggcgtcct cgtgcattcg tttaacgcca tgaccatgca
     8701 attgcgcgac aaccgttctc agcttgacca attcacccgc aatcttcagc aggccgttca
     8761 ggagctcgaa cgccggcgcc aattgatgga aaccattctt gaaaacattc cgaccggcgt
     8821 cgtctcgctg gacacagcag gctccgtgct ccgcgccaat gccgccatgg ccaacatcgt
     8881 gggtggcgcc gcgcgcagcg cgcaaacatt ggaagagctg ctcgaccccg acgccgctcg
     8941 gcaggtgcag aatctgatgc gccgcgcgca gcgcatggga gttgccacgc gggaaatcga
     9001 actcacggcc cagggccgcg tcttgcacgc cgccgtaacg gtcagttccc tggggccgcc
     9061 ccgcgccaac agcggctatg tgattgtcct cgacgacctc acggaagtgc tccgcgccca
     9121 gaaatccgcc gcctggcagg aggtggccag gcgcatcgcc cacgaaatca cgaatccgct
     9181 gacccccatt caattgtcgg cacaacgcat gattcgcttt ctggaaaggc gtgatgcgca
     9241 atccccggcc tctgacggcc ccgattccga actcgccgac ctggtgcagg attgttcgcg
     9301 attgatcgag cgggaagtcg gcaccctctc ctctcttgtc aatgagttca cgcagttcgt
     9361 ccgcttccct gccgcgaagc ttgcgcccac cgatgccaat caggtcgtcc gtgaagcgtt
     9421 gcaggtcttc gccggccggc tggaggggat cacggtgaat gcggccttcg aaaaagatct
     9481 cccggcaatc cggggcgatg gcggactgct ccgcagcgtc atcgtcaatc tcattgacaa
     9541 cgccgcggag gccctggaaa acgcgaaggt cagggagatt cgcgtctcca cccgcgccca
     9601 cccggagacg gagctggtgg agatctgcgt cgaagatacc gggcatggca tttccgccgc
     9661 ggacaaggat cgtctgtttc tcccggactt ctcgaccaag aaacgcggca cgggccttgg
     9721 cctggccatc gccgcgcgca tcatcgccga acacaacggc acgatccgcg tcgaggacaa
     9781 tcgcccggcg ggatcgcggt tcaggataga attaccagtc atggaaagcg ttgccgcgca
     9841 atccgcccag cgcgcatgag acggagctag ctccacccga tgacgactca ttcccgcatc
     9901 ctgatcgtgg acgacgaagc cggcatccgc gagtccctct cgtccattct ccgcgacgag
     9961 ggctatcacg tctcggctgc cgcttccggt gaagaagccc tcgagcttgc ggcgcgcggc
    10021 gacatcgatg ccgtgctctt ggacatctgg ctcgatggca tggacggcct cgaaacgctc
    10081 agccggctgc aagcccttcc ccggccgccg gcggtgatca tcatctcggg acacgggaat
    10141 atcgagaccg ccgtacgagc caccaagctg ggcgcgttcg atttcgttga gaagccgctc
    10201 actctcgaaa agattgtcgt cctggtccgc aacgccgtgc agcagcggca gcttgcggaa
    10261 gaaaaccagt ccctccgagc ggaattgggg cagcgctacc agattcacgg cgacagcgtg
    10321 cccatgaagg cgctgcggca gcagattgcc gtcaccgctc cgaccaatgg ccgcgtcctc
    10381 atctatggcg agagcggcac gggcaaggag ttggtagcgc gcgcgctgca cgcggccagc
    10441 ctgcgcaaca aaggcccctt cgtcgaagtc aattgcgccg ccatcccgga agaactcatc
    10501 gagtccgaac tgttcggcca cgtcaagggc agcttcactg gcgcgagcga ggacaagatc
    10561 ggcaagttcc agaaggccga cggcggcact ctcttcctcg acgaagtcgg cgacatgagc
    10621 ctccgcacgc aatccaaagt cctgcgcgtc ctcgaacagc agcgctttga gcctgtcggt
    10681 tccagccaga cggtcggcgt tgacgttcgc gtcatcgccg ccaccaacaa aaacctcgag
    10741 ctggagctca cccgcggaac tttccgccag gacctcttct atcgcctgaa tgtgattccc
    10801 ttcttcgttc ccccgctgcg cgaccgccgc gaagacatcc ccacgctcac gcgctacttt
    10861 ctcgcggaat tcagttcggc ctacggccgt cccagcaagg aaatcagcga cacggcaatg
    10921 gaaattttgc tgcgctatcc ctggccgggg aatgtgcgcg aactccgaaa cctcgtcgag
    10981 cgcctggtca tcatgtcccc gcagcagcac atcgaagcgc accatctccc gcccgagctt
    11041 ttccgcggcg ccgcggaaag cccgcagcgg ccctattcca cgctgcacga agcgcgcagc
    11101 gcctacgagc gcgaattcat tctccggaaa ttgcaggaaa accactggaa catgacgcag
    11161 accgccagcg ccctcggcct cgagcgctcc cacctctacc gcaaaatgaa gtccctcggc
    11221 atcgccgccc cggattagcg tcctggccta atcctgcgcc tcgcccttgt agcgccgccc
    11281 ttcagggcgg catctggcag cgccaccaag cgcggtcatc ccgaccgaag tccggaccgg
    11341 agaccggcac gcagcgaagg gatcatggcg caatcttcta tctcggcatg aagtgatggc
    11401 gagaaaaatc agcagcgtag cagggtcaat tcgacagaaa gtcctcaaag cgaccttttc
    11461 cagaagactt gacgatccga aaaccactct tgcggaacgc ctcgcagatg gcgtctgttc
    11521 tcgatccaga aaacacagaa atttcggcat tggaatttga gtgccccaac aagagttctc
    11581 tcgctcgttc atctgcagcg ggaatgctgt agccaatgat gacgattttc tctgaagaat
    11641 gcaggacatt tgcggcttgt ccccaaatat ggttccaaaa aggttcccac tcgcggccag
    11701 atgaagtttg atggaagaag ttcttatgaa gtgtcggcag gactagtgcg gggtccccac
    11761 ccgtcagtga gacgcctttg caatgagggt ctcggacgac attcgagtag ccaagatacc
    11821 tgaagtcgga ttcacggagg agagtcggcc ttggaccata cacgcgtgag tattgtgaga
    11881 atcccatgtt tccatcaaag agtatgccca gccagttcgt gctgccgtgc agtttcaaca
    11941 ccttcacctt ggatgtcgga gttatcccaa cccctaatga aaacccgtac ccgtcgccga
    12001 tttcccacaa cgcttccttt ctgaacgcac tttcaaccgc taggtcatag ttgaatgtga
    12061 gaatcgcctc gccgtttcga gccttgtggc gggcaagcga tctgtagaga tcgggtccaa
    12121 ttagcggcct atctcgcaga ctattaaaga attccgggat tgcaacgcgc aggccgccaa
    12181 gggtattgcc acaccacgtc gtgcttaacg ctgtcgcccg cgagccagcg ggacgttcgt
    12241 aaagctctgt taagaccaat tccagatccg ccagcccgcc atagtgcttg tgtagcgcct
    12301 cgatatagcc tttccagatt atctcgtccg cccagttttg gcggacccag tcatgaaggc
    12361 gattccctag ctcgttagcg agcgggtaac cagcgtggcg ggatgcgcca gcacctagga
    12421 cgtaggtaac catgtcaaag acataagacc tctcctcaga atctaccatc tttcaactgg
    12481 cgtcgcggcc cgtggattgg ttgacgccgg ccccgtgaga aaagttcccg acgcatgcta
    12541 gaatccgccg agcggagaaa tcaacatgac tcagcttagg aagttagttg taattggcgc
    12601 agtggctact gctttcctcc aggtcaacat tgaagcacag cgcaagcaaa agccgccgtc
    12661 catcgccttc ggcacagccg aggtttcgct tggaatgtcg gtagaacaac tccaaatgaa
    12721 tttgtccgag agtgctcggc acattgaatt cctgagtgat aagcacaccg cacttatccg
    12781 tgtaaataac gttcccgtgc ctagcggcga cgagggacag gttacgtttt tcgatggacg
    12841 cgttgtttat gcagcgtttc attttcctga cgcccgtgac gcccaccagt tggcccaaga
    12901 gattgccgga gccgtggaga acatggatac gaaagtttgt accgtttcaa actggagcag
    12961 tcacggaacg ggcggcgggc tgtcacaaac catcctcgat tgcggctcaa agagtttcaa
    13021 cgtgatgacc acggagattt tgggacacaa cgagcgctat actcatgtag aaataacgat
    13081 tggcgcgata ccgcgcccga agtaggtggg cgctgacggt gggtggcctg ctcgatttaa
    13141 ctagaatagt ttcttgacgg ccctgcacga tgagaggatt ccagttccat aagtcaggct
    13201 cacgaaacaa gacgacggtg ccccaccctt tcacgagaaa gggcttgccc tgaactcgcg
    13261 aagggtggga aattcttctc acgattccct tatttctgca cgagctgcat gaaatttccg
    13321 caagtatcat caaacacggc gatggttacc ggccccattt ttgtcggctc catgcggaac
    13381 accactcccc gctttttcat tctttcgcac tctttttgaa tgtcctcggt gaacaataag
    13441 gtcgcaggaa ttccttcctt atgaatcgcc tgctggtaag tcttcgcggc cgggttctcg
    13501 ttgggctcca gaaccaactg agtgccctcc gcctcctcgg gcgagacaac ggtcagccat
    13561 cggaacctcc ccgcgggcat gtcggctttc ttaacaaagc tcaaaacctc cgtataaaac
    13621 ttcagggcct tttcctgatc atccacgaat acgctggcaa gtttaatctt caacttgatc
    13681 cctcgggtat ttcgaccacc gagcaggttc cgggctttgc ggacgcgatc gccgcagcaa
    13741 tctccggaag cctgggacgg atcagcttcc acctgttctt gctcagaacg acaaccgcca
    13801 gcttgcggcc tctgaagtct tgctggtagg gaagattcgt atctgttgtc agcaacacgt
    13861 tgaatcctcc ttcttctgct gcccgaagca gttcaccgtt cgcaagagtg gtccatcctt
    13921 gttctcgtgc agtcttcacc gtgtgttccg gcaatgaatc ccggattccg acgggagttc
    13981 cttggtcaaa caggatgagc attaacgctg gtgggcgggc gaggagaggc tccgcgccgc
    14041 gaagtcaagt acggttttca cctgttcttg cgtcaggccg tcaaacatgc gcacgatttc
    14101 gcccatggcg agcccgtcct cgaggttttc aaacactgcg gcaacgggga tccgcgtacc
    14161 ccgaaaaacc caggcaccgc tca
//
LOCUS       DASYVO010000297        21118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_275489, whole genome shotgun sequence.
ACCESSION   DASYVO010000297 DASYVO010000000
VERSION     DASYVO010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 21118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21118
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275489"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..689
                     /locus_tag="VGR03_09765"
     CDS             180..689
                     /locus_tag="VGR03_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994606.1"
                     /translation="MAIHKWKFHFAACPVLLSVMLASVLFSSDNIVRGQTGPQSFDEA
                     VHAKHKRFVEYHQTFNSFAQSGLGSDEFHVAMSLTTIASRTGDYLWSVHILLEIYGGL
                     SCEEDRARVRPIIERELNFYSKLIEVFVDEANLNIAHTHMPGVAAEGTRMRDDLRGVK
                     SIFDSIKLP"
     gene            complement(835..1737)
                     /locus_tag="VGR03_09770"
     CDS             complement(835..1737)
                     /locus_tag="VGR03_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994607.1"
                     /translation="MPVQEVIIQTRQALQAAVVTNYNLVEAHPPLQHFQLASDIWITE
                     LDYHLAEEVMDCYEPRVFGMPKPVRQSPQLYAFVRLNSAEGSLWDWHPDQQLEQCVAL
                     SRLVHPTSIPLSLHAKISVDENAKLLELIPIETALHGAWIADPDGRDWLTEDDARQLR
                     ELVARLPLSVPKRVSRALWYFEMAARSYFADIRWTLVATGLEALVHTHKERSTYQFKT
                     RTSRLADFLKMTKFTSDAALSFYDLRSQLSHGQGLGTLAVAQQELYREGEDVLRAILL
                     RAILDASFASTFVDNASIVLNWAT"
     gene            complement(1879..2337)
                     /locus_tag="VGR03_09775"
     CDS             complement(1879..2337)
                     /locus_tag="VGR03_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994608.1"
                     /translation="MEKMGMRSFYYSRALREAVFFLWERQKGSKLHCAEFRSKAAAGL
                     RFGKGRLVYDHAIPFNYLQNELLHLPEGFPTDLLRDTLNKFCRVALITRQENDRLQAA
                     AYQSKIPGDWDGIDHLARYKAVGGIEIEKNTFARARQRLDQSVRAGNRSE"
     gene            2976..3845
                     /locus_tag="VGR03_09780"
     CDS             2976..3845
                     /locus_tag="VGR03_09780"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1994609.1"
                     /translation="MSKLTAALRPIYRLLPPKAKALSRFAYFRVKSLRDAPLKARLFG
                     DKADLVAPLYLLEDGPLDYTKLKQNVVNAFHLFLSYGLKPADRVLDIGCGTGRKTLPL
                     LDFLTSGSYEGIDPIEKQVRWCSEKITPRNAKFRFQRVDLRSKFYNPNGKIRPSEYVF
                     PFTDGEFDFVILSSVFTHMFSTEMVRYISEVSRLLKPGGGGLVTFFLLNRESEGLIAS
                     QKSSLNLVYEAEHGSKADNPDRLETAVGHQEAFVMESFHHHGITMQVTEYGSWCGRAN
                     EVYSQDFARLTRS"
     gene            4791..5498
                     /locus_tag="VGR03_09785"
     CDS             4791..5498
                     /locus_tag="VGR03_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994610.1"
                     /translation="MTSDKQASEPHGEGEGRRRGGRFPVLVSVAVRWEETGGKAMKAE
                     AHAREVNIFGGLLQFLDTEVYPAAGVEMELTNLFSGEEARARTAAVRRTKGGAMLGVA
                     VELLIPSETFWGLTFRLRKTTAELVRLDQAIKSGNIDPRVLREFRDAVDYVRKTAWAV
                     QEWQERQAQHHDTATVLPLLVTERVRRATQLCAAVAKDLKESEITSQTAGIEKLFRAS
                     EQLSRDLAELHLPGPDN"
     gene            complement(5655..6485)
                     /locus_tag="VGR03_09790"
     CDS             complement(5655..6485)
                     /locus_tag="VGR03_09790"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1994611.1"
                     /translation="MSFKGKKALVTGSSRGIGRGIALKLAEEGAQIAIHYYRNQAAAQ
                     ATLEKIRGLGSNGFLVQADVCQPDEVRRIFQQVRSEFGSLDIFVSNARTEASTFYEAP
                     MEITLDKWDTAVDSQAKAFLVAVREAVPLMSNGGRIVAITFAPGGRFGSWQPWVAMGA
                     AKAALEVLVRYFAVALARQGITVNTISPGWVEDSVLNSLPEAFQTGLREWQERGWTPM
                     GRLGTPADIGNAVALICSEEAAWITGQLIEVDGGASLVDAHLPLEFQGIAKAQTADAA
                     "
     gene            complement(6641..9331)
                     /gene="acnA"
                     /locus_tag="VGR03_09795"
     CDS             complement(6641..9331)
                     /gene="acnA"
                     /locus_tag="VGR03_09795"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEV1994612.1"
                     /translation="MPLAGNSFGTRDKLNVGARSFQIHRLERLENKGIANIARLPFSL
                     RILLENVLRIEDGRFVRPDDILALAGWKPGVPQKEIAFMPARVLLQDFTGVPAVVDLA
                     TMREAVVKMGGAAGRINPLFPAELVIDHSVQVDVSGNVNAFGLNAQLEFERNVERYAF
                     LRWGQKAFQNFKVVPPDTGICHQVNLEFLARVVCEVSFGSKTEAYPDSLVGTDSHTTM
                     VNSLGVFGWGVGGIEAEAAMLGQPFSMLIPEVVGFKLRGRLSEGATATDLVLTVTEIL
                     RKKGVVGKFVEFYGSGLSSLSVPDRATIANMAPEYGATMGFFPVDAETLAYLRFTARS
                     DEQVALVEAYCKEQGLFRTDETPDPAFSDTLELDLSRVEPTLAGPKRPQDRVGLNEAK
                     ASFLKTVEGAPEKRVQIRNNGDRFELSSGAVVIAAITSCTNTSNPALMLGAGLLAKKA
                     VERGLHTKPWVKASLAPGSKVVTDYLLAAGLTPYLDQLKFNLVGYGCTTCIGNSGPLS
                     EAIGSAIKENDMVAVAVLSGNRNFEGRIHPLVRANYLASPPLVVAYALAGRMDVDLTT
                     EPLGRDLAGKPVYLREIWPSPQETEAAVRAAVSTEMFRKVYGEVFQGDARWNSMPIPE
                     GDLYQWDPKSTYIKMPPYFENMPKDPAPLVDIRGARVLVMLGDSVTTDHISPAGSIPV
                     DSPAGQYLIANGVKPHAFNSFGARRGNHEVMVRGTFGNIRLRNQLAPGTEGGWTLHLP
                     AGEKSAASRSPEKMSIYDAAVRYREEGVPLVVLAGKEYGSGSSRDWAAKGTLLLGVRA
                     VIAESYERIHRSNLVGMGVLPLEFAPGENRASLGLTGHELFSIEGVAALAPRKILRVR
                     ARSADGKETAFSVTARADTPEEVSYYRHGGILQYVLRQLL"
     gene            9417..10793
                     /locus_tag="VGR03_09800"
     CDS             9417..10793
                     /locus_tag="VGR03_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_223146968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HEV1994613.1"
                     /translation="MKIVLISTYELGRQPFGLASPGAWLGQRGHKVECIDLSRQPFDD
                     SVIRSAGLVAFYVPMHTAARLAIPLLEPLRRMNPDAHICFYGLYAPMNEAHLRAMGAE
                     TILGGEFEEGLVHLAGRLAANGTPGPRRMKAQEEPVIHLARQKFILPERRGLPPLREY
                     AHLNLPNGEHRIAGYTEASRGCKHLCRHCPIVPVYKGVFRIVDREVVLADIRQQAAAG
                     AQHITFGDPDFFNGPGHAMAIVKELHREFPRLTYDVTIKIEHLLKHEEHLAALRDTGC
                     LFVTSAVESIDDDVLERLDKGHTRADFLEVVRAFRRLELTFQPTFVPFTPWTRLEDYM
                     EMLDVLLEQDLVGNVAPIQLAIRLLIPAGSRLLELDEVQRIAGPYDPQALVHPWKHSD
                     RRVDALCEEVQQIAATCEKLKRPRHAAFERIFRAAQKAAGREEEAASPPAFPVRAAIP
                     HLSEPWYC"
     gene            10882..12207
                     /locus_tag="VGR03_09805"
     CDS             10882..12207
                     /locus_tag="VGR03_09805"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1994614.1"
                     /translation="MPEGSNSPKKRLLLFAAKLGYQTRSFGEAAERLGVELRFVTDRC
                     HQLEDPWGDHAIPVHFEEPEAAAAAVIEETRGQDVDGIVALGDRPTLAAAYAARRLGV
                     RHNHPAAVEACRNKQRMREVLRGAGLRVPWFRALPVDPIPEPELVGISFPCVLKPLSL
                     SASTGVMRANTHEEFFQAAERIRRLLRSPEVRATREPNLDRVLVEGYIPGREVAVEGL
                     LTDGAMRVLAIFDKPDPLEGPYFEETIYVTPSGLAESQQRAIEQCAREAARAIGLTHG
                     PVHAEFRINEEGIWPLEIAPRPIGGLCSRALRFQADETSEPIFLEELILRHAAGLPGA
                     DWPRERAASGVMMIPVPESGVLEKVEGEEAARATPGITELQITARLHDYIAAWPEGSS
                     YLGFLFARGNSASQVETALREAHAKLRFTLAARLPVEHPAARSATTREA"
     gene            12242..12634
                     /locus_tag="VGR03_09810"
     CDS             12242..12634
                     /locus_tag="VGR03_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994615.1"
                     /translation="MSRRLLVLVLLSGAVIGSATLDGKTRLASQERCAQEHRAWVVQA
                     LQKMQTIQPGMTRKDLLEVFTTEGGLSTGLQRTYVSRECPYFKVDVKFEAVGRPNRDE
                     EGRVTLVEDNRDVIVKVSRPYLQFSTMD"
     gene            complement(12669..13001)
                     /locus_tag="VGR03_09815"
     CDS             complement(12669..13001)
                     /locus_tag="VGR03_09815"
                     /inference="COORDINATES: protein motif:HMM:NF003817.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEV1994616.1"
                     /translation="MANDSCGHAPAAPETVTPGTRMVKCVKFGKELPGLDRIPWKGEI
                     GKRVYENVSKDAWKLWIEHSKMVMNEYRLNPLDPNSQKIMEEQLEQFFFGEGATLPEG
                     YVPPKAKS"
     gene            13149..13769
                     /locus_tag="VGR03_09820"
     CDS             13149..13769
                     /locus_tag="VGR03_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEV1994617.1"
                     /translation="MADITTLFWDIGGVILTNGWDTSSRKEAAEVFQLDWDEFQGRHE
                     LVFPDFDCGRVTLDEYLDRTVFYRPRAFKREEFTAFLFAQSKEYPESRGILDALAATG
                     KYFIAAINNEPLELNQYRIEAFHLKRDFSIFFSSCYVGARKPDPAIYRLALQVTQRPP
                     EQCVFVDDRPLNLEQPRQMGLNVIHYQNPEQLHAELARLGVGTPLR"
     gene            13808..15334
                     /gene="zwf"
                     /locus_tag="VGR03_09825"
     CDS             13808..15334
                     /gene="zwf"
                     /locus_tag="VGR03_09825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV1994618.1"
                     /translation="MSTATSKPQGGFSAQAADPCAVVIFGASGDLARRKVIPALYDLA
                     ARDALAPRYALVGFARTPMTDEAFRTTATEAAKSISEVGPIDAQKWDAFASNLCYSPG
                     EYDNPESYAGLAKRLAALDQEKNLGGNRLFYLSTPPEVYAEIVQQLGRAGLARPSSLK
                     SWVRIVIEKPFGRDLASAHDLNQIVLSVFEENQVYRIDHYLGKDTVQNLLVLRFGNGI
                     FEPLWNRKYVENVQITAAETLGVERRGGFYETAGALRDMIQSHVLQLTCLTAIEPLAS
                     FDANSVRNEKLKVLQSIRPFNLEMVAQSVVRGQYAAGQMGEKAVPAYRDEPGVNATSR
                     TETFVAAQILIDNWRWAGVPFYLRTGKRLAKRTTEIVIQFRCAPHIIFRSRGIEPNRL
                     ILNIQPDEGISISFCAKRPGTEMRIGEVTMKFNYQEAFGEAARSAYATLLNDCLRGDA
                     TLFDRGDTVEAAWRLVDPILEVWSATKSVTPPAYAAGSWGPRESELLLERNGHTWHTP
                     "
     gene            15371..16354
                     /gene="glk"
                     /locus_tag="VGR03_09830"
     CDS             15371..16354
                     /gene="glk"
                     /locus_tag="VGR03_09830"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEV1994619.1"
                     /translation="MILAGDVGGTKCNLALFAAKNGKLTPVAQQRFPSKDYAQFDVLI
                     AEFLQQSASQRGNEPIVSAGFGVAGPVVRNRVKATNLPWIVDGPALAQKLGVAQVVLL
                     NDLGATAHSVGHLSPEDFFVLNPGAAEPGGPKAILAAGTGLGEAVLYWDGARYRVAAS
                     EGGHCDFAPNTEQQIELLRFMRKRSAHVSWELILSGRGFRTLHEFLGPSVRHPSFDDP
                     AGDPAPEITRRGLEGSCAVCVATLDLWCAIYGSEAGNLALRTLALGGVYIAGGIAAKI
                     IEKVKDGTFLKYFFDKEAFEGLLRTIPVLVVLNEAAPLLGAAYQALEAVRK"
     gene            16351..17172
                     /locus_tag="VGR03_09835"
     CDS             16351..17172
                     /locus_tag="VGR03_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994620.1"
                     /translation="MKDARGGRFLNPTGSRGHPMPGMSGAPMVVRIQTAAHFLLILVF
                     YSLLDGAVLPPRLAAQSSPLAAGSSQAAPADDSIPLGDHTGAKRVPARSGDICIQCGH
                     AIGMQDPVYVVNGHRMPLHQRELEGDLPGQLRRLAAQFEPTGAFLQAAARQTGLSPFW
                     FLFGCYVLLGLVCAALCAHRALHKGLPAWVWFLAGLFFNVAAFLAVLLRRGRASVSPG
                     GVPPGLHKISSTHAPVACAKCGDWNHPSARRCVACGASLSPALESEVARSGLRPA"
     gene            17221..19530
                     /locus_tag="VGR03_09840"
     CDS             17221..19530
                     /locus_tag="VGR03_09840"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEV1994621.1"
                     /translation="MLRKLQQQINSVLSSGDMPARRPVLRANNESNISGLYIIGDLAG
                     APVIKLAMAQGFEVIEHIASKPDARGPDPSVYDVLVVGAGAAGLNAALAAQEKGLRTM
                     VLEKGKIANTIEDFPEMKWVYAEPDSRPPKGKLWLDGARKEDLIARWHQIVKENRLEV
                     RMEEELKSLERQKGGGFVLRSSKAEYRARRIVLATGQRGNPRKLGVSGEEQERVYHRL
                     YSPRHYKNEEILVVGGGNSAIEAALVLAEQNRVHLCHRAADFPLAFKDNKRKLDEAVA
                     QKRIELHLNSQAKEFREREVILETVQGGQKEQRPLPMDHAFVLVGAELPVKFLKSLGI
                     KLENEWEGSLWRAAGLAFATLLGLWFLGGHASAVAGVAFERVPGLAGALLAALSFGGL
                     LRLGWRGDRFAWLAVSFLAAYSVYAIKSPGHGSEFWPYRNWGYKFSSFSGRPASFWYT
                     VLYTALMTVFGIQALKRWGLDRKDKFQIWRYASLLSFQWIFFFLIPEFLFQWAVKYQW
                     VGAQLANDPRFAGQAWRSYGIVYSWPLFFYSFFYDPHKIWVIWGIFLTFVLIPVFVIF
                     HGKRYCSWICGCGGLAETFGDRWRHLAPKGDTSIRLEWMNKAVLLFAAVVTVAMLLRD
                     TLVFLRHPATWGVETYHLYVDIWLVGILPVTLYPFMGGKVWCRYWCPLAKLMHVFSAL
                     YAKLGVSRFAITANDKCIGCMECSRNCQVGIPVMNFALKQEPITNANSSCIGCGICVT
                     VCPMDVLDFRGKAPQHVLVQIASSPAHMA"
     gene            19722..20987
                     /locus_tag="VGR03_09845"
     CDS             19722..20987
                     /locus_tag="VGR03_09845"
                     /inference="COORDINATES: protein motif:HMM:NF008589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HEV1994622.1"
                     /translation="MDSLKAMESPQPSGQLAQPGGAGRPGKRSRRWVWLLLLVIVGAG
                     VWYFRSVRAANSVQNAAAAKGGGGRGGNPVVPVVVATAEKGDLPVYLNGLGNVSAFNT
                     VIVRSRVDGQIISVAFKEGQFVQRGSLLVQIDPRPFEVQLAQAQGQLAKDQAQLHDAM
                     ANAARFDMLFKEGVIPQQQRDTQQAAVGQFEGAIKSDQAQIASAMLQLTYSRITAPIS
                     GRVGFRLVDPGNIIHASDAQGLLLINQVQPVAVLFSLPEDQLAQVNSKLRAGVQLSAE
                     AYDRDNTTKIATGKLLTIDNQIDPTTGTYKLKAVFDNSDNALFPNQFVNVHLLVETKK
                     NLTIVPAAALQRGPQGIYVYVVGKDSIARIQSVTLAQTAGNNAGLSAGISPGDVVVID
                     GQDKLQDNTKIDAHTSAGGGAPRPGGQGK"
BASE COUNT         4122 a         6147 c         6687 g         4162 t
ORIGIN      
        1 aatcccaggt gcgggaacgg cacctggggc acccggcgag cagcggtaaa gacaagtcgc
       61 ccacgatgga agatatggtg cacaaagtaa gggaattact gggccggtga aagattcacc
      121 gtggtctttg tagctgggtg atagaatcgt tgaaacggtc gcgcgctggg ggtgggtgga
      181 tggcaataca taagtggaag tttcattttg cggcttgccc ggttttgcta tccgtcatgt
      241 tggcctcagt cctttttagt agcgataata ttgttcgcgg gcaaaccggg ccgcagtcct
      301 ttgacgaagc tgtccatgcg aaacataaac gcttcgtcga gtaccaccaa accttcaaca
      361 gctttgcgca gtccggttta ggttccgatg agttccatgt cgcaatgtcc ctaacgacaa
      421 ttgcctctag aacgggtgac tatctgtggt ccgtgcatat tctcttggag atttacggcg
      481 gcttgtcgtg cgaggaagat agggccagag tccggccgat aatcgagagg gagcttaact
      541 tctacagtaa actgattgag gttttcgtag acgaagctaa ccttaacatc gctcacacgc
      601 acatgcctgg tgtggccgct gagggtaccc gtatgcgaga cgacctgcgg ggagtcaaaa
      661 gcattttcga ttcaatcaag cttccgtagg atgagtgcag cccgggagat ccgagattcg
      721 agcttcgcga ctggtgcttt taagtggacc tcctcaacca cgtggatgtt gcgggtgccg
      781 cacgcttgtg gttttcaagg gtagcagcga gcttcgcttt ccaagacagt tgcatcacgt
      841 tgcccagttg agaacgatgg atgcattgtc gacaaaagta gaagcgaaag aggcgtctaa
      901 aatggcccgt aacagaatgg cgcgtaagac atcctctccc tccctgtaca actcctgctg
      961 cgccacggct agtgttccca gcccttggcc gtggctgagc tgagagcgta agtcgtaaaa
     1021 gctaagcgcc gcgtcgctgg tgaatttcgt cattttcaag aaatccgcta acctggaggt
     1081 ccgagttttg aattgataag tagaccgctc tttgtgcgta tggacgagag cttcgagccc
     1141 ggtggccacc agtgtccagc ggatgtcggc aaaataactt cgtgctgcca tctcgaaata
     1201 ccaaagtgct cgtgaaaccc tctttggaac tgagagcggc aggcgggcca cgagttctcg
     1261 gagttgtcga gcatcgtctt cggtcaacca gtcacggcca tctgggtcag caatccaagc
     1321 gccgtgcagt gcagtttcga tgggaatcaa ttccaagagt tttgcatttt cgtcgacgct
     1381 gattttcgcg tgcagcgaaa gcggtatcga cgttgggtgt acgaggcgcg ataacgctac
     1441 gcactgctct agttgctgat ctggatgcca atcccagagg ctgccctcgg cggaattcag
     1501 ccggacaaat gcatagagtt gaggtgactg tcgtaccggt ttgggcatcc cgaacacacg
     1561 tggctcgtag cagtccatga cttcttctgc gagatgatag tcgagctcgg taatccagat
     1621 gtcgctcgcg agctgaaaat gctgtagcgg cggatgtgct tccactaagt tatagtttgt
     1681 aacgaccgcc gcctgtagag cttgtcttgt ttgaatgatc acttcttgaa cgggcatgga
     1741 acctcgcaga aaacaaattt tgccgttccc cttggagcgg agagcaagct aatcctcctg
     1801 gtccacgtcg gcgaacgatc acgaacgtcg catgatacgc taagcacaac gggtggcgcg
     1861 cccatggcgc tgcggattct attcgctacg attcccagcc ctcaccgact ggtctaacct
     1921 ctggcgcgcc cttgcgaatg tgttcttctc aatctcgatg ccgccaacag ctttgtagcg
     1981 agcaaggtga tcaattccgt cccaatctcc aggtatcttg ctttgataag ccgcagcttg
     2041 taagcggtca ttttcttgcc tcgtgatgag agcgacgcga cagaacttat tcaacgtgtc
     2101 tctgagtaaa tccgttggga acccttccgg aaggtggagc aattcatttt gaagatagtt
     2161 gaaaggaatt gcatggtcgt aaactagccg gcctttgccg aaacggagtc cagctgcagc
     2221 tttcgaccgg aactccgcac aatggagctt gcttcctttc tggcgttccc atagaaagaa
     2281 gacggcctcc ctcaaagccc tggagtagta aaacgaccgc atgcccatct tttccattcg
     2341 ttgacaatgc tctacgactt ctctgatcaa ccctagacgt ttagtttcat ccatgacgtg
     2401 cagtcattct aggcgaatac gcttggcgaa caaagaagcg gactcctgtc ataacgtggc
     2461 cgggtgaggg tcacgggagg agcgagacgg ggcaggcgta aacgacaaag cgaagagaga
     2521 tttctcctcg caaaagacgc tcgaaggagc agaggttctc gctcactcgg aatgacgtgg
     2581 tcggtgggcg gatcgggtgg ggggatttgt tggggctgac gtggtcggtg ggcggagcgg
     2641 gcgggggaga ggatcggtga gggtgctgta caaaaattgt tctcatcggt ccgtggtgag
     2701 gcgtgagtgt tgccaggatc cttccgctgc gcagccagca ctcgcagaag cgagtgaagg
     2761 caggagctgg ccgctccggt caggatgaca acttatggcg agcgaaggcg ggcacggcag
     2821 cgccatggca ctacacggag aagggcggga ggacgaagaa acgtccccac cctcccaaac
     2881 cgagggtggg gcacccgcag ccgctcgtag aagtgcgatc aaaagtgtgg caagctcaag
     2941 cgcgaccgca ccgcgccggg cggattcaga attccatgtc aaagctcacg gcagcactta
     3001 gaccgatcta ccggttgctt ccgccgaaag caaaagctct ttccaggttc gcttatttcc
     3061 gagtgaagtc tctccgcgat gcgcccctca aagcaagact cttcggggac aaggctgact
     3121 tggtcgcgcc gctttatctc ctggaggatg gcccccttga ttacacaaag ctgaagcaga
     3181 atgttgtaaa tgcattccat ctgttcctgt cctatggctt aaagccggcc gaccgggttc
     3241 tggacatcgg ctgcggaacc ggccgcaaga ctttgccgct cctggatttc ctgaccagcg
     3301 ggtcctatga gggaattgat cctatcgaga agcaggtccg ttggtgctcg gagaaaatca
     3361 cgcctcgcaa tgcgaaattc agatttcagc gcgtggatct ccgaagcaaa ttctataatc
     3421 ccaacggcaa aatcaggccg tccgagtatg tgttcccgtt caccgacgga gagtttgatt
     3481 tcgtgattct aagctcggtc tttacgcaca tgttcagcac ggaaatggtg cgttatatat
     3541 cggaggtatc gcgattgctg aagccagggg gcggagggtt ggtgaccttc ttcctactga
     3601 accgtgaatc tgaggggctc atcgcgagcc aaaaatcctc tctcaacttg gtctacgaag
     3661 ccgagcacgg ttccaaggcg gataatccag accggctgga gacagcagtg ggccaccagg
     3721 aagcgtttgt gatggagtcc ttccatcatc acggcatcac catgcaagtt accgaatatg
     3781 gttcgtggtg cggaagagcg aacgaagttt atagccagga ttttgcccgg ctgactcgct
     3841 cttagcccgc cgcgaagcaa gcgaaatccc caccctcgca aaccgagagt gaggcacccg
     3901 cacggagagg cgaagatacc ggtttaaaaa ccggccacta catgaagggc ggggaggacg
     3961 gctctcgcgg ccataaatgg ccgctcttcc accccggatg gtcgctgttc caccccaatg
     4021 gccgctcttc cagcccgaat ggccgctctt ccacccaaga ggccgctgtt ccacccaaga
     4081 ggccgcaata atcgtatgct gcgcgcggca agagcaagaa gggcggactt gaagtccgcc
     4141 cctacattaa gaggggagat gtggtggggg gatcggggag aggtggggcc gcggggttgg
     4201 cgcgcgacgc tcgggatttt ctttgctaca tttcccagcc cttccgggct gggctaagtt
     4261 ctgtcgcgcc cgccagacta cggcgggtct tgcgacctcc ggcgctgttt gacgggaaga
     4321 gggcggcgtg gatgcatccc atcggtccgg gagcggtgga aatcaagcca cgatttcatc
     4381 ccgccaggag cgggatgcgg cagaggtctc gactgcgcgg gcggacaccc cgcgaagcgc
     4441 agggtgaaga aaacgcgtcg gcccgcttcg ctccccgctg acccatccgg ggcaggcggg
     4501 atgacaacgt tggggagggg gcggtgggcc cattcgactc gtccgccacg gcggactcgc
     4561 tcagggtgac ggtggttcga caagctcacc acaagtttct ttatggaatc gtcagagcgg
     4621 ggacggcaca tggggcaccc ggcacacaca caggatcgcg tgctatcatc caagcacggg
     4681 cctacgctag tggcatcctg aaaacaagtt ttgacacttg atccgcatcc actcactgat
     4741 gggttggcag ttgggctcga tttggagagt tttccacctg gagggttccc atgacctctg
     4801 acaaacaggc ttctgagccg cacggcgagg gggaggggcg ccgaaggggc gggcgctttc
     4861 ccgtgctcgt ttccgtagca gtccggtggg aagagacggg cggcaaagcc atgaaagcgg
     4921 aagcgcatgc cagggaggtc aacattttcg gcggactgct ccagttcctg gacacggagg
     4981 tgtatccagc cgccggcgtc gaaatggaat tgaccaattt gttttccggc gaggaggccc
     5041 gggcgcgcac cgcggcggtc cgccgaacca aaggcggcgc gatgcttgga gttgccgtcg
     5101 aactgctcat ccccagtgaa actttctggg gcttgacatt ccggctgagg aagacgactg
     5161 cggaattagt gagactggac caagcgatca agtccggaaa tattgacccg cgcgttttac
     5221 gcgagttccg cgacgctgtg gactacgttc gcaaaaccgc gtgggctgtt caggaatggc
     5281 aggagcgtca agcgcagcac cacgacaccg ccacagtcct tccgttactc gtcacggagc
     5341 gcgttcgccg cgccactcaa ctgtgcgccg cagttgccaa agatcttaag gaaagcgaaa
     5401 tcacttccca aacggcaggg atcgagaaat tgttccgcgc ctcagagcaa ctttcccggg
     5461 acttggctga actgcatctt ccgggcccgg acaattaaaa cgcagctgtc tccttcaacg
     5521 gcacctgggg cacccgcctt cgccaaggct ccggcgggca ggcccttcga acttcgccaa
     5581 ggctacggcg ggcaggccct ttgaaaactc agcgcggatg gagccgcagt gagaagagtt
     5641 gaagaggcgc ctctttaggc tgcgtccgcg gtttgcgctt tcgctatgcc ttggaattcc
     5701 aacggcagat gcgcatccac tagcgacgcg ccgccgtcta cttcgatcaa ctgccccgtg
     5761 atccaagccg cctcttctga gcagatcaac gcaaccgcgt tgccgatgtc cgctggtgtt
     5821 cccaatcggc ccatcggagt ccacccgcgt tcctgccact cgcgtaaacc tgtttggaag
     5881 gcttccggca ggctgttcag tacactatct tcaacccacc ccgggctgat cgtgttgact
     5941 gtgatgcctt ggcgggccag cgcaaccgcg aaataccgca ccagaacctc caaagctgct
     6001 ttcgccgcac ccatcgcgac ccacggctgc caacttccaa accggccgcc gggcgcgaag
     6061 gtgatggcga caatccttcc gccattcgac atcagcggaa ccgcctcgcg caccgcaacc
     6121 aggaatgcct tcgcctgcga atccacagcg gtgtcccatt tgtccaaagt gatctccatg
     6181 ggagcctcat agaaggtgga cgcttctgtc cgcgcgttgc tgacgaaaat gtcgagtgag
     6241 ccgaactcgg agcgaacttg ctggaagatg cgacggacct catcaggctg acaaacatcg
     6301 gcctgcacca aaaatccatt cgacccgagg ccacgtatct tctccagcgt cgcctgcgcg
     6361 gccgcttggt tgcgatagta atgaatggca atctgcgctc cctcttcggc cagcttcaag
     6421 gcgatacctc ggccaatgcc acgggaactt ccggtgacca gagctttctt tcctttgaat
     6481 gacatacgta cccccattcc tacagcgcag catgctagcg cccctgcttg ggacggtcaa
     6541 cggttccagc gttcagccgt tgatcgcact cctgcgcctt cgttgaggct tcggcgggca
     6601 ggcggcgcag cgggtgccgc tacacggagg acaagatgcg ctacagcagt tggcggagga
     6661 cgtactggag gatgccgccg tggcgatagt aggagacttc ttcgggggtg tcggcgcggg
     6721 cggtgacgga gaacgcggtt tcttttccgt cggcggagcg ggcgcggaca cgaagaatct
     6781 tcctcggcgc gagcgcggcg acgccttcga tggagaatag ttcgtggccc gtcaagccga
     6841 gcgacgcgcg gttttcgccg ggggcgaatt cgagcgggag gacgcccatg ccgacgaggt
     6901 tgctgcgatg gatgcgttcg tagctttcgg caatgaccgc gcgcacgccg aggagaagcg
     6961 tgcccttggc ggcccagtcg cgcgatgagc cggagccgta ttcctttccg gcgaggacga
     7021 cgagtgggac gccttcctcg cggtatctca cggccgcgtc gtagatggac attttctcgg
     7081 gcgagcgcga agcggccgac ttctcgccgg cgggaagatg cagggtccat ccgccttcgg
     7141 tgccgggcgc gagctgattg cgcaggcgga tgttcccgaa ggtgccgcgc accatcactt
     7201 cgtgattgcc gcggcgcgcg ccgaaggagt tgaaggcgtg cggcttcacg ccgttcgcga
     7261 tgaggtattg gcccgcggga gaatccacgg gaatcgagcc ggcgggagag atgtggtcgg
     7321 tggtgacgct gtcgccgagc atgaccagaa cgcgggcgcc gcgaatgtcc acgagcggcg
     7381 cgggatcctt cggcatgttt tcgaaatacg gcggcatctt gatgtaagtg gacttggggt
     7441 cccactggta gagatcgcct tcggggatag gcatggagtt ccagcgcgcg tcgccctgga
     7501 agacctcgcc gtaaactttt cgaaacattt cggtggaaac ggcggcgcgg accgcggctt
     7561 cggtttcctg cggcgaaggc cagatctccc gcaagtaaac cggcttgccg gcgaggtccc
     7621 ggccgagcgg ctcggtggtg aggtccacat ccatgcggcc ggcgagggcg taggcgacga
     7681 ccaggggcgg cgaggcgagg tagttcgccc ggacgagcgg atgaatgcgg ccttcgaaat
     7741 tgcggttgcc gctgaggaca gcgacggcga ccatatcgtt ttccttgatg gcgctgccga
     7801 tggcttcaga gagagggccg ctgttgccga tgcaggtcgt acagccgtaa ccgaccagat
     7861 tgaatttcag ctggtcgaga tagggcgtca acccggctgc gagcagatag tccgtcacga
     7921 ctttcgaacc gggagccagg ctcgccttca cccagggctt cgtgtggagg ccacgctcga
     7981 cggctttttt cgccagaagg ccggcgccga gcatcagcgc agggttcgag gtgttcgtgc
     8041 agctggtaat cgccgcgatg acgacggcgc cgctcgaaag ctcgaagcgg tcgccattgt
     8101 tccggatctg cacgcgcttt tccggcgcgc cttcgacggt tttcagaaag gaggctttcg
     8161 cttcgttgag gccgacgcga tcttgcgggc gcttggggcc ggcgagcgtc ggctcgacac
     8221 gggagagatc cagttccagg gtgtcgctga aggcagggtc cggagtttca tcggtgcgga
     8281 agagcccctg ctccttgcag taggcttcga ccagagctac ctgctcatcc gaccgggcgg
     8341 tgaagcggag gtaagcgagg gtttccgcat ccacggggaa gaagcccatg gtcgcgccgt
     8401 attcgggcgc catgttcgcg atggtggcgc ggtccggaac gctgaggctc gaaagcccgg
     8461 agccgtaaaa ctcgacaaat tttccgacca cgcccttctt gcgcaaaatc tcggtgacgg
     8521 tgaggacaag atcggtggcc gtcgcgcctt cgctgagccg cccacgcagc ttgaagccca
     8581 ccacttccgg aatcagcatg gagaagggct gaccgagcat ggccgcctcg gcttcgatgc
     8641 cgccgacgcc ccagccgaaa acgccgaggc tgttcaccat cgtcgtgtgg gaatcggtgc
     8701 cgaccagcga atcggggtag gcttcggtct tgctcccgaa agacacctcg cagacgacgc
     8761 gcgcgaggaa ttccaggttg acttgatggc agatgccggt gtccggaggc accaccttga
     8821 agttctggaa ggccttctgt ccccagcgca agaaagcgta gcgttcgacg ttgcgctcaa
     8881 attcaagctg cgcgttcaag ccaaaggcgt tgacgtttcc ggagacgtcc acctgcacgg
     8941 agtggtcaat caccaactcc gccgggaaga ggggattgat gcgcccggca gcgccgccca
     9001 ttttgacgac ggcctcgcgc atcgtggcga ggtcgacgac ggccgggacg cccgtaaagt
     9061 cctgcagcaa aacgcgggcg ggcatgaagg cgatctcttt ttgcggaacc ccgggtttcc
     9121 atccggccag ggcgagaatg tcgtccgggc ggacgaagcg gccgtcttcg atgcgcagca
     9181 cgttttcgag aaggatgcga agcgagaagg gcaggcgggc gatgtttgcg atgcccttgt
     9241 tttcgagccg ttcgaggcgg tggatctgaa acgagcgcgc gccgacattc aacttgtcgc
     9301 gcgttccgaa ggagttccct gcaagcggca tgagcggcct tctttcgagc gagtccttcg
     9361 taccctatga taaccgaaat cgtctcgacg cgagggaggg ctataatcgt agaggcatga
     9421 agatcgtgct tatttcgacg tacgagctgg gacggcagcc gtttggcctg gcgtcgccgg
     9481 gggcgtggct gggtcagcgc ggccacaagg tcgagtgcat tgacctctcg cggcagccgt
     9541 tcgacgattc cgtcatccga agcgcggggc tggtggcttt ctacgtgccg atgcacacgg
     9601 cggcgcggct ggccattccg ttgctcgagc cgctgcgccg gatgaatccg gatgcgcaca
     9661 tctgtttcta cgggctttat gcgcccatga atgaagcgca cctgcgggca atgggcgcgg
     9721 aaacgatcct gggcggagag ttcgaggagg gcttggttca cctggcgggc cgtcttgcgg
     9781 caaatggaac tccagggccg cgccgaatga aagcgcaaga ggaacctgtc attcatcttg
     9841 cgcggcagaa gttcatcctg ccggagcgtc gcggacttcc gccgcttcga gaatacgcgc
     9901 atctcaacct ccccaacggg gagcatcgaa tcgccggcta caccgaggca agccgcggct
     9961 gcaagcatct ctgccggcac tgtccgattg tgccggtgta caaaggcgta ttccgcatcg
    10021 tggaccgcga agtggttctc gccgacattc gccagcaggc cgccgcgggc gcgcagcaca
    10081 tcaccttcgg cgaccccgat ttcttcaacg ggcccggcca cgcgatggcc atcgtcaaag
    10141 agctgcaccg cgagtttccg cggctcacct atgacgtgac catcaagatc gagcacctct
    10201 tgaaacacga agagcatctc gccgcgctgc gcgacacggg ctgcctgttc gtcaccagcg
    10261 cggtggaatc cattgatgac gacgttctcg aaagactcga taaaggccac acgcgcgccg
    10321 attttctgga ggtggtgagg gcttttcggc ggctcgagct gacatttcag ccgacctttg
    10381 tgccgttcac gccgtggacg cgcctggaag actacatgga gatgctggat gttctgctgg
    10441 agcaagacct tgtcggcaac gtggcgccga ttcaactggc gatccggctg ctgatccccg
    10501 ccggctcgcg cttgctggaa ttggacgaag tgcagaggat cgccgggccg tacgatccgc
    10561 aggcgctggt ccacccctgg aaacattccg accggagggt ggatgcactt tgcgaagaag
    10621 tacagcaaat cgcggcgacg tgcgaaaaac tcaagcggcc acggcacgcc gctttcgaac
    10681 gcatctttcg cgcggcgcag aaggcggccg ggcgcgagga ggaagcggct tcgccgccgg
    10741 cgtttccggt tcgggccgcg atcccacatc tcagcgagcc ctggtactgt tgagcggagc
    10801 cgacggggga ccagttggtc cccatcgcaa aagcccggga agccatgcag aaggcggagt
    10861 gctttgtata gggatggacc gatgccggaa ggctcgaact cgccgaaaaa acggctgctg
    10921 ctgtttgcgg caaaactggg ttatcagaca cggagttttg gcgaggcggc ggagcgcctc
    10981 ggcgtcgaat tgagattcgt caccgaccgc tgccaccagc ttgaagatcc ctggggcgat
    11041 cacgccatcc ccgtccactt cgaggagccg gaggctgcgg cggcggcggt aatcgaagag
    11101 acgcgcggtc aagacgtaga tggcattgtt gcgctgggcg accggccgac gctcgcggcg
    11161 gcctacgccg cccggaggct gggtgtccgg cacaatcatc ccgcggcggt cgaagcctgc
    11221 cgcaacaaac agcggatgcg cgaggtcttg cgcggcgctg ggttgcgcgt tccgtggttt
    11281 cgcgcgctgc cggtagatcc cattcccgaa ccggagcttg tgggcatctc ttttccctgt
    11341 gtgctgaagc cgctttcact atcggccagc acgggagtga tgcgtgcgaa tacccatgaa
    11401 gagttcttcc aggcggccga acgcatccgg cgtttgctgc gctctccaga agttcgagcg
    11461 acgcgcgagc cgaatctcga ccgcgtgctc gtggagggct acattccggg aagggaagtg
    11521 gccgtcgagg ggttgctgac ggacggcgcg atgcgcgttc tggcgatatt cgacaagccc
    11581 gatccgctgg aaggcccgta ttttgaagag acgatctacg tgacgccgtc cggcctggcg
    11641 gaatcgcagc agcgagccat cgagcagtgc gcgcgcgagg cggcgcgggc cattggcctc
    11701 acgcacgggc ccgtccacgc cgaatttcga atcaacgagg aaggaatctg gccgctcgaa
    11761 atcgcgccgc gtccaatcgg cgggctttgc tcgcgcgcgt tgcggttcca ggcagacgaa
    11821 acgtccgagc ccattttcct ggaggagctg attctgcgcc acgccgccgg cttgcccgga
    11881 gcagactggc cgcgcgaacg agcggcctcg ggtgtcatga tgattccggt acccgagagc
    11941 ggggttctgg agaaagtcga aggagaagaa gccgcccgcg ccacacccgg catcacggag
    12001 cttcagatca ccgcgcgtct gcacgactac atcgccgcgt ggccggaagg atcgagctac
    12061 ctggggtttc tcttcgcgcg gggcaattca gcgagccaag tcgagacggc gctgcgtgag
    12121 gcgcatgcga agcttcgatt tacgctggct gcgaggctgc cggttgagca tcccgcggca
    12181 cgttccgcga cgacgagaga ggcataggcg agaacagcgc ggttgaacag gagggtaagc
    12241 aatgagtcga cgactactag tgctagttct actttcaggc gctgtcatcg gaagtgcaac
    12301 tctagatggt aagacgcgcc tggcaagtca ggagcggtgc gcgcaagagc atcgagcgtg
    12361 ggtcgtccag gctttgcaga aaatgcaaac gattcagccg ggcatgacgc gaaaggatct
    12421 tctggaagtg ttcacgacag aaggaggcct gtccacggga ttgcagcgca catacgttag
    12481 ccgagagtgt ccctatttta aggttgacgt gaagttcgag gctgtgggtc gaccaaaccg
    12541 cgacgaggaa gggcgagtga ctttggtcga agacaatcgg gacgtcatcg tgaaagtctc
    12601 gaggccctac ttacaattca gcactatgga ttaggcgcga acggcgcaga gcacgattcg
    12661 gggagcgctt aacttttggc tttcggcggg acgtagccct caggcagcgt ggcgccttcg
    12721 ccgaagaaaa attgctccaa ttgctcttcc atgatcttct gcgagttggg gtccagaggg
    12781 ttgagccggt attcgttcat gaccatcttc gagtgctcga tccacagctt ccatgcgtct
    12841 ttcgagacgt tttcatagac gcgcttgccg atttcgccct tccaggggat gcgatccagg
    12901 ccgggcagct cttttccgaa cttcacgcac ttcaccatgc gcgtgccagg cgtcacggtt
    12961 tccggcgccg ccggtgcgtg cccgcatgag tcattggcca tggttgcctc ctgaagggtc
    13021 caagacttga acatactgca agtccatgaa aattcctagc aggttggagc cttctcatgt
    13081 tctcctgcat cagacgagaa ggccagtgcg cggcttaagt cgtccatcgg aaggggaggc
    13141 ccgaaacctt ggcggatatc acgacgctgt tctgggacat cgggggagtg attcttacca
    13201 acggttggga caccagttca cgcaaggaag ccgccgaggt tttccaactc gattgggacg
    13261 agttccaggg ccgccacgag ctggtttttc ccgacttcga ttgcgggcgc gtaacgctgg
    13321 acgaatacct ggacaggact gttttttatc gcccgcgggc gttcaagcgg gaggagttca
    13381 cagcgttcct gttcgcccag tcgaaggagt acccggaatc gcgcggaatc ctggacgccc
    13441 tggcggcgac cgggaaatac ttcatcgcgg cgatcaacaa cgagccgctg gagctgaacc
    13501 agtaccgcat cgaggcgttc catctgaaaa gggatttcag tatattcttc agttcctgtt
    13561 acgtgggcgc gaggaagccg gacccggcga tttaccgcct ggccctccag gtgacacagc
    13621 gcccgccgga acaatgcgtt tttgtggatg accgccctct caacctggag cagccgagac
    13681 agatgggcct gaacgtgatt cactatcaga atcccgagca attgcacgcc gaactggccc
    13741 ggctgggagt cggaacgccg ctccggtgaa agaggaaggg catccgatcg aaaactgagg
    13801 tgcatggatg agcacggcga cgagcaaacc ccaaggcgga ttcagcgccc aagcggcgga
    13861 tccttgcgcc gtggtgattt tcggcgcgtc gggagatctc gcgcgccgca aggtgatccc
    13921 cgcgctctac gatctggcgg cacgcgatgc actcgcgcca cgctacgcgc ttgtcggctt
    13981 cgcccgcact ccgatgaccg atgaagcctt ccgaacgacg gcgacagaag cggcgaagag
    14041 catttccgaa gtcggcccca ttgatgcgca gaagtgggac gcattcgcct ccaacctttg
    14101 ttattccccc ggggaatacg acaatccgga atcgtacgcg ggactggcca agcgcctcgc
    14161 cgcgctcgat caggagaaaa acctgggggg caaccggctc ttctatctct cgacgccgcc
    14221 ggaagtgtat gcggagatcg tgcagcagct cggccgggcc ggcctggctc gcccctcctc
    14281 tctcaaatcc tgggtgcgca tcgtcatcga gaagcccttc gggcgggatc tggcttccgc
    14341 ccatgacttg aatcagattg tcctcagcgt gttcgaggag aaccaggtct accggatcga
    14401 tcattacctg ggcaaagaca cggttcagaa tctgctcgtt ctgcgctttg ggaacggcat
    14461 cttcgagccg ctgtggaacc gaaagtatgt agaaaacgtg cagatcacgg cggcggagac
    14521 gcttggggtg gagcggcgcg gcggatttta cgagaccgcc ggcgcgctgc gtgacatgat
    14581 ccagagccac gtcctgcagc tcacctgcct gacggcgatc gagccgctgg cttccttcga
    14641 cgccaattcg gtgcgcaatg aaaagctgaa agtgctgcag tcgatccgcc ccttcaacct
    14701 ggagatggtc gcgcagtcag tggtccgcgg gcagtatgcc gccggacaga tgggcgagaa
    14761 agcggtcccc gcctaccgcg acgagccggg cgtcaatgca acctcccgca ccgagacgtt
    14821 tgtcgccgcg cagatcctca tcgacaactg gcgctgggcc ggcgtcccct tctacttgcg
    14881 cacgggcaag cgcctggcca agcgcaccac ggagatcgtc atccagttcc gctgcgcacc
    14941 gcatattatt ttccgcagcc gggggatcga gccgaatcgc ctgatcctga atattcagcc
    15001 ggacgagggc atctccatat ctttctgcgc caagcgcccc gggaccgaaa tgcgcatcgg
    15061 cgaggtcacc atgaagttca actatcagga agcgttcggc gaggccgccc gcagcgcgta
    15121 cgcgaccctg ctgaatgatt gcctgcgggg cgacgccacg cttttcgatc gcggcgacac
    15181 cgtcgaagcg gcatggagac tcgtggaccc gattctcgag gtctggagcg ccacgaagag
    15241 cgtgacaccg ccggcctatg cggcgggaag ctggggacca agagagtcgg aattacttct
    15301 cgagcgaaac ggccacacgt ggcacacgcc ttgagaaaca cccgagcggc ggggtgacgc
    15361 aggggggcgc gtgattcttg cgggggacgt cgggggcacg aagtgcaacc tcgcgctgtt
    15421 cgcggcgaaa aatggaaagc tcacgcccgt cgcgcagcag cgcttcccga gcaaggacta
    15481 cgctcagttc gacgtgctca tcgcggaatt tttgcagcag tcggcttcgc agcgcgggaa
    15541 cgaacccatc gtttccgccg gattcggcgt ggcggggccc gtcgtgcgca accgcgtcaa
    15601 ggccaccaat cttccgtgga ttgtggacgg tcccgcgctg gcgcagaaat tgggcgtcgc
    15661 acaggtcgtt ctcctgaacg acctcggcgc gacggctcac agtgtcgggc atctttcccc
    15721 ggaagacttt ttcgttctca atcccggcgc ggcggagccc ggcggcccaa aggcaatcct
    15781 tgcggccggc acgggcctgg gagaggcggt cctttactgg gatggcgcgc gctaccgggt
    15841 cgcggcgagc gaaggaggcc attgcgattt cgctccgaac acggagcagc agattgaatt
    15901 gttgcgattc atgcgtaagc gttccgcgca cgtcagttgg gagttgatcc tgtccggccg
    15961 gggttttcgg acgctgcatg aattcctcgg cccgtcggtg cggcatccct ccttcgacga
    16021 tcctgccggc gatccggccc cggaaatcac ccgacgcggg ctggaaggat cctgcgcggt
    16081 ctgcgttgcc acgcttgacc tctggtgcgc catttacggc tcggaggcgg ggaatcttgc
    16141 tttgcgaacc ctcgcgctgg gcggggtgta cattgccggg ggaattgccg ccaaaataat
    16201 cgaaaaggtc aaagacggaa ctttcctgaa atacttcttc gacaaagaag cgtttgaggg
    16261 actgttgcgg acgatcccgg ttttggttgt gctgaacgaa gcagcgccgc tgctcggggc
    16321 ggcttatcag gcgctggagg ctgtccggaa atgaaggatg cacgcggcgg gcgattcttg
    16381 aatcccaccg gctcgagggg acatccaatg ccgggcatga gtggagctcc catggttgtt
    16441 cggattcaga cggctgccca tttcctgctc attctggtct tctattcgct tctcgacggc
    16501 gccgttctgc cgccgcggct cgcggcgcag agcagcccgc ttgcggccgg gtcatcccag
    16561 gccgctcctg cggatgactc cattccgctc ggggaccaca cgggcgcgaa acgagtgccc
    16621 gcgagatccg gagatatttg cattcagtgt ggtcatgcga tcggtatgca ggatccggtc
    16681 tatgtcgtga acggccatcg catgcccctt catcagaggg agttggaggg cgatcttccg
    16741 gggcaattgc ggaggctcgc ggcgcagttc gagccaacgg gagccttcct ccaggctgcg
    16801 gcgcggcaga cggggctttc gccgttctgg ttccttttcg gttgctatgt gctgctggga
    16861 ctcgtttgcg cagctctctg cgcacatcgg gccttgcaca agggattgcc ggcgtgggtt
    16921 tggtttctcg cgggactctt tttcaacgtg gcggcgttct tggccgtctt gctgaggcgc
    16981 ggccgggcat ccgtctctcc gggcggcgtg ccgccggggt tgcacaagat ttcctccacc
    17041 cacgctccgg tggcctgcgc gaagtgcggc gactggaatc atccctccgc gcggaggtgt
    17101 gtggcctgcg gcgcatcgct gagcccggcg ctcgaatccg aagtcgcgcg gtccgggctg
    17161 cggcccgctt gacccagacc tatttccgga tcgaacagag cggagagaat caagagccca
    17221 atgcttcgga aactccagca gcagatcaac agcgtcctct ccagcggaga catgcctgcc
    17281 cggcgccctg tccttcgcgc gaacaatgaa agcaacattt cgggtttgta catcatcggc
    17341 gatctcgccg gagctccggt catcaagctg gccatggcgc agggatttga ggtcatcgaa
    17401 cacatcgcgt cgaaaccgga cgcacgcggg ccggatccat ccgtgtacga cgtgctggtt
    17461 gtgggcgcgg gcgcggcggg gctgaacgcg gcgctggcgg cgcaagaaaa aggactccgc
    17521 accatggtcc tcgagaaggg aaagattgcc aacaccatcg aggacttccc ggaaatgaag
    17581 tgggtctacg ccgagccgga ttcccggccg ccgaaaggaa agctttggct ggacggcgcc
    17641 cggaaggaag acctgatcgc gcggtggcac cagatcgtca aggagaaccg cctcgaagtg
    17701 cggatggagg aagagctcaa gtccctcgag cggcagaagg gcggcggttt tgtcctccgg
    17761 agcagcaagg cggaatatcg cgcgcgacgc atcgtgctgg ctacggggca gcgcgggaat
    17821 ccgcgcaagc tgggcgtgtc cggcgaggag caggagcgcg tttaccatcg cctgtacagc
    17881 ccgcggcatt acaagaacga agaaatcctg gtcgtggggg gcggcaacag cgccatcgag
    17941 gctgcgctgg tcctcgcgga gcagaatcgc gttcacctct gccaccgcgc agcggatttt
    18001 ccgctggcct tcaaggacaa caagcgcaag ctcgacgaag ccgtggcgca gaagaggatc
    18061 gagctgcacc taaattcgca ggcgaaggaa ttccgcgagc gcgaggtgat cctggaaacg
    18121 gtccagggag ggcagaagga gcagcgtccc cttccgatgg atcacgcgtt cgtactggtc
    18181 ggcgccgaac tgccggtgaa attcctgaaa tccctgggca tcaaactcga aaacgaatgg
    18241 gaaggcagcc tgtggcgggc ggccggattg gcttttgcga cgttgctggg gctctggttc
    18301 ctgggcggtc atgcatcggc cgtggcaggc gtcgcctttg agcgggtccc cggcctggcg
    18361 ggcgccctgc tggccgctct gagttttggc ggactcctcc ggctgggctg gcgcggagat
    18421 cgcttcgcct ggctggcggt ttccttcctc gccgcttact cggtctatgc gatcaagtcg
    18481 cccggccacg gatctgaatt ctggccctac aggaattggg gctacaagtt cagctccttt
    18541 tccggccgcc cggcgagctt ttggtacacc gtgctgtaca ccgcccttat gaccgtgttc
    18601 ggcatccagg cgctgaaacg ctggggcctc gaccgcaagg acaaattcca gatctggcgc
    18661 tacgccagcc tgctcagctt tcagtggatc ttttttttcc tgattcccga gtttctcttt
    18721 cagtgggccg tcaaatacca gtgggtggga gctcagctcg cgaacgatcc acgatttgcg
    18781 ggccaggcgt ggcgcagcta cgggattgtt tattcgtggc ccctcttctt ctattctttc
    18841 ttttacgacc cgcacaagat ttgggtcatc tggggaattt tcctcacctt tgtcttgatt
    18901 ccggtattcg tgattttcca cggcaagcgc tattgctcgt ggatctgcgg ctgcggcggc
    18961 ttggcggaaa ccttcgggga tcgctggcgg catctggctc ccaaaggcga taccagcatc
    19021 cgcctggaat ggatgaataa agccgtactg ctctttgcgg ctgttgtgac cgtggcgatg
    19081 ctcctgcgtg acaccttggt ctttctccgt catcccgcca cgtggggagt cgaaacctat
    19141 cacctgtatg tggacatctg gcttgtgggc attctgcccg tgacgctcta tcccttcatg
    19201 ggcggcaagg tctggtgccg ctactggtgt ccactcgcga agctcatgca cgttttcagc
    19261 gccctctacg cgaagctggg cgtcagccgc ttcgccatca ccgccaatga caagtgcatc
    19321 ggttgcatgg aatgctcgcg gaattgccag gtgggtattc cggtaatgaa tttcgcgctg
    19381 aagcaggaac ccatcaccaa cgcgaactct tcctgtatcg gttgcggaat ctgcgtgacg
    19441 gtctgtccga tggacgtcct cgattttcgc ggcaaggcac cgcaacacgt gcttgtccag
    19501 atcgcttctt ctcccgctca catggcatga gcgttcgccg gaaaaagacg atcgaatgcc
    19561 gtaacccgtc cgttgctcgc ggcgtccaat aacagcgtga gtcaagcggg ccgcaacggc
    19621 gtcaaactgg cgtttcagcc cgcaggtatg tttgaatctt tgattcctcg ttatcccgtg
    19681 ctgtcgtggt tgtttcggtc cctctcgaga ggatgctgag gatggacagt cttaaagcga
    19741 tggagtcgcc gcagccgagc ggacagttgg cgcaacctgg gggagcgggc cgtcccggca
    19801 agaggagtcg caggtgggtc tggctgcttc tcctcgtgat cgtgggcgca ggtgtttggt
    19861 atttccgcag cgtccgtgca gcgaactccg tgcaaaacgc ggcggcggca aaagggggag
    19921 gcggccgagg cggcaatccc gtcgtgcccg tggtagtagc gaccgcggaa aaaggcgatc
    19981 tgcccgttta tctgaatggt ctgggcaatg tatctgcgtt caacacggtc atcgtgcgca
    20041 gccgcgtgga cggacagatc atcagcgtgg ctttcaagga ggggcagttc gtccagcggg
    20101 gcagcctgct ggttcagatc gatccgcgcc ccttcgaagt gcagctcgcg caggcgcagg
    20161 gccagttggc caaggatcag gcgcagctcc atgacgcgat ggccaacgcc gctcgcttcg
    20221 atatgctttt caaggaaggg gtcatccccc agcagcagcg cgacacgcaa caggcagccg
    20281 tcgggcagtt tgagggagcg atcaagtccg atcaagctca aatcgccagt gccatgctcc
    20341 agctgaccta ttcccgcatc acggctccga tcagcgggcg cgtcgggttt cgcctcgtcg
    20401 atcccggcaa catcattcat gcgagtgatg cgcagggtct tctgctcatc aaccaggttc
    20461 agcccgtcgc cgtcctcttc agcctgccgg aggatcagct ggcccaggtg aacagcaagc
    20521 tccgcgccgg cgtgcagttg tccgccgagg cctacgaccg cgacaacacc acgaagattg
    20581 ccacgggcaa gctgctcacg attgacaatc agatcgaccc caccaccgga acttacaagc
    20641 tcaaggccgt cttcgacaac agcgacaatg ctttgttccc gaatcagttc gtcaatgtcc
    20701 acctgctcgt cgagacgaag aagaacctga cgattgttcc cgcggcggcc ctgcagcgcg
    20761 ggccgcaggg aatctatgtc tatgtcgtcg gcaaggacag catagccagg attcagagcg
    20821 tcactctcgc gcagacggcg ggcaacaatg cgggcctcag tgcggggatt agtcccggcg
    20881 atgtggtggt gattgacggg caggacaagc tccaggacaa cacgaagatc gatgcgcaca
    20941 cttccgccgg agggggagcg ccgcgcccgg gaggacaggg gaaatgagtc cgtcccggcc
    21001 gttcattctg cggccggtcg ccaccaccct gctgatggtc ggggtgctgc tcgccggctt
    21061 tgtcgcctac cggcagttgc ccgtctcggc cctgccgcag gtggactatc ccacgatc
//
LOCUS       DASYVO010000298        15429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_52491, whole genome shotgun sequence.
ACCESSION   DASYVO010000298 DASYVO010000000
VERSION     DASYVO010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 15429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15429
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52491"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..748)
                     /locus_tag="VGR03_09850"
     CDS             complement(227..748)
                     /locus_tag="VGR03_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994623.1"
                     /translation="MVLTGNLFVLVLRVLKVKEVILERPNIALSQRPAWRKLAAEVLG
                     TGLAPTPGVLGNPAEDQEWKDWYDALQEFLLKGWPVLSSEVQLLWAHLQATGWALLAL
                     YFKTTIRGHWSVVLVAVSLILTSIGLPVMSMFTYWTMDRLTYWDFMGRLLHELRTREA
                     VKQAQAAQPPQNS"
     gene            complement(1139..1918)
                     /locus_tag="VGR03_09855"
     CDS             complement(1139..1918)
                     /locus_tag="VGR03_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV1994624.1"
                     /translation="MTETLAQRFKAMLWVEPDLNRKYYAITVLGSELAVEWSCISHWE
                     GFTKDDPPREIRAASLVNAKNVAQTWRDIGQPYVVVNSLDDMVVFMLLAGNALIERAV
                     AEETISEFLAAHPVAQVGVFGFRHIESLPKGALNRAPTPRHRMRILKRDDFRCRVCGR
                     RSTDHVDVELHVHHIRPWAQGGVTEDSNLITLCHTCHKGLEPHFEWKLFDLASWGGKA
                     FDLKHKQQQYKEGVAYYRRRMLEEHRRRRGAPGSGDGRNVF"
     gene            2919..3278
                     /locus_tag="VGR03_09860"
     CDS             2919..3278
                     /locus_tag="VGR03_09860"
                     /inference="COORDINATES: protein motif:HMM:NF042549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6632 domain-containing protein"
                     /protein_id="HEV1994625.1"
                     /translation="MGRERVLKVVLVVVGLVFCALVYPLALFWKQAPALAMMLSLYVT
                     LGVFLLLAVRDTSANRSLIAFTAWSSFAHALVMGMQALRHLIQPGELWGVAMLIVIGI
                     VLIALAPGKKPVLTSPS"
     gene            3545..3769
                     /locus_tag="VGR03_09865"
     CDS             3545..3769
                     /locus_tag="VGR03_09865"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEV1994626.1"
                     /translation="MSYADIITMEPGKRGGKPCIRGLRITVYDVLEYLAGGMTHEDIL
                     RDFPYLTEQDIRACLAFAADRERKSEMLSA"
     gene            4277..4576
                     /locus_tag="VGR03_09870"
     CDS             4277..4576
                     /locus_tag="VGR03_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994627.1"
                     /translation="MVRKPIVALFILAVCLVAVSCKKLGEPRPGPTGPLTFETVKFTD
                     AIPKDYGPLIGVTQNPAAPGWAGLWFQRSDGTITAVFVNVDQGQIYQRALTIPRK"
     gene            4618..4845
                     /locus_tag="VGR03_09875"
     CDS             4618..4845
                     /locus_tag="VGR03_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994628.1"
                     /translation="MSLEKLISTMSRLFFFGAFVLLGLALIERIANATGYTILQLYRA
                     GRLLEVAVVLLVFVIAMQLREMKEELKNRKP"
     gene            complement(5007..5222)
                     /locus_tag="VGR03_09880"
     CDS             complement(5007..5222)
                     /locus_tag="VGR03_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994629.1"
                     /translation="MKKEYDFSKLKELKNPYAGKKKPVGINLSPQVVDYFKGLADETG
                     LPYQKLIDLYLLDCAKKRKKLTMKWVA"
     gene            complement(5599..6585)
                     /locus_tag="VGR03_09885"
     CDS             complement(5599..6585)
                     /locus_tag="VGR03_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEV1994630.1"
                     /translation="MKTSFVTDLNSEQSITSYFLVHEKEIRSTREGKPYLRLELGDRS
                     GTIEARMWDQFDAVVKDISRDDFVKVNARVEIYRNKPQLSLQQLRLAKPEEVDLADFL
                     PHTKEDVGKLYAQLLEYAGSLGNPWLKKLVTGIISDPGIAAKYKRAPAAKVMHHAYLG
                     GLLEHVIGLCGLSKQIAAHYPELDVDLLLTAAMLHDVGKLEELCYERAIAYTVEGQLL
                     GHIVMELETVSKAMDAIEGFPANLKTVVQHLLISHHGQYEFGSPKLPMIREAMAFHYM
                     DDLDSKLAAVRAALAVDTGEPEWSAYSGALGRKFLRLDAFLKGPTSASAERK"
     gene            complement(6582..7820)
                     /locus_tag="VGR03_09890"
     CDS             complement(6582..7820)
                     /locus_tag="VGR03_09890"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV1994631.1"
                     /translation="MRNLVLIGALCAALPLAAQDKPQGPQPKPPAGPTLEKPGMAAPD
                     PKSEKPAAKPVAPKPSNPDSGKTVEEIIARVNNEIITRSEYNKARQTSEEDAKSECQN
                     RCTPEQLQTDIEDRQKHTLRDLVDQSLLVQRAKDMGVSVEPELIKKLDTIRIENKLPS
                     MEELEKAVSGQGMNWEDFKNNIRSTLLTQRVISSEVGSHITISDEEVTKYYEAHKAEF
                     VRPEQVALREIVVNTEGKKPEDLPDLKKKAETALKRVQDGEDFGEIAKRLSDGSTKSQ
                     GGFLGVYKRGELSKELEDKVFKMKRKELTGVMETKQGFLVLEVLEHYDEGEQALAKVK
                     NEIMDKLYGGRMEPAMREYLKTLREQSYVVIKPGYQDIAGGGGSEILEVSATPEVSKQ
                     KKSHKKFLLFGKRSGQQSGT"
     gene            complement(7842..11273)
                     /gene="mfd"
                     /locus_tag="VGR03_09895"
     CDS             complement(7842..11273)
                     /gene="mfd"
                     /locus_tag="VGR03_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV1994632.1"
                     /translation="MILPAVRERLEAVLRHAAMEGALAALRSGASHISITGLHDVAKA
                     LVAAYLTRELRRPAFFVTDSNRRAEALAETLRFFSGIFPGAAGGVATLPAFDRLPWES
                     QSPHADILERRAATLFRVADGQISLVIAPIAAALWRYQDPAAYLYLTRTLAKDTEIPH
                     DELIAHLGSVGYTRTEMVELPGQFAVRGGIIDVFSPEAARPVRIELLGDTVESVREFD
                     PRTQRSIAPVQRTTLLPLTEWSVPAPEKANLNDASWETPSFFGPTSEPGPSTLFELAE
                     SSLRPIVFLDEPQTLREAAAKHLAAATENYERHGRANSPAASHYFWSEAELVAALDKS
                     SQIHIEQLALGIGSTPQFELSSRPSARFHGNVVACMGDVKSQLASGGTVFLTAASTGE
                     IERFADICREYEVPYVLGESEDAAAGFTAEGAHESAAMLLIRAPFAEGVTFPGARLTL
                     FGHADLFDVTPTVERPSRKIRTSGFFGDFAELKSGDYVVHVDHGIGQFEGLRQIESGG
                     HSGEFMLLRYADDARLYVPLERMDLVQSYRVLEGTHPPLDKLGGTVWTARKTRARKSV
                     EDMADQLLALYAQRKTTEGFAFSPDGNFQREFEDAFEFEETTDQITAISDIKRDMERA
                     EPMDRLLCGDVGYGKTEVAMRAAFKTVMDNKQVVVLAPTTVLTFQHFETFKKRFAAFP
                     ARIEMLSRFRSAAEQKKILADLEAGKVDVVIGTHRLLSKDVKFADLGLLVVDEEQRFG
                     VAHKERLKEMRKNVDALALSATPIPRTLHMSLVGLRDMSVIETPPRDRLAIQTVVAPF
                     QEELVQSAIETELARDGQVFFIHNRVESIYSLGALVAKLVPKARVVVAHGQMVEKELE
                     SAMLKFIRDEADVLVATTIVENGLDIPRANTILINRADRLGLAELYQLRGRVGRSAQR
                     AYAYLLVPPDTSLSDIARKRLAAMKEFSELGAGFRIAALDLELRGAGNMLGRQQHGHI
                     EAIGFDMYCQMLERAVSKLKGEEVAPDLRTTLSLGFDVRIPQDYIPSENLRLRTYKRV
                     SSIASEADKQDVRKELEDRFGKPPTSVENLLEYAALKSMCERLRISAVERQGTRIAVR
                     FHHETTLDPAKLVSVVRSRTGIKLDPSGVLWMEVKRGESIPAALRNVLLGLQGQG"
     gene            complement(11284..11781)
                     /gene="rfaE2"
                     /locus_tag="VGR03_09900"
     CDS             complement(11284..11781)
                     /gene="rfaE2"
                     /locus_tag="VGR03_09900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEV1994633.1"
                     /translation="MNSAVLTIEEAILRFGREKRNGLRVVFTNGCFDLLHPGHIRSLE
                     LARELGDALIVGLNSDASVRQLKGEGRPVISERERAEILAALESVDAVVIFDDLTPRE
                     VIARLLPDVLVKGGDWPGDQIVGREEVEAAGGRVVSIPVVPGYSTSAILRRIREGARA
                     SRAES"
     gene            11882..13390
                     /locus_tag="VGR03_09905"
     CDS             11882..13390
                     /locus_tag="VGR03_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV1994634.1"
                     /translation="MKSRQAIRAIAGVLLCAVGLWLALPTPYRERTFLFDAGGCRLET
                     TIVEKQEGASQGSVLLFHGISANKKIMSYLARGFAEQGLRVYVPDLPGHGRTPGPFSP
                     ARAEQCGEALLRELLARRLIDADRTILAGHSMGGAIAERIASRVSVAGLIAISPAPMR
                     AVHGVTAEKLLFSDPPTLPPHSLVMVGSLELESMRGNAADLAASRSDGTAKYLEIQGA
                     SHVSVLFNHAAMRASQEWSAETLHLPPTAALPSHRPLIGALAGFLGILLIAGPFLREV
                     CGKNRSEEIAATVAAISMTRLLLEFAVGSVLIVLLLRFWIPLKAIRLFQGDYLASFLL
                     LLGAVLIVTHWSSVRMALTNPSRHLLAAGFAGVVLLLLAKAWFDLTFYEAWLIGAKWV
                     RFPFLLVVLLPYHIAEEALLGPVERGKKWRRLALALSLRLTTWGAMMAGILLLHSGEI
                     LIGLLAFYMALFNVVQRSGMDIVREETGSAAATAVFGAILLAGFCLVIFPLT"
     gene            13483..14673
                     /locus_tag="VGR03_09910"
     CDS             13483..14673
                     /locus_tag="VGR03_09910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1994635.1"
                     /translation="MAQIAIVGGGPSGAMCGEQLARAGHKVNLFDEHLAWEKPCGGGL
                     THKAIQCFPFLLDNSYPKKLVNSVELISSEEHRAILEMPHPIVIYSRTVLNGMLLDRA
                     REAGCNVQRSRVMSVDTTTAKPRYCVEGDWQEADFLVLAAGARNQLLPETRALQRDEL
                     EMTQGYFVPQTSDSITIKFLRNFEGYIWSFPRCDHLSVGICGSMSSHTSTELRGHLQT
                     FVEKHGIQTEGAKFFSHVLPSPKERTLSERNVIGKNWALVGDAAAWVDPLTGEGLFYA
                     IRSGELLGRSLAEGCPEKYPAWVKATFCAELEFAARIVRRFYRGSFLGSAVTTRMVQF
                     LRRSPVFRQLMGDLFSGTQDYTSLKRRLWGHLGITVSEFISSVLSLDRPARTSVPRTG
                     TAGD"
     gene            14764..>15429
                     /locus_tag="VGR03_09915"
     CDS             14764..>15429
                     /locus_tag="VGR03_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1994636.1"
                     /translation="MATETASKSPAKSEKRKNAWKNLPDVWALMKPRRGLLALGFVLM
                     AVNRLSGLVLPASTKYLVDNVIGNRQIQLLTPIVLGVLAATVLQGLTSFTLTQLLSKS
                     AQKMIADMRRQVQAHIGRLPVAFYDANKTGALVSRIMSDVEGVRNLVGTGLVEFVGGL
                     MTAVIALVYLLHTSVTMTAVAFAILLVFGYGINKAFTTIRPIFRARPKINAEVTGRLT
                     ESLS"
BASE COUNT         3067 a         4393 c         4574 g         3395 t
ORIGIN      
        1 tcatggtcgt agtacaaggc cgtatcgatg gctggtggaa cgatttgttt caccgaaaac
       61 tgattcaccg tgagagttcc agagcttcgg tcctcaacca ctctggcagt cacgcggtgc
      121 ttcacagcat tgccacggag aagtgtctct tgacatcgcc ttctctctta tcgaaggcca
      181 ccccgaggcg tttttcgaaa ttaatgtgcg gtcacccgcc tagcgctcaa gaattttggg
      241 gaggctgggc ggcttgcgcc tgtttcacgg cctcgcgcgt gcgaagctcg tgcagcagcc
      301 gacccatgaa atcccagtac gtcaagcgat ccatagtcca gtacgtgaac atcgacatta
      361 ccggcagccc tattgaggtc aaaatcaacg aaactgcgac caaaacaacc gaccagtgcc
      421 ccctgatggt cgttttgaag tacagcgcaa gtaatgccca gccggtggcc tgtaggtgtg
      481 cccaaaggag ctggacttcg cttgagagaa caggccagcc tttgaggaga aactcttgca
      541 gcgcgtcgta ccagtccttc cattcttggt cctctgccgg attgccgagt acgcccggcg
      601 ttggcgcaag gccagtgccg agaacctccg ctgctagctt gcgccatgca ggacgctgcg
      661 aaagcgcgat gttcgggcgc tccagaataa cttccttgac cttgaggacc ctcagcacga
      721 gcacaaacaa gttgcccgtg aggaccaagc taaatccgta taacaacaat ccaacgatgt
      781 aagaggcgaa gatcagagcg cccgtgcgag aatagggccc caagcccaag gcaactgtca
      841 ccttggggag aaaatcctgg tgggccagta gtaagccgcc aacaagcgtt cctcccggaa
      901 caaggggaaa aacacctgat aaatgcggtc aaacgtgacc tgtccgaagt cgacgcgtat
      961 ctcgggtacc attattgagg gagaccttat cacaaataaa gactctgggg gatggcagcc
     1021 cttggcggct ggggggagcg ggacggggag cggggacggg agcggggaca gacgggaagt
     1081 ttcccttacc ctaaaatttc aaatcgttgt aagccgaacg agtcttgtca gacaaatatc
     1141 agaaaacgtt ccgtccgtcc ccgcttcccg gcgctcccct acgtcgacgg tgctcctcta
     1201 acattcgacg ccggtagtaa gcaacgcctt ccttgtactg ttgctgctta tgcttgagat
     1261 caaatgcctt accgccccag gaagccaaat cgaagagctt ccactcgaaa tgaggctcga
     1321 gtcctttgtg gcaggtgtgg caaagcgtaa tcaggttcga gtcctccgta acacccccct
     1381 gggcccatgg gcgtatgtgg tggacatgca gctccacgtc cacatgatct gttgaacgcc
     1441 gaccacaaac ccgacaacgg aagtcgtcgc gcttcagaat gcgcatccga tgtcttggag
     1501 taggggctcg gttaagagca ccctttggaa gggattcgat atgcctaaat ccgaagacgc
     1561 cgacttgggc aactggatga gccgccagga attcggagat cgtttcttcc gccacagctc
     1621 gctcgatcaa agcgtttcct gcaaggagca taaaaacgac catgtcatct agggagttga
     1681 cgactacgta gggttgccct atgtctcgcc acgtttgcgc gacgttcttg gcgttcacaa
     1741 gagaagcggc gcgaatctca cgaggagggt catccttagt gaagccctcc cagtgcgaga
     1801 tgcatgacca ctcgacagcg agctcgctgc caagcaccgt aatcgcatag tatttccggt
     1861 tcagatcggg ctcgacccaa agcatggcct tgaagcgttg cgcaagagtc tcggtcatgc
     1921 tccgccgatc ctctcatact tcacggggcg ggaggtcggt aggccggttt gcgtatagga
     1981 ctttttcccg ggatcatgtc ttttacaaac caaatcagac tcctttggga aattcccgta
     2041 tatcgggctg atcgtattca tattccttgc cgcaattggg gcacttcact cgccctgctt
     2101 ttttaactac gacaaaagag actaagtcgc cgccacgctc tggcctagtc aaatagctgc
     2161 ccaagataat gccagtgtga cattccgcat tcttgcattc gactccgtat gacctttcct
     2221 ctgacatttt gtaccccctc cttactttcg cgtagccaga attgacccac agagcgattg
     2281 cagtaaatcg gacgctgatg ggaccagaaa cgcccggaaa ccaatcaacg cccattcatt
     2341 ctccgtaacc gcgtttcagc agcggaacgt ccgcaactca aacaggaatt ctcggcgtgg
     2401 tcaagcatgt atacacaaac gccgggtgct ccgcacgccg ctcttgcgtg tgggggtttt
     2461 gaaccttcct cagagcgtgc ctttagtcac gacgcggtca ggcggctggc gcatccttaa
     2521 caagctgaga cggctccggg tgggcgggtg gcgcttcctt aacaggctga gacatctccg
     2581 gtcggcctac cctttgcgat tttgttttgt gcgcgtcgtt tgtgtcccgg acgattctgc
     2641 gggacaaagg gtgggcaatt ctttgcttcg ctttaaccag ccgccgacga tgcggctgtg
     2701 cgtccggcga gttgaaacgg tcttcagtga tcagttttca gttttcagta agagaagaga
     2761 aaatccgtag aacaatggat gcccttgatg aacgagtcga gctgtcccag ttccaggtag
     2821 tgggcccgtt tccgataatg cgactaacca ggtgttgcgt gccctccatg tgctataaga
     2881 actgattggg tccccgcgtg atgtagcagg aggagcgtat gggtcgtgaa cgcgtgttga
     2941 aggttgtgtt ggtcgtggtg ggcttggtct tctgcgcctt ggtttatccc ttggcgctgt
     3001 tttggaagca agcacccgcc ctggcgatga tgcttagcct ttatgtcaca ctcggcgtgt
     3061 tcctgctgct ggcagtgcgt gacacatcgg caaatcgcag cctcatcgct tttaccgcat
     3121 ggtcgagctt tgcgcacgcc ttggttatgg ggatgcaggc gttgcgccat ttgatccaac
     3181 cgggagaact gtggggtgtg gctatgctca tcgtcatagg tatcgtcctg atcgcattgg
     3241 ctccgggaaa gaagccggtc ctgacatcgc cgtcttgaat aaagcccacg gcagagtaag
     3301 cgaagctgaa acaacggggt attccccgtc tcttggtttt agtgttgagt gatagtttgc
     3361 gaaatcaggc gggtggcgca tccttaacag gctgagacgg ctcctggtgg cctacgcttt
     3421 acgattttgt tttgtgcaaa gggtgggcaa ttctttgctt cgctttaaac cagccgccga
     3481 cgatgaagcg ggtgggacag gctttccgat tgcccggggg cggtttttca cttaaagtgg
     3541 ggctatgagt tacgccgaca ttattacgat ggagccgggc aagcggggcg gaaagccgtg
     3601 cattcgcggg ctgcgcatca ctgtctatga cgtgctggaa tatctcgcag gcggcatgac
     3661 gcatgaggac atattgcggg attttcccta tttgacagag caagatatcc gtgcctgtct
     3721 ggcgttcgcg gctgaccgcg agcgcaaatc ggaaatgctg agcgcgtgaa gcttcttctg
     3781 gatgaaaatc tttctcctcg gcgggtggcg catccttgcc aggctgagac ggctccgggc
     3841 ggcctaccct ttgcgatttt gttttgcgca aagggtgggc aattctttgc ttcgctttaa
     3901 accagccgcc gacgatgagg ccgtggcgtc cagcgctggc ggcctaccac ccttgatgac
     3961 gagccgaact gccccagttc ccggtcgtgg gccagtttcc gataatccga cttatcagga
     4021 gttagggcgc gtggccgaga ctctgtatca gtcagcagga actgcacatg gattgctccg
     4081 cgtaacacac cagttatgga aagcgtctcg tcagcctgtc gcgacaactt tgcgactccc
     4141 cgcaggtaca accaggtgta caatcccacc agcctgggga ctcaaaacgg gaatccaggg
     4201 taagacttcg cgcaagcgca ggcacggcca taagtgttgg gttcaccttt ggggctccag
     4261 gcgagagagg tgcacaatgg tgagaaaacc tatcgtcgca ctattcatcc tagctgtgtg
     4321 tctggtggcc gtctcttgca agaagctcgg cgagccccgt cccggcccga cgggaccgct
     4381 gaccttcgag acagtcaaat tcactgatgc catcccaaaa gactacggac cgctaattgg
     4441 tgtaacgcag aacccggcag ctcctgggtg ggccgggctt tggttccaga gatcggacgg
     4501 gaccatcact gccgttttcg ttaacgtcga tcaggggcag atctaccaaa gggcgctgac
     4561 gattccacgg aagtagtggg ctcgcagcaa tcacgttaag gaggttgaag gataactatg
     4621 agccttgaga agctaatttc gacgatgtcc cgccttttct tttttggggc gttcgtgttg
     4681 ctcgggctgg ccttgattga gaggatcgcg aacgcaactg gctacacgat cctacaactg
     4741 taccgtgcag gtcgtctgct ggaggtcgcg gtcgtacttc tggtcttcgt gatcgccatg
     4801 cagctgagag agatgaagga agaactcaag aacagaaagc cctagggcgg gtggcgcacc
     4861 ctacggtttt cagggtgcgg tttttcccgg atttcatcat catcactgaa acagctttgg
     4921 gttgggcacg ttttcttccg ctttggcact agtggacctc aagaactgat ccaggcggag
     4981 gaatttgcgg ccgagggccc cgtaggctat gccacccact tcatggtcag cttcttgcgt
     5041 ttcttggcgc agtcaagaag atagagatca atcaattttt ggtaaggaag tcccgtctca
     5101 tccgccagtc ctttgaaata gtccacaact tgcggactca agttgattcc cacgggcttt
     5161 ttcttgccag cgtacggatt cttcagttct ttcaatttgg aaaaatcata ttccttcttc
     5221 ataaaatcga acctccttct tggttgcctt ccgcgcagaa ataatccgaa tcaaggaatc
     5281 ggagtcctta tagcagtgaa cgacgaccag cgctcttgcc cctgaaccta tcccgaggag
     5341 gatgaatcga tcctcctcct cggaatgttc tggatcatag aacaggcgtg catgcggatc
     5401 gtgaaaaaca ctctgggctt cttcaaacca aactccgtgc ttggtacgat tgcccctatt
     5461 tttccgttcg tcccaatcga atcggagcat gtctagataa tatctagata ggcgggaaat
     5521 agcaagagag cgcagcagaa ggaaaagcga cggcgagccg ccgcactcca aaagggtcgc
     5581 gccttcggcg agtcttccct acttgcgttc ggcggaggcg gaggtggggc ctttcaggaa
     5641 tgcgtcgagg cggaggaatt tgcggccgag agcgccggag taggcggacc actcgggttc
     5701 gccggtatcg acggcgaggg cggcgcggac ggcggcgagc ttggaatcca gatcgtccat
     5761 gtagtggaag gccatggctt cgcggatcat cgggagcttg ggcgagccaa actcgtattg
     5821 tccgtgatgg ctgataagga gatgctggac gacggtcttc aagttcgccg gaaaaccctc
     5881 gatggcgtcc atggcctttg aaaccgtttc cagttccatc acgatgtggc cgaggagctg
     5941 gccttcgacg gtgtaggcaa tggcgcgctc gtagcagagt tcttcaagct tcccaacgtc
     6001 gtgtagcatc gcagcggtca gcagcaaatc aacgtcgagc tccgggtagt gggcggcgat
     6061 ttgctttgag aggccgcaca aaccgatgac gtgttcgagc agtccgccga gataggcgtg
     6121 gtgcatcact ttcgccgcgg gagcgcgctt gtacttggcg gcgatcccag gatccgaaat
     6181 gattccggtc accaacttct tgagccaggg attcccgagc gatcccgcat actccaaaag
     6241 ttgggcgtag agcttgccaa catcttcttt ggtgtgcggg agaaaatcgg cgaggtcgac
     6301 ctcctcgggt tttgcgagcc gaagctgctg aagagaaagc tgcggcttgt tacggtaaat
     6361 ctcgacgcgc gcgttgacct tgacgaaatc gtcgcggctg atgtccttta ccacggcgtc
     6421 gaattgatcc cacatgcggg cttcgatggt gccgctgcgg tcgccgagct cgaggcgcag
     6481 ataaggcttg ccctcgcgcg tactgcgaat ctctttttcg tgaacgagga aataagaagt
     6541 aatgctttgt tccgagttga gatcggtgac gaaggacgtc ttcatgtgcc cgactgttgt
     6601 ccggagcgct tgccgaacaa gagaaatttc ttatggctct tcttctgctt gctgacttcc
     6661 ggagtggcgc ttacttccag aatctcggag cctccgccgc cggcgatgtc ctgataaccc
     6721 ggcttaatca ccacgtagct ctgctcgcgc agggtcttga gatactcgcg catcgcgggt
     6781 tccatgcgcc cgccgtagag cttgtccatg atttcatttt tgactttcgc gagtgcttgt
     6841 tcgccctcgt cgtagtgctc caggacctcc agaaccagga agccttgctt ggtttccatg
     6901 actccggtca gctccttgcg cttcattttg aagaccttgt cctcgagttc cttggaaagt
     6961 tctccgcgct tgtagacgcc gaggaagccg ccctggctct tcgtgctgcc atcggaaagc
     7021 cgcttggcga tttcgccgaa gtcctcgccg tcctgaacgc gcttgagtgc ggtctccgcc
     7081 tttttcttca gatcgggcag atcttccggc ttcttccctt ccgtgttaac cacgatttca
     7141 cgcagcgcca cttgctccgg gcgcacgaat tcagccttgt gcgcttcgta atacttcgtc
     7201 acttcttcat cgctgatggt gatgtgcgag ccgacttcgc tgctgatcac gcgctgggtg
     7261 agcagcgtgc tgcgaatgtt gttcttgaaa tcttcccagt tcattccctg cccggaaacc
     7321 gccttttcaa gctcctccat gctgggcagc ttgttctcaa tgcggatggt atcgagcttc
     7381 ttgatgagct ccggctcgac gctcacgccc atgtccttgg cgcgctgcac caggagggac
     7441 tggtcgacca ggtcgcgcag agtgtgtttc tggcgatcct cgatgtcggt ctgcaactgc
     7501 tccggcgtgc agcgattctg gcattcggat ttggcgtcct cttcggaagt ctggcgcgcc
     7561 ttgttatact ccgagcgcgt gatgatttca ttgttgacgc gcgcgatgat ctcctcgacg
     7621 gtcttgccgc tatctggatt ggacggtttc ggagcgaccg gtttcgcagc aggcttttca
     7681 gattttgggt cgggcgccgc catccccggc ttctccagag tgggcccggc cggtggtttc
     7741 ggctgggggc cttgcggctt gtcctgtgcc gcgagtggca gggcagcgca gagcgcgcca
     7801 ataagaacta gatttcgcat caattccctc acctctaaag tttagccctg cccctgaagg
     7861 ccgagcaata cgtttctcaa agcagcgggg atcgattcac cccgtttgac ttccatccag
     7921 agcacgccgc tgggatcgag tttgatgcct gtacgagagc gtaccacgga gaccagtttg
     7981 gccgggtcca gcgttgtctc gtgatggaac ctgactgcga tgcgggtccc ctggcgttcc
     8041 acggcggaga tgcgcaggcg ctcacacatg gatttgaggg cggcatactc caaaaggttt
     8101 tccacggagg taggcggttt gccgaagcgg tcttcgagct ctttgcggac gtcctgcttg
     8161 tctgcttccg aggcgatcga ggagacgcgc ttgtaggtgc gcaggcggag gttttcgctg
     8221 gggatgtagt cctgcgggat gcgcacgtca aagcccaggc taagcgtggt gcgcaggtcg
     8281 ggtgcgactt cttcgccctt cagcttggag acggcgcgct ccagcatctg gcaatacatg
     8341 tcgaagccga tggcttcgat gtggccgtgc tgctggcggc cgagcatgtt gccggcgccg
     8401 cgaagctcga gatcgagtgc ggcgatgcga aagccggcgc ccagttcgct gaactccttc
     8461 atggcggcga ggcgtttgcg ggcgatgtcg gagagcgagg tgtccggagg gacaagaaga
     8521 taggcgtagg cgcgctgggc ggagcgaccg acgcggccgc gcaactggta gagttccgcc
     8581 agccccagac ggtcggcgcg gttgatcaaa atggtgttgg cgcgcgggat gtccaaaccg
     8641 ttctcaacga tggtcgtcgc gacgaggacg tcggcttcat cgcggatgaa ttttaacatc
     8701 gcgctctcga gttctttttc gaccatctgg ccgtgagcca ccaccacgcg tgccttgggc
     8761 accagcttcg ccaccagggc gccgagcgaa tagatcgatt cgacgcgatt gtgaatgaag
     8821 aagacctggc cgtcgcgagc aagttcggtt tcgatggcgc tttgcaccag ctcttcctga
     8881 aagggggcga ccacggtttg aatggccaga cggtcgcgcg gaggcgtttc aatgacagac
     8941 atgtcgcgaa ggccgacaag cgacatgtgc aacgtgcgtg gaatgggtgt cgcggaaagc
     9001 gcgagcgcgt ccacgttctt gcgcatttct tttaggcgct ctttgtgggc gacgccgaag
     9061 cgctgctctt catcgacgac gagcaggccg aggtcggcga acttgacgtc cttcgagagc
     9121 aggcgatgcg tgccgatgac cacgtccact ttgcctgctt ccaaatccgc gagaatcttt
     9181 ttctgctccg cggcgctgcg gaagcggctg agcatttcga tgcgcgcggg gaaagcggcg
     9241 aatcttttct tgaaggtttc gaaatgctgg aaggtgagga cggtcgtcgg cgcgagaacc
     9301 acgacctgct tgttgtccat cacggttttg aacgccgcgc gcatggcgac ttctgttttg
     9361 ccgtagccga cgtcgccgca aagcaggcgg tccatgggct cggcgcgttc catgtcgcgc
     9421 ttgatgtcgc tgatggcggt gatctggtcg gtggtttcct cgaattcgaa ggcatcctcg
     9481 aattcgcgct ggaaattgcc gtccggtgaa aatgcaaaac cctccgtcgt tttgcgctgg
     9541 gcgtaaagcg cgagaagctg gtcggccatg tcttcgacgg atttgcgtgc gcgcgtcttg
     9601 cgagcggtcc aaacggtgcc gccgagtttg tccagcggcg gatgcgtgcc ttcgagcacg
     9661 cggtaactct gaacgaggtc catacgttcc aggggaacgt agaggcgcgc atcgtcggcg
     9721 tacctcagca gcatgaactc gccgctatgt ccgccggatt cgatctggcg aagtccctca
     9781 aactgcccga tgccgtgatc cacatggacc acataatcgc cggacttcag ttcggcaaaa
     9841 tcgccgaaga aaccggaagt gcgaattttg cggctgggcc gctccaccgt gggtgtgacg
     9901 tcgaaaagat cagcgtggcc gaaaagcgta agccgagcgc cgggaaaggt gacgccttcc
     9961 gcgaagggcg cgcggatcag taacatcgcc gcggattcgt gcgcgccttc ggcggtgaag
    10021 ccagcggcgg catcttccga ttcgccgagc acgtagggca cttcatactc gcggcagatg
    10081 tccgcaaagc gttcgatctc tccggtgctc gcggccgtca agaaaaccgt tccgccggaa
    10141 gccagctggg acttcacgtc ccccatacag gcaacgacat tcccgtgaaa acgcgcgctg
    10201 ggtcgcgagg aaagctcgaa ctgcggagtg gaaccgatcc ccagcgcgag ctgctcgatg
    10261 tgaatttggg acgatttgtc cagcgccgcc accaattccg cttcggacca gaaatagtga
    10321 gaagcggcgg gggagttggc gcgcccgtga cgctcgtaat tttccgtcgc agccgcgaga
    10381 tgcttggccg cggcttcccg caaagtttgc ggctcatcga ggaatacgat gggtcgcaga
    10441 gagctttctg ccagctcaaa aagcgtgctc ggcccgggtt cgctggtggg gccgaagaac
    10501 gagggcgtct cccaggatgc atcattcaga ttcgcctttt ccggcgctgg cacagaccat
    10561 tccgtcaaag gaagaagcgt ggtgcgctgg acgggcgcaa tcgaccgctg cgtacgcggg
    10621 tcgaattcgc gcacggattc cacagtgtca ccgaggagct cgatacgcac gggacgagcg
    10681 gcttccggag agaaaacatc gatgatgccg ccgcgcacgg cgaattgccc gggaagctcc
    10741 accatctccg ttcgcgtgta gcccacggaa cccagatggg cgatcaattc atcgtgggga
    10801 atctcagtgt cttttgccaa agtgcgggtg agataaagat acgctgccgg atcctgatag
    10861 cgccaaagcg ccgcggcaat gggcgcgatc accagcgaaa tctgtccatc cgcgacgcga
    10921 aagagcgtgg ccgcacgccg ttccagaatg tccgcgtgcg ggctttgcga ttcccaggga
    10981 agtctgtcga atgccgggag cgtcgcgacg ccccccgcgg ctcccggaaa gatgccgctg
    11041 aagaagcgca gcgtttccgc gagggcttcg gcacggcgat tggaatccgt gacaaaaaat
    11101 gccggacgtc gcagctcgcg cgtcaaataa gccgcaacga gagccttcgc gacatcgtgc
    11161 aggccggtga tcgagatgtg gctcgcaccg gagcgcagcg cggccagcgc accctccatc
    11221 gccgcgtggc gaagaacggc ctccaatctc tcgcggacgg cgggaagtat catgcgtggt
    11281 cgatcaggat tccgcgcgag aagcgcgcgc cccttcgcgt atcctccgga gaatggccga
    11341 tgtggaatag ccgggcacga cgggaatgga gacgacgcgt ccgccggcgg cttccacttc
    11401 ctcgcggcca acgatttggt cgccgggcca atctccgcct ttcaccagga catccggaag
    11461 gaggcgcgcg atcacttcgc gcggcgtcag atcatcgaag atcaccacgg catccacaga
    11521 ttccaatgcg gcgagaattt ccgcgcgctc gcgttctgag ataacgggcc gtccttcgcc
    11581 ctttagttgc cgaacactcg catcgctatt cagtccgacg atcagcgcat cacccaattc
    11641 gcgcgccagt tccaggctgc ggatatggcc cggatggagc aagtcaaagc agccattcgt
    11701 gaagacgacg cggaggccgt tgcgtttctc gcggccaaac cgcaagatcg cttcttcaat
    11761 tgtcaggact gccgaattca aagggtctcc ctgcgggccg gaaagaagct aaagtgatat
    11821 ccgttaagaa gatagtttat cacgcgcagg aacccccgcg cccctgggcc ggcgactgac
    11881 gatgaaatca cgccaggcca ttcgcgctat tgcgggagtt ctgctctgtg ccgttgggct
    11941 ctggcttgcc ctccccacgc cctatcgcga gcgtaccttt cttttcgacg cggggggctg
    12001 ccgactggaa accacgatcg tggaaaagca agagggagcc tcgcaagggt cggttttgct
    12061 cttccatgga atctccgcca acaaaaaaat tatgtcgtac ctcgcgcggg gctttgccga
    12121 gcagggcctt cgcgtctacg ttccggactt gccgggacac ggacgcacgc cgggaccgtt
    12181 ttcgcccgcg cgcgcggaac agtgcggaga ggcacttctc cgcgaactgc tcgcgcgcag
    12241 gttgatcgat gccgatcgca cgattctcgc tgggcactca atgggtgggg ccatcgccga
    12301 gcgtattgca tcccgtgttt cagtcgcggg attgatcgcc atttcgccgg caccgatgcg
    12361 ggccgtgcac ggagtcacgg cggaaaagct gcttttcagc gatcccccga ctttgccccc
    12421 tcattctctg gtgatggttg gttcgctcga actggagtcc atgcgcggca atgccgccga
    12481 cctggctgcc tcgcgaagcg acgggaccgc gaaatacctg gagattcagg gtgcctcgca
    12541 cgtcagcgtt cttttcaacc acgccgcgat gcgtgcttcg caggagtggt cggcagaaac
    12601 tctgcattta cctcccactg cagcgttgcc ctctcaccgc ccgctcatcg gggcgctcgc
    12661 cggtttctta ggaatcctgc tgattgcagg accattcctc cgggaagtct gcgggaagaa
    12721 caggagcgaa gaaatcgccg cgacggtcgc tgcgatcagt atgacgcggc ttcttctgga
    12781 atttgccgta ggctcggtcc taattgtgct cctcctgcga ttctggattc ctttgaaggc
    12841 gattcgactc tttcagggcg actatcttgc cagcttcctg cttcttctcg gggctgtgct
    12901 gattgtgact cactggagtt ccgtgcgtat ggcactgacg aatccctctc gccacctgct
    12961 ggcagcaggc tttgccggag tcgttctgct gcttctcgcc aaggcctggt tcgatttgac
    13021 gttttacgaa gcatggctga taggcgcgaa gtgggtgcgc ttcccctttt tgctggtcgt
    13081 ccttcttcct taccacatcg ccgaagaagc gctgctcggg cccgtagagc gcggaaagaa
    13141 atggcgcaga ttggcgctcg ctttgagttt aaggctcact acctggggcg cgatgatggc
    13201 aggcattctt ctgctgcaca gcggagaaat cctcatcggg cttctggcct tctatatggc
    13261 tctgtttaac gtcgtacagc ggagtggcat ggacatcgtc cgcgaagaaa ccggttcggc
    13321 agcggcgacg gcggtattcg gtgctatact cctcgcgggc ttttgcctgg tgatctttcc
    13381 cctcacctaa cggcccatca cccaaggacc cttcactaga ggaactcatg gcggggttcc
    13441 gcgcccaggg acggaattga aagcgctcag cgagacacga ccatggctca aatagcgatc
    13501 gtcggcgggg gaccgtccgg cgcaatgtgc ggtgaacaac tcgcgcgcgc gggccacaag
    13561 gtcaatcttt ttgacgagca cctggcttgg gaaaaaccct gcggcggcgg cctgacgcac
    13621 aaagccattc agtgtttccc tttcctgctt gataattcct atcctaaaaa actggtgaat
    13681 tctgtcgagc tgatcagcag cgaagagcat cgcgccattc tcgaaatgcc ccatccgatc
    13741 gtgatctatt cacgaacggt gctcaacggc atgctgctgg atcgcgcacg ggaggcgggt
    13801 tgcaacgtgc agcgttcccg cgtgatgagt gtggacacga caaccgcgaa gccgcgctac
    13861 tgcgtggaag gcgattggca ggaagccgat tttctggttc tcgcggctgg tgcgcgaaat
    13921 caactgctcc ccgaaacacg ggcgttgcaa cgcgacgaac tggagatgac ccagggttac
    13981 ttcgtgccgc agaccagcga ctccatcacc atcaagttcc tgcggaactt tgagggctat
    14041 atctggtcgt tcccgcgctg cgatcatctc tcggtcggca tttgcggcag catgtcgtcg
    14101 cataccagca cggagctgcg cggccatctg caaacattcg tggagaagca cggcatacag
    14161 acggaaggcg caaagttttt cagccacgtc ctgccttcgc cgaaagagcg cacgctttcc
    14221 gagcgaaatg tcatcggtaa gaactgggcg ctcgtcggag atgcggcggc ctgggtcgat
    14281 cccctcacgg gtgaaggact gttctacgct attcgctccg gcgaattgct gggccgctcg
    14341 ctcgccgaag gctgcccgga gaaatatccg gcttgggtga aggctacttt ctgcgccgaa
    14401 ctggaattcg cggcacgcat cgtgcggcga ttctatcgtg gatctttcct gggtagcgcg
    14461 gtcaccactc gcatggttca gtttctgcgc cgcagccccg tgttccgaca attgatgggc
    14521 gatctcttca gcggcacgca ggactacacc agcctgaagc ggagactgtg gggccatctg
    14581 gggatcaccg tcagcgaatt tatctccagc gtcttgagcc tcgatcgacc cgccaggacc
    14641 agcgtgcctc gcacgggaac cgcgggcgac tgaagatagc tgctccatcc gatccgttct
    14701 agcaatatag tcatacggac accgtcggca tctaaagtcc gggtacagtt cgtccctcta
    14761 atcatggcca ccgagacagc atccaaatca ccagctaaga gtgaaaaacg caaaaacgcg
    14821 tggaagaatc tccctgacgt ctgggcgctg atgaagccga gacgcgggct gctggcgctg
    14881 ggcttcgtgc tcatggccgt caatcgcctt tctggattgg ttttgcccgc ctccacgaag
    14941 tatctggtgg acaacgtcat cggcaaccgg caaatccagc tgctgacgcc gatcgtgctt
    15001 ggcgtcctcg cagcgacggt cctgcaggga ctgacttcgt ttacgctgac tcaattgctc
    15061 tccaagtccg cgcaaaaaat gattgccgac atgcgccgcc aggttcaggc gcacatcgga
    15121 cggcttccgg tggcgttcta cgatgccaac aaaaccggcg cgcttgtttc gcggatcatg
    15181 agcgacgtcg aaggcgtccg caatctggtc ggaacaggtc tggtcgaatt tgtcggcgga
    15241 ctcatgaccg cggttatcgc gctcgtctac ctgcttcata ccagcgtcac catgaccgcc
    15301 gtcgcgtttg ccatcctgct ggttttcgga tacggcatca acaaagcgtt tacgacgatt
    15361 cggccgattt tccgcgcacg gccgaaaatc aacgcggaag tcacgggacg cctcaccgaa
    15421 tcgctgagc
//
LOCUS       DASYVO010000299        27881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_275495, whole genome shotgun sequence.
ACCESSION   DASYVO010000299 DASYVO010000000
VERSION     DASYVO010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 27881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27881
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275495"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VGR03_09920"
     CDS             <1..430
                     /locus_tag="VGR03_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994637.1"
                     /translation="DTVEITAIYALAGALAPGNANGEIPIEDLRDGLREKQRQTLSAG
                     VRARVPRLGTQIHGGYKWLSGRTVSRVDGYGEALFQDDPYLHVSVRQALPRLGLGRWE
                     ALAECQNLLEQGYVPVAGHNGSAMLVPAFRTFRGGLSWQF"
     gene            619..3441
                     /locus_tag="VGR03_09925"
     CDS             619..3441
                     /locus_tag="VGR03_09925"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1994638.1"
                     /translation="MKQNLRVSLGAVMLALATLAAVIFAAYNFDQRTRFETPEDGVVW
                     LDTDHGVQAWRVAPNSPAARAGIRAGDRLVAINDALISRDVQVTEKLWRAGLWSQERY
                     RVARNGQEFETRLVTAPAEKPASIENYLRLVGLLYLFIGLFIFVRRWSAPRAVHFYIF
                     CLVSFVLYSFHYTGKLNAFDWVIYWSNIAARLLAPALLLHFALVFPERTEGRGWTAAK
                     LAGVYALPAVLVFTHVSVAVNVLGFVPWLGARFVLDKIELTYLGICFFAAGIVFYRSY
                     RHASPGVLRQQLKWLTGGTIFGSAPFALFYIAPFVFDLASATWSKFSALSLVFIPLCF
                     GYAIIRYRLMDVDIIFKRGLAYTAATAGVVGVYFAVVALIGELLHLKWPTGPGGGIIA
                     IVVAAFLFQPFRDWTQARLDRFFYRDRLDYRRTLIEFGRTLTNEVRLEPMLGSVMDRV
                     SQTLLVDRLAIFVEDPVQPGQMRLARSMGVRLSEPLDLSFLEPARPEFARGALFFESP
                     RGTRNLPDAVRRSLEQLDLNYYVPCRIREHTVAVLGLGKTVDGDYLSSDDVELVETIA
                     GYVAIALDNSHLYTSLEQKALEIARLKDFSENIIESLSVGVLAVDLEGVVESWNSRME
                     QLIGASRRDAVGRPLAALLPPELAAEILARAGDDQISGIYKHRLHHRGQTYTLNVSIT
                     PLVSKSGEKIGLLLLFDDVTQRERMEGQMSRTEKLTSLGLLAAGVAHEVNTPLAVISN
                     YIQMLAKQMPEGDPRQAIIEKIVKQTFRASEIVNNLLNFSRVGAAEFGEVDVNRLVEE
                     TLSLVAHPMKTSRVQIVKQLGESLPPVLGSANKLQQVFLNLFLNAKDAMPGGGMLEVR
                     TAARNGSVEIEIADTGAGISREDIHRIFDPFFTTKSSGRGTGLGLSVSYGIIQEHSGK
                     IDVRSTPGKGTSFHLEFPAARKAVHA"
     gene            3434..4906
                     /locus_tag="VGR03_09930"
     CDS             3434..4906
                     /locus_tag="VGR03_09930"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV1994639.1"
                     /translation="MPKGSILVVDDESEIREGLELLLSSEGYGVASAETGESGLAKLE
                     EHPYDLLLLDVSLPDRNGLELLREIRRRDPHLSVVLITAYGSIDMARQAFKGGALDYI
                     TKPWSNDELLAQVAQAVESRRLREENVQLKRALKQRFNFPNIIGKSEKMLSLLDLVSQ
                     VAPSRSTVLISGESGTGKELIAKAIHSASPRSDKTFVPVNTGSIPVDLLESQLFGHVR
                     GAFTSAIASKKGLFEVADQGTIFFDEIATISPETQAKLLRVIQEREFMRLGGTEQIKV
                     DVRIVAASNVDLLTLVREGRFREDLYHRLNVIHLRLPPLRDRREDVPILLVHFLERYC
                     RENGKGARQFSQAAMKLMMDHDWQGNVRELENVVERAVVLSLQERMDVDLLPENIRSK
                     EIVRGVRLQLSAFAPLAGEPGARLGADNAQPSLFQVMDEVERRIIADMLARTGWNQTE
                     AAERFQIPLSTLNQKIKRLGIDVRRRGRGGEDIHSVSHGK"
     gene            5015..6346
                     /locus_tag="VGR03_09935"
     CDS             5015..6346
                     /locus_tag="VGR03_09935"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1994640.1"
                     /translation="MIVRLSSQSARLTLVCVSVVLAGSLAYFSIRNARAQYLAASQAP
                     PGLDRAVQLEPGNARNWFLLGHYWQYNLEQRDTARAITFYKIALSLDPHSPSTWLDLG
                     EASETEGDLKSARAAFLQAKENYPISAEVAWRYGNFLLRQGELPAAYREIYRSLTAEP
                     GRAAAAVSVCWRAAPEIQPILDSALPASRDVYLSAIAVLVQEQETVAALAVWKRLAIL
                     RPKLQMEDVAKFLEVLVAKGDIADAREVWAQSLSFAGISRPDDPAGSLIWDGGFESDV
                     NVGFGWRIQPVNGAQVTFDASVKHSGSRALRVRFDGTQNVNFGHICQSVAVEPGSSYR
                     FSAWMRADTLTTDRGVLFLLSTPELPGTPPAMTPELRGTEPWTRVTLPLRTSKDVHIV
                     QVCLARMPSGKLDNKIAGTVWVDDVSLLPDTLPDPVSGDARPVDLRKKERP"
     gene            6343..7590
                     /locus_tag="VGR03_09940"
     CDS             6343..7590
                     /locus_tag="VGR03_09940"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV1994641.1"
                     /translation="MKFLRGGICFLAAFGVVAHGGVEDWAAAVLEAGAAVLLLVWAVR
                     CLLSKEEKLYVPVLLPPLLLFAAVGLAQLLLHRTVYAYETRISLQLLGAFLILLFLAG
                     QVYRTVADWRGFVWFVLVLGFAVSVFGILQHLTFNGKLYWFREMSYGGIPFGPYVNRN
                     HFAGLMELVIPIALTPLVFGKVRRERWFLVGLFAVLPIVALFLSASRGGIIGFVVELV
                     FLGILLGLRRSGARHALAAGLVLLMAVFLVSWLGVEHALERFASLQPLEVNSSKRAAM
                     ARDTWHIFLDHPLTGTGLGTLQTVFPKYDTLYDGKVVNHAHNDFLEALAESGVLGGIG
                     CAWFLILLAARGLGNLLKTENGAAAALQGGGLVGCTGFLVHSLVDFNLHIPANALLFL
                     LLANLATAGVPPRHPLADPAIRP"
     gene            7633..8829
                     /locus_tag="VGR03_09945"
     CDS             7633..8829
                     /locus_tag="VGR03_09945"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEV1994642.1"
                     /translation="MLQPGKLGAAKWYLYRAPSAGMQGVLLCLLTALFGGPAARAQQP
                     VLETPEQTNERIRAFSIATQKVHREYVIGPGDLLSVEVFDVDELTRDTRVSQTGKIAL
                     PLIPIRLHVAGLTEIQTEQKLAEVLEANGLVSHPQVSVTVKEKKSKPITVVGAVTHPM
                     VYQAERQVTLLEVLSEAGGIANDAGNVVIVTRAAPDPQADSKARDTAAEAKGDAASDP
                     DGTSTAAASSPGASADGSAPVISAPEPAALPPTRITIGLSDLLEDGDLKNNIVLQAGD
                     IVTVPHAGIVYVIGAVERPGGFVLSNDRAHMSTLKVLSLAGGLRRTANAERAVIIRKD
                     ASGHQQQVAVNLKKVLARKSEDVTLNPSDILFIPESGGKRVALKAAEIALALGSAAAV
                     FRLAQP"
     gene            8837..11176
                     /locus_tag="VGR03_09950"
     CDS             8837..11176
                     /locus_tag="VGR03_09950"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEV1994643.1"
                     /translation="MQDNESLIPRESGSKATIEVIPASSRPAVWDLSPREPHLYDYLL
                     ILRKHQWLILTFLLAVVTIVSIATFRMRPVYVATARIEVDRESQNFMPFQGADPYAYL
                     IDQENYIETQSKILTSETLALQTIRSTGLGGNRDFSPGGKVSEAIMSGSLANQKRPPE
                     LAAFLGSLSVKRVPNSQLLDVSFESTDSQLAARVLNMHLENYIEQNFRSRYEATTQAS
                     TWLADQLNELKFKVEKSEDARITYERQNSIWTLDDKQSITTQRLSDINKELTAAQSER
                     MKKEAVYQLAKSGSFDVIPEARESGILQDVMKRRTEVFTEYAAALNQYGPNFPRVQRL
                     QAQVKEFDQLIETEKKNVMNRTENDYRAARQRELLLTEALDRQKAETNQQAERLVQYN
                     ILKREAEANKQLYEGLLTKLKEAGISAGLRSSNIRVVDPAMIPSSPARPAKTRNIALS
                     FLVGLVGGIGLALMREYLDNTIKTPDDIELIAKLPSLAVVPAFDGANRKSRAMRFLRG
                     APVNGNQAHIELFSHHLPKSQISEAFRALRTALLLSQAEHPPQVILVTSALPREGKTT
                     AAANLAVTLAQLGDRTVLVDGDLRKPGVSRVLSLTNEKYAGLSSYLAGVSNLDLVTIQ
                     HPTIENLSAIPTGPLPPNPADLLSSHRMKEAIAELRKRYKFIVIDTPPILAVTDAVIL
                     SVQADGVLLVVRSGETSKEAFMRTRDLLASVKSRVLGVVLNAVDSSSPDYHYSYRYYP
                     YSYGYGTDHSTESVSEQAALSQPVTSHPAHEDEENTSAL"
     gene            11186..12190
                     /locus_tag="VGR03_09955"
     CDS             11186..12190
                     /locus_tag="VGR03_09955"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEV1994644.1"
                     /translation="MRYLITGGAGFVGSHLADQLLGRGDSIALLDNLSTGSMENIRHL
                     KNSDRLEYHLDSIENKQLLAELVDEADVVVHLAAAVGVRLIVESPVRTIETNVNGTQL
                     ILEAACKKRKLVVAASTSEVYGKSTHVPFREDADLVLGPTTKGRWSYAASKALDEFLA
                     LAYWKEKQLPVIVARLFNTVGPRQTARYGMVLPNFVGQALDGKPITVYGSGKQSRCFC
                     DVRDTVEGLIRLMDTERAIGEVVNIGNTEEISIEGLARLVKERTNSSSEIVYVPYDRA
                     YEPGFEDMPRRVPSIEKLHELTGFRPKTSLNEIVDRVTAHFREKRAATERRSVVGSRA
                     "
     gene            12340..13836
                     /locus_tag="VGR03_09960"
     CDS             12340..13836
                     /locus_tag="VGR03_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994645.1"
                     /translation="MNTRSYEDSLVEADRTRNASARRANLILLGLFVLSLPLVNPWVR
                     GDGVGYYAYARALLIERRLDFTQDYLHANQRFREARLDERNRPRPDFRTATGRLENHF
                     SVGPAILWSPFLLAAHAGALLARSLGSSVAADGFSAPYRIAMSFATALYGFLALLFSF
                     RLACRYAEWKWAFLATVGVWGASSLPVYMYFNPSWSHAHSAFTVAIFLCYWHKTRGGQ
                     TFLRWLLLGGLAGLMLNVYYANAMVLVAPGVEALLEYAKRLRGPTRDWAGAARLVAVH
                     TAFLMVILVCLLPTFITRQIIYGAPFQSGYIPLKNWLWSAPALLPVLFSSNHGLFSWT
                     PILLPASAGLLLLAFYRPNVGGPLLAAAIVFYSFVACYPNWDGISSYGNRFFVSLTPL
                     FILGLAVFLQRLASAFGRKHAVIPISVLLVGLFVIWNIGFMFQWGMHLVPARGPISWN
                     EMVHNQFVAVPRQVGESIRSYLFHRSALMKSIERKDLEQWRDSPENKP"
     gene            13971..15536
                     /locus_tag="VGR03_09965"
     CDS             13971..15536
                     /locus_tag="VGR03_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEV1994646.1"
                     /translation="MNRSSSIAPVPTLEDASACGPTGELRRNDSAASKASRLSLGTRV
                     FQNTIVLLGGRALSLLLSAVASILLARYLGRERMGEYGALYAYLALYSWLSTFCLEQI
                     LAREVAGRRSEAAVIFHTGSMVGLSFALAGAILAPLLAPLFGYTGQLRWLIAVAALDL
                     LILPPIRLPGIVFQVDMRQWFSVGIGLVRQVLWLAAIVLLAMRNAAFYQVIVVRTICG
                     ILEAGLVLKLCYRPGMVTGPRRFDLTEARRLLRYAFPLAMSAISVGVFYRIDQVMLHN
                     MTGDRALGPYVVAAQLTEQFGMLPVALISSLFPVLVQTVHQEEQFRHYLDVSYRFLMS
                     VVFFACAVVTPVTAPLVSLLYGKEFLPTAGLINVLIWSEVPLFLGVALTNALIAKNLQ
                     RYLPYSTAVGAVMNIGLNLMFIPRWGALGAAWATVISYAVATVLFYLVFQPARAMTWQ
                     GLRIALPPFAVALAITWALRHLPVHFAAKILIATLLYAVGAWLTGTVRRAEIKRLWEI
                     FRGALRFSPQETL"
     gene            15676..16563
                     /locus_tag="VGR03_09970"
     CDS             15676..16563
                     /locus_tag="VGR03_09970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1994647.1"
                     /translation="MHRVSVYIPAYNAGEFIARAIEGVLAQSLAPDEILVIDDSSTDA
                     TRDIAARYAGVKLVRHDGNRGLAATRNTAFRSARNEFVASLDADCVAEPGWLEHLVPH
                     LSDPRIAGVGGRLIEGVQRTAADRWRRAHMPQEWGEEPLRNPRFLFGCNNLFRKSAVL
                     QAGGYDESMRTNGEDIDLSQRLREHGGELVYEPAARATHLRHDTIHSILETYWRWWRF
                     GVRAYANGVRLRSVLGHAVFVHFRHTFLEFVRRDLRARRLDLLGLDLLALGYFPYRDF
                     RLWLDAQSGGAPRRISSEI"
     gene            16567..17655
                     /locus_tag="VGR03_09975"
     CDS             16567..17655
                     /locus_tag="VGR03_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV1994648.1"
                     /translation="MRILITGGCGFIGSRLALHFAEQGHRVVAMDNLVRRGSEGNIGR
                     LQKHGVEYLHGDVRNAEDFANLPAGIEFICDASAHPSVVTGYANPSYDVSNNALGVIR
                     VLEYARQHRCPLIFWSSNRVYNADRLNALPRRETATRLEWDAEAWRKLSTEERPRGFD
                     PVHGISEEFSVDGGQRSIYGTSKLMADVACQEYAQAYDMPVVVNRFAVISGGGQFGKA
                     EQGWLAWWAVAHWFGLPLKYIGWKGKQVRDILFIEDVCRLADLEMKQISRFRSEVFNA
                     GGGAANSLSLIEATRELEKRIGRPTSIVEEDAPRKADLAVYFTDNRKVERILGWRPQT
                     GLDRGLKEILEWIRENEAELRALYCSRG"
     gene            17679..18749
                     /locus_tag="VGR03_09980"
     CDS             17679..18749
                     /locus_tag="VGR03_09980"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV1994649.1"
                     /translation="MAQKTVLVTGSSGLIGSQAVEHFDRQGCLVVGVDNNMRRVFFGA
                     AGDTTWNLERLKRGTKNFVHANLDIRDRPAMDDLFRTHRFHLIVHCAAQPSHDKAREI
                     PLLDFEVNALGTVNLLEAARQHAPEVVFALLSTNKVYGDAPNEIPLKELETRYDYARV
                     EDFDGIDETCRIDRTLHSLFGASKAAADLVAQEYGLYFGMNVGIFRGGCLTGPSHSGV
                     ELHGFLSYLVKVALSGGTYSVFGYKGKQVRDNIHSYDVVRAIEEFAANPRPGEVYNLG
                     GGRANSVSILEAIGKIEELTRRKLAWKYIAEPRKGDHICYISDLKKFQSHYPNWRITV
                     GLDEILEQIISARTQQLRAAGV"
     gene            18798..19871
                     /locus_tag="VGR03_09985"
     CDS             18798..19871
                     /locus_tag="VGR03_09985"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV1994650.1"
                     /translation="MDRRKIRRKLRTAQRVRREQGWGALARIALSRLSYPVLQARIRY
                     VNAERGRPVPQTVQIEASSNCNLRCPSCSLSREVNPGRHLSPEELGVILDRLPFQPKA
                     VSLNGIGEPLINPHFLRLVDILGERGIDCTFFTNGTLLVPHLREAILKRHNVSYVGIS
                     CDGARKETFEVLRLGAKFDTWKQFVKEFLAAAHGRAERRVETVMSSVVSRRNLGEVGE
                     IVELAAELGFRSVQFADVMPNDEVAASLALTDADRQHFDLNALQRRGQELGVGVLAHM
                     RRKKRPPKAGLNCFQPWEYMMISAEGDVLPCCAIVGSDKALVMGNILHQSFSTIWDGK
                     EFQSFRRTSADGTNPLCSICPYY"
     gene            19884..20951
                     /locus_tag="VGR03_09990"
     CDS             19884..20951
                     /locus_tag="VGR03_09990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1994651.1"
                     /translation="MSRPFLTVLVDTYNHERFIEQAIQSVLEQDFSASDREILVVDDG
                     STDRTPEILRRFEPQVRVLRKANGGQATAFNAGIPACRGEIIAFLDGDDWWAHSKLSK
                     VSSIFAENRELGIVGHGITEVFPNGSQRTECLPHPARFQLNSREGALLFRARKQFLGT
                     SRMTVRARLLETLLPVSEDLRIEADEFLFTVAALHSPVMILTEALTFYRLHGGNLYQM
                     AGSTKDGLLRKQRVLVALAISIGERFRTEGVPQEIAEAVIEIIQAEADQLRLTLKGGF
                     PWETVRTERTIYRVMHENPPLGHRIFRFLTMIPAALLPSSWFYLIRRRLAANDQYNRL
                     RGILAPVPKPVHVHRSEKTHV"
     gene            20948..21682
                     /locus_tag="VGR03_09995"
     CDS             20948..21682
                     /locus_tag="VGR03_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1994652.1"
                     /translation="MSLRSGYDVWHQRVFDADPDHDDASSPWYQLVREAIGPVAGLRI
                     LEVACGRGGFVRELARAGAHVTGCDFSFAALRVGQHKLNRAGNGISASLLQGDAQSLP
                     LADDWFDLVVSCETIEHLPDPQIAVQEMHRVTRPSGKLFLTTPNYCNFMGVYELYAKF
                     RHPGRKDDQPLDRRQWFPQIRRCVHRAGWTILRTDGTVHQFPFLPGRNAIRFERVESN
                     PVVRRFLSPLALHYFVIAQKKEAATA"
     gene            21679..22821
                     /locus_tag="VGR03_10000"
     CDS             21679..22821
                     /locus_tag="VGR03_10000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1994653.1"
                     /translation="MMKVLLAACVPRRREGGVAGVVTSYAQGLEKRGHSVTTLFSEDL
                     LQPNEGRGRIAELRFASRLARFITRRHTEFSVVNLHAPAGVLYGMKRWFLGTSGPPYV
                     MTLHGLEENRIHAMKREARKGRAWHFSGKNRVWHSVYHQPRFDWAIRTADAAHCFSRD
                     VWTLLRLKYDLDDDRVAYIPNGVAERFFIERDYGSRSTLGLLYAGTWLDQRGLFYLRE
                     ALPRIFAALPNLRLTFAGPGVPATEILNFFGQAVSARLDILETIPWEKMPQLYADHDI
                     LLFPSIMEGQPSVVLEAMATGMPVVTAETCGMVDAIEHGWDGWLVPPADSAAIEDAVL
                     QLAASVELRQRIGRAAQDRMRRQTWDRAVYSFEKLLERTISNAQRK"
     gene            22823..24499
                     /locus_tag="VGR03_10005"
     CDS             22823..24499
                     /locus_tag="VGR03_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEV1994654.1"
                     /translation="MLSLGINYSQMHDSSACIVRDGELLFAIAEERISRVKHDAGFPH
                     NAIRACLEFAKAKPSELDDVCFGWQAPGPFFLHDLKCYATRRMPLSYLNLLNSTLYFL
                     SVRHQGGGAKRFTQRFGPTRARMRFVDHHLAHALSAYAYSGFEEAAVVVMDGRGAWEA
                     SSIWHGRGGRLEHILTIPWPNSLGLFYAEFTQYLGFQKYSDEWKLMGLAPYGRPGVNL
                     REFIHPEDAPHWVNTRLLGESASGSSGGIAALLGPERVPESEINDRHKDIAYAVQEAC
                     ETAMMGLVRQAIKMTGCRDVCLAGGVALNSKANGKIAASGLVGRMFIQPAAADDGAAL
                     GAALAPYLDDNGRVPNHAMRHAYLGDSFDDTAVESALKTYKISYTHVDDPAKAAAELL
                     ARGKILGWFQGRMEFGPRALGSRSILADPRDPEMNAKVNQAVKFREWWRPFAPSILAE
                     KACDYLESAADSPFMILTAQVRPEKRAVIPAVTHVDGSARPQTVEKETNPLYWRLIDE
                     FGKRTGVYVVLNTSFNLRGEAIVHTPTDAIRTFFSSGMDALVIGSFLVEK"
     gene            complement(24686..24775)
                     /locus_tag="VGR03_10010"
     tRNA            complement(24686..24775)
                     /locus_tag="VGR03_10010"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24739..24741),aa:Ser,seq:tga)
     gene            complement(24814..25176)
                     /locus_tag="VGR03_10015"
     CDS             complement(24814..25176)
                     /locus_tag="VGR03_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994655.1"
                     /translation="MSPDTFATPRRSPRVFHRMRMRAEGRSEEGRKFRENCETVVVNV
                     HGGLLYLKHEVRNGELLVLANPETQEEQECRVVYLGSEVEKGQRVGVEFLTPSPHFWG
                     IDFETGPTSVDSGDSSAH"
     gene            25416..26780
                     /locus_tag="VGR03_10020"
     CDS             25416..26780
                     /locus_tag="VGR03_10020"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV1994656.1"
                     /translation="MNLAEHIESRREQHLQELCEFLRIPSISAKSEHKPDIERAAKWV
                     AGNLRGAGFKSVEIVPTQLHPLVYAESLEAPGKPTILFYGHYDVQPAEPLELWTTPAF
                     EPTVRDGKLFGRGTADDKGQVHIHLKAFEALHRMNGRLPINVKVLIEGEEEVGSVSLW
                     DFVQKNQAKLKADALVVSDTSMLSRGVPSITYGLRGLNYYQIEITGPERDLHSGVYGG
                     AVPNPLTILAELFAELHDKNFRVTVPGFYNDVAKLSAAERKALSALPWSVKEFKKAVG
                     APAYCGEKGFSIVEQLWTRPTLELNGVWGGYLGEGAKTVIPSKAWAKFSTRLVPHQEP
                     EKIARLVERHLRRLLPKTVKLNFETLSTGKPWVAPFHAPIFLKAQAALEKGFGRKAVF
                     IREGGSIPFVTQMYDTFKVPCVLMGFGLPDENAHAPDEHIALENYFGGIKAIAHFYED
                     LATL"
     gene            27003..27296
                     /locus_tag="VGR03_10025"
     CDS             27003..27296
                     /locus_tag="VGR03_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV1994657.1"
                     /translation="MAAKPMTKSKVVASIAEKCETSKKTAALFFEELFNLAVRECKGA
                     AGKFVIPNIGRAVKAHRKARMGRNPATGEAIKIKAKTVVRLRAAKAFKDAVLK"
     gene            27602..>27881
                     /locus_tag="VGR03_10030"
     CDS             27602..>27881
                     /locus_tag="VGR03_10030"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1994658.1"
                     /translation="MSTPESLPRAWELLQEAYQAQMEGEYERAVDLYQASLKLHPTAE
                     AHTFLGWTYHFLGHLEDAIAECKKAIEVDPDFGNPYNDIGAYLLEMGRY"
BASE COUNT         5366 a         8022 c         8764 g         5729 t
ORIGIN      
        1 cgatacggtg gagatcaccg cgatctacgc gctggcagga gctcttgctc cggggaacgc
       61 aaacggcgag attccgatag aagacttgcg cgacgggctg cgggagaagc aacggcagac
      121 gctctctgca ggcgttagag cgcgagtccc gcggctgggc acgcagattc atggaggata
      181 taaatggctc agcggccgaa ccgtctcgcg agtggatggt tacggtgagg cgcttttcca
      241 ggacgatccc tatctgcatg tgagcgtccg acaggcgctg ccgagactcg gactgggtcg
      301 ctgggaagcc ctcgcggaat gccaaaacct gcttgagcag ggatatgtcc cggtagctgg
      361 acataatggc agcgctatgc tggtccccgc attccggacc ttccgaggcg gcctaagctg
      421 gcagttctga ggcccaatac ggggctttgg aatcttgtat ccaacctacc agaaaccgga
      481 gacccaaagg attccaggtg cggcgtcttt ccatgtttat gaaaacatga gccttgtgaa
      541 taataagaga taccaaacga tggaaccaca cttgctctca tttcttgaag gttcgtcccg
      601 atgggtgtgg tgcgggttat gaagcagaac ctccgcgtaa gcttaggtgc ggtcatgctg
      661 gcgctggcaa cgctggcggc ggtgatcttt gccgcgtaca acttcgacca acgcacgcgc
      721 ttcgagacgc ctgaggatgg cgtggtctgg ctggacacgg atcacggagt gcaggcgtgg
      781 cgagttgccc cgaattcacc ggcggcccgg gcgggaatcc gtgccggaga ccggctggtt
      841 gccatcaacg atgcgctgat cagcagagac gttcaagtca ccgaaaagct gtggcgcgcg
      901 gggctgtggt cgcaggaacg ctaccgggtt gcgcggaacg gacaggagtt cgaaacgcgc
      961 ctggtgacgg cgccggcaga gaagccggca tccatcgaga actacctgcg gctggtcggg
     1021 ctgctgtatc tgttcatcgg gctgttcata ttcgtgcgcc ggtggagcgc gccgcgggcc
     1081 gttcacttct acatattctg tctcgtatcg tttgtcctct actccttcca ctacaccgga
     1141 aaactcaacg cgttcgactg ggtcatctat tggtcaaaca ttgcggcgcg gctgctggcg
     1201 ccggcgttgc tgctgcactt cgcacttgtt ttcccggaac ggacggaagg gagaggatgg
     1261 accgcagcga aactggccgg agtctacgcc ctgccggccg ttctcgtgtt cacgcacgtg
     1321 agcgtcgcgg tgaacgtcct ggggtttgtg ccgtggctcg gtgcgcggtt cgtgctcgac
     1381 aagatcgagt tgacctatct gggcatttgt ttcttcgcgg cgggaatcgt cttctaccgg
     1441 agttaccgac atgcgtcacc gggcgtactg cggcagcaat tgaaatggct gacgggggga
     1501 accatcttcg gaagcgcgcc gttcgcgctt ttttatatcg cgccttttgt gtttgatttg
     1561 gcgtcagcca cctggtcgaa gttttcggcg ttgagcctgg tgttcattcc gctgtgtttc
     1621 ggctacgcga tcatccgcta ccggctcatg gacgtggaca tcatcttcaa gcgcggcctg
     1681 gcttatacgg cggcgacggc gggggtcgtg ggagtgtact tcgccgtggt ggcgctgatc
     1741 ggggagttgc tccacttgaa atggccgaca gggccgggag gagggatcat cgcgatcgtc
     1801 gtggcggcgt ttctcttcca gcccttccgg gattggacgc aggctcggct cgaccggttc
     1861 ttctatcgag accggctgga ctaccgccgc acgctgatcg aattcgggcg cacgctgacg
     1921 aacgaagtga ggctcgagcc gatgctgggg tcggtgatgg accgcgtgtc gcagacgctg
     1981 ctggtggacc ggctggcgat ctttgtggaa gacccggttc agcctggaca gatgaggctg
     2041 gcgcggtcca tgggggtgcg cctgagcgag ccgctggatt tgagtttcct cgagccggcc
     2101 cggcccgagt tcgcgcgcgg ggcgctcttt ttcgagtcgc cccgcggcac gcgcaacttg
     2161 ccggatgcgg tgcggcgttc cctggagcaa ctggacctga actactacgt tccgtgccgc
     2221 atccgggagc acaccgtggc ggtgctgggt ctcggcaaga cggtggacgg agactacctt
     2281 tcgagcgacg acgttgaact ggtggagacg atcgcagggt atgtggccat cgcgctggat
     2341 aattcgcacc tgtacacgtc gctggaacaa aaggcgttgg agatcgcgcg gctcaaggat
     2401 ttcagcgaga acatcatcga atcgctgagc gtcggcgtgc tcgcggtgga tctggaaggc
     2461 gtcgtcgaat cttggaattc gcggatggag cagctcatcg gcgcatcgcg gcgggatgcg
     2521 gtggggcgcc cactggccgc gctgcttccg ccggagctgg cggcggagat tctggcgcgc
     2581 gccggagacg accagatcag cggcatctat aagcaccggc tccatcatcg aggacagacc
     2641 tacaccctga acgtctccat cacgcccctg gtgagcaagt cgggagagaa aatcgggctg
     2701 ctgctgctgt ttgacgacgt cacgcagcgc gagcggatgg aaggtcagat gtcgcggacc
     2761 gaaaagctca cttcgctggg gctgctggcc gcgggagtgg cgcacgaagt gaatacgccc
     2821 ctggcggtga tctccaacta catccagatg ctggcgaaac agatgccgga gggggacccg
     2881 cggcaggcga tcatcgagaa gatcgtgaag cagacttttc gcgcaagcga gatcgtgaac
     2941 aacctgttga atttctcgcg ggtcggagca gccgaattcg gcgaagtgga cgtcaaccgg
     3001 ctcgtggaag agacgctttc cctggtggcg catccgatga agacgtcgcg ggtgcagatc
     3061 gtgaagcagc tgggagaatc gctgccgccg gtgctcggtt cggcgaacaa actccagcag
     3121 gtgtttctga acctgtttct gaatgcgaag gacgccatgc cgggaggcgg gatgctggaa
     3181 gtgcgcacgg cggcgcgcaa cggaagcgtg gagatcgaaa tcgccgatac cggcgcgggg
     3241 atttcgcgag aggacattca ccggatcttc gaccccttct ttacgacgaa atcgagcggc
     3301 cgcggcacgg gactcggcct ctcggtgagc tacgggatca tccaggaaca ttccgggaag
     3361 atcgacgtgc gctcgacgcc gggcaagggt acctcgttcc atcttgagtt ccccgcagca
     3421 cggaaagcgg ttcatgccta agggctcgat tctggtcgtg gatgacgaat ccgagatccg
     3481 cgagggtctc gaactcctcc tgagctcgga aggatacggt gtggccagcg cggagaccgg
     3541 ggagtccggg ttggccaagc tggaagagca cccctatgac ctgctgctgt tggacgtgag
     3601 tcttcccgac cggaacggct tggagctcct ccgcgagatc cggcgccgcg atccgcatct
     3661 ctccgtcgtc ctgatcaccg cctacggctc aatcgacatg gcccggcaag cgttcaaagg
     3721 cggcgcgctc gattacatca cgaagccctg gtcaaacgac gaattactgg cgcaggtggc
     3781 gcaggccgtc gagtcacggc ggctgcgcga agaaaacgtg cagctcaagc gggcgctgaa
     3841 gcagcgcttc aattttccca acatcatcgg caagagtgaa aagatgctgt ccctgctcga
     3901 cctggtgagc caggtggcgc cttcccgctc caccgtgttg atttccggcg agagcggcac
     3961 gggcaaggaa ctgatcgcga aggccatcca ctcggcgtcg ccgcggtcgg acaagacctt
     4021 cgtgccggtg aataccggtt cgatccccgt ggatctgctg gaatcgcagc ttttcggcca
     4081 cgtgagggga gcattcacaa gcgcgatcgc gtcgaagaag gggctttttg aagtcgccga
     4141 ccaggggaca attttctttg acgaaatcgc cacgatcagc ccggagacgc aggcgaagct
     4201 gctccgcgtc atccaggagc gcgagttcat gcggcttggc gggactgaac agatcaaagt
     4261 ggatgtgcgg atcgttgcgg catccaacgt ggatttgctc acgctggtga gggaggggcg
     4321 cttccgcgaa gacctgtatc accgtcttaa cgtcattcac ctgaggctac ctccacttcg
     4381 cgaccgccgc gaggatgtgc ctatcctcct ggttcatttt ctggagcggt attgccggga
     4441 gaacgggaag ggggcgcggc aattcagcca ggcggcgatg aaattgatga tggatcacga
     4501 ctggcagggg aatgtgcgcg agcttgagaa tgtggtcgag cgcgccgttg tcctgtcgtt
     4561 gcaggaaaga atggacgtgg acttgctgcc agagaacatc cgcagcaagg agatcgtgcg
     4621 cggtgtgcgg ctgcagctct ccgcattcgc gccgctggcg ggtgaacctg gcgcgcggct
     4681 tggcgcggac aatgcacagc cgtcgctttt ccaggtgatg gatgaagtcg agaggcgcat
     4741 catcgcggac atgctcgcac gcacgggctg gaaccagacc gaagcggctg agcgctttca
     4801 aatccccctt tctactctca accagaagat caaacgtctg ggaattgatg tgcggcgccg
     4861 cggccggggt ggcgaggata tccacagcgt ctcccacggg aagtgagtgc aaccgcgagc
     4921 agctcgctga ttcgggcgcg gacagaagcc cgttccacac aacaggaata ctttgtgctt
     4981 gcctccacgg tggggtctag gcagattaac ccggatgatt gtccgcctat ctagccagag
     5041 tgcgcggcta acgctggttt gcgtttcggt ggtgcttgcg ggaagcctgg cctatttcag
     5101 tattcggaat gcccgggcgc aatacctcgc tgcgtcgcag gcccctcccg ggctcgatcg
     5161 cgccgtgcag cttgagcctg gcaacgcgag gaactggttt ctgctgggcc actactggca
     5221 atacaacctg gagcagcggg acactgcacg ggccatcacc ttctacaaga tcgcgctttc
     5281 ccttgatcct cactcgccgt cgacctggct cgatctgggc gaagcctccg aaaccgaagg
     5341 cgacctgaaa tccgcgcgcg cggcgtttct ccaggccaaa gaaaattatc cgatctccgc
     5401 cgaagtggcc tggcgctatg gaaattttct tctacggcag ggtgagctgc ctgccgcgta
     5461 cagagagatt taccgtagcc tgacggcgga gcccggacgc gcggcggcag ccgtatcggt
     5521 ttgttggcgc gccgcgccag agatccagcc cattctggac tctgcgcttc ccgcttcgcg
     5581 cgacgtatac ctgagtgcga tcgctgtgct tgtacaagag caggagacgg tagcagcgct
     5641 cgccgtctgg aagaggctcg cgattttgcg cccgaagcta caaatggaag acgtcgcaaa
     5701 gtttctcgag gtcttagtgg cgaaagggga tatcgcggac gcgcgcgagg tctgggcgca
     5761 atcgttgtct ttcgccggaa tttcgcgccc ggatgatcca gcgggctcgc tcatctggga
     5821 tggaggattc gagagtgacg tgaacgttgg attcggctgg cgcatacagc cggtgaacgg
     5881 agcgcaagta accttcgacg cgagtgtgaa acattccggg agccgcgcat tgcgcgttcg
     5941 cttcgacggg acgcagaacg tgaacttcgg gcacatttgc cagtccgtgg ccgttgagcc
     6001 ggggagctcg taccggtttt cggcctggat gcgggcagat acgctgacaa ccgatcgcgg
     6061 agttcttttt ctcctgtcga caccggagtt gccggggacg ccccctgcga tgacgccgga
     6121 actgcgaggc acggaaccgt ggacgcgcgt gacattgccc ttgaggacca gcaaggacgt
     6181 tcacattgtc caggtttgcc tggcgcggat gccgagcgga aagctggaca acaagatcgc
     6241 cggaacggtc tgggtagatg atgtctcgct gttgccggac acgctgccgg acccggtcag
     6301 cggcgatgcg cggccagtgg atctacgaaa aaaggagcgg ccgtgaagtt ccttcgcgga
     6361 ggcatctgtt ttctggcggc cttcggagtg gtggcacacg gaggtgtcga agattgggcc
     6421 gcggcggttc tcgaagcagg agcggccgtc cttctgctcg tgtgggcggt gcgctgcctg
     6481 ctgtcaaagg aggagaagtt gtatgttccg gtgctcctgc cgccgctcct gctgtttgcg
     6541 gcagtgggtc tagcccaatt gttgcttcat cggacggtct atgcctacga aacgcgaata
     6601 agtctgcaac tcctgggcgc attcctgatc ctgctgttcc tggcaggaca ggtgtaccgc
     6661 accgtcgcag attggcgggg attcgtgtgg tttgtgctgg tgctggggtt tgccgtctcg
     6721 gtttttggaa tactgcagca cctgactttc aatgggaaac tgtactggtt ccgtgaaatg
     6781 agctacggcg ggatcccgtt tgggccctac gtgaaccgaa accatttcgc gggtctgatg
     6841 gagctagtga tcccgattgc gctgacgccg ctggtgttcg ggaaggtgcg gcgtgaacgc
     6901 tggttcctgg ttggactttt cgccgtgttg ccgatcgttg cgctctttct ctcagcatcg
     6961 cggggaggaa tcattggttt tgtggtggag ctggtcttcc tggggatact cctggggctg
     7021 aggcgcagcg gcgcgcgcca tgccttggcg gcagggctcg tgttgttgat ggcggtgttt
     7081 ctggtctcgt ggctgggcgt ggagcatgcg ctggagcggt tcgcttccct ccagcctctg
     7141 gaagtgaatt cgagcaaacg agccgcgatg gcgcgagaca cctggcacat cttcctggac
     7201 catccgctga ccggcaccgg cctgggaacc ctgcagacgg tgttcccaaa gtacgatacg
     7261 ttgtatgacg gcaaggtagt gaatcacgca cacaacgact ttctcgaagc cctggcggag
     7321 agcggcgttc tcggaggaat cggctgtgct tggtttctca tcctgcttgc cgcgcggggc
     7381 ctcggcaacc ttctgaagac ggaaaacggg gcggcagcgg cgcttcaggg gggagggctt
     7441 gtgggttgca ccggatttct ggtgcatagc ctggtggatt tcaacctgca tatcccggcg
     7501 aacgcattgc ttttcctttt actggcgaat ctggcgacgg caggagttcc cccccgacac
     7561 cctcttgcag atcccgcaat tcggccttaa aaagttctgg tcccttgtat actgtggacg
     7621 gggtcatccg tcgtgttgca gccaggaaaa ctgggtgcgg cgaagtggta tctttaccgc
     7681 gcccccagtg ctgggatgca gggcgtattg ctctgtctcc tcaccgctct tttcggaggg
     7741 ccggcagccc gtgcgcaaca acccgttctc gagacgccag agcaaaccaa cgaacgcatc
     7801 cgggcctttt ctatcgcgac gcaaaaggtt caccgcgaat atgtgatcgg gccgggcgac
     7861 ctgctctcgg tggaggtatt cgacgtagat gaactcacgc gtgatacgcg cgtcagccag
     7921 acgggcaaga tcgccctgcc gcttattccc attcgacttc atgtagccgg actgaccgag
     7981 atccaaaccg aacagaagct cgccgaagtg ctggaagcga acgggctcgt ctcgcacccg
     8041 caagtgagcg tcacggtgaa ggagaaaaag agcaaaccga tcacggtggt cggcgcagtc
     8101 acccatccga tggtctatca ggcggagcgt caggtgacgc tgctggaagt gctttcggaa
     8161 gcggggggca tcgcaaacga cgcgggaaat gtggtgatcg tgacgcgcgc ggcgcccgat
     8221 ccccaggcgg attcgaaagc tcgtgacact gccgcagagg cgaagggcga tgctgcaagc
     8281 gatccggatg ggacgtcaac tgccgcggcg tcatcgcccg gcgcgtccgc cgatggaagc
     8341 gccccggtca tttccgcgcc ggaacccgcg gctctgccgc ccacccggat cacgatcggg
     8401 ctctctgacc tgctggagga cggcgacctg aaaaacaata tcgtgctcca ggccggagac
     8461 atcgtcaccg ttcctcacgc gggcatcgtc tatgtgatcg gagcggtcga gcggccaggc
     8521 ggctttgttc tttcgaatga ccgcgcgcac atgagcactc tcaaggtgct ctcgctggcg
     8581 gggggcctgc ggcgcacggc caatgccgag agggcggtga ttatccgcaa ggacgcctcg
     8641 ggacatcaac agcaagttgc cgtaaatctg aagaaggtgc tagcgcggaa gagcgaagac
     8701 gtaacactga atccgagcga cattctattc attccggaaa gcggcgggaa acgggtcgcg
     8761 ctcaaggctg cggaaatcgc gctcgccctc ggcagcgcag cagccgtctt tcgcctggcc
     8821 cagccttagg aaggatatgc aagacaacga gagcctcatt ccccgggaga gtggatcgaa
     8881 ggccacgatc gaggtgattc cggcgagcag ccggccggcc gtctgggacc tgtccccgcg
     8941 cgagccccac ctctacgact acctgctcat tctgcggaag caccagtggc tgatcctgac
     9001 gtttctgctg gccgtggtga ccatcgtttc catcgccacg tttcggatgc gaccggtcta
     9061 tgttgcaacc gcgcggatcg aggtagaccg ggaaagtcag aatttcatgc cgttccaggg
     9121 ggcggacccg tacgcctact tgatcgatca ggagaactac atcgagacgc aatccaagat
     9181 cctgacgagc gaaactcttg ctcttcagac gatccgcagc accggtctgg gcggaaaccg
     9241 ggatttctct ccggggggca aggtttctga ggccatcatg agcggcagtt tggcgaatca
     9301 gaagcggccg ccggaacttg ctgcttttct gggaagcctg agtgtgaagc gggtgccgaa
     9361 cagtcaatta ttggacgtct ctttcgagtc caccgattct cagctcgcgg cgcgcgtttt
     9421 gaacatgcat ctcgagaact acatcgagca gaatttccgc agccgctacg aagccacgac
     9481 acaggcttcc acttggctgg cggaccagct caacgagctg aagttcaaag ttgaaaaatc
     9541 cgaggacgcg cgcatcacct acgagcggca gaactcgatc tggacgcttg acgacaagca
     9601 aagcatcacc acccagcggc tgtcagacat caataaggag ctgacggcgg cgcagagcga
     9661 gcgcatgaag aaagaggcgg tgtatcagct cgcaaaatcc ggcagctttg atgtgatccc
     9721 ggaggcgcgg gagagcggaa ttctgcagga cgtcatgaaa cgccgcaccg aggtctttac
     9781 cgagtacgcc gcggccctca accagtatgg accgaatttc ccgcgggtgc agcggctgca
     9841 ggcccaggtg aaagaattcg accagctgat agagacggaa aagaaaaacg tcatgaaccg
     9901 cacggagaac gattatcgtg cggcgcggca gagagagctt ttgctgaccg aggcccttga
     9961 tagacagaaa gcggaaacca atcagcaggc cgaacgcctg gtgcagtaca atatcttgaa
    10021 acgcgaggcg gaagcgaaca aacagctcta cgaagggctg ctgacgaaac tcaaggaagc
    10081 gggaatttct gcgggcctcc ggtcgtcgaa tatccgcgta gtggacccag cgatgatacc
    10141 gtcgagcccc gcccgtccgg cgaagacccg caacatcgcg ctttcgttcc ttgtcgggtt
    10201 ggtcggaggc atcggtctgg ccctcatgcg cgaatatctc gacaatacca tcaagacacc
    10261 ggatgacatc gagttgatcg cgaagcttcc ctcactggcg gtcgtgcctg cgttcgatgg
    10321 cgcgaaccgc aagagccggg caatgcggtt tctgcgggga gcgcctgtga acgggaatca
    10381 ggcgcatatc gagctcttct cccatcatct tcccaagtca cagatttcgg aggcgttccg
    10441 cgcgttgcgg acagctctcc tgctctctca ggcggaacac ccgccgcaag tcattctggt
    10501 gaccagcgcg cttccgcgcg agggcaagac taccgccgca gccaacctcg cggtgacgct
    10561 cgcgcagttg ggggatcgaa ccgtgctcgt ggatggggac ctccgcaagc ccggcgtcag
    10621 ccgtgtactc agcctgacga acgaaaagta tgcgggcctg agctcgtacc tagcaggcgt
    10681 ttcaaacttg gatctggtta cgattcagca cccgacgatc gagaatcttt cggccatccc
    10741 gacggggccg ctgccgccga atccggccga tctgctttct tcacaccgga tgaaggaggc
    10801 catcgcggaa ctgcggaaaa ggtacaagtt cattgtgatt gacacgccgc cgatcctggc
    10861 tgtgacggac gccgtcatac tatccgtcca ggcggacggc gtgctccttg tggtgcgcag
    10921 cggtgaaact tccaaggaag ccttcatgcg cacccgggac ctcctggcca gcgtcaaaag
    10981 ccgcgtgctg ggtgtcgttt tgaacgcagt ggattcaagc tcacctgact atcactattc
    11041 ttaccgctac tatccttatt cgtatggcta cggaacggac cactcgaccg agagcgtctc
    11101 ggagcaggct gcactttcgc agcccgttac cagccatcct gcgcatgagg acgaagaaaa
    11161 cacgtcggct ctctgaggag gcttcttgcg gtatctgatc accgggggcg ccggatttgt
    11221 cggctcgcac cttgccgacc aacttctagg gcgcggggat tccatcgccc tgctcgacaa
    11281 tctctccacg ggcagcatgg agaacatccg gcacctgaag aattcggatc ggctggaata
    11341 tcatctcgat tccatcgaga acaagcaatt actggctgag ttggtggacg aggccgatgt
    11401 ggtagtgcac cttgctgcgg cggtgggcgt tcggttgatc gtcgaaagcc ccgtgcggac
    11461 gatcgagaca aacgttaacg gcacgcaatt gattcttgag gcggcttgca agaagcgcaa
    11521 gctggtggtg gcggcctcga cctccgaagt ctatggaaaa agcacccacg tgccgtttcg
    11581 cgaggatgcc gatctcgtgc tcgggccgac gaccaagggc cgttggagct acgccgcgtc
    11641 caaggcgctc gacgaattcc tggcgctggc ctactggaaa gaaaaacagc tacccgttat
    11701 cgtggcgagg ctatttaaca cggtcgggcc gcggcagacg gcccgctacg gcatggttct
    11761 tccgaacttc gtgggtcagg cgctggatgg taaaccgatc acagtctacg ggtcgggcaa
    11821 gcagtcacgc tgcttctgtg acgtgcgcga cacggtggaa ggactgatcc gtctgatgga
    11881 caccgagcgc gccatcggag aggtcgtgaa catcggcaac acggaagaga tttcgatcga
    11941 ggggctggcc agactggtga aagaacgcac aaacagcagc tcggagattg tctatgttcc
    12001 ctacgaccgg gcgtatgagc cgggctttga ggacatgccg cgtcgagtgc ccagcatcga
    12061 aaagctgcat gaattgacag gctttcggcc aaaaacttcg ctcaatgaaa ttgtggaccg
    12121 tgtgacggct catttccgtg aaaaacgcgc agctacagag cggcgaagcg tagtgggcag
    12181 ccgggcgtga gtcacaacca gcgcgatcgg actggctcca catgaggcgg aggaagtgcg
    12241 cgcgagttcc gctcatccgg tgaaagacac gcctgaattt ggaattgaac cggagatttt
    12301 gggtccgaat cggagcgcct ctaattcacc tgcctctgaa tgaacactcg ctcctacgaa
    12361 gattccctgg ttgaggcaga ccgaacaagg aatgcgagcg cgcggcgggc caacctcatt
    12421 ctcttgggcc tgtttgttct ttctctcccg ctggtgaatc cgtgggtacg cggagatggt
    12481 gtcggttact atgcgtatgc gcgggcgctg ctcattgaac gccgtctgga tttcacgcag
    12541 gactacctcc acgcgaacca gagatttcgt gaggcgcgtc tcgatgagag aaaccggccg
    12601 cggcctgact ttcgcaccgc tacggggcgc ctcgaaaatc acttctcggt aggaccggcg
    12661 atcctgtgga gcccatttct gctcgctgcg catgcagggg cgctcctggc gcgatcgttg
    12721 ggctcgtccg tggccgccga tggtttttcg gcgccttacc gcattgccat gagttttgcg
    12781 acggcgctct acggattcct ggctctgttg ttctcttttc gcctggcgtg caggtacgcg
    12841 gaatggaaat gggcatttct cgccacggtc ggcgtctggg gggcaagctc cctccccgtt
    12901 tatatgtatt tcaatccatc ctggtcacac gcgcactctg ccttcacggt cgccattttc
    12961 ttgtgctact ggcacaagac gcggggaggc caaacctttt tgcggtggct cttgctgggg
    13021 ggtctggccg gactgatgct taatgtgtat tacgccaatg cgatggtctt ggtggctccg
    13081 ggtgtcgaag cgcttcttga gtatgcgaag cgcttgcgcg ggccaaccag ggattgggcg
    13141 ggagcggcgc gcttggttgc cgtacacacg gcctttctta tggtcattct ggtctgcctc
    13201 cttcccactt tcattacgcg gcagatcatc tacggagctc ccttccagtc agggtatatt
    13261 ccgctgaaaa actggctctg gagcgcgcct gcgctgctgc ccgtcctctt ttcgtccaat
    13321 cacggactct tttcctggac gccgattctc ctgccggcct cggcaggcct cctcctcctt
    13381 gcgttttatc ggccgaacgt gggcggcccg ctgctggcgg cggcaattgt cttctactct
    13441 tttgtcgcgt gctacccgaa ttgggatggg atctcttcct acggcaatcg tttctttgtt
    13501 tcacttacac cgctctttat tctcggcttg gcggtttttt tgcaaaggct agcctccgcg
    13561 tttggccgta agcacgcagt aattccgatt tcagtcttgt tggtggggct ttttgttatt
    13621 tggaatatcg gctttatgtt tcagtgggga atgcacttgg tcccggcgcg cggacccatt
    13681 tcttggaacg agatggtgca caaccagttc gtggccgtgc cgaggcaagt cggagaaagt
    13741 attcgcagtt acctttttca tcgaagcgct ctaatgaaat ccatcgaacg gaaagacctg
    13801 gagcaatgga gagacagccc ggagaacaaa ccctaggcag attccaggcg ggccgcagcc
    13861 agaggaattt gatgcacctt cggcggacgg aaaagccaaa tagatgcaca acagaagaca
    13921 tgaagatgtt gggccgatgt tgcctttggt aatctaacag acgagtggaa atgaatcgtt
    13981 cgtcatccat cgcccctgtg ccgactctcg aagacgcatc cgcgtgcggg cccacgggag
    14041 agcttcgcag aaacgattcc gccgcttcga aggcaagccg gctgagcctc ggcacgcgtg
    14101 tctttcaaaa caccatcgtg ctgctcggcg ggcgggccct cagcctgctc ctctcggcgg
    14161 tggcgtccat tctgcttgcg cgttacctgg gccgggagcg catgggggag tacggcgctc
    14221 tctatgctta tctggcgctg tattcctggc tctcgacgtt ctgcctcgag cagattctgg
    14281 cgcgcgaagt tgctggcagg cgctccgagg ccgccgtaat ctttcacacg ggctcgatgg
    14341 tcggcctgtc ctttgctctt gcgggagcga tcctggcccc tctgctggcg ccgctttttg
    14401 gctacaccgg gcagttgcgc tggctgatcg ccgtcgcggc actggatctg ttgatcctcc
    14461 ctccgattcg cctgccggga atcgtctttc aggtggacat gcggcagtgg ttcagcgtgg
    14521 ggatcggcct tgtccggcaa gtcttgtggc ttgcggcgat cgtgctgctc gcgatgcgga
    14581 acgcggcctt ctatcaagtg atcgtggtgc ggacgatttg cgggatcctg gaagcggggc
    14641 tcgtcctgaa gctctgctat cgtccgggaa tggtgacggg gccgcggcgg ttcgacttga
    14701 ccgaggcgcg acgcctcctg cggtacgcgt ttcctctggc gatgagcgcg atttccgtgg
    14761 gtgtgtttta ccggattgac caggtgatgc tgcacaacat gacgggcgac cgcgcgctcg
    14821 gtccgtacgt cgttgccgcg cagctcaccg aacagttcgg aatgctgccg gtggccctga
    14881 tctcttctct cttccccgta cttgtgcaga ccgtccacca ggaagagcag ttccgtcatt
    14941 atctggacgt gagctaccgt ttcctgatgt cggtcgtatt tttcgcatgc gcggtggtca
    15001 cgcccgtcac ggcaccgctg gtctcgctgc tgtacggaaa ggagtttctt ccgacggcgg
    15061 gtctgatcaa cgtgctgatc tggtcggaag tgccgctctt cctgggcgtc gctctgacga
    15121 acgcgctcat cgcgaaaaac ctgcaacgat acctgcccta ttcgactgcc gtgggcgcgg
    15181 tgatgaacat cgggctgaac cttatgttca ttccgcgatg gggcgcgctg ggggcggcct
    15241 gggcaacggt catcagttat gcggtcgcga ccgtgctctt ctatctggtt tttcagccag
    15301 cgcgggcgat gacctggcag ggactgcgca ttgcgctgcc gccatttgcg gtggcgctgg
    15361 ccatcacgtg ggcgcttcgg cacttgccgg tccacttcgc cgcgaagatt ctgattgcca
    15421 cgctgctcta tgcggtgggg gcttggctga ccgggaccgt ccgccgcgcc gaaatcaagc
    15481 gcctttggga gattttccgc ggtgcattgc gtttttcccc gcaggagacg ctgtgagcat
    15541 tcaccacacg gtgccttatg tccgtcactg acgctgctga gttccattgc tggtatgcgc
    15601 cggaccgtgg agccgccttg cagaaaggcc gctttatcgt ggcggatcgg aaccgcgaaa
    15661 caaacgaggc gcggagtgca tagggtaagc gtctacattc ctgcttacaa cgcgggcgag
    15721 tttatcgccc gggcgatcga gggcgtgctc gcgcaatcac ttgccccgga cgagatcttg
    15781 gtcatcgacg acagttcgac cgatgccacg cgcgatatcg ccgcgcgcta cgccggcgtg
    15841 aagctcgtgc gccacgacgg caaccgcggc ctggctgcca cgcgcaacac cgcgttccga
    15901 agcgcgcgaa acgagttcgt ggcctcactg gatgccgact gcgtggccga accaggctgg
    15961 ctcgaacatc ttgtgcctca cctgagcgat cctagaatcg caggggtcgg tggccgcctg
    16021 atcgaaggcg tgcagcgaac agcagcggac cgttggcgcc gtgcgcacat gcctcaggaa
    16081 tggggtgaag agccgctgcg caacccgcgc ttcctcttcg gctgcaacaa cctcttccgc
    16141 aagtcggcgg ttcttcaggc ggggggctac gacgaaagca tgcgcaccaa cggcgaggat
    16201 atcgatcttt cgcagcggct tcgggaacat ggcggggaac tggtctacga gccggccgcg
    16261 cgcgcaaccc atctgcggca cgataccatc cactcgatcc tggagacgta ctggcgctgg
    16321 tggaggttcg gagtccgcgc ctatgcgaac ggcgtgcggt tgcgttcggt tctgggccac
    16381 gctgtcttcg tgcatttccg ccatacgttt cttgagtttg tgcggcggga tctgcgggcg
    16441 cgccgcctcg atttgttggg tttggacctg ctcgctctcg gctattttcc gtaccgcgat
    16501 ttccggctgt ggctcgatgc gcagtccggt ggcgccccgc ggcgtatttc ttcggagatc
    16561 tgactcgtgc gcatcctcat caccggcggc tgcggattca tagggtctcg gctcgcgctg
    16621 catttcgccg agcaggggca ccgcgtggtg gcgatggaca accttgtccg ccgggggagc
    16681 gagggcaata tcgggcggct gcagaagcac ggcgtggaat acctgcatgg agatgtgcgc
    16741 aacgccgagg atttcgcgaa tctgccggcg ggaatcgagt ttatttgcga cgccagcgcg
    16801 catccgtcgg tggtcacggg ctacgcaaat ccgagctatg acgtgtcaaa taacgcgctc
    16861 ggcgtgatcc gcgtgctcga atatgcgcga caacatcgct gcccgcttat tttttggagc
    16921 tcgaatcgcg tttataacgc cgaccggctg aacgcgttgc cgcgccggga aacggcaacc
    16981 cggctcgagt gggatgccga ggcctggcgg aagctctcaa ccgaagagcg gcctcgcggg
    17041 ttcgatccag ttcacggcat ctccgaagag ttcagcgtgg acggagggca gcggagcatt
    17101 tatggcacga gcaagttgat ggcggatgtt gcatgccagg agtatgcgca ggcgtacgac
    17161 atgcctgtcg tcgtgaaccg gtttgccgtc atctccgggg gcgggcagtt cggtaaagcg
    17221 gagcagggct ggctggcctg gtgggcagtc gcgcactggt tcggcctgcc gctgaaatac
    17281 atcggctgga agggcaagca ggtgcgcgac attctattca tcgaagatgt ctgccgcctg
    17341 gcagacctgg agatgaagca gatctcccga tttcgcagcg aagtgttcaa tgccggcgga
    17401 ggagcggcga actcgctttc tctcatcgag gccacgcggg aacttgagaa gaggattggg
    17461 cggcccacct cgattgtgga ggaagatgca cctcgcaaag cggatttggc cgtgtatttc
    17521 acggacaacc ggaaagtgga aagaattctc ggctggaggc cgcagacggg cctggaccga
    17581 ggtttgaaag agatcctgga gtggatccgt gagaatgagg cggaactgcg cgctctctac
    17641 tgttccaggg gataattcgc aaggctgagt ggtcctcgat ggcacaaaaa acagttcttg
    17701 taacgggcag cagcggattg atcgggtcgc aggcggtgga gcatttcgac cgccagggct
    17761 gccttgtcgt cggtgtggac aacaatatgc gccgggtatt tttcggcgcg gccggcgaca
    17821 cgacctggaa cctcgagcgg ctgaagcgcg ggacgaagaa tttcgtccac gcgaatctgg
    17881 acatccgcga ccgccccgcg atggacgatc ttttccgcac gcaccgattc catctcattg
    17941 ttcattgcgc cgcgcagcct tcgcacgaca aagcgcggga aattccgctg ttggattttg
    18001 aagtgaacgc gctcggcacg gtgaacctcc tggaagcggc tcggcagcat gctccggaag
    18061 tggtgttcgc cttgttgagc acgaacaagg tttatggcga cgcccccaac gagatcccgc
    18121 tgaaggaact ggagacgcgc tacgattatg cgcgagtgga ggatttcgac gggattgacg
    18181 aaacctgccg gatagaccgc acactgcatt cccttttcgg ggcttcgaaa gccgcggcgg
    18241 acctggtcgc gcaggaatac gggctgtatt tcgggatgaa cgtgggcatt ttccgcgggg
    18301 gctgcctgac ggggccttcc cactccggag tagagctgca tggttttctt tcctatctgg
    18361 tgaaagtcgc gctgagcggt ggaacgtatt ccgtcttcgg ctataaaggg aagcaagttc
    18421 gtgacaacat tcacagctac gatgtcgtgc gcgcaattga ggaatttgcg gcgaatcccc
    18481 ggcccggcga agtctacaac ctgggcggag gccgcgcgaa cagtgtctcc attctcgaag
    18541 ccatcgggaa gatcgaagag ttgacgcgca ggaaactcgc gtggaaatac atcgcggaac
    18601 cacgcaaggg agaccacatc tgctacatca gcgacctgaa gaagtttcag tcgcactacc
    18661 cgaattggcg gatcacggtc gggctcgacg agatcctcga acagattatt tccgcgcgga
    18721 cgcagcagct tcgcgcggcg ggagtgtaat ggaccgaaga atgagatttg cgaagccagg
    18781 gggcagagga attgacaatg gatcgtagaa agattcgacg aaagctgcgc actgcccagc
    18841 gggtacgcag agagcagggc tggggcgcgc tggcgcgaat cgccctcagc cgcctcagct
    18901 accctgtgct ccaggccaga atccggtacg taaatgccga acgcggaagg ccagttccgc
    18961 agacggtgca aatcgaagcg tcaagcaact gcaatctccg atgcccgtct tgctcgcttt
    19021 cgcgcgaagt caatccgggc cggcatcttt ctccggaaga gcttggcgtc attctggacc
    19081 gcttgccgtt ccaaccaaag gcagtttccc tcaacggtat cggcgagccg ctcatcaatc
    19141 cccatttctt gcgattggtg gacatcttgg gcgaacgggg aattgattgc acatttttca
    19201 caaatgggac cctcttagtt cctcatcttc gcgaagcgat cctgaaaaga cacaacgtct
    19261 cctatgtcgg gatttcctgc gacggcgcgc gtaaagagac gtttgaggtg cttcgattgg
    19321 gagcaaagtt cgacacctgg aagcaattcg tcaaggaatt tcttgctgcc gcccatggac
    19381 gggctgagcg acgcgtagaa acggtaatga gcagcgtcgt cagccgccgg aatctcggcg
    19441 aggtgggcga gattgtcgaa ttggctgctg aactcggctt ccgctccgtt caatttgccg
    19501 atgtcatgcc gaacgacgag gtcgccgctt cgcttgcgtt gacggacgca gatcgccaac
    19561 attttgactt gaacgcattg caacgacgcg ggcaagagct gggggtggga gtgctcgcgc
    19621 acatgcgccg caagaagaga ccgcccaagg cagggctcaa ctgcttccag ccctgggagt
    19681 acatgatgat ctccgctgaa ggggacgttc tgccctgctg tgccatcgtc ggctcagata
    19741 aggcgctggt catgggaaat atcctgcacc agagtttttc caccatctgg gatggcaagg
    19801 agttccaaag ctttcggcgg acatctgcag acgggacaaa tccgctctgc tccatctgtc
    19861 cctattactg aggtgcaaga gcgatgagcc gacccttcct tactgttctc gtggatacgt
    19921 acaatcacga gcggttcatc gagcaagcga tccagagcgt gctcgagcag gatttttccg
    19981 caagtgatcg ggagattctt gtggtggacg acggctcgac cgatcgcacg ccggagattc
    20041 ttcgccgttt tgagccgcag gtgcgcgtgt tgcgcaaggc gaacggcggc caggcaacgg
    20101 ccttcaatgc ggggattccc gcatgccgcg gcgagatcat cgcgtttctg gatggagacg
    20161 actggtgggc tcattcaaag ctcagcaaag tcagctccat ttttgcggag aatcgggagc
    20221 taggcatcgt gggccatgga atcacggaag tcttccccaa cggcagccag cggacggaat
    20281 gtctgccaca ccctgcccgc ttccagttga attcacggga aggagcacta ctctttcgcg
    20341 ccagaaagca atttctgggc acgagccgga tgaccgtccg cgcgcgactt ctcgaaacgc
    20401 ttcttccggt gtcggaggat ctcaggatcg aggcggatga atttctgttc actgtggccg
    20461 cgctccattc tccagtgatg attttgaccg aagcgttgac gttttaccgg ctccatggcg
    20521 gcaatcttta tcagatggcc ggctcgacga aagacgggct gctccgcaaa cagcgtgttc
    20581 tagtggcgct ggccatcagt atcggcgagc ggtttcggac cgaaggcgtc ccacaggaaa
    20641 tcgcagaagc cgtcatcgag atcattcaag ccgaagcgga tcaattgcga ctcacgctga
    20701 agggcggatt tccttgggaa actgtccgca cggaacggac gatctaccga gtgatgcacg
    20761 agaatccgcc gttgggacat cgcattttcc gttttctcac gatgattcct gcagctctcc
    20821 tcccctcttc gtggttctat ttgatacgac gacgcctggc cgcgaacgac cagtacaatc
    20881 ggctgcgcgg aattcttgcc ccggtgccga agccggtgca cgttcatcgt tctgagaaga
    20941 cacacgtatg agcctgcggt ccgggtacga cgtctggcac cagcgagtct ttgacgcgga
    21001 cccggatcat gacgacgcgt cgagtccttg gtaccagctc gtgcgggagg cgattgggcc
    21061 ggtggcgggg ttgcgaatcc tggaggtggc ttgcggacgg ggcggattcg tgcgcgagct
    21121 ggcccgggct ggcgcgcacg tgacgggctg cgatttctca ttcgccgcgc tgcgcgttgg
    21181 ccaacacaaa ttgaacaggg ctggaaatgg catctcggcg agcctcctcc agggtgatgc
    21241 gcagagcctg ccgctcgccg atgattggtt cgacctggtg gtttcttgcg agacgattga
    21301 gcacctgccc gaccctcaaa tcgcggtcca agagatgcat cgcgtcacgc ggcctagcgg
    21361 caaattgttt ctgaccaccc caaattattg caatttcatg ggcgtatacg aactgtacgc
    21421 aaagtttcgt cacccagggc gcaaggatga ccagccgctt gatcgtcgcc agtggtttcc
    21481 gcagattcgc cgttgcgtcc accgcgccgg ctggacgatc ttgcgcaccg atggcaccgt
    21541 ccaccagttc cctttcctcc ccgggcgcaa cgccattcgt ttcgagcggg tcgagtccaa
    21601 tccggtagtc cggcgttttc taagcccgct ggcgctgcac tatttcgtga tcgctcaaaa
    21661 gaaggaagcc gcaaccgcat gatgaaggtc ttgctggccg cgtgtgtgcc ccggcggcgc
    21721 gaaggcggcg ttgccggcgt ggtcacaagc tacgcgcaag gcctcgaaaa gcgcggccat
    21781 agtgtcacca ctctgttttc agaggacctc ctgcagccga acgagggccg agggcgcatc
    21841 gcggagctaa gattcgcatc gcgtctcgcc aggttcatca cccggcggca cactgaattt
    21901 tctgtcgtga atcttcacgc ccccgcgggg gttttgtacg gcatgaagcg ctggtttctg
    21961 ggcacctccg gcccaccgta cgtgatgacc ttgcacgggc ttgaggaaaa tcgcattcac
    22021 gcaatgaagc gggaagcccg caagggtcgc gcctggcatt tctccggaaa gaaccgcgtc
    22081 tggcacagcg tttaccacca gccccgcttt gactgggcca ttcgcaccgc tgacgcggcg
    22141 cactgcttca gccgtgatgt ctggacgctc ctccggctga aatacgatct cgacgacgac
    22201 cgggtcgcct acatcccgaa cggcgtggcg gaaaggtttt tcatcgagag ggattatggt
    22261 tcccggtcaa cgctggggct tctctatgcg ggcacatggc tcgaccagcg tggcctcttt
    22321 taccttcggg aggcgctccc gcgaattttc gccgcgctgc caaatttgcg attgaccttc
    22381 gcgggtccgg gcgtccccgc gacggagatc ctgaatttct tcgggcaagc ggtctccgcg
    22441 cgcctcgaca ttctcgaaac gatcccttgg gaaaagatgc cgcaactcta tgccgaccac
    22501 gatattctgt tgttcccgtc gattatggag ggacagccgt ccgtggtttt ggaggcgatg
    22561 gctaccggca tgcccgtggt aacggcggag acttgtggaa tggtggacgc aatcgagcac
    22621 ggctgggacg gctggctcgt gcccccggcg gattccgccg cgattgagga tgcagtcctc
    22681 cagttggctg cgtcggtcga actacggcag aggattggcc gcgcggcgca agaccggatg
    22741 cgccggcaga cgtgggacag agccgtttac agcttcgaaa agctccttga gcgcacaatt
    22801 tccaatgcgc agaggaagtg acttgctgag cttgggaatc aactattcgc agatgcatga
    22861 ctcttcggcg tgcatcgtgc gcgacggtga gttgcttttc gccatcgcgg aagaacgcat
    22921 cagccgcgtc aagcacgacg ccggcttccc gcataacgca attcgcgcct gtctggaatt
    22981 cgcaaaggca aagccctccg agctcgacga tgtgtgtttc ggctggcaag cgcccgggcc
    23041 tttcttcctc cacgacctga agtgctacgc gacgcgccgc atgccgctca gctatctcaa
    23101 tctgctcaat tccacccttt attttctgag cgtccggcat cagggcggag gagcgaagag
    23161 gttcacgcaa cgcttcgggc caacaagggc gcggatgcga tttgtggatc atcatctcgc
    23221 tcacgccctg agcgcctatg cctattccgg gtttgaagaa gctgccgttg tcgtgatgga
    23281 cggccgcggc gcttgggaag cgagttccat ctggcacggt cggggaggcc gccttgagca
    23341 catcctcacc atcccgtggc caaattcgct cggcttgttt tacgcggaat tcacgcaata
    23401 ccttggattt cagaaataca gcgatgaatg gaagctgatg ggcctggcgc cgtacggccg
    23461 gccgggcgtg aatttgcgcg aattcattca tccggaggat gcgccccact gggtaaacac
    23521 ccggcttctc ggagagagcg catcgggttc cagtggcggc atcgcggcgc tcctcggtcc
    23581 ggagcgcgtg cccgaaagcg agatcaacga ccggcataaa gacattgcct acgccgtgca
    23641 ggaagcctgc gaaacagcga tgatgggcct cgtgcggcag gccatcaaaa tgaccggctg
    23701 ccgcgacgtg tgccttgcag gcggcgtcgc gctcaactcg aaagccaacg gaaagattgc
    23761 ggcatcgggc cttgtcgggc gaatgttcat ccagccggcg gctgccgatg acggtgcggc
    23821 gctcggtgcg gcgcttgctc cttatctgga cgacaacggc cgggtaccga accatgccat
    23881 gcgccatgcc tatctgggtg attcgttcga tgacacggct gtcgaaagcg cgttgaagac
    23941 ctacaagatt agttacacgc acgtggacga cccggcgaaa gccgcggcgg aactgcttgc
    24001 gcgagggaaa attctgggat ggtttcaagg ccgcatggag ttcggtccgc gcgctttggg
    24061 cagccgctcc atcctcgccg atccgcgcga cccggagatg aacgcgaagg tcaatcaagc
    24121 ggtgaaattc cgggaatggt ggcggccgtt tgctccttcg attctcgccg agaaagcttg
    24181 cgattacttg gaaagcgctg ccgactcgcc gttcatgatt ctgacggcgc aagtgcggcc
    24241 ggaaaaacga gccgtgattc ctgccgttac gcatgtggac ggcagcgcga ggccgcagac
    24301 tgtcgagaaa gaaaccaatc cgttgtactg gcggctgatc gatgaattcg gaaaacgcac
    24361 cggagtctac gtggtcctga acacatcgtt caacctgcgt ggggaagcca tcgtccacac
    24421 ccctaccgat gcaatccgca cgttcttcag ctccgggatg gatgcgctcg tcatcggatc
    24481 gttcctcgtc gagaaatgaa caaacagcgc attttcacgc ttctcggtcg gcgcgatgag
    24541 ccggccgacg gtgtggggaa tattgtgctt atctgcgcga gcacaaccgg gcggcccacc
    24601 tgaactattt ttcgtgggag gccatcgccg tgcgctacgc tgtcgtactg cgcggcgaac
    24661 attgaagaag ctcgttcggc gagtctggcg gaggaggagg gattcgaacc cccggggccc
    24721 tttcgagccc aacggttttc aagaccgccg gtttcaaccg ctcacccact cctccacttc
    24781 tgattgtacc tgcaatcgcg gtggttggcg ggctcagtga gcgcttgaat ctccggaatc
    24841 cacggaagtc ggcccagttt cgaagtcaat cccccagaaa tggggggagg gagtgaggaa
    24901 ttccacgccg acccgctggc ctttctcgac ctcactgccg aggtagacga cgcggcattc
    24961 ctgctcttcc tgtgtctccg ggttggcgag aaccagcaac tccccgttgc ggacttcgtg
    25021 cttcaggtag agcaggcctc cgtgaacgtt cacaaccact gtctcgcagt tctcgcggaa
    25081 cttccggcct tcctcgctac ggccctcagc ccgcatccgc atgcggtgga agacgcgcgg
    25141 gctgcgccgg ggagtggcga atgtgtccgg gctcatcagg ccatcttcgg atggatttcc
    25201 atcgttgtta attgtactcc cgcatgtttc ttcctgaaac acaaaccccc tctcacgctg
    25261 cttgtgcgcg gcgggtctga aatcatagaa tgcactgcgc ggcgacgggg ggcgcctttc
    25321 atggtccgtc cctgccttcg ctgcagaccg gtgcgcgcct gattccggcg ctcaatttca
    25381 gaccattcag aagggcttat cccagagggc tacatatgaa tcttgccgaa cacatcgaat
    25441 ctcggcgcga acagcatctt caggagctgt gtgaatttct gcgcatcccg agcatcagcg
    25501 cgaaaagcga gcacaagccg gacatcgagc gtgccgccaa gtgggtcgct gggaaccttc
    25561 gcggcgctgg atttaagagt gtggagattg ttcccactca acttcaccct ctggtgtacg
    25621 ccgagtccct ggaggctccc gggaagccca cgattctctt ctacgggcat tacgatgtgc
    25681 agcccgccga gccgttggag ctttggacca cgccggcgtt tgaacccact gtccgcgacg
    25741 gaaagctctt cggccgcggc acggcggacg acaagggcca ggttcacatt catctgaagg
    25801 cgttcgaagc gttgcaccgg atgaacggca gactgcccat caacgtgaaa gttttgatcg
    25861 aaggagaaga ggaagtcggc agcgtaagcc tctgggattt tgtccagaag aatcaggcca
    25921 agctgaaggc ggacgcgctc gttgtttccg acacttccat gctctcgcgc ggcgtgccct
    25981 cgatcaccta tgggctgcgc gggctgaatt actaccagat tgaaattacc ggaccggagc
    26041 gtgacctgca ctccggggtg tacggcggcg cggtgccgaa tccgctcacg attctcgccg
    26101 agctcttcgc ggagctgcac gacaagaatt tccgcgtcac cgtgccggga ttctacaatg
    26161 acgtggcgaa actctccgcc gccgagcgca aggccctgag cgcgctgccg tggagcgtga
    26221 aggaattcaa gaaagccgtc ggtgcgcctg cctattgcgg ggagaagggc ttttccattg
    26281 ttgagcagct atggacgcgc cccacgctcg agttgaatgg tgtctggggc ggctacctgg
    26341 gcgaaggcgc gaaaaccgtc attccctcga aggcctgggc aaaattttcc acgcggctcg
    26401 ttccccatca ggaaccggaa aaaatcgcca ggctggtcga aagacacctt cgcaggctgc
    26461 tgccaaaaac ggtgaagctg aatttcgaaa cgctcagcac gggcaaaccc tgggttgcgc
    26521 ctttccatgc gccgatcttt ctcaaagcgc aagccgcgct ggagaagggc ttcggcagaa
    26581 aggccgtttt catccgcgaa ggcggctcca ttcctttcgt aacgcagatg tacgacacat
    26641 tcaaagtgcc gtgcgttctg atgggcttcg gcctgccgga tgaaaacgcg catgcgccgg
    26701 acgagcacat cgcgctcgaa aactacttcg gcgggatcaa ggctatcgcc cacttctacg
    26761 aggacctcgc cacgctctga ggaccgggcg tttctcccgg cgggcgcgtc cgccggggac
    26821 accctcccaa aatccccgtt tttccggcct tttttgctgt attttccttg cgttcctcga
    26881 tttcgttggc tatagtgccg tccggcgcgc tttccgggcg taaagcagcc taaagatcgc
    26941 gtgccccacc ccgattcgtt cggggctgaa cgaacgaaaa ttcaggagga tagacaaact
    27001 cgatggcagc caagccgatg accaaaagca aagttgtggc gagcattgcc gagaagtgcg
    27061 aaacgtcgaa aaagaccgcg gccctgtttt ttgaagaact tttcaatctg gctgtgaggg
    27121 aatgcaaggg tgcggccggc aagttcgtga tcccgaacat tggccgcgcg gtaaaggcgc
    27181 accgcaaggc gcgcatgggc cgcaatccgg cgaccggcga ggcgatcaag atcaaggcca
    27241 agaccgttgt ccgcctgcgc gccgcaaagg cgttcaaaga cgccgttctg aagtagttcg
    27301 ttctcgcgga agcgagatac ccggccggac ccggcgcaag tccagcgggc gtcgagcggt
    27361 ggctggggtg tcagagttga gcttgtcctt tgtcggcgga gtcttcgcgc cgtcggcaat
    27421 gggctgcttt cctggcgatg gcctcggcgt ctcgctcgat gtgcaaccat ctaattctca
    27481 gtcgtttaca ctctgacttt catccccgaa gcaaacccgg tacgaaggac agttgtcccg
    27541 caggcacctg ccaataaccg ggtttttgac gtctgcctcc ttgcgtgtta ggaaagaagg
    27601 gatgagcacc cctgaatctc ttccgcgagc gtgggaactg ctccaggaag cttaccaggc
    27661 ccaaatggaa ggcgagtacg agcgcgcggt cgacctctat caggcttcgc tcaagttgca
    27721 ccccactgcc gaagcgcaca cttttctagg ctggacttac catttcctgg gacacctcga
    27781 ggacgccatc gccgagtgca agaaagccat cgaagtggac cccgatttcg gcaaccccta
    27841 caacgacatc ggagcgtact tgctcgagat ggggcgctac g
//
LOCUS       DASYVO010000300        34500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_189140, whole genome shotgun sequence.
ACCESSION   DASYVO010000300 DASYVO010000000
VERSION     DASYVO010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 34500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34500
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189140"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VGR03_10035"
     CDS             <1..329
                     /locus_tag="VGR03_10035"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEV1994659.1"
                     /translation="LRSRTGKRIAVIVSDTFGRAWRLGQVNVAIGAAGLPVLVDLRGS
                     CDLFGKRLHATILALADELAAAAGLLMGKADGLPVVVIRGLRCRAPNKSKAADIIRPA
                     SEDLFR"
     gene            333..1583
                     /gene="cofG"
                     /locus_tag="VGR03_10040"
     CDS             333..1583
                     /gene="cofG"
                     /locus_tag="VGR03_10040"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HEV1994660.1"
                     /translation="MMKTADDIGTRQAETRRGVEAALDRAHAGRGLGRDAAILLYQHA
                     ATVELLRAATDVRALGKGSTVSFSKKVFIPLTTLCRDYCSYCTFRKDPGQPGAHFMTP
                     DEVLALAETAREAGCKEALFSLGDQPERVFPEARDFLRRQGFTRTLDYVAAMSEMVLE
                     KTGLLPHANPGVMDRAALERLRESNASVGLMLENISPRLMRSSGPHANAPDKVPALRL
                     RTMEEAGRLSMAFTTGILIGIGETQEERVDSLLAIRAMHERHGHIQEVIVQNFRAKPD
                     IPMAAHPEPALEEMLRTVAIARLVLGGEMNLQAPPNLSYEEFPRLLEAGINDWGGISP
                     VTRDFINPEAAWPQIDRLRRETEARGFTLRERLAIYPEYLRREEFLAPAVRERGLRLA
                     DAGGYAREAAGPGPLAASLARGAT"
     gene            1580..2533
                     /gene="cofD"
                     /locus_tag="VGR03_10045"
     CDS             1580..2533
                     /gene="cofD"
                     /locus_tag="VGR03_10045"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEV1994661.1"
                     /translation="MSGKARQKQRSVRLAALAGGVGASKLLLGLYGECDPARLDIIVN
                     TGDDIVLHGLRISPDLDIVTYTLAGVVDRAKGWGFRGETFHALERLRCFGREAWFQLG
                     DRDMATHLHRTAQLAAGKSLTEVADDIRRAFGVKSRILPMTNQAVPTIIDSSEGPLHF
                     QEYLVKRRAEPIVRGIRFENVESAEPAPGVLEAIRAADRIWICPSNPLISIGPILAIR
                     GIREALQKRKRDVIAVCPIVGGKSLKGPSDRMLAQLDYEVSARGVARLYADFAGTFVV
                     DPADRGQVASIRSLGMNVAVVPTVMRTLAQKRKLALALLAL"
     gene            complement(2563..3240)
                     /gene="npdG"
                     /locus_tag="VGR03_10050"
     CDS             complement(2563..3240)
                     /gene="npdG"
                     /locus_tag="VGR03_10050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEV1994662.1"
                     /translation="MSRTIAILGGTGPEGSGLAYRWARAGERIVIGSREAARAVEAAT
                     RLTARIGPGAAVEGLENKAAASRGDIIVLTVPFSGHAALLKSLKNAWKTGAIVIDTTV
                     PLAATLGGAHTRMLGVWQGSAAEQARELVPAGIGVAAAFQNLSAELLAGDAPVDCDVL
                     VCSDDERAREAALDLAGKIPGARALNGGKLENARIVESLTALLIGLNVRYKAHGAGIR
                     FTGLLPK"
     gene            complement(3458..4480)
                     /locus_tag="VGR03_10055"
     CDS             complement(3458..4480)
                     /locus_tag="VGR03_10055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1994663.1"
                     /translation="MKTDSLTRYSLVVPFFNEQENIPPLYMKLTEVMDAIGEPYEMIF
                     IDDGSRDNTYKVLEDIFENDPRVNIVRLRRNFGQTAALKAGFDFARGEVIISMDGDQQ
                     HDPEEIPKFLEKIAEGYDLVSGWRVARRDPWLTRRIPSRVANWMMAKLSGIAVHDFGT
                     TFKAYRREIIQEIQLYGELHRFIPALAASTGARIAEVPIVNLQRQSGKSHYGIGRTIR
                     VFLDLLIVKFLLDYSTRPLQLFGVVGMAGLGSGMLICLSLAFKKLYFGTALFTQHGPL
                     LLLGIALIVSGLQFISMGLLGEMMARTYYESQNKPIYSLREVKSHRKELEDSAESTRP
                     SGNPER"
     gene            complement(4482..5360)
                     /locus_tag="VGR03_10060"
     CDS             complement(4482..5360)
                     /locus_tag="VGR03_10060"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEV1994664.1"
                     /translation="MRALIVNADDLGWTDGVNRGIAEAHRNGIVTSASLLANGAALAT
                     AVELARLETRLGVGVHLNLSDGPPMLPRWQVPSLVNGAGMFAGGPGSLLVRRLQGRLR
                     LEEVEREWEAQIQKVRDAGVQPTHLDGHKHVHMLPGLFELALRLAQRHGIRAIRIAHE
                     APRAAASLSNGAVSVSPLGAKQAVQAGALRLISMNAPALARRAGIAAADYFCGITQTG
                     LLRESGLASLLTNLPSGLTELMTHPGYAGAALLATPTRLQASRESELAILTAPAIRNL
                     VASLGIRLVNYGIAGR"
     gene            5554..5898
                     /locus_tag="VGR03_10065"
     CDS             5554..5898
                     /locus_tag="VGR03_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994665.1"
                     /translation="MGSHTRERMTGKGKYIGKRRGKRMNSRVPVRLEWDAEDGKRITI
                     EAHTRVVNSYGCMVVLPDSLELGHRMALINVAMGNSGVAVIVWKGEPRSDGWEYGIEL
                     VAPEMDFWGIEF"
     gene            5895..6266
                     /locus_tag="VGR03_10070"
     CDS             5895..6266
                     /locus_tag="VGR03_10070"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEV1994666.1"
                     /translation="MKHFKNKAAKAKEEPERAAALMEERRRSLRVVLRMPVLLHVPGR
                     AQALHGMTLAVSEVGAMIVIPEPLPTGTKVTIENPNSKKRVEASVTRAARLTPEGAVL
                     PVEFAEKAPAFWDVFFPPGGN"
     gene            complement(6313..7353)
                     /locus_tag="VGR03_10075"
     CDS             complement(6313..7353)
                     /locus_tag="VGR03_10075"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1994667.1"
                     /translation="MAFVGLILALGLIPAWGQANTKVSGKVLDQSGKPLAGVQVVYTS
                     LSNARSYKFRTDRNGEFSGVGFPVDNYQVEVFATDAQSLYKEKISVTANPASRSLLVD
                     LSKESPSRPTLSKEQIETVKKQTEKAKNMNVLIQQANDAVNARNWQQAIPLLQQLVAA
                     DPTRWEFYSALGDAQLNTGQYDRAVENYQKGIQAAESNTAVDPQKKAGIAKMLTNKGN
                     AYLKLHRNNEALAAYTKAAAMDPNPGTAYFNLCATQYNAGNMDDAAAACDKAIAADPT
                     KADAYFIKGSAMYGNGKMDAANKYVVPPGTIEALNKYLELAPDGSHAADVIAMLEALG
                     AKVETTYKQRKK"
     gene            complement(7431..8438)
                     /locus_tag="VGR03_10080"
     CDS             complement(7431..8438)
                     /locus_tag="VGR03_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEV1994668.1"
                     /translation="MTTAPASQDSTGQRPTLAEIAAAHARIAPHIHRTPVLTSASLDA
                     AAGARIFFKCENLQKTGSFKIRGATNAIFSLSDEEAARGILTHSSGNHAAAVACAARW
                     RGVPAWIVMPNNAPAVKCRAVEGYGGRITFCDPTVSARSEAAERIQKETGAVMVHPYD
                     DDRIIAGQATAVKELLEEIPDLDAVLAPVSGGGLLSGTALAAKAIRPAIRVFGCEPAL
                     ADDAFRSLATGTLHSLEHSDTIADGLRASLSPRTFAILRRHAAGILLVSEEEMIAAMR
                     TILERMKIVVEPSSAVAVAPLLKFPGAAGLQLPAREDGAPPKVGVILSGGNVDLSSLP
                     F"
     gene            8690..9055
                     /locus_tag="VGR03_10085"
     CDS             8690..9055
                     /locus_tag="VGR03_10085"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEV1994669.1"
                     /translation="MHNSDRRLFPRYTLRIPVRYRMADFSTDMAEHTTEAINVSRNGL
                     FLSTKAPLPLGATLDMALRVPIEISGIHTQEARCKGHIVHEQVIGDGRMGYGVQIERM
                     NSPYVRQDEHNRMVAAGTF"
     gene            complement(9086..9922)
                     /locus_tag="VGR03_10090"
     CDS             complement(9086..9922)
                     /locus_tag="VGR03_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994670.1"
                     /translation="MNARAFLQVLTRAALVVCGVFVVVVLLAAMPNHRAPALPAQEQQ
                     PPPGQQTSSKTSKDDMGAMHHDHMATKDDSDEKQSEGNAVHDMMRGHHHGNGPHMHMT
                     PPRPRTRADEERAERIAAELRSGIEKYKDYHAAFADGYKIFLPNIRLPEYHFTNYWNG
                     FLEAFTFDPARPTSLLYKHTAEGYELVGAMYTMPKNATEEQLNARVPLSVASWHLHTN
                     LCMPAHYDGARVDWTKFGLRGSISTEKACEAAGGRFRPSIFGWMIHVYPFEESPDKVF
                     AH"
     gene            10053..11363
                     /gene="aroA"
                     /locus_tag="VGR03_10095"
     CDS             10053..11363
                     /gene="aroA"
                     /locus_tag="VGR03_10095"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV1994671.1"
                     /translation="MGKEIISEARSITGGIEPPGDKSISHRYALLAALAEGTSELRHF
                     SSAADCQSTLECLGALGVKAKREKDSIRIAGKGLRGLQRPRRALDAGNSGTTMRLLAG
                     ILVGQKFSSKITGDATLRRRPMKRVVEPLRQMGAEIRAQEDNFAPLEIRGGELRAIDY
                     AMPVASAQVKSAVLLAGLFADGTTSITEPVQTRDHTELALEAFGTRVEKKGRCARVRG
                     MTATDGGGGLEAQALDIPGDLSSAVFFIAAALLLPQSKLLVHNVGLNPTRTAVLDLLV
                     SMGGAIRIVSPMVANGEIAGDLVVHSSELKGGIIEGEQVALLIDELPMLAALGPFTAE
                     GIEIRNARELRVKESDRIAALAENLRRMGARVEERPDGLRIDGRVAGRLHGTEIDPCG
                     DHRIAMAFAVAALAAEGATQIRDAECAAVSYPEFFRELRRLAGA"
     gene            complement(11370..12719)
                     /locus_tag="VGR03_10100"
     CDS             complement(11370..12719)
                     /locus_tag="VGR03_10100"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEV1994672.1"
                     /translation="MAADPTRRSSLFSALVLIFFGLFFLLHYYRGFQLGHIFTRWWPV
                     LLILWGASMLYERSSTRPEGAARRGFLGGREIALVFLILLLLVGVNITETIANRIRGH
                     IQPLGSTYPYDLEVASRTVAPDAHVTIRNTHGNIHVRPGSGPQIRLSGRKNVTAWTES
                     EARRLSSGSTVEIVQEGSGYEIRPAGSDTRESRIGFDLDVELPPQSSLTIRSNGGDIE
                     VSDLAGQVSIAAQSGDIEVRNVGDDVSVATRHGDVKVSGVKGSVKVSGTRGEVDVSNA
                     TGSLTLDGEFYGSIRAEKILKGVHFLSHRTDLTLTELGGRLEISPGSFEISNSTGNLT
                     ITTNSNDLKIENVSGRLKIDNRNANVQVRFASPPKEDVEITDSSASIVLTVPSNASFE
                     ITADTHSGDIDTEFQAPSLKKTVTQSGDSHLEGKLGARGPKIILKTSYGSISLHKGT"
     gene            complement(12726..12932)
                     /locus_tag="VGR03_10105"
     CDS             complement(12726..12932)
                     /locus_tag="VGR03_10105"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEV1994673.1"
                     /translation="MGPAILITIGVLFLLDRWRGEFFRFHYTWPVILLVIGFIKLAES
                     FASAEGHVGAFQPPQVPGSQPPGQ"
     gene            complement(12997..13626)
                     /locus_tag="VGR03_10110"
     CDS             complement(12997..13626)
                     /locus_tag="VGR03_10110"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEV1994674.1"
                     /translation="MNCAVHPKQEASGYCRHCGKAMCAACARAIHGVPYCEDCLAAVM
                     GHSAPGTSSAPGFAPQASAHSLPNPGLAFALGFCPGLGAVYNGEYTKAILHIVVFAAM
                     VAGLSNGNFGAGGIVALSLLLSGFVFYMAIDALRTARAKRAGAPSADPLQVWGSGKPV
                     GAFVLIGLGVFFLLVTFDIVSWDRMWDFWPVFLIAAGVVMLWNRLGRSS"
     gene            complement(13623..14303)
                     /locus_tag="VGR03_10115"
     CDS             complement(13623..14303)
                     /locus_tag="VGR03_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEV1994675.1"
                     /translation="MSWTCEQLEARLSDHLDGVLSPADREAFLAHSRSCARCAPLVAG
                     VSGLVTSLHSLEPVQAPPRLVYGILDKTLGPRESISGWRLALAWLRNLTSPKFAYGAL
                     SITATLGVLLAASGFSWHRPRMADLRPAAIYRNADRQAHLAYARSSKFFSDLRVVYEI
                     QSRLRPDAELSSEPDKPAPKSAPQKQPGVSNGPDSSPRQQNRANGLRPDLAVLAAALP
                     RLTPRSAR"
     gene            complement(14317..14886)
                     /locus_tag="VGR03_10120"
     CDS             complement(14317..14886)
                     /locus_tag="VGR03_10120"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1994676.1"
                     /translation="MEQDAQLVQQCLRGDGSAWEELVRRHTRRVFNLCYRFTGNGSEA
                     EDISQEVFLRVYRTLASYRSVHGGFATWMSSVTRNLLIDHYRRTKRDRLTDSLDDAMP
                     VVENKESSARRPDEQALLGELGAQIQAALKRLSPELREAVILRDLQGLEYMEIQTVLS
                     VPEGTVKSRINRGRIELARILQQMGVRPS"
     gene            15150..16202
                     /locus_tag="VGR03_10125"
     CDS             15150..16202
                     /locus_tag="VGR03_10125"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEV1994677.1"
                     /translation="MLKSRRRLIVIGIGVLLLGAILYHARHLLGPAELSGVELLRAIR
                     GARLSLLMASLAAIYVCYVLRALRWMSFSRHLGPVSFWNIYSMTLAGFSVLFLLGRAA
                     DPIRPLLLARKERQPVADTMGIYALERLFDTASTAVIAAIGLFLLPTHGGGDGVPNEL
                     ERAARTAGTILIAGVLAVAAVLVYLRLHGTSLLERRLEGWSTTRGWRSVAARIVLGFV
                     RGIQTIRSWGDLGSAVFYSAAHWGLIVVIYFWVTRSFGGRLGEISFSGATLVMAFTMV
                     GSTVQLPAVGGGSQVGSIVAYTAIFGVQREPAVAAAIVLWLITFAACSLAGVPLLIRE
                     GLSLGELRKMAVRKEK"
     gene            16242..16706
                     /gene="nrdR"
                     /locus_tag="VGR03_10130"
     CDS             16242..16706
                     /gene="nrdR"
                     /locus_tag="VGR03_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEV1994678.1"
                     /translation="MKCPFCARVDDKVIDSREGRAGDAIRRRRECLKCGRRFTTYERI
                     DEIPYMVIKKDGRRERFERPKILQGLLKACEKRPVATLKLEGIVEEIEGVVHEATERE
                     LTTTEIGEMIMHRLKKLDKVAYVRFASVYMDFKDVKEFMAELKGLLKDRSKK"
     gene            16738..18435
                     /locus_tag="VGR03_10135"
     CDS             16738..18435
                     /locus_tag="VGR03_10135"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEV1994679.1"
                     /translation="MNKMRARLSFYGLLSAFLLSGAAGYFAAYAAEESAGLPRAASTA
                     MRGIDAERIRATVKFLSDDSLQGRGTGQKGGDVAADWIAEQFRAYGLQPAGDNGSFFQ
                     DVKFYGVTTDQQKTQLAFVPKSGAQLTLKFADDYVATDQTHSGQSQIDAPLVYVGYGI
                     HAPEYQWDDYKGADLKGKVLLMLVNEPPSDDPNFFKGKALTYYGRWTYKFEEAARRGA
                     VGVILIHKTEMASYGWEVVRNSWGGESSILQDEKGAKLKSAGWIQLEVARRLAQAAGT
                     DLDTMMQNATSRNFKPVELPIQVKETIVSHVRSFSSRNVVGKVAGSDPKLRNQAILYT
                     AHYDHLGIHPNEPGDNIYNGAADNATGCGILLELARAFAGAKERPGRTILLAAVTAEE
                     QGLLGSKYLGEHPPIPARNISLDLNYDDVQPFGDPQQVVVSGAERTTIYPLVQEVAKD
                     FDLSIQPDSRPEAGHYYRSDHFSLARVGVPAFSVNEGALFKGHDLAWGEEKERDYVAH
                     RYHQPSDEYKPEMDFTGDAKMARFGFALGWKAATMPGLAGWQPGDEFERARRESQKP"
     gene            18514..18590
                     /locus_tag="VGR03_10140"
     tRNA            18514..18590
                     /locus_tag="VGR03_10140"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18548..18550,aa:Arg,seq:acg)
     gene            18599..18859
                     /locus_tag="VGR03_10145"
     CDS             18599..18859
                     /locus_tag="VGR03_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV1994680.1"
                     /translation="MFFVYILRSKSNQRHYTGHATDLSQRLGQHNSGITKSTKNRGPW
                     ELVHHEEFTTRAEAMRREKFLKSGQGREEVQRILRNARSSAG"
     gene            complement(18942..20585)
                     /locus_tag="VGR03_10150"
     CDS             complement(18942..20585)
                     /locus_tag="VGR03_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994681.1"
                     /translation="MKKISIVSLLLFLSAFPVAAQTTPPLRQQAAPSPARLDQEQPQK
                     EHKVTSEEARELFQSVDEILRFASADTLLPVKFPVKKAMVNRAQVEKYIGDKFKDDVD
                     RIRFERSELVLKKFGLLPRKFELHDFLIKLMGEQVAGYYDEKTKTINLLDWVELDMQK
                     AVMAHELTHALQDQSFDLGKMMKEEEEIEKRGPQDPNALIRIDEKSTARTAVMEGQAM
                     IVFVDYLLKPMGHSVEDSPKLLDMMRGELEQNAGGTLFDSAPLLLKEELVFPYGAGMR
                     FIRELLIAGGRKLAFSGVLERMPRTSREILEPQEYLAGRVVPPLLLPDFDFLKKDFEA
                     FDAGAVGELDVSILLKQYADNAVAARLSPEWRGGAYYAAGRKGAKPSDANSTAHIGLL
                     YVSKWSSDKVAQEFARIYAAALPQRYSKLEHERPEAAKPGREKYSSSDGPIFIELYND
                     LVIAVEGFDADTAEKLIQAARRQAGKDSKFAARAASETRSREMIFYGQASSNSPNWQI
                     LPPGAMLLNPTVLETFDQPDFSVYSNGTNPEWSFDQKLR"
     gene            20952..22205
                     /locus_tag="VGR03_10155"
     CDS             20952..22205
                     /locus_tag="VGR03_10155"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1994682.1"
                     /translation="MNESGKASNPVRFSAVASLVLSALLISLVGAQVAAAQGGDAITI
                     GVVLPITGREGKLGQYQREGIELAIKKINDGGGISVKGKKYIFKEVFYDDGSDSAKSA
                     SLTERAMSSDNVTAVIGGYSTALGEAESVMADRYQTPWITPGAAATSIFSHGYQYVFG
                     SLSPIAVLGSTTAEFLGSLVDQGKLKKGLKIALAVENTDHGIDYVNGIQDWIKMHPGY
                     FTVVFSEKFELGGTDFSGLLQKVKSVKADIFLSDAHLQDYITMQRQYLQSGMHHQMIS
                     YGARGPEADARKALGDGINYIFAAIWWSSKLSYPQVKQFITDYKQFTGHDPDSWYAVP
                     AYDSVRILAAAISQAGSTDKTKIRDALRNVSLKDSLLPGTVLKFGPNGQTGYPFVIVQ
                     NQPGGKVEIVYPRNGATAAPIAPTP"
     gene            22224..23117
                     /locus_tag="VGR03_10160"
     CDS             22224..23117
                     /locus_tag="VGR03_10160"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1994683.1"
                     /translation="MTAVDYLNLLIAALLLAGIYAAMSVGMTVIYGVMKIVNLAHAGF
                     LMLGAYFVYELFERFHIDPILGAVLALPVFFAFGMGVHWLLVRWLPKSDTPTLASLLL
                     MFGLWLVLQNVGYLVWGNMDRSIFTPRTLSVQRFGEITIPTLGLIVFAAAVISLAALQ
                     IVLNRTWFGRSIRALIQNPYAAQIVGVDDQKTARLTFGLGTAFAGFAGALLAMLFSFN
                     PDFGRQFLLRAFVIIVLGGLESVSGVALGALILALVETFSILKLPAGYQPAISFALLV
                     VVLVIMPKGIAGLLEKKWRVT"
     gene            23114..24121
                     /locus_tag="VGR03_10165"
     CDS             23114..24121
                     /locus_tag="VGR03_10165"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1994684.1"
                     /translation="MKISAALSRRSAVIFPCLLLIVLLALPAAGLSANMVRMLFLTAV
                     WTITSLSWNLLGGFAGQVSFGFAVFYGLGAYSTALLMHAGVNPYVSFLGAAATAALAS
                     VVIGLPTFRLRGPYFAIATIGVTEAVRVVMNNLDITGGASGYRVTEPGPFRPLEHYYT
                     AIAAVAFAVVVSVVISRSRFGLALRAIKQDEDAAADLGVNPYTCKLRIHAVAAALTGI
                     AGGIYARRAAFIHPGDVFAFQTGISILLMPVIGGIGTVWGAVLGGVTFGLVEQELVAN
                     FPQVHLLLYGSLLILIVLLEPNGLVGLLRRLFRRTREDSGSQDADKVFWGRGGLEGRK
                     L"
     gene            24069..24794
                     /locus_tag="VGR03_10170"
     CDS             24069..24794
                     /locus_tag="VGR03_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1994685.1"
                     /translation="MLTKYFGGVAALKDVSFEVREKHICGFIGPNGAGKTTLFNVITG
                     AVRPSAGMVHLRGSDVTGLRSATLVARGVARTHQIVRPFRALTVLENVQVAIHYGRRS
                     VRRTAAAREYAMDVLKLVGLEHKAQRQASVLALGEHKRLEVARALATAPDLLLLDEIC
                     GGLTTAETASMLDLLQRIREDGATIMYVEHDMKAVMSVCDHITVLNFGRKLAEGKPHE
                     IQNDPAVIEAYLGKATFGSAPRA"
     gene            24787..25521
                     /locus_tag="VGR03_10175"
     CDS             24787..25521
                     /locus_tag="VGR03_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1994686.1"
                     /translation="MPSGQDANLLVADSLEVRYGVVQVLWGISFSVARSKVTCIIGSN
                     GAGKTTTLNTIAGVLPVKSGRILLDGEDVTSLPPHQRVKRHINLVPEGRQLWPGMSVE
                     ENLLMGCFLPGLRGHAKAGLARVYDLFPPLKERRRQLAGTLSGGEQQMCAIGRGVMTE
                     PQLLMLDEPTLGLAPKLVDEIFELIGNIARQGVTILLVGQNVNYTLQVSHYGYVMESG
                     RIALEGPSEKLRNDDHVRRAYMGAHD"
     gene            complement(25538..26038)
                     /locus_tag="VGR03_10180"
     CDS             complement(25538..26038)
                     /locus_tag="VGR03_10180"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV1994687.1"
                     /translation="MNSLIKAADSAYAWFLKAANLLQSPLLLVIRLYWGWQFFQTGLG
                     KLSDIPKVIGYFTDLGVPAPAFNAYFIALLEVGGGILLVLGLGSRLIAVPLAIDMIVA
                     YVVADREALSSIFSDPGKFYAAAPFTFLCASLVVLFFGPGLFSLDTLIARFRAKRQKQ
                     SPVAAQ"
     gene            complement(26046..26933)
                     /locus_tag="VGR03_10185"
     CDS             complement(26046..26933)
                     /locus_tag="VGR03_10185"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEV1994688.1"
                     /translation="MDLEQLQRRVFDVIRQPLTASEGMRKRTRDGKPVKKIVEEMIKP
                     NDRLTSFERLEIYNRVYWFRILSALAEDFPGLRAIIGQRNFDKLLVAYLSDCPSESFS
                     LRNLGARLEEWLRDHPKFVPKTSRMALDMVRLEWADIEAFDGAELPKLAEGDLRNLGE
                     DPSFHVQPYLRLLDLAYPVDDLLLSVRQNEEQESDIASNVVLERTPRARGRRLSLPRP
                     KKVFLAVHRQDDVVYFKRLEREAFALIRALQQGKILSQAIETSVHWTNQPVERITERL
                     QGWFANWSSLGWFCKQPEN"
     gene            complement(26933..27847)
                     /locus_tag="VGR03_10190"
     CDS             complement(26933..27847)
                     /locus_tag="VGR03_10190"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEV1994689.1"
                     /translation="MPANRFNGHTEYGVGIGLRIPHYQHIFEKKPVVDWFEIISENFM
                     VDGGRPLRVLDQILDQYRVVQHGVSMYFGSAEPLNREHLRRLKTLVKRTNTPWLTDHL
                     CWGSVDGRYSHDLLPMPYTFEAAKKTAQKIREVRDFVEVPIAVENVSSYAEYHVSEMT
                     EWEFLIEVVEEADCGILLDVNNIYVSSQNHSFNPFDYLNSVPPARVAQIHVAGHSKFE
                     KYILDTHDHPVLDPVWQLYARAIELAGPTATLLEWDDRIPSFEEVHQEALKAKKFIKA
                     KQPVLANQRLGPKQRAASNPYVAEKIGA"
     gene            complement(27935..28210)
                     /locus_tag="VGR03_10195"
     CDS             complement(27935..28210)
                     /locus_tag="VGR03_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994690.1"
                     /translation="MTKSMRALLVGAALTGFVAGSTAAMARTGDEKAPKSDSGKKATK
                     KKKVEIHSCAGKNSCKGKGGCGTTKGKNDCKGKGGCSTDPKHPMPKS"
     gene            28892..30247
                     /locus_tag="VGR03_10200"
     CDS             28892..30247
                     /locus_tag="VGR03_10200"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1994691.1"
                     /translation="MAKRKNNGFTRRDFIKWSGSGAIALGAGSGPFFLFPGKAAAQQK
                     TLKICQWSHFVPAWDKWFNETYTKQWGEKHNTNVIVDNINLTELKTRAASEISAKKGH
                     DLFMLLSPPAAYEKQVIDMTHVYQEVEKKHGKKIDLAHKSTYNPKTKKYFAFSDAYTP
                     DPGNWHKDWWTEAGYPNGPDTYDDLLAGARKIRKSDKSGPPCGIGLGQELDTSMAMRA
                     ILWSFGGAEQDVEGNVTINSKNTIEALKFMKALYQDTETQEVFTWTPPSNNQAMLAGR
                     VSFVMNAISITRQSEREHLPIDSKIMVSQALKGPVRRIAAEHVMNCYVIWEFAENKEG
                     AQRFLIDYMDSFHDGFVAGQFYNFPCFPKTVPNLKQEIANDSRANPPDKYKVLGNVLE
                     WATNVGYPGYATAGIDEAFTTWVIPTMFANVARGQSTPEEAAKIAEAEYKRIFARNTG
                     A"
     gene            30247..31284
                     /locus_tag="VGR03_10205"
     CDS             30247..31284
                     /locus_tag="VGR03_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1994692.1"
                     /translation="MAVVETRSLTKIFKEGELGAVNDVNLVTREGEFLVFLGPSGSGK
                     TTLLRMIAGLETPTAGEILIGGQVVNHLTPRERRIAMVFQSYALYPHLTVYKNIAFPL
                     KAQNVPKKLHREKVEWAVSLLGISSLLQRKPRELSGGERQRVALARAIVREPSVFLLD
                     EPLSNLDAKLRLSAREELERFHRRIGTTTIYVTHDQVEAMAMGDRIIVLNKGEVRQFG
                     TPKDVYDEPADTFVATFLGSPPMNLVENGEVIVGFRPEHFRPAETVTEARFTFKYRVE
                     NVEYLGAEYILSGFLAGGRAEGKKAIARLLLGQSFEIGTTYEFAVPERHLNFFDRTSE
                     KRVAARALVWQ"
     gene            31329..32189
                     /locus_tag="VGR03_10210"
     CDS             31329..32189
                     /locus_tag="VGR03_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1994693.1"
                     /translation="MGAAMFGPAVLYIVLLIGLPFILSILYAFSDARIGSIGFHLVGL
                     ENFRSILQSPSFRTALKNSVIFTVCAQVIVVVCSTTLSIALEEKFRGRGLVRFLILLP
                     WVAPVSLGTIGWKWILDSIYSVITWLLIHFHFVGTYSAPMWLGEPHLAMTSVILVHCW
                     RLIPFSTVIMLAGRSAIPKEIPEAAAIDGAGFWRTLFQISIPMMAPIISVAVLFGTIF
                     TFTDMTVVYILTAGGPFDSTQTLPSLAFATGIQGSDLAAGAAISIFLVPVLVVIAYLM
                     LRVAHRAEVV"
     gene            32189..33040
                     /locus_tag="VGR03_10215"
     CDS             32189..33040
                     /locus_tag="VGR03_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1994694.1"
                     /translation="MKKATSRKLGPRVARWAVLSVFVVLLAFPFYWMFIAAFKQNIDL
                     YNVESNPFIFNLKPTLDHLRLLFNETRYVRWLGNTALVGVLVVAITLLLSVPAAYALA
                     RLTGRWGQRLGIGIFLTYLVPPTLLFIPLSRVVAILGLQDTIWSLVLVYPSITVPFSI
                     WLLMGFFKSIPKELEDAAMVDGLTRFGAFIKMVVPVSISGILTVVIFTFTLVTQEFVY
                     ALTFISPESQQMVGVGIPIFLVRGDVYFWGSLMAACLIASLPIAFFYNLFLDRFISGF
                     TVGAVKG"
     gene            33155..33736
                     /locus_tag="VGR03_10220"
     CDS             33155..33736
                     /locus_tag="VGR03_10220"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEV1994695.1"
                     /translation="MATSRRDFFKISAVGGVAAAVLGFDLAPAYAQLRALKIARATET
                     RSTCPYCAVSCGVIIYTIGDKAKNVTPQVLHVEGDPDHPINRGTLCPKGASLMQDIMN
                     TRRLIKPEVRRPGSDHWEEIPWNDAINEIGGWIKKTRDATFIEKDAKGNTVNRCESIA
                     FTGGCTDTNELNYLVVKTMRSLGLVHLENQARV"
     gene            33785..>34500
                     /locus_tag="VGR03_10225"
     CDS             33785..>34500
                     /locus_tag="VGR03_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV1994696.1"
                     /translation="MTNGWVDFRNSDMMLIMGGNPAENHPCGFKWALEAKRIRNAKII
                     SVDPRFTRTSAVADLYCHLRAGTDIAFLGGMIRYAIENKRIAHDYLVNYTNAAFIVKD
                     GFKLPEDGLFSGFDPATQVYDKSTWNYEEGGDMAGKEDHPAPAAKPVAAPAGGKPAPP
                     PALPPNVAYDLTLQHPRCVFQLLRKQYSRYTPEMVERITGAPREQFLKVADMFTSIRK
                     DGDTKKVATVIYALGWTQHS"
BASE COUNT         6799 a         9919 c        10909 g         6873 t
ORIGIN      
        1 gcctcagaag ccggacgggc aagcgtattg cggtcatcgt cagcgatacg tttggccgcg
       61 cctggcggct cggccaggta aacgtcgcga tcggagcggc aggcctgccg gtcctggtgg
      121 acctgcgcgg ttcgtgcgac cttttcggga agcgtctcca tgctacgatt cttgcgctcg
      181 cggacgaatt ggcggccgcg gcgggtctgc tgatgggcaa ggcggatggc ttgccggtgg
      241 tggttatccg cggcttgcga tgtcgcgccc cgaacaaatc caaggccgcg gatatcatcc
      301 gtccggcttc ggaagacctc ttccgctaga acatgatgaa gaccgctgac gatatcggca
      361 caagacaggc ggagacgcgg cggggcgtcg aagcagccct cgaccgggcg cacgcggggc
      421 gcggtctggg acgcgacgcg gcgatccttc tctatcaaca cgcagcgacg gtggagcttt
      481 tgcgcgccgc gactgatgtg cgggcgctag gcaaggggag cacggtcagt ttttcgaaaa
      541 aggtattcat tccgttgacg acgctctgcc gcgactattg cagctactgc acgtttcgga
      601 aggatccggg ccagcccggc gcgcatttca tgacgcccga cgaggtgctg gcattggcgg
      661 aaacggcgcg ggaggccggg tgcaaagagg cgctgttcag cctgggcgac cagccggagc
      721 gtgttttccc ggaggcccgg gattttctcc gcaggcaggg gtttacgcgg acgctcgact
      781 acgtggctgc aatgagcgag atggtcctcg agaagaccgg cctgctgccg catgcgaacc
      841 ccggcgtgat ggaccgggcc gcgctggaac gattgcgcga atctaacgcc agcgtgggcc
      901 tgatgctcga aaacatcagc ccgcgcctca tgcgtagctc cgggccgcac gcgaacgctc
      961 cggacaaggt tccggcgctg cgcctgcgca cgatggaaga agcgggacgc ctttcgatgg
     1021 ccttcacgac gggaatcctt atcgggatcg gcgagacgca ggaagagcgc gtcgattcac
     1081 tgctggccat ccgcgcgatg catgaacgcc acggccacat ccaggaagtg atcgtgcaga
     1141 atttccgggc caagccggat atacccatgg ctgcgcatcc ggagccggcg ctcgaggaga
     1201 tgttgcgcac ggtggccatc gcacggctcg tgctcggcgg ggagatgaat ctgcaggctc
     1261 cgccgaatct cagctacgag gagtttcctc ggttgctcga agcggggatc aacgactggg
     1321 gcggaatctc tccggtgacc cgcgatttca tcaatccgga ggcggcctgg ccgcagatcg
     1381 atcgactgcg ccgggagacc gaggcgcgcg gattcacgtt gcgcgaacga ttggcgatct
     1441 accccgagta tctgcgacgg gaggagttcc tagcaccggc ggtgagggag cgcgggctgc
     1501 gccttgccga tgcaggaggc tacgcgaggg aggcggccgg cccggggccg ctcgccgcgt
     1561 ctcttgcgcg gggcgccacg tgagcgggaa agcccgccag aaacagcgat cggtccgtct
     1621 ggccgccctc gctggtgggg tgggcgcttc gaagctcctc ctcggtttat atggggagtg
     1681 cgatccagca cggctcgaca tcatcgtcaa cacgggcgac gacatcgtcc tccacgggct
     1741 gcgcatttcg cccgatctcg acatcgtgac ctataccctt gccggggtcg tggatagagc
     1801 gaagggctgg ggtttccgcg gggagacttt tcatgccctc gagcggctcc gatgctttgg
     1861 acgcgaagcg tggttccaac tcggcgaccg cgatatggcc acgcatctgc atcgcacggc
     1921 gcagcttgcc gcaggaaaat ccctcactga ggtggccgac gatatccgca gagccttcgg
     1981 cgtgaagtcg cggattctgc cgatgaccaa tcaagccgta cccacgatca tcgattcgag
     2041 cgaaggtccg ctgcactttc aggagtatct cgtcaagcgc cgggcagaac ccatcgtgcg
     2101 cggcatccga ttcgaaaacg tggaatcggc cgagcccgcc ccgggcgtgc tcgaggccat
     2161 ccgcgccgcc gaccggatat ggatctgccc gagcaatcca ctgatcagca tcgggcccat
     2221 cttggcgatc cggggaatcc gcgaggcgct gcagaaaagg aaacgagacg tgatcgccgt
     2281 ctgcccgatc gtcggcggca aatccctcaa ggggccgtcg gaccgcatgc ttgcgcagct
     2341 cgactatgag gtttcggcgc gcggtgtagc ccgtctctac gcggattttg cggggacgtt
     2401 cgtcgtggac ccggccgacc gggggcaagt cgcttccatt cgatcgttgg gaatgaatgt
     2461 cgcggttgtc ccgacggtga tgcgcacgct ggcacagaag cgcaaacttg ccctggcgct
     2521 cctcgccttg tgaccaggct agagcaattc aaatcgatgg ggctattttg gaagcaatcc
     2581 agtaaatcga atgcccgcgc cgtgggcctt gtaacggacg ttgaggccga tcagcagggc
     2641 agtgagtgat tcgacgatgc gggcattctc gagcttgccg ccgttgagag cccgcgcgcc
     2701 ggggattttg cctgcgagat cgagagcagc ttcacgggct cgctcgtcgt cgctgcacac
     2761 gaggacatcg caatccaccg gcgcatcgcc ggccagcaac tccgcgctga gattctggaa
     2821 cgccgccgcg acgccgatgc ctgccgggac cagctctctc gcctgctctg ccgcggagcc
     2881 ttgccacacg cccagcatcc tcgtgtgggc tccgccgagc gttgcggcga gtggaaccgt
     2941 cgtatcaatc acgatggctc cggttttcca ggcgttcttc agcgatttca gcagcgccgc
     3001 gtggccggag aagggaaccg tcagcacgat aatgtcgccg cggctcgcgg cggctttatt
     3061 ttccagccct tctacagccg cgccggggcc gatgcgcgct gtgagccggg tggcggcctc
     3121 cacggcgcgc gccgcttcgc gtgatccgat gacgatgcgc tcccccgcgc gtgcccagcg
     3181 ataggcaagc ccggaacctt ccggcccggt gccgccgaga atggcgatgg tgcgtgacat
     3241 ggacgctcgc ttgcgcatct ccgtgccggc catcggatcg tgagcccggc acggcaagag
     3301 tgggcgatta tacgcgaaga ggacgggagt tcacgggggg aagtatcggc cagcccttag
     3361 ggatgaccgc cccgccggcg cggctacagg ctaacgcaat tgttctgcgg agggaatggc
     3421 tgctcgagcg gcggagcgat tggaacggga agacgaatca acgctcggga ttgccggaag
     3481 gccgcgtgga ttcggcggaa tcctcaagct ccttgcgatg gctcttcact tcgcgcagcg
     3541 agtaaatcgg cttgttctgc gattcgtagt acgtgcgcgc catcatctcg ccgagcagac
     3601 ccatggagat gaactggagc ccgctgacga tcagcgcgat gcccagcagc agcagcggcc
     3661 catgctgcgt gaagagcgcc gtgccgaagt agagtttctt gaacgcaagg gagagacaaa
     3721 tcagcatccc cgaacccagg ccggccatgc ccacaacgcc gaacagctgc agcggccgcg
     3781 tcgagtagtc cagaagaaac ttcacaatca gcaggtcgag gaacacacga atcgtgcggc
     3841 cgatcccata atgagatttt ccgctctgcc gctggagatt gacgatgggc acttcggcga
     3901 tgcgcgcgcc cgtggaggcc gccaacgctg gaatgaagcg gtgcagttcg ccgtagagct
     3961 ggatttcctg gatgatctcg cggcggtaag ccttgaacgt tgtcccgaaa tcgtgtaccg
     4021 cgatgccgga gagcttcgcc atcatccagt ttgcgacgcg gcttggaatt ctccgcgtga
     4081 gccagggatc gcgccgcgcc acgcgccagc cgctcaccag gtcgtagccc tcggcgatct
     4141 tctcgaggaa tttcgggatc tcctcgggat cgtgctgctg gtcgccgtcc atcgagatga
     4201 tcacttcgcc gcgggcgaag tcgaatccgg ccttcagcgc ggccgtctgg ccaaaatttc
     4261 tccgcaggcg cacgatgttg acgcgcgggt cgttttcgaa gatgtcctca agaaccttgt
     4321 aagtattgtc gcggctgccg tcgtcgatga aaatcatttc ataaggctcg ccgatggcgt
     4381 ccatcacttc cgtcagcttc atatagaggg gcggaatgtt ttcctgttcg ttgaaaaacg
     4441 gcacaacgag cgagtagcgc gtaagcgagt cggttttcat gctatcggcc tgcaatgccg
     4501 tagtttacca agcgaatgcc gaggctggcg acaagatttc gaatcgccgg tgccgtcagg
     4561 atggccagct cgctttcgcg cgacgcctga aggcgcgtgg gcgtggcgag cagcgccgca
     4621 ccggcatagc cgggatgcgt catcaattcg gtgagcccgc tcgggaggtt cgtcagcagg
     4681 gaagcgaggc cggattcgcg cagcaggccg gtctgcgtaa tgccgcaaaa ataatctgcc
     4741 gccgcgatgc cggcgcggcg cgccagggct ggcgcattca tggaaatcag ccgcagcgcg
     4801 cccgcctgga ccgcctgctt cgcgccgagc ggtgaaacag aaacggcccc attcgaaaga
     4861 gacgcggcag cccgcggtgc ttcgtgcgcg atgcggatgg cgcgaattcc gtgccgctgc
     4921 gccagccgta gagccagctc gaacagcccg gggagcatgt gcacgtgctt gtgcccgtcg
     4981 aggtgcgtgg gctgaacacc cgcatcgcgc accttctgaa tctgcgcttc ccattcccgc
     5041 tccacttcct caagcctcag ccgcccttgc agccgtcgaa cgagcaggga gcccggcccg
     5101 ccggcaaaca tgcctgcgcc gttgaccaga ctcgggacct gccaccgtgg caacatgggc
     5161 gggccgtcgc tgaggttgag gtggacgccg acgcccaggc gcgtttccag ccgcgcgagt
     5221 tccacggcgg tggccagtgc ggcgccgttg gcgaggagag atgcgctggt gacgattccg
     5281 ttgcggtgcg cctcggcgat gccgcggttc acgccgtcgg tccagccgag gtcgtccgcg
     5341 ttgacgatta gtgctctcaa gagttgaggg aagcgatgcc cattcctgcc cggacgtcct
     5401 tcgagaatga cgctccgcgc ctttctccag gcgatttcag aaacttctcc gccttgtcct
     5461 tcgccggcct ttccggaaga cagctaatcc ttacgcgccg ggactattga cgccttcggc
     5521 ccccaactct aacatgaccg ttaaggggcg tgaatggggt cacatacacg cgaacgaatg
     5581 acagggaagg ggaagtacat cggaaagcgc cgcggcaagc gcatgaactc gcgcgtaccg
     5641 gtgcgcctgg aatgggacgc ggaagacggc aagcgcatca ctatcgaagc ccacacgcgc
     5701 gtcgtcaata gttacggctg catggtggtg ctgccggatt ccctcgaatt ggggcatcgc
     5761 atggcgctca tcaacgtcgc catgggaaat tccggcgtag cggtgattgt ctggaagggc
     5821 gaaccgcggt ccgatggctg ggagtacggc atcgagttgg tcgcaccgga gatggacttc
     5881 tgggggatcg agttttgaag catttcaaga acaaggccgc caaggcaaag gaagagccgg
     5941 agcgcgcggc cgcgctgatg gaagaacgcc ggcgcagcct acgcgtggtg ctgcgcatgc
     6001 cggtgcttct ccacgtgccc gggcgagcgc aggcgttgca tggaatgacg ctggccgtca
     6061 gtgaggtcgg ggccatgatc gtcattccgg agccgctccc caccggcacg aaggtgacta
     6121 tcgagaatcc caactccaag aagcgcgtcg aggcgagcgt cacccgcgct gcccgactga
     6181 cgccggaagg cgcggttcta cccgtcgaat tcgccgaaaa ggccccggcc ttctgggatg
     6241 tctttttccc gcccggagga aactgacgag ccggctcgga cggcagccgc gccgaatcct
     6301 tgaagcgttg agttatttct tccgttgttt gtatgtggtc tcgactttag cgccgagggc
     6361 ctccagcatg gctatgacat cggcggcatg agagccatcg ggcgcaagtt ccaggtattt
     6421 gttcagggct tcgattgtcc ccggtggcac gacgtattta ttagcggcgt ccatcttccc
     6481 attcccgtac atggctgaac ctttgatgaa gtaggcatcc gccttggtgg ggtcggcggc
     6541 gatagccttg tcgcaggcgg cagcggcgtc gtccatgttc ccggcgttgt actgggtggc
     6601 gcacagattg aaatatgccg tccccggatt aggatccatt gcggctgcct tggtgtaagc
     6661 ggcaagagct tcgttgttcc tgtgaagctt gaggtaggca tttcccttgt tggtcagcat
     6721 cttggctatc ccggcctttt tctgtggatc caccgcagtg ttgctctcgg ccgcctgaat
     6781 gcctttctga tagttttcaa ctgcgcgatc atactgaccg gtattgagtt gggcatcgcc
     6841 caaggccgag tagaactccc accgggtggg gtcggctgca accaactgct gcaagagcgg
     6901 tatagcttgc tgccagttcc tggcgttcac agcatcgttg gcctgctgga tcagcacatt
     6961 catgtttttg gccttctcgg tttgcttctt gacggtctcg atctgttctt tggacagcgt
     7021 ggggcgggag gggctttcct tggagagatc caccaggagg gagcggctcg cgggatttgc
     7081 ggtcacgctt attttctctt tgtataagct ctgagcatcc gttgcaaaca cttccacttg
     7141 ataattgtca accgggaacc ctaccccgga aaactcaccg ttgcgatcgg tccggaattt
     7201 gtagctccga gcattgctca gactggtgta aaccacttgg accccggcaa ggggtttgcc
     7261 gctttgatcc aggactttcc cgctgacttt cgtattcgcc tggccccatg ccgggattaa
     7321 gccgagggca agtatcagcc ccacaaacgc cacgagaacg gatttcctct tcattgcttt
     7381 tgactctcct tgatttccta gatgcggccg ccccgccacg cgcgtcgatc tcaaaacggc
     7441 agggaagata ggtccacgtt tccgccggac aagatcacgc ccaccttcgg cggggctccg
     7501 tcctcgcggg cgggaagctg caggccagcg gcgcccggaa acttcagcag cggagccacg
     7561 gccacggcgg aagatggctc cacaacaatt ttcatgcgtt ccaagatggt acgcatggcc
     7621 gcaatcatct cttcctcgct caccagaaga attcctgcgg cgtgccggcg gagaatggcg
     7681 aacgtgcgcg gagataggga agcgcgcagg ccgtcggcga tcgtgtcgct gtgctcaagc
     7741 gagtgaagcg tgccggtggc gagcgaacgg aacgcgtcgt cggcgagcgc aggctcgcag
     7801 ccgaagacgc ggatcgccgg acgaatcgcc ttcgcggcga gcgccgtgcc actcagcagc
     7861 ccgcctccgc tgaccggggc cagaacggcg tcgaggtcgg gaatttcttc gagcagctcc
     7921 ttcaccgctg tcgcctggcc ggcgatgatg cggtcatcgt catacggatg gaccataacg
     7981 gcgccggtct ccttctggat gcgctctgca gcttcgctgc gcgccgatac cgtgggatca
     8041 cagaacgtga tcctgccgcc gtaaccctca acggcacggc actttacggc tggcgcgtta
     8101 tttggcatca cgatccaggc tggcacaccg cgccaccgcg ccgcacaggc caccgcggcg
     8161 gcgtggttgc cgctggaatg cgtcaagatg ccgcgggccg cctcttcgtc gctgagggag
     8221 aagatggcat tcgtcgcccc gcggatcttg aacgagccgg tcttttgcag gttctcgcac
     8281 ttgaagaaga tgcgtgcgcc ggccgcggcg tcgagcgaag cagaagtgag caccggagta
     8341 cggtggatgt gcggggcgat acgtgcgtgc gccgcggcga tttccgccag ggtgggccgt
     8401 tggccggtgg aatcctgaga agcaggggcg gtggtcatga tctcgtgtga cggatgttcg
     8461 atttcaccgc tgctccatta tggcacagcg cggagaatcg cggagctgag ctggatgcat
     8521 ttcgggaatg gcgatgccgc caatgctaaa cgagattggg agagcacgat gccctccaca
     8581 aaaatggtac gaaaatggta cttgaccgaa acgttgaaac tgttaggcta gggttcccct
     8641 ccgaaaatca aaccagccgc gtcgcctgtc ccgcgcgtag gagcacaaaa tgcataattc
     8701 cgacaggcgt ctttttcctc gctacacgct tcgcatcccg gttcgctacc gtatggccga
     8761 tttttccacc gacatggccg aacacacgac ggaggccatc aacgtctcgc gtaacgggct
     8821 gttcttgtca acgaaagcgc ctctgcctct cggcgcgacg ctcgacatgg ctttgcgcgt
     8881 gccgatcgag atctccggaa tacacacgca agaagcgcgc tgcaaaggac acatcgtgca
     8941 tgaacaggtc atcggggacg ggcggatggg ttatggcgtg cagatcgagc ggatgaactc
     9001 cccgtatgtt cgccaggacg agcacaaccg catggtggct gccggaacgt tctagggttt
     9061 cgtcggcggc acagggatcg cttggtcagt gggcgaagac tttgtccggg ctttcttcga
     9121 aggggtagac gtggatcatc cagccgaaga tggagggccg gaagcggccg ccggcagctt
     9181 cacaggcctt ctcggtggag atggagcctc gcaagccgaa cttggtccaa tcgactctcg
     9241 caccgtcata atgcgcgggc atgcatagat tggtgtgcaa gtgccagctc gcgacgctta
     9301 gcggcacgcg cgcattcaac tgctcttcgg tggcgttttt cggcatggtg tacatggcgc
     9361 cgacaagttc atatccttcc gcggtgtgct tgtagagcag agaggtcggg cgcgccggat
     9421 caaaggtgaa ggcttcgagg aatccattcc agtagttggt gaagtgatat tcgggcaggc
     9481 gaatgttcgg aagaaaaatc ttataaccgt cggcaaaggc ggcgtggtag tccttgtact
     9541 tctcgatgcc ggagcgaagc tcggcggcaa tccgttctgc acgctcttcg tcggccctgg
     9601 tgcgcgggcg gggaggggtc atatgcatgt gcgggccatt gccgtgatga tggccgcgca
     9661 tcatgtcgtg aacagcattg ccttcgctct gcttttcgtc ggaatcgtcc ttcgtggcca
     9721 tgtggtcgtg gtgcatggcg cccatgtcgt ctttagaggt ttttgaagag gtctgctgcc
     9781 caggcggcgg ttgctgttcc tgggcgggca gggcaggcgc gcgatggttg ggcatggccg
     9841 cgagcagaac cacaacaaca aatacaccgc agacgacgag ggcggcccgg gttaaaactt
     9901 gcaaaaaagc gcgcgcgttc atgactcctc ctgtccccgt ctaggtatgt tacgcggacg
     9961 gcggaggaaa agtatcagcc ccacgcgcga gagatgcgga acggcttacc tgtacgaggg
    10021 gacaggaaag aagcccgtga tagactcacg gcgtggggaa agaaatcata tccgaggcga
    10081 ggagcattac cgggggaatc gagccgcccg gagataaatc catctcgcac cggtacgcac
    10141 tgctggcggc gctggcggaa ggaacgagcg agctgcgcca tttttcttcg gcggcggatt
    10201 gccagagcac gcttgagtgc ctgggagcgc ttggcgtgaa agccaagcgc gagaaggact
    10261 cgatacgcat cgcggggaag ggattgcgcg gactgcagag gccgcgccgc gcactggatg
    10321 cggggaattc cggaacgacg atgcggctgc tggcgggaat cctcgtggga cagaagttct
    10381 cctcaaagat cacgggcgat gcgacgttgc ggcggaggcc gatgaagcgc gtcgtggagc
    10441 cgctgcgcca aatgggcgcg gagatccgcg cgcaggaaga caacttcgcg ccgctggaaa
    10501 tccgcggagg cgagttgcgg gcgattgact acgcgatgcc ggtggcgagc gcgcaggtga
    10561 agtcggcggt gctgctggca ggcttgtttg cggatggtac gacttccatt acggagccgg
    10621 tacaaacccg ggatcacacg gagctggcac tggaggcatt cgggactcgc gtggagaaga
    10681 agggacggtg cgcgcgtgtg cggggaatga cagccacgga cgggggcggg gggctcgagg
    10741 cccaggcgct ggacatcccg ggggatcttt cgtcggcggt cttcttcatc gcggcggcac
    10801 tgctgctgcc gcagtcgaaa ctgctggtgc acaacgtcgg cctgaatccg acgcggacgg
    10861 ccgtgctgga cttgctggtt tcgatgggag gagcgatccg gatcgtttcg ccgatggtcg
    10921 cgaacgggga gatcgcaggg gacctcgtcg tgcacagctc agagttgaag ggaggaatta
    10981 tcgaggggga gcaggtggcc ctgctgattg atgaactacc gatgctggcc gcgctggggc
    11041 cgttcaccgc ggagggaatc gagatccgga acgcgaggga gttgcgcgtg aaagagagcg
    11101 accgcatcgc ggcgctggcg gagaacctgc ggcggatggg ggcgcgagtg gaagagcggc
    11161 ccgacgggct gcgcatagat gggcgagtcg cgggccggtt gcacggaacg gagatcgatc
    11221 cgtgcgggga tcaccggatc gcgatggcgt tcgcggtggc agccctcgcg gcggagggcg
    11281 cgacgcaaat ccgggacgcg gagtgcgcag cggtttctta tccagagttc ttccgggagc
    11341 tcaggcggct cgcgggggcg taatccgcgt tatgtgcctt tgtggaggga gatggagccg
    11401 tagctggtct tgagaatgat cttgggcccg cgcgcaccta gttttccctc aagatgggaa
    11461 tcgccggatt gcgtaacggt tttcttcagc gaaggagcct ggaattccgt gtcgatgtct
    11521 cccgaatggg tgtcggctgt gatttcaaaa ctggcgttcg acggcacggt gaggacgatg
    11581 ctggccgaag aatcggtgat ctcaacgtcc tccttcggcg gagaagcgaa acgcacctga
    11641 acgttggcat tgcgattatc gattttcagc ctgcccgaaa cattctcgat tttgaggtcg
    11701 ttggagttgg tggtgatggt caggttgccg gtggaattcg atatttcgaa actgccaggg
    11761 ctgatttcca gtcggccgcc cagctccgtg agagtgagat cggtgcgatg cgagaggaag
    11821 tgcacacctt tcaggatctt ctcggctcgt atggagccgt agaattcgcc gtcgagagtc
    11881 agactgcctg tggcattgct aacatcgacc tcgccgcgcg tgccggagac cttgacgctg
    11941 cccttgacgc cggagacttt cacatcaccg tggcgcgttg cgacgctgac gtcgtcaccc
    12001 acgttgcgga cttcgatgtc gccggattgc gcggcgatgg agacctgccc ggcaaggtct
    12061 gaaacttcga tatcgcctcc attggagcgg atcgtgagag aggactgcgg cggcagctcg
    12121 acgtcgagat caaatccgat gcggctctcg cgcgtatcgg acccggcggg gcgaatttcg
    12181 tagccgctcc cttcctggac aatctcgaca gtgctcccgg aggagagccg ccgagcttcg
    12241 gattcggtcc aagcggtgac gttctttctt ccggagagac gaatctgcgg gccgctacca
    12301 ggacggacgt gaatgttgcc gtgggtgttg cgaatcgtga cgtgagcgtc cggagcgacg
    12361 gtccgcgacg cgacttccag gtcgtaggga taggtactgc cgaggggctg gatatgaccg
    12421 cgaatcctgt tcgcgatcgt ttccgtgatg tttacgccga cgaggaggag gagtattagg
    12481 aagacaaggg cgatttcgcg gccgccgagg aagccgcggc gggcggcgcc ttcgggccga
    12541 gttgaggaac gctcgtaaag catactggcg ccccagagaa tcaggaggac aggccaccag
    12601 cgcgtgaaga tgtggcccaa ctggaaaccc cggtagtaat ggagaaggaa gaaaagaccg
    12661 aagaagatca gcacgagtgc gctgaagagc gaactgcggc gtgtgggatc ggcggccatg
    12721 agctgttact gacccggcgg ttgcgagccg ggaacctgcg gcggctggaa tgctccgacg
    12781 tggccttcgg cggaggcgaa agattcggcg agcttgatga agccgatgac aagaagtatg
    12841 acgggccagg tatagtggaa gcggaagaac tcgccgcgcc agcggtcaag caggaaaagc
    12901 acgccgatgg tgatcaaaat ggcggggccc attaaaccgc ggcaggcgca ccgccggcat
    12961 gcacagcgga ttcgattcac cattccctca ctcctcctag gaagaccgtc ctagacggtt
    13021 ccagagcatc acaacaccgg ccgcaatcaa gaacacgggc cagaagtccc acatacggtc
    13081 ccaagaaacg atgtcgaacg taacgagcag gaaaaagacg ccaagaccga tgaggacaaa
    13141 agcaccgacc ggctttccac tgccccacac ttgcagcggg tcggccgaag gcgcgccggc
    13201 acgcttggcc ctggcggtgc gcagggcgtc aatggccatg tagaacacga atcccgacag
    13261 taatagagat agcgcgacga tgcctccggc gccgaaattg ccgttgctga ggccggcgac
    13321 catggcggca aaaacaacga tgtgcaggat tgccttggtg tactcgccgt tataaaccgc
    13381 accaagtccc gggcagaagc cgagtgcaaa cgcgaggccg ggattgggca aagaatgtgc
    13441 agaggcctga ggagcaaaac cgggcgcaga ggaagtgccc ggcgccgaat ggcccatgac
    13501 cgcggcaagg cagtcctcac aatagggtac gccgtggatg gcccgcgcgc aggcggcgca
    13561 catggccttg ccacaatggc ggcagtagcc ggaggcttcc tgcttgggat ggacggcaca
    13621 gttcatcgag cgctccttgg tgtgagacgc ggaagggcgg ccgcgagtac ggccaagtcc
    13681 gggcgaaggc cgttggcgcg gttctgctgg cggggcgagg agtccgggcc atttgagacc
    13741 ccaggctgct tttggggagc gcttttcgga gctggcttgt caggttcgga agagagttca
    13801 gcgtccgggc gcagacggga ttgaatctca tagacgacac gcaagtcgct gaagaatttc
    13861 gagctgcggg cgtaggcgag atgggcttgg cggtcggcat tgcgatagat ggcagcaggg
    13921 cggagatcag ccatgcgggg ccggtgccag gaaaagcccg aggcggcaag aagaacaccc
    13981 agagtggccg tgatggaaag cgcgccgtac gcaaacttcg gggaagtcag gttgcggagc
    14041 caggcaagcg cgagccgcca tccagaaatg gattcgcgag gaccgagcgt cttgtcaagg
    14101 atgccgtaga cgaggcgcgg tggcgcttgg acgggctcga gcgaatggag gctggtgaca
    14161 agaccggaaa caccggcgac gagcggagca caccgggcgc agctccggga atgcgcgagg
    14221 aaggcttcgc gatcggcggg agaaagaacc ccgtccaggt gatcgctcaa ccgagcttcg
    14281 agttgttcgc aagtccagct catggctcgc tccgcgctaa gaagggcgca ctcccatctg
    14341 ctggagaatg cgagccagtt cgattcggcc gcggttgatt ctcgatttta ccgttccttc
    14401 gggtacgctg agcaccgtct gaatctccat atattccaga ccctgcaagt cgcggaggat
    14461 gaccgcttcg cgcaactcgg gagaaagccg cttcagggcc gcctggattt gcgcgccgag
    14521 ttcgccgagg agggcctgct catcggggcg gcgcgcggag gactctttgt tttcgaccac
    14581 gggcatggcg tcgtcgagcg agtcggtcag gcggtcacgt tttgtgcggc gatagtggtc
    14641 aatgaggaga ttccgcgtga cgctgctcat ccaggtggcg aagccgccat gcacggagcg
    14701 gtaactggca agcgtgcggt agaccctcaa gaaaacctcc tgcgaaatat cctcggcctc
    14761 gctcccatta ccggtgaagc gatagcagag gttgaacacg cgccgcgtat gacggcgtac
    14821 aagctcctcc caggccgagc cgtcgccccg gaggcattgc tggaccagtt gggcatcctg
    14881 ctccaacgtt gcatcagcct cgaatgccgc atcgtttctc ggcctgccaa taatggaacg
    14941 taaactaccc tcggaaagtt ccccggaggg cgaccgaaaa ccggtccatt ctagcgcgga
    15001 tgatacctgc cgcggaggcg agctacaagg acggaggcag gccgccggtt cggagccact
    15061 cctgcatcac gatggagcgt atccggggcc accagaaagg aatggcggag acttgaccta
    15121 gccggaaggg gccagtaggc tggcggccga tgctcaagag caggcgcaga ctcatcgtca
    15181 tcgggattgg agtcttactg ctcggcgcaa tcctctatca tgcgcggcac ctgctggggc
    15241 ccgcggaact gagcggcgtg gaactgctgc gggcgatccg cggggcgagg ctgtctttgc
    15301 taatggcctc gttggcggcg atttacgtgt gctatgtgct gcgcgccctt cggtggatga
    15361 gcttttcacg ccatctggga ccggtctcct tttggaatat ctactcgatg acgctggcag
    15421 gattctctgt gctattcctg ctggggcgag cggccgatcc catccggccg ctgctgctgg
    15481 ccaggaaaga acggcagccg gtggcagaca cgatggggat ttacgcgctc gagcggttgt
    15541 ttgacacagc gagcacggca gtcatcgcag cgataggtct gttcctcttg ccgacgcacg
    15601 gaggcgggga tggggtcccg aacgagttgg agcgagcggc gcggacggca ggcacgattc
    15661 tgatcgcggg agtgctcgcg gttgcggcgg tgctggtcta tttgcgcctg catgggacgt
    15721 cgctgctgga gcggcggctg gagggctgga gcacgacgcg gggctggcgg tccgtggcgg
    15781 cgcggatcgt gctgggcttc gtgcgaggga tccagaccat ccggagctgg ggcgacttag
    15841 ggtccgcggt attctactca gcggcgcact gggggttgat cgtggtgatc tacttttggg
    15901 tcacgcggag ttttggagga aggctggggg agatctcttt cagcggggcg actctggtga
    15961 tggccttcac gatggtgggg tcaaccgtgc aattaccggc agtgggcggg ggctcgcagg
    16021 tgggctcgat tgttgcgtac acggcgattt ttggagtaca aagagagccg gccgtggcgg
    16081 cggcgattgt gttgtggctg attacgtttg cggcatgcag cctggcgggt gtgccgctgt
    16141 tgatccggga agggttgtcg ctgggagaac tgcgaaaaat ggcggtgcgg aaagagaagt
    16201 aggatgcccg ggcggagaag gcagcgagga gaaaccggcg agtgaagtgc ccgttttgcg
    16261 cgcgcgttga cgacaaggtg attgattccc gcgaaggccg cgctggcgac gcgatccgcc
    16321 ggcgccgcga atgcttgaaa tgcggccggc ggttcacgac ctacgagcgg atcgacgaaa
    16381 ttccttacat ggtgatcaag aaggacgggc gccgggagcg cttcgagcgg cccaagattc
    16441 tccaggggct gctgaaggcg tgtgaaaaac ggccggtggc gacgctgaag ctggaaggga
    16501 tcgtggagga gatcgaagga gtggtgcacg aggccaccga gcgcgagctg acgacaacgg
    16561 agatcgggga gatgatcatg caccggctga agaagctcga caaggttgcc tacgtgcgct
    16621 tcgcgtcggt gtatatggat ttcaaggacg tgaaggagtt catggccgaa ctgaagggct
    16681 tgctaaagga ccggtcgaaa aagtaatcgg cctgcttggg ttttcaggag acagatcatg
    16741 aacaagatga gagctcggct gagcttttat ggcttgctgt cggcgttctt gctgagcggc
    16801 gcggccgggt attttgcggc gtacgcggcg gaagagtccg cggggcttcc gcgcgcagca
    16861 agtacggcaa tgcgcgggat cgacgcggag aggatccgcg ccacggtgaa gtttctgtcg
    16921 gatgattccc tccagggacg cggcaccggc cagaagggcg gcgacgtggc cgctgactgg
    16981 atcgcggagc agtttagagc ctacggcttg cagcccgccg gcgataacgg ttccttcttc
    17041 caagatgtga agttctacgg agtcacaacc gatcagcaga agacgcagct cgctttcgtc
    17101 cccaagtccg gcgcgcagct cacgttgaag ttcgcggacg actatgttgc caccgaccaa
    17161 acccattccg ggcaatcaca aattgacgcg cccctggtct acgtcggcta tggcatccat
    17221 gcgcccgagt accagtggga cgattacaag ggcgcggacc tgaaaggcaa agtcctgctg
    17281 atgctggtga atgagcctcc ctcagacgat cccaacttct tcaagggcaa ggcgctgacc
    17341 tactacggcc gatggacata caaatttgaa gaagccgcgc gccggggcgc agtgggagtc
    17401 atcttgattc acaagacaga gatggccagc tatggctggg aagtggtgcg caactcctgg
    17461 ggcggtgaaa gctcgattct tcaggatgaa aaaggcgcga agctgaagtc ggcagggtgg
    17521 atccagctcg aagtggctcg aagactcgct caggctgcgg ggacggacct ggacaccatg
    17581 atgcagaacg ccacgtcgcg caacttcaag ccagtggaac tgcccatcca ggtgaaagaa
    17641 accatcgtga gccatgtgcg ttcgttctct tcgcgcaacg tagttggcaa agttgccggg
    17701 tctgatccca aactccgcaa ccaggccata ctttacacgg cacattacga tcatctgggt
    17761 atccatccca atgagccggg ggacaacatc tataacggcg cggccgacaa cgctaccgga
    17821 tgcggcatcc tgctggaact ggcgcgggcc tttgccggag ccaaagagag acctgggcgc
    17881 acaattcttc tggccgccgt caccgcggaa gagcagggcc tgttagggtc gaagtatctg
    17941 ggcgagcatc cgcccattcc ggcgcgcaac atctcgctgg acttgaacta tgacgatgtg
    18001 cagccgtttg gcgatcctca acaggtagtc gtttccggcg ccgaaagaac gaccatctat
    18061 cctttggtcc aagaggtcgc gaaagatttt gacctcagca tccagccgga cagccgtcct
    18121 gaggccggcc attattatcg ttccgatcac ttcagcctgg cgcgtgtcgg agtccccgcg
    18181 ttcagcgtga atgaaggtgc gctgttcaaa ggccatgatc ttgcctgggg agaggagaaa
    18241 gagcgcgatt atgtcgccca ccgctaccat cagccgagcg atgagtacaa gccggagatg
    18301 gactttacgg gcgacgcaaa gatggcgcga ttcggatttg cgctgggatg gaaagccgcg
    18361 acgatgccgg gcctggcggg ttggcagcct ggggatgagt ttgagcgagc gcgaagagag
    18421 agccagaagc cgtaagcaag atcctcttga cgagtccggg cgttgaggtg atgggtggga
    18481 agcgggacgg ggctgtgcta gaatttttca tgcgcgcccg tagctcagct ggatagagcg
    18541 tctggctacg aaccagaagg tcgggagttc gagtctctcc gggcgcgcca ttgcttagat
    18601 gttcttcgtt tacattctcc gcagcaaatc caaccagcgg cactacaccg gccatgcgac
    18661 cgatctgagt cagcgtctgg gccagcacaa cagtggcatc accaaatcga cgaagaatcg
    18721 cggcccatgg gagctcgtgc atcacgaaga gtttacgacg cgtgcggagg cgatgcggcg
    18781 ggaaaagttc ttgaagtccg gccagggccg ggaagaggtc cagcggatcc tccgaaacgc
    18841 tcgtagctca gctggataga gtccgcctgc ggcggaaacc agaaggtcgg gagttcgagt
    18901 ctctccgggc gcgccattcc aagacgtgac ccttctttca atcagcgaag cttctgatcg
    18961 aatgaccatt ctggatttgt tccatttgag tacactgaga agtccggctg atcgaaagtt
    19021 tccagaactg ttggattgag cagcatcgcc cctggcggca gaatctgcca attggggctg
    19081 tttgaactcg cctgcccata aaagatcatc tctcggctgc gtgtttcgga tgcagctctt
    19141 gctgcgaact tcgaatcctt ccccgcctgt cgccgcgccg cttgtatcag cttttctgcc
    19201 gtgtcggcgt cgaagccttc cacggcaatt accagatcgt tgtagagctc aatgaagata
    19261 ggcccatctg aactggagta cttttctctt ccgggtttcg ccgcttcggg ccgctcgtgt
    19321 tccagcttgc tgtaacgctg gggcagggcg gcagcgtaaa tcctggcaaa ctcctgggct
    19381 accttgtcgc tggaccattt ggagacataa agcaacccga tgtgggcggt ggagttcgcg
    19441 tcgctgggtt tggctccctt gcggccggcg gcatagtagg ccccgccacg ccattcgggg
    19501 ctgaggcggg cggccaccgc gttgtccgca tactgcttga gcagaatgct tacgtccaat
    19561 tcgcccacag cgccggcgtc gaatgcctca aagtccttct tgagaaagtc gaaatccggc
    19621 agtagcagcg gcgggacaac acgtccggca agatactcct ggggctcaag aatttcccgc
    19681 gaagtccggg gcatgcgctc caacactccg gaaaatgcca gcttccggcc tccggcgatc
    19741 agcagctccc ggatgaacct catgcccgcg ccataaggaa agacgagttc ctccttgagg
    19801 agaagaggag cactgtcaaa taatgtgcct cccgcgttct gttccagttc tcctcgcatc
    19861 atgtccagga gtttcgggga atcttccacg ctgtggccca tcggcttgag cagataatcc
    19921 acgaacacga tcatggcctg tccttccatg accgccgtgc gggcggtgga cttctcatca
    19981 atcctgatca gggcgttggg atcctgagga ccgcgttttt cgatctcttc ctcttccttc
    20041 atcatcttgc ccagatcaaa actttggtct tgaagggcat gcgtcagctc atgggccatg
    20101 accgccttct gcatgtccaa ttcaacccag tcgagcaaat tgatggtttt ggtcttctcg
    20161 tcgtagtaac cggccacctg ttctcccatc agcttgataa gaaagtcgtg aagttcaaac
    20221 ttgcgcggga gcagcccgaa cttcttcagg accagttcgg agcgctcaaa gcggatgcgg
    20281 tccacgtcgt ctttgaactt gtctccgata tatttctcca cctgggcgcg attcaccatg
    20341 gctttcttga ccggaaactt cacgggaagg agggtatctg cgctggcgaa acggagaatc
    20401 tcgtcaacgg attgaaaaag ctccctggct tcttccgaag tgaccttgtg ctctttttgc
    20461 ggctgctcct ggtcaagacg agccggagac ggagctgctt gctgacgcaa cggcggggtg
    20521 gtttgggccg caacagggaa agcggagagg aacaacaaaa gtgaaacaat ggatattttt
    20581 ttcatcggga agcggaaagt actgctagga ctccgctcct gacaatagcg cattgcgttc
    20641 gttcctgcga ggaatggaat cattggccgt caggggctac gttcttcttc gttttatttt
    20701 tccttgggag gtcaggacag aatgtccggt tatgatctag gctcggggaa tcagcctgct
    20761 ctgctgggtc ttctcgttcc attgccggcg caggttcgac ttgaggtagg attcgccggg
    20821 acgggtgaaa agctcggact cgcctctgcc gaaactccgt gctcgacagt tgcggccgga
    20881 aagtgcaaaa ttccgtctgc tctggggcaa agtaggcagg ggaagtcgag cgaattgcga
    20941 ggagggtcgc catgaatgaa tctgggaaag catcgaatcc cgtccggttt tctgcggtgg
    21001 ccagcttggt gttatcggcg ctgctcataa gcttggtggg cgcgcaggtt gcggcggcgc
    21061 aagggggcga tgcgattacg attggcgtgg tactgcccat caccgggcgc gaaggcaaac
    21121 tagggcaata ccagagggaa ggcatcgagc tggccatcaa gaagatcaac gacggcggcg
    21181 ggatttccgt gaaagggaag aagtacatct tcaaggaagt cttctatgac gacggctcag
    21241 attcggccaa gtccgccagc ctgacggagc gggccatgag ctcggacaat gtgacggccg
    21301 tgattggcgg ctattccact gcactggggg aggccgagtc cgtgatggcg gaccgctacc
    21361 agacgccgtg gatcacaccc ggcgccgcgg ctaccagcat ctttagtcac ggctaccagt
    21421 atgtctttgg atctttgagc cccatcgccg tgctgggttc caccacggcc gaattcctgg
    21481 gaagcctggt ggaccagggc aagctgaaga aggggctcaa gatcgccttg gccgtcgaga
    21541 atacggacca cggtatcgat tacgtcaacg ggatccagga ctggatcaaa atgcatccgg
    21601 gctatttcac cgtggtcttc agcgaaaagt tcgagctggg tggaactgac ttttccggtc
    21661 tgttacagaa ggtgaaatcc gtcaaggccg acatcttcct gagcgacgcc cacttgcagg
    21721 actacatcac catgcagcgg cagtatctgc aaagcgggat gcaccaccag atgataagtt
    21781 atggcgcgcg aggtccggag gccgatgccc gcaaggcgct gggcgatggg atcaattaca
    21841 tctttgccgc gatctggtgg tcgagcaaat tgtcttatcc gcaagtgaag cagttcatca
    21901 cggactacaa acaattcacc gggcatgatc ccgactcctg gtatgcggtc cccgcatatg
    21961 actcggtgcg catcctggcc gcagccatca gccaggctgg gtcaaccgac aagaccaaga
    22021 tacgggatgc tctgcgcaac gtatcgttga aagattcact cctccccggc accgtcctga
    22081 agttcgggcc gaacggacaa accggctatc catttgtcat cgtacaaaac cagccgggtg
    22141 gaaaggtgga gatcgtctat ccccgaaatg gcgccactgc agcacccatt gcgcccactc
    22201 cctagcgcgg ccccgggaca tccatgaccg cggttgacta tctcaacctg ttgatcgcgg
    22261 ccctgctgct cgccggcatc tacgccgcga tgtcggtggg gatgacggtc atctacgggg
    22321 tgatgaagat cgtgaacctg gcccacgccg ggttcctgat gctgggcgcc tacttcgttt
    22381 acgaactctt tgaacggttc cacattgatc cgatcctggg agcggtgctc gccctccccg
    22441 tattttttgc cttcggcatg ggcgtgcact ggctgctggt gcggtggctg cccaagtcgg
    22501 acacacccac tctcgcctcg ctgctgttga tgttcggact ctggctcgtg ctgcagaatg
    22561 tcggctatct ggtgtggggc aatatggacc gctccatctt cacgccgcgg acgctcagtg
    22621 tgcagcgctt cggcgagatc accatcccca cgcttggact gatcgtgttt gcggcggcag
    22681 tgatcagctt ggccgccttg cagatcgtct taaaccgaac ctggttcggt cggtcgatcc
    22741 gggcgctgat ccagaatccg tacgcggcgc agatcgtggg cgtggatgac cagaagaccg
    22801 ccaggctcac tttcggcctg gggaccgcct tcgccggttt tgccggcgcc ctcctggcca
    22861 tgctcttttc cttcaacccg gacttcggcc ggcagttcct gctccgcgcc ttcgttatca
    22921 ttgttctcgg cggcctggaa tcggtttccg gagtagccct cggcgcgctg attctcgcgc
    22981 ttgtggagac cttcagtatc ctgaaacttc ccgccggcta ccagcctgcg atttcttttg
    23041 cgctgttggt ggtggtgttg gtcatcatgc ccaaggggat cgccggcctg ctggaaaaga
    23101 aatggcgggt gacatgaaga tatccgctgc cctgtcccgc cggagcgcgg ttattttccc
    23161 ttgcctgctg ctgatcgttc tgctggcgct gccggcggca gggctcagcg ccaacatggt
    23221 ccggatgctg ttcctcacgg cagtctggac catcaccagc ctgagctgga acctgctggg
    23281 cggttttgcc ggccaagttt cgtttggatt cgccgtgttt tacggactgg gcgcctattc
    23341 cacggcgctt ctgatgcacg cgggtgtaaa tccctacgtt tccttcctcg gagcggcagc
    23401 cacggccgcg ctggcgtcgg ttgtcatcgg cctgccgacg tttcggctgc gcggcccgta
    23461 ctttgcaatc gccaccatcg gcgtgactga ggccgtgcgt gtggtgatga acaatctgga
    23521 catcaccgga ggggcgagtg gctatcgcgt tacggaacct gggccctttc gcccgctcga
    23581 acattactac acggcgattg cagccgtggc tttcgcagtt gtggtttccg tcgtcatttc
    23641 cagaagccgc ttcgggttgg cgctgcgggc catcaagcag gatgaagatg ccgccgcgga
    23701 cctgggcgtg aatccctaca cctgcaaact acggatccac gccgtggctg ccgcactgac
    23761 cgggattgcc ggcggcatct atgcccggcg tgcggccttc attcatccgg gagacgtatt
    23821 tgcgttccag accggcattt ccatcctgct catgccggtc atcggcggaa tcggaacagt
    23881 ctggggggcg gtgctgggcg gcgtgacctt tggcttggtg gagcaggagc tggtggcgaa
    23941 tttcccccag gtccatctgc tgctgtatgg ttccttgtta atattgatcg tcttgctgga
    24001 gccgaatggc ctggtgggac tgctgcgaag actcttcagg agaacacgtg aagattctgg
    24061 aagtcaggat gctgacaaag tattttgggg gcgtggcggc cttgaaggac gtaagctttg
    24121 aggttcgcga aaagcacatc tgcggcttca tcggccccaa cggtgccggc aagacaacgc
    24181 ttttcaacgt gatcaccggc gcggttcgcc cctcggcagg catggtccat ttgcgcggca
    24241 gcgacgttac cggcctgcgc tccgccacgc tggtcgcgag aggcgtggcg cgcacccatc
    24301 agatcgtccg gccgttccgg gcgctgaccg tgctggaaaa cgtccaggtt gccatccatt
    24361 acgggcgtcg gtcggtaagg cgtaccgctg cggctcgaga atacgccatg gatgtgctga
    24421 aactggtggg cctggagcac aaagctcaac ggcaggcctc ggtgctggcg cttggcgagc
    24481 acaagcgcct ggaagtggcg cgggcactgg ccaccgcccc ggatctcctg ttgctggacg
    24541 aaatctgcgg cggcctgacc actgccgaga cggcctccat gctggatttg ttgcagcgca
    24601 tccgcgaaga cggcgccacc atcatgtacg ttgagcacga tatgaaagct gtcatgtccg
    24661 tctgcgacca catcaccgtg ttgaacttcg gccgaaagtt ggctgaagga aagccgcacg
    24721 aaatccagaa cgacccggcc gtcattgagg cctacctcgg taaagccacc ttcgggagcg
    24781 cgccgcgtgc ctagtggcca ggacgcaaat ctactggtcg ccgacagcct ggaggtgcgc
    24841 tatggcgtgg tgcaagtcct ctggggaatc tccttctccg tggcccgcag caaggtcact
    24901 tgcatcatcg gttcgaacgg cgcggggaag accaccacgc tgaataccat tgccggagtg
    24961 cttccggtca agagcggccg cattttgctg gacggggaag acgtgacttc tttgcccccg
    25021 catcaacgcg tgaaacgcca catcaacctg gtgcccgaag gacgccagct ctggccggga
    25081 atgtccgtgg aagagaacct gctcatggga tgtttcctcc ccgggctgcg aggtcacgct
    25141 aaagctggcc tagcgcgggt ctacgatctg tttccgcctt tgaaagaacg gcgccggcag
    25201 ctggccggca cgctttccgg cggcgaacag cagatgtgcg ccatcggacg cggcgtgatg
    25261 actgagccgc agctcttgat gctggatgag cctaccctcg gtttggcgcc caaattggtg
    25321 gacgagatct tcgagctgat aggcaatatt gcacgccagg gggtgaccat ccttctggtg
    25381 ggccaaaacg tgaattacac gctgcaagtt tcccactatg gctacgtgat ggaatcgggg
    25441 cggatcgctc tggaaggacc gagtgaaaag ctccgcaacg acgatcatgt gcgccgggcg
    25501 tacatggggg cccatgatta gcgcggcgat gttcgcctta ctgcgcggcc actggtgatt
    25561 gtttttgccg ttttgcgcga aaccgggcaa tcagagtatc aagggagaac aatcctggtc
    25621 caaaaaacaa gaccacgagt gatgcgcaca agaacgtgaa gggagctgcc gcgtagaact
    25681 tgcccggatc cgagaagatc gaactcaggg cttcccgatc ggcaacgacg tacgccacga
    25741 tcatgtcaat cgccagcggc acggcaatca accgcgatcc taatccgagc acaagcagaa
    25801 tgccaccgcc cacttccagc agtgcgatga aataagcatt gaaggcaggg gcagggaccc
    25861 ccagatcggt gaagtagccg atgactttag ggatatcgga gagctttccc aggccagtct
    25921 ggaaaaactg ccatccccag tacagccgaa tgacgagcag gagcggtgac tgaagcaaat
    25981 tcgccgcctt tagaaaccat gcataggcag aatcggcggc ttttatcaac gaattcacgg
    26041 ccccatcagt tttcgggctg cttgcagaac catcccaggg acgaccagtt ggcaaaccag
    26101 ccttgcagtc gttcggtgat gcgctcaaca ggttggttgg tccaatggac ggaagtctcg
    26161 atcgcctgcg agagaatctt gccctgctgc aacgcgcgga tcagggcaaa ggcctcccgt
    26221 tccagccgtt tgaaatagac gacatcgtcc tgccggtgga ctgccagaaa tacttttttc
    26281 ggccggggca aggataatcg tctaccgcga gcccgcgggg tccgctccag cacaacattg
    26341 ctggcgatat cgctttcttg ctcttcattc tgacggacac tcaggagcaa gtcgtccact
    26401 gggtaggcca ggtccagcag gcgcaggtag ggctgcacgt ggaaacttgg atcctctccg
    26461 agattgcgaa gatcgccttc cgcaagttta ggtaactcag ctccgtcaaa tgcttcgata
    26521 tcggcccatt caagccgcac catgtcgagg gccatgcgcg atgtcttggg aacgaacttc
    26581 gggtgatcgc gcagccattc ctccagccgg gccccgaggt tccgcagaga gaaggattcc
    26641 gacgggcaat cgctcaggta agcaaccagc agtttatcga aattccgctg cccgataatg
    26701 gcccgcagcc ccggaaaatc ttcggcgagt gccgaaagaa tgcggaacca gtacacgcgg
    26761 ttgtagattt ccagccgttc gaatgaggtc agccggtcat tcggcttgat catttcctcg
    26821 acgatcttct tgacaggctt tccgtcgcgt gtccgcttgc gcatgccttc cgaggcagtc
    26881 agcggctgtc ggatcacgtc gaacaccctt cgctgaagtt gctccaggtc catcacgccc
    26941 ctattttctc cgccacgtaa gggttggacg ccgcacgctg cttgggcccc aggcgctgat
    27001 tggcgagcac cggctgtttg gccttgatga atttcttagc cttcaaggct tcctggtgga
    27061 cttcctcgaa agacggaatg cgatcgtccc attccagaag cgttgccgtt ggcccggcca
    27121 gttcaatcgc tcgggcgtaa agctgccaaa ccgggtcgag caccgggtga tcgtgagtgt
    27181 ccagaatgta tttttcgaac ttcgaatgtc ccgcgacatg gatttgcgcc acgcgcgcgg
    27241 gcggaacgct gttcagataa tcgaaaggat tgaagctgtg gttttgcgaa gacacgtaga
    27301 tattattcac gtccagcagg atgccgcagt cggcctcttc aaccacttca atcaggaact
    27361 cccactccgt catttcggaa acgtgatact cggcatagct gctgacgttc tcgacggcga
    27421 tcggcacttc aacgaagtcg cgcacctcgc ggattttctg cgctgtcttc tttgccgctt
    27481 caaaggtgta cggcatggga agaagatcgt gcgagtagcg gccgtccacg ctgccccagc
    27541 acaagtggtc ggtcagccag ggagtgttcg tgcgcttcac cagggtcttc agccgccgca
    27601 agtgttcgcg gttcagcggc tccgcggagc cgaaatacat ggagaccccg tgctggacaa
    27661 cgcgatattg atcgaggatc tggtcgagca cacgcagcgg acgcccgcca tccaccatga
    27721 agttttcaga gataatttcg aaccaatcga cgaccggttt cttttcgaaa atgtgctgat
    27781 agtgagggat gcgcagccct atgcccacgc cgtattccgt atgtccattg aaccgatttg
    27841 ccggcataaa gataaattgc ggatgaatgc agtcgtatca aaggtagagg gccagaggtt
    27901 tgcttccact ggccctctac aggagaaacc ccagctaact cttcggcatg gggtgtttcg
    27961 ggtcggtgga gcacccaccc ttgcccttgc aatcgttctt gcctttggtc gtcccgcagc
    28021 cccccttgcc cttgcaagag ttcttccccg cacaggaatg tatttcaacc ttcttcttct
    28081 ttgttgcctt cttgccggag tcgctcttgg gggccttttc gtccccggtt cttgccatag
    28141 cagcagtgct gccggcaaca aaccctgtca gcgctgctcc gaccagcagt gctcgcatcg
    28201 atttcgtcat ctgtaaatct cctttcctct gcttattctg cgcctgacga gagcttcccg
    28261 gattgcttta tcgtgcaccg aacaagctca ccagggactc tcggcagact ctttcttctc
    28321 agcccgaaca cccggattga tcacaacgag tcatccactt cttgaatcca gcagggttca
    28381 ctcgacctct gatacctggt gaattccaga gcgccaacgg gtacatagct tggcgcggga
    28441 ggatggtaac acgaattcta cgaacattgc gcaagaactg ttgaatcagg gttaggggag
    28501 gatttgcgac cctctttttg tcgtgtcgtc ttcgatccca aggggactgg ggagccattg
    28561 gctttgaaca ttgacagcgc cggggcggtc cgcacatgat agccacgcct cgcgcgaatc
    28621 cccgacccgc gcactgcgcc gggcggcgag ttcggcgggc acaaataaaa tgatggttga
    28681 catttttcga aaacattagt aatagcgaac aacgtatgga ttcatctcgt tcccccaact
    28741 tccgcccact cactctgatg ggccgtgagc ccggtggggg aaggcccggt agataccaac
    28801 cgatccggcg agcagagata gccgcagaga gattcaaacg acggatttcg gaaccaggga
    28861 atttcggaag gatttgaaga ggaggacgga catggcaaaa aggaaaaaca acgggtttac
    28921 acgccgtgat ttcatcaaat ggagcgggag cggggccatc gcgctcggcg ccggcagcgg
    28981 accgttcttt ttgtttccgg ggaaggccgc ggcacagcag aaaactctga agatctgcca
    29041 gtggagccac tttgttcccg cgtgggacaa gtggttcaac gagacctaca ccaaacaatg
    29101 gggagagaaa cacaacacca acgtgatcgt tgacaatatc aacctgaccg aactcaagac
    29161 gagggccgcc tctgaaattt cagcgaagaa aggccacgac cttttcatgc tgctctcgcc
    29221 gccggcggca tacgagaaac aagtcatcga catgacccac gtttaccagg aggtggagaa
    29281 gaagcacggc aagaaaatcg acctggcgca taaatccacg tacaacccca agaccaagaa
    29341 gtactttgcc ttttctgacg cctacactcc cgatccggga aactggcaca aggactggtg
    29401 gaccgaggct gggtatccca acggcccgga cacatacgac gatctgctgg cgggcgccag
    29461 gaagatccgg aagtctgaca agagcggccc tccctgcggc ataggactcg ggcaggaact
    29521 cgataccagc atggcgatgc gcgccatcct ctggtccttc ggcggagcgg agcaggacgt
    29581 cgaaggcaac gtgaccatca attccaaaaa caccattgaa gcgctgaaat tcatgaaagc
    29641 gttgtaccag gacacagaaa cgcaggaggt cttcacctgg acgccgccct ccaataacca
    29701 ggctatgctc gcgggcagag tttcctttgt gatgaacgcc atctccatca cccgccagtc
    29761 cgagagagaa catctgccca tcgacagcaa gatcatggtc agccaggcgt tgaaaggccc
    29821 ggtgcgcagg atcgccgccg agcacgtcat gaattgctac gtgatctggg agttcgcgga
    29881 aaacaaggaa ggcgcgcagc ggttcttgat cgactacatg gacagcttcc acgacggttt
    29941 tgtcgccggg cagttttaca acttcccctg cttccccaag accgttccca acctgaagca
    30001 ggagattgcc aacgattcgc gcgccaaccc gccggacaag tacaaggtgc tgggcaacgt
    30061 gctggagtgg gccaccaacg tggggtatcc cggttacgcg accgcgggca ttgacgaggc
    30121 cttcaccacc tgggtgattc ccaccatgtt cgctaacgtc gcccgtggtc aaagcactcc
    30181 tgaggaggcg gccaagatcg ccgaggccga gtacaagcgt atctttgcca gaaatacggg
    30241 ggcttgatgg cggtcgttga gacgcgaagc ctgaccaaga tatttaaaga aggagagctc
    30301 ggagccgtca acgacgtaaa tctggtaacc cgcgagggcg agtttctggt ttttctggga
    30361 ccttcgggtt ccgggaaaac cactctcctg cgcatgatcg ccggcctgga aactcccacg
    30421 gccggcgaaa tcttgattgg cggccaggta gtcaaccacc tgacgcctag agagcgccgg
    30481 attgccatgg tgttccagag ttacgctctc tatccgcatc tcaccgttta caagaacatt
    30541 gcctttcccc tgaaagcgca aaacgtacct aaaaaactgc accgagaaaa agtcgagtgg
    30601 gcagtgtcat tgctgggcat cagcagcctg ttgcagcgca agccgcgcga actctccggc
    30661 ggcgaacggc agcgggtggc cctggcccgc gccatcgtgc gcgagccctc ggtctttctg
    30721 ctggacgagc ctctatccaa cctggatgcc aaactccgtc tctccgcccg cgaagagctg
    30781 gagcgctttc accgccggat tgggaccacc accatctacg ttacgcacga ccaggtggaa
    30841 gccatggcca tgggcgaccg tattattgtg ctgaacaagg gcgaggtacg gcagttcgga
    30901 acgcccaagg acgtctatga cgagcctgcc gacacttttg ttgccacgtt cctggggtcg
    30961 ccgcccatga acctcgtgga gaacggtgag gtcatcgtgg gctttcgccc ggagcacttc
    31021 cggccggcgg agaccgtcac agaagccaga ttcacgttca agtatcgcgt ggaaaacgtg
    31081 gagtacctgg gcgctgagta cattctctca ggctttttgg cgggtggacg tgccgaaggc
    31141 aagaaggcca tcgcccgcct cctgctggga caatccttcg agatcgggac cacctacgag
    31201 ttcgccgtgc cggagcggca cttgaatttc ttcgatcgca ccagcgaaaa acgggttgcg
    31261 gcgagggccc tggtgtggca ataggccggg ccgggattag atcgatcggc gccgctcaga
    31321 gccccctctt gggcgccgcc atgttcggcc ccgcggtgct ctacattgtg ctcctcatcg
    31381 gtttgccgtt tatattgtca attctctacg cgttcagcga tgcccgcatc ggaagcatcg
    31441 ggtttcatct cgtcggcctc gaaaacttcc gcagcattct gcagagtccc agttttcgca
    31501 cggcgctcaa gaactccgtc attttcactg tctgcgcgca agttatcgta gttgtctgtt
    31561 ctacgacgct gtccatcgcc ttggaggaga agttccgcgg ccgcggttta gtgcgctttc
    31621 tgatcctgct gccctgggtg gcgccggtct ccctcggcac cattgggtgg aagtggatcc
    31681 tggattccat ctacagcgtg atcacctggt tgctgatcca ttttcatttt gtggggacgt
    31741 acagcgctcc catgtggctg ggcgagccgc atctggccat gacttccgtg attctcgtgc
    31801 attgctggcg gctcatcccc ttttccacgg tcatcatgct ggcaggtcgc tccgccattc
    31861 ccaaagagat accggaggcg gccgccattg acggcgcagg tttctggcgc acgcttttcc
    31921 agatcagtat ccccatgatg gcacccatca tcagcgtggc cgtgttattc ggcaccatct
    31981 tcaccttcac ggacatgacc gtggtttaca tactgactgc cggcggacct tttgactcca
    32041 cgcagacctt gccctcgctg gcctttgcga cgggtattca gggcagcgac cttgccgccg
    32101 gggccgccat ctcgattttc ctggtgcccg tgctggttgt gattgcctac ttgatgcttc
    32161 gtgtggcgca ccgcgcggag gtggtctgat gaaaaaggca acttcgcgca agcttggccc
    32221 cagagtcgca cgttgggcag ttctgtcagt cttcgtcgtg ctgctggctt ttccgttcta
    32281 ctggatgttc attgcggcct tcaagcagaa catcgacctc tacaacgtag agagcaaccc
    32341 ttttatcttt aatctcaaac ctacgctgga tcatctgcgc ctgctgttta atgaaaccag
    32401 gtatgtgcga tggctcggca atacggccct ggtgggcgtg ctcgtggttg ccatcacgct
    32461 gctgctgtct gtgccggccg cttacgcgct ggcccgtctt accggccgct ggggccagcg
    32521 cctgggaatc ggcatctttc ttacttatct ggttccgccc acgttgctct tcatccctct
    32581 ttcccgtgtt gtggccatcc tgggcctgca ggacacgatt tggtcgctgg tcctggtcta
    32641 ccccagcatc acggttccgt tttccatctg gctcctgatg ggttttttca agtccattcc
    32701 caaggaactg gaagatgccg ccatggtgga cgggcttacg cgcttcggcg ccttcatcaa
    32761 gatggtggtt ccggtctcca tctccggcat tctgacggtg gtgatcttca ccttcacgct
    32821 ggtgacccag gaatttgttt acgcactgac cttcatctct ccggaatcgc aacagatggt
    32881 cggggtcggc attcccatct tcctggtgcg cggggatgtt tacttctggg gttcgctgat
    32941 ggcggcctgc ctgatcgcca gcttgcccat cgcattcttc tataacctct tcctggaccg
    33001 cttcatttcg ggctttacgg ttggcgctgt gaagggctga gccccaagca cgtgtaggcg
    33061 caaggctagc cttgacaatg caggagattc ggggtaaaga aggcgtgaaa ttgcacctct
    33121 aggcacggct tccacgcaga ggaggctcca gccaatggcc acgagtcgca gggacttttt
    33181 caagatcagc gcagtcggtg gtgttgctgc ggcagtgctg ggtttcgacc tggcgcccgc
    33241 gtacgcgcaa ttgcgcgcgc tcaagattgc ccgcgcgacg gagacgcgtt cgacgtgtcc
    33301 gtactgtgcg gtgagctgcg gtgtaatcat ctacaccatc ggagacaagg cgaaaaatgt
    33361 tacgcctcag gtgctgcacg tggagggtga cccggatcac ccgatcaacc gcggcactct
    33421 ttgccccaag ggcgcttcgc tcatgcagga catcatgaac acgcggcggc tgatcaagcc
    33481 ggaagtccgc cgtcccggct cggaccactg ggaggagatt ccctggaacg acgcaatcaa
    33541 cgagatcggc gggtggatca agaaaacccg cgacgcgacg tttatcgaga aggatgcgaa
    33601 gggaaacacc gtcaaccgtt gcgagagcat cgcgttcacg ggcgggtgca cggacacgaa
    33661 tgaattgaat tacctcgtcg tcaagacgat gaggagcttg ggactcgtcc acttagaaaa
    33721 ccaggccaga gtttgacacg gccccacggt ctcaagtttg ggaccaacat tcggacgcgg
    33781 ggcaatgacg aacggatggg tggacttccg gaattccgac atgatgctga tcatgggcgg
    33841 gaatccagcg gagaaccatc cctgcgggtt caagtgggcg ctcgaagcga agcgcatccg
    33901 gaacgcaaag atcatttccg ttgacccgcg attcacgcgc acttccgcgg tcgcggacct
    33961 ctattgtcat ctccgggcgg gaacggacat cgcgttccta ggcggaatga ttcgctacgc
    34021 gattgagaac aaacgcatcg cgcacgatta cctggtcaac tacaccaacg cggccttcat
    34081 cgtaaaggac ggcttcaagc tgcccgagga cgggctcttc tccggcttcg atcccgccac
    34141 gcaggtctac gacaagtcca cctggaacta tgaagagggc ggggacatgg ccggaaaaga
    34201 ggaccatccg gctccggccg cgaagcccgt tgccgcccct gcgggcggaa aacccgcgcc
    34261 tcctcccgcg ctgcctccaa acgtggccta cgacctgacc ctgcagcatc cgcgctgcgt
    34321 gttccagctc ctgcgcaaac aatactcgcg gtataccccg gagatggtcg agcgcatcac
    34381 gggcgctccc agggagcagt tcctcaaagt cgccgacatg ttcacgtcca ttcgcaagga
    34441 cggcgacacg aagaaggtgg ccacggtcat ctacgcgctg ggctggacgc agcactctta
//
LOCUS       DASYVO010000301         1917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_62043, whole genome shotgun sequence.
ACCESSION   DASYVO010000301 DASYVO010000000
VERSION     DASYVO010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62043"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1862)
                     /locus_tag="VGR03_10230"
     CDS             complement(213..1862)
                     /locus_tag="VGR03_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV1994697.1"
                     /translation="MAKILVADDNSNIQKMVGLALKDQGIDVVAVGNGEAAVRKISDL
                     RPDLVLADVFMPVRNGYEVCQYVKSDPSLAHIPVILLVGAFDPLDEQEAQRVGADGVL
                     KKPFVPPDPLISMVKSALQRAGVAHGNASAAKTPEPEPLRASDLLTPSGASRPPIASI
                     PVQATSPEPAIAALDQESFVDEVPARPAPVRIDSGSQTVAFGSLLETPADEKDEDIGF
                     PPPVHPELATERDWSDPDAGETDEEAEEEKPAAPWRREDAESYADAGSTSGAKDWRSG
                     GFEQILAGKTKVESWEPADEKPELVGTAPAASALVAEASPATIAEATKLTGTISSTPF
                     ANDAWAAASSPASQEKSSTETATAVEEVAGVKHQEQKSAEALVASAPQVPPSEPQTKT
                     SKESWFSVPSNPWNVEMEKANRLASAWDAAVPVAAPPVSSNGTEKATVAVSETTQKVI
                     EQAALPAAAVEAIREEAAQVAEDAAHAAEQHNSDGTSFYAPEALVEVPAPTPAPTPSM
                     DDLVARVLARMSPEVLQAVTREILKPVVEAMVKEEMKLKKS"
BASE COUNT          345 a          622 c          510 g          440 t
ORIGIN      
        1 ctttgctatc tcgcgagcca tccggtgcgc ttctccagga aagagttccg gcgcgactgc
       61 cctacaagcg ccaaatcttg ttcagagtaa tcttcgacta tacgggggga aaagcggctg
      121 cgtcaattcg gccgggcgct gccctgcgaa gcaacattca atattgatca gtacaaagcg
      181 tgaatggatt tgccaaacgt tcgggcaggg aattaagact tcttcaactt catctcttcc
      241 ttgaccatcg cttccacgac gggcttcaga atctctcgcg taaccgcctg cagaacttca
      301 ggactcatcc tcgcaagcac tctggcgacc agatcgtcca tgctcggggt cggcgcaggc
      361 gtcggcgctg gtacctctac aagtgcctca ggcgcgtaaa agctagttcc gtcggagttg
      421 tgttgctccg cggcgtgcgc tgcgtcttcg gctacctgag cggcttcctc gcggatggcc
      481 tccacagcgg ctgcaggcaa ggccgcttgt tcgatcacct tctgagtcgt ttcgctgacg
      541 gccactgtag ctttttccgt tccgttgctt gacaccggag gagcggccac cggcacggca
      601 gcatcccacg ccgaagccag ccggttcgcc ttctccatct caacgttcca cggatttgat
      661 ggaacagaaa accacgactc cttggatgtt ttcgtttgcg gctcggatgg cggcacttgg
      721 ggcgcgctag ccaccaacgc ttccgccgat ttttgttcct ggtgcttcac acccgctact
      781 tcttccaccg ccgtggctgt ctccgtcgaa ctcttctcct gcgatgcagg cgaacttgcc
      841 gctgcccacg cgtcgttcgc aaatggcgta gacgatatcg ttcctgtgag ttttgttgct
      901 tccgcgatcg tggccgggga cgcttctgct acaagagcgc ttgctgcagg cgccgtccca
      961 acgagttccg gcttttcgtc tgccggctcc caggactcca cctttgtctt ccccgctaaa
     1021 atctgctcaa aaccaccgga gcgccaatcc tttgcaccgc tggtggatcc ggcatcggca
     1081 tagctctccg catcctcacg ccgccacggt gcagcgggtt tttcttcttc cgcttcctca
     1141 tccgtttcgc ccgcgtccgg atcgctccaa tcgcgctccg tcgccaactc aggatgcacc
     1201 ggcggcggaa aaccgatgtc ctcatccttc tcgtccgcgg gtgtttccag gaggctcccg
     1261 aaagctaccg tttgcgaacc actatcaatc cgcacgggcg cgggacgcgc cggtacttca
     1321 tctacaaaac tttcctgatc taaagctgca atggcaggtt cgggtgaggt cgcctgcaca
     1381 gggatactgg cgatcggcgg cctgctcgca ccacttggcg tgaggaggtc tgaggccctc
     1441 aaaggttctg gttccggagt tttcgcggcg ctggcgttcc cgtgggccac tcccgcgcgt
     1501 tgcagcgcgg acttaaccat cgaaatcagg ggatctggcg gaacaaacgg cttcttcaac
     1561 acgccatctg ctccgactcg ctgggcttct tgttcatcca gcggatcgaa cgcgcccacc
     1621 agcaggatca ccgggatatg cgctaacgag ggatccgact ttacgtattg gcaaacttca
     1681 tatccattcc gcaccggcat gaatacatcg gcgagcacca ggtcggggcg caaatcggag
     1741 attttccgca cggctgcttc gccgttgcca acggcaacga cgtcgatgcc ctgatctttc
     1801 agggccagcc ccaccatctt ctggatgtta ctgttgtcgt ccgcaaccag aatcttggcc
     1861 accgaacgtc ctccgacgat actctacctg tatagaccac acttagcgcg cagccat
//
LOCUS       DASYVO010000302        12752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_205728, whole genome shotgun sequence.
ACCESSION   DASYVO010000302 DASYVO010000000
VERSION     DASYVO010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 12752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12752
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205728"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..975)
                     /locus_tag="VGR03_10235"
     CDS             complement(226..975)
                     /locus_tag="VGR03_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994698.1"
                     /translation="MENSKGLGRIFVAISLVVFGVQHFIYGGFVAGLVPAWMPGRLFW
                     AYFVGVAFFAAAAGILYQGMARPAATMLGVMFFLFVALLHIPRIAVHSSDGNEWTSGF
                     VALAMCGGAWILASASPLEERETADPFLKFGRYIFAIAFVAFGIQHFVYARFAAGLGP
                     PWFSGRPLWAYLTGIMFVAAGARIVLGKNTRLAAALLGTILFLFFLLLYVPRITAQLH
                     NPGPWTSGFEVLALCGGALVLAGTRPREKRE"
     gene            complement(1099..1644)
                     /locus_tag="VGR03_10240"
     CDS             complement(1099..1644)
                     /locus_tag="VGR03_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1994699.1"
                     /translation="MRRERDNTSYRIEPLRENHNRAAFSCGVEARDRYLQKQAGQDIS
                     KHVAAAFVITPNGETIAGFYTLSAHVVSLGDLPESVAKKLSRYPNVPATLLGRLAVSK
                     DFRGRGIGELLLLDAFKRVLVHSSEVASAAVVVDAKDERARHFYLAHDFIPLPSQPNR
                     LFYLVKTIEKLFEIKSERERP"
     gene            complement(1641..1946)
                     /locus_tag="VGR03_10245"
     CDS             complement(1641..1946)
                     /locus_tag="VGR03_10245"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEV1994700.1"
                     /translation="MTLALKKQARQVRKIERVEARLNPDQKRRIEYAASLAGTSVSDF
                     MVSSADDAAARAIEQHEVWTLTGRDREVFVKALMRPPAPSTRMRAATRHYRSRVRAS"
     gene            2105..2446
                     /locus_tag="VGR03_10250"
     CDS             2105..2446
                     /locus_tag="VGR03_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994701.1"
                     /translation="MRGINIDSLSEEELVELNHKIVARLRFLGQMRSHSVMLDFRIGE
                     KVKFRPTGRPELIGTLTRYNKKTVTVITESGEHWNVAPGLLSRLDSGASGNVDTPSST
                     VVQFSVRAPKK"
     gene            2658..2801
                     /locus_tag="VGR03_10255"
     CDS             2658..2801
                     /locus_tag="VGR03_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994702.1"
                     /translation="MQRKVFWTTFIALGLVADFTMPIVWSLLATIPIGIFSWWVAYRS
                     DWF"
     gene            complement(3331..4533)
                     /locus_tag="VGR03_10260"
     CDS             complement(3331..4533)
                     /locus_tag="VGR03_10260"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV1994703.1"
                     /translation="MKEFERIFWIVLDSVGIGELPDAADYGDVGRNTLGHIAESRPLE
                     IPNLVRLGLANIAPLKHQSPATSPLGAFGKGATISPGKDTTTGHWEMAGVRLEQAFPV
                     YKNGFPKELIEEFEKLIGKKTIGNKPASGTEIIKELGEEHVRTGKPIVYTSGDSVFQI
                     ATHEDVIPIAELYRMCEIARKLLDGPHRVGRVIARPFTGTAGNFVRTTRRHDYAVDPP
                     KPMLMDVLAERNVRVFGVGKIHDIYNGRGVEEYVTTKNNTDGMEKMTAAVRERKSGLI
                     FCNLVDFDMLYGHRKDVEGFAKSLEEFDRLLADFLPLLSLSDMLMITADHGCDPDPRW
                     ATTDHSREYVPILAYSRGGGASVNLGVRDTLADMGQTIAENFGGTILHGKSFLSELRK
                     QRSNEVAR"
     gene            4770..5714
                     /locus_tag="VGR03_10265"
     CDS             4770..5714
                     /locus_tag="VGR03_10265"
                     /inference="COORDINATES: protein motif:HMM:NF028425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HEV1994704.1"
                     /translation="MAQDGNPRRVSLVFPIILIAVGALFLFHNWRPAFDPWPILATYW
                     PLILIFIGIGKIWDTTYRSRNPNAPQGISIGATIGTLAFVIVLVVLLWHGRSYSRSHG
                     YYSGSQHNTQTVDLQGAKSAHAKLELGAGQLTINGGANHMLDADFTYDGSFDAPRVDY
                     SVSGGVGQLNISQESHSAHFGRSQNDWNLHFSKDIPLELKVDMGAGQGNLHFRDVPLT
                     RLDLNIGAGQMDVDFTGDRKTDLTADIEGGVGQANIRLPKNVGVIAHASGGIGSIDVH
                     GLKHDGDSYTNDAYGKSPATIHLKVEGGIGQITLTQEP"
     gene            5838..8819
                     /gene="uvrA"
                     /locus_tag="VGR03_10270"
     CDS             5838..8819
                     /gene="uvrA"
                     /locus_tag="VGR03_10270"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV1994705.1"
                     /translation="MATKEITHATTDVRPEQEFIRVRGARVHNLKNISVDIPHNAITV
                     VTGVSGSGKSSLAFDTIYAEGQRRYVESLSAYARQFLERMEKPDVDEISGIAPAVAIR
                     QKNSTRNPRSTVATATEIYDYLRLLFARCGQTFCIKCGAEVRRDNPDQIATRILSLAA
                     GRRFYVLHQFRVAASASTAVTKRGAKKTVAAPAPELLRQALLDLQKRGFNRLYQAGRI
                     HEFSSPETLLDADFSKPVYVLVDRLAVNPESRTRLVDSIEICYREGQGEAILEFVADS
                     AGNSAERLTFNERFECKNDGTLYQESEPRLFSFNNPYGACPRCQGFGNTIDFDLNLVV
                     PDPSKSLDDGAIEPWTKPRYRVLFQDAKKWARGRGIPTNVPWRQLTAEQRRLILEGDP
                     ENDYQGIKGFFTWLERKKYKLHVRVFLSRFRGYATCPECGGTRLRAEARAVRVAGRAI
                     TEVCKLTVKEARAFFSTLQLSEGHLKIADRILEEIRTRLQFLDEVGLDYLTLDRLTST
                     LSGGESQRIQLATSLGSHLVGALYVLDEPSIGLHPRDTQRLIDILKSLRDLGNTVLVV
                     EHDPDTIRAADCIIDLGPGAGELGGKLLFAGSYAAMLEEHKSLTGRYLSGELRIPVPN
                     VRHKPAGRFLRLYGASLHNLQDMDLMIPLGTLTVVTGVSGSGKSTLVHDVLYKALTTK
                     RVGGSVKDFCERLEGDANVQEVVIVDQSPIGRTPRSNPATYLKAFDAIREIFADTPDS
                     KRRGYAAGHFSFNVPGGRCETCQGDGTVTVEMQFLADVELICEECRGTRFKSAVLEVR
                     YHGKNVHEVLQLTVREALAFFVNVPKIVSKLRVLNEIGLGYLRLGQSATTLSGGEAQR
                     LKLAAHLSRTDNEGILYILDEPTTGLHFDDIAKLLSAFRKLLEGGGSLLVIEHNLDVI
                     KSADWLIDLGPEGGDQGGKIIATGTPEQVARNTQSHTGHFLARVLNGNGQAKRPTNGA
                     SKSAGPSATISNGTKIPPAEP"
     gene            8816..10177
                     /locus_tag="VGR03_10275"
     CDS             8816..10177
                     /locus_tag="VGR03_10275"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1994706.1"
                     /translation="MKLTHLRSIAIGLLLCGFCVGGAQHAAPLQSNQSPANAQSSRPK
                     PAAQAKSQKVANPLNDLLEEAQRDIDKNNFEVAIAPLQKVIAEQPEFAYAHFQLAYVY
                     TALKRTDEARAEYARTIAIDPKMSEAYLNLGMMLLDKQEEVAAVAPLRKAVELLPAQS
                     RPRYLLAVALDRSGDRAGAAESFEALLHLDPNDITAIDYLAWGALRKGKPEEAEARFR
                     RALEVQPNGPEARRGLAQSLDAQKKAEAAGAYREYLELMPNDSEARARLIHLLVEEKE
                     NDEALAELDRLDVGKQPTLEALKLRADVQIAGKKWDGSIATLQQALALAPNDAQLHGG
                     LGRILLQKRDFAAAEKELRVALRLDGKNLTYFKDLSSTFFLGGNYPAALATLDEIAKV
                     EQPGAGVWFVRAICYDKLNQPKLALEAYQKFLALDRDKNPDQVWQAKERSKVLQRMLE
                     RKR"
     gene            10182..10694
                     /locus_tag="VGR03_10280"
     CDS             10182..10694
                     /locus_tag="VGR03_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994707.1"
                     /translation="MRASGTSLGAVFGLLVFLAILPPGAAESLADLQARFDRETSGVQ
                     KAKLIGKLGDAQFEEARRAGKAGDYNTVGLTLEKYRDNVGAAIEALKKQHPDAEKQSN
                     GYRQLEMHVRKSIREVDETLLVSPEAYKPPLQLVRQDLIGMDEELLKMLFPRRPLEQH
                     AATPPAEKQR"
     gene            10691..11212
                     /locus_tag="VGR03_10285"
     CDS             10691..11212
                     /locus_tag="VGR03_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994708.1"
                     /translation="MKYKLHLFLLASCILAASPLVRAATPQQKDYLSSLEADKIRDAE
                     TVNERIKLFLTFADDRLKKFQYELDHPSTNRHGDMLNSLLNAYVGCVDDAADLIQLGI
                     EKQDNIRQGIDLMTARTKEFLVVLEKLSTDGAERELYKDNLDDAIEGTRDAMNDAEKA
                     KKKVAPPPVRRKN"
     gene            11216..11956
                     /locus_tag="VGR03_10290"
     CDS             11216..11956
                     /locus_tag="VGR03_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994709.1"
                     /translation="MRNLTTAMQSSGRRTSMVPGGGALFQRMFTRLGCDGRPPRFRVE
                     FYPYSSLVLTIRRREEVVHVRFSDLLRRAPLAVLESAAVLLLARVYRRKASQALTEPY
                     LEYARSNRTRSRMNLMRRGRVRLAATSPRGEHFDLDGLFDQLNGKYFADQLQRPHIGW
                     SARSWRRQFGCYDPGPNQILLNRRMDRPGVPACAVEYVLFHEMLHVKHPTRRSGCSMV
                     SHSREFREEEKRFAEFERARGILDRLAR"
     gene            complement(11983..12474)
                     /locus_tag="VGR03_10295"
     CDS             complement(11983..12474)
                     /locus_tag="VGR03_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994710.1"
                     /translation="MTGMRFQKTIPILAAVFLLCATLLARAQDPPQESSSKPAEAPAA
                     APPKSSAKQKKDTATQNAPDQPAWDPLRAEKDLEVGQYYMKKGDLDAAIDRFQDATVA
                     KPGYAIPFRCLAEAQEKRGLKKQAIKSYQRYLDLYPHAEDGDKVRKKIDKLYKEVEKE
                     KKQ"
     gene            12553..>12752
                     /gene="uvrC"
                     /locus_tag="VGR03_10300"
     CDS             12553..>12752
                     /gene="uvrC"
                     /locus_tag="VGR03_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrC both incises the 5'
                     and 3' sides of the lesion. The N-terminal half is
                     responsible for the 3' incision and the C-terminal half is
                     responsible for the 5' incision; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit C"
                     /protein_id="HEV1994711.1"
                     /translation="MEPREKAAQLPESPGVYLFKDAGGTVLYVGKAGNLRSRVRSYFL
                     ESRWIDAKTGSLAREIADLETIV"
BASE COUNT         2665 a         3799 c         3532 g         2756 t
ORIGIN      
        1 tgctcggagg tacggcctgt ttcgcgcctc ccggccctcc ggactgggca cgtggcaggc
       61 cctcaacggg tgggcacccg ccatgtattt ctttccgaaa tctgctccct acccggcgcg
      121 agatgtctcc ttcttgcaca agcccactct tgctagacgg gcagcctgcc ccgatcatca
      181 agtcggggcg gcagcccacg ttctttgcgt acatcgtttc gctacctact ctcttttttc
      241 tcttggccgg gtgcctgcca ggaccaaagc gcctccacac agggccagga cctcgaatcc
      301 gctggtccac gggcccggat tgtgcagttg cgccgtgatg cgcggaacgt aaagcagaag
      361 gaagaacaga aaaaggatcg tccccaggag agcagccgcc aatcgagtat tttttccgag
      421 aacgatcctt gcgcccgcgg caacgaacat gatgccggtc agatacgccc aaagtggccg
      481 tcctgaaaac cagggcgggc ccagccccgc tgcgaaacgc gcgtaaacaa aatgttgaat
      541 cccgaaagcc acaaaagcga ttgcaaaaat atatcgcccg aatttgagga acggatcagc
      601 ggtttcgcgc tcttccaagg gagaagcgct ggcaagaatc caggcgcctc cacacattgc
      661 cagcgccaca aacccgctgg tccactcgtt tccatcgctg gaatgaacag cgatcctggg
      721 aatgtgcagg agcgcgacga aaagaaaaaa catcactccc agcatggttg ccgccggtcg
      781 cgccattccc tggtagagaa tgccggcagc cgctgcaaag aacgccacgc ccacaaagta
      841 cgcccagaac agccgccccg gcatccacgc gggcaccagt cccgccacga aaccgccata
      901 aatgaaatgt tgaaccccga aaactaccag ggaaatggca acgaatattc gcccgagacc
      961 tttgctattt tccatgatgg ccaggcctgc tctcgctttc agtttgtgtc cacaacattc
     1021 agcagtctac atgagttaag gcctgccagc gtggcttgcg tataacaccc gagtccgagt
     1081 cagccatcct ccagcggctt agggtctttc acgctcactt ttgatttcaa atagtttctc
     1141 tatcgttttc accagataga aaagccggtt cggctgtgac ggcagcggaa taaaatcgtg
     1201 cgccagataa aagtggcgcg ccctctcatc ttttgcgtca acgaccacgg cggcggatgc
     1261 aacctcactg ctatggacca acacacgctt gaacgcatcg agcagcaaca gttcgccgat
     1321 gccccgccct cggaagtcct tgctgaccgc cagcctgccc agcagcgtcg cagggacgtt
     1381 tgggtagcgg gagagtttct ttgccacgct ctccggaaga tcgccgaggc ttacgacgtg
     1441 ggccgacaaa gtgtagaaac cggcgatggt ctctccattc ggtgtgatga caaaagcagc
     1501 cgctacatgc ttgctgatgt cctggccagc ctgtttttgc aaatagcggt cccgcgcttc
     1561 cactccgcac gaaaaagccg ctcgattgtg gttctctctg agcggctcga ttcggtagga
     1621 agtattgtcc cgctcccgcc tcacgaagcc ctcacgcggc ttctgtaatg ccgggtggcc
     1681 gccctcatgc gtgtactcgg ggccggcgga cgcatgagcg ccttcacgaa cacctcacgg
     1741 tctcggccgg tcagcgtcca tacttcatgt tgttcgatgg cccgcgcagc ggcatcgtcc
     1801 gcgctggaga ccatgaagtc cgagaccgag gtgcctgcaa ggctcgcagc atattctatg
     1861 cggcgtttct gatccggatt cagccgcgct tcgactcgct cgattttcct gacctgcctg
     1921 gcctgtttct ttagcgccaa agtcatggca tccctcgcca ttccaattgt acggtaattt
     1981 accgtacaat tccatgaaag gaacgcgcaa cgccgttcca taagtacgtt tcgcgagtgg
     2041 tttcaacttg ccacacggcc cggtttctat acaatacgtc cagtggttcc aggatcggac
     2101 ccccatgcgc ggcatcaata tcgattcgct ttccgaagaa gaactcgtcg aacttaatca
     2161 caaaatcgtg gcgcgcctca ggtttctggg ccaaatgcgc tcccacagcg tgatgctgga
     2221 tttccgcatc ggggaaaaag tgaagttccg ccccacgggg cggcctgagc tgattggaac
     2281 tctcacccgc tacaataaga agaccgtcac tgtcattacc gagagcggag agcactggaa
     2341 tgttgccccg gggctgctct cccgtctgga ttccggcgcg agcggaaacg tggacactcc
     2401 gagttccacc gtcgtccagt tttcggttcg cgcgcccaag aaatgaattc gttggagggt
     2461 ttttggggtg tggctcagaa tcgatccgct agccagggtt tgggtgccca gccctcgatt
     2521 ggcgtttacc ccgactcctg tcgggggggt ggggcctttg atttttcctc tatcctcttg
     2581 ccccgcgatg tgtttcttct cgaaatctgg tccatggccg gccaccgcac ggcgattgtc
     2641 ggcgctagaa taaaagaatg caacgcaagg tcttctggac taccttcatt gcgctgggac
     2701 tggtagcgga tttcaccatg cccatcgtat ggtcgcttct tgccacgatt cccatcggca
     2761 tctttagctg gtgggtggcc taccgcagcg actggttctg acgcaggaag tcttagttgg
     2821 tcctattcct cgaccccttt cttcctgcac caatatgtaa tcgttgttga caatctattc
     2881 acgcctctgg tacactatcc ccgttcagta gttcgcctcg cctcgccttc cgcgagctcc
     2941 ctcttacaac tcaattcttt cttttctgga gacttccatg tccaccaatc gctccgtcat
     3001 gccttcagcc gctcttcttc ctcagcgctc ctggcaatca tcgaacggcc gaaatcgcac
     3061 tttttgtact aacccattta gaatcaccac ttgcaaaaat gtgccaaaac acgcctccaa
     3121 ctatctttag aatcaacact tataaaaatg ttcattctaa aggactttaa ccttctttag
     3181 aatcaacact tatgaaaaac atggggggtg ggggggtatg attaaccaga cacccgatga
     3241 agggtgtctg tcctgaggag catcgcgacg aaggacctcg ctcccgacag tcacaggtca
     3301 cgagtcacca gtcacgctta tcgcttttcg ctacctcgct acctcattac ttcgttgctt
     3361 cctcaactca ctcaaaaagc tcttgccgtg cagaatcgtt ccgccgaaat tctccgcgat
     3421 cgtctggccc atatccgcca gcgtatctcg cactcctaag ttcacgcttg cgccgcctcc
     3481 ccgcgaatac gccaaaatcg gcacgtactc ccgcgaatga tccgtcgtcg cccagcgcgg
     3541 atcgggatcg catccgtggt ccgcggtgat catcaacatg tcggagagac tcagcagcgg
     3601 caagaaatcc gcaagcaggc ggtcaaattc ttccagcgac ttggcaaacc cctcgacgtc
     3661 tttccggtgg ccgtaaagca tgtcgaaatc caccagattg caaaaaatca gtccgctctt
     3721 gcgctcacga accgccgccg tcattttctc cattccgtcg gtgttgttct tggtcgtgac
     3781 gtactcttcg actccgcgac cgttgtagat gtcatgaatc ttgccaactc cgaaaacgcg
     3841 cacgttacgt tccgcgagaa catccatcag catcggcttc ggaggatcca ccgcataatc
     3901 gtgccgccgc gtggtccgca caaagtttcc tgccgttccc gtgaacggcc gcgcgatcac
     3961 tcgccccacg cgatgtggcc catccagcaa tttccgtgcg atttcgcaca tgcggtacag
     4021 ctcggcgata gggatcacgt cttcatgcgt ggcgatctga aacacgctgt cgcccgacgt
     4081 gtacacaatc ggcttgcccg ttcgtacgtg ctcctcaccc aattccttga tgatctccgt
     4141 accggaggcc ggcttgtttc caatggtttt cttcccgatc agtttctcga actcttcgat
     4201 caattccttc gggaacccgt tcttgtaaac cggaaacgct tgctccagcc gcacccccgc
     4261 catttcccag tgtcctgtcg tcgtatcttt tcctggagag atcgtcgcgc cctttccaaa
     4321 cgctccaagc ggagaagttg ccggcgactg atgtttcagc ggtgcgatat tcgccagtcc
     4381 gagccgcacc agattcggga tttccaacgg ccgcgattcc gcgatgtgtc caagcgtgtt
     4441 cctgcccacg tcgccataat ctgccgcatc aggaagctcg ccaattccca cgctatccag
     4501 cacgatccag aaaattctct cgaattcctt catattccgg cccctaaagt cccattcgcc
     4561 cgcactctac cacaccgcct gctcagccaa tgctcagcga tagtttcggt cttatgtgga
     4621 ggccgggctt cagctcggct cttcgtcccg ccgtcttcca gccggctctt tcagttcttg
     4681 ccagcacctt cgttgttgta cagtctaacc gtccgctcgc tgaactattt tccttccgcg
     4741 tgcgtagtac aaggtagcgg aggtgtgcca tggcccagga cggtaatccc aggagagttt
     4801 ccctcgtctt cccgatcatc ctcattgcgg tcggtgctct ctttctcttc cacaactggc
     4861 gtccggcttt cgatccctgg ccgattctcg cgacctactg gccactcatc ctcatcttca
     4921 tcggaatagg taagatttgg gataccacat atcggagtcg caatcctaac gcgcctcagg
     4981 gaatctctat cggcgccact atcggaacgc ttgcctttgt cattgttctg gtcgtcctcc
     5041 tctggcacgg acgcagctat tcccgcagcc acggctacta ttcgggatcg cagcacaaca
     5101 cacaaactgt ggatctgcag ggagctaagt cggcgcacgc caagttggag ttgggcgccg
     5161 gacaactcac catcaatggt ggcgcgaatc acatgcttga cgcggacttc acctacgacg
     5221 gctctttcga cgccccgcgc gtcgactaca gcgtgtccgg cggggtcggc cagctcaaca
     5281 tttctcagga gtcccattcc gcgcacttcg gccgctcgca aaatgattgg aatctgcact
     5341 tcagcaagga catcccgttg gagttgaagg tggatatggg ggccggacag gggaatctgc
     5401 attttcgcga cgttcctctc acccggctgg acctgaatat cggggcgggc caaatggatg
     5461 tcgacttcac cggcgaccgc aaaaccgatt tgaccgctga cattgaagga ggcgtggggc
     5521 aggccaacat tcgccttccg aaaaacgtcg gcgtcattgc gcatgcttcc ggcggcattg
     5581 gttccatcga cgtacatggt ctgaagcacg acggggactc ctacaccaac gacgcttatg
     5641 gaaaatctcc agcgacgatt cacctgaaag tggaaggcgg aatcggccag atcacgctca
     5701 cgcaggagcc gtgaatttgc cgggcaaatt cgccaccgcc tacgtccatg gagaatcgcc
     5761 gtttgctccg ctttgacagc agacgtataa tttgaagttt aggcagtgcg ctcgaaacgc
     5821 gctgtctggg cttccccttg gcgacgaaag agattaccca cgcaaccacg gatgtccggc
     5881 cggagcagga gttcatccgc gtccgcgggg cccgcgtcca caatctcaaa aacatcagcg
     5941 tggatattcc gcacaacgcc attactgtcg tcaccggcgt cagcggctcc ggaaaatcct
     6001 ccctcgcgtt cgacaccatt tacgcggaag gccagcgccg ctacgtcgaa tcgctctccg
     6061 cgtatgcccg ccaatttctc gagcgcatgg aaaaaccgga tgtcgacgaa atttccggca
     6121 ttgccccggc ggtggccatt cggcagaaaa attccacgcg caatccgcgt tcgacggtag
     6181 cgaccgccac ggaaatttac gattatttgc ggctgctttt tgcgcgctgc gggcaaactt
     6241 tttgcatcaa gtgcggggcg gaagtgcggc gggacaatcc ggaccaaatc gcgacgcgca
     6301 ttttgtcgct tgcggcgggg cggcggtttt acgttttgca tcaatttcga gtcgcggcat
     6361 cagcaagtac ggcggttacg aaacgaggcg ccaagaaaac cgttgccgct ccggcgccgg
     6421 aacttcttcg tcaggcgctg ctcgatcttc agaagcgcgg attcaaccgg ctttaccagg
     6481 cgggtcggat ccacgaattt tcgtcaccgg aaacgctgct ggatgcagat ttttcgaaac
     6541 cggtgtatgt gctggttgac cgtcttgcag tcaatcctga atcgcgcacg cgccttgttg
     6601 actccatcga aatttgttac cgcgaagggc agggcgaagc cattctggaa ttcgtcgcag
     6661 acagcgcggg gaattccgcc gagcgtctta cttttaacga acgatttgaa tgcaagaacg
     6721 acggcactct ctatcaggaa tccgagcctc ggcttttctc gttcaataat ccatacggag
     6781 cgtgtccgcg ctgccaggga ttcggaaata cgatcgattt cgatttgaat ctggtcgtcc
     6841 ccgatccgag caagtcgctc gacgacggcg ccatcgaacc ctggacgaag ccgcgctacc
     6901 gcgtactttt ccaggacgca aaaaaatggg cgcgggggcg gggaattccg acgaacgtgc
     6961 cgtggcgtca gctcaccgcc gaacaacgcc gcctgattct ggagggcgat cccgaaaacg
     7021 attaccaggg aatcaaaggt tttttcacct ggctggaacg gaagaaatat aagctgcatg
     7081 ttcgcgtttt cctgagccgc tttcgcggtt atgcaacttg tccggagtgt ggcgggactc
     7141 gtttgcgcgc cgaggcccga gccgtgcgcg tggcgggaag agccatcacg gaggtttgca
     7201 aactcactgt caaagaagcg cgtgctttct tttccactct gcaactgtcc gaagggcatc
     7261 tgaaaatcgc ggacagaatt ctcgaagaga tccgcacgcg tctgcaattt cttgacgaag
     7321 tgggtctcga ctacctcacg ctcgaccgct tgacctccac gctctccggc ggagaatcgc
     7381 agcggattca gctcgcaacg tcactcggtt cgcatctcgt tggcgcactc tacgtgcttg
     7441 atgagccttc catcggcctg catccacgcg acacgcaacg gctcatcgat attctcaagt
     7501 cgctccgcga tctagggaac acggtgctcg tcgtggagca cgatcccgac acaattcggg
     7561 ctgcggattg catcatcgat cttggtcccg gcgcgggcga actcggcggc aagctgctct
     7621 ttgcgggctc ttatgcggcc atgctcgaag agcataaatc gctaacgggc cggtacctca
     7681 gcggggaact gcggattcct gtgcccaacg tgcgccacaa accggcgggc agattcctcc
     7741 ggctttacgg cgcaagtttg cacaacctcc aggatatgga tttgatgatc ccactcggga
     7801 cgctcacggt ggtcaccggc gtcagcggct ccggcaagtc cacgctggtt catgatgtgc
     7861 tgtacaaagc gctcaccacg aagcgcgttg gcggcagcgt caaggacttc tgcgagcggc
     7921 tggaaggcga tgcgaacgtc caggaagtgg taattgtcga tcaatcgccg atcggccgca
     7981 caccgcgttc gaatcctgcc acgtatctga aagcgttcga cgcgattcga gaaatttttg
     8041 cggatacgcc cgattcgaaa cgccgaggct atgctgccgg tcatttttcc ttcaacgtgc
     8101 cgggaggccg ctgcgaaacg tgccagggcg acggcacggt caccgtagag atgcagtttt
     8161 tggcggatgt cgaactcatc tgcgaggagt gcaggggcac gcgcttcaaa agcgccgttc
     8221 tggaagtccg gtatcacggg aagaatgttc acgaagtatt gcagttgacg gttcgcgaag
     8281 cgctggcgtt tttcgtcaat gtccccaaga ttgtttccaa actcagagtc ctgaatgaaa
     8341 ttggcttggg ctacttgcgt cttgggcagt ccgcgacaac gctttccggg ggagaagcac
     8401 agcgtctgaa gcttgccgcg catctcagcc gcaccgacaa tgagggcatc ctctacattc
     8461 tcgacgaacc gacgacgggt ttgcattttg atgacatcgc aaagctgctc agcgcgttcc
     8521 gcaagctgct ggagggcggc ggttcgctgc tggtcatcga acacaatctc gacgtcatca
     8581 aatccgcgga ctggctgatc gatctgggtc ccgagggggg cgaccagggc ggcaagatca
     8641 tcgccacggg gacacccgag caggtggcgc gcaatacgca atcgcacacg gggcattttc
     8701 ttgcgcgcgt cctgaacgga aacggccagg caaagcgtcc cacgaacggc gcatccaaat
     8761 cagcgggccc ttcggctacc atttccaacg gaaccaagat tcctccggcc gaaccatgaa
     8821 gctcactcat ctccgttcga ttgcgatagg gcttcttctt tgcggctttt gcgtcggagg
     8881 ggcgcagcat gctgcgcccc tacaaagcaa tcaatcgccg gcgaacgcgc agtcctcgag
     8941 gcccaaacct gccgcgcaag ccaagtctca gaaggtcgcg aacccgctca acgatttact
     9001 ggaggaagct cagcgcgaca tcgacaaaaa caattttgaa gtggcgatcg ctccgctaca
     9061 aaaggttatc gccgagcaac ctgaatttgc ttacgcgcac tttcagctcg cctacgtcta
     9121 caccgcactc aagagaaccg acgaagctcg ggccgaatac gcacgcacca tcgctatcga
     9181 ccccaaaatg tccgaggctt atctcaatct cggaatgatg cttctcgata agcaggaaga
     9241 agttgccgcg gtagcgccac ttcgcaaagc ggtggaactg ctcccggcac aaagccgtcc
     9301 ccgatatctg ctggcagtcg cgctggaccg ctcgggagat cgggccggtg ccgcggaatc
     9361 ttttgaggcg ctgctccatc tagatcccaa cgacatcacg gccatcgatt acctggcgtg
     9421 gggcgccctt cgtaagggca agcctgaaga agcggaagca agattccggc gcgcgctgga
     9481 agttcagccg aatgggcctg aagcgcgcag aggactggcg cagagcctgg acgctcagaa
     9541 aaaagccgaa gcggcaggcg cgtaccgcga gtatctcgaa ctcatgccca atgattccga
     9601 agctcgcgcc cggctcatcc atcttctcgt cgaagagaaa gaaaacgacg aggctctcgc
     9661 ggaactcgac cgtctggacg tcggcaagca gcccacgctc gaggcgctca agcttcgcgc
     9721 ggacgtacag attgccggga agaaatggga tggttcgatc gccacccttc agcaagctct
     9781 cgctctggcg ccgaacgacg cgcaactaca tggcggtctg gggcgcatct tgctccagaa
     9841 acgggacttc gcggccgcgg agaaggaact gcgtgttgcg ctgcgccttg atggaaaaaa
     9901 ccttacatat ttcaaagacc tcagctcgac gttttttctg ggaggaaatt atccggcggc
     9961 gctggcgacg ctcgacgaga tagccaaagt ggaacaaccg ggcgcgggcg tctggtttgt
    10021 ccgcgcgatt tgctacgata agctgaacca gccgaagctt gctttggagg cttaccagaa
    10081 atttctcgcg ctcgatcggg acaagaatcc cgatcaagtc tggcaggcca aggagcggag
    10141 caaggtgctc cagcgcatgc tggaacggaa aaggtaggcg gatgagggcc agcgggacgt
    10201 ccctgggtgc agtgttcggc ttgctcgttt tccttgcgat cctgcctccc ggcgccgctg
    10261 agagcctggc cgatcttcag gcgcgcttcg atcgcgaaac gagcggggtt caaaaagcta
    10321 aattaatcgg aaaactagga gatgcgcagt ttgaagaagc gcggcgcgcc ggaaaagccg
    10381 gcgactataa taccgtgggc ttgacgctcg aaaaataccg ggacaatgtt ggagccgcga
    10441 tcgaggcttt gaagaagcag cacccggacg ccgaaaagca atccaacggt taccggcaac
    10501 tggagatgca cgtgcggaag agcattcggg aggttgacga aactttgctg gtatccccgg
    10561 aagcctataa gccgccgttg cagcttgtcc gccaggatct gattggcatg gatgaagaac
    10621 tgctgaaaat gctttttccc cgccggcctt tggaacagca cgcggctacg cctcccgcgg
    10681 agaaacaacg atgaaatata aattgcatct tttccttttg gcttcctgca tcctggccgc
    10741 gtcgccgctg gtgcgcgccg cgacaccgca gcagaaggac tatctctcgt cgctggaagc
    10801 ggacaagatt cgcgatgctg agacggtcaa cgagcgcata aaactttttc tgacgtttgc
    10861 cgacgatcgc ttgaagaagt ttcagtacga gttggatcat ccgtccacga atcgccacgg
    10921 cgacatgctg aattcgcttc tgaatgctta cgttggctgc gtggacgacg cggcggacct
    10981 catccagctg gggatcgaaa aacaggacaa catccggcag ggaatcgacc tgatgacggc
    11041 gcgcacgaag gaatttctcg tcgtgctgga aaagctgtcc accgacggcg ccgaacggga
    11101 actctataag gacaatcttg acgacgcgat cgagggcact cgcgatgcta tgaatgatgc
    11161 ggaaaaggcg aaaaagaaag ttgcgccgcc acccgttcgg cgaaagaatt aaatcttgag
    11221 gaatctgacc acagcgatgc agtcgagcgg ccgcagaact tcgatggttc cgggaggcgg
    11281 ggcgcttttt cagcggatgt tcacacggct gggttgcgat ggccggccgc cgcgcttccg
    11341 ggtggagttc tatccctact cgagtctggt gctgacgatt cggcggcgag aggaagtcgt
    11401 gcacgttcgc ttctccgacc tcttgcgacg agcgccgctg gccgtcctgg aaagtgcggc
    11461 cgtgctgctg cttgcgcgcg tttatcgccg gaaagcctcc caggctctca ccgaacctta
    11521 tctggagtac gcgcgctcca atcgcacgcg cagccgcatg aacctgatgc gccgcgggcg
    11581 ggttcgcctg gcggcgacca gtccgcgcgg agaacatttc gatctggatg gacttttcga
    11641 ccagctcaat ggaaaatatt tcgcggacca gttacagcga ccgcacattg gatggagcgc
    11701 gcggagctgg cgacggcaat tcggctgtta cgatcccggg ccgaatcaaa ttctgctgaa
    11761 tcgccggatg gatcgacctg gggttccggc gtgcgcggtg gaatatgtct tgtttcacga
    11821 gatgctgcac gtgaagcacc cgacgcgccg ctcgggctgc agcatggtct cgcattcgcg
    11881 ggaatttcga gaagaagaga agcgctttgc ggaattcgaa cgcgcgcggg gcatcctgga
    11941 ccggcttgcg cgctgatgtg agtggccggg gaatgcccgg cttcactgtt ttttttcttt
    12001 ttcgacttcc ttgtagagct tgtcgatttt cttgcgcact ttgtcgccat cctcggcgtg
    12061 cggatataga tccagatagc gttgataaga cttgatggcc tgttttttca ggcccctttt
    12121 ttcctgggct tcggccaagc atcgaaacgg gatggcgtag cctggctttg ccaccgtggc
    12181 gtcctgaaaa cggtcgatgg cggcatccag atcgcccttc ttcatgtagt actggcccac
    12241 ttcgagatct ttttccgcgc gcagcggatc ccatgcgggc tggtcgggcg cgttttgcgt
    12301 ggcggtgtct ttcttttgct tggcagagga ctttggcggc gcagctgcgg gagcttcggc
    12361 tggttttgag gaggactcct gtggcggatc ctgcgcgcgc gctagcagtg tggcacatag
    12421 cagaaaaacc gcggcgagaa ttggaattgt cttctggaat cgcatgccgg tcacggaggt
    12481 ctccctgtac tccgattccg tctagtatga tggaagttgt ctggcgattg taaggttgtg
    12541 ttttgagcct ccatggaacc ccgcgaaaaa gccgcgcagc ttccggaaag cccgggcgtc
    12601 tatcttttca aggatgccgg cgggacggtg ctgtacgtcg gcaaggcggg caacctgcgc
    12661 agccgcgtgc gctcctactt cctggaatcg cgctggatcg acgccaaaac gggatcgctg
    12721 gcccgcgaga tcgcggatct ggaaaccatt gt
//
LOCUS       DASYVO010000303         7600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_62335, whole genome shotgun sequence.
ACCESSION   DASYVO010000303 DASYVO010000000
VERSION     DASYVO010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62335"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            594..2909
                     /locus_tag="VGR03_10305"
     CDS             594..2909
                     /locus_tag="VGR03_10305"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEV1994712.1"
                     /translation="MTVRLFCMIVCLLLIAPLQAQVDTPPYRDPQLALEDRVSDLLAR
                     MTLEEKVDQITGGRHESAGILDTTGQFPPEEMSEALKGLWQLDSHMSPHDRAVLHNAL
                     QRYLREKTRLGIPALFQGEALHGFMSYHSTSFPQALGLAATWDPALVKEVFTAAADEM
                     ASAGHNQAFTPVLDLARDPRWGRTEETYGEDPYLASRIGVSAIEGLQGPSFLIDRHHV
                     LATAKHFAAHGQPEGGTNTAPTNYSERILRESFLIPFEAAVKEARVGSVMASYNEIDG
                     IPSHVNHWLLDRVLRQEWGFRGYVTSDGEGLQMLVRVHQVAADNAAAAREALAAGVDY
                     DLSDGSVYRTLLDQVKQGLVPMAQLDRAVARVLAAKFRLGLFENPYVDPGYAEKITNS
                     PEHQKLALRAAQEEIVLLKNEHNLLPLDPRKLKAVAVIGPNAADVHLGGYARDPGRGV
                     SILEGIRQRLGSSAKVTYAEGCKITKGLQGWAGWYEDNTQQADPKAQVDSIRAAAAAA
                     KQADVAIVVVGETESTNREAWSEQHLGDRDSLDLLGAQDQLIQAVAETGVPTVVFLIN
                     GRPLSINYAAEHVPAILEGWYLGQEGGTAAAQVLFGDVNPGGKLPITFPRSVGQLPAF
                     YNHKPSRNRSYIFTSREPLFPFGFGLSYTSFRFDNLRVEPAEIAPGQSATVSVEVTNT
                     GDREGDEVPQLYIHQRVGTVTRPVKELRGFQRLRLRPGEKATVEFRLAPESLSMLDEH
                     MDRVVQSGMFELFVGSSSAQTSSVSLRVAQR"
     gene            complement(2959..4047)
                     /locus_tag="VGR03_10310"
     CDS             complement(2959..4047)
                     /locus_tag="VGR03_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994713.1"
                     /translation="MTDWKQITARIRRARTSKDPAGQLSNLYQKTRDAMVAFELAKFF
                     ETSGQNADAAKWYSAAAERFRRSDWKIKAQEAAVRLGGAPGEIPLEAGAPPTEEIALT
                     PPAVSTPEADLPFEQNPEALESSVAVTSEIQQAADNSMNAPASSEPERKGRRRGRRGG
                     RNRRKGSNGKEAAVTPGQHAAEAPAPRRGLPPAVFEESAEPTPPRVTHSRAIPTLPIE
                     PIGEPGGTGVKGRYGDPGLSSRISLLEMQFRRLMTCPSAKLEEADHAPAGPGVFVLTD
                     SDLTSYYYVEACQTLRIAIGNLLRGGSSRRGVDSIKPQLAQFLGIPEPRVAKYLGEHC
                     VVRWLQLDDGASNFAHFVIAVLRPALNE"
     gene            4201..4983
                     /locus_tag="VGR03_10315"
     CDS             4201..4983
                     /locus_tag="VGR03_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994714.1"
                     /translation="MIRLRVDCLTVTSSKDRLAVTSSMNLFAVTSRNDCISITKSILA
                     LFVSFSLLTTPVWAAPSSSLGIVVYADRAHVGAAQASVGATVFSGDRISTDQSGSVQV
                     RAGAARLLLSSASSATFSQDDASPTATLTFGSATFSTASSNAFALHVASAVIRPNSNQ
                     PTIGQVTVLNPKELIVKSTRGSLSIAVEDDVREIPEGAAYRIVLDPNAADPQGPRGAG
                     TKGHGGPPIKAAKSRFIWYAIAVTAVITYFALDEVFESPDRP"
     gene            complement(5502..6476)
                     /gene="nirK"
                     /locus_tag="VGR03_10320"
     CDS             complement(5502..6476)
                     /gene="nirK"
                     /locus_tag="VGR03_10320"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HEV1994715.1"
                     /translation="MPPEAVQAQTPEQAPIQTAVDIVRDPADVPPPVGKRQPQIVRVS
                     LIAQELEGKLDAVLDATYEYWTFNGKVPGPMLRVRQDDTVELTLKNDGRLVSHSIDLH
                     AALGPGGGMDLTEAKQGESRTFTFKAIVPGVFVYHCGTNLAAQHIANGMYGLIVVEPP
                     EGLPRVDHEFYVMQGEIYTVEDIGGTGHQKMSLSKLMDERPEYFVFNGAVGALTSQHP
                     MHAKVGETVRIFFGVGGPNLISSPHVIGQVLTKVYNEGSFTSPPLTSVQTTVVPPGGA
                     TIIEFKPLVPGSYKLVDHALVRVARSLIGAIEVSGPPHPELFHEGPAE"
     gene            complement(6799..>7600)
                     /locus_tag="VGR03_10325"
     CDS             complement(6799..>7600)
                     /locus_tag="VGR03_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994716.1"
                     /translation="VAGSFYVSFRGASPAFPHRFDQRYLALMGWGFLVPFVWGFSAKW
                     MRVFLGLKPLRPRLLLGAVLVNSTGVALAVAGKFWIATWVFVAGAGLSIAAIRVFERS
                     EQPAKIKGVHRTFPLFIRMAYGWLLVAALLGVAAAIWDTSGGIWGASRHALTVGFIAV
                     MVLSIGQRILPAFAGMRLLWSPKLMFAGLLVLSIGCTLRVSCEVLAYQGYAAWAWSVL
                     PVSALFELAGLTLFATNILGTFLLQPSHACNQPLVVGIPQLLTQELPD"
BASE COUNT         1586 a         2351 c         2117 g         1546 t
ORIGIN      
        1 cgcttgttga agtactgaat cgcgttgaac gtaattccag cggtcttgca aaccgcccag
       61 tcgatcgttc taaaaatctg cgtgcctact cgattcggca taaacctcta tcctccactt
      121 tcaagaatgc aaagtacttg gcccttgagt taaatggagc ccaatcactc gtcggagcca
      181 agcccaaacg actgcccccg ggctcacctt tgtgtgccta aaaaccgtac cacgaacacc
      241 aggtacgtcc aataagtctt tggactccta tagtttgacg gttacccggg gcggacgcca
      301 gcgggttgcg ccgaacggtc agattcgagg gtcgaacggc aatcccagcc agcgaacggt
      361 cattctccga gccccaaatg agggtaacta gtagtgcgaa agcattgcaa atttgggctt
      421 gcaagaaggt catccgcagt cccgatacga ggctcttccc aggaacggct ttcgctaatc
      481 ggtgagggaa ggacggagca cggcgctgac gaacaacgca gattgtgaag ggtcctcacc
      541 aagttaaaac cactgtgata taaggaagcg ccatctttcc atcgggggtg catatgacag
      601 ttcgcctatt ctgcatgatt gtttgtctcc ttctgatcgc ccctttgcag gcacaagtag
      661 acactccgcc gtaccgagat ccgcagctcg cccttgaaga tcgcgtctcc gatcttttgg
      721 cccgaatgac gctggaagaa aaagtggatc aaattaccgg cgggcgtcat gagagtgcgg
      781 gaattctcga cactacggga cagtttcctc cggaagaaat gagcgaggcc ctcaaagggt
      841 tgtggcagct cgattcgcat atgagtccgc acgaccgcgc cgttcttcac aacgctctcc
      901 agcgctattt gcgggagaag acccgcctgg gtatccccgc gctgttccag ggagaagcgt
      961 tgcacggatt catgagctac cacagcacca gcttcccgca ggccctcggg ctcgcggcaa
     1021 cgtgggaccc ggccttggtc aaagaagtct ttaccgccgc ggccgacgaa atggcatcgg
     1081 ccggtcacaa ccaggctttc acgccggtgc tggacctggc gcgcgacccg cgttggggac
     1141 gtacggaaga aacctacggc gaagatccgt acctggcatc gagaatcggc gtgtccgcga
     1201 tcgagggact gcaaggccct tcatttctca ttgaccgcca tcatgtcctc gccaccgcaa
     1261 aacactttgc cgcccatggc cagccggaag gcggcacaaa cacggccccc acaaattatt
     1321 cagagcgcat tttgcgggag tcgttcctca ttcccttcga agccgccgtc aaagaggccc
     1381 gcgtgggcag tgtcatggct tcgtataacg aaatcgacgg cattccctcc cacgtgaatc
     1441 actggttgct tgaccgcgtc ctgcggcagg agtggggatt tcgcggctac gtaacttcgg
     1501 acggcgaagg cctccagatg ctggtaagag tccatcaggt cgcggccgac aacgccgccg
     1561 cagcgcgtga ggctctggcc gcgggtgtcg actacgacct ttcagatggc tcggtctacc
     1621 gaacgcttct cgatcaggtc aaacaaggcc tggttcccat ggcccaactc gatcgcgccg
     1681 ttgctcgcgt gctagcggcc aagttccggc tcggactctt cgagaatccg tacgtcgatc
     1741 caggctacgc cgaaaaaatc accaacagcc cggagcatca gaagctggcg cttcgcgccg
     1801 cccaggaaga gattgtcctg ctaaagaacg aacataatct cctgccgctc gatccgagaa
     1861 aactgaaggc cgtcgccgtc attggcccga acgcggccga cgtgcatctc ggcggctacg
     1921 ctcgcgatcc cggccgcggt gtgagcattc tggagggaat ccgccagcgt ctgggatcca
     1981 gcgccaaggt gacttacgcc gagggatgca aaatcacaaa aggactgcaa ggctgggcag
     2041 gctggtatga ggacaacacc cagcaagccg atccgaaggc tcaggttgat agcatccgcg
     2101 cggcggcggc ggccgccaag caagccgatg ttgctatcgt tgtcgtcggc gagacggaaa
     2161 gcaccaaccg cgaagcctgg tcggagcagc atctcggcga tcgcgattcg ctcgatcttc
     2221 tgggcgcaca ggatcaactg atccaagcag tcgcggagac cggagttccc accgtcgttt
     2281 tcctgatcaa cggccgtccc ctttccatca actacgcggc ggagcacgtg ccagcgattc
     2341 tcgagggatg gtatctgggc caggagggcg gaactgctgc ggcacaggtt ctcttcggcg
     2401 acgtcaatcc cggcggaaag ctgccgatca cgttcccgcg ttccgttggc cagcttcccg
     2461 ctttctataa tcacaagcct tcgcgcaatc ggagctatat ctttacctcg cgcgagccac
     2521 tcttcccgtt cggttttggc ctcagctaca cttcgtttcg ctttgacaat ctgcgcgtcg
     2581 aaccggcgga gatcgcgccc ggtcaatcgg caactgtaag cgtggaggtc acaaataccg
     2641 gtgaccgcga gggagacgag gtaccccagc tctatattca ccagcgcgtg ggcaccgtga
     2701 cgcgcccggt caaggaactt cgcggcttcc agcgcttgcg gctgagaccc ggcgagaagg
     2761 ccaccgtgga attccggctt gcgccggagt cgctttcgat gctggacgaa cacatggacc
     2821 gcgtggtcca gtcaggcatg ttcgaacttt tcgtgggatc aagctcggcg caaaccagtt
     2881 cagtttccct gcgcgtggct cagcgttagc gcgtctcgcc gcaggcaagg ccgtgcgttt
     2941 taccaaaatt tgtcttcctt attcgttcaa ggcgggccgg aggacggcga tgacaaaatg
     3001 cgcaaaattc gaagcgccat cgtccagttg gagccaacgt acgacacagt gctcgcccag
     3061 atattttgca acgcgaggtt caggaattcc caggaactgt gcgagctgcg gcttgatcga
     3121 atccactccg cggcgactcg agccgccacg caacaagttg ccgatggcga tgcgcagcgt
     3181 ctggcaagct tccacgtagt aatagcttgt caggtccgaa tccgtcagga caaatacgcc
     3241 cggtcctgcc ggggcgtgat ccgcttcttc caatttggcg gacgggcagg tcatcagtct
     3301 gcgaaattgc atttccaata gagaaatccg ggaagagagg ccgggatcgc catagcgtcc
     3361 cttcacgccc gttccgcccg gctccccaat cggctcgatg ggcagggtgg gaatcgcgcg
     3421 gctgtgcgtc actcgaggag gagtcggctc ggccgattcc tcgaagacgg caggaggcaa
     3481 tccgcgtcgt ggcgcaggcg cttccgcagc gtgctgaccg ggagtaactg ctgcttcctt
     3541 cccgttgctg cccttgcgac ggttccggcc cccgcggcgt ccccgtcggc ggccttttcg
     3601 ttccggctcg gaagaagctg gcgcgttcat ggaattatcc gctgcttgct ggatctcgct
     3661 ggtaaccgcc acggacgatt ccagcgcttc tggattctgt tcgaatggca agtctgcctc
     3721 cggcgtcgaa accgcaggcg gcgtcaaagc aatctcctcc gtcggcgggg cccccgcttc
     3781 aagtgggatc tcaccgggcg cgccgccaag ccggaccgca gcttcctgcg ccttaatttt
     3841 ccaatccgag cgacggaatc tttccgccgc cgcggaatac catttcgcgg catcggcatt
     3901 ctgcccggaa gtttcgaaaa acttcgcgag ctcgaacgct accatggcgt cacgcgtctt
     3961 ctggtaaaga ttcgaaagtt gtcccgcggg atccttgctg gtgcgcgcgc ggcggatgcg
     4021 ggcggtaatc tgtttccagt cggtcatctc ttgcttgcat tgtaacagcg gaacggctca
     4081 gcggccagca aactccgttc gcgctcggac ggcgcgactt tttcctttca aaacgtgttt
     4141 accgattgac ggcacgaagt ttactcagta ttcttccggt ggccctcttg gaggcgttta
     4201 atgatacgcc tgcgcgttga ctgtcttaca gtcaccagca gcaaagaccg cttagcggtc
     4261 accagcagca tgaacctctt cgcagtcacc agtcgcaatg actgcatttc gattacgaaa
     4321 agcattctcg ccttgttcgt ttcattctcg ctcctgacca cgcctgtctg ggccgctcct
     4381 tcctcttcgc tcggtattgt cgtctacgcc gatcgcgccc acgttggcgc tgcgcaggca
     4441 tccgttggcg cgaccgtttt tagtggtgac agaatcagca ccgaccagtc aggcagcgtc
     4501 caggtccgcg ccggtgccgc ccgcttgctc ctctccagtg ccagcagcgc caccttttct
     4561 caggatgacg ccagcccgac agcaacattg actttcggct ccgcgacttt ctcaaccgcc
     4621 agttcgaacg cttttgcctt gcacgtggct tctgccgtga ttcgcccgaa ctccaaccaa
     4681 cccaccatcg gacaagttac ggtcctgaat cccaaggaac taatcgtcaa gagcactcgc
     4741 gggtcactga gtatcgccgt cgaagatgac gtgcgcgaaa ttccggaagg cgccgcctat
     4801 cgcatcgtcc tcgaccccaa cgcggccgac ccgcaggggc cgcgcggcgc gggcaccaag
     4861 ggtcatggcg ggccgccgat caaagccgcc aagagccggt tcatctggta cgccatcgct
     4921 gtcactgccg tgattactta cttcgcgctc gacgaagtgt tcgaaagccc cgaccgcccg
     4981 taagcgcagc catccaatct gctcctgctc gcaggcctct tctctcgagc gggtggcacc
     5041 ggcgacaaat tctaaaacga ttctgggtgc cgcatacgcc ggtgtcgtct accccgaccc
     5101 ggtcggggcg tgttaagagt gaacttcatg tgcaacaggt gtgagatcgg caagggtttc
     5161 gtcgcccgcg aaccatgctc cgggacttgc ctgcattact atcgcgccag atactacgat
     5221 ccatcaacct tattttattc gtctctcgcc ttcaagtcgg cggtcaattc aatctctaca
     5281 agcgcatcct tcgggagacg cgacaccggc attgcctttg ccgtgtaact tcttcacgaa
     5341 gcctccggaa gccccggcct cccataactt tattcgcttt tctaaacaaa ccacactccc
     5401 gtcggccaga gtcattgcca ctacctctgc atgatcgcag gcttgccatt cgccataatc
     5461 actgtcattc agccgcggat ttcgcttggg tgctaactaa gctactctgc tggcccttca
     5521 tgaaagagct cggggtgcgg cggcccagaa acctcgatcg caccaatcaa gctgcgagcc
     5581 acacggacga gcgcatggtc cacaagcttg tacgatccgg gcactagcgg tttgaattcg
     5641 ataatggttg ctcctccggg cggcacgaca gtagtctgca cggaagtgag cggcggcgag
     5701 gtaaaagagc cctcgttata gacttttgta agtacctgac ctataacgtg aggcgaagaa
     5761 atcaggttcg gtccgcctac accgaagaaa atccgcaccg tctcgccgac ttttgcatgc
     5821 ataggatgct gagaggtgag ggctccgacg gccccattga acacgaaata ttccggccgt
     5881 tcgtccatca gtttggacag actcatcttc tggtgccctg tcccaccgat atcttcgacg
     5941 gtgtagattt ctccttgcat gacgtagaac tcgtggtcca cgcgcggcaa accttccgga
     6001 ggttcaacga cgatcagacc gtacattcca ttcgcaatgt gctgagcggc aagattcgtt
     6061 ccgcagtgat agacaaacac gccgggcaca atcgccttga acgtaaaagt cctgctttcg
     6121 ccttgcttcg cttctgtgag atccatcccc ccgccgggcc ccagagctgc gtgcaggtcg
     6181 atggaatggg aaacgagtcg gccatcgttt ttcagcgtca gctcgacggt gtcatcttgc
     6241 cggacgcgaa gcatcgggcc aggcactttg ccgttgaacg tccaatactc gtacgtcgca
     6301 tccaagacgg cgtccagttt cccttccagt tcttgagcga tcagcgaaac tcttacgatc
     6361 tgcggctgtc gctttccaac gggcggcggg acgtccgctg gatcgcgcac gatgtccacg
     6421 gcggtctgaa tcggagcctg ctctggcgtt tgtgcttgga ccgcttcggg tggcattgct
     6481 gcatgagaag agtcatgctg cgacggtttt tccggcccag cagatgaact ttgtccagaa
     6541 gcaaccggtg tgccgccgag cacagccagt gcagatgccc aaagcagaga actcttgagt
     6601 atgcgattcc ttgtgtttgt catggccact gtatcctttt cccttcgtct tgccggtccc
     6661 ataaagcgac gacgccattg tgcaaggcgg tcttttcggc tgctgtgaca agagccacta
     6721 gtatggacct ggatcaggcc gctccaagaa ctgaaatgcc gcagtttggg ccgcagcgca
     6781 gcaattcaag tagcaggctc agtccgggag ctcctgggta agtaattgtg gtatccccac
     6841 gaccaaaggc tggttgcatg catgactggg ttgcaggaga aacgtgccca gaatgttggt
     6901 tgcaaacagg gtgagcccag ccaattcgaa tagagccgaa actggaagca cggaccaggc
     6961 ccaggccgcg taaccctggt aggcgagcac ttcgcaggaa acccgcaacg tgcaaccaat
     7021 cgacaacacc agcaggccgg cgaacataag ttttgggctc cagagcaaac gcatcccggc
     7081 aaacgcaggc agaatccgct ggccaatcga caagaccatc accgcaatga atcccacggt
     7141 cagcgcatgg cgtgacgcac cccagatccc tccggaggta tcccagatcg ccgccgcgac
     7201 gccaagcaac gctgcgacga gaagccagcc atacgccatg cggatgaaga gggggaacgt
     7261 ccggtgcacg cctttgattt ttgctggctg ttcactgcgt tcaaacaccc tgatcgccgc
     7321 aatcgatagg cctgccccgg cgacgaacac ccacgtcgct atccaaaact tcccggcaac
     7381 tgccagtgcc acgccggtgg agttgaccag aaccgcgccg agcaacaaac gcggacgcaa
     7441 cggcttcaac ccaagaaata cgcgcatcca cttggcgctg aatccccaca caaatggcac
     7501 caggaatccc caacccatta gcgccaggta gcgctgatcg aaccggtgcg gaaatgcggg
     7561 actggcgccg cggaaggaaa cgtaaaagct ccccgccacg
//
LOCUS       DASYVO010000304         2401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_247930, whole genome shotgun sequence.
ACCESSION   DASYVO010000304 DASYVO010000000
VERSION     DASYVO010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247930"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..1517)
                     /locus_tag="VGR03_10330"
     CDS             complement(237..1517)
                     /locus_tag="VGR03_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1994717.1"
                     /translation="MRANSPAAKANSCEQRVVRCAIYTRKSTEEGLTQPFNSLDAQRE
                     SAEAYISSQRHMGWTALERRYDDGGHTGANLERPALRALMEDIGSGQVDCVVVYKVDP
                     LSRSLLDFARLIGVFEQRGVSLVSVTQQMNTTDSLGRLTLNILLSFAQFERELIAERT
                     RDKMRAARRKGKWLGGNPVLGYDVDPQGGGLKTNPAEAEQVRFIFERIAAGKSGDEVL
                     RGLFRRGWTTKTWVSRAGRGHAGRAFQESDLIRLVSNVTYMGKLRDGDQIYPGEHAAI
                     IDCDLWERANAAVNGAPNCAPVAPASRSQLTTPELRAGVPVSPSVPRISRLLALALKM
                     EQMIEDGTVTKYSDLAHLGQVSAARITQIMNLLHLAPDIQEQILSPDIPHLELRESAV
                     RKLSTVVLWSEQRNRWQDLLAAASAQEGPILRLA"
     gene            complement(1504..2001)
                     /locus_tag="VGR03_10335"
     CDS             complement(1504..2001)
                     /locus_tag="VGR03_10335"
                     /inference="COORDINATES: protein motif:HMM:NF022598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2924 domain-containing protein"
                     /protein_id="HEV1994718.1"
                     /translation="MDLQLNEDIDRLRQMTTAQLRFRYHELFGHASHSNHKGYLFRRL
                     AWRLQSLADGGLSERARQYARQITEDADLRLCPPTKPMGIPSVRIAAARRADPRLPAP
                     GTQLVKRYKEDTITVTVLEDGFQHGERVYKSLSAIARQVTGTQWNGYSFFRLSSPKGH
                     RHESQ"
BASE COUNT          472 a          754 c          658 g          517 t
ORIGIN      
        1 agattagaga gctcggtgct gtctgcggaa gaaaagtatg gcccgcgcga gcctcaccgc
       61 agagttctcg gcggctttct cgaagctcat cctcccgatt tgcgcatcca acgccgccgc
      121 ggaatcttct tccggcagta ggttccaacg gccaaactcg tttaggtctg cgctcctatc
      181 gacatgcgcc gggcggtcga agtgattctg tggttcggcg ccgcccgtcg atggcctcaa
      241 gcgagacgca agattggacc ttcctgagcg gaagcggccg ccagcaaatc ctgccaacga
      301 tttctttgct cgctccagag tacgaccgta ctcagcttcc gcacggccga ctcgcgcagc
      361 tcaagatgag gaatgtcagg tgacaagatc tgctcctgaa tgtcgggcgc caggtgcaac
      421 aggttcatga tctgcgtgat gcgtgcggcc gagacttggc ccaggtgagc gagatcgcta
      481 tacttggtta ccgtgccatc ctcaatcatc tgttccatct tcagcgccag cgccaacaat
      541 cggctaatcc gcggcactga gggtgacacg ggaactccgg ccctcagttc cggtgttgta
      601 agttgagatc ggcttgccgg cgccactgga gcacaattcg gtgcaccatt gactgctgcg
      661 ttcgcccgct cccacagatc acaatcgatg atggccgcgt gctctcctgg atagatctgg
      721 tccccatctc gtagcttccc catatacgtc acattgctga cgagccgaat caaatcggat
      781 tcctggaatg cccggcccgc atgtcctcgg ccggctctgc taacccacgt cttcgtagtc
      841 cagccccgcc ggaatagtcc gcggagcacc tcgtcaccgc tttttcctgc agcgatccgc
      901 tcaaagataa atcgaacttg ctccgcctcg gccgggttgg tcttcagacc accaccctgc
      961 gggtccacat catacccgag gactgggttg ccacccagcc attttccctt ccggcgcgcc
     1021 gcccgcatct tgtcccgcgt ccgctcggcg atcagctctc gctcgaattg cgcaaacgat
     1081 agcagaatat tgagtgtcag ccgccctaga gaatcggtcg tattcatctg ctgcgtgacc
     1141 gacaccagac tgactccccg ctgctcgaag actccgatca gccttgcgaa atcgagcaag
     1201 ctccggctca gcggatccac tttataaacc actacgcaat cgacctggcc cgacccgatg
     1261 tcctccatca gcgcccgcag cgccggccgt tccaggtttg ctcccgtgtg gcctccgtcg
     1321 tcataccgcc gctccaacgc tgtccagccc atgtgtcgtt gactggagat gtaagcctcg
     1381 gcggactcgc gttgcgcatc cagcgaattg aaaggctgcg tgaggccttc ttcggtggac
     1441 ttgcgcgtgt aaatggcgca acgcacgacc cgctgctcac acgagttagc cttggcggcc
     1501 gggctattgg ctctcatggc gatgtccctt aggcgaactc aagcgaaaga acgaatatcc
     1561 gttccactgc gttccggtca cctggcgagc gatagcgctg agcgacttgt agacgcgctc
     1621 tccgtgttga aagccgtctt ccaacacggt gacggtgatc gtatcctctt tatagcgttt
     1681 gaccagttga gtgccaggag ccggcaggcg gggatcggct cgccgtgcag cagcgattct
     1741 aaccgatgga atgcccatgg gcttcgtggg cggacagagg cgtaagtcgg catcctcggt
     1801 gatttgtcgc gcgtattggc gggcgcgctc cgacaggccg ccgtcggcca gactctgcag
     1861 gcgccacgcg aggcgacgga acaggtaacc tttgtgatta gaatgactgg cgtgtccgaa
     1921 cagttcgtga tacctgaacc gtaattgcgc cgtggtcatc tgccgtaatc gatcgatatc
     1981 ctcgttcagt tgtaaatcca tgcgtttctc cgtttctctc ggaaacagca gccctactct
     2041 caggctgcgt taaaccggca cctgtcatgt acgctctttg ccggtagcat tgcaagcgcc
     2101 cgaagataac gcatccgcga cgcggcattg acgcgaaata acgcgaaaat aacgctacgg
     2161 ataacgcgag aataacgcgg gggaatgcgg gttgcgcccg ggaaccatgc ctgtatgcta
     2221 ctgaatgtag gtcgttccgc cccttctgcg caagatgagc atcgcgttaa aaggaacgca
     2281 ctcgcgttat ttaacgcaag taaggctttt tgagcgtccc gtttctctcg aaaacgacac
     2341 ccttcagacg ctcttcgccg cctagtcttc ggggacccaa gcgaaaaggt ccaccctccc
     2401 g
//
LOCUS       DASYVO010000305         5971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_145902, whole genome shotgun sequence.
ACCESSION   DASYVO010000305 DASYVO010000000
VERSION     DASYVO010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145902"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..803)
                     /locus_tag="VGR03_10340"
     CDS             complement(135..803)
                     /locus_tag="VGR03_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994719.1"
                     /translation="MPETPSKTAARVPSSFLEYRAFYKERFFDIQNQYSQVASSVFRA
                     YKDWNIRLENITYKQNPLNFGEISLSVAVPGDRIVLNVGLAGVSVNVSNPDWEEAERI
                     VKAVDIGIAAVKTATGLEIGSQRAVLAMHLQAISGTLSDRVLDLVRIDANLLPGKGTR
                     GFGVSVYKDDLTWLVDMSAFFQGALFVKMDRLFGPDVSLGDIGAQLNQDEQKLFEMLR
                     LEVE"
     gene            1939..3363
                     /gene="leuC"
                     /locus_tag="VGR03_10345"
     CDS             1939..3363
                     /gene="leuC"
                     /locus_tag="VGR03_10345"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEV1994720.1"
                     /translation="MPAAKTLYEKIWDSHIVYEEPGQPAVIYIDRHLIHEGTSPQAFA
                     GLRAEGRKVRRPDLTFAVMDHSVPTTDRTLPILDQDAKLQFQALEKNCAESGVRLFDM
                     NSRHQGIVHIIGPELGITQPGQTIVCGDSHTSTHGAFGTLAFGIGTSEVEHVLATQCL
                     VQSRSKTLHVLVHGKRPKGVTAKDIILAIIGKIGIGGGTGHVIEYAGEAIRTLSMEGR
                     MTVCNMTIEGGARAGMVAPDDTTFAYLEGRPFVPRGKAFQEAVEGWKQLSADPGARFD
                     MTVELNASEIAPQVTWGTNPGMVTSVTSCVPDPHAFSDSNDRKATESALKYMGLKPGT
                     PITDIRLDRIFIGSCTNSRLEDLRAAAQLVAGKRIAGSIKQALVVPGSRGIKTQAEKE
                     GLDRIFRDAGFEWRDAGCSMCIGMNADVLPPHERCASTSNRNFEGRQGKDGRTHLVSP
                     LMAAAAAIAGHFVDVRSLDVPSVE"
     gene            3410..4051
                     /gene="leuD"
                     /locus_tag="VGR03_10350"
     CDS             3410..4051
                     /gene="leuD"
                     /locus_tag="VGR03_10350"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV1994721.1"
                     /translation="MQPFSTHSGLVAPLDRVNVDTDQMVPKQFLKALTRQGFGRILFY
                     DWRYLPGEKPNPDFVLNFPRYKGASVLLARANFGCGSSREHAPWGVREYGFRVIIAPS
                     YADIFYNNCFKNGILPATLSEEQVEELFQRTESEADYSLTVSLPDQTIRDNQGHAYRF
                     EILPHRKEVLLKGLDEIGITLQHADAIAAYEKARSTTATMYEPLDVKNYSNRD"
     gene            complement(4065..4988)
                     /locus_tag="VGR03_10355"
     CDS             complement(4065..4988)
                     /locus_tag="VGR03_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV1994722.1"
                     /translation="MRNNRQIKADLALALTCIVWGATFVVVKDAVNRASVFLFLAVRF
                     SLAAILMAAIQRKTLSRLRRKEVFAGAFLGLLMFSGYAFQTSGIVFTTPAKSGFVTGS
                     SVVLVPLLLAIFWRRRLGLWEYLGAGGAFAGLYFLTAPGGRLAELNVGDILTFVAAAL
                     YAFHIIFAGQYTRAHSVAALSFLQVAATAILAIGLCGLTAITGWQRPRFEVSWQLAAG
                     ILITAVFATAIAFTVQLWAQRYTTASHTAIIFTFESVSAAITSYLVIGERLRGRALAG
                     AALVVAGILFAELKGPAPAAPESPEPAAGSA"
     gene            complement(5095..>5971)
                     /locus_tag="VGR03_10360"
     CDS             complement(5095..>5971)
                     /locus_tag="VGR03_10360"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV1994723.1"
                     /translation="DGKLTARITEWLPFTAPLERLVEMRREGGTTDPWLRTGAVKIVT
                     DGAVGTHTAAMLAPYSDAPSTSGILIMEPRKLRELAIERDRAGFQLAFHAIGDRANRV
                     ALDVFEAVANANGPRDRRGRIEHAQVVALDDLPRFAKLNIIASMQPSHETNDLRWAEQ
                     RLGPDRIKGAYAWATLSKSGARLAFGTDYDVEPISPFRGLYACVTRELPEGGPAGGWQ
                     PQEKISLADCIRAYTSGSAYAEFAEGKKGELKAGEFADFIVLSGDLTKLPPSEYTKLH
                     VLRTVVGGRTVYQGP"
BASE COUNT         1273 a         1905 c         1587 g         1206 t
ORIGIN      
        1 ctcaggcggc acaagccctt gccgctcaaa attggccagc cagccgcctt ccgaaaacaa
       61 gtcagcagcg tcttgagcaa tttcgaaact ccgctcctgc ttttcaagac ccaatagctg
      121 tagcgtcgcc atgctcactc aacctctaag cgaagcattt caaaaagctt ctgctcatct
      181 tgattcaatt gagctccgat atctcccaaa gaaacatcag gcccgaacag tctgtccatt
      241 ttaacgaata atgcgccctg gaagaatgcc gacatgtcta ctagccaagt caaatcatcc
      301 ttgtaaacag aaacaccaaa cccacgagtc cctttgccag gcaagagatt cgcgtcgatt
      361 cgaactaagt ctaaaacacg atcagacaga gtaccggata ttgcctgcaa atgcatcgca
      421 aggactgccc gttgtgagcc aatttcgaga cccgtcgcag tcttcacagc ggcaatgccg
      481 atatccactg ccttcactat tctttctgct tcctcccaat ctggattgct cacattgact
      541 gaaactcctg ccaagcccac gtttaataca attctgtcgc ccggaactgc aaccgaaaga
      601 gaaatttcgc cgaaatttaa gggattttgc ttgtacgtga tattttcgag acgaatgttc
      661 caatccttgt acgctcgaaa tactgagctg gcgacttgag aatactggtt ttgaatgtca
      721 aagaatcgct ctttataaaa tgcccgatac tcgaggaaag aagaaggtac ccgagctgcg
      781 gtcttagatg gtgtctcagg catagcactt gggtgcgtag acggcaacca ggctctagta
      841 tgatgtaaat ggcttctgta aacaattccg tctgctgaaa taggaaacaa ccgtattggg
      901 ggagtttatt accccgcggc ttataattgc tccattgtgt agagtgccct tgggaaacgt
      961 aggattcttt ggttagatag aagcctaccg cgccatttcg cctcctgtct ggctacctcc
     1021 gggttcaccc agtctcctat cccctttccc gtcagccact tacactagtt ccttgacaat
     1081 gagttaactc ttgcggtatt ataccccgtg agtttcgcgc tacaggccgc cttaccgctt
     1141 ccgctcgtca catcatcgct tccctgcgac ttcaccttcg cctctgcgtt cctccgcgga
     1201 tccttctccg tgtcctccgc gctaagtccg ccgctctttt cgcgtctttc ttggctcgcc
     1261 actcaccact cgtcactcgc cactaacttc tttggaatca acacttgtaa aagcgagcat
     1321 ccgattagga tgcgagtccc gagaagcatc gcgacgaggg atctctcttc aaccgcgaca
     1381 ttcactaacc ctcgcacaat cgctcgtaac tcatttagaa tcaacacttg taaatctgta
     1441 tcaaaacaaa gaacttcaac tccctttgga atcaacactt acaaaaaaca ggggggaggg
     1501 ggagtatctc ttctgttaac ggtcctggta ctccgctcct gccggaaaag ggccacctca
     1561 catccggtca ggtcttgcgc ctcctctacc tcatttactc ccactatttc atctaactcc
     1621 tttttcccct tcgtgttcct cactctttct cactcttctc ccgcaagtct tttgtttgcc
     1681 actcatacgc aaatcaatcc gggggtgggg ggtgttgtgt tagcgattat cgccgtctcg
     1741 catccgtact ccgtgacctc tgtgccctct gtggtgaatt ccctttccgc tcctcgcgga
     1801 agggacctct cttcgtatcc ggtcaggtgt tttgcctccc ctacctcatt tacctcctct
     1861 gcttccttta cctccagcgc tccgccagcc acccctcacc agccactttc ttccgtgcta
     1921 gaatcccacc ggtcacccat gccagccgcc aaaaccctct acgagaagat ttgggactcc
     1981 cacatcgtct acgaagagcc cggccagccc gccgtcatct acatcgaccg tcacctgatc
     2041 cacgaaggca cttccccgca ggccttcgcc ggactccgcg ccgaaggccg caaagtccgc
     2101 cgccccgacc tcactttcgc ggtcatggac cattccgtgc ccaccaccga ccgcacgctt
     2161 cccatcctcg accaggacgc caagctccaa ttccaggccc tcgagaagaa ctgcgccgag
     2221 tccggcgtgc gcctgtttga catgaacagc cgccaccagg gcatcgtgca catcatcggc
     2281 ccggagctcg gcatcaccca gcccggccag accatcgtct gcggcgacag ccacacctcg
     2341 acccacggcg cgttcggcac gctcgccttc ggcatcggca ccagcgaagt cgagcacgtc
     2401 ctcgccacgc aatgcctggt gcagtcgcgc tcgaagacgc ttcacgtcct cgtgcacggc
     2461 aagcgcccca aaggcgtcac cgccaaagac atcatcttgg ccatcatcgg aaagatcggg
     2521 attggaggcg gtacaggcca cgtcatcgaa tacgccggcg aagccatccg cactctttcg
     2581 atggagggcc gcatgaccgt ctgcaacatg acgattgaag gcggcgcccg ggccggcatg
     2641 gtcgcgcccg acgacacgac cttcgcctat ctcgaaggcc ggccgtttgt gccgcgcggg
     2701 aaggcgtttc aggaagcggt cgagggctgg aagcagcttt ccgctgatcc cggagccagg
     2761 ttcgacatga ccgtcgagct gaatgcatct gagatcgccc cgcaggttac ctggggcacg
     2821 aaccccggca tggtgacctc cgtcacgagc tgcgtcccgg atccgcacgc cttctccgac
     2881 tccaacgacc gcaaggccac cgagagcgcg ctgaaatata tgggcctgaa acccggcacg
     2941 ccgatcaccg atattcgatt ggatcgcatc ttcatcggct cgtgcacgaa ctcgcgcctc
     3001 gaggatctgc gcgccgcggc gcagcttgtc gccggcaagc gcattgccgg ttccatcaag
     3061 caggcgctcg tcgttcccgg ctcgcgcggc atcaagaccc aggccgaaaa agagggcctg
     3121 gacagaatct tccgcgatgc gggcttcgag tggcgcgacg ccggctgcag catgtgcatc
     3181 ggcatgaacg ccgacgtgct cccgccgcac gagcgctgcg ccagcacgtc aaatcgcaat
     3241 tttgagggcc gccagggcaa ggacggccgg acgcatcttg tcagcccgct gatggccgcc
     3301 gcggccgcca tcgccgggca tttcgtggat gtgcgttcac tcgacgttcc ttccgtcgaa
     3361 tgaacgtagt tttgtgctaa gtgttctgat tttctgggag ctttgtcgaa tgcagccttt
     3421 ctcgacgcat agcggcctgg tcgctccgct cgaccgcgtc aacgtggaca ccgatcaaat
     3481 ggtgcccaaa caatttctga aagccctcac gcgccagggc ttcggcagaa ttcttttcta
     3541 cgactggcgc tacctgcccg gcgagaaacc caacccggat tttgttctga actttccccg
     3601 ctacaagggc gcatccgttc tccttgcccg tgcgaatttc ggctgtggct cgagccgcga
     3661 gcacgcaccc tggggcgtca gggaatacgg cttccgcgtg atcattgccc cgagctacgc
     3721 cgacatcttc tacaacaact gcttcaagaa cggcatcctc cccgccacgc tctccgagga
     3781 gcaagtggaa gagcttttcc agcgcaccga gagtgaagcg gattattcgc ttacggtgag
     3841 tcttcccgac caaaccattc gagacaatca aggccacgct taccggttcg agatacttcc
     3901 ccaccgcaag gaagttctgc tcaaaggcct cgacgaaatc ggcatcaccc tgcagcatgc
     3961 cgacgcgatt gccgcttatg aaaaagcccg cagcaccacc gcgacgatgt acgagcctct
     4021 ggatgtaaaa aactactcta atcgagacta gttcgccgct tcgctcaagc ggaaccagca
     4081 gcgggttccg gcgattccgg cgccgccggg gccggccctt tcaactccgc aaacaaaatt
     4141 ccggccacca ccagcgcggc tcccgcaagc gcccgcccgc gcagccgctc accaatcacc
     4201 agataagaag tgatcgcggc ggacaccgac tcgaacgtaa agataatcgc cgtgtggctt
     4261 gccgtcgtgt agcgttgcgc ccaaagctga accgtgaacg caatcgccgt cgcgaacacc
     4321 gccgtaatca ggatgcccgc ggcgagctgc cagctcacct cgaaccgcgg gcgctgccac
     4381 ccggtgatcg ccgtcagacc gcacaagccg atcgccagga tggccgtggc ggccacctgc
     4441 aggaagctca gcgccgcgac ggaatgcgcc cgcgtgtatt gtccggcgaa gatgatgtga
     4501 aacgcataca gcgcggcggc gacgaaagtc agaatgtcgc cgacgttcag ctccgcgagt
     4561 cgccctccgg gggcggtgag aaaatacaat ccagcgaacg ccccaccggc ccccaagtat
     4621 tcccaaaggc ccaaccttcg ccgccagaaa atcgccagca acaacggcac gagtacgacg
     4681 ctggacccgg ttacaaatcc agatttcgcg ggagtcgtga agacaatccc ggaagtttgg
     4741 aacgcataac cagaaaacat caggagcccg aggaaagctc cggcgaaaac ttccttgcgg
     4801 cgaagacggg acaaagtttt tcgctggatc gcggccatga ggatcgcagc cagggaaaag
     4861 cgcacagcga ggaaaagaaa aacagaggcg cgattgacgg cgtccttgac gacgacaaac
     4921 gtggcgcccc aaacgatgca agtgagcgcg agagcgaggt ccgccttgat ctgccggttg
     4981 ttacgcaccg tgattttccg accgatggag ttccctatcg taatcgcaat cggagctgca
     5041 aagaaagaat tgcgtgggaa gtggttcgaa tcacggcgaa cgacaggccg gcgttcaagg
     5101 accttgatag acggtgcgac cgccgacgac ggtgcgcagg acgtggagct tcgtgtattc
     5161 agagggaggc aacttcgtca ggtcgccgga gagaacgatg aagtccgcga actcgcctgc
     5221 tttcaattcg cccttcttcc cttccgcgaa ttcggcataa gcggagccgg aggtgtaggc
     5281 acgaatgcaa tcggcgagag agattttctc ctgcggctgc cagccgcccg cggggccgcc
     5341 ttccggaagc tcgcgcgtga cgcacgcata aagtccgcgg aacgggctga tcggttcgac
     5401 gtcgtaatcc gtaccgaaag cgagccgcgc gccggatttc gaaagcgtgg cccaggcgta
     5461 ggcgcctttg atgcggtcag ggccgagccg ctgttcggcc caccgtaggt cgtttgtttc
     5521 gtgcgagggt tgcatggagg cgatgatgtt gagttttgcg aagcgcggca agtcatcaag
     5581 ggcgacaacc tgggcatgtt cgatgcggcc gcggcggtcg cgggggccgt tggcattggc
     5641 gacagcctcg aacacatcca gggcaacacg attggcgcgg tccccgatgg cgtgaaacgc
     5701 caactggaag cccgcgcggt cgcgctcgat ggccagctcg cgcaattttc gcggctccat
     5761 gatcaatatt ccgctggtgc tgggcgcgtc ggagtaggga gcgagcatcg ccgcggtgtg
     5821 cgtaccgaca gcgccgtccg tgactatttt cacggcgccc gttcggagcc agggatcggt
     5881 tgtgcctcct tcgcggcgca tctcgacgag gcgttcgagg ggcgcggtaa acggcaacca
     5941 ctcggtgatg cgggcggtga gcttgccgtc c
//
LOCUS       DASYVO010000306         5831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_165939, whole genome shotgun sequence.
ACCESSION   DASYVO010000306 DASYVO010000000
VERSION     DASYVO010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165939"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="VGR03_10365"
     CDS             <1..794
                     /locus_tag="VGR03_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEV1994724.1"
                     /translation="TNVKDVAAILQVIAGRDPHDSTSTTAPVPDYRAELGKPVKGLRL
                     GIPKEYFGKGMDAGVRKKIETGIEIFKKLGCELVEIRMPHTDYAIATYYIIATAEASS
                     NLARYDGVRYGLRVDNDSLLAMYRKTRGAGFGAEVKRRIVLGTYVLSAGYYDAYYLKG
                     QKVRALIAQDFRDAFAKVDAILTPTSPVPPFKLGERTDDPLQMYLADIYTVTGSLAGV
                     PGISVPCGKIDGRLPVGLQIFGAAFGEARVLQLAHAFEQAGGAAV"
     gene            822..1361
                     /locus_tag="VGR03_10370"
     CDS             822..1361
                     /locus_tag="VGR03_10370"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV1994725.1"
                     /translation="MPSLATIIENLAHARSELLRAADVVPVDQWKTRPAERRWSAGEL
                     IGHLSAIERAILGRADKLLQKPPKPVPFYKRFHVPMMIVEARVIRRKAPIALEPQMVC
                     EKEEMLAELRGVRERTLAFIDETRGRDLSKYNMAHPFLGTLNAYEWLQFIASHEIRHM
                     KQMREIGGSLPKTVTSLHK"
     gene            1596..1814
                     /locus_tag="VGR03_10375"
     CDS             1596..1814
                     /locus_tag="VGR03_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994726.1"
                     /translation="MVTVKSSQKIFTDSEVASLTGICVEHVHNFAKSRHLGFIARAAE
                     AAGISADQWLFTLSDLMVLVTLFPKCTH"
     gene            2237..2995
                     /locus_tag="VGR03_10380"
     CDS             2237..2995
                     /locus_tag="VGR03_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994727.1"
                     /translation="MSAERGDWEEIPAAMVDWDATAKAAAAEQAEEDALAKKLHAQEQ
                     AQKIETVMDVDASLQVAPGVFLPPGEGMFLIDGKSVKPLEQVGSQIKTDKKQYLKQVL
                     SPIPIVPSKQNVLIPGTRASIRVTDGQAEFYLREAPPDPDRTTAIVKSSRPGESGPEV
                     ELVRATVKGNKRQLESIKSLFGEQMEENRNTISIQRWEIAPTVYRFTLSEPLPPGEYA
                     LAEILQDGMNLFVWDFGVDPATSAKPTTPPKKKN"
     gene            complement(3081..5744)
                     /locus_tag="VGR03_10385"
     CDS             complement(3081..5744)
                     /locus_tag="VGR03_10385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV1994728.1"
                     /translation="MLDEKQGARSTTGNSSPGASIRSVISSRFEQFEKRQNELWRLTF
                     IVLLLLGVAYVLTSWDSIRSFWHRFEAIAFSAGLFVLIVLFTVYMWNKNRELSELRGL
                     MRGLEQHDAAPPSDNQLDQLFAIISRSQQGYRDLIDSFDDVLLALTLDGQIRAVNRSF
                     SDLVGTPFQQIIGKPITDFLQEGSGEGADLLRRAMPRLMERRQWSGVVQVRLKGQGTL
                     FYFDCVAHAMMRDGEIHGITVLGRDVSALRINEARFTELFESLQEGIYITTPDGAILD
                     VNPALVRMLGYDSKEEVLKRRVPEIFVDHAERKTVKDQVERQPMIQGREITLIRKDGT
                     SIVCLNTAAAVRDNAGRVVRYQGALMDVTERREMERRLHQQQEFARRLVDSFPDLILV
                     LDTAAHYTFVSPRCKEVLGYDVEDTQQMEFGGRTHPEDLPAVLSLYKDIIAGKQTFAS
                     LEIRVRHKLGDWRRIRFNFSPLSDEKGSIEGVVLSGRDVTDLKRLEEQLIQAEKLAAM
                     GQMLAGVAHELNNPLTAILGVTELLREREGTDDSTKRQLELTHRQARRAARIVQNLLE
                     FSRPASPQKKPLDLNNLLDRTLQLQEHSLRRNNIEVDFHPQADLPGVIGDANQLIQVF
                     LNLVTNAEQAIREVRDTGRIQIRAGRNGNQLSITVQDDGVGIRPEALPRIFDPFYTTK
                     RPGGGTGLGLSICMSIIREHGGNIEAETLPAGGSAFTIYLPAAPGQQPEALPVPSETG
                     STSSEAVRPMADHLRGRAVLVLEDEESLRMLLQEGLSAQGLRVDCAATTGEALDHIQR
                     SSYDVFLCDLHLSAASIVDGREAASRILKAARAQRPAVIYMTGDLVESTQGTAGRGEP
                     VCLQKPFRISDVLALLRDVLSEATAETRHTL"
BASE COUNT         1304 a         1559 c         1838 g         1130 t
ORIGIN      
        1 cgaccaatgt aaaagacgtc gcggcaatcc tgcaagtcat cgccgggcgc gacccgcacg
       61 attcgacttc gacgacggcg ccggttcccg attaccgggc ggaacttggg aaacccgtca
      121 aggggctgcg actgggcatc cccaaggagt acttcggcaa aggcatggat gcgggagtac
      181 ggaaaaagat cgaaaccggc atcgaaattt tcaagaaact aggatgcgaa ctggtcgaga
      241 ttcgcatgcc gcatacggac tacgccatcg cgacctacta catcatcgcg acggcggaag
      301 cgagttccaa cctggcccgg tacgacggcg tgcgctatgg attgcgcgtt gataacgatt
      361 ccctgctcgc gatgtatcga aaaacgcgag gtgcgggatt tggagcggag gtaaaacgcc
      421 gcatcgtgct cggcacgtac gtgctttcgg cgggttatta cgacgcctac tacttgaagg
      481 ggcagaaggt tcgtgcgctg atcgcccagg atttccgcga tgcatttgcg aaagtggacg
      541 cgatcctgac gccaacttcc cctgttcctc cattcaaatt gggggagcgc acggacgacc
      601 cgctccagat gtatcttgct gatatatata cagttacggg atcacttgcg ggggtcccgg
      661 ggatctcggt gccctgcggc aaaattgacg ggaggctgcc ggtaggtctg caaatttttg
      721 gagccgcttt tggagaggcg cgcgtcttgc aattggccca tgctttcgag caggccggag
      781 gagccgctgt ctgagtctct tgcgcgtgtg tggatgaaac cgtgccatca cttgcgacca
      841 tcatcgagaa tctggctcat gcgcgaagcg aactcttgcg tgccgcggat gtcgtccccg
      901 tggaccaatg gaaaacgagg cctgccgaaa ggcgatggtc ggctggcgaa ctgatcgggc
      961 accttagcgc gatcgagagg gctatcctcg gtcgtgccga caagctgctg caaaagcccc
     1021 cgaaaccggt gcccttctac aaacgctttc atgtccccat gatgatcgtc gaagcacgcg
     1081 tgatccggcg gaaggcgccg atcgcgctgg aaccgcagat ggtctgcgaa aaggaagaga
     1141 tgctagcgga attacgtgga gtgcgggagc gcacgctggc gttcatcgac gagacgaggg
     1201 gccgcgacct aagtaagtac aacatggcgc acccgttcct ggggactctg aacgcgtatg
     1261 agtggcttca attcatcgcc tcgcatgaga tacggcacat gaagcagatg cgagaaattg
     1321 gcgggtccct accgaaaacc gtaacaagtt tgcataagta gaactttttt accacttggc
     1381 tggcctttgc tttcccttcg taccagctct ccgaactgtc aaaaagtgtg gaaaaagcca
     1441 gaatttactg ggctttaggg ggaaagtact agacggatgg tttttgcctg gcagaacgga
     1501 acgactattg ctcaggaata cgtgcagcgc ttacgatgcc tacagcatca aaggatattg
     1561 aaggagaagt tggagcggag cagaggagcc gggccatggt taccgtgaag tccagccaaa
     1621 aaatcttcac cgattcagag gtcgccagct tgacgggtat ttgcgtcgag cacgtgcaca
     1681 attttgcgaa gagccgtcac ttgggtttca tcgcgcgggc agcggaagcg gctggcatca
     1741 gcgccgatca atggctgttc acactttcgg atctgatggt tctggtcact ctgtttccga
     1801 aatgcacgca ctaaagcatt aagatcaaat tggtttttgc atggctccct ggcttggccg
     1861 gggagccatt tttgtttcgg gggcgtccaa gcggcaggtt gcgggtccgg aaggcaaggg
     1921 ggtatgctcg cgacagaggc aaaccgggcc ggctcgcatg catcgcacac cacaggaaag
     1981 gccgatggga tcacccagaa tgctcgtgct agtctattgc gctttcctgg caagttgggc
     2041 cggcacgccc ggttactccc cctcggcgag cggtacgtca gccagtcaag aacaaccacc
     2101 cgcagagaag caagcccgaa gcaaggacgg gcaggttgga gtgcctgcct tgccgcgggg
     2161 caagaagctc gtcctgaagg acggtacctt tcaactggtt cgggattatc agcggaacgg
     2221 agagcgggtc cgctatatga gcgcagagcg gggcgattgg gaagagatcc ccgccgcgat
     2281 ggtggattgg gacgcgacgg caaaagcggc ggcggcggaa caggccgagg aggatgcgct
     2341 ggccaagaag ctccacgcgc aggagcaagc ccagaaaatt gagacggtga tggatgtgga
     2401 cgcgagcctg caagtggccc cgggtgtctt cctaccgccc ggagagggga tgtttctcat
     2461 cgacggaaag tccgtgaagc ctctggagca ggtgggctcg cagatcaaaa cggacaaaaa
     2521 gcagtatctg aagcaggtac tttcgcccat tcccattgtt cccagcaaac aaaacgtgct
     2581 aattcccgga accagggcca gcatccgggt gaccgatggg caagcggagt tttacttgcg
     2641 cgaggcaccg ccggatccgg accggacgac ggcaatcgtg aaaagcagcc ggccgggtga
     2701 atccggtccg gaagtggagc tagtacgggc gaccgtgaag ggcaacaagc gacagttgga
     2761 atcgatcaag agtcttttcg gcgaacagat ggaggaaaac cgcaatacga tttcgatcca
     2821 acgatgggaa atcgcgccta ccgtgtatcg cttcactctg agcgagcccc tgccgccggg
     2881 cgaatacgcg ctggcagaga ttctgcagga tggaatgaac ctcttcgtct gggattttgg
     2941 ggtggacccc gcaacgagcg ccaagccaac aacgccgcca aaaaagaaaa actagttctg
     3001 caattaatgc acgcagggag cgttcggaaa cgagcgaatc ctgcgccctt caaaacgtgc
     3061 cgaaggaaac caggaaagaa tcaaagggta tggcgcgttt ccgccgtagc ttctgagaga
     3121 acatccctca agagagccag cacgtcagag atgcggaaag gcttttggag gcaaacaggc
     3181 tcgccgcggc ccgctgttcc ctgagtgctt tcaaccaaat cgccggtcat gtagatcacc
     3241 gcaggtcttt gggctcgcgc agccttgagg atgcggctgg cggcctcgcg gccatccacg
     3301 atgctggcgg cggaaagatg caggtcgcag agaaacacgt catagctgga gcgctggata
     3361 tgatcgagag cctctccggt ggtcgcggca caatcgacgc gcaaaccctg ggcagagagg
     3421 ccttcctgaa gaagcatccg gagactctct tcatcttcga gaacgagtac cgcgcggccc
     3481 ctgagatgat cggccattgg tcgaactgct tccgaggacg tgctcccggt ttccgaagga
     3541 acgggaagcg cctcgggctg ctggcccggc gcggcaggga gataaatggt gaaggccgag
     3601 ccgccggcag ggagtgtttc ggcttcgatg tttccgccat gttcgcgaat gatggacatg
     3661 cagatgctga gccccaggcc ggtgccgccg ccgggacgct tggtggtgta aaaggggtcg
     3721 aagatgcgcg gaagcgcttc ggggcggatg ccgacgccat catcctgaac ggtgatcgaa
     3781 agttgattgc cgttgcggcc ggcacgaatc tggatgcggc ccgtgtcgcg aacctcgcgg
     3841 atggcctgtt cggcgttggt gaccagattc agaaagacct gaatgagctg gttggcgtct
     3901 ccgatgacgc cgggaagatc ggcctgtgga tggaaatcca cctcgatatt gttgcggcgc
     3961 aaagagtgtt cctgcaactg gagcgtgcgg tcgagaaggt tgttgaggtc gaggggcttt
     4021 ttctgtgggg aagcggggcg tgagaattcg agaagattct ggacgatgcg cgcggcacgg
     4081 cgggcctggc ggtgcgtgag ctccagttgg cgcttggtgg agtcatcggt tccttcacgt
     4141 tcgcggagaa gctcggtaac gccgaggatg gctgtcaaag gattgttcag ctcatgggcg
     4201 acgccggcga gcatctgtcc catcgcggca agtttctccg cctggatgag ctgttcctcg
     4261 agacgcttga gatcggtaac atcgcgaccg gagagaacca cgccctcaat gctgcccttt
     4321 tcatcggata aaggactgaa gttgaaacga atccggcgcc agtctcccag cttgtggcgc
     4381 acgcgaattt ccagagaggc aaaggtctgc ttgccagcga tgatatcttt gtaaagcgac
     4441 aggacggccg gcaaatcctc cggatgggta cggccgccaa attccatctg ctgcgtgtct
     4501 tcaacgtcgt acccgagcac ctctttgcaa cgaggactga caaaggtgta gtgggccgcc
     4561 gtatcgagca ccaggatgag atcggggaaa ctgtcgacta ggcggcgggc aaattcctgc
     4621 tgctggtgga ggcggcgctc catctcgcgg cgctcggtga cgtccatgag cgcgccctga
     4681 tagcgaacca cacggccggc attgtcgcga acggcagcag ccgtgttcaa gcacacaata
     4741 gatgtgccat ccttgcgaat tagcgtgatc tcccgcccct gaatcatcgg ctgacgctcg
     4801 acctgatcct tgacggtctt tcgctctgca tggtcaacga agatttccgg aacccgtctt
     4861 ttcagcactt cttccttgga atcgtagccc agcatgcgca ccagcgcagg attgacgtcc
     4921 aaaatggcgc cgtcgggcgt ggtgatgtag atgccttcct ggagcgattc gaagagttcc
     4981 gtaaagcggg cttcgtttat gcggagcgcg gagacgtcgc gcccaagaac agtgatgccg
     5041 tggatttcgc cgtcgcgcat catggcatgc gcgacacaat cgaagtaaaa gagcgtccct
     5101 tgccctttca gccgcacctg gacgacaccg gaccattggc ggcgttccat gagccggggc
     5161 atcgcgcgcc gcaaaagatc tgccccctcc ccgctgcctt cctgcaagaa atcagtgatg
     5221 ggcttgccga taatttgctg aaagggcgtc ccgacgagat cggaaaaact gcggttgacg
     5281 gcgcgaatct ggccgtccag cgtaagggcg agcagaacgt cgtcgaagga gtcgatcagg
     5341 tcgcgatagc cctgctgaga ccgcgagatg atggcgaaca gctgatcgag ctgattgtcg
     5401 ctgggagggg ccgcatcgtg ctgctctagg ccgcgcatca ggccgcgcag ctctgagagc
     5461 tcgcgattct tgttccacat gtagacagtg aataggacga tcaaaacaaa cagacctgct
     5521 gagaatgcta tcgcttcgaa gcggtgccaa aaggaacgaa tggagtccca ggatgtcagg
     5581 acataagcta ctccgagcag aagaaggacg atgaaggtga gacgccagag ttcgttttgc
     5641 ctcttttcga actgctcgaa ccgcgaggaa atgacggaac gaatgctggc gcctggtgaa
     5701 gagtttccgg tggtgctgcg agcgccttgt ttttcgtcga gcataatggt aggcgggacc
     5761 ccgttagagc aggagtcacc cgccgacaag agatataccc cttattgtag gaattccaac
     5821 cggatttcag c
//
LOCUS       DASYVO010000307        10289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_36469, whole genome shotgun sequence.
ACCESSION   DASYVO010000307 DASYVO010000000
VERSION     DASYVO010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 10289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10289
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36469"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..2485
                     /locus_tag="VGR03_10390"
     CDS             119..2485
                     /locus_tag="VGR03_10390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV1994729.1"
                     /translation="MRGAKTIPLRTAPSVKGAGDNLHGASSCAEELMDLALDAAQSKN
                     LPDFLRQFSARAGRMLKANWCGVAVFQGEEADLYSSNSEPGKNTQTTRSLAKAILETA
                     YETENAIHVVNSALKEFPAADRADTSLETVLTRIDSGDGERLGCLCLLRKPQPLGDPD
                     LRLLRALASHAALSLVNFRRFSQLERSKRQWVEDIDAITDYIVVHDRSWRILRTNRTL
                     ASVLGAAPVALVGEAMTRLGQFAGAGSGLPCPLCRDSLRAREEYVVATREGTFLVSTS
                     RTPSAAEKDVRTVHVLKDITDRLEAERRYRELFDSIQEGLFFASPDGRFLDVNGALVR
                     MLGYGSREELLRAEVSRHLYPDPASRKRVLSALNERGVLRNYEESLRRKDGSLLHSLQ
                     YITAVRDAHGEIVQIRGLMLDVTEQRLIQTQLQRERDFNQKILNTTQSMILVLDTTGL
                     ITYANKRCSDAGYHGEELIGCRLVDWVESVQRLTFEAALEATAQGEHVENLELHMRRS
                     DATEGQFSVSLSPMRDEQGVVNSIVVVMTDITDAVILQAKLAHAEKMATIGRLVSGVA
                     HEVNNPLAAILGFTDLLLENPEVPGKAKEDLQIILQETQRTKDIVQDLLSFARQRPAR
                     RERVQINAVLRQTLKLRSYDFASHGVEVIEMFEEDLDPVLGDAQELQQVFLNIVNNAY
                     DAVQEARRTGRIQIRTSQREKQVEVTVTDNGTGISQPERIFDPFFTTKQPGKGTGLGL
                     SICYGIVRAHAGEIQCWNNAGGEGSTFAVRIPAATESAFSAAATEAGR"
     gene            2482..2880
                     /locus_tag="VGR03_10395"
     CDS             2482..2880
                     /locus_tag="VGR03_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV1994730.1"
                     /translation="MKAAATSRRTAPVLLIEDEPSVSAFLRAALERRGYSVVPVSSGA
                     EGLDRLSAGDYLGIISDIRMPGRVNGAEVHDWVQQNRPELSTRLILITGDTANRETQA
                     LLDNSGTPWIEKPFRVRQFLSIVEKTFGAP"
     gene            2877..4316
                     /locus_tag="VGR03_10400"
     CDS             2877..4316
                     /locus_tag="VGR03_10400"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV1994731.1"
                     /translation="MSDAIKTKLLIVDDEQSIRRLCMTIGRTLGFACSEAENAETALA
                     SLDTEAPDLVLTDLRLPSLSGVELLRQTKAILPRTEVAIMTGHGSIESAVDAMKLGAY
                     DYIEKPFRVEKLRLLLQRMAEKVCLVTENQFLRERVSTEKNLDGIIGASAKIQDVLRM
                     ISRLKDTRTPVLIAGESGTGKELVARAIHFRGAMAKTPFVAVDCGSLVPTLMESELFG
                     YEKGAFTGATKSKSGLFQAADGGTIFLDEIGELPLEMQAKLLRVLQEKEVRPVGSNDR
                     VNVDVRVVAATNRDLEAAYRAGTFRKDLYFRLNVVTVHLPALRERRSDIPILVHHFLD
                     HLATGENVQVTSAAMKSLLQYDWPGNVRELENCISRAITLGDRHVIDAHDLPPSIRTD
                     QGSAAGHSGGQPDAPSFSTPALAEMERVTILRVFEQARGDKALAGKMLGISRATLYRK
                     LKRYNIAMKPETVSSEANTRTATRSAGST"
     gene            4606..5289
                     /locus_tag="VGR03_10405"
     CDS             4606..5289
                     /locus_tag="VGR03_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994732.1"
                     /translation="MRQANVVILTDENEFADLLAGCWQVERNVPAITVLNSELWEKTP
                     QPGSDLLVIGPLRDGKLPGILRSLDPASAVILCGSGDLHELTELRARFPRLLYIPLRE
                     DWAHTLLLVAGEALRRTEALHLARLYHTAALQSAHQATLGRYILEMRNSVNNALTSVL
                     GNADLLLIEPGQLSAQSLKQLRTIQSMALRIIEIMQRLSSLASENSMAENESQREIDE
                     APANTSGRR"
     gene            5416..5865
                     /locus_tag="VGR03_10410"
     CDS             5416..5865
                     /locus_tag="VGR03_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994733.1"
                     /translation="MPRNEQEEEFKNPGRPAISRRAFARRAAIASAAATLAPGALLPR
                     AIESDSFDSTLTTIQSPQDNSKLSAEGRTEVEARVHTVLTRYGKRLSGEQKVDIRRLQ
                     TLLQPQLESLRAYSVSNGDSPALYLKPLVEREKRPARTNPAVAAQKH"
     gene            5878..7287
                     /locus_tag="VGR03_10415"
     CDS             5878..7287
                     /locus_tag="VGR03_10415"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV1994734.1"
                     /translation="MVGEEILYLPLSDLAKRIQAKKLSPVELTESYLARCEKLNSRFN
                     AYAKLVPDFALEQARKAEKEIHGGHYRGPLHGVPYAAKDLLSVNGFPTTWGAKPYANQ
                     SFRSDATVIEHLNRAGAVLLGKAAMIELAGGMGYRFASASLTGPAKNPWNPEYWTCGS
                     SSGSGAIMAAGLAAFAIGTETWGSIICPSGFCGVSGLRPTYGRVSRFGAMALSYSMDK
                     IGPICRSAEDCARVFAVIAGHDPKDRSTLPLDLAAFTYSPATEPRKGALRLGRVVNAW
                     RTLEPGVAKCLAEAERVLRKRFPGLKDAELPEGPFEAAGNVIISVEGASAFGDLIRSG
                     RVADLTDPLGQIAGYVNETISASDYVTALRVRENLQKKMADLFDSFDVLVTASMPVAA
                     CKLDTNLETGLDFPDPLGGIGNLCGLPALAVPCGFNEIGLPVGLQFITRAGNDLAVIQ
                     VARAYQLLTGWHLKHPKIN"
     gene            complement(7280..9703)
                     /locus_tag="VGR03_10420"
     CDS             complement(7280..9703)
                     /locus_tag="VGR03_10420"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV1994735.1"
                     /translation="MSQRIQLEAGWRLGPTELHALLECTQDFVLVLGKSGTIQYVNAA
                     VQRVLGYSPDDLLGENVERLFHPHDTENLRRELRGFTAKPDTAIARRCRLRSKDGSWR
                     KFEAAGRSYLCETGEVAVACSYRDVTELSRIEAERRVISEVIHALNQTSNLDELLERI
                     HQALKKVLYAENCFIALHGPEAGTFHFPFFVDQFDTVPPPQKVGRSCTAYVFRTGRAM
                     LIAQKEFDVLVERGDVELVGSPSPAWLGVPLKTPTATIGVLVVQHYENENVYDKRDLE
                     FLDSVGGTIALAIERRRSEDALRKSESMFRLLFAHNPLPTWVFDKETLRFIEVNEAAV
                     TQYGYSGEEFRNMTFRDIRPEEEIKQFDDFWNENFDARGHSGHWKHRKKNGQLIEVEI
                     VAHELEYGGRPVRLVVARDITERDWLEQQLRQAQKMEAVGRLAGGVAHDFNNLLMVIK
                     GHTELLLGSLPASQQTHRKIEQIDRAADRATTLTRQLLAFSRMQVLQPRVLDLGGIVE
                     EMGVLLPRLIGEDIELSIRTQRDLGKIRADASQMEQMIMNLAVNARDAMPAGGRLTIE
                     TANAELDASYGAKHPIVNSGLYVLLAVTDTGAGMDETTKAHIFEPFFTTKEKGKGTGL
                     GLATVYGVVKQSGGFIWVYSEVGRGTTFKIYLPRVDQPVETCGAAPTVGEIPRGTETI
                     LLSEDEQDVREVTREFLESGGYTVIEARDGTEALRLAERHAGEIDLLITDMVMPGMTG
                     QELASRLRSLRSIGRVIYISGYTEPGAGRISGNDGFSRLLTKPFSRLVLLRTVRDLLS
                     VQEPAADSN"
     gene            10072..>10289
                     /locus_tag="VGR03_10425"
     CDS             10072..>10289
                     /locus_tag="VGR03_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994736.1"
                     /translation="MHEILSHMFVLGLPIAEKILRSVVVYAFLVVGLRLAGKRELAQL
                     NPFDLVVLLTLSNTVQNAIIGDDNSVTGG"
BASE COUNT         2067 a         3126 c         3075 g         2021 t
ORIGIN      
        1 gtggtagatt cggcacccca cgtctcatct tgaattgact caatttgaga ttgcccggta
       61 gcgtagacgt gctcgctcac caggaattag atgaacgcgc gcagagcccc cgtcccccat
      121 gcgtggcgcc aagacgatcc cgcttcgcac cgccccttct gtcaagggtg ccggcgataa
      181 tctgcacggt gcgtcttctt gcgctgaaga gttgatggat ctggcgctcg acgcggcgca
      241 atccaagaat ctgcctgact ttctccggca attctccgca cgagccggcc ggatgctcaa
      301 agcgaattgg tgcggcgttg cggtcttcca aggggaagaa gcggacctct attcgagcaa
      361 ctcggaaccg ggcaaaaata cgcaaacgac acggtctctc gcaaaggcca tccttgagac
      421 cgcttatgag acggagaatg caatccacgt tgtcaactct gctctaaagg agttccctgc
      481 ggcagaccgc gctgacacca gcctcgagac cgtcttgacg cgaattgatt cgggcgacgg
      541 cgaaagactt ggatgccttt gcctgctgag gaaaccgcag ccgctgggag atccggacct
      601 ccggcttctg cgcgcgctgg cgagccacgc cgcgctatcc ctcgtgaatt ttcgacggtt
      661 ctcgcagctc gagcgctcga agcgtcaatg ggtggaagac atcgacgcca tcaccgacta
      721 tatcgtcgtc cacgatcgtt cgtggcggat tcttcgcacg aaccgtactc tggcgtcggt
      781 gctgggcgca gcacccgtcg cgctggtcgg agaggcgatg acgcgtctcg ggcaattcgc
      841 cggcgcgggc agcggccttc cttgcccgct gtgccgcgat tctcttcggg cgcgggaaga
      901 gtacgttgtg gcgacgaggg aaggcacctt tctggtttcc acgtcgcgga cgcccagtgc
      961 cgccgagaag gatgtgcgca cggttcacgt cctcaaggac atcaccgacc gcctcgaggc
     1021 cgagaggcgt taccgcgagc tgtttgacag catccaggaa ggattgttct tcgcgtctcc
     1081 cgacgggcgc tttctcgacg tcaacggcgc tctggtgcgg atgctcggct atggctcgcg
     1141 ggaggaattg ctgcgcgccg aagtcagccg ccacctgtac cccgacccgg cttcgcggaa
     1201 acgggtccta agcgctctga acgagcgggg cgttttgcgc aactacgaag aatcgctgcg
     1261 gcgcaaggac ggctccctgc tccattcact gcagtacatc accgcggtgc gtgacgcgca
     1321 cggagagatt gtgcagattc gcgggctcat gctcgacgtc accgaacaaa ggcttatcca
     1381 gacgcagctg cagcgcgaac gcgattttaa tcagaagatc ctgaacacca cgcagagcat
     1441 gattctcgta ctcgatacca ccggcctgat cacctacgcc aacaagcgct gctccgatgc
     1501 cggttaccac ggggaagaat tgatcgggtg ccggctcgtg gattgggtgg agtccgtgca
     1561 gcgccttaca ttcgaagccg ccctggaggc gacggcccaa ggtgagcacg tggagaatct
     1621 cgagctgcac atgcgtcgaa gcgatgccac cgaaggccag ttttcggtca gcctcagtcc
     1681 gatgcgtgat gaacagggcg tggtgaacag cattgtcgtc gtgatgaccg acatcacgga
     1741 tgcggtgatc ttgcaagcca agctggcgca cgcggaaaaa atggccacca tcgggcgcct
     1801 ggtctccggt gtggcccatg aggtgaacaa tccacttgca gccatcctgg ggttcaccga
     1861 cctgcttctt gagaatccgg aagtccccgg caaggccaag gaagacctgc agatcatcct
     1921 gcaagagacg cagcggacga aagacattgt gcaggacctg ctcagctttg cgcgacagcg
     1981 tccggcgagg cgggaacggg tccagattaa cgccgtgctg cgccaaacgc tgaagctccg
     2041 gagctatgat tttgccagcc acggggtgga ggtgatcgag atgtttgagg aggatctcga
     2101 cccggttctg ggcgacgcgc aggagctgca acaggttttt ttgaacatcg tcaataatgc
     2161 ctatgacgcc gtgcaagagg cccgccgcac gggcaggatt cagatacgga ccagccagcg
     2221 cgagaagcaa gtcgaggtga ccgtcacgga caatggaaca ggaatctccc agccggagcg
     2281 gatcttcgac cccttcttca ccacgaagca acccggcaag ggcaccggct tggggctgag
     2341 catctgttac gggatcgtgc gcgcgcacgc tggcgagatt cagtgctgga acaacgccgg
     2401 tggggaaggc agcacattcg ccgtgcgaat ccccgcggca accgagtccg ccttttcagc
     2461 cgccgcgacg gaggccggac gatgaaggca gccgccacaa gccgcagaac tgcccccgtc
     2521 cttctgattg aggatgaacc gtcggtgagc gcgtttctcc gcgccgcgct ggaacggcgc
     2581 ggctactcgg tcgttcccgt ctcgtccggc gcggaaggac ttgaccggtt gtctgccggg
     2641 gactacctgg gaatcatttc ggatatccgc atgcccggcc gagtcaatgg agcggaagtt
     2701 catgactggg tccaacagaa ccggccggag ctgagcacac gacttattct gatcacggga
     2761 gacacggcca accgtgaaac tcaggccctg ctcgacaata gcggcacgcc gtggatcgag
     2821 aaaccctttc gggtcaggca attcctttcc atcgtggaaa agacgttcgg agcaccatga
     2881 gcgacgccat caagactaaa ttgctgatcg tggatgatga acaaagcatc cggcggctgt
     2941 gcatgaccat cggccgcacg ctcggctttg cctgctcgga ggcggagaac gcggagacag
     3001 ctctcgcgag cctcgacacg gaggcgccgg acctagtcct gacagatcta agacttccca
     3061 gcctgtccgg ggtggaactc ctccggcaga ccaaggccat cctcccgcga accgaggtgg
     3121 ccatcatgac cgggcacggc tcgatcgaat ccgcagtgga cgccatgaag ctcggcgctt
     3181 acgactacat tgagaagccg ttccgggtgg agaagctaag gctccttttg cagcgcatgg
     3241 cggagaaggt atgtctggtg acggaaaacc agtttttgcg ggagcgggtg agtacggaga
     3301 agaacctcga cggaatcata ggcgcctcgg caaaaatcca ggacgttttg aggatgattt
     3361 cgcggctgaa agatactcgc accccggtct tgatcgcggg ggaaagcggg accggaaaag
     3421 agctggtggc ccgcgcgatt cacttccgcg gagcgatggc caagacgccg tttgtcgccg
     3481 tggattgcgg atcgctcgtc cccacgctga tggaaagcga acttttcggt tacgagaagg
     3541 gcgccttcac gggcgcgaca aagtcgaaat cgggactctt ccaggcagcg gacggcggca
     3601 caatctttct ggatgaaatc ggtgagctgc cgctggaaat gcaagcaaag ttgcttcgcg
     3661 tactgcagga aaaagaagtc cggccggtgg gcagcaacga ccgtgtgaac gtggacgtgc
     3721 gcgtcgtcgc cgcaaccaac cgtgacctgg aggctgccta ccgggcgggg actttccgga
     3781 aggacctcta tttccgccta aatgtcgtta cggtccacct cccagctctc cgcgagaggc
     3841 gttccgatat ccccatactc gtgcaccatt ttctcgacca cctcgcgacc ggagagaatg
     3901 ttcaggtgac ttcggcggcc atgaaaagcc ttctgcaata cgactggccg ggcaatgtgc
     3961 gcgagctgga aaactgcatc tcccgggcga taaccctcgg ggatcggcat gtgattgacg
     4021 ctcacgatct gccgcccagc atccgaacgg atcagggttc cgcggcaggc cattccggcg
     4081 ggcagcctga cgctccttct ttttccaccc cggcgctggc ggagatggaa cgcgtgacca
     4141 ttctgcgtgt cttcgaacag gcgcggggag acaaggcgct ggcggggaag atgctgggta
     4201 tcagccgcgc gacgctctac cgcaaactca aacggtataa catcgcaatg aagcccgaga
     4261 cggtctcctc cgaggcgaac acgcggaccg ccacgcgttc agcggggtcc acatagaccg
     4321 ccaaagcacc gtcccgtgag acaagattgc ttgagacgcc tcgtttcagg ctgagacggt
     4381 tgagtcactt tcatccctga ctcgattcac aaccccttat ttatctatca cttacctccc
     4441 tgaaccggac tccactttgt ggtgcgatgc gtgcactctt cctgctgagg gcactgcgtc
     4501 tcgacccgct ctgggtgtat cgagacggcg caaacatgag gcgggaaagc tctagacggg
     4561 cgcggttaat cgaaggcgat tcaagcttca cgggagccaa agtccttgcg gcaagcaaac
     4621 gtcgtcatac tcacggatga aaacgaattt gcagacttgc tggcagggtg ctggcaagtg
     4681 gagcggaacg tccctgccat caccgtcctc aacagcgagc tctgggagaa gacccctcaa
     4741 ccgggcagcg acctcttggt gatcgggccg ctgcgcgatg ggaagctgcc gggaatcctg
     4801 cgatctctgg atcccgcatc ggcggtgatt ctctgcggga gcggggactt gcacgagctc
     4861 actgagctcc gcgcgcgctt tccgaggctc ctgtacattc cgctacgaga agactgggcg
     4921 cacacgctgc tgctggtcgc cggggaggcg ctgcgccgca ccgaggcgtt gcatcttgca
     4981 cggctgtatc acacagcggc tctgcagagc gcgcatcagg ctacgctcgg ccgttatatc
     5041 ttggagatgc gcaatagcgt caataacgcc ctgacgagcg tcctggggaa tgccgatttg
     5101 ctgttgatcg agccggggca gctttccgcc cagtctctga aacagctcag gacgattcag
     5161 agcatggcgc ttcggatcat tgaaatcatg cagcgactct catcgctcgc gagcgagaac
     5221 tcaatggctg agaatgaatc tcaacgcgag atagatgaag cgcctgctaa cacgtcaggg
     5281 cggcgctgac ccgccgccgc aatccgcccc cgatcgcgtc gcttcgcact cggccacttt
     5341 ctcccgttga ccgctcctcg cggattcagt agcctctcgt tgcagcattt ccatcgtgtc
     5401 acgaaggagt agtccatgcc gcggaatgaa caagaggaag agttcaagaa tccgggacgg
     5461 cccgctattt cccgccgtgc attcgctcgc cgcgcggcca tcgcttccgc ggcggcgacg
     5521 ctggccccgg gcgcgctcct ccccagagcc attgaatcgg attccttcga tagcaccctc
     5581 acaacgatcc agtcacccca agacaactca aaactctctg cggaaggccg gacggaggtc
     5641 gaggcccgcg tacacactgt tctgacacgc tatgggaagc gtctctccgg tgagcaaaag
     5701 gtcgacatcc ggcggctgca gactctgctt cagcctcagc ttgagtcgct tcgggcctac
     5761 tccgtttcca acggagattc tccagcgctg tacctgaaac ccctcgtcga acgcgagaaa
     5821 cgcccggcgc gcacaaaccc ggctgtggcc gctcaaaaac actgaaagga cgcgggtatg
     5881 gtcggcgaag aaatccttta tctacctctc agtgatctgg cgaaacgcat ccaggcgaaa
     5941 aaactttccc cggttgagtt gacggaaagc tatctcgccc gatgcgagaa gctcaattcc
     6001 cgtttcaatg cctacgcgaa gctggtaccg gatttcgcct tggagcaagc gcgcaaagcg
     6061 gaaaaggaga ttcacggagg gcactaccgc ggccccctcc acggcgtgcc gtatgctgcg
     6121 aaggatctcc tcagcgtaaa tggtttcccg acgacgtggg gggcgaagcc atatgccaat
     6181 caatctttcc ggagcgatgc gaccgtcatc gagcacttga accgggccgg tgcggttctg
     6241 ctcggcaagg ccgcgatgat cgagctcgcg ggtggaatgg ggtatcgatt cgcatccgct
     6301 tccttgaccg ggccggcgaa gaatccctgg aatccggagt attggacgtg cggttcgtct
     6361 tcggggtcgg gcgccatcat ggcagcgggc ttggctgcct tcgcgatcgg aaccgaaacc
     6421 tggggatcca tcatctgccc ctccggattt tgcggcgtca gcggactgcg gccgacctac
     6481 ggccgcgtga gccggttcgg cgccatggcc ctcagctact cgatggacaa gatcggtccc
     6541 atctgccggt ccgccgagga ttgcgcccgc gtattcgcgg tgatcgcggg gcatgatcca
     6601 aaggaccgca gcactcttcc gctggatctc gctgcattca cgtattcacc ggcaaccgag
     6661 ccgcgcaaag gagcattgcg cctcggacgg gttgtgaatg cgtggcgaac gctcgagccc
     6721 ggcgtcgcca aatgccttgc tgaagcggag cgcgtgctcc gcaagcgctt tcctggcctg
     6781 aaagatgctg aactcccgga agggccgttc gaagcggcgg gcaacgtgat tatctccgtc
     6841 gagggggcat ccgcattcgg cgacctcatc cgctccggcc gggtggctga cctgaccgac
     6901 ccgctcgggc agattgccgg atacgtgaac gagacgatct cggcgagcga ttacgttaca
     6961 gcacttcgtg tccgcgagaa cctacagaag aaaatggcgg atctcttcga ctctttcgat
     7021 gttcttgtga ctgcgtccat gcccgtcgcc gcctgcaaac tcgacacgaa ccttgagacc
     7081 ggcctggact tccctgaccc gctgggcggc atcgggaatc tctgcgggtt gccggcgctc
     7141 gccgtccctt gcggcttcaa cgaaataggt cttcccgtgg ggctgcaatt catcacccgt
     7201 gccggaaatg acttggcggt gatccaggtc gcgcgtgctt atcagctgct taccggctgg
     7261 cacctcaagc atccaaagat taattagaat cggcggcggg ctcttgcacg gagagcaaat
     7321 cgcgcacggt gcgcaacagg acgaggcggc tgaagggttt ggtcaggagt ctcgagaagc
     7381 cgtcatttcc ggagatccgg cctgctccgg gctccgtgta acccgatatg taaatgacgc
     7441 gcccgatgga ccggaggctc cgcaggcgtg acgccagctc ctgcccggtc atccccggca
     7501 tgaccatgtc agtgatgaga agatcgatct ctccagcgtg tcgctccgcc aggcgaaggg
     7561 cttccgttcc gtcccgagcc tcgattactg tatagccgcc ggactcgagg aactcgcggg
     7621 tgacttcgcg cacgtcctgc tcgtcctcgc tcagcagaat ggtttccgtg cctcgaggaa
     7681 tctccccaac ggttggggcg gctccgcagg tctcgaccgg ctggtccacg cggggcagat
     7741 aaatcttgaa ggtcgtccct ctgccaacct cgctgtaaac ccaaataaac ccgccacttt
     7801 gtttcaccac accataaacg gtcgccagcc ccagtccggt tcccttcccc ttttccttcg
     7861 tcgtgaagaa gggttcaaaa atgtgggcct tcgttgtttc gtccattccg gctccggtat
     7921 ctgtgaccgc cagcagaaca tagagcccgg agttgacgat ggggtgcttc gcaccgtagc
     7981 tcgcatccag ttcggcgttg gccgtctcga tggtcagccg cccgccggcg ggcattgcat
     8041 cgcgcgcgtt gaccgccaaa ttcataatca tctgttccat ctggctcgcg tccgcccgga
     8101 tcttgccgag atcgcgctgc gtccgaatgc tcagttcgat gtcttcaccg atcaggcggg
     8161 gtaagagcac gcccatctct tcaacgatac cgcccagatc cagaacgcgc ggctgaagaa
     8221 cctgcatccg gctgaaggcc agaagttggc gagtgagtgt tgtggcccgg tccgcggcgc
     8281 ggtcgatttg ctcaatcttc cggtgcgtct gctgcgatgc cgggagggag ccgaggagca
     8341 gctctgtgtg gcccttgatg accatcagga gattgttgaa atcgtgcgcg acacctcccg
     8401 ccagccgccc gacggcctcc atcttttgcg cctgccggag ctgctgttcg agccagtccc
     8461 tctcggtgat gtcccgcgcg acgacgagcc ggacgggacg tcccccatat tccagttcgt
     8521 gcgcgacaat ctcgacctcg atgagttgcc cgttcttctt gcgatgtttc caatgtcccg
     8581 aatggcctct ggcgtcgaag ttttcgttcc agaagtcgtc gaactgcttg atttcctcct
     8641 cgggccggat atcccggaac gtcatgtttc ggaattcctc gccggaatag ccgtattgtg
     8701 ttaccgcggc ttcgttcacc tcgatgaagc gcagggtctc tttgtcgaag acccaggtgg
     8761 gaagggggtt gtgagcgaac aagagacgga acatggattc actcttgcgc aacgcgtctt
     8821 ccgagcggcg ccgctcgatc gccagtgcga tcgtcccccc gacggagtcc aggaactcga
     8881 ggtcgcgctt gtcgtagaca ttttcattct cgtaatgctg cacgacgagc acgccaatag
     8941 tggccgtcgg cgttttcagt ggaacgccaa gccatgcggg tgaaggagaa cccaccagct
     9001 ccacgtcgcc gcgctcgacg agaacatcga attctttctg cgcaatgagc atcgcgcgcc
     9061 ccgtgcgaaa gacgtaggcg gtacagctcc ggccgacctt ttgcggagga ggcacagtat
     9121 cgaattggtc cacgaaaaag gggaaatgaa acgtcccggc ctcgggtccg tgcaaggcga
     9181 tgaagcaatt ctcggcgtaa agtaccttct ttagagcctg gtggatcctc tcgaggagct
     9241 catcgagatt gctggtctga ttgagcgcgt ggatgacttc cgaaatgacg cggcgttcgg
     9301 cctctatgcg gctgagttca gtcacatcac ggtaactaca ggcaacagcc acttcaccgg
     9361 tttcgcatag ataactgcgt ccggccgcct cgaacttgcg ccacgatccg tccttggacc
     9421 ggaggcgaca tcgtctcgct atcgccgtat ccggcttagc cgtaaagccc cgcaattctc
     9481 gccgcaagtt ctccgtatca tgaggatgaa agagcctctc cacgttttcg ccaaggagat
     9541 catcaggaga gtagccgagc acccgttgga cggcggcgtt cacatactga atcgtcccgc
     9601 tcttccccag cacgagaaca aaatcctgtg tacattccag gagtgcatgc aactcggtcg
     9661 gtccgagccg ccatccggct tccagttgaa tgcgttgcga catggtagcg gaaaggtcct
     9721 gcctcgggca tccccagggc gggaaattct cccggagtct ccttagagcc tcacttgctc
     9781 gaacggcaag acaccacgtc gaacgcagaa cagtgttctg cccggtcgaa ttgccagtgg
     9841 gctcttatct atctattttg cgggagtttt tccaaacttt gagcagcggc gatgggcctt
     9901 gtctccaaac cgtaatgaac cgaaagtact agcgagaaaa aagggagaaa ccgcggtttt
     9961 cttccgcatc aaggtttctc atgctaggct ttcgtcgccc gccgcggaga gtgcattcca
    10021 agcgctatgg atccttgacc ggacggcgcg tcaagaggag gaacagagtt catgcacgaa
    10081 atcctgtctc atatgtttgt actgggccta ccgattgccg aaaaaatcct gcgctccgtc
    10141 gtcgtctacg cctttctggt cgtgggcctg cgcctcgccg gaaaacgcga gttggctcaa
    10201 ctgaacccgt tcgatctggt ggtcctcctc acgctgtcga atacggtgca aaacgccatc
    10261 atcggcgacg acaactcggt caccggggg
//
LOCUS       DASYVO010000308        25735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_93563, whole genome shotgun sequence.
ACCESSION   DASYVO010000308 DASYVO010000000
VERSION     DASYVO010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 25735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25735
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93563"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VGR03_10430"
     CDS             complement(<1..344)
                     /locus_tag="VGR03_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994737.1"
                     /translation="MDLEKIRGAAERVARSEGLEIVDVEWKIGKQRFLRVYIDKVPGG
                     REAVEVPGGREAANTPKPTAAMSDAAGEMGTTDVAHDPYPKINHSDCQRVSQQLSVIL
                     DVEELIPGPGYVL"
     gene            complement(728..1456)
                     /locus_tag="VGR03_10435"
     CDS             complement(728..1456)
                     /locus_tag="VGR03_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994738.1"
                     /translation="MSNPGEDFYDLTSPPYPQQGDIFPNVPLISPPPGPHLVILRETD
                     GKPWTPKPGLLQASAEQLVNAFDDVPEYIAVSAERGLAAILTQTCDLDQGQWLVCPLI
                     TIEGTNIDEGNLLAGKYASLLGMPRHPRGYFETGCLFLPQCFSIRRESVQQKDRVASL
                     TLSAQHALSDKLSETLTRVWGFSPGEIVLQTGKYRCVRCFQFYDVKNEVVEFQAGQKF
                     GDCPDCLKIKKRAQWRPLRKHQKY"
     gene            complement(1735..2088)
                     /locus_tag="VGR03_10440"
     CDS             complement(1735..2088)
                     /locus_tag="VGR03_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994739.1"
                     /translation="MSAKKPKVLNFKVVGEEPVNAVPALYSNFLAVSQVGNDVQFEFV
                     FLDLNKVAMMMQEIKSEEKPEILELQGKTVAKVVMPAANVLQLKGHFETIFRAIEEIL
                     QKQPEAKHGRTSNAG"
     gene            complement(3197..3559)
                     /locus_tag="VGR03_10445"
     CDS             complement(3197..3559)
                     /locus_tag="VGR03_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994740.1"
                     /translation="MFKRLMILGIAGLVFVLGANYLLVYTLNQQITRERERQDRTYWS
                     TFNAIEQFGEHADKVTEQKAKAALDEARVKGLSKIRARVLQNYLEDLERCYQGERESC
                     KKANTDMNDAIRVPAERK"
     gene            complement(3635..4498)
                     /locus_tag="VGR03_10450"
     CDS             complement(3635..4498)
                     /locus_tag="VGR03_10450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEV1994741.1"
                     /translation="MESTLGLRAGQPNSSPLEGSEPFRTQQAESGLRQLARREWWLWF
                     SAFFVATLSALAFVLSSFPSFFRHSEHFYEIRSDQARWGILCLLLLFNGWMLYRQWSF
                     RRQRKQLSGQNAGADAQSGQISDSSGIDPVTGLHTRAFIEQQLGKEIARARRQNTALS
                     LATVHLDEFAEINNRYGPSAIDAALKELARRLKKACRGSDFAARLANDDFLLVLPECN
                     LGEVKSVLNRLGALEMVCSGRKMNLVYTTGWVDYQPGDLPADLLKRAAQLLHLYENAA
                     KDSLSSTLAPH"
     gene            complement(4721..5308)
                     /locus_tag="VGR03_10455"
     CDS             complement(4721..5308)
                     /locus_tag="VGR03_10455"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEV1994742.1"
                     /translation="MSTAAAWAQAPAAAGSAASGKVGVISIRLAIGSTAEGKLAQAEL
                     QSQFAPRQNEIENLNKQINDLQQRLQAGANKLSQEEEARLRQQGQRLAQRLDRMNTEY
                     QEDVNAALGDITDRIGRKMVDVLDRYARENGYSVVLDSSAQSTPIIYASTQIDVTQDI
                     VRLFDQAYPVKAGAATQPKPAAPKPAAAQPPAAKP"
     gene            complement(5635..9993)
                     /locus_tag="VGR03_10460"
     CDS             complement(5635..9993)
                     /locus_tag="VGR03_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994743.1"
                     /translation="MKWHKSARNILLAIAFASLAVSGCGSKSAANQVLVSVLGSFSVM
                     VPTQAQTIIANVTGATDVSATFDCSYTTTQNPTTAIPSPKPSASAACDTAKTASGDPA
                     VGTLTPIASTSTTVASTATFTAPKVFPDQTKLPNVIVTITATANADKKKTGKFSLAFD
                     SGIRITLSPATATLGTVETQLFLAKDFSGTIIKPDQLTWGVTFESTARTNSALCNGGS
                     NDCGSVDKTTGKYTAPAAVPVAAPASTTTPVNAAGIVTLFVFSNVDNARIAQAAITIV
                     KAGDITFSGISPSVVPQGALQQDIFLAATNATSQLGVTLTAPCGDIPVDQQSQVKVIF
                     APGTTSSFIGARIRLNKTQLTGAGHYQLQVTGSNPSVKVTGGPFPLDIIPVRPTIVSS
                     VPENLQESTLAQDGITIDGGYFGTNIGTLPSCTVSPTITPQFNNTSSSTLLQPNSVTS
                     RRLTGFLPTPSATNPNAGLFPLSVKYSTSPGPFAVPTPATAYTNIAVIPDYAGNNPPG
                     APSQLAIPGNAVPASPTMPSAIALDSVKGYAVVTLAGQNAFLTAAPTVNTANNIQFIN
                     LASGTPTLTALVSSQGFLATGVAVDDQLHVAAVVNYASRSLTVHSIPSGMLLGTVDLS
                     GVIPPPATSGSSFVAPFPYSVGIDPFSHHALVAFASTNVGLIINLDTSSSVLPNNGCI
                     LPGQTATPNFCPIAYVTLNSGANPQIAFEAGAHLAYATPGGAGQLSAVNLANPSVGSV
                     GVASATRDTSNVVTITTSASHNLNPGNPGTVLISGLPLGSTNKTNFNGSFSVGNVLDA
                     THFQYSQADKADTATCTTGCLASSGVPFLTYAVSPSTLGIAINPITRRAILVDPNATS
                     SQINFIDPISQAVSFMTVFVGATGTMTGTPELGAATVAFQPFTNTALSFNSNLNQISL
                     LDPSLLQRSKIISTGPGQVAKATASFTPAGPSSTAITVNLPGALAADSTNNLALAVNS
                     GSDNISFFTLGTIKALHIEHVIAPLIDDPNDKNIPVKANLTAAIKITQGATSVATGPV
                     KIFGSGFSGSSKVRLDTIDVFTLGATVVLKGSQELDVTFPPNFFTGPHHFALDVFNSA
                     AVTSNVVDFTVVEEILLDPCNVTPTSAGTPAAPGGVAIDEVHNLAVVTNTGAGCNQVS
                     VFSLNPANILNKTVKTIPTGDTPTGVAVLPRLAYTGQAAGTSGVAVVTNSGSNSVSLI
                     DLVNRVQVLDASAKPITVNVGTGPSGVAIDQETNLAVIANTGSNTVSTIDLTPLTATP
                     TIGTLAPTSVAVDQNPIAVAIDPDRGTNGRGLAVVTCLVLNGASSPFGALDAVDIGLA
                     TPVRSTSGSTSFLSSTPTGLVFDPSVSPALFYAVSTQGNVITAFNPDTGGTTTIKVGI
                     NPNAIAYNFQTGTILTVNSLSNTISIVDSQTFATKATLGIGGTSKFAAAIQTFTNLAV
                     IADQANNRVILFPLPK"
     gene            complement(10095..13055)
                     /gene="bamA"
                     /locus_tag="VGR03_10465"
     CDS             complement(10095..13055)
                     /gene="bamA"
                     /locus_tag="VGR03_10465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEV1994744.1"
                     /translation="MIAMVFHFAIASPAAPRENPHADASSLAHSYLFTVYFDYATGKD
                     VIDDRSAAHPQAAARQQQFVIERIDFIGNRRVRSDTLKARIFSRDGDVYNEETLRRDF
                     QALWNTQFFEDVKLRVEDSAKRTDAKVIIFEVRERPQIRRIRYDGIHSVSESDILDRF
                     KERKVGLSVESQFDPTKIKKAEVVLKDLLGEHGRQFAKVTPQYERIASSNAVILVFKI
                     EEGPKVKVGKIKFTGNHAYSDRKLIRAMRHDRPYSIPLYFWYIPVLTKTYDRQKLSED
                     LEVGVRGLYRDNGYFKVSVGEPLLENIDTESYRLGVPLIAGRSHGKAVNITIPIEEGE
                     RFRMGTLKIVSSDPDKALSLKVEALKGAFPLKQGDLFSTEKVRKALETYGKIYGEYGF
                     IDFTPEPDTEVDDVNKIINLTLRFDEQKQYFVRRIDFSGNTTTRDKVIRRELAIDEGQ
                     LFNKRLWEMSILRLNQLDYFDKIEADKAAEIKRNSKEGTVDINLKLKEKGKQSIGLQG
                     GVSGLAGSFIGLTYQTNNFLGLGETLTFSAQFGDLSRSFMFGFTEPYLFDRPISSGFT
                     IFSSRYKFDQARQAALYTGQSVSINPQFVQNYNQDSTGVTVFASYPVRKLSFTRIGLQ
                     YGLTRTNITAFNDASKLLFTQLQFRSVAGPSALNGIVSSAITPTITYNTVNNPINPTG
                     GKSYFYSLGFAGLGGNVKTITNVFDWKYYHPVNKRRNVLGFHASGAFITGYAGGEPPP
                     YSRFYMGGESDIRGFDIRSITPVTFIPVASTQQFSYLCGTCLNGFGQPTQRTVNVPVL
                     GYTISFPGGDLQSYGNVEYRIPIVGNIVQVGLFFDGGTNGILRKNALKLDPTGFSNLQ
                     TQFPDARKLAGLTQQLSIAPGTNFRLRGSTGIEFVVQLPIIQAPFRIYYAYNVNRLHS
                     QIVAPPDFLNPSEISDTLGSDGLPIGLLPRTLPPDVWLNQLKPTILQLQNNPGRLNYF
                     EPARTFRFTVSRTF"
     gene            complement(13236..15662)
                     /locus_tag="VGR03_10470"
     CDS             complement(13236..15662)
                     /locus_tag="VGR03_10470"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1994745.1"
                     /translation="MFERYTEKARRVIFFARYEASQYGSPYIETEHLLLGLMREDKAL
                     ANRFLRQQGSIESIRKEIEARITIRERISTSVEVPLSAECKRILNMAAEEAERLGHKH
                     VGTEHLLLGILREEKCFGAEILMERGLRLSTLREELARTSGEKTAAARPKETSLLAEF
                     SRDLTLAASEGSLDPLVGRENEVERVIQILCRRTKNNPVLIGEPGVGKTAIVEGLAQR
                     ISDGDVPPFLADKRILSLDLSLIVAGTKYRGQFEERLKTIMKELMESQNSIVFIDELH
                     TLVGAGSAEGSLDAANILKPALSRGEIQCIGATTPGEYRKSVEKDRSLERRFQSVKVP
                     APDEEQAVQVIQGIRERYEKFHAVSYTDEALRYSVYLSNRYIPDRFLPDKAIDLIDEA
                     GARVKLRQATVPEEVGEVQKRVKFITHRMETAIANHEFEKARFYSEEERKEKENLRGL
                     RERFKLDDASTGIVTREDIEEVVSRWTGIGVTSIKEDEQQKLLRIEPELHKRVISQDK
                     AITALARAIRRSRAGLKSPLRPVGCFFFLGPTGVGKTEVARRLAEFLFGSEKSLVRFD
                     MSEFMEKHSVSKLIGAPPGYVGYEEGGQLTERVRRTPYSVILLDEIEKAHPDVFNILL
                     QVFEDGQLTDGLGNTVDFRNTIIIMTSNLGARHLQKRTSMGFQSGADEAGRKSMEDMV
                     MNEVKRAFNPEFLNRLDEVIIFNPLADEDLLRIIDLLVGQLNDTLIHRQVQIVVSPEA
                     RQWILEKTCADRNYGARPLRRALQKYVEDPLSEALIQGGIQKPATLQIYVAGEGLSFR
                     PVGEATPVVH"
     gene            complement(15664..16440)
                     /locus_tag="VGR03_10475"
     CDS             complement(15664..16440)
                     /locus_tag="VGR03_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1994746.1"
                     /translation="MIQQTQFTSGKPAEDLRNAAVVLESRGLKKTYGSGEKTISVLAD
                     ANLSLRQGEMVAIVAPSGAGKSTLLHLLAALDTPTSGTVYFATKAIESIDDAALAEFR
                     NRAIGFLWQRHQLLPDFTAAENVAMPLLVRGENFASALGMARKWLAEVGLENRVDHRA
                     GELSGGEQQRVAIARALVTGPSVLLADEPTGDLDEKNAWAVFELLERLHRTHKLTSLI
                     ATHNLALAARCDRILALEHGVLRTREAAASASGAPGGEAS"
     gene            complement(16786..18012)
                     /locus_tag="VGR03_10480"
     CDS             complement(16786..18012)
                     /locus_tag="VGR03_10480"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1994747.1"
                     /translation="MRFEWFVAQRYLRSPYRPAVLRLVTLFSTIGVAAGVATLVIALS
                     MNTGFRETLQDRLLGVTAHVSLTRPGTGGIKNYEEMATKLAGATGARSVTPAVYQTVL
                     LSFAGQARGVVTKGIDPERERKSDEALQRIVAGSLDFSPDGDGIDALLIGKQLAEEWK
                     VSPGDYVTLTSPQGRLTPFGLMPRTRRFRVAGVFDSGFYDYDENWCFITLAAAQGLSG
                     AGDVVNVLEFRLNQPDRAGEIAERARQAAGQGYSATTWMEENRALFRALRLEKLVTAI
                     FIGLITFVAGLNILVVLSMTVTDKARDIAILRSMGARREQIRTIFLWLGISIGTAGTL
                     IGLIIGYTFSFVAGNYHLIPLDPQVYAVPYVPFHPSVFDGVWITAVAMGISIAATILP
                     ARAAVRLLPVEILRYE"
     gene            complement(18031..19587)
                     /gene="lysS"
                     /locus_tag="VGR03_10485"
     CDS             complement(18031..19587)
                     /gene="lysS"
                     /locus_tag="VGR03_10485"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV1994748.1"
                     /translation="MQFEPRDQLEQRQKKLEQIQSLGHDPYPREFRWTDTPAALVEKY
                     SQTSGPELEATKNEVRVAGRIVSFRLMGKAGFAHLQGAGKRIQIYVKKDVVGEPGFQL
                     FHLLDLGDSIGVRGHLFRTKTNELSIWVEEIFFLSKALLPLPEKWHGLTDVELRYRQR
                     YLDLIANEKSRQVFVTRARIIQELRRFFDARGYIEVETPMMHPIAGGAAARPFTTHHN
                     TLDIDLYLRIAPELYLKRLTVGGFDRVYEINRNFRNEGISTQHNPEFTMLEFYEAYSN
                     YRDLMDMNEQLFALLAKNITGSTTVKYGEAELDFSKMQRLSMREAIVKYWPAAAGEAP
                     TSEKLGSPGGAQEATSRYNLWAKCAGAPYAGAKGTLKDGEWTGLLFETMAEDKLVQPT
                     ILYDFPTEISPLSKQKPEDPSLTERFEIYVAGMEVANGFSELNDPAEQERRFLAQMAQ
                     GGDEVPKQLDVDYIRALCHGMPPTAGEGIGIDRLTMLLTDSPSIRDVILFPLLRPEGG
                     GESSSEAHGA"
     gene            19832..20557
                     /locus_tag="VGR03_10490"
     CDS             19832..20557
                     /locus_tag="VGR03_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994749.1"
                     /translation="MKDSVRPPTRGKSVKDDWRAWLPEEKAQVFLKQVQQLESGYAML
                     SVSLDEALELKHLGHSGKSLQAMGITSGLCRLLTQTLAGLLRALSEHSKHFGTIPNTA
                     PLDPANFQGQRGQRSARMSSLLSHVLLSHRLQFLHKVSTLEEMVEDLGKDFRYAADDL
                     AEGAPLNPKRMWDEVDADHYDLNTCLREAIVVFKSFLIALPESQLGAFQNTVRQQTEL
                     REVDVPSRHRLIRHRRMTAIAGE"
     gene            20601..21380
                     /locus_tag="VGR03_10495"
     CDS             20601..21380
                     /locus_tag="VGR03_10495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1994750.1"
                     /translation="MSTPSGVSVEGMRASVRTTQGGIRRIRSGGCAAIGPLARTRPSD
                     PNASTSRQSTKPVTEAEAIRLAQAGDAAAFEFLYHLHGRRVYALCLRMVNNPSDAEDL
                     MQEAFLQLFRKIGTFRGESAFSTWLHRMTVNVVLMRLRKKSLPVASLEEMTDPDDETG
                     GPRKDIGAPDLRLSGAVDRVNLERSVEKLPPGYRTVFVLHDVQGYEHNEIADIMGCSV
                     GNSKSQLHKARTRLRDLLQEEVRQKARQVRHAAKTQTAHDD"
     gene            21511..22185
                     /locus_tag="VGR03_10500"
     CDS             21511..22185
                     /locus_tag="VGR03_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994751.1"
                     /translation="MQCKDVEIVVEQEGLAPLPEAARAHVAACSHCQEFVADLATVVS
                     VANELPGEVEPPARVWVSLRAQLELEGIIKTPVVPASGERSSWWHGFNDLFRSRALAT
                     ASVGLLIVAAGVFQLRQTPDASVGPNGIGDRPPSQMAFAQTAKVLNEQEVDLRNMRLA
                     GTSASAVDDSFRQNLQQVDDFIAVCEQRVKVAPEDDLAREYLSNAYQQKAELLSAMMD
                     REGSIH"
     gene            22187..23095
                     /locus_tag="VGR03_10505"
     CDS             22187..23095
                     /locus_tag="VGR03_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEV1994752.1"
                     /translation="MGIGIMGFAALRSDLKRGILPVLGAAAFLCSGLPAAAAGQHLEK
                     HFTVNARPVVVIHNVANGRIEVKSWKNAEVDVVASQTSDKIGFDIEQAGDRIDVTANI
                     LNAAAQPRELEANLQLTVPEETELQLKTETGLIYVEQVMGDMTLESVAGDVHLKEVSG
                     YIIVRTTEGSLVCTQCAGKLDFKSISGGAQVLQPALTNVSLFTTSGNILFDGDFVRTG
                     LYTMKSGKGLVEVRFSGNDSFDLKAQTAMGTVDNQASAYLKPDSHGVKHLASKFTKGL
                     FGTVGLGLAKVELSSYSGTIRILKRD"
     gene            23181..23711
                     /locus_tag="VGR03_10510"
     CDS             23181..23711
                     /locus_tag="VGR03_10510"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV1994753.1"
                     /translation="MGSGKTTIGTLLARQLAWRFVDLDDRIEASAGLPIPQIFERLGE
                     SAFRQIEADQLRAALGRAAEMKESTVLALGGGTYAQPGCPDFLRAAGIPVLWLDSPIE
                     VLLSRCMTMTGRPLFRDEASFSALYAQRLPSYQLADYRVDSSGEPAQVVAEILRRGIV
                     ASGGNAAHLMVEKPPP"
     gene            23742..24455
                     /locus_tag="VGR03_10515"
     CDS             23742..24455
                     /locus_tag="VGR03_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994754.1"
                     /translation="MKFRTVSIALTLGIVACAALSLAAQKKVAPTSGPAVFAQDKGKL
                     TIKLGGQTVGHEEFEIAPSGGGWVAKGTAEIKPPEGSSSKISGSLTLQPDGAPISYDW
                     SAQAEKTNGAHILFANGVASITLEMQGARPFQQDLSFNSPLIAVLDNNLYHQYAVLAR
                     IYDWSKRGVQTFPVIIPQELTPGSITVQSTGSASADGKTYEGLKVNTSDLEILLLLDA
                     NHRLMRLEVPDAKVSVLRD"
     gene            complement(24553..25266)
                     /locus_tag="VGR03_10520"
     CDS             complement(24553..25266)
                     /locus_tag="VGR03_10520"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1994755.1"
                     /translation="MSSLEMDREVIRCKTCGLVQYRTRTGNCRRCVRALPQRLEFLIP
                     PPEPPEEAAAEPTPEKFVNQETVENIGQRIRQLRESRAMTQSQLQSRSKVSRSYLSRI
                     ESGQMTPSLGTLEKISEALSVGLNRFFIPESDGEALLEDPFIQGLRPFLRQLDWEQWQ
                     SILKRLQAISDHVHAAPITLRPIGNPARLPQPASANGNGHIGAGHSNGLAYSHAHGNP
                     QGYPLRRPGGPIFQRPLAR"
BASE COUNT         5285 a         7385 c         7544 g         5521 t
ORIGIN      
        1 aggacgtacc ccggcccggg tatcagttcc tcgacgtcga ggatcacgct caattgctgg
       61 ctgacgcgct ggcaatcgga gtggttgatc ttcggatagg ggtcatgcgc aacgtctgtc
      121 gtccccatct ctcccgccgc atcgctcatc gccgccgtcg gcttcggcgt gttggccgct
      181 tcgcggccac caggaacctc gaccgcttcg cggccaccag gaaccttgtc gatgtacacc
      241 cgcaggaacc gctgcttgcc gattttccat tcgacatcaa cgatttccag accttccgaa
      301 cgcgccaccc gctcggcagc gccgcggatt ttttccaaat ccacgcagag actccccgac
      361 catagccggt ccgcagtaca ctcctaccgg cccaacaaaa aagtgggctt gcgcccacct
      421 ctcggtgcga cccgcttcag agcgaaaaca gagtatattc ccgctgcacg gtaaaagcaa
      481 gatacggggc cccgccccgg cttagggcgg cgcgacctca atcgtgcctt gaagcccgaa
      541 aaatcctccc ggcttcagcc accgaggaat tagtcttgcc gctcccacgc aggctacctc
      601 gcccaaaagc cggactcggc cctctccggt gctttctaca taaatcccag gaaggatggc
      661 ccacgcaaaa tcgtcttggc gcaacaagcc tgcgtcaccg gccctgaaaa cacctatgag
      721 ctaacttcta gtatttctgg tgctttctca aaggccgcca ctgcgctctt tttttgatct
      781 tcaaacaatc aggacaatcg ccgaactttt gtccggcctg aaactccacg acttcattct
      841 ttacgtcgta gaattgaaag cacctgacgc agcgatactt ccctgtctgc agcacgatct
      901 cgcccgggga gaacccccac acgcgggtca gagtctcaga tagtttgtcg ctgagcgcgt
      961 gttgcgcgct cagcgtgagg gaggcaaccc tatccttctg ctgtactgac tctcgacgaa
     1021 tcgagaagca ctggggtagg aaaagacaac ccgtctcgaa atagccgcgg ggatgccttg
     1081 gcattcctag gagactggca tactttccgg ctaacaagtt tccttcgtca atattggttc
     1141 cttcgattgt gattagggga cagaccaacc actgcccttg atcgagatca caagtctgcg
     1201 tgaggatggc agctagtcct cgttcagcag agacggctat atattcaggc acgtcatcga
     1261 acgcattcac caactgctcg gcggacgctt gcaaaaggcc gggtttcggc gtccacggtt
     1321 tgccatctgt ttcacgcagg ataaccaagt gaggacccgg aggtggactt atcaacggaa
     1381 cattcgggaa aatgtcgcct tgctgaggat aagggggcga ggttagatca tagaaatcct
     1441 cgcccggatt gctcaatttt ctacctcgta tttttgaaag cgagtagcct gtcacgtggc
     1501 ccgtgatagc gctcgcctgc tgccgccgtc gtacccgcta ccgacttaat aggcaaatct
     1561 tcttcgcaag cgacaatggg gcgattcgta attcgatgtt cgtggctaac agcctggaaa
     1621 gcgtccaaag aactcggcgc ggacgcctgc attgtatctg ccgtgaccac gaactcatct
     1681 tctttgaact ccatgtgagt caatgctggc gtcgcctcac tggcgaacgc cacattaccc
     1741 cgcgttgcta gtgcgcccat gctttgcctc cggctgtttc tgaagaatct cttcaatagc
     1801 acggaaaatc gtttcgaagt gtcctttgag ttgcaacacg ttcgctgctg gcatcacaac
     1861 cttcgccaca gtctttccct gcaactcgag gatttccggt ttctcctcgc tcttgatctc
     1921 ttgcatcatc atagcgacct tgttcaggtc gaggaaaaca aactcgaact gtacgtcgtt
     1981 tccgacctgg gagacagcaa gaaagttgct gtagagagca ggaacagcgt taaccggctc
     2041 ttcaccgacc actttgaagt ttagcacttt gggcttcttt gcagacactt gtgcctcagc
     2101 acttattcga tgcaagctgg gtaccgaacg gatgcgtaaa taactccttg tattccatgc
     2161 acttacaatg ccgagatgga gacccctagt tccagtgtga aaaattatct catttatgag
     2221 cgctcccaag gctccgatga gacggggagg tttcacctgc ccttcgactg gccggaagga
     2281 caggtcgctt tccgagtatc ttcatattac atgttcgccg cgcgtcgcaa gagaaaaagt
     2341 gacgtgcggc gggtaggcac gctaactgag cgaataggtc cggcctggct tacctttgcg
     2401 gttttggttt tgcaaaggtg ggacattctt ctcttggcgt gcagggtctt ctacttttcc
     2461 tctattcttg tgcgctcctt tttttcacaa gtttttccgc tttcccgccc gggcccccat
     2521 cccctgaact tcgctcatca acccctcaac ccccagcctt ttcaacacct tcccgctccg
     2581 ttcgaatgat actcaacaaa tacattgaca cgcggtgtat gactatgcta ttctttcccg
     2641 tgtttgccaa gccccactct cgccggcctt ttgattcttt ctctcgccta atccctacac
     2701 cccgccccct atcacctaac tcccatggaa tcatagcgtt taccgacccc catcccttaa
     2761 actctgtcat atcatatcgt tacaaaaaca taggggggca gggggtgcct cccgaaggca
     2821 acgctagcat tgcccaaaat accgtcgcgc atccaccttt cttttcaacc acttgcgcga
     2881 tgctcctcgc gcaacccctt tcttttgata atcttccatt ttcatggggg gtatgggggt
     2941 ctctgcgcct tttcgacgtt ccgacttcag atccgcaaac gtttcaactt gtttccgatc
     3001 tatcctcttt cctttcatac tcttgcacac tctcttgcac tcatcaaaat aacaactctt
     3061 ttgttttcaa gcacttccgc actctttgcg caaaacaccc gggggggagg tacagcggcg
     3121 tggaaatcga aattggaaaa tagaaattag agaggggaga agagcaccaa tcatgccggg
     3181 aatgaatgaa ccgggtctac ttgcgctcgg caggtactcg aatggcgtca ttcatatccg
     3241 tgttggcttt cttgcaagat tcacgctcgc cttgataaca acgctcaagg tcctccagat
     3301 agttttgaag aaccctcgcg cgaatcttgc tcaggccctt tacgcgagcc tcgtccaatg
     3361 cggccttcgc tttctgctcg gtaactttgt ctgcatgttc gccgaactgc tcgatggcgt
     3421 tgaaggtgct ccagtaagtc cggtcctgcc gctcacgctc tcgcgtaatt tgctggttca
     3481 gggtgtaaac caagagatag ttggctccca agacgaacac cagccccgcg atcccaagaa
     3541 tcatgagccg cttgaacatg gtgcggagcc taccgcatac ccaaagttcg tactagcgga
     3601 ttattctaca aagtcgagag tcgagaaatc agagttagtg gggtgccagc gtcgaggaca
     3661 aactgtcttt ggcggcgttt tcgtagaggt gcaggagttg cgcggcgcgc ttgagcaagt
     3721 ccgcgggcag gtcgccgggt tgatagtcca cccacccggt cgtgtagaca agattcattt
     3781 tgcggccgga acagaccatt tcgagagcgc cgagccggtt caagaccgat ttgacttcac
     3841 ccaaattgca ttcgggcagc accagcagga aatcgtcgtt ggccaggcgg gccgcaaaat
     3901 cggagccgcg acacgccttc ttcaagcgcc gggcaagctc tttcaaagcc gcatctatgg
     3961 ccgacggacc gtagcgatta ttgatttcgg caaactcgtc cagatggacg gtggccaggc
     4021 tcaacgccgt attctgccgc cgcgcgcggg caatctcttt cccaagctgc tgctcgatga
     4081 aagctcgcgt gtgcaagccc gtgaccggat cgatgccgga ggaatcggaa atttgaccgg
     4141 actgcgcgtc cgcgccggca ttctgcccgc tcagctgctt tcgctggcgg cggaacgacc
     4201 attgacggta gagcatccag ccattgaaaa gaagaagcag acacaggatt ccccaacgag
     4261 cctgatcgga acggatttcg tagaaatgct cgctgtgccg gaaaaaagag gggaaggagg
     4321 agagcacaaa agccagcgcc gagagcgtgg cgacgaaaaa tgcggagaac caaagccacc
     4381 actcgcgccg cgcgagttgc cgcaggccgg attccgcttg ctgtgtgcgg aaaggttccg
     4441 atccctcgag aggcgaggaa ttgggttggc cggcgcggag accgagtgtt gattccatgt
     4501 tccttacggg aagaccgata caccaccaca ttaggctgct gctgcaagag aaggcaacgc
     4561 cgaaatggta cagggccgtt cactgcgaga acctgaccgg agggtcagaa tgggggtggc
     4621 ggtaagtata gccgtgaggc cgcggcaaaa gaaaaacgcc cccggcaagg ccggaggcgt
     4681 tttgacgaag ctcagaatcg agcgagaaaa taagcgatcg ctagggcttg gcagcgggcg
     4741 gctgagcggc ggcaggcttc ggtgcggccg gcttgggctg cgtcgcggcg ccggccttga
     4801 ccgggtaggc ctgatcgaag aggcggacga tgtcctgcgt tacgtcgatc tgcgtggagg
     4861 cgtagataat cggagtgctc tgcgccgaag aatccagaac gacggaataa ccgttttcgc
     4921 gggcgtagcg gtccagcacg tccaccatct tgcggccgat gcggtcggtg atatcgccca
     4981 gtgcggcgtt tacgtcttcc tgatattcgg tgttcatgcg atcgagacgc tgcgcgagcc
     5041 tctgaccctg ctgcctgagg cgggcttctt cttcctggct cagcttgttc gcgccggctt
     5101 ggaggcgctg ctgcagatcg ttgatctgct tgttcaaatt ctcgatctca ttctggcgcg
     5161 gagcgaactg cgattgcagt tcggcctgtg ccagcttgcc ctccgccgtg ctcccaatgg
     5221 ccaggcgaat actgatgacc ccgacctttc ccgatgcggc cgagccggcc gcggcgggcg
     5281 cctgcgccca agcggctgcg gtgctcaaga tgcatgcggc ggccagggca actgtaccag
     5341 tccacatatt tcgtgaccgc aaaacggtca acatattccg tgaccgcaga gcggtcaaca
     5401 atctagaatt cacttttttc tccttgttgt ggtccgccac ggaaagaccg ggacgaaccc
     5461 gcgccaagtc agcttggctt aaaaccgatt ccgctcccag cgggctttac gagcgtattg
     5521 caaacttcaa agtatagccg acgcggtttg cttcccgcaa tcagaaagca agatggcccc
     5581 gaaagccccg gcagtaagac ggctcccgca tgaagagctc ttcggtgctg ccatttattt
     5641 gggcagcggg aacaggatca cccggttgtt ggcctgatcg gcaatgaccg ccaggttcgt
     5701 aaaggtctgg atggccgcag caaatttcga tgtgccgcca attccgagag ttgccttggt
     5761 cgcgaaggtc tgcgaatcca cgatcgagat cgtattgctc agcgaattca ccgtgaggat
     5821 tgtgccggtc tggaaattgt aggcgatggc gttgggattg atgccaacct tgatcgttgt
     5881 cgtgccgccg gtgtccggat tgaacgcggt gatcacgttg ccttgcgtgg aaactgcata
     5941 aaagagcgcc ggagagaccg aaggatcaaa gaccaaacca gtgggggtac tggaaaggaa
     6001 cgatgtcgag ccggaggtgg atctgaccgg cgtggccaaa ccgatgtcca cggcatccag
     6061 agcaccgaat ggggaggagg cgccgttgag gacgaggcag gtcacgacgg ctaggcctcg
     6121 gccgttggtg ccgcgatcgg ggtcaatggc gacggcgatg gggttctggt caacagccac
     6181 actggtgggt gcaagagtgc cgatggtggg agtggcggtc agcggtgtga gatcgatggt
     6241 ggaaacggtg ttcgaaccgg tgttggcgat gaccgcgaga ttcgtttcct gatcgatggc
     6301 cacgccgctg ggaccggtgc cgacattcac ggtgatgggt tttgcggaag cgtccaagac
     6361 ctgaacgcgg ttcacgaggt caataaggga gacggaattt gagccactgt tggtgacgac
     6421 ggcgacgccc gaggtgccgg cggcctgtcc ggtatacgcg agcctgggaa gaacagcgac
     6481 gccagtgggc gtatcgccgg tggggatggt cttgacggtc ttattgagaa tgttcgcggg
     6541 gttcaggctg aagacggaaa cttgattgca gcccgcgccc gtattggtga cgaccgcgag
     6601 attatggact tcgtcgatgg ccacgcctcc cggagcggcg ggggtgccgg ctgaggttgg
     6661 ggtcacgttg cagggatcca aaagaatttc ttcgacaacg gtgaagtcga cgacgttcga
     6721 agtgacggcg gcgctgttga acacatccag cgcaaagtgg tgaggaccgg tgaagaagtt
     6781 aggaggaaac gtaacgtcga gttcctgact gcctttcagc acgaccgttg cgccgagggt
     6841 gaaaacgtca atcgtatcga gacggacttt cgaagagccg ctgaaaccag agccgaaaat
     6901 cttcacgggg ccagtggcga cagaggtagc tccctgggtg attttgatgg cagcagtgag
     6961 gttcgcctta accggaatgt ttttgtcgtt gggatcatcg atgagcggag cgataacgtg
     7021 ttcgatgtgc agggccttaa tggtccctag tgtgaagaac gagatattgt cgctgccgga
     7081 attgacggcc agcgcaagat tgtttgtgga atcggccgcg agggctccgg gcaggttgac
     7141 ggtgatggcg gtgctgctcg ggccagcggg agtgaagctg gcggttgctt tggcaacttg
     7201 gccagggccc gtggaaataa ttttgctgcg ctgcaagaga gagggatcca gaagagaaat
     7261 ttggttgaga ttcgaattga aggaaagcgc ggtattggtg aacggctgga aagcaacggt
     7321 agccgcgccg agttcgggag taccggtcat cgtaccggtg gcgccaacaa acacggtcat
     7381 gaagctgacg gcctggctta tcggatcgat gaaattgatc tggctggagg tggcattggg
     7441 atccactagg atggcccggc gcgtgattgg attgatggcg atccccagcg tgctgggcga
     7501 aaccgcatag gtcaggaagg ggacgccgga gctggcgaga cagccggtgg tgcaggtggc
     7561 agtgtcggct ttgtcagcct gggaatactg gaaatgtgtg gcgtccagaa cattgccgac
     7621 cgaaaaagaa ccattgaaat tggtcttgtt ggtgctgccc aggggcaagc cgctgatgag
     7681 cactgttcca gggttgccag gattgaggtt gtgagaggca gacgtcgtta tggtgactac
     7741 gttggaggtg tcgcgcgtgg cgctggcgac gccgaccgat ccgaccgagg gattggcgag
     7801 gttcacggca gagagctggc ccgcgccacc gggggtcgcg tacgcgagat gagcgccggc
     7861 ttcgaaagca atttgaggat ttgctccgct gttcagcgtg acgtaggcaa tgggacaaaa
     7921 attcggagtg gccgtctgtc cgggcaaaat acagccgtta ttcgggagga cgctggagct
     7981 ggtgtccaaa ttgatgatga gcccgacgtt ggtggaagca aaggccacca acgcgtgatg
     8041 cgaaaaaggg tcgatgccca cagagtaggg gaatggcgcg acgaacgacg aaccggacgt
     8101 ggcaggcggt gggattacgc cactcaaatc cactgttcct agcaacattc ccgaggggat
     8161 ggagtgaacg gtgagagacc ggcttgcgta attcacgacg gcggcgacgt gcaactggtc
     8221 atctacagca acgccggtgg cgagaaaccc ctgcgaagaa actaacgcgg tcagagtagg
     8281 cgtgccacta gccagattga tgaactgaat gttgttggcg gtgttgaccg tgggagcagc
     8341 agttagaaag gcgttttggc cggccagagt gacaacggca taccccttga cggaatcgag
     8401 ggcgatggcg ctgggcatgg tgggcgaggc gggcacagca ttcccaggaa tcgcgagttg
     8461 cgaaggcgcg cctgggggat tgtttccggc gtagtctggg attacggcga tattggtgta
     8521 ggcggtggcc ggagtgggta cggcgaaagg ccccggagat gtcgagtact tcacgctaag
     8581 agggaacaat ccggcattgg gattagtggc gctaggagtg ggcaagaagc cggtgagacg
     8641 tcgcgacgtc acactattag gctgaagcag cgttgatgat gaagtgttgt tgaattgagg
     8701 agtgatcgtg ggtgaaaccg tgcatgaggg cagcgtgccg atattggtgc cgaaataccc
     8761 tccatcgatt gttatcccat cttgagctag cgtggactcc tgaagatttt cgggaacgga
     8821 acttacaatg gtgggccgaa cgggaatgat atccaacgga aacggcccgc cggtaacctt
     8881 gacggaaggg ttgctgccgg tgacttgcaa ttggtagtgc cccgcacccg tcaattgagt
     8941 tttgttgagg cgaatgcgag cgccgatgaa agaggaagtc gtgccggggg cgaagattac
     9001 cttgacctga gattgttgat cgacggggat gtctccacag ggtgcagtga gcgtgacgcc
     9061 gagttgagag gtggcgttgg tagcggcgag gaagatgtcc tgctggagag cgccctgggg
     9121 cacaacagac ggcgaaatgc cgctaaaggt gatgtcgcca gccttgacta tggtgatagc
     9181 cgcctgggca atgcgcgcgt tgtcgacatt cgagaaaacg aaaagggtca cgatcccagc
     9241 ggcattgacc ggagtggtcg tagaagccgg cgcagccact ggaacggcgg cgggcgcggt
     9301 gtacttgccg gtggtcttgt caaccgatcc gcagtcgttg cttccgccgt tgcacaaggc
     9361 gctgttggtt ctcgcggtgg attcgaaagt cacgccccag gtgagttggt cgggcttaat
     9421 aatagtgccg ctgaaatcct tggcgagaaa gagctgcgtt tcgacggtgc ccagagtggc
     9481 ggttgccggg ctgagcgtga tgcggatgcc ggaatcgaaa gcaaggctga acttgcccgt
     9541 ctttttcttg tcggcattgg cggtggccgt gatggtgacg atgacgttgg ggagcttggt
     9601 ctgatccgga aaaaccttcg gagccgtgaa cgtggccgtg gaggcgacgg tggtgctggt
     9661 gctcgcaata ggggtaagcg tgccgacggc gggatcgccg ctggcggtct tggcagtatc
     9721 acaggccgca gaagcagaag gcttgggact cggaattgcg gtcgtgggat tctgcgtggt
     9781 ggtgtaggag cagtcgaagg ttgcagacac atcggtcgcg ccggtgacgt tggcgatgat
     9841 cgtctgcgct tgcgtcggca ccatcactga gaacgagccc aaaaccgaga caagcacctg
     9901 gttggcggcg ctcttgctgc cgcacccgct gaccgcaagg ctggcgaacg ctatcgccag
     9961 caggatgttt ctcgcactct tatgccactt catacccact gtctccccca catttgcgcg
    10021 ctttgcaagt gtttcgcctc ttcggcgatg ctgaattccc aagtctttcc gaaaatcaca
    10081 attttcctcg caccctagaa cgtccggctg acggtgaagc ggaacgtccg cgccggctcg
    10141 aaatagttaa gacgccctgg attgttctgt aactgcaaga tcgtaggttt gagctgattg
    10201 agccatacat ccggcggcag ggtcctcggc aacaaaccaa tcggcaaacc gtccgaaccc
    10261 aatgtatcgc tgatctcgct cggattcagg aaatccggag gggcaacgat ctgcgaatgc
    10321 aggcggttga cgttatacgc gtagtagatc cggaaaggcg cctgaataat cgggagttgc
    10381 acaacgaact caatgccagt cgagcctcgc aggcggaaat tcgttcccgg tgcaatggag
    10441 agctgctgag tcaggcccgc caacttcctg gcatctggga attgagtttg cagattgcta
    10501 aagccggtcg gatcaagctt caaggcgttc ttgcggagaa tgccgtttgt tccgccatca
    10561 aagaagagcc ccacctgcac gatgttgccc acgatgggga tacgatattc aacgtttcca
    10621 tagctctgaa ggtcgccacc ggggaaagaa atggtatagc ccagcaccgg cacgttgacg
    10681 gtcctctggg tcggctgacc gaaaccgttg agacaggttc cgcaaaggta gctgaactgc
    10741 tgcgtcgagg cgacgggaat aaacgtgacc ggtgtgatgg agcggatatc gaagccgcgg
    10801 atgtcgctct caccacccat gtagaaccgg ctgtatggcg gcggttcccc gcccgcgtaa
    10861 ccggtgatga acgcaccgga agcgtgaaaa cccaaaacgt tgcgccgctt gttcaccgga
    10921 tggtagtact tccaatcgaa aacgttggtg atggtcttga cgttgccgcc gagccccgca
    10981 aaccctagcg aatagaagta gctcttgccg ccggtgggat tgatcggatt gttgacggtg
    11041 ttataagtga ttgtcggcgt gatggcgctg gagacgattc cgttgagcgc gctgggaccg
    11101 gcgacgctgc ggaattgcaa ctgcgtgaac agcagcttgg aagcatcgtt aaatgccgtg
    11161 atgttcgtgc gcgtcagacc atattgcagc cctattcggg tgaacgagag ctttcggacg
    11221 ggatagctgg cgaaaaccgt gacgccggtg ctgtcctgat tgtagttctg aacgaactgc
    11281 gggttgatgc tgacggattg cccggtatag agagccgcct gccgtgcctg gtcaaacttg
    11341 tagcgactgg aaaaaatcgt gaaacccgag gagatgggcc ggtcaaagag atagggctcg
    11401 gtgaacccga acatgaagct gcgcgaaaga tcgccgaatt gcgccgaaaa ggtcagagtc
    11461 tcgccaagac ccaggaagtt gttggtctga taggtgaggc cgatgaagct gccggccagg
    11521 ccgctgacgc cgccctgcaa gccgatcgac tgtttgccct tttccttgag cttgagattg
    11581 atgtctactg tgccttcttt actattgcgc ttgatctccg cggccttgtc cgcttcgatc
    11641 ttgtcgaagt agtcgagctg attgaggcgc aggatgctca tttcccacaa gcgcttgttg
    11701 aacagctggc cttcatcaat ggccaattcg cggcgaatga ctttgtcgcg cgtggtggtg
    11761 ttcccgctga agtcgatgcg cctgacgaaa tactgcttct gctcgtcgaa ccgcagcgtc
    11821 aggttaatga ttttgttcac atcgtcgact tcggtgtccg gctcgggagt gaaatcgatg
    11881 aatccatatt cgccgtaaat tttgccgtag gtctccaggg ccttgcgaac tttttcggtg
    11941 gagaagaggt caccttgctt gaggggaaaa gcgcccttca gcgcctcgac cttgagggac
    12001 aaggctttgt ccggatcgga gcttacgatt ttgagagtgc ccatgcggaa gcgctcgccc
    12061 tcttcgatcg ggatggtgat gttgacggcc ttgccgtggc ttcgcccggc gatcagcgga
    12121 acgcccaggc ggtaactttc ggtgtcaata ttttcgagaa ggggttcgcc gacggaaacc
    12181 ttgaagtaac cgttgtcgcg atataggccg cgcacgccga cttccaaatc ctcgctcaac
    12241 ttttggcggt cgtaggtctt ggtgagcacc gggatatacc agaagtacag aggaatcgag
    12301 tagggccggt cgtggcgcat cgcgcggatc aacttgcggt cgctgtaagc gtggtttccg
    12361 gtgaatttaa tcttgccgac cttgaccttc gggccttcct cgatcttgaa aacgagaatg
    12421 acggcgttgc tggaagcaat gcgctcgtac tgcggagtga ctttcgcaaa ctggcggccg
    12481 tgttcgccca gcaaatcctt caacacgacc tctgctttct tgattttcgt gggatcgaac
    12541 tggctttcga cgctcagacc gactttgcgt tccttgaacc gatcgagaat gtccgattcc
    12601 gatacggagt ggatgccgtc gtagcggatg cggcggatct gcgggcgctc tcggacctca
    12661 aagataatga ctttggcgtc ggtgcgcttc gctgaatctt cgacgcggag tttcacgtct
    12721 tcaaaaaact gcgtattcca gagagcctga aaatcacggc gcagcgtctc ttcattgtag
    12781 acatcgccgt cgcggctgaa gatgcgcgct ttcagcgtgt cgctgcgcac gcggcggttt
    12841 ccgatgaaat cgatgcgctc gatgacaaac tgctgctgtc gcgcggcagc ctgcggatgt
    12901 gccgcggaac ggtcatctat aacgtctttg ccggtagcgt aatcaaaata aacggtgaaa
    12961 aggtaggaat gtgccaatga ggatgcgtcc gcatggggat tttcccgcgg cgctgcgggt
    13021 gacgcgatcg caaaatgaaa caccatggcg atcatccacg tcacgcgagc cgcaaaaaaa
    13081 ccaaacggcg aacaaactct tctttgagcg gccgatgagg ttgtcaaggc cgcgcccccg
    13141 aactcaagtt gtttttctcc cgccggaccc ggtgtgggcc agccgtgcgt tgatcccgac
    13201 ccggccggga tcacccgtgc gttgtgtcag attcgctagt ggacgaccgg ggttgcctca
    13261 ccgacggggc ggaacgacag gccttcccct gcgacgtaaa tctggagagt cgccggcttt
    13321 tgaattccac cctggatcag cgcctcggag agcgggtctt ctacatactt ctgcaaagcc
    13381 cggcgcagcg gacgtgcccc atagttgcgg tccgcacacg ttttctcgag tatccactgt
    13441 cgtgcttccg gcgataccac aatctgtacc tgccggtgaa tcagcgtgtc gttcagctgg
    13501 cccaccagca aatcgatgat gcggagcaga tcttcgtcgg cgagcggatt gaaaataatc
    13561 acttcgtcca gccggttcaa aaactccgga ttgaacgccc gctttacttc gttcatcacc
    13621 atatcttcca tgctcttccg tccggcttca tccgcgccgg actggaagcc catcgacgtg
    13681 cgcttttgca aatgacgcgc gccgagattg gacgtcatga tgatgatggt gttccggaaa
    13741 tccacggtat tgccaagccc gtccgtcaac tgaccgtctt caaatacctg caacaatata
    13801 ttgaagacgt ccggatgcgc cttctcgatt tcatccagca ggatgaccga gtaaggcgtc
    13861 cggcggacgc gctccgtcaa ctgaccgcct tcttcgtagc ccacgtaccc tggaggcgcg
    13921 ccgatcagct tcgagacgga gtgcttttcc atgaactccg acatgtcgaa acgaaccagc
    13981 gacttctccg aaccaaacaa aaactctgcc agccgccggg cgacttccgt cttgccgacg
    14041 ccggtcggcc cgaggaagaa gaagcagccc accggacgca aaggcgactt gagacccgcg
    14101 cggctgcggc gaatggcgcg cgccaacgcg gtgatggcct tgtcctggct gatgacgcgc
    14161 ttgtggagtt ccggctcgat gcgcagcagc ttttgctgtt cgtcttcctt gatggaggtg
    14221 acgcctatcc cggtccagcg cgaaacgact tcttcaatgt cttcgcgcgt cacgatgccc
    14281 gtggaggcgt cgtccaattt gaagcgctcg cgcaacccgc gcagattttc cttttccttg
    14341 cgctcttcct cggaatagaa acgcgccttt tcgaattcgt gattggcgat ggctgtttcc
    14401 atgcggtggg tgatgaattt cacgcgcttc tgcacttcgc cgacttcttc ggggacggtg
    14461 gcctggcgca gcttcacgcg cgcgccggct tcgtcgatca ggtcgatggc tttgtccgga
    14521 agaaaacggt cggggatgta gcggttcgaa agataaacgg agtagcggag cgcttcgtcc
    14581 gtgtagctga cggcgtgaaa tttttcgtag cgctcgcgaa taccttggat cacctgcacg
    14641 gcttgctctt cgtccggcgc gggaaccttc acggactgga agcggcgctc caaagagcgg
    14701 tctttctcca ccgacttgcg gtattcaccc ggcgtcgttg cgccaatgca ctgaatctct
    14761 cctcgtgaga gcgcgggctt cagaatattc gcagcgtcca gcgatccttc cgcggaaccg
    14821 gcgcccacga gcgtgtgcag ctcgtcgatg aacacgatgg agttctggct ctccatcaat
    14881 tccttcatga tggtcttcag ccgctcttcg aactggccgc ggtacttcgt gcccgccacg
    14941 atcagagaaa gatcgaggga aaggatgcgc ttgtccgcca ggaacggcgg aacatcgcca
    15001 tcggaaatgc gctgcgccaa gccttcgacg atagcggtct tgcccacgcc gggctcgccg
    15061 atcaaaaccg gattgttctt ggtgcggcgg cacagaattt gaatgacacg ctcgacttcg
    15121 ttttcgcgcc cgacgagagg gtccagcgag ccttccgatg ccgctagcgt gaggtcgcgg
    15181 ctgaattccg cgagcaaaga cgtttccttt ggccgcgccg ccgccgtttt ttcaccggac
    15241 gtgcgcgcca gttcttcccg cagcgtggaa agccgcaggc cgcgctccat caaaatctcc
    15301 gcgccgaagc acttttcttc gcgcaggatg ccgagaagca gatgctcggt gccgacgtgc
    15361 ttgtgcccga ggcgctccgc ttcttccgcc gccatattaa ggatgcgctt gcactccgcg
    15421 ctgagcggca cttcgacgga ggtcgagatg cgctcgcgga tggtgatgcg ggcctcgatc
    15481 tccttacgta tcgattcgat cgagccttgc tggcgcagga aacgattggc cagggctttg
    15541 tcctcgcgca tcaagccgag cagcagatgc tccgtctcga tgtacgggct gccatactgg
    15601 ctggcttcgt accgcgcaaa aaatatgacg cgtcgcgctt tctctgtata acgttcgaac
    15661 acgttagctc gcttctcccc ctggggcccc gctcgcggag gccgcagcct cgcgagttcg
    15721 cagaacgcca tgctccagcg ccagtatccg gtcacaccgc gcggcgagcg ccaggttgtg
    15781 cgtcgcgatc agcgacgtca acttgtgcgt gcggtgcagc cgttccagca actcaaacac
    15841 ggcccaagca ttcttctcgt ccagatcccc ggtaggttca tctgccagca gcactgacgg
    15901 cccggtgacg agcgcccggg caatcgcgac gcgctgctgt tcccctccgg agagttcccc
    15961 tgcgcggtga tcgactcgat tttccaagcc aacctccgcg agccacttcc gtgccatccc
    16021 cagcgcggaa gcaaaatttt cgccgcgcac cagcagcggc atagctacgt tttccgcggc
    16081 cgtaaaatcc ggcagcaact ggtggcgttg ccacaaaaat ccgatggcac gattccgaaa
    16141 ttccgccagc gccgcgtcat caatcgattc aatggctttc gttgcgaagt atactgtacc
    16201 gctcgttggc gtgtctagcg cggccagcag gtgcagcaag gtacttttgc cggctcccga
    16261 tggtgcaacg attgccacca tctctccctg ccgcagactc aagtttgcat ccgccagcac
    16321 cgagatggtt ttctcgccgc tgccgtaagt tttcttcagg ccgcgcgatt ccagaacaac
    16381 tgccgcgttg cgcagatcct ctgccggctt cccgctcgtg aattgtgtct gctggatcat
    16441 cctgctgcta cgcctttcgg tctgctcaca cgttgttcct gctacttact tagatgcttc
    16501 tgctgcaatc ccagtgccgt gcggtttgcc cgggcactgt gcttcagatg acccaaagtg
    16561 tttcccattt ccgccagtct agtacctgcc gaaaagaggg caattctgcg tcaatctgaa
    16621 acgcctataa tctttctgtt ttcaacagct tgcggcggga tcttgctacc accgagggtt
    16681 gagggaagcg ccccggtctc acccacctgc ctctcagctc tgataggagc aaatctcgcg
    16741 ccaattggac cccagaatat tgttattgcg cgcgattccg gctcttcatt cataccggag
    16801 aatttccacc ggcaggagcc gcaccgcggc gcgagccggc aaaatcgttg ctgcgatcga
    16861 aattcccatc gctacggctg tgatccacac cccatcgaaa acactcgggt gaaagggcac
    16921 ataggggacg gcgtacacct gcggatcgag aggaatcaga tggtagttcc ctgccacaaa
    16981 cgaaaatgtg tagccgatga tgaggccgat cagcgtgccc gcggttccaa tgctgattcc
    17041 cagccaaaga aaaatcgttc gaatctgctc gcggcgcgcg cccatggacc ggagtatggc
    17101 tatgtcgcgc gctttgtcgg tgaccgtcat ggaaagaacc accagaatgt tgagaccggc
    17161 gacgaaagta atcagaccga tgaagatcgc cgtgaccagc ttttccagcc gcagcgcccg
    17221 gaacagcgcg cgattttctt ccatccaggt cgtcgctgaa taaccttgtc cggcggcttg
    17281 ccgtgcccgt tccgcaattt cgcccgcgcg gtccggctga ttcagacgaa actcgaggac
    17341 gttgacgaca tcacccgcgc cggagagtcc ctgcgccgcg gccagcgtta tgaaacacca
    17401 gttttcgtcg taatcgtaga agccggaatc gaaaactcct gcaacacgaa accggcgcgt
    17461 ccgcggcatc aatccgaagg gcgtcaatcg tccctgcgga cttgtgagcg tcacgtaatc
    17521 gcccggagag actttccact cctcggcaag ctgttttcca atcagcagcg cgtcaatgcc
    17581 atccccgtct ggagaaaagt ctaggcttcc ggcgacgatg cgctgcaggg cttcgtcgct
    17641 ctttcgctcg cgctcgggat cgatgccttt cgtgaccacg ccgcgcgcct gcccggcgaa
    17701 ggaaagcagc accgtctggt agaccgccgg cgtgacggag cgcgcgcccg tcgcgccggc
    17761 gagctttgtg gccatttcct cgtagttctt gattccgccg gtgccgggtc gcgtcagaga
    17821 aacgtgggct gtcacgccga gcaaccggtc ttgcagcgtc tcgcggaaac ccgtgttcat
    17881 ggagagcgcg atcaccagcg tcgccacgcc cgcggccact ccgatcgtgg agaacagcgt
    17941 gaccagacgc agcaccgccg gccggtacgg cgaccgcagg taccgctgcg cgacgaacca
    18001 ttcgaatcgc atgctagttg gaagaatacg ttaggcgccg tgcgcttcgg aagatgattc
    18061 gccgccgcct tccggacgca gcagtggaaa aaggatgacg tcgcggatcg agggcgaatc
    18121 cgtcagcagc atcgtcaagc ggtcgatgcc gatgccttcg ccggccgtcg ggggcatgcc
    18181 gtggcagagc gcgcggatgt aatccacgtc gagctgcttc gggacttcgt ctccgccctg
    18241 ggccatctgc gcaagaaagc gccgctcctg ctcggcaggg tcgttcagct cggagaagcc
    18301 gttggctact tccatgcccg caacgtaaat ctcgaatcgt tccgtcagcg atggatcttc
    18361 cggcttttgc ttcgaaagcg gcgagatctc cgtaggaaaa tcgtaaagga ttgtcggctg
    18421 gacaagtttg tcttcggcca tggtctcgaa aaggagtcct gtccattcgc cgtccttcag
    18481 cgtgcctttg gctccggcgt aaggcgcgcc agcgcacttc gcccagagat tatagcgact
    18541 tgtcgcttcc tgagcgccgc ccggcgagcc caatttttcg gatgtgggcg cctcgcccgc
    18601 agccgcgggc caatacttga cgatcgcttc gcgcatggag agccgctgca ttttcgaaaa
    18661 atcaagctcg gcctcgccgt acttcaccgt ggtcgagccg gtgatgttct tggccagcaa
    18721 cgcgaagagc tgctcgttca tgtccatcaa atcgcgataa ttgctgtagg cctcgtagaa
    18781 ctccagcatg gtgaattcgg gattgtgctg cgtggaaatg ccttcattgc ggaagttgcg
    18841 gttgatctcg tacacgcggt cgaatccccc gaccgtcaac ctcttcagat aaagctccgg
    18901 cgcgatgcgc agatacagat caatgtccag cgtgttgtgg tgcgtcgtga acggccgcgc
    18961 ggccgcgcct ccggcgatgg gatgcatcat cggcgtttcg acttcgatgt accctcgtgc
    19021 atcgaagaac cggcgcaatt cctggatgat tcgcgcgcgt gttacgaaca cctggcgcga
    19081 cttttcattg gcgatcaggt ccagataccg ctggcgatag cgcagctcga cgtccgtgag
    19141 gccgtgccac ttctccggca gcggcagcag cgctttcgaa aggaagaaaa tctcctcgac
    19201 ccaaatggac agctcgttgg tcttggttcg aaacaggtgt ccgcgcaccc cgatcgaatc
    19261 gcccaggtcc agcagatgaa acagttggaa tcccggttcg ccgacgacgt ctttctttac
    19321 ataaatctga atgcgcttcc cagccccttg caggtgcgca aaaccggctt tccccatcag
    19381 ccggaaagac acgatgcggc cggcgacccg cacctcattc ttggttgcct ctaattccgg
    19441 gccggaggtc tgactgtact tctctaccag tgccgccggt gtatccgtcc agcgaaattc
    19501 ccggggatac ggatcgtgtc ccagcgactg gatctgttcg agtttcttct ggcgctgttc
    19561 aagctggtcg cgcggctcaa attgcaaagg gaaaatcctt tcatctgctt tgtcaggcga
    19621 gtatagcggc ccgcgtcatc cttgagcaag gaatgggcct caacatccac cccggcgaaa
    19681 tgaaagcggt acctcgggta ctagcacctc gcttgcgctc gctgtgacgt ccatcacaag
    19741 tcctgatgaa tcagagtcct ggaatgagaa taatctggca tgtggaaaag tttttgctat
    19801 actcccaaat ctgtcccaag ggagatctcc cgtgaaagac tctgtccgcc ccccaacccg
    19861 cggcaagtct gtcaaagacg actggcgcgc gtggcttcca gaagaaaagg cacaggtttt
    19921 cctcaagcag gtccaacaac tcgaatccgg ctacgccatg ttgagcgttt ctctcgacga
    19981 ggctctcgaa ttgaagcact tgggacactc cggcaagtcc ctgcaggcta tgggcataac
    20041 ttcgggcctc tgcaggttgt tgacccagac gctagccggc ttgcttcgtg ctttgtccga
    20101 gcactccaag catttcggaa ccattcccaa cactgcgccc ctcgatcctg ccaattttca
    20161 gggacaaagg ggccagcgtt cggcacggat gagcagcttg ctcagccacg tgctgctctc
    20221 ccatcggctc cagttcctcc ataaagtaag cacgctggag gaaatggtcg aggatcttgg
    20281 caaggatttc cgctatgccg ccgatgacct cgccgaagga gctcccttga atcccaagag
    20341 aatgtgggat gaagtggacg cggaccacta cgacctgaat acctgcttgc gagaggccat
    20401 tgttgtgttc aagtctttct tgattgccct gcccgagagc cagcttggcg ctttccaaaa
    20461 taccgttcgt caacaaactg agcttaggga agtcgacgtc ccttcccgcc accgcctgat
    20521 ccgccataga cgaatgaccg cgatcgcggg agaataagca agcctgctcg cccgcaggga
    20581 tacgggaaac tcatccggga atgagcaccc cttcgggggt gtctgttgaa ggcatgcggg
    20641 cttctgtcag gacaactcaa gggggtattc ggcgcatccg aagtggtgga tgcgccgcaa
    20701 ttggaccgtt ggccagaact cgaccaagcg atcccaatgc gtcgacctct cgtcagtcca
    20761 ccaaaccggt gactgaagcg gaggccatcc gtctggccca ggccggagat gctgcggctt
    20821 ttgaatttct gtaccacttg catggccgcc gcgtctacgc tttgtgcctg cgcatggtga
    20881 ataacccatc cgatgcggaa gatctgatgc aggaagcttt tctgcagctg ttccgcaaga
    20941 ttgggacctt ccgtggagag tcggccttct cgacctggtt gcaccggatg acggtcaatg
    21001 tggtgctgat gcggctgcgc aagaagtcgc tcccggtggc ctcgctcgag gaaatgacgg
    21061 accccgacga tgaaacgggc ggccccagga aagacatcgg cgcaccggat ttgcgcttga
    21121 gcggtgcagt cgaccgagtc aatctggaac gttcggttga aaagttgccg ccgggctacc
    21181 ggaccgtgtt cgtgctgcat gacgtccaag ggtacgagca caacgagatc gcggacatca
    21241 tgggatgctc ggtgggaaat tcgaagtcgc aattgcataa agcgcgcaca cgtctgcggg
    21301 acttgctcca ggaagaggta cgccaaaagg cacggcaagt acggcatgcg gcaaaaactc
    21361 agacggcgca tgatgattga gctacaatgg gcgcaatcga gggctgtgta agcgacttca
    21421 cggcggaaca tcagggattt gcgccgaaac gatttaagag aagtcgaacg gggcaggcat
    21481 ccagcgccag ctcccgcgga gtgcagggtc atgcagtgta aggacgtgga aatcgtagtg
    21541 gagcaggaag gtctggcgcc gctgccggaa gcagcccgag cgcacgtggc cgcttgcagc
    21601 cattgccagg aatttgtcgc cgatctcgcg accgttgttt ccgtcgcgaa tgaattgccc
    21661 ggcgaagtcg aaccgcccgc ccgcgtgtgg gtttctctcc gggcccaact cgaactcgaa
    21721 ggaattatca agacgcccgt cgttcctgcc agcggtgaac gctcatcctg gtggcatggc
    21781 ttcaacgatc tgtttcgcag ccgcgctctg gctacggcga gtgtgggcct gctgatcgtc
    21841 gccgctggag tttttcaatt gcggcaaacg ccggatgcgt ccgtcgggcc taatgggatt
    21901 ggtgaccggc ccccttcgca gatggccttc gcgcaaactg ccaaagttct gaacgagcag
    21961 gaagtcgatc ttaggaacat gcggcttgcc ggcacttctg cctctgctgt ggacgactct
    22021 ttccggcaga acctgcagca agtcgatgat tttattgccg tctgcgaaca gcgtgtgaag
    22081 gttgcgccgg aggacgatct ggcgcgcgaa tatctgtcca acgcctatca gcaaaaagcc
    22141 gaattactgt ccgcgatgat ggatcgcgaa gggagtatcc actaacatgg gaattggaat
    22201 catggggttc gccgctttgc gatcagattt gaagcgcggc atcctgccgg tactcggcgc
    22261 cgctgctttt ctctgctcgg gcctgcctgc agcagctgct gggcagcatc tcgaaaagca
    22321 tttcacggtc aatgcccgcc ccgtggttgt aatccacaac gttgccaacg gccgcatcga
    22381 agtgaagtcc tggaaaaacg ccgaagtcga cgtcgtggcc agccagactt ccgacaagat
    22441 tggctttgat atcgagcaag ccggtgaccg catcgacgtc actgccaata ttctgaacgc
    22501 cgcggcgcaa ccccgggagc tggaagccaa tttgcagctc accgtacccg aagaaacgga
    22561 attgcagctc aagacggaga ccggacttat ttacgtcgag caggtcatgg gcgacatgac
    22621 cctggaaagc gtcgccggcg atgtccacct gaaggaagtt tccggctaca tcattgtccg
    22681 caccacggaa ggttcgctgg tctgcaccca gtgcgccggc aaactggatt tcaagtccat
    22741 cagcggtggc gcgcaggttc tccagccggc gctgaccaac gtcagtctct tcaccacttc
    22801 gggaaatatt ctctttgacg gggatttcgt ccgcaccggc ctctatacca tgaagagcgg
    22861 caagggcctt gtcgaagttc gcttctcggg caacgactct tttgacctca aggcgcaaac
    22921 cgccatgggc acggtcgaca atcaagcgtc cgcgtacctg aagcccgatt cccacggcgt
    22981 caaacatctg gcttcgaagt tcaccaaggg cctcttcggc accgtcggac tgggcctcgc
    23041 caaagtcgaa ctctcctcgt acagtggtac aattcgcatt ctgaagcgcg actagttttt
    23101 cgcgccacga tcgcgtgaca actacacttc ctcttcgcgg gccgcaaccc cgcgtcccga
    23161 ttctgtgcct ggccggtttt atgggttccg gcaaaaccac tattggcacg ctcctggctc
    23221 gccagctcgc ctggcgcttt gtcgatctcg atgaccgcat cgaagcatcc gccggcctcc
    23281 cgattcccca aatcttcgaa cgcctcggtg agtcggcttt ccgccagatc gaagcggatc
    23341 agctccgcgc cgccctcggc cgcgccgccg agatgaaaga aagcaccgtt ctcgccctgg
    23401 gcggcggcac ctacgcgcag ccgggctgcc ccgattttct gcgcgccgcc ggcatccctg
    23461 tgttatggct ggattcgcct atcgaagttc tcctttcgcg atgcatgacc atgaccggcc
    23521 gcccgctctt ccgtgacgaa gccagcttca gcgcgctcta cgcccagcgt ctcccttcct
    23581 atcaacttgc cgattaccgc gtggacagct ccggcgaacc tgcccaggtt gtcgcggaaa
    23641 ttctgcgccg cggcattgtt gcatccggtg gaaacgccgc gcatctgatg gttgagaaac
    23701 ccccgccctg agagagaatg gacacggggg taagaagcac cgtgaaattc agaaccgtat
    23761 cgatcgcact gacactcgga atcgtggcgt gcgccgcgtt gtctctcgcg gctcagaaga
    23821 aagtcgcgcc aacgtccggc cccgctgtct tcgctcaaga taagggcaag ctcaccatca
    23881 aactcggcgg ccagaccgtc ggtcatgagg aatttgaaat cgcgccgtct ggcggcggct
    23941 gggtcgccaa aggcaccgcg gagatcaagc cgcccgaggg tagctccagc aaaatctccg
    24001 gctcactgac gctccagccc gatggcgcgc cgatcagcta tgactggagc gcccaggcgg
    24061 aaaaaacgaa cggcgcgcac atcctcttcg ccaacggcgt ggcaagtatc acgctggaga
    24121 tgcaaggcgc gcgcccgttc caacaggacc tctccttcaa ttcgccgctc atcgccgttc
    24181 tggacaacaa tctctatcat cagtacgccg tccttgcgcg catctacgac tggtccaagc
    24241 gcggcgtgca aacttttccc gtgatcattc ctcaggaatt gacgcctggt tcgattaccg
    24301 tgcaatccac cggctccgcc agcgccgacg gcaagaccta cgagggcctc aaggtcaaca
    24361 cctccgacct cgagattctt cttttgctcg acgccaatca ccgtttgatg cgcctggaag
    24421 ttcctgacgc caaagtctcc gtcctccgcg actagtccaa gctccagcaa aatcttttcc
    24481 gcaaagaaaa aacgcggcgt accggtgatt accgggacgc cgcgtcaatt cgtgctctgc
    24541 cttgaaaaaa acttatcgcg ccagcggccg ttgaaaaatc ggcccgcccg gacgccgcag
    24601 cggatagccc tgcggattac catgggcatg cgagtatgca agcccattcg aatggccggc
    24661 accgatgtgg ccattgccat tcgccgacgc gggctgcggc agccgcgccg gattaccgat
    24721 agggcggagc gtgatcggcg ctgcatgaac gtggtcgctg atggcctgca acctcttcag
    24781 gatggactgc cactgttccc aatctaattg ccgcaggaac ggccgcaatc cctggatgaa
    24841 cggatcttcc agcagcgctt cgccgtcgga ttccgggatg aagaaccggt tcaagccgac
    24901 gctcagcgcc tcggagatct tctccagtgt gccgaggctc ggcgtcatct ggccgctttc
    24961 gatgcgggag aggtaggagc gcgaaacctt cgagcgcgac tgcagttggc tctgggtcat
    25021 ggcacgcgac tcgcgcaact ggcgaatgcg ctggccgata ttctcgaccg tctcctggtt
    25081 aacgaacttt tcgggcgtag gttcagcggc tgcttcctcc ggaggttccg gtggaggaat
    25141 caggaattct aagcgctgag gtaaggcgcg cacgcaacgg cggcaattcc ctgtgcgcgt
    25201 ccggtattgg acgagaccac atgtcttaca gcgaatgact tcccgatcca tttctaacga
    25261 gctcatctct ttttctaatt ggacagacac cggtactcct tgctgactgt ttttccggcg
    25321 tgcggataga gggaggaacg ctggaacgtt cgttcggggg gaagacagtg actggcgcag
    25381 agggagtatc gtgcggctgc cattttctgt caagagtatt tttgaactat ttttacgctc
    25441 gccgtacgcg cttcgccgtt ctacgccctg ttttcgcctg tcctttcggg cagttgtgtc
    25501 tcctcgctca gcaatttccg cgaacccttg tgtagtttct cacaacccac cacacccctt
    25561 gtggtgccct cccacccaca gcttttccac aggccgtgca ttcagccctg tgtttctccc
    25621 cgcctcttgc gcgttggccg cgtttgacct gcgcgaactc gatcgaacgc tccgatcttg
    25681 taggaaacgg ccccgcccgc tcattctttc ttccctcctc tactaaacac ttgaa
//
LOCUS       DASYVO010000309         3312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_262657, whole genome shotgun sequence.
ACCESSION   DASYVO010000309 DASYVO010000000
VERSION     DASYVO010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262657"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VGR03_10525"
     CDS             <1..463
                     /locus_tag="VGR03_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV1994756.1"
                     /translation="THSPSVLGDAITMMGSIGFAMYVVLGKRLAGRYDPLTMTAFSHY
                     AGALIVLPVAIYRAITLGSAEHWRAIAWTGWAAMFYMAIFSSAVAYVFYFWLLRYLEA
                     SQLSAFTYVCRSSQPSWEFYGSAKRAPGGRCSEGSSRSVASIGLNLAGHLS"
     gene            651..2573
                     /locus_tag="VGR03_10530"
     CDS             651..2573
                     /locus_tag="VGR03_10530"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1994757.1"
                     /translation="MRAKLAIAGLLLALVAAGFYWYSQRGMKLSETDTILIGDFANAA
                     GDPVFDGTLREALAISLAQSPSLNLVSVEKVGEALRSLGRPVDAPITRDLAPKLCQRL
                     GATVYLTGSIDKDGSGYSVRLDAARCSTDDAVASAKSDAANKREVLHALGMAATDLRG
                     KFGEELASLQKFDLPLERATTASMEALAAFTEGRRLVREKGAIEAVPALKKALDLDPK
                     FALAHSNLAVAYYNLNQNVLAADHIRQAFELADRQTVRDRLHITTLYYDLATGDVQKA
                     IGSYKEWVRLYPRDDIAKGNLSSEYFLIGDYEQAANFAQQALRLDPGSAAWYENLATA
                     YIALQRLEEAQNILDLAFARKLDDPSMHADLYALAFLHGEPAGMEREMAWSAGKPGGE
                     DTMLALQADTEAYAGHVRKARELSRRAVEVAQNSQLNEPAAIWEGIAALREAVYGNLE
                     EARKGAEKVLEIAPNSRDAQILAILVLARVGDVRRAHTMLDDLAAANVSNTVVQSAWL
                     PTIRAQAEMVGQKPAQALELLDAVKLYERGQLIGNLSYSCMIPVYLRAESYLGAKLGP
                     QAFAEFQKLLDGRGVVGNCWSGALAHLGQGRAQALSGSTNAARTSYKEFFALWKNADP
                     DIPILKSARAEFAKLK"
     gene            2706..>3312
                     /locus_tag="VGR03_10535"
     CDS             2706..>3312
                     /locus_tag="VGR03_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994758.1"
                     /translation="MQVEEVHFNHDPSSISSDAITIRKNQAGNPIQAPEWKRGLTPQP
                     AAYAAAPLGANVTIKARFSGGQPDSALKIRAVDAWVPPATPMGCLGWLIWIISNLIRA
                     LFGNVLGDTDAQVVSFDGAGLSGLITFTLVNHKLKSSGVGIRTADWKWQYHRHYKWHT
                     FDTTQHKIYLVLDVPNGPWNQNAGNNTQLPWTDALDKACLWA"
BASE COUNT          662 a         1031 c          940 g          679 t
ORIGIN      
        1 cacgcattca ccatctgtgc tgggcgacgc catcaccatg atgggctcga tcggtttcgc
       61 catgtacgtt gtgcttggca aacgtctggc gggtcgctac gaccccttga ccatgacggc
      121 attcagccac tatgcgggag cgctaattgt gctacctgtc gcgatctatc gagctatcac
      181 gctcggctcc gcggagcact ggcgcgccat tgcctggacc ggctgggccg ccatgtttta
      241 catggcgatc ttcagctccg cagtcgccta cgtcttttat ttctggttgc tgcgctatct
      301 ggaagcctcg caattgagcg ccttcactta tgtctgccgg tcgtcgcaac catcatggga
      361 attctatggc tcggcgaaaa gggctcctgg gggcaggtgc tcggaggggt cctcgcgctc
      421 ggtggcgtct attggattga atctggccgg gcatctgtcg taaaagttat tgccgctccg
      481 taaactccgc ttgccaccgt cgttgacgag gtttctcctc aacacctact attattccaa
      541 atcagccctg aactcccgtt gacattccgg cagcattcat acgcggaagc aggctcatgg
      601 agcagccgca agacgcggca gctaactttc ggtaagagag gtctccgttc atgagggcca
      661 agctcgccat cgccggactg ctgctcgccc ttgtggccgc tggattttat tggtattcgc
      721 agcgcggcat gaaactgtcc gaaacggata ccattctgat cggggatttt gcgaacgccg
      781 ccggcgatcc cgtttttgat ggcacgctcc gagaagcgct ggccattagc ctggcccagt
      841 caccgtcgct gaacctggtt tccgtggaaa aagtcggcga agcgctacgg tcccttggtc
      901 gccccgtcga cgcccccatc acgcgggacc tcgctcccaa gctatgccag cgcctcggtg
      961 cgacggtcta cctcaccgga agcatcgaca aagatggcag tggttactcc gtgaggctgg
     1021 atgctgcgcg gtgctcgacc gacgatgccg tcgccagcgc gaagagcgat gccgccaaca
     1081 aacgcgaggt tctgcacgct ctcgggatgg ccgccaccga tcttcgcgga aagttcggtg
     1141 aagagcttgc ctctctgcaa aaattcgatt tgcccctcga gcgggccacc accgcatcca
     1201 tggaggccct cgcggctttc accgaaggac gccggttggt gcgtgagaaa ggcgccatcg
     1261 aggcggtgcc cgctctcaag aaagctctcg atctggaccc caaatttgct ctggcccact
     1321 ccaatctcgc ggtcgcctac tacaacctca atcagaacgt cctggccgcg gatcacattc
     1381 gccaggcttt cgaattggcg gatcgccaga ccgtgcgcga ccgcttgcac atcaccacgc
     1441 tgtactacga cttggctacg ggcgacgttc agaaggcgat cgggagctac aaggaatggg
     1501 tccgcctcta tccccgcgac gacatcgcta aaggcaatct gtcctcggaa tactttctga
     1561 tcggagacta cgagcaagcc gccaatttcg cccaacaagc gctccgcctc gatcccggta
     1621 gcgctgcctg gtacgagaat ctcgcgaccg cttacatcgc gttgcagcgt ctggaagaag
     1681 ctcagaacat tttggatctg gccttcgccc gcaaactcga tgatccctcc atgcacgctg
     1741 atctttatgc gctggccttt ttgcacggcg agcccgcagg catggaacgc gagatggcct
     1801 ggtccgccgg caaaccgggc ggtgaagata ccatgctggc tctacaggcc gacacggaag
     1861 cgtacgcggg ccacgtgcgg aaggcgcgcg aactctcccg ccgggccgtc gaggtcgcgc
     1921 agaactcgca gctcaacgaa cctgcagcca tctgggaggg aattgctgcc ttgcgggaag
     1981 cggtatacgg caatctcgag gaagctcgca agggcgcgga aaaggtactc gagatcgctc
     2041 cgaatagccg cgacgctcag atcctcgcca tcctcgttct ggcccgcgtc ggtgatgtgc
     2101 gtcgcgctca caccatgctc gacgatttgg ctgcagccaa cgtctccaat accgtggtgc
     2161 aatccgcctg gttgccgacg attcgggcgc aggccgaaat ggtcggccag aagccagcgc
     2221 aggctctaga attactcgat gctgtaaagc tctacgagcg cgggcagctc atcgggaatt
     2281 tgagctattc gtgcatgatc ccggtctatc tccgtgcgga gtcgtacctg ggagcgaagc
     2341 taggaccgca agccttcgcc gaattccaaa agctgctcga tggccgggga gtggtgggca
     2401 actgctggtc cggcgcgctc gcccacttgg ggcagggccg tgcacaggcg ttgtcaggtt
     2461 ccacaaacgc ggcgcggact tcctacaagg aattctttgc gctctggaaa aacgcggacc
     2521 ccgacatccc cattctcaaa tccgcacgcg ccgaattcgc caagctgaag taggtatcgg
     2581 ctcgttccgc gggcgtgctc aaaatcttgc ccagggtcaa caccaatatt cttcagcccc
     2641 gatttgaaac gaatcgtcgc ataccttcac tatagttaaa acaaccttcc gaaagcggag
     2701 gctcgatgca agtcgaagaa gttcatttca accacgaccc gagctcaatc tcctccgacg
     2761 cgatcaccat tcggaagaat caggctggca atcctatcca ggcccccgaa tggaagcgcg
     2821 gcttgacacc gcaacccgcc gcctatgccg ccgctcctct gggtgcgaat gtaacgatca
     2881 aggcgcgttt ctccggcggt cagccggata gtgccctcaa gattcgcgcg gttgatgcct
     2941 gggtgcctcc cgcgacaccc atggggtgcc tcggatggct gatttggatc atctccaatc
     3001 tgattcgcgc gctgttcggc aacgtgctgg gcgatacgga tgcgcaggtg gtgtccttcg
     3061 acggcgcggg cctttccggc ctgatcacct ttacgctcgt aaatcacaag ttgaaatcct
     3121 caggtgttgg cattcgaacc gcggattgga agtggcaata ccatcgccac tataagtggc
     3181 acacctttga cacgacgcag cacaaaatct atctggtact cgatgtgccc aacgggccct
     3241 ggaaccaaaa tgcgggcaac aacacgcagt tgccttggac ggacgcgctc gacaaagcct
     3301 gcctatgggc gc
//
LOCUS       DASYVO010000310         6526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_134986, whole genome shotgun sequence.
ACCESSION   DASYVO010000310 DASYVO010000000
VERSION     DASYVO010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134986"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VGR03_10540"
     CDS             complement(<1..317)
                     /locus_tag="VGR03_10540"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEV1994759.1"
                     /translation="MPPMRAVLKYSPKKLRVRRLLRSELPPDTVRLARFLLGKVVVHD
                     LPAGRLSGRIVETEAYPPGDPAGHHFRGPTPRIRSMYLPPGHAYIFFNYGAHFMLNVV
                     SEPG"
     gene            complement(390..761)
                     /locus_tag="VGR03_10545"
     CDS             complement(390..761)
                     /locus_tag="VGR03_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994760.1"
                     /translation="MTLLRLHKTLLNMERRDYEREHGYVNTGELFRLVIDHAQFAWLH
                     NISEFVVRIDETLAAKEPVTPEYTSGAISLARKMFVPTQSGDAFQKKYFDAIQRDPAV
                     VIEHAELARLFNNEPPDTSAP"
     gene            1081..1338
                     /locus_tag="VGR03_10550"
     CDS             1081..1338
                     /locus_tag="VGR03_10550"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEV1994761.1"
                     /translation="MEKNVSAADANRKFSELLRGVRKGRSYVVTSHGEPVAKLVPAAQ
                     DARIAAGARSALLARLKSQPVVKGAKTRRRWTRDELYEDGK"
     gene            1335..1778
                     /locus_tag="VGR03_10555"
     CDS             1335..1778
                     /locus_tag="VGR03_10555"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEV1994762.1"
                     /translation="MRVALDTNILAYAEGVNGAARRVNALELIQRLPAESALLPVQTL
                     GELFQLLVRKVNRTPAQARAAILGWRDAFPLIETSETVIVGAAELAATHRFSFWDGVV
                     LASAAEGGCRLLLSEDLQEGFTWNSVTVTNPFGVSPHPLLTDLLQ"
     gene            complement(1859..2788)
                     /locus_tag="VGR03_10560"
     CDS             complement(1859..2788)
                     /locus_tag="VGR03_10560"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEV1994763.1"
                     /translation="MRSQTLLAKSALLFSACAILSLSSFAQDATWQKNNAAWREEHVA
                     DLLKPDGWLSLVGLEWLQPGDTSVGSAADNKIHLAVGPEHLAILHLEDETVTLNPPAR
                     GFPSDFLVAGAPAKSQTLRAEANNDKVSLHLTIGTLNMYVIRREARFALRIKDSRSPA
                     IVGFHGLKWYAPDPAYRVTAKWIPYSPFKTITLATLVGTSYDQPVPGAAEFTLAGKIF
                     RIEPVLEDPAVAKLFFILRDTTSISATYGACRFLYTGFPTNGLDKPGELVLDFNHLEN
                     PPCAYTPYSTCPLPPLGNRLPIPLPVGEQRYHE"
     gene            3055..4245
                     /locus_tag="VGR03_10565"
     CDS             3055..4245
                     /locus_tag="VGR03_10565"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEV1994764.1"
                     /translation="MIVGVPRESFPGERRVALVPAAIPNLIKAGLEVVVEAGAGAGAG
                     YPDADYAAKGARIAADRAELFRAADIVVQILCYGSNDKTGKADVPLFRSGQALIGFLR
                     PLGSIETLQEIASKGVTSFSVELMPRTTRAQSMDVLSSMATICGYKAVVLAADTSPRI
                     FPMLTTAAGTITPARVLVIGAGVAGLQAIATARRLGAVASAYDLRPAAKEQVQSLGGR
                     FVELPIEAKDAEDARGYARPQDETFYKRQRELLGKVVAESDVVISAAVIPGKKPPILV
                     TKEMVASMAPGSVIVDLAGERGGNCELTRPGEIVVEHGVTIIGWFNLASTVPYHASQM
                     YARNVSAFLLHLVKDGKLQLNMDDEIIRETMLTRGGKVVNARVREFFSLPALPAESKE
                     VAKS"
     gene            4242..4547
                     /locus_tag="VGR03_10570"
     CDS             4242..4547
                     /locus_tag="VGR03_10570"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEV1994765.1"
                     /translation="MKLDLLTSLYVFMLAAFIGFEVIRRVSPLLHTPLMSLTNALDAI
                     AVVGAIILAGEYHAHGAMLAKILGTIAIVAATSNIVGGFLITDRMLRMFKASGPKKP"
     gene            4544..5938
                     /locus_tag="VGR03_10575"
     CDS             4544..5938
                     /locus_tag="VGR03_10575"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEV1994766.1"
                     /translation="MTNLPGLETIIEITYLIATALFILALKWLSSPKTARRGVFAGEL
                     GMLLAIAGTLLHHGIVEYKWIVIALVLGTIIGVPLGEVAMTAVPQRTALSHAFGALCV
                     TLVGTAEFYLRTPDVPRFMMGVLSLEVILGSLTFTGSLMAAGKLQEFLPQRPITYKGQ
                     NIVNLGLLGVAIAVAAMLVAHPEKTQLFPLIVGIPLLFGVMMIVPIGGADMPTVISLL
                     NSYAGLSAAAMGFVLDSKLLIIAGALDGASGLILSVNMSKAMNRSFSNVLFGAFGQQQ
                     TAVAGKQEARPVRSATPEEAAGILAAANKVVIVPGYGMAVAQAQHKVRELYDALTKRG
                     VDVKFGIHPVAGRMPGHMNVLLAEADIPYDKLLDMDEINGDFPQTDVALVIGANDVTN
                     PAARTDASSPIYGMPVLDVDKARTVMVIKRSMAAGFAGIDNPLYYLDKTLMLFGDAKS
                     FVGSIVRELGGGHE"
     gene            6162..>6526
                     /locus_tag="VGR03_10580"
     CDS             6162..>6526
                     /locus_tag="VGR03_10580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEV1994767.1"
                     /translation="MNGNDSLRSGSRRAFLQLSAAATAAMAFRIATEASLAAEERNVF
                     HPGAVVIDANENPLGPCDAARKAIVDMAPQGGRYSYWLKEEFVKTFTEMEGLKPEYLR
                     VFPGSSEPLHFSVLAFTSPA"
BASE COUNT         1365 a         1720 c         2104 g         1337 t
ORIGIN      
        1 ccaggctcgc tcacgacatt gagcatgaag tgagcgccgt agttgaaaaa aatgtaggcg
       61 tggccggggg ggagatacat cgaacgaatg cgcggagtag ggccgcggaa gtgatgtccg
      121 gcggggtcgc cgggcggata ggcttctgtt tccacgatgc ggccgctgag gcggcccgca
      181 gggagatcgt ggacgacgac ctttccgagg aggaagcgcg ccagcctgac agtgtcaggt
      241 ggaagttcgg agcgcagcag gcgccggacc cttaatttct tcggcgaata tttcagaacg
      301 gcgcgcattg ggggcatggg atctcgagtc ggtgaaaaag gaaccgctga aggttgtcgc
      361 cggaaagtct atcaggaaaa tcacaaaaac tacggcgcag aggtgtcggg aggctcgttg
      421 ttgaagagac gggcgagctc ggcgtgctcg atgacgacag cggggtcgcg ctggatggcg
      481 tcgaagtatt tcttctggaa ggcgtcgccg gattgcgtag gaacaaacat tttcctggcg
      541 agagagatgg cgccgctggt gtattccggg gtgacgggct ccttggcggc cagagtctca
      601 tcgatgcgga cgacgaattc ggaaatgttg tggagccagg caaactgcgc gtggtcgatg
      661 accaggcgga aaagttcgcc ggtgttgacg tagccgtgct cgcgctcgta gtcgcggcgt
      721 tccatgttga gaagagtttt gtggaggcga agaagggtca agcggaggga gagaagcttc
      781 tcgcgggcgg gggaatcgtt cttcgcggga tataaatttg agcttggaga atccattttc
      841 gagtgacacg tctattctcc gatagtcggc gtgcaaattc aaagtgaatg cgtgaagggc
      901 aattccttaa cggagagttc gcggaggtca cagagaagag cggagacaat gcaaaagcga
      961 aaatggaggt aggaaaggtt gcatctcaaa actgaaaact attcacctgc ttgatcgaaa
     1021 tcacttgtat ggcgcttgcg attggagcat agtaggtaat atcactactt tggagacggc
     1081 atggaaaaga atgtatcggc ggcggacgcg aaccgaaaat tttccgaact cctgcgaggg
     1141 gtgcgcaagg ggcggagcta cgttgtcaca tcgcatggcg agcccgtggc gaaattggtg
     1201 cccgccgccc aggatgcgcg catcgcggca ggggcacggt ctgcactcct cgctcgcctg
     1261 aagtcgcagc ccgtcgtaaa aggcgccaag acgcgccggc gctggactcg cgatgagctt
     1321 tacgaggacg gcaagtgagg gtagcgctgg acaccaatat tcttgcgtac gcggaaggtg
     1381 tcaacggcgc agcaagaaga gtaaacgcgc tggagttgat ccagcggctg cctgccgagt
     1441 ctgctttgct cccggtgcaa acgcttggcg agctgttcca gcttctggtg cggaaggtga
     1501 accgcacacc tgcgcaagcc cgcgccgcga ttctgggttg gcgtgacgcg ttcccactga
     1561 ttgagacttc cgaaaccgtg atcgtgggtg ctgccgagct tgctgccacg caccgtttca
     1621 gtttttggga cggcgtcgtc ctcgcctccg ccgccgaggg cggatgtcgg ctgctcctct
     1681 cggaagacct gcaagaaggc ttcacttgga acagcgtaac cgtaaccaat cccttcgggg
     1741 tgtcgcctca tccactgctc acagatttac tgcaatgagc tgaatagcgc gtgggccggc
     1801 atgccggttc ccgcctgagc tcaaaaatgg aaatccgaga taattgagaa taggacaatc
     1861 actcgtgata gcgctgttcg ccgacgggta gcgggatggg aagccggttg cccaggggcg
     1921 ggagcgggca ggtcgagtaa ggagtatagg cacatggggg attttccagg tgattgaaat
     1981 cgagcacgag ctcgccgggt ttatcgaggc cgttggtggg gaaaccggta tagagaaatc
     2041 ggcaggcacc gtaagtggcg gagatgctgg tcgtgtcgcg aagaatgaag aaaagcttgg
     2101 cgacggcggg atcttcgagg acaggctcaa tccggaatat ttttcccgcg agcgtgaatt
     2161 ccgcggcacc gggaacgggc tgatcgtagc tggtgccgac gagagtggcg agtgtgatgg
     2221 tcttgaacgg agaataggga atccatttgg cggtcacacg ataagcgggg tcgggcgcgt
     2281 accacttgag tccgtggaaa ccaacgatgg cgggagaacg agaatccttg atacgaagag
     2341 cgaagcgcgc ctcgcggcgg atcacataca tgttgagagt gccgatggtg agatgaaggg
     2401 aaactttatc gttgttcgct tcggctcgca gggtttgcga ttttgcggga gccccggcta
     2461 cgaggaagtc agaagggaat cctcgcgcgg gaggattcag tgtgacggtt tcgtcttcga
     2521 gatgaaggat ggcgagatgc tcgggtccaa cggcgagatg gattttgttg tcggcagccg
     2581 agccgacgga agtgtcgccg ggctggagcc attcgaggcc gaccagagaa agccagccgt
     2641 ccggcttgag gaggtccgct acgtgttctt cgcgccaggc agcattgttt ttttgccatg
     2701 tggcatcttg tgcgaacgaa gagagcgaaa gaatcgcgca agcggagaag agcaaggctg
     2761 atttggcgag aagggtctga gagcgcatgg aaaagtatag tcccagatgt aatcgagaca
     2821 ggctggcaaa aaattctttt agcaaagcaa ataggacaac cccaaaaaag tgatggtata
     2881 gaaacaaaca acaatcttgc gagggcactt acgaaaccgg ctccgaggag tgtatgcttt
     2941 ggggcgtctg gcccgctgga gggttggcct cgcgttgaac tttgtcgaac gaaacctctc
     3001 tatcgtttgt cagttccaac cgcagttctg gtttttagca acggaggaag cttcatgatt
     3061 gtaggtgtgc cccgagagag ctttcccgga gagcggcgcg tggcgctggt gcccgcagcc
     3121 attccgaatc taatcaaagc gggcctggaa gtggtcgtgg aggcgggggc cggagccggg
     3181 gcaggctatc cggatgccga ctacgccgcg aagggcgcaa ggattgccgc tgatcgcgcc
     3241 gaattgtttc gcgccgccga tattgtggtg caaattctct gctatgggtc caacgacaag
     3301 accggcaaag ccgatgttcc ccttttccgc agcggccagg cgctcattgg attcttgagg
     3361 ccgctcggct ccatcgaaac ccttcaggaa atcgcatcca agggagtgac ttcgttttcc
     3421 gtggaattga tgccgcgaac gacacgcgcc cagagcatgg acgtgctttc ctcgatggcc
     3481 acgatttgcg gctacaaggc cgtcgtgctt gccgcggata cttcgccgcg cattttcccc
     3541 atgctcacga cggccgcggg aacgattact ccggcccgcg ttcttgtgat tggcgcgggg
     3601 gtggcggggt tgcaggcgat tgccaccgcg cgccgccttg gcgcggttgc ttctgcctac
     3661 gatcttcggc ctgccgccaa agaacaagtg cagagcctcg gcggacgttt tgtcgagctc
     3721 cccatcgagg ccaaagacgc cgaagacgcg cgcgggtacg cacggccgca ggatgaaact
     3781 ttctacaagc gccagcggga actgttggga aaggtcgtag cagaaagcga cgtggtgatc
     3841 agcgcggctg tcattccagg aaagaagcct ccgattctgg tgaccaagga gatggtcgcg
     3901 agcatggcgc cagggtcggt gatcgtcgat ctggccggcg agcgcggtgg caattgcgag
     3961 cttactcgtc cgggagaaat cgtcgtggag catggagtga ccatcatcgg gtggttcaat
     4021 ttggccagca ccgtgccgta tcacgccagt cagatgtacg cacgaaacgt gagcgccttc
     4081 ctgctgcatc tcgtgaaaga cggaaagctg caactgaaca tggatgatga aatcatccgc
     4141 gagacgatgt tgacgcgcgg cgggaaagtg gtcaacgcac gcgtgcgaga gtttttttcg
     4201 ctcccggccc tgccggctga atcgaaggag gtagcgaagt catgaagctg gatctgctta
     4261 ccagcctcta cgttttcatg ctggccgcat ttatcggatt tgaagtcata cggcgcgttt
     4321 cccctcttct gcacacaccc ttgatgtcat tgacgaacgc gctcgatgcc atcgccgtgg
     4381 tcggagccat cattcttgcg ggtgagtatc atgcgcacgg tgcgatgctc gcgaagatcc
     4441 tgggaacgat cgccatcgtc gcggcgacga gcaacatcgt cggtggattc ctgatcaccg
     4501 accgaatgct gcgaatgttc aaagccagcg ggccgaagaa accatgacca acctgcccgg
     4561 cttggaaacc atcatcgaaa ttacctatct gattgccacc gcgcttttca ttcttgcgct
     4621 caagtggctg agttccccga agactgcgcg ccgcggcgtc ttcgccggcg aactgggcat
     4681 gctgctggcg atcgccggaa cgttactcca ccacggcatc gtcgaataca aatggatcgt
     4741 gatcgccctg gtgctgggaa cgattatcgg tgtcccgctc ggcgaagtgg cgatgaccgc
     4801 agtgccgcag cggaccgccc tgagccacgc gtttggcgcg ctgtgcgtga cgctggtcgg
     4861 tacggcggaa ttttatttgc gcacgccgga tgtgccgcgc ttcatgatgg gcgttctctc
     4921 tctggaggtc atcctcggat cgctgacgtt caccggcagt ttgatggccg cgggaaaatt
     4981 gcaggaattt ttgccgcagc ggcccatcac ttataaagga cagaacatcg tcaatctcgg
     5041 actcctgggt gtggcgatcg ctgtcgcggc tatgctggta gcgcatccgg aaaagacgca
     5101 gctcttcccg ctgatcgtcg gcattcctct tctgttcggc gtgatgatga tcgttcccat
     5161 cggcggcgcg gatatgccca cggtgatctc cctgctgaat tcctatgccg gactttctgc
     5221 ggcggcgatg ggtttcgtat tagacagcaa actattgatc attgcgggcg cgttggacgg
     5281 agcttccggt ctcattcttt ccgtgaacat gtcgaaagcg atgaaccggt cgttttccaa
     5341 cgtgctcttc ggtgcctttg gccagcagca aacggcggtc gcgggcaagc aggaggcccg
     5401 gccggtgcgc agtgcgacac cggaggaagc agcggggatc ctcgcagcgg cgaacaaggt
     5461 ggtcatcgtg ccggggtacg gaatggctgt ggcgcaggcg caacacaagg tgcgcgagtt
     5521 gtacgatgcg ctgaccaagc gcggcgtcga cgtgaagttt ggaattcatc ccgtcgccgg
     5581 ccgcatgccg gggcatatga atgttctgct cgcggaagcc gacattcctt acgacaagct
     5641 gctggacatg gacgaaatca acggagattt tccgcaaacc gacgtggccc tagtgattgg
     5701 cgcaaacgac gtcaccaatc ccgcggcacg cactgacgct tcgagcccga tttacggcat
     5761 gccggttctg gacgtggaca aagcccgcac cgtgatggtg atcaagcgca gcatggcggc
     5821 gggttttgca ggaattgaca acccgctcta ctacctcgac aagacgctca tgctcttcgg
     5881 cgacgcaaaa tccttcgtcg gctcgattgt gcgcgaactc gggggcggcc acgagtaact
     5941 cggcttgctt ttgatctgct gcaattcaag attccgattt tcctcatcat ttccttgagc
     6001 ttgcgattgc ggcggcaggg gtgcgacctt agccgagcgc cggaacagca atttgagtga
     6061 acgccatcca gttccatgtt gtatggcccc ggctttcgtg ctaatacaac tccatggcac
     6121 cacgcacccc cgcaacggtt tcgtctcgcc aggaggaccc catgaacggc aacgactcgc
     6181 ttcgcagtgg ttcgcgtcgg gcgtttctcc aattgtcggc tgcggcaacc gcggcgatgg
     6241 ctttccgcat cgccaccgaa gcatcccttg ccgcggaaga gcgcaatgtt tttcatcccg
     6301 gagcggtggt gatcgacgcg aacgaaaacc cgcttgggcc gtgcgacgcc gcgcggaagg
     6361 ccatcgtgga tatggcgccg cagggcggcc gctattccta ttggctgaag gaggaattcg
     6421 tcaaaacatt cacggagatg gagggcctga aaccggaata cctccgcgtc tttcccggct
     6481 cgagcgaacc gctgcatttc tcagtgctgg cgttcacttc accggc
//
LOCUS       DASYVO010000311         6354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_248053, whole genome shotgun sequence.
ACCESSION   DASYVO010000311 DASYVO010000000
VERSION     DASYVO010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248053"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VGR03_10585"
     CDS             complement(<1..247)
                     /locus_tag="VGR03_10585"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1994768.1"
                     /translation="MKLRWTPRALHHLNAAWDYVAKDNVAAADRLVQSILAAVESLQP
                     HPELGRKGRADATRELVIPGTPYIVVYRLRPAQIQVLA"
     gene            complement(244..483)
                     /locus_tag="VGR03_10590"
     CDS             complement(244..483)
                     /locus_tag="VGR03_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994769.1"
                     /translation="MSKQTVSFRLDARKVKALDTIAKAVQRDRTFLLDEAVQFYIHLH
                     EWQLKQIQESIVEADAGRLLDHAQVKNIVRKWRRR"
     gene            complement(826..1695)
                     /locus_tag="VGR03_10595"
     CDS             complement(826..1695)
                     /locus_tag="VGR03_10595"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV1994770.1"
                     /translation="MKRREFLHRTAGAAGAAWLHSSAFARRAFALPALSRKFSAADTV
                     TLGKTGIQTSRLAMGTGTVGSGHHSHQTALGVKGLSDLLLNGYDHGLRFFDAADSYGS
                     HPHVAEALMHVPRDKVTVLTKTWARDPATARADLDRFRRELGTDHIDVCLMHCLTEGD
                     WTDRYRGVMDVLSEAKEKGIIRAHGCSCHSIEALRVAAKSPWVEVHLVRINPIGASMD
                     SDPGTVVSVIREMRAAGKGIVGMKILGQGDLRHRQDEALNFALGLDLLDAFTIGAESQ
                     SEQEDLIRRIAAA"
     gene            complement(1787..2815)
                     /locus_tag="VGR03_10600"
     CDS             complement(1787..2815)
                     /locus_tag="VGR03_10600"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEV1994771.1"
                     /translation="MNDSFDDISGAPQPAPHVERTAHRQAETPNRLPRYVSYLFLLAA
                     LSTVAGYQVARLESSGNRWFGRTQLLVPASIQIPALVPASNPGATNTDVETISDLAPQ
                     QQAERLLELAIHRPDQSLGLIHKNLDSWRGHLEDTDQLFHLVLAALDSNDPRVRVAAV
                     EIDLVANNLRKSPQSLAKLLNQIHNDPGGRYLALWRLGALGNRGVEPATVLTTLLRYS
                     RDPNQQTRFWAVEGLAMLGTAESIDPLLSILAHDPAKQVRERAATNLARSGMLTGEQR
                     LTAVPQLLNLLDDDSLEPATQDLVYASLQTITGASFGRDPDAWRDWWAHHDRPEKQSH
                     VPAGLTQI"
     gene            3173..3364
                     /gene="rpmB"
                     /locus_tag="VGR03_10605"
     CDS             3173..3364
                     /gene="rpmB"
                     /locus_tag="VGR03_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV1994772.1"
                     /translation="MALKCEFCGKGPHFGNVISHANNTRRRRWNPNLKRVRAVVAGVR
                     KQVRVCTACIRAGKVKKAA"
     gene            3417..4526
                     /locus_tag="VGR03_10610"
     CDS             3417..4526
                     /locus_tag="VGR03_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEV1994773.1"
                     /translation="MKHSNGVMRAATWLPLAAMVLAAAAGCNKQVQHAPDVWAVVNGK
                     EIKRDEVDKYYRTRVNPEGQEPSQEESLSQKLNVLDELINNEILLEKAKKLNLEASDG
                     EVEDKFTELKSPYTEDEFQRRLKDQAMSVADLKLELRRQLSIQKLLNREVVAKIAITD
                     QDVTDFYNANKAQFNVAEPQYHIAQILVTPRKEQQIRNRKNDDATNEAEAQRKVKMLM
                     DRLNSGADFAQLAMDYSEDVNTAGTGGDLGYVPESALNQSDPALKKTVVGLKAGQVSP
                     PIQVQPKDGPRILILKLITRESPGLRGIAEPQVQQTIRDTLRNRKEQLLRLAYLAIAR
                     DGAQVTNFLAEQVIEAAGKLPDAAKTTVPQRSIGH"
     gene            complement(4599..5114)
                     /locus_tag="VGR03_10615"
     CDS             complement(4599..5114)
                     /locus_tag="VGR03_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEV1994774.1"
                     /translation="MRYEVQLASPSSGEKTRVIELERDASGWRVMLDGRPVAVDAVEI
                     TPNMLSILLDGQSFEISVTPTPDGQLKLQTGTQEFTAEVVDPRAWSGRRHGNVEAEGR
                     QQIVAPMPGKVVRLLVKAGDHVEAGQGLLVVEAMKMQNEIRSPKSGTVERVLAREGQP
                     VNAGEVLCVVA"
     gene            complement(5111..>6354)
                     /locus_tag="VGR03_10620"
     CDS             complement(5111..>6354)
                     /locus_tag="VGR03_10620"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="catalyzes the ATP-dependent carboxylation of a
                     covalently attached biotin and the transfer of the
                     carboxyl group to pyruvate forming oxaloacetate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV1994775.1"
                     /translation="AGLTFIGPPADAMESLGSKTAGRQLARRADVPTVPGTNDPIEKP
                     AEAQALAQRMGYPVLLKAVAGGGGKGMRLVYSDAEFDSAFRDASSEAMNAFGDDRLYL
                     EKYLERAHHVEIQIFADAHGRVVSLGERECSVQRRHQKVIEEAPSPIITPDLRKKMGD
                     AAVRLARAGGYVNAGTVEFLVDANLNFYFLEVNTRLQVEHPVTEQVTGLDLVKLQIAI
                     AAGHRLPFAWESITPRGHAMEVRLYAEDPDNNFFPSPGKILSRQVPGGPGIRLDDGVY
                     EGWTVPNDYDPLLSKLIAWGNSREETIARLRRALDEYTIIGIKTNVGLFRRILVEPDF
                     LRGEIHTRWLDELLGRPQSAASSAGENQPGSFASDAAAIAAAIWHANQSDRQASLSST
                     SADQPSRWKQEGRRAQLDRLP"
BASE COUNT         1360 a         1646 c         2168 g         1180 t
ORIGIN      
        1 tggcgaggac ttggatttgg gcaggccgga gacggtagac gacgatatag ggtgtgccgg
       61 ggatgacgag ctcgcgggtg gcgtcggcgc ggcctttgcg gccgagttca ggatggggct
      121 gcagggattc gactgcggcg aggatggatt ggaccaggcg gtcggcggcg gcaacattgt
      181 ctttcgcgac gtagtcccac gcggcgttga ggtgatggag cgcccggggc gtccagcgga
      241 gcttcaacgg cggcgccact ttctgacgat gtttttaacc tgagcgtgat cgaggagacg
      301 gccggcgtcc gcttccacaa tgctttcctg aatctgcttc aactgccatt catgaagatg
      361 gatgtagaac tgaacggctt cgtcgagaag gaaggtgcgg tcgcgctgaa cggctttggc
      421 gatggtgtcg agagccttca ctttgcgcgc gtccagacga aagctgacgg tttgcttgct
      481 cataagacga tataatacag aaaattattg tgtattacaa gggcgaacta gccagggatg
      541 gtggcgtaaa gccgcctggt tgggatgatc ccacgctgct cgttccgcca gaaacctttt
      601 caggggctaa gcttcgacag ccagagaaga gcagagagat ttctcactgc gcaggccgac
      661 cgcttcgcag gagcgaagtg ggaagaaaaa gtcggcctgc tccgttcgaa atgacgggtt
      721 ggaagtagca gcgtgccagg gggtgacgcg gagcatgacg acggcgtggg agagggcttg
      781 agcggtgaag gtggcggcgg atttgccaaa gctggagcga gagaactacg cggcggcgat
      841 gcggcggata aggtcttcct gctcggactg ggattcggcg ccgatggtga aggcgtcgag
      901 gaggtcgagc ccgagagcga aattgagggc ttcgtcctgg cggtggcgca gatctccctg
      961 accgagaatt ttcatgccga cgatgccttt gccggcagca cgcatttcgc gtatgacact
     1021 gacgacagta cccggatcgg aatccatgga ggccccaata gggttgattc gaacgagatg
     1081 gacttcgacc cagggagatt ttgctgcgac gcgtagcgct tcgatgctgt ggcaggagca
     1141 gccgtgagcg cgaatgatgc ccttttcttt ggcttcggag agaacgtcca tgacgccgcg
     1201 gtagcgatcg gtccagtcac cttcggtgag gcaatgcatg aggcagacgt cgatgtgatc
     1261 ggtgccgagc tcgcggcgga agcgatccag gtcggcacga gcggtggccg ggtcgcgcgc
     1321 ccaggtcttg gtaaggacgg tgactttgtc gcggggcacg tgcataaggg cttcggcgac
     1381 gtgcggatgg ctgccgtaag aatcggccgc gtcgaagaag cgcaggccgt ggtcatagcc
     1441 gttgaggagc aagtcggaga gacctttgac gccgagtgcg gtctgatgcg aatgatggcc
     1501 ggagccaacg gtgccggtac ccatggcgag gcggctggtc tggatgccgg tcttgccgag
     1561 ggtgaccgtg tccgcggcac tgaacttgcg cgagagcgcg gggagagcga aggcacgccg
     1621 ggcaaatgcg gacgagtgga gccaagcggc tccagcagcg ccagcggtgc gatggaggaa
     1681 ttcgcggcgt ttcataaacc ctccctgcag cggacgatcg aggcggtatg tctattatat
     1741 cgacgcgcgg gagagatgct tggttatccg agggatgagt tttagatcag atttgcgtga
     1801 ggcctgcggg aacatgcgac tgcttttcgg ggcggtcatg atgagcccac caatcgcgcc
     1861 aggcatcggg gtctctgccg aaagaggcgc cggtgatggt ttgcaacgac gcgtagacca
     1921 agtcctgcgt cgcgggctcg agggaatcgt cgtcgaggag attcaggagt tggggaacgg
     1981 cggtgaggcg ctgctcaccc gtaagcatgc cggaacgtgc gaggttggtg gcagcgcgtt
     2041 cgcgaacttg tttcgcggga tcatgggcca gaatggaaag aagcgggtcg atcgattccg
     2101 cggttccaag catggccaat ccctcgacgg cccagaagcg cgtttgctga ttcggatcgc
     2161 gagaatagcg aagaagtgtg gtcaataccg tggcgggctc gacgccacga ttaccgaggg
     2221 cgccgagtct ccagagcgcg agatagcgtc cgccgggatc gttatgaatt tgattgagaa
     2281 gttttgcgag actctgtggc gatttcctga ggttgttggc gacgagatcg atttccacgg
     2341 cggcgacgcg cacgcgagga tcgttggagt ccagagccgc aagcacaaga tggaagagct
     2401 gatcggtgtc ttccaggtgg ccacgccagg aatccagatt cttgtggatc aagcccagag
     2461 actggtcggg gcggtggatg gcaagttcga gaaggcgctc ggcctgctgt tggggcgcca
     2521 ggtcgctgat tgtttcaacg tcggtgttag tggcgccggg attggaagca ggcacgaggg
     2581 ccgggatctg gatggaggcg ggcaccagca gttgggtcct gccgaaccat ctgttcccag
     2641 aactttcaag gcgcgcgact tgataaccag cgacggtgga aagggcggcg agcaaaaaca
     2701 gatacgagac gtaacgaggc aagcgattcg gagtttccgc ttggcgatga gcagtccgct
     2761 ccacgtgtgg agcgggctga ggggccccgg agatgtcgtc gaacgaatcg ttcatgggtc
     2821 gcgcgagcgc gcagtcatag gcgcacgcta acgggcaagt taggtggagg caacgaggcc
     2881 aacaatggta cgggcggcca gcggaggttt catttgagaa caagccttgt agtttcaata
     2941 cgattggagg agttactggt acgcggcgcg cgtttgaatc ccgtaccaaa gaacaggggc
     3001 gtcgggggag gtggaagggc caagcgataa tgtacgttgt cccgcagaac cgtccggtat
     3061 gcgaaggaca cgctttgccg gaggtgaagg gtctgctaga ataaggagct tggttttgcg
     3121 acatttcgag caggaaaccc aagcccaaat aaaaactgaa gcaggaagaa aaatggcact
     3181 gaagtgtgag ttctgcggca aagggccgca ttttggaaat gtgatcagcc acgcgaataa
     3241 cacgcggcgg cgccggtgga atccgaacct gaagcgcgtg cgcgcggtgg tagcaggcgt
     3301 aagaaagcaa gtccgtgttt gcacggcgtg catccgcgcc ggcaaggtaa agaaagccgc
     3361 ctgagcctcc aaccttgaac ttgaccgttg cgaatctgcg ttccgtgggg catgcgatga
     3421 agcactcaaa cggagtgatg cgcgccgcaa catggctgcc tctggcggca atggtgttgg
     3481 ctgccgccgc gggctgcaac aagcaagtgc agcatgcgcc cgacgtgtgg gcggtagtga
     3541 acggaaaaga gatcaagcga gatgaagttg acaagtacta tcgaacgcgg gtaaacccgg
     3601 aggggcagga gccttcgcag gaagaatcgt tgtcgcagaa gctgaacgtt ctcgacgagc
     3661 tgatcaacaa cgaaattctg ctggaaaaag cgaagaagct gaacctcgag gccagcgacg
     3721 gcgaagtcga agacaagttc accgagctga agagcccgta caccgaagac gaatttcagc
     3781 ggcggctgaa agatcaagct atgtcggtgg cggatctgaa gctcgaattg cggcgccagc
     3841 tttcgatcca gaaattgctg aaccgtgaag tggtggcgaa gattgcgatc accgaccagg
     3901 acgtcacgga tttttacaac gcaaacaaag cgcagttcaa cgtggcggag ccgcagtacc
     3961 acattgcgca gatcctggtt acacccagga aagagcaaca gatccgcaac cggaagaacg
     4021 acgacgcgac caatgaagcc gaggcccagc gaaaggtgaa gatgctgatg gaccggctga
     4081 acagcggcgc ggacttcgca caattggcga tggattattc cgaagacgtg aacaccgcgg
     4141 gaacgggcgg cgacctgggc tacgttccgg aatcggctct gaaccaatcc gacccggcgc
     4201 tgaaaaagac cgtggtggga ttaaaggccg ggcaagtcag tccgccgatt caggttcaac
     4261 cgaaagatgg cccgcgcatc ctgatcttga agttgattac ccgcgaatcc cccgggctgc
     4321 gcggcatagc cgaaccccaa gtgcagcaaa ccatccgcga cacgctgcgc aaccgcaagg
     4381 aacaattgct gcgcctcgca tatctggcca tcgcgcggga tggggcgcaa gtgacgaact
     4441 tcctggctga gcaggtcatc gaggcggcgg gcaagttacc cgacgcggcg aaaacgaccg
     4501 tgccgcagcg atccatcggg cattaaagaa acttttccct ccggatttcc gggcgttcca
     4561 cttcagcctg gaataaaatt tctcaaatcg aataaagctt aagcgaccac acacaggact
     4621 tcgccggcat tgacaggctg gccttccctg gcgaggacgc gctcgacggt gccgctcttg
     4681 ggtgagcgga tttcgttctg catcttcatg gcttcaacaa ccagaaggcc ctggccggct
     4741 tcgacgtggt ctcctgcttt gacgagaaga cgcacaactt taccaggcat gggcgcaacg
     4801 atctgctggc ggccttcggc ttcgacattg ccgtggcggc ggccgctcca cgcgcgagga
     4861 tcgacgactt cagcggtgaa ttcttgcgtg ccggtctgga gcttcagttg gccgtccgga
     4921 gtcggagtta cgctgatttc gaaggattgg ccgtcaagta gaatggagag catgttggga
     4981 gtgatttcga ccgcgtcgac tgccaccggc ctgccatcca gcatcacgcg ccagccagag
     5041 gcatcgcgtt cgagttcaat gacgcgcgtt ttttctcccg aggagggaga cgcgagttgg
     5101 acttcgtatc tcatggcaaa cgatccaatt gcgcgcggcg cccttcctgc ttccagcgag
     5161 agggttggtc tgcggaagta gaggacagag aagcctgtct gtccgactgg ttcgcatgcc
     5221 aaattgcagc ggcgatggcg gcggcatcgg aagcgaagct ccctggctga ttttcgccag
     5281 cagaagacgc ggcggactgc gggcggccga gcagttcgtc cagccatctg gtgtgaatct
     5341 cgccccggag aaaatcgggt tcaaccagga tgcggcggaa aagtccgacg ttggtcttga
     5401 tgccaatgat agtgtactcg tcgagggcac ggcgaaggcg ggcaatggtt tcctcgcggc
     5461 tattgcccca ggcaatgagc ttgctgagaa gcggatcgta atcgttgggc acagtccagc
     5521 cctcgtagac gccgtcgtcc aggcgaatgc cggggccgcc gggaacctgg cgcgaaagaa
     5581 tttttcccgg tgaggggaag aaattattgt cgggatcttc ggcgtaaagc cgcacttcca
     5641 tggcgtggcc ccgcggcgtg atggattccc aggcgaaggg caggcggtgg ccggcggcaa
     5701 tggcaatctg gagcttcacc aggtcgaggc cggtaacctg ctcggtcacc ggatgctcaa
     5761 cctgcaaacg cgtgttcact tccaggaaat agaaattaag attggcgtca acgaggaatt
     5821 cgaccgtgcc ggcgttgacg tagccgccgg cgcgcgccag gcgcaccgcg gcgtcgccca
     5881 tcttcttgcg caggtcgggc gtgatgatgg gcgagggcgc ttcctcgatg actttttgat
     5941 ggcgccgctg gacggaacat tcccgctcac cgagagacac gacgcggccg tgggcgtcgg
     6001 cgaaaatctg gatttccacg tgatgagccc gctccagata tttttcgagg tagaggcggt
     6061 cgtcgccgaa agcgttcatg gcttcggaag atgcatcgcg gaatgcggaa tcgaattcgg
     6121 cgtcgctgta gaccaggcgc atgcccttgc cgccgccacc cgccacggcc ttcagaagaa
     6181 cgggatagcc catgcgttgc gccagggctt gcgcctcggc aggtttttca atggggtcgt
     6241 tcgtcccggg aacggtggga acatcggcgc ggcgggccag ctggcggccg gcagtcttgg
     6301 agccgagaga ttccatggcg tcggctggcg ggccgatgaa ggttagcccg gcgt
//
LOCUS       DASYVO010000312         4741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_62716, whole genome shotgun sequence.
ACCESSION   DASYVO010000312 DASYVO010000000
VERSION     DASYVO010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62716"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /gene="dacB"
                     /locus_tag="VGR03_10625"
     CDS             complement(<1..1290)
                     /gene="dacB"
                     /locus_tag="VGR03_10625"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEV1994776.1"
                     /translation="MRVLLRTGLRFRTEFFALTLLFAATAGFAQDPAPADLAGRIRQI
                     MSRPEFEHADFGIEFYAPDTDKVIYALNEQKLFTPASTTKLLTEGTLLASLGADYRFH
                     TKIFRTGPVDSRGKLKGDLILVASGDPNLSNRIRPGETLAFENEDHSYGGPAVAGDPL
                     AVIRQLARDVAAKGIRRIEGRVLVDASLFPDGAREGGTDVVISSIAVNDNLIDLFGKP
                     GAKIGDPLILETSPHTSYVKFVNHVVTTAAGSKPQLVPPGIQTNPDGSVTITLSGSLP
                     LDAPATPAAIPVPSPTRFAETVLREALVAAGIEVKSPAAAAAPDFAQYAKLYTPENQI
                     AEHVSPPLSEEIKVTLKVSQNLHAGMGPYLLGALVAKNAEDPLHSGFKIERDFLEAAK
                     LDLSGASQGDGAGGDWADLFSPHFMCRYLAYWTTRPDF"
     gene            complement(1391..1915)
                     /locus_tag="VGR03_10630"
     CDS             complement(1391..1915)
                     /locus_tag="VGR03_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEV1994777.1"
                     /translation="MRGFVLGIIVCLAAVAGAGWFYFTTGRAPVAVADPAMPFEKYLA
                     KKALASRIAKEKPKESPIPADEPNLLAGAATYKLNCAVCHGLPDGPRTNIAEGLFPRP
                     PQLLHGAGVTDDPAWETYWKAANGIRLSGMPSFKAKLSETQLWQVSQLLAHADKIPDS
                     VRKVLADTPAPAGR"
     gene            complement(2120..2512)
                     /locus_tag="VGR03_10635"
     CDS             complement(2120..2512)
                     /locus_tag="VGR03_10635"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEV1994778.1"
                     /translation="MHRNPARILATAFVLTLFAAASLPQMAGPGQAGTNNSTKKTRKA
                     SGSSKSNLVDINSASAAELEKVRGIDDALAKKIIDGRPYRTKRDLVTRKLIPQSTYDQ
                     IKDKIIAHRAGSQTAKKNTKDSGSTAPK"
     gene            2822..3430
                     /locus_tag="VGR03_10640"
     CDS             2822..3430
                     /locus_tag="VGR03_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994779.1"
                     /translation="MDESFHPTSFPAWVRWMSLVWLAVWIPSYAHYWGWLNFLHLCDI
                     GVFLTCLGFARSSTLLLSSQAVSSLVPDLLWCLDAGWRRFAGRQLIGGTEYMWDAGFP
                     LWVRLLSLFHVVFPVLLLLALRRTGYDRRGWILQCWIAAVLLAVSRFMGPEQNLNYTF
                     ADPVFHRSFGPAPIHLAVIFAGLAGIIYWPTHLLLARFFPSR"
     gene            complement(3662..4543)
                     /locus_tag="VGR03_10645"
     CDS             complement(3662..4543)
                     /locus_tag="VGR03_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994780.1"
                     /translation="MKTSVFLPVAACLFLSAASVAQEQAPPGPPKVLQIIREDVKPGK
                     GAAHAKVEAGWPRAFANAKSPTHYIAMVSITGPNEAWFVVGFDSLAAWEKDRQNNDKN
                     VALTTELDQLGEKDGELLTGIRSIVAVYREELSYRSTGINIGQMRYFYVTTFRVRPGH
                     DNDFVEANKIVRAAHEKADVPEHWAVFQVISGMPAGTYLSIQPLKSLAEVDAFPQTHG
                     KAYREATGDDGRKKLSELASAGSLSSETNIFAFSPKMSYVSKETASADPDFWKPKTVE
                     KAAAGANKKDAKPTGKP"
BASE COUNT          865 a         1347 c         1468 g         1061 t
ORIGIN      
        1 gaagtccggc cgcgtcgtcc agtaggcgag ataccggcac atgaagtgcg ggctgaagag
       61 atccgcccaa tcgcctccgg cgccatcgcc ctgcgacgcg ccggaaagat cgagtttcgc
      121 cgcctccaga aaatcgcgct cgattttgaa gccggaatgc aaaggatcct ctgcgttttt
      181 cgccacgagc gcgccgagaa ggtaaggccc cattcccgcg tgcaggttct ggctgacttt
      241 gagggttact tttatctcct cggacagcgg cggagaaaca tgctcggcaa tctgattttc
      301 cggtgtgtag agtttcgcgt actgcgcaaa atcaggggct gcggcggccg cggggctttt
      361 cacctcgatg cctgccgcga cgagcgcttc gcgcagcacg gtttcggcaa atcgcgtcgg
      421 ggaagggacg ggaattgccg cgggcgtggc cggtgcatcg agtggcaggc tgcccgaaag
      481 agttatggtg acgcttccat ccggattcgt ttggatcccc ggcggaacca attgcggctt
      541 cgaaccagcg gccgtggtca ctacgtggtt cacgaacttc acataagaag tgtgcgggga
      601 ggtctctaga atcaaagggt caccgatctt tgcgccgggt ttgccgaaca ggtcaatcaa
      661 attatcgttg acggcgatgg aggagatgac cacgtccgtg ccgccctcgc gtgctccgtc
      721 ggggaagagg ctggcgtcca cgagtacgcg gccctcgatg cgccggatgc ctttcgcggc
      781 aacgtctcgc gcaagctgcc ggatcaccgc gagcggatcg ccggcgactg cgggcccgcc
      841 gtaggagtgg tcttcgtttt cgaacgcgag agtctcgccc ggacgaatgc ggttggaaag
      901 gttcgggtct ccgctggcca cgagaatgag gtcgcctttc agtttgcccc tcgaatcgac
      961 cgggcccgtg cgaaaaatct ttgtgtggaa gcggtagtcg gcgcccaggc tggccaggag
     1021 cgtgccttcg gtcaatagtt tggtggttga agcgggcgta aagagttttt gctcattcaa
     1081 cgcgtaaatt actttatcgg tgtccggcgc atagaactcg atgccgaaat cggcgtgctc
     1141 gaattcgggc cggctcataa tctgccggat gcggccggcg aggtccgcgg gtgcggggtc
     1201 ttgcgcgaag ccggcggtcg ccgcaaagag cagcgtgagc gcgaagaatt cagtgcgaaa
     1261 tcgcaaaccg gtgcgaagca aaactctcac gattgcgttc ctccgggaat ttgttccagc
     1321 gcacagggcc attcgctgcg cccgccaggg atcggagcga gggcactttt tcgagaacaa
     1381 aaacggcgag ctaacggccc gccggcgctg gagtgtcggc aagaactttc cttacggagt
     1441 cggggatctt gtcggcgtgc gccagcaact ggctgacctg ccagagctga gtctccgaga
     1501 gctttgcttt gaaactgggc atgccgctca agcggatgcc gttcgcagct ttccagtagg
     1561 tttcccacgc cgggtcgtcg gtgacacccg ccccgtgaag caactgagga ggccgtggaa
     1621 agaggccttc ggcgatgttg gtcctgggcc catccggcag gccatggcac acggcgcaat
     1681 ttagtttgta ggtcgcggcg cctgccagaa gatttggctc atcggcaggg atgggagatt
     1741 ctttgggctt ctcttttgca atgcgtgacg ccagtgcttt tttcgcgagg tatttttcaa
     1801 acggcatcgc cggatcggcc actgcaaccg gggcgcggcc cgtggtgaag taaaaccacc
     1861 cggcgccggc gacggcggcg aggcagacaa taattcccag aacgaatccc ctcatgaaat
     1921 atcctcctca accaaaacac cgaaagcata caagacggag gagaggaatg ccactgcggt
     1981 cgttgcggcc agagacagag aaatgtttcg gcgggcacac cactagtgat tcggcgagat
     2041 cgctgcgagt ggcgcgactg cgctcgaaac gggaggacaa tccagacgtt ctttcagcgg
     2101 cgtcgaggag caggcgaatt cattttggtg cggtggagcc gctgtccttt gtgtttttct
     2161 tggcggtctg gctgccggcg cgatgggcga tgatcttgtc cttgatctga tcgtaggtgg
     2221 actggggaat gagtttcctc gttacgaggt cgcgcttggt gcggtagggc cggccgtcaa
     2281 tgatcttctt agctaaagcg tcatcgattc cccgcacctt ttcgagttcc gccgcgctgg
     2341 cggagttgat gtccacgaga ttgctcttgg aactgcccga ggccttcctg gttttcttcg
     2401 tggagttgtt cgtccccgcc tggcccgggc ccgccatttg cggcaacgag gcagcggcga
     2461 acaatgtcaa gacaaaagct gtagcgagaa ttcgagctgg atttcggtgc atgggttgtt
     2521 ctcctttatg ggcagagcat ggaaacgcca aagtgccggg agtaggactc ggttatttat
     2581 ctcataatat acttaagaag gaaagagttt cgtctacccg cagcaggctg ctgaaaaagc
     2641 gcctccggtc gtcattccaa ggagaacgct ggcggattcg tggcgaaggc gcgaagtttg
     2701 gctggccgga tttcgtttcc ggcaagggcc gcccgaaggc gtaaactctc agcctgtaga
     2761 tttcggtcgt gcgccgcgct ttgcgatgct gtattcgctc gggatgggct gaggataagc
     2821 catggatgag tctttccatc cgacctcgtt tcccgcctgg gttcgttgga tgagcctggt
     2881 gtggctggcc gtctggattc cgtcttacgc gcactactgg ggttggctca atttcctcca
     2941 cctttgcgat attggggtct ttttgacgtg cctcggcttt gcgcgcagca gtacgcttct
     3001 gctttcaagc caggcggtct cttcactcgt gcccgaccta ctatggtgtc tggacgcagg
     3061 ctggcggcgg ttcgccggca ggcagttgat cggcggtacg gaatacatgt gggacgcggg
     3121 gttcccgctc tgggtgcgac tgctttcgtt gttccatgtt gttttcccgg tgctgttgtt
     3181 attggcgctg cgccgcacag gctatgaccg ccgcggttgg atcctccagt gctggatcgc
     3241 agccgtcctc ctggcggtgt cgcgttttat gggcccggag cagaatctga actacacatt
     3301 tgccgacccc gtcttccatc gctccttcgg gccggcgcct atccatctag ctgtgatttt
     3361 cgccgggttg gcgggaatca tctattggcc cacgcatctc cttcttgctc ggttcttccc
     3421 atcacggtag cccgccgtct cgagaggctc aggacgtgga cgcagagcgc atgagaaaag
     3481 gccgggtcct gtcacccggc ctgcacttct cgaattgatt gcaggggttt cccaataagt
     3541 agaggcgtgg cctctgctgc cgcgggagcc gagactcaga aaaacgcaag agcatcccgc
     3601 cccgtaggga cgggatgcga gcgtcctcgg ctccgaatgg ttgcgtcccg acgcccgagc
     3661 cttatggttt ccctgtgggc ttggcgtctt tcttattggc acctgcagcg gctttctcca
     3721 ccgtcttcgg tttccagaag tctgggtctg ccgaggctgt ctcttttgag acgtagctca
     3781 tcttcggact aaaggcaaag atgttcgtct ccgagctcaa agagccggcg ctggccagct
     3841 cacttagctt cttccggcca tcgtctccgg tcgcctcccg gtaagccttt ccgtgcgtct
     3901 gaggaaacgc gtccacctcc gcaagcgatt tgagaggttg gatgctcagg taggtccccg
     3961 cgggcatccc ggatatgact tggaaaacgg cccagtgctc gggcacatcg gccttttcgt
     4021 gcgcggctcg gacgatcttg ttcgcctcga cgaaatcgtt gtcatggccc gggcgaacgc
     4081 ggaacgtcgt tacataaaag tagcgcatct gtccgatgtt gatcccagtg gaccgatagc
     4141 tcaactcttc ccgatagacg gccacaatgc tgcggattcc agtgagaagc tccccatctt
     4201 tctcaccaag ctgatcgagc tcggtagtca gcgcaacgtt cttgtcgtta ttctggcgat
     4261 ccttctccca ggctgccagc gaatcaaatc ccaccacaaa ccaagcttcg ttcgggccag
     4321 tgatggacac catcgcgata tagtgcgtcg gcgactttgc gttggcaaaa gcacggggcc
     4381 atcccgcttc cactttcgcg tgcgcagcgc ctttccctgg ctttacgtcc tcgcggatga
     4441 tttgaagcac tttcggcggg cccggaggcg cttgctcctg cgcgactgag gctgccgaaa
     4501 gaaacaaaca ggcggctacc ggtagaaaca cacttgtctt cacggtcggt tctctcattt
     4561 tgctgaaagt gagagcgcgc ggaagagagc aacccgcaag atacaggccc tgggttcagc
     4621 tagccacctc tcccccagag cgctctgcag tggtgcgccg caagtgtact gcgttcgctg
     4681 ttcaagtcaa cctattcgga aggggagaaa cttgtcgggc tcgcgctccg ctctgcgaag
     4741 g
//
LOCUS       DASYVO010000313         4846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_36752, whole genome shotgun sequence.
ACCESSION   DASYVO010000313 DASYVO010000000
VERSION     DASYVO010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36752"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2346
                     /gene="lptD"
                     /locus_tag="VGR03_10650"
     CDS             <1..2346
                     /gene="lptD"
                     /locus_tag="VGR03_10650"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEV1994781.1"
                     /translation="LFLAGLLFALAAATPGIASAQQTRFPSAKGGVANLEAKTQSRKG
                     DVTAADGDVDIHYADTRLRADHVEYNSKSYEATATGHVQLDYGNEHLEADEAHYNVST
                     GHGLFHNVRGTVKIERHPNPVLLISDNPLYFEARDVERFPGDVYLIHRAWITICDPQH
                     PKWQFYAPNARLRLDKTVVLINANFRLFRVPLIWLPYASAPAGRRVRASGVLIPDVGQ
                     SSRKGFILGDAVFLAPTPWMDATLGAQYMSRRGFLERGSFRAKPFENTSIEYTYFGVE
                     DRGLRNADGTRSPQGGEQQRLEIQSLLPSGWRFVTDFNHLSSLTFRLAFADSFGEATN
                     SEVRSAVFLSNNSRGFSMNFAGLSDKNFLTLPTTTTSSTGVVTPVPATSVTLRNLPEA
                     RLGSVEQAPWRSVPVYFGFDAFAGVVYRRDQNIQTPNFVDRYEFAPRVTVPLHLGYWL
                     GVTTSAAFRTTSYGDSLDSTGNISVKSIKRNTGEFTVELRPPTLERFFDRASLKKDKT
                     RRRYKHTIEPALTYRYVTGVNHFADFIRFDSDTTLTNTSEVEYGFTQRLFRKEGDGQP
                     QELISWRIVQKHYFDPTFGGAIVNGQRNVFQAFDSISPFAFAFGPRNWSPIVSDFKIT
                     PGGLYDLEQILQYDPQLQRLVTIGTLLKVKPYREFFATVSYFRLNANPAQSDLPVPPP
                     RQFIAFKQPDSNQIRGLVGYGSETRKGFNLITGVGYDFENKTLQNQIVQVSYNGGCCG
                     LAVEYRRINLGQVRAENQFRVAFIIANIGAFGNLRHQERIF"
     gene            2412..3833
                     /locus_tag="VGR03_10655"
     CDS             2412..3833
                     /locus_tag="VGR03_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV1994782.1"
                     /translation="MNDTDLAFASIEEIGKLFRKRKISPVELTKLMLARIEQLNPKLN
                     AYITVTAELALAQAKKAESELFAPRGRRGNRDRGPLHGVPISLKDNIYTAGIRTTAGS
                     KILRDFIPQHDAQVVVMLKEAGTVLLGKTNMHEFAYGVTSNNPHYGPVRNPWDLERIP
                     GGSSGGAAAAVASGLCYGSIGTDTGGSIRIPASLCGIVGLKPTWGRISCEGTVPLSPL
                     LDCTGPLARTVRDVAILTGAVYFRVGREPDLATPGSLRSDPRKFCLGIPRQLFFDALS
                     TDVRKAFDSALRDLCKAGMKTTDISIPLLDETEDAGNHIAWVEAILFHSRQGYFPGRA
                     AEYGEDVRSRIEMGTKVSALDYLQALEIQKQFVLQLHLALAESDADAIAFPSTAIEAP
                     LLNQETIRIGVHEYPARALLLRNNRPANLAGVPAVSVPCGFTPSRLPIGLQLMGGVSS
                     EPVLLRIAKIFEGTQPQFHRPPI"
     gene            complement(3830..>4846)
                     /locus_tag="VGR03_10660"
     CDS             complement(3830..>4846)
                     /locus_tag="VGR03_10660"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1994783.1"
                     /translation="AIVWRGEVSTAEREARAPGFEGTERAIAALIAREAAAAWAACGA
                     FEWVALGYLAASSALIAVFAQNLAHPVKLFGVQALVAAMILVLCFVEARVAERAERRG
                     ETVTSKFWHFWRHWYPHLFFLFCFEELGKLVHLVKPGWQDAKLIASDYWLTGAHPTVW
                     LEQFATPARNDFMQFAYLTYFTYLLVLGGVLYYRRDWHGYWSVMTYSAAGYAIGYGIA
                     IFFPIESPWFSMAGAWHGSLQGGPFTAAINFIEHFGRVRGAAFPSEHVAGSVAALWGA
                     WRHRRWLFWTIFPLVTCMCVSTVWGRYHYVVDVFGGMVTGTLGYVIGSWIMKRRGAVA
                     AEAR"
BASE COUNT         1001 a         1661 c         1199 g          985 t
ORIGIN      
        1 ctcttcctgg cagggcttct cttcgcgctt gcagccgcga cgccaggcat cgcctccgcc
       61 cagcaaaccc ggtttccttc cgccaaaggc ggagtcgcaa atctggaagc gaaaacgcag
      121 agccgcaaag gcgacgtcac cgcggctgac ggcgacgtgg acattcacta cgcggacact
      181 cgtcttcgcg ccgaccacgt cgaatacaac agcaagagct acgaagccac cgctaccggc
      241 cacgtgcagc tcgactacgg caacgagcac cttgaagccg acgaagctca ttacaacgtc
      301 agcaccggac acggcctctt tcacaacgtc cgcggcaccg tcaagatcga gcggcatccc
      361 aatcccgtac tcttgatttc cgacaatcca ctctacttcg aagcacgaga cgtcgaacgc
      421 ttcccgggag acgtctacct catccaccgc gcctggatca ccatctgcga cccccaacac
      481 cccaagtggc agttctatgc gcccaacgcg cggctccgtc tcgataaaac cgtcgtgctc
      541 atcaacgcca acttccggct tttccgcgtt cctctcatct ggctccctta cgcctccgcc
      601 cccgcgggcc gcagagtccg cgcgtccggt gtcctcattc ccgacgtcgg ccagagctcg
      661 cgcaagggct tcattctcgg tgatgctgtt tttctggctc ccactccgtg gatggacgcg
      721 accctcggcg cccagtacat gagccgccgc ggctttctcg aacgcgggtc attccgcgcc
      781 aagcccttcg agaacacttc gattgagtac acttattttg gcgtcgagga ccgcggcctc
      841 cgcaacgctg acggcacgcg cagtccccag ggtggcgagc agcagcgcct cgaaatccaa
      901 tcgctgctcc ccagcggctg gcgcttcgtg acggacttca accatctctc gtcgctgact
      961 tttcgtcttg ccttcgccga ctctttcggc gaagctacca actccgaagt gcgcagcgcc
     1021 gtcttcctct cgaacaattc ccggggcttc agcatgaatt tcgcgggcct cagcgacaaa
     1081 aactttctca cgcttcccac gaccacgacc tcgtccaccg gcgttgtcac tccagttccc
     1141 gccaccagtg tgacgcttcg caatctgccc gaagcgcgac ttgggtccgt cgagcaagcc
     1201 ccctggcgta gcgtccccgt ctatttcggc ttcgacgcgt ttgccggtgt cgtgtaccgc
     1261 cgcgatcaaa atattcaaac tccaaatttc gtcgatcgct acgaattcgc accgcgcgtt
     1321 acggttccac tccatctagg ttactggctg ggcgtaacca cttctgccgc tttccgcacg
     1381 acgagctacg gcgattcgct cgattccacc ggcaatatta gtgtcaagtc catcaaacgc
     1441 aatactggcg agttcaccgt cgaactccgc ccgcctacgc tcgaacgatt cttcgaccgc
     1501 gcttccttga agaaggacaa gactcggcgc cgctacaagc acaccatcga acccgctctc
     1561 acctaccgct acgtcaccgg cgtgaatcat tttgccgact ttatccgttt cgattccgac
     1621 accactctta ccaacaccag cgaagttgaa tacggcttta cacaaaggct ctttcggaag
     1681 gaaggtgacg gccagccgca agagctgatt tcctggcgca tcgtccagaa gcactatttt
     1741 gatccgacct ttggcggcgc catcgtcaac ggccagcgca acgtttttca ggcgttcgac
     1801 tccatctcgc ctttcgcttt cgctttcggt ccccgcaact ggtctcctat cgtcagcgat
     1861 ttcaagatca ctcccggcgg cctctacgat ctggagcaga ttcttcagta cgatccccag
     1921 ctccagagac tggtcaccat cggcacgctc ctgaaagtaa agccgtatcg ggaatttttc
     1981 gccacggttt cctacttccg cctgaatgcg aatcccgcac agagcgatct gcccgttcct
     2041 ccgcctcgcc aattcatagc tttcaaacag ccggattcga accagatccg cgggctcgtc
     2101 ggctacggaa gcgaaacccg caagggcttt aatctcatca ccggtgtcgg ctacgacttc
     2161 gagaacaaga cgctgcaaaa ccagatcgtt caggttagtt acaacggcgg ctgctgcggc
     2221 ctcgccgtcg aatatcgccg catcaacctc gggcaggtgc gcgcggaaaa tcagttccgc
     2281 gtcgcgttca tcatcgcgaa catcggcgcc ttcggaaacc tccgtcacca ggaaagaatc
     2341 ttctaaccgg gggcttgtgc agcaccgggc tttagcctgg acatcttcat ttgtgattta
     2401 atgtgacacc gatgaacgat accgatctgg cgttcgcatc catcgaggaa atcggaaaac
     2461 ttttccgcaa acgcaagatc tcgcccgtcg agctcaccaa gctcatgctc gcccgcatcg
     2521 agcaactgaa tcctaaactg aacgcttaca tcaccgtgac cgctgaactt gctctcgcgc
     2581 aggccaagaa agctgaatcg gaacttttcg caccccgcgg ccgcaggggg aatcgtgatc
     2641 gcggcccgct tcacggcgtg cccatttcac tgaaagacaa catctatacg gcgggcattc
     2701 gcaccacagc cggttccaag attctgagag atttcattcc acagcacgac gcccaagtcg
     2761 ttgtcatgct gaaagaagcc ggcaccgttc tccttggcaa aaccaacatg catgagttcg
     2821 cgtacggcgt aacttccaac aatccccact acggccccgt ccgcaatccc tgggatctcg
     2881 aacgcatccc cggcggctcg agtggtggag ccgccgccgc tgttgcatcc ggcctttgtt
     2941 acggaagcat cggcacagat accggcggct ccatccgcat tccggcttcc ctctgcggca
     3001 tcgtgggcct aaagccaact tggggccgca tctcttgtga aggcaccgtc ccactctcgc
     3061 ctttgctcga ttgcaccggc ccgctggcgc gtactgtccg cgacgtagct atcttgacgg
     3121 gcgcggttta ttttcgcgtc ggccgcgagc cagacctggc gacacccgga tctttacggt
     3181 ctgacccgcg gaagttttgt ttgggtattc cgcgtcagct ctttttcgat gcgctctcca
     3241 cagacgtgcg gaaagccttt gattctgccc tgcgcgatct ctgcaaggcg ggtatgaaga
     3301 ctaccgacat ctcgatcccc ctgctggatg aaaccgaaga cgcgggcaat catatcgcct
     3361 gggttgaggc gatacttttc cattcacggc agggatattt tccaggccgg gccgcggagt
     3421 acggcgaaga cgtgcgttcg cggattgaaa tgggcacaaa ggtttccgcg ctcgactatt
     3481 tgcaagcctt ggagattcag aaacagtttg tgctacaact tcatctggcc ctggcggagt
     3541 cggatgccga tgccatcgcc tttccctcga ccgccatcga ggccccgctc ttgaaccagg
     3601 aaactatccg tatcggggtg cacgagtatc cggctcgggc tttgctgctg cgcaacaacc
     3661 gtccggcaaa tctggccggt gtgcctgccg tttccgttcc ctgcggcttt acgccttctc
     3721 gattgccgat tggtttgcaa ctcatgggcg gcgtttcgag cgagcccgtt ttgcttcgca
     3781 tcgccaaaat cttcgagggc actcagccgc aatttcacag gccgccgatt tagcgcgctt
     3841 ccgctgcgac cgcgcctcgc cttttcatta tccaactgcc aatcacatac cccagcgtcc
     3901 ccgtcaccat tcctccaaac acatccacca cgtagtgata ccgtccccac actgtcgaaa
     3961 cgcacatgca cgtcaccagc ggaaatattg tccagaacag ccaccgccgg tgccgccaag
     4021 cgccccacaa cgccgcgacc gaccctgcaa catgctcgga aggaaatgcc gcgccgcgca
     4081 cgcgcccaaa atgctcgatg aaattgatcg cggctgtgaa cggcccgccc tgcagcgatc
     4141 catgccacgc accggccatc gaaaaccatg gactctcgat tggaaagaag atcgcgattc
     4201 cgtaaccgat tgcatagccc gcggccgaat aggtcatcac cgaccagtac ccgtgccaat
     4261 ctcgccgata atacaagacc ccgcccagca ccaagagata cgtgaagtag gtgaggtagg
     4321 caaactgcat gaaatcgttt cgcgccggcg ttgcaaactg ctccagccac accgtgggat
     4381 gcgcgccggt cagccagtaa tcggaagcga tcaacttcgc gtcctgccag ccgggcttca
     4441 ccagatgcac gagctttccc agttcctcaa aacaaaacag aaaaaacaga tgcggatacc
     4501 agtgccgcca gaagtgccag aactttgagg tgactgtctc gccccgccgc tccgcgcgct
     4561 ccgcaacccg ggcttctaca aagcacaaaa ccaagatcat cgccgcaacc aacgcttgca
     4621 caccgaacaa tttcactgga tgtgcaaggt tttgcgcaaa caccgcgatc agcgcgctcg
     4681 atgccgccaa gtatcccagc gccacccatt cgaatgctcc gcacgccgcc cacgccgccg
     4741 cagcttcccg cgcgatcagc gccgcaattg cgcgttccgt accttcgaag cctggcgcgc
     4801 gcgcctccct ctcggcagta ctcacctccc cgcgccagac gatcgc
//
LOCUS       DASYVO010000314         8378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_110232, whole genome shotgun sequence.
ACCESSION   DASYVO010000314 DASYVO010000000
VERSION     DASYVO010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8378
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110232"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VGR03_10665"
     CDS             <1..946
                     /locus_tag="VGR03_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1994784.1"
                     /translation="ETIIMYIGGYDDMVVAKTQIRGRIEADNANREKKIAQLQEFIAR
                     FSAGTRSAQATSRKKEVERLQTAELARSNIQRPYIKFDMKRNSGRHPLEIRSVEKSYE
                     DLKVIPRFSASVVRGEKIALMGRNGAGKTTLLKSLVRNAPGFIEDKDLQFPIDGGEVR
                     WGHEVAVGYFAQDHGESITKGMTAIEWLWQFDPAASQQELRGLMGQMLFSGEDALKPT
                     RALSGGESARLIFCRLMLQKPNFLVLDEPTNHLDLESINALNIALQRYDGTVLLVTHD
                     HDVIDEVATRIWHFQGGKVEDFKGPYEEYVAYAEQQAS"
     gene            complement(977..2140)
                     /locus_tag="VGR03_10670"
     CDS             complement(977..2140)
                     /locus_tag="VGR03_10670"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1994785.1"
                     /translation="MIRKFDAVVLGAGAAGLLCAIEAGERGRRVAVIEHADRVGKKIL
                     ISGGGRCNFTNIHCQPENFISANEHFAISALARYTPADFIALVEKYRIPYHEKTLGQL
                     FCDRSAQDIIDLLESECQAAGVQTFLQSRVREVQRTTEFVVCTESGEFQAPALVVATG
                     GLSIPKMGATPFGYQLAQQFGLKIVETKPALVPFVLTGGDRSRYCDLAGVSAEVVAKT
                     DQHAFREKMLITHRGLSGPAILQISSYWEKGQSIQIDLAPDQDITVAIRAAKVRNMAA
                     AQKAFQGILPKRFAERWLENHAPAAWNDDALTKWEEQVHDWGIKPADTEGYEKAEVTA
                     GGVDTAELSSKTMECLNVKGLFFIGEVVDVTGHLGGFNFQWAWASGAAAGRAL"
     gene            2236..4053
                     /locus_tag="VGR03_10675"
     CDS             2236..4053
                     /locus_tag="VGR03_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1994786.1"
                     /translation="MPPIISAQGLSKRYGVAPLFQNISFTVSEGDRIGVIGPNGSGKS
                     TLLEMFIGRVKPDGGDVAVRKGTRLSYVAQISEFAAGVTIRSVIESALERTAVPETER
                     ASRFAETLGRAGFTDLDAHAATLSGGWRKRLAIAEALVQAPGILLLDEPTNHLDLAGI
                     QWLESVLRNAAFACVLVSHDRYFLENVANEMVELNRAYEDGALRVKGNYSAFLEAEEL
                     YLHAQKKRQESLENRVHTELEWLRRGPKARATKAKARIDKAHEMIGELAEMNARSRTS
                     SAEIDFSATNRKTKQLINLEEVSYGVGSRTLFKEIHFNVTSGMRVGLVGPNGSGKTTL
                     LRLIRGDIKPESGKVRRAEGLRVVYFDQNRELDPDITLRRALAPDSDAVIYQERVIHV
                     AAWAERFLFSAENLNQRVGRLSGGERARVLIAQLMLQPADVLLLDEPTNDLDIPTLEI
                     LEESLLEYPGALVLVTHDRFMLDRVSTVVVGLDGFGAAERFADYSQWETWQRAQLAMA
                     SAKSADGNRRPRSVADEAPCTAAKKKLSYAEAREFAAIEGRIHEAELMLQARRAALED
                     RAISSDRMSLQNACSQLDEAQKSVDALYARWAELEQKQG"
     gene            complement(4142..4933)
                     /locus_tag="VGR03_10680"
     CDS             complement(4142..4933)
                     /locus_tag="VGR03_10680"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV1994787.1"
                     /translation="MKRITLISLLILCAAQVASAQDWARATLEKSPRHREWVTIKHDG
                     RSVETFVVYPESKGKTPVVLIIHEIFGMTDWVQDLADQVAAAGYIAVAPDLLSGMGPN
                     GGRSSDFAEGKTMEAISHLNPDQVTADLNAAADYAMKIPASNGKLLVGGFCWGGGQTF
                     RFATNRADLAAAFVFYGPPPDKDAMARIKAPIYGFYAGNDARIGALIPDAVAQMKAAG
                     KTFDIVTYEGAGHGFMRAGEAPDAKDADKKARADAWVRWKSLLGK"
     gene            5154..6593
                     /gene="proS"
                     /locus_tag="VGR03_10685"
     CDS             5154..6593
                     /gene="proS"
                     /locus_tag="VGR03_10685"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV1994788.1"
                     /translation="MAEEKLPTRAQDFSEWYNQLVLKAQLADYAPVRGCMIVRPYGWA
                     LWENIQQALDRRFKATGHQNVAFPMLIPRSFIDKEKHHVEGFSPELAVVTIGGGEELA
                     EPLVIRPTSETIIGHMWSKWIQSYRDLPVLMNQWNSVVRWELRTKLFLRTLEFYWQEG
                     HTAHATREEAEAETLQMLDIYADFAVNDAAIPVIPGKKSEAEKFAGAHTTYAIEAMMG
                     DGKALQSGTSHFLGQNFAQAFEVKYLDQNGQLQHCWTTSWGLSTRIIGAIIMVHGDDQ
                     GLVLPPKLAPYQVVIVPIFKTDEEKASVLDTARKLKAELLKANIRVTLDEREGQSPGW
                     KFNDWEMRGVPLRVELGPKDVAKQSAVLSRRDRPGREGKISASLAGLPTSIEGLLVEI
                     QQSLHDKALAFRKSNTHDAKTYDDLKKAVENGFAFSFWCGSADCEAKIKEETRATMRC
                     IPLEQEGGGGTCVYCAQPATARAIFARAY"
     gene            6741..7145
                     /locus_tag="VGR03_10690"
     CDS             6741..7145
                     /locus_tag="VGR03_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994789.1"
                     /translation="MHLEGIRDRRALAHCRSERGEGKLKAIIYLAILLLVIYSAVKIV
                     PIYVSNYQLSDKMQEQARFAVVNRYTEEQIRDNIFKVMQELEIPAKRDAIKILATNSL
                     VKISMEYTVPVDLLFYHMDLHFAPRSENKALF"
     gene            7268..>8378
                     /locus_tag="VGR03_10695"
     CDS             7268..>8378
                     /locus_tag="VGR03_10695"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEV1994790.1"
                     /translation="MRIRVQKGFFTSAIGLTILGTAFAIFLAAGGVFTYYYIKYSRMI
                     DARLSGNVLQNTTQIFSAPEHISVGQAWGPEDLTAYLTRVGYRPLQDASAIGQFTVQE
                     NTVDIRPSKLSYFAGINALAVQFRGKSIRSIKPLAGGGDLDTAEIEPELITNLFDSAR
                     EKRRPVRYEDLPVMLVDAILSSEDKRFFEHGGFDFIRIVGAAWADVRHSSQHYQGAST
                     ITMQVARTFFLSTDRNWRRKLAEAMISVELEQRFNKQQIFELYANEVYLGNRGSFGIR
                     GFSEASVAYFGKDLRQLTLPECAYLAGIIRAPNYYSSADRHPERGVQARDRVLPQMLE
                     NKYINEEDVQDAKRAALKIIRTSISGSEAPYFVDMV"
BASE COUNT         1770 a         2460 c         2468 g         1680 t
ORIGIN      
        1 cgagaccatc atcatgtaca tcggcgggta cgacgatatg gtggtggcca agacacagat
       61 tcgcgggcgc atcgaagcgg acaacgccaa tcgcgagaaa aaaatcgcgc agttgcagga
      121 gtttatcgcg cggttttccg cgggcacgcg ctccgcgcag gcgacttcgc gcaagaaaga
      181 agtcgagcgc ctgcagacgg cggagctggc gagaagcaat attcagcggc cgtacattaa
      241 gttcgatatg aagcggaact cggggcgcca tccgctggag attcgcagcg tcgagaaatc
      301 ctacgaggat ctaaaagtaa ttccgcgttt ctcggcgagc gtggtgcgcg gcgaaaaaat
      361 cgcgctaatg ggccgcaacg gcgccggtaa aaccactttg ctgaagtcgc tggttcgcaa
      421 cgcgccggga ttcatcgagg acaaggacct gcagtttccg atcgatggcg gcgaggtgag
      481 atggggacac gaagtggcgg tgggttactt cgcgcaggac cacggcgaat ccatcaccaa
      541 aggcatgacc gcgatcgagt ggctttggca gttcgatccc gctgcttcgc aacaggagct
      601 gcgcgggttg atggggcaaa tgctattcag cggcgaagat gcgctgaagc cgacccgggc
      661 gctttccggg ggcgagtccg cgcgactgat tttttgccgg ctgatgctgc agaagccaaa
      721 tttcctggtg cttgacgagc cgaccaacca cctcgacctg gaatcgatca acgcgctgaa
      781 tatcgcgttg cagcggtacg acggaacggt gctgctcgtg acgcacgacc acgacgtcat
      841 cgacgaagtt gccacgcgca tctggcattt ccaaggcggc aaagtcgagg acttcaaggg
      901 tccttatgaa gagtacgttg cctacgcgga acagcaggct tcttaagaac gccatcttca
      961 aaatccttct ttgctttcat aacgcgcgcc ccgcggcggc acccgacgcc cacgcccatt
     1021 gaaaattgaa cccgccgaga tggccggtga catcgacgac ttcgccgatg aaaaataatc
     1081 ctttgacgtt cagacactcc atcgtcttgc tcgacaattc ggcggtatct acgccgcctg
     1141 ccgtcacctc cgccttttcg tagccctcgg tgtccgcggg cttgatcccc cagtcgtgca
     1201 cctgctcctc ccattttgtc agggcatcat cgttccaggc ggcgggggca tggttttcca
     1261 gccagcgttc ggcgaatcgc ttcggcagaa ttccctgaaa cgctttttgc gccgcggcca
     1321 tattgcgcac tttcgccgcg cgaatggcta cggtgatatc ctgatccgga gccagatcga
     1381 tctggatgga ctgccccttc tcccaatagg aagagatttg cagtatcgct ggcccgctca
     1441 aaccacgatg ggtgatcagc attttttcgc ggaaggcgtg ctgatcagtt ttcgcgacca
     1501 cttctgccga gacgcccgcc aagtcgcagt accggctgcg atctccgcca gtcaggacga
     1561 agggcacaag cgcgggcttg gtttccacga ttttcaaacc gaattgctgc gccagttggt
     1621 atccgaaggg ggtcgcgccc atttttggga tcgaaagtcc gcccgtcgca actacgagtg
     1681 ctggggcctg aaattccccg gattcggtgc ataccacgaa ctccgtggtg cgctgcactt
     1741 cccggactcg gctctgtaga aaagtctgca ctcccgcagc ctggcactcc gattcgagca
     1801 agtcgatgat gtcctgcgcg gaacgatcac agaagagttg cccaagcgtt ttttcgtgat
     1861 aagggatgcg gtacttttcg accagcgcga tgaaatccgc tggggtgtag cgcgccagcg
     1921 cggatatcgc gaagtgttcg ttcgcggaaa tgaaattttc cggttggcaa tgaatgttgg
     1981 tgaaattaca gcgccccccg ccggagatca ggattttctt gccgacgcga tcggcgtgct
     2041 cgatcacggc aacacgcctc ccgcgttcgc cggcctcgat cgcgcagagt agtcccgcgg
     2101 cgccggcgcc aagaactacg gcgtcaaatt tgcgtatcaa gaatcaatct cacttgggtc
     2161 ggaatccgct gtctgccgag tttccactgc catgactctc aaggttaaac tatgtgcagg
     2221 agaagaccga gaaaattgcc tccgattatc agcgcgcaag gactgtcgaa gaggtacggc
     2281 gtcgcgccgc tcttccagaa tatttcgttt accgtttccg aaggcgaccg cattggagtt
     2341 attggtccga atggttcggg caagtcgacg ctgctggaga tgttcatcgg ccgcgtcaaa
     2401 ccggacggcg gggatgtagc ggttcgcaaa gggacgcggc taagttacgt cgcgcagatt
     2461 tcggaattcg ctgcgggcgt gacgattcgc tcggtgatcg aaagcgctct ggaacgcacc
     2521 gcggttcccg aaaccgagcg cgcctcacgt tttgccgaaa cgctgggccg cgcgggtttt
     2581 acggatcttg atgcgcacgc cgcgacgctc tccggcggtt ggcgcaaacg actcgctatc
     2641 gccgaggctt tggtgcaggc gccgggcatt ttgctcctgg acgagcctac caaccatctg
     2701 gatctcgcgg ggattcagtg gctcgaatcc gttctccgga acgcggcgtt cgcgtgcgtg
     2761 ctggtcagcc acgaccgcta cttcctggaa aacgtcgcca acgaaatggt ggagctgaac
     2821 cgcgcgtatg aagatggcgc gctccgcgtg aagggaaatt acagcgcgtt cctggaggct
     2881 gaggagctgt accttcacgc gcagaaaaag cggcaggaat cgctggagaa ccgcgtacac
     2941 acggaactcg agtggttgcg ccgcggaccg aaggcgcgcg ccaccaaagc gaaggcgcgc
     3001 attgataaag cccacgagat gatcggcgaa ctcgcggaga tgaatgcgcg cagccgcacg
     3061 tccagcgccg aaatcgattt ctccgcgacg aaccggaaga ccaagcagct catcaacctc
     3121 gaggaggttt cctacggcgt cggcagccgc acgctattca aagagattca tttcaatgtc
     3181 acgtcgggga tgcgagtagg gctggtcggt ccaaacggca gtggcaagac aacactgctt
     3241 cgcctgatac gcggagatat caaaccggaa tccggcaagg ttcgccgcgc cgagggcttg
     3301 cgagtcgtgt acttcgacca gaaccgagag cttgatccgg acattacttt gcgccgggcg
     3361 ctcgcgccgg acagcgatgc ggtgatttat caggagcgcg tcatccacgt cgctgcgtgg
     3421 gccgaacgat ttcttttttc ggctgaaaat ttgaatcagc gggtcggacg gctttccggc
     3481 ggggagcgcg cgcgcgtttt gattgctcag cttatgctgc aacccgctga cgtattgctg
     3541 ctggacgaac cgaccaacga tctcgatatc cccacgctgg aaatcctgga agagagcctg
     3601 ctcgaatacc ccggagctct ggtgctggtc acgcacgacc gcttcatgtt ggatcgcgtt
     3661 tcgacggtgg tggttgggct ggatggcttt ggcgcggcgg agcgttttgc ggattactcg
     3721 caatgggaaa cctggcagcg cgcacagttg gcaatggctt cagccaaatc agcggacgga
     3781 aaccggcgcc ctcgctctgt cgcggacgaa gctccgtgta ccgctgcaaa aaagaaactt
     3841 tcctatgccg aagcccgcga attcgcagcc atcgaaggcc gcatccatga agcggagctg
     3901 atgctgcaag ccaggcgtgc cgcgctcgaa gatcgcgcga tcagcagcga tcgaatgagt
     3961 cttcagaacg cgtgcagcca attggacgaa gcgcagaaat cggtggatgc gctctacgcg
     4021 cgctgggcgg agctcgaaca aaagcagggg tgaccctccg cgatgaccgc gagatgtcgg
     4081 tgccgtgcat ttcccgcgat ccgcagcagg cgcatcacat gacgctgatc tctactcgtt
     4141 tttatttgcc aagcaaactc ttccagcgca cccaagcgtc ggcgcgcgcc ttcttgtcgg
     4201 catctttcgc gtcgggggct tcgcccgcgc gcatgaagcc gtgtcctgcg ccttcgtagg
     4261 tcacgatgtc gaaagtcttg cccgccgctt tcatctgcgc gacggcatcg gggatcaggg
     4321 cgccaatgcg ggcgtcgttt cccgcataaa agccgtagat cggagcctta atgcgcgcca
     4381 tcgcatcctt atccggtggc gggccgtaga acacgaaagc ggccgccaga tcggcgcgat
     4441 tggtggcaaa acggaaggtc tggccgccgc cccagcaaaa gccgccgacc aagagcttcc
     4501 cgttcgaggc agggatcttc attgcgtagt ccgctgcggc atttaaatcc gccgtcacct
     4561 ggtcgggatt caggtggctt atggcttcca tcgttttgcc ttccgcaaaa tcgctgctgc
     4621 ggccgccatt cggtcccatg ccggaaagga gatcgggcgc aaccgcgatg tagcccgccg
     4681 cggccacctg gtccgccaag tcctgcaccc agtccgtcat gccaaagatt tcgtggatga
     4741 tcagcacgac cggggtcttg cccttggatt cgggatagac gacaaatgtt tcgaccgagc
     4801 gcccatcgtg cttgatggtg acccactcgc ggtggcgagg agacttttca agtgtcgcgc
     4861 gtgcccagtc ttgcgcggat gcgacttgcg ctgcgcaaag aattagcagt gaaatcaaag
     4921 tgatgcgttt catggcgtgg tttctccctc agagaacgtc gctggccgga cacgaacttg
     4981 aaaacgcccg tgcggaacga gtggcattgt aatgccgcgc ggcaaggtcc gcaatgcgga
     5041 gaaaagttct tcctttctca gtgcatgcgt tgacacgctt gcatgcgtcc gatacgcttt
     5101 tgagtttact tggcttgact tatcgcttct gagaggaacg gacaaaccgc cgcatggccg
     5161 aagaaaagct tcctacccgc gcacaagatt tttccgagtg gtacaaccaa ctggtcctca
     5221 aggcgcagct tgccgattac gcgccggtcc gcggctgcat gatcgtgcgg ccctacggct
     5281 gggcgctgtg ggaaaacatc caacaggcgc tcgaccgccg cttcaaggcc accggccatc
     5341 aaaacgtggc gttcccaatg cttatcccgc gcagcttcat cgacaaggag aagcatcacg
     5401 ttgaagggtt ttcgcccgag cttgccgttg tcaccattgg tggcggggag gaactcgccg
     5461 agcctctggt gatccgcccc acgtcggaga ccatcatcgg ccatatgtgg tccaagtgga
     5521 tccagtcgta tcgcgatctg cccgttctca tgaatcagtg gaacagcgtg gtgcgctggg
     5581 aattgcgcac gaaacttttt ctgagaacgc tcgaattcta ttggcaggaa ggccacaccg
     5641 cgcacgcgac tcgcgaagag gccgaagcgg aaacgctcca gatgctggat atttacgcgg
     5701 acttcgccgt gaacgacgcg gcgattccgg tgatccccgg gaaaaaatcc gaagccgaaa
     5761 aattcgccgg cgcgcatacc acctacgcca tcgaagcgat gatgggggac ggcaaagcgc
     5821 ttcagtcagg cacgtcgcat tttctgggac agaatttcgc ccaagccttc gaggtcaaat
     5881 acctcgacca gaacggccag ctacagcatt gctggacgac atcgtggggg ctctccacac
     5941 gcatcattgg cgccatcatc atggtccacg gggacgatca ggggcttgtc ctgccgccga
     6001 agcttgcgcc ttatcaggtc gtcatcgtcc cgatcttcaa gacagacgaa gaaaaagcgt
     6061 ccgtcctcga caccgcgcgc aaactgaagg ccgagctgct caaagccaac atccgggtca
     6121 ctctcgatga acgcgaaggc cagagccccg gctggaaatt taatgattgg gagatgcgcg
     6181 gagtcccgct gcgcgtggaa ctcggaccca aggatgttgc caaacaatcc gccgttctct
     6241 cgcgccgcga ccgtccaggc agggaaggaa agatcagcgc ttcgcttgcc ggcctgccca
     6301 ccagcatcga ggggctgctg gtggagatcc agcaatcgct tcacgacaag gccttggcgt
     6361 tccgcaagag caacacacac gatgcgaaaa cctacgacga cctgaagaag gccgtcgaga
     6421 atggctttgc cttctccttc tggtgcggga gcgccgattg cgaagccaaa atcaaggaag
     6481 agacccgcgc caccatgcgc tgcatccccc tggagcagga gggaggaggc gggacttgcg
     6541 tttactgcgc ccagcccgcc acggcgcgcg cgatctttgc ccgggcctac taggcccgtt
     6601 cctctccggg aacatgtccc gcgccaattt ctcgacactc gttcgcgcgg atgctagctt
     6661 taggaagtcg aggaatcacg catcgccgtt cccagccgcg ggcgccctgc ggcacgaacg
     6721 gaccaaggga gatgaatcta gtgcatcttg aaggcatccg agatcgccgc gcgctggcgc
     6781 actgccgctc cgaacgggga gaaggcaagc tcaaggccat catttacctc gcgatcttgc
     6841 tgctcgttat ctattccgca gtcaagatcg tgccaatcta cgtttcgaac tatcagcttt
     6901 ccgacaaaat gcaggaacag gcgcgcttcg cggtggtgaa tcgctacact gaagagcaga
     6961 ttcgcgacaa tattttcaag gtaatgcagg aattggaaat tccggccaag cgcgacgcca
     7021 tcaagatact cgccacaaat tccctcgtga agatctctat ggaatacacc gttcccgtgg
     7081 acttgctgtt ctaccacatg gatcttcatt ttgccccgcg cagtgaaaac aaggccctct
     7141 tctgaggcat cggcgccgcg gctgcccgtt ttcgattctt tgccggcaac ccggcatcga
     7201 ggaactccgc ctttcccaga gtgcggtctt gcgagacaat aagagttcag ccctcgggag
     7261 ctaaggtttg cgcatccgcg ttcaaaaggg attctttact tcagccatcg gcctgacgat
     7321 cctgggcacc gccttcgcca tctttctggc cgccggcgga gttttcacct actactacat
     7381 caagtactcg cgcatgatcg atgcgcggct ctccgggaac gtcctgcaga acaccacgca
     7441 gatcttttcc gcgccggaac atatctcggt aggtcaagcc tggggaccgg aagatctgac
     7501 cgcgtatctc acccgcgtgg ggtaccgccc gctacaggat gccagcgcca tcggccaatt
     7561 tacagtgcag gagaacaccg tcgatatccg gccttcgaag ctttcctatt tcgcagggat
     7621 taatgctttg gcggttcaat tccggggtaa gagcattcga tccatcaaac ctcttgccgg
     7681 cggcggagat cttgataccg cggaaatcga accggaactc attaccaacc ttttcgacag
     7741 cgcacgagag aagcggcggc cggtgcgtta tgaagatttg ccggtaatgc tggtggacgc
     7801 gattctttcc tccgaggaca agcgattctt cgaacacgga ggctttgact ttatccgcat
     7861 agtgggagcg gcgtgggccg acgtgcggca cagcagccag cactaccagg gcgcgagcac
     7921 tatcaccatg caagtggcgc gcacgttctt tctctcgacc gatcgcaatt ggcggcgcaa
     7981 gctggccgaa gccatgattt ccgtcgaact cgagcaacgc ttcaataagc agcaaatttt
     8041 cgagctgtac gccaacgaag tttatcttgg caaccgcggg agctttggca tccgcggatt
     8101 ctccgaggcc agcgtcgcct acttcggaaa ggatttgagg caattgacgc tgccggaatg
     8161 cgcgtacctc gcggggatca ttcgcgcgcc gaattactat tcgtccgccg accgtcaccc
     8221 ggagcgcgga gtgcaggctc gcgatcgcgt gctgccgcag atgctggaga acaaatacat
     8281 caatgaagaa gacgttcagg atgccaagcg cgcggcgcta aagattatcc ggacgtcgat
     8341 ttcggggagt gaagcgccct atttcgtgga catggtga
//
LOCUS       DASYVO010000315         5012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_23428, whole genome shotgun sequence.
ACCESSION   DASYVO010000315 DASYVO010000000
VERSION     DASYVO010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23428"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="VGR03_10700"
     CDS             complement(<1..1208)
                     /locus_tag="VGR03_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEV1994791.1"
                     /translation="MSAVPPATGNQTPKNTRESPPSKDLRGLMPYLRRYPGGIVFGLL
                     VVVLMGIVGNVLPLATGVMTDTLAGNTVPFEHSTQTGTPLVLGLNAATLSHSIPFYAP
                     SSRHTLGIYCLIVVLCVAIKGLLSFSARWTLIGVSRDIEFDIRNDLLKRLLILETEFY
                     VRNRTGELMSRATNDLNAVRMVLGPGIMYSATTIITMIMAVLVMVTLSPTLTLWVLLP
                     APVVAVAVWFFGKTIHDLYEKIQAALATLTARVQENLAGVRVVRAYAQEEAEIRGFDE
                     PNREYVSRNIRLIRTWSMFMPSLQALIGTTFLIVLWQGGHQLLRGQISLGALIAFNGY
                     LAVLVWPMIALGWVTNIFQRGAASMGRLNYILTAQPNIDNRNAKVAEGTKPRGEIEFR
                     HLTFAYPTTLS"
     gene            complement(1205..1903)
                     /locus_tag="VGR03_10705"
     CDS             complement(1205..1903)
                     /locus_tag="VGR03_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994792.1"
                     /translation="MEKTSQSDCSGANEAGIGPSFLKEGPGIGAVRRWRIPLKFSLSF
                     LSLAIFLGGCGADAPKPAAQAETKPAEPAVPEEIQLAAKSLLGSESQVLAFGDLAKNG
                     KQEFLVANVVPKTPTNNLPGTIVTRVVVAENTEGKWAEVLRCDEHLKNQKGFLGLTPL
                     VPVTGWRLQYEQNDEKGLQLYFTPLKGAIDSHVLPIGVRWNPATKRYQSLDRTYEHFL
                     LESPSMHDARSTLR"
     gene            complement(2098..2778)
                     /locus_tag="VGR03_10710"
     CDS             complement(2098..2778)
                     /locus_tag="VGR03_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994793.1"
                     /translation="MANHAYLRVWTRDFSLETMLAEFARFLTTAPLSATQNAFSELIV
                     QAVDPTEIPVAEWDLRPLKTGPAEVAALAVQHLNADTAYIASAQWDLWGFDIESLKWQ
                     HKPEPLVLACHGQEYDNGLAATAGHFMADLGFEHFFTGHGGLLAPEAAANPFDSSDHP
                     MEHTFRQWMAASGNLKEYHAKTRENIQQLFRWVEAIEQALPVERSELWSEGEENFEAR
                     LDAILAQR"
     gene            complement(2897..>5012)
                     /locus_tag="VGR03_10715"
     CDS             complement(2897..>5012)
                     /locus_tag="VGR03_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994794.1"
                     /translation="ERLLSFEQWEQKFAAGRAEIANETTQAIEKFQHEADEHSRTAHA
                     AAAEALRTELPRWLAPQLEQLTRDLTARLSQEGAAQRNEHAQQMESTAETLRTVRREA
                     EETAARLKTQSERSEAQIVARAETAARALEEAAKQREETATAQREALSVAANEIQQQV
                     TAALSSAQSSTHGQLASELEAAQARWRAAIESTLAEAQDRAAGGLNEHARGLVTQWQD
                     EIARHATAARDSATSAATEAEQRMTTLRDALQGHTQRLESALAHAGETSQRLEQHSAQ
                     LENVQQQALAGFQSQIENVLSPHREEMQRRSEANVEEINAKIRATFEEASRQAVAQFD
                     RQIAEMVQPQVTRAEEAVHRLAGGRSLLDAALTLQQDRIRNSADEAFAESLARFRENL
                     GSVEQILQDSSQTITGLNLAELEGKAGDLKHQMVEELLKAAEWYEKKAQTQIQNLTER
                     TIEHAGNQLREKAGEISSVFAGELDHSSRSFVGHAQTQMEEVVRDSFERARALFAEAA
                     DTTAAAFTDEVQRTGRQELEGFNVEVRRSTDGARLQMDAARMELAQKTTAEQESFLQR
                     FQSSMGGALEAGVAEAQKKVSEGFGPLLDSWKAMTVAHQQEMQSIYGQMAEHAAEHYT
                     NRLENVSNQWMLATVASLDHQSREMISGIAATAEEKLREACAQVFAGVGESLRERLRE
                     MAQSFTPPTEPPARAKSAKTGS"
BASE COUNT          866 a         1604 c         1440 g         1102 t
ORIGIN      
        1 gacagagtgg ttggataggc gaaggtgaga tggcggaatt caatttcgcc gcgaggcttg
       61 gtgccttccg caactttggc gttgcgatta tcgatattcg gctgggccgt gaggatgtaa
      121 ttgagccggc ccatggaggc cgcgccgcgc tgaaagatat ttgtaaccca tccaagagcg
      181 atcatgggcc agacgagcac ggcaaggtag ccgttgaagg caatgagcgc accgagggaa
      241 atctggccgc ggaggagctg atggccgccc tgccacaaca cgatcagaaa ggtcgtgcca
      301 atgagcgcct gaagcgaggg cataaacatg ctccaggtgc ggatgaggcg gatgtttcgc
      361 gagacatact cgcggttggg ctcgtcgaag ccccggattt ccgcttcttc ctgggcgtag
      421 gcgcgcacga cacgcacgcc ggcaagattt tcctggacgc gcgcggtgag cgtggcgagc
      481 gccgcctgga ttttctcgta caggtcatga atcgttttgc caaagaacca taccgcgact
      541 gccacgaccg gcgcgggaag tagaacccag agcgtgaggg taggcgagag cgtgaccata
      601 accaggacag ccatgatcat ggtgatgatg gtcgtcgcgc tgtacatgat gccggggccg
      661 agcaccatgc ggacggcgtt caggtcgttg gtggcgcgcg acattagctc gccggtgcga
      721 ttgcgcacgt agaattcggt ctcgagaatc agcaggcgct tcaggagatc gttgcgaatg
      781 tcgaattcga tatcgcggga tacgccgatg agcgtccagc gcgccgagaa agaaagaagg
      841 cccttgatcg cgacgcataa aacgacgatg aggcaataga tgcccagcgt gtggcggctg
      901 ctcggcgcgt agaacggaat ggaatggctg agggtcgccg cgttcaggcc caagacgagc
      961 ggagtgccag tctgagtgga gtgttcaaag ggaaccgtat tgccagcgag cgtgtccgtc
     1021 atcacgccgg tggcgagcgg gagcacgttg ccgacaatgc ccatgagcac gaccacgagc
     1081 aggccgaaaa caatcccgcc ggggtagcgc cgtaggtacg gcatcaggcc gcgcaggtcc
     1141 ttactgggag gagattccct ggtgttcttg ggcgtctgat taccggtggc tggcgggacc
     1201 gcgctcatcg cagcgtcgac ctcgcatcgt gcatggatgg tgactcgagc aggaaatgct
     1261 cgtaagtccg gtcgagcgac tggtagcgct tcgtcgccgg attccagcgc acaccgatgg
     1321 gcagcacgtg cgaatcgatg gcgcctttca gcggtgtgaa ataaagttgc aggccctttt
     1381 catcgttttg ctcgtactgc agccgccagc cggtcaccgg caccaacggc gtcaaaccca
     1441 gaaaaccctt ttggtttttc aggtgttcat cgcagcgcag gacttctgcc cattttcctt
     1501 cggtattctc cgctacgacg acgcgcgtca cgatcgtccc cggcaaattg ttggttggcg
     1561 tttttggcac gacgttcgcc acaagaaatt cctgcttccc gttcttcgcc aggtctccga
     1621 acgccagcac ctgcgactcg ctccccagca gcgactttgc cgccaactga atttcttcgg
     1681 gcacggccgg ctccgcgggc ttggtttccg cttgcgccgc aggcttggga gcatccgcac
     1741 cgcacccgcc cagaaaaata gccagggaca ggaaactaag tgagaatttc agcgggattc
     1801 tccatctgcg gaccgcgcca ataccggggc cctcttttag aaaggacgga ccgatacccg
     1861 cctcgttagc acccgaacag tcgctttgcg aagttttctc catcagtgat ttgccgccgt
     1921 agtcgtgaac tcgaccactc gcccgggctt cctatcattc tccagcgtgc agagcacttc
     1981 catcacctgc ggcacatccg gacccacccc atcaaatgcc tgctacagca attctagctg
     2041 ttgcccgccc ttcgtggaag gaccccgtga atcgggcttg cccaagattt gaatgcgcta
     2101 ccgctgcgcc aaaatcgcgt ccagccgcgc ttcaaagttt tcctctcctt cagaccaaag
     2161 ctcgctgcgc tccactggca gagcctgctc gatcgcttcc acccaacgga acaattgttg
     2221 gatgttctcc cgggtctttg cgtgatattc cttcaagttg ccgctcgcgg ccatccactg
     2281 ccggaacgta tgctccatcg gatgatccga cgaatcgaaa ggattcgccg ccgcctcggg
     2341 cgccagaagc ccgccatgcc ccgtgaaaaa gtgctcgaag cccaagtcgg ccatgaagtg
     2401 tccggccgtc gccgcaagcc cgttgtcata ttcctgcccg tggcacgcca gcaccagcgg
     2461 ctccggcttg tgctgccact tcaggctctc gatatcgaag ccccacaagt cccattgcgc
     2521 gctcgcgata tacgccgtgt ccgcgttcag gtgctgcacc gccagcgctg caacctccgc
     2581 aggtcctgtc ttcaacggac gcagatccca ctccgccacg ggtatctccg ttggatccac
     2641 ggcctgcacg atcagttcgc taaaggcatt ctgcgtcgcc gaaagcggcg cggtcgtcag
     2701 gaaccgcgcg aattcggcaa gcatcgtttc cagtgaaaaa tcgcgcgtcc acacgcgcag
     2761 gtaagcatga ttggccatgg tggttggaac ctttgggatt ataccgagcc agcagggaac
     2821 tggcgaatca gcagccggag tgtgttgagc gtcgttgccg tggcacagcc attcttggct
     2881 gtggaggttt tggttttcag cttcccgttt tggcgctctt tgcgcgcgcg ggaggctccg
     2941 tcggcggcgt aaaactctgc gccatctccc tcagccgttc gcgcagggac tcgccaacgc
     3001 ctgcgaatac ctgggcgcaa gcttcgcgaa gtttttcttc cgccgtcgcg gcaattcccg
     3061 aaatcatctc ccgcgactgg tgatccagcg acgccaccgt cgccagcatc cactgatttg
     3121 aaacattttc cagccggttc gtgtagtgct ccgcggcgtg ttcggccatc tggccgtaga
     3181 tgctctgcat ttcctgctga tgcgcaaccg tcatcgcttt ccaggaatcc agcagcggcc
     3241 caaatccttc agatactttc ttctgtgctt cggccacgcc cgcttcgagc gctccgccca
     3301 tggaggattg gaaccgctgc aggaaacttt cctgttcggc cgtcgtcttc tgcgccagtt
     3361 ccatacgtgc cgcgtccatt tgcagccggg ccccatccgt ggagcgccgc acctctacgt
     3421 tgaatccctc cagctcttga cggcctgtgc gttgaacctc atccgtaaac gccgccgcgg
     3481 tcgtgtccgc tgcttccgcg aaaagcgcgc gcgcgcgctc gaaagaatcc cgcaccacct
     3541 cttccatctg cgtctgcgcg tgtcccacga aactgcggct tgaatgatcc agctcgcccg
     3601 cgaagacgct ggaaatctct cccgctttct cccgcaactg gtttccggcg tgctcgatgg
     3661 ttctttccgt cagattctga atctgcgtct gcgctttctt ttcgtaccat tccgccgctt
     3721 tgaggagttc ttcgaccatc tggtgcttca aatcgcccgc ttttccttcc agttcggcca
     3781 ggttgagtcc ggtgatcgtt tgcgaggagt cctgcaggat ttgctcgacg ctgcctagat
     3841 tctcccggaa acgcgccagt gattcagcga acgcttcgtc cgcggagttg cggattcggt
     3901 cctgctgcag cgtcagcgcc gcatccagca gcgaccgccc ccctgccagc cggtgaaccg
     3961 cttcttcggc gcgcgttacc tgtggctgca ccatctccgc gatttgccgg tcaaactgcg
     4021 ccaccgcctg gcggctggcc tcttcgaatg tcgccctaat cttcgcgttg atctcctcca
     4081 cgttcgcttc ggaccggcgt tgcatctctt cgcggtgcgg gctcagtaca ttttcgattt
     4141 gcgattgaaa tccggccagt gcctgctgct gtacgttttc aagctgcgcc gaatgttgtt
     4201 cgagccgctg gctcgtctcc ccggcatgcg ccagcgccga ctccagccgt tgcgtatgcc
     4261 cttgcaacgc atcccgcaac gtggtcattc gctgctcggc ctctgttgcc gcgctcgtcg
     4321 ccgaatcacg tgctgccgtg gcgtgacgcg ctatctcatc ctgccactga gtcaccaacc
     4381 cgcgtgcgtg ctcgttcaat ccgcctgccg cgcgatcctg ggcttctgcg agcgtactct
     4441 cgatcgccgc ccgccaccgc gcttgtgccg cttccaattc actcgcaagc tgtccgtgcg
     4501 tggacgattg cgcggacgag agcgccgctg ttacttgttg ctgaatctca ttggctgcca
     4561 cgctcagcgc ctcgcgttgc gcagttgccg tttcttcccg ttgcttcgcc gcttcctcaa
     4621 gtgctcgcgc cgccgtttcg gcgcgcgcaa caatttgcgc ttccgaccgc tccgactggg
     4681 tcttcaaccg ggcggccgtt tcctctgcct ctcggcgaac ggttcgcaga gtctccgccg
     4741 tgctttccat ctgttgggcg tgctcgttgc gctgggctgc gccttcttgt gagagcctag
     4801 ccgtcaggtc gcgcgtgagt tgctcaagtt gtggcgccag ccatcgcggc aattccgttc
     4861 gcagcgcctc ggccgctgct gcatgggcgg tccgtgaatg ttcatccgct tcgtgctgaa
     4921 acttttcaat ggcctgcgtg gtctcattgg cgatttccgc gcgtccggcc gcaaactttt
     4981 gctcccactg ctcgaacgat agcagccgct cc
//
LOCUS       DASYVO010000316         4050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_78289, whole genome shotgun sequence.
ACCESSION   DASYVO010000316 DASYVO010000000
VERSION     DASYVO010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78289"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VGR03_10720"
     CDS             <1..343
                     /locus_tag="VGR03_10720"
                     /inference="COORDINATES: protein motif:HMM:NF039244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5818 domain-containing protein"
                     /protein_id="HEV1994795.1"
                     /translation="RGGDEKTFKGEIADTQCALNVHSLSQSHKEMIEMKPEVKTNADC
                     ARYCVKERGGRFVLQTPGKVYKLDAQVLAEQWAGLKVKVIGILDPKTDIITVRGIEPI
                     FSTPSKTASPK"
     gene            697..1137
                     /locus_tag="VGR03_10725"
     CDS             697..1137
                     /locus_tag="VGR03_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994796.1"
                     /translation="MSLRGNCPAKVDEKGRLKIPAVFLDALKEYGNQFYITSTTGESA
                     RIYPMKVWSGIEDKLAGVSSQNRAKRKFLMRTSYYGQTVELDGQGRVLVPAVLRESAQ
                     MKGEVDVFGNLDYLEVMNHTRVLDDLKNSPYTEEDDKALEDLGI"
     gene            1150..2037
                     /gene="rsmH"
                     /locus_tag="VGR03_10730"
     CDS             1150..2037
                     /gene="rsmH"
                     /locus_tag="VGR03_10730"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV1994797.1"
                     /translation="MAAPHTPVLLEEVLEWLRVRPDGTYIDATLGAGGHSEAIAKRLT
                     SGRLISLDRDAQALELARERLKSFGETVKLVQSPFSRIAEVAQEWGIPPVDGVLADLG
                     VSSMQLDQGARGFSFREAAPLDMRMDAANDELTADEIVNRWSEKEIADLLYREADEHD
                     SRRIAKAIVRARPLRDTAHLATTVAGARKQWGRQRLHPATKTFLALRIAVNREMEELG
                     QFLSRTPATMNSGGRWVVLSYHSREDRLVKHAFQEGERAGTLRVLTKHVIQPSEQEMM
                     GNPRSRSAKLRCAERTPAA"
     gene            2057..2473
                     /locus_tag="VGR03_10735"
     CDS             2057..2473
                     /locus_tag="VGR03_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994798.1"
                     /translation="MARKKKLPAMTEFHTVKRIDNSRIVRHIEPVKMRKLYRTVALGG
                     VVAAFFMLYISQHFRCIDLSFQLEDVKARQAETASLNSSLKLEIAGLRNPMRIDVIAR
                     RQLGLTEPLPTQVQEYEAPDGAEVAAVRSVRPNRAP"
     gene            2563..3948
                     /locus_tag="VGR03_10740"
     CDS             2563..3948
                     /locus_tag="VGR03_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEV1994799.1"
                     /translation="MPENRVHAAAGGRIAVNSERGRKTVELNGDRKAVSRLLIVACLT
                     LLWMTAVLGRQGYLQLFRHSEYMARAQRQQQRVIEITPKRGAIYDRNMHPLAMSIPVD
                     SAFAVPSELGDQHLAARLLSGILGIPREVLEARLESSHSFVWIARKLPPDKKEAVEAL
                     NLKGVYFQKENQRIYPKRDLASHVLGFVNLDEKGIGGIESELESKIGGKSEKIIVMAD
                     ARQRWFDGGEAQRERGANVVLTLDEKIQYIAERELTAAIAKTHALAGTVIVMNPNNGE
                     ILALANWPKFNPNAATDAPAETRMNRAVSAIYEPGSTFKLITLAAAFDQGITRPEEVL
                     DCENGAVYVAGHRIRDHKPFGLLTVSDILAQSSDVGAIKIALRLGAPKFYDYIRAFGF
                     GQLTGVDIPGESKGMLQHLETWTPVSIGSISMGQEVGVTPIQLINAVSAIANGGMLYK
                     PHVIAELRRGD"
BASE COUNT          943 a         1033 c         1302 g          772 t
ORIGIN      
        1 tcgcggaggg gatgaaaaga cattcaaagg tgaaattgcg gacacgcagt gcgcgctgaa
       61 tgttcattct cttagccaat cgcacaagga aatgatcgaa atgaaacccg aagtgaagac
      121 gaatgccgac tgcgcgcggt actgcgtgaa agagcgaggc gggaggttcg tactgcagac
      181 tccgggcaag gtctacaagc tggatgcgca ggtcctcgct gaacagtggg cggggctgaa
      241 agtaaaagtt attggaatcc ttgacccaaa gaccgacatc attaccgttc gcggtattga
      301 gccgattttc tcaaccccga gcaaaacagc ttcgccgaag tgacgcgcga tgatccgaaa
      361 gttctggtcc gagggatgct aaaaccccac cactaagaat tgttgtagct gaaaaaagaa
      421 accgcaacca gtcgcggtgg aaatgatgta gtacctcttc gctttatctt cacccctatt
      481 tttgaaagaa agtatgattc cgcttgacat ggttggaaag cggcctatta tgtggtgaaa
      541 agtggtaaga agtgggtagt agtagaacag agtagcgggc gctggggcaa agtagacagt
      601 ttgagagagt tgtggtgaaa gtgggcattt tggccattct agaccacatt ttcgtgtgcg
      661 atccaggcaa gaacagggtc acgttgagtg gagaacatga gtctgcgggg caactgtccg
      721 gcgaaggtgg acgagaaggg tcggttgaag atacctgccg tgtttctgga cgccctgaaa
      781 gagtacggaa atcagtttta catcaccagc accacgggcg aatcggcgcg catttatccg
      841 atgaaggttt ggtcgggcat tgaggacaag ctggcgggag tttcgagcca gaaccgggcg
      901 aagagaaagt ttttgatgag aacaagctac tacgggcaga cggtcgagtt ggacgggcag
      961 ggcagagtgc tggtgcccgc agtactgcgc gaatcggcgc agatgaaggg cgaggtggac
     1021 gtgttcggca atctcgatta tctcgaggtg atgaaccaca cccgcgtgtt ggacgacctg
     1081 aagaactcgc cgtacaccga agaagacgac aaggcgcttg aagatttggg aatttagggt
     1141 tttgcaagat tggctgcgcc gcacacgccg gtgctgttgg aagaggtgct cgaatggctg
     1201 cgcgtaaggc cggacggaac gtacatcgac gccacgctgg gagcgggcgg gcactcggaa
     1261 gcgatcgcaa agcggctcac ttccggcagg ttgatcagcc tggaccggga cgcacaggcg
     1321 ttggagctgg cgcgggagcg gctgaagagt ttcggcgaga cggtgaagct ggtgcagtcg
     1381 ccgttctcga ggattgcgga ggtcgcgcaa gagtggggga ttccgccggt ggacggggtc
     1441 ttggccgact tgggagtttc gagcatgcag cttgatcaag gcgcgcgggg attttcgttt
     1501 cgcgaagctg cgccgctgga catgcgcatg gacgctgcca acgacgagct aacggcagac
     1561 gaaattgtga atcgatggtc ggagaaggaa atcgccgacc tgctctaccg ggaagcggat
     1621 gagcacgatt ccagaaggat cgccaaggcg atcgtacgag cgcggcccct acgggatacc
     1681 gcgcacttgg caacgactgt ggcaggggcc cgaaagcaat ggggaaggca gagactgcat
     1741 cccgcaacaa aaacgtttct ggcgctgcgg atagcggtga atcgagagat ggaggaactc
     1801 gggcagtttc tttctcggac ccccgccaca atgaattcag gaggccgctg ggtggtgttg
     1861 agttaccact cgcgggaaga ccgcctggtg aagcatgcgt ttcaagaggg agagcgagca
     1921 ggaacgctcc gcgtgctgac gaaacacgtg atccagccca gtgagcaaga gatgatgggc
     1981 aacccgcgtt cgcgaagcgc gaagctgcgg tgtgcggaaa gaacgccggc tgcttagcgg
     2041 ccgacaaggt atcgttatgg cacgcaagaa aaaattaccg gcgatgaccg agtttcatac
     2101 cgtcaagcgg atcgacaatt cacgtatcgt gcgccacatc gagccggtga agatgcgcaa
     2161 actgtaccgc acggtcgcgc tgggcggcgt ggttgccgcg tttttcatgc tgtacatctc
     2221 ccaacacttc cgctgcatcg atttgagttt tcagctcgag gacgtgaagg ccagacaagc
     2281 cgagacggct tcgctgaact cgtccttgaa attggaaatc gccggactgc gcaatccgat
     2341 gcgcattgac gtgattgcgc gccgccaact gggcctgacg gagccactgc cgacccaagt
     2401 gcaggaatat gaagctccgg acggcgccga agtggccgcg gtacggtccg ttcggccgaa
     2461 tcgcgcaccg tagtcttttt tcccgatgca catggctcgg caaccagttc gctgtcaggc
     2521 aaatgaactt ccaggcggag cgcttgtctt tctgatttcg ctatgccaga aaaccgtgtc
     2581 cacgccgccg caggtggccg catagcggta aattccgaac gtggccgcaa gacggtcgaa
     2641 ttgaatggtg accgcaaagc ggttagccgt ctgctgatcg ttgcttgctt gacactctta
     2701 tggatgaccg ccgttcttgg gcggcaggga tacctgcaac ttttccgtca cagcgaatac
     2761 atggcccgag cgcagcggca gcagcagcgc gtgatcgaaa tcacgcccaa gcgcggcgca
     2821 atctacgacc gcaacatgca cccgctggcg atgagcatcc cggtggattc ggcatttgcg
     2881 gtgccctccg agcttggcga ccagcatctg gcggcgcggc tgctctccgg catcctggga
     2941 attccacgtg aagtgcttga agcgcgactc gaatcttcgc actcgtttgt atggattgcg
     3001 cgcaagctgc cgcccgacaa aaaggaggcg gtggaggcgc tgaatctgaa gggcgtctac
     3061 ttccaaaaag aaaatcaacg catctatcca aagcgagatc tggcgtcgca cgtattgggc
     3121 ttcgtcaacc tggatgaaaa aggcattggc ggcatcgagt ccgaactgga aagcaagatt
     3181 ggcggcaaga gcgaaaagat cattgtgatg gccgatgcgc ggcagcgctg gtttgacggc
     3241 ggggaagcgc aacgcgagcg tggcgcgaac gtagtgctaa cgctggacga aaagattcaa
     3301 tacatcgccg agcgagagct gacggcggcc attgcgaaga cccatgccct agccggaacc
     3361 gtcatcgtca tgaatcccaa caacggcgag attcttgctc tcgcaaactg gcccaagttc
     3421 aatcctaacg cagcgacaga cgcgcccgct gaaacgcgga tgaaccgcgc ggtgagcgca
     3481 atctacgagc cgggttcgac gtttaagctg atcacgctgg cggccgcgtt tgaccagggc
     3541 atcacgcgtc ctgaagaagt tcttgattgc gagaatggcg ctgtctacgt ggcggggcac
     3601 cgcatccgcg accacaagcc gtttggtttg ctgacggtgt cggacattct ggcgcagtcc
     3661 agcgacgtgg gagctatcaa gattgcgctg cggctgggcg cgccgaagtt ctacgactac
     3721 atccgcgcgt tcggcttcgg acagctcacg ggtgtggata taccaggaga gagcaaggga
     3781 atgctgcagc acctggagac ttggactccg gtctcgatcg gatcgatctc catgggtcag
     3841 gaagtcggag tcacgccgat tcagctcatc aacgccgtct ctgcgattgc caacggagga
     3901 atgctctaca agccgcatgt tattgcagag ttgaggcggg gggattaagt attgcctgcg
     3961 gaaggcctgc tggcgccgtc cgagcccagg aaagtgatcc ggccggaaac ggcggcgacc
     4021 ctgcggaggc tcatggaagg ggtcatacta
//
LOCUS       DASYVO010000317         4731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_232427, whole genome shotgun sequence.
ACCESSION   DASYVO010000317 DASYVO010000000
VERSION     DASYVO010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232427"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VGR03_10745"
     CDS             <1..470
                     /locus_tag="VGR03_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994800.1"
                     /translation="TNGGVLYAVRTDINNCAQSSAALGVTPLGFPIPIETAALNDDVF
                     FSTSTGVSKIHVVYPLAACAPVTFTVSPGGWVNPAITNPSSLMFTSPPQAEFMYVASS
                     DGHLYKIDPTTGANAANRLINAGATIGDPSFDTVIQKFYVGDSTGHIFSFDLF"
     gene            complement(500..1672)
                     /locus_tag="VGR03_10750"
     CDS             complement(500..1672)
                     /locus_tag="VGR03_10750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1994801.1"
                     /translation="MGNSEAQPRLGRSDREVIVVGGSAAGLFTAASVARGGRRVRVLE
                     SKPQFEPAARTLIVTDHFRHQMGASARESIINEIRRFELFTDGRSAQVALTKPDLIIE
                     RSRLIPALAREAQQAGATLSFDTRFLGIGPNARGLRLEVESGGKHEELHADSVVGADG
                     AASRVARASGWPPIETVPLVQAIVRLPKDCPPDTTRVWFVPDDTPYFYWLIPESAERG
                     ALGVIGEHGSDTKRCFERFLEKKHLEPLEWQGARIPVYRGWVPVRRQIGNGEVYLVGD
                     AAAQVKVSTVGGIVTGFRGALGVSQALLQNGKSRELTALRRELGTHWLIRRALHHFEQ
                     KDYSQLVDLLDASTRQSLGEINRDESTRLLWNVVRRQPRLVLLGLRGLLMGKAERS"
     gene            complement(2018..2518)
                     /locus_tag="VGR03_10755"
     CDS             complement(2018..2518)
                     /locus_tag="VGR03_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEV1994802.1"
                     /translation="MSSKGLFSLGNVTEGEYLFKVESDDRAPVYGAVHLLGDGAHEIK
                     VIMLAVATGTTESVGGGSALRDAVRPPRDPAKPPKVKIPTLKKKVTPVYPDAARRAGV
                     QGNVRIATIMLPDGTLYDLVVLSAPNRDLAVAALAAVRQWRYSPTQLDGEPVETNFTI
                     DVKFDR"
     gene            complement(3001..4053)
                     /locus_tag="VGR03_10760"
     CDS             complement(3001..4053)
                     /locus_tag="VGR03_10760"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEV1994803.1"
                     /translation="MAAGLLAFFIALFASLTLVVPVRALALRVGMVDLPGPRKVHLTP
                     IPLLGGLAMYAAVVIAVLFAFEGPARAQIVGILIGATLVAGVGILDDRGLLHHQVKLF
                     VGMPLAAAILLVSGIRAQVFSVLVGGRSGDFLDAVLTVVWVVGITASFSILDHMDGLC
                     AGVAAMASVFFAMLAYLNGQTLVTTLAAAVLGAATGFLRWNFKPAKIFMGDGGAMFLG
                     YLMATLGLKLRLENSNHLSAWLAPILILGVTIFDTTLVTISRSRRGLLPFATPGKDHA
                     AHRLSNLGLGHRGAVLMMYLLGAIGGGAAVLVSYLSELKAFFVGALALGIIFAGVAYL
                     ERAPYERQIRKTSPVS"
     gene            complement(4257..>4731)
                     /locus_tag="VGR03_10765"
     CDS             complement(4257..>4731)
                     /locus_tag="VGR03_10765"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV1994804.1"
                     /translation="SCRYFTVYGPRGVENHAVIAMIARAFIGQNPFEVWGDGTQIRNW
                     TYIEDIVEGTILAGEKISDGTAINLGTMERIRVIDCANMVCEFTGHKAEIKLLRNMPT
                     GPLNRVADNSLAKKLLGWEPKVLFREGLKRTIDWYYATRDKEEVKRIFTRMLTER"
BASE COUNT         1007 a         1419 c         1369 g          936 t
ORIGIN      
        1 ccaccaacgg cggggtgctc tacgccgtgc gcacggatat caacaattgc gcccagagca
       61 gcgccgctct cggcgtcact ccccttggct tccccattcc cattgaaacc gcggccttga
      121 acgatgatgt tttcttttcc acttcgaccg gggtatccaa aattcacgtg gtctatccgt
      181 tggcggcatg cgctccggtc acgtttactg tttcacccgg cggatgggtg aatcccgcga
      241 ttacgaaccc ttcgtccctc atgttcactt ctccgccgca agccgaattc atgtatgtcg
      301 cttcctccga cggacacctt tacaagatcg accccactac cggtgcaaat gccgcgaatc
      361 gcttgatcaa tgccggtgcg acgatcggcg atccgagttt tgacaccgtt attcagaaat
      421 tctatgtggg tgactcgacc ggccacatct tctcgttcga tttgttttaa gcgatcgggc
      481 agggaatcgc aggcttcttc taacttcttt cggctttgcc catgagcaag ccgcgcagcc
      541 cgagaagcac caggcgtggc tggcggcgca ctacgttcca caatagacga gtggactcat
      601 cacggtttat ctcgccgagc gattgccgcg ttgaagcgtc cagcagatcc accagctgtg
      661 aataatcctt ctgctcaaaa tggtgcagcg cgcggcgaat cagccagtgc gttcccaatt
      721 cacgacgcaa ggccgtaagt tcccggctct tgccattctg caaaagtgcc tgcgagactc
      781 cgagcgcgcc gcggaaaccg gtaacgattc cccccacggt cgaaaccttg acctgcgccg
      841 ccgcgtcacc caccagataa acttcgccgt tcccgatttg ccgccgtacc ggcacccagc
      901 cgcgatagac cgggatgcgc gcgccttgcc attcgagcgg ctccagatgc ttcttttcga
      961 ggaagcgttc gaagcagcgt ttcgtgtcgc tgccgtgctc gccgatcaca cccagcgcgc
     1021 cgcgttcagc cgattcgggg atcagccagt aaaagtaggg cgtatcatcg ggcacgaacc
     1081 agacgcgggt cgtatcggga gggcaatctt tcggaaggcg cacgatggct tgaacgagtg
     1141 gtacggtctc gatggggggc cagccggaag cacgcgccac gcggctcgcc gcgccatctg
     1201 cgccaaccac gctgtccgcg tgcaactctt cgtgtttgcc gccggattcg acctccagcc
     1261 gcaagccgcg cgcattcgga ccgattccca ggaaacgcgt atcgaatgat agcgtcgcgc
     1321 cagcttgctg ggcctcgcgg gccagagccg gaatcaagcg cgagcgctca atgatgagat
     1381 cgggcttggt tagggcgacc tgcgccgagc ggccatcggt gaaaagctcg aaacggcgaa
     1441 tctcgttgat gatgctttcg cgggcggaag cacccatttg gtggcggaag tgatcggtaa
     1501 caataagtgt gcgggccgca ggctcgaatt gcggttttga ttccagcacg cgcacacgcc
     1561 ggccgccgcg ggccacgctc gcggcggtga agagtcccgc agcggagccg cccacaacga
     1621 taacttcgcg gtcgctccgt ccaagccgag gctgtgcttc tgagtttccc atagtttccg
     1681 tgcttcccaa ggacgtgcat tccctgcaaa tactgcccat cagggatccc acgccaagaa
     1741 tttacctgct tcccgctcga cccgtgtggg gctgctcgag gtgtgagtga gagagcgccc
     1801 ggtaagtata gcgaatccac caccgagggc caacacgcgg agagattcgg cagtaggtca
     1861 tcagtttacg aatacgcgac ttatcgggag ttaagcggat tagctttggt tccggacgag
     1921 aaggtctaaa ctcgtcagcc tctcatgaca gacagctcca gtcagtggcc ctgccaaccc
     1981 taggtcaaac gcccgcaatg tccgatctgc cttctaacta acgatcaaat ttgacgtcaa
     2041 ttgtgaagtt ggtttcgact ggttcgccgt ctagctgcgt aggagaataa cgccactgcc
     2101 gcacggccgc tagtgccgcc accgccagat ctctattcgg tgccgatagg accacgaggt
     2161 cgtacagtgt gccgtcagga agcatgatgg ttgcaattct tacgttgcct tgaacgcccg
     2221 ctcttcgtgc cgcatccggg tagacgggag tgaccttctt tttaagcgtc ggtattttca
     2281 ctttgggtgg tttggccggg tcccgtgggg gccgaactgc atctcgaagg gctgatcccc
     2341 ctccgacaga ttccgtagtc cctgttgcca cggcaagcat gatgaccttg atttcgtgag
     2401 cgccatcgcc caacaaatgt acagcgccat aaacgggcgc ccggtcgtcg ctctcgacct
     2461 tgaacaaata ttcgccttcg gtcacgttgc ccaaagaaaa aaggcccttg gaactcaagg
     2521 tttgcagccc tcttccggtc gtggcatccc ataaattgac cttcgctccg tctattggtc
     2581 ctttcgtgct gtcctggagg gttccggata aactcgggag cgcgacagca cacggagcta
     2641 acaaagcgga taggaaaaga gcttttagca tgggacgatc aggatcaagc gattgagaag
     2701 cccagaaaca tcctagacca attctgcgac cttttctaca accgtggcga tctcggggcc
     2761 cgaacctcca ttccggaaac gagggcttga ttgcaattca atctgacgag aaacgcctta
     2821 accgcagaag tgatggcgca cggttccaat cgagttcccg gctacccgga aacagagagg
     2881 aacttgcagg tcagtggcag cgagccatat gcctggtaaa cgcggttacc ggaaactgat
     2941 gacccactcc cagagtttca aaactagcga tggacgatgc tttcgaaccg ctcggttgtc
     3001 ttaggagacc ggggaggtct tacggatttg gcgctcatag ggcgcacgct caagatacgc
     3061 gacgccggca aagatgatgc cgagagccag agccccaacg aagaaggcct tcagttcgga
     3121 aaggtagctg acaagcacgg ctgcgccgcc gccgatggcg ccgagcagat acatcatgag
     3181 gacggcgccg cggtggccca gtccaagatt ggaaaggcgg tgagctgcgt ggtccttccc
     3241 gggcgtggcg aaaggcagca gcccgcgccg cgagcgggaa atcgtgacca gcgtcgtatc
     3301 gaaaatcgtc acgcccagga tcagaatcgg agcaagccat gcggacagat gattggagtt
     3361 ctccaggcgc agttttaacc ccagcgtggc catcaggtaa ccaagaaaca tggcgccgcc
     3421 atcgcccatg aaaatcttcg cgggcttgaa attccagcgg agaaacccgg tggccgcgcc
     3481 aagaacggcg gcagccagcg tggtaaccag cgtctggccg ttcaggtaag cgagcatcgc
     3541 gaaaaaaacg gaggccatgg ccgcgacgcc ggcgcaaagg ccgtccatgt gatcgaggat
     3601 gctgaaggag gcggtgatcc cgacgaccca aacaaccgtg aggaccgcgt ccaggaaatc
     3661 tccgctgcgt ccgcccacga gcacgctgaa aacctgcgcg cgaattccgc tgaccagcag
     3721 gatcgccgcc gccaggggca tgcccacaaa cagtttgact tgatgatgga gcagcccgcg
     3781 gtcgtcgagg atgccgacac ccgcgacgag cgtcgcgccg atgaggatgc ccacaatctg
     3841 ggcgcgtgca ggaccttcaa aggcaaagag caccgcgatt actaccgcgg cgtacatcgc
     3901 caggccacca agaagcggga tcggcgtgag atgaactttg cgcggccctg gcagatcaac
     3961 catcccaacg cgcagcgcca gggcgcgcac gggaacgacc agcgtcagcg acgcaaatag
     4021 tgcgatgaaa aaagcgagca ggcctgcggc catgcaacct cgtttatcag gtgagtcgaa
     4081 tccagccaga ccggtgcggc cggtgtctca cgtgttcctc gccgggttgg ggctccggct
     4141 cccaagtcga gctagcaaat ggctgcccca cgcagcctta acaccatact gcgattttag
     4201 ttgcgcttca atcgggataa tcctgtatgt gcgcagattg tttggaggtc tcgggctcaa
     4261 cgctccgtca acatgcgcgt gaagatgcgc ttcacttcct ccttgtcccg ggtagcgtag
     4321 taccagtcga tggtgcgctt gaggccctcg cggaagagca ccttcggttc ccagccgaga
     4381 agtttcttgg cgagagaatt gtcagcgacg cggttgagcg ggccggtggg catatttcgt
     4441 aacagcttga tctcggcttt gtgtccggta aattcgcaca ccatattggc gcaatcgata
     4501 acgcgtatac gttccattgt tccgagatta atagccgtgc cgtcgctaat cttctctccg
     4561 gctaggatgg tgccctcgac gatgtcctca atgtacgtcc agttgcgaat ctgcgtgccg
     4621 tcgccccaga cttcgaaggg attctgtccg atgaaggcgc gggcgatcat ggcgatcacc
     4681 gcgtgattct ccacgccgcg cggtccatac accgtgaagt agcggcagga a
//
LOCUS       DASYVO010000318         8414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_53495, whole genome shotgun sequence.
ACCESSION   DASYVO010000318 DASYVO010000000
VERSION     DASYVO010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8414
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53495"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VGR03_10770"
     CDS             complement(<1..795)
                     /locus_tag="VGR03_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV1994805.1"
                     /translation="MTNGWVDIKNTDMMLIMGGNPAENHPCGFKWAIEAKRNRNAKMI
                     VVDPRFTRTASVADLFCQIRAGADIAFLGGVIRYAIENNRIAKDYLLNYTNAAFIVKD
                     GFKLPETDGVFSGFDAKSQTYEKSSWNYAGGDASGKPMPEAHAVAAAAHPANGAPPPP
                     PALPEKVAYDFSLQNPNCVFQLLKKHYSRYTPEMVERITGIPRDQFEKAAELFTSVRK
                     DGDMKKAATIIYAVGWTHHTFGTQIIRTAAILQLLLGNVGRAGGGVN"
     gene            complement(844..1425)
                     /locus_tag="VGR03_10775"
     CDS             complement(844..1425)
                     /locus_tag="VGR03_10775"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEV1994806.1"
                     /translation="MNPTRRDFFKLTTIGGAAAAVFGFDLKPAYAQLRTLKIARANET
                     RSTCPYCSVSCGVIIYTIGDRAKNVTPQVVHVEGDPDHPINRGTLCPKGASLEQDILN
                     ERRFVKPQVRRPGGTDWEDISWDNAIAEIAQRVKKTRDETFIEKDAAGHTVNRLESIA
                     WTGGCTDTNEFNYLVVKSMRALGVVHLENQARV"
     gene            complement(1584..2906)
                     /locus_tag="VGR03_10780"
     CDS             complement(1584..2906)
                     /locus_tag="VGR03_10780"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEV1994807.1"
                     /translation="MSAQTPQDQYRKKLTTASTAVESIRNGSILCIALGMGMPAGLAR
                     AFADRVLAGNLKDLILYYQHSMKYAGETFARPEVLEKVNARNFFIGEPDRKVIDQGIS
                     DGRKYLSYIPCNFSQIPRALTEHVHVNTFLVTVSPMDASGHFSLGTNNDYASTVIRKC
                     DHVIVEVNKNMPRVFGDSPVHISEVHAIVENHVPLPEIPSKPPDEDSLTTGKFIAAQI
                     PDGATLQLGIGNLPNAVAHHLAHRNDLGIHSELFSPAFVGLIRSGVINGQKKTLHPRK
                     HVFTIAVGDANMYAFMNDNPAIESYSSAYVNDIRIIAQHDRLISVNTAIEVDLYGQVN
                     AEFINGHQYSGSGGQFDFVKGAAFSKGGNSFIALKSAAKKATISCIVPRVQMATDTRM
                     DVEHIVTEYGCVNLRGKSTKERAELLMSIAHPNFREQLADHAKSINLI"
     gene            3254..4510
                     /locus_tag="VGR03_10785"
     CDS             3254..4510
                     /locus_tag="VGR03_10785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV1994808.1"
                     /translation="MATPPNSPKHDLGSLRIGDGQRKGSGMGRRVIYAAIPVFIFAGI
                     VAAAYAFRSQKPVVEVATAAKPEAGGRQALLNASGYITPRRRATIAAKITGRVTGVFF
                     DEGTRVAQGQLLATLDDSDVKRSLDSAKADRDSAQAGIADFEVQLKNAQIGLHRAEQL
                     QKAGVQTQEALDNAGTAADSLRAKITLAKQQVAASEARIGVAQQAVDNCTIRAPFAGV
                     VVSKDAQVGEMVSPNSAGGGFTRTGIATIVDMHSNEIEVDVNESYIARVENGQQVTAT
                     LDAYPDKPFPAKVRTVIPTADRQKATVKVRITIIDLEKYNFILPDMGVKVAFLENEQP
                     AAKDKTKDKGPQAVAFIPKSAVRSDSSASFVYLVRDGKIERHAVSLGMDRGTDVAILA
                     GVSPGDSLVVKGPESLHDGDKIEIRQ"
     gene            4520..5263
                     /locus_tag="VGR03_10790"
     CDS             4520..5263
                     /locus_tag="VGR03_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1994809.1"
                     /translation="MIRVDGNGDSSSLIRVRGLNKTYSRGGEKIQVLQGLNLDVDKGD
                     FVAFMGPSGSGKTTLLNLLGGLDVPSAGGITVDGDEITHMSASKLTQWRARHVGFIFQ
                     MYNLIPVLTAFQNVELPLLLTKLSKAERRKHVETALGVVNLTDRMNHYPRQLSGGQEQ
                     RVTIARAIVSDPTFLLCDEPTGDLDRKSADEIMALIDELVGKYNKTILMVTHDPVVAA
                     RAHTTLHLEKGVLIEGGKSKQAVLAGGAA"
     gene            5260..6408
                     /locus_tag="VGR03_10795"
     CDS             5260..6408
                     /locus_tag="VGR03_10795"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1994810.1"
                     /translation="MKFSRLILANLFRKKVRLILTIGSFAIALVLFTFLAVVKSAFSR
                     GTEIAGADRLVVVSRIGLMQLMPISHRDKILAIPGVKAVTHNHWFGGVYQDEKNFFPQ
                     FVIDPEYQRQVMTELVVPEDQWNNFLKDRQGAIAGARTAARFHWKVGDRIPIKTTLYG
                     GAAWEFNLDGIYHSDRPGGDESQFWLQWKYFDENALAAVKSTAGWYVVKLDSPDDAVR
                     VAKAIDEKFGNSSFETKTETESAFAAGFVKQFGNIELLILTIGSVVFFTLLLVTGNTM
                     AISVRERTSELAVLKAIGFSGRAVLFFVLAESIVIALAGGLLGLTLAYLAIPVVGVAL
                     TGLLPPLILSKSLLTLGLVFALFVGAASGLLPGIGAMRMRVVNALRRA"
     gene            6410..7579
                     /locus_tag="VGR03_10800"
     CDS             6410..7579
                     /locus_tag="VGR03_10800"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1994811.1"
                     /translation="MAIPLVYNVRSVKARWTSTIVAVVGIAGTVGVFVAMLSLARGFR
                     ATLVSSGSADNALITRAGATSEMTSGVSLDSVKIIQDAPGIARGADGPLLTPEAVLMA
                     PIPLLSTGTDANVVFRGVAPNVLAIRGKVKIVEGRMFRPGLTEIVVGKNAKATYSGLT
                     LGSAIGLGSARWQVVGIFDAGGSAFDSEVWGDAHLLNGAYKRPDTFSQSVTVHLASPA
                     ALQQLRDTLSADPRLNVDVTREIDYYSKQSTRMTQLITTLGGFVAAIMAIGAVFGALN
                     TMYSAVAERGREIATMRALGFSSGNVILSFLVEALLISFVGGLLGCLAVLPLNGLTTS
                     TMNFQTFSNLAFAFKITFDLLWMGVLFALVMGVLGGMPPAIRAASRPVATALREL"
     gene            complement(7656..7997)
                     /locus_tag="VGR03_10805"
     CDS             complement(7656..7997)
                     /locus_tag="VGR03_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994812.1"
                     /translation="MAQEKKVAQKNGVDISKMGAYRALAQHMYADFQKGDLAAAAELG
                     TILERLWDKAEEYGGDTALAKTNRQLFDEIDKAMDQFITPITEAKGKPPDPAKVKAAY
                     NLYLEKLKLAD"
BASE COUNT         1622 a         2544 c         2380 g         1868 t
ORIGIN      
        1 attcacgccg ccgcccgcgc gccccacgtt gcccagcaga agttgcagga tcgccgccgt
       61 tcgaatgatc tgcgtcccga aagtgtggtg cgtccatccc acggcgtaga tgatggtcgc
      121 cgcttttttc atgtcgccgt ctttgcgcac cgacgtaaag agttctgctg ccttctcgaa
      181 ttgatctctt ggaatccccg taatccgttc caccatctcc ggtgtgtagc ggctgtagtg
      241 ttttttcaga agctggaaaa cgcaattcgg attctgcagc gagaagtcgt aggcgacttt
      301 ttccggcaag gccggcggtg gaggcggcgc cccgtttgct ggatgcgccg cagccgcaac
      361 agcatgcgcc tccggcatgg gcttgccgct ggcgtctcca cccgcgtaat tccagctcga
      421 cttctcgtag gtttgggact tggcgtcgaa gccggagaaa acgccgtccg tttccggcag
      481 tttgaagcca tcctttacga taaaggcggc gttggtgtaa ttgagcagat agtctttggc
      541 aatgcggttg ttctcgatcg cgtagcgaat cactccgccc agaaacgcga tgtccgcccc
      601 ggcgcgaatc tggcagaaga gatcggccac cgacgccgtc cgcgtaaagc gcggatccac
      661 cacgatcatt tttgcgttgc gattgcgttt cgcctcgatg gcccacttga atccgcaagg
      721 atgattctcg gccggattgc cgcccatgat caacatcatg tcggtattct tgatgtccac
      781 ccaaccattg gtcatcgcgc cccggccaaa cgttggtccc aaacttgaga ccgtggggcc
      841 gtgtcaaact ctggcctggt tttccaggtg cacgaccccc agggcgcgca tcgacttcac
      901 aaccagataa ttgaactcat tggtgtccgt gcaaccgccg gtccaggcga tcgattccag
      961 ccggttaacg gtgtggcccg cggcatcctt ctcgataaaa gtttcgtccc gcgtcttctt
     1021 cacacgctgg gcgatttccg caatggcatt gtcccaggaa atatcctccc aatctgttcc
     1081 gcccgggcgt cgcacctgag gtttcacgaa acgccgttcg ttcaaaatat cctgttcgag
     1141 ggacgctccc ttggggcaga gcgttccgcg attaatcgga tgatcaggat cgccctcgac
     1201 gtgcaccacc tgtggcgtga cgttcttcgc gcggtccccg atggtgtaaa taatcacgcc
     1261 gcagctcacc gagcagtacg gacacgtcga gcgtgtctca ttggcccgcg cgatcttcag
     1321 cgtccggagt tgcgcatacg cgggcttcaa atcgaagcca aacaccgcag ctgcggctcc
     1381 accaatggtc gtcaacttaa agaaatcacg gcgggtaggg ttcatgccgg gcccccatcc
     1441 gcgagcagtg aagtaaggta gggagcgtct cgcggaagtt acttaataga acttgccagc
     1501 ccatgtgtca aggacgattt ttgagaatcc ggaacgtccc cccgccgcaa agttggcggt
     1561 atccaggggc atccgcgcag catctaaatg agattgatcg attttgcatg atccgccaat
     1621 tgctcacgga agttcgggtg ggcgatgctc atcagcagtt cggcgcgctc cttggtcgat
     1681 tttcctctga ggttcacgca tccgtactcc gtcacaatgt gttccacatc catgcgcgtg
     1741 tccgtggcca tttgaacgcg aggaacgata cacgaaatcg tggctttctt ggccgcggac
     1801 ttgagggcga taaaggagtt gccgcctttg gaaaaagctg cgcccttcac aaaatcgaat
     1861 tgtccgccgg agccgctgta ctggtgcccg ttgatgaact ccgcgttcac ctgcccgtag
     1921 aggtcaacct cgatcgcagt gttcacgctg atcagccggt cgtgctgggc gatgatgcgg
     1981 atgtcgttca cgtaggcgga cgaatagctt tcaatggcgg gattgtcgtt catgaacgcg
     2041 tacatgttgg cgtcgcccac agcaatggtg aaaacgtgtt tccgcgggtg cagcgttttt
     2101 ttctggccgt taatcacccc gctgcggatc aatcccacga acgcggggct gaacagctcg
     2161 gagtgaattc ccaaatcgtt gcgatgcgcc aggtggtggg ccaccgcatt cggcagattc
     2221 ccgatgccca gttgcagcgt cgccccatca ggaatctgcg cggcgatgaa cttgccggtc
     2281 gtcaaggagt cctcgtcggg cggcttcgat gggatttccg gcagcggcac gtggttttcg
     2341 acgatggcat gcacttcgga aatatgcacg ggcgaatcac cgaacacccg gggcatgttc
     2401 ttgtttactt cgacgatgac gtggtcgcat ttgcggatca cggtcgacgc ataatcgttg
     2461 ttggtcccca ggctgaagtg cccggaggcg tccatgggcg aaacggtgac gagaaacgta
     2521 ttgacgtgca cgtgctcggt cagcgcccgc gggatctgcg agaaattgca cggaatgtag
     2581 ctcaagtatt tccggccgtc ggaaataccc tgatcgatga ccttgcgatc gggttcaccg
     2641 atgaaaaaat tgcgcgcgtt cactttctcc agcacttcgg gccgcgcaaa cgtctccccc
     2701 gcgtatttca tggagtgctg gtaatacaaa atcaaatcct ttaagttacc cgcgagaacg
     2761 cgatcggcaa aggcgcgcgc cagtcccgcc ggcatgccca tgccgagtgc gatgcaaaga
     2821 atacttccat tgcgaatcga ttccacggca gtggaagcgg tcgtcagttt cttgcggtac
     2881 tggtcctgcg gggtttgcgc tgacattagg gtcctcgttg gcgatcaata gggatgtgtt
     2941 ccgcgcgaag gatgccgcgg acaaaataga ctgaagcaga gcgcttggtt ttgcaacctg
     3001 ctgcaccagt ttgggaaaca cacgccctgg aaccgattcc gccctgtgcc tctgggtcct
     3061 ccagtccttc ggctgcacgc cttctcgacg cgcgcccgca ccaggccgcg gagggcgact
     3121 ctgctcgccc aaaaccactc cttcgcgata gaatggttgt gacctcagac ctgcctcaat
     3181 catctgttat ttttagaggc tgcaatccgt agggtagtgt gaagcttacc aatcagaagg
     3241 agcaggggcg ctaatggcga ctccaccgaa ttccccaaaa cacgacctcg gctcgttgcg
     3301 cattggtgat ggccagcgca agggttccgg catgggcagg cgcgtcatct acgcggccat
     3361 acccgtcttc atcttcgcag gcatcgtcgc ggctgcctat gctttccgca gtcagaagcc
     3421 cgtggtcgag gttgcgaccg ccgcgaagcc cgaggcgggc ggccgccagg cgctgctgaa
     3481 tgccagcggc tacatcacgc cgcgccgccg cgccaccatc gccgccaaga ttaccggccg
     3541 cgtcaccggc gtcttctttg acgaaggcac ccgcgtcgcc cagggtcagc tcctcgccac
     3601 gcttgatgat tccgacgtca agcgctccct cgattccgcc aaagccgacc gcgactccgc
     3661 ccaagccggc attgccgatt tcgaggttca gctcaagaat gcgcaaattg ggctacaccg
     3721 tgctgagcaa ctccagaaag ccggcgtcca gacgcaggag gctttggaca acgctggcac
     3781 cgccgcggat agtctgcgcg ccaaaattac gctcgccaag cagcaagtgg ctgcctccga
     3841 agcgcgcatt ggcgttgccc agcaggcggt ggacaattgc accattcggg ctccctttgc
     3901 gggcgtcgtc gtctcgaagg acgctcaggt tggcgaaatg gtctcgccca attccgcggg
     3961 tggcggcttt acccgcacgg gcattgcgac gattgtggat atgcactcca acgaaatcga
     4021 agttgacgtc aacgagtcgt acatcgcgcg cgttgaaaac ggccagcagg tcaccgcgac
     4081 tctggatgct tacccggata agccgttccc cgcaaaggtt cggaccgtca ttcctaccgc
     4141 tgaccggcaa aaggccaccg tgaaagtgcg catcaccatc atcgacttgg agaaatacaa
     4201 tttcattttg ccggacatgg gcgtgaaggt cgcttttctg gagaatgagc agcccgccgc
     4261 aaaggacaag accaaagaca aagggccgca agccgtggcg ttcattccca agagcgccgt
     4321 gcgctccgat tccagcgcct ctttcgtgta tttggtccgc gatggaaaaa tcgaacgcca
     4381 cgccgtcagc ctgggcatgg atcgtggcac ggacgtcgcg attctagcgg gtgtctctcc
     4441 tggcgactcc ctggtggtca aggggccgga aagtttgcat gatggcgaca aaattgaaat
     4501 ccgtcagtag gagctgaaca tgattcgagt tgatgggaac ggcgattcgt ccagccttat
     4561 ccgcgtgcgc ggtctgaaca agacctacag ccgcggcggc gagaaaatcc aggtcctcca
     4621 gggcctcaat ctggacgtcg acaagggtga ctttgtcgct ttcatgggcc ccagcggctc
     4681 cggcaagacc acgttgctca atctcctcgg cggtctcgac gtgccctcgg cgggcggcat
     4741 caccgtcgac ggcgacgaaa ttacgcacat gtccgcgagc aagcttacgc aatggcgcgc
     4801 ccgccacgtc ggttttattt ttcagatgta caacctcatc cccgtgctca cggcgtttca
     4861 aaacgtcgag cttccgctgc tcttgaccaa gctttccaag gcggagcgcc gcaagcacgt
     4921 ggaaaccgct ctcggtgtcg tcaatctcac cgaccgcatg aatcactatc cgcggcaact
     4981 ttccggcgga caggaacagc gcgtcaccat cgcacgggcc atcgtctccg acccgacgtt
     5041 tctgctgtgc gacgagccca ccggcgacct ggaccgtaag agcgcggatg aaatcatggc
     5101 gctgattgac gagctggtcg gcaagtacaa caaaacgatt ctgatggtca cgcacgaccc
     5161 ggttgttgcc gcgcgcgcac acacgacact gcatctggag aagggcgtgc tcatcgaggg
     5221 tggcaaatcc aaacaggcgg ttctggccgg aggtgcggca tgaaattcag ccgcttgatc
     5281 ctcgccaatc tgttccgcaa aaaggttcgc ctcatcctca ccatcggctc ttttgccatc
     5341 gctctggttc tcttcacgtt tcttgccgtg gtgaagagcg ccttcagccg cggcacggaa
     5401 atcgcgggcg cggaccgcct ggtggtcgtc agccgcatcg gtctgatgca actaatgccg
     5461 atctcccacc gcgacaaaat cctggccatt cccggcgtca aggcggtcac gcacaatcac
     5521 tggttcggcg gcgtctatca ggatgaaaaa aacttctttc cacagtttgt catcgacccc
     5581 gaatatcaac gccaggtgat gactgagctt gtggtgcctg aagaccagtg gaacaatttc
     5641 ctgaaagacc gccagggcgc catcgcgggc gcccgcacgg ccgcacgctt ccattggaaa
     5701 gttggcgatc gcatcccgat caaaacgaca ctctacgggg gcgccgcttg ggagttcaat
     5761 ctcgacggca tttatcacag cgatcgtccc ggcggcgacg agagccagtt ttggctgcag
     5821 tggaaatact ttgacgagaa tgcgctcgct gccgtcaaga gcacggccgg atggtacgtg
     5881 gtcaagctcg atagccccga tgacgccgtg cgcgtcgcca aggccattga cgaaaagttt
     5941 ggcaactcgt cgtttgagac caagaccgag acggaatccg ccttcgccgc aggtttcgtc
     6001 aagcagttcg gaaacatcga actcttaatc ctcactatcg gcagcgtcgt cttctttacg
     6061 ctactcctgg tcaccggcaa cactatggct atctctgtgc gcgagcgcac ctcagaactg
     6121 gccgtcctaa aagccatcgg cttttccggg cgcgctgtcc ttttcttcgt cctggcagag
     6181 tccatcgtca tcgctcttgc tggcggctta ctgggcttga cgctcgccta cttggccatt
     6241 cctgttgtgg gcgtggctct caccggcctt ctgccccctc ttatcctttc caagtcgctt
     6301 ctgacacttg gacttgtttt cgccctcttt gttggcgctg ccagcggcct gctgccgggg
     6361 atcggcgcca tgcgtatgcg cgtcgtgaac gcgttgagga gagcctgaaa tggcgattcc
     6421 actcgtttac aacgttcgga gcgtcaaagc tcgctggacc tccacaatcg ttgcggtggt
     6481 ggggattgcc ggaaccgtgg gtgtcttcgt agccatgttg tcgctcgcgc gcggattcag
     6541 ggccacactt gtttcttccg gctccgcgga caacgcgctc attacccgcg cgggggccac
     6601 atccgaaatg accagtggag tctctctcga ttcggtcaaa attatccagg atgctccagg
     6661 catcgctcgt ggggccgacg gcccgttgct gacaccggag gctgtcctga tggcgccgat
     6721 tccgttgctt tccaccggca cggatgccaa cgttgtgttt cgtggcgttg cgcctaacgt
     6781 ccttgccatt cgcggcaagg tcaagatcgt cgagggccgc atgttccggc ccggtctaac
     6841 cgaaatcgtc gtcggcaaaa acgccaaggc gacctattcg ggattgactc tgggaagcgc
     6901 aattgggctc ggtagcgcgc gttggcaagt ggtgggcatc tttgacgccg gcggaagcgc
     6961 cttcgattcc gaggtttggg gcgacgccca cctcctgaac ggtgcgtata agcgtcccga
     7021 cactttttct cagtccgtta ctgtgcacct cgcttcgcct gctgcccttc agcaattgag
     7081 ggacactctc agcgccgatc cgcgtttgaa tgtagacgta acccgcgaaa tcgactacta
     7141 ctccaagcag tccacgcgca tgacccagct catcaccaca ctgggcgggt tcgtcgcggc
     7201 gatcatggcc atcggcgcgg tctttggcgc cctcaacacc atgtactccg ccgtcgccga
     7261 acgcggccgc gaaattgcca ccatgcgcgc gctcggtttc agctccggga acgtcatcct
     7321 ctcctttctc gttgaagctc ttttgatttc ttttgttggc ggcctccttg gctgcctcgc
     7381 cgtcctgccc ctcaacggct tgaccaccag caccatgaat tttcagactt tttccaacct
     7441 ggccttcgcc ttcaagatca cctttgatct tctttggatg ggcgtgctct tcgctctggt
     7501 catgggtgtc ctcggcggca tgcccccggc gattcgcgcc gcgagtcgtc ccgtggccac
     7561 agccctccgc gaactgtaga ctcggaggga aactcattag cgaggcgctt acggcgcgcc
     7621 gaaagcgctt cgttttctaa gctcaaccgg aagggctaat ccgccagctt caatttttcc
     7681 aggtagaggt tataggcggc cttcaccttg gcaggatcag gcggcttgcc tttcgcttcc
     7741 gtgattggcg tgatgaactg atccatggct ttgtcgattt cgtcgaagag ttggcggttg
     7801 gtcttcgcca gcgcggtatc gcctccgtac tcttccgctt tgtcccaaag tctttccagt
     7861 atcgtcccca gttccgcggc tgctgccaag tcgccctttt ggaagtcggc gtacatgtgt
     7921 tgtgccaggg cgcggtaggc tcccattttt gagatatcca cgccattctt ttgcgcgacc
     7981 ttcttctctt gcgccaaagt cgatccacct gcagcaatgg ccagcaagaa tacgaagaac
     8041 aggaaggaaa ggggtctgct cgctctatgg aatgggaaat tcattgcagc tccttcattc
     8101 gcgcgagatt ctgccccacc agctggtgaa tccgcgtgaa cttcagtctc ttacagtttt
     8161 gtagcacgaa tcgaaagaat gcgaaccgtg ccggttcgcc catctgtgag caaaaaggta
     8221 cgcataccct ggcgcgtttt ggagaagcct aatccgtgcc tcgggattct tcgcgctggc
     8281 gtggggcggc cgcaaactcg ccgtggagtg gaaaccatgt ccaactcttt aacgccattt
     8341 tcgaattggt gttctattct ttggcatgat ggcccttgac gattccatga ctgcgaatct
     8401 agcctaggtg ggag
//
LOCUS       DASYVO010000319         4066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_122365, whole genome shotgun sequence.
ACCESSION   DASYVO010000319 DASYVO010000000
VERSION     DASYVO010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122365"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="VGR03_10810"
     CDS             complement(<1..1214)
                     /locus_tag="VGR03_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994813.1"
                     /translation="MTTAGSFDAPIEIFGGLVTDMSPADLPHGVSPDCQDVIFSNGGV
                     ATRPGLQALFGPLAGNPTVNYVKTYETLNATLRTMALDSNGVLYKETTPGALSSIANG
                     LAANAYANSTTLFGREYLAISDGKTGNDLPRQYDDTNFDRVSQGGPGAGPTVVDENAI
                     VAITASPNGATQPAAVTIVASPNGATENGFLVTITTTAAHGLSAGQSVTIAGVGVAGY
                     NGTFPVVSAPTTTQFTYIAGASGLAASGGGTAASATATIQSTAAHGFVVGQLVTTTGI
                     GVAGYNGTFPIAAVPDSTHFAFTAAAGGLAASGGGTAAAAGSVSPGVHQVCVIFKTRQ
                     GYLTKPGPATSWTASGGKRAVVTNIPTGPSNVVGRILCFTGAGGASFFYTGSGSTLFS
                     GNMVIGDNTTTS"
     gene            complement(1234..1881)
                     /locus_tag="VGR03_10815"
     CDS             complement(1234..1881)
                     /locus_tag="VGR03_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994814.1"
                     /translation="MSTETSTAVLVNISTEKWPPRHRTYFGSLEVRSPQPGEPYALTP
                     IRGCTGVMDLGDKRTMEYRITAKEIADDIAREINGDSGEGSFHGVFVAAGPEPTEAEL
                     ADARRKLEEFQRHLVAAADLEWERTKNPMFITDLERRAARQLGQEKPWLYDPKPLAEC
                     PVCAEKIKHGVAVCRSCGAILDREKAAQYGLVGKEGKTATEPQKHREKREQEAGK"
     gene            2051..3616
                     /locus_tag="VGR03_10820"
     CDS             2051..3616
                     /locus_tag="VGR03_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994815.1"
                     /translation="MKTSALLARIVSAAILLFAFGVAVYRAKVLPIAHDEALTYEWFL
                     DQGVYDVLRYNPANHVLQTLLAKPIVKILGVSEFTLRIPTLFGAAMYLITVYFLCRKL
                     FGDGLVLVLSTAMMALNPQVMDFMAAARGYSLGLAGLAVAMYAFARLANRGKFDPADK
                     EWRWGCAAASISLALAVAANFTNIVPAACLALCFTVSVLGGLASVFRWRDGALREFAK
                     YFIFPGVAVGFCVLWPFLIQARLAQTKIQLDKASDAARDIFNASFLYKWTEDVFNSLG
                     AVPSSAGSWQAGVTDLGANFLLPLLFCFVLIGLTLACRTPADLGKHQNAQCRIFAGAA
                     IASVVLTVVLHFVTKVNYPFSRYCLFVVPLFTIGGVLAARQISSRIPSLLLKSAGLVL
                     AAIVVSDYALAINVKSFRYNAYDIISRDLYQAIEKDALSRGLTNVRVGGTWWYEPEIN
                     FYRLHHRAKWMLPYEIKDCSYWWQTPGALEPAAYDYFVFVPPSDPQLTGPRVRTIFHD
                     EKTQATIIAIAHD"
     gene            complement(3643..>4066)
                     /locus_tag="VGR03_10825"
     CDS             complement(3643..>4066)
                     /locus_tag="VGR03_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994816.1"
                     /translation="LAQLDTEQDWTKTQHFTQGVALGTETLTASPRSEQNLFLPGALT
                     STWTGATWTNDKAVTITRVQVQAKTAPAGCTTNAIVRFTDGTTPVNVTISAAANDSGA
                     ISQNYAAGSSLQVLVQTAAAGCTTSPADANVTVQYRMQ"
BASE COUNT          691 a         1203 c         1233 g          939 t
ORIGIN      
        1 atggaagtcg tggtgttgtc gccgatgacc atgtttccgc tgaacagtgt tgagccactg
       61 ccggtgtaga aaaagctcgc tccgcccgcg ccggtgaagc acagaattct gccgacgacg
      121 ttcgatggcc ctgtcggaat gttggtgacc acggcgcgct tgccaccgct ggcggtccag
      181 cttgttgccg gaccgggttt cgtgaggtac ccctgtcgcg ttttgaaaat cacgcagact
      241 tgatgcacgc ccggcgaaac gcttccggct gcggccgcag ttccaccgcc ggaagcggcg
      301 aggccgcccg cggctgctgt gaacgcaaag tgcgtggaat ctggcacagc ggcgattgga
      361 aatgtgccgt tgtaaccggc cacgccgatg ccagtggtgg tcacaagctg tccgacgaca
      421 aatccgtgcg cggcggtgct ctggatcgtg gcggttgcgg aggccgccgt gcctccgccc
      481 gaagcggcga gtcccgaagc gccggcgatg tacgtaaact gtgtggtggt tggcgcggag
      541 accacaggaa acgtgccgtt gtagccggcg acgccgacac ccgcgatggt caccgattgt
      601 cctgccgaaa ggccgtgcgc ggcagtcgtg gtgatcgtta cgagaaaacc gttttcggtg
      661 gcgccgttgg ggctggccac gatcgtgaca gcagcgggct gcgtggcgcc gttggggcta
      721 gcagtgatcg caacgattgc gttttcatcg acgacggttg gtcccgcgcc cgggccgccc
      781 tggctgacgc ggtcgaaatt cgtgtcgtcg tactggcgtg gcaggtcgtt gccggttttt
      841 ccgtcgctga tggccaggta ttccctgcca aaaagcgtcg tcgaattcgc gtaggcattg
      901 gccgcgagac cgttggcgat gctcgaaagc gcgccgggag tcgtttcctt gtagagcacg
      961 ccattggagt cgagcgccat ggtgcgcaag gtggcgttca atgtttcgta ggtcttgacg
     1021 tagttaaccg tcggattgcc ggcgagcggc ccgaaaagcg cttgcaatcc cggccgcgtc
     1081 gccacgcctc cgttgctgaa gatcacgtcc tggcaatcgg gcgacacgcc gtgcggcaaa
     1141 tccgccggag acatatcggt caccagcccg ccaaaaatct cgatgggcgc gtcaaatgaa
     1201 cctgcagttg tcatactctc attttcttaa ctcttacttc cctgcttcct gctcgcgctt
     1261 ctctcggtgt ttctgtggct ccgtggcagt ctttccttcc ttgccgacca agccatattg
     1321 cgcggctttt tcgcggtcga ggatggcgcc gcaggagcgg cacaccgcca cgccatgttt
     1381 gatcttttcc gcgcagacgg gacactccgc cagcggtttg ggatcgtaga gccacggctt
     1441 ttcctgaccg agctgcctcg cggcgcggcg ctcgaggtcg gtgatgaaca tgggattctt
     1501 ggtgcgctcc cattcgagat cggcggcagc cacgaggtgg cgctgaaatt cctcgagctt
     1561 gcgacgggcg tcagcaagct ccgcttcagt aggctcgggg cctgcagcga cgaagacgcc
     1621 gtggaaactt ccctcgcccg aatcgccgtt gatttcccgc gcgatgtcgt cggcgatttc
     1681 cttcgcggtg atgcggtact ccatggtgcg cttgtcgccc agatccatca cgccggtgca
     1741 tccgcgaatc ggagtgaggg cgtacggttc gccgggctgc ggcgaacgca cctccagcga
     1801 accaaagtag gtacggtgcc gcggcggcca cttttccgtg gaaatattga ccaacacagc
     1861 tgtgctcgtc tccgtgctca taaacgcctc actttttctg aaacattcgg gcctactcag
     1921 cccctaccca ctcgcggtat cattccgtcc ggtcttgctt gggagggact atttcaattc
     1981 ctcagttcgc aactggaaac cagcgcagcc gccgcggcgg gcgtcatgcc ggcgacggca
     2041 aacaatccac atgaaaacca gcgctctgct tgcgcgaatc gtgtcggccg cgatattgct
     2101 ttttgccttt ggcgtagcgg tgtatcgcgc caaggtattg cccatcgcgc acgacgaggc
     2161 attgacctac gaatggttcc tcgaccaggg tgtttacgac gtgttgcgct acaaccccgc
     2221 caatcacgtc ctgcagacgc tgctagcaaa accgattgtg aaaatactcg gtgtttccga
     2281 attcacactt cgtattccaa ccctcttcgg cgccgccatg tatctgatca ccgtgtactt
     2341 cctctgccgg aagctttttg gcgatggcct ggtgctggta ctctccacgg cgatgatggc
     2401 tttgaatccg caggtcatgg acttcatggc ggcggcccgc ggctacagtc ttggcttagc
     2461 aggtctggcg gtggcaatgt acgcattcgc gcggcttgct aatcgcggga aatttgatcc
     2521 cgcggacaaa gagtggcgct ggggatgcgc ggccgcttcc atttccctgg cccttgccgt
     2581 ggcggcaaat tttacaaata ttgttcccgc ggcttgcctg gcgctttgct ttactgtttc
     2641 ggtattgggc ggactcgcta gtgttttcag atggcgggac ggtgcgcttc gcgagtttgc
     2701 aaagtatttc atttttcccg gtgttgctgt cggcttctgt gttctctggc cgttcttgat
     2761 tcaggcgcgc ctcgcgcaga ccaaaattca attggacaaa gcgtccgatg ccgcacgaga
     2821 catattcaac gcttcgtttc tctacaagtg gacggaggat gtcttcaaca gtctgggcgc
     2881 ggtgccatct tccgcaggaa gctggcaagc gggagtcacg gatctgggcg ccaatttcct
     2941 cttgccgctg ctcttctgtt ttgtgctgat tggcttgact ctggcatgcc gcaccccggc
     3001 cgatttaggg aagcatcaaa acgcacagtg ccgaattttc gcaggtgcgg cgatcgcgag
     3061 cgtagtttta accgtcgttc ttcacttcgt gaccaaggtc aactatccct tcagccggta
     3121 ctgcctcttc gtggttccgt tgtttacgat cggcggagtg ctagctgctc gacagatttc
     3181 gtctcgaatt ccttcgttat tgttaaagag cgccggtttg gtccttgccg caatcgttgt
     3241 ctctgactac gcgttggcaa tcaacgtgaa atccttccgc tacaacgctt acgacatcat
     3301 ctctcgcgat ctttatcaag cgattgagaa ggatgcgctt tcgcggggcc tgaccaacgt
     3361 gcgcgtgggc ggcacctggt ggtacgagcc ggaaatcaat ttttaccggc tgcatcaccg
     3421 cgccaaatgg atgctgccct acgaaatcaa agactgctcc tattggtggc aaacgcctgg
     3481 cgcgctcgag cccgccgcct acgactactt tgttttcgtt ccaccgagcg acccgcaatt
     3541 gacgggcccg cgcgtccgga ccatcttcca cgacgagaag acgcaagcga cgatcatcgc
     3601 gatcgcgcac gactgaacgc agctcgcttc gcttttcgat cgctactgca tgcggtattg
     3661 gacggtgacg ttggcgtccg ccgggctggt ggtgcacccg gctgccgccg tttggaccag
     3721 gacttgcagc gatgagcctg ccgcgtagtt ctgcgaaatc gcgccgctgt cattggctgc
     3781 cgcggaaatc gtgacattga ctggtgtcgt gccgtcggtg aaccggacaa tcgcgttggt
     3841 tgtgcaacca gcgggcgccg tcttcgcttg cacttgaacg cgcgtgatgg tgacggcttt
     3901 atcgtttgtc caggtcgcac cggtccaggt ggacgtcagc gcgccgggca ggaatagatt
     3961 ctgttccgag cgcgggctgg cggtgagggt ttccgttccg agggccacgc cctgcgtgaa
     4021 atgctgcgtc ttcgtccagt cctgctcggt gtcgagctgc gcgagc
//
LOCUS       DASYVO010000320         5865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_62956, whole genome shotgun sequence.
ACCESSION   DASYVO010000320 DASYVO010000000
VERSION     DASYVO010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62956"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VGR03_10830"
     CDS             complement(<1..556)
                     /locus_tag="VGR03_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1994817.1"
                     /translation="MIHVSAAWRDAVLLLAAAPLVYYSLATLAALRFFKRERARILPN
                     YTPSVSVLKPVRGVDFGSHENFASFCRQEYPYYEILFAVNDDADPAVPVIRRIIAEFP
                     ERKIRLLVGAEHLGTNRKVNKLARLAREAQNEVLVLTDGDVRVGPHFLHEVVAPLADN
                     KIGAVTCFYRGIAESNLGAEIEAVG"
     gene            complement(711..1895)
                     /locus_tag="VGR03_10835"
     CDS             complement(711..1895)
                     /locus_tag="VGR03_10835"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1994818.1"
                     /translation="MLKIFLIFGLFGILTSTIYLALVFAATVRFRRRPRTVPAFTPPV
                     SVLKPLHGAEPGLREFLEGFFQLDYPEYEILFCARHETDPGLAIARDLSEKYPRVPVR
                     IMTSGEPPWPNARCFSLNEMKSVARHDILVITDSDVRVKPGYLRAVVEPFREERTGLV
                     TCVYRGVAAQGGFWARLEGLGMSVEMTSGVLVAEMLEGMRFALGPSMVVRKSCVDQIG
                     GFEKLGDYYADDFMLGKLVAGSGSTVVLSDHVIDHCILNTKFEKSLGHQWNWMKSTRF
                     SRPMGHLGTGLTFGLPFGLLVFLAATMIGKPWLGLAALGWTVAARVFQSVLVGWYVVK
                     DKDAVRLAWLYPLRDLMGSLLWMASYASRRVGWRDDFFELTKRGVVRLIPAEERRAGA
                     QH"
     gene            complement(2300..2671)
                     /locus_tag="VGR03_10840"
     CDS             complement(2300..2671)
                     /locus_tag="VGR03_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994819.1"
                     /translation="MMRVILALAVLILGSTGGEIAITHGMKATGEPARLRPLALLQFL
                     GRAVRNGWFWAGVPLMALSFYSLLVLLSWEPISLVIPASALSYVVGTLGAKYLLGEQV
                     SAARWAGVVLVCAGVALVAAG"
     gene            complement(2668..3096)
                     /locus_tag="VGR03_10845"
     CDS             complement(2668..3096)
                     /locus_tag="VGR03_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994820.1"
                     /translation="MASERRLRIKTLVMVLAMVVCANAGDLMLKRGMSQIGAVQLTAL
                     GLAQAFRLTVTNGTIWVGILFLTGFMLSYMTVLSWADYSYVMPAGAFGYALLTLLAVI
                     FLHESVSPRRWVGVVLICAGVLLVGQTKPRTTAVAGEAVA"
     gene            complement(3107..4609)
                     /gene="hpnJ"
                     /locus_tag="VGR03_10850"
     CDS             complement(3107..4609)
                     /gene="hpnJ"
                     /locus_tag="VGR03_10850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HEV1994821.1"
                     /translation="MPDVMKTLLLNPPSFENFDGGASSRWPATREIESYWYPVWLTYP
                     AGMIPGSRLLDASPHKVNWQQTIQICKDYEFLILFTSTVGLASDVKLIRKIKEANPGM
                     KIAFVGPTGHIKPDETLNASADIDFVTRGEFDHSVVEYAHGKPLSEILGVSYRKDGKI
                     VHNVERPQLHTEELDALPFATDIYKRDLEIERYNVPFLLHPYVSFYTTRGCPALCTFC
                     MWPQTISGHAWRTRSTENVAREVKQTLEHFPQMKEIFFDDDTFNIRKDRVLDLCAKFK
                     PLKFQWSSTARVHSDYDTVKAMADGGARLFIVGFESGDAQILKNIKKGATVEMGRAFM
                     KNCRKVGIKVHGDFIIGLPGETKDTIQKTIEFAKELDCETVQVSLAHAMPGTELHDTM
                     AQQGFLKIEALADSGGHQLPHIEYPHLSKVEMMSGVNRFYDEYYFRPKVVWRIVKEAL
                     WDSHERKRLYHEATEFLKLRAERLKWARKGGDFEKPMVSVPGTSSASGND"
     gene            complement(4844..>5865)
                     /locus_tag="VGR03_10855"
     CDS             complement(4844..>5865)
                     /locus_tag="VGR03_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV1994822.1"
                     /translation="LGHLCGGGTMANLEALWVAGQLQPGKKILASEQAHYTHGRISSV
                     LQLPFETVPCDARGRMDVNALAKRVERGDVGIVVATMGTTATGSVDPLPEILALRTKH
                     GFRIHADAAYGGYFALTESLGEDAKRAFARIGQADSIVIDPHKHGLQPYGCGCVLFRD
                     PEVGRLYKHDSPYTYFSSAELHLGEISLECSRPGAAAAALWATQRLLPLKKGGEFARG
                     LERGRKAALALFEKVRADARFVTPFAPELDIVVFAPNARSVSEASVLSRKIFEAAAKR
                     DLHLAVAELPIRFWEANLGEMRRDRETLTCLRSVLMKPEHLDWVTRIWDLLSLSADEI
                     LVARN"
BASE COUNT         1251 a         1881 c         1545 g         1188 t
ORIGIN      
        1 cgccaacggc ttcgatttcc gcgccgaggt tgctctcggc gatgccgcgg tagaaacatg
       61 tgactgcgcc gattttgtta tcggcaagcg gggccacgac ttcatgcaag aaatggggtc
      121 ccacacgcac gtcgccgtcg gtcaacacca gcacttcgtt ctgcgcttcc cgcgcgagcc
      181 gcgccagctt gttcactttg cggtttgtgc ccagatgctc cgcgcccacg agcaagcgga
      241 ttttccgctc gggaaattcc gcgatgattc ggcgaatcac cggcaccgct gggtctgcgt
      301 cgtcgttcac cgcaaacaaa atctcgtagt acgggtattc ctggcggcaa aaacttgcga
      361 aattttcgtg agagccaaag tccacgccac gcaccggttt gagcacgcta acggaaggag
      421 tgtagttcgg caggattcga gcgcgctccc gtttgaagaa ccgcagcgcc gccaaagttg
      481 cgagcgagta gtaaacgagt ggagccgccg ccagcagcag cactgcatcc cgccacgctg
      541 cggaaacgtg aatcatgcgg atttgcgagt catcggcggc agtttttccg ctacgcttgc
      601 tcttcaggct actctagcag cttgcgccgc acgcgccaat cctgcgcgat tttggaattc
      661 ccggatttct tgaggaacgg ctccggcagg agcatagaaa cttttggcgc ttagtgctgc
      721 gccccggcgc ggcgttcctc ggcaggaatc aaccgcacca caccgcgctt cgtcaattcg
      781 aagaagtcat cgcgccagcc gaccctgcgg ctggcatagc tggccatcca caggagcgat
      841 cccatcaagt cgcgcagggg ataaagccat gcgagccgaa cggcgtcctt gtctttcacc
      901 acgtaccagc ccaccagcac ggattgaaag acccgcgccg ccacggtcca tcccaaagcc
      961 gcaagaccga gccagggctt gccgatcatc gttgcagcca ggaacaccag taaaccgaac
     1021 ggcaatccaa acgtaagccc cgtgccgaga tgacccatgg gccgtgagaa tcgcgtgctc
     1081 ttcatccagt tccactgatg tcccagggac ttctcaaact tcgtattcag gatacaatga
     1141 tcgatcacgt ggtccgacaa aacgacggtg ctcccacttc cggcgacgag cttgccgagc
     1201 atgaagtcat ccgcgtaata gtctcctagc ttctcgaagc cgccgatctg atctacacaa
     1261 cttttccgca cgaccatgga cggacctaga gcgaatcgca tgccctccag catttcggcc
     1321 accagcacgc cggaagtcat ttccacggac atgcctagcc cttcgagtcg cgcccaaaat
     1381 ccaccttgcg cggctacgcc gcgatagacg caggtgacca gtccggtcct ctcctcccgg
     1441 aacggttcca ccaccgcgcg caaatatccc ggcttgacgc gcacgtcact atccgtaatc
     1501 accaagatgt cgtgccgtgc caccgatttc atctcattca gcgaaaagca ccgcgcattg
     1561 ggccacggcg gctctccgga agtcattatc cgcaccggca cgcggggata tttttcagac
     1621 aaatcgcgcg cgatcgccag ccccgggtcg gtctcgtgcc gcgcgcagaa caaaatttcg
     1681 tattccggat aatccagttg gaagaatcct tccaggaatt cccgcaggcc cggctccgcg
     1741 ccgtgcaacg gcttcagcac gctcacagga ggcgtaaacg ccggcacggt cctgggccgc
     1801 cgccggaaac gaaccgtggc ggcgaacacc aaggcgaggt aaatcgtcga ggtcaggatt
     1861 ccaaataacc cgaaaataag aaagattttc aacatgacaa ggcacgccgg caccgcgccg
     1921 cgctatccgt tcgcggtgca acaatgaatt cttgatgaag tccgaaaccg gatgagcgga
     1981 gggaatttgg aaaacacaaa aacgaaaaca cgcacgcagc cttccatgcg gccccaaaca
     2041 tcaacgcaac ccagccgaga gtttccggca acattccttt gccccaagtc cccatggcat
     2101 ggcgaaaatt tatgcgatgg gatcctgtcc gtcacgcagg aattctgatc tacaaactaa
     2161 cttgccgagt cagatgccaa cctgttttga ttggttgccg cggaccaacc tagaaccccg
     2221 cggcggcgcc aatccgttcc ctagaagcta tcactacttt caaatctttg gatgaatatt
     2281 taatcaggaa gccgggtgct caacccgccg cgaccagcgc gactcccgcg caaaccagca
     2341 cgacgcccgc ccaccgcgcg gcgctcactt gttcgcccag gaggtacttg gcgccaagcg
     2401 tgccgaccac gtagctgagc gccgatgcgg gaatcaccaa actgatcggc tcccaggaaa
     2461 gcaataccag cagcgaataa aaggacaggg ccatcagcgg cacgcccgcc cagaaccatc
     2521 cattgcgcac ggcccgcccc aggaactgaa gtaacgccag cggccgcaat cgggccggct
     2581 caccggtcgc cttcattccg tgcgtgatgg cgatctcgcc tccggtgctg ccaagaatca
     2641 gaacggcaag tgcaagaatg acgcgcatca tgccactgcc tcaccggcaa ctgcggtcgt
     2701 ccgcggtttc gtttgcccca ccagcaggac acccgcgcag atcaacacca caccgaccca
     2761 gcgccgcggc gaaaccgact cgtgcaaaaa aataaccgca agcagcgtta gtaatgcata
     2821 tccaaatgca ccagcgggca tgacgtaact gtagtccgcc cagctcagca ccgtcatgta
     2881 actcagcatg aagccggtca gaaagagaat cccaacccag atcgtcccgt tcgtcaccgt
     2941 cagccggaac gcttgcgcaa gccccaaagc cgtcagttgg accgcgccga tctgcgacat
     3001 tccgcgcttc agcatcaggt ccccggcatt ggcgcacacc accatcgcca agaccatgac
     3061 cagcgtcttg attcgcagtc ttctctcgct tgccattttg cgggggttag tcgtttccgg
     3121 aagccgagga cgttcccggc acggaaacca tgggcttttc gaaatcgccg cccttgcgcg
     3181 cccacttcaa gcgctcggcg cgcagtttca ggaattccgt tgcttcgtgg tacaggcgtt
     3241 tccgctcgtg gctgtcccac agcgcttcct tcacgatgcg ccacacaact ttgggccgga
     3301 agtaatactc atcgtagaag cggttcacac ccgacatcat ctccaccttc gagaggtgcg
     3361 ggtactcgat gtgtggcaat tgatggccgc cggaatcagc cagggcttcg atcttcagga
     3421 agccttgctg cgccatggtg tcgtgcaatt ccgttcccgg catggcatgc gccagcgaaa
     3481 cctgaaccgt ctcgcaatcc aattccttcg cgaactcgat ggtcttctga atcgtatctt
     3541 tggtctcgcc tggcaagccg atgatgaagt cgccgtgtac cttgatcccg actttgcggc
     3601 agttcttcat gaaggcgcgt cccatttcaa ccgttgcgcc cttcttgatg ttcttcagga
     3661 tttgcgcgtc gccggattcg aagcccacga taaagagccg cgctccgcca tccgccatcg
     3721 cttttaccgt gtcgtagtcg ctgtgcacgc gcgccgtcga agaccactgg aacttcagcg
     3781 gtttgaactt ggcgcaaaga tccagcacgc ggtccttgcg gatgttgaaa gtatcgtcgt
     3841 cgaagaaaat ctctttcatt tgtggaaagt gttcgagcgt ttgcttcact tcccgcgcca
     3901 cgttttccgt cgaacgcgtc cgccaggcat gtccggaaat cgtctgcggc cacatgcaga
     3961 aggtgcagag cgccgggcat ccgcgcgtgg tgtagaacga cacatagggg tgcagcagga
     4021 acggcacgtt gtagcgttcg atttccaggt cgcgcttgta aatgtccgtg gcgaagggca
     4081 gcgcgtccag ctcttcggtg tgcaattgcg ggcgctccac gttgtgcaca atctttccgt
     4141 ctttgcggta gctgacgccg agaatctcgc tcagcggctt cccgtgcgcg tattccacca
     4201 ccgagtgatc gaactccccg cgcgtcacaa aatcgatgtc cgcgctggca ttcagcgtct
     4261 catcgggctt gatgtgtccg gttggcccga cgaaagcgat cttcatgccc gggttggctt
     4321 ccttgatttt ccggatgagc ttgacgtcgc tcgccaaacc gaccgtggac gtgaagagga
     4381 tcaagaattc gtaatctttg cagatctgaa tcgtctgttg ccaattgact ttgtgaggag
     4441 aggcgtcgag cagcctgctg ccggggatca ttcccgcggg gtacgtcagc caaaccgggt
     4501 accagtagga ttcaatctcg cgcgttgcag gccatcgcga gctcgcaccg ccgtcgaagt
     4561 tttcaaaact cggcggattc aacagcaatg tcttcattac atccggcatt cttcaccctc
     4621 agccttcgct ttacaatcct ggccgtttca gatcgctggc ttctgcgtcc agctttggaa
     4681 tggatggccc gcacacgtac acgcggagct tgcccccaat caccacatac tagtttacct
     4741 gacttcgttg tatcccctca aacgtttctg ttaatgggtg ttccctgcca gcgaacgtct
     4801 atctgcatca ttgaatgggg tttgcgggct ttcgcctgga ggatcagttg cgagcgacga
     4861 ggatctcgtc cgcggataag gagagcaagt cccaaattcg cgttacccaa tcgaggtgct
     4921 ctggtttcat gagcaccgac cgcaggcaag tgagcgtttc gcgatcgcgc ctcatctctc
     4981 ccagattcgc ctcccaaaaa cgaatcggca actccgctac cgccagatgc agatcgcgct
     5041 tcgccgccgc ttcgaatatc ttgcggctta ggacagaagc ctcgctgacg ctcctggcat
     5101 tcggcgcaaa tactacaatg tccaattccg gcgcgaaggg cgtcacaaac cgtgcatccg
     5161 cgcgcacttt ttcgaagagt gccagcgccg ccttccttcc acgttccagc ccgcgcgcaa
     5221 attcgccacc ctttttcagc ggcagcaatc tttgcgtcgc ccacaaggcc gccgcggccg
     5281 cccccggacg cgagcactcc aggctgatct cacccaagtg cagctcggcg gagctgaaat
     5341 aggtgtaggg cgaatcatgc ttgtacaacc gacctacttc ggggtcgcga aacagcacgc
     5401 aaccgcaccc gtagggttgc aatccgtgct tgtgcggatc gatcacgatt gagtccgcct
     5461 gtccaatccg tgcaaaagct cgcttggcat cttctccaag actctccgtg agcgcgaaat
     5521 agccgccgta cgcagcatct gcatgaatcc tgaatccatg ctttgtgcgt aaagcgagaa
     5581 tctctggaag cggatccacg gaacccgttg ccgtcgtacc catagtcgcg acaacgattc
     5641 ctacatcacc gcgctccacc cgtttcgcca gcgcattcac atccatccgg ccccgtgcgt
     5701 cgcacggcac cgtttcgaat ggcaattgca aaacgctgct gatccggccg tgcgtatagt
     5761 gggcctgttc gctggcgaga attttctttc ccggttgcaa ttgccccgcg acccacaaag
     5821 cctcaagatt cgccatcgtt ccgccgccgc acaagtggcc cagaa
//
LOCUS       DASYVO010000321         3461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_248188, whole genome shotgun sequence.
ACCESSION   DASYVO010000321 DASYVO010000000
VERSION     DASYVO010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248188"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VGR03_10860"
     CDS             <1..303
                     /locus_tag="VGR03_10860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEV1994823.1"
                     /translation="DVDDFERAYLKEHLPMARTKLAGATKIALTKIIGSADGSIPPFY
                     QIAEIDFPSIKALQSCAATEGAKETVAHAISISNGGPPLFMIAETETIAMDSRAVV"
     gene            complement(435..2024)
                     /locus_tag="VGR03_10865"
     CDS             complement(435..2024)
                     /locus_tag="VGR03_10865"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEV1994824.1"
                     /translation="MLRRVNFKSGRPVYLQVVDQIKAAAASRALRRGEALPSIGPLAE
                     ELRVNRNAVAKAYSELERLGVIEMLPGQGYFLKENRERLPKEVRRKALTTEIDQAIVQ
                     APLVLQTALMYSLLTILLGALYLGLVVRGKVAAVPAAVVVAAVFLPARSLLQKFAHRL
                     VFAKRFELPRMLRIIKAEAPWQPDLDSFMERVVERTEALLGSRLELIRDRAEVLSLVH
                     SLPSLRSACAPVSAGADLLMPVLSDDEVLGVLRLVPKSTGQEYDAKDLEFLTAVGERV
                     AITANQFRLRNERQESEYALDIQRGLLPREIPQVPGFTIAGAWQPTKMVGGDYYDVFK
                     LSETRLALVVADVSGKGMPAALLMSNLQATAKAYATSESSPKDICDKVNRAICNSITL
                     GKFITFFYAVLDSEGHRLTYTNAGHNPPLIAWHDRACRKLETGGTVLGVFADGSYEQA
                     SIDLLPGDRLVMFTDGITEATDSNGQEFGEERLIALLRESPAASAADLCDAIMQDVTQ
                     FCREDFADDATLLTVVVDRPA"
     gene            complement(2088..>3461)
                     /locus_tag="VGR03_10870"
     CDS             complement(2088..>3461)
                     /locus_tag="VGR03_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994825.1"
                     /translation="IRETGDFSLPAAIPVVSIATFALLCYAGVMLWLGRRTLARFQVT
                     GGMAGDDLLMAVPGVMPEALAGLFRCRPTGPFLNLIRKELRLLRPLWLLTLLFLLYWT
                     CLTMLRPSAVRESFGLVAIALATPALGLGGLIAMLAGCLSLGEERTSGTHSWHMTLPV
                     PARRQWLIKLLMAVFTGFVCTLLLPLLVMAASEFIHGSPVIFFDILSEHHRLDSSSLH
                     RLLIIVPLLSFVSFWCACAVNGMLRAALWVFPAMGAVLLAGVCGDWVGRELAQATVTP
                     MDLVVSWFHFNPWYAIPDGPKVTWLVVPALLFGVIQSYRLFRTQREDSTLSVIRCLLP
                     LALVAFLCSFSVSASGFGDYPRSRWLPFSEAGEAIEKLQLGVTKLDAAHPLQLTVEDL
                     AKSSPMSALTRRWLRGSNIIVAPNQAHSGYFATIHLASGLDCRLAVGHYGGRPVFAWR
                     PCVPRSP"
BASE COUNT          800 a         1026 c         1021 g          614 t
ORIGIN      
        1 gatgtagacg atttcgaacg tgcgtatctg aaggaacatt tgcccatggc ccgtacaaag
       61 ctagcgggcg ccaccaagat tgcccttacg aagataattg gttcagccga cggcagcatt
      121 cctccgttct atcagatcgc agaaattgac tttccttcga taaaggcctt gcaatcttgt
      181 gcggcaacgg agggtgcaaa ggagaccgtg gctcacgcga tctctatttc aaacggcggc
      241 ccacctttgt tcatgattgc cgagactgaa acgatcgcaa tggactctcg cgcggtggtg
      301 tagcggcggg cgactcggtc acgcgttttc gcctttgacg gcgatacacc cggaatgtgc
      361 cgggcgagaa cgacacttct acgcgcaggc gccgtgcagt ggactacgag tttgcgcttt
      421 tcaggaatgc gaacttaggc tggacgatcg acgacgacgg tcaagagggt ggcgtcatcg
      481 gcgaaatctt cacggcagaa ctgcgtcaca tcctgcatga tcgcatcgca caaatctgcc
      541 gcggacgccg ccgggctctc tctcagaagc gcgatcagac gctcctcgcc gaattcctgg
      601 ccattcgaat cggtggcctc agtgatgcca tcggtaaaca tgaccaggcg atccccaggc
      661 aaaaggtcga tgctggcctg ctcgtaagag ccgtctgcga acactcccag cacagtgccg
      721 ccggtttcca gcttccggca agcgcgatcg tgccacgcaa tgagaggggg attgtgtcca
      781 gcgttcgtgt aggtcaaacg gtggccctcc gagtccagca ccgcgtagaa gaaggtaatg
      841 aactttccga gagtgatgct gttgcagatg gcgcgattga ccttgtcgca aatatctttc
      901 ggcgaagatt ccgatgtcgc ataagccttc gctgtggctt gcaagttgga catcaatagc
      961 gcagcgggca tgcctttgcc ggaaacgtcg gcaacaacca gggccagtcg agtctcactg
     1021 agtttgaaga catcgtagta gtcgccgccc accatctttg tcggttgcca ggcgccagcg
     1081 atggtgaaac ccgggacctg aggaatctcg cggggcagca agccacgctg gatgtcaagc
     1141 gcgtattcag actcctgcct ctcatttcgc aagcgaaatt gattggcggt gatggccacc
     1201 cgttcgccga ccgcagtcag gaattccaaa tctttcgcat cgtattcctg acccgtggat
     1261 tttgggacga gccggagaac gccaagcact tcatcatcgg aaaggaccgg catcaataaa
     1321 tcggcgccgg cggaaacggg tgcgcacgcc gagcgcagcg agggaagcga atggaccaga
     1381 gacagcacct ccgcgcgatc ccgaatcagt tccagccggc tgccgagcaa ggcttcggtc
     1441 cgctcgacca ctcgctccat gaatgaatcc agatccggct gccacggcgc ttccgctttg
     1501 atgatccgca acattcgcgg cagctcaaag cgcttagcga agaccaggcg atgcgcaaac
     1561 ttttgaagga ggctccgtgc tggcaggaaa acggcggcca caaccaccgc ggccggtacc
     1621 gcggctacct tgcctcgaac aaccaggcca agatagagcg ccccaagaag aatcgtcaga
     1681 agggagtaca tcagggcagt ttggagcacg agtggcgcct gtacgatggc ttggtcaatc
     1741 tccgtggtca gcgccttgcg ccggacttcc tttggcaaac gctcccgatt ttctttgagg
     1801 aaatagccct ggcccggcag catctcgatc acacccaggc gttccagttc ggaatacgct
     1861 ttggcgaccg cgttgcggtt gacgcgaagt tcctccgcca gcggaccgat ggagggaagc
     1921 gcctcgccgc gtcgcagcgc ccgcgatgct gcggcggcct tgatctgatc caccacctgg
     1981 agatagaccg gcctgccgga cttgaaattg acccgacgta gcatgactgg ggagtctgtc
     2041 atagggcagt tgtccggctg ctgtcaatct cttgatgggc gtgatttcta gggactccga
     2101 ggcacacatg gcctccacgc aaagaccggt ctccctccgt agtgaccgac tgccagcctg
     2161 cagtccaaac cgcttgcaag atggatggtg gcgaaatacc ccgagtgagc ctggttggga
     2221 gcaacaataa tgttggagcc gcgcagccag cgccgcgtga gcgccgacat gggagaagac
     2281 ttggccaggt cctccaccgt gagttgcagc ggatgcgccg catccagctt cgtaacgccg
     2341 agttggagtt tctcaatggc ttcgccggct tcagagaatg gcagccacct cgacctcggg
     2401 tagtcgccga agccggaagc gctcacagaa aagctgcaca gaaacgccac gagggccagc
     2461 ggcagcaggc atcggatcac agacagagtg ctgtcttcgc gctgcgtgcg aaataggcgg
     2521 taactttgga tcaccccaaa gagtaacgcc gggacgacca accaggtcac tttggggccg
     2581 tctggaatgg cgtaccacgg attgaagtgg aaccacgaaa caaccaggtc catcggagtt
     2641 acggtggcct gcgccagttc ccgcccaacc cagtcgccgc atacaccagc aagcaggacc
     2701 gcgcccatcg cggggaaaac ccacagcgcc gcgcgcaaca tcccattgac cgcgcacgca
     2761 caccagaatg agacaaagct cagaagtggc acaatgatta gtaacctatg cagcgacgac
     2821 gaatcgagac gatggtgttc tgacaatatg tcgaaaaata tgacaggcga tccgtggatg
     2881 aattcactag cggccatcac caagagggga agcagcagcg tacaaacgaa acccgtgaac
     2941 acggccatga gcagcttgat gagccactgg cggcgagccg gcaccggcag ggtcatgtgc
     3001 caggagtgcg tccccgaggt tctttcttcc cccagagaca gacatccggc gagcatcgca
     3061 atcaacccac ctaacccgag cgccggcgtg gccagagcta tagccaccaa gccgaacgat
     3121 tcgcgtacgg ctgaaggccg gagcatggtc aggcaggtcc agtacaggag gaaaagcagc
     3181 gtgagcagcc agagcggccg cagcaaacga agctccttgc ggatcaagtt gagaaacgga
     3241 ccggtcggcc ggcagcgaaa caatccagcc aaagcttctg gcatcacgcc tggaacggcc
     3301 atcagcaaat cgtcgccggc cattcctccc gtcacttgga accgcgccag cgttcgccgg
     3361 ccaagccaga gcatcacacc tgcgtagcac aggagggcga aggtggcgat cgaaaccacg
     3421 ggtatggctg ccggcaagga gaaatctccg gtttcacgaa t
//
LOCUS       DASYVO010000322         4603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_122462, whole genome shotgun sequence.
ACCESSION   DASYVO010000322 DASYVO010000000
VERSION     DASYVO010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122462"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VGR03_10875"
     CDS             complement(<1..468)
                     /locus_tag="VGR03_10875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1994826.1"
                     /translation="MNRLSACLITFNEEHNLPRALTSLAGITDEIVVVDSGSTDRTLD
                     IAREFGANVLTHPWSNFGAQKNVAAGATKNDWILSLDADEELSAELQGALLAWKSKEP
                     EFAVYEVARRTWYLGGWVRHTRWYPDYQRKLFLRDKAKFEAVLHATPRFEGPVG"
     gene            complement(568..1101)
                     /locus_tag="VGR03_10880"
     CDS             complement(568..1101)
                     /locus_tag="VGR03_10880"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV1994827.1"
                     /translation="MQTACIHCGTEHLLKDGQFGGHSKVQFKCSKCGKTTIVEIKRRV
                     DSTVVMSPLPSFARADASSSNLQLPPADPGLRLPEKTKVVLSILSGPSKGESHTLAKP
                     RVVLGRKGADIPLNDPEISRHHCLLEVRETYINLKDMDSTNGTFFDEERVRAAMLQDG
                     TEFRIGGSLIRVNFQPK"
     gene            1265..1987
                     /locus_tag="VGR03_10885"
     CDS             1265..1987
                     /locus_tag="VGR03_10885"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1994828.1"
                     /translation="MSDHISRKELKQDRIKETIEHGAEAVISHGQFTLIVVLVLLVGA
                     LVWGGWRFYIDRQTVEASAAFDASMKAYQGRIASAPDPAEPNEPFYPDEPARVQDALQ
                     KFTKVADKYPSTNPGKLARYYSALSLEDLERHNQALEELKKISGSSDKELAAMAQYQM
                     AVIYARTGKPDDAVKTFRALADKQSALVPRPLVLLELAGILRNSDPKEAASIYQKIKK
                     EFPDTAIADQAERGLDTLSPKS"
     gene            2028..3203
                     /locus_tag="VGR03_10890"
     CDS             2028..3203
                     /locus_tag="VGR03_10890"
                     /inference="COORDINATES: protein motif:HMM:NF002326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEV1994829.1"
                     /translation="MTERHIEPVPTPAPWAMRAEESRGRRYPEPTHLYRSAYARDRDR
                     IIHSRAFRRLEAKTQVFTTRYSDHFRNRLTHTLEVAQIARTVASALGLNTELAEALAL
                     VHDIGHPPFGHAGERKLDELMRAQGGCFNHNLHALRIVEQFEQRYLDFPGLNLTFEVR
                     EGIIKHSRDYSAAEFPHLAEYLLDLRPPLEAQLIDWVDEIAYNTADIDDALEAALLDL
                     AVLRKEVSFFGEVYAQVDSAHPGAREKLKFNEALKRVLDALATDLIETSQQRVLASGT
                     SSVEGIRRVPKRLAGFSEVTAARNLELKRFLFSHIYNHPAITEDRNRSVKCLEELFIY
                     YSGRHGSMPASYEELAQTERRHVIVCDYIAGMTDQFLLRRHHEHFGDSSHGKSATPH"
     gene            3229..3675
                     /locus_tag="VGR03_10895"
     CDS             3229..3675
                     /locus_tag="VGR03_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994830.1"
                     /translation="MNEILETLEKTYGPQHATGPTDPYEMIVYLNCGYPASDASCSKG
                     FDALKQEVGLSPKEILAAPKAKLVKLLRLGGIVPELRAARLQEIARKVKAEFGDDLMA
                     VLKKWMQEEKQQTGRGVRGAKKVLQEFPVIGEPTAEKILCFRSWRP"
     gene            3672..3944
                     /locus_tag="VGR03_10900"
     CDS             3672..3944
                     /locus_tag="VGR03_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994831.1"
                     /translation="MTAVPSAFVEVPARLWFGKAGKNYAADYRAARDILSAGLAETFE
                     ARQRAYVLLKKHGQQTCKQSKPKCEICSLTGQCAYIQLQAADRNAV"
     gene            complement(4055..>4603)
                     /locus_tag="VGR03_10905"
     CDS             complement(4055..>4603)
                     /locus_tag="VGR03_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994832.1"
                     /translation="AGWLVPLASAQDHFQVGAYADYFRISQTKSNMAGLGGRAAFKVF
                     PHVMLEGEMSYDFQQAFTEGFTNPSGGAITFQNSNVKVLHGLVGPKIVAGHGAIRPFV
                     TLKGGFVNFRLDPRPASFAGFASSIDNLRANNVSGAIYPGAGLEGHLGPVGLRLEAGD
                     EIYFAGSTHHNLRLAFGPFIRF"
BASE COUNT         1031 a         1408 c         1216 g          948 t
ORIGIN      
        1 tcctaccggc ccctcaaatc taggcgtagc gtggagcacg gcttcaaatt ttgccttgtc
       61 ccgcagaaag agtttccgct gataatctgg ataccaccga gtgtgccgca cccatccgcc
      121 gagataccac gtgcgcctag cgacttcgta taccgcaaat tccggttcct tcgatttcca
      181 agcgagcaaa gctccttgca gttcggcact caactcctcg tccgcatcca gcgacaagat
      241 ccagtcgttt ttcgtcgccc ccgcagctac attcttctgc gctccaaaat tgctccacgg
      301 atgagtgagg acgtttgctc cgaactcccg cgcgatgtcc agagtgcggt ccgtgctccc
      361 cgagtccacc accacgattt cgtcggtaat tcccgcaagc gaagtcaacg cccgcggcag
      421 gttgtgttcc tcattgaacg tgatcaggca ggcggaaagg cggttcatcg gactccgggc
      481 aattttggaa tcgccgctag tatctcacaa agccaatctt gggaatcgaa gcacggagga
      541 accgacttgc gggggagcga agcccggcta cttcggctgg aaattcacgc gaatcaaact
      601 gccgccgatt cgaaactcgg tgccgtcctg caacatggcc gcccgcacgc gttcttcgtc
      661 gaagaaagtg ccgttggtcg aatccatgtc cttcagattg atatacgttt ctcttacttc
      721 cagcaagcag tgatgccgtg aaatttcagg atcattcagc gggatatccg cgcccttgcg
      781 gcccagcacg acacgcggct ttgcgagcgt gtggctctcg cctttcgatg ggcccgacag
      841 aatgctgagc acaaccttcg ttttttcagg cagccgtagt ccgggatcgg ccggcggcaa
      901 ttgcaggttg gaactgctgg cgtccgcgcg agcaaaggag ggaaggggag acatgaccac
      961 ggtggaatcc acgcgccgct taatctccac gatggtggtc tttccgcatt tcgaacattt
     1021 gaactgcacc ttcgaatggc cgccgaactg cccatccttc agcaaatgct ctgtgccaca
     1081 gtgaatgcaa gccgtctgca tgcttcaaaa ctagcaccat ccggaccgct ttggagcacg
     1141 cccgacattt ttcgagcgga aaggttaatc tgcgccccca tatccgcagc ctgcgttccg
     1201 aacccgcctg cgatcgggat cttgtgttac gattacaagt tccagaccac tcaggagagc
     1261 gtgtgtgtcc gatcatattt cgcgcaaaga gctgaagcag gacagaatca aggaaaccat
     1321 cgagcacggc gccgaagccg ttatctctca tggacagttc acgctcatcg tcgtgcttgt
     1381 tcttttggtg ggggcgctcg tctggggcgg ctggcgtttt tatatagatc gccaaaccgt
     1441 ggaggcgtcc gccgccttcg atgccagcat gaaggcctat cagggccgca ttgcctcggc
     1501 tccagaccct gccgaaccca atgagccctt ctatcccgat gaacctgcgc gcgttcagga
     1561 tgctttgcaa aaattcacca aggttgccga caaatatccc agcaccaatc ctggaaaact
     1621 cgcgcgctac tactccgctc tctctctcga agacctcgag cggcacaatc aggcactcga
     1681 agaactgaag aaaatcagcg gaagcagcga caaagagctg gcggccatgg cgcagtatca
     1741 gatggcggtc atttacgcac gcaccggcaa gcccgatgat gccgtcaaaa cgtttcgtgc
     1801 tttggcggac aagcagagcg ctcttgtgcc gcggcccctg gtgctgctgg aacttgctgg
     1861 catccttcgc aattccgacc ctaaagaggc cgccagcatc tatcagaaga tcaagaaaga
     1921 attcccggac actgccattg cggatcaagc cgagcgcggc ctggatactc tttcgcccaa
     1981 gtcctgatcc gctccgcgca taagaattcg ctcggatcag ccggaatatg acggagcggc
     2041 acatcgaacc agttcccact cctgctccct gggcgatgcg cgcggaagag tcccgcggcc
     2101 ggcgctatcc cgagccaacc catctttacc gcagtgccta cgcgcgtgac cgcgatcgca
     2161 tcatccattc acgcgctttt cgccgcctgg aagccaagac gcaagttttc accacgcgct
     2221 attcggacca tttccgcaat cgtctgacgc acacgctcga agttgcgcag attgcccgca
     2281 ccgtcgcctc ggctctgggg ctgaacaccg agctcgccga agcgctcgct ctcgtccacg
     2341 acatcggcca tcccccgttc ggccatgccg gcgaacgcaa gctcgacgaa ttgatgcgcg
     2401 cgcaaggcgg ctgtttcaat cacaatctgc acgcgctccg tatcgtcgaa cagttcgaac
     2461 agcgctatct tgattttccc gggttgaatc tcacgtttga agttcgagaa ggcatcatca
     2521 agcattcgcg cgattattcc gccgcggaat ttccgcacct tgctgaatac cttctcgatt
     2581 tgcgccctcc cctcgaagcg caactcatcg actgggtcga tgaaatcgct tacaacacgg
     2641 ccgatattga cgacgccctc gaagccgcgc ttctggatct cgccgtcttg cgcaaggaag
     2701 tttctttttt tggcgaagtt tacgcgcagg tcgattccgc gcatcccggt gcgcgtgaaa
     2761 aactcaaatt taacgaagcc ctcaaacgcg ttctggatgc actggccacc gatctcattg
     2821 aaacttcgca acagcgcgtc ctcgcctccg gtacctcatc cgtggaaggc attcgccggg
     2881 tcccaaaacg cctcgctggt ttcagcgaag tgacggctgc gcgaaatctt gagcttaagc
     2941 gcttcctctt ctcgcatatc tacaaccatc ccgccatcac cgaggaccgc aaccgctccg
     3001 tcaagtgtct ggaagagctt ttcatttact actcgggccg tcacggctcg atgccggctt
     3061 cctatgaaga acttgcgcaa acggaacggc gccacgtcat cgtttgcgat tacattgccg
     3121 gcatgaccga ccaattcctt cttcgccggc accacgaaca cttcggcgat tctagccacg
     3181 gaaaatcagc aacccctcat tgacggtggg agaaatcatc tgccaaacgt gaacgaaatc
     3241 ctggaaacgt tggagaaaac gtacggaccg cagcacgcaa ccggtcccac ggatccgtac
     3301 gaaatgattg tttacttgaa ttgcggctat cccgccagcg atgcatcgtg ctcgaaaggc
     3361 tttgacgcgc tgaagcagga agtgggacta agtccaaaag agattcttgc ggccccgaaa
     3421 gcaaaactgg tgaagctgct acggcttggc ggaatcgtcc cggagttgcg cgcggcgaga
     3481 ttgcaggaaa tcgcccgcaa agtgaaggct gaatttggcg atgatctcat ggctgtactg
     3541 aagaaatgga tgcaagagga aaaacagcaa acaggcaggg gcgttcgcgg cgcgaaaaaa
     3601 gtcctccagg agtttcctgt gattggagag ccaaccgcgg aaaaaatcct ctgttttcga
     3661 agttggcgcc cgtgaccgcc gtcccctcgg catttgtcga agtgcccgca cggctgtggt
     3721 ttggcaaggc cgggaaaaac tatgccgcgg attaccgcgc cgcacgcgat atcttgagcg
     3781 ccggcctggc ggagaccttt gaggcacggc aaagggccta cgtgctgctg aaaaaacacg
     3841 ggcagcaaac ttgcaagcag tccaaaccga agtgcgagat ttgctcgctg accgggcagt
     3901 gtgcctatat tcagttgcag gccgcggacc gcaatgccgt ctgattccgg atcgatggga
     3961 acagatcgcc ccactttccc gcaatcgaaa aagtggggca atccatcgca ctagaggaat
     4021 tcaacaaagc tgcgctgtta tcccggcgcc gaacctagaa ccggatgaat ggtccgaagg
     4081 ccagacgcag attgtgatgc gtgcttccgg cgaagtagat ctcatcgccg gcctcaagcc
     4141 gcagaccaac cggcccgaga tgtccttcca gacccgcgcc cggataaatc gccccgctta
     4201 cattgtttgc gcgcaagtta tcgatcgaac tagcgaaacc tgcaaagctg gcgggccggg
     4261 gatccagccg gaaattgaca aatccaccct tcaacgtaac gaaaggccgg atggccccgt
     4321 ggccggccac gattttgggg ccaacgagcc catgtaagac tttgacgttg ctgttctgaa
     4381 aagtgatggc cccaccgctc ggattcgtga agccttcggt aaatgcctgt tggaagtcgt
     4441 agctcatctc tccttccagc atcacatgcg gaaacacctt gaaagccgct cgccccccca
     4501 gccccgccat gttgctcttt gtttgcgaga tgcggaagta atcagcgtaa gcaccaacct
     4561 gaaaatgatc ctgcgccgac gccagcggca ccagccaacc tgc
//
LOCUS       DASYVO010000323        18334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_263124, whole genome shotgun sequence.
ACCESSION   DASYVO010000323 DASYVO010000000
VERSION     DASYVO010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 18334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18334
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263124"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="VGR03_10910"
     CDS             <1..1074
                     /locus_tag="VGR03_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994833.1"
                     /translation="YERKILAADERILEIERQLFVEVRSAVAANAARLRRTAAAVAQL
                     DVLTVFAKLAADRGYARPDFNSTGELLIIGGRHPVIEELLRQKGERFVPNDLCFEPGR
                     QQLLLITGPNMGGKSTYLRQSALIVLMAQMGSFVPARQAKLPITDRIFTRIGASDNLA
                     RGRSTFLVEMSEVAVILNHATPASLVLLDEVGRGTSTFDGLSIAWAVVEHLQKHTRAR
                     TLFATHYHELTELGDLLPAVKNIHVSVKETPSEIIFLRRVEPGCADKSYGIEVARLAG
                     LPRSVIERAREVLKRHEQSEHELSETLSPGASDLAHKNGNQQVLFTALDREVLDKLRG
                     ADLDQLKPLDALNLLAELKKQIL"
     gene            1071..2252
                     /locus_tag="VGR03_10915"
     CDS             1071..2252
                     /locus_tag="VGR03_10915"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEV1994834.1"
                     /translation="MSQKPMLVLGMMSGTSADGIDVALARISGAPPNLNAKLLGHTSV
                     KFPPAVCKEILRIAERYPVTAGALSQLNFRLGELFASAAIAACGHFRVRPSKIALIGS
                     HGQTIFHQGKPVNFLGLPTASTLQIGEPAIIAARTGITTVGDFRPSDIALGGQGAPLV
                     PYVDYLLYRHWKLGRVSLNLGGIANITVLPRAVKPQQVFAFDTGPANMLIDALVSHFT
                     GGRRRFDKNARLASQGRSLPALLDDLLRDPYLRIAPPKSTGREYYGHSYVKKLLTLGR
                     RHRAKPNDLVRAATIFTALSVVDALNRFVLPKTKIHQLIVSGGGAHNPLILAQLSAAL
                     PGMEVLPSSRLGIPGDAKEAFAFALLAYETFHQRPANLPSATGARGPAILGKISYAPP
                     R"
     gene            2236..3804
                     /locus_tag="VGR03_10920"
     CDS             2236..3804
                     /locus_tag="VGR03_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1994835.1"
                     /translation="MPRRASRSLSKVRLLITSILPYFIASFCLASCSHSSHRDPSSLT
                     FLIESSPTNLDPRFATDSQSQRIDGLLFSGLLERDNQMNFRGDLAESWSTPDPLTYVF
                     HLRRGVRFHDGRALTSTDVKATFDFILNPDNKSPKRGALRMVTSIEASDDATVIFHLS
                     QPYASFSVNLIPSAIGIVPANAGADFSRHPIGSGPFRFVQQSQDEEVVLERNPEYFRD
                     APQITRVRFRVVPDGVVRALELRKGSADLEMSSLSPDIIPVLARQPDLAVTNRPGTNF
                     TYLGFNLEDPVLSHREIRQALALATDREALIRYLLDGQAKLAAGALPPDHWAAEPKVA
                     QYPYDPARAEQVLENAGFPRKQDGVRLHLTLKCSTEEQARLIGAALQEQWRRAGIQLE
                     LRPLELATLFSDVAKGNFQITYQRWVGANTDPDFFEYAFSSKRFPPDGANRGHYRNAR
                     VDALTDQIRVEMNQEKRKALCSEVQKILAEDLPYLPLWFNDVVSVHRRSLGALDLPST
                     GNYNFLSALHPVGN"
     gene            complement(3840..4475)
                     /locus_tag="VGR03_10925"
     CDS             complement(3840..4475)
                     /locus_tag="VGR03_10925"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEV1994836.1"
                     /translation="MPAELLRVNAQSPETDLLRYAADFIARGSVIAIPTDTFYGLAAD
                     PFNLAAVDEIYRVKGRPETRALPILVNSIEQAIPLAREAPRKFLRLAQAFWPGGLTLV
                     VNASQRIPLKVTAGTGRIALRWPNSTLVRRLIDECEGPVTGTSANISGFPSCSNAEQV
                     MKQLGLRLPLVVDTGETGGTLASTIVELTGDTWRIGREGAIPVAEIEKALE"
     gene            4757..5080
                     /locus_tag="VGR03_10930"
     CDS             4757..5080
                     /locus_tag="VGR03_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994837.1"
                     /translation="MARIRIAKSAAPKWSDYIGAGFEKGLHVFTKRIWLFALDVNRAN
                     HVLACSVEHGNNDLRPCRTKGCEITKIGRDVPNIDCFPGGNCHTSEGIRQNMLKSLLN
                     GYGES"
     gene            complement(5190..5582)
                     /locus_tag="VGR03_10935"
     CDS             complement(5190..5582)
                     /locus_tag="VGR03_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994838.1"
                     /translation="MRKRKITVASLVIVLAIFISSCAAKHPIHPSTANKFDGDSYDVL
                     MATDNIIKTTGQDLTAGKFPPGMDTNVKDALNSLVRVYNEADKVYRAYHDAATAGTVT
                     PAQQAAVTSELNNVSKATNTLKAAKVSK"
     gene            5749..6720
                     /locus_tag="VGR03_10940"
     CDS             5749..6720
                     /locus_tag="VGR03_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV1994839.1"
                     /translation="MPELASLARNLLPSTVMATPGKTARNARPAGDPALLTSRDNRWL
                     KEFRVALRGGLPTENGFVGVEGVRLVEEALRSGCRIQAVLFSESGERHHERLSSFIDR
                     PEMAFPVLRTTDRLFEGLADTEHPQGVAALVHPRETSFDDFVRAPLSACAPLLVVLAG
                     VQDPGNVGTILRTAAAFGATGAATAASGISGTANPFSPKALRASAGAALHLPILVGMS
                     LSILLAQLKVAGVRTLASSVHEPRDDEQALLAPWEVDWCQPVALLVGNEGAGLPEEVE
                     RSADARIRIPMASGIESLNAAAAAAVVFYEAARQRSSHATAQIRPDA"
     gene            6836..8188
                     /locus_tag="VGR03_10945"
     CDS             6836..8188
                     /locus_tag="VGR03_10945"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV1994840.1"
                     /translation="MSLFPHFAKAAEPDVPAANQPLAERMRPRTLDEFIGQEKLLGPG
                     KSLRVQIENDNLGSMLFWGPPGCGKTTLARLIARLTSSEFVSFSAVLAGIKEIKEVMA
                     VAEQKSRSGHRTIVFVDEVHRFNKAQQDAFLPHVEAGHIIFIGATTENPSFEVISPLL
                     SRTKVYVLDPLTTPQIAELLRRALNDKEHGFGNESIEAPDDILFRIASFANGDARAAY
                     NTLELAVRSARPGEKDAQVITPELLEDVLQRKLLRYDKAGEEHFNLISALHKSVRNSD
                     PDAALYWLARMLESGEDPLYLARRMVRMASEDIGLAEPGALAVTLAAKDAFDFLGAPE
                     GHLALAQAVVYLSLAPKSNALYTGYGAVMEDVRKTEADPVPLQLRNAVTGLMKNIGYG
                     EGYKYAHNFDDKVTDMTCLPDNLAGRAYYKPTDQGFEQRLRTRLDEIRKLKSKAARET
                     "
     gene            8402..9094
                     /locus_tag="VGR03_10950"
     CDS             8402..9094
                     /locus_tag="VGR03_10950"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1994841.1"
                     /translation="MIVPLNDEQVVMLWSLPTAPTSDLLVRITFSTDNLAAMKRFLTA
                     KGIAFSAPKGVSEITLSDPEGHRIAFTQYRKGSPNSALVYSALRLIHAGFVVRDRAVE
                     DHFYKDILGFRPYWHGGMKDDKDDWVAMQVPDGTDWVEYMLNISPDADKHTLGVMNHI
                     ALGVPDIQAAKAQLIKNGWKPGEEPKMGRDGKWQLNLYDPDDTRVEFMEFTPKEKPCC
                     SEFTGPHPGPNQ"
     gene            9154..10239
                     /locus_tag="VGR03_10955"
     CDS             9154..10239
                     /locus_tag="VGR03_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994842.1"
                     /translation="MVVGCCSLPARAQNLDKPLQSIDEDITSFAYAPDGRIVYSVHRN
                     FKTKLYDLEHDDIWLQETNGKHRRLLEGQKFNRGNQPFSYIVNSFRWSPNGRMILAEL
                     LTTTVIDDTGKAQDSCQTLILDDGGHEIRIAKGDSVIPDAANPFWLPDNVSINFLSEA
                     VKPRMLFSLKSTRLTTGALKSPHEGRTFRDLVPLPDGNSAIAVEQDRALTGPSRLQRL
                     DLLSDEDKELATLDSYEGGLSVSPSGKMVAYFVDKEILEVRDLTSPSRVARLRIGLGV
                     FRWSPDESRILLKRSLEKKSGDLVWIDLPPLAASSGHDVPVSQPQPVSILHGLSFRDF
                     AISPDGRSLAVVITGKRNLLVFPLPSR"
     gene            10315..10830
                     /locus_tag="VGR03_10960"
     CDS             10315..10830
                     /locus_tag="VGR03_10960"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1994843.1"
                     /translation="MKYRIIFLSTIVGLSLLARPALARQGEKKEASAKTAAPAFDFRG
                     HLQRLYDAWSDLDPAKAASFYAKEASHVFFDIAPLKYTGWAQYAEGVPKAFAAYSSGK
                     FTLGEDLRVHRHGNLAWATATWRGQLLKKDGAKENTEGRYTVVLEKRGEDWLIVHEHM
                     SVPAPPPPATK"
     gene            complement(10913..11353)
                     /locus_tag="VGR03_10965"
     CDS             complement(10913..11353)
                     /locus_tag="VGR03_10965"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV1994844.1"
                     /translation="MRVLLPDGAPDGADAILELLKKYKTIAVVGLSSNPMRPSYGVTE
                     YMQGAGYRIIPVNPNETEVLGEKSYARLEDVPEKIEIVNVFRRAEDVPPVVESAIRIG
                     AKVVWMQLGIENEEAAEKARAVGLIVIEDACILVEHKKRKRSLP"
     gene            complement(11350..12480)
                     /gene="hisC"
                     /locus_tag="VGR03_10970"
     CDS             complement(11350..12480)
                     /gene="hisC"
                     /locus_tag="VGR03_10970"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEV1994845.1"
                     /translation="MTRTIEELIPDYLRGLPVYVPGKPIEEVERELKIHAVKLASNEN
                     PLGPSPKAIAAARAALGESHRYPDGGTQLLREALAKRRGVSREEIFVGLGSSEIIDLA
                     ARVLLRVGLQGLTSEGTYAPFSVAIRASGAELVLVPQREFAFDLQAMAKAITPKTGVI
                     YLANPNNPTGTAFGAKELEEFLASVPDGVLVVLDEAYIHYAVSMGLSDSVEIYRKRKN
                     VLILRTFSKVFGLAGMRIGYAIGQPELFSALNKLRTPFNTSGVAQAAALAALDDVEHV
                     KRGIETNAAERKRLTEGLAKLGFRPVPSEANFVFMEVGPDAKALSDDLLQMGVIVRPL
                     GWMGFPEAMRISVGTAEENDKCLSAMARVILKRAGEGELAAR"
     gene            complement(12625..13350)
                     /locus_tag="VGR03_10975"
     CDS             complement(12625..13350)
                     /locus_tag="VGR03_10975"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEV1994846.1"
                     /translation="MAAERLQKIMAACGIASRRKAEEIIAAGRVTLNGKVVVEQGTKA
                     DPDGDLICVDGKPLKNRERLLYFMLHKPKGYVTTVSDPEGRPTVMELMKKCPERVYPV
                     GRLDYASEGLLLMTNDGALAQKLMKAGSHAPKTYLVKISGKPDEKAIGRLRAGITIEL
                     EDGTRVKTSPAKIRLAEDGANPWYEVILIEGRNRQIRRMFQNVGFLVEKIKRVQLGPL
                     VLDVAPGKYRSLTVREVAQLKSL"
     gene            complement(13386..14174)
                     /locus_tag="VGR03_10980"
     CDS             complement(13386..14174)
                     /locus_tag="VGR03_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEV1994847.1"
                     /translation="MQKIIARAGIASRRHAELLILSGQVRVNGHVVRELGTKADAAKD
                     RIEAAGRVVEAKEGRRVYIVLNKPPEVVSTLADPEGRKTLRNCLRGLPERVFPVGNLE
                     YAASGLVFLTNDGDLAAEMLKNWATLEQVYHVKVKGMLTLADLERLGKEIGVRMQTVR
                     QPDATRGRAANFWYEVRMRDSKKEELRRVLFKEKHPLEKLKRIGLGPLTVEGIPRGRY
                     RLLADKEVEKLRKGENAGKEEDGREEGKKRPLAENTRRTERSQG"
     gene            complement(14350..14544)
                     /locus_tag="VGR03_10985"
     CDS             complement(14350..14544)
                     /locus_tag="VGR03_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994848.1"
                     /translation="MSGMTKTILNAIIHYVPILLGAVVVYSAIFRYPKPPVKLYLRLI
                     YGLIGAVLLYSGIEVLLNSK"
     gene            complement(14607..15341)
                     /gene="scpB"
                     /locus_tag="VGR03_10990"
     CDS             complement(14607..15341)
                     /gene="scpB"
                     /locus_tag="VGR03_10990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEV1994849.1"
                     /translation="MTNEERKAALEAIIYAADEPATIDQLAKALGEEKLAVQASLDEL
                     VASYATEERGIEIRAVAGGYKLYTKPQQHDVVRRFIKSLRPPLRLSMPALETLAVIAY
                     KQPVTSPEISEIRGVNTSGVLSTLLDKHLITTAGRKEVMGRPILYKTSKEFLMRFGLS
                     DLEELPSLKEFEALAREALGSDEGFALADEGLTNEASIGEPAAQSSASAEMEAREELL
                     EETAANNEGMPEAVSDETPKAAAAGE"
     gene            complement(15644..16426)
                     /locus_tag="VGR03_10995"
     CDS             complement(15644..16426)
                     /locus_tag="VGR03_10995"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV1994850.1"
                     /translation="MATPYKINIPMYEGPLDLLLDLIKKQEMNIHDIQISKITAQYLE
                     YLHKLEELDVDVSAEFIYMAATLIYIKSKMLLPPDPLAGPEDSSGDPRAELVQRLVEH
                     EKFKNAAQLLYQKQQIEENVWSKPDKSLYNDEGTEGELVVSLVDLVKVFQHVLERRKE
                     VSRIELRHEEFTVAQMIAALRAQILASADNSVSLIQFFEACPSRHAMIVAFLAVLEMV
                     KLQAVALAQEKQFGEIVVRKHKMFDLVFDESGGIKLIDEEYR"
     gene            complement(16590..17858)
                     /locus_tag="VGR03_11000"
     CDS             complement(16590..17858)
                     /locus_tag="VGR03_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994851.1"
                     /translation="MTTGAEGKKSRVLSGMRPTGRLHIGHYFGALQNWVRLQNDPAYE
                     CFYFIADWHALTSDYADTSAVAENTLQIMIDYLAAGLDPAKSVIFQQSVIPEHAELHL
                     LLSMVTPLGWLERVPTYKEALENVKDKDLHNYGFLGYPILQCADIVIYSKEGTPLLVP
                     VGEDQVSHVEISREIVRRFNSFYGFSLRERLFDKQHREVLGWLCAKFGFWGATEFTPE
                     QKSVLRAELTTRITQAGVKNISEQVPNFESFFDLIEVLTEPDHLLTPTPRIPGLDGRK
                     MSKSYGNAITLSETDEEIRKKTKVMVTDPARKRRTDPGNPDVCPVYDWHKLFSAEEPG
                     RLEWVRQGCTTAGIGCIECKAAMAENLIKWIAPVRERRVKWENDPKGVLEILDEGSRK
                     ARAVAQGTLERVREAVFGWGKKRKEIGGAG"
     gene            complement(18090..>18334)
                     /locus_tag="VGR03_11005"
     CDS             complement(18090..>18334)
                     /locus_tag="VGR03_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV1994852.1"
                     /translation="AWLKDSGERMGAAASKAKLYASEMVNRVVNKAVQVHGSVGYSRE
                     TDVERMYRDARVITIYEGMSEVQRMIIARDLLGKAS"
BASE COUNT         3656 a         6354 c         4292 g         4032 t
ORIGIN      
        1 tacgaacgca aaatccttgc cgccgacgag cgcattctgg aaatcgagcg ccagctcttt
       61 gtcgaggtgc gttctgccgt ggctgcgaac gcagcgcgct tgcgccgcac ggctgcggcc
      121 gttgcgcagc tcgacgtcct caccgtgttc gcgaagctgg ccgccgatcg cggctatgcc
      181 cgccccgatt tcaactccac cggcgaactg ctcatcatcg gcggccgcca tccggtgatc
      241 gaagagctgc tgcgccaaaa aggcgagcgc ttcgttccca acgatctctg cttcgagccc
      301 gggcggcagc agctcctgct catcaccggg cccaacatgg gtggcaagtc cacctatctt
      361 cgccagtccg cgctgatcgt cttgatggcg cagatgggct ccttcgttcc cgctcgccag
      421 gcgaagctgc ccatcaccga ccgcatcttc acgcgcatcg gcgccagcga caatctcgcc
      481 cgcgggcgct cgacctttct ggtcgaaatg agcgaagttg ccgtcatcct gaatcacgcc
      541 actcccgcga gtctcgtcct cctcgatgaa gtcggccgcg gcacgtccac cttcgacggt
      601 ctctccatcg cttgggccgt cgttgaacat ctccagaagc acacgcgcgc gcgcacgctc
      661 ttcgccacgc actatcacga gctgacggag cttggggacc tgctccccgc ggtcaaaaac
      721 attcacgtct cggtgaaaga aacgcccagc gagattattt ttctgcgccg cgtcgagccc
      781 ggctgtgcgg acaaaagcta cggcatcgaa gtggcccgcc tagccggtct tcctcgcagc
      841 gtgattgaac gcgcccgcga agtcttgaag agacacgagc agagcgagca cgaactcagc
      901 gagacgcttt cgcccggtgc ctccgatctt gcgcacaaga acggcaatca gcaggtgctc
      961 ttcacggccc tcgatcgcga ggttctggac aaactgcgag gagccgacct cgaccagttg
     1021 aaaccgctcg acgcgctcaa tctcctcgcc gagctgaaaa aacaaatctt atgagccaaa
     1081 agcccatgct cgttctcggc atgatgtccg gaacatccgc ggatggaatt gacgtcgccc
     1141 tcgcgcgtat ctcgggcgca ccgccgaatc tgaacgccaa gctgctgggc cacacttccg
     1201 tcaaatttcc tcctgccgtt tgcaaggaga tccttcgcat cgccgaacgg tatcccgtca
     1261 ctgcaggcgc tctcagccag ttgaattttc gtttgggaga actattcgcc agcgccgcga
     1321 ttgccgcctg cggacatttc cgagtgcgtc catcgaagat cgctctaatc ggaagtcacg
     1381 ggcaaaccat cttccatcaa ggcaagcctg taaacttttt aggtctgccc accgcttcga
     1441 cgttgcaaat cggtgagccg gccatcattg ccgcgcgcac cggcatcacc actgttggcg
     1501 attttcggcc ctcagacatc gccctgggag gccagggcgc gcctctagtc ccctacgtgg
     1561 actatcttct ctaccgccac tggaaactcg gccgcgtctc gcttaatctt ggtggcatcg
     1621 ccaacatcac ggttcttccg cgtgccgtca agccacaaca agtcttcgcc ttcgacaccg
     1681 gccccgccaa catgctcatc gacgccctcg tctcgcattt cacaggcggc cgccggcgtt
     1741 tcgacaaaaa cgcccgcctc gcatcacagg gacgaagcct ccccgcgctc ctcgacgatc
     1801 tgttgcgcga tccctatctc cggatcgctc cgccaaaatc caccggccgc gaatactatg
     1861 gccactccta cgtaaaaaaa ctcctcactc tcggccgccg ccatcgcgcc aagcccaacg
     1921 atctggtccg cgcagccaca attttcaccg cactctcggt cgtcgatgct ctgaaccgct
     1981 tcgtcttgcc caaaacgaaa attcaccaac tcatcgtttc cggtggaggc gcgcacaatc
     2041 cgttgatcct cgcccaactc tccgccgccc ttcccggcat ggaagtcctg ccctcatcgc
     2101 gtctcggcat ccccggagac gccaaggagg ccttcgcctt tgccctgctc gcttacgaaa
     2161 cttttcacca acgccccgcg aaccttcctt ccgcaaccgg cgcccgcggc ccagcgatcc
     2221 tcggtaaaat atcctatgcc ccgccgcgct agccgctcac tctcgaaggt ccgtcttctc
     2281 attacctcaa tccttcccta cttcattgct tcgttctgtc tcgcatcatg ttcacattcg
     2341 tcccaccgcg atccttcttc cctcaccttc ctcatcgaat ccagccccac caatctcgat
     2401 ccgcgctttg ccaccgattc gcaatcccag cgcatcgatg gactgctctt cagcggattg
     2461 ctggagcgcg acaatcagat gaattttcgc ggcgaccttg cggaatcctg gtccactccc
     2521 gacccgctca cgtacgtttt ccatctccgc cgcggtgttc gcttccacga tggccgcgcg
     2581 ctaacctcca ccgacgtcaa agcgacattc gacttcatcc tgaatcccga caataaatcc
     2641 cccaaacgcg gcgcccttcg catggtcacc tccatcgaag cgtccgacga cgccacggtc
     2701 atctttcatc tcagccagcc gtacgcttcc ttcagcgtga atctgattcc ttccgccatt
     2761 ggcatcgttc ccgcgaacgc cggcgccgac ttttcccgcc accccatcgg ctccggcccc
     2821 tttcgcttcg ttcagcagtc gcaggacgaa gaagttgtcc ttgagcgcaa tcctgaatac
     2881 ttccgtgatg cccctcagat cactcgcgtc cgattccgag tcgtccccga cggcgtggtt
     2941 cgcgccctcg aactccgcaa aggctctgct gatctggaga tgagctccct ctctccagac
     3001 atcattcccg ttctcgcgcg ccagcccgat ctcgcagtga cgaaccgccc cggcacgaat
     3061 ttcacctacc tcggcttcaa cctcgaagat cctgtcttgt cccatcgcga aattcgccaa
     3121 gcgcttgctc tagccaccga ccgcgaagcg ctcatccgct atctcctcga tggccaggca
     3181 aagctcgcag cgggcgcgct gcccccggat cactgggccg ccgaaccgaa agtcgcgcag
     3241 tatccttacg atcctgctcg agctgaacaa gttctcgaga acgcgggttt ccctcgcaaa
     3301 caggatggcg tgcgcctgca tctcactctg aaatgttcca cggaagagca agcccgcctc
     3361 atcggcgcag cgcttcagga gcaatggcgc cgcgccggca tccagctcga actccggccg
     3421 cttgagctcg ccacgttgtt ctctgacgtc gcaaaaggaa attttcagat tacctaccag
     3481 cgctgggtcg gcgccaacac cgatccggac ttttttgaat atgctttctc gtcgaagcgc
     3541 ttcccgcccg acggcgccaa tcgcggacac tatcgcaacg cgcgcgtcga cgcgctcacg
     3601 gaccagattc gcgtggaaat gaatcaggaa aaaaggaaag ctctatgcag tgaagtccag
     3661 aaaattctcg cggaagatct cccctatctg ccattatggt ttaacgacgt cgtttccgtc
     3721 catcgccgct cgctcggcgc gctcgatctt ccctctaccg gcaactacaa cttcctctcg
     3781 gctcttcatc cggttggaaa ctaattaacg agcgccgcct cgcgccgccg agttttacac
     3841 tattccaacg ccttctcaat ctccgccacc gggatcgcgc cttccctgcc gatcctccaa
     3901 gtgtcgccag tcaattccac gatggtcgaa gccagcgttc cgccggtttc tcccgtgtcc
     3961 accaccagcg gcaagcgcaa cccgagttgc ttcatcacct gttccgcgtt cgagcacgac
     4021 ggaaatccgg aaatgttagc gctcgttccc gtgaccggac cttcgcattc atcgatcaag
     4081 cgccgcacca gcgtgctatt cggccagcgc aatgcaatgc ggccagtgcc ggcggtcact
     4141 ttgaggggga tgcgctgcga cgcgttcaca accagcgtca gccctcctgg ccagaatgcc
     4201 tgcgcgagcc gcagaaattt acgcggcgct tcccgcgcaa gcggaatggc ttgctcgatt
     4261 gaattcacga ggatcggcaa cgctctcgtc tccgggcgcc ccttcacgcg gtaaatttca
     4321 tccaccgcgg ccagattgaa cggatcggcc gccagcccgt agaaagtgtc cgtgggaatc
     4381 gcaatcacac ttccgcgggc gataaaatcc gccgcatacc gcaacaggtc cgtctccgga
     4441 gattgcgcat tgacgcgaag cagttcagcc ggcaattttt tggcctttgg cgaagtcgtg
     4501 acgccaccct gcagcgcccc gccagcgctg cccttatttg ggcgacgcta ccataccgcc
     4561 tcaacccacg caataagagc ctgctggtga acctctcccc gcagccaaag cgggccgcga
     4621 aatgacaaac attggtctgg gatcgccagt ttccggcaag ccaaccatat agccaaatgg
     4681 ccgccaactt cagaggagaa gccccaaagc gcagggatag tttccgattg ggcgactatc
     4741 cgggagttat cgcctggtgg ctcgaattag aatcgcgaag tcagcggcac caaagtggtc
     4801 ggattacatc ggcgcaggct tcgagaaggg cttgcacgtc ttcactaaac gcatttggct
     4861 tttcgctctc gacgtcaatc gtgccaacca cgttctcgcc tgctcggtcg agcacgggaa
     4921 caatgatctc cgaccttgtc gtaccaaagg ctgtgagata acgaagatcg gtcgcgacgt
     4981 ccccaacatt gactgttttc cgggcggaaa ttgccacacc agcgagggca ttcgccaaaa
     5041 tatgctgaaa agcttgctca atggctatgg ggagagctag ccctggaata aaagacaacc
     5101 ccccaacggg cgcgttgatg atagctagga tgatatctag cttcgactgg cctgtagtta
     5161 gcagcttata tatttactat taagtatact tatttcgaca cctttgctgc ttttaaagta
     5221 ttcgttgctt tcgatacatt gttcagctca gaagtcacag cagcctgctg tgcgggagtt
     5281 actgtccccg ccgtagctgc atcatggtaa gctctgtaga ctttatctgc ttcattataa
     5341 acccgaacga gggagttgag cgcatcctta acattcgtgt ccattccagg tggaaacttc
     5401 ccagcagtta agtcctgccc ggtggtctta ataatgttgt cagtggccat caacacgtcg
     5461 taggagtcac cgtcaaactt attagctgtg ctagggtgaa tggggtgttt tgctgcacag
     5521 cttgagataa agatcgctag cactattact aagcttgcta ctgttatctt tcgctttctc
     5581 atccttcctc ctttaggcgc taggcctatt tgctatccta tctgcctctt ctggtgaaat
     5641 tgttgaacgt gaatcaaggg tcacgttaat gacgtagtga gaaccgcacc tactacaagt
     5701 gatcccactc tcccatgagt cagggagttt agtactattg aacgaaacat gccagagtta
     5761 gcgtcccttg ccagaaattt gctaccctcc acggtaatgg ctacgccagg caaaaccgct
     5821 cgcaacgctc gccccgcggg cgatcccgcg cttctcacca gccgcgataa tcgctggctc
     5881 aaggaattcc gcgtggctct acgcggcggc ctgcccacgg aaaacggctt cgtcggcgtc
     5941 gaaggcgtca ggctcgtgga agaagctctg cgttccgggt gccgcattca agccgtgctc
     6001 ttcagcgaat ccggcgaacg ccatcacgaa cgtctctcct ccttcatcga ccgccccgaa
     6061 atggcttttc ccgttctccg caccaccgac cgcctcttcg aaggtctcgc cgacaccgaa
     6121 catccgcaag gtgtcgccgc gctggttcat cctcgggaga cctccttcga tgatttcgtg
     6181 cgcgccccac tttctgcttg cgctccgctg ctcgtcgtac tggccggcgt ccaggatccc
     6241 ggcaatgttg ggacgatcct gcgcaccgct gccgcctttg gcgcgaccgg cgccgccacg
     6301 gctgcttccg gcatcagcgg cacggcaaac cctttctcgc cgaaagctct gcgcgcctcc
     6361 gcaggcgctg cgttgcatct tccgatcctc gtggggatgt cgctatcgat tctcctcgcg
     6421 caactcaagg tcgcaggcgt tcgcacgctc gcttctagcg ttcacgaacc gcgtgacgat
     6481 gagcaagctc tgctcgctcc gtgggaagtc gattggtgcc agcccgtggc ccttcttgtc
     6541 ggaaacgagg gtgccggtct tcccgaagaa gtcgagcgca gcgccgatgc gcgcatccgc
     6601 attcccatgg ccagcggcat cgaatccttg aatgccgccg ccgcggctgc agtcgttttt
     6661 tacgaagccg cccgccagcg gagttcgcat gctacagccc agattcggcc cgacgcctga
     6721 ggtttcttat ggggttcaac ttctgcaagc gcgatagtat tggcaaggtg ttacggcgat
     6781 cgctttttta ccttgagcac agccgaaggg ctgaagcggg cgcgtttaag gagttatgag
     6841 cctcttccct catttcgcta aggctgccga gcctgacgtt cccgctgcca accagccgct
     6901 cgccgagcgc atgcgcccgc gcacgctcga tgaattcatc ggtcaggaaa aacttctcgg
     6961 tcccggcaag tcgctgcgcg ttcagatcga aaacgacaat cttggctcca tgctcttctg
     7021 gggcccgccg ggctgcggga agaccacgct cgcgcgcctc atcgcgcgcc tgacaagttc
     7081 cgaattcgtt tctttcagcg ccgttttagc aggcatcaag gaaatcaagg aagtgatggc
     7141 cgtcgccgag caaaaatccc gctccggcca tcgcaccatc gtcttcgtcg acgaagttca
     7201 ccgcttcaac aaagcccagc aggacgcgtt tctacctcac gtcgaagcgg gtcatatcat
     7261 cttcatcggt gcgaccaccg agaatccttc cttcgaagtg atctcgccgc ttctttcccg
     7321 caccaaggtt tacgtcctcg atccgctcac cacgccgcaa atcgctgaac ttctccgccg
     7381 cgccctgaac gacaaagagc acggcttcgg aaacgaatcc atcgaagctc ccgacgatat
     7441 cctcttccgc atcgcttcct tcgcgaatgg tgacgcgcgc gccgcctaca acacgctgga
     7501 gcttgccgtt cgtagcgcgc gtccgggcga aaaagacgcc caggtcatca cgccggaact
     7561 tctcgaagac gtgctgcaaa ggaaactcct ccgctacgac aaagccggcg aggagcactt
     7621 caatctgatt tctgccctgc acaaatccgt ccgcaactcc gatcccgacg ccgctctcta
     7681 ctggctcgcg cgcatgctcg aatccggcga agatccgctc tatctcgctc gccgcatggt
     7741 gcgtatggcc agcgaagaca tcggtcttgc cgagcccggc gctctcgcgg ttacgctcgc
     7801 cgccaaagac gctttcgatt tcctcggcgc gcccgaaggc catctggccc tcgcccaagc
     7861 cgtcgtctat ctctcgctcg cgccaaaatc gaacgctctc tacactggct acggcgctgt
     7921 catggaagac gtccgcaaaa ccgaagccga ccccgtcccc ctccaactcc gcaacgccgt
     7981 caccggcctc atgaaaaata tcggctacgg cgaaggctac aagtacgccc acaattttga
     8041 tgataaagtc accgacatga cctgcctccc cgataatctc gccggccgcg cctactacaa
     8101 gcccaccgac cagggcttcg aacaacggct gcgcactcgt ctcgacgaaa ttcgcaagtt
     8161 aaaatcaaaa gcggctcgcg aaacctaagg tgacacgatg aagcgcctaa ctctcttctt
     8221 cacggctctg cttttttcca tgtcttgtcc ggcccaggag gctccaaaga gacctcgcat
     8281 cctcgggatt tctgaggtcg acatgtacgc cacaaacgct gcagctactc gaagtttcta
     8341 taggaaagtg ctggacgcga gtggtccgtg ccactggtgc gaggagattc ctcgctcgcc
     8401 catgatcgtg ccgctaaacg acgagcaggt cgtgatgctc tggagtttac caacagcgcc
     8461 cacctcagat ttattggtcc ggatcacttt ctcgacggat aacctagctg ccatgaaacg
     8521 attcttaacg gctaagggga tcgcattttc ggcgccaaag ggggtatcgg agattactct
     8581 cagtgaccca gaaggtcatc ggattgcgtt cacccagtac cggaaggggt caccgaactc
     8641 ggcgctggtt tattcagcgt tgcgtctcat ccatgctggc ttcgtggttc gcgatcgcgc
     8701 cgtcgaagac catttctaca aggacattct cggattccgt ccctactggc acgggggaat
     8761 gaaggacgac aaagacgatt gggtcgccat gcaagtccct gacggcacgg actgggtcga
     8821 atacatgctc aacatctcac ccgacgcgga caaacatacg ctcggtgtga tgaaccatat
     8881 cgctctgggt gttcctgaca ttcaagccgc aaaagcgcaa ctcatcaaga acggttggaa
     8941 gccaggcgaa gaaccgaaga tgggccgcga cggcaaatgg cagctcaatc tttacgatcc
     9001 cgacgacact cgcgtcgaat tcatggaatt cacaccgaag gaaaaaccct gttgttccga
     9061 attcactggt ccgcaccccg gccctaacca atgacctgtc cctgttcccg cagccgcttt
     9121 cgctgggttc gtcaaatttg cggtctggtt tgcatggttg tggggtgttg ctccctgccc
     9181 gctcgcgccc agaatctcga caagccactc caatccatcg acgaagacat cacttccttc
     9241 gcttacgccc ccgacggccg catcgtttat tccgtccacc gcaacttcaa aaccaagctc
     9301 tacgacctcg aacacgatga catctggctt caggagacaa acggcaagca tcgccgcctg
     9361 ctcgaaggcc agaaattcaa ccgcggtaac cagcccttca gctatatcgt caattccttc
     9421 cgctggtctc ccaacggccg catgatcctc gccgagcttc ttaccaccac ggtgatcgac
     9481 gacaccggca aggcgcaaga ctcctgccaa accctaatcc tcgacgacgg cggccacgag
     9541 attcgtattg ccaaaggcga cagtgttatt cccgacgccg cgaatccgtt ctggctcccc
     9601 gacaatgttt ccatcaactt cctttcggaa gccgtgaaac ctcgaatgct tttttccctg
     9661 aagtccacac ggctcaccac cggtgctctc aagtcacccc acgaaggacg caccttccgc
     9721 gaccttgtcc cgcttcccga tgggaattcc gccatcgccg ttgaacaaga tcgcgccctc
     9781 accggcccgt cccgtctgca gcgcctggac ctgctcagtg atgaggacaa agagttggcc
     9841 acacttgaca gctacgaggg cggcctcagc gtctctccta gcggcaagat ggtcgcctat
     9901 tttgtcgaca aggaaattct cgaggttcgc gatctcactt cgcccagtcg tgtagcccgc
     9961 cttcgcatcg gcctcggcgt cttccgctgg tcccccgacg aatcgcgcat cctcctcaag
    10021 cgttccctcg agaaaaaatc cggcgacctc gtctggatcg atctccctcc gctcgccgcc
    10081 tcatcggggc acgacgtccc tgtctcgcag ccgcagcccg tttccatcct tcacggcctc
    10141 agcttccgcg atttcgccat ctcccccgat ggccgctccc tcgccgtcgt catcaccggc
    10201 aagcgcaatc tcctcgtctt cccgctgcct tcgcgttgac attgaacgac caccgatgta
    10261 ggttctgccc acgggaaaga ctcgcctcaa aaagccattt agcacggagg acttatgaaa
    10321 taccgcatca tctttctctc gactatcgtt gggctttcgc tcctcgccag gcccgcgctg
    10381 gcaagacaag gagaaaagaa agaagcctct gcgaagacgg cggcgcctgc cttcgacttt
    10441 cgaggtcact tgcaaaggct ctatgacgct tggtcggacc tcgatccggc gaaagccgct
    10501 tccttttatg caaaagaggc gagccacgtc ttcttcgaca tcgccccgct caaatatacg
    10561 ggctgggcgc agtatgccga gggagtccca aaggcctttg ccgcctactc ttcaggaaaa
    10621 ttcacacttg gcgaagatct aagggttcac cggcatggca acctcgcttg ggctacagcc
    10681 acctggcgcg ggcagctcct gaagaaagac ggcgccaagg agaacacgga aggacgctac
    10741 accgttgttt tggaaaagcg cggagaggac tggctcatcg tccatgaaca catgtcggtg
    10801 ccagcgcctc ctccgccagc cacaaagtaa actgcgagcg cctccggatg ctttaaccaa
    10861 gttgtcttga cgacttggct ctaagcgggc ccggcctgtt ggaaccaaat cgctagggca
    10921 aactgcgttt ccgcttcttg tgttccacaa gaatgcacgc atcttcaatc acgatcagcc
    10981 ccacagctcg cgctttttcc gccgcctctt cgttctcaat gcccagctgc atccaaacca
    11041 ctttcgcgcc gattcgaatc gcgctctcca ccaccggcgg cacatcctcc gcgcgccgga
    11101 acacgttcac gatctctatt ttctccggca catcctccag ccgtgcgtaa ctcttctccc
    11161 ccagcacttc cgtctcgttc ggattcaccg gaatgattcg atatcccgca ccctgcatgt
    11221 attccgtaac gccataactg ggccgcatcg gattcgaaga caatcccacc accgcaatag
    11281 tcttgtattt tttcaggagt tcgagaatcg cgtcagcgcc atccggcgcg ccatccggga
    11341 gcagcactct catcgcgccg cgagttcgcc ttcgcccgcg cgcttgagga tcacccgcgc
    11401 catggccgac aaacatttgt cattttcttc cgccgtcccc accgaaatgc gcattgcttc
    11461 cggaaatccc atccacccca gcggccgcac gatcacgccc atctgcagca gatcatcgct
    11521 cagcgccttc gcgtccggcc ccacctccat aaaaacgaaa ttcgcctcgc tcggcaccgg
    11581 ccgaaatccc agcttcgcca atccctcggt cagccgtttc cgctccgctg cattcgtctc
    11641 gatgccccgt ttcacgtgtt ccacgtcgtc cagcgcggcc agcgccgcag cctgcgccac
    11701 gcccgacgta ttgaatggcg ttctcaattt attcagcgcg gaaaatagct ccggctgtcc
    11761 gatggcgtag ccgatgcgca tccccgccag tccaaaaact ttcgaaaacg tcctcagaat
    11821 cagcacgttc tttcttttgc ggtaaatctc tactgaatcg ctcaggccca tgctcaccgc
    11881 gtaatggata taagcttcat ccagcaccac cagcacgcca tccggcacgc tggcaagaaa
    11941 ttcttccaat tctttcgcgc caaacgccgt ccccgtcggg ttgttcggat tcgccaggta
    12001 gatcacgccg gtcttcggcg tgatggcctt cgccatggcc tgcaagtcaa acgcgaactc
    12061 ccgctgcggc accagcacta gttccgcccc gctcgccctg atcgccaccg aaaacggcgc
    12121 atacgtccct tccgacgtca gcccctgcaa tcccaccctc aaaagaaccc gggctgccag
    12181 atcgataatc tcgctcgatc ccagccccac aaaaatctct tcccgtgaaa ctccccgccg
    12241 cttcgccagc gcctctcgca agagctgtgt accgccgtcc gggtatcggt gcgattcccc
    12301 aagcgccgct ctcgccgccg caatcgcttt cggtgaaggc cccagcgggt tctcattcga
    12361 agcgagcttc accgcatgaa tctttagctc ccgttccact tcttcaatcg gttttcccgg
    12421 aacgtacacc ggcagcccgc gcaggtaatc cggtatcagc tcttcaatcg ttcgcgtcat
    12481 ctcttccagt ctacttcccc aattgttttg tcgccaatcc gccgctcacc gatgtggcca
    12541 ttctcttacg gtcgtggttg ccaagtcggt gccgcgggcc gctcctcatt gtagtggccg
    12601 gtcttcagac cggcgttttt cattctatag actcttcaat tgcgccactt cccgcaccgt
    12661 cagcgagcga tattttcccg gagccacatc cagaaccaac ggccccagtt gcacgcgctt
    12721 gattttctcc accagaaatc ccacgttctg aaacatccgc cgaatctgcc gattccgccc
    12781 ttcgatcaga atcacctcat accagggatt cgccccgtcc tccgccagcc gtattttcgc
    12841 cggcgaagtc ttcacccgcg tcccatcctc cagttcaatc gtgattcccg cacgcaatcg
    12901 gccaatcgct ttctcgtccg gctttccgct gatcttcacc agatacgttt tcggcgcgtg
    12961 cgaaccggct ttcattagct tctgcgccag cgctccatca ttcgtcatca gcagcaatcc
    13021 ttcgctggcg tagtccagtc gccccactgg ataaacgcgc tccgggcatt ttttcatcag
    13081 ctccatgacc gtcggccgcc cctccggatc gctcacggta gtcacgtaac ctttcggctt
    13141 gtgcaacatg aaataaagca gccgctcccg gttcttcagc ggcttgccat cgacgcaaat
    13201 caaatccccg tccggatcgg ccttcgtgcc ctgttccacc accacttttc cgttgagcgt
    13261 cacccgcccc gcggcaatga tctcctccgc cttgcgccgc gacgcaatcc cgcaagccgc
    13321 cataattttc tgaagtcttt ctgcagccat cgtgcgtgga ttctcctgcg aggaaaaact
    13381 gtagctcacc cctgcgatct ctctgttctc cgcgtattct ccgctaaagg tcgctttttc
    13441 ccttcctctc gcccgtcctc ttccttcccc gcattctctc cctttcgcaa tttctccact
    13501 tccttgtccg cgagcaatcg atatctcccc cgcggaattc cctccaccgt cagcggcccc
    13561 aatccaatgc gcttcaactt ttccagcgga tgcttctcct tgaacaacac gcgccgcagc
    13621 tcttcctttt tcgaatcccg catccgcact tcgtaccaga aattcgccgc gcgcccgcgc
    13681 gtcgcatccg gctgccgcac cgtttgcatc ctcacgccaa tctcctttcc cagccgctcc
    13741 aggtccgcaa gcgtcagcat tccctttact ttgacgtgat agacctgttc cagcgtcgcc
    13801 cagttcttca gcatctccgc cgccaggtcc ccgtcattcg tcaaaaacac gagtccagac
    13861 gccgcatact ccagattccc cactggaaac acgcgctccg gcaatccgcg caaacaattt
    13921 cgcagcgtct tacgcccttc cgggtctgcc agcgtcgaca ccacttccgg cggcttatta
    13981 agcacgatgt aaacccgccg cccctccttc gcctcaacga ctcgccccgc cgcctcaatc
    14041 cggtccttcg ccgcatccgc cttcgtcccc agctcccgca ccacgtgccc gttcacgcgc
    14101 acctgtccgc tcaggatcag cagctccgca tgccgccgcg aagcaatccc cgcccgcgca
    14161 attatcttct gcaatctttc ctgcatttgc tggatttcgt ctttccggaa cagtttttcg
    14221 ctaaggcact tgttgcattt cagatgtgca cttatgcacc gtcattccga gcggagcagg
    14281 cggagtcttt ttcttcccca tcgctcctcc gatgcgtcgg cctgcgtaat gaggaatctc
    14341 ggtagtgggc tactttgaat ttagcagcac ctcaatgcca gaatacagca aaacagcgcc
    14401 tataagaccg taaatgaggc gcaaatacaa tttcaccggc ggcttgggat agcgaaagat
    14461 ggcggaatag acgaccacgg caccaagcag gatcggaaca tagtgaataa ttgcgttgag
    14521 aatcgtctta gtcatgccgc tcatttcgcc acctcttcgg attacttcct tatttcatta
    14581 tttcattgcg tctttacttc gtcttttcat tcccccgccg ctgccgcttt cggcgtttca
    14641 tcgctcaccg cctccggcat cccctcatta ttcgccgccg tctcttccaa caattcctcc
    14701 cgcgcttcca tttccgcgct cgctgagctc tgtgccgcag gctccccgat gctagcttcg
    14761 ttggtcagcc cttcatccgc cagcgcgaat ccttcatcgc tccccagcgc ttcccgcgcc
    14821 agcgcctcaa actccttcaa gctcggcaat tcctccaaat cgctcaaccc aaaccgcatc
    14881 aaaaactctt tcgacgtctt gtacaggatc ggccgcccca tcacttcttt gcgccccgcc
    14941 gtcgtgatca agtgcttgtc cagcagcgtg ctgagcactc ccgaagtatt cacaccgcga
    15001 atctccgaaa tctccggcga cgtcaccggc tgcttgtacg caatcaccgc cagcgtctcc
    15061 agcgccggca tcgacagccg caacggtggc cgcaagctct tgatgaaccg ccgcaccacg
    15121 tcatgctgct gcggcttggt atacagcttg tatccgcccg ccaccgcgcg aatctcgatc
    15181 ccacgctcct cggtcgcata gctcgccacc aactcatcca gcgacgcctg caccgccagc
    15241 ttttcctcac cgagcgcctt cgccaactga tcgatcgtcg ccggctcatc cgccgcgtaa
    15301 ataatcgctt ccaacgccgc ttttctctct tcatttgtca taaacagtta ctccaatttc
    15361 ggtttgtcgg tttgcagcta ggccttgatt ttaggcggtc ttcccttgcc gcctggccgc
    15421 cacggcttgt accccttctt cctccgcgca agcagcctgt ttttcttggc acattcgtca
    15481 cacgatgcgg catagtgatt gagcggcgca gagcacagtt cacattttcc ctctgcgcgc
    15541 cgggctctct gccacaaccg ctgccttgat tgggtcttca cgtcacatct ctccctccgc
    15601 agtctcgcaa accgtctcct cgctcctcgt cgtccgccac ttcctaccga tactcctcat
    15661 caatcaactt gatcccacca ctctcatcaa aaaccaaatc aaacatctta tgcttccgca
    15721 ccacgatttc cccaaactgc ttctcctgcg ccagcgccac cgcctgcagc ttcaccatct
    15781 ccagaaccgc caggaacgcc acgatcatcg cgtgccgcga cggacacgcc tcaaaaaact
    15841 gaatgaggct caccgaattg tccgcgctcg ccagaatctg cgcccgcagc gccgcaatca
    15901 tctgcgccac ggtaaactct tcgtgccgca gctcgatccg cgaaacttcc ttccgccgct
    15961 ccagcacatg ctggaacact ttcaccaaat cgaccagcga aaccaccagc tcgccctcgg
    16021 tcccttcgtc gttgtagagc gacttgtccg gcttcgacca cacgttctct tcaatctgct
    16081 gcttctggta caaaagctgc gccgcattct tgaacttttc gtgctccacc aagcgttgca
    16141 ccagctccgc ccgcggatca cccgacgagt cctccggccc cgccaaagga tcaggcggca
    16201 gcagcatctt gctcttgatg tagatcagcg tcgccgccat gtaaataaat tccgccgaca
    16261 catccacatc caactcttcc agtttgtgca ggtactccag gtattgcgcc gtgatctttg
    16321 agatctggat atcgtgaata ttcatctcct gcttcttgat cagatcaagc agcaggtcca
    16381 gcggcccctc atacatcggt atattaattt tgtacggcgt cgccattttg tagttctctg
    16441 ttctcagttt tcagccatca gttttcaatc gccactaaaa gacaagaatt aacacagagt
    16501 ttgcggagtt cggagcacag agggcacaga gaagaaccga aaaaagcgga ttcctcgcgt
    16561 cgctcggaat gacagttgac tctcatgctt tagcctgctc caccaatttc ttttctcttc
    16621 ttcccccagc cgaacaccgc ttcccgcact cgctccaatg ttccttgcgc aactgcgcgc
    16681 gccttccgcg acccttcatc taatatctcc agcacgccct tcgggtcgtt ctcccacttc
    16741 acgcggcgtt cgcggactgg ggcgatccac ttgattaaat tttcggccat ggcggctttg
    16801 cactcgatgc agccgatccc tgccgtcgtg cacccttgcc gcacccactc caaccgcccc
    16861 ggctcttccg ctgagaacag tttatgccag tcatacaccg gacacacgtc cggatttccc
    16921 ggatcagtcc gcctcttccg cgccgggtcc gtcaccatca ccttcgtctt cttccgtatc
    16981 tcctcatccg tctcgctcaa cgtgatcgca ttcccataac tcttcgacat cttccgccca
    17041 tccagccccg gtatcctcgg tgtcggtgtc agcaaatggt ccggctcggt caaaacttcg
    17101 atcagatcga aaaacgactc aaagttcggg acctgctcag agatattttt gacaccggcc
    17161 tgtgtaatgc gtgtggtaag ttcggcccgg agaaccgatt tttgttccgg cgtaaactcg
    17221 gtcgctcccc aaaatccaaa tttggcgcaa agccagccca acacttcacg atgttgtttg
    17281 tcgaataacc gttcgcgcag agagaatcca tagaaggaat tgaacctccg cacaatttcg
    17341 cgcgaaatct cgacgtgcga aacctgatct tcccctaccg gcaccagcag cggtgtgcct
    17401 tccttgctat aaatcacaat gtccgcgcat tgcagaatcg gatagcccag aaatccgtaa
    17461 ttgtgaaggt ccttgtcctt aacgttctcc agcgcctctt tgtacgtggg caccctttca
    17521 agccacccca gcggcgtcac catggacagc aacaaatgca actccgcatg ctccggaatc
    17581 accgactgct gaaaaatcac gctcttcgcc ggatccagcc ccgccgccag ataatcgatc
    17641 atgatctgca gcgtattctc cgccaccgcc gaagtgtccg cgtaatcgct cgtcagcgca
    17701 tgccaatccg caataaagta gaaacactca taggcagggt cattctgtaa ccgcacccaa
    17761 ttctgcagcg cgccgaaata atggccaata tgcagccgcc ctgtcggccg catcccgctc
    17821 aacaccctgc tcttctttcc ttcagcccct gtcgtcacca cgcaccccta agcctaattc
    17881 gccgtaccaa aacacaagcg aagagagatt tctctctgca caggccgacc ggttcgcagg
    17941 agcgaaccgg agagaaatat cggcctgttc cgctcgaaat gacaatttct tcttacgggt
    18001 tcaacggccc gtaaggaaac gtgcaaaagc gcgcccgtac aacgtcctct tctccgcgac
    18061 agcgccgggc taaagcccgg cctctacaac taactagcct taccaagcaa gtcgcgcgcg
    18121 ataatcatcc gctggacttc gctcatgccc tcataaatcg taatcacccg cgcatcccgg
    18181 tacatccgct ccacgtccgt ctcccgcgaa taccccacgc tgccatgcac ctgcaccgct
    18241 ttattcacca cgcgattcac catctcgctg gcatacagct tcgccttgga cgccgccgcg
    18301 cccatccgct cgcccgaatc tttcaaccac gccg
//
LOCUS       DASYVO010000324         4538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_37136, whole genome shotgun sequence.
ACCESSION   DASYVO010000324 DASYVO010000000
VERSION     DASYVO010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37136"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(341..577)
                     /locus_tag="VGR03_11010"
     CDS             complement(341..577)
                     /locus_tag="VGR03_11010"
                     /inference="COORDINATES: protein motif:HMM:NF014809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HEV1994853.1"
                     /translation="MKGAPANPAPAAATHHTALEVAWTVAPAMILAVIAIPSFHLLPH
                     LLRAGMAMTVTASNAIGPIPILKIKVAASNSIPG"
     gene            complement(1182..1499)
                     /locus_tag="VGR03_11015"
     CDS             complement(1182..1499)
                     /locus_tag="VGR03_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994854.1"
                     /translation="MRTHPAGAVIASEAKQSSLLPPLVDCFVASLLAMTPLAMSRRPR
                     DLVLRVDTKAPRNSAANTIVCSARRLHATSPGGLPEEAAARMDARDEAWRKARAGEPA
                     RPE"
     gene            complement(1816..3489)
                     /locus_tag="VGR03_11020"
     CDS             complement(1816..3489)
                     /locus_tag="VGR03_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994855.1"
                     /translation="MRSFRKASAGSNVNHQGIEPRRMNTDPTLLAKASSGQSPLSDDL
                     AIRASVGCSGGWWLPFVGLVAALLGLPFIRTVFGMGDEGVLLRGAERMLQERKLYTDF
                     FEFLPPGGFVLTATWFSIAGISVWSARLLAISTIVGIACFTYVACRQASRNAPLSAIF
                     TIGWVVMSQGRWTQVSHHWFTTLFSMVGVWATLASFEHSPRWLRWPLIAGAAAGTAAM
                     VTPTRGALAMLAATTAFLNLRRDRTELIAYVLGGAMVPAGLLIYLVSNQALAAAFDDV
                     IRFTAARYSSIQGVPFGSFSDAQNLPLKYLFPLAALVTLLVCVREWRTCLRDRLLRSC
                     IAFGLAGFVGCFPRPDITHIAFAAPLALPLLACCMTRLAQRWRQIYRYVVAAVLIGLC
                     APCAVSFLWISQIALRGQVVQTPRGGVVFSGQPGVSELLARIAATPPGDAYFFYPYMP
                     MLPFLAAREDVSGYDIFAPGYTLPSQYQNACISVMQRASWVVIDRRWTDPNTWRQVFP
                     AMQDARPQETKEFEQALDSGFEFVARDGTFELRRRHEGIGDTFCAGIAE"
     gene            3874..>4538
                     /locus_tag="VGR03_11025"
     CDS             3874..>4538
                     /locus_tag="VGR03_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994856.1"
                     /translation="MTAGAILLGFAIRTTVGADAPLWLDETFTGAIAAQLSLWSVVHQ
                     ILQDVNAPIYCLLAHAWSLLFTLSNQSLRFPALVFGSIAPLLCLIPSPGITRRTRLLW
                     CSLIALWIPGFLYSQEARCYSLLLCLAIGSTIAFVRLIDEPNSKRASIWALLGSLSIL
                     THYHALILIGFQGLAYVGLHRGRALRSWPAALVFLPVFAWLFVHSGRIAEFADPHIAW
                     YAR"
BASE COUNT          921 a         1368 c         1354 g          895 t
ORIGIN      
        1 gccgcgtcat tggccgcgaa atggcctagc gcggggtcta tcgcggcgta tttcttcctc
       61 gttccggcga gccacgccgc atagctttcc tcgctcacgc caggaaaagc aatcggcata
      121 ttggaatggt ccttgccgca aagcttggaa cattgaccat attggaacat tgaccataat
      181 tgatgccctc gcgttcggcc ttgaaccaga ttccttcatc cggcggggaa cagcctcgat
      241 gcggtcactt cgacgcgccc aatttttcct gccggcacga cggcgtcgtt atccacggag
      301 agctggggat gtcgcccggc ttcaggtccc gcggcctgta tcatcccgga atcgaattcg
      361 aggccgccac cttgatcttt aggataggga taggtccaat agcattgctt gcggtcaccg
      421 tcatggccat gcccgcccgc agcaggtggg ggagcagatg aaacgatgga attgcgatca
      481 cggcgaggat catcgccggg gccacggtcc aggcgacttc gagagcggta tgatgcgtgg
      541 cggcggcggg agccggattg gctggcgcgc ctttcatttc ttcctcaatc ttttgaggcg
      601 gtgggtccgt tggttcaaaa ccacaaacgc ccatcgcgcg caagcggccg cattgcggca
      661 taaacgcgcg ttgaaagcgc tgcttggaaa ggccgcgcgc gctggaaagt tcactccgta
      721 tcggggagag gcgcttcgat agcatccatc gctcccaccg ccgcctcttt gtctgcggaa
      781 aataatgcgc agcttgcggt cagcctcgcg atgttgcgcc aatgtcacac cggcaactca
      841 aatgccgggt gagatggtat cggaggtttg acgcaatcgg cgctcgtgct agacattcat
      901 gatggctggt aggaaacaaa caattacact gggaattgcc ctggcgctcg cggttctcgc
      961 cgtcgtcgca gtggtgtgga ttgggttgcc gcttttggtg ttagcggtgt tgttatttgc
     1021 gtggggtttg gaacccaaac gcacggagga atttgttggg cgtttgccgt tgggcaatta
     1081 cctcctcaag gcattggcta aattagactt gataatctcc cgctggagtt gagagagccc
     1141 taacggcggc ctccctcgat tacgcggatc tatcgccgtg ctcactccgg ccgcgccggt
     1201 tccccggccc gcgcctttcg ccaagcctcg tcacgtgcgt ccatccgggc cgcagcctcc
     1261 tccggcaagc cgccgggcga tgtcgcgtgc aggcggcgcg ccgaacaaac gatcgtattc
     1321 gcggctgaat tgcgaggggc tttcgtatcc acccgtaata ccaagtctcg tggacggcga
     1381 ctcatggcga ggggcgtcat tgcgaggagc gaagcgacga agcaatccac aagcggcgga
     1441 agcaaactgg attgcttcgc ttcgctcgca atgacggctc ctgcaggatg ggttctcatc
     1501 agtaatatct ggtttaaggg gaacgttact gtttttaagg catatcgaga actccgcaca
     1561 ttcgagaagg gtcggtgatg ccgagcgtga ttgcgatgga gatcggcgcc gccgcgaaga
     1621 tcgacgtaac gatcgtcatg ccttgtctaa acgaggcaaa atggctgccc gcctgtatcg
     1681 ccaatgccaa ggaggcgctc acccgcattc gggcggagct cggcttgaac ggtgaaatcc
     1741 tcatcgacga caacggcagc gcggacagat cgcaagggcc ctcggcgcga cgctcgtcga
     1801 ggtctcgcga gcaatctact ccgctatgcc ggcacagaag gtgtcgccaa tgccttcgtg
     1861 gcggcgacgc aactcgaacg tcccgtcccg ggctacgaac tcgaaaccgc tatccagggc
     1921 ttgctcgaat tctttcgttt cttgcggccg agcgtcctgc attgcgggaa acacctgcct
     1981 ccatgtgttg ggatctgtcc atctccggtc gatgaccacc caagaagcgc gctgcatcac
     2041 cgatatgcag gcattctgat actgggacgg cagggtgtat cctggcgcaa agatatcgta
     2101 tccagacacg tcctcgcggg cagccaggaa tggcagcatc ggcatgtacg ggtagaaaaa
     2161 ataggcatcc ccggggggcg tcgcggcgat ccgcgccagc aactcagaaa ccccgggctg
     2221 tccggagaac accaccccgc ctcgcggcgt ctgcacgacc tgcccacgca acgcgatttg
     2281 ggatatccac aagaatgaaa ccgcgcaggg cgcgcaaagc ccgatcagga cagcggcaac
     2341 cacgtagcga tatatttgac gccaccgttg ggcaagccgg gtcatgcagc atgcgagcaa
     2401 gggcaacgcc aaaggtgccg caaaggcgat atgagtaata tcggggcgcg gaaaacaccc
     2461 gacgaagcct gcgagaccga acgcgatgca cgaccgcagc agacggtcgc gaagacacgt
     2521 gcgccactcg cggacgcata cgaggagcgt caccaaggcc gcgagcggga ataggtactt
     2581 gaggggaaga ttctgggcgt ccgaaaatga cccgaacggt acgccttgga tggacgaata
     2641 ccgcgcggcg gtgaatcgga tgacatcatc gaatgctgct gcgagcgcct gattcgaaac
     2701 caagtatatg agcaggcccg cgggcaccat ggcgcccccg agtacgtagg cgatcaactc
     2761 cgtccggtcc cgccgcaggt tcaagaacgc cgtcgtggcg gccagcatag ccagagcgcc
     2821 gcgggtcgga gtcaccatcg ccgccgtgcc agccgctgcg ccggcaatta gcggccaccg
     2881 cagccagcgc ggcgaatgtt cgaagctggc gagcgtggcc cagacaccca ccatcgagaa
     2941 aagtgtcgtg aaccagtgat ggctgacctg cgtccacctt ccctgcgaca ttaccaccca
     3001 cccgatcgtg aaaatggcag agagcggcgc gttcctggat gcctgccggc aggccacata
     3061 cgtgaagcag gcaatgccga cgatagtcga gatcgccagc aaccgcgcgg accaaacgga
     3121 gatcccggct atactgaacc atgttgcggt gaggacgaag ccgccgggcg gcagaaattc
     3181 aaagaaatcc gtgtagagtt tcctctcctg gagcatgcgc tcggcgccgc gcaacaatac
     3241 tccctcatcg cccatcccaa agacggttcg aatgaacggc aggcccagca gggcggcaac
     3301 cagacccaca aacggcaacc accagccgcc gctgcagcct acagatgccc ggattgccaa
     3361 gtcgtcggat aatggggatt ggccagagct tgcctttgcc agcagcgttg ggtcggtgtt
     3421 catgcggcgt ggctcgattc cctgatgatt gacgttcgaa cccgcagatg ctttccggaa
     3481 ggagcgcatg tccgaccgtt tcttgtatag gccggattgt caatcggctt cgaatttgac
     3541 ccccgatcgg agtgcgcccc tgcggccgga catcttaggc ctagggaatc gatctggtag
     3601 ctgggctttt tgaggatgaa agccatggcc agacaccgtt cccatagcat cgcgttcaaa
     3661 caccagtcgc catcagacgc cgcggaaatc gcaaaaatcg cgtccaattg gtgaagtctg
     3721 gttaagggta gatcaatcaa acaaccgtaa agtccggcga cgatcagctc gacagaagag
     3781 agcggacgcg ggcggatgtt cacgaaagcc acatctcaat ttctttcggt cgaggggagg
     3841 cttcgagtct cccttccgga tccgttggtc gcgatgaccg ccggagccat cctgctcggc
     3901 ttcgcgatcc gcacgactgt aggcgccgac gcaccgcttt ggctggacga aacgttcaca
     3961 ggcgcgatcg ccgcgcagct ctcactctgg agcgttgtcc accagatcct tcaggacgtc
     4021 aacgctccca tctactgcct gctggcgcat gcctggtcgc tgctttttac cctgtcgaac
     4081 cagtcgcttc ggtttcctgc gctggtattc ggatcgatcg cacctctgct gtgcctgatc
     4141 cccagcccag gaatcacccg ccgcacgcgc ctactctggt gctcgctgat agcgctgtgg
     4201 attcccgggt ttctatattc gcaggaggcg cgctgctatt ccctattact ctgtctcgcg
     4261 attggcagca cgattgcttt cgtcagacta atcgacgagc cgaattcgaa gcgagcctca
     4321 atctgggcgc ttctcggctc tctgtccatt ctgacgcatt atcacgcgct gatcttgatc
     4381 ggctttcagg gtctcgccta tgttggcctc caccggggcc gcgccctgcg aagctggccg
     4441 gcggcgctcg tttttcttcc cgtctttgcg tggctcttcg tccatagtgg acgtatcgcg
     4501 gagttcgcgg atccacacat cgcctggtat gcgcggat
//
LOCUS       DASYVO010000325         6275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_275976, whole genome shotgun sequence.
ACCESSION   DASYVO010000325 DASYVO010000000
VERSION     DASYVO010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275976"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VGR03_11030"
     CDS             complement(<1..266)
                     /locus_tag="VGR03_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV1994857.1"
                     /translation="MKRNYLVLGIVTLAVAGALLAQDPPAKTTKEKAGSHGSSGSAVK
                     GKEVFEKKCAMCHFADSDAKKIGPGLKGISKRGTFTVNGNKVTT"
     gene            473..955
                     /locus_tag="VGR03_11035"
     CDS             473..955
                     /locus_tag="VGR03_11035"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1994858.1"
                     /translation="MSMETLTRAWEVLQDAYQAQMEGDYDRAVELYQSSLELHPTAEA
                     HTFLGWTYHFQGRVEEAIAECKRAIELDPEFGNPYNDIGAYLIELGRFEEAIPWLERA
                     TEARRYEPRHFPHYNLGRAYLGKEMYSHAMRCFQEALEIEPRYSLARQALASLRRMVN
                     "
     gene            complement(1031..2305)
                     /locus_tag="VGR03_11040"
     CDS             complement(1031..2305)
                     /locus_tag="VGR03_11040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1994859.1"
                     /translation="MSFSQRLDEIRTGFERPFWVANISELFERLSYYAAFASLARYLH
                     ETLAFPTERAADLTGLFGGLVWFLAIFGGTLADRLGFRRALSLAYLILAGSYFLLGSI
                     GAPWLAPVRNAMPLVVFVTFVLTLPALGVALVKPCVVGTTARASAENVRSIGYSIYYT
                     LVNIGGFIGPLVASYVHKNLSVENVFRAAAVSVFLMFFAVLFFFKEPRKANEQAATTI
                     GETLRNFVKVITNPKFMLFLLIFSGYWIVYWQEFITLPLYVHDYIDRTADTEKMLSIG
                     PLVVIALTVAVSVLTQKIAAFRAVILGTFIAMIAFAILAIHPTVIAAYVTLAVVALGE
                     LTQQPRYYDYISRLAPAGQQGTYMGFAFLPLGIGAFLAGRIGGKLLHHFGEVQHQPQR
                     FWWALTAIGIGTTLLLWIYDRVVKPSGQEASS"
     gene            2983..4602
                     /locus_tag="VGR03_11045"
     CDS             2983..4602
                     /locus_tag="VGR03_11045"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEV1994860.1"
                     /translation="MSLLTSSPVASPSEPRPEEFPIEAGEADAPQLNPTPTLKTNMAA
                     HEVRVTATGAPPDKSTGERELFTEETTSVLVFENGGVIRLSAAVAPGQLLFLTNVESK
                     REVVAQVKRKRAYRPTICYVELEFAEPAPRFWGMEFSAAAALLPKDAQDAQAAELVIT
                     AEATADEPGEPPAAPSVEEVQGLKREVEALRGQLKLMQTPATSQQAPARVPTAVPEAS
                     PTLAAGDVPSTESKANGGSDEASGGAFTEKSLPIEHKPVPMQLTAEEPAQLPKPSLDF
                     SRSLPKAKRSLRARGSFTPSFRGGALRLALLTTALALTAAGVAWYKHWIPWKSATKKP
                     SVSVPAIALNAKTSPLPGSQAAAQVHPEFRDTKVASDAPVTSSGMPSRSAALPNTLPP
                     EPTDAVEPSAQPFASSSSVAQPAVRKTAPSTSLAENRSTVRPTTRAASDSAAPSAVKG
                     VFVPPKLLKSVRAVASLDALRDFETGNVVIDAVVGSAGEVNFISVLSGPSSLRAAAVE
                     SLKQYQYEPATRNGQPVPSHVTITIHFRFEP"
     gene            complement(4886..5869)
                     /locus_tag="VGR03_11050"
     CDS             complement(4886..5869)
                     /locus_tag="VGR03_11050"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEV1994861.1"
                     /translation="MKRQVWLYLILAYVLSWLIWIIAQHFGVGPDREEYIAAFGVAGP
                     ALAAVFLSRRGQDGAGEQLPARLLSFALLWLFAWAIYIANDKLRGIHAPSSVSYYLFV
                     GLLATIPAWILSGAFTRDSGVRELLQTLVHPGNWRWQAAAFFFWPVILLIPAAIAHLF
                     HVPLLWPQHRDTVWHSAAYAGISFLSNFLFTAALEEPGWRGFLLPHLQQRFSPLLASL
                     FVWLPWALWHAPLDFHQPFRSSLQQYLLLRVAFLIPITIILTWFYNRSGANLLTVVIF
                     HAAMNTFSFVLPYYPPAFALLILFAVIVIFTDRMWRLRPGVFTGAVNGAQP"
     gene            5997..>6275
                     /locus_tag="VGR03_11055"
     CDS             5997..>6275
                     /locus_tag="VGR03_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994862.1"
                     /translation="MKRLMIVVMTVAGALSAGAVMAQKPPLLPEKDVAALANELSGET
                     AKRNLEGIARFHRQRGSQGFHEAAELVAERLRAYGLSDVAILQFPADGK"
BASE COUNT         1461 a         1637 c         2037 g         1140 t
ORIGIN      
        1 tccgtcgtga ctttgttgcc gttcacggtg aaagttccgc gcttggaaat gcctttgagt
       61 cccggcccga ttttcttcgc gtcgctgtcc gcgaaatggc acatggcgca tttcttttcg
      121 aacacttcct tgcctttcac ggcggaccct gatgatccgt ggcttcctgc cttctccttc
      181 gttgtctttg ctggcggatc ttgcgccagg agtgctccgg ccacagccag agtgacgatg
      241 ccaagaacca gataattccg cttcacgatg ccccctcgtc gaattggtcg ttatcgctaa
      301 atgatgaagc cacgctggaa cccagctatt ctctccattt gcggtcccaa tttccacctc
      361 ttctcccggt atgcctaccc ccaggcccgt cacaaggtac aggttcctct cctgcacgcc
      421 cgggactttc gtacttttga cccccgcaaa accatgtgct aactatgtgt ccatgagcat
      481 ggagaccctc acgcgggcgt gggaagtcct acaggacgct tatcaggcgc agatggaagg
      541 cgactacgat cgcgccgtcg agctttatca atcgtctttg gagctgcatc ccaccgccga
      601 agcccatacc tttttggggt ggacatatca tttccagggg cgcgttgaag aagccattgc
      661 ggaatgcaag cgcgccatcg agctcgaccc ggagtttggc aatccttaca acgatatcgg
      721 cgcgtatctg atcgaactgg gccggttcga ggaagcgatt ccttggctcg agcgcgccac
      781 ggaagcgcgc cgctacgagc cgcgccactt tccccattac aacctgggcc gcgcgtatct
      841 cggaaaagag atgtacagcc acgcgatgcg ctgtttccag gaagcgctgg agatcgagcc
      901 gcgctattcg ctggcacggc aggcgctcgc ttctcttcgc cgcatggtga attgaaataa
      961 agtaatcagt catcagtggt cagttttcag ttcagaagaa tcatcctccg ttttccaagt
     1021 tctatcgaat tcacgaggac gcctcctgcc cggatggctt gacgacgcgg tcgtaaatcc
     1081 acagcaacag agttgttccg ataccgatgg cggtaagcgc ccaccagaaa cgctgcggct
     1141 ggtgctgcac ttcgccgaag tggtggagaa gcttgccgcc tatgcggcca gcgagaaaag
     1201 cgccgatgcc gagcggcagg aaagcgaacc ccatataggt gccctgctgg ccggcagggg
     1261 cgaggcgcga aatgtagtcg tagtaacgcg gctgctgcgt gagttcgcca agtgcgacga
     1321 cggcaagagt gacgtaggcg gcgataactg tcggatggat ggcaagaatc gcaaaagcga
     1381 tcatggcgat gaacgtgccg aggatcacgg cacggaaggc ggcgattttc tgggtgagga
     1441 cgctaacagc gacggtcagt gcaatgacga cgagagggcc gatggagagc atcttctcgg
     1501 tatcggcggt acgatcgatg taatcgtgaa cgtagagcgg aagagtgatg aattcctgcc
     1561 agtaaacaat ccaatatccc gaaaaaatca gaaggaagag catgaacttg ggattggtga
     1621 tgactttgac aaaattgcgc agcgtttcgc cgatagtggt ggcggcctgc tcgttcgcct
     1681 tgcgcggttc cttgaagaag aagagcacgg cgaaaaacat caagaagacg ctgacagcag
     1741 cggcgcgaaa aacattctca acgctgagat tcttatggac gtaggaagcg acgaggggcc
     1801 cgatgaaacc accgatattg acaagcgtgt agtagatcga atagccgatg gaacggacgt
     1861 tttcggctga agcgcgagcg gtggtgccaa ccacgcaagg ctttaccaat gccacgccga
     1921 gtgctggaag agtcagcacg aaggtaacaa agacgactag cggcatagca ttgcgcacgg
     1981 gggccagcca gggcgcgccg atcgagccga ggagaaaata ggagccagcc aggatcagat
     2041 aggcgagtga gagggcgcgg cgaaaaccga gacggtcagc gagcgttcca ccgaagatgg
     2101 cgaggaacca gacgaggccg ccgaaaaggc cggtgagatc cgcagcacgt tctgtaggaa
     2161 aagcgagcgt ctcgtgaaga tagcgggcga gagaagcgaa cgcagcgtag taagagagac
     2221 gctcgaagag ttcgctgatg ttggcgaccc aaaaggggcg ctcgaagccc gttcgtatct
     2281 cgtcgagacg ctggctgaaa ctcatttctc ccccggatgg aagcgcagcg attgtactgg
     2341 agcgaggtag agaagcaagg aagtaaggag gtaaagatag gggcggacgt ggcccctcca
     2401 cgttcccgga agattcgatg cgggatgcta gcagagccag gggtaccccc catgagatgc
     2461 atgaatttca aaacaaaaga gttgcagtct aggggacatt tttatcgagg tcagaagggg
     2521 acattatcaa agaggtttaa catgattact cgtaagtggt ttgtttgtca gggggcggcg
     2581 ggctgtctgt ccgagttggc gccgggaaaa gcggaatagc gcaggttttt agcggtaagc
     2641 agaccgagtt gtgcaggttc ccaagactct gtttctggac tcggatgtcg gtgaaccact
     2701 tgtccggtaa cgcgactaac cggacgagta gctttccctt gcgcggtaac ccgttcacga
     2761 tcttgcttgt gggcacgaac gaagtggaat ttctgaggcg acctgattgt actcaatgag
     2821 gcacgcagta cttttgtacc atggtaaagg ggccagccga gcagtgagaa tggaggggtc
     2881 tggaccgtcc gcaaggcgga cgattctccg caacctgaat ttcgcccggg acctctcgca
     2941 atcccggcac aagggacgac caaattgtct tccgcagagg acatgagtct tttgacttcc
     3001 tcgcccgtgg cgagtccttc agagccgcgg cccgaggagt ttccgatcga ggcgggagaa
     3061 gccgacgcac cccagttaaa tccgacacca actttaaaaa cgaatatggc cgcgcatgaa
     3121 gtacgtgtga cggccaccgg agcacctccc gacaagagta cgggcgagcg cgagcttttc
     3181 accgaagaga cgacttccgt attggtcttt gaaaatggcg gagtgatccg gttgtctgcg
     3241 gccgtggcgc ccggacaatt gctctttctc acgaatgtgg aatcgaagcg cgaagtcgtc
     3301 gcgcaagtga agcgcaagcg cgcgtaccgg ccgacgattt gttacgtgga gctggaattc
     3361 gctgaacctg ctccccgctt ctggggcatg gagttttccg cagcggcggc gttgttgcca
     3421 aaggacgccc aggatgcgca agctgccgag ctggtgatca cagcggaagc tactgccgat
     3481 gagcctggcg aaccgcccgc tgcgcccagt gtcgaggaag tgcaggggtt aaagcgggaa
     3541 gtggaagcac tgcgggggca actgaagctg atgcaaacgc ccgccacgag tcagcaagca
     3601 ccggcacggg taccaactgc ggttccggaa gcgtctccga cattggcggc gggggatgtg
     3661 ccgagcacgg aatcaaaggc aaacggtgga tcggacgagg catctggcgg tgccttcaca
     3721 gagaaatccc tcccgatcga acacaagcct gttccgatgc aattgacagc cgaggagccg
     3781 gcgcaactgc cgaagccttc gctggatttt agcaggtccc tgccgaaggc caagcggtcg
     3841 ctccgggcgc gagggagttt cacgcccagc tttcgcggag gagcgttgcg tctggcgctg
     3901 ctcaccacgg cgctggcact gactgcagcc ggcgtggcgt ggtacaagca ctggattccg
     3961 tggaagtcag cgacaaaaaa accatcggtc agtgtacccg cgatcgcttt gaatgcgaag
     4021 acatcgccgt tgccgggaag ccaagcggcc gcgcaggtgc atccggaatt cagagatacg
     4081 aaagtggcga gtgatgcgcc tgtgacctca tcaggcatgc catcgcggag cgcggcgctg
     4141 cccaacacac ttccgccaga accgacggat gcagttgagc cctctgctca acccttcgca
     4201 tcgagtagtt ccgtagcgca gccggctgta aggaaaacag ctccctctac aagcctggcg
     4261 gagaatcgtt ccacggttcg tccaacgacg agagccgctt ctgactccgc tgcgccttct
     4321 gcggtgaagg gcgtcttcgt gccgccgaag ctgctcaaat cggtgcgagc cgtggcttcc
     4381 ttggatgccc tgcgggactt cgaaacggga aacgtcgtga tcgacgcggt cgtgggctcg
     4441 gcgggcgaag tgaacttcat cagcgtgctc tcgggacctt cgtcgttgcg cgccgccgcc
     4501 gtggaatcct tgaagcaata ccaatacgag cccgccacac ggaacggcca gcctgttccg
     4561 tcacacgtca cgatcaccat ccatttccgg ttcgaaccgt aagcctggct cagggcgtgt
     4621 acgcgttggc gcaggaactt cggcgttctt gttggcgtga gtcgcaggag ggcttagcga
     4681 gtctctgcca attcacggta acactgccat tcctgaatgg cagcgcactc ggaaataatc
     4741 cacttggatg tgtaacgcta gcgttaacca gaaaaatgaa atttcccggc acccccgaag
     4801 tcccggtgtc ggaagttcga gcacaacaca gtgtcttgcc ctgggccgca aatcggccgg
     4861 gaccgcagag cactaacgac gcgcatcacg gctgcgcccc attgacggcg ccggtgaaaa
     4921 cgccggggcg gaggcgccac atgcggtcgg tgaagatgac gataaccgcg aagaggatga
     4981 ggagggcaaa agcgggcgga tagtagggga ggacgaagga aaatgtgttc atggcggcgt
     5041 gaaagatgac gacggtaagc aaattggcac cggaacggtt gtagaaccag gtgaggatga
     5101 tagtgatggg gatgaggaag gctacgcgga ggagcagata ttgctgcagc gaggagcgga
     5161 aaggctgatg gaagtcgagc ggggcatgcc agagcgccca gggaagccaa acgaagagac
     5221 tggcgaggag cggcgaaaaa cgctgttgga ggtgaggaag aaggaatccg cgccagccgg
     5281 gttcttcgag ggcggcggtg aacaggaaat tgctaaggaa agagatgccg gcgtaggcgg
     5341 cggagtgcca aacggtgtcg cgatgctgcg gccacagcag tggcacgtgg aagagatgcg
     5401 cgatggcagc gggaatgagc agaatgacgg gccagaagaa aaacgcggcg gcttgccagc
     5461 gccaattgcc gggatggacg agagtttgca ggagttcgcg gacgccggag tcgcgcgtga
     5521 aggcgccgga gagaatccat gcggggatcg tcgccaggag tcctacgaac agatagtagg
     5581 agacggaaga cggagcgtgg atgccgcgga gtttgtcgtt cgcgatgtag atggcccagg
     5641 caaacagcca aagtagcgcg aaagaaagaa ggcgagcggg aagctgctcg ccggcgccgt
     5701 cctgaccacg gcgcgaaagg aagacggcgg cgagtgcggg gccggcaacg ccgaacgcag
     5761 caatatattc ttcccggtcc gggccgacgc caaaatgctg cgcgatgatc cagatcagcc
     5821 aggacaacac ataggcgagg atgaggtaga gccagacttg tctcttcatg tagcgggaga
     5881 catatcacca aaatgagaag acggcaattg gagatattaa aagaggccgc tcgaggcggc
     5941 aacaaaaggt agaattcccg gcggaatgcg ggtcgcggag acgtggggag ggacatgtga
     6001 aaaggctaat gattgtggtg atgacggttg ccggtgcgtt atcggcgggc gcggtgatgg
     6061 cacagaagcc gccgctgctg ccggagaaag atgtggcggc gctggcgaat gaattgagcg
     6121 gagagaccgc gaagcggaat ttggagggga ttgcgcggtt tcaccggcag cgcgggtcgc
     6181 agggatttca tgaggcagcg gaattggtgg cggagcgttt gcgggcatac ggattgagcg
     6241 atgtggcgat cctgcaattt ccggcggatg ggaag
//
LOCUS       DASYVO010000326         8409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_135154, whole genome shotgun sequence.
ACCESSION   DASYVO010000326 DASYVO010000000
VERSION     DASYVO010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135154"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VGR03_11060"
     CDS             <1..241
                     /locus_tag="VGR03_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HEV1994863.1"
                     /translation="NLLGVNDGTTTRDISVPMAVLIGDTTGNGAVNSSDIAQTQSQSG
                     QLVSASNFREDVTANGQINSSDIALVQSKSGTALP"
     gene            368..940
                     /locus_tag="VGR03_11065"
     CDS             368..940
                     /locus_tag="VGR03_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994864.1"
                     /translation="MRTDQPRINVRRAALIFVAGIITTSCSEKPAPDREKDVNASEAP
                     REIVRESEVETAEGGDEPQPGKDEIEEDCAAFVRSTKVVPARAAATDCPGCPAGGTAV
                     LAFRSMKTDVVSCSGDTCTVVVTIRAVFNPGSGETVTGGLTAWIPAEQRNAYLSGHTP
                     SDEQVYRVRITYKRSGAAWRAVEFDRAPGE"
     gene            1040..1429
                     /locus_tag="VGR03_11070"
     CDS             1040..1429
                     /locus_tag="VGR03_11070"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV1994865.1"
                     /translation="MAHASVSSIAATLIALACMTAVAETPAPGTSGIEGMIVVSPTRP
                     GPIRKDEELSVAPVSNAQFVVKAGDATVKTFTTDGDGRFQVGLPPGHYVILREGAAPR
                     VGRWRFEADVVAGQMTKVNWTADSGMR"
     gene            1635..5138
                     /locus_tag="VGR03_11075"
     CDS             1635..5138
                     /locus_tag="VGR03_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994866.1"
                     /translation="MKTKNVGCSAFVILRVLIASILCLTAGMVALFALGSLPQRSTGG
                     GAIKLDKYPAEPPSGSSQRSAVRYRGAPQHLTPVTPVRTRKLRDTAPIDPEKVVKFYH
                     PEPIPPKPPTKSGGPAGAIQTVAGPQVSAPSPTGVSFEGVGVGLAGFSPSGNPPDVNG
                     RVGSTQYVQWNNTSFAVFNKTTGALLYGPTAGNTLFQSLGGVCASHNDGDPVVSYDIL
                     AGRWVISQFAVAGGDTSYSHQCIAVSTTEDATGEYYVYDFLTDATNFVDYPHTGMWPD
                     GYYMSAHVFSVVPTDLSPVGLPVAPPNAFIQGRIYVFERQKMIDGASARMQSMDLGQE
                     FGFLPADLDSLTPPAAGEAEFLLGPNRVLTNLTDPFRVVVTWDPAPTIAVLPGAAIMT
                     GIGNAPCVNSGRACVPEPPPAAATDYLDSIGGHYMYRLAYRNQGTQAAPDESLVLSGT
                     TSGSASSPAHGAIRWWEFRNPTPGSSTTMPTSFQDGTYDPDTSYRWIPSIAMDKDRNI
                     ALGYSKSSPTVFPGIYVTGRLATDPAGTMGAEALMQAGLGVQLGAGNRWGDYSSMTLD
                     PIDQCTFYYTNEYLKTNGGFNWSTRITSYKFPTCLSAANLYGTVTGTITSSETGGPIS
                     GVRVALSNGYAGASDVNGIYTILVPAGTYTATAADAARNCVSASPASVNVAPPGGGTV
                     TQNFIMSGTSKLDANGFTIDDSLGNGNGIVNRAECVKLNLGIKNNGCAKATAISATLT
                     TTTSGVTVLQGNSSYSDKAIDESGTNSTPFKISVSNSFSCGTPIALSLNLTYAGGSKS
                     IAFTVPTCAGGPDQTIPSNSITTSDASQPDRLGRNGVQSPCSGKHCPGAINSAGTRNY
                     KTFTFTNTSGAARCYTVTINAATGVANTGDIESVAYQTTYTPPTAMGDANGNMCMNYL
                     GDSGISGLGTTVGSASYSFTVAANSNFVIVVNTTAGSTNSSVFSGTVSGFIDNTPGPG
                     DCATIPPELTGAVSRKTHGNLTPPPAGPGDLGLNLNPSTPATIEPRMGGSPTGNHTLV
                     FRFFNTLTGPGATSITATATTSSGTQNVTASGSIGTDTHEYIVNLTGVPNASHVNVTL
                     NGVTDSVPSTGNVGPVRMDVLLGDVNATRGVDGNDVSEVQSHTRQPVTNANFRDDVNA
                     TGGIDGNDVSITQGQTRTSLP"
     gene            5680..>8409
                     /locus_tag="VGR03_11080"
     CDS             5680..>8409
                     /locus_tag="VGR03_11080"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEV1994867.1"
                     /translation="MAFFAQAGVPRTFDRKIKSQHPAQRPDVNFFSFEAPTGPTAPTT
                     TITVNSTSPVIANDGFCTLPEAIIAANTQTASGSMPGECPAGSSGGNTIVLAVGTTYN
                     LVAAHNSFYGPNGLPAVTSMIFISGNGAIIQNAGGNFRLFYISPAGNLTLQNLTVQSG
                     LAKGGNGGAGPGTGGGGLGAGGAIYNQGTLVLNGTTLSGNTAQGGFGGPTTEGGFASG
                     GGGIGGDGGSSSGLFAGGGGGGGMGAIGGSSINNGGGGGGGGQNLNENGSPSAAGTGG
                     AGGGTSGGAGGNGTPITPTSGLNGTFGGGGGGGGNAGVSGMVGGSGGNGGIGGGGGGC
                     QDAACHAGNGGFGAGGGAGGNGTGGTGGFGGGGSGGTAAHGAPGFGGGIAGGPVFTSG
                     GGGGGGMGGAIFNDGGTVNIFNSTLSGNAATGGDGSTAGQAGAGGKGFGGGLFNLNGT
                     VVIDFSTVAGNTVAAGRGGTTVSASGVYNHREGAGTATMVLRNTILGGIGAANCTNDG
                     AATFISSGYNLAQTPGTTCGFTGTGDLTGVDPMLAALAANGGPTNTQALQAGSQAIDR
                     IPNGINGCQQGVSVDQRGALRAVPGPGDVNTSCDSGAYEFNSGAVPTSTPPPGTPRFF
                     NYQSPPGTGDSAGEPSIGSNWTKETISHNHNVNGSPDNNIPNGGTSLYFGGFLPSMVK
                     VTWDDCSSPAGTLWENKPLLGASTPRAAGDPILFTDHDTGRTFVCQLEGLTPAGSTID
                     ITDDDGVNFIPSDGVIPSDVDHETIGGGRNHSPLPNPGPVYQNAIYYASQSIGEARAF
                     RSDNGGLLFSQATTPMYAINDCSGLHGHIKVSPADGTVYVPNRGCGGAVPFHETGARQ
                     TLLVSEDNGITWTQRPIPDSTTHGNGDADNIVQGTHDPSVGVATDGTVYFGSQG"
BASE COUNT         1792 a         2469 c         2507 g         1641 t
ORIGIN      
        1 caacttgctc ggcgtaaatg acggcaccac tacgcgcgac atttccgttc caatggcagt
       61 gttgatcggc gacacgacag gaaacggcgc ggtgaactcg agcgacatcg cgcaaacgca
      121 gtcgcagtcg ggtcagcttg tgagcgcgag taacttccgc gaagatgtca ccgcgaacgg
      181 ccagatcaac agctcggata tcgcactggt tcaatccaag tctgggacag ccttgccgtg
      241 aaatcggatc agctctaatt cggaggcgac acgaacgaag cgaaatcgaa ctccggcagc
      301 agcgctcttc ttaatacttg ggactgaccg cttctgggtt cccttctgcg cttccaaaat
      361 gcctccgatg agaacagatc agccgcgtat taacgttcgt cgcgccgcgc tcatctttgt
      421 cgcgggcatc attaccacat cgtgttccga aaagccggcg ccggatcggg aaaaggatgt
      481 gaacgcgtcc gaagcgccac gcgagatcgt gcgcgagtcc gaagtggaaa cggctgaagg
      541 cggagacgag ccccagcccg gcaaagatga gatcgaggaa gattgcgccg cgttcgttcg
      601 ctccaccaaa gttgtccctg ctcgggcagc ggccaccgat tgcccgggat gtcccgccgg
      661 agggacggcg gtcctggcct ttcgcagcat gaaaacggat gtggtctcct gctccggcga
      721 tacgtgcacc gtggtggtga cgattcgcgc ggtgttcaat cccggttcgg gtgaaacagt
      781 tactggagga ctgactgcct ggattcctgc ggaacagcga aacgcatatt tgagtgggca
      841 cacgccatcg gacgaacagg tctatcgcgt acgaattacg tacaagcgga gcggcgcggc
      901 atggcgagcg gtcgaattcg atcgcgcgcc tggcgaatga gtccgagcta accacggcga
      961 gtccgtccga tcggcggacc ggacgatcgg acaatgttct cgtgtttatc agccggcgcg
     1021 cgatatatat atataagaga tggctcacgc gtcggtttcc agtatcgcag cgacgcttat
     1081 cgcgctcgcc tgcatgacag ccgtggcaga aacaccggct cccgggacga gcgggattga
     1141 aggcatgatc gttgtctcgc cgacccgtcc cggaccgatc aggaaggatg aggagctgag
     1201 tgtcgcgccg gtgagtaacg cgcagttcgt ggtcaaagcc ggcgatgcga ctgtcaagac
     1261 attcacgacg gatggcgacg gtcgcttcca agtcgggtta ccacctggtc attacgtgat
     1321 cctgagagaa ggggccgcac ccagagttgg tcgctggcgt ttcgaggcgg atgtcgtcgc
     1381 cggccagatg accaaggtga actggacggc cgacagcggc atgcgctgaa cggtaaccga
     1441 tcggcatttt cttgagttcg gacgtacgaa gctcccactt gggctcattc gagagcaaaa
     1501 gttttgacgc gttgttcaga aaatgataca accggatgct tgcccgaaaa cgaagagaag
     1561 aagttcggcg tgccacatga ccggcaggtg atcggcgggt tccggtttgt gactcgtctc
     1621 cggaaatcct aactatgaaa acaaagaatg tcggctgctc agctttcgtc atcttgcgcg
     1681 tgcttatcgc atctatcctg tgcctcactg cggggatggt cgctctgttc gccttgggat
     1741 cgctcccgca gcgctctact ggcggtggcg cgatcaaatt ggacaagtat ccggccgagc
     1801 cgccgtcggg tagttcgcaa cgctcggcgg ttcggtatag gggcgcgcca cagcatctga
     1861 ctcccgtcac cccggttcgc acgcgaaagt tgcgcgacac cgcgccaatc gatccggaga
     1921 aggtcgtgaa attttatcat ccggaaccga tccctccgaa gcctccgacg aagagcggcg
     1981 gaccggccgg agcgattcag accgtagccg gaccgcaagt gtcggccccg agtcctaccg
     2041 gtgttagctt cgaaggtgtc ggcgttgggc tggctggatt cagcccttcc ggtaatccgc
     2101 cagacgtgaa cggtcgggtt ggctcgacac aatacgtgca atggaacaac accagcttcg
     2161 ccgtcttcaa caaaaccacg ggcgcccttc tgtacggacc cactgccggt aatacgctct
     2221 ttcaatcact gggcggcgtt tgcgcgtcac acaatgacgg cgacccggtc gtctcttacg
     2281 acattctcgc ggggcgctgg gtgatctcac agtttgctgt ggctggcggg gacaccagct
     2341 attcacacca gtgcatcgct gtttcgacga cagaagacgc gacgggagag tattacgtgt
     2401 acgacttcct gactgacgca accaacttcg tcgattaccc gcacaccggc atgtggccgg
     2461 atggctatta catgagcgcg catgtgttta gcgtagtgcc aactgatctc agccctgtcg
     2521 ggctgccggt cgcgccgccg aatgcgttca tccagggccg catttatgtc ttcgagcggc
     2581 agaagatgat cgacggggct tccgctcgca tgcaaagcat ggatctcgga caggaattcg
     2641 gattccttcc ggctgatctc gacagtttga ctcctccggc ggctggcgag gcagagtttc
     2701 tcctcggccc aaaccgggtc cttaccaacc ttactgaccc gttccgagtg gtagtgacat
     2761 gggatccagc tccaacgatc gctgtgctac cgggtgcagc aatcatgacc ggcatcggca
     2821 acgcaccgtg cgtcaattcg gggcgcgctt gcgtgccaga gcctccacca gccgctgcaa
     2881 cggactatct cgatagcatt ggcgggcact acatgtatcg gctggcttat cggaaccagg
     2941 gcacgcaagc cgcgccggac gagtcgctcg ttctcagcgg cacgactagt ggcagcgcaa
     3001 gcagtccggc acacggagcg ataagatggt gggaattccg gaacccaact ccggggagct
     3061 cgacgacaat gccaacatcg ttccaagacg gcacctacga tccagatacc agctaccgtt
     3121 ggataccgtc catcgccatg gacaaagatc gcaacatcgc gctgggctac agcaagtcga
     3181 gtccgacggt ttttcccggc atctacgtca ccggccgact ggctactgat cctgccggca
     3241 caatgggtgc cgaggcattg atgcaggcag gccttggtgt gcagttaggc gccggaaatc
     3301 gctggggtga ctatagctcg atgacactgg acccaatcga ccagtgcacc ttctattata
     3361 cgaacgagta cttgaagacg aacggtggct tcaactggtc cacccgaatt acgagctaca
     3421 agtttcctac ctgcctctcg gccgcgaatt tgtacggcac ggtcaccggc acaatcactt
     3481 catctgaaac aggtggtccg atatccgggg tcagggtggc gttgagcaac ggctacgcgg
     3541 gcgcgagcga cgtaaatggc atctacacca ttctcgtccc ggctggcacc tacaccgcta
     3601 ccgccgcaga cgcggcgcgc aactgcgtgt cggcgtcgcc tgcttcggtg aacgtcgcac
     3661 cgccaggcgg aggcacggtc acgcaaaatt tcatcatgag cggcacatcg aagctcgatg
     3721 cgaacggctt cactatcgac gactcgttag gcaacggcaa cggcatcgta aaccgagccg
     3781 aatgcgtgaa actgaacctc ggcatcaaga ataacggctg cgcgaaagca acggcaatat
     3841 ctgccaccct aacgacaaca acttcgggcg tcacggtttt gcagggcaac tccagctatt
     3901 cagataaggc gatcgacgag agcggcacga actcaacgcc gttcaagatc tccgtctcca
     3961 attcattcag ctgcggcacc ccgatcgcgc tttcacttaa cctgacttat gcgggcggat
     4021 cgaagtcgat cgcattcacg gtgccgacct gcgctggcgg ccccgaccaa acaattccat
     4081 cgaactccat tacgacgagc gatgcgtctc agccggatcg tctgggtcgc aatggcgtcc
     4141 agagcccttg ctcaggaaag cattgccccg gggcgattaa ctccgccggc acccgcaatt
     4201 acaagacatt caccttcact aacacgagcg gcgcagctcg ttgctacacc gtcacgatca
     4261 acgcggccac cggtgtagcc aataccggtg acattgagag cgtggcgtat cagaccacat
     4321 atactccgcc gacagccatg ggcgatgcaa acggaaatat gtgcatgaat tacctcggtg
     4381 acagcggaat cagcggcttg ggaactaccg tagggagcgc tagctactcg tttaccgtcg
     4441 cggcgaattc gaatttcgtc atcgtcgtga acaccaccgc tggctcgacg aattcgtcgg
     4501 tattcagcgg gaccgtgtcg ggattcatcg acaacacgcc ggggccgggc gactgcgcga
     4561 cgataccgcc ggagcttacc ggagcagtat cgcggaagac acacggcaac ctgactccgc
     4621 cgccggcggg accaggggat ctggggctta acctcaaccc gagcactccc gccacgatcg
     4681 agccgcgcat gggcggtagt cccacgggaa atcacactct ggtgtttaga ttcttcaata
     4741 cgctgactgg tcctggcgcc accagcatca cggccacggc caccactagt agcggcacgc
     4801 agaacgtgac cgcgagtggc agcatcggga ctgacacgca cgagtacatt gtcaatctca
     4861 ccggggttcc caatgcctcg catgtgaacg tcaccctcaa tggtgtaacc gattccgtcc
     4921 ccagtactgg caatgttggc ccggtacgga tggatgtgct cctcggcgac gtgaacgcca
     4981 ctcgtggtgt cgatggaaat gatgtctcgg aggttcagag tcacacccgg cagccggtaa
     5041 ccaacgcgaa cttccgtgat gatgtcaatg ccactggcgg cattgacggc aacgatgtgt
     5101 caataacaca aggccaaacc cgaacctcgt tgccttgagt tccataattc aggcttacct
     5161 ccgttaaacc taacgctaac gggctggcca tttcggtcag cccgtcagcg gttgtaagcg
     5221 attgattaaa gcctcttcgg cgagcttctt tgacttgtcg atcttagccg cctgatcgcc
     5281 agcaccgccg acgcttattc taacaacatt gtttcagcac ggtttccacc actcttgggg
     5341 atcgcgcagg aaatttaaag ctgcctaacg aacagcattc gtttcgcgca caacgtcgcg
     5401 gccaaagcca acggattcgt gagttctctt tctgaattag ccgcgcgcag caacagtcaa
     5461 aagagagttc tcgccatcgc cagagcggag cttgcccaaa aaattttcgg aatcgagccg
     5521 aggcatccat acgcactgct gattacgatt cttgtcttcg aaggtaatga attccacctg
     5581 caatccgaga aatctatgaa aaagaaactc atctcccaat ccggcctttt tagtttccgc
     5641 gttttattag cttttagtct ttgttctgtt ggcgtcctct tggctttctt tgcgcaagct
     5701 ggcgtacctc ggactttcga tcgaaaaatc aaatcgcaac atccggccca gcggccggac
     5761 gtcaatttct ttagtttcga ggcgccaaca gggccaacag cgccgactac cacgatcacg
     5821 gttaacagca cgtcgcccgt gatcgccaat gacggctttt gtaccctgcc ggaagcgatc
     5881 atcgcggcta acacccagac cgcctccggt tcgatgccgg gcgaatgtcc cgcgggcagc
     5941 agcggcggca ataccatcgt gctggcagtc ggcaccactt acaacctggt ggccgcgcat
     6001 aactccttct atggacccaa cggtctgccc gccgtcacca gcatgatatt catctcgggc
     6061 aatggagcaa tcattcagaa tgccggcggg aacttccgcc tcttttacat ctcgcccgcc
     6121 ggcaatctca ccttgcaaaa cctgacggtg cagagtggcc tggcaaaggg aggaaatggt
     6181 ggagctggac ccggcaccgg cggcggcgga ttgggcgcgg gcggtgccat ctacaaccaa
     6241 ggtacgctcg tgctgaatgg caccaccctt tccggcaata cggcgcaggg cggctttggc
     6301 ggacctacca cagaaggagg atttgccagc ggcggcggtg gaataggcgg cgacggcggc
     6361 tctagcagcg gcctttttgc gggcggcgga ggcgggggcg ggatgggagc gattggcggc
     6421 agctccatta ataacggagg cggtggcggt ggtggaggtc aaaaccttaa cgaaaatggt
     6481 tctccctctg cagccggtac tggcggtgcg ggcggcggca catccggagg ggccgggggg
     6541 aacgggactc ccattactcc cactagcggt ttgaacggca cgtttggtgg tggtggtggt
     6601 ggtggtggca acgcgggcgt gtcgggcatg gtgggcggca gcggcggcaa tggaggaatt
     6661 ggcggcggtg gaggcgggtg ccaggatgcc gcttgccacg ctggcaatgg tggttttggg
     6721 gctgggggtg gtgctggtgg taacgggaca ggtggcacag gtgggtttgg aggaggggga
     6781 agcggtggca ccgctgcaca cggcgcacct ggctttggtg gaggaattgc cggtggtccc
     6841 gtattcacta gtggaggcgg aggcgggggc ggtatgggcg gcgcgatctt taacgacggc
     6901 ggaacagtga acattttcaa cagcacactt tccggaaacg ctgccactgg cggcgatgga
     6961 agcacggcag ggcaggctgg cgccggcggc aagggcttcg gcggcggact cttcaaccta
     7021 aatggcacag tcgtcattga ctttagcacc gtcgcgggca acaccgtcgc ggctggtcgt
     7081 ggtggcacaa cggtcagcgc cagcggggtc tataaccacc gagagggcgc cggcacagcg
     7141 acgatggttc tgcggaacac catcttgggc ggaataggcg ccgccaactg caccaacgat
     7201 ggcgccgcca ccttcatctc gagcggctat aacctggcgc agacgccagg cacgacatgc
     7261 ggctttactg ggactggcga cctgaccggc gttgacccga tgctcgccgc gctggctgct
     7321 aatggcggtc cgaccaacac gcaggcgctc caggctggga gccaggccat tgacagaatt
     7381 ccgaacggaa tcaacggctg ccagcaaggg gtaagcgttg accaacgcgg cgcgctgcgc
     7441 gccgtgcccg ggcccggtga cgttaacacg tcctgtgaca gcggcgcgta cgagtttaat
     7501 tccggagccg taccaacgtc gacaccgcct ccggggacac cgagattctt caactatcaa
     7561 tcgccaccgg gcactggtga cagcgccggt gaaccttcca tcggcagcaa ttggacgaag
     7621 gaaaccatat ctcacaacca taacgtcaat ggttcgccgg acaataatat cccgaatggc
     7681 ggaacgtcgc tctattttgg cggatttctg ccgtcgatgg tgaaagtcac gtgggacgac
     7741 tgctcttcgc ccgccggaac actttgggag aataagccgc tgttgggcgc gagcacaccg
     7801 cgagccgcgg gcgacccgat cctgttcacc gatcacgaca cgggacgcac gttcgtttgt
     7861 caattagagg ggctcacgcc ggctggaagt acaatcgaca tcaccgatga tgatggtgtc
     7921 aactttattc caagtgacgg cgtcattcct tccgacgtgg accatgaaac gattggcggc
     7981 ggccgtaacc attcacccct cccgaaccca ggtcccgttt atcaaaatgc aatctattat
     8041 gcctcgcaga gcattgggga agcgcgagcc ttccgcagcg acaatggtgg attacttttc
     8101 agtcaggcta ctacgccgat gtatgcgatc aatgattgtt cggggctgca cggccacatc
     8161 aaggtatcgc ccgccgacgg cactgtctat gtgccgaaca gaggatgtgg cggcgcggtc
     8221 ccattccacg aaaccggcgc caggcagacg ctgctcgtct cggaggacaa tggcatcaca
     8281 tggacgcaac gaccgattcc ggactctacc actcacggta acggtgatgc tgataatatt
     8341 gtacagggca ctcacgatcc ctccgtcggt gttgccactg atggcaccgt ctattttggc
     8401 tctcagggc
//
LOCUS       DASYVO010000327         4708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_232611, whole genome shotgun sequence.
ACCESSION   DASYVO010000327 DASYVO010000000
VERSION     DASYVO010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232611"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..757
                     /locus_tag="VGR03_11085"
     CDS             161..757
                     /locus_tag="VGR03_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994868.1"
                     /translation="MQVREAKDFLVKQAVEQARVEDVPLSDLERRMMYFTEGPDAVED
                     PVSLNEEFEKQYDTTQYEAKLTKLLANAHKRLKTESPISISEWDKAIETLQEGDHYLL
                     VLSGTGSLGRIPLRSLLTLAARLAVLIFAASVVWIGLKYFLKATGTSAGSIFAVVFVA
                     SLLVAAIKPQAVSDLSRRGLMWVILFLFGRRDSSGHGN"
     gene            complement(761..2785)
                     /gene="ligA"
                     /locus_tag="VGR03_11090"
     CDS             complement(761..2785)
                     /gene="ligA"
                     /locus_tag="VGR03_11090"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV1994869.1"
                     /translation="MAKAAPAAVRKEIEVLREKLRYHEYRYYVLDDPEISDAAYDRLM
                     NRLRELEVAHPELVTPDSLTVRVGGAPREGFSTVRHARAMLSLDNAFSYDALREFDRR
                     VREGSGQEKIEYIAEHKFDGLSISLQYVDGVLARGATRGDGTTGEDVTPNVKTIRSIP
                     LRVDAGVLKKIKLPASFEVRGEIMMTRKAFEALNRQQEQTGGKIFVNPRSAAAGAVRV
                     LDPAITAARRLDFFAYYLLVDGKVPFAKHSESLQALKQLHFRASNDWKLCAGIEAVTE
                     YCDDWDEKREKLAYEIDGVVVKVNAIPIQNELGYTSKAPRWAIAYKYPARQETTVVND
                     IIVQVGRTGTLTPVAVLEPVQVGGVTVSRSTLHNLDEIERLGLQIGDTVLIERAGEVI
                     PHVLKVTKEGKSRKPFRMPKHCPECDSAIHHVEGEVAYRCVNAACPAKRKESILHFAG
                     RHAMNIDGLGDKIVDQLVDKGLVKDVADLYALKEDAVAELERMAEKSAQNLLEEIEAS
                     KKNSLERLIYALGIQFVGERTGQLLAEHFSSLEELAAAEEEQLVEVPEVGPKVAASIV
                     EFFSEAANRQLIKKLNKAGVHPTAEKRKVKSDKFAGKSFVFTGGLVNRSREEAGELVQ
                     QHGGKVSGSVSKKTDYVVVGTDPGSKYDKAKELGVTILSEAEFERLLGLK"
     gene            2878..3831
                     /locus_tag="VGR03_11095"
     CDS             2878..3831
                     /locus_tag="VGR03_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEV1994870.1"
                     /translation="MITDLRNALLIHNPNAGNGGTGRRRTLEAARRILAAGGIETELA
                     ETTGPGHATEIALRAAAEGRGLVIACGGDGTLNEVINGLAAHHNGHRVPLALLPGGTA
                     NILAKELDLPWDIPSAAEKLVRGTVKEIALGLATPLEQPEKKKYFLSVGGAGPDGMIV
                     YSLDLDLKARLGMLAYWWQGTREVFRYNFQHFRIVIGDQKLDASLVIVGRTKNYGGPF
                     KITTGAELFEDQFEVMALTTQSGLRYLSYLPTLWMGNLRGTKGVHFYKSNSVICEPLD
                     KNPLYAQVDGEPLARLPVEFKIVPRALKLLVPANPSKPSPL"
     gene            4034..>4708
                     /locus_tag="VGR03_11100"
     CDS             4034..>4708
                     /locus_tag="VGR03_11100"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEV1994871.1"
                     /translation="MDPLTHALASYTLKRAAFPRVPRSTAIAMLVAGTIADIDSLSKF
                     AGPSAFLTFYRTYCHSLLAALFFSLLVTLPFLLRKRGPTENPSPLPIFLAALAASLLH
                     LLMDLCQNTGVEVFWPLSPRRFALDWLPHLDLWILGILLAGILLPTLSRLVTEEIGAK
                     SKGPRGKVGASLALATMILYIVLRFVLHGNALAAMESRTYRGESPRRAAAFAESGSPF
                     RWRGIVE"
BASE COUNT          934 a         1586 c         1131 g         1057 t
ORIGIN      
        1 agcgtgcctc aggacggtgt ccggactttc ctcccgctca gccatcttgc gatgaccaag
       61 ccagcgatca cccggcccgc ccgccatctt tgattataca ccgtgcgttc gaggaaaatt
      121 gggcttgttg tcctctgtca cccgacctag aatctcgcgc atgcaagtac gcgaagccaa
      181 ggatttccta gtaaagcaag ctgtcgagca agctagggtc gaggacgttc cactctccga
      241 cttggagaga cggatgatgt atttcaccga gggacctgac gccgttgaag atcccgtttc
      301 gctgaatgaa gaatttgaga aacagtacga cacaacccag tatgaagcca agctgacaaa
      361 gttgctcgcg aacgcgcaca agcgcctcaa aacggagagt cccatatcga tcagcgaatg
      421 ggataaggcg atcgagacgc tccaagaagg cgatcattac ctgctggtgc tttcggggac
      481 cggatctctg gggcggatcc ctcttagaag cttactaacg ttggcggctc gattggcagt
      541 gcttatcttt gcggcttcag tagtgtggat agggttgaag tatttcttga aggcaaccgg
      601 aacttccgct ggatcaattt ttgcagtggt cttcgtagcc agcctcctcg tagcggcaat
      661 aaaaccacag gcggtaagcg atctttcaag gcgcggctta atgtgggtca ttctattctt
      721 gtttgggcgc agagactcaa gcggccacgg gaactagaga tcatttcaat ccgagaagcc
      781 tttcaaattc cgcttcgctg aggatggtga ctcctaattc ctttgctttg tcgtattttg
      841 atcccggatc ggtgccaaca acgacatagt cggttttctt gctgacagag ccggaaactt
      901 tccctccgtg ctgctggaca agctccccag cctcttcgcg gcttcgattc acgaggcccc
      961 ctgtaaagac aaaactcttg cccgcaaatt tgtcgctctt gaccttgcgt ttttccgccg
     1021 tcggatgcac tcccgctttg ttcagcttct tgatgagttg gcgattggcg gcctccgaaa
     1081 aaaattccac gatgctcgcc gcgactttcg gcccaacctc gggcacttcc acaagctgct
     1141 cttcctccgc cgccgccaat tcctccaacg aagaaaaatg ttccgccagc aattgcccag
     1201 tccgttcgcc cacaaactgt atgcccagcg cgtaaatcag ccgctccagc gaattcttct
     1261 tgctcgcctc aatttcctcc agcaaattct gcgccgattt ttccgccatg cgctccagct
     1321 cggccaccgc gtcttccttc agcgcataaa gatccgcgac atccttgacg agtcccttgt
     1381 ccacaagctg atccacaatc ttgtcgccca gcccatcgat gttcatcgcg tgccgtcccg
     1441 caaagtgcag aatcgattct ttcctcttcg ccggacacgc cgcattcacg cagcggtacg
     1501 ccacttcccc ttccacgtga tgaatcgcgc tgtcgcattc cgggcaatgt ttcggcatgc
     1561 gaaacggctt ccgcgacttc ccttctttcg tcactttcag cacatgcggg atcacttccc
     1621 ccgcccgctc gatcagcacc gtgtcgccaa tctgcaaccc cagccgctcg atttcatcca
     1681 gattgtggag cgtggaacgg ctgacagtga cgcctcccac ttgcaccggc tccagcaccg
     1741 ccacaggcgt cagcgtcccc gtgcgcccca cttgcacgat gatgtcgttg accaccgtcg
     1801 tctcttgccg cgccggatat ttgtacgcaa tggcccaccg cggcgctttg gacgtgtaac
     1861 ccagttcatt ttgaatggga atggcgttta ctttcacgac cacgccgtca atctcgtacg
     1921 cgagcttctc tcgcttctcg tcccaatcgt cgcaatactc cgtcaccgct tcgattcccg
     1981 cgcaaagttt ccaatcgttc gaagcgcgga agtgtaattg cttcaaagcc tgtagggact
     2041 ccgaatgttt cgcaaacggc acttttccat ccacgagcag gtagtacgca aagaagtcca
     2101 gccgccgcgc cgccgtaatc gctggatcga gcacgcgcac agccccggcc gctgcgcttc
     2161 gcggattcac gaagattttt ccgccggtct gctcctgctg ccggttcagc gcttcgaacg
     2221 ccttccgcgt catcatgatt tcgccgcgta cttcgaaact cgctgggagc ttgattttct
     2281 tcaacacccc tgcgtctacg cgcagcggga tcgaccgaat cgtcttcacg ttcggcgtga
     2341 catcttctcc cgtcgtgcca tcgccacggg tcgctccccg cgccagcacc ccatccacat
     2401 attgcagcga gatgctcaac ccatcgaact tgtgctccgc gatgtactcg atcttttcct
     2461 gcccgcttcc ttcccgcacc cggcggtcga actcacgcaa cgcatcgtaa gagaatgcat
     2521 tatccaggct gagcattgcc cgcgcgtgcc gcaccgtgga aaatccttcg cgcggcgcac
     2581 cacccacgcg caccgtcagc gaatccggcg tcaccagttc aggatgcgcc acctcgagct
     2641 ccctcaaccg gttcatcagc cggtcgtagg ccgcgtccga aatctccgga tcatcgagca
     2701 cgtaataccg gtattcgtgg tagcgcagtt tctcacgaag cacttcgatc tctttcctga
     2761 cggcagcggg tgcagccttg gccatgaaac ccctcggcag aacaatgtcc tggaattcgg
     2821 atggtattat atagaatgaa tgtcttggag cacgaacggt gtggcactga acctgtgatg
     2881 atcactgatc tccgaaacgc tttgctgatt cacaacccca atgccggcaa cggcggcaca
     2941 ggccgccgcc gcacgctcga agcggcgcgc cgtattcttg ccgctggcgg catcgagacc
     3001 gaacttgcgg aaaccaccgg cccgggccac gccacagaaa tcgcgcttcg cgccgccgcc
     3061 gaaggccgcg gcctcgtgat cgcttgcggt ggcgacggca ctctcaatga agtcatcaac
     3121 ggcctcgcag cacaccacaa cggccaccgc gtgcctctcg cccttcttcc cgggggaacc
     3181 gcgaacatcc ttgccaagga gctggacttg ccctgggaca ttcccagcgc cgccgaaaag
     3241 ctggttcgcg gcacggtgaa ggaaatcgcg ctcggcctgg caacgccgct cgaacaaccc
     3301 gaaaagaaaa agtattttct cagcgttggc ggcgccggtc ccgacggcat gatcgtctat
     3361 tccctcgacc tcgacctgaa agcgcgcctt ggcatgctgg catactggtg gcagggcacc
     3421 cgcgaggtct tccgttacaa tttccagcat ttccgcattg tcatcggcga tcaaaaactc
     3481 gacgcttctt tggtgattgt cggccgcacc aagaattacg gtggcccctt caaaatcacc
     3541 accggcgcgg aactcttcga agatcagttc gaagtcatgg ccctcaccac gcaaagcggc
     3601 ctgcgctatt tgagctactt gcccacactc tggatgggca acctccgcgg caccaagggc
     3661 gtgcacttct ataaatccaa ttccgtcatc tgcgaaccgc tcgacaaaaa tcccctctac
     3721 gcccaggtcg acggcgagcc tctagcccgc ctccccgtcg aattcaaaat cgtcccccgc
     3781 gccctgaaac tcctcgtccc cgccaatcct tcgaaaccgt cgccgttata accgcctgtg
     3841 caggggcgaa cttcaattcg ccctgttttt gtcttcgtag gggcgcagca tgctgcgccc
     3901 catcttggca accaccgcga aattcgagag ttatttatcc aatattttct gtgacgccgc
     3961 gccttccgag aaaatcagga ctaaaaactt acaactaatc actaaaaact attttcatga
     4021 gataacctta cccatggatc ccctgaccca cgccctagcc tcctacaccc tgaagcgcgc
     4081 cgctttcccc cgcgtccctc gctccaccgc catcgccatg ctcgtcgcag gaaccatcgc
     4141 cgatatcgac tcgctcagca aatttgccgg accctccgcc ttcctcacct tttaccgcac
     4201 gtattgtcat tccctgctcg cggctctctt cttcagtctg ctagtcactc tcccctttct
     4261 cctacgcaag cgcggaccaa cggagaatcc ttcgccgctt ccgatcttcc tcgccgctct
     4321 cgccgcctcc cttctccatt tgctcatgga tctctgccaa aacacgggcg tggaagtctt
     4381 ctggcccctc tccccgcgcc gattcgctct cgactggctc ccccatctcg atctctggat
     4441 tttgggaatc ctccttgccg gaatccttct ccccacgctc agccgcctgg tcaccgagga
     4501 aatcggcgcc aaatcaaaag gcccgcgagg caaagtcgga gcgtccctcg cactcgccac
     4561 catgattctt tatatcgtct tgcgcttcgt tctccatggc aacgctttgg ccgccatgga
     4621 atcgcgcacc taccgcggcg aatcgcctcg cagggctgcc gcgtttgccg aatccggctc
     4681 gcccttccgc tggcgcggca tcgtcgaa
//
LOCUS       DASYVO010000328         3103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_206049, whole genome shotgun sequence.
ACCESSION   DASYVO010000328 DASYVO010000000
VERSION     DASYVO010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206049"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1664
                     /gene="glmS"
                     /locus_tag="VGR03_11105"
     CDS             <1..1664
                     /gene="glmS"
                     /locus_tag="VGR03_11105"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV1994872.1"
                     /translation="GIGHTRWATHGRPTEENAHPHRDCTGQIVVVHNGIIENYLELKE
                     KLQKEGHKFATETDTEIVAHLVEKNSQGGVPLEEAVRLSLKELRGIYALVFLSAKDPQ
                     KIVAARLGPPSVIGLGDGEYFVASDIPALLQHTREMFFLADGDIAVLTAKGVHVMDHD
                     GRPVERPAHHVAWDPIMAEKGGYKHFMQKEIFEQPRAVRDTLLGRISQDTGKVFLDEM
                     AITEKQFREFQQVRIVACGTSWHAGLAAKFMIERLARLPVEVDYGSEFRYRDPILDLK
                     TLTVCISQSGETADTLAAQREAKSKGSPSLAICNVIGSMITREANGTIPTHAGPEIGV
                     ASTKAFTAQLTALLLLASYLAQVRGKMSEDAARTLMQEFTRIPHKIETVLQADESGFY
                     ENLARQFFRYTDFLYLGRGIHYPIALEGALKLKEISYIHAEGYPAGEMKHGPNALIDE
                     NLPVVVLATRDESDPEAMTRYEKSVSNIKEVKARDGIVISIVTTGDHLAREASDHIIE
                     LPPAPELLVPLLEIIPLQLLAYHIAVRLGCDVDQPRNLAKSVTVE"
     gene            1693..3006
                     /locus_tag="VGR03_11110"
     CDS             1693..3006
                     /locus_tag="VGR03_11110"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEV1994873.1"
                     /translation="MLRNFRACLGTLSLLLLPAGFPSSAQTPDAATAPLREVHADGEK
                     LLTEAQVIAITSLIPGAQIGKNDLQTAADRLVQSGLFAKVSYNFQTKVAGVLVTYHVE
                     ESPRIPVYFDNIPWSTDSALGDAIRTQFPFFDGTLPEAGAAVDQAGEAVKDLLSTRGL
                     HVSLEHTVIANPNGEGNVQEFRIEGAALQIAKLEFSDPTLLASKAVQQHLSEILGKPY
                     SRMTIDLFLSEAIKPIYLQQGFLRAKLGPPEVRLTGDPNQKLPSQIPVYVPVAPGEIY
                     RWKEIHWTGNTLVSEFTLNGLLGVKHGDVADGMQIEAAWDRAREEYAHRGYLEAKLDP
                     APAYDDQAHTVSYSVSIQEGPQYRFGKMVLTGISPAAERKLYAAWPFAAGEIFDKAKF
                     EELLAKLQTHQEQVFGELPVHYENVGHWLQTDASKSTVDVLLDFK"
BASE COUNT          701 a          946 c          851 g          605 t
ORIGIN      
        1 acggcatcgg acacacgcgc tgggccaccc acggacgccc caccgaagaa aacgcccacc
       61 cgcatcgcga ctgcactggc caaatcgttg ttgtacataa cggaatcata gaaaactacc
      121 tcgagctgaa ggaaaaactg cagaaggaag gccacaagtt cgccacggag accgacacgg
      181 agattgtggc gcacctcgtg gaaaaaaatt cgcagggcgg cgttcccctc gaggaagcgg
      241 tgcgcctctc gttgaaggaa ctgcgcggca tctacgcgct ggttttcctt tccgcgaaag
      301 atcctcagaa gattgttgcg gcgcgcctgg gcccgccgtc ggtgattgga ctcggcgatg
      361 gagaatattt cgtcgccagc gacatccctg ccctgctcca gcacacgcgc gaaatgtttt
      421 tcctggcgga cggcgacatt gccgttctca ctgcaaaagg tgtgcatgtg atggaccacg
      481 acggccgccc tgtcgaacgc cctgcgcacc acgtcgcctg ggaccccatc atggcggaaa
      541 agggcggcta caagcacttc atgcagaagg aaatttttga gcagccgcgc gccgtgcgcg
      601 ataccctgct cggccgcatc tcgcaggaca ccggaaaagt cttcctggat gagatggcga
      661 ttacggaaaa gcagttccgc gaatttcaac aggtgcgcat tgtcgcctgc ggcacgagct
      721 ggcacgccgg gctggccgca aaattcatga ttgagcgcct cgcccgtctt ccggtcgaag
      781 tcgattacgg aagcgaattc cgctatcgcg accctatcct ggatttgaaa acgctgaccg
      841 tgtgcatcag ccaatccggc gaaaccgcgg acacgctggc agcccagcgc gaagcgaaat
      901 cgaagggctc gccctcgctg gcgatctgca acgtgatcgg ttccatgatc acccgcgaag
      961 ccaacggtac gattccgacg cacgccgggc cggaaatcgg cgtggcttcc accaaagctt
     1021 tcactgcaca attaaccgcg ctgctcctgc tcgcttccta tctcgcccaa gtgcgcggca
     1081 agatgagcga ggacgcagcc agaactttga tgcaggaatt cacccgcatc ccccacaaaa
     1141 tcgaaactgt tctgcaagcg gatgagtcgg gattttacga aaacctggcg cggcagttct
     1201 ttcgctacac agattttcta tatctcggca ggggcatcca ctatcccatc gcgctcgaag
     1261 gcgcgctgaa gctcaaggaa atttcctaca ttcatgcgga aggctatccc gcgggcgaaa
     1321 tgaagcacgg ccccaacgct ttgatcgacg aaaatctgcc ggtcgtagtc ttggcgacgc
     1381 gtgacgaatc cgacccggaa gcaatgacgc gctacgagaa aagtgtttcg aacatcaagg
     1441 aagtcaaagc gcgcgacggc atcgtcatct ctattgtcac cacgggagat catctcgcgc
     1501 gtgaagcctc cgatcacatc atcgaacttc cacccgcgcc ggagttgctc gtgccgctgc
     1561 tggaaatcat ccctctccaa ttgctcgctt atcatatcgc cgtgcgtctc ggttgcgacg
     1621 tcgaccagcc gcgcaacctg gcgaaatccg tcaccgtgga gtagccgggc ccgtttccgc
     1681 tactatagga acatgcttcg caattttcga gcgtgtctcg gcaccctctc gcttctcctg
     1741 ctgccggccg gcttcccttc ttcggcgcag actccggacg ccgccaccgc gccgttgcgc
     1801 gaagtccacg cggacggcga gaaactcctc accgaagccc aggtcatcgc cataaccagc
     1861 ctgattcctg gtgcccagat cggcaagaat gatttgcaaa ccgctgccga caggctcgtt
     1921 caaagcgggt tattcgccaa agtaagttac aactttcaga cgaaggtcgc cggcgtcctg
     1981 gtgacctatc acgtggagga atcgcctcga atccccgtct acttcgacaa tattccatgg
     2041 tccaccgatt cagcgctcgg cgatgccatc cgcactcaat ttcctttctt cgacggcacg
     2101 ctacccgaag ccggagccgc ggtggatcag gccggcgaag ctgtgaagga cttgctctcg
     2161 acgcgcgggc tgcacgtgtc gcttgagcac acggtgatcg cgaatccgaa cggtgaaggc
     2221 aacgtgcagg aatttcggat cgaaggcgcc gcactccaaa tcgcaaagct ggaattcagc
     2281 gatcccaccc ttttggcctc caaagccgta cagcaacatc tatccgagat actgggaaag
     2341 ccctattcgc gaatgaccat tgatcttttc ctctccgaag cgattaagcc catttatctc
     2401 cagcagggat tcctgcgcgc gaagttgggg ccgccggagg tgcgattgac gggcgatccc
     2461 aatcagaagc tgccctcgca aattccagtc tatgttccgg tagcgccggg cgagatctat
     2521 cgctggaagg aaattcattg gacgggaaac actctggtat cggaattcac gctgaacggc
     2581 ctcctcgggg tcaagcacgg ggatgtcgcc gatggcatgc agatcgaagc agcctgggat
     2641 cgcgctcgcg aagaatatgc tcatcgcggc tacctcgaag cgaaactcga tccggctcca
     2701 gcgtatgacg atcaggcgca caccgtttcc tactcggtga gcattcaaga gggaccgcaa
     2761 taccgcttcg gcaaaatggt cctcaccgga atttctccag ctgccgagcg aaagctatac
     2821 gccgcgtggc cgttcgctgc cggtgaaatc ttcgacaaag caaagtttga ggaactcctc
     2881 gcaaagctgc aaacgcacca ggaacaggtt ttcggtgagt tgccggtgca ctacgagaac
     2941 gtcggccatt ggctgcaaac ggatgcaagc aagagcaccg ttgatgtgct tctggatttc
     3001 aagtgactcc gaatgccgcg caatctgcga tgcaagcggc actctgagat atactctgca
     3061 ctgcgatgaa agagctcatt catattcgtc atgcggaggg agc
//
LOCUS       DASYVO010000329        18141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_166642, whole genome shotgun sequence.
ACCESSION   DASYVO010000329 DASYVO010000000
VERSION     DASYVO010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 18141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18141
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166642"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..513)
                     /locus_tag="VGR03_11115"
     CDS             complement(130..513)
                     /locus_tag="VGR03_11115"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEV1994874.1"
                     /translation="MKPALGKAVGDALRKRCPNCRRGRVLARWPNKMLPRCPVCGLSF
                     FRESGYYIGGMMITYGLTAIVLIAVYLVSLLLPDTQLSVNRILALWIAFGAALSLVFM
                     PYAYSLWLSLDFWLNPWEGEGEGES"
     gene            957..1916
                     /locus_tag="VGR03_11120"
     CDS             957..1916
                     /locus_tag="VGR03_11120"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEV1994875.1"
                     /translation="MKVFAVLPDGSSGPEGLEVDSAGNVYVTTFGFTAKGSASGPGQL
                     FVFNAHGDLLRQLSIAGSSPHLLGLHFHPLTGALLVCDFGAGKVLDVDPRTGAAVVFM
                     TLPALPHPSLGAGLNDVTFDAAGNVYVSDSFQGIVWQTGPHGGVGTVWVDDPLLRTTG
                     VPPFGANGLRFNKEETALFVANTGNDTIVKVSVALNAAGQRTAAVFTNSIGGADGVIL
                     DDKNNLWVAANQSDEIVVVDPTGRVIEKLGDFAGVNSEGQPVKLLFPASLRFSGKRLL
                     VTNLSLDLRLFSPTFATVDSEWCAQVTRYTVVSIEFDTAEGAE"
     gene            complement(2174..3277)
                     /locus_tag="VGR03_11125"
     CDS             complement(2174..3277)
                     /locus_tag="VGR03_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994876.1"
                     /translation="MSNRNYWLDLFTGVTWNEFLAAGGDVSGFRESRWPAVQKIKPGD
                     YLLCYLTGVSRFIGVLESASAPFKDSSPIWKDEDFPCRLKVKTVVVLTPETAVPVVEL
                     RDRLSMFREMKSAIAWTGHFRGSPAKWKTSDGDAVLEALLEAEKNPTTRPVDAAKLAR
                     RPKALKAKMGSVTVPESEEVSTEKPEEIREPSDHQEIQWLLLKLGSDMGFDVWVARND
                     RGRQLRGQRFAELPKLKTELPLQFDEATNRTIELIDVLWLKGNAIVAAFEIESTTSIY
                     SGLLRMSDLIAMQPNLNIPLYLVAPDERRQKVIDEVNRPTFRHLSPPLFEVCRFISFT
                     DLRTRLKEAGPLIRYLKPEFLEELSESCEIEEA"
     gene            complement(3489..3704)
                     /locus_tag="VGR03_11130"
     CDS             complement(3489..3704)
                     /locus_tag="VGR03_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994877.1"
                     /translation="MKFFIPFALSFIALVVLFAPGIAPVLADATVYVLLVVVDYAVMR
                     MVVGSAQGDSLARGRSGARKNGPSEKG"
     gene            complement(3803..4666)
                     /locus_tag="VGR03_11135"
     CDS             complement(3803..4666)
                     /locus_tag="VGR03_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994878.1"
                     /translation="MPPTEGKSRKTGKMRETPDCATISTEKAEMSKRAPAYALEPMER
                     FTEYSESANTLLYLSMRAISMTRGVPKMFEVIASVGREAAGWDEQAHKTALEKMKNDA
                     DFAAKECDTHFPLLHEFTLVGMWGAFEAAIEDLVVAILCNEPELLRADPFSKIRIPLA
                     DFETLEREDRMRILLRELQRSFRSDQRQGVKAFESILGTVALSGEVKPADREGIWEMN
                     HARNVIVHRGSCADRTFVAACPKLGLKIGDRIQITDHLLGRYVGSLGRYAVEIIYRIG
                     TRYGVDMGARP"
     gene            complement(5733..6707)
                     /locus_tag="VGR03_11140"
     CDS             complement(5733..6707)
                     /locus_tag="VGR03_11140"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEV1994879.1"
                     /translation="MAKKSSRQISPTPADPARLRVGPAGWSYPDWAGYVYPARKQKGF
                     HEAAYLANFFDVIEINTSFYQPIRPEHARLWIEKVAANPRFVFTAKLWQKFTHDIISA
                     SGGASAEDERAVRAGFDVLRQAGKLGAVLLQFPFSFHRTTETAVYLGAVLKRFSDYPL
                     VVEVRHASWQAEETFELLRKHGAGFCNIDQPVIGRSMKPSAEATSPVGYIRLHGRRYD
                     TWFRDEPSTPPHERYNYLYSAEELAPWVARTRKVLEQSRDVFVVANNHYQGKAVVNAL
                     QLISLLKGEKVKVPEPLRQHYPELDAIAAEPPAEPTLFPLGSSPVRKE"
     gene            6922..7269
                     /locus_tag="VGR03_11145"
     CDS             6922..7269
                     /locus_tag="VGR03_11145"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEV1994880.1"
                     /translation="MNISISYRRVESQKSLEDEIQRHAGKLQKLLKNYAPDAVQLRGV
                     FSKNPRREEHSFSVNLSLPTGTLHATATDTKLRRSCKDAFAELESQVKKHQSLLRRDY
                     IWKRKRRAAAEIL"
     gene            7457..7531
                     /locus_tag="VGR03_11150"
     tRNA            7457..7531
                     /locus_tag="VGR03_11150"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7489..7491,aa:Lys,seq:ttt)
     gene            complement(7698..8483)
                     /locus_tag="VGR03_11155"
     CDS             complement(7698..8483)
                     /locus_tag="VGR03_11155"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050825 - ice binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HEV1994881.1"
                     /translation="MKKLLMLIFMLSMGSLCWAQTAPSLGSAATFAVLGSSNVTCTAP
                     GIISGDVGVSPGLFTNTTGCIVVGTVHQGDSAAINALMALNTAYQFALSHNSICTSNL
                     ADAPLLPLKLTPGVYCNNSAAGLTFTGGTLTLDASGNPNAVWIFKIAHGLTGSAFKVV
                     MINGGQPCNVFWLVGADSTSTNSTLQGNILAGGLTGSFTSTDSTLIGRVLANGAVTLT
                     RPNVHGSCAIAAQGAAGCDADDNDEHHHDKHRDDEEKDRKERD"
     gene            complement(9264..9536)
                     /locus_tag="VGR03_11160"
     CDS             complement(9264..9536)
                     /locus_tag="VGR03_11160"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1994882.1"
                     /translation="MFHLIWYILIGLISGVIAKSVMHVHMTIFWTIVLGIIGSILGGA
                     VTHMFSRPTNERYHPAGLIFSTLGAILVLFICYKLNIHFPQVLGFR"
     gene            9634..9789
                     /locus_tag="VGR03_11165"
     CDS             9634..9789
                     /locus_tag="VGR03_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994883.1"
                     /translation="METQELAVKIHTNVHPFSYSETARCAVELLVEKKQLEAKVPISR
                     VTGQVPD"
     gene            complement(10356..11444)
                     /locus_tag="VGR03_11170"
     CDS             complement(10356..11444)
                     /locus_tag="VGR03_11170"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HEV1994884.1"
                     /translation="MKKLFVLICMLSMGSLAWAQTAPSLGSAASFSALAGGPAAGAVT
                     CTTSTITGDVGVVAPGTFFNTGCTISGAINPNATGPYADFLNAYGLLPGANPVCTQTL
                     TTLDGQVLSPGVYCVAAAATSTGSLLTLNGPSNGIWIFRIGTSGTGALTGTSFSVVMA
                     GGGVPCNVYWWVAQAATMTDSNFVGTILAGADITVTRGTFIGRALAGGKGTTAIPSGA
                     VTLTDTILGGCGSSPAPATGTIKVTGGGQIQVPDPSSLGTASFGFNAGTGKDGSGGHF
                     NYVNHVTGLHVDGPVNQIVVIAFNSDGSPKTVLFSGTCGDSCAFSVIVQDNGEPGIND
                     QFGVTVTGAVTEFRSLRVISNGNIQFHK"
     gene            11737..12537
                     /locus_tag="VGR03_11175"
     CDS             11737..12537
                     /locus_tag="VGR03_11175"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994885.1"
                     /translation="MITFVSIIALGFFLGMRHATDPDHVIAVTTIVSRQRSIRHAALI
                     GVLWGVGHTITILLVGSAIILFGLVIPPRIGLTMEFSVGLMLILLGIFNLSGILRWVT
                     ETFTPSEQALHAHPHAHGDYVHSHAHGHDPENHGHREDATPVSRLDRVFGQLGLFQIL
                     RPLAVGIVHGLAGSAAVALLVLTTIRIPFWAVVYLLVFGVGTIAGMMLITAAIALPFT
                     YSQRRFVVLNRGLGLASGLISLGFGIFIVYQMGFVNGIFTSHPNWVPR"
     gene            complement(12569..13726)
                     /locus_tag="VGR03_11180"
     CDS             complement(12569..13726)
                     /locus_tag="VGR03_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1994886.1"
                     /translation="MFADRTNWNLAPNRLSEVLARHRAAGKPLLDLTASNPAQCGFRF
                     DRETILRALSGPCALDYEPDPKGLEAARRAVAEYYARRGDRVSPGDILLTTSTSEAYS
                     FVFRTLCNPGDEMLVPEPSYPLFAFLADIQDVRLVRYPLLYDAVWRIDFPALERAITP
                     RSRGVIVVHPNNPTGHFCKAEEMARLNQLCASRGLAIVADEVFLDFSLGGHPPASFAS
                     NAAALTFTLSGLSKIAGLPQMKAAWLVTSGPEPWKTQALARLELIADTYLSMNAPVQH
                     AIPVFLEQRHAFQQQLIARVRRNLAELDHRLAEQKSCTRLPVEGGWYAVLRVPATRSD
                     EDLAIELLASQSVYVHPGHFYDFPADGYFVVSLITPENDFAEGTRRLLAQF"
     gene            13941..14516
                     /gene="hpt"
                     /locus_tag="VGR03_11185"
     CDS             13941..14516
                     /gene="hpt"
                     /locus_tag="VGR03_11185"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV1994887.1"
                     /translation="MKKTKIAASKPGESVRVLLTRSRIQHRIAALAREIHRDFRGEPV
                     HLIGVLKGAVFFLADLIRCLPGEVSLDFIAVSSYGKETQSSGQVMLTKDLDSSIEGRN
                     VLLVEDILDTGQTMHYLLRILDQRRPKRLRVAVLLDKPERRQVAVTADYVGFRIPNEF
                     VVGYGLDYAERYRNLHDVSVLSLGGKAKSAE"
     gene            14791..15714
                     /locus_tag="VGR03_11190"
     CDS             14791..15714
                     /locus_tag="VGR03_11190"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV1994888.1"
                     /translation="MSGSISGAIVDQTGAPVAGARVRLTREDQSANQEALSDEDGQFT
                     FANIAPGPFQLTITSAGFAAQTSSGTLRLGENYSVPQITLAIATATTEVRVALSPAEV
                     AEDQIKEQEKQRVLGLIPNFYVSYVPNAVPLTSKQKFELAWRTSVDPVTFVVTGAFAG
                     VQQAQNDYSGYGQGAQSYGRRYGASYADLVISTFIGSAMLPSLLRQDPRYFYKGSGSR
                     RSRILYAIANSVICKGDDGHWQTNYSGILGSVAAGGISNLYYPAKDRGAGRTFESAVI
                     GIGESAVVNLFQEFFVRKLTPKVLNHQPAKP"
     gene            15969..17741
                     /locus_tag="VGR03_11195"
     CDS             15969..17741
                     /locus_tag="VGR03_11195"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEV1994889.1"
                     /translation="MNPTDAPTQPVETTPEAQVSAEVLQTLVEIGEEVNASLDLDEVL
                     ARAAALIKRLIDYEFFGVLLVQQPGNYLKHRFAIGYPRELADSLRVPLGQGITGSAAA
                     AGHAVRVSDTSKDPRYINAIESVRSELAVPLLISGNCIGVLDIQSRHLDYFTHEQQNI
                     LTLVAGRLAVAIENARLFERVRAQANTLLVLSDVSRETSAILEVEELLRRAAEQIKRV
                     IDYQILSLMLYDDEQKIFRHRLDVKYGHHVQGKLRVAATEGIVGAAATLREAVLVPDV
                     TADPRYLMVNPETRSELALPMILKGRVIGVLDLESPQLDYFTEDHVQTLSILAANLAV
                     SIENARLYEKSAREEARLERELQAAKRIQGALLRPVPAEDYGLDMAARYLSAREVCGD
                     LYEFSRYGPRQLGIALGDVSGKGTAAALFGAVAIGILRSLAPQKLRPAELLRQINHLI
                     GERRIEGRFMTMCFATWEKGRQKLRVANAGQSQPLLYRGGRCDKINLTGLPLGIFEEA
                     NYDEWESTLDSGDILVFHSDGIAETANPDGQFYGTGRLRKLVEEHAQRAAAEIAELIL
                     RDVDGFAQGLPLADDRTLVILKVR"
     gene            17749..>18141
                     /locus_tag="VGR03_11200"
     CDS             17749..>18141
                     /locus_tag="VGR03_11200"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994890.1"
                     /translation="MAIRASKGSPRKPRYVDPASFTPQFSWKQTKQGEAVFAEGVALP
                     DVAEKAGTPTYVYSRSAIERAYRELDSGLAGVAHTVCYAVKANSNLSLLRILARLGGG
                     FDAVSGGEIERLRRAGIAGNRVVFSGVGK"
BASE COUNT         3782 a         5368 c         5251 g         3740 t
ORIGIN      
        1 gaagcgtccg cccttcttgc ctgctcaatc cttgcaccgc tcgccgccct ccttttctca
       61 tcactgtcaa cattgaactt tcaactttta actttcaact ttgaactttg aactttgaac
      121 tttcaacttt caactctcac cttccccctc cccctcccac gggttcaacc agaaatccag
      181 cgacagccac aggctgtacg cataaggcat gaaaacgagc gacagagcgg cgccgaacgc
      241 aatccagaga gcgagtatcc tgttcacgga aagctgcgta tccggcagca acagggacac
      301 gagatatacg gcgatcagca cgattgcggt caggccgtac gtaatcatca ttccgccgat
      361 gtaatagccg gactcgcgga aaaaagaaag cccgcagacc gggcagcgcg gcaacatctt
      421 gttgggccat cgcgcgagca cacgccctcg ccggcagttc gggcatcgct tccgcagcgc
      481 gtcacctaca gcttttccca acgccggttt catgggtggc ttccatgcct cttgcatctt
      541 aatcgaaata ccttcccacg ccgcctccgg gcttctctgt gccctccgtg acctctgtgt
      601 taaatcctct ctctccacgg tttttctgtc atcccgcctg ctccggactg ctcggggacg
      661 ccgctttgct cttgtcacta cgcaaccgta agctcagcca gtcgcccttc ccgcgtgaac
      721 ggcgctgagg catttttgtt gagatgcagg acgtagaatg ggcgtagaat accggcccaa
      781 ggtaggtagt ctcacttcct ctggagcgcc ccctccttgc tcgtcgggcg cgcagactac
      841 cttgccaaat tcaagcggag gttctaacca tgcggcgcgc gagccccttg atagcgtttg
      901 cccttgtagt tctcctcgga ttgcccctcc aggcagtgcc cttcaggagg ggccatgtga
      961 aggtctttgc ggttcttcca gacgggtcgt caggccccga ggggcttgag gtcgactccg
     1021 ccggcaatgt ctacgtgacc acgttcggct tcaccgcgaa gggttccgcg tcggggccag
     1081 ggcagctctt cgtgttcaac gcgcacggcg acctcttgcg gcaactctcc attgctgggt
     1141 catccccgca cctgctcggt ctgcacttcc atccgctgac cggagcgctc ctggtgtgcg
     1201 atttcggcgc cggaaaggtt ctcgacgtgg atccgcggac cggtgctgcg gtcgtcttca
     1261 tgacgctacc ggctctgccc cacccaagcc tgggcgccgg cctcaacgac gtcacctttg
     1321 acgcggcggg aaacgtctac gtgtcggatt ctttccaagg cattgtctgg cagaccggtc
     1381 cccacggggg tgtcgggacc gtctgggtgg acgacccgct gttgcgcacg actggcgttc
     1441 cacccttcgg ggccaacggc ctccgcttca ataaggaaga gacggcgctc ttcgtcgcca
     1501 acaccggcaa cgacacaatc gtgaaggtat ctgtcgctct gaacgcagca ggccagcgca
     1561 cagcggcggt gtttacgaac agcatcggcg gtgccgatgg cgtgatcctc gacgataaga
     1621 acaacctctg ggtcgccgcg aaccagtctg acgagatcgt ggtcgtggat cccaccgggc
     1681 gggtgattga aaagctcggg gacttcgccg gcgtcaactc cgaaggccag ccggttaagt
     1741 tgctctttcc cgcgagtctg cgcttcagcg ggaagcgtct gctggtcacg aacctctcgc
     1801 tcgacctgcg gctcttcagc ccgaccttcg ccaccgtaga ctcggaatgg tgtgcccagg
     1861 ttacgcgcta cacggttgtt agcattgagt tcgacacggc agagggagcc gaatgaccgt
     1921 tctctgtcta ttcgaaccgt gccgttcagc ccgtcacgcc accaccccgc ctttggcctt
     1981 cccacaagcg atgcaataac tacctaagcg gaggcctggc ggctttgcca agagtccaag
     2041 ggactctcgg aagtactcga cccccttttc aattggctct tcacatgtgt cacaggttct
     2101 tcgaactcgc gcctttgtca gtgtgagtat tttggccgct tcatatttgt tcatgacagc
     2161 atcgctcgcc gctctaagcc tcttcgattt cgcaagattc tgaaagttct tctagaaact
     2221 caggcttcaa gtagcggatt agcggaccgg cttctttcag ccgagtgcgc aggtctgtga
     2281 aagaaatgaa tcggcacact tcgaagagcg gtggcgagag gtgtctgaag gtagggcgat
     2341 tgacctcgtc gattaccttc tgtctccgct catcaggcgc gacgagataa agagggatat
     2401 taagatttgg ttgcatggct atcagatcgg acatgcggag aagacctgaa taaattgagg
     2461 tcgtgctctc gatttcgaat gctgcaacaa tggcgttgcc cttcagccac aacacgtcaa
     2521 tcagttcgat agttcgattt gtagcttcgt cgaattgtag tggcagttct gtctttaatt
     2581 tcggcaattc ggcaaaccgc tgacctctta actggcgtcc tcggtcgttg cgggcgaccc
     2641 acacatcaaa tcccatatca ctgcctagct tgagtaagag ccattggatt tcttgatggt
     2701 cgcttggctc ccttatttcc tccggctttt ccgtagaaac ttcttcgctc tcaggaacag
     2761 ttaccgaccc cattttcgct ttgagtgcct taggtcgcct agctagtttt gccgcatcca
     2821 cgggccgtgt agttgggttc ttctccgcct ccagcaaggc ttctagcacc gcgtcaccat
     2881 ctgaggtctt ccattttgca ggggaacctc ggaaatgacc cgtccacgcg attgcgctct
     2941 tcatttcacg aaacatactt aagcggtccc gcaactccac cacgggaaca gctgtttctg
     3001 gcgtcagcac gacaactgtc ttcaccttaa ggcggcaggg gaaatcttca tccttccaga
     3061 tcgggcttga gtccttgaaa ggggcggagg ctgattcaag taccccaata aagcgagaaa
     3121 caccggtaag gtagcaaagc aaataatcac cgggcttaat cttttgtacg gcaggccatc
     3181 gactttctcg aaaaccggac acatctccgc ccgccgccag gaattcgttc caggtaacgc
     3241 cagtaaaaag gtccagccaa taattgcggt tactcatgac tacgactcga aatgctggtc
     3301 gctccgtgcg ccggattata gcagggagca atactcacta ctcccacaac ctcggtcttg
     3361 ccgtccctcc gggttcgccc aattcctatc ccctttcccg ccagccctcc ctccatcgct
     3421 tgtccgcaat ctctgtgccc tctggcgggt ggcaaaccct tcccgctcaa agtgagcctt
     3481 gggctacctt accctttttc ggaagggccg ttctttctcg caccggagcg cccccgggcc
     3541 aatgagtccc cctgcgcgga gccgacgacc atgcgcatca ctgcgtagtc cacaaccacc
     3601 agcaagacgt agacggtggc gtcggcgagg acgggcgcga tccccggcgc aaaaagtacg
     3661 acgagggcga tgaagctgag tgcgaaaggg ataaagaatt tcattggtca gaaaagtgat
     3721 tggggttgat ttgtttttgg agtgaacgct ggcggaaggc ttatccgagc gcgggcgcaa
     3781 gtgtggacaa tgcagagcca ctctaaggcc tcgctcccat gtctacgccg taccgagttc
     3841 ctatgcgata aatgatttct actgcatatc gaccaagcga accgacgtag cgtccgagca
     3901 ggtgatcagt tatctgaatt cggtcgccaa ttttaagtcc tagcttcggg catgcggcga
     3961 caaaagttct gtcggcgcag gaaccccgat gcacaattac gttgcgggca tgattcattt
     4021 cccagatacc ctctctgtcc gcgggtttca cttcgcccga tagggcaacg gtacccagga
     4081 tcgactcgaa agctttgact ccctgccttt ggtccgagcg gaacgatcgc tgcaactctc
     4141 tcagtaaaat tctcatccgg tcttctcttt cgagagtttc aaaatctgcc aaggggatac
     4201 ggattttgga aaacggatcg gcgcgcaaaa gctcaggctc gttgcagaga atggcgacta
     4261 ccaagtcctc gattgccgct tcaaacgcgc cccacatccc gacgagggtg aattcatgta
     4321 acagcggaaa gtgggtatcg cactctttag cggcaaagtc cgcatcgttc ttcatttttt
     4381 cgagggcggt tttatgggct tgctcatccc aaccagccgc ctccctgccc acactggcta
     4441 ttacctcgaa cattttgggg acgcctcggg tcatcgatat tgcgcgcatg ctgaggtaca
     4501 acagcgtatt cgcagactcg gagtactccg tgaacctttc catcggctct agggcgtatg
     4561 caggggctcg tttactcatt tcagccttct ccgtggaaat agtagcgcaa tctggcgttt
     4621 cccgcatttt gcccgttttt cgcgattttc cttcggtggg cggcaaaccc ttcccgctcg
     4681 aagcgacgct tgcgtgccgc gccgctcacg gggatggcct gcccgttgca gcctcggcga
     4741 aggtcgagca gacccgccgc aggtcggaag acccgccgca ggtcggaaga cccgccgcag
     4801 gttggcgaga gggcgagtgc gccgtgcctc cgggttcact caattcctat cccctttccc
     4861 gccacccctc cctccatcgc gtgtccgcaa tctctgtgcc ctctgcgtcc tctgtggtga
     4921 acctcccctc accctcccgc ctctatcccc tttcccgcca gccacttaca attattcctt
     4981 gacaagtatc taactcttgt ggtaacatgc cccgtgagtt cggcggacct tcagtcctgc
     5041 ctccggcaag cccggaagcc tgccccggtc cggtgtttgg actccggtgg agtccactgg
     5101 actcccgaaa tcagggatcc cggtgcgttt gcgggaccgg agatgattca gcggggcgac
     5161 cgctctttga aaactgagca tccggcaaag gatgcgacct ctccagcatc ccgcgagttt
     5221 tgcgggatga atcctgagcg agtccgccgt ggcggccgag tcgaaggacc cctcttctaa
     5281 cccctgtaga atcaacactt gcaaatctgt atcaaaacaa agaactttaa ctccccttag
     5341 aatcaacact tacaaaaaac aggggggagc gggagtatct cttctgccaa cagtaccggt
     5401 acccactttc ctgccaaccc cgccccgctc ctggcggggt cacctatcac ccatccttca
     5461 cttcctctac ctcacctatc tcctttacct cctgcgcctc attgcttctt cggcgcaagc
     5521 gggaacagtg tcggctcggc gggaggctcc gtccaggcga ccgtagattc tgttccgtgc
     5581 ggaatcgtag gttccgctcc tggcggaatg gcggattccg gctcggtcgg aaagaatctg
     5641 cgctttctgc atcccgtatg aatctgcggg acgcgcttgg cggaaaggct ttttcttcca
     5701 tccctcacgg accacagatc acgtttcccg gctcactctt tgcggacggg ggaactgccc
     5761 agcgggaaca gcgtcggctc ggcgggaggc tccgccgcta tggcgtccag ctcgggataa
     5821 tgctgccgca gcggttccgg caccttcacc ttttctcctt tcagcaggct gatcaattgc
     5881 agagcgttca ccacggcctt cccctgatag tggttgttgg ccacgacgaa aacatcgcgg
     5941 ctctgttcga gcactttccg ggtgcgcgca acccacggcg caagctcttc ggccgagtag
     6001 agatagttgt aacgctcatg cggcggagta ctcggctcat cgcggaacca ggtgtcgtac
     6061 cggcggccgt gcagccggat atagccgacc ggcgaagtcg cctccgccga cggcttcatc
     6121 gagcggccga tcaccggctg gtcaatgttg cagaagccgg cgccgtgttt ccgcagaagc
     6181 tcgaaggttt cctctgcctg ccacgaggcg tggcgcacct ccaccaccag cgggtagtcc
     6241 gaaaagcgct tcagcaccgc gccgagatag accgccgtct ccgtagtgcg atggaaggaa
     6301 aacggaaatt gcagcagcac ggcgccgagt tttccggcct gccgcaacac gtcgaatccg
     6361 gcgcgcacgg cgcgctcgtc ttcggcgctg gcacccccag aagcggagat tatgtcgtgc
     6421 gtgaatttct gccacagctt cgccgtgaac acgaagcgcg gattggccgc gaccttttcg
     6481 atccacaggc gggcatgttc cgggcggatg ggctggtaga aagaggtgtt gatttcaatg
     6541 acgtcgaaaa agttcgccag gtaagccgct tcgtgaaagc ctttttgctt cctcgcggga
     6601 tagacgtaac cggcccagtc gggatacgac cagcccgccg ggcccactcg cagccgcgcg
     6661 ggatccgccg gagtgggaga aatttgacgg ctggattttt tggccaagtc attttcgcgg
     6721 caggaacttc cgcgccgcgt ccgcaagcga gcctagggaa tgccctccgc cgtgtcaacc
     6781 cactccggac gcggattccg cggcaacgcg gcaggcggat tgcctcgcgt cgtgcagcct
     6841 gttatcctga aaacgtcatg cggtgcagaa cgctgaactg cgtcgccttc ctcccggtgc
     6901 gatcccccta caggtgcaaa catgaacatt tccatcagct acagacgcgt agagtcgcag
     6961 aagtcgctcg aggacgaaat ccagcggcac gcggggaaac tgcaaaaact gctgaaaaac
     7021 tacgcgccgg atgcggtcca gcttcgtggc gtattctcga aaaacccgcg ccgggaagag
     7081 cactccttct cggtgaacct ctcacttccc accgggacgc tgcatgccac ggcgacggac
     7141 accaaactcc gccgaagctg caaagacgct ttcgccgagc tggaatcgca ggtgaagaag
     7201 caccagtctc tgctgcggcg ggactatatc tggaagcgca agcgccgcgc cgcggctgag
     7261 attttgtagt ccgtgccccg agccgcgaag ctgcgaggcg cgaagctgcg aggcgcgaag
     7321 ttgcgaggcg caaacctgcg aggcgtaaag ttgcgaggcg cgaagttgcg aggcgcaaaa
     7381 ccgcgaggcg caaagatgcg atgctcgaag ttgcggcccc tgctcgtaaa cgctaagatg
     7441 aaaagatgct gccagcaggg gcgtagctca gtggtagagc agcggccttt taagccgaag
     7501 gtcgtgggtt caatccccac cgcccctacc agttccaagc gaatcctggt caaagtgtaa
     7561 gcgaagtttt caccaataag acgaagaccc accctttcgc aacgtcgcga aaggatgggc
     7621 cacctgcccg tctcgcctag ccctgagtgc ccggaggcgc ggcatgagcc gcgcctccga
     7681 acactcctca ggttaggtta gtcgcgttcc ttacggtcct tctcctcatc gtcgcggtgc
     7741 ttgtcgtgat gatgttcgtc attatcatcc gcgtcgcagc ctgctgcgcc ctgagccgcg
     7801 atggcgcagg atccgtggac gttggggcga gtcagggtca cggcgccatt cgccaagaca
     7861 cgtccgatta aggttgagtc ggtactagtg aagctcccgg tcagcccacc tgcgagaatg
     7921 tttccctgga gcgtcgaatt ggtagatgtg ctatcggcgc cgacgagcca aaacacgtta
     7981 cagggttgcc cgccgttgat catgaccacc ttgaacgcgc tgccggtgag gccgtgggcg
     8041 attttgaaaa tccagaccgc attgggattg ccactggcgt cgagtgtcaa ggtgccgccg
     8101 gtgaacgtta gccccgcagc cgagttgttg caatacacac ccggcgtgag cttgagtggc
     8161 aggagcgggg catcggccag gttactggtg caaatgctgt tatggctaag cgcaaactga
     8221 tacgcggtgt taagggccat cagagcgtta atggcggccg agtcgccctg gtgaactgtt
     8281 cccacaacaa tgcagccggt agtatttgtg aaaaggccgg gggagacgcc gacgtctccg
     8341 ctgattatgc cgggggcagt gcaggttaca ttcgagctac ccaggactgc gaaggttgcc
     8401 gccgagccca gtgatggcgc cgtctgcgcc cagcacaggc tccccatact caacatgaaa
     8461 ataagcatca aaagtttttt catttcaaat ctccctggtt ggattcctct tcatcgtcgc
     8521 tgacagtgcg ggacaaacat tgtcgagtca accttgagat accaagccga agcggatgtt
     8581 aacagccagt gagatcggca tctgtacact attgaacacc gtcgaaacaa actgggcaga
     8641 ggcggtctgg cagtactacc agtacgttaa ttcattttta agcccgcaat ccgcaagccg
     8701 cgatagattc aggtgcttat gatggtcgcc tacgcaatga aatattgatg cggcagcgaa
     8761 gtggctgcac gcaaacaatc tgggaagtag tcctaaacaa gaaccatatc gacaagtggc
     8821 tggcaaaggg cattccagat acgcgaaata aagcagcggc cttttaagcc gaaggtcgtg
     8881 ggttcaatcc ccaccgcccc taccaacgta tcccattgaa aagtatgagc aactcatttc
     8941 gttacaatcg tgtgcagacc gttacacctt tgtttcggtg ttggtcttcg tttgaatgcg
     9001 tctcctctct tttgcctgct ttgagtggag aacaacccgg agtttgactg tgggagcgag
     9061 gcgattccac ttctccggta agcgcgttaa tcaggcatgt gaattacttc cgagtatccc
     9121 gtttgccagg aactgggtgc gttcgcgtta ggtctctcgg ggaggagtga tttacctaaa
     9181 cccgagtgtg cccacagatt tccctcttac gagggatttt ttgcaaggtg gcagtgttcc
     9241 aagcagcccg agacgtgacc gtcctatcgg aatccgagga cctggggaaa atgaatgttc
     9301 agcttatagc agatgaagag aaccaggatc gcgcccagtg tggaaaaaat gaggccagcg
     9361 ggatgatatc gttcgtttgt cgggcgcgaa aacatgtggg taacagcgcc gccgaggatc
     9421 gatccgatga tgccgagcac gatcgtccag aagatcgtca tgtggacgtg catcaccgat
     9481 ttcgcgataa cccccgagat tagcccaata agaatatacc agattaagtg aaacatactt
     9541 tttcctccac catttgcctg cagatcgtcg tcgagtataa cccagggttt aacaatgaac
     9601 caaactttcg tatggtattg atcggctttc caaatggaaa ctcaagaatt ggccgtcaag
     9661 attcatacga acgtacatcc cttcagctac agcgagaccg caagatgtgc agtcgaattg
     9721 ctcgtcgaaa agaagcaact ggaggccaaa gtcccgataa gtcgggttac cggacaagtg
     9781 ccggactagg actccactgg ttgcagaagc aggaaatgtt acctgtcagg gacaaaaaca
     9841 aagggcgctt gttaagagtc acaagcgccc ttgaattaaa tcaattcgtt gaatacctca
     9901 gggatcacct ccttctcgac aagtgcgtcg attgaagcct gaaactattc ttatcacggt
     9961 gaatccctat gtgtctacaa caccggcgat gtttgaggag atgcgctttg cagtgcctgg
    10021 taagtcggat tatcggaagg tgactcgctg cacatgcatc gggcgacaag gggtagaaca
    10081 actggaatag aaggcccgga gcggtccaaa gtcttgagaa gaaatcggta agtcaacgcg
    10141 cgaaaggtac tcttaagccg acaggtcgtg ggttcaatcc ccaccgcccc taccagttcc
    10201 aagcgaatcc gggtcaaagt gtaagcgaac ttttcatccg taagacgaag acccatcctt
    10261 tcgcaacatc gcgaaaggat gggccatctg ccatgaatac acggaggcgc ggcatgagcc
    10321 gcgcctccag ggacccagtc gtctcaggtc aaccgctact tatggaactg gatattcccg
    10381 ttgctgatta cacgcagcga tctgaactcg gtcactgcac cggtcaccgt gacaccaaac
    10441 tgatcgttga tgccaggctc gccgttgtct tgcacaatca cgctgaaggc gcaactgtcg
    10501 ccgcaggtgc cggagaacaa gaccgtcttc ggagagccgt ctgagttgaa tgcgatcacc
    10561 acgatttggt tgaccgggcc atcgacgtgc aggccggtta cgtggttgac atagttgaag
    10621 tggccgccgc tgccgtcttt gcccgttcca gcgttgaagc cgaagctcgc agtgccgagg
    10681 gagctgggat caggcacttg gatttggccg ccgccggtta ccttgatggt tccagtcgcg
    10741 ggcgcggggc tgctgccgca accgccgagg atggtgtcag tcaacgtcac tgcgcctgac
    10801 ggtatcgcgg tcgtgccctt cccgcccgcc aaggctcgcc cgatgaacgt tcctcgcgta
    10861 acggtgatgt ccgcacccgc gagaatcgtt cccacgaagt tcgaatccgt cattgtcgcg
    10921 gcttgggcga cccaccaata cacgttacac ggcaccccgc cgccggccat gaccaccgag
    10981 aagcttgtgc ccgtgagcgc gccggtcccg ctggtcccga ttctgaagat ccagatcccg
    11041 ttcgagggcc cgttgagtgt caacaagctg cccgtcgacg tcgccgccgc ggcgacacag
    11101 tagacaccgg gcgaaagcac ctggccatcg agggtcgtca aggtctgggt gcacacgggg
    11161 ttggcgccgg ggagcaaacc gtacgcgttg aggaagtcag cgtaaggccc cgtggcgttc
    11221 gggttgatcg cccccgaaat cgtgcaaccg gtgttaaaga aggtgccggg cgcgacgacg
    11281 cccacgtctc cggtgatagt ggaagtggta caagtgaccg caccggccgc cggaccgcca
    11341 gccagggccg agaagcttgc cgctgagccc agtgatggtg ccgtctgcgc ccaggccagg
    11401 ctccccatac tcaacatgca aataagcaca aaaagttttt tcatttcaaa tctccctggc
    11461 tggattcttc ttcatagtcg ctgacagtgc gggacaaaca ttgccgagtc aaccttgaga
    11521 taccaagccg aagcgaatgc taacaggcag cgcaaagcgg catctgtacg ctattgaaca
    11581 ccgtcgaaac aaacagggcg gaggtggtct ggcagtacta ccagtacgtt agtttattgg
    11641 tttttccagt ttcgaacgct gctttcctgt gttcgctcgg ctgaggatgg cacgtgctcc
    11701 ggcgcgtggt aggattcacc tcgcgggtag gggggaatga tcaccttcgt ctccatcatc
    11761 gcactgggat tcttcctggg gatgcgccac gcgaccgacc cggaccatgt catcgctgtt
    11821 accaccattg tcagccgcca gcggagcata cggcatgcgg cgttgatcgg cgtcctgtgg
    11881 ggagtaggtc acaccatcac gattcttctc gtgggatccg ccatcattct attcggcctg
    11941 gtgattccac cgagaatcgg ccttacgatg gagttttccg ttggcctgat gctgatcctg
    12001 ttgggaatct tcaacctgtc gggaatcttg cgctgggtta cggagacgtt tacgccttcc
    12061 gagcaagcgc tgcatgcgca tccgcacgct cacggagact acgttcacag ccacgcgcac
    12121 gggcatgacc cggagaatca cggtcacagg gaagatgcga cacccgtgag ccggctggat
    12181 cgggtatttg ggcagttggg actttttcag attttgcggc ctttggctgt gggtatcgtt
    12241 cacggcctcg ccggatcggc ggcggtcgcc ttgctcgtcc tgacgacgat tcgcattccg
    12301 ttctgggcag tcgtttatct gcttgttttt ggcgtcggca caatcgccgg catgatgctg
    12361 atcaccgcag cgattgcctt gccattcaca tactcccagc gtcgcttcgt cgtgcttaac
    12421 cgcggcctgg ggctcgcgtc cgggctgatt agtctgggct tcggcatctt tatcgtttat
    12481 caaatggggt tcgtgaacgg gatctttacc agccatccca attgggtacc gcgttgaggt
    12541 atcggacgcg ctcgaccgat cacaacgatc aaaattgcgc gagcaggcgc cgcgttccct
    12601 ccgcgaaatc gttttcgggg gtgatgaggc tcacgacgaa atagccgtcg gcggggaagt
    12661 cgtagaagtg gcccggatgg acatagacgc tctgactcgc cagtaactct atggcgaggt
    12721 cctcatccga acgcgtcgcg gggacgcgga ggacggcgta ccacccgcct tcgacgggca
    12781 agcgggtgca ggatttctgt tcggccaggc ggtgatccag ctcggcaagg tttcgccgca
    12841 cgcgggcaat caactgctgc tgaaatgcgt gccgctgctc caagaaaaca ggaatcgcat
    12901 gttgcaccgg agcgttcatc gaaagatagg tgtcggcgat gagctcgagg cgagcaaggg
    12961 cctgcgtctt ccagggctcc ggcccgctgg tgaccagcca ggcggccttc atctgcggaa
    13021 ggccggcgat tttcgaaaga ccgctcaggg taaaggtgag cgctgcggcg ttcgaagcaa
    13081 agctggccgg cggatggccg ccgagtgaga aatccaggaa gacttcgtcc gcgacgatgg
    13141 cgaggccgcg ggaggcgcag agctgattca agcgcgccat ctcttccgct ttgcagaaat
    13201 gccccgtggg attattgggg tgaacgacga tgacgccgcg cgagcggggc gttatggcgc
    13261 gctcgagggc ggggaaatcg atgcgccaga cggcatcgta gagaagtggg taacgcacca
    13321 gccggacgtc ttgaatgtcc gcgagaaaag cgaagagggg ataactaggc tcggggacga
    13381 gcatttcgtc gccgggattg cagagcgtgc gaaaaacaaa cgagtaggct tcgctggtgc
    13441 tggtggtaag aagaatgtcg ccgggggaga cccgatcccc tcggcgggcg taatactccg
    13501 caaccgcgcg ccgcgccgcc tccaggccct tcggatccgg ttcatagtcg agagcgcacg
    13561 ggccggagag ggctcgcagg atggtttccc ggtcaaagcg aaacccgcac tgcgccgggt
    13621 tcgaagcagt caggtccagc aacggctttc ccgcagcacg atgccgcgcc agaacttcac
    13681 tcagccggtt cggggcgaga ttccagttcg tgcggtcagc gaacatttgg tgcgcgccct
    13741 gctgaaatcc cgctgccgtc ggccatgatg gtgaagcact gcgcggccaa cccgacggcg
    13801 ttcggaaaga tttgcgacat agaaacgcga agggtagcac gaaatgccgc cgcatccgtt
    13861 gcgcgccgtt accttgccgg gagaacggga catgggttag agttgccgcc gggtgcgcga
    13921 gaagcggacg gaatggagcg atgaaaaaga caaagattgc ggcgagcaag cccggcgaga
    13981 gcgtccgggt attgctcacg cgcagccgca tccagcatcg gatcgcggcg ctggcgcggg
    14041 agattcaccg cgattttcgc ggcgagcccg tgcacctgat cggcgtgctg aagggcgccg
    14101 tgtttttcct ggcggacctc atccggtgcc ttcccggcga agtgtcgctc gactttatcg
    14161 ccgtatcgag ctacgggaag gagacgcaat cgtcaggaca ggtgatgctg acgaaagacc
    14221 tggactccag catcgaagga agaaacgtgc tcctcgtcga agacatcctg gacacggggc
    14281 agacaatgca ctatctcctg cgcatcctcg accagcggcg gccgaagcgc ctgcgggtcg
    14341 ccgtcctgct cgacaagccg gaacgccggc aggttgccgt caccgcggac tacgtcggtt
    14401 tccgcattcc gaacgaattt gtcgtgggtt acggcctgga ctacgccgaa cgatatcgga
    14461 atctgcacga cgtctccgtc ctgagtctcg gcggtaaggc caagtccgcc gagtaacgca
    14521 gcaccggcga cgcggcgacc gataactcat taaacgccga ccttggcgga aatccaaaat
    14581 tccttgacaa agcatcataa tgaacagagc gcaatgatta tgcctagata cacggcttgg
    14641 gtattccggc taagcctaag tgtcctgctt tggggctttc cagctcaggc ccagtctcag
    14701 caaactccta tcgtcccggg ggtggagaat ggtctgcctg acgcagcggg aacaggggca
    14761 tcagccagca ggcaggagcc cgatcagcga ttgtcaggaa gcatcagcgg ggcaattgtt
    14821 gatcaaactg gagcccccgt cgccggagcg cgggtgagac ttacacgtga agatcaatcc
    14881 gcaaaccaag aggcgctatc agacgaggac gggcaattca cctttgccaa catcgctccc
    14941 gggcccttcc agctgacaat aacgtcggcg ggtttcgcgg cgcaaacgtc ctctggaacc
    15001 ctacgtttgg gggagaacta cagtgtcccg cagatcacat tggccattgc taccgctacc
    15061 actgaggtgc gggtcgcact gtcaccggcc gaagtggcgg aagaccagat caaagagcaa
    15121 gagaagcagc gcgtgctcgg cctcattcca aatttctatg tcagctacgt ccccaatgca
    15181 gttccgctca cgtcgaagca aaagttcgaa cttgcctgga ggacgagcgt ggatccagtc
    15241 accttcgtgg tgactggggc ctttgcggga gttcagcagg cgcaaaatga ttacagtgga
    15301 tatgggcaag gggcgcaaag ttatggaagg cgttacgggg cctcctacgc cgaccttgtt
    15361 ataagcacgt tcatcggaag cgccatgctg ccatcgcttc tgagacaaga tccacgttat
    15421 ttctacaagg gaagcggcag caggcgatca cgaatactgt atgcgattgc caattccgtg
    15481 atttgcaagg gcgatgatgg acattggcag acaaattact cgggtatcct cgggagcgtc
    15541 gccgcaggcg gtatctcaaa tctctactat ccagcgaaag atcgcggagc agggcgaacg
    15601 ttcgaaagcg ccgttattgg gattggcgaa tctgccgttg tcaatctctt tcaagaattt
    15661 ttcgtccgga agctgacacc gaaagttctt aaccaccaac cggccaaacc ctaaagcagt
    15721 gtcccggtct tttgcggcgg gcggcaccgc aatctgcacg atgtctccgt ccccagcctc
    15781 ggcggcaagg ccaagccggc tgaatgatcc aggctattgg tccaatccca accgcccgga
    15841 cgaacgcacc attgggctct cagggaacgg ccaattcggc aagcaactgc cacagccttt
    15901 cgaaacatcg cgaatggatt gcgctcacaa agggcgttcg catcaccctg ctataatgaa
    15961 gggctgcgat gaatcccacc gacgcaccta cacagcctgt tgaaacaacc ccggaggcgc
    16021 aggtctccgc ggaggttttg caaacgcttg tcgaaatcgg ggaagaagta aacgcttccc
    16081 tggacctgga tgaggtgctg gcgcgggcgg cggcgctgat caagcgtttg attgattacg
    16141 agttcttcgg agtgttactg gtccagcagc cgggcaacta cctgaagcac cgttttgcga
    16201 tcggctatcc gcgcgaactc gcggacagcc ttcgggttcc cctcggacag gggattacag
    16261 ggagcgcggc ggcggcaggg catgccgtcc gtgtcagcga cacaagcaaa gacccgcggt
    16321 acatcaatgc gattgaaagc gtccggtccg aattggccgt cccgctgctc atcagcggga
    16381 attgcatcgg cgtcctggat attcagagcc gccatctcga ttacttcacg catgagcagc
    16441 agaacatcct cacgctcgtg gccgggcgat tggccgtggc catcgaaaac gcgcggctat
    16501 tcgagcgggt gcgcgcgcaa gccaacacgc tcctcgtgct gagcgacgtc agccgggaaa
    16561 cgagcgcaat cctggaggtt gaggagctgc tgcgccgggc cgccgaacag atcaagcgcg
    16621 tcattgacta ccagatcctc agcctgatgc tgtatgacga tgaacagaaa atcttccgcc
    16681 atcgcctcga cgtaaagtac ggccaccacg tgcagggcaa actgcgcgtg gcggcgaccg
    16741 agggcatcgt cggtgcggcg gccacactgc gcgaagcggt cctcgtgccg gatgtgacgg
    16801 ccgacccgcg ctacctgatg gtgaatccgg agacgcgctc cgaactcgcg ctccccatga
    16861 ttctcaaggg ccgtgtgatc ggcgtgctgg acctcgaaag cccgcagctc gattacttca
    16921 ccgaagatca tgtgcagacg ctctccatcc tcgcggcaaa cctcgctgtt tcgatcgaga
    16981 acgcccgcct gtacgaaaaa tcggcgcggg aggaggcgcg gctcgagcgg gagctgcagg
    17041 cggcgaagcg cattcaaggc gctctgctgc gccccgttcc ggcggaagac tacggcctgg
    17101 acatggccgc ccgctatctc tccgcgcgcg aggtctgcgg ggatctttac gagttttcgc
    17161 gctacgggcc gcgacagctc ggcattgcgc tcggagacgt gagcgggaaa ggcacggcgg
    17221 ccgcgctctt cggcgccgtg gccatcggca tcctgcgaag cctcgcgcca cagaagctgc
    17281 ggccggcgga attgctgcgc cagatcaatc acctcatcgg cgagcgccgc atcgaagggc
    17341 ggttcatgac catgtgcttc gccacatggg agaagggccg gcaaaaactg cgcgtggcca
    17401 acgccggcca gtcgcagccg cttctctatc ggggcggccg ttgcgacaag atcaatctca
    17461 cggggctccc cctcggaatc tttgaagagg cgaactacga cgaatgggaa tccacgctgg
    17521 attccggcga catcctcgtc ttccattccg acggcatcgc ggaaacggcc aacccggacg
    17581 ggcagttcta cggcacgggg cgcttgcgaa aactggtcga ggaacacgcg caacgagcgg
    17641 cggcggagat cgcagaattg atcctgaggg acgtggatgg gttcgcgcag ggcctgcctc
    17701 tggcggacga ccgcacgctg gtcatcctga aggtacgctg aaagagcgat ggccatccgc
    17761 gcgagcaagg gctctccccg gaaaccgcgg tacgttgatc ccgcatcctt cacgccgcag
    17821 ttttcttgga agcagacaaa gcaaggcgaa gcggtattcg ccgaaggtgt cgccctgcct
    17881 gacgtggccg aaaaggccgg cactcccacg tatgtctaca gccgctccgc aatcgaaagg
    17941 gcctaccgcg aactagacag cggtcttgct ggagttgctc acaccgtgtg ttatgcagtg
    18001 aaagccaaca gcaatctatc gttgctgcgg atactcgcgc gtctcggcgg cggctttgat
    18061 gcggtttcgg gcggcgaaat tgaacgtcta cgccgcgcgg gcatcgcagg caatcgcgtc
    18121 gtattctccg gtgtcggcaa g
//
LOCUS       DASYVO010000330         5467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_263336, whole genome shotgun sequence.
ACCESSION   DASYVO010000330 DASYVO010000000
VERSION     DASYVO010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263336"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VGR03_11205"
     CDS             <1..441
                     /locus_tag="VGR03_11205"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016102133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV1994891.1"
                     /translation="AYKGKVVLLVNVASRCGFTPQYTALESTYEKYKSEGFVIVGIPA
                     NNFGAQEPGTNQEIKTFCSSKFHVTFPMMAKVSVKGEDITPLYQFLTDKAMNPQTGGE
                     IQWNFTKFLIGPDGHVITRFEPDVTPDSPQVTSAIEKALASLKS"
     gene            548..1225
                     /locus_tag="VGR03_11210"
     CDS             548..1225
                     /locus_tag="VGR03_11210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1994892.1"
                     /translation="MPILSLQDVSKDYLTDGQPVHALSGLSLNVERGEFVALVGRSGC
                     GKSTLLNLAGAMDFPTSGKVLLDGVSTSSLKDSGLTQVRREKVGFIFQSFQLLHTLTV
                     FENVELPLLLAGRHNPREAVRERLAWVELEGLGDRYPHQLSGGQMQRVAIARALVHSP
                     SILLADEPTGNLDTTTGNMILELLRRLTREQNTATIMATHSLEAASLANMLVKLRDGK
                     IEEITRR"
     gene            1227..3791
                     /locus_tag="VGR03_11215"
     CDS             1227..3791
                     /locus_tag="VGR03_11215"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1994893.1"
                     /translation="MPLFLLFYRLMVRPLFREPVRTLLTILAVALGVAVVLAIDLAGG
                     AATGSFRSSMETLVGDNDLEVRASGGIPESVVGTLATLPYSIRVSPRIEDYAVIADTK
                     KSLPLIGLDLVAEGSAYAQSESQITAESQTQSASENAFEHLGDADSIWVGSSAGYKTG
                     GRVELLINDRVRDYTVRGVYPDSNGNESAIVMDVASAQHALARYGRVDRILLKVPETP
                     SLEDWQQRLRTVLPAGVEVRPQGTGTNENRRMLAAFRWNLRLLSYISLVVGAFLIYNT
                     ISVSVVRRRPEIGIVRALGASRNVILFAFVGEAACFGLAGALIGLPLGRFMATGAVRL
                     MAATVESLYVSSRPGTIELNVTSVLLALAIGVGVAVASTYAPAREASQVSPVEAMARG
                     RREYDVRVHKARDLWLALVLGLAAAGASRAPAVAGKPLLGYLAAILLVAASALAMPAF
                     VDTLTSLSSKLLGKLLGVEAMLASRSLAASLRRTSVLVGALSTAIAMMTAVGIMVGSF
                     RQSVVLWMSNQLPADLYLRPAGSPAADRHPTISLGLAEEIAKLSGVVAVERLRAYEIR
                     YQGMPATLASADLKVLDVIRWYPHSEFLSGRPQQQVLPELRHSNTVVVSEPFTYKHHL
                     KAGDFITLSLGETQVAFRIADVYYDYSSERGSILMDRSTMLRYLPDPAPSNLAIYVSP
                     EAQLEMVRSEIERATAGHRVLMFSNRDLRAEAIRIFDRTFVITYALEAVAVIVAVMGI
                     AGALLALVIDRRRELGLLRFLGAATGQVRRLILVEAGLLGLLANLAGLALGFALSLIL
                     IYVVNKQSFGWTIRFHWPVEVLLVALTIVYVATVLAGLYPAQVAVRLNPLEVVHEE"
     gene            3781..4869
                     /locus_tag="VGR03_11220"
     CDS             3781..4869
                     /locus_tag="VGR03_11220"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEV1994894.1"
                     /translation="MKSRLACFVAALLLLQPLAAQYRTALPGYRYEFPRDHFNHPDFQ
                     TEWWYYTGNLKSPDGHRFGFELTFFRQAVSRDPAKAAAWEVQDLYLAHLALSDLDGGK
                     FYHSERTNRAGPGIAAVSESLGRIWNGNWQIQWHGNDQELSAVDERFQLHLTLHPEKL
                     PVIHGENGVSQKAEGPGRASHYVSLTCLATSGAIDLGSKTFEVRGTSWMDHEFFTHQL
                     ESSQTGWDWLSLQLEDHTEVMLFHIRRKDGSIDPYSAGTYVDAEGKTTHLRSSDFKLQ
                     PVGEKWRSAVTSAEYPIHWKIAIPKLGLELEVKTPLASQELTGKTKLAPNYWEGAILL
                     TGHRNAQSLSGAGYLEMTGYDRPVQLAP"
     gene            complement(4921..4995)
                     /locus_tag="VGR03_11225"
     tRNA            complement(4921..4995)
                     /locus_tag="VGR03_11225"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4961..4963),aa:Asn,seq:gtt)
BASE COUNT         1081 a         1616 c         1538 g         1232 t
ORIGIN      
        1 gcctacaagg ggaaagtcgt gctcctcgtc aacgtcgcca gccgttgcgg ttttacaccg
       61 cagtatacgg cgctggagtc gacctatgag aagtacaaga gcgaaggctt cgtcatcgtg
      121 ggcatccccg cgaataattt cggcgctcag gagcctggca cgaatcagga gatcaagacc
      181 ttttgctcct cgaaatttca cgtgaccttc cccatgatgg ccaaggtgtc cgtgaaaggg
      241 gaggacatca ccccgctgta tcagttcctg acggacaaag ccatgaatcc gcaaacgggc
      301 ggagagatcc aatggaattt caccaagttc ctgatcggcc ctgacgggca cgtcatcacg
      361 cgcttcgagc ctgacgtgac gccggattcg ccccaggtaa cctctgccat cgaaaaggcg
      421 ctggcctcgc tgaaatcttg agcggagacg gctcgagtca ttccggctgc ggatcttcag
      481 acgcaacgta taatcggtga gctgcaaact gggcgattct gagagtatcg cctgctcgcg
      541 aatccatatg ccgattcttt cgttgcagga cgtttcgaaa gactacttaa cggatggcca
      601 gccagtgcat gcgctcagcg gtctttccct gaacgtggag cgcggcgagt ttgtggcgtt
      661 ggtggggcgc agcggctgcg gaaaatccac gctgctcaat ctcgccgggg ccatggactt
      721 tccgacgtcg ggcaaggtac ttctcgatgg agtttcgacg tcgtccctga aagacagcgg
      781 cctgacccaa gtgcggcgcg agaaggttgg cttcattttt cagtcgtttc aattgctcca
      841 cacgctgacg gttttcgaaa acgtcgaact gccactgctc ctcgccggaa gacacaaccc
      901 gcgcgaggcg gtccgggagc ggcttgcctg ggtggaactc gagggcctag gcgatcgcta
      961 tcctcaccag ctttccggcg gacagatgca gcgtgtcgcc atcgcacgcg cgctcgtcca
     1021 ttcgccgagc atcctgctgg ccgacgaacc taccggcaat ctcgacacca cgacgggcaa
     1081 catgattctg gaattgctcc ggcgcctcac gcgggagcaa aacacggcga ccattatggc
     1141 gacccacagt ctcgaggccg cctcgctggc gaacatgctc gtcaagttgc gtgatggaaa
     1201 aatagaagag atcacccggc gctgagatgc ccctttttct tctcttttac cgattgatgg
     1261 tgcgcccgct cttccgtgag ccggtgcgga ccttgctgac gatcttggcc gtcgccttgg
     1321 gagtcgcggt cgtcctggct atcgatcttg cgggtggcgc cgccaccggc tcgttccgca
     1381 gttcgatgga aacgctggtc ggcgataacg atttggaagt cagggcgtca ggaggcatac
     1441 cggaaagtgt ggttggaaca ctggccacgc tgccgtattc aattcgagtt tcgccgcgca
     1501 tagaagacta cgcggtgatc gctgacacca aaaagtcgct tcccctcatc gggctggatc
     1561 ttgtcgcaga aggcagcgct tacgcgcaaa gtgaatccca aattaccgct gagtcccaaa
     1621 cgcaaagcgc ttcggagaac gcgttcgagc atctcggcga tgcggacagc atttgggtgg
     1681 gctcaagcgc tggatacaag acaggcggcc gcgtcgaact gctcatcaac gatcgagtcc
     1741 gcgactacac ggtgcggggc gtctaccccg attcgaacgg caacgaatct gccattgtga
     1801 tggacgtcgc gtccgcgcaa cacgcactgg cgcgttatgg ccgcgttgat cgcatccttc
     1861 tgaaagtgcc ggagactccc agcctggaag actggcagca gcgcttacgg accgtgcttc
     1921 ctgccggagt ggaagttcgt ccgcaaggca ccggcacgaa cgagaatcgc cgcatgctag
     1981 cagccttccg ctggaacttg cgcctcttga gctacatttc tttggttgtt ggcgcttttc
     2041 ttatttacaa cactatttcc gtctcggtcg ttcgccgccg cccggagatt ggaatcgtgc
     2101 gcgcccttgg cgccagccgt aatgtcattc tcttcgcatt cgtcggcgaa gccgcctgct
     2161 tcgggcttgc gggcgctctc atcgggcttc ccctcggccg cttcatggcg actggtgcgg
     2221 ttcgcttgat ggccgccacc gtggaatcgc tttacgtcag cagccggcca ggaaccatcg
     2281 agttgaacgt cacatccgtt ttgcttgcgc tggcgatcgg agttggagtc gctgttgcat
     2341 ctacctatgc acctgcgcgc gaggcttccc aagtctcgcc agtcgaagcg atggcgcggg
     2401 gccgccgcga atatgatgtg cgcgttcaca aagcccgcga tttgtggctg gctctcgtgc
     2461 tcggtttggc ggcagccgga gcctcccgtg ctccggcagt agccggtaag ccgttgcttg
     2521 gctatctggc cgcgatcttg ctcgtagcag cctcggcgtt ggcgatgccc gcatttgttg
     2581 acactctgac ttcgctttca tcgaaacttc tcggcaagct tctcggcgtg gaggcgatgc
     2641 tcgcctcgcg cagtctcgca gcttccttgc gccgaacttc cgttctcgtc ggcgcactct
     2701 cgaccgccat cgcgatgatg accgcggtcg gcatcatggt cggaagtttc cgccaaagcg
     2761 ttgttctctg gatgagcaac cagttgcccg ccgatctcta tctccggccc gccggttctc
     2821 ccgccgcaga ccgccatcct acgatttctc tcggcttggc ggaagaaatt gccaagcttt
     2881 ccggcgttgt tgcggtcgag cgtctgcgcg cctatgaaat ccgttatcaa ggaatgccgg
     2941 ccacactcgc ttccgccgac ctcaaggtcc tggacgtcat tcgctggtat ccccattccg
     3001 aattcttatc cggccgcccg cagcaacaag ttcttccgga gctgcgccat tcgaataccg
     3061 ttgttgtgag cgaaccgttc acctataaac accatctgaa agcgggagat ttcatcaccc
     3121 tctctctcgg cgaaactcag gtggccttcc gcatcgccga cgtctactac gattactcca
     3181 gcgagcgcgg cagtattttg atggaccgca gcaccatgct gcgctatttg cccgatcccg
     3241 cgccctccaa tctcgccatt tatgtttctc cggaggcgca actggagatg gttcgatctg
     3301 aaatcgaaag agccaccgct ggccatcgcg tgctcatgtt ttcgaatcgg gacttgcgcg
     3361 ccgaagccat ccgcatattt gaccgcacgt tcgtcatcac ctacgcgttg gaagcggtcg
     3421 ctgtcatcgt cgcggttatg gggattgccg gagctcttct tgctctggtg attgaccgcc
     3481 gccgcgaact tggcctgctt cgcttcctcg gcgcggccac cgggcaagtt cggaggttga
     3541 ttctcgtcga ggcaggtttg cttggacttc ttgccaatct tgccggcttg gccctgggct
     3601 ttgcgctttc gctgatccta atctacgtgg tcaataaaca gtcgttcggt tggacgatcc
     3661 ggtttcactg gccggtggaa gtacttcttg tcgcgctgac gatcgtatat gtggcgaccg
     3721 tgctggcggg tctctatccg gcgcaagtcg cggtgcgctt gaatccgctg gaggttgtcc
     3781 atgaagagta ggctcgcgtg cttcgtcgcc gcgctgcttc tcctgcagcc tctcgcggcg
     3841 caataccgca ccgccttacc tggataccgg tacgaattcc cgcgcgacca tttcaatcat
     3901 ccggattttc agacggaatg gtggtactac acgggaaatc tgaaatcgcc ggacggccac
     3961 cggttcggat tcgaattgac cttcttccgg caagccgtca gccgcgatcc cgcgaaggcc
     4021 gccgcatggg aggtgcagga tctctacctc gcgcatctcg cgttgagcga tctggatggc
     4081 gggaagtttt accattcgga gcgtaccaat agagctggcc cgggcatcgc ggcggtgagc
     4141 gaatccctcg gccgcatctg gaatggtaat tggcaaattc aatggcacgg caacgaccag
     4201 gagctaagcg cggtcgatga gcgctttcaa ctgcatttga cgctgcaccc ggaaaaactt
     4261 cctgtcattc acggtgaaaa cggcgtcagc caaaaggcgg aggggccggg ccgcgcctcg
     4321 cactacgttt ctctaacgtg cctcgcgact tcaggtgcaa tcgatctcgg cagcaaaaca
     4381 tttgaagtgc gcggcaccag ttggatggac catgaattct tcacgcatca gctcgaatcc
     4441 agccagaccg gctgggactg gctgagcttg caactcgaag atcacaccga ggtgatgctc
     4501 tttcacattc gccgcaagga tggttcgatt gatccgtact cggctggtac ttacgttgat
     4561 gcagaaggaa agacgacgca cttgcgctcg agtgatttca agctgcagcc tgttggggaa
     4621 aaatggagaa gcgctgttac aagcgccgag tatcccatcc actggaagat tgcgattccg
     4681 aaactaggcc tcgagctcga agtgaaaaca ccgctcgcat cccaggagct gaccggcaag
     4741 acaaaacttg ccccgaacta ttgggaaggc gccatccttc tcacgggtca ccgcaacgca
     4801 caatcgctga gcggcgccgg ctatctggaa atgaccggct acgaccggcc cgttcaactc
     4861 gctccctgac cagggtattt tactaccgtt ttaacttcgt ccagattcag ggaagaagtt
     4921 tggctccccg ggctagattc gaactagcaa cccttcggtt aacagccgag cgctctaccg
     4981 ttgagctacc ggggacttgc acaacacatt tgttttcatt ctaatacagc catttctttt
     5041 gtgctacctc aacaattgaa tcagtgtgag tccaagattg tgattttgcc gtcgctttgg
     5101 aagcattgaa attgccgcac aagtattcga tcgtggaccg aatgtttgta agagtggagc
     5161 gtatcgatct tgggccaaac ttttgagtca tgacggtgtt cggcatcctt acgggaaaga
     5221 gaaatcgact gttacaaact tgggacccaa aggatcgcag attcgacttc cataagaata
     5281 tgcttgacgg atcaactgtg cgtaccctga cgccacgggc cggccgacaa ttgaggtaat
     5341 ccccgtgcca gcagctctcg cgttcttggc aaatccacga cgctgattca ttcaatgtag
     5401 agactgtctc ggccagtgtc tcgtcacagt caaaccggaa aacaaaagcc acatgcattg
     5461 cttatat
//
LOCUS       DASYVO010000331         1777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_122797, whole genome shotgun sequence.
ACCESSION   DASYVO010000331 DASYVO010000000
VERSION     DASYVO010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122797"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VGR03_11230"
     CDS             <1..1031
                     /locus_tag="VGR03_11230"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1994895.1"
                     /translation="EVVARWTGVPVTAIKEEEMSKLLRIEEELHKRIISQDKAISALA
                     RAIRRSRAGLKAPGRPVGSFLFLGPTGVGKTEVARSMASFLFGSDRALIRFDMSEYME
                     KQSVSKLIGSPPGYVGYEEGGQLTERVRRSPYCVILLDEIEKAHPDLFNILLQVFEDG
                     QLTDGLGNTVDFKNTIITMTSNIGARYLERRSNLGFQSANEGATDKKIEDMVLSEVKR
                     LFNPEFLNRLDEVIVFNALNDGDLLQIIDLMIGTMNQNLAQKNISITLTPEASQWILE
                     KTCADRSYGARPLRRALQKHVEDPLSEALIQGEIQCPAVLEVTVSNDALYYRSVNDKL
                     VAATPLSH"
     gene            1284..>1777
                     /locus_tag="VGR03_11235"
     CDS             1284..>1777
                     /locus_tag="VGR03_11235"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEV1994896.1"
                     /translation="MLGRLPLLAQDAASPRTTNPSFPNAPAAAPVPPKPEEKKKTEKP
                     AAEPAAKPVQIKLETPPAATDTKNPRPQPASQAPEPPGQLKLETPQQEEPKPEAPGTA
                     KPAQSTSAPQARKTIIEKIDFRGNRRIPAATLRARMFTHAGDAYDENSLERDFMALWN
                     TGYL"
BASE COUNT          422 a          528 c          506 g          321 t
ORIGIN      
        1 aggaagtggt ggcgcgctgg actggcgtgc ccgtaacggc gattaaagaa gaagagatgt
       61 cgaagctcct acgcatcgag gaggagctgc acaagcgcat catcagccag gacaaggcga
      121 tctccgccct ggcgcgcgcc attcggcgtt cgcgcgcggg actgaaggcg ccgggccgcc
      181 cggtcggctc cttcctgttt ctagggccca ccggcgtagg caaaacggaa gtggcgcgat
      241 cgatggcgtc attcctgttc ggcagtgacc gcgctttgat ccggttcgac atgtcggaat
      301 acatggaaaa acagtcggtc tcgaagctga tcggctcgcc gcccggatat gtcggctacg
      361 aagagggtgg ccagctcacc gagcgcgttc gccgctcgcc ctattgcgtg attctgctcg
      421 acgaaatcga gaaagcgcat ccagacctgt ttaacatcct gctgcaagtt ttcgaagacg
      481 gacaactgac ggatggactg ggcaatacgg tcgatttcaa aaataccatc atcaccatga
      541 cctcgaacat cggggcgcgt tacctggagc gacgctcgaa cctcggattc cagtccgcga
      601 acgaaggcgc gacggacaag aagatcgaag acatggttct gagcgaggtc aaacgccttt
      661 tcaatccgga attcctcaac cggctggatg aggttattgt ctttaacgcc ctgaacgacg
      721 gcgatctgct gcaaatcatc gatctgatga taggcacgat gaaccaaaac ctggctcaga
      781 aaaatatatc catcacgttg acgccggagg ccagccagtg gattttggaa aaaacctgcg
      841 ccgaccggtc gtacggcgcc cggccgctgc ggcgcgcctt gcagaagcac gtggaggatc
      901 cgctttcgga agcgctgatc cagggcgaaa ttcaatgccc cgcggttttg gaagtaacgg
      961 tgtcaaacga cgccttatat tatcggtctg tgaacgacaa gctggtcgcg gccactccgc
     1021 tctcgcatta gagcaacccg tctatcttga aacgacgcgc cgaaatccct caaccaagga
     1081 atctaaccgt tcctatcctc gcagcaaccg gggagggggt tctaaaacgt tcagcttcca
     1141 gccaactgcc ggcgaactgc gggcatcgga atctctatac ctaattttta gctgaggggg
     1201 aaggcaatct tttgaagatc agcggtggcg ctcgcagacg aaaaatctgg aatggattag
     1261 ggttttcttt agcaatccta tgcttgctcg gccgtcttcc gctgctggcg caggatgcgg
     1321 catctccccg gacgactaat ccgtcattcc cgaacgcgcc ggccgccgcg cccgttcctc
     1381 ccaagcccga agagaaaaag aaaacggaaa aacccgccgc ggaaccagcg gccaagcccg
     1441 tccagattaa gctcgagacg ccgccagcgg caaccgacac taaaaatccc cggccgcagc
     1501 ctgccagcca ggcgcccgag ccgccggggc aactgaagct ggaaacaccg cagcaggaag
     1561 aacccaagcc ggaagcgccc ggaactgcga agcctgcaca gagcacgtcg gcgccgcaag
     1621 ctcgaaaaac tattattgaa aaaatcgact ttcgcggcaa ccggcgtatt cctgccgcta
     1681 cgctgcgggc acgcatgttc acgcacgccg gcgacgccta cgatgaaaac tcgctggagc
     1741 gcgatttcat ggccttgtgg aacaccggct atctgga
//
LOCUS       DASYVO010000332         6125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_24062, whole genome shotgun sequence.
ACCESSION   DASYVO010000332 DASYVO010000000
VERSION     DASYVO010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24062"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /gene="aceA"
                     /locus_tag="VGR03_11240"
     CDS             complement(<1..819)
                     /gene="aceA"
                     /locus_tag="VGR03_11240"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEV1994897.1"
                     /translation="MSSNGTNGANGNGNGEKHQPLNYWANDPRWSGITRPYSYSDVVR
                     LRGSIQIEYTLARLGAERLWNLMHTDAYVPALGAITGNQAIEMVQAGLKAIYGSGWQV
                     AADGNTAGDVYPDQSLYPCDSAPNLVKRINRALLRADQIESAEGKKSGMHWFAPIIAD
                     AEAGFGGSLNAYELMKSMIEAGAAGVHFEDQLSSAKKCGHLGGKVVVPTREFIEKLVA
                     ARLAADVCGVPTILIARTDANSAGLLLSDADPRDREFIYGERTPEGFYQFRGGIE"
     gene            complement(941..2551)
                     /locus_tag="VGR03_11245"
     CDS             complement(941..2551)
                     /locus_tag="VGR03_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994898.1"
                     /translation="MATATTQPNPVLAPLPFSNRIVPVNSAPADFDPARDLPAGFLEF
                     LAPLHAALTLRQRALIARRAYALAEAHTGKVPDYLPPSVATTNSWRIELPAWCADQRN
                     QMTGPADDADLVVKMLNSGAPGVMLDLEDSITNSWEYNARGIKNILEALAGRLTYFDR
                     KRNKTVGINSGGTVIFTRPRGLHLHQAGVLPEELLPASLFDVAMVAYQVDFSALKHPL
                     CIYIPKSESADEALWWRDLFQMIARLKSLPTNAIKCMALVESHPLAFQMEEFAWNLRD
                     HIVGLNLGRWDYMASLIHFNLENPEWVLPDRNTIPHNVAFFQNLRNLLPDICHKHGML
                     AIGGMTALYPSREDAELNARALKILAEDKKNEANSLMDGAWTGHPDQNEIAVSQFPAP
                     NQLQARPANGGTHPDLRPIPAGVGKRTLAGTRAAVRTVIRYRNGVLNGKGASLLDGYM
                     EDLATDRIYRLMISQRMRHSDKVEVLGEAGLPIRHTPELIRELFDEELDRLLRENAKN
                     NDAQLAVTLREARGLSEEMIHREEFNPV"
     gene            2912..3703
                     /locus_tag="VGR03_11250"
     CDS             2912..3703
                     /locus_tag="VGR03_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEV1994899.1"
                     /translation="MAGTDTNPATAVQRALNILEAAAQRREGLTNAEISRKLGIPKSS
                     ASYILRTLEKRSYLRREAETGRYRLGLKILSLGGDAQANLDIADVALPFMRTLGEKIH
                     MTVHLAVLDQGEAVYIEKVEAPGFFKVNTWVGRRMFLHSTSVGKCLLAWLPKHEVETI
                     AKQQGLKKRTPKTITSITKLIADLEHVKQSGYAVDDEENSLGARCLGAPIFDTLGNVT
                     AALGASGTLTQTEEANMPRIIEALKESARRISRQLQKSGASGAAP"
     gene            3910..4641
                     /locus_tag="VGR03_11255"
     CDS             3910..4641
                     /locus_tag="VGR03_11255"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEV1994900.1"
                     /translation="MLVIAHRGASGNAPENTLAAFKKAVALGATFIETDLQLSRDARF
                     VAIHDATVNRTTNGQGAVHDLTLAELRRLDAGSWFGSEFAGERIPTLEEILDFSKKND
                     VVFYLEIKPAGSWGGEHALIGALRESGEIPRTVVISFDAAIVLNLRKIEPTLMTGLLY
                     DGQIENPLEKAVAIGARQLVVRGDLVTPAMIAEARKKDLQVVCWTVNHSAHMRMLAVA
                     GVDGIMSDYPDRLVAAMKKETESIS"
     gene            complement(4701..5564)
                     /gene="rsmA"
                     /locus_tag="VGR03_11260"
     CDS             complement(4701..5564)
                     /gene="rsmA"
                     /locus_tag="VGR03_11260"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV1994901.1"
                     /translation="MARQRLGQHFLADLHWREEIARAIRVSPHSTVPLPRDDRHCWIE
                     IGSGHGEMTQHLLATGASVRAIEIDPKFIAGLRHLAKTFPNLNVVPGDILTADLAAIA
                     SGRRIRIYGNLPYYITSPILHHLFTFADLIDEIHIVIQTEVALRVAAQPGTRDYGYLS
                     VVTQFYTRPEFVFEIPRDAFAPPPEVTSALVTLRLPGERAKLSLGSSAPSAAGARTNP
                     SREATSHFLDFVKLCFSKKRKTLVNNLRALAKPDAAREALASLNLRPDARAEQLPVAQ
                     FAALQALLGGS"
BASE COUNT         1323 a         1540 c         2023 g         1239 t
ORIGIN      
        1 ttcgatgccg ccgcggaact ggtagaagcc ttcgggagtg cgctcgccat agatgaattc
       61 acggtcgcgc gggtccgcgt cggaaagcaa aagaccggcg gaattggcat ccgtgcgtgc
      121 gatgagaatg gtgggaacgc cgcaaacatc cgctgcaagc cgcgccgcca ccaatttttc
      181 gatgaattca cgcgtgggca cgacaacttt gccgcccagg tggccgcatt tcttcgcgga
      241 ggaaagttga tcctcgaaat gaacgccagc ggcgcccgct tcaatcatgg atttcatgag
      301 ttcgtaggcg ttcagcgacc cgccgaatcc tgcctccgcg tcggcgatga tcggcgcgaa
      361 ccaatgcatg ccggacttct tgccttccgc cgattcgatt tggtccgcgc gcagcaacgc
      421 gcgattgatg cgcttcacca gattcggcgc ggaatcgcaa ggatagagac tctggtcggg
      481 atagacgtcg cccgcggtat tgccatccgc agccacctgc catccgctgc cataaatcgc
      541 tttcagcccg gcctgcacca tttcaatagc ttggttgccg gtgatggcgc cgagcgcggg
      601 aacgtaggcg tccgtatgca tcagattcca gagccgctcc gctccaagac gcgcgagcgt
      661 gtactcgatc tggatcgagc cgcgcagccg cacgacatcc gagtaagaat aaggacgagt
      721 gatgccggac cagcggggat cattggccca gtagttgagt ggctgatgct tctcgccgtt
      781 tccgttaccg ttggccccgt tggttccgtt ggatgacatg cttgctcctt gtatcgactg
      841 actcacgact cttgatcggc aatccgagaa agactcttgc ttttgcaaac tcctcgttcg
      901 tcggagcaca gcctgaaggc tgtgccactg caaccgttca tcacacggga ttgaactcct
      961 cgcggtggat catctcttca ctgaggccgc gggcttcgcg gagcgtgacc gcgagctgag
     1021 cgtcattgtt cttcgcgttc tcgcgcaaca ggcggtcgag ctcttcgtcg aaaagttcgc
     1081 gaatcagttc cggcgtatgt cgaatcggca agcctgcctc accgagcact tcgactttgt
     1141 ccgaatgcct catgcgctga gagatcatca atcggtagat tcgatcggtg gcgaggtctt
     1201 ccatgtatcc atcgagaaga cttgcgccct tgccgttgag aacgccgttg cggtagcgaa
     1261 tgacggtgcg aacagcggcg cgggttccgg ctagcgtgcg tttgccgact cccgcgggaa
     1321 tcgggcggag atcgggatgt gtgccgccgt tcgcggggcg cgcctgaagt tgattcggag
     1381 cagggaactg gctcacggcg atttcgtttt gatcgggatg tcccgtccaa gcgccgtcca
     1441 tcagcgagtt ggcttcgttt ttcttgtctt ccgcaaggat cttcaacgcc cgcgcgttta
     1501 gctccgcatc ttcgcggctg gggtaaagag cggtcatccc cccgatcgcg agcatcccgt
     1561 gtttgtggca aatgtccggc aagagattgc gcaaattctg gaagaacgca acgttgtgcg
     1621 gaatcgtatt gcggtcgggg agcacccact cggggttttc aagattgaaa tggatcaggc
     1681 tggccatgta gtcccagcgg ccaagattca ggcccacgat gtgatcgcga agattccagg
     1741 caaactcttc catctgaaag gcgagcgggt gcgattcaac cagcgccatg catttgatgg
     1801 cattcgttgg caggctcttg agccgggcaa tcatttgaaa aagatcgcgc caccagagag
     1861 cctcgtcggc ggattcggat ttcggaatgt aaatgcacag aggatgcttc agcgcgctga
     1921 agtccacctg ataagccacc atcgcgacgt cgaacagcga agccgggagc aattcctcgg
     1981 gcagaacacc ggcttggtgt aaatgcaagc cgcggggccg cgtgaaaatg accgtgccac
     2041 cggaattgat gccaacagtt ttattgcgct tgcggtcgaa gtaagtcaaa cggccggcga
     2101 gcgcttccag aatatttttg atgccgcgcg cgttgtattc ccacgaattg gtgatggagt
     2161 cttccagatc gagcatgacg ccgggagcgc cggaattcag catcttcacc acaagatcgg
     2221 cgtcatcggc ggggccagtc atttgattcc gctgatccgc gcaccatgcc ggaagctcaa
     2281 tgcgccagga attcgtggtg gccaccgagg gcggcaggta atcgggcacc ttgccggtgt
     2341 gtgcttcggc caaagcgtaa gcgcggcggg cgatcaacgc gcgttgccgc agcgtaagcg
     2401 cagcgtgcag cggcgcaaga aattcgagaa agcccgcggg cagatcgcga gccggatcga
     2461 aatccgcggg agcggaattg accggaacga tgcgattcga aaagggcaag ggagcaagaa
     2521 ccggattcgg ctgggtggta gcagtcgcca tggtcgagct cagtcctttc cgctgcgaat
     2581 ccgaatgaaa gccagtcgac aaataggaaa ggcaattcga aagccaaagc gtagaaatag
     2641 gccatctcta aagcccattc gctcaaggag atagccgggg cttccgtacc gccgatggat
     2701 tccgtggaaa aacgcggaca aagagtcgag aaatttgcac accatcggcg agttctttcc
     2761 tttccaaacc actctgttca gtatattgaa catgtgttca gacctgtttt ctccttcggc
     2821 cggatcgccg tcaccctcgc agtattgtgt tcagtattct gaacacttat tctatacttg
     2881 ggacgacacc ttttatcgat cggggacggc catggcaggc acggatacca atccggcgac
     2941 ggcggtgcaa cgggcgctga atattctgga agcggccgcg caacgccgcg agggactgac
     3001 caacgcagag atcagccgca agctcgggat accgaagagc tcggcgagct acatcctcag
     3061 gaccctggaa aaacgcagct acttgcggcg tgaagcggaa acgggacggt accggctggg
     3121 tctaaaaatc ctgagtctgg gcggagatgc gcaagcgaac ctcgacatcg ccgatgtcgc
     3181 gctaccgttc atgaggacac tcggtgagaa gattcacatg accgtacatc tggcggtttt
     3241 ggatcagggc gaagcggttt acatcgagaa agtagaggcg ccgggatttt tcaaggtgaa
     3301 tacgtgggtg gggcgtcgga tgtttttgca ttccacgagc gtcgggaaat gtctgctggc
     3361 ctggctgccg aagcatgagg tggagacaat tgcgaagcag cagggactga aaaagaggac
     3421 gccgaaaact atcacctcca tcacgaaact cattgccgac ttggagcacg tgaagcagtc
     3481 gggatacgcg gtagatgacg aggaaaacag tttgggtgcg cggtgccttg gcgcgcccat
     3541 cttcgacacc ctggggaatg taacggcggc gctcggcgcc agcgggacgc tgacgcaaac
     3601 ggaggaggcc aatatgccga ggatcatcga agcactgaag gaatcggcgc ggcggatatc
     3661 gcggcaattg cagaaaagcg gagcatcagg ggccgcgccg taagcggctt cgcgcatcct
     3721 agtgattaca gtgcttctgc cagttagatt caaggtgcat tgtaattgcg agcacggagg
     3781 gatgttgcgc gtgagtgtta gcatagtgcg gtgggcggca ccaggaggag taaaggattc
     3841 tggcaaagag tgatttttcg agagtaatgc cgttggacga atgctgatgg ctacggagag
     3901 agtggcgtag tgctggttat tgcgcatcgc ggggcatcgg ggaacgcgcc ggaaaatacg
     3961 ctggcggcgt tcaagaaagc tgttgcgctg ggagccacgt tcatcgagac ggatttgcag
     4021 ctttcgcgcg atgcgcgttt cgtggccatt cacgatgcca cggtgaatcg caccacgaac
     4081 gggcaagggg cggtgcacga cttgacactc gcggagctgc ggcggctgga tgccggctcc
     4141 tggttcggga gcgagtttgc cggagaacgc attcccacgc tggaagagat tctggacttt
     4201 tccaaaaaaa atgacgtcgt gttttatctg gaaataaagc cggccggttc gtggggcgga
     4261 gagcacgcgc tgatcggcgc gctgcgcgaa tcgggagaga ttccgcggac ggtggtgatt
     4321 tcttttgatg ccgcgatcgt gctgaatttg cggaaaattg agccaacgct gatgaccggg
     4381 ctcctgtacg acgggcagat cgagaatccg ctggagaagg ccgtcgccat tggcgcgcgg
     4441 caactggtcg tgcggggaga tctggtgacg ccggcgatga ttgcggaagc caggaagaag
     4501 gatttgcagg tggtgtgctg gacggtgaat cactccgcgc acatgcgcat gctcgccgtc
     4561 gcgggagtgg acggaatcat gagcgattat ccggaccgac tggtggcggc gatgaaaaaa
     4621 gaaaccgaga gcatctcctg atagcgcgag gcataggcag cgattttcca ttctgaagtt
     4681 ggagagcggt cgagcgtcca tcacgaacca cccagcagag cttgaagagc ggcgaattgg
     4741 gcgacgggga gttgctcggc gcgggcgtcg gggcggagat ttagcgaggc gagggcttcg
     4801 cgggcagcat cgggtttggc gagagcacgc aggttgttca ccaaagtttt gcgtttcttc
     4861 gagaaacaca atttcacaaa atccaggaaa tgagatgtgg cttcgcggga cgggtttgtc
     4921 cgcgcgcctg cggctgaagg agcgctggaa ccgagggaga gctttgcgcg ttcgccgggg
     4981 aggcggaggg tgacgagggc ggaggtgact tccggcggcg gcgcgaacgc gtcgcgagga
     5041 atttcgaaga cgaattctgg gcgcgtgtag aactgggtga cgacggaaag gtagccgtaa
     5101 tcgcgcgtgc ccggttgggc cgcgacgcga agggcaactt cggtctgaat gacgatgtgg
     5161 atttcgtcga tgaggtcggc gaaggtgaag aggtgatgga ggatgggaga ggtgatgtag
     5221 tagggaagat tcccgtagat gcggatgcgg cggccggatg cgatggcagc gaggtcggct
     5281 gtgagaatgt cgccgggaac gacgttgaga ttggggaatg tttttgcaag gtggcgaagg
     5341 ccagcgatga atttcggatc gatttcgatg gcgcggacgg aagcgccggt ggcgagaagg
     5401 tgctgggtca tctcgccgtg gcccgaacca atttcgatcc agcaatgtcg atcgtcccgg
     5461 gggagcggaa cggtggaatg cggtgaaacg cgaatggcgc gcgcgatctc ttcgcgccag
     5521 tggagatcgg cgaggaagtg ttggccgagg cgttggcgtg ccatgggtgg aacagtgtag
     5581 caggggaggt agtgcagaaa ggggataggg gataggggat aggggatagg gaattgggga
     5641 tagtgacccc tcggctcccg atggtcgctc ggggtagaca tgcttcgtcg cagccgaaga
     5701 gaggtccttc gtcgcgatgc tcctcaggac agattccctt atcgggagtc tggttaacca
     5761 taacaccccc ccaccccctg tttttcggaa gtgtggcagc taaaggagtt agcatttctg
     5821 taagtcttgt gtttgccaca cttacaggag agtccagaag tgtggcatct aaaggactta
     5881 cggggctggt ctgtttgcaa ggaagtaact gggccggacg tgaagatatt gaaggagtta
     5941 gaaggaccgc gtggcgggtg cgcatggttc gcaggacacg gaagaatcgt gccgactcaa
     6001 cgaaacaatt atagcatatt ggtaccttat gtcaatgatt acttgtaagt ggtttggatg
     6061 ctgcgggata gcagggtgtc tgtttccggc cgggccggaa tctgcgattg gatgggcatc
     6121 gggaa
//
LOCUS       DASYVO010000333         4830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_189693, whole genome shotgun sequence.
ACCESSION   DASYVO010000333 DASYVO010000000
VERSION     DASYVO010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189693"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VGR03_11265"
     CDS             complement(<1..398)
                     /locus_tag="VGR03_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV1994902.1"
                     /translation="MSFRHSKIVCTIGPATRSPRMIDRLLSAGMDVARLNFSHGTHEE
                     HAKSISMLRAAASEHEKPIAILADLQGPKIRTGPLAGGGPVLLRTGQRFVITTAKVLG
                     DSTRVNTVFTPLPREVHRSDRILLSDGLIE"
     gene            complement(504..1970)
                     /locus_tag="VGR03_11270"
     CDS             complement(504..1970)
                     /locus_tag="VGR03_11270"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1994903.1"
                     /translation="MNWNRMIQVLAGVAALLMAASFPVAAQVAPAGAPGIPGNPSPAG
                     DTSAIAKEAEGWLAELIKINSSNPPGNEQAAAKYVAGVLEKEGLTPELLEVAPGRSAV
                     VARLRSSAVAEPSRALLLVAHLDTVPVDKTRWSVDPFGAVVKDGYLYGRGAIDDKGML
                     AANLAVFISLKRSMARLNRDVIFLATDDEEAGGDASIRMLIAKHWDKFAAGFAINEGG
                     NVFLKNGKVQYIGVQASEKVAANIAVIARGTSGHASQPTKDNPVVHLAAAVAKIGTYS
                     APVHFTSIVRRYFEGLAPLEDDEIGKWIRSMETPDRGEHAQRVVSDASPLWNAMLRDT
                     IAPTVLTAGVANNVIPAEARANLNVRLLPGNQIDAVVNELNKLVNDPQVKLEVQPNAG
                     LAAPPSSLESDFYGVITKVVTREFGGAPALPFQSTWLTDSAQLRLHNVQAYGLVPFPL
                     TQDDLRRMHGDDERIPLGAFDKGVDVLAKIVTEFAVAH"
     gene            2182..3300
                     /locus_tag="VGR03_11275"
     CDS             2182..3300
                     /locus_tag="VGR03_11275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV1994904.1"
                     /translation="MMKVILSVVLLAVLATGLVLPLAKNSVYAQAPTGPQGGAPPPPQ
                     QQQNPTPPPAPKTAQQPPQAGVTIAVDVPIVTLDVVATTQHGDIIPGLKKENFRILDD
                     GVPQTITNFAPTDAPITMVLLLEFSSRGYGGFFAYVGKYWSTALFPSLQQKDWIALET
                     FDMKTRIEVDFTQNKDEVMQGLYHLYFPGFSESNLFDAILETVDRLKDVKGKKSIVVL
                     ASGVDTFSKHTLDQTMKQLRQSDVTIFCVGLGRAYQNFADMNGGMRGTARMDYLQGEN
                     QLKTFSQETGGHSWFPQFAGEMPSVFQDVAAYLRHQYSLSYSPTTGAKDGKFHKIKVE
                     LVAPDGGPLTVLDQKGKKQKFQVYAREGYQAPKGGVGD"
     gene            complement(3465..4472)
                     /locus_tag="VGR03_11280"
     CDS             complement(3465..4472)
                     /locus_tag="VGR03_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEV1994905.1"
                     /translation="MSRTTPAETKLVICVWHPFTEWRPKPAIAEAIRKRWPEMHVLHL
                     PNYDRLAEELPDTDIFVGYSLRAEQLKDAKKLKWIHSTAAGVAQLMYPELRDSGIMVT
                     NPSGIFSVPMAEHTMGLLLALARNFPDSVRQQDRARWSQQEIWDKPQHLTELNGKVLL
                     IVGYGSIGREVAKRAKAFDMRVWGVTRSGEGERANAEKIFSAAKLHEALPGADYVLIA
                     APETAETKHLIGAAEIARMKRGARLINVGRGSLLDEEGLVQALESGALGGAALDVAET
                     EPLPAESLLWKAPNLFLTPHTSGVSDRLWDRQAAILIELLESWFDGRELFNRVDFARG
                     Y"
     gene            complement(4480..>4830)
                     /locus_tag="VGR03_11285"
     CDS             complement(4480..>4830)
                     /locus_tag="VGR03_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEV1994906.1"
                     /translation="ANADMVGHSGKLEAAIKAVEAVDAGLGQIYQALKPRGGLWIITG
                     DHGNAETMIDPKTGGPHTYHTTNPVPLILVSDDDKLRLKPGGSLRDIAPTMLSALGQP
                     RPADMTGTDLRIIS"
BASE COUNT          947 a         1646 c         1183 g         1054 t
ORIGIN      
        1 aactcgatca acccatccga aagcaggatg cggtcgctac ggtgaacttc tcgcggcagc
       61 ggtgtaaaca ctgtgttcac gcgcgtcgaa tcgcccaata cctttgcggt agtgatcacg
      121 aagcgttgcc cggtgcgcag cagcactggc ccgccacccg ccagaggccc ggtccggatt
      181 ttcggtcctt gaaggtcggc gagaatcgca atcggctttt cgtgctcgct cgcggccgcc
      241 cgcagcatcg agatgctttt cgcgtgctcc tcgtgcgtgc catgcgaaaa attaagccgc
      301 gccacgtcca tccccgcgct caataaacgg tcaatcattc gcggcgagcg ggtcgccggc
      361 ccgatggtgc agacaatttt ggaatgacgg aaactcatgc gtcctttgac ggcattgatt
      421 ctagactgct ttcattcttt acgggaaggg tatgaaatag cgacggtctt tttttactgc
      481 tcgcgattcg attcttttct tcgctagtgc gccacggcga attctgtaac gatcttcgcc
      541 agcacgtcca cgcctttgtc gaacgccccg agcggaattc gctcatcgtc tccatgcatc
      601 cgtctcagat catcctgggt cagcggaaac ggcaccagcc catacgcttg cacgttgtgc
      661 agccgcaact gcgccgaatc cgtcagccac gtcgattgaa acggcagcgc cggcgcgcct
      721 ccgaattccc gcgtaaccac cttcgtaatc actccataaa aatccgattc cagcgacgat
      781 ggcggtgcgg ccagtcccgc attcggctgc acctccagct tcacctgcgg gtcattcacc
      841 agcttattca gttcgttcac cacggcatca atctgatttc ccggcagcaa ccgcacgttc
      901 aggttcgccc gcgcttccgc tggaattacg ttgttcgcga ctcctgcagt cagcacagtc
      961 ggcgcaatcg tgtcccgcaa catcgcgttc cacaacggac tggcatcgga aaccacgcgt
     1021 tgcgcatgtt caccgcggtc cggcgtttcc atcgaccgga tccacttccc aatctcgtca
     1081 tcttccagcg gcgccagccc ctcgaaatat ctacgcacaa tcgacgtgaa gtgcaccggc
     1141 gccgaatatg ttccgatctt cgccaccgcc gccgccaggt gcactacggg attgtctttc
     1201 gtcggctgcg aagcatgccc cgatgtcccc cgcgcgatca ccgcgatatt cgccgccact
     1261 ttctcgctcg cctgcacccc gatgtactgc actttcccgt tcttcaagaa gacgtttccg
     1321 ccctcgttga tcgcgaaccc cgccgcgaac ttatcccagt gcttcgcaat cagcatgcgg
     1381 atgctggcat cgccgccggc ttcctcatca tccgtcgcca gaaaaatcac atcgcggttc
     1441 agccgcgcca tcgaacgctt cagcgaaatg aacaccgcca ggttcgcggc cagcatgccc
     1501 ttgtcatcaa tggccccgcg tccatacaaa tatccatcct taaccacggc gccgaacgga
     1561 tccacactcc accgcgtttt atccaccggc accgtgtcca ggtgcgccac gagcaatagc
     1621 gcccgcgaag gctccgccac cgccgagctg cgcagccgcg ccaccaccgc gctccgcccc
     1681 ggcgccactt ccagcagttc cggcgtcaat ccctctttct ccagcactcc cgcaacatat
     1741 ttcgctgcgg cctgttcatt tcccggcgga tttgacgagt tgatcttgat caattccgcc
     1801 agccaacctt ccgcttcctt cgcaattgcc gaggtatccc cggccggcga cggatttcct
     1861 gggatccccg gcgcacccgc tggcgccact tgcgccgcaa ccggaaaact tgcggccatc
     1921 aacaatgccg ccactcccgc caacacctgg atcatgcgat tccagttcac gaatccatcc
     1981 tcctcgtaac aaatggccgc aacccggccg cctcagtaaa cgttttgcat caaatgtgct
     2041 tcatactaac atgcaggtgt gggtgcttgg cctgggcatt gccgtgacgt tgcgtaaatt
     2101 caaccgcttc ttggccggca tgttgtccgc aggcaggaat catcgccagt tcaaagtttt
     2161 tcgattgcta tcgaggggtg catgatgaag gtcattcttt ccgtagttct gctcgcagtt
     2221 ctggcgaccg gtctggtctt gccgctggcc aagaattccg tctatgccca ggcccccacc
     2281 ggcccccagg gcggcgctcc tcctcctccc caacagcagc agaatccaac tccgcctccc
     2341 gcaccaaaaa ccgcgcagca gcctccgcag gctggcgtca ccatcgccgt cgacgttccc
     2401 atcgtcacac tggatgttgt cgccacaacg cagcacggcg acatcatccc tggtcttaag
     2461 aaagagaact tccgcatcct ggacgatggt gtaccgcaga ccatcaccaa cttcgccccc
     2521 accgacgctc ccattaccat ggtcctcctc ctggagttca gcagccgcgg ctacggtggt
     2581 ttttttgcgt atgtcgggaa atactggtcc acggcacttt tccccagttt gcaacagaaa
     2641 gactggattg cgctggagac ctttgacatg aaaacccgca tcgaggtgga cttcacgcag
     2701 aacaaggacg aagtcatgca ggggctctat catctctatt tcccggggtt cagcgaatcg
     2761 aatttgttcg acgccatact tgaaaccgtg gatcgcctga aggacgtgaa gggcaagaag
     2821 tccatcgtcg tgctggccag cggcgtggac accttctcca aacacacgct cgatcagacc
     2881 atgaagcagc tccgccagag tgatgtcacg attttttgcg tcggtttggg ccgcgcctat
     2941 cagaacttcg ccgacatgaa cggtggaatg cgcggcacgg cacgtatgga ctacctccag
     3001 ggggaaaatc agctaaaaac cttttcacag gagaccggag gccattcctg gtttcctcaa
     3061 ttcgccggtg agatgccgag tgtgtttcaa gacgtcgccg cttaccttcg ccatcagtac
     3121 agcctctcct actcaccaac gaccggcgcg aaggacggca aatttcacaa gattaaagtg
     3181 gagttggtcg cgcccgatgg tggcccgcta accgtactgg accaaaaagg caagaagcag
     3241 aaattccagg tctacgcccg cgaaggctat caagccccca aaggcggtgt cggcgactga
     3301 atttcagcca atgtggagca agttttgtta tcgttctggg cctgaacaat ttctattgca
     3361 ctcgcgtagc aagctggaag gttcattccg tgatttcttg gattgcttct tctctgcgaa
     3421 ctctgtgctc tccgtgtcct ctgtgttaaa tccttcttct tttttcagta gccacgcgca
     3481 aaatccactc ggttaaacaa ttcccgccca tcaaaccagc tctccagcaa ttcgatcagg
     3541 atcgccgcct ggcgatccca cagccggtca ctcaccccgc tcgtgtgcgg cgtgaggaac
     3601 aaattcggcg ccttccacaa cagactctcc gccggcagcg gctccgtttc cgccacatcc
     3661 agcgccgcgc cacccagcgc gccgctttcc aacgcctgaa ccagcccctc ttcatccaag
     3721 agcgatccgc gcccgacatt gatcagtctc gccccgcgtt tcatcctcgc gatttccgcc
     3781 gctccgatga ggtgtttcgt ttccgccgtc tccggcgcgg cgatcaacac gtaatccgcg
     3841 cccggcaatg cttcgtgtaa tttggctgcg gaaaaaatct tttccgcatt tgcgcgctcc
     3901 ccttcacccg accgcgtcac gccccacacg cgcatgtcaa acgccttcgc ccgcttcgcc
     3961 acttccctgc cgatggagcc gtacccgacg atcagcagca ctttcccgtt aagctccgtc
     4021 aaatgctgcg gcttgtccca gatctcctgc tgcgaccatc gcgctcggtc ttgttgccgc
     4081 accgaatcag gaaaattcct tgccagtgcg agcagcagac ccatcgtgtg ctccgccatc
     4141 ggcacggaaa aaatcccgct ggggttcgtc accatgattc cggaatcccg cagttccggg
     4201 tacatcaatt gcgccacgcc tgccgccgtc gagtgaatcc atttcagctt cttcgcgtcc
     4261 ttcaattgct cggcgcgcag cgaatagccc acaaaaatgt ccgtatccgg cagctcctcc
     4321 gccaaccgat cgtaattcgg taaatgcagc acatgcattt ccggccaccg ctttcgaatc
     4381 gcctccgcta tcgccggctt cggccgccac tccgtaaacg ggtgccaaac acaaatcaca
     4441 agttttgttt ctgcaggtgt tgtacggctc atggtcaggc tatgaaatga tgcgcaaatc
     4501 cgtgcccgtc atatccgcgg gccgtggctg acccagcgcg ctcagcatgg tcggcgcaat
     4561 atcccgcagc gacccgcccg gcttcaatcg cagcttgtcg tcatcactca ccaggatcag
     4621 cggcacaggg ttcgtcgtgt gataggtgtg cggccctccg gtcttcggat cgatcatggt
     4681 ctccgcattc ccgtggtccc cggtgatgat ccacagacct ccgcgcggct tcagcgcttg
     4741 ataaatctgc cccaggcccg cgtctaccgc ttccaccgct ttgatcgccg cttccagttt
     4801 ccccgaatgc cccaccatgt ccgcattcgc
//
LOCUS       DASYVO010000334         1779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_276085, whole genome shotgun sequence.
ACCESSION   DASYVO010000334 DASYVO010000000
VERSION     DASYVO010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276085"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VGR03_11290"
     CDS             complement(<1..305)
                     /locus_tag="VGR03_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994907.1"
                     /translation="MHATYIRPRYLPQLQNSTASQKRHSLQNFADTALNAAAGFWFLL
                     AGIFRTGVDRVRLSVVQVALHPSKTGGVGNLLVIGGGNRHFGHHGVPRFGGRRDIGLL
                     "
     gene            757..>1779
                     /gene="lptF"
                     /locus_tag="VGR03_11295"
     CDS             757..>1779
                     /gene="lptF"
                     /locus_tag="VGR03_11295"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEV1994908.1"
                     /translation="MRILDRYIVREVSRHALLGLVVFTFVFFVPQLVRLMGLFVRHAG
                     SGAQIFKLFLCIFPGVFTFTIPMATLIGVLLGLGRMSADSEIIALTALGIGRRRILLP
                     VGVLALTGAILTFATTVWLGPLALRTFRSIQADLIASQISFQVQPRVFDERFPGMVLY
                     VNDVSASGTQWHGVFRAETGGESGSRLTLAENAIVIAEPKQGKLELHLQGGTTHEFSR
                     EDADHYSVTAFGQSDWPIEVNGLTPTQPRQLLNSERSLHELLADKGPGWREARVELHH
                     RLAFPIACFVFALVAVPLGAQPRRGGRAAGSLIAVALIAAYYLLFIMGAGLAREGTLS
                     PAEGMWI"
BASE COUNT          336 a          540 c          508 g          395 t
ORIGIN      
        1 agtagaccga tatcacgacg gccgccaaat cggggaacac catgatggcc aaagtgacga
       61 ttgccaccac caatgacgag aagattccca acgccgccag tcttcgaggg atgtaacgcg
      121 acttgaacca cagataggcg aacacggtcg accccagtcc gaaaaatacc agccagcaga
      181 aaccaaaacc ctgcggctgc gttcaaggcg gtgtccgcaa agttttgcag ggaatgccgc
      241 ttttgcgatg cggtgctgtt ttgcaactgt ggcaggtagc gcgggcggat gtaagtcgcg
      301 tgcatgagca cacctccact gttggactat acgcaccggg gcagcggtta gtttcctagc
      361 aattccgccc tcgcccaaca ggagaggccg cgctgaaaac gcggcctctc ctgggattta
      421 tgatgttgtg gattttactt cgagccatta cgatttgagt ctttggtctt tcgggcagtc
      481 gaggcggcta gcttcttggc aggtttggtt tcgtcctttt ctgcgttttt ccctttagac
      541 agttccgctc ggacaaatgc agctgtcttt gcgacatcca ctttgtaccg cattgcagtt
      601 cgagcaagat tggaatcttt cgcaagagat tgcctcggat tatagccggg gcaatacagg
      661 tcggacacaa gagcgcaaac aacgagaaaa cgatggctgc ggcgtcggcg ggtacatgaa
      721 cgccgtcacg accatgctac actcaccgtt cgcgccatgc gcatcctgga ccgttacatc
      781 gtccgcgaag tttcccgtca cgcactcctc ggactcgtgg tctttacctt cgtcttcttc
      841 gtgccgcagc tcgtgcgtct gatggggctg ttcgtgcgtc acgcgggttc tggcgctcag
      901 atcttcaagc tgttcctgtg catcttcccc ggcgtattca cgtttaccat tccgatggcc
      961 actctgatcg gggtgctgct tggcctcggc cggatgtccg cggacagcga gatcatagcc
     1021 cttaccgcac taggcatcgg ccgccgtcgg atactcctcc cggtcggcgt cctcgcgctg
     1081 acaggggcga ttcttacgtt cgcaacgacc gtttggctcg gaccgctcgc gctgcggacg
     1141 tttcgctcca tccaggcgga tttgatcgcc tcacaaatct ccttccaggt gcaaccgcgc
     1201 gtctttgacg aacgcttccc ggggatggtg ctgtacgtta acgacgtttc ggccagcggc
     1261 acccaatggc acggcgtgtt ccgggcggag accgggggag agagcggctc gcgcctcact
     1321 ctagcagaga acgcaattgt aattgccgaa ccgaagcagg gcaagctgga gctgcacctg
     1381 caaggcggaa caacccacga attctcccgt gaagatgcgg accactattc ggttaccgct
     1441 ttcggccaga gcgattggcc catcgaagtg aacggcctga cgcccacaca gccgcgccag
     1501 ctccttaact cagagcggtc cctccacgaa cttctcgctg ataagggccc cggctggcgc
     1561 gaggctcgag tcgaacttca ccaccgactt gccttcccca tcgcatgctt cgttttcgcc
     1621 ctggttgcgg ttcctttggg cgctcagccg cgccgcggcg gccgcgctgc cggatcgttg
     1681 atcgccgtgg ccttgatcgc agcttattat ctgcttttca tcatgggcgc ggggctggct
     1741 cgcgaaggaa cactctctcc ggcggaggga atgtggatc
//
LOCUS       DASYVO010000335        15332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_146233, whole genome shotgun sequence.
ACCESSION   DASYVO010000335 DASYVO010000000
VERSION     DASYVO010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 15332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15332
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146233"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..284
                     /locus_tag="VGR03_11300"
     CDS             87..284
                     /locus_tag="VGR03_11300"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEV1994909.1"
                     /translation="MPHVQITWVEDRTAEQKRKIAERVTNVLIEDGKAKRENIHVSFH
                     DVPSTNYAEAGVLVVDQKHKP"
     gene            complement(369..731)
                     /locus_tag="VGR03_11305"
     CDS             complement(369..731)
                     /locus_tag="VGR03_11305"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HEV1994910.1"
                     /translation="MGGELIGLVAVILGMGVPLGALYTYYRVCKLRSEERLAAIARGV
                     DIPMEPELNQAARSRRAGILLVSGAIGYIATFGLIASIQADRDIWTAAAFGIIPLAVG
                     IGYFLDWSMIRRDMHASS"
     gene            1238..4432
                     /locus_tag="VGR03_11310"
     CDS             1238..4432
                     /locus_tag="VGR03_11310"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV1994911.1"
                     /translation="MNAWKSLWRHGSRSIWKQAFLLLMGLWLGNPLCGQIGASISGTI
                     TDLTGGALAGVSVTVKNLETGAARIITADEAGRYIASSLEVGLYEVRVEKAGFSPEVR
                     TGITLAVGQQAEVDLTLRVGQVQDTVVVTEDSAIVSVTTRDISGLVGERQVKDLPLNG
                     RSYDQLLALNPGILNYTSQRSGGIGTSNSVVGSMFSTSGRRPQENLYLLNGVEFTSAS
                     EINNTPGGVSGQLLGVDAVREFAVVKDSYGAEYGKRPGAQVNIVTASGTNQLHGNVYD
                     FLRNSALDARNFFDQGAIPEFQRNVFGASLGGPLRRDKSFLFGNYEGYRQNLGLSDLT
                     LVPDAASRASAVASVAPLLALWPLANGPELLTSTGAPSGIAEAFTNPVQHIREDFGTV
                     RFDQIFTDRDAFAGVYTVDDSEAHSPTNNPYSLVDIFLREQVASLSETHAFSPRLLNK
                     AIFGFSRGGFYFNGGTTVNLPGWIHPGQPVGAVVVGGGTTLNGASQITNGGTNAGSNL
                     TAVRNLFTASDQVTFTHGRHSFDFGLWFQRIQANDNLIQDQYGQASFTNLQTFLQGQV
                     STYTYAPSSTPLSWRSIEGAFFVEDAIKLKPSVELRIGFRGEFTDGWNEAGGRASNYA
                     FDTAGVIVTQPVVGNSPFTVNNAKFLPAPRVGIAWSPFASKKTVIRAGAGMYYALLDN
                     LSYRLDQNGPYNTVFAVKNIAVLNIAPDAVYTGAKVIPSRVQPDLRTPTVESWTLKIE
                     QQLSPNTSLGVSYIGSHGYHEILSIDANLPSPTICPASPCPATYPAGTLYYPQNAPLS
                     NTALWNTTHWFSEGVSSYNGLAVDVHRRFGHGLQFRGVYTFSKALDDGDNMNTSVATN
                     SPAFVANPLQPKTDYGRASFDIHHAAVLNATYDLPFGRSKSSDGNRWVKKLIGNWQIS
                     GIETLQSGLPFTPQLSYNPSNDGDTRNPVRPSLNPHFTGPVIQGGPNRYFNPTAFIQP
                     LPGTYGNAGRNILQGPGLAETNVSLGKKFSLSDRLDLQFRSEFFNVFNHTNLNTPNPV
                     VYASATGGPSPTAGVITTTSTTSRQIQLGLKLLW"
     gene            complement(4477..4866)
                     /locus_tag="VGR03_11315"
     CDS             complement(4477..4866)
                     /locus_tag="VGR03_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994912.1"
                     /translation="MRSLVGVLVGAALLFVVYHFYLKSMPATDNGTAPTQAISLTGVR
                     SDLLQIAQAERGYIATNGHCTALDELISSNSLSMARTERDGYSYTVECSGGDFTAAAR
                     HLPAPAGSPIRYPNFAIDSSMQVREID"
     gene            5033..5803
                     /locus_tag="VGR03_11320"
     CDS             5033..5803
                     /locus_tag="VGR03_11320"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEV1994913.1"
                     /translation="MNTTAPPLELQAIEKLREEELEEARAIQSVMLPAESLRAGAVMI
                     SHEFQPIAAVGGDFLDYFKLTDGRIGLYLGDVSGKGLPAALYAALAVGTLRGVHKTGT
                     SPHDVLATLNRRLMIRGMPRRHTAIQYALFDPHSHELQISSAGMPGPLHLCADGCRIL
                     ELAGIPPGLFAATSYETLTLRLQPGDSVLFCTDGITDAFGRDGEQFGIERLQELCDAQ
                     RLASPTEFLGRVFAAVENFSCGREQHDDMAAALFHFCG"
     gene            5934..6746
                     /locus_tag="VGR03_11325"
     CDS             5934..6746
                     /locus_tag="VGR03_11325"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEV1994914.1"
                     /translation="MKTKSPYAFGLALLLSFLISIPVDVWAAPQAAGQRAGEVSRVIP
                     AVNIARSGKTITASAKTAVDWQDLVNTQVNGRARVALDDGSVLNVGSDSSMKVVKHDA
                     GAQQTELELTYGKLRTQAQKIAKPDGKFEVRTPAGVAGVVGTDFFVEFANNTMNVIAF
                     EGLVKVCNLAGVCVLVKAGQMTSVRSGDNSGPLPPTQAPLDLLIGTSKDTDVSGQLGV
                     QAVGPHLGTWVTIGIVAGALGVGLGVGLTRGGGTTPVVGKGGGCSPNNPTAC"
     gene            6986..8725
                     /locus_tag="VGR03_11330"
     CDS             6986..8725
                     /locus_tag="VGR03_11330"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEV1994915.1"
                     /translation="MTTSIRSRVILFLCALTLLSAVAQTALAAPPDAQAKRSPLLAAL
                     QAELERSMKTLSALDPPAYYMGYTVTETQRADVSGSNGALLNSNEARNRWLEVSVRTG
                     KYELDNSHKVGERQMASGGPGAPVPIDDDAEVLRRTIWLETDKQYRAASEALIKIKTG
                     KEVKVETAEGRAPDFSREEAHTFIGPQVSIAVERKPWEERVRVYTKAFRESPAIINSI
                     VTFAAQAQNAYQVTSEGTQLQFGQIRYRLELFIQGKATDGMDIDRYYNFDWVEPKDAP
                     DDKAVYAAEATMRKELEGLVAAPINDPTVGPALLTGRAAAVFFHEVFGHRAEGHRQKD
                     VTEGQTFSKKVGEQILPDFLSITDDTTMKKLGKQDLLGYYQFDDEGVPAQRVALVDHG
                     VLKNFEMSRSPLVGFPRSNGHGRRQLGATPVSRQGNLIVQSSKTVTNAQLRAKLIELI
                     KAQGKPFGLLIDDIAGGFTFTGRGQPQAFQVLPLVVYKVFPDGRPDELVRGVDIVGTP
                     LAALTKIVATGDTPEVFNGYCGAESGSVPVAAASPAILTSELEVQKKESSTDRPPILP
                     PPAHDVIKTGGQQ"
     gene            8740..10554
                     /locus_tag="VGR03_11335"
     CDS             8740..10554
                     /locus_tag="VGR03_11335"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEV1994916.1"
                     /translation="MTGLILLLVAMVSVAAAAEQVPQDNDHTMQAMRDEMDRAKTRLE
                     LKIPNIDQPVRPYYVEYRLLDLEVREVVAEFGTILSSTHTRNRFMDVGARVGGYKLDS
                     SNFVSDDGFRGFIGPTGSVGIDRDYDSLRQDLWIATDQAFKEAVETYSRKQAYLSSLA
                     RQSDIDDFSKAEPVQLIEPLQTPDWTGRNWDQEARQTSATLRAFSELHESRVTYYIVY
                     ATEYLLTSEGTEIRQNRHFAAIEAGLSTFADDGVPLNHFYATYAALPAGLPSVEMVRK
                     GLNVAGTELMALRAAPPAQDYTGPVLFEARAAAPMVAQVLGPALNGARPPISFSPVME
                     QMLSGLGGKSDWVSRLGARVLPNGVSVVDDPGAKDFSGTPLIGGYAVDNEGVRAQKVT
                     LVENGNLKSALMSRRPGPDSNQSNGHGRTAFFDAKPTMSNLFFSSAETFSLAELKKKF
                     LDTCRAEKLAYCIVVREMDNPALSLLHQEDFSELLASYGGGAGTGDRLPLVVYRVYPE
                     TGREEMIRGSRIIGLNSRALRNLAGIGNDNFVFNYMQSQINGFAGTALGAFGSAQSGL
                     PASVVAPSLLFEELEVRGARGEAKRLPLLPPPPMSPAK"
     gene            10577..11179
                     /gene="msrB"
                     /locus_tag="VGR03_11340"
     CDS             10577..11179
                     /gene="msrB"
                     /locus_tag="VGR03_11340"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1994917.1"
                     /translation="MNEITRRGLIFGAATVAAYGLLVGVERVCLSAFADTAEPEDDAG
                     PVKIVKFADDGARLGIYSLAKVRKNKSEWKKQLTPLQYDVTRRAATEYAFSGALHDQH
                     APGLYRCIDCDSALFDSKVKFDSGTGWPSFWEAIADDNVREKKDFSAGVLRTEVKCTL
                     CDAHLGHVFTDGPEPTGLRYCMNSAALRFIPRQTSASDKS"
     gene            complement(11236..13650)
                     /locus_tag="VGR03_11345"
     CDS             complement(11236..13650)
                     /locus_tag="VGR03_11345"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEV1994918.1"
                     /translation="MIRRAFLSIVLASLFAACSYAQASAPSSAASVWNALSGPAMDPA
                     KSAHAENVEIIRDRVHFTFTDGTIQFAQPVNGVTFAAVFHGKGRVRVEPPNPLEAQQL
                     KLFTKQDKLDMPFTDATFSFTDGLADEVAKQVKWQASGAANDDFYAKRQKDREDLGES
                     ALPRLLQGVLSADRVRTAYFLADLKIAGKDWVEFHEDALNPEEIGVGRWADVGPVKLH
                     DVWMSFPAGAKTSAEAWKDPRAKDDFSIHAYHINAAVTAGAELNATARVDIEPRLSGQ
                     SVVILDLDSNLRIDSIKDSKGSALSFYQSRENKDRYQSYGNYVAVILAQPLQPGEPQS
                     LEFHYAGKRAIRKAGNGNYFCESSGWYPERANSFSTRADFDLTFHSPKNAVLVATGEK
                     TSETVDGNTRVTTWKSEIPLAVAGFAYGDYKVANDKAGEVAVDIYANREPDDLMAQLQ
                     RLFESGAIQGAVGTLTPSAMAKTMGQEMANTIRLFSLYFGPYPYKHLSVTSLPISYSY
                     GQGWPGLIYLWSASFLDATQRHEIGLQDGVQLTDFFRAHESSHQWWGHRVGWKSYHDQ
                     WLSEGFAEFSGNLYVQYRENMKEYLNRWRKEKELLKTKDVKSHSVESLGPIWMGQRIV
                     SSETDRRSYQDLIYSKGGYVLHMLRMQLNDSRNPDPDHLFKDMMHDYCNTFDNKAAST
                     EDFKAIVEKHMTRGMDLDGNHKMDWFFNQYVYGTGIPQYSFHASLEATPDGKTHIKGE
                     LTRSGVPETWKDAVPLYAHMGDKSVRLGTLAVSHPSEPLDVTLPGKIDRVSINDYEDL
                     LADVKQ"
     gene            13830..15254
                     /locus_tag="VGR03_11350"
     CDS             13830..15254
                     /locus_tag="VGR03_11350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HEV1994919.1"
                     /translation="MEAINQVEEGREKKRAALLSVGSALLLVSLKTFLVVRTESLGVL
                     SEALHSGLDLLAAIITFLSVRMSDQPADEKHPYGHGKFENFSAFVETGLLGLTALYIV
                     YEAFHRLFFRSVHIQPSLTAILVLLVALSIDITRARALGRVSRKYSSDALEADALHFS
                     TDVWSTIVVIAGIGLVWAGETWNLPWLNYADALAGLTVAAVILWVGAQLGRRTLDALL
                     DAAPQGLQQEIAKAVARMDGVLDVDRVRVRRAGNRHFVDATVSVARTASLEQVHALSD
                     AIEKRIGEIVPADVMVHAEPRAPQGEHLFEAIRAVAQKLGLAIHDLAAVQQDGQLFIE
                     LHLEVDENLSLREAHRQSTDLEEKIRELRHGPIEVNIHIEPQGRRIATPDTGVGEMKQ
                     LSRAIEEFLNRLPSEFDELVNCHDVRARQVEHHILVSCHCIMKGDYSITRIHDVLAAL
                     EDLVKERFPQISRVTIHPEPPEAT"
BASE COUNT         3267 a         4340 c         4667 g         3058 t
ORIGIN      
        1 ggctttgcgg gaaccgcggc ggcggataga atgccagctt ccacatgcaa gatatcggga
       61 atcggtcgtt ctaacaagga gggaacatgc cgcacgtgca aattacctgg gtggaggacc
      121 ggacagcgga gcaaaaacgg aaaattgcgg agcgcgtcac aaacgtgctg atcgaagatg
      181 gcaaggccaa acgcgagaat attcacgtct cgtttcacga tgttccatcc acgaattacg
      241 ccgaagccgg tgtgctggtt gtggaccaga agcacaagcc gtgagcaatt gcggggcgaa
      301 atggatcaag gcgggctgcc tcttcgacga actgcggcaa agcccgcccg cgcgtagtga
      361 cgacaggttt agctcgacgc gtgcatgtcg cggcgaatca tggaccagtc aaggaagtaa
      421 ccgattccaa ccgcgagtgg aataatgccg aacgcggcag ccgtccagat gtcacgatcc
      481 gcctggatgc tcgcgatcaa tccaaaagta gcgatgtagc cgatggctcc gctgacgagc
      541 agaatgccag cgcggcggga acgcgccgcc tggttcaatt cgggctccat cggaatgtcg
      601 actccgcggg caatcgccgc gagccgctct tcgctgcgca gcttgcagac ccggtaatag
      661 gtgtacagtg cgccgagggg gactcccatt cccagaatca cggccactaa tccaatcaat
      721 tctccgccca tggtgtgctc cttctttcat ttaggcgccc gtaaggcgcg ttttattggg
      781 ggaaccgctt tcaatgaaag gtacgcggag ccagcagaaa aagttcccgg gatttaggaa
      841 gaaagggggg tagctactct tgcaggggcg ctgatcaggc agtggcggag atggccattc
      901 ttgctttgga gcacaacact gcgacactca ttctgaagtg acgatctgga tccaagcctc
      961 gcgaatgatt gaataatcga atgcgaacca ttcgaaaagc ggcattgaca gaaactatga
     1021 acgttcgtac cgtgtgtaat cagcttgatt atgatgcaaa agcctgactc ttcagtcgtg
     1081 tctctctccc gcagacctgc tctgcgctgt tgttgttgag ccacacccga agtttgtttt
     1141 ctaaccacca aatctagttt ccagtgcgca caggcggaaa ccccagccgc gcataacctc
     1201 gatacatcca agaaaaacca actttcagga gatttcaatg aacgcgtgga aaagtctatg
     1261 gcggcacgga agccgcagca tctggaaaca agcctttctc ttgctgatgg gactgtggct
     1321 tggcaatcct ttgtgcggac agattggagc ctcgatttcc ggaaccatca cggacctgac
     1381 aggcggcgcc ctggcgggcg taagtgttac ggtgaaaaac ctcgagactg gcgcggcccg
     1441 gattatcact gcggatgaag cgggacgcta catcgcgtcg tcgctggagg ttggactcta
     1501 tgaggttaga gtcgagaaag cggggttcag cccagaggtc aggacaggaa tcacgctggc
     1561 cgttgggcag caggcggagg tagatctgac gctccgagtg gggcaggtgc aagacactgt
     1621 ggtggtcact gaagacagcg ccatcgtgag cgttaccacg cgggatattt ccgggctggt
     1681 cggcgagcgg caagtgaagg acctgccgct gaacggccgg agctacgatc agttgctggc
     1741 cttaaatccc gggatcttaa actatacgtc gcagcgttcc ggcgggatcg gaacatccaa
     1801 ctcggttgtc ggaagcatgt tctcgacttc gggccgccgg ccccaagaga atctctacct
     1861 actgaatgga gtggaattca cgagcgcgtc ggaaatcaac aacacgccgg gtggagtgag
     1921 cgggcaactc ctgggagtgg acgcggtccg cgaatttgcc gtcgtcaagg attcctacgg
     1981 cgccgaatat ggaaagcgtc cgggagctca ggtcaacatc gtgacagcct ccggcacaaa
     2041 ccaactccac ggtaacgtct acgattttct gcgaaacagc gccctggacg cccggaactt
     2101 cttcgaccag ggtgccatcc cggagtttca acgcaatgtg ttcggcgctt cgctcggcgg
     2161 gcccttgcgg agagacaaga gctttctgtt cggaaattac gaaggctacc ggcagaatct
     2221 ggggctgagc gacctcacgc tcgtgcccga tgccgcatcg cgggccagcg ccgtagcaag
     2281 tgtcgcccca ttgttggctt tgtggccgct ggctaacggc cctgaacttc taacctccac
     2341 gggagcgccg agcgggattg ccgaagcgtt cacgaatccg gtgcagcaca ttcgcgagga
     2401 ctttggaaca gtgcgcttcg accagatctt tacggacagg gatgcgttcg cgggagtcta
     2461 caccgtggac gacagtgaag ctcactcgcc caccaacaat ccttacagtc tggttgacat
     2521 tttcttgcga gagcaggtcg ctagcctgag tgagactcac gccttttctc cccgtctcct
     2581 caacaaggca attttcggtt tctctcgcgg gggattctat ttcaacggtg gaacgacagt
     2641 gaatctgccg ggctggatcc atccggggca gcccgtcggc gccgtggtgg tggggggagg
     2701 cacaacgctc aacggggctt cgcaaatcac caatggcggc acgaacgcgg gaagcaatct
     2761 cacggcagtg cgcaatctgt ttaccgcgtc cgatcaggtg acgttcactc atggccgcca
     2821 ctcgttcgac ttcggtctgt ggttccagcg gatccaggcc aacgacaatc ttatacagga
     2881 ccagtacggc caggcttcgt ttaccaacct gcaaactttt ctgcaaggcc aggtcagcac
     2941 ctatacctac gctccgtcat ccacgcctct gtcgtggcgt tccatcgaag gcgctttctt
     3001 tgtcgaagat gcgataaagc tgaaaccatc cgtcgagctg cggatcgggt ttcggggaga
     3061 atttactgat ggctggaatg aggctggtgg acgcgcctcg aattatgcgt tcgacacggc
     3121 tggtgtgatt gtcacgcagc cggtcgtcgg caattccccg ttcaccgtca acaatgcgaa
     3181 gttcctgccc gcgccgcgcg ttggaatcgc gtggtctccg ttcgcatcaa agaagacggt
     3241 gattcgagcg ggcgccggta tgtattatgc gctgctcgac aatctgagtt atcgcctgga
     3301 ccagaacggc ccctacaaca cagtctttgc cgtcaagaat atcgcggttt taaacatcgc
     3361 tccggacgcc gtgtacacgg gtgcgaaagt aatcccgagt cgtgtacagc cggacctcag
     3421 gactccgacg gtggaatcct ggacgctgaa aatcgaacaa cagctctctc cgaacacttc
     3481 gttgggggtt agctatattg gttcgcatgg gtaccatgaa atcctttcga ttgatgcgaa
     3541 cttgccaagt ccaacgatct gcccggcctc gccatgcccc gcgacctatc ctgcggggac
     3601 gctctactat ccgcagaacg caccgctgag caacaccgct ctctggaata cgacacattg
     3661 gttctcggag ggcgtcagct cgtataacgg cctcgcggtg gatgtccacc gccgcttcgg
     3721 tcatgggttg cagttccgcg gggtctatac attttcgaaa gcactcgatg atggcgacaa
     3781 catgaatacc agcgtcgcaa caaactctcc cgcgtttgtc gcgaatccgc ttcaaccaaa
     3841 gaccgattac ggccgggctt ctttcgacat ccatcatgcc gcggttctca acgcgactta
     3901 tgaccttccc tttggacgca gcaagtcatc cgatggaaac cgttgggtga agaaactgat
     3961 cggaaactgg caaatcagtg gaatcgagac tctgcagtcg ggtctgcctt tcacgcccca
     4021 gttgtcctac aacccatcca atgacggcga cacgcgcaat cccgtccggc catctttgaa
     4081 tccccatttt acaggtccag tgatccaggg cgggcccaac cggtatttca atcccaccgc
     4141 atttatccag ccgctccccg gcacctatgg gaatgcgggc cgcaacattc tgcaaggccc
     4201 cggactcgcg gaaacaaacg tgtccctggg aaagaagttc tcgctttcag accggctgga
     4261 cttgcagttc cgttccgagt tcttcaacgt gttcaatcac actaacttga acacacctaa
     4321 tcccgtggtc tacgcatccg cgaccggagg accgtccccc acggctggag tgattacgac
     4381 cacttccact acgtcccggc agattcaact aggcctgaaa cttttgtggt gaagcgccgt
     4441 aagtgcgcaa tctgaatcag actcgcggcg cacgcgtcag tcgatttcgc ggacctgcat
     4501 cgaggagtcg atggcaaaat tgggatagcg gatgggggag ccggcgggtg cggggagatg
     4561 gcgcgccgcg gcggtgaaat caccgccgga acattcgacc gtgtaggaat aaccgtcgcg
     4621 ctcggtgcgg gccatggaaa gagaattgga ggagatgagt tcgtcaagcg cagtgcaatg
     4681 gccgttcgtg gcgatgtagc cgcgttccgc ctgggcgatc tgcaggaggt cgcttcgaac
     4741 gcccgtcagg ctgatggcct gcgtcggagc cgtaccatta tccgtggccg gcatgctctt
     4801 caaataaaag tgatacacga caaagagaag ggcggcaccg acaagaacgc caacaagaga
     4861 gcgcataaaa gaattgtggc tcggcccgcg gcgcactaca agcgggactc gcgacgagtt
     4921 gcgttaggaa acaacgtccc ccattttcgc cctgcatccc agccgctctg ttagactcct
     4981 gcctaccgcc gaatttccct ctccggtgta acgccccctt tcggaccgac gaatgaacac
     5041 gactgcgccg ccgctagaac tgcaggcaat cgaaaagctg agagaagaag aactcgaaga
     5101 agctcgcgcg atccaaagcg tgatgctgcc cgcggaatcg ctgcgcgccg gcgcggtgat
     5161 gatctcgcac gaattccagc ccatcgccgc ggtgggcggc gatttcctgg attacttcaa
     5221 actgacggac ggaagaattg gtttgtacct gggcgatgtc tccggcaagg ggctgccggc
     5281 cgcgttgtat gcggcgctgg cggtaggaac attgcgcggc gttcacaaga cgggcacctc
     5341 gccgcacgat gtcctggcga cattgaaccg gcgactgatg attcgcggca tgccccgccg
     5401 gcacacggcc atccagtatg cgctgttcga tccgcacagc cacgagctgc aaatttccag
     5461 cgccggcatg cccggtccct tgcacctttg cgcagacggc tgccgcattc tggagctcgc
     5521 cggcatcccc ccgggcttat tcgccgccac gagctacgag acgctgaccc tgcgattgca
     5581 gccgggcgat tcggtgcttt tctgcacgga tgggatcacg gatgcatttg gccgcgacgg
     5641 agaacagttc ggaatcgagc ggttgcaaga gctttgcgac gcccagcggc ttgcttcacc
     5701 cacggagttt ttgggccggg ttttcgccgc tgtcgagaat ttttcctgcg gtcgcgaaca
     5761 gcatgacgat atggctgcgg ccctgtttca tttttgcggt tgagtgatgc cggcggcgtg
     5821 tgctcacaat agcatgtaga gagtgggcaa agggctaccc gtttggcgtt gacaggcccg
     5881 gcgatctcca gtaacaacga tagatataag ctgtaaaact aggagagtcc tcgatgaaga
     5941 cgaagtcccc gtatgccttc gggctggcct tattgctttc gtttctaatc tcgatcccgg
     6001 tggacgtttg ggccgcgcca caggcggcgg gacagcgcgc cggtgaagtc tcccgcgtga
     6061 ttccggccgt gaacatcgct cgcagcggga aaaccatcac tgcgtcggcg aagaccgcgg
     6121 tcgattggca agatctggtg aatacccagg tgaatgggcg cgcacgcgtc gcgctggacg
     6181 acggttccgt gctcaatgtt ggttcggatt cgtccatgaa ggtggtcaag cacgacgccg
     6241 gagcgcaaca gaccgaactg gagctgacct acgggaagct ccggacgcag gcgcaaaaga
     6301 tcgccaagcc ggacgggaaa ttcgaagtgc gcacaccggc aggcgtcgca ggcgtggtgg
     6361 gaaccgattt ttttgtggaa tttgcgaaca acacgatgaa tgtgatcgcg tttgaaggac
     6421 tggtgaaggt ctgcaacctg gccggagttt gcgtgctggt caaggccggg cagatgacca
     6481 gcgtacgcag tggagataat tcgggacctc tgccaccgac ccaggcgccg ctcgacctgc
     6541 tgattggcac gagcaaggat accgacgtga gcggacagtt gggtgtccaa gccgtaggcc
     6601 cgcacctggg cacgtgggtg acgatcggaa tcgtcgcggg agctctcgga gttggtttgg
     6661 gtgttgggtt gacgcgtggt ggagggacaa ccccagtagt tggaaaaggc ggcggctgct
     6721 cgccgaacaa tccaacagcc tgctgatatt caacgaatca agcagaattg cgcttgcagg
     6781 gccccggcgg atctcaacag ccggggccct ttgtctttcg gaacaaggcg gcgcctgccc
     6841 ggcatgcaac gcaccgaagg aaagagtcgt ccaacaagtg ttacctgaac ggcgaagacg
     6901 cgcgcgatcg gcgctttgtg agcttccgcc cggtggcgta cactgaatgt ttcgcggtcg
     6961 cgtctaattc cgagtgaggc atcctttgac cacatcgatt cgtagtcgcg tgattctttt
     7021 cctttgcgcg ctgactttgc tttccgccgt ggcgcaaaca gcgctggcgg cgccacccga
     7081 tgcccaggcg aagcgttcgc cactgctcgc ggcactgcaa gcggagctgg aacgctcgat
     7141 gaaaacgctg agcgcgctgg accctccggc ctactacatg ggctatacag tcacggagac
     7201 gcagcgcgcg gatgtgtccg gttcgaacgg agcgctgctc aacagcaacg aagcgcgcaa
     7261 ccgctggctg gaagtttcgg ttcgcacggg aaagtacgag ctggacaatt cgcacaaggt
     7321 cggcgagcgg caaatggcca gcggcggacc gggagcgccc gtaccgatcg acgatgacgc
     7381 cgaagttctg cgaaggacga tttggctgga gaccgacaag cagtaccggg ccgcatccga
     7441 agcgctgatc aagatcaaga ccggcaaaga agtaaaagta gaaacggcgg aaggtcgcgc
     7501 gccggatttc tcgcgcgaag aggcgcacac ctttatcgga ccgcaagttt ccatcgccgt
     7561 ggagcgcaag ccgtgggagg aaagagtccg cgtctatacc aaggccttcc gcgaatcgcc
     7621 cgccatcatc aattccatcg tgacgtttgc ggcgcaagcg cagaacgctt atcaggtgac
     7681 cagcgaaggc acgcaattgc aattcgggca gattcgctac cggctggagc tgttcataca
     7741 gggtaaggcc acggatggca tggacatcga ccgctactac aatttcgatt gggtcgaacc
     7801 caaagatgcg ccggacgaca aagcggtcta cgcagcggaa gcgacgatgc gcaaggaatt
     7861 ggaaggactg gtcgccgcgc ctatcaacga tccgaccgtc gggccggcat tgttgaccgg
     7921 acgcgccgcg gcggtgtttt tccacgaagt ctttggccat cgcgccgaag gccaccgtca
     7981 aaaggacgtt accgagggac aaacattttc gaaaaaagtg ggtgagcaaa tcctaccgga
     8041 ttttctgagc atcaccgacg acaccaccat gaaaaagctc ggaaagcagg accttctggg
     8101 ctactaccag ttcgatgatg aaggcgtacc ggcgcagcgc gtggcgctcg tggatcacgg
     8161 cgtgctgaag aatttcgaga tgtcgcggtc gccgctggtg ggtttccccc gttcgaacgg
     8221 ccatggccgg cggcagctcg gcgcaacgcc ggtttcgcgg cagggcaacc tgatcgtgca
     8281 aagcagcaaa acggtaacca acgcgcagct ccgcgccaag ctcattgaat tgatcaaggc
     8341 gcagggcaag ccgtttgggt tgttgatcga tgacatcgcc ggaggattta cgttcaccgg
     8401 acgcgggcag ccgcaggcct tccaggtact gccgctggtg gtttacaaag tttttcccga
     8461 cgggcggcct gacgaattgg tgcggggagt ggacattgta ggcacgccgc tcgccgcgct
     8521 gacgaagatt gtcgcaaccg gcgatacgcc ggaggttttc aacggctact gcggcgcgga
     8581 atcgggatcc gttcccgtgg ccgccgcatc acccgcaatt ttgacttcgg aattggaagt
     8641 tcagaaaaaa gagagttcca cggaccggcc gccgatcctg ccgccgcccg cgcacgacgt
     8701 aatcaaaacg ggaggccagc agtgaggcgc gcagagagaa tgacaggact aattttgctt
     8761 ttggttgcaa tggtgtctgt agcggccgcg gctgagcagg ttccgcagga taacgaccac
     8821 acgatgcagg ccatgcgcga tgagatggac cgcgcgaaga ctcgcctgga attgaaaatt
     8881 ccaaacatcg atcagcccgt gcggccatac tacgtcgaat atcggttgct cgatctcgaa
     8941 gtgcgcgaag tcgtcgcgga attcggcacg atactctcca gcacgcatac gcggaaccgt
     9001 tttatggatg tgggagcgcg tgtcggcggc tacaagctgg atagctccaa ctttgtaagc
     9061 gacgacggct tccgcggctt catcggaccg accggctccg tgggaatcga ccgcgactac
     9121 gattcgctgc ggcaggactt gtggatcgcc acggatcagg ccttcaaaga ggcggtggag
     9181 acttattcgc gcaagcaggc gtatctcagc agcctagcgc ggcaatcgga catcgatgat
     9241 ttctcgaaag cagagccggt gcagctcatc gaaccgctgc agactccgga ttggaccggc
     9301 cgcaactggg accaggaagc gcgccagacg tcggcaacgc tgcgggcgtt ctccgagctt
     9361 cacgaatcgc gcgtaaccta ctacatcgtc tacgcgacgg aatatctgct gaccagcgaa
     9421 ggcacggaaa tccgccagaa tcggcatttt gccgcgatcg aagccggcct gagcacattt
     9481 gccgacgacg gcgtgccgct gaatcatttt tatgccacct acgccgcact ccctgccggc
     9541 ctgcccagcg tggagatggt gcgcaaggga ttaaacgtcg ccggcaccga actcatggct
     9601 ctgcgagccg cgccgcccgc acaagactat accggccccg tccttttcga ggcgcgcgcc
     9661 gcggcgccga tggttgcgca ggtgctcgga cccgcattga atggcgcgcg gcctccgatt
     9721 tccttcagcc ccgtgatgga gcaaatgctc agcggtttgg gaggaaagag tgactgggtg
     9781 tcgcggcttg gcgctcgcgt tttgcccaac ggtgtgtcgg tggtggatga tccgggcgca
     9841 aaggatttca gcggcacgcc gctgatcggc ggttacgccg tggacaatga gggcgtgcgc
     9901 gctcaaaaag tcacgttggt cgaaaacggc aacctgaaga gcgccttgat gtcccgccgg
     9961 cccgggccgg actccaatca atccaacgga cacgggcgca ccgcgttctt cgacgccaaa
    10021 ccaaccatga gcaatctgtt cttttcctcg gcggagactt tttccttggc ggaattgaaa
    10081 aagaaatttc tggacacctg ccgcgcggaa aaactggcct actgcatcgt cgtgcgtgaa
    10141 atggacaatc ccgccctgag cctgctgcac caagaagatt tctccgaact tctggcaagc
    10201 tacggcggcg gtgccgggac tggcgatcgc ctcccgctgg tcgtctaccg cgtctatccg
    10261 gaaaccgggc gggaagaaat gatccgcggc tcgcgcatca tcggattgaa ttcgcgcgcg
    10321 ctgcggaacc tcgccggaat cggaaacgac aatttcgttt tcaactacat gcagagccag
    10381 atcaatggat ttgccggcac ggcgctgggg gcgtttggct cggcgcaaag cggtttgccg
    10441 gcctccgtgg tggcgccgtc gctgctgttc gaggaactgg aagtgcgcgg cgcacgcgga
    10501 gaagccaagc ggctaccgct cttgccgccg ccgccgatga gtcctgcaaa gtaagtattg
    10561 ttcaggaatc gtcgctatga atgaaatcac gagacgcgga ctcatctttg gcgcggccac
    10621 ggttgcagcg tacggtttgc tggtgggcgt ggagcgtgtt tgcctttcgg ctttcgccga
    10681 cacagccgag ccggaagacg atgcgggtcc ggtcaagatc gtgaaattcg cggacgatgg
    10741 cgcgaggctg gggatttact cgctggcgaa agtgcggaag aacaaatccg aatggaagaa
    10801 acagctcacg ccgctgcaat acgatgtaac gcggcgcgcg gcgaccgaat atgctttttc
    10861 cggcgcgctc cacgatcaac acgcgccggg gctgtaccgt tgcatcgatt gcgatagcgc
    10921 gctgttcgat tcgaaagtga aattcgactc cggcacgggc tggccaagct tttgggaagc
    10981 catcgccgat gacaatgtgc gcgagaaaaa agatttcagc gcgggcgtgc tgcgaacgga
    11041 agtcaaatgc acgctttgcg acgcgcattt ggggcatgtc ttcacggatg ggcccgagcc
    11101 caccggcttg cgctattgca tgaattcggc ggcgctgcgg ttcatccccc ggcaaacaag
    11161 cgcttcagac aaatcctgaa gatcctttcg cggaaaaaca aggaccggcc ccaacgcttg
    11221 tcccacctgt agtccttatt gctttacgtc agccaaaaga tcttcgtaat cattgatgga
    11281 gacgcggtcg atttttccgg gcaaggtgac gtcgagcggt tcgctggggt gcgagacagc
    11341 gagggtcccg agacggaccg acttgtcgcc catatgcgcg tacagcggca ccgcatcctt
    11401 ccacgtctca ggcacgccag agcgagtgag ctcgcctttg atgtgggtct tgccgtcggg
    11461 agtagcctcg agcgaagcgt ggaagctgta ctgcgggatt cccgtgccat agacatactg
    11521 gttgaagaac cagtccatct tgtggttgcc gtcgagatcc atgccgcgag tcatgtgttt
    11581 ttccacgatg gctttgaagt cttcggtgga ggccgcctta ttatcgaacg tgttgcaata
    11641 gtcatgcatc atgtccttga aaaggtgatc gggatcgggg ttgcgggaat cgttgagctg
    11701 catccggagc atatggagga catagccgcc tttggaataa atgagatcct gatacgaacg
    11761 gcggtcggtt tcggaggaaa caatccgttg tcccatccag atgggcccga gagattcaac
    11821 ggaatggctc ttaacatcct ttgtcttgag aagttccttc tctttgcgcc agcgattgag
    11881 atattccttc atgttttcgc ggtattggac gtagaggttt cccgagaatt cggcaaaacc
    11941 ttcggaaagc cattgatcgt ggtaactttt ccagccgacg cggtggcccc accactggtg
    12001 ggagctttcg tgcgcgcgga agaaatcagt gagttgaacg ccgtcttgaa gaccaatctc
    12061 gtggcgttgc gtcgcatcaa gaaaggaggc ggaccacaaa taaatcaggc cgggccagcc
    12121 ttgaccgtag gaatagctga tagggaggct ggtgaccgag agatgcttgt agggataagg
    12181 gccgaaatag agagaaaaca agcggattgt gttggccatc tcctggccca tggtcttggc
    12241 catggcggaa ggggtgagag tgccgaccgc gccttgaatg gcgccggact caaagagccg
    12301 ctgcagctga gccatcaagt cgtcaggttc tctgttggcg taaatatcta ctgcgacttc
    12361 gccggctttg tcgttggcga ccttgtagtc cccgtaagcg aacccagcca cggcgagcgg
    12421 aatctcactt ttccaagtgg taacgcgggt atttccgtcc acggtttcgc tggttttttc
    12481 tcccgtggct accaacaccg cattcttcgg gctgtgaaac gtcagatcaa agtcggcgcg
    12541 agtcgagaaa ctgttggcgc gctcgggata ccagccggaa ctctcgcaaa agtaattgcc
    12601 attgccggcc tttcgaatgg cacgtttgcc agcgtagtgg aactccaacg actgtggctc
    12661 accgggttgg agaggttgcg ccagtatgac ggcaacatag ttgccgtagg actgatagcg
    12721 gtctttgttt tcgcgtgact ggtaaaaact cagggcactt cccttggagt ctttgattga
    12781 atcgattctg aggttggagt ccagatcaag aatgacgacg ctctggccgg agagacgagg
    12841 ctcgatgtcg acccgagccg tagcgttcag ctcggcgccc gcggtgactg cagcgttgat
    12901 gtggtacgca tgaattgaga aatcatcctt ggcccgcgga tccttccaag cctcggcgct
    12961 cgtcttggcg cccgctggaa aactcatcca gacgtcatgt agtttcacgg gcccgacgtc
    13021 cgcccatcga ccgacaccaa tctcttccgg gttaagggcg tcctcatgaa attccaccca
    13081 gtctttaccc gcgattttca agtccgccag gaaataggcg gttcgaacgc ggtccgcgga
    13141 tagaactccc tgcaacaagc gcggcagggc ggattcgccg agatcttcgc gatccttctg
    13201 gcgcttggcg tagaaatcat cgttcgcggc cccggaagcc tgccacttca cttgcttcgc
    13261 cacctcgtca gccaagccat ccgtaaagct aaacgtggca tcggtgaacg gcatatccag
    13321 tttgtcttgc ttggtgaaca acttcagttg ctgcgcctca agggggttcg gcggctctac
    13381 ccgcactctc ccctttccat gaaatacggc tgcgaacgtc acgccgttca ccggctgggc
    13441 gaactgaatc gtgccgtcgg tgaaagtgaa gtgcacgcga tcgcgaatga tttcgacgtt
    13501 ctcggcgtgc gcagactttg cgggatccat cgctggaccg gacagggcat tccatacact
    13561 ggctgccgac gaaggggcgg aagcttgcgc gtaactgcag gccgcaaaaa gcgatgcaag
    13621 aacgatcgag aggaaagctc ggcgaatcat atggatgtct cctggttccc agcgagaaag
    13681 tacattgtct tagcttcgca ggcaagtgcc gcgcgataaa cctgaaagat gaggctgaga
    13741 tacgggatct tgaggagata gggaatggcg gcggagggag tgccgcgcgt tcgggagaat
    13801 gaggggagtt gaggtagtct aggcgggcga tggaagcgat caatcaggtc gaggaaggac
    13861 gggagaagaa gcgcgccgcg ctcctgagcg taggttcggc gctgctgctg gtttctctca
    13921 agacgttttt ggtcgtccgg acggaaagct tgggcgtcct ttcggaagcg ttgcactccg
    13981 gcctcgacct gctcgccgcg atcatcacgt ttctctccgt gcgcatgtcc gaccagccgg
    14041 cggatgagaa gcatccctac gggcacggaa aattcgagaa cttctccgca ttcgtcgaga
    14101 ccgggctgct ggggctgacg gctctctaca tcgtctatga ggcatttcac cggctgtttt
    14161 tccgcagcgt ccacattcag ccgagcctca cagcgatcct ggtgctgctg gtcgctttgt
    14221 cgatcgatat aacgcgggcg cgggcgctcg gacgtgtctc tcgaaaatat tccagcgacg
    14281 ccctggaggc cgacgcgctg catttttcta cggacgtgtg gagcaccatc gtggtgatcg
    14341 ccggcatcgg gctggtctgg gccggtgaaa cgtggaactt gccctggctg aactacgccg
    14401 atgccttggc gggcctgacg gttgcagccg tcattttatg ggtgggcgcg cagcttggca
    14461 gacgcacgct ggacgccttg ctggacgcgg cgccgcaagg cttgcagcag gaaatcgcca
    14521 aggccgtcgc gcgcatggat ggcgtgttgg atgtcgatcg cgtgcgggtg cggcgcgccg
    14581 gcaatcgcca ctttgtcgac gcgacggtga gcgtggcgag gacggcgagc ctcgagcagg
    14641 tgcatgcctt gagcgacgcc atcgaaaaac gcattggcga gatcgttccc gccgacgtga
    14701 tggtgcacgc ggaacctcgc gcacctcagg gcgagcactt attcgaagcg attcgcgcgg
    14761 tggcccagaa gttgggcctc gcgattcatg acttggccgc cgtacaacag gatggacaac
    14821 tcttcattga gctgcacctg gaggttgatg aaaatttaag cctgcgcgag gcgcatcgcc
    14881 agtccacgga cctggaggag aaaattcgcg aattgcgcca cggaccgatt gaggtgaaca
    14941 ttcacatcga gccgcagggg aggcgcatcg caacacccga cacgggtgtt ggggagatga
    15001 agcagctttc gcgggcgatc gaggagttcc tcaaccggct gccgtcggag ttcgacgaac
    15061 tggtgaactg ccacgacgtc cgagcccggc aggtcgaaca ccacattctg gtgtcctgcc
    15121 actgcatcat gaaaggcgat tactccatca cgcgcattca cgacgtcctc gccgccctcg
    15181 aagacctcgt caaagagcgc ttcccccaaa tctcccgcgt cactattcac cccgaacccc
    15241 ccgaagccac ctaacaggcc gccttcgctg gcttggaaaa acgtcggtcg agccaggtca
    15301 gcaggaacac gagcgcgagc aggggcacga gc
//
LOCUS       DASYVO010000336         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_54195, whole genome shotgun sequence.
ACCESSION   DASYVO010000336 DASYVO010000000
VERSION     DASYVO010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54195"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..472
                     /locus_tag="VGR03_11355"
     CDS             131..472
                     /locus_tag="VGR03_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994920.1"
                     /translation="MSRPSDNGHDVNLGNCCVCHRARARVIVMLTYKCLKPGTGWGCV
                     QCGLACDGASAIICERCAKTLEQPGSSPIAEKLHFAMLGYAGERRREPIENVWKRERH
                     EHDVSKHADEL"
     gene            502..1134
                     /locus_tag="VGR03_11360"
     CDS             502..1134
                     /locus_tag="VGR03_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994921.1"
                     /translation="MKQSYPLYWPEGWKRTAPGKRVRGQFRRTETRYNSSGNSQRLVL
                     PLTISVSVGRIIEQLGRMEIDRHNVIVSTNVETGLDGTPRSDRRAPEDPGAAVYWRDR
                     RETERSMAIDRYDTVADNLAGIAATLEAMRAIERHGGAEILQRVFRGFMALPEASWRT
                     ILGVGAEASLQDAEGAFRELAHRGHSDKGGNEDMGALVKARDEARKELGH"
     gene            1192..1779
                     /locus_tag="VGR03_11365"
     CDS             1192..1779
                     /locus_tag="VGR03_11365"
                     /inference="COORDINATES: protein motif:HMM:NF019000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEV1994922.1"
                     /translation="MPKTNSKFKIPDSGRDPDSKRPLKNWDEAKAAMEVYRRRDVAER
                     ELRVVMDAQIERATAVYTKAAGPVRASIEALRESLEAFAWGRKKEFLSTESGGPGRTR
                     VHDLVEVGFEMGKAYVDIPEQFEAGALAWLEQEFKDRFVKRAPKIMLAVLSAALKKAE
                     EDRDKRFIARMKARKISRRQDDGFVLKVLEVETIG"
     gene            1818..2120
                     /locus_tag="VGR03_11370"
     CDS             1818..2120
                     /locus_tag="VGR03_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994923.1"
                     /translation="MSKRRFYFLLTIYGLVVVGEGTNAFLARRSLAEAERQIEADAQV
                     QKIDAAELALCGRPGVAPGARPVIALPAVPAEVVFHSARSYRTKWEEQSGLRDGGH"
     gene            2177..2419
                     /locus_tag="VGR03_11375"
     CDS             2177..2419
                     /locus_tag="VGR03_11375"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEV1994924.1"
                     /translation="MILRKLANKSSWITKVEYDPRAKVMQLSTKAGKMYPYQGVPAKL
                     VDEFCAADSPGEFFNERVVGKFELARQKGGKGGASR"
     gene            2416..>2714
                     /locus_tag="VGR03_11380"
     CDS             2416..>2714
                     /locus_tag="VGR03_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994925.1"
                     /translation="MKKTSAKPRKQKGSLRIADSASRIAKAIKEESTKARAVTPRQRT
                     VLANLRKGMSQYKAIRGAGYSEATARHPGELLRGKALGLAFEKLMPSIEELASGIR"
BASE COUNT          591 a          590 c         1055 g          478 t
ORIGIN      
        1 tgaccggctg cgtggcactc gaagccgtcg aacttgcgga agggcgctga tgccagagcg
       61 gagattcaag attccggatt ccgaagactg acaacgggct actctgctga caaccgacta
      121 ctgactatcg atgagccgtc cttccgacaa cgggcacgac gtgaacctgg ggaactgctg
      181 cgtgtgccat cgggcgcggg ccagggtcat cgtgatgctg acttacaagt gcctgaagcc
      241 ggggacgggg tggggatgcg tgcaatgcgg gctggcgtgc gacggggcgt cagcgattat
      301 ttgcgagcgc tgcgcgaaga ctttggagca gcctggcagt tcgccgatcg cggagaagtt
      361 gcacttcgcg atgctgggat atgcgggtga gcggaggcgg gagccgattg agaatgtttg
      421 gaagcgggag aggcacgagc acgatgtgag taaacatgcg gacgagcttt aaagggcacg
      481 ggtcaggaga caggagtcag aatgaagcaa agttatccac tttactggcc ggagggatgg
      541 aagcggacgg cgccggggaa gcgggtgcgg gggcagttca ggcgaacaga aacgcggtat
      601 aactcgtcag ggaattctca gcgcctggtt ttgccgctga ccatttccgt ctcagtgggg
      661 cggatcatcg agcagttagg gcggatggag atcgaccggc acaacgttat tgtctcgacc
      721 aacgttgaga ccgggctgga tgggacgccg cggtccgatc ggagggcgcc ggaggatcct
      781 ggagcggcgg tgtattggcg ggacaggagg gagaccgagc ggtcgatggc gattgaccgg
      841 tacgacactg tagcagacaa cctggcgggg atcgcggcaa cgctggaggc gatgcgagca
      901 atcgagcggc acggcggcgc ggaaatcctg cagcgggttt ttcgcgggtt tatggcgctg
      961 ccggaagcca gttggcgaac gattctgggt gtgggcgcgg aggcctcgct acaggacgcg
     1021 gagggggcgt tcagagagct ggcgcaccgg ggtcacagtg acaaaggggg gaacgaagat
     1081 atgggggcgc tggtgaaggc gcgggatgaa gcgaggaagg agctaggtca ctgagcttgc
     1141 tattctcggc caaagagtgc agggtgataa gtacaggggg cggagagccc cttgcccaaa
     1201 acaaattcaa aattcaaaat tccggattcc ggaagagacc cggattcgaa acgaccgctc
     1261 aaaaattggg acgaagcgaa ggcggcgatg gaagtgtatc gccggcgcga cgtggcagag
     1321 cgtgagctgc gggtggtgat ggacgcgcag attgagcggg cgacggcggt gtatacgaag
     1381 gcggccgggc cggtgcgggc ttcgatcgag gcgctgcggg aaagcctgga agcgtttgcg
     1441 tgggggcgga agaaggaatt tctttccacc gagtcgggtg ggccgggaag gactcgggtg
     1501 cacgacctgg tggaagtggg attcgaaatg gggaaggcgt acgtggacat tccagagcaa
     1561 ttcgaagcgg gggcgctggc ctggctggag caggaattca aagaccggtt tgtgaagcgc
     1621 gcgccgaaga tcatgctggc ggtgctctcg gcggcgctga agaaggcgga agaggaccga
     1681 gacaagcggt tcattgcgcg catgaaggcg cgaaagattt cgcgccggca ggatgatggg
     1741 tttgtgctga aggttttgga agtggaaacg attgggtgag attcaagatt caagaatccg
     1801 aattcgggag gcgggggatg agcaagcgca ggttctattt tttgttaacg atctacggct
     1861 tggtggtggt tggggaaggc accaatgctt tcctggcgcg gcgttcgctg gctgaggcgg
     1921 agcggcagat cgaggctgac gcgcaggtgc agaagattga tgcggcggag ttggcgcttt
     1981 gcggacggcc tggggtggct ccgggagcga ggccggtgat tgctttgccg gctgtgccgg
     2041 cggaggtggt ttttcattct gcgcgcagct accgaacgaa gtgggaagag cagagtgggt
     2101 tgagagacgg cgggcactga ggggaattcc ggattcaaga ttctggattc cggactgacg
     2161 gctgaggaga tcttcgatga tcttgcgcaa gctggctaat aaatcgagtt ggatcacgaa
     2221 agttgagtat gatccgcggg cgaaggtgat gcagctctcg accaaggcgg ggaagatgta
     2281 tccctatcag ggcgtgccgg cgaagctggt ggatgagttt tgcgcggcgg actcgccggg
     2341 ggaatttttc aacgagcggg ttgtggggaa gtttgagctt gctcgacaga aagggggcaa
     2401 aggtggggcg agccggtgaa aaaaaccagc gcaaaaccgc gcaagcagaa ggggagcttg
     2461 cgaattgcgg attccgcatc ccggattgcg aaagcaataa aggaagaaag cacgaaagcg
     2521 cgagcggtga cgccgagaca gcggacggtg ttggcgaatt tgcgcaaggg gatgagccag
     2581 tacaaggcga ttcggggagc gggatactcc gaggcgacgg cgcggcatcc gggtgaactg
     2641 ttgcggggga aggcgctggg gctggcgttt gagaaattga tgccgtcgat tgaggagctg
     2701 gccagcggga tccg
//
LOCUS       DASYVO010000337        21052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_78939, whole genome shotgun sequence.
ACCESSION   DASYVO010000337 DASYVO010000000
VERSION     DASYVO010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 21052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21052
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78939"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VGR03_11385"
     CDS             complement(<1..362)
                     /locus_tag="VGR03_11385"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEV1994926.1"
                     /translation="MIHRHVFRNDQLLPIEKVRLSPGQAGLICGWGIFTTLRISRGEA
                     FAYERHWRRLEKDAALIRLPMPYTGAKVRVHLHEVIRANQVNEGCARIYLVNNQAGFW
                     QSAEPQPAVDLIIYTAGLP"
     gene            537..2114
                     /gene="pruA"
                     /locus_tag="VGR03_11390"
     CDS             537..2114
                     /gene="pruA"
                     /locus_tag="VGR03_11390"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF002852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEV1994927.1"
                     /translation="MATAEKTLHVPEFSNEPFVDFSRTENRKTMEDALRKVASEFGRE
                     YPMYIDGRQVTSVEKRTSTNPSHPSQVIGVFQQASVEVAKQAVEAAHRAFDSWKRVPA
                     EQRAQCLFRAARILRERKFEISALICCEVGKTWPEADADTAETIDFCEFYGREMLRLA
                     GPQKLSPIKGEKNFLVYIPLGVGAIIPPWNFPCAIMGGLVVASLVTGNTVVLKPASDS
                     PTIAAKFVDILYEAGIPKDAINFITGPGGGIGDALISHPRTRYIGFTGSKEVGLRISE
                     LAGKTSPGQIWIKRTVLEMGGKDSIIVDEEADVDSAVEGVAQSAFGYQGQKCSACSRA
                     IVSEKIYDNFLQKLTERTKKITVGPSENPHNYMGPVVSKSAMQTVLDYIEVGKEEGRL
                     LTGGGRAPGDGYFVQPTILADVDPRARIAQEEIFGPVLAVIKARDFDHALEIANNTEF
                     GLTGGVYSRNPAKLEKAAENFHVGNLYFNRKCTGAMVGAHPFGGFNMSGTDSKAGGRD
                     YLLLFLQAKTVAEKMAN"
     gene            complement(2122..3510)
                     /locus_tag="VGR03_11395"
     CDS             complement(2122..3510)
                     /locus_tag="VGR03_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV1994928.1"
                     /translation="MTEKNSSASAAPRNSSGRLLKVLGVWFGIAAAIGNTIAAGIVRT
                     PGDIARWLPNPWLFLGVWVAGGAYALLGASSLAELGAAIPRSGGQYNYSRRALGDYAG
                     FIVGWSDWLSTCGTVAAVAIVIGEYTGALIPALAGQQKQKMVALAVTMGFSLLQWRGI
                     RWGSRAQLLTSAMKTAALVILVLACFALGGSAHRAGAAAAPPTPAMPAGWLLVVALML
                     GMQAVIYTVDGWDGVIYFGEEVKDPGRDVPRAIFGSVFSIMGIYLLLNAAILYVLPMG
                     EIAGNNFALGTASERVFGRFGDPVFRSIMVISLLSAVNACLLFSTRVLHSMSCDGLFF
                     RRAAYVNAGGTPTPALLLSTAAGIVFVLGTFERVIAMLAFFFVANYMLSFASLFVLRR
                     HEPAMQRPYRAWGYPWATGIALVGSVCFLIGAMATDRKNTPLAAGLLVASYPVFRVLK
                     WVSRRAVRPRSS"
     gene            3759..4361
                     /locus_tag="VGR03_11400"
     CDS             3759..4361
                     /locus_tag="VGR03_11400"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1994929.1"
                     /translation="MKLGETLLEQNFVYRLWQAPFASTKLAPVLAHNNLRQVRRVLDV
                     GCGPGTNTHLFAHADYLGIDINPDYIAHARKTFQRSFVLADVTTYEMPPDQRYDFILL
                     NSFLHHIGDSAVRRILSHMRTLLTPDGYIHILDLVLPVRPSIALLVARLDRGDYPRSL
                     TEWRLLFAENFDLVVFEPFPLKALGATLWNMVYCKGKAKP"
     gene            4358..5368
                     /locus_tag="VGR03_11405"
     CDS             4358..5368
                     /locus_tag="VGR03_11405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1994930.1"
                     /translation="MNAGFRLSVAIPIHNEETVLPELLRRTLSVLDQLIGGPHELLFV
                     DDGSTDRTAEILEEVAQKDPRIVVLSLSRNFGHQAALTAALDHVSGDATVVMDGDLQD
                     LPEAIPQFVARFFDRYDVVYAQRTGRKEAWPLRLCYFLFYRMMANLSDIRLPLDAGDF
                     GLLSRRVVEQLRRMPEHHRYLRGMRSWVGFRQTGIPVERSERHSGKSKYGFLRLLKLA
                     SDGIFAFSIVPIRAAALLGAAAIAVAALFALYALFAKIVLHQSPQGFTALLLLITFLS
                     GVLLFFLGVIGEYVGRVYEESKGRPLYIVSRVVGGVSDGPGDLKRESHGHEVGLRSRG
                     VR"
     gene            5408..6136
                     /locus_tag="VGR03_11410"
     CDS             5408..6136
                     /locus_tag="VGR03_11410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1994931.1"
                     /translation="MDPEYGKGYRELYERHWWWRSREVAVLSVLHRHRGSASPARILD
                     IGCGDGLFFRALEEFGEVEGLESSAALISPHGPYRARIHVAPFDARFRSSKEYDLILM
                     LDVLEHLDNPAEALRLAASLLPPGGSLLLTVPAFRLLWTNHDLINHHRTRYRRGTLRP
                     LLKEAGFLVVDQRYWYQWTCPVKLAQRFVEGFFRLPPALPTVPPHWLNTLLYRFSRFE
                     QETLGAWGIPFGSSLMMYCRKANR"
     gene            complement(6271..8085)
                     /locus_tag="VGR03_11415"
     CDS             complement(6271..8085)
                     /locus_tag="VGR03_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994932.1"
                     /translation="MKATYAKRRYVEGNRCPFRRGPNEVARLSLRIAAIAGTPGRVKL
                     EQEGFMNWLVVAICLAIALLMPELGGRWFRSIEDVFSRIAARRSAAILMIFGATIGLR
                     LLLLPLIPVPVPGVHDEFSYLLQADTFAHWRLANPPHPMWVSLETFHINWFPTYSSMF
                     PPAQSFVLALGQLAGHPWIGVLLSCAAMVAAIVWMLQGWMPPRWALLGGIVTLLRLGV
                     TSYWMNSYWGGAVAAAGGALVAGAIPRILRKPRLQDALLLGLGVAILANSRPLEGLIF
                     CVPAAGVLLVWLWENSKVPRKVKMRRVVAPVLAILVLTGAFTLYYDWRLTGNAFLLPH
                     VLNSRNYIPSGLFLWEKPGPPRQYRNLEMMDFYNGVVRSSYDRSWEGVKRVSEMKLEA
                     YDDGFLWTGAIPLLIFAPFVFRDRRMRFLILTFVLAGLGLFLVIWSLPHYAAPVVCVL
                     YALLIQSLRHLRTFRSAGQRVGLGLSRMIFLFLLGTTATCLYDRIRHPDWGWNGDMGN
                     WERAEIGKQLEEKPGKQLAIVRYGLNHEVGEEWVYNSADIDGSKVVWAREMDPEQNLK
                     LLNYYHDRKVWLIEPESVSEQMRPYVPGEVPPVLLRKP"
     gene            complement(8547..8622)
                     /locus_tag="VGR03_11420"
     tRNA            complement(8547..8622)
                     /locus_tag="VGR03_11420"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8587..8589),aa:His,seq:gtg)
     gene            8792..10462
                     /locus_tag="VGR03_11425"
     CDS             8792..10462
                     /locus_tag="VGR03_11425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1994933.1"
                     /translation="MEFQTSHRRIFERQLTFARPIIILLALLALLDRPLSVTSGRAFY
                     FLLGYLLFSVALVASHRLFERRDLFLPLVVDLAALLFFLYVSPSLTAVWFPFLYVAFA
                     AGGRWGSRAAVFLLGALSLALVFRAVIQGELHLMHIIGWLGLLGATFAAGVALALLGD
                     LSRRAAAENDFLTRITTTLQVDQGLAESFRLFLDELARAFDAAQSLIVFRDLELERIF
                     LWRVVVGQTERISPENLPLARADGFLLDDMDADVCWNSLEGPGQGFGWDRRNGRRIKF
                     LPHLPGPSQHELQVRSLMTVAFDQSGQPAGRLFVVNPRRPCTRRDLRWLDRIARHFSA
                     PAENLFLLRHMRARAIEGERSRISRDLHDGILQTLLSIEIQLDVLRRRLPVAPDQAVA
                     GLAALQQTVKNESAELRALVTDLRPLRVQSADLVDLMRGHAERLRNESFLALELLIDS
                     TEIEAPDHVCRELFQIYREALSNVKKHAKASHVVVKLTQDDSRIILVVDDDGEGFSFA
                     GQFSGDELDRLRLGPISIKERTRAVGGMLTVESSPGHGARVTVEIPLA"
     gene            10464..11129
                     /locus_tag="VGR03_11430"
     CDS             10464..11129
                     /locus_tag="VGR03_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1994934.1"
                     /translation="MAKNKQQIRVLMADDHAIFRDGLRKLLDADDDLTVVGEAQNGAE
                     CIRMLGKLKPDILLLDLRMPDKDGLAVLEEVNFDSLPTRVIVLTAAEDDRDVVRAMRL
                     GARGVVLKQSATDLLVKSIHRVHAGEIWLDNRMTAEVMKAFAKSSEAGPRREKPLLSD
                     REKEIVQLVAQGFRNKEIGEKLFISEQTVKNHLHNIFDKLGVSDRLELALYAIHHRLI
                     DHA"
     gene            11400..11738
                     /locus_tag="VGR03_11435"
     CDS             11400..11738
                     /locus_tag="VGR03_11435"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEV1994935.1"
                     /translation="MAGSERRTTQRFQMRLPLTVRWTTGAAIGETSTESRDVSSRGVY
                     FSLSKDVKEGSPVEILLTLPNEITLAGPVKVRCLGRIHRTEVRDEATVGVVAVIERYE
                     FLRSEEEEAN"
     gene            complement(11821..11967)
                     /locus_tag="VGR03_11440"
     CDS             complement(11821..11967)
                     /locus_tag="VGR03_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994936.1"
                     /translation="MEILFYPKGPGNSQVTIDHRDLKSARAVQEMKVYWSEQFARLET
                     LLKK"
     gene            12205..13017
                     /locus_tag="VGR03_11445"
     CDS             12205..13017
                     /locus_tag="VGR03_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994937.1"
                     /translation="MGKMRLFEIHDQAWFPGFLRDQTTDALQFILNTGNLYGPIVHQL
                     SGALAESGTVRILDLCSGAGGPWLTLSKALEQERGLPLHICLTDKYPNVKTSNTAKSL
                     AGNGVRFHSIPVDATQIPDELNGFRTIFSSFHHFRPWEARAILQDAVNKQRGIGIFEV
                     AGRSAVTVLLTFLMPIGSLLLTPFMRPFRWSRFLWTYILPVIPFVLWFDGIMSCLRAY
                     SLKELIELTSGLTVHGYEWKIGQERNGLLPIPITYLIGYPIRETRESWDVRA"
     gene            13366..16677
                     /locus_tag="VGR03_11450"
     CDS             13366..16677
                     /locus_tag="VGR03_11450"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEV1994938.1"
                     /translation="MKRLNKFCILSGLLVALTGTLSAYAQVDYATATLRGTVLDPQGA
                     IISGASITVTNSSKGISRTVKTAGDGTYQVPALPPGAYQISVEANGFSKEVVKALELT
                     VGQSAIYDFHLKVGNASEIVEVTADNAALIQTDQTQQANTINSLQVEELPNINRSFTY
                     DVYTVPGVSNSEAPRSQNPGFTGYLTTGFSIGGSNGRNNLSTIDGGENEYGTGQYRVT
                     ALPVDAIQEYQVNRNAFAAEFGFTDGSAINIVTKSGGNQWHGDAFGHFRDQRTEATNF
                     FNGGPTKAFSQNVYLGGSLGGPIVRDKLFFFTAYEFQRLDTPNFGNAGLLTQPTVLGL
                     NAPGLGANCAGQFASKTPDQLCYINALKSSGNPFLVGFANGITPGLSPLNNPALNTIL
                     TRDNGVFDSPDRLHNAIMRFDYQASGKDSLSMRAGYVHNNFTSPLIGITNGTPDSYSL
                     FVRDFSILTTWTRSISTNWVNQVLVQIVPRNSSEAIPLHNNGINFSLGNLGAPGPGGT
                     STFGGPALLPYKAHQRRYQFEDNVTWTRGAHTFKFGASYRPADYHVENDLWFNPEFDF
                     RDGIIPLISLAPPAVQAQLVGFNLAHGLPATGPVTTNLTAPQSFAFGIPADVIAGFNN
                     PKWEGWGHYFGSYVQDSWKLNSRLTMNAGVRLDFDGEPPPLGGSFYAAPRVGFAWDPF
                     GDHKTVFRAGAGIYVAPVDVLIPSYGSLLDGTGRYINQVLVPLSPTDPRVAQLWGLGL
                     AKGELPFGQLTPADYAAVGIDITSPGATVGYSVAPNYKNPYSLQASLSIAQQLAKNLS
                     LELGYNMYHGVHLQTPVETAYAEIPVGSPFCPTPACTDATGGPLYRPLTGQFQHTTYA
                     SNGSSIYHGFTASLTRRFSHGLQFQANYTWSKTIDDTIDFSSFQNSFRPSRLNLFRSI
                     SVFDVPHIFVANAVYRLPFKAGEGKPLSSILADITLAPILTMHSGLPFSVRTPSLVNK
                     INGQALDNNYATPFLAPRDGNRGAAFYSFDLSFQKALYIARDRGVRLNLIVQGTNLLN
                     HVNFNHVNDVFDLNGIPASGIVQTGTGPVNLLTGPFTGLRAAVPTNAGQLTQPLFYSQ
                     ADVPRQIQFGLRLAF"
     gene            complement(17021..17097)
                     /locus_tag="VGR03_11455"
     tRNA            complement(17021..17097)
                     /locus_tag="VGR03_11455"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17061..17063),aa:Pro,seq:tgg)
     gene            17348..20674
                     /locus_tag="VGR03_11460"
     CDS             17348..20674
                     /locus_tag="VGR03_11460"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEV1994939.1"
                     /translation="MLAVLFLVAGTARGQGVGASGDIRGTVTDPTGAVVSKATVTVTD
                     AEKGIKRSAVTDPDGEYRFTALPPTTYDISVQFSGFQTYIQKGVVVNVGQTVTLDFHL
                     TVSRAAESVEVTTEAPVVDTQQSAQANVIDTQTIQDLPINRRDYLTFSLLAPAVTDST
                     RLAGDQDFRVKQTPQSGLSFYGSNGRGNSVTVDGGEVNDDAGGVRLTLSSEAVQEFQI
                     NRSNYGPDLGGASGASINIVSKSGSNQVHGTVFGLFRNDAFDERNPFAFTQPLAPGAT
                     FNPALPDVAGQPTQDSLSRYQFGGSVGFPISRDKTFAFVAAEGLISNAQNAVPLFTNT
                     NVFRPTAAQQPIITGLATLAGNPAVPCLTGQPALPAATCAVILTSIFTINPAAANPGT
                     RARNQFLINQFEANGGLFAYDTREYFISGRLDHQFSERNQAYLRYSFAHDNEQSPDVQ
                     SLVGFSAGSSIAPAYDNTLQIAWFHQFSPRTLNEARVQWNYSHFDVIPNAPGTVGLNM
                     PGFGFLNSNIFLPSKTIMRRYEFADNFSMVRGHHSLKFGGYELIRGNHTESDTFFPGR
                     FQFGSLPGGIVTPCFQVPAACGLTGVNAATISSLQSFSLGLPQFYQQGFGSAIYAINR
                     PWTAFFASDSWSLRSNFTFTFGVRYELDSQYGRLKTDTNNFAPRIAFAWDPFSDHKMA
                     VRGGYGIFYSSIYGQIPNVIQTLGLVNGFQQIAQVFVPLTGEPGNPALTSAAIFQTLF
                     AQGKVQCTAPAAGNPACITPADLAQFGITIKHDGTAPLSVFFSGQPNYQNPYSQQAEL
                     SVERQIGKSFSVSVSGVYVHTIGLPTSVDGNALPAPITNVPLANGSIVSVRNWNSRLP
                     NPLNSAPCAGATIVNCFVSPTTLQNNFYSSRGAAIYEGGILEIKKRFSDHMSLMFNYT
                     FSKAFSSSTDFNSDFGPQDNTNLVLERGLSDFDQRHKLVIAAVLESPWKNRFFSGFQV
                     APIVRYNSGHPFNLLAGTDVNGDRHSTNDRPIGVAHNTGVGPDFATFDMRVTKRFKFT
                     EKTILSIIAEGFNIFNQINYASVNNVVSPNLLLPTAAGGLGFTTANLHGSSAIAPNQP
                     LGFTSAFPTRQLQLGAKLTF"
     gene            complement(20849..>21052)
                     /locus_tag="VGR03_11465"
     CDS             complement(20849..>21052)
                     /locus_tag="VGR03_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994940.1"
                     /translation="WLIGRGLTFGANIYLHRQDLPSVQLASVPWWMVGGAIAFSVAVS
                     LAAGIFPASRAARLNPVEALRYE"
BASE COUNT         4184 a         6793 c         5708 g         4367 t
ORIGIN      
        1 gggagccccg cggtatagat gatcaagtcc accgccggct gaggctctgc gctctgccag
       61 aagcccgcct gattattgac gagatagatg cgcgcgcagc cttcgttcac ttggttggcg
      121 cggatcacct cgtggagatg cacgcgaact ttcgccccgg tgtaaggcat cggcaggcgg
      181 atgagagccg catccttttc gaggcgtcgc cagtggcgct cgtaggcgaa ggcctcgccg
      241 cgggagatgc gcagcgtcgt aaaaatgccc catccacaga tcaatcccgc ctggccgggg
      301 gaaagccgga ccttctcgat tggcagtaac tgatcgttgc gaaagacgtg gcggtgaatc
      361 acgcgggcct cgctggaatc cctttcgcgg ctattgtatc cgagaagccc tgtcctgtgg
      421 aggcaaggaa gctgggaaac acgcggtgag cgagtgggaa cgaatatgcg cgccttgtag
      481 cggttcctga ccgcgcagta caattccttg ttcgcgcaat caggaggcat ctggcgatgg
      541 ccacggccga gaaaacgctg catgttcccg aattctccaa cgaaccgttc gtggacttct
      601 caaggaccga aaaccgtaaa accatggaag atgccctgag gaaagtcgcc tccgaatttg
      661 gccgcgaata cccgatgtat attgacggcc ggcaagtcac cagcgtggaa aaacgcacgt
      721 ccaccaaccc ttcccaccct tcgcaggtca tcggcgtctt tcaacaggca agcgtggaag
      781 tggcgaagca ggcagttgag gcggcccaca gggccttcga ctcctggaag cgcgttcccg
      841 ccgagcagcg cgcccagtgt ctcttccgcg ccgcccggat ccttcgagag cgcaagttcg
      901 aaatctccgc gctcatctgc tgcgaagtgg gtaagacctg gccggaagcc gacgccgaca
      961 cggccgaaac catcgatttc tgtgaattct acggccgtga aatgcttcgc ctcgctgggc
     1021 cacagaaact ctcgcccata aagggcgaga agaactttct cgtctatata ccgctcggcg
     1081 tcggggcgat catcccgcca tggaattttc cctgcgccat catgggcggc ctcgtggtgg
     1141 cttcgctggt caccggcaac accgtcgtcc tcaagccggc cagcgactcg cccacgatcg
     1201 ccgcgaaatt tgtggatatt ctttatgaag ctggcatccc gaaagacgca atcaatttca
     1261 tcaccggccc cggcggcggt ataggcgacg ccctcatttc tcatcccagg acgcgctaca
     1321 tcggtttcac cggttccaag gaagtcggcc tccgcatcag tgagcttgcg ggaaaaacct
     1381 cgcccggtca gatctggatc aagcgcactg ttctcgagat gggtggcaag gattccatca
     1441 tcgtggatga agaggctgat gtggattccg ccgtcgaggg cgttgcccag tccgccttcg
     1501 gttatcaggg acagaaatgc tcggcctgct cgcgcgccat cgtctcggaa aaaatctacg
     1561 acaactttct ccaaaagctc actgagcgca cgaagaaaat caccgtcggc ccgagcgaga
     1621 atccccacaa ttacatgggc ccggtcgtca gtaagtccgc gatgcagacg gttctcgatt
     1681 acatcgaggt tggcaaggaa gaaggccggc ttctaaccgg tggtggtcgc gctcccggtg
     1741 acggttactt cgtccagccc accatccttg cggatgtaga ccccagggcg cgaatcgcgc
     1801 aggaggagat cttcggcccg gtcctcgctg tcatcaaggc gcgtgacttc gatcacgcgc
     1861 tcgagatcgc caacaacacc gaattcggcc tcaccggcgg cgtctattcc cgtaaccccg
     1921 cgaagctcga gaaggccgcg gagaactttc acgtcggaaa tctctacttt aaccgcaagt
     1981 gcaccggagc gatggtcggc gcgcatccct tcggcggttt caacatgtcc gggacggact
     2041 ccaaggcggg cggccgcgat tacctcttgc tctttctcca ggcgaagacc gtcgccgaaa
     2101 agatggccaa ctaacggcct cctaggatga acggggtcgt accgctctcc gcgagaccca
     2161 cttcaacacg cggaaaaccg gataactcgc caccagcagc ccggccgcca gcggtgtgtt
     2221 ctttcggtcc gtcgccatcg ccccgatcag aaaacacacc gaccccacca gcgcgattcc
     2281 ggtcgcccac ggataacccc atgctcgata ggggcgttgc attgccggct cgtgccgccg
     2341 caggacgaaa agcgacgcga acgacagcat atagttggcc acaaaaaaga acgccagcat
     2401 cgcgatcacc cgttcaaacg tccccagcac aaacacgatc cccgcggccg tgctcaacag
     2461 aagcgccggc gtcggtgtac cgcccgcgtt cacgtacgct gcccgccgga agaacagtcc
     2521 gtcacagctc atggagtgca gcactcgggt actgaataac aggcacgcgt ttacggcgct
     2581 caacagcgaa atcaccataa tcgagcggaa gaccgggtcg ccgaatcgcc cgaagacccg
     2641 ctccgatgcg gttcccaggg caaaattgtt tccggcaatc tctcccattg gcagcacata
     2701 aaggatggcc gcgttgagca ggaggtagat tcccatgatc gaaaacacgc tcccaaatat
     2761 tgcgcgcggc acatcccgtc cgggatcctt cacctcttcg ccgaaataaa tcacgccgtc
     2821 ccagccgtcg accgtataga ttaccgcctg cattccgagc atcagcgcga cgacaagcag
     2881 ccaaccggct ggcatggcgg gtgtcggtgg ggccgcggcg gctcccgccc tgtgcgcgct
     2941 tccgcccagc gcgaaacacg cgagcacgag gattaccaag gccgccgtct tcatcgccga
     3001 cgtcagaagt tgtgcccggc tgccccagcg aatgcctcgc cattgcagta atgaaaaccc
     3061 catcgtcact gccagtgcga ccattttctg tttctgctgt ccggccagcg ccggaatcag
     3121 tgcgccggtg tattccccaa tgacaatcgc caccgctgca accgtcccgc acgtcgaaag
     3181 ccagtcactc catccgacga taaaaccggc gtagtcaccc agcgcccgcc gcgagtaatt
     3241 gtattgcccg cctgagcgtg gaatcgccgc gcccagctcg gccagcgatg aagcacccag
     3301 cagcgcatac gcgccgcccg ccacccacac gcccaggaac agccacggat tcggcagcca
     3361 tcgagcgatg tcgccgggcg tgcggacgat tcccgccgca atagtgtttc cgatggccgc
     3421 ggcaattcca aaccacacgc ccagcacttt caacaatcgg cccgacgaat tccggggagc
     3481 ggctgatgca gatgagttct tctcagtcaa gcgtttgcgc ccgggattta atagaaagag
     3541 ccgccatcat aaccgcggcc ttggcgcttg caaaccgaaa attgcttgcg gcaaggagtt
     3601 ccatcgtcag cgccacggtc atccgccttc acgccaaatt cattcgctga acacaaccca
     3661 ctcctccccg gccttctctt tttgtggcgg acatgatgca atcattcgcg ggggcagggc
     3721 gtgcagcgaa tcagctcctt tgaggtacgc tggcatctat gaagcttggt gaaacacttc
     3781 tcgagcagaa ctttgtctat cggctttggc aggcgccttt cgcctcgaca aagctggcgc
     3841 ctgtgcttgc acataacaat ctccgccagg tgcggcgcgt gctcgatgtg ggctgcggtc
     3901 ctggcacgaa tacgcatctc tttgcgcacg ccgattacct cggcattgac atcaatccgg
     3961 actacattgc ccacgcacgt aagacattcc agcggtcatt tgtccttgct gacgtcacga
     4021 cctatgagat gccgccggac cagcgctacg acttcatcct gctcaacagc tttctccacc
     4081 acatcgggga ttctgccgtt cggcgcatcc tctcccatat gcgcacgctc ctcacgccgg
     4141 atggttacat tcacatcctc gacttggttt tgccagtccg cccgtcaatc gctctgctgg
     4201 ttgctcggct ggaccgtggc gattacccac gctcgctcac ggaatggcgg ctactcttcg
     4261 ccgagaattt cgaccttgtc gttttcgagc cttttccgct caaagcgctg ggcgcaacgc
     4321 tctggaacat ggtctattgc aagggaaagg caaagccatg aatgccggat ttcgtctctc
     4381 cgtcgccatc cccattcaca acgaagagac cgtcctgccc gagcttctgc gccgcacact
     4441 ttcggttctg gaccagttga tcggtggccc gcatgaactc ctcttcgtgg atgatggcag
     4501 caccgatcgc accgccgaaa tcctcgaaga ggtggcgcag aaagatccgc gcatcgtggt
     4561 tctgtcgctt tctcgcaatt tcggccatca agcggccttg acggccgccc tcgaccatgt
     4621 cagcggagat gctaccgtgg ttatggacgg tgatctgcag gatcttccgg aggccattcc
     4681 tcagttcgtt gcgcgcttct tcgatcgcta cgacgttgtc tatgcccaac gcactggccg
     4741 caaggaggcc tggccgctcc gcctctgcta tttcctcttt tatcgaatga tggccaatct
     4801 ttcggacatc cggcttccat tggacgccgg ggatttcggc ctgctgtcgc ggcgcgtggt
     4861 cgagcagctc cgacgaatgc ctgaacatca ccgctacctt cgcggcatgc gaagctgggt
     4921 cggtttccgc cagaccggaa ttccggtgga gcgctccgag cgtcattccg gcaagagcaa
     4981 gtacggcttc ctgcgccttc tgaaacttgc ctccgacggt atctttgcct tctccattgt
     5041 tccgatccgc gcggcggccc tgctcggcgc ggcggccatt gcagtcgctg ctctcttcgc
     5101 gctctatgcg ctgtttgcga agatcgttct acaccaatct ccgcaaggct ttaccgcgct
     5161 acttctgctc atcacctttc tctccggagt tctgctgttc tttctcgggg tgatcggcga
     5221 atacgtgggc cgcgtctacg aagagtccaa aggacgcccc ctgtatatcg tgagtcgggt
     5281 tgtcggcggc gtgtccgacg gcccgggcga cctcaagcgc gagtcccacg gccacgaagt
     5341 cggcttgcgg tctcggggtg ttcgctgagc gttctcggtc tcaccgcttc ccggagcaaa
     5401 acgcactttg gatcctgagt acggcaaagg ataccgcgaa ctttacgagc ggcactggtg
     5461 gtggcgctcc cgagaagtag ccgttctctc cgtgcttcat cgccatcggg ggtcggcatc
     5521 ccccgcgcgc atcctcgaca tcggttgtgg cgacggcctc tttttccgtg ctctcgagga
     5581 gttcggtgag gtggaagggc tcgaatcctc agcggccctt atcagcccgc acgggccgta
     5641 tcgcgcgcgg atccacgtag ctccattcga tgcgcgcttc cgtagcagca aggagtatga
     5701 tctgattctg atgctcgacg tcctcgagca tctcgacaac cccgctgaag cgctgcggct
     5761 cgccgcttca ttgcttccac cgggcggctc gctgctcctc accgtcccgg ctttccgtct
     5821 tctctggaca aatcacgacc tgatcaatca ccaccgtaca cgctaccggc gagggacgtt
     5881 gcggccgctc ctgaaagaag caggattcct tgtggtcgac cagcgctact ggtatcagtg
     5941 gacttgtccg gtcaagctcg cacagcgctt cgtcgagggg ttcttccgac tcccgccggc
     6001 cctccccact gtcccgccac actggttaaa cactctgctg taccgattct ctcgattcga
     6061 acaggagacg ctcggcgcgt gggggattcc ctttgggagc tcgctgatga tgtattgccg
     6121 caaggctaat cggtgaagat tgttcgctgc atccgatgcc atcgttgtga aacggcaggc
     6181 taccgctcgc tcgcaagttc cggtgcggca gaacgctgat tcctagcagc ggactgctgc
     6241 gcgttgtcaa cgaagcccgc ctgactggct tcacggtttg cggaggagaa cgggaggcac
     6301 ttccccgggt acgtaaggcc gcatctgttc ggaaacgctt tcaggctcga tcagccagac
     6361 tttccggtcg tggtagtagt tgaggagttt caggttttgc tccgggtcca tctcgcgcgc
     6421 ccataccacc ttggatccat ctatatccgc actgttgtaa acccattctt cgccaacctc
     6481 gtggttcaag ccgtaccgca cgatggcgag ctgcttgccc ggcttctctt ccaattgttt
     6541 cccaatttct gcgcgctccc agttgcccat gtcgccgttc cagccccagt ccggatgccg
     6601 gattcggtcg tagaggcatg tcgcggtcgt gccgaggagg aaaagaaaga tcatacgcga
     6661 cagccccagg ccaacccgct ggcccgcgga ccgaaaggtt cgaaggtgtc gcaggctctg
     6721 aataagcaac gcatacaaca cgcacacgac cggagccgca taatgcggca gggaccaaat
     6781 caccaggaag agtcccagcc cggccagcac aaatgtcagg atcagaaatc tcattcgccg
     6841 gtcgcgaaac acgaaaggcg cgaagatgag caacggaatg gcgcccgtcc acaggaaacc
     6901 gtcatcataa gcctcaagct tcatctccga gacgcgcttc accccttccc aggagcgatc
     6961 gtagcttgag cgcaccacac cattgtagaa gtccatcatc tccaagtttc ggtattggcg
     7021 cggcgggcct ggtttctccc agaggaagag acctgagggt atgtagttcc gcgaattcaa
     7081 aacgtgtggc agcaagaacg cattgcccgt cagacgccag tcgtagtaca gagtgaacgc
     7141 tccggtcagc accaggatcg ccaaaaccgg cgcgaccacc cggcgcattt tcaccttccg
     7201 aggcaccttc gaattctccc atagccatac caggagcact cccgctgcgg ggacgcagaa
     7261 gatcaatccc tcgagtggcc ggctgttcgc aaggattgcc acgcctagcc cgagcagcag
     7321 ggcgtcttgg agtcgcggct tccggagaat ccgcggaatg gcgcctgcca ccaatgcccc
     7381 gccggcggcc gctaccgctc cgccccagta actgttcatc cagtagctgg tgactcccag
     7441 ccggaggaga gtaacgattc cgccaaggag cgcccagcgc ggcggcatcc atccctgaag
     7501 catccagaca atcgccgcca ccatcgccgc gcagctcagc agcacgccga tccatggatg
     7561 ccccgcaagt tgtcccaggg ccagcacaaa actctgggcc ggggggaaca tgctggaata
     7621 ggtcggaaac cagttgatgt gaaacgtttc tagactcacc cacatgggat ggggagggtt
     7681 ggcaagacgc cagtgcgcga acgtatccgc ttgtaacaag tacgaaaact cgtcgtggac
     7741 tcccggcacg ggcaccggga tgagcggcag aagaagaagg cgaaggccga tcgtcgcccc
     7801 aaaaatcatc aagatggctg ccgatcgtct cgcggcaatc cgcgaaaaaa catcctcaat
     7861 cgagcggaac catcgcccac ccaactccgg catgagcaag gcgatcgcca ggcaaatggc
     7921 cactaccagc cagttcatga acccttcctg ctccagtttg acacggccgg gcgtgccagc
     7981 aatagccgcg attctcaaac tcaggcgggc gacttcgttg ggtccacgcc gaaacgggca
     8041 gcggtttcct tccacatacc gtcttttcgc gtaagtggct ttcacttctt gccgccagcc
     8101 ctcgtcccgc gctggcctcc tggccggctg ttttcgatcc cttctgcctc cttcgctcct
     8161 tggctcgaaa aaaaacgggc cgcggcgctg tgctgcggcc ccttgtttga gtgacgattg
     8221 gatcgatttc tatttatgct ctggctgatc tatggtttcg aagcgctttc gatgaagctc
     8281 cccaagttct ttctcagttt cgctcggtct tcatcccgga tatacatcat atgtcccgcc
     8341 tcgtagtacc gcatgtggtg gatgtggcgc acaaccacga attatacgca ctctctgtag
     8401 acgcaaatcc tgctctctcg gtccaacttt ttccgtctca ctctaagctt ttgccaagct
     8461 gcggcgtgcg cttctcgtgt cgaggttgcc gcaagtcgga cccatcgaag ggccaccccg
     8521 tcggggatga gtgcaacgtt tgaggttggg gtgggcgatg ggatttgaac ccacggcgtt
     8581 cggagccaca gtccgatgct ctaccagctg agctacgccc accacctttt ctcatggtaa
     8641 cacggagcct ctccgccgga gaacgcccgc tcgccgtgat cccttggtag cagcggctgc
     8701 ctccgaaatg acagtagcag cctggcaacg ccccattgcg cgacctcacc ggcttgctag
     8761 actcaatccg agtgtaattg cgtccccgcg catggaattc cagacctccc atcgccgcat
     8821 ctttgagcga caactgacct tcgcgcgccc catcatcatt ttgctcgccc tcctcgccct
     8881 tctcgatcgt cccctgtcgg tcacctcggg gcgcgcattt tattttcttc tgggatatct
     8941 cctgttctct gtggcgctcg tcgcctcaca tcgcctcttc gaacgccgcg atctgtttct
     9001 tcccctggtc gtagacctcg ccgcgctcct cttcttcctc tacgtcagcc cctctctgac
     9061 cgccgtttgg ttccccttcc tctatgtcgc gttcgccgcg ggcggccgct ggggcagccg
     9121 ggctgccgtc ttcctccttg gagcgctctc cctggctctc gttttccgcg cggtcatcca
     9181 gggtgaatta cacctgatgc acatcatcgg atggctcggc ctgctcgggg ccaccttcgc
     9241 cgcgggcgtc gccctcgccc tcctcggcga tctcagccgc cgcgccgccg ccgaaaacga
     9301 cttcctgacc cgcatcacca ccactctcca ggtggatcag ggccttgcgg agtctttccg
     9361 tctcttcctc gacgagctgg cacgggcctt cgacgctgcg cagtccttga tcgtctttcg
     9421 agacctggaa ctcgagcgca ttttcctctg gcgtgtcgtc gtcggccaga ccgaacgcat
     9481 ctccccggag aatctcccgc tcgcccgcgc cgatggcttt ctcctcgacg acatggacgc
     9541 cgacgtctgc tggaacagcc tcgaaggccc cggccaggga ttcggctggg accgtcggaa
     9601 cggccgcagg atcaaattcc ttcctcatct gcccggcccg tcgcagcacg aattgcaggt
     9661 ccgctccctc atgacggtcg ccttcgacca gtccgggcag cccgccggcc gccttttcgt
     9721 cgtcaacccc cgcaggccct gcacccgccg cgatctccgc tggctggatc gcatcgcccg
     9781 ccatttttcc gcccccgccg aaaatctctt cctgctccgc cacatgcgtg cgcgcgccat
     9841 cgaaggcgag cgcagccgca tctcccgcga cctccacgac ggcatcctgc aaaccctcct
     9901 cagtatcgag atacagctcg acgtgctccg ccgccggcta cccgtcgcac cggaccaggc
     9961 cgttgccggc cttgccgcgc tgcaacagac tgtcaagaac gaatccgcag agttgcgcgc
    10021 cctggtcacc gacctccgcc cgcttcgcgt tcagagcgcc gacctcgtgg acttgatgcg
    10081 cggtcacgct gaacgcctcc gcaacgaatc cttccttgcc ctcgaactcc ttattgactc
    10141 cacggaaatt gaagctcccg accacgtttg ccgagagctt tttcagattt accgcgaagc
    10201 gctgagtaac gtaaaaaagc atgcgaaggc ctcccatgtc gtggtaaaac tgacgcagga
    10261 cgacagccgc atcatcctcg ttgtggacga cgacggcgaa ggtttcagct tcgccgggca
    10321 gttttcgggg gatgagcttg atcgcctgcg gctgggcccc atctccatca aggagcgaac
    10381 gcgcgcggtg ggtgggatgt tgaccgtgga gtccagcccc ggtcatggtg cacgagtgac
    10441 ggtcgaaatc ccgctagctt gatatggcca agaacaaaca gcagatccgt gtcctcatgg
    10501 cggacgatca cgccattttc cgcgatggcc ttcgcaagct cctcgacgcc gacgatgacc
    10561 ttaccgtcgt gggtgaagcc cagaacggcg ccgagtgtat ccgcatgctc ggaaagctca
    10621 agccggacat cctccttctc gacctccgca tgcctgataa agacggcctc gccgtccttg
    10681 aggaagtcaa cttcgactcc cttccgacgc gtgtcatcgt tctcacggcc gccgaagacg
    10741 atcgcgacgt tgtccgcgcc atgcgcctcg gcgcacgcgg cgtcgtcctc aagcaatccg
    10801 ccaccgacct gctcgtcaag agcatccacc gcgtccacgc cggcgaaatc tggctcgaca
    10861 accgcatgac cgccgaggtc atgaaagcct tcgccaaatc ctccgaggct ggcccccgcc
    10921 gcgaaaagcc gctcctcagc gaccgtgaaa aggaaatcgt tcagctcgtc gctcagggct
    10981 ttcgcaacaa ggagatcggc gaaaagcttt tcattagcga acagaccgtc aaaaaccacc
    11041 tccacaatat cttcgacaag ctcggcgtct ccgaccgcct cgaactggcc ctttacgcca
    11101 tccaccaccg gctcatcgat catgcttagg tctcatttgc cggctcctct ctcctgtttt
    11161 cttcaacctt aggagcttcg gtctccttca aatgctgttc cacctccttg cagatgaacg
    11221 gagccgactc tccgtctggc ccgagagtcg tttggttcga cgagcgcgta ggtacgtcaa
    11281 gttggacaag cactagtacc acagtactgc agcgtagcct tcctcctttg aacctcgagt
    11341 cctattgtcg aggggtacca tccccgttag aatctctaat tcagacagga ggcccatcct
    11401 tggcaggttc tgaacgccgc acgacccagc gttttcagat gcggttgcct ctcaccgttc
    11461 ggtggaccac gggcgccgcc atcggcgaga caagcaccga gtcccgcgat gttagctccc
    11521 gtggtgtcta tttctctctt tcgaaagatg ttaaggaagg ctcgccggtt gaaatccttc
    11581 tgaccctgcc gaacgagatt acattggccg gcccggtgaa ggttcgctgc ctgggccgca
    11641 tccaccgcac cgaagtgcgc gacgaagcca ccgtgggtgt ggtcgcggtg atcgagcgct
    11701 atgagttcct gcgcagcgag gaagaagagg caaattgacg gatttcttgc ccgcatcctg
    11761 cggaggcgga caggataagc cggggtgcct cggcccctca gcagagcgcc ccggctcttc
    11821 ttatttcttg agcagcgttt ccagccgcgc gaactgttcg ctccaataca ccttcatctc
    11881 ctgcacggcc cttgcgctct tcagatcgcg gtgatcgatg gtcacttggc tatttcccgg
    11941 acccttcgga tagaacagga tttccacccg cgatttccct ccgtcccaca ttccgcgaag
    12001 cgctttgttc ggcctcgccg ttgagacctt cgcgaggcca cgcactggaa gacagtacaa
    12061 aagagaatga ggagtttgtt tcgcaaaaga ctggagtcgg tgcactttac ctcgaaaatc
    12121 ttacagggca ctgctatgat cagccccaat tctccgggga tgccgaaacg atcattgccg
    12181 aggacaggct aagacctact tcttatgggc aagatgcgac ttttcgagat ccacgatcag
    12241 gcttggtttc ccggttttct ccgtgatcaa acaacggatg cccttcaatt catcctgaat
    12301 actgggaacc tttatgggcc gatcgttcac cagctcagcg gtgctttggc agagtccggc
    12361 acagtccgga ttcttgatct gtgctcgggt gcgggaggac cctggctaac actttcgaaa
    12421 gctcttgagc aagaaagggg tctgccgtta catatttgtc ttactgacaa atatccgaac
    12481 gtcaaaactt ctaacactgc gaaatcgcta gcggggaatg gggttcggtt tcactccatt
    12541 ccagtggatg ccacgcaaat cccggacgag ctgaacggtt ttcgcaccat tttcagctca
    12601 tttcaccact tcagaccgtg ggaagctcga gcgattcttc aggatgccgt caataagcaa
    12661 cggggcatcg gcatttttga agtggctgga cgaagtgctg tgactgttct tctcaccttt
    12721 ttgatgccga tcgggagcct gctactgact ccgttcatgc ggcctttccg ttggtcgcgt
    12781 ttcctatgga cctatatcct tcctgtaatt cctttcgtgc tttggttcga tggaatcatg
    12841 tcgtgcctgc gtgcctattc tctcaaggaa ctcattgagc tgacgtccgg gctcaccgta
    12901 catggatacg aatggaagat cggacaagaa aggaatggtc tcctccccat tcccatcacc
    12961 tatttgatcg gctaccccat tcgcgagact cgggaatctt gggatgttcg agcctgatga
    13021 aatgcgggga tgcgcgcaag aggccctgaa catcttttcg cgaggctttc ggaccaagcg
    13081 gatggtgcgg gccacgccta tggccgtgaa tattgtcgta ggccgggtcc cggcatcgca
    13141 ttctaggttg ccgtccaagc gccttgactg tcgcttattt atgctagact tccgccccga
    13201 aagattgcgc gacgattccg ggcaagacgg cagtgacaat ttcgttccga taacgcgcgg
    13261 cgttgagtga acaataagaa caggactctg ccaataaatc gccctggtct tatatcccct
    13321 attcttgaac gttcgggcct ttttgtttcc taggagggtg gcacaatgaa aagactgaac
    13381 aagttctgta tcttgtccgg gctgcttgtg gctttgacag ggaccctttc ggcgtacgca
    13441 caggttgatt atgcgacagc tacgctccga ggaaccgtac tggacccgca aggagcaatc
    13501 atctcgggag caagcatcac cgtgaccaat tcgagtaagg gaatcagcag gacagtcaag
    13561 accgccggtg acggcacata tcaggtcccg gcgcttccgc cgggggccta tcagatcagc
    13621 gtcgaggcca atggcttttc gaaagaggtc gtcaaggccc tggaactgac cgtcggccag
    13681 agcgcaattt acgactttca tttgaaggtg ggcaacgcca gcgaaatcgt tgaagtcaca
    13741 gcagacaacg cggccctgat tcaaaccgac cagacccagc aagctaacac tatcaactcg
    13801 ctgcaggtcg aagagttgcc caacatcaac cggagcttca cctatgatgt gtacaccgtt
    13861 cccggagtga gcaactcgga agccccgcgc agccagaacc cgggatttac gggatacctg
    13921 accacaggct tttccatcgg cggcagcaac ggccgcaaca atctctccac cattgacggt
    13981 ggtgagaatg agtatggcac cggccagtac agggtcacgg cccttcccgt ggatgccatc
    14041 caggagtacc aggtcaaccg caacgccttt gccgccgagt tcggatttac ggacggcagc
    14101 gccatcaaca ttgtcaccaa gagcggaggg aaccagtggc acggtgacgc cttcggccat
    14161 ttccgcgacc agcgcaccga ggcgacaaat ttcttcaatg gaggtcccac caaggcattc
    14221 agccaaaacg tctatctcgg cggctcgctc ggcggaccca tcgttaggga caagctcttt
    14281 ttctttaccg cctacgaatt tcagaggctg gatacgccga atttcggcaa cgccggcctc
    14341 ctcacccagc cgacggtgct gggccttaat gcccccgggt tgggggccaa ctgcgccggg
    14401 cagtttgcca gcaagactcc ggaccaactc tgttacatca atgcgctcaa gagttctgga
    14461 aatccttttt tggtgggctt cgccaacggc atcacgccgg gattgagccc gctgaataat
    14521 ccggcgctga acaccatcct cacccgcgac aatggggttt tcgatagccc cgacaggttg
    14581 cacaacgcca tcatgcgctt cgattaccag gcgagcggca aggattccct cagtatgcgc
    14641 gccggctacg tacacaacaa tttcacttcg ccgctgatcg gtattacgaa cggcactcct
    14701 gacagttaca gcctgttcgt cagggatttc agtattctta ccacctggac ccgcagcatc
    14761 agcacgaact gggtcaatca ggtcctggtt cagattgttc cgcgcaacag ctcggaagcg
    14821 attccgctcc acaacaatgg gattaacttc agcttgggca atctaggtgc tcccgggccg
    14881 ggcggcacct ccacttttgg gggccccgca ctccttccct ataaggccca ccagcgacgc
    14941 taccagttcg aagataacgt gacttggacc agaggcgccc atacgttcaa attcggcgct
    15001 tcctaccggc ccgccgacta ccatgtggaa aatgatctgt ggttcaaccc cgagtttgac
    15061 ttccgggacg gcatcattcc gctcatatcg ctggcgccac cggcggtcca agcacaactg
    15121 gtgggcttta atcttgccca cggactgccc gcaaccggcc cggtaaccac caacctgact
    15181 gctcctcagt cttttgcgtt cgggatacca gcagacgtga tagcgggatt caacaacccg
    15241 aagtgggaag gatgggggca ctatttcggc agctacgtcc aggatagttg gaagctgaac
    15301 tcgcggctga cgatgaatgc cggcgtgcgt ctggactttg atggtgagcc tccgccgctt
    15361 ggcggcagct tctatgccgc tccccgcgtg gggttcgcct gggacccgtt tggcgaccac
    15421 aagaccgttt ttcgtgccgg ggccggcatt tatgttgcgc ccgttgatgt cctgattccc
    15481 tcgtatgggt ccttgctgga cggcaccggg cgatacatca atcaagtgct tgtccctctg
    15541 agtccaacag atccacgggt agcccagctg tggggtctgg gcctagcgaa aggtgagctg
    15601 ccgttcgggc aactgacccc cgcggactac gctgcggttg gcattgacat tacgtcgccg
    15661 ggggctacgg ttggttacag cgtcgcaccg aattacaaga atccctacag cctccaggcc
    15721 agcctcagca ttgcccaaca actggccaag aacctgtctc tggaattggg atacaacatg
    15781 taccacggcg ttcacctgca gacgccggtg gaaactgcct atgctgagat tccggttggg
    15841 tctccgttct gtcccactcc ggcatgcacg gatgcaaccg gtggtccgct atatcggccg
    15901 cttaccggcc agttccagca cacgacgtac gcttccaatg gcagttcgat ctaccatggg
    15961 tttacggctt cgctgaccag gcgtttcagc cacggcctgc aattccaggc caactacacc
    16021 tggagcaaga cgatcgacga caccattgac ttttccagct tccagaactc gttcaggccc
    16081 agccgcctga acctgtttcg ctccatatcg gtttttgatg taccacacat cttcgtcgcc
    16141 aatgcggtgt acaggctgcc gttcaaagct ggcgagggca agccactgtc cagcattctt
    16201 gctgacataa ctcttgctcc catcttgacg atgcacagcg ggctcccctt ttcggtgagg
    16261 acgccaagcc tggtaaacaa gatcaatggc caggctctgg acaataacta cgccacgccg
    16321 ttccttgccc cgcgcgatgg caatcgagga gccgccttct attccttcga cctgagcttt
    16381 cagaaggccc tgtacattgc gagagatcgc ggagtgcggc tgaacctgat tgtgcaggga
    16441 acgaacctgc tgaaccacgt gaacttcaat cacgtgaatg acgtgttcga tctcaacggt
    16501 atcccggcca gcgggatcgt acaaacggga actggtcctg tgaacctgct cactgggccg
    16561 tttacgggtc tgcgcgctgc cgtgcccacg aatgccggcc agctcacaca gccgctgttt
    16621 tattcgcagg ctgatgtgcc gcgccagatt cagttcgggc tgaggttggc gttctagccc
    16681 atctcaattc gtgagatggt ttgcggcaaa ctagctattg ctatgccgag gccactaggt
    16741 acgcagaatc cccgctggcc ttcctcggct gcgtgaccaa ggctcattgt cactcggccg
    16801 gcttgttctc gattaactcc gctaagttca ccaggatcat gttttttcct cgggtccgaa
    16861 gattgagcca aagaaaacgg atcggcggcg gcgtatcatt tcgtctacgg cgtgctgcat
    16921 cttcgttcgt acttccagac taattgcctt gatcacggaa gcatcctgag cggcttccgg
    16981 ccggtattgt tcttccaggt gatggatcgc agaagggaac tggtcggggc gcgcggattt
    17041 gaaccgcgga cctcctgcgc ccaaggcagg cgcgctacca ggctgcgcta cgccccgaca
    17101 agtgaatctt agcatagcga gtcgcgccag cagaccgcga gcccggtagg ggcccggcgg
    17161 gtgtgaaggc ccccgccgcg cccttgcggg gggcggggcc gcgccgcttg ccctctgcgg
    17221 gcatttattt ggaaacggca ctggacaaga atcgcgttcg atggcaggct tactgccaaa
    17281 tgattgcctg ttgcaggcca tttctgacga aagcaggggc gtataaatga gaaaacttct
    17341 tggaagcatg ctcgcggttc tattcctggt tgcggggacg gcgcgcgggc agggcgttgg
    17401 cgcctccggt gatatccggg gaaccgtcac cgatcctacc ggcgcggttg tgtccaaagc
    17461 gacggtcacg gtcaccgacg ccgagaaagg catcaagcgc tccgccgtga ccgacccgga
    17521 cggcgaatac cggttcacag cgctgccgcc caccacctac gacatcagcg tgcagttcag
    17581 cggctttcag acctacatcc agaagggcgt ggtcgtcaac gtcggccaga ccgtgacctt
    17641 ggacttccat ctcacagtct ccagggcggc cgaatcggtg gaagtcacca ctgaagcgcc
    17701 cgtggtggat acgcagcaga gcgcccaggc gaacgtgatt gacacgcaga ccatccaaga
    17761 cctgcccatc aatcgccgcg actacctgac gttttcactg ctggcgcccg ccgtcaccga
    17821 ttccacccgc ttggcgggtg accaggattt tcgcgtcaaa cagacgcccc agagcgggct
    17881 ttctttctac ggaagcaacg gccgcggcaa cagcgtgacc gtggacggcg gcgaagtcaa
    17941 cgatgacgcc ggcggcgtgc gtctcaccct cagctcggaa gccgtgcagg agttccagat
    18001 caatcgcagc aactatggcc ccgatctggg cggggccagc ggcgcttcca tcaacatcgt
    18061 gtccaaatcg ggcagcaatc aggttcatgg caccgttttc ggcctcttcc gcaacgatgc
    18121 tttcgacgag cgcaacccgt ttgctttcac ccagcctctg gcgccgggcg cgaccttcaa
    18181 ccccgcactt cccgatgtcg ccggacagcc gacccaggac tccctgagcc gttatcaatt
    18241 cggcggctcc gtcggcttcc ccatttccag ggacaagact ttcgcattcg tggccgctga
    18301 aggcctgatt tcgaatgcgc agaatgccgt gccgttgttc accaatacca atgtgtttcg
    18361 gccgaccgcg gcccagcagc cgatcattac gggactggcg accctggcag gtaatcccgc
    18421 cgtgccgtgc cttaccggac agccggcact cccggcagca acttgtgcgg tcatcctgac
    18481 cagcatcttc actattaatc cggccgcggc caacccgggc accagagcgc gcaaccaatt
    18541 ccttatcaac cagttcgaag ccaacggcgg attgttcgcg tatgacaccc gcgagtactt
    18601 catttccggc cgtctcgatc accagttcag cgaacgcaac caggcctatc tgcgttacag
    18661 cttcgcccat gacaatgaac agagcccgga cgtgcaatcg ttggtaggat tttcagccgg
    18721 cagttccatc gcccccgcat atgacaacac gctccagatc gcatggttcc accagttcag
    18781 ccccaggacg ctcaacgaag cccgcgtaca gtggaactac tcgcatttcg acgtcattcc
    18841 taacgccccg ggcacggtcg gcctgaacat gcccgggttc gggttcctga actccaacat
    18901 cttcctgccc agcaagacca tcatgcgccg atacgagttt gccgacaatt tcagcatggt
    18961 tcgcggacat cacagcctga aatttggcgg ctatgagctg atccgtggca accacaccga
    19021 gtctgacacc ttcttccccg ggcgcttcca gtttggttcg ctgcccggtg gtatcgtaac
    19081 cccctgcttt caggtccccg cggcttgcgg tctgaccggg gtcaacgccg ccaccatcag
    19141 ctcgctccag tccttctccc tgggcctgcc ccagttctac cagcagggtt ttggcagcgc
    19201 catctacgcc attaaccggc cctggaccgc tttcttcgcc tccgactcat ggtccctgag
    19261 gtccaacttc acctttactt tcggcgtgcg ctatgaattg gactcccagt acggcagact
    19321 gaagaccgac accaacaact tcgcaccgcg cattgcgttt gcctgggacc ccttcagcga
    19381 ccacaagatg gcggttcgcg gcggttacgg catcttctac tcgtccatct atgggcagat
    19441 tcccaacgtg atccagactc tgggcctggt gaatgggttc caacaaatcg cccaggtctt
    19501 tgtccccctg accggcgagc ccggaaaccc ggcgctcacc tcggcagcca tcttccagac
    19561 gctgtttgcc cagggcaaag tgcagtgcac ggctcctgcc gccgggaacc cggcctgcat
    19621 tacacccgcg gacctggccc agttcggcat caccatcaag catgacggta cggctcctct
    19681 gagcgtcttc ttcagcggcc agccgaatta tcagaaccct tattcacagc aggccgagct
    19741 cagcgttgag cggcagatcg gcaagagctt ttcggtttcc gtgagcggtg tttacgtgca
    19801 caccatcggg ctgccgactt cggttgacgg gaacgcgctc ccagcgccga ttacaaacgt
    19861 acctttggca aacggttcga tcgtttcagt ccggaactgg aattcgcgcc ttccaaatcc
    19921 cctgaattct gcaccgtgtg caggagcgac catcgtcaat tgtttcgtga gcccaaccac
    19981 gctgcaaaat aacttctatt cctccagggg tgcggccatt tatgaaggtg gcattctgga
    20041 aatcaagaag cgcttcagcg accacatgtc gctgatgttc aactatacct tcagcaaggc
    20101 cttcagttct tccaccgact tcaacagcga cttcggaccc caggacaaca ccaacctggt
    20161 cctggaacgt ggcctctctg acttcgatca gcgccacaag ctggtcattg ccgccgtgct
    20221 ggaaagcccc tggaaaaacc gcttcttctc cggcttccag gtcgcaccga ttgttcgcta
    20281 caacagcggg cacccgttta acctgctggc cggcacggac gtcaacggcg accggcactc
    20341 caccaacgat cgccccatcg gcgttgctca caacaccggc gtgggccccg actttgctac
    20401 cttcgatatg cgcgtaacca agcgcttcaa gttcactgaa aagacgatct tgagcatcat
    20461 cgctgaagga ttcaacatct ttaaccagat caactatgcc agcgtgaaca acgtggttag
    20521 tccgaacctg ctgctgccca ctgctgcggg cgggttgggc ttcaccaccg ccaacctgca
    20581 cggcagctca gccatcgcac ccaaccagcc gctgggcttt acctcggcct tccccacgcg
    20641 gcaactgcaa ctgggcgcca agctgacctt ctaagagtcg cggggcctgc gcgagccggc
    20701 ccccgcgcca cgctgccgcg tcgaggcgcg catccgagcc aaaaagcaaa gcgtctgaac
    20761 aattcagccg gaaatggatg cccatccctc ccatcgggat gggcatcctg cttcttagcg
    20821 gccagatgag cggataacgc cgcgcttctc actcgtaacg tagtgcctcg acaggattca
    20881 gccgcgcggc ccgcgacgcc gggaagattc ccgccgcaag actcactgca acggagaaag
    20941 caatcgctcc gccaaccatc caccacggca ccgaggcgag ctgaaccgaa gggagatcct
    21001 gccggtgaag atagatattg gctccaaagg ttaggccgcg gccgatcagc ca
//
LOCUS       DASYVO010000338        11195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_206148, whole genome shotgun sequence.
ACCESSION   DASYVO010000338 DASYVO010000000
VERSION     DASYVO010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 11195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11195
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206148"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..2529
                     /locus_tag="VGR03_11470"
     CDS             232..2529
                     /locus_tag="VGR03_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEV1994941.1"
                     /translation="MSRSLVALILIVGLSLGGAFDAFAQSSKHQRKTKKPKSPPCRTG
                     CKPDTSAPQVAADTPEDEAAQKELSDLARALHNATPAAYEKLSAFAVKNTTNVWGARA
                     ALALGYDDYSKNRTAQGLGWLIKAQNDMLLREYALYWTAQAQRALGRSADAYKVLQSI
                     QHDYPNTAMREQLLEAFAPTAVQLGHAQEAIDALDAYSATASKPTLLYERAHAYQAAH
                     QLPRAAKDYQTVFYKFPLADEAKPAGSALSQLMHALGKEYAYPGVELQEQRAQAFYDV
                     HKWKEARAEFEKLLTMLKDVANPARQRAQLRVAECRVQLKGSPSLIAAVKTPDLEVDA
                     ERLYALSQAYRSAKKEAEMFSALNTLAQNYPVSKWNEEGLMAAANYHWVELERTKAVI
                     NYQRVLDTYPNGKYAFNCEWRIAWVAYLNRQPDADDRLTTFLLKYPVSSNSVDALYWL
                     GRNAERGGNPAHARSYYNKAAERFPQTYFGNAAAARLAKLGPGEENPAELLEKIPPPP
                     SLRPFDEPIPVAAADRWARAQALRAIAFDASAEQELKNAYFATSSPRFLLEAAQAAFD
                     QGHYGAGMAYARIIVPNFDSRKISDVPVAAWKALFPLPYEAALRREAAKNDFDPMFAA
                     GLIRQESTFQADAVSHANAIGLMQVLPKTGKLLAKQLKVRYTKDKLFEPDYNIELGMV
                     YIASLVRQTGALEYAAAAYNAGEDRIAAWKAERNYDEIPELVESIPFTETREYVQVVL
                     RNTEVYRMIYGQPSVATQVAIGHAR"
     gene            2619..3839
                     /locus_tag="VGR03_11475"
     CDS             2619..3839
                     /locus_tag="VGR03_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV1994942.1"
                     /translation="MTAPDLSMAQISRRMLSAKAEQFTESVIREMTRLALKHGAVNLS
                     QGFPDFAAPEEIKEAARKAIADDINQYAITWGAKPLRDAIVEKFERTQGVKYDPEREV
                     TICCGSTEAMMSAMMAIINPGDEIVVFEPFYENYGPDAILSGATPRFVKLRTPDWSFD
                     EKELAAAFGPHTKAIILNTPNNPTGKVFTRAELEFIRDLCVRWNAYCITDEIYEHILY
                     DGAEHISMARIDGMRDRTIVINGLSKTYSVTGWRVGWVLAPPEPTQPIRKVHDFLTVG
                     AAAPLQQAGASALHFPQGYYDKLATSYEVKRERLLKILTSAGFTVFKPRGAYYIMTDI
                     ARFADPDPIRYQADTKDVRFAKFLVETIGVAVVPGSSFYNNAQDGASQVRFTFCKKEE
                     TLAAAEERLSKLRP"
     gene            3890..4852
                     /locus_tag="VGR03_11480"
     CDS             3890..4852
                     /locus_tag="VGR03_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994943.1"
                     /translation="MRTFFTAIVFLSVTSLLAQDTRPSRAFDYDAKAPLDIQEAGVEH
                     RENVVIHNISYASPKGGRVPAYLVVPEGKRPFAAILWGHWYWQNSAFFNRKEFLDEAV
                     ALAHTGVVSLLPTGPGARAGHVQDKDPLNDQQITELVQAIVDMRRGADLLLARKDVDP
                     QRLAYVGHSYNAVVGGFLSGIDKRFKSFVLMAGGLSDEVDMKTEDFQQFRQKIGPEKV
                     DAFTAKYAWTDPGKFVSHATPATVFLQYAAQEKFLTAERARQYAAIVSEPKQFKLYDA
                     PHALNAEARRDRIAFLSDQLKLKPLDPSLVTGIPDLPQPPEPAP"
     gene            4962..5375
                     /locus_tag="VGR03_11485"
     CDS             4962..5375
                     /locus_tag="VGR03_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994944.1"
                     /translation="MPTELDLIPHDTRMEANGDGAAFDISASATRTFFCRLTVTDQIE
                     QESLDVSIWGSADAETWTKKPLLKLPQQFYRGTTKMILDLSLRPEIKFIRARWEVNRW
                     GRVAPTPMFVAGLELAEIPPMSRETPTTRIALAAK"
     gene            complement(5631..7034)
                     /locus_tag="VGR03_11490"
     CDS             complement(5631..7034)
                     /locus_tag="VGR03_11490"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1994945.1"
                     /translation="MAKLTFLGAAGCVTGSKYLVEAAGKQLLVDCGIFQGTNELKDRN
                     FKPLPVDPKTIDYLVLTHAHLDHTGWLPVLVKSGYRGPIFANPATIELTTILLKDSAH
                     LQEEDAEHARLKKYSRHPDPQPLYTTEDVDPTLKLMKSMPRAGGFDVSPEFHVASYDA
                     GHILGSSSLELTITESGRKTVVVFSGDIGRYNQAILKDPTTPPSKADVLICESTYGDR
                     EHPEGDPAELLAQIVNRVVKRGGSIVIPSFAIGRTQTFMYYLRQLEDQQRIPRLPVYV
                     DSPMALSATDLYLKHKEDHDLEYVRQEGSDGKGDPLNVHEFHLTRSAEESKAINNVKK
                     PCIIISASGMVSGGRVLHHLVQRLPDARNAVILAGFQAEGTRGRALQEGAKSLNLYGQ
                     VVPVCAEIVEMGQFSAHAGKSELLRWLTALPAPPKQTYLTHGEPAAAQALQAAISEKF
                     QWKAAVARYLDTVPVGE"
     gene            complement(7183..8676)
                     /locus_tag="VGR03_11495"
     CDS             complement(7183..8676)
                     /locus_tag="VGR03_11495"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEV1994946.1"
                     /translation="MPVDKKKIMTTDAGRPVGDNQNSLTVGPRGPVVFEDFLLFEKMA
                     YFNRERIPERVVHAKGSGAYGHFVCTSAEMTKYTTARLFGALGKKTPTFLRFSTVGGE
                     KGSADTARDPRGFALKFYTEEGNWDMTGNNTPVFFIRDPLKFGDFIHTQKRDPETNLK
                     SPTMMWDFWSLSPESLHQVTILFSDRGTPDGYRHMNGYSSHTFSLITAKNELFYVKWH
                     FKTKQGIKNFFREQAGALEGTDPDYSQRDLFHAIKAGNFPKWRVCVQIMPEKDAETYH
                     INPFDLTKVWPHKDYPLVEVGELELNRNPENYFAEVEQAAFEPRNVVPGMGFSPDKML
                     QARLISYPDAHRYRLGVNYDTLPVNKPQCPFHTYNKDGAMRFDENGGASVNYEPNSFG
                     GPTQDRSYVEWPHAVSGTVARHDHRTDSDYYTQPGNLFRLMPADAKQRLIDNIVSSLG
                     NGVPKRIQELQIQHFYKADPAYGTGAAKGLGLKIESIVAKKQASAAD"
     gene            8803..9684
                     /locus_tag="VGR03_11500"
     CDS             8803..9684
                     /locus_tag="VGR03_11500"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1994947.1"
                     /translation="MELHQLRYFCAVAETGSFSRAAEQSHISQPSLSQQILKLEDELS
                     ARLFDRLGRSVRLTELGKTFLPRARAVLRELEAAKGDVVEGKEFIGGPITVGVIPTVA
                     PYFLPPRLTTFSRKFPQVQLTVVEEITPVLLDRLRAGTIDVAILALPIRGHEFDAVPL
                     LTERLFAALPKKHKLSSRTSLSLKDLRAEPFLLLRDGHCFRDTAVAACDRARLYPQIV
                     FESGQFSSLLSMVGAGMGVSIVPEMAIEKKSQCRYIRIADDQAVRTIGVVVLRGRSLT
                     RAHQSFLSLVNSSKASS"
     gene            9785..10918
                     /locus_tag="VGR03_11505"
     CDS             9785..10918
                     /locus_tag="VGR03_11505"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEV1994948.1"
                     /translation="MTAQTPPLLEAPIPPKTAAAPPKSSKAKKKLIRRTDRDYSQLVR
                     RSYQAAFLLWNVWLGGIFYFWVCGHEAGTATVDVAPPPGIEGWLPIAGLMNLKYFLLT
                     GLVPSIHPAAMFLLVTFLAMSFLFRKAFCSWLCPVGTLSEYLGLLGKKIFGRNFRVWR
                     WIDIPLRGLKYFLLGFFVWAISSMSAAAIANFMRSDYGLVAGVKMLNFFRHLGETGLI
                     VVGILVLASVFVQNFWCRYLCSYGALLGFASLLSLVRIRREPEPCIDCGKCAKVCPSS
                     LPVDRLVTIKSAECTGCLECVAICPAEGALHLALPRFRNPPRRIPSWVMAAGIAVLFF
                     GVVGFAKAVGLWESHVPGAVYRQLVPHASQARHPMPGDPEISR"
BASE COUNT         2240 a         3466 c         2981 g         2508 t
ORIGIN      
        1 gattgaaaga cgacattgaa aaagctcatc gagaatacga ccgccgcttc aaaccgatta
       61 tggatcaccc cgtcgattac ttttatcgct ggatggtgga aatccttgcc gaaggggacg
      121 cgcacgccct gggcgaatat ccgtacgcca cgcccgcgcg ccatcactaa tcgaaggctg
      181 gctgctcgct tttgtggctc ttcccaaaat gccgtattat tgtgtgtctc tatgtcacga
      241 tctctcgttg cgttgatact catcgtcggc ttgtcattgg gcggcgcatt cgacgccttt
      301 gcacaatcgt cgaagcacca gcgcaaaacg aaaaagccca agtcgccgcc ctgccggacc
      361 ggctgcaaac cagacacgtc cgcacctcaa gttgccgccg atacgccgga agacgaggcc
      421 gcgcaaaaag aactttccga tctagcccgc gcgctgcaca acgccacgcc tgccgcatac
      481 gaaaaacttt cggccttcgc cgtcaagaac accacgaacg tttggggagc gcgtgccgcg
      541 cttgctcttg gctatgacga ctactcgaag aatcgcaccg cgcagggcct cgggtggcta
      601 atcaaagcgc agaacgacat gctgctacgc gagtatgctc tgtactggac cgcacaggcg
      661 cagcgcgctt tggggcgcag cgcggacgct tacaaagtgt tgcaatccat ccagcatgat
      721 taccccaata ctgccatgcg cgagcaactg ctggaggcct tcgcgcccac cgcggtccag
      781 ctaggacacg cgcaagaagc tatcgatgcg ctcgacgcct attccgccac cgcatcaaag
      841 cccacgcttc tttacgaacg tgcccacgcc tatcaggccg cgcatcaact tcctcgcgct
      901 gcaaaggact atcagaccgt cttctacaaa ttccccctgg cggatgaagc caagcccgct
      961 ggttccgctt tgtcgcagct gatgcacgcc ctgggaaaag aatatgctta tcccggcgtg
     1021 gaattgcagg agcagcgcgc ccaggctttt tacgatgtac acaaatggaa ggaagcacgc
     1081 gctgagtttg aaaaacttct caccatgctc aaggatgtcg cgaatcccgc gcgccagcgc
     1141 gcgcagcttc gagtcgcgga gtgccgcgtc cagctgaagg gttcaccttc cttgatcgcc
     1201 gcggtgaaga ccccggatct tgaagtggat gccgaacggc tctacgccct ttcccaagcc
     1261 taccgctccg caaaaaagga agcggaaatg ttttccgcgc tgaacactct ggcgcagaac
     1321 tatcccgtga gcaaatggaa tgaagaaggc ctgatggcgg cggcgaacta ccattgggtg
     1381 gaactcgagc gcacgaaagc ggtcatcaac taccagcgcg tcctggatac ctacccgaac
     1441 ggaaaatacg ccttcaactg cgaatggcgg atcgcctggg tcgcctatct gaatcgccag
     1501 cccgatgctg atgaccggct gactacattt ctattgaaat atcccgtatc gtccaactcc
     1561 gtcgatgccc tctactggct cgggcgcaac gccgagcgcg gcggaaatcc cgcgcacgcg
     1621 cgcagctatt acaacaaagc cgccgagcgt ttcccgcaaa cgtatttcgg caatgccgcg
     1681 gctgcccgcc tcgcaaagct tggtcccggc gaagaaaatc ccgcggagct gctggagaaa
     1741 atcccgccgc cgccttcgct tcgtccgttt gatgagccga tcccggttgc cgccgcggac
     1801 cgctgggcgc gcgcgcaggc gctccgcgcc atcgctttcg atgcctccgc cgagcaggag
     1861 ctaaagaacg cctacttcgc gacctcttcg ccgcgctttc tgctcgaagc cgcgcaagcg
     1921 gctttcgatc aaggccacta cggcgccggc atggcctacg cccgcatcat cgtgccgaac
     1981 ttcgattccc gcaagatcag cgatgtgcct gtcgccgcct ggaaggcgct ctttccgctc
     2041 ccctacgaag ccgcattgcg ccgcgaagcg gccaaaaatg atttcgatcc catgtttgct
     2101 gccggcctca ttcgccagga atccacgttt caggcggacg ccgtctcgca cgccaacgcc
     2161 atcggcttga tgcaggttct tccgaaaacg gggaagcttc ttgccaagca gctcaaagtc
     2221 cgctacacca aggacaaact tttcgagccg gactacaaca ttgaacttgg aatggtctac
     2281 attgcgagcc tggtgcgcca gacgggtgcg ctggaatacg cggccgctgc ttataacgcc
     2341 ggtgaagacc gcatcgccgc ctggaaagcc gagcgcaact acgatgagat ccccgagctc
     2401 gtcgagtcca ttccattcac ggaaacccgc gaatacgtgc aagtcgtctt gcgcaacacc
     2461 gaagtctacc ggatgattta tgggcagccg agcgtggcta cgcaggtcgc cattggccac
     2521 gcccgctgac gaattgtccg tattccgtga tctttccttt taggggcaca gcgcgttgcg
     2581 cctctgtggc tacgatttca tcgttctgag aaggtgcaat gactgctccc gacctctcga
     2641 tggcacaaat ctcccgccgc atgctttcgg caaaggccga gcaattcacc gaatccgtta
     2701 tccgggaaat gacgcgcctg gccttgaagc acggcgccgt caatctctcg caggggtttc
     2761 ctgactttgc cgcacccgag gaaatcaagg aagccgcgcg caaggccatc gccgatgaca
     2821 tcaatcagta cgccatcacc tggggcgcga agccgctacg cgatgccatt gtcgaaaaat
     2881 tcgagcgcac ccagggcgtc aagtatgacc ccgagcgcga ggtcactatc tgctgcggat
     2941 cgaccgaagc gatgatgtcc gccatgatgg ccatcatcaa tcccggcgac gaaatcgtcg
     3001 tcttcgagcc gttttacgag aactacggtc ctgacgcgat tttgagcggc gcgacgccgc
     3061 gcttcgtcaa gctccgcact cccgattggt cgttcgatga aaaggaactc gcggctgctt
     3121 ttggcccgca cacgaaagcg atcattctga acacgcccaa caatccgacg ggcaaagttt
     3181 tcacgcgggc cgagctcgaa tttatccgcg atctctgcgt ccggtggaac gcctactgca
     3241 tcaccgacga aatctacgag cacattctgt acgacggcgc cgaacacatc tccatggcgc
     3301 gcatcgacgg tatgcgcgac cgcaccatcg tcatcaatgg cctgtcgaaa acttacagcg
     3361 ttacgggatg gcgcgtgggc tgggtgctgg cgccaccgga accgacgcaa ccgattcgca
     3421 aggtgcacga tttcctgacc gttggcgctg ccgcgccgct ccaacaagca ggcgccagtg
     3481 ctcttcactt tccccagggc tactacgaca agcttgccac ttcttatgag gtcaaacgcg
     3541 aaaggctgct gaaaattctg acgagcgccg gcttcaccgt gttcaagcct cgcggcgcgt
     3601 actacatcat gacggatatt gcgcgcttcg ccgatcccga tcctattcgt taccaggccg
     3661 acacgaaaga tgtgcgcttc gccaagtttc tcgtcgaaac catcggagtc gccgtcgtcc
     3721 cgggctccag cttctacaac aacgcgcagg atggcgcttc ccaggtccgc ttcacattct
     3781 gcaaaaaaga agagaccctt gccgccgccg aggagcggct gtccaagttg cggccttgag
     3841 ccaatggttt gttgaaagcg atctgtggta acggaccaag gagatcctca tgcgaacttt
     3901 cttcacggcc atcgttttcc tctccgtgac atccttgctc gcacaggaca cccgcccgtc
     3961 tcgcgccttt gactacgatg ccaaggcgcc gctggatatt caggaagccg gggtggaaca
     4021 tcgcgagaac gtcgtcatcc acaacatctc gtacgcgagt ccaaagggcg gccgcgttcc
     4081 ggcatacctc gtggttccgg aggggaagag accctttgcc gccatccttt ggggacattg
     4141 gtactggcag aattctgctt tcttcaaccg caaggagttc ttggacgaag cggtcgcgct
     4201 agctcatacc ggtgtagttt ccctgctccc gaccgggccg ggggcgcgtg caggtcacgt
     4261 gcaggacaaa gatccattga acgatcagca gataacggag cttgttcaag ccattgttga
     4321 tatgcgccgg ggcgctgatc tgctgctcgc gcgcaaggat gtcgatccac agcgcttggc
     4381 ctatgttggt catagttaca acgccgtcgt gggcggtttt ctcagtggca ttgacaaacg
     4441 cttcaagtcg tttgtgctga tggccggagg actctcggat gaggtggaca tgaagaccga
     4501 ggatttccag cagttccgtc agaagatcgg cccggaaaaa gtagacgcgt tcaccgctaa
     4561 gtatgcctgg accgatcccg gcaaattcgt ttctcacgca acccccgcca ccgtgttcct
     4621 ccaatacgcc gcgcaggaga agtttctgac cgcggagcgc gcacgccagt atgcggccat
     4681 cgtgagcgag cccaagcagt tcaagctcta cgacgcccca cacgcgctca acgccgaagc
     4741 tcgccgcgac cgcatcgcct tcctttccga tcaactcaaa ctgaaaccgc tcgatccatc
     4801 gttggtaacg ggcatccccg atttgcctca accgcccgaa ccggctccgt agatgatgat
     4861 tcatgttcca agctccctga aaactgttgc gccagttgaa gtgttttcac tgcccattga
     4921 attggtcacc ttgtgctaga attcgcacgc aagaatccat tatgccgaca gaactagatc
     4981 tcattccgca cgatactcgc atggaagcca acggcgatgg cgccgcgttc gacatttctg
     5041 caagcgccac gcgcacgttc ttctgccgcc tcactgtcac ggatcagatt gaacaggaat
     5101 cgctcgatgt gtcgatctgg ggttctgccg acgcagagac ttggacgaag aagccgctcc
     5161 tcaagctgcc gcagcagttc taccgcggca cgaccaagat gatcctcgat ctctctttgc
     5221 gtccggagat caagttcatt cgtgctcgtt gggaagtgaa ccgttggggc agggtagcgc
     5281 cgacccccat gtttgtcgcc ggcctggagc tcgcggagat cccgccaatg tcccgcgaga
     5341 cccccacaac acgaatagcg cttgccgcga aataactcgc cagtctggaa ctatggcgtg
     5401 tacggggaat ccgccccgtt atttttggga ttgatttctt gcttctaaat tttatataaa
     5461 ataacgcagt ttcaagcgat ccatggtttt ttttaggggt aggagactga cggtcggttg
     5521 ccctgtggtg gttagccccc taccccgcga gtggtcttgc ctgtatagac gaggtgagcc
     5581 cccaaatagt ttcctgactt cctcaaaata atttgtggcc ctcccagttc ctattccccc
     5641 accggcactg tatctagata cctcgccacc gccgccttcc actgaaactt ttctgagatt
     5701 gctgcctgca acgcctgcgc cgcggccggc tcgccgtggg tcagatacgt ctgcttggga
     5761 ggcgcaggca gcgccgtcag ccagcgcaac aactcgctct ttcctgcatg tgcagagaat
     5821 tgccccatct ccacgatctc cgcgcacacc ggcacgacct gaccgtaaag attcagcgat
     5881 ttcgcgccct cttgcagagc tctcccgcga gtgccttcgg cctggaatcc cgccaggatg
     5941 accgcattgc gcgcatccgg cagccgttgc acgaggtgat gcagcacacg gccgccgctc
     6001 accatcccgc ttgccgagat gatgatgcag ggtttcttga cgttattgat ggccttggat
     6061 tcctccgccg agcgcgtcaa gtggaattca tgcacattta gaggatcgcc tttcccgtcg
     6121 ctgccttctt gccgcacata ctccaggtcg tgatcctctt tatgctttaa gtacaaatcc
     6181 gtcgcactca acgccatcgg gctgtccaca tacaccggca ggcgaggaat gcgctgctgg
     6241 tcctccagtt gccgcaggta gtacataaac gtctgcgtcc gcccgatggc gaacgacgga
     6301 atcacaatcg acccgccgcg tttcacgacg cggttcacga tttgcgccag caattccgcg
     6361 gggtcgcctt ccggatgttc gcggtctccg taggtcgatt cgcaaatcag cacatccgct
     6421 ttcgatggcg gcgtggtcgg gtccttcaaa atcgcctggt tgtatcgccc gatatccccg
     6481 gagaaaacca cgaccgtttt cctgccactc tcggtgatcg ttaactccag gctcgaagat
     6541 cccagaatgt gccccgcgtc atacgacgcc acgtgaaact ccgggctgac atcaaatccg
     6601 ccggctcgcg gcatgctctt cattagtttt agagtaggat caacgtcttc tgtcgtgtac
     6661 agcggctgcg gatcagggtg acggctgtat ttcttgagcc gtgcgtgctc ggcatcttct
     6721 tcctgcaagt gcgcggaatc tttcagcaat atggtggtca gttcgatcgt cgccggattg
     6781 gcgaaaatcg gcccgcgata tcccgatttc accagcactg gcagccatcc cgtgtggtcc
     6841 aggtgcgcgt gggtgagcac gagataatca atggtcttcg gatcgaccgg cagcggcttg
     6901 aagttgcggt ctttcagttc attcgtgccc tgaaaaattc cgcagtccac cagcagctgc
     6961 ttaccagccg cttcgaccag atacttcgag ccggtaacgc accccgccgc gcccagaaaa
     7021 gtgagttttg ccataggcgg ccattctaca gaaattgccc gcgttccgca gttgatccct
     7081 ggcgtctcgc tgaaagaaca aagcggagca agttttccgc tcgaacttgt tccgctcagt
     7141 ctttccggct aacaacgaac aacttacaac taagaactgt tttcaatccg ccgcgctggc
     7201 ctgcttcttc gcgacgatgg attcgatttt cagccccagc cctttcgccg cgccggtccc
     7261 atacgccgga tcagctttgt agaaatgctg gatttgcagt tcttgaatgc gcttcggcac
     7321 gccattgccc aagctggaga cgatgttgtc gatcagccgc tgcttcgcat ccgcgggcat
     7381 caagcggaag agatttcccg gctgtgtgta gtaatcactg tcggtgcggt gatcgtgccg
     7441 cgcgacggtt ccgctgacgg catgcggcca ttcgacgtac gaacggtcct gcgtgggacc
     7501 gccaaaactg ttcggttcat agttcaccga ggccccgccg ttttcatcga agcgcatcgc
     7561 gccgtctttg ttgtaggtgt ggaacgggca ctgcggtttg ttcacgggca gggtatcgta
     7621 gttgacgccc agccggtagc ggtgcgcatc gggataggag atcaagcgcg cctgcaacat
     7681 tttgtccggc gaaaagccca tgcccggtac gacattgcgc ggctcgaacg cggcctgctc
     7741 cacttctgcg aaataatttt ccggattgcg gttcaattcc agctcgccga cctcgacgag
     7801 agggtagtcc ttgtgcggcc agaccttcgt caagtcgaat gggttgatgt gataggtttc
     7861 cgcatccttt tccggcatga tctgcacgca cacgcgccat ttcgggaagt tacctgcctt
     7921 gatggcatgg aaaagatcgc gctgcgagta gtccggatcc gttccttcca acgcgcccgc
     7981 ctgctcacgg aagaaattct tgatcccttg cttggtcttg aagtgccatt tcacgtaaaa
     8041 gagctcgttc ttcgcagtga tcaggctgaa cgtatggctg ctgtagccgt tcatgtgccg
     8101 gtagccgtcc ggagtcccgc ggtcggagaa gagaatcgtc acttggtgca gcgattctgg
     8161 tgagagcgac cagaaatccc acatcatcgt cggcgatttt aggttggtct ccggatcgcg
     8221 cttctgagtg tggatgaaat ctccgaactt caacggatcg cgaatgaaga agacaggtgt
     8281 gttatttcct gtcatatccc agttgccctc ttcggtgtag aacttgagcg caaaaccgcg
     8341 gggatcgcgc gccgtatccg ccgaaccttt ctcgccgccc acggtcgaaa accgcaagaa
     8401 cgtcggcgtc ttcttgccca aggcaccaaa gagtcttgct gtcgtgtact tggtcatctc
     8461 ggcgctggtg cagacgaaat gaccgtatgc gcccgacccc tttgcgtgta ccacgcgctc
     8521 cggaattctt tcccggttga agtacgccat cttttcgaag agcagaaagt cttcgaagac
     8581 caccggcccg cgcggcccca ccgtcagtga attctggttg tcgccaaccg gacggcctgc
     8641 gtccgtggtc atgattttct ttttatctac cggcatggat cctcctcagg ttgatggcag
     8701 ttgccccgcg aactgaggcc gctgccattg cacctcgaag cctagagggt cacgacctat
     8761 acttccaata tatagttcgg ataggataga taggcatagc ctatggaact tcaccagctt
     8821 cgttacttct gcgcggtggc cgaaaccggt tctttcagcc gtgccgcgga acaatcgcac
     8881 atttcccagc cttcgctttc ccagcagatc ctcaagctgg aagacgagct tagcgcgcgc
     8941 ctcttcgacc gcctcggccg ctccgttcgg cttaccgagc ttggcaaaac gtttctcccg
     9001 cgcgcccgcg ctgtccttcg cgaactggaa gctgccaaag gcgacgtcgt cgaaggcaaa
     9061 gagttcatcg gcggtcccat caccgtcggt gtgattccca ccgttgcccc ttatttcctc
     9121 cctccgcgcc tcactacgtt ttcgcgcaaa tttccccagg ttcagctcac cgttgttgaa
     9181 gaaatcacac ccgtccttct cgaccgtctc cgtgcgggca ccattgacgt cgccatcttg
     9241 gcgctcccca tccgtggcca cgaattcgac gctgttcctc tgttgaccga gcggctcttc
     9301 gccgcgcttc ccaagaagca caaactcagt tcgcgcacgt ctctttccct caaagacctt
     9361 cgcgccgaac ccttcctgct cctgcgcgac ggccactgtt tccgcgatac cgccgttgcc
     9421 gcgtgcgacc gcgctcgtct ctatccgcag atcgttttcg aaagcggcca attcagcagc
     9481 cttctcagca tggttggcgc gggcatgggc gtttccatcg ttccggaaat ggccattgag
     9541 aagaaatctc aatgccgcta catccgcatc gccgatgatc aagcggttcg caccatcggc
     9601 gtagtcgtcc tccgcggccg ctctctgacc cgcgctcacc aatcttttct ttctctcgta
     9661 aattcctcta aggcttccag ctagcgcagt gccagcagcg ttcctttaat atgaccgtcg
     9721 tcacacagta ggagggcgcc cgtcacagaa ccacgttcct ctacgggtta gcctgtaaca
     9781 gaaaatgaca gcacagactc caccactcct cgaagctccc attccaccca agacggcggc
     9841 cgcgccgccg aaatcatcca aggcgaagaa aaaactcatc cgccgcaccg accgcgacta
     9901 ttcgcaattg gtccgccgta gctatcaggc tgcgtttctt ctttggaatg tctggttggg
     9961 aggcatcttt tatttctggg tctgcggaca tgaagcaggc actgccaccg tcgacgtggc
    10021 ccctccaccc ggcatcgaag gctggctgcc gattgccggt ctcatgaatc tgaaatactt
    10081 cttgctgacg ggcctggttc cctcgattca tcctgctgcg atgtttctcc tcgtgacttt
    10141 tcttgccatg tcgtttcttt ttcgcaaggc attctgcagt tggctctgtc cggtgggcac
    10201 cctttctgaa tacttgggcc tgcttggcaa gaaaatcttt ggccgcaatt ttcgtgtctg
    10261 gcgctggatc gatattcctc ttcggggatt gaaatacttc ttgctggggt tttttgtgtg
    10321 ggccatctca tccatgtccg ccgccgcgat cgccaacttc atgcgcagcg attatggttt
    10381 ggtcgcgggc gtaaagatgc tgaatttctt ccgccatctg ggcgaaaccg gcttgatcgt
    10441 tgtcggtatc ctcgtgctcg cctccgtctt cgtgcagaat ttttggtgcc gctacctttg
    10501 ctcttacggc gctttgctgg gatttgcttc attactgagc ctcgtgcgca ttcgccgcga
    10561 gcccgaacct tgcattgatt gtggcaaatg cgccaaagtg tgcccctcca gcttacccgt
    10621 cgataggttg gtgaccatca agtccgccga gtgcacggga tgtctggaat gcgtggccat
    10681 ctgtcccgct gagggcgcgc tgcaccttgc cttgccgcgg ttccgcaatc cacctagacg
    10741 aatcccgtcc tgggttatgg ctgcggggat cgccgttctc ttcttcggag tcgtcggttt
    10801 cgccaaggcc gttggcttgt gggaatcgca tgtgccaggg gccgtctatc ggcaactcgt
    10861 gccgcatgca agtcaagccc gccatcccat gcccggggat cctgaaatct ccaggtagtt
    10921 gcgaaacatc gattcgacgt ttgtgctgga atggacagcg attttggtgg agccgatggg
    10981 attgtcaatc gcctcttgaa gaaaacccaa agaaaaccgc taaatctacg agtttattgc
    11041 acttactgat ttttccaatg tcccttagaa acccttagag gttctttgga cacccgtata
    11101 atgccccatt ctggcaacag ttggcaacag ttggtggtgg gtctgtttga agtagtgcag
    11161 tgcagagggg ccgctatttc ccagatccgc gtttg
//
LOCUS       DASYVO010000339         1986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_167030, whole genome shotgun sequence.
ACCESSION   DASYVO010000339 DASYVO010000000
VERSION     DASYVO010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167030"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /gene="asnB"
                     /locus_tag="VGR03_11510"
     CDS             complement(<1..1344)
                     /gene="asnB"
                     /locus_tag="VGR03_11510"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEV1994949.1"
                     /translation="MCGILGSILGNTGSPDPGRFNLALGLLEHRGPDRRSFLVVNGSG
                     THRLFGSLDSTLEPAGGQIVLGFCRLAILDLSSAGDQPMTSPDGLLHIVFNGEIFNYL
                     EIREELVQLGWRFRSQSDTEVLLAAYQQWGADCLGKLLGMFAFAVADMKRRVLFLARD
                     HLGIKPLYYTFASGKGAFSFASQVAALLKVSSLPPVAQSGALGDYLLRGHIDHTQATL
                     FRGVEQVLPGHYLEIGMDQTLPAAQVRYWSAPAGGRSELSASEATEQFRGHLEDSVRL
                     HLRSDVPVASALSGGMDSSSIVSLMRKLQPPDTPIHTFTFVTRGCPGFDPAWDEEKWA
                     DIVGQSARAVMHKVRVDPHQLPQDCERFLLLQDGPFGSPVVWVQQKIFQAAHAAGFRA
                     MMSGQGADELVAGYRRHIAARLASLLRQGRMGKYLRLLRAGNRQPNPEGLGLRSAS"
     gene            1478..1750
                     /locus_tag="VGR03_11515"
     CDS             1478..1750
                     /locus_tag="VGR03_11515"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEV1994950.1"
                     /translation="MSSQFIQAPNVEVAPMHGETVLFNPDSNKFCLLNQTAALVWSML
                     EEPVTTDAMAAEICNRYGGTEPAKVLQDVREVIDRMQKLNFVVPKD"
     gene            1776..1928
                     /locus_tag="VGR03_11520"
     CDS             1776..1928
                     /locus_tag="VGR03_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994951.1"
                     /translation="MENTGRSITLDAKAESKPAYVPPMIKLMEEKDLLAEFQVSVNAN
                     SWWAAM"
BASE COUNT          438 a          595 c          612 g          341 t
ORIGIN      
        1 ggatgcggag cgcaaaccca agccctccgg attcggctgc cgatttcccg cccggagaag
       61 acgcaggtat ttgcccatcc ggccctgccg cagcagcgag gcaaggcgcg cggcgatgtg
      121 gcggcgatat cctgccacca actcgtcggc cccctggccg ctcatcatgg cgcggaaacc
      181 ggccgcatgc gccgcctgga agatcttttg ctgcacccac accaccgggc ttccaaaggg
      241 accatcctgc aagagcaaga aacgttcaca gtcctgcggc agctggtgcg ggtccacccg
      301 cactttgtgc atcaccgccc gcgcggactg gcctactatg tccgcccatt tctcctcatc
      361 ccacgccggg tcgaagccag gacagcctcg cgtgacaaag gtgaaggtgt ggatgggggt
      421 atccgggggc tgaagcttcc gcatgagcga gacgatggag gacgaatcca tgccgccgga
      481 aagggccgag gccaccggca catcgctgcg tagatgcagc cggacggaat cctccaaatg
      541 gccgcgaaac tgctctgtgg cttcgctcgc tgaaagctcg ctgcggcctc ccgctggagc
      601 actccaatat cggacctggg cagccggcag ggtctggtcc atgccgattt ccagataatg
      661 cccggggagc acctgctcca ccccgcggaa caatgtcgcc tgcgtgtgat cgatgtgccc
      721 gcgaagcaga tagtctccca aggcgccgga ttgagcgacc gggggcaggc tcgagacttt
      781 gagaagggca gcgacctggg aggcgaatga gaatgcgccc ttgccggacg cgaaggtgta
      841 atagagcggc ttgattccca gatggtcgcg cgccaggaac aggacgcgac gcttcatgtc
      901 ggccacggcg aaggcgaaca tccccagcag ctttcccaga caatcggcac cccactgctg
      961 gtaggcggcc agcaatacct cggtatcgga ctgggagcgg aatcgccatc ccagctggac
     1021 gagttcctcc cggatctcca ggtaattgaa gatttctcca ttgaatacga tatgaagaag
     1081 gccatcgggg gatgtcatgg gctgatcccc cgccgagctc agatccagga tggcgagccg
     1141 gcaaaagccg aggacgattt gccccccggc cggctccagg gtcgaatcca gagacccgaa
     1201 aaggcggtgt gttccggacc cattgaccac caggaaagag cgacggtcag ggccgcgatg
     1261 ttccaggagg cccagcgcca ggttgaaccg gccagggtcg ggggagccgg tattccccaa
     1321 gatggaaccg aggatgccgc acatgggcgg cccaagaata gcacgcggct tcagacccga
     1381 tttccaaggg catggggacc tatttcaatc ttgacaattc cccagcctct tgcctagaat
     1441 cacgccacgg cagaccagct ctcagtcagg atgtgctgtg agttcgcaat tcattcaggc
     1501 acccaacgtt gaagtcgctc cgatgcacgg cgagacggtg ctgttcaatc cggattcgaa
     1561 caagttttgt ctgctcaacc agaccgcggc tctggtttgg tccatgttgg aagagcccgt
     1621 gacgacggat gccatggcgg cagagatttg caaccggtac ggaggcacgg aacccgccaa
     1681 ggttttgcag gatgttcggg aagtgattga ccgtatgcag aaactcaact tcgtggtgcc
     1741 gaaggactaa ctacaatcac taaggagact tatcgatgga aaacacaggc aggagcatca
     1801 ccctggacgc gaaggcagaa agcaagccgg cctacgttcc tccgatgatc aagctgatgg
     1861 aagagaaaga cttgctcgcg gagtttcaag tctccgtcaa tgccaactcg tggtgggcag
     1921 cgatgtgatc cctgccgaac tacggcaatg gggctcgaaa gagttcttga gccagcaagt
     1981 gcccgg
//
LOCUS       DASYVO010000340        10706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_248546, whole genome shotgun sequence.
ACCESSION   DASYVO010000340 DASYVO010000000
VERSION     DASYVO010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 10706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10706
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248546"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VGR03_11525"
     CDS             complement(<1..450)
                     /locus_tag="VGR03_11525"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1994952.1"
                     /translation="MPNPVRAIPEGYHSISPSLTCKNAASAIDFYKSVFGATEIMRMP
                     DPQGLIMHAEIRIGDSVVFINDEIPGMASAPSGASLNPVSLFLYTEDVDAVFNRAVTA
                     DCKVTMPLANMFWGDRYGKFDDPFGHSWGVATHVADLSPEEMMRGQAE"
     gene            851..1543
                     /locus_tag="VGR03_11530"
     CDS             851..1543
                     /locus_tag="VGR03_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994953.1"
                     /translation="MTIETKIVRLCVSALLAFAIGKLVFAPLFFVLWPGLYLGWHFLN
                     RHRKSPELRAPRSIEGQKSPAVAVTNRRYMLGRIILVAGVVIVGWVAVSILFLFLARE
                     SGHRKAEKARSTVHPGMNVAEVLHSVTGWIILGASSDAPETAPGHLSAVSLGFHPENN
                     TFIYFDGMKGADRELSESETLVLLHQKLGDGYSWRFRFTFVSSTPQHFSFKVEFDKDG
                     RVREVKPVYGWD"
     gene            1656..2267
                     /locus_tag="VGR03_11535"
     CDS             1656..2267
                     /locus_tag="VGR03_11535"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV1994954.1"
                     /translation="MRYDFLVGTYETERMKVVSVRSEFRDEDLPVRLREGDPRGRSVH
                     EQMVHQCVSENLWFINMLGIDVNAPPLPATETRLEFMKRYAEDSGKRLAALRAKDDVW
                     WESETKFFAVQRSRAWVMVRRIAHTAHHRGQQMGMLRMLARDLHSNYGPTADTGGLMQ
                     NHAPTIYGYSSVNALLEGEAAGGTKTPLPGAAGKPVTERPDKK"
     gene            complement(2293..2808)
                     /locus_tag="VGR03_11540"
     CDS             complement(2293..2808)
                     /locus_tag="VGR03_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEV1994955.1"
                     /translation="MKSLACAALFLSAAGLVFAQESRAHAAAKPVTVDFKNVQGQSVG
                     TATLTPAAKGVKITIDIKNLPPGEHSIHIHQVAKCEPPDFKSAGPHFGGGGHVHEGMP
                     AGDIPDFALIVAADGTAHASTNAPNVTLGTDANSVFSNGGTTIVIHAVAGGTGSGAPP
                     RIACGVITKPE"
     gene            complement(3032..3724)
                     /gene="msrA"
                     /locus_tag="VGR03_11545"
     CDS             complement(3032..3724)
                     /gene="msrA"
                     /locus_tag="VGR03_11545"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEV1994956.1"
                     /translation="MQSPSIGRGIALVVIVVLVGWTAFRALGSRPPETPKIPFPAPSM
                     NAPLATAKSQQTAVFAGGCFWGVQAVFEHLKGVSSVTSGYAGGYVKSPSYESVSMGVT
                     GHAETVSITYDPSQLTYGQLLMVFFSVAHDPTQWNRQGPDTGSQYRSAIFYTSAEQKR
                     IAQAYIAQLDAAKVYSRPIVTKVEPFEAFYPAESYHQDFLKNNPDNPYIVYNDLPKLE
                     NLKKIFPILYQP"
     gene            3943..5136
                     /locus_tag="VGR03_11550"
     CDS             3943..5136
                     /locus_tag="VGR03_11550"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEV1994957.1"
                     /translation="MRARNILALAFPFFLPSLATAQVTHGQKPKLPVPFATNSSGNGP
                     GKEKPPAGFLPTVPPGFRLNVYAADFKRPRWLTVAPNGDIFLADNGAGEIIVMRDPQH
                     TGAAQEREVFASGMRRPFGIAFHEDYVYVGNTNELVRFRYDSKTSKRLGEKEHLMDLP
                     TGGHDTRALAFSADGKHLFIAVGSGSNIDTGEEPRRAAVTICDPDSKNARLYATGLRN
                     PVGLALEPVTGEVWTSVNERDELGDDLPPDYFTSVKDGGFYGWPYSYIGDNVDSRVKQ
                     EHPELVARAIIPDVLLGAHVAPLQFAFYTGKQFPESYRGGAFVAEHGSWNRATRSGYQ
                     IAFVAFKNGKPSADPVPFMTGLVPDPSKKDVYGRPVGVAVAPDGSLLVSDDGAGVVYR
                     ISVAK"
     gene            complement(5221..7467)
                     /locus_tag="VGR03_11555"
     CDS             complement(5221..7467)
                     /locus_tag="VGR03_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEV1994958.1"
                     /translation="MTRNDQTGVLTPVSGSPFAPAVFQTLDVQGRFLFGPGTNAIHMY
                     QVNSTTGVYSEVPNSPFASPNTNQPVFIAVEPSGKYLAVVNQVGLNPGESSIETFQID
                     AANLALVPVLGSLLELDSTLIGAEADPKNRRFYVFLAPNPSNPLLQLNSELNDYAIDP
                     LTGFVSGNIFTVGTIGHCLAIDPQARFLVIGMGRIAGIIGVLPIAAATGIPSSALATT
                     SLGLGVFPTAVSTEVTGNFIYLTDTGTSPGPVHIYALDSQTLTVAETSSSPLPGFTSV
                     PGLMGDPTGPFMYGLVGTSVQAYMTDPQTGYFTPVSGTFISAPGVNGNFVFSIPPGQQ
                     NLVGPVATLTPSSLSLGNILVGTPSQAQSITLTSTGDQGLSLSSISIAGANANEFSES
                     DNCQAPTVLQPSKFCIISVIFTPSATGQRQATLALLDNAPGSPQSVPLNGMGVVPPPP
                     NPLVTLIPGSISFPTITQGMKGTPSTVTVTNSGNATLHIASVVVGGNNPNDFASTINC
                     SGAISANASCTITVTFAPLAAGQRTETITLTDDAPNSPQIVNVGGNANPAFSGGPAPN
                     GSTTASVSAGQTAQYLLQLNPGAGYSGTVSLTCSGAPLNAACQVPASVSIANGAPAPF
                     TVTVLTRGGAVLPPSIPWRLVPPAGIRVLLLLALAWVLVKAGKNRWMFDSTLRERRLA
                     WSGALAAVFLCSVIFAAGCGSSSVTTTPPPVVTPPGSSTITITPTAMSTSGMPLQLQP
                     IQLTLTVK"
     gene            7893..8225
                     /locus_tag="VGR03_11560"
     CDS             7893..8225
                     /locus_tag="VGR03_11560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV1994959.1"
                     /translation="MDLEKQAESNSSHLVMRLNGKLSLETVHNFIQTLRPEPAAHLIL
                     DLSGVSFLDSAGVGALVQLFVHRRNLGQTFALTGLTKQSGAVIQVAGLTKLLPIHASV
                     AEALAQRA"
     gene            complement(8239..9051)
                     /locus_tag="VGR03_11565"
     CDS             complement(8239..9051)
                     /locus_tag="VGR03_11565"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994960.1"
                     /translation="MTSGLAILTIGFFLGMRHATDPDHVIAVSTIVSRERSIARAGLI
                     GILWGCGHTLTIVAVGTAIILFGLAIPVRAGLTMEFSVGLMLILLGVLNLTGAMKWIS
                     EKFSPAHPRVTGEHAHLHEHDSHHVHFHWHSHALAAEHHGDSLPAPSWLRGPFEQLGL
                     FHALRPLFVGIVHGLAGSAAVALLVLSTIHNPKWAVLYLLIFGVGTIAGMMLITAALA
                     LPFSFTGSRFAWLNRSFVIGSGLLSLSFGLFVCYQISFAGGLFTNHPHWTPS"
     gene            complement(9148..10023)
                     /locus_tag="VGR03_11570"
     CDS             complement(9148..10023)
                     /locus_tag="VGR03_11570"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1994961.1"
                     /translation="MDLHELQVFLMVAKEGSFSRAGERLYRTQPAISLAIRKLEDSLG
                     QPLFVRGARPVRLTDAGTLLKDYAERLINLRDEVKKGLSELEGLKRGELSLGVNESSI
                     HALLPALAKFREKHPGVQVRVHRMFSRDIPHEVLNYRLDLGAISFVPRDAQLQATEIL
                     KDELTLVVPPKHILAKKREVDVAELGEEIFIAHSVESPFRRRVIELFARNRTALNMPI
                     EMPTIESIKRFVQMGMGVAIVPRMCVQWEIEHGWMKEVKVRQLNMPRHVYLVSRRGAR
                     LPHAASELMRILREE"
     gene            10130..>10706
                     /locus_tag="VGR03_11575"
     CDS             10130..>10706
                     /locus_tag="VGR03_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994962.1"
                     /translation="MDVVRIFDTTLRDGEQSPGFSMNTEEKIRLARQLAALNVDVIEA
                     GFPIASKGDLEAVKKVAQEVRTVPIAALARAKKEDVSAAIEALKSAAAPRLHIFLATS
                     DLHLNVKLKMTREQALEAISSMIRYGRQHVAEVEFSAEDAGRTDIDFLCQVCHVAVDA
                     GATVLNLPDTVGYAVPEEYGAMFTKVREYLGD"
BASE COUNT         2377 a         2808 c         3397 g         2124 t
ORIGIN      
        1 ctcggcttgg cctcgcatca tttcttccgg agagagatct gccacgtgcg tggcgacgcc
       61 ccaggaatgg ccgaacgggt catcgaattt accgtagcgg tcgccccaga acatatttgc
      121 aagcggcatg gttactttgc agtccgccgt aacggcgcga ttgaacaccg cgtccacatc
      181 ttccgtgtaa aggaagagag agacaggatt gagcgaagcg ccgcttgggg cggacgccat
      241 gccaggaatc tcgtcgttga tgaaaacgac agagtcgcca atgcggattt cagcgtgcat
      301 gatgaggccc tgagggtcgg gcatgcgcat gatttccgtg gcgccaaaga cggacttgta
      361 aaaatcgatg gcgctggcgg cgttcttgca agtgagcgac ggcgaaatag aatgatatcc
      421 ctcagggata gcacggacag gattcggcat attgacctcc tagacgttcg gatgtgcgca
      481 cgctggaaac gggagggagc aacagaacag cgcgcgtaag ggtgtaaata ctcctccgaa
      541 cggctggtgt caacaggttc aggtgccccc tcgaaacgtc ccgacaaggc aggggcaggc
      601 cgggcagact gggcaagctg gttactcgtg accggagact cccgtaaatc gctggttgcg
      661 ccgactcgtt gctcgtccgg tgtgagaaat taaccagcta ctctagaaag tggactaccc
      721 gacaggttcg aggtgaaccg ggatgccatt ttccggacct cgttactcac tcgcgtcgac
      781 tctgaagcat tgcggcctgg tttccgggca ggattgtaag ctcgcagtca tatagggccg
      841 cggagttgcc gtgactatcg aaacgaaaat cgtccgcctc tgcgtgagtg cgttacttgc
      901 gttcgccatc ggcaaactgg tcttcgcgcc gctgttcttc gttctttggc ccggactcta
      961 tttgggatgg cacttcttaa accgccaccg caaaagcccc gagctgcgag caccccgatc
     1021 catcgagggg cagaaatcgc ccgctgtcgc cgttacaaac aggcgttaca tgctaggacg
     1081 catcattctt gtagcgggcg tcgtgattgt ggggtgggta gcagtatcca ttctctttct
     1141 gttccttgct cgggagtcag gtcatcgaaa ggcggagaag gcccgcagta cggtgcaccc
     1201 gggcatgaac gttgctgaag tgttgcattc agtgaccggt tggatcatac tcggagcgtc
     1261 ctccgacgca cccgaaactg ctcccggcca tctttccgcc gtaagtctgg gcttccatcc
     1321 ggaaaataac acgttcatct atttcgatgg gatgaaaggc gcggaccgcg aactctccga
     1381 gtctgagacc ctcgtccttc ttcatcaaaa gctcggcgac ggatatagct ggcgattcag
     1441 attcaccttc gtcagcagca caccgcaaca cttctcattc aaggtggaat tcgataagga
     1501 tgggcgcgtc cgagaagtca aaccagtata tggttgggat tagcgccgcc gtcgatttcc
     1561 tgtcttggtt gacggcctct gagtgacagc caatttgacg tggctttttc cgggagagaa
     1621 aatgcacgcc gaagccgcgc gggaggatgg cgtccatgag atacgacttc ctggtgggaa
     1681 cctacgagac cgagcgcatg aaagtggtga gcgtgcggag cgaattccgc gacgaggatc
     1741 ttcccgtgcg tctgcgggag ggggacccgc gagggcgcag cgtgcacgaa cagatggtgc
     1801 accaatgcgt gagcgagaat ctgtggttta tcaacatgct ggggattgac gtcaacgcgc
     1861 cgccattgcc ggcgacggag actcgattgg aattcatgaa gcgctacgcg gaagacagcg
     1921 gaaagcggct cgcggcgctg cgggcgaaag atgatgtttg gtgggagtcc gaaacgaaat
     1981 tctttgccgt gcagcgatcc cgggcgtggg tgatggttcg gaggatcgcg cacaccgcgc
     2041 accatcgagg ccaacagatg gggatgctac ggatgctggc gcgggatttg cacagcaatt
     2101 acggcccgac ggcggatacc ggaggactca tgcagaatca tgcaccgacc atctacgggt
     2161 attcgagtgt gaacgcgcta ctcgaaggag aagcggctgg tggaacgaag acaccgcttc
     2221 cgggcgccgc cggcaaaccc gtgaccgagc gcccggacaa gaaatagatt tgaaaattgt
     2281 cgcaggccga atttattcgg gcttggtgat cacaccgcag gcgatgcgcg gcggagcacc
     2341 gctgccggtg ccgccagcga cggcgtgaat gacaatcgtg gttccaccat tgctgaagac
     2401 ggagttagcg tccgtgccta gagtcacgtt cggcgcgttg gtgcttgcgt gggccgtgcc
     2461 atccgcagcg acgatcagcg caaagtccgg gatatcgccc gcaggcatac cttcatggac
     2521 gtgcccgcca ccaccaaagt gtggaccggc ggatttgaag tcgggaggct cgcacttggc
     2581 gacctggtga atgtgaatgg agtgttcacc cggcggaaga tttttaatat cgatggtgat
     2641 tttgacgccc ttggccgcgg gcgtcagagt cgcagtgcca acgctctggc cttgcacatt
     2701 cttgaagtcc acagtgacgg gctttgcggc ggcgtgtgca cggctctcct gcgcaaatac
     2761 cagaccggct gccgacaaaa acagcgcggc acaagcgaga gatttcatgt tgggtctcct
     2821 cacggcgaat ggtgtttcag gattaggact ttctcagaag aacgccccat ttcaatctag
     2881 cgcaacgccg cagaggacgt aacaacttgt cctcaaatag atagccttca tagctgggag
     2941 gaatgcgccc tcggggaatg agtattctcg cgagatttcg agagaaatag aaaagagtcg
     3001 gaatcggacg gtcgcgaaag gtttttcggt attaaggctg atagaggatc ggaaaaattt
     3061 ttttcagatt ttccaatttg ggcaggtcgt tgtagacgat gtagggattg tcgggattgt
     3121 tcttgaggaa atcctgatgg tagctctcgg cggggtagaa ggcctcgaaa ggctcgacct
     3181 tcgtgacgat gggccgggag tagaccttcg cagcatcgag ctgcgcgatg taggcctgcg
     3241 cgatgcgctt ctgctccgca ctggtgtaga agatcgcgga gcggtattgg gagccggtgt
     3301 ccgggccctg gcggttccat tgcgtgggat catgcgcgac ggagaaaaaa accatcaaga
     3361 gctggccgta ggtgagctgt gacggatcgt aggtgatgct gacggtttcg gcgtggccgg
     3421 tgacgcccat gctcacggat tcgtaggacg gtgacttgac gtagccgccc gcatagccgg
     3481 aggtcacgga agaaactccc ttgagatgtt cgaagacggc ctggacgccc caaaagcagc
     3541 cgccggcgaa taccgcggtt tgctggctct tggcagtggc caacggcgca ttcatggacg
     3601 gggcggggaa tggtatcttc ggggtttcgg gaggccgcga gccaagagca cggaaagcgg
     3661 tccagccgac caggacaaca atcaccacta aagcgatgcc gcggccaata gaggggctct
     3721 gcataatccg tctccctgaa cacccagctt agtttaccct tcctatgctg cattggatgc
     3781 ggatcgaagc gtgttggatg agggaagatt cgccttcatt cggcagctcc catctggttc
     3841 gccggcgact ccatggcgac gcggcggaaa ttggaaccga gtgcccgcct gaggcatcat
     3901 agagagcgaa acgatcatcg tgttttctga aaggctacca ccatgcgcgc acgaaacatt
     3961 cttgctttag ctttcccgtt ttttctaccg tccctggcga cggcgcaagt tacgcacggc
     4021 cagaagccaa agctgccggt gccgttcgcg acgaattcat cgggcaatgg accgggcaag
     4081 gagaagcctc ctgcgggttt tctgcccacg gtgccgccgg gatttcgctt gaacgtatat
     4141 gccgcggatt tcaaacgtcc gcgctggctg acggtagcgc cgaacgggga tatttttctc
     4201 gccgacaacg gagcgggaga aattatcgtg atgcgcgacc cacaacacac cggcgcggcg
     4261 caggaacggg aagtatttgc gagcggtatg aggcggccgt ttggaatcgc ctttcatgaa
     4321 gactacgtct acgtggggaa caccaacgag ctggtgcgtt ttcgctacga ttcgaagaca
     4381 tcgaagcggc tcggtgaaaa agagcatctg atggacttgc caacgggcgg gcatgacacg
     4441 cgagcgctcg ctttttccgc ggatggaaag cacctcttca tcgctgttgg atctggttcg
     4501 aatattgata cgggagagga gccgcgccgt gccgctgtga ccatctgcga tccggacagc
     4561 aagaacgcgc ggctgtacgc gacgggattg aggaatccag tggggctggc gctggagccg
     4621 gtgacgggag aagtgtggac gagcgtcaat gaacgcgacg agctggggga tgacctgcct
     4681 cccgattact tcacgtcagt gaaagatggc ggattttatg gctggccgta cagctacatc
     4741 ggggacaacg tggattcgcg cgtgaagcag gaacacccgg aactcgttgc gcgggccatc
     4801 attcccgatg tattgcttgg tgcgcatgtt gcgccgttgc aatttgcgtt ttatacgggg
     4861 aagcaatttc cggagagtta ccgaggcggt gcgttcgtgg ccgaacacgg atcgtggaac
     4921 cgagcgacgc gctcgggata ccagattgcg tttgtggcgt tcaagaatgg caagccatcg
     4981 gctgaccctg ttccgttcat gaccggtctg gtgccggatc cgagcaagaa ggacgtatat
     5041 ggacggccgg tgggggtggc cgtagcgccg gacgggtcgc tgctggtgtc ggatgacggg
     5101 gccggcgtgg tgtaccggat ttccgtagcg aaataatccg gccgagcggc gaaggcttcg
     5161 gcatttgcca gggcgatttc cctcagaaaa acagcgcacg ctaaaatctg ttttacggcg
     5221 ctacttcacg gtcagcgtga gctgaatggg ctgcagttgc agcggcatcc ccgacgtaga
     5281 catcgcggtt ggcgtaatcg tgatcgtcga acttcccggt ggcgtgacga cgggtggcgg
     5341 cgtggtcgta acgctgctgc tgccgcagcc cgccgcaaaa atcaccgaac aaaggaaaac
     5401 cgcggcgaga gcaccgctcc acgccagacg cctctcgcgg agtgtgctat cgaacatcca
     5461 gcgattcttg ccagctttca ccagaaccca tgcgagcgcc agaagcagga gcacgcgaat
     5521 tcctgccgga ggaacaagcc gccacggaat cgatggcggc agcacggcgc cacctctggt
     5581 taacacggtt accgtgaacg gtgccggggc gccattcgca atcgacacac ttgcagggac
     5641 ttggcacgcc gcgttgagcg gcgcgccgct gcaagtgagc gacacggtgc cgctatagcc
     5701 ggctccggga tttagctgca gcaggtattg cgcggtctga ccggccgata cggaagcggt
     5761 cgttgaaccg ttaggagctg gcccacccga gaatgcggga ttggcgttgc cgccgacgtt
     5821 aacgatctgc ggcgagttag gggcatcgtc cgtaagtgtg atggtttcgg tgcgctggcc
     5881 agcggcgagc ggagcgaacg tcacggtaat cgtgcaactt gcattggcgg aaatcgctcc
     5941 ggagcagttg atcgtggagg caaagtcgtt tggattattt ccgcccacga cgacggaggc
     6001 gatgtgaagt gtcgcattgc cggaattcgt tactgtcacc gtggaaggag tgcccttcat
     6061 tccctgcgtg atagtcggaa atgaaatgct gccgggtatt agcgtgacca aggggttggg
     6121 cggtggcggg acgacaccca tgccattgag cggaacggac tggggactgc ctggagcatt
     6181 atccaacagc gccaacgtcg cctgccgttg gcccgtggct gacggtgtga agatcacgga
     6241 gattatacaa aacttactcg gttgcagaac tgttggcgcc tggcagttgt cggattcgct
     6301 gaattcgttc gcgttggcgc cggctatcga aatcgaactg aggctcaatc cctggtcgcc
     6361 tgtgctggtg agagtgattg attgtgcctg gctgggcgtg ccaaccaaga tgtttccgag
     6421 ggagagcgaa gaaggagtga gagttgcgac cggcccaaca agattttgtt gtccgggagg
     6481 gatgctgaag acaaagttcc cattcacgcc aggcgcagaa atgaatgttc ctgagacggg
     6541 agtgaaatac cctgtttgcg gatctgtcat gtaggcttgg acggaggtgc caacgaggcc
     6601 gtacataaac gggccggtgg ggtctcccat caatccgggc acggaagtga aaccgggcaa
     6661 aggcgaactc gaggtttccg ccacggtcag cgtctgcgag tcgagcgcgt aaatatggac
     6721 aggtccaggg gaagtgcccg tgtcagtgag atagataaag ttcccagtta cctccgtcga
     6781 aaccgcggtc ggaaagacgc ctagacccag cgaggttgtc gcaagcgcac tgctgggtat
     6841 accagtggct gccgcgatgg gaagaacgcc gattattcct gcaatccggc ccatgccgat
     6901 gacgagaaag cgggcctgag gatcgatggc aagacaatgg ccgatcgttc ctaccgtgaa
     6961 tatgttcccg ctcacgaagc ccgtcagcgg gtcaatcgca tagtcgttga gttccgaatt
     7021 caactggagt agagggttag agggattggg ggcaagaaag acataaaaac gccgattctt
     7081 ggggtcggcc tctgcgccaa tgagcgttga atccaattcc agcaaagatc cgagaacagg
     7141 aacgagggcg agatttgcgg catcgatctg gaaagtttcg atcgagcttt ctcctgggtt
     7201 gaggcctacc tgattaacga cagcgagata ctttccgctt ggttcgacgg cgatgaagac
     7261 gggctgattc gtgttgggcg aagcaaacgg agaattggga acctcggaat aaactcccgt
     7321 tgttgaattg acttgataca tgtggatagc gttcgtaccg ggaccaaaca agaaacggcc
     7381 ctgaacgtca agcgtttgga acactgccgg agcaaacggc gacccagaaa caggcgttag
     7441 cacgccggtc tggtcgtttc gcgtcataac agctcccgca gtggagaaga cgaagggctg
     7501 ctggaactga gcgtgtgccg agatggaaca gatcaataga ccaatcaaca aggcaagagg
     7561 gcggagtgcg ttccagcatc ctgcgcggcc tacgtgggac gctgcgggcg gaagggtact
     7621 gaataggtct tgtgtggcca tgagtcttcc ttggccgtca gttgagcggc gcattcttga
     7681 acctgagagg gaaacagttg caatttcacg ttaacgccgt cgagcgcgag cgtcaatacg
     7741 aattagctaa atagataagg atagcgagta gttcaatcgg aaaagtagaa gagcggggga
     7801 agggtgcagc gcacgagacg aattgttaaa gaattgtaat gtgtggcagg caggtattga
     7861 gctaccctcc gtatctcatg gaaccaactc acttggactt ggaaaagcag gcggagtcca
     7921 acagcagtca cctcgtgatg cggctcaacg gcaaactgag cctcgagacc gtccacaatt
     7981 tcatccaaac cttgcggccg gagcccgcgg ctcatctcat tcttgacttg agcggcgtgt
     8041 cgtttctcga ctccgcgggc gttggggcgc tggtgcagct cttcgttcac cgcagaaacc
     8101 tggggcaaac gttcgcactg acgggactga cgaagcagag cggcgcggtg attcaggtag
     8161 cggggctgac caagctcctg cccatccacg cttcggtggc ggaagcactc gcacagcgag
     8221 catagggcgc gttgaagatc agcttggcgt ccaatgagga tgattggtga agaggccgcc
     8281 ggcgaaactt atctggtaac agacaaatag cccgaaactc aaactgagga gacctgatcc
     8341 aatcacgaag ctgcgattga gccacgcgaa tctggaaccg gtgaaagaaa acggcagggc
     8401 gagtgccgcg gtgatcagca tcattccggc gatagtgcca acaccgaaaa tcagcaggta
     8461 gagaaccgcc catttcggat tgtgaatcgt gctcaacacc agcagagcca ctgcggcgga
     8521 acctgccagg ccatgaacga tgcccacgaa cagagggcgc aaggcgtgaa aaaggcccag
     8581 ttgttcaaaa ggtccgcgca gccaactcgg cgcaggcagg ctatcgccgt gatgttccgc
     8641 ggccagggcg tgagagtgcc aatgaaaatg cacgtggtgg gagtcgtgct cgtgaaggtg
     8701 ggcatgctct ccggtcacgc gcgggtgggc aggggagaat ttctcgctga tccatttcat
     8761 cgcgccggtg aggttgagca cgccgagcag aatcagcatc aggcccacgg aaaattccat
     8821 ggtcaggccg gcacgcacag gaattgccaa gccaaataaa ataatggccg tcccgacagc
     8881 gacgatggtc agcgtgtggc cgcagcccca gaggatgccg atgaggcctg ctctggcaat
     8941 cgagcgttcg cggctgacga ttgtagacac ggcgatcacg tggtctggat cggtggcatg
     9001 ccgcatcccc aggaaaaatc caatcgtcag aatggcgagt ccgctcgtca aacaggcata
     9061 cccattttca gaatttcaag aacgtgcata ccgaagcata gctgacaggg aagaaacttt
     9121 cccgaaaaac ataggtgcgg tgaggcttca ctcttcgcga aggatgcgca tgagctcact
     9181 cgcggcatgc gggaggcgtg cgccccgccg cgaaacgaga tagacgtgac ggggcatgtt
     9241 caattgccgc acttttactt ccttcatcca gccgtgttcg atttcccatt gcacgcacat
     9301 ccgcggcacg atggccacgc ccatgcccat ttgcacgaag cgcttgatgc tttcgatggt
     9361 gggcatttcg atgggcatat tgagcgcggt gcggtttctc gcaaacagct caatgacgcg
     9421 gcggcgaaag ggtgattcga cgctgtgcgc gatgaagatt tcttctccga gctcggcgac
     9481 atcgacttca cgcttcttcg cgaggatatg cttcggcggc acgaccagcg tcagctcgtc
     9541 tttcaggatt tccgtggcct ggagctgggc atcgcggggc acgaaagaga tcgcgccgag
     9601 atccaggcgg tagttcagga cttcatgagg gatgtcccgc gaaaacatgc ggtgcacgcg
     9661 tacctggacg ccggggtgct tttcacgaaa tttggccaag gcgggaagga gagcgtggat
     9721 ggagctctcg ttcactccca gagagagttc gccgcgcttc aagccttcca attcggagag
     9781 cccttttttc acttcatcgc gaaggttgat caggcgctcg gcatagtcct tcagaagtgt
     9841 tcccgcatcg gtgaggcgga caggccgcgc gccacggacg aagagaggct ggcccagcga
     9901 gtcctccagt ttgcggatgg ccaggctaat ggcgggctgg gtgcggtaaa ggcgttcccc
     9961 cgcgcgggag aagctgccct ctttcgcgac catcagaaat acctgcaatt catggagatc
    10021 caaggagccc tccccgacag tcagtataag acgaaattat caaaagtaga ataaagataa
    10081 attggaccgc agggtgcctc ccgtgcgtta ctagagcatg ggggacccta tggacgtcgt
    10141 acgcatcttt gacaccacgc tccgtgacgg cgaacagtcg ccgggcttct cgatgaacac
    10201 cgaggagaag attcgcctgg cgcggcagct tgcggccctg aacgtggacg tgattgaagc
    10261 gggtttcccc attgcgtcaa agggtgacct ggaagcggtt aagaaagtgg cgcaagaagt
    10321 tcggaccgtg ccgattgcgg cactcgcgcg cgccaaaaag gaagatgtaa gcgccgccat
    10381 tgaggctctg aaatcggcgg ccgcaccacg cctgcatatc tttctcgcta cttccgatct
    10441 gcaccttaac gtgaagctga agatgacccg cgagcaggcg ctcgaagcca tcagctcgat
    10501 gattcgctac ggccggcagc atgtggccga agtggaattc tccgccgaag atgccgggcg
    10561 aaccgacatc gattttctgt gccaggtatg ccatgtggcg gtggatgcgg gcgcgacggt
    10621 cctgaacctg ccggacacgg tgggatacgc ggtgccggaa gagtacggag cgatgtttac
    10681 caaggtccgt gaatacctgg gggatg
//
LOCUS       DASYVO010000341         7037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_189846, whole genome shotgun sequence.
ACCESSION   DASYVO010000341 DASYVO010000000
VERSION     DASYVO010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7037
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189846"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VGR03_11580"
     CDS             <1..283
                     /locus_tag="VGR03_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEV1994963.1"
                     /translation="EAFNMLEAGIATPEDIDKALKLGLNHPMGPFELVDLVGLDVRLK
                     ILEYLHQTLGEKYRPNALLRQYVQEGRLGRKSGRGVYDYTQQKAAGKKA"
     gene            314..871
                     /locus_tag="VGR03_11585"
     CDS             314..871
                     /locus_tag="VGR03_11585"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEV1994964.1"
                     /translation="MKSLAEKIFIQQGDLTEMDTDAIVNAANNDLILGAGVAGAIRRK
                     GGEEIQRECDAIGSIPVGYAAITTGGKLKARFVIHAASMQLGGKTNADSLRHAAAHSL
                     RIANERGLKTIAFPAVGTGIAGFPLKDCAEIMLRETAKHLRGESSLETVYFVLFDEAA
                     QGAFERAWRKIQAEPATDSAGAKGA"
     gene            868..1983
                     /locus_tag="VGR03_11590"
     CDS             868..1983
                     /locus_tag="VGR03_11590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV1994965.1"
                     /translation="MTPLAELLADRIRRCGPLTFADYMRECLYHPVHGYYSKTESKRF
                     ADYYTSVDVHPIFARLLARQFAEMWESLGRPAEFALVEGGAGVGRFAAQVLDFCEPKL
                     PAFYGALRYVAVERSASRREQATIQARRHADAGHFTESAEIPAHLAAGCFFSNELVDA
                     LPVHRVVMDGSTLKEILVGFSDGHFIDVLGPLSTCAISEYFAAQNIALCEGQHAEAGL
                     EACDWIGEIGRRLGRGYVLAIDYGHPASDLFDEHHMRGTLLAYHNHRASEDYHASPGE
                     QDLTAHVNFTALESWGRRSGLETAGFTSQTAFLLALGQGNEFADLYDEGQSESERTKA
                     RLQLKTLIYPEGMGERFQVLIQRKGVAPAPLTGLAGI"
     gene            complement(2594..3313)
                     /locus_tag="VGR03_11595"
     CDS             complement(2594..3313)
                     /locus_tag="VGR03_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1994966.1"
                     /translation="MRHDSVEVVAHLSPEEVEEGKLNDVYETIAQLQRRGTCTTEIIQ
                     RYQSEVIPLAEKSTFNVPKNKFLFLANRDIGNCYMAQRRYEDAEQSFKKIMEYLPIWP
                     GTDDSDYPINFRQIATAQMGQQRWEAAEESLKKSVALFDPQIERALKSDSEFSRTEHA
                     GNLIGSKARSLSYLAIVDLREGRTTEALKAAELAYEEVTRPHVQATFLNEVVKVGNSI
                     AQESGDKGAIAKWSQRSPRQN"
     gene            complement(3552..5705)
                     /locus_tag="VGR03_11600"
     CDS             complement(3552..5705)
                     /locus_tag="VGR03_11600"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEV1994967.1"
                     /translation="MALRRRVSGAFFLLTLIFLSCAAAPLPAQVPRPANFLAEHYDVS
                     ASLDAIGQSISATAKIDFKAVEASSSVRVELHPNLIVKEVRGADGKPLTFERDNQNPL
                     VVIVQLPTPVATAGHVTLTYTYAGLLVNEENSPVPGVRAALINKEGAYLLLPARWFPL
                     TNFPSNRYTATFRLNVPDIFAVAGTGKASAPSPMPGKNAVEGGRLLYTFECKNLAAHG
                     TFVAGNLQLNPKQAEGVNVAVYAPRASSANAADFAASVARSAIIFSDMFGPIPDPTFT
                     LILLPDGTLREYAAPGVLLLSRRIWDPKSSDRTIAGLVASQWWGIQVLPATPSDVWIS
                     DGLARYSEALYAEQNAGKEAGLRAVDEFAVGALMYEDAAPVAQAARLAPYSPDYRSVV
                     LNKGAMLFHMLRAQMGDVAFKSTLRDFYFQFAERSARIEDFENIAERRAQAAVKPPQE
                     PPNLRGFFAQWLNSTGVPEFSLEYVVYRTPKGFRVVGKIKQPLDTFRMPVDLRIDTEG
                     NPEQKTVDVIGTESGFTVETFGRPKPGGIKVDPNNVILKGSTSLRARAAIARGEDLAE
                     QGRFYDAVTQYQRALSIQPNRPLADFRMGEAFFYQKNYQAAANAFREALQTVPEPSEK
                     WTEVWSHIYLGKIFDMLAQRERAVNEYSKAKQTNDDTGGAQGTAEGFLKKAYSEGGAS
                     VAAPAAGTPAKPAVAIPPPASGEKPVLKKPNPPQQ"
     gene            complement(5767..6933)
                     /gene="lpxB"
                     /locus_tag="VGR03_11605"
     CDS             complement(5767..6933)
                     /gene="lpxB"
                     /locus_tag="VGR03_11605"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEV1994968.1"
                     /translation="MPPIPLLLSAGEASGDMYAARLATALKQRLDVAIFGMGGPQMRA
                     AGVEIITDYSEVSVVGITEVLKRLPSLFRAMRRLVDEAERRHPPLAILTDFPGFHLRL
                     ARKLRPKGIRNVYYICPQFWAWRPWRVNLVRRRFAQALCIFHFEEKFYADAGVPVKFI
                     GHPLVGNVKATLTREFFCKKYGLEERSKILTILPGSRRGEIAHHLPVLVEALAEIRQR
                     LPDPPAIVVAVAPGLEAPRLKAKFPADWHVRFIEDDTYNALAAADLAIVSSGTATVET
                     ALLGKPMIVVYRLSPLTARLAKPLVRTEFFSMVNLIAGRAVVPELIQHGFTPQRVAAE
                     AEKLLSASEEAKNRVEKMIRGLEEVRRLLGPPGAVERAADEIAKLLNAAQSNAN"
BASE COUNT         1440 a         1971 c         2124 g         1502 t
ORIGIN      
        1 cgaagcgttc aacatgctcg aagcgggaat tgccaccccc gaagacatcg ataaagcgct
       61 gaaactcggg ctgaatcacc ccatgggccc cttcgagttg gtagacctcg tcggtctcga
      121 cgtgcggctc aagattctcg agtatctcca tcaaaccctt ggggaaaagt accggcccaa
      181 tgccttgttg cggcaatacg ttcaggaagg acggctgggc cgcaagagcg ggcgcggcgt
      241 ttacgattac acgcaacaaa aagccgcggg aaaaaaagca tgatcgcatc agcgagaacg
      301 gattgattcc acgatgaaat cactcgcaga gaagatcttc attcagcagg gcgacctgac
      361 ggagatggat acggacgcca tcgtcaacgc ggccaacaac gatctaattt tgggcgcggg
      421 cgttgcgggc gccattcgcc gcaaaggcgg agaagagatc cagcgcgaat gcgatgcaat
      481 cggcagcatc ccggttggct atgcggccat cacaacgggc ggaaaactga aggcgcgatt
      541 cgtgattcac gcggccagca tgcagctcgg tgggaaaacg aatgcggact cactgcggca
      601 tgccgcggcg cattctttga ggatagccaa cgagcgcggc ctgaaaacaa tcgcatttcc
      661 agcagttggc accggcatcg ccggatttcc tttgaaagac tgcgcggaga ttatgctccg
      721 tgagacggca aagcatttaa gaggcgaaag ttctctcgaa acggtttatt tcgtactttt
      781 cgacgaagcc gcgcagggag cgttcgagcg agcgtggaga aagatccagg ctgaacccgc
      841 taccgattcg gcgggagcca agggcgcgtg acgccgctcg cggaattgct tgccgaccgc
      901 attcgccgct gcggccctct cacgttcgcg gactacatgc gcgaatgcct ctaccatccg
      961 gtccatggtt attacagcaa aacggagtcc aagcgtttcg cggactacta cacgagcgtg
     1021 gacgtgcatc ctatctttgc gcgtctcctg gcgcgacagt tcgcagagat gtgggaaagc
     1081 ctgggccggc cggcggaatt tgcgcttgtt gaggggggcg cgggtgttgg gcggttcgcg
     1141 gcgcaggttc tcgatttttg cgagccaaag ctcccggctt tttatggggc cctgcgctac
     1201 gtcgccgtag agcgctccgc ttcgcggcgt gaacaggcaa ccattcaagc cagacgccac
     1261 gcggacgccg ggcatttcac agaatcggca gagattccgg cacacctcgc agcaggctgc
     1321 ttcttttcca acgaactggt ggatgcgctg cccgtgcatc gagtggtgat ggatggaagc
     1381 acgttgaaag agattctcgt cgggtttagt gacgggcact ttatagacgt gttgggaccg
     1441 ctatcgactt gcgccatctc tgagtatttt gcggcgcaaa acattgcttt gtgcgagggc
     1501 cagcacgccg aggcaggact ggaagcgtgc gactggattg gtgagatcgg ccgccgcctc
     1561 ggacgaggtt acgtgctcgc catcgactac ggacatcctg cttccgatct ttttgacgaa
     1621 caccacatgc gtggcacgct tcttgcgtat cataatcatc gcgccagcga agattatcat
     1681 gcttcgcccg gcgagcagga cttaacggcg cacgtcaatt tcacggcgct cgaatcgtgg
     1741 ggaagacgat cgggcctgga gaccgcgggc ttcacttcac agacggcttt tctgctggcg
     1801 ctggggcagg gaaacgagtt tgccgacctc tacgatgaag gacaatccga gagcgagcgg
     1861 acgaaggcgc ggctgcaact caagacgctg atctaccctg aaggcatggg ggagcgattc
     1921 caggtgctta tccagcgaaa aggtgtggcg cccgcgccat tgacaggtct tgcgggaatc
     1981 taacggctga tttggtgtcc tactcaaatg caggagggtc cccttccgac atggtcggaa
     2041 tcttcattcg tcgcgctgcg ctcaagacag gttgcatcaa gggcatctgg atacccccct
     2101 gggttttttg taagtgttgc atctaaagaa cttaggcgat gcgtaagtcc tttagatgca
     2161 acacatacgc tgcgacttgt aaatgttgca tctaaagggc ttaggagttt acataactat
     2221 cgaagcggag ctgcttttct tgcgatgagc gtgggctgcg gatgctttgc tggggtgcgg
     2281 gtttgacgac ggaaaagtgg caaccagctg ccgcactcca aagtgtccgc accgggcagc
     2341 cgcgaacggg gcggattacc gtgagaactg cgagcttgat ttgcgatgac ggaaataaaa
     2401 aaggcggcag agaaaccctt cgaggctcag ggtaaactgc cgcaacctga aagatccgca
     2461 ctcttaccca ggaagtagcg taccaaatgg tgcatagcga gtcaaggagt ctttaggtgg
     2521 tggataagtt tccgatttgt gggtcaatga ggactgactt gaatttgacc gagtcgctct
     2581 caaaaaggcc gcgctaattt tggcgcggac tgcgttgtga ccactttgcg atggcgccct
     2641 tgtccccgct ctcctgtgca atcgagttcc ccacctttac gacttcgttt aagaacgtgg
     2701 cctgcacatg cggccgggtc acctcttcgt aggccagttc cgcggctttc aaggcttcgg
     2761 tggtgcgacc ttcgcgcaag tccacaatgg ccaagtaaga gagacttctg gccttcgacc
     2821 caatcaggtt cccggcatgt tccgttcggc taaattcgct gtccgacttg agtgctctct
     2881 caatttgggg gtcgaaaagc gccacggatt tcttcagcga ctcctccgca gcttcccagc
     2941 gttgctgtcc catttgcgct gttgctattt gccgaaaatt gattggatag tccgaatcat
     3001 ccgtccccgg ccagatgggc aaatactcca taatcttctt gaagctttgc tcagcgtcct
     3061 catagcgccg ctgcgccatg tagcaattgc caatgtcgcg gttggcgagg aagagaaact
     3121 tgtttttggg aacattgaag gtggatttct cggcgagcgg aatcacttcg gactggtaac
     3181 gctggattat ttctgtcgtg caagtgcctc ttcgctggag ttgagctatt gtttcgtata
     3241 catcgttgag ctttccttct tcgacctcct cgggggagag atgcgctaca acctcgactg
     3301 aatcgtgcct caccggcttt ggcgaggcgg gcgattgggt ttgcggagaa ggattttgcg
     3361 ctgaggcggg cgcgtggaca agaagcagtc cgaagagaaa gagcgtagga gcgcgagaga
     3421 aggacttagc gcgtgcgtag aggtgcatag gcggcgagac tataacatgg ggtgcaagtc
     3481 ctgtgtagtc ggatatcgga aactgactca gtgctagttt tgggagactc gctgttcctg
     3541 caatgagatt tctattgctg agggggattg ggttttttca agacgggctt ttcgccggag
     3601 gcgggcgggg gaatggctac ggcgggcttc gctggtgtgc cagctgcggg ggcggcgacg
     3661 gaggctccgc cttcggagta ggcctttttc aggaaacctt cggcggttcc ttgggcgccg
     3721 ccggtatcgt cgttggtttg cttggctttg ctgtattcgt tgacggcgcg ctcgcgttgc
     3781 gccagcatgt caaaaatttt tccgaggtag atgtgactcc agacttccgt ccatttttcg
     3841 gacggctccg gaacggtctg caacgcttca cggaacgcat tcgcggctgc ctgatagttt
     3901 ttctggtaga agaacgcctc gcccatccgg aaatctgcca gcggccggtt cggctgaatc
     3961 gaaagcgcgc gctgatactg cgtcacggca tcgtaaaacc ggccttgttc ggcgagatct
     4021 tcgccgcgcg caatggccgc gcgggcacgc agcgaagtgc ttcccttcag aatgacattg
     4081 ttggggtcga ccttgattcc acccggcttc ggccgtccaa acgtctctac cgtgaatccc
     4141 gattccgtgc cgatcacgtc gacggttttc tgctccgggt ttccttccgt atcgatgcgc
     4201 aaatccaccg gcatgcgaaa ggtgtccaga ggctgcttga tcttgccaac gacgcgaaat
     4261 cccttgggcg tgcggtatac gacgtattcc agcgagaact ccggcacacc cgttgaattc
     4321 agccactggg cgaaaaagcc gcggaggttc ggaggctctt gcggtggctt cactgcggcc
     4381 tgtgcgcggc gctccgcgat gttttcaaaa tcctcgatgc gcgcgctcct ttccgcaaat
     4441 tgaaagtaga aatcgcgcaa ggtggacttg aacgccacgt cgcccatttg cgcccgcaac
     4501 atgtgaaaga gcatcgcacc tttgttcagc accaccgaac ggtagtcggg ggagtaagga
     4561 gccagtcgtg ccgcttgcgc gacaggtgcg gcatcctcat acatgagcgc gccaaccgcg
     4621 aattcgtcca ccgcgcggag tcccgcttct ttgcccgcat tttgctccgc gtagagcgct
     4681 tcagaatatc gcgccagtcc atcgctgatc cagacgtcgc tgggcgttgc aggtagaacc
     4741 tgaattcccc accattgcga ggccaccagg cctgcaatcg tcctgtcgct gctcttgggg
     4801 tcccagatgc gccggctgag aagcaaaacg ccgggagcgg cgtactcgcg cagggtgccg
     4861 tcgggcagca ggatcaacgt gaaagtagga tccggtatgg gtccaaacat gtctgagaaa
     4921 ataatcgccg agcgcgccac gctcgccgca aaatccgccg cattcgcgga cgaggcccgc
     4981 ggagcgtaga ccgcgacgtt gacgccttcc gcctgctttg gattgagctg gagattgccc
     5041 gcgacaaacg tgccatgcgc cgcgagattc ttgcactcga aagtgtagag aagccgcccg
     5101 ccttccacgg cgttcttgcc gggcatgggt gacggagcgg aggctttgcc ggtcccggcg
     5161 acggcaaaga tgtctggaac gttcagacgg aatgtcgccg tatagcggtt cgacggaaaa
     5221 tttgtgagcg gaaaccacct tgccgggagc aataggtaag cgccctcttt gttgatcagc
     5281 gcggcacgca cacccggtac ggggctgttc tcttcattta cgagcagacc cgcataagtg
     5341 taggtgaggg tgacgtggcc ggcggtggcc acgggtgtgg gcaactggac gatcacgacg
     5401 agtgggttct gattgtcgcg ctcgaaggtc agcggtttgc cgtcggctcc tcttacttct
     5461 ttcacgatca ggttcgggtg cagctcgacg cgcacactgc tggaggcttc cacggctttg
     5521 aaatcgattt ttgcggtcgc ggaaatcgat tgtccaatgg cgtccagcga cgcggacacg
     5581 tcgtagtgtt cggcgagaaa gttcgcgggc cggggaacct gcgccgggag cggcgcggca
     5641 gcacaagaca gaaagatcag cgttaaaagg aaaaatgcac cagagactct tcggcgaagg
     5701 gccatcggac ccctttcaaa tatcttcaat tccgaaccta tgtacaccta taggatagca
     5761 aactcatcag ttcgcgttgc tttgggccgc gttgagcagc ttggcaatct catccgccgc
     5821 cctttcgacg gctcccgggg ggcccaggag ccttcgcact tcttctaacc cgcggatcat
     5881 cttttccact cgattcttgg cttcttctga ggcagagagc agcttttccg cttccgcggc
     5941 gacccgctgc ggcgtgaagc cgtgctggat caattcaggg acgacggcgc gcccggcgat
     6001 gagatttacc atgctgaaaa actcggttct aaccagcggt tttgccagcc gcgccgtcag
     6061 cggagagagc cggtaaacga caatcatcgg tttaccgagc agcgccgttt ccacagtggc
     6121 cgtgccactg gagacgatcg ccaggtccgc agcggccagc gcattgtagg tgtcatcctc
     6181 gataaatcgc acatgccagt cggcagggaa tttcgccttt agccgcgggg cttccagccc
     6241 cggcgccacg gcaaccacaa tcgcaggcgg gtctggtagc ctttggcgga tttccgcgag
     6301 agcttctacc agcaccggta aatggtgcgc gatttctccc cgccggcttc ccggcaaaat
     6361 cgtcagtatc ttgctccgtt cttccagacc gtattttttg cagaaaaatt cccgcgtcag
     6421 tgtagctttc acgtttccca cgagcggatg gccgatgaat ttcaccggca caccggcatc
     6481 ggcgtaaaac ttttcttcga aatgaaaaat acacagtgcc tgcgcgaacc ggcgccgcac
     6541 caaattcacg cgccacggcc gccacgccca gaattgcggg caaatgtaat agacgtttcg
     6601 aatgcccttg gggcgcagct tgcgcgccag ccgcagatga aaaccgggga agtccgtcaa
     6661 aatcgcaagc ggcgggtgcc ggcgctccgc ttcgtccacg agccgcctca tcgcacggaa
     6721 caacgacggc aaccgcttca acacttcggt gattcccacc acggagactt cggaataatc
     6781 cgtgatgatc tcgacgcccg ccgcgcgcat ctgcggcccg cccatcccga agatcgccac
     6841 gtccaggcgc tgcttcagcg ccgtcgcgag ccgcgcggcg tacatgtctc cggaagcttc
     6901 gccggcggag agaaggagcg gtatgggtgg catgtgtgag tggcgcagga atcagtcgct
     6961 ggccggcgac accgagggtt cagccgggcg attggactcg atcggaaggt gcagctcctg
     7021 ctccttgtat tgcagct
//
LOCUS       DASYVO010000342         1513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_135400, whole genome shotgun sequence.
ACCESSION   DASYVO010000342 DASYVO010000000
VERSION     DASYVO010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135400"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1303
                     /locus_tag="VGR03_11610"
     CDS             212..1303
                     /locus_tag="VGR03_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1994969.1"
                     /translation="MRGDDQQQSGVFSYISAEQRVPQNHPLRSVRKMVDEVLAKLSPR
                     FAKMYAKRGRPSIAPEKLLRALLLQVLYTIRSERQLMEQLDYNMLYRWFVGLNMDDPI
                     WDVTVFTKNRERLLKGDIAQAFFQQVLEQARAQSLLSEEHFTVDGTLIEAWAGQKSFQ
                     RKDQPSPPPQDGGSNPTVNFHGEKRSNQTHQSTTDPEAMLYRKSGGSESKLSYLGHVL
                     MENRNGLVVDTRLTRATGTAERDAALDMAARIPGGTRRVTLGGDKNYDTRDCVSKLRE
                     AAVTPHVAQNQHERRTSAIDERTTHHEGYAISQRKRKRVEEIFGWIKTVAGLRKTRHR
                     GKDRVGWMFTFAAAAYNLVRMRNLAQAIA"
BASE COUNT          369 a          406 c          476 g          262 t
ORIGIN      
        1 tctgcgccgt tagcgcaggc gcaagcaggc tcgctagaag ggctccctcg aacatgcgca
       61 tgataacttc tctcataaag tttagggcag accgatgcag cctgccctaa agatacaata
      121 gctatgactc ttgtcgagaa gccctctagc aggatgcgga aaaaagcaga ttccgatgtg
      181 acgcaggaag cgtgagcgtc gtcttgtata catgcgaggc gacgatcaac aacagagtgg
      241 cgtattcagc tacatttcag cagaacagcg tgttccccag aatcatccgc tgcgatccgt
      301 gaggaagatg gtggacgagg tgttggcgaa gttgtcgccg cgtttcgcca agatgtacgc
      361 gaagcggggc cggccgtcga tagctcccga gaaactgctg cgggcgctac tgctgcaggt
      421 gctctacacg atccgtagcg agcggcagtt gatggagcag ctggactaca acatgctgta
      481 ccggtggttc gtgggattga acatggacga ccccatctgg gacgtgacgg tattcaccaa
      541 gaaccgtgag cggctgctga agggagatat cgcgcaggcg ttctttcaac aggtgctgga
      601 acaggcgcgg gcgcagagct tgctgtcgga ggaacacttc accgtggacg gaaccttgat
      661 cgaagcctgg gcgggacaga agagctttca gcgaaaggac caaccgtctc caccgccgca
      721 agacggcggc agcaatccga cggtgaactt tcacggcgag aaacgcagca atcagacgca
      781 ccaatcaacg accgatccgg aagcgatgct gtaccggaag tcgggtggga gcgaatctaa
      841 actgagttac ctggggcatg tgttgatgga gaaccgcaac gggttggtgg tggacacgcg
      901 cctgaccagg gccacgggaa cggccgagcg cgacgcggcc ttggacatgg cggcgcggat
      961 tcctggcggc acccggcgcg tgacgttggg cggcgacaag aactacgata cgcgggattg
     1021 cgtgagcaaa ttacgggagg cggcggtgac accacatgtg gcacagaacc aacatgagcg
     1081 gcgcacgagc gcgatcgatg aacgcacgac gcatcacgag ggctacgcga tcagccaaag
     1141 gaaacgcaag cgcgtcgaag agattttcgg ttggatcaaa accgtagcgg ggctgcgtaa
     1201 gacgcggcat cgcggcaagg acagggtcgg gtggatgttc acctttgcgg cagcggccta
     1261 caatctggtt cggatgcgga atctagcgca agccatcgcc tgaatctccc ccaaggcgcc
     1321 cgacccagca ctcgggcacc tcaaccggcc acgaaatcac tcacaaaata acaacgaggg
     1381 caaggagatc aagccgcctg cttggttttt ccgcatcctg ctagaacgca cgccgccata
     1441 ggtacatttt gcgaaattac ctgcggtaaa tgcccgaatt gcctatatta tggaatggaa
     1501 gcggcacttc tgg
//
LOCUS       DASYVO010000343         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_178210, whole genome shotgun sequence.
ACCESSION   DASYVO010000343 DASYVO010000000
VERSION     DASYVO010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178210"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2151
                     /locus_tag="VGR03_11615"
     CDS             <1..>2151
                     /locus_tag="VGR03_11615"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEV1994970.1"
                     /translation="KPGSPEAAEPISGSVRWDGKILGPSGGPTFQGHVRGERARYDGV
                     FLDFLDGDLTYSPTELSLARGHARRGEMGTDIEGTLSLTSWNFLPQNQWSADVNFEKV
                     PVEGIEQLIGWSHPVSGLLTGQFHGHGTREEPSVTGLFDLADGKIYGLTFNRLRGQLN
                     LLPDEVRIANAELRFFPLGKENGRGAGIITGSAGYRYADQTITAELVGAALPLENFEK
                     LQSVRLPVGGQVSFKLKASGPVRSPLGDGTFRVVDLRVGQEVIGSFDGSLTSDGHMAH
                     LELGSAMSTGEISGGLTLGLADPYPLGGKISIKNIDLDPFLLTALHLGKFGGHANADG
                     DISLNGSLKRPEGIVADASFSRLALTYVNVRLENVGPVHFRSSKESLEIDPATLRGTD
                     TNLQIGGSVRFAGRRTVGLRLNGALDLRLISGFVPGVDAHGPAQINASIEGTLDRPRI
                     TGRVHIENASARAADLPTGLSAINGDLIFDTTRLYFENVSAEAGGGTLHLSGSISYAE
                     SPLRFDVSLRSDRVRIRYPEGMSWLVGGSLRLSGTPAGGILSGRVMIERVTLNQGLEV
                     AGMLVSAKEGITGPSTSSPFLRNLQFDVEAVSTPDARMEWPGAELQAEANLRARGTWE
                     HPILLGHIHILSGELYFAGNRYRVSRGDLNFANPFRLDPVLNVEATTTIQQYEITLNF
                     NGPASKLTLAYRSDPPLPANDIITLLALGQTSSEA"
BASE COUNT          435 a          625 c          657 g          434 t
ORIGIN      
        1 gaagcccgga tcacccgagg cagcagaacc gatctcgggc agtgtacgct gggatggcaa
       61 gatcctcggc ccatccggag gccccacgtt tcaagggcac gtgcgcgggg agcgggcgcg
      121 ctacgacggg gtgttcctgg atttcctcga tggggatctc acctattctc caaccgagtt
      181 gtccctggca cgcggacatg cccggcgcgg cgagatggga acggatattg aaggtaccct
      241 ttccttgacc agttggaatt ttcttccgca gaatcaatgg tcggcggatg tcaattttga
      301 aaaagttccg gtcgaaggca tcgaacaact catcgggtgg tcgcatcccg taagcggctt
      361 actgacgggt caatttcacg gacatgggac acgcgaggag cccagcgtca cgggtctttt
      421 cgacctcgcg gacgggaaaa tctacgggct gaccttcaat cgtctccgcg ggcagctgaa
      481 tcttctgcct gatgaagtgc gcattgccaa tgcagagctg cgattctttc cgctgggaaa
      541 agaaaatggc cgtggggccg gaatcatcac gggctcggcg ggctaccgct atgcagatca
      601 gacgataacc gcggaactcg tcggtgcagc gctacctctc gagaatttcg aaaagctaca
      661 gtcggtgcgc ctcccggtgg gaggacaagt cagtttcaaa ctcaaggcca gtggtccggt
      721 caggtcacca ctcggcgatg ggaccttccg cgtcgtggat ctccgagtcg gccaggaagt
      781 catcggtagt ttcgatggca gcctgacctc cgacgggcat atggcgcatc tcgaactggg
      841 ctcggccatg agtacgggag aaatttccgg cggcctcacg ctcggacttg ccgatcccta
      901 tccattaggc gggaaaatct cgatcaagaa cattgacttg gatcctttcc tgctaactgc
      961 cttgcacctg ggaaaattcg ggggccatgc gaatgccgat ggcgatattt cgctgaatgg
     1021 ctccttgaaa cggccggaag gcattgttgc ggacgccagc ttctcgcgcc tcgcgctaac
     1081 ttatgtaaat gtccggctgg agaacgtagg gcccgtccat ttccgatcgt cgaaagagag
     1141 cctggagatc gatccggcga cactgcgagg aacggacacc aatcttcaaa tcgggggttc
     1201 ggtgcggttt gccgggcgcc gcacggtcgg cttgcgcctc aacggcgcgt tggatctgcg
     1261 cctcatcagc ggattcgtgc caggtgtcga tgcccacggg ccagcacaaa tcaatgcttc
     1321 catcgaaggc acgctggacc ggccgcgcat cacgggccga gtacacattg agaatgcttc
     1381 cgctcgtgcg gcggatctcc cgacaggctt aagcgcaata aacggcgacc tgatattcga
     1441 tacaacgcgt ctgtatttcg aaaatgtaag cgccgaagcg ggtggcggca cgctgcatct
     1501 ttcgggcagc ataagttacg cggagagtcc gctgcgcttc gacgtcagct tgcgctcgga
     1561 tcgcgtacgc attcgctatc ccgagggcat gagctggcta gtgggaggct cgctgcggct
     1621 gagcggcacg cccgcaggag gcattctttc cggccgcgtg atgatcgaac gcgtgaccct
     1681 caatcagggc ctggaagtcg ccgggatgct ggtctccgcg aaagaaggca taaccgggcc
     1741 gtctaccagc tcgccgttcc tgcgcaatct gcaattcgac gtcgaagcgg tatcgacgcc
     1801 tgatgcgcgc atggagtggc cgggcgctga gcttcaggcc gaagccaatt tgcgcgcgcg
     1861 cggaacttgg gagcatccca ttcttctcgg gcacatccac attctctcgg gtgaattgta
     1921 tttcgcggga aatcgttacc gcgtttcgcg gggggacctg aacttcgcga atccgttccg
     1981 gctcgatccg gttctgaacg tcgaagcgac gacgacgatc cagcagtatg aaatcacgct
     2041 gaatttcaat ggaccggcca gcaaactcac gctggcgtat cgctccgatc cgcctctgcc
     2101 cgcgaacgac atcatcacgc tgttggcgct gggccaaacc agctccgagg c
//
LOCUS       DASYVO010000344         2749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_63768, whole genome shotgun sequence.
ACCESSION   DASYVO010000344 DASYVO010000000
VERSION     DASYVO010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63768"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..601
                     /locus_tag="VGR03_11620"
     CDS             149..601
                     /locus_tag="VGR03_11620"
                     /inference="COORDINATES: protein motif:HMM:NF039194.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HEV1994971.1"
                     /translation="MRGRPFQPGNQYGRGRPPGSRNKIGSVVQEALESHAETLIKKCV
                     MLALQGNLTAMKLCMERLVPAPRQRVIKFKLPAIKTITDLTVASEAVVRGATRGQLTP
                     SEGQSFTAMLDDRRRMMESKELDSRVRALEEGRNEQGPEDEDGKKGSS"
     gene            610..885
                     /locus_tag="VGR03_11625"
     CDS             610..885
                     /locus_tag="VGR03_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994972.1"
                     /translation="MQKTLEKRIRKLESMLQSRARSAVVFRYGRVKHLPEGAVGERHI
                     AIATSATTAVSHVEQCEFEERIGPAGCKDDFGFRVYLYSEADDGGQP"
     gene            869..2080
                     /locus_tag="VGR03_11630"
     CDS             869..2080
                     /locus_tag="VGR03_11630"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HEV1994973.1"
                     /translation="MAANLKEVFQQSLYRTSNPAVVMWHRVKPGRTKQTMIVSLKPAQ
                     SSVFACDSRFRVLAAGRRFGKTYLGCTELCRAAWGKGRLAWYVAPTYKQGKRIAWNPL
                     KQMTRKLWASPPNETDLRIELINGGTIALRGADNYDALRGDGLDFVVLDEYASMAPAA
                     WTEVLRPALADKQGGALFIGTPRGHNHFFDLYEAAQNQRHWSAFQFTTEQGGNVTHEE
                     LESATHELDERTYRQEFQASFENLGAGRVYYAFDRQAQQGTVPYSASFPLWWSLDFNV
                     NPTCSLIGQMVISRNREHIRVLDELMLPESNTLAACEEFLSRTEKWKSGRPLDRLHLW
                     RRDGGTPRDVGVADGLANCQELLRPLHRSLSGAFLRAVQESAGEGPGQLRQRGAAEPC
                     RRAPPLDRFAL"
     gene            2261..2512
                     /locus_tag="VGR03_11635"
     CDS             2261..2512
                     /locus_tag="VGR03_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994974.1"
                     /translation="MTCPECGAQAEQLAYCDGCFLLCCYDCVPNVNADCQHPTDEWLV
                     RFKVSNSISGKPLNQPKFVLRRTNIAPSGHMWRTVGTGF"
BASE COUNT          615 a          766 c          838 g          530 t
ORIGIN      
        1 ccatgcccga gtgattgaga tcgagccgac ctacgtggac gtgattataa gacgctggca
       61 agagttcagc ggcaaatcgg cagtcttaca aggtaggact ttcgcggaag tggctgatga
      121 gcgtttaccg aaggcagcgt aggccagtat gcgcggcaga ccatttcaac ccggcaacca
      181 atacggtcgc ggccgcccgc cgggtagtcg aaataaaatc ggtagcgttg tccaagaagc
      241 gctggagagc catgccgaga cccttataaa gaaatgcgta atgttggccc tgcaaggcaa
      301 cctaacggcg atgaagttat gcatggagcg gctcgtgccg gcgccgcgac agcgcgtaat
      361 aaagttcaag ctgcccgcca tcaagaccat aacagacctg acggtggctt ccgaggccgt
      421 ggtgcgaggc gcaactcgcg gccagctcac gccaagcgaa gggcaatcgt tcacggcgat
      481 gcttgacgac aggcgtcgca tgatggagag caaagaattg gactcacgag tacgcgccct
      541 ggaggagggc cgtaacgaac agggcccgga agacgaagac ggcaagaagg gaagctcatg
      601 aggttcgtca tgcagaaaac attggagaag cgaatccgaa agctcgaatc gatgttgcag
      661 tcccgtgccc gttcggctgt agtgtttcgc tatggccgcg tgaagcatct tcccgagggc
      721 gccgttggcg agcggcatat agcgatcgcc accagcgcaa ccaccgcggt ctcccacgta
      781 gaacagtgcg agttcgagga gcggattggg ccggcaggct gcaaggacga tttcggcttc
      841 cgcgtgtacc tgtattcgga ggccgacgat ggcggccaac cttaaagaag tttttcagca
      901 atctctatat cgcacgtcaa atccggcggt agttatgtgg catcgagtca agccgggaag
      961 gaccaaacaa acaatgatcg tttcgttgaa accagcacaa tcttccgtat tcgcctgcga
     1021 cagccgattt cgcgtcctgg ccgcaggcag acgcttcgga aagacctatc tggggtgcac
     1081 tgagctgtgc cgggccgcct ggggcaaagg ccggcttgcc tggtacgtgg ctcccaccta
     1141 taaacaaggc aagcggatcg cgtggaaccc gttgaagcag atgacccgta aactttgggc
     1201 ttctccacca aacgaaaccg atttgcggat tgagctgatt aatggtggca cgattgcgct
     1261 tcgcggggcc gacaactacg atgcgttgcg tggcgatggc ctggacttcg tcgttctgga
     1321 tgaatatgct tccatggccc ccgccgcctg gaccgaggtg ctgcgtcccg cgctagccga
     1381 taaacagggc ggggctctgt tcatcggcac accgcgcggc cacaatcact tcttcgatct
     1441 ctatgaggcg gcccaaaatc agcgccactg gtctgccttc cagttcacca ccgaacaggg
     1501 cggcaatgtc acccacgagg aactggaaag cgctacccac gagttggacg agcgaaccta
     1561 tcggcaggag tttcaggcca gctttgaaaa tttgggcgcg gggcgtgtct attacgcttt
     1621 cgatcgccag gcgcagcaag gcacggtgcc ttacagcgcc tcttttccgc tgtggtggtc
     1681 actcgatttc aacgtcaatc ctacttgctc gctgattggg caaatggtca tttccaggaa
     1741 tcgcgagcac attcgcgttc ttgacgagct gatgctgccg gagtccaata cactggcggc
     1801 gtgcgaggag tttttgagcc gcaccgagaa gtggaagagc ggcaggccgc tcgaccgtct
     1861 ccatctatgg cgacgcgacg gcggaacacc gcgagacgtc ggcgtcgcgg acggattggc
     1921 aaattgtcaa gaacttcttc ggccgctaca ccgatcgcta tcaggcgcat ttctgcgtgc
     1981 cgtccaagaa tccgccggtg aaggaccggg tcaactgcgt caacgcggtg ctgctgaacc
     2041 atgccggcga gcgccgcctc ttgatcgatt cgcgctgtaa ggaactggca aaggatctcg
     2101 agcaggtggt ttggaaaacg gatccaagcg gcaacgcgct ttccgagctg aacaacaggg
     2161 atcgaatgcg tacacacttg tcggacgcgt tgggatatta cgtccatcgc gaatttccga
     2221 tgcgcggcgt gagcggcgag cgcggagggc cggcgatcct gtgacttgtc ccgagtgcgg
     2281 cgcccaggcg gagcagcttg cttactgcga tggctgcttc ctcctgtgct gctacgactg
     2341 cgtgccaaac gtaaacgccg attgccagca tccaaccgat gagtggctcg ttcgctttaa
     2401 ggtgagcaac tccatatcgg gaaagccgct caaccagccg aaattcgtgc tgaggcgcac
     2461 aaatatcgca ccttctggcc acatgtggcg aaccgtgggt accggttttt gaaatgatcg
     2521 ccaaatccta tgacttatcg cggcgatcag ggccgcaagc ttggtatttc tcggccaccg
     2581 cgattgttat cgccccgacg aggtcgcggt cgagcagaag cagctgaggg cgacccaaat
     2641 aggacgttta cgacggattt tggactaacg cacgcatcgt gaaccaattc ctcccgaagg
     2701 gatgcgcagc ggcgccaaga agagtaggcg gggaagcatc cgagacatg
//
LOCUS       DASYVO010000345         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_248601, whole genome shotgun sequence.
ACCESSION   DASYVO010000345 DASYVO010000000
VERSION     DASYVO010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248601"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VGR03_11640"
     CDS             <1..237
                     /locus_tag="VGR03_11640"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEV1994975.1"
                     /translation="IDELDLLHLRGDRHYYETHGPRDHIHVACLRCGKVREFESRLYE
                     QLKEQIARDFHMKVTVSRTEVGGYCAECLQKTKA"
     gene            complement(241..>2086)
                     /locus_tag="VGR03_11645"
     CDS             complement(241..>2086)
                     /locus_tag="VGR03_11645"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV1994976.1"
                     /translation="MPPAPLKITVLGSGTSMGVPTLGCPCRVCKSNDPHDKRLRPSLL
                     ISRGVQNVVIDTTPDFRQQALRVGLERLDAILLTHGHADHILGFDDIRPFNIRQRSAL
                     PVYSNEETFRIVRRVFAYVFDDKPTLSTVPSVTLNAIRSPFELAGIPFVPVPLLHGEM
                     EVLGFRFGRAAYLTDFSKIPDSSMALLEGLDELVLDALRDIPHPMHQTVEQALALIQQ
                     LKPRRAWFTHIAHDLPHAETNERLVKMGYPHVQLAYDGLEFEVQLDAADEVSRERGGS
                     HESLAAMGGARSTRLSAFSSSQAWASRYATYGHTSVLAIGNFDGIHLGHQAILRATVE
                     RGQTLNAVSTALTFDPSPRKVLRPESAPLRLSTNAQRMEWFNALGLEAVVVLPFTLDL
                     ARLSPAEFVEQILVRDLNVKAVLVGEDFRFGHKQAGDVKLLSELGTKHGFDVVIVPPV
                     VHRGEVVSSTIIRREVAEGDVSHAARLLGRPFALTGEVVSGTGTGRRFTFPTLNLTAE
                     QELLPARGVYVTRTCLDGETRSHRSVTNIGMRPTFNGSSLSVETHLLDSQLPATPKRM
                     EVRFWKRLREEKKFSGPEELRAQIASDIARANKFFSRLRHSRVIRQPA"
BASE COUNT          441 a          576 c          672 g          397 t
ORIGIN      
        1 attgacgagc tggatttgct gcatctgcgg ggcgaccggc actactacga aactcatgga
       61 ccgcgcgatc acattcacgt ggcgtgcctg cgctgcggca aggttcgcga attcgagagc
      121 cgcttgtacg aacaattgaa agaacaaatt gcgcgcgact tccacatgaa ggtgacggtt
      181 tcccgcacgg aagttggcgg ctactgcgcc gagtgcctgc aaaaaacaaa agcatgaggc
      241 tcaagcgggc tggcggatca cgcgggaatg gcgcaggcga gaaaagaatt tgttggcgcg
      301 ggcgatgtcg ctggcgatct gcgcgcgcaa ctcttcgggg ccgctgaatt ttttttcttc
      361 gcgaagacgc ttccaaaatc ggacttccat gcgtttcggt gtggccggca gctgactgtc
      421 cagcaggtgc gtttcgacgc tgagcgacga gccgttgaac gtcgggcgca tgccgatatt
      481 cgtaaccgag cgatggctgc gtgtttctcc gtcgagacac gtacgcgtaa cgtaaacgcc
      541 ccgagcgggg agcagttcct gttccgcggt gaggttcagg gtagggaagg tgaagcggcg
      601 ccccgtcccc gtgccgctga ccacttcacc ggtaagagcg aagggccggc ccagaagacg
      661 cgcggcgtgg gacacgtcgc cttcggcgac ttcccggcga atgatggtgc tggagaccac
      721 ttctccgcga tggaccaccg gaggaacgat cacgacgtcg aatccatgtt ttgtgccaag
      781 ttcggatagc aacttcacgt cgccagcttg cttgtggcca aaccgaaaat cttcgccaac
      841 cagaacggct ttcacattca gatcgcggac aagaatttgt tcgacaaatt cagcggggga
      901 gagccgcgcg agatcgagtg tgaacggcag gaccacaacg gcttcgaggc cgagcgcgtt
      961 gaaccactcc atacgctgcg cgttggtcga gagccgcagc ggcgcagact ccgggcgaag
     1021 cactttccgc ggcgagggat caaacgtgag cgccgttgag actgcattga gcgtttggcc
     1081 gcgctccacg gtggcgcgaa gaatcgcctg atggcccaaa tggatgccat cgaagttgcc
     1141 aatggccagc acgctggtat ggccataagt tgcatagcgc gacgcccagg cctgtgaaga
     1201 cgagaaggct gacaatctcg tggacctcgc gccccccatc gcggctagcg attcgtgcga
     1261 acccccgcgc tcgcgcgaaa cttcatcggc cgcgtccagc tgtacttcaa attcgagacc
     1321 atcgtaggcc aattgcacat gcgggtagcc catttttaca aggcgctcat tggtttcggc
     1381 gtgcggcagg tcgtgggcga tgtgcgtaaa ccacgcgcgt cgcggcttga gttgttgaat
     1441 gagtgcgagc gcttgttcca ccgtctggtg catgggatga ggaatatctc gcagagcgtc
     1501 cagcaccagt tcatcgagtc cctcaagcag cgccatcgaa gagtctggaa ttttgctgaa
     1561 gtcggtgaga tacgcggccc gcccaaatcg aaatcccagc acttccatct ccccgtggag
     1621 cagaggcacg ggaacaaacg ggatgccggc aagttcaaac gggctccgga tggcgtttag
     1681 cgttacgctc ggcacggtgc tgagcgtcgg tttgtcgtcg aaaacgtagg cgaacacgcg
     1741 ccggacgatg cgaaacgttt cttcgttgct gtagacgggt agcgccgacc gctgacggat
     1801 attgaagggg cgaatgtcat caaagccgag gatgtggtcg gcatgcccgt gcgtcagcaa
     1861 aatggcgtcg aggcgttcta gtccaacgcg gagggcctgc tgacggaagt ccggcgtggt
     1921 gtcaatcacc acattttgga cgccgcggga gatcagcagg gaagggcgca gccttttatc
     1981 gtgcggatcg ttggacttgc aaacgcggca agggcatccg agcgtgggaa cgcccatgga
     2041 cgtgccggag ccgagcacgg taatttttag cggagcagga ggcatg
//
LOCUS       DASYVO010000346        23536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_178270, whole genome shotgun sequence.
ACCESSION   DASYVO010000346 DASYVO010000000
VERSION     DASYVO010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 23536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23536
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178270"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VGR03_11650"
     CDS             <1..695
                     /locus_tag="VGR03_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEV1994977.1"
                     /translation="LLLDVTPLSLGIETYGGAVAKIIPRNSTIPASAQELYTTGVDNQ
                     TGIDIHVLQGERELAKDCRSLARFTLKVPPAPAGLPRIEVKFLIDANGILQVGAKDLR
                     TGEQHSLEVQPSYGINDSEIERMLEESIEYAEQDFAERQVIEARTESESILTATAKAL
                     ANPQSASLPSEERAKIDASVAALQDSVVGSDYKLIRKRIDDLNQTTEHLAELLMNSAI
                     STALEGRKLAEV"
     gene            777..1187
                     /locus_tag="VGR03_11655"
     CDS             777..1187
                     /locus_tag="VGR03_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEV1994978.1"
                     /translation="MGGSNPYIEEVKYKPATKKFKVTFLPSGKTIEVDPEKIPYGHNG
                     LPGSILDFSEGLKAGLDHACGGVCACSTCHVIVREGLESCNEATDAELDELDEAPGIT
                     PKSRLGCQTVPDGTTDIVVEIPEWNKNYAKELDH"
     gene            1272..1490
                     /gene="iscX"
                     /locus_tag="VGR03_11660"
     CDS             1272..1490
                     /gene="iscX"
                     /locus_tag="VGR03_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEV1994979.1"
                     /translation="MPPTFGWDNLEDIAIGLADKFPDTDPLTVRFTDMHKWITELSGF
                     SGDPATSNESKLEAIQMAWHEEFQDRQS"
     gene            1951..2025
                     /locus_tag="VGR03_11665"
     tRNA            1951..2025
                     /locus_tag="VGR03_11665"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1984..1986,aa:Glu,seq:ttc)
     gene            2149..2658
                     /locus_tag="VGR03_11670"
     CDS             2149..2658
                     /locus_tag="VGR03_11670"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEV1994980.1"
                     /translation="MSYAEKHLIAGETVQYETRLHWIVMLGHVLIAAVLVLIGVSLLL
                     TPWSSVKGGEASLAGALRWAGAVCLVVAAIFFGVGLVRRNATEMAVTNKRVIVKSGLA
                     NRRTIELLLPRIESIAVEEPALGRLLGYGTVIVRGTGGTPEVFSQIARPLEFREQVQR
                     QIAGEPKSS"
     gene            complement(2707..5046)
                     /locus_tag="VGR03_11675"
     CDS             complement(2707..5046)
                     /locus_tag="VGR03_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV1994981.1"
                     /translation="MFTRKTTRFIGIVALFFLCVMSTRAQRVRGELHLEVHDPHGAAV
                     SPAGELVSEANGFRRTFVAGPEGRYVAQDLPFGVYRLSLQAEGFAAWSDLIEIRSEVP
                     VRVAVTLGVAPVTTQVQVNDSATLVDPYATGTLYSIGRQTIEEHDAAQPGRELLDLVS
                     EQPGWVYEANGVLHPRGSEYDVQFVVDGLPLTQNRSPAFAPSFDAGEVESLRVLTASF
                     PAEYGRKLGGIVEVTTKKDVQAGWHGRLDAGGGSFSTQNGAAGISYARGANRFSVGGE
                     GFHTDRYLDPPVLANYTNRGNAAGFSASYERDFSTSDRLRVSVMHNVVRFLVPNELVQ
                     QQARQRQDIQNTETSGQIFFQHTISSDLFLSFSGSVRDSSATLTSNRFATPVIVSQDR
                     GYREVYLRADLAGHHGHHDWKTGVDSIFSSVHEALQYAITNPGAFLPGTLLTFPFPYQ
                     RKLDVEPSIYLQDQMRLGRWNVSAGLRFDHYGFVVHESAWSPRIGVSRFVPALNLLLH
                     ASYDRVFQTPAIENLLLASSLLLDAISPSVLRLPVRPGRGNFYEGGVTKAFLGKLRLD
                     ANVFRRDFRQYSDDDVLLDTGVSFPIAFARARIFGEEIRLEVPQWRRFSGYLSYANQS
                     GYGQGPITGGLFLGSDAANGLTDTSKFAVTQDQRNSLRARVRFQAPRHAWLAMGAQYG
                     SGLPADVGNAKVSDLLAAFGQQILDRVDLARGRVRPNFSVDLAAGAEIYHKEQKSAAL
                     QIQVANLANRLNVINFASLFSGAAVATPRSVSARMKFTF"
     gene            5282..5917
                     /locus_tag="VGR03_11680"
     CDS             5282..5917
                     /locus_tag="VGR03_11680"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEV1994982.1"
                     /translation="MTPRNVTTGTPFTIGHSNHDLGDFLAVLAKYDVKTLCDVRSRPG
                     SFRFPQFNREPLEALLTSAGLLYEFFGEALGGRPADPRAYHPDGRVDYAARRKSPEFA
                     AGIDRLLELVRVANIVLMCAEEDPLQCHRFLMICPALLERGVVPAHLRRDGVLESQRD
                     AEDRLLELHGRTAFATGSLFVSERDSAIEEALGLQAKEFAFRASPEAIEYF"
     gene            5923..6432
                     /locus_tag="VGR03_11685"
     CDS             5923..6432
                     /locus_tag="VGR03_11685"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEV1994983.1"
                     /translation="MRYLLCTIGFAGKTAQEFLTLLKEAGVERLIDIRQNRGGQLSGY
                     AKHPDLAYFLHEIVRIDYIHEPLLAPTPDLLKTYRKTKDWLGYETAFLTLMRARGVPG
                     ILDTSSWSSKVVLLCSEAGPEKCHRRHVADLIAERWRGQGHDVEIRHLVSARAKPATS
                     RRKKRDAAP"
     gene            6429..7148
                     /locus_tag="VGR03_11690"
     CDS             6429..7148
                     /locus_tag="VGR03_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994984.1"
                     /translation="MTKLLITEITRMGPAFCVIGLQREKEKIQSIRPVPSFANGWAHF
                     PHHRGDILECALAPFPVPGAKPHVEDRVATRGFEKSSTVPEAETVMYLRKAETAQSLP
                     DLFGCPIHENKKGSGLHAMPHEAKRSICGCETQNLRLELYANELRATLALPSGEALRD
                     LPVVDRDWRDFIDASLSPGRGANRAARLERFLNSQFHYKIMSCPHHFIRLGLTRPFHD
                     LCWLMLDTLFPLPRPEWLEEF"
     gene            7148..8452
                     /locus_tag="VGR03_11695"
     CDS             7148..8452
                     /locus_tag="VGR03_11695"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEV1994985.1"
                     /translation="MTRPPNTHSVAKDTLRHLLEECFRREAGLSVPIPDENADLIETG
                     ILDSMGWVSFLRTVESASGAGELGSSLSERSASFAAVLAALRNRGPLTADASHDEAEP
                     NAFNTVVPVILTGSSAGVGSRIVPSEEADRAFGMPAGKLRNRAGIESLAYAAEGENEL
                     TLAARAAQEALRAAACGAQELDWIVATSETHHDYPSLSAQLHSRLLVRENCGALDVGG
                     ACLGLLNALVVAQSLIGSGHAQTVAVVTADVHSRTLTPGRVAGEFGGLFGDGASAFIL
                     RSAARSDSTECYRLGEFLFGCAGQYAAAIQVSDTKDGSLTVQFDGEALSRAAITRLEK
                     VLASVELRSGIPRTSVGAFATHQPNPRLVALLAKQCGVSPSTFPPVAQVSGNLGSSTC
                     GAALHEALRSASKQVPGDRRPIFLASLGPGLLFGGGWLTPHD"
     gene            complement(8564..9781)
                     /locus_tag="VGR03_11700"
     CDS             complement(8564..9781)
                     /locus_tag="VGR03_11700"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV1994986.1"
                     /translation="MKIRSKQICASALAMFYGVWFAPVAQAYSFDMIVPDVRLPAAVS
                     GGSACPVRTHQLTSAGSIAVRWSTALNTNPVTIVTQNQTASGRLTEIEQAVAQSLAVW
                     TGVSGTTLVPATLTPLTRTATQNACGPDGVNSICFDQADMAFTPGVLAFTRVITTDRL
                     GVQVGGSAVSTQLGQILDADIYFNPSDSRTAFATPQALAAVPTAYDLESVLTHELGHF
                     LGFGHSAVWSAILFPFAPAPGTFSGMRPTTQQPDAPLGDDDRTGLRVLYPDLADVVHR
                     GSVRGQIFPANPLALPASPPGVTGVFGAQVVAVNATSGAVVGATIGGWSCTAPGPAQF
                     DGTYEIDGLAVGHSYTVYAEALNGAVDPSQFDNAIVALCRNTVSDSGWPPLQGCVVPA
                     VDTSFTARTRPGP"
     gene            complement(9809..10375)
                     /locus_tag="VGR03_11705"
     CDS             complement(9809..10375)
                     /locus_tag="VGR03_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994987.1"
                     /translation="MSFVQRRRFLWILFLAGLALLAIAANATTLSRLRFEELVNQATA
                     VARLRCIGVESRWENGEIWTETRFEIVEVNKGLLPGVVSVRMLGGSIGNLHSRIDGVP
                     AFRPGEEAYVFLWGREGEPFRVLGWSQGTFRISRDVRTGMERVTQDSAAAPVFDPRTR
                     QFRHGGIRNLPVAIFQLKLRKALEEKTP"
     gene            complement(10382..12124)
                     /locus_tag="VGR03_11710"
     CDS             complement(10382..12124)
                     /locus_tag="VGR03_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994988.1"
                     /translation="MSGHYTAPRTLPSPASTTLTAQSVADPSKQISAALAITSNFSLQ
                     LSAPFSVPAGGTATIVATLTPVPGSNPSTTLAWALSGPGCSGSSCGTLTVVTTQTAGG
                     NPVADSATYTAPATAPSPNTVTVTVTPQADPSKKAQATLAIQAGVNVSVSPATATLAA
                     NHRVTLNAQVNGTSNTGVIWSVNGIAGGNTTFGQICAVSSNPCQTVTSTTTPQVEYRA
                     PGAIPSPNPVSATAVSAADSTKSASAQITVINHVLVSVLPGSATLAPLAVQGFTASVL
                     GTSNQNVVWQVQGAACSTAGICGTINANGTYTAPSSAPTPDAIQVVAISSDDTSQSGI
                     ANVTISTGANILTLHPSSVYAGAAQGFTLRVDGSGFAAASPGPASAMLIGGTARVTTC
                     ISTLECTAPVTAADVAVAGSLSIQMQNPNGTKSNAVALIVAAPNVSDEVIALSSAAPS
                     ATGKDIVVVDPTTAGVSVPGNDLDLDVAALGLFVTSSNSCTLGGSAIPLQRPASGTST
                     ADICIFSQSGFDTSMTYTISGSGDVAVISKQPAGSGLSTSRCKSRRTPRQVRARFSYR
                     TQILTRPPRAACWR"
     gene            12224..12601
                     /locus_tag="VGR03_11715"
     CDS             12224..12601
                     /locus_tag="VGR03_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994989.1"
                     /translation="MRRAKLSEMSLVIAKVPEPDFVESAWLVAVTCTVAGDGRSVGAV
                     YTPRDVIVPSVAFPPGTPFTLQLTAVSVVLVTVAAKVAWPPSTTDPLAGFTVTSIVGG
                     AVEVAASSPHRSRTSTLPPQEVR"
     gene            12953..13330
                     /locus_tag="VGR03_11720"
     CDS             12953..13330
                     /locus_tag="VGR03_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994990.1"
                     /translation="MPRPDAVRRVKSYSAADGYVYQYYFFEGNRAQRGSNPGGEFTYV
                     VSADRRSAFPFKIFVLQSALDAWAKLNGRPLTSSEEYAVAKMRLFQAFDEGSVQAPPE
                     GQQPGEVLVDESNLEELLGQLGI"
     gene            13447..14094
                     /locus_tag="VGR03_11725"
     CDS             13447..14094
                     /locus_tag="VGR03_11725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1994991.1"
                     /translation="MQELVLIQPGSTVDRFAVAHDNDRFAVAHDKENESVREAAQADT
                     RHEFEERLAECGPLAYRVARGVLRNTADAEDVAQEAMLRAYRQFDRLRDRNRFRAWLV
                     RIAFRLALDRLRSGKRRELRDMLWSQPEHQPRAANAEDLAASNEFQEHLESALAELPE
                     KLRLVLLLAAMEGHTIDEIASMLGIPVGTVKSRIFYARRKLAEKLRCHANTIRTR"
     gene            14064..14759
                     /locus_tag="VGR03_11730"
     CDS             14064..14759
                     /locus_tag="VGR03_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994992.1"
                     /translation="MPCKHYKNALIEAAASGAQPQGELRAHLADCASCCATFAQEQAL
                     FSSIDAGLYVTANAELPASLLPRVRARLDEEGIPRRNWVTAGFVLASAAAIVIALFTA
                     RVVWRTNGEHQPVESVANKSVSAPGVQPPQGQFAIAPPVKKDSTSQLQVPLVRNPVPP
                     RAFSTRNSMPEVLVPRDQEVLLVHYADQWRGRKRAPLVAADSDETTLTSLQVAPIQIA
                     QLDVKLLAEEKPQ"
     gene            14815..15378
                     /locus_tag="VGR03_11735"
     CDS             14815..15378
                     /locus_tag="VGR03_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994993.1"
                     /translation="MKRLIYLCVLGALCLPLQLVRAQEKSKAEDRIKVPTPIKVQIVF
                     TEFDGEKKISSMPYSFVVITDEKMGGNYSTSLRTGVRIPIEVDGKDQKTTYMDVGSSI
                     DCGIRTEDDGRYHINLSFDRSALYPNKSSEGERLVAEPNGLPLIRQFRAAENLILKDG
                     QTSENLLSTDPLNGHTLRVTVTINVQK"
     gene            complement(15391..16305)
                     /locus_tag="VGR03_11740"
     CDS             complement(15391..16305)
                     /locus_tag="VGR03_11740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEV1994994.1"
                     /translation="MKILVMGSSGLVGTALGSALARGGHTVCRLVRPQSVVGSGTKEG
                     FAVAWNPVTGELGGAGVGADAVVNLAGASIADGRWTTQRKELLRASRIDATRALVGAL
                     AKMNARPSVLVSASAIGYYGDRGDETLTEESKPGADFLAGLAQEWEAEALKAEALGIR
                     VVLARFGIILAREGGALPKMTLPFKFGAGGKLGSGQQWMSWVTLEDVVGILRFAIENA
                     SLRGAINLVSPQPVQNAEFTKLLAKAMHRPALFPAPAFALRLALGEMADALLLSSQRV
                     LPGALEKLGYRFLHANLASALTAVLARS"
     gene            16537..16803
                     /gene="moaD"
                     /locus_tag="VGR03_11745"
     CDS             16537..16803
                     /gene="moaD"
                     /locus_tag="VGR03_11745"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEV1994995.1"
                     /translation="MPPVSQTIRVKVLFFGRLKELAGLAEDSIEFADATTIEQLFALY
                     AARHPDLAKYRSSVVASRNQEFAAWDTPLHSGDEIAFLPPVSGG"
     gene            16813..17289
                     /locus_tag="VGR03_11750"
     CDS             16813..17289
                     /locus_tag="VGR03_11750"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEV1994996.1"
                     /translation="MATLPHDDIYQLVREPIDMAALAHHVRAPEDGAIVTFDGFVRNE
                     SHNRPTLYLDYEAYESMALAKMREIGVQLHEKYRIHRVAVVHRLGRLEIGETSVFIAV
                     SAPHRAAAFDACRFAIDNLKRTVPIWKKEYFEDGAVWADGELPPAPAATPHAKPAS"
     gene            17314..18429
                     /locus_tag="VGR03_11755"
     CDS             17314..18429
                     /locus_tag="VGR03_11755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV1994997.1"
                     /translation="MNLTSMTPFRPHSTLLFAVPALLFAGIAFSQDKPDRPPWAQKPK
                     STSSANSNSKPTTTSSSTAAPASSPDGEPGKTVQPTPPTSTTDSQDAAAQRGRIRVNV
                     NLVNVFVSVLDEKNRPAPDLPREAFQLFEEGVQQKIDKFESETSQPLDLAIMIDSSLS
                     AHKEISFEQEAAAHFIRQVLRPGDRLAVFAFDENVTQLAAFSDNVAELQAAARRIPVG
                     AGTSIYDAVLLGSRALERRGDGRRRVIILVTDAGETTSRADFDAARKEAVLSTTLLYT
                     IVIRPVKNESGRNTAGEHALETMTDTTGGAMFYPDTPQELGAIFDRIDRELRTQYLLA
                     YYPTPRGPANTYRSIQVTVTPADHHVRHRKSYLTGPQ"
     gene            complement(18662..18847)
                     /locus_tag="VGR03_11760"
     CDS             complement(18662..18847)
                     /locus_tag="VGR03_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1994998.1"
                     /translation="MVRVYGKVMEDKDKIPEISVEYVQVWPWLTFTLTDLGVEDCTNP
                     RWLKYCKRCKGGRIYDP"
     gene            complement(18902..21592)
                     /locus_tag="VGR03_11765"
     CDS             complement(18902..21592)
                     /locus_tag="VGR03_11765"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV1994999.1"
                     /translation="MSNTHPNNPPLRFGIFELGVDTGELRKSGRSVRLRPQAAKILVL
                     LASRPGQLVTREELREKIWGSDTFVDFEHGLNLCIREVRAALDDDADTPRYVETLPKR
                     GYRFIAPIQETNQQQTLGRYRLTGKIGAGGMGEVYQGQDLRLGREVAIKVLPPGALQD
                     ENARSRFRREAELLCKLNHPNIATVYDFERQGDSDFLVMEYIRGETLSDRLCAGPLPE
                     KDVLRLGLQLADGIAAAHEHGVIHRDLKPGNLRVTPDGWLKILDFGLARAIKPAIENN
                     ATQTATQTTDVEGTLRYMAPEQLRGQTIDGRSDIYAVGSILYEMTTGQVPFDDKLTTA
                     LVEAILHQPPRPPRQLNSSISPRLEDIILKCMEKDSGNRYQSAKELAVDLRRLAPSTV
                     MTAMAASPRRFSWHTARLFAVIFSVIAITLAVTNPGEWRARLFGGLQPVRTIAVLPLQ
                     NLSGDAAQDYFADGMTEALTTDLARMESVGVISRTSSMQYKGSKKSLPVIARELHADA
                     VVEGSVQRSGSLVRVTAQLVRAPDDRHLWADTYERDFRDVLALQDEVASAIAKQIEIR
                     LGGPPPAPAPKVQPVSPEAYETYLRANSYLDSFDLQKSIDYYHQAIKLDPNYAPAYAH
                     LAQAYSFLAFFGAVSPSEGWGKAKEAAMLALQKDERLPEGHGALALVKLHFDWDFPGA
                     EQEFKRALELNPSDADVRHDHAHYLMAMGRLAESEAESKRAVELDPVGDGLNSCLCWH
                     SFAARDYDAAVRLAEKFLKSQPNDPWELTILGWTYEQKRMPDQAVAEFKKAVEATNGD
                     PFFVAALGHAYAVAGNRREAEKILQTLSDRAKKSYVSPFDVALIYAALGEKDKAFALL
                     DKAAAEHSTFLVYSKWEPRLDPLRSDPRFQDLLRRIGLAS"
     gene            21798..22832
                     /locus_tag="VGR03_11770"
     CDS             21798..22832
                     /locus_tag="VGR03_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995000.1"
                     /translation="MFSASKVSTSHRLFLAALLLFAGATFASAARAQVALSTLDFPGG
                     RAAAIHGTTAAETPSEPQQAAGGQAEVEPDAASALQFIPTDFPGATLTSPQGINPAGD
                     IVGIYDDASGKRHGFLLSRGNFTSIDYPGAVATDARGINRHGHIVGAYINAPGGLQNM
                     HGYLLSHGSFSEVQFPGYLGTIAQRILPNGNIYGCNHNTDFMASMHGYVRTAEGYTAI
                     DVPASMHNGATPDGSTIVGLYADMMGMTHGYLIEEGEFNSFDVPGSNATQGWDINPQG
                     DIVGDFRDTAGHFHGFLRTDDGYTTIDFPAAAQTVARGINRRGDIVGRYTDTNGKLHG
                     FLRSRTKKEE"
     gene            22956..23156
                     /locus_tag="VGR03_11775"
     CDS             22956..23156
                     /locus_tag="VGR03_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995001.1"
                     /translation="MFRGFQYRKGQFAPIDIPSAKSTHPFGINLEGDIVGAYTDQNGK
                     THGFLLTRGEHERRDDEREDDE"
BASE COUNT         4755 a         7531 c         6449 g         4801 t
ORIGIN      
        1 tgctgctgct cgacgtcacg ccgctttcgc tcggcatcga gacctacggc ggcgccgttg
       61 caaaaatcat tccgcgcaat tccacgattc ccgcgagcgc gcaggagctt tacaccaccg
      121 gcgtcgacaa tcagaccggc atcgacatcc acgtcctcca gggcgaacgc gaactagcca
      181 aagattgccg ttcgttggcc cgcttcacgc tgaaagtgcc gcccgcgcct gccggtctgc
      241 cgcgcatcga agtgaaattc ctgatcgacg ctaacggcat cctgcaggtt ggcgccaagg
      301 atcttcgcac cggcgaacag cattcactcg aagttcagcc cagctacggc atcaacgatt
      361 ccgaaatcga gcgcatgctc gaggaatcca tcgaatacgc tgaacaggat ttcgctgaac
      421 gccaggtcat cgaagcgcgc acggaatccg agtcgatcct gacggcgacc gcgaaagcgc
      481 tggccaatcc gcaatctgcg agcttgccgt ccgaggagcg cgccaaaatt gacgcgagtg
      541 tcgctgcatt gcaggattcc gtcgtcggca gcgactacaa attaattcgc aagcgcatcg
      601 acgatctgaa tcagacgacc gagcatcttg cggaattgct catgaactcg gcgatttcga
      661 cagccctgga aggccgtaag ctggccgagg tctagagttt ctttgtaggg gcgcagcacc
      721 gctgcgcccc tacttggcgc gatgttagcg ccgcgagagt cagaggggga aacaaaatgg
      781 gcggcagcaa cccatacatc gaggaagtca aatacaaacc cgcaacgaag aaattcaaag
      841 tgacatttct tcccagcggc aagaccatcg aagtcgaccc ggaaaaaatt ccgtacggcc
      901 acaacggcct cccgggcagc attctggatt tctccgaggg cctgaaggcg ggcctcgacc
      961 acgcttgcgg cggcgtctgc gcctgctcga cgtgccacgt catcgtgaga gaaggcctcg
     1021 agtcctgcaa cgaagccacc gacgccgaac tcgacgagct ggacgaagcg cccggtatca
     1081 cgccgaaatc gcgcctcggc tgccagaccg tccccgacgg caccaccgac atcgtcgtcg
     1141 aaatccccga gtggaacaaa aattacgcca aggaactgga tcactaattc tgttgtagcg
     1201 gcgggtttat cccgccatct gttctctgcg atctctgtgc cctcggtgtt taatcttttt
     1261 cgggagtgga catgccgccg acattcggtt gggacaatct cgaagacatc gccattggct
     1321 tggccgataa attcccggac accgatccgc tcaccgttcg cttcaccgac atgcacaaat
     1381 ggatcaccga gctctccggt ttctccggcg acccggccac ctccaacgaa tccaagctcg
     1441 aagccatcca aatggcctgg cacgaagaat ttcaggaccg ccaatcgtag ctcttcgctc
     1501 caccggcaag cccggcatcg gcaccccagc gatcggtcat cctgagcgaa gcgaccgaag
     1561 gttccgaccc tgtcggaaag gatctcaact tatccattct ctgaggcatg gcctgaggca
     1621 gtcgtgccgc aaatcgccct tgaatttctc tgccgattgt ggtatataca tccttggcaa
     1681 aaggtgtgca ggcccttcgt ccagatctcc tagaatgtgt ccgaaattgc ctcctgcaac
     1741 ttctttagaa tgaacactta taaaagtgtt tattctaaac aacttaaaac cctttaaaat
     1801 gaacacttct tcaaaaacag ggggtagggg ggtgccatgg ttgactggaa tttcggcctt
     1861 cggcccatcc cgtcaagcgc tgccacccgc cgtgcttgtt cgcgccggcc atttcccgta
     1921 tcctaataaa gtcgtaaccg cagcaagtgt ggcgctatgg tgcaacggtt agcacgtagc
     1981 cctttcaagg ctaaaatacg ggttcgattc ccgttagcgc taccaactta cttttgaatt
     2041 gactctgctc tgcgccggat ggcagttgac cagccgtaac ctccggctct catccgccgc
     2101 aggttcccca gcaacaaatg tcctgacgtc ttttctcaag gagccctttt gagctacgcc
     2161 gaaaagcatc tcatcgcggg tgaaaccgtc cagtatgaga cccggctgca ctggattgtg
     2221 atgttggggc atgttttgat cgccgcagtc ctggtgctca ttggtgtgag cttgctcctc
     2281 acgccgtgga gttctgtaaa gggcggagaa gcttctcttg ctggcgcgct gcgatgggcg
     2341 ggcgccgtct gtctagtagt ggcagcgatc tttttcggag ttggtcttgt gcggcgcaac
     2401 gctacggaga tggccgtcac caataaaaga gtcatcgtaa agtcggggct cgcgaatcgc
     2461 cgcaccatcg agctccttct gccaagaatc gaaagtatcg cggtcgaaga gcctgcgctg
     2521 gggaggctcc tgggctacgg aacagtcatc gtgcgcggca cgggtgggac gcccgaagtt
     2581 ttttcgcaga tcgcccggcc cttggaattc agagagcaag tccagcgcca gatcgctgga
     2641 gaaccaaaat cctcctgagc gattgcgtct tccgattctc tggtgctcga cggccgttgg
     2701 agcgatttag aaggtaaact tcatgcgcgc cgagacactt cgaggggtag ccaccgccgc
     2761 cccggaaaac aaacttgcaa aattgatcac gttgagccgg tttgccaggt tcgccacctg
     2821 aatctgcagc gcggcgctct tctgttcctt gtgatagatt tccgcgcccg ccgccagatc
     2881 gacggaaaaa ttcggccgca cgcgcccgcg agccagatcc acgcgatcca gaatctgttg
     2941 cccgaaggct gccagcagat cactgacctt ggcgttgcca acatcagccg gcaacccgct
     3001 gccgtactgt gcgcccatcg ccagccatgc gtgccgcggc gcttgaaatc gcacgcgagc
     3061 ccgcagcgaa ttccgttgat cttgggtcac ggcgaacttg ctcgtatcgg tcagcccatt
     3121 ggccgcgtca ctcccaagaa acaacccgcc ggtgattggc ccttgcccgt agccggattg
     3181 attcgcatag ctcaagtatc cggagaagcg tctccactgc gggacttcca accggatttc
     3241 ttctccgaag atccgcgccc tcgcgaacgc aatcggaaag ctcacgcccg tatcaagcag
     3301 gacgtcgtca tcggaatatt ggcgaaaatc ccggcggaaa acgttggcat ctagccgcag
     3361 ctttcccagg aatgccttgg tcactccgcc ttcataaaaa ttaccccgcc ccggcctcac
     3421 cggcaatcgc aacaccgaag ggctgatcgc atcgagcagc agcgaactcg caagaagcag
     3481 gttctcgata gcgggcgttt gaaaaacccg atcgtacgag gcatgaagca gcaggttgag
     3541 tgccggcacg aatctcgata cgccgattct cggactccat gccgattcgt gcaccacaaa
     3601 cccgtaatga tcgaacctca gccccgcgct gacgttccac cggcccaggc gcatctggtc
     3661 ctgcaagtaa atcgaaggct cgacatccaa tttccgctga tagggaaacg gaaaggtcag
     3721 cagcgtcccg ggaaggaatg cacccggatt ggtgatcgcg tattgcagcg cctcgtgaac
     3781 cgaactgaaa atactgtcca cacccgtttt ccaatcgtga tgcccgtggt gccccgcgag
     3841 gtcggctcgc aggtacactt cgcggtagcc gcgatcctgc gaaacaatca cgggcgtcgc
     3901 gaacctgttc gatgtcagcg tcgcgctcga atcccgcacg cttcccgaaa aactcaaaaa
     3961 caaatcagac gatatcgtgt gctgaaagaa aatctgtccg ctcgtctccg tgttctggat
     4021 atcctgccgt tggcgggctt gctgctgcac cagctcgttc ggcacgagga accggacgac
     4081 gttgtgcatc acgctcacgc gcagccggtc gctcgttgaa aaatctcgtt cgtaggaagc
     4141 cgagaatcct gcggcatttc cccggttcgt gtaattcgcg agcaccggcg gatcgagata
     4201 acggtcagtg tgaaatcctt cgccacctac cgaaaaccgg tttgctcccc gcgcatagga
     4261 aattcccgcc gccccgttct gcgtggagaa acttccgccg cccgcatcca agcgcccgtg
     4321 ccatccggcc tgaacatctt tcttggtcgt gacttcaacg atgccgccca gtttccgtcc
     4381 atattccgcc ggaaaacttg ccgtcaacac gcgcaaggac tccacttcgc ccgcatcaaa
     4441 cgagggcgca aacgccggcg accgattctg cgtcagcggc agcccgtcca ccacgaattg
     4501 cacgtcgtat tccgagccgc gcgggtgcag cacgccgttg gcttcataca cccatcccgg
     4561 ctgttcggag accaggtcta gcagctcgcg tcccggttgc gccgcgtcat gttcctcgat
     4621 cgtttgccga ccgatggagt acagcgttcc cgttgcatag ggatccacca gcgtcgccga
     4681 atcgttcacc tgcacctgcg tcgtcaccgg cgcaacgccc agcgtcacgg ccacgcgcac
     4741 tggcacttcg gaccgaattt caatcagatc gctccacgcc gcgaatcctt ccgcctgcaa
     4801 actcagccgg taaactccaa acggaagatc ctgcgcaacg taacgccctt cgggccccgc
     4861 tacaaaagtc ctccggaacc cattggcctc gctgaccagc tcgccggcgg gcgacaccgc
     4921 ggccccgtgc gggtcatgaa cctcgaggtg cagttcgccc cgcacgcgct gagcccgcgt
     4981 gctcattacg cacaaaaaga aaagcgccac aattccaatg aaccgtgtag tttttctcgt
     5041 gaacatctct atccatcgat ccagaagaga gcagcggctt ctgacccgct ccatttgcca
     5101 cgatacaata cgcatcgcgc cgttatcgga gacatctttt tacgttagca taaagttcat
     5161 tctaccccgc gattccactc cggtccgcgg aggcaatata gcaacacttt gccgaacgcc
     5221 ttgcgcttgc cgcactagcc gtctttcggc agaatacggc tggccctccg agcgcccttc
     5281 catgactccg cggaacgtca caaccggcac gcccttcacc atcggtcatt ccaatcatga
     5341 cctcggcgat tttctcgccg tgctcgccaa atatgatgtc aagacgctct gcgacgttcg
     5401 cagccgccct ggcagtttcc gcttcccgca gttcaaccgc gagccgctcg aagccctcct
     5461 tacctctgcg ggtctcctgt acgagttttt cggcgaagct cttggtggaa ggccagcaga
     5521 tccccgcgct tatcaccccg atggccgcgt cgattacgcc gcgcgccgca agtctccgga
     5581 attcgcagcc ggcatcgatc gcctgctgga attggtgcgc gtcgcgaaca ttgttttgat
     5641 gtgtgccgaa gaagatcccc tgcagtgcca ccgcttcctg atgatctgcc cggcgctgct
     5701 cgagcgcggc gtagtgcccg cgcacctccg tcgtgacggt gtccttgaat cgcaacgcga
     5761 cgccgaggat cgtttgctcg aactgcatgg ccgcaccgct ttcgcgaccg gctctctctt
     5821 tgtttccgag cgcgattccg caattgaaga agctctcggg ctgcaggcca aggaattcgc
     5881 ttttcgtgct tctcccgaag ccatcgagta tttttgactg ctatgcgcta tctcctctgc
     5941 accatcggtt tcgctggaaa aacggcgcaa gagtttctca ccctcctcaa ggaagcgggc
     6001 gttgagcggc tcatcgatat ccgccagaac cgcggcggac agctttccgg ttacgccaag
     6061 catcccgatc tcgcctactt ccttcatgag attgtacgca tcgactacat tcatgaaccg
     6121 cttctcgcgc ccacgcccga tctgctcaaa acctaccgca agaccaagga ctggctaggc
     6181 tacgaaaccg ccttcctcac actgatgaga gcgcgcggcg ttccgggaat actcgacacc
     6241 tcaagctggt cttccaaggt tgtgctcttg tgttccgagg ctggccctga aaaatgccat
     6301 cgccgtcatg ttgctgacct catcgctgag cgctggcgcg gccagggaca cgatgtggaa
     6361 atccggcatc tcgtctcagc ccgggcaaag ccggcgacct cgcggcgaaa aaagcgcgac
     6421 gcagcgccat gaccaagctg ctcatcacgg aaattacgcg catgggcccg gcattctgcg
     6481 tcatcgggct gcagcgcgag aaggaaaaga ttcaatcgat ccggcccgtg ccctcattcg
     6541 ccaatggctg ggcacatttc ccacaccacc gcggcgacat cctcgaatgt gccctcgcac
     6601 cctttcctgt tcctggtgcg aagcctcatg tcgaagaccg cgttgcaacc cgcggcttcg
     6661 agaaatcttc caccgtgccc gaagctgaaa ccgtgatgta tctccgtaaa gccgagacgg
     6721 cgcaatcctt gcccgatctc ttcggctgcc ccatccacga aaataaaaag ggcagtggtc
     6781 ttcacgccat gccgcacgaa gccaaacgct cgatctgcgg ttgcgaaacg cagaatcttc
     6841 ggctggagct ttacgccaac gaacttcgcg ctacgctcgc gctcccttcc ggagaagcct
     6901 tgcgcgatct tcccgtggtc gaccgcgact ggcgcgactt catcgacgcc tctctttctc
     6961 ctggccgcgg cgccaatcgc gccgctcgcc tcgagcgctt cttgaattct cagttccact
     7021 acaagatcat gtcttgtccg catcacttca tccggcttgg ccttacgagg ccgtttcatg
     7081 atctgtgctg gttgatgctg gacactttgt tcccgctgcc caggcctgag tggctcgagg
     7141 agttttgatg acccggcccc ccaacacgca ctccgttgca aaagatactt tacgccacct
     7201 tctcgaggag tgttttcgcc gggaagccgg tttgtccgtc cccatccccg atgaaaacgc
     7261 ggatttgatt gagaccggca tacttgattc catgggatgg gtaagttttc tccgcactgt
     7321 ggaatcggca agcggggctg gcgaacttgg ttccagtctc agcgagcgtt cggccagctt
     7381 cgcggccgtc ttggcggcgt tgcgcaaccg cgggcctctc actgccgatg cgagccacga
     7441 tgaggcagag ccaaacgctt tcaacaccgt agtccctgta atcctcaccg gatcgtccgc
     7501 gggcgtgggt tcgcgcattg ttccttccga ggaagccgat cgcgccttcg gcatgcctgc
     7561 cggaaagctg cgcaaccgcg ctggcatcga atctctcgct tacgccgccg aaggcgaaaa
     7621 cgaactgacg cttgccgcaa gagccgcgca ggaagcgtta cgtgctgccg catgcggagc
     7681 gcaagagctg gattggattg tcgccaccag cgaaacgcat cacgattatc cttcgctttc
     7741 ggctcagctt cattctcgat tgctcgtccg cgaaaattgc ggcgccctgg atgtcggtgg
     7801 cgcctgcctc ggtttgctta acgcactggt ggttgcacaa tctctgattg ggtcgggaca
     7861 tgctcagacc gtcgctgtcg ttaccgccga cgtccacagc cgaacgctca ctcccggccg
     7921 agtcgccggt gaatttggtg gtttgtttgg cgacggcgcc agtgcgttta ttcttcgaag
     7981 cgccgctcga agcgattcca ccgaatgcta tcgcctcggt gaattcctct tcggctgtgc
     8041 gggacagtat gctgccgcta tccaagtcag cgatacgaag gatggcagcc tcacggtgca
     8101 gtttgacggc gaagccctct ctcgtgcagc gatcacccgc ctggaaaaag ttcttgcgtc
     8161 cgttgagttg cgcagcggca tcccgcgaac ctccgtcggt gcctttgcga cccaccaacc
     8221 caaccctcgc ctcgttgcct tgctcgcgaa gcagtgcggc gtttccccca gcacttttcc
     8281 gccggtcgcc caagtttccg gcaatctcgg ctcttccacg tgcggcgcgg ctctgcacga
     8341 agctttgcga agtgcttcga agcaggtgcc cggcgatcga cggccgatct ttcttgcctc
     8401 gctcggtcct ggccttctct tcggtggagg ctggctcact ccccacgatt gagctatttg
     8461 tcctgcatca tgatcggggt agaaaccaaa acccgtgcgc cacacccaca agcctcaaga
     8521 ctatgggcca agaactagaa actgaacact ggcaactttc tcttcaaggc cctggccgtg
     8581 tccgagccgt aaagctcgtg tccactgcgg gcaccacgca tccttgcagc ggcggccagc
     8641 ccgaatcgct gactgtattc cggcacagcg cgacaatcgc gttatcgaac tgcgaaggat
     8701 caaccgcgcc attcagcgct tctgcataaa ccgtgtaact gtgccccacc gccagaccat
     8761 cgatctcata cgtgccgtca aattgcgcgg ggcccggagc cgtgcaactc cacccgccga
     8821 tcgtcgctcc cacaactgcg ccgctcgttg cattcacggc caccacctgt gcgccgaaaa
     8881 cgcccgttac gcccggaggt gaggccggga gcgccagtgg attcgcaggg aaaatctgtc
     8941 cgcggaccga tccccggtgc accacatctg ccagatcggg atagagaacg cgcaatccgg
     9001 tccggtcatc gtcgcccagc ggcgcgtcgg gctgctgcgt tgttggccgc atcccgctga
     9061 atgtgccggg cgccggcgca aagggaaata ggatcgcgct ccaaaccgcc gaatggccga
     9121 agccgagaaa gtgtccgagt tcgtgggtca gcactgattc gagatcataa gctgttggaa
     9181 ctgctgccag cgcttgcggc gtggcaaatg ccgtccgcga gtcgctcgga ttaaaataaa
     9241 tatccgcgtc gagaatttgc cccaactggg ttgaaacggc gctgccgccc acctgaactc
     9301 ccaggcggtc tgttgtgatc actcgtgtga atgccagcac acctggtgta aaagccatgt
     9361 ccgcctgatc gaaacagatg gaattcacgc cgtccgggcc gcaggcgttc tgcgtcgccg
     9421 tccgcgtcag tggcgtgagt gttgccggaa cgagtgtcgt tccgctcacg ccggtccaca
     9481 ccgccagcga ttgtgcaacc gcctgttcga tctctgtcag tcgcccgctc gcggtctggt
     9541 tctgcgtcac gatggttact gggttcgtat tcagcgcggt gctccatcga acggcgatgc
     9601 tccccgccga agtcagttga tgcgtccgca cggggcaagc cgacccgcct gaaaccgccg
     9661 ccggcaaccg tacatccggc acaatcatgt caaacgaata agcttgcgcc acgggtgcaa
     9721 accacactcc gtaaaacatc gcaagggcgc tcgcgcaaat ctgtttggag cgtattttca
     9781 ttgttccggc tctccaatcc taaaatgttc aaggcgtttt ttcttccagc gccttccgca
     9841 atttcaattg aaagatggcc accggcaggt tgcgaatccc gccgtgccgg aactggcgcg
     9901 ttcgcggatc aaacaccgga gccgccgccg aatcttgcgt tactcgttcc attcccgtgc
     9961 gcacatcccg ggaaatgcga aacgtcccct gggaccatcc cagcacgcga aacggttcgc
    10021 cttcacgccc ccagagaaac acgtaagctt cctcgcccgg acgaaacgcc ggcactccgt
    10081 cgatccgcga atgcagattt ccaatgcttc cgccaagcat ccgcacactg accacgcccg
    10141 gaagcagccc cttgttcact tccacaattt caaagcgcgt ttccgtccag atctctccat
    10201 tttcccagcg gctttccact ccgatgcacc gaagccgcgc caccgccgtc gcttggttca
    10261 caagctcttc gaaacgcagc cgtgaaagcg tggttgcatt tgccgcaatt gcaagcaacg
    10321 cgagccccgc caagaacaaa atccacaaga atcgccgtcg ttgcacaaag ctcatttcaa
    10381 ttcacctcca gcacgccgct cgcggcggtc ttgtcaagat ttgtgttctg tatgaaaagc
    10441 gtgcgcgcac ctggcgcggc gttcgccggg atttgcagcg tgaggtggat aatcccgagc
    10501 ccgcgggttg cttggagatc accgcgacat cgccggaccc ggaaatcgtg taggtcatgc
    10561 tggtgtcaaa tccgctctgc gagaaaatac aaatgtccgc cgtcgaagtg ccgctcgcgg
    10621 gccgttgcag gggaatcgcg ctgccgccca gcgtgcacga attgctgctc gtgacgaata
    10681 gacccagcgc cgcgacgtct aagtccaggt cgttgcccgg cacggagact cctgcggtgg
    10741 tcgggtccac tacgacgatg tcttttccgg tggcgctggg ggcggcgctg ctgagcgcaa
    10801 tcacttcgtc ggaaacgttt ggagctgcga cgatcagcgc cacggcattc gattttgtgc
    10861 cgttcggatt ctgcatctgt atggacaagc tccccgccac ggcgacatct gcagccgtta
    10921 caggcgcggt gcactccaat gtggagatac acgtcgttac ccgcgcggtg ccgccgatca
    10981 gcatcgcgga agccggcccc gggcttgctg ccgcaaaacc acttccgtcc acgcgcagcg
    11041 tgaagccctg ggccgcaccg gcgtatacgc tggacggatg caacgtgaga atgttcgcgc
    11101 ccgtggaaat cgtcacgttc gcaattccgg attgcgacgt gtcgtcgctg ctgatggcca
    11161 cgacttgaat cgcatcggga gtgggcgcgg aactgggcgc tgtataagtt ccgttggcgt
    11221 ttatagttcc gcagatgccc gcggtcgaac acgccgcgcc ctgcacctgc caaacaacgt
    11281 tctggttgct ggttcccagc accgacgcgg taaagccttg cacggctagc ggcgcaagcg
    11341 tggcgctccc gggaagcacg gacacaagga cgtgattgat caccgtaatc tgcgcgctcg
    11401 cgctctttgt ggaatcggcc gcgctcactg ccgtggcgct caccggattt ggtgagggaa
    11461 tggcgcccgg cgcacggtat tccacctgcg gcgtggtggt gctcgtcacc gtctggcagg
    11521 gattcgaact gaccgcgcaa atctgcccga acgtcgtgtt tcctcccgca attccattta
    11581 cgctccagat caccccggta ttcgaagttc cattcacctg cgcattcagc gtcacgcgat
    11641 gattcgccgc caatgtcgcc gtcgcgggtg acacactcac gttgactccc gcctgaatcg
    11701 ccaacgtcgc ttgcgctttt ttcgatgggt ccgcttgtgg agttaccgtc accgtaaccg
    11761 tattcggact tggcgccgta gccggcgccg tgtacgtggc cgaatccgcg accggatttc
    11821 cgccagccgt ttgtgtcgtc accacggtca atgttccgca ggaactcccg ctgcagccgg
    11881 ggccgcttag agcccaagcc agcgtggtgc ttgggttcga acctggtacg ggggtcagcg
    11941 tcgcgacgat cgttgccgtc cctcccgccg gcacgctgaa gggcgcggag agttgcaatg
    12001 aaaaattgct ggtgatggcc agcgctgcgg aaatctgttt cgaagggtcg gccacgcttt
    12061 gcgctgtcag cgtcgtgctt gcaggcgagg gaagagtccg cggtgcggtg tagtgcccgc
    12121 tcacgtccac ggttccgcac ccgctggcgc aagccggacc cgcaagactc cagcgtatgg
    12181 ccgtgtccgg acgcccactg cttgttatcg tagcttgaaa ggtttgcgac gcgccaagct
    12241 cagcgaaatg tcgctcgtga ttgccaaggt gcctgagccg gattttgtcg aatcggcatg
    12301 gctcgtggcc gtgacttgca ccgtcgcggg agatggcagg tcagtgggcg cggtgtacac
    12361 gccgagagat gtgatcgttc ccagcgtcgc atttccgccc ggcacgccgt ttacgctcca
    12421 actgacggcg gtgtcagttg tgttggttac cgtcgccgcg aaggtcgcct ggccaccgag
    12481 cacaaccgat ccgctcgctg gctttacggt cacttctatt gttggggggg cggtggaggt
    12541 ggcggcgagc tccccgcacc gcagccgaac gtccacgctc cctccgcaag aagtgcgatg
    12601 accacaatcg tcgttgtctt ggatttcttt ccgttactcc gcgagaggga ccgcatgcca
    12661 tcccaaaagc aggcgaaggg ccagcgaaaa aaagggaagc agggaacgag gattaggcag
    12721 acggttctag gaaactcctc gcaagaaacc gcatggaatc agcagattgc cgcttttcca
    12781 acgccgaata cctatcgccg acgcagcaca agaatgattc tcgctgtttc atctcggcca
    12841 aacttcgttc tgatttggaa ccggacgcgg gcaaagctgt ttcatatcgg gactacctcc
    12901 ccgcgcaaat tcctacgttg acttccctcg ccgcgcctcc aataatccgc ccatgcctcg
    12961 ccccgatgcc gttcgccgcg tcaagagcta ctctgccgcc gacggctacg tctatcagta
    13021 ctacttcttc gaagggaatc gtgcgcaacg gggcagcaat cccggcgggg agtttactta
    13081 cgtggtctcc gccgaccggc gctccgcgtt ccccttcaaa atcttcgtcc tacaatcggc
    13141 cctggacgcc tgggcaaagc tgaacggccg cccgctcacg agtagcgagg aatatgccgt
    13201 cgccaagatg cgcctttttc aggcgttcga tgaaggctcc gtccaagccc cgccggaggg
    13261 ccagcaaccc ggggaagtgc tcgtcgacga atccaacctt gaagagctgc tcgggcaact
    13321 ggggatctag cctggaatgt ctctattgtc cggaacagcc gttcggcact gaaccaatct
    13381 ttaggtggcg gcgtctggtt aggtgtacgg agagggcggg cgctcgcggc tcgcgcagga
    13441 tgacaaatgc aggagctggt tctgatccag ccaggtagca cggtcgaccg ctttgcagtc
    13501 gcacatgaca acgaccgctt tgcggttgcg catgacaagg agaatgagtc ggtgcgcgaa
    13561 gcagcccagg ctgacacgcg gcacgagttc gaggagcgcc tcgccgagtg cggccccctc
    13621 gcctaccgcg tggctcgcgg agtcttgcgc aacaccgccg acgccgaaga tgtcgcacaa
    13681 gaagcgatgc tgcgcgccta tcgccagttc gatcgtcttc gcgaccgcaa ccgcttccgc
    13741 gcctggctcg ttcgaatcgc gtttcgtctc gcgctcgacc gattgcgctc tgggaagcgc
    13801 cgcgaactgc gcgacatgct ttggtcgcaa ccggagcacc agccgcgtgc cgcgaacgcc
    13861 gaagatctcg ctgcttcaaa cgaatttcag gagcatctcg agagcgccct cgcggaattg
    13921 ccggaaaaat tgcgcctggt ccttttgctc gccgccatgg aaggccacac catcgatgaa
    13981 atcgcctcca tgctgggcat ccccgtcggc acagtgaagt cccgaatttt ctacgcacga
    14041 agaaagttag cggagaagtt gcgatgccat gcaaacacta taagaacgcg ttgatcgaag
    14101 ccgcagcgag tggtgcccag ccgcagggcg aattgcgcgc gcacctcgcc gactgcgcct
    14161 cctgctgcgc caccttcgct caggagcaag ccctcttttc ctcgatcgac gctggccttt
    14221 acgtaaccgc gaacgccgag ttgcctgctt ctcttttgcc gcgcgtccgc gcccgcctcg
    14281 acgaagaggg catacccaga cggaattggg tcaccgccgg tttcgttttg gcgagtgctg
    14341 cggcaattgt tatcgctctc ttcacagcgc gcgtcgtctg gcgcacaaac ggcgaacacc
    14401 agcctgtgga atccgtcgcg aacaagagtg tgtcagcacc tggggttcaa cccccgcaag
    14461 gtcaatttgc tattgcgcca ccggtgaaaa aagattcgac ctcgcagctt caagtgcctc
    14521 tcgttaggaa tcctgtgccg ccgcgagcat tttcgactcg gaactcgatg cccgaagttc
    14581 tcgtcccccg agatcaggaa gttttgttag ttcactacgc cgaccagtgg cgcggccgaa
    14641 agcgggcccc gctcgtggcc gcggattccg atgagacaac tctgacgtct ctgcaagttg
    14701 ctccgatcca aattgctcag cttgacgtca agctcttagc ggaagagaag ccgcaatgac
    14761 ttccgctcgg gatgtcaatt ttcgcaacga ttcgaatcgt tcaggaggaa cggaatgaag
    14821 agactcattt atctctgtgt gttgggtgca ctctgcttgc ctctgcagct ggtgcgcgcg
    14881 caagaaaagt ccaaggccga ggatcgcatc aaggtcccga cccccatcaa ggtccagatc
    14941 gttttcaccg agtttgacgg cgaaaagaaa atttccagca tgccgtactc tttcgtggtc
    15001 atcaccgacg aaaagatggg aggcaattac tcgacgagtc ttcgcaccgg tgtccgcatt
    15061 cccatcgaag tggacggcaa ggaccagaaa accacttaca tggacgtggg atcgagcatc
    15121 gactgcggca taagaactga ggatgacggc cgctaccaca tcaatttgag tttcgaccgt
    15181 tccgcactct accccaataa atcctcagag ggtgagaggt tggtcgccga accaaacggc
    15241 ctgccgctca tccgtcaatt cagagcagcc gagaacctta ttctcaagga cgggcaaact
    15301 tcggagaatc tattgtccac cgacccgctc aatggccaca cactgcgcgt caccgtcacg
    15361 attaacgtgc aaaagtaggg cgggattccg ctagcttctc gcaagcacgg cggtcagtgc
    15421 cgaggccaaa ttcgcgtgca agaaccggta gccgagtttt tccaacgctc ctggcaggac
    15481 gcgctggctg gataacagaa gcgcgtcggc catttcgccc agcgccagcc gcagtgcaaa
    15541 cgccggagca gggaagagcg ccggccgatg catcgctttc gcaagcagct ttgtgaattc
    15601 cgcgttctgc accggctggg gcgagacgag attaatggcg cctctcaacg atgcgttctc
    15661 gatcgcaaat cgcaaaatcc cgacgacgtc ctcgagcgtc acccacgaca tccactgttg
    15721 cccagaaccc agttttcccc cggcgccaaa tttgaatggc agcgtcatct tcggcagcgc
    15781 accgccctcg cgcgccagaa taatcccgaa ccgcgccagc actacgcgga tccccagcgc
    15841 ctcggccttc aacgcttccg cttcccactc ctgagccaga cccgccagaa aatcagcccc
    15901 cggcttgctc tcttccgtca gcgtttcgtc gccgcgatcg ccgtaatacc cgatggcgga
    15961 cgccgacacc aggacgctcg gccgcgcatt catcttcgcg agcgctccca ccagcgcgcg
    16021 cgtcgcatcg atccggcttg cgcgcagcag ctctttcctt tgcgttgtcc accgcccgtc
    16081 cgcaatcgac gcccctgcca ggttcacaac cgcatcggcg ccgacgcccg cgccacccaa
    16141 ttctcccgtc accggattcc acgccactgc aaacccttct ttcgtcccgc tgcccaccac
    16201 actctgcggc cgcactagtc ggcacacggt atgcccacca cgcgccagcg cgcttcccag
    16261 cgccgtcccc accagccccg acgaacccat caccagaatc ttcatctttt tcctctgcgc
    16321 ttattaagga ctggcgcgga caaaagacca gcctcttgca cgctacgcaa cccacactcc
    16381 acggtcaagc cattgaagct ttcaaatcca atctttcaga tgaaaatgtc gaatcctccg
    16441 tcgtcccaaa cagatcgggt gctgcaacgt gccgggattg cacaggttga aactgtgtca
    16501 ctggccccct cacccaacgc gggttacaat tcccctgtgc caccggtctc acaaacaatt
    16561 cgcgtgaagg ttctgttttt cggccgtctc aaggaactcg ccggccttgc cgaagattcc
    16621 atcgagttcg cagacgccac gacgatcgag caacttttcg ccctctatgc cgcgcgccat
    16681 cccgacctcg caaaataccg ttcctcggtt gtcgcttcgc gtaaccagga attcgccgcc
    16741 tgggatacgc ctctccattc cggcgacgaa atcgcctttc tcccgcccgt gagcggtggc
    16801 tgacgcatcc acatggcgac actcccccac gatgacatct accaattggt gcgcgagccc
    16861 atcgacatgg ccgcgctggc ccatcacgtg cgcgcgcccg aagatggcgc catcgtcact
    16921 ttcgacggtt tcgtccgcaa tgagtctcac aatcgtccga ctctttacct ggattacgaa
    16981 gcctacgaat ccatggccct cgccaaaatg cgcgaaatcg gcgttcagct ccacgaaaaa
    17041 tatcgcatcc atcgcgtcgc cgtggtccac cgcctcggac gcctcgagat cggtgaaacc
    17101 agcgtattca ttgccgtcag cgcaccgcac cgcgccgccg cctttgacgc ttgccgcttc
    17161 gccatcgaca acctcaagcg aaccgtgccc atttggaaaa aggagtactt cgaagacggc
    17221 gcggtctggg ccgacggcga actccctccc gcgcccgcgg cgacacccca tgcgaaacct
    17281 gcatcctaat tgtcgaccgc ttggcggtcg cgcatgaatt tgacgtcgat gacacccttt
    17341 cgcccccact ccactttgct cttcgccgtg ccagcgctgc tcttcgccgg gatcgcgttc
    17401 agccaggaca agcctgaccg tcctccctgg gcgcagaagc cgaagtccac ctctagcgcc
    17461 aactcgaatt ccaagccgac tacgacttcc tcctcgaccg cggcccctgc ttcctctcca
    17521 gacggcgagc ccggaaaaac cgtccagccc acgccgccga cctctacgac cgattcacag
    17581 gatgccgccg cccagcgcgg gcgcatccgc gtcaacgtca atctcgtcaa cgttttcgtc
    17641 agcgtcctcg acgaaaagaa tcggccggcg ccggaccttc ccagggaagc ctttcaattg
    17701 tttgaagaag gcgttcagca gaagatcgac aagttcgagt ccgagacctc tcagccgctg
    17761 gacctggcga tcatgatcga ctccagcctc agcgcccaca aggaaatcag cttcgagcag
    17821 gaagccgccg cccatttcat ccgccaggtg ctgcggcccg gcgatcgcct ggccgttttc
    17881 gcgttcgatg aaaacgtgac gcagctcgca gcgttttcgg ataatgtcgc cgaactgcaa
    17941 gcggcggctc gccggattcc cgtcggcgcc ggcacttcga tttacgacgc cgtgcttctc
    18001 ggctcgcgtg cgctggaacg ccgtggagac ggccgccgcc gcgtcatcat cctggttacc
    18061 gacgcgggcg aaaccaccag ccgcgccgac ttcgacgcgg ctcgcaagga agccgtcctc
    18121 tccaccacgc tcctttacac catcgtcatc cgcccggtga aaaacgaaag cggccgcaac
    18181 accgccggtg aacacgccct cgaaaccatg accgacacca ccggcggcgc catgttctat
    18241 cccgacacgc cccaggagct cggcgccatc ttcgatcgca tcgatcgcga gctccgcacc
    18301 caatatctcc tggcctacta tcccaccccg cgcggccccg ccaacaccta ccgctccatc
    18361 caggtcaccg tcacccccgc cgaccaccac gtccgccacc gcaagtccta cctcaccggc
    18421 ccgcaataat ccttcgctct tgtagcggcg actttacctc gccatctttc ttttctttgt
    18481 cccagtagca gccgtcttcc gagccgggcg tctttatgtt tggtctcctg ttgtagcgcc
    18541 gtaggcgcac cagaagttag cccagcccgt cagggctggg aagagactat tttgtctctt
    18601 ccagatcaac tccgaaatct ttcgggtccc ctagaacttc gcggtagtac ttctcggtgg
    18661 gctacggatc gtagattcgg ccgcccttgc agcgcttgca gtatttgagc cagcgtggat
    18721 tggtgcaatc ttccactccg aggtccgtga gagtgaaggt aagccatggc cagacctgaa
    18781 catactccac tgagatctcg ggaatcttgt ctttgtcctc catcaccttg ccatagaccc
    18841 gcaccaaggc gagtgacgac ctgcgtgaac tacacgagaa catctctccc acgtagggca
    18901 actaacttgc cagtccgatg cgcctaagta agtcctgaaa gcgaggatct gagcgcagtg
    18961 ggtcaagccg tggctcccat ttgctgtaaa cgagaaatgt ggagtgctca gccgcggcct
    19021 tatccaacag ggcaaatgcc ttgtccttct cgcccagagc cgcgtagatc agcgcgacat
    19081 caaacggcga gacataggac ttcttcgcgc gatctgagag tgtctgaagg atcttttccg
    19141 cttcgcgccg attgcccgcg acggcgtatg catgccccag cgcggcgaca aagaacggat
    19201 cgccgttggt cgcctcgaca gccttcttga attccgcgac ggcttgatcc ggcattcgct
    19261 tttgctcata ggtccatcca agaattgtca gctcccacgg atcgttaggt tgcgatttca
    19321 ggaacttctc cgccaggcgg actgccgcat cgtaatcgcg ggcggcaaag ctatgccagc
    19381 agaggcacga gttcagtccg tctccgaccg gatccagttc cacggcgcgc ttgctctcgg
    19441 cctccgattc cgctagccgg cccatggcca tgaggtaatg agcgtggtcg tggcggacat
    19501 cggcgtcgct ggggttgagc tccagcgcac gcttgaactc ctgctccgct cccggaaaat
    19561 cccagtcgaa gtgtagcttc accaacgcca aggctccgtg accttcgggc agacgctcat
    19621 ctttttgaag tgccagcatt gccgcttctt tcgcttttcc ccagccctcg ctaggcgaga
    19681 ccgcgccgaa gaaggcgaga aaggagtaag cctgtgccaa gtgggcgtag gcaggggcgt
    19741 aatttggatc gagcttgata gcctgatggt agtagtcaat gctcttctgc agatcgaagc
    19801 tatcgagata agagttagcc ctcaggtagg tctcgtacgc ctcggggctt actggctgca
    19861 ccttcggcgc aggtgccggc ggtggaccgc ctaacctgat ttcaatctgc ttggcgatag
    19921 ccgaggcaac ctcgtcctgc aacgcgagga catcgcggaa atcgcgttcg taggtatcgg
    19981 cccagagatg cctgtcatca ggagcgcgaa ccagttgggc ggtgacgcgc acaagactgc
    20041 cggagcgttg cacggagcct tccaccacag cgtccgcatg aagctcgcgc gcgatgaccg
    20101 gaagagactt tttggatccc ttgtactgca tgctcgaagt gcgtgagata actcccacgc
    20161 tttccattct tgcgagatcg gtcgtcaacg cctctgtcat gccatctgca aagtaatcct
    20221 gcgcggcgtc gccggagaga ttctgcaaag gcaacaccgc gattgttcgc actggctgga
    20281 gaccgccaaa taaccgcgcc cgccattcac ctggattcgt aaccgcaagc gtaattgcga
    20341 ttacggaaaa aattacagcg aacagcctcg ctgtatgcca agagaaccgg cgcggagaag
    20401 cggccattgc ggtcattact gtgctgggcg caagcctccg caggtccacc gcaagctcct
    20461 ttgccgattg atagcgattc ccagagtcct tttccatgca tttgaggatg atgtcttcca
    20521 gccgcgggga aatcgacgag ttcagctgcc gtggaggtct cggtggctgg tgcagaatgg
    20581 cctcgaccag agctgtcgtg agcttgtcgt cgaatggcac ctgtcccgtt gtcatttcgt
    20641 acagtatcga gccaacagcg taaatatcgc tgcggccgtc gatcgtctgt ccgcgcaatt
    20701 gctcgggtgc catgtagcgc aatgtgcctt ccacatctgt cgtttgcgtt gctgtttgcg
    20761 tcgcattgtt ctcgattgcc ggcttgatgg ctcgggctag tccgaagtcg agaattttca
    20821 accatccgtc aggggtgact cgcaagttgc ccggtttcag gtcgcggtga atcacgccgt
    20881 gctcgtgcgc ggcggcgatt ccgtccgcca attgcaggcc gagccgcaac acgtcttttt
    20941 ctggcaaagg cccggcgcac agcctgtcgc tcagggtctc cccgcggatg tactccatga
    21001 cgaggaagtc gctgtcgccc tgcctctcga agtcgtaaac cgtggcgata ttgggatgat
    21061 taagcttgca caggagttca gcctctcggc ggaaccggct ccgagcattc tcgtcctgaa
    21121 gtgcgcccgg tggaagaacc ttgatagcta cttcgcggcc caggcgtagg tcttggcctt
    21181 gatacacctc acccatgccg ccggcaccga tcttccctgt gaggcgatag cggccaaggg
    21241 tttgctgctg gtttgtttcc tggatcggtg cgatgaagcg gtagccccgc ttgggtagtg
    21301 tttcaacgta tcgcggtgtg tccgcatcat cgtccagggc cgctcgcact tcccggatgc
    21361 acaggttgag gccgtgctca aaatcaacga aggtatcgct tccccagatc ttctcccgta
    21421 gctcttcccg tgtgacgagt tggccgggac gagaagcaag caacaccaag attttggcgg
    21481 cctgcgggcg tagccgaacc gagcgccccg acttgcgcag ttctccagtg tcaactccta
    21541 gttcgaagat tccgaaacgg agaggcgggt tattgggatg agtgttggac acgcaaacac
    21601 caaagctgtc ggcgcattat actcccaaaa aagcaccaat actcaagtct gcaagcacct
    21661 atgttttcac tgacttcgac ccttacccaa aactaatcct gcgcgaattg accgccatcc
    21721 gattcggccg cagtgtgggg tcacgccgag cccaaaaccc ggcgttgaga tcaagcccat
    21781 ttcacaggag gaatacgatg ttttcagcaa gcaaagtttc aacgagccac agactgtttc
    21841 tcgccgcact actgttattc gctggtgcga cgtttgcgag cgccgcccgc gcccaggttg
    21901 ccctctcgac gcttgatttc ccaggcggaa gagcagctgc gattcacggg actactgccg
    21961 cggaaacgcc gagtgagccc cagcaagcag cgggaggcca ggcggaagtg gaacccgacg
    22021 cagcgtcggc tctacagttt attcctaccg attttcccgg ggctacttta acatccccgc
    22081 aaggcatcaa tcctgcgggc gatatcgtcg gcatatatga tgatgccagc ggtaagcggc
    22141 acgggttctt gctaagccgc gggaacttca cttcgatcga ttaccccggc gctgtggcaa
    22201 cggacgccag ggggatcaac cggcatgggc acattgtggg ggcctatata aacgcacccg
    22261 gtggcctgca aaatatgcat ggctatctgt tgagccacgg tagcttctcg gaggtgcaat
    22321 tccccggcta cttgggcacc atcgcccagc ggatcctgcc gaatggcaac atatacggct
    22381 gcaatcacaa cacagatttt atggccagca tgcacggtta tgtgcgtacc gccgagggct
    22441 ataccgccat tgatgttcct gcttccatgc acaacggcgc gacgcccgat gggagcacaa
    22501 ttgtcggctt gtacgcggat atgatgggca tgacgcacgg ctacttaatc gaagaaggcg
    22561 agttcaattc ttttgacgtt cctggcagca atgcaacaca aggctgggat atcaatcctc
    22621 aaggtgacat tgtgggagac tttagggata ccgccggcca tttccatggc tttctgcgaa
    22681 ccgatgatgg ctacaccacc atcgattttc ccgcggcggc tcaaacagtg gctcgcggga
    22741 tcaaccgccg aggcgacata gttggacggt ataccgacac taacggcaag ctgcatgggt
    22801 ttctgcgaag caggaccaag aaggaagaat agcagcaagt ggcgcgcaga gtaggccaaa
    22861 gcgacggaag catgagagta gagcgggtct cagaacgccg gaagcggcct tgtgcctccg
    22921 gcgttcatcg gtgcgcagcg gatccgacga ctcacgtgtt ccgcggcttt cagtatcgca
    22981 agggccagtt cgctccgatc gacatcccca gcgccaagag cacccacccc ttcggaatca
    23041 accttgaagg cgacattgtt ggagcctaca cggaccagaa cggcaaaacg catggtttct
    23101 tgctgactag gggagaacac gaaagaagag acgacgagag agaagacgac gagtaagcta
    23161 aagggcactg acccagagct ttgcctttcc cattcgagcg gacgacaaac tctggacacc
    23221 tctgggcttg gtccgtgttg tacgtcagct cggtaccgcc cggcttcggc cgggcggcat
    23281 tttaatcacg ccgaagaacg gtgcaaccag cgctatggta atcctggagt cgcggtgccc
    23341 cagaaacaag aggcccacga catcgggaac ggggcgaggt accccatctc gatctgggct
    23401 gattagataa gagagaaggc tccgaacgcg gaccgtgtta gcttttggtt gaggaccgaa
    23461 gctatcggaa cggaaggagc cttatggttc ttattggatg tgattttcac cccagttggc
    23521 aacaagtttc gtggtt
//
LOCUS       DASYVO010000347        18628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_123547, whole genome shotgun sequence.
ACCESSION   DASYVO010000347 DASYVO010000000
VERSION     DASYVO010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 18628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18628
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123547"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VGR03_11780"
     CDS             complement(<1..620)
                     /locus_tag="VGR03_11780"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEV1995002.1"
                     /translation="MAKDCRQRSRQMEITIDTFAKLKQLLDQRIVILDGALGTMIQAL
                     QLEEADFRGKEFAHHPRDVRQNNDLLNITQPRIIEDIHRQYLEAGADIIETNTFNSTA
                     ISMAEYQLQDHVYDLNAAGAKAARRAVETFMAEHPGRSCFVAGAMGPTSRTASMSQDV
                     NSPAARGVTFDELRAAYHEQARGLVEGGVDVLLLETIFDTLNAKAAL"
     gene            870..946
                     /locus_tag="VGR03_11785"
     tRNA            870..946
                     /locus_tag="VGR03_11785"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:904..906,aa:Arg,seq:cct)
     gene            complement(1019..4495)
                     /locus_tag="VGR03_11790"
     CDS             complement(1019..4495)
                     /locus_tag="VGR03_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV1995003.1"
                     /translation="MTLKSILKSFGAGLLALALICGVSAPLPVHAQVVGGTISGTITD
                     SSGGAIPNAKVTIKNIATGIARSVTTNSDGYYTASNLLPGSYEVTISAQGFNTEVKRG
                     IELTVGGLQTLNLTMQVGTITKTVEVTTEAPTVQLSTSDISATVNATTVRELPLNGRS
                     WTDLATLQAGVDAIQTQPSFAVGADRGNRGFGQQLTISGARPQQNNYRLDGASLNDYA
                     NGGPGSVLGGNLGVDAIQEFSVLTSNYSAEYGKTSGGVVNAVTRSGANSFHGSAYEFL
                     RNSALDARNFFDAGTIPAFKRNQFGGSVGGPIVKDHTFFFADYEGVRQGKGISTLLTV
                     PSLNARNGILANGNPPVASCAAIPNTTLLVPGQSNVCIHNSALLYFPLYPKPNGPVTS
                     NPDNAQFTFAGQQVVNENFLTARIDHKFTDKDSLFGTYVFDRTPYSAPDSFDNVMLGS
                     LTSRQIAVVEETHTFNPGLVNSIRFGFNHAAVNNNQGVSAINPAAKDPTLASFAGRTA
                     AQVQISSGLSPLPGGVGSLPTYYYNWNSFQLYDDAFLSRGTHTIKFGGVVERMLLEIR
                     AFSDPNGIWQFKNLTNFLTNNPRRIQGGVVSTVTPRHLRTNLFGAYVQDDWRVRPNFT
                     LNLGLRYEMSTVPTEANGKLANLRKLTDATAHLGDPFFSNPTLKNFEPRVGFAWDPLH
                     NGKLAVRGGYGMFDVLPLPYQYILLTTLAAPFFQYTIVKDPPAPGCTTCFTSTTPFFS
                     GLPSTLPSSRLRTTFVEPNPKRNYVMQWNLNVQYQLTPNLSAMVAYVGSRGLHQPFRV
                     DDSNQILPTLTPQGYLWPQVDAAGNLTSGPNVGLAPEPFNPTFNSVRGMFYQGRSYYD
                     AMELQVSKRMSHGFQMQGVYTWGKSIDTSSATVAGDAFGNSISSLNYFDPKVGRGVAD
                     YNIGRTLVLSGTWQIPTAKSFTGPARWITDGWQLGLIFTASDGVPFTATWGTGSDPAN
                     SLNNDDWAYIKLTGGPGCKSLTNPGNPNNYIKTQCFSVPTAPNLAFWNANCDPVPLSL
                     GLLPGQTFDPLNPANPAPGFTPQPQLPALPCFNLRGNAGRNILVGPGITEVDFSVFKN
                     NYIRKISENFNIQFRAEFFNLFNHANFAPPTTPDNTDIFDGTGTSTGVAGLLSRTTTT
                     AREIQFAIKIIF"
     gene            complement(4808..6712)
                     /locus_tag="VGR03_11795"
     CDS             complement(4808..6712)
                     /locus_tag="VGR03_11795"
                     /inference="COORDINATES: protein motif:HMM:NF014312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HEV1995004.1"
                     /translation="MLKKKKATLLLFLVAAVNNLSSGTKGPAAAAVTAPRALLASSEQ
                     SAGGAAQAQPSLDYQAYRSRIEPIFLKKRQAGVSCYDCHSVLPTRLRLERLSAGSSSW
                     TEEQSRRNFAAVGQLVTPSNPLKSPLLLHALAQEAGGDPMHTGGKFWASQSDPEWQML
                     ADWVRKSSPAPEAVEPAAPSTATHALDLEIFKTRVEPIFLKERPGHARCYVCHTEANR
                     LLRLATLSAGRADWTDEQSQRNFQNALQQVVPGDPTSSRLLMHALAPEAGGDPFHSGG
                     RQFQSQNDPDWLVLAEWVRSSTTRAVAESSPVPIARIYVTNSAGDTVDVVDPATNKVV
                     QVIRGIELPHGVNFSPDGTRLYISNESESVLDVVDRKSGEIQRKIALSGRPNNLAITK
                     DGGRVLVGIRFGPAVLDVIDTTSLQRVKSIPVNGGVHNVYVTPDGKYAVSGSIESMMA
                     TVVDLQTDQAVWEVKFEAGVRPMAFETNPDGSTSRIFVQLSNYHGFAVVDFAKRAEVA
                     RIKLPEQPGGFGIVEGRAGTPSHGIGVAPDGKSLWVNSTLANAVFVYSLPDLKLIGHV
                     ALSEVHPLGLSPTGAVPEWITFTPDSKFAYVSDSAARSVSAIDTKTLKEVALIPVGEV
                     PKRVNTLVLH"
     gene            complement(6765..7835)
                     /locus_tag="VGR03_11800"
     CDS             complement(6765..7835)
                     /locus_tag="VGR03_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995005.1"
                     /translation="MKRSELRRLAMFAAILLAGSFALAGTPNSGYHLLTKYPLGAAPG
                     GTEYWDYITFDASTRRLYISHGTEVKVVDADSGAVLGSIVDLKRVHGIVLVKEVGKGF
                     ISDGGADQAVIFDLKTLKVVGQVKTGGNPDCIIYDPASKHVFTMNGRSGDASVIDPVS
                     GTLVGTVPMGGRPEYAVADGKGMIYDNIEDKNEVVALDSRALTIKARWPIAPAAEATA
                     MDMDVQHRRLFIGGRNKILAIMDADNGKVLQTFPIGEGVDTNIFEPKTGMLFTAVREG
                     ELHVFHEDTPDKFSVIETVQTEFGARNMALDPKTHQLFIDTADFGPAPAPTAEKPHPA
                     RPPIPGTFRLLVYGREKRGASR"
     gene            complement(7905..9668)
                     /locus_tag="VGR03_11805"
     CDS             complement(7905..9668)
                     /locus_tag="VGR03_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995006.1"
                     /translation="MRYWNTKPLLLAPLALLGIALLGMSPQARGRQSTAKPPAYPLPR
                     WPSYLKKLTTIDDMMPHARDLVRNKAGFGGNSPGLGLGLLQPGEEIMIVPDTTAEDLV
                     VQAIVRALAERGVHADIVPNYSLVGVSKEDAEKIRLKTEIRSAEEGFMEAKYYWIERV
                     FTHPEVPKEWLRQKNPGLYDALYPTRDEMTPELQIQWKKLTNESVGAAVRTYLQQHPK
                     IKGVYWGHPGGAFYARTMAPLEGKYLGRCSFTNSWEVVSQVSSFPAEVWQFIEKKTIA
                     MVTPDVDKVVVTDPEGTNVTWDVPEEMAQRWRRGIYWRGHVLMYPDSATGQYGQDFDN
                     YPKTFPDWIPRSPTARASGVIAGTNGSGGFWPHMEIHLQDGHITDVKGGGRYGDVIRE
                     FQHYPHINDATYPNYDTPGYWRLWEVAVGTNPKYFRNPTDFYGGGTAGVYCLTYERYR
                     SGAFHWGFGNELGHDPGSLGTPKKWQKFAEELDLPNGHDFHIQNYFITYKVHRRTSGK
                     WVTIVDRGHLTALDDPEVRALAAKYGDPGRLLAEDWIPDIPGINVPGNYEEYSRDPWK
                     YANAQMQKILAGDSQFDYPRK"
     gene            complement(9897..11327)
                     /locus_tag="VGR03_11810"
     CDS             complement(9897..11327)
                     /locus_tag="VGR03_11810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1995007.1"
                     /translation="MRKAESLRFRMMVLFCTVVGVLLAVSYLAFWALLARVVHTHLNR
                     QLEAAARPVIAHLIAKPDQDNVNQLDLPGEFFELLDPAGRVLQRSKNLPAPFDLKGIN
                     PAASEPVFAIAAIENGEPVRVTLIPFEQGGQRRILAVAIPTFGTNRVLDSFGGIALFL
                     FPLSLLLTAGISAFYVGRSLAPITALTEQAALMARKVANGEGFGRPMAVTSQHDELGR
                     LAETFNQLLQSVDSALRQLRQFVTDASHELRTPLAVLHGETELLLSRPRSAEEYRKTL
                     CVIDDELKKFTRIVEGLFTLSMADAGQLRLESEPLYINEVLEEACALMASRARAKNIV
                     IVRELNQEMACFGDEAFLHQLFLIFLDNAIKYSLADTRVRVTLQRRDSVIRVRFEDQG
                     IGISSEHLAPIFERFYRAAPSGNSEAHSGGLGLAIAQAIARAQGGSIECESTLGAGSA
                     FTVILPDSRPIESIRESELKQKLILS"
     gene            complement(11324..11998)
                     /locus_tag="VGR03_11815"
     CDS             complement(11324..11998)
                     /locus_tag="VGR03_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1995008.1"
                     /translation="MRILLVEDERRVASFIARALRENSYAVDVAETGEKALEMATAVT
                     FDAILLDVRLPGLSGIEVCRELRQQGIEAPILMLTARTLVEQRVEGLDAGADDYLTKP
                     FALAELLARVRALVRRGFHKGGAKLCYADLELDRHRRRATRGKEAILLTSKEFALLEL
                     FLLRAPEPITRSEIIAHIWNYHFDSETNIVEVYINRLRQKIDQNRPAKLIHTVRGVGY
                     QLVMPE"
     gene            complement(12128..13438)
                     /locus_tag="VGR03_11820"
     CDS             complement(12128..13438)
                     /locus_tag="VGR03_11820"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV1995009.1"
                     /translation="MKSWNSKSAMLFLLMGLATALHAQAPAVVAIRAGRLFDSKSGQT
                     LTNQVVLVEGEKITAVGPSGQVQIPAGAQVIDLGQATVLPGLIDGHTHVFGFGLDGIK
                     PGGPPFASNINDTREYRTLLALANAQKDLRAGFTTLRDLMSHGGGYADVDIKKAINRG
                     FFQGPRMQVSTMGLVATGEGILGSPEVSLPRNYQTVDSPWAARQAVREQIRFGADWIK
                     FHSTAEYEFEPDGKLWSDPTFTLEEVKAIVDEAHRHGKKAACHAFGGEGLRNCVEAGS
                     DTIEHALQLDEATADMMKQKGIYLELTAYHYSLADYTVRDAKATGGKYSLEALREKSG
                     RLAIARGVKISFGTGVGPFPHGSQAVEFEYLVKYGMTPAQAIQSATTVAAEMMGWQDR
                     IGSIEKGKYADIIAVSGDPLKDVTELERVKFVMKGGQVVRSDLK"
     gene            complement(13588..14601)
                     /locus_tag="VGR03_11825"
     CDS             complement(13588..14601)
                     /locus_tag="VGR03_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEV1995010.1"
                     /translation="MKKQYLLLMTALVLFAVPAQCQQTAPLKQVQTFTFPATVKGHFD
                     HFEVDLKGNRLFTTPEDYKAVVVFDLKTGKLVHTITGIERPHAVLYREDLNRIYVTDG
                     QAGDVKIFDGKTYGLIKSVKLLADADSIGYDSATKFLYIDNGGGDVHQTYSMLSVVDT
                     TAGTKLADIKVDGETIEAMTLEKSSARIYVNIRTKNSIAVVDRAKREVVATWPVTKCK
                     VNVAMALDEANHRLFAACRDGQVAVLDTESGKEVAALAITTGVDDVVFDPASKRFYAS
                     ADGAVDVYGQTDANNYKHLGKVTTGPVGRTARLVPELGKYFVAVPQHGTTNAEVLVFD
                     VQR"
     gene            15014..16330
                     /locus_tag="VGR03_11830"
     CDS             15014..16330
                     /locus_tag="VGR03_11830"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1995011.1"
                     /translation="MPDRSPTRVRWHILAILTLIMVATALGRLNLGIAGKYMQEEFQF
                     TTQTMGWILGAFAFGYALFQVPCGWAGDRFGPRITLTLAILWWATFTMFMSLVPYLRG
                     SSWLNLAWAFGLVRFLTGAGEAASYPNANKIVAFWTAKAERGIGSSLLLGGVGAGGVL
                     APILFAGIMQRWGWRSSFVVSGVLAAVVAAIWFFFSTNRPEEHPRVNSEELEILGSTL
                     RGHGIHSFRLKGTPWRQIFSSRSVWALILSYFFHGYTPYIYFAWFFIYLTRVRGLTVT
                     QGGLWGSTPFLAMTLMAPLGGWLSDKAVVRFGRRRGRQSTVWVGMTCSALFLWAGSHA
                     LNNTSAILLLATAAGFSNFAAPSWWATCIDLAPNHSGSLSGLMNTCANIAGGISPILT
                     ARIATQFGWTKALDFAALVSFTAGIIWVFVNASDNLEAGASVEAPS"
     gene            16413..17282
                     /locus_tag="VGR03_11835"
     CDS             16413..17282
                     /locus_tag="VGR03_11835"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEV1995012.1"
                     /translation="MTTAFREMLKAPGIIHAPIAYDPLTARIAERVGFRCLDLGGYAL
                     GASSCVAEPLLSLEEVLRATRRITAAVAIPLMVDGGAGYGEPAHVIRTVQELERAGAA
                     AVHLEDQVYPKRIHYHKGVEHVISAGEMCQKIRCAVRARRDPDFVIAARTDAMRTDGF
                     AEGVRRANLYAEAGADMVMIFPNTSEEARKGPAEVRAPLIYVNSEGNRLGRPIFAAGE
                     LEAMGYKMLNDAISAIAVMFKSVSELFLRLKETGRTGMDQAVFVKIRKEIEDTIGLEE
                     YYRIEEETVENRE"
     gene            17579..>18628
                     /locus_tag="VGR03_11840"
     CDS             17579..>18628
                     /locus_tag="VGR03_11840"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV1995013.1"
                     /translation="MQRVILLSIDGMHSLDLANLIKERPESTLAKLSQHGVTYSNAST
                     SFPSNSWPGLLSMVTGGSPNVTGVIFENSYDRSLSPPGSDCTKIGTAIIFDSSIDKDR
                     DALDGGGGIDPNKLPRDPKKGCAPVFPHSFIRVNTIFEVIKKSGRRTAWSDKHPAYEF
                     VNGPSGTGVDDLFAPEIRASHATRSVEKAKAYDDSKVAAILSEIDGKDHTGASQVGVP
                     AIFGMNFQAISVAQKTTGNGYIDGSGSPSPALHDAFTHTDQSIGKMMARLRERGLFDS
                     TLVIITAKHADSPIDPAKLKLADLGLIPKTVAAIGKGLLAGVEQDGSVAMLWLSDQER
                     TADVVNALRKIQAEAG"
BASE COUNT         3691 a         5467 c         5563 g         3907 t
ORIGIN      
        1 agcgcggcct tggcgttcag cgtgtcgaaa atggtttcga gcagcagcac gtcgacgccg
       61 ccctcgacca gtccgcgcgc ctgctcgtga tacgcggcgc ggagctcatc aaaagtcacg
      121 ccgcgcgccg cggggctgtt cacgtcctgc gacatcgagg cggtccggct ggtgggtccc
      181 atcgcgccgg ccacaaagca gctccggccg gggtgctccg ccataaatgt ttcgaccgcc
      241 cgccgtgcgg ccttggctcc agccgcgttg agatcgtaga cgtggtcctg aagctggtat
      301 tcggccatgg agatggcggt ggagttgaag gtgttggtct cgatgatgtc cgcgccggct
      361 tcgaggtact ggcggtgaat gtcctcgata attcgtggct gggtgatgtt gagcagatcg
      421 ttgttctgcc ggacgtcgcg cgggtgatgg gcaaactcct tcccgcggaa atcggcctct
      481 tcaagctgca acgcttgaat catggtgccc agcgcgccgt cgaggatcac gatgcgctgg
      541 tccagaagct gcttcagttt tgcaaaagtg tcgatggtga tttccatttg ccgcgagcgc
      601 tggcggcagt ctttggccac aacaaccggc cttcgctacg cctgcaagat tcggtcacca
      661 ctctgccgtt ctgcggcaca tctgcccgcc gccggacgaa ggggataaat aatcagtgtg
      721 ggagaagaga aaacggaatg gaagtggatt ctccaggcgg gttgagcgtt gaacaaggcg
      781 agccggtcac ggcaagcagg ggccgaaagc ggcggcgcct ccaaatcggg tgcgcgccgc
      841 gtccaaccag tctaaaatag accgagcatg ggcctgtagc tcagatggat agagcagcgg
      901 tttcctaaac cgcgtgtcgg gagttcgact ctccccaggc ccaccatctc agggcaccgt
      961 ggatgaattt cccttcgtca gttatcaact caaccgcacc gacagggacc gccagcgatc
     1021 aaaaaataat tttgatcgca aattgaattt cccgcgcggt ggtcgtcgtc ctggacagca
     1081 gccccgcgac gccggtggat gttcccgtcc catcgaaaat gtccgtgttg tcgggcgtgg
     1141 tgggcggagc aaagttggcg tgattgaaaa ggttaaagaa ctcagcccgg aactggatgt
     1201 tgaagttctc cgaaatcttt ctgatgtagt tgtttttgaa cacggagaag tccacctcgg
     1261 tgattccggg accgaccagg atattccggc cggcattgcc gcgaagattg aaacagggca
     1321 gggccgggag ctgcggttgc ggggtaaagc ccggggcggg attcgcggga tttagggggt
     1381 cgaatgtctg tccgggtagc agccccaggc tcagtggcac tggatcgcag ttcgcgttcc
     1441 aaaaagccaa gttcggtgca gtaggcacag agaagcactg ggtcttgatg taattgttgg
     1501 ggttcccggg attcgtgagc gacttgcatc ccgggccgcc agtaagtttg atgtaagccc
     1561 aatcgtcgtt gttcagcgaa ttcgcggggt ccgatcccgt gccccaagtg gcggtgaacg
     1621 gcactccatc gctggctgtg aagatcagcc ccagttgcca gccatccgtg atccatctgg
     1681 ccggtccggt aaacgatttg gcagtaggaa tctgccaagt cccactcagg accagcgtgc
     1741 gcccgatgtt gtaatccgct acgccgcggc ccaccttggg gtcgaagtaa ttgaggctgg
     1801 agatcgaatt gccgaacgcg tcgccggcaa ccgtcgccga actggtgtcg atgctcttcc
     1861 cccaggtgta gacgccctgc atctgaaacc catggctcat gcgcttggac acctgcaatt
     1921 ccatggcatc gtagtaggag cgtccctgat aaaacattcc gcgcactgaa ttgaatgtcg
     1981 ggttgaaggg ctcgggtgcc agcccgacat tcggtccact ggtgaggttt ccggctgcat
     2041 ccacttgcgg ccagagatat ccttgcggcg tcaatgtcgg gaggatctga ttggagtcat
     2101 ccacgcggaa gggttgatgc aggccgcgcg agcccacgta ggcaaccata gccgacagat
     2161 tcggcgtgag ctggtactgg acattcaagt tccactgcat gacgtagttc cgcttggggt
     2221 tgggttcgac aaatgtggtg cgcaatcgac tcgacggcag cgtcgagggc aggccgctga
     2281 aaaacggcgt ggtcgaagta aagcatgtag tgcatccagg agcgggagga tccttaacaa
     2341 tagtatattg aaaaaacgga gccgccaggg tggtcaaaag tatgtactgg tagggcagcg
     2401 gcaatacgtc aaacatcccg tatcccccgc ggaccgccaa tttcccattg tgcagcgggt
     2461 cccaggcaaa accgacgcgc ggttcaaaat tcttcagagt gggattactg aagaagggat
     2521 cgcccaggtg agccgttgcg tcggtgagct tcctcaagtt ggcgagtttg ccgttggctt
     2581 ccgttggcac ggtggacatc tcgtagcgca gacccaggtt cagggtgaag ttgggccgca
     2641 cgcgccaatc gtcctgcaca taggcgccga aaagattcgt gcgcaggtgc cgcggggtaa
     2701 ccgtgctgac gactcctcct tgaattctcc ggggattgtt ggtcagaaaa ttagtcagat
     2761 tcttgaactg ccagattccg ttcgggtccg agaaggcccg gatctcgagc agcatgcgct
     2821 cgacgacgcc gccgaatttg atggtgtgcg ttcctctgct gagaaaggcg tcgtcgtaca
     2881 actggaatga attccagttg tagtagtaag tagggaggct gccgactccg ccgggcaagg
     2941 gagaaagacc actgctgatc tggacttgcg ccgcggtccg gccagcgaac gaggccagcg
     3001 tgggatcctt cgccgccgga tttatcgcgc tgacgccctg attgttgttg acggcggcat
     3061 gattaaaccc gaaacgaatc gaattcacga ggcccgggtt gaacgtgtgt gtttcctcca
     3121 ccacagcgat ctgtctcgaa gtcagactgc ccagcattac gttgtcgaac gagtcgggag
     3181 ccgagtacgg agtccgatcg aacacgtagg tgccgaacag gctgtcttta tcggtgaatt
     3241 tatggtcgat gcgcgcggtc aagaagtttt cgttcaccac ctgctgaccg gcgaaagtga
     3301 attgggcgtt gtcgggattc gacgtcacgg ggccgtttgg cttggggtac aaggggaagt
     3361 aaagcaaagc actattatgg atacacacgt tggactgccc aggcaccaat agtgtggtat
     3421 tggggatggc ggcgcaagag gccaccggag gattcccgtt ggcgaggatt ccgttgcggg
     3481 cgttcaaact cggcaccgta agcagggttg aaatgccttt gccctggcgg acaccttcgt
     3541 agtcggcgaa aaagaacgtg tgatccttaa cgatgggccc gccgactgag ccgccaaact
     3601 ggttgcgctt gaaggccggg atggttccgg catcgaagaa atttctggca tccagagcgc
     3661 tattgcgcag gaactcgtag gcgctgccat gaaacgaatt cgcgcccgag cgagtgacgg
     3721 cattgacgac cccgccggaa gtttttccat actcggccga atagttgctg gtcagcacgg
     3781 agaattcctg aatggcgtcg acgccaagat tgccccccag cacgctgccg ggcccgccgt
     3841 tggcgtaatc attcaggctg gcaccgtcga gccggtagtt gttctgctgc ggccgggcgc
     3901 cggaaatggt caactgttgc ccgaaaccgc gattgccgcg gtctgcgccc acggcgaagg
     3961 aaggctgggt ctggatggca tccacaccgg cttgaagggt ggccagatcc gtccaactgc
     4021 ggccgttcag aggcagctcg cggaccgtcg tggcgttgac cgtggcgctg atgtccgagg
     4081 tggaaagctg cacggtggga gcttcggtgg tcacctcaac cgtcttggta atcgtgccga
     4141 cctgcatggt cagattcaaa gtttgtagtc cgcccaccgt caactcgatg ccgcgcttca
     4201 cctcggtgtt gaatccttgc gccgaaatcg tcacttcgta actgcccggc agaaggtttg
     4261 acgcggtgta gtagccgtcg gaattcgtgg tgacgctccg cgcaataccc gtggcaatgt
     4321 tcttgatggt gactttggcg ttgggaatgg ctccgccgct gctatcggtg atcgtccccg
     4381 agatcgtccc acccaccacc tgggcgtgaa cgggaagggg cgcagacaca ccgcagatga
     4441 gcgccagagc gagaagaccg gcaccaaacg atttcaaaat agacttgagc gtcatggagc
     4501 acccccttct gagcctctga acgcagaggt taactcaatc tgccccgcaa agaatgaaaa
     4561 taacattaat taaaatttat ctatgtgccg cgacttcctg cgcgatcgac ggagttgatt
     4621 aaccacaggg taaattcgcg agtcccgtca gcaggaacag cgcaaacttg cggagccggt
     4681 tggaaggacg ttgcctggga tgaagaataa tcatgaagca ccgacgggaa ttgtttcccc
     4741 atcgcgcagc gcgacgctcc ctcctctcgt ttgtccttgc ctggaaggaa gtttaccgtg
     4801 gtctagttca gtgaagaacc aaagtattga cccgcttggg cacttctccc acggggatga
     4861 gagccacttc tttcagagtc ttggtgtcaa tggcggacac cgacctcgct gcggagtctg
     4921 aaacgtaggc gaacttgctg tccggcgtga acgtgatcca ctccggcacc gccccggtag
     4981 gcgaaagtcc caacgggtgc acctcggaca gcgcaacgtg tccgatcaac ttaagatcgg
     5041 gcagggagta cacgaaaacg gcgtttgcca gggtgctgtt gacccatagt gattttccat
     5101 cgggcgcgac tccgatgccg tgagacggag tgccggcgcg cccctcaaca attccgaatc
     5161 cgccgggttg ctccggcagc ttgatgcgcg ccacctccgc gcgcttcgcg aaatccacga
     5221 cggcaaatcc gtggtagttg gaaagctgga cgaagatgcg gctggtggac ccgtcgggat
     5281 tggtctcaaa agccatgggg cgcactccgg cttcgaattt gacctcccac acagcttgat
     5341 cggtctggag atcgacgacc gtggccatca tggattctat cgaaccgctg accgcatact
     5401 tgccgtccgg tgtgacgtag acgttgtgca cgccgccgtt cacggggatg cttttcaccc
     5461 gctgaaggga cgttgtgtcg atcacatcga ggacggcagg tccaaaccga atgcccacca
     5521 gcacgcggcc tccatccttg gtgatggcca ggttgttggg gcggccgctg agagctattt
     5581 tccgttgtat ctctccgctc ttccgatcca ccacatcgag cacgctctcc gattcattgc
     5641 tgatgtagag gcgcgtccca tcgggagaaa aattgacgcc gtgcgggagc tcgatgcctc
     5701 gtatcacctg gacgactttg ttcgtcgcgg gatcaacaac gtccaccgta tcgccggcgc
     5761 tgttggtaac gtagatgcgc gcgatgggca cgggcgaaga ttccgcaacg gctcgcgtcg
     5821 tggaactgcg cacccactcc gccaggacca gccagtcagg gtcgttctga gactgaaatt
     5881 gcctgcctcc ggaatgaaac ggatccccac ccgcttcggg tgccagcgca tgcatcaaga
     5941 gcctgctgga cgtgggatct ccggggacca cctgctgcaa agcgttctgg aagttgcgct
     6001 gcgattgctc gtccgtccaa tccgctcttc ccgctgacag tgtcgcgaga cgcaagagcc
     6061 ggttggcttc cgtatggcaa acatagcagc gggcgtgacc cggccgctcc ttcaagaaaa
     6121 taggctcgac cctggtcttg aagatttcga gatcgagagc atgcgttgcg gtggaaggag
     6181 cggccggctc cacggcttcc ggcgccggag agctcttgcg gacccaatcg gcaagcattt
     6241 gccattccgg gtcgctctgc gacgcccaga atttgccgcc ggtgtgcatg ggatcaccgc
     6301 ccgcctcttg ggcaagggcg tgaagcagga gcgggctttt cagcggattg gaaggagtga
     6361 ccaactgccc gacggcggcg aagtttcgtc gtgactgctc ctcggtccag gaggagcttc
     6421 ccgcggacaa ccgctccagc cgcaggcgcg tgggcaagac ggaatggcag tcgtagcagc
     6481 tcacgcccgc ttgccgcttc ttgaggaaaa ttggctcgat acggctgcga tacgcctgat
     6541 agtcgagcga cggctgtgcc tgtgctgcgc ctccggcaga ctgctcggaa ctcgccagca
     6601 gcgcccgggg cgcagtgacc gccgccgcag ccggcccttt cgtcccggaa gaaaggttat
     6661 tgaccgcggc aaccaggaac agcagcaacg tcgctttctt ttttttcaac atgtcggcgt
     6721 ttccgtgttt caaagattcc actcatcgta ttcggaaggc tcggtcatcg ggaagctccc
     6781 cgcttttctc gaccataaac cagcaggcga aacgttccgg gaatcggggg ccgtgccggg
     6841 tggggcttct ccgcagtcgg ggccggtgcc ggtccaaaat ccgccgtgtc gatgaaaagc
     6901 tggtgcgttt ttggatcgag ggccatgttg cgcgccccga actcggtctg cacggtctcg
     6961 atcacgctga acttgtcggg cgtgtcttcg tgaaagacat gcaattcgcc ttcgcgcaca
     7021 gcggtgaaca gcatgcctgt ctttggctca aagatgttgg tatccacacc ttcaccgatc
     7081 ggaaatgtct ggaggacctt gccgttgtcg gcgtccatga tcgctagaat cttgttacgg
     7141 cctccgatga acagacgccg gtgctgtacg tccatgtcca tggcggtggc ctctgcggcc
     7201 ggagcgatcg gccaccgcgc cttgatggtg agcgcgcggg aatcgagcgc cacgacctcg
     7261 ttcttgtcct cgatattgtc gtagatcatt cccttgccgt cggccacggc gtattccgga
     7321 cggccgccca tgggtaccgt ccccaccagt gttccgctca cgggatcgat caccgacgca
     7381 tcgcccgagc ggccgttcat ggtgaagacg tgcttcgagg ccggatcgta aatgatgcag
     7441 tccgggttgc caccggtctt cacctggccg acaaccttca gagtcttcag gtcgaaaatg
     7501 acggcctggt cggcgccgcc gtcgctgatg aatcccttgc caacctcttt gaccaggacg
     7561 atcccgtgca cccgtttcaa atcaacaatg ctgcccaaca cggcgccgct gtcggcatcg
     7621 accaccttca cctcggtgcc gtgcgagatg tagagacggc gcgtggaagc atcgaacgta
     7681 atgtagtccc aatactccgt cccgcccggc gccgcgccga gtggatattt cgtgagcagg
     7741 tgatagccgc tattcggcgt accggccagg gcgaatgaac cggccagcag aatcgcagcg
     7801 aacatcgcga ggcggcgaag ttctgaacgc ttcatggaaa ccctccgttc gatcgaattc
     7861 tcggaccagc ggaatcgccg acgggagcgc gaatctccgg ccgctcactt tctgggataa
     7921 tcgaattggg aatcgcccgc gagaatcttt tgcatttgag cgttcgcgta cttccacgga
     7981 tcacgcgagt attcttcata attacccggt acattgatcc ccgggatgtc cgggatccag
     8041 tcttcggcaa gcaatctgcc gggatcgccg tactttgcgg ccaatgcgcg cacctcgggg
     8101 tcatccaggg cggtcaaatg cccgcggtcc acgatggtca cccatttgcc ggaagtccgc
     8161 cggtgcacct tgtaggtgat gaaataattc tgaatgtgaa agtcgtgacc gttgggaaga
     8221 tcgagctctt cggcaaattt ctgccacttc tttggcgtgc ccagggaacc gggatcgtgg
     8281 cccagctcat ttccaaaccc ccaatgaaat gcgccgctgc ggtagcgctc ataggtcagg
     8341 caatagactc ccgccgtgcc tccgccgtag aagtcggtgg gattccgaaa gtacttggga
     8401 ttggtgccta ccgcaacctc ccatagccgc cagtacccgg gagtgtcgta attggggtac
     8461 gtagcgtcat tgatgtgcgg gtagtgttgg aactcgcgga tgacgtcgcc gtagcggcct
     8521 ccgcccttca cgtccgtgat gtggccatcc tggagatgaa tctccatgtg cggccagaag
     8581 ccgcccgagc cgttggtgcc ggcgatcact ccgctggctc tggcggtggg cgaacgcggt
     8641 atccaatccg gaaaagtttt tggatagttg tcaaagtcct ggccgtactg gccggtcgct
     8701 gaatcgggat acatcagcac gtgaccgcgc cagtagatcc cgcgcctcca gcgttgcgcc
     8761 atttcttccg ggacgtccca ggtgacgttg gtgccttccg gatcggtcac cacaacctta
     8821 tccacatccg gagtcaccat ggcgatggtt ttcttctcaa tgaactgcca cacctctgcc
     8881 ggaaaagatg aaacctggct caccacttcc caactgttgg tgaatgagca gcgccccagg
     8941 tacttccctt ccagcggagc catggtgcgc gcatagaagg cgccgccggg atgaccccaa
     9001 tagacacctt tgattttcgg atgctgttgg agataggttc ggaccgctgc acccacgctc
     9061 tcgttggtca gttttttcca ctggatttgc agctcaggcg tcatctcgtc gcgcgtgggg
     9121 tagagggcat cgtacaggcc gggatttttc tgtcgcagcc attccttcgg gacctccggg
     9181 tgcgtgaaga cgcgctcgat ccagtagtac ttcgcctcca tgaaaccttc ttcggcgctg
     9241 cggatttccg tcttcagcct gatcttctcc gcatcttcct tggaaactcc caccaaagaa
     9301 taattcggca cgatgtccgc atgaacaccg cgctcggcga gggcgcgcac gatcgcctgg
     9361 acgaccaggt cttccgccgt ggtgtccggc acgatcatga tttcttcgcc cggctggaga
     9421 agaccgaggc ccaaccccgg agaatttccg ccgaaacccg ctttgttgcg caccaagtcg
     9481 cgcgcgtggg gcatcatgtc gtcgattgtc gtcagcttct tcaagtagga aggccaacgg
     9541 ggtaaagggt aggccggagg ttttgcggtt gattgcctcc ctcgcgcctg cggactcatc
     9601 cccagcagcg cgattcccag gagcgcaagg ggtgccagca acagcggctt ggtgttccaa
     9661 tatctcacaa agctgtctcc caatttgtgg actcgagccc gcgaacacag ccgctgcgtc
     9721 cttcccggaa ccggacccgt tctcgccctt cgatgtccac ttgtctgggc gaaatcctac
     9781 tgcggcatcg ggatgaagtt caccttaagt tttctttagg ttgggcggcg aaattcgcgt
     9841 ggcgcggtgg atggagaact cggccggaat cgtagtgctg acttccgggc gaggccttag
     9901 ctcagaatca gcttctgttt aagttcactc tcgcggatgg attcgatggg gcgcgaatcg
     9961 ggcagaatca cggtgaaggc tgaaccggcg cccagcgtgc tctcgcactc aatcgagccg
    10021 ccctgggcgc gcgcgatggc ctgggcaatg gccaggccca gaccgccgct atgggcttca
    10081 ctgttgccgg aaggcgccgc gcggtaaaat cgctcaaaga tgggagccag atgctcgctg
    10141 gagatcccga tgccctggtc ttcgaagcgg acccggatca cagagtcacg ccgctggaga
    10201 gttacgcgca cgcgggtatc cgccagggag tacttgatgg cgttatcgag aaagatcaga
    10261 aacaattggt gaaggaaagc ctcgtctcca aaacatgcca tttcctggtt tagttccctg
    10321 acgatgacga tattcttcgc ccgcgcgcgc gaggccatca gcgcgcaagc ctcttccagg
    10381 acttcattga tgtagagcgg ctcggattcc agacggagct gcccggcgtc ggccatggaa
    10441 agcgtgaaca gtccttccac aatgcgcgtg aacttcttca gctcgtcgtc aatcacgcag
    10501 agagttttcc gatattcctc ggcgctgcgg ggtctggaaa gcaacagctc cgtctccccg
    10561 tggagaacgg cgaggggcgt gcggagttca tgcgaggcgt cggtcacgaa ctgacgcaac
    10621 tggcgcaggg ccgagtcgac gctttgcagg agctgattaa atgtctcggc cagacgaccc
    10681 agctcgtcgt gttgggaggt taccgccatg ggtctgccga atccttcccc gttggccacc
    10741 ttcctggcca tcagagcggc ctgctcggtg agcgcagtga tcggtgccag gctcctgccc
    10801 acgtaaaagg cggagattcc cgcggtgagc agaaggctca atggaaagag gaacagagcg
    10861 atgcccccga aactgtcgag cacgcggttg gtgccgaacg tcgggatggc tacggccagg
    10921 atgcgccgct gtccgccctg ctcgaatgga ataagcgtca cgcgcaccgg ttcgccattc
    10981 tcgatggcgg cgatcgcgaa gacgggttct gaagccgcgg ggttgatgcc cttcaaatca
    11041 aatggagcgg ggagattttt cgatcgttgc agcacgcggc cggcggggtc cagcagctcg
    11101 aaaaattcgc cgggaaggtc cagttggttc acgttgtcct gatccggctt ggcgatcaga
    11161 tgggcaatca ccggacgggc agcggcctcc aactggcgat tgaggtgagt atggacaacc
    11221 cgcgcaagga gagcccagaa ggcaagatag gaaaccgcaa gcaacactcc caccacggtg
    11281 cagaacagca ccatcatccg gaaacgcagc gactccgcct tcctcattcc ggcatcacca
    11341 gttgatagcc cactccgcgc acggtatgaa tcagcttggc cggccgattc tggtcgatct
    11401 tctggcgcag gcggttgatg tagacttcga cgatgttggt ctcgctgtca aagtggtagt
    11461 tccagatgtg ggcgatgatt tcgctgcggg taatgggctc cggagcgcgc agcagaaaga
    11521 gctcgaggag agcgaattct ttggacgtca gtagaatggc ttccttgccc cgggtggccc
    11581 ggcgacggtg acggtcgagc tccaggtcgg catagcagag ttttgcgccg cccttgtgga
    11641 accctcgccg gaccagtgcg cgcacgcgcg ccaggagttc cgccagagca aaaggcttgg
    11701 tgagatagtc gtccgcgccg gcgtccaggc cctccactct ctgctcgaca agagttcggg
    11761 ccgtaagcat cagaattggc gcctcgattc cctgctggcg cagctcacgg cagacttcga
    11821 tgccgctgag gccgggcaat ctcacgtcga gaagaatggc atcgaaggtg acggccgtgg
    11881 ccatctccaa agctttctcg cccgtttccg ccacatcgac tgcgtaggaa ttctcccgca
    11941 gtgcgcgcgc aatgaaactg gcgaccctac gttcgtcttc caccaagaga atgcgcatac
    12001 tgtgccgccc gattaaactt atacaacgat cgaacaaaac tgcgcgagtg aaaatcttcc
    12061 aggctacgcc ctcggagggg cacgcgggcc ctgggtccaa tcgttcctcg ccgacccacg
    12121 ccggtggtca cttcaggtcg ctgcggacga cttgtcctcc cttcatgacg aacttgaccc
    12181 gttcgagctc cgtgacgtcc ttcagcggat ctcctgagac ggcgatgata tccgcatact
    12241 tgcctttctc aatcgagccg attcggtcct gccagcccat catctccgcc gccaccgtgg
    12301 ttgcggactg gatggcctgc gcgggtgtca tgccgtactt gaccaggtat tcgaactcga
    12361 cggcttgaga accgtgagga aagggcccca cgccggtgcc gaatgagatc tttacgccgc
    12421 gggcgatcgc caatctcccg ctcttttctc gcaaggcttc gaggctgtac tttccgccgg
    12481 tggccttggc gtcccgcacg gtatagtcgg cgagagagta gtgataggcg gtcagctcga
    12541 gatagattcc cttctgcttc atcatgtccg cggtagcctc gtcgagctgg agagcatgct
    12601 cgatcgtgtc ggatcccgcc tccacgcagt tgcgcaagcc ctcgcctcca aacgcatggc
    12661 aggcggcttt ctttccgtga cgatgggcct catccacgat cgccttgacc tcctccaaag
    12721 tgaaggtcgg gtcactccag agttttccgt ccggctcaaa ctcgtactcg gccgtggagt
    12781 ggaacttgat ccaatcggct ccaaagcgaa tctgttcgcg aaccgcctgg cgcgccgccc
    12841 aaggactgtc caccgtctgg tagttccgcg gcaagctcac ttccggcgag cccaaaattc
    12901 cctctccggt ggcgacaaga cccatcgtcg acacctgcat ccgcgggccc tgaaaaaagc
    12961 cgcggttgat ggccttcttg atgtccacgt cggcatagcc gccgccgtgg ctcatcaaat
    13021 cgcgcaacgt ggtgaacccc gcgcggaggt ccttctgggc gttggccagg gccagcagcg
    13081 tccgatactc acgggtatcg ttaatattgc tggcaaacgg cggaccgccg ggcttgatcc
    13141 cgtcaagccc gaagccgaag acatgcgtat gaccatcaat caaaccgggc aacacggtgg
    13201 cctggccgag gtcgatcact tgcgccccgg ccgggatttg gacttggccg ctgggaccca
    13261 cagcggtgat cttctccccc tcaacgagca cgacttggtt tgtcagcgtc tggcccgact
    13321 tgctgtcaaa caatcgtccg gcgcggatgg ccaccaccgc gggagcctgt gcatgcagcg
    13381 cagtcgcgag tcccatcagc aaaaatagca tcgccgattt tgaattccag gacttcaaga
    13441 gagcctccgt cgaaccgtag tttcatagca agaccatcgg tttccgattc gcgcctcgga
    13501 aacggagctg gatagcggac caaaacttca gacctcccca ggactccttc gagccggctg
    13561 gggccgcatc cagaattgaa aggcgcgtta tcgctggacg tcgaagacca atacctcagc
    13621 attcgtggtt ccgtgttgcg gcacggccac aaaatacttg cccagctccg gcaccagccg
    13681 ggcggttctc ccgacgggcc ccgtggtcac ctttccaagg tgtttgtaat tgttggcgtc
    13741 ggtctggccg tacacatcca ctgcgccgtc cgcagaggca taaaaccgtt tgctggccgg
    13801 gtcgaaaacg acatcgtcaa cccccgtggt gatggccaac gcggcaacct cctttcccga
    13861 ctcggtgtcc agcacggcaa cctgcccatc gcggcaggcc gcgaacaagc gatggttcgc
    13921 ttcatcgagg gccatggcca cgttgacctt gcactttgtg accggccagg tggcgacgac
    13981 ttcgcgtttt gcacggtcca ccaccgcgat cgagttcttg gttctgatgt tgacatagat
    14041 tctcgcgctc gatttttcga gcgtcatggc ctcaatcgtt tccccgtcga ccttgatgtc
    14101 cgcaagcttc gtccccgccg tcgtatccac gacgctgagc atggagtacg tctggtggac
    14161 atccccgccg ccgttgtcga tgtaaagaaa ttttgtggcg gaatcgtatc caatggagtc
    14221 agcgtctgcc aggagcttca cgctcttgat caggccgtac gtcttgccgt caaaaatctt
    14281 cacgtctccc gcctggccat cggtgacgta gatgcggttc aggtcttcgc gatacaacac
    14341 ggcgtgcggt ctttcgatcc cggttatggt gtggaccaat ttcccggtct tcaggtcaaa
    14401 caccaccacc gccttgtaat cttccggggt ggtgaacagc cggttcccct tcagatcgac
    14461 ttcgaagtgg tcgaaatgcc ctttgactgt ggcagggaac gtaaaggtct ggacctgttt
    14521 gagtggcgcg gtctgctgac attgcgcggg gaccgcgaat aggacgaggg ccgtcatgag
    14581 gagcaaatac tgttttttca tcttttctcc ctaagcgttt atgttctgtt ctcttcccgg
    14641 gtatttcatc aagcttccgc cccgtcccgc ccgcggcatg gacccggctc gggcgggggc
    14701 ggagaggcag caagaccccg gcggcaagcc agcgcgccag agcatagctc acctctcagc
    14761 aagcgagctt aaaacaagat taatttgcat ttagctgaag aattactgaa gagattttaa
    14821 ggtggcgaca ggctggtcca ctccgaggcc aacgcagaaa aatttccatg gactgtcgaa
    14881 tccttcgttg actcgaatca ggcggtggcg tagcatccgc ggcgtcgaag ccgttttttt
    14941 cagccaagct ttgggcagct tttctttctc tctccatccg gtgagagcga tcgaataacg
    15001 ggagcaagta aaattgcctg atcgaagccc tacgcgtgtt cgctggcaca ttctcgccat
    15061 cctgacgctc atcatggtgg ccacggcgct cggtcgcctg aatcttggga tcgccggaaa
    15121 gtacatgcag gaggaatttc agttcacaac gcagacgatg ggatggatcc tcggagcctt
    15181 cgccttcggc tatgcgctct ttcaagtccc ttgtggatgg gccggcgatc gtttcggtcc
    15241 gcgcatcacg cttaccctgg cgatcctgtg gtgggccaca ttcacgatgt tcatgagttt
    15301 ggttccttat ctccgcggaa gctcatggtt gaatcttgcc tgggcatttg gactggtcag
    15361 gtttctaacc ggggccggcg aggccgcgag ctaccccaac gccaacaaga tcgtcgcttt
    15421 ctggacggcg aaggcggaac gcggtatcgg cagcagcttg ttgcttggcg gggtcggcgc
    15481 cggcggcgtc ctcgcgccca tcttgtttgc tggcatcatg cagcgctggg gctggcggtc
    15541 ctcctttgtg gtgtccggcg tgcttgccgc ggttgtagcc gcgatctggt tctttttctc
    15601 gaccaaccgg cccgaagagc acccgcgcgt caactccgag gaactcgaga ttctcggatc
    15661 aacgctgcgc ggccacggca tccattcgtt ccggttgaag ggcactccat ggcgtcagat
    15721 tttctccagc cgctcggtct gggccttgat cctcagttat ttttttcacg gttacacgcc
    15781 gtacatttat ttcgcctggt tcttcattta tctcacgcgc gtgcgcggcc ttaccgtcac
    15841 ccagggtgga ctgtggggtt ccactccctt cctggccatg accctgatgg ccccgctcgg
    15901 aggttggctg tccgacaaag ccgtggtccg tttcggccgg cgccgcggac ggcagagcac
    15961 ggtgtgggta ggaatgacct gttcggcact gttcctatgg gcgggaagtc atgcgctcaa
    16021 caacacctcc gccattctgc tgctggccac ggctgcggga ttcagcaatt tcgccgcacc
    16081 aagctggtgg gccacctgca tcgatttggc gcccaaccac tcgggatctc tttccggtct
    16141 gatgaacacg tgcgccaata ttgccggagg gatctcgccg atcctgacgg cacgcatcgc
    16201 cacgcaattc ggctggacga aagcgctcga ttttgccgca ctggtgagtt tcacggctgg
    16261 catcatatgg gtcttcgtaa acgccagcga caatctggag gctggcgcca gcgtcgaggc
    16321 gccatcgtga ttcacgaagc ggtatcggcg gccgcacagg ccgcgtccct tctcggccgt
    16381 aacctcttcc aaggagatgg cttgtgaaaa gaatgaccac agccttccgc gagatgctaa
    16441 aggctcctgg aattatccat gcgccgatcg cctatgaccc gctgaccgcc aggatcgccg
    16501 agcgggtggg attccgctgt ttggacttgg gtggttacgc gctgggcgcg agctcctgtg
    16561 ttgcggaacc gcttctcagt ctggaggaag tcctgagggc cacgcggcgc atcaccgccg
    16621 ccgttgcgat tcccttgatg gtggacggcg gcgccggtta tggcgagccg gctcacgtga
    16681 ttcgcacggt gcaagaactg gaacgcgccg gcgccgctgc cgttcacctt gaggaccagg
    16741 tctacccaaa acgaattcat tatcacaaag gcgttgagca cgtaatttca gccggcgaga
    16801 tgtgtcaaaa gattcgatgc gcggtgcgag ctcggcggga tcctgacttc gtcatcgctg
    16861 cgcgcaccga cgccatgcgc accgacggct tcgccgaggg cgttcgccgc gccaacctct
    16921 acgcggaagc cggagcggac atggtcatga ttttccccaa cacctcagaa gaggcccgca
    16981 aggggccggc ggaagtccgc gcgcctctga tctacgtcaa cagcgaagga aaccggctgg
    17041 gaaggcccat ctttgcggcc ggagaattgg aggccatggg atacaagatg ttgaatgacg
    17101 ccatcagcgc catcgcggtc atgttcaaga gcgtaagcga actgtttttg cgtctcaaag
    17161 aaacgggccg taccggcatg gaccaggcgg tatttgtgaa gattcgcaaa gagattgaag
    17221 acaccatagg cctggaggag tactaccgga tcgaggaaga aacagtggag aaccgggagt
    17281 agagggactc cgtccccaca tctatcggtt catcttcaac atggagagtc aacagcaatc
    17341 attcgatggg aggtatggac acaacatgag ttgttctcac caaccacgca aacactttcg
    17401 ctgccccgat aaacatcctt cgtcagctat ggcgcctccg ccgagcagga tcaatctgtt
    17461 tttccaccca tccgctctct tgcgatttgt tttggtgtgc gcgatttcca tactgccgtc
    17521 acccccggca gccgcacaac tctctcccgg gaacgggccg gcagcacggc aagattctgt
    17581 gcagcgcgtc atactgctga gcatcgacgg catgcacagc ctggatctcg cgaatctgat
    17641 taaggagaga cccgagtcga ctctcgcaaa attgagccag cacggggtca cctattcgaa
    17701 tgcttccacc tcatttccat ccaattcatg gccgggactg ctttccatgg tgaccggcgg
    17761 gtctcccaac gttacaggag ttatttttga gaactcctac gaccgcagcc tctcgcctcc
    17821 cggttcggat tgcaccaaga taggaactgc cataattttt gattcctcaa tcgataaaga
    17881 tcgtgatgcg ctcgatggcg gaggaggcat cgatccgaat aagctgccgc gcgatccgaa
    17941 gaagggatgc gctcctgtct ttccgcacag cttcatccgc gtaaatacga ttttcgaagt
    18001 catcaagaaa agcggccgcc gcacggcatg gtcggacaag catcccgcct acgaatttgt
    18061 gaacgggccc tcgggcaccg gggtggatga cctttttgcg cccgagattc gcgcttctca
    18121 cgccaccagg agcgttgaaa aagcaaaagc gtatgacgac tccaaagtgg cggccatcct
    18181 gagtgaaatc gacggcaagg accacaccgg agcgagccag gtgggcgtac cggcaatttt
    18241 cggcatgaat ttccaggcca tcagcgttgc gcaaaagacg accggaaatg gctatatcga
    18301 cggtagcggg agtccaagcc cagctttgca tgacgccttc actcacacgg atcagtccat
    18361 cggaaaaatg atggccaggc ttcgggagcg cggcttgttc gactccaccc tggtcatcat
    18421 cactgccaag cacgccgaca gcccgattga tcccgcaaaa ttaaaactcg ccgacctggg
    18481 acttattccg aaaaccgtcg ccgccatcgg caaaggctta ttggcgggtg tagagcagga
    18541 tggaagcgtt gccatgctct ggctttcgga tcaggaacga acggcggatg tcgtcaatgc
    18601 gttgcgaaaa atccaggcgg aggccggc
//
LOCUS       DASYVO010000348         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_178339, whole genome shotgun sequence.
ACCESSION   DASYVO010000348 DASYVO010000000
VERSION     DASYVO010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178339"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..2440
                     /gene="tkt"
                     /locus_tag="VGR03_11845"
     CDS             386..2440
                     /gene="tkt"
                     /locus_tag="VGR03_11845"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1995014.1"
                     /translation="MSESNLDQLSINTIRTLAIDAVQQANSGHPGAPMGLAPVVYSLW
                     QRFLRYDPTDPTWPNRDRFVLSAGHASMLLYATLHLAGVRSVNAKGEVSNDLAVSMEE
                     IKRFRQIDSKTPGHPESHITSGVETTTGPLGQGAGNSVGMAIAGKWLAENFNRAGFDI
                     FNFNVYAICGDGDLMEGVACEAASLAGHLKLSNLCWIYDNNRVTLDGPAEWSFSEDVM
                     TRFKGYGWNVTHVTDANDLNMLARGYDNFLRSKDHPTLIVVDSHIGYGSPHKQDSYEA
                     HGEPLGEAEVKLVKKNYGWPEDAKFLVPDGVYDQFKNGVGKRGAEARAAWKAKFEEYK
                     KQFPQQVEQLNRMQSGQLPDGWDKDLPSFPADPKGMATRESSGKTLNALAKNIPWLVG
                     GSADLAKSNKTNLTFDGAGDFYPNQYRGRNVHFGVREHAMGAIVNGMTLSKLRAFSAT
                     FFNFSDYMRPSMRLGALMEIPAMYIFTHDSIGVGEDGPTHQPIEQLASLRAMPNMLVL
                     RPGDANEVVEAYKVILQHTHGPSTLVLTRQAMPTFDRTKYASAAGVAKGAYILADAPG
                     GKPDVILMGSGSEVSLCMEAREKLNAAGIKARVVSMPSWELFERQDAAYKESVLPASV
                     SARVSVEMASTFGWERYVGLNGKMIGMRSFGASAPLKDLLKKFGIEVDKVVAVAREVL
                     GK"
BASE COUNT          577 a          721 c          786 g          521 t
ORIGIN      
        1 ttccgccacc cgggcagggc agatacagaa aagtagcatg tacggcggct gggctcaacc
       61 gggacaaagg cggaaacacc gggacacgtt ttctccgttc cgtcacttca agctgttaat
      121 ccctcgtgtg aatacgccgt aacgtcgttg acaagccgtg catggcaaga tacgtttgga
      181 ttctcttcca atacgctagc cggtctgagg ctgttcttga aaaaaaccag atggggcggt
      241 ggggcggttc ctgggaaatg ggtcgaagcg cgggcgagcg agtgctttca ttttgtcgta
      301 aaatcccttc atccgatccg taatgcggta catctaaaat ctgacgaaaa ccagcgggcg
      361 gcttccctcc cgcactggag ccgacgtgag cgagtcgaac ctcgaccagc tgagcatcaa
      421 cactatccgt actctggcga tcgatgcggt ccagcaggcc aattccggcc atcccggcgc
      481 cccgatgggt cttgcgccgg tggtctactc tctgtggcaa cgcttcctac gctacgatcc
      541 cacggatccc acctggccaa atcgtgaccg ttttgttctg tccgcgggtc acgcttcgat
      601 gctgctctac gccacgctgc atctcgcggg cgtgcgctct gtcaacgcca aaggggaagt
      661 gtcgaacgat ctcgccgttt cgatggagga gatcaagcgc ttccggcaaa tcgacagcaa
      721 aaccccggga catcctgagt cgcacataac ttctggtgtg gaaaccacga cggggccgct
      781 ggggcagggt gcgggaaaca gcgtggggat ggccattgcc ggtaaatggc tggcggaaaa
      841 tttcaatcgc gccggcttcg acattttcaa tttcaatgtc tacgccatct gcggagatgg
      901 cgatcttatg gagggcgtag cttgcgaagc tgcttcgctc gcgggccatc tgaagctttc
      961 caacctctgc tggatctacg acaacaaccg ggtgaccctc gatggtcccg ccgaatggtc
     1021 cttcagtgag gatgtgatga cgcgattcaa aggctacggc tggaacgtta cgcacgtcac
     1081 agacgcgaac gatttgaata tgctggcccg cggctacgat aattttctca ggtcgaaaga
     1141 ccatccgacg ctcatcgtgg ttgacagcca cattggatat ggatcgcccc acaagcagga
     1201 ttcctacgaa gcacatggcg agccgctggg cgaggcggaa gtcaaactcg taaagaaaaa
     1261 ttacgggtgg cccgaagatg caaagtttct ggtccccgat ggagtctacg atcagttcaa
     1321 aaacggcgtg gggaagcgcg gagccgaggc ccgcgcggcg tggaaggcga aattcgaaga
     1381 gtacaagaaa cagtttcccc agcaagtcga acaactgaac aggatgcaga gcggccaatt
     1441 gccggacggc tgggacaagg atttgccgag cttccctgcc gatccgaagg gtatggcgac
     1501 acgcgaatcc tcgggaaaaa ctctgaacgc cctggcgaag aatatcccct ggctggttgg
     1561 cggatcggca gacctggcca aatccaacaa gacgaatctg accttcgacg gcgcgggaga
     1621 tttctaccca aatcagtatc gcgggcgcaa cgtgcacttc ggcgtgcggg aacatgccat
     1681 gggcgcgatc gtgaatggca tgacgctgtc gaaactccgc gcgttcagcg ccacgttctt
     1741 caatttcagc gattacatgc ggccgagcat gcgcctcggc gcattgatgg aaatcccggc
     1801 aatgtacatt ttcacgcacg actcgattgg cgtgggggaa gacggaccga cgcatcagcc
     1861 catcgagcaa ctagcctcct tgcgcgcgat gcccaacatg ctggttctcc ggccaggcga
     1921 tgccaatgaa gtggtggaag cttacaaggt gatcttgcaa catacccatg ggccctctac
     1981 cttggttctg acgcgccagg ccatgcccac gttcgatcgc acgaaatatg cttccgccgc
     2041 aggagttgcg aagggcgctt acattcttgc cgatgctccg ggcgggaaac ccgatgtgat
     2101 cctcatgggc agcggcagcg aagtatctct ttgcatggaa gcacgcgaaa aattgaatgc
     2161 ggccggcatt aaggcccgag tcgtcagcat gccgtcatgg gaactcttcg aacgccagga
     2221 cgcggcctat aaggagagcg tcctccccgc gagtgtgagc gcgcgggttt ccgtggagat
     2281 ggcttccacg tttggatggg agcgctacgt cggcctcaac ggcaagatga ttggaatgcg
     2341 cagcttcggc gcatcggcgc cgctgaagga cctgctgaaa aaattcggca tcgaagtgga
     2401 caaggtcgtg gcggtggcga gggaagtcct cggaaagtga gccggcgatg aagattgtga
     2461 ttggctcgga ccacgctggg tttcagttga aggtagccat gggcgatttg ctgcgctccc
     2521 tgggacacga tgtcctggat gtcggcgcgt tcaatgacaa tccttccgat tatccggact
     2581 ttgcagaggc cgtgggccga gccgt
//
LOCUS       DASYVO010000349         5996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_38149, whole genome shotgun sequence.
ACCESSION   DASYVO010000349 DASYVO010000000
VERSION     DASYVO010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38149"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VGR03_11850"
     CDS             complement(<1..407)
                     /locus_tag="VGR03_11850"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995015.1"
                     /translation="MIQPDFHEFQRLAKQGNLIPVYDVFSADLLTPVSAYLRIAHGAR
                     YSFLLESVEGGEKIARYTFAGAHPEEIFRYASGACVLESRDRIVWEERDPISFLRERV
                     ERFRPVRIPGLPPLVAGAIGYFSYDMVRLIERIP"
     gene            complement(1022..1867)
                     /locus_tag="VGR03_11855"
     CDS             complement(1022..1867)
                     /locus_tag="VGR03_11855"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEV1995016.1"
                     /translation="MPCAPERSRALASRGSSVPFDRIKDRKVKPDFSQYGFVHQENLI
                     MACIGGSQAHGAKLGATDDTDWYGLYIPPPTKVLGLQREEHFVFTTGGKLGGNGPLDV
                     DVCLYTLMKWAGLAAKGNPSALHFLFAPLEFTTETWDHFSARPEFFLAKGHVKPFLGF
                     ADDQMKRLLGQKGQKNVHRAELEDKHGYDTKYAMHVIRLYGEAKELMEHGRITLPRPN
                     RDELIEIRRGKYSLTEIRQLGAQLESEALAARATSTLPDEVDRDGISRLLADAHLRFW
                     SSSAA"
     gene            complement(1830..3347)
                     /gene="aroE"
                     /locus_tag="VGR03_11860"
     CDS             complement(1830..3347)
                     /gene="aroE"
                     /locus_tag="VGR03_11860"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV1995017.1"
                     /translation="MGLFGTGLRRICAVVAASTAAEMSKLVRVALRQTRTVELRLDWL
                     SSDAERARFLGWLGKDKSRNATFLATCRRREGGGKFAGGVDRELYWLIQAREAGCQWC
                     DLEVETLRKLPGQSVREYPVPPRVMLSVHDFERTPDLPRSMNPPADGGVDAVKIAAEA
                     RSIQDSVRLLRLARSSENFVAVPMGEAGLPARILALREGSALAYAPVAAATAPGQVSL
                     HQMKHLYRAHALTRRTRVYGVIGDPVSHSLSPLLHNTGFAARRVDAVYLPFLVHQLGD
                     FLAAVPEFGVRGFSVTIPHKQAILKHLKECEPLAADIGAVNTVVVRSDGSLYGCNTDY
                     VGVLRALEKKLQIKRSRALIFGAGGSARAAAFALSRAGAIVGICARREKAAKELARAV
                     GGEVVPRRALRTEFFDAILNATPVGMHPHGGISPLAPGELHCRIVMDLIYRPQRTQLL
                     KLAAQKGIATVSGVEMFLAQGFAQWEIWTQKRAPEASMRRAVLGALRAGEKSRPR"
     gene            3515..4390
                     /locus_tag="VGR03_11865"
     CDS             3515..4390
                     /locus_tag="VGR03_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995018.1"
                     /translation="MRARIAVFSLSVFLGTAASSAQNAPQAAPAIPQVDYSTVNCSGF
                     VTDQKVPDEIRLVSAEQSNYKITFARGDYVHINRGQDKGVRVGDRFSVVRPDKDPTDV
                     PWFKWQTKLLKAMGTPYMDLGQIRVVSVQPKVSIAQVNFSCAFMQRGDILQPYQDRPA
                     PPFKDAVAFDHFAPVSGKPVAMMVAGKDYGQVYGKLSAVYVNLGSNQGVKVGDYFRIF
                     RYQGSMAETVPQNKGYQYSMYGFGSAPVRYEWNDLPREVLGEGIVISVSRNSSTMIIT
                     FNSIEVYAGDYIEIE"
     gene            complement(4498..5061)
                     /gene="amrA"
                     /locus_tag="VGR03_11870"
     CDS             complement(4498..5061)
                     /gene="amrA"
                     /locus_tag="VGR03_11870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEV1995019.1"
                     /translation="MPFLSEADRGAALQLARTAVVEAVSHHKLPDVFPREGIFAERRS
                     VFVTLHVRNRLQGCIGVIEANEPLGEAIVRCAASAALEDPRFAPMRTDQLGELSIEIS
                     LLSPMEPIAPESIEIGHHGLLVRLHAQRGLLLPQVAIEHRLTREQFLEETCRKAGLRR
                     EAWRDPESRLFGFTCEVFSENSPHEGV"
     gene            complement(5045..5713)
                     /gene="pgsA"
                     /locus_tag="VGR03_11875"
     CDS             complement(5045..5713)
                     /gene="pgsA"
                     /locus_tag="VGR03_11875"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV1995020.1"
                     /translation="MAEATGRHPRRHALMNLPNSLTLLRIFFVPVLIVILLTRSPNFE
                     FWGFSMHFEVWGVLILLLAAATDWADGYLARRRLQVTTLGILLDPIADKLLISAAFVS
                     LVDMHLVPAWMVVIIIGREFTVLGLRNIASAEGFTIEASTLGKTKMVLQVCAVAVLIV
                     GARHPSLKPLARILLWLVVISALVSAAQYFRRFWSHVDDSIKQRRRLRMLEARKKSED
                     AVPL"
BASE COUNT         1307 a         1678 c         1789 g         1222 t
ORIGIN      
        1 gggatgcgct cgatcagccg caccatgtca taactgaaat agccgatggc tcccgcgacc
       61 agcggcggca atccgggaat ccgcacggga cgaaaacgct cgacgcgttc gcgcaggaac
      121 gagatgggat cgcgctcctc ccagacgatg cggtcgcggc tctccaagac gcacgcgccg
      181 ctggcatagc gaaagatttc ctcggggtgc gcgcctgcga aggtgtagcg cgcgattttt
      241 tcgccgcctt cgacgctttc gaggagaaag gagtaccgcg cgccgtgggc gatgcgcaga
      301 taggcgctga cgggggtcag caaatccgcg gaaaagacat cgtagaccgg gatgaggttg
      361 ccctgtttcg cgaggcgctg aaattcgtgg aaatccggct ggatcatggg agtgagttgt
      421 cagtcgacag ttttcagttc acagtgaaaa gagacctatt attacattac ttgaggctag
      481 cccgaaccac tcaccagcga ccgacggcat ttggaaggag caggcggtcc tcaccgggaa
      541 gaacggtgac gaggctaggc aggccgtagg tggtcccggc ggaccggcgg aatgctagta
      601 agaaaatgcg gtctagttaa ccataacaga cccaccccct atgttttttg taagtgttgc
      661 atctaaagga cttagagtct atgtaagtga tttagaatca acacttgcgg gcattcctat
      721 aagtgttgat tctaaatggg ttagtacagc cgtgaagctg tcatccggag aggcgaaagg
      781 acgccagggc ggttcttcga gcactgggta ggcgcggaag tcgccaaaga gaagcattca
      841 gtttgtaacc ggagtttcgc ttcggaaggg cgaactactt cacagagatt atgtaccaga
      901 ttagtgaccg ctgtgtcaaa ttgaaaatca aggtagtggt cattttccgg taagcgcgtt
      961 tatctggcgc tgaggccaat ttctgttctg actgaatcag gctcgcatct gccgggacgc
     1021 tttaagctgc tgatgagctc cagaatcgaa ggtgagcatc ggcgagtagc cgactgatcc
     1081 catctcggtc cacctcatct ggaagcgtcg aagttgcccg agcagctagc gcttctgatt
     1141 ccagttgcgc gccgagctgt cgaatttcgg taagagaata cttgccccta cggatttcga
     1201 tcaactcgtc ccggtttggt ctcggcagcg tgatgcgtcc gtgctccatc aactctttcg
     1261 cttctccgta cagacggatt acatgcatgg cgtacttcgt gtcgtatcca tgtttatctt
     1321 cgagttcagc gcggtgaacg ttcttctgcc ccttctgccc gagcaatcgt ttcatctggt
     1381 cgtctgcgaa tccgaggaat ggcttcacat ggcccttcgc aaggaaaaat tcaggccggg
     1441 cggaaaagtg gtcccacgtc tcggtcgtga actcaagtgg cgcgaacaag aaatgcaaag
     1501 ccgatggatt gcccttggcg gctagtcctg cccacttcat cagggtatat aggcagacgt
     1561 ccacatctag tggcccgttg ccgccgagtt tgcctccagt cgtgaaaaca aaatgctcct
     1621 cacgttggag cccaagaact ttggtcggtg gcggaatgta aagtccgtac cagtcggtat
     1681 cgtccgtcgc gcccagtttt gcaccgtgcg cctgcgagcc gccaatacac gccatgatta
     1741 ggttttcttg atgaacgaat ccgtattggg agaagtcggg tttcactttg cggtccttga
     1801 ttctgtcaaa tggtaccgag cttccgcggc tagcgagggc gcgacttctc tccggcgcgc
     1861 agggcaccaa gcacggcgcg gcgcatggat gcttcggggg cgcgcttttg cgtccagatt
     1921 tcccattgag cgaatccctg ggcgagaaac atctcgacgc cggagacggt agcgattccc
     1981 ttttgcgcgg cgagcttcaa gagttgagtc ctctgcggac ggtagatcaa gtccatcacg
     2041 atccgacagt gtaactcgcc gggcgcgaga ggcgaaattc caccgtgcgg gtgcatgccg
     2101 accggcgtgg cgttgaggat ggcgtcaaag aattccgtgc gtaaagcgcg gcgcggcact
     2161 acttctccgc ccacggcccg ggccagttct ttcgctgctt tctcacggcg cgcacaaatt
     2221 ccgacgatgg cgcccgcgcg cgacagcgcg aatgcagccg cacgtgccga tccgcccgcg
     2281 ccgaagatca aggcgcggct ccttttgatt tgcagtttct tttccagtgc gcggagcacg
     2341 ccgacgtaat ccgtgttgca gccgtaaagc gacccgtcgc tccggacaac aacggtattc
     2401 acggcaccga tgtctgccgc cagcggctcg cattccttca ggtgtttgag gatggcctgc
     2461 ttgtgcggga tggtgacgct gaaaccgcgg acaccgaact ctggaacagc cgcaagaaaa
     2521 tcgccgagct gatgcaccag aaatgggaga tagacggcat caacgcggcg cgccgcgaat
     2581 cccgtgttgt gtagcaacgg agaaagagaa tggctgacgg gatcgccaat cactccatag
     2641 acccgcgtgc ggcgcgtgag agcgtgggcg cggtaaagat gtttcatttg gtgcagtgaa
     2701 acctggccgg gggcagtcgc cgcggcaaca ggggcgtaag ccagcgcact gccctcgcgc
     2761 aaggcgagga ttcgcgccgg caggccagct tctcccattg gaacggcgac aaaattttcc
     2821 gaacttcgcg ccaggcgcaa gagccgaacg ctgtcctgga tgctccgagc ttcggccgcg
     2881 atcttcacgg catcgacccc gccgtcggct ggcgggttca tgcttcgagg caaatccggc
     2941 gtgcgctcaa agtcgtgtac ggaaagcatg acacgcggcg gcacgggata ttcccggacg
     3001 gactggccag gaagcttgcg caaggtttcc acttcgagat cgcaccattg gcagccggct
     3061 tcgcgggctt gaatcagcca gtagagttcg cggtcaacgc ctccggcaaa tttgcctccg
     3121 ccttcgcggc ggcggcaggt ggcgagaaat gtggcattcc gagatttgtc ctttcccagc
     3181 cagccgagga atcgcgcacg ctccgcgtca ctgctcagcc aatcaaggcg caactcgacg
     3241 gtcctggttt gccttaaggc cacgcgcacc agctttgaca tttcggccgc cgtggaggcc
     3301 gccacgaccg cgcaaatccg ccgtaacccg gtgccaaaga ggcccatacc ctgccttgtg
     3361 cccccaatct attgaaaaca cagctagtag tatgccgtat caaaagtgta aagcaacctc
     3421 ggccatttgg ttgacgcgtg gggtaggccc tgatactttc gctaggaata gtccatgcac
     3481 ggcccgtagg gcgtgttttc gaaggagtta tggaatgcgc gctagaatag ctgttttctc
     3541 tctatcggtc ttcctgggta ctgcggcaag ttcggcacaa aacgcgcccc aggctgctcc
     3601 ggcaattcct caagtcgact acagtacggt gaattgttca ggattcgtta cggaccagaa
     3661 ggtgccggac gagatccggc tcgtctctgc ggagcaatcg aactacaaga tcacctttgc
     3721 gcgcggcgac tacgtgcaca tcaatcgcgg ccaggacaaa ggtgtgcgcg tcggcgaccg
     3781 gttctcggtt gtgcggccgg acaaggatcc cacagacgtg ccatggttca agtggcagac
     3841 gaagctcctg aaagccatgg gcacgcccta tatggacttg ggacagatcc gggtggtcag
     3901 cgtccagccc aaggtttcca tcgcacaggt caacttctcg tgtgcgttta tgcagcgagg
     3961 agatatctta caaccctacc aagatcgtcc tgcaccgccg ttcaaagatg ctgtcgcctt
     4021 cgatcacttt gcgcctgtga gcggcaaacc tgtggccatg atggtggctg gaaaagacta
     4081 cggacaggtc tacggtaaac tcagcgcggt ttacgtaaac ctcgggagca accaaggtgt
     4141 caaggtcggc gattacttcc gaatcttccg gtatcaaggc tcaatggcag agaccgtgcc
     4201 acaaaacaag ggttatcagt actcgatgta cggctttggg agtgcccccg tgcgctacga
     4261 atggaatgac ctgccgcgcg aagtgctagg cgagggcatt gtcatcagcg tcagccgcaa
     4321 ttcatcgacg atgataatca ctttcaattc gatcgaagtc tacgcgggcg actacatcga
     4381 aatcgagtag gagtccggcg ccccgctgaa tggttcaggc ccgctgcggt ttctccgcgc
     4441 gggccttttg ttttgtttgg ccgttcgatt tcgagcttca caacgtagat gaagcgttca
     4501 gactccctcg tgaggtgaat tctcagagaa gacctcacat gtaaatccaa agaggcgaga
     4561 ctcgggatcg cgccaggctt cgcgacgcaa ccccgccttg cggcaggttt cctcgaggaa
     4621 ctgctcgcgc gtcagccgat gctcgatggc aacctgggga agaagaagcc cgcgttgcgc
     4681 gtgtagacgg acgagcagcc catgatgccc gatttcgatg gattcagggg cgatcggttc
     4741 catgggtgaa agcaatgaaa tttcaatact aagttcgcca agctggtccg tcctcatagg
     4801 ggcgaaccgt ggatcttcca gcgctgcgct ggcagcgcaa cgaacaatgg cttctccgag
     4861 cggctcgttc gcttcaatga cgccgataca accctgcagg cgattgcgca cgtgaagggt
     4921 cacgaagacg ctacggcgtt ccgcaaagat tccttcgcga gggaatacat caggaagctt
     4981 gtggtgggag acggcttcaa caacagccgt ccgggctagt tgcagagcgg cgccccggtc
     5041 cgcttcagag aggaacggca tcctcggact tcttgcgggc ttcgagcatc cggagccggc
     5101 ggcgttgctt gatgctgtcg tcgacgtggc tccagaagcg ccggaaatac tgggcggcgg
     5161 agacgagcgc gctgatgaca acgagccaaa gcaagattcg cgccagcggc ttgagcgacg
     5221 ggtgccgtgc cccgacgatg agtacggcca cggcgcagac ctgcaaaacc atcttcgtct
     5281 ttccaagggt ggatgcttcg atcgtgaatc cctcggccga agcgatattc cgcagaccga
     5341 gaaccgtaaa ttccctgcca atgatgatca ccaccatcca cgcgggaaca aggtgcatgt
     5401 ccaccagcga aacgaacgcc gcggagatca agagcttgtc ggcaatgggg tcgagaagaa
     5461 tgcccagagt cgtcacctgc aggcgccgcc gagcgaggta tccatccgcc caatcggtcg
     5521 ctgccgccag gagcaggatc aacacacccc agacctcgaa gtgcatggaa aatccccaga
     5581 actcgaagtt gggactgcgg gtgaggagga tcacgatgag gacgggaacg aaaaagatcc
     5641 gcaacagcgt cagtgagtta ggaagattca tgagcgcatg acggcggggg tgccgccccg
     5701 tggcttcagc caccgcagtt tcactataga cttggtgtaa ggatgcgtca acacgccaga
     5761 ggcggctccg ggagcgcccc aaggagtctc aatccattgg ctttctggtc ttgggtagtt
     5821 acttcgggat tacctcagcc ccaagagtac aagcaaagaa agctgtactc tttcgcaacg
     5881 cgactgctgc aaatctgtgc aaatgtgcga agtgcccact ggtgacgagg cgggcgagac
     5941 gggacgtata gatgttcctg agaaccgcat aactctttta ttttcaatgc gctgca
//
LOCUS       DASYVO010000350         5258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_110986, whole genome shotgun sequence.
ACCESSION   DASYVO010000350 DASYVO010000000
VERSION     DASYVO010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110986"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VGR03_11880"
     CDS             complement(<1..480)
                     /locus_tag="VGR03_11880"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEV1995021.1"
                     /translation="MVHAVADQPQCWPALPLSSWKDTYATLHMWTQIVGKVRLRLTPV
                     VNHWWNVPLYVTARGLTTSRMAYGTRAFEVWFDFIRHQLVLETSDGLVKTLSLEPRSV
                     AEFYQEFMELLRSAEIELKIWRMPVEIPDPIPFDQDRVHASYDRASVERFWRVLLSVD
                     "
     gene            complement(593..958)
                     /locus_tag="VGR03_11885"
     CDS             complement(593..958)
                     /locus_tag="VGR03_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1995022.1"
                     /translation="MSPTLANGKICYIEMPATDIADSADFYKQVFGWSIRKRGDGSTA
                     FDDAVGEVSGTWVLGRPPAAKPGLLFYIMVDSVAATIDAVVAHGGEIVQPIGADAPEI
                     TARFRDPGGNVIGLYQNPA"
     gene            complement(1013..1333)
                     /locus_tag="VGR03_11890"
     CDS             complement(1013..1333)
                     /locus_tag="VGR03_11890"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1995023.1"
                     /translation="MPFVDTRNLKVIERLPGWYGRYFHSANMTFAHYDFTRGSSIHEH
                     FHPQEEVYEVIEGELEVTIDGEAHVARAGLVAIVPGNVRHSVKALTDGRAIIVDYPLR
                     RDFD"
     gene            complement(1475..2410)
                     /locus_tag="VGR03_11895"
     CDS             complement(1475..2410)
                     /locus_tag="VGR03_11895"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEV1995024.1"
                     /translation="MKPALIVHGGAWDIPDEAVDACKSGCHRALTAGWAILSRGGSAL
                     DAIEAAIIVLEDDPVFDAGYGSHLNLDGHVECDAIVMNGATLRAGAAATLQRVKNPIQ
                     VARKILENCPHMMLVAEGAERFAKDQGIQLCKPEEMVSEAEWEAWQKCKGDKHAAEHH
                     RGHEQGTVGAVALDANGGLFAATSTGGTCCKLPGRVGDSPLIGCGCYADSEAGGVSCT
                     GYGEAIMKVVLAKSAVDLLRRPATCVDTPTNISCGTSTADLAAREAVHLLAKRTHATG
                     GLILLDRRGNPGFAFNTPRMAYAFVAPDGSFLTAV"
     gene            complement(2563..2883)
                     /locus_tag="VGR03_11900"
     CDS             complement(2563..2883)
                     /locus_tag="VGR03_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995025.1"
                     /translation="MNWGTGPALGLLTVSGYLLFMAGVALVWRSRSDFSVWFQDELSV
                     FRRNFSRYTPAGPFYAIREESRFKAIPVSFVRSITRFPRSRINGGPILLFIGFLLLIL
                     DFFV"
     gene            complement(2943..3422)
                     /gene="greA"
                     /locus_tag="VGR03_11905"
     CDS             complement(2943..3422)
                     /gene="greA"
                     /locus_tag="VGR03_11905"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV1995026.1"
                     /translation="MSDPLAKIKKKLQDEIAQLSHELNVELPKEIAVARAHGDLSENA
                     EYKYAKERQGYVNLKITHLKKRMGDLSMLNLTNIPKDRSGYGSRLTVLDLQRDAKIEY
                     TLVSTEEADVEKGLISTTSPIGKALLNRKVGDEIEVVTPAGKKEFELVRLVTIHDGE"
     gene            complement(3575..4030)
                     /locus_tag="VGR03_11910"
     CDS             complement(3575..4030)
                     /locus_tag="VGR03_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995027.1"
                     /translation="MLAQPDLQKKSAFKNPFLYSWAALVIAALVVSWILAARWLENRG
                     IEKRAKEERTQKQQEQDRIALEQLGGKELAIQNFYAIPGAVRRGETVQLCYGVANAKT
                     VKLEPQPNPVWPSYSRCVDVTPTKSTTYTLTIADAAGNTRTQTLEVKVR"
     gene            complement(4100..5080)
                     /gene="maeA"
                     /locus_tag="VGR03_11915"
     CDS             complement(4100..5080)
                     /gene="maeA"
                     /locus_tag="VGR03_11915"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate-decarboxylating malate
                     dehydrogenase"
                     /protein_id="HEV1995028.1"
                     /translation="MCTRSILDRYRDRLLTFNDDIQGTAAVALGAILAAVRVAGSSFR
                     DQHIVFLGAGAASTGVADYLRSALVAKGLSEAEARGRVWMVNRGGLLHDGRVDLTPEQ
                     RIYAQPLSRVADWPRGSDDKIGLSDVIKHVRATVLIGLSTLPLAFTEPIVREMASKIE
                     RPIILPLSNPTDRSEARPEDLLRWTEGRALVATGSPYAPVRFGDRTIPISQCNNVYIF
                     PAVGLAVVASRPFADSAGTRRITDGMMLAAARALAEHSPALRDPSAPLLPTLKNIRNV
                     AVEIACAVAREAQRSGLAPRADSSSSDSLRSRILASQWFPAYPTYLPAFG"
     gene            complement(5046..>5258)
                     /locus_tag="VGR03_11920"
     CDS             complement(5046..>5258)
                     /locus_tag="VGR03_11920"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF012608.4"
                     /note="malic enzyme; oxaloacetate-decarboxylating;
                     NAD-dependent; catalyzes the formation of pyruvate form
                     malate; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEV1995029.1"
                     /translation="LPIVLDVGTNNEERLRDPEYLGWRHARITGTDYDDFVDAFVKAV
                     KEVLPATILQWEDFAGVHALHPRPLP"
BASE COUNT         1110 a         1381 c         1732 g         1035 t
ORIGIN      
        1 atccacagat aacaggacgc gccagaatct ctcgactgat gcgcggtcgt acgaggcgtg
       61 cacccgatcc tgatcaaaag gaatcggatc gggaatttcg accggcattc tccagatctt
      121 gagttcaatt tctgccgatc gaagcaattc catgaattcc tggtagaact cggccaccga
      181 gcgcggctcc aacgagagtg tcttcaccag tccgtcactc gtctccagca caagctgatg
      241 tcgtatgaag tcaaaccaga cttcgaatgc tcttgtgccg tatgccatcc gtgatgtcgt
      301 cagcccgcga gccgttacgt acaacgggac attccaccag tggttcacga ccggcgtcag
      361 acgcagccgg accttaccaa cgatctgggt ccacatatgc agcgttgcat acgtgtcttt
      421 ccacgaactc agaggcagag cgggccagca ttgcggctga tcggcgacgg cgtgaaccat
      481 agtgaagttc ctacgcgaac ctgtgttgcg ggaatggaca tcgccgggcg atgtcgtcct
      541 tttcagcttt cctgcctcag acaaccagag gcagacctgt cctatccgag cgtcaggcgg
      601 gattctggta gaggccgatc acatttcctc ccggatcgcg gaacctcgca gtgatctcgg
      661 gcgcgtccgc gccgatcggc tgcacgatct cgcccccatg ggccaccacc gcgtcaatgg
      721 tcgccgccac gctgtccacc atgatgtaaa agaggagacc gggctttgca gcgggcgggc
      781 gtccgagcac ccaagtaccg ctcacttcgc cgactgcatc gtcaaacgca gtgctgccat
      841 cgccgcgttt ccggatgctc cagccgaaga cctgcttgta gaagtcggcc gagtccgcaa
      901 tgtcggtggc gggcatctcg atgtaacaga tcttcccatt cgcaagggtg ggagacatga
      961 ggattccttt cagggcgtcg gcattctgga gtcagttgcc gccatgctgg cttcagtcga
     1021 aatcgcgcct aagcggatag tctacgataa tggccctgcc atcagtgagc gcctttaccg
     1081 aatggcggac attgccaggt acaatcgcga ccaagcccgc tcgggcaacg tgtgcctcgc
     1141 cgtcaatggt cacttcgagc tcgccttcga ttacctcgta gacttcttcc tgaggatgga
     1201 agtgctcgtg gatggacgag ccacgcgtga agtcgtaatg cgcgaatgtc atgttcgctg
     1261 agtggaaata gcgcccatac caacctggca agcgctcgat gactttgagg ttgcgggtgt
     1321 caacaaatgg catagagctg ctcctatggc gcatcgtagc acggcaggtc cgcgtggacc
     1381 cgaatagatc tttctcctgc aagtaattgg tttccggtga gctacttgcg ggcttgcccg
     1441 gacgccagac acgagagtac ggcaaagagc ggccctagac ggcggtgagg aaggagccgt
     1501 cgggggcgac gaaggcgtag gccatgcggg gggtgttgaa ggcgaagccg ggattgccgc
     1561 ggcggtcgag gaggatgagg ccgccggtgg cgtgagtgcg tttggcgagg agatggacgg
     1621 cttcgcgggc ggcgagatcg gcggtggagg ttccgcaaga aatgttggtg ggcgtatcca
     1681 cacaagtggc ggggcggcga aggagatcga cggcagattt ggccaggacg actttcatga
     1741 tcgcttcgcc gtagccggtg caggagacgc cgccggcttc ggagtcggcg tagcagccgc
     1801 agccgatgag aggggaatcg ccgacgcggc cgggaagttt gcaacaggtg ccgccggtgg
     1861 aagtggcggc gaagagaccg ccattcgcgt cgagggcgac ggcgccgacg gttccctgct
     1921 cgtggccacg gtgatgttcg gcggcgtgtt tgtctccctt gcacttctgc caggcttccc
     1981 actcggcttc gctgaccatt tcctccggct tgcagagttg gattccctgg tctttggcga
     2041 agcgctccgc gccttcggcg acgagcatca tgtgcgggca attttcgagg attttgcggg
     2101 cgacctgaat gggattcttg acgcgctgca acgtggcggc agcgccggcg cgcagcgttg
     2161 cgccgttcat gacgatggcg tcgcattcga cgtggccgtc aagattgagg tgcgagccgt
     2221 agccggcgtc gaagacgggg tcgtcttcga ggacgatgat ggcggcttcg atggcgtcca
     2281 gagcggagcc gccgcgggag aggatggccc acccggcggt gagagcgcga tggcagccgg
     2341 atttgcaggc atcgacggct tcgtcgggaa tgtcccacgc gccgccgtga acgatgagag
     2401 caggtttcat cgagatgagg atggcaaagg gagggtggtt tgtctaggag agtcggaccg
     2461 aggtaacgaa agaagcaaag aagtaatgaa gtaaagaaac gaagaaaaaa gatttaacgc
     2521 agaggacaca gagagcgcag agtccgcaga gaagaaaaaa cttcagacaa agaaatcgag
     2581 aataagaaga aggaaaccga tgaagagaag tatggggccg ccgttgatgc ggctgcgcgg
     2641 gaagcgcgtg atcgaccgca cgaaagaaac cggaatggct ttgaagcggg attcttcgcg
     2701 gatggcatag aacgggccgg ccggggtata gcgggagaaa ttgcggcgga aaacggaaag
     2761 ctcatcctgg aaccacacgg aaaaatcgct gcggctgcgc cacacgagtg cgacaccggc
     2821 catgaataag agatagccgg aaacggttag tagcccgagc gccgggcctg ttccccaatt
     2881 cataagtcct cacaggcggc ccatacgatt tgcggaatag gacgcccgga acaacaataa
     2941 gtttactcgc cgtcgtggat ggtgacgagg cggacgagtt caaattcttt cttgccggcg
     3001 ggtgtgacca cctcgatttc gtcgccgacc ttgcggttga ggagggcttt gccgatgggg
     3061 gacgtggtgg agatgagtcc cttctcgacg tcggcttcct cggtgctcac gagagtgtat
     3121 tcgatttttg cgtcgcgctg caggtcgagg acggtgagcc gcgacccgta tccggaacgg
     3181 tccttcggga tattggtgag attcagcatg ctgagatcac ccatgcgctt cttgagatgg
     3241 gtaatcttga gattgacgta gccctggcgc tctttggcgt acttgtactc agcgttttcg
     3301 gagaggtcgc cgtgagcgcg ggcgacagca atctccttgg gaagctcgac gttgagttcg
     3361 tgcgagagct gagcgatttc gtcctggagc tttttcttaa tcttggctag tgggtcggac
     3421 atggtagtcg gttgtgcgtt cgccaaaaaa ttcggcggag tctgggagca acggatggtc
     3481 tcccggaccc cgcgtaagga gacattatag atgcggggga attgaagtca caaggggaag
     3541 cgcaagaatc ggcgcgaaga ggcattaccg gcattcagcg aacctttact tccagggttt
     3601 gggtgcgggt gttgccggcg gcgtcggcga tggtcagcgt gtaggttgtt gatttagtgg
     3661 gagttacatc aacgcaacga gaataagagg gccagacggg attgggctga ggttcgagct
     3721 taacggtctt ggcgttggcc accccgtaac acaactggac ggtttcgccg cggcgaaccg
     3781 ctcctggaat ggcgtagaag ttttggatgg cgagctcctt tccgccgagt tgttcgagtg
     3841 cgatgcgatc ctgctcctgt tgtttctggg ttctctcttc tttagcgcgc ttttcaatgc
     3901 cgcggttttc cagccagcgt gcggcaagaa tccagctgac gacgagcgcg gcgatgacga
     3961 gtgcggccca ggaatagaga aagggattct tgaaggcaga ttttttttga agatccggtt
     4021 gagccagcat agatgcaccc cctagtggta agagcttgct tggaagtatt cggacaacgg
     4081 ccaatgcgga tctctctaac tatccgaatg ccggaaggta agtagggtaa gccggaaacc
     4141 attgagaggc gagaatacgt gaacggagcg agtcggaaga cgaagaatca gcgcgagggg
     4201 cgagcccaga gcgctgcgct tcccgggcca cggcacacgc aatttcgacg gcgacgttac
     4261 ggatgttttt cagcgtgggc aaaaggggag ccgaggggtc tctcagcgcc ggagaatgct
     4321 cggcgagtgc gcgggcggca gcgagcatca tgccgtcggt gatgcgccgg gtcccggcgg
     4381 agtccgcgaa ggggcgcgat gcgaccacgg cgagccccac ggctggaaag atgtacacat
     4441 tgttgcattg cgaaattggg atggttcggt ctccgaatct aacgggtgcg taaggtgagc
     4501 ccgtcgcgac gagggcgcgg ccctccgtcc aacgaagtaa gtcttccgga cgggcttcgg
     4561 agcggtcggt tggattggaa agcggcaaaa tgatgggccg ttcgatttta ctggccatct
     4621 cgcgcacgat cggctcagta aaagcaagag gtagtgtgga caaaccgatg agtacagttg
     4681 cccggacatg cttgatcacg tcggaaagtc cgatcttgtc gtcactgccg cgaggccaat
     4741 cggccacgcg gctcaaaggc tgggcgtaga tgcgctgttc aggcgtcaga tctacccgtc
     4801 catcatgaag aagaccgccc ctattcacca tccacacgcg accgcgggct tctgcttctg
     4861 aaagtccttt cgcaacgagt gccgagcgca ggtaatcggc gacgcctgta ctggcggcgc
     4921 cagccccgag aaaaacaatg tgctgatctc ggaaggagga accggcaacg cgcacggcgg
     4981 cgaggatcgc accgagcgcg acggcagccg tgccctgaat gtcgtcgttg aaggtgagca
     5041 atcgatcacg gtagcggtcg aggatggagc gcgtgcacac cggcgaagtc ctcccactgc
     5101 aggatggtgg cgggaagaac ttccttgacg gctttaacga aggcgtcgac aaagtcatcg
     5161 tagtccgtcc cggtgattcg ggcatgccgc cagcccaggt attcggggtc gcgaaggcgc
     5221 tcttcattgt tggtgccgac atctaggacg atgggaag
//
LOCUS       DASYVO010000351         7491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_232994, whole genome shotgun sequence.
ACCESSION   DASYVO010000351 DASYVO010000000
VERSION     DASYVO010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232994"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     repeat_region   160..854
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=226..261
                     /rpt_unit_seq="gctagggtttgccgggaatcgctcctgagatacact"
     gene            complement(898..4176)
                     /gene="cas9"
                     /locus_tag="VGR03_11925"
     CDS             complement(898..4176)
                     /gene="cas9"
                     /locus_tag="VGR03_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEV1995030.1"
                     /translation="MAEEAPPPLILGLDLGATSVGWALIGSANGVPAGIVRAGSRIFD
                     ADRATDIKAGRDKPRGQERREARLQRRQIWRRARRAREIFWMLQGWGILPPGASSQPE
                     QRQELLNQLDRQILGSAWFKQRSSAQDIPEPQQVMPYLLRASALDEPLEPFFLGRALY
                     HLAQRRGFLSNRKEVAPGKKEKTEEEGEVKKSIGELREKMTESGARTLGEYFSRQRPS
                     GEKRIRQRWTARAMYEEEFEKIWAAQADFRQETFTAARKKLLWRAIFFQRKIKFPENL
                     VGKCELEPGKRRAPKYLLPSQRFRLLQGVNNLRVSPPGEVERPLASGERDQLVEALEV
                     RGDMKFHEIRKLLGLHKSCRFNLESEGEDRLLGNRTAAKLGEALGERWQAMTPAERNG
                     IVEYLWSFEKADKLAGAAAKKWGLDPETAEKLAQVAFEADYMNVSTAAMRRLLPLLEA
                     GRSFAEARKELYPEKSRPQQPLDWLPPVQKSRRRPGWEERSSARHAWGRDVRNPIVVR
                     CLSEVRKVVNAIIRTYGKPTEIRIELARDLRNTRRQRKNISKRNWDNRSLREQAEQKI
                     TDEIPGFKPSRRDILKVRLAEECHWCCPYTGKSISMTALVGPESQFDIEHILPYAKFL
                     DDSFANVTLCEAEHNRNVKQRRAPSEAYGGNPELYEQIKGRVAKFSGPMAKEKLRRFR
                     LTPAEIEEELKEFSSHLLNDTRYATRLAADYLGLLYGGGVDEARTRRIHATSGRVTAI
                     LRGEWGLNSILADGATEDGGERPKYRGDHRHHAVDAIVTALTDDGTIQMLNRAAERAP
                     SERRRRFASIESPWNNFGDSVRREIEKMVVSHRVSKRVRGPLHEETIYSWPIPIAEKA
                     KAKRGKPGVTQEYRVHKPLAQMSAQEAKDIADPAVRELVQKKLEEVGGEPKKVFSNEA
                     NLPFFSKEGRRVLVRGARVRKRVPVSPLGEGFGARYVTRESNHHVEIFADLDRDGREI
                     EWNASVVSLMEAYRRKRAHEPVVRTSHGPQTEFQFSLAPGEVIECEDEEKGSRRLLLV
                     RGCTQLSAGAVQIFLAPIEDARLKKEQVDDGAYLRPASKTLRRWKARKVRVTPLGDVM
                     DAHD"
     gene            5101..6537
                     /gene="cysS"
                     /locus_tag="VGR03_11930"
     CDS             5101..6537
                     /gene="cysS"
                     /locus_tag="VGR03_11930"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV1995031.1"
                     /translation="MAEIQLHNTFTGKKEPFTTLHPGEVRMYACGPTVYDYAHIGNFR
                     TFVFQDVLRRFLELRGYRMNQVMNLTDVDDRIIANAAAAGASIRDYTEKYAQAFFADC
                     RALNFEPPEHWIRATEHIEDMVVLIERLRAKSLTYESEGSIYFRIAKFPAYGRLSKID
                     VSGIQAGARVDVDRYEKESARDFALWKAPKPEEQFWETRIGRGRPGWHIECSAMAMLY
                     LGETLDIHSGGIDLVFPHHENEIAQSEAATGKQFVRYWLHAEHLLVEGEKMSKSLGNF
                     YTLRDLFAKGHKPSTLRFLLASVPYRKQFNFTSEGLQQAASSVQRVRNLAARLKEEKF
                     PGGKETALSARARNAEEEFEAALADDLNTAQALAAVFDLVRDANIAMDKGEFQKGDAA
                     PVQAFLTKFDRVFAVLRDDDGEKLRALGFAGAAELTDAEVEKQVAQRNEARKRRDFAA
                     GDRIRKELADRGIILEDTKDGGVRWKRK"
     gene            6744..>7491
                     /locus_tag="VGR03_11935"
     CDS             6744..>7491
                     /locus_tag="VGR03_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV1995032.1"
                     /translation="MAVTVESKLPRLITALPGPHAKRVIEYDARFVSPSYTRDYPFVA
                     KRGRGATIEDVDGNTFLDFAAGIAVCSTGHCHPRVVEAIQRQAAELIHMSGTDFYYES
                     MPQLAERLARTVPGSEGKRVYFGNSGTEAVEAALKLARYATRRDKVIAFYGAFHGRTM
                     GALSLTASKSVQRKGFGALLSGIEHIPYPYAYRCALGHTAETCGAEILEMLEREMFKR
                     LFDPEEIAAIIIEPIQGEGGYVPAPAFFLQE"
BASE COUNT         1431 a         2395 c         2093 g         1572 t
ORIGIN      
        1 aaacccgccg ccgtctggcg ggcgcggtgt ctctttgtaa ttccgggcaa gcgccatcgg
       61 ccggaggccc gccgtagtct ggcgggcgcg ccagaagtta gcccagcccg taagggctgg
      121 gaaagaaagg tagcaaggag aatcgccagc gccgggccgt ctagggtttg ccgggaatcg
      181 ctcctgagat acactattac cggccctcat caccgcctac tgcgagctag ggtttgccgg
      241 gaatcgctcc tgagatacac tgcaggccat cctcgagagc agcgaccgca agctagggtt
      301 tgccgggaat cgctcctgag atacactaac ccccggatct tgtgcctccc ccgctgcgct
      361 agggtttgcc gggaatcgct cctgagatac actaactagt cgcaagggac cgcaagatcg
      421 cgcgctaggg tttgccggga atcgctcctg agatacactg aaaacttccg gttctgcacg
      481 ttccgtcctg ctagggtttg ccgggaatcg ctcctgagat acacttgagc gaggcgctaa
      541 gtcgccgcga aaggggctag ggtttgccgg gaatcgctcc tgagatacac tttccacgct
      601 tctccgctcg cttgtccacg cgctagggtt tgccgggaat cgctcctgag atacactcct
      661 ccagccgctc cctcgctatc cgcttgggct agggtttgcc gggaatcgct cctgagatac
      721 actgcaagga ggccagccat gacccgcaag gcgctagggt ttgccgggaa tcgctcctga
      781 gatacactgt aaatagcgag cgtggagggc aacacagagc tagggtttgc ctggaatcat
      841 cgctcctgag atacactccc cgccgcgctg gctgcgctct gaaactccgc cagccggtca
      901 gtcatgggca tccatcacgt ctccgagcgg cgtcacccgc acctttcgcg ctttccaccg
      961 gcgcaacgtc ttcgaagcgg ggcgaagata ggcgccgtca tctacctgct ccttttttag
     1021 cctggcatct tcgattggcg ccaaaaaaat ctgcactgct cctgccgaca actgcgtaca
     1081 gccgcgcacc agcaggagcc ttcggctccc tttctcctca tcctcgcact cgatgacttc
     1141 gccgggcgct aaagaaaact ggaactccgt ctgcggacca tgactcgtcc gtacaaccgg
     1201 ctcgtgcgcg cgtttccggc gatacgcctc catgagcgaa acgaccgaag cattccactc
     1261 gatctcccgc ccgtcccggt ccaagtcggc gaatatctcc acatggtggt tggattcacg
     1321 ggtcacgtag cgcgccccaa atccctcccc gagtggtgaa actggcaccc gcttccggac
     1381 gcgcgcaccg cgcacgagaa cgcggcggcc ctccttcgag aaaaacggca gattcgcttc
     1441 gttcgagaag actttcttcg gctcgccgcc aacttcttcg agtttcttct gcaccagctc
     1501 tcgcacggcg ggatcggcaa tgtccttggc ctcctgggcg ctcatttgag cgagcggctt
     1561 atgcacccgg tattcctgcg tcacgcccgg cttcccccgc ttcgcttttg ctttctcggc
     1621 tattggaatc ggccaggaat aaatggtctc ttcgtgcagc ggcccgcgca ctcttttcga
     1681 cacccggtgg gaaacaacca tcttctctat ctcccgccgc accgagtcgc cgaaattatt
     1741 ccacggggat tcgatggacg cgaagcgccg ccgccgctcg gacggcgccc gctcggctgc
     1801 ccggttcagc atctgaatcg tcccgtcgtc cgtcaaagcg gtcacgatcg catccaccgc
     1861 gtgatgccgg tgatccccgc gatatttcgg tctttcccca ccgtcctcgg tcgcaccatc
     1921 agcgaggatc gagttcaatc cccattcgcc tctcaggatg gccgtaacgc ggcctgatgt
     1981 ggcatgaata cggcgggtgc gcgcctcgtc cactcctccc ccgtagagaa gcccgagata
     2041 gtcggcggca agcctcgtcg cgtatcgcgt gtcgttcagc aggtgcgacg agaattcctt
     2101 gagttcctct tcgatctccg cgggtgtcag tctgaacctg cgcagttttt ccttcgccat
     2161 cggccctgaa aacttcgcta ctcgcccttt gatttgctcg tacagctcgg gattcccgcc
     2221 gtaagcctcg ctgggtgccc gccgctgttt cacatttcgg ttgtgctcgg cttcgcagag
     2281 cgtgacattt gcgaaagaat cgtccaggaa cttcgcgtag ggaaggatat gttcgatgtc
     2341 gaattgcgat tctgggccga ccaacgccgt catcgaaatg ctcttgcccg tgtacggaca
     2401 gcaccagtgg cactcctcgg ccagcctcac ctttagaatg tcgcggcgcg agggcttgaa
     2461 gcccggaatc tcgtccgtga tcttctgctc cgcctgctcg cgcagagagc gattatccca
     2521 gttcctcttg ctgatgttct tgcgttgcct gcgcgtgttg cgcaggtccc gcgctagctc
     2581 gatccgaatc tccgtcggct tgccataggt ccgtatgata gcgttcacca ctttccggac
     2641 ctcgctcaag cagcgaacca ctattgggtt gcgaacgtcg cggccccagg cgtgcctcgc
     2701 tgacgagcgc tcctcccagc caggccgtcg ccggcttttc tgcactggcg gcagccaatc
     2761 gagaggttgc tgcgggcggg atttttcggg atagagttcc ttgcgggctt ctgcaaagga
     2821 cctgcctgct tctagcagcg gcaaaagtct tcgcattgcc gctgtcgaga cgttcatata
     2881 gtccgcctca aaagcgacct gcgccagctt ttccgccgtc tccgggtcaa gcccccattt
     2941 cttcgccgca gcgccggcca gtttgtcggc tttctcgaaa ctccacaagt actccacaat
     3001 cccattgcgt tcggccggtg tcatcgcctg ccatcgttca ccgagcgctt cgccaagctt
     3061 cgccgcagtt cgattgccca aaagcctgtc ctcgccttca ctctcgagat tgaaccggca
     3121 tgacttgtgc agcccaagca gtttgcggat ctcgtggaat ttcatgtctc cccgtacttc
     3181 cagggcttca accaattggt ctcgctcgcc ggacgcgagc ggacgctcca cttcgccagg
     3241 cgggctaacc ctcagattat tgacgccctg aagcagccgg aatcgctgcg agggtagcag
     3301 atacttcggc gcccggcgct tgcctggctc caattcgcac tttcccacga gattctcagg
     3361 aaacttgatt ttccgttgga agaaaatggc ccgccagagc agtttcttgc gcgcagcggt
     3421 gaacgtctcc tgacggaaat ccgcctgtgc cgcccagatt ttctcaaact cctcctcata
     3481 catggcccgg gctgtccacc gctgccggat gcgcttctca ccagagggcc gctgccgaga
     3541 gaagtattcg cccagcgtgc gtgcgcccga ttcagtcatc ttctcccgaa gctcgccgat
     3601 gcttttctta acttcgccct cttcctccgt cttctctttc ttgccgggag ccacttcttt
     3661 gcgattgctg aggaacccgc gcctctgcgc gagatggtat agcgcacgcc cgaggaagaa
     3721 cggttcgaga ggttcatcca gggcggaagc cctcaacaga taaggcatca cctgctgagg
     3781 ttcgggaatg tcctgcgcag acgagcgctg cttaaaccaa gctgagccaa gaatttgtcg
     3841 gtcgagctgg ttgagcaatt cctgccgctg ctctggttgg gagctagcgc ccggcggtag
     3901 gattccccag ccttggagca tccagaaaat ctcgcgggca cgccgggcgc ggcgccagat
     3961 ttgccggcgt tggagacgcg cttcgcgccg ctcctggcct cgcggtttgt cgcgccctgc
     4021 cttgatatca gtggcccggt cagcatcgaa gatgcgcgag ccggcgcgca cgatccctgc
     4081 gggtacgccg ttggcgcttc caatcagcgc ccaaccgacg gaagtcgcgc cgagatcaag
     4141 acccaggatt aaagggggtg gggcctcttc tgccatatag cgcttgactc cttataacag
     4201 tgtgatagag tggcgcctag tgtatctcag gagcgattgt cccggcttat cttagtaaga
     4261 ctttcgttcc gcagggcaac gccgttcggc gaccctaagc gccccccagt ggggcgcttt
     4321 ttcattcccc ccacgccgct ccagaaatcc cttgactgcg ggttattctc atagtaaact
     4381 agttcatgaa taatcggcca agccctctcg gctcccgcaa ctgctttaga atccgcactt
     4441 ccgataacct gctcccgcaa gccctgccga atcaacactt acagaccccc ctcggatctg
     4501 cggaaaccaa aggacttata gcgcgagaat ttctcccgca actcctttat tttcaacact
     4561 tacgagcatc tcttgtaagt gcggaaaaca aaagacttat aacccctttg gattcagcac
     4621 ttacgagaaa gcactctcgt aaccccttta gaatccgcac ttccgaaaaa cacgggggag
     4681 ggggctctta ccctgtggaa atctctcggt ccgggtttca cccaacgccc gctagtcacc
     4741 aatcatcgat aaccaatccc cagtcaccag tcacgaatca ccagtcacta accaccattc
     4801 atctcttttc ttttcatgct gttgtgcact cttttcactc ttgccgtgcg caggaatcgc
     4861 cgcaacccct ttctttccat gcgcttccgc actcttgcga aaacaatagg gggtgggggg
     4921 gtgaagatgt cctctaacca tccggcaagc aacgcccgtc gtcatcgtcc agccatccgc
     4981 ctcctgtctc tagcatccag cctctacaac acacgggtca cgcaccacgg ttcacggatc
     5041 gcttccttgt cgggtacaat ctctcgtttg ctcccagtcg gaaagcgatc tgcgcgggct
     5101 atggccgaga tccagctcca caacacattc acgggaaaga aagagccctt cacgacgctg
     5161 cacccgggcg aggtgcgcat gtacgcctgc ggcccaacgg tgtatgacta tgcgcacatc
     5221 gggaacttcc gcacgtttgt ttttcaggac gtcctgcggc gctttctcga gcttcgcggt
     5281 taccgcatga atcaggtcat gaacctgacc gacgtggacg atcgcatcat cgcgaacgcc
     5341 gcggcggccg gtgccagcat ccgcgattac acggaaaagt acgcgcaagc ctttttcgcg
     5401 gactgccgcg ccctgaactt cgagccgccg gaacattgga ttcgcgcgac cgagcacatt
     5461 gaagacatgg ttgtgctgat cgagcggttg cgcgcgaaat cgctgactta tgagagcgag
     5521 ggatcgatct acttccggat cgcaaaattc cccgcctatg gaagactttc caagattgac
     5581 gtgagcggta tccaggcggg cgcgcgcgtg gatgtggacc gctacgagaa ggagagcgcg
     5641 cgcgacttcg cgctgtggaa agcgccgaag ccggaggagc agttctggga gacgcgcatt
     5701 gggcgcgggc gtcctggctg gcacatcgaa tgctcagcga tggcgatgct ctatctgggg
     5761 gaaactctcg acatccactc cggtgggatt gatcttgtct ttccccatca tgagaacgag
     5821 atcgcgcagt cggaggcggc cacgggaaaa cagttcgtgc gctactggct gcatgcggag
     5881 catctcctcg tcgagggcga gaagatgtcc aagtcgctcg gcaacttcta caccttgcgg
     5941 gacctctttg cgaagggcca caagccctcc acgctgcgtt ttctcctggc gagtgttccc
     6001 taccgcaagc agttcaattt cacgtctgaa ggattgcaac aggccgccag ctcggtccag
     6061 cgcgtgcgca acctggccgc gcgcctgaaa gaggagaagt tccccggggg aaaagagacc
     6121 gcgctttctg cgcgcgcgcg caacgcggag gaagagttcg aagctgcgct ggccgacgat
     6181 ctcaacaccg cgcaggcgct ggcagccgtt tttgatctcg tgcgcgatgc aaatatcgcc
     6241 atggacaaag gcgaattcca gaagggcgat gccgctccgg tgcaggcgtt tctcacgaag
     6301 ttcgaccgtg tttttgccgt tctgcgggac gacgatggcg aaaagctgcg cgcgctcggc
     6361 ttcgcgggcg ccgctgaatt gaccgatgcc gaggtcgaga agcaggtagc gcagaggaac
     6421 gaggcgcgca aacggcgcga cttcgccgcc ggggaccgca tccgcaaaga actcgcggac
     6481 cgtggtataa tcctcgagga caccaaagat ggcggtgtcc gctggaagcg caaatgagca
     6541 acctatcaca cagcctccct tatccgtcgg ggcatctccc gccggccctc cagcggccca
     6601 acctgttcgg cgtagactaa ccttcacttc tctgttctcc gctactcccc tggctggagt
     6661 ccgtcagaaa caaaagaact cccgccgcct gatccaaaca accacctgtc cagaaaccgc
     6721 gactgcggtg agaggagaac gagatggcgg tcacagtgga atccaagctc ccccggttga
     6781 ttacggcgct gccgggaccc catgcgaagc gcgtcatcga gtacgacgcg agatttgttt
     6841 cgccgtccta tacgcgcgat tacccgttcg tagccaagcg gggacgcggc gccacgatcg
     6901 aagacgtgga tggcaacact tttctcgatt tcgcggccgg gatcgcagtg tgctcgacgg
     6961 gccactgcca tccccgggtc gtggaagcga ttcagaggca ggccgccgaa ttgatccaca
     7021 tgtccggcac ggatttctat tacgagagca tgccgcaact ggccgagcgg ctcgccagga
     7081 cggtgccggg aagcgaaggc aaacgcgtgt acttcggcaa ttccggaacc gaagcggtgg
     7141 aagcggcgct caagctggcg cgctacgcga cgcggcgcga taaagtgatc gcgttctatg
     7201 gagcgtttca cggccggacc atgggcgcgc tttcgttgac ggcctcaaag tcggttcagc
     7261 ggaaaggctt tggcgctctt ctctccggga tcgagcacat cccctatccc tatgcctatc
     7321 gctgcgcgct ggggcacacg gcggaaactt gcggcgcgga aatcctcgaa atgctcgagc
     7381 gggagatgtt caagcggctc ttcgaccccg aagagatcgc cgcaatcatc atcgagccca
     7441 ttcagggaga gggcggctac gttcccgcgc ccgccttttt tctgcaggaa c
//
LOCUS       DASYVO010000352         1824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_110994, whole genome shotgun sequence.
ACCESSION   DASYVO010000352 DASYVO010000000
VERSION     DASYVO010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110994"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1824
                     /locus_tag="VGR03_11940"
     CDS             <1..>1824
                     /locus_tag="VGR03_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995033.1"
                     /translation="EQLAAHHQPVTAAWKFRYRYDSGVLSIDSGEFETPSSRGNIDGT
                     LGPRNSTLNLRFETGALETYKDFIDGLRGAAPGSAEAAKQISGSVRWDGKIMGPSGGP
                     TFQGHLRGERARYDGVFVDFLEGDLTYSPSKMALEHGHARSGEMEANIEGRLSLKKWS
                     FLPQNDWAAEMNFEKIPAESLEQLLGMPFPVKGSLTGQFHGRGTREQPNVTGLFDLAD
                     GKVYGLSFNRLRGQLNVSPDEVRIADAELRFFPPGKENGRGAGIITGSAGYRYADQTI
                     SAALVGAALPLENFEKLQSDRLPVGGQVSFRLKANGPLRTPLGEGAFRVVDLRIGQVV
                     VGSFDGKLTSDGRAAYLELSSAMATGEVSGGYTLGLADPYPLSGKVSIKNIDLDPFLL
                     AALHLKEFSGHANADGDISVNGLLKQPQSIIVDANLSRLAMNYANVRLENAGPVHFRS
                     TKDSLEIDPVTLRGADTNLQIAGSVQFAGRRAVGLRLNGALDLRLITGFVPNLDARGP
                     AQINASFEGTLDRPRITGRVHIENASARAADFPTGLSAIKGDVVFDATRLYFENMSAE
                     SGGGTLHLSGSVNYAESPLRYDVSVRTDRVRIRYPEGMSWL"
BASE COUNT          372 a          508 c          575 g          369 t
ORIGIN      
        1 cagaacaatt ggccgcgcac catcagccgg tgacagctgc ctggaaattc cgctaccgat
       61 acgattcggg tgtcctctcg atcgactcag gagaattcga aacgccatcg tcgcgcggga
      121 acatcgatgg cactcttgga ccgcgcaatt cgacgttgaa tctgcggttt gaaaccggag
      181 ctctggaaac atacaaagat ttcattgatg gcttgcgtgg agctgcgcct ggttccgcgg
      241 aggctgcgaa acaaatttcg gggagcgtgc ggtgggatgg caagatcatg ggcccctccg
      301 gcggccctac atttcaaggg cacctgcgtg gggagcgcgc gcgttacgac ggcgtcttcg
      361 tggattttct tgaaggtgat ctgacctact cgccttcgaa gatggcgctg gagcacggac
      421 atgcccgcag cggcgagatg gaagcgaata tcgaaggaag actttccctg aagaaatgga
      481 gtttcctgcc gcagaacgac tgggcagctg agatgaattt cgaaaaaatc ccggccgaaa
      541 gtctggaaca actgctcggg atgccttttc cggtgaaggg atcattgacg ggacaatttc
      601 acgggcgggg cacccgggag cagcccaacg ttacaggcct gttcgatctc gccgatggca
      661 aagtgtatgg actttccttc aatcgtctcc gtgggcaact gaacgtctcg ccggatgagg
      721 tgcgtatcgc ggacgcggag ctgcgattct tcccgccagg aaaagaaaat gggcgcggcg
      781 cgggaattat caccggatcg gctggttacc gctacgcgga tcagacgatt tccgctgccc
      841 tcgtgggcgc ggcgcttccg ctggaaaatt ttgaaaaatt gcagtcggac cgcctgcctg
      901 tgggagggca ggtgagtttc cggctgaagg ccaacggtcc gttgagaaca cctctcggcg
      961 agggggcatt tcgagtcgtg gacctccgaa ttggccaggt ggtcgtaggc agtttcgacg
     1021 gcaaactgac gtcagatggc cgcgcggcat atctggaatt gagctccgcc atggccacgg
     1081 gggaggtttc cggtggctac acactcggac ttgccgatcc atacccgctg agcggaaaag
     1141 tttcgatcaa gaacattgac ctggacccct ttctgcttgc cgctttgcac ctgaaagagt
     1201 tcagcggcca tgcgaacgcg gatggcgata tttcggtaaa tggtttgctg aagcagccgc
     1261 agagcatcat cgtggatgcg aatctctcgc gcctggcgat gaactacgcc aacgtgcggc
     1321 tggaaaacgc gggcccagta catttccgat cgacgaagga cagcctggag atcgacccgg
     1381 tcacgctgcg gggagcggac actaatctgc agattgcggg ttcggtgcag tttgccgggc
     1441 gccgcgccgt cggattgcgc ctcaatggcg ctttggacct gcggctcatc accggctttg
     1501 tgccgaatct cgacgcacgg ggtccggcgc aaatcaacgc ctctttcgaa gggacgctgg
     1561 accggccgcg catcaccggc cgcgtgcaca tcgaaaacgc ttccgcccgc gctgcggatt
     1621 ttcccacggg actcagtgcc atcaagggcg acgtggtatt cgatgccaca cgcctgtact
     1681 tcgagaatat gagcgcggaa tccggcggcg gcacgctgca tctttccggc agcgtgaatt
     1741 acgcggagag tccgcttcgc tacgacgtca gcgttcgtac cgatcgcgtg cgcatccgct
     1801 atccggaggg catgagttgg cttg
//
LOCUS       DASYVO010000353         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_146570, whole genome shotgun sequence.
ACCESSION   DASYVO010000353 DASYVO010000000
VERSION     DASYVO010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146570"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VGR03_11945"
     CDS             <1..555
                     /locus_tag="VGR03_11945"
                     /inference="COORDINATES: protein motif:HMM:NF037850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995034.1"
                     /translation="DESIGFTKAVVCSNCDHLKFYIADKLVAEADPNKTEFAHLRYSP
                     FVVDLGRAVGHWGDLRIDGYLQGKQVISKTLSGRGIDQKFTLLPDDTHLNGDGADTTR
                     VVLRVTDEFGAIRPFANDAIKLELEGPAEIIGDNPFALIGGTGAIWIRAKEQPGTVKL
                     TATHPQLGPQQLQFEIAPAPPEVA"
     gene            713..789
                     /locus_tag="VGR03_11950"
     tRNA            713..789
                     /locus_tag="VGR03_11950"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:747..749,aa:Arg,seq:tcg)
     gene            complement(859..1614)
                     /locus_tag="VGR03_11955"
     CDS             complement(859..1614)
                     /locus_tag="VGR03_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995035.1"
                     /translation="MVTAVVRPSSPTKPAIGLPGRRYDHLFFLAMALLMLGTVFVGFA
                     HTYYLAGVFHAPLPSLIIHLHGAAFSCWILLLVTQTSLVSAGRVEIHRRLGIAGFLLA
                     CLMVILGVLAATDSLVRESGPPGRDPKFFYIVPLTDMLVFGALVFFAFRARSNPPAHK
                     RLIFVATTTLLIAAFGRWPIDMIRGNPAMAGLASYIFVLFLVGYDLWSTRKVHRATIW
                     AGALLIFVQQIRIPIGKTAAWHAFASWAQTHAR"
     gene            1859..2323
                     /locus_tag="VGR03_11960"
     CDS             1859..2323
                     /locus_tag="VGR03_11960"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEV1995036.1"
                     /translation="MFKLLFAMLGFALTAYSADLPTRKYVNLAAIKTMVAAAEAEAKR
                     LHVEVTICIVDESGNLLFLQKADAASLNTIVWAQKKARHAAFYRSPSKDAADNVKKGN
                     VEAVAFPDYFPNQGGLPIQVDGQTIGGIAASGAKSEIDEAIAQAALDALFKK"
BASE COUNT          529 a          675 c          686 g          452 t
ORIGIN      
        1 gatgaatcca tcgggttcac caaggccgtc gtctgctcga actgcgacca cctgaaattt
       61 tacattgcgg acaaactggt agcggaggcg gatccgaaca agaccgagtt tgcccacctg
      121 cgctattccc cgttcgttgt cgatttgggg agagccgtcg gccactgggg agacttgcgt
      181 atcgatggct acctgcaggg aaagcaagtg atttccaaaa ctctttccgg ccggggcatc
      241 gaccagaaat ttaccctgct gcccgacgac acgcatctga acggcgatgg tgctgacacg
      301 acgcgcgttg tgttgcgcgt aaccgatgag tttggcgcca tccggccctt cgccaacgac
      361 gccatcaagc tggaactgga aggccctgcc gaaatcattg gcgacaatcc tttcgcctta
      421 attggcggca ctggcgccat ctggattcgt gcgaaagaac aacccggtac ggtgaagctc
      481 acagcaacgc acccgcaact ggggccgcag caactgcaat tcgaaatcgc tccggctcct
      541 ccagaagtcg cctagccgcc gcgcgagcat ctatcagcta aatgcggggg caaatgtcgg
      601 ggtaaatgta ggggcgcact ttagtgcgtc cctctttctg ttgattctgg ttcttagctt
      661 cgcgcaggag ccccccaccc aaatcccgca tcacttgcta taatgcctct cagcgcccgt
      721 agctcagctg gatagagcat ctggcttcga accagagggt cgggagttcg agtctctccg
      781 ggcgcgccac ctgctcttgt agttccctat ctgcgacttc cgcgcggtcg cttctcgatg
      841 atgtaaggaa aaattgggct agcgggcgtg ggtttgcgcc caactggcga aggcgtgcca
      901 tgctgctgtt ttgccgatgg gtatccttat ctgttgcacg aagatgagga gagcgcccgc
      961 ccagatcgta gcgcgatgca ccttgcgcgt ggaccacagg tcgtagccta ccaggaacag
     1021 tacaaatatg taggaggcca gccctgccat ggccgggttt cctcgaatca tatcgattgg
     1081 ccaacgtccg aaggcggcga tcaacagcgt ggtcgtcgcg acgaagatga ggcgcttatg
     1141 cgctggggga ttggagcggg cgcggaaagc gaaaaagacg agcgccccaa aaaccagcat
     1201 gtccgtaagg ggaacgatgt agaaaaattt tggatccctg cccggtgggc cggattcccg
     1261 aacgagcgag tcggtggccg ccaaaacgcc gaggataacc atgagacacg ccagcaggaa
     1321 gccggcaatg ccgaggcggc gatgaatttc gacgcggccg gcggagacca gggaagtttg
     1381 cgtcacgagc aagaggatcc aacaggaaaa tgccgccccg tgcaggtgaa tgatcaggct
     1441 gggcagcggc gcgtggaaca ccccggcgag atagtaggtg tgcgcaaagc ccacgaaaac
     1501 ggttcccagc atcagcaggg ccatggccaa gaaaaataga tggtcgtaac ggcggcccgg
     1561 caagccgatc gcaggcttcg tgggactgct tggacgtact acggcggtga ccatcgttcc
     1621 tctttcaccg actgtcttga ccgagatttc ttggagcccc ggggagtgga aaccagtgta
     1681 gggcatgctg tgattggata actagcgaat actggtgttt gcaccggcga taggaattac
     1741 atcttccaag aaccagtttg catccaactg cctgatcggt tcggcctgtc atcccaccat
     1801 cccggcaacg ccacatttca tctggaatat ttgaatgcca agaggtagca tcctgcacat
     1861 gttcaaacta ctttttgcta tgctcggttt cgccctgaca gcgtactcgg ccgatctccc
     1921 caccaggaaa tatgtgaacc tggcggcaat caagacgatg gtcgccgctg ctgaagcgga
     1981 agccaaaaga ctgcatgttg aagtcaccat ctgcatcgtg gacgagagcg gcaatctgct
     2041 ctttcttcag aaagccgatg ccgcctccct caacaccatc gtgtgggccc agaagaaagc
     2101 ccggcacgcg gccttctaca ggagtccctc gaaagacgcc gccgacaacg tgaaaaaggg
     2161 caacgtggag gccgtggctt ttccggacta ctttcccaac caaggcgggc tgcccatcca
     2221 ggtggacggc cagaccatcg gcggcattgc cgccagcggc gccaaatcgg agatcgacga
     2281 agccatcgca caagcggcgc tcgacgctct cttcaagaag tgatggcggc gaagctccgt
     2341 cc
//
LOCUS       DASYVO010000354         1521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_248752, whole genome shotgun sequence.
ACCESSION   DASYVO010000354 DASYVO010000000
VERSION     DASYVO010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248752"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VGR03_11965"
     CDS             <1..824
                     /locus_tag="VGR03_11965"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEV1995037.1"
                     /translation="KAWGVRATAADTLGQLDGASASKLLLAAVDSHDGPWVRSRAVSA
                     LGNFKDNAAVAARLDAIAKQDDSYRARAAALQALGRLKGPNAFATLDAAVAADSPDGF
                     LRNAALRSLGSLGDDKAVPLLLQWSAAGKPIDSRTAAINSLARLQKDNKDITKQIAAY
                     LTEPHFTVRMAAIYALGGRGDATAIPALEALLKSDDLSIEMAPMIKGQIARLKKPADG
                     KPGGNPGGGDADEESVEAGGEKLTTEQRLEKLEHLLQEMSERLKSMEKRLPPPKQ"
     gene            complement(943..>1521)
                     /locus_tag="VGR03_11970"
     CDS             complement(943..>1521)
                     /locus_tag="VGR03_11970"
                     /inference="COORDINATES: protein motif:HMM:NF017013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HEV1995038.1"
                     /translation="LDEGKMPETPKADAAAFRNSHFSLLLGMDDLFHPAHHAAEAKGF
                     IACCDNTTDDWPVEAAAEFLLEQLEELRKNHAGQRVALIVDGEVSSPVTGNGIGGRNS
                     AFVLACVEKIAGKKIAVLSAGTDGIDGNSSAAGAVADGETLTRARAMGLDPADAFRRS
                     DAFTFFSRLEDAIVTGPTGNNLRDLRILIAEP"
BASE COUNT          307 a          507 c          407 g          300 t
ORIGIN      
        1 ataaagcctg gggagttcgc gccaccgccg ccgacacgct cggccagttg gacggcgctt
       61 ccgcatccaa gctgctcctc gccgcagtgg attcgcatga tggaccctgg gtccgaagcc
      121 gcgccgttag cgcgctgggc aacttcaaag acaacgcggc cgttgcggcg aggctcgatg
      181 ccatcgcgaa acaggacgat tcttatcgtg cccgcgccgc ggcgttgcaa gcacttggcc
      241 gccttaaggg gcccaatgct ttcgcgacgc tcgatgccgc cgtcgccgcc gattcgccgg
      301 acggcttcct acgcaacgcc gctcttcgct cgctcggttc gctgggggac gacaaggccg
      361 tgccgcttct attgcagtgg tcggccgccg gcaagcccat cgattcgcgc accgcggcca
      421 tcaacagcct cgcgcgtctg cagaaagaca acaaggacat cacgaaacag attgccgctt
      481 atctcactga accgcatttc accgtgcgca tggccgcgat ctatgcactg ggtggacgtg
      541 gcgatgccac tgccattccc gcgctggaag ctctgctgaa gagcgacgac ctcagcatcg
      601 aaatggcgcc catgattaaa ggacaaatcg cacgcctgaa gaaacccgcg gacggaaaac
      661 caggtggcaa tcccgggggc ggagatgccg acgaagagtc cgtcgaggcc gggggtgaaa
      721 agctcacgac cgaacaacgg ctcgaaaagc tcgaacacct gctccaggaa atgagcgagc
      781 ggctgaagtc catggagaag cgcctcccgc ccccgaagca gtaagtagct gatgtttcag
      841 tgtaggggcg gacttcagtc cgccctctct tctctgtggc ctctgtggcc tctgtgttct
      901 ccgtgacctc tgtgctaaat ctttcttttc aaattcttcg tactaaggtt ccgcaatcaa
      961 tatccgcaga tcgcgcagat tgttccccgt cggtccggta acaatcgcat cctccaacct
     1021 cgaaaaaaac gtgaacgcgt cgcttcgccg gaacgcatcc gccggatcga gccccatcgc
     1081 gcgcgcccgc gtcaacgtct ccccgtccgc caccgctccg gcagcggaac tattgccatc
     1141 gattccatca gtgccggcac tgagcactgc aatctttttt cctgcgattt tttccacgca
     1201 ggccaacaca aacgccgaat tcctccctcc gattccattt ccggtcacgg gtgagctcac
     1261 ctcgccatcc acgatcagcg cgacccgctg ccccgcgtga ttcttccgca gttcctccaa
     1321 ctgttccagt aaaaactccg ctgccgcctc aaccggccag tcatcggtag tgttgtcaca
     1381 acaagcgatg aaccctttcg cttccgccgc gtgatgcgct ggatgaaaca ggtcatccat
     1441 cccaagcagc agcgaaaaat gtgagtttcg aaatgctgcg gcgtcggctt tcggtgtctc
     1501 aggcattttc ccttcgtcaa g
//
LOCUS       DASYVO010000355        11335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_264081, whole genome shotgun sequence.
ACCESSION   DASYVO010000355 DASYVO010000000
VERSION     DASYVO010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 11335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11335
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264081"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..668)
                     /locus_tag="VGR03_11975"
     CDS             complement(255..668)
                     /locus_tag="VGR03_11975"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEV1995039.1"
                     /translation="MAWMWPASSKNGTSEEWTGFIDQGVTLEGTLTVTGTFRIDGNVK
                     GNIISEQTVILGEGAKVEGQIEGNRVVISGRFDGVIFAKGRVEIEAKGVVTGEVHSPC
                     MVIDPGGIFDGRCHMLGSSETASTVTIPIRAVGNG"
     gene            complement(799..1719)
                     /locus_tag="VGR03_11980"
     CDS             complement(799..1719)
                     /locus_tag="VGR03_11980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV1995040.1"
                     /translation="MPRNALGRGLGALIREPEPKTSPEPTPAQPHATTTSGAASAPAR
                     EAVHPGPQQVDIDLIEPSPYQPRTRFREEALDELARSIQTSGIIQPLVVRPIGSRFQL
                     IAGERRWRAAQRAGLTKVPVIVRQVADDLALEMTLVENIQREDLNAIEAARAFVRLMD
                     EAQLTQEAVAERTGKDRATVANSIRLLKLEPTIQEWIEEGKLTAGHGRALLAVPDSQL
                     RMRYAQRAARGGLTVRQIERLASRRSRGTNAPTEIHLDANIRSALDELQRHLGTKVLL
                     HQKTKMRPGQLVLEFYDDAQLMGLYDRLMK"
     gene            complement(1667..2473)
                     /locus_tag="VGR03_11985"
     CDS             complement(1667..2473)
                     /locus_tag="VGR03_11985"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEV1995041.1"
                     /translation="MARIIAVANQKGGVGKTTTAVNLAACLAAAEQPTLLIDCDSQAN
                     STAAIGFPKDPSRRTLYHTLILGEPFDKILQKSQVEGLDVVPADKNLAGAAVELVNAD
                     NREYKLQTALKPMHDKYRFIILDCPPALDLLTLNALVAASAVLIPIQCEYLALEGVSE
                     LLDTLMRIRRTLNPSLEIEGILLTMYDERTTLSRQVATDLRSFFGTQVFQSVIPRNVR
                     LAEAPSFGKPIIFYDIHSKGAEGYTQLAQEVIANAEKRAGTGARSAHSGA"
     gene            complement(2807..3472)
                     /gene="rsmG"
                     /locus_tag="VGR03_11990"
     CDS             complement(2807..3472)
                     /gene="rsmG"
                     /locus_tag="VGR03_11990"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV1995042.1"
                     /translation="MPTNSPAPPSIGTIQRALGEFQLTATDEQVLQIQQYIKILLLWN
                     EKVNLTAIRDPLEILYRHFCESMYASVAVPVKNGRLADVGSGGGFPGLPLKIIRPDLR
                     VFLIESNIRKVTFLAEVTRELGLKDVQVLARRYEELGEEVAPLDYACSRALGEFPAFL
                     DWARSEQIAAKQVILWIGARDLPEIEKIRTWDWREPIQVPHSLRRLLLVGTRKPSSVG
                     AST"
     gene            complement(3499..4215)
                     /locus_tag="VGR03_11995"
     CDS             complement(3499..4215)
                     /locus_tag="VGR03_11995"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEV1995043.1"
                     /translation="MPWDIWLIFFVLGVILPWRGRVRMKKLLAMPHFSTMERLTLYAS
                     TIAFQWFAVAVVAWRAWAHGFTACQLGLTIHNRSRILVASIVGAAMIAALQWLNLRRV
                     GRVPIEARGSLQALAERILPQSTVELLPYLALAITAGICEEFLYRGFAMAVLVHVGLQ
                     TWAVVLLSSILFGLAHSYQGRGGIVMTLLIGIILGTGRIAYDSLVPAIFWHSAVDVVA
                     GTAGPRYLTRRWAAGPEKSA"
     gene            4330..5409
                     /gene="selD"
                     /locus_tag="VGR03_12000"
     CDS             4330..5409
                     /gene="selD"
                     /locus_tag="VGR03_12000"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEV1995044.1"
                     /translation="MTQTVKLTSMAKAAGCAAKLNPATLDAVLRKLPRQTDPNVLVGF
                     DTNDDAGIYLLGDGLALVQTVDFFTPIVDDPELFGQIAAANALSDVYAMGGRPVSALS
                     IVGFPEKSDPAILEQIIRGGLSKMNEAKCSVIGGHSIRNEDMLFGYAVTGVINPNRVW
                     RNVGARSGDVLLFTKPLGTGVITTALKKDRATPESLAAAVTSMTTLNRDAAAALQEID
                     EKAGGAKPIHAVTDVTGFSLLGHAREMALGDPTHGMESVSFEIDHRAFAYLPGAVEAA
                     REGHLSGGLKNNRAFIGDCAAFDASVPPEYQDLLFDPQTSGGLLIAISPESAGAAISA
                     LDRHGVSARRIGKVIAKTNPLLLVR"
     gene            5447..6640
                     /locus_tag="VGR03_12005"
     CDS             5447..6640
                     /locus_tag="VGR03_12005"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEV1995045.1"
                     /translation="MDTITHGIAGALIGKAVFRGEDLFASHPMNRGRIITWSLMLGAI
                     FPDSDVIRDFFSSDKLLIVTWHRSLTHSLVLLPLWALLLAGITHAFAGWRKWEAPSFA
                     ALSAIYAAGILSHILLDLVTTFGTMIWSPLEWSRPAWDLIFIVDFTLTAILLVPQLLA
                     WVYAHPEKVKRRAVGMWLVFLPAPFLIARIGEIVGAPISDRVVLSAMVIFAALFLLPA
                     LLGWGVKIQHDTWNRAGFAAALIYVACTFYAHHVALSRIQKFAELYPLQVASIGALPL
                     PPSLWHWDGLVRTERGVYELRMDLSDKPASDTELLTREHRYYPDAPPNAYIEVAKRLP
                     EVQKVLWFSRFPVTRFHKEGDVAVVEISDIRFVQTRRDRPASFTYRVRFGSAGIVLSQ
                     GWVTR"
     gene            complement(6683..7210)
                     /locus_tag="VGR03_12010"
     CDS             complement(6683..7210)
                     /locus_tag="VGR03_12010"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEV1995046.1"
                     /translation="MAQELIRDGKLDRQAAAEALRQFLENLVRVAGLELKVNVCPVAP
                     EGESSGGAEVLADLDGKDKEILLERSAEVLKAFEHLAFRALRLEPAYHEKIHIDCGGY
                     RALRFEELRMTARVAAERVQSSRQPFRLNPMSSRERRIVHLALKDMTGVRTESAGVGE
                     DRQVVIHPADSKPSL"
     gene            complement(7259..9016)
                     /gene="yidC"
                     /locus_tag="VGR03_12015"
     CDS             complement(7259..9016)
                     /gene="yidC"
                     /locus_tag="VGR03_12015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1995047.1"
                     /translation="MSAPGQKDKFTPELRILVASLLSFGVIILWAKFFGPKPPVQPTQ
                     ANRPAQTAPATPGPATSPAANASHATAVPATIAPATATSVTVKNDSQERTIVVENSLY
                     RVEISNHGAVVKSWQLKHYKDDAKPQRVLDVVHPDASQQTGGWPFSVVLDDEQLQNAA
                     NDGLYQLSSPATPLQAPADVEFTWSDGHLEVTKHFRFDHTYVVRVETSAKLNGSPITA
                     GLGWLGGFGDLTVTNPAPVETVSTFYSEGGKVSTFLHKKLDGLEKWGPGLWQGGKEFV
                     GIEDRYFAATFLPASGATSNALETRYWKASRTVKVDGQDALEPVPQVAAATTAQPLTL
                     RVYVGPKDYDDLKKMSPPLHGLINFGWLEFIAEPLFHGLKWLHNYVPNWGWAIVVLTL
                     VINMLLFPLRISSYKTTLKMQRVAPEIKAIQDKYKKYSMRDPRKAEMNKEVMAIYSRE
                     GINPVGGCFQMFLQMPVWFGLNTALRYSIEMRQATWLWVLDLSAKDPYYILPVLMGAS
                     MYLVSKMTPMTSTDPQQQMMMKIMPITMAGIFMISPISSGLAVYILTSSMVGIVQQWY
                     LNRTHPVAAPAKPVRGKKS"
     gene            complement(9013..9384)
                     /gene="yidD"
                     /locus_tag="VGR03_12020"
     CDS             complement(9013..9384)
                     /gene="yidD"
                     /locus_tag="VGR03_12020"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV1995048.1"
                     /translation="MDAGEEGGLKSAPTQVSPTQVAATQIEDRSIAVRAALLALRFYK
                     AYLSILFAGSCRFEPTCSRYAYEAIERFGVTRGVWLELKRLLRCHPLSRKFGYDPVPE
                     KWEEMPTSSTAAKVLKTEAHS"
     gene            complement(9417..9776)
                     /gene="rnpA"
                     /locus_tag="VGR03_12025"
     CDS             complement(9417..9776)
                     /gene="rnpA"
                     /locus_tag="VGR03_12025"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV1995049.1"
                     /translation="MPTASSGAQGNLFPREARLVRRGEFDAVYRAGKRRSSSHFTVFF
                     RANELPQSRFGFSIKKALGGAVVRNRIRRRLREVVRCHRLEIPAGWDIVIHPKTSVAR
                     AEFAALTADLLRLLKSA"
     gene            complement(9790..9930)
                     /gene="rpmH"
                     /locus_tag="VGR03_12030"
     CDS             complement(9790..9930)
                     /gene="rpmH"
                     /locus_tag="VGR03_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV1995050.1"
                     /translation="MPKRTFQPRKRRRAKTHGFRVRMKTKGGRKVLAARRKKGRHRLT
                     PE"
     gene            complement(10263..10697)
                     /locus_tag="VGR03_12035"
     CDS             complement(10263..10697)
                     /locus_tag="VGR03_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995051.1"
                     /translation="MSHGSDHASMGSSAKGSASSPTSVLDRNTKLDSKLTSKLQSKGL
                     LPAGTDLKDACAGFRNLGQCMAAIHVSHNLKILFACLQADMTGTAPAQVSTCPTGTGS
                     SKMSLGKSIQTLSPNANAKAEAKNATKQADADVKEAESETNS"
     gene            complement(10882..>11335)
                     /locus_tag="VGR03_12040"
     CDS             complement(10882..>11335)
                     /locus_tag="VGR03_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995052.1"
                     /translation="FGRWTPFASAGVGNSLLDTRYWHRPFLTLGPVAHFEGGTSFDLG
                     HSLTLSASAYDVAPWGTQKVYSRLVTKGAGGQGGTPKHGRVFQNNALTTGGAAIDRDN
                     GYNADLDFSPLKYVDFDLSYSHSVHFQLDTVSFSIAFNLTPLLHRGSR"
BASE COUNT         2321 a         3392 c         3192 g         2430 t
ORIGIN      
        1 cgcccgtccg acgcattggt ctattacaag gccgcgaaag tccccaaagt gccccacctt
       61 gattgggaat caaacatcca tgcgggaatt caagctgcgg aaaaagccgc aggcgtctcg
      121 ttcgtgccga ctgcgaattc ctaggctcag tcttgcggaa taagaacaat ctggaatgga
      181 cagcgtagag aaggtttcac tttactcact gaacgggcag tcctgcgatt aactagcgga
      241 aaatccgaaa agcatcagcc gttgccgacg gcacggattg gaatcgtcac ggttgaagcc
      301 gtctccgaag atcccagcat gtggcagcgg ccatcgaaga ttccgcccgg atcgatgacc
      361 atgcacggag agtgcacctc tccagtcacc acgcccttgg cctcaatctc cacgcgcccc
      421 ttcgcaaaaa ttacgccatc gaagcggccg gagattacca cgcgattgcc ttcgatctgt
      481 ccttccacct ttgcgccttc gccgaggatt acagtctgtt cggaaatgat attgcccttc
      541 acgttgccat caatgcggaa cgtgccggtg accgtcagcg tgccctcgag cgttacgccc
      601 tggtcgatga accctgtcca ttcttcactg gtgccgttct tcgagctagc gggccacatc
      661 catgccatat caaaaccccc atctagtgac tgttctactt tagctgcgca cgcacataag
      721 tcaactcagc tgagcaagcg ggttgtcgaa tgaggcgact gaaccaacgg gcagtgtttg
      781 ctggcggagc ccgcgaagct atttcatcaa acgatcataa agacccatca gttgtgcgtc
      841 gtcataaaac tcaagcacaa gctgccccgg acgcattttc gttttctgat ggagaagcac
      901 cttggtgccg aggtgccgtt gaagctcatc caacgccgac cggatgttcg cgtccaagtg
      961 aatttcggtt ggcgcattcg tgccgcgcga ccgccgcgag gctagtcgtt cgatctggcg
     1021 cacggttaac ccgccgcgtg ccgcccgttg agcgtagcgc atccgcaatt gcgaatctgg
     1081 aactgccagc aaggcgcggc cgtgccccgc tgtcagtttc ccttcttcaa tccattcctg
     1141 aatcgttggc tccagtttca acaggcgaat cgaattcgcc accgtcgcgc ggtccttgcc
     1201 ggtccgctcg gcaaccgctt cctgggttaa ctgcgcctcg tccatcaagc gtacaaacgc
     1261 acgcgcggct tcaatggcgt tcagatcttc acgctggatg ttctcaacca gcgtcatctc
     1321 caacgcgaga tcatccgcca cttggcggac gatcaccggg actttcgtca agccggcgcg
     1381 ctgcgctgcg cgccagcggc gctctcctgc aatcaactga aaccgcgagc cgatcggtcg
     1441 aacgaccaag ggttggataa tcccgctggt ctgaatcgaa cgcgccagtt cgtcgagagc
     1501 ttcttcccgg aagcgcgtac gaggctggta aggactgggc tcgatcaggt cgatgtcaac
     1561 ctgttgaggg ccgggatgca cggcctcgcg agccggtgca cttgcggcac cgctggtggt
     1621 tgttgcatga ggctgtgcgg gtgtgggttc tggcgaagtc ttgggctcag gctcccgaat
     1681 gagcgctccg agcccccgtc ccagcgcgtt tctcggcatt ggcaatgacc tcctgggcga
     1741 gctgcgtgta gccttctgcg cccttgctgt gaatgtcgta aaaaatgatc ggtttaccga
     1801 aacttggtgc ttccgccagg cgtacattgc gcggaatcac actttgaaag acttgtgttc
     1861 cgaagaaact gcgcaaatcc gtggcaacct gacgggaaag agtcgtccgc tcatcataca
     1921 tcgtgagcag gattccttcg atttcgaggc ttggattcaa ggtgcgccgg atacgcatca
     1981 gggtatcgag caactcggaa acgccttcca gcgcgaggta ttcacactgg atggggatca
     2041 ggacagcgct tgccgccacc agcgcattca gggtcaacaa atccagggct gggggacagt
     2101 ccaggatgat aaagcgatat ttatcatgca tgggcttcag tgcagtttgc agcttatact
     2161 cgcgattatc cgcatttacg agctcaacag ccgcgccagc gaggtttttg tcggcgggga
     2221 cgacatctaa cccttccact tgcgattttt ggaggatttt gtcgaaaggt tcgcccagga
     2281 tcaaggtgtg atagagggtt ctgcgagaag gatctttcgg aaagccaatg gccgcagtcg
     2341 agtttgcttg ggaatcgcag tcaatgagga gagtaggctg ttcggcggca gcgaggcagg
     2401 cggccaggtt caccgccgtc gtggtctttc ccaccccacc cttttgatta gctaccgcaa
     2461 tgatccgggc caaacgcatc ctcgcgagcg aaaatttagc aaagactgcg taaggatgca
     2521 agatgtttca cgtggaacat catttagcgg tggaaatcgt ggttaggagg gcggattgtt
     2581 ccatgtggaa cattccccaa cccttcggga ccggcagata ggacttgttc cacgtggaac
     2641 gaaagcccgc gatgtatcgg tcttttcccc cattaaatcg ttccacgtga aacattgggt
     2701 cctttgacag aggcgtatgt tccacgtgga acgttgatcg gctgcaattg cctcgatgac
     2761 tatcaatccg cgggatttct agagaaatag cagaagtccc ttactttcaa gtcgatgcgc
     2821 cgacgcttga gggtttcctc gtgcccacca ggaggaggcg tcgcaaggaa tgaggtactt
     2881 ggatgggttc gcgccagtcc caagtgcgga ttttttcgat ctcggggagg tcgcgagccc
     2941 caatccagag aatgacttgt tttgctgcta tctgttccga ccttgcccag tcgagaaaag
     3001 cagggaactc accgagcgcc cgcgagcaag cataatccag tggtgcaacc tcctcgccaa
     3061 gctcttcata gcgccgcgca agaacctgta cgtccttaag tcccaattca cgagtgactt
     3121 ccgcgagaaa tgtgactttc ctgatgttgg attcgatcag aaatacccgc aaatccggcc
     3181 ggatgatctt gaggggcaaa cccgggaatc cgcctcccga cccaacgtcg gcgagccgac
     3241 cgtttttcac aggtacagcc acggacgcgt acatactttc gcaaaaatga cgataaagta
     3301 tctccagtgg gtcgcgaatc gcggtcagat ttaccttctc attccacagt aacaaaatct
     3361 ttatatattg ttgaatttga agcacttgtt cgtctgtcgc agtgagctga aactcgccga
     3421 gtgcgcgttg gatggtgcca atggacggag gggctgggct atttgttggc ataatagtaa
     3481 attaaaaatc accgcaattc aagcgctctt ctccgggccg gcggcccatc ttcgcgtcag
     3541 gtatcttggt cccgcggtcc cggcgaccac atccactgcg ctatgccaga aaatcgcagg
     3601 caccaggcta tcgtaggcga tccggcctgt tcccagaata ataccgatca acagtgtcat
     3661 cacaatccca ccgcgcccct ggtaactatg tgccagcccg aagagaatgg aagaaagcag
     3721 cacgaccgcc caagtttgca atcctacatg gacgaggacg gccatggcga acccgcgata
     3781 gagaaactcc tcgcagattc ctgctgtaat cgcgagcgcg agatagggaa gcaactccac
     3841 cgtggattgc ggcaagatcc gttccgcaag ggcctgaaga gagccccgcg cttcgatggg
     3901 aactctaccc acgcgccgca ggttcagcca ctggagagcc gcaatcatcg cggcgccgac
     3961 aatcgaggca acaagaattc gggacctgtt atggatcgtc aaaccaagct ggcaggccgt
     4021 gaatccgtgc gcccaggcac gccacgccac caccgcgacg gcaaaccact gaaaggcgat
     4081 agtcgaagcg taaagggtga gccgctccat cgtgctgaag tgtggcatcg ccagcaattt
     4141 cttcatgcgc actcgcccgc gccacggaag aatcacaccg agcacgaaaa agatgagcca
     4201 gatgtcccag ggcatccgcg aataagaaca ctttccgcct ccgccgcgca agtatctaga
     4261 gttctattgc acacgagcac gaaccacggc acaccgctat aatcgaaagg ctttagagcg
     4321 aggtgcggct tgacccaaac cgtgaaactc acgtcgatgg caaaagcggc gggctgcgct
     4381 gcgaaactca atccggcgac gctggatgca gtcctgcgca aattgccgcg tcagaccgat
     4441 ccaaacgtcc ttgtgggatt cgacaccaac gacgatgccg gtatttattt gcttggcgat
     4501 ggcctcgcgc tcgtgcaaac cgtggatttc ttcacgccga tcgtagacga tccagaactc
     4561 tttgggcaga tcgctgcggc caatgcgttg agcgatgtct acgccatggg cggccgtccg
     4621 gtctccgcgt tgtccattgt cggttttccg gaaaagagcg accctgcgat cctcgagcag
     4681 attattcgcg gcggcttgtc caaaatgaac gaagcgaaat gcagcgtcat cggcggccac
     4741 tcgattcgaa atgaagacat gctctttggc tacgcggtca ccggcgttat caatccaaac
     4801 agagtttggc gaaatgtcgg cgcgcgcagc ggtgatgtcc tgcttttcac caaaccgctg
     4861 ggcacgggcg tcatcacaac ggcgctgaag aaggaccggg caacgccgga atctttggcg
     4921 gccgcggtta cctccatgac gactctgaat cgcgatgccg ccgccgctct gcaagagatt
     4981 gatgaaaaag ccggtggtgc gaaacctatc cacgcggtaa cggatgttac tggcttcagc
     5041 cttctcggcc acgcacgcga aatggcgctc ggcgatccga cacacggcat ggaatcagtt
     5101 tccttcgaaa tcgatcaccg cgcattcgcc tatctccccg gcgccgtcga agccgctcgc
     5161 gaaggccatc tctctggcgg cttgaaaaac aaccgcgctt tcattggcga ttgcgcagcc
     5221 ttcgacgcca gcgttccgcc cgaatatcag gatttgctct tcgatcccca aacttccggc
     5281 ggcctgctga ttgcgatctc gcccgaatcc gccggcgccg ccatctctgc gctcgatcgg
     5341 catggcgtct ccgcgcgccg catcggtaaa gtcatcgcca agacaaatcc gctcctcctc
     5401 gtccgttaga atgtcacgtc cggtgcgcat cggatagact cgaacgatgg acacgatcac
     5461 acacggcatt gccggcgcat tgatcggaaa agcggtcttc cgcggcgagg acctgtttgc
     5521 ctcgcacccc atgaaccgcg ggcgcatcat cacgtggtcg ctgatgctcg gcgccatttt
     5581 tccggattcc gatgtaatcc gggacttctt ttccagcgat aagcttctca tcgttacctg
     5641 gcatcgcagc ctgacgcact cgcttgtact cctgccactc tgggcgctgc tgctcgcggg
     5701 aatcacgcac gcattcgcag gctggcgaaa atgggaagcg ccctcctttg cggcgttgag
     5761 cgcgatctac gcggcgggca tcctcagcca tattttgctc gacctggtca ccacgtttgg
     5821 caccatgatc tggtcgccgc tcgaatggtc gcgtcccgcc tgggatctca tcttcatcgt
     5881 ggatttcacg ctgaccgcga ttcttctcgt cccgcaattg ctcgcctggg tgtatgccca
     5941 tcccgaaaaa gtgaagcgcc gcgccgttgg gatgtggctg gtgttcctgc cggccccctt
     6001 tctcatcgcc agaattggtg aaattgtcgg cgcgccgatt tccgatcgag tcgttttgag
     6061 cgccatggtc atttttgccg cgctcttcct cctgcccgcg ctcctcggtt ggggcgtgaa
     6121 gatccagcac gacacttgga atcgcgctgg ttttgccgcg gcattgatct atgtggcatg
     6181 cacgttctac gcgcaccacg tcgcgctctc gcgcattcag aagttcgcgg aactataccc
     6241 cttgcaggtt gcatcgatcg gcgcgttacc gcttccgcca tcgctctggc actgggacgg
     6301 gctggttcga acggagcgcg gcgtctacga actgcgcatg gacctcagcg acaaaccggc
     6361 aagcgacacg gaattactta cgcgcgagca ccgctactac ccagacgcac cgccgaacgc
     6421 gtatatcgag gttgccaaac gcctgcccga agtgcagaaa gttctgtggt tctcgcgatt
     6481 tcctgtgacg cgcttccaca aggaaggcga tgtagcggta gtggaaatct ccgatatccg
     6541 ttttgtgcaa acgcgccgtg accgcccagc gtccttcacg tatcgcgtgc gcttcggctc
     6601 agccggaatt gtgctctcgc aaggctgggt cacgcgctaa gaccgccata cttgtctgtt
     6661 attttcgtgc ggcaattcag ggctacaggg aaggtttgct gtccgccgga tggatgacga
     6721 cctggcggtc ttcgcccaca ccggcgctct ccgtgcgcac tccggtcata tccttcaacg
     6781 ccagatgcac gatgcggcgc tcgcgcgagg acatgggatt cagccggaac ggctggcgag
     6841 aactctgcac gcgttcggcg gcaacgcgcg ccgtcatacg caattcttca aaacgcagcg
     6901 cgcgataacc gccgcaatcg atgtggatct tttcatggta ggcaggttcc agccgcaggg
     6961 ctcggaaggc gaggtgttcg aacgctttca aaacttccgc gctgcgctcc aggagaatct
     7021 ccttgtcctt gccgtcgaga tcggcgagca cttccgcgcc gcccgaactt tctccctcag
     7081 gcgcaaccgg gcacacgttc actttcaact cgagcccggc aacacgcacc agattctcca
     7141 gaaattggcg cagcgcctcg gccgccgctt gccggtccaa cttcccatcg cggatgagtt
     7201 cttgagccat cgggcatttg agtgctgcga gccgcgttcg cctctctgaa atgtcttctt
     7261 aagacttctt cccgcgcacg ggctttgcgg gtgcggccac cggatgcgtc cggttcagat
     7321 accactgctg gacgatgccg accatgctgc tggtgagaat atacaccgcc aggccgctgg
     7381 aaatcggcga aatcatgaag atgcccgcca tggtgatggg catgattttc atcatcatct
     7441 gctgctgcgg gtctgtcgag gtcatcggcg tcatcttcga aacgagatac atggatgcgc
     7501 ccatcagcac tggcagaatg taatagggat cttttgctga aagatccaac acccacaacc
     7561 aagtagcctg gcgcatctcg atggagtaac gcagcgcggt gttcaatcca aaccacactg
     7621 gcatctgcag gaacatttgg aagcagccgc ccaccggatt gatgccctcg cggctgtaga
     7681 tcgccatgac ttccttgttc atctccgcct tgcgcggatc gcgcatggag tatttcttgt
     7741 atttgtcctg gatggctttg atttccggcg ccacgcgctg cattttcagc gtcgttttgt
     7801 aactcgaaat gcgcagcgga aagaggagca tattgatgac cagcgtgagg accacgatgg
     7861 cccatcccca attggggacg tagttgtgca gccacttcag cccgtgaaag agcggctcgg
     7921 cgatgaattc cagccatccg aaattgatca gcccgtgcag cggcgggctc attttcttca
     7981 agtcgtcata atctttcgga ccgacgtaga cacgcaaagt cagcggctgc gccgttgtcg
     8041 ccgcggccac ttggggcacc ggttccagcg cgtcttgtcc gtccaccttg acggtacgtg
     8101 acgccttcca gtagcgcgtc tccagtgcgt tcgatgtggc gccgctcgcg gggagaaatg
     8161 tcgccgcgaa gtatcgatcc tcaatgccaa cgaattcttt tccaccctgc cagaggcccg
     8221 ggccccactt ttccaggccg tccagctttt tgtgaaggaa tgtcgagact ttgccgcctt
     8281 cgctgtagaa cgtcgaaacc gtttctaccg gcgcagggtt cgtcaccgtc aaatcgccga
     8341 agccgccgag ccatccaagt cctgcggtga tgggactgcc gtttaacttg gcggaggttt
     8401 ctacgcgcac cacataagtg tgatcgaagc ggaaatgctt ggtgacttcc aggtggccgt
     8461 cgctccacgt gaattcaaca tcggccggtg cctgcagcgg ggtcgcgggg gaagagagct
     8521 gatagaggcc gtcgttcgcc gcattctgca actgttcatc atcgagcacc acggaaaagg
     8581 gccagccgcc ggtttgctgc gatgcatcag ggtgcacgac gtcgagcaca cgctgcggct
     8641 tcgcgtcatc cttgtaatgc ttcagctgcc agctcttcac caccgcgccg tggttcgaga
     8701 tttccacgcg gtaaaggctg ttttcgacga cgatcgtgcg ttcctgcgaa tcgttcttta
     8761 ccgttacgct cgtcgctgtc gcaggggcaa ttgttgccgg cacggcagtg gcatgcgatg
     8821 catttgccgc gggtgacgtc gccggacccg gtgtggccgg agcggtttgc gccgggcggt
     8881 tggcttgcgt tggctggacg ggcggcttcg gcccgaagaa ctttgcccag aggatgatca
     8941 ctccaaagga cagcagcgac gctacgagta ttcgcagctc cggcgtgaac ttgtcttttt
     9001 ggcccggcgc gctcatgagt gcgcctcagt cttcagaact ttcgccgcag ttgaactcgt
     9061 tggcatctct tcccatttct ccggaaccgg atcgtatcca aatttgcgcg agagtggatg
     9121 gcagcgcagt agccgcttca gctccagcca aacgccgcgc gtcacgccaa agcgttcaat
     9181 ggcctcgtag gcatagcgcg aacacgttgg ctcaaagcgg caacttcccg cgaacaaaat
     9241 cgacaaatac gctttgtaga aacgcagcgc gagcaacgcc gcgcgcacag caatggatcg
     9301 gtcttcgatt tgcgtggcgg cgacttgtgt agggctgact tgtgtagggg cggacttcag
     9361 tccgccctcc tcgccagcgt ccattgccgc aacagagagc gcaccagagt gcgcccctac
     9421 gcgctcttca gcagacgcaa caaatccgcg gtcaacgctg caaactctgc acgcgccacc
     9481 gaagtcttcg ggtgtatgac gatgtcccat cctgcgggta tctccagccg gtggcagcgc
     9541 acgacctcgc gcaggcgccg ccggatacga ttacgcacta ccgcgccgcc cagggccttc
     9601 ttgatgctga agccgaaacg gctctgcggc agctcgttgg cgcggaagaa aactgtgaag
     9661 tgggaactcg aacggcgctt tccggcgcgg tagacagcat caaactctcc gcgccgcacc
     9721 agccgcgctt cgcgcggaaa cagattccct tgcgcacccg aactggccgt gggcaacggg
     9781 ctccccgcat cactcggggg taagtcgatg gcgtcccttc ttgcggcgcg ccgccagcac
     9841 tttgcggcca cctttcgtct tcatgcgaac gcggaagccg tgcgtcttgg cgcgccggcg
     9901 tttgcgcggt tgaaatgtgc gctttggcaa aacaatcccc ctttgtagta cgcaatcgcg
     9961 gggcagtctc tttcacccgc aacggcagaa cagcccacta gcctcttcag cggctggttc
    10021 cgaatttagt agtgtattcg acccttgcgc acagggtcaa gatattgacg agggacagca
    10081 tttgtcagcg ggaattgccc gttttctcga agcgcgaaat gcaatgtcgg agatcatgga
    10141 atgcgtgact cattccagac aacaaaaaac cggggccgcg ggcgccccgg ttttcatccg
    10201 acattttatc gggatcggat ctgagtgtgt ggggagcgct tgcctcgcga gcaactgccc
    10261 gcctaggaat tggtctcgga ctccgcttcc ttgacgtccg cgtctgcctg cttcgttgcg
    10321 ttcttggctt cggcctttgc gttggcattc ggcgagagcg tctggatgga cttgcccaga
    10381 ctcatcttgc tcgatcctgt gccagttgga caggtcgata cttgcgcagg ggccgtgccg
    10441 gtcatgtccg cctgcaagca ggcaaacagg atcttcaaat tgtggctcac gtgaattgcc
    10501 gccatacact ggccgagatt acggaagcca gcgcaggcat ccttcaagtc tgttccggca
    10561 gggagcagtc ctttggattg gagcttcgag gtcagcttgg agtcaagctt ggtattacgg
    10621 tccaacacgg aagtgggtga gcttgccgaa ccctttgccg aactgcccat cgaagcgtgg
    10681 tcggacccat ggctcattgc cggtccatga ggcccgccgg cgcctcccgc tccggtcccc
    10741 tgcgagcgtt gggcgaaagc cggcacccct gcaaggtaca acgcacaaac agaaactgcc
    10801 gctaaccgaa ttatgcgatt catcatttct gcctcctgaa gtactagatc gaaaaaaact
    10861 tcctgcctaa acctgcggat ttcatctgct acccctatgg agcagaggcg tcagattaaa
    10921 agcgatgctg aatgagaccg tgtcgagctg aaagtgcacg ctgtggctgt agctgagatc
    10981 gaaatcgaca tatttcaaag ggctgaaatc gaggtcggca ttgtagccat tgtcgcgatc
    11041 gattgccgcg ccacccgtgg taagggcatt gttctggaaa actcggccat gtttcggcgt
    11101 tcccccttga ccgcccgctc ctttcgtcac gaggcggcta tacacctttt gtgttcccca
    11161 gggcgccacg tcatacgccg aagcgctgag cgtgagcgag tgcccgagat cgaatgaagt
    11221 gccgccctca aagtgcgcca cagggcccag tgtgaggaac ggccggtgcc agtagcgcgt
    11281 gtccaacaac gagttgccca cgcctgcgct ggcgaagggc gtccatcgcc cgaat
//
LOCUS       DASYVO010000356         3538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_123701, whole genome shotgun sequence.
ACCESSION   DASYVO010000356 DASYVO010000000
VERSION     DASYVO010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123701"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VGR03_12045"
     CDS             <1..984
                     /locus_tag="VGR03_12045"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1995053.1"
                     /translation="QQQLAKRDTQNVEAYHLYLRGRYFWNKRTAEGVKQGLAYFQQAI
                     EKDSSYALAYAGVADSYAVGNGGYLGLTGLEARPKARSAALKALELDDTLAEAHTTLA
                     DTYLYYEWDFTKADQEFRRAIAANPNYPTAHQWYAECLYSVGRYDEAIAEAKRAQELD
                     PLSPIIAGSLAATFLFARKYDDAIEQYKKTLQMDANLVNAHWDMGSAYAHKKMYPEAV
                     AEWQQGLILAGNPALAAILGEAYRGSGYQGFLQSWIDSRLKLQLVSERSYDLARLFAL
                     LGKNNETVHWLQKSLADHEGGIVWVKSDPIFDSLHSDPDFQTLVKKMNFPE"
     gene            complement(1066..3111)
                     /locus_tag="VGR03_12050"
     CDS             complement(1066..3111)
                     /locus_tag="VGR03_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEV1995054.1"
                     /translation="MGLQIDAIKEIEELKSEIATEGHTVGEPSNEGDSIGISLLDLAE
                     ILDQHKMWVESGGESGVKADLCGVNLAKADLTGVNLQGAHLHRMNLEGADLSMANLRG
                     ASLVRSNLQNANLLGTELRGANLMGANLYGAEGLWFGRLGGTNLFDAVLPESISAVDS
                     SKAIGDATKVARWFYFLTVGVSLICCLLIGFTSDVRLLLNASAFPIVRLGNLLPMTSL
                     YLGGPLVLLALSLRFHFLLLRLWGSMAALPAVFPDGQTLEKDGPWYLMGLVRRHFRWQ
                     GESRSAMTAFETVLSTVLAYWIVPATLFFYWLRYLPRQDFRGTLLHVFVITLSVATAT
                     SVPFVVSRVLRPGDIRLPKSKNIIRMILGTTRAALATGCILFLFSLGVNRGLPFDRGT
                     AADESPADVRRWASMVFQSVGFRPYADLTEATLSTPPPPGTDWTDETVEGIRGARLNQ
                     MNLRFARGYRAFLINAKLWRANLEGAYLSEADLRGANLREALLRSAILDRAQLTHAVL
                     VSANATGANFAAADMRFVDLSYGTFENAVLSNAKVSGASLYAVNLRNAQLLRTDLSRT
                     DMRDTKLEHAVLSFANLEQTDLSSAKLTEANLTGAILKGTILLDADLKKADLRGAYFT
                     GAILHGVEMDGANLAGADLRGASGITALQVCSALHGRAALMDPDLAVEVQQRCASSQ"
BASE COUNT          827 a         1101 c          922 g          688 t
ORIGIN      
        1 cagcagcaac tggcgaaacg cgatacccag aacgtcgaag cctatcatct ctaccttcgc
       61 ggaagatatt tctggaacaa gcgaaccgcc gaaggagtga aacaaggact tgcctatttt
      121 caacaagcca tcgaaaaaga ttcttcctac gctctcgctt acgccggagt ggcggactct
      181 tatgccgtgg gaaacggcgg atatcttggc ctgacgggac tcgaggctcg tccaaaggcc
      241 agatccgcgg cgctgaaggc gcttgaactc gatgatactc tcgccgaggc ccataccaca
      301 ctggccgata cctacctcta ctacgagtgg gacttcacca aagccgatca ggaattccgc
      361 cgcgccatcg ccgcgaaccc caattacccc accgcccacc aatggtatgc ggaatgtctt
      421 tacagcgtgg ggcgttatga cgaggccatc gcggaggcca agcgtgccca ggaactcgac
      481 ccgctctcac ccatcatcgc cggatcgctt gcggccactt ttcttttcgc gcggaagtat
      541 gacgacgcca tcgaacaata caaaaagacg ctgcaaatgg acgcaaacct cgtgaatgcc
      601 cactgggaca tgggttctgc ttatgcccac aagaagatgt acccggaagc ggtagccgag
      661 tggcaacagg gcttgatact tgcggggaac ccggctctgg cggctattct aggagaagcc
      721 tatcgaggtt ccggctacca gggtttcctg cagagttgga tcgatagccg tctcaaactt
      781 cagcttgttt ctgaacgttc ttacgatctc gccaggctct tcgctctctt ggggaagaac
      841 aacgaaacgg tgcactggct tcagaaatct ctcgcggatc acgagggagg gatagtgtgg
      901 gtcaagtccg atccaatatt cgattccttg cactccgacc cggattttca aaccctcgtt
      961 aagaaaatga atttcccgga atgagcattt atccacgagc aagagcgctc tgatcagacc
     1021 ccaccaaatt acagatccct cacagacctg acgttagaat acgacctatt gcgaggatgc
     1081 gcagcgctgt tgaacttcga ctgcaagatc gggatccatc aatgccgcgc gcccgtgcaa
     1141 agcggagcaa acctgcaacg ccgtgatccc cgaagcgccg cgcaaatccg cgcctgcgag
     1201 gtttgcacca tccatttcca ctccgtgcag aatcgccccc gtgaaatagg ccccgcgcaa
     1261 atccgccttc ttcaggtccg catcgagcag gattgtacct ttgagaatcg cgccggtcag
     1321 gttggcctcg gtaagtttgg cggaagatag gtccgtctgc tcgagatttg caaacgacag
     1381 taccgcatgc tcgagtttcg tatcgcgcat atccgtacgc gaaaggtcgg tccgcaacag
     1441 ttgagcattc cgcaaattga cggcgtaaag cgatgccccg gagactttcg cattcgaaag
     1501 caccgcattt tcaaacgttc cgtaagagag gtccacaaac cgcatatcgg ccgcggcgaa
     1561 atttgcgccg gtggcattgg cggaaaccaa taccgcatgc gtcaactggg cgcggtcgag
     1621 tatcgccgag cgcagcagcg cttcgcgcag attcgctccg cgcaggtcgg cttcggatag
     1681 atacgccccc tcaagattcg cgcgccacag ttttgcattg atcagaaaag ctcggtagcc
     1741 tcgcgcgaac cgcaaattca tttgatttag ccgcgcgccc ctgattcctt ccacggtctc
     1801 gtctgtccag tccgtgcccg gcggcggagg cgtcgacagc gtcgcttccg tcaagtccgc
     1861 gtacggccgg aagccaacgg attgaaagac catcgacgcc catcgcctta catccgccgg
     1921 cgattcatcc gccgctgtgc cgcggtcgaa gggcaatccc cggttcaccc cgagagaaaa
     1981 gagaaaaaga atgcatcccg tcgccagcgc cgcgcgcgta gtccccagga tcattcgaat
     2041 gatgtttttc gacttcggca gccggatgtc acccggccgc agcacccgcg acacgacaaa
     2101 gggtacactc gtcgccgttg ccaccgaaag cgttatgaca aacacatgca gaagcgttcc
     2161 ccggaaatcc tgccgcggca gataccgaag ccagtagaag aacagcgtcg cgggaacaat
     2221 ccagtacgcc agcaccgtcg aaagtaccgt ttcgaacgcc gtcatcgcgg atcgcgattc
     2281 cccctgccac cgaaagtgtc tgcgcaccag ccccatcaag taccacgggc cgtctttctc
     2341 cagcgtctgt ccatccggaa aaactgccgg caacgccgcc atgcttcccc acagcctcaa
     2401 cagaagaaaa tgaaatcgca gagaaagcgc cagaagaaca agcgggccac cgagataaag
     2461 actcgtcatg ggaagcagat tcccaaggcg tacgatgggg aacgccgacg cattgagcaa
     2521 caggcgcaca tcgctggtga agccaattag caggcagcag atcaggctca cgcccacggt
     2581 gaggaaataa aaccagcgcg cgacttttgt ggcgtcgccg atggctttcg aactgtccac
     2641 cgcggaaatc gattcgggaa gcaccgcatc gaagagattc gtcccgccca agcggccaaa
     2701 ccacaatcct tccgcaccat aaaggttggc gcccatcagg tttgcgccgc gcagttccgt
     2761 gcccagcagg ttcgcgttct gcagattcga acgcaccagg ctcgcgccgc gcaaattcgc
     2821 catcgaaaga tccgcgcctt caaggttcat tcggtgcaaa tgcgcaccct gcaaattcac
     2881 gcctgtcaag tccgctttgg ctaaattcac gccgcacaga tcggccttca cgcccgattc
     2941 tccgcccgat tccacccaca tcttgtgctg atccagaatc tcggcaagat cgaggagcga
     3001 gattccaatg gaatctcctt cgttcgaagg ttctcccaca gtgtgacctt cagtcgctat
     3061 ttcgctcttc agctcttcaa tttctttgat tgcgtctatt tgcaggccca ttcgaacgtt
     3121 ttcccccgac tctctctgcc cgctctcggg cattcttgct cgcagcaacc cctcaaactg
     3181 gctcgccatc gcagttctca acgcattcga attctgcgca ttcgaattct tcccagcacg
     3241 tttcgaattc cccccgccac cggccgcgtt tctgctgggc tgcctccatc ctctcctatc
     3301 agcactatgg aaatcgcctg cccggaagta caggcggcgc agaggtaacg ggcggacgga
     3361 atgccgccga tgcgcaagga ttggaagagg gattacaaag caagtgccga gcattcaaat
     3421 gcaaaggggg agagcgggaa aatacaagac tcgaatggca gcgaaaaaat gtgccggggt
     3481 agcttcgctg aggggccaag gcaccccggc ttatcctgcc cgcctccgca gtgagcga
//
LOCUS       DASYVO010000357         5022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_217344, whole genome shotgun sequence.
ACCESSION   DASYVO010000357 DASYVO010000000
VERSION     DASYVO010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217344"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VGR03_12055"
     CDS             complement(<1..262)
                     /locus_tag="VGR03_12055"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV1995055.1"
                     /translation="MPKKIHNPAILNPAAGTAEGITEVRRQEALLKTGALQNAILNSA
                     NFSSIATDEKGVIQIFNVGAERMLGYAAADVMNKITPADISDP"
     gene            complement(706..1497)
                     /locus_tag="VGR03_12060"
     CDS             complement(706..1497)
                     /locus_tag="VGR03_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV1995056.1"
                     /translation="MAEDNQVNQLVATRIFEKLGHQATVVSNGREAFVAFQAGKFDLI
                     AMDVQMPEMDGLDATSAIRAWEKTAGTHTPIIAMTAHAMKGDRERCLAAGMDGYTSKP
                     IRIGELEHAIAQLISPPNSAKVPVSEADQADGAIDHAALLAGVDGNRRVLRELVRLFL
                     ADCPRRLAEIKEAIRRGDAGALGRAAHTLKGSVGNFAAKNAFAAAQRLEIMGRDIDLD
                     NVGKACVRLESELALLSEELRKLTMNSSIRKTKTDKRGRQKGGQG"
     gene            1852..2742
                     /locus_tag="VGR03_12065"
     CDS             1852..2742
                     /locus_tag="VGR03_12065"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV1995057.1"
                     /translation="MSHTPVLAERILVADDDRTTRFAISSMLKKAGYAVTAAKDGAEA
                     LRNIQKKSFDLVFLDIWMPKLTGLEVLARVRAGESRPKIIIMTSYGTPETLLRAIREQ
                     AYEYLSKPFPPKEAVEVAARALKQDASPPIEVISAQPHWVELLIPCTREAAERIHSFL
                     MKLEADLPDDLRNTIGLAFKELLLNAVEWGGKLDPNRKVRIAQVRSSRMLLYRVADPG
                     PGFSFKGLTHAAVGQPADEPIAHTNVRDQLGIRPGGFGITMIRAIADELLYNEAQNEV
                     IFIKYLTASAGVSGTAEVPS"
     gene            3185..3415
                     /locus_tag="VGR03_12070"
     CDS             3185..3415
                     /locus_tag="VGR03_12070"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEV1995058.1"
                     /translation="MILRTVEQVRRVFLIIAGFTLLLVGGVMLATPGPGMLVIFLGLG
                     LLAAEFVWARRLMERIKHEGGRLVDRFAVTKK"
     gene            3471..4280
                     /locus_tag="VGR03_12075"
     CDS             3471..4280
                     /locus_tag="VGR03_12075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1995059.1"
                     /translation="MSHAMTRTAANWSGKWALITGASAGIGVALAEELASSGTKLVLT
                     ARRKDRLDELARRLTATYKISTEVFPADLADPNAPEKILAFTKEKGIEIDLLINNAGF
                     GQYGEFHSIEKERLLDMVQVNCHAVVHLTRLYLAEMVARKRGDVLILASTASFQAVPY
                     ISTYAATKAFDLLFAEGLAEEMKPYGIRVCALCPGSTESEFHVLSGQEKFRRRMETAE
                     KVAHTGLKALAAGKSYVISGLGNYLGAHGERLVPRRFVTKVAAGMFKPENK"
     gene            4504..>5022
                     /locus_tag="VGR03_12080"
     CDS             4504..>5022
                     /locus_tag="VGR03_12080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1995060.1"
                     /translation="MRIAEQERDDVLLRRAAKGDEEAFTHLYRLHQAAMYRFAVRMTG
                     NTWAAEEIVQDVFMTLMRNPKKYDPTRGALGAFLYGVARNRVMKHLERLPREVSLEEK
                     NEDGTGSGIVLQDASTPAMWMEKRERMQQVRAAVLELPAEFREAVVLCELEELSYEEA
                     AQMAGCPIGTIRS"
BASE COUNT         1061 a         1394 c         1493 g         1074 t
ORIGIN      
        1 gcggatcgga gatgtcggcc ggcgtgatct tgttcatcac gtcggcggcc gcgtagccca
       61 gcatgcgctc ggcgccgaca ttgaagatct gaatgacgcc cttctcgtcg gtggcgatac
      121 tcgagaaatt ggcgctgttg aggatcgcgt tctgcaaggc cccggtttta agcaaggcct
      181 cctggcgtcg gacctcggtg atgccctccg ccgtgccggc ggcggggttg agaatggcgg
      241 gattgtggat ttttttgggc atggcgacat ctatttaaga gcgaatcagt ctggcggatc
      301 gtgcggccga aggtgcgagc ggcacctcat aagaaagccg ctcgcctcgc ccagcttcca
      361 gctctcatca aggatagccg ccgccgcgcg caggcacaat gcgggattct aaggatgggc
      421 gtctctagtg gaggagacca caccgaccgg acggcgcgag ctcgaggaga tcgacgcgcg
      481 gctcaaggct ctcgagcttg cggagcgcca gcccgcgcca tagcagcaac ccgttccgca
      541 attaggctcc gctctagagg ccgggaatgg tcgcatctcc ggtttgccga ctgggacgga
      601 gcggctggtg aggcagatgc tgcgcgtggg gtgagtccag gaatccgaaa tctgacatca
      661 tgggttacag aaaaaggcgc tcttgcaaac tcataggcct tgctctcagc cctgtccacc
      721 tttttgtctc ccacgcttgt ccgtttttgt ctttcgtatt gaagagttca tggtcaattt
      781 tctgagctcc tcgctgagga gcgccagttc ggattcgagt ctcacgcatg ctttgccaac
      841 attatcaagg tctatatctc tgcccatgat ctccagccgc tgggcagccg caaaagcatt
      901 ctttgccgcg aagttgccga ctgaaccctt cagtgtgtgc gcagctctcc ccaacgctcc
      961 ggcatcaccg cggcggattg cctccttgat ttccgccaga cgccgaggac aatcggcgag
     1021 aaaaaggcga acaagttcgc gcagaactcg acgattgcca tcaacccctg ccaggagtgc
     1081 cgcatgatca atcgcgccgt ctgcttggtc cgcctccgac acaggcactt tcgccgaatt
     1141 tggtgggctg atcagttgag cgatcgcgtg ctcaagttct ccgatccgga tcggcttcga
     1201 ggtatagcca tccatgccag ccgctagaca acgctcgcga tctcccttca tggcatgcgc
     1261 tgtcatggct atgatggggg tgtgtgtgcc tgccgtcttc tcccaggcgc ggatggcact
     1321 ggttgcgtca agcccatcca tctccggcat ctgcacgtcc atggcgatga gatcgaactt
     1381 accggcctgg aaggcgacaa acgcctcgcg gccattgctg acaacagtag cctggtggcc
     1441 cagcttttca aagatccgcg tcgctaccag ctggttgact tgattgtctt ccgccactag
     1501 cacttgcagc ctgcgctgac taggctgcga tctcttacca ggcagggttg acatgtttga
     1561 agtagccatc agtgccgacg atacaaaaga ggtttaagga caggtcgata aactcgcgca
     1621 ggtcgtcttc tgttggcgaa ttgaaacgtg ctggtgaact caccgttccc ccgatcggaa
     1681 cactcaaaaa ccggaccagg gcactcattt tggtacaaag ccggaaattg cgcaacttgg
     1741 cgggttcttg cgggtcactg cgatcgaccg cggttgactc gtgtttcttg cggaggtagc
     1801 atttggtgcg aaatgcgggt tcgttggatc cagccgcatg aggtggcatc gatgagccac
     1861 accccggtct tagcagagag aatccttgtc gccgatgatg accgcacaac tcgctttgcc
     1921 atctccagta tgctgaagaa agcgggctat gcggttactg ctgcgaagga tggcgcagaa
     1981 gcgttgcgca acatccagaa aaaaagtttt gatctggtat tcctggacat ttggatgccg
     2041 aagttgactg gccttgaggt acttgcccgg gttcgcgcag gcgaatctcg cccgaaaatc
     2101 atcatcatga cctcctacgg cactccggag accctgctgc gcgctatccg ggagcaggcc
     2161 tatgaatacc tgagtaagcc ttttccaccg aaagaggcgg ttgaagtggc cgcgcgagca
     2221 ctcaaacaag acgcttctcc gccgatcgaa gtcatttctg cccagcccca ttgggtggag
     2281 ttgcttattc cgtgcacgcg ggaggctgcc gaacgcattc attcctttct aatgaagctg
     2341 gaagccgatc ttccggatga cctgcgcaac acgatcggac tggcgttcaa ggagctgcta
     2401 ctcaacgccg tggagtgggg cggaaagctg gatccgaacc gcaaagtgcg gattgctcaa
     2461 gtgcgctcct cgcgcatgct gctgtatcgc gtggcagacc cgggcccagg ctttagcttc
     2521 aaaggcttga cccacgctgc cgttggtcag cctgcggacg aaccgattgc acatacgaac
     2581 gtccgcgatc aactcggcat acgtccaggc ggattcggca ttaccatgat tcgcgcgata
     2641 gccgatgagc tgctctacaa cgaggcgcag aacgaggtga tctttattaa atacctgacc
     2701 gcatctgccg gcgtttctgg aacagcggaa gttccctcgt gacatcggca ctgggcagat
     2761 acggcggcat cagacgcgga aactggacaa gtgcctcctg gctccccaga agttgtggga
     2821 agtgcgagat gcgtcggtct cccggcgagt aaatccctat aaacgcggca gtgtttagtg
     2881 acacctcgct gctatgcccc ggggaagtcg gcttcctgtc acttgcccta acgtcgctgc
     2941 ctgaaaagcg accctgagtg tggtcactgc ttccggcgtt tggtttggtg tctttggtgt
     3001 ctgtttgggg cggaaattgg tagtatgtga ttggaacagt tgaaggcatt gaaactgtgg
     3061 aaaccgctga atcgcctgta ttcatgcggt ttgtcgtgga tgtcgatgaa aagacagaac
     3121 tacgcggaaa gggcctgggg ataatcccac cctctccgcc agaaagccgt ttccctaagg
     3181 acccatgatt ctccgcactg tcgagcaggt tcgccgggtc ttcctcatca tcgctggatt
     3241 caccttgctc ttggttggtg gcgtaatgct ggccactccc gggccgggaa tgttggtgat
     3301 ttttctcggg ctcggcttgc tggccgctga atttgtttgg gcgcgacgcc tgatggagcg
     3361 aatcaagcac gaaggcggac gcttggttga ccgctttgcg gtcaccaaga aatgaagacg
     3421 ccgtccgccg ttagggaaag tagcagaaaa cgtcttttgt ggagagcacc ttgtcacatg
     3481 cgatgacgcg gacggcggcg aattggtctg ggaaatgggc gctgatcacc ggagccagcg
     3541 ccgggattgg cgtggcactc gcggaagaat tggcatccag tggaacaaag ctggtgctga
     3601 cggcgcggcg aaaagatcgc ctcgatgaat tggcgcgcag gctgaccgca acctacaaga
     3661 tcagcaccga agtgttcccc gcggaccttg cggatccaaa cgccccggaa aagattttgg
     3721 ctttcacgaa agaaaaagga attgaaatcg atctgctgat caacaacgcc ggtttcggcc
     3781 aatacggcga atttcactcg attgaaaaag aacggttgct ggacatggtg caggtgaatt
     3841 gccacgcggt ggtgcacctt actcggctgt atttggcgga aatggtcgcg cgcaagcgcg
     3901 gcgatgtgct cattctcgca tcaaccgcat ctttccaggc cgttccctat atttcgacct
     3961 acgccgccac aaaagcgttc gatctgcttt tcgcggaggg actcgccgag gagatgaaac
     4021 cctatggaat ccgcgtgtgc gccctttgcc caggctcgac ggagtccgaa ttccacgtcc
     4081 tttctggcca ggaaaaattc aggcgccgca tggaaactgc ggaaaaagtc gcgcacacgg
     4141 gactgaaagc tctggctgcc ggcaagagct acgtcatctc cggcctggga aattatctcg
     4201 gcgctcacgg cgaacgcctc gtcccccgcc gcttcgtcac taaagtcgcc gccggcatgt
     4261 tcaagcccga aaacaaataa agagcccttc ccgccggcgc actaacgatc gcgagtggat
     4321 gtttcagctc acccatactc gcaaacgctt cctcgacacg cggaggcccc cactcgcgtc
     4381 taagctattg tgaaaacaag tactttctgg ccttcagtcc ggatgacttc ttcggacggg
     4441 aactttttta cgccgcggcg ggaaatgata gatgagagcg gacagagcgt ctaaccagga
     4501 gagatgagga tcgccgagca agagagagac gacgttctgt tgcggcgggc cgccaagggc
     4561 gatgaggagg ctttcacgca cctctatcgg ctgcaccagg cggctatgta ccgctttgcg
     4621 gtacgcatga cgggaaacac ctgggccgcg gaagagattg tgcaggacgt gtttatgacc
     4681 ctgatgcgaa atccgaaaaa gtatgacccc acgcgcggag cgctcggagc attcctctac
     4741 ggagtggcgc gcaaccgcgt gatgaaacat ctcgaacgat tgccgcggga agtttcgctt
     4801 gaggagaaaa acgaagacgg cacgggctcc ggaattgttt tgcaggacgc gtccacgccg
     4861 gcgatgtgga tggaaaaacg tgaacgcatg cagcaagtga gggcggccgt gctggaattg
     4921 cccgcggagt ttcgcgaagc tgtggtgtta tgcgagctgg aagaactgag ttatgaggaa
     4981 gccgcgcaaa tggccggatg cccgattggg acgattcgtt cg
//
LOCUS       DASYVO010000358         3711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_190127, whole genome shotgun sequence.
ACCESSION   DASYVO010000358 DASYVO010000000
VERSION     DASYVO010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190127"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VGR03_12085"
     CDS             complement(<1..564)
                     /locus_tag="VGR03_12085"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV1995061.1"
                     /translation="MATQTQQVRRMNLTLAETDPEVAEAIRDEARRQATGLELIASEN
                     FVSEAVLEAMGSVFTNKYAEGYPGKRYYGGCEHADKVESLAISRAKQLFSAEHANVQP
                     HSGTQANVAVYMAALQPGDTVLGMNLAHGGHLTHGHPLNFSGRMYKFIPYGVRQDNEL
                     LDYEEVERLAAEHKPKMIVAGASAYSRI"
     gene            complement(691..1158)
                     /gene="rpiB"
                     /locus_tag="VGR03_12090"
     CDS             complement(691..1158)
                     /gene="rpiB"
                     /locus_tag="VGR03_12090"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEV1995062.1"
                     /translation="MPESTRKPRIALGADHAGFHIKEVIKGFLEGQGYPVDDAGTWSE
                     ESVDYPDYARKVADEVAQGQAPLGILVCGTGMGMAIAANKVAGIRAATAHDAITARAA
                     REHNDANVLTLGGRVVDPERAVEIVREFLDASFAGGRHQRRIDKITEIDRARK"
     gene            complement(1341..1664)
                     /locus_tag="VGR03_12095"
     CDS             complement(1341..1664)
                     /locus_tag="VGR03_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1995063.1"
                     /translation="MSKRNIFSELKEGIAAMKSHREGKLTLRSYRVVPGTLPKVNSKL
                     IRDTRKKLHCSRAVFARKLRINLRTLEKWEQGRAKPNPQAAALVLLVRRYPDTLQRLE
                     KLTAG"
     gene            complement(1664..2038)
                     /locus_tag="VGR03_12100"
     CDS             complement(1664..2038)
                     /locus_tag="VGR03_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995064.1"
                     /translation="MEFIEAPAFTRLISDYLADDNYGELQNQLAANPNLGDLMPGTGG
                     FRKLRWRDPRRGKGRRGGLRIIYYHFPADQQIWLMTLYDKDEASDLTSREKKAIKSTL
                     EAELQARHAKPLARKMRSRRTR"
     gene            complement(2108..2860)
                     /locus_tag="VGR03_12105"
     CDS             complement(2108..2860)
                     /locus_tag="VGR03_12105"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV1995065.1"
                     /translation="MAMRMRNHMVPAILAILTTSLPAAAQDKPAFVEIRQSEASFKSE
                     GTSISAELFQPEAAGKYPAVIVLHGEDGMAFGRYPYVHIAASVARAGNVALIVRYFDR
                     TDTPLATYWTQAQKIAAWRKTVNDAVTYAATLADVDANRIGLVGVSLGASLALALAAQ
                     DARVHAVVEYYGEPPDDFAAHWRKMPPVLILHGEKDSILKVDAAYRLEALLKKNQLPY
                     EIKIYPGQGHGFQGADLADALARTIAFFRQHL"
     gene            complement(2905..>3711)
                     /locus_tag="VGR03_12110"
     CDS             complement(2905..>3711)
                     /locus_tag="VGR03_12110"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1995066.1"
                     /translation="SHAYRMLIFSVDAAPQTVVPKARAAALKALELDPALDEAHTSIA
                     VVLGQYDWDWLGAEREHKRAIELNPNNSPAHLFYSEMLSILGRHSEAVSEAQRARALD
                     PVSMPALLMVGRALFYGRQYDRAVQEFTQSIEVSAGFWPMHLFLGKVYGEQRLYEKAL
                     AELRKAQGPTPHATASMGYVLGKSGKKVEAKQILGELMLRANQTYVSPTHIANNYVGL
                     GEKDQAFAWLEKGFEARDSHMEFLGVDPMYDSLRSDPRFVDLLGRLHLPH"
BASE COUNT          665 a         1124 c         1097 g          825 t
ORIGIN      
        1 gatccgcgag tacgcgctcg cgcccgccac gatcattttc ggtttgtgct ccgcggcgag
       61 acgctccacc tcctcatagt cgagcagctc gttgtcctgc cgcacgccgt agggaataaa
      121 cttgtacatg cgaccagaaa aattcaaggg gtggccgtgc gtcaggtgac cgccgtgcgc
      181 gaggttcatg cccagcacgg tgtcgcctgg ctgcaacgcg gccatataca ccgcgacgtt
      241 ggcctgcgtg ccggaatgcg gctgcacgtt ggcgtgctcc gcgctgaaca gttgcttcgc
      301 gcgcgagatg gccagggact cgaccttgtc ggcgtgttcg cagccgccgt aatacctctt
      361 gccgggataa ccctccgcgt acttgttggt gaataccgag cccattgctt cgagcaccgc
      421 ttcggagacg aaattctccg aagcgatcag ctccaggccg gtggcctggc ggcgcgcctc
      481 gtcgcgaatg gcctcggcga cttccgggtc ggtctccgcg agcgtcaagt tcattcgccg
      541 cacttgctgc gtttgcgttg ccatggtgtc tcccgtgcca agccgcgcgg tcattctgag
      601 cgcagcgaag aatccctctt cagttttctc gatggcggag aatgagagat tcttcgctgc
      661 actcagaatg gcaaagaatt ttcgcgtgcg ctactttctc gcgcgatcga tctccgtgat
      721 cttgtcgatg cgccgctggt gccgcccgcc ggcaaatgac gcgtcgagga attcccgcac
      781 gatctcgacc gcgcgctcag gatccaccac gcgccctccc agagtcagga cgttggcgtc
      841 attgtgctcg cgggccgctc gcgcggtgat ggcgtcgtgg gcggtggcgg cgcggatgcc
      901 tgcgactttg ttcgccgcaa tggccatgcc catccccgtg ccgcacacca ggattccgag
      961 cggcgcctgg ccttgggcca cctcatcggc caccttccgc gcgtagtcgg gataatccac
     1021 cgactcctcg gaccatgttc cggcgtcatc gaccggataa ccctgcccct ccagaaaacc
     1081 tttgatgact tccttaatat gaaacccggc gtgatcagcg cccagggcaa tccgcggttt
     1141 tcttgtgctt tccggcattt ttctccgcaa ctcgattcta ggggagccct acctgatttg
     1201 caatccgcgc tacaggtgcc ggtggagaaa ggccactgcg cgcgctaacg cgtcggcgtt
     1261 gtcggcgccc tggaaggctg tgttgctccg tcttcggtaa ccgctcaaac gcctcggttt
     1321 tttaattcat ccgagtgcga tcatcctgct gtcagctttt ccagccgttg cagggtgtcc
     1381 gggtagcgcc ggacaagcag gaccaaggcg gcggcttgag ggttgggttt ggcgcggccc
     1441 tgctcccact tttcaagggt ccttaaattg atccgcaact tgcgtgcaaa gacggcccgt
     1501 gagcaatgca gtttttttcg cgtgtcccga atcagttttg agttcacctt cggaagcgtt
     1561 cccgggacta ccctgtagct gcgcaaggtg agcttgcctt cgcggtggct cttcatcgca
     1621 gcgatgcctt ccttcagctc gctgaaaata tttctcttgc tcattatcgt gtcctccgcg
     1681 atctcatttt tcttgcaagc ggcttcgcgt gacgtgcttg cagttcggct tcgagggtgc
     1741 tcttgatggc cttcttctct cttgacgtga ggtccgaagc ctcgtccttg tcgtaaagcg
     1801 tcatcagcca tatctgttgg tctgccggaa agtggtagta aatgatccgc agaccgcccc
     1861 tccggccttt accgcgtctt ggatccctcc agcggagctt tcggaaccct cccgttcctg
     1921 gcatcaagtc gcccaggttc ggattggccg ccagctggtt ttggagttcc ccgtagttat
     1981 catcggccag ataatcggag atgagccggg taaaggccgg agcttcaatg aattccattt
     2041 ggtcgaaggt acgctcttag cgtacaaatg acattgtacg ctattggcgt agagttgtca
     2101 accgccgcta gagatgctgg cggaaaaagg caattgtgcg cgcgagggca tcggcaagat
     2161 cggcgccctg gaagccatgg ccctggccgg gatagatttt gatctcgtag ggaagctggt
     2221 ttttcttgag cagagcctcg agccggtagg cagcgtccac tttcaggatg gaatccttct
     2281 caccatgcag gatcagcacg ggcggcatct tcctccagtg cgccgcaaag tcatccggcg
     2341 gctcgccgta gtattccacc acggcgtgca cgcgcgcgtc ctgagcggcc aacgccaaag
     2401 ccagcgaggc gccaagcgaa acgccgacca gaccgattcg attggcgtcc acgtcggcca
     2461 gcgtcgcggc gtacgtcacc gcatcgttta ccgtcttgcg ccaggcggcg atcttctgtg
     2521 cctgggtcca gtaggtggcg agaggcgtat ccgtgcggtc aaaatagcgg acgatcagag
     2581 cgacgtttcc agcgcgggcc acgctcgcgg cgatatgaac gtacggatac cggccaaacg
     2641 ccatcccgtc ttcgccgtga aggacaatta ccgccggata cttgcccgcg gcttcgggct
     2701 gaaacagttc cgcggagatg gacgtgccct cgctcttaaa gctggcctcc gactgtctga
     2761 tctccacaaa ggcgggcttg tcctgcgccg ccgccggcag actcgtcgtc aggatcgcga
     2821 gaatggccgg aaccatgtgg ttgcgcattc gcatcgccat gagtgtacgg cttcccgcta
     2881 tggagttcga ggggcacgca gcgattaatg gggtaagtga aggcgaccaa gcagatcgac
     2941 aaagcgcggg tcggaacgca gcgagtcgta catcgggtcc acgccgagga actccatgtg
     3001 ggaatctctt gcctcgaaac ccttctccag ccacgcaaaa gcctgatctt tttctcccaa
     3061 accgacgtag ttgttggcga tgtgtgttgg ggaaacgtag gtctggttcg cgcgcaacat
     3121 cagttcgccc agaatttgct tcgcttccac tttcttgccg gattttccca ggacgtagcc
     3181 catcgacgcc gtcgcgtggg gagtgggtcc ctgcgctttg cgcagctcgg cgagcgcctt
     3241 ctcatacaag cgttgttctc catatacttt tccaaggaat aggtgcatcg gccaaaaccc
     3301 cgcgctgact tctatggatt gcgtaaattc ctgcaccgcc cggtcgtact gccgtccgta
     3361 aaacaatgcc cgtcccacca tgagcaaggc cggcatagag acgggatcga gcgcccgggc
     3421 gcgttgcgct tcagaaacag cttcggaatg ccggccgagg atggacagca tttctgaata
     3481 gaatagatgt gcgggagagt tgttagggtt cagctcgatg gcgcgcttgt gttcgcgctc
     3541 ggccccgagc caatcccagt cgtattgtcc cagaaccacg gctattgagg tgtgagcctc
     3601 atctagagcg gggtcaagtt ccagggcctt gagcgctgcc gccctggctt tcgggacaac
     3661 cgtctgggga gccgcgtcga cactgaaaat cagcatgcgg taagcatggg a
//
LOCUS       DASYVO010000359         4138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_6249, whole genome shotgun sequence.
ACCESSION   DASYVO010000359 DASYVO010000000
VERSION     DASYVO010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6249"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..1562
                     /locus_tag="VGR03_12115"
     CDS             288..1562
                     /locus_tag="VGR03_12115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV1995067.1"
                     /translation="MRNRILLYLVLAGVMAYVGVELSGRKPVPKVSAVTPMRENLVSS
                     ISSNGKVEPISPYVMRAQLDTFVEKVRATEGQTVKKGQVILELDVKDAAAKLSEAKGK
                     LLRAQDDLRAAKAGGRTDDAARVAGDLAKMQAERDRLQKNHDVLQRLIAQQAATQDEL
                     AANDLALTKAQAEVTRLTAAKLEFERSVKLDTDQAALHVQQIQNEAASLDEKVRQGRI
                     TAPADGTLYSLPVKAGDYVKLGDLLAEMADLHKVRVRAFIDEPELGALEANEPVKITW
                     DALPSKTWVGKTEIIPKQVVPHGARSVGELLCAVNNDKLELLPNINVNVRINSRERIG
                     VLSVPRGAVAAEGGRRYVFVVRKNQLGVGKSTLEKREIHVGIADATNYEVVSGLQEGD
                     LVALPGEVDLRDGMTVIVITTEVSNFPERWNG"
     gene            1555..2547
                     /locus_tag="VGR03_12120"
     CDS             1555..2547
                     /locus_tag="VGR03_12120"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1995068.1"
                     /translation="MVRRIEWLLAALCIASTAIAAANLKQDAGLEAGRRAYEASDYPK
                     AIQALLAAAVKEPQNGDVQLLLAKSYLELQEHDPAIKSAEKAVAIDPRNSIYHEWLGR
                     AYGEKADHASWFSAISLAKKTRKEFETAVQLDGKNFSARQVLIEFDCSAPGLVGGGEE
                     KALSQIKQLAELDAAEGHYAAGNCRRQKKDFAVADEEFAKALENNPKSPELIYDIGDY
                     GVKRSQPERLLAVADMGEHLAPGDPRGKFFRGVALVLKRENPEEAERLLREYAKKAPM
                     RSGYPRAATAHAWLGRLFDEQNKVEEAVKEFESALKLDPKNRMAQEALKRLKKG"
     gene            2590..3786
                     /locus_tag="VGR03_12125"
     CDS             2590..3786
                     /locus_tag="VGR03_12125"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1995069.1"
                     /translation="MHIRETVSVAIDALRANKLRAILTSLGVIIGSASIVLVVTVALT
                     SKTFVLSQIESIGSNLVQVELVQRPDKPQPLSYEMTLEDMEAAKSIPNVIEVAGTREL
                     PMTVVAGGVERPVNLVGVTEGYQAIRRLLVLRGRYLDAGDMEMRSKVCLITTQLADRI
                     FGQENPIGRPIRMGELTFTVIGVFRERVETFGLSDIQENSVILPFTLMKYYTGMEVVR
                     LLDVQAARAEDVPSVERQVGQLLRARHPAEAEYKVLTLTAILNAARNISLALTIVLII
                     IAFIALVISGIGIMNIMLVTVKERTREIGIRKAIGAARREIMYQFLIESFVISGGGAV
                     IGILIGIAIPVGAQFFLPGNLRVPVSGLSVLIAFVVSCSTGLFFGYLPANQAARLQPV
                     ESLRYE"
BASE COUNT          965 a          960 c         1464 g          749 t
ORIGIN      
        1 cataggcagt ctacggcatc ccgaggatac aaaagacgca tccgtcagag gtgagaaagg
       61 ctcgatccgg agaatcggtt acgagagagc agggcgcagg gtaaacggcg cctgcaagga
      121 atctagagaa agccgcgctg ctcttgctag aatgagatgc cttgacgcct acttgacagg
      181 ggtcaagcaa gccacggaca agtaagagaa acgctgcgcc tgagggatgg gtcctcgcga
      241 cttgcggagt gggggaccgc gcagaatagg aaactatgga tcgcaggttg cgaaaccgaa
      301 ttttgctcta cctggttctg gcgggagtga tggcgtacgt gggggtggag ctgagcgggc
      361 gaaagccggt gccgaaagtt tcggcggtga cgccgatgcg cgaaaacctg gtctcgtcca
      421 tcagcagcaa tggaaaggta gagcccatct cgccgtacgt gatgcgagcg caactggata
      481 cgtttgtgga aaaggtgcgc gcgacggagg gacagacggt caagaagggg caagtgattc
      541 tggaattgga tgtaaaggat gcggcagcga aactctccga ggcgaagggt aagctcctgc
      601 gggcgcagga cgatttgcgg gccgccaagg cgggcggcag aacggacgat gcggcgagag
      661 ttgcgggcga cctggcaaag atgcaagccg agcgcgaccg gctgcagaag aatcatgatg
      721 tgctgcagcg gttgatcgcg cagcaagccg cgacgcagga cgagctcgcg gcgaacgacc
      781 tggccttgac gaaagcgcaa gcggaagtga cacggctgac ggcggcgaaa ctggagtttg
      841 aacggagcgt gaaactggac acggaccagg ccgcgctgca cgtgcagcag attcaaaacg
      901 aagcagcctc gctggatgaa aaggtgcggc aaggacgaat caccgcgccg gcggatggaa
      961 cgctctactc cttgccggtg aaagccggag attacgtgaa gcttggggat ctgctggcgg
     1021 aaatggcgga cctgcacaag gttcgtgtgc gggcgttcat cgatgagccg gagctagggg
     1081 cgctggaagc gaatgagccg gtgaagatta cctgggacgc gttgccaagc aagacgtggg
     1141 tgggaaaaac ggagatcatt ccgaagcagg tggtgccgca cggagcgcga agtgtgggtg
     1201 agttactttg cgcggtgaac aacgacaagc tggagctgct gccgaatatc aacgtgaacg
     1261 tgcgtatcaa ttcgcgggaa cgcatcggtg ttttatccgt gccgcggggc gcggtggccg
     1321 ccgagggcgg taggcgatat gtatttgtgg tgagaaagaa tcagctgggc gtgggcaaat
     1381 ccacgctgga aaagcgggag attcacgtgg gcatcgcgga cgccacgaac tacgaagtgg
     1441 tgagcggcct tcaagaggga gatctcgtgg cccttccagg ggaagtcgat ttgcgggatg
     1501 ggatgaccgt cattgtcatt acgaccgaag tttccaattt cccggaacga tggaatggtt
     1561 aggcggatag agtggctgct tgcagccctg tgcatcgcga gcaccgcgat tgccgcggca
     1621 aatctcaagc aagatgcggg tctcgaagcg ggacggcggg cttacgaggc cagtgactat
     1681 ccgaaggcca tccaagcgct gctggcggct gcagtcaagg aaccgcagaa tggcgacgtg
     1741 caattgctgc tggcgaaaag ttatctggag ctgcaggagc acgaccccgc gatcaagagc
     1801 gcggagaagg cggtggcgat cgacccgcgg aattcgatct accatgagtg gttagggaga
     1861 gcgtacggag aaaaggccga tcatgcgagc tggttttcgg caatatcgct cgcgaagaag
     1921 acgcgcaagg aattcgagac ggcggtacaa ctcgacggca agaatttttc agcacggcag
     1981 gtgctgatcg aatttgattg cagcgctccg ggcctggtgg ggggtggcga ggagaaagcg
     2041 ttgtcgcaga tcaagcaatt ggcggagttg gacgccgccg aagggcatta cgcggcggga
     2101 aattgccggc ggcagaagaa agattttgcc gtggcggacg aggaatttgc gaaagcgctg
     2161 gagaacaatc ctaaatcgcc ggaactgatt tacgacatcg gggattatgg cgtgaaacgc
     2221 agccagccgg agcggctgct ggcggtagca gacatggggg agcacctggc gcccggcgac
     2281 ccgaggggaa agtttttccg gggcgttgcg ctggtcttga aaagggaaaa ccctgaggaa
     2341 gccgaaagat tgctaaggga atatgcgaag aaagcgccga tgcgatcggg atatccgcga
     2401 gcggccacgg cacacgcgtg gctggggcgt ctcttcgacg agcagaacaa ggtggaggag
     2461 gcagtgaagg aatttgaaag tgcgctgaaa ctggatccaa aaaatagaat ggcgcaggaa
     2521 gcgttgaagc ggctgaagaa gggctaagag tttcggccgc ctctggagaa ttccggtcaa
     2581 aggtcaacga tgcacattcg tgagacggta tcggtggcga tcgacgcgct gcgcgcgaat
     2641 aaattgcgcg ccatcctgac gtcgctggga gtgattatcg ggagcgcttc gatcgttctg
     2701 gtcgtcaccg tggccctcac cagcaagaca ttcgtgctct cacaaattga aagtatcggc
     2761 tccaacctgg tccaggtgga attggttcaa aggcctgaca agccgcagcc gcttagttat
     2821 gaaatgacgc tggaggacat ggaggccgcc aaatccattc ccaatgtgat cgaagtggcg
     2881 ggaacgcgcg aactgcccat gaccgtggtt gccggcggag tggagcgccc cgtgaatttg
     2941 gttggagtga cggaaggata ccaagcgatt cggcggctgc tcgttttgcg cgggcgctat
     3001 ctcgatgccg gagatatgga gatgcgaagc aaagtctgct tgatcacgac gcaactcgcg
     3061 gatcggattt ttgggcaaga gaatccgatc gggagaccga ttcgcatggg agagttgacg
     3121 tttacggtga tcggcgtgtt ccgcgagcgc gtcgagacgt tcggcctctc ggatattcag
     3181 gaaaactcgg tcatcctccc cttcacgctc atgaagtatt acaccggaat ggaagtggtg
     3241 cgtctgctgg acgtgcaagc agcgcgggcg gaagacgtgc catccgtcga gcggcaggtg
     3301 ggccaactgc tgcgggcccg gcatccggcg gaagcggaat acaaggtgct aacgttgaca
     3361 gcaattctga acgcggcgag gaatatctcg ctggcgttga ctatcgtgct catcatcatc
     3421 gcgttcatcg cgctggtgat cagcggaatc gggatcatga acatcatgct ggtgacggtg
     3481 aaagagcgga cgcgggagat cgggatccgc aaagcaattg gagcggcgcg gcgcgagatc
     3541 atgtatcagt tcctgatcga atcgttcgtg atcagcggag ggggagcagt catcgggatt
     3601 ttgatcggca tcgccatacc ggtaggcgcg cagttttttc tgccgggaaa tctgcgggtt
     3661 ccggtctcgg ggctcagcgt gttgatcgcg tttgtggtgt cgtgttccac ggggcttttc
     3721 ttcgggtatc tgccggcgaa ccaggcagcg agactgcaac cggtcgagtc gttgaggtac
     3781 gagtgaaggc aggaaatagg ggatagggaa tagggaatag agaatagaga gggaaagtga
     3841 atcagggggc ggcgattggc ggcccttttt atttttcaga gaactgagtc ttgggccgga
     3901 ggccagagat tcgtggagcg agactccgca agatattgtt gaggcgagcc tttggaaatt
     3961 gagcggcgac gaaggagcat gaaaatgcaa ttactgggac gtgttggggg gatgactgcg
     4021 ctgctggtgt tgtgcggggg cgctgcgggt gcgcaagagc cgtccgcgcc gaaaacgagt
     4081 cagcctgcga ctcaagaagg gtggaaggat ggggctcccg tggcgctgac gctaaagc
//
LOCUS       DASYVO010000360         4356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_38511, whole genome shotgun sequence.
ACCESSION   DASYVO010000360 DASYVO010000000
VERSION     DASYVO010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38511"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VGR03_12130"
     CDS             <1..666
                     /locus_tag="VGR03_12130"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF012677.4"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV1995070.1"
                     /translation="AAGMHPSPVPHADIVSTTTHKTLRGPRGGLVLCKAAFAKELDKL
                     TFPGTQGGPLVNTIAAKAVCLKEAMEPSFKEYQKQVVANTKAMAASVAKHGFRIVTGG
                     TDNHLFLVEVHPRGITGTDAEKALDRAGITVNKNSIPFDPLPPMKGGGIRLGSPSVTT
                     RGMREPEMEQIGAWIAEILSNIGNAEVEQRVRKQVAELAGRFPIYEARLRGGPARIEH
                     AHV"
     gene            693..1379
                     /locus_tag="VGR03_12135"
     CDS             693..1379
                     /locus_tag="VGR03_12135"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HEV1995071.1"
                     /translation="MNIFKSTSDFEHWLAGQLPIIRQDLALKHQHMAEAAFPFFRATF
                     YRWLQLWPEVCAHLAKAPAVLGVGDLHIENFGTWRDEEERLIWGVNDLDEAWPAPYAL
                     DLVRLITSVYVAIEEEHLCLAKRVAAEAIEEGYRDALAAGGKPFILAEDHRWLRLLAL
                     GKLRDPVQFWDKLEHLPPYTAKPPEEARKLLEKSFPIALEEYLLKKRIAGLGSLGHPG
                     YSPCPDGEER"
     gene            1466..1786
                     /locus_tag="VGR03_12140"
     CDS             1466..1786
                     /locus_tag="VGR03_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995072.1"
                     /translation="MQRSIRVPDPCVRFHGRWVVRRLAPDCSRIELASLSKDRDEARL
                     LYSMGWETANMHFATPRAMAKVEHDLAARRGRWLHKAAKAMLAVTAKDWKKWQRDWKR
                     SAPR"
     gene            2118..2582
                     /locus_tag="VGR03_12145"
     CDS             2118..2582
                     /locus_tag="VGR03_12145"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1995073.1"
                     /translation="MMDTEAFREFKTGLTLLRDNYAIKALPHMRRAVDLDKNNPYYMS
                     YLGVVLARSEGKWGEAERLCDSAVRMKRNQAQLYLNLAEVYATAGRRDDAVDALETGL
                     KYARRDIRLTIAMNKLTARRPPVLSFLGRRHPLNRQLGILRHRTMRIFGQPS"
     gene            complement(2687..3097)
                     /locus_tag="VGR03_12150"
     CDS             complement(2687..3097)
                     /locus_tag="VGR03_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEV1995074.1"
                     /translation="MATQQDLMTKKLSTGSSPKSAISNTERRGSRRCKITQLMRIRPS
                     DPNKDHFEDMRGSVSVSRSGVFFQSSESGYEIGMRLFVTMPYSQDPASMAREYLAEVV
                     RRDVLPTGMFGIGFKILMEMGMQHSYNFGAGNSR"
     gene            3552..4340
                     /locus_tag="VGR03_12155"
     CDS             3552..4340
                     /locus_tag="VGR03_12155"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1995075.1"
                     /translation="MSTATRQILQVGEQAQKRINSNGDRRTNIAVGESAARVSGGAVV
                     ERKPRVFVAAENRLLREALSRMLVKSGEVEVIGTDMAEPFRTEDLLKEETDILLLSSR
                     GNRHEDLTAVRRVRTTAPKVQILLIGVTGEEAEFLQCVRAGVRGYLPRDASAEDVVEA
                     VRALQGGEATCPGTLCATLFRYFEREATSFPSASAHQRLGLTRREQQLIPLIAEGLTN
                     KEIANRFCLSEQTVKNHLYRMKHKIGAEDRLGIVQVCRLQGFMP"
BASE COUNT          986 a         1167 c         1364 g          839 t
ORIGIN      
        1 gccgcgggca tgcatccgag ccccgtgccg cacgcggata tcgtttcaac caccacgcac
       61 aagacgttgc gcgggccacg cggcgggttg gtgctgtgca aagcggcgtt tgccaaggag
      121 ctggacaagc tgacgtttcc cgggacacaa ggcggtccgc tggtaaacac gattgcggcc
      181 aaagcggtgt gcttgaagga ggcgatggag ccgtccttca aggaatacca gaagcaggtc
      241 gtggccaaca ccaaagccat ggccgcgtcg gtcgcaaagc atgggttccg catcgtcacg
      301 ggcggcacgg acaaccactt gttcttggtc gaagtgcacc cgcgcggcat caccggaacg
      361 gacgccgaaa aggcgctcga ccgcgcaggc atcaccgtaa ataagaattc gattccgttc
      421 gatcccctcc ccccgatgaa aggtggcgga atccgcctgg gcagcccttc ggtcaccaca
      481 cgcggtatgc gtgaaccgga gatggagcag atcggcgcct ggatcgcgga aattctctcc
      541 aacatcggca acgccgaggt cgagcaacgc gtgcgcaaac aagtggcgga acttgccggg
      601 agattcccga tttatgaggc gcggctgcga ggcggcccag cgcgaatcga acacgcccac
      661 gtttagtcca acggaaatcc tctaatctcg acatgaatat cttcaaatcg acgtccgatt
      721 tcgagcactg gttggcgggg caactcccga tcattcggca ggatctcgcc ctcaagcatc
      781 agcacatggc ggaagccgct ttccctttct tccgcgcaac tttttaccgc tggctgcagc
      841 tctggccgga ggtgtgcgct catctggcga aggcccctgc ggtattaggt gtaggcgatc
      901 tgcacatcga aaacttcggg acgtggcggg atgaagaaga acgcctgatt tggggcgtga
      961 acgatctgga tgaagcatgg cccgcgccgt acgcgctcga tctggtgcga ctgatcacca
     1021 gcgtttatgt tgcgatcgag gaagaacacc tgtgccttgc aaagcgggtg gctgccgaag
     1081 ccatcgaaga aggatatcga gacgccctgg cagcgggcgg caaaccgttt atcctggcgg
     1141 aagaccatcg ctggctgcgc ttgctcgccc tgggcaaact gcgcgacccg gtacaattct
     1201 gggacaagct ggagcacctg ccgccataca ccgcaaaacc accggaggaa gcgcgcaagc
     1261 tgctggaaaa atcgtttcct attgcactgg aagaatatct attaaaaaag aggatcgccg
     1321 gtctgggcag cctcgggcac cccggatact cgccttgtcc agatggagag gagcgttgat
     1381 cgcccgggaa gcaaaggcca ttcgtccttc ggcgtgagtt tgggccagga aggcatcttc
     1441 cgcggaaatc tattgcgaag agctgatgca gcgctcgatt cgcgtgccgg atccctgcgt
     1501 gcgcttccac gggcgctggg tggtgcggcg tctggcgccg gactgctcgc gcattgaact
     1561 cgcttccctc tcgaaggatc gcgacgaggc gcggctgctc tattccatgg gctgggaaac
     1621 cgccaacatg catttcgcca cgccgcgggc gatggccaag gtggagcatg accttgcggc
     1681 tcggcggggc cgctggctcc ataaggcggc aaaggccatg ctggcggtaa ccgcgaaaga
     1741 ttggaaaaag tggcagcgcg attggaagcg ctccgcgccg cgttagacgc tggcgcagga
     1801 cctgtcctca ttcttgacgc tccaactaga gtgccgctgg cccaagactg tcctccgggt
     1861 cagtagattc tgaataaaac tgcgagtagc ttcaaaaatg actgggatgg gaagcctact
     1921 cgcgaccgtg ttactcgctg ttccgctcat cgcctggctg agcgggacat ttcttcttga
     1981 tgattcacaa cttaagttgg cgcgattctc caacgcgccg cgcgggatct tcccagtctc
     2041 ccggaaatcc tattctcctg caatcgcctc gcgcccggtt tctcatcggg ccgcccggcg
     2101 gtggatgagg tgagccgatg atggatacgg aagccttccg tgaattcaag actggattaa
     2161 cactgctgcg agacaattac gccataaaag ctctgccgca catgcgacgc gccgtggatt
     2221 tggataaaaa caatccttat tacatgtcct acctgggcgt ggtgctggcg cgctcggaag
     2281 gaaagtgggg cgaagcggaa aggctgtgcg actccgcggt tcgaatgaag cgcaatcagg
     2341 cgcagctcta cttgaatctc gctgaagtgt atgccaccgc tggacggcgt gacgacgccg
     2401 ttgacgcttt ggagaccggg ctgaaatatg cgcgccgcga tatccggctg acgattgcga
     2461 tgaacaaact aacggcgcgc cggcctcccg tgctgagttt cctcggccgc agacatccac
     2521 tcaaccggca gctaggaata ctaaggcacc gcaccatgcg gatatttggg cagccgtctt
     2581 agatcgatct ctccaccccc aaacacaaga acgggcgtcc cggatgatcg ggacgcccgt
     2641 tcttgctttt gtcggcttgg cggaatggct gtgcggtctg cgcaaactaa cgcgagttgc
     2701 cagcgccaaa gttataagag tgttgcattc ccatttccat gaggatcttg aacccaatgc
     2761 cgaacatgcc ggtggggaga acgtcccttc gaacgacttc cgcgagatac tcgcgggcca
     2821 tggaagcggg atcttgtgaa tacggcatgg ttacgaagag ccgcatgcca atctcataac
     2881 cagattcact ggactggaaa aagacaccgg agcgggaaac gctgaccgag ccgcgcatgt
     2941 cttcgaagtg gtctttgttc ggatcggaag gacggatgcg cataagctgg gtaatcttgc
     3001 agcggcggct gccgcggcgc tccgtatttg aaatcgccga ctttggggaa cttcctgttg
     3061 agagtttttt cgtcatcaga tcttgctggg tcgccatggg taaaccatct ttccggaaaa
     3121 atgctgcgct ctatccgaga agagtgtatc ccgagggcga caactaaacc tatagcggca
     3181 acgtacccag gaggaagtag ggcgctccga gcaaatggta gtacggaagt actagaaaag
     3241 gaagggaggg ggtgtggaaa aagagcggta aagtgctgat agagaagaag aagagagaaa
     3301 gtggttccaa aatgaggctg aaggccaggt ttggtaccaa ttcgagacta gggaggggga
     3361 gagttttgca cagatgagaa cttaaatggt tctttttatt ggggattgac agcggatggt
     3421 cggaatgagg agggatgaaa gcgcggctgc aaaattgcag aagtgaagtg tacgcgtggg
     3481 gttccgacaa atcgctaatc atgacgcctg gcctgtgcat gagagcggag aaaactggag
     3541 gatgtcccgc gatgagtaca gcaacgcgcc agattttgca ggtaggagaa caagcccaaa
     3601 agagaatcaa ttcgaatggt gatcgccgga caaacatcgc ggtgggggaa agtgccgccc
     3661 gcgtaagcgg cggagcggtg gtggaacgga agccacgagt ctttgtggcc gctgagaatc
     3721 gcctgctgag agaggcgctt tcccggatgc tggtcaagag cggggaggtc gaagtgatcg
     3781 gaacggacat ggcggaacca ttccggacgg aggacctgct caaagaagaa acggacatcc
     3841 tgctcctgtc atcgcgcgga aacagacacg aagatctgac cgcagttcga agggtacgga
     3901 cgaccgctcc caaagtgcaa attcttctga ttggcgtgac gggagaggaa gccgagtttc
     3961 tgcagtgcgt gcgggcggga gttcgcgggt acctgccgcg agacgcttcg gcggaggacg
     4021 tggtggaagc cgtgagagcg ctgcaaggag gcgaagcgac ctgccccggc acgctttgcg
     4081 caacgctgtt ccggtatttc gaacgagaag cgacctcctt cccttctgcg agcgcacatc
     4141 agcggctggg attgacgcgg cgcgaacagc agttgattcc cctgattgcc gaggggctca
     4201 cgaacaagga aatcgccaat cgcttctgtc tttcggagca aacagtgaaa aaccacctct
     4261 atcgaatgaa gcacaaaatc ggcgcggagg atcgcctggg catcgtgcag gtttgccggt
     4321 tgcaggggtt catgccgtag tttttgggtt ctcgtt
//
LOCUS       DASYVO010000361         9466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_94504, whole genome shotgun sequence.
ACCESSION   DASYVO010000361 DASYVO010000000
VERSION     DASYVO010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9466
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94504"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VGR03_12160"
     CDS             <1..563
                     /locus_tag="VGR03_12160"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV1995076.1"
                     /translation="GAAVLLLALLMVSWLGVGEVLQRFSSFQSLEATAGKRASMRRDT
                     WQIFLHHPVTGTGLGTLQIVYPPYESLYDGKIVNHTHNDYLEALAETGILGGLCCAWF
                     LGVLFSESLKRLRQLNNSFAGALQLSGLVACTGFLVHALVDFNLHIPANALLFFLMAH
                     LATSEIQSVAPPSAPQRPRHRNSRNH"
     gene            615..1790
                     /locus_tag="VGR03_12165"
     CDS             615..1790
                     /locus_tag="VGR03_12165"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEV1995077.1"
                     /translation="MQPARLGALVWYFVPTALLVLLVLAPSGSAQQAVLETPQQVNDR
                     IRSMSASARLMPHDYVIGNGDLLNLEVFDVKELSREVRVSQTGSIGIPLLPVRLHISG
                     LTEVQAEQKISEVLESNGLVSHAEVSLSVKERKSKPITVVGAVGHAMVFQADRPVTLL
                     EVLAEAGGVANDAGDTVIVNRPAQDAPSDPSGPPAIGPEDTAPAVASKGSIHEGSTQE
                     TASSTIAVPNASANTLAPGDSPHLNNTITINLNELMESGDATNNIILQAGDIVTVPHS
                     GIVYVLGAVSRPGGFVLANDRGQMTTLKILALAGGLSPTAKRDHAVIVRKDAQGQQHE
                     VAVDLKKVMERQAEDVQLQPSDILIVPNSPGKQAMLRALEFGIAIGSGVALYRVAYH"
     gene            1793..4132
                     /locus_tag="VGR03_12170"
     CDS             1793..4132
                     /locus_tag="VGR03_12170"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEV1995078.1"
                     /translation="MADENKLVPRENGSNGAVELVSSPARFPAFELSPREPHLYDYLL
                     ILRKHQWLILSFMLAVVTIVAIATFRMQPVYVATARVEIDRENANILPFQGTDSYDFM
                     MDLENYIETQSKILTSETLALQTIRNNALSARPEFASPNGPSEAIASGSLANQKRPPE
                     LGEFLGSLSVKRVPNSRLMDVSFESTDPLLAARIVNAHITSYIEQNFRSKYEATSQAS
                     TWLTGQLDDLRVKVQKSEDALITYERQNQIWTLDDKQNITTQRLADVNRELTDAQSER
                     MRKESLYQFAKSGNLDAVPQVQSNTTLMELLRRHADLSSQYNDAVSQYGPNFPKVQRL
                     QAQLKDLDDNVEKEKQKIVGILESDFREARERETMLTGALDLQKVAANQMAEKLVEYN
                     ILKREAEANKTLYEGLMTKLKETAISASLRSSNIRVVDPAMIPSTPARPAKTRNVALA
                     FLVGLVGGIGLALMREYLDNTVKTPDDVETLSRLPSLAVVPQFAGSNGSGKRHGLLQG
                     FSSNNGHDKRIELVAQHLPKSQMSEAFRALRTSILLSQADHPPQVILVTSALPREGKT
                     TAAANLAVTLAQLGDRTVLVDADLRKPGVGRLLNLASGKYAGLSSYLAGVSSLDLVSV
                     PHPAIPNLVAIPTGPLPPNPADLLSSHKLVEAIAELRTKFKFIVIDSPPIMAATDAVI
                     LSVQADGVLLVVRSGETPKEAFTRTRDLLNSVKCHILGVVLNAVDASAPDYYYSYRYY
                     PYSYGYGPQEAAEAPHEDDDSEAVTERVSRDRDDDSQAL"
     gene            4142..5149
                     /locus_tag="VGR03_12175"
     CDS             4142..5149
                     /locus_tag="VGR03_12175"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEV1995079.1"
                     /translation="MRYLITGGAGFIGSHLAERLLDRGDRVVLLDNLSTGSVENIRHL
                     KSSDRMQYHLDNIENRQLLAELVDDADVIVHLAAAVGVKLIVESPVKTIETNVNGTQM
                     ILEAACKKKKLVLTASTSEVYGKNTNVPFHEDADLVLGPTTKGRWSYAASKALDEFLA
                     LSYWKEKKLPVIVVRLFNTVGPRQTGRYGMVLPNFVKSALDNTAISIYGSGKQSRCFC
                     DVRDTVEGLIRLMDTDRSIGEVINVGNTEEITIEVLAQRVKERTGSSSPIEYVPYDQA
                     YEPGFEDMMRRVPCVEKLYALTGFRPQTPLNEIIDRVAAFFQQKAEALDIHRVATTSA
                     D"
     gene            5295..7889
                     /locus_tag="VGR03_12180"
     CDS             5295..7889
                     /locus_tag="VGR03_12180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1995080.1"
                     /translation="MTKTGNEGQRRMNLIGWLDTVLCDLKYTFRMLARTPGFTAVAVL
                     TLALGIGANTAIFTLLDQVLLRLLPVKDPQQLVLLTMKGRHYGSNWGGNAISHPMFRD
                     FRDHNEVFSDMFCRFPTSASLSFGQQAERVSVELVSGTYFSTLGVIPAMGRILTPEDD
                     RVPSGHPFVVLNYNYWKTRFAADAQIVGKTLTLNNYKMTVVGVAQAGFDGVELGFSPK
                     IFIPIMMQAQTIIGNPEDMLKERRNRWVNAFGRLKPGISQQQAKAALQPFMHSMLESE
                     VQLPAFSRASKYDRDEFLKCWIDVLPGSQGRSYTRRQLSTPLWVLMATTGAVLLIACA
                     NLANLMLVRGSSRVKEIAVRLAMGATRPRIISQLLIESLSLSALGGVTGLAVAFWADR
                     ALMKIYLPSDSGGMNISSLPDLRILLFNLTVTVLTGVIFGLVPALQTTKPNIAGTLKD
                     QAGAVVGGGGHHRLRKALVIAQVTLSLLLLVGAGLFVRSLANLRNLGPGFSPERLVGF
                     NIDPSLNGYTPERLKVFYPQLSDALRAIPGVRSVGLASMRILEDNEWDSSMSVEGFTP
                     AKPDEHAEPYMNSIGPNYFATLGVPVVAGRDFRINDNHEVKHGPEAWNWAPTTIMINE
                     KFAKKFFPGRNPIGLHVGFGTDPGTATDMEVIGVVKDFKYTNLRDEIPEQAFVPYLAS
                     RYLGDMTIYLRTTGDPNQLMPAVRSKVRDMDSNLPVYAMRTTEVQINNSLSTERMIAS
                     LSAVFGFLATLLAVIGLYGVMAYTVAQRTREVGIRMALGAARGHVIWMVMREVLLLVA
                     IGVAAGVTASLALTRVIQSQLFGLTPHDPLTLGLATAALALVACAAGYVPALRASRLD
                     PMAALRYE"
     gene            complement(8114..9403)
                     /locus_tag="VGR03_12185"
     CDS             complement(8114..9403)
                     /locus_tag="VGR03_12185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV1995081.1"
                     /translation="MTATDTHRAIEAVWRIESARIIASLARIVRDVGLAEELAQDALV
                     AALQQWPESGVPDNPGAWLMATAKHRAIDMFRRNKRLQRKHEELGRELQTKQEMAVPD
                     FNAALDDDIGDDLLRLVFISCHPVLSTDARVALTLRLLGGQTTDEIARAFLAPEPTIA
                     QRIVRAKRTLAEERVPFEVPRGAELAARLSSVLEVIYLVFNEGYSATSGGDWMRPALC
                     EDALRLGRILAELASQEPEVHGLVALMEIQASRSRARVSSSGEPILLLDQNRAHWDQL
                     LIRRGLAALERAQRLGGMQGPYALQAAIASCHARALTPEETDWARIVALYEALGSLTP
                     SPVVELNRAVAIAMAYGPAAGLELVDALTEEPSLKDYHLLPSVRGDLLKKLGRFDEAR
                     AEFERAAGLTRNARERELLLDRARACAGSSAPPAEPR"
BASE COUNT         1841 a         3125 c         2527 g         1973 t
ORIGIN      
        1 ccggcgctgc tgtcctgctc ctggcgctgt tgatggtttc ctggctcggc gtcggagagg
       61 ttctgcagcg tttttcttcg ttccagtccc tcgaagccac cgccgggaag cgggcctcca
      121 tgcgccggga cacctggcaa atcttcctcc atcaccccgt taccggaacg ggactcggca
      181 cgttgcagat cgtttatccg ccttacgaat cactctacga tgggaaaatc gtcaaccaca
      241 cgcacaacga ttacctcgag gccctcgccg aaaccggcat tctgggaggg ctctgttgtg
      301 cctggttttt gggtgtcttg ttcagcgaat ccctgaaacg attgcgccag ctcaataatt
      361 cctttgccgg ggccttgcaa ctttccgggc tggtggcttg caccggcttc ctcgtgcatg
      421 ctctggtcga tttcaacctg cacatccccg ctaacgctct cctgtttttt cttatggccc
      481 acttggcgac atccgagatc cagtctgtcg ctcccccgtc tgcaccccag cggccgcgcc
      541 atcgcaattc tcgaaatcac tgattgattt gggcgttctc cctgtataat cacacttggg
      601 ggtcatctat tctcatgcag ccagccagac tgggtgcgtt ggtgtggtat ttcgttccga
      661 ctgccctgct cgtgttactg gttctggcgc cttccggcag cgctcagcag gccgttctcg
      721 agacgccaca gcaggtcaac gaccgcatac gctcgatgtc cgcctccgcg cggttgatgc
      781 cccatgacta cgtcatcggc aatggcgatc tgctgaacct cgaagttttc gatgtaaaag
      841 aactcagccg tgaagtgcgc gtcagccaga ccggatccat cggcatcccc ctcctgccgg
      901 ttcgcttgca catctctgga ttaacggaag tccaggccga acagaagatc tccgaagtcc
      961 tggaatccaa tggcttggtc tctcatgccg aagtaagcct cagtgttaaa gaaagaaaaa
     1021 gcaagcccat caccgttgtt ggggctgttg ggcatgccat ggttttccag gccgaccggc
     1081 ccgtgaccct tctggaagtc ttggcggagg ccggcggcgt tgccaatgac gccggcgaca
     1141 ccgtgattgt gaaccgtccc gcccaggacg cgccgtccga tccatcaggg cctccggcga
     1201 tcggcccgga agatactgct cccgccgttg cctccaaagg atcgatccac gaaggctcga
     1261 cgcaggagac cgcgtcctct acaattgcag tccccaatgc ctcggcgaat actctcgcgc
     1321 cgggtgattc acctcacttg aacaatacga tcaccatcaa tctgaacgaa ctgatggaaa
     1381 gcggcgacgc cacgaacaat attattctcc aggccggcga tatcgttacc gtcccgcact
     1441 cgggaattgt ctacgtcctg ggagcggtga gcaggcccgg tggattcgtc ctggcaaatg
     1501 accgcggcca gatgaccacc ttgaaaattt tggctctcgc cggtggtttg agccccaccg
     1561 cgaaaaggga tcacgcggtc atcgtccgaa aagacgctca ggggcagcag cacgaagttg
     1621 ccgttgatct caagaaggtg atggaacgcc aggcggaaga tgttcagctt caacccagcg
     1681 acattctgat cgtgccaaac agtccaggca aacaggcgat gctcagggct ctggaattcg
     1741 gaattgctat cgggagcggc gtcgctctct accgggtggc gtaccactaa aaatggccga
     1801 cgaaaacaaa ctcgtacccc gtgaaaatgg ttctaatggc gccgtcgagc tggtctcctc
     1861 gccggcccgc tttcctgcct tcgagctctc tccccgggaa ccccacctct acgactacct
     1921 gctgattttg cggaagcacc agtggctgat tctctccttc atgctcgcgg tcgtcaccat
     1981 tgttgccatc gccaccttcc gcatgcagcc cgtttacgtg gccactgctc gcgttgagat
     2041 tgaccgcgag aacgccaaca tccttccttt ccaaggcacg gattcctacg acttcatgat
     2101 ggatctcgag aattatatcg agacgcaatc caaaatcctt accagcgaaa cgcttgccct
     2161 ccaaaccatt cgcaacaacg ctctttcggc ccgcccggaa tttgcttcgc ccaacgggcc
     2221 ctcggaagca atcgccagcg gcagtctcgc aaatcagaaa cgcccgcccg aactgggtga
     2281 attcttgggc agtctcagcg tgaagcgcgt ccccaatagc cgcttgatgg acgtcagttt
     2341 tgaatccacc gatcccctgc tcgccgcacg catcgtcaac gcgcatatca cctcctacat
     2401 cgagcagaat ttccgcagta agtacgaggc caccagtcag gcttccacct ggctcaccgg
     2461 ccagcttgac gatctgcggg tcaaggtaca gaagtccgaa gacgctctta tcacctacga
     2521 acggcaaaat cagatttgga cgcttgacga caagcaaaac atcaccaccc agcggctcgc
     2581 tgacgtcaac agggaactca ccgacgcgca gagcgagcgc atgagaaagg aatcgcttta
     2641 ccagtttgcc aagtccggaa atttggatgc tgttcctcag gttcagagca acactacgtt
     2701 gatggaattg ctccgaaggc acgctgatct ttccagccaa tacaacgacg cggtcagtca
     2761 gtatggcccc aattttccca aggttcagcg cctccaggcc caattgaaag atctcgacga
     2821 caacgtcgaa aaagaaaaac agaagatcgt cggcatcctc gaaagcgatt tccgtgaagc
     2881 tcgcgagcgc gaaaccatgc tgaccggggc cctcgacttg caaaaggttg ccgccaacca
     2941 gatggccgaa aagcttgtcg agtacaacat tctcaagcgc gaggccgagg ccaataagac
     3001 tctttacgaa ggcctgatga ccaagttgaa ggaaaccgca atctccgcca gcttacgctc
     3061 ttccaacatt cgtgtggtcg atccggccat gattccttcc accccggcgc gtcctgccaa
     3121 gactcgcaac gtggcgctgg cctttctcgt gggcttggtt ggcggcatcg gtttggcttt
     3181 gatgcgcgag tatctggaca acaccgtgaa gaccccggac gatgtcgaaa cgctttcgcg
     3241 gctgccttcc ctcgcggtcg tgcctcagtt tgccggttcc aacgggagcg gcaagcgcca
     3301 tggccttttg caaggcttct cgtccaataa tgggcatgac aagcgcatcg agctggttgc
     3361 tcagcacttg cccaagtcgc agatgtcgga ggcgtttcgc gccttgcgaa cttccattct
     3421 gctctcgcag gccgatcatc ctcctcaggt cattttggtc accagcgctc tcccacggga
     3481 agggaagacc accgctgccg cgaatcttgc cgttacgctc gcgcagttgg gcgataggac
     3541 cgtgctggtg gatgccgacc ttcgcaaacc cggcgtcggc cgcctcctca atttagccag
     3601 cggaaaatat gcggggctga gttcctattt ggccggcgtt tccagtctcg atctggtgtc
     3661 tgtaccgcac cccgcgattc ccaatctcgt cgccattccc accggacctc ttccgccgaa
     3721 tcccgcggac ctgctttctt cccacaagct tgtcgaggcc atcgccgagc tccgcaccaa
     3781 gttcaagttt atcgtcatcg attcacctcc catcatggcc gccaccgatg ctgtgattct
     3841 ttccgtgcaa gccgatggcg tgttgctagt cgttcgcagc ggcgaaacgc ccaaggaagc
     3901 tttcactcgc acgcgtgacc tgctcaatag cgtgaagtgt cacatcctcg gcgtcgttct
     3961 caatgccgtg gatgccagcg ccccggacta ctactactct taccggtatt atccgtactc
     4021 gtatggatat ggtccccagg aagcggctga agctccgcat gaagatgatg attccgaagc
     4081 cgtcaccgag cgcgtctccc gcgatcgcga cgatgactcc caggcccttt aaggaggccc
     4141 tttgcgctat ctgatcaccg gcggcgccgg ctttattggt tcacacctcg cggagcggct
     4201 tctcgaccgc ggcgaccgcg tcgttttgct cgacaacctt tcgacgggca gcgtcgaaaa
     4261 catccgccat ctcaagtctt ccgaccgcat gcaatatcac ctcgacaaca tcgagaatcg
     4321 ccagttgctc gccgagctcg tcgatgatgc cgatgtgatc gtccatctgg ccgccgccgt
     4381 cggcgtcaag ctcatcgtcg agagccccgt caagacgatc gaaaccaatg tgaacggcac
     4441 gcagatgatc ctcgaagcgg cctgcaagaa aaagaaactc gttctcacgg cgtctacgtc
     4501 cgaggtctac ggcaaaaata ccaacgttcc ctttcacgaa gatgcggacc tcgtccttgg
     4561 ccccaccaca aagggccgct ggagttacgc ggcctccaag gcgctcgatg agtttctcgc
     4621 tctttcctac tggaaggaaa aaaagctccc cgtcatcgtg gttcgcttgt tcaataccgt
     4681 cggtccccgg caaaccggcc gctatggaat ggtgcttccc aacttcgtaa agtccgccct
     4741 ggacaacacc gccatcagca tctacggcag cggaaaacaa tctcgctgct tctgcgatgt
     4801 gcgtgacacc gtcgaaggtc tcatccgtct catggacacg gatcgttcca tcggcgaggt
     4861 catcaacgtc ggcaacaccg aggaaatcac cattgaggta ttggcgcaga gagtgaagga
     4921 gcgcaccggc agttcctctc ccattgagta cgtgccttac gaccaggcct acgaacccgg
     4981 ttttgaggac atgatgcgcc gcgtgccctg cgtcgaaaag ctctatgccc tcaccggctt
     5041 ccgtcctcag actccgctga atgagatcat tgaccgcgtc gccgctttct ttcagcaaaa
     5101 agcggaagcc ctggacattc atagggtagc cacgaccagc gccgactaaa ttccgtccgc
     5161 tggccggatc gtgccgtacc ctccgaatag tgttcgcttt ttattccccc taacaaccca
     5221 cgaatctttt cgtactttca tcaaactttt cgctactttc ttcgtctcta cctctagctg
     5281 cccgggggtt acgtgtgacc aagaccggca atgaaggcca acgccgcatg aatctcatcg
     5341 gctggctcga caccgtcctt tgcgatctga agtacacctt tcgcatgctc gcccgcactc
     5401 ccggcttcac cgccgtggcc gtcctgacct tggctctcgg catcggcgcg aataccgcca
     5461 ttttcactct cctcgaccag gtcttgttgc gcctcctgcc cgtcaaggac ccccagcaac
     5521 tcgtcctgct caccatgaag ggacgccact acggcagcaa ctggggtggc aacgccatct
     5581 cccatcccat gttccgtgat ttccgcgatc acaacgaagt cttctcggat atgttctgcc
     5641 gctttcccac ttccgccagc ctttcctttg gccagcaggc ggaacgcgtg tccgtggaat
     5701 tggtttccgg cacctacttc tccactctcg gcgttatccc cgccatggga cgcatcctta
     5761 ctcccgagga cgatcgcgtc cccagcggcc atcccttcgt cgttctcaac tacaactatt
     5821 ggaagacgcg ttttgccgcc gatgcccaaa tcgtcggcaa aactctaact ctgaacaatt
     5881 acaaaatgac cgtcgtcggc gtcgcccagg caggttttga cggcgtcgag ctcggcttct
     5941 ccccgaaaat cttcattccc atcatgatgc aggcgcagac catcatcggc aacccggaag
     6001 acatgctgaa ggagcgccgc aatcgctggg tgaacgcctt cggccgcctg aagcccggca
     6061 tctcgcagca gcaggccaag gccgctctcc agcctttcat gcactccatg ctcgagtcgg
     6121 aagtgcagct cccagccttc agccgcgcct cgaagtatga ccgcgacgaa tttctaaaat
     6181 gttggatcga cgtcttgccc ggctcccagg gccgctccta tacgcgccgc cagctctcca
     6241 ctccgctctg ggttttgatg gccaccaccg gagccgtcct gctgatcgct tgcgccaacc
     6301 tcgccaactt gatgctcgtg cgcggctcca gccgcgtcaa ggaaatcgcc gttcgtttgg
     6361 cgatgggcgc cacccggccg cgcatcatca gccaactttt gatcgagagt ctttctctct
     6421 ccgcgctcgg gggagtcacg ggtctcgccg tcgccttttg ggccgaccgc gccctcatga
     6481 aaatctacct gccctccgat tccggcggca tgaacatttc cagcttaccc gaccttcgca
     6541 ttctcttgtt caacttgacg gtgaccgtct tgaccggagt catcttcggc ctcgtgcctg
     6601 ctcttcagac caccaagccc aacatcgccg gaacattgaa ggatcaggcc ggcgccgtcg
     6661 tgggcggcgg cggccaccat cgcttacgca aggccctcgt catcgcgcaa gtcacgcttt
     6721 cccttctgct cttggtagga gcgggactct ttgttcgcag tcttgcgaat ctgcgcaatc
     6781 tcggccccgg cttttcccca gagaggctcg tgggcttcaa tatcgacccg tctctcaacg
     6841 gctacactcc cgagcgcctg aaagttttct atccccagct ctccgacgct ctccgggcga
     6901 ttcccggagt tcgatcggtg gggctggctt ccatgcgcat tctcgaagac aacgaatggg
     6961 acagcagcat gtccgtcgag ggcttcactc ctgccaagcc cgatgagcac gccgaaccct
     7021 acatgaactc catcggcccg aactattttg ccaccctggg cgtgccggtc gtcgccgggc
     7081 gcgacttcag gatcaacgac aaccacgaag tcaaacacgg tcccgaggcg tggaattggg
     7141 cgcccaccac gatcatgatc aacgagaagt ttgccaagaa gtttttcccc ggacgaaatc
     7201 ctatcggcct tcatgtcggc ttcggcaccg atcccggcac ggcgaccgac atggaggtca
     7261 tcggcgtcgt caaggatttc aagtacacca atctccgcga cgaaattccc gagcaggcct
     7321 tcgttccgta tcttgccagc cgctacctcg gcgatatgac catctatctc cgaaccaccg
     7381 gcgatcccaa tcagttgatg cctgccgtgc gctccaaagt ccgcgacatg gattccaatc
     7441 tgcccgtcta cgccatgcgc accaccgaag tacagatcaa taactccctt tccaccgagc
     7501 gcatgatcgc cagcctctcc gccgtcttcg gcttcctcgc cactctgctc gcggtcatcg
     7561 gtctctacgg cgtcatggcc tacaccgtcg cccagcgcac ccgcgaagtc ggcattcgca
     7621 tggccctcgg tgccgcccgc ggccacgtca tctggatggt catgcgcgaa gtcctcctcc
     7681 tcgtcgccat cggtgtagcc gccggcgtca ccgcctccct cgcgctaacc cgcgtaatcc
     7741 aaagccagct cttcggcctc acgccgcacg atccgctcac gctgggtctt gccaccgccg
     7801 cactcgccct cgtcgcctgc gccgccggtt acgtccccgc cctccgcgcc agccgcctcg
     7861 accccatggc tgccctccgc tacgaataag ccttggtttt ttcagaatca caacgaaaaa
     7921 caaaagtagc cgtgagcggt ccaacgcgtc ttctcactga ggacaagatt ttccagttcg
     7981 atctgacgtg ggctgaaggc acttctacga gtcgcgcgta gtacgagaag ctcgatggtt
     8041 tccgagtatg ccgtcactca accgcgatca gctcgctcgc agcgcgtgca aagcgcgaga
     8101 cgcgccgctt tcgtcagcgg ggctccgccg gtggcgccga gctgcctgcg catgctcggg
     8161 cgcgatcgag cagcagctcg cgctcgcggg cgttgcgcgt gagtcctgcc gcgcgctcga
     8221 actctgcgcg ggcctcatcg aagcggccga gctttttcag taagtcgccg cgcacgcttg
     8281 gcaagaggtg gtaatccttg agcgacggct cctcggtcag tgcatccacc agttcgagtc
     8341 ccgccgctgg accgtaagcc atggcgatcg caaccgcccg attcaactcc acgacaggcg
     8401 acggtgtgag actgccaagc gcctcgtaca gcgccacgat gcgtgcccaa tccgtttcct
     8461 ccggggtcag cgctcgtgcg tgacaagagg cgatcgcagc ctgcagtgcg tacggaccct
     8521 gcataccgcc gagcctttga gcccgttcga gcgcggccag accgcgacgg attaggagtt
     8581 ggtcccagtg cgcgcggttt tggtcgagca gcaggatggg ctctcccgac gagctcacgc
     8641 gcgccctgga gcgcgacgcc tggatctcca tgagcgcgac aaggccgtga acctccggct
     8701 cctgcgaagc gagctcggcc aggatacgcc cgagacgcag cgcgtcctcg cagagtgcag
     8761 gccgcatcca gtcgccaccg gaggtggccg agtatccttc gttgaagacg aggtagatga
     8821 cctcgagcac cgacgagaga cgtgcggcaa gttcggctcc gcgaggaacc tcgaagggga
     8881 cgcgctcttc ggcgagggtc cgcttggcgc ggacgatgcg ctgcgcgatg gtcggctccg
     8941 gtgcgaggaa ggcacgggcg atctcgtcgg tcgtctgacc accgagcaag cggagcgtga
     9001 gcgccacgcg cgcgtcggtc gagagaacgg gatgacagga tatgaagacg aggcgcaaga
     9061 ggtcgtcgcc aatgtcatcg tcgagggcgg cgttgaaatc cggcacagcc atctcctgct
     9121 tggtttggag ctcgcggcca agctcctcgt gcttgcgttg aagccgcttg tttcggcgga
     9181 acatgtcgat cgcacggtgc ttcgcggtgg ccatcagcca ggcgcccgga ttgtccggga
     9241 cgcccgactc cggccattgc tggagcgcgg cgacgagagc gtcctgcgcg agttcctcgg
     9301 cgaggccaac gtcgcgcacg attcgagcga ggctagcgat gatcctggcg gactcgatcc
     9361 tccaaaccgc ttcgatggcg cgatgtgtgt cggtagccgt cacagccagc gatcagaaca
     9421 tttttgatcc tccggcacaa gaccgaggct cgccactggc cgtcct
//
LOCUS       DASYVO010000362         5802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_167584, whole genome shotgun sequence.
ACCESSION   DASYVO010000362 DASYVO010000000
VERSION     DASYVO010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167584"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VGR03_12190"
     CDS             <1..537
                     /locus_tag="VGR03_12190"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEV1995082.1"
                     /translation="DLIIYSAGLPEHRETVRLALREHGRHAASPMAGVKSISWLNNVW
                     AVAEAVKEGFDEVVMLNERGEVAECTSANIFAVKNEKILTPPLNSGCLEGVTRGILME
                     IAPEAGVSVLEQALRPEDLYAADEVFISSTNRNLISVGEIAGHKIPHPGPVCNRLNDL
                     FDAYVTDYVSRRLASAVR"
     gene            583..1866
                     /locus_tag="VGR03_12195"
     CDS             583..1866
                     /locus_tag="VGR03_12195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1995083.1"
                     /translation="MASDTNLTAIRATAPADTGEKTVFKILLAISFCHLLNDTLQSLI
                     PAIYPLLKNSFHLDFGQVGLIALTLQLTASILQPLVGLYTDRRPQPYSLAIGMGFTLA
                     GLLAFALAPSYGTILAAAALMGIGSAVFHPESSRVARMASGGQHGTAQSLFQAGGNAG
                     SALGPLLAAFVLTKGQSSLAWFSFLAFFGIILLANIGTWTKHHKTHALKSITLDLARL
                     AAMPPAPAHAALPAGKIALSLAILVALMFSKFFYLASLMSYYMFYLMGKFQVSLQSAQ
                     LHLFIFLGAAAAGTIIGGPVGDKIGRKYVIWVSILGVLPFTLLLPYCNLFWTSVLSVI
                     IGLILASAFSAILVYAQELLPGRIGAVSGLFFGFAFGMGGIGAALLGKLVDLTNLNFV
                     YQLCSFLPAIGILTAFLPNLEPARIKTSAPQKSTA"
     gene            1933..2193
                     /locus_tag="VGR03_12200"
     CDS             1933..2193
                     /locus_tag="VGR03_12200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEV1995084.1"
                     /translation="MTTKLTLDKAGRVVLPKSLRDQMQLGPGDTLQVESEGERITLRP
                     VRPRATLKKECGIWVYQGDSTETSIPDLIDREREKHLRESRE"
     gene            2190..2615
                     /locus_tag="VGR03_12205"
     CDS             2190..2615
                     /locus_tag="VGR03_12205"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEV1995085.1"
                     /translation="MKEFFDTSVLVAAFWAGHVHHQASVTRLGAANKKQSACGIHSVA
                     EVYAVMTALSVKALIPPEQALLFVEEVRDRLTLVALESEEYFAAIQKTAGRGFSGGRV
                     YDALLLACAAKAKAQTIYTWNLKHYQALAPELSARILTP"
     gene            2673..4472
                     /locus_tag="VGR03_12210"
     CDS             2673..4472
                     /locus_tag="VGR03_12210"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEV1995086.1"
                     /translation="MPDTAPLAGEAVLEVVSPDGARRYVRITQTPFMIGRGAETGNHL
                     QLSDRRISRNCAAVVIEANRFYVEDRGQRRGLFVNGEKVESRELQDGDAISFGLDDSY
                     EIIFRSAKSSDDDSLPQLLTRMEHITSSEPTGGGLAKLKRLLEATALLHSQLPLDEAL
                     SHMLDHAVSVTDADRGLLLEKDSTDALKVKLARRSGALRLPPESVAPSQTAIQLALKQ
                     QSPVITEDLAQADMDLQAAQSIVAQRLRAVVVIPLFAISRANTQESMVNIKRGELLGV
                     LYLDSRRPAAFSKLDRQILDALAADAANILDNARLVERERERQRMEQEINIARDIQQA
                     LLPRDFRKYPHLAVTGFNLPCLSVGGDYFDVFPLSDGRTAFLIADVSGKGLGAALLTT
                     MLQGALSGMTLGTDPARVFNHVNRFLCDHSEVGRYATMFFGILDQEGHLEFINAGHPS
                     PFLIRRGVAEEAFTEGSYPVGLVPEAEYTAVCLKLEPGDTLVLFSDGVTEAMDPDEQM
                     FGIPRLKELLTGQLECPLEQLQKCVLEAVENFSRGAHQADDLTLLIVRYRATAAAATT
                     DTDVPLSASSSASAAASSASPPASTPPASAASG"
     gene            complement(4645..5217)
                     /locus_tag="VGR03_12215"
     CDS             complement(4645..5217)
                     /locus_tag="VGR03_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995087.1"
                     /translation="MKRNFLAVLIATCVVLLLLTVGTGRAAAQGPAGAGASTAAQGNS
                     NSSGSHSSHSLNPIKWVKKDKNSKNSADSNGNRSDIEKKLTPKLQAQGILPANSNATD
                     ACAPFTALNECLATLHASHNLGVEFNCLRANVTGVQTNADLSGCKGAIGDKAQNLNKA
                     IHELNPSADAKGATKNAEQQAKDDLKDLGA"
     gene            complement(5449..>5802)
                     /locus_tag="VGR03_12220"
     CDS             complement(5449..>5802)
                     /locus_tag="VGR03_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995088.1"
                     /translation="SCMSTFGKPTGDTRRCVFCGKHMAAHLTQCPFCREAVPQVQVSR
                     RTGSNGRRQIRHGLLYMLLGAVIHYFAGGYGAPFFQIPLPAPVMPLVTQYLTPLLFLG
                     GLGFTVYGLFLRIRS"
BASE COUNT         1099 a         1956 c         1494 g         1253 t
ORIGIN      
        1 gacctcatca tttactccgc aggcctcccg gagcatcgcg aaaccgtgcg cctcgccctc
       61 cgcgaacacg gccgccacgc cgcttctccc atggccgggg taaagagcat ctcctggctc
      121 aacaatgtct gggctgttgc cgaagctgtc aaagaaggtt tcgacgaagt cgtcatgctc
      181 aacgagcgcg gcgaagttgc cgaatgcact tccgcaaata tcttcgccgt taagaacgag
      241 aaaattctca cgccgccgct gaactccggc tgcctcgaag gcgtcacccg cggcatcctc
      301 atggaaatcg caccggaagc cggcgtttcc gtcctcgagc aggcgcttcg tcccgaagac
      361 ctctacgccg ccgacgaagt ctttatctct tccacaaacc gcaatctcat cagcgtcggt
      421 gaaatcgccg gccacaaaat cccccatccc ggccccgtct gcaatcgcct gaatgatctt
      481 ttcgacgcct acgtcaccga ctacgtttcg cgccgcctcg cctcggcagt acggtaagaa
      541 cgatgtggcc cttcaccaat ggttcaggga ctccggctct agatggctag cgacacaaac
      601 ttgactgcaa ttcgagcaac cgctcctgca gacactggcg agaagaccgt cttcaaaatt
      661 cttctagcca tcagcttctg ccacctcctg aacgacacgt tgcaatcact aattcccgcg
      721 atctatccgc ttctcaagaa ctccttccat cttgatttcg ggcaggtggg cctgatcgcc
      781 ctcacgttgc aattgaccgc ctccatcctc cagcccctcg ttggtctcta taccgaccgc
      841 cgcccccaac cgtattccct ggccatcgga atgggcttca ctcttgctgg tctgttggca
      901 tttgcgctgg ctcccagcta cggcacaatt ctcgccgccg cggcgctcat gggtatcggc
      961 tccgcggtat ttcatcccga gtcttcccgc gtcgcgcgca tggcttccgg cgggcagcac
     1021 ggcacggcgc aatcgctttt ccaggccggc ggcaacgcgg gctccgcgct cggccctctc
     1081 ctcgccgcct tcgtgcttac caagggacaa tcgagtctcg cgtggttttc ctttctggct
     1141 tttttcggga ttatcctgct cgcaaacatc ggcacctgga cgaaacatca caaaactcat
     1201 gcattaaaat ccatcaccct cgacctcgcg cgtctggccg caatgcctcc ggcgcctgcc
     1261 cacgccgctt tgcccgccgg caagatcgcg ctctctcttg ccatcctggt ggctctgatg
     1321 ttttcgaagt ttttctacct ggccagcctg atgagctatt atatgtttta tctgatgggc
     1381 aaattccagg tttctctcca aagcgctcag ctccaccttt tcatttttct cggcgccgct
     1441 gccgccggca ccatcatcgg cggcccggtg ggcgataaga tcggccgcaa atacgtcatc
     1501 tgggtttcca ttctcggcgt tctgcctttc actttgctcc tgccctattg caatcttttc
     1561 tggaccagcg ttctcagtgt catcatcggc ttgatcctgg cctccgcctt ttccgccatc
     1621 ctcgtctacg ctcaggaact cctgcctgga cgaatcggcg ccgtctccgg cctattcttc
     1681 ggcttcgcct ttggcatggg cggcattggt gcggcgctgc tcggcaaact cgtcgatctc
     1741 accaatttga attttgtcta ccagctctgc tccttcctcc ccgccatcgg gattctcacc
     1801 gcctttttgc cgaacctcga gcccgcaagg attaaaacat cggcgccaca gaaatccacc
     1861 gcctgacatc gaccgtgcat cttcgtcatc aatgccattg acagttggtt ccacccactt
     1921 cataatggca ttatgaccac caagctcacc ctcgacaagg ccggccgcgt ggtcctgccc
     1981 aaatccttgc gcgaccaaat gcagctcggt ccgggtgaca cccttcaggt ggaaagcgaa
     2041 ggcgaacgca tcaccttgcg ccctgtccgc cccagagcca cgctaaagaa agaatgtggc
     2101 atttgggttt accaaggtga ctcaacggag acttccatcc ccgatttgat cgatcgcgag
     2161 cgcgaaaagc acctgcgcga gtcgagggaa tgaaggaatt tttcgacact tccgttttgg
     2221 tcgccgcctt ttgggccggt cacgtacatc atcaggccag cgtcactcgc ttgggagcgg
     2281 caaataagaa gcaatccgcc tgcggcattc attctgtagc cgaagtctat gcggtaatga
     2341 ccgcactatc tgtcaaggcg ctgattcccc cggagcaggc ccttctattt gtcgaagaag
     2401 ttcgggaccg attaacactc gtcgcgctcg aaagcgaaga atacttcgcg gccatccaga
     2461 aaaccgccgg gcgcggcttt agcggcggcc gcgtctacga cgccctgctg ttggcctgcg
     2521 ccgcgaaagc caaggcccag accatctata cctggaatct gaagcattat caggcgctcg
     2581 ccccggagct ttccgcccgc atccttactc cttgacgcga cacctacctc cctatctcgt
     2641 ctcgcatgct acaattccgc accgagtggc ccatgccaga caccgctccc ctagccggtg
     2701 aagcagttct cgaagtcgtc tccccggacg gcgcgcgccg ctacgtccgc atcacgcaaa
     2761 cgcctttcat gattggccgg ggcgccgaaa ccggcaacca cctgcaactc tccgaccgcc
     2821 gcatctcccg caattgcgcc gccgtggtca tcgaagccaa tcgcttctat gtcgaggatc
     2881 gcggccagcg ccgcggcctt tttgtgaatg gcgaaaaagt ggagagccgc gagctccagg
     2941 acggcgacgc catcagcttc ggcctggacg attcctacga gatcatcttc cgctccgcca
     3001 agagttcgga cgacgattcc cttccccaac tgctcacccg catggagcac atcaccagct
     3061 cggagccgac gggcggcggc cttgccaagc tcaagcgctt gctcgaagcc accgcgttgc
     3121 tgcactctca gcttcctctc gacgaggcgt tgagccacat gctggatcac gctgtctccg
     3181 tgaccgacgc ggatcgcggt ctgcttctcg aaaaggactc gacggacgcg ctgaaagtaa
     3241 aactcgcgcg gcgcagcggt gcattgcgct tgccgccaga aagcgtggca cccagccaga
     3301 ctgccatcca gcttgcgctc aagcaacaat cgccggtgat caccgaagac ctcgcgcaag
     3361 cagacatgga cctgcaagct gcgcagagca tcgtcgcgca acgtctgcgc gccgtcgtcg
     3421 tgattcctct cttcgccata tcacgcgcga atactcagga atccatggtc aatatcaagc
     3481 gcggtgaact cctcggcgtc ctctacctgg attcgcgccg ccccgcggcc ttctccaaac
     3541 tcgaccgcca gatcctcgac gcgctggccg ccgacgctgc caacattctc gacaacgccc
     3601 gcctcgtcga acgcgaacgc gaacgccagc gcatggagca ggaaatcaac atcgcccgcg
     3661 acattcagca ggcgcttctc ccgcgcgatt tccgcaaata cccgcacctt gccgttaccg
     3721 gtttcaatct tccctgctta tcggtcggag gagattactt cgacgtcttt ccgctcagcg
     3781 atggccgcac cgcgtttctc atcgcggacg tttccggcaa aggacttggc gccgcgctgc
     3841 tcaccaccat gcttcagggc gcgctttccg gcatgactct cggcaccgat cccgcgcgcg
     3901 tcttcaatca cgtcaatcgc ttcctgtgcg atcactccga ggttggccgc tacgccacta
     3961 tgttcttcgg tattctcgat caggaaggcc atctggaatt catcaacgcc ggccatcctt
     4021 cgcctttcct gattcgccgc ggcgtcgcag aagaagcttt caccgaaggc tcttatcccg
     4081 tcggcctggt tcccgaagcc gaatacaccg ccgtttgcct gaagctcgag ccaggggaca
     4141 ccctggtgct cttcagcgac ggcgtcaccg aggccatgga tcccgacgag cagatgttcg
     4201 gcatcccgcg cctcaaggaa ctcctcaccg gccagcttga gtgccccctc gaacaactcc
     4261 agaaatgcgt cctcgaagcc gttgaaaact tctcccgcgg cgcccaccag gccgacgacc
     4321 tcacacttct catcgtccgc taccgcgcca ccgccgccgc tgccaccacg gacacggacg
     4381 tcccgttgtc cgcctcatcc tccgcctccg ccgctgcttc ctccgcgtca ccgccggcct
     4441 cgactccccc cgcttccgcc gcgtccggct aaatgccgtt gtgatgcgct tgccgcaaga
     4501 ttccttccgg atgacgctta aattcaaaaa ttaagggcga aggccatgat cgtcggcctt
     4561 cgccctgttt cgatttcgcg gattttttcg accttcgtcg aaaagtctat tcaatcgcgt
     4621 tgagattttt ctctggcttc ggccttacgc gccgagatcc ttgaggtcgt ccttggcctg
     4681 ctgctcggcg ttcttggttg ctcccttggc gtccgcgctg ggattcagct catggatcgc
     4741 cttgttcagg ttctgtgcct tgtcgccgat cgcgcctttg caaccggaca ggtcagcatt
     4801 ggtctgcacg cccgtcacat tcgcgcgcag gcagttgaac tccaccccaa gattgtggct
     4861 ggcgtggagc gtggcaagac actcgttcag tgccgtgaaa ggcgcgcacg catccgtggc
     4921 gttcgaattg gccggcaaaa taccctgggc ctgcagtttc ggcgtcagct tcttttcgat
     4981 gtcgctccgg ttgccgttcg aatcggcgga atttttggaa ttcttgtctt tcttcaccca
     5041 cttgatggga ttaagggaat gggagctatg cgatcccgag gaattggaat ttccctgcgc
     5101 tgcggtggaa gcgcccgcgc ccgcggggcc ttgcgccgcg gctcgacctg tacccacggt
     5161 caaaagcaac aagaccacgc acgttgcaat cagaacggcc aagaaatttc ttttcatttt
     5221 gactcctcag tagaaagttc tgcgaaatga ctggaggctg ccggacccgg cctcactttc
     5281 ctcagtaggc cacagtgtat tcagcaggga gtggattcag gaggagggta ctcccccaat
     5341 cggtcacgct ggcctttcgg acaggttccc tcgatggcgc attagttctt ttctggtatt
     5401 cggaaagcag tacggttcga atgccaacgc caatccggcg gacgttggtc aggatctaat
     5461 tcggagaaaa aggccgtaga ccgtgaatcc caggccaccc aggaaaagca gcggcgtgag
     5521 gtattgcgtc accagcggca tcacgggcgc gggcaaggga atctgaaaaa aaggagcgcc
     5581 gtagccgccg gcgaaatagt ggatcacggc tccgagaagc atgtagagca acccgtgccg
     5641 gatctgacgg cgtccattgg aaccggtacg gcgagacact tgcacctgcg ggaccgcctc
     5701 gcggcaaaag gggcactgcg tcagatgcgc cgccatgtgc ttgccgcaaa atacgcagcg
     5761 gcgcgtgtcc ccggtcggtt ttccaaaagt gctcatacag ct
//
LOCUS       DASYVO010000363         8055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_38576, whole genome shotgun sequence.
ACCESSION   DASYVO010000363 DASYVO010000000
VERSION     DASYVO010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8055
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38576"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VGR03_12225"
     CDS             complement(<1..182)
                     /locus_tag="VGR03_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995089.1"
                     /translation="MDRRKLGMAMKFFGAMMLMAAAPSLQAPLHAQDVPAQREAQKKG
                     TGQVPAGVTLSPQMPAA"
     gene            complement(236..1615)
                     /locus_tag="VGR03_12230"
     CDS             complement(236..1615)
                     /locus_tag="VGR03_12230"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV1995090.1"
                     /translation="MRIRCITNCLIALLALSATARLAAAPGDTAGPEGSGRPRPVVIG
                     SHTLGNGLRLLMVEDHTAPVINLQVWYHVGSKDERAGRTGFAHLFEHLMFKGSAHVGA
                     DEHSRLIEAIGGFDNASTNDDTTIYWETFPSNYLERMLWLEADRLGSLNVDEANFKSE
                     REVVKEERRAHYENPPYGLIVEDLYAAAFSVHPYKHTTIGSMEDLNKATLEDVCDFFH
                     TFYRPDNATVVIAGDFTAAQAVSWSEKYFGGIPRPAPPLPRVTVQEPPQTAEHRVTKS
                     YGANSPLPAVVEGYKMPAAYTPDSYPLNLASNILSGGESSRLYRKLVYEDRIAVQSAG
                     FGSFTEHPNLFIAYAVMNQGKTPAEGEKAMEAILEQMRSSPVDAKELDKAKNQQIGGF
                     ILGRETVQEIANALGHDAVIGGDAELLNTNLDRYLRVTPADVQRVAREYFTAARRTIL
                     IVETPKGAK"
     gene            complement(1955..2740)
                     /locus_tag="VGR03_12235"
     CDS             complement(1955..2740)
                     /locus_tag="VGR03_12235"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEV1995091.1"
                     /translation="MKMRSWWVIGLAMALVVAIAFPPEVTSQALRSAGKISRMIPTVN
                     LQRASKKMDAATQTPVQWGDTLLSQQGGRARIALEDGSILNLGSESSLTILSHDAATQ
                     KTQMQLAYGRVRSNVVRLARAGSSFEVRTPAAVAGVVGTDFYIAHENGVTTVIVFEGV
                     VRVCALAGNCVDVHAGEMTTVREGQKPDSPRKASPADVMEAGHLTDVPGAEARLVEGG
                     VHNRWVIFGLILSAVLPAVLIPTLTNRGGSSPSSTCTGPYCGI"
     gene            3024..3344
                     /locus_tag="VGR03_12240"
     CDS             3024..3344
                     /locus_tag="VGR03_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995092.1"
                     /translation="MPLACSAALLFAMALLFAAEPAGAQLPPSKGAPKVGEKMPDFTL
                     PDTNNKPVKLSALLAPAAEEKPGAGEKPAAGEKPGAGEKPATEEKKDQAGSWVVLIFY
                     RGYW"
     gene            3357..3704
                     /locus_tag="VGR03_12245"
                     /pseudo
     CDS             3357..3704
                     /locus_tag="VGR03_12245"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            3960..4289
                     /locus_tag="VGR03_12250"
     CDS             3960..4289
                     /locus_tag="VGR03_12250"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEV1995093.1"
                     /translation="MSDLATKACVPCRGGVPPLKGEELASLQKQVDGWNVMEEHHITR
                     TLKFPDFRQALKFVNQVGNLAEEQGHHPDIFLAWGRVEITIWTHKIDGLTESDFILAA
                     KIDQLYK"
     gene            complement(5788..6063)
                     /locus_tag="VGR03_12255"
     CDS             complement(5788..6063)
                     /locus_tag="VGR03_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995094.1"
                     /translation="MRISTNRHKLSTTVSRDTQSYLETLVESGRAASIAEAVDLAVAR
                     ARRTESRAMLERDTAAYFDGLTARSAAEESRLGRTLGRVVDEVDFDI"
     gene            complement(6746..7507)
                     /locus_tag="VGR03_12260"
     CDS             complement(6746..7507)
                     /locus_tag="VGR03_12260"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEV1995095.1"
                     /translation="MPAPAFDVDALRQLRDSELEEARNLQRCLVPNSALRGSSIEFVS
                     RFQPVSEVGGDFLDYFFLSDRRIGIYLGDVVGKGLPAAMYAALTVGTLRGIHKTGSPP
                     TTVLSLLNRRLRTRTVPGRYSAVQYAVYDPATRELWYANAGLPRPIHISADNCQEVGE
                     GGLPSGLFDDARYDQWNITLKPGDAVLFATDGLMEAHNPDREEFGSERIMDVCRRNLA
                     GPPDLLLDRLFEAVLEFTAGQPQQDDITAALMKTS"
     gene            complement(7522..7743)
                     /locus_tag="VGR03_12265"
     CDS             complement(7522..7743)
                     /locus_tag="VGR03_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995096.1"
                     /translation="MFIPPRWSFCFLLLLGGRARVPKSVADWSILARSARIGKHLHAK
                     QSGEKWLFAAPPDDMMRGMRASRSKIQQE"
     gene            7742..>8055
                     /locus_tag="VGR03_12270"
     CDS             7742..>8055
                     /locus_tag="VGR03_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995097.1"
                     /translation="MERTIAVFALILLAAGAAASQSEFPSIVNFLRVDKQICTGGQPS
                     LEDLARLKASGVKAIINLRRPAEFNAADEEARAKQLGLRYFLISVDSRDPKDVQAEEF
                     LKV"
BASE COUNT         1562 a         2530 c         2377 g         1586 t
ORIGIN      
        1 gccgcgggca tctgcggaga cagcgtgacg cctgcgggca cctggccggt gccttttttc
       61 tgcgcttcgc gttgtgccgg cacgtcctgc gcatgcaacg gggcctgcag ggatggcgcc
      121 gcggccatca gcatcatggc gccgaaaaac ttcatggcca tcccgagctt tcttcgatcc
      181 atcaaccggc tcctttaccc tgcgggaaaa attttgtgct gcgggcctcg cggcatcatt
      241 tggccccttt gggagtttcc acgatcagaa tcgtgcggcg cgccgcggta aagtattccc
      301 gcgccacgcg ttgcacgtcc gccggcgtca cgcgcagata tcggtccagg ttcgtattca
      361 gcagttccgc atcgccgccg atcaccgcgt cgtgacccag cgcgttggca atctcctgca
      421 ccgtctcccg gcccaggatg aagccgccga tctgctgatt cttggctttg tccagctcct
      481 tggcatccac gggcgagctt cgcatctgct cgagaatcgc ctccatcgct ttttcgcctt
      541 cggcgggcgt cttgccctgg ttcatcaccg cgtaggcgat gaacaggttg gggtgttcgg
      601 tgaaggaacc gaagcccgcg ctctgcacgg cgatgcgatc ttcgtagacc agtttgcgat
      661 agaggcggct gctctctccg ccggaaagaa tgttcgaggc caggttaagc gggtaggaat
      721 cgggcgtgta agcggcgggc attttatatc cctcgaccac cgccggcagc ggtgaattcg
      781 cgccgtacga ttttgtgacc cggtgttccg cggtctgcgg cggctcctgc acggtcaccc
      841 gcggcagcgg cggcgccggg cgcggaatcc ctccgaagta tttttcgctc caggagacgg
      901 cctgcgccgc ggtaaagtcg ccggcgatca ccaccgtcgc gttgtcgggg cgataaaacg
      961 tatgaaaaaa atcgcacaca tcctccagcg tcgctttgtt gaggtcttcc atgctgccga
     1021 tggtggtgtg cttgtagggg tgcacggaga acgccgccgc gtagagatcc tcgacgatca
     1081 gtccatacgg cggattctcg taatgcgcgc ggcgctcttc cttcaccacc tcgcgttccg
     1141 atttgaagtt ggcctcatcg acgttcaggc tgcccagccg gtcggcctcc agccacaaca
     1201 tgcgctcgag gtaattgctg ggaaatgtct cccagtagat cgtggtgtcg tcgttggtcg
     1261 aggcgttgtc gaagccgccg atcgcctcga tcaggcgcga gtgttcgtcc gcgcccacgt
     1321 gtgccgagcc cttgaacatc aggtgctcga acaggtgcgc gaagccggtc cgcccggcgc
     1381 gctcgtcctt ggaaccgacg tgataccaca cctgcagatt aatgaccggg gcggtgtgat
     1441 cctccaccat cagcaggcgc agcccgttgc ccagcgtgtg gctcccgatg accaccggcc
     1501 gcgggcgccc cgaaccttcg gggcccgctg tgtcgcccgg ggcggcggcg agacgcgcgg
     1561 ttgccgatag ggccagcagc gcaatcagac aatttgtgat gcaacgtatg cgcatttctc
     1621 aaacctcgct ttcggatccg gttagcagga gtttctacgg caaatgagga aggctgcggt
     1681 cccacccgcg caccggctac tatcctaggc ggtgcgaaat cgggagtcaa attgcgcgcc
     1741 cgcggctccg gagaaatgag agacgagccg ggcggtcccc gcgttccgat attctgtggc
     1801 gctccgaaat tctgtggcgc atcgttcgcg ccgatccctt ccgccacgaa gaatgaccgt
     1861 cgcggcccgg cggcagaaga aacaaaaggc gggagaggag gcgcctgctc ctctcccgcc
     1921 ggagttcttc cttcgaattg gagatgtctg cccgctaaat gccgcaataa gggccggtgc
     1981 aagtcgagct cggagaggaa ccgccgcgat tggtgagtgt gggaatcagc acggccggga
     2041 gcacggcaga caatatcagg ccgaaaatga cccagcggtt gtgcacgcca ccctcgacga
     2101 gcctggcttc cgcgcccggc acgtcggtga gatggccggc ctccatcacg tcggccggcg
     2161 aggccttcct cggcgagtcc ggcttctgcc cctcacgcac cgtggtcatc tcgccggcat
     2221 gcacgtcgac gcagttgccg gccagggcgc agacgcggac cacgccctca aacacgatca
     2281 ccgtggtgac gccgttctcg tgggcgatat agaaatcggt gcccaccacg ccggccaccg
     2341 cggcgggcgt gcgcacctcg aagctgctcc ccgcccgcgc gaggcgcacg acgttcgagc
     2401 gcacgcgccc gtaggccagt tgcatctgag ttttctgggt cgcggcgtcg tgactgagaa
     2461 tcgtgaggct ggattcggag cccaggttga ggatcgagcc gtcctccagg gcgatccgcg
     2521 cgcgcccgcc ctgctggctc agcagcgtgt cgccccactg aaccggcgtc tgggttgccg
     2581 cgtccatttt tttggaggcg cgctgcaggt tcaccgtggg aatcatgcgc gagatctttc
     2641 ctgcagaacg cagggcctgc gaggttactt cgggaggaaa ggcaatcgcg accaccagtg
     2701 ccatcgccag gccgattacc caccaagatc gcattttcat ggctttactc cctcaacttc
     2761 gggagccgcc ccccagcggc gactgacccc gcacaagatt tgatggcaag gttaccgcgc
     2821 aacgggcggc gtcgcaacta cttcgttacc gacatgccgg atgggcgcat cccggccccg
     2881 gccaccccgc ctctcgccgc aaccattcaa cgtgccccgc aatcttcaat acctttgcat
     2941 cccgctgcgc tttcgtgcat ctatactccc accagccggg cagaagcagt gtgaagatgt
     3001 ccaagcaagt gttgagaatt cggatgccgc tcgcgtgttc cgcggccctt ttgttcgcta
     3061 tggccttgct cttcgcggcc gagccggcgg gcgctcagct tccgccgtcg aagggggcgc
     3121 ccaaggtcgg cgagaagatg cccgacttca ccttgccgga taccaacaac aaaccggtaa
     3181 agttgtccgc gctgctggca cctgcggcgg aagaaaagcc gggcgcgggg gagaaaccgg
     3241 cggcagggga aaagccgggc gcgggggaaa aaccggcgac ggaagaaaaa aaagaccagg
     3301 ccgggtcctg ggtcgtgctg atcttctacc gaggctactg gtgaccctac tgcaactccg
     3361 agttgcggag ttttcaagaa agcctcggca aattcaccgc acgcaacgtg cgcatcgtgg
     3421 ccatcagcgt ggacccgccc gatgtcagca agaatctcgc cgagaagcag ggctacacct
     3481 tccccatcct cgccgatgaa aaactcgaac tcatcaaacg tcttgacctg ctgcactccg
     3541 gcggcttccg cgggcaggac atctcccgcc ccgccgagtt cctggtcgac cccaaaggcg
     3601 tcgtgcgctg gttgaacctc accgaagatt acaaagtgcg cgccaaggcc gagcaggtgc
     3661 tcaaggtgct cgatgatctt ggccaggggc gcgagaagcc gtgacggtgt catccttcgt
     3721 ccgcctgcgg cggacgaagg atctctcttc atctccaagc gatttctggg ccaccctaaa
     3781 cgacgtcatt ccccgcgaaa agaaggatcc ctcttcgctt cccctcgcac cctttccgcg
     3841 gacggcgagc atccggcacc ccaatcttgt tcggtcacgc agggtgagcc gcccgctttt
     3901 gcgccgtcgc aaggtcacac ccgagattca ggcacgccga tgctagaata gtggttgtga
     3961 tgtcagacct cgcaaccaaa gcctgcgtcc catgccgcgg cggtgtacca ccgctaaagg
     4021 gtgaggaact cgcttctttg caaaagcagg tggacggatg gaatgtgatg gaggagcacc
     4081 acattacaag aaccttgaag tttccggact ttcgccaggc tctcaaattt gtcaaccagg
     4141 ttgggaatct cgccgaagag caaggtcacc atccggacat ctttctggct tggggaagag
     4201 ttgagatcac catctggacg cacaaaatcg atggtttgac agagagcgat ttcatcctgg
     4261 ctgcgaagat cgaccagctc tacaaatagt attggaatct acagagacgc tggcggccag
     4321 ggcagcttcc aagtcggtca aactgcaaag gcccgctcgg gctaccgtgt gcagtcactc
     4381 cgcaagggac atggccgcca gcgcctttcg gggaaaactg acaaatcata gctcagccgg
     4441 tgggagctag tccatcgggg aaatggggcg tgcccgagcc tttacaacgc gaaggcctgg
     4501 cagcattcct actcgcccgg aagcagccga cgccgttatc aaatcagacc aactggattt
     4561 taggccagag ccctcttgcg cctacgatct cgcttaaacc cgaatcgatc gcgctcaccc
     4621 gagaacactc catctggatc atagccgaag cgtctgaacg cctcggcccg caagaaccac
     4681 gcaaaaaaag ggaactcatc gctcatagca tcagccactg gatgcgtggc taccagtctc
     4741 gcgctttgcg tgaaatggtt accatccttc gccgcgtgga tcgtagttga ccgtcctaca
     4801 tacggaaact tgccggaata caaatggtga atcgaccaag cgttggaact tccgaaccgc
     4861 ccgggtctct cgccgccggc catcagaaac gccgcgatcg caggcccgtt cgctcgcttg
     4921 tcgaggtaaa tgtcccgacg cttggccaat cgacgaactg cctcggggta cttatgatat
     4981 ctctgcttct cacccggcgg tgccacacgg tcgcagtccc gccaaagaag aagtgctcga
     5041 cggggccact tcacgaaatc aggcgggtgg cccacccgca aggagtggta tcctcatggg
     5101 cggtggcgca ggcgggtggc gcagccttct ggacttcgtt ttgcggcgcg ggtgccccat
     5161 tccttccggg ctttgccgga agggtggggc gttcggtccc gctcctgatc cccaaaggcc
     5221 tgatgccgaa aggcccgatc tccgaagaat cgatacgcta ctccgcaaaa ggacatccgc
     5281 cgcaaccgcc tacggcctga ggatcttggg gcagacagct tggtctttgg gcaagagcca
     5341 gttcaattgt tttgttggtc tttccaagag ttcaccgcca ctaaaactag tcggccttac
     5401 taccgcttgc ctggcaacgg ttcgtaaggt gcattagggt caaaactacg gcgaataatc
     5461 tcgcaaacct gtctcatgcg cgcatcggaa agggggcgag gctgcctttc tctcaaccgg
     5521 gctttgggaa gtgtcgtcac gaaatggccc ttgaggtacg acgtcgcagg caacccggtt
     5581 tcccctggtt ccagtgggag aatcgttggt ccgacggctc ccgtggaact gaatggcacc
     5641 atcaagactg tatcgactcg gttgctttga ttccgtgaat cgagggatac aacaaggacc
     5701 cagtgacgac gggatggctc gccaagttcc gcggtccaaa tctctcctcg ccgcggcgat
     5761 ggcccaactc gtaagtccgc aggtggtcta gatgtcaaag tctacctcgt ctacgactcg
     5821 gcccagcgtt ctcccgagac gcgattcctc tgcagcagat cttgccgtaa ggccgtcgaa
     5881 gtaagcggct gtgtcccgtt ccagcatggc ccggctttcc gttcgccggg cccgcgccac
     5941 cgccaagtcc actgcttccg caatactggc agcgcgccct gattcaacca gggtttcgag
     6001 ataggattgg gtatcacgag aaacggtcgt ggacagcttg tgtcggttgg tagagatacg
     6061 catttgaagt ctccggggtc gccgcctctt gttccaagct cctacacaat cgcatatgtt
     6121 tatccccttg tcaaggagat attctggagg atgaaacagg tgacacgtgg gtgaccaatc
     6181 ctttcccgct gctggcgggt ggcgcagcct tccgaacttg gttttgccgc gcgggtgccc
     6241 cattccttcc gggctttgcc ggaagggtgg ggcctccggt gccgcttctg atctctaaag
     6301 gcccaacgcc cataggattg aaacgctact ctgggaaacg acatccgccg caagcgccta
     6361 cgatctgagg attttcgtgc agatggccct ttccccttcc ccctcgcccc tggcccctag
     6421 cccctggccc ctagcccctt gcacctcccc cctcccccac tttcctattg caatatgata
     6481 actcatatgg tactatactt cgcgtgagcg tcacgcctga cactcgatac tgtcccccga
     6541 ccactagcca ctgctcactg tccacaagtc actcaaatct cgctctatct cctttagaat
     6601 ccactcttac gcaatttacg cctctaaccc atttagaatc cactcttacg aatccaggct
     6661 ttttaagtcc tttggaatcc actcttacaa acaaaggggg gaggggggag gtaacttacg
     6721 ggttaacagc tctaagccga aaactctagg aggtcttcat cagcgctgca gtgatgtcgt
     6781 cctgctgagg ctggcccgcc gtgaattcca gcacggcctc aaagaggcgg tcgagcagca
     6841 ggtccggcgg accggccagg tttcgccggc agacatccat gattcgctcg gagccaaact
     6901 cttcgcggtc gggattgtgc gcttccatca gtccatcggt ggcgaacagt acggcgtcgc
     6961 cgggcttgag cgtgatattc cactgatcgt agcgcgcgtc gtcgaacagg cccgagggca
     7021 ggccgccttc gccgacctcc tggcaattgt ctgcggagat gtggatcggg cgcggcaagc
     7081 cggcgttcgc gtaccacagc tcgcgggtgg cgggatcata gacggcgtat tgaacggcgc
     7141 tgtagcgtcc cggcacggtg cgcgtgcgca ggcggcgatt gagcagcgaa agaacggttg
     7201 tgggaggcga gcccgtctta tggatgccgc gcagcgtgcc cacggtcagc gcggcgtaca
     7261 tcgccgcggg cagccctttg cccaccacgt cgcccaaata aattccgatg cgccggtcgg
     7321 agaggaaaaa atagtccaga aaatctccgc cgacctcgct gaccggctga aagcggctga
     7381 caaactcaat cgagctgccg cgcagtgcgc tgttgggaac caggcagcgc tgcaggttgc
     7441 gcgcctcttc cagttcggaa tcgcgcaact ggcgaagcgc gtctacatcg aatgcggggg
     7501 cgggcacgac caatcctcga gtcactcctg ctggattttg ctgcgtgatg cgcgcatccc
     7561 ccgcatcata tcatccggcg gcgcggcgaa gagccatttt tccccggatt gcttggcgtg
     7621 caaatgcttg ccgatgcgcg ccgatcgcgc cagaatggac caatcagcga cacttttcgg
     7681 gacgcgggca cgtcctccaa gaagcagcaa gaagcagaat gaccagcggg ggggaatgaa
     7741 catggaacga acgattgcag ttttcgcact gattcttctc gccgccggcg ccgccgcttc
     7801 ccaatcggaa tttccttcca tcgtgaactt tctgcgcgtg gacaaacaga tctgcaccgg
     7861 gggacagccg tcgctcgaag accttgcccg cctgaaggcc agcggggtca aagccatcat
     7921 caatttgcgc cggccggccg agttcaatgc cgcagatgag gaagcgcggg cgaagcaact
     7981 gggactgcgg tatttcctca tctcggtgga ttcccgcgac cccaaggatg tgcaggcgga
     8041 agaattcctg aaggt
//
LOCUS       DASYVO010000364         3407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_146712, whole genome shotgun sequence.
ACCESSION   DASYVO010000364 DASYVO010000000
VERSION     DASYVO010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146712"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VGR03_12275"
     CDS             complement(<1..421)
                     /locus_tag="VGR03_12275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1995098.1"
                     /translation="MAGGHAIPFVPRYDLPMANGVKRIGLISDTHGLLRKEAIEALRG
                     SEMIIHAGDVGKLEILEALRKIAPVVAVRGNVDTEPWAQALPETAVAEAGSVLIYILH
                     DVNALDLNPAASGFHIVVSGHSHKPGKTERDGVLYINP"
     gene            494..829
                     /locus_tag="VGR03_12280"
     CDS             494..829
                     /locus_tag="VGR03_12280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1995099.1"
                     /translation="MADKPSGLVQGTLDMLILKTLELEAMHGYGIAMRIEQTSKGVFQ
                     VNPGSLFPAFRRLERAGWLKSEWRDTENNRRAKYYLLTALGRKQLKGETREWARQAAA
                     IARILEAES"
     gene            840..>3407
                     /locus_tag="VGR03_12285"
     CDS             840..>3407
                     /locus_tag="VGR03_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1995100.1"
                     /translation="MSLLRNLAGGLRGLFRKEQVEREMDEELRGYLDAAVNEKIRRGM
                     SREEALRAARIEMGGAESVKEQVRAVSWESLVGTLWQDLKFGLRLLRFNPAFAAAAVL
                     SLALGIGANTAIFQLLDAIRMRTLPVKNPQELAEVRIAERNGATGAFVTRYPKLTSPQ
                     WEQIRAQQRGFSGLFAWGPTTFNISPGGEVHNVQGLWVSGEFFDVLGIEPERGRLLSP
                     PDDRPGCASPGVVISHSFWQREFGGDASALGRKITVEKHPFEVIGVTPASFYGVEVGR
                     NFDVALPLCVEPIIRSEFSVLNRRDGWWLTVMGRLKPGWTPEQVSVQARTISPGVFEA
                     TLPAEFDAGDAKHYLGYKLGVFPADTGVSELRSTYENPLWLMLGLAALVLLIASANLA
                     NLMLARASTREREMAMRMAVGATRGRLIRQLLAESLLLGLSGAVFGALLANGLTQLLV
                     ASISTSNQPLFVDLALDWRMLGFTTAITLLTCALFGLTPALRATQVAPGAILKSAGRN
                     ATNGPVRFGLRRVLVVSQIAMSLMLVVGALLFGRSLQKLATLDAGFTQDGVLEMDLDL
                     TALHLPSEGRAEFKRQVLDQVRAIPGVESAAEAGIVPLSGDGMNRQVLVNHAGQIVEG
                     KSEMNEISPKYFATWRTPILAGRDFDEGDTLRSPLVALVNETFAKKYLGGVNPVGATF
                     RVKSESKETGPYQIVGLVKDAKLYDLREDFLPTMFMPVAQKGHPEQEETILIRSEASL
                     FGLISSLKSTVQQFNRGIDMNFTPYRKMVETSLLRDRLMARLSGFFGILAVLLATVGL
                     YGVIAYMVERRTSEIGIRMALGADRGSIVRLVLREALVLLGLGLLIGTGLSLAGSRAA
                     "
BASE COUNT          659 a          987 c         1051 g          710 t
ORIGIN      
        1 ccgggttgat gtagagcacg ccatcgcgct ccgtttttcc cggtttgtga gaatggccgc
       61 tgacgacgat atgaaaaccg gatgcggcag gattcagatc cagagcgttc acatcgtgca
      121 ggatgtagat caggaccgag cctgcttccg ccacggccgt ctccggcaac gcctgcgccc
      181 atggctcggt atccacattt ccgcgcaccg ctacaactgg cgcaatcttt ctcaatgctt
      241 ccaaaatctc caacttcccc acatctcctg cgtgaatgat catctccgac ccgcgcaggg
      301 cttctattgc ttccttgcgc agcaagccgt gggtatccga aatcagaccg atccttttca
      361 ctccgtttgc catgggcaga tcgtaccgcg gaacgaatgg aatcgcgtgg cccccggcca
      421 aacgatcctc tcctcttgac aatctagggt aacgatgcca tcttcctctt gacagtcgag
      481 aggagcgcaa ttcatggcgg acaaaccgag cggcctggtc cagggcacgc tggatatgct
      541 gatcctgaaa acactggagc tggaagcaat gcacggctac gggatagcga tgcgcatcga
      601 gcagaccagc aagggcgtct tccaggtgaa tccgggttcg ctgtttccgg cgttccgcag
      661 gctggagcgg gccggctggc tcaagagcga atggcgggac acggagaaca accggcgcgc
      721 gaagtattac ctgctgacgg cgctggggcg gaaacaactc aagggcgaga cgcgtgaatg
      781 ggccaggcaa gcggccgcca ttgcgcgaat tctcgaggcg gaatcgtagg aggctgacga
      841 tgtcactgct gcgaaatctg gcaggcggtt tgcgcggatt gttccgcaag gaacaggtcg
      901 agcgcgaaat ggatgaggaa ctgcgcgggt atctggacgc cgccgtgaac gaaaaaatcc
      961 gccgcgggat gagccgcgaa gaagcgctgc gggccgcgcg catcgagatg ggcggcgcgg
     1021 aaagcgtgaa agaacaggtc cgcgccgtga gctgggagtc attggtcgga acgctctggc
     1081 aggatctgaa gttcggtttg cgtctcctcc ggttcaaccc tgctttcgcg gcagcggccg
     1141 tattgtcgct ggcactcggt atcggcgcca acaccgcaat tttccaactg ctagacgcca
     1201 tccgcatgcg cacgctgccg gtgaaaaatc cgcaagaact cgccgaggtc cggatcgcgg
     1261 aacgcaacgg cgcaaccgga gcgttcgtca cgcgctatcc gaagctgaca agtccgcaat
     1321 gggaacagat tcgcgcgcag cagcggggtt tttccggctt gtttgcctgg ggaccgacca
     1381 ctttcaatat ttctcccggc ggcgaagttc acaacgtgca gggcctgtgg gtcagcggcg
     1441 aattctttga cgtactggga atcgagccgg agagaggacg tctgctctcg ccacccgatg
     1501 accgtccggg ctgcgcatcg cccggagttg tgatcagcca ctcgttctgg cagcgcgaat
     1561 ttggaggcga tgcatccgcg cttgggcgca aaataaccgt cgaaaagcat cccttcgaag
     1621 tgattggagt aacgccggcc agtttttacg gcgtcgaagt cgggcgcaat ttcgatgtcg
     1681 ctttgccgct ctgcgtcgaa cccatcatcc gcagcgagtt cagcgtacta aatcgccggg
     1741 acggctggtg gctcacggtg atgggccggc tgaagcccgg ctggacaccc gagcaggtat
     1801 ctgtgcaagc gcgcacgatt tcaccgggag tctttgaagc cacgttgccc gctgaattcg
     1861 atgccggcga cgccaaacac tacctcggct acaagcttgg cgtgttcccg gccgatacgg
     1921 gcgtttctga attacgctcg acttacgaaa atcccttgtg gctgatgctc ggactcgcgg
     1981 cactggtgct gttgattgct tcggcgaatc tcgccaatct gatgctcgcg cgcgccagca
     2041 cccgcgagcg cgaaatggcc atgcgcatgg cggttggcgc aacgcggggc cggctgattc
     2101 gtcaactgct cgcggaaagt ttgctgctcg gtctttcggg tgctgtcttt ggcgccctgc
     2161 ttgcgaatgg cttgacgcaa ttgctggtcg cttcgatcag cacctcaaat caaccgctgt
     2221 ttgtggacct cgcattggat tggcgcatgc tcggtttcac gaccgcgatc acgttgctta
     2281 cctgcgctct tttcgggctg acgcccgcgc tgcgcgcgac ccaagtagct ccgggcgcga
     2341 tcctgaagtc cgcgggaaga aacgcgacga acggtcccgt acggttcggc ctgcggcgcg
     2401 ttctggtcgt ttcgcagatc gccatgtctc taatgttagt ggtcggggcg ctgctctttg
     2461 ggcgcagcct gcagaagctg gcgactctcg atgctggctt cacgcaagac ggcgtgctgg
     2521 agatggatct ggatttgacc gcgctgcacc ttccttccga agggcgcgcg gagttcaagc
     2581 ggcaggtgct cgatcaggtt cgcgctattc ccggcgtcga atctgctgcc gaggccggca
     2641 tcgttcctct cagcggcgat ggtatgaacc gccaggtgct cgtgaatcac gctggtcaaa
     2701 tcgtcgaagg caagagcgag atgaatgaaa tcagtccgaa gtattttgct acctggcgca
     2761 ctccaattct ggcgggccgc gatttcgacg agggcgatac cttgcgctca cccctcgtcg
     2821 ccctggtcaa tgaaaccttt gccaagaaat acctcggtgg agttaatcct gtcggcgcca
     2881 ccttccgggt aaagtcggag agcaaagaga cgggccccta ccagattgtc ggcctcgtga
     2941 aagacgccaa actctacgat ctgcgcgaag actttttgcc gacgatgttc atgcccgtgg
     3001 cacagaaagg acacccggag caggaagaaa ccatcctgat tcgttcggaa gcctctctct
     3061 ttggcctcat ctcttcgttg aaaagcactg tgcagcagtt caaccgcggc attgacatga
     3121 acttcacgcc atatcgcaag atggtcgaaa cttctttgct gcgcgaccgg ctgatggctc
     3181 gtctctccgg attttttggc attctggccg tgctcctggc caccgtaggt ttgtacgggg
     3241 tgatcgccta catggtggaa cggcggacca gcgaaatcgg gattcgcatg gccctgggag
     3301 cggatcgagg aagtatcgtg cggctggtgc tgcgggaagc tttggttctg ctcggcttgg
     3361 gtctgctgat cggaacggga ttgtccctgg caggcagcag ggcggcg
//
LOCUS       DASYVO010000365         6651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_124103, whole genome shotgun sequence.
ACCESSION   DASYVO010000365 DASYVO010000000
VERSION     DASYVO010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124103"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="VGR03_12290"
     CDS             <1..1458
                     /locus_tag="VGR03_12290"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEV1995101.1"
                     /translation="EITDKQRLNIGAATFYSGHHTVYQHAHFEFGLENVSRQFVWSFL
                     HAHPFYNSEQQSQRYVRLDRAQAYVPPVDLYFNQQCREIYEQAIAHAWNYYRELSALL
                     IGDTRTILNDIWHVSSMSNPKRLQKIERSAEKRAIEIARYVLPVAAFTTMVHTISGIV
                     LHRLWRMSAASDTPSEARAVIGAMVAHVKEIDAQFFDRFGTEPLEELPEWADRSAVAH
                     EKDAAANSNAGEAFTREFDAKLNGKTSILVDYSPNAVRVIAESYRAVTGLTASQCPDA
                     EAIDRLLNPARNLYRLETLNVGVHAPTMRALQHANYTFAKKISHTADSQDQRHRMVPG
                     SRPLLTLADTREPDYITPMLLAANPRAKEVYDRAMHDAWTAKNELLDRGVPPEIALYL
                     LPNAKSIRLYESGSLLHLIHKWTMRTCFNAQEEIYQASMDEIAQLREVHPELACYIGP
                     PCHIRAGITTPICTEGSHFCGIKVWLDFPNIIRRI"
     gene            1507..2175
                     /locus_tag="VGR03_12295"
     CDS             1507..2175
                     /locus_tag="VGR03_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995102.1"
                     /translation="MLRTSARHIAPVLVAVLLLTPSSFAFDMPLSDQAVREAYFLGQR
                     RDETMATFLNKYTKFLPPPKSGPDIASVTFLTPFALLVQQSSQHTSGYSAQQAALDHR
                     DQPEFVRIVVQIQFTESYGPYIARPTGSRSGSPTGFAPRPYDFWKDFDVQVSNEDQKL
                     RPFSSSGQPSLSCSDEGGCILTGATLQFDFSAEDFASGSAIIDVVPPEGDPLSVDFDL
                     THLR"
     gene            2274..2936
                     /locus_tag="VGR03_12300"
     CDS             2274..2936
                     /locus_tag="VGR03_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995103.1"
                     /translation="MRHLFRLFTSLLVALCVSLPAPSIDAPLSDQAVREAYFLGQRHE
                     GSYTSLLGNYIKRLPAPKSGPHISSVTLLTPFIQLVANSDRFIGNYSAQQALLDHRGQ
                     EESVKITVEIYLTNSYGPLIANPANSQSRSSSAFMPRPYDFWRDFRVQVYDGNRILSP
                     SASGGHPLYRCGRTGQCRPMGAALELEFPANSFPSDTISVYVTPPEGDPVSVDFDLTR
                     LR"
     gene            complement(3578..4723)
                     /locus_tag="VGR03_12305"
     CDS             complement(3578..4723)
                     /locus_tag="VGR03_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV1995104.1"
                     /translation="MSEAVRTILQTNGEAARESSSEADGGFLWDFWYPAVRSAEIAGQ
                     KLTTAMLLEVPLVLGRTSEGKAFAMRDSCPHRGIPLSYGRLDGKVVECCYHGWRFDAC
                     SGQCVEIPSLSSQDKLKVERIFAGHYPCEERDGYVWVYMTSRGRIAADNSPGTRLPET
                     IPAAPALTVFSDKYKITHLSCELPSHIDHGIIGLMDPAHGPFVHQSWFWRKRGSIHEK
                     AKQFEPIPNGFRMSPHSPSTNSAPYQLLKKITGEPVTTTIDFVLPNIRTEEDRSGKMW
                     FSSRATVTPVRRDLCRIDFVAAWNLFFLPVSIFRMFGKVFLRQDQETMIQQAEGLKHN
                     PRLMLIDDADRPAKWYFALKANLIESRRTGAAMVHPMDGPVTLRWRS"
     gene            complement(4873..5355)
                     /locus_tag="VGR03_12310"
     CDS             complement(4873..5355)
                     /locus_tag="VGR03_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995105.1"
                     /translation="MTLREFFLENFAGDLEYHPRRAMLYLGIAVAALCFWIFSPPEAK
                     FSILPLVFVLGSLTLLLKGIFLLRKSSEGLGLSQQEHDHLSDPSNRKSLPSLPNQAAQ
                     IVQDFGVGSLLLWPILSEGKDFDQSWNNPPQLRVFIIGAILFFLGWIIRRLTSTPTAH
                     "
     gene            5578..>6651
                     /gene="prfA"
                     /locus_tag="VGR03_12315"
     CDS             5578..>6651
                     /gene="prfA"
                     /locus_tag="VGR03_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV1995106.1"
                     /translation="MAALTLSEKLDQLGIRYDELTQELSSAEIVSDSSRFQKVAKQQS
                     ELGEIVAKHREFKQIEKDLAGAHQMFLEAEDAEMKQMAQDEEKELTARKEVAERELKL
                     LLLPKDPLDEKSVIVEIRAGTGGDEAALFAAELFRMYSRYAETQGWKVEVLESSPSSL
                     GGLKEAVAAIQGNKVYSKLKYESGVHRVQRVPATETQGRIHTSAATVAVLPEADEIDI
                     KIEAKDLRIDTFCSSGPGGQSVNTTYSAVRITHIPSGLVVSQQDEKSQIKNRAKAMRV
                     LRARLYEQEQEKRQVALSAERRGQIKTGDRSEKIRTYNFPQNRVTDHRIGLTVHQLDA
                     VMEGKLAPFVEGLMAHFQAERLNE"
BASE COUNT         1416 a         2271 c         1585 g         1379 t
ORIGIN      
        1 gaaatcaccg acaagcagcg cctcaacatc ggtgccgcca ccttctacag cggccatcac
       61 accgtctacc agcacgcgca tttcgaattc ggcctggaaa acgtcagccg ccaattcgtc
      121 tggtccttcc tccacgcgca tcccttttac aattccgagc agcaaagcca gcgctacgtc
      181 cgcctcgatc gcgcgcaagc ctatgtccct cccgtcgatc tctacttcaa ccagcaatgc
      241 cgcgaaatct acgaacaagc catcgcccac gcctggaact actatcgcga gctttccgcc
      301 ctcctgatcg gcgacacgcg caccatcctt aatgacattt ggcacgtcag ctcgatgtcc
      361 aatcccaagc gcctccagaa aatcgaacgc tccgccgaaa agcgcgccat cgaaatcgcc
      421 cgctacgtct tgcccgtcgc cgccttcacc accatggtgc acaccatctc cggcatcgtc
      481 ctccatcgcc tgtggcgcat gagcgccgcc tcggacacgc ccagcgaagc ccgcgccgtc
      541 attggcgcga tggtcgcgca tgtaaaagaa atcgacgcgc aattcttcga tcgctttggt
      601 acggaacctc ttgaagaact ccccgagtgg gccgaccgct ctgcggtcgc gcatgaaaaa
      661 gacgccgctg cgaattcgaa tgccggagag gcgttcaccc gcgaattcga cgccaaactc
      721 aacggcaaga cttcaatcct cgtcgactat tcgccgaacg ccgtgcgcgt cattgccgaa
      781 tcctatcgcg ccgtcactgg cctcaccgca tcgcaatgcc ccgacgccga agccatcgac
      841 cgccttctga atcccgcgcg caacctctac cgcctcgaaa cgctaaacgt tggcgtccac
      901 gccccaacaa tgcgcgccct ccaacacgcc aactacactt tcgccaaaaa aatcagccac
      961 accgctgaca gccaggacca gcgtcatcgc atggtccccg gctcgcgccc tctcctcacc
     1021 cttgccgaca cccgcgagcc cgactacatc acgcccatgc ttctcgccgc caatccccgc
     1081 gccaaagaag tctacgaccg cgccatgcac gacgcctgga ccgcaaaaaa cgaactcctc
     1141 gaccgcggcg tgcccccgga aatcgcgctc tatcttctcc ccaacgccaa atccatccgc
     1201 ctctacgaat ccggctcgct cctccatctc atccacaaat ggaccatgcg cacttgcttc
     1261 aacgcccagg aagaaatcta ccaagcctcc atggatgaaa tcgcccagct ccgcgaagtc
     1321 cacccagaac tcgcttgcta catcggcccg ccatgccaca ttcgcgccgg catcaccacg
     1381 cccatctgca ccgaaggctc ccacttctgc ggcatcaaag tttggctcga tttccccaac
     1441 atcatccgcc gcatctgaaa ctctcccgcg cgttttgccg tcttatactc tagctggagg
     1501 cccaccatgc ttcgcacttc tgcccgccat atcgccccag tccttgttgc ggttttgctt
     1561 ttaactccat cctcgttcgc cttcgacatg cctctgtccg atcaagccgt ccgcgaagcc
     1621 tatttcctcg gccagcgccg tgacgaaacc atggccactt tcctcaacaa atacacaaaa
     1681 ttccttccgc cgcccaaatc cggtccggac atcgcctccg tcactttcct cactcccttc
     1741 gcccttctcg tccagcaatc cagccagcac acatccggtt acagcgccca gcaagccgcc
     1801 ctcgatcatc gcgatcagcc cgaattcgta agaatcgtgg tgcaaatcca gttcaccgaa
     1861 tcttatggcc cttatatcgc tcgccccacc ggttcccgct ccggttctcc caccggcttc
     1921 gccccgcgcc cgtatgactt ctggaaagat ttcgacgttc aggtctccaa tgaagaccag
     1981 aaactccggc ccttctcttc atccggccag cccagtttgt cctgctctga cgaaggcggc
     2041 tgcattctca ccggcgccac tctccagttc gatttctccg ccgaagactt tgcctccggc
     2101 tccgccatca ttgacgtcgt tcccccggag ggcgatcccc tctccgtcga cttcgacctc
     2161 acccacctcc gctgacttct tcgtccctcc gggaccggtc gagacgggaa taacatcttt
     2221 accgctgcgg caatcccgaa ttcagcgata tactttcttt tgggaacgtt ctaatgcgcc
     2281 acctctttcg ccttttcacc agcctcctcg tggctctttg tgtgtctctg cccgcacctt
     2341 ccatcgatgc tcctctctcc gatcaagccg tccgcgaagc ctatttcctc ggccagcgcc
     2401 atgaaggttc ttacacttct ctgttgggca attacatcaa gcgcctgcca gcccccaagt
     2461 ccggcccgca tatctcttct gtcactctcc tgacgccttt tattcagctc gtggcaaact
     2521 ccgaccgttt catcggcaac tacagcgcgc agcaagccct gctcgaccac cgcggccagg
     2581 aagagtccgt caagattact gttgaaatct atctgaccaa ttcctatggc cctctgatcg
     2641 ccaatccagc caactcacaa tcccgatctt cctctgcttt catgccgcgt ccctatgact
     2701 tctggagaga ctttcgcgtc caggtatatg acggtaatcg catcctttcg ccctccgctt
     2761 ctggcggtca tccgctctac cgttgcgggc gcactggcca atgccgtccc atgggcgctg
     2821 ccctcgaatt ggaattcccg gccaactcct tcccctccga taccatctcc gtctacgtca
     2881 ctccccccga aggtgacccc gtctccgtcg acttcgacct cacccgcctc cgctgacttt
     2941 ctcgattcgg ctcgcgcccg tgcgcagcac tcccgtcggg cccgcctggc gcctgccccg
     3001 cgcctcgcgg ggaggctggc cgttcctgcc tcggccccga ttcagccctg gatctcccac
     3061 aaaataaagc cgatcttcgg cttgactttg agttaatctg tgatacactt attcctgtac
     3121 atagctacgc attcccgaat tggaatttct atcagccctc acaaccctcg gcctcccttc
     3181 tcggactcaa ctcttttcgt ttgatgggtt tgcgaattgg tctgaagcaa tgtgaaattg
     3241 cggcccttta gaatcaatca attacaaatg cccccttttc taagtcactt gttttgatac
     3301 ccttacaaat gcccgggggg tagagggtac cccgccggga ttcctcgagt attgctttaa
     3361 cttgcgggac ccttgaaaag tgagtggcta ctcccgcaaa gagtgggacg gacaatggag
     3421 cgcgaatcac gcagcaacga acccggatgc tgcatggctg cccttcgcat ccgatagctt
     3481 tacttacaac ttaaaactaa gaactaaaaa ctctttaaga ccgccaccgc aacatcatcg
     3541 gtcccggtgg gacaggcatt cttgcctgtc cccactctca cgaccgccac cgcagcgtca
     3601 ccggcccatc catcggatgc accatcgccg cgcccgtccg ccgcgactcg atcaggttgg
     3661 ccttcagcgc gaaataccac ttcgccggcc ggtccgcgtc gtcgatcagc atcaaccgtg
     3721 gattgtgctt caatccttcc gcctgttgga tcatcgtctc ctgatcttgg cggagaaaga
     3781 ccttgccgaa catccggaaa atcgaaactg gcaggaaaaa cagattccac gccgccacga
     3841 aatcgatccg gcacaaatca cgccgcaccg gcgtcaccgt cgcccggctg gaaaaccaca
     3901 tcttcccgct gcgatcctct tccgtgcgga tattcggcag cacgaaatcg atcgttgtcg
     3961 tcaccggctc gccggtgatt ttcttcagca actgataggg cgcgctgttc gtgctcggcg
     4021 aatgcgggct catccggaaa ccgttcggaa tcggctcgaa ttgcttcgct ttctcgtgaa
     4081 tgcttccgcg cttccgccag aaccacgact ggtgcacgaa cggcccgtgt gccgggtcca
     4141 tcaatccaat aatcccgtga tcgatgtgcg aaggcagctc gcaggaaaga tgtgtgattt
     4201 tgtatttgtc gctgaaaacc gtcagcgcgg gcgccgccgg aatcgtctcg ggcagccgcg
     4261 tccccggact attgtcggcc gctatgcggc cgcgcgaggt catgtagacc caaacgtagc
     4321 cgtcgcgttc ttcgcaggga tagtgcccag caaagatgcg ctctactttc agcttgtcct
     4381 ggctggaaag cgacggaatc tcgacgcact gtccgctgca agcatcgaac cgccacccgt
     4441 gatagcagca ctccaccact ttcccgtcga gccgcccgta cgacagcggg atgccccggt
     4501 gtgggcaaga atcccgcatc gcaaacgctt tcccctccga cgtccgcccc agcaccagcg
     4561 gcacttccag caacatcgcc gtcgtcagct tctgcccggc aatctccgcg ctccgcaccg
     4621 ccggatacca aaaatcccac aaaaatcccc catccgcctc ggacgaggat tcccgcgccg
     4681 cctcgccgtt tgtttgcaag atagttcgta ccgcttcgct catagtcgaa tcgcagtcag
     4741 gttttgtagc ggccgccttc cgaggcgggc gcattcaact gagccgcatc gtagtatatc
     4801 gcctcgcgga ggagcaacca tgtcgaggtc gccttgctcg cagttcggac cgttcgcagg
     4861 atcttctctt agtcagtgcg cggttggagt agaagtaagg cggcggatga tccagcccag
     4921 gaagaatagg atggcgccaa tgataaagac gcgcagctgc ggcgggttat tccaagactg
     4981 gtcgaaatcc tttccctcac tgagaatggg ccaaagaagg agtgagccta cgccgaaatc
     5041 ctgaacaatc tgggcggctt ggttcgggag ggacgggaga ctcttgcgat tggaaggatc
     5101 agaaagatga tcatgttctt gctgagagag cccaagtccc tccgatgatt tccggagaag
     5161 gaaaatgcct ttcaacagaa gcgtgaggct gccgagaacg aagacaagag gaagaatact
     5221 gaatttggct tccggtggtg agaagatcca aaagcacagg gcagccacgg caatgcccag
     5281 ataaagcatc gcgcggcgag gatggtattc caggtcgccg gcaaagtttt ccaagaagaa
     5341 ttcgcggagt gtcacggtcc gcagtttacc tcaaataggg gcgcataacg tgttccgaga
     5401 aattggctca ttccaaactc cgatttgcgt gatttgggat tcggcgctat tcttagtgca
     5461 gagcaaatcg atgtacgcca ccttttatta gctgactctc cccaccgctc ccgcaacgga
     5521 gcgccgcgat tcttcccatt ttctattttc caatttcaat tttccaaagg attccccatg
     5581 gccgcattga cgttatctga aaagttggac cagctcggca ttcgctacga cgagctcacg
     5641 caggaacttt cctcggcgga gatcgtctcg gactcctcgc gcttccaaaa ggttgccaaa
     5701 cagcaatccg aactcggcga aatcgtcgcc aagcaccgcg aattcaaaca gattgaaaaa
     5761 gatctcgccg gcgcccacca gatgtttctc gaagcggaag acgccgaaat gaagcagatg
     5821 gcgcaggacg aagaaaagga actcaccgcg cgcaaggaag tggccgagcg cgagctaaaa
     5881 ctcctgcttc tcccgaaaga tcccctcgac gaaaagagcg tcatcgtgga aatccgcgct
     5941 ggcaccggcg gcgatgaagc cgctctcttt gccgccgaac ttttccgcat gtattcgcgc
     6001 tacgcggaaa cgcaaggctg gaaagtcgag gtcttggaga gttcaccttc ttcgctcggg
     6061 ggcttgaaag aagccgtcgc ggcgattcag ggtaacaaag tttattccaa actgaagtat
     6121 gaaagcggcg tgcaccgcgt ccagcgggtt cccgcgacgg aaacgcaggg ccgcattcat
     6181 acttcggctg cgacggtcgc ggtacttccc gaagccgacg aaatcgacat caagatcgaa
     6241 gcgaaagatt tgcgtatcga caccttctgc tcctccggcc ccggtggtca gtccgtgaac
     6301 acgacctact ccgccgtgcg catcacgcac atccccagcg gactggtcgt ttcccagcaa
     6361 gacgaaaaat cgcaaatcaa gaatcgcgcc aaagccatgc gcgttctccg cgcgcgcctg
     6421 tacgaacagg agcaagagaa gcggcaggtc gctctctccg ccgagcgccg cggtcagatc
     6481 aaaaccggag accgctccga aaaaatacgc acctacaatt tcccgcaaaa ccgcgtcacc
     6541 gatcaccgca tcgggctcac cgttcaccag ctcgacgccg tgatggaggg caagctggcc
     6601 cccttcgtcg aaggtctgat ggctcacttt caggccgagc ggctgaatga g
//
LOCUS       DASYVO010000366         6003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_25063, whole genome shotgun sequence.
ACCESSION   DASYVO010000366 DASYVO010000000
VERSION     DASYVO010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25063"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..2173
                     /gene="ftsH"
                     /locus_tag="VGR03_12320"
     CDS             224..2173
                     /gene="ftsH"
                     /locus_tag="VGR03_12320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV1995107.1"
                     /translation="MNSGSTLKTILFWLSIVLLGVMLWKLVSANGGQPREDEPSYSEF
                     MQKVDTGQVKEVTMYLSANSYELKGEYAQPANRKFHTTVFKEDAPGLAKTLREKAVQT
                     KVIEVHSGDWVLILLNAAPLILLVGFCLFLMRQMQSGGNKALSFGKSRARLLSAQQKK
                     ATFKDVAGTDEAKEELQEIIEFLKDPQKFQKLGGRIPKGVLLVGPPGTGKTLLARAIA
                     GEANVPFFSISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGAGL
                     GGGHDEREQTLNALLVEMDGFETNEGVILIAATNRPDVLDPALLRPGRFDRRVVVARP
                     DVKGREEILRVHTRKVPISDDVDLSIIARGTPGFSGADLANLVNEAALWAARQNRKGV
                     LMVDFEMSKDKVMMGVERRSMILSDEEKKNTAYHEAGHALVAEMTPGADPVHKVTIIP
                     RGMALGLTMQLPEDDKHTYTREYLEAMLAVLMGGRSAEEIFLGHITTGAGNDIERATD
                     IARNMVCEWGMSELGPLAYGKKEEAIFLGREIAQHRDYSEDTAIQIDKEVKRIVNGGY
                     ESAKNLLANNRETLERIAQALLEREVIDAIEVKLLMEGKPLPEKPRTPPTPPPQAAPS
                     SDPKVVRPELRPAPGFTRGENPAKA"
     gene            2476..3330
                     /locus_tag="VGR03_12325"
     CDS             2476..3330
                     /locus_tag="VGR03_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995108.1"
                     /translation="MVKNLSPENFRISQLGDSPSRLAKVSLDAGPKRVALILDVSSNV
                     TKKEWELETEMAASLVKHGRKDDRFQITLAGADVEVDEFAGPGVVGERLKKLASSRPL
                     SAGPGEKLYDGILAAAKRMDPAQFGDALFFFGHVEDSGSTTDPAQVRELLLRNRFRLY
                     GLSFTDPLRGKLPAGYNPNKPLPATIVPSKLESISSATGYFVSFHSLEALALKNQPAL
                     LKDFLAELYAGIIEPYRLRIPASGLQGPLDLKIELANAAERGIRPADVHYPQYVYACD
                     LPTPSVAP"
     gene            3378..3686
                     /locus_tag="VGR03_12330"
     CDS             3378..3686
                     /locus_tag="VGR03_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995109.1"
                     /translation="MAKQNSENLHVTCPCCQAKLTVDPVFGAVLSHEAAVKAGPNVDL
                     SDAQKILAEQNRQREDKFADSWFQETNKEDILTKKFEEAMKKAKDAPAGKPIRDFDLD
                     "
     gene            3796..4680
                     /gene="folP"
                     /locus_tag="VGR03_12335"
     CDS             3796..4680
                     /gene="folP"
                     /locus_tag="VGR03_12335"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV1995110.1"
                     /translation="MQKRKKFRLRLASQTLLLGERTLVMGVLNVTPDSFSDGGKFFDA
                     ESAIERALAMERAGADLLDIGGESTRPGSAGISAQEELARVLPVLQALRGRIKIPVSI
                     DTQKSEVAEAALDAGAQIINDISGLKSDPRIAEVAARRRVPLILMHMRGEPRTMQTAR
                     FARDVMKDVLQGLRKSVGAACKAGVGKSQIILDPGIGFGKSYAQNYELLQEIPQLAKL
                     GYPLLVGTSRKGFLGATLARDGKPAPAEERIWGTAATVTASILNGAHIVRVHDVAEMV
                     QVARVADCLLDPKQRPRN"
     gene            4832..>6003
                     /locus_tag="VGR03_12340"
     CDS             4832..>6003
                     /locus_tag="VGR03_12340"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1995111.1"
                     /translation="MRRRTIFFPFLLFCFAAYLLPARGQLPPAKKDPLSPAQSATGKA
                     GCSTTEASSCAEAAAKILPIVMGSSPMEENLRRLTDEIGGRVTGSPQMAKAVEWGVGA
                     FRAAAVDVHTEKYMLPVTWSEGDTRLELLGPVKFPVRLKSEGWSPATPAGGIEANVID
                     VGYGNEDDFAKAGGLVKGAILLVHSDIGSTWADLFNEYLRPPMIIEHAVKEGARAILW
                     MGARERLLLYRHTNSLAGEIDRIPQAVLAREDAMRLARTVEAYPGKVRAHFDMPNKIG
                     GAIEQENVVGEIRGYEKPDEVVILGAHLDSWELGTGALDNGCNAALVIEAARAIKATG
                     LLPRRTIRFVLFSGEEQGTVGSFEYVKVHRAELDKLRAMITYDAGIGRVTGYSLGGR"
BASE COUNT         1342 a         1636 c         1887 g         1138 t
ORIGIN      
        1 cggcaaagcg gcgggtagcc ctggagcctg attactttgg cttccgaacc gcatctaacc
       61 aattgtgggt tggctacggc cttgcagcgg ccaacgggac ggggcgcaat ctccggcagc
      121 tttcttccgg agcgaatgga acgcccagga aaagtgttaa ggggcggaag aaataagaaa
      181 gatttgattt tttccggcag gttgcaggat tagtgaggac aacgtgaatt ctggttcaac
      241 actgaaaaca attctcttct ggctttccat cgtgctcctg ggcgtgatgc tctggaagct
      301 ggtgtccgcg aacgggggac agccacgtga ggatgaaccc agttacagcg aattcatgca
      361 aaaggtcgac accggccagg taaaggaagt cacgatgtat ctttccgcaa acagctatga
      421 gctgaaaggg gaatacgctc agccggcaaa ccgtaaattc cacacgacgg tgttcaagga
      481 ggatgcgccg gggctggcca agaccctgcg cgaaaaggcc gtacaaacca aggtgataga
      541 agtgcacagc ggcgactggg tgttaatcct attaaacgcc gcgcctttga ttcttcttgt
      601 gggcttttgc ctgttcctca tgcggcagat gcagtcgggc ggaaacaagg cattgagttt
      661 cggcaagtcc cgcgcgcgat tgctctccgc gcagcagaag aaggctacgt tcaaggacgt
      721 cgccgggacg gacgaggcca aggaagaact gcaggaaatt attgaattcc tgaaagatcc
      781 acagaagttc cagaagctgg gcgggcgaat tccgaagggc gtgttgcttg tcggacctcc
      841 gggcacgggc aaaacgctgc tggcgcgcgc catcgccggt gaggccaacg ttcccttctt
      901 ctcgatttcc ggttcggact tcgtggagat gttcgtgggc gtgggcgcga gccgcgtgcg
      961 cgatctcttt gaacagggca agaagaatgc gccctgcatc atcttcattg atgaaattga
     1021 cgctgtcggc cggcaccgcg gtgcgggtct cggcggcgga cacgacgagc gcgagcaaac
     1081 gctgaacgcg ctgctcgtgg agatggatgg ttttgaaacg aacgaaggcg tgatcttgat
     1141 cgcggcgaca aaccggcccg acgttctgga tccggcgctt ttgcggcctg gccgcttcga
     1201 ccgccgcgtg gtagtggcgc gtcccgacgt gaagggccgc gaagagattt tgcgcgtaca
     1261 tacccgcaag gtgccgatca gcgatgacgt ggatttgtcc atcatcgctc gcggcactcc
     1321 gggcttctcc ggcgcggacc tcgcgaactt ggtgaacgaa gcggcgctgt gggccgcgcg
     1381 acagaaccgc aagggcgtcc tgatggtcga cttcgagatg tccaaagata aagtgatgat
     1441 gggcgtcgag cgaagatcga tgatcctatc ggacgaagag aaaaagaaca ccgcgtatca
     1501 cgaggccggc cacgctctgg ttgcagagat gacgccgggc gctgatccgg ttcacaaagt
     1561 gacgatcatt ccgcgcggca tggcactcgg cctcacgatg cagctgccgg aagacgacaa
     1621 gcacacctac acgcgcgaat atctcgaagc gatgctggcg gtgctgatgg gcgggcgttc
     1681 cgcggaagaa atttttctcg gccacatcac caccggcgcg ggcaacgaca tcgaacgcgc
     1741 cacggacatt gcgcgcaaca tggtctgcga atggggtatg agcgagctgg ggccgctggc
     1801 ctacggcaag aaggaggagg ccatcttcct gggccgcgaa atcgcgcagc accgcgacta
     1861 ttccgaagac accgccatcc agatcgacaa ggaagtgaag cgcatcgtca acggcggcta
     1921 cgaaagtgcc aagaatctcc ttgcgaacaa tcgggaaacg ctcgagcgca tcgcgcaggc
     1981 gctcctcgaa cgggaagtca tcgacgccat tgaagtgaag ttgctcatgg aaggcaagcc
     2041 gctgccggaa aagccgcgca cgcctcccac gccgccgccg caggctgcgc cgagcagcga
     2101 ccccaaggtg gtgcgtccgg agctgcggcc agcgccgggc tttacgcgcg gggaaaatcc
     2161 agccaaagcg taatcccgct tgaatcttcg aaaactcctg aagagctcct gcacattaag
     2221 gggctcttcg cttgcctaaa gacgtaaagt tgcctgcaaa tcattgctga tgaactcaag
     2281 ttgacgtgag tgactgtgat cgttagcatg tacgcattcg gaggcgtgat gaagagcgct
     2341 gcgcccttct tgttgtcctg cctgattttt ttcttctcct ttgcaaactc atcctttgcg
     2401 cagcagagcc cggaaggggc atgcgacgtt cctctggtcg ccactcgata tgactctgca
     2461 ttgagaagag tcgagatggt taaaaacctc agcccagaga attttcggat aagccagctc
     2521 ggagattcac cttccagact ggcgaaggtg tcgctcgatg ctggtccaaa gcgcgtggcg
     2581 ctgatcctgg acgtgagcag caatgtaacc aagaaagagt gggagctgga aacggagatg
     2641 gccgcaagcc ttgtgaaaca tggaaggaaa gacgaccgct tccaaatcac cttggccggc
     2701 gcggacgtcg aagtggatga attcgcgggg cccggcgtcg tgggagaacg gttgaagaag
     2761 ttggcgtctt cccgcccgct tagtgccgga ccgggcgaga aactttacga tggaatccta
     2821 gctgctgcca aacgcatgga tcctgcacaa ttcggcgatg cgcttttttt cttcggacac
     2881 gtcgaagatt ccggaagcac tactgatcca gcgcaagtac gggaactcct tctaagaaac
     2941 agattcagat tgtatggcct gagttttacc gaccccctgc gcggaaagtt gcctgcgggt
     3001 tacaacccca acaagccact gccagcgact atcgtgccct cgaaactgga aagtatcagc
     3061 tccgctaccg gctatttcgt ctcttttcac agccttgagg cgttggcgct caaaaatcag
     3121 cctgcccttc tgaaagactt cctcgccgaa ctctacgcgg gaattatcga accctacagg
     3181 cttagaatcc ccgcgtctgg gttacaaggc ccgttggatc tcaagatcga gttggcgaac
     3241 gctgcggaac gtgggattcg cccggctgac gtgcactatc cccagtatgt ttatgcgtgc
     3301 gatttgccca caccatccgt ggcgccttga tcgcgccata gctcccaaac agccggcaag
     3361 aagcgtagaa tacggccatg gcgaagcaga acagcgagaa cctccacgtc acgtgcccgt
     3421 gctgccaggc caagctgacg gttgatcccg ttttcggcgc cgtgctttcg cacgaagcag
     3481 cggtcaaggc tggcccgaac gtggacctta gcgacgctca aaagatcctt gccgaacaaa
     3541 atcgccagcg cgaagacaag tttgcggatt cctggttcca ggagaccaac aaggaagaca
     3601 tcctgacgaa gaaatttgaa gaggcgatga agaaggccaa ggacgcgccg gcgggcaagc
     3661 ccatccgcga ttttgatctg gattaggtgg gacaggcatt cctgcctgtc cttgttcgtg
     3721 taatcgcagg agtgaaaatg acagactaga atgtctgtcc taccgtttac attacacttg
     3781 ctgccaaccg atagaatgca aaaacgaaag aaatttcggc tgaggctagc ctcccagacg
     3841 ctacttctcg gcgagcgcac gctcgtgatg ggcgtgctga atgtcacgcc ggacagcttc
     3901 tccgatggcg ggaagttttt tgacgcggag agtgccatcg agcgggcgct ggcgatggaa
     3961 cgcgccggag cggacttact tgatatcggc ggcgagtcca cgcggccggg ttctgcgggc
     4021 atttccgccc aggaagagtt ggcccgcgtg ttgccggtgt tgcaggcact gcgggggcgg
     4081 ataaaaattc cggtttccat cgacacgcaa aagtcggagg tcgcggaagc cgctctcgat
     4141 gctggcgcgc agattatcaa cgacatatcc gggttgaaga gcgacccgcg tatcgccgaa
     4201 gtcgccgcgc ggcgccgtgt gccgctcatc ctgatgcaca tgcgcggaga gccgcggacg
     4261 atgcagacgg caagatttgc gcgggacgtc atgaaagatg tattgcaggg gctgcgcaaa
     4321 tccgtggggg ccgcgtgcaa ggccggcgtc gggaaatctc agatcatcct cgatccgggg
     4381 atcggctttg gcaaaagcta cgcgcaaaat tacgaattgc tgcaagagat cccgcaactg
     4441 gcgaagctgg gctatccgct tttggtgggc acttcgcgga aggggttcct cggagcgaca
     4501 ttggcccgag acggcaaacc cgcgccggcg gaagagcgca tctggggcac cgccgccacg
     4561 gttaccgcga gcatcttgaa tggtgcacac atcgtccgcg ttcacgacgt cgccgagatg
     4621 gtccaggtcg cgcgcgtcgc cgattgcctc ctcgacccga aacaaaggcc aagaaattaa
     4681 acgcagaaac accccgaccg ggtcggggta aacgccggac gctgagaatg ctgccgaagg
     4741 gatgccgact caacgagtcc ttcggtataa tccgcaggca cacggatcga acagacttca
     4801 catcaaaatt tcaagaaaag agagccaact catgcggcgc cgcacaattt tctttccctt
     4861 cctgcttttt tgcttcgctg cataccttct gccagcgcgg gggcagcttc cgcctgcgaa
     4921 gaaagacccg ctgagcccgg cgcaaagcgc cactggaaaa gcgggatgct ccacaacgga
     4981 ggcatcgtcg tgcgcggagg ccgcggcgaa gattcttccg atcgtgatgg gttcatcgcc
     5041 gatggaggag aatctgcggc ggctgacgga tgaaatcggc gggcgcgtga ccggttcgcc
     5101 gcagatggct aaagccgtgg aatggggcgt gggagcgttt cgcgcggcgg cggtggatgt
     5161 tcacacggag aagtacatgc tgccggtaac gtggagcgaa ggggacacgc ggctcgaatt
     5221 gctcggcccg gtgaaattcc cagtgcggct gaaatcggaa gggtggtcac cggcgacgcc
     5281 agcgggaggg attgaggcga acgtcattga tgtgggatac ggcaacgagg acgattttgc
     5341 gaaggcgggt ggactcgtca agggcgcgat cctattggtc catagcgaca tcggatcaac
     5401 gtgggccgat ctcttcaacg aatatctccg gcctccgatg attatcgaac atgccgtgaa
     5461 ggaaggcgcg agggctattc tgtggatggg cgcgcgggaa agactgctgc tctatcgcca
     5521 cacgaattcg ctggctggtg agatcgacag gattccgcag gcggtcctgg cgcgcgaaga
     5581 tgccatgcgg ttggcgcgga ccgttgaggc ctatccagga aaggtacgcg cgcacttcga
     5641 catgcccaac aaaattggcg gggcgattga gcaggaaaac gttgtcggcg aaattcgtgg
     5701 ctatgagaag ccggatgagg tcgttatcct cggcgcgcat ctggattcgt gggagctggg
     5761 cacgggagcg ctggacaatg gttgcaacgc ggcactggta attgaagcgg cgcgagcgat
     5821 caaggccacc ggacttttgc cgcggcggac gatccgcttc gtgctcttca gcggcgagga
     5881 acaaggcacg gtcgggtcgt tcgagtatgt gaaggtgcat cgcgcggaac tcgacaaact
     5941 tcgcgcgatg atcacgtacg acgcggggat cggccgcgtg acggggtatt cattaggagg
     6001 ccg
//
LOCUS       DASYVO010000367         6564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_206535, whole genome shotgun sequence.
ACCESSION   DASYVO010000367 DASYVO010000000
VERSION     DASYVO010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206535"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VGR03_12345"
     CDS             complement(<1..271)
                     /locus_tag="VGR03_12345"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HEV1995112.1"
                     /translation="MLLTRDFVGYMSRAIVKRLLQQEMIEAGAPDALTAKVRAALADE
                     IGVEDRLNEDVRAILTQYADEMRRTGASYQEMYKKVKNQLARDRKL"
     gene            404..480
                     /locus_tag="VGR03_12350"
     tRNA            404..480
                     /locus_tag="VGR03_12350"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:438..440,aa:Val,seq:tac)
     gene            502..578
                     /locus_tag="VGR03_12355"
     tRNA            502..578
                     /locus_tag="VGR03_12355"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:536..538,aa:Asp,seq:gtc)
     gene            747..2027
                     /locus_tag="VGR03_12360"
     CDS             747..2027
                     /locus_tag="VGR03_12360"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1995113.1"
                     /translation="MSKPQLTRNQIRGFWAAWGGWALDGMDSFIYALVLVPALRELLP
                     RSGIPATTASVGFYGGLLFALFLIGWGLSLVWGPIADRFGRVRTLILTILFFSLFTLL
                     SAFAANVWTLAAFRLLAGIGIGGEWSMGGTFVAEEWPEERRKMGAGMMHTGYYFGFFL
                     AALANYFIGNHYGWRAMFVLGGVPALLVAFIYYGVKEPSRWESKRAQLEQEWGAKRSF
                     FYLFSPEYRRRTILNSTYMLVSIIGLWAGSVYVPASVTFLAAEAGYSLPQGARLASYA
                     TMILSVGTILGCLILPILAERLGRRFTLAIYFLLMLAFIPIGFGYVFYLKEGALFWFM
                     VCLFFLGVGGANFAMYTIWVPEQYRTECRASAFAFSTSVGRFVGAGITFLVGAGVRYF
                     QTIGIPVALTSLAFVVGLLLLPWGEETRGKSLPA"
     gene            complement(2058..2783)
                     /locus_tag="VGR03_12365"
     CDS             complement(2058..2783)
                     /locus_tag="VGR03_12365"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV1995114.1"
                     /translation="MSTFVLVHGSWHDGSAWAPVIRQLEAHGHSAFGPTIAGHGKGAN
                     KRVNHAQCTQSIVDFILSKNLTEVVLVGHSFAGTIIAKVAEAVPDRLRRLVFWNAFVL
                     RDGHCLNDEIPPHYRQLFDDLFKHSADGTVLVPFPIWREAFINDADLALAQSTYALLS
                     PEPYLPFTEKLDMKKFYSLEIPKSYLNCTEDCALPHGEWGWHPRMSGRLGLFRLVQMP
                     GSHETIFTNPVGLAAKIIEAGRD"
     gene            complement(2834..3511)
                     /locus_tag="VGR03_12370"
     CDS             complement(2834..3511)
                     /locus_tag="VGR03_12370"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEV1995115.1"
                     /translation="MPGSPGNSGRSFFTVDPRRAALIVIDMQNAFVAEGAPLETPGAR
                     TMLPRLEGLIRFAREQRIPLVWTQSDHRPPYGGLMLRKFSVIGEQRCLWQGQPSFEMY
                     PHMLQPREGECEYRIVKHKYDAFFETNLDAILRYHHVETLIVTGTATNACCESTARSA
                     FMRDYQVAFPSDANATFDPAMHEATLKNIDLLFGRVMTTAELLTEMEEALDKVSNGAK
                     HAGKARP"
     gene            complement(3547..4614)
                     /locus_tag="VGR03_12375"
     CDS             complement(3547..4614)
                     /locus_tag="VGR03_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEV1995116.1"
                     /translation="MIKPYTAVGLVPTIRGIRSRKDIKINLDHLKHLVKAANWLCALD
                     LPVRLMAIPEGALQAFNDEVLDLDHAQYARECAIDIPGEETEEIGKIAREWDCFIMAQ
                     AKARHPDWKDRFFNVGFILNPKGEVILKHYKVSPLFPVEHSVCPHDIYDWWIEKYGRN
                     LDAFWPVVDTEIGRMGIMMANEGSYPENARALAMNGAEIVYRASYPHPGSGNDFFEIQ
                     SAARALDNNIYVIAPNLGTYFLFPEDTTPIDTFGGRSYIFNYKGQKVGKLEYSGSSSY
                     VGGVIDVEALRDHRARAKWDNWMKDLRTELYQIVYEKPIYPKNLYLDRAPMKHAEYQE
                     KVIQRQIALMHERGIWKKPER"
     gene            complement(4701..>6564)
                     /locus_tag="VGR03_12380"
     CDS             complement(4701..>6564)
                     /locus_tag="VGR03_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV1995117.1"
                     /translation="SSGGFVGSVRNYTEQHFQVAENLTYVHGNHTFKFGGELQPVWID
                     TLVTLFSPGLGIFPPGCFFGVTNQCPVFAPPAFAPGTAVPPGSAVAFLFLEPRQFFGS
                     QVPQRTVTTPLFTGLYSGPAQQAFLDSTRLKFEHTLWSLYAQDHWKIRPNLTLTVGLH
                     YDVDTFPSAKDVKIQGGFHPTNYNNIQPRVSLAYSFNGGKSVVRAGFGIFDSPFVFSD
                     VLVSWIGASEFTYMTNPNLAEFTNPASQLIGFGASGAVGVPSITGLPPFPIGPPLPVQ
                     QLLATGAATAFSNFTHNGIYPSPINPLAPLLQFPLGYAKKKFDNPFAEQASLEIEHQF
                     GKDYFISVGYQFMHATRLPVYYSINGTEAAAPFPACNPAVVSGCQKYFSPVPTDPNFG
                     FTLYVKPSAYSIYHAGTLSVRKSFTHHFNFLANYTYSKSIDISTTINLPNVPMNYLHT
                     NLDRAVGDNNVPHRLTVAALGESPDTWNVVLRNFKASILTNLQAARRYSVVAGFDTNN
                     DHFPFPDRVGTIGRNTYKGDHTYTVDVRLQRVIPFTERIKGEASFEVFNLLNRVNVLE
                     IDHAYGLPDFVTPIPKHYKDGIAPPPSSGGNPGFGSPKFAGEARQIQLSFRLNF"
BASE COUNT         1174 a         1882 c         2138 g         1370 t
ORIGIN      
        1 tgagtttcct atcccgggcg agctggttct tcaccttttt gtacatttcc tggtacgacg
       61 cgccggtgcg gcgcatctcg tcggcatact gggtcaggat ggcgcgcacg tcttcgttca
      121 gccggtcttc gaccccaatc tcatcggcca gcgccgcgcg caccttggcg gtgagggcgt
      181 cgggagcgcc cgcctcgatc atctcctgct ggagcaggcg cttgacgatc gccctggaca
      241 tgtagccgac aaagtcgcgc gtcagaagca tcccggattc ccttcccgtg ccgtgcgcaa
      301 accccgtact gtacttggag gggccgctgc gcgtcaagca agtccatcgg agtggcgagt
      361 cgagcgttga cgctcctaaa agcttcctgt acgatggatt tcggggcggt tagctcagtt
      421 ggttagagca tcccgcttac accgggaagg tcctccgttc aagtcggaga ccgcccacca
      481 gtctggttgt tggcgaccat cggggccgta gttcagttgg ttagaacgcc tgcctgtcac
      541 gcaggaggtc gcgagttcga gtctcgtcgg ccccgccatt tcactgtcgc gccatcgcct
      601 gcgctggaga ctgccctttt tccgtttgcc ccgaccggcc accccgtcga atgcctctca
      661 agaatcgcct gacagagtct atactctccg gcgctcgacc tgccgccgaa gcagcgggct
      721 gacggatcga gggcagaggg atcgcattga gcaaaccgca gttgactcgc aatcagattc
      781 gtgggttctg ggcagcgtgg ggcggctggg cgctggacgg gatggactcc ttcatctacg
      841 ccctggtgct ggtccctgcc ctgcgcgaat tgcttccgcg ctcgggaatc ccggccacca
      901 cggccagcgt gggattctac ggcggtctgc tctttgcgct ctttctgatc ggctggggat
      961 tgtctctggt gtggggaccg atcgcggacc ggtttggccg cgtgcgcacg ctgattctca
     1021 ccattctctt cttttcgctg ttcacgctgc tcagcgcctt cgcggcaaat gtgtggacgc
     1081 tggccgcgtt ccggctgctg gcgggcatcg gaatcggcgg cgagtggtcc atggggggaa
     1141 cgtttgtcgc cgaagagtgg cccgaggagc gccggaaaat gggcgcggga atgatgcaca
     1201 ccggctacta tttcggtttt tttctggccg cgctggccaa ctacttcatc ggcaaccact
     1261 acggctggcg ggccatgttc gtgctgggcg gcgttccggc actgctggtg gccttcattt
     1321 actacggcgt gaaggaaccg agccgctggg agagcaagcg cgctcagctc gagcaggagt
     1381 ggggtgcgaa gcgctccttc ttttatctct tttcgcccga atatcgccgc cgcacaattc
     1441 tcaactccac ctacatgctg gtttcgatca tcggattgtg ggccgggtcg gtctacgttc
     1501 ccgcgtcggt cacgtttctc gccgccgaag cggggtacag tctgccgcaa ggtgcgcggc
     1561 tggcgtcgta cgccaccatg attctgtccg taggaaccat cctgggatgt ttgatcttgc
     1621 cgatcctggc ggagcgactg gggaggcggt tcactctggc catctacttc cttctcatgc
     1681 tggccttcat ccccatcggt tttggctatg tgttctatct gaaggaagga gcgctgtttt
     1741 ggtttatggt ctgcctgttc tttcttggtg tgggcggcgc gaattttgcc atgtacacga
     1801 tttgggtgcc ggagcaatat cgcacggagt gccgcgccag cgcgtttgcg ttttccactt
     1861 cggtgggccg cttcgttggc gcgggaatca catttcttgt cggggccggg gttcgttact
     1921 tccagaccat cggtatcccg gtggcgctga cctcgttggc ctttgttgtg ggtctgctgc
     1981 ttctgccctg gggggaagag acgcggggga aatccctgcc cgcctgaatc caacgaagaa
     2041 acggccggcc ggtagagtca gtcgcgcccc gcttcgatta ttttggcggc gagacccacg
     2101 gggttggtga agatggtttc atggctgccc ggcatctgca ccagacggaa cagtcccagg
     2161 cggccggaca tgcgcggatg ccagccccac tcgccatgcg ggagcgcgca atcctccgtg
     2221 cagttcaggt agctcttcgg aatctccagc gaataaaact tcttcatgtc cagcttctcg
     2281 gtgaacggca gatacggctc cggtgacagc agggcgtagg tcgattgcgc cagcgcgaga
     2341 tccgcatcgt tgatgaacgc ttcgcgccag atgggaaagg gcaccaggac cgtgccgtcc
     2401 gcggagtgct tgaacagatc gtcgaaaagc tgccggtaat gcggcgggat ttcgtcgttc
     2461 aagcaatggc cgtcgcggag gacgaaagcg ttccagaaaa cgagccgccg caagcggtcg
     2521 ggaacggcct cggccacctt ggcgataatc gtcccggcga agctgtgccc cacgaggacg
     2581 acctcggtga gattcttcga caagataaaa tcgacaatcg actgggtgca ttgcgcgtgg
     2641 tttacccgct tattcgcgcc ctttccgtgg ccggcgatgg tggggccgaa cgcggagtgc
     2701 ccgtgggcct ccagttgacg aatgacgggc gcccacgcgc tgccgtcgtg ccatgagccg
     2761 tgcaccagga cgaatgtgga cacgaatcgc tccttgtcag agtcagagat tcaggggatc
     2821 agccaacaga aactcagggg cgcgccttcc cggcgtgctt tgcgccattc gaaaccttgt
     2881 cgagtgcctc ttccatctcc gtcagcagtt ccgcggtggt catcacacgg ccaaacagca
     2941 gatcgatatt tttcagcgtg gcctcgtgca tggcgggatc gaacgtggcg ttggcgtcgc
     3001 tcggaaaggc cacctggtaa tcgcgcatga aggcggaacg ggcggtggat tcgcaacagg
     3061 cgttggtggc cgtgccggtg acgatcagag tttccacatg gtgatagcgc aggatggcgt
     3121 cgaggttggt ttcaaagaat gcgtcgtatt tatgcttgac gatgcggtac tcgcactccc
     3181 cttcccgcgg ctggagcatg tgcggataca tctcgaagct cggctggcct tgccacaggc
     3241 agcgctgctc gccgatcacg gaaaacttgc gcagcatcag gccgccatac ggcgggcgat
     3301 gatccgactg cgtccagacg agaggaatgc gctgttcgcg ggcgaagcgg atgagaccct
     3361 cgaggcgcgg gagcatggtg cgcgcgccgg gcgtctcgag cggcgcaccc tcggccacga
     3421 acgcattctg catgtcgatg acgatcagag ccgcacggcg gggatccacc gtgaagaaac
     3481 tcctgccgga attgcccgga ctgcctggca tggccgacct cctgcccgcc gactggggag
     3541 cgcacgctat cgttcgggct tcttccagat gccgcgctcg tgcatcaggg cgatctggcg
     3601 ctggatgact ttttcctgat actcggcgtg cttcatcggc gcgcggtcga ggtagagatt
     3661 tttcggatag atcggctttt cgtagacgat ctggtaaagc tcggtgcgca ggtctttcat
     3721 ccagttgtcc cacttggcgc gcgcgcgatg gtcgcgcagc gcctcgacgt cgatgacgcc
     3781 gccgacgtag ctcgaactgc cgctgtattc cagtttcccc actttctggc ccttgtaatt
     3841 gaaaatgtag gagcgcccgc cgaaggtatc gatgggcgtg gtgtcttccg ggaaaagaaa
     3901 gtaggtcccc agattgggcg cgatgacgta aatattgtta tcgagggcgc gggcggcgct
     3961 ctgaatttcg aagaaatcgt tcccgctgcc gggatgggga taggaggcgc gataaacaat
     4021 ctcggcgccg ttcatggcca gcgcgcgggc gttctcgggg taggagcctt cattggccat
     4081 catgatgccc atgcggccaa tctcggtgtc caccacgggc cagaaggcgt ccaggttgcg
     4141 cccgtatttt tcgatccacc agtcgtagat atcatgcggg cagacggagt gctccacggg
     4201 aaacagcggc gagactttgt aatgcttcag gatcacttca cctttcggat tcaggatgaa
     4261 gcccacgttg aagaagcggt ccttccaatc gggatggcgc gctttggcct gggccatgat
     4321 gaagcagtcc cactcccggg caatttttcc gatctcctcg gtctcctccc cgggaatgtc
     4381 gatggcgcac tcgcgcgcgt actgcgcgtg atccaggtcg aggacttcat cgttgaaggc
     4441 ctggagggca ccctcgggaa tggccatcaa gcggacggga aggtccaggg cgcaaagcca
     4501 gttggcggcc ttcaccaggt gtttcagatg gtcgaggttg atcttgatgt ccttgcgcga
     4561 gcgaatcccc cggatggtgg gaacaagtcc gacggcggtg tatggcttga tcatggagtt
     4621 ccctgctgga tgcggaaagg ccggcgcccg gaatcagcgc gccggcctcc cgcgtgtctg
     4681 gtgctaatgt cccgggaccg ctaaaagttc agccggaacg acaactgaat ctgccgcgct
     4741 tcgcccgcga acttgggcga gccgaagccc gggttcccgc ccgaactcgg cggcggagcg
     4801 ataccgtcct tgtaatgctt gggaatcggg gtcacgaaat cgggcagccc ataggcgtga
     4861 tcgatttcca gcacgtttac ccggttcagc aggttgaaca cttcgaaact ggcttctccc
     4921 ttgatcctct ccgtgaacgg aatgacgcgc tggaggcgca cgtccacggt gtaagtgtga
     4981 tctcccttgt aggtgttgcg tccgatggtg cccacgcgat cggggaaggg gaaatgatcg
     5041 ttgttggtgt caaatccggc gacgacgctg tagcggcgcg cggcctgcag attggtgagt
     5101 atcgaggctt tgaagttgcg aagcacgacg ttccaggtgt cgggcgactc gccgagagcg
     5161 gccaccgtaa gccggtgcgg gacgttgttg tcgcccacgg cgcggtccag gttggtgtgg
     5221 aggtaattca tcggcacgtt gggcaggttg atcgtcgtcg agatgtcgat cgacttcgag
     5281 tacgtgtagt tcgccaggaa attgaaatgg tgcgtgaagg acttgcgcac gctcagcgtc
     5341 ccggcatggt agatggagta ggccgaaggc ttcacgtaga gcgtgaatcc gaagttggga
     5401 tccgtcggga cggggctgaa gtacttctgg catccgctga cgaccgccgg attgcaggcc
     5461 gggaagggcg ccgccgcctc ggtgccgtta atgctgtagt agaccggcaa acgcgtggcg
     5521 tgcataaact ggtagccgac agagatgaag tagtctttcc cgaactggtg ctcgatttcc
     5581 aggctggcct gttccgcgaa ggggttgtca aacttcttct tggcgtatcc caacgggaac
     5641 tgcagcagcg gggccagcgg gttgatgggg gacggataga tgccgttgtg agtgaagttg
     5701 ctgaatgccg tggccgcccc cgtcgccagc aattgctgca cgggtagggg aggaccgata
     5761 ggaaaaggcg ggaggccggt gatggacggc acgccgacgg cgccggaggc cccgaagccg
     5821 atcaattggc tggcgggatt ggtgaactca gccaaattgg ggttggtcat gtaagtgaac
     5881 tcggaagcgc cgatccagct caccagcacg tcgctgaaca cgaacggcga atcgaagatc
     5941 ccgaaacccg cgcgcaccac gctcttgccg ccgttgaagg aatacgccag gctgacgcgg
     6001 ggctggatat tgttgtagtt ggtgggatga aatccgccct ggattttcac gtctttcgcc
     6061 gacgggaagg tgtccacgtc gtaatgcagg ccaaccgtca gcgtcaagtt gggccggatt
     6121 ttccagtggt cctgcgcgta gagcgaccac aaagtgtgct cgaacttcag ccgcgtcgaa
     6181 tccagaaaag cctgctgcgc gggtccggaa tagagtccgg tgaataacgg cgttgtcact
     6241 gtccgctgcg ggacctgcga gccgaagaac tgccgcggct cgaggaacag gaacgccacc
     6301 gcgctacccg gcgggaccgc tgtgcccggg gcgaaggccg gcggcgcgaa gacgggacac
     6361 tgattcgtga cgccgaagaa gcagcccgga ggaaaaattc cgagcccggg actgaacagc
     6421 gtcaccaggg tgtcgatcca caccggctgc agctcgccgc caaacttgaa ggtgtgattg
     6481 ccgtgaacgt aggtcaggtt ttccgccacc tgaaaatgct gctcggtgta gttgcggacg
     6541 ctgcccacaa agccgcccga cgac
//
LOCUS       DASYVO010000368         6898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_233300, whole genome shotgun sequence.
ACCESSION   DASYVO010000368 DASYVO010000000
VERSION     DASYVO010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233300"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VGR03_12385"
     CDS             <1..719
                     /locus_tag="VGR03_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995118.1"
                     /translation="YSYLQSGQEAKAREVTEHTNHVVGASDEDKAYDRAYLAARTALE
                     LHRWKEAAALPIPAIRKNWQDATIWARAIAAARSGDVAGAESDVKELTQLVADREKRS
                     KKSGYAVSMEKATDLREAEAWLAFAKGKSDEALQELRAAADHQDKGGGESVSIPAREM
                     LADMLMELKRPAEALIEYKTVLKNSPNRFDGLLGAARSAQAAGDSGGAQSYYAQLAQV
                     CAVGADRPELAEAKTYLAQK"
     gene            825..1100
                     /locus_tag="VGR03_12390"
     CDS             825..1100
                     /locus_tag="VGR03_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995119.1"
                     /translation="MQAKTKRIRILALLLGVIFLGAQFHFCTDLTAAPSASHICPVCS
                     TAGSVVATQSPSIAIVPVTNRLEVAPLVVSVSSAVPRATSPRAPPAL"
     gene            1180..2523
                     /locus_tag="VGR03_12395"
     CDS             1180..2523
                     /locus_tag="VGR03_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995120.1"
                     /translation="MKRFAVLSALLLFSSVAHAQDAPSVVVKRDVSPANAEDARMKAL
                     EEQVRTLAGEVALLRGELKELRETKSPEQPSGPQVLLASSHPEPGVLSSSPAPAPAQA
                     TQTQTFGGASGNAKLLNPDISLIGDFVGAAGRNKVSPSPSLEMHESEVGIQAIIDPYA
                     RADAFISFGETGVNVEEAYVTFTSLPAGLLLKVGKKRADFGKVNTIHNHALPFIDRPL
                     VTNNLVGGEDGIDDAGFSLSRFLPAPKNWFLEGTAQVLRGDSADLFQASRREDVSVVG
                     HLRAYRDLTESTNLDLGLSYARGHNNLGSNFLTNLYSGDATLRWKPLRRAIYNSFMFR
                     TELVWSARDQLSAAVPNLFQTQHAFGMYSSAEYRVNRRWTVGGRFDRSGHARDAHLTD
                     SGFSGILTYWPSEFSQIRGQYRFGHLATSPTDFSNANEFLFQFLFVMGAHGAHPF"
     gene            2543..3493
                     /locus_tag="VGR03_12400"
     CDS             2543..3493
                     /locus_tag="VGR03_12400"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEV1995121.1"
                     /translation="MPRLLTRTRFTAIASSFALVLALLCPVVGCADSKIRIMTATTDL
                     ASLAQEIGGDKVDVESVARGYQDPHFVDPKPSFLLKLSKAELLIVVGLELEIGWLPPL
                     ITQSSNPRIQVGAPGYFDASRFAKILELPTGQVTRAEGDVHPLGNPHYWLDPDNGLRI
                     AKGIQNKLSEMRPNDAAYFAQRYDAFEQRLKQSEQQWQAQMKPYAGRKVVTYHRSWPN
                     FADHFGLNVVGYVEPRPGIPPSPQHTVELIGQMKRDGVKIIMVEPYFDLKTPSSIARD
                     TGGQVVVLMPSVGGEKEITDYFKLFDYDIAKLKKAFDETH"
     gene            3505..4401
                     /locus_tag="VGR03_12405"
     CDS             3505..4401
                     /locus_tag="VGR03_12405"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1995122.1"
                     /translation="MEILPFLVLPFLASLILTGIHSYLGVHVVERGVIFVDLALAQIA
                     ALGATVAIIVGMDPHGRGSYWISLGFTFLGAAIFAFARTRRGHIPQEAFIGIAYAVAS
                     AMAILLMSKATGETEHLTHMLVGNILAVSRPEVIKTGLLYSAVGLFHFIFRKKFLLIS
                     MNHNKAEQLGFNVRFWDFLFYASFGFVVTSSVAIAGVLLVFCYLIVPSVGAMLFADRI
                     GPRLAIGWTMGTLVSALGVYLSVKLDTPTGATIVVTFGGVLIVMFLLHLVLHHGRSAR
                     ELKGGVSAQRELETATPSTREA"
     gene            complement(4528..5109)
                     /locus_tag="VGR03_12410"
     CDS             complement(4528..5109)
                     /locus_tag="VGR03_12410"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV1995123.1"
                     /translation="MKMRANLFVAVAMLMAGGVAAAQQAAAPAEAQKMAEMGTIVSPV
                     SGAVKGQLARFSKNMVAAAEAMPAEKYGFKPSPEMNSFGHLVMHIAQSNNLFCSKISG
                     AAPPDVKYAESDPKDKLVAALKASFEYCTTALEKVDDSKLGDQMMLFGNRPASRAAAM
                     IFLYGSWTDHYATEAIYLRLNGILPPTAQPEKK"
     gene            complement(5165..6151)
                     /locus_tag="VGR03_12415"
     CDS             complement(5165..6151)
                     /locus_tag="VGR03_12415"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEV1995124.1"
                     /translation="MRHPTRRRFLAQAGGSAAGAVVWTGVCAGVWPGLFAGMGTALAA
                     EAGPSMRFPAAARERIAIASYPFRDFIYGREDNRGVGGKMELKEFAAHISAKFNIKKI
                     EPWSEHFRSLDKAYLEELRGAVAQAGGAIVNIAVDGEHSPYAADSAERERAITFSKQW
                     IDAAVAIGSPSVRTNIPQAKDAKPDVERTAASLTRVAEHGAAKNVVVNLENDNAVSED
                     PFFLVKVIEKVNSKWLHALPDFANTLAAYEEEYAYRGIDAMFGHAYNISHVKETEAGD
                     GKDKIAHVDLPRTFGIAKQHGYKGYFSMEWDSPGDPYAGTAKLIEKTLQNLS"
     gene            complement(6279..>6898)
                     /locus_tag="VGR03_12420"
     CDS             complement(6279..>6898)
                     /locus_tag="VGR03_12420"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1995125.1"
                     /translation="MKKKTLMLAGLACAVAAVFAVGVRGDAAEEVARLAELMGWKAGM
                     VAADIGAGDGKYTFAAVERVGAAGKVFATEIDAKKLAELRSEVAKRKLANVVVVESKE
                     ADTNLPAGCCDAIFLRRVYHHLTKPVEFDANLARSLKPGGRLAIIDFAPRAGLEPVEG
                     VPSNRGGHGIPQKIVIEELSAAGLQVEKIVNDWPEQSYCVLFVKR"
BASE COUNT         1310 a         2366 c         1711 g         1511 t
ORIGIN      
        1 gctattccta tctgcagagc ggccaggaag ccaaagcgcg cgaagtgacc gagcacacca
       61 accacgtcgt cggcgcaagt gacgaagata aggcctatga ccgcgcttat cttgccgcgc
      121 gaaccgcgct cgaattgcac cgctggaaag aagcagccgc tttgccgatt ccggcgatcc
      181 gcaagaattg gcaggacgcg acaatatggg cgcgcgccat cgcagcggcg cgcagcggcg
      241 acgtggccgg cgcggaatct gacgtcaagg aacttacgca actcgtggcc gaccgcgaga
      301 agcgttccaa aaagagcggc tacgcggtct ccatggaaaa ggccaccgat ctccgcgaag
      361 ccgaagcttg gctggccttc gccaagggaa aatccgatga agctcttcag gaattgcggg
      421 ccgccgccga ccatcaggac aaaggtggcg gcgaatctgt cagcatcccc gcgcgcgaaa
      481 tgcttgcgga catgctcatg gaactcaagc gtcccgccga agccctcatt gaatacaaaa
      541 ccgttcttaa gaattccccg aaccgcttcg acggccttct aggcgccgcg cgctccgccc
      601 aagccgccgg cgactcgggt ggggctcaat cgtattacgc tcaactggca caagtctgtg
      661 ctgtgggcgc cgatcgaccg gagctcgccg aagcgaaaac ctatctcgcc cagaaataaa
      721 gagtcactct catttacgct ccacttgtaa ccgaagcagc actattggag gtgcggaact
      781 tgaccgcgca accattccgg cctaagcttc gtcttagtaa gcacatgcag gctaagacta
      841 agcgcatccg cattttggcg ttgctactcg gggttatctt ccttggggcg cagttccact
      901 tttgtaccga cctgacggcc gcgccctccg cctcgcacat ctgcccggtc tgctcgacgg
      961 caggctccgt ggtcgccaca cagtcgccca gcattgctat tgtgccggtc acgaaccggc
     1021 tcgaagtcgc tccattggtc gtttcggtat cctcagcagt tccgcgcgct acttctcctc
     1081 gcgcgcctcc cgccctctag ctttccgtgc ttctcctata gcggtctcca cgttcgctac
     1141 aacaaaggct caatttcata gaactcttgg aggaatgtcg tgaaacgatt cgctgttctg
     1201 tccgctctgc ttcttttttc ttcagttgcc catgcgcagg atgcgccttc tgtagtggtt
     1261 aagcgcgacg tttcccccgc gaacgcggaa gacgcccgca tgaaggcgct cgaagaacaa
     1321 gttcgcacgc tggctggaga agtagcactc ctgcgcggcg agctaaagga acttcgcgaa
     1381 acgaaatcac cggaacaacc ttccggcccg caagttctgc tagcctcctc gcaccccgag
     1441 ccgggtgtgc tgtcctcctc tcctgctccg gcaccggcac aggccacgca aacacagact
     1501 ttcgggggtg cgtctggcaa cgcaaaactc ctcaatccgg acatcagtct gatcggcgat
     1561 ttcgtcggcg cggctgggcg caacaaagtc tctccgtcgc cttcgctgga gatgcatgaa
     1621 tccgaagtag gtattcaggc catcatcgat ccctatgcgc gcgccgatgc cttcatttcc
     1681 ttcggcgaga cgggcgtaaa cgtcgaagag gcttacgtca ccttcacgtc gctccccgca
     1741 ggcctgttgc tcaaggtcgg caagaagcgt gctgactttg ggaaggtcaa caccattcac
     1801 aatcacgcac ttccgtttat cgaccggccg ctcgtcacaa acaatttagt cggcggcgag
     1861 gacggcatcg acgacgcggg cttctcactc tcgcgttttc ttcctgcgcc caagaactgg
     1921 ttcctcgaag gcaccgcgca ggtattacgt ggtgattctg cggacctgtt ccaggccagc
     1981 cgccgcgagg acgtcagtgt tgtcggccac ttgcgcgcct atcgcgacct gaccgaatcc
     2041 accaacttgg atctcggcct ctcctacgca cgcggccaca acaacctcgg ctcgaacttc
     2101 ctgaccaatc tgtatagcgg agacgcgacg ttacgctgga aaccccttcg ccgcgccatc
     2161 tacaattcct tcatgttccg taccgagctg gtctggagcg cgcgtgacca gctctctgct
     2221 gctgtcccga atcttttcca gacccaacat gcttttggca tgtactcctc cgcggaatac
     2281 cgtgtaaatc gccgctggac cgtcggcgga cgcttcgacc gtagcggcca cgccagggat
     2341 gcccatctga cggattccgg cttttccggt atcctcactt attggcccag cgagtttagc
     2401 cagatccgcg ggcagtaccg cttcggtcat ctggcgacca gtccaactga tttttcgaat
     2461 gccaatgaat ttcttttcca gtttcttttt gtgatgggcg cgcacggcgc tcacccgttt
     2521 taagccgttc caggagaatt cgatgccacg cttattgaca agaactcgtt tcaccgcgat
     2581 cgcctcgtcc ttcgcgctcg tgctcgccct gctctgtccc gtcgttgggt gcgccgacag
     2641 caagattcgc atcatgaccg caaccaccga cttagcatcc ctcgcgcagg aaatcggcgg
     2701 tgacaaggtt gacgtcgaat ctgtcgctcg cggctatcaa gacccccact ttgtcgatcc
     2761 taagccgagc tttcttctca agctctccaa ggccgagcta ctcatcgtcg tgggcctcga
     2821 actcgagatc ggctggctgc caccgctcat cacacagtcc agtaatccga ggattcaggt
     2881 cggcgcgccg ggttatttcg acgcatcgcg atttgcgaaa atcctggaac tccctaccgg
     2941 ccaggtgact cgagcggaag gcgacgttca tcctctgggg aaccctcact attggctgga
     3001 cccggacaac ggcttgcgca tcgccaaggg catccagaac aagctcagcg agatgcgccc
     3061 caacgatgct gcttattttg cgcagcgcta cgatgccttt gagcagcggc tcaagcagtc
     3121 cgagcagcaa tggcaggcac aaatgaagcc ctacgccggc cgcaaggtcg tcacctatca
     3181 ccgttcctgg cccaatttcg ccgaccattt cggcctcaat gtcgtcggct acgtcgagcc
     3241 gcgtccgggt attcccccga gccctcagca caccgtggag ttgatcgggc aaatgaagcg
     3301 cgacggtgtg aagatcatca tggttgagcc gtactttgac ttgaagacgc ccagctccat
     3361 cgcgcgcgat accggcgggc aagtcgtcgt cctgatgcct tcggtcggcg gcgaaaaaga
     3421 gattactgac tacttcaagt tgttcgacta cgacatcgcc aaactcaaga aggcttttga
     3481 cgagactcac taggagagta ttcgatggag attcttccgt ttctggtttt gcctttcttg
     3541 gcgagcttga ttctgaccgg catccattcc taccttggcg tgcatgttgt cgagcgaggc
     3601 gtcattttcg tggacctcgc tctcgcgcag atcgctgcgc tgggcgccac cgttgccatc
     3661 atcgtcggga tggacccgca cggccgcggc tcctactgga tcagcctcgg tttcacgttt
     3721 ctcggcgcag ccattttcgc ctttgcgcgc acccgccgcg gccatattcc ccaggaagcc
     3781 ttcatcggca tcgcttatgc cgttgcctcc gccatggcca tcctcctgat gagcaaggcc
     3841 accggcgaga ccgaacatct cacgcacatg ctcgtcggca atattctcgc cgtctccagg
     3901 cccgaggtga tcaaaaccgg gttgctctac agcgctgtcg gcctcttcca cttcatattc
     3961 cgaaagaagt ttctgctgat ctccatgaac cacaacaaag ccgaacaact cggctttaac
     4021 gtacgtttct gggattttct tttctacgct tctttcggtt ttgtcgttac gtcctcggtc
     4081 gccattgccg gcgtcctgct cgttttctgc tacctcatcg ttccgagcgt cggcgccatg
     4141 ctctttgcgg accgcatcgg tccccgcctc gccatcggtt ggaccatggg aacactggtc
     4201 tctgcgctgg gagtttatct aagcgtgaag cttgataccc ccaccggcgc caccatcgtc
     4261 gtcacgtttg gcggcgtcct catcgtgatg tttctattgc atctcgtcct gcaccacggt
     4321 cgctccgccc gcgaattgaa aggcggtgtt tccgctcagc gcgagctcga aacggccact
     4381 ccctctactc gggaagcgta gtttcccaat cgaaggcagg atcgtgactt ccttgtttct
     4441 gtcttttttc cggaaacgat tatccgtgtt gtgtagggac gcgcagaacc tgtcccgact
     4501 tggtcgggag cgcgtccttc ttttttctta cttcttctcc ggctgcgccg tcggcggcaa
     4561 aatcccgttc aaccgcaaat agatggcttc cgtcgcatag tggtccgtcc agctcccgta
     4621 caagaaaatc attgcagcgg ctcgcgatgc cggccggttc ccgaacagca tcatctggtc
     4681 ccccaacttc gaatcgtcca ccttctcgag cgccgtcgtg cagtattcga acgaagcctt
     4741 cagcgcagcc accagcttgt ccttcggatc gctctcggca tatttcacgt ccggtggcgc
     4801 ggctcccgaa atcttcgagc agaataaatt attcgactgc gcgatgtgca tcaccaggtg
     4861 cccgaacgaa ttcatctccg gcgacggctt aaaaccgtat ttctctgccg gcatcgcctc
     4921 tgccgccgcc accatgtttt tggaaaagcg tgcgagttgc cccttcaccg ccccgctcac
     4981 cggactcacg atcgtcccca tctccgccat cttctgcgcc tcggctggcg ccgccgcttg
     5041 ctgagccgcc gccactcccc ccgccatcaa catcgccacc gccacaaaca aattcgcacg
     5101 cattttcatc gtcattcccc tcgttcggaa tctcagtgga aagaatactc cccaggtata
     5161 tctcttagga aagattctgc agcgtcttct cgatcaactt cgccgtcccc gcataaggat
     5221 cgcccggact gtcccattcc atcgagaagt agcctttgta gccgtgctgt ttcgcgatcc
     5281 caaaagtccg gggcaaatcc acgtgcgcga tcttgtcttt cccatcgccc gcttccgttt
     5341 ccttcacgtg cgagatgttg taggcatgtc caaacatcgc atcgattccc cggtaggcat
     5401 actcctcttc ataagccgcc agtgtgttcg caaaatccgg cagcgcgtgc agccacttgc
     5461 tgttcacttt ttcgatgact ttcaccagga agaacggatc ttcgctgacc gcattgtcgt
     5521 tctccagatt taccaccacg ttcttcgccg cgccgtgctc cgccacgcgc gtcaagctcg
     5581 ccgccgtccg ttccacgtcc ggtttcgcat ctttcgcttg cggaatgttc gtgcgcacgc
     5641 tcggcgaacc gatcgccacc gccgcatcga tccattgctt gctgaacgtg atggcgcgtt
     5701 cccgctccgc gctatccgct gcatacgggc tgtgctcgcc gtccaccgca atgttcacga
     5761 tcgctccgcc cgcctgcgcc accgcccctc gcaactcctc caggtaagct ttgtccagcg
     5821 aacgaaaatg ttcgctccac ggctcgatct tcttgatgtt gaatttggcg ctgatgtgcg
     5881 ccgcaaactc tttcaactcc atcttgccgc cgacgcccct attatcctcg cgcccataga
     5941 tgaaatcccg gaacggatac gacgcaatcg caatccgctc ccgcgccgcc gccggaaacc
     6001 gcatgctcgg tcccgcctcc gcggcaagcg ccgttcccat ccccgcgaac agccccggcc
     6061 acactcccgc gcaaaccccc gtccaaacaa ccgctcccgc cgccgaacca ccagcctgcg
     6121 ccagaaatcg ccttcgtgta gggtgtctca aagccgcgcc agtctaccag actcacctgt
     6181 tgcccagaaa ccaaaaccat gccgtgtcct gcacaagctt gacaaagccg cacagagaca
     6241 gccccgcgcc acacgccttc aacaagcgcg atccgccctc acctctttac gaacaaaacg
     6301 cagtaactct gctccggcca gtcattcact attttttcca cctgcaaccc cgcggcgctc
     6361 agctcctcaa tcacaatttt ctgcgggatg ccgtgtccgc cccggttgct cggaactcct
     6421 tccaccggtt ccaaccccgc gcgcggcgcg aaatcgatga tcgccagccg ccctcccggc
     6481 ttcaacgacc gcgccaggtt ggcatcaaac tccaccggct tggtcaggtg atggtacacg
     6541 cgcctcagaa aaatcgcgtc gcaacacccc gccggcaggt tcgtatccgc ctctttgctc
     6601 tccaccacga caacgttcgc cagcttgcgt ttcgcaacct cgctccgcaa ctccgcaagc
     6661 tttttcgcat cgatctccgt cgcaaatact ttccccgccg cccccacccg ttccaccgcc
     6721 gcaaacgtgt acttcccgtc gcccgcccca atatccgcgg caaccatccc cgccttccat
     6781 cccatcaact ccgccagccg cgcaacctcc tccgccgcgt ccccgcgaac ccccaccgca
     6841 aacaccgccg ccaccgcgca cgccagcccc gcaagcatta aggttttctt tttcatag
//
LOCUS       DASYVO010000369         3219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_6392, whole genome shotgun sequence.
ACCESSION   DASYVO010000369 DASYVO010000000
VERSION     DASYVO010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6392"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2359)
                     /locus_tag="VGR03_12425"
     CDS             complement(<1..2359)
                     /locus_tag="VGR03_12425"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEV1995126.1"
                     /translation="MPLVELIVVLYAILAIATPFITLVLLRKYKKLRENLDQLAEENS
                     RQHASFQREVADLKRQLTAAVHPVTPSVGEPAQRPAVRAAPAVKETPVSTPRVDLPTP
                     VKLPARLSFPTLEKKPEPQLLQKPQEPAPFVPPPLPIAPTGTPAQKLPAPPAPVEPKP
                     VLPAAAKSPAEIKPSTPVAPPQTTPPSVPSSQPRVPRIPVPTPCVPVEIPPAASARVS
                     APPPISAYRVPAPKPTFQQRMKTVSAIEEALGTNWLNKLGIIILVVGVALFGIYELGA
                     LGPLGKVGISYLASGFLLVGGIFLEKNERYRLLGRTGIGGGWALLFFSTFGIYHVEAM
                     RILPLNAGALTLDCALMLIVAVAMALHTLRYRSQFVTGLAFLLGYSTVALSQDTVYSL
                     SAGVFLAVGLVSIVLKMGWFELEVFGILSSYLNHLYWLYRILGIEGAHGRHFEQYHAS
                     LALLFFYWLTFRISYIARKVKTDFEEHVSTLAALLNVLLLLGVMKFQSVQPELAYLAL
                     LFVGAFEFIFAQLPITKRRRQAFVVLSVVGATLMLAAVPFHYSGNNVAILWLVGAEAF
                     LAAGIIAKEVVFRRLGLLTGLLVGLHLVGIDFRGLLNLRLANEDLALAAGVLFSLCAV
                     VFYLNALGVGARWKDLFDDSPDRPLLTVHSYLGAFAIASAAWALFSHDWTALAFAAIM
                     LTLAALGRTLESRDLQVQYALVGALTLYRGIVVNLHLESPQHAHVRMRLLTLPILGAA
                     FYLTARLAALRDDPEQRAIRALFAAAGSAFIGLLIWFEAPELWQPL"
     gene            2781..>3219
                     /locus_tag="VGR03_12430"
     CDS             2781..>3219
                     /locus_tag="VGR03_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1995127.1"
                     /translation="MLNVSEVNAERSSEPLAERASERFSEMLISPYVKERLDAARFTT
                     PTPVQAAAIPQALEGKDVLATAQTGTGKTLAFLIPVIEKLLKDKTPGIAALVLVPTRE
                     LAMQVVDQYNALRGKQLLPAALVVGGLSEGAQLQAIRKGARLVV"
BASE COUNT          762 a          791 c         1074 g          592 t
ORIGIN      
        1 cgaggggctg ccaaagctcc ggggcttcga accagatgag aagtccaatg aaagcgctgc
       61 ctgcggcggc gaagagggcg cgaatggcgc gctgctcggg atcgtcacgc agggccgcca
      121 atctggcggt gagatagaac gctgcgccga gaatgggaag agtgagcagc cgcattcgca
      181 cgtgagcgtg ttgaggggat tcgagatgca gatttacgac gatgccgcgg tagagagtga
      241 gcgcgccgac gagagcgtat tgcacttgca gatcgcgcga ttcgagggtg cggccaagag
      301 ccgcgagggt gagcatgatg gcggcgaaag cgagagcggt ccaatcgtga gagaaaagag
      361 cccaggcagc agatgcgatg gcgaatgcac cgagatagga atgtacggtc agcaggggac
      421 gatcgggcga gtcatcaaat aagtctttcc agcgcgcgcc tactccgagg gcattgaggt
      481 agaagacgac ggcgcagagg gagaagagga cacccgcggc aagagctaga tcctcattcg
      541 ccagacgcaa attgaggagg ccgcggaagt caatgccgac caaatgtagg ccaacgagca
      601 ggcctgtcag gagaccaaga cggcgaaata cgacttcttt cgcgatgatt ccagccgcca
      661 ggaacgcctc cgcacccaca agccagagga tagccacgtt gttgccggaa tagtggaacg
      721 gaacggcggc aagcatcagt gtcgcgccaa ccacgctcag cacgacgaaa gcctgacggc
      781 ggcgtttggt gatgggcaat tgggcgaaga tgaattcgaa tgctccaacg aacaggagcg
      841 cgagataggc gagctccggc tggacggatt gaaacttcat gacgccgagc agcaggagga
      901 cgttcaagag agcggcgagg gtggagacgt gttcctcgaa gtcggtttta actttgcggg
      961 caatgtagga aatgcggaag gtcagccagt agaagaaaag caaagccaag ctggcatggt
     1021 attgctcaaa gtggcggccg tgagctccct cgatgccgag gatgcgatag agccagtaga
     1081 ggtgattcag gtagctggaa agaatgccga agacttcaag ctcaaaccag cccattttca
     1141 agacaatgga aacgagaccg acggcgagga aaacacctgc ggataggctg tagacagtgt
     1201 cctggctgag cgcgaccgtg gagtaaccca gcaagaaggc aagcccagtc acgaattgcg
     1261 agcgatagcg cagagtatgc agcgccatag ccaccgcgac aattagcatg agggcgcaat
     1321 caagcgtgag cgcgccagcg ttcagaggaa gaatgcgcat ggcttcgacg tggtaaatcc
     1381 cgaaggtgct gaaaaagagc agtgcccagc cgccgccgat gccggtgcgg ccgaggaggc
     1441 gatagcgttc attcttttcg agaaatatgc cgcccacaag gagaaaccct gaggcgaggt
     1501 aagagatgcc gactttaccg agcgggccaa gtgcgccgag ttcgtagatg ccgaacagag
     1561 cgacgcccac gacgagaatg atgatgccga gtttgttgag ccagttcgtg cccagggctt
     1621 cttcgatcgc ggacaccgtt ttcatgcgct gctgaaatgt tggcttcggc gcgggtacgc
     1681 gataggcaga gatcggcggt ggcgcggaga cgcgcgcaga tgcagcggga ggaatctcca
     1741 ccggcacgca cggcgtaggc acgggaatgc gcggcacgcg cggctgggac gaaggaacgg
     1801 atggtggagt ggtttgtggt ggagcaaccg gggtagacgg ttttatttcg gctggcgatt
     1861 tcgccgctgc gggcaaaaca ggcttcggtt caacaggtgc gggtggcgct ggcagttttt
     1921 gcgcgggcgt ccctgtggga gcgattggta gaggaggtgg cacgaaagga gcaggctctt
     1981 gcggtttttg cagcagctgc ggctctggct ttttttccaa agtgggaaac gacagccgtg
     2041 caggcagttt cactggcgtc ggtaagtcga cgcggggcgt tgaaactgga gtttccttta
     2101 ctgcaggagc ggcgcggaca gccggtcgct gtgcaggttc accaacagat ggcgtaacgg
     2161 gatggacggc ggcggtaagt tgacgcttaa gatcggcaac ctcgcgctgg aaggaggcgt
     2221 gttgcctgga attctcttcc gcgagctgat ccaaattctc gcgcaatttc ttgtacttcc
     2281 tcagcagaac gagggtgatg aagggagtgg cgatcgccag aattgcatac agcacaacaa
     2341 tcagctcgac aaggggcatt ggggtccttg ggatgcgtga tcctttgttc tgatgttaga
     2401 acgacggcag ataggaatct tacaatttgt ttccagtaca gccttggcag taacctcttg
     2461 cgacttaagg atagtcaaat gaaagactta gcggcgctat ctgatctcac attgggctcc
     2521 agaacaggag gaaaaagacc gttcctactc ggaaatcccc gcacaacgaa ccgagttgtg
     2581 gtatacttcg tctgcgctta atacctcggt tgtgttgtgc ggtccctctg gtgcagtgct
     2641 tgatctgaga ctccccgcct ccagtttcct ccgtaagttt tacgcgtaat ctaaaacaag
     2701 actggcaaag caagctgccg gccgcccaag aatttgtgtg cgcaccgcga cggcttctgc
     2761 cagtaagaaa gtaatactga atgttgaatg tgtctgaagt gaatgcagag cgctcttcgg
     2821 agcctctggc ggaacgcgcc tcagagcgct tttccgaaat gttgatctct ccctatgtga
     2881 aggagagatt ggacgcggcg cggtttacca cgccgacgcc ggtacaggct gcggcaatcc
     2941 cgcaggcgct ggaaggcaaa gatgtgctgg cgaccgcgca gacggggacc ggcaagacgc
     3001 tcgcgttcct gattccggtg atcgaaaaac tgcttaagga taagactcca ggaatcgcag
     3061 cgctggtctt ggtgccgacg cgcgaattgg ccatgcaggt ggtggatcaa tataacgcgt
     3121 tgcgcggcaa gcaattgttg cccgcggcgc tggttgtcgg gggattgtcc gagggtgcgc
     3181 agctccaggc aattcgcaag ggagcgcggc tggtggtgg
//
LOCUS       DASYVO010000370        30134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_64636, whole genome shotgun sequence.
ACCESSION   DASYVO010000370 DASYVO010000000
VERSION     DASYVO010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 30134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30134
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64636"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..2080
                     /locus_tag="VGR03_12435"
     CDS             395..2080
                     /locus_tag="VGR03_12435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV1995128.1"
                     /translation="MTGKTNSELPNSAPLTRGRVEGGKTELLAFTVVVTLGMAARLSL
                     LHSGIWLDEAWVANSILAPTVRQMLYYDRWVQSSPPLFLLLDRVLVHLLGASELSLRA
                     LPWLAAAIALALFAWTLRRIFPFTLALIGTTLLVTNYWAFKYAQQVKQYSTDLLVACV
                     FLCLLSRSLESEAGAGKFLGLLATGSIGVLLSFPAVFWFPAAALVPLRDSILALRQGG
                     GERRARENLAKLILLLVLFSSSLAVTYFFFSVPNQLGSVQEYWRRADLGGGGLFAALF
                     GGFPKNVCRMLFPQRTWSTPISYFLGLLAGAGLVRAMVHGAKREPQALRLLVVTLLPF
                     LAAIAASVAHRYPLRDYPRFILWMLPSCILLILYCLEAVWTRLAGTLRTEWFHLLDRV
                     VAPAGCLVLLCGGLFFLIRYPGRGEDVRSAVMYLKAHAAPADLVFVHGGTSEQFDLYA
                     QLLHWSPGQQYLGKTGWPCCPRHHLQRVSSPRAASFEEDVHSFLDGARGKRVWVIMPA
                     ANPSEWSQGLRYQRIKLPGLLAAHACQEEEEKEFDNVALYTARCNSARNDPGR"
     gene            complement(2188..3474)
                     /locus_tag="VGR03_12440"
     CDS             complement(2188..3474)
                     /locus_tag="VGR03_12440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV1995129.1"
                     /translation="MNASGTSEGFANQKQQALLLVLFFAGSVVLQVLMFQFLPAPRER
                     NRNGDYFGYYEPVALNLLAGKGLVEDDGRAATRYPPGFPLLLTGIFSVADGMRIQRLQ
                     LVGVFNILVSSFDCILVFFIAKFIFTVRVAAISALLWMTYPLQMWLSVQPNSEIPYLP
                     MFLLSVLVALHALGRRQPALGLAAGCLLGFAALFRPAALISTVVMAGALLVLAPRAAK
                     WRGLLASALLLFGFLLFVIPWESYARSKASGEVALVAKGTSNVLDGLTFALPAAQHGE
                     HTPLPEDVWKLMLRIDAQRPQLSSPGKVLSYMFDEFRENPVAVMKLILLKIVRAWFGT
                     YNMHYDYEILIVQLVYLGAFGIGLWRALRQYRDRLPYILFLILVVGSAWASCVLVMPI
                     VRYLVPVMSLVLIIAAVGFDSALFSPRSAPEVVAEA"
     gene            complement(3706..4572)
                     /locus_tag="VGR03_12445"
     CDS             complement(3706..4572)
                     /locus_tag="VGR03_12445"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV1995130.1"
                     /translation="MKKIVVITGANGLIGNRLCQEFVRRGWQVRAGVRRPGEYAATHP
                     DVTPFQCALPDSLDQTAFDGADVCIHAAYATRATTRERARRVNEDGTLAVLSASRRAA
                     VYFVFISSCSAHEAARSDYGRSKLALEKKIDLQRDLVLRPGLVLGKQGLFGRMVDSIR
                     RSRLIPIFDGGRQSVQTIHIADLCAAIVRAVERRLTGRLILAEAESICMRDLLAAIAQ
                     KLGKRAIFISVPATPALVALRVAEGLRLPLPVSSDNLLGLRSMVYQDPTADLARLGIS
                     LRSARQSLDDLL"
     gene            complement(4569..6218)
                     /locus_tag="VGR03_12450"
     CDS             complement(4569..6218)
                     /locus_tag="VGR03_12450"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEV1995131.1"
                     /translation="MADASFDVVVVGSGASAVHAAWPLAEAGLRVAMLDVGNRDEIYA
                     PLIPPKSFSEIRRSDPDQHRYFLGDNFEGISLSRIGLGAQLTPPRMHIIRETEGLAPL
                     VGDNFSAMQSLALGGLAAGWGASTIPFSEGDFASWPIGRRDLQPHYDVVAGRIGLCGA
                     GGDLAPLIGVVDGQLPPADLDSNGESVYLRYQKRRRFFERNGFHVARAWLAVATVPFR
                     GRGPLLYHDMEFWSDGDRSVYRPRYTVEELRGFAGFSYHDRLLVERFEENRASQSVTI
                     TCLNLASGLREQVTARRLVLAAGALGTARIVLRSLNRYDQAVPLLTNPYTYFPSLNVL
                     RLGQPTRDARHGLTQLTMFFEPDGKHERMLQVQMYSYRSLLLFKLVKESPLARRESLR
                     IMRRLYEYFVIFGVFHEDRSSPGNFLKLSSGGGAGHDVLKAHFRLPAEVVHSQRAQEK
                     RIVRILIRLGCLPLKAMRLEYGSSIHYAGCFPMAPDGPPLTTFTTGRLRAAPFVYLAD
                     GSTFPHLPAKSLTFTLMANANRVGTSLCEELEKSAVRPTES"
     gene            complement(6218..6628)
                     /locus_tag="VGR03_12455"
     CDS             complement(6218..6628)
                     /locus_tag="VGR03_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995132.1"
                     /translation="MGTDTERKEFNLFARYLIGQEAPDELLPRYVRANSILFREGCDP
                     KETAAVAFVCRHPRTLPYMDAACGLLRPDHLLRKKLLVIMAILETTPALADHFLSRAT
                     RGIWPLLAEVAWTGLVASVKGIVGLVLFGRYVVR"
     gene            6961..7704
                     /locus_tag="VGR03_12460"
     CDS             6961..7704
                     /locus_tag="VGR03_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1995133.1"
                     /translation="MSPRTDASSQTALTPLLPPVRVSIIIPIYNEILHAKALLDRVLA
                     APLPEGCEKEIIVIDDGSTDGTTQLLKDLSRREIIRFHQSILNFGKGTAVRIGLRIAS
                     GQIILVQDGDLEYDPNDYPRLLEPILSGQASVVYGSRFLGHPEGMQLPNLLANKILTW
                     MANFLYGARITDEATAYKVFRRDVLNRVTLECQRFEFCPEVTAKISRLGVPIYEVPIS
                     YRGRTILEGKKIHWRDGFEAIYTLLKCRF"
     gene            complement(7747..7983)
                     /locus_tag="VGR03_12465"
     CDS             complement(7747..7983)
                     /locus_tag="VGR03_12465"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1995134.1"
                     /translation="MHAADHAELSPTLSHTAMKISVIIPAYNEKAFIEEILRRVQNAP
                     FDKEMVIVDDCSTRFEGGRPSTILASRELGYITE"
     gene            complement(8099..15334)
                     /locus_tag="VGR03_12470"
     CDS             complement(8099..15334)
                     /locus_tag="VGR03_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995135.1"
                     /translation="MEGSAGGSGGGTTTVPPPTLSIAISPTTASIVPNGKLTFSVGVS
                     NATNPAVTLTVNNIPGGNATVGTITSGASVSGTTPTALYQAPAAPPVPNTVMVTATSV
                     QDPTKSATALVVITNTPPAVTVNVTPSAVSVAPGAATPFTTAVTGTANTSVSWAVNGI
                     AGGNNIVGLISPQGVYTAPLAPPSPPSVTVTATSLADPTASGSASVTVQVVVNVTVSP
                     TVANVVTGTPLTFTAVVTGSPNTNVQWSVAGVIGGNATVGTIGITTGVYNAPAAVPAQ
                     ATVTVTATSVADPTKSDSAQLTITAPNQVGVSVAPATTMVAINGIQQFIATVTGTANT
                     LVTWSVNGTPGGTVAAGTITATGLYTAPGAVPIPNTVTVSATSVVANISGTATVQVTG
                     AGAVNVSVAPAVTSIQTSSTQQFAATVTGTTTTTVTWSVNGMNGGNAAIGTISGAGLY
                     TAPASVPTPATVTITATSTASPSSSGSAALTIILLPAPVVVTVAPTSGSLKVTSQFLF
                     TATVSGAPVGQTGVNWSVNGVAGGNASVGTISSAGLYTAPSAVPSPATVQVKATSQFQ
                     SNSSATSNITILPQDTITMTPTAANVQVGLGAQFFVTITGLSNTAVTFKVNGVVGGNS
                     TVGTVSASGLYLAPAVVPTPATVSLTATSVADLTLIATSTITISPLVVVTVAPTTKTL
                     ALSGVQTFTATVTGTANTAVNWSVNGVAPPGNATVGLITNTGQYTAPASLLLTSTFTV
                     TATSVVNPAALANALVTVNVPVGVTVAPSTPTVAVNGTIAFSATVTGATNTAVTWSVS
                     GPANPGTINSASGVYTAPAAPPAGAVTITATSQANPAATGSTTVTVQLPVSVNLTPNP
                     VNVDTGTTQAFTATVMNAVNTAVTWTVNGAPNGNASVGTINPSTGVYTAPNAIPTPAT
                     VTVTATSVADPTKSASSSVTVTPLVTAVSVSPLTPSIATNGTQQFSATVTGTGNLGVT
                     WQVNGTTGGNINTVGSISASGLYTAPSFTVVTTATMFTITAVSVQNPGVSGSTTITVT
                     PPPITVTVSPDPVTVTVNATQQFTVVVTNAQNPAVTWSVVGVAANGTINSVTGLYTAP
                     PAPPAGAVTVLAVSNQNNTKSDTATVTVVSGIAVTVSPAKTNVIVGTTQQFTATVTGT
                     ANQTVTWSVVGGAANGSINVNSGLYTAPATVPAPASVTVVATSAVAPNPNGSTTLTVV
                     PAVVVTVAPTPISLNAGGPAQVFTATVTGTSNTAVTWFVNGVMGGNATVGTIVQATGL
                     YTPPAAVPNPAVVTVTAVSQADPTKSGAASVTIQGVITVSVSPATVGVNINTTQQFSA
                     TVTNASNPAVNWTISGTGNIGSIGLTTGLYTAPSSVPTPATVTVTATSVQNGTSQGTA
                     TITVQPVVSVSISPNAASLNPNQQSQFTATVTGSANTAVTWTVNGIAGGNSTVGLISA
                     SGLYTAPAIVPSSPVVSVTATSAADPTKSASAAVTISAPGAGIFVTVFPRNKRLKMGQ
                     KSQFDSHVFLTPNTAVNWSIGGPSCAGAGNPCGTIDPVTMIFTTPAALPANPNITVTA
                     TSQADPTKSDTQNERLILGVEVMPESADPHITETVQFSAFVRASSNQSLNWFVNGIPN
                     GNATVGTISNSGLYTAPGSVPNPGFVTVSAISVADPTLPGVATIKVYGAKVMKLFPTT
                     VSVLPGWRVNFQHFTNVVFPVTAPSFGLLVNWSVNGVVGGNSTVGTITNYGQYAAPAS
                     PQTVTVAVQSTINPSFTAQATVTITSGAGASAATLLDTFTKVRPYDIISGTASLNVAA
                     AHQEYADWQVLVTANGEDLTGVDVNVSNFTDGLGNVIPSSNATIYFERPLNVFYPSRF
                     QYSDVGEWPDPMVPKVDPFVHETRNAFPFGVNRISPAYKRYPVVNVSDTTNVGMGAGK
                     ATSGGVYTGTVQQRLDMVIDAAGAVGTATFKWSTNGGTTFQQTGVKTSTSPVTLANGV
                     TVSFQAGGVSGVADFNVGDTFWIFAGPLRNQPVWFDLYIPMPTPAGNYTGTVTVTESG
                     KAPQILTVNLQVYTFAIPVSSSFPSRWGAYWQGYEQAHYFVPYDPNLQAQLGKLYGTA
                     CLINRMSCETNTVPNITYSANGMVATADYTGSDQTLAPLANGTITPHGEQLTAINQNL
                     LGGTDTQMYFSTQNQLAHAVAKGWRSRLYDYTYDEPSGPAFPQLTARASLVRSVDITF
                     RTLVTTDISDNLIGFVSRYVPNWVNIGLKEYQRGPTSASRSFYNDPIANGDDLWWYDS
                     CTTQGCGSIGFVPYNDNYPNSMADTSALMNRCWGIMGLIPYQVTGVLYFDSSYSFDLF
                     LGMNPPRVDTWDSIFYFSGNGDGSLFYPGRPSVIGGTTHIPVESFRMKQIRDAFVDME
                     YGLKLQNQGDGAFLATNVLQVVQDIYTYEANPATWAALRKTLGQKIK"
     gene            complement(16002..17027)
                     /locus_tag="VGR03_12475"
     CDS             complement(16002..17027)
                     /locus_tag="VGR03_12475"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEV1995136.1"
                     /translation="MAKAQQLVALPEAGRNGFEAGVLFGQTEEMLRIQRTIERVASIR
                     VPVLIRGESGSGKEVLANHLHRIAPWRDKPCVKVNCAAIPGTLLESELFGYEKGSFTG
                     AYHSRPGKFEMAHGGTMVLDEIADIDSSLQAKLLQVLQDGCFSRIGDLEERRTDVRII
                     SITNREIEGEIQRGSFREDLFYRINVVNLQIPPLRRRLEDLPILVEHFQRYYGERYDR
                     KVQPLPASVIERFRQHQWPGNIRELENYVKRYVILDSAESIVEELEERGRGPVVGGFQ
                     LPPITEFSLKKFSKQASQQAEHYLILEALKQTRWNRKRAADLLGISYRALLYKIKEAG
                     LPPKKRA"
     gene            complement(17343..18596)
                     /locus_tag="VGR03_12480"
     CDS             complement(17343..18596)
                     /locus_tag="VGR03_12480"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEV1995137.1"
                     /translation="MSAEDAFLDLLIETLQSLDRGVRGLFLQKLFKTLAQVDLNETMS
                     LDFWEQILARRRELSESLHKPVSLQTALVDVLASNNLLRMPILMEYEELKKLQINAAT
                     DALTGLYNRRLFEEYFLKELNRSRRHNQHLALVIMDLHCFKDVNDRHGHMEGDHVLQL
                     AASTLRKTLRTSDYAFRIGGDEFALLLPFADPAQASTLARRVRTNYQAALEPLKLSLS
                     LSIDYGVAVFPEDGESREVLIQLADERLYELKNASRSPAPAAQPVLPRASAPPFAPPS
                     PPATPRVIPMEPPAAREPANAAAVSAGPLPAAALPAAAPAPAIFPSLPAARGGERRKW
                     ERVSLVGTRAHAVIGDDGKITARVLDMSYGGVALLVDSAEELPSVFPAVLHVPILPPV
                     KVSLRRAYFQQIDGQKVRIGCAFLP"
     gene            complement(18877..19203)
                     /locus_tag="VGR03_12485"
     CDS             complement(18877..19203)
                     /locus_tag="VGR03_12485"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEV1995138.1"
                     /translation="MMDMSDPERRRSNRIRLQVPMFIRGMDAHGDEFLDLTKTLNISS
                     VGAYLASPRPLKSNEVISLKIPAPSPSNSGLIPEPTPTITARVRRSVAAGDVHLVAVE
                     FLKSLD"
     gene            19574..20794
                     /locus_tag="VGR03_12490"
     CDS             19574..20794
                     /locus_tag="VGR03_12490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV1995139.1"
                     /translation="MDRSPVAERASINSLPFAVVSGALDAVERPVFLVDRTGRICLAN
                     QRALQRLAARGLGDAAEVNLFRDLLGADAHAIFEQLRGGELEVNLPLPPTDDGITRAR
                     IRWLPEPDWFVVILQIARPMTGAANSEMQHTLEELLQEREITYRNLLAAYLRLQEVNR
                     QKTVFLASAAHELKTPLAVMKGYYDLLLTGSLGKLSEKQRDILSESKESCDRLVRLVS
                     MFLNYSALESGRLVLHLRENDLRDCLGELAARWQEAFQRKQVRLETHFSSDLPNFKFD
                     YQKVQQTVANLLDNGLKHTPAGGEVTLNARTHFWERRAASVSPNIERRRTPDEALNSV
                     EISVADSGAGIAPEHHQEIFEDFVRVDRSSSGMGLGLAISKRLIQAHRGKIWVDSELG
                     RGCRFNFLLPTDLE"
     gene            20827..22260
                     /locus_tag="VGR03_12495"
     CDS             20827..22260
                     /locus_tag="VGR03_12495"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV1995140.1"
                     /translation="MSEQEKILVIDDEPSIRKYLETLLQVDGHEVTCVPSGKEALERI
                     GAGERPDLIILDLLMPEMDGLETLRQLMQVERNLNIVMLSCSNEVSNVVEAIRLGAQD
                     YLTKPFEKSELDAAMLKCRQKRELRAENQALRDVVEHLTEDLTFLAASPQMVKIRQQI
                     LQIAPVDVPVFIHGESGVGKEVVARMLHLRSTRAGQQFIKVNCAALPSELLESELFGY
                     EQGAFTGAIRSKPGKFELANKGTIFLDEIAEMSPQLQAKFLHVLQDQQYSRLGGRAMV
                     QVDVRVLAATNVNVREAIRSGRFREDLYYRLNVFSIHVPPLRERTREIPLLFRHFLEK
                     YSEKYQKQPPSPSGHLLEAAMRYPWPGNLRELENFVKRYVILEDDESSFRELLEMAAS
                     RQRSSPGDEPPAREQGLKALVRGLKDEAEMEAIADALEKTNWSRKEAARMLGISYKAL
                     LYKMRQFKLDAGKPVRVTAAAVETPGR"
     gene            22613..22951
                     /locus_tag="VGR03_12500"
     CDS             22613..22951
                     /locus_tag="VGR03_12500"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEV1995141.1"
                     /translation="MTDRRIARRYELSLPVLVRAAAQERKDQRNGRTRDISTRGVYFM
                     IDQDLAPGSALDFMLTLPAEITRGTEVFIHAQGKVVRIENQPRDGERRVGVAAIIEKY
                     DIIRTEPGSP"
     gene            23197..24384
                     /locus_tag="VGR03_12505"
     CDS             23197..24384
                     /locus_tag="VGR03_12505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV1995142.1"
                     /translation="MIISIILLSIAIQTAFFQFLPPALNRNENGDYFSFYGPVASNLE
                     TGNGLLDKRGDFAIYYPPGFPLVLAFQFKVAGILGVEPLSVIAVFNVLISAVSCACVF
                     LIAENVFARRAALLAALAWATYPPNVWLSVQPNSEIPYLPLYFLSVLAALRAIKEMNP
                     KLAVKAGVFVGLAALVRPIALTSAVFLAAGLVMFSPKEFRRRGFASGALLFGAFLLMT
                     APWELCVYAKTGRSLPMFAKGSSVVTQGLTSVITDAPGKPPTPGEVSRSKRGVVGVLI
                     DQLKANPGPILKLLSLKLVRGWYGMYRSRRTNQILPVQLFYLLLAAIGLRRAIRDGRY
                     NLPGIWLLLLSIAGAWLTTVVTVPLLRYMIPQMPLVLIFFAFAFDQLLFEPRRPVRPP
                     IAA"
     gene            complement(24386..25078)
                     /locus_tag="VGR03_12510"
     CDS             complement(24386..25078)
                     /locus_tag="VGR03_12510"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HEV1995143.1"
                     /translation="MPADEPKLRWIARARGGILQHAGPVMRLGVLSGTFNPPTRAHSQ
                     LAETAAWQLALDEVLFVLPEIPPHRSELAAGLADREEMLRRAVAGEPRFSAAVSSHGL
                     LVEIHCALAAHYPPATRAFFLLGRDAAERILVRWPYANREEALVQMFTRFEIAVAERN
                     GRFEIPADDAAARFAGKIHSLTLPSRWDLVSATRVRERVARAESIEDALAPEVAAYVS
                     ARGLYLCAPGKS"
     gene            25189..25623
                     /locus_tag="VGR03_12515"
     CDS             25189..25623
                     /locus_tag="VGR03_12515"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1995144.1"
                     /translation="MNVRDLISTRREVYSLRDDTSVHEAARYLRERQIRSIGIVDATG
                     RLVGALSQSDISDKVAAENKCPAWMKVSEIMTPELVVVPPDTPLDECIRLMEKHGIFH
                     LLVVDEKGTFHGMISVQDLLRVIASDHKARADLLEAMIFPER"
     gene            complement(25662..27149)
                     /locus_tag="VGR03_12520"
     CDS             complement(25662..27149)
                     /locus_tag="VGR03_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995145.1"
                     /translation="MPKTFTITRILFLQGIIFVLASPVTTQRKTHAEPQVHNTVKLPA
                     AAHKKGLTAGFFLDPITGNKVYRLSDRNLCPSGATHFYSYTNQFSPQGRMVFDCLQAR
                     YPPRIFPVYGPDFRLEYDDAIAAAHVPGSGTGLADVQWSQEREVLYARANGMVLELDP
                     IAHKTRGVVNFLQRMEGFVLPNGQKVPITGLDALSVGPGDRLMVHLLCRHSDPGCTPK
                     SGVIGIGVFDPATGKAHALYVPVPGDRAPAGFDEAHWTQNPKGRLVVIYTDAPNFIYN
                     ADLSARVQIDDNHGHTGYFCGSNGRCYLTRVRGDTLPRGGVGQIGCRKPNGDLSPPWQ
                     SEEGLYDDETGKRVLIWGCDVPGQTLYEHIARHAGAKDVLGASTERYTFAPSLEKFFP
                     TDEAIVRGVLEYAGGEPARVRLTPVAYHRSASGPRAQLMGRECDYWAQPRMVMDFTGN
                     RFLFESTMSHPEWPSIEDGKVRTDCVTDVYVAEFSDKPSAPPAAR"
     gene            complement(27184..27351)
                     /locus_tag="VGR03_12525"
     CDS             complement(27184..27351)
                     /locus_tag="VGR03_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995146.1"
                     /translation="MMARWIRRIAFGGSGILMFWQASQSYARAGITTDTAFPAAVGAV
                     MMVLAVTTRGG"
     gene            27551..28990
                     /gene="mtaB"
                     /locus_tag="VGR03_12530"
     CDS             27551..28990
                     /gene="mtaB"
                     /locus_tag="VGR03_12530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEV1995147.1"
                     /translation="MATFHIENFGCRATQADAAAIERQLLDRGCARSADAAHADVVVV
                     NTCTVTAAADAQARDAVRRIHRASPSARILATGCYAQRAPEELAALPGVEWVVGNSHQ
                     TEISRVILPSGSQPGPQPGNAHAESEHFFPLAQLDGVPASLERGPAKILTGDIFALAD
                     LAPENIQGGPILGGEGARTRPILKIQDGCNNRCAYCVIPFVRGRSRSLAPARVVDEIR
                     RLTAAGAKEVVLSGINLGSYGRDLTPRAEFGDLLRRIVDETPVERLRLSSIEPMDVTQ
                     DFIELLASSGRIAPHFHMPLQSASDRILAAMHRWYRAAHYARRMELIAERLPDAAIGA
                     DVIAGFPGETEEDHRRTLDFITALPFTYLHPFAFSKRPGTAGAALADEVPLATIRRRS
                     KELRALGAGKAAAFRAGQAGKTQRVLTLDRSGGDAGGDRWTGALSGNYLNVRVAGRWP
                     ANTWLEVRLTAAGEHLLGAQLAAEEKQKM"
     gene            complement(29143..29520)
                     /gene="acpS"
                     /locus_tag="VGR03_12535"
     CDS             complement(29143..29520)
                     /gene="acpS"
                     /locus_tag="VGR03_12535"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV1995148.1"
                     /translation="MIVGLGIDIAEIDRIEASIRRHGRHFLERIFTSREIAYCERHRN
                     KAERYAGRFAAKEAAMKALGTGWGKGVRWVDIEVVRLPSGKPSLEVAGRAGQIAAGLG
                     VKNISLSITHSGNTAFAEVIFEN"
     gene            29554..>30134
                     /locus_tag="VGR03_12540"
     CDS             29554..>30134
                     /locus_tag="VGR03_12540"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HEV1995149.1"
                     /translation="MKNRKKSARKHSGREKRFAGSAPVRSQPGWGLARSRGIPTLHAK
                     ALARIPWLIHGFSTRQCGASRLGGERVLNLGNTDWDTEEKVGKNRALLMAALGAEKMP
                     LASLQQCHTDILHVVSKPSAEALRGDALLTASLGMLLAVKTADCVPILLADKKRRAIA
                     AVHAGWRGTLARIAEKTVGRMQMEFGTRPRDVVV"
BASE COUNT         5866 a         8949 c         9459 g         5860 t
ORIGIN      
        1 cgcggagtac gccctgctcc tgcggcagct cggcgaagaa gagtttctca acaaagaagt
       61 gggccgtgtg gtggagaagg cctggcgatg gactgcggac ccgcaggcct ggctcgagct
      121 gcgggaaagg ctggagaggc gaattgcgtt gaaagcatcc gcgccgcacc ggtagattca
      181 agcacgcgtc gcggaggagc gcgggtgcga gcaccagcgc agtttttcgc gttgtgtcct
      241 gatcttgaag ggatcgatta tgggcatcgc ccctgcttca gtgggaacca ggcacaagaa
      301 agtttcctgg gagcggcttc ggacgggaga accctgctgg ggggaatcag aattctccgc
      361 atggcgagca tgagcactag gaatcgtgga cgacatgacc gggaaaacaa acagtgagtt
      421 gcctaattcg gcgccgctga cgcgcggacg ggtggaaggc gggaagacag agctcctggc
      481 attcaccgtg gtggtgacgc tgggaatggc cgcgagattg agccttctgc acagcggcat
      541 ctggctggac gaggcctggg tggcgaattc catcctggct cctacagtgc ggcaaatgct
      601 ctactacgac aggtgggtac agagctcccc tccgctgttt ctgcttctcg accgggtcct
      661 ggtccacctc ctcggtgcaa gcgagctctc gctccgggcg ctgccatggc tggcagcggc
      721 tatcgcgctt gccctattcg cctggacgct ccgcaggatt ttcccgttca ccctcgcttt
      781 gattggaaca acgctgctgg ttacgaatta ctgggcgttt aagtatgccc agcaggtcaa
      841 gcagtacagc acggatctcc tggtggcctg tgtgttcctg tgccttcttt cgcgaagcct
      901 ggagagcgag gccggcgcag ggaaattcct ggggcttctg gccacgggga gcatcggtgt
      961 gcttctctct ttcccggcgg ttttctggtt tcccgcggct gcgctggttc ccttgcgtga
     1021 ttcgattctc gctttacggc aaggcggcgg agagaggcgc gcgcgggaaa acctggcgaa
     1081 gctgatcctt ctgctggttc ttttctcgtc gagcctggcg gttacgtatt tctttttcag
     1141 tgttccgaat caactgggca gtgtgcagga gtactggcga agggcagacc taggaggtgg
     1201 cgggctcttc gcggctcttt tcggggggtt tccgaagaat gtgtgccgga tgctgttccc
     1261 gcagcgcacc tggtcaacgc cgatttccta ttttctgggc cttctggccg gcgccgggtt
     1321 ggtgcgcgca atggtgcacg gcgccaagcg cgagccgcag gcgctgcgat tgctcgtggt
     1381 cacactgctt cccttcctgg cggcgattgc ggccagcgtg gcgcatcggt atcctttgcg
     1441 ggattatcca cgctttatcc tgtggatgct gccatcctgc attctgctca tactttattg
     1501 tctagaagcg gtgtggacgc gattggccgg gaccctgcgc acggaatggt tccacctgct
     1561 cgatcgtgtc gtggcaccgg caggatgtct ggtgttgctt tgcggcggtc tgttcttctt
     1621 gatccggtat ccgggccggg gcgaagacgt gcggtccgcg gtgatgtacc tcaaagcgca
     1681 tgcggcaccc gcggatctgg ttttcgtaca cgggggcaca tccgagcagt tcgacctcta
     1741 tgcgcagttg ctgcactggt cccccggcca acagtatctg gggaaaaccg ggtggccgtg
     1801 ctgcccgcgc caccatctcc agcgtgtatc aagcccgcgg gcggcatcgt ttgaagagga
     1861 cgtgcattca tttctcgacg gggcccgggg gaagcgagtg tgggtgatca tgcccgcggc
     1921 caatccaagc gagtggagcc agggcttgcg atatcagagg atcaagcttc ccggattgct
     1981 tgccgcccac gcctgccagg aagaagagga aaaggaattc gataacgtgg cgctttatac
     2041 tgctcgctgc aactccgcgc gaaacgaccc gggacgctaa cttttttcgc gaagtgcctt
     2101 gcgccgaacc ctgcggctta ccgccggcgg aaagccgccc aggggatggc cggaccaatc
     2161 ctcgcgtcac acctcaaaaa cagtgcgtca ggcctcggca acaacttctg gtgcggaacg
     2221 gggggagaag agagctgaat caaaacccac tgcggcaatg atcaaaacca agctcatgac
     2281 cggcaccagg tagcggacga tgggcatgac cagcacgcac gaagcccagg ccgacccaac
     2341 cacgaggata agaaacagaa tgtagggcag ccgatctcga tactgcctta acgcccgcca
     2401 gagaccaatc ccaaacgcgc ccaggtaaac taactggacg atcagaattt cataatcgta
     2461 gtgcatatta taggttccaa accacgcccg gacaattttg agcaggatca acttcatcac
     2521 ggcgacggga ttctctctga attcatcgaa catgtaactc aggacctttc ccggactgga
     2581 gagctgtggc ctctgcgcat cgattcgcag catgagcttc cacacgtctt cgggaagagg
     2641 cgtgtgctca ccatgctgag cagccggcag ggcgaaggta agcccgtcca acacgtttga
     2701 ggttcctttc gcgaccaaag caacttcgcc gctggctttc gaacgtgcgt acgattccca
     2761 gggaatgaca aagaggagaa atccgaacaa caaaagagcg ctggcgagga gccctctcca
     2821 ttttgccgcc cggggagcga ggacgagcag cgcgccggcc atgaccacgg tggaaatcaa
     2881 tgcggcgggg cgaaagagcg ccgcgaagcc caacaaacaa cctgccgcga gtcccagcgc
     2941 cggctgccgc cggcccaacg cgtgcagggc cacgaggact gaaagaagaa acatcggcag
     3001 atacgggatc tcactgttgg gctgaacact caaccacatt tgcagggggt aggtcatcca
     3061 gagcaaagcg ctgattgccg ccacccggac cgtgaagatg aatttggcga tgaaaaatac
     3121 aagaatgcaa tcgaaggagg aaaccaggat attaaataca ccgacgagtt ggaggcgctg
     3181 gatcctcatc ccatcggcga cagagaatat tcctgtcagt agcagcggga atccgggcgg
     3241 atagcgggtc gcagcccttc cgtcgtcctc gacaaggcct ttcccggcaa ggagattgag
     3301 ggccaccggc tcatagtagc caaaatagtc tccgttccgg ttgcgctcgc gcggtgcagg
     3361 aagaaactga aacatcagta cctggagaac aacggaaccg gcgaagaaca acaccagtaa
     3421 aagagcctgc tgcttctggt tagcgaaccc ttcgcttgtc cctgatgcgt tcaccctcac
     3481 ctactcgatc attctgactc tggattcgga gccatctggg agcggaatcg gtctgcaact
     3541 gaagcaagat gcgccaacaa acttgatctt agcacgcgcc caactgttta tgaacagcga
     3601 ggtattcaag accgcatcct cggaagcgat ctgcataccg aaatcctccg ggagtgggtc
     3661 tgccagaatc ccccgttcgc caccatgcag cgaggcattc gggccttaca acaggtcgtc
     3721 aagagattga cgggcggaac gaagagagat gccaagtctt gcgagatccg cggtgggatc
     3781 ctgatacacc atcgagcgca gccccaaaag attgtccgat gaaaccggaa ggggcagcct
     3841 gaggccctcc gcaacgcgaa gagcaacaag cgccggggtg gccggtacgg aaataaagat
     3901 ggctcgtttc cccagcttct gcgcgatggc agcgagaagg tcacgcatgc aaatggattc
     3961 cgcttcggca agaatcaagc gcccggtcaa gcgacgttca acggcccgaa caatcgctgc
     4021 gcacagatct gcaatgtgaa tggtctgcac cgattggcgg ccgccgtcga aaatcgggat
     4081 caaacggctg cggcgaatgg aatccaccat gcggccgaat aaaccctgct tccccaggac
     4141 aagcccgggg cgcagaacca gatcccgctg aaggtctatc tttttttcca gagccagctt
     4201 gctgcggccg tagtcactcc gcgcggcctc atgggcggag caagaagaga tgaaaacaaa
     4261 ataaacggca gcgcggcggc ttgcggaaag tacggccagc gtcccgtctt cgttgacgcg
     4321 ccgcgctctc tcgcgggtgg tggcgcgcgt ggcgtacgcc gcgtggatgc agacgtcggc
     4381 cccgtcgaac gcggtctggt cgagcgaatc cggcagcgcg cattggaatg gcgtcacatc
     4441 cggatgggtt gccgcgtact cgccgggccg ccggacgccc gcgcgaacct gccagccgcg
     4501 gcggacgaac tcctggcaga gacgatttcc aatcagcccg ttcgcgccgg tgatgactac
     4561 gattttcttc acgattcagt cggccgcacc gcagattttt ctaattcttc gcagagcgat
     4621 gtgccgactc gattcgcatt ggccatgagc gtgaaggtaa ggctctttgc ggggagatgc
     4681 ggaaaggtgg agccgtcggc cagatacacg aaaggcgccg cgcgcaaccg gccggtcgtg
     4741 aacgtggtca gcggaggacc gtctggcgcc atcgggaagc acccggcata gtgaatgctc
     4801 gacccatatt cgagccgcat ggccttgagc ggcaggcaac cgagtcgaat caggatcctg
     4861 acgattcgtt tttcctgggc gcgttgggaa tgcaccacct cggcgggcag gcgaaagtgt
     4921 gccttcagga catcgtgtcc ggcacctcca ccgctcgaca atttgaggaa attgccgggt
     4981 gaggaacggt cttcgtggaa gacgccgaag atcacaaaat attcgtacag cctgcgcatg
     5041 atgcggagcg actcacgccg ggccagtgga gattccttga ccagcttgaa cagaaggagc
     5101 gatcggtaag aatacatctg cacctggagc atgcgctcat gcttcccatc gggctcgaag
     5161 aacatagtca actgcgtcag tccgtgacgg gcatcccggg tgggttgacc caggcgcagg
     5221 acgttgagag atggaaaata ggtgtaggga ttggtaagca gcggcacggc ttggtcgtat
     5281 cgattcagcg atcgcaacac gatgcgtgcc gttcccagcg cacccgccgc gagaaccaag
     5341 cggcgcgccg tcacctgctc gcgcaaaccg ctggccagat tcaggcaggt gatggtcacg
     5401 gattgactcg cgcgattttc ctcgaagcgc tccacgagca atcgatcgtg ataggagaaa
     5461 ccagcgaaac cgcgcaactc ttccaccgtg tagcgcgggc gatagacgct gcgatctcca
     5521 tcgctccaga attccatgtc gtgatagagg aggggacctc tcccccgaaa tggcacggtg
     5581 gccacggcga gccaggcgcg cgccacgtga aaaccattgc gctcgaagaa ccggcggcgt
     5641 ttctggtaac gcagatagac ggactcccca ttggaatcga gatcggccgg aggtagctgg
     5701 ccgtctacga ctccgatcaa aggggcaaga tcgcctcctg cgccgcagag accgatgcgc
     5761 ccggcgacga catcataatg gggctgaagg tcgcgcctgc cgattggcca ggaggcaaaa
     5821 tccccttcgc tgaaaggaat ggtagatgcg ccccagcccg ccgcgagacc tcccaaggcc
     5881 aggctctgca tggcgctgaa attgtcgccg acgaggggag cgagaccctc ggtctcccgg
     5941 ataatgtgca tgcgcggtgg tgtgagctgc gccccgaggc cgatccggga gagcgagatg
     6001 ccttcgaagt tgtccccgag aaaatagcgg tgctgatcgg gatcgctgcg gcgaatctcg
     6061 gagaaactct tcggcggaat taatggggcg taaatctcat cgcgatttcc gacgtcgagc
     6121 atcgcaacgc gaagtcccgc ttcggcgagc ggccacgccg cgtggactgc gctggcgccg
     6181 gaacccacga caacaacgtc aaaagaagca tcagccatca gcggacgacg taccttccga
     6241 agaggaccag cccgacgatc cccttcaccg acgcgaccag gcctgtccac gccacttcgg
     6301 ccaagagcgg ccaaattccc cttgtggctc gagacagaaa atggtcagcg agcgcggggg
     6361 tagtttccag aatggccatg atcaccagca gtttttttcg cagaaggtgg tcgggacgca
     6421 gcaaaccgca cgcggcgtcc atgtagggca aggttcgggg gtgtcggcat acaaaagcaa
     6481 cagcggcagt ttcctttggg tcgcagcctt cccggaaaag gatggagttg gctctcacgt
     6541 atcgggggag cagttcgtcg ggcgcttcct gcccgatcag ataccgggca aacaggttga
     6601 actctttccg ttccgtatct gtgcccatcc actcccttgt gttgtcggcg caagcaaaga
     6661 agatttgacc actatcaaag aacttatgca agaatcccgc cgagcgagtt ggaccaagaa
     6721 ttgcctatca aaccgaatga aagctgctgc tcgtcaagtt tcacagctca ctcgacggat
     6781 gagggggaat gtctttgccg caaaatggca atcccgttca caggaattcc caggttgtga
     6841 cggaggatag aactgggcgc ccttgctgga gcggaatgtg cgtggtgggt acgggagtcc
     6901 catctctgca ctcgcggggg gccgggcatt ccgcgttttt ggtaagatga aggtcgttcg
     6961 atgagcccac ggactgacgc aagctcgcag actgcgctga ctccgctctt gcctcccgtg
     7021 cgggtttcca tcatcattcc catctacaat gagattcttc acgccaaggc gctgctggat
     7081 cgcgtgctgg cggcccctct gccggaaggc tgcgaaaagg aaatcatcgt catcgacgat
     7141 ggctccaccg acgggacgac ccagttgttg aaggacctca gccggcggga aattattcgt
     7201 ttccatcagt ccatcctgaa ttttggcaag ggaaccgccg ttcgcatcgg cctgcggatc
     7261 gccagcggcc agattatcct ggtgcaggat ggcgatctcg agtacgaccc caacgattat
     7321 ccacgcttat tggagccgat tctttccggc caggcctccg tcgtctacgg ttcccgcttt
     7381 cttggccacc cggaagggat gcaattaccc aatctactcg ccaacaaaat tctcacgtgg
     7441 atggccaact tcctctacgg cgcgcgaatt acggatgaag ccaccgcgta caaagtgttt
     7501 cgcagggatg tgctcaaccg agtgactctg gaatgccagc ggttcgagtt ttgtccggag
     7561 gtaaccgcca aaatcagccg gctgggagtg cccatctacg aagtccccat cagctaccgg
     7621 gggcgcacca ttctggaagg gaagaagatt cactggcgcg acggtttcga agcgatctac
     7681 acgctgctga aatgccggtt ctgactgcat acgatccggc cggccggaaa caatgcgccg
     7741 ggaggttcat tccgtgatgt aacccaactc tctgctcgca aggattgtgc tgggccggcc
     7801 accctcaaaa cgagtcgaac aatcgtcgac gatgaccatc tctttgtcga atggggcgtt
     7861 ctggacgcgg cgcagaattt cttcgatgaa tgctttctcg ttgtaggccg ggatgatgac
     7921 ggaaatcttc atggccgtgt gcgacagggt tggactgagt tctgcatggt cggcggcgtg
     7981 catgcggcga gtgaactctc tccgtccgcc gtccgttcag accgccacag tctaaaagca
     8041 agctgtcctc ggcgcaaggg gcgcttgccg tttgattgcc cgtttcggaa agcacgggct
     8101 acttgatctt ttgtccgagt gtcttacgga gggcggccca ggtcgcgggg ttggcctcgt
     8161 aggtgtaaat gtcctgcacg acttgaagca cattggtggc caagaaagcg ccgtcgccct
     8221 ggttctgcag cttcaggccg tactccatgt ccacgaaggc atcgcgaatc tgtttcatgc
     8281 ggaaggattc gacggggatg tgcgttgttc ccccgatgac cgaaggccgt ccgggataga
     8341 agagcgatcc gtcaccgttt ccactgaagt agaagatgct gtcccacgta tccacccgcg
     8401 ggggattcat cccgaggaag agatcgaatg agtagctgga atcgaagtag agtacgccgg
     8461 tgacctggta ggggatcagg cccatgattc cccagcagcg gttcatgagg gctgacgtat
     8521 ccgccatgga gttggggtaa ttgtcattgt acggcacgaa tccgatgctg ccgcagcctt
     8581 gcgtcgtgca agagtcgtac caccagagat catccccgtt cgcgattgga tcgttgtaga
     8641 aggagcgcga cgcggacgtg ggaccccgct ggtactcctt caagccgata ttcacccagt
     8701 tcggaacgta gcggctcacg aaaccaatta agttgtcgct gatgtccgtg gtgaccagcg
     8761 tccggaaggt tatgtccacc gaccggacca ggctggcgcg ggccgtcaac tgcggaaacg
     8821 ccggcccgct gggctcgtcg tacgtgtagt cgtagaggcg gctgcgccag cctttggcca
     8881 cggcgtgggc cagctgattc tgggtcgaga agtacatctg cgtatcggtg ccgcccagca
     8941 ggttctgatt gatcgccgta agctgttcac cgtggggagt gatggtgccg ttggcgagcg
     9001 gggccagcgt ctggtcgctc cccgtgtagt cggccgtcgc caccatcccg ttcgcgctat
     9061 aggtgatgtt cgggacggtg ttcgtctcgc acgacatgcg gttgatgaga caggccgtgc
     9121 cgtacaattt tccgagttgc gcctgaagat tggggtcgta cgggacgaaa tagtgggcct
     9181 gctcgtatcc ctgccaatag gcgccccagc ggctggggaa gctcgagctg acgggaatgg
     9241 caaacgtgta gacctggagg ttgacggtga gtatctgcgg ggccttgccg gactccgtca
     9301 ccgttacggt gccggtatag ttgcccgccg gcgtgggcat gggaatgtag agatcgaacc
     9361 agaccggttg gttgcgcagc ggcccggcga agatccagaa cgtgtcgccg acattgaagt
     9421 cggccactcc ggacacacct ccggcctgga aggaaacggt gacgccgttc gccagagtca
     9481 ccggactcgt cgatgttttc acgccggtct gctggaaagt tgtcccgccg ttcgtggacc
     9541 acttgaaggt ggccgtgccc accgcgccag ccgcgtcgat gaccatatca aggcgttgct
     9601 ggacggttcc cgtgtagacg cctcccgagg tcgccttgcc cgcgcccatg ccgacattgg
     9661 tagtgtcgct tacgttgaca accggatacc gcttgtaggc gggcgaaatc ctgttgacgc
     9721 cgaacggaaa agcgttgcgc gtttcatgaa cgaacgggtc caccttcgga accatcggat
     9781 cgggccactc tcccacgtcg ctatactgga accgggaggg ataaaagaca ttgagcggcc
     9841 gctcaaagta aatggtcgcg ttggaagacg ggatgacgtt gccaaggccg tccgtgaagt
     9901 tggagacgtt gacgtccacg ccggtcagat cttcgccatt ggccgtcacc agcacctgcc
     9961 agtcggcgta ttcctgatgt gccgcggcca cattgagtga cgcggtacca ctgatgatgt
    10021 cgtacggccg caccttggta aaagtgtcga gcagggtggc cgcgctggcc cccgctccgg
    10081 acgtgatcgt tacggtggcc tgggcagtga acgaggggtt aatcgtactc tgtacggcca
    10141 cggtgacggt ctgcggcgag gccggcgcgg catattgtcc gtagttggtg atggtgccaa
    10201 ccgtcgagtt tccgccgacg actccgttta ccgaccagtt cacaagcagt ccgaacgacg
    10261 gggcggtcac cgggaaaacc acgttcgtga agtgctgaaa attcacccgc cagcccggga
    10321 gcacggagac cgtggtgggg aataatttca ttacctttgc gccatagacc ttgatcgtgg
    10381 ccacccccgg cagagtggga tccgccacgg agatcgcgga gacggtcaca aagcccggat
    10441 tcggaaccga accgggagcc gtatagagcc cggagttgga gattgtgccc acggtcgcat
    10501 tcccgttcgg gatgccgttc acgaaccaat tgaggctttg attcgaggag gctctgacaa
    10561 acgccgagaa ctgcaccgtc tcggtgatgt gcggatcagc cgactctggc atcacttcta
    10621 cgcccagaat tagccgctcg ttttgcgtat ccgactttgt gggatcggct tgactcgtcg
    10681 cagtgacggt aatgttcgga ttggccggca gagccgctgg ggtcgtgaag atcatggtga
    10741 ccggatcgat tgtcccgcag gggtttccgg ctccggcgca actcgggcca ccgatactcc
    10801 agttcacggc ggtattgggg gtcaggaata catgggaatc gaactgggac ttctgcccca
    10861 ttttaagtct cttatttctc gggaacacgg tgacgaaaat cccggcgcct ggcgcggaaa
    10921 tcgtgactgc tgcgctggcc gatttggtcg ggtcggcggc gctcgtcgcc gtcaccgaca
    10981 ccaccggaga cgatggcacg atcgccggcg ctgtgtacag tcccgacgcg gagatcaagc
    11041 ccacggtgct gttcccgcct gcaatcccat tcactgtcca ggtcaccgcg gtattcgcgc
    11101 tgccggtaac cgtggcggtg aactgcgatt gttgattggg attcaggctc gcggcgtttg
    11161 gtgaaatcga aacgctgacc accggctgga cggtaatggt ggcggtgccc tgcgacgtcc
    11221 cattctgtac gctcgtcgcc gtcacggtca cggtcgcagg cgtcggcaca ctggatgggg
    11281 ctgtatagag tcctgtcgtg aggccgatgc ttccgatgtt tccggtgccg ctgatggtcc
    11341 agttcacggc tggattcgag gcgttggtta cggtagcgga gaactgctgc gtcgtattga
    11401 tattcacgcc cacggtcgcg ggcgacacac ttaccgtgat caccccctga atcgtgacgc
    11461 tggccgcccc cgacttggtt gggtccgcct ggctcaccgc cgtgactgtg accacggcgg
    11521 ggttcggcac ggcggccggc ggcgtgtaca ggccggtggc ctgaacgatc gtgccgacgg
    11581 tcgcgttgcc gcccatgacg ccgttgacga accaagtgac ggccgtattc gacgtcccgg
    11641 tgaccgtcgc cgtaaagacc tgcgcgggtc cgccggcatt caagctgatc ggagtcggag
    11701 caacggtgac taccaccgcg ggcaccactg ttagagtcgt gctgccattc ggattgggag
    11761 cgactgcgct cgtcgccacc acagtgacac tggcgggggc aggaaccgtg gccggtgcgg
    11821 tatagaggcc ggagtttacg ttgatcgagc cgttcgctgc gccgccgacg acactccagg
    11881 tcacggtctg attggctgtg ccggtgacgg tggcggtaaa ttgctgcgtc gtccctacga
    11941 tcacgtttgt cttggccggg ctgacagtga cggcgatccc cgaaacaacg gtcactgtcg
    12001 ccgtatccga tttcgtattg ttctgattac tgacggccag gaccgtgacc gccccggcag
    12061 gcggtgcggg cggggcggta taaaggccgg tcacgctatt gatggttcca ttggcggcca
    12121 cgccaaccac gctccaggtg accgccggat tctgtgcatt ggtaaccacc acggtaaatt
    12181 gttgcgtggc attcactgtc acggtcaccg ggtccggcga cacggtcacc gtaatgggcg
    12241 gcggagttac ggtaattgtt gtcgagcctg agacgcccgg attctggacg ctgacagccg
    12301 taatcgtaaa catggtggca gtggtcacca ccgtgaaact cggtgccgta tacaagccgc
    12361 ttgccgaaat cgagccgacc gtgttgatgt tcccgcccgt ggttccgttc acctgccagg
    12421 tcactcccaa attgcccgtg cccgtcaccg tggcggaaaa ttgctgcgtc ccgtttgtgg
    12481 cgatgcttgg ggtgagcggc gagacggaaa ccgcagtgac cagcggggtg accgtcacac
    12541 tggacgatgc ggacttggtg ggatcggcca cgctggtagc cgtcaccgtc accgttgcgg
    12601 gtgtgggaat cgcgttcggg gcggtgtaca ccccggtcga cgggttgatc gtgcccacgc
    12661 tggcgttgcc gttgggggcg ccgttgactg tccaggtgac tgccgtatta accgcgttca
    12721 tcaccgtcgc ggtgaaggct tgcgtggtgc cggtatccac attcaccgga ttgggcgtga
    12781 gattgacgct caccggcagc tgtacagtga cggtggtgga cccggtcgcg gccggatttg
    12841 cctggctcgt ggcggtaatc gtcacggcac cggccggcgg ggcagcggga gccgtataaa
    12901 cgccgctggc gctgttgatc gtgcccggat tcgccgggcc gctcacgctc caggtgaccg
    12961 ccgtattcgt ggcccccgtg accgtggcgc tgaaagcaat cgtgccgttc actgctacgg
    13021 tcggcgtgga aggcgcgacg gtgactccga ccgggacgtt cacggtcacc agcgcgttgg
    13081 ccagcgcggc ggggttgacc acgctggtcg ctgtcacggt aaacgtgctg gtcagcagca
    13141 ggctcgcggg ggctgtgtac tgcccggtgt tcgtaatcaa gcctaccgtc gcattcccgg
    13201 gcggggcgac tccgttcacg ctccagttga cggcggtgtt ggcggtgccg gtaaccgtgg
    13261 cggtgaacgt ctgaacaccg ctcagcgcca gcgtcttggt cgtcggtgcc accgtgacga
    13321 ccaccagagg actgatggtg atcgtcgacg tcgcaatcaa agtgagatct gccacgctcg
    13381 tggcggtaag cgacaccgtg gccggtgtcg gcaccacggc gggagccaga tagagcccgc
    13441 tggcgctgac cgttcccacc gtggagttgc cgcccaccac cccattcacc ttgaatgtca
    13501 ccgccgtgtt gctcaatccc gtgatggtga cgaagaactg cgcccccagg cccacctgca
    13561 cgttggctgc ggtcggcgtc atggtgatgg tgtcctgcgg aaggatggtg atgttcgagg
    13621 tcgccgacga gttgctctgg aactggctag tggccttgac ctggacggtc gcgggggagg
    13681 gcaccgcaga gggcgcggta tagagacccg ccgagctgat cgtgcccacc gaggcgttgc
    13741 cgcccgctac gccattcacg ctccagttga ccccggtctg tcccaccggc gccccgctca
    13801 ccgtagcggt gaacaggaac tggctggtca ccttcaagct cccgctggtc ggcgccaccg
    13861 tgaccaccac gggcgcgggc agcaggatga tggtcaacgc cgcgcttccc gacgagctgg
    13921 gcgacgccgt gctggtagcg gtgatggtga ccgtggcggg cgtcggcacg ctcgccggcg
    13981 cagtatagag gcccgcccca gagatggttc caatggccgc gttgccccca ttcattccgt
    14041 tgacgctcca cgtgaccgtc gtggtggttg tgcccgtgac cgtcgccgcg aactgctgcg
    14101 tggaactcgt ctggatggag gtcaccgcgg gcgcgacgct gacgttaacg gcgccggccc
    14161 ccgtcacttg cacggtggcg gtgccggaga tgttggcgac gacgctggtc gccgaaacgg
    14221 tgaccgtgtt gggaataggc accgcccccg gcgcggtgta cagcccagtc gccgtaatgg
    14281 tacccgccgc cacggtgcca cccggggttc cgttgacgct ccaggtgacc agcgtattcg
    14341 cggtgccggt gactgtggcg ataaactgtt ggatcccgtt aatggccacc atcgtcgtgg
    14401 ctggagccac cgacacgccg acttggttcg gcgccgtgat ggtcaactgc gcgctgtcgg
    14461 acttggtggg gtcggccacg ctggtggccg tgaccgtcac cgtggcttga gcgggcaccg
    14521 ctgccggcgc gttgtatacg ccggtggtga ttccgatggt gcccaccgtg gcgttgccgc
    14581 caatcacgcc ggccacactc cactgcacat tggtgttcgg cgagcctgtg accacagcag
    14641 tgaaggtcag cggggtcccg gtcaccacat tggctaccgt gggggacacg gtcacgttta
    14701 ccaccacttg cacggtcacc gatgccgatc ccgacgccgt tgggtccgcc aggctcgtcg
    14761 ccgtcacggt aacgctcgga ggggaaggcg gcgccaaggg agccgtgtag acgccctgcg
    14821 gagaaatcag accaacgata ttgttgcccc ccgcgatgcc gttcacggcc caactcacgg
    14881 acgtgttcgc cgtgccggtc accgcggtcg tgaagggcgt cgccgcgccc ggagccacgg
    14941 acaccgcgga cggagtcacg ttgaccgtga cggcgggcgg tgtgttggtg atcaccacca
    15001 gcgcggtcgc ggacttcgtc ggatcctgca cgctcgtcgc ggtcaccatc acggtgttgg
    15061 gaaccggagg cgctgccgga gcttgatata aggcggtcgg tgttgtgccg cttaccgagg
    15121 caccgctggt gatggtgccc actgtggcgt tgccacccgg aatgttgttc acggtgagag
    15181 tcaccgcagg gtttgtcgcg ttggagaccc ccacggagaa cgtcaatttg ccgttgggta
    15241 caatcgaggc tgtggtcgga ctgatggcga tggagagggt gggaggaggc accgtggtcg
    15301 tccccccgcc tgagcctcct gcgctgcctt ccacttgtcc gccgctggag cagttggaaa
    15361 gtgtcaggat gcttccaaac gcgagcaaac acaaaaagag gcggattgat ggccacgaga
    15421 aacgggaatg ctcacccaac cgtcgggcgg caccgtgtgt ggagcggggg ccagagggcc
    15481 gaaaaaccga caactgcccc cccgcattat cagcgaagat cctgtccatc acgagagtga
    15541 tacttccccg ggccgggtcg ctcgagcggt atcagaatag aatcattgtg ctgcgcgatg
    15601 caatgcacaa agaccggcct actttatctt tagagcacgt agggtactag ggcgtccggc
    15661 gggtagcaaa tcatttttac atgaaagagc ttggcagggg gtgcagcgta gtggaagggc
    15721 agggtttagc agggacccgg ggcggagagt ctttcgccag ggttcacttc cttaagtgct
    15781 gaagtggagg tcgtgaatcc gccgaaacag cagaaaaact tcggaatcca agcctcgagc
    15841 aggcggcgca ctctaggatt gcgttgcggc ctcaggagcc caacccagga gagagccctg
    15901 ccggagcggc tgggcgcgaa agcgggttgc ctacgacgga cctgccgcgg gggaggaatc
    15961 tttgagcatt gctgcgaggg gcggcgcgct ccgttcgcgt cttaggcgcg tttcttgggg
    16021 ggtagtccgg cttccttgat cttgtagagc agcgcgcgat aactgattcc cagcagatca
    16081 gccgctcgct tgcgattcca gcgcgtctgc ttcagtgctt cgagaatgag gtagtgttcc
    16141 gcctgctggc tggcctgttt cgagaatttc ttcaaagaga attccgtgat cggcggaagc
    16201 tggaatccgc ccacaacggg accgcgtccg cgctcctcca gctcttccac gatcgattcc
    16261 gcagagtcca ggatcacata ccgcttgacg tagttttcga gctcgcgaat gttgccgggc
    16321 cattggtgct gccggaagcg ctcgatgacc gacgccggga gaggctgtac tttgcggtcg
    16381 tatcgctcgc cgtagtaacg ctggaagtgt tccaccaaga tcgggaggtc ttccaggcgc
    16441 cggcgcagtg gaggaatctg caagttcact acgttgatgc ggtagaaaag atcttcgcga
    16501 aatgatccgc gctggatctc tccttcgatt tcccggttgg tgatggagat gatgcgcacg
    16561 tcggtgcggc gctcttcgag gtcgccgatg cggctgaagc agccgtcctg cagcacttgc
    16621 aacagcttgg cttgcaaact tgagtcgatg tccgcgatct cgtcgagcac catcgtgccg
    16681 ccgtgggcca tctcgaactt gccgggccgg ctgtggtaag cgcccgtaaa cgatcccttc
    16741 tcgtaaccga acagttcgga ctcgagaaga gttccgggaa ttgccgcgca gtttaccttc
    16801 acacacggtt tatcgcgcca gggcgcgatg cggtgcagat ggttcgccag aacctctttg
    16861 cccgatccgc tctcgccgcg gatgagcacc ggcacccgaa tcgaggcgac tcgctcgatc
    16921 gtccgctgga tgcgcagcat ctcctccgtc tggccgaaga gaaccccggc ttcgaagccg
    16981 ttgcgccctg cttcggggag tgcaacgagt tgttgtgctt tagccacgga ttttcaaaat
    17041 ctcgaccgat acctgcaaca attcaatgca cttagttcgc caagttccag gcggctttcg
    17101 gcaagccaaa aatcaccgct aaggataact cctataagca gttacatata gcttggtcga
    17161 tacaactgcg agtcaagtgt gcacctcgac tgtatgaagc caagttctct catgtgcaaa
    17221 actttgccta ttttaccatg cgctgccagt ccttcgcgcg ggggaggcac gaaagggtaa
    17281 ccgggcaaag tccttcgaag ggtagccagg cgcagtctgt cggatgaggg cagatgatga
    17341 ggttaaggga ggaaggcgca accgatgcga accttctgtc cgtcgatctg ctggaagtaa
    17401 gcgcgacgca gactgacctt cacgggcggc agaattggca cgtgcagcac ggccggaaag
    17461 acgctgggca gctcttcggc gctatccacg agcagtgcca cgccaccgta gctcatgtcc
    17521 aggacgcgtg cagttatctt cccatcatcg ccgatgacgg cgtgggcgcg cgtgcccacc
    17581 agcgatacgc gctcccactt gcggcgctcg ccccctcgag ctgccggaag gctcgggaag
    17641 atcgccggag cgggggcagc tgctggcaaa gcggctgccg gcaaaggtcc ggcagagact
    17701 gccgcagcat tcgcgggctc ccgcgcggca ggcggttcca tggggatgac gcgcggcgtc
    17761 gccggcgggg atggtggtgc gaacggggga gccgacgcgc gggggagaac gggctgcgcg
    17821 gccggtgcgg gagagcggct cgcgtttttc aattcgtaga gccgctcgtc ggccaactga
    17881 atgagcactt ctctcgattc gccgtcctcg ggaaaaaccg cgacaccgta gtcgatggac
    17941 agagaaaggg aaagcttgag gggctcgagc gcggcctgat aattggtgcg cacgcggcgc
    18001 gccagcgtgg aggcctgggc cggatcggca aacggcagca gcagcgcgaa ttcatcaccg
    18061 ccaatccgga atgcgtaatc ggaggtgcgc agcgtcttgc gcagcgtgct ggcggcaagc
    18121 tgcagcacat ggtcaccctc catatgcccg tgccggtcgt tcacatcctt gaagcagtgc
    18181 agatccatga tcaccagcgc cagatgctgg ttgtgccgcc gcgaacgatt cagctccttc
    18241 aagaaatact cttcgaacag ccgccggttg tagagtccgg tgagcgcatc cgtggccgcg
    18301 ttgatctgga gcttcttgag ctcctcatac tccatcagga tgggcatgcg cagcaaattg
    18361 ttggaggcca agacatcgac cagcgccgtc tgcagcgaga ccggcttgtg gaggctttcc
    18421 gagagttcgc gccggcgcgc cagaatctgc tcccagaagt ccagactcat cgtctcgttc
    18481 agatccacct gggcgagggt cttgaaaagc ttctgcagga ataaaccgcg caccccgcgg
    18541 tctaagctct gaagggtctc aatcaacaga tcgaggaagg cgtcttctgc ggacatttgc
    18601 agacgctgca tctccgagtt ggtcgttgcg ggcgcagcct gactcacgct cgctacactc
    18661 cggcgcaaaa agcgacaagc gcgctacacc tcaaggagca agcggcatac cactcgggaa
    18721 gcgtggctgg tcgcttgggg ggcatcctaa gtgcctgtag gttcaatgtc aatgaaacgt
    18781 caattggaaa tgccgcggcg tagtggaagg acatttcggc ggaagaggaa aacagttgca
    18841 tcaggccgag gacctggcct ctcccagcag gtcggcctaa tcgagagact tgaggaattc
    18901 gacggcgacc agatgaacgt ctcccgcagc caccgaacgg cgcacgcggg ctgtaatcgt
    18961 tggtgtcggt tccgggatca agcccgagtt tgagggcgac ggcgcgggga tcttcaaaga
    19021 aatcacttca ttcgacttca gcggccgggg actggctaga tacgccccta cagagctgat
    19081 attcagggtt ttagtcaagt cgaggaattc atccccgtgg gcgtccatcc cacggatgaa
    19141 catgggcact tgcaaccgta ttctgttcga acggcggcgc tcgggatcgc tcatgtccat
    19201 catggcccca ctactatatc cggaactcta aaacggccgg gatagtaccc taggtctagt
    19261 actgaaatac accgcaattc tcgtgcaagt cggtaagaac cggagagatt taacacgcct
    19321 attattatca atgagttgca gggatcgccg ggagcggtat tacccgagtt ttagaaaact
    19381 ctaggcctcc aactcccact cttttcccct aatgtgcaag agacaggact actttcccat
    19441 tgaccgtgtg tgagtgttct gcaagcatcg cttggttgag gtcaatcctt ccgggccgcg
    19501 gcttgcgggt tctttcttcc cctctgccgc cttagaaggg agacgcaccc ggggttccaa
    19561 gaaactcacg aatatggatc gaagcccagt cgcggaacgc gcgtcgatta actcactgcc
    19621 gttcgcggtt gtcagcggcg ctctggatgc tgtcgagcgc ccggtgttcc tggttgaccg
    19681 caccggacgc atctgcctgg cgaaccagcg cgccttgcag cgcctggccg cccgtggttt
    19741 gggcgacgcg gcggaggtca atctgttccg cgacctcctg ggcgcggatg cgcacgcaat
    19801 tttcgagcag ctccgtggcg gcgagcttga agtgaatctc ccgctccccc ccaccgacga
    19861 cggcatcacg cgcgcccgca tccgctggct gccggagccc gactggttcg tggtaatcct
    19921 gcagattgcg cggccgatga cgggcgccgc aaattccgag atgcagcaca cgctcgaaga
    19981 gctgctccag gaacgcgaaa tcacttaccg aaatctgctg gccgcctatc tgcggttgca
    20041 ggaagtgaac cggcagaaaa ccgtcttctt ggcttctgcg gcgcacgagc tgaaaactcc
    20101 tctcgccgtg atgaagggct attacgacct gctgcttacc gggtcgctgg gaaaactgag
    20161 cgagaagcag cgcgacatcc tgtcggaatc gaaggagagc tgcgatcgcc tggtgcggct
    20221 agtgtccatg ttcctgaact actccgccct ggaaagcggc cggctggtgc tacatctgcg
    20281 cgaaaacgat cttcgcgact gcctgggtga gctggcggcc cgctggcagg aggcatttca
    20341 gcgcaaacag gtgcgactgg agacccactt ttcgagcgac ctgcccaact tcaagttcga
    20401 ctatcagaag gtgcagcaga cagtggccaa tctgctcgac aacggcctga aacacacgcc
    20461 ggcgggcgga gaggtgacgc tcaacgcgcg gacgcatttc tgggagcgcc gcgccgccag
    20521 cgtgtcgccc aatatcgagc gccgccgcac gcccgacgaa gcgctcaaca gcgtggagat
    20581 ttcggtggcc gattcgggag cgggcatcgc gccggaacat caccaggaga tcttcgagga
    20641 tttcgtgcgg gtggaccgct cctcgtcggg catgggcctg ggactggcca tcagcaagcg
    20701 cctgattcag gcgcaccgcg ggaagatctg ggtggacagc gaattgggcc gcggatgcag
    20761 attcaatttc ctgttgccta cggatctgga gtagcccggc gcccggtggg aaatactgag
    20821 gagactatga gcgagcaaga gaaaattctg gtaatcgatg acgagccgag catccgcaag
    20881 tatctggaga ccctgctcca ggtggacggc cacgaagtga cctgcgtccc ctccggcaaa
    20941 gaagcgctcg agcgcatcgg cgcaggagaa cgtccggatc tgatcattct cgatctgctg
    21001 atgccggaga tggacggcct cgagactctt cgccagctca tgcaggtgga gcgcaacctc
    21061 aatatcgtga tgctctcctg ctcaaacgaa gtgagcaatg tggtggaggc catccgcctg
    21121 ggcgcgcagg actacctgac caagcccttc gaaaaatctg aactggacgc ggcaatgctg
    21181 aagtgccggc agaagcgtga gctccgcgct gagaaccagg cgctgcgcga cgtcgtcgag
    21241 cacctcaccg aggacctcac ctttctcgcc gccagcccgc agatggtgaa aatccgccag
    21301 cagatcctgc agatcgctcc ggtggacgtt cccgtcttca tccacggcga gagcggtgtg
    21361 ggcaaggaag tcgtggcgcg catgttgcat ctgcgctcga cgcgcgccgg ccagcaattc
    21421 atcaaggtca actgcgcggc gctgcccagc gagctcctcg aatccgagct gttcggctac
    21481 gagcagggcg ccttcaccgg cgccatccgt tccaagcccg gaaaattcga gctggccaac
    21541 aagggcacca tcttcctcga tgagatcgcg gagatgagtc cgcagcttca ggccaagttt
    21601 ctgcacgttc tgcaggacca gcagtattcg cggctcggtg gccgcgccat ggtccaggtg
    21661 gatgtgcgcg ttctcgccgc caccaacgta aacgtgcgcg aggccatccg gtcgggacgc
    21721 ttccgcgagg acctttacta ccgcctgaat gttttttcca tccacgtgcc gccgctgcgc
    21781 gagcgcacgc gggagattcc gctgctgttc cggcatttcc tcgaaaagta cagcgagaaa
    21841 tatcagaagc agccgcccag cccttccggg cacctgctcg aggcggccat gcgttatccc
    21901 tggccgggca acctgcgcga gctggagaat ttcgtgaaac gatacgtcat cctggaagac
    21961 gatgaatcga gcttccgcga gttgctggaa atggcggcca gccgccagcg ctcttctccg
    22021 ggcgatgagc ctcccgctcg cgagcagggc ctcaaggccc tggtgcgcgg cctgaaggac
    22081 gaggcggaaa tggaagcaat tgccgacgcc ctcgaaaaaa caaattggag ccgcaaagaa
    22141 gcagcgcgca tgctgggcat cagctacaag gcgttgctct ataaaatgcg ccagttcaag
    22201 ctggacgccg gaaagccggt gcgggtcacg gccgcggcgg tggagacgcc aggccgctag
    22261 ccggcgggtg caagcgtttg cacattttcg gcagattgcg gcgaagaaaa ctaggtgtta
    22321 ccgtgcctcc acgttatcat ccataatgga acgcaggctg ccgcgcccgg gacacccagc
    22381 cacgcccgcg aaggccaggg gccaaggttt atgaatcgaa cgctatcatt tttcttttca
    22441 agcgtctcga cgggcttcga ggggaagcgc ggtaaacggt aacaggggaa gacaggcaga
    22501 aacaggggac gcaagctctt ctccatcttc caggttttgg aggagggatt gctcctcagt
    22561 accttgtccg gggctccagg tcgaaatatg cgccactggg gggagcttgg gaatgactga
    22621 tcgcagaatc gcaagacgtt acgaattgtc actgccggta ctggtacgcg cagccgcgca
    22681 ggagcggaag gatcaacgca atggccgcac ccgcgacatc tccacgcgag gcgtctattt
    22741 catgatcgat caggatcttg cgccaggaag cgcacttgat tttatgctca cgcttccggc
    22801 agaaatcact cgcggcacgg aagtgttcat ccacgcgcag ggcaaagtgg ttcgcataga
    22861 aaatcaaccg cgggacggcg agcggcgcgt gggcgtcgcg gccatcattg agaaatacga
    22921 catcattcgg accgagcccg gctctccata aaacgctcgc ggtcctgaaa agacgactga
    22981 cttgaccagt tcgtccatcc aacgtgaatc ccttctttct ctgcgcccgg gtccggcagc
    23041 ggcattctac cttttccttt ttgtcggagc cgggattcac tccgcattca acgccggggg
    23101 aggactttag tggaaccggc gccaaggcag ggtcgttctg cgccgctgca acggctgggg
    23161 aaaatcctcg atgaattttg cagtcatcga agatgggtga ttatcagcat cattctcctg
    23221 tcaatcgcaa tccagactgc cttcttccaa ttccttcccc cggccctgaa ccggaacgaa
    23281 aacggagact acttttcctt ttacggccct gtggcgtcga atctggaaac cgggaatggt
    23341 cttctggaca agagggggga cttcgccatt tattacccgc ccggcttccc gctggtgctg
    23401 gcttttcaat tcaaggtggc gggcatcctg ggagtcgaac cgctttccgt cattgctgtt
    23461 tttaatgttt tgatttcggc agtgagctgc gcgtgcgtct tcctcatcgc cgaaaacgtc
    23521 ttcgcgcgga gagcggctct tcttgctgcc ctggcttggg cgacttaccc gccgaatgtc
    23581 tggctaagcg ttcaaccgaa cagcgagata ccgtatcttc ctctttattt tctgtcggtg
    23641 cttgcggcgt tgcgtgcgat aaaggaaatg aatccgaagc tggcggtgaa agcgggagtg
    23701 ttcgtcgggc tggcggccct ggtgcgcccg attgccctga cctcggccgt gttcctcgcg
    23761 gcgggcctgg tgatgttctc tcccaaggaa tttcgccgga gaggattcgc ttccggagca
    23821 ctcctctttg gggcgtttct actcatgact gcgccctggg aattatgcgt ctacgcgaag
    23881 accggtcgat cgcttccgat gttcgcgaag ggttcgtcgg tcgttacgca ggggctcacc
    23941 tccgtcatta ccgatgctcc gggaaagccc cccacccccg gggaagtcag tcgatccaag
    24001 aggggcgtcg tcggtgtgct catcgaccag ttaaaggcca acccggggcc gattctgaag
    24061 ctgctgtctc tgaagcttgt caggggctgg tatggcatgt accggagccg aagaacgaat
    24121 caaatccttc cggtgcagct tttttatctc ctgctggcag cgatcggact gcggcgcgcg
    24181 attcgggatg gcaggtacaa cttgcccggc atctggctgc tcctcctgag catcgcggga
    24241 gcatggctca cgacggtcgt taccgtaccg ctgctgcgat acatgattcc ccagatgcct
    24301 ttggtcctga tcttttttgc ctttgcgttt gatcaacttc tcttcgagcc gcggcggccc
    24361 gtccgtcctc ccatcgccgc ctgacttagc tcttgccggg ggcacaaaga tagagcccgc
    24421 gcgccgagac atacgccgcc acttccggcg ccaacgcatc ctcgatagat tccgcgcgcg
    24481 ccacgcgttc tcgaactcgc gtggcagaga ccaggtccca tcgagacggc agagtgagcg
    24541 aatgaatttt tcctgcgaag cgcgctgccg catcgtccgc gggaatctcg aatcgcccgt
    24601 tgcgttcggc caccgcgatc tcgaaacggg tgaacatctg caccagcgct tcttcccggt
    24661 tcgcgtacgg ccagcggacc aggattcgct cggcggcatc tcttcccaga agaaagaagg
    24721 cgcgggtggc cggtggatag tgcgccgcca gagcgcagtg aatctcgacg agcagtccat
    24781 ggctggagac cgcggcggaa aatcgtggct cgccggccac cgccctccgc agcatctctt
    24841 cgcggtcggc aagcccggcg gcgagctcgg atctgtgggg agggatttcc ggcaggacaa
    24901 ataggacctc atcgagggcg agttgccacg ccgcggtttc ggcaagctgc gaatgggcgc
    24961 gcgtcggcgg attgaaggtt ccgctcagca cgccgagccg catcacaggt cctgcatgct
    25021 ggaggatgcc gccgcgtgcg cgcgcgatcc agcgcagctt cggctcatca gcgggcatga
    25081 agcttccggc gaggggggaa gtccccggcg ctctccgcat gcagaatccc gttgcgccgc
    25141 acccggcttc gtcctaccat ctgctcaagc cccttccagg agagagagat gaatgttcga
    25201 gacctgatct ccacccggcg ggaagtctat tcccttcgcg acgacaccag cgtgcatgaa
    25261 gcggcccgct acttgcgtga aaggcagatt cgctcgatcg gcatcgtgga cgccaccggc
    25321 cggctagtcg gggccctgtc gcagagcgac atctccgaca aagtcgcggc cgaaaacaag
    25381 tgcccggctt ggatgaaagt ctccgaaatc atgaccccgg aactggtggt ggtgccgccg
    25441 gacacgccgc tcgacgaatg catccgcctg atggagaaac acggaatttt tcaccttctc
    25501 gtcgtcgatg agaaaggcac ctttcacggc atgatctccg tgcaggacct gctgcgcgtg
    25561 attgcttcgg accacaaggc gagggccgac ctgctggaag ccatgatctt tccggagcgc
    25621 tgaccgcggc gcgggctgcg cccctccgct tcttctctgc ctcagcgcgc agcaggcggt
    25681 gcgctgggct tgtccgagaa ctccgctacg tagacgtccg tcacgcaatc cgtcctgacc
    25741 ttgccatctt cgatggacgg ccactcggga tggctcatgg tggactcaaa cagaaaacga
    25801 ttgccggtga agtccattac catgcgcggc tgagcccagt agtcgcattc gcggcccatc
    25861 aattgggcgc gcgggccgct tgcgctgcga tggtaagcca ccggcgtcaa ccgcacgcgg
    25921 gccggctctc cgcccgcgta ctcgagcact ccgcgcacga tcgcttcgtc ggtagggaaa
    25981 aacttttcca gagatggcgc gaaggtgtat cgctccgtcg aggcaccgag gacgtccttc
    26041 gcgccggcgt ggcgcgcaat gtgctcatac agagtctggc cgggaacatc gcaaccccag
    26101 atgagcacgc gcttcccggt ctcatcatcg tataagccct cttccgactg ccagggagga
    26161 gagagatccc cgttgggctt cctgcagccg atctgtccga cgccgccgcg ggggagcgtg
    26221 tcgccccgta cgcgcgtcag atagcagcgg ccattcgatc cgcaaaaata accggtatgg
    26281 ccatggttgt catcgatctg cacgcgtgcg gacaggtccg cgttatagat gaaattgggg
    26341 gcatcggtgt aaatgacgac cagccggcct ttaggattct gcgtccaatg tgcttcgtcg
    26401 aaacccgcag gagcccggtc gcccggcacg ggcacataca gtgcatgcgc cttgccggtt
    26461 gccggatcaa aaacaccaat gccaatgact ccacttttgg gggtgcaacc cggatcggag
    26521 tgccggcaca gcaagtggac catcagccga tcgcccggtc ccacagacag tgcgtctaga
    26581 ccggtgatag ggaccttctg cccgttagga aggacaaagc cctccatgcg ctgtaggaag
    26641 ttcaccactc cgcgcgtctt gtgagcgatg gggtcgagct ccaacaccat tccgttggcg
    26701 cgcgcataca gcacctcgcg ctcctggctc cactgtacgt ctgccaggcc ggtgccggag
    26761 cccggcacat gcgccgctgc gatcgcatcg tcgtactcca aacggaaatc cggtccgtag
    26821 acgggaaaaa tcctgggcgg atagcgtgcc tgcagacagt cgaagaccat tcttccctgc
    26881 gggctgaact gatttgtata gctgtagaaa tgcgttgcgc cgctgggaca cagattcctg
    26941 tctgacaggc gatagacttt gttcccggtg atgggatcga ggaaaaaccc cgcggtcaga
    27001 cccttcttat gcgctgccgc gggaagtttg acggtgttgt gtacctgcgg ctcggcatgg
    27061 gtttttcttt gggtggtcac cggcgaggcg agaacaaaaa tgattccctg caggaacaga
    27121 attcgtgtga tcgtgaaagt cttgggcaat cagagcctcc gcacgcggag acgcaagcta
    27181 ccgtcaaccg cccctggtgg taaccgccag gaccatcatc actgcgccga ctgccgcggg
    27241 aaacgccgtg tcggtggtta tccctgcacg cgcatacgac tgcgacgcct gccagaacat
    27301 caggattccg ctgccgccaa atgcgattcg tctgatccag cgggccatca tgcgtggcat
    27361 ccagtatagc gccacgaata gagcaggtct agtttgcgga cacgcaaggg tcctccggcg
    27421 cgagcggaaa gatttccttt cgcggctccg gcgcgacgat ttcaacgccg ctgaggtttg
    27481 ctatactgcg ggaacggttg aatcgtcaac tggagttcga ctttcaactc aggactgttc
    27541 atccggcttc atggccactt ttcacatcga gaattttgga tgtcgggcga cgcaggccga
    27601 tgccgcagcc atcgagcggc aactcctgga tcgcggctgt gcgcgatccg ccgacgccgc
    27661 ccatgccgat gtggtcgtcg tgaacacctg tacggtcacc gccgccgccg acgctcaggc
    27721 acgtgacgcc gtgcggcgca tccatcgcgc gagcccctcc gcgcgcatcc tggccactgg
    27781 ctgctatgcc cagcgcgcac cggaagagct ggcggctctg ccgggagtgg aatgggtggt
    27841 ggggaattcg catcagacgg agatttcgcg cgtcattctt ccgtctggat ctcagccggg
    27901 gccgcaaccg gggaacgcgc acgctgaatc ggaacacttc tttccgcttg cgcagctcga
    27961 cggcgttcct gcctcgcttg aacgcggccc ggcgaaaatt ctcaccggag atattttcgc
    28021 gctggccgat cttgcgccgg aaaatatcca gggcgggccc attctcggcg gagaaggcgc
    28081 acgcacgcgc cccatcctga agattcagga cggctgcaat aatcgctgcg cctactgcgt
    28141 gattcccttt gtgcgcgggc gcagccggag cctggcgccg gcgcgtgtgg tggacgagat
    28201 tcgccggctc accgctgcgg gcgcgaaaga agtggtgctc agcggaatca atctcggtag
    28261 ctacgggcgc gacctcacac cgcgcgcgga gttcggcgac ctgctgcgcc gcatcgtgga
    28321 cgaaacgccg gtcgaacggc tgcgcctgag ctcgatcgag cccatggacg tgacacagga
    28381 ttttatcgag ctgctcgcat catcgggccg aatcgcgccg cacttccaca tgcccttgca
    28441 gtccgcctcc gaccgcatcc tcgccgctat gcatcgctgg tatcgcgcgg cgcactacgc
    28501 acggcgcatg gagctgattg cagaacgcct tcccgacgcg gccatcggcg ccgacgtgat
    28561 tgccggattc cccggcgaga cagaggaaga ccatcggcgc acgctcgatt tcatcaccgc
    28621 gctgccattc acctatcttc atccgtttgc gttttcgaag cggccgggaa ctgcaggcgc
    28681 cgcgcttgct gacgaagtgc cgctggccac gattcgacgc cggagcaagg agctgcgcgc
    28741 gctcggcgcc gggaaagcag cagcgtttcg agccggacag gcgggcaaga cgcaacgcgt
    28801 cctcacgctc gaccggagtg gaggggatgc gggcggagat cgctggaccg gcgcactcag
    28861 cgggaattat ctaaacgtgc gggttgccgg ccgctggccg gccaacacct ggctcgaggt
    28921 acgcctcacc gccgcgggtg agcacctcct cggcgcacag cttgctgcgg aagaaaaaca
    28981 aaagatgtaa cacagagagc gcagagacac ggagtccaca gagaggagag agtctctttt
    29041 ttgcagttct cttctatttg ttctccgtgc cgcggtgccc tctgggttat tccgactctt
    29101 ttctgcttcc gcgtgtcggc ggcgtggctg tcttgcaacc gctcaattct cgaaaatcac
    29161 ctcagcgaag gcagtatttc cgctatgagt aatgctgagg gagatgtttt tcacgccgag
    29221 tccggcggcg atctgtcccg cgcgtccggc gacttcaagc gagggtttgc cgctgggaag
    29281 ccgcacgact tcaatgtcta cccagcgcac tcccttcccc cagcctgtgc cgagcgcctt
    29341 catggccgct tctttcgcgg caaaccgccc ggcgtagcgc tccgccttgt tgcgatggcg
    29401 ctcgcaatag gcgatttcgc gggacgtgaa gatgcgctcg agaaaatgcc gcccatgacg
    29461 gcggatggac gcctcgatgc gatcgatctc cgcgatgtcg attcccaatc cgacgatcat
    29521 ggtttagtga cccagccgga ttatactgtt gatttgaaaa acaggaagaa atccgcacgg
    29581 aaacattcag gccgggagaa acgctttgcc ggctctgcgc cggtccgcag ccaacccggc
    29641 tgggggctcg cacgatcgcg cggcatcccc actctgcacg cgaaggcgct tgcgcgtatc
    29701 ccctggctga tccatggatt cagcacgcga cagtgcggcg cgagccggct cggcggcgag
    29761 cgcgtgctga atctcggcaa cacggactgg gacacagaag agaaggtcgg gaagaaccgg
    29821 gcgttgctga tggccgctct tggcgctgaa aaaatgccgc tcgcgtcctt gcagcagtgt
    29881 cacacggata tcctgcacgt cgtcagcaag ccttcggcag aagcgctgcg cggcgacgcc
    29941 ttgctcactg cctcactcgg aatgcttctt gccgtaaaaa ccgcggattg cgtcccgata
    30001 ttgttggccg acaagaaacg ccgcgcgatt gcggcggtcc acgccggctg gcggggcacg
    30061 ctggcgcgca tcgccgagaa gaccgtgggg cggatgcaga tggaattcgg caccaggcca
    30121 agagatgtcg tcgt
//
LOCUS       DASYVO010000371        12836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_111521, whole genome shotgun sequence.
ACCESSION   DASYVO010000371 DASYVO010000000
VERSION     DASYVO010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 12836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12836
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111521"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..661
                     /locus_tag="VGR03_12545"
     CDS             11..661
                     /locus_tag="VGR03_12545"
                     /inference="COORDINATES: protein motif:HMM:NF014663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995150.1"
                     /translation="MSGLGGVEGRTLRKGDVLNIGPASVDGQERTGKNACSTKGTIAS
                     RSTRKRKLSARALQVLQPRKILRVTPGPQSDWFSESAQQIIHAGRYRVAEESNRMGLR
                     LEGAAIPAAPGGVWRQETGKNACPTGGAGEMISEGVSLGAIQVPDGGMPIVLFVEQQT
                     TGGYPKIANVISADFHSLGQLRPRDEIRFERVGWEAARALLREQEELMASEELILE"
     gene            658..1440
                     /locus_tag="VGR03_12550"
     CDS             658..1440
                     /locus_tag="VGR03_12550"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEV1995151.1"
                     /translation="MKRGQLVIDLNCDMGELPEVIADGTQETLMRSVTSVNVACGGHA
                     GDERTMKTTIEQALRWKVAIGAHPGYADRANFGRLELKLPLGEIAASVFEQVRALAEI
                     AASCGARLVHVKPHGALYNQAVRDRALAEAIALGVARWSRDVVLVGLAGSPMLDVFRE
                     AGFEVAAEAFADRRYEPDGTLRSRKFKDALIRDPAEAGGQALSIVERGVLIAYDGTEV
                     SVDAQTICIHGDTPGAPQIAATVARTLRDAGVVLSALPHSSI"
     gene            complement(1545..1979)
                     /locus_tag="VGR03_12555"
     CDS             complement(1545..1979)
                     /locus_tag="VGR03_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995152.1"
                     /translation="MIAGILAPVLNAQKGTAPNGYYPQNYSGSIFTGSLDSADANTQE
                     ITLVFSKGNKTERFVGQLEATCGWKDKGGTLHSFGADEIPKGSLLTAFYIPVTKKVDG
                     HKTTQNAVFAISYLEFEGKKLPDEKRAIIFCSTQKSLRFMAF"
     gene            complement(2332..2853)
                     /locus_tag="VGR03_12560"
     CDS             complement(2332..2853)
                     /locus_tag="VGR03_12560"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEV1995153.1"
                     /translation="MDYKIGDKVVYPNHGVGTVEQISYGVLNGRTERYFMIRIVSSGL
                     RVMVPQSNAMTVGLRSVIRSAESTKVLGFLETGKLNSHHDWKHRFKENSERMRTGALL
                     EVAVVLKSLVSLSRSKPLSFREKKMLERAKYLLVSEMATSRNTTAENAEATVVKSLAK
                     AKLQFPVMVEKFE"
     gene            3134..3847
                     /gene="tsaB"
                     /locus_tag="VGR03_12565"
     CDS             3134..3847
                     /gene="tsaB"
                     /locus_tag="VGR03_12565"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV1995154.1"
                     /translation="MIVLAIDTCDSRGSVATLRDEAVLKVVAHNTQEDYSTWLLPAVG
                     ECLVGSGLQMEDVDAYAAAAGPGSFTGVRVALATVKAWAEVYGKRIAAVSRLEALAAE
                     VSGSAPWVAAFAGANRGEVFGAVYRRNGTSLVRMVDEMVIAPGKFVEAAVECAGSDGI
                     SWVSTDAECVVSEEAWRKREVRGERVERVSSIRAPMIGRLGLAAITEGRFTDVLALDA
                     NYVRRPDAEVFWKGAAHGR"
     gene            3837..4313
                     /gene="rimI"
                     /locus_tag="VGR03_12570"
     CDS             3837..4313
                     /gene="rimI"
                     /locus_tag="VGR03_12570"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV1995155.1"
                     /translation="MAAETKREVAAATIRAFREADAGAVGALAQEASELANWTEENYR
                     ESLSWQGIVALVREDEGKVRGFIIGRQVADEGEIYNLAVGAASRRRGEGGTLLKAAME
                     EFRARGVGRVFLDVRESNAAGIAFYEKHGFSKTGRRAGYYRDPEEAAIVMEMKLTG"
     gene            4416..4658
                     /locus_tag="VGR03_12575"
     CDS             4416..4658
                     /locus_tag="VGR03_12575"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEV1995156.1"
                     /translation="MASAQRTPRDIPFETDAEYQRLAEQHRQYEAELHKISKSPYLNS
                     EDLLEEIKLKKMKLHCKDEMERIAARIMRTHSNYTQ"
     gene            4689..5369
                     /locus_tag="VGR03_12580"
     CDS             4689..5369
                     /locus_tag="VGR03_12580"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HEV1995157.1"
                     /translation="MTMRARGENAERGNAGSTPGSMVKEGYYFGVPPLVLGGVGFLLH
                     WYVAAAVLVLLALFVFSFFRDPERVIPAEPGAVVSPGDGRVVVVTEEENAGRPGKRVS
                     IFLAVWNVHVNRSPAAGTITKMEYRPGKFLAAMRERASVENEQNVFTLSTDAGEMVFK
                     QIAGLIARRVVSWKKAGEKVARGERIGLVRFGSRVDMWVPKEAEILVKVGENVKGGSS
                     VLARWPAK"
     gene            5436..6263
                     /gene="pssA"
                     /locus_tag="VGR03_12585"
     CDS             5436..6263
                     /gene="pssA"
                     /locus_tag="VGR03_12585"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEV1995158.1"
                     /translation="MPDPEQHEIPFQERRIKNPKLRRGIFLLPAAFTSANLLCGYYAI
                     VASLVGTPDDFNHAAKAIGFAILFDSVDGRVARMTGTNTEFGVQFDSLADVVSFGVAP
                     AILAYSWGIRSVNGMSAEALHQLGLFGWVCCLFFLICCAWRLARFNVKGMAPGGSKYF
                     VGMPTPAAAGVIASLVHAFHRAGPLQDFRWSVGWLLMAAALGGLMVSTIRYYSFKDLP
                     WTRKQPAFVILFLILFGGVVYAFSEYVLVLLAGTYAVVGVLLHLVRFIRHRLISRTP"
     gene            6267..7274
                     /locus_tag="VGR03_12590"
     CDS             6267..7274
                     /locus_tag="VGR03_12590"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HEV1995159.1"
                     /translation="MPSFERDSNRIVIAGASSLLGAELKSLLEESRFAGWDLRLVDEE
                     VAAGTLTEAGGEPAVIQPVEEGSFDRARYVFFAGSFEFTLANFQLAQNSGAVIIDLSG
                     HSAAFANSFAWFPGLEKLRGRALPEKPTLASIPSAAAEAIVRLSLSLQDLGLRHLTSV
                     VFQPVSAAGKRGVEELETQTSQLLSFESAGKPLFDAQVAFNILDRFGPASRVDLRQAL
                     AILRTDVHSCLRNTAVVPAIQLVHVPVFYGITFSACAELDPAVDAARIVSACEAGAFS
                     ILQPPETPSNVGVAGESVIQLGQAEGDPVKPGTWWFWGAADNIRLPAANAVKLAEKLA
                     E"
     gene            7271..7825
                     /gene="lptE"
                     /locus_tag="VGR03_12595"
     CDS             7271..7825
                     /gene="lptE"
                     /locus_tag="VGR03_12595"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEV1995160.1"
                     /translation="MKLTWGKTVVMGMSVVLSLAAVGCGYHVAGRSDSLPKSIHVIAV
                     PALENKTTSYRIEQRLTTATVHEFLAKTSYRVVPDPANGDAVLRGKVLSVEAVPLLFD
                     TATGRATTMLVTLKCEITFEERETGKILYHTDSFVFRNQYEISTDANNPNSIKNFFEE
                     QDPALDRMAQDFAARLVAAVVENY"
     gene            7825..8880
                     /gene="holA"
                     /locus_tag="VGR03_12600"
     CDS             7825..8880
                     /gene="holA"
                     /locus_tag="VGR03_12600"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1995161.1"
                     /translation="MARISPEELVARLEKGKPVPAILLLGEEPYLRDDCRKLLIERFV
                     PEASRTWAVSRYSAGRGETQAALDQAQTMAMLSPQQVVFLEDVEAIEKLGEKNRDEAV
                     AQLGAYLEDPAPFTVLVMEATGLDQRMKLGKLLAEKTLVVECSLGEKVEDRLASATSL
                     AKALAKEKGVEFEKGAAEDLAEFVAADLMRLKTEIEKLVTYTAEKKVIGRADVSALVI
                     SEKTTTVWELADMLASRQSKLAMEFLDRLLREGEQPLQLLGGIAWMYRKLIEASELKG
                     VANGWQAARALGMRPEQAELALQNARKISKPRLLAGLHALRNADDRLKGSGTEPRTVM
                     EFLVSELTGGEANAARG"
     gene            complement(9050..10108)
                     /locus_tag="VGR03_12605"
     CDS             complement(9050..10108)
                     /locus_tag="VGR03_12605"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV1995162.1"
                     /translation="MPTPIPITRVSTSRLTEALRENSEFGATFSDHMLVADWEAGSWK
                     QPQIVPFGPISFSPALTPFHYGQAIFEGFKAHRISNGGVALFRPRENFARLNRSAARL
                     AMPGIPEPLFLEGVTELIRLDRDWVPHREGGALYVRPTYFGVDDTLLVRPANRFRLIV
                     MTCPVGPYFAQPIRLLAEERFVRAFPGGTGDSKAAGNYAGGLLAARLAQEKGFHNVLW
                     LDGIERRYVEESGVMNVFFVLDGKAITPPLSGTILPGVTRDSALTLLSEMDIPAEERQ
                     ISIDEVLSAHAAGKLTEAFGAGTAAIVAPIACIRYRDRDLQFPAVSDSSVASRLRSRL
                     VAIQTGREPDLHNWLSPV"
     gene            complement(10198..10503)
                     /gene="rpsT"
                     /locus_tag="VGR03_12610"
     CDS             complement(10198..10503)
                     /gene="rpsT"
                     /locus_tag="VGR03_12610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV1995163.1"
                     /translation="MAQGQATKVKKKKKSVLKRAAQSIQRAEVNRANRTRVRTMMRRL
                     RTAITAGDATAAGNLLQPTMSAIDQAITKNVLHENTANRYKSRLTLAYNGIKAKAAK"
     gene            10802..11128
                     /locus_tag="VGR03_12615"
     CDS             10802..11128
                     /locus_tag="VGR03_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995164.1"
                     /translation="MLRIKNFDGTIRDIQGADFDAAKASDPLLKVVTMEPLPKGSQYV
                     TCEDMVLKKAIDADFLAMDEENKMLNRAFNSICQRSDIGNAAKAACLDFSREYLRLLS
                     RHLPEK"
     gene            11544..11879
                     /locus_tag="VGR03_12620"
     CDS             11544..11879
                     /locus_tag="VGR03_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995165.1"
                     /translation="MQKSVEGASAQLHEWNDKDQLLCLMTLVRPRQRAIQVENDDCGE
                     KFARGKLRLMGRIKHISSDELTFETEKGPKSAFVVLHESTFKTFSGKLVVVSRYGDRF
                     VFCPVPKSN"
     gene            complement(11992..12708)
                     /locus_tag="VGR03_12625"
     CDS             complement(11992..12708)
                     /locus_tag="VGR03_12625"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEV1995166.1"
                     /translation="MQCPGCGTEMTAMTLDGRMGGQVAIDVCARCQAFWFDHFESLKL
                     SPGSTLKLMKFIGEHSTPGKPSLPDALHCPRCATTLHLAHDLQGNMPFTYWRCATDDG
                     HFISFLEFLKEKNFIHPLSRDQINELRQNVQFVNCSNCGASINLESNSACPYCHSPIS
                     MLDMKQPQRMLEQLKQAADPKPLDPMLPMKLASAKLQLETSLADHERGPEWWSDASSS
                     GLVWAGLNVVARWLSDKLVD"
BASE COUNT         2763 a         3065 c         4314 g         2694 t
ORIGIN      
        1 aacgcacatt ttgagtgggc tcggaggggt tgaggggagg acgttgcgga agggggatgt
       61 gctcaacatt gggccggcga gtgtggatgg gcaagagaga acaggcaaga atgcctgttc
      121 caccaaaggc accattgcat cgaggtccac ccgcaaacga aagctgtcgg cgcgggcgtt
      181 gcaggtgctg cagcctcgaa agattttgcg tgttacgcct ggcccacaga gtgattggtt
      241 ttcggagtca gcacagcaaa ttatccatgc gggcaggtat cgtgttgcag aagagtcgaa
      301 tcgcatgggg cttcgactgg agggtgcggc gattccggcg gctcccggtg gtgtgtggcg
      361 gcaggagaca ggcaagaatg cctgtcccac tggaggcgct ggagaaatga tttccgaggg
      421 tgtttcgctc ggcgcgattc aggtcccaga cggaggcatg cccattgttc tttttgtgga
      481 gcagcagacc acggggggat atccgaagat cgccaatgtg atttcggcgg attttcacag
      541 tttggggcag cttcggccgc gggatgagat tcggtttgaa cgagtggggt gggaagcggc
      601 gcgagcgctg ttgagagaac aggaagagct gatggcttcc gaggagctga ttttggaatg
      661 aaacgcggtc agctagtgat tgatttgaat tgcgacatgg gcgaattgcc ggaggtgatc
      721 gccgatggca cgcaggaaac gctgatgcgc tcggtgactt cggtaaacgt ggcctgcggc
      781 gggcatgcgg gcgacgaacg gaccatgaag acaacgattg agcaggcgct tcggtggaag
      841 gtggcgatcg gggcgcatcc tggatacgcg gatcgagcga atttcgggcg gctggagttg
      901 aagctgccac ttggtgagat tgcagcttcc gttttcgaac aagtgcgggc gctggcggag
      961 attgctgcga gctgcggagc gcgcctggtt cacgtcaagc cgcatggggc gctttacaat
     1021 caggcggtgc gtgatcgcgc gcttgcggag gcgattgcgc ttggcgtcgc tcgatggagc
     1081 cgcgatgtcg tgctggtggg cttggcaggg tcgccgatgc tggatgtgtt tcgggaagcg
     1141 ggctttgaag tcgctgccga agcgttcgcc gaccggcgct acgaaccgga tggaactttg
     1201 cgctcgcgca agttcaagga cgcgctgatc cgcgatcctg cggaagctgg ggggcaggcg
     1261 ttgagcatcg ttgagcgagg ggtgctaatt gcctacgacg ggacggaagt ctccgttgac
     1321 gcgcagacaa tttgtattca cggcgacact cccggcgctc cgcaaatcgc cgcaacagta
     1381 gctcgtactc tccgcgatgc cggagttgtg ctcagcgcgc tcccccactc ctcgatttaa
     1441 agagtccaag cgaaacaggc ggtggaggca ttggtgcaac ctctcccaca cccaagtccg
     1501 actgcttact gtaagaggtc aagggttgcg gctccgaggc gttcctagaa ggccatgaag
     1561 cgcaaagatt tctgcgtgga acagaaaatg atcgcgcgct tttcgtcggg aagtttctta
     1621 ccttcaaact cgagataact aatggcaaaa acagcgttct gagtcgtttt gtgtccatct
     1681 actttcttgg tcacgggaat ataaaaagcg gtcaggagcg agcctttggg gatttcatcg
     1741 gcaccgaaag aatgtagcgt tccacctttg tctttccagc cacaggttgc ttccagctgg
     1801 ccgacgaatc gctccgtctt gttgcctttt gaaaaaacta gcgtaatttc ctgagtgttt
     1861 gcatccgccg aatcgaggct cccggtgaaa atcgaccctg aatagttttg cggatagtag
     1921 ccgtttggtg cagtaccttt ctgtgcgttc aaaactggcg cgagaatgcc cgcgatcaag
     1981 attcgggcca agaaaaggcc tcccctgagc gcccaaacgg ggaggacggt cgcaaacgtc
     2041 gcgaggagat tattagtttt ggtcattttc ggtcgttttc aaagccgttt caaggcatct
     2101 tcagggcggc gatttcaagt ctaatgccaa gagcatcaaa agcaagcaat cttataccat
     2161 gttccggaac cagcacgcgt aaacgacgga aagaaagaaa aacgccagga acaatggtca
     2221 cacgctgcta gatgggaagt atcttcaaaa aaaggggcgc agcggagaca aaaagaagcg
     2281 ggcagctaaa gctgcccgcc ggggaaggaa gtgccgcgga gacggcaagg gctattcgaa
     2341 tttttcgacc attacgggga actggagctt ggccttggcg agggacttta cgacggtggc
     2401 ctcggcgttt tcggcggtgg tgttgcgaga agtggccatc tcgctgacga ggagatactt
     2461 cgcgcgttcg agcattttct tttcgcggaa ggagagaggc ttgctgcggc tgagggaaac
     2521 gagactcttg aggacgacgg cgacttcgag gagggccccg gtgcgcatgc gctcggagtt
     2581 ctctttgaag cggtgcttcc agtcgtgatg agagttgagc ttgccggttt cgaggaagcc
     2641 aaggactttg gtggattccg cggaacgaat aacggaacga aggcccacgg tcatggcgtt
     2701 tgattgcggg accatgacac gcaagccgct ggaaacaatg cggatcatga agtaacgttc
     2761 ggttctgccg ttcaaaactc cgtagctgat ctgctcaaca gtgcccacac cgtgattcgg
     2821 gtaaacgact ttgtcgccga tcttgtagtc catagcttcg gttgcccccc agagatgcat
     2881 aaggaatata taatagtgtg ctggaagagt caaggtatat cccgtggaat caggaggtta
     2941 gggaaagtta ggtactaatt tggcaataga attgctcggc ggggtgggtt tttgtggtgc
     3001 tgaacaatgc tattaggagc agttcaaggc cgaaaatcga ccgattgctg ggggattccg
     3061 gtttggatca cttcgagcga cccgcctcgg aaggcgggtc ctacagcgtg ggggagtacg
     3121 gtagaatgcg gatgtgattg tactggcgat agatacgtgt gattcgaggg gcagcgtggc
     3181 gacgctgcgg gatgaggctg tgttgaaggt tgttgcccac aatacccagg aggactattc
     3241 cacgtggctg ctcccggcgg ttggtgaatg cctggtgggt agcgggcttc agatggagga
     3301 tgtggacgcg tacgcagcag cggccgggcc ggggtcgttt accggggtgc gggtggcgct
     3361 ggctacggtg aaggcttggg cggaggttta cgggaaacgg atcgcggcgg tctcgcggtt
     3421 ggaggcgctg gcggcggaag tctcggggag cgcgccctgg gtggcggcgt ttgcgggcgc
     3481 gaaccgcgga gaagtatttg gggccgttta tcggcggaat gggacgtcat tagtgcggat
     3541 ggttgacgag atggtgatcg ctccggggaa atttgtggaa gcagcagtgg aatgtgcggg
     3601 tagcgacggc atttcgtggg tttcgacgga cgcggaatgc gttgtttccg aagaggcgtg
     3661 gagaaaacga gaggtgcgcg gtgaaagagt tgagcgtgtt tcaagcatcc gcgctccgat
     3721 gatcggtcgt cttggactgg cagcaataac tgaggggcgg ttcacggacg tgctggcgct
     3781 ggatgccaat tacgtgcggc ggccagacgc ggaggtattc tggaagggcg cggcgcatgg
     3841 ccgctgagac gaagcgcgag gtcgctgcgg cgacgattcg cgcgtttcgc gaagcggacg
     3901 caggcgcggt gggggcactt gcacaggaag cttcggaatt ggccaactgg acggaagaaa
     3961 actatcggga gtcgctgagt tggcagggga tagtggcatt ggtgcgagag gacgagggga
     4021 aagtcagggg atttattatc gggcggcagg tggcggacga gggggagatc tataatctag
     4081 ccgtcggggc agcttcgcgg cgccggggag agggcgggac actgctgaag gcggcgatgg
     4141 aggagtttcg agcgcgcgga gtaggccggg tatttctaga tgtgcgcgaa tcgaatgcgg
     4201 ccggaattgc gttttatgag aagcacggct tttccaaaac ggggcggcgc gcgggctatt
     4261 accgcgatcc ggaggaggcc gcgattgtga tggagatgaa actgacgggc taggacgaaa
     4321 ttcccacagg ttcctacttg acgtggcgaa ccagagtgct atcttgcgtt tatgtagtgc
     4381 tcacatccca atcacgaaaa ggtcaggagg aacgcatggc aagcgcgcag cggacacctc
     4441 gggacatacc cttcgagaca gacgccgagt atcagcgtct ggcagagcaa caccggcaat
     4501 acgaagcaga actgcacaaa atctccaagt cgccctacct gaattcggaa gacctcctgg
     4561 aagaaatcaa actgaagaag atgaagctcc actgcaagga tgagatggag cggattgccg
     4621 cgcgtatcat gcgcacgcat tccaattaca cccaataacg actttatcgc aggcgatttt
     4681 gtcgtagaat gaccatgcgc gctcgcggcg agaatgccga gcgcggaaac gctgggtcta
     4741 ctccgggaag catggtcaaa gaaggctact actttggggt gccgccgctg gtgctgggcg
     4801 gcgtcggttt cttgctgcat tggtatgtgg cagctgctgt tctggtgttg cttgccttgt
     4861 ttgttttctc tttctttcgc gatccggagc gcgtgattcc ggcggaacct ggggcggtgg
     4921 tgtctccggg cgacggacgc gtggtggtgg tcacggagga agagaacgcg ggaaggccgg
     4981 gaaagcgggt gagcattttt ctggcggtgt ggaacgtgca tgtgaaccgg tcgccggccg
     5041 caggaacgat tacgaagatg gaataccggc cggggaagtt tctcgcggcg atgcgggaac
     5101 gggcttcggt ggaaaacgaa caaaatgtgt tcacgctctc gacggatgcc ggagagatgg
     5161 tgttcaagca gattgcggga ctgattgcgc ggagagttgt ctcctggaag aaggccggcg
     5221 aaaaggtggc tcgcggagag cgcattggac tggtacgatt cgggtcgcgg gtggacatgt
     5281 gggtcccgaa agaggcggag atcctggtga aggtggggga gaacgtgaag ggcggatcga
     5341 gcgtgctcgc gcggtggcct gcgaaataaa ttgtatggat ttcgtatggc agagatggac
     5401 gcaaagctaa cagcgacggg gaagcgaacg taaccatgcc agatccagaa caacacgaga
     5461 taccgtttca ggagcggcgc atcaagaatc cgaaattgcg gcgggggatt tttctgcttc
     5521 ccgcggcgtt cacgtcggcg aatctcctgt gcggttacta cgctatcgtc gcgtccctgg
     5581 tcgggacgcc cgatgacttt aaccatgcgg cgaaggcgat tggatttgca attttgtttg
     5641 attctgtgga cggccgagtc gctcgaatga cggggacgaa cacggaattt ggagtgcagt
     5701 tcgactcgct ggcggatgtc gtcagttttg gggtagcgcc tgcgattctc gcgtattcat
     5761 ggggcattcg aagcgtgaac ggcatgagcg cagaggcgct gcaccaactc ggactctttg
     5821 gctgggtgtg ctgcctgttt ttcctcattt gctgcgcttg gcgtctggcg cgatttaacg
     5881 taaagggaat ggcgccggga ggatcgaaat attttgtagg aatgcccact cctgccgccg
     5941 ctggggtgat tgcatcgctc gtgcacgcat tccatcgcgc ggggccgctg caagatttcc
     6001 ggtggtcggt tggctggctc ttgatggcgg cagcgctggg agggctgatg gtcagcacga
     6061 tccgttatta tagttttaag gaccttccct ggacacgaaa gcagccggca ttcgtcatcc
     6121 ttttcttgat cttgtttggc ggagtggttt acgcgttttc ggaatatgtg ctcgtcctgc
     6181 tggctggcac gtatgcggtg gtcggtgtct tgctacatct tgtgcggttc attcgccatc
     6241 gcctgatttc gcggaccccg tgattcatgc cttcgtttga acgggactcc aaccggatcg
     6301 tgattgcggg tgcgtcttcg ctgctgggag cggagctgaa gtcgctgctc gaagagagcc
     6361 ggttcgcggg atgggatttg cggctggtgg atgaggaagt ggccgcgggg acattgacag
     6421 aggcaggcgg ggagccggcg gtgattcagc cggtggaaga aggcagcttc gaccgcgcgc
     6481 gctatgtgtt tttcgcgggc tcgttcgaat tcacgctcgc gaatttccaa ctggctcaga
     6541 acagcggggc tgtgattatc gacctgtccg gccactctgc ggcgtttgcc aactctttcg
     6601 cttggtttcc gggcctggag aaacttcgcg gccgagctct tcctgagaag cccactcttg
     6661 cctccattcc atccgctgct gccgaagcga tcgtgcggct ttcgctctcc ttgcaagacc
     6721 tcggcttaag gcatcttacg tccgtcgtgt tccagcctgt ttccgcggca ggaaaacgag
     6781 gcgtggaaga actggaaacg cagacaagcc agttgctttc atttgagagc gcgggaaagc
     6841 cgcttttcga tgcgcaggta gcgttcaata tcctggatcg ttttgggccc gccagccgag
     6901 tcgatctgcg ccaagcgttg gcgatacttc gaacggatgt gcactcctgc ctccgtaaca
     6961 cggcggtggt tccagcgatt cagttggtgc atgtgccggt attttacgga atcacgtttt
     7021 cggcctgcgc tgagctggac cctgccgtgg atgcagcgag gatcgtcagc gcgtgcgagg
     7081 caggggcgtt ttctattctt caaccgccag agactccgag caatgtgggt gtcgcgggag
     7141 aatcggtcat ccaactgggg caagcggagg gtgatccggt caagccgggg acctggtggt
     7201 tctggggagc ggcggataat attcgattgc ccgcggcgaa tgcggtgaag ctggcggaga
     7261 aactcgcgga atgaaactca cgtggggaaa gacggtggtg atgggaatgt cggtggtgtt
     7321 gtctctggcg gcggtgggct gcggatatca cgtggcgggg cggagcgact cgctgccgaa
     7381 gagcatccat gtgattgcgg tgccagcgct ggaaaacaag acgaccagct accgcatcga
     7441 acagagattg acgaccgcta cggtgcatga attcttggcg aagacctcgt accgagtggt
     7501 gcccgatccg gcgaacggcg atgcggtgct gcgaggtaag gttctgagtg tggaagcggt
     7561 accgctgctg tttgatacgg cgacggggcg cgcgacgacc atgctggtta ccctgaagtg
     7621 cgagatcacg ttcgaagaac gcgagacggg aaagattctc tatcacacgg acagttttgt
     7681 tttccgcaac cagtacgaga tttcgacgga tgcaaacaat ccaaacagca ttaagaattt
     7741 ctttgaagag caagatcccg cgctggaccg catggcgcag gactttgcgg cgcgattggt
     7801 ggcggcggtg gtggagaact attgatggcg cggatttcgc ctgaggaatt ggtggcgcgg
     7861 ctggagaagg ggaaacccgt tccggcaatt ctgcttttgg gggaggagcc gtaccttcgc
     7921 gatgactgtc gaaaactact gatcgaaaga tttgtgccgg aggcgtcgcg gacctgggcg
     7981 gtgtcgcggt attcggcggg gcgcggagag acgcaggcgg cgctggatca ggcgcaaacg
     8041 atggctatgc tttcgccgca gcaagtggtt ttcctcgagg atgtggaagc gatcgagaaa
     8101 ctcggagaga aaaaccggga cgaggccgtg gcgcagctcg gcgcgtatct cgaagatccc
     8161 gcgccgttca cggtgctggt gatggaagcg acgggtctgg atcagcgcat gaaattgggc
     8221 aagctgctgg cggagaaaac gttagtggtg gaatgcagtc ttggcgaaaa ggtggaagac
     8281 cggctggcat cggcgacgag cctggctaaa gcgctagcga aagaaaaggg agtggagttc
     8341 gaaaaaggcg ccgcggagga cctggcagaa tttgtggccg cggatttgat gcggctgaaa
     8401 acagagatcg agaagctggt cacgtacacc gcagagaaaa aagtgatcgg gcgggcggac
     8461 gtgagcgcgc tggtgatttc ggagaaaaca acgacggttt gggaattggc ggacatgctg
     8521 gcgtcgcggc aatctaagct ggcgatggaa tttctggatc ggttactgcg cgagggggaa
     8581 caaccactac agctgcttgg tgggatcgct tggatgtacc gcaaactgat cgaagcgagc
     8641 gagttgaagg gcgtagcgaa tggctggcag gcggcgcgcg cgctggggat gcggccggag
     8701 caggcagaat tagcgttgca aaatgcgcgc aagatttcca aaccgcgcct gctggcggga
     8761 ttgcacgcgc tgcgaaacgc ggatgaccgg ctgaagggca gcggcaccga gccgcggacg
     8821 gtgatggaat ttttggtttc ggagctgact ggcggagaag cgaacgcggc gcgcggctag
     8881 gagaacgaga aaaaagaaat aatacagaga acacagagaa cgcagagttc acagagttca
     8941 cagagaagaa agttcagaga acggcgcggg aaaaatagga aagggcggcc agtgggccgc
     9001 ccgttttgtt gtggaggagg gagaaagacg agatggttag aaatccggct cagacggggg
     9061 agagccaatt gtgcaggtcc ggttcgcggc cggtttggat ggcgacgagg cgggagcgga
     9121 ggcgcgaggc gaccgaggaa tccgaaacgg cagggaattg caggtcgcga tcgcggtagc
     9181 ggatgcaggc gatgggagcg acgatggcgg cggttccggc gccgaaagct tcggtgagtt
     9241 ttcctgcggc gtgggcggag agcacttcgt cgatggagat ttggcgttct tcagcgggga
     9301 tgtccatctc gctaagcaaa gtgagagcgg agtcgcgagt aacgccgggc aggatggtgc
     9361 cgctgagagg cggagtgatg gctttgccgt cgagaacgaa gaagacgttc atgacgccgg
     9421 attcttcaac gtaacggcgc tcgatgccgt cgagccacag cacattgtga aagccttttt
     9481 cctgggcgag gcgcgcggcg aggaggccac cggcgtagtt gccggcggct ttgctgtcgc
     9541 cggtgccgcc ggggaaggcg cgaacgaagc gctcttcggc aagcaggcga atgggctgcg
     9601 caaaataagg gccgacggga caagtcatga caatgaggcg gaaacgattg gcggggcgaa
     9661 cgagaagtgt gtcgtccacg ccgaaatagg tcgggcgaac atagagggca ccgccttcgc
     9721 gatgtgggac ccaatcgcga tccaggcgaa ttaattcggt gacgccctcg aggaagagcg
     9781 gctctggaat tccgggcatg gccaggcgcg cggcggagcg attcaaacgg gcgaaatttt
     9841 cgcggggacg aaagagcgcg acaccgccgt tggagatgcg atgcgccttg aagccctcaa
     9901 aaatggcctg gccataatgg aaaggagtaa gcgcgggaga aaatgagatc gggccgaagg
     9961 gaacgatttg aggctgcttc caggagcccg cttcccaatc ggcgacgagc atgtggtcgc
    10021 taaaggtggc gccgaattcg gaattctcgc gcagcgcttc cgtgagccga gatgttgaaa
    10081 cgcgggtgat ggggatcggt gtgggcatcg ggcctcgcag gtagaaaaag aacgggcgcc
    10141 cgccgcggcg ggcgccccca ctgaaaccgg caaaatcgaa aagcggaaga acccttgtta
    10201 cttcgcggcc ttggctttga tgccgttgta agccagcgtg aggcgcgatt tgtagcggtt
    10261 ggcggtgttc tcgtgcagca cgttcttggt aatggcctgg tcgattgccg acatcgtggg
    10321 ttgcagaaga ttgcccgcag cggtggcgtc gccggcggtg atggcggtgc gcagacggcg
    10381 catcatcgtg cggacccggg tgcgattggc gcgattgact tcggcgcgct gaatagattg
    10441 cgccgcgcgc ttcaaaaccg acttcttctt ctttttaact ttggtagcct gtccctgggc
    10501 catctattac tccttttgcg tatcgctcaa acttctaacg ttaaacgagg ctcacggctt
    10561 tgtcaaacga gccgaaaggt ccgtactgtc ctaatttgcc tgcgaatttg acggggcacg
    10621 ggttacggca tacgcttgcc tcattggagg atgtatgaga ttcaccgtat tccttttgct
    10681 agtcttgacg gctctgtctc tgaacgcaca ggaagattgg cgaacccgag cggagggcac
    10741 tcgtgatcag gcagtccaaa acctcataca cacttactgc ggagataagc ccaggccgcc
    10801 tatgcttcgt atcaaaaact tcgatggcac tatccgtgat atccaaggcg cagattttga
    10861 cgcggccaag gcgagcgatc cgctgttgaa ggtggtcacc atggaaccac tccctaaggg
    10921 atcgcaatat gttacttgcg aggatatggt actcaaaaaa gcgatagacg cggacttttt
    10981 ggcaatggat gaagaaaaca aaatgttgaa cagggccttt aactccattt gtcaacggtc
    11041 cgatatcggc aacgccgcaa aggctgcctg cttagacttt tcgcgcgagt acctccgctt
    11101 actctcgcgc catttgccag agaaatagct caggcacttt tcgtttagag tacacctaag
    11161 gagatcgcgg cgtgcccttg ctgcagacat ccacatgatt actacgtatg tcggagtttt
    11221 ctgcgtccac tgtaaacgct tcatagaact gcgttcttac caagtccagc ggcccgaaat
    11281 caaacatttt gacatttctt cagcgccgga tattcgttgc actcatgagg gctgcaaaaa
    11341 agtttgcgcc taccgcatgg aagacgttgc ctattcaact tcgccagacg gtaaggagcc
    11401 tcagcatccg cataaacgct agcgtcctga cgaagaactc agagccgccg cactccttag
    11461 cactccttct tagtggtggt tttcctgtgt aaatcttgaa agaaagggcg caagtgtagt
    11521 attaggagta gagggggttg caaatgcaaa aatccgtcga aggtgcctcg gcacagctac
    11581 atgaatggaa tgataaggat caactgttgt gtttgatgac tctggtcagg ccccgtcaac
    11641 gcgccatcca agttgaaaac gacgattgtg gtgaaaagtt cgcacgaggg aagctccgtt
    11701 taatgggacg tattaagcac atttcatctg acgagttgac ctttgaaacc gaaaaaggac
    11761 cgaaatctgc atttgttgtc ctgcatgagt ccacattcaa aaccttctct ggaaaactcg
    11821 tggtggtatc ccgatatgga gaccgtttcg ttttttgtcc tgtgcctaaa tcaaattagg
    11881 actctgccca aaggtcggta gtgacacatt ttcaaactga gtcattaccg aatctgctgc
    11941 cttctcggat ggacgcttgg atgctcctaa ttaggggagc gctcatggcg atcaatcgac
    12001 cagcttgtcg ctgagccagc gggcgacgac gttcaggccg gcccacacca ggccggacga
    12061 actggcatcg ctccaccatt ccgggccgcg ctcatgatcg gccaaggagg tttccagttg
    12121 caactttgcg gaggcgagtt tcatggggag catcggatca agcggcttcg ggtcggctgc
    12181 ttgtttgagc tgctcgagca tgcgctgggg ctgcttcata tcgagcatgg agatgggcga
    12241 gtggcagtag ggacaagcag agttggattc caggttgatg gacgcgccac agttggagca
    12301 attcacaaac tgaacgttct gccgcaactc gttgatctgg tcacgcgaca gcggatggat
    12361 gaaattcttt tctttcaaga actcgagaaa actgatgaag tggccgtcat cggtcgcgca
    12421 tcgccaatac gtgaacggca tgtttccttg caagtcgtgc gccaggtgca gcgtcgtggc
    12481 gcaccgcgga cagtgaagcg cgtccggaag cgacggcttt ccgggtgtcg agtgttcgcc
    12541 gatgaatttc atgagcttca gtgtggagcc gggagagagc ttcaggctct cgaaatgatc
    12601 gaaccagaac gcctggcaac gggcgcacac atcgatggcg acctgtccac ccatgcggcc
    12661 gtccagcgtc atcgcagtca tttcggttcc gcagccgggg cactgcatag acaaacattg
    12721 tagctggtcg cctggaccga tgccatcgat cggaaaggaa gtgcaagccg ttcgcgaacg
    12781 agcaacgaca cgggcggtgg cggtggatta gcgcgacccc ttcacgactt gtggaa
//
LOCUS       DASYVO010000372         1982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_264607, whole genome shotgun sequence.
ACCESSION   DASYVO010000372 DASYVO010000000
VERSION     DASYVO010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264607"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VGR03_12630"
     CDS             complement(<1..198)
                     /locus_tag="VGR03_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific methyltransferase EcoRI family
                     protein"
                     /protein_id="HEV1995167.1"
                     /translation="MANDAHDIESKPLNRSMQAAKASKQDEFYTQLSDIEKELKHYAA
                     HFEGKTVLCNCDDPRVSNFFHY"
     gene            complement(391..1383)
                     /locus_tag="VGR03_12635"
     CDS             complement(391..1383)
                     /locus_tag="VGR03_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995168.1"
                     /translation="MKRLCLAVSCFLMIATMGVSARGDEKRTAVVLFPRLSKGQVLRY
                     QISYHRMSRTSTTSMVQDPVSAVNSELSLGLGIRIEVVEITEPGAASADARPAIRLRA
                     AYERAVAMQRTDDPVAEDSAAEKRFAQLEGKSFDCTLDGNGVQECKGTGGDLLPGAAE
                     GMRGWLAQVFAATSLPQKGIAPGESWGDEQEAGEEIPLAGLHWVRQFTFVREEPCLEV
                     PIVLNGEEQRVPLGEEVCAEIRVRNLLVRRGHAKDATPESFRVKGLRTAGSARGANES
                     MIRISMTSGLTVTAGDTGWLTADFTVSTADRERKIRTASELKTDSNLTRVRDLP"
     gene            complement(1380..>1982)
                     /locus_tag="VGR03_12640"
     CDS             complement(1380..>1982)
                     /locus_tag="VGR03_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995169.1"
                     /translation="LPAEEDSAELRAAVGKFCESWKTPRVLHYREENDGLIRRYPEGD
                     TPELQQMGCALIQLWSRHAWRPSAILPAEALRIGKRHEKKSRPGAAGSNPGDSSEMQE
                     WLPAAGGAEVVLHVVQQFSNPDHWVNVGQASGQERRLEKNSFFAESLATLSTLDGSLY
                     DSTRSAACETSRTLDPVEGLPDAPRFSSRLSVTVTIHRIP"
BASE COUNT          365 a          640 c          559 g          418 t
ORIGIN      
        1 gtagtgaaag aaattgctca cgcgcggatc gtcgcagttg cacagaacgg tcttgccctc
       61 gaaatgcgcc gcgtaatgct ttagctcctt ctcgatgtcc gaaagctgag tataaaactc
      121 gtcctgcttg gacgccttcg cggcctgcat gcttcgattg agcggcttgc tttcgatgtc
      181 gtgtgcgtcg tttgccattg acctttggaa gatttcaaac tgcaccacta cccacaactg
      241 ggtatggaat tgggtggtcg aacttccttg catttctcag gacccgagat gaaattcccc
      301 acctacttgt catgcggtaa aggctagcac gtttccttcc atcaagcatg gctccaatct
      361 caaactgcac cactaccaag tttgcgaaag tcagggcaga tcgcgcaccc gggtaaggtt
      421 cgaatccgtc ttgagttcgg aagcggtgcg gatttttctt tctctgtccg ccgtagagac
      481 cgtgaagtcg gccgtcagcc acccggtgtc acccgcggtg acggtgaggc cgctcgtcat
      541 cgagatgcgg atcatcgatt cgtttgctcc gcgagcgctt ccggccgtgc gaagcccttt
      601 cacgcggaat gactcggggg tagcgtcttt ggcatggcct cttcgaacca gcaagttgcg
      661 aacgcgaatc tcggcgcaga cctcctcacc aagcggtact ctttgctctt cgccattcaa
      721 cacgatcgga acttccaggc atggttcttc ccggacaaaa gtgaattgcc tcacccagtg
      781 aagtcccgcc agaggaatct cttccccggc ctcctgctcg tctccccagc tttcgcccgg
      841 agcgattccc ttctgcggca agctggtcgc cgcgaagacc tgcgcgagcc agccgcgcat
      901 gccttccgcc gcgccgggga ggagatcccc tcccgtccct ttgcattcct gcacgccgtt
      961 gccgtccagc gtgcaatcga aggatttccc ttcgagctgc gcgaagcgtt tctcggccgc
     1021 gctatcttcg gcgacggggt catcggtgcg ctgcatggcc acggcccgct cgtaggccgc
     1081 gcgcagtcga atcgcaggac gcgcgtcggc gctggcggct ccgggctctg tgatttctac
     1141 gacttcgatc cggattccga ggcccaacga aagctcgctg ttcacagccg acacgggatc
     1201 ctgaaccatg cttgtcgtgc tggtcctcga catgcggtga taggagatct ggtagcgcaa
     1261 cacctgccct ttcgaaagcc gcgggaacaa aacgacggcc gttcttttct cgtcgccccg
     1321 cgcggacacc cccatcgtcg cgatcataag aaagcaagaa acggcgagac acaaacgttt
     1381 catggaatgc ggtggatcgt caccgtgacc gacaacctgg acgaaaaccg gggcgcgtca
     1441 ggaagtcctt ccaccgggtc aagcgtgcgg gaggtctcgc aagcggccga gcgcgtcgag
     1501 tcgtagaggc tgccgtccag agtggaaagc gtcgcaaggg actcggcgaa gaaactgttt
     1561 ttctcgagcc ttctctcctg ccccgaggcc tgcccgacgt tgacccagtg gtccggattt
     1621 gaaaactgct gcacgacgtg aaggacgacc tccgcgcctc cggccgcggg aagccactcc
     1681 tgcatctccg agctgtctcc gggattcgat cccgccgcgc ctggacgcga ctttttttca
     1741 tggcgcttgc cgatgcgcag cgcctcggcg ggcagaatcg cgctcgggcg ccaggcgtgc
     1801 cgcgaccaca actggatcag cgcgcaaccc atctgctgca gctccggcgt gtctccctcg
     1861 ggatagcgcc gaatcaagcc gtcgttttct tcccggtaat gaagcacgcg cggcgtcttc
     1921 caactctcgc agaatttccc caccgccgcg cgcaattccg ccgaatcttc ttcagccgga
     1981 ag
//
LOCUS       DASYVO010000373         9270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_64720, whole genome shotgun sequence.
ACCESSION   DASYVO010000373 DASYVO010000000
VERSION     DASYVO010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9270
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64720"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..1069
                     /locus_tag="VGR03_12645"
     CDS             224..1069
                     /locus_tag="VGR03_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995170.1"
                     /translation="MGATIVGIFVLAMVTIIVLGFRIGWSAVLDWAVVAIPTIAGFAA
                     WVIPVKETRTQHKWVLFVGGLAFSGLIYLQQYTTRRVHSAELANLPTKADIAKLPTAQ
                     DIVKEWRKVQTEEVRKEPGVPIKTAHKSSARQNQDTSLGLGLSKQLDDIKSMLAGQRW
                     GLNAEQLVSLARRMTPYAPEKERGDLITCILGDPDSIKFATSLVAALRSAGWKLPGSG
                     FGQAVFSGDVEGVVIKLNSTADKPPGLFDFVTSLREAGINPVGLVDPKVPPNEFQIII
                     GRKPG"
     gene            1687..2136
                     /locus_tag="VGR03_12650"
     CDS             1687..2136
                     /locus_tag="VGR03_12650"
                     /inference="COORDINATES: protein motif:HMM:NF041379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYCXC family (seleno)protein"
                     /protein_id="HEV1995171.1"
                     /translation="MKVLIGLLIIGCATALAQVSGTQSDNPVPAYHSTPPGGGALIAP
                     ILTPKQLAAGGFTQPVQTESYKAAARVPGVLYQLPCYCFCDRAHGHTSLRSCFESTHG
                     ANCGTCMAEALYAYQRSKKGWTARMIRDGIVHGDWKLIDLQHPAPVH"
     gene            complement(2256..2717)
                     /locus_tag="VGR03_12655"
     CDS             complement(2256..2717)
                     /locus_tag="VGR03_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995172.1"
                     /translation="MLHLRLKEGAPDEPDTSDYDAICSTPSPEAFFGLLFREVAGFSL
                     PAAVITSETEDDIRRTQTGCCGSPALGVEITVVTCTETSDFPVYCYWETHGLPGMTVK
                     WDYALEHAGQLGHTAFRHTRLECTFDTPANQQRFAEIWRRVIGKDPVFEPI"
     gene            3010..4086
                     /locus_tag="VGR03_12660"
     CDS             3010..4086
                     /locus_tag="VGR03_12660"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV1995173.1"
                     /translation="MGMRQGYGKSHASLRVWFWRAWRFFYTRARELRMIVKALLSTGH
                     PVLVHIIPIRRCNLACTYCNEFDDFSDPVPLEEMKRRLDLLADMGTSVITISGGEPLL
                     HPELDEIIRHIRRRGVIAGMITNGFLLSEERIEQLNAAGLEHLQISIDNTVPDEVSKK
                     SLKTLDQKLELLANYAYFQVNINSVLGSGVKDPEDALRIAHRAVDLGLTSTVGIIHDQ
                     NGQFKPLGPREIEIFEEIMSLGKRSYSRFNAFQHQVARGKEHEWRCRSGSRYLYVCED
                     GLVHWCSQQRGYPGIPLAAYTPEIRRREFNTSKPCAPGCTISCSQQVAILDNWRAPQT
                     LKPMPMTPPATPQHDLVQLSSPQR"
     gene            4568..6181
                     /locus_tag="VGR03_12665"
     CDS             4568..6181
                     /locus_tag="VGR03_12665"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEV1995174.1"
                     /translation="MRSMKGCLLFASFLLLSAPFSRAQSGESPGKGDSASSSGATKQE
                     VDQLRKELAAQRQTIEQLKAMLQRLVETKAQPAEGARLVNANLDVTAQMQTPNQEKEQ
                     PQAATSKILRIPGNELKFSVGGGEVQIYGHADVSFDYVDNGLANRFGAVGNNGWLAQV
                     SSNLSYFGVRGSRRLVPHLNGVFQIETEVAFSSTPGPTTDAQVKQGFGSRDTFVGLQG
                     DWGAVKLGKEDAPYKRSTARMDPFINSIGDSRSIMGNSGGDNRSEFNGRQPHSVWYES
                     PKFKGFYASVLFSPGQNRSKDNTILARVEPNCAGGNDAPCTDGAFGNVLSAVVSYTYG
                     SLYAFTAYEHHAKANRTGDELASIADPSIPPAGSVGIADESAWKLGAQYEFKLTRTTA
                     NFVYEKLKRYAPAVPAFNERSRPNATWLAIVQKMTPNDDLNFGWAHAGKTPGDPGGQI
                     NQPGTVIGIAGPIDNRSNLIDVGYKHHFSDKRTSAYFVFATQMNHQGAHYDLGANGHG
                     AVVDRADAAGNSFTGLTHKGLSVGLNYDF"
     gene            complement(6552..7817)
                     /locus_tag="VGR03_12670"
     CDS             complement(6552..7817)
                     /locus_tag="VGR03_12670"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HEV1995175.1"
                     /translation="MMRRSLILCLLAGVVVAVALAADLPDAWHSWRYSRTLPAGEPGS
                     RYELLLPEDVFFHSENHLADLRVIDDRGAEVPFVLITDRPSVQTESRSVTLRERSHVP
                     GQYSQVILDLGLHAAFHNRVRVETPESDFILWVEVAVSDDARTWRIVNARAPISRFRK
                     ETLQGNQTIRYSDTNARYLRLRIFEKSHEFPVTAASVAFSTGAREAIRARLSADLTPD
                     AARPATLSRWSADLGTDQIPVNEVVFETSQPEFFRAVRILTSEDSKEWRFAAGGEIYR
                     FHVGEKLEESLRVAVSEYWRARYWRVEVVNGNDAPLENVRPVLARYSVGILIIPEPGR
                     SYRLLYGNARAGTPEYDLGRTLPSAVTRTLAPRNAALGPEETTSNYADPRPFTERHPA
                     LLWVVLGVAIILLGYASLRSLGAAPANRT"
     gene            complement(7814..>9270)
                     /locus_tag="VGR03_12675"
     CDS             complement(7814..>9270)
                     /locus_tag="VGR03_12675"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEV1995176.1"
                     /translation="FDNNWIGPRGRVGIGILLGSAMLPWSQWLLARGYTYFSEGIAGL
                     GEAVLYVSIWAGWHYYALFSQTTAFAGMIVVTAVMAAVAIGRDSQRIALLSLLGGLLT
                     PIIVSTGRDQQVVLFTYLLILGAGMLFIAARRDWRYLAPVAFVLTQAYFWGWYGSFYR
                     SEVLARTVFFATLFFVLYAAVPVVRAARHGSLDWLDPILVLLNAGIYLLALRVMLWDE
                     HRWALTVAVLLLSATHLFVARMAPAPKAGESPWTRILFAGLALTFATLAIPIRLEGKW
                     ITLAWAIEGAILIWSGLRALLPLLRHAGYFLLSVAALRLLLLPIPAPQFLLNARFAAF
                     AVVIACFAVSVVAARKQWSSLGRNERQAYGVLAVAINGFTLVALSLELWDYYGRAGAT
                     ALDRDLSQHLALSVLWTCYATALILLGVKRQSALLRWQALALFGLVVGKVFIYDSSYL
                     ERFYRILSFLILGLLLVVVSFLYQRKLVCRQTRP"
BASE COUNT         1998 a         2604 c         2958 g         1710 t
ORIGIN      
        1 caccactaca agaatttcag gccgggtaat ggggcagttt ccgtgcgcgt gggcacgccc
       61 gggggccccc tgtcttctga aacgatctga ttccaaatgg ttttaggcag gattatatac
      121 ctcacccaat gaacgagtta aaatgtctag catgcattca caagcccgtg tgcctctttg
      181 gaaggcaggt gttttgcagg ctcaacggag aagacgaatc cgcgtgggcg ctacaattgt
      241 cggcatattc gtattggcga tggttacgat cattgtgctt ggctttagga ttggctggag
      301 tgctgttctc gattgggcgg ttgttgcaat ccccactatc gctggctttg ccgcatgggt
      361 gattccagtg aaggagacga gaacacagca caaatgggtt ctttttgttg gcgggctggc
      421 gttcagcgga ttgatttacc tgcaacaata cactacccgc agagtacatt ccgcagaact
      481 ggcgaacctg ccgaccaagg cggatatagc taaacttccc actgctcaag acatcgtaaa
      541 ggaatggcgc aaagtacaaa cggaagaagt aagaaaggag cccggtgtac cgatcaagac
      601 tgcacataag tcatctgccc gacagaatca agacacctca ctggggctcg ggttaagcaa
      661 acaattagac gacatcaagt ctatgcttgc tggacagcgc tggggcctaa acgcagagca
      721 gttagtttca cttgcacggc gaatgactcc atatgctcca gagaaagagc ggggagactt
      781 aatcacgtgt atattaggcg atcctgatag cataaagttt gctacttctc tcgtagcggc
      841 gttgcgttct gctggatgga agcttcccgg aagtgggttt ggtcaggctg tattctctgg
      901 agacgtcgag ggcgtcgtta tcaaattaaa ttctacagcg gacaagccac caggactttt
      961 tgactttgtg acgtcgctcc gtgaggcagg tatcaatcca gttggcttgg tagacccaaa
     1021 ggttccacct aatgaatttc aaatcatcat aggaaggaag ccagggtaag ccgcatctcg
     1081 aatcttggct gttaggcgtt tgtattcaag attagggaat agaattaatt tgataatcgt
     1141 atcgcggaaa aaatcgggac tcctgctatt gttgaaccag aagcacaatt cgcccgggta
     1201 gtccgaaaaa tccttcgggc cggctctatc ttcgttaccg gcgaggtctt gcgggcgggc
     1261 cgaaggattt ctctgggagt ttgctgaaaa acctttcttg cgcggaattc cgagcgcacc
     1321 tagggatccg cacttgtgaa aaaccaagca aaaagcagat tcctcgtcgc cgctggctcc
     1381 tcggaatgac agacggttga cttcttcaac aaactgctag gggacctgcc agtcgagagg
     1441 aagaatgtcc gcgggccttt tcaggcgctg tggaagcgca ggtccagggc gagaagaaag
     1501 cttatgtcaa acaaggcgtg aaagcagaca tgcagaaggt tggggcgggg gatggccgcc
     1561 cgctggccag gccgggacaa gttccggcgg aagcgaccag agaaagtgac ctgaggggcg
     1621 cccgccgaag cgtgttgagg gaaggcggtt gacaagggga atttctccgg gcagagtaag
     1681 gaaagaatga aagtactcat tggcctgctg atcatcggct gcgcgactgc gctcgcccag
     1741 gtctctggaa cacagagcga caatcccgtt cccgcgtatc acagcacgcc tccgggaggg
     1801 ggcgcattga ttgctcccat actgaccccg aaacagctcg cggccggggg atttacccaa
     1861 ccggtccaaa cggaatccta caaagcagcg gcaagagttc ccggtgtgct gtatcagctg
     1921 ccgtgctatt gcttttgcga ccgcgcgcac ggccacacca gcttgcgcag ttgtttcgag
     1981 agtacgcatg gagcgaattg cggcacttgc atggcggaag ctctctatgc ctaccagagg
     2041 tcgaagaagg gctggacggc caggatgatc cgcgacggta tcgtccacgg ggactggaaa
     2101 ctgattgacc tgcagcatcc cgcgccggtt cactaggttg tactccttcc cccgttgaag
     2161 catccaggaa tcctgcacgc caacaatggt cacttcgtca ggggcgagcg tcgtttggtc
     2221 tgacgaactc gcagacgcgt cctattggga cgggtctaaa taggctcgaa gacgggatct
     2281 ttgccgatga cgcggcgcca gatctcggcg aagcgctgct ggttggcggg agtgtcgaag
     2341 gtgcattcga gccgggtgtg gcgaaaggcc gtgtggccga gctggccggc gtgttcgaga
     2401 gcgtagtccc acttcaccgt catcccgggc agaccgtggg tttcccagta gcagtaaacg
     2461 gggaagtccg aggtttccgt acaagtgacc accgtgattt ccacgccgag ggccgggctg
     2521 ccgcagcagc cggtctgcgt ccgccgaatg tcatcctcgg tttcggaagt gatgaccgcc
     2581 gcgggcagag aaaagccggc cacttcgcgg aacaacaggc caaagaaagc ttcggggctg
     2641 ggcgtcgagc agatggcgtc gtagtcgctg gtgtcgggct catccggggc gccttctttc
     2701 aaacgcaaat gcagcatcgc tcgaaaccgt ttcgcctttc cgcaggtgat tgtaaagcaa
     2761 gggagcgaaa agctgtaatc ttgcgcggtt ctggccggat cctgttcaca aacttgatgc
     2821 gcccaagagt tcatcaagac aaactgagat agcccccgcg ctaagccggc tccgaggtaa
     2881 cgccgaaagc accgctcgtt cttctacaga ggcggacgag aggccgcttc caggcggctc
     2941 atgtataatt caggagggga ttccccgggg caattcgcga aggagcggca gtgtccgcat
     3001 gagccgggaa tgggcatgag acaggggtac ggcaaatcgc acgcatcgct gcgggtgtgg
     3061 ttctggcgcg catggcgctt tttctacacg cgggcgcgcg aattgcgcat gatcgtgaag
     3121 gcgctgctct cgaccgggca tcccgtcctg gtgcacatca tccccatccg ccggtgcaac
     3181 ctggcgtgca cctactgcaa cgaattcgac gatttctccg atcccgtgcc gctcgaggaa
     3241 atgaagcggc ggctggacct tctcgcggac atggggacct ccgtcatcac catcagcggc
     3301 ggcgagccgc tgctgcatcc ggagctggac gaaatcatcc ggcacatccg ccggcgcggg
     3361 gtcatcgccg ggatgatcac gaacggcttc ctgctgtctg aggaacgcat cgagcaactg
     3421 aacgccgcgg ggctcgaaca cctgcagatc agcatcgaca acacggtgcc ggacgaggtg
     3481 tccaagaaaa gcctgaagac gctggaccag aagctcgaac tgctggcgaa ctacgcttat
     3541 tttcaggtga atatcaattc ggtgctggga agcggcgtga aggaccctga ggatgcgctg
     3601 cggatcgcgc atcgcgccgt ggacctgggg ctgacgagca cggtgggcat catccacgac
     3661 cagaacgggc agtttaagcc gctggggccg cgggagatcg agatcttcga agagatcatg
     3721 tcgctgggga aacggtcgta ttcgcggttc aacgcattcc agcaccaggt ggcacggggc
     3781 aaagagcacg agtggcgctg ccgctcgggc tcgcggtatc tatacgtgtg cgaggacggg
     3841 ctggtgcact ggtgctcgca gcaacggggc tatccgggga ttccgctggc ggcgtacaca
     3901 ccggagatac ggcggcgcga attcaatacc agcaagccct gcgcgccggg gtgcacgata
     3961 tcctgctcgc agcaggtggc gatcctggac aactggcgcg cgccgcaaac cctgaagccc
     4021 atgccgatga caccgcccgc aacaccgcaa cacgacctgg tgcagctttc ctcgccgcag
     4081 cgctgaacct ccgatccttc ctcaatcgat gaaagactcc agcggaagcg cggtgtgttt
     4141 gccggaataa cacaaccatg cggctggcgc attgacgcag aggcggcctg ccgaaagaat
     4201 agggccctgc ctgggcccgc gattgacatg gaaggactga cgaaggacta cgccgtcggc
     4261 ttcaccgcac tagacccagt ggctcgagac ttacgtacgc tcgcagttgt ttgccgtgct
     4321 ggcatccgtc cgctccgcag gcttattccc taaccaacaa aacgcttgac acgacatttc
     4381 ccatcgtgct aagtgagcgc ctctgaagcg gaatggtccg cttgtcagac cagacgagga
     4441 atccttcctc gccgtgcgcc tgaccttagg ggtgtgctct ggggaactta gccgtactcg
     4501 gcccttccgt gcccgcacgc gtcggggaga cgtcttttcg tatttcattt ctggggagga
     4561 gataaatatg cgatcaatga aaggctgtct gcttttcgcc tcgttccttc tcttgtcggc
     4621 gccgttctcg cgcgcacaaa gcggtgaatc tcccggcaag ggtgactctg catcctcctc
     4681 cggcgcgacg aagcaggagg tcgatcagtt gcgcaaggaa ctggctgcgc agcgccaaac
     4741 cattgagcaa ctcaaggcca tgctccagcg cttggtggag accaaggcgc aaccggcgga
     4801 gggcgctcgc cttgtgaacg ccaacctcga cgtgacggcg caaatgcaaa cgccaaacca
     4861 ggagaaggag caaccgcagg ctgccacctc caagattctg cgcataccag gtaacgagct
     4921 caaattctcg gtcggtggcg gcgaggtcca gatctatggc cacgccgacg tttcgttcga
     4981 ctacgtcgac aatggcctag ccaaccgttt cggcgcggtc ggcaacaatg gctggctggc
     5041 ccaggtgtcg agcaacctgt cttatttcgg cgttcgcggc tcgcgtcggc ttgtcccgca
     5101 tctgaacggc gtattccaga tcgagacgga ggtagctttt tcgtcgaccc ccggaccgac
     5161 cacggacgcg caagtcaagc agggttttgg ctcgcgcgac acctttgtcg ggttgcaggg
     5221 tgactggggc gcggtcaagc tcggcaagga agacgctccg tacaagagat cgactgcgcg
     5281 catggacccg ttcatcaact cgatcggcga tagcagaagc atcatgggca acagcggtgg
     5341 cgacaatcgc tccgagttca atggccgcca gccgcattcg gtatggtacg agtcgcccaa
     5401 gttcaagggc ttctatgcga gcgttctgtt ctctcccgga cagaaccgct cgaaagacaa
     5461 taccatcctc gcgcgcgtgg aaccgaactg tgccgggggg aatgatgcac cctgcaccga
     5521 cggcgccttc ggtaacgttt tgagcgcggt ggtttcttac acctacggat cgctctatgc
     5581 cttcaccgca tacgagcatc acgccaaagc caatcgtaca ggcgatgaac tggcctccat
     5641 cgcggacccg agcatcccgc cggccggatc ggtcggtatc gccgatgaaa gcgcctggaa
     5701 actgggtgct cagtacgagt ttaaactgac ccgtacgacg gcgaatttcg tctatgaaaa
     5761 actgaagcgc tacgctcccg ccgtccctgc cttcaatgag cgctcccggc ctaacgctac
     5821 ctggttggcc atcgtccaaa agatgacgcc gaacgatgac ctcaatttcg gctgggcgca
     5881 cgcgggcaag actcccggcg acccgggcgg gcagatcaat caacccggaa ccgtgatcgg
     5941 gattgcgggg cccatcgaca acaggtccaa cctgatcgac gtaggttaca agcatcactt
     6001 ctcagacaaa aggacctcag catatttcgt gtttgcgacc cagatgaatc atcagggtgc
     6061 ccattacgac ctgggtgcaa atggacacgg cgccgttgtc gaccgcgcgg acgctgccgg
     6121 aaactccttc accgggttga cacacaaggg gctttcagtc ggactgaact acgattttta
     6181 acagtctgat tcggagatcc ttattcctgg tgggcgcacg gatacatcac ggcgcgcccg
     6241 ccaggccgct cggtcggcgt gcttgttcga cttgaaaaag tgtcttaatc tcgctgacgg
     6301 cactcaacgg tgaagaagcc cgggccggaa caaccggccc gggctttctc atcttcctcg
     6361 gtccccttcg ctcgtcaacg tacgggcttc tcctggtagt accgcgcttt ccagtgtccg
     6421 tcccgcatca gccacaagtc gctcaccagc attcgcttca gcttgacctg tgccttcagt
     6481 ggaaactcga tcgtgacttc gtagtcacga acgccgtgtt cggaggggac ggggttgttc
     6541 gatttcctgt ttcaggtgcg atttgcggga gcagccccga gcgagcggag ggaagcgtaa
     6601 cccaggagga taatcgccac gccgagaacg acccagagca gggcggggtg gcgttcggtg
     6661 aagggccgcg ggtcggcgta attggaagtt gtctcttccg gtcccagggc ggcgttccgg
     6721 ggcgcaagag tccgcgtgac ggcagaaggc agcgtcctcc cgagatcgta ttctggagtg
     6781 ccggcgcggg cgtttccgta gagcaagcga tagctgcggc cgggttcggg aatgatcagg
     6841 atcccgacgg aatagcgcgc cagcacaggg cgcacgttct cgagtggcgc atcgttgccg
     6901 ttgaccactt ccacgcgcca gtaccgcgcc cgccaatact cggaaaccgc tacccgcaga
     6961 gattcttcca gtttttctcc gacatggaag cgatagatct ctcccccggc cgcgaaccgc
     7021 cattctttgc tgtcctcgct cgtgaggatg cggaccgcgc ggaagaactc cggctgagaa
     7081 gtttcgaaga cgacttcgtt cacggggatt tggtccgtgc ccaggtcggc gctccagcgg
     7141 ctgagcgtgg ccgggcgggc cgcgtcgggc gtcaggtccg cggagaggcg cgcgcgtatt
     7201 gcctcgcggg cgccggtgga gaaggctacg gaggcggcgg tgacggggaa ctcgtgggac
     7261 ttttcaaaga tgcgcaagcg gagatagcgg gcgttggtgt cggaatagcg gatggtctgg
     7321 ttgccctgca aggtctcttt ccggaagcgg gagatggggg cgcgggcatt gacaattcgc
     7381 caggtgcgag catcgtcgct gacggccact tccacccaga ggatgaagtc ggactcgggc
     7441 gtttcgactc ggacgcggtt gtgaaaggcg gcgtgtaggc cgaggtcgag aatgacttgc
     7501 gagtattgcc cggggacgtg gcttctttcc cgcagggtga ccgagcgcga ctccgtttga
     7561 accgagggtc ggtccgttat gagcacgaag ggaacctccg cgccgcggtc gtctatgacg
     7621 cggaggtccg cgagatgatt ctcggaatga aagaatacgt cctccgggag gaggagttcg
     7681 tagcggctgc cgggctcacc ggccggaagc gtgcgcgagt agcgccagga atgccaggcg
     7741 tccggaaggt cagcagcgag cgcgacggcc acaacgaccc cagccagcag gcagagaata
     7801 aggctgcggc gcatcatggg cgggtctgcc tgcagacgag tttgcgctga tacaagaacg
     7861 acacgaccac aagaagcagg ccaaggatca ggaaggagag aatgcggtag aagcgctcga
     7921 gataggagga gtcgtagatg aagaccttgc cgacgaccag gccgaacagg gccagggctt
     7981 gccagcgcag tagagcggac tggcgcttga cgccgagaag gatcagggcg gtggcgtagc
     8041 aggtccagag gacggagaga gcgagatgct ggctgaggtc gcggtcgagc gcggtggcgc
     8101 ctgcgcggcc ataataatcc caaagctcga gggaaagagc gacgagtgtg aacccattga
     8161 tggccacggc gagaacgccg taggcctgcc gctcgttccg gcccaggctg ctccattgct
     8221 tgcgggctgc gaccacggag acggcaaagc acgcgatgac cacggcaaag gcggcgaagc
     8281 gggcattgag aaggaattgc ggcgcgggaa tcggcagcaa tagcaggcgg agcgcggcga
     8341 ccgagaggag gaaatagccg gcgtggcgca gaagcggcag gagcgcgcgc aagccgctcc
     8401 agatgaggat ggcgccttcg atggcccagg caagcgtgat ccacttgcct tcgaggcgga
     8461 tcggaatcgc cagggtcgca aaagtgagag cgaggccggc gaagaggatt cgagtccagg
     8521 gagattctcc cgctttgggt gcgggggcca tccgcgccac gaaaaggtgg gtggcggaga
     8581 gcagaagcac agcgacggtc agcgcccagc ggtgctcgtc ccagagcatc acccgcaggg
     8641 ccagcagata gatgcccgcg ttgagaagaa cgaggatcgg atccagccag tcgaggctgc
     8701 cgtggcgggc cgcgcggacc accggtactg ccgcgtagag gacgaagaaa agcgtggcga
     8761 agaagacggt ccgcgcaagc acctcggagc gatagaaact cccataccaa ccccagaaat
     8821 aggcctgcgt tagcacgaag gccaccggcg ccaggtagcg ccaatcgcgc ctggcggcga
     8881 tgaaaagcat tccggcgccg aggatcaaca ggtacgtgaa aaggaccacc tgctggtctc
     8941 tgcccgtgct gacaatgatg ggggtgagga ggccgccaag cagggagagg agagcgatgc
     9001 gctgggagtc gcggccgatg gccacagcag ccatcacggc ggtgacaacg atcatcccgg
     9061 cgaaggcggt ggtctgggag aagagtgcgt aatagtgcca gccggcccag atggagacat
     9121 agagaacggc ttcaccgagc ccggcaatgc cttcggagaa gtaggtgtag ccgcgagcca
     9181 agagccattg gctccaggga agcatggcgc ttccgaggag aatgccgatg cccacgcggc
     9241 ctctcgggcc gatccagttg ttgtcgaagg
//
LOCUS       DASYVO010000374        10112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_55752, whole genome shotgun sequence.
ACCESSION   DASYVO010000374 DASYVO010000000
VERSION     DASYVO010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 10112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10112
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55752"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VGR03_12680"
     CDS             <1..182
                     /locus_tag="VGR03_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995177.1"
                     /translation="LFAAGMPVIHMRGAHYGEIAKSTGGFFFVWTLWALGALGGFTFI
                     LSACGLWSLRPGQPR"
     gene            561..1358
                     /locus_tag="VGR03_12685"
     CDS             561..1358
                     /locus_tag="VGR03_12685"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEV1995178.1"
                     /translation="MNRKLIIAVFFISVFLAAPSALRGQSRDELKLFNLMNQEREKAG
                     LLKFQWSADLAKSARAHAQLLTEHRELSHRFTGEPVLSDRIGATGLRYDAAAENVASA
                     AEVEDAHAALMKSPGHRANILNPKYNAAGLAVVPHGARVYVAQNFAHVLPVYSETQFR
                     RDVVAAVNKLRQSKGIGAILARADAALRDAACAENPNLQRLIRTLPGVTHLVAFTSSV
                     PDKLPESLDKAVSDATMGRMNIGVCFRPGPEHGFASFSILAAFYSAN"
     gene            1526..3661
                     /locus_tag="VGR03_12690"
     CDS             1526..3661
                     /locus_tag="VGR03_12690"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEV1995179.1"
                     /translation="MFRKAALAIALLGMIFAASRGANAAPQQAGKSPARTAGASSRKA
                     AAHPRAADSKSVKRPKSFDLDAMDKSVAPCEDFYEYACGNWRKNNPIPRDQARWGRFN
                     ELAEYNRQLLHEILEKASVRNRKRGPITQKIGDLYQSCMDEKTVNAKGAVPLKPALDR
                     IAAISNRDQLIDTIAYLHSQGVGALFGFGAQPDLHNASMEIAGVFQGGLGLPDREYYL
                     KQDPKSQETRQKYQEHLVNMFVLLGDARDAAEKEAQAVMSIETKLADAAFERVKMRDP
                     KNRDHKMKVSELGALAPSFQFQRFFEASHAPAFAEVNVVPPDFFQKVNATIADVSLDD
                     WKSYLRSHAARTAAPLLSEPFVQENFGFFGRYLNGQKELQPRWKRCVQLTDSNLGEAL
                     GQPYVAETFGKEGKERMLKMVEALEKALAQDIQNLPWMTEVTKKQALVKLRAITNKIG
                     YPDHWRDYSSVKIVRGDLVGNILQTRGFERRRNLNKIGKPLDKKEWGMTPPTVNAYYN
                     AAENDINFPAGILQPPFFDKNADDAVNFGGIGVVIGHELTHGFDDQGSKFNAEGNLTN
                     WWTDADRAEFDKRTKCVSDEYGSFVAVDDLHLNGPLTLGENTADNGGSIIAMMALHSA
                     ILSGAKSVGAGQVKDGFTPEQRFFLSFAQIWCQNSTPENSRLLTKTDTHSPGRYRVIG
                     TLQNNADFAKAFGCKAGQKMVSENACRVW"
     gene            3792..4673
                     /locus_tag="VGR03_12695"
     CDS             3792..4673
                     /locus_tag="VGR03_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995180.1"
                     /translation="MHRGRGQRSGKGGERVGLSAVLVLSVAWLALPAAAQQPGPIKPP
                     PAPAAAPSKGSSAISNELPSIPVEQIIQRFAERESELRKERENFAYEQSFIFQTFDLN
                     GQPDGEYRMDSDVLFTPLGKRYEKVTYAPPPTLVRLGLTQQDLSDLQNVQPFLLSTEE
                     LPKYDVRYAGRQRVDELTTYVFDVAPKTIEKNQRYFQGRIWVDDRDLEIVKTEGKAVP
                     DFKGKNENIFPRFVTYRENIEKNFWFPTYTSSDDVLHFRSGDVRIRIKVRYSNYKRFR
                     SSGRIVGPAEPVPPDKP"
     gene            complement(4748..5194)
                     /locus_tag="VGR03_12700"
     CDS             complement(4748..5194)
                     /locus_tag="VGR03_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995181.1"
                     /translation="MTRESPNSHSVNDLSRCQHRTLRGRRCRFRVAGPDSPLCFRHFA
                     LCAAKGIQFGRQAADLSPELVGQLTEFKSALDINRVLSNLLLLISRDRIAPRRASVVA
                     YTCNLLLQSLAAIDFELHPPVGKEEEIVQIIVDTPRPIRDRPNQRS"
     gene            5745..6788
                     /locus_tag="VGR03_12705"
     CDS             5745..6788
                     /locus_tag="VGR03_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995182.1"
                     /translation="MRRRFCGVLLWLVTGAAALGQPALAGEKSAPKLRQLTAREGRGI
                     AKVADELESAMGRKPDCSHLVHQVYAQAGFPYPYADSLDLYKGWERFLRVPVPQAGDL
                     VVWRGHVGIVMNSREHSFYSSVRSGLRTEFYDSDYWRARGRPRFYRYLAEGVQAARQP
                     RQDSRPPMHPVQAKSATPGTARSETSTAGEMRREMAPETAIHAARKEPMAEEVAEALA
                     ERNAAGGEILRAGNPARLVIVYRELRVTDVEIKGKKGTAHVEIDTLASLEGERMESQP
                     GTREFATELRRTKKGWVVTGEKERAYVPGDVAMRILAARLAVLTQDAGKSAQSVREQA
                     QIIRLLNLLIQEN"
     gene            6985..7443
                     /locus_tag="VGR03_12710"
     CDS             6985..7443
                     /locus_tag="VGR03_12710"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEV1995183.1"
                     /translation="MKKMAIGLLLLLTFSAPAFSQECTSKLQGFVNKTGYKFKVAKPC
                     QVWVATDALTIPRGEGVTGVLLIGQSGDVVVIGTVVQPKAKLDLSADVLLKLMQLNNE
                     LEFVKVGIDNDGDLFVRAELRVGSMTAEDFSASVKKVVEASTQVYATLKK"
     gene            8305..>10112
                     /locus_tag="VGR03_12715"
     rRNA            8305..>10112
                     /locus_tag="VGR03_12715"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         2328 a         2802 c         3224 g         1758 t
ORIGIN      
        1 ggttgttcgc ggcaggcatg cccgtcatcc acatgagggg ggcacattac ggcgagatcg
       61 ccaagtccac cggcggcttc ttcttcgtct ggacactctg ggcgctcggc gcgctcgggg
      121 gcttcacctt catcctctcg gcgtgcggac tgtggagtct acgaccgggc cagccccggt
      181 agtccaacaa cccgaacatg gaaaggcccg tcgcacggcg taggtgatcg gcgcggatcg
      241 ggtggcaaca ccatcattct cgtcacccac gaacatgaca tcgcccagca tgcccaccgc
      301 agcatcttca tcaaagacgg cagcatcttc tccgaccagc ccaccaagaa gtagctggga
      361 taaaagcaag gcattcgccg catcgcggcg gcgtataggg tttcgcggcc ggcgccaacc
      421 ctttctgctg ctggccctcg ccctggggcc gggtcaacgg aacgccaaat gttttcttca
      481 ccgggttgcc gtgcaaaccc cgcaattgaa aatggcttga gggttgactc ggaagagggc
      541 cgattccaaa attagcgtaa gtgaatcgca aactcatcat cgcagttttt ttcatttccg
      601 ttttcctcgc ggccccatcc gccctacgcg gacagtcccg ggacgagctc aagctcttca
      661 acctgatgaa ccaggagcgc gaaaaagccg ggctattgaa attccaatgg agtgctgatc
      721 tggcgaagtc ggcccgcgct cacgcgcaac tcctgaccga acaccgggaa ctctcacatc
      781 gattcaccgg cgagcccgtc ttgagcgacc gcattggcgc caccggactg cgttacgatg
      841 ccgctgcgga aaacgtggcc tccgcggccg aagtggagga cgcgcatgcc gctttgatga
      901 aatcgccggg ccaccgggca aatatcctga atccaaagta caacgccgcg ggccttgccg
      961 tcgttccgca tggcgcgaga gtttacgtcg cccagaactt tgcccacgta ctgcctgtct
     1021 attcggaaac gcaatttcgc cgcgacgtcg tggcggcggt caacaagcta aggcagagca
     1081 aggggattgg ggcgattctg gcccgggccg acgcggcttt gcgcgatgct gcctgtgccg
     1141 agaatcccaa tctgcagagg ctgatccgga ccctcccggg tgtaacgcat ctcgtggcgt
     1201 tcacttcgtc cgtgccggac aagttgccgg agtctctgga caaggctgtc tcggacgcca
     1261 caatgggccg gatgaacata ggagtttgtt tccgcccggg gccggaacac ggttttgcca
     1321 gcttttccat cttggcggct ttttattcgg cgaattgagt agcgatcggt attttgcggt
     1381 caacctcagg gcctgtgcct ggctccgcag cgtgacttga aatccttcat acctccggct
     1441 cctcccgcgg agaacggatt gacgaagacg acgattgggc ataaattcca acattccttc
     1501 aagcattcca cgaatcgagg tgattatgtt ccgcaaagca gcactggcca tcgcacttct
     1561 ggggatgatc ttcgcggcat cgcgcggcgc caacgccgcc cctcaacaag ccggaaaatc
     1621 gccggcacgg actgcgggcg cgagctcccg caaggcggct gcgcatcctc gcgcggcgga
     1681 ctcgaaatcc gtcaagcggc ccaagtcctt cgacttggac gcgatggaca agagcgtggc
     1741 tccgtgcgaa gacttctacg aatacgcttg cggcaactgg cgcaagaaca atccgattcc
     1801 ccgggaccag gcccgctggg gacgcttcaa cgaactggcc gaatacaacc gccagcttct
     1861 gcacgagatc ctcgagaaag cctccgtcag aaaccggaag cgcgggccta tcacgcagaa
     1921 gatcggcgac ctctatcagt cctgcatgga cgaaaagacg gtgaacgcca aaggcgcggt
     1981 gcccttgaag ccggcactcg accgcatcgc cgcgatttcg aatcgcgatc agctcattga
     2041 cacgattgcc tacctgcact cccagggtgt gggagcgctc ttcggcttcg gtgcgcagcc
     2101 cgacctgcac aacgcgagca tggagattgc cggcgtcttc cagggcgggc tggggcttcc
     2161 cgaccgcgaa tattatctga agcaggaccc gaaatcacag gagacccggc agaaatatca
     2221 ggaacacctg gtcaatatgt tcgttctcct gggcgatgcc cgggatgcgg cggaaaaaga
     2281 agcgcaggcg gtgatgagca tcgaaacaaa gctcgcggat gccgcattcg aacgcgtcaa
     2341 gatgcgcgac ccgaagaacc gcgatcacaa gatgaaggtc tccgagctcg gggcgctcgc
     2401 gccgagtttc cagtttcaac gcttcttcga ggccagccac gcgcccgcct tcgccgaagt
     2461 gaatgtcgtt cctccggact ttttccagaa ggtcaacgcg accatcgcgg acgtttcgct
     2521 ggacgactgg aaatcctatc tgcgttccca cgcggcacgg accgccgctc cgctgctctc
     2581 cgagcctttc gtccaggaaa acttcggctt ttttggacgc tacctcaacg gacaaaaaga
     2641 gctgcagccg cgttggaagc gctgcgtgca gttgacggat tcgaatctcg gcgaggcgct
     2701 cggccagccc tatgtcgccg agaccttcgg caaagaaggc aaagagcgca tgttgaaaat
     2761 ggtggaggcg ctggagaagg ccctcgcgca ggacatccag aacctgccct ggatgaccga
     2821 agtgaccaag aagcaagcgc tcgtgaagct gcgggccatc accaacaaaa ttggatatcc
     2881 cgaccactgg cgcgattaca gctcggtgaa aatcgttcgc ggagacctgg ttggaaacat
     2941 cctgcaaacg cgcggcttcg agcggcggcg caacctgaat aaaatcggca agccgctgga
     3001 taaaaaggaa tggggaatga ccccgccgac ggtgaacgcc tattacaacg ccgccgaaaa
     3061 cgacatcaac tttcccgccg gcatcctgca gccgcctttc tttgataaaa atgcggacga
     3121 cgccgtgaat tttggcggca tcggcgtggt aatcggccac gagctgaccc acgggttcga
     3181 cgaccaggga agcaaattca acgcggaggg aaatctcacg aactggtgga cggatgcgga
     3241 ccgcgcggag ttcgacaagc gcacgaaatg cgtctccgac gagtacggct cgttcgtggc
     3301 ggtggacgac ttgcatctga acggcccgct gacactcggc gagaacacgg cggacaacgg
     3361 cggctccatc atcgccatga tggcgctgca cagcgcgata ctctccgggg caaagagcgt
     3421 gggcgccgga caggtgaagg acggcttcac gccggagcag cgcttttttc tgagcttcgc
     3481 ccagatctgg tgccagaaca gcacgcccga aaactcccgg ctgctgacga agacggacac
     3541 gcactcgccc ggacggtacc gcgtcatcgg cacgctccag aacaatgccg atttcgcaaa
     3601 agccttcggc tgcaaagcgg gccagaaaat ggtgagcgag aacgcctgcc gcgtctggta
     3661 attcgccgcg gaagccatct gccggggcct gttcgctcgg ggtagaactc ccccgccgga
     3721 agcaacccgg cctcttgaaa cgtgttctca tcctctgaga cggaaccaga gaaagaagga
     3781 gacgcacggc agtgcatcgc ggcagagggc aacgctccgg aaagggcggt gagcgcgtcg
     3841 gcctctcggc cgtgctggtc ctcagcgtcg cgtggctggc tttgccggcc gcggcacagc
     3901 agccgggccc gatcaagccg ccgcctgccc ccgccgccgc tccttccaaa ggctcctccg
     3961 caatttccaa cgagcttccc tccatccccg tggaacagat catccagcgc ttcgcggaac
     4021 gcgagagcga gctcagaaag gagcgggaga acttcgctta cgagcaatcg ttcatcttcc
     4081 agacgtttga tctcaacggg cagccggacg gcgagtaccg catggacagc gacgttctct
     4141 tcacgccgct cggcaagcgc tacgagaagg tcacttacgc gcctccgccc acgctcgtga
     4201 gactcggtct cacgcagcag gacctgagtg acctgcagaa cgtccagccc ttcctgctct
     4261 ccaccgaaga gctgccgaag tacgacgtcc gctacgccgg ccggcagcgc gtggacgaac
     4321 tgaccaccta cgtcttcgac gtggccccga aaacgatcga gaagaatcag cgctactttc
     4381 agggccgcat ctgggtggac gaccgcgacc tggagatcgt caaaaccgaa ggaaaagcgg
     4441 tgccggactt caagggcaaa aacgaaaaca tcttcccgcg ctttgtgacc taccgcgaaa
     4501 acatcgaaaa gaatttctgg tttcccactt acacaagctc cgacgatgtg ctgcactttc
     4561 gctccggcga tgtgcgcatc cgcatcaagg tgcgctactc gaactacaag aggttccgct
     4621 cgtccgggcg gatcgtcggt ccggccgagc ccgtgccgcc cgacaaaccg taggagtcca
     4681 cacgcttcaa cagaaattgt tttcgcaggg gcgttcactt ggcctccgcg gggatgggtg
     4741 gcgggagtca tgagcgttgg ttgggccggt cgcgtatcgg gcgcggggta tccacgataa
     4801 tctggacgat ctcttcctcc ttgccgacgg gcggatggag ctcgaaatcg atggccgcga
     4861 ggctctgaag gagcaggttg caggtgtagg cgacgaccga ggcgcggcgc ggagcgatgc
     4921 ggtcgcggga gatgaggagg aggaggttgg agaggacacg gttgatgtcc agggcggatt
     4981 tgaactcggt gagctggccg acgagttcgg gggagaggtc ggcggcctgg cggccgaact
     5041 ggataccctt cgctgcgcac agggcaaagt ggcggaagca gagcggggag tccgggccgg
     5101 cgacgcggaa gcggcagcgg cgtccgcgga gtgtgcgatg ctgacagcgg gatagatcgt
     5161 tgacggaatg ggagttaggc gattcgcggg tcatgatttt tcctcagaga gggattgaca
     5221 aagggggcca ccccctattg tttttgcaag agtgcgtaaa tcgaagaatt caaaaggttt
     5281 gtttggattt ctgcgcagct caagagtgaa aagagtgcgg aaggagaagg aaggaaagga
     5341 gttagacaga aaaggaatgc gaagcaacga agtaatgaag taacgaggta atgagagagc
     5401 cccgagggtg ggcgagagcg tcacaaccca tgatagcacg gaacgttgtc tctgtcaata
     5461 gagtactatg gagtactagt ttcgaatggc gcgaggtagt gtcgaatcag cggccggcaa
     5521 atgcaagaaa tgaaagtgcg aatgcggggg gcggagagac gggtcgagtg caggttcact
     5581 ccacaagaga gaaggccaag agtcggcagc catcgagaac ccccacgcgc aacgccggcg
     5641 catggggcac ccggcactgg gcgcgagcct gtacggaagg acggtaatgg ctttgcgaca
     5701 aggccagggc ggggtggccg tatctttgcc aaactcggga aaggatgcga cgacgattct
     5761 gcggcgtact gctgtggctc gtgaccggtg cggcggcgct ggggcaaccc gcgctggcgg
     5821 gggagaagtc tgcgcccaag ctgaggcagc tgactgcccg ggagggacgc ggaattgcga
     5881 aggtagccga cgaactcgag agcgcaatgg gccgcaagcc ggattgctcg cacttggtgc
     5941 accaagtgta cgcgcaggcg gggtttccgt atccctacgc ggactccctc gatctttaca
     6001 agggatggga gagatttctg cgcgtgccag tgccgcaagc cggagacctg gtggtctggc
     6061 gcgggcacgt gggcatcgtg atgaattcca gggaacattc cttctatagc tcggtgcggt
     6121 cgggactccg gacggaattt tacgattcgg actactggcg tgcgcggggg cggccgcggt
     6181 tctatcggta cttggcggag ggcgtccagg cggcgcggca gccgagacag gattcgcggc
     6241 ccccgatgca tccggtgcaa gcgaagtccg cgacgccggg cacagcgcgc agcgaaactt
     6301 ccaccgcggg cgagatgcgc agggaaatgg cgccggagac ggccatccat gcagcgcgga
     6361 aggagcctat ggcggaagaa gtcgccgagg cgttggcaga gaggaacgcc gccgggggag
     6421 agattcttcg ggcgggaaat ccggcgcggc tggtgattgt ctaccgggaa ttgcgcgtga
     6481 ctgatgtgga gattaagggc aagaagggaa cggcgcacgt cgagatcgat acgcttgcct
     6541 cgctcgaggg ggagcggatg gaatcgcagc cggggacgcg cgagttcgcg acggagctgc
     6601 gccgcacaaa aaaagggtgg gtggtgacgg gggagaaaga gcgcgcgtac gtgccggggg
     6661 atgtggcgat gcgaatcctg gcggcgcggc tcgctgtgct gacgcaggac gccggcaaga
     6721 gcgcacagag cgtacgcgag caggcgcaga tcattcgctt gctgaatctc ctgattcagg
     6781 agaattagcg cctgagcagc ggggcttcct cgcaacgagg gtgctcggca agtcatggat
     6841 cgcgccactt ccgaggctgc gattccacaa gaacttggcc aagagccagc cacccactta
     6901 ggaatcctag gcgcaacgcc ggcgcatggg gcacccggcg cggagcgatg tagaatggtg
     6961 tcgcccccaa gactggagaa aatcatgaag aaaatggcca tcggccttct gttgttgctg
     7021 acgttcagcg ccccggcttt ttcgcaggaa tgcacttcca agctgcaggg attcgtgaac
     7081 aagaccggct ataagttcaa ggtagccaaa ccctgccagg tgtgggtggc taccgacgcc
     7141 ctgaccattc cccgcggcga gggcgtcacg ggagtgctgc tgattggcca gtcaggcgac
     7201 gtggtcgtta tcggcaccgt ggtgcagccg aaagccaagc tcgatctttc cgccgacgta
     7261 ttgctgaagc tgatgcaact gaacaacgaa ctggagttcg tgaaagttgg tatcgacaac
     7321 gatggagacc tgtttgtcag ggccgaactg cgcgtgggaa gcatgaccgc cgaagacttc
     7381 agcgcctcgg tcaagaaagt cgtcgaagcc agcacccagg tttacgccac gctgaaaaag
     7441 tagagagtgt cggcgcaaga gagcccaggt caaaggcccc acccttcgct gcgcaaagga
     7501 tgggccaccc gcccacgcgc aacaccggcg catggggtac ccggcaggtc ggggcccgat
     7561 tttcacagaa tgcacagggg gaggaaaaaa ggagaaggca gggggttgac gaggggcgaa
     7621 aattgggtat actgggtgat ggtttgggga gacccgggcc aaccaggggc gtcacggtcg
     7681 gtctgtaaca aatttggacc caagtgaggc aggttacgag tacaggatag aaatcagtcg
     7741 agctacgcgc aaagccgcca ggaagtggcg gtttggcccg gcgctcgacg ccgggttttt
     7801 tattttccgg cgacagaaga tcgtcgccgg aaacactgca gagctggaag gcaagatcgc
     7861 gcaatgcggt cggataagtc ccaccagaca ggcctcggca accgaggcgt tccgatggaa
     7921 tcggaatcaa cgctctttga caactgaata ggctggcaag ttcacggcga cagccctgga
     7981 ctcttccttc gggaggagaa cggggaatta tgccgtggac gattacaagt tctgctcgag
     8041 tagagcaaaa ctgtggaaca aggcgaattt actcaagaga aggtaactgg cgacgagcga
     8101 ccggtgattc gcaagagtca ttcgggaaat cgttgcaaga tgcctgaagt ctgaggagtg
     8161 cggaatcgaa agccggcttg gggagcaatc tcaaagccgg aagaatggaa ccgcacgaat
     8221 cggcgcgttg aagggaagtc aagagtgccg ggctaaagcc cggcgctaca tggcaaaccc
     8281 ggcgacgacc tgggtaagtt ttatggtcaa gctactaaga gcgtacggtg gatgccttgg
     8341 cgccagaagg cgatgaagga cgtggcaagc tgcgataagc cccggggagc cgcaagcagg
     8401 ctttgatccg gggatctccg aatggggaaa ccctgagccg gtaataccgg cttcacagcc
     8461 cgctgaatac ataggcgggt atgagctaac ccagggaagt gaaccatctc agtacctgga
     8521 ggaaaataaa tcaaagagat tccgaaagta gcggcgagcg aaatcggaac agcccaaacc
     8581 atcgtgcttg cacggtgggg ttgtaggacc gcgtgagtag agttaccaat ccgacggtta
     8641 ggtgagcccg ctggaaagcg gggccaaaga aggtgatagc cctgtaaccg aaaactgatc
     8701 ggactcgaag cggcatcctg agtaccacgg gacacgtgaa atcccgtggg aaactgggga
     8761 gaccactctc caaggctaaa tactctctgg cgaccgatag tgaaccagta ccgtgaggga
     8821 aaggcgaaaa gcacccctgc gaggggagtg aaatagaacc tgaaaccgta tgcttacaag
     8881 cagtcggagg gcaatgtccg ggcttcggcc tggataagcc tgacggcgtg cctcttgctt
     8941 aatgagccgg ctagttattg tcacgggcaa ggttaagcgc aagcgagccg tagcgaaagc
     9001 gagtccgaat agggcgaata gtccgtggct atagacgcga agcgggatga tctacccttg
     9061 gccaggttga aggtcgctta accgcgactg gaggaccgaa ccggtgtgag ttgaaaatcg
     9121 ctcggatgag ctgagggtag gggtgaaagg ctaatcaaat tccgttatag ctcgttctcc
     9181 ccgaaatgtc tttagggaca gccttgtgtg atttgccgcg gtggtagagc actgaatgga
     9241 ctaggcacca actcaggtta ccaaatccaa tcaaacttcg aatgccgcgg acaacagcac
     9301 gggagtgaga ctacgaggga taagcttcgt ggtcaaaagg gaaacagccc agaccgccag
     9361 ctaaggtccc aaactgtatg ctaagtggga aaggacgtat attcgctcgg acagccagga
     9421 ggttggctta gaagcagcca tcctttaaag aaagcgtaac agctcactgg tcaagcggat
     9481 atgcaccgac aatccaacgg ggctcaagca tacgaccgaa gctgcggaat tgaccattcc
     9541 ttcgggaatg gtcatttggt aggggagcat tgtgtggagt cataaggcag accgagagga
     9601 ctgctgaacg ccacgcaaga gaccctgccg gcataagtag cgataaacac gatgagaact
     9661 cgtgtcgccg aaagtctaag gtttcctgag gaaggttaat ccgctcaggg ttaggcgggt
     9721 cctaaggtga ggccgaaagg cgtagctgat ggatagcagg tcaatattcc tgccccgcct
     9781 gggaacgtta tcacgatggg gtgacgcaga aggataggag attcgggtga tggatgtccc
     9841 gatttaccag tgcaagccgg atcccgccgg aaaatccacg gggtcattca acggtgagac
     9901 tggcgacgag gccttacggc cgcaaagtct ctgatttcac gctgccagga aaaacctcta
     9961 aggagttccc caggcgcccg taccgcaaac cgacacaggt agacgaggtg aatatccaca
    10021 ggcgctcgag agaacgctcg ctcaggaact aggcacatta accccgtaac ttcgggagaa
    10081 ggggtaccgc tgtagggttt atagcccgaa gc
//
LOCUS       DASYVO010000375         3222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_217558, whole genome shotgun sequence.
ACCESSION   DASYVO010000375 DASYVO010000000
VERSION     DASYVO010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217558"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VGR03_12720"
     CDS             complement(<1..284)
                     /locus_tag="VGR03_12720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1995184.1"
                     /translation="MASDSLIQYSIVVPFFNEQENIPPLYMKLTEVMDGIGEPYELVF
                     VDDGSKDDSFKVLSEIYEHDRRVNLVRLRRNFGQTSALKAGFDFARGEVI"
     gene            complement(306..1190)
                     /locus_tag="VGR03_12725"
     CDS             complement(306..1190)
                     /locus_tag="VGR03_12725"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEV1995185.1"
                     /translation="MKNLIVNADDLGWTDGVNRGILEAFHHGIVTSTSLLANGPAFAG
                     GVEAARSAPGLGVGVHLNLSDGPPMADRETVTSLLNDHGEFAGGPESLLLRRARGGLP
                     LAEVESEWDAQIQKVRDAGIAPTHLDGHKHVHMLPGLFEIALRLAKRHNVGAIRVSLE
                     ASSLRAALSSGSQRNAGVVIKQGVQARGLKLLARDAREQAERAGISTADYFCGIAQTG
                     ELTREGVEQFVKSLPDGTTELMCHPGYADAALQKTPTRLQDSRQTELQILTDTGIRNL
                     VASLGIRLIDYGFVAQEA"
     gene            1314..1658
                     /locus_tag="VGR03_12730"
     CDS             1314..1658
                     /locus_tag="VGR03_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995186.1"
                     /translation="MPVKLRESKPIGSRHHENRRGRRMNSRVPVRLEWDDESGKRISA
                     EAHTRVINPYGCMVVLGQSLNLEHRLALTNLISATSNAAIIVWKGNQRPDGWEYGIEL
                     LAPEMDFWGLDL"
     gene            1655..2053
                     /locus_tag="VGR03_12735"
     CDS             1655..2053
                     /locus_tag="VGR03_12735"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEV1995187.1"
                     /translation="MKPTSNVKPSPYAKPGSISAVRPAMTRQPTAEERRRAQRVLLRM
                     RVLVHIANKPEPMVGHTHTVSATGGMIILPEGFPQGTKFILENPKTQERVEVNVVRSP
                     QMNQEGSLVPVEFLSPAPKFWNVFFPPEIN"
     gene            complement(2879..>3222)
                     /locus_tag="VGR03_12740"
     CDS             complement(2879..>3222)
                     /locus_tag="VGR03_12740"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV1995188.1"
                     /translation="KITVTSTKVASGTNIAFAIAVTASVGANGQVQLFDGSTALGSAV
                     TVSNGSATINNAGLAPGTHSISAHYLGDTYTQASQSGALNVTSTGTTTFVVSATPAAS
                     NGTPTVSITIN"
BASE COUNT          680 a          961 c          922 g          659 t
ORIGIN      
        1 atgatgactt cgccccgcgc aaaatcaaat cctgctttca acgctgacgt ctgcccgaaa
       61 ttccgccgca gcctcaccag gtttacacgc cggtcgtgct cgtaaatttc agagagcacc
      121 ttgaacgagt catccttgct gccgtcatcc acaaacacca attcgtaagg ctcgccgatc
      181 ccgtccatca cttcggtaag cttcatgtac agaggtggga tgttttcctg ttcgttgaag
      241 aacggaacga cgatggaata ctggatgagc gaatcgcttg ccattcttgt ccgcaaaggc
      301 tttctttaag cttcctgggc tacgaagcca tagtctatca ggcgaatccc cagcgatgcg
      361 acaagatttc taatcccagt gtccgtcagg atctgcagct cggtctgccg cgaatcctgc
      421 agccgggtgg gtgttttctg cagcgccgca tccgcgtagc ccgggtggca catcagctcg
      481 gtcgttccgt ccggcaggct cttcacgaat tgttccacgc cttcgcgtgt cagctcgccg
      541 gtttgcgcaa tcccgcaaaa ataatcggcg gtggatatac ccgcgcgctc ggcttgttcg
      601 cgcgcatcgc gcgccagcag tttcaatccg cgcgcctgca cgccttgttt tatgaccacg
      661 cccgcgttcc gctgcgagcc cgaagaaaga gccgcgcgca ggctcgacgc ttccagcgaa
      721 acgcgaatcg cgccgacatt atgccgcttc gcgagccgca gtgcaatctc aaacagcccc
      781 ggcaacatgt gcacatgttt atgcccgtcg agatgcgtcg gcgcaattcc cgcgtcgcgg
      841 actttctgaa tctgcgcgtc ccactcgctt tccacttctg ccagcggtag cccaccccgc
      901 gctcgtcgca acagcaaact ctcggggccg cccgcgaatt cgccgtggtc attcagaagg
      961 ctagtaacgg tttcccggtc ggccatcggc gggccgtcgc tcaagttcaa atggacgccc
     1021 acgcccaatc cgggcgctga ccgcgcggcc tccacaccgc cggcaaacgc ggggccgttg
     1081 gccagcagcg aagtgctggt cacaatgcca tgatggaagg cctcgagaat tccgcggttt
     1141 acgccgtccg tccatcccag gtcatccgca ttaacgatca gattcttcac agcctgtccc
     1201 gctccctcgt ttgcacctgt cctttgggac agctactctt ttgcgcgccc acccaaatac
     1261 gtaccattga ccgttttcgc cctcaactgt agcatggcgt ggtagaggcg aaaatgcccg
     1321 taaagctgcg tgaatccaag cccattggtt cgcggcacca cgagaatcgc cgtggccgcc
     1381 gtatgaactc ccgtgtgccc gtgcgtctcg aatgggacga cgagtccggc aagcgcatca
     1441 gtgccgaagc ccacacgcgc gtcatcaacc cctacgggtg catggtcgtt ctgggacagt
     1501 cactcaacct ggagcaccgg ctggccctca ccaatctgat ctccgctacc agcaatgcgg
     1561 cgatcatcgt gtggaagggc aatcagcgtc ccgacggctg ggaatacggc atcgagctcc
     1621 tggcgccgga aatggatttc tgggggctgg acctgtgaag cccacttcaa acgtgaaacc
     1681 ttcgccgtac gcgaaacccg gttcgataag cgcggtgcgt cccgcgatga cgcggcagcc
     1741 taccgctgaa gagcgccgcc gcgcccagcg tgttctcctg aggatgcgcg tgctggtcca
     1801 tattgccaat aagcctgagc cgatggtagg tcatacgcat acggtcagcg ccaccggtgg
     1861 catgattatt cttcccgagg gctttcccca gggcaccaag ttcattctcg aaaatccaaa
     1921 aacacaggaa cgcgtcgagg tgaacgtggt ccgttcgccg cagatgaatc aggaaggctc
     1981 actggttccc gtcgagttcc tgtcccccgc gccgaagttc tggaatgtat tctttccccc
     2041 agaaatcaac tagagattct ttccggcagg tccggaatgg tcgatcgtcg cgatgctcct
     2101 ccccgagatg ctcggggcag gcgggacagc gattcccggc aagggggact tcctggtccg
     2161 cgccgggatg caaagttcgc gcctcatcgg attcaagaga ggtcccttcc gacagggtcg
     2221 gaaccttcgg tcggctcccg atggtcccga tgaatcgggg ctcgcaaaca gcgctcgtcg
     2281 cctgggacag aaatccttcc tgggatttct agttaaccat aacaccccca cccccctgtt
     2341 ttttgtaagt gtggcaacta aagggtttag gttttctgta agttctttgt ttaccacact
     2401 tacgagagag ttcataagtg tggaaactaa aggacttacg ggatacagaa aatggaaaag
     2461 tgcaaatgga aaatcggtag gaatcggggg gagaacggtg gcgacggtgg ggaaagagaa
     2521 gggcgagcga gaatctgatt cggtatacac gagaaagaat agcatgggac gggcgtggct
     2581 gtcaaccaga aagatacgtc attgtccagt agcgttcgaa tgggatgtgg aaaagagttg
     2641 ggcacaaggt tctttttgcg aacgctcagg agaaagctga gccgttacaa gaagaaagcg
     2701 cacagccagg aatgtgccac tcaggaaaag gcgcccgcct aagaaggcag ccgctacaag
     2761 gaatccgggg caggtacggg actagcgtgg gagggatata tgttgcgagg aagggcggct
     2821 aaaaaaagag cggccccgga gaatccaggg ccgctccttg aggccagcta gcgctgttct
     2881 agttgatggt gatgctgact gtcggcgtgc cgttggacgc cgcgggtgtc gccgagacca
     2941 caaacgtggt cgtccccgtt gaagtgacat tcagcgcgcc gctctgagac gcctgcgtat
     3001 aggtgtcgcc cagatagtgc gcgctgatcg agtgcgttcc cggtgccaag cctgcgttat
     3061 tgatggtcgc tgacccgttg gacaccgtaa ccgcactgcc cagagccgtc gaaccgtcaa
     3121 agagctgcac ctgtccgttc gcgcccacgg aagcggtcac ggcaatggca aacgcgatgt
     3181 ttgtgccact agcgaccttg gtgctcgtca ccgtaatctt cg
//
LOCUS       DASYVO010000376        13738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_111671, whole genome shotgun sequence.
ACCESSION   DASYVO010000376 DASYVO010000000
VERSION     DASYVO010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 13738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13738
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111671"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3851)
                     /locus_tag="VGR03_12745"
     CDS             complement(<1..3851)
                     /locus_tag="VGR03_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV1995189.1"
                     /translation="MQRRQGVCASAASRPQVQSTATRSGLRHKLTVLQRLITVTILCL
                     LVLGWASMVSAQVNTATLSGTVSDPQNLPVKGAKVTVTNAGTGAARTSLTDDAGRYNL
                     VGLPPGRYNMTVDGGANFGVYENASLVLTVGESANFDPRLELKGMQQSVTVSAETAPI
                     ETTKSDVSQTVEQRRIDNLPINGRSYINFTLTNSQATRDMAPTIGPAPNSGLSIGGAR
                     ARGTAVSVDGADAVDNSINAIRSTLSQEGIQEFQLILSNYNAEYGRASGGVINIVSKS
                     GTNEFHGNAFGYFRNKYFQARNAFSGQVDPVTGELSPRRQAYTRVQSGLTFGVPIKKD
                     KTFFFFSYEYTQREESGFSSIGINKFGMQTVTLPTPGGPLPVQLTGPQASAVNSLLAS
                     GVPALQTLGVQYGVFMGSASSVALNGLDYGALVTTLSGGSITPVNPVPVPGGFLFQEF
                     PLPVSCPSGATVNSLVCSGFAAVGPKSSVVPAGAVPLPASFAALNGVRGNYPVMEKTS
                     LWSGRLDHHWNNRNNSFLRTGISPSLITGLPSTSQNQVFGQNAGTRAGLSQTRDLSFT
                     FQHDTIVSDTAFNEFRLQVARRGLHFGFSQLPGGSDIAVNIPGFAYFGREPYSTVDRI
                     ERRFQFTDNVTLVRGKHTFKIGGDVNVIQLRSKKAQIFELDFGGVANFGGFSASTFGF
                     PNSIAGISLPGTTGLQSYGLGIPTSYIQGIGSSNAPLDNLPFAFFLQDNWRVNRHLTF
                     NYGVRYDVEISPLFAPATAVNAAAEKAVGVVEGIPRDYNNFAPRFGLAWDPAGNGKTV
                     IRAGYGLFYDHPLLATAFDSVTADGGRSVQLLSTGGTPSACGLITAACANGLDSPGNL
                     NGSSIFQGVLNTTAVPSMFYLPGQQRFNPTAAGSLFANQNYLTAGFPLPILPFTLPLG
                     KDFVYGYAEQANLTIEREIAGSWKFSLGYQRTRGLHLNRPQDINSTDPHLLAQNAFNA
                     AASGLRVGNPLTVVVPSGKPNSCVATSASSSIFLIAPGALAEGFAGSSSCAQPNLVGF
                     IGTPAFFNFFRPSGPNPSFAGVTPGGYGTEVALATLAGYPKGFGVPVPFNSVDAQLSN
                     GNSWYNSLTFNLTKRFSKGFELLSSYTWSHSIDDSTDLQSPLEPQDSRFPNLERSNSV
                     NDQRHRWVNSAVFQSAGAKSGDGFFKHLIADFTLSPIIEYSSGRPFNVITGTDTRLDL
                     GASQARPSVGGATSSPFIPGVTFGPADVCLKNDGSTFTVPLVSPPAGCNGNLGRNRFT
                     SPNFFQVDMRLSRRIPLGERF"
     gene            complement(4278..5678)
                     /locus_tag="VGR03_12750"
     CDS             complement(4278..5678)
                     /locus_tag="VGR03_12750"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEV1995190.1"
                     /translation="MVRSVAGTKGETRGGNYVILDARTTFYVPDDRQVIVYFEWEAPK
                     GKHHCEGTVKGPNGQLAVMSSFDYPATQTKFGGFWTMPLLENSSPGLWTFESRVDGES
                     AGELRFEIVSAKRPADTAKEEAPPTPAEIYSHTVAATALIEKLDEKGQPLGAGSGFLT
                     GDGSLITAFRVIDGAHTLRIQFADGTRIQTDSIAGWNRRQDWVILKVNTGKNAVLRTA
                     DSKSWSIGDHCYWLDTKTDGGRVISDGQIVGKESRAGWGERISISNIFNSAATGGPLL
                     NERGEVLGLLGGALPDSLVPQPMRDSSADGAGGSTYTITASAIPIGLVIPPPNLTPAS
                     LQTLRANGQFTPPVTASRNMTFGMLTQGKPTKGRAPFIKEMKNDFTRQDDAASVYVAL
                     QSMDTLKTTVQMRMYDADNRVVSSGEPIKVTLRRGETQDRYWSFSVGVLNPGIYRADV
                     LLGESVAWRTYFRVRE"
     gene            complement(5970..8618)
                     /locus_tag="VGR03_12755"
     CDS             complement(5970..8618)
                     /locus_tag="VGR03_12755"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEV1995191.1"
                     /translation="METPLVLRATLPVHEAKREATSQSNPDLTPRTYLRQLATAVSRV
                     PAGATDHKAIEVHRALRNLHLLMRSERLYENNHPRRMDSLDSAYDSIRNATEILGGLE
                     IRVERGGLVAPRIGDTHMPDARGEMQALASDLQRAGIHALAFSKKFHVGELDTLARLV
                     KASLLRSEEPANGGGNAWWPARLLENRVEGISINTQTERRVDTVLASLIGALVAYGGH
                     SPRETADAPIQAPDFDDLVASLRLLARLTPPLESARGLSPEEAARAIHGAMEEASRDS
                     VTMLLSSVSQYGPREGERPQPYLLRLSEHLVFEFLSAEFSSGSLTASSVRPTICRLSD
                     VIIEAGAYSGPHSSQHLSSLAVTWATDTHREQLIDQFWLELSPREKSGVLRGPDVWCV
                     PVAALRHTLGKLADAGADAPRREARNIVLNYARRIEHADPSARRSVAAGLNELSSIFE
                     SLWPNQLPEDLSRGAMKALDMEKAPETAALLAAFVESLGRIAVNRADYSGFETILTGL
                     ERVPQDKEHDHMAALANRLVAQDRWLLLVDAALANRALDPVLPRLLQRDPERLLDRMT
                     LLLTEPRGAEMVPAMARLLRTIGVPVLNLLETRLYEARRQRVTAAIKLLAVADPERLL
                     RGLARAMASWEWNLQDLAVSELSRPNNSASALSTAFVFSAILADAHPLVVPMMIDQLG
                     LAQETTAVPQLMEIAAGEHEVLRDQFVRIKAIEALGRMRASEAAELLRTLAESREGLT
                     YAEPAGLRAAAEDALAMIENRPSFKQAAAAFGAPGQSSSGYVIPRRYTRVPLDSPLRA
                     HIEGAPAGLARVKTISLGGAYLESPRKLSIGDSIKLEVRAGLRKINFTAVVRNIGPDG
                     SGVEFVHMRDEDREKLRKLVQRHLQL"
     gene            complement(8904..11201)
                     /locus_tag="VGR03_12760"
     CDS             complement(8904..11201)
                     /locus_tag="VGR03_12760"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV1995192.1"
                     /translation="MMPTVTKAGRYEIVGELGRGAMGVVYKAMDPVIGRTVAVKTIRL
                     SEEGTGLSRPELLTRFQTEARAAGLLTHPNIVVVFDAGEEDGLYYITMELVEGKSLQA
                     LLDGGHAFPLPRTLRIMEQTCSALQFAHERNVIHRDIKPANLMLTADDTVKVTDFGTA
                     KILQFGTVQQTAHVMGTPSYMSPEQVKGRAVDGRSDIFSLGVMLYEMVTGEKPFPGQN
                     ITTVIYKIVNEDPVPPRQIDPSIHPGISAVVMKALVKEPDQRYQSCREMLEDLRNYRS
                     IAPGSGSPQSTMAMGGGSPAATLVSGNAAGRGLTGEDQTVVATARSLNARASAPGQTP
                     VVRRTGMIAPAPEPPKKKNVLGTILAALLLLGVIVYGANKIRPVFEAARELHDEQKKG
                     GSLPAAVPAASESTPASSAAGSSGSETSTQPKDPAAGAGAEPKAAESKPAEIVAEKVA
                     PRKPENSISPKAAEYKGRIEEAISERGLSGHVKVQAAGNTLTLAGKLRPTEHGALLKL
                     LRNAPSDVRVIDHLEYDDTPVVAPGSPDEGTHPVPTAGRGAIHVVTDVIGATAVLHGP
                     AGHILNKCETPCSFNNLSPSRYSLEVQKDGYQSVQTALLVKSGDAQDQKINLESLAKG
                     IYISSQPRGADVFINGAKQSGQTPVTLPLATGQYNLVLRLQGYEPYAGGIQVKDNIQT
                     LLNVPLSEKSASRVAWAQVNTNPKGAEIIVDGTSTGQFTPARVQVPTGLHTVTLRLTG
                     FQQAKRTVQVSEGGTVTIDESLHER"
     gene            complement(11706..12023)
                     /locus_tag="VGR03_12765"
     CDS             complement(11706..12023)
                     /locus_tag="VGR03_12765"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEV1995193.1"
                     /translation="MKRIILLVAMVVLAAGVAFAHGKEQHVMGKVTAITDSSITVQTK
                     AKEPVTVYTMAETKYEKSGTAASMKDLKVGDRVVIHAEKMGDKLMANEVHFGVVAKTP
                     QQR"
     gene            complement(12039..12641)
                     /locus_tag="VGR03_12770"
     CDS             complement(12039..12641)
                     /locus_tag="VGR03_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV1995194.1"
                     /translation="MRKRTLLLSITAAGVAGAAAFGWMTIRRGFSARDNPSVLEAYLA
                     KTARNLSIPASERDAKNPFAPTPEVLREARAHFADHCATCHGNDGNGKTAIGQNLYPK
                     SPDLRQSESQNLTDGQIYYIIHNGIRLTGMPAWGRPSKDDDSWKLVLFIRHLPQMTPQ
                     EMKDMEHFNPKSAADRSEEENEQRFLDEGKTPEMKMRMHH"
     gene            complement(12652..>13738)
                     /locus_tag="VGR03_12775"
     CDS             complement(12652..>13738)
                     /locus_tag="VGR03_12775"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV1995195.1"
                     /translation="SRFVLRELNGHQPMPWRDESRLREAIGHIAPFVVEEQKLRVVTN
                     VRMSYIQGLAAQQTLELRQNLSKLADDAVETSHQLANVGQADAPDVLESEVEAQQAQL
                     VVAMAEQNQQRVWKALAAVVGNPRLPLMRLEGKLEDTAPVDANELVERIVNESPAVRI
                     AELGVKRAEAALARAKREPIPDLQLRGGMQQNGELLSEPSGRPVGLQGFVDVGVRIPI
                     FNRNQGNIATAKADAERAKREVERVKLVLRERAASVVQTYTFSQTAVDRYKNQMIPRA
                     QKAYEMYTKKYQSMAAAYPQVLIAQRTLMQLEVSYITALENFATSSLSLESYLLMDGL
                     EAPSQPVGIDQPVREVNIPFQMGTNPR"
BASE COUNT         2686 a         3919 c         4198 g         2935 t
ORIGIN      
        1 ttgaatcgct cgccgagggg aatgcgccgg gagaggcgca tatccacttg gaaaaagtta
       61 ggcgatgtga agcggttgcg gccgagattt ccgttgcagc ctgctggggg agaaacgagg
      121 ggcaccgtaa acgtcgagcc atcgttcttg agacacacgt cggcaggacc gaaggttaca
      181 ccggggatga agggcgacga ggtggccccg ccaacggaag gccgagcctg ggacgcgccc
      241 aaatccagtc gcgtatccgt gccagtaatg acattgaagg gccggccgga agagtattca
      301 atgatggggg acagagtgaa atcggcgatg agatgcttga agaaaccatc accagacttc
      361 gcgccggccg attggaagac ggcgctgttc acccagcgat ggcgctggtc gttcactgaa
      421 ttggaacgct ccaggttggg gaagcggctg tcctgaggct cgaggggaga ctgtaggtcg
      481 gtcgagtcgt cgatggagtg cgaccaggtg tagctggaga gcagctcaaa gcctttggag
      541 aaacgcttcg tgagattaaa ggtcaaagag ttgtaccagg agttgccgtt ggaaagttgc
      601 gcgtcgacgc tgttgaaggg aacgggaacg ccgaagccct ttggataacc agccaaggtt
      661 gccaaagcca cttctgtgcc gtaaccaccg ggagtcaccc cggcgaagga aggattggga
      721 cccgaaggcc ggaagaagtt gaagaacgcc ggtgttccga tgaaaccgac cagatttggc
      781 tgggcgcagc tactggaccc agcaaagcct tccgcgagag cgccgggagc gatcaggaaa
      841 atactggaag aagcactcgt cgccacgcac gaattaggct tgccactagg gacgacaacc
      901 gtcagtgggt tgccgaccct gaggcccgag gcggcggcgt tgaacgcgtt ctgcgcgagc
      961 agatgaggat cggtggaatt aatgtcctgc gggcggttca agtgcagtcc gcgagtccgc
     1021 tggtaaccaa ggctgaattt ccacgagccg gcgatttcac gttcgatggt gagattcgcc
     1081 tgctccgcgt agccgtagac gaaatcttta cccaaaggaa gcgtaaacgg cagaatggga
     1141 agcgggaagc ccgcggtcaa atagttctgg ttggcgaaaa gcgaaccggc ggcagtagga
     1201 ttgaagcgct gctgaccagg caagtaaaac atgctcggga ccgcggtcgt gttgaggacg
     1261 ccctggaaaa tggaagagcc gttgaggttg ccaggagagt ccaagccgtt ggcgcacgct
     1321 gcagtgatga gcccgcaagc ggacggcgtg ccgccggtag agagcagttg cacggagcgg
     1381 ccaccgtcgg cagtcacgga gtcgaaggcg gtggccagca atgggtgatc gtagaagaga
     1441 ccgtagccgg cacggatgac ggtcttgccg ttgcccgccg gatcccaagc caagccaaag
     1501 cgcggagcga agttgttgta gtctcgcggg atgccctcga cgacacccac agccttttcc
     1561 gccgcggcat tgaccgcggt ggcaggggcg aaaagcggac tgatttcgac gtcgtaccgg
     1621 acgccgtaat tgaaggttag gtggcggttc acacgccagt tgtcctggag aaagaaagcg
     1681 aaggggaggt tgtcgagagg cgcattggaa ctgccgatgc cctggatgta ggaagtaggg
     1741 attccgaggc cgtaggattg caagccggtc gtgcccggca aggagattcc ggcgatgctg
     1801 tttggaaaac cgaacgtgga agccgaaaat ccgccgaagt tcgcgacacc gccgaaatca
     1861 agttcaaaaa tctgcgcctt tttggagcga agttgaatga cattgacgtc gccgccgatc
     1921 ttgaaagtat gtttgccgcg gacgagggtc acattgtcgg tgaattgaaa gcgccgctcg
     1981 atgcgatcga cggtggagta aggttcgcgt ccgaagtacg caaaccccgg tatattcaca
     2041 gcgatatcgg agccgcccgg aagctgcgaa aaaccgaagt gcagtccgcg gcgagcgacc
     2101 tgcaggcgga attcgttgaa agcggtgtcg ctgacgatgg tgtcgtgctg aaaggtaaaa
     2161 ctgaggtcgc gcgtttggct cagaccggcg cgggtaccag cattttgccc gaacacctgg
     2221 ttctgtgagg tggacgggag gccggtaatg agagagggcg aaatgcccgt gcggaggaag
     2281 gaattattgc ggttgttcca atgatggtcg agacggccgg accacaaact ggttttctcc
     2341 atcaccggat agttgccacg aacaccgttg agtgcggcaa aggatgccgg caaggggacg
     2401 gccccggcgg gcactacgga actcttggga ccgactgcgg cgaaaccgct gcaaacgagg
     2461 gaattcaccg tggcgccgct cggacacgat accgggagcg ggaattcttg gaacaggaat
     2521 ccccccggca cgggaaccgg attcaccgga gtgatcgagc ctccggacag agttgtcacc
     2581 aaggccccat agtccagtcc atttagcgcg acgctcgagg cggagcccat gaacactccg
     2641 tactgcacgc cgagagtctg gagagcaggg acaccggagg cgagcagtga gttcactgcc
     2701 gaggcctgcg ggcccgtcag ttgcactggt agcggtccgc caggggtcgg cagagtgacc
     2761 gtctgcatcc cgaacttgtt gatgccaatg ctggagaatc cggactcctc gcgttgcgtg
     2821 tattcataag aaaagaagaa aaaggtttta tctttcttga tggggacgcc aaaggtcaaa
     2881 ccgctctgga cgcgggtgta ggcttgcctg cgcggactga gttcgccggt gacaggatcg
     2941 acttgaccag aaaaagcgtt gcgcgcctga aagtacttgt tgcggaagta cccgaaggcg
     3001 ttgccgtgaa attcgttggt gccgctcttg cttacgatgt tgatgacacc gccgctggcg
     3061 cggccgtact cggcgttgta gttgctcaga atgagctgaa actcctgtat gccctcttga
     3121 gagagggtcg agcgaatggc gttgatagag ttgtcgacgg catcggcgcc gtcaacactg
     3181 accgcggttc cacgggcgcg cgcgccgccg atgctcaagc cgctgttggg tgcggggccg
     3241 atggtcgggg ccatatcgcg ggtggcctgc gaatttgtaa gcgtgaagtt gatatagctg
     3301 cggccgttga tagggagatt gtcgatgcgg cgctgctcga cggtttgcga gacatcggac
     3361 ttggtcgtct cgatgggggc ggtttccgcc gataccgtca cagactgttg catgcctttg
     3421 agttccagac gcggatcgaa gttggcgctc tcgccgacgg tgagaacgag ggaagcattt
     3481 tcatatacgc caaagttggc gccgccgtcc acggtcatat tgtagcggcc cgggggaagc
     3541 ccgacgaggt tgtagcgtcc ggcgtcgtcc gtgagggaag tccgcgcggc gcccgtgcca
     3601 gcgttagtca ccgtgacctt tgcgcccttg accggcagat tctgcggatc agaaaccgtt
     3661 cccgaaagag ttgcggtatt gacctgtgcc gagaccatcg aggcccaccc tagtaccagc
     3721 aagcacaaaa tcgttacggt tataagccgc tgcaagaccg tcaatttatg acgcaaccct
     3781 gaacgggtcg ccgtcgattg aacctgcggc cgcgaagcgg ccgacgcaca cacaccctgt
     3841 ctgcgttgca tgagtcctcc tattgtggca ccaacgaatc ttccgctacc cctagcggtg
     3901 gcgggctatc ccgccgcttc ccagatgaga caattgaata cagggctttt actgcaatat
     3961 agcgttgctt tcaactgaaa acttccaagc cgtcgcccac gccgtcaccc gcaacgagcc
     4021 atgaagcagg gcgcagcccc tagggcgctc aaaatactga cttgactgcg tcgagagcgc
     4081 cgcggaccag cgcagaatca actagaccgg cgggattagc aagagacaag gtacgaaacg
     4141 gggtcggagc gagggaatgt cgcttcttgt cgctatctct gtgcccaccg tggattaagg
     4201 actttggtcc ctaatcccta tccccctgca gactttctgt tgactttaga tttagataga
     4261 gatgaaagag cgctgattca ctctctcact cgaaaataag ttctccaggc cacagattcg
     4321 cccaacaaca cgtccgccct gtatattccc gggttcagca ctcctacgga gaaactccag
     4381 taccgatcct gggtctcgcc acgcctcaag gtgactttga tgggctctcc gctgctaacc
     4441 acgcgattat cagcatcgta catgcgcatt tgcaccgttg tcttcagggt gtccatcgat
     4501 tggagagcga catacactga tgccgcgtca tcctgacgcg taaaatcgtt tttcatctcc
     4561 ttgatgaacg gagctctgcc tttcgtgggc ttaccctggg ttagcatccc aaacgtcatg
     4621 ttgcgcgatg ctgtcacggg cggcgtaaat tgaccgttcg cccgcaaagt ctgaagtgat
     4681 gcaggggtta aattcggcgg cggaatgaca aggccaatgg ggatagccga ggcggtaatg
     4741 gtgtaggtcg atccgcctgc gccatcggcc gaagaatctc gcatgggttg ggggacaagg
     4801 ctgtccggca acgcgcctcc cagcaggcct aggacttccc cccgttcatt caagagcggg
     4861 ccgcccgtgg cagccgagtt gaagatgttc gatatgctta tcctctcgcc ccagcctgcc
     4921 cgggattcct ttcccacaat ttggccgtca gagatgaccc tcccgccgtc ggtcttcgtg
     4981 tcgagccaat agcagtggtc tccaatcgac caggacttgc tgtcggccgt tcgcaataca
     5041 gcattcttgc cggtgttcac cttcaaaatg acccaatcct gacgccgatt ccaaccggca
     5101 atgctgtcgg tctgaatgcg ggttccgtcc gcgaattgaa tccgcaaggt atgcgcccca
     5161 tcgatgactc ggaaggccgt aatgagggaa ccatctcccg ttaaaaagcc ggagcccgcg
     5221 ccgaggggct gacctttttc gtcgagtttc tcaatgagag cagtggctgc gacggtgtgg
     5281 ctgtaaattt cagctggtgt aggcggtgct tcttcctttg ccgtgtccgc cggacgtttt
     5341 gccgacacga tctcgaatct tagttcgcct gcggattctc cgtccacgcg tgactcaaaa
     5401 gtccataagc ccggggaaga attctctaga agcggcatcg tccaaaatcc cccgaatttt
     5461 gtctgcgtgg ctggatagtc gaagctggac atcacggcga gttgtccatt tggaccctta
     5521 accgttcctt cgcagtgatg cttacccttg ggcgcttccc attcgaaata aacaatcacc
     5581 tggcggtcat ctggaacgta gaaagttgtg cgtgcgtcca gaatgacata gttcccgcca
     5641 cgcgtttcac ctttagtgcc cgccacggac cggaccatgc gaaccttcgg ccctgccgcc
     5701 tgcactccgc cggcctgttt cgcatcaagg gcagcacagt tgaaaaccaa tccacagacg
     5761 agagcgagga agaatgttcg acatctggcc gctgcctgcc aaacattttt ttccgattgc
     5821 cgagggcaca tggatttcga gtcctccatc agggaagttt cgagtgtccg ccattgtact
     5881 agacatggac ttcatccacg gagaaagccg cggaccgtcg agttgaaaaa tggagatgag
     5941 gagcgaagct gaaccgaaga atgcaggcgc tagagctgga gatggcgctg gacgagtttg
     6001 cggagttttt cgcggtcttc gtcgcgcatg tgcacgaatt cgacgccgct gccgtccggg
     6061 ccgatattgc ggacaacggc ggtgaaattg attttgcgga gaccggcgcg gacctcgagc
     6121 ttgatggagt cgccgatact gagtttcctg ggggattcca gataggcgcc gcccaggctg
     6181 attgtcttta cccgagccag gcccgctggc gcgccctcga tatgcgcgcg aagcggcgag
     6241 tccagtggaa ctcgagtgta gcgtcgcggg ataacgtagc cggagcttga ctgtcctggc
     6301 gcgccaaacg ccgcggcagc ctgcttgaac gacggacgat tctcgatcat cgccagcgca
     6361 tcttccgctg cagcgcgcaa tccggcaggt tccgcgtagg tgaggccctc acggctctcc
     6421 gccaacgtgc gaaggagctc ggcggcttca ctggcgcgca tgcggccgag cgcttcaatg
     6481 gccttgatgc gcacgaactg gtcgcgcaaa acctcatgtt caccggcggc gatttccatg
     6541 agttgcggca cggcggtggt ttcctgcgcc aagccaagtt ggtcgatcat catgggaacg
     6601 accaggggat gcgcgtcggc gaggatagcg gagaagacga aagcggtgct cagcgccgat
     6661 gcggaattgt tgggccgcga gagttcgctg accgctaagt cttgaaggtt ccattcccaa
     6721 ctcgccatgg cacgtgcgag gccccgtagc agacgctcgg ggtccgccac agccaaaagt
     6781 ttgatggccg cggtcacgcg ctggcggcgg gcctcgtaga gccgcgtttc gagaaggttt
     6841 agaacgggca cgccgatcgt ccgcagaagc cgcgccatcg ccggtaccat ttccgcgccg
     6901 cggggttcgg tgaggagcaa cgtcatgcga tcgagcagcc gctcgggatc gcgctgcaag
     6961 agacggggca ggactggatc cagagcgcgg ttggccagag cagcatctac gagcaacagc
     7021 cagcgatcct gcgccacaag gcggtttgcc agcgccgcca tgtgatcgtg ctctttatcc
     7081 tgaggaacgc gctccagacc ggtcaggatg gtttcgaaac cggaatagtc ggcgcgattc
     7141 acggcaatgc gcccgaggga ttcgacgaag gccgcaagca gggcggcggt ttcgggagcc
     7201 ttttccatgt ccagagcttt cattgctcca cggctcaggt cttccggcaa ttgattcggc
     7261 cagagggatt cgaatatcga agacagttca ttgagtccgg cggccacgga gcgtcttgct
     7321 gaagggtcag catgttcgat gcggcgcgca taatttagca cgatgttgcg tgcttcgcgg
     7381 cgaggagcgt cggcgcccgc atcggcgagt ttgccgaggg tatggcgcag cgctgcaacc
     7441 ggaacgcacc acacatccgg gccgcgcaag acacctgatt tttcgcgggg cgaaagttcc
     7501 agccagaatt gatcgatgag ctgttcacgg tgcgtgtccg ttgcccaggt gaccgccagc
     7561 gacgagagat gctgcgaaga gtgcgggcca gaataggcgc cggcctcgat gatgacatcg
     7621 ctcaagcgac agatcgtcgg ccttacggac gaagcggtga gggatccgct ggaaaactcc
     7681 gcgctgagga attcaaaaac gagatgttcg gagaggcgca acaaataggg ttgcggccgc
     7741 tcgccttcgc gcggtccata ctgggaaacg gaactgagca gcatggttac gctgtcgcgg
     7801 ctggcttctt ccatcgcgcc gtggatcgcg cgcgctgctt cttccgggga aagaccgcgt
     7861 gcgctttcta gaggcggagt cagccgtgcc aggagccgca ggctcgccac gagatcgtca
     7921 aagtccggcg cctggatggg cgcatccgcc gtctcgcgcg gggaatgtcc cccatacgct
     7981 acgagcgctc cgatgagact tgccagtacc gtgtccaccc tccgctccgt ttgcgtattg
     8041 atggaaatgc cttccacgcg gttctccaga agtcgcgccg gccaccaggc attgccccca
     8101 ccgttcgccg gctcttccga tcttagcaac gatgccttca cgaggcgtgc cagcgtgtcc
     8161 agttcgccta catggaattt tttcgagaac gccagcgcgt ggattccggc gcgctgcagg
     8221 tcgctcgcca gcgcctgcat ttctccacgc gcgtccggca tgtgggtgtc accgattctc
     8281 ggcgccacca acccgccacg ttccacgcga atctccagcc ctcctaaaat ttcggttgcg
     8341 ttgcggatgg aatcgtaagc actgtccagg ctatccatcc tgcgagggtg attattctcg
     8401 taaaggcgtt ccgagcgcat gagcaaatgc aggtttcgga gtgctcgatg aacttcgatg
     8461 gctttgtggt ccgttgcgcc tgctggaacg cgggagaccg cagtcgcaag ctgacgcagg
     8521 tacgtacgcg gagtaagatc cgggttcgac tgcgacgtcg cctcgcgctt tgcttcatgg
     8581 accggcaatg tggccctgag gaccaaagga gtctccactt gcttccttaa agttcgttca
     8641 ccccgcatcg ggcgacaagg gggttcgcct tcactctcgc aatgaactcg cgtgtggacg
     8701 ggcggtcccc acccgtaaag caagcacgcc tccggcctca tacggacggt gctaagtcct
     8761 ttgtctgtag cgaatactat cgatggttct agaactctat gtgatcttat gtaacaaaat
     8821 gttacgtttt gggtagacct tctgggagtc tggtggaggg agacggtcgg actaatttgc
     8881 gggattcttg aaggatgtac tctctatctc tcgtggagcg actcgtcaat cgtgaccgtg
     8941 ccaccctcgc tcacttggac cgtcctcttc gcctgctgaa atccagtcaa acgcagcgtc
     9001 accgtgtgca acccggtggg cacctgcacg cgagccggtg tgaactgtcc ggtggaggtc
     9061 ccgtccacga tgatttccgc gcctttcgga ttcgtgttta cctgcgccca ggcgacgcgg
     9121 cttgcggact tttcgctgag cggcacgttc agtagcgtct ggatattgtc cttgacctga
     9181 atgcctccgg cgtaaggctc ataaccctgc agccgcaaca cgaggttgta ctggccagtc
     9241 gcgagcggaa gcgttacggg tgtctgtccc gattgtttcg ctccgttgat gaagacgtcg
     9301 gctccccgcg gctgactgct gatgtaaatg cctttggcga gcgattcgag attgatcttc
     9361 tggtcctggg cgtcgccact ctttactagc agcgcggttt gaacggattg ataaccgtcc
     9421 ttctgcacct caaggctgta gcgtgacgga gacaaattgt taaagctgca aggcgtttcg
     9481 cacttgttta ggatgtggcc tgcaggcccg tgcagcaccg cggtagcgcc gatcacgtcg
     9541 gtaacgacat gaatcgctcc gcgccccgcc gtaggaaccg gatgagtgcc ctcgtcaggg
     9601 ctgccggggg ccaccaccgg tgtgtcgtcg tattcgaggt gatcgataac gcggacgtcc
     9661 gaaggggcgt tccgcaataa tttcaagagt gcgccatgtt cggtcggccg gagctttccc
     9721 gcgagcgtca gcgtgttgcc ggcagcctga actttcacat ggccgctcag tcctctttcg
     9781 gaaatggcct cttcgatccg gcctttgtat tccgccgctt tggggctaat cgaattttcc
     9841 ggtttcctgg gcgccacttt ttctgcaacg atttccgcgg gcttggactc ggccgctttc
     9901 ggctccgcac ctgctcctgc cgctggatcc ttcggttgcg tagaagtctc gctcccagag
     9961 gaacctgccg ccgagctggc cggtgtgctt tctgatgccg ccggcaccgc agcgggcaag
    10021 ctgccaccct tcttctgttc atcatgaagc tcgcgtgcag cttcaaagac tggtcttatc
    10081 ttgttcgccc cgtaaacaat cacgcctaga agcagtaacg ccgcgaggat cgttcccagg
    10141 acatttttct tcttcggagg ttccggggcg ggtgcgatca ttcccgttct ccgcacgaca
    10201 ggtgtttgtc cgggagccga tgcccgcgcg ttcaaggagc gcgccgtcgc tacgacggtt
    10261 tgatcctcgc cggtgaggcc acggcccgcc gcatttccgg aaaccaaggt cgccgcgggc
    10321 gagcctccgc ccatggccat cgtcgactgt gggctcccgc tgcccggcgc gattgaccgg
    10381 taattccgca gatcttcgag catttcgcgg caactctggt atctctggtc gggctctttc
    10441 accagcgcct tcatgaccac cgcactgatg ccgggatgaa tcgaaggatc aatctgtcgc
    10501 ggcggaacgg gatcttcgtt gacgatctta tagatcaccg tggtgatgtt ctggccagga
    10561 aaaggttttt cgccggtcac catttcatag agcatgacgc ccagcgagaa aatgtcgctg
    10621 cggccatcga cggcgcgccc cttcacttgt tccggcgaca tgtagctggg ggtgcccatg
    10681 acgtgcgcgg tttgctggac cgtgccgaac tgcagaatct tcgccgtgcc gaagtccgtg
    10741 actttgactg tatcgtcggc ggtaagcatc aggttcgccg gcttgatgtc tcggtggatg
    10801 acgttgcgtt catgcgcgaa ttgcagcgcg ctgcaagttt gctccatgat gcgcagcgtg
    10861 cgcggcagcg ggaacgcgtg gccgccgtcg agcagcgcct gcaggctctt tccctcaacc
    10921 agctccatgg tgatgtagta gaggccgtct tcttcgccgg cgtcgaaaac caccacgatg
    10981 tttggatgcg tcagcaaacc tgcggcgcgc gcttcggttt ggaagcgcgt caggagttcg
    11041 gggcgcgaga gacccgtgcc ttcttcgctc agccggatgg tcttgaccgc gacggtgcgc
    11101 ccgatcaccg ggtccatggc tttgtaaacg acacccatcg cgccgcggcc cagttcaccc
    11161 acgatttcgt atcttccggc ttttgttact gttggcatca ttcaatcgat ctgcaaccca
    11221 gcgagggcag cgctacacac acaaacccaa atggcgcgaa tcgggttgcg gttctggaag
    11281 gaacagaatc tggaaatacc cccagaccat ctatcggcga tttcctgatc tcgatcagcg
    11341 gaaatgcctc cgccgacacc cgggcgccct atcctgcaca gtaccgagtg agttttctgt
    11401 tggtcaacgc tactggggta ctagtgttgc gcactcgata cggtcggaat gcggtgttca
    11461 ggcagttcct cttcttaaca tacccccgca cttgccgggt tggcgtggcc ctggcgcaat
    11521 ttaggggttg cgcgcccatt gtccggagcg acatcgtgcg ttccgccatc ggcacgttgg
    11581 tttcgcggat gggacgatcg acttcgccgg ggcgtgcggg agcttcgagt ccatccgtga
    11641 gcagatatcc ttgcagcgct atgccgttcg tccagacgct ctcgagcgcg ggttcgaaaa
    11701 cttgcttatc gctgctgagg agttttggcc acgacgccga agtgcacctc attcgccata
    11761 agcttgtccc ccattttttc ggcgtggatg accacccggt cgcccacctt gaggtccttc
    11821 atcgaagcgg ctgtaccgct cttttcgtac tttgtttcgg ccatggtgta gacggtgacg
    11881 ggttccttgg cttttgtttg caccgtaatg gaactgtccg tgatggccgt caccttcccc
    11941 atgacgtgct gctctttgcc gtgggcaaac gcaacccctg cggcgagcac aaccattgcg
    12001 accaacagaa taattcgttt catttttgtt ctccgttttt agtgatgcat cctcatcttc
    12061 atttctgggg tcttgccttc atccaaaaat cgctgttcgt tttcttcctc tgagcgatct
    12121 gccgcgctct tcgggttaaa atgctccata tccttcatct cttgcggagt catctgaggc
    12181 aggtgccgga taaacagaac cagcttccag ctgtcgtcgt ctttgctggg gcggccccac
    12241 gcgggcattc ccgtaaggcg gattccgttg tggatgatgt aataaatctg cccatcggtg
    12301 aggttctggg attcggactg gcgcaggtcc ggtgactttg gatagaggtt ctgtccgatc
    12361 gccgttttcc cgttgccgtc attgccgtgg catgtggcac agtggtccgc gaagtgggcg
    12421 cgcgcctctc ggagaacttc aggggttggc gcaaacgggt tcttcgcatc ccgctcggac
    12481 gcaggaatgg aaagattgcg tgccgtcttt gcgagataag cctctagcac agacgggtta
    12541 tcgcgtgcgc tgaagccgcg ccgaatggtc atccatccga acgcagcggc tcccgcgacg
    12601 ccagcagcgg tgatcgatag aagaagagtg cgtttcctca tgtgctcacc tttatcgggg
    12661 gtttgtgccc atctggaatg ggatgttgac ctcacgcacc ggttggtcga ttccgaccgg
    12721 ctgcgacgga gcttctaagc cgtccatcag caagtaggac tccagagaaa gtgaactcgt
    12781 cgcgaagttc tccagcgcag tgatgtagga gacttctagc tgcatcaggg ttctctgggc
    12841 gatgaggacc tgtgggtaag ccgcggccat gctctggtat ttttttgtgt acatttcgta
    12901 tgccttttgg gctctcggta tcatctggtt cttgtaacgg tcgaccgcgg tttgcgagaa
    12961 cgtgtaggtc tggacgacgc ttgccgcccg ttcgcgcaag acaagcttca cgcgctccac
    13021 ttcgcgcttc gccctctctg catcagcctt agcggtggca atgtttccct ggttgcggtt
    13081 aaaaatggga attctcaccc ccacgtcaac aaagccctgc agtccgacag gtctcccgct
    13141 aggctcggac aacaactcgc cattctgctg catgccaccg cggagttgca ggtcgggaat
    13201 cggttcccgc ttcgcgcgag ccaaggctgc ttccgctcgt ttgacgccga gttcggcgat
    13261 tcttacggcc ggactctcgt tgacgatcct ctccaccagt tcattcgcgt ccaccggcgc
    13321 agtgtcctcg agttttcctt cgagccgcat caagggaagt cgcggattcc ccacaacggc
    13381 ggccagtgct ttccaaaccc gctgctggtt ctgttcggcc atggcgacca cgagttgcgc
    13441 ctgttgcgcc tccacttccg attccagaac atcgggagcg tccgcttgac cgacattcgc
    13501 gagttgatgg gaggtttcga cagcatcgtc ggcaagcttg ctgaggtttt gccgcaactc
    13561 cagcgtctgc tgtgcagcca gcccttggat gtaggacatt cgcacgtttg tgactacgcg
    13621 cagtttttgc tcctcgacga caaacggcgc gatgtgcccg atcgcttcgc ggagtcggga
    13681 ctcgtcgcgc cagggcatcg gctggtggcc attcagctcg cgaagcacga accgagac
//
LOCUS       DASYVO010000377         3602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_55912, whole genome shotgun sequence.
ACCESSION   DASYVO010000377 DASYVO010000000
VERSION     DASYVO010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55912"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VGR03_12780"
     CDS             complement(<1..943)
                     /locus_tag="VGR03_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1995196.1"
                     /translation="MNWGFLLLRIVIGLSILTLLFLSQRFWYRALWRVTSHWGRVTLR
                     VGARLAYIVLLLLTIVTMAQGLVFGHGGFARQSSAMTVITGLWFFSALFAYLAVKTVH
                     GLERLWHLLHAATAKNFPKPHASAAASSNAIAETVADPGRRYFFRAATAAAGAAPFLG
                     AMYGFAAERLNYQVRRVEIPLPNLPPALDGMQIVQISDIHLSGYMSRTQVRRAVDMAN
                     DLGADLAVVTGDLITGTGDSIADCVDEISRLHAPLGIWGCNGNHEIYARAEDEAAYLY
                     AQAGMKLLRHENAQIIFKGASFNLIGVDYQRERTRNGQ"
     gene            1154..1723
                     /locus_tag="VGR03_12785"
     CDS             1154..1723
                     /locus_tag="VGR03_12785"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV1995197.1"
                     /translation="MKMLKWVAALALAMGLAVLPVLAQETTKKEPPKPALSPSQAIQE
                     QWNDIGRKLIAMAEDFPEDKYEYKPHPDSRSFAANLLHVSGSMYYFTDVAQGQKPRYP
                     DDPKRDELKTKAQVVAFVKKCVQDGADVIKAKGDKWLNDSVNDGSLRLVRISDLAWGL
                     MEHSGEHYGQLVVYYRNNGLVPPESRPKK"
     gene            complement(1919..>3602)
                     /locus_tag="VGR03_12790"
     CDS             complement(1919..>3602)
                     /locus_tag="VGR03_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995198.1"
                     /translation="ADSYRNKRAELERAANSLADAVSQAEVFTGARADFDLGVVDAQI
                     KSITQLFDIKNGGFGRAPKFPHSSAIDLVLERYQQTKEKHLLAMAETTLEKMARGGVY
                     DQLAGGFHRYSVDERWLVPHFEKMSYDNSELLKNYLHGWQVTQNPFLRETAEGIIGWV
                     NQVLSDQENGGFYASQDADYSLDDDGDYFTWSLEELRAALLPDEARVMELYYDVEVHG
                     EMHHNPAKNVLWMARDATDIAKQLGLDETTARLTIAKAKGKMLAARIPRPTPFIDKTM
                     YVSWNAMFVSAYLDAARVLGGSLGASCRAFALKTLDRMLKEVWNETRGFGHRIGGPAL
                     EGSLDDQVFSVLALLDAYEATLDPRYFTAAQKTMDLAIARYGDAEGGGFFDRPSDAAP
                     MGGLDVRRKPFQDSPTPSANSVAAISLIRMHAFTGDDRYHVFAQKTLEAFAGIAPQYG
                     LFAATYGLAATLFANHPLQVVITGDLNDPAAQALEAAAHRVFRLGKSVLRVTPETPQL
                     HLAGALKETLPHLPPDKALAVVCSGQTCLSPTSDPEQLIALLENGATGTAAS"
BASE COUNT          764 a          880 c         1262 g          696 t
ORIGIN      
        1 tctggccgtt gcgagtacgt tcgcgctgat aatcgacgcc gatgagattg aatgaggcgc
       61 ccttgaaaat aatctgagcg ttttcgtggc ggagcaattt catgcccgcc tgcgcgtaga
      121 gataagccgc ttcgtcttcc gcgcgcgcgt agatttcgtg attgccgttg caaccccaaa
      181 tgccgagcgg agcgtgaaga cggctaatct cgtcgacgca atcggcgatg gaatcgcccg
      241 ttccggtgat gagatcgccg gtgacgacgg caagatcggc gccgagatca ttggccatat
      301 ccacggcgcg gcgaacttgt gtgcgggaca tgtagccgct gaggtgaatg tcgctgattt
      361 gcacgatctg catgccgtcg agagcgggcg gcaaattggg caagggaatc tcgacgcggc
      421 ggacctggta attcagtcgc tccgcggcga aaccatacat ggcgccgagg aaaggagcag
      481 cgccagcggc agccgtggcg gcgcggaaaa aatagcggcg gcccgggtca gcaaccgtct
      541 cggcaatggc gtttgaggaa gctgccgcag aagcgtgtgg cttagggaaa ttcttcgcag
      601 tggcggcatg cagcaaatgc caaagccgct ccaaaccatg aacagtcttc actgcaaggt
      661 aggcgaagag cgcgctgaaa aaccagaggc cggtgatcac ggtcatggcg gagctttggc
      721 gggcgaagcc tccgtggccg aagaccaagc cttgagccat ggtaacgatt gtcaggagaa
      781 gaagaacaat gtatgcgagc cgggcgccga ctcggagcgt tacccgaccc caatgcgagg
      841 tgacacgcca aagagcgcgg taccagaagc gctgtgagag aaacagcagc gtcaggatgc
      901 tcaaaccgat gacaatgcgc agcagcagaa agccccaatt cacggtgatg gcgcccccaa
      961 gtacgaacta taagcttacc atgcgggggc gggaattttg catgacgggg gcgattttac
     1021 tgcgtaagat gaacccccgc ggaaggccgg gtgctgcaat cccagtgaag cctgatttgg
     1081 tgaaacgcct ggcagagtgg tgcgtctgaa ggagagaacg tccggcttcc gggcgtaacg
     1141 gaggaaagaa aaaatgaaga tgcttaagtg ggtggcagca ttggctttgg cgatggggtt
     1201 agcggtgctg cctgtactgg cgcaggagac gacaaagaaa gaaccgccaa aaccggcgct
     1261 gagtccgtcg caggccattc aggagcagtg gaacgatatc gggcggaagc tgatcgccat
     1321 ggcggaggat tttccagagg acaagtacga atacaaaccg catccggatt cgaggtcgtt
     1381 cgcggcgaac ctgctccatg tttccggttc gatgtactac ttcacggacg tcgcgcaggg
     1441 acagaagcca cggtacccgg acgatccgaa gcgcgatgaa ttgaaaacca aggcgcaggt
     1501 ggtggcgttc gtgaagaagt gcgttcagga cggcgcggat gtgatcaagg cgaaaggtga
     1561 caagtggttg aacgatagcg tcaatgacgg cagcctgcgg ttggtgcgta tctcagactt
     1621 ggcgtggggc ttgatggagc attcgggcga acactacggg caactggtgg tgtattaccg
     1681 gaataacggg ctggtgccgc cggagtcgag accgaagaaa taagggcaac tggtgactag
     1741 tgactcgtgg aaagccgcca gtcgcgagtg agattcaaaa cagttgttcc cgggcgcggt
     1801 gcgcgagcat cgcgcccggt tttgttttaa aaagaaaatc gaattggcct tctgggctgc
     1861 atacgtggtg gtaggggcgc agcatgctgc gcccctacgc caggaagtcc tgagcatgtt
     1921 aagaggcagc tgttccggtg gcgccgtttt cgaggagggc gatgagttgt tcgggatcgc
     1981 tggttggcga gagacaggtt tggcctgagc agaccacggc gagtgctttg tcgggcggga
     2041 gatgagggag agtttctttg agggcgccgg cgagatggag ttggggagtt tcgggtgtga
     2101 cacgtagaac ggatttgccg aggcggaaga cgcggtgggc ggctgcttcg agggcttgcg
     2161 cggcgggatc gttcaaatcg ccggtgatga ctacttgcag cggatgattc gcgaagagcg
     2221 tggcggcgag gccgtaggtg gcggcgaaga ggccatactg cggggcgatg ccggcgaagg
     2281 cttcgagcgt tttctgggcg aagacgtggt agcggtcgtc gccggtgaag gcgtgcattc
     2341 gaatcaagga gatggcggcg acggaattgg cgctgggcgt gggagaatcc tgaaagggct
     2401 tgcggcgaac atcgagaccg cccatgggcg cggcgtcgga gggacgatcg aagaagccgc
     2461 cgccttcggc gtcaccgtag cgggcgatgg cgaggtccat ggttttttgc gcggcggtga
     2521 agtagcgggg atcgagcgtg gcttcgtagg cgtcgagaag cgcgagaacg ctgaaaactt
     2581 gatcgtcgag cgagccttcg agggcggggc caccgatgcg gtggccgaag ccgcgggttt
     2641 cgttccaaac ttctttcaac atgcggtcga gcgttttcag ggcgaaggcg cggcagcttg
     2701 cgcctagtga gccgcccaga acccgcgctg catcgagata ggccgagacg aacatcgcat
     2761 tccaggaaac gtacatcgtt ttgtcgatga acggtgtggg acgcgggatg cgcgcggcga
     2821 gcatcttccc ttttgccttt gcaatagtca gacgggcggt ggtttcatcg agaccgagct
     2881 gtttcgcgat gtccgtggcg tcgcgcgcca tccacagaac attttttgcg ggattgtggt
     2941 gcatttcccc gtgaacctcg acgtcgtagt acagttccat gacgcgggct tcgtcgggga
     3001 ggagtgctgc gcggagttct tcgaggctcc aggtgaaata gtcgccgtca tcgtcgagcg
     3061 aatagtcggc gtcctggctg gcgtagaaac cgccattttc ctgatccgat aaaacttggt
     3121 tcacccagcc gatgatgcct tcggcagtct cgcgcagaaa tgggttctgc gtgacctgcc
     3181 agccgtggag ataatttttc aagagttcgg aattgtcgta agacattttt tcgaagtggg
     3241 ggacgagcca gcgctcatcg acggagtagc gatggaagcc gccggcgagc tggtcgtaga
     3301 ctccgccgcg ggccattttt tcgagggtgg tctcggccat ggcgagcaaa tgcttttctt
     3361 tcgtttgctg gtaacgctcg agaactagat cgatggccga ggagtgcggg aatttcgggg
     3421 cgcggccaaa gccgccgttc ttgatgtcga agagctgggt gatggatttg atctgcgcgt
     3481 cgacgacgcc gaggtcgaaa tcagcgcgag caccggtgaa gacttcggct tgggaaacgg
     3541 cgtcggcgag ggagttggcg gcgcgttcga gctcggcgcg cttgttgcgg taagaatccg
     3601 cg
//
LOCUS       DASYVO010000378         3453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_6543, whole genome shotgun sequence.
ACCESSION   DASYVO010000378 DASYVO010000000
VERSION     DASYVO010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6543"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..531)
                     /locus_tag="VGR03_12795"
     CDS             complement(43..531)
                     /locus_tag="VGR03_12795"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEV1995199.1"
                     /translation="MSDHKDRKQEEAFRVIDRRPFTAEGELRQEVVEEEAREAKREAA
                     KIPAALPEAAKAVAPPLPTETPKGLPAFENLVRMLGSNAAMVLGGYADPRTGQPMIDP
                     EAARELIDMLDALHEKTKGNLAPDEDTLLLDLLGKLKITYLEINQAVAAEAAKAKAKA
                     RP"
     gene            complement(715..3369)
                     /locus_tag="VGR03_12800"
     CDS             complement(715..3369)
                     /locus_tag="VGR03_12800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1995200.1"
                     /translation="MPLFVKVRSFLRNLFLSRRVDVDLDQEVRSHLELLVEENIRAGM
                     PPHEAQRAARIELGGVEQVKEQVRDSRTGAFLDSLLQDLRFALRQMRRSPGFALTAVL
                     ILALGIAANVIVFGVLQALVLRHLDVPHADRVMTLQPKHGGPFLAYPELRDVRDDNTV
                     FSAVAGVEVQEFGLEANGVTRHVWGYEVSGQYFEVVAIKPFLGRLLERSDDDHPGASE
                     AAVLSWPAWKSDFGADPKIVGTMVRIDKHPYTIVGVTPEGFYGTEKFTQPDIFVPMAN
                     EASLDGVNWLESRSHKNVFSIVRIKDEVTLPQAQAGLNAIAARIARQYPKDEEGLALK
                     LARPGMIGDFIGGPARGFLAGVMGLAGIVLLAACANLGSLFAARTADRTREIAIRMAI
                     GSSRWRILRQLLVEALVISIFGGACACGLAWMALTGLAGWHPPSRFPFKFQVLPQPSL
                     ILMAFLISVLAGVLFGVIPLRQIFKTDPNDAIKSGGSLSSAGRRWALRDVLLAAQIAL
                     CCVTVTAALVSLRGLGKALTMDLGFNPKNAVRTQFDLGRAGYTSDAAADHFQRQLLER
                     VSQIPGVDAAGYANTTPLSFEQSTSAVFSQQSIDFRPSKKAFDTYFYGVSPGYLTASG
                     TPLLAGRDVSFTDTAKTPAVAIVNQEFARRLFHSEHAVGRYFKNRYGVSIQIVGIMAD
                     GKHFTLSEDPEAAAFFPISQVGDTRTSLIVRTRRDTADMVATIREVVGDLDRAVPIQE
                     SGPWNSQLALSLFPSQVATVALGVFGAFGLLLSIAGTFGLASYTVSKRLRELSIRVAL
                     GAQAKQILSAALGRMLILLGSGSVVGIVLGLAASRVLSVIVYQASAQDPFVLAAVALT
                     VLLTGSLSVAGPVRHVLHIDPAILLREQ"
BASE COUNT          754 a         1008 c         1028 g          663 t
ORIGIN      
        1 accaatcgtg acgcgaagcc gggtcagacc gccggacgtt ccctaagggc gagccttcgc
       61 cttggctttg gcggcctcgg ccgctaccgc ctgattgatc tccagatagg taatcttcag
      121 cttgcccagc aggtccagga gcaaggtgtc ttcatcgggc gcaagattac ctttggtctt
      181 ctcgtgcaag gcatcgagca tgtcgatcag ctcgcgggcc gcctcgggat cgatcattgg
      241 ctggccggta cgcgggtcgg cataaccgcc gagcaccatg gcagcgttgc tccccagcat
      301 acggacgagg ttctcgaaag ccgggaggcc ctttggcgtc tctgtcggaa gtggaggggc
      361 cactgctttt gctgcctcgg gcagtgcggc tggaattttt gccgcctccc gcttggcttc
      421 ccgcgcttct tcctcgacga cttcctggcg caattcccct tcggccgtaa aagggcgccg
      481 gtcgataacg cgaaatgctt cttcctgttt acggtcttta tggtcggaca aggtgctttc
      541 ctcagcgatt gatctcgctt gcagtttatc acgggttgcc agcgcgagtc gctgcggtac
      601 gcggcccggt attgggtcat ttttcgatta acgtgttaat caggcatgcg gattacttcc
      661 gggtgcgtgt cactcgggga cattcaaata ggaaggccaa atcgggctcg aaaattattg
      721 ttcgcggagc agaattgccg ggtcgatgtg caggacgtgt ctcacgggac cggcgaccga
      781 aagtgatccg gtgagcagca cagttaatgc aacagcagca agtacgaatg gatcctgcgc
      841 ggaggcttgg tagacgataa ctgagagtac tcggctcgcc gccaaaccca gcacaatgcc
      901 aaccacagag ccgcttccga gcaggatcag catacgaccg agcgctgccg agagaatctg
      961 cttcgcctgc gcaccgagcg cgacacgaat actcaactcg cgcagccgct tgctgaccgt
     1021 ataggaggcg aggccgaagg tgccggcgat cgagagcagg agtccaaaag cgccaaagac
     1081 gccgagggcg acggtagcta cctgggatgg aaagagactc agtgcaagct ggctgttcca
     1141 ggggcccgat tcctgaatag gaacggcgcg gtcaagatcg ccgacgactt cgcgaatggt
     1201 ggctaccata tcggccgtgt cgcgccgggt ccggacgatg agcgacgtcc ttgtgtcccc
     1261 tacctgcgag atcgggaaga aggctgcggc ttccggatct tcactaagcg tgaagtgttt
     1321 tccatctgcc atgatgccca cgatctggat cgacacgccg tagcggtttt taaaatagcg
     1381 tccgacggcg tgctcggagt ggaagagtcg ccgggcgaac tcctgattca caatggcaac
     1441 agccggcgtc ttcgcagtgt cggtaaagct tacatcgcgt cccgcgagca gtggtgttcc
     1501 agaggcggtc aaatatccag gtgaaacgcc gtagaaatag gtgtcaaatg ctttttttga
     1561 gggtctgaaa tcgatacttt gttgagagaa gacagcagaa gtgctttgct cgaaggatag
     1621 gggcgtggta ttcgcgtagc ctgctgcatc cactcccggg atctgcgaga ccctttccag
     1681 taactggcgc tggaaatgat cggcggctgc atcgctggta tagccagccc ggcccagatc
     1741 gaattgcgta cgcacagcat tcttcggatt gaaacccagg tccatggtca gtgctttgcc
     1801 caggccacgc agcgagacaa gagcggccgt gacggtgacg cagcagagcg cgatctgtgc
     1861 cgccagcagg acatcgcgaa gcgcccagcg gcggcctgca gaggataggc ttccgccgct
     1921 cttaattgcg tcatttggat cggtcttgaa tatctgccgc agtggaatca ccccgaacag
     1981 aacgccggcg agcaccgaga taaggaacgc catcaggatc aacgatggct gcggcagcac
     2041 ttggaatttg aaagggaatc tgctgggcgg atgccagccg gccaggccgg tgagggccat
     2101 ccacgccagg ccgcaggcgc acgcgccccc gaagatcgaa atcacaaggg cctcgacaag
     2161 gagctggcga aggatgcgcc agcggcttga tccaatggcc atgcggatgg caatctcgcg
     2221 cgtgcggtct gccgtacgcg ctgcgaatag gctgccaagg ttggcgcagg cggcgagcag
     2281 cacaatcccg gccagcccca tcaccccagc cagaaaaccg cgcgccggcc cgccgataaa
     2341 gtcgccgatc ataccaggac gagccagctt taaagccaac ccctcttcat ccttgggata
     2401 ctgccgggca atgcgggcag cgattgcgtt caatccagcc tgcgcctggg gcagagtgac
     2461 ctcgtccttg atgcgcacga tcgaaaagac attcttatgg ctccgcgact caagccagtt
     2521 gaccccgtcc agcgatgctt cattcgccat cggaacgaaa atgtcaggct gcgtaaattt
     2581 ttcggtcccg tagaagcctt ccggcgtgac gccgacgatc gtgtacggat gcttgtcaat
     2641 gcggaccatc gttcccacga ttttagggtc tgcgccaaaa tcactcttcc atgctggcca
     2701 ggaaagcacg gcagcctctg aggctccggg atgatcgtca tcggaacgct ccagcagacg
     2761 cccgaggaac ggcttgatgg cgacgacctc gaagtactgc ccgctcactt cgtatcccca
     2821 gacgtgccgg gtgacgccgt tggcttccaa accaaattcc tgcacctcaa caccggctac
     2881 agcagaaaac accgtattgt catcgcgcac atcgcgcaat tccggatagg caagaaaggg
     2941 gccaccgtgt ttgggctgaa gcgtcatcac ccggtctgca tgaggcacat caaggtgccg
     3001 caggactaat gcctgcagga cgccgaaaac gatgacattg gcggcaatgc caagcgccag
     3061 gataaggacc gcagtgaggg caaagccggg agaacggcgc atctggcgaa gggcaaatcg
     3121 caggtcctgc aacagggaat cgagaaaggc gcccgtccgc gaatcgcgca cttgctcctt
     3181 gacttgctcg actccgccaa gttcgatccg cgcggcgcgt tgcgcctcgt gcggcggcat
     3241 gcccgcgcga atgttttcct ctaccagcag ttcgaggtgc gaacgaacct cttggtcgag
     3301 gtctacatcc acacgccgag ataaaaaaag gtttctcagg aaacttcgga ctttcacaaa
     3361 gagcggcatg gtaaacacct caacaatcga aacgagcaga aaccaaattc tattcgtaac
     3421 gcaacgcaac cattgggtct accttcgtgg ccc
//
LOCUS       DASYVO010000379         9173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_264831, whole genome shotgun sequence.
ACCESSION   DASYVO010000379 DASYVO010000000
VERSION     DASYVO010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9173
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264831"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..459
                     /locus_tag="VGR03_12805"
     CDS             205..459
                     /locus_tag="VGR03_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995201.1"
                     /translation="MRCQFVLDKQTHNMLNGLAEARAGNRSFVVREAIRLYAAYESRL
                     DEIEKEPGFLRMMERTQADIRAGRTFTQAEVKKRLAKKKK"
     gene            complement(741..1493)
                     /locus_tag="VGR03_12810"
     CDS             complement(741..1493)
                     /locus_tag="VGR03_12810"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEV1995202.1"
                     /translation="MNSLEETAHRSWPLPRAAWIIRMMWEDLLFAHWPIPAAVLRPLV
                     PSSLELDTFDGSAWLGIVPFRMTGVRLRGLPPVPGTSAFPELNVRTYVSAQGKPGVWF
                     FSLDAASRIAVRAARAWFHLPYFDARMDSRGNGEEIAYSSVRIHRGAPTADFAARYRQ
                     TGEIQRAANGSLEHFLTERYCLYAGDTTNAGDPRLLRGEIHHSPWPLQPAEAEITVNT
                     MTAPLGFRFPDTPPLLHFARRLDVVAWPPRPM"
     gene            complement(1490..2848)
                     /locus_tag="VGR03_12815"
     CDS             complement(1490..2848)
                     /locus_tag="VGR03_12815"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEV1995203.1"
                     /translation="MNSTSASRNTANASGRGAGRQAAIALAGLIALETIFYYMHGLRD
                     LKLYVVETIALGLAAGVVYLLVIYALEHNAESRLTLWIVLAGALVFRVTLVPLTPTLS
                     DDIYRYQWEVRAQVAGLSPYVVAPSDPEAVPIRGPDWRRIPAPDVRTLYPPLTELFFR
                     AIGAVSTDPFAIKLPLVAVEWLVVGLLALLLRATGRRMSLLAIYAWNPLVVVESAASG
                     HNDSLAVAALLTALLLIIRRRPVLSTLALAAAVLSKLFPVVLAPLWLRLLGWPKKPRA
                     WAAIGVAAMFALMCSWPYRYDLPAIFAALRKFGDSFIDNASLFLALRWFSGSMEWATG
                     ISEGVVAGLALWAAARRLDPLRAAYLLFGTILLVSQNAFPWYFVWIIPLLCFFPNPAW
                     LLLTILQFLSYHVLIDYQAFDRFEFQPFFLWLTYGPSYAMLLWQVFKKGGTVISDQTR
                     AT"
     gene            2839..4470
                     /locus_tag="VGR03_12820"
     CDS             2839..4470
                     /locus_tag="VGR03_12820"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HEV1995204.1"
                     /translation="MSSLGSLAMWLMVTLGPFTGTWRRLTDRTFYGVYQANTFDMAIM
                     LPYFLILVVLASYGLHRYWLVYNFFKFSKNVPGPPPPVAEWPKVTIQLPIYNERYVIE
                     RLVEAVSRFDYAREKMQIQVLDDSTDETVEVARACVERHQSLGFDIHYHHRDNREGYK
                     AGALAEGMKSATGEFIAIFDADFQPHADFLRRTVPYFIDSKIGMIQTRWTYLNRHYSA
                     LTEVETILLDGHFVIEHGSRSRRGTFFNFNGTAGVWRRIAIEDAGGWEHDTLTEDTDL
                     SYRAQMRGWKFLYQPEVECPSELPVEMNAFKAQQARWAKGLIQTAKKILPRVFRSKQP
                     LHVKAEAFFHLTANISYPLMILLSTMLLPAMIVRFYQGWIQMLVIDLPLFLASTCSIS
                     SFYLTAQRHLRPKEWTRTFLYLPFVMAVGIGLAVRNSVAVIEALLGIQSEFARTPKYH
                     IEERGDSWLTKTYRNKSGWLPWIEIALGLYFAATVVYAVQNENYATVPFLLLFVGGYL
                     YTGIMSLGQGYLHRLRFGVSSAAEARAAATGAPSF"
     gene            4534..4935
                     /locus_tag="VGR03_12825"
     CDS             4534..4935
                     /locus_tag="VGR03_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995205.1"
                     /translation="MKNRERIARGLVVIGSLSLFAPAALRDFTGYPMLSRALGTVNPR
                     PFLEGSSRAFWHLLSWHWVVIGVLALVVSYGGTKLRKFAVLLCGLLAFMDAIGTIIAV
                     GLFLGNELLILAALAFLAAAVLFEDGHSLQA"
     gene            5172..6119
                     /locus_tag="VGR03_12830"
     CDS             5172..6119
                     /locus_tag="VGR03_12830"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEV1995206.1"
                     /translation="MITGGAGFLGSHLCDKLIEHGWDVLCIDNVVTGSEANVAHLEHN
                     EHFRILRHDVSRYIDVPGPVDYVLHFASPASPVDYLKLPIPTLKVGALGTHNALGLAL
                     AKKAKFFLASTSECYGDPLVSPQPETYWGNVNPIGPRGVYDEAKRYAEAMTMAYHRFH
                     GLDTHIVRIFNTYGPRMRLNDGRALPNFIYQALTGQPITVYGDGTQTRSFCYVSDLIE
                     GIYRLMLSDEHLPTNIGNPQEITIHEFAERVRLLIAKNSSIVFEPLPQDDPKRRCPDI
                     TKAKKLLGWEPKVGLDEGLRLTLEFFKRQLSKVPAGSGS"
     gene            6218..7048
                     /locus_tag="VGR03_12835"
     CDS             6218..7048
                     /locus_tag="VGR03_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995207.1"
                     /translation="MWPHFVNLLCRSWNALLNSLGTTTLSVVVFSVLVPLITNVVFAT
                     VHLFRQRKRSVGVSLGMQVKRALQENWLPTAISAGLLVAVWVILFSARVVSTTYDDHQ
                     SLVKGIGDLQGYARDKQKFDSELRQAHSDAEVWHNAYSGLLHGDIHPDRVLNSENSSK
                     LFEALKLVSKNPKNKEFVTVEMGGVQDREAISLRNQFWKIFNEARWIVKPRPKLGKEF
                     EACPTFTLGVTIWSENPQRGMYLVLLLKDAGVESNVNPCPMPQGVKGTIVWVGYKQWP
                     "
     gene            7444..7923
                     /locus_tag="VGR03_12840"
     CDS             7444..7923
                     /locus_tag="VGR03_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995208.1"
                     /translation="MTEEGPGLDLYDANGNLRASLGVSAEGPALRLWDAMTEQGLSLM
                     VDKDGPVVCLREVKGNTGVMVTAGKVGPAICLSDANGKHRLILDVSPFESGTPGLTMR
                     NANGMATVVVRTLEDGPSVGLFDPANEHGNTAVRLQVDSDGPSLRCLKDGKVLWSAT"
     gene            8383..8775
                     /locus_tag="VGR03_12845"
     CDS             8383..8775
                     /locus_tag="VGR03_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995209.1"
                     /translation="MRVRLALYAVVLCVCFAPILSAQDAAKVDPKHYKVVTENDQVRI
                     LKVHYGPHEKSVMHSHPATVAVFLTDSKGQFTFPDGKKEVFAPKAGEAQYAASATHLP
                     ENTSDNGMDVIVVELKGKAAKPAKATKK"
     gene            complement(8886..>9173)
                     /locus_tag="VGR03_12850"
     CDS             complement(8886..>9173)
                     /locus_tag="VGR03_12850"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HEV1995210.1"
                     /translation="RKLLAEGASLRAYDPEAMEKTRVIFPQVHYAEPYEVADKADALL
                     IVTEWEQFKKLDWTRVRDSMLRPLVFDGRNLLDPAKMKELGFEYQSIGRPI"
BASE COUNT         1981 a         2692 c         2698 g         1802 t
ORIGIN      
        1 cccgtcgacc gctcgaagaa tccgctgccc cccgcccgtc ccgccacgac tctggtcaca
       61 ctcaatactt agcccgggct tctgagctct tgatcgcagg cccatttgtc cttccgtgcg
      121 agaacagaat tgagtccaca acattcatga tctgtgttga ctaagaggct tcttcgccgt
      181 attcttgccc cggggagaaa attcatgcgc tgccagtttg tcctcgacaa acagactcat
      241 aacatgctga acgggcttgc agaggcccgc gccggtaacc gaagctttgt tgtgcgcgaa
      301 gcgatccgac tgtacgccgc ctacgagtcc cgtctggatg aaatagaaaa agagcccgga
      361 ttccttcgaa tgatggagcg cacgcaagcc gacatccgcg cgggccggac gttcacacag
      421 gcggaggtca aaaagcgact ggccaagaaa aagaagtgaa cgcgcgagtc cattggccgc
      481 acttgctgtt tgaagtcttg tctgctctgc ccccgaaaga gcaggacttg atcctggaaa
      541 gaactcgtta tcttgaaatg ttcccgcgga tgtatcccgt gcgagtccgc ggccgctttc
      601 gcagacaccg ctggtttctt gccggagatt ggctggtttt ttatcaggta accggcaatg
      661 acgttttcgt tcgtggcctt tggcctgcgc gaatgccgta gttttcacct cactcgcaac
      721 agcggatcag aagacgggcc ttacattggc ctcggtggcc aggcgacgac gtcgaggcgg
      781 cgggcgaagt ggagcagcgg cggcgtgtca gggaaacgaa agccgagcgg agcggtcatg
      841 gtgttaaccg tgatctcggc ctctgcgggt tgaagtggcc agggcgagtg atgaatctcg
      901 ccgcgcaaga ggcgtggatc acctgcgttt gtggtgtctc ctgcgtacag gcagtagcgc
      961 tcggtcagaa agtgttcgag tgaaccgttt gcggcgcgtt gaatctctcc tgtctgccgg
     1021 tagcgcgcag cgaaatctgc cgtcggcgcg ccgcgatgaa ttcgcacgct ggaatacgcg
     1081 atctcttctc cgttcccccg cgaatccatc cgcgcatcga aataaggcag atggaaccac
     1141 gctcgcgccg cgcgcacggc gatacggctg gcggcgtcga ggctgaaaaa ccagacgccg
     1201 ggctttcctt gcgcggacac ataggtccga acattgagct cgggaaacgc cgaggtgccc
     1261 gggactggag gaaggccgcg cagccgcacg ccggtcatgc ggaacggcac gatgcccagc
     1321 cacgcgctgc cgtcaaacgt atccaattcg agcgacgatg gcacaagagg ccgcagcacc
     1381 gctgcaggga ttggccaatg tgcgaagagc aagtcttccc acatcatgcg tatgatccac
     1441 gcggcgcgcg gcagcggcca tgatcgatgc gcggtttcct cgagagagtt catgttgctc
     1501 ttgtttggtc ggaaattacc gtgccgccct tcttgaaaac ctgccacagc agcatggcgt
     1561 acgacggccc atacgtgagc cacagaaaaa acggctgaaa ctcgaaccgg tcgaacgcct
     1621 ggtagtcgat gaggacgtgg taggagagaa actgcaagat agtgagcagc agccaggccg
     1681 gattcgggaa aaaacagagc agcggaataa tccacacgaa gtaccaggga aatgcatttt
     1741 gtgaaaccag caggatcgtg ccgaacaaca aatacgccgc gcgcagcgga tcgagccttc
     1801 gcgccgccgc ccacagcgcc aatccagcga ccactccctc actgattccc gtcgcccatt
     1861 ccatggagcc gctgaaccaa cgcagcgcca gaaacagact tgcattgtcg atgaacgagt
     1921 caccgaactt gcgcaaagcc gcaaaaatcg ccggcaaatc gtatcgatag ggccacgaac
     1981 acatcaaggc gaacatcgct gcgacaccga tggccgccca ggcgcgcggt tttttcggcc
     2041 acccaaggag acgcagccac agcggcgcca gcaccaccgg gaacagtttc gaaaggacag
     2101 ccgccgccag cgccagcgtt gacagcaccg gcctcctccg tataatcagc aggagggcgg
     2161 ttagaagggc agctacggcc agtgaatcgt tgtggccgct cgcggcagac tccaccacca
     2221 ccagtggatt ccaggcgtag atggcgagca gggacattcg tctccccgtg gcgcgaagaa
     2281 gcaacgcgag cagcccgacc accagccatt cgacggctac cagcgggagc ttgatggcga
     2341 acggatcggt tgataccgcg ccgatcgcgc gaaaaaaaag ctcggtcagc ggcggataaa
     2401 gtgtgcggac gtccggggca ggaatccggc gccagtcggg gccgcggatc ggcacggctt
     2461 ccgggtcgga cggcgcaacg acgtagggac tgagtcccgc gacctgcgcg cgcacctccc
     2521 actgataccg gtaaatgtcg tcggagagcg tcggcgtgag cggaacaagc gtcactcgaa
     2581 ataccagcgc gccggccagc acgatccaca gcgtaagccg cgattccgcg ttgtgctcca
     2641 gcgcgtaaat gacaagcagg tagacgacac ccgcggccag acccagcgcg atcgtctcga
     2701 ccacgtaaag tttgaggtcg cgcaggccat gcatgtagta aaagatcgtt tcgagagcga
     2761 tcagtccggc gagggcgatg gcggcttgcc ggccggcgcc tcggccgctc gcgttcgccg
     2821 tattcctgga ggcagaagtt gagttcactc ggcagcctgg cgatgtggct gatggtcacg
     2881 ctgggcccat tcacgggcac ctggcggcgg ctgacggacc ggacttttta cggcgtctat
     2941 caggccaaca ccttcgacat ggccatcatg ttgccgtatt ttctgattct ggtagtcctg
     3001 gcgagttacg gtctgcatcg ctactggctg gtttacaatt ttttcaaatt cagcaaaaac
     3061 gttcctggac ctccgccgcc tgtcgcggag tggcccaagg ttaccataca gcttcccatc
     3121 tacaacgagc gctacgtaat cgagcggctc gtcgaagccg tctcgcgctt cgactacgcg
     3181 cgcgaaaaaa tgcagatcca ggtgctcgat gattcgacgg acgagacggt ggaggtcgcg
     3241 cgcgcctgcg tcgagcgcca ccagtcgctg ggcttcgaca ttcactacca ccatcgcgac
     3301 aatcgcgagg gctacaaggc cggcgcgctc gctgagggca tgaagagcgc caccggcgaa
     3361 ttcatcgcca ttttcgacgc cgacttccag ccgcatgcgg atttcctgcg tcgcaccgtg
     3421 ccctacttca tcgactcgaa gatcggaatg atccagacgc gctggacgta cctgaaccgg
     3481 cactactccg cgctcaccga agtggaaacg attctgctcg acggccactt cgtcatcgag
     3541 cacggctcgc gctcgcgccg cggcaccttc ttcaatttca acggcaccgc cggcgtctgg
     3601 cggcgcatcg ccatcgagga cgccggcggc tgggaacacg acacgctcac cgaagacacc
     3661 gacctttcct accgcgcgca aatgcgcggc tggaaattcc tgtatcagcc ggaagtcgaa
     3721 tgcccgtcgg agctgccggt ggagatgaac gccttcaagg cgcagcaggc gcgctgggcc
     3781 aaggggctga tccagaccgc aaaaaaaatc ctgccgcgcg tcttccgctc caagcagccg
     3841 ctgcacgtca aggccgaagc cttcttccat ctcaccgcca acatcagcta cccgctgatg
     3901 attctgcttt cgaccatgct gctgccggcg atgatcgtgc gtttctatca gggctggatt
     3961 cagatgctgg tgatcgacct gccgctgttc ctcgcgtcga cgtgctccat ctccagcttc
     4021 tacctgacgg cgcagcgcca tctgcgcccc aaggaatgga cgcgcacgtt tctctatctg
     4081 ccgttcgtga tggccgtggg gatcggcctc gccgtgcgca actccgtcgc ggtgatcgaa
     4141 gcgctgctgg ggattcagtc cgaattcgcg cgcacgccga agtaccacat cgaggagcgc
     4201 ggcgactcct ggctgacgaa aacgtatcgc aacaagtccg gctggctgcc gtggattgag
     4261 atcgcgctcg gtctctactt cgccgccacc gtcgtctacg ccgttcagaa cgagaactat
     4321 gccaccgtcc cgtttcttct gctttttgtc ggcggctacc tttacaccgg catcatgtcg
     4381 ctcgggcagg gatatttgca tcgcctgcgc ttcggagttt ccagcgccgc agaggcgcgc
     4441 gccgcggcga ccggcgcccc ttccttttaa gcgaatggat tgccggcccg cagtttcaaa
     4501 gtgtgctggg cagaaccaga cggggagttg accatgaaaa atcgtgaacg aattgcgcgg
     4561 ggactcgtgg tcatcggcag cctcagcctt ttcgcaccgg cagcccttcg tgatttcact
     4621 ggttatccca tgctctcacg ggcgctcggt accgtgaatc caagaccgtt tttggagggc
     4681 agctccagag ccttttggca tctgctgtca tggcactggg tcgtgattgg ggtgttggcc
     4741 ctggtggtct cctatggagg aacgaaactg cggaaatttg ccgtcttgct gtgtggattg
     4801 ctcgccttca tggacgccat cggcaccatc atcgccgtcg gattgttttt gggtaacgaa
     4861 ctgctgatcc tcgcagcgct ggccttcctc gctgctgcgg ttcttttcga ggatggccac
     4921 agcctgcaag cctgagcgct gcccgctacg ggagcaaatg ggactctagc tcttccctcg
     4981 gtcattccga gtccgccgcg gcggatgtct gcatcccgat tccatcggga cgaagaatca
     5041 ctcttctcat atctcctttc cgtcatcggc cgcgcggact acaataccgc cctggcggag
     5101 cgaagggccg ttccatctct ccgcgcaaca tccggagcag gcgaaatgtt gaggcgaaaa
     5161 atttgcgcgc agtgataacc ggcggagcgg gttttctcgg gtcgcacctg tgtgacaagt
     5221 tgattgagca cggctgggac gtgctgtgca tcgacaacgt ggtcaccggg agcgaggcca
     5281 acgtcgccca cctggagcac aacgagcatt tccgcattct ccgccacgac gtcagccgct
     5341 acatcgacgt gcccggaccg gtggactacg tcctgcactt cgcctcgccc gccagcccgg
     5401 tggattacct gaagctgccc atccccacgc tgaaggtcgg cgcgctgggc acgcacaacg
     5461 ctctgggact ggcgctggcg aaaaaggcga aattttttct cgcctcgacg tcagagtgtt
     5521 acggcgatcc gctggtgtcg ccgcagccgg agacctactg gggcaacgtg aatcccatcg
     5581 gaccgcgcgg cgtctacgat gaggccaagc gctacgccga agccatgacc atggcctacc
     5641 accgctttca cggcctggac acgcacatcg tgcgcatctt caatacctat ggcccgcgca
     5701 tgaggctcaa cgacggccgc gcgctgccga attttattta tcaggcgctc accggccagc
     5761 ccatcaccgt ctacggcgac ggcacgcaga cgcgcagctt ctgttacgtc tccgacctga
     5821 tcgagggcat ctatcgcctg atgctttccg acgagcactt gcccaccaac atcggcaacc
     5881 cgcaagagat caccatccac gaatttgcgg agcgagttcg tctgctgata gcaaaaaact
     5941 cctcaatcgt ttttgagccg ctgccgcagg acgatcccaa gcgccgctgc ccggacatca
     6001 ccaaagcgaa aaagcttctc gggtgggagc cgaaggtggg gctcgatgag gggttgcgcc
     6061 tgacgctgga gtttttcaaa cggcaactgt ctaaagtgcc agcggggtcg ggaagttaaa
     6121 ttaagttttc gttgatcctg ggtaccctcc cgcccccgat tttgcaaggg caacaacata
     6181 ctagatgcat aaagcgtcta ggtatacgat gcgctttatg tggccccact tcgtcaatct
     6241 gctgtgtagg tcatggaacg cactactcaa ttcactcggt acgacaacac tcagtgttgt
     6301 ggtattcagc gtgctcgttc ctcttatcac gaatgtcgtt tttgcaacgg tgcatctgtt
     6361 ccgacagcgc aagcgctcgg tcggcgtgag tttgggcatg caggtcaaac gagcgcttca
     6421 ggagaactgg ttaccaaccg cgattagcgc ggggctactt gttgcggtat gggtgatttt
     6481 gttcagcgca agagtggtca gcacgacgta cgatgaccac caatcattgg tgaaggggat
     6541 tggtgatctt caaggatacg ccagagacaa acagaaattc gacagcgaat tgaggcaagc
     6601 gcattccgat gctgaggttt ggcataatgc atattcagga ctgttgcatg gcgacatcca
     6661 tccagacaga gtgctaaata gcgaaaatag cagcaagctt tttgaagccc ttaaacttgt
     6721 atctaagaat ccaaaaaata aggaatttgt aaccgtggag atgggcggcg ttcaagatag
     6781 agaagccatt agtttaagga atcagttttg gaaaatcttt aacgaagcgc gttggatcgt
     6841 caaaccacgc ccgaaactcg gcaaagagtt tgaggcgtgc ccgactttta ctcttggggt
     6901 gacgatatgg agcgagaacc cgcaaagggg aatgtattta gtattgctgc tgaaggacgc
     6961 cggtgtggaa agcaatgtta atccgtgccc gatgccgcaa ggcgtcaagg gtacgatagt
     7021 atgggttggg tacaagcaat ggccatgaag cgcgaatatc agcaggggcc gaaagttaga
     7081 gaaaacttcg agcaagttat ggtgcctctt tttcaggaat cgaagctaga agtaaaaaag
     7141 gaaacccaaa aagaaaggcg ggtggcacgg acgtgaagta agagacgaat ctgagtgccc
     7201 gccctctgtc gcgtgaactt gatgacggta actgatagac tccctgctat tagttgcaat
     7261 tacggaccat cacccaatcc tggagattac gccatgcaag actcgacttt ggaccttcca
     7321 tccctcgctg cccgaattga gaaactggaa aaacagatgg accaagctca gccaagccgg
     7381 acacttgtga cggagaaact tgtccttacg gatgccagtg ggaaaacgag agcgtctctg
     7441 cgcatgactg aagaggggcc ggggctcgac ctttacgacg cgaatgggaa cttgcgtgcg
     7501 tccttgggtg taagtgcaga agggccggct ctccgcctct gggatgcaat gacagaacaa
     7561 ggtttgtcac tgatggtaga taaggatggc ccggtggtat gcctccgcga agtgaaaggg
     7621 aataccggtg ttatggtgac ggcaggtaag gttggcccag caatctgcct gtccgacgct
     7681 aacggaaagc accgtctgat cctggacgta agcccgtttg agtccggcac gccgggtctg
     7741 accatgcgca acgcgaacgg aatggccaca gtggttgtga ggacactgga ggatggtcca
     7801 agcgtaggct tgttcgaccc ggcgaacgag cacggaaaca cagccgtgcg gctccaggta
     7861 gacagcgacg gaccgagtct gcggtgcctt aaagacggta aagtcctgtg gtccgcgacc
     7921 tagcgtatct gccttcaccg ttaccgccct cgcttgttgg gcgggtggcc cgcacgtcgt
     7981 tgtttacgtg tggggaactt cttcagcctt ctctatgcac tcctctatcc cgcaaggtcc
     8041 agcaccccgc gggtaagtac gaccgtgggc ttaccccctc tccggtctgc acggtgaatc
     8101 gcaggattgc gatcctctcg tggatgcctg ccgtcaacag aaaagaagaa aatcatcggg
     8161 cccacgcgtt gggaggatcc taccggccgc gcgccgcccg actttgcccc cagccgggtg
     8221 gcgatctcca accgccgctt ttcgctcaga cattcgagcc gcgtcaggag gatacgatgt
     8281 gagcaggctc ttccaaaatg aaagcctgat tgacagcagc aaccctgccg gaatagagtt
     8341 cggtaccttt gcagcaaatc ccacaagccc aaggagggca acatgcgagt gcgactagcg
     8401 ctttatgcgg tggtgttgtg cgtctgtttc gccccaatcc tctcggccca agacgcggcc
     8461 aaggttgatc ccaagcatta caaggtagtg accgaaaacg accaggtacg gattctgaag
     8521 gtgcactacg gcccgcacga gaagtcggtc atgcacagcc atccagccac ggttgcggtg
     8581 ttcctcaccg actccaaggg gcagttcacc tttcccgacg ggaaaaagga agtctttgct
     8641 cccaaggcag gcgaggcgca atacgccgcg agcgccaccc acttgccgga gaacaccagc
     8701 gacaatggga tggatgtgat cgtagtcgag ctgaaaggca aggcggccaa gccagcgaaa
     8761 gcgacgaaaa agtaatttcc ggcgatgaag cgggtggcgc ggacgtgaag taagagacga
     8821 gcctgggtgc ccgcacgtcg gtattacgcg tgtggctttt tcgaaggaag ctggcgttgc
     8881 gcgcgctaga ttggccggcc gatggactga tactcgaagc ccaattcctt catcttcgcc
     8941 gggtcgagca gattgcggcc gtcgaacacc aggggccgca gcatggaatc gcgcacacgc
     9001 gtccagtcga gcttcttgaa ttgttcccac tccgtgacga tcagcagcgc atcggccttg
     9061 tccgccactt cgtagggttc ggcgtaatgc acctgcggaa aaatcactcg cgttttttcc
     9121 atggcctcgg gatcgtaagc gcgcagcgaa gcgccttccg ccagcagctt gcg
//
LOCUS       DASYVO010000380         4902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_276922, whole genome shotgun sequence.
ACCESSION   DASYVO010000380 DASYVO010000000
VERSION     DASYVO010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276922"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="VGR03_12855"
     CDS             <1..1347
                     /locus_tag="VGR03_12855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1995211.1"
                     /translation="PVQNAASSQRSPTNGLLLGLVITLAAVLAYAFYITVQLTGLRKL
                     QSEMVDRNRKDSLQLLRVQNDLNSIALAMRDMLDTTEPYPLTAWTAQFERIHQDLDAG
                     LKLEESVAGANRTPEQRMFLSQSVAQFWDAVNRMFVLAQNGKDEEAREQIRLSLQARQ
                     QALSTAVSRLLVENNEGEEQAAARIAQIYDGVQRQLYIFLAATLIAIVLTSLYLIRSN
                     RQIFARLAELSEQRSDLAQKLISTQESTLRHISRELHDEFGQVLTAIGSMLGRAGKHA
                     PEESPLREDLKEVQEIAQSTLNNIRTLSQALHPVLLEEAGLESTLDWYIPTVGRQTGL
                     ALHYEKTGQPFPVEMSAGVHIYRVLQEALNNVSRHSGASDAWIRLRFSPDSLELEVED
                     HGTGFVPEKMQRGIGLVAMRERAELIGGTLAISPRPQGGTKVRLQIPRVKVDANGA"
     gene            1337..1966
                     /locus_tag="VGR03_12860"
     CDS             1337..1966
                     /locus_tag="VGR03_12860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1995212.1"
                     /translation="MAPKITVLLADDHALVRRGFRRMLEDDPDISVVGEASDGKEAVR
                     LACELRPRVTVMDCAMPELSGIEATRKILEKFPEAGILMLSMHSEETLVSQALGAGAR
                     GYVLKNAMDLDLVSAVKSIAAGMTVLDPQVSRQDNLKGERGGGLTPRELEVLKHIVGG
                     KSNKEIAVDLHLSVNTVAVHRANIMDTLGIHKTAELVVYAIRNGLVNLP"
     gene            1963..3660
                     /locus_tag="VGR03_12865"
     CDS             1963..3660
                     /locus_tag="VGR03_12865"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEV1995213.1"
                     /translation="MNRRDFLFTALGAGLTTTAAPAWMFANPASSLGFRLTDVTNASG
                     IQFRHNSGAYGGKLLPETLGSGCAFLDYDGDGWQDILLVNGMDWPEHKRQRSTLRLYR
                     NNRNGTFTDVTRSAGLNVEMYGMGVAVGDYNNDGFPDIFITCVGQSRLFRNTGKGTFV
                     DATRSSGLFGRQGFSTSALWFDYDRDGLLDLFVCNYVRWSPEHDVFCSLDGSHKSYCT
                     PEAYRGDTCWLFHNRGNGTFEDVTATSGIFDSSSKSLGVAMFDYDQDGWPDLLVANDT
                     QPNKLYRNLRNGKFKDVAVEAGLAFSTEGKARAGMGVDAGDFDGSGKPGVAITNFDNE
                     MIGLYRASAPGVFDDVSIPSGIGAASKNTLGFGCMFLDVDLDGALDLAVANGHIDETV
                     RNIRGNTGYAQPPQLFLNRGGGKFRDVAEEAGGSFETPKVGRGLAYGDFDGDGDLDIL
                     MTTNNGPAYLYRNDQTANPRSIRFHLKGTKSNRDAIGSSVRIFHEGQTQSRMIRGGSS
                     YLSQSEMPVTFGLGLREKIDRVVIDWPSGRTEEYKDLHAGKTYNCMETKGIDAQPGF"
     gene            3841..>4902
                     /locus_tag="VGR03_12870"
     CDS             3841..>4902
                     /locus_tag="VGR03_12870"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEV1995214.1"
                     /translation="MMQIDFVKRWLSRSLVALTVSAVYLYGYPSATISYFVADLFHIA
                     VGIVLTILLFFYVVRLLRRETLLARLGWISLAAGALLGIVLIKIGTPLRLKPWLYAHI
                     ALCVLGTLFLTTSWLFSKGWLGDGVIRRALGFAALTLLTAGMAAGTWWTREVAWKNAN
                     RISNPLMPPETMDSEGDGAQGKFFPSSAQTKHGGNIPSKYFMKSDACQRCHADIYKQW
                     DSSMHHFSSFNNQWYRKSVEYMQDVAGVRSSKWCAGCHDPALLYSGLFDTPIKQIVDR
                     PEARAGLGCMMCHSIVQVKSTMGQGDFFLEYPKLHELAASENPLVRSLHDFVVRLNPE
                     PHRRTFLKPFMRADTAEFRS"
BASE COUNT         1064 a         1373 c         1480 g          985 t
ORIGIN      
        1 cccgtgcaga atgccgcatc ctcgcaacgc tcacccacga acggactgct gctggggctg
       61 gtgatcacgc tggctgccgt gctggcctac gccttctaca ttaccgttca gcttaccggc
      121 ctgcgaaaac ttcagagcga gatggtggat cgaaaccgga aagactcgct gcaactgttg
      181 cgcgtccaga acgatctcaa ttccatcgcg ctggcaatga gagacatgct ggataccaca
      241 gaaccctatc cgctgacggc atggacagcg cagttcgaac gcattcacca ggacctggat
      301 gccggtttga agctggaaga gagcgtggcg ggggccaacc gcactccgga gcagcggatg
      361 tttctctctc aatcggtggc gcagttctgg gacgccgtga accggatgtt cgtgctggcg
      421 cagaacggca aggatgagga ggcgcgagaa caaattcgat tatcgctgca ggcgcggcag
      481 caagccctca gcacggcggt ttcacggctg ctggtggaga acaacgaagg tgaggaacaa
      541 gccgccgcac gcatcgctca gatttacgat ggtgtgcagc ggcagctcta tattttcctg
      601 gcggcaacgc tcatcgcgat tgttctgacg agcttgtatc tgattcgcag taaccgccag
      661 atttttgcgc gcctggcaga gctctccgag cagcgcagcg atttggcgca gaaactgatc
      721 tcgacacagg aatcgacact gcggcacatt tcgcgcgaac tccacgatga gtttgggcag
      781 gtcctgacgg cgatcggatc gatgctgggc cgggcgggca agcacgcccc ggaagaatca
      841 ccccttcgcg aggatctgaa ggaggtgcag gagatcgcgc aatcgacact aaacaatatc
      901 aggacactct ctcaggcact tcatccggtt ctactcgaag aggccgggct agaaagcact
      961 cttgactggt atattcccac ggtggggcgg caaaccggcc tggcgctaca ttatgagaag
     1021 acgggacagc cgtttcccgt ggagatgagc gcaggcgtgc atatctaccg ggtgctgcaa
     1081 gaagcgctga ataacgtgag ccggcattcc ggggcgagtg acgcctggat ccggctgaga
     1141 ttctctccgg attcgctgga gctcgaagtg gaagatcacg gcacgggttt cgtaccagaa
     1201 aagatgcaac gcgggatcgg gttggtggcc atgcgggaac gcgccgaatt gattggcggc
     1261 acgctggcaa tctctccgcg gccgcaaggc gggaccaagg tccgtctcca gattcccagg
     1321 gtaaaagtgg atgccaatgg cgcctaaaat tacggtactg ctcgcagacg atcacgcgct
     1381 ggtacgccgc ggatttcgcc gcatgttgga agatgacccg gatatttccg tcgttggaga
     1441 agccagcgac ggcaaggaag ccgtgcgcct agcctgcgag ctgcggccca gagttacggt
     1501 gatggactgc gcgatgccgg aattgagcgg catcgaagca acgcgaaaaa tcctggagaa
     1561 atttccagaa gcgggaatcc tgatgctgag catgcattcc gaggaaacgc tggtaagcca
     1621 ggcgctcgga gcgggcgcgc gcggctacgt gctgaaaaat gccatggacc tcgacctggt
     1681 cagcgcggta aagagcattg cggcgggcat gacggtgctc gacccgcaag tgtcgcgcca
     1741 ggacaatctc aaaggagaac gcggcggagg attgacgccg cgcgagctgg aagtcctgaa
     1801 acacatcgtg ggcggcaagt cgaacaagga aatcgcagtg gatttgcatc ttagcgtcaa
     1861 taccgttgcg gtgcaccgcg ccaatatcat ggacacgctg gggattcaca aaacggcgga
     1921 actggtggtc tacgcgattc gaaatggcct ggtgaacttg ccgtgaaccg gcgcgacttc
     1981 ctattcacag cattgggcgc aggactaacc acgacggcgg caccagcctg gatgttcgcg
     2041 aatcctgctt cttccctggg attccgcctg accgatgtta cgaacgcttc ggggatccag
     2101 ttccggcata acagcggcgc ttatggcggc aagttgctcc cagagacgct gggatcgggc
     2161 tgcgcgttcc tcgattatga cggtgatggc tggcaagaca tccttctcgt gaacggcatg
     2221 gactggcccg agcacaagcg ccagcgatcc acgctgcggc tctaccgcaa caaccgcaac
     2281 ggcaccttta ccgacgtgac gcgaagcgca ggcctcaacg tcgagatgta cggaatggga
     2341 gtagccgtcg gcgattacaa caacgatggt tttcccgaca ttttcatcac ctgcgtgggg
     2401 caaagccggc tttttcgcaa caccggtaaa ggaaccttcg tggatgccac gcgttcgagc
     2461 ggcctcttcg ggcgtcaggg cttcagcact tccgcactgt ggtttgatta tgaccgggat
     2521 ggattgctgg atcttttcgt gtgcaactac gtgcgctggt cccccgagca cgatgtcttc
     2581 tgcagcctcg atggttcgca caagtcgtat tgcacgccgg aagcgtatcg cggggatacc
     2641 tgctggctct tccacaaccg cggcaacgga actttcgaag acgtaacggc taccagcggc
     2701 atctttgaca gtagctcaaa atctctcgga gtggcgatgt tcgactacga ccaggacggc
     2761 tggccggact tgttggtagc caacgacacg caacccaaca agttgtaccg caatttgagg
     2821 aacggcaaat tcaaggatgt cgcggtggag gccggcctgg cgttcagcac agagggaaaa
     2881 gcgcgcgccg gcatgggcgt cgacgccgga gatttcgatg gttcgggtaa gcccggtgtg
     2941 gccatcacca acttcgacaa cgaaatgatc ggtctgtatc gcgccagcgc tccgggagtt
     3001 ttcgacgacg tgtccattcc atccggcatc ggggcggcat ccaagaacac tctcggattt
     3061 ggctgcatgt tcctcgatgt ggacctggat ggcgctctgg acctggcggt cgccaatggc
     3121 cacatcgatg aaactgtccg gaatatccgc ggcaacaccg gatacgcgca accgccgcaa
     3181 ctcttcctga accggggcgg tggaaagttt cgtgatgtgg cggaagaggc cggaggaagc
     3241 tttgagactc caaaggttgg acgggggcta gcctacggag atttcgacgg cgacggtgac
     3301 ctggacatcc tgatgaccac caacaatggg ccagcgtact tataccggaa cgatcaaacg
     3361 gcgaatcctc ggagcattcg atttcactta aagggtacga agtcgaatcg cgatgcaatc
     3421 ggctcgtcgg tccgcatttt tcacgagggg cagacgcaat cgaggatgat ccgaggcggg
     3481 tcaagctatc tttcccaatc ggaaatgccg gtaacgtttg gcctcggctt gcgcgaaaaa
     3541 atcgatcgtg tggtgatcga ctggcccagc gggcgcacgg aggagtataa agacctgcat
     3601 gccgggaaaa catacaactg catggaaaca aaggggatag atgcccaacc cggcttctaa
     3661 tgcttttaca ttattgccag tccccgcgcc aatccatata actgtgacca caagcaacat
     3721 cgcctccagc ctcgccaatt cttggctcgc ttcggggcac cccaaactga atcgcggccg
     3781 gatgttacct agacatccgg ccgctgtttt gataaccaca aaccgcgcac gcaggtggag
     3841 atgatgcaaa ttgattttgt gaagcggtgg ttatcacgga gcctggttgc gctgacagtc
     3901 agcgcggttt acctgtacgg atatccctca gccacgatct cttactttgt tgcggatttg
     3961 tttcatatcg cggttggaat cgtgctaacg attcttctct ttttttatgt cgtccgcttg
     4021 ctccgccgcg aaacactgct ggcccgtctg ggttggatct cgctggctgc gggagcgctt
     4081 ctcggaatag tgttgatcaa aatcggtacg ccgcttcggc tgaagccgtg gctgtatgcg
     4141 catattgcgc tgtgcgttct tggcacgctt tttctgacga cctcttggct cttttcaaag
     4201 gggtggctgg gtgacggcgt catccgccgc gcattgggat ttgccgccct gacgctactg
     4261 acggcgggca tggcggcggg aacgtggtgg acgcgcgagg tcgcctggaa gaacgcgaac
     4321 cgcatttcca atccactgat gcctccggaa acaatggaca gcgaaggtga cggcgctcag
     4381 ggaaaattct tccccagctc cgcgcagacc aaacacggcg gaaacattcc ctccaagtac
     4441 ttcatgaaat cggacgcttg ccagcgctgc catgcggata tttacaagca gtgggacagc
     4501 tcgatgcacc atttctcttc gttcaacaac cagtggtacc gcaagagcgt cgagtacatg
     4561 caggatgtcg ccggcgtgcg ttcctcgaag tggtgcgccg gctgccacga tcccgcgctg
     4621 ctctacagcg gcttgttcga tacgcccatc aagcaaatcg ttgaccgccc ggaagcccgc
     4681 gccggcctcg gttgtatgat gtgccacagc attgtgcaag tgaaaagcac catggggcag
     4741 ggcgactttt tcctggaata cccgaagctt catgagctgg cggccagcga gaatcctctg
     4801 gtacgctcgt tgcacgactt cgtggttcgc ttgaacccgg agccgcaccg ccgcaccttc
     4861 ctgaagccat tcatgcgcgc ggacaccgcg gagtttagat cg
//
LOCUS       DASYVO010000381         6852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_249231, whole genome shotgun sequence.
ACCESSION   DASYVO010000381 DASYVO010000000
VERSION     DASYVO010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6852
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249231"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..2773
                     /locus_tag="VGR03_12875"
     CDS             293..2773
                     /locus_tag="VGR03_12875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1995215.1"
                     /translation="MRRIHWLTDFLDDARYAFRSLRRTSGLTAFVVITLALGIGMTST
                     PFSMLDALIFRPYPVPHPSSVVSLVSTSHDNSFDNFSYREYLDIRDNTRSYDGVIANG
                     QLGAVGFSAEPEATPRVRGGMMVSGNYFQVLGVEPQLGRGFREDEDEVPGRDAVVVLG
                     PDFWKHEFASDPSVVGRIIRLNGTDFTVIGVAPETFPGMQIFLRPDFYMPLAMARVFS
                     TNPQKNFLVDRDDRELSVKARLKPGTTLRQARNELAVLARDFEREYPKVNRDRGAAVR
                     TQFEMRTQDDDVNWKFGVIFTILALAVLLVACTNVAGLLLSRARTRTREIAVRLAMGA
                     GRFRLIRLLLTESLVLACLGGLGGIAVGYGAIKFLSTFRIPTELPVTIPFRMDTRVLL
                     ASLALSVLSALLCGLAPALQSTRVDLVNGLKSADVDVPGRKRLWGRNVLVVAQVSTSL
                     MLLTASFLMARSFQHSLLDGIGFAKDHLLMARFDPRLVQYNAAQTRQFYKLLAERVRV
                     APGVQSAALTQNPPLGLNNFDRVAFVPDGFQMPRDRENFTSTMDTVDEGYFETLGIPI
                     MRGRGFLASDTADAPRVAVVNEQFAKHYWPGADAVGKHIRLDSGVGTPVEIVGVAQTI
                     KYRETTERPTDFVYMPLAQHPVARMVLLVRSSGDPLQLVKPVKNIVRTLDPNLPMLET
                     RTYEDLYRYHAVEGPRVAIELVGTLGAVGLLLAIAGLYGLVAYNVSRRTREIGIRMAL
                     GAGPSDVLRLVMGKGLMLVGIGTVIGLAMGFAVEQLMNSMLFNAGGVDIVAYVIVVPS
                     MFLVTMLAAYVPARRASRIAPIQALRYE"
     gene            3176..3472
                     /locus_tag="VGR03_12880"
     CDS             3176..3472
                     /locus_tag="VGR03_12880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1995216.1"
                     /translation="MDLLILKSLIAGEMHGLGISRRIQQITGGTFVVKPGSLFPALHR
                     MDEEGWISSFWGDSENNRRAKYYRLTKPGRNQLEVETKRWGRISWAIAQALEAS"
     gene            3486..6137
                     /locus_tag="VGR03_12885"
     CDS             3486..6137
                     /locus_tag="VGR03_12885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1995217.1"
                     /translation="MPLLVKARSFLRNLFLSRRVEADLDQEVHSHLEMLIEENIRAGM
                     PPKEAQRAARIELGGVEQVKEQVRDARTGAFFDSLLQDLRFALRQMRRSPGFALTAVF
                     TLALGIAANVIVFGVLQALVLRTLDVPHGDRVMTLQPKDGGPFLSYPELRDVRDDNTV
                     FSAVAALEINEFGLEANGVTRPVWGYEVSGQYFEVVGIKPFLGRLLQRADDDHPGASE
                     AAVLSWPAWKSDFGADPNIVGTTVRIDKHPYTIVGVTPEAFYGTEKFGLVDIFVPMAN
                     QASLDGVDWLESRSHKNLFSIVRIKDGVTMPQVQAELNTIVARMARQYPKEEEGLAFK
                     LARPGLVGDFIGGPMRGFLAGVMGLAGIVLLAACANLGSLFAARTADRTREIAIRMAI
                     GASRWRILRQVLVEAFVISIFGGACACGLSWIALTGLASWHPPSRFPFKFHVLPQPSL
                     ILIAFLISVFAGVLFGMMPLRQIFKTDPNDAIKSGGSQSSAGRRWALRDVLLAAQIAL
                     CCVTVTAAFVSLRGLSKALTMDLGFNTKNAVRTTLNLSQAGYTTDSADPFQRQLLERV
                     SQLPGVEAAGYANTTPLDFDASITAVFSQQTTDFRPSNKAFEAYWYDVSPGYLTAAGT
                     PLLAGRDVSFTDTAKTPAVAIVNQEFARRLFHSEDAVGRYYKNRSGVSIQIVGIMADG
                     KHFTLSEDPKAAAFFPISQKGDIRTALIVRTQRDTGDMVATLRKVVRDLDLAVPIQES
                     GPWNSQLALSLFPSQVATVALGLFGAFGLLLSITGTFGLASYSVSKRLRELSIRVALG
                     AQAKQILSAALGRMLILLASGSVVGLVLGVAASRILSAIVYQASAQDPFVLAAVAFTV
                     LLTGSLSVAGPVRRVLHVDPANLLREQ"
     gene            6325..>6852
                     /locus_tag="VGR03_12890"
     CDS             6325..>6852
                     /locus_tag="VGR03_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995218.1"
                     /translation="MALYPPAAELELTPPSAYASPQKAMHCPYSFKRFLCLLAACAVL
                     PVAVWAQGITFISVSASPGLVSFRLAPSGVAPGSTPVSLTTQWRLRRGNVITLYAYFT
                     NPPAALSAGGASNIPSSHVSGTDASGRPLPFTGSGPFSNGASLTIFQVRIRGVNRRGQ
                     RTDAMSLQIDTTGLGL"
BASE COUNT         1280 a         2071 c         2061 g         1440 t
ORIGIN      
        1 agccgcctct ccgccgccat ctcccatgtc gtcagactcg aggaggcctg agcgcaacgc
       61 gcgcctaagg atatcctgtg cgcccacaac attggctgta caccatcccg cttcgcctgc
      121 gctccctctt tcgccgccgc cacgtcgagc aggagctgaa cgaagagctg cagtttcact
      181 ggagcacaaa atcgaggaag gcatcgccaa tgggctttcc ccaaaagagg cgcgctacgc
      241 ggcgatgcgc gcgatggacg gactggaaca acggaaagag gagatgagag acatgcgacg
      301 cattcattgg ttgacggatt ttctcgacga cgcgcgctac gcgttccgga gcctgcgccg
      361 cacgtccgga cttaccgcgt tcgtggtgat cacgctcgcg ctggggatcg ggatgacttc
      421 gacgcctttc agtatgctcg atgcgctgat cttcaggccg taccccgtgc cgcatccgag
      481 cagcgtggtc agcctggtga gcacttcaca cgacaatagc ttcgacaatt tctcgtatcg
      541 cgaatacctg gacatccgcg acaacacgag gagctacgac ggcgtgatcg caaacggaca
      601 gttgggggct gtcggcttca gcgccgagcc cgaagccacg ccgcgggtca ggggcggaat
      661 gatggtttcc ggtaactact tccaggtgct cggggtggag ccgcagctgg gccgcggttt
      721 tcgggaggat gaagatgagg tgccgggtcg cgatgccgtg gtggtgctcg ggccggactt
      781 ctggaagcac gaattcgcga gcgacccttc cgtcgtcggc aggattattc gcctgaacgg
      841 aacggacttc accgtgattg gcgtcgcgcc agaaacgttc ccggggatgc aaatattctt
      901 gcgtcccgac ttctacatgc cgctggcgat ggcgcgggtg ttttcgacca acccgcagaa
      961 gaattttctc gtggaccgag atgatcgcga gttgagtgtg aaggcgcgct tgaaacccgg
     1021 cacgacgctg cggcaggcgc gaaatgaact cgccgtgctc gcgcgagact tcgaacgcga
     1081 gtatccaaag gtcaatcgcg accgcggcgc ggcggtacgc acccagttcg aaatgcggac
     1141 gcaagatgat gacgtcaatt ggaaattcgg cgtgatcttt acgatccttg cgctggctgt
     1201 gctgctggtg gcgtgcacca acgttgcggg acttcttttg agccgagccc gcacgcgaac
     1261 gcgcgaaatc gctgtacggt tggcgatggg cgccgggcgt ttccggctga tccggctgct
     1321 cttgaccgaa agcctggttc tcgcgtgctt gggtggattg ggcgggatcg ccgtcgggta
     1381 cggcgccata aagttcctca gcactttcag aattccaacc gagttgccgg tcacgatccc
     1441 tttccgaatg gacacgcggg tgctgctggc aagcctcgcg ttgtcggtct tgagcgccct
     1501 tttatgcggc ctggcgccgg cactgcagag cacgcgcgtg gacctggtga acggactcaa
     1561 gtcggccgac gtcgacgtgc ccggacgcaa acgcctgtgg ggccggaatg tgctggtggt
     1621 ggcgcaggtt tccacgtccc tgatgctgct gacggcgtct ttcctgatgg cccggagttt
     1681 ccaacatagc ttgctggatg gaatcggttt cgcgaaagac catctgctga tggccaggtt
     1741 cgatccgcgt ctggtgcagt acaacgcggc tcagacgcgg cagttctaca agcttctggc
     1801 cgaacgtgtg cgggtagcgc ccggagttca gagcgcggct ctcacgcaga accctccgct
     1861 cggactgaac aactttgacc gcgtcgcctt cgtgccggac ggttttcaga tgccgcgcga
     1921 tcgcgagaac ttcacgtcga ccatggacac cgtggatgaa gggtacttcg agacgttggg
     1981 gataccgatc atgcgtggcc gcggcttcct ggcgtccgat accgcggatg cgccgcgtgt
     2041 cgccgtcgtg aatgagcagt tcgcgaaaca ctattggcct ggcgccgatg cagtgggcaa
     2101 acacatccgg cttgatagcg gcgtcggcac gccggttgag attgtcggcg tcgcgcagac
     2161 catcaagtat cgagagacca ccgaaaggcc gacggatttc gtgtatatgc cgttggccca
     2221 gcatcctgtc gcgcgaatgg tcctgctggt gcggtcgagc ggcgatccgc tgcaattggt
     2281 caagccagtg aagaacattg tccggacgct cgatccgaac ctgccgatgt tggagacgag
     2341 gacttatgag gacttgtacc ggtaccacgc cgtggaaggg ccgcgagttg cgatagaact
     2401 ggtcggtacc ctgggcgcgg tgggcctcct gctggccatc gccggtttgt acggactggt
     2461 ggcgtacaac gtgagccgca gaacccgcga gatcggcatt cgcatggccc ttggcgcggg
     2521 accgtctgac gtgctgcgcc tggtgatggg caagggcctc atgctggtgg gcatcggaac
     2581 ggtgatcggg ctggcgatgg gtttcgccgt cgagcagctc atgaattcca tgctgttcaa
     2641 tgccggcggc gtagacattg tggcttatgt catcgtcgtg ccgtcgatgt ttctggtgac
     2701 catgctggcg gcctacgtgc ccgcgcgaag agcatcccgg attgcgccaa tacaggcgct
     2761 gcgatacgag tagtgcagtt agttcgtgaa cgccagatcc gctacgcacg cttgccgaaa
     2821 gcacacaatg ctctaggcgc tgctcggcaa gaaacctcat aacatcatgg cgagtcaccg
     2881 attttaacgt tctgcgcaac aaggcgctgc aaggtttcag gccgcgttgt tcgtataccg
     2941 tagatggcga aatatcccgc cacaagcagg cagaggaggc agttcccagt ctagcccact
     3001 tctccgtcaa gtccggataa acgcgcttat cggacatgtc cggttatccg acttatcgtc
     3061 cactggcatg ctcgtttcct attgacactc tatacgtaga tcagtattat ccctatagag
     3121 agccgatagg aggcataaat tgagccctga gcagaccagc cttttgcaag ggactttgga
     3181 cctgctgatc ttgaaatcgc ttatcgctgg cgagatgcac ggattgggaa tctcgcgcag
     3241 gatccagcag atcacaggcg gcacttttgt tgttaagcca ggctcgctct ttcccgctct
     3301 acaccgaatg gacgaagagg gttggatctc ctccttttgg ggagactccg aaaacaaccg
     3361 ccgcgcgaaa tactaccgcc tgaccaaacc tggacggaac caactcgaag tcgaaaccaa
     3421 gcgatggggc cgcatttctt gggctatcgc tcaagctttg gaagcatcgt agggaggtac
     3481 ttagcatgcc gctcttggtg aaagcccgaa gtttcctgcg aaatctcttt ttatctcggc
     3541 gtgtggaggc agacctcgac caggaggttc attcccacct cgaaatgctg atcgaggaaa
     3601 acattcgcgc gggcatgcca ccgaaggaag cgcaacgcgc cgcgcggatc gaacttggcg
     3661 gagtcgagca agtcaaggag caagtgcgcg atgcgcggac gggcgcgttt ttcgattccc
     3721 tgttgcagga cctgcgattc gccctacgcc agatgcgccg ttctcccggc tttgccctca
     3781 ccgcggtctt caccctggcg ctcggcatcg ccgccaatgt catcgttttc ggcgtcctgc
     3841 aggcattagt cctgcggacc ctggatgtgc ctcacggaga ccgggtgatg acgcttcaac
     3901 ccaaagacgg tggccccttt ctttcgtatc cggaactgcg cgatgtgcgc gatgacaata
     3961 cggtgttttc tgccgttgcc gctcttgaaa taaacgaatt tggcttggaa gccaacggcg
     4021 tcacccggcc ggtctgggga tacgaagtga gcgggcagta cttcgaggtg gtcggcatca
     4081 agccgttcct ggggcggctg ctgcagcgcg ccgatgacga tcatcccgga gcctcggagg
     4141 ctgccgtgct ttcctggcca gcctggaaga gtgattttgg cgcggacccc aacatcgtgg
     4201 gaacgacggt gcgcattgac aagcatccgt acacgatcgt cggcgttacc ccggaagcct
     4261 tctacgggac cgaaaaattt ggcctggtcg acattttcgt tccaatggcg aatcaagcat
     4321 cgcttgacgg ggtcgactgg ctggagtcgc ggagccataa gaatctcttt tcgatcgtgc
     4381 gcatcaagga cggggtcacc atgccccagg tacaggctga attgaacacg attgttgccc
     4441 gcatggcccg gcagtatccg aaggaggaag aggggttggc attcaagctg gctcgtcctg
     4501 gtctggtcgg cgactttata ggcgggccga tgcgcggttt tctggcaggg gtgatgggac
     4561 tggccggcat tgtgctactc gccgcctgcg ccaacctcgg cagcctgttc gcagcgcgta
     4621 ccgcagaccg cacgcgcgag attgccatcc gcatggccat tggagcgagc cgctggcgga
     4681 tcctccgcca ggtccttgtc gaggcctttg tgatttcgat cttcgggggc gcgtgcgcct
     4741 gcggcctatc gtggatcgcc ctcaccggcc tggcgagctg gcatccgccc tccagattcc
     4801 ctttcaagtt ccatgtgctg ccgcagccat cgttgattct gatagcgttt cttatctcgg
     4861 tgttcgccgg cgttctgttc gggatgatgc cactgcggca gatattcaag accgatccta
     4921 acgatgccat caagagcggc ggaagccaat cctccgcagg acgccgctgg gcgcttcgcg
     4981 atgtgctgct ggcggcacag atcgcgctct gctgcgtcac cgtcacggct gcctttgtct
     5041 cgctgcgtgg cctgagcaaa gcactgacta tggacctggg tttcaatacg aagaatgctg
     5101 tgcgtacgac attaaatctg agccaggctg gctataccac agattccgcc gatcctttcc
     5161 agcgccagct actggaaagg gtctcgcagc tcccgggagt ggaagcagca ggctacgcga
     5221 ataccacgcc cctcgacttc gacgcgtcca tcactgctgt cttctctcaa caaactaccg
     5281 atttcagacc gtcaaacaaa gcatttgagg cctattggta cgacgtctca cctggatact
     5341 tgaccgccgc gggaacacca ctgctcgcgg gacgcgatgt aagctttacc gacactgcca
     5401 agacgccggc tgtggccatt gtgaatcagg agttcgcccg gcggctcttc cactccgagg
     5461 acgccgtcgg ccgctattat aaaaaccgct ccggtgtgtc gatccagatc gtgggcatca
     5521 tggcagatgg aaaacacttc acgctcagtg aagatccgaa agccgcagcg ttcttcccga
     5581 tctcgcagaa aggggacata aggacggcgc tcatcgtccg gacgcagcgc gacacgggcg
     5641 atatggtagc cacccttcgc aaagtggtcc gcgatcttga cctcgccgtc cctattcagg
     5701 aatcgggccc ctggaacagc cagcttgcac tgagtctctt tccatcccag gtggctacag
     5761 tggccctcgg cctctttggc gcttttggac tcctgctctc gatcaccggc accttcggcc
     5821 tggcctccta ttcggtcagc aagcggctgc gcgagctgag tattcgtgtc gcgctcggtg
     5881 cgcaggcgaa gcagattctc tccgcagcgc tcggccgtat gctgatcctg ctcgcgagcg
     5941 gctctgtggt tggccttgtg ctgggcgtgg cggccagccg aatactctcc gccatcgtct
     6001 accaagcctc cgcgcaggat ccatttgtgc ttgctgctgt tgcatttact gtgctgctca
     6061 ccggatcact ttcggtcgcc ggtcccgtga gacgcgtcct gcacgtcgac ccggcaaacc
     6121 tgctccgcga acaataattt tcaagccaga tttggccttc atatttgaat gtgcacgagt
     6181 gacgcgcacc cggaagtaat ccacatgcct gattaacgcg ctaaccggac aagtgcacct
     6241 tgtcgcccca gaagtccgct tctgggaagc tgcaacaccc tggctgtcga tcacttgtcg
     6301 gtatacgcgg cgttccggaa actgatggcc ctctacccgc cggccgccga acttgaattg
     6361 accccgccaa gtgcctatgc ttctccccag aaagcaatgc attgccccta cagtttcaag
     6421 cgtttcctgt gtctcctggc cgcatgcgcc gtgctgccgg tggccgtgtg ggcccaaggc
     6481 atcacgttca tcagcgtatc agcctctccg ggcctggtga gcttccgcct cgctccgagc
     6541 ggcgtggccc cagggagcac gccggtcagt ctgaccacgc agtggcgcct acgccgtggc
     6601 aacgtgatca cgctctatgc gtatttcacc aatccccctg ccgcgctctc cgccggcggc
     6661 gcgtccaaca tcccttcctc ccacgtctcc ggcaccgacg catccggtag acctctcccc
     6721 ttcaccggct ccgggccgtt ctcgaatggc gcaagcctga ccatctttca ggtccgcatc
     6781 cggggcgtaa accgcagggg ccagcgcacc gacgccatgt ccctgcagat tgacaccact
     6841 gggctggggc tg
//
LOCUS       DASYVO010000382         3845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_276947, whole genome shotgun sequence.
ACCESSION   DASYVO010000382 DASYVO010000000
VERSION     DASYVO010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276947"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2320
                     /gene="ppdK"
                     /locus_tag="VGR03_12895"
     CDS             <1..2320
                     /gene="ppdK"
                     /locus_tag="VGR03_12895"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEV1995219.1"
                     /translation="TRNERFAYDAYRRFVQMYSTVVMGMSKEDLEHRLRSMKDRLGVK
                     EDTAVSASGWKELVAEYKAYFRKESGSDFPDHPIDQLWGAIGAVFGSWMAEKAVTYRR
                     VEKITGLLGTAVNVVQMVFGNTGDTSGTGVCFTRDPSSGEKIFYGDYLVNAQGEDVVA
                     GIRTPMPLGEMQKTMPKVYGQLEKVRVRLEKHYRDMQDMEFTVEDGTLYMLQTRTGKR
                     TPSATFRIAVDMANEKLITKEEALLRVKPEDIERLFYPVIDAKVDKKSLVSKILATGI
                     NAVPGAAVGKVVFSAQKAEEWAERGEKVILVRRETSPEDVGGMAVAQGILTATGGKTS
                     HAAVVARGWGKCCVVGAEKLDIDYKSLSMAASGKVVKEGDWITLDGSEGVVYTEAMPL
                     VVPQLPKSYETLMKWADETRRLRVRTNVDTPQDARKAIEFGAEGIGLCRTEHMFFTDF
                     EQPERSHERQRAIQEMILADTREARVKALDKLLPFQRRDFVGIFKAMDGLPVTIRLID
                     PPLHEFVPHERAKQEELARDLNIDVQTVVRRVEQLHEANPMLGHRGCRLAITYPEILE
                     MQVRAIIEAAIECQRGRVKVLPEIMHPLVLDRKELAILEQATRRVADDLIKKSGIKLN
                     YLVGTMIELPRAALLAGEIAEVAEFFSFGTNDLTQTTMGLSRDDAGRFLPDYVDEKKA
                     AVFSADPFQSLDTKGVGMLMRWGIEQGRKTRPKLKIGICGEHGGDADSVKFCHQVGMD
                     YVSASPFRVPISRLAAAQVVVEEKQAKKKKGK"
     gene            complement(2475..>3845)
                     /locus_tag="VGR03_12900"
     CDS             complement(2475..>3845)
                     /locus_tag="VGR03_12900"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEV1995220.1"
                     /translation="SVMGAGFARQGTLTPAAGIWIANAVLFALGLTLLPRMEQFRGEN
                     RWFRPILYLQSRMRLLRRRRAQARAKAATANGVNGERVSQEILSRSSGSFPQLIDIYV
                     LRRFFSYFALLMGAFVFLFETFTFFELLDDIARHRVPFLIVVDYFRYLAPYLLYQLAP
                     LGALVAVLVTLGIMSKNNEIVAIKASGISLYRLAVPLLLAGVALAGIMIVLDDTYLPY
                     ANQRQDALHNLIKGRPPQTYTHPHRWIFGESSKIYNYDLFDPKQNLFGGLTVLEIDPG
                     TFQVRRRIFAPRARWSETPKVWVLEGGWVRDFADGEIVRYERLPPVTSLPELTEPPSY
                     FNPDVKQGFQMSWRELGSYIGGLQRAGFDVSTLTVQWHEKIAFPLLAPVSMLLAIPFA
                     FLVGTRGAVGGVALGVGFGIVYWSVAKLLEAMGGVGQLPPFLAGWSPDIIFFFLGMYF
                     FFKMPT"
BASE COUNT          891 a         1168 c         1084 g          702 t
ORIGIN      
        1 aaccaggaac gagcgtttcg cctacgacgc ttaccgccgc ttcgtgcaga tgtattcaac
       61 cgtcgtcatg ggcatgtcca aggaagatct tgaacaccgc cttcgctcta tgaaagaccg
      121 cctcggcgtc aaggaagata ccgccgtctc cgccagcggc tggaaagaat tggtagcgga
      181 atacaaagcg tactttcgta aagaatccgg gagcgacttt ccggaccacc ccatcgacca
      241 gctttgggga gcgatcggcg cggtgttcgg ttcctggatg gcagagaagg ccgtcaccta
      301 tcgccgcgtc gagaaaatca ccggcctcct cggcacggcc gtcaacgttg tccagatggt
      361 cttcggaaat accggcgaca cttccggcac cggcgtctgc ttcacgcgcg atccgagttc
      421 tggcgagaaa atcttctatg gcgactacct cgtgaacgcg cagggtgaag acgtggttgc
      481 cggcattcgc acccccatgc ctttgggcga aatgcaaaag accatgccta aggtttacgg
      541 acagctcgag aaagttcgag taagacttga gaagcactat cgcgacatgc aggatatgga
      601 gttcaccgtc gaggacggaa cgctctacat gctgcagacg cgcaccggta agcgcacgcc
      661 ttccgccacc tttcgcatcg cggtcgacat ggccaacgaa aaactcatca ccaaagagga
      721 agcccttctc cgtgtcaaac ctgaagacat cgagcggctc ttttatcccg tgatcgatgc
      781 aaaggtcgac aagaaatccc tggtctcgaa aatcctggct accggcatca atgccgtccc
      841 tggtgcagca gtcggcaaag tcgtcttctc cgcgcagaaa gcggaagagt gggccgagcg
      901 cggcgagaag gtcatcctgg tccgtcgcga aaccagcccc gaggacgtcg gcggcatggc
      961 cgtcgcgcag ggcattctga ccgctaccgg cggcaagacc agccacgccg ccgtcgtcgc
     1021 ccgcggctgg ggcaagtgtt gcgtggtcgg cgctgaaaaa ctagacatcg attacaaatc
     1081 actttcgatg gccgccagcg gcaaggtcgt caaagaaggg gattggatca cgctcgacgg
     1141 cagcgaaggc gtggtttaca ccgaagcgat gcccctggtc gttccccagc ttcccaaatc
     1201 ctacgaaacg ctcatgaagt gggccgatga gacgcgccgc ctcagggtcc gcacgaacgt
     1261 cgacactccg caggacgctc gcaaggccat cgagttcggg gcggagggca ttggcctctg
     1321 ccgcacggag cacatgttct tcaccgattt cgagcagcca gaaagaagcc acgagcgtca
     1381 gcgcgccatc caggaaatga ttctcgccga cacccgggaa gctcgcgtca aggccctcga
     1441 caagttgctt ccgttccagc gccgcgattt cgtcggcatc ttcaaggcca tggacggcct
     1501 cccggtcacc atccgcttga ttgacccgcc gctccacgaa ttcgtcccgc atgagcgtgc
     1561 caagcaggaa gaactggcgc gtgatctgaa tatcgatgtc caaactgtgg tgcgccgcgt
     1621 cgagcagttg cacgaagcca accccatgct cggccatcgc ggctgccgcc tggccatcac
     1681 ctatcccgag attctcgaga tgcaagtgcg cgccatcatc gaagcggcca tcgaatgcca
     1741 gaggggaaga gttaaagtat tacctgagat tatgcatccg ctcgttttgg atcgcaagga
     1801 actcgccatt cttgaacagg cgacacgccg cgtcgccgat gacttaatca aaaagtccgg
     1861 gatcaaacta aactatctcg tgggaacgat gatcgaactc ccccgagccg ctctgctcgc
     1921 cggggaaatc gccgaagtgg cggaattttt ctcgtttggc acgaacgacc tgacacaaac
     1981 caccatgggc ctctcgcgcg acgatgccgg ccgcttcctg ccggattacg tggacgaaaa
     2041 gaaagctgcc gttttcagcg ccgatccttt ccagtccctg gataccaagg gcgtcggcat
     2101 gttgatgcgc tggggcatcg agcaagggcg caagacccgc cccaaactca agatcggcat
     2161 ctgcggcgaa catggcggcg acgcggacag cgtgaagttt tgccatcagg tgggtatgga
     2221 ctacgtcagc gcttctccgt tccgcgttcc tatttcgcgc ttggcagcag ctcaagtagt
     2281 agttgaagaa aaacaagcca agaaaaagaa ggggaagtaa tcgagaactc ggttcgaacg
     2341 aacttaaagt aacacttgaa gccgtcggcg cgcagacatt tcgcaatctc tgctcgctcg
     2401 acggctcgcg gttttgtccg cgattcacga tctgaacgtg acagtgaacc ttccacgcgg
     2461 tactatcggc tctcttacgt gggcatcttg aagaagaagt acatgccgag gaaaaagaaa
     2521 ataatgtcgg gcgaccagcc cgccagaaac ggcggaagct ggccaacccc gcccatggcc
     2581 tccaagagtt tggccaccga ccaatagacg attccgaatc ccactccgag agccacgcct
     2641 cccaccgcac cgcgcgtccc caccagaaac gcgaacggga tcgccagcag catgctgacc
     2701 ggcgcaagca gcgggaaggc aatcttttcg tgccattgca ccgtcagagt cgagacgtca
     2761 aagcccgcac gctgcaaccc gccgatataa ctcccgagtt cccgccagct catctggaac
     2821 ccctgtttaa catccggatt gaagtagctg ggcggctcgg tgagctccgg gagcgaggtg
     2881 accggaggaa ggcgctcgta gcgcacaatc tctccatccg caaaatcgcg cacccacccc
     2941 ccttcgagga cccaaacttt tggggtttcc gaccaccgtg cgcgcggtgc aaagattcgg
     3001 cgccgcacct gaaacgtccc cggatcgatt tcgagcaccg tgagcccgcc aaacaggttt
     3061 tgtttggggt caaagagatc gtagttgtag atttttgaac tttccccgaa aatccaccga
     3121 tgcgggtgag tgtaggtctg cggcggacgg cctttgatca ggttatggag cgcgtcctgc
     3181 cgctggttcg catagggcag gtaggtgtcg tcgagcacga tcataattcc agccagggct
     3241 acccccgcaa gaagcagcgg taccgccagc cggtacaaac tgatgccgct ggccttgatg
     3301 gccacgattt cattgttctt gctcatgatc cccagcgtca ccagaactgc gaccagagcc
     3361 cccagcggcg cgagctggta cagcagatac ggagcaaggt agcggaaata atcgaccacg
     3421 atcagaaatg gaacgcggtg gcgcgcaatg tcgtcgagta gctcgaagaa cgtgaaggtc
     3481 tcaaatagaa aaacaaacgc ccccatcagc agcgcaaaat aagaaaagaa tcgccgcaaa
     3541 acgtagatgt cgatcaattg cgggaaactt ccgctggacc gcgacaggat ttcctgtgac
     3601 acgcgttcgc cattcacccc atttgccgtg gcagctttgg cgcgagcttg ggctctccgc
     3661 cgtcgcaaca gtcgcattcg ggattgcaaa tagaggatcg gccggaacca tcggttttcg
     3721 ccgcggaatt gctccattcg cggcaagagc gtcagcccca gcgcgaaaag aacggcgttg
     3781 gcgatccaga ttcccgcggc cggcgtcagc gtaccttgcc gcgcaaaccc cgcccccatc
     3841 acgga
//
LOCUS       DASYVO010000383         5110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_65005, whole genome shotgun sequence.
ACCESSION   DASYVO010000383 DASYVO010000000
VERSION     DASYVO010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65005"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VGR03_12905"
     CDS             complement(<1..662)
                     /locus_tag="VGR03_12905"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV1995221.1"
                     /translation="MTTADGFWAGKTVFVTGGASFIGSTLTDQLLARGAKKVRIVDDL
                     SSGHLDNIRQHLGTGKVDFLQADLREPGVTRAAMQGVDTVFHLAADHGGRGYVDLHQA
                     GPASNFFLDGLVFAEALKAKVKKVVFASSGCVYPNFLQSDTKKELYLTEDLTKGPNDA
                     DNTYGWAKLMGEFTLQAYAKEHGMGAASCRYFTVYGPRGVENHAVIAMIARAFLGLNP
                     FEV"
     gene            complement(686..1834)
                     /locus_tag="VGR03_12910"
     CDS             complement(686..1834)
                     /locus_tag="VGR03_12910"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1995222.1"
                     /translation="MAHSVAEAISISHLVTVLCGRPSYDPTERRPWRLFQTEQTGLVR
                     IIRVGSTDFPRFYMKKRVMNYLSYVALAIPRALLVPCDAVVAMTDPPFQGIAGAIVAM
                     LKRKPYVYNIRDMYPDMAVGGSIVEPGRLARIWEKLHRWALRRATRVVVLGEDMRARI
                     IAKGVEPARVLIVRDGTEILPPNTPLPAPDPEVVRAIRGNFSFVLVHAGNLGFYGAWN
                     TLVTAARNLASEGVSLVFVGDGAQRSQIEAAAAGSSNIRFLEFFPASKIPSVLAAADA
                     HVITVKRGLEGVVVPSKMYGILAAGKPIVAVAPKETDVVSLGLQRGFAVAADPDRPAE
                     VVSAVRALASDPNRLKTMGEAARAAAPDYDRVKELQKFVEIIDHLTVV"
     gene            complement(2235..2552)
                     /locus_tag="VGR03_12915"
     CDS             complement(2235..2552)
                     /locus_tag="VGR03_12915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEV1995223.1"
                     /translation="MSKPQSLMHPVHPGEILRKDFMKPLRLTVNKLALDLRVPATRIG
                     EIVHERRRITADTALRLARYFNNNPEFWLNLQNFYDLEVSRRAGAASEIERHVHPAAA
                     PVS"
     gene            complement(2977..4725)
                     /locus_tag="VGR03_12920"
     CDS             complement(2977..4725)
                     /locus_tag="VGR03_12920"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HEV1995224.1"
                     /translation="MNILGINAYHGNASAAIVCDGRLIAAVEEERFNRVKYAAGFPAQ
                     AIRFCLKEAGLTLQDIDHVAVPRNPYARLGTKLFYALRMPSFARERVKVLAKFTAIPE
                     ALASVFDADPKKISAKFHRVEHHQAHLASSFFVSPFERAALLSADGLGDFASTMWGAG
                     EGNRMRIDGAVAFPHSLGLFYSAVTQYLGFLKFGDEYKVMGLAAYGHAEQLDAFRDIV
                     RFDSQSHSNGFRLGLDYFLHHRTGPEISWAEADKTPTLGKMFSDEMAKRLGPVRAPEE
                     ALEERHRNLACSLQARLEEVYLGMLKKLGERTGLKAVCLAGGVAFNCVANGKIFDATP
                     FEQVYVHPAAGDAGLAVGAAYYVWHQKLGKPRSFVMNHAYWGPGYSREEIRRAIDANG
                     LAQSGYSIAELPEEELPRRAAAIIADGKILGWFQGRAEWGPRALGNRSIVADPRRPEM
                     KEILNRRIKHREIFRPFAPSILAEATGEYFEKSHPSPFMTLAYSVRPEKRDKIPAPTH
                     VDGTGRLQTVRGEANPRYYALISAFRDLTGVPVVLNTSFNDNEPIVCRPEEAIDCFLR
                     TQMDALVLGDFLISRP"
     gene            complement(4759..>5110)
                     /locus_tag="VGR03_12925"
     CDS             complement(4759..>5110)
                     /locus_tag="VGR03_12925"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEV1995225.1"
                     /translation="LDTPLVEFVESLPDAARRRPGAQKALLVEALGDLLPQEILAQRK
                     RTFTLPWEEWLRGPLRARMEASFADPAPALAAYLRPGGVRAVWTDFLAGKTTWSRPWS
                     LYVLNEWCHRHLAA"
BASE COUNT         1042 a         1526 c         1534 g         1008 t
ORIGIN      
        1 acttcgaaag ggttcaggcc gagaaaagcg cgcgcaatca tggcgatgac ggcgtgattc
       61 tccaccccgc gcgggccata caccgtgaag tagcggcagg aagcggcgcc cattccgtgt
      121 tccttcgcat aagcttgcag cgtgaattcc cccatcaact tggcccagcc gtaggtgttg
      181 tcggcatcat tcgggccttt ggtgaggtct tccgtgagat aaagctcttt cttggtgtcc
      241 gattgcagaa aattcggata aacgcagccg gaagaagcga acaccacttt ttttactttg
      301 gctttgagtg cttcggcaaa gaccagcccg tccaggaaaa agttcgacgc tgggccggct
      361 tggtggaggt ctacgtagcc gcgcccgccg tggtccgccg cgagatggaa aacggtgtcg
      421 acgccctgca tcgcggcccg ggtcacaccg ggttcccgca aatcggcctg cagaaaatcg
      481 accttgcccg tgccgaggtg ctggcgaatg ttgtccagat ggccgctgct caggtcatcg
      541 acgatgcgaa cctttttcgc gcctcgcgcc aggagttgat cggtaagcgt cgagccgatg
      601 aacgacgctc cgcccgtcac aaaaactgtt tttcccgccc aaaatccgtc cgccgtcgtc
      661 acgcttgtgt tgcctcctag attttttaaa caaccgtcag gtgatcgatg atttccacga
      721 atttttgcaa ttccttgact ctatcataat cgggagcggc ggcccgtgcc gcttcgccca
      781 tggtcttcag cctattcggg tcggaggcca gcgcgcgcac agcgctgaca acttcggcgg
      841 gccggtccgg atcggccgct accgcgaaac cacgctgaag ccccaacgac acaacatccg
      901 tctctttcgg cgccaccgcg acgatgggtt ttcccgccgc cagaatcccg tacattttgc
      961 tgggcaccac cacgccttcc aggccccgct tcacggtaat gacgtgagca tcggcggccg
     1021 cgagcaccga cggaatcttg ctcgccggga aaaactccag aaaacggata ttgctggaac
     1081 ccgcagccgc agcttcgatt tgtgagcgct gcgcgccatc gcccacaaac acgaggctca
     1141 caccttcgct cgcaaggttg cgcgcggcgg tcaccagcgt gttccacgcg ccgtagaatc
     1201 caaggttgcc cgcgtgcaca agaacaaacg aaaaattccc gcggatcgcc cggacaacct
     1261 cgggatcggg ggccggaaga ggcgtattcg gcggaaggat ttcggttcca tcgcgaacga
     1321 tcagcacccg ggcgggttcc acacctttgg cgataatgcg ggcgcgcatg tcctcgccga
     1381 gaacgacaac gcgcgtggcg cggcgcagcg cccagcgatg gagtttttcc cagattctcg
     1441 cgagcctgcc gggctcaacg atcgagcctc ctaccgccat gtccggatac atgtccctga
     1501 tgttgtagac gtaaggcttg cgtttcaaca tggcgacgat cgcgccggcg attccttgaa
     1561 acgggggatc ggtcatcgcc acgacggcgt cacagggcac caagagcgca cggggaatcg
     1621 ccagcgcgac gtagctgaga taattcatca cgcgcttttt catgtagaag cgcggaaagt
     1681 ccgtcgaacc cacgcgaatg atgcgcacca aaccggtttg ctccgtttga aagagccgcc
     1741 acggccggcg ctcggtggga tcgtaggacg gccgcccgca cagcaccgtc acgaggtggg
     1801 aaatggaaat agcttccgca acgctgtgtg ccattttcgc ggtcgcggaa gtgtccggcg
     1861 ggtaatattg attgagcaac aggatgcgca cggaaagatt gtaccgagtt ccgtctctgc
     1921 gcggggcgcg gtcgcctaac agttttaggc gggttttttg aagatcggcg ccgccgcccg
     1981 ctgcagctcg tgcgcggtcg gcttcttttt tggcggcttc ttgacttcgc gtttgcctct
     2041 gtctttattt cccatgcccg atagcttgcc acaattcgct agctctggct agtggccgta
     2101 aattccaaac cggacaatta agtgtcacga gtgggaggtc acgaagtcgc cgcaagcctg
     2161 gacttccatt tgcctgcgga tgattaagag gtgacagagg atgagccgaa tttcacccgg
     2221 cttgggcacg ccgatcaaga gactggcgcg gcggcgggat gaacgtggcg ctcgatttcg
     2281 gaggcggcgc cggcacgccg ggagacttcc agatcgtaaa aattctggag gttaagccag
     2341 aattcggggt tgttgttgaa gtagcgggcg aggcgaagcg cggtgtccgc ggtgatgcgg
     2401 cggcgctcgt gcacaatctc accgatgcgc gtggcgggca cgcgcagatc cagcgccagt
     2461 ttgttgaccg tcagccggag cggtttcata aaatctttgc ggaggatttc gcccgggtgg
     2521 acgggatgca tcaatgattg cggcttgctc atgtgcagtt ctctcctagt gataatcgac
     2581 aatttccacg gcatggaaac ggccatcacg ccaaataaaa cagattcgcc attgatcgtt
     2641 gatgcgaatc gagtgttggc ctttccgatc accccgtaac gcttccaatt gatttccagg
     2701 cggtgcagcc aggaaccgaa gttccgtcgc cgcgtgcaat tggagcaact tgcgcagagc
     2761 gatgcgctcg aatgcacgga agcgcttgga gcgatgatag tgaaaaagcg cttcagtttc
     2821 cttgcagcgg aagctctgga tcacacgtct aggatactac gtgatacgtt atacgccaag
     2881 cgaaatgttc gcagggtcag cagggagcag gagcttgggc cgcgctccgg gctggccctc
     2941 gcagttccca ggacatcgtc gtggctggct gcggcactaa ggccgggaga tgaggaaatc
     3001 gccgaggacc agggcatcca tctgcgtgcg caggaagcaa tcgatcgctt cttcggggcg
     3061 gcagacgatc ggctcgttgt cgttgaagga agtgttcagc accacgggca cgccggtgag
     3121 atcgcgaaaa gcggaaatga gcgcgtaata gcgcgggtta gcttcgcccc taacagtttg
     3181 cagccggccc gtgccgtcca cgtgcgtcgg cgccgggatc ttgtcgcgct tttccgggcg
     3241 cacggaatag gccagggtca tgaacggcga ggggtgcgac ttctcaaaat actccccggt
     3301 ggcttccgcg aggatggaag gcgcaaaggg gcgaaagatt tcccggtgct tgatccggcg
     3361 gttgagaatt tccttcattt ccggacggcg cggatcggcg acgatgcttc gattgccaag
     3421 tgcccgcgga ccccattcgg cgcggccctg aaaccagccg agaatctttc catccgcgat
     3481 gatcgccgcg gcgcgccgcg gcaactcttc ttcgggcagc tcggcgatgg aatagccgct
     3541 ttgtgccagg ccgtttgcat cgattgcgcg gcgaatctct tcgcgcgaat atccaggtcc
     3601 ccagtaagcg tgattcatca cgaaggaacg tggtttgccc aatttctgat gccagacgta
     3661 gtacgcggcg cccaccgcga ggcccgcgtc ccccgccgcc ggatgaacgt agacctgctc
     3721 gaacggtgtc gcgtcaaaaa ttttcccgtt tgcgacgcaa ttgaaagcca caccaccggc
     3781 cagacacacg gccttgagcc cggttcgctc gccaagcttc ttcaacatac cgagataaac
     3841 ctcttcgagc cgcgcctgca acgaacaagc gagattgcgg tgccgctcct ccagcgcttc
     3901 ctcaggagcg cggaccggcc ccaggcgctt cgccatttca tccgaaaaca ttttgccgag
     3961 cgtcggcgtc ttatcagctt cggcccagga tatttccgga cccgtgcggt gatgcaagaa
     4021 gtaatccagc cccaggcgga agccgttaga gtgggattgg ctgtcaaaac gcacgatgtc
     4081 gcggaaagcg tcgagttgtt cagcgtggcc gtaagcggcg agccccatga ctttgtactc
     4141 gtcgccgaat ttcaagaacc cgagatactg cgtcaccgcg ctgtaaaaca gccccagcga
     4201 atgcggaaag gctaccgcgc catcgatcct catgcggttg ccttcgccgg cgccccacat
     4261 cgtgctcgcg aaatcgccga gtccgtcggc ggaaagcaac gcggcgcgtt cgaacggaga
     4321 aacgaaaaag ctgctcgcga gatgtgcctg gtggtgctcg acgcggtgga actttgcgct
     4381 gatcttcttg ggatcggcat caaagaccga cgccagtgcc tcgggaatag cagtgaattt
     4441 cgcgagaact ttgacgcgtt cgcgcgcaaa agacggcatg cgcagcgcgt aaaaaagttt
     4501 tgtgcccagt ctcgcgtacg gatttcgcgg cacagcaacg tgatcgatat cttgaagcgt
     4561 gaggcccgct tccttcagac aaaaacgaat cgcctgcgcg ggaaatcccg ccgcgtactt
     4621 cacgcggtta aagcgttcct cctcgaccgc ggcgatcagc cttccgtcgc agacaatcgc
     4681 cgccgaagcg ttcccgtggt acgcattgat cccaagaata ttcatcgcgc gatttgtcag
     4741 tgtacctcag ccggaagatt atgcggcgag gtgtcggtgg caccactcgt tcaggacata
     4801 caaggaccag gggcgcgacc aagtggtttt cccggcaagg aaatccgtcc aaaccgcgcg
     4861 aacgcctccc ggacgaagat aggccgccag cgccggcgca gggtctgcga agcttgcctc
     4921 catccgcgcg cgcagcggcc cgcgcagcca ctcttcccat ggcagcgtaa acgtgcgttt
     4981 ccgttgcgcg agaatctcct gcggcagcaa atcgccgagc gcttccacca aaagcgcttt
     5041 ttgtgctcca gggcggcggc gcgccgcatc cggcagtgac tccacaaatt caaccagggg
     5101 agtgtcgagg
//
LOCUS       DASYVO010000384         3548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_249265, whole genome shotgun sequence.
ACCESSION   DASYVO010000384 DASYVO010000000
VERSION     DASYVO010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249265"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="VGR03_12930"
     CDS             <1..1089
                     /locus_tag="VGR03_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995226.1"
                     /translation="GTGGFTIFNTNDLLGGLDRIGREQNEFYILGYVPGDTPEGSCHT
                     LKVKLNRGGMNVRARSGYCNTRSVNVLEGKPLEKQLESHALGSQTGSIHGALQAPYFY
                     TAPETARVNLAMEIPADTFQFNKDKGKYHANLNVLGIAYRADGTVGARFSDTVNMDLE
                     KDEWKEFTKLPYRYENQFDATPGAYRLTVVLSAGGDAFGKFESPLAIDPYDGKHFSLS
                     GVALTNSAQRINDIPTGLDSVLLEDRTPLVVKGLQIVPSGSNRFKNTDKVALYAEIYE
                     PLLTSANPPLVAMAYHIFERSTNKEVFFTQLVRADEYIQKGNSVLPIGMKVKVEDLKP
                     GSYRLVMQAVDSVNNHAPDRSVDFDIVQ"
     gene            1361..3388
                     /locus_tag="VGR03_12935"
     CDS             1361..3388
                     /locus_tag="VGR03_12935"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEV1995227.1"
                     /translation="MRLQERPASPFMQRIPAISATPRSFLAAVLSVACVLSLTVELPA
                     SAAIPAAAKDKKPKTDPALKGLPITELSADEAILHALNRLAYGPRPGDIERVRQVGLA
                     KWIEQQLNPNSIDDRALDARLTDYPTLRMSTAKLMDEFPQPKQVEKQAEKQAQARTQQ
                     SQSRSDAATAVVEKDMRTVRGRPGNSDAPPATSVSAPQRPADNSNAPAPMKQQTQGNA
                     ATNGAVRREVLGGGDPNNVPRAIADDSKRPQRVVAELSMAKVTRAIYSNRQLQQVMDD
                     FWFNHFNVFAGKGEDRYYLTSYERDVIQPHAFGKFKDLVTATAKSPAMLFYLDNFLSA
                     DPRAAQRQAAERSMRQQRRRGGFGGPRPSAYPQQAKKNERGLNENYGRELMELHTLGV
                     DGGYTQKDVTEVARCFTGWTIEKPRQYPEFKFDDRLHDPDPKLVLGKKIHAGGMKDGE
                     QVIDLLVHHPSTAKFISTKLARRFVSDNPPPALVNRMAQTFQSSDGDIRAVLKTMIWS
                     PEFWSRESYRAKIKTPYELVVSAVRALGTDVGTPMPLVQWVGRIGEPLYQCQPPTGYS
                     DKADTWVNTGALLNRLNYSLALAGNKMRGARTDVTSLLGVEPSADPRAALDRAVQVFL
                     GGQAGATTVETLEKQLDNPQVLQAKLDDPRKQVDLGVVAGLVLGAPEFQRR"
BASE COUNT          794 a         1002 c         1083 g          669 t
ORIGIN      
        1 ggcacgggcg gtttcacgat tttcaacacc aacgacctgc tgggtggact cgatcgcatc
       61 gggcgagagc aaaacgagtt ctacatcctg gggtacgtgc ccggcgacac tccggaagga
      121 agctgccaca cgctgaaggt gaagctgaat cgcggcggaa tgaatgtgcg cgcgcgcagc
      181 ggatactgca atacgcgatc cgtaaacgtg ctcgaaggca agcccctgga aaaacagctc
      241 gaatcgcacg ccctgggctc tcagacaggt tcgattcatg gcgcgttgca agcgccctat
      301 ttctacaccg cgccagaaac ggcgcgcgtc aacctggcga tggaaattcc cgcggataca
      361 tttcaattta ataaggacaa gggaaagtat cacgccaact tgaacgtgct gggcatcgcc
      421 taccgcgcag atgggactgt cggagcgcgc ttcagcgata ccgtcaacat ggacttggag
      481 aaggatgagt ggaaagagtt caccaagttg ccgtatcgct atgaaaatca attcgacgct
      541 acaccgggcg catacaggct gacggttgtc ctgagcgccg gcggcgatgc gtttggaaaa
      601 tttgaatcgc cactcgccat cgatccgtat gacggaaaac acttcagcct cagcggggtg
      661 gcgctgacga attccgcgca aagaataaat gatattccga ccggactgga ttccgtgctg
      721 ctcgaagacc gcaccccgct ggtggtcaag ggcttgcaaa tcgtcccttc cggcagcaat
      781 cggtttaaga atacggacaa agtggctctg tacgcggaga tttatgaacc gctgctgacc
      841 tcggcgaacc cgccgcttgt ggccatggcc tatcacatat tcgagcgatc aacgaacaag
      901 gaagtgttct tcacccaact cgttcgtgcc gacgaataca tccagaaagg gaattccgtg
      961 cttcctatcg ggatgaaagt gaaggtggag gacctgaaac cgggctcgta tcgcctggtg
     1021 atgcaggctg tcgacagcgt caacaatcat gcgccggatc gcagcgtgga cttcgacata
     1081 gtccagtgat ggatctcccg cagcactgaa aaatcgacgg acgcgttagg gagcgaggga
     1141 gctctgctcc cgctctgact gttgcttcca gactgaaacc gtgaaagcgg cggtgagccg
     1201 ccacgctctg caacagcccc taagtccttt tctgaatgta ctttcggccg tggtaaggat
     1261 tcgacacggt ttctggactt gtgcaacttc ttatcctcgc aagggttatt ctattcatgc
     1321 taggtaattc gcccaatatt ctacgcggca ggagagtgcc atgaggctgc aagagcgccc
     1381 cgcctcacca tttatgcaac gtatccctgc gatttccgcg acgccgaggt cattcctcgc
     1441 cgccgtcctc tctgttgctt gcgttttgtc gctcactgtc gaattgccag ccagcgccgc
     1501 aatccccgca gcggcgaagg ataagaaacc caagaccgat cctgcgctca aaggcttgcc
     1561 gattacagaa ctcagtgccg acgaagccat tttgcatgcg ttgaaccgcc tcgcctacgg
     1621 cccgcgcccg ggcgacattg agcgcgtccg gcaggtgggt ctcgccaagt ggatcgagca
     1681 gcagctcaat cccaattcca tcgatgaccg cgcgctggat gcacgcctaa cggactatcc
     1741 gacgctgcgg atgtccaccg cgaaattgat ggatgaattt ccccagccca agcaagtgga
     1801 aaagcaagct gagaagcagg cccaggcacg aacgcagcag agccaaagcc gatccgatgc
     1861 ggccactgcg gtcgtcgaga aagatatgcg tactgtgcga gggcggccag gaaattcaga
     1921 tgctccgcct gccacttcag tttccgcgcc gcaacgccca gccgacaatt ccaacgcgcc
     1981 ggctcctatg aaacagcaaa cgcaggggaa cgcggcgacg aatggcgcgg tgcggcgcga
     2041 agttttgggc ggtggcgacc ccaacaatgt tccgcgggcg attgccgacg acagcaagag
     2101 gccgcagcgc gtggtggcgg aactgagcat ggccaaggtg acgcgcgcga tttacagcaa
     2161 ccggcaactc cagcaggtga tggacgattt ctggtttaac cacttcaatg ttttcgcggg
     2221 caaaggtgaa gaccgctact acctcacttc gtatgaacgc gacgtgattc agcctcacgc
     2281 cttcgggaaa ttcaaagatt tggtgaccgc gacggccaag agccccgcga tgctttttta
     2341 tttggacaat tttctgagcg ccgatccgcg ggccgcgcag cggcaggcag ccgagcgaag
     2401 catgcgccag cagaggcgcc gcggcggatt tggcgggccg cgtccatcgg cctacccgca
     2461 gcaagcaaag aaaaatgaac gcgggctgaa tgagaattac ggccgcgagc tgatggagtt
     2521 gcacaccctc ggtgtggacg gcggttatac gcagaaagat gttacagaag tggcgcgctg
     2581 cttcaccggc tggacgatcg aaaagcccag gcagtacccg gagttcaagt tcgacgaccg
     2641 gctgcacgat cccgacccga aattggtgct ggggaaaaag attcacgcag gcgggatgaa
     2701 ggacggcgag caggtgatcg acctgctcgt gcatcacccg agtacagcga agtttatttc
     2761 cacgaaactg gcgcgccgtt ttgtttcgga caatccaccg ccggcgctgg tcaatcggat
     2821 ggcgcagacg tttcaatcga gcgacggcga tatccgcgcc gtgctgaaga ccatgatctg
     2881 gtcgccggaa ttctggtcgc gggaatcgta ccgcgcaaaa attaagacac cctacgagct
     2941 ggttgtttcc gcggtgcgcg ctctgggcac ggatgtgggc acgcccatgc cgctggtgca
     3001 gtgggtgggg cgaatcggag agccgctgta tcagtgccag ccgccgacag gttattcgga
     3061 caaagccgat acgtgggtga acaccggagc gctgttgaac cggctgaact actcgctcgc
     3121 gctcgccggc aacaagatgc gcggcgcgcg caccgatgtg acttcgctgc tgggcgtgga
     3181 accgtccgcg gatccaaggg cggcactgga ccgggcggtg caagtattcc tgggcgggca
     3241 ggcgggagcg actacggtgg agacgctgga gaaacaactc gataacccgc aagttttgca
     3301 ggccaagctc gatgatccgc gcaagcaggt ggatctcggt gtggttgccg gattggtgct
     3361 gggcgctccg gaatttcaaa ggcgatgacg tacgagaggc cgcgatgagc tacaaatgct
     3421 gtgagggcgt gagaggcttg aaattttcgc ggcgctattt tctgaagcag ggtggcgtgg
     3481 ccatggtcgg cctgagcgcg atgccggcgt ttttgcagcg cgccgtggct gccacgtcca
     3541 tgccgaac
//
LOCUS       DASYVO010000385         4542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_56085, whole genome shotgun sequence.
ACCESSION   DASYVO010000385 DASYVO010000000
VERSION     DASYVO010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56085"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VGR03_12940"
     CDS             <1..325
                     /locus_tag="VGR03_12940"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEV1995228.1"
                     /translation="ARLPRVEIKDPGKVIGTNTVTHMQAGLYYGAVDMVDGMLARMKK
                     ELGEDAKVVATGGEAKLVSKGSKHIQHTDEFLTLTGLRLIWEKNQPAEHGKSAAAAPA
                     KKAQS"
     gene            397..1119
                     /locus_tag="VGR03_12945"
     CDS             397..1119
                     /locus_tag="VGR03_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995229.1"
                     /translation="MDGWNYFNYFTEIEEYFWKKRGAHLLVSPLDWAIMEAWQKAGVP
                     LEAALKGIDKAFESYQRSRRGAGKPLKNLAYCTDAVLEAAEEQQEAAAGSTPKVVRAT
                     SSEAFSRDELKAYFAKNVAHLKRAAEKSLPQQPEIATRLKEIAESLESSSQILDAPGT
                     LDLEDLERRLTILDEKIHALLTSGAPEELMLKIRREVDGQLAMYRRKMKAEQLAMVEK
                     QYVQKRLLEELGVPRLSLFYLS"
     gene            1215..2522
                     /gene="rlmD"
                     /locus_tag="VGR03_12950"
     CDS             1215..2522
                     /gene="rlmD"
                     /locus_tag="VGR03_12950"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1995230.1"
                     /translation="MADELRLSIEKLIYGGDGLAHADGNTVFVPYVLPGEEVRANAKS
                     RKKKLVWAELLEVTSPAKEREKAKCAHFQKCGGCHYQHIPATEQLRLKKDILRETLSR
                     LGGVSWDGAIVEHSAEPYGYRNRAQWAVRSGMPRALGYFLPESSVIVPIDECPVLSPR
                     LAHTFGQLQEMARAGRLPEGIQEIEAFADSSDEKIALNVAFEKFPKPAAELAAAFREA
                     LPQIESLLLLDQKKNRFELTGPGYLTHEAGEFKFRVSHLSFFQVNRFLVEDLLKTVTA
                     NAHGAVALDLYSGVGFFTLPLAKTFQRVVSVDANLAATRDLYANAEIAGVTIVSHNEH
                     AEEFLKKTEEKPDFVVLDPPRAGLGAETAEKLAELGAKEIVYLSCDPSTMARDLAVLT
                     GSPRKPKEISGPSIRYEITEMHLFDLFPQTFHIETLVRLRRVP"
     gene            2519..>4542
                     /locus_tag="VGR03_12955"
     CDS             2519..>4542
                     /locus_tag="VGR03_12955"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEV1995231.1"
                     /translation="MRLPAVAIAAAFACGILLGLHPSVARNASSHILLSSSFIFIAVL
                     ILAGALLVRIRRIFLAAVASLLCWVSLGFLGVCIAEQPRDANHVISLVEQGRLPLKTP
                     LRWHGYLRDEPTRLPWGYGYEIELSGVEFEEALHPARGGLRLSFTAHPEGAPPPDLHA
                     GDEVAVLTEAKRPQVFRDEGAFDRRAYLAQQNIDLVATLRAPELIERISTSNPTIGTV
                     LARARRRLRDEIDELFAVTPQAAGVLRAMLLGDRNFVDRAEAVDFQKTGVFHVLVVAG
                     LHVGALAFAFYWVGRKLRLSRVWTMLFTLTMLSAYVAVVEQRAPVLRAAMMAAIVVLG
                     GFFFRRLELLNSAAVAALILIAARPLGLRDSSFQLTFVAIGCIAGLALPWLEKTVQPY
                     VRALRGWRDVTRDTAHEPRAIQFRIDLRSLAHWLAAQLPQRLGKPTGDALVAGLSLTF
                     RVWELLVITLALQTGMLPLMARDFHRIPLAAPLVNLAAVPLTGVVVPLGFLTLACGLI
                     LPAAGKILAAPLAWLTALLLHIVHWFAHFPRWSYRIPGPPFWLIVLFFAMAILLTAEM
                     RMNHPLRRTVVWGLCVAFIACALTIAIYPFGERWTKGRLELTVLDVGQGDSLFVVSPG
                     GKTLLIDGGGAFGGFPGHEQHNGVDPGEEAVSPYLWSRGFQKLDVVALTHA"
BASE COUNT          950 a         1193 c         1435 g          964 t
ORIGIN      
        1 cgcgcgtctg ccgcgggtgg agatcaagga ccccggcaaa gtaatcggga cgaacaccgt
       61 cacgcacatg caggcaggtt tgtactacgg cgcggtggac atggtggatg ggatgctcgc
      121 acgcatgaag aaagaacttg gcgaagatgc gaaggttgtt gcgaccggcg gcgaggcgaa
      181 gctggtctcc aaaggctcga agcacatcca acacaccgat gaatttctta cgctaacggg
      241 cttgcgattg atctgggaga aaaaccagcc agcggaacac ggcaagagcg cagcagcggc
      301 gccggcaaag aaggcgcaga gttaagcctc gggcagtggc gacgcagacg ggatcgaggc
      361 cggcccccgg acattcaacg gcgcatcacc aacgaaatgg acggctggaa ctacttcaat
      421 tactttacgg aaatcgaaga atacttctgg aagaaacgcg gggcgcatct gctggtatcg
      481 ccgctggact gggcgattat ggaggcgtgg cagaaagccg gggtgccgct ggaagccgct
      541 ctgaaaggga tcgacaaggc gtttgagagt tatcagcggt cgcggcgcgg tgcgggcaag
      601 ccgttgaaga atctggcgta ttgcacggac gcggtgctgg aggcagccga agaacagcag
      661 gaggcggctg cgggaagcac tccgaaagtt gtgcgcgcga cgtccagcga agctttttca
      721 cgagacgaac tgaaagcgta ttttgcgaag aatgtggcgc atttgaaacg tgccgcagaa
      781 aagagtttgc cgcaacagcc ggagatcgcc actcggttaa aggaaatcgc ggaatcgctg
      841 gagtcgagca gccagatcct cgatgcgccg gggacattgg acttggagga tttggagcgc
      901 cggctgacga ttctggatga gaagatacat gcgctgctga cgagcggtgc gccggaagaa
      961 ctcatgctta agatccggcg cgaagtggat gggcaactgg cgatgtacag gcggaagatg
     1021 aaagcagagc aactggcgat ggtcgagaaa cagtacgtgc agaaacgact gcttgaagag
     1081 ctcggagtgc cacggctgag tcttttctac ctatcgtagc gccagcgaac aaaatcgccc
     1141 ggcattcgaa aacaagacag ggcgacttta tttcacgcct acaccggcga attctcttag
     1201 aataggacat tcttatggct gatgagttaa gattatcgat tgagaagctc atttacggcg
     1261 gtgacggctt ggcgcacgcc gatggcaata ccgttttcgt gccttacgtt ctgcccgggg
     1321 aagaggtgcg cgcgaacgcg aaatcgagaa agaagaaatt ggtttgggcc gaactgctgg
     1381 aggtcacttc accggcgaaa gagcgcgaaa aagcaaagtg cgcccatttt cagaaatgcg
     1441 gcggctgcca ctaccagcac attccggcaa cggagcagtt gagactcaag aaagacattt
     1501 tgcgcgaaac gctttcgcgg ctcggcggcg tctcgtggga tggcgcaatc gtggaacatt
     1561 cagcggaacc gtatggctat cgaaaccgcg cgcaatgggc ggtgcgcagc gggatgcccc
     1621 gggcgctggg atacttcctg ccggaaagct cggtgatcgt gccgatcgat gagtgtcccg
     1681 tgctgtcgcc gcgcctcgcg cacaccttcg gccagcttca ggaaatggcg cgcgccggca
     1741 gattgccaga gggtattcag gaaatcgaag cctttgccga ctcgtcggac gagaagattg
     1801 ccctgaacgt ggcgtttgag aaatttccca agcctgcggc ggagctggca gctgctttcc
     1861 gggaggcttt gccccagatc gaaagtttgt tgctgctgga ccaaaaaaag aaccggtttg
     1921 aattgacagg gccgggttat ttgacgcacg aagccggcga attcaaattc cgagtcagtc
     1981 atctttcatt ctttcaagtg aaccgcttcc tggtcgagga tttgctgaag acggtgacgg
     2041 cgaacgcgca tggcgccgtg gcgctggatc tgtattcggg agtcgggttc ttcaccttgc
     2101 cgctggcgaa gacgttccaa agagtggtga gcgtagatgc caatcttgcc gcgacgcggg
     2161 atctctacgc caatgcggag attgcaggcg tcacgatcgt gtcgcacaac gaacacgcgg
     2221 aagagttttt gaaaaaaacc gaagaaaaac cagacttcgt ggtcctggat ccgccaaggg
     2281 caggattggg cgcggaaacc gcggagaagc tcgccgagct cggcgcgaaa gaaattgtgt
     2341 acctttcctg cgatccttcc acgatggctc gggatttggc ggtgttgacg ggctcaccac
     2401 ggaaaccgaa ggaaatctct gggccaagta tccgctatga aatcacggaa atgcatctgt
     2461 tcgacctctt cccgcagaca tttcacatcg aaacgctcgt gaggttgcga agagtgccat
     2521 gaggctgccg gccgtagcga tcgccgcggc gtttgcatgc ggcatcctgc tcggcctgca
     2581 tccttcggtt gcacgaaatg cttcttctca cattcttctt tcttcttctt tcatctttat
     2641 tgctgttctc attctggccg gagccctcct tgtaagaatc agacgcatct tccttgcggc
     2701 ggtcgcttct cttctctgct gggtatcgct tggattcctg ggagtttgca ttgcggaaca
     2761 gccgcgggat gccaaccacg tgatctcact cgtggagcaa ggacgactcc ctctgaagac
     2821 gccgctgcga tggcacggct atcttcgcga tgagccaacg cgcctgccat ggggctatgg
     2881 atacgaaatc gagctttccg gtgtcgagtt cgaagaagcg ctgcatccgg cacgcggtgg
     2941 gttgcgcctg agcttcacag cgcatccgga gggagcgcca ccgccggatc ttcatgcagg
     3001 agacgaagtg gcagtcttga ctgaagcaaa gcggccgcaa gttttcaggg atgaaggcgc
     3061 gttcgatcga cgcgcgtatc tagcgcaaca gaacatcgat ctggtggcca ctctgagagc
     3121 gccggagcta atcgaacgga tatccacgtc aaacccaacc atcggaaccg ttctcgcgcg
     3181 ggcacgaagg agattgcgcg acgagatcga tgagctgttc gcggtcacgc cgcaggccgc
     3241 cggggtgttg cgggcgatgc ttctcggtga ccggaatttt gtcgaccggg cggaagccgt
     3301 ggattttcag aagacaggag tttttcacgt actggttgtg gcaggtttgc acgtcggggc
     3361 gctcgctttc gcgttctact gggtgggtcg aaagctgcga ctgtcgcgag tgtggacgat
     3421 gctgttcacg ctcaccatgc tctccgctta cgtggccgtc gtcgagcagc gcgctcccgt
     3481 gctgcgggcc gcaatgatgg cggcgattgt tgtgcttggc gggtttttct ttcgccgatt
     3541 ggagctgcta aattctgcag cggtggcggc tctgattcta atcgcagcaa ggccgctcgg
     3601 cttgcgcgat tccagttttc aactgacttt cgtggccatc ggctgtatcg ctggtctggc
     3661 gctgccgtgg ctggaaaaga cggtgcagcc gtacgtgcgc gcgctgcgag gctggcgcga
     3721 cgtaacacgg gacaccgctc acgagccgcg cgcaatccaa ttcagaattg atctacgctc
     3781 gctggcgcat tggcttgctg cgcagctgcc ccaacggctg ggcaagccaa cgggcgacgc
     3841 tctagttgcc ggattgtctc tgacgtttcg tgtgtgggag cttctggtaa tcacactggc
     3901 gctgcagacc ggaatgctgc cgttgatggc ccgtgatttt catcgcatac cattggcggc
     3961 gccgctggtg aacctggcag ccgtgccgct gacgggtgtg gtcgtcccac ttggatttct
     4021 gaccttggca tgcgggttga ttctcccagc ggctggaaag atccttgcag ctccgcttgc
     4081 ctggctaacc gcactgctct tgcatattgt tcattggttc gcgcattttc cgcggtggag
     4141 ttaccggatc cccggtccac cgttctggtt gatcgtcttg ttttttgcca tggccattct
     4201 gctcaccgca gagatgcgca tgaaccatcc gctgcgaaga accgtcgtct ggggattgtg
     4261 cgtggccttc atcgcctgcg cgctgacgat agcgatctat ccctttggag agaggtggac
     4321 aaagggcagg cttgaattga cggttcttga tgtaggccaa ggcgattcgc tgtttgtcgt
     4381 ttctcctggt ggaaaaactc tattgatcga cggcggagga gcgttcggcg gttttcccgg
     4441 gcatgaacaa cacaatggcg tcgacccggg ggaagaagcc gtgtcgccgt atctatggtc
     4501 gcgaggattt cagaagttgg atgttgtcgc gctgacgcac gc
//
LOCUS       DASYVO010000386         5480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_80063, whole genome shotgun sequence.
ACCESSION   DASYVO010000386 DASYVO010000000
VERSION     DASYVO010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80063"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..2162)
                     /gene="glyS"
                     /locus_tag="VGR03_12960"
     CDS             complement(18..2162)
                     /gene="glyS"
                     /locus_tag="VGR03_12960"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV1995232.1"
                     /translation="MKASAKEKRLELLFEVGCEEIPAGMLPKAEEELRTNIDKLLTAE
                     NLFDGVSVETFSAPRRLTAWVRGLLAKQADVELEVTGPPKSVAYDSVGAPTRAALSFA
                     DKQRVHVNDLYLIQTPKGEYLAAKQIKLGRTAEQILIAVLPRAIHDLTWPRSMTWTGL
                     DGARFIRPIRWIVAVLDGKRLKLSVAGIAAGRTTRGHRFLGSSVIPVGNFLDYEKGLQ
                     RNGVIVRPGRRQEKIAAELEAHAKRGNYRIHEDASLRKLVAYLNEYPSVIQGDFDPAF
                     LSLPDEILVTVMRDHQKYFAVEKKNGELAPHFLAVINVDKDSKGLIRAGHERVLRARF
                     ADAQFFWQADQKCRLADYLPKLERVTYESRLGSYRDKVERIRAIARWFTEQWFNLGML
                     HAHVAEADRAAELAKCDLATEMVREFTELQGIVGGLYARAQGESDEIADAVYDHYRPV
                     GLEDPIPRNLTGCAVALADKLDSVVGCFAVGVVPTGSSDPYALRRAALGIVKIILEKK
                     LPVSLSLAIGSASKALLTHKPKRGVTPGQEGQILDFILDRAKFVFREREGFGYDEVSA
                     VFRAGADDLVDAQKRLVALKAIRKSKNFEPLAVSFKRIRKILEKANVAPGDARHVNPE
                     LFEGAAERELYSAVRDAASKVQTQKRAGKYQEALETIAGLRKAVDQFFDGVMVMAENE
                     AVRSNRLALLAELLREFTTVADFSEIGGDERR"
     gene            complement(2159..3103)
                     /locus_tag="VGR03_12965"
     CDS             complement(2159..3103)
                     /locus_tag="VGR03_12965"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV1995233.1"
                     /translation="MKSKVKLKKKPKGLNFQDLILQLSHFWVKQGCVLQQPYDVEVGA
                     GTMHPETFLRVLGPQAYRVGYVQPSRRPADGRYGENPNRLYKHSQFQVVLKPSPADVQ
                     DVYLKSLGAIGINLKEHDIRFEEDNWESPTLGAWGIGWQVLLDGLEITQFTYFQQSGG
                     IDLDPISVELTYGLERICAFLQGVESVYDLRWSADKTYGDIRLMEEQQFSEYSFDRSD
                     APSIRSEFELHEKQAKELLDGFKGAEGKGRVRYPLLAAYDHCLKCSHLFNLMDARGVI
                     STTERAALMARVRTLACRTAASYLSQIGGAAPQREVRA"
     gene            complement(3100..3840)
                     /gene="recO"
                     /locus_tag="VGR03_12970"
     CDS             complement(3100..3840)
                     /gene="recO"
                     /locus_tag="VGR03_12970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV1995234.1"
                     /translation="MPARETEAIILKTFPLGEADRLVSFFGRSSGRVRGVAAGARRVK
                     NRYGSTLEVLSHVQIWYVEKETRELVRIQQADLLESFYKAQSDYGLSTGLAVISEISE
                     LILPEREVSEPMFRLMLLATREVERTGDWTLPLSYFAFWTVRLGGWLPRFDRCVSCGT
                     PFGSEPAFYDAHKSGLFCEKCRRSGMRPLHVETRDLAERFAGERLDRIEYEKSMQPSA
                     RELREAGLAWIEHHVERRLMTRELLETT"
     gene            4194..4862
                     /locus_tag="VGR03_12975"
     CDS             4194..4862
                     /locus_tag="VGR03_12975"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV1995235.1"
                     /translation="MFIPLKDLNPSRTYPFVNLTLILANITAFIYTIGLEVTLPRRTF
                     EAFIFSYSTVPARFPAFLAGQASFEVSFVPLVTSMFLHAGFLHVAGNMLFLWIFGDNI
                     EDFFGHLPYLFFYLVCGIGAGLLHVLFNLYSNVPALGASGAISGVMGAYILLYPRARI
                     LTLVFIFPLPVPAVIFLGLWYVMQFLSGINALGGGPSGGVAVWAHIGGFLLGMLLTGM
                     VRRR"
     gene            complement(5007..>5480)
                     /locus_tag="VGR03_12980"
     CDS             complement(5007..>5480)
                     /locus_tag="VGR03_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEV1995236.1"
                     /translation="RVFKQVRQEYKDIDSRHLYIDTLAMELVRDPGQFDVIVTCNLFG
                     DIISDLGAQLAGGLGLAPSGNIHPGKTSLFEPVHGSAPNIAGKGIANPLGSVLTSSMM
                     LEFLGWKPEAAALRTAVRQALHENYVTTDLGGDKLTVEVGDWLAIRSVEVLAQQH"
BASE COUNT         1080 a         1554 c         1660 g         1186 t
ORIGIN      
        1 agtaacactt gagattatta acggcgctca tcaccgccaa tttcagaaaa atcggcgacc
       61 gtggtgaact cgcggagtaa ttccgcgagc aatgcaagac ggttgctacg aacggcctca
      121 ttttcggcca tcaccatgac cccgtcgaaa aattgatcga cggcttttcg aagaccggcg
      181 atggtctcca atgcttcctg atacttcccc gcgcgtttct gggtttgcac tttggaggcg
      241 gcgtcgcgaa ccgcggagta gagttcgcgt tccgcggcgc cctcgaacag ttcgggattc
      301 acgtggcgag catcgccagg agccacgtta gccttttcaa ggatcttgcg aatgcgcttg
      361 aatgaaaccg cgagcggctc gaagttcttt gatttacgaa tggccttcag cgccaccagg
      421 cgcttctgag cgtccaccag gtcgtcggca ccggcacgaa aaacggcgct gacctcatcg
      481 tagccgaagc cttcccgttc gcggaagaca aacttggcgc ggtcgaggat gaagtcgagg
      541 atttggcctt cctggcccgg cgtaacgccg cgcttgggtt tgtgagttag caacgccttt
      601 gacgcggagc caatagccag cgacagtgag acgggcagct tcttctccaa aatgatcttg
      661 acgatgccga gcgcggcgcg gcgcagtgca tagggatcgc tcgagccggt gggcacgact
      721 cccacggcaa aacagccaac gacggaatcg agtttgtccg caagcgcgac ggcgcagccg
      781 gtcaggttgc gcgggatggg atcttcgagt ccgacggggc gatagtgatc gtagacggcg
      841 tcggcaattt cgtcggattc gccctgggcg cgtgcgtaca ggcctccgac aatgccctga
      901 agctcggtga actcgcgaac catttcggtg gcgaggtcgc acttggcgag ttcagcagcg
      961 cggtcggctt ccgcgacgtg agcgtggagc atgccgaggt tgaaccactg ttcggtgaac
     1021 cagcgggcga tcgcgcggat tcgttcgacc ttgtcgcgat aactgccgag gcgcgattcg
     1081 tacgtgacgc gttccagttt tggcaaatag tcggcgagcc gacatttctg atccgcctgc
     1141 cagaaaaatt gcgcgtcggc gaagcgcgcg cgaaggacgc gttcgtggcc agcgcggatc
     1201 agccctttgg agtccttgtc aacattgatg accgcgagga agtgcggagc cagttcaccg
     1261 tttttctttt ccacggcgaa atacttctgg tggtcgcgca tgacggtcac caggatttcg
     1321 tccgggaggc ttagaaatgc cggatcgaaa tcgccctgga tgacgctcgg gtattcgttg
     1381 aggtaggcca ccagttttcg aagcgaggcg tcttcgtgga tgcggtagtt tccccgcttg
     1441 gcgtgggctt cgagttccgc cgcgattttc tcctggcggc ggccggggcg gacgatgacg
     1501 ccgttacgct ggagtccttt ctcgtagtcc aggaagttgc cgacgggaat gacgctcgaa
     1561 ccgaggaagc gatggccgcg agtggtgcga ccggcggcga tgccggcgac ggagagtttc
     1621 aaccgcttgc cgtcgagtac cgcgacgatc cagcggatgg gacgaatgaa tcgtgcacca
     1681 tcgaggccgg tccaagtcat ggagcgtggc caggtcagat cgtgaatggc gcgggggagg
     1741 accgcgatca gaatttgttc agcggttctg cccagcttga tctgtttagc ggccaggtac
     1801 tcgcccttgg gggtctgaat cagatagaga tcgttgacgt ggacgcgctg cttatcggcg
     1861 aagctcagtg ccgcgcgtgt cggcgcgccg acggagtcgt aggccacgga tttgggcgga
     1921 ccggttactt ccaactcaac gtcagcctgt ttcgcgagca gtccacggac ccaagcggtc
     1981 aggcggcgcg gcgcgctgaa tgtctctacc gatacgccgt caaatagatt ttcagcggtg
     2041 aggagtttat cgatgttggt gcggagttcc tcttcggctt tcggaagcat cccggcgggg
     2101 atctcttcgc agccaacttc gaaaaggagt tcgaggcgtt tctcttttgc ggaggccttc
     2161 atgcccgcac ctcacgctgc ggtgcggcgc cgccgatctg tgagaggtac gatgcggcgg
     2221 tgcgacacgc gagggtgcgc acccgggcca tgagcgcggc ccgctcggta gtggagatta
     2281 cgccgcgcgc gtccatcaaa ttgaataggt gcgaacactt gaggcaatgg tcgtaggcag
     2341 caagtagtgg ataacgaacg cggcccttcc cttccgcacc tttgaaaccg tcgaggagtt
     2401 ccttggcctg cttctcgtgc aattcgaatt cggagcgaat cgaaggcgca tcgctacgat
     2461 cgaagctata ttcggaaaac tgctgctctt ccatcaggcg gatgtcgccg taagtcttgt
     2521 ccgcggacca gcgaaggtcg tagacgctct ccacaccctg aagaaacgcg cagatgcgct
     2581 cgagaccgta ggtgagttcg acggagatag ggtcgaggtc gatgccgccg ctctgctgga
     2641 agtaagtgaa ctgtgtgatt tcgaggccat cgagcagaac ttgccagccg atgccccacg
     2701 cgccgagggt cggggattcc cagttgtctt cttcaaaacg gatgtcgtgc tccttaaggt
     2761 tgatgccgat ggcaccgagg cttttcaggt agacgtcctg cacgtcggcc ggagagggct
     2821 tgaggaccac ttgaaattgc gaatgcttat aaaggcgatt gggattctcg ccgtaacgac
     2881 cgtcggcggg ccgacgacta ggctgaacat atccgactcg ataggcctgc gggccgagca
     2941 cgcgcagaaa agtttccggg tgcatcgtgc cggcgccgac ttccacgtcg tagggctgct
     3001 gaaggacgca accttgcttc acccaaaagt gcgagagctg caagatcaga tcctggaaat
     3061 tgaggccctt cggttttttc ttaagtttta ctttagattt caagtggtct ccaaaagttc
     3121 gcgtgtcatc agccgtcgtt cgacgtggtg ctcgatccat gccagcccgg cctcgcgcaa
     3181 ttcccttgcg ctgggttgca tggacttctc gtactcgatg cgatccaacc tctcgccagc
     3241 gaaccgttca gcaagatctc gcgtctcgac gtgcaacggc ctcattcccg agcgccggca
     3301 cttctcgcaa aagaggccgg acttatgcgc gtcgtaaaaa gccggctccg agccaaacgg
     3361 cgtgccgcaa gaaacgcagc ggtcaaagcg cggcagccag ccgccgagcc ttaccgtcca
     3421 gaaagcgaag tagctcagcg gcaaggtcca atcgccggtg cgctcgactt cgcgcgtggc
     3481 cagcagcatc agccgaaaca tgggctcgga gacttcgcgc tccggcagaa tgagctccga
     3541 gatctcgctg attactgcga gcccggtgct gaggccgtaa tcgctctgtg ccttataaaa
     3601 ggattcgagc aaatcagctt gctggatgcg caccagttcg cgagtctcct tctcgacgta
     3661 ccaaatctgg acgtgagaga ggacctcgag cgtcgagccg taacggttct tcactcggcg
     3721 ggcgcccgcg gccacgccgc gtaccctccc cgaggagcgc ccaaaaaaac tcaccaggcg
     3781 gtccgcttcg cccaggggaa atgtcttcag aatgatggct tccgtttccc gtgcaggcat
     3841 gagcagtcgg cgcgccgacg ctgcccggac aaactgcagc actccgtccg aaacgcgcgc
     3901 acacgcagaa ctattgtaaa taccacacac gcactcatcg cgcaatgttc ccgtagaaaa
     3961 ttccccggca aatcaagccg agagttgcgg cgaaattgaa aactgcactc tttgaataca
     4021 ggggatcttt tcacgcgaga cgagataagg gcagcagatt gaggcgattg caggtgcaaa
     4081 tttattctgt taactagcgg acttttcgca tttacattat cggacgctgg aagccgcttc
     4141 tccgcccttt tcatttcgcg tgtgtttcgc tactctgctg cggaccttct cccatgttca
     4201 ttcccctcaa ggacctcaac ccatcgcgca cttatccttt cgtcaatctc acgctgatcc
     4261 tcgccaacat cacggcgttt atctacacga ttggcctcga ggttacgctc ccacgccgga
     4321 cctttgaagc cttcatcttc tcgtactcca cggtgcctgc tcgcttcccc gcattcctgg
     4381 caggacaggc gagctttgag gtatctttcg tgcctctggt taccagcatg tttctgcatg
     4441 ccgggttcct gcacgtcgcc gggaacatgc tttttctctg gatcttcggg gacaatatcg
     4501 aggatttttt cgggcacttg ccgtatctct tcttttatct ggtctgtggg atcggagcgg
     4561 ggctactgca cgtgctattc aatctgtact ccaacgtgcc tgccctcggc gcgagcggag
     4621 ccatctccgg cgtgatggga gcttacatac tgctctatcc ccgggcacgc attcttacac
     4681 tcgtgtttat cttccctctg cccgtgcctg ccgtgatttt tcttggcttg tggtacgtca
     4741 tgcagtttct ttcggggatc aacgcgctcg ggggcggtcc ttccggagga gtggccgtgt
     4801 gggcgcatat cggagggttc ctgttgggga tgctactgac cgggatggtg cggcgaaggt
     4861 aggaggtaaa tcgagatttt cggatattaa agtattactt tagattgagg ggtgcaattt
     4921 aaccaggaac tacatcgcaa aagtggcagc tgggccccca ccggagccgc ggtaaactgt
     4981 cgcactcgaa aaggcggcgc agtcacttag tgttgctggg cgaggacttc gacggatcta
     5041 atggcgagcc aatcgccgac ctcgacagtg agtttgtcgc cgccgagatc cgtcgtcaca
     5101 tagttctcgt gcaaagcctg tcgtaccgca gtgcggagcg cggcagcctc aggcttccag
     5161 ccgaggaatt ccagcatcat cgacgaagtg aggacggaac ccaacgggtt ggcgatgccc
     5221 tttccagcga tgttgggggc ggagccatgc accggctcga aaagtgaagt cttgcccggg
     5281 tgaatgtttc cggacggggc gaggccgagg ccgcctgcaa gctgcgcgcc caggtcgctg
     5341 atgatgtcac cgaaaagatt gcaggtgacg atgacgtcga attggccagg gtcgcggacg
     5401 agctccatcg ccagggtgtc gatatacagg tggcgggaat cgatgtcctt gtattcctgg
     5461 cggacttgct taaaaaccct
//
LOCUS       DASYVO010000387         2838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_249304, whole genome shotgun sequence.
ACCESSION   DASYVO010000387 DASYVO010000000
VERSION     DASYVO010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249304"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VGR03_12985"
     CDS             <1..484
                     /locus_tag="VGR03_12985"
                     /inference="COORDINATES: protein motif:HMM:NF012611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995237.1"
                     /translation="PSGAGHFVKMVHNGIEYGLMAAYAEGLNILKHANAGKSHREADA
                     ETTPLRNPEHYQYDFNLADITEVWRRGSVVASWLLDLTAISLLDQPTLERFSGRVSDS
                     GEGRWTILAAIESTAPAPVLTASLFQRFTSRGEDDFAAKVLSAMRFQFGGHIEKKSGH
                     "
     gene            556..1314
                     /gene="pgl"
                     /locus_tag="VGR03_12990"
     CDS             556..1314
                     /gene="pgl"
                     /locus_tag="VGR03_12990"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEV1995238.1"
                     /translation="MDREIRILADGAAIAKHAAQEFVQAAAAAVLSKGSFIVALAGGS
                     TPKALYGLLVNDPSLRSQVPWDKIHLFFGDERHVAPDHPDSNFRMASEAMISKSPLKL
                     EQVTRIKGEYPDADHAALEYEKALREYFKLKDGEYPRFDLVLAGMGNEGHTLSLFPGT
                     KALHADGRMVVRNWIGKLYTERITLTAPAASNAAQVIFMVTGADKAPALKAVLEGPFE
                     PEQLPAQLLQPQNGKLLWLVDTAAGSMLSIGIRE"
     gene            1385..2464
                     /locus_tag="VGR03_12995"
     CDS             1385..2464
                     /locus_tag="VGR03_12995"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEV1995239.1"
                     /translation="MATSTTQGKIETYLGAKADSLLGFKSPKIAKERLHLPGPDFVDR
                     IYALTDRNLRVLANLQRMFGHGRLSGTGYLSILPVDQGIEHSGGASFAKNPDYFDGGN
                     IVKLAIEGGCNAVASTFGVLGSVARKYAHKIPFIVKLNHNELLTFPNRADQIFFGTVQ
                     QAYDMGAAAVGATIYFGSDQSGRQIVEVSQAFAHAHQLGMATVLWCYLRNNAFKKDKD
                     FHVSADLSGQANHLGVTIQADIIKQKLPENNGGYAALNMGDSSYGKFDKRIYTDLCSD
                     HPIDLTRYQVANCYMGRAGLINSGGASGANDFEDAVATAVINKRAGGTGLISGRKAFQ
                     RPMKEGVQLLNTIQDVYLSPEVTIA"
     gene            complement(2610..>2838)
                     /locus_tag="VGR03_13000"
     CDS             complement(2610..>2838)
                     /locus_tag="VGR03_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995240.1"
                     /translation="AMQYSVKKFLMALTLGRIVRYSLLAFLGARYGRQVLTVISQHGH
                     PVLIAVIGLITAAMAALIFILVSKRKKRAHG"
BASE COUNT          628 a          910 c          768 g          532 t
ORIGIN      
        1 cccttccggc gccggccact tcgtcaaaat ggtccacaac ggcatcgagt atggcttgat
       61 ggctgcttac gccgagggct taaacattct caagcatgcc aacgcgggca aatcccaccg
      121 cgaggccgac gccgaaacca ctccgctgcg aaatcccgaa cactatcaat acgatttcaa
      181 tctcgccgac atcacggaag tgtggcgccg cggcagcgtc gtcgcatcat ggctgctcga
      241 tctgaccgcc atttccctgc tcgatcagcc cactctcgag cgcttctccg gccgcgtctc
      301 tgattccggc gaaggccgct ggaccatcct cgccgccatc gaatccaccg cacccgcgcc
      361 cgttctgacc gcgtcactgt ttcagaggtt cacatcacgc ggcgaagatg acttcgcggc
      421 aaaagtattg tccgcgatgc ggttccagtt tggcgggcac atcgaaaaga agtccgggca
      481 ctagcgattc cagaagccgt ttgccgcact gccgcgcagg acagaacggg aatcatcacc
      541 ggagggagag cgcgagtgga tcgtgaaatc cgaatcctgg cggacggcgc agccatcgcc
      601 aagcacgcgg cgcaggagtt cgtgcaagcc gcggcagcgg cggtactctc gaaaggctcc
      661 ttcatcgtgg cactggctgg gggatcgacc ccgaaagcac tctacggcct cctggtgaac
      721 gatccatcgc tgcgttcgca ggttccttgg gataaaatac atctgttctt cggcgacgag
      781 cgccatgtcg cgccggacca tccggacagc aattttcgaa tggcctcgga agcgatgatt
      841 tcgaaatcgc cgttgaagct cgagcaggtc acgcggatca aaggcgaata tcccgatgcg
      901 gaccatgccg cgctcgaata cgaaaaggct ctgcgcgaat atttcaagct gaaggatgga
      961 gagtatccgc gctttgatct cgtgctggca ggaatgggca acgagggcca cacgctttcg
     1021 ctattcccag gaacgaaggc gctgcatgcc gatggacgca tggttgtgcg aaactggatc
     1081 ggcaagctgt acaccgagcg catcacgctt accgcgccgg ctgccagcaa cgccgcgcaa
     1141 gtcatcttta tggtgacggg cgcggacaag gctccggccc tgaaagcggt actggaaggt
     1201 ccgtttgagc cagagcaatt gcccgcgcaa ctgctccagc cgcaaaatgg aaaattgctt
     1261 tggctggtgg acacggccgc cggcagcatg ctgtcgatag gaattcgaga atgaatagtt
     1321 gactgtttta aggtcacgct agatttgacc gcttagcggt cacacctgaa acgaggagaa
     1381 agaaatggcc accagcacca cccagggaaa aattgaaact tatctcggcg caaaggcgga
     1441 ttccctgctg ggattcaagt ctccgaagat cgccaaggaa cgcctgcatc tccccggccc
     1501 ggactttgtc gaccgcatct acgccttgac cgaccgcaac cttcgcgtgc tcgccaatct
     1561 ccagcgcatg ttcggccatg gccgcctcag cggtacgggt tatctctcca tcctgcccgt
     1621 cgaccagggc atcgagcact ccggcggcgc aagcttcgcc aagaatcccg actactttga
     1681 tggcggcaac atcgtcaagc tggccatcga gggcggctgc aacgccgtgg cttccacctt
     1741 cggcgtgctc ggctccgtcg cgcgcaagta cgctcacaaa atccccttta tcgttaagct
     1801 caatcacaac gagctgctga ctttcccgaa tagagccgat caaattttct tcggcaccgt
     1861 gcagcaagcc tacgacatgg gcgctgccgc cgtcggcgct accatttatt tcggctctga
     1921 ccagagcggc cgccagatcg tcgaagtcag ccaagccttc gcgcacgcgc accaactcgg
     1981 catggccacc gtcctctggt gctatctgcg caacaacgcc ttcaagaagg acaaggactt
     2041 tcacgtctcc gccgacctca gcggccaggc caaccatctc ggcgtaacta tccaggccga
     2101 catcatcaag cagaaactcc ccgaaaataa cggcggctat gccgcgctca acatgggcga
     2161 ttccagctac ggaaaatttg acaagcgcat ctacaccgac ctttgcagcg accaccccat
     2221 cgacctgacg cgctatcagg tcgccaactg ctacatgggc cgcgccggct tgatcaacag
     2281 cggcggcgct tccggcgcaa acgacttcga ggatgccgtc gccaccgccg tcatcaacaa
     2341 gcgcgccggc ggcaccggcc tcatctccgg ccgcaaagcc ttccagcgcc ccatgaaaga
     2401 aggcgtccaa ctcctcaaca ccatccagga tgtctacctg agccccgaag tcaccatcgc
     2461 ttaaagcgca gctctggcct tgcaagggca cgatacacct atcccgacca ggtcgggata
     2521 ggtgtatcgt ccccattcct atagaaggca cttccccggc gccgtaggcg cgacacatgt
     2581 cctgttgctc tcagtagctc agaagtcggc taaccgtgtg ctcgtttttt tcgtttgctt
     2641 acgagaatga agatgagagc cgccatcgcc gccgtaatca gcccgataac cgcaatgagg
     2701 accggatgcc catgttgcga gatgaccgtg agcacttgcc gtccatagcg agcacccagg
     2761 aacgccagga gagagtatct gacaattcgg ccgagcgtca gcgccatcag gaattttttc
     2821 acggaatatt gcattgcc
//
LOCUS       DASYVO010000388         7732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_25624, whole genome shotgun sequence.
ACCESSION   DASYVO010000388 DASYVO010000000
VERSION     DASYVO010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25624"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(391..699)
                     /locus_tag="VGR03_13005"
     CDS             complement(391..699)
                     /locus_tag="VGR03_13005"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEV1995241.1"
                     /translation="MEYHDRVLKCSECSAEFVFTAGEQMFFADKGFKNEPKRCKACKA
                     KRAEGGGGGGGHASPMRSETKTNCSQCGKETTVPFRPTQGRPVFCRECFQQRRAMGAA
                     "
     gene            complement(946..1143)
                     /gene="rpsU"
                     /locus_tag="VGR03_13010"
     CDS             complement(946..1143)
                     /gene="rpsU"
                     /locus_tag="VGR03_13010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV1995242.1"
                     /translation="MAEVIIQESESLENALRRFKRKVQQEDIIKEIKKHSFYMKPGEK
                     RRAKEALSRKRLRKKQRREAD"
     gene            1575..2813
                     /gene="glgC"
                     /locus_tag="VGR03_13015"
     CDS             1575..2813
                     /gene="glgC"
                     /locus_tag="VGR03_13015"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV1995243.1"
                     /translation="MSDMRDAIVVLLAGGQGERLWPLTRDRAKPAVPFGALYRIIDIT
                     LSNCINSNLRRIFILTQYKALSLNRHIRAGWSPLAGLGDYIEVLPPQMRVSQQWYQGT
                     ADAVYQNIYSIGSERSKFVFILSGDHIYKMNYSRMLQQHCDSGADVTVGTIEIPAARA
                     AKQFGVIETDKDWRIIGFEEKPAVPKESPHHAGHCNASMGIYIFNTQLLIPILLADSE
                     DPKSSHDFGKDVLPRIISKHRVFAYNFVDENAKDALYWRDVGTLDAYYEANMDLVSVS
                     PVFNLYDTSWPLRTWQHQYPPAKFVFADPERMGVALDSIVAGGSVISGGRVQKSVIGN
                     NVRVNSYCEVSDSIIYNHVNIGRHSRIRRAIIDRHVSLPERTEIGYDLEADRRRFHVS
                     DSGIVVVVRQESLIEEPESA"
     gene            complement(2863..3225)
                     /locus_tag="VGR03_13020"
     CDS             complement(2863..3225)
                     /locus_tag="VGR03_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995244.1"
                     /translation="MFFGPVAGGLVCAINFKRMGQEKKARLTIICSLLFTLLFVALID
                     FVLPDNEPLIRGIGLGVLVAGFVTFSNLQENDFETWSNQNQESKPANGWSSIGWGLLG
                     LLVLLVIVTVLAMFTPSV"
     gene            3480..4796
                     /gene="eno"
                     /locus_tag="VGR03_13025"
     CDS             3480..4796
                     /gene="eno"
                     /locus_tag="VGR03_13025"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV1995245.1"
                     /translation="MSTKIARVHARQVIDSRGNPTVEADVLLDGGALGRAAVPSGAST
                     GEHEALELRDGDKSKFLGKGVLKAVSNANTELAKSIAGLDAADQRALDRKMISLDGTP
                     TKSRLGANAILAVSMAVARAAAIALKQPLYKYLSRYSTDSSANLLPCPMMNILNGGAH
                     ADSSVDFQEFMVMPVGAPSFSEALRWGVEVFHALKTALKKHGYSAAVGDEGGFAPSCK
                     SNEEAIQIVLEAITAAGYKPGEQVSIALDPASSEFYDKASGKYVFKKSDKSAHSPAEM
                     VEYWAKWIEKYPIVSLEDGMGEEDWPGWKQLTQKVGSKSSSKRIQLVGDDIFVTNTEI
                     FSRGIKEGIANAILIKLNQIGTVTETIDAIELARKAGYNSIISHRSGETEDTFIADLA
                     IATAAGQIKTGSASRTDRIAKYNQLLRIEEELGSAARFHGRSALGN"
     gene            4922..5668
                     /locus_tag="VGR03_13030"
     CDS             4922..5668
                     /locus_tag="VGR03_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995246.1"
                     /translation="MTTPVTFVRFALAFWLSFPPGSPATAPWDKPPEQWDLAETFRVL
                     RDSPWSPAGTKLAAAWTQRHTDPQSGIVSNSPVNPEDTGMVRGIEINRNRPLPAVSLL
                     WWSSKTVRLARQRQRQLRHSGGAATVLAAEDLPDYVLAVEGSEPLRIFQDAREDLHDT
                     VFLELEDGLTLDLKSVLFFEGTADEDARAEFHFPRLSDGRPSLDPNSERIVFHCKASA
                     KTARLGRENAISLRAEFQPRTMRARGLPDL"
     gene            complement(6213..6512)
                     /locus_tag="VGR03_13035"
     CDS             complement(6213..6512)
                     /locus_tag="VGR03_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995247.1"
                     /translation="MATRKMTFTLPEDLAEQFVRRVPARERSKYLAAALNEKLSARDR
                     KLVEACRIANNDTEVRAIEKEFDAITEEAGESWSASTPRRSLVGKTRSHSRRRNS"
     gene            complement(6618..7304)
                     /locus_tag="VGR03_13040"
     CDS             complement(6618..7304)
                     /locus_tag="VGR03_13040"
                     /inference="COORDINATES: protein motif:HMM:NF018626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HEV1995248.1"
                     /translation="MKQKRSFTLRAALSAALAIASAASTAQVKPAETTARQEFLKKLV
                     AAAVERAHHAVRYDPAYVRIPYPGGDVPADTGVCTDEVIRAYRAVGVDLQKEAHEDME
                     QNFSSYPRKWRWLSGHPDANIDHRRVPNLMVFFGRRGETLAIGRRSEDYAPGELVTWD
                     LGGGVPHIGIVVGQKSAVSGRYMILHNIGRGPKIEDVLFSWKITGHYRYFGPPSPPEN
                     ASPSDGPPVR"
BASE COUNT         1569 a         2500 c         1972 g         1691 t
ORIGIN      
        1 tgatctggaa ttcttcttca acgccgatgg tgaagcggtg agccatggag tggccctccg
       61 ggtctcttgg tcttgttggg aactccgagc ttcgtatcat accctgccga ggctcaagaa
      121 tgcacctctc cgtctggtgg cggcgctaac ctcgattcag atgtctcgct gccaggaggt
      181 gtctaccagg agacgaggca caccgatgca aaaaatgtcg gaagccttcc ccaattccgt
      241 gcctatctgg attgtcacta caagatggaa tctacttgcg gtagacggca caaaaaaagt
      301 gggacggata ggccaatccg tcccacgcga agtattccgc cgttcgaact attctttcag
      361 acgtctcgac aactaattcc aattcctgct ttacgccgcg cccatggccc tgcgctgctg
      421 gaagcattcc cggcaaaaca ccggccgccc ttgtgtggga cggaaaggaa ctgtggtttc
      481 cttcccgcac tgcgagcaat ttgtcttcgt ttccgaacgc atcggtgagg cgtgaccccc
      541 gccgccaccg ccgccctcgg ctcgtttcgc tttgcaagct ttgcagcgct tgggttcgtt
      601 tttgaagccc ttgtcggcga aaaacatctg ttcgccggcc gtgaaaacga actcggcact
      661 acactccgaa cacttcagca ccctgtcgtg atattccatg agccgctctc ccctggtccc
      721 gtgtcgggca ggagaatggg cagactttgt acctgagagc cgagatgctt gaggaaacct
      781 gtatccgggg acgccgggcg cggatccctt gggcttgcgg gcacgaagct gaaacacgaa
      841 cccaaaactt ccctgcctta cgggggcctg attctcggaa gtgcggcgcc gagccgcgac
      901 gaaaacggat gggcggcccc gacccagtcg gaaccgccca ctcaattaat ccgcttcccg
      961 ccgctgcttc ttgcgcagtc gcttccgcga aagcgcttcc ttggcccggc gcttctcgcc
     1021 gggtttcatg tagaaactat gtttcttgat ttccttgatg atgtcttcct gttgcacctt
     1081 gcgcttaaaa cgccgcaggg cattttccag ggattcactc tcttggatga tgacttcagc
     1141 cactttatta attcaccccc aatccggttc ggattggtat cttgcggtgc ctctcgccgg
     1201 ccaagctacg accggctgtg tcctgctgcg ggcaccgccc ccggtactga ttatcgcatg
     1261 tattggtcgc ttcgcgggtg caccctgaaa ggtcgctggc ctcttctaat caattcctgc
     1321 catgcaagtc aaggaatctt tgcgttttct gaccggcaat cgcggtactg aacccacctc
     1381 ataactatcc gggtactccg ttgacgcaag tgtaacctgg gaacatttcc gtggatttgc
     1441 accgcgatac tcccccacga gccttcttcg ctcatcgatt accccttccc gccttcgcag
     1501 cggcctcgct tgtcttttcc cctgcttctc ttgttagaat gagccgcttc tctattcgcg
     1561 cccgggggca accaatgtcc gatatgcggg atgccatcgt agtactcctt gccggaggcc
     1621 agggcgaacg cctttggccg ctcactcgcg atcgcgccaa gccggccgtt cctttcggcg
     1681 ctctctatcg catcatcgac atcaccctct cgaactgcat caattcgaat ctccgccgca
     1741 ttttcatcct cactcagtac aaggccctca gcttgaaccg ccacatccgc gcgggctggt
     1801 cgcccctcgc cggccttggc gactacatcg aggtcctccc gccgcagatg cgcgtctcgc
     1861 agcagtggta tcagggcacc gccgacgcgg tctaccagaa tatctactcc atcggcagcg
     1921 agcgctcgaa attcgtcttc atcctttccg gcgaccacat ctacaagatg aattacagcc
     1981 gcatgctcca gcagcactgc gactccgggg ccgacgtcac ggtgggcacc attgaaatcc
     2041 ccgccgcacg ggctgcgaag cagttcggcg tcattgagac cgacaaagac tggcgcatca
     2101 tcggcttcga ggaaaagccc gccgtcccga aggagtcgcc gcatcatgcg ggccactgca
     2161 atgcctcgat gggaatctac atcttcaaca cgcaactcct gattcccatc cttctcgcgg
     2221 actccgagga tcccaaatcc agccacgatt ttggcaagga cgttcttcca cgcatcattt
     2281 ccaagcaccg cgtcttcgct tacaacttcg tcgatgaaaa cgcgaaagac gctctctact
     2341 ggcgcgatgt cggcacgctc gacgcctact acgaagccaa catggatctc gtcagcgtgt
     2401 ccccggtttt caacctatac gacacctcct ggccgctccg cacctggcag caccagtatc
     2461 cccctgcaaa atttgtcttc gccgatccgg agcgcatggg cgtcgcgctc gattccattg
     2521 tggccggcgg ctcagtcatc tccggcggac gcgttcaaaa atccgtcatt ggcaacaacg
     2581 ttcgcgtcaa cagttactgc gaagtctcag attccatcat ctacaatcac gtgaatatcg
     2641 gccgccactc gcgcatccgc cgcgccatca ttgaccgcca cgtcagcctt cccgagcgca
     2701 ccgagatcgg ctacgacctc gaagccgacc gccgccgctt ccacgtcagc gattccggca
     2761 tcgtcgtcgt cgtccgccag gaatccctga tcgaagagcc cgaatcggcc tagtaacaca
     2821 catcttgttt tgctctttta tcccagcagg ggatgggtcc tgctatacgc tcggtgtaaa
     2881 catggcgagg accgtcacaa tcacgagcag gaccagtaag ccgagaagtc cccaaccaat
     2941 agaactccac ccgtttgctg gtttgctctc ttggttctga ttgctccagg tctcgaagtc
     3001 gttttcctga agattggaaa aggtgacaaa acccgccacc agaactccaa ggccaatacc
     3061 tcggatcagt ggctcgttgt caggtaggac gaaatcaatc agagcgacga atagcaaggt
     3121 gaatagcaaa ctgcagatta ttgtcagccg ggccttcttc tcctgaccca ttcgtttgaa
     3181 gttgatcgcg caaacaagtc ctccggccac tggcccaaag aacatcgcag atatcccggc
     3241 gactctgggc cgccaaggcg acctctttgg ttctacgggt agagcttcaa tcggccgcgt
     3301 ggtggacgcg ttgcgctcga cacccaaatt ctcctctggc atgcctcgat cctccaagca
     3361 atgcgctacc gaggcggcca atctaccacc tcgatctact ttttctccgt gcctctgtgc
     3421 ctccgcggca ttcttctgta aaatcatcaa tccgcttcta ctcaggagaa cagaccacca
     3481 tgtccactaa gatcgccaga gttcacgccc gccaggtcat cgattcccgc ggcaatccca
     3541 ccgtcgaagc cgacgtcctc ctcgatggcg gagcgcttgg ccgcgccgcg gttccctccg
     3601 gcgcttccac cggcgagcac gaagccctcg agctccgcga tggtgacaaa tcgaaatttc
     3661 tgggtaaagg cgtgctcaag gccgtctcca acgccaacac cgaacttgcc aaatccatcg
     3721 ccggtctcga cgccgccgac cagcgcgccc tcgatcgcaa aatgatttcg ctcgacggca
     3781 cgcccaccaa gtctcgcctc ggcgccaacg ccattctcgc cgtctcgatg gccgtcgcac
     3841 gcgccgccgc catcgccctc aagcagccac tctacaaata tctttcgcgc tattccacgg
     3901 attcctccgc gaatttgctc ccctgcccga tgatgaacat tctcaatggc ggcgcgcatg
     3961 ccgacagttc cgtggacttc caggaattca tggtcatgcc tgtgggcgcc ccgagctttt
     4021 cggaagcgct gcgctgggga gtggaagttt tccatgcttt gaaaaccgcc ctgaaaaaac
     4081 atggctactc ggcggccgtt ggcgacgaag gcggctttgc ccccagctgc aaatccaacg
     4141 aagaagccat tcagatcgtg ttggaagcta tcactgctgc cggatacaaa cctggcgagc
     4201 aggtcagcat cgcgctcgac cccgcctcca gcgagttcta cgacaaggcc agcggcaaat
     4261 atgttttcaa aaagtccgac aagtccgcgc actctcctgc ggagatggtc gaatactggg
     4321 ccaagtggat cgaaaaatat ccgattgttt cgcttgagga tggcatgggc gaagaagatt
     4381 ggccaggctg gaagcagctt acgcagaagg tcgggtcaaa atcttcatca aaacgaattc
     4441 agctcgtcgg cgatgacatc ttcgttacca acaccgaaat cttttcccgc ggcatcaagg
     4501 aaggcatcgc caacgccatc ctcatcaagc tcaatcaaat cggcaccgtg accgaaacca
     4561 tcgacgccat cgagctggcc cgcaaagcgg gctacaactc catcatctct catcgttccg
     4621 gcgaaaccga agacaccttc atcgccgatc tcgccatcgc caccgccgcc ggccagatca
     4681 agaccggctc cgccagccgc accgatcgca tcgccaagta caaccagctt cttcgcatcg
     4741 aagaggaact gggctccgcc gcccgcttcc atggtagatc ggctctggga aattaacgtg
     4801 aggccggaac catggcgccg ttgtttgttc tcgagagcct ttccgcgcct cttgctcgtg
     4861 gggcgcctcg ctcttgctgt gcaactttcc cctgggccgc tgcatcgcat tcgctaaccg
     4921 tatgaccact ccagtcactt tcgttcgctt cgccctggct ttttggctct cctttccgcc
     4981 gggttcgcct gccacggctc cctgggacaa gcctcccgag caatgggatt tggcggagac
     5041 atttcgggtt cttcgcgact ctccgtggag tccggccgga actaaactcg ccgccgcctg
     5101 gacccagcgc cataccgatc cgcaatccgg aattgtctcg aattcgccgg tcaatccaga
     5161 ggacacgggc atggttcgcg ggatcgagat caatcggaac cggccgctcc ctgcggtttc
     5221 ccttttgtgg tggtcatcca aaaccgtacg tctggctcgc cagcgccagc gtcaattgcg
     5281 ccattccggc ggagccgcca cggttcttgc tgcggaagac ttgccggatt acgttcttgc
     5341 ggtggaagga agcgaaccac tccgtatttt tcaggatgcg cgcgaagact tgcacgacac
     5401 cgtcttcctc gagctcgagg atggccttac gctcgatctc aaaagcgtgc tttttttcga
     5461 gggcacggcg gacgaagacg cccgcgcgga gttccatttt cccaggctga gcgatggccg
     5521 gccttcgctc gatccaaatt ccgagcgcat cgtttttcac tgcaaagctt ccgcaaagac
     5581 cgctcgcctc ggacgtgaga acgcgatttc tcttcgcgcc gagttccagc cgcgcaccat
     5641 gcgcgcccgc ggcctcccgg atctctagcc cgtcatgctc ccaacgtgcg gccagtacag
     5701 tgaatttcct ttgtaggggc gcagcaccgc tgcgccccac tcgccgagcc gtcggctgca
     5761 aaatgccatt gcaggccatt ccaagagtga tcagctttca agggtgttgc ttctatctct
     5821 gacacttcat tggcaagaaa tacggccagt tcagcgccgc cactgcgtcg actggtcctc
     5881 gccaactgga atcgaaatgg aaatgaattc ttggaggtcg tggctaaaga tttcgtggtg
     5941 acatgagctg caactacaac tccaggatcg cgcgtagtcc gtcttctacg gccctcaatt
     6001 gttgtgagct gacactctca attctccgga ggaacctctc ctttgtcacc gcgcgtatct
     6061 ggtccatgac agcaaccgct gcccggcctt gacacgttag cgccaccaaa agcggaggac
     6121 tgccagcggg agagcttgag agagggatca ccaccatggt ccggcgatgc tggttcacga
     6181 tattcgtcgt cagcaccagg cagggccgcg tcttacgaat ttcggcgccg actgtgggat
     6241 cgagtcttac ccaccaaact tcgccgcggc gtggatgcgc tccacgattc cccggcttcc
     6301 tcggtgatgg cgtcgaactc tttttcaatg gcgcgaacct ccgtatcgtt gtttgcgatt
     6361 cggcacgcct ccaccaattt ccggtcgcgg gcggagagtt tttcgttgag cgcggcagcc
     6421 agatacttgg aacgctctct cgcaggaacc cggcgcacaa attgctcggc cagatcttcc
     6481 ggcagcgtga aggtcatctt tcgggtggcc atactcccat actaccatat aagtatgcga
     6541 cttgatgccc cccgagccag aaaaacttcg aaccgaagcc atagtcggcc taaactcact
     6601 tctcggcaaa atccgctcta tcgaactggc ggaccgtctg aaggagacgc gttctccggt
     6661 ggcgatggcg gcccgaaata acggtagtgc cccgtaattt tccagctgaa cagcacgtct
     6721 tcaatcttcg gccctcgccc gatgttgtgc agaatcatgt agcgtccgct tactgccgat
     6781 ttctggccca ccacaattcc gatgtgcggt acgcccccac ccagatccca agtgaccagc
     6841 tcgcccggtg cgtaatcctc tgagcgccgc ccaatcgcca gcgtctctcc tctacggccg
     6901 aaaaagacca ttaagttcgg cacgcggcga tgatcgatgt tcgcgtccgg atgccccgaa
     6961 agccatctcc acttgcgggg atacgatgaa aaattctgct ccatgtcctc gtgcgcttct
     7021 ttctgcagat cgacgcccac cgcgcgatac gctcgaatga cttcatcggt gcaaacccct
     7081 gtgtccgccg gaacatcgcc tccggggtat ggaattcgca cataagcggg gtcatagcgc
     7141 accgcgtgat gcgctctctc caccgcggct gcgaccagct tcttcaggaa ctcctgccgg
     7201 gccgtagtct ctgctggttt cacttgtgct gtcgatgcgg cggacgcgat cgcaagggct
     7261 gcggacagtg cagcgcgcag cgtgaacgat cttttttgtt tcataacgct tcccattcgc
     7321 cgaattctaa cagcaatttc caggcgccac gcagtggaat ggaatccgtt cagcactcta
     7381 acaggcagct gaaaaaggca aaattgtcat tcggaggaca tccgacgagg atgcccgaag
     7441 aatctcaact tcaaatgctt cagtcacata acgtttgggt gcttttcacg agcaactgca
     7501 aatcgctttt tagcaacctg ctgagaacga cgctctcaag ctggaaatca tccaggtaca
     7561 ggttagaatg ccgatgccag tatgagtgac gccgccaaag cgacttctca agaggatacc
     7621 ttgcgtttcc gcgtggcgtc ccttgcggac gccgaatccc tggcccgcct gatcaacgcc
     7681 gctttccgcg tcgagcagcc cttcatcgaa ggcgaccgca ttaaccctta tg
//
LOCUS       DASYVO010000389        21310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_233554, whole genome shotgun sequence.
ACCESSION   DASYVO010000389 DASYVO010000000
VERSION     DASYVO010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 21310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21310
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233554"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1384
                     /locus_tag="VGR03_13045"
     CDS             110..1384
                     /locus_tag="VGR03_13045"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV1995249.1"
                     /translation="MKAEIIAVGSELLTPDRMDTNSLFLTEELNKLGIEVVRKSIVGD
                     HRDYLSAAFKEALERVELVIASGGLGPTEDDLTRETVAELLGRKLNLNNEILRYIEGR
                     FRQLGREMPAVNVRQAMVPEGAEVLENPRGSAPGLWIEDAGRCIALLPGPPRELKPLY
                     QEQVLPRLQRRSSNVRMFHRELHVAGMGESSVEQRIKPIYTRHADVNTTILAAPGEIQ
                     IHLRVWTDDAAHAQKTLDEIVQGFEIALADRIFSQDGSSLEEVVAGLLTRNNSTISAA
                     ESCTGGLLAQRLTSIAGSSSYFLGGVVSYSNELKTAWADVPPELIQSKGAVSSEVAIA
                     LAEGIRRQVGSTLGVGITGIAGPGGGSDEKPVGTVHIALSHAGGVKERGVVFPGDREA
                     IRWHASQLALDLVRIHFLYNGGEPPARSAKRS"
     gene            1391..1954
                     /gene="thpR"
                     /locus_tag="VGR03_13050"
     CDS             1391..1954
                     /gene="thpR"
                     /locus_tag="VGR03_13050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEV1995250.1"
                     /translation="MRLFVALEIPSAVRENLAALLKSLRAVSPQTRWVRPENLHVTVK
                     FIGEVPETKLADLRGALAGARCHQPVTLDFRGLGFFPNDEHPRVLWAGIAASPNLNTL
                     AADIEKATEKLGIPREQRPFSPHLTLTRFEPPVLPEKLRAAIQENAARDFGSLRTVQF
                     HLIESKLKPSGAEYTTLESYPFAAAEA"
     gene            1961..2599
                     /gene="plsY"
                     /locus_tag="VGR03_13055"
     CDS             1961..2599
                     /gene="plsY"
                     /locus_tag="VGR03_13055"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV1995251.1"
                     /translation="MLEISIPVILIPCAAYLLGSIPFGLLLAKLFGGKDVRKAGSGNI
                     GATNVARVAGPLAGVFTLVFDTAKGAAAVWLAGRVSDESATWMTIAAFVVLLGHCFPV
                     WLNFKGGKGVATALGVFLALCPLAAVSALLLFVLCVAYWRYVSLGSVAAAAAMPLIIY
                     FLWAPHHAPPIIINVGTLAIALLVIYKHDGNLQRLVEGTEPRFSFGKKKDAE"
     gene            2596..3600
                     /locus_tag="VGR03_13060"
     CDS             2596..3600
                     /locus_tag="VGR03_13060"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1995252.1"
                     /translation="MTKIAILGAGGWGTALAIVLSRSRKPHEISLWVHNAERAESIRS
                     ERENKMYLPGAKLPERVGVHRELEATLSGAQVIVGAVPSAHARAVYAAALPFATAGAS
                     FVSASKGLEPSTHLRMSEVIAQVVTPKFTPRIAVISGPSFALEAARGEPTAVVLASRE
                     AALAAELQEEFAGPNFRLYTNDDVLGVELAGAMKNVMAIAAGACQGLGLGSNALAALI
                     TRGLAEMARLAVALGARPETLSGLAGLGDLVLTCTGSLSRNRFVGIELGKGYPLREIM
                     ANMRMVAEGVHTAAPLLALAREHQIEMPITEQVDAILHAGKFPKDAIRDIMERPLKRE
                     "
     gene            3722..5503
                     /locus_tag="VGR03_13065"
     CDS             3722..5503
                     /locus_tag="VGR03_13065"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEV1995253.1"
                     /translation="MPLDAKSRWERVRAGRVRILWLVLGALLLVSALPIGLYHRQVLQ
                     LSQDKLVDTERVQQTELTRSLAQEIQLFESNLTQQLLSERQILALTGLIDNVEDPAAE
                     PKVTRLLEDFVESNRNTFIYLTAVGKSGKGTSASQGNFRAEQDPFVARALQRAFVTCM
                     QVAKFRSDPIALAPENRPAFVVAVPLKDVNENFTGMLAAVVSLDPIVHRLQDASVRGR
                     TVFLVDHNGRLVAHWDTKNFVPGADVSANSLVAQVKALPQELRSTETVRFSEKGKKHP
                     VEMIGTYSTFPEVNWAVIAQRSLDEAQTDTGVTELNRQALAFVTVVILAAILVGYFFA
                     VGISGPIRGLAASTRAISRGEFHQRSPVRGASEISELAENFNKMASDIEEYIERLKEA
                     AEENRELFIGSIRMLAAAIDEKDPYTRGHSGRVAKYSTLIGQELGLSTEEMDKLRIAA
                     LLHDVGKIGVEDRVLKKPGSLTPEEFGLMKQHTVKGANIMRPVSQLKEMLPGIELHHE
                     HMDGRGYPYGLQGQQIPLMARIIGVADTLDAMTTNRPYQTAMELDYAMNRIRALTGSK
                     FDAVVVTALESAVNAGKLRLSAVEVHV"
     gene            5651..6622
                     /gene="ftsY"
                     /locus_tag="VGR03_13070"
     CDS             5651..6622
                     /gene="ftsY"
                     /locus_tag="VGR03_13070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV1995254.1"
                     /translation="MISLFGKNEPEPTLLERLKKSVSKTKAALSETVDTIFLGERKID
                     PSILNDLETALLSADLGVKPTREILDAVREKMDRNALADAAQLKREIKSHIIRILNAP
                     VRPDVAAGSGISTSQNGSGTRVIFIVGVNGAGKTTSIGKLAHRLRQEGRSVVLCAADT
                     FRAAAVEQLEIWAQRNGIEVIKQKSGADPAAVVYDAVAAAKARSSDVVIVDTAGRLHT
                     KSNLMAELEKMKRTAAKVIPGAPHDVLLVLDATTGQNGLAQAREFTSTVGVTGIILTK
                     LDGTAKGGIVVAISRELGLPIRFVGTGEQINDMVPFDAETYANSLFD"
     gene            6626..7762
                     /gene="ribD"
                     /locus_tag="VGR03_13075"
     CDS             6626..7762
                     /gene="ribD"
                     /locus_tag="VGR03_13075"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV1995255.1"
                     /translation="MDAHSIRFMQHALALARKGAGLASPNPMVGCVIVREGQIVGEGF
                     HQYEWRDHAEIVALKSAGEKTRGATLYVTLEPCNHTGRTGPCTEAIIAAGVQRVVAAM
                     DDPNPVNSGCGLERLRAAGIEVFTGVCEEEARRLNEAFACWIRTKKPFVTLKSAMTLD
                     GQLAMPGERAKFHHKSRVKNPANWISSEESRAEVHRMRHASDALLTGIGTIQVDDPLL
                     TDRSGLPRRRRLLRVILDSGLSISRHSRIVKTADEDLLIFTNQSEGSLRARAIRKEGV
                     EVVFVRSVRGGLHLEGVLADLGRREILSVLLEAGPTLNGAALSAGVVHKLFLFYAPKI
                     AGDTRVPFAHAPKLILPVLQYQNMKQFGPDLAVEGYLLDVYRNH"
     gene            7743..8399
                     /locus_tag="VGR03_13080"
     CDS             7743..8399
                     /locus_tag="VGR03_13080"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV1995256.1"
                     /translation="MFTGIIEHTGTIDALDLKADGGRITIHAPTVAPSLAVSNSIAVN
                     GCCLTVVDLHDYRLSADLSGETIRKTSFGAKAGELKKGTRVNLEQPLTAGKEFGGHFV
                     LGHVDTIGRVTHLTPEGENWWFGVQVPAEFARYIVPKGSITIDGISLTVARWQNSVAE
                     FAVIPYTYEHTNIRDRKPGDAVNLEGDVLGKYVERYLEAREDSTSSKLTIEQLVSQGF
                     "
     gene            complement(8400..9836)
                     /locus_tag="VGR03_13085"
     CDS             complement(8400..9836)
                     /locus_tag="VGR03_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV1995257.1"
                     /translation="MSATANGSCDPIKQLRKEMGFWDVLLFNIATVLGPRWIAAAAHN
                     GTSSISLWVLAAVFFFVPTALVINELSSRFPEEGGLYVWSKEAFGDFHGFVAGWTYWI
                     YTVFYFPGLLLASTAMSGYLAGAGGASLSQNRSFLLIGSFVLLVVAVVLNIIGLNVGK
                     WLQNAGGVSTYLPLLMLIGVAVMLWFHHGSVTHFTWANMMPHWSWDTVNFWSQIAFAF
                     TGLELVSAMSAEIRNPRRTLPRAVLSSGALIAVIYILGTFAVLSLVPASDVDPKSGVF
                     QAITQGSTALRIGFVGVLAALLVSVGNAGGVGSTVAGISRVPFVVGIDRYLPAAFGKI
                     HAKWKTPYVSIIVQAVASGIVLMLSQINETTRGAYQSLVDITVILYFVPFLYMYAAVI
                     KLAGRKDRLENEHAVLIPGGKIGVWLTSGLAFVVTVASIIFSIIPPGEVTNRVFFEVK
                     VVGASIAAIALGLGLYFRGARAKARESV"
     gene            complement(9833..10609)
                     /locus_tag="VGR03_13090"
     CDS             complement(9833..10609)
                     /locus_tag="VGR03_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1995258.1"
                     /translation="MSEEKSINYFEFREVCKSFDERLVLDHVSFTVKRGETCVIMGRS
                     GVGKSVSLKHIMGFLKADSGQIIIDGEDVTDFKEPDFEEVRRKVTMVFQSGALFDSLT
                     VGENMAFPLEGQPGLTAEDVDAYVMKIARMLELESVFDKIPSELSTGMKRAVAMGRAL
                     AQNPEAILFDEPTTMVDPIMAGHMGDLILRLKKAFQKTAIVVTHDTHLAKKLADVVVF
                     LQEGRVTFFGPWADFENSTDPFLKNFRMQDELIPALDVTL"
     gene            complement(10606..11241)
                     /locus_tag="VGR03_13095"
     CDS             complement(10606..11241)
                     /locus_tag="VGR03_13095"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEV1995259.1"
                     /translation="MAAGKSEVGVAGYVLAGGGSTRFGRDKALADVGGTPMLARMIEL
                     LQRVTKQVKIIAVPSKYAAFGAKIVEDRWPGEGPLGGIITALKDAAESPAPREWNLIV
                     SCDMPFLTAEWLTFLGERAAKSKAQVLLPRSAGGPEPLCACYRTDAAEPLLQAFGSGI
                     RKITDALKHLTTEVLDEADWKRFDSAGRLFWNMNTAADYEEARRILEAERK"
     gene            complement(11310..12710)
                     /locus_tag="VGR03_13100"
     CDS             complement(11310..12710)
                     /locus_tag="VGR03_13100"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV1995260.1"
                     /translation="MKTILIVDDEPAARYGLRRALEAKYRIAEADSAEAAREALPREQ
                     PDLVLLDVVLPGQDGLSFLKWMREQGSELPVLMVSALDTAKTAVKALQLGAADYLVKG
                     FELEELRQRVANLLKLVTLEKENDALRRRLTTEGQFGQMIGRSEEMRRAFEMADRVAA
                     TDSTVLILGESGTGKDLLAQEIHARSPRNGKAYVAVNCAALPETLIESELFGFERGAF
                     TGAAQQKKGKFELASGGTIFLDEIGDMNPVTQAKVLRVLENRTIERLGGTQSIPVDVR
                     VISATHRNLQAEIRAGKFREDLFYRLRVVTIDLPPLRAHKEDIALLAETFLQMHGARL
                     GRTARLKREAMAAIEKYDWPGNVRELKNALERSVVLSCGDEIGVEDLPGEVVHGEAIS
                     IRHRGDEHDNGIGERDFREAKRKFEVAYLTRQLAEHRWNVSRTAATIGLHRQSLQEKL
                     RELGIRRPGREPLEEE"
     gene            complement(12707..14689)
                     /locus_tag="VGR03_13105"
     CDS             complement(12707..14689)
                     /locus_tag="VGR03_13105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1995261.1"
                     /translation="MGSLLYIRLIGYTAGTLLQLFWMVVILGYRRQRNFERVFFFLCL
                     ALFFFYGGSLLALNSQIYYPLPPAALNEFAIAIITVGLCLLPALLLHLHMEYAETRGL
                     LKSKRWKAAVFLIFYAACLHLAYHRIPPLLQDLHFNFLEPGSSLGHGFAIVLAVALAW
                     CGGWERRFATAAPDKPQRYFHWTLLVFFAVSVLAVAGLHLAPLHLEARTAGELATAFS
                     LLPILPFSVLIFLVYRHNFLQIGRQKNLLYAISATFLALLYLSLVRRLSLRLEPVLPP
                     EATASILLFVLVIFIEPLQRALGRRLQETAQKEMDLAHRLASEIQQEARRGDVPQLAD
                     FIESRLKEQFEFAEVGLMLWHPGSGEEGRLPARKEFREGFSILHGNQTIGSLLIEPHG
                     AAVSGETRRALEFLCEQLPGALDLCRAIEEKLRLERELAERERLAVLGQMAASISHNL
                     KNPLGSMKTILQVQLENPEMPESMRAETKMILEEIGRLSAKLNQLLQFSRPAVLGEGR
                     PARCNAFEVAREVVGVLRPEAQRKGVALELHPAATALSVAISTEALNDILTNLVVNAL
                     DAAARGGHVHVGVSRKDGNGFVEVEDDGPGVPSELRGKILQPFFTTKTQGTGLGLAIV
                     ARRASDAGGKLEFESPVKNGRGTRFLVWLPLGEETR"
     gene            14956..15276
                     /locus_tag="VGR03_13110"
     CDS             14956..15276
                     /locus_tag="VGR03_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV1995262.1"
                     /translation="MAVNLTPLHDRVVVRRIEEKESIKGGIIIPDSAKEKQQEGEVIA
                     VGAGKREKGERVPLDVKPGDRVLFGKYSGTEIKIEDEELLILKEDEILAKISGAAKAA
                     GSKK"
     gene            15405..17039
                     /gene="groL"
                     /locus_tag="VGR03_13115"
     CDS             15405..17039
                     /gene="groL"
                     /locus_tag="VGR03_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV1995263.1"
                     /translation="MAKQIVTGEASRQAILRGVNALADAVKITLGPKGRNAVIEKKFG
                     APIITKDGVTVAKEIELRDPLENMGAQMVREVASKTSDVAGDGTTTATVLAQAIFREG
                     VKTVAAGASPTALKRGIEKAVEVAVEEIKRLHKDVKGEMIAQVGTISANNDKQIGGII
                     AEAMKKVGKDGVITVEESKTMETTLEVVEGMQFDRGYLSPYFITDPERMECVLEDVRI
                     LIHEKKISSMKDLLPLLEQTAKMGKPLLIIAEDIEGEALATLVVNKLRGTLQCAAVKA
                     PGFGDRRKAMLEDIATLTGGKAVTEDLGIKLENVKLEDLGRAKKITIDKDNTTIIEGG
                     GKSADIEGRVKQIRTQVENTTSDYDKEKLQERLAKLVGGVAVIKVGAATETELKEKKA
                     RVEDAMHATRAAVEEGIVPGGGSALLRAITAVEKLKLHDDEAVGAGIVKRALEEPSRQ
                     IALNAGHEGAVVIGKIRDSKDDNFGFNAETGEYGDMIKMGIIDPAKVTRLALQNAASI
                     AGLMLTTEALIADIKEDSKAAAAGGAPGAGGMGGMY"
     gene            17216..17842
                     /locus_tag="VGR03_13120"
     CDS             17216..17842
                     /locus_tag="VGR03_13120"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HEV1995264.1"
                     /translation="MPGLLQRFLPREEGFFDLFAKQAANIHVGANALHKMLSHYTGVP
                     EQVQIVKAIEHEGDEITHALFTKLNQTFITPFDREDIYELCSRLDDVIDLIDAAASRF
                     VLYRVDSVRNGTIDLVKVLVSATAEVEVAVRALETPQKALKHCIEINRYENDSDRLCR
                     TLIAQLFDEEKDPVQIIKWKEIFEVIETAVDKCEDVANVIEGVILKSA"
     gene            17965..18960
                     /locus_tag="VGR03_13125"
     CDS             17965..18960
                     /locus_tag="VGR03_13125"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEV1995265.1"
                     /translation="MSSFSLLFVVIFLTLAFEFSNGWHDAANSIATVVSTRVLTPFRA
                     VVWAAFWNFIAAFVFGTAVAKTIGKGMVHIEMVDEKVLLAGLLGAITWNTITLILGLP
                     TSSSHALMGGYGGAAVAHAGLKALVLGGWTKPVLFIFLSPIIGFFVAVGVTILTSWIV
                     RRQRPLKVDRWFRRLQLLSAAGYSLGHGTNDAQKGMGIITTALVAGGLMKTYDVPYWV
                     IICCHLAMAGGTMAGGWRIIKTMGQRITKLTPFGGFAAETAGAVTLVATAHFGIPVST
                     THTITGAIVGVGATRRLSAVRWGVTRRIVFAWVLTIPGAALFGAALFQLVHHWLR"
     gene            complement(19160..19585)
                     /locus_tag="VGR03_13130"
     CDS             complement(19160..19585)
                     /locus_tag="VGR03_13130"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEV1995266.1"
                     /translation="MSAKTFVDTNILIYAHDADAKAKHEVAKSVLRELWSEHTGILSM
                     QVLQEFYVNVTRKIATPLPKDLARAVVSSYTAWCMETTPAEMAAAFRIEDDSRVGFWD
                     ALIVSSAVKSGAGRILSEDLNAGQRIAGILIENPFAGVR"
     gene            complement(19575..19808)
                     /locus_tag="VGR03_13135"
     CDS             complement(19575..19808)
                     /locus_tag="VGR03_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HEV1995267.1"
                     /translation="MKQNVTISLDRQTIRKAKIMAARRDTSISGLLAQQIEMLVGEEE
                     AYERAERQALALLETGFRLGGVIRAKRDEWHER"
     gene            complement(20142..20630)
                     /locus_tag="VGR03_13140"
     CDS             complement(20142..20630)
                     /locus_tag="VGR03_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995268.1"
                     /translation="MRRLFVMCGIVCFAAATASAQTKVTGTAQFGKAEGQQMLPAGDR
                     ADHSLGIGQRKCTWTKPQEIGGDKTKEGVSTESMDISGNNIRSRGMHVTTMESGDKYF
                     VSYQGTGTLKDGALQSGKGTWMYTGGSGKLKGIKGKGTYTCAASGDSVSCDIEGEYQL
                     AK"
     gene            complement(20900..>21310)
                     /locus_tag="VGR03_13145"
     CDS             complement(20900..>21310)
                     /locus_tag="VGR03_13145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1995269.1"
                     /translation="QNQQAGAAQTYVGVAGEAIIGYHTLAVGQVTLEDSPERLKKGLA
                     KHPVPIMLLARLAIDHRWQGQGVGKALLRDAMQRTLQAADIAGIRAFAVHAKDEEARR
                     FYEHFDFIPSPTDPMHLFVLLKDVRRILLGSRGG"
BASE COUNT         4401 a         6797 c         5869 g         4243 t
ORIGIN      
        1 tgggcatggc gctgcagcct tccggccgcg cgattcttgc caccaccgac gccctcttca
       61 cgctcgattg ggacgtccgc ggcttgccat tgatcggata attgaagaga tgaaagcgga
      121 aatcatcgcg gttggttcgg aactgttgac tccggaccgc atggacacga actcgctctt
      181 cctgaccgaa gagctgaaca agctgggcat cgaggtggtg cgcaaatcga tcgtggggga
      241 ccatcgcgat tatttgagcg ccgcattcaa ggaagcgctg gagcgcgtcg agctggtcat
      301 cgcatcgggt gggcttggtc ctacggaaga cgacttgacg cgcgaaaccg tcgcggaact
      361 tctgggacgg aagttgaacc tcaacaacga aatcctgcgc tacatcgaag gtcgcttccg
      421 gcagctcggc cgggagatgc ctgcggtgaa tgtgcggcag gcgatggtac cggaaggcgc
      481 ggaggtgctg gagaatccgc gtggctcggc gccgggactg tggatcgaag acgcggggcg
      541 ctgcatcgcg ctgctgcccg gcccaccgcg cgaattgaag cccctgtatc aggagcaggt
      601 tttgccgcgg ctgcaacggc gcagttcaaa cgttcgaatg tttcatcgag aattgcacgt
      661 cgcgggcatg ggtgaatcaa gcgtcgagca gcgcatcaag cccatttaca cgcgtcatgc
      721 cgacgtgaac accacgattc tcgccgcgcc gggcgagatt cagatccatt tgcgggtgtg
      781 gaccgacgac gcggcgcacg cgcagaaaac gctggacgaa atcgtgcagg gattcgaaat
      841 cgcgctggcc gaccgtattt tttcgcaaga cggttcctcg ctggaggaag tggttgcggg
      901 cctgctgacg cgaaacaact caaccatttc cgccgcggaa agctgcaccg gcggcttgct
      961 ggcgcagcgg ctgacgagca tcgcgggcag ctcctcgtat ttcttgggtg gcgtcgtgag
     1021 ctacagcaac gagctgaaga cggcctgggc ggatgttccg ccggaactca tccagtccaa
     1081 aggcgccgtc agcagtgaag tggcgatcgc gctggccgaa ggcattcgcc gccaggtcgg
     1141 cagcacgctg ggcgtgggca tcaccggcat tgccggaccg ggcggcggca gcgatgaaaa
     1201 gcccgtgggc accgtgcaca tcgcgctctc gcatgccggt ggcgtcaaag agcggggcgt
     1261 ggtcttcccg ggcgaccgcg aagccatacg ctggcatgcc tcgcaactgg cgctcgattt
     1321 ggtgcgcatt cattttcttt acaacggcgg ggagccgccg gcgcgcagtg caaaaaggtc
     1381 ctgacctccc atgcgtctgt tcgtcgcgct ggagattcca tccgcggtgc gagagaatct
     1441 cgcggccttg ctcaaatctc ttcgcgcggt ctcgccgcaa acgcgctggg tgcgtccgga
     1501 gaatcttcac gtgacggtca aattcattgg tgaagtgccg gaaacaaaac tcgcagacct
     1561 tcgcggcgct ctcgcgggcg cgcgctgcca tcaacccgtc acgctcgatt ttcgcggact
     1621 cggctttttc cccaacgatg aacatcctcg cgttctctgg gcgggcatcg cggcttcgcc
     1681 gaacctgaat actctggcgg ccgatatcga gaaggccacg gaaaaacttg gcatcccgcg
     1741 ggaacagcgg ccattttcgc cgcatctgac actcacgcgg ttcgagcctc ccgtgctgcc
     1801 ggaaaaactt cgcgccgcga tccaggaaaa cgcggcgcgc gattttggtt cgctgcgcac
     1861 cgttcaattt catctgatcg aaagcaagct aaaaccctcc ggcgcggagt acactacgct
     1921 cgaaagttat ccgttcgcgg cggcggaggc ctgacggagc atgctggaga tttcgatccc
     1981 tgtcattctg atcccatgcg ccgcgtatct tcttggctcg attcccttcg gacttctcct
     2041 cgcaaaatta tttggcggca aggacgtgcg caaagccggc agcggcaaca tcggcgcaac
     2101 caacgtcgcg cgtgtcgcgg gccccttggc gggagttttc acgctggttt tcgacaccgc
     2161 gaaaggcgcg gcagccgtct ggctcgccgg ccgtgtcagc gacgaaagcg ccacctggat
     2221 gacgatcgcc gctttcgttg tactgctcgg acactgcttc ccggtatggc tcaatttcaa
     2281 aggtggcaaa ggcgtagcca ccgctcttgg cgtgtttctg gctctgtgcc cgctggcagc
     2341 ggtcagcgcc cttttgcttt tcgttctttg cgtagcctat tggcgttatg tttcgctcgg
     2401 ctctgtcgcg gcggccgcgg ccatgccgct gatcatctat tttctgtggg cgccacatca
     2461 tgcgccgccc atcatcatca atgtggggac gctggccatc gctctgctcg tcatttacaa
     2521 gcatgacggc aatcttcagc gcctcgtcga aggcaccgaa ccgcgctttt cctttggcaa
     2581 gaagaaggac gcggaatgac gaagatcgcc attctcggcg cgggaggctg gggcacggcg
     2641 ctggcgatcg tgctttcgcg cagccgcaag ccgcacgaaa tctcgctttg ggtgcacaac
     2701 gccgagcgcg cggaatcaat ccgcagcgag cgtgagaata agatgtatct tcccggcgcg
     2761 aaattgccgg agcgtgttgg agttcatcgt gaattggaag ccacgctttc tggagcacag
     2821 gtcatcgtcg gcgccgtgcc ctccgcacat gctcgcgccg tttatgctgc cgcacttccg
     2881 tttgccaccg ccggcgcgag tttcgtcagc gccagcaagg gccttgagcc atcaacgcac
     2941 ctgcggatga gcgaagtgat cgcgcaggtc gtcacgccga aatttacgcc gcgcatcgcc
     3001 gtgatttccg ggccatcgtt cgccctggag gccgctcgcg gcgaacccac ggccgtcgtt
     3061 ctggcctcgc gggaagccgc gctcgccgcc gagctgcagg aagagtttgc gggcccaaac
     3121 ttccgtctct ataccaatga cgacgttctg ggcgtggaac tcgccggcgc gatgaaaaat
     3181 gtgatggcca ttgctgcagg cgcctgccag gggctggggc tcggctcgaa cgctctggcg
     3241 gcgctgatca cgcgcggcct cgcggaaatg gcccgtttgg cggtggcgct aggggcgcgg
     3301 ccggaaacgc tcagcggtct ggcaggattg ggcgatttgg ttctcacctg cacgggctcg
     3361 ctcagccgca atcgatttgt ggggatcgag cttggcaaag gatatcctct ccgcgaaatc
     3421 atggcgaata tgcgcatggt tgccgagggc gtgcataccg ccgcgccgtt gctggcactg
     3481 gcccgcgaac atcagattga aatgccgatc accgagcagg ttgacgccat cctacacgcg
     3541 ggcaagtttc ccaaggacgc gattcgggac atcatggagc ggcccttgaa gcgggagtag
     3601 cgaaagaccg ggattgtcca attttccgcc tctagtacta aagtcccagt tgcacatgcg
     3661 cgacgcggag cgctagctga ccgcctaagt cccccaggtt ctcctagaat gaatagtcga
     3721 gatgccgctg gatgccaaat ctcgctggga acgtgtgcgc gccgggcgcg tgcgcatcct
     3781 ttggctcgtt ctgggagcgc tcttgctggt cagcgcgctt cccattggtc tctaccaccg
     3841 ccaggtattg cagcttagcc aggacaagct ggtggatacc gaacgcgtcc agcaaaccga
     3901 actgacgagg tcgctggcgc aggaaattca gctattcgaa tccaacctga cgcaacagct
     3961 cctgagcgag cgccaaattc tcgcgctcac gggtctcatc gacaacgtcg aagatccggc
     4021 ggcagaaccc aaggtcaccc gcctgctgga agattttgtc gagagcaatc gcaacacgtt
     4081 catttatctg accgccgtgg gcaagtccgg aaaaggcacc agcgcgtcgc agggaaattt
     4141 ccgggccgag caagatcctt tcgttgcaag agcactgcag cgcgcgttcg ttacgtgcat
     4201 gcaggtggcc aagtttcgca gcgatcctat tgcgttggcg ccggaaaacc ggccggcgtt
     4261 cgtcgtcgcc gttcctttga aggatgtgaa cgaaaacttt acggggatgc ttgcggcggt
     4321 ggtttccctg gatcccatcg ttcaccgtct gcaggatgcc agcgtacggg gacgcaccgt
     4381 ttttctagtg gaccacaacg gccgcctcgt ggcgcactgg gacacaaaaa acttcgttcc
     4441 aggcgcggac gtcagcgcca attcgctggt cgcgcaggtg aaggcgctgc cgcaggagct
     4501 gcgcagcaca gaaacggttc gcttttccga aaaaggtaag aaacatccgg tcgagatgat
     4561 cggaacctac agcacttttc ctgaagtgaa ttgggcggtc attgcccaga ggagtctcga
     4621 tgaagcgcag accgacaccg gcgtcaccga gctgaaccgg caggcgctgg cgttcgtcac
     4681 cgtcgtcatc ctggccgcta ttctcgtcgg atatttcttt gcggtgggca tcagcgggcc
     4741 catccgcggt ttggcggcct ccacgcgcgc gattagccgc ggtgaatttc atcaacgctc
     4801 tcccgtccgc ggcgcttcgg agatcagcga gctcgctgaa aacttcaaca agatggccag
     4861 cgacatcgag gaatacatcg agcgcctgaa ggaagccgcc gaggaaaacc gcgagctgtt
     4921 cattggatcg atccgcatgc tcgccgcggc catcgacgaa aaggatccgt acacgcgggg
     4981 tcactccggc cgcgtggcga agtattcgac gctcatcggc caggagctgg gactgtccac
     5041 ggaggagatg gacaaactgc gcattgcggc gttgctgcac gacgtcggaa aaatcggtgt
     5101 cgaagaccgc gtgctaaaaa agcccggctc gctgacgccc gaagaatttg gcttgatgaa
     5161 gcagcacacc gtgaaaggcg cgaacatcat gcgcccggtt tcgcagctca aagagatgct
     5221 ccctggcatc gagctgcacc acgagcacat ggacggccgc ggttatccct acggcttgca
     5281 gggccagcag attccgctga tggcgcgcat tatcggcgtg gctgacactc tcgacgccat
     5341 gaccaccaac cgcccctatc agacggcgat ggaactggac tacgccatga accgcattcg
     5401 cgccctgacc ggctccaaat ttgatgcggt ggttgtgacc gctcttgagt ccgccgtcaa
     5461 cgccggcaag ctccgcctta gcgccgtcga agtgcacgtt tagctgcgat cctgccaact
     5521 cgtctcaccc tctcttccgt cagtgtgcct gatacggtct ggtgcgcctt cacacgtctc
     5581 tggtaacggt ccacgaggcc gcgcatctgc tacaatttta agtcgccgtt actgtcatgc
     5641 gcgcagcctt atgatttcac tttttggcaa gaatgaaccg gagccgacac tccttgagcg
     5701 gctgaagaag tccgtcagca agaccaaggc ggcgctctcc gaaaccgttg atacgatttt
     5761 tctcggcgag cggaagatcg atccttcgat cctgaatgat ctggagacgg cgctcctctc
     5821 tgccgacctg ggcgtgaagc cgacgcgcga gattctggat gcggtgcgcg aaaagatgga
     5881 ccgtaacgcg cttgccgacg ccgcgcagct caagcgtgag atcaagtcgc acatcattcg
     5941 cattctgaac gcgcctgttc ggccggatgt ggcggctgga agcggcatct ccacctcgca
     6001 gaacggcagc ggcacgcgcg tgattttcat cgttggcgtc aatggcgcgg gcaagaccac
     6061 cagcatcggg aaactcgcgc accggcttcg ccaggagggc cgcagcgtgg tactctgcgc
     6121 cgcggacacg tttcgcgcgg ccgcagtcga gcaactggaa atctgggcgc agcgcaacgg
     6181 catcgaagtc atcaaacaga aatcaggcgc cgaccctgcc gcggttgttt acgacgccgt
     6241 cgccgccgca aaagcgcgct cgtccgacgt cgtcatcgtg gataccgcgg gacgcctgca
     6301 caccaagtcg aacctcatgg ccgaactcga aaagatgaag cgcaccgccg cgaaagtgat
     6361 tcccggcgcg ccgcacgatg ttttgctggt tctcgatgcc accaccgggc agaacggtct
     6421 tgctcaggcg cgcgaattca cttccaccgt gggcgtcacc ggaatcatcc ttacgaagct
     6481 cgatggcacc gccaagggcg gcatagttgt cgcgatctcg cgcgagctgg gcctgcccat
     6541 ccgcttcgtg ggcaccggcg agcagatcaa cgacatggtc ccgttcgacg ctgaaacgta
     6601 cgccaactct cttttcgatt aaaccgtgga cgcccactcc attcgcttca tgcaacacgc
     6661 gctggccctg gcccgcaaag gcgccggcct cgccagtccc aaccccatgg tgggttgcgt
     6721 catcgtgcgt gaaggccaga tcgtcggcga aggttttcat cagtacgaat ggcgcgacca
     6781 cgcggaaatc gtcgcgctga aatccgccgg tgaaaaaacg cgcggcgcaa cgctatacgt
     6841 cacgctcgaa ccttgcaatc acaccggccg caccgggccc tgcaccgaag cgattatcgc
     6901 tgcaggcgtt cagcgcgtcg tcgcggccat ggatgatccg aatcccgtta attccggctg
     6961 cggccttgag cgcctccgcg cggcgggcat tgaagttttc accggtgttt gcgaagagga
     7021 agcgcgccgc ctcaacgagg cattcgcatg ctggattcgc acgaagaaac ccttcgtcac
     7081 gctgaagtcg gcaatgacgc tggacggcca gctcgccatg cccggtgaac gcgcgaaatt
     7141 tcaccacaaa tctcgcgtga agaaccccgc aaactggatt tcctccgagg aatcccgcgc
     7201 cgaagttcac cgcatgcgcc acgcctccga tgcgcttctg accggcattg gcacgataca
     7261 agtggatgat ccgcttctta ccgatcgcag cggcctcccg cgccgccgcc gtttgttgcg
     7321 cgtcatcctg gactccgggc tgtcgatttc tcgccactca cgcatcgtga aaacagccga
     7381 tgaagacctg ctgattttca cgaatcaaag cgagggttcg ctaagggcgc gagctatcag
     7441 gaaagaaggc gtcgaggtcg tcttcgtacg atcggtgagg ggcgggctcc atttagaagg
     7501 tgttcttgcc gacctgggcc gccgcgaaat cctcagcgta ctgctggaag ccggcccaac
     7561 gttaaacggt gcggcgcttt ccgcaggagt cgtgcataaa ctttttcttt tctacgcgcc
     7621 aaaaatcgcc ggcgacaccc gcgttccttt tgcgcatgcg ccaaagttga ttcttcccgt
     7681 gcttcaatat cagaatatga agcagttcgg cccagacctc gccgtcgaag gatacctgct
     7741 agatgtttac cggaatcatt gagcacaccg gcacgatcga cgcgctcgac ctgaaagctg
     7801 acggcgggcg catcaccatc cacgcaccga ccgtcgcacc ctcgctcgct gtttcgaaca
     7861 gcatcgctgt gaatggatgc tgcttaaccg tagtcgatct ccacgactac cggctctccg
     7921 ccgatctatc cggtgaaacg atccgcaaga cttcgttcgg cgcaaaggcc ggcgaattga
     7981 aaaaaggcac gcgcgtcaat cttgaacagc cgctcaccgc gggcaaggaa tttggcggac
     8041 attttgtcct cggccacgtg gacaccatcg gccgagttac gcatcttacc cccgaaggtg
     8101 aaaactggtg gtttggagtg caggtccccg cggaattcgc tcgttacatt gtgccgaaag
     8161 gctcgatcac cattgacggc atcagcctca ccgtggcccg ctggcagaac agcgtcgccg
     8221 aattcgccgt gattccctac acctacgaac acaccaacat ccgcgaccgc aaacccggcg
     8281 acgcggtgaa cctcgaaggc gatgtgcttg gaaaatacgt cgaacgatat ctggaagcgc
     8341 gcgaagattc aacgtcgagc aaactgacga tcgagcaact ggtcagtcag ggtttctagc
     8401 taaacggatt cgcgtgcctt ggcgcgcgcc ccgcggaaat ataatccgag cccaagcgca
     8461 atcgccgcaa tcgatgcgcc gacaaccttc acctcaaaga acactctatt ggtcacctcg
     8521 cccggcggga tgatggagaa aataatactg gcgaccgtga cgacaaaagc cagcccgctg
     8581 gtgagccata cgccaatctt gccgcccgga atcagaaccg cgtgctcgtt ttcgaggcga
     8641 tccttgcggc ccgcgagctt gatcaccgcg gcatacatgt agagaaacgg aacgaagtac
     8701 aagatcaccg tgatgtccac gaggctttgg taggccccgc gggtcgtttc gttgatttga
     8761 ctgagcatga gaacgatccc ggaggccacg gcttgcacga tgatcgaaac gtacggtgtt
     8821 ttccacttgg catgaatctt cccaaacgcc gccggtagat accggtcaat gccgacaaca
     8881 aaaggcacgc gcgatattcc cgctacggtg cttcccacgc cgcctgcatt gcccacggaa
     8941 accaggagcg ccgcgagcac gccaacaaat ccgatccgta gcgccgtgga gccctgcgtg
     9001 atcgcctgaa acaccccgct cttcggatcc acatccgacg cgggaaccag cgagagcacc
     9061 gcgaaggtgc cgaggatata gatcaccgcg attaacgctc ctgagcttaa aaccgcccgc
     9121 ggcagtgtcc gccggggatt tctgatctcc gcgctcatgg ccgacacaag ttccagaccc
     9181 gtgaacgcaa aagcgatctg cgaccagaaa ttcacggtgt cccagctcca gtgcggcatc
     9241 atgtttgccc acgtgaagtg cgtcacggat ccgtgatgaa accagagcat caccgctacg
     9301 cctatgagca tcagcaaagg aagataggtg gacacgccgc cggcgttttg cagccacttc
     9361 ccgacattca gcccgatgat gttgaggacg accgccacga cgagcaggac aaaagatcca
     9421 ataagcagaa atgatctgtt ttgcgagagc gatgcgcctc ccgcgccggc gagatagccg
     9481 ctcatggccg tgctcgccag caggaggccg ggaaaataaa aaacggtata gatccaataa
     9541 gtccaaccgg cgacgaagcc gtggaaatca ccaaaggctt ccttggacca gacgtagagg
     9601 ccgccctcct cgggaaaacg cgacgaaagt tcgttgatga ccagcgccgt cggaacgaag
     9661 aaaaataccg ctgccagcac ccagaggctg atcgaagaag tgccgttgtg tgccgcggcg
     9721 gcaatccagc gcggacccag caccgtcgca atgttgaaga gcaggacatc ccagaagccc
     9781 atctccttgc gcaactgctt aatcggatcg caggagccgt ttgctgtcgc actcacaaag
     9841 tcacgtccag cgcgggaatc aattcatcct gcatgcggaa gttcttgagg aaaggatcgg
     9901 ttgagttttc aaaatcggcc cagggtccga agaaagttac tcggccttcc tgaagaaaga
     9961 cgaccacgtc ggcaagcttc ttcgccagat gcgtgtcgtg cgtcacgacg atggctgtct
    10021 tttgaaatgc tttcttaagc cgcaggatca aatcgcccat gtgccccgcc atgatcggat
    10081 ccaccatcgt ggtcggctca tcgaacagga tcgcttcggg attctgcgcg agcgcgcgcc
    10141 ccatggccac cgcacgcttc atcccggtgg aaagttcgct gggtattttg tcaaagacgc
    10201 tttctagttc cagcatgcgc gcgattttca ttacgtacgc gtcaacgtcc tccgcggtca
    10261 ggcccggctg cccttccagc gggaacgcca tgttctcgcc gaccgtgagc gagtcgaaca
    10321 gcgcgccaga ctgaaagacc atggtgacct tgcgccgcac ttcttcgaag tccggctcct
    10381 taaaatccgt gacgtcctct ccatcgataa taatctggcc cgaatccgcc ttcaggaaac
    10441 ccatgatgtg tttcaacgaa accgacttgc ccacgccgct gcgccccatg atcacgcagg
    10501 tctctccgcg cttcaccgtg aagctgacgt gatcgagaac caggcgctcg tcgaagctct
    10561 tgcacacctc gcgaaactcg aagtagttaa tcgacttttc ttcgctcact tgcgctccgc
    10621 ttccagaatc cgccgcgctt cctcgtaatc cgccgccgta ttcatattcc aaaacagccg
    10681 ccctgcgcta tcaaatcgtt tccagtccgc ctcgtcaagc acttccgtgg tcaagtgttt
    10741 cagtgcgtcc gttattttgc ggattccgct gccgaaggct tgtagcagcg gttccgcggc
    10801 atcggtccgg taacaagcgc acaatggctc cggtccgccc gccgagcgtg gcagcaggac
    10861 ctgtgccttg ctcttcgccg ctcgttcacc caaaaacgtc agccattccg ctgtcaagaa
    10921 cggcatgtcg cagcttacga tcagattcca ttcgcggggc gcaggactct ctgctgcgtc
    10981 tttcagcgcc gtaataatgc caccgagcgg cccttctccc ggccatcggt cttcgacaat
    11041 tttcgctccg aatgcggcgt atttgcttgg caccgcaatt attttcactt gtttcgtgac
    11101 gcgctgcagc agctcgatca tgcgcgccag catcggcgtc ccgcccacat ccgcaagcgc
    11161 cttgtcccgc ccaaatcgcg tgctcccgcc tcccgccaaa acgtatccag cgactcccac
    11221 ctcacttttg ccggcggcca ttctctaact ctccgcttcc cttctcttct ctgtgccctc
    11281 tgtgttctcc gtggtctctg tgtaaattct tattcttctt ccagcggctc gcgtcccggc
    11341 cgccggatgc ccagttcgcg caatttctcc tgcaaactct gccggtgcaa tccgatcgtc
    11401 gccgcggttc gcgagacgtt ccaacgatgc tccgccaatt gcctcgtcag ataggccacc
    11461 tcgaattttc gcttggcctc acgaaaatcc cgttcgccaa ttccgttgtc atgctcgtcg
    11521 ccgcgatgcc tgatcgagat cgcctcgccg tgcaccacct cgccaggcaa atcttcgacg
    11581 ccgatttcgt cgccgcaaga cagcaccacg ctccgctcca gcgcgttctt caactcccgc
    11641 acattgcccg gccaatcgta tttttcgatc gccgccatcg cctcgcgctt cagccgcgcc
    11701 gtccgcccca gccgcgcccc gtgcatttgc agaaaagttt ccgccagcag cgcgatatct
    11761 tctttgtgag cccgcagcgg cggcaggtca atggttacga cgcggagacg gtagaacaaa
    11821 tcctcgcgaa atttccccgc gcgaatctcc gcctgcagat tgcggtgcgt cgcgctgatt
    11881 acccggacat ccaccggaat cgattgcgtc ccgccgagcc gttcgatggt gcgattctcc
    11941 agcacgcgca acaccttggc ctgcgtcacc ggattcatgt cgccaatttc atccaggaaa
    12001 atcgttccgc cggaggccag ctcgaacttc ccttttttct gctgcgccgc gccggtaaac
    12061 gcgccgcgct cgaaaccaaa taattccgac tcgatcagcg tttccggcag cgccgcgcaa
    12121 ttcaccgcga cataggcttt gccgtttcgt ggcgagcgcg cgtgaatctc ctgcgccagc
    12181 aaatcctttc ccgtgccgct ctctcccaaa atcagcaccg tcgaatccgt cgccgccacg
    12241 cggtccgcca tttcgaacgc gcggcgcatc tcctccgagc gcccgatcat ctggccgaat
    12301 tgcccttcag ttgtcaaacg ccgccgcagc gcgtcattct ctttttcgag tgtcacgagc
    12361 ttcagcagat tggccacgcg ctgccgcaac tcttccagtt cgaatccttt caccagatag
    12421 tcagccgcgc caagctgcag cgctttaacc gccgtcttcg ccgtgtccag cgccgaaacc
    12481 atcagcaccg gcaattcgct cccctgctcg cgcatccact tcaagaatga caaaccgtcc
    12541 tggcccggca acacgacgtc tagcagcacc aggtccggtt gctcgcgagg aagcgcctct
    12601 cgcgccgctt ccgccgaatc cgcctccgcg atccgatact tcgcttcaag cgcacgccgc
    12661 agcccgtatc tcgccgcggg ttcgtcgtcc acaatcaaaa tagtcttcat cgcgtttctt
    12721 ctccaagagg aagccacacc aggaaccgcg tgccccgccc atttttcacc gggctctcga
    12781 attcgagctt gccgcccgca tcggacgcgc ggcgggccac gatcgccaat cccagacctg
    12841 ttccctgcgt ttttgtcgtg aaaaatggct gaagaatttt tcctcgcagc tccgaaggaa
    12901 ctccgggccc atcatcttcc acctcgacga agccgttgcc gtctttccgc gaaactccca
    12961 catgcacatg cccgcctcgc gctgccgcgt ccaacgcgtt caccaccagg ttcgtcagaa
    13021 tgtcgtttag cgcctcggtg ctgatggcca cactcaacgc tgtggccgcc ggatggagtt
    13081 ccagcgcaac gcctttgcgt tgcgcctcgg ggcgtaagac accgacgacc tcccgcgcca
    13141 cctcgaacgc gttgcatcta gcgggccttc cctctccgag cacggccggc cggctaaatt
    13201 gcagcaactg attcaacttc gcggaaagac gcccgatctc ctcaagaatc attttcgttt
    13261 cggcgcgcat ggactccggc atttccggat tttccagttg cacttgcaga attgtcttca
    13321 tcgatcccag cggattcttc aagttgtgag aaatgctcgc ggccatctgc ccgagcaccg
    13381 ccaaccgttc acgctccgcc aattccctct ccaatcgtaa cttctcctcg atcgcccggc
    13441 acaaatccag cgcccccggc aactgctcgc acaagaattc cagcgctctc cgagtctctc
    13501 cggataccgc cgctccgtgc ggttcgatga gaagcgagcc gatcgtctga tttccatgga
    13561 ggatcgagaa gccttctcga aattccttgc gggcgggaag ccgcccctcc tctccgctgc
    13621 ccggatgcca gagcatcaat cccacttcgg caaactcaaa ctgttctttc aggcggctct
    13681 cgatgaaatc cgccagctgg ggcacatcgc cccgccgcgc ctcctgctgg atttctgacg
    13741 cgaggcgatg cgccagatcc atctccttct gcgcggtctc ctgcagccgc cggcccagag
    13801 ctctctgcaa cggctcgata aaaatcacca gcacgaacag caggatggac gccgtcgctt
    13861 cgggaggtaa taccggctcc agccgaaggc tgaggcggcg gacgagactg aggtagagca
    13921 aggccaggaa agtggccgaa atcgcgtaca aaaggttctt ctgccggccg atctgcaaaa
    13981 agttatgccg atagacaaga aaaataagca cgctgaatgg caagatcggc agcagcgaga
    14041 aagccgtggc aagttccccc gccgtccgcg cttccagatg caacggagcc aaatgcaagc
    14101 cagcaaccgc cagcacggaa acggcgaaaa atactagcag cgtccagtga aaatagcgct
    14161 gtggcttgtc cggggctgct gttgcgaagc gtctttccca tcctccgcac cacgccaacg
    14221 ccactgcgag cacgatggcg aagccgtgcc cgagcgagct gccgggctca agaaaattga
    14281 aatgcaggtc ctgtagaaga ggcggaatcc ggtgatacgc aagatgcaga caagccgcat
    14341 aaaaaatcaa gaacactgcc gctttccatc gcttgctctt gagcaggccg cgcgtttccg
    14401 catattccat gtgcagatgc agcaggagcg cgggcaacag gcaaagtccc accgtgatga
    14461 tggcgatggc gaattcattg agcgccgccg gtggcagggg gtagtagatc tgtgagttca
    14521 gcgcgagaag tgacccgcca taaaagaaga acaatgccag acacagaaaa aagaagacgc
    14581 gctcaaagtt gcgctggcgg cggtagccca gaatgaccac catccagaag agctgtagca
    14641 gcgtccccgc cgtgtagccg atgagccgaa tatagagcag tgaacccatg ggactccaac
    14701 gtgagggatt ctacgccgag gctatctgca gtacaatctc caagtgtcat atttttcttg
    14761 caccacgtaa actattctag gtaaacagct taaatcgaat caatatccac aggagggcca
    14821 ccgagctgtc cgggaaacgc ttgacatgac aggagtttgt gctatattag cagtcggctg
    14881 tgaggagtgc taattcccct ttcttgctcc cagccgcagt tcaaaccgca agttcaccta
    14941 tggaggtttt tcaagatggc agtgaatcta accccgctcc acgaccgcgt agtcgtgagg
    15001 cgcattgaag agaaagaaag catcaaaggc ggcatcatta ttccggactc cgccaaggag
    15061 aagcagcaag aaggcgaagt catcgccgtc ggcgctggca agcgcgagaa gggcgagcgc
    15121 gtgcctctag atgtcaaacc gggcgaccgc gtccttttcg gcaagtacag cggtacggaa
    15181 atcaaaatcg aagacgaaga gctgttgatc ctgaaagagg acgagatcct ggcaaaaatc
    15241 agcggcgctg ccaaagcggc agggtcgaaa aagtaaggtt tcgtcgggcc cgtggtttgg
    15301 cagtcttgaa aaacaacctc aggcctaaag gattgagcaa cagatggcag gtgaaaacct
    15361 gccactcgca aacgaattga aacatttcag gaggaatgaa aagtatggcg aagcagattg
    15421 taacaggtga agcgtcccgc caggcgattt tgcgcggcgt aaacgctctt gccgatgcag
    15481 tgaaaatcac cctcggcccc aagggccgca atgcggtgat tgagaaaaag ttcggcgcgc
    15541 cgatcatcac caaggacggc gtgaccgttg ccaaggaaat cgagctccgc gatcccctcg
    15601 agaacatggg cgcgcagatg gttcgcgaag tcgcttccaa gacttccgat gttgccggtg
    15661 acggcaccac caccgcgacc gtgctcgcac aagccatctt ccgtgaaggc gtgaagactg
    15721 ttgccgcagg cgccagtccc accgctctca agcgcggcat cgagaaggcc gtcgaagtgg
    15781 ccgtcgaaga gatcaagcgg ctccacaaag acgtcaaggg cgaaatgatc gcccaggttg
    15841 gcaccatttc cgccaacaat gacaagcaga tcggcggcat catcgccgaa gccatgaaga
    15901 aggtcggcaa ggatggcgtc atcaccgtcg aggaatccaa gacgatggag accaccctcg
    15961 aagtagtcga aggcatgcag tttgaccgcg gctacctctc cccctacttc atcaccgacc
    16021 ccgagcgcat ggaatgcgtt ctcgaagacg tgcgcatcct catccacgaa aagaaaatca
    16081 gctcgatgaa ggacttgctg cccctgctcg agcagaccgc caagatgggc aagcctctcc
    16141 tcatcatcgc ggaagacatc gaaggcgaag ccctcgccac gctggttgtg aacaagctcc
    16201 gcggcaccct gcagtgcgcc gccgtcaagg cccctggctt tggcgaccgc cgcaaggcca
    16261 tgctcgaaga catcgcgacc ctgaccggcg gcaaggccgt aaccgaagac ctcggcatca
    16321 agctcgagaa cgtcaagctt gaggacctcg gccgcgccaa gaagatcacc atcgacaagg
    16381 acaacaccac gatcatcgag ggcggcggca agtccgcgga catcgaaggc cgtgtgaagc
    16441 agatccgcac gcaggtcgag aacaccacca gcgactacga caaggaaaag ctccaggagc
    16501 gccttgccaa gctcgtcggc ggcgtcgccg tcatcaaagt cggcgcagcc accgaaaccg
    16561 agctgaagga aaagaaggcc cgtgtggaag acgcgatgca tgccacccgt gcggctgtcg
    16621 aggaaggcat tgtccccggc ggcggctcgg cgctgcttcg cgccatcacc gcagtcgaga
    16681 agctcaagct ccacgatgac gaagccgttg gcgcaggcat cgtgaagcgt gccctcgaag
    16741 agccttcccg ccagatcgct ctcaacgccg gccatgaagg cgccgttgtg atcggcaaaa
    16801 tccgcgattc caaggacgat aacttcggct tcaacgccga gaccggcgag tacggcgaca
    16861 tgatcaagat gggtatcatc gacccggcca aggtcacccg cctcgcgctg cagaacgcag
    16921 cgtccatcgc gggcctcatg ctcaccaccg aagccctcat cgccgacatc aaggaagaca
    16981 gcaaggcggc ggcagccggc ggagcccccg gtgcaggcgg catgggcggc atgtactaag
    17041 ttcaccagct cgcccgtaac ctcaatcccg agagggcgtt ccggaccacc ggaacgccct
    17101 cttgcatttg gcaatcccga atccctaacc taagaacgcc tgcttgccta cccttgcccg
    17161 cctctgttat cttccccttg cctcactcag actcttcagg ggagagtcag acatcatgcc
    17221 aggacttttg cagcggttct tgccgcgtga agagggtttc tttgacctct ttgccaaaca
    17281 agcggcgaat atccatgtag gcgccaatgc cctgcataaa atgctctcgc actacaccgg
    17341 ggtgccggaa caagtgcaga tcgtaaaagc catcgagcac gaaggcgacg agattaccca
    17401 cgccctcttc accaaactga atcaaacctt tatcactccg tttgaccgtg aagacattta
    17461 cgagctctgc agccgcctgg acgacgtgat cgatctgatc gacgccgccg ccagccgttt
    17521 tgtgctctat cgcgtcgata gcgtacgcaa cggcaccatc gatctcgtta aggtcctggt
    17581 ttcggccacc gcggaggtcg aggtggccgt tcgcgccctg gaaacgccgc aaaaggccct
    17641 gaagcactgc atcgaaatca atcgctacga aaacgatagc gaccgcctgt gccgcacgct
    17701 catcgcgcag ctcttcgatg aagagaaaga ccccgtccaa attatcaagt ggaaagaaat
    17761 cttcgaagtc attgaaaccg ccgtcgacaa atgcgaagac gtcgccaacg tcatcgaagg
    17821 cgtcatcctc aagagcgcct gatccaccca tgccccgatt cgattcagca tctgccgtag
    17881 cgaaaaactg ccatccctgc caaagggaag gctcgctcgt cacttcctcc tcccttattt
    17941 ccttccctct cgaggcctcc gcccatgtct agcttctccc tcctcttcgt cgttatcttc
    18001 ctcaccctcg ctttcgaatt ctcgaacggc tggcacgacg ccgccaattc cattgccacg
    18061 gtggtctcca cgcgcgtgct cacgccgttc cgcgccgtcg tctgggccgc cttctggaac
    18121 ttcatcgccg ccttcgtttt cggcactgcg gtcgccaaga ccattggcaa gggcatggtc
    18181 catatcgaaa tggtggatga gaaggtcctc ctcgccgggc tcctcggcgc catcacctgg
    18241 aacaccatta ccctgatcct cggcctgccg acgagttcct ctcacgccct catgggtggc
    18301 tacggcggcg ccgcagttgc tcacgccggt ctcaaggccc tcgttctagg cggctggaca
    18361 aaaccggttc tttttatctt tctctcgccg attattggct tcttcgtcgc cgtgggcgtc
    18421 accattctca ccagttggat tgtccgccgc cagcgtcccc tgaaagtgga ccgctggttc
    18481 cgccgcttgc agcttctttc ggcagcgggt tatagcttgg gccacggcac caacgacgcc
    18541 cagaaaggca tgggcatcat caccactgcc ctcgtcgccg gcggcctaat gaagacctac
    18601 gacgtcccct actgggtcat catctgctgc cacctcgcca tggccggcgg cacgatggct
    18661 ggcggctggc gcatcatcaa aactatgggc cagcgcatca ctaagctcac ccccttcggc
    18721 gggtttgctg ccgagaccgc gggagcagtg acgctggtcg cgacggcgca ctttggcatc
    18781 cccgtcagca ccacccacac catcaccggc gccatcgtcg gcgtcggggc cacccgccgt
    18841 ctttccgctg tccgctgggg agtcacacga cgcatcgtgt tcgcctgggt tctcaccatt
    18901 cccggtgccg ccctgttcgg cgccgccctc ttccaactcg tccaccactg gctccgctag
    18961 taactcccgc gcgttcgtac gacctgtctc gcgattgcag atgcggggag catccccccc
    19021 gggaacgcca atctcccgat tggcgtcaat gttcgttgac accaaacttg cacataccag
    19081 ggaacgggcc gagcctgccg aagcaggctt ggttatcctg agatattgcg aatggcccca
    19141 cccgcccatc tcgcgcattt caccgaacgc cagcgaatgg attttcaatc aagattcctg
    19201 caatcctctg ccccgcgttt agatcctcag aaaggatccg ccccgccccg ctcttaaccg
    19261 ccgaggacac gatgagtgca tcccaaaatc cgacccgcga atcgtcctcg attcgaaacg
    19321 ccgctgccat ctccgccgga gtcgtttcca tgcaccaggc tgtgtaactg ctaacgacgg
    19381 cccgggccaa atccttgggc aggggtgtcg cgatctttcg cgtgacgtta acgtaaaatt
    19441 cctgcagcac ttgcatgctc agaatccctg tatgttcgct ccaaagctcg cgaagaacgc
    19501 tcttggccac ttcgtgtttc gccttcgcgt ctgcgtcgtg agcgtaaatc aaaatgttcg
    19561 tatccacgaa cgtcttagcg ctcatgccat tcgtcccgct tcgcccggat taccccaccc
    19621 aagcgaaagc ccgtctccag tagcgctagc gcctgccgct ccgcgcgttc gtaggcttct
    19681 tcctcgccca ccagcatctc aatctgctgc gcgagcagcc cgctgatgga agtgtctcgt
    19741 cgcgccgcca taatcttcgc cttccgaatg gtttgccggt ccaaactaat cgttacgttc
    19801 tgtttcaccc tgtcccccaa tcgcgaatcg acaccgctac catttcgcgg cacgtatatt
    19861 acgtgtaaca cgtaaaatac gtgctgtcaa tgatgctccg ctattcctaa cggagctagc
    19921 tggaacagtc cacctcccgc agggtttcga catcaatcaa ggcgggtcga atacgcgctc
    19981 accgaactgc tcctcgcctt ccgcacactt tgtcgttctc gctcgcctga tcaacttttt
    20041 ctccggaccc ggaagcccca gaaactttcc gtctcaatga aaagagggcg catcagcttg
    20101 acggctgacg cgccctcaat taattccccg cggcggttgc tctatttggc cagttgatac
    20161 tcaccctcta tatcgcacga aacgctgtca cccgacgccg cgcaggtgta cgtgcctttc
    20221 cccttgatgc ccttcagctt tccggagccg cccgtataca tccaggtgcc cttgccgctt
    20281 tgcagagcgc catctttcaa tgttcctgtt ccttgatacg acacgaaata cttgtcgcca
    20341 ctctccatgg ttgtcacgtg catcccgcgg gagcgaatgt tgttgccgct gatgtccatg
    20401 ctttccgtgc tcacgccctc ctttgtttta tcgcctccga tttcctgggg cttcgtccag
    20461 gtacatttcc tttgaccgat gcccaggctg tgatcggcac gatcccctgc aggaagcatc
    20521 tgttgccctt cggctttgcc aaactgagcg gttcccgtaa ctttggtttg cgcgcttgct
    20581 gtcgctgctg caaaacacac aattccacac atcacgaaca atcgacgcat aaaagtctct
    20641 ctcctttgaa atctttgaaa ttctcgcggg ccctggtgga ataacttgca ggcagcctag
    20701 cccttgcttc ccggagtgtc aagccgcttc cgctgatcgc ccacccgtca ttcgcgcatt
    20761 tcggcgcgtg ggtcaggctt tagaatggag gagttcaccg cttgaaattt ggtttttcaa
    20821 aagccggtta aaggccaatt ttggctattt ccagaaccgt cgagccctgg cctcatcaca
    20881 cggaatgttc gttctgcgtt tatccgcctc ttgaacccaa aagtatccgc cgcacgtcct
    20941 tcagcaacac gaagagatgc atcgggtctg taggcgacgg gataaaatcg aaatgttcgt
    21001 agaagcgcct tgcttcttca tcttttgcat gcacggcaaa ggcgcggatt ccggctatgt
    21061 ccgccgcttg cagcgtacgc tgcatcgcgt ctcgcaagag cgccttgcca acgccttgcc
    21121 cttgccaccg atgatcgatg gccagccgcg cgagcagcat gatggggacg ggatgctttg
    21181 ccagcccctt ctttaagcgc tcgggggaat cttccaacgt cacctggccc acggccaagg
    21241 tgtggtatcc aatgatggcc tcacccgcga cacccacgta ggtttgggcg gcgcctgcct
    21301 gttggttttg
//
LOCUS       DASYVO010000390         4081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_6697, whole genome shotgun sequence.
ACCESSION   DASYVO010000390 DASYVO010000000
VERSION     DASYVO010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6697"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VGR03_13150"
     CDS             <1..325
                     /locus_tag="VGR03_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995270.1"
                     /translation="EGPVGRLEGDLLHYTLRSVEEHAAKVENYTTRIAQDMYAEGRRS
                     WRAAMWLAAPWSWFQNYVLYLGFLDGYRGWVISRMAARGTWLKFKKLGKLVEAERRAG
                     GARKP"
     gene            322..1413
                     /locus_tag="VGR03_13155"
     CDS             322..1413
                     /locus_tag="VGR03_13155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1995271.1"
                     /translation="MRVLYVDLEREWRGGQSQALLTLVGLRERGHEVELLAAQDSPLA
                     KRVSEAGIKVHQVPRFGLRAWAARAIHSLITRKRFDLVHLNEPHALTAAWLAKAHRRL
                     PLLLSRRIGFPLQKNAVSQARYRAVERFVANSKDVAQSLVDSGIAAERISIVNEGVEI
                     FPARTPEERSKARKHWGVRENEFLFGCVSVFVPEKGQRHLIEALPLVRALHPEVRLLL
                     AGDGPCRAELRALTKRLGQTEAVLFPGFIKEVAQVYAALDAFVFPSEFEGLGTALQAA
                     MAAGLPCISTRRGALAEVVESERTALVAEPNGKEFAAMMLRLINDKELRKNLSKAGRR
                     EVEQRFSAGRMVEKTIHVYQDVLKNRRRA"
     gene            1546..3189
                     /locus_tag="VGR03_13160"
     CDS             1546..3189
                     /locus_tag="VGR03_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995272.1"
                     /translation="MRPLLAFLIMALLGSASAVAQYGKSTEPPLDLALTAVGRQHHAI
                     LTKSSEAQDYFDQGITLFYGFNHEGAARAFRRASELDPASPMPLWGIALAAGPNYNLD
                     VDAEREKLAYETVQKAEKLTEHAPQIEKDYVAALAKRYSGDASPNYRKLSLDFVAAMK
                     ALSQKYPDDLDAATLYAESLMNLNPWKLWSLDGKPAEGTDEIVRVLESVLQRNPNHAG
                     ANHYYIHALEASPYAERALPSAARLGGMVPWAGHLVHMPAHIYIRTGNYAEAARNNAE
                     AAQVDEAYLRQGHLMGSIYDLLYYSHNLHFLSAAQAMAGNFAAAKKAADELEAHIRPL
                     VGRMPEAESYLPTPILVLVRFHCWDEILNSSPPEPNLVMTSALWHFARGSAAAATGKI
                     EVAEVERKLLERVHGETSPEQEFGMNYNKARDFLDLAASILDARIATARSESGRAIAF
                     WIKAVQLEDRLRYNEPPEWFYPVRESLGAALLSAGKATEAEKVFREDLLQNPRNPRSL
                     FGLMSSLRAQHREEDAEWVERALKEAWKGADTKLNLSDL"
     gene            complement(3703..>4081)
                     /locus_tag="VGR03_13165"
     CDS             complement(3703..>4081)
                     /locus_tag="VGR03_13165"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEV1995273.1"
                     /translation="MDGLKIINDQYGHLTGSRALVRIGKILRNHSRAIDTAARYGGDE
                     FALVLPEAGKDIASRVVSRIRERLSAEPEHPALSVSAGVAAFPGDGDSPEKLLGAADR
                     ALYAMKHHGRSSVQNLTRIAACL"
BASE COUNT          917 a         1120 c         1259 g          785 t
ORIGIN      
        1 tgaggggccg gtaggacgcc tcgaaggcga tcttctccac tacacgttgc gctcggtcga
       61 agagcacgct gcaaaagtgg agaactacac gacgcggatc gcacaagaca tgtatgcgga
      121 gggacgccga agctggcgcg ccgcgatgtg gctggcggcg ccctggagct ggtttcagaa
      181 ctacgtgctg tatctcgggt ttctcgacgg ctatcgcggc tgggtcatct cgcggatggc
      241 agcgcgcggg acgtggctga aattcaagaa actcgggaag ctcgtcgagg cggagcgtcg
      301 cgcgggcgga gctcggaagc catgagggtg ctctacgtgg atctggagcg ggaatggcgc
      361 ggcgggcaga gccaggcatt gctgacactc gttggattgc gcgagcgagg acatgaggtc
      421 gagctgctgg ccgcgcagga ttcgccgctt gcgaagcgcg tttccgaagc tgggattaag
      481 gttcatcaag taccgcgatt cggcttgcgc gcttgggctg ctcgcgcaat acacagtttg
      541 atcacccgga agcgattcga ccttgttcat ctgaatgagc cgcacgcgtt gaccgcggca
      601 tggctggcga aggcgcacag gcggcttcct ctgctgctct cccgccgaat cggatttcca
      661 ctgcagaaaa acgcggtgtc gcaagcacga taccgggccg tggaacggtt cgtggcgaac
      721 agcaaggatg tggcacaaag cctggtcgat tcaggcatcg cagcagagcg catttcgatc
      781 gtgaatgaag gggtggaaat ctttcccgcg cgaacgccgg aagaacgaag caaagcgcgg
      841 aagcactggg gggtgcgaga aaacgaattc ctgtttgggt gcgtcagcgt ttttgtaccg
      901 gaaaaaggac aacggcacct gatcgaagca ctccccctgg tgagagcgct gcatccggaa
      961 gtgcgcctgc tgctcgcggg ggacggcccc tgccgcgcgg aactgcgggc gcttacaaag
     1021 cgtctcggac agacagaggc ggtgcttttc ccgggcttca taaaggaagt cgcgcaagtt
     1081 tacgcggcgc tcgatgcgtt tgtgtttccc tcggagttcg agggcctggg aaccgcgttg
     1141 caagcggcga tggctgcagg actgccatgc atttcgacga ggcgtggggc gttagcggaa
     1201 gtggttgaaa gcgaacgaac cgcactggtc gcggaaccca atggcaagga gtttgccgcg
     1261 atgatgttgc gactgatcaa tgataaagag cttcgaaaga atctcagcaa ggcgggccgc
     1321 cgcgaggtgg aacaacggtt ttctgccggg cgcatggtcg aaaaaacgat tcacgtatac
     1381 caggatgtgc tcaagaatag acgaagagct taaggctgtt ggaacactca ctgattcaaa
     1441 gcgtagtcta gagtgcagaa tcgagctgag atatactcca cccagctcga ccgttaccca
     1501 gacaggattc cggacccatc ctagattcct tcgtgcgagg cacccatgcg tccactactt
     1561 gcatttctga tcatggcact cctcggttcg gcgagcgcgg tagcacaata cggaaaatca
     1621 acggaacctc cactggatct tgcgctgacg gcggtcgggc gccaacatca cgcgattttg
     1681 acgaaaagta gcgaggcgca agactatttc gatcaaggga tcacactttt ctatggattc
     1741 aaccatgaag gagcagcacg ggccttccgg cgagcatcgg aactcgatcc tgcttcgccg
     1801 atgccgcttt ggggcatcgc cctggccgcg ggccccaact acaacttgga tgtagacgcg
     1861 gagcgcgaaa aactcgcgta cgaaacagta caaaaggcag agaaactgac ggagcacgcg
     1921 ccacagatcg agaaggacta cgtagccgcc cttgcgaagc gctactcagg agatgcatca
     1981 ccgaattaca ggaaactctc tctggatttc gtggcagcca tgaaggccct ctcgcagaaa
     2041 tatcctgacg acttggacgc ggcaacactc tatgcagaga gcctgatgaa tttgaatcct
     2101 tggaagctat ggtcgctgga cggcaaaccg gcggagggaa ctgacgagat tgtgcgcgtt
     2161 ctggagtccg tgctgcagcg taacccgaac cacgcaggag ccaatcacta ctacattcat
     2221 gctcttgagg cgtctcccta tgcggaacgc gctctaccaa gcgcggcaag gctaggaggg
     2281 atggtgccct gggccggtca cttggtgcat atgccggcac acatttacat ccgcacggga
     2341 aattatgccg aggccgcgag aaacaacgcg gaggcagcgc aggtggatga agcctatttg
     2401 cggcagggcc atctgatggg cagcatctac gaccttctgt actacagcca caacctccac
     2461 ttcctgtcgg cggcccaggc catggccgga aatttcgccg ccgcgaagaa ggctgccgac
     2521 gagctggaag ctcacatccg gccgcttgtt ggcaggatgc cagaggcgga aagttatctg
     2581 ccgacgccaa ttctggttct cgtgcgcttt cactgttggg atgaaatctt gaattccagc
     2641 ccgccggaac caaatctagt tatgaccagc gcactgtggc attttgcccg cggaagcgct
     2701 gcggcggcca cgggaaagat tgaagtggca gaagttgaac gaaaactgct ggagagggtg
     2761 cacggagaga cttcgccgga gcaagagttc gggatgaact acaacaaggc tcgagacttc
     2821 ctagatctag ccgcaagcat tctggatgcc cggatcgcca cggcgcgcag cgaaagcggg
     2881 cgtgccatcg cgttctggat aaaggcggtt caactcgagg acagactccg atataacgag
     2941 ccgcctgaat ggttttatcc cgttcgggaa tcactgggcg cggcgctctt gtccgcggga
     3001 aaagcaacag aggctgagaa ggtgttccgt gaagatctgc tgcagaatcc gcggaatcca
     3061 cgttcgctgt ttggcctgat gagcagcctg agggcccagc atcgggagga agatgccgaa
     3121 tgggtagaac gcgcattgaa ggaggcgtgg aagggcgccg atacaaagct gaatttgtcc
     3181 gacctgtgag cgctgcagag tccgaggcgc cagagggttc tggctcaaac ggatgaccga
     3241 aaagctgaaa cgcctggggg ttcctcacgg atccaccaag cgtttcaggc cttcttaaaa
     3301 ctcttgcgca agttaactgt cttctctttt tccagcgttt ccctcctctc cgctcgccgt
     3361 ttaggtcaag ctatcttggg gattgaacaa cccatcgtcc cttgggctcg aggaactcca
     3421 cgccaatagc aaagccgtcg ccttgcaatg gttggcagta agcaacccgg gccgaagagc
     3481 gaaactctcc agtgcgcgag gcaaatacca agggttcgcc ttgttcccag cggcgcacac
     3541 ttacgacgcg cgcgccacgc gcactcacat tctcagtaaa ggtcgattcc gaaccgggtg
     3601 cctggcgatg gccgtcaatc aaaacggaga tttccatcgg gatgcgattt tcgactcggt
     3661 gaaatgaaaa cttctttccg cgaggcatgt tcttgttcat cctcacaggc acgccgcaat
     3721 gcgcgtgaga ttttgaacgc tgctcctgcc atggtgcttc atggcataga gcgcgcggtc
     3781 ggcagcgcca agaagtttct ctggcgagtc gccatccccc gggaaagccg cgactcccgc
     3841 actaacggaa agcgcagggt gctcaggctc agcggagaga cgctcgcgga ttcgagagac
     3901 gacgcgcgac gcgatatcct tgccggcttc cggcaatacc agcgcgaatt catcgccgcc
     3961 gtatcgcgcg gcggtatcaa ttgcgcggga atgattgcgc agaatcttgc ctatacgtac
     4021 cagcgcgcgg ctgccagtca gatgcccgta ttgatcgttg atgatcttga ggccatccat
     4081 g
//
LOCUS       DASYVO010000391         4030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_80307, whole genome shotgun sequence.
ACCESSION   DASYVO010000391 DASYVO010000000
VERSION     DASYVO010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80307"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VGR03_13170"
     CDS             <1..299
                     /locus_tag="VGR03_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995274.1"
                     /translation="RNIVKAIHSSPTWHDGRNAIVVLWDEDDYSVSPTTNKVLLIVDT
                     NYGADGVKDANFYTHFSLLKSIEGGFRLPCLNHACDATTNVIASLFRGGDDDDR"
     gene            452..1354
                     /locus_tag="VGR03_13175"
     CDS             452..1354
                     /locus_tag="VGR03_13175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEV1995275.1"
                     /translation="MKHGKLLKEVSRVIEPYCITKGEKFRLKDYDPADTNGLKDKHQA
                     QGLLEDGIALLSHMQEKLYAQDRWALLLIFQAMDAAGKDGAIKHVMSGVNPQGCDVSS
                     FKSPSSEELNHDYLWRTHKCIPERGKIGIFNRSYYEEVLVVRVHPPFLRAQKLPDKLI
                     TKHIWDERYEDINSFERYLTRNGVVIRKFFLHVSKKEQKKRFLERLEDSKKNWKFSMA
                     DVQERGFWKDYQEAYEEMIQRTATKHAPWYVIPADNKWFTRLAVASAIIETLDQLDLA
                     FPDVDEAKKKELEAVRTSLLSQKD"
     gene            complement(1802..2089)
                     /locus_tag="VGR03_13180"
     CDS             complement(1802..2089)
                     /locus_tag="VGR03_13180"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEV1995276.1"
                     /translation="MVIHTFAFRWKAGVREDQKVRAVAEIRGLQGQIPGLLETWVGVN
                     VSPRSQGYELGGVMKFADRCALDAYGGHPVHQKLLQWLMPLIDPIEIDFET"
     gene            complement(2229..>4030)
                     /locus_tag="VGR03_13185"
     CDS             complement(2229..>4030)
                     /locus_tag="VGR03_13185"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEV1995277.1"
                     /translation="VAAGESSSRGELRLLRNLGSKGWADVTKDVHLDAVKLNQPRAIA
                     VADIDGNGDADLVLTQLGGAPLVLRNEGGNKNNWMSIDLKALNDNKSGIGTKVELYAG
                     TLYQKWEVAGASGYLGQSAAPILAGLGSERNAEVVRLLWPMGVPQDEINLAAKKVQSI
                     TELDRRGSSCPVLFSWNGREYEFIADMIGPGVVGHWVAPGERDVPDPDEYLKVPARSV
                     KARNGLLSFRFMEPMEETVYLDQVRLVAIDHPAAYEVFPNERFVSAPPFPEFRVIASR
                     DAHAPIGAWDDRGNDVLRLISTRDRQYVTSFEELPFAGFAKLHWIELDLGEWDAQKPL
                     RLIIDGYTDYFTATSMYAADQAGIKVIPPYVEALDAQGKWVRVVEDMGFPAGLERTMV
                     ADLTGKLPAGTRRIRIVNNLKIYWDAIRIDQTPDVRDVRVAQVPLAKAALEFVGYPRE
                     TRLKPASDTIYSYSHRSVTGPYAHAAGNYTRYGDVYGLLSASDDRFVVFGSGEGLKLD
                     FDPRQLPALPAGWVRDYFFYADGFEKDLDFYAAHAFTVEPLPRHSLLPYPYPEGRDYP
                     ADAEHLDYQLEYNTRPRSERLPPSLRYQYSSPH"
BASE COUNT          884 a         1297 c         1049 g          800 t
ORIGIN      
        1 tgcggaacat cgtgaaggcg attcatagct ctcccacttg gcacgacggc cgcaatgcca
       61 tcgtggttct ctgggacgaa gacgactaca gcgtgtctcc gaccaccaac aaagttctgc
      121 tgatcgtcga taccaactac ggtgcggacg gcgtgaagga cgcaaacttc tacacgcact
      181 tctcgctgct gaagtccatc gagggcggct tccggctgcc ctgcctgaac cacgcctgcg
      241 atgccaccac caacgtgatc gccagcctgt tccgcggcgg cgatgacgac gaccgctagg
      301 atcgctgaaa cttgaacggg cgggctcgct ccgcgatttg cggggccgag cccgccgtgc
      361 tttctctatg caagggccgc catgcgctcc ccttgaaaag tatcccggtc cgctgttctg
      421 agccgcacgt ctgtactaca ctgccggaat catgaagcac ggcaagctcc ttaaagaagt
      481 ttcgcgcgtc atcgaaccct actgcatcac caagggcgag aaattccggc tgaaggacta
      541 cgaccccgcc gataccaacg gcctcaaaga caagcaccag gcccagggcc tgctcgaaga
      601 cgggatcgcg ctcctcagtc atatgcagga aaagctctac gcgcaagatc gctgggctct
      661 cctgctgatt tttcaggcca tggatgccgc tgggaaagac ggcgccatca agcacgtgat
      721 gtccggcgtg aaccctcagg gatgcgatgt ctcttccttc aagtcaccca gcagcgagga
      781 actgaaccac gattatctct ggcgaacgca taagtgcatc ccggagcggg gcaaaatcgg
      841 catcttcaat cgctcttatt acgaagaagt tctcgtcgtg cgcgtccatc caccgtttct
      901 gagggcccag aagctgccgg acaagctgat caccaagcac atttgggacg agcgctatga
      961 ggacatcaac tccttcgaac ggtacctcac gcgcaacggc gtcgtgattc gcaaattctt
     1021 cctccacgtt tcaaaaaaag agcagaaaaa gagattcctc gagcgcctcg aagattcgaa
     1081 aaagaactgg aagttctcca tggccgacgt ccaggagcgc ggcttttgga aagattatca
     1141 ggaagcctac gaagagatga ttcagcgcac cgccacgaag catgcgcctt ggtacgtcat
     1201 cccggcggac aacaagtggt ttacgcgcct ggccgtcgcc tcggcgatca tcgaaacgct
     1261 ggaccagctc gatctcgcct tccccgacgt cgacgaagcc aagaagaaag aattggaggc
     1321 tgtccgcacc tctcttcttt cccagaaaga ttagaagttc gtttcacgcg gatacgcccg
     1381 cggtagcttt tgtagaggcg gctttatgcc gccatcttcc gatcgtcgtc aggcttgcct
     1441 cctgcgcgtc attcttttgt aggggccgcc ttccgaggcg gcccgctctc ctttttccag
     1501 tgggcgcctt tctactcttt ccgcaacctc ttaacagtcg attcccgctc cgcaaccccg
     1561 aacaaaaatt tggagcgggc tacccaccct ctcccgaggc caggcgtccc gctccaccac
     1621 agaacgaagg aattgccgca aagcggctgc cacttcctcc ctgtctgtgc aaaaactcaa
     1681 ctccttgcat caaaacgaat tacaaatccc ccgccgatcc cgcgcaaacc aatccccact
     1741 aattatcgaa acctatggag actccatatg gaaatccacc gggaccgccg cgcggtccac
     1801 atcacgtttc aaaatcaatt tcgatcggat caatcagcgg catcagccat tggagcaact
     1861 tctgatgaac aggatgccct ccgtaagcgt ccaacgcgca ccggtcggcg aacttcatca
     1921 ccccgccaag ctcatatccc tgcgagcggg gtgacacatt gacaccaacc caggtctcca
     1981 gcaggcctgg aatctgcccc tgcagtccac gaatttccgc aaccgcgcga accttctgat
     2041 cctcgcgaac gcccgccttc caccgaaaag caaatgtatg aataaccatg catgcattat
     2101 aacgaacgtg cggcggccct tcccggaatt tgcaaacggg aagcacgagt tgctgttctg
     2161 caaagccagc gcaaaaagcg cgtgggatcg cgcatgggta cccgcgcttt ggtactgagt
     2221 gagccgcttc agtgcggcga agaatactgg tagcgcaggc tcggcggcaa acgttccgaa
     2281 cgtggccgcg tgttgtactc cagttggtag tccagatgtt ccgcatcggc cggataatct
     2341 cgaccctcag gatacggata cggcagcagc gaatggcgcg gcagcggctc gacggtaaac
     2401 gcgtgcgccg cgtagaaatc gagatccttc tcaaacccgt cggcgtaaaa gaaataatcg
     2461 cgaacccatc cggccggcag cgctggaagt tgccgcggat caaaatcaag tttcagtccc
     2521 tcgccggaac caaaaacgac gaagcgatcg tcggaagcgc tcagcaagcc atacacatcg
     2581 ccgtagcgcg tgtaatttcc cgccgcgtgc gcgtaaggac cggtcacgct tcggtgcgaa
     2641 tacgagtaga tcgtgtcgct cgccggcttc agccgcgtct cgcgcggata gccgacgaat
     2701 tccaacgccg cctttgcgag aggcacttgc gccacgcgca cgtctcgcac gtccggcgtc
     2761 tgatcgatcc gaatcgcgtc ccaataaatc ttcaaattgt tgacgatgcg aatccgccgc
     2821 gtgcccgcgg gaagtttgcc ggtcaagtcc gcaaccatgg tccgttcaag tcccgcagga
     2881 aaacccatat cttccacgac acgcacccac ttgccttgcg catcgagggc ttctacatac
     2941 ggcggaatca ctttgattcc cgcttgatcc gccgcgtaca tcgaagtcgc ggtgaagtaa
     3001 tccgtgtaac catcaatgat caatcgcaac ggtttttgcg catcccattc accgaggtcg
     3061 agttctatcc agtgcagctt cgcgaatccc gcgaacggca actcctcgaa actcgtgacg
     3121 tactggcggt cgcgcgtaga gatcagccgc agcacatcat ttccgcggtc gtcccacgcg
     3181 ccgataggcg catgcgcgtc gcggctcgcg atcacacgaa actccggaaa aggcggcgcg
     3241 ctgacgaatc gctcattcgg aaaaacttcg tatgccgccg gatgatcgat ggccaccagc
     3301 cgtacctgat cgagatatac cgtctcttcc atcggctcca tgaagcggaa actgagcagg
     3361 ccgttccgag ccttcacact gcgcgcgggc accttcaagt actcgtccgg atcgggaacg
     3421 tctcgttccc cgggcgcaac ccagtggccg accacgccgg gcccgatcat gtcggcgatg
     3481 aattcatact cgcggccatt ccacgaaaac aaaacggggc acgagctccc gcgccgatcg
     3541 agttcggtga tgctttgcac ttttttcgcg gccaggttta tttcgtcctg cggcacgccc
     3601 atcggccaca gcaatcgaac gacttcggcg ttgcgctccg agccgaggcc ggcaagaatc
     3661 ggtgcggcgc tctgccccaa atagcccgac gcgccggcaa cttcccactt ttgatacagc
     3721 gtgcctgcgt agagctcgac cttcgtcccg attccgctct tgttgtcgtt gagcgccttg
     3781 aggtcgatgc tcatccagtt gtttttgttg ccaccctcat tgcgcagaac cagcggcgcg
     3841 cccccgagtt gcgtcaggac caaatccgcg tcgccatttc catcgatatc cgcaaccgca
     3901 atcgcacgcg gttgattcag cttgaccgca tcgaggtgta cgtcctttgt gacgtcggcc
     3961 cagcccttgc ttcccagatt tcgcaacaac cggagttcgc ctctgctgct cgattctccc
     4021 gcggcgacaa
//
LOCUS       DASYVO010000392        14775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_39517, whole genome shotgun sequence.
ACCESSION   DASYVO010000392 DASYVO010000000
VERSION     DASYVO010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 14775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14775
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39517"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..285)
                     /locus_tag="VGR03_13190"
     CDS             complement(31..285)
                     /locus_tag="VGR03_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995278.1"
                     /translation="MSATVKRLSWFILKSRERALSVSEYSTLRGVDFQGISVYRSDED
                     LEAAQGTGSGAAIYRLDEPTTSIGLNFRMRRNPGEIQKQS"
     gene            complement(331..1305)
                     /locus_tag="VGR03_13195"
     CDS             complement(331..1305)
                     /locus_tag="VGR03_13195"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEV1995279.1"
                     /translation="MKAAVYSTYGPPDVLQIRDVEKPVPRDNEVLIKVRAASANPLDW
                     HFMRGTPYFVRILVGLRKPKVTRLGVDLAGQVEAVGRNVTQFKPGDEVFGACRGAFAE
                     YVCAVEDKLALKPANITFEDAAAVPVVAITALQGLRDRGRIQRGHKVLVDGASGGVGT
                     FAVQIAKSFAAEVTAVCSTRNVDTARSIGADHVIDYTRQDFTQSGRRYDLILAANAHH
                     SIFDYRRALSQDGIYVMAGGGGAQILQVMLLGPLLSLIGSKKMCFVMAKINQKDLVFL
                     KDLLETGKVVPVIDRRYSFSGVAEALRYLEEGHAQGKVVITVEHGNDT"
     gene            complement(1408..2106)
                     /locus_tag="VGR03_13200"
     CDS             complement(1408..2106)
                     /locus_tag="VGR03_13200"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEV1995280.1"
                     /translation="MHPTNRTARVAGLLYVLMGLLVPFSLIYIPRTLIVPGNATATAN
                     NILGHEMLFRAGIVTGLASAVAFVLLVIALYRLLSGVNKTYAALMVILVVVSVAIGFL
                     NEVNNIAALTLFRGADFLAVFDKPQRDALAMLFLGLHRQGLVVNEIFWGLWLFPFGVL
                     VMRSGFLPRILGVLLIVNCFAYVAASLISLLLPSYANVVFRAIQPALLGELWIMLWLL
                     IKGAKVQPSHAPAS"
     gene            complement(2178..2549)
                     /locus_tag="VGR03_13205"
     CDS             complement(2178..2549)
                     /locus_tag="VGR03_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995281.1"
                     /translation="MRSRLKRLIFIAPLAIVGMLLFIAIGGEIVLQLWNWLLPSLFGG
                     RQITFWQALGILALCRILFGGSGWHGSVRSNLRRRMEERCKNRMEERCEHMTPEERER
                     FRQRMRERFGFGPSTGESKEQ"
     gene            complement(2560..3141)
                     /locus_tag="VGR03_13210"
     CDS             complement(2560..3141)
                     /locus_tag="VGR03_13210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV1995282.1"
                     /translation="MDEPSSITSMTEQDRQISEIIAEERSRLRNFIRRRVRDPSDAED
                     IVQEVFYELVEANRLLMPIEHVTGWLFRVARNRITDLFRKKKTETFSDAAVEDEDGEV
                     LQIEDLLPSPDAGPEALYARRVLLNELELAVDGLPEEQREVFVGHELEGRSFKEMAAE
                     TGVSVNTLLSRKRYAVLHLRERLQSIYDEFTKG"
     gene            complement(3936..4679)
                     /locus_tag="VGR03_13215"
     CDS             complement(3936..4679)
                     /locus_tag="VGR03_13215"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1995283.1"
                     /translation="MGEKQNQPFQLSFNPSLKVDFQGSRVTSDSGLLLVRELDERLGL
                     SALILENIMDGRRGKNMPLPLPDLLRQSINSRLAGYEDVNDAERLSQDPTFRLIGSEK
                     IWERGAALPSRLHWFETEVLSQEGNLDGLSRINRELLAKAEAMDSLQRVVLDMDSTEI
                     PVYGEQEHSAYNGHFESTCYHPLLLFNGEGDCLGAKLRPGNVRSAEDWEEVLLPEIER
                     QQEVGKEVVFRADAAFAKPEIYEALGDRE"
     gene            4907..5365
                     /locus_tag="VGR03_13220"
     CDS             4907..5365
                     /locus_tag="VGR03_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV1995284.1"
                     /translation="MWKLIGALSALALFAVPASLQETKKPAEEKPAAEYKIPPEDAKK
                     ENPVKPNEESAARGKKMYGYECEMCHGKDADGKGDVAEDMKLKLADFRDPAALKERTD
                     GELFYIITKGKAQMPGEGDRMKPDQVWDMVNYIRSLAKKESTPKPKTEKP"
     gene            complement(5471..6352)
                     /locus_tag="VGR03_13225"
     CDS             complement(5471..6352)
                     /locus_tag="VGR03_13225"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEV1995285.1"
                     /translation="MADTAEQPKPAPPPSAHALQGEEFRAKLRSVARRQWWLWSSAIL
                     ITILLALTVASFAFPELLAHSESTYAFELGQAVRGLVGLVLLFNINAVYQQFQINRMQ
                     RHMSEQFDAMTRIEVRTEEVYKLAALDPLTGLYNRRSGEQRLAEEISRAQRHGRALTV
                     ILFDLNGLKFINDKFGHAAGDEAIKTLAARLARAIRGSDLAVRIGGDEFMVLLPECKP
                     EEVRHVLGRVKDLKIEVAGQVQEFSFAAGWTDYVRGELPEDLLKRADAALYVNKRAAK
                     SQRESAAVSPSSPAKPT"
     gene            complement(6634..7044)
                     /locus_tag="VGR03_13230"
     CDS             complement(6634..7044)
                     /locus_tag="VGR03_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995286.1"
                     /translation="MPDQIRQVDHFSASIPHKAGEGARVLGALRDAGINLIAFWGYPS
                     GAGRAQLEFIPKNGAAFAAAAKRVKLRVKKSSAFYISGGDRPGAIAGILARLAAARIN
                     VGALQAVSAGSGHYGAVLFLSAGATRKAARVLGL"
     gene            7440..8558
                     /locus_tag="VGR03_13235"
     CDS             7440..8558
                     /locus_tag="VGR03_13235"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEV1995287.1"
                     /translation="MAWIVLSGLMGGCSGGGSITSGNGGGPVPEVAFAIDGNANTVSV
                     FTVDTTIGTWTPIGTIATGTKPLAVALHPSGKFLYVANFNSANISAYNIDPTTGALTA
                     MMGSPFAAVNAPQALAIDSSGNFLYVSTLTAAMPPVHVLSGYSINATTGALTLIAQPN
                     VPADTTPVGLTFAPTTEFLYAADMNNNVLGFSAHAATGVLAPVPGNPFAAGTMPFGIV
                     AHPTGKFVYVTNIISGDVSGYLVDALTGRLTPVPMSPFTSGTSPGPIPATIAVEPTGK
                     FLYVVNAGEADISEYSIDATSGALAPLTTSPFVLPAGTILLRMAVDPTGTFLYVVNFS
                     GNNISIFMIQSNGSLNPMGTTLMGAGATSGIAFRAGHP"
     gene            complement(8708..9217)
                     /locus_tag="VGR03_13240"
     CDS             complement(8708..9217)
                     /locus_tag="VGR03_13240"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016102133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV1995288.1"
                     /translation="MAASLFAASNLYDYTLPSIDGKPMPLANFKGKVLLLVNVASRCG
                     YTPQYTALEATYEKYKDRGLVVLGFPANNFGAQEPGTNKEIKTFCSTKYSVKFPLYAK
                     ISVKGGDQAPLYQFLTSKDSNPAVAGDIKWNFTKFLVDRNGKVVQRFEPDVTPDSPEV
                     IAAIEQLLK"
     gene            complement(9448..9825)
                     /locus_tag="VGR03_13245"
     CDS             complement(9448..9825)
                     /locus_tag="VGR03_13245"
                     /inference="COORDINATES: protein motif:HMM:NF025279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HEV1995289.1"
                     /translation="MGQNESAFRARGARLAAIGLGDFRYARIFREETGITFPLLIDAN
                     REAYRAAGLGSANVLHLLRRDNAAARKHARAAGFRQVKLGKNPFQLGGSFVFGPGNRD
                     LFAHISRTFSDNASPADLLAKLP"
     gene            complement(10250..10774)
                     /locus_tag="VGR03_13250"
     CDS             complement(10250..10774)
                     /locus_tag="VGR03_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995290.1"
                     /translation="MKLTLRGLLIAGLHVALVCSLGAKLLYDRATRPRVWVRAAPYDP
                     DLPIRGRYVSLRLQMSGIGFAVPESRNTEGRAPARVYANARLEVQNGQLVAVYSDRTS
                     DPAVWEVFRDSVPTISLFEPVAFFIPEHIQDPSRRPAGEELWVEVTLPRKGPPRPIRL
                     AVIKNGVFTPLDIR"
     gene            complement(10771..11931)
                     /locus_tag="VGR03_13255"
     CDS             complement(10771..11931)
                     /locus_tag="VGR03_13255"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HEV1995291.1"
                     /translation="MPANLEAQLQRWVSAGVVDAATADRLRDFEAQHSGSQKLRWPII
                     LALSFGGLLLAAGVLLFVAAHWDELSPSKRFALVLLLVALFHAAGAAAAGRFTALSRV
                     LHAVGTATLGAGIFLAGQIFNLEEHWPGGIMLWAIGAALAWWILRDWIQGLLVALLVP
                     AWLAGEWIVATENFVGGERILAEGLLLLAITYLTGSIAERRHPLRRALVVVGAIALIP
                     SVPFAIESASDFYRWRHEPVVPLGLAALGWTLAFGLPLLLAVFLRGRAAALNLLAALW
                     VWLLGTTGSNAEGGQLPILQFAWHELGPYFLCGLGSVGLIAWGLRESRKERINLGIAG
                     FALTVLVFYFSDVMDKLGRSEGLIGLGVLFLFVGWLLEKTRRRLVARLGGQA"
     gene            12211..>14775
                     /locus_tag="VGR03_13260"
     CDS             12211..>14775
                     /locus_tag="VGR03_13260"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEV1995292.1"
                     /translation="MSRKVTGAFAVMLGCGVALLGLLPKAPGRYARRTTLERSATRPG
                     VVDAGLEARVREALRSAPLAFEANLGQTDEQVKFTAGGAGYTLFLTGEEAVIALHRPF
                     AREKKPVYLEERAQGWHAGEPMSAVLRLKFIGASATTRLTGIDEQPARTNYFIGNDES
                     RWRRNVPNYREVRYENLYPGVDAVFYGDRRTLEYDFVVKPGADPRVITLGFEGARNLK
                     LSPAGELAAETPLGEVRLLQPRVYQETISGARVPVAGKYVQRGEGLIGFELAGYDAGR
                     AVVIDPPVSVMPRMLASPPPPPGPPTPTGSALQLGTYLGGQFDEAINAIAVGSNGQVY
                     VAGFSDSFSGFPAAGTPFQGFNAGAFDCSQFPQHPCGDAFVATLNTTTGALVATTFLG
                     GGADDVAWGLALDSANDPYVVGESDSGNFPTTAATAFQATPGNASCASGSRTRPCRHA
                     FFSKLTPNLSSLTYSTFLAGTDDDSAFAVAVDPSGNAFITGVAGFGFTTVTPASGAYS
                     GAGDAFVARVDPTASGTGSLIYATYIGGSGTDEGLAIAVDVNGNASIGGATFSTVNTS
                     VAFPTTINAVQAGSSDASFCGPGGGFNCGDGFIAQVDATGTLTYASFFGGDSTDQVNG
                     IALDAAGNIFVTGATSSHSIPIPTTPGAFDTAGSSGFDAFAAKINPAAAGLAGVTYST
                     FLAGSNDDSGNAIAIDPSGNAFIAGTTNSTDYPTFLALQAASRVGFFSQTSFVTSLNA
                     AGSALNFSTYYGGTNTETGRAVAIDAAGLIYAGGSTNSTDLCTAKAFQPQNNSQFGTA
                     DAFLTIINPTNTAAVCVMMTNGTMPPGPVDFGQVDVGTTSFPQTVDLFNAGSAAMTF"
BASE COUNT         3162 a         4199 c         4721 g         2693 t
ORIGIN      
        1 gagacgctcc acgctaggtt gctaaacagt tcaggactgt ttctggattt ctcccgggtt
       61 acggcgcatc cggaagttga gaccgatgga cgtggtgggc tcatcgagac gataaatagc
      121 agcgccggaa cctgttcctt gcgctgcttc caaatcctca tcggatcggt agacgctgat
      181 gccttgaaaa tccacgcccc gcaaggtgga atactcacta acggagagcg ccctctctct
      241 cgatttcagg atgaaccaac tcagacgctt aacggtcgcg ctcacccgcc ttgcgaagcg
      301 cagcggagca aagtcgggtg cagcgcgttg ttaagtgtcg ttaccatgct caacagttat
      361 tacgactttg ccttgcgcat gcccttcttc cagataccgg agagcttcag ccacgccgct
      421 gaacgagtaa cgtcgatcta tgacgggtac aacctttccg gtttcaagaa gatcttttag
      481 aaaaaccaaa tccttctggt ttatcttcgc cataacgaaa cacatcttct tgctcccaat
      541 cagtgacagt aatggtccca gtaacataac ttggagaatt tgagccccac cgcctccggc
      601 catgacataa attccgtcct gactcagcgc acgccggtaa tcgaagatcg aatgatgtgc
      661 gttggcggca agaatcagat cgtaacgccg cccactctgc gtgaaatctt gtcgagtgta
      721 atcaatgaca tggtctgcgc caatcgatcg cgccgtgtcc acattcctgg tgctgcacac
      781 ggcagtgact tccgccgcga acgatttggc aatctggacc gcaaatgtac ccacaccacc
      841 cgacgcgcca tccaccagaa ccttgtggcc tcgctggatt cgtccccgat cacgaaggcc
      901 ctgaagggca gtgattgcca ccacgggtac ggctgctgcg tcctcaaacg ttatgttggc
      961 cggcttcagc gccaatttat cttcaacagc acacacatac tcggcaaagg ccccgcggca
     1021 cgcgccgaac acctcgtcgc ctggtttgaa ctgggttacg ttcctgccaa ccgcttccac
     1081 ctgaccggcc agatccacgc ccagtcgtgt gacctttggt ttgcgcagcc cgaccaatat
     1141 gcgaacgaag tacggcgtgc ctctcatgaa gtgccagtca agtggattgg cggaagccgc
     1201 gcgaactttt atcaaaactt cgttgtccct gggaacaggc ttttcaacgt ccctgatttg
     1261 gagaacgtct gggggcccgt atgtgctgta gacggcggct ttcatcggcg ttcctccgcg
     1321 gcgctggcct gctgcttccc tattcgtttt tacctgcgct cttgtcatga tggcgttgct
     1381 ggcgtggcgc gcgctgcaac cgctcgatca agaggcggga gcgtgcgatg gttgcacctt
     1441 ggcgccctta atcagaagcc acagcatgat ccacaattcg ccaagtagcg ccggctggat
     1501 cgctcggaaa acgacattgg cgtagctcgg caagagcaac gatataaggc tggcggccac
     1561 ataagcaaaa cagttaacga tcagcaggac gccgaggatc cggggcagga agcccgatct
     1621 catcaccagg acaccgaacg ggaaaagcca cagcccccaa aagatctcgt tgaccacaag
     1681 tccctggcga tgcaagccga ggaagagcat ggctagagcg tcccgctgcg gtttgtcgaa
     1741 taccgcaagg aaatcggcgc cgcggaagag cgtgagtgcg gcgatgttgt tcacctcatt
     1801 caggaacccg atcgcgacgg agaccacgac caggatcacc atcagcgcag cgtacgtttt
     1861 gttcacgccg ctgagcaaac ggtagagagc gatcaccagc aagacaaaag cgaccgccga
     1921 ggccaggcca gtgacaatgc cggcacggaa aagcatctcg tggcccagaa tgttattcgc
     1981 cgtcgcggtg gcgttgcctg ggacgatcag agtgcgtgga atatagatga ggctgaaggg
     2041 gacaagtaat cccattaaca catacagtaa tccagcgact cgtgcggttc ggttggttgg
     2101 gtgcatcact ctgtctcctt gttcgctgtg ggtgaaccgc cctcaggtcc gctttgcatt
     2161 gggctgccga cgcggcttca ttgctccttg ctctcacccg tagatgggcc gaagccgaaa
     2221 cgttcgcgca tgcgttgccg gaatcgttcc cgctcttcag gcgtcatgtg ctcgcagcgc
     2281 tcttccatgc gatttttgca gcgctcttcc atgcgacggc ggagattgga gcgaacggaa
     2341 ccatgccatc cagatccacc gaaaaggatg cggcacagcg ccagaatccc gagcgcttgc
     2401 cagaaggtaa tctggcgccc gccgaagagc gagggcagca gccaattcca cagttgcagc
     2461 actatttcgc cgccgatggc gataaacagc agcatcccca caatcgccag cggagcgatg
     2521 aaaatcaatc tcttcaatct cgatctcatg ttcagctcct catcctttcg tgaattcgtc
     2581 gtaaatgctt tgcaatcgct cgcgtagatg caacaccgca tagcgtttgc gcgagagcag
     2641 cgtattcacg ctcacgccgg tctcggcagc catctcctta aagctgcgcc cttccaactc
     2701 gtgcccaaca aacacctcac gctgctcctc cggcaacccg tcgaccgcca gctcgagttc
     2761 attgagaagc acgcggcggg cgtagagcgc ttccggcccg gcatcgggcg atggcaaaag
     2821 atcttcgatc tgcagcacct cgccgtcttc gtcctcgacc gctgcgtcgc tgaaggtctc
     2881 tgtcttcttc ttgcggaaaa ggtccgtgat gcgattgcgc gcgacgcgaa aaagccagcc
     2941 ggtcacgtgc tcgatcggca tcagcaggcg attggcttct accagttcgt agaagacctc
     3001 ctgcacgatg tcctcggcgt cggagggatc gcgcacccgc cggcggatga aattgcgcag
     3061 ccgggatcgt tcttccgcga tgatttcgga gatttgacgg tcttgctcgg tcatcgaggt
     3121 gatgctcgac ggctcatcca tccattcctg tagacgaacg gcggccgcaa atattgtaga
     3181 gatttgaatc ggcgaaacgg ggtgggcgcc acaccacgag gctgaagaac gccgcgagca
     3241 aagagaggct cctgttcacc ggcttaaaca agtcatagaa gagtgccgtc gcagcgatag
     3301 agcacgcgac cacgatgagc tcggctgcga acccaaccgg aataaaggct cgtgcgccag
     3361 gatgttggtc gctgtagccg cggcgtcgcc agatacaaca agcctgccgc taaccaagac
     3421 ggcgactcct cccgtcagga aggtgagcaa ataaaagaca ccggcaattc tggtcttgag
     3481 acgcggcgac gcttctgcaa tccattccat cattttatag cccccaggag gggccgccgc
     3541 atgaacgtgg cgacgccgaa tattacgccc ataagggccg tgagctcaac gagaccgccc
     3601 gccatggcga accgcccact gggaccagcg ctttcctttg cgcgcaggta gagctcaaga
     3661 acggcgaggg gtagcaagta gcagccgaaa aaccaaaagt tttgcagtga atgccgcttt
     3721 tgcgatgcgg tgctgttttg caaatgtggc aggtcgcgcg gccggatgta agtcgcgtgc
     3781 aggagcacac ctccattgtt ggactacacg caccggggga gcggttagtt ccctagcgtg
     3841 catgggtttg tgactgggcc gccccaccgg gcgcgtcagc agttcctcga tgtcccgctc
     3901 cacagtggcg ttagacggaa gccgaatcgc gcacttcact cccggtctcc cagcgcttcg
     3961 tagatctccg gtttggcaaa cgcggcatcg gctcgaaaca ccacttcctt gcccacttcc
     4021 tgctggcgct cgatttccgg cagaagcact tcctcccagt cctcggcact gcgtacgttg
     4081 cccggtcgca acttcgcacc gagacagtct ccctccccgt tgaacagcaa cagcggatga
     4141 tagcaggtcg attcaaaatg cccgttgtag gcactgtgct cctgttctcc gtagactggg
     4201 atttcggtgc tgtccatatc cagcaccacc cgctggaggg aatccatggc ctccgccttc
     4261 gccagcagtt cccgattgat tcggctcaga ccgtccaggt tcccctcttg gctcagcacc
     4321 tcggtctcga accaatgcag gcgcgaaggc aacgctgccc cacgctccca gatcttctcg
     4381 gagccgatca agcggaaggt cggatcttgc gacagccgct ccgcatcgtt cacatcttcg
     4441 tatcctgcca agcgactgtt gatggactgt cgcagcagat ccgggagcgg caacggcatg
     4501 ttctttcccc gccgaccgtc catgatgttt tcgaggatga gcgcactcaa tcccaaacgt
     4561 tcatccaact cgcgcaccaa aagcaggcca ctgtcggagg tgacgcgaga accttggaaa
     4621 tcaactttca aagacgggtt aaacgagagc tgaaagggct gattttgttt ctcacccact
     4681 gggtcgaggc ctccacgaag tcgatttcac tttcaaagcc gcatgaatgc tggcgttggc
     4741 gccctcatca ggcgacttac gctagattca aaaaggaaat gtggattgaa agccgcgatc
     4801 cgaagagagc gtgcttgacg aatctggacc gcaggagaat ccgcttcccg ctcgacaccg
     4861 ccgggcgtag aatcagcgcg aagacatcgg cgagcgggag attgcaatgt ggaagctaat
     4921 aggagccctc tcggcactgg cactctttgc tgttcctgcc tctttgcagg aaaccaagaa
     4981 accagcagag gaaaagcccg ccgcggagta caagatccct ccggaagatg ccaagaaaga
     5041 gaaccccgtc aaaccaaacg aggaatccgc ggcccgcggg aagaagatgt atggctacga
     5101 gtgcgagatg tgccacggca aggacgccga cgggaaaggt gacgtggcgg aagatatgaa
     5161 gcttaagctc gcggattttc gcgaccccgc ggcgttgaaa gagaggacgg acggcgaact
     5221 cttctacatc atcaccaaag gcaaggccca gatgccgggc gagggagacc gcatgaagcc
     5281 ggaccaggtc tgggacatgg tcaattacat ccgctcgctt gccaaaaagg aatctacgcc
     5341 gaaaccaaaa acggagaaac catagagagt gccggctgcg ggaagtgggc agttgccagt
     5401 tgccagttgt caattttcag ttgtcagtcc ttagccctca gactttagtc ctcaggataa
     5461 atcaagactc ttaagttggt ttcgcaggcg aacttggcga aacggccgcg gattcccgct
     5521 gcgacttggc ggcgcgcttg ttgacgtaga gggcggcgtc ggcgcgtttg agcaagtcct
     5581 cgggtagctc tccacgaaca tagtccgtcc atcccgcggc gaaagaaaac tcctgcacct
     5641 ggcctgcgac ttcgatcttg agatccttca cacgaccgag gacgtggcgc acctcttcgg
     5701 gcttgcactc cgggagcaag accatgaact cgtcgccgcc gatgcgaacg gccaggtcgg
     5761 aaccgcgaat cgcgcgggcc aggcgggcgg cgagagtctt gatggcttcg tcacccgcgg
     5821 cgtggccgaa tttgtcgttg atgaatttca aaccgtttaa gtcgaacaga atgacggtca
     5881 gcgcgcgccc atggcgctgg gcgcgggaga tttcctcggc taggcgctgc tcgccggagc
     5941 ggcggttgta gaggccggtg agcgggtcga gagcggcgag tttgtagacc tcctcggtgc
     6001 gcacctctat gcgggtcatg gcgtcgaact gctcgctcat gtggcgctgc attcgattga
     6061 tttggaattg ctggtaaacg gcgttgatgt tgaacagcag caccagaccg accagccctc
     6121 gcacggcctg accgagctcg aaggcgtagg tggattcgga gtgcgcgaga agctcgggaa
     6181 aggcgaagga agccacggtc agcgccagca ggatagtgat cagaattgcg gaggaccaca
     6241 accaccactg gcgtcgtgcc acagaacgca gcttggcccg gaattcctcg ccctgcaagg
     6301 cgtgggcgga gggaggaggc gctggcttag gctgctcggc tgtatcggcc attggcgggt
     6361 aatcctaatt agatgcccaa acttctggca gggcaagaga gcggctggaa agtgatgagg
     6421 aaggagtaag aggcaggggt taggggagag cgaatcgtga atagagtaga gcaggagaac
     6481 agcacagcgg aaaagagatt cccagggccg cgggccgcgg cccgaggaat gactccgcga
     6541 cgaatctacg aagagagagg gacggccgga ggccggcgct acatcagggc aggcgcgtcg
     6601 cgcgcctcca gaatggcccg acagttcgcg gcgctacagg cccaggacgc gggccgcttt
     6661 gcgggtcgcg ccagcggaca ggaacagcac cgcgccgtaa tgtccagagc cggcggagac
     6721 cgcctgcaaa gcgccgacgt tgatgcgcgc cgcggcaagg cgggcgagga tgccggcgat
     6781 agcaccgggg cggtcgccgc cggaaatgta gaatgcggaa cttttcttga cgcgcagctt
     6841 cacgcgcttg gccgcggcgg cgaatgcggc gccgttcttg gggatgaatt cgagctgcgc
     6901 gcggccggcg cccgacggat agccccagaa ggcgattaag ttgatgccgg cgtcgcgcag
     6961 tgcgccgaga acgcgggcgc cttcgccggc cttgtgcggg atggatgcag aaaagtggtc
     7021 cacctggcgg atctggtctg gcatgacgac ctccttgcga agagagagcc gctattctag
     7081 ttcaaacaaa ggcggaaaag cagaaggcta agagtacatt aggagggggt gcatgcaatt
     7141 gactgcgtgg aaacgggaga gcatagtggg ggagtagttt gaaagacgac cttttcgggc
     7201 agaaggggtg tcgagatgta tcctacttca tcatatctgc caggcgatgc ccagcaccga
     7261 tttatgcgat ggataagatt cattcctgcg gccgaagcgg agacagcgcc gaggccgaag
     7321 gctgggagag tcgaaacatg aagtgcaatc ctgcagatag cagaagtagc aaccgctcat
     7381 ggctgagcgg aatggccggg cggaagtcga gcaggtcgct gaggcttgca ctgtgcggga
     7441 tggcttggat tgtgctgagc ggtctgatgg gtggctgctc cggcggcggg tcgatcacca
     7501 gcgggaacgg cggcgggccg gtacccgaag tggcgtttgc gattgacggc aatgcgaata
     7561 cggtttccgt gttcacagtg gacacgacga tcggaacgtg gacgccgatc ggaacaatcg
     7621 cgacagggac gaaaccgctg gcggtggcac tgcatccttc cggaaaattt ctctacgtgg
     7681 cgaattttaa ttccgcgaac atctcggcat acaacatcga ccccacgacc ggggcgctga
     7741 cggcgatgat gggctcgcca tttgccgcag tcaacgctcc gcaagcgctg gcgattgatt
     7801 cgagcggaaa tttcctctac gtctccacgc tgacggcggc gatgccgccc gttcatgtcc
     7861 tatcaggcta cagcatcaac gcgacgaccg gcgcgctaac gctgatcgcg cagccaaacg
     7921 tgccagccga cacgacgccg gtggggctca cgtttgctcc gacaaccgag tttctgtacg
     7981 cagcggacat gaacaataac gtgctgggat tttcggctca tgccgcgacg ggggtgctgg
     8041 cgcctgtgcc ggggaatcca ttcgcggcgg gtacgatgcc gtttggaatt gtggcgcacc
     8101 ctacgggtaa gttcgtctac gtaacgaata ttataagcgg cgatgtttcc ggctacttgg
     8161 tggatgcgct gacgggacga ctgacaccgg tgcctatgtc gccgttcacg agcggaacgt
     8221 cgccggggcc gattccggct accattgcgg tggagcccac gggcaagttc ctgtacgtgg
     8281 tgaacgccgg tgaagcggac atatcggagt attccatcga tgcgacgagc ggagcgctgg
     8341 caccgctcac cacgtcgccg ttcgttttgc cggcgggtac gatccttttg aggatggcgg
     8401 tggatccgac cgggactttt ctgtacgtgg tgaatttctc ggggaacaac atctccatct
     8461 tcatgatcca gagcaacggt tcgctcaatc cgatggggac gacgctgatg ggcgccggag
     8521 ccacgagcgg gattgccttc cgggccgggc atccgtagaa gagtggatag tggttcgtgg
     8581 aaagtgaaaa gagagaagtt gtcagtcttc agttttcatt cagaagagat ggaaatgcga
     8641 gcgccggaga atcgggcgac gcccgctctg aattcaatcc aaaagtttct gctccagaaa
     8701 acccgtccta cttcaaaagc tgctcgatgg ccgcgatgac ttcgggggaa tccggagtga
     8761 cgtccggctc gaagcgctgg acgactttgc cgtttcggtc gacgaggaac ttggtgaaat
     8821 tccacttgat gtcgccggcc acggcgggat tggaatcctt cgaagtgaga aattgataga
     8881 gtggcgcctg gtcgccgcct ttgacggaaa ttttggcata gagcgggaac ttgacgctgt
     8941 acttggtgga gcaaaacgtt ttgatttctt tgttggtgcc gggctcctgt gcgccgaaat
     9001 tattggccgg gaagcccagc acgaccaggc cacggtcctt gtatttttcg tacgtggctt
     9061 cgagagcggt gtactgcggg gtgtagccgc agcggctggc cacgttcacc aggagaagca
     9121 ccttcccctt gaaattggcg aggggcatgg gcttgccgtc gatggacggc agagtgtagt
     9181 cgtacaggtt tgaagcggca aagagagatg cagccatcat aagggtcacc aacccggcca
     9241 gaagatagca gcgaatattc gaacccacaa atgctttcat gaccggacaa tcctccaaga
     9301 aatcgcgtta caacagcgtg tgaagaccca aagagcatag cgaacaggca agccggatgc
     9361 aaccgccagc agtgggatga gggagaaact tttcgaggcg gtaagtgacc aggaggaatc
     9421 ggcaaccctg ggcgcgcggc ggcagattca gggtagcttc gctaacaggt cggcagggga
     9481 agcgttgtcg ctgaaggtgc ggctgatatg ggcgaagagg tcgcggttgc cggggccgaa
     9541 gacaaagctg ccgccgagct ggaagggatt cttgccgagc ttcacctggc gaaagccagc
     9601 ggcgcgggcg tgcttgcggg cagcggcgtt gtcacggcgg agaagatgga gcacattggc
     9661 ggagccgagg ccggcggcgc ggtaggcttc gcggttggcg tcaatgagca gaggaaacgt
     9721 gatgccggtt tcctcacgga aaattcgagc gtagcgaaag tcgccaaggc cgatggccgc
     9781 aaggcgtgcg cccctcgcgc ggaaggcgga ttcgttttgg cccaactggg caacgtgctc
     9841 gcggcagaag attcaaccgt agtggcggag gaaaaccagg accgccgcag aatcggacca
     9901 gagtgtgccg agttgcgcag agcgctcatc gcaatcggga aggacaatcc ccgccaaaat
     9961 cgaactgaga gaacgatcca ttggcggcaa ttgtggacgg ggagcgaggg caggtcaagc
    10021 tggggaaacg gtgggcttgc acccgtctgg agaagggggg gcttcgctcc gccccgtgtg
    10081 gacacgggcg gcccctacag aaaaaactac gagatgctga tggtgctgtt gagagttagg
    10141 aacgggacag aaaaacaacg gcggtcctca ggtgtcagtc tgcggaagaa ccgggtccgg
    10201 cctagcagct tcgccgggac aaattccgag cggcgctgca gcgcgacaat catcgaatgt
    10261 cgagaggcgt gaagacgccg ttctttatca cggcaaggcg aatgggccgc ggcgggcctt
    10321 tgcgcggcaa cgtgacttcc acccagagtt cttcacccgc ggggcgccgg gagggatcct
    10381 gaatgtgttc gggaatgaag aacgccacgg gctcaaacag gctgatggtg gggaccgagt
    10441 cgcggaaaac ctcccatacg gcagggtcgc tggtgcgatc ggaatagacg gcgacaagct
    10501 gcccgttctg gacttcaagt ctggcattgg cgtaaacccg cgcgggcgcg cgtccctccg
    10561 tgttgcggga ctccgggacc gcgaatccga taccggacat ctgcaggcga aggctgacgt
    10621 agcgtccgcg gatgggaaga tcgggatcgt agggcgcagc gcggacccac acgcgggggc
    10681 gcgtggcgcg gtcgtagaga agcttggcgc cgaggctgca aacgagcgcg acgtggaggc
    10741 cggcaatcag gagtccgcgc agggtgagct tcatgcttgg cctcccaggc gagcgacgag
    10801 acgccggcgc gttttctcga ggagccagcc aacgaagagg aaaagcacgc cgagaccgat
    10861 gaggccttcc gagcggccga gcttgtccat gacgtcggag aaataaaaga ccagaaccgt
    10921 cagagcgaag ccggcgatgc caaggttaat gcgttccttg cgcgattcgc gcaggcccca
    10981 cgcaatcagt cccacagaac cgagtccaca gagaaaatag ggtcccagct cgtgccaggc
    11041 gaattgcaga atcggaagct ggccgccttc cgcgttggat cccgtggtgc cgagaagcca
    11101 aacccacagc gccgccagaa gattgagggc agccgcgcgc ccgcgaagaa aaacggcgag
    11161 aagaagcggt agtccgaagg cgagcgtcca gcccagcgct gcgaggccga gcggaacgac
    11221 aggttcgtgg cgccagcggt agaagtccga ggctgattca atggcaaacg ggacggatgg
    11281 aatcagagct atcgcgccga ccacaaccag ggcgcggcga agcgggtgcc ggcgttcagc
    11341 gattgaaccg gtgaggtagg tgatcgcgag aagaagaagg ccttccgcga ggatgcgctc
    11401 gccgcccacg aagttttccg tggcgacgat ccactcgccg gcgagccagg caggcacgag
    11461 aagggcgacg agaagtccct ggatccaatc gcgcagaatc caccaggcca gcgcagcgcc
    11521 aatggcccag agcatgatgc cgccgggcca atgctcttcc aggttgaaga tctgcccggc
    11581 gaggaaaatt cccgccccca aggtggcggt gccgacggcg tggagaacgc gggaaagcgc
    11641 ggtgaagcgt ccggccgcgg cggcacccgc cgcgtggaac agtgccacca gcaggagaac
    11701 cagcgcgaag cgcttggacg gagaaagctc gtcccaatga gcggcgacga acaggagcac
    11761 ccccgcagct aggaggaggc cgccgaaact cagggcgaga atgattggcc agcgaagttt
    11821 ttgcgaaccg ctgtgctggg cttcgaagtc tcgaaggcgg tccgcggtgg ccgcgtcgac
    11881 gacgcccgcg ctgacccagc gttggagttg cgcttcaaga ttggccggca caaagggcac
    11941 tccctgccga taagatagcg ctttcggcac gcgaagaggc aagaaaaagt gggacgtgta
    12001 taaaattgtt tgcacactgc gaaagagaga ccaccggggc ggttttgtgc cttgacaaga
    12061 ggggcgcgac aagatatttc gctaaagaga gtttttcggt tcttggtgct cgggcggctc
    12121 ccagccggcc gctggggtgt gcgcctgcct gagagtgtgt agttcgaccc gatgacgacc
    12181 tgcccacaac gctttgagga gacccgaaca atgtccagga aagtgaccgg cgcgttcgcc
    12241 gtaatgctgg ggtgtggagt ggcgctgctg gggttgttgc cgaaggcccc gggacgatat
    12301 gcgcgccgga cgacgctcga gcgatccgcg acgcggccgg gagtggtgga cgcggggctc
    12361 gaggcgcggg tgcgcgaggc gctgaggagc gcgccgcttg ctttcgaggc caacctgggg
    12421 cagaccgacg agcaagtgaa attcacggcg gggggcgcgg gctacacgct gtttctgacg
    12481 ggggaagaag cggtgatcgc gctgcaccgc cctttcgcgc gggagaagaa accggtttat
    12541 ttggaggagc gggcgcaagg ctggcacgcc ggcgagccga tgagcgcggt cctgcgactt
    12601 aagtttatcg gggcgagtgc caccacgcgg ctgacgggaa tcgatgagca gccggcgcgg
    12661 acgaactatt tcatcggcaa cgatgagagc cgctggcgca ggaatgtgcc caactaccgc
    12721 gaggtgaggt acgaaaacct ttacccgggt gtagatgcgg ttttttacgg agatcggaga
    12781 acgctggaat atgacttcgt ggtgaagccg ggggcggacc cgagagtgat cacgctggga
    12841 tttgaaggcg cgcgaaatct gaaactgagc cccgcgggtg aactcgcagc ggaaacgccg
    12901 ctgggcgaag tgcggctgct ccaaccgcgg gtgtatcagg aaaccatcag cggcgcgcga
    12961 gtgccggtcg ctggaaagta cgtgcaacgc ggcgagggac tgatcggatt cgagctagcc
    13021 gggtacgacg cggggcgcgc cgtggtgatt gacccaccgg tgagcgtgat gccgcggatg
    13081 ctggcctcgc cgccgccacc ccccggaccg ccgacgccca cgggatcggc gctgcagctc
    13141 ggaacctatc taggggggca gttcgacgag gccatcaatg cgatcgccgt gggctcgaac
    13201 ggccaagtct atgtggccgg gttttcggat tcgttcagcg gctttcccgc ggctggcacg
    13261 cctttccagg gcttcaacgc cggcgctttc gactgctcgc agtttcctca gcatccctgc
    13321 ggcgatgcct ttgtggccac actgaacacg accacgggcg cgctggtcgc gaccactttt
    13381 ctgggcggcg gtgcggacga cgtagcctgg gggctggcgc tggacagcgc aaacgacccg
    13441 tacgtggtgg gcgagagcga ttcgggaaat tttccgacga cggccgcgac ggcgtttcag
    13501 gccacgccgg gcaacgcgag ctgcgcgagc ggatcaagaa cgcggccgtg ccggcacgcg
    13561 ttcttctcca agttgacacc caatctttcc tcgctcacgt attcgacgtt cctggccggg
    13621 acggatgacg actcggcgtt cgccgtggcc gtggacccca gcgggaatgc gttcatcacc
    13681 ggcgttgccg ggtttggctt cacgacggtg acgccggcaa gcggcgccta cagcggcgcg
    13741 ggcgacgcgt ttgttgcacg ggtcgatcca acggcgagtg ggacagggtc cttgatttac
    13801 gccacctaca tcggcgggtc gggaaccgac gaaggcctgg cgatagccgt tgacgtgaac
    13861 ggaaacgcgt ccatcggcgg cgctacattt tcgacggtca atacctccgt ggcgtttcca
    13921 accacgataa acgctgtgca ggcgggctcg tcggacgcca gcttttgcgg accgggaggc
    13981 gggttcaact gcggcgacgg atttatcgcc caggtggatg cgaccggcac actgacgtac
    14041 gcttcatttt tcggaggcga ctccaccgac caggtcaacg ggatagccct ggacgcggcg
    14101 ggcaacattt tcgtgacggg cgccacgagc tcgcattcga tccccattcc tacaacgccc
    14161 ggagcgtttg atactgccgg aagcagcggg ttcgatgcgt ttgccgcgaa gatcaatccg
    14221 gcggccgccg ggctggcagg agtgacgtac tcgacgtttc tggcggggag caatgacgac
    14281 agcggcaacg ccatcgccat cgatccgagt gggaacgcat tcattgccgg gaccacgaat
    14341 tcgacggatt atccgacttt cctggcgctg caggctgcat cgcgggtggg cttcttcagc
    14401 cagaccagtt ttgtgacctc gctcaatgcc gcgggatcgg cgctgaattt ttccacatat
    14461 tacggcggga cgaatacgga gaccggacgc gcggtcgcga ttgatgcggc ggggctgatt
    14521 tacgccggcg gctcgaccaa ctcgaccgac ctctgtacgg caaaggcttt tcaaccgcag
    14581 aacaattcgc agtttggaac cgcagacgcg ttcctgacga ttatcaatcc gaccaacacc
    14641 gcggcagtct gcgtgatgat gaccaatggg acgatgccac cggggccggt ggatttcggc
    14701 caggtggatg tgggcacgac gagctttcct caaacagtgg atctgttcaa cgccggcagc
    14761 gcggccatga cgttt
//
LOCUS       DASYVO010000393         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_25844, whole genome shotgun sequence.
ACCESSION   DASYVO010000393 DASYVO010000000
VERSION     DASYVO010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25844"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..905)
                     /locus_tag="VGR03_13265"
     CDS             complement(150..905)
                     /locus_tag="VGR03_13265"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1995293.1"
                     /translation="MSAISAVVYREAKIRATNFTFIFWDLFFPLLYMLVFGVGINTAL
                     GAPTGLGNVNYNEFFLAGILAMASFAIASNTSWSFFLDRDNGIFYEMLTYPMSRSEYL
                     LGKVIFNVIIAVVQAAITLAVGRILLGIRVQWPRLPLLLAGMAIGTAGWFFFYAIFAL
                     RIRRNDLFNSITSVFYFVFMFASSMFYPLEPLPVWFRRAALLNPITWEIDWLRYTSIG
                     IGNFHSILLEGAAFLVFSLACFAYAVRCLQQQE"
     gene            complement(902..1858)
                     /locus_tag="VGR03_13270"
     CDS             complement(902..1858)
                     /locus_tag="VGR03_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1995294.1"
                     /translation="MSEFALEVENLVKVYSSRGKTPIRAVDGMSFRVPRGIIFGLLGP
                     NGAGKSTLLRMLSTLTRPTEGTAKILGYDVVKKPIEVRKRISSVIQATAVELFLSVKD
                     NLLTYARFQGLDKTTAAHRAAALIEEFQLGPEVDRKVQDLSGGFRRRVQVAKVFMVDT
                     PVLFLDEFSTGMDPILKRQVMNLLRAEVEKGRTIILTTQVLSEAEELCDDILIINKGK
                     QIARGDLNTLKQLSQGVYDITLAFDRLPDTIQAEIAALTPIRFTVEGNTIELTLKIEE
                     SRVMDVVSALSRNRHVLRVEVRGASLEDIFVELTTREEKESA"
     gene            2020..>2853
                     /locus_tag="VGR03_13275"
     CDS             2020..>2853
                     /locus_tag="VGR03_13275"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1995295.1"
                     /translation="MRNIAFTLLIALLPYPAVAQKSTLPAPAQVAQEVRDYRMDNEDR
                     IVRELCEFLANPNIASDTPNIQKNAAHLVEMLEARGIETHLLPINGRGPVVFGKLITP
                     EAKRTVIFYAHYDGQPVDAAAWTDGKPFEPVLRDAAIEAGGKRIPFPENSAQHRAVYD
                     DNWRIYARSSSDDKSPIVALLAAIDALRAKKITLGVNLKVIFEGEEEAGSTNLQRTLE
                     LHKNLLGADLLITADGPVHQSGRPLVFFGNRGDIGIGVTVYGPVRALHSGHYGNWAPN
                     PA"
BASE COUNT          652 a          757 c          897 g          547 t
ORIGIN      
        1 aacagaatag gtgatggtac tgtgcccaat cagttgctag cggtccctgc atccaggtgg
       61 ggcgggcaaa caagaggcca ggagggagcg ccccctccag cgattttccc acagggggat
      121 cagagccctt caagaaccgg ctcggagcgt tattcctgct gttgaaggca gcgcacggcg
      181 taggcaaaac aggctagaga gaaaaccagg aatgctgccc cttcgagcaa aatcgaatgg
      241 aaattgccaa ttccgataga ggtgtagcgc agccaatcga tttcccaggt gatggggttc
      301 aggagcgcgg cccggcggaa ccagacgggg agcggttcga gcggataaaa catcgagctg
      361 gcgaacatga agacaaagta gaagacgctg gtaatgctgt taaagaggtc gttgcgcctg
      421 atccgcaaag caaagatcgc atagaagaaa aaccagccgg cagtgccgat ggccatcccg
      481 gcgagaagca gaggtagtct cggccactgg acgcgaatgc ccagcaaaat gcggccaaca
      541 gcgagggtta tggcggcctg cacgacggcg atgatgacgt tgaagatgac tttcccgagc
      601 aggtattcgc tgcggctcat gggataggtg agcatttcgt agaagatgcc gttgtcgcgg
      661 tcgaggaaga aggaccagga cgtgttcgag gcgatggcga aactagccat cgcgagaatc
      721 cccgccagaa agaattcgtt gtagttcacg ttgccgagcc cggtgggagc gccgagggcg
      781 gtgttgattc ccacgccgaa gaccagcatg tagaggagag ggaagaacag atcccagaag
      841 atgaacgtga agttggtggc gcgaatcttc gcttcgcgat agacgacagc ggaaatggcg
      901 ctcatgcgga ttccttctct tctcgcgtgg tcagctcaac gaaaatatcc tcaaggcttg
      961 cgccgcggac ttcgacgcgg agaacatggc ggttgcggga gagcgcgctg acaacatcca
     1021 tgacccggct ttcttcgatt ttgagcgtga gctcaatggt gttcccctcg actgtgaagc
     1081 ggataggggt gagcgcagca atctcagctt gaatggtgtc cggcaagcga tcgaacgcca
     1141 gggtgatgtc gtagacgccc tgggaaagct gcttcagcgt gttgaggtcg ccgcgcgcga
     1201 tctgttttcc cttgttgatg atgaggatgt cgtcgcaaag ttcttccgct tcgctgagaa
     1261 cttgcgtcgt caggatgatg gttcttccct tctcgacctc ggcgcgcaga agattcatca
     1321 cctggcgctt caggatggga tccatcccgg tggaaaattc atccaggaag agcaccgggg
     1381 tatcgaccat gaagactttc gcgacttgca cgcggcggcg gaaaccgccg ctcaagtcct
     1441 gcacttttcg gtcgacttcc gggccgagct gaaattcttc gatcagggcg gcggcacggt
     1501 gcgccgcggt cgttttgtcc agcccctgga agcgcgcgta ggtgagcaaa ttatctttca
     1561 ccgacaagaa cagctcgacc gctgtcgcct gaatcaccga cgaaatccgc tttcgtactt
     1621 cgatgggctt tttcacgaca tcgtagccga gaatcttcgc ggtgccttcg gttggccgcg
     1681 tgagcgtgga gagcatgcgc agcagcgtgg acttgcccgc gccgttgggt ccaagcagac
     1741 cgaagatgat tccgcgaggc acgcgaaaac tcatgccgtc gactgcgcgg atcggcgttt
     1801 ttccgcgcga agagtacact tttacgaggt tttcgacttc cagcgcaaac tcactcatgt
     1861 acggctccaa aatgatgccg cggaacctgc acaccgcgga aaaaaccggc aaacgagcag
     1921 agaccatacg ggtcagatag aatcgctggc aggccgcgcc gcgccagccg tgttgctctg
     1981 cgacagcaaa gccgtcggga gcgttagagg agggaaactt tgcgaaacat agcgttcact
     2041 ctgttgattg cccttttacc gtatccggcg gtcgcacaga agtcaactct tccggcgcct
     2101 gcgcaggtcg cacaggaagt gcgcgattac cgtatggata acgaggaccg catcgtccgc
     2161 gagctgtgtg aatttcttgc caaccccaac attgccagtg acacgccgaa tattcagaaa
     2221 aacgcggcgc atctggtcga aatgttggag gcgcgaggga tcgaaacgca cctgctaccg
     2281 atcaacgggc gcgggccggt ggttttcggc aagctgatca cgccggaagc gaagcgcacg
     2341 gtgatctttt acgcgcacta cgatggacag ccggtagatg cggcggcgtg gacggatggg
     2401 aaaccattcg agccggtgct tcgcgatgcg gccatcgaag ccggcggaaa gcgcatcccc
     2461 ttcccggaaa acagcgcgca acatcgggcg gtctatgacg acaattggcg gatttacgcg
     2521 cggtcgtctt cggatgacaa atcgccgatc gtggcgctgt tggcagcaat cgacgcactt
     2581 cgagcgaaga agattacgct cggagtgaat ttgaaagtga tcttcgaggg cgaagaagaa
     2641 gcggggtcga caaatcttca acgaacgctc gaactgcaca agaatttgtt gggggcggac
     2701 ctgcttatca cggccgatgg ccctgtgcat caatcggggc ggccgttggt tttcttcgga
     2761 aaccggggcg acatcgggat cggcgtcacg gtttacggac cggtacgcgc gctgcatagc
     2821 gggcactacg gaaattgggc gccgaatccg gcg
//
LOCUS       DASYVO010000394        10944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_80342, whole genome shotgun sequence.
ACCESSION   DASYVO010000394 DASYVO010000000
VERSION     DASYVO010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 10944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10944
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80342"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VGR03_13280"
     CDS             complement(<1..895)
                     /locus_tag="VGR03_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995296.1"
                     /translation="MKDKTGPKSRNSRPARPRFSGESAINPTRREFLRTAAGASLALA
                     GSPIAAWADHHGHPSPNSISYLDRRMYIHNMELLAHFMPGHVRNGKMQIMSIGNRRYM
                     FQQGDVIDVSDVRKPIFYNKGGFEGGQVQVAYNKNLKKWILMTGAQTPITDSTPEAPM
                     GKYDDPHLDDKRKNYKGLRGVRFYDVTDPSKIVKLSEFSTGATGQGTHRNFYDGGKYA
                     YLDTAPDETFIHQPGYFRQLVNGNMIVNASDPSSAKEVSMWWVPGSRKGEETEYNKWI
                     WSKLVPPKVVADQTPFAGLHGP"
     gene            1299..2672
                     /locus_tag="VGR03_13285"
     CDS             1299..2672
                     /locus_tag="VGR03_13285"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEV1995297.1"
                     /translation="MAIATTNPATGQVLKTFEPLSDAQIEVKLQKAADTFSSYRKVPF
                     AERAQMMLKAAAILDGEKEIFARVMTTEMGKTLRSAVDEAAKCAVVCRYYAENAERFL
                     ADEVVETTASRSYIRYQPLGAVLAVMPWNFPFWQVLRFAAPALMAGNVGLLKHASIVP
                     QSALAIEDIFRRAGFPDGAFQTLLVGSQKVDKILGDPRVMAATLTGSEGAGIEVGIGA
                     AKRIKKVVLELGGSDPFIVMPSANLEVATTTAVKARIFNNGQSCIAAKRFIVAESIAD
                     KFERLFAEKMGALKVGDPFDEKTDVGPMSTADGLADLDRDVKKTVEAGAKVLTGGKRL
                     DRPGNFYAPTVLTNIPKGSPAHKEELFGPVASVFRAADLDDAIRIANDTRFGLGASAW
                     TNDKNERERFINDLESGMVFINRMVASDPRIPFGGVKWSGHGRELGAHGIREFTNIKT
                     VWIEEAP"
     gene            2831..3532
                     /locus_tag="VGR03_13290"
     CDS             2831..3532
                     /locus_tag="VGR03_13290"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEV1995298.1"
                     /translation="MYKIVRSSRLYEQIVQQVEESIHKSALKPGDQLPPERELAQQFG
                     VSRTAVREAVKALREKGLVEAYPGRGTFITDGTSYSMRQSLDRMLKIGQPEGSAYLAE
                     VREILEPEIAALAATRVDEEALASMREQIAVMDGARKDPDAFIEADLDFHLTLAEAAA
                     NPLILSLIDSIVGLLREQRMGIFQVEGGPERGQYHHKRILEAVEHRDPLGARDAMKAH
                     LRQVREDSRRAPGGS"
     gene            3752..4633
                     /locus_tag="VGR03_13295"
     CDS             3752..4633
                     /locus_tag="VGR03_13295"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1995299.1"
                     /translation="MANLGFVGLGVMGSEMVNRLLGKGHSVTGYNRTRSKAEWLVKKG
                     MKWADSPRAVVAGSDVAFSMVTNSVALGAVMNGPDGMLAAVTPGKIFVDMSTVSPSYS
                     REIAAQVREKGGDMVDSPVSGSVITLQEGKLSVMVGGRKETFEKVKPLLYDIGPKVTH
                     VGDNGLALVMKIGTNLSLAVQMLAFCEGVLLAEKSGIARETAVDVLTHSVIASPMVQY
                     RGPFILKMPEEAWFNVNMMQKDMNLALELGRQLDVPMPTTAITNEILTAARGMGLVEK
                     DFAVVFDVLAQMSGVKP"
     gene            4630..5634
                     /locus_tag="VGR03_13300"
     CDS             4630..5634
                     /locus_tag="VGR03_13300"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV1995300.1"
                     /translation="MTLATDQSKSKANPTGKSEQWMRMYRRMVMIREFEEQVNELYTR
                     ALMPGLAHLYIGEEAVAVGICEALSGDDYITSTHRGHGHCVAKGASPDRMFAELLGKE
                     AGYCRGKGGSMHIADPATGNLGANAIVGGSTGIATGAALSAKRLGTGQVAVCFFGEGA
                     LGQGVLYEVMNLAALWKLPVIYVCENNLYTEYTHYSETTAGDILARGTAFGVQAESVD
                     GQDVRAVYATAHRLVERARKGEGPAFLVCNTYRYTGHHVGDINREYYRSKQEELEWKT
                     KRDPIKNFSQWLVGQKFSDEEKMKQIQEEVRAEMKTAVEFAIAAPYPTVDQVDQDVYA
                     "
     gene            5627..6619
                     /locus_tag="VGR03_13305"
     CDS             5627..6619
                     /locus_tag="VGR03_13305"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV1995301.1"
                     /translation="MPEAATRELTFAQAIREALAEEMRRDKRVCIFGEDVAEAGTPFK
                     VLSGLVEEFGAERVMDTPISEAGFTGMGVGAAMTGLRPVVDIMFGDFLTLTMDQMVNQ
                     AAKVHYMSGGTWKVPMVLRTTLGATRRSAAQHSQSLHAWLSHVPGLKVVMPSTPYDAK
                     GLLKTAIRDENPVVFFEDKMMYKVKGPVPEEEYTIPFGAADVKREGEDITIVATSSMV
                     QVALGAAELLEKIGVSAEVIDPRTTWPLDEKTLVESAKKTSRAMVVDEGYGRYGVTGE
                     IASVIAEGAFYSLEAPVKRMGAMHVPIPFSPPLEDVTVPTEQTVFEAARALCGK"
     gene            6647..7993
                     /locus_tag="VGR03_13310"
     CDS             6647..7993
                     /locus_tag="VGR03_13310"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEV1995302.1"
                     /translation="MALKTYGTMAVDWENRIDFDRLRRERLARAKALLAKSEMGALLC
                     FDMNNVRYLTATHIGTWAQDKISRFSLLPQNDEPILWDFGSAARHHQQNCPWLGERSR
                     AGIPLLRGAMSPEMGRAENVARKIKVELEKRGLHKEPLGIDVVEPPILFALQKEGITI
                     VDGQQLMSDARVIKTQDEISLLNHSAMMVDAAYDELYRAMKPGMSENQAVGLASKVLY
                     DLGSEYVEAVNAISGERCNPHPHVFSDRVLRPGDPVYYDILHSYMGYRTCYYRCFTIG
                     YASHAMIDAYKRCREYLDAAISLVQPGRTTAEIAAVWPKAEEFGFPDEEAAFALQFGH
                     GIGLAIWEKPVISRLVSLEHSHEIKPGMVFALETFWPSTDGWSAARIEEEIVVTETGH
                     EVITRFPADQLMVAGAHYHTVNGPLATTRENEAPPSKRVKEMVAASAKAEGVAATD"
     gene            8024..9220
                     /locus_tag="VGR03_13315"
     CDS             8024..9220
                     /locus_tag="VGR03_13315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEV1995303.1"
                     /translation="MAISVVMPALEMAQETGKLLAWRKKEGERVTKGEPLLEIETDKA
                     VVEVEAPGDGILAGITAEVGAVIPVGQTIAWLVAQGEKPPANTATAAPAARASSGLER
                     KVVAATVPVAAQHVAASPQISPKARRLAREIGVEISQICGSGPDGTITSEDVQRAAVL
                     KGASVGAAAAPAGVEALSQVARLMAERTTQSWTSVPHFFLVRDLDAGELIAAQKRIGQ
                     EMEKAQGAGATITDLLIALVARTLAKHPRMNSSWTGEGIRSNPEINVSVAMAVKDGVV
                     GAVIHKANTAKIAEISTLRRELTERARAGRLRPADITGGTFTLSNLGMYKVDAFSAII
                     TPPQAAILAVGSISDHVVPVGGKPCIRPMMTMTLSSDHRVVDGARAAEFLSELADAIR
                     EPEKVL"
     gene            9292..10557
                     /locus_tag="VGR03_13320"
     CDS             9292..10557
                     /locus_tag="VGR03_13320"
                     /inference="COORDINATES: protein motif:HMM:NF002461.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEV1995304.1"
                     /translation="MTPPNPRKKAFYANSSVQVLFAIGAALALGYFRPAAAIAMKPLG
                     DAFIRLITMIITLIVFCTVVTGIAGMEDMKKVGRVGGKALIYFEVVSTLALFIGLVVG
                     NVLHPGSGFNINAATLDAKAVADYAGQARAQSISDFLMHIIPNTVVDAFAKGDILQVL
                     LVSVLFGFALSMAGPRCKPLLDFLNLLTEAVFGIVKILMRFAPIGAFGALAFTIGKYG
                     VSSLGPLAKLIGTFYITSILFVFIVLGVIARLAGFGILRFLSYIKEEILLVLAISSSE
                     PAMPTLMAKLEKLGCSKALVGLVVPTGYTFNTDGTSIYMTLSALFVAQATNTPLTLMQ
                     QLTILAVAVLTSKGASGVQGASFIALVATMTVIPTIPVAGMALILGIDRFMSMCRATV
                     NMIGNGVATVVVARWENEIDRETLKHNLA"
     gene            10643..>10944
                     /locus_tag="VGR03_13325"
     CDS             10643..>10944
                     /locus_tag="VGR03_13325"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEV1995305.1"
                     /translation="MAERFDFDIVVAGAGHNSLIAAAYLAKAGYRCVVLEGRPIVGGG
                     VKSAQLTLRGFTHDICSSAHNGIQGNPILRDDELKLGEYGLEYIYPDPVYHMPFPDG"
BASE COUNT         2417 a         2607 c         3719 g         2201 t
ORIGIN      
        1 ccgggccgtg cagcccggcg aacggcgtct gatcggcgac gaccttgggg ggaaccagtt
       61 ttgaccagat ccacttgttg tattcggttt cttcgccttt gcgcgatccg ggaacccacc
      121 acatggaaac ttctttggcg ctggaaggat cggaagcatt cacgatcatg ttgccgttga
      181 ccaactgtct gaaatagcca ggttggtgga tgaaagtttc gtccggggcg gtatcgagat
      241 aggcgtactt tccaccatcg taaaaatttc ggtgcgtgcc ctggccggtg gcgccagtgc
      301 tgaactcaga aagtttgaca attttcgatg gatcggtgac atcgtagaaa cgcacaccac
      361 gaagtccctt gtaattcttg cgcttgtcat cgagatgcgg gtcgtcgtat ttgcccatgg
      421 gcgcttcggg agtcgaatcg gtaatcggcg tttgcgcgcc ggtcatgaga atccactttt
      481 ttagattctt gttgtaggcc acctggacct ggccgccttc gaatccgcct ttgttgtaaa
      541 agatgggctt tcgaacgtcg ctgacgtcga tcacgtcgcc ctgctggaac atgtagcggc
      601 ggttgccgat ggacattatt tgcatcttgc cgttacgcac gtggccgggc atgaaatggg
      661 ccaggagctc catgttgtgg atgtacatcc tgcgatcgag atacgagatg ctgttcgggg
      721 acgggtggcc gtgatggtcc gcccaagctg caatgggaga accagccaac gccaggctag
      781 cgcccgcggc ggttcgcaag aactcgcgcc gcgtcggatt gatggcgctt tcccctgaga
      841 atcggggcct tgcgggcctg gaattgcgtg atttcggccc agttttgtcc ttcatgagat
      901 gcggctcctt gtgttacaaa ctcgtggctt gcaccttttg cgattacaga cgatgacctt
      961 ggcctccccg ttcgaatccc cgtacctttt caccccttaa cgcgctcaat atacaagagg
     1021 ggtaggaaga gcatctcccg gtattcaaaa ggtaagaccg ctgtaccact cgcaaacaga
     1081 ggctgtcaag aatattcttc tatggattag agatgcttgc agttagtatg atttgcgaaa
     1141 aagcgttcgg gtatagtggt tggaccaaat cgagagggtg ttgacgccgg ctgcgaatgg
     1201 ttccgcagtc gttcgaagga caggcaggag aaagcggcga tacgatggga atcggtaacg
     1261 atcgagggtt acagtagcag ttcagaggga gaacagaaat ggcaattgca actaccaatc
     1321 cggcgacggg gcaagtcctt aagacgttcg aaccgctatc ggatgcccag attgaagtga
     1381 aactacagaa ggctgcggat acgttctcga gctaccgaaa agtgccgttc gccgagcgtg
     1441 cccagatgat gctgaaggcg gcggcgattc tggatggcga aaaagagatt tttgcgcgcg
     1501 tgatgacgac cgaaatgggc aagacgttgc ggtcggcggt tgatgaagcc gcgaagtgcg
     1561 cggtggtctg ccgctactat gccgaaaacg cggaacgctt tctcgccgac gaagtggtgg
     1621 agacgacggc gagccggagt tacatccgtt accagccgct cggggcggtg ctagcggtga
     1681 tgccgtggaa ttttccgttt tggcaagtgc tgcgatttgc cgcgccggcg ttgatggccg
     1741 ggaacgtcgg acttctgaag cacgcatcga tcgttccgca aagtgcgctg gcgatcgagg
     1801 acatcttccg gagagcgggg tttcccgacg gcgccttcca gacacttctg gtgggctcgc
     1861 aaaaagtgga caaaatactg ggcgatccgc gggtaatggc ggccacgttg acgggaagcg
     1921 aaggagcggg aattgaggta ggaatcggcg cggcgaaaag aatcaagaaa gtggtgctgg
     1981 aactgggagg aagcgacccg ttcattgtga tgccgagcgc gaatctggaa gtggcgacga
     2041 caacggcggt gaaggcccgg atcttcaaca acggacaatc gtgtatcgcc gccaagcgct
     2101 tcattgtggc cgaatccatc gcggacaaat ttgagcgcct attcgcggaa aaaatggggg
     2161 cgctgaaggt cggtgacccg tttgatgaga agacggatgt ggggcccatg tcaacggcag
     2221 atggcctggc ggatctcgac cgcgacgtga agaagacggt cgaagcaggg gcgaaagtct
     2281 tgaccggggg caaacggctg gatcgcccgg ggaattttta tgctcccacg gtactgacaa
     2341 atattccgaa gggctcgcca gcacacaagg aggaactgtt cggtcccgtg gcgagcgtgt
     2401 tccgcgccgc agacctggat gacgcgattc gaatcgcgaa cgacacacgc tttggattgg
     2461 gggcgagcgc ctggacgaat gataagaatg agcgcgaacg cttcattaac gatctggaat
     2521 ctggaatggt tttcataaac cgtatggtgg cttccgatcc gcgtataccg ttcgggggag
     2581 tgaagtggtc cggtcatggg cgagaactgg gagcccatgg aattcgggag ttcacgaaca
     2641 tcaagacggt atggatcgaa gaggcaccct agggagttgc gaaagcaaag gtgcgtgtgg
     2701 tagattggtc caacaactca accagaatct gacgagtccc acgtcaaacg gctgtactta
     2761 agcctctttt acttggtaca gcggtcgtac cagggtgcgg aatagtctct ttttggcttg
     2821 ggaggatatc atgtacaaaa tcgtgcgctc ctctcgcctg tatgagcaaa tcgttcagca
     2881 ggtcgaggaa tccattcaca aaagtgctct gaagccggga gaccaacttc cccccgaacg
     2941 ggagctagct caacaatttg gcgtgagccg gaccgccgtc cgcgaagcgg tgaaagcgct
     3001 gcgagaaaag ggactggtgg aagcctatcc gggccgcgga acctttatca ccgacggcac
     3061 ctcgtattcc atgcgccagt cgctggatcg gatgctgaaa attggtcagc ccgaggggtc
     3121 tgcctatctt gcggaagtac gcgaaatcct agagccagag attgctgcgc tagcggcgac
     3181 tcgcgttgac gaagaagctc tggcgtcaat gcgggaacaa atcgcagtaa tggatggtgc
     3241 ccgcaaggac cccgatgcgt ttatcgaagc ggatctcgat tttcacctaa ctcttgcgga
     3301 agccgcagca aatcccctga ttctttcgtt gatcgattcg attgtaggat tgctgcgtga
     3361 acagcggatg ggaatctttc aggtggaagg cggcccggag cgcgggcagt atcatcacaa
     3421 gaggatactg gaggccgtcg agcaccgcga tccactggga gcgcgagacg caatgaaggc
     3481 ccacttgcgg caggtgcgag aggattcgcg gcgggcgccg ggaggatcgt aagaagggtt
     3541 ttcagttgtc agttataagt tttaagtaag agaagaaaaa gaattggatg gatttttgtt
     3601 tttttggttt gagcgatgga agagtcataa gtcttaagtt tttagtaaga aaaggaaaag
     3661 aattaaatgg agttggattg tttttttgga tggtgtagcg gaagagtttt cagatgtcag
     3721 ttttgagttt ttagccggag aaaaaggatt catggcgaat ctgggatttg tcggacttgg
     3781 agtgatgggc agcgaaatgg tgaaccggct gctcgggaag ggccacagcg ttacgggcta
     3841 caaccgtacg cgttcgaaag cggagtggct cgtcaagaaa gggatgaagt gggcggactc
     3901 gccacgcgcg gtggtggcag ggtcggacgt tgcgttttcg atggtgacga attccgtggc
     3961 gcttggcgcg gtgatgaatg gtccggacgg aatgctggct gccgtgacgc ccggcaagat
     4021 tttcgtggat atgagcaccg tgagcccttc ctacagccgg gagattgcgg cccaggtgcg
     4081 ggaaaagggc ggcgacatgg tggactcgcc ggtatccggg agcgtgatta cgctgcaaga
     4141 gggaaaattg tcggtgatgg tgggaggccg caaggaaacg tttgaaaaag tgaagccgct
     4201 gctctacgac atcgggccaa aagtcaccca cgtaggcgat aacggcctgg cgctggtgat
     4261 gaagatcgga acgaacctga gccttgcggt gcagatgctg gcgttttgcg aaggcgtctt
     4321 gctggcagaa aagagcggga tcgcgcggga aactgcggtg gacgtgctga cgcacagcgt
     4381 gattgcatcg ccgatggtgc agtaccgagg gccgttcatt ctgaagatgc cggaagaagc
     4441 gtggttcaac gtcaacatga tgcaaaagga catgaatctg gcgctggagc tgggacggca
     4501 actggacgtg ccgatgccga cgacggcgat cacgaacgaa attctgacag cggcgcgggg
     4561 aatgggattg gtggagaagg attttgccgt agtgttcgat gttctggcgc agatgtccgg
     4621 agtgaagcca tgacgcttgc aacggatcaa tccaagtcca aagcaaaccc caccgggaaa
     4681 tccgagcagt ggatgcggat gtaccggcgg atggtgatga tccgggagtt cgaagaacag
     4741 gtcaatgaac tttacacgcg ggcgctgatg cccgggctgg cgcaccttta cattggcgaa
     4801 gaagcggtgg cggtgggaat ttgcgaagcg ctgagcgggg acgactacat aacgagcacg
     4861 caccgtgggc atgggcattg cgtggcgaaa ggggcttcgc cggaccggat gttcgcggag
     4921 ttgctgggaa aagaggcggg atattgccgg gggaaaggcg ggtcgatgca cattgccgac
     4981 ccagcgacgg gcaatttggg cgcgaatgcg atcgttggcg ggagcacagg cattgcaacg
     5041 ggtgcggcgc tgtctgcaaa gagattggga acgggacaag tggcggtgtg tttttttggc
     5101 gaaggagcgc tggggcaggg agttttgtat gaagtgatga acctggcggc attgtggaaa
     5161 ttgccggtga tttacgtttg cgaaaacaat ctttatacgg aatacacaca ctattcggag
     5221 acgacggcgg gcgacattct ggcgcgggga actgcttttg gagtgcaagc ggaaagcgtg
     5281 gacgggcagg acgtgcgcgc ggtgtacgca accgcgcacc ggctggtgga gcgggcgcgg
     5341 aagggcgaag ggccggcgtt tctggtgtgc aacacgtatc gctacacggg gcatcacgtg
     5401 ggcgacatca accgggaata ttaccgttcg aaacaagaag agcttgaatg gaagacgaag
     5461 cgggacccga tcaagaactt ttcgcaatgg ctcgtcggac aaaagttttc ggatgaggaa
     5521 aagatgaagc aaatccagga agaagtgcga gcggagatga aaacggcggt ggaatttgcg
     5581 attgccgcgc cgtatccgac ggtagaccag gtggaccagg acgtgtatgc ctgaagcggc
     5641 tacgagagag ctgacgtttg cacaagcgat ccgcgaagcg ctggcggaag agatgcgccg
     5701 cgacaagcgg gtgtgcattt tcggcgagga cgtggcggag gcgggaacgc cgttcaaagt
     5761 gttgtccggg ctggtggagg aattcggcgc ggaacgggtg atggacacgc cgatttcgga
     5821 agcaggtttt acaggaatgg gagtgggggc cgcgatgacg ggtttgcggc cggtggtgga
     5881 catcatgttc ggagattttt tgaccctgac gatggaccag atggtgaatc aggcggcgaa
     5941 ggtgcactac atgtccggcg ggacgtggaa agtgccgatg gtgttgcgga cgacgctggg
     6001 agcgacgcgc cgctcggcgg cgcagcattc gcagtcactg cacgcgtggc tgagccatgt
     6061 gccgggattg aaagtggtga tgccctcgac gccgtacgat gcgaagggac ttttgaagac
     6121 ggcgattcgc gacgagaacc cggtggtctt ctttgaagac aagatgatgt acaaggtgaa
     6181 agggccggta ccggaagagg aatatacgat tccattcggt gcggcggacg tgaagcgtga
     6241 gggcgaagac atcacgattg ttgcgacgag cagcatggtg caagtggcgc tgggggcggc
     6301 ggagttgctg gaaaagattg gtgtgagcgc ggaagtgatc gatccgcgga cgacgtggcc
     6361 gctggacgag aagacactgg tggagtcggc gaagaagaca tcgcgagcca tggtggttga
     6421 cgaaggttat ggacgctacg gggtgacggg agagattgct tcagtgattg cggaaggcgc
     6481 gttctatagc ctggaggcgc cggtaaagcg gatgggggcg atgcacgtgc cgattccatt
     6541 ttcgccgccc ctggaagatg tgacggtgcc gacggagcaa acggtatttg aggcggcgcg
     6601 ggcgctttgc gggaagtgat gagttgcgcg gaatgagagg tgtgagatgg cgctgaaaac
     6661 ttacggaaca atggcggtgg attgggaaaa ccgcattgat tttgaccgct tgcgccgcga
     6721 acggctggcg cgggcgaaag cgctgctggc gaaatcggag atgggggcgc tgctgtgctt
     6781 cgacatgaac aacgtgcggt atctgacggc gacgcacatc gggacatggg cgcaggacaa
     6841 aatcagtcgg ttttcgctgc tgccgcaaaa tgacgagccg atcctttggg atttcggatc
     6901 ggcggcgaga catcatcaac aaaattgccc gtggctcggc gagcgttcgc gcgcgggaat
     6961 tccgcttctg cgcggagcga tgtcgccgga gatggggcgc gcggaaaacg tggcgcggaa
     7021 aataaaggtg gaactggaaa agcgcgggct gcacaaggaa ccgctgggga tcgacgtggt
     7081 cgagccgccg attcttttcg cgctgcaaaa agagggaatc acgattgttg acgggcagca
     7141 gttgatgagc gatgcgcggg tgatcaagac gcaggacgag atttcgctgc tgaatcactc
     7201 ggcgatgatg gtggatgcgg cgtacgacga actgtaccgg gcgatgaaac cgggcatgag
     7261 cgagaaccag gccgtggggc tggcgagcaa agttttatac gacctgggct cggaatatgt
     7321 ggaagcggtg aatgcgattt ccggcgagcg gtgcaatccg catccgcacg ttttttcgga
     7381 tcgcgtgctg cggccgggag atcccgttta ttacgacatt ttgcattcct acatgggcta
     7441 ccggacttgc tactaccggt gcttcacgat cgggtacgcg tcgcacgcga tgatcgatgc
     7501 gtacaaacgc tgccgcgagt atctggacgc ggcgatttcg ctagtacagc cggggcgcac
     7561 gaccgcggag atcgcagcgg tgtggccgaa ggcggaggag ttcggatttc cagatgaaga
     7621 ggcggcattt gcgctgcaat tcgggcacgg gatcgggctg gcgatttggg aaaagccggt
     7681 gatcagccgg ctggtgtcgc tcgaacattc gcacgagatc aagcccggga tggtgttcgc
     7741 gctggaaacg ttttggcctt caacggatgg gtggagcgcg gcgcggattg aagaagaaat
     7801 tgtagttacg gaaaccgggc atgaagtcat tacgcgattt ccggcagatc agctgatggt
     7861 ggcgggcgcg cactatcaca ccgtcaatgg accgctggca acaacgcgcg agaatgaggc
     7921 gccgccgagc aaacgggtga aagaaatggt ggcagccagc gcgaaggccg aaggagtggc
     7981 agctacggac tgaaggcttg tccccggctc ctgacaaaaa actatggcga tcagtgtggt
     8041 aatgcccgcg ctggagatgg cgcaagaaac cgggaagctt ctggcctggc gaaaaaaaga
     8101 aggcgagcgc gtcaccaagg gcgagccgct gctcgaaatc gagacggata aggcggtcgt
     8161 cgaggtggaa gcgccggggg acgggattct tgcagggata acggccgaag tcggagcggt
     8221 gatccccgta ggacaaacga ttgcatggct cgtcgcgcag ggcgagaaac ctcccgcaaa
     8281 tacggcgacg gctgcacctg ccgcgcgtgc atcgagcggc ctggagcgaa aagttgttgc
     8341 cgcaactgta ccggtcgcgg cacaacacgt agcggcgtct ccgcagattt ccccgaaggc
     8401 gcggcggctg gctagagaaa ttggcgtcga gatcagccaa atttgcggat cgggtcctga
     8461 tgggacgatc acgtcagaag atgtgcagcg cgcggctgtt ttgaagggcg cgtctgttgg
     8521 ggctgcggcg gctccggccg gagtggaagc gctgagccag gtcgcgcggt tgatggccga
     8581 acgcaccacg cagagctgga ccagtgtgcc gcactttttt ctggtgcggg acctggacgc
     8641 gggggaattg attgccgcgc agaaacgaat cggacaagag atggagaaag cgcagggcgc
     8701 gggcgcgacc atcacggatt tgctgattgc acttgtggcg cgaaccttgg ccaagcatcc
     8761 gcgaatgaat tccagttgga cgggcgaggg aattcgttcg aatccggaga tcaacgtcag
     8821 cgtggcgatg gcggtgaagg atggcgtagt cggcgcggtg attcacaagg cgaacactgc
     8881 gaagatcgct gaaatctcca cgctgcggcg agaattgacg gagcgcgcac gagcgggccg
     8941 attgcgtccg gcggacatta ccggcgggac ttttacgctg agcaaccttg gcatgtacaa
     9001 ggtcgatgct ttcagcgcga tcatcacgcc gccgcaagcg gcgattctag cggtagggag
     9061 catttccgat catgtcgttc ccgttggggg aaaaccctgc atccggccga tgatgacgat
     9121 gacgctttcg agcgatcacc gcgtggtgga cggagcccga gcggcagagt tcttgagcga
     9181 attggcggac gccattcgcg agcccgagaa ggtgttgtag tggttttgtt cacaaaaatc
     9241 cttggaaggg aaaagaatac ttctcgcgcc gcgtagctcc cggcccctat tatgacgccg
     9301 cccaatccgc gcaagaaggc tttctatgca aattcgtcgg tgcaagttct gtttgcgatt
     9361 ggcgcggcgc ttgcgctcgg ctactttcgg ccggcggcgg cgatcgccat gaaaccgctg
     9421 ggcgatgcat tcatccggct aatcacgatg atcattacgt tgatcgtctt ttgcaccgtg
     9481 gtgaccggta tcgcaggcat ggaggacatg aagaaggtcg gacgggtcgg cgggaaagca
     9541 ttgatctact tcgaggtggt gtcaacattg gcgctgttca tcggcctggt ggttggcaac
     9601 gtgctacacc cgggcagcgg attcaacatc aatgcggcca cgctggacgc caaggcggtg
     9661 gcggactacg cggggcaggc aagagcgcaa agcatttcag atttcctgat gcatatcatc
     9721 cccaatacgg tggtggacgc ttttgccaag ggcgatatcc tgcaggtgct gctggtctcg
     9781 gtgctgtttg gatttgcgct gtcgatggcg gggccgcggt gcaagccact tttggatttt
     9841 ttgaatttgt tgacggaggc ggtgtttggg atcgtcaaga tcctaatgcg ctttgcgccg
     9901 atcggcgctt ttggcgccct ggcattcaca atcgggaaat acggcgtctc ctcgctgggg
     9961 ccgctggcca agctgatcgg cacgttctac atcacatcca ttttattcgt gttcattgtg
    10021 ctgggcgtga tcgcgcgcct ggcgggcttc gggatactga gattcctttc ctacatcaag
    10081 gaagagatcc tgctcgttct ggccatcagc tcgtcggaac cggccatgcc gacgctgatg
    10141 gccaaactgg aaaagctcgg atgttccaag gcgcttgtgg ggctggtggt gccgaccggg
    10201 tatacgttca acaccgatgg gacaagcatc tacatgacgc tgtcggcgct gttcgtggca
    10261 caagccacga atacgccgct gacgctgatg cagcaactga caattcttgc ggtggcggtg
    10321 ctgacttcca agggagcgag cggcgtgcaa ggcgcgtcat tcatcgcgct ggtggcgacg
    10381 atgaccgtga ttcccacgat tcccgtggcg ggaatggcgc tgattctggg gattgaccgg
    10441 ttcatgagca tgtgccgggc gaccgtgaac atgatcggaa atggagtggc caccgtcgtg
    10501 gtagcgcgct gggaaaacga aatcgatcgc gagacgctga agcacaatct agcgtgaagg
    10561 aagacttggg cccagggcac ttgcgccttg atccgcagga gcaggaagcc cagagaattg
    10621 agaatcgaag cggcaggctg ccatggcaga acgattcgat ttcgatatcg ttgtcgcagg
    10681 cgccggacac aatagtctga ttgcggctgc gtatctggcg aaagcgggct atcgctgcgt
    10741 ggtgctggag ggccggccaa tcgtgggcgg cggcgtcaaa tccgcgcaat tgacgttgcg
    10801 cggattcacg catgacattt gttcttcggc gcacaacgga atccaaggga atccgatcct
    10861 ccgcgacgat gagctgaaac tcggagagta cggcctcgag tacatctatc ccgatcctgt
    10921 ctatcacatg ccgtttcccg acgg
//
LOCUS       DASYVO010000395         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_94912, whole genome shotgun sequence.
ACCESSION   DASYVO010000395 DASYVO010000000
VERSION     DASYVO010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94912"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2498)
                     /locus_tag="VGR03_13330"
     CDS             complement(<1..2498)
                     /locus_tag="VGR03_13330"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV1995306.1"
                     /translation="MKLLGRKLRVSLSLVMALLAMMAVSASGQDIKIGYEKFVLPNGL
                     TVLVHEDHKAPIVALNIWYHVGSKNEKPGKTGFAHLFEHLMFGGSENLKGRYIDAMER
                     VGATDLNGTTNSDRTNYFEDVPTSALDYALFVESDRMGHFYNTINKETLDLQRGVVQN
                     EKRQGDNQPYSISRYLITENTYPAGHGYSWTTIGSMEDLNAASLADVQEWFKTYYGPS
                     NATLVIAGDIDVKTAREKVQKYFGDIPAGPPIAHQNEWVAKMTGVHRQQAQDRVPVAR
                     VFNIWNVPEYGQADTDYLDLVSDVLAAGKDSRFYKRLVYDEQVATSVNSGINPNEIGG
                     QFSIGAAAKPGGSLAPINKDVQEELDRFLKDGPTAEELERVKTAYEANLVRGLDRIGG
                     FGGKSDILARGQVFTGNPEQYLISLDRVRKATAQDLRDAARRWLSDGLYQLEVVPYPE
                     YKAASAGADRSKLPEVGATPNVKLPAFERMTLSNGLKVVLAERHELPLVNFTMLVDAG
                     TSADSPATPGTAAMSSSLLTSGTDKYNALQISDEIQRLGAELEAGSGLDTSTVYLSSL
                     KSKLEPSLALFSDVLLHPSFPQEDFARQQKLQIAAIQRAKTSPGAMAGRVMTPALYGK
                     DHPYGLELTEAGVAKLTRDAIAKFHDTWYRPKNTTLVIVGDTTLAEIKPQLEKTLASW
                     KQGNTPKKSISNAAAPKQAEVYLIDKPGAAQSFILAGTIAPPRNSPQEIALGIWNDIL
                     GGTFGGRVNMNLREDKHWSYGARTAFQFARGDSAWVTQAPVQTDKTKESLVEINKELR
                     DMFGARPISQDELNKDKDNRTLRLAGSRETMTEVG"
BASE COUNT          486 a          784 c          700 g          600 t
ORIGIN      
        1 ccaacctcgg tcatagtttc gcgcgaaccc gccaggcgaa gggttcgatt gtccttgtcc
       61 ttattgagtt catcctgcga gataggacga gcgccaaaca tgtcgcgcaa ctctttgttg
      121 atctcaacga gagattcttt cgtcttatcc gtctgtaccg gcgcttgcgt cacccaggcg
      181 ctgtcgccgc gcgcaaactg aaacgcggtg cgagcgccgt aagaccagtg cttgtcctca
      241 cgcaggttca tgtttacgcg cccaccgaac gttccgccca gaatgtcgtt ccagattccc
      301 agcgcaattt cctgcgggct gttgcgcggc ggtgcgatcg ttcccgccaa gataaaagac
      361 tgcgccgcgc ccggtttatc gatcaaatac acttcggcct gcttcggcgc cgctgcgttc
      421 gaaatgctct tcttcggcgt attcccttgc ttccaggaag caagagtctt ttcgagttgc
      481 ggtttaattt ccgccagcgt ggtgtcgccc acgatcacca gcgtagtgtt tttcggccgg
      541 taccacgtgt cgtggaattt ggcgatcgcg tcgcgcgtca atttggcgac gcccgcctcg
      601 gtcaactcca ggccgtaggg atgatccttg ccgtaaagcg ctggagtcat cacgcggccc
      661 gccatcgcgc caggggaagt cttcgcccgt tgaatcgcgg caatctgcag cttctgctgg
      721 cgcgcaaaat cttcctgtgg gaacgaagga tggagcagca catcggaaaa caaagcgagc
      781 gacggctcca gcttgctctt caacgaggag agatacaccg tcgaagtgtc cagcccggaa
      841 cctgcctcga gctctgctcc cagcctttga atctcatcgc tgatttgcag ggcgttgtac
      901 ttgtccgtgc cgctggttag cagcgaggag gacatagccg cggtgcccgg cgtggctgga
      961 ctgtccgcgg aggtgcccgc atccacgagc atcgtgaaat tcacaagcgg caattcatga
     1021 cgttccgcca gaacgacttt caaaccattc gaaagcgtca tgcgctcaaa cgccggcagc
     1081 ttcacgtttg gcgtggcgcc gacttcgggg agcttcgaac ggtccgcgcc cgcgctcgct
     1141 gccttgtatt ccggataggg cacgacttcg agttgataga gaccgtcgct gagccagcgc
     1201 cgcgccgcgt cgcgcaaatc ctgagccgtc gctttgcgca cgcgatccag cgaaatcagg
     1261 tactgttccg gatttcccgt gaatacctgg ccgcgagcca ggatgtcgga tttcccgcca
     1321 aagcctccga tgcggtcgag cccgcgcacc agattcgctt cataggctgt cttgacgcgc
     1381 tccaactctt ccgccgtcgg cccgtccttc aggaatctgt cgagctcttc ctgcacgtcc
     1441 ttgttaatgg gcgccaggct tcccccgggt ttcgccgccg caccaatact gaactggccg
     1501 ccgatttcgt tcggattgat gccgctgttc accgacgtcg cgacttgctc gtcatagacg
     1561 agccgcttgt aaaaccgcga atccttgccc gctgccagca cgtcgctcac caggtccaga
     1621 taatcggtgt cggcctggcc gtattccgga acattccaga tgttgaacac gcgcgccacc
     1681 ggcacgcggt cttgcgcctg ctggcggtgc acgccggtca ttttcgccac ccattcattc
     1741 tggtgcgcga tcgggggacc ggcgggaata tccccaaaat atttttggac cttttcgcgt
     1801 gccgttttca catcgatgtc gccggcaatc accagcgtgg cattcgaagg cccataataa
     1861 gtcttgaacc actcctgcac atccgcgagc gacgccgcgt tcaaatcctc catggagccg
     1921 atcgtcgtcc acgagtagcc atgcccggca ggataggtgt tttcggtgat cagataccgt
     1981 gagatggaat agggctggtt atcgccctgg cgcttttcgt tttgcaccac gccgcgctgc
     2041 aggtcgagtg tctccttgtt aatagtgtta taaaaatgcc ccatgcggtc ggattccacg
     2101 aacagggcat aatccagggc agatgtgggc acatcctcaa aataatttgt gcggtcggaa
     2161 ttggtagtgc cgttgaggtc cgtcgcgccc acgcgctcca tcgcatcgat gtagcgcccc
     2221 ttgaggtttt cactgccgcc gaacatcaag tgttcgaata ggtgcgcaaa cccggtcttc
     2281 cccggcttct cgttcttgga gccgacgtga taccaaatgt tgagagctac gatgggcgcc
     2341 ttgtgatctt cgtggacgag caccgtcagc ccgttcggga gcacaaattt ttcatagcca
     2401 atcttgatgt cttgccctga tgcgcttacc gccatcattg ctaacagcgc catcactagc
     2461 gaaaggctca ctcgaagctt gcggccaaga agtttcacgt gttttctctc ctatgaattg
     2521 cacatccatt ttacccggat tggattaagg ttcaaaattt tacctatggt
//
LOCUS       DASYVO010000396         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_80457, whole genome shotgun sequence.
ACCESSION   DASYVO010000396 DASYVO010000000
VERSION     DASYVO010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80457"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VGR03_13335"
     rRNA            complement(<1..210)
                     /locus_tag="VGR03_13335"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            536..2062
                     /gene="tilS"
                     /locus_tag="VGR03_13340"
     CDS             536..2062
                     /gene="tilS"
                     /locus_tag="VGR03_13340"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV1995307.1"
                     /translation="MTGGRGNNPGGRDQRSTLGPRILSSLKKQEMMRPGDRVGVAVSG
                     GADSVALLLLLLELREKLGIVLSVVHFNHKLRGKASDADEAFVAKLAAKHGLEFHSAA
                     VDVAKKAKNERANIEDAARRARYDYFRSLAESGTCTRIVVAHTADDQAETVLAHLLRG
                     TGLTGLGGIHPVAGPVIRPLLSVRRSELRPFLRARKQAWREDATNRDTKRMRARIRRK
                     LLPLLQKQFQPAIVEHLVTLAELAREDEAFLDAMAEERVRVLVQKKDERARIGIDNLL
                     GPRKKKEFYTENTERTEDTEKTEGTRALSKRMVRRIVESVKPRAGQLGAGHVEAVLEL
                     ARNGQNGSSLPLPGGIEVRRERDALVFQAVENAEVQTAHRAPREYEYKIDLANGTRGV
                     GIKELGCVFRLRVIDWPPKRVETSTSGAVLDRDRLRFPMVLRNWHPGDRLRPQGHRSA
                     HKLKRLLNEKRVSRWERDGWPVLTSDGVLVWARGFTAAAEFAADEGTREGIVIVEEQI
                     "
     gene            2059..>2263
                     /locus_tag="VGR03_13345"
     CDS             2059..>2263
                     /locus_tag="VGR03_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995308.1"
                     /translation="MSSRNGAAYSAARIARRVAALGKEVSREYKGRRLDVVVTLDRGF
                     VFAADLMRQIDAPVVCHFVREEVR"
BASE COUNT          518 a          586 c          737 g          422 t
ORIGIN      
        1 caccaccaac catgcggtca gcagtcgtat ccggtattag acccagtttc ccaggcttat
       61 cccagagtgc agggcagatt acccacgtgt tactcacccg ttcgccgctc ggaactcccc
      121 tcccttgcgg gagtgaagcc ctcgctcgac ttgcatgtgt taagcacgcc gccagcgttc
      181 gttctgagcc aggatcaaac tctcgtttga atctggttgc ttggcgaaaa aaaatcccgg
      241 gatttgcatc ccaggcagcc tgccaagcga attgttgatc gctcgaattt actcagacca
      301 tcagcgctcg aaaagctcca atggtttcga acagattctg gcacatcgta tcggttgtca
      361 aagagcaaag cccccctgtc cagcagaaag gcacatccac agcccgagaa gtatgccaag
      421 ggtaggatga aatgtcaaca acggattaac gatatccaca ggcattcaca gcggggcgct
      481 acctgcttga ggcgtggtag gatgcgccaa aagagtgatt cgtgactggt gactgatgac
      541 tggtggaaga ggcaacaacc cggggggaag ggatcaacgc tcgacgctgg ggccgcgcat
      601 cctctccagt ttgaagaaac aggaaatgat gcgcccgggc gaccgcgtgg gcgtggcggt
      661 gtccggcgga gcggattccg tggcgctgct gctgttgctt ctggagttgc gcgagaaact
      721 gggcatcgtg ctcagcgtgg tgcacttcaa tcacaaattg cgcggcaaag cgtccgatgc
      781 ggatgaagcg ttcgtcgcga aactggccgc gaagcatggg ttggagtttc actcggctgc
      841 tgttgacgtt gcaaagaaag caaaaaacga acgggccaac attgaggacg cggcgcggcg
      901 agcgcgatac gactatttcc gttcgctggc agaatccgga acctgcacgc gaatcgtggt
      961 ggcgcacact gcggacgatc aagcggagac tgtgcttgcg catctcctcc gcgggacagg
     1021 cctcaccggg cttggcggaa ttcatcccgt cgccggtccg gtcatccgtc ccttgctgag
     1081 cgtccgccgg agtgagcttc ggccttttct gcgcgctcga aaacaagcct ggcgtgagga
     1141 tgccaccaat cgcgacacga agaggatgcg cgcgcgcatc cggaggaaac ttctgccctt
     1201 gctccagaaa caatttcaac cggcaatcgt tgaacacctg gtgacgctcg cggagcttgc
     1261 acgagaggac gaggcatttt tggacgccat ggcggaagaa cgggtgaggg tgctggttca
     1321 aaagaaagat gaacgagcgc gaatcggcat cgacaatttg ctcgggccgc ggaagaaaaa
     1381 agaattttac acagagaaca cagagcgcac agaggacacg gagaagacag aggggacaag
     1441 agcactgagc aaacggatgg tgcgcagaat tgtggaaagc gtcaagcccc gcgcggggca
     1501 actcggggcg gggcatgtgg aggcggtgct ggaactggcg cgaaacgggc aaaacgggag
     1561 ttctctccct ttgccgggcg gaatagaagt acgaagagag cgagacgcgc tggtgtttca
     1621 agccgtcgaa aatgcggaag tgcaaactgc tcacagagcg ccgcgggaat atgagtacaa
     1681 gatcgatttg gcgaacggca cacggggagt gggtatcaaa gaactggggt gcgtattccg
     1741 actcagggtg attgactggc ctcccaagag agtggaaact agtacaagtg gggcggttct
     1801 ggaccgcgac cggttgcgtt tcccgatggt gttgcgcaat tggcaccccg gagaccgatt
     1861 gcgtccccag ggtcaccgaa gcgcccacaa gctgaagcgc ctgttgaatg agaagcgcgt
     1921 cagccggtgg gagcgtgacg gctggccggt gcttacgagt gacggcgttc ttgtatgggc
     1981 tcgcggtttt actgccgccg ccgaatttgc cgccgatgaa gggacccgag agggaatcgt
     2041 gatcgtcgag gaacaaattt gagcagccga aatggagcgg cgtattccgc ggcacgaatc
     2101 gccaggcgcg tggcagccct agggaaagaa gtttcgcggg aatacaaggg gcggcggctg
     2161 gatgtggtcg taacgctgga tcgcggtttt gttttcgcgg cggacttgat gcggcagatt
     2221 gacgcgccgg ttgtttgtca cttcgtgcgc gaagaggtgc ggg
//
LOCUS       DASYVO010000397         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_125115, whole genome shotgun sequence.
ACCESSION   DASYVO010000397 DASYVO010000000
VERSION     DASYVO010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125115"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1657
                     /gene="acnA"
                     /locus_tag="VGR03_13350"
     CDS             <1..1657
                     /gene="acnA"
                     /locus_tag="VGR03_13350"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEV1995309.1"
                     /translation="QVALVEAYCKEQMLFRTDQTPAPLFNDTLELDLGTVEPTVAGPK
                     RPQDRVALRQAKASFTKVVEGTPAKQVAVRNNGDSFELSSGAVVIAAITSCTNTSNPS
                     LMLGAGLLAKKAVERGLHVKPWVKTSLAPGSKVVTDYLQTSGLMPYLEKLNFHLVGYG
                     CTTCIGNSGPLPDAIGAAIKDNNLIAVAVLSGNRNFEGRIHPLVRANYLASPPLVVAY
                     ALAGRMDLDLTTEPLGNDPAGKPVYLKDIWPTPQEIESTVRSSVSSSQYSKHYSEVFE
                     GDAHWKSMPIPQGDLYKWDPKSTYIKLPPFFENMPKAPPALADIHGAKVLAILGDSVT
                     TDHISPAGSIPVESPAGKYLIANGVKPHEFNSYGARRGNHEVMMRGTFGNIRLRNQLA
                     PGTEGGWTLFLPDGEKMSIYDAAVKYREAGVPLVVLAGKEYGSGSSRDWAAKGTRLLG
                     VRAVIAESYERIHRSNLVGMGVLPLEFKPGENRESLGLTGHEIFEIEGVASLAPKKQI
                     PVHAKSGDGTVKTFSVIARADTPEEVSYYHHGGILQYVLRQML"
     gene            1840..>2229
                     /locus_tag="VGR03_13355"
     CDS             1840..>2229
                     /locus_tag="VGR03_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995310.1"
                     /translation="MLKQLASLALLLIVSAAVAPAQQTPSSPQVILIRAGKLVDVNAG
                     RVLTNQMLLVRGAKIEAIGEHLAVPAGATVIDLSKMTVLPGLIDCHTHLADLGYSEPL
                     LVLQKSAAESAYAVIPNARVTLLGGFTT"
BASE COUNT          447 a          705 c          623 g          454 t
ORIGIN      
        1 acaggtcgct ctggtcgaag cttactgcaa ggagcagatg ctctttcgca ccgaccagac
       61 gcccgcgccg ctctttaacg acacgctgga attggatctg ggaaccgtcg agcctacagt
      121 cgctggtccc aagcgtccgc aggatcgcgt tgcgctgcgc caggccaagg cttcttttac
      181 gaaagtcgtg gaaggaacgc cggccaagca ggttgccgtc cggaacaacg gcgactcatt
      241 tgagctttcg agcggcgccg tggtcattgc cgccatcacc agctgcacca acacttcgaa
      301 cccctcgctc atgctgggcg cgggcttgct ggcgaagaag gccgtcgaac gcggcttgca
      361 cgtcaagccc tgggttaaga ccagcctcgc gccgggttcg aaagtcgtga ccgattatct
      421 gcagacctca ggcctgatgc cctacctcga gaaactcaat tttcacctgg tcggctatgg
      481 ttgcacgacc tgcatcggca acagcggtcc actgccggac gccatcggcg cggccatcaa
      541 ggacaacaat ttgatcgccg tcgccgtcct cagcggcaat cgcaattttg agggccgcat
      601 tcatccgctg gtgcgcgcca attatctggc ctcgcccccg ctggtcgtcg cttacgctct
      661 cgcggggcgc atggaccttg acctcaccac cgaacctctt ggcaacgatc ctgcaggcaa
      721 accggtctat ctgaaagaca tctggccaac gccgcaggag atcgaatcaa ccgttcgttc
      781 ttctgtttcg tcgtcgcaat acagcaagca ttacagcgaa gtgttcgaag gcgacgcgca
      841 ttggaagtcc atgcccattc cgcagggtga cctctacaag tgggacccga aatcgacgta
      901 catcaagctg ccaccgtttt tcgagaacat gccgaaggcg cctccggcgc tggcggacat
      961 tcacggcgcg aaagtgctgg ccattcttgg cgacagcgtc accaccgatc acatttcgcc
     1021 tgccggctcg attcccgtcg aatcacccgc tggcaaatat ctcatcgcca acggcgtgaa
     1081 gccgcatgag ttcaactcct acggcgctcg ccgcggcaac cacgaggtca tgatgcgcgg
     1141 cacgtttggg aacattcgcc tgcgcaacca gctcgctccg ggcaccgaag gcggctggac
     1201 cctttttctt ccggatggcg agaagatgtc gatctacgac gccgccgtaa aatatcgtga
     1261 ggctggtgtg ccgctggtgg tcctcgccgg aaaagaatac ggctccggct cttcgcgcga
     1321 ttgggcggcc aaaggcacgc ggctgctggg tgtgcgcgcg gtcattgcgg aaagctacga
     1381 gcgcatccac cgcagcaacc tcgtcggcat gggtgtgctc ccgctcgaat tcaagccggg
     1441 agaaaaccgt gaatcgcttg gcctgacggg tcacgaaatt ttcgaaatcg aaggcgtcgc
     1501 gtcacttgcg ccaaaaaaac aaatcccggt gcatgctaaa tcgggggatg gaacggtaaa
     1561 gaccttttcg gtgatcgcac gtgcagatac gccggaggaa gtctcctact accatcacgg
     1621 cggcattctg caatacgtct tgcggcagat gctctagcac tcgtcaattc tcctggccca
     1681 caagcccccg gatgtccccg tccggggact tttttttggg caagtgcgtt atgctcctca
     1741 aaattgaagt aagccttgcc gcggagtatg atgcgcaggc gcaatctgca taccacggcg
     1801 tacctctggc ctgtatcttg cccgcgcgaa aggacatctg tgctcaagca actcgcctct
     1861 cttgcgttgc tgctgatcgt ttctgcggcg gtcgctccgg cccagcaaac gccatcttct
     1921 ccgcaggtca tcttgattcg tgctggaaaa ctcgtggacg tgaacgcggg ccgagttctg
     1981 acgaatcaga tgcttctggt gcgtggcgcc aaaattgagg ctatcggcga acatttggcg
     2041 gtcccggccg gggccactgt gatcgatctg tcaaaaatga ccgtcttgcc cggactgatc
     2101 gattgccaca cccacctggc cgacctgggc tactctgagc ccctcctcgt gctgcaaaaa
     2161 tccgccgccg agagcgccta tgccgtcatc cccaacgcgc gcgtcacact cctcggtgga
     2221 ttcactacc
//
LOCUS       DASYVO010000398         3205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_80496, whole genome shotgun sequence.
ACCESSION   DASYVO010000398 DASYVO010000000
VERSION     DASYVO010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80496"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VGR03_13360"
     CDS             <1..291
                     /locus_tag="VGR03_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995311.1"
                     /translation="RHHVTVDELREYWSPISPMPYVERGMGAGRNTFMVYGKYDPTML
                     PELTRQMLDSLRRHGADPRTLELPCGHYSLELAPFSYIAGYRMLTFFLQSLA"
     gene            complement(374..613)
                     /locus_tag="VGR03_13365"
     CDS             complement(374..613)
                     /locus_tag="VGR03_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995312.1"
                     /translation="MQNRICACVSSPRCPKSPLVNDGKLTHIDHVVTVKAFANDVLQR
                     ELTFDEAYLRSWDDSNLRAVHRGCNYDRNVGRKLP"
     gene            complement(866..1186)
                     /locus_tag="VGR03_13370"
     CDS             complement(866..1186)
                     /locus_tag="VGR03_13370"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1995313.1"
                     /translation="MKSDRHRQIVGRRVRKSAVYRKTFERTLQRIDLALLVREMREKA
                     EFTQTELAKRVGTTQSVIARLEDAEYAGHSLTMLERIAGACGVGLRLHAEKKPNFERE
                     VALV"
     gene            1314..1868
                     /locus_tag="VGR03_13375"
     CDS             1314..1868
                     /locus_tag="VGR03_13375"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEV1995314.1"
                     /translation="MDKLLLFVFGPLVFAAALLVIATGIRRALAKFRSRPAPDQIKDA
                     YDAYLHRLLNPQPDVVERELGKLLPERLLQLYQDKIAIQSAGFQLEKPGKKRWRAERW
                     PVYCFEPLDNEALNELPYEEKLGPGFCFATTGRGCWYWIAASDQRAKDSPVIFLDYDG
                     SGSHGETVADSLDEFLNWPRLPLK"
     gene            complement(2415..>3205)
                     /locus_tag="VGR03_13380"
     CDS             complement(2415..>3205)
                     /locus_tag="VGR03_13380"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEV1995315.1"
                     /translation="YAGAATTARNLAHLNEGSQIRESHRASEKDPRVQDAYSLRCTPQ
                     VHGAVRDSLGQAREMAAVELNSATDNPLVFVRDANNGDIISGGNFHGQPLAMAADQAA
                     IAIATLGGIVERRIEQMTNPLTSMLPAFLTPEPGLNSGFMIAQVTAAALTSENKTLAT
                     PHSVDSISTSGNQEDYVSMGMSGARRLGRMLVNLRHTIAIELLCACQGIDLLAPLQTG
                     TLAKKAYEAVRLKSRKVTEDRTLAVEIEVVSALVAEGAFSALLR"
BASE COUNT          637 a         1077 c          770 g          721 t
ORIGIN      
        1 cgccatcacg taacggtcga cgaactccgc gaatactggt ctcccatcag cccgatgccc
       61 tacgtcgaac gcggtatggg cgctggccgc aatacattca tggtttacgg caagtacgac
      121 cccaccatgc tccccgagct cacccgccag atgctggact ccctccgccg ccacggcgcc
      181 gatccccgca ccctcgaact cccctgcggc cactactccc tcgagctcgc ccccttctcc
      241 tacatcgccg gctaccgcat gctcaccttc ttcctccaat ccctcgcctg aagggccagg
      301 cgtctgaagc cggtaagcct cttggcggac gatcaaagac gccgattctg ggattgtggg
      361 tgttatggcg attttacggc agcttccgtc caacgtttcg gtcgtaattg catccgcgat
      421 gaaccgcgcg aagatttgaa tcatcccacg accggagata cgcctcatca aaggtcagtt
      481 cgcgttgaag aacatcgttg gcgaatgctt tcaccgtcac gacgtggtca atgtgcgtga
      541 gcttgccgtc gttgactagc ggcgactttg gacagcgagg agaagagacg caagcacaga
      601 tgcggttttg catctctatc agccggggcc gttcttttgc aagccggaaa ccttgcggtc
      661 gcagcctgtt cgaacgccaa ttccgaagca gctgaacaat catgttctta tcgcaatcaa
      721 tcatcattgc acacccccaa gcagcgcgac actttacggg aagagactac gctttgctgc
      781 tcagttttgt ctccaacatc cccatcccat cgaccaacgc gggtgccccg cgtggcgcct
      841 ttgcgccttg gatctttcgt ctcgttcaca caagcgccac ctcgcgctcg aaattcggct
      901 tcttctccgc atgcagcctg agccccaccc cgcaagcccc agcaatccgt tccagcatcg
      961 tcagcgaatg ccccgcgtac tccgcgtctt ccagccgagc aatcaccgac tgtgtcgtcc
     1021 ccaccctctt ggccagctcc gtctgcgtga actcggcctt ttcccgcatc tctcgaacca
     1081 gcagcgctag gtcaatccgt tgcaacgtac gctcaaacgt cttgcgatac acagcgctct
     1141 tgcgcacccg tcgcccaacg atctgcctgt gcctgtcgct cttcatagcc caaactcctt
     1201 cctatttgaa gtatcgcata tttgcgatag tcaagtaggt gcagtgcgct tcgcaagggc
     1261 cgcttgctca tgagaatcaa tcctttcgct cccctctgcg ctatcatcag gtcgtggaca
     1321 agcttctgct ctttgtgttc ggccctctcg tcttcgccgc cgcgctgctc gtcatcgcca
     1381 cggggattcg ccgtgcgcta gcaaaattcc gttcccggcc agcgccagat caaatcaagg
     1441 acgcttacga cgcatacctg caccgtttgc tgaatcccca gcccgacgtc gtcgaacgcg
     1501 aattgggcaa attgctcccc gaaaggcttc tgcagttgta ccaggacaaa atcgccatcc
     1561 agtcggccgg cttccaactc gagaagcccg gcaagaaacg ctggcgggcc gaacgctggc
     1621 cggtttattg tttcgagccc ttggacaacg aagccctcaa cgagttgccc tacgaggaga
     1681 agttggggcc cggcttttgc tttgcaacca caggccgtgg atgctggtac tggatcgccg
     1741 cctccgacca aagggcaaaa gattcccccg tcatcttcct ggactacgac ggcagcggct
     1801 cccacggaga aaccgtcgcc gacagcctcg acgaattcct gaattggccg cgtcttcccc
     1861 tgaaatgagc tttcccgcaa tctccgacta tcgccacaca caaccggtcg ccagaacctc
     1921 ttgactttcc atactttctt cgttactatc ctttccaggt aagtgtgcgg gaattcttcg
     1981 attgcggcgg ttcgttcgcc gctcttttta ttttcactgc gccagcaatt ctatccggga
     2041 acgccatctc tgctccatcc cgcgggttca gcgggatgaa tctcctgatt ggcgttgggt
     2101 attctgcgac agtgacctcg ctcttctact tcggatagcg acctaagtca tttagaataa
     2161 acacttacaa aaatgtacca gaaatgcgcc tctaactccc tttagaatca acacttatag
     2221 aaatgcccgc aagtgttgat tctaaaccac ttacataaac tctaagtcct ttagatgcaa
     2281 cacttacaaa aaacataggg gggtgggggt attatggtta actagaaagc cctttggagt
     2341 gcggcagctc gctgccgcct ttcttcctgc cggggcgtcc ctttccggcc gtcaggctgt
     2401 actaagaacc cctctcaccg cagcaaagct gaaaacgcac cctcggccac caacgccgac
     2461 accacttcaa tttcgaccgc cagcgtacgg tcctcggtaa ctttacgcga cttgagccgc
     2521 acggcttcgt atgctttctt tgccagtgtc ccagtttgca gcggcgccag caaatcgatc
     2581 ccttggcatg cgcaaagcaa ttcgatcgca atcgtatgcc gcaaattgac cagcatgcgc
     2641 cccagccgcc gcgctccgct cattcccatc gaaacgtaat cttcctgatt cccgctcgtg
     2701 gaaatggaat cgacggaatg cggtgtagcc agcgtcttgt tctcgcttgt cagcgccgcc
     2761 gccgtcacct gcgcgatcat gaatcccgaa ttcagccccg gctccggggt cagaaacgcc
     2821 ggcagcatgc tggtcagcgg attggtcatc tgctcgatgc gccgttcgac aatccccccg
     2881 agcgtcgcaa ttgcgatcgc cgcttgatcc gccgccatcg ccagcggctg tccgtgaaaa
     2941 tttcccccgc tgataatgtc gccgttgttc gcgtcgcgca caaaaacgag cggattatcc
     3001 gtcgcgctgt tcaactccac cgccgccatt tcccgcgcct gccccagcga atcgcgcacc
     3061 gccccgtgta cctgcggcgt gcaacgcagg ctatacgcat cctgcacgcg cggatctttt
     3121 tccgacgccc gatgcgattc gcgaatctgg ctgccctcat tcaagtgcgc gagattccgc
     3181 gccgttgtcg ccgctcccgc atacg
//
LOCUS       DASYVO010000399         2661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_39712, whole genome shotgun sequence.
ACCESSION   DASYVO010000399 DASYVO010000000
VERSION     DASYVO010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39712"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VGR03_13385"
     CDS             <1..383
                     /locus_tag="VGR03_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995316.1"
                     /translation="EQALHGRLISCDVWSGHMQSIRVARNLECRACGRRDFLYLQGEA
                     QPHVTMCGRDSVQIHERSRSLDLFALGVRLAPLVEDVRQNDFLLRFRVAPYEMTVFAD
                     GRAILKGTKDPAVARSLYARFIGA"
     gene            complement(612..848)
                     /locus_tag="VGR03_13390"
     CDS             complement(612..848)
                     /locus_tag="VGR03_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995317.1"
                     /translation="MRRETALGKAPQERQREIMQFITENGERLARVATSGLHLTDDLK
                     ARILSTFLTLMNLRENLDRANMRSSFGRSSHPLR"
     gene            1169..1618
                     /locus_tag="VGR03_13395"
     CDS             1169..1618
                     /locus_tag="VGR03_13395"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995318.1"
                     /translation="MSTGKTFRQGQILRLVSDQRIANQEELRRRLAVQKMRVTQATLS
                     RDLQELRLVKTQEGYRPATALPEESTPLPPLARALGEFLVDIRPAENLLVLKTPPSGA
                     QPLAAAVDGAKFPEIAGTIAGDDTVLIITPNKKSRASLQKKIEALVK"
     gene            1615..2646
                     /gene="argC"
                     /locus_tag="VGR03_13400"
     CDS             1615..2646
                     /gene="argC"
                     /locus_tag="VGR03_13400"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEV1995319.1"
                     /translation="MRDPRQIAVVGVTGYTGLELARILLRHPALHAPVFYLRETSGAK
                     CLAELFPQFRGWGEAPLRPLSVDAISSSHAGTAFLATPHEVSTELAPALIEAGLRVVD
                     LSGAFRFHAAETFTSWYKLPAPHADWLGEAVYGIPEIYANEIAEARLVANPGCYATSV
                     ILALRPLTQAGWIASDSTVVCDCKSGASGAGKETRRDLQFVEVDENFKAYGLFTHRHT
                     PEILEHTGLSETQVVFSTHLLPLARGILSTIYVTLDKLQAAADVEALYRKFYAGRPMV
                     RIWPAGTLPELQHVAHTNFCDIGFAMDKAGRRVVIVSCLDNLGKGAAGQAVQNFNHML
                     GIEEQTSLQ"
BASE COUNT          513 a          815 c          744 g          589 t
ORIGIN      
        1 gtgaacaggc tcttcatggg cgcctgattt cctgcgatgt gtggagcggc cacatgcaat
       61 ccattcgcgt ggcgcgcaac ctggagtgcc gcgcctgcgg gcggcgcgac ttcctctatc
      121 tccagggcga ggcccagccg cacgtcacca tgtgcggacg cgattccgtc caaattcacg
      181 aacgcagccg gagcctggat ctctttgccc tgggagtacg cctggcgccc ctcgtcgagg
      241 atgtgcgcca gaatgatttt cttcttcgtt tccgcgtggc tccgtacgaa atgactgtct
      301 tcgccgacgg acgcgccatc ctgaagggta caaaagaccc tgccgtggcg cgctctctct
      361 acgctcgttt catcggcgcc tgattttgct ggcctccatg ctagagtttg gtttcgaaac
      421 ctctttcacg ccgggagact gcggcaattt gggctggcta aagcagagtt tcgaactaag
      481 cgcgggagaa ccggtaacat tacagtcttt ttctttcttg ctaagaacta agcactaagc
      541 actgatgact atgcacgagc aaaacgccaa tcgaattgcg gtattgaaat ggtttctggt
      601 tgtttgcgag tttaacgaag cgggtggctg gaacgcccga acgagctccg catgtttgcg
      661 cggtcgaggt tttcccgcaa attcatcagc gtaagaaacg tgctcagaat ccgcgctttc
      721 aggtcgtctg tcaggtgcag cccgctggtc gccacgcgcg ccaggcgttc cccgttttcc
      781 gtgatgaact gcatgatctc acgctgccgt tcctgcgggg ctttgccgag cgccgtctcc
      841 cgtcgcattt cgccgcagct cgtttccagc gagagagtga agtgcaaaac gcagagccgc
      901 tgctcggcta ttgccggcgg cgcgtcgttc ttgcatcccg agatttggca aggcgttccc
      961 atggggcccg tggcgcaaca cgataggtaa catccccggc ccggaaaatc cagtgtacct
     1021 cgcgccggtg ctgcgaatct tacatgatcc gctttttccg tctctcgcgc catcggtggt
     1081 gcccttgccc aaaaagtctt caaattacat tgacatgggt atacaatcac atgtataaaa
     1141 atacagagac gcagtggttg gacacaaaat gagcactggc aagacatttc ggcagggtca
     1201 gatccttcgc ttggtctccg accagcggat cgcgaatcag gaagagctgc gccgccgcct
     1261 ggccgtgcag aaaatgcgcg tgacccaggc caccctttcg cgtgacctgc aggagctgcg
     1321 gctcgtaaag acgcaggaag gctacaggcc ggcgactgcc ttgcctgagg aatccacgcc
     1381 cctacccccg ctggcgcgcg cccttggtga attccttgtc gacatccggc ctgcggaaaa
     1441 tttgctcgtt ttgaaaactc cgcccagcgg cgcgcagccc ctggccgccg ccgttgatgg
     1501 cgcgaagttt cccgaaattg ctggaaccat cgccggtgac gataccgttc tcatcatcac
     1561 accgaacaag aaatcacgcg catctctaca aaaaaagatt gaagccctgg tgaaatgaga
     1621 gatccccgcc aaattgcggt cgtgggtgtc accggctaca ccggtctgga gctggcgcgg
     1681 atccttctgc gccaccctgc gctccacgcc ccggtctttt acctccgtga aacgagtggc
     1741 gcgaaatgcc tcgcggaact cttccctcag ttccgtgggt ggggtgaagc tccgctccgc
     1801 ccgctctccg tggacgccat ctcttcgagc cacgcgggca ctgcatttct cgcgaccccc
     1861 catgaggttt ctacggaatt ggcgccggcc ttgattgaag cgggtctccg cgtcgtggat
     1921 ttgagcggcg cattccgctt ccacgctgcg gaaactttta cttcctggta caaactgccc
     1981 gcaccgcatg cagattggct cggcgaagct gtctacggca ttcctgagat ctatgcaaac
     2041 gagatcgccg aggctcgctt ggttgctaat cccggttgtt atgcgaccag cgtgattctt
     2101 gccttgcgcc ccctcacgca ggccggatgg attgcttcgg attccactgt cgtttgcgac
     2161 tgcaagtccg gcgccagtgg cgcgggcaag gaaacccggc gcgatctcca gttcgttgaa
     2221 gtggacgaaa atttcaaggc ctacggcctc ttcacgcacc gccacactcc ggagattctt
     2281 gagcacaccg ggctgagcga aacgcaagtg gtcttctcga cgcatctgct gccgctcgcg
     2341 cgcggcattc tttccacaat ttacgtcacg ctggataaac ttcaagccgc tgcggacgtc
     2401 gaggccctgt accgcaagtt ttacgcgggg cgtcccatgg ttcggatctg gcccgccggc
     2461 acgctgcccg agctgcaaca cgtcgcccac accaattttt gcgatattgg ttttgctatg
     2521 gataaggcgg gccgtcgcgt cgtgatcgtc tcctgcctcg acaatcttgg aaaaggcgcc
     2581 gccggccagg ccgtgcagaa tttcaatcac atgctgggaa tcgaggagca gacatcactt
     2641 caatgagaat cgttatcaag t
//
LOCUS       DASYVO010000400         2723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_94959, whole genome shotgun sequence.
ACCESSION   DASYVO010000400 DASYVO010000000
VERSION     DASYVO010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94959"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1713
                     /locus_tag="VGR03_13405"
     CDS             <1..1713
                     /locus_tag="VGR03_13405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV1995320.1"
                     /translation="PPQDAPAKTDSTANPAPAPPLGENQEVSSHDTPTTFKVRVNLVL
                     VRVVVRDLHGKIVPNLKKEDFQLYDNRKLQTISSFSVETPETRTASAVASTAAEGSSS
                     SADAAGGKAVVLPQRFVSMVFDDVHLSMADAVFVRDSATRFFEALAASDRVSLSTTSG
                     QLTQEFTDDHDKLAKALLGIVPHSLTSHSFHDCPDVSYYQADLIENRSDQQALAVATD
                     EALQCAFGGDTRMIASAQSLAQSAASRTLSQGDNETQYAYRHLEDVVRRLASMPGQRV
                     LVLVSPGFITTTMQSEASEMVDRATRANIVINTIDARGLYAPDVMGDIADPPRDTFRT
                     VGYKTSYRVAAQFAQEEVLAQLANGTGGKFFHNRNDVDEAMREAGAAPAYSYLLGFSP
                     QNLKIDGRFHALKVALTSKEKFEIQARHGYFAPKTLTDPVEATKLEIQEALFSQEEIR
                     DLPVELQTQFFKKDDAQARLAVLTHFDVKGIHFRKLLGRNNDQLTIVTGIFDENGNFV
                     TGLSKVVEMNLLDTTYTRLSRSGFTVKTSFDVKPGTYLVRLVVRDAVGAQMAARNGAV
                     VIPY"
     gene            1740..>2723
                     /locus_tag="VGR03_13410"
     CDS             1740..>2723
                     /locus_tag="VGR03_13410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV1995321.1"
                     /translation="MNTLTNLKRTIQLATWLAAGVMCLTPSPSRGQGQPAPPLPQAAS
                     PAAEQQPVVVIKKESKLVLVDSVVTDKKGNYIRDLTQNDFKVFEDNKEQPVSTFSTGA
                     DAATQANGQRRYLILFFDNSTMAAPDQIQARSAATKFIAANAGPDRLMAVVDFGGSLR
                     IVQNFTANADVLRAAVSGVKNSSVDPNAPAPGGPVTVASTGLSSLGNAEADFGARSML
                     LAVRSLAKNLRAVPGRKMVVLFSGGFPLTQENQSELTATIDACNKSNVAVYALDARGL
                     VATAPGGSASKKTSIGKTHSVSNHVPVRKTSPRPHLLLAAYPAMAMPDPQRP"
BASE COUNT          596 a          799 c          799 g          529 t
ORIGIN      
        1 ccaccacagg atgcacccgc aaagacagat tcgacggcga atcccgcgcc cgcgcctcca
       61 ttgggcgaaa atcaggaagt ctccagtcac gatacgccga ccaccttcaa ggtgcgcgta
      121 aacctggtgc tggtgcgcgt agtggttcgc gatttgcacg gcaaaatcgt ccccaatctc
      181 aagaaggaag actttcagct ctacgacaac cgaaaactgc agacgatctc ttcttttagc
      241 gtggagacac ccgaaacgcg cacggcctcg gcagttgcat ccacagccgc ggagggttct
      301 tcgtccagtg cggatgccgc cggcggcaag gccgttgttc tgccgcagcg atttgtctcc
      361 atggtcttcg acgacgtgca cctctcgatg gccgatgcgg tgttcgtccg cgattcggca
      421 actcgattct ttgaagcttt ggcggcgagt gaccgcgttt cgctgagcac gacctccggg
      481 cagttgacgc aggagttcac ggacgatcat gacaaacttg caaaagctct gctcggaatc
      541 gtgccgcatt ccctgacgag ccacagtttt catgattgcc ctgacgtgag ttattaccag
      601 gccgacctga tcgagaacag gtccgaccag caggcgctcg ccgttgccac cgatgaagcg
      661 ctccaatgcg cgttcggcgg ggacaccagg atgattgcgt cagcgcagag cctggcgcag
      721 tccgcggcaa gccgcacgct gtcgcaagga gacaacgaga cgcagtatgc ctataggcat
      781 ctggaagacg tcgtcagacg cctggcaagc atgcccggtc aacgcgtgct cgttctggta
      841 tcgcctggct tcatcaccac gaccatgcag tccgaagctt ccgaaatggt cgatcgcgcg
      901 actcgcgcca acattgtgat taataccatc gatgcgcgcg gcctctacgc cccggacgta
      961 atgggggata tcgccgatcc accaagagac accttccgca ccgtaggcta caaaacctct
     1021 tatcgcgtgg cggcgcaatt cgcgcaagag gaggtcctgg cgcagttggc taatggaacc
     1081 ggtggcaaat tcttccacaa ccgcaacgac gtggatgaag ccatgcgcga ggcgggcgcc
     1141 gcgccggcgt attcctactt gctcggattc tcgccgcaga acctgaaaat cgacggacgg
     1201 tttcacgcgc tgaaggtcgc acttacaagc aaggagaaat tcgagatcca ggcaaggcac
     1261 ggttactttg cgccgaagac gctcaccgac cccgtcgaag cgacaaaact ggaaatccag
     1321 gaggccttgt tttcacaaga ggagatccgc gacctgccgg tggaattgca aacgcagttt
     1381 ttcaagaagg atgacgccca ggcgcgcttg gcggtgctga cgcactttga cgtgaagggc
     1441 atccacttcc ggaaattgct ggggcgcaac aacgatcaat tgacgatcgt caccgggatt
     1501 ttcgacgaaa atggaaattt tgtcaccggc ctttccaagg tcgtggagat gaacttgctc
     1561 gacacgacgt acacccggtt gagccgctcg gggttcacgg tgaaaaccag ttttgacgtg
     1621 aagccgggca cctacctcgt gcgtctggtg gtgcgagacg ccgtgggtgc gcagatggca
     1681 gcacgcaacg gtgcagtggt cattccttat tgaagttaag tgattgtggt gggggaaata
     1741 tgaatacgct gacaaacctt aagcgaacaa ttcaattggc cacatggctg gcggccggag
     1801 tgatgtgctt gactccttct ccttcacgag ggcaggggca acccgctcca ccacttccgc
     1861 aggcggcctc accagcggcg gagcagcagc ccgtggtggt aatcaaaaaa gaatcaaagc
     1921 tcgtgctcgt cgattcggtg gtgacggaca agaagggcaa ctacatccgc gacctgacgc
     1981 agaatgattt caaagttttc gaagataaca aggaacagcc ggtttccact ttttctaccg
     2041 gcgccgatgc ggccacccag gcgaacgggc aacgccggta tctgattttg tttttcgata
     2101 attccacgat ggcggcgccg gaccagatcc aggcgcgcag cgccgctacc aagttcatcg
     2161 ccgcgaacgc cggccccgat cgattgatgg ccgtcgtgga tttcggtggc tcgctccgca
     2221 tcgtgcagaa tttcacggcg aacgcggacg tgcttcgcgc ggcggtatcg ggagtgaaga
     2281 attcctcggt cgatccaaac gcgcccgcac cgggcgggcc cgtcacggta gcgtcgacag
     2341 gtttatcgtc actgggcaat gccgaggcgg attttggggc ccgcagcatg ctgctggcgg
     2401 tgcgcagcct ggcgaaaaat ctccgcgccg tccccggccg aaaaatggtg gtgctgttct
     2461 cgggagggtt cccgctgacg caggaaaacc aatcggagct aacggcaacc attgatgctt
     2521 gcaacaagtc gaatgtggcg gtctacgcgc tggatgcgcg cggtttagta gcgaccgcac
     2581 caggcggaag cgccagcaag aaaacttcga ttgggaagac tcattccgtt tcaaatcatg
     2641 tgcccgttcg gaaaacttca ccgcgtccac accttctcct cgctgcctat ccggcgatgg
     2701 ccatgcccga tccgcagcgt ccg
//
LOCUS       DASYVO010000401         2444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_80498, whole genome shotgun sequence.
ACCESSION   DASYVO010000401 DASYVO010000000
VERSION     DASYVO010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80498"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..1265
                     /locus_tag="VGR03_13415"
     CDS             285..1265
                     /locus_tag="VGR03_13415"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1995322.1"
                     /translation="MNRRDAVLALLALGAAPFAAVAQQSGKVWRIGVLRPAPDDAGFR
                     QNFDPFRQALRKLGFTEGANLAIEYRVRPGTAVEILALADDLVRSKVDAIFAVASVAV
                     SAAAKSTTSIPIVALDLESDPIAEGFAANLARPGGNVTGLFLDFPGMSGKWIQLLKES
                     VPKLARVAVLWDPATGPTVFKGAEEAASSMRLQLLRLEVRGPADFARAFQSAAAQKAQ
                     ALLVLSSPVFNSARKELAESAMKHRLPTMMPFPGFADDGGLMAYGPHLASLYGHAGGI
                     VAKILKGARPGEIPIERPRRFELAINLKTAKALGITIPQSVLLGADRVIE"
     gene            1516..2070
                     /locus_tag="VGR03_13420"
     CDS             1516..2070
                     /locus_tag="VGR03_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995323.1"
                     /translation="MKIARYKLARSLFAVAVTVGLTISGMSNGSAQEKQKYFFKTPAG
                     ITKYAQQHVIDVGDVPGHQIRVYELHSKFGTDAPVYDGVKVVEAWTRAASDYTNGSGR
                     NSGYGVALLANGDKIFSVVDVISQSKAGPDGAKKTTFTSVSRFVGGTGKFKGIRGTLM
                     GTGTTDFKTGTSDTVTEGEYWIEK"
BASE COUNT          486 a          691 c          745 g          522 t
ORIGIN      
        1 gtggcgcgct catccgccgc catcagccaa gagtaacccg ccacgtccgc agccaggatt
       61 gccgctaacc gctgctttag ttcagtaccg ctcacttgta tcggcctctc cccgaggcgt
      121 ttgcgggtgc atgttacccc cgacgtggtg gggtgctcga atgaccgctt ccgacccaaa
      181 gcggacattc gggcatagac tgattttcta ctaggattgc acggggcgct ccgcctctcc
      241 gatcccggtg ctaacgcttt gtcgtagcaa ccgaagggga tcgaatgaac aggcgtgacg
      301 cggtactcgc actcctcgcg ctcggcgcgg cgccgtttgc cgccgtggcg cagcagtcag
      361 gaaaagtttg gcgcatcggg gtgcttcggc ccgcaccgga cgatgcgggt ttccggcaga
      421 actttgaccc gttccggcag gcgcttcgca aactcggatt cacggagggg gcaaatctgg
      481 cgatcgaata tcgggtgcga cccgggacgg cggtggaaat actcgccctc gcggacgatc
      541 tggtgcgctc caaggtggat gcgatttttg ccgtcgcttc ggtggcagtg agcgcggccg
      601 cgaaatcgac cacgtcgatt cccatcgtgg cactcgatct ggagtcggat cccatagcgg
      661 agggcttcgc cgcgaacctg gcccggcccg gtggaaacgt caccggcctg tttctcgatt
      721 ttcccgggat gagcggcaag tggatccagc tgctgaagga atcagtgcca aaactagccc
      781 gggtcgccgt gctctgggac ccggccactg ggcccaccgt cttcaaggga gcggaggaag
      841 ctgcatcgtc gatgcggctg caactcctcc ggctggaggt gcgcggcccg gcggattttg
      901 cgcgcgcgtt tcaatcggcg gcagcccaga aggcgcaagc gcttctagtg ctgtcttcac
      961 ccgtgttcaa ctcggcccgc aaggagctcg cggagtcggc catgaagcac cgattgccga
     1021 ccatgatgcc tttccccggt tttgccgacg acggtgggtt gatggcgtac gggccgcacc
     1081 tcgcgagcct gtacggccac gcaggaggca tagttgcgaa aattctgaaa ggtgcccggc
     1141 cgggcgagat tcctatcgag cggccgaggc gtttcgagct ggccatcaat ctgaagaccg
     1201 ccaaagcgct cggcatcacc atcccgcagt cggtgctgct gggcgccgac cgggtgatcg
     1261 aatgaccgct attggccgaa agcggtcatt ggcgaatggc tggagatcta ttgggagttc
     1321 ttgattgctg cttttggggc aggcgcctag aattgccttc aacgtagtca aaaaccagcc
     1381 gatgcgctgg gcgttcattt tgttttcggc cagcgggccc gtccttcaaa gcacatctgt
     1441 gctacccgaa cgtcgtttct gtgtgctgcc tgactgggtg gcgctagaaa ctttcctgaa
     1501 tcaaaagggg ggaatatgaa gatcgctcgc tataagctag ctcgttcgct gttcgcagtt
     1561 gcagtgaccg tcggattgac gatttccgga atgtcgaatg ggtcggccca ggaaaaacag
     1621 aagtattttt ttaagactcc tgcaggaatt accaagtacg cacaacaaca cgttatcgac
     1681 gtcggcgatg ttccgggtca tcagattcgg gtctacgagc tccactcgaa gttcggcaca
     1741 gatgcaccgg tgtatgacgg cgtcaaggtt gtggaggcgt ggactcgggc cgcctcggac
     1801 tataccaatg gaagtggccg aaactccggc tacggggttg ccctgctggc gaatggcgac
     1861 aaaatctttt cggtcgtaga cgtcatctct caaagcaaag ctggtcccga tggggcaaag
     1921 aaaaccacat ttaccagtgt atccaggttt gtcggcggca cgggcaaatt caagggaata
     1981 cgcggcacct tgatgggcac aggtacgacc gatttcaaga cgggaacgag cgacacggtg
     2041 acagagggtg aatactggat cgagaaatag atcgaatgtc gcttggagat gccccggcct
     2101 tggccagggc atcttttcat ttgcggtgaa gagccgatct ccaacatgcc gaggccgtaa
     2161 cagatcgcaa ggatctcgga gattggcaaa tgcgttcaga caacgtcgcg atctcaaatg
     2221 tgtgcctttt tcccctagcg gcttgtaagt ccaatgtccg ctttcgaccc aaagcggtca
     2281 ttcagggctt agactgacct ctgactaggg ttgactaagc tttccgccta ctaatcccgg
     2341 ttctcgcgct ggctcccaac gaccagaggg gatcgaatga accgccaaac aaggttcttc
     2401 tttttcgcgc tccgccttgg cgctttaatt ggcattgtgt tcgg
//
LOCUS       DASYVO010000402         6337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_233818, whole genome shotgun sequence.
ACCESSION   DASYVO010000402 DASYVO010000000
VERSION     DASYVO010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233818"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VGR03_13425"
     CDS             complement(<1..690)
                     /locus_tag="VGR03_13425"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEV1995324.1"
                     /translation="MTLNTDAKAAPEAPRKKPRLALAIATALGVGYIPKAPGTIGSLV
                     GVATAFLSSVFFLRPASVRALFSFHPDYEAVLRNELFPLPGSDIHNAMLAWPIFCALG
                     LLLILGGIGVWSATRAAAYTGTEDPQFVVIDEVAGQHLTLLLPLIPIALPHLTAHMDL
                     SVFAIFFALSLVNWKYLLLGFILFRVFDIWKPWPIRRLEKMPGGWGIMADDWMAGIYA
                     AILMRVALHFGL"
     gene            complement(674..904)
                     /gene="yacG"
                     /locus_tag="VGR03_13430"
     CDS             complement(674..904)
                     /gene="yacG"
                     /locus_tag="VGR03_13430"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEV1995325.1"
                     /translation="MKHKCPICKTETDSAVHADFPFCSERCRERDLGNWASEKYLVSE
                     AVFDEEDIAEGKGNTIVLPLDDDLESPHDIEH"
     gene            complement(1010..2488)
                     /gene="rimO"
                     /locus_tag="VGR03_13435"
     CDS             complement(1010..2488)
                     /gene="rimO"
                     /locus_tag="VGR03_13435"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEV1995326.1"
                     /translation="MTKVGFVSLGCPKNLVDSEVMMGILAREGYELTPRADEAEILVV
                     NTCSFIEAAQRESVEAILEMAEHKKFGAAKKLIVAGCLVERFRAQILEQVPEVDAVVG
                     TGEIERIMEAVEGDLRVLPAQPPAFLYHDLTPRIITTPKHAAYIKIAEGCDHPCTFCI
                     IPQLRGAFRSRRFESVVREAENLARAGAREITLIGQDTTSYGEDLSLRDGLAQLLEKL
                     AQTDGLLWVRFLYAYPNRVTQKLLDTIGAHPRLAKYMDMPLQHASRNVLARMKRGSNG
                     DAFLKLLERIRTTIPGVSLRTSFIVGFPGETEADFEELCDFVKAANLDWMGVFEYSDV
                     DNAGSYALDEKVDAETIGDRRNRLMAIQKKISRENLRAKYLRAKPRVSKNHTFTALIE
                     GPSKDNPLVWEARLEGMAPEIDGKLYLTDIELPGGEVAEAGDVARVEITKTDAYDLIG
                     RVAEILPRPAAQAGVSAAPMPPVPATEKLHRIATGAPLRVLG"
     gene            complement(2609..4852)
                     /locus_tag="VGR03_13440"
     CDS             complement(2609..4852)
                     /locus_tag="VGR03_13440"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEV1995327.1"
                     /translation="MATSIATPTDAALPAERFFRTSLSLLILTSTVTLISTGKLDIVT
                     SFAAPLVVLYKGIRWWHGRPAELSHRAATWFVLAYLAFFPVDALVLSRFSVGNSSNPP
                     LFAALLGAVHFLIFVSIVRFYSAVTDRDALFLSMLSFAGILAAAVLTVDTTFLILFFI
                     FLLFGVSTFVGMELRRGAIGAVSPALHVHTERDRRLNRALSLSALSVASGAIVLGGVL
                     FFFFPRFSAGYLGRASFTSSLMSGFTENVELGQIGEIKKNSAVVMRVQTGKPVAYDRL
                     RWRGIALTTFDGRRWTSSDHKPRELKPKEDGWIYAADSPQKTDSPKPGMIYTIYLEPL
                     ATDAIFVPGKVISLKGNFTGESGNSFGAIRRTYILRDSTDTLLNPFHNYTAVRYSGFS
                     LLPPLNSAKLRAASTEYSPDIRGTYLQLPPLDPRIPELAKQIVKNARTPFDKALVIES
                     YLRNRYTYTLNLTGKPGDDPLAHFLFETRAGHCEYFASAMTIMLRTLGIPAREVNGFL
                     PGEYNDLGGDYIVRASDAHSWVEVYFPGMDWQTFDPTPASPENEAGFLTRLGQYADWM
                     EITWSEWVIGYDFAHQMSLAQNLQRGSRNWSESARDWFEHKQSAGKKWMKSWQLQRGV
                     LGYLLPIALVLFLVALRFNLLAELIRRVRLFFQLRGSKVVRSDPQLASRLYAELLRML
                     ARRGLMRDVSQTPFEFAAAVDSPNLAPAVREFTQLYAHARFGGAPCETMRLRQLLDHI
                     RAALRAR"
     gene            complement(4918..6045)
                     /gene="mutY"
                     /locus_tag="VGR03_13445"
     CDS             complement(4918..6045)
                     /gene="mutY"
                     /locus_tag="VGR03_13445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV1995328.1"
                     /translation="MLNGRELATFRKQLLGWFRQFQRDLPWRRTKDPYRIWLSEIMLQ
                     QTRVAAAIPYYERFLQRFPDVYALAAAPQEEVLRLWSGLGYYSRARNLQKAAQQIVAK
                     HGGKFPARLDAALALPGIGNYTAAAILSIAFGEKQAALDGNVARVLARLGAIRGDLRQ
                     SQLWQELQKTADGLLEPKSPGDWNQAMMELGATRCTPKSPQCLLCPVAQFCEGRKLGI
                     AESLPEKRKKRATVEVILAAAVFTDGKGHTLLLPPPKNTKEKTSADHVPTLVSKMWHF
                     PTVSVTGEPAGNLIAFLRKQIPGVQKRKWHLVPAGKVRHAVTYRAITLLAFLINVKNL
                     PRVSGARQVPLSDICILPVSNLTRKVARAALAAVASARAKP"
     gene            complement(6150..>6337)
                     /locus_tag="VGR03_13450"
     CDS             complement(6150..>6337)
                     /locus_tag="VGR03_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995329.1"
                     /translation="AVLEQLAIAQPLPADPQQSLMADLAASPDLFKIIVTSQPHGSVP
                     ASVWSSSYVVFLDDPVQ"
BASE COUNT         1341 a         1767 c         1939 g         1290 t
ORIGIN      
        1 caacccaaag tgtaacgcca cacgcatcag aattgcagcg tagatgcccg ccatccagtc
       61 atcggccatg attccccagc cgccgggcat tttttccagc cgccggatcg gccacggctt
      121 ccagatgtcg aacacgcgaa aaagtataaa accgagcaaa agatatttcc aattcaccag
      181 gcttaaggcg aagaaaattg cgaagacgga gaggtccatg tgcgcggtca agtgcggcag
      241 cgcgatcgga atcagcggca gcagcagggt caggtgttgt ccagcaactt catcgatgac
      301 cacaaattgg ggatcttcgg tccccgtgta ggctgccgct cgagtagcgc tccatactcc
      361 gatccccccg aggatgagaa gcagacccag cgcacagaat atgggccagg ccagcattgc
      421 attgtgaatg tcgctgccgg gcagagggaa aagttcattt ctcaaaacgg cttcgtagtc
      481 ggggtgaaac gagaataaag ccctaacgga cgcaggccgc aggaaaaaga ccgacgacaa
      541 gaaggcagtc gccactccca cgagcgagcc aatggtgccc ggcgccttgg gaatgtagcc
      601 gactccaaga gcagtggcga tcgccagcgc gagccgcggt ttcttgcgcg gtgcttcggg
      661 cgcggctttg gcgtcagtgt tcaatgtcat gcgggctttc caaatcgtcg tccaaaggaa
      721 ggacgatggt gttccccttg ccttcggcaa tgtcttcttc atcgaagact gcttcggaaa
      781 ccaagtattt ttcgctggcc cagttgccca ggtcccgttc ccggcagcgc tcgctgcaaa
      841 aaggaaagtc cgcgtgaacc gcagagtcag tctccgtttt gcagatgggg catttgtgct
      901 tcatcgcgtg ttttatcttc tctgcgaact ctgtgacctc tgtgttgaaa gattttacac
      961 ggagatcacc gaggccaagg caacgttatc ttcgagagaa gagtatagct cagcccagga
     1021 cgcggagggg tgccccggtg gcgatgcgat gaagcttttc cgtggccgga actggcggca
     1081 tcggcgcagc actaacgcca gcctgtgcgg ctggccgcgg cagaatttct gcgacgcggc
     1141 cgatcaggtc gtaggcgtcc gtcttggtaa tttcaacgcg ggcgacgtcg ccggcttccg
     1201 cgacttctcc accgggcaat tcaatgtccg tgaggtacag cttcccatcg atttccggag
     1261 ccatgccttc gaggcgcgct tcccagacca gaggattgtc cttcgacggg ccttcgatca
     1321 gcgccgtgaa cgtatggttc ttggagaccc gcggctttgc gcgcaaatac ttcgcccgca
     1381 gattctctcg cgagattttc ttttgaatgg ccatcaagcg gttgcggcga tcgccgatcg
     1441 tttccgcatc cactttttca tccagcgcgt agcttcccgc attgtccacg tcggagtact
     1501 cgaacacgcc catccaatcg aggttcgcgg ccttcacgaa atcgcacagc tcctcgaaat
     1561 cggcttcggt ctcgcccggg aagcccacga tgaacgaagt gcgcagtgaa actccaggaa
     1621 tcgtcgttcg aattcgttcc agcaatttca aaaacgcgtc gccgttcgaa ccgcgcttca
     1681 tgcgcgccag cacgttgcgg ctggcgtgct gcagcggcat gtccatgtac ttggcgagcc
     1741 gcggatgcgc cccgatggta tccagcaatt tctgcgtgac gcgattcgga taagcgtaca
     1801 gaaatcgcac ccacagcaaa ccatcggtct gcgcgagttt ttccaggagt tgcgcgagac
     1861 cgtcccgcag gctcaggtct tcgccgtaag aagtcgtgtc ctgcccgatc agcgtgattt
     1921 cccgcgcgcc ggccctcgcc agattctccg cctcgcgcac caccgattcg aatcgccggc
     1981 tgcgaaacgc gccgcgaagc tgcggaatga tgcagaacgt gcagggatga tcgcagcctt
     2041 ccgcgatttt gatgtacgcc gcgtgtttcg gcgtggtgat gatgcgcggc gtcaaatcgt
     2101 gatagagaaa cgctggcggc tgcgcgggaa gcacgcgaag atcgccttcc accgcttcca
     2161 taatgcgttc gatctcgccg gtgcccacga ccgcgtccac ttccggcacc tgctccagga
     2221 tctgtgcgcg aaaccgctcg accaggcagc ccgccacaat cagttttttc gccgcaccaa
     2281 atttcttgtg ctccgccatt tccagaatgg cttccacgga ttctctttgc gccgcctcga
     2341 tgaagctgca tgtgttgacg accagtattt cggcttcatc ggcgcgcggc gtcagttcat
     2401 agccttcgcg cgcaagaata cccatcatca cctcgctgtc cacgaggttc ttcgggcatc
     2461 ccaggctgac gaatccgacc ttagtcatag gcagtaagtt tgcatctttg cgcaattctt
     2521 aattgtaaca tgagcccttc actggcgtgt ttgtaggggc cgccgccgcg gcggtctgcg
     2581 ccccttcttg gcaggacgta atctgtcttc agcgcgcgcg caatgccgcg cgaatgtggt
     2641 caaggagctg gcgcaaacgc atggtctcgc aaggcgcacc gccgaagcgt gcgtgagcgt
     2701 agagttgcgt gaattcccgg acggcgggcg caagattcgg tgaatccacg gcggccgcga
     2761 attcaaacgg tgtctgcgac acatctcgca tcagtccgcg ccgcgccagc attcgcagca
     2821 gttccgcata gagccgcgat gcaagctggg gatcgctgcg aaccaccttg gatccgcgca
     2881 actgaaagaa gagacggacg cggcggatca gttcggcaag aagattgaaa cgcaaagcca
     2941 ccagaaaaag caccagcgcg atcggcaaca aatagcccag cacgccccgc tgcagctgcc
     3001 aggatttcat ccatttcttg cccgcgctct gtttgtgttc gaaccaatcg cgcgcggatt
     3061 cgctccaatt tcgcgagccg cgctgcaggt tctgcgccag cgacatttgg tgagcgaagt
     3121 cgtagccgat tacccattcg ctccaggtga tctccatcca gtcggcgtac tgccccaggc
     3181 gcgtgaggaa gccggcttcg ttctccggac tcgccggcgt cggatcgaat gtttgccagt
     3241 ccatgccggg gaagtagacc tccacccagc tgtgcgcgtc gcttgcgcgg acgatgtaat
     3301 ctccgcccag gtcgttgtat tcgcccggca ggaaaccgtt gacttcgcgt gcaggtatcc
     3361 cgagcgtgcg caacatgatg gtcatcgccg aagcaaaata ttcgcagtgg ccagcgcgag
     3421 tctcgaaaag aaaatgcgcg agcggatcgt ccccaggttt cccggtcaaa ttcaaggtgt
     3481 aggtgtaacg attgcgcaag tagctttcga tgaccagggc cttgtcaaat ggagtgcggg
     3541 cgtttttcac gatctgtttg gcaagttccg ggatgcgcgg gtccagcggg gggagctgca
     3601 ggtacgttcc tctgatatcc ggcgaatatt ccgtcgaagc ggcgcggagt ttcgcggagt
     3661 tcagcggggg caggagcgaa aaccccgaat accggacggc tgtgtagttg tgaaacggat
     3721 ttagcagggt atctgtggaa tcccggagaa tataagtgcg gcgaatcgca ccaaatgagt
     3781 tgccgctttc gccggtgaaa tttcctttaa gagaaatgac cttgccggga acgaaaatcg
     3841 cgtcggtggc cagaggttca agataaatcg tgtagatcat tcctggcttg ggcgaatctg
     3901 ttttctgcgg cgaatccgcg gcgtaaatcc agccatcttc tttgggcttc agctctcgtg
     3961 gcttatgatc cgatgaagtc caccgcctcc cgtcgaaagt cgtaagcgcg atgcccctcc
     4021 agcgcagccg gtcgtaagca accggcttgc cagtttgtac gcgcatgacc accgcggaat
     4081 tctttttgat ctcgccgatt tgaccaagct cgacgttttc cgtgaaaccg gacatgagcg
     4141 aggaagtgaa gctagctcgg ccgaggtacc cggcgctgaa acgcggaaaa aagaaaaaca
     4201 acacgccacc gagaacgatg gctccgctgg ctacgctgag agcggaaaga ctaagtgcgc
     4261 gattcaatct tcgatcgcgc tcggtgtgca cgtggagggc gggcgagacc gcgccgatgg
     4321 ctccgcggcg caactccatt ccaacaaaag tggaaacgcc aaacaagaga aagatgaaaa
     4381 atagaatcag gaaggtggta tcgacggtaa gaacggctgc ggcgagaata cccgcaaagg
     4441 acagcatcga taggaatagc gcgtcgcggt ccgtgacggc gctatagaaa cggacgatgc
     4501 tgacgaaaat cagaaaatgc accgcaccca gaagcgccgc gaataggggt ggattggacg
     4561 agtttccaac agagaaacgc gagagcacca gggcatccac ggggaagaat gccaggtacg
     4621 cgagcacgaa ccaggtggcg gcgcggtgcg aaagttcggc agggcgcccg tgccaccagc
     4681 gaatgccctt gtagagcact acaagaggag ctgcgaagct ggtgacgatg tcgagctttc
     4741 cggtgcttat gagcgtgacg gttgaagtga ggatcagcag tgaaagcgag gtgcggaaga
     4801 aacgctcggc gggcagagcg gcgtcagtcg gagttgcgat ggaggtggcc atggcggact
     4861 ttttatcgta gcacctgggg gttacggcag aaatgaggct gggcgaaaga ataactctca
     4921 gggcttggcc ctagcggaag ctaccgctgc cagagcggcg cgtgcgactt ttctggtgag
     4981 gttggagacg ggcaggatgc agatatcact gagcggaacc tgccttgcgc cggagacgcg
     5041 aggaagattt ttaacattga taaggaaagc taagagggtg atggcgcggt acgttaccgc
     5101 atgacggacc ttgccggccg ggacgagatg ccatttgcgc ttttgcacgc ctggtatctg
     5161 ttttcggagg aacgcgatga gattcccggc tggttcgccg gtcaccgaga ccgttgggaa
     5221 atgccacatc tttgaaacca gcgtcggcac gtgatcggcc gaggttttct cttttgtatt
     5281 tttcggcggt ggcaacagta gagtatggcc ttttccatcg gtgaatactg cggcggccag
     5341 aataacttcc acggtggcgc gcttcttccg tttttcgggc aaggattctg cgatgccaag
     5401 tttgcgccct tcacagaatt gagcgaccgg gcagaggagg cattgtggcg attttggagt
     5461 gcaccgcgtc gcgcccagct ccatcatggc ttgattccag tcgccgggtg atttcggttc
     5521 gagcaagcca tccgctgttt tttgcaactc ttgccaaagc tgtgactggc ggaggtcgcc
     5581 gcgaattgcg ccgaggcgcg cgaggacgcg cgcgacgttg ccgtcgaggg cggcttgttt
     5641 ttcgccgaac gcgatgctca atatcgccgc ggcggtgtag tttccgatgc cgggcaatgc
     5701 gagggccgca tccaagcgcg ccggaaactt tcctccgtgt ttcgcgacga tttgctgtgc
     5761 ggctttttgc aggttgcgcg cgcggctgta atacccgagc cccgaccaga ggcgcagaac
     5821 ttcttcctgc ggcgcagcgg cgagcgcgta aacgtccggg aagcgttgca gaaatcgttc
     5881 gtaataagga atcgcggcgg caacgcgggt ttgctgcagc atgatctccg agagccagat
     5941 gcgataggga tctttggtgc ggcgccacgg caggtcacgc tggaactggc ggaaccatcc
     6001 gagcagttgt ttgcgaaatg tggcgagctc gcggccgttc agcatagtgc tggcacattc
     6061 tcgcacagct gagtgggaca gacattcttg tctgtccggt tcccgaaagt ttgttggacg
     6121 aagggggcag gcaggaatgc ccgtcccacc tactgcacag ggtcgtcgag gaagacgacg
     6181 taagaggaac tccagacgga agcgggaacg gagccgtggg gctggctggt cacgatgatt
     6241 ttgaagagat cgggcgaggc cgcgagatcg gccatgagcg attgctgggg atcggctggc
     6301 aatggttgcg cgattgccag ttgctcgagg actgcga
//
LOCUS       DASYVO010000403         5109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_277417, whole genome shotgun sequence.
ACCESSION   DASYVO010000403 DASYVO010000000
VERSION     DASYVO010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277417"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="VGR03_13455"
     CDS             <1..1147
                     /locus_tag="VGR03_13455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV1995330.1"
                     /translation="AGGWFFLMACEMFVLGKRDFRLPGLGSFLQTAASSGNTRAILWG
                     VAAMIAVIVLLDQLVWRPIIVWADKFKFEQVESSGAAQSTLLHLIGRASIVLRLYRFI
                     LQPVFHWLTLTFTLGARRAAETFSAPKQNRLRRWIGYLLAAGVLVGLGFAVFHAAREL
                     SGLHREDYDELLESAALTFLRVNTALILGALWTVPVGVAIASNPRVARIALPLVQMAA
                     SIPATALFPIILLFLLRLRGGLEIAAMLLMLLGTQWYILFNVIAGAMAIPTDLKEAAQ
                     IYRFGSWDRWRHLILPGIFPYLVTGMVTASGGAWNASIVAEYFHFQGRIVSTAGLGST
                     ISSASDSGRFDVLLASTLIMATVVVLINRLLWRRLYRLASSRFKLEA"
     gene            1191..1643
                     /locus_tag="VGR03_13460"
     CDS             1191..1643
                     /locus_tag="VGR03_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995331.1"
                     /translation="MPESKFTSGASAWSDEDFLRAFEDLSFPADLFHHREHIHVAWLY
                     LKSTDASRAAERMSEGIRRFANHRGATQKYHHTLTLAWMRLVAAALVETPEGYTFEQF
                     ITAHPQLKDTNLLAQYYSKELLQTAAAREAWVEPDLQPLPDLRIYRCC"
     gene            complement(1788..1961)
                     /locus_tag="VGR03_13465"
     CDS             complement(1788..1961)
                     /locus_tag="VGR03_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995332.1"
                     /translation="MVEQKQLTKGKAALLLMLSAVGAIVSATQLHGWHIYLGFALVGI
                     GVFIGFIRWNRKY"
     gene            complement(2024..2587)
                     /locus_tag="VGR03_13470"
     CDS             complement(2024..2587)
                     /locus_tag="VGR03_13470"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV1995333.1"
                     /translation="MAESSVWPEGIKPAALKFIQVTCGMGAKGSSFMAEVNETVVAGL
                     AGYYKFVAAQLHKWVDRLSNEQFWRKPYPYGNSVGHLVLHMTGNLNYYIGARVAETGY
                     VRDRDREFTDKQPPPKEEALAAFDRTIAMVAATIRKQSAEDWGKAYMAEREPEAADRF
                     QIFVRCAGHAYHHVGQIVYLSRELART"
     gene            complement(2699..3040)
                     /locus_tag="VGR03_13475"
     CDS             complement(2699..3040)
                     /locus_tag="VGR03_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995334.1"
                     /translation="MSEISYADALNTFGRWESQSKKLAVLGSNSGCVISLRNAYVTLC
                     LDDMLQLSFSEDGILRFFLRGATFSPADPKDFPADSGIWSADLEAGVQIRFVNIEMQC
                     FIFPARGNRSD"
     gene            3566..4678
                     /locus_tag="VGR03_13480"
     CDS             3566..4678
                     /locus_tag="VGR03_13480"
                     /inference="COORDINATES: protein motif:HMM:NF024151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995335.1"
                     /translation="MRRRRAIRRINNVFLIAVLSLATAFFITRPTGGAGFSAPPPGLH
                     PAAAAPSATLVSPSTDANGYELEPGPYTVSEVEDLVLHDAKRGKDLHVRIFYPNDSGK
                     YPVIVFSHGAGGSQTCCDGLTRHWATYGYVTIQPTHDDSAVRRRNQGEENVRFTQAVR
                     DALKKPALWESRPLDISYVLDALHSIESRIPGPSGKMDADRIGVGGHSMGSYTTEAIA
                     GALIDLPGHSARNFADPRVKAVLCLSPQGPGQFGLNDRSFDRISLPYMGMTGSLDSLG
                     PVANPAWHKTPFDRSQPGDKYHLFIEGANHMSFITPRTLLPGRNEKGGTIFDYTNAAS
                     LAFWDAYLKGDARAKSYLQSGALEKSSHGAVKLFLR"
     gene            4863..>5109
                     /locus_tag="VGR03_13485"
     CDS             4863..>5109
                     /locus_tag="VGR03_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995336.1"
                     /translation="MARYSSFLGRRVEVQYRAGDILLPASGTFVADSGRSIFLEQNFE
                     QRGQHKHFRWEIPYQYLVRIEEKPDSGASANGVSAPAT"
BASE COUNT          919 a         1741 c         1238 g         1211 t
ORIGIN      
        1 cgccggcggt tggttttttc tcatggcttg cgagatgttc gtcctcggca agcgcgactt
       61 tcgccttccc ggccttggct ccttccttca gaccgccgcg agcagcggca acactcgcgc
      121 gattctgtgg ggcgtcgctg ccatgatcgc cgtcatcgtt ctcctcgatc agctcgtttg
      181 gcggcccatc atcgtttggg cagacaagtt caagttcgag caggtcgaaa gttcaggcgc
      241 tgcgcagtcc actttgctcc atctcatcgg ccgcgcttcc atcgtcctcc gcttgtatcg
      301 cttcatcctt cagcccgtct tccactggct cactcttacc ttcactctgg gtgctcgccg
      361 cgccgcggaa actttttctg cgccgaagca gaaccgcctg cgccgctgga tcggctatct
      421 gctcgccgca ggtgttctgg ttggtttggg ctttgccgtt ttccacgcag cccgcgaatt
      481 atccggtctt caccgcgagg actatgacga gctcctggaa agcgccgcgc tcacttttct
      541 tcgcgttaac accgcgctca tcctaggcgc gctgtggacc gttcccgtcg gcgtcgccat
      601 cgcttccaat cctcgcgttg cccgcatcgc gctgccgctg gtgcagatgg ccgcctccat
      661 tcccgccacc gccctctttc cgattattct tctttttctg cttcgactcc gcggcggcct
      721 cgagattgcc gccatgctcc tgatgctcct cggcacgcag tggtacatcc ttttcaacgt
      781 cattgccggg gccatggcca tccccaccga cctcaaggaa gccgcgcaaa tttatcgctt
      841 cggctcttgg gaccgctggc gccacctcat cctgcccggc attttcccgt atctcgtcac
      901 cggcatggtc acggcctccg gcggtgcctg gaacgccagc atcgtcgcgg aatattttca
      961 tttccagggc agaatcgttt cgactgccgg actcggcagc accatcagca gcgctagcga
     1021 ctccggccgc ttcgatgtcc tgctcgcttc gacgctcatc atggccaccg tcgtcgtgct
     1081 catcaatcgg ctgctctggc ggcgcctata tcgcctcgcc tcctctcgct tcaagctgga
     1141 ggcgtagtac aatccagtga gcgcgaatct cttagttctt ccaaactcct atgcctgaat
     1201 ccaaattcac ctctggcgct tccgcgtgga gcgacgagga ctttcttcgc gctttcgagg
     1261 atctatcgtt tcccgccgat ctctttcacc accgggaaca catccacgtt gcctggctct
     1321 acctgaaatc caccgacgca tctcgcgccg cggaacgtat gtcggagggt attcgccggt
     1381 tcgcgaatca ccgcggcgcg acgcagaaat accatcacac gcttacattg gcttggatgc
     1441 ggctggttgc cgccgccttg gtggaaacac cggagggata cacgtttgag cagttcatca
     1501 ccgcccatcc gcaactgaag gatacaaacc ttctcgccca atattattcg aaagaactcc
     1561 tgcagactgc cgctgcccgc gaagcctggg tcgaaccgga cctccagccg ctcccggacc
     1621 taaggattta ccgctgctgc tgatttcttc tcttccgctt tcggttcagg cgtctttggt
     1681 tcctgtggct cttcgcccca gatgtattgc gcgaaccatt tttcgttttc ttccatcacc
     1741 gcgcgttgct gtttcggttt cgtaattcca tgcccggaga cgtgaactca atacttgcgg
     1801 ttccagcgga taaatccgat gaaaacacca attcccacga gggcaaatcc caggtagatg
     1861 tgccagccat gcagctgcgt cgccgacacg atcgcgccta cggcggatag catcagcaac
     1921 agggcagcct tgcccttcgt tagttgcttc tgttcgacca ctcgcgtttc agttctcgcc
     1981 gcgacgatgc ccactctgca ccgcgccggc gtttttcccg gccttaagtt ctcgccaact
     2041 ctcggctcag gtacacaatc tgccctacat gatgatacgc atgccccgca caacgcacga
     2101 aaatttggaa gcgatccgcg gcctccggct cccgctcggc catgtacgcc tttccccagt
     2161 cctccgcact ctgttttcga atcgtcgccg ccaccatggc aatcgtgcgg tcaaacgctg
     2221 ccagcgcttc ttcctttggc ggtggctgct tgtccgtaaa ctcgcggtcg cgatcacgca
     2281 cgtaccccgt ctccgcgacg cgtgctccga tgtagtagtt cagattgccg gtcatgtgca
     2341 gcaccaggtg tccaacgctg tttccataag gatacggctt gcgccagaac tgttcgttcg
     2401 aaagccggtc cacccacttg tgcaactgcg cggcaacaaa tttgtaatac cctgccagcc
     2461 ccgcaaccac cgtttcgttc acttccgcca tgaaagaaga cccctttgca cccattccac
     2521 aggttacctg gatgaacttc agcgccgctg gttttattcc ctctggccaa acgcttgatt
     2581 cggccattct cctgctgcac gccttcccat ttctccatcg tttccaatcg aatcccggaa
     2641 cgccgacaat agcctctctc tgcaaaatgt caaggaattc gctctcgcac cgctagcttc
     2701 aatcggaacg attgccccgc gcaggaaaaa tgaagcattg catctctatg ttcacaaacc
     2761 ggatctggac acctgcttca agatccgcag accaaattcc ggagtctgcc gggaaatctt
     2821 tgggatctgc cggcgaaaaa gttgcgccgc ggagaaagaa gcgcaaaatc ccgtcctccg
     2881 aaaatgagag ttgcaacatg tcgtccaggc aaagagtcac gtaggcattc ctgagggaaa
     2941 tcacacagcc ggagttggag cctagcactg ccagcttctt gctctgactc tcccatctgc
     3001 caaaagtgtt cagcgcatcc gcgtaggaaa tttcactcat tcgtacacct cttgctttgc
     3061 gaatccccag cgcgttggct attctaaccg tgtaagccgc ccgtgtcccg ccgcttttca
     3121 ggagctctcc gcgcccgcag ttccggctct tggcattcga tcgtgcaacg acccgcaatc
     3181 cgcgccgcgt ttctagttgt tccgtcggaa ccattcgctc gaacagccgc gcggagtagc
     3241 ttcttctttg ctacgcttac agttgctctc tcgccaaaca gtcttattca gcctctgatc
     3301 tggcgaggat tccagcccgc aaccggagaa tcagccgtaa cgtttgccca agccttcggg
     3361 agatttcccc gttggtctgc ggagtacaac ttcaaggatg gttttcagtg caattcgagg
     3421 tagagcgatc cgaaacctgt tccgcaactt attgatccgg actgggtttg taaccctgtc
     3481 tgctgaaaat tcagcgaatt tcaaacacgc tatgtccccc atgacacatg cccggagatt
     3541 ttttccttct ctcccgcgag actatatgcg ccgccgccgc gccattcgcc ggatcaacaa
     3601 tgttttcctg atcgctgttc tgagcttggc cactgctttt ttcatcaccc gacccacggg
     3661 cggcgccggt ttctccgcgc cgcctcccgg gctccatcca gcggccgccg ctccgtccgc
     3721 cactctggtt tccccctcga ctgacgcgaa cggctacgag ttggagccag ggccatatac
     3781 cgtctccgaa gtcgaagacc tcgttctcca cgacgcgaag cgcggcaaag acctgcacgt
     3841 gcgaattttt tatcccaacg attcggggaa gtacccggtc atcgtctttt cgcacggcgc
     3901 cggcggctcg cagacctgtt gcgacggcct cacgcgccac tgggccacct acggctacgt
     3961 caccatccag cccacccacg atgactccgc cgtccggcgc cgcaaccagg gcgaggaaaa
     4021 tgtccgcttt acgcaagccg tccgcgacgc ccttaaaaag cctgcacttt gggaaagccg
     4081 cccgctggac atctcttacg ttctcgatgc gctgcactcg atcgagagcc gcatccccgg
     4141 cccctccgga aagatggacg cggatcgcat cggcgtcggc ggccattcca tgggttccta
     4201 caccacggaa gccatcgccg gcgcactcat cgatcttccc ggccattccg ccagaaattt
     4261 tgccgatcct cgcgtgaaag ccgttctttg cctttcgccg caagggcccg gccaatttgg
     4321 tttgaacgat cgctccttcg atcgaatatc tctgccctac atgggcatga ccggctcgct
     4381 cgacagcctc ggccccgttg ccaatcccgc ctggcataaa actccttttg accgcagcca
     4441 gcccggcgac aagtaccatc tcttcatcga aggcgcgaac cacatgtcct tcatcacccc
     4501 gcgcacgctt ctgccaggcc gcaatgagaa aggcggaacc atcttcgatt acaccaatgc
     4561 cgcgtctctt gctttttggg acgcctacct gaagggcgac gcgcgcgcga agtcctacct
     4621 ccaatccggc gcgctggaaa aatccagcca cggcgccgtc aaacttttcc tccgctgatc
     4681 tcttcctggc agccgatctt ttctccaatc ctccgtgctc tccgcggcct tcacattgaa
     4741 tctttatctt gcctgctttt tttctacagt cgagcgcccc tcccgttttt ccacagttct
     4801 cggtaggggg tccttccgta ctattgaccc tgttactacc ccccacggac actgggaaca
     4861 ggatggctcg ctactcgtct tttctcgggc gtcgcgtgga ggtgcaatat cgcgccggcg
     4921 atattcttct gcccgcttcc ggcactttcg tcgccgattc cggccgttcc attttcctcg
     4981 aacagaattt cgagcagcgc ggacagcaca agcacttccg ttgggaaatc ccttatcaat
     5041 atctggtgcg catcgaagaa aaacccgact ccggtgcttc cgcgaacggc gtctctgctc
     5101 ccgccaccc
//
LOCUS       DASYVO010000404         4276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_277460, whole genome shotgun sequence.
ACCESSION   DASYVO010000404 DASYVO010000000
VERSION     DASYVO010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277460"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..2484
                     /locus_tag="VGR03_13490"
     CDS             97..2484
                     /locus_tag="VGR03_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1995337.1"
                     /translation="MSLPILQGLAKHKDPVCGMMVAPEKAAGKVEHAGKTYYFCSKRC
                     AERFSHEPEKFLAAPGTGGMQHGPAPEKHSAMDQTPASSEAAIASTKAIRYTCPMHPE
                     IVQIGPGVCPKCGMALEPMDVFADVEADPEYDSMRLRFWVSAALSLPVLLLSMLGESL
                     GLHLTPTVRNGIEFLLATPVVLWGGWPFFERFWSSLVNRSPNMFTLIGLGTGAAYLDS
                     VAATFFPQIFPASIRGMDGAAPVYFEAAAVITTLVLLGQVLELRARQRTSGAIRALLN
                     LAPPQAHVLDSTGGEKDVTLDQVKHGDRLRVRPGERVPVDGTIREGASAVDESMVTGE
                     PMPVEKSTGDNVTGGTLNTSGSFIMEAERVGSETMLAQIVKLVSEAQRSRAPLQRLAD
                     RVAAYFVPAVVLTAVLTFFGWIIFGPEPRFAHALVSAVSVLIIACPCALGLATPISIM
                     VAVGRGAHAGVLIRNAEALETLAKVDTLVVDKTGTLTEGKPQVGNIVVFEESQVSRTQ
                     LLRLVASVERASEHPLGQAIVRAAEDEKLLTQSVEEFQATPGGGAEGKVAGKHVLAGT
                     AKFLKERGVQLPVGKSMGVLTVGAASVTAVYAAIDGKAAGAFFLTDKVKSSSAGAIQQ
                     LKAEGIRVVMLTGDRAESAKSVATQLQIEETKAEVRPEGKSEIVKNLKNEGRVVAMAG
                     DGINDAPALAAADVGIAMGTGTDVAIENAGITLLKGDLRGIVRARKLSRATIRNIKQN
                     LAFAFLYNVIGIPIAAGVFYPIFGWLLSPMLASAAMSFSSVSVIANALRLRKTAL"
     gene            complement(2502..3647)
                     /locus_tag="VGR03_13495"
     CDS             complement(2502..3647)
                     /locus_tag="VGR03_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995338.1"
                     /translation="MHISRIRPTFGVLLAGLTMGFVLSVHSLAGAGTGPDVPKFKVDP
                     SWPKELPNNWIMGQVGGMAVDRHDHIWVLQRPGLDTVDELGAAQTPPWSQCCFAAPPV
                     LEFDTQGNLLQFWGGPGAGFDWPKREHGIYVDRDDNVWIGGADPSDRHLLKFKNDGHF
                     LMQIGHPSTDPPNSLRTDILGRPAGIEVDSEAHELYIADGYMNKRVIVYDSDKGTFKR
                     LWGAYGNPPNDADPGPYNPAAPPDQQFRNPVHCVHISRDGLVYVCDRVNDRMQVFTKQ
                     GKFVKEFFVRPQTLAIGSVWQFAFSVDENQNYLLVADGENNVIWTVRRDDGTVVGQTG
                     HNGRNAGQFHWVHQIVSDSQGNLYTGEVDTGKRIQKFVLLHEDAIRH"
     gene            complement(3795..>4276)
                     /locus_tag="VGR03_13500"
     CDS             complement(3795..>4276)
                     /locus_tag="VGR03_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995339.1"
                     /translation="SPDGRFVVFARTENKVSHLYIASTDGSGEPRRVIETPGGVPRWS
                     SDGKWISFSPNRGFSSGVFIVHPDGTGLRRLTENGGWAVWWPDSEQIGFQVIGADGNE
                     EIQVFSLKSGETRTLSKLHFVGTNFPFDVSRDGKWLVTTNYQHISDEIWLLEPVGKK"
BASE COUNT          913 a         1259 c         1245 g          859 t
ORIGIN      
        1 tgtacaacga actgctcgaa ctaatctaca cgcacttgcg atagcaccgc cgaggcaaga
       61 ggagatcacc caatgaattc gcaggatgca ggcggaatgt cacttccgat tttgcagggg
      121 ctggccaagc acaaggatcc cgtttgcgga atgatggttg ctccggagaa agcggctggc
      181 aaagttgagc acgcgggaaa gacatactac ttctgttcga agcgttgtgc cgaacggttt
      241 tcccacgagc cggagaagtt tctggccgct cccgggaccg gaggaatgca acacggcccc
      301 gcgcctgaaa agcattccgc gatggatcaa acgccggcct cttcggaggc tgcaatcgca
      361 agcacaaaag cgatccgcta tacgtgccca atgcatccgg agattgtcca gatcgggccg
      421 ggggtttgcc cgaaatgcgg gatggcgctt gagccgatgg acgttttcgc ggacgtggag
      481 gccgatcccg agtacgactc gatgcgcctg cgcttctggg tcagcgcggc gttatcgctt
      541 ccggtcctgc tgctttccat gctcggcgaa tcgctcggcc tgcatctgac gccaaccgtt
      601 cgcaacggca tcgaatttct tcttgccacg ccggtggttc tctggggtgg ctggcccttt
      661 ttcgaacgct tctggtcgtc gctcgtcaac cgcagcccga atatgttcac gctgattggc
      721 ctgggcacgg gcgctgcgta tctcgacagc gtcgcggcca cttttttccc gcagatattc
      781 ccggcatcca ttcggggcat ggatggcgcc gcgcccgttt atttcgaagc ggccgcggtg
      841 atcactacgc tggttctcct ggggcaagtc ttggaattgc gcgccaggca gcgaaccagc
      901 ggagcgattc gcgcgttatt gaatctcgcg ccgccgcagg cgcacgtgct ggactccact
      961 ggaggggaaa aggacgtcac gctcgatcag gtgaagcacg gcgaccggct ccgtgtccgt
     1021 cccggtgagc gcgttcccgt cgacggcacg atacgtgaag gcgcgagcgc cgtcgatgaa
     1081 tcgatggtga ccggcgaacc catgccggtc gagaagagca cgggcgacaa cgtcaccggt
     1141 ggcacgctga acaccagcgg cagtttcatc atggaagcgg agcgcgtcgg cagcgagacc
     1201 atgctcgcgc agattgtgaa actcgtcagt gaagcccagc gcagccgcgc gcccttgcag
     1261 cgcctcgccg accgcgtcgc agcctatttt gttccggcgg tggtcctgac agccgtactc
     1321 acttttttcg gctggatcat cttcggtccg gagcctcgct tcgctcacgc gctcgtcagc
     1381 gcggtttccg tgctcatcat cgcatgtcct tgcgccctcg gcctcgcgac gcccatctcc
     1441 atcatggttg ctgttggtcg cggcgcgcac gccggggtcc tcatccgcaa tgccgaagcc
     1501 cttgagacac tcgcgaaagt tgacaccctc gtcgtggaca agaccggcac tctgacggaa
     1561 ggtaagcccc aagtggggaa tatcgtggtc tttgaggaga gtcaggtttc gaggacgcaa
     1621 ttgcttcgat tggtagccag cgttgagcga gccagcgaac atccactggg acaagcgatt
     1681 gtgcgcgcag cggaggacga gaaactattg actcaatccg tcgaggaatt ccaggcgaca
     1741 ccgggagggg gtgcggaagg caaagtcgca ggcaaacacg tattggccgg aaccgcgaag
     1801 tttttgaagg aacgcggtgt tcagcttcca gtaggaaaaa gcatgggggt tctcaccgtt
     1861 ggagcagcat cggtgaccgc ggtttacgca gcgatcgacg gcaaagctgc aggtgctttt
     1921 ttccttaccg acaaggtaaa gagttcatca gccggagcga tccagcaact gaaggctgag
     1981 ggtattcgag tggtgatgct cactggagat cgggcggaat ctgcaaaatc cgtagccact
     2041 caactgcaaa tcgaagaaac caaggcggaa gtcaggcctg aggggaaaag cgagatcgta
     2101 aagaatctga agaatgaagg ccgcgtcgta gccatggccg gggacggaat caacgacgca
     2161 ccggcgctgg ctgctgcgga cgttggcatc gccatgggca cgggaaccga cgtcgccatc
     2221 gaaaacgccg gcatcacact cctaaaaggc gacctgcggg gaatcgtgcg ggcgcgaaaa
     2281 ctgagccgcg ccacgattcg caacatcaaa cagaaccttg cattcgcctt tctttacaac
     2341 gtgattggaa ttcctatcgc cgcgggggtt ttctatccca ttttcggctg gctactcagc
     2401 cccatgttgg ccagcgcggc gatgagcttc agttccgtct ccgtgattgc caacgctctt
     2461 cgcctgcgaa aaacggcgct ctaacgacat taatcgtcga ttcagtggcg aattgcatcc
     2521 tcgtgcagca agacaaattt ctgaattcgt ttgccggtgt cgacctcgcc cgtgtacaag
     2581 ttgccttgcg agtcggacac tatctgatgc acccaatgaa actggccggc gttgcggccg
     2641 ttgtgacccg tttggcccac gaccgttcca tcatcgcggc gcaccgtcca gatcacattg
     2701 ttctcgccgt cggcgacaag aagataattc tgattctcgt ccactgaaaa cgcgaattgc
     2761 cagacggatc ctattgccag cgtctgtggc cgcacaaaaa actccttcac aaacttgccc
     2821 tgcttggtga agacctgcat gcggtcattc acccgatcgc aaacgtagac caagccatcc
     2881 cgcgagatgt ggacgcaatg aacgggattg cggaactgct ggtctggcgg tgccgcggga
     2941 ttgtaaggac cgggatccgc atcatttggc ggatttccgt acgcgcccca caagcgtttg
     3001 aatgtgcctt tgtccgaatc gtagacgatg acccgcttgt tcatgtaacc gtcggcgatg
     3061 tacaactcat gagcttcgga gtccacttcg atgccggcgg gccgcccgag aatgtcggtg
     3121 cgcaagctgt ttggcggatc ggtggaaggg tggccgatct gcatgaggaa atgcccgtca
     3181 tttttgaact tgagcagatg acgatcggaa gggtcggcgc ccccgatcca gacgttatcg
     3241 tccctgtcca cgtaaatccc gtgctctctt tttggccaat cgaatcccgc gcctggacct
     3301 ccccaaaact gcagcaggtt tccctgcgta tcgaattcga ggaccggagg cgcggcgaag
     3361 cagcactgcg accacggagg cgtctgcgcg gcacccaatt cgtcgaccgt gtccaagccg
     3421 gggcgttgca atacccaaat gtggtcgtgg cgatccacgg ccatgcctcc cacttggccc
     3481 atgatccagt tatttggcaa ttcctttggc caggacggat ccaccttaaa ctttggcacg
     3541 tctggaccag tccccgcccc agcgagggag tggacggaca aaacaaatcc catcgtcaag
     3601 ccagccagaa ggacgccaaa cgtcggccga attcgagaaa tatgcatggg acactccatt
     3661 ggacaaaact caggacgagc aagcagtgcg cggagtatag gtccgcggta ccgggctgtc
     3721 aacgaaacga agcctgtgtg ccaccgcaag atgaggacgt cccgctactg cagagccgaa
     3781 gtgccaaagc gaaatcactt cttgccaaca ggttccagca gccagatctc atccgaaata
     3841 tgctggtagt tcgtggtgac gagccacttt ccatcccgcg atacgtcgaa tggaaaattc
     3901 gtccccacga agtgcagctt cgagagcgtt cgcgtttccc ccgacttcag gctgaacact
     3961 tggatctctt cgttcccatc cgccccaatg acctgaaagc cgatctgctc cgaatcgggc
     4021 caccacaccg cccatccgcc attttccgtg aggcgccgca aaccggttcc atccggatgc
     4081 acaatgaaaa cgccgctgga gaatcctcgg ttggggctga aactgatcca ctttccgtcg
     4141 gaagaccagc gaggtacacc tccgggagtt tcaataacac gcctaggctc gccgcttccg
     4201 tccgtggacg caatgtagag atgcgaaact ttattctctg tgcgagcgaa taccacgaag
     4261 cgtccgtcgg gtgaaa
//
LOCUS       DASYVO010000405         3008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_39815, whole genome shotgun sequence.
ACCESSION   DASYVO010000405 DASYVO010000000
VERSION     DASYVO010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39815"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..904
                     /locus_tag="VGR03_13505"
     CDS             332..904
                     /locus_tag="VGR03_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995340.1"
                     /translation="MKSSNSPVSDKIVAPVSTRCLFSFSDGRQCRTPRQPAVAGQFGH
                     PHFCFFHARKEAQARAADEAGREVSGFLSGSYLTACDLSSALARLFAAVAQGQIKPRT
                     AATLAYLGQTLVQTIQLAQDEYINAFSTDGWRRAVRSSFPGQPHEDDDLDQDEAENPE
                     QPTGALLSSPSERAMLKSLESTLAKVYQNK"
     gene            1057..2325
                     /locus_tag="VGR03_13510"
     CDS             1057..2325
                     /locus_tag="VGR03_13510"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV1995341.1"
                     /translation="MRFRCFLILLVVLAICALPAAAQAPEQPVAPQITAIRAGKVVDP
                     QTGQVSANQIILVQGRIIREIGPGIAIPRGAKVVDLSKATVLPGLFDAHTHLCMTVKL
                     KRDAANYYFTTLLDTTAYRAIEGVANSRDMLASGFTTVRDVGNAGNYADTDLRRAIEA
                     GLVPGPTIINSGIIIAPYGGQFHLQPEKKDLATPEYLFADTRDEMRKAIRQNIHFGAR
                     LIKIVVDDQDYIYSVDDIRFMIAEAHASGLKLAAHCWTHPGAHNAAEAGVDSIEHGFR
                     MTDEDLQLAKKNNVTLVGTDFTEKMTRALGNPWEHEVWVDRLRRAYTIGVNMAFGTDV
                     DIALAGETRGTLTIDYISTWVEAGVPPADILRAMTINAARLLGVDKERGALKPGLAAD
                     IIAVPENPLDNINTLRKVSFVMKDGVIIKQ"
     gene            complement(2367..>3008)
                     /locus_tag="VGR03_13515"
     CDS             complement(2367..>3008)
                     /locus_tag="VGR03_13515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1995342.1"
                     /translation="LKNQFHEVKKEHRIVEHIAAKGDVLQPEYHKATPECPHPERWRM
                     YDSMSAEVEVLDFLKSLVRTLKPELVVETGTFTGLSTLRIAEGLRENGFGRVVTCEYD
                     KKVFDAAQKRFAESGLEKWIEARNESSLETKIEGRIDLLFCDSDAPLREREVRRFLPQ
                     ISPHGIIVMHDASSSLETVREAALRMEQEGLLSVLLLPTPRGLALAQKREGRK"
BASE COUNT          589 a         1082 c          735 g          602 t
ORIGIN      
        1 tcttgagttt cctgatcccc ctgctccccc cgcggcggga tccctgcccc ctccccgcca
       61 gccttcctcc atcgcgcctc agcattctct gtgccctctg tgtcctctgt ggtgaacctc
      121 agttcacgag agttcgctcc cccctcgccc ccaacctctt ccccttcaac gacttacaaa
      181 aaatacttga caatgactgt acctttgcgg taacatatct tgtgagttcg acgctacagg
      241 tcgccttcct gcctccgcaa gcttgccctg agcgtaagcg tagggttacc tcactgcttc
      301 catacttcag tacttcatcc taggagcctc catgaaatcc tctaactccc ctgtttcaga
      361 caagatagtc gcgcccgttt caacccgatg cctcttctcc ttctccgacg gccgccagtg
      421 ccgcacgcct cgccagcccg ccgtggcggg ccagttcggc catccccatt tctgcttctt
      481 ccatgcgcgc aaggaagcgc aagcccgcgc cgccgacgaa gccggccgcg aggtctccgg
      541 gttcctatcc ggaagctacc tcaccgcttg cgatctcagc tccgctctgg cacgcctttt
      601 cgccgccgtc gcccagggcc aaatcaagcc caggaccgcc gccaccctcg cctatctcgg
      661 ccaaaccctg gtacaaacca tccaactcgc ccaggacgag tacatcaacg ccttcagcac
      721 cgacggctgg cgccgagccg tccgctcctc cttccctggt caaccacacg aggacgacga
      781 cctggaccag gacgaagccg aaaacccgga gcaacctacc ggagccctgt tgagttcccc
      841 aagcgaacgc gccatgctaa agtctttgga atcaacactt gcaaaagtgt atcaaaacaa
      901 atgacttcaa ctccctttag aatgaacact tacaaaaaac aggggggagg gggagtatct
      961 cttctgtcaa cagttctagt accgcgcttc cctgcctcct tacttccctg cttccacact
     1021 ctgtgctaca acctccttcc tcggaggact aacctgatgc gattccgctg cttcttgatc
     1081 ctgctcgtcg tcctggcgat ctgcgccctg cccgccgctg cgcaggcgcc cgaacaaccg
     1141 gttgcgccgc agatcaccgc catccgcgcc ggcaaggtcg ttgacccgca aacgggccag
     1201 gtctccgcca accagatcat tctggtccaa gggaggatca tccgggagat cggcccaggc
     1261 attgccattc cacgcggcgc gaaagtcgtg gacctctcga aggccacggt cctcccgggc
     1321 ctgttcgatg cgcacactca cctctgcatg accgtcaagc tcaagcgcga cgccgccaac
     1381 tactacttca ccacgctcct tgacaccacc gcctaccgcg ccatcgaagg cgtcgccaac
     1441 tcccgcgaca tgcttgcctc cggcttcacc accgtgcgcg acgtcggcaa cgccggaaat
     1501 tacgccgaca ccgatctccg ccgcgccatc gaagccggcc tggttcccgg ccccaccatc
     1561 atcaactccg gcatcatcat tgctccctat ggcggccagt ttcatctcca gcccgaaaag
     1621 aaagacctgg ccacgcccga atatcttttc gccgacaccc gcgacgaaat gcgcaaggcc
     1681 atccgccaga acatccactt cggcgcaagg ctcatcaaga tcgtcgtgga cgatcaggac
     1741 tacatctatt ccgtggacga catccgcttc atgatcgccg aggcccacgc ctccggcctc
     1801 aagcttgcag cccattgctg gacgcatccc ggcgcgcaca atgccgccga agccggcgtg
     1861 gactccatcg agcatggctt tcgcatgacg gacgaggatt tgcagctcgc gaagaagaac
     1921 aatgtcaccc tggtcggcac ggatttcacg gagaagatga cccgggcact tgggaatccc
     1981 tgggagcacg aagtttgggt ggaccgcctc cgccgcgcct acacaatcgg cgtcaacatg
     2041 gccttcggca cggacgttga catcgcgctc gccggagaaa cccgcggcac cctcacgatc
     2101 gattacatca gcacgtgggt cgaggctggc gttcctccgg cggatattct ccgcgcgatg
     2161 accatcaacg cggcacggct cctcggcgtg gacaaggaac gcggtgcatt aaagcccggc
     2221 cttgccgcag acatcatcgc cgttccggaa aatcctctcg acaacatcaa cacactgcgc
     2281 aaagtctctt tcgtcatgaa ggacggagtc atcatcaaac aatgattttc agatctctgc
     2341 ggggtggaca aacaagaacg aaggccttac ttgcgccctt cgcgtttctg tgccagcgcc
     2401 aggccccgcg gagtcggcag cagcagcacg gacaaaagcc cttcctgctc catgcgcaac
     2461 gcggcttccc ttacggtttc gagcgacgag ctggcatcgt gcattacgat gatgccgtgc
     2521 gggctgatct gcggcagaaa gcgccgcacc tcccgctcgc gcagcggggc gtcgctgtcg
     2581 caaaaaagca gatcgatccg cccctcgatc ttcgtttcca aactcgattc gttccgcgct
     2641 tcgatccact tctccagccc cgattccgcg aaacgtttct gcgccgcgtc gaaaactttc
     2701 ttgtcgtact cgcacgtcac cacgcgcccg aacccgtttt cccgcaggcc ttcggcgatc
     2761 cgcagcgtgc tcaatcccgt gaacgtgccc gtttcgacca ccagctcggg cttgagcgtt
     2821 ctgaccaggc tcttcaagaa gtcgagcacc tcgacttccg ccgacatcga atcgtacatg
     2881 cgccaccgct ctgggtgcgg gcactccggc gtcgccttgt ggtactccgg ttgcagcacg
     2941 tcgcccttcg ccgcgatatg ctccacaatt cgatgttctt tttttacttc gtggaattgg
     3001 tttttaag
//
LOCUS       DASYVO010000406         6399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_125331, whole genome shotgun sequence.
ACCESSION   DASYVO010000406 DASYVO010000000
VERSION     DASYVO010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125331"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1543)
                     /gene="ilvD"
                     /locus_tag="VGR03_13520"
     CDS             complement(<1..1543)
                     /gene="ilvD"
                     /locus_tag="VGR03_13520"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV1995343.1"
                     /translation="MSDSLLKPRSHTLTDGAHRAPARAMLRASGLRDEDFKKPLVGVA
                     NTWIEIGPCNYHLRELAQHVKEGIRAAGGTPLEFNTVSISDGITMGTEGMRTSLVSRE
                     VIADSIELVTRGNLFDALIVLVGCDKTIPGGIMALARLNIPGLILYGGSIAPGQFEGH
                     AVTIQDVFEAVGMHARGGMTDAQLASLEGAACPGAGACGGQFTANTMALVCEFLGIAP
                     MGLSSVPATNAAKANAGHRAGELLLDLLRRDVTPSKIITKAAIENAIAGVAATGGSTN
                     AVLHLLAIANEAKIPLSIEDFDRISSRVPILADLKPGGRFVATDLYAAGGTALVAKRL
                     LEAGLLRGDCITVTGRTLAEEAAAAKEAPAQQVVRQLDSPLKPTGGLVILKGNLAPEG
                     CVVKVAGHNLQNFRGPARVFDNEEAAFTAVEKSIIKVGDVVVIRYEGPKGGPGMREML
                     AVTAALVGAGLGDSVALLTDGRFSGATHGLMAGHVAPEAVNGGPIAALADGDTIVFDI
                     PNRQLN"
     gene            2029..3135
                     /locus_tag="VGR03_13525"
     CDS             2029..3135
                     /locus_tag="VGR03_13525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1995344.1"
                     /translation="MREGTGVLVIGGGPAGLAAAIAARMKGFEVTVADGAKPPIDKAC
                     GEGLMPSTMAALRELGVAICPGNGQVLRGVCFKDAATSVEASFSGASGFGVRRTVLHQ
                     KMVDRAQECGITLLWNTPVAGLTSEGAILGDTAMKARWIIGADGIHSRVRRWIGLKAK
                     ARQEMRFAQRRHFRVKAWTDCMEIHWGQKSQAYVTPLSNDETCVVLISRDPRIRLEDA
                     WRKFPSLASHLCHAEPSSAERGAVTVTRRLGQVYRGNIALTGDASGSVDAITGEGLCL
                     SFHQAIALADALGKGKLESYQRAHRRLARRPNTMGRLLLLLDRYPSLRKRALRGLAEE
                     PELFARLLAAHLGEASPKFLAAMSLRLGWQFLTA"
     gene            3152..3784
                     /locus_tag="VGR03_13530"
     CDS             3152..3784
                     /locus_tag="VGR03_13530"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEV1995345.1"
                     /translation="MNWSRSRLAAVFYFLAAAPVTLVVLSALPQHAAPQPPASEIVLG
                     IDPAQSKVHWTLGSTLHTVHGTFALKKGTVHLDPASGKAGGEIVVDATSGNSGNDSRD
                     KKMHKEVLESGQYPDVIFRPDRVEGKITPQGTFTVQVHGIFVLHGNEHELTVPVQAEL
                     AGDHWTGSAKFSVPFIDWGLKNPSSFLLKVNHAVEIELELKGSLQNPAAP"
     gene            complement(3792..4919)
                     /locus_tag="VGR03_13535"
     CDS             complement(3792..4919)
                     /locus_tag="VGR03_13535"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HEV1995346.1"
                     /translation="MPKKPEDWLEIASPPQKSRGIFKLFLGYAPGVGKTFSMLSEALR
                     RRNRGEDVVIGVVETHGRKGIEELLTNIESVPRKKIEYKGTIFEEMDVDAILARHPQV
                     ILVDELAHTNIPGSKHRKRYEDVQELLAAKIDVVSTLNIQHIENIAPVVRSITGITVR
                     ETVPDWVPLTASETVMVDLTPEALQNRMKRGDVYSSEKVERSLKNFFRRGNLIALREL
                     ALRQVAEQVDRSLETYMEAKDIRKNWAVRERMAVCISSNPSGQYLIARGARMARRMDA
                     ELYVIHVDREFDARESNQNALNANLRFAESLGAQVVRLKGRSVADSVAEFVRSKHITH
                     VIFGRAAIHDWRKYLYLSAVHRFLRESPAVDVHIVTRESEE"
     gene            4969..>6399
                     /locus_tag="VGR03_13540"
     CDS             4969..>6399
                     /locus_tag="VGR03_13540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEV1995347.1"
                     /translation="MQRFLAGRTFGFALSIIIVAGIAFLYRHVLLVNQTTVALTLLLA
                     ILAVSAVWGMAVSVFMSVVAMLVFNYEFLPPVGNLTIADPQNWVALGAFLVTSIMGSQ
                     LSARIRREADEAHSRRREIERLYKFSQKLLGEGNVIQLMNAIPNYLVESFEAGAAELF
                     LPQKEKFYRSGYGASHLDEEKMKTAFLRDEMTIEPQQSLYFVPVRLGVKAIGSLGISG
                     ASLSRQTLDAVGSLVAIAIERARAVEQLSLSEAERQGERLKSALLDSVTHDFRTPLTS
                     MKAAVTSLLASGEVAVPQGHELLSIINEECDRLNHLVEEAAEMARLEAGEIELHYAPT
                     SIEEIIQGALALSKSALGAREIHVKVAPDLPDVEADLDRAQDILVKLIDNANLYSSKE
                     QPIIITAELSGDYVTTSVADRGPGIDDLEQGMIFDKFYRGKDQRYLVRGTGMGLPIAK
                     AIVTAHKGTIGVTSQLGHGSVFSFSLP"
BASE COUNT         1372 a         1727 c         1960 g         1340 t
ORIGIN      
        1 cattcaattg ccggttggga atgtcgaaca cgatggtgtc accgtccgcg agggccgcta
       61 tcgggccacc gttaacggct tcgggagcga cgtgtccagc catcaggccg tgcgtggcgc
      121 cggagaaacg gccgtcggtg aggagagcaa cggaatcgcc gaggccggcg cccaccagag
      181 cggccgtaac cgcgagcatt tcacgcatgc ccgggccacc tttcgggccc tcgtagcgaa
      241 tgacgactac atctccaacc ttgatgatgc tcttttcgac ggcggtgaaa gctgcctctt
      301 cattgtcaaa cacgcgagcg gggccgcgga aattctgcaa attgtggccc gccactttta
      361 cgacgcagcc ttccggggcg agatttcctt tgaggatgac gagtccgccg gtcggcttga
      421 ggggcgagtc gagctggcgg acaacctgct gcgccggcgc ttccttggca gcggctgcct
      481 cttccgcgag agtgcgcccg gtaacggtga tgcaatcacc gcgaagcaat ccagcttcaa
      541 ggaggcgctt tgcgacgagc gcagtgccgc ctgctgcgta aagatcggtt gcgacaaaac
      601 gtcctccggg tttgagatcc gcgagaatgg gaacgcgcga gctgatgcga tcgaaatctt
      661 cgatggaaag cgggatcttc gcttcgttgg cgatggccag caaatgcagc acggcattcg
      721 tcgagccgcc tgttgcggcg acaccggcga tggcattctc gatcgcggct ttagtgatga
      781 ttttcgaggg agtgacatcg cggcgaagaa gatcaaggag taattcgcct gcacgatgac
      841 cggcgtttgc tttagcggca tttgtggcgg gaacactcga aagtcccatg ggtgcgatcc
      901 cgagaaactc gcagaccaat gccatggtgt tcgcggtgaa ttgtccgccg caggcgccgg
      961 ctccggggca tgcagcgcct tccagcgacg caagctgagc gtccgtcatt ccgccgcgag
     1021 cgtgcatgcc gacggcttcg aagacgtcct gaatggtgac ggcatggccc tcgaactggc
     1081 cgggagcgat cgagccgcca tatagaatga gcccgggaat attcaagcgg gccagggcca
     1141 ttatgccgcc gggaatggtc ttgtcgcagc cgacgagaac aatcagggca tcgaacagat
     1201 tgccgcgggt caccagctcg atcgaatcag caatgacttc gcggctgacc agagacgtgc
     1261 gcatgccctc cgtccccatg gtgatgccgt cggagatgga taccgtgttg aactcgagcg
     1321 gagtgccgcc cgcggcacga atcccttcct tcacatgctg cgcaagctcg cgaaggtgat
     1381 agttgcacgg accgatctcg atccaggtat ttgccacgcc cacgagaggc ttcttgaagt
     1441 cctcatcgcg aaggccggaa gcacgcagca tggcgcgggc cggcgcacga tgcgccccgt
     1501 ccgtcagagt gtggctgcgc ggttttagca aggagtcact cactgcagag tcccttcgat
     1561 ggtcgtcaac tgcttccatc ctgaaaatta caaaccccga aaaaacaagg ccatcatcca
     1621 gatctgaatg atggccccga gagcttccgg tggtgctgtc ctaggccgcc atcatccgcc
     1681 tgttgtcccc cttacgggaa cgggaataat tcgcacgaca attgggtcta cgattcgggc
     1741 agttttatcc ttcgcgagaa tccgatcgtt tgcgatggcg aggcaaaaca taagtgttca
     1801 ccatcaaact agcaggatgg ggcggggaag tcaatgagtc aaattaatag ttatgctgaa
     1861 tcaaataaat acagcttatg caagggtctt ccgggctgaa cattctgcgc cctgtttggg
     1921 cagacgcacg gcgaaagagc ggagctggcg ggcatcttct ctggcgtttc cacatctaac
     1981 taagatacgt ttcattcacc cagagcagaa gcgatccaag gagactgcat gagggaaggc
     2041 actggtgtgc tggttatagg tggggggccg gccggattgg cggcggccat cgctgcgcga
     2101 atgaagggtt ttgaagttac cgtcgcggac ggcgcgaagc ctcccataga caaggcttgt
     2161 ggtgaaggcc tgatgccgag caccatggca gcactgcgcg agctaggcgt cgcgatttgt
     2221 ccgggcaatg ggcaggtttt gcggggagtc tgcttcaaag acgcggcgac ctcggtggaa
     2281 gcgagctttt ccggtgctag cggttttggc gtgcgtcgaa cagtcctcca ccagaagatg
     2341 gtggatcgag cgcaagagtg tggaatcact ctgctatgga acacacccgt tgcgggcttg
     2401 accagtgagg gcgcgatcct tggtgatacc gcaatgaagg cgaggtggat cattggagca
     2461 gatggaattc actctcgcgt gagacgttgg atcggcttga aggcgaaagc gcggcaagaa
     2521 atgcggtttg cgcagagacg acattttcga gtgaaggcgt ggaccgattg catggagatt
     2581 cattggggcc aaaagtcgca agcgtacgtc acgccactca gtaacgacga gacatgcgtc
     2641 gtgctgattt cgcgcgatcc gcggatacgt cttgaggatg cctggaggaa atttcccagt
     2701 ttagccagcc atttgtgcca tgcagaacca agcagcgcgg aacgaggcgc cgtgaccgtg
     2761 acgcgcagat tgggccaggt ctaccggggg aatattgcat tgacaggaga cgcgtccgga
     2821 agtgttgacg ctatcaccgg ggaaggactt tgcttgagct tccaccaagc gatagcgctc
     2881 gccgacgcat tggggaaagg aaagcttgag agttaccaac gagcgcaccg ccggcttgca
     2941 aggcggccga ataccatggg acggctcttg ctcttgcttg atcgatatcc gtccttgaga
     3001 aaacgcgcgt tgcgaggttt ggccgaggag ccggagctat tcgcgcgact cttggcagcg
     3061 cacctgggag aggcttcccc taaatttctg gcggccatga gtttgcgcct cggttggcaa
     3121 tttctgaccg cgtgaatgga gagggtgaaa gatgaattgg tcccgaagcc gtctggcagc
     3181 ggtcttctat tttcttgcag cagcgcccgt gacgctggtg gtgctcagcg ctctgccgca
     3241 gcatgctgcg ccgcagcctc cggccagtga aattgttctc gggatcgatc cagcgcagag
     3301 taaggtgcac tggactcttg ggagcacgct ccatacggtt catggaacgt ttgcattgaa
     3361 gaagggcacg gttcatcttg accctgcaag cggcaaagcc ggcggtgaga tcgtggtgga
     3421 cgcgaccagc gggaatagcg gcaacgattc gcgcgacaag aaaatgcata aagaggttct
     3481 cgaaagcggg cagtacccgg atgtcatttt tcgaccggac cgcgtggaag ggaagattac
     3541 gccgcaggga actttcacgg tccaggtgca cggaattttt gttttgcacg gcaacgaaca
     3601 cgaactgacc gtgcctgttc aagcggaact tgccggagac cattggacgg gcagcgcgaa
     3661 gttcagcgtg cccttcatcg attggggcct caagaatccg agcagcttct tgctcaaggt
     3721 aaaccacgcg gtagagatcg aactggaatt gaaaggcagt ctgcaaaacc ccgcggcgcc
     3781 gtaatccaaa gctactcttc agattctcgc gtgacgatat gaacgtccac cgcgggcgat
     3841 tcgcgaagaa aacggtgcac ggccgataga taaaggtact tgcgccaatc gtgaatcgcg
     3901 gcccggccaa aaatcacgtg agtgatatgt ttcgagcgga caaattccgc cacactatcg
     3961 gcaacgcttc ggcctttcag acggaccacc tgagcgccga gactttccgc gaaacgcaga
     4021 ttggcattga gggcgttctg gtttgattct ctcgcgtcga attcgcggtc cacgtgaatc
     4081 acgtagagtt ctgcatccat gcggcgcgcc atgcgagccc cgcgagcgat caggtactgt
     4141 cccgaaggat tcgatgaaat acagacggcc atgcgctccc gcaccgccca gtttttccgg
     4201 atgtctttcg cttccatgta agtttcgagg ctgcggtcaa cctgctcagc gacctggcgc
     4261 aaggcgagct cgcggagggc aatgagattc ccgcggcgga agaaattctt gagagagcgt
     4321 tccacctttt cggagctgta gacatcgcca cgcttcatgc ggttttgcaa ggcttccggg
     4381 gtgaggtcca ccattacggt ttcggaggca gtgaggggga cccagtccgg aaccgtctcg
     4441 cgcacggtga tgccagtgat ggaacgtacg acgggcgcaa tgttttcgat gtgctgaatg
     4501 ttgagtgtgg acactacgtc gatttttgcg gccaggagtt cttggacgtc ctcgtaccgc
     4561 ttgcggtgct tactcccggg gatattcgta tgggctagct cgtcgaccag gatcacctga
     4621 gggtggcgcg cgaggatggc gtcgacgtcc atctcctcaa agatcgtgcc tttgtattca
     4681 atttttttcc gcggaactga ctcaatattt gtgagaagtt cttcgatgcc tttgcggccg
     4741 tgagtttcca cgactccgat tacgacgtct tcgccacggt ttctgcggcg gagcgcctcg
     4801 ctcaacatgc taaaggtctt gccaactccc ggagcgtagc cgaggaaaag cttgaatatg
     4861 ccgcgcgact tttgcggggg agaggcgatt tccaaccagt cttccggctt ctttggcaaa
     4921 ggactatctt ctccccggca aatcgaaagc ctgcgctaga ataggtaaat gcagagattt
     4981 ctcgccggcc ggacatttgg tttcgcgctc tccataatca tcgtcgccgg catcgcattt
     5041 ctttaccgcc acgtccttct tgtgaatcag actacggtcg cgctgactct tcttctcgca
     5101 attctagcag tttcggcggt ctgggggatg gcggtgtccg tcttcatgtc cgtggtggct
     5161 atgctggtct tcaactatga gttcttgccc cccgttggaa atttaaccat tgccgacccg
     5221 cagaattggg tggccctcgg tgcattcctg gttacatcca tcatgggcag ccagctctcg
     5281 gcgcggatcc ggagagaagc agacgaagcg cacagccgcc gcagggagat cgaacggctc
     5341 tacaaattca gccaaaaatt gcttggggaa ggcaacgtca tccagttgat gaacgcaatt
     5401 ccaaattacc tagtggaaag tttcgaggcg ggtgccgcgg agttattcct cccgcagaaa
     5461 gaaaagttct accgatcggg gtatggcgcc tcccatctag atgaagagaa gatgaaaacg
     5521 gcgttcctcc gcgatgaaat gaccatcgaa ccgcagcaga gtctttactt tgttcccgtg
     5581 cgcctcggcg tgaaggccat cggaagcctc ggtatttccg gggcgagtct ctctcgccag
     5641 acgctcgatg cggtgggtag cctggtggcc atcgccatcg aacgggcgcg agccgtggaa
     5701 cagctcagcc tgtcggaagc agagaggcaa ggagagcggc tgaagtccgc attgctggat
     5761 tccgtcacgc acgatttccg aacaccgctg acctccatga aagcggcagt gacaagcctg
     5821 ctcgccagcg gggaagtggc cgttccccaa ggtcacgaat tgctgagcat catcaatgaa
     5881 gagtgcgaca ggctgaacca cctcgttgaa gaggccgcgg agatggcgcg gctggaagcc
     5941 ggtgaaatcg agctgcatta tgcgccgaca tcgattgaag agatcattca gggagcattg
     6001 gcgctatcca aatctgcatt gggagcgcgg gagattcacg tgaaagtagc cccggatctt
     6061 ccggatgtgg aggcagatct tgaccgtgcc caggacattt tggtgaagct catcgacaat
     6121 gccaatctgt attcgtcaaa agagcagccg atcattatta ccgcagaact cagcggggat
     6181 tacgtaacga cgagtgttgc tgaccgcggt ccgggcattg acgatctcga gcagggaatg
     6241 atcttcgaca aattctatcg cgggaaagat cagcgctacc ttgtgcgcgg caccggaatg
     6301 gggttgccga tcgcgaaggc gattgtgacg gcccacaagg gaaccattgg agtgacgagc
     6361 cagctcggtc atggctcggt cttctctttt tcccttcct
//
LOCUS       DASYVO010000407        20467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_39830, whole genome shotgun sequence.
ACCESSION   DASYVO010000407 DASYVO010000000
VERSION     DASYVO010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 20467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20467
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39830"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VGR03_13545"
     CDS             <1..756
                     /locus_tag="VGR03_13545"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1995348.1"
                     /translation="LFPYSQTVGALATWLCRTNAALAVFNLLPGFPLDGGRIFRAIVW
                     GATKNFEKATRVAGASGKIIAYAMILSGAYGVFNGFVQQGLWIAFIGWFILNAAQESV
                     AQVAIRETLAGLSAADVMSKEVPTAPGHITLEEYSAEVLRTGRRVHLVVSDDRLLGMM
                     NVHTLNSVPRDEWTHNSVQAAMIPREKILWTSPEEPLLRLLERLLSADVNQMPVVSGA
                     IDGAPQIVGIVTRDSILRVMQTRSELGPLATSR"
     gene            complement(816..2210)
                     /locus_tag="VGR03_13550"
     CDS             complement(816..2210)
                     /locus_tag="VGR03_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV1995349.1"
                     /translation="MWRATRKKFPAGPSEGLKRWSLGPLNNNPLEYFTKIAREYGDIA
                     GLRVLNFKTIFINHPDMIEEVLVTNARKYSKGRVLRANRHVFGEGLLTSEGDFWLRQR
                     RLAQPAFHKARIASYAATMVEYTQRMLDGWRGGEERDAHQEMMRLTLQIVGKTLFDAD
                     VENDAQEVGKSLELLLEIGADFRRAIFVPHWLPTPANLRVKREVAQIEKILYRIISER
                     RASGRDASDLLSMLLSAQDEDGSRMTDRQLRDEAITLFLAGHETTASTLSWTWWLLAQ
                     NPAVEAKLHAELDAVLGERAPSLDDLPKLVYTGHVITESLRLYPAAWGMARLVIEDHE
                     IAGYPVTKGMGVAMAQWVVHRDPRWYDAPEVFRPERWEDDLMKRLPRIAYFPFGGGPR
                     QCIGNTFALMEAALILATIARKFRLRLVANHPVVPLASITLRPRHGVRVTLESRQQND
                     TQKSFTGERSAVAD"
     gene            complement(2267..2839)
                     /locus_tag="VGR03_13555"
     CDS             complement(2267..2839)
                     /locus_tag="VGR03_13555"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1995350.1"
                     /translation="MNAYETWFCGSWFWRWVTRRQLLPWILQGSELGEHLLELGAGPG
                     AATDELLLRAARVTSLEYDHAFTAKLGARMSSANVTVIQGDAATLPFSDGTFSSAIAI
                     LMLHHLRSGELQDRAFAEICRVLRPGGVFLAFEIQDGWLHRVGHVRSTFVPVTPASAF
                     ARLTAAGFSKVTVDFRRGGFRIRALRARET"
     gene            complement(2909..4126)
                     /locus_tag="VGR03_13560"
     CDS             complement(2909..4126)
                     /locus_tag="VGR03_13560"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEV1995351.1"
                     /translation="MKPIAGSRILQNEYSTWIIQDKHSRAYQALTGDVGERMARILRL
                     GAKAEEAPKCLTCHSLNPPPEQRGRAFEIAEGVSCENCHGPASGWLGPHTTRAWAHEK
                     SVALGMHDTRDVIHRTEKCLECHLGTKNKFVDHEMIAAGHPDLYFELDSFSAVMPRHW
                     KTPRESEPGKPVEDAAWVGVREWSAGQAVQLRAAMERLAWRAKNERGDKKDVWPEYSE
                     LSCFACHHALGPAKDSWRQEHGYIGRRAGDPAWNSSRYAVFRLLAKQIDSANAQELDR
                     QLLAVSDEMSKLNPDRNAVASAALVAAPLAQRIAERLATMQYEQAVALRMLQRIPDDA
                     ENIALADERAAEQAAMALDSLYIAYSKDAKPANAAEVRAAINGLFQQLENPSSYNADQ
                     FASALRRIRPMLQ"
     gene            4167..6203
                     /locus_tag="VGR03_13565"
     CDS             4167..6203
                     /locus_tag="VGR03_13565"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEV1995352.1"
                     /translation="MYLMGPFWAESSGGNVTSNAHKTTRKMQEMEVSRIWCADESVCL
                     YRLRRAEVNGPSYLFRAAPRKDKRVLFTADSPASSMQGIQHATSRRASFENMPGAFHA
                     RRGAVIFDDLRAIQLRYGFLPKAELEGLSQRTQTPLYQIHSVASFYPHFHLVPPPKAE
                     IRVCADMSCHLNGACELRADLERRFANSRPEDVQVRDVSCLGRCDHAPAISINDHIFT
                     DVTAAHAEQIARGAIRGDDIHEPHPEQARIACKADPYGEGEKYGVVRKLIQSKAWDDV
                     LAQLKAAELKGMGGAGFPTSMKWDLVRKQPDPVRYIVCNADESEPGTIKDRFIMTNLP
                     HLLIEGMMIAGLVTGAKRGILYIRHEYHLQEEILGEEIRRCYRSGLLGKKILGSDLDY
                     DLEVFVSPGGYICGEESALIEAIEGHRAEPRNKPPFPGQIGLWQKPTVINNVETFINV
                     PQILARGIDWFVAQGAGGSRGLKFVGVSGHVARPGIFEVSMGVPMRDVIFNHAGGVRG
                     GRQLKAFAPSGPSSGYLPASMIDVRLDFKALAEAGSMLGSGAIVVCDDTTCMLDMALN
                     AVRFYRNESCGKCVPCRVGSQKMADILVRWTQGGLPEPQYRADLALLDELSQAMSLTS
                     ICGLGQIVPAPIQSVLKHFRTEIDAHVLKSQCSSGICFTAASRAGEAQRVGIRP"
     gene            6205..8376
                     /locus_tag="VGR03_13570"
     CDS             6205..8376
                     /locus_tag="VGR03_13570"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEV1995353.1"
                     /translation="MTTATTPPGLVDITLDGQPVRIPAGTSVFDAARMNGINIPTLCH
                     LQNQTPVGVCRLCVVDTGARVLAASCVRPVEPGMKVVTNSEKVLAARKTVLELMMSDH
                     PSPCARQQNSGDCELETLAKAAGASQPRFAKRTVARGKDDSSLAIAVDHDACILCDRC
                     IRGCDEVKSNFVLGRMGKGYSAGIAFDLNSPMGDSTCISCGECMVSCPTGALTNKSVV
                     GTAIAVGPDAQGFEAEDLLQIPVFKGVSGTFLELNRGAIVKHRYHKGEIICREGEFGS
                     TAFYILEGQAKVSISTPIAHVKTQGGAKGFFKRLTSTLVAREEDKRAEETRDRTIPID
                     ASVDLAYGNPVAELGPGDLFGEMTCMNFYPRSATVIAESDVVAFEMLRNVLDIMLKNK
                     TFRAQLDQTYRRRALENHLRGVPMFADLSPEFIAHLKESVELQRFAPGQVIVQQGASA
                     DSFYLVRIGFVKVSENYPGGELVLAYLSRGDYFGEIGLLGGGVRTATCTALDHVELVQ
                     ISGDDFRDMVARFPSVRTGLEAVAAERHAANQQRMQMVHNVPIDQFLSQGLMEAQSLL
                     ILDLQNCTRCDACVNACADAHDGITRLVRDGLRFDQYLVATSCRQCRDPLCMVGCPVG
                     SIRRRNSLEVIIEDWCIGCGLCAQNCPYGNINLHPFEVMVDDPERAGRKKAAIKQKAT
                     SCDLCTHLKEPSCVYACPHDAAHRVDPRTFFAEMLGQGPAK"
     gene            8379..9353
                     /locus_tag="VGR03_13575"
     CDS             8379..9353
                     /locus_tag="VGR03_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995354.1"
                     /translation="MDRTQRGWALASLTFLAISAVVYVLYAFKSPQGPRGGSTIGLAF
                     GVIGFGFMIFAALLGARKRVPTWRIGRAQAWMRGHLWLGFLALPMILFHGGFHFGGTL
                     TRVLMWLLIITVFSGVFGAALQHYVPRVMTSDVPLETIYDEIGHVRSLLREEADRAVE
                     SLCGSMGLSKTPDGEVRRAGGFTALRTMAASAVPLRTSAAVSAGASAAVAAAPEIILL
                     SEEESAPLRRFYLEDMRPFLEHLERRGQRLGDAQKAASAFAGLRTLLPAAGHTALGDL
                     ADICDEARQLQRQARLHHWLHGWLLVHIPLSLALILLGAIHAVMALRY"
     gene            9368..10900
                     /locus_tag="VGR03_13580"
     CDS             9368..10900
                     /locus_tag="VGR03_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995355.1"
                     /translation="MARTRTTKKLAQRIDLNYFKRPTPLKRAKFWLSLLLPLLALLWI
                     TEGTIFKDSRVYSSGRLSEPHAVLEKECATCHTQQAGMFSAKAGDNACLSCHDGPTHH
                     EAASKKLECAACHTEHRGRINIAAASNQSCAECHADLRVSKGTTEYAKNIRSFEKGHP
                     EFAALRIDGPTSGRDLGTIKLNHGIHMKPIRRGPTGPIVNLECGNCHRPIADQTVLME
                     YDARYAKPLMNWPYADHVSAKTTYAAPEDVASDPWDLLHPSHSRERERMAPVKFAKAC
                     ADCHSLAFDKRFREGVPHDQPEVIHDFVVSKFRDYIHAHPEELRVQRDPDRDISGKPM
                     QPPVRVFTPAQWVAERTAEAEFLLWRKTCVQCHTLGKWSPRAIEAKHHIPYPPMTLDD
                     LRHFVETFNRPELFLEPIYAQIPQIWPAGVTLRWMPHAKFDHEAHRPFSCVSCHAKAL
                     TSTETSDILLPGIETCKTCHAPGPEHAESRCFECHTYHDWSKRKEVTPTFTLPALRTG
                     GH"
     gene            complement(10967..11956)
                     /locus_tag="VGR03_13585"
     CDS             complement(10967..11956)
                     /locus_tag="VGR03_13585"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV1995356.1"
                     /translation="MEKRQLGKNGPLVSAMGLGCMGMSEFYGARDDAESIATIHRSVE
                     LGITFLDTADVYGYGDNELLVGQAIRGLGDKVFLATKFGIVRDKTNPAVRGVNGRPEY
                     IRESCDASLKRLDVESIDLYYQHRVDPTVPIEETVGAMAELVHAGKVRHLGLSEASAS
                     TIRRAHKVHPIAALQTEYSLWSRDPEDEILATARELGIAFVAYSPLGRGFLTGQIKRF
                     EDFAPDDYRRNSPRFLGENFQKNLDLVARIESIAKEKKCTPGQLALAWLLAQGEDIIP
                     IPGTKRRKYLEENVSGLDLKLSAEDLRRIDEVAPRGAAAGLRYPEQMMKSVGR"
     gene            complement(12182..13705)
                     /gene="xylB"
                     /locus_tag="VGR03_13590"
     CDS             complement(12182..13705)
                     /gene="xylB"
                     /locus_tag="VGR03_13590"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEV1995357.1"
                     /translation="MPTVEGGPIYVLGIDVGTGGTRAIIIDGNGRIVSSATEEHQPFA
                     SPQIGWAEQQPEDWWRASCIAVRKALASANLSAEEIACVGFSGQMHGAVMLDEQGSVV
                     RPALIWCDVRTEKQCRDLTAKIGRERLIQLTCNPALANFTLTKMLWVRENEPENWKRV
                     RSVMLPKDYVRFRLTGERATDVADASGTLLLDVVHRRWSQEMLQAAEIDERLLPSLHE
                     SPDVCGKISSKGAAEIGLRVGTPVVAGAGDQAAGATGMGIVAAGTVSATIGTSGVVFA
                     ATDCPALDPQGRVHTFCHAIPGRWHVMGVTQAAGLSLRWFRDTFATSSTGARESYDQL
                     TAKAAKIPAGSDGLLWTPYLMGERTPHLDSNARGALVGLTASHTRAHIVRAILEGVAF
                     SLRDTFTLFREMNVPVTSIRLGGGGARSPLWRQIQADVYGHAVEIVEAEEGAAYGAAL
                     LAGVGARVWPSVDAACAAAVRVAARVNPQPTAVATFNASYSVFRRIYPATREIFTPR"
     gene            complement(13733..14941)
                     /locus_tag="VGR03_13595"
     CDS             complement(13733..14941)
                     /locus_tag="VGR03_13595"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEV1995358.1"
                     /translation="MATINTKRGVIGILTGGGDVPGLNPAIRGVTLRALREGYQVIGI
                     RHGWAGLVDMVREKDYDNSDNFFLLDEETVDRAGRTGGTFLHSSRTNPGRVGKSSVPA
                     HLKDKFTAEKNDLTPEVLKNLAWLGIDTLVPIGGDDTLSYGVRLHKEGFKVIGIPKTM
                     DNDVPGTDYCIGFSTCVTRTIQMVNSLRTSAGSHERFMVLEVFGRYAGFTAMLPTMAG
                     AANRCVIPEYKFNIEQLTELLVADRRKNPSRYSIVLVSEGAMFEGGEMIFESESTDAY
                     GHKKLGGIGEIVGEKIRDLSAKFNNGKKIDVINQKLGYLVRCGDPDAIDSIAPMAYGN
                     LALDLLLKNTSGRLVVLKNGHYDNIPLETVTATKKVVNVKEQYNTERLRPQYASFQMR
                     PLFLMTNEVG"
     gene            complement(15068..15649)
                     /locus_tag="VGR03_13600"
     CDS             complement(15068..15649)
                     /locus_tag="VGR03_13600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV1995359.1"
                     /translation="MSKKSILMIVGDYVEDYEVMVPFQALLMVGHTVDAVCPGKKSGD
                     FVPTCIHDFEGDQTYTEKRGHNFALNATFDQIKAEDYDALIIPGGRAPEYIRLNPRVI
                     EIVRYFSMANKPLAAICHAPQVLAAAGVLKGKKCSAYPATGPDVTLAGGTYVDLPMTD
                     AIADGNLVTGPAWPALNSWLAKFLGVLGTRIEL"
     gene            complement(15785..16927)
                     /locus_tag="VGR03_13605"
     CDS             complement(15785..16927)
                     /locus_tag="VGR03_13605"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEV1995360.1"
                     /translation="MRRTCLKFLFPALFCFLPEPLFAQVQLSPKIDLTQPQDYILKRI
                     SSSDPTGANADSRRIESGSTLTLMDAIGPGLLTHIWITVASPDPYHLKKLVLRIYWDH
                     ETAPSVEAPLGDFFGLGLGEYHRWESQLLSVGSDKSLNSFFPMPFQKHARITVSNEGL
                     QRVDAFYFNLDYRAYPRPLLPETLYFHAQFRQAQPNHGWTSDWQSNGDPKVDGKPNFD
                     GKENYVWMDAAGRGHFIGVTMSVLQNQDGWWGEGDDMFFLDGESRPSIAGTGSEDYFL
                     GAWDFGGHPFFYRLYGAPVVGEERAGSKSSVYRFHLDSPITFTKSIHATIEHGHANHR
                     SDNFYSVAYWYQTEPHASFPALPSVDQRIPSPRRVGGPGNSDMVHP"
     gene            complement(17070..17897)
                     /locus_tag="VGR03_13610"
     CDS             complement(17070..17897)
                     /locus_tag="VGR03_13610"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1995361.1"
                     /translation="MTKQFLLASDFDQTLSFNDSGVVLSELIGFHGFHDKVKGLSEMN
                     LVQQGAELSYLILHDPDFRKVRREHLVETGKHIRLKHDVALFARALDNLAEGYKFHFN
                     VISAAPQEIIQSALEGIVPPDHIFGTRFRYNEATGEVDSIIRASAGWGKITVLEELRA
                     SLGISHDSIIYMGDGSSDIPVMMHVNQYDGLTIAVSEAKFITRVAKRTVLSDNAMSVL
                     VPVLEKVLRWDSARIRRLFASYGLTLLEWDKMRTDRLTIQDSTEHVSVASAPQLNAP"
     gene            complement(18193..>20467)
                     /locus_tag="VGR03_13615"
     CDS             complement(18193..>20467)
                     /locus_tag="VGR03_13615"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995362.1"
                     /translation="QIAFEDPDRFGKAVHLADIHLEKGMQCNDCHFAQDNHGNGKIYG
                     EPRAAVEIDCIDCHGTIRKKATLVTSGPAAPEGGRHLDALRTPWGLRRFEWRGDKVVQ
                     RSMSEENKEWEIVQTIDTITPGNVHFSEKSLRAKLMNKDGVVSSQTPSDDAALAHANS
                     SMTCYSCHTSWTPTCFGCHLQMTANARKPMLHNEGLVTKNYTSYNFQVLRDDIYMLGV
                     DGTVTGHRIAPARSSCAVLVSSQNANRDWLYYTQQTISAPGFSGQAFSTFVPHTVRAK
                     ETKQCSDCHVSSQNDNNAWMAQLLLQGTNFMNFMGRYVYVATGKKGFEAIAVAEHDEP
                     EAVYGSALHRIAYPGNYKKFLVHHRELSAAAEHPGNVLDIQARGEYAYAALGPGGLRV
                     YDIANIDNKSFSEKVTTAPVSPIGQKFYVPTKNALAVASPTTLGVDPLRQQLPENEEQ
                     PIHLLYGFLYVADKEEGLVIIGDPNLKSKSPGVGTLLDGNPANNFLKRAVTFNPNGAL
                     TGSRRITIAGTFAYILTDKALVVVDLDNPLAPRITATIGSPALNDPRGIAVQFRYAFV
                     VDRDGLKVLDVTDLAQPRPITNALVPLEDARNVYVARTYAYVSAGKQGLAIVDVEKPD
                     APKLDQLFTAEGQLNDVNDVKIGMVAASAFAFVADGKNGLRVLQILSPWDDPAHFSGF
                     SPRPTPKLIATARTRGPALAISKGIDRDRAVDESGNQLAVFGRRGARPLNRAESQALF
                     LRNGQLYSVADSPAIHP"
BASE COUNT         4117 a         5996 c         6189 g         4165 t
ORIGIN      
        1 ctcttccctt atagccagac ggtcggagcc ctggccactt ggctctgccg gaccaatgcc
       61 gcgctcgccg ttttcaatct cttgccggga tttcctctgg acggcggaag aatcttccgc
      121 gccatcgttt ggggagcgac caagaacttt gagaaggcga cgcgtgtcgc gggcgccagc
      181 ggcaaaatca ttgcctacgc gatgatcctc tccggtgctt atggtgtctt taacggcttc
      241 gttcaacagg ggctctggat cgcgttcatc ggctggttca ttttgaacgc cgcgcaagag
      301 agcgtcgctc aagtggccat ccgcgaaacc ctggctggcc tcagcgccgc cgacgtcatg
      361 agcaaggaag tgcccaccgc tcccggacac attacgctcg aagaatacag cgccgaagtg
      421 ctccgcaccg gccgccgcgt tcatctcgtg gtcagtgacg atcgcctcct cggcatgatg
      481 aacgttcaca ccctcaactc cgtcccacgc gacgagtgga cccataactc cgtccaggcc
      541 gccatgattc cgcgcgaaaa aatcctttgg acttcgccgg aggaacccct cctgcgtttg
      601 ctcgagcggc tcctgagtgc ggatgtcaat cagatgcctg tcgtgagcgg cgccatcgac
      661 ggcgcgcccc agatcgttgg catcgtcacg cgcgattcca tcctccgcgt gatgcagacg
      721 cgctccgaac tcgggccctt ggccacaagc agatagttag ctgtttcgga gctgaaagtt
      781 tttcgttgtg attcgttctt cggaaaggaa atcagtcaat ccgcaacggc cgatcgttcc
      841 ccggtgaatg acttctgcgt atcgttttgc tgccgcgatt ccagcgtgac gcgcactccg
      901 tgccgcggac gcagcgtgat cgacgccagc ggcaccacag gatggttcgc caccagccgc
      961 aatcgaaact tccgcgcgat cgtcgccaga atcagcgccg cttccatcag cgcaaacgta
     1021 ttcccgatgc actggcgcgg accgccaccg aacggaaaat aagcgatccg cggcaatcgc
     1081 ttcatcaaat catcttccca gcgctccggc cggaatactt ccggtgcgtc gtaccatcgc
     1141 gggtcgcgat gcaccaccca ctgcgccatg gccacgccca ttccctttgt cacgggatag
     1201 cccgcaattt catgatcttc gataaccaga cgcgccattc cccacgctgc cggataaagc
     1261 cgcagcgact ccgtgatgac gtgcccggtg tagacaagtt ttggcagatc atcgagtgaa
     1321 ggagcgcgct cgccgaggac ggcgtccaat tccgcatgca gctttgcttc cacggcggga
     1381 ttctgggcca gcagccacca cgtccaggaa agcgtgctcg ctgttgtttc gtgccccgcc
     1441 aggaacaaag taatcgcctc atcacgaagc tgccgatccg tcattcgcga accgtcttcg
     1501 tcctgagccg agagcaacat ggacagaaga tcgctggcgt cgcggcccga cgcgcggcgc
     1561 tcacttatga ttcggtaaag aattttttcg atctgcgcca cttcccgctt cacgcgcaaa
     1621 tttgctggcg tgggcagcca atgcggcaca aagatcgcgc gccggaaatc agcgccgatt
     1681 tccagaagca gttcgagcga tttccctact tcttgcgcgt cgttttcgac atcggcgtcg
     1741 aacaatgttt ttcccacgat ctgcaacgtg agccgcatca tctcttgatg cgcgtcgcgc
     1801 tcttccccgc ctcgccagcc atccagcatc cgctgcgtat actccaccat cgtcgctgcg
     1861 taagaagcga ttcgcgcttt atgaaacgcc ggttgcgcca gccgccgctg gcgcagccag
     1921 aaatctcctt cgctggtgag caagccttcg ccgaatacgt gcctgttcgc ccgcagcacc
     1981 ctacccttcg agtatttgcg agcgttggtc accagtactt cttcgatcat gtcgggatgg
     2041 ttgatgaaaa ttgttttgaa attcagcacg cgcagccctg cgatgtcgcc atactcgcgc
     2101 gcgattttgg tgaaatattc cagcggattg ttattgagcg gcccgagcga ccatcgtttg
     2161 agtccttcgg aggggccggc gggaaatttt ttgcgagtcg cacgccacat ctgatgatgc
     2221 tatgcccgcg tgcgaactgc gtcaacgctg cgcgcgtgtg aagcgctcat gtttctcgcg
     2281 cgcggagcgc gcggattcga aagccgccgc gccggaaatc gacagtcacc tttgaaaatc
     2341 ccgcggccgt cagtctcgcg aacgcagacg ccggcgtcac cggcacaaac gtactccgga
     2401 cgtgtcccac tctgtgcagc caaccatcct gaatctcaaa cgcgaggaaa actccacccg
     2461 gccgaagcac ccggcaaatt tccgcgaagg cgcgatcctg cagctcgccg gaacgtaaat
     2521 gatgaagcat taggatcgca atcgccgaag aaaatgtgcc gtcggaaaaa ggcagcgtcg
     2581 cggcatctcc ttgaatcact gtcacattcg cactgctcat gcgagcgccg agcttcgccg
     2641 taaacgcgtg gtcgtactca agactcgtca cgcgagcggc gcgcaacagc aactcatccg
     2701 tcgccgctcc cggccccgca cccagttcca gtaagtgctc cccaagctcc gaaccttgca
     2761 aaatccacgg caaaagttgc cgccgcgtca cccatcgcca gaaccacgat ccacagaacc
     2821 acgtttcata cgcgttcacg gtctgattct ccaaaacaga ttcttgcgat tgcggtagac
     2881 ggtagcatcg aggcccatag aattgacatc actgaagcat cggccggatg cggcgcaggg
     2941 cagatgcaaa ctgatcggcg ttgtaggacg aaggattctc gagctgctgg aagagaccgt
     3001 ttatagcggc gcggacttcg gcggcgtttg ccggctttgc gtccttgcta taggcaatgt
     3061 atagcgaatc cagcgccatg gctgcttgtt ccgcggcgcg ctcgtctgcc agcgcgatat
     3121 tttcggcatc atcgggaatg cgttgcagca tgcgcagcgc aacagcctgc tcgtattgca
     3181 tcgtggccag ccgttccgcg attcgctgag ccagcggcgc cgccaccaat gccgccgaag
     3241 ctaccgcgtt gcgatccgga ttgagcttgc tcatctcatc ggaaacggca agcagttggc
     3301 ggtccagttc ctgcgcgttc gccgagtcga tctgtttcgc cagtaaacgg aacacggcat
     3361 aacgcgaact gttccatgcg ggatcgcccg cacgccgccc aatgtacccg tgctcctggc
     3421 gccaactgtc tttcgccggc cccagcgcat ggtgacacgc aaaacatgaa agttcggagt
     3481 actccggcca aacgtctttt ttatcaccgc gttcgttctt cgcgcgccat gccaatcgct
     3541 ccatcgctgc gcgcaactgc accgcctggc ccgcgctcca ttcgcgcacg ccgacccacg
     3601 cggcatcctc aaccggtttg cctggttccg attcgcgcgg cgtcttccag tgccgtggca
     3661 tcactgccga aaatgaatcc agctcgaaat aaaggtccgg atgccccgcc gcgatcatct
     3721 cgtgatccac aaatttgttt ttcgtcccga ggtggcactc gaggcatttc tcggtgcgat
     3781 gaatcacgtc tcgagtgtcg tgcattccca gcgcgacgga tttctcatgc gcccacgctc
     3841 ttgtggtgtg cggccccagc cagcccgatg ccggcccatg gcaattctcg caggaaacgc
     3901 cttccgcaat ttcaaaagcg cgcccgcgct gctccggcgg cggattcagc gagtggcacg
     3961 tgaggcactt cggcgcttct tcggctttcg cgcccagtct caagatccgc gccatccgct
     4021 cgccaacgtc tcccgtcaag gcctggtaag cgcgactgtg tttgtcctgg atgatccacg
     4081 tcgaatattc gttctgcaaa atccggcttc ccgcgatggg cttcacgctt ccgtgacagc
     4141 tcgtcgcggc acacgaaccc ggtcctatgt atttgatggg cccattctgg gccgaaagca
     4201 gcggcgggaa cgtcacaagc aacgcgcata agaccacgcg gaagatgcag gagatggagg
     4261 tgtcgcgcat ttggtgtgca gatgagtctg tttgcttata tcgtttgcgc cgcgcggaag
     4321 tcaacgggcc gagttatctt tttcgcgccg cgccgcgaaa agacaaacgc gtgctattta
     4381 cagcggactc ccctgccagt agtatgcagg ggattcagca cgccacttcg cgccgcgcat
     4441 cttttgaaaa catgcccggc gcatttcacg cgagacgggg cgccgtgatc ttcgacgacc
     4501 ttcgggccat ccagcttcgc tacggcttcc tgccaaaagc ggaactcgag ggtctttcac
     4561 aacgcaccca gacgccgctc taccagattc acagcgtcgc gagtttctat ccccattttc
     4621 atctcgtacc gccgcccaag gccgagattc gcgtctgcgc cgacatgagc tgccatctaa
     4681 acggcgcttg cgagttgcgc gccgatctcg agcgccgctt cgccaattcg cgccctgaag
     4741 atgtgcaggt tcgcgatgtc tcctgcctcg gccgctgcga tcacgctccg gcaatttcca
     4801 tcaacgatca catcttcacg gacgtcaccg cggcgcatgc cgaacagatc gcccgcggcg
     4861 ccatccgcgg cgacgacatt cacgagccgc atcccgagca ggcacgcatc gcttgcaagg
     4921 ccgatcccta tggcgagggc gaaaagtacg gcgtggtgcg caagctcatt caatccaagg
     4981 cctgggacga cgtcctcgca cagctcaaag ccgccgaact gaaaggcatg ggcggggccg
     5041 gatttcccac cagcatgaag tgggatcttg tccgcaaaca gcccgatcct gtcagataca
     5101 tcgtttgcaa cgccgacgaa agcgagcccg gcacgatcaa ggaccgcttc atcatgacca
     5161 acctgcccca tctcctcatc gaaggcatga tgatcgccgg cctggtcacc ggcgcgaagc
     5221 gcggcatcct ctatattcgc cacgaatacc atcttcagga agaaatcctc ggcgaagaga
     5281 ttcgccgctg ttatcgctct ggccttctag gaaaaaaaat tctcggaagc gatctcgatt
     5341 acgatctcga agtgtttgtc agtcccggcg gctacatctg tggcgaagaa agcgccctca
     5401 tcgaagccat tgaaggccat cgcgctgagc cgcgtaacaa gcctcccttc cccggtcaaa
     5461 tcggcctctg gcagaaacct accgtcatca acaacgtcga aaccttcatc aacgttccgc
     5521 aaattctcgc ccgcgggatc gattggttcg tcgcgcaagg cgccggtggc tcgcgtgggc
     5581 tgaaattcgt cggcgtcagc ggccatgtcg cgcgccctgg catattcgaa gtgtccatgg
     5641 gcgtgcccat gcgcgacgtt atcttcaatc atgccggcgg cgttcgcggc ggccgtcaac
     5701 taaaagcctt cgcgccctcc ggcccgtctt ccggctatct tcccgcgtcg atgatcgacg
     5761 tgcgcctcga cttcaaggct ctcgctgagg cgggctccat gctgggctcc ggcgccatcg
     5821 tcgtctgcga tgacactacc tgcatgctcg acatggccct gaacgccgtg cgcttctatc
     5881 gcaacgaatc ctgcggcaaa tgtgtcccct gccgcgtcgg ctcgcaaaaa atggccgaca
     5941 tactcgtgcg ctggacccaa ggaggcctcc ccgaaccaca atatcgcgcc gatctcgccc
     6001 tgctcgacga actttcgcag gccatgagct tgacttcgat ctgcggcctc ggccagatcg
     6061 tccctgcgcc gattcaatcc gtgctaaaac attttcgcac cgaaatcgat gcgcacgttt
     6121 tgaagagcca gtgttccagc ggcatctgtt tcaccgccgc atcacgcgcc ggcgaagccc
     6181 agcgcgtagg cattcgccct tagtatgacg accgcaacca caccacccgg cctcgtcgac
     6241 atcaccctcg acggccaacc cgtccgcata cccgcgggca cgagcgtctt cgatgccgcg
     6301 cgcatgaacg gcatcaacat ccccacactt tgccatctgc aaaaccagac tcccgtcggc
     6361 gtctgccgcc tttgcgttgt ggataccggc gcgcgcgttc tcgccgcctc ttgcgtccgc
     6421 ccggtcgagc ccggcatgaa ggtcgtcacc aattccgaaa aagttttggc tgcgcgcaag
     6481 acagtcctcg aactcatgat gtccgatcat ccttctcctt gcgcgcgcca gcagaattcc
     6541 ggcgactgcg aattggaaac actcgcgaaa gccgccggcg cttcccagcc gcgcttcgca
     6601 aaacgcacgg tcgcccgcgg caaagacgat tcttccctgg ccatcgccgt cgaccacgac
     6661 gcctgcattc tttgcgaccg ctgcatccga ggctgcgacg aagtcaaaag caatttcgtc
     6721 ctcggccgca tgggcaaagg ctattccgcc ggcatcgcct tcgatctgaa ttcgcccatg
     6781 ggcgattcca cgtgcatctc ttgcggcgag tgcatggtct cctgcccgac cggcgcgctc
     6841 accaacaaga gcgtcgtagg caccgctatc gctgtcggtc cagacgcgca aggattcgaa
     6901 gccgaagatc tcctgcagat ccccgttttc aaaggcgtct ccggaacctt cctcgagctc
     6961 aatcgcggcg ccatcgtcaa gcaccgttac cacaaaggcg aaatcatctg ccgcgaaggc
     7021 gaattcggtt ccaccgcttt ctacattctc gaaggtcaag ccaaagtttc catttccact
     7081 cccatcgctc acgtcaagac gcaaggcggc gccaaaggat tcttcaagcg gctgaccagc
     7141 acgctcgtcg cgcgtgaaga agacaagcgc gcggaggaaa cgcgcgacag aaccattccc
     7201 atcgacgcct ccgtcgatct cgcttacggc aatcccgtgg ccgagctcgg cccgggcgat
     7261 ctcttcggcg agatgacctg catgaatttt tatccgcgct ccgccacggt gatcgcggaa
     7321 tccgatgtcg tcgcgttcga aatgctgcgc aacgttctcg acatcatgct caagaacaag
     7381 actttccgcg cacagctcga ccagacctat cgccgccgcg ctcttgaaaa tcaccttcgt
     7441 ggcgttccca tgttcgcgga tctttcaccg gaattcatcg cgcacctcaa ggaaagcgtc
     7501 gagctgcagc gcttcgcccc cggccaggta atcgtccagc agggcgcctc cgctgacagt
     7561 ttctatctcg tgcgaatcgg tttcgtgaaa gtttcggaaa actatccggg cggcgaactc
     7621 gttctagcct atctctcccg cggcgactac ttcggcgaaa tcggcctcct cggcggcggc
     7681 gttcggacag ccacttgcac cgcgctcgat cacgtcgagc tcgttcaaat ctcgggcgac
     7741 gatttccgcg acatggttgc gcgtttcccc agcgttcgaa ccggcctcga agccgtcgcc
     7801 gccgaacgtc acgccgcaaa tcagcagcgc atgcagatgg ttcacaacgt ccccatcgat
     7861 caattcctct cgcaaggatt gatggaagca cagagcctgc tgattctgga tttgcaaaac
     7921 tgcacgcgct gcgacgcctg tgtgaatgct tgcgccgacg cgcacgacgg catcacgcgt
     7981 ctcgtccgcg atggcttgcg cttcgatcaa tatctcgtcg ccaccagttg ccgccagtgt
     8041 cgcgacccgc tgtgcatggt gggctgcccc gtcggctcga ttcgaaggcg caattcgctc
     8101 gaagtcatca tcgaggattg gtgcatcggc tgcgggctct gcgcgcagaa ttgtccctac
     8161 ggcaacatca atttacatcc cttcgaagtg atggtcgacg atccggagcg agccgggcgc
     8221 aagaaggcgg cgatcaagca aaaagcgact tcgtgcgatc tctgcacgca cttgaaagaa
     8281 ccaagctgcg tctacgcctg tccgcacgat gccgcgcacc gcgtcgatcc gcgcacattt
     8341 tttgccgaaa tgctcggcca gggcccggca aaatgagaat ggatcgaact caacgcggct
     8401 gggctctcgc atcgctgaca tttctcgcga tttctgcggt cgtgtacgtc ctctatgcct
     8461 tcaaatcgcc gcaaggccca cgcggcggca gcactatcgg cctcgcgttt ggcgtcatcg
     8521 gtttcggctt catgattttt gcggcgctgc tcggcgcgcg aaaacgcgtt cctacctggc
     8581 gcattggccg cgcacaagcc tggatgcgcg ggcatctctg gctcggtttt ctcgcgctgc
     8641 cgatgattct gtttcacggc ggatttcatt tcggcggcac gctcacgcgc gtcctgatgt
     8701 ggctactgat catcacggtg ttcagcggcg tgtttggcgc ggcgctgcaa cactacgttc
     8761 cgcgcgtgat gacttcggac gtgccgctcg aaaccattta cgacgaaatt ggccacgtgc
     8821 gttcgcttct gcgcgaagaa gccgatcgcg cggtcgaatc tctctgcggc tccatgggcc
     8881 tcagcaagac tccagacgga gaagttcggc gcgccggcgg cttcacggca ttgcgcacga
     8941 tggctgcgtc cgcagttccc ctgcgcacgt cggccgccgt cagcgcgggc gcgagcgccg
     9001 ctgtcgccgc cgccccggaa atcattttgc tcagcgaaga agagagcgcg ccgctacgcc
     9061 gcttctatct tgaggacatg cggccgttcc tcgaacatct ggaacggcgc ggccagcgcc
     9121 tcggcgatgc tcagaaggcg gccagcgctt tcgccggact acgaaccctg ttgccggcgg
     9181 cgggacacac tgcgcttggc gaccttgcag acatatgcga cgaagcgcgg cagcttcagc
     9241 gccaggcgcg gctgcaccac tggctgcatg gctggcttct ggtgcatatc ccgctttcgc
     9301 tcgcactgat tttgctcggc gccattcatg cggtcatggc gctgcgctac tagcacaaga
     9361 gaaaaacgtg gcacgcacac gcacaacaaa aaaactcgcg cagcgcatcg acctcaacta
     9421 tttcaagcgg ccgacgccac tgaagcgcgc gaaattctgg ctcagccttc tgctgccgtt
     9481 gctcgcgctc ctctggatca ccgaaggcac tattttcaaa gactctcgcg tctacagcag
     9541 cggccgcctg tccgaacctc atgccgttct cgaaaaagaa tgcgccacgt gccacaccca
     9601 gcaagcggga atgttttccg ccaaggctgg ggacaacgct tgcctctcct gccacgacgg
     9661 ccccacacac cacgaggccg cttcgaagaa actagaatgc gctgcctgcc acaccgagca
     9721 tcgaggtcgc atcaacatcg ctgccgccag caatcaatct tgcgccgagt gtcatgccga
     9781 tctccgcgtt tcaaaaggca cgacggaata cgcaaagaac atccgcagtt ttgaaaaggg
     9841 ccacccggaa ttcgccgcgc tgcgcataga cggacccact agcggacgtg atctcggcac
     9901 tatcaagctc aatcacggca tccacatgaa gcccattcgg cgcggcccca ccggcccgat
     9961 cgtcaatctc gaatgcggca actgccatcg ccccattgcc gaccaaacgg tcctgatgga
    10021 atatgacgcc cgctacgcta agcctttgat gaattggccc tatgcggatc atgtctctgc
    10081 gaagactaca tatgcggcgc ccgaagacgt cgctagcgat ccatgggacc tattgcatcc
    10141 atcacactca agagagcgtg aacggatggc gccggtaaag ttcgccaagg cgtgcgccga
    10201 ttgccattcc cttgcattcg acaagcgatt ccgtgaggga gttccgcatg accaaccgga
    10261 agtaattcat gattttgtgg tgagcaaatt cagagattac atccacgcgc atccggaaga
    10321 actccgcgtg cagcgcgacc ccgaccgcga tatcagcggg aaaccgatgc agccgccggt
    10381 tcgagtattc acgcccgcac agtgggtggc tgaacgcacg gcggaagcag aatttcttct
    10441 gtggcgtaaa acttgcgtgc agtgccatac gcttggtaaa tggtcgccgc gagcgatcga
    10501 agcaaaacac cacattccct atccgcccat gaccttggac gatctccggc acttcgtgga
    10561 aacgttcaat cggccggaac tcttcctcga gcccatttat gcccagattc cgcagatttg
    10621 gcccgcaggc gttacgttga gatggatgcc gcatgcgaaa ttcgatcacg aagcgcatcg
    10681 ccctttctcc tgcgtaagct gtcacgcgaa ggcgctgacg agcacggaaa ctagcgacat
    10741 cttgctgcca ggaattgaaa cctgcaaaac gtgccatgcg ccggggcctg agcatgcgga
    10801 gtcgcgatgc ttcgagtgcc acacgtatca cgattggtcg aagcgcaagg aagtcacgcc
    10861 gacgtttacg ctgccggcgc tccgcaccgg tggacactga cggcagcacg gactttggca
    10921 tgcgcgctca agccaactcc gagtttgccg atccggcagc caatcatcag cggcccaccg
    10981 acttcatcat ctgttccgga tagcgcaaac ctgcggcggc gccgcgcggg gctacttcgt
    11041 cgatgcggcg caaatcttcc gcgctcaatt tcaagtccag gccactgacg ttttcttcca
    11101 ggtattttcg ccgcttcgtg cctgggatcg gaatgatgtc ttcgccttgg gcgagcagcc
    11161 atgcgagcgc gagttggccg ggggtgcatt tcttttcctt ggcgattgat tcgattcgcg
    11221 ccacgagatc cagattcttt tgaaagttct cgcccaggaa gcgcggagaa ttcctgcgat
    11281 agtcgtcggg cgcaaaatcc tcgaagcgtt tgatctggcc ggtcagaaag ccgcggccaa
    11341 gcgggctgta ggcgacgaag gcaattccca actcacgcgc tgtggcgagg atttcatctt
    11401 ccggatcgcg gctccacagc gagtactccg tttgcagcgc ggcgatggga tgcactttgt
    11461 gcgcgcgccg aatggtgctg gcggaagctt cggacaatcc gaggtggcgg acttttccgg
    11521 catgcaccag ttccgccatc gcgcccaccg tctcttcgat gggcacagtt ggatcgacgc
    11581 ggtgctggta gtacaaatcg atggattcca cgtcgaggcg cttcaagctg gcatcgcaag
    11641 actcgcgaat gtattcgggc cggccgttga cgccgcgcac ggctgggttg gtcttgtcgc
    11701 gaacaatgcc gaactttgtc gcaagaaaaa ccttgtcgcc cagaccgcga atggcctggc
    11761 caaccaacaa ttcgttatcg ccataaccgt agacgtccgc ggtatcgaga aaggtgatgc
    11821 cgagttcaac ggaacgatga atcgtggcaa tggattccgc gtcgtcgcgc gctccataga
    11881 attccgacat gcccatgcag cccaatccca tcgcggagac aagtggtccg ttcttcccaa
    11941 gctgacgttt ctccatgaaa ttcacctcac tgtactcatt ttgttggatg aacaaccttt
    12001 ctcttcgggt acaggatcag tggccagtga catgggtctc tctccgccgc agtgaaaatg
    12061 ttccgggaat gcgccgcgtg aagatggcac gccagaccga gcagctccgg tggggaggaa
    12121 gaaagtgata gtgcggcggt attcagcggg gcagacttgc aaagagaaac gtcatgtcaa
    12181 tttagcgggg agtgaaaatc tcgcgggtgg cggggtagat gcggcggaag acggaatagc
    12241 tggcgttgaa ggtggcgacg gcagtgggtt ggggattgac gcgggcggcg acgcgaacgg
    12301 cggcagcgca agcagcatcg acggaaggcc acacgcgggc gccgacgccg gcgagaagag
    12361 cggcgccgta ggcggcgcct tcttcggctt cgacgatctc gacggcatgg ccatagacat
    12421 cggcctggat ctggcgccag agcggggagc gcgcgccgcc accgccgagg cggatgctgg
    12481 tgacggggac gttcatttcg cggaagaggg tgaaggtgtc gcggaggctg aaggcaacgc
    12541 cttcgaggat ggcgcgaacg atgtgagcgc gtgtatgcga ggcggtgagg ccgacgagcg
    12601 cgccacgagc gttggaatcg agatgggggg tacgctcgcc catcagatag ggcgtccaca
    12661 ggagtccatc ggaaccggcg gggattttag cggctttggc agtgagctgg tcgtaggatt
    12721 cgcgggcgcc cgtggaactt gtcgcgaagg tgtcgcggaa ccaacgcagg gagaggccag
    12781 cggcctgcgt gacgcccata acgtgccagc ggccagggat ggcgtggcaa aaggtgtgga
    12841 cgcgaccttg aggatcgagc gcggggcaat cggtggcggc aaaaacgact cccgaggtac
    12901 cgatggtggc gctgacggtt ccggccgcga cgatgcccat gccggtggct ccggcagcct
    12961 ggtcgcctgc tcccgctaca acgggggtac caacgcgcag acctatttcc gccgcgccct
    13021 tgctggagat ttttccgcag acatcgggag actcgtggag ggacggcagc aagcgttcat
    13081 cgatttcggc ggcttgcaac atctcctgcg accagcggcg gtggacgacg tcgagaagca
    13141 gggtgccgga agcgtcggcc acatcggtgg cgcgttcacc agtgaggcgg aaacggacgt
    13201 agtccttcgg aagcatgacc gagcgaacgc gtttccaatt ctcgggttcg ttctcgcgga
    13261 cccacagcat ttttgtaagc gtgaaattag ccagcgccgg attgcaggtg agctgaatca
    13321 gccgctcccg gccgatcttg gcggttagat cgcggcactg tttttccgtg cggacgtcgc
    13381 accagatcaa cgcgggacga acgacgcttc cctgctcgtc gagcataacc gcaccgtgca
    13441 tttgaccgga gaagccgacg caggcgattt cttcagcgct caggttggca ctcgcgaggg
    13501 ccttgcgaac cgcgatgcag gaggcacgcc accagtcttc cggttgctgc tcggcccaac
    13561 caatttgcgg cgaagcgaag ggctggtgtt cttcggtggc agaggagacg atgcggccat
    13621 tgccatcgat gatgatcgca cgcgtccctc cggtaccaac gtcaatccca agtacgtaga
    13681 tagggcctcc ctcgacagtc ggcattgctt gcccaatatg ttgggataaa gactatccca
    13741 cttcgttggt catgaggaag aggggccgca tttggaagct agcgtattgg gggcggaggc
    13801 gttcggtatt gtattgctcc ttgacgttga cgaccttttt ggtggcggtg acggtctcaa
    13861 gaggaatatt gtcgtaatgg ccgttcttga ggacgacgag acggccactg gtgtttttga
    13921 ggaggagatc cagagcgaga ttgccgtagg ccatgggagc gatggagtca atggcatcgg
    13981 gatcgccgca gcggacgaga tagccgagct tttggttgat gacgtcgatt ttcttgccgt
    14041 tgttgaattt ggcggagagg tccctaattt tttcaccgac gatttcgccg atgccgccga
    14101 gcttcttgtg accgtaggcg tcggtgcttt cgctttcgaa gatcatttcg ccgccttcaa
    14161 acatggcgcc ttccgagacg aggacgatgg agtagcggct gggattcttg cggcggtcgg
    14221 cgacgagcag ttcggtgagc tgctcgatat tgaatttgta ttcgggaatg acgcagcgat
    14281 tggcggcgcc ggccatggtg ggaagcatgg cggtgaaacc ggcgtagcgg ccaaagactt
    14341 cgagaaccat gaagcgctcg tgggagccgg cggaagtgcg gaggctgttg accatctgga
    14401 tggtgcgcgt gacgcaggtg ctgaagccga tgcaatagtc ggtgccgggg acgtcgttgt
    14461 ccatggtctt gggaatgccg atgactttga agccctcttt gtggaggcga acgccgtagc
    14521 tgagggtgtc gtcgccgccg atggggacga gagtgtcgat gccgagccag gcgagattct
    14581 tgagtacttc gggagtgagg tcgtttttct cggcggtgaa tttgtccttg agatgcgcag
    14641 ggacgctgga tttgcccacg cggccgggat tggtgcggga agaatggaga aaagtgccgc
    14701 cggtgcggcc ggcgcgatcg acggtctctt cgtcgagaag gaaaaagttg tcggagttat
    14761 cgtaatcctt ctctcggacc atatcgacga ggccggccca gccgtggcga atgccgatga
    14821 cctggtagcc ttcgcgcaag gcgcgaagag tgacgccgcg gatagcggga ttaagaccgg
    14881 gaacgtcacc gcctccagtg aggataccga tgacgcctcg cttggtgttg atggttgcca
    14941 taaatctaag gatcgccctt tcgtgggatg ttgaatagga aagtatcctc ttcggaggag
    15001 tttttaggaa gagtgaaggc gagatttgtt ttttaggatg aatggattca aataattggt
    15061 ttggagacta gagttcgatg cgcgtgccaa ggacgccgag gaatttggcg agccaggagt
    15121 tgagcgcggg ccaggccggg ccggtgacga ggttgccgtc agcgatcgca tcggtcatgg
    15181 ggagatcgac gtacgtgccg ccggcgaggg taacgtcagg gccggtggcc ggataggcgc
    15241 tgcacttctt tcctttgagg actcccgcgg cggcgagaac ttgcggggcg tggcagatcg
    15301 cggcgagggg cttattggcc attgagaagt aacgaacgat ttcgatgacg cgaggattta
    15361 ggcgaatgta ttccggcgcg cgacccccgg ggatgataag ggcgtcatag tcttcggcct
    15421 tgatctggtc gaaggtagcg ttgagagcga aattgtggcc gcgcttctcg gtgtaggtct
    15481 gatcgccttc gaaatcgtgg atgcaggtgg gaacgaagtc gccggatttc ttgccgggac
    15541 agaccgcgtc gacggtatgg ccgaccatga gcagggcctg gaatgggacc atcacttcgt
    15601 agtcttcgac gtaatcaccg acgatcatga gtatggattt cttggacatg agaacctccg
    15661 gaaacgccgt agtgtagcag aaacagaaga tgccgggctt cccgacaaaa gacgtcgggc
    15721 cccccaaact gggggaaagc ccggcgctac actgaaggaa attcatcccc tcgtgcgccg
    15781 cccatcaagg atgaaccatg tccgaattgc cggggcctcc gactcgccga ggactgggaa
    15841 tgcgctggtc gacggaggga agagccggga aggaagcgtg aggctcagtc tgataccagt
    15901 aggccacaga atagaaattg tccgagcgat gattcgcatg gccatgctcg atggtagcgt
    15961 gaatcgactt tgtgaaagtg atgggcgaat ccagatggaa gcgatagacg gacgatttgc
    16021 ttccggcgcg ttcttctccg acgacgggag caccgtagag ccgatagaag aaggggtgac
    16081 cgccaaaatc ccaagcgccg aggaaatagt cttcagaacc tgttccagcg atggatggcc
    16141 gggattcgcc gtcaaggaaa aacatgtcgt cgccctcacc ccaccagcca tcctgattct
    16201 gcaagacgga catagtcact ccaatgaagt gtccgcggcc cgcggcgtcc atccagacgt
    16261 aattctcctt gccgtcgaag ttcggcttcc cgtcgacctt gggatcaccg ttgctctgcc
    16321 agtcggaggt ccagccgtgg ttgggctggg cctgtcggaa ctgggcgtga aagtagaggg
    16381 tttcgggcag gagaggtcta gggtaggcgc gatagtcgag attgaaatag aaggcatcca
    16441 ccctttgcag accttcgttg gaaacggtga tgcgcgcatg cttctgaaag ggcatgggga
    16501 aaaaagagtt taaagatttg tcgctgccga cggagagaag ctgggattcc cagcgatggt
    16561 attcgccaag cccgaggccg aaaaaatctc ccagaggagc ttcgacgctc ggtgccgttt
    16621 cgtggtccca atagattcgc agcacgagtt tcttgaggtg gtagggatcg ggactcgcga
    16681 cggtgatcca gatgtgcgtc agaagtccgg gcccgatcgc gtccatcaat gtaagcgtgc
    16741 tgccggactc gatccggcga gaatccgcgt tggctccggt ggggtctgag cttgaaatac
    16801 gcttgagaat gtagtcctgc ggctgcgtga gatcgatctt aggggacaac tgtacttgcg
    16861 caaataaagg ttccggaagg aaacagaaaa gcgcagggaa aaggaatttc aaacaggtgc
    16921 ggcgcattgg ctctcctcct ctggtccgga ccaaacaggg gaagaaggat cttgctccga
    16981 aagaggaaac aaagaaaggg cggactgacc ctttgattgc actcagggta aaagtccgcc
    17041 ctctacacgg aagagacagg cagcagcgct tagggcgcgt tgagttgggg agcggaggct
    17101 accgacacgt gctcggtgga atcctggatg gtgagccggt cggtgcgcat tttgtcccac
    17161 tcgagcaacg tcaggccgta ggatgcgaaa aggcgccgga tgcgagcgga gtcccagcgc
    17221 agaacttttt caagcacggg gacaaggacg ctcatggcgt tgtcgctgag aacagtgcgc
    17281 ttggcgacgc gggtgatgaa cttggcttcg gagacggcga tggtgaggcc gtcatactga
    17341 ttgacgtgca tcatgacggg aatatcggaa ctgccgtcgc ccatgtagat gatgctgtcg
    17401 tggctgatgc cgaggctcgc gcggagctcc tcgagcacgg tgattttgcc ccagccggcc
    17461 gaggcgcgaa tgatggagtc gacttcgccg gtggcttcgt tgtagcggaa gcgcgtgcca
    17521 aagatgtgat ccggcggaac gatgccttcc agcgccgact ggatgatctc ctgcggcgcg
    17581 gcggagatga cgttgaaatg gaacttgtag ccttcggcga gattgtccag cgcgcgcgcg
    17641 aagagcgcga cgtcgtgctt cagacggatg tgcttgccgg tttcgacgag gtgctcgcgg
    17701 cggaccttgc ggaagtccgg gtcatgcaga atgagatagc tgagttccgc gccctgctgg
    17761 acgaggttca tctccgacag gcccttgacc ttgtcgtgga agccgtgaaa gccgatcaat
    17821 tcactgagaa cgactccgga gtcgttgaag ctgagcgtct ggtcgaaatc gctggccagt
    17881 agaaattgtt tggtcatgta ctagtacccc tgttgctgcc tttcctggtg ggcctgctct
    17941 gggaagcctc ctcaccctct ctggatacac cttagctgcg ttcccccaac actgccccac
    18001 gaattatttt gatgggtgct atgcgttaaa gatgtgaacg attcattata ccttgattag
    18061 atgctggctg gctataaatg attaaaagga aaggagatgg ttgtgccaca tagcaacaga
    18121 atgacgcaaa ttcagtgctg tgaaagagat agagtaggcg aagaaagaga gaaagcggcc
    18181 cggggggcgg agctaagggt ggatggcagg tgagtccgcg acggaataga gctggccgtt
    18241 gcgcaggaag agggcttggg attcggcgcg gttgaggggg cgggcaccgc ggcggccgaa
    18301 gacggctagt tggttgccgg actcatcgac ggctcggtca cggtcaatac ccttggaaat
    18361 ggcaagagca gggccgcgcg tgcgagctgt cgcgatcagt ttaggtgtcg gacgcgggct
    18421 gaagccggag aaatgcgcgg ggtcatccca gggagaaagg atctggagga cgcggaggcc
    18481 gttcttgccg tcggcgacga aggcgaaggc gctggcggcg accatgccga ttttgacgtc
    18541 gttgacgtcg ttgagctggc cttcggcggt gaagagttgg tcgagtttcg gtgcgtccgg
    18601 tttttcgacg tcaacgatgg caaggccctg cttccctgcg gagacgtagg cgtaggtgcg
    18661 ggcgacgtag acgttgcggg cgtcttcgag gggaactagt gcgttggtga ttggtcttgg
    18721 ctgcgcaagg tcggttacgt ccagaacttt cagaccgtcg cgatccacga cgaacgcgta
    18781 gcggaattga acagcgatgc cgcgaggatc gttgagcgcg ggcgagccga tggtcgcggt
    18841 gatacgcgga gcgagcggat tgtcgagatc gacgacgacg agagctttgt ccgtgaggat
    18901 gtaggcaaac gttccggcga tggtgatacg gcgagatccg gtgagggcgc cgttgggatt
    18961 aaaggtgacg gcgcgcttca ggaaattatt ggcgggattg ccatcgagga gcgtgccgac
    19021 gccgggactt ttcgatttga gattgggatc accaatgatg acgaggcctt cttctttgtc
    19081 ggcgacgtag aggaagccgt agagcaagtg gatcggctgt tcttcatttt ccggcaattg
    19141 ctgcctaagg ggatcgacac ccagcgtcgt tggtgaagcg acggcaagcg cgttcttcgt
    19201 gggcacataa aatttctggc cgatcggtga aaccggcgct gtcgtgactt tctcagaaaa
    19261 acttttgttg tcgatgttgg cgatgtcgta gacgcgcaga ccgccggggc cgagggcggc
    19321 gtaggcgtac tcgccgcgag cctgaatgtc gaggacattc cccggatgct cggcggcggc
    19381 gctgagttcg cgatgatgga ctagaaactt tttgtaattg ccgggatagg cgatgcgatg
    19441 gagggcgctg ccgtagacgg cttcgggttc gtcgtgctcg gcaacggcga tggcttcgaa
    19501 gccttttttg ccagtggcga catagacgta gcggcccatg aagttcatga agttggtgcc
    19561 ttgcaggagg agctgggcca tccaagcgtt gttatcgttc tgcgaggaga cgtggcagtc
    19621 gctgcactgt ttggtctctt tggcgcgtac ggtgtgaggg acaaaggtgg agaaagcctg
    19681 gccggagaag ccaggggcag atatggtttg ctgcgtgtaa taaagccaat cgcggttggc
    19741 attttgcgag ctgaccagca ctgcgcacga agaacgtgcg ggcgcgatgc gatggcctgt
    19801 gacggtgcca tcaacgccga gcatgtagat gtcgtcgcga agaacctgaa agttgtagct
    19861 agtgtaattc tttgtgacca ggccttcgtt gtggagcatg ggcttgcggg cgttagcagt
    19921 catctgcaga tggcagccga agcaagtggg cgtccacgaa gtgtggcagg agtagcaggt
    19981 catgctgctg ttggcgtgcg cgagagcggc atcgtcgctc ggcgtttgcg aagacacgac
    20041 gccatccttg ttcatgagct tcgcgcgaag ggatttctcg ctgaaatgca cattgccggg
    20101 cgtgatggtg tcgatggtct gaacgatctc ccactccttg ttctcttcgg acatcgagcg
    20161 ctggactacc ttgtcgccgc gccactcgaa tcggcgcaat ccccaagggg tccgaagcgc
    20221 gtcgagatga cggccgcctt ccggcgctgc cgggcctgac gtgaccagcg tggccttctt
    20281 ccgaatcgtg ccgtggcaat cgatgcagtc gatttcgaca gcggcacggg gctcgccgta
    20341 aattttgccg ttgccgtgat tgtcctgcgc gaagtggcaa tcattgcact gcatgccctt
    20401 ttcgagatga atatcggcaa gatgcacggc tttgccaaag cggtcgggat cttcaaaggc
    20461 gatctgc
//
LOCUS       DASYVO010000408         4412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_179021, whole genome shotgun sequence.
ACCESSION   DASYVO010000408 DASYVO010000000
VERSION     DASYVO010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179021"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..626)
                     /locus_tag="VGR03_13620"
     CDS             complement(126..626)
                     /locus_tag="VGR03_13620"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV1995363.1"
                     /translation="MSVIDEVLAANEIYSRTHEFRRLTPRPERKLAVLTCMDTRLSIR
                     TLGLREGDAHVIRNAGGIVTDDTLRSLVVSHYLLGTEEFMIINHTDCGLMHTTERDLR
                     TRIQNRAGTEAIAPAFFYAFQNIEENVRHQLQKLRTHPWIPKTVAVRGFVYDVTSGQL
                     REIKDS"
     gene            complement(738..1307)
                     /locus_tag="VGR03_13625"
     CDS             complement(738..1307)
                     /locus_tag="VGR03_13625"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV1995364.1"
                     /translation="MKKALLIVSTVVVLIGATVYADRATRVKPTRLVQSVDQANAKTI
                     GKPAPEVTFKDLDGKDATLDQYKGKVVLVNFWATWCDPCYIEIPWLIEMQQKYEAKGF
                     TVLGISMDEEGKSAVAPFLAKERFNVNGQKLPMNYPIVIGNDDVADKFGGLLGYPTSF
                     LVSRDGKIVKKVQGLISYEELTKAIESQL"
     gene            complement(1376..2278)
                     /locus_tag="VGR03_13630"
     CDS             complement(1376..2278)
                     /locus_tag="VGR03_13630"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEV1995365.1"
                     /translation="MNDVSLAATFLAGLVSFLSPCVLPLVPGYISMLSGIGMEQLRKG
                     EVPRSGLFASALAFVAGFSVVFISFGASASAVGSFLLQNRALLAPVAGALILLFGLHL
                     IGALIKLNLRTGIILGVILVALGIAAYFRHAPLFAGLGASHFFSLSLIGFFGPSMARW
                     LNRDVHLRSSATQPGIWSGFLLGFAFAFGWTPCIGPILSAVLILAARSDTIARGVLLL
                     AVYSAGLAIPFLLTALGISQFMVFYKNFRKYLHAVELFSGALLLLIGGLVFLNRLTWL
                     AAKLSFLNSLVLWLEHVLTGTGTK"
     gene            2706..3380
                     /locus_tag="VGR03_13635"
     CDS             2706..3380
                     /locus_tag="VGR03_13635"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEV1995366.1"
                     /translation="MRWTPNKVTFLRVVVGFAAVCLFGRGAWANLTAVGLTVTAIALD
                     ALDGHLARKKKMATPVGAQLDILGDRMIENVYFTYFAVVGMVSLWLPVLFFARGAATD
                     FLRGLAMKAGHSGWGANAMLRTWWGRALVASRWSRGLYAAMKCLCFCYLGLELALTRG
                     PVTLVGELAADVHAMIRVGAHVLTWMTAAFCLVRGLPVLVEGWKYIAGNVGPARGAKS
                     AAQEAM"
     gene            3377..4354
                     /locus_tag="VGR03_13640"
     CDS             3377..4354
                     /locus_tag="VGR03_13640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEV1995367.1"
                     /translation="MKERKEVGERKEFNEVREHEGNIAAFFDLDGTLVSLPSLERRLF
                     RTLRYRRAIPVKNYFLWLKEAMRLIPRGISAILQANKMYLRGVQILDERGEGDGDVSS
                     WHKDGHQAEGQASAPPRRNPRLPVPTFFAHAIETVAWHANQGHEIVLMSGTLEPLARG
                     AARVMEAELAARGITVTIRVFATRLEEMDGGWTGRVLGEAMFGKAKARAAKRLAEEML
                     LDLGRCYAYGDSLNDRWLMQTVGRPAAVNPSKDLAGIAQTRGWPVMRWSEEKTLTQRS
                     QSTQSSQRRTGRLGGGVDGEAEKQVNRRELKERLMNPDATGEAASRSLQ"
BASE COUNT         1000 a         1036 c         1527 g          849 t
ORIGIN      
        1 gatcgtctgc gtccttgtgg aatcgccata gtcccccaag atgcgggcac gggagcagtt
       61 acgtctcagc ttggaagacg ccgtccgacg tcccgtcgtc tcagaagccc aggatcgaac
      121 gagtttcaag aatctttgat ttcgcggagc tggccgctgg tcacgtcgta gacaaagccg
      181 cggacagcga cagtcttggg aatccaagga tgggtgcgga gcttctgcaa ttggtggcgc
      241 acattttctt cgatgttttg gaaggcgtag aagaaggcgg gagcgatggc ctcggttccg
      301 gcgcggttct ggattctagt gcgcaaatcg cgttcggtcg tgtgcatcag gccgcaatcg
      361 gtatggttga tgatcatgaa ttcctcggtg cccagcaagt aatgcgatac aaccagagag
      421 cgaagagtgt catcagtgac gatgccgccg gcgttgcgga tgacgtgggc atccccctct
      481 ctcagtccca gggtgcgaat cgaaaggcgg gtgtccatac aggtcaggac agcgagcttg
      541 cgctccggac gcggagtcaa tctgcggaat tcatgggtgc gcgagtagat ttcgttggcg
      601 gcgagaactt cgtcaatgac gctcatggcc agaagagtct agcaaaacag aagagagtga
      661 gcagtgaata gtgggtagtg gacaacaggg gccttctcgt tcttttcttc actacccact
      721 attcactgct cactctctta caactgagat tcgatggctt tggtaagttc ttcgtagctg
      781 atcagaccct gaactttttt cacgatcttc ccgtcgcggg agaccaggaa gctggtggga
      841 tagccgagaa ggccgccgaa cttgtcggcg acatcatcgt tgccgatgac gatgggatag
      901 ttcatgggga gtttctggcc gttgacgttg aagcgttcct tggcgaggaa gggcgcgaca
      961 gcggacttgc cttcctcgtc catggagatg ccgagaacgg taaagccttt cgcttcatac
     1021 ttctgctgca tttcgatgag ccagggaatt tcgatgtagc agggatcgca ccaggtggcc
     1081 cagaaattga cgagcacgac tttgcctttg tactggtcaa gcgtggcatc tttgccgtcc
     1141 aaatctttga aggtgacttc gggagcgggt ttaccaatgg ttttggcgtt ggcctggtcg
     1201 acgctctgca ccagacgggt gggtttgacg cgcgtggccc tgtctgcata gacagtggcc
     1261 ccgatgagca cgaccacggt ggagacgatc aagagagctt ttttcattta cgtcctcgaa
     1321 agttaattgt acctcgaagc gggtgctact tcccgcacgc aactggaagg gcgcgtcatt
     1381 tggttccggt accggtcagg acgtgttcca accataagac gaggctgttc agaaagctga
     1441 gcttggcggc gagccaagtc aggcgattca aaaagaccaa gccgccgatc agaagaagca
     1501 acgcgccgct gaagagttcg acggcgtgga gatattttct aaagttcttg tagaaaacca
     1561 tgaattgact gatgccgagc gcggtgagca ggaagggaat ggcgaggccg gcggagtaaa
     1621 cggcgaggag cagcacgcca cgggcgattg tgtcgctgcg agcggcaaga atgagcacgg
     1681 cactgaggat cgggccgatg cagggcgtcc agccgaaggc aaacgcgaag cctaggagga
     1741 agccgctcca gatgccgggt tgggtggcgc tgctgcggag gtggacgtcg cgattgagcc
     1801 agcgggccat cgaagggcca aaaaagccga tgagcgaaag cgagaaaaag tgagaagcgc
     1861 ccaggccagc aaagagcggg gcgtggcgga aataagcggc gatgccgaga gcgacgagga
     1921 tgacaccgag gatgatgcca gtgcggagat tgagtttgat aagcgcgccg atgaggtgaa
     1981 ggccgaagag gaggatcagg gctccggcga cgggagcgag gagggcgcgg ttctgcagaa
     2041 ggaaagagcc gacggcgctg gcggaggcgc cgaaagaaat aaaaacaaca gaaaagccgg
     2101 caacgaacgc gagcgcagaa gcgaagaggc cggacctggg aacctcgccc ttgcggagct
     2161 gctccatgcc gatgccggag agcatggaga tgtagccagg aacgagcggc agaacacagg
     2221 gcgagagaaa tgagacgagc ccggcgagaa atgttgctgc tagagaaaca tcgttcattc
     2281 tgattcagtc ctcggtagcg acttggaagc gcgacgaagc cgcgcatctg actagacttc
     2341 aagagaaact ttcttattct cccataaatc tcaatcttac tcgtgcttac gttatggtcc
     2401 tccccggcag ggccggaatc tacgatcatc gaccctcatt acttcgcttc agggtgacac
     2461 cacgaaggat gggtgtgcga gcggcaaaga cgaagccatt ttcttagacg cgcaagacca
     2521 ggaaaggaaa caaggcgggg aggtgcaaat aaacgacaga tgatggattt ggctggcgcg
     2581 aggtaacggt gactagttcg aggtgggctc taacgcattg atacaaaata agttaggtgc
     2641 cagggatttg gcagaggggt tgccatagcc tctgagagcg cccgggaggc gcgcggcaag
     2701 tgagtatgcg atggacgccg aacaaagtga cgtttctgcg agtcgtggtg gggttcgcgg
     2761 ctgtgtgctt gttcggtcgt ggggcgtggg ccaacctcac agcggtgggg ctgaccgtga
     2821 cggcgatcgc gctggatgcg ctcgacgggc atctcgcccg aaagaaaaaa atggcgaccc
     2881 cggtgggcgc gcaactggac attctgggcg accgcatgat cgagaacgtg tacttcacgt
     2941 atttcgcggt ggtggggatg gtgtcgctgt ggctgccggt tttgtttttt gcgcgtgggg
     3001 cggcgacgga tttcttgcgt gggctggcga tgaaagcggg gcattccggt tggggcgcga
     3061 atgccatgct gcggacctgg tgggggcggg cgctagtggc ctcgcgctgg agccgagggt
     3121 tgtatgccgc gatgaagtgc ttgtgcttct gctatttggg attggagttg gcgctgacac
     3181 gcggtccggt aacgctggtg ggtgaactgg cggcggatgt acacgccatg attcgcgtcg
     3241 gggctcatgt gttgacgtgg atgacggcgg cgttttgctt ggtgcgtggg ctgccggtgt
     3301 tggtggaagg gtggaaatac attgcgggaa acgtggggcc ggcgcgcgga gcaaagagcg
     3361 cagcgcagga ggcgatgtga aagagcgtaa ggaggttggg gagagaaagg aattcaatga
     3421 agtacgggag cacgaaggga acatcgcggc tttttttgat ttggatggaa cgctcgtgtc
     3481 gctgccatcg ctggaacgga ggctctttcg aacgttgcgc taccggagag cgatcccggt
     3541 aaaaaactat tttctgtggc tgaaggaagc gatgcgcctg ataccgcgcg ggatcagcgc
     3601 gatattgcag gccaacaaaa tgtatctgcg cggcgtgcaa attcttgacg agcgtggaga
     3661 gggagacgga gatgtctcct cttggcacaa agacggccac caggccgagg gacaggcgtc
     3721 ggctcctccg cggcgcaatc cacgattgcc tgtccccacc ttttttgcgc acgcgatcga
     3781 gacagtggca tggcacgcga accaggggca cgaaatcgtg ctgatgagcg ggacactcga
     3841 gccgttagcg cggggagccg cgcgcgtcat ggaagccgag cttgccgcgc gcggaatcac
     3901 ggtgacgatt cgcgtgtttg cgacacggct ggaagaaatg gacggcgggt ggacgggacg
     3961 ggttttgggc gaggccatgt ttgggaaagc gaaagcgcgc gcggcaaaga gactggctga
     4021 ggagatgcta ctcgacctgg ggcgatgcta cgcatacggc gacagcttga atgatcggtg
     4081 gttgatgcag accgttggcc ggccggcggc agtgaatcct tcgaaagacc ttgcgggcat
     4141 tgcgcagacg cgtgggtggc cggtgatgcg gtggagcgag gaaaaaactt taacgcagag
     4201 atcacagagc acgcagagtt cgcagagaag aacaggtagg ttggggggcg gcgtggatgg
     4261 cgaagcagaa aaacaggtga accgtcgaga actgaaagaa cgacttatga atccagacgc
     4321 gacgggagaa gcagcttcgc ggagtctgca atgaatagcg cggaaagaca gaatcataga
     4381 gtgaacgaag cggcgatcgt ctggcgcggg ga
//
LOCUS       DASYVO010000409         1761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_95091, whole genome shotgun sequence.
ACCESSION   DASYVO010000409 DASYVO010000000
VERSION     DASYVO010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95091"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VGR03_13645"
     CDS             complement(<1..571)
                     /locus_tag="VGR03_13645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1995368.1"
                     /translation="MSFLSGGVAVITGAGSGIGRALAQQLAEAGSALALADVDEAGLT
                     QTAESIGSKKALVTKNVVDVAEEASVSAFAEEVSSRHGRVTLLINNAGVALHGTFEEI
                     SLDDFRWLMNINFWGTVYGVKYFLPALKREERSHIVNISSVFGIIAPPGQPAYSASKF
                     AVRGFTEVLRHELADSAVCVSCVHPGGIRT"
     gene            complement(630..1322)
                     /locus_tag="VGR03_13650"
     CDS             complement(630..1322)
                     /locus_tag="VGR03_13650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1995369.1"
                     /translation="MTDSLPDKQLREEFNRWAEAGRGEGMEQDHLPITLPVLEKMRLA
                     PTANVLDVGCGAGWLSRRLARLVPEGRVIGMDISDEMIRHARRASVDFDNLMFVAGEV
                     AQIPWQPHFFSHAISVESSYYWPDPAAGIKDIFRVLQDGGSAWILINYYRDNPHCHQW
                     GDLLAVKTHLLLAHEWSELFRAAGFSMVKHERIVDRSPTPDVYTGRWFRDAEQMRAFK
                     AEGALLIHGAKE"
     gene            complement(1300..>1761)
                     /locus_tag="VGR03_13655"
     CDS             complement(1300..>1761)
                     /locus_tag="VGR03_13655"
                     /inference="COORDINATES: protein motif:HMM:NF019023.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1493 family protein"
                     /protein_id="HEV1995370.1"
                     /translation="ATDAKAEKLHLGTTLSGDLRMEGDDAVEFFEKFGSKFAVDLTDL
                     YRDWKFYFSHEGVPIATALLVAIPPVGIAILLERFFPRLHGMVALGIAALLWLPVLVQ
                     WSGWRYKNRTVQIAIEDLAQSASTGKWTKAVPEELVRRMNKPKFNDRFVAR"
BASE COUNT          370 a          571 c          463 g          357 t
ORIGIN      
        1 gcgtccggat gccgccggga tgaacgcacg agacgcacac cgcgctgtct gccagctcgt
       61 gacgcagcac ttccgtaaac ccccgcaccg caaacttgct tgccgaataa gcaggttgac
      121 ccgggggagc gatgattccg aagacactcg aaatgttaac gatgtgggat cgctcttccc
      181 tcttcagcgc gggcaggaaa tactttacgc cgtacactgt gccccaaaaa ttgatattca
      241 tcagccagcg aaaatcatcc agcgagatct cctcgaaggt gccgtggagc gcgacccccg
      301 cgttgttgat gagcagcgtt acgcgtccat gccgactgct gacctcttcg gcaaacgcgc
      361 tcacactggc ttcctcggcg acatccacca cgttcttcgt gaccaacgcc ttcttgcttc
      421 caatcgattc cgcggtctgc gtgagccccg cttcatccac gtcggcaagc gccagggcag
      481 aacccgcctc agccagttgc tgcgccagcg cacgcccgat gccggagccc gctccggtaa
      541 tcacggcgac tccaccactc aaaaatgaca ttggagaacc tcatggcgcg gcggcattcc
      601 tatcaccgaa ggccggaccc ggccacgttt cattctttcg cgccgtgaat gagcagcgcc
      661 ccttccgcct tgaacgcgcg catttgctcc gcgtcgcgaa accaccggcc cgtgtagaca
      721 tccggcgttg gcgaacgatc aacgatgcgc tcgtgtttca ccatggaaaa tcctgcggcc
      781 cggaaaagtt cgctccattc gtgagcgagg agtaggtgcg ttttcaccgc cagcaagtct
      841 ccccactggt ggcagtgcgg attgtcgcgg tagtagttga tcagaatcca cgctgagccg
      901 ccatcctgga gcacgcgaaa aatatctttg atgcctgcgg cagggtccgg ccagtaatac
      961 gaagattcca cggaaatcgc gtgcgagaaa aagtgcggct gccagggaat ttgcgcaact
     1021 tctcccgcga cgaacatcaa attgtcgaag tccacgctgg cgcgccgcgc gtgacggatc
     1081 atttcgtcgg aaatgtccat cccaataact cgcccctcgg gcactaatct cgccagccgc
     1141 cgcgaaagcc agcccgctcc acaacctacg tccagcacgt tggccgtagg ggcaagccgc
     1201 atcttctcca gcaccggcag cgtgatgggc aggtgatcct gctccatccc ttcgccccgc
     1261 cccgcctccg cccagcggtt aaactcctcg cgaagttgct tatcgggcaa cgaatcggtc
     1321 attgaatttc ggtttgttca ttcggcgtac aagttcttcg ggcacagcct ttgtccattt
     1381 tccagtggaa gcgctctgtg ccaagtcctc aatcgcgatc tggaccgtcc gattcttgta
     1441 ccgccacccg ctccattgca cgagtaccgg cagccagagc agagcagcaa ttccgagagc
     1501 aaccattccg tgcagccgtg ggaaaaatct ttccagaagg atcgcaatcc caacaggcgg
     1561 aattgcgact aggagggctg tggcgatggg taccccctca tgggagaagt agaatttcca
     1621 atcccgataa aggtctgtca gatccacggc gaatttgcta ccgaatttct caaagaactc
     1681 gactgcgtcg tccccttcca tgcggaggtc cccactcagc gttgtaccca gatgtagttt
     1741 ttccgccttc gcatcggtgg c
//
LOCUS       DASYVO010000410        13604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_249546, whole genome shotgun sequence.
ACCESSION   DASYVO010000410 DASYVO010000000
VERSION     DASYVO010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 13604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13604
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249546"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="VGR03_13660"
     CDS             complement(<1..1303)
                     /locus_tag="VGR03_13660"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEV1995371.1"
                     /translation="MAMSFGDLWRWDGKVGRGKYAAVGLIGVVIKHNLDRLIAGSFLG
                     YEKSFFNYWAPLGKAARLDHLSSTEVNFLATILLMSIPFLWVGVAMTVRRLRDAGQPV
                     WLVVLFFIPVINVLFFLALCALPPRERAEDSEGAPWPGPSGLDGIISSSPLGSAVLSI
                     FLTSVLGLVFLALGTLVIGAYGWGLFVALPFCLGMFSVLLYSYHAPRDWQSCLKVALL
                     PVLVLGVAFILVAMEGLICVLMAAPFALGLAALGGMLGYTIQAHHWRSKQTPAMLSLV
                     ILMIPASFGIEHAAALQPPVFMVRTAIEVNAPPEKVWNQVVAFAEIPAPKELLFRAGI
                     AYPIRAEISGRGVGAVRHCVFSTGPFVEPIEVWDEPRLLQFGVTANPAPLNEISPYGN
                     IQPPHLHGYFVSEKGQFSLTALPGGRTWLEGTTWYQHTMWPA"
     gene            complement(1341..1760)
                     /locus_tag="VGR03_13665"
     CDS             complement(1341..1760)
                     /locus_tag="VGR03_13665"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEV1995372.1"
                     /translation="MISLVSEYTDGKGRHARGWLFFDAECKFCTRTARWIAPILERRG
                     MALAPLQDPRVGALLGMPREELMLEMRFLLSDGSQYGGADAAVALAREIWWWRPLLWA
                     SRIPGMMEILRKGYRRIAMSRSCAAVSCPANESSTRV"
     gene            complement(1868..2425)
                     /locus_tag="VGR03_13670"
     CDS             complement(1868..2425)
                     /locus_tag="VGR03_13670"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HEV1995373.1"
                     /translation="MSEPTKNSLTPVAQKFILHWGEMGTRWGINRTVAQVHALLFLSP
                     QPLPADEISATLAVARSNVSTSLRELQGWRIVRVVHVLGDRRDHFESTKDVWEIFRIV
                     SEERKRREIDPTLSVLAECVREVKSNPQGDAYTRERLESMLKFLTAMTGLFEEIIRMP
                     TVALKGVVKLRGKVITLLGKKAVNS"
     gene            2582..2917
                     /locus_tag="VGR03_13675"
     CDS             2582..2917
                     /locus_tag="VGR03_13675"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV1995374.1"
                     /translation="MTSQNFRHLALSFPETEERAHMDHPDFRVAGKIFATLGYPEDGW
                     AMVKLTPIEQEMFIKAHPTIFKPCNGAWGRRGATNVRLKAAKKPTVRRALEAAWRLAA
                     PKTLTRPPI"
     gene            3018..5444
                     /locus_tag="VGR03_13680"
     CDS             3018..5444
                     /locus_tag="VGR03_13680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1995375.1"
                     /translation="MANFARNVRFGLRLLGKNLGFTCVALLALILGIGANTAIFSVVH
                     ATLLSPMPYPNPDQLVMVWSKINGDRNGVSAGDFLDWKRQSTVFQSICAWSGGSYTLS
                     ASDHPEQLQTRVVTPGFNDMVGTPYFLGRDFLPEEGEVGRDRVVVMTHRLWEERFGSD
                     RDIIGKPVRLNGERYAVVGVLAAGQPDRLESQLFVPLAFKPEQINHDFHFILVMGRLK
                     PGVTLQQANADMDSVTRHIAEVYPKSNKGWGATVEPLQNDFISKDTIKNLWLLMGAVG
                     FILLIACVNVANLLLARGTVRQKEIAVRAALGATRWQLFAQFLTESVALSLIGGALGV
                     GLAWIMLKVIVAIMPPFTLPSEADLRLNLPVLFFSFAASLVSGVLFGCAPAWQTARQN
                     LSDTLKETGRSAVGAGRHGLRRTLVVIEFALALTLLAGAGLVIHSFWKLSRVELGFRQ
                     DHILTFFLPVTNERFAHPEQINAFYRQLLDKIGALPGITAVTASTGMPIIGTNFGMPF
                     NLAGQAVEDPSSRPGAGFNMVTPEYFRTFGIRMIQGRTLTEQDAAGGLPVAVVNETFA
                     KKYLSKVDPLTQRIVVDQLIPGVTKLGPAVEWQIVGVYHDVHNGGVRGDGFPEIDVPF
                     WQSPWPQAGLAARTFGDPASMTKSIAAVVRSVDPDLPLDQVKTMDQLVHESLANDRFS
                     TALFGSFAGVALLLAAIGIYGVMSFAVAQRTHEIGLRIALGAGPRQVLRLVLQEGMLL
                     AFAGLVVGLAGTYSVGRLMKSVLYQVNAMDPAAISAVTAVLLLSALLACYIPARRATQ
                     VDPMVALRDE"
     gene            complement(6088..7410)
                     /locus_tag="VGR03_13685"
     CDS             complement(6088..7410)
                     /locus_tag="VGR03_13685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV1995376.1"
                     /translation="METVPPIRDLLFTLLLKVGVASSIAALLARWNTFRRVLFTEERD
                     LDQKLKLMLFMTPALIVGVTLRLVGGPPYSFADLSLEGAFLMGLLGGRVVGPIGGAII
                     TIPALISHEWLAMPAASTAGLLGGLIRQAIPNKEDLWNFGPFTFLNIPKATMRLLRKA
                     ELSWEMAPLGACVGLELGRVALVLATKPKWLFAIDAKWDGWLVLVVIATLMSVAAPLK
                     IWNNTRIEMNLERHQQLLLKARMDALSSQINPHFLFNTLNTVSALIRFDPDSARGVVL
                     KLSNILRRLLRKHETFVPLREELQFIDDYLDIEVARFGKDNLDIVKHIDDAALEAFVP
                     SMLLQPMVENCLKHGLAPKLGGGKIELRTTSRDGRLRIEIADNGVGISEEKMPHVYVE
                     GIGLSNVRERLRVLYGTDFHLDIQSRPGEGTVIRIEIPELVSTMQEVR"
     gene            7607..8215
                     /gene="ribA"
                     /locus_tag="VGR03_13690"
     CDS             7607..8215
                     /gene="ribA"
                     /locus_tag="VGR03_13690"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEV1995377.1"
                     /translation="MNRRVTALRMVAHAALPTRYGRFTICGFKGRGPQEEAVALVRGN
                     LSGKTAPLVRVHSQCLTGDVLASLRCDCRAQLELSLKKIGQASSGILLYLPQEGRGIG
                     LMNKLRAYELQDGGMDTVEANETLGFAADARDYEFSAQILKKLGATRIRLLSNNPEKV
                     RQLESFGIHVAERLPCQPRISKLSRDYLKTKKRKMGHLLEGI"
     gene            complement(8233..8445)
                     /gene="thiS"
                     /locus_tag="VGR03_13695"
     CDS             complement(8233..8445)
                     /gene="thiS"
                     /locus_tag="VGR03_13695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEV1995378.1"
                     /translation="MQTTISILVNGEDRQATPGATVTDLLREMGLDSGRVAIERNLEI
                     LPRPKWTETTVEAGDRYEIVQFVGGG"
     gene            complement(8449..9078)
                     /locus_tag="VGR03_13700"
     CDS             complement(8449..9078)
                     /locus_tag="VGR03_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995379.1"
                     /translation="MLFGHNTNVKAGDTTYHVQTEDRGTTNALIDTTVYFRGRVLHRR
                     TNNYFDLLPPNPDSEQALKLRLDEQHRAVVEEIRTGALHLALPRDEKSASQTPAAAPA
                     QRSQVLLLELINAKTWLSGKRALLQVAVRNEAHQAVGGATVSARVDGAAEVTDFSAQT
                     GDFGHAQFEFDMPRLAGAEPALVIEALHGNARGHLRFHLRTKPHVPSAS"
     gene            9184..9555
                     /locus_tag="VGR03_13705"
     CDS             9184..9555
                     /locus_tag="VGR03_13705"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEV1995380.1"
                     /translation="MSGSGLHTYAPLLLHLLIAAALAGALTAVSVLVGWRRPSRAKQQ
                     PYECGMEPTGDARAPFSVKFYLVAMVFILFDVEAIFLYPWAYIFRSLRWYGFGEMMLY
                     IAILLVGYLYLWKKGALDWHK"
     gene            9652..10104
                     /locus_tag="VGR03_13710"
     CDS             9652..10104
                     /locus_tag="VGR03_13710"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEV1995381.1"
                     /translation="MEVIEFRGETTIVVPRELLRATAEHCRGDAKLQYNFLTDATCVD
                     RYPSEPRFELNYHLVSIPRREKVRLRVWLGGNDPVVDSLVPVWPGANWLEREIFDLFG
                     IRFAGHPDLRRILLPDDWEGHPLRRDYPVEGYRDVPNTGELFKKSGTP"
     gene            10101..11297
                     /gene="nuoD"
                     /locus_tag="VGR03_13715"
     CDS             10101..11297
                     /gene="nuoD"
                     /locus_tag="VGR03_13715"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEV1995382.1"
                     /translation="MTTVETAVGTAKTMVLNMGPQHPSTHGVLRILLELDGETVVKAV
                     PDLGYLHTGIEKSCEDKTYSQVITLTDRMDYLNPLGNNLVYCLAVEKLLGLEVPKRAQ
                     YIRVMMVELQRISSHLVWLGTHAIDLGAMTVFLYCFREREEILKIFELFSGQRMMTSY
                     IRIGGLALDPPTGWRQAVERFLKMMPSRVDEYETLLTENRIWIGRTKGVGFISAEDAI
                     ALSMSGPTLRAAGVAYDNRKLFPYSSYEEFDFGVPTRTESDCYARYLVRVAEMRESLK
                     IIRQTLEKISNEGPIMADAPGIVPPQREKMKTEMEALIYHFKIFTEGFSPAPGEVFVT
                     VESPRGELGCFVASDGSPKPLRVHFRSPSYINLQALPKLIEGQLISDVIACIGTIDIV
                     LGEVDR"
     gene            11300..13114
                     /gene="nuoF"
                     /locus_tag="VGR03_13720"
     CDS             11300..13114
                     /gene="nuoF"
                     /locus_tag="VGR03_13720"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEV1995383.1"
                     /translation="MELSPTLSAKFEKLQSSYPVKRSALIPMLMYAQDEYGYISDEMI
                     AELGRRLDLNNVQVEETLAYYSMLHRKPWGKNHVQICTNVACMLKGGYSMLDRAKKRL
                     EIGHKETTQDGVFSLEEVECIGACTGAPAMQVNYDFFENLTPLKFDRIIEDLDAGRRP
                     TPEPVISGAIHERHPAETPLISRRWGMKDSHKIEVYLMQGGYQALEKALKEMTPESVI
                     DEVKKSNLRGRGGAGFPTGMKWSFVPKDSPKPKYVICNADESEPGTCKDRPLMEMDPH
                     QLIEGMVIAGRAIGSHQGFIYIRGEYRYVLNIMDAAIAEAYAHGYLGKNIAGSGFDFD
                     LLAHTGAGAYECGEESALMESLEGKRGYPRIKPPFPAVVGLYGCPTIINNVETLSAVP
                     SIIREGANAYANRGTPKNGGTRMLCIAGHINKPGIYEVPLGMNMKKFIYEVAGGIPRG
                     KKLKAVIPGGSSCPLLTADEIDIAMDYDSVAKAGSMLGSGGMVIMDEDTCMVDMARRI
                     MHFYAHESCGWCIPCREGTIWLRKILERFHAGLGRAEDIDMIAELAKNMLGRTFCPLG
                     DAAAMPTISIMKKWRGEFEEHLKGRCASKSAEALVGSR"
     gene            13121..>13604
                     /locus_tag="VGR03_13725"
     CDS             13121..>13604
                     /locus_tag="VGR03_13725"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEV1995384.1"
                     /translation="MAEQKLIKLKINEREVQVPQGTLVIEATRRIGTEVPSFCYYPGL
                     SLQAACRMCLVEVEKAPKLQTACTLMATEGMIVRTDTEQVRQARKYMLEFLLTNHPLD
                     CPVCDKGGECDLQDFSFYHGAGKSRFIEEKLKYPKPVPLSDKILLDMERCVLCERCVR
                     Y"
BASE COUNT         2832 a         4192 c         3806 g         2774 t
ORIGIN      
        1 ccgccggcca catcgtgtgc tgataccaag tcgttccttc gagccacgtc cgcccgccgg
       61 gcaacgccgt caacgaaaac tggcctttct cggaaacgaa gtagccgtgc aaatgcggcg
      121 gctgaatatt tccatacgga ctaatttcat ttagcggcgc cggattcgcg gtcaccccaa
      181 actgcagcag ccgcggctcg tcccaaactt cgatcggctc cacgaacggc cccgtcgaga
      241 aaacgcaatg tcgcaccgcg ccaactccgc gcccggaaat ctccgcgcga atcggatatg
      301 caatccctgc ccgaaacaac aattccttcg gcgcggggat ctccgcaaac gccaccacct
      361 gattccaaac tttctccggc ggcgcattca cctcaatcgc cgtccgcacc atgaacacgg
      421 gtggctgcag cgccgcggca tgctcgatcc caaacgacgc tggaatcatc aagatcacga
      481 gcgacagcat cgccggcgtt tgcttcgacc gccagtgatg cgcctgaatc gtgtagccca
      541 acattccacc caacgccgcc agccccagcg caaaaggcgc tgccatcaac acgcaaatta
      601 ggccttccat ggccaccaaa ataaatgcca cacccagcac caacacggga agcagcgcaa
      661 cctttaagca agactgccaa tcccgcggcg cgtggtagct gtagagcagc accgaaaaca
      721 ttcccaaaca aaacgggagc gccacaaata gtccccagcc ataagccccg atcaccagcg
      781 ttcccagcgc cagaaacacc agccccaaaa cagacgtgag aaatatcgaa agcacggcac
      841 tgccaagcgg actgctcgaa attattccgt cgagtccact cggtccaggc cacggcgcgc
      901 cttcggaatc ctcggcgcgc tctcgcggcg gcagcgcgca aagcgccagg aaaaacagca
      961 cgttaattac aggaataaag aaaagcacca ccagccacac cggctgcccc gcatctcgca
     1021 accgccgcac ggtcatcgcc accccgaccc acagaaacgg gatcgacatc aagagaatcg
     1081 tggcgagaaa attcacttcg gtactcgaga ggtggtcgag cctcgccgct ttccccagcg
     1141 gcgcccagta gttgaaaaaa cttttctcgt aacccaaaaa tgagccagcg atcagccggt
     1201 caagattgtg cttgatcacc acaccgatga gacccaccgc cgcatatttc ccacggccaa
     1261 cttttccgtc ccatcgccac agatcaccaa agctcatcgc catcgccggc tctccttgat
     1321 gcagcgcctg caactcgttc ctaaactcgc gtcgaagact cattcgccgg acaagaaacc
     1381 gccgcgcaac tccggctcat cgctatccgc cgatatcctt tgcgcaatat ttccatcatc
     1441 ccgggaatcc tcgacgccca gagcagtggt ctccaccacc agatttcgcg cgccagcgcc
     1501 accgctgcat ccgccccgcc gtactggctc ccatcgctaa gcagaaatct catttcgagc
     1561 attagttctt cccggggcat tcccagcagt gcaccgacgc gcgggtcctg cagcggtgcc
     1621 agcgccatcc cgcgccgctc cagaatcggc gcaatccacc tcgcagttcg cgtgcagaac
     1681 ttgcactccg cgtcaaagaa caaccacccc cgcgcgtgcc gccccttccc gtccgtgtac
     1741 tcactcacca gcgaaatcat ggttccttct cttccttact ttcgtgcttc tccgcgtgcc
     1801 cctgcgttct ctgtgctctc tgtgttaggt ctcttccgct ttttccgaaa ctttcaactc
     1861 taaactgtca actgttgact gcctttttcc ccaacagcgt aatcaccttc cctcgcaact
     1921 tcaccacgcc cttcagcgcc accgtcggca tccggatgat ctcttcaaac aatcccgtca
     1981 tcgccgtcaa aaacttcagc atgctctcca gccgttcccg cgtgtacgca tcgccctgcg
     2041 gattcgactt gacctcccgc acgcactccg ccagcacact cagcgtcgga tcgatctcac
     2101 gccgcttccg ctcttccgaa acgattcgga agatttccca cacgtccttg gtcgattcaa
     2161 aatggtcccg ccggtccccc agcacatgca ccacgcgcac gattcgccat ccttgcagct
     2221 cgcgcaggct ggtgctcacg ttcgaacgcg ccaccgccag cgtcgcggaa atctcatccg
     2281 ccggcagcgg ctgcggtgac aggaacagca gcgcatgaac ctgcgctacc gtccggttga
     2341 tgccccaccg ggtccccatc tctccccaat gcaaaatgaa cttctgggcc accggggtta
     2401 agctgttttt tgtcggttca ctcatttttg cctttccgtc cctggcgtcc ttcctgccgt
     2461 attcgttatg tcggttgttt ctgttgttac agaatataca gttgtttcct ctccttgtca
     2521 agctctttct tctccgcccc gctcgtgctc ccatggctct ctcaacaaac cagagccacc
     2581 catgacctct caaaactttc gccacctcgc cctcagcttc cctgagacgg aggaacgcgc
     2641 ccacatggac catcccgatt ttcgcgtcgc cggaaaaatc ttcgccaccc tcggctaccc
     2701 cgaggatggc tgggccatgg tcaagctcac gcccatcgaa caggagatgt tcatcaaagc
     2761 ccatcccacc atcttcaaac cctgcaacgg tgcttgggga cgccgcggcg ccaccaacgt
     2821 ccgcctgaag gccgccaaaa agcctactgt gcgccgtgct ctcgaagcgg cctggcgcct
     2881 cgccgccccc aagacgctga cccgcccgcc aatttgacga aaatcgataa aactgccctt
     2941 gcggccgtga ccacttggcc gaccgaacgt ctaatcacct caacgggtgg tcatctggcg
     3001 agcttggggg aaccagcatg gcaaattttg cgcgcaacgt tcgtttcggc ctccgattgc
     3061 taggcaagaa cctcggcttc acgtgcgttg ctctactggc gctcattctg ggtatcggcg
     3121 caaacaccgc cattttcagc gtcgtccacg ccacgcttct ttctcccatg ccatacccaa
     3181 atccggacca gttggtgatg gtctggtcga aaatcaatgg agaccgcaac ggagtctcag
     3241 cgggcgactt tctggattgg aagcgccaga gcaccgtctt tcagtccatt tgcgcctgga
     3301 gcggcggcag ttacacgctt tccgcgtccg atcatcctga acaactccag accagggtag
     3361 tcacgccggg cttcaacgac atggtgggta ctccatactt tctagggcgg gattttctgc
     3421 cggaggaagg ggaggtcggc agagaccgcg tcgtcgtgat gacgcaccgg ctctgggaag
     3481 agcgattcgg cagcgaccgc gacatcatcg gcaagccggt ccgcctgaat ggcgagcgct
     3541 acgcggtcgt tggagtgttg gccgccggcc agcccgaccg cctggaatcg cagctcttcg
     3601 tgccactggc tttcaaaccg gaacagatca accacgattt tcattttatt ttggtgatgg
     3661 gccgcctgaa accgggagtc actctccagc aggctaacgc ggatatggac tccgtcaccc
     3721 gccacatcgc ggaagtctat ccaaaatcaa ataaaggatg gggtgccacg gtcgaaccct
     3781 tgcaaaacga tttcatcagc aaggacacca tcaaaaatct ctggctgctc atgggcgctg
     3841 tcgggttcat cttgttgatt gcttgcgtga acgtggccaa tctacttctg gcgcggggca
     3901 ccgttcgcca aaaggaaatt gccgtgcggg ccgcgctggg cgcaacacgc tggcaactct
     3961 tcgcgcaatt cttgacggag agcgtcgctc tgtctttgat tggcggagcc ttgggagtgg
     4021 gtcttgcctg gatcatgctg aaagtgattg tagccatcat gccgcctttt actctgccct
     4081 ccgaagccga cctgcgattg aatctgcccg tactcttctt cagctttgcg gcatcactgg
     4141 tgtccggcgt tctgtttggc tgtgctccgg catggcaaac cgcgcggcag aatctgagtg
     4201 acacgttgaa ggaaaccggg cgctccgcgg tcggcgccgg ccgccacgga ctgcgccgga
     4261 cgcttgtggt catcgaattt gccctggctc tcacgcttct cgcgggcgcc gggctggtga
     4321 ttcacagttt ctggaaattg agtcgcgtcg aactcgggtt tcgccaggac cacatcctca
     4381 cgttttttct tcccgttacc aacgagcgct tcgctcatcc cgaacaaatc aatgcttttt
     4441 atcgccaact cttggataag atcggcgccc ttccaggaat caccgccgtc accgcctcca
     4501 ccggcatgcc aatcatcggc acaaacttcg gcatgccctt caaccttgcg ggacaggctg
     4561 tcgaagaccc gtcctcgcgg cctggcgctg gctttaatat ggtcactccg gaatacttcc
     4621 ggacctttgg catccgcatg attcagggcc gcacgttgac cgaacaagac gcggcgggcg
     4681 gcctgccagt cgccgtcgtc aatgaaacct ttgccaagaa atacttgtcc aaggttgacc
     4741 ccttgaccca gcgcattgtt gtggatcagt tgattcccgg tgtcaccaaa ttaggccccg
     4801 cagtcgaatg gcaaatagtg ggcgtctacc atgacgtcca caacggtggt gtccgcggcg
     4861 acggcttccc cgaaatcgac gttcccttct ggcaaagtcc ttggccacag gcgggcttgg
     4921 cggcgcgcac ctttggcgat cccgccagca tgacgaagag cattgcggct gttgttcgct
     4981 ccgtggaccc cgatcttcct ctcgaccagg taaaaaccat ggatcaactc gtccacgagt
     5041 ccctggccaa cgatcgtttt tctacggccc tcttcggcag ctttgccggc gtcgcgctgc
     5101 tcctggcggc aatcggcatt tacggtgtga tgtcgtttgc cgtggcccag cgcacccatg
     5161 aaatcggact gcgcatagcc ctcggcgcgg gcccccggca ggtgcttcgt ctggtgcttc
     5221 aggaggggat gcttctcgcg tttgcggggc tagtagtagg tctggctggc acgtactctg
     5281 tgggccgcct aatgaaatcc gtcttgtatc aggtgaatgc tatggatccc gcggccatca
     5341 gcgccgtcac cgcggtcctc ttgctctccg cgttgctggc ctgctacatc cccgcgcgcc
     5401 gcgctaccca agtcgatccc atggtcgcgc tgcgcgacga atgagcttcg cctgaactgc
     5461 atccgggagt gaaccgcgtc ctcggcgggc ggcaatagga gaaatattca gcccggactt
     5521 ctcacacgaa tctggtctgc ccacgcgctt catccaaaat ccagcagagc gctggtgaga
     5581 aatccacgct aaggattcgc tatgaggacc gggccttatt tcctcgccat tccttcgccg
     5641 aaagaaagcg catgcacggg cagaagcctt tatggtcgcc cggggcctga caggaatact
     5701 tcagcgccgc gtggctgccg gctcgatgcc cgcacgtgca catctcccgt gcaagctcag
     5761 gcgaaactcc cgctgggcgg cctcgtacac cgatcagcgg ttttgcgtct tgcaaattct
     5821 tactagtcac tgttcctctc tctttgcgta ccctgggtgc cgttctggca cgccagaggc
     5881 ctcgcacctc ttagtttcgc attctcactg gcgaatatca aagcgtccaa gccctgcggg
     5941 aagctcagat ttcccggacc gctctctgag agggatgaag ctacttctta gtttgcgcct
     6001 ctgtccctgt aactacaact cagtccagcc gacaatttcg atggcaaatc gccgactggc
     6061 cgactccagt ttgaattttt agcctggtca tctcacttct tgcatcgtcg ataccagctc
     6121 cggaatctcg atgcggatca ccgtgccctc acccggccgg ctctggatat ccaaatgaaa
     6181 atccgttccg tacaaaacgc gcagccgttc gcggacgttg ctcagcccga tgccctcaac
     6241 gtaaacgtgc ggcatcttct cttccgagat gcccacgcca ttgtctgcaa tctcgatgcg
     6301 cagccgaccg tcgcggctcg tcgttctcag ttcgattttt ccgccgccca gcttcggcgc
     6361 cagtccatgc ttcaggcaat tctccaccat cggctggagc agcatgctgg gcacaaacgc
     6421 ttccaacgcc gcatcgtcaa tgtgcttcac gatatccaga ttgtcttttc cgaatcgcgc
     6481 cacctcgatg tccagatagt cgtcgatgaa ttgcaactcc tcgcgaagcg gcacaaacgt
     6541 ttcatgcttg cgcagcaacc gccgcagaat gttgctcagc ttcaggacca cgcctcgagc
     6601 cgaatccgga tcgaaccgga tcagtgccga cacggtgttc aaggtgttaa acagaaaatg
     6661 cggattgatc tggctggaga gcgcatccat ccgcgctttc aacagcagtt gctggtgccg
     6721 ctccagattc atctcgatgc gcgtgttgtt ccaaatcttc agcggcgccg ccaccgacat
     6781 cagcgtcgcg atcaccacca gcaccagcca cccatcccac ttggcgtcga tggcaaacag
     6841 ccacttcggc ttggtcgcca gcaccagcgc cacgcgcccc agttccaggc ccacgcacgc
     6901 ccccaatggc gccatttccc acgacaattc cgctttccgc aacaggcgca tcgtcgcttt
     6961 cggaatgttc aaaaacgtga acggcccaaa gttccagaga tcttctttat tgggaatcgc
     7021 ctgccggatc aatccgccga gcaaccccgc ggtggatgcc gccggcattg ccagccattc
     7081 gtgcgagatc agcgccggaa tcgtgatgat cgcgccgccg atcggtccca cgacgcgccc
     7141 gccgagaagg cccatcagaa aagcgccttc taacgacaaa tccgcaaacg agtacggagg
     7201 ccctcccacg agccgcagag tgacgcccac gatcagcgcc ggcgtcatga acagcatcag
     7261 tttgagtttt tggtcgagat cgcgttcctc ggtgaagagc acgcgccgaa acgtgttcca
     7321 ccgcgccagc aacgcggcaa tggacgacgc cacccccacc ttcagcagca gggtgaacag
     7381 cagatctcga atgggtggca ctgtttccat tttggggacg agtctcaggt taacgctaat
     7441 caatccgccg ctcgatgtaa agccggtagc atccgcagac gccccacgca gcaaatggcg
     7501 ggcatcgaat caacccttgg caaagcgagc tgaaatcggt gcgaaacaca gtccctgcta
     7561 tacttctcgt ttcccgatga gcgcaaaatc gcagcaaggc aaaaaattga atcgaagagt
     7621 caccgccctg cggatggtcg cgcatgccgc gcttcccacg cgttatggcc gattcaccat
     7681 ctgcggattc aagggccgcg gccctcagga agaagcggtc gcgttggtcc gcggaaactt
     7741 gagcggaaag accgctccgc tggtgcgcgt ccactcgcag tgcctcaccg gcgacgttct
     7801 tgcctcgctt cggtgcgact gccgcgcgca actcgaactg tcgctgaaaa aaataggcca
     7861 ggcatcctcg ggaattctgc tgtacctgcc ccaggaaggc cgtggcatcg gcctgatgaa
     7921 caaactgcgg gcttacgaac tgcaggacgg cggaatggac acggtggaag caaacgaaac
     7981 tctggggttc gcggccgacg cgcgcgatta cgaattttcc gcgcaaattc tgaaaaaact
     8041 tggcgcaacg agaattcgtt tgctttccaa caatccggaa aaagttcgcc aactcgaaag
     8101 ctttggcatt cacgtcgccg agcgcctgcc gtgccagcct cgcatttcaa aactttcgcg
     8161 cgactacctc aagacgaaaa agcgcaaaat ggggcatctg ctggaaggaa tctgagattt
     8221 gaagatatgc tgctacccgc cgccgacaaa ttgcacgatt tcgtagcggt ctccggcttc
     8281 gactgtagtt tcggtccact tcggccgggg caaaatttcc aggttccgtt caatggccac
     8341 gcgtcccgaa tcgagtccca tctcgcgaag caaatccgtc actgtggcgc cgggcgtcgc
     8401 ctggcgatct tctccgttaa ccaaaatcga aatggttgtc tgcatgtgct aactcgccga
     8461 cggcacgtgc ggcttggtgc gcaggtgaaa acgcaagtgc cccctggcat ttccatgcaa
     8521 cgcttcgatg accagcgccg gctcggcacc cgccagccgc ggcatatcga attcgaattg
     8581 ggcatggccg aagtcgccgg tttgcgcgga aaaatctgtc acctccgcag cgccatccac
     8641 gcgggcgctc acggttgcgc cgcccaccgc ttggtgagct tcatttcgaa cggcgacctg
     8701 cagcaaggcg cgtttgccgg agagccaggt cttggcattg atcagctcga ggagaagaac
     8761 ttgcgaccgt tgagcggggg ccgctgcggg cgtttgcgat gcagattttt cgtcccgtgg
     8821 cagcgctaga tgcagcgcgc ctgttcgaat ctcctcgaca acggcgcggt gctgctcgtc
     8881 gagacgcagc ttcaaggctt gttcgctgtc cgggttcggc gggagcaagt caaagtaatt
     8941 gttcgttcgg cgatgcaaca ctcgtccgcg aaagtagacg gtggtatcga tgagcgcgtt
     9001 ggtggtgccg cgatcttccg tttggacgtg atacgtcgtg tcgcctgctt tcacgttggt
     9061 gttgtgaccg aagagcattt ccgagtttgc cccgacctgc ccaagggctg aacgcgcccc
     9121 gcaggaatct ttctgccgca tttgaccgac tggcggttga atcgttatta tagcgcttcg
     9181 cctatgagtg gttctggttt acatacgtat gctccgcttc tcttacacct gctgattgcc
     9241 gcggcgctcg cgggcgcgtt gaccgctgtt tctgttctgg tgggctggcg gcgtccgagc
     9301 cgggccaagc agcagccgta cgagtgcggc atggaaccca caggcgacgc gcgcgcgccc
     9361 ttctcggtca agttttacct cgtagcgatg gttttcattc tgttcgacgt ggaagccatc
     9421 ttcctgtacc cctgggctta catttttcgc agtttgcggt ggtacggctt tggggagatg
     9481 atgctctaca ttgccatcct gttggtcggt tatctgtatc tctggaaaaa aggcgcgctg
     9541 gactggcata aatagccggc gcatgtggga tccgaaatcg tgacggagtc ttccagtaaa
     9601 aacgctaatg ttgtagccga atgcctgcgt tcgtggaacg cgaaggcagt tgtggaagtg
     9661 attgagttcc ggggcgagac cacgatcgtc gtgccgcgcg aactcctccg cgcgacggca
     9721 gaacactgcc gcggagacgc aaagctgcag tacaacttct tgacagacgc cacctgcgtg
     9781 gaccgctatc cgtctgagcc gcgatttgaa ttgaactatc atctggtttc catcccgcgc
     9841 cgtgaaaaag tgcgattgcg agtatggctc ggcggcaacg atccggttgt cgattcgctg
     9901 gtgccggtct ggccgggcgc caactggctg gagcgggaaa tttttgacct ctttgggatt
     9961 cgctttgccg ggcatcccga cctgcggcgc attctgcttc ccgacgattg ggaaggccat
    10021 cccttgcgca gagattatcc cgtggagggc tatcgagacg tgccgaacac gggagaacta
    10081 tttaaaaaga gcggcacacc atgacaaccg ttgaaaccgc tgtcggaaca gcaaagacca
    10141 tggtcctcaa catgggaccg cagcacccct cgacgcacgg ggtactgcgc attttgctgg
    10201 agctcgacgg cgaaacggtg gtgaaagcgg ttcccgatct tggctacctg cacacgggaa
    10261 ttgaaaagtc ctgcgaggac aaaacctatt ctcaggtcat cacgctcacg gatcggatgg
    10321 attatctgaa tccgctgggg aacaacctgg tgtactgcct ggcggtggaa aaactgctcg
    10381 ggttggaagt gccgaagcgc gcgcaataca tccgggtaat gatggtcgag ctgcagcgga
    10441 tttcttcgca tctggtgtgg ctcgggacgc atgcgatcga cctcggggcg atgaccgttt
    10501 ttctttactg cttccgggag cgcgaagaga tattgaagat tttcgaattg ttctccgggc
    10561 aacggatgat gacgagttac attcgaatcg ggggcctggc gctggatccg cctaccggtt
    10621 ggcgacaggc ggtcgagcgt ttcctgaaga tgatgcccag ccgcgtggac gaatacgaga
    10681 cgctgcttac ggaaaaccgg atctggatcg gccgcacgaa gggcgtcggc ttcatatccg
    10741 cagaagacgc cattgcactg agcatgtcgg ggccgacgct gcgcgccgcg ggcgtggcgt
    10801 acgacaatcg aaagctcttt ccctactcga gctatgaaga gttcgacttt ggtgtgccga
    10861 cgcgaacgga atcggattgt tacgcgcggt atctggtgcg cgtggcggaa atgcgcgaaa
    10921 gcctgaagat catccgccag acgctggaaa agatttcgaa tgaagggccc atcatggccg
    10981 atgcgccagg catcgtcccg ccgcagcgcg aaaagatgaa gacggagatg gaggcgctca
    11041 tctaccactt caagattttc acggaaggat tttcgccggc gccgggcgag gtgtttgtca
    11101 cggtcgaatc gccacgcggg gagctgggct gttttgtcgc gagcgacggt tcgccgaagc
    11161 cgttgcgcgt gcacttccgt tccccttcgt atatcaatct gcaggcgctg ccgaagctga
    11221 tcgagggcca gttaatttcg gatgtgatcg cctgcatcgg cacgattgat atcgttctcg
    11281 gagaagtgga caggtaagca tggaactctc cccaacactt tcagcgaagt ttgaaaagtt
    11341 gcaatcgagc tacccggtga agcgaagcgc gctgatcccc atgctgatgt acgcgcagga
    11401 cgaatacggt tacatcagcg acgagatgat cgcggagctt ggccgccggc tggacctgaa
    11461 caacgtgcag gtcgaagaga cgctggcgta ttactcgatg ctgcaccgga agccgtgggg
    11521 aaagaaccac gtgcagatct gtacgaacgt ggcgtgcatg ctgaagggcg gatacagcat
    11581 gctcgaccgc gcgaagaagc gcctggagat tgggcacaag gaaacgacgc aggatggcgt
    11641 tttttcactg gaagaagtgg aatgcatcgg cgcttgcacg ggcgcgccgg ccatgcaggt
    11701 caactacgat ttcttcgaaa acctcacgcc gctcaagttc gatcgcatca ttgaggatct
    11761 ggatgcgggc agacgcccca cgccggagcc ggtgatatcc ggcgcgatcc acgagcgtca
    11821 tccagccgaa acgccgctga tcagccggcg gtggggcatg aaggactcgc acaagattga
    11881 ggtttacctg atgcagggcg gctatcaggc gctggagaaa gcgctgaaag agatgacgcc
    11941 cgaaagcgtg atcgacgaag tgaaaaagtc gaatctgcgc ggacgcgggg gcgcgggctt
    12001 ccccacgggc atgaaatggt cgttcgtgcc gaaagattcg ccgaagccga agtacgtgat
    12061 ttgcaacgcg gacgaatccg agccgggcac ctgcaaggac cggccgctaa tggagatgga
    12121 cccgcaccag ctcatcgaag gcatggtgat tgcggggcgt gccattggct cgcaccaggg
    12181 cttcatttat atccgcggag aataccgata cgtgctgaat atcatggatg cagccatcgc
    12241 cgaagcgtat gcgcacggct atctgggaaa aaacatcgcc ggctcggggt tcgatttcga
    12301 cctgctggct cacacgggcg cgggcgcgta cgaatgcggc gaagagtctg cgctgatgga
    12361 atcgctggaa ggcaagcgcg gctaccccag gatcaagccg ccgttccccg cggttgtcgg
    12421 gttgtacgga tgcccgacca tcatcaataa cgtggaaacg ctgagtgctg tgccgtcgat
    12481 catccgggag ggtgccaacg cgtatgcgaa tcgcggcaca ccgaaaaatg gcggcacgcg
    12541 gatgctgtgc attgcggggc acatcaacaa gccgggcatc tacgaagtgc ctctgggcat
    12601 gaacatgaaa aagtttatct acgaggtggc gggaggaatt ccgagaggaa agaaactgaa
    12661 ggccgtgatt cccggaggga gctcctgccc gctgctcacc gcagacgaga tcgacattgc
    12721 gatggactac gattcggtgg cgaaagcggg ttcgatgttg ggttccggcg gcatggtgat
    12781 catggacgaa gacacctgca tggtggacat ggcccgccgg atcatgcact tttacgcgca
    12841 cgaatcctgc ggctggtgta tcccctgccg agaagggacg atctggctgc ggaagatact
    12901 ggaacgtttt catgcgggcc tgggacgcgc ggaggacatt gacatgattg ccgagctggc
    12961 gaaaaacatg ctgggccgga cgttctgccc tctgggagac gcggcggcga tgccgacgat
    13021 cagcatcatg aagaaatggc gcggtgagtt tgaagaacat ttgaagggac gctgcgcgtc
    13081 caagtccgcc gaagcgctgg ttggatcacg ataggaatcc atggccgaac agaaactgat
    13141 caagctgaaa attaacgagc gcgaagtgca ggtgccgcag ggcacgctgg tgatcgaagc
    13201 gacgcggcgc atcgggacgg aagtgccgtc gttctgctac taccccgggc tatcgctgca
    13261 ggcggcatgc cgcatgtgtc tggtggaagt ggagaaagcg ccgaaactgc agaccgcctg
    13321 cacgctgatg gcgacggaag gcatgatcgt tcgaacggac acggagcagg tgcggcaggc
    13381 gcgcaaatac atgctggaat tcctgctgac gaatcacccg ctggattgcc cggtgtgcga
    13441 caagggcggc gagtgcgacc tgcaggattt ctccttctat cacggtgccg gcaagagccg
    13501 gttcatcgag gagaagctga agtaccccaa gccggtgccg ctgtcggaca agatcctgct
    13561 ggacatggag cgctgcgtgc tctgcgagcg ctgcgtgcgt tact
//
LOCUS       DASYVO010000411         4074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_80756, whole genome shotgun sequence.
ACCESSION   DASYVO010000411 DASYVO010000000
VERSION     DASYVO010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80756"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VGR03_13730"
     CDS             complement(<1..590)
                     /locus_tag="VGR03_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995385.1"
                     /translation="MKKSAVICSCALLAFVCVFARENRAAAQAKKKALASEAPLPQVD
                     KEKIRAHVKYLSSDELEGRGTGQRGGEIAADYIRKQFASYGLKPAGDNGTFFQEVPMV
                     GVKTLPETTFNFTEASGKTFEAKNLTEFVTNNESQTEIAEIDAPIVFVGYGIKAPEYD
                     WDDYKTVDLRGKVALVFVNEPSSDDLDFFKGKALTY"
     gene            complement(615..1079)
                     /gene="nrdR"
                     /locus_tag="VGR03_13735"
     CDS             complement(615..1079)
                     /gene="nrdR"
                     /locus_tag="VGR03_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEV1995386.1"
                     /translation="MKCPFCANIDDKVIDSREGRLGDTIRRRRECLKCGRRFTTYERI
                     DEIPYMVIKKDGRRERFERQKILQGLLKACEKRPVPTPKLEAIVDEIEGVVQESTERE
                     LTTTEIGEMIMHRLKKLDKVAYVRFASVYMDFKDVQEFMSELKNLLKDRVKK"
     gene            complement(1076..2170)
                     /locus_tag="VGR03_13740"
     CDS             complement(1076..2170)
                     /locus_tag="VGR03_13740"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEV1995387.1"
                     /translation="MPSSSRKLLVVLAGLLVLGVVVYQSSGFIHHANFSGEKLLHAVR
                     GANLSLLLLSVVAIYACYALRSLRWQLFQENLGVSRFSTIYKITLAGFAAVFLLGRAG
                     EPVRPLLLARKEKLPAADMFGIYALERLFDTASAAVIAAIALLLFQSHSHVGEMSSKL
                     EIAARTAGTLLFAGVGSAVAFLVYLRLHGTALLERWLRGWRASRGWRASVANIILGFA
                     RGVQAIRNWGELVLASAYSAAHWFLVLLVYVWVSHSFGGTLGTLSMGDAMLVMAFTLV
                     GSVVQLPAVGGGSQLASILVYTKVFGVETEAATAASIVLWLITFAAVSLAGVPLLIHE
                     GLSLGKLKEMAEHEKEAAIERAAQQGDSAQ"
     gene            complement(2228..2575)
                     /gene="mazF"
                     /locus_tag="VGR03_13745"
     CDS             complement(2228..2575)
                     /gene="mazF"
                     /locus_tag="VGR03_13745"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: protein motif:HMM:NF007386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HEV1995388.1"
                     /translation="MVAREYVPDAGDLIWLDFTPQTGREQAGRRPAVVLSPRNYNEKT
                     SLAVVCPVTSQVKGYPFEVSLPPGLRIRGAILSDHLKNLDWRQRQAQKAGRIPASVLA
                     QVRDRVAALLQIS"
     gene            complement(2569..2811)
                     /locus_tag="VGR03_13750"
     CDS             complement(2569..2811)
                     /locus_tag="VGR03_13750"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEV1995389.1"
                     /translation="MKTQLAKWGNSMAVRIPKAMAEAARLRPGDHLEMAVEDSGTLRI
                     RKKKGRQTLSQLVRAITPANLHGESDWGAPAGKELW"
     gene            complement(2863..>4074)
                     /locus_tag="VGR03_13755"
     CDS             complement(2863..>4074)
                     /locus_tag="VGR03_13755"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1995390.1"
                     /translation="VGGRMTTDRVNGFVVDRGVTLFGNAFGSMRALVKRLGLSPLVCK
                     GKFSVGIQDDAGCRGYRGGRFDDLLFDRAISWRARLASMRFGFDLLRHHRALVHGCST
                     LSGSLDGEDARTYFQRIGAEEVFERILRPGLNGPVGGAVETSSRVILMQVLWNLLVRG
                     QWNLTDGVDRIPEAAASQVKVLTETRALHVEQRDSGVQVEAIVKGKLQNIRARAVIFA
                     IPGQLVPTICPGLPEDTRDLLSRTQYSRIVNAAVALSRPPKVPYAGYAFTDDVVPGAE
                     IEMEHLRAPNRCPEGTGMAGVFLWDTPRLRRLDASEESLKQQASDVVEQNFPECRGKV
                     LFVHLVRWNIGIAQFPPGRLREMTALRKQLAAWNSPFDLCGDYLDGLSSEGALRTGEE
                     AAERTARKLGT"
BASE COUNT          871 a         1242 c         1109 g          852 t
ORIGIN      
        1 tagtacgtca gcgctttgcc cttgaagaaa tcgagatcgt ccgagctggg ttcgttcacg
       61 aagaccagcg ccaccttccc ccggaggtcg acagtcttgt aatcgtccca gtcgtactcc
      121 ggcgctttga ttccgtagcc cacgaaaaca atcggcgcgt caatttcagc aatttctgtc
      181 tggctttcgt tgttggtcac gaactcggta agatttttcg cctcgaaggt ttttccgctc
      241 gcttctgtga aattgaaagt agtttccgga agcgtcttca cgcccaccat cggcacttcc
      301 tggaaaaaag tgccgttgtc ccccgccggc ttcaacccgt acgatgcaaa ctgcttcctg
      361 atgtaatccg cggcgatctc gccgccgcgc tgccccgttc cccgcccttc caattcatcg
      421 ctcgaaagat atttcacatg cgcgcgaatt ttttccttgt ccacctgcgg caatggcgcc
      481 tcagacgcga gtgccttctt cttcgcctgc gccgccgcgc gattctcacg cgcgaaaacg
      541 cacacgaacg caagcaaggc gcacgaacaa atcacggcac ttttcttcat ggagactcct
      601 cgataaaaaa ggaattattt cttgacgcga tccttgagca agttcttcag ctcgctcatg
      661 aactcctgca cgtccttgaa gtccatatag accgaggcaa atcgcacgta ggcgacctta
      721 tccaacttct tgaggcggtg catgatcatt tcgccgattt cagtggtggt cagctcgcgt
      781 tcagtggact cctgcaccac gccttcgatt tcatccacga tggcttcgag tttcggcgtg
      841 ggcacaggac gcttttcgca cgctttcaaa agtccctgca aaatcttttg ccgctcgaag
      901 cgctcgcgcc gcccgtcctt cttgatgacc atgtacggaa tttcgtcgat gcgttcgtag
      961 gttgtgaacc ggcggccgca cttcaggcac tcgcgccgcc ggcgaatggt gtcgccgagt
     1021 cggccttccc gcgagtcaat caccttgtca tcaatgttcg cgcaaaaagg acacttcact
     1081 gggctgagtc tccttgctgt gcggcgcgtt cgattgccgc ttctttttcg tgctcggcca
     1141 tctccttcag tttcccgagc gaaagccctt catgaatcag cagcggcaca cctgccaggc
     1201 taaccgccgc aaacgtaatc agccacaaca caatcgaggc tgccgtagcg gcttctgttt
     1261 ctaccccaaa aactttggtg tatacaagta ttgatgccag ttgcgagccg ccgcccacgg
     1321 cgggcaactg cacgacggag cccactagcg taaatgccat caccagcatg gcgtcgccca
     1381 tgctgagtgt tcccagcgta ccgccgaaac tatgcgacac ccagacatac acaagcaata
     1441 ctaaaaacca gtgcgccgca gaatacgccg atgccagaac caactctccc caattccgaa
     1501 tcgcttgcac gccccgtgca aagcccagaa ttatattcgc gacgcttgct cgccagccgc
     1561 gtgacgcccg ccaaccacgg agccagcgtt ccaggagtgc cgtcccatgc aaccgcaggt
     1621 acactagaaa cgccaccgcg ctcccaactc cagcgaacaa gagagttccc gctgtcctcg
     1681 ccgcaatttc cagtttgctg gacatttccc caacgtgtga gtgcgattgg aacagcagca
     1741 aggcgatcgc cgcaattacc gctgcacttg ccgtatcgaa cagccgctct aaggcataga
     1801 tcccaaacat gtccgcagcc ggtagcttct cttttcgcgc tagcaatagg ggccgtactg
     1861 gttcgcccgc acgccccagg agaaataccg ctgcaaagcc ggcaagcgta attttgtaga
     1921 tggtcgaaaa tcgcgaaact cccaggttct cctgaaacaa ttgccaccgc agagatctca
     1981 gcgcgtaaca cgcatagata gcaacaacgg aaagcagcag caaagagaga tttgctccgc
     2041 gcactgcatg cagtaacttt tccccgctga aattggcatg gtgaatgaaa ccactcgact
     2101 ggtagacgac aactcccagc acgagcagcc cggccaggac aacaagaagc ttcctcgacg
     2161 atgatggcat gtgctggcca gcctagcggg agcgggcact tgcgtcaagg catgcgagca
     2221 acatttgtca ggaaatttgc agcaaggcgg cgacgcggtc gcgcacttga gccagaactg
     2281 acgccggaat tcttccggcc ttttgggcct gccgctggcg ccaatcgagg tttttcaaat
     2341 ggtcggaaag aatcgcacct ctgatgcgca atcccggcgg gaggcttact tcgaatggat
     2401 aacccttcac ttggctggtg accgggcaga caaccgcaag gctggttttc tcgttgtagt
     2461 ttcgcggaga caggacaacc gcgggcctgc ggcccgcctg ttccctgccc gtctgcggcg
     2521 tgaagtcgag ccagatcaaa tctccagcgt caggcacgta ttcgcgagct accatagttc
     2581 ttttccggct ggtgcgcccc aatcgctctc gccgtgcaga tttgcgggcg taatagcgcg
     2641 aacgagctgg ctcagtgtct gcctaccctt cttcttgcga attctcaaag tgccggaatc
     2701 ctcgacggcc atctccagat ggtcgccggg acggagcctg gcggcttccg ccatggcctt
     2761 gggaattcgc acggccatgc tattgcccca ttttgcgagt tgggtcttca cggcaagccg
     2821 ctccttgtag atacatagta tatacgaatg cgcactcgtg ggtcacgtcc caagttttct
     2881 tgcggtgcgc tcggcggctt cttctccggt gcggagggcg ccttcgctgg agaggccatc
     2941 gaggtagtcg ccacagaggt cgaagggaga gttccacgcg gcgagttgct tgcgcagggc
     3001 ggtcatttcg cgcaagcggc cgggaggaaa ttgcgcgatg ccgatgttcc agcggacgag
     3061 gtggacgaag aggactttgc cgcggcattc ggggaaattt tgttcaacga catcgctggc
     3121 ttgctgcttg agggattcct cgctcgcatc cagacggcgc agccgaggcg tgtcccaaag
     3181 aaatactccg gccatgcccg tgccctcggg gcaacggttt ggagcgcgga gatgctccat
     3241 ttcgatttcc gcgcccggga cgacgtcgtc cgtgaacgcg tatccggcat aaggcacttt
     3301 gggaggccgc gaaagcgcga cggccgcatt gacgattctg gagtactggg tgcgagagag
     3361 cagatcgcgc gtgtcttccg ggagtcccgg gcaaattgtg gggacaagtt gaccggggat
     3421 ggcgaagatg acggcgcgcg cgcgaatatt ttgaagcttc cctttcacta tggcttcgac
     3481 ttgaactcct gaatctctct gttcgacgtg caaggcgcgc gtttccgtca gaactttcac
     3541 ttgggaagcc gcggcttcag gaatgcggtc gacgccatcg gtgagattcc attggccacg
     3601 gacgagcaag ttccagagca cttgcatgag aatcacgcgc gaagaggttt cgacggctcc
     3661 tccaaccgga ccattcaggc cggggcggag aatacgctca aagacttctt ccgcgccaat
     3721 tcgctggaaa taagtccgcg cgtcttcgcc atcgagcgaa ccgctaagcg tgctgcagcc
     3781 atggacgaga gctcgatgat ggcgcagcaa atcgaacccg aaacgcatgc tggccaaacg
     3841 agcgcgccag gaaattgccc gatcaaagag cagatcgtca aagcgtccgc cgcgatagcc
     3901 gcggcaaccg gcgtcgtcct gaatgccaac gctgaatttg cccttgcaca ccagcggtga
     3961 aagacccaag cgcttcacca gagcgcgcat agagccaaaa gcattgccaa acagtgtgac
     4021 gccgcgatcg acgacgaacc cgttgacgcg atcggtggtc atgcggccgc caac
//
LOCUS       DASYVO010000412         1781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_147185, whole genome shotgun sequence.
ACCESSION   DASYVO010000412 DASYVO010000000
VERSION     DASYVO010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147185"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VGR03_13760"
     CDS             <1..455
                     /locus_tag="VGR03_13760"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEV1995391.1"
                     /translation="CTGAKIVTNDFNLNKVATVQGFSVLNVNQLANALKPAVLPGEPM
                     RVLILREGKEPNQGVAYLDDGTMVVVDGARRMINKSIDIVVTSVHQTTAGKMIFGRLE
                     ERPEQSAPPMRQAAAAGRGDGNGGVRSDASGRTEISPRLPGQPSLDRP"
     gene            679..1395
                     /gene="ispD"
                     /locus_tag="VGR03_13765"
     CDS             679..1395
                     /gene="ispD"
                     /locus_tag="VGR03_13765"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV1995392.1"
                     /translation="MSRIAAILPAAGLGTRMGIETPKQFLELDGTPIVIHSLRRIASC
                     PQVTDIIVATRADEVGRVEERIAREKFKQVVRVVKGGDSRQDSVAAALREVPNDTEIV
                     LVHDAVRPFVTAEQIARVIEEARRCEAAILGIPAMDTVKEVKRASLPEDVALITGTVP
                     RERIVLAQTPQAFATKLLKEAFARAQADGVHASDEAGLVERMGHDVHVVLGSESNMKI
                     TKPADMDLARFYLERERKKK"
     gene            1392..>1781
                     /gene="ispF"
                     /locus_tag="VGR03_13770"
     CDS             1392..>1781
                     /gene="ispF"
                     /locus_tag="VGR03_13770"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEV1995393.1"
                     /translation="MSTRCGIGYDLHRLAEGRKLIVGGIELPFDKGPVGHSDGDVLAH
                     ALCDALLGAAGLGDIGTHFPDTDPRWKGANSMLFLEHAKKLLDEQHFAIEHVDAVIIT
                     EKPKLGPHFPKMREVLAKALGVGTEKIH"
BASE COUNT          378 a          499 c          573 g          331 t
ORIGIN      
        1 ggtgcaccgg cgcaaaaatt gttacgaacg atttcaacct gaacaaggtg gccacggtgc
       61 aggggttctc cgtgctgaac gtcaatcagc tcgctaacgc actcaagcca gccgtgctgc
      121 cgggcgaacc tatgcgtgta ttgattctgc gcgaaggcaa ggagccgaac cagggtgtag
      181 cgtatttgga tgacggcacc atggtggtgg tggacggcgc gcggcggatg atcaacaaaa
      241 gtatcgacat cgtcgtcacc tctgttcatc aaacgacggc aggcaagatg atctttggcc
      301 gtcttgagga acgtccggag caatcggctc ctccgatgcg acaagctgcg gcggcaggcc
      361 gtggcgacgg caatgggggc gtgcgttccg atgcctcggg ccgaacggaa atttcaccgc
      421 gtcttcccgg gcagccatcg ctggatcgtc cataacgccc aatcttccct gaacccgacg
      481 ctggttaacg ggtagccctg gcagaaacgc ttctctccat tccatcgagt cgtccggtgc
      541 aggaagcgca gcggttccaa gttttgccgg aattgggacg gcgctgctgg cctatttcac
      601 cccacacgcc cgcttgaggt acactctgaa gtttgcgatt ccaagcttgg cctctttagg
      661 accggaaccg cacaaaacat gagccgaatc gccgccattc ttcccgctgc aggcctggga
      721 acgcgcatgg gcattgaaac gcccaagcag tttctggaac tggacggaac gccgattgtg
      781 atccacagcc tgaggcgcat cgcctcatgc ccgcaggtta cggatattat tgtggcgacg
      841 cgggcggacg aagtggggcg tgtcgaagag cggattgcac gggagaagtt caagcaggtt
      901 gtgcgcgtcg tgaagggtgg cgattcgcga caggattcgg tggcggcggc gctgcgcgaa
      961 gtaccgaacg acactgaaat tgtactggtg catgacgccg tgcggccgtt tgtgacggcg
     1021 gagcagattg cgcgggtgat cgaagaagcg cggcggtgcg aggccgcaat cttggggatc
     1081 ccggcgatgg acaccgtcaa agaagtgaag cgcgcgagcc tcccggagga cgtggcgctg
     1141 attaccggaa cggtcccgcg cgaacgcatc gtgctggcgc aaactccgca ggcgtttgcg
     1201 acgaaacttc tgaaagaagc ctttgcgcgt gcccaggccg atggcgtgca cgcctcggat
     1261 gaagcagggc ttgtcgagcg catggggcac gacgttcacg tcgtcctcgg ctccgagagc
     1321 aatatgaaaa tcaccaagcc cgcagacatg gacctcgccc gcttctacct cgagcgcgag
     1381 cgcaagaaga aatgagcacg cgctgcggga tcggctacga cttacatcgt ttggcggagg
     1441 ggcggaagct catcgtcggc gggattgagc tgccttttga caaaggaccg gtcgggcact
     1501 ccgacgggga cgtactggcg catgcgcttt gcgacgcgtt gcttggcgcg gcgggcctcg
     1561 gagatatcgg gactcacttt ccggacaccg atccgaggtg gaagggcgcg aacagcatgc
     1621 tcttccttga acatgctaaa aagctgctgg atgagcagca tttcgcgatc gaacacgtcg
     1681 atgctgtgat cattacggaa aaaccgaaac tcggccctca ctttcccaag atgcgcgagg
     1741 tactggcgaa agctctgggc gtcggcaccg aaaaaattca c
//
LOCUS       DASYVO010000413        10234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_265359, whole genome shotgun sequence.
ACCESSION   DASYVO010000413 DASYVO010000000
VERSION     DASYVO010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 10234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10234
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265359"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /gene="hpnE"
                     /locus_tag="VGR03_13775"
     CDS             complement(<1..1215)
                     /gene="hpnE"
                     /locus_tag="VGR03_13775"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HEV1995394.1"
                     /translation="MIVIGGGLAGLAAGVALAESGWRVRLFEQRPFLGGRATSYVLPD
                     GEHVDNCQHVTLGCCTNLEDFYGRIGAAGKIKFFDRLLFLDPQGRRGEIQAGFLPAPF
                     HLTGSFAAFAPLTLRDKLSIARAMLDILQTQGKPADIQVESGISMLEWLRRRGQTKRA
                     IERFWRVVLVSALDEELDRTDARFGVAVFWKAFLTNSTGYRMGVPAVPLANLYDGCKS
                     EIERRGGEVVLRATVRGLKMENGELAGVRFDEGREETADAYVFAVPHTALAELLPESV
                     KQSDPSLTNLDKIKVAPITGVHFWFDRPVMTEPFVTLLDTTTQWIFNKTALYADSNGK
                     ENNPVSGQYLQLVISASYDLLQKPRQEIIDLCLGEVWEALPAARNAELVKATVIKEAA
                     ATFSPEPGVDRWR"
     gene            complement(1160..3112)
                     /gene="hpnC"
                     /locus_tag="VGR03_13780"
     CDS             complement(1160..3112)
                     /gene="hpnC"
                     /locus_tag="VGR03_13780"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEV1995395.1"
                     /translation="MLLPGEIDITRHAPEPGCSPEVAQRYTRWLATHHYENFNVVSWL
                     LPKALHQHFYNVYAYCRWADDLSDELPDAARALELLAWWEHELDACYEGKPSPAVFVA
                     LRETIVAKDIPKQPFADLLKAFRQDQTVKRYPSWDAVLNYCVYSANPVGQLVLYLCGY
                     RDEQRQRLSDATCTALQLANFWQDVSRDLEIGRIYVPLDAAAAQGLSESDIVERRFDE
                     RYVRLMKDLIARTRALFAEGQPLAKMVDGRLSVDLEMFTRGGLAVLDAIEARGYDTLH
                     QRPAVSKVKQASLLGRALVAHLIGGNPRSSETAPSPAVVGAQHAAPLLSTVIADSYEE
                     CHRIARASHSNFYYAFFLLPRPKRDALAALYAFMRLVDDVSDEDQGLLAKQRGLAKWR
                     AALDQAINGQEQLFDGNAAMATPAPALSGASEVLLALVDTMQRYKIPARYLHDLISGA
                     EMDLTVQTYSTFDRLREYCYRVAGTVGLTCSHVFGFSDARALDLAEKLGLAFQLTNII
                     RDVHEDYRLGRVYLPEVDLQRYGISPGDFGRNEATLGVRELLRFESERAWQLYEEGSA
                     LLGLIDADSRGALWLLVHTYSALLSRIESVDFAVFGERVRLPKAEKMMFIAKARFGRH
                     TEENILEKRDRDRRRAGGTGSRRRVG"
     gene            complement(3173..4288)
                     /locus_tag="VGR03_13785"
     CDS             complement(3173..4288)
                     /locus_tag="VGR03_13785"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1995396.1"
                     /translation="MSTTTPKKMEVGEEVKAAVTHVTVPSKMTAAVLYGSEDLRIEKI
                     DVPALAADEVLVRVRLALTDGTDLKVWKRGYHAKMIQPPAVFGHELVGEIVAVGKRVD
                     ARWRIGTRVIAANSAPCLRCYHCRRGQENLCDDLLFNNGAYAEYMRIPGRIVVENMLE
                     VPHSVDDQSAALAEPLACVLRGIHEMEVRSGDTAAVIGCGPIGLNFVRMLSRRGVRVI
                     ALARRSAPLDVARHLGAVATINVTETDDVVAAVKALTEDQLGPDSVVEAAGNPATWQQ
                     ALAMVRRGGVVNFFSGLPSGTRVEIDPAAIHYSEIKLISPFHHTPRFFREALEAIRRG
                     DISARDFVTEEIRLADLPQAFERMKSRSGEIKLAVRP"
     gene            complement(4285..5391)
                     /locus_tag="VGR03_13790"
     CDS             complement(4285..5391)
                     /locus_tag="VGR03_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1995397.1"
                     /translation="MSRTGTTLEPQKTPGPIPKTMRAGVYREKGIVRVEEVPVPGVAD
                     GEVLIKVAACGICGTDIKKIYHAYVPPPQILGHEVAGSVVAIGRGVTKWKLGDRVMSF
                     HHIPCGACFYCERRLFSQCQQYKTTGLTGGFTPNGGGFAEYVKAMPWVAERGIVALPD
                     NVSFEEATFIEPINTILKAVQKARVAAGETVLIVGCGPIGLQLLMVAKLEGAKLYTSD
                     PMAVRRAKSLTLGALESFDPSDGKLVEHMKGRTEGRGADAVLVAVAHPSVVVDALAAA
                     RPGGRVLLFAANDPVTRIEFPAAAVGIDEKEILGSYSAAVDIQEAGAGLVLKKKLPVM
                     DIVTHRFPLARIQEGLDLAAKPTAESLKILITHA"
     gene            complement(5388..6194)
                     /locus_tag="VGR03_13795"
     CDS             complement(5388..6194)
                     /locus_tag="VGR03_13795"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995398.1"
                     /translation="MRILVTFAVETEFAPWRKRHEFKRHEIRIPHLSLDKPFYEMYEA
                     GVAGTDVDVLLTGIGWGDLFPNVAQKALRDLLKRKPGCCVSTGLAGGLNGELRPGEIV
                     AASQVVLRQGGGKILSNKRLLKVAESCGARIANTLITESHIVSEASSKSAMSKFGDFV
                     DMESYHILQIVTGTQIPAIAIRGISDAADRDLPVDFNKILGRDGRVRKRKLIREIARH
                     PLKIPGLIRFGLESEKAAHALADFLDHFIASLADYDGRVAASAYGEVVAR"
     gene            complement(6245..7264)
                     /gene="hpnA"
                     /locus_tag="VGR03_13800"
     CDS             complement(6245..7264)
                     /gene="hpnA"
                     /locus_tag="VGR03_13800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HEV1995399.1"
                     /translation="MTTLVTGASGFLGSHVARQLVARGDEVRVLLRASSTNRAIADLS
                     LEYVTGDLRDPASLDRAMKGVERVFHVAADYRLWAKRSQDIYDSNVGGTKNLLDAAKR
                     AGVEQLIYTSTVATIAVDRPQHPNEFTDAKLEEMVGHYKRSKWMAEREALDAAKSGLP
                     VIVAMPTTPVGPWDWKPTPTGKIILDFLNGKMPGYVETGLNFVGVEECAAGHLLVAEK
                     GKVGERYLLGGENLTLKAMLDILAKITGLRAPSLKIPHGLALGVAYASTAFSRLIGRE
                     PGIPVEGVKIARHMMFVDCARAQRELGFHAGPAAAALERAVRWYEANGYIAKSRAKRM
                     ARAAA"
     gene            complement(7261..8040)
                     /locus_tag="VGR03_13805"
     CDS             complement(7261..8040)
                     /locus_tag="VGR03_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995400.1"
                     /translation="MMRYPLRLTADLTMARLAQKSRFALDTSPVQFVDAAEILHHESN
                     HPVSHERIREIISSRSPVVWIGGSEPLHHPGVSHLVRAMTQTGHFVFLETDGTLLRRR
                     IHEFQPLSRFFVTVRFAPAAQRPASKGPRAAAWELSAEGLGAARLSGFLICVHARVRA
                     QTELGEMAELIQVARSLDVDGIVISPANGGANSANPDAAALQRKTAEARKLIGSKWWE
                     SFSRLVEPVVNGERNAVQSAEETGVRVEQKAHANEEGVRVA"
     gene            complement(8046..10055)
                     /gene="shc"
                     /locus_tag="VGR03_13810"
     CDS             complement(8046..10055)
                     /gene="shc"
                     /locus_tag="VGR03_13810"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEV1995401.1"
                     /translation="MASFVRMGEEIPVAPAPSQEQDWAKRLEKTIERGANHLLSLQAE
                     EGFWQGELEADTTLESDYIYYLHVLGKADPERIAKLANYVRRRQLPDGGWSIYPGGPS
                     ELNATCKAYFALKLAGDSTDARHMAQARETVHRLGGLEHSNSYVRFYLALIGAVGWEL
                     VPSIPPELMLLPNWFYFNIYDMSSWTRGIVIPMAILSSLRPDWRLPERARVDELFADP
                     TRKTAAFDWSEQLISWKNVFLALDRGLKLYEKLPWKPLRQRALREAKSWMLNHIERTE
                     GLAAIYPAMMNSIFALMALGHGPDDPLTWREIKEFSRFEIEEGDTIRMQPCVSPVWDT
                     CIAMVALEEAGLPADHPALVKAADWILSKQVLGPGDWQVKNKDAEPGGWAFEFRNDFY
                     PDVDDTAFVLMALQRVKYPDPKRMEGAVRRGIQWLLSMQNRDGGWGAFDRDNDQKFLC
                     NIPFADHNAMIDPSTADVTARVVECLGRFGWPAEHPAIQRAVKFLLKDQSSDGSWFGR
                     WGVNYVYGTSGVLRALETVSLTASDYCQRAVIWLRTVQKADGSFGESLASYEMPSTKG
                     QGPSTPSQTAWGLIGLLAGADLSDPSIVKAVSYLVHQQKDDGSWNEPDFTGTGFPGVF
                     YLKYHLYRNSFPVYALARYSNQLHRADEYVALKFQPSEFRLRSEF"
BASE COUNT         2067 a         3115 c         2992 g         2060 t
ORIGIN      
        1 ccgccagcga tcgactccgg gctcgggcga aaatgtcgct gcggcttcct tgatgacggt
       61 ggccttcacc aattcggcgt tgcgagccgc gggcagagct tcccaaactt ctcctaaaca
      121 gaggtcgatg atttcctggc gcggcttttg cagcaaatcg taggacgcgc tgatgactag
      181 ctgcaggtac tgtcccgaga cggggttatt ctcttttcca ttcgaatcgg cgtaaagagc
      241 ggtcttattg aagatccact gagtggtggt gtccaggagg gtcacgaacg gttcggtcat
      301 cacagggcga tcaaaccaga aatggactcc ggtgatcggg gcgactttga ttttgtccag
      361 attggtgagc gacggatcgc tctgcttcac gctttccggc agcaactcgg cgagcgcggt
      421 gtgcggcacg gcgaacacat aagcatcggc cgtttcttcg cgaccttcgt cgaagcgaac
      481 gccggcgagt tcgccatttt ccattttgag gccacgcacc gtagcgcgca gaaccacttc
      541 cccgccgcgg cgttcgattt cggatttgca tccgtcgtag agattggcaa gcgggacggc
      601 gggcaccccc atgcgatagc cggtgctatt cgtgaggaat gccttccaga aaacggcgac
      661 gccgaagcgc gcgtcggtgc gatcgagttc ctcgtcgagg gcgctgacga ggacaacgcg
      721 ccagaaccgt tcgatggcgc gtttggtctg cccgcggcgg cggagccact ccagcatgga
      781 gatgccgctt tccacctgga tgtccgcagg ctttccctgg gtctgcaaga tatcgagcat
      841 ggcgcgcgcg atggaaagct tgtcccgcaa ggtcagcggc gcaaaggccg cgaacgatcc
      901 ggtcagatga aatggcgccg gtaagaaccc ggcttgtatt tctcccctgc gtccttgtgg
      961 atcgagaaag aggagccggt cgaagaattt gatttttccg gcggccccga tgcgcccgta
     1021 gaaatcttcg aggttggtgc agcagccgag agtcacgtgt tggcaattgt ccacgtgctc
     1081 gccgtccggc aacacgtagg acgttgcccg gccgccgagg aagggacgct gttcaaacag
     1141 acgtacgcgc cagccggatt cagccaacgc gacgccggct gccagtcccg ccagcccgcc
     1201 gccgatcacg atcacgcttt tcaaggatat tctcttccgt gtggcggccg aagcgcgcct
     1261 tggcgatgaa catcatcttt tccgctttgg ggagccgtac ccgctcgccg aatacggcaa
     1321 agtccaccga ctcgatacgg ctcaacagcg cgctgtaggt gtgaaccagc agccacaacg
     1381 cgccgcggct gtcggcatcg atgagcccga gcaatgcaga accttcttca tagagctgcc
     1441 aggcgcgttc ggattcgaag cggagcagct cgcgaacgcc gagagttgct tcgttgcggc
     1501 caaaatctcc cggagaaatt ccgtaacgct gcaagtctac ttcggggaga tagacgcggc
     1561 cgagtctgta gtcctcgtgc acgtcgcgaa taatgttcgt caattgaaat gcgaggccca
     1621 gcttttccgc caagtccagg gcgcgcgcgt cgctgaagcc gaacacgtgc gagcaggtca
     1681 gtcccaccgt gccggccacg cgatagcaat actcgcgcag gcggtcgaac gtcgaatacg
     1741 tctggaccgt tagatccatc tcggcgccgc tgatgagatc gtggagatag cgagccggga
     1801 ttttgtaacg ctgcatggtg tctacgaggg cgagtaaaac ttcggacgcg ccgctcaggg
     1861 ccggcgcagg cgtggccatc gcagcgttgc catcgaagag ctgctcctgc ccgttgatgg
     1921 cttgatcgag agcggcgcgc catttcgcca ggccgcgctg ctttgcgagg agcccttgat
     1981 cttcatccga gacgtcgtcc acaaggcgca tgaacgcata gagagcggcc agcgcgtcac
     2041 gcttcggcct cggtagaaga aaaaaggcgt agtagaaatt gctgtgggaa gcgcgggcga
     2101 tgcgatggca ttcttcgtag gaatccgcga tcaccgtgct caataggggc gcagcatgct
     2161 gcgcccctac aactgctggc gacggcgcgg tctcacttga acgcggattt ccaccaatca
     2221 agtgcgccac gagggcgcgc cccaagagtg aggcttgctt cactttgctg acggcggggc
     2281 gttggtggag agtgtcgtat cccctcgcct cgatggcatc gagcacggcc aaaccgccgc
     2341 gggtgaacat ttcaaggtcc acgctgaggc gtccatccac catttttgcc agcggttggc
     2401 cttcggcgaa tagcgcgcga gtgcgcgcga tcagatcctt catcaagcga acgtagcgct
     2461 catcgaagcg ccgctcaacg atgtcgctct ctgaaagacc ctgcgccgcg gccgcgtcga
     2521 gcgggacgta aatgcgtccg atttccaagt ctcgggagac atcctgccag aagttcgcga
     2581 gttgcagcgc cgtgcaagtg gcatctgata atcgctggcg ctgctcgtct cgatagccgc
     2641 agagatacag caccaattgt ccgactgggt ttgcggagta cacgcagtag ttcagaactg
     2701 cgtcccagct gggataacgc ttcacggtct ggtcttgacg gaaagctttc agcagatcgg
     2761 cgaacggctg cttgggaatg tctttggcaa cgatggtctc gcgcagcgcc acgaacacgg
     2821 cgggcgacgg ttttccttcg taacaggcat ccagttcatg ttcccaccag gcgagcaatt
     2881 cgagcgcccg cgccgcgtcc ggcagttcat cgctgagatc gtcggcccag cggcaatagg
     2941 catagacgtt gtagaaatgc tggtggagcg ctttggggag gagccaggag acaacgttga
     3001 agttttcgta gtgatgcgtg gccagccagc gcgtgtaccg ctgcgccact tcgggcgagc
     3061 agccaggctc aggggcgtgc cgcgtgatgt cgatttcgcc ggggagcaac atcagaagag
     3121 agattcctcg cttcgctcgg aatgacgggt gggtttggtt tgcacctgaa aactacggcc
     3181 gcacggccag cttgatttcg ccgctgcgcg acttcatgcg ctcaaacgcc tgcggcaggt
     3241 ccgccagccg gatttcctca gtgacgaaat cgcgagccga aatgtccccg cgccgaatgg
     3301 cttccagcgc ctcgcgaaag aatcgcggcg tatgatgaaa gggtgagatc agcttgattt
     3361 ccgagtagtg gatcgccgcc gggtcgatct ccacgcgggt gcccgacggc aggccgctaa
     3421 aaaaattaac aacgccgccg cgccgcacca ttgccaaggc ctgctgccaa gtggcaggat
     3481 tgcccgccgc ttcgaccacg gaatccggtc caagctggtc ttcggtgagc gcttttacgg
     3541 cggccacgac atcgtccgtc tcggtgacgt tgatggtcgc cactgcgccc aggtgcctgg
     3601 caacatccag cggcgccgaa cgccgcgcca gggcgatgac gcgaactccg cgccgcgaaa
     3661 gcatacggac aaaattcaat ccgatgggtc cgcagccgat cacggctgcg gtatccccgg
     3721 agcgcacttc catctcgtgg attccgcgaa gcacgcaggc caggggttcg gccagcgcgg
     3781 cgctttggtc atccaccgaa tgcggcactt ccagcatatt ttcgacgacg atgcggccgg
     3841 ggatgcgcat gtactcggcg taggccccgt tgttgaacaa caggtcgtcg caaagatttt
     3901 cctggccgcg gcggcagtga tagcaacgca agcaaggcgc ggaattcgcg gcgatgacgc
     3961 gcgtcccgat ccgccagcgt gcatccacgc gcttgcccac cgccacgatt tcgccgacca
     4021 gttcgtggcc aaagacagcg ggcggttgaa tcatttttgc gtgataaccg cgcttccaaa
     4081 ctttgaggtc cgtgccgtcg gtaagcgcta gccggacgcg caccaggact tcgtctgccg
     4141 ccagagcggg cacgtcaatc ttttcaatgc gaagatcttc gctgccatag agcaccgccg
     4201 cagtcatctt cgacggtacc gtgacgtggg ttacggcggc tttcacttct tcgcctactt
     4261 ccatcttctt tggcgttgtc gtgctcatgc gtgtgtgatc aagatcttca gtgattccgc
     4321 cgtcggcttg gcggccagat ccaacccctc ttggattcta gccaaaggaa aacggtgcgt
     4381 cacaatatcc attaccggca gtttcttctt cagcaccagg ccggcccctg cttcttggat
     4441 gtctacggcc gcactatagc tgcccaggat ttctttttcg tcgattccca ccgccgcggc
     4501 gggaaactca atcctggtta ccgggtcgtt cgcggcgaac aacagcacgc gtcctccggg
     4561 ccttgccgcc gccagcgcat cgaccacgac agacgggtgc gccacagcta ccaataccgc
     4621 atcggcgcct cgaccctcag tccggccctt catgtgttcc acaagcttac catcactggg
     4681 gtcaaacgac tccaatgctc ctaaagttag actctttgcg cgccgcacgg ccatggggtc
     4741 actggtatag agctttgcgc cttcgagctt ggccaccatc agcaattgca ggccgatggg
     4801 gccgcaaccc acgatcagca ccgtttcgcc tgccgctacg cgggccttct gcacggcctt
     4861 caaaatcgtg ttgatcggct cgataaaggt tgcttcctcg aagctgacgt tatcgggcag
     4921 ggcaacgatt ccgcgttcgg ccacccaggg catggctttc acgtattcgg caaatccccc
     4981 gccgttgggg gtgaatccgc cggtcagacc cgtcgtcttg tactgctggc actgcgagaa
     5041 gagtcggcgt tcgcaataga aacacgcgcc gcagggaatg tgatgaaaac tcataacgcg
     5101 gtcgcccaac ttccacttcg tcacgccgcg accaatggct acgacactgc cagctacttc
     5161 atggcccaga atctgcggcg ggggaacata ggcgtgataa atttttttga tgtcggtgcc
     5221 gcaaatgccg caggcagcaa ctttgatgag gacttcgccg tcggcgactc cgggtacggg
     5281 gacttcctcg acgcggacga ttcccttttc gcggtagaca cccgcgcgca tggttttggg
     5341 gatggggccc ggcgtcttct gaggttccag agttgttcca gtccggctca tcgcgccacc
     5401 acctcaccat atgcagatgc tgcgactcgt ccgtcgtagt ccgcgagtga agcaatgaaa
     5461 tgatctagaa aatccgctag cgcgtgggca gccttctcac tttccaaacc aaaacgaata
     5521 aggccaggta tcttgagcgg gtggcgagcg atttctctaa ttaattttcg ttttcttact
     5581 ctgccatcgc ggccgagaat cttgttgaaa tccactggca ggtcgcggtc agccgcatcg
     5641 ctgatgcccc gaattgcaat cgccgggatt tgagtgccgg tcacaatttg caatatgtga
     5701 tagctctcca tgtccacaaa gtcaccaaac ttggacatcg cggatttgga cgaagcttca
     5761 ctgacgatgt ggctttcagt gatcagtgta ttggctattc tagcgccgca gctctccgcg
     5821 acttttagga gccttttgtt gctcagaatc ttaccgccgc cctgacggag aactacttga
     5881 gaagcggcaa cgatctctcc cggacgaagt tcgccgttca acccgcccgc cagtcctgtt
     5941 gaaacacagc aaccaggttt tctcttcagc aaatcacgca atgctttttg cgcgacattg
     6001 gggaataaat ccccccagcc gattcccgtc aatagaacgt caacatcggt tccagccaca
     6061 cctgcctcat acatttcata gaagggtttg tccaaggaaa ggtgcggaat cctgatttca
     6121 tggcgtttga actcatgccg cttgcgccag ggggcgaatt cagtttcgac ggcgaatgtg
     6181 acgaggatgc gcattgtctc aaagatcgaa agacaccggt ctgaagaccg gccactacaa
     6241 aatcctaggc ggcggcgcgg gccatgcgct tggcgcggct cttggcgatg tagccgttcg
     6301 cttcgtacca gcgcaccgcg cgctccagcg ccgcggctgc cggcccggcg tgaaatccca
     6361 attcacgctg ggcgcgcgcg caatccacaa acatcatgtg ccgggcgatc ttgacgcctt
     6421 cgacgggtat gccaggctcg cgcccgatga gccttgaaaa cgccgtgctg gcataggcta
     6481 cacccagggc gagtccatga ggaattttca gactgggcgc gcgcagaccg gttatcttcg
     6541 cgagtatgtc cagcatcgct ttgagcgtca gattctcccc gcccagcaga tagcgctcgc
     6601 cgactttccc tttttccgcg accagcagat gcccggccgc gcactcttcc acaccgacaa
     6661 aattcaagcc ggtctccacg taaccgggca tcttgccatt caagaaatca agaatgatct
     6721 ttcccgttgg cgtcggcttc cagtcccacg gccccacggg cgttgtcggc atggccacaa
     6781 tgaccggcaa tccgctttta gcggcgtcga gcgcttccct ctccgccatc catttcgagc
     6841 gcttgtagtg gcccaccatt tcttccagct tggcgtcggt gaattcgttc gggtgctgcg
     6901 gccgatcgac ggcaatggtc gctaccgtgc tggtgtagat caactgctcg acgccggcgc
     6961 gcttcgcggc gtccaaaaga tttttcgttc cgccgacgtt cgaatcgtaa atatcctgag
     7021 agcgcttggc ccacaagcgg taatccgcag cgacgtgaaa gacgcgctcg acgcccttca
     7081 tcgcacgatc gagcgaggca gggtcgcgca aatccccggt tacatactcg agggacaaat
     7141 ccgcaatcgc gcgattggtg ctcgacgcac gaaggagcac gcgcacttcg tcaccgcggg
     7201 ccaccaactg ccgtgccacg tggctcccca ggaagcccga cgcacctgtg accagcgtgg
     7261 tcatgctacc ctcacgcctt cctcgttcgc gtgtgctttc tgttctacgc ggacaccagt
     7321 ctcttcggca ctctgcacgg cattccgttc gccgttcact acaggttcaa caagacgaga
     7381 aaaggactcc caccacttgc taccaatcag ctttcgcgct tccgccgttt tccgctgcag
     7441 ggccgcggcg tccggattcg cggagttcgc accgccgttt gccggcgaaa tcacaattcc
     7501 atccacatcg agtgagcgcg caacctgaat taactcggcc atctctccca gttcggtctg
     7561 cgcgcgaacg cgcgcatgca cgcagatcaa aaagcccgag agccgcgccg cgccgaggcc
     7621 ctccgcggac agttcccatg cggccgcccg tgggcctttc gaagcgggac gctgcgcagc
     7681 aggtgcaaag cgaaccgtta caaagaaccg cgacaacggc tgaaactcgt gaatgcggcg
     7741 acggagcaag gttccatcgg tttcgagaaa aacaaagtga ccagtctgcg tcatcgctcg
     7801 aaccagatgc gaaacgccag gatgatgcag gggctcgctt ccgccgatcc aaacaaccgg
     7861 agagcggctg gaaataattt cgcggatcct ctcgtgcgaa accggatgat tggactcgtg
     7921 atgcagaatt tctgccgcat ccacaaattg aacggggctg gtgtccaggg cgaaacgcga
     7981 cttctgcgcg agccgggcca tggtgaggtc cgccgtaagc cgcaggggat accgcatcat
     8041 gcgctctaaa actcgctgcg cagccggaat tcgctgggct ggaacttcag agcgacgtac
     8101 tcatccgctc tgtgcaactg gttggagtag cgtgccagcg cataaaccgg aaaggaattc
     8161 cggtagaggt gatacttcag gtaaaacacg cccgggaaac ccgtgccggt gaaatccggc
     8221 tcgttccatg agccatcgtc cttctgctgg tgcacgaggt aggaaaccgc tttgacgatc
     8281 gaggggtcgc tgagatccgc gccggcgagc aaaccgatta atccccaagc cgtctgcgaa
     8341 ggcgtgctcg ggccttgacc tttggtggag ggcatttcgt agctggccag cgactcgccg
     8401 aagctgccgt cggccttctg caccgtgcga agccagatca ccgcgcgctg gcagtaatcg
     8461 ctcgcggtca gcgaaaccgt ttccaaagcg cgcaagacgc cgctcgttcc gtaaacgtag
     8521 ttgacgcccc agcggccgaa ccaggaaccg tcgctggatt gatccttcaa caaaaacttc
     8581 acggcgcgct gaatggcggg atgttccgcg ggccagccaa acctgccgag gcattcgacg
     8641 acgcgcgcgg tgacatcggc ggtcgaagga tcgatcatcg cgttgtgatc cgcaaaagga
     8701 atgttgcaga gaaacttttg gtcattgtcg cggtcaaacg cgccccagcc gccgtcgcga
     8761 ttctgcatgc tgagcaacca ctgaatgccg cggcgtacgg cgccttccat gcgcttgggg
     8821 tcgggatact tcacacgctg aagcgccatc aacacaaacg cggtgtcgtc gacgtcggga
     8881 taaaaatcat tgcgaaattc aaaagcccag ccgccgggtt cggcgtcttt gttcttcact
     8941 tgccaatcgc ccggcccgag cacctgcttc gagagaatcc agtccgcggc cttcaccagc
     9001 gcgggatgat ccgcgggcaa gccggcttct tccagagcga ccatggcaat gcaggtatcc
     9061 caaaccggcg aaacgcacgg ctgcatgcga atggtgtcgc cctcttcgat ttcaaatcgc
     9121 gaaaactcct tgatttcccg ccaggtcagc ggatcgtccg ggccgtgtcc cagggccatc
     9181 agcgcgaaaa tcgaattcat catcgccggg tagatcgcgg cgagtccttc ggtccgctcg
     9241 atatgattca acatccagga tttcgcttcg cggagtgcac gctggcgcag cggcttccag
     9301 ggcaatttct cgtagagttt caatccgcga tccaacgcca ggaaaacgtt tttccaggag
     9361 ataagctgct cgctccaatc aaaagccgcg gtcttgcgcg tggggtccgc aaacaattca
     9421 tcgacgcgcg cgcgctccgg caagcgccaa tcgggccgca gggaggacag gatggccatg
     9481 ggaattacga tgccgcgcgt ccaggaagac atgtcgtaaa tgttgaaata gaaccagttc
     9541 ggcaggagca tcagctcggg cgggatcgat ggaacgagtt cccatccgac ggcgccgatt
     9601 agtgccaggt agaaccgcac gtaggaattg ctgtgctcga ggccaccgag acggtggacg
     9661 gtctcgcgcg cttgcgccat atggcgcgca tccgtggagt cgcccgccag cttcaaggcg
     9721 aaatacgctt tgcaggtagc gttcagttcc gaaggcccgc cgggatagat ggaccagccg
     9781 ccatcgggga gttgccggcg gcgcacgtaa tttgccagct tggcaatgcg ctccggatca
     9841 gcctttccca gaacgtgcaa gtaatagatg taatcggatt cgagggtggt gtccgcttcg
     9901 agctcgccct gccagaatcc ttcctctgct tgaagggaaa ggaggtgatt cgcgccgcgc
     9961 tcgatggttt tttccagacg cttcgcccag tcttgttcct gcgaaggcgc gggcgcgacg
    10021 ggaatctctt ctcccattcg gacaaatgag gccatcagtg ctccccccgg agcggaactt
    10081 ccagctggca tgtccagcgg tctgtacagc caacattaaa atcagtttaa ctgccgcgtt
    10141 gcggatcact tacccacaaa agcggcgcgg cagttagggt tcgagctaca cgaatacttt
    10201 taaattatcg ccgccttcgc tgtgacggtc aacg
//
LOCUS       DASYVO010000414        11957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_65858, whole genome shotgun sequence.
ACCESSION   DASYVO010000414 DASYVO010000000
VERSION     DASYVO010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 11957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11957
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65858"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..2883
                     /locus_tag="VGR03_13815"
     CDS             205..2883
                     /locus_tag="VGR03_13815"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV1995402.1"
                     /translation="MALAAGTKLGPYEIQSPLGAGGMGEVYRARDTRLDRTVAVKILP
                     TRLSDSPEAKQRFEREARAISSLSHPNICHLYDVGSQDRIDYLVMEYLEGETLARRVE
                     RGPLPTEQALRVATEISDALEKAHRQGVIHRDLKPGNIMLTKSGAKLMDFGLAKGTMP
                     PPVMDPTALTAMTNGQPLTVEGTIVGTLQYMSPEQLQGREADARSDIFAFGAVLYEMI
                     TGKRAFPGKSHSSVMSAILEKDPEAITAGQPLAPPALEHVIRTCLAKDPDERFQTAHD
                     VKLQLKWIAAGAPQLAMPAMRSNRERGIWIFAVVTLLVAMAAVYFHTSSERTQTTWSS
                     ILAPENTRFAYFAGPVTVSHDGRKLAFVATTSAGQDVVWVRPLGALNAQALPGTEAAS
                     FPFWSGDDRSIGFFARGKLKTIEAAGGPVVTICDAPGARGGTWNQSGVILFAGTWGGI
                     YRVPSSGGTPSEITKLDRSHGELTHRWPFFLPDGRHFFYLAANFMSGNKEANSVSLGS
                     LDSTETKLLFHARSNAAYTSGYVLYVRDRTLMAQGFDAKQLELRGQPLPIAGQVQFDE
                     LTGRGVFSGAANGTLAYQGENTGANSRMVMFDRAGKEVKTIGTPADFVHHKISPDGQR
                     LAVTVLDPSVVNYKLWLYDLFREKETRLTFGPDRITNPVWAPEGNTLVFGSNKRGPSD
                     IFEKRADSAGSEELVLQSDANKYPTDWSADGRFIAYSVSVGKSKIEVWILPRFGERKP
                     YIFLQGDFNMGEGRFSPDGRWLAYTSDESGRAEVYVTPFPSGASKWQVSVAGGTNPRW
                     RRDGKELFYLAADSELTAAQVDTAGSVFQVGAVRPLFHMLLRTGPSRLELSPTSGQIG
                     YDAAPDGKWFVVNSPLAGSPPPITLVTNWAAELKKQ"
     gene            2990..3481
                     /locus_tag="VGR03_13820"
     CDS             2990..3481
                     /locus_tag="VGR03_13820"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1995403.1"
                     /translation="MRRTSVFVTIAFLAGLGVGFFAGSAYPGMLQRRTHGADLAAIEK
                     LERADVEATLTQDPSLLINLWSDDGVKLDVPGPPVVGKKAIQELYQKARTVYPEFKVL
                     KYAPDFKDVQIADGWALEWGYFEAMYKMSAKDNPVNVKGKGARVLKRQSDGSWKLAVV
                     GLK"
     gene            complement(3534..4397)
                     /locus_tag="VGR03_13825"
     CDS             complement(3534..4397)
                     /locus_tag="VGR03_13825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV1995404.1"
                     /translation="MSKRIGLPVTLKMRHDAHYVESLTSYSGAAVGRMIPIEKIQPNP
                     EQPRKALGELRELTESIREKGVLEPLLVRYVPREDGYHIISGERRYHAARAAGLREVP
                     CIEKIADDAETLEIALIENLQRKDLTAFEEAEGLARLAQQFDYTHEDIAKKISRARSS
                     VTETLSLCAITGAARQKCLEHGILSKSLLLQVARQPSERKMLEMVERIARGGVNREQA
                     RAERRDEKSAGPRPQPFIFQFEPDTRQFRLRLQFQKSQVSRIELITALRAVLSELEAT
                     VQEEDSATTAA"
     gene            complement(4351..5151)
                     /locus_tag="VGR03_13830"
     CDS             complement(4351..5151)
                     /locus_tag="VGR03_13830"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEV1995405.1"
                     /translation="MTWVLAIANQKGGVGKTTTAINLAAALAERKKRTLLIDLDPQAN
                     STLAFFNQDEIHTTMHEVLGEQRTSLASVIRPTKDANLKMAPGRLSLARLEVQLAGQF
                     DAPFKLKDALSPVLKDYDYVVLDTPPALGILTVNALVAASHLLVPIQAAYFAIEGTDD
                     LLETYSRIRARPNPDLKILGVLITLYDKRTNISRDTHEQIRATFGPTLFKTKITKNVR
                     LEESPAYKETILKFAPKSPGAVEYRKLALEVIHRVETDRSARHAEDAA"
     gene            5437..6381
                     /locus_tag="VGR03_13835"
     CDS             5437..6381
                     /locus_tag="VGR03_13835"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEV1995406.1"
                     /translation="MNATTDCRIVTIACRFPLAAVLFLAVFAPAAAQQPPAPSASAAV
                     SPATAPTAAYSESSSGLEKLMKDILRATKDGDTVKSRALLSSLALPDPEAWFRGVFGS
                     DAPPMLREHPQSPPVMERALIQLFSKLLKEKFTDVRVQKHEKTCDDNSGEQIYPVLVM
                     RQSPVPLYEVRFQSGTNFYRLWAVAYADGGFRFAGHLHVPESFFPPEIAGGQSVKSII
                     KRISVAGNVAKAKLVRQVTPRYPEAAKQEHLQGTVRLHAIIQKDGSIRQLRVLTGYCS
                     LSEASIEAVRQWRYRPTLLNGEPVEVDTIIEVIFQLRS"
     gene            complement(6424..6777)
                     /locus_tag="VGR03_13840"
     CDS             complement(6424..6777)
                     /locus_tag="VGR03_13840"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995407.1"
                     /translation="MTPAPLKPAISMEILNQVDIRVGTIEAVDDVPEADKLLQMRVNF
                     GDHTRTIVAGMKQERANPQKIVGRQALFVVNLEPRKMRGVVSEGMLFDIGDADGVKPV
                     LAVPEAPVPDGTRAE"
     gene            complement(7068..7640)
                     /locus_tag="VGR03_13845"
     CDS             complement(7068..7640)
                     /locus_tag="VGR03_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995408.1"
                     /translation="MNKRKFGTRSPAGIHDDVNDSSEGGGKSMHKKYSSKTASSIFLF
                     CVTFAVMAIPVLGQNYPPNDPRGSYYRQAFDQGFRAGQADFQARRASNYNAALVTTAG
                     VAAISRDYRNAFKLGYQDGYAGHNRQSDWYYQHHQHNADCKEEDNRGPENWGCQERHD
                     NGKHKGWYKKHRPKDDDHEDDDRKKDKDSD"
     gene            complement(7722..8207)
                     /locus_tag="VGR03_13850"
     CDS             complement(7722..8207)
                     /locus_tag="VGR03_13850"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEV1995409.1"
                     /translation="MEVEMKIRGLMMDPVTNMPIVVLKDAQGTAILPIWVGIYEANAI
                     ALEIEKVQTPRPMTHDLLKNLLTGLEVRVHKVVVSDLKDDTFFALIWMEREGKLMSMD
                     SRPSDALALALRLDCPIFVDDEVLKNSKVASAVTEKTTNEELRKWLEGLNDEDLGRYK
                     M"
     gene            complement(8244..9779)
                     /gene="miaB"
                     /locus_tag="VGR03_13855"
     CDS             complement(8244..9779)
                     /gene="miaB"
                     /locus_tag="VGR03_13855"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEV1995410.1"
                     /translation="MSQPFDIRADALIGRGVSLAAATNAALMGEATRRKQEEDAALKG
                     SATQGKAFYIETFGCQMNEHDSEKVSGVLMSRGYRMVNDPAAADLVFYNTCSIREKAA
                     QKVFSRLGLFNPNNAPTGDPDVAPADATPGRHIPIKYLGGAPGTRKIVGVLGCVAQQE
                     GEEIFERAPWVSLVCGSASYRKLPELLAQLESGNRRVMGLDTDTDETFETEITRRDNP
                     FRAYLTIIEGCDKACAYCVVPFTRGPERSRPSDRVLREVRDLADIGYSEIQLLGQTVN
                     SYRDPSPRKVTFAELLTAVAQVRGIRRVRFTTSHPRDFHREIVDAIDTHPQLCDHIHL
                     PVQSGSTRLLEKMLRTYTREEYLGKIAMIRAARRAISVTTDIIVGFPGETEEDFQQTL
                     TLLDAAQYDGVFAFKFSPRPNTPALAMQDGIPEAEKDRRLAVLLDRQRQIQMLRNEQL
                     VGSSFEVLTDNRGHKEAQWSGRTSSNRVVNFTSPRTALMGEYLPVRITRAGPNSLVGE
                     QVV"
     gene            complement(9898..10374)
                     /locus_tag="VGR03_13860"
     CDS             complement(9898..10374)
                     /locus_tag="VGR03_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995411.1"
                     /translation="MQSMSQLVLNVEHTALEHRNSEGQLLWQCPLDSIVLMAEYTTNE
                     GPFLDDYFLVFVSIEKGKQYFATASFYSDGREMIVEHLSQKWDVRVKLGLSHSTEWKS
                     RVLWPPEIAGNDYFSFREIQSDSRLEKLRGFAFGPAREYYPSQEVCEFLDSRCPKQ"
     gene            10680..>11957
                     /locus_tag="VGR03_13865"
     CDS             10680..>11957
                     /locus_tag="VGR03_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995412.1"
                     /translation="MSKRARVFVTKITVTKFAVAKYIVMIGVAALLAVALLATPGLMQ
                     AQQAQGNSQAGSQVGPDVYGQIKYRYIGPEGNRATSVAGVPGNPNIYYVGAASGGIFK
                     SSDGGIHWEPIFDGQPVASIGSLAVAATDANIVWAGTGESFIRSHISVGAGIYKSTDA
                     GKNWTLMGLEKAGRIGRVEIDPRNPDVVLACALGHAYGPQPERGVFRTTDGGKTWERV
                     LFVDENTGCSDMGMDPNNSRILFAGMWQIEIHTWGRTSGGPGSGLFKSTDGGATWKRL
                     EGHGLPHSPVGRIAVRVAKKDSSRVYAEIETGDGVPQPNSPGQLGQLWRSDDGGENWQ
                     MVNSDRQLRGRTHYYTRLEIAPDNENEAFFFSGSFSRTLDGGHSLTKMPASPGGDFHE
                     MWIDPANGDRMAVVWDGGVSISVNRGHSWNSVQF"
BASE COUNT         2301 a         3535 c         3694 g         2427 t
ORIGIN      
        1 caatttcgta gggacccagc ttggtgccag aggtcagtgc catgggcgcc ccggattata
       61 gcgtcagaat tccgcacaca ggcatgttgc tttgcgcagg ggcacgaccc gccagaacca
      121 cggcgggtgc ccctacgctc gcgatccaat acgcgcatga attccgatat cccatcgttg
      181 accacggtat aatccgtctc gccgatggca ctcgccgcgg gcacaaaact cggtccttat
      241 gagatccaat ccccacttgg tgcgggtggc atgggagagg tgtatcgcgc ccgggacacg
      301 cgcctggatc gcaccgtggc ggtcaagatt ctacccacgc ggctttctga cagcccggaa
      361 gccaagcaac gcttcgaacg cgaggcgcgg gccatttcgt ccctgagtca tcccaacatc
      421 tgtcatctct acgacgtcgg tagtcaggac cggattgatt acctggtcat ggagtacctg
      481 gaaggggaga cgctggccag gcgggtggag cgcgggcctc tgcccaccga gcaagcgctg
      541 cgcgtggcca cagaaatttc cgacgcgctg gagaaggccc accggcaagg cgtcatccat
      601 cgcgatttga aacccgggaa catcatgctg accaagagcg gagcgaagct gatggatttc
      661 ggtctggcca agggaaccat gccgccgccg gtgatggacc ccacggcctt gaccgcgatg
      721 accaacgggc aaccgcttac cgtggagggc acgatcgtcg gcactttgca gtacatgtcg
      781 ccggagcaac tgcaggggcg cgaagccgat gcgcgcagcg acatcttcgc ctttggcgcg
      841 gtgctgtacg aaatgatcac cggaaagagg gcgtttccgg gcaaaagcca cagcagcgtg
      901 atgtccgcca ttctggaaaa agacccggaa gcgatcaccg ctggccagcc gctggcgccg
      961 ccggcactgg agcacgtgat tcgaacgtgt ctggccaagg atccggacga gcgcttccag
     1021 acggcgcacg acgtaaaact gcaattgaag tggatcgcgg cgggtgcgcc gcagcttgcc
     1081 atgcctgcga tgcgctcgaa ccgcgagcgc ggcatttgga tttttgcagt ggtcacgctc
     1141 ctggtggcga tggctgcagt ttatttccac acgtcttcgg agcggacgca aacaacgtgg
     1201 tcttccattc tggccccgga gaacacgcgc ttcgcctact tcgccggacc ggtgacggtc
     1261 tcgcacgacg ggcgcaaact ggcttttgtg gccacgactt ccgcgggtca ggatgtggtg
     1321 tgggtgcggc cgctgggtgc gctaaacgct caggctttgc cgggcaccga agctgcctcc
     1381 tttcctttct ggtcggggga tgatcggtcc atcgggttct tcgcgagagg aaagctcaaa
     1441 accatcgaag ctgcgggtgg gccggtggtc accatctgtg acgctcccgg cgctcggggc
     1501 ggcacctgga accaaagcgg cgtcatcctg ttcgccggga cgtggggtgg aatctatcgt
     1561 gtcccaagtt ctggtggtac gccctctgag attacgaagc tggaccgctc acatggcgag
     1621 ctcacccacc gctggccgtt tttcctgcct gacggacggc acttctttta cctggccgct
     1681 aacttcatga gcggcaataa ggaggccaat tcagtttcgc tagggtcgct ggattccacg
     1741 gagaccaagc tcctgtttca cgcccgttcc aatgcagcct acacgtccgg ttatgttctg
     1801 tatgttcgcg accggacgct catggcgcaa ggctttgacg cgaagcaact ggagctgcga
     1861 ggccagcccc ttccgatcgc cgggcaggtg cagttcgacg agctcaccgg gagaggggtt
     1921 ttttcgggcg ctgccaatgg gactctggcc tatcagggag agaacaccgg ggccaactcg
     1981 cgcatggtca tgtttgaccg cgcggggaag gaagtcaaaa ccatcgggac gccggcggat
     2041 ttcgtccacc acaagatttc tcctgacggg caaagactgg cggtcaccgt gctggacccc
     2101 agcgtcgtga actacaagct gtggctctat gacctgttcc gggaaaaaga gacacgactt
     2161 acctttgggc ccgaccgaat tactaacccg gtctgggcgc cggagggaaa caccctcgtg
     2221 tttgggtcca acaagagggg gccttccgac attttcgaaa agcgcgcgga cagcgcaggc
     2281 agtgaagagc tcgtgctgca atccgacgcc aacaaatacc cgaccgactg gtcggcggac
     2341 ggacgcttta tcgcctacag cgtcagtgta ggcaaatcca agatcgaggt gtggattctg
     2401 cctcgcttcg gagagcgcaa gccctacatt tttctgcagg gtgatttcaa catgggagaa
     2461 gggcgctttt ctcccgacgg acgctggctg gcctatactt cggacgagtc gggccgggcc
     2521 gaggtttatg tcacaccctt cccctccggt gcgagcaagt ggcaggtttc cgtggccggc
     2581 ggcacgaatc ccaggtggcg gcgtgatggc aaagaactct tctatttggc ggccgattcg
     2641 gaattgaccg cggcgcaagt ggacaccgcc ggttccgtat ttcaggttgg cgccgtacgt
     2701 ccgctcttcc atatgctcct gagaacgggg ccgtcgcggc tggaattgag tcccacgtcc
     2761 gggcagatcg gctacgacgc tgcgcccgat ggaaagtggt ttgtggtgaa ttcgcctctc
     2821 gcgggcagcc ctccaccgat taccctggtc accaactggg cagcggagtt gaagaagcag
     2881 taacgctgga cgcatctcat ccaaacagac tgaatcctcc accgaagagc ctcccggccg
     2941 ttgacaaacg ctcattcgtc gtgacaggta agcgtgcaaa ggaggcccta tgagacgcac
     3001 ctctgtattc gtgactatcg cgtttctcgc aggactcggc gtcggcttct tcgccggaag
     3061 cgcctatcca gggatgcttc aacgaaggac gcatggcgca gacctggcgg ccatcgaaaa
     3121 actcgaaaga gcagacgttg aggccacact gacgcaagac ccgagcttgc tgattaatct
     3181 ctggtcggac gacggtgtaa aactcgacgt tcccggccca cctgtagtcg gaaagaaagc
     3241 catccaggag ctataccaga aagcccggac cgtctacccg gaattcaagg tgttgaaata
     3301 cgcgcccgac ttcaaagatg tccaaatcgc ggatggctgg gcgcttgagt ggggatattt
     3361 tgaagccatg tacaagatgt ctgcaaagga caatccggtc aatgtcaaag gcaaaggcgc
     3421 gcgtgtactg aagcggcaga gcgatggttc atggaaactc gccgtggtcg gcctgaaata
     3481 actgaaggga gagcggcgag cagggaccga atctgcgcgc ggtattctcc accctacgcc
     3541 gcggtcgtgg cggaatcctc ttcctgtacc gtggcttcca actccgacag cacggcgcgc
     3601 agcgcggtga tcaactcgat gcgcgagacc tggctcttct gaaactggag acgcaggcgg
     3661 aactgtcgcg tgtcgggctc gaactggaag atgaacggct gcggccgcgg cccggcggac
     3721 ttctcatcgc gccgctcggc gcgagcctgt tcgcggttga cgccgccgcg ggcgatgcgc
     3781 tcgaccatct ccagcatctt gcgctcgctg ggctggcgcg ccacttgcag cagcagcgac
     3841 ttcgacagga tgccgtgctc caggcatttc tggcgggccg cgccggtgat ggcgcagagc
     3901 gagagcgtct cggtgaccga ggagcgcgcg cggctgattt ttttggcaat gtcttcgtgg
     3961 gtgtagtcga actgttgggc gaggcgcgcc agcccttcgg cctcttcgaa cgcggtgagg
     4021 tctttgcgct ggaggttttc gatcagcgcg atctccaggg tctcggcatc gtcggcaatt
     4081 ttctcgatgc acgggacctc gcggagaccg gcggcgcgcg cggcgtgata gcggcgctcg
     4141 ccgctgatga tgtggtagcc gtcttcgcgc ggcacgtagc gcaccaggag cggctcgagc
     4201 acgcctttct cgcgaatcga ttcggtcagc tcgcgcaact cgcccagggc cttgcgcggc
     4261 tgctccgggt tgggctggat cttctcgatg gggatcatgc gccccaccgc cgcgccgctg
     4321 tagctggtca gtgattctac gtagtgggcg tcatgccgca tcttcagcgt gacgggcaga
     4381 ccgatccgtt tcgacacgat ggatgacctc cagagccagt ttgcgatact cgacggcgcc
     4441 gggcgatttc ggcgcaaact tcagaatcgt ctccttgtag gcgggactct cctcgaggcg
     4501 gacgttcttg gtaattttgg ttttgaacag cgtggggccg aacgtggcgc gaatctgttc
     4561 gtgcgtgtcg cgcgagatgt tggtgcgctt gtcgtacagg gtgatgagca cgccgaggat
     4621 tttcaggtcg gggttgggcc gcgcgcgaat gcgcgaatag gtttcgagca gatcgtcggt
     4681 gccttcaatc gcgaagtagg ccgcctggat gggcaccagc agatgcgacg ccgccaccag
     4741 agcgtttacg gtgaggatgc ccagcgcggg gggcgtgtcc agcaccacgt agtcgtagtc
     4801 cttgagcacc ggcgagagcg cgtccttcag cttgaagggt gcgtcgaact gaccggcgag
     4861 ctgcacttcg aggcgggcaa gggagagcct ccccggcgcc atttttagat tggcgtcctt
     4921 ggtcggacgg atgacgctgg ccaggctggt gcgctgttcg cccagcactt cgtgcatggt
     4981 ggtgtggatt tcgtcctggt tgaaaaacgc cagcgtggaa ttggcctgcg ggtcgaggtc
     5041 gatgagcagc gtgcgtttct tgcgttcagc gagagcggcg gcgagattga tggcggtcgt
     5101 cgtcttcccg acgccgccct tctgattcgc gatggcgaga acccaggtca actcccctgt
     5161 cgctccttct tcccggccgc ttgccgagcg ctatgagcgc cctggcagag ccgacccggc
     5221 aaccggaacc ccatacctag atgcccctgc ccggacccct actacgggta ggaatgtaca
     5281 ggggttagga gcctggcgca agcaaaaatt gcgacctcga ggaaatccgt ggtgggacag
     5341 gcccgctgcg gcggcggata tgtcccacta cgccgcccgc cgccctgccc ttgcctcacc
     5401 actttaggta caatgcacac cgctcggctc caacgcatga atgccacaac ggattgccgc
     5461 atcgtgacta tcgcttgccg cttcccgctc gccgcagttc tgttcctggc cgtgttcgca
     5521 cctgccgccg cgcagcaacc gccagccccg tccgcctcag ccgcggtttc tccagccacg
     5581 gcgccgaccg ccgcgtactc cgaaagctcc tccgggctcg agaagttgat gaaagatatt
     5641 ttgcgagcta ccaaggatgg cgatacggtg aagtcccgcg cgctgctgag cagcctggcg
     5701 cttccggacc ccgaggcctg gtttcgtggc gtattcggca gcgacgcccc cccgatgctg
     5761 agggagcacc cgcaatcccc gcccgtcatg gagagggcat tgatccagct tttctccaag
     5821 ttgctcaagg agaaattcac cgacgtgcgt gtgcagaagc acgaaaaaac atgcgacgac
     5881 aattcgggcg agcaaatcta cccggtgctc gttatgcgcc aatcgcccgt acccctctac
     5941 gaagtgcgct tccagagcgg cacgaacttc tatcgtctat gggcggtcgc ctatgcagat
     6001 ggcggatttc gttttgccgg ccatctccac gtgccggagt ctttctttcc cccggaaatc
     6061 gccggcgggc agtcggtgaa gagcatcata aagagaatca gcgtcgccgg aaacgtggca
     6121 aaagccaagc tcgtaaggca ggtgacgcca cgctatcctg aagccgcgaa gcaggaacac
     6181 cttcaaggca cggtaagact gcacgcgatc attcagaagg acgggtcaat ccggcagttg
     6241 cgcgtgttga ccggctactg ctctctgtcc gaggcctcga tcgaggccgt gcgtcagtgg
     6301 cgctatcgcc cgaccctgct caacggggag cccgtggaag tggacaccat catcgaggtg
     6361 attttccagt tgcgatcgtg agcgatcttc cccttctcgc tggacgcttc ccacgcggca
     6421 cgtttactcc gcccgcgtcc catcgggcac cggcgcttcc ggaacggcca gcacgggctt
     6481 gacgccatcg gcgtcgccga tgtcgaagag catgccctcg gagacgacgc cgcgcatttt
     6541 gcggggctcg aggttgacga cgaaaagcgc ctgacgcccg acgattttct gcgggttggc
     6601 gcgctcctgc ttcatgcctg ccacaatcgt gcgcgtgtga tcgccgaagt tcacgcgcat
     6661 ctgcagcagc ttgtccgctt ccggcacatc gtcgaccgcc tcgatggtgc ccacgcggat
     6721 atccacctgg ttcaagattt ccatggatat tgccggcttg aggggagcag gcgtcatgat
     6781 tgaagaccgc ctttgccagg ataggaacgc cgcggaagag attagtcaaa aatggaacaa
     6841 gccgccaggc gtgagaacga cggatgaagc ctctaaatcg gcaagcacaa tatcggcgtc
     6901 gaagttaaat ctccagtaat cccagttcgg atcccaagca gccccattgg tcccgcggag
     6961 gcttcgttca aacacctatt tttacaaatg aaaccccact tgccccgaaa tcctcatccg
     7021 gaaaactggt gacacgcttc aaactgatcc actttcctac tttgaggtca gtcgctgtct
     7081 ttatctttct tgcgatcatc atcctcatgg tcatcgtctt tcgggcggtg ttttttgtac
     7141 cagcctttgt gtttgccgtt atcgtgacgc tcctggcagc cccaattctc cggcccgcga
     7201 ttatcctctt ctttgcaatc agcattatgc tgatggtgct ggtaatacca atcgctctgc
     7261 cggttatggc cggcatatcc atcctgataa ccgagcttga atgcattcct ataatctcta
     7321 ctgatggccg ccacccctgc cgtggtgacc agggcagcgt tgtaattgga ggcgcgccgg
     7381 gcttggaaat cggcctggcc agctcgaaag ccctgatcga acgcctggcg gtagtagctt
     7441 cctcggggat cattgggcgg gtaattttga cccaagacag ggattgccat gacggcaaac
     7501 gtaacgcaga acagaaatat cgagcttgcg gtttttgatg aatacttttt gtgcatagat
     7561 ttacctccac cttcagaact gtcatttaca tcatcgtgga ttcctgccgg gcttcgtgtc
     7621 ccgaacttac gcttattcat aaggccagag tcgtatccgg gcaaatgtaa atgtcccgcc
     7681 gttcgcgcat caaatccaga cgagcgatcg gcttccggcg gttacatctt gtagcggccg
     7741 aggtcttcgt cgttgaggcc ctcgagccac ttgcgcagct cttcgttggt ggttttttcg
     7801 gtgacggcgc tggcgacctt ggaatttttc agcacttcgt catcgacgaa gatggggcaa
     7861 tcgaggcgca aagccagagc gagcgcgtcg ctggggcgcg aatccatgga catcagcttc
     7921 ccttcccgct ccatccagat gagcgcgaaa aaagtgtcgt ccttgagatc gctgaccacc
     7981 actttgtgaa cgcgcacttc gagacccgtc aggagatttt tcagcaggtc gtgggtcatg
     8041 gggcgcggcg tctggacctt ttcaatttcc agggcgatgg cgttggcttc gtaaatgccg
     8101 acccagatgg gaagaatggc ggtgccctgc gcgtccttga gcaccacgat gggcatgttg
     8161 gtgaccgggt ccatcatcaa gccccggatt ttcatctcca cttccatcgc gcctcccctg
     8221 gaagctccga cgacgcagaa aactcagacc acctgctccc ccaccaggct gttggggccg
     8281 gcgcgggtga tacggactgg cagatattct cccatcaggg cggtgcgcgg cgaagtgaaa
     8341 tttaccacgc ggttgctgga ggtgcgaccc gaccactgcg cttccttgtg accgcgattg
     8401 tcggtcagga cttcgaagct gctgccgacc agttgttcgt tgcgcagcat ttggatctgc
     8461 cgctggcggt cgaggagcac ggcgaggcgg cggtctttct cggcttccgg gattccgtcc
     8521 tgcatggcca gcgcgggcgt gttggggcgc ggagaaaatt tgaaggcgaa gacgccgtcg
     8581 tattgcgcgg cgtcgagaag cgtaagagtc tgctgaaaat cttcttccgt ctccccgggg
     8641 aaccccacga tgatgtcggt ggtcacgctg atggcgcggc gggcggcgcg gatcatggcg
     8701 attttgccga gatattcctc gcgggtgtag gtgcgcagca ttttttcgag caggcgcgtg
     8761 gagccggact gcacgggaag atggatgtgg tcgcagagct gcggatgcgt gtcgatggcg
     8821 tcgacaattt cgcgatggaa atcgcgcggg tgcgacgtgg tgaagcgcac gcggcggatg
     8881 ccccgcacct gggccacggc ggtgagcaat tcggcgaacg tcactttgcg cggcgaggga
     8941 tcgcgatagg agttcacggt ctggccgagg agctgaatct cgctgtagcc gatgtcggcg
     9001 aggtcgcgca cttcgcggag aacccggtcg ctcgggcggc tgcgctcggg gccgcgcgtg
     9061 aagggcacga cgcaataggc gcaggccttg tcgcagcctt cgatgatggt caggtaggcg
     9121 cggaagggat tgtcgcggcg ggtgatttcg gtttcgaaag tttcgtcggt gtcggtgtcg
     9181 aggcccatca cgcggcggtt gcccgattcg agctgcgcga gcagctcggg aagcttgcgg
     9241 tagctggccg agccgcagac caggctgacc cacggcgcgc gttcgaaaat ctcttcgcct
     9301 tcctgctgcg ccacgcagcc cagcacgccg acgattttgc gcgtgccggg cgcgccgccc
     9361 agatatttaa tggggatatg cctgcccggc gtcgcatcgg caggcgcgac gtccgggtcg
     9421 ccggtgggcg cgttgttggg attgaaaaga ccaaggcgtg aaaaaacttt ctgcgccgcc
     9481 ttttcgcgga tgctgcaggt gttgtagaac accaggtccg ccgccgcggg gtcgtttacc
     9541 atgcgatacc cgcgcgacat gagcacgccc gaaaccttct ccgagtcgtg ctcattcatc
     9601 tggcagccga aggtttcgat gtagaacgct ttgccttgtg tagcgctgcc ctttagggca
     9661 gcatcttcct cctgcttgcg tcgtgtcgcc tcgcccatga gggcggcatt tgtcgccgcc
     9721 gccagagaca cccctctgcc aatcaacgca tcggcacgaa tatcaaacgg ctggctcatt
     9781 cttcgaaatt tcagtctatc acatcgaaac aagctcacgg cggagcaccg gtttccgata
     9841 gtgggtcggt tcccggttag ccggctaacc aggatttagc caatgttaga ggtcggttca
     9901 ttgcttcggg caacgggaat caagaaattc acacacctct tgggatggat aatattcgcg
     9961 cgcggggccg aaggcaaatc cacgcagctt ttcgagtcgg ctatcggact gaatttccct
    10021 gaaggaaaag tagtcattcc ctgcaatctc gggtggccac agaacgcggg atttccattc
    10081 ggttgaatgg gaaagcccga gcttgacccg cacatcccat ttttgggaaa ggtgctcgac
    10141 tatcatttct cgcccatccg aatagaaact tgcggtggcg aagtactgtt tacctttctc
    10201 aattgacaca aagactagaa aatagtcatc caagaatggc ccttcgttcg ttgtgtactc
    10261 ggccattagc acgatgctgt ctaacggaca ctgccaaagc aactgaccct cagaatttct
    10321 atgttctaag gcggtgtgtt ccacattcag tactagctgg gacatggact gcatgaatcc
    10381 gccgactcca tctccgcgct ttgtcaatgg cgcgggctat caattcgacg atttgccgca
    10441 cttcgcagag tataagccaa tccaactgag tcagatcctt ttctctacgg atccactaga
    10501 cagctcaact cctgatagac gcgcttatcg gggactggct gctacccggc ttcttgaggg
    10561 gcgtaggacg cggggccgag catgcgcggc accattgacc tcctactgcc ccgcctgtat
    10621 catcgcgcgt tccaaactca atgcgagttc caaacacaat tcttcaggag aggatcacca
    10681 tgtcgaagcg tgcgagagtc tttgttacga aaatcaccgt tacgaaattc gctgttgcga
    10741 aatacattgt aatgatcgga gtggccgcgc tgttggcggt cgcgctgttg gccacgccgg
    10801 gtttgatgca agcgcagcag gcgcagggca attctcaagc cgggtctcaa gtcgggcccg
    10861 acgtgtacgg gcagatcaaa taccgataca tcgggccgga aggcaaccgc gcgacatccg
    10921 ttgccggcgt gccggggaat cccaacatct attatgtggg cgcggcttcg ggcggaattt
    10981 tcaagagcag cgacggcggc atccactggg agcccatctt cgacggccag ccggtggctt
    11041 ccatcggatc gctggcggtg gcggcgacgg acgcgaacat tgtctgggcg ggaaccggcg
    11101 aatcattcat ccgcagtcac atctccgtgg gcgcggggat ttacaaatcc accgacgcgg
    11161 gcaagaactg gacgttgatg ggcctcgaaa aggccgggcg catcggccgc gtggagatcg
    11221 acccgcggaa tccggacgtg gtcctggcct gcgcgctggg tcacgcctac gggccgcagc
    11281 cggagcgcgg cgtcttccgc accaccgatg gcggaaaaac ctgggagcgc gtgttgtttg
    11341 tggacgagaa caccggctgc tccgatatgg gcatggaccc caacaactcg cgcatccttt
    11401 tcgccggcat gtggcagatc gagattcaca cctggggccg caccagcggc gggccgggca
    11461 gcggattgtt caaatccacc gacggcggcg cgacatggaa gcgactggag gggcatggcc
    11521 tgccgcattc gccggtgggc agaattgcgg tgcgcgtggc caagaaagat tcgagtcgcg
    11581 tgtacgcgga gatcgaaacc ggcgacggcg tcccgcagcc gaacagcccc gggcagctcg
    11641 gccaactgtg gcggtccgac gacggcggcg agaactggca gatggtcaac tccgaccggc
    11701 agctccgcgg gcgcacgcac tactacacgc gcctggaaat cgccccggac aatgagaatg
    11761 aagccttttt cttctccggg tcattttcga gaacgctgga cggcggccat tcgctcacca
    11821 agatgcccgc cagccccggc ggcgacttcc atgagatgtg gatcgatccc gcgaacggcg
    11881 atcgcatggc ggtggtctgg gacggcggcg taagcatctc cgtaaaccgc gggcactcct
    11941 ggaactccgt acagttt
//
LOCUS       DASYVO010000415         8252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_277765, whole genome shotgun sequence.
ACCESSION   DASYVO010000415 DASYVO010000000
VERSION     DASYVO010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277765"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VGR03_13870"
     CDS             complement(<1..522)
                     /locus_tag="VGR03_13870"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1995413.1"
                     /translation="MLIGLFPELDAPGGVQRAGRHLAAVLTEFASSRGMDCRLLSLND
                     SPELHRMTLGGREFVFTGCDRAKGRFTVTALRAARRKAKLVLAAHPNLGPVVQAMRIA
                     APRMKSIICTHGIDVWEPLGVLRRRALRHANLVLAPSRDTAEHVASEQGVARERIRVL
                     PWALDPQFEGLLAA"
     gene            complement(650..1507)
                     /locus_tag="VGR03_13875"
     CDS             complement(650..1507)
                     /locus_tag="VGR03_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995414.1"
                     /translation="MNSSTKYIAAFICLALPGIAQAQSVGLVECPRTGDYVYLYSSMT
                     TMDVRTTLQCGEQLQITGRYDRYFGVRTAKGEIGFVPVESLLLLKDKPGAKAPPPKTA
                     QPTTRERTPYDAPAPQTEVAPSAPLSPSEFTLRNGTPIHLKLGKTLSSATAHVGDVVD
                     LQVVEEVIVDGFSVIPQGTAAVGLVTDAEPKKRMGHGGKLAFSINFVRLKDNEKAAVR
                     SFQESSGSNSSAGAILSLSSGKEVVFTQGTEFTAYVDGDMQLKREAFQLEKEPPAPPA
                     RKPSQPRRL"
     gene            complement(1694..2365)
                     /locus_tag="VGR03_13880"
     CDS             complement(1694..2365)
                     /locus_tag="VGR03_13880"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEV1995415.1"
                     /translation="MTAPNFSSVRALIFDLDGTLIDSQRDLILSVNAMLREMGREHLH
                     EDTISGYIGHGAPQLVGRALGDGSTEEERQRALKFFLAHYEEHKLDSTCAYPGVPEAL
                     EHLAAFSMAILTNKPVRVSVRILEGLGLAKYFRAVYGGNSFETKKPDPLGAQAILREF
                     GAAPAEAILIGDSEVDVQTARNAGTLAAAVNYGFGTHDRAAHPADIYLDRLTDLVPLL
                     IGRNS"
     gene            2523..3896
                     /locus_tag="VGR03_13885"
     CDS             2523..3896
                     /locus_tag="VGR03_13885"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEV1995416.1"
                     /translation="MAQVYPFRAFRYNPARAPFDRVLTQPYDKISPAMQEKYYAADPH
                     NLIAVEKGRAYTGDTPQNNVYTRAAAAIEDWTRSNVVVQDPAPSFYTYTQEYTVPGTV
                     DRYAVAQDEVRRTRRGFIGAGKLEEYSAGVVFRHEHTLSGPKADRLELLRHTKTHTGQ
                     LFMLYSDAQRRIDGILAEAESAAAPATEMRDEYGVLHRLWVIAEPQRVAAIAKAMEDQ
                     KLVIADGHHRYETALNYRNECRTRAGKIDPEAPYERVMMTFVNTCSEGLMILPGHRVA
                     AHVHDFSWSGVRRYLEPWFAAEEFPFSGSGERAEAKKKFLAKLTGARTKRAIGVYPAP
                     GEQKRAFYVLTLREGVNLAQLLPNVSPLQRELDVVLLHEGILEPALGITPQAVTAEAN
                     LTYEREATAALDAVDCGRAQISFLLNPCDVEQVMKIATAGEVMPQKSTDFYPKLLSGI
                     TIYRVES"
     gene            4443..5819
                     /locus_tag="VGR03_13890"
     CDS             4443..5819
                     /locus_tag="VGR03_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995417.1"
                     /translation="MEKSKALGGKPGTWSTLPSFGTWHHRPGNLPRPCYNYPIRASVM
                     KVAVSQFGVDPEPEHSEITRRLLGSSAIVSHNPDQRDISVALDVGGFVRRLLLFDTYI
                     LYSVRLKEIPELVRHFGFQGTMDLLSSGALEIRCECAQYAEGTLNTPPCPPLTFQFHV
                     LEAHIWEQYLIDSLPALRNAPLSGRELMDLQGAVVKAVRRSDNRRMFSSEVAPAFEGE
                     LLSNERLVKAAVTLVLSKQNGMHELDDFTLKIHKVGDDRFQAETNLDSKLRLGSQDIH
                     NTIKTALLGVAGLFQRMGEMKAHVAVAGFTEDELSLFRNKFESLVDALGSNRNEQRFL
                     RVISIAGLSEDFVPNQRIDIEKILEIRNEPEAMEFRAWLADIDRLSDSEIRERVASLN
                     AKLGLAVQTGTGKVLRLLVTTLAGILPPVGMAVSALDQFLWDRFFRRSGVAAFVNDLY
                     PSIFGKRT"
     gene            complement(6365..6664)
                     /locus_tag="VGR03_13895"
     CDS             complement(6365..6664)
                     /locus_tag="VGR03_13895"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV1995418.1"
                     /translation="MKKSKATVTRNAAELAKALGLTPADGAEIALRSDLNSKIVEVVQ
                     RKGLTHAQVARLARTSRTRLTAIMNRNTKDISTDLLLRVLYSLGYTAKIKFQKAA"
     gene            complement(6946..7683)
                     /locus_tag="VGR03_13900"
     CDS             complement(6946..7683)
                     /locus_tag="VGR03_13900"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEV1995419.1"
                     /translation="MPSFVFRNPRPAKSAVKESPKPVAKSFKAMLERMSSNLGWVIVR
                     IPLDVPKVWGTRGMLKVKGEINGFAFRSSLFPTGKGYHYLLVNKRMQAGANARAGSVA
                     QFRLEPDMEKRVATVPAELQRILNEERSFRRWFGQLNYSTRKWIADWVTQVKSAEARV
                     RRAEQVAEQLLATMEAERDLPPILKLAFARDPRAFAGWQRMSPSHRRGLLLAIFYYRT
                     PDARDRRIAKMLEDALAFADRKPKKQK"
     gene            complement(7759..>8252)
                     /locus_tag="VGR03_13905"
     CDS             complement(7759..>8252)
                     /locus_tag="VGR03_13905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV1995420.1"
                     /translation="SATYRGRRQPNGKNNRSRCGSLLIRGAVPGLVAAGLLRDEEETN
                     VALKMTNREVDGVAVVALDGRIVLGEESNALREKVKALMAEGKKKVVLNMDNITFIDS
                     AGLGTLVAAHHSVKTQGASLRLCHLGSKFQEVLQITKLLTVFDVYNSEAEAVSSFSKG
                     AGA"
BASE COUNT         1734 a         2204 c         2576 g         1738 t
ORIGIN      
        1 tgccgcgagc aacccttcga attgcggatc gagcgcccag ggcaaaacgc gaatccgttc
       61 gcgcgccact ccttgctcag acgcaacatg ttcggcggta tctcggcttg gcgccagaac
      121 caggtttgcg tgacgcagcg cgcgccggcg caagacccct agcggctccc agacgtcaat
      181 cccatgcgtg cagataatgc tcttcatccg cggcgccgca atccgcatcg cctgcaccac
      241 gggtcccaaa ttcggatgcg cggccaggac cagttttgct ttgcgccgcg ctgctcgcag
      301 tgctgtcacc gtgaatcgcc ccttggcgcg gtcgcacccc gtgaacacga attcacgccc
      361 tcccagtgtc atccggtgca actccggcga atcattcaag ctgagcaggc ggcaatccat
      421 gccgcggctg gaggcgaatt ccgtgagcac cgctgccagg tggcggcccg ctcgttgcac
      481 cccgccgggc gcgtctagtt caggaaagag gccaatgagc acgggcaaag tctgccaagt
      541 gcggcgcaca atggcaagtg cggatgcatt gcggtgtgag aaaagcctgg ctcggtcgcc
      601 gagcttccgg gaggcaagat cgcggatgcg ctgctgtcta tctaaagctt caaagcctgc
      661 gcggctgaga gggcttgcga gcgggtggtg cgggaggttc tttttcgagc tgaaaagctt
      721 ctctcttcag ttgcatatcc ccgtcaacgt aggcggtgaa ttcggttcct tgggtaaata
      781 ccacttcttt gccggaagac aaggaaagaa tcgctcccgc tgaactgttt gagccggaac
      841 tctcctggaa ggagcgaacc gcggcctttt cgttgtcctt aagacgcaca aagttgatac
      901 tgaaagccag ctttccgccg tggcccatgc gttttttggg ttcggcatcc gtgaccaagc
      961 cgacggccgc tgtgccttgc ggaatcaccg aaaaaccatc cacgatgacc tcttccacga
     1021 cctgcagatc cactacgtcg ccaacgtggg cggtcgcgga agaaagggtc ttgcccagtt
     1081 tcaggtgaat cggagtgccg ttgcgcaagg tgaactcaga aggagaaagg ggagcgctcg
     1141 gagcaacttc cgtttgtggg gcgggggcgt cgtacggagt gcgctctcgc gtcgtgggct
     1201 gcgccgtctt tggcggcggc gccttggcgc ccggcttgtc tttcaacaag aggagactct
     1261 ccaccgggac gaaacctatc tcgcccttgg cggtgcgcac accgaagtag cggtcgtacc
     1321 gtccggtgat ctgtagctgc tctccacact ggagcgtcgt gcgcacatcc atggtggtca
     1381 tggaactgta tagatagacg tagtcacccg tgcgcgggca ttccaccaga cccacgctct
     1441 gcgcttgggc gatccctggc aacgccaggc aaataaatgc ggctatgtac ttggtgctcg
     1501 aattcattct gcacccgctg gtccgaaggg ttgaagatgc cacgctacta tctcagcgta
     1561 cgatggttcc tgctgacaag aaatgggact gccgcactgt gcataaaaag atacggtgga
     1621 gaaacaacct gacggcccgt gtccgctacg gatgcgcgtc cattttgtgt tgtcatggca
     1681 ctccgcgaga cgatcatgaa ttgcggccga tcagcagcgg gacgagatcc gtgaggcgat
     1741 cgagataaat gtcggcgggg tgggcggcgc ggtcgtgggt gccgaagccg taattcacgg
     1801 cggcggcgag agtgccggcg ttgcgcgcgg tctgcacgtc cacttcggaa tcaccgatca
     1861 ggatggcttc ggccggagcg gcgccgaatt cgcgaagaat cgcctgcgcg ccgagcggat
     1921 cgggcttttt cgtttcaaag ctgttcccgc catagacggc acggaaatat ttcgccagac
     1981 cgagtccttc cagaatgcgc acgctgacgc gcaccggctt gttggtgagg atcgccatgg
     2041 agaacgcggc gagatgctcg agcgcttccg gaacacccgg ataagcgcag gtcgaatcca
     2101 atttgtgctc ttcgtagtgc gcgaggaaaa actttaacgc gcgctgacgc tcctcttccg
     2161 tgctgccatc gcctaatgca cgaccaacaa gttgcggcgc gccgtggccg atgtagccgg
     2221 agatggtgtc ttcgtgcaga tgctcgcggc ccatttcccg cagcatggcg ttgacggaga
     2281 ggatcaggtc gcgttgagaa tcgatgagcg tgccgtccag atcgaaaatc aacgcgcgca
     2341 cggaagaaaa attcggggca gtcatgaacc ccgtcattgt agtatgaccg cactttttcg
     2401 acagtcatca atctcacgcc gaatttccac cgaaaattaa ccttgcgtct ttttcggaat
     2461 tccggcgtgt tcgcgtaaac tagagggatt cgctggcggt ataaaacgat tccggagact
     2521 ccatggccca ggtttacccc tttcgcgctt ttcgctacaa cccggcgcgc gcgccgttcg
     2581 accgcgtcct gacgcagcct tacgacaaga tttctcccgc gatgcaggaa aagtattacg
     2641 ccgcggaccc gcataacttg attgccgtgg agaagggccg cgcgtacacc ggcgacacgc
     2701 cgcagaataa tgtttacacg cgcgcggccg cggcgatcga ggactggact cgcagcaacg
     2761 tggtggtgca agatccggct ccgtcttttt atacctacac gcaggaatac actgtgcccg
     2821 gcacggtcga ccgctatgcg gtcgcccaag atgaagttcg ccgcacgcgc cgcggattta
     2881 tcggcgcggg aaagctcgag gagtattccg cgggcgtcgt cttccggcac gagcacacgc
     2941 tttccgggcc gaaggcggac cgcctggaac ttctgcggca cacgaagacg cacacgggcc
     3001 agcttttcat gctgtattcg gatgcgcagc ggcgcatcga tggaattctg gcggaagccg
     3061 aatcggccgc ggcgccggcc accgaaatgc gcgacgaata cggcgtgttg caccggctgt
     3121 gggtgattgc ggagccgcag cgcgtcgccg cgattgcgaa agcgatggag gaccagaagc
     3181 tcgtgatcgc cgacgggcat catcgttacg agacggcgct gaactaccgc aacgagtgcc
     3241 gcacgcgcgc cggaaagatc gacccggagg cgccgtacga gcgggtgatg atgacgtttg
     3301 tcaatacgtg cagcgagggg ctgatgattc tgccggggca ccgcgtggcg gcgcacgttc
     3361 acgatttttc ctggagcggc gtgcggcggt atctcgaacc atggttcgcg gcggaggaat
     3421 tcccgttctc gggcagcggg gagagggccg aagcgaaaaa gaaatttctt gcgaaactca
     3481 ccggcgcgcg cacgaaacgg gccatcggcg tttatcccgc tccgggggag cagaagcgcg
     3541 cgttctacgt gctgacgttg cgcgaaggcg tcaacctcgc gcaactcctg cccaatgttt
     3601 cgccgctgca gcgcgaactg gacgtggtgc tgctgcacga aggaatcctg gagcccgcgc
     3661 tgggcatcac gccgcaagcg gtgactgcgg aggcgaacct gacgtatgaa cgggaagcca
     3721 cggcggcgct cgacgccgtg gactgcgggc gcgcgcagat ttcgttcctg ttgaatccct
     3781 gtgacgtcga gcaagtgatg aaaatcgcga cggccggcga agtgatgccc cagaagtcca
     3841 ccgattttta tcccaagctc ctgagcggaa tcacgatata tcgagtggag agctaaggaa
     3901 gaaagagcga agcaacgatg taatgaagta acgaggtaaa gaaaagggag atcccgcgag
     3961 tccatccggc cgtccaagac agattccctt ctcaagaatg aagagagagc cctcgctccc
     4021 gatgttcgct cgggacagac acccttcatc gggtgtctgg ttaacaataa caccccccac
     4081 cccctgtttt tcgtaagtgt ggcaactaaa gggtttaggt ttcctgtaaa tcctttgttt
     4141 gccacactta cgggatagtc tgtaagtgtg gcatgcaaag gggttacggc tatgaaaatt
     4201 tgaagattcg agggctgtac tggttggaac tgaaagaggc ggacgaggtg cccgccggtg
     4261 agaagggcac tctggggctt ggcaaacacg ggagagaata gcatgccggt atcaactggc
     4321 taagtagaaa gtggggaacg gtagaaaata agggttgaat ggttgtttag gcgggttgaa
     4381 cggttttggg ggagggaaag ggtttgcggg aggggtggga ttcgaaagaa ttgtggaaat
     4441 ccgtggaaaa atcaaaagcc ctgggtggta aacccggcac ctggagcacc ctgcctagct
     4501 ttggcacctg gcaccaccgt ccgggcaacc taccccggcc ctgctataat tatccaatta
     4561 gggcaagcgt aatgaaggtt gctgtatctc aatttggtgt agatcccgag ccggaacact
     4621 cggaaatcac gaggcgacta ctcggctcgt cggcgatcgt ttctcacaat ccagatcaaa
     4681 gggacatatc cgtagcactt gatgtgggtg gctttgtgcg acgattactt ctatttgata
     4741 cgtacattct ctattccgtg cgcctgaaag agattccaga acttgtacgc cactttggat
     4801 ttcaaggaac aatggatttg ctttcaagcg gtgctttgga aatccgctgc gaatgtgccc
     4861 agtatgcgga gggcaccctt aacaccccgc cctgtccacc cttgaccttt cagtttcatg
     4921 ttcttgaggc gcacatttgg gagcagtatt taatcgatag tttgcccgca ctgagaaatg
     4981 cgcctttgag tgggcgcgag ctaatggatt tgcagggcgc cgtcgtcaaa gctgtcaggc
     5041 gcagcgacaa taggcggatg ttttcttccg aagtagcacc agcttttgaa ggcgagctcc
     5101 tttccaacga gagattggta aaggctgcgg tcacgttggt cctttctaaa caaaatggca
     5161 tgcatgaact cgacgacttt accttgaaga ttcataaagt tggagacgac agattccagg
     5221 ctgaaacaaa tctggattct aagcttcgcc tgggttctca ggacattcac aacacaatca
     5281 agaccgcgct acttggagtc gcggggttgt tccagcgcat gggagagatg aaggcacacg
     5341 ttgcggtcgc aggcttcacg gaggacgaac tatctctatt ccgaaacaaa tttgagtcat
     5401 tagttgatgc cttggggtca aatagaaatg agcagcgctt cctgagggta atctcaatag
     5461 ctggactatc tgaggatttc gtacccaatc aacgtatcga tatcgagaaa atcttggaaa
     5521 ttcggaacga acccgaggcc atggaatttc gcgcttggtt agccgacatc gacagactta
     5581 gcgactctga gataagggag cgcgttgcga gtctcaacgc gaagcttggt ttggccgtcc
     5641 agacggggac aggcaaggtg cttcgactct tagtaacgac tctggctggt attttgccgc
     5701 cggtgggaat ggcagtgagc gcactcgatc agttcttgtg ggaccggttt tttaggaggt
     5761 ctggcgttgc tgccttcgtc aatgatctct acccctccat ctttgggaaa cgaacgtaga
     5821 gagttctctt aggcgcgtgg cgcaggctga atgggccaaa agaatttcag gcggcttttt
     5881 ggaatttgag tttggcggtg tagctgaggg agtaaaggac gcggggcggg gggacaaagg
     5941 aagtgattag tggttagttg tcagtggtta gtttcggagg gggaagtagg gtggcgagtc
     6001 ccgacccggt cgggatctac gctccataac catgatggag cttcgatgat gagtggcgag
     6061 ttctttgacc gagcgtgctt gcgactcttc aaggatggcg ttggggttcg cgacggcgcc
     6121 aatcgggaga ttggcgttcc cggaggcgtt cggctggacc tagcaggctg ctgaagaagt
     6181 cccattcccg tcattccgag gagctgcagg cgacgaggaa tctgtttttt tcttggctct
     6241 tctgcaaaga gcagatccct cgctacgctc gggatgacaa caagaagagt ttttcagcaa
     6301 cctgctaggt agagccccga tggaactagg acaggccgat cgcaggagca aagcccgggc
     6361 agagtcaggc ggctttttgg aatttgattt tggcggtgta gccgagggag tagaggacgc
     6421 ggaggaggag atcggtggaa atgtctttgg tgttgcggtt catgatggcg gtgaggcggg
     6481 tgcgcgaggt acgtgcgagg cgcgcgacct gagcatgcgt caagcctttg cgctgaacga
     6541 cttcgacaat tttcgagttg agatcgctgc gaagcgcgat ttccgctcca tccgccggag
     6601 tgaggcccag ggctttggca agctccgcgg cgttgcgtgt gacggtggct ttagacttct
     6661 tcatggagca actcctttag ccgcacccac gagtaggatg tctcatatct gggacatggt
     6721 gtcaagggag ggaggtcagt cgcctccccg agggggcggg gtaaacgcga gctgtgagag
     6781 tcgccaccgc gctgtcgagc aaagaaagat ggcgacgtga agtcgccgct acatgagccg
     6841 gactaaagtc cggcctctac ataagcgaag aacaaaatca ccggtctgaa gaccggccac
     6901 tacaagtgaa gacataaaac ggcgagccta gaaggctttt tgtgactatt tttgtttttt
     6961 tggttttcgg tcggcgaagg cgagggcgtc ttcgagcatt ttggcgatgc ggcggtcgcg
     7021 ggcatcgggg gtgcggtagt agaagatggc gagcaagagt ccgcggcggt gcgatgggga
     7081 catgcgctgc catcccgcga aggcgcgagg atctcgggca aaggcgagct tgagaatggg
     7141 cggcaggtcg cgttcggctt ccatggtggc cagcaactgt tcggcgacct gctcggcgcg
     7201 gcgcacgcgg gcttcggcgc tctttacctg cgtcacccaa tcggcgatcc acttgcgggt
     7261 ggagtagttg agttggccga accagcggcg gaaggagcgt tcttcgttga ggatgcgctg
     7321 gagttccgcg ggcacggtgg cgactcgttt ttccatgtcg ggctcgaggc gaaattgtgc
     7381 gacgcttccg gctctggcgt tggcgcccgc ttgcatgcgc ttattgacga gcaggtagtg
     7441 atagcccttg ccggtgggga agagcgaact gcgaaaggcg aagccgttga tctcgccttt
     7501 gaccttgagc atgccgcgcg tgccccagac tttcgggacg tcgagcggaa tgcgaacgat
     7561 aacccagccg agattggacg acatgcgttc aagcatggct ttgaaggatt tcgcgactgg
     7621 tttcggactt tccttcacgg cggatttagc gggacgagga tttcggaaga caaaggaggg
     7681 cacgccggag cgtgccctcc ggcaaagggc acacgcgcaa aaccagcgtg tgcggcactt
     7741 gccatctggc ttggacgctt atgctccggc gcctttggag aagctggaga cggcttcggc
     7801 ctcggagttg tacacatcga agacggtcag caacttggtg atttgcagga cttcctggaa
     7861 cttgctgccc aggtggcaaa ggcgcagaga agcgccctgc gtcttcacgc tatgatgagc
     7921 ggcgacgagc gtacccagcc cggcgctgtc gataaatgtg atgttgtcca tgttcaggac
     7981 gactttcttt ttgccttcgg ccatcagggc tttcactttt tcgcgcagcg cgttactttc
     8041 ctcgccgagc acaatgcggc cgtccagcgc cactacggcc acgccatcca cttcgcggtt
     8101 cgtcatcttc aaagccacgt ttgtttcctc ctcatcacga agtagtcccg cggcaaccaa
     8161 ccccggaaca gccccgcgta tcaacaacga cccgcaccgg ctgcgattat ttttaccgtt
     8221 aggctgccgc cggccccgat aggttgccga tt
//
LOCUS       DASYVO010000416        15055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_40035, whole genome shotgun sequence.
ACCESSION   DASYVO010000416 DASYVO010000000
VERSION     DASYVO010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 15055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15055
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40035"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1123
                     /locus_tag="VGR03_13910"
     CDS             212..1123
                     /locus_tag="VGR03_13910"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEV1995421.1"
                     /translation="MSESSPRRSLPKRIVLILGGLAVFTLVFVIAIRLAVVGIFSGIE
                     QNRATGLSAFSPFAHYSAASDLITLGNIWISRSAALQMCTASFDRSVEALHRIVASRH
                     GYLEDLRTESRSGSGRTLSANVSVPASDFEAVLSEVKTLGRIETISEAGEDSAVKSNS
                     ATRHLAAAQTNLSRLQKLQRARKGELRDAVALEKDIAQANEAVIEAERQSQGLVSTVA
                     QAHIRLVLLEDYRAPLQANLAGLFPQLRNSLVEGVSSIFSSAALVLGVLLEFGLPLLF
                     WAALLFVPARFAWRRFRRVPEVLPVSQ"
     gene            complement(1211..1549)
                     /locus_tag="VGR03_13915"
     CDS             complement(1211..1549)
                     /locus_tag="VGR03_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995422.1"
                     /translation="MSALSSGSGVAARLAPAKPKTHGFLHIVRHGGGPKAIYIVTYQR
                     LDHDGAPVKPVLAEGAQALIELLERIGVDFHLKEVRGALEDILRLGSANIPDLWLSDE
                     EMLEKGLVEG"
     gene            complement(1581..4382)
                     /locus_tag="VGR03_13920"
     CDS             complement(1581..4382)
                     /locus_tag="VGR03_13920"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEV1995423.1"
                     /translation="MATVKHALSGKSPATDAAAEKTASGAGKGLRFQRYFTDGKKSPF
                     DAVEWEKRTALIGNEKGVTIFRQEDVEVPKSWSQTATNIVTSKYFHGKPGTAQREGSV
                     RQLIGRVVNTIVRWGEECGYFADMPSRDAFRDELTHLLVEQKMAFNSPVWFNVGVQPK
                     PQCSACFINSVSDNMESIMGLTRTEGMLFKWGSGTGTNFSTLRGSRETLSGGGIASGP
                     VSFMKGFDAFAGVIKSGGKTRRAAKMVILNIDHPDIVEFIECKRKEERKAHVLIEQGY
                     DSSIDGDAYASIFFQNANHSVRVTDDFMRAVIEDKDWWTKNVLDGQPNEKLRARDLMM
                     KISDSTWHCGDPGMQYDTTVNRWHTSKNTARINASNPCSEYMFLDDTACNLASLNLMK
                     YVNASGQFDVAAFKHAVDVTITAQEILVDNASYPTPKIEENSHNFRPLGLGYANLGAL
                     LMSMALPYDSDEGRDVSGAITALMCGEAYAQSSRIAERMGPFPGYEVNREPMLDVMRM
                     HRDAMRGIQPEHVQTELFMAAQESWDTALAHGEKHGYKNSQVTVLAPTGTIGFMMDCD
                     TTGIEPDLALVKYKKLVGGGLIKIVNNTVPQALMKLGYTPEQTSEIVSYIDQNGKIEG
                     APHLKAEDLPVFDCSLAPQGGGRSITWTGHVKMMAAAQPFLSGAISKTINMPEESTVE
                     DVMNAYIESWKVGLKAVAVYRDNSKRSQPLNAGGKKEEKQAETAAVVEPMQRELFARR
                     QREKMPVERASVTHKFSVGGHEGYITVGMYDDGRPGEIFIKMSKEGSTLSGVMDGLAL
                     TISLGLQYGVPLKVFVDKLVNTRFEPSGITANANIRFVSSVLDYIARWLGGRFISADY
                     LKLNGAAATENAATAAPAAVLPAMTQANLDAMQTALSASRPSNAHEGAPTCSECGMLM
                     VPNGACYKCENCGSTSGCS"
     gene            4948..5607
                     /locus_tag="VGR03_13925"
     CDS             4948..5607
                     /locus_tag="VGR03_13925"
                     /inference="COORDINATES: protein motif:HMM:NF015142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HEV1995424.1"
                     /translation="MKRSLLPLAILLVLPLHLFAQTAAPATALPIAQSAFGQKLHISG
                     IPNAGRVTDALYRGAQPREVGFSELKILGITTIVDLRSEDREKIAWERKHAESLGMRF
                     VHIPVDGWSAPTNEQVVQFLSLLHDNPGQKIFVHCHFGEDRTSVFIAAYRIASDKWPV
                     EQAIREMYFFGFNGLWHPAMKSFVHDFPSRLNSAPALAPFRALPSQPSSAGSLVPPRP
                     S"
     gene            6209..7087
                     /locus_tag="VGR03_13930"
     CDS             6209..7087
                     /locus_tag="VGR03_13930"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV1995425.1"
                     /translation="MQVGRYEILEIIGTGAHGRVARAHDSMIGRLVAIKLFPKELASG
                     EARQRFIQEARVVGQLSHPSIITLHDMGIDEATQTPYLVMEFLEGQPLDRILEKGSLP
                     FPKACAWAAEVACALGVAHRKGVIHGDVKPANMLITDDGRVKLMDFGMARLASRDSGA
                     TPLLGTPAYWCPEQIMGKPQDARSDLFSLGVVLYEMVTGKRPFDADSLPAICGRVLSS
                     TPLPPSHANPSVPAGFDAVVARCLAKDPAARYATAEALAEELYPLARHKTAPVPVLHS
                     NGNGLRDRAARLLRSA"
     gene            7087..8301
                     /locus_tag="VGR03_13935"
     CDS             7087..8301
                     /locus_tag="VGR03_13935"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV1995426.1"
                     /translation="MITSQKGRYEALELIGSGTTSRVEKARDNVIGRTVALKTFINNF
                     DEHLEQQFLREAQLVGQLSHPAVVQLYDVGIDQHGTPFLVMEYVAGKTLEQHLEASTL
                     TIQRACAWAADLANALALAHRAGIIHGDVKPGNILVTPENKVKLGDFGIARLTTQLSA
                     SGQLMGTPAYLAPEQIQNEFQDQRSDQFSFGVVFYQMLTGIRPFAGNSLGAVCAQILN
                     DDPVPPSRHNLAVPPALDRVVARCLAKNPQDRFTSCEDLAKSLYPFTRSRPRTPEPVF
                     KKRSWWSAPAGHREVWMAASACLLLAAFVQIPRLLSARYGIPPAPTRQYFHPGVPYEA
                     FVYTRQTVAQAAAPDAPGDFDPRMADKHSSPRIIRVEKLALAQLNKPAISQNLSSDSS
                     SASARSPQSNTP"
     gene            complement(8384..9622)
                     /locus_tag="VGR03_13940"
     CDS             complement(8384..9622)
                     /locus_tag="VGR03_13940"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1995427.1"
                     /translation="MAQAHGENLKQAMDTLRAHKLRSFLTIFGVVLGVSVIMLVAALI
                     TGFDSQVQENIKQYGADTAFISRFDHGPHNGRRPKDERERKPLTLEDAQAIQERCPAV
                     KNVTVFLTWWEQQHTVRTKSGSVSAIDFRGVQPNFGQVYANAATLEGRFISEGDDLHR
                     EKVVLLGENAAPVLFPEGSPVGKDVMIDGSAFQVIGVVEKPKGMFGNDDEDRRVLIPY
                     NTFRKIYPGAYENQIRLQAFPNLLDQAVDQTTEVLRRRRNVPYDGKDNFSIQTSQQEV
                     EQFHSILGMVALATVVLSGIGLLIGGVGVMNIMLVSVTERTREIGIRKAIGAKSGDIT
                     WQFLLEAMALTGAGGVIALVLVNGLVFLVHVGLKWPGSVPLWAAVTGIVVSVCVGLIF
                     GVWPAMKAAKLDPVEALRYE"
     gene            complement(9630..10952)
                     /locus_tag="VGR03_13945"
     CDS             complement(9630..10952)
                     /locus_tag="VGR03_13945"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1995428.1"
                     /translation="MGVTPIGQVGAIPRVVPQGLDIAGKLGRLRSALVTMGETLRLAL
                     DSLRAHKLRSFLTLLGVILAVTTLVVVMSVVAGLNFYVADKVANLGANVYVISRFGII
                     TSEDAWIKAQKRPLITREEFGSLKEGMRTTSQIAALLGTSSDVRSGNTLQEDVNILGV
                     TANYIEVRNLSVAAGRFLMEADETHRSEVCFIGADLAKKFFGNVDPIGKTVRAGTHSY
                     EVIGVAAVIGSAFGQSQDNFVFIPLGTYIKEWGTQRDSLLLFAQARNAELIPASEDEA
                     RMLLRAWRHIPWEADDSFGVVGSESIMGLWKQLTGNLFAVAVGLTAVFLVVGGIVIMN
                     IMLASVTERTREIGLRRSLGARRKHIVLQFVTESAVLAATGGVMGIALAYAMVAAARA
                     ATSIPMRTPMSAVILSLCVSTAVGLFFGIYPAMRAAKLDPIEALRADG"
     gene            11192..11815
                     /locus_tag="VGR03_13950"
     CDS             11192..11815
                     /locus_tag="VGR03_13950"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV1995429.1"
                     /translation="MRRICLYFLVLTVFGLNLMPSSQAQDTLDHKTPAMASAAPTSGF
                     RAEFLEEITYYEQRYTRLAEAMPAEKYVWRPAEDVRSVGEVFTHITAANYGIARALGT
                     APPASFDPKAITALSADKPKVLQALKDSFAHFRGAIIAFNEADGDKPQNIFNRHTTLR
                     GSFIAITGHFGEHLGQSIAYARVNGVVPPWTEDFQKQQQKPADKPKP"
     gene            11951..12868
                     /locus_tag="VGR03_13955"
     CDS             11951..12868
                     /locus_tag="VGR03_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995430.1"
                     /translation="MMLKRTFITIALLSLVLAADAAAQSVVPDSTPAIGPAKPDFFNR
                     IIANQKKGEAALDVYERIERVESRKNSGDPAPAAIKIMRVIPSGTGMFRIPVGADGKP
                     RDSAAYRADLEKLEKALALLVNDSRSQRDGLEKYAKKKKDRNDLIEATHNAFLFTLLA
                     NEPRGDRSLSRYKMEPNPAFKPTSRFTSIFPKVRGFVWVDEASGELARIEGEVTDDIS
                     VGLFLGKIYKGSHFMQERYEFQPGLWLTSFSQYDFDGRKLFSGFSVHERTFYSNYHYV
                     GPPKEALAAIRQELGHAELDKPRAGSADQ"
     gene            12933..13451
                     /locus_tag="VGR03_13960"
     CDS             12933..13451
                     /locus_tag="VGR03_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995431.1"
                     /translation="MPRSFSFAMIVTLFAAFSAAAQSPDSSVPALPAPSQDSAPPADA
                     KKPKKVWTNENLSGATGAVSVVGDPKNKPKPASSKPVNAQYVASVRKQLDKLQGQIAD
                     IDKQLVDLKNFSEGEPSTSASGIKLNKSYSREPIEVQMRALQDKKKDLESKMDALLDE
                     ARKNGVESSQLR"
     gene            complement(13488..14228)
                     /locus_tag="VGR03_13965"
     CDS             complement(13488..14228)
                     /locus_tag="VGR03_13965"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEV1995432.1"
                     /translation="MKRLLPVVVVGAALVGYIAGYASKPSPQQAPQAVLTGHFDDLSM
                     IPSANAQENKSEVMPFNFNSPREPQLAPDAPPVTYWNIDDIRKAHGELAERASKALKE
                     AGSGSSQSFGGGPVHISTRNFSIFMLYRAHREQPVLSLTKVNSVWDDAEQHAGAYDFY
                     VITGGTGEMIVGGKIANRQNLMDKDGPVPGEYRGQPVVGAQTYKVKPGDWLLIPPDTP
                     HQPKPDAGGFSYMIMKINVGVYPWSLIR"
     gene            complement(14301..14807)
                     /locus_tag="VGR03_13970"
     CDS             complement(14301..14807)
                     /locus_tag="VGR03_13970"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEV1995433.1"
                     /translation="MNALPCGCSIRQEGNDDAKMKYPAAILVLAAIASPLCMLRAQAP
                     EAESRSVWDGVYTEDQAKRGEPIYRKECAACHGDMLTGGESSPPLTGGAFLANWNGLT
                     MGDLFDRIRKTMPQSAPGRLTRQQDADILAFMLSVNKFPAGKTELYRRSEMLKEIRFE
                     SAKPTPKK"
     gene            complement(14804..>15055)
                     /locus_tag="VGR03_13975"
     CDS             complement(14804..>15055)
                     /locus_tag="VGR03_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995434.1"
                     /translation="VTKSLVVAGEGGFFTLPDGRRGAMLRAYDKATGKDAGEVYMPAP
                     QTGSPMTYMVKGKQYIVVAVSGGPYSGEYIAYTLPSTGE"
BASE COUNT         2955 a         4920 c         3854 g         3326 t
ORIGIN      
        1 acgttccccc gccgctcgga ccgcgcttcc gcatgcgccc ccgctccctc accgcccgcc
       61 caccgctgcg tccccgcccg cgctgacgaa acgacactcg caatctcatc tgaaccgacg
      121 taagggaact tttccgcgcg ctcggcctct atctctatag cgctttgcat tcgttaccgc
      181 agtcacaaca gcccagcgct aagaggtacc catgtccgaa tcctctcccc gccgttctct
      241 gccaaaacga atcgttctca tccttggagg gcttgcagta ttcacgcttg tgttcgtcat
      301 cgccattcgc ctcgcggtag tgggaatatt cagcggcatt gaacagaaca gagccacagg
      361 tctttccgca ttttcgcctt tcgctcacta ttcggccgcc tctgacttga tcacattggg
      421 gaacatttgg atctcgcgaa gtgctgctct acagatgtgc acggcctcct ttgaccggtc
      481 ggtggaggca ctccatcgca tcgtcgcctc tcgccatggt tatctggaag acttgcggac
      541 ggaaagccgg tcaggctctg gtcgcacact ttccgccaac gtttccgtgc cagcgagcga
      601 ctttgaggca gtactctccg aagtgaaaac actaggtcga atagaaacga tctcagaagc
      661 gggtgaggat tctgcggtca aaagcaactc cgcaacaaga cacctcgccg cggcacaaac
      721 caatctttct cgtcttcaga aattacagcg cgcacgaaaa ggtgaactgc gggacgcagt
      781 tgccttggaa aaagacattg cacaagccaa tgaagccgtc atcgaagcgg agcgccagag
      841 tcagggcttg gtctcaaccg tcgcacaagc tcatatccgt ctcgtgctgt tggaagatta
      901 ccgcgcccct ctacaagcca atcttgcggg tctcttccct cagcttcgaa attctctcgt
      961 cgagggagtt agttccatct tctcttctgc cgctctcgtt ctcggcgtcc tcttagaatt
     1021 cggcctgccg ctcttgtttt gggctgcgtt gctcttcgtc ccagcccgct tcgcctggcg
     1081 ccgctttcgt cgcgtccccg aagtgcttcc cgtatcccaa tgagtgagtt ccagtcgcgc
     1141 tttcaccctg aagaacaaaa aaaggccgga agcaatcgcg tccggcctct ctgcttgatt
     1201 atgtttgatc ctagccctcg acgaggcctt tctcgagcat ctcctcgtcg ctgagccaca
     1261 gatcggggat gttggctgag ccaagccgaa gaatgtcttc tagcgcgccg cgcacttctt
     1321 tcaagtggaa atccacgcca atgcgttcga gcaattcgat cagcgcttgc gcgccttccg
     1381 ccagcaccgg ctttacgggc gcaccgtcgt ggtccagccg ttgataggtc acgatgtaaa
     1441 tggccttggg tccgccgcca tgcctcacga tgtgcaggaa cccgtgagtc ttgggttttg
     1501 ccggggccag ccgtgctgct actcctgagc cggaggagag tgcgctcatc gttggtgtgc
     1561 tctcacctgt cccttgagtc tcagctgcat ccgctcgtgc tcccgcagtt ttcgcacttg
     1621 tagcacgcgc cgttcggcac catcagcatg ccgcactcgc tgcacgtcgg cgcgccctcg
     1681 tgcgcgttgc tcggtcttga cgccgacaac gccgtttgca tggcatccag gttggcctgc
     1741 gtcatcgccg gtagtaccgc tgccggggcg gccgtcgcag cgttctcagt tgccgccgct
     1801 ccattcaact tcaagtaatc cgcggagatg aatcgcccac ccagccaccg cgcgatgtag
     1861 tccagcaccg aagaaacaaa gcggatgttc gcgttcgcgg taatgccaga cggctcaaac
     1921 cgcgtgttca ccagcttgtc cacgaacact ttcagcggca cgccatactg caagcccagc
     1981 gaaatggtca gcgccaatcc atccatcacc ccggatagcg tcgagccttc cttggacatc
     2041 ttgatgaaga tttcgccggg ccgcccatcg tcgtacatgc ccacggtgat atagccttcg
     2101 tgcccgccca cgctgaactt gtgcgtcacc gaagcgcgtt ccacgggcat cttctcgcgc
     2161 tgccgccgcg caaacagctc gcgctgcatc ggttccacga cggctgcggt ttcagcctgc
     2221 ttttcttctt ttttcccgcc cgcgttgagc ggctgcgaac gcttcgaatt gtcgcggtaa
     2281 actgccacgg ccttcagccc caccttccac gactcaatat atgcgttcat aacgtcttcg
     2341 acggtcgatt cctcgggcat gttgatagtt ttcgaaatcg cgcccgagag gaacggctgc
     2401 gccgccgcca tcatcttcac gtgcccggtc caggtgatcg acctcccgcc gccctgcggc
     2461 gccagcgagc aatcgaacac cggcaagtct tccgctttca aatgcggcgc gccttcaatc
     2521 tttccgttct ggtcgatgta ggaaacgatt tcgctggtct gttccggagt gtagcccagc
     2581 ttcatcaatg cctgcggcac ggtgttgttc acgatcttga tcaacccgcc gcccaccagt
     2641 ttcttgtact tcaccagcgc cagatccggc tcgatccccg tggtgtcgca atccatcatg
     2701 aaaccgatgg tgccggtagg cgccagcacg gtcacctgcg aattcttgta gccatgcttt
     2761 tcgccatgcg ccagcgcggt gtcccacgat tcctgcgcgg ccatgaacaa ttccgtctgc
     2821 acgtgctccg gctggatccc gcgcatcgcg tcgcggtgca tgcgcatcac gtccagcatg
     2881 ggttcgcggt tcacttcgta gcccgggaac ggccccatgc gctccgcaat tctcgaggac
     2941 tgcgcgtacg cttcaccgca catcagcgct gtgatcgcgc cggacacatc gcgcccttcg
     3001 tcggagtcat acggcagcgc catggacatc agcagcgcgc cgaggttggc gtagcccagc
     3061 ccgagcggcc ggaagttatg tgaattctcc tcaatcttcg gcgtcgggta actcgcgttg
     3121 tccaccagga tttcctgcgc cgtgatcgtc acgtctaccg cgtgcttgaa cgccgccacg
     3181 tcgaactgcc cgctggcgtt cacgtacttc atcagattca gcgaagccag gttgcaagcc
     3241 gtgtcgtcca gaaacatata ttcgctgcac ggattcgagg cgttgattcg cgccgtgttt
     3301 ttcgaagtgt gccagcggtt gaccgtcgtg tcgtattgca tcccgggatc gccgcagtgc
     3361 caggtcgaat ccgaaatctt catcatcagg tcgcgcgccc gcagcttttc attcggctgc
     3421 ccgtcgagca cgttcttcgt ccaccaatcc ttgtcctcga tcaccgcgcg catgaagtcg
     3481 tccgtcacgc gcaccgagtg gttcgcgttc tggaagaaga tcgaagcgta cgcgtcgccg
     3541 tcgatcgagc tgtcgtagcc ctgctcgatc agcacatgcg ccttgcgctc ttccttcctc
     3601 ttgcattcga tgaactcgac gatatccggg tgatcgatat tcaggatcac catcttcgcc
     3661 gcgcgccgcg tttttccccc gctcttgatc acccccgcga aggcatcgaa gcctttcatg
     3721 aagctcaccg gaccagacgc aattccgccg ccgctcagcg tttccctgct tccgcgcagc
     3781 gtcgagaagt tcgttcccgt gcccgagccc cacttgaaaa gcatcccctc agtccgcgtc
     3841 agccccatga tcgattccat gttgtcgctg accgagttga taaagcaggc cgagcactgc
     3901 ggcttcggct gcacgcccac gttgaaccac accggcgaat tgaaggccat cttctgctcg
     3961 accagcaagt gagtcaattc atcgcggaag gcatcccgcg atggcatgtc ggcaaaatac
     4021 ccgcactctt cgccccaccg cacgatggta ttcaccaccc ggccgatcag ttggcgtacc
     4081 gatccttcgc gctgggcggt cccgggcttt ccgtggaaat acttgctggt gacgatgttc
     4141 gtcgccgttt gcgaccagct cttcggcact tccacgtctt cctgccggaa gatcgtcacg
     4201 cccttctcgt tcccaatcag cgccgtgcgc ttctcccatt caaccgcgtc gaagggggac
     4261 ttcttcccgt ccgtaaagta ccgctggaac ctcagacctt tccccgcgcc gctcgctgtt
     4321 ttctctgcag ctgcatccgt ggcaggggat ttcccgctca atgcatgttt caccgttgcc
     4381 atccgtccct ccgcgctctt gggtccccac tcgcgcgggg gcaccctctc attttactcc
     4441 tctgcgtccg tcccgcagag ctgccgcgct tcgcttcagg gtctgggctg aagttaaggc
     4501 agctttgatt cgatcggatc gtgaaagcgg gcagcgaaaa aaaagccgac cgtgttaggg
     4561 tcgcacttaa gttccagcgc aaaacgccaa tgccctgccg ctggccggtg cttcgcactc
     4621 agcctaacag caggtgctcc gcctcaagta cgggcgcgga gctggtactt cgcgctcgat
     4681 tttttcgctt agaacccggg ccgcctcgtg ggttcacacg gaccttgatc catccccgac
     4741 tcggtccgtc tggcgcttcc acgtcccccg aatctggatt cgaagtatca acggaagaaa
     4801 attttctgtc aagagaaaaa cgctaaatgt ggttgacgtt tccgtacttc gtctatttgt
     4861 agtggtgcga ttcctactca accactttcg cttcaacact tttcgcgtcc ttgcctctca
     4921 cgctacactg cttgcatctt attcgacatg aaacgctctc tcctaccact ggccatcctt
     4981 ctcgttcttc ctcttcacct tttcgcgcaa accgcagcgc cagccactgc ccttcccatc
     5041 gcgcagtcag cattcggaca aaaactgcac atctccggaa ttcctaatgc cgggagagtc
     5101 accgatgctc tgtatcgcgg cgcacaaccc agagaagtgg gcttctctga gctgaaaata
     5161 ttgggcatta ccaccatcgt tgatttgcgc agcgaggacc gtgaaaagat cgcatgggag
     5221 cgcaagcatg ccgaatcgct cggcatgcgc tttgtccaca tccccgtcga tggctggtct
     5281 gccccaacca acgaacaggt cgtccaattt ctctctcttc tccacgacaa tccgggacaa
     5341 aaaatcttcg tgcactgcca cttcggtgaa gatcgcacca gcgttttcat cgccgcttat
     5401 cgcatagcct ccgataaatg gcctgttgaa caagcaatta gagaaatgta tttcttcggg
     5461 ttcaacggct tatggcaccc cgccatgaaa tcatttgtcc acgatttccc ttcgcggttg
     5521 aattctgcgc ccgctctcgc tccattccgc gctctgcctt ctcaaccctc atccgccgga
     5581 tcgctcgttc ctccccggcc tagctgattt tttccttgac tttctgttaa ctcatggtat
     5641 acttacttct agctaataat ctgcctgggc ggtttctctt ttcacttctg ggtctctagt
     5701 tcgtcttcct tttaagtctt aactcttttc actacatgcg tttgcgcact cttttgcact
     5761 tctcaaatat acaacctttt tcttttcaag cggttacgca ctctttgcca aatacacccg
     5821 ggtgggggta tccgacccag ttcatctacg ccggagcgga gctcccaaat aagtcctttg
     5881 aaatcatatc gtttaccgac ccccaccccg taactccaat agaatcatat gctttcaaaa
     5941 ttataggggg gggacccccg tcgctttcca aaccactcgt ggcaactccg caaggaaatc
     6001 tcaaacagcc cacgccagca cctagatcat gaacattcga cgcgaaaact ctttgtcctg
     6061 actaaatact gaaaactaag aacttaggac cactcctctc ctagtaccac tgtaccctct
     6121 caaacggaac gcctgctcca ttccaccacc tagtcacaat tcgctatcct gtcgttgcga
     6181 agagaactat ctgcctgagg gtgataacgt gcaagtcgga cgctacgaga ttctcgaaat
     6241 catcggcacc ggcgcccatg gccgcgtggc acgcgcccac gattccatga tcggacgcct
     6301 cgtcgccatt aagctcttcc ccaaggaatt ggccagcggc gaagcgcgcc agcgtttcat
     6361 tcaggaagca cgcgtggtag gccagctgtc acatccttcg atcatcaccc tccacgatat
     6421 gggcatcgac gaagccacgc aaacgcccta tctggtcatg gaatttctgg aaggtcagcc
     6481 gctcgatcgc attttggaga agggcagcct tcctttccca aaggcctgcg cttgggccgc
     6541 ggaagtggct tgcgcgctcg gtgtggcgca ccgtaagggt gtgattcatg gtgacgtcaa
     6601 gcccgcgaat atgctcatca ccgatgacgg tcgcgtgaag ctgatggact tcggcatggc
     6661 gcggctggcc agccgcgatt ccggcgccac tccgctactg ggcacacccg cttactggtg
     6721 tcccgagcaa atcatgggta aaccccagga cgcgcgctcc gatctttttt ccttgggtgt
     6781 cgttctctac gaaatggtga cgggcaagcg tccgttcgac gccgattcac ttccggccat
     6841 ttgcggacgt gtgctctctt cgactcctct accgccgtcg cacgccaatc cttccgtgcc
     6901 agcgggattt gatgcggtcg tcgcgcgctg cctcgcgaaa gatccggcgg cccgctatgc
     6961 aacggccgag gctctcgccg aggaactcta tccgctcgcg cgccacaaga ccgctcccgt
     7021 tcccgtgctt cattccaatg gcaatggcct gcgcgatcgc gctgcgcgtc tcctgcgctc
     7081 tgcctgatga tcacctcgca aaaaggccgc tacgaagctc tggagttgat cggctccggc
     7141 acgactagcc gtgtcgaaaa agcacgcgac aatgtcattg gccgcaccgt ggccctcaag
     7201 acctttatca acaacttcga cgaacacctc gaacagcagt tcctgcgtga agcgcagctt
     7261 gtcggtcagc tctctcatcc cgctgttgtg cagctttatg acgtgggaat cgaccagcac
     7321 ggcactccat tcctggtcat ggaatatgtc gccggcaaaa ctctcgaaca gcacctcgag
     7381 gcctccactc tgacgattca gcgcgcctgc gcctgggccg cggacctagc taacgcgctc
     7441 gcattggccc atcgcgcagg aatcattcac ggcgacgtca agcccggaaa cattctggtg
     7501 acgccggaaa acaaggtaaa actcggcgac ttcggcatcg cgcgcctgac cacacagctc
     7561 tccgcttccg gccagttgat gggcacgccc gcctatctgg ctcccgaaca gattcagaac
     7621 gaatttcagg atcaacgctc ggatcagttt tccttcggcg tagtttttta tcagatgctt
     7681 accggcattc gcccgtttgc gggaaactcg ctcggcgcag tctgcgcgca gattctgaac
     7741 gatgatcccg tgccgccctc gcgtcacaat ctggccgtgc cgcctgcgct cgaccgcgtc
     7801 gtcgcgcgtt gcctggcaaa aaatccacag gaccgtttca cttcctgcga agaccttgcc
     7861 aagtctctct atcccttcac ccgctcgcgc ccgcgaactc ccgagccggt cttcaagaag
     7921 cgctcctggt ggtctgcgcc ggcaggacat cgcgaagtat ggatggccgc atccgcctgt
     7981 ctgctcttgg ctgcgtttgt tcagattccc cgtttgctca gcgcccgcta tggaattcct
     8041 ccggctccca cgcgccagta ttttcatccc ggcgtgccgt acgaagcgtt tgtctacacg
     8101 cggcaaaccg ttgcgcaggc agccgcgcct gacgccccag gtgacttcga tccacgcatg
     8161 gcagacaaac attcttcccc gcgcatcatt cgtgtggaga aattagctct cgcgcaacta
     8221 aataagcccg ccatttcgca gaacctttcg tcggattcct catcggcctc ggcacgatcg
     8281 ccacagtcga acacgcctta gcccaggaat agaaaactcc tccaaggaaa tatcgcttgc
     8341 tggagagttg gttggaaatt agaagagaag aaattcgatc gcgctactcg taccgcagcg
     8401 cttccaccgg atccagcttc gccgctttca tcgccggcca cacgccgaag atcagtccca
     8461 cgcagacgct aaccacaatt cctgtgaccg ccgcccacaa cggcacgctt cccggccatt
     8521 tcagtccgac gtgtacgaga aacaccagcc cattcaccag caccagcgcg attacccctc
     8581 ccgcgcctgt cagggccatc gcctccagta ggaattgcca ggtgatatct ccgcttttcg
     8641 cgccaatcgc cttgcgaatc ccgatctccc gcgtgcgctc cgtcacgctc accagcatga
     8701 tgttcatcac gcccactccg ccaatcagca gcccgatccc gctcagcacc acggtcgcca
     8761 gagcaaccat gccgagaatg ctatgaaatt gttctacttc ctgctgtgac gtctggatcg
     8821 aaaaattatc cttgccgtca tagggaacat tgcgccgccg ccgcagcact tccgtggttt
     8881 gatccaccgc ctggtccagc aggtttggaa acgcctgcaa gcggatctgg ttctcgtacg
     8941 cgccgggata aatcttgcga aacgtgttgt agggaatcaa cacgcgccgg tcttcatcgt
     9001 cgtttccgaa catgcccttg ggcttttcta ccacgccgat cacctgaaac gccgaaccgt
     9061 cgatcatcac gtctttgccg accggcgaac cctcgggaaa aagcaccggg gccgcgtttt
     9121 cgcctagcag gacgaccttc tcgcgatgca agtcatcgcc ttcgctgatg aatcgtccct
     9181 ccagcgttgc ggcgttggca tagacttgtc cgaagttcgg ttgcacgccg cgaaaatcga
     9241 tggcgctcac acttcctgat ttcgtccgca cggtgtgctg ctgctcccac caggtcagga
     9301 atacggtcac gttttttact gccgggcagc gttcttgaat tgcctgcgca tcctcaagcg
     9361 tcagcggctt gcgctcgcgc tcgtccttgg gccggcggcc gttgtgtggc ccatggtcaa
     9421 agcgcgagat gaacgccgtg tccgcgccgt attgcttgat attttcctgg acctgggagt
     9481 cgaacccagt gatcagcgcg gccaccagca tgatgacact cacgccgagt accacgccga
     9541 agatcgtgag gaagctgcgc aatttgtgcg ctcgcagcgt gtccattgcc tgtttcagat
     9601 tttcgccgtg tgcttgcgcc atgccgtcct cagccgtccg cccgcagcgc ctcgatcgga
     9661 tcgagtttcg ccgcacgcat cgccggataa attccaaaga aaagccccac cgccgtggag
     9721 acacagagcg agagaatcac cgcgctcatt ggcgtgcgca tcggaatcga cgtggcggcg
     9781 cgcgccgccg caaccatcgc atacgccaga gcaattccca tcaccccgcc cgtcgccgcc
     9841 agcactgcgg actccgtgac gaactggaga acgatgtgct ttctccgcgc tcctagcgag
     9901 cgccgcagac ctatctcccg ggtgcgttct gtcacgctgg ccagcatgat gttcatgatc
     9961 acgattccgc caaccaccag gaacaccgcc gtcaaaccca cggccacggc gaacaggttt
    10021 ccggtcagtt gtttccacaa acccattatt gattccgagc cgacgacgcc gaaactgtcg
    10081 tccgcttccc aggggatatg ccgccaggca cgcaataaca tgcgcgcctc gtcttccgac
    10141 gccggtatca actccgcatt ccgcgcctgc gcaaaaagca agagcgaatc tctctgcgtg
    10201 ccccattcct tgatgtaagt gcccagagga ataaacacga aattgtcctg cgattggccg
    10261 aacgcactcc caatgaccgc tgccacgccg atcacttcat aactgtgcgt cccggcgcgg
    10321 acggttttgc caatcggatc cacgttgcca aagaatttct tcgccaggtc cgcgccgatg
    10381 aagcacacct ccgagcggtg cgtttcgtcc gcttccatca ggaacctgcc cgccgcgaca
    10441 ctcaaattcc gaacctcgat atagttcgcc gtcacaccga gaatattcac atcctcctgc
    10501 agcgtgtttc cggagcgcac atccgaggag gtgccgagga gcgccgctat ttggctcgta
    10561 gtgcgcatcc cttctttgag gctgccaaat tcttcacggg tgatgagcgg ccgcttctgc
    10621 gcttttatcc aggcgtcttc gctggtaatg attccgaatc gcgaaatgac atacacgttt
    10681 gcgccaagat tcgcaacctt gtccgccaca taaaaattca gccccgcaac cacgctcatc
    10741 accaccacca gcgtggttac tgccaggatg actcccagca gtgtcaggaa gctccgcagc
    10801 ttgtgcgcgc gcaacgagtc cagcgccagg cgcagcgtct cgcccatggt gaccagcgcg
    10861 ctccgcagcc ggccgagctt cccggcaata tccagccctt gcggcaccac gcgtgggatt
    10921 gcgcctacct ggccaatggg ggttacaccc aaacagacct ccaaccttct gtatctaaga
    10981 cgaatgcgcc cgtcaattcg ttccttgcta tatagacgtg tggaactgcg ctttgctatc
    11041 agctcatctt ggagtagcct tacacccggc ccggtccaat ctcaatggtt tagccgtccc
    11101 aaactcaatt gtaccgcctt gtacgacaaa tccaggaaga agatcgcgga gcaggcctgt
    11161 gagactgact ctgcatcaag aggaggacgc tatgcgtcgc atttgccttt attttcttgt
    11221 acttacagta ttcggtctga accttatgcc gtccagccaa gcccaggata ccctcgacca
    11281 taaaacgccc gccatggctt ccgccgcacc aacgtcaggc ttccgcgccg aattcctgga
    11341 agagatcacc tactatgagc agcgatacac tcgcctggcc gaggccatgc cggcggaaaa
    11401 gtacgtttgg cgtcccgccg aggacgttcg ctccgtaggc gaagttttca cccacatcac
    11461 tgctgcgaac tacggcattg ctcgggccct cggcactgcg cctccggcga gcttcgaccc
    11521 gaaggccatc acggctctct cggctgataa gccaaaggtc ttgcaggcct tgaaagattc
    11581 cttcgcgcat ttccgcggtg ccatcatcgc ttttaacgag gccgatggcg acaagcctca
    11641 gaacatattc aaccgtcaca cgaccctgcg cggttctttc atcgccatca ccggacactt
    11701 tggcgagcat ctcggtcaat ccatcgccta cgcgcgcgtg aacggcgtcg tgccaccatg
    11761 gaccgaagac ttccagaagc aacagcaaaa acccgccgac aagcctaagc cataaccatc
    11821 tcggaggagc aagctgccat tgacgctttg acacccgccg gagcacttcg gcgcattggc
    11881 ggtgtacaac aattgtgtgc ccggacgaat ccaatcagac tagacgttgg cgggtttcta
    11941 gctacttcgc atgatgctca aacgtacatt tattaccatc gcgctgctgt ctttggttct
    12001 cgctgctgac gccgccgcgc aatccgtcgt accggattcc acgcccgcga ttgggccggc
    12061 gaaaccggat ttcttcaatc gcatcatcgc caaccaaaag aaaggcgagg ccgcactaga
    12121 tgtctacgaa cgcatcgaac gcgtcgagtc ccgcaagaat tctggcgatc ccgcgcccgc
    12181 cgcgatcaaa atcatgcgcg tcattccttc ggggaccggc atgttccgga ttcccgtcgg
    12241 cgccgatggc aagcccaggg attccgccgc ttatcgcgcc gatctcgaga aattggagaa
    12301 agccctcgcg ctgctcgtta atgacagccg ctctcagcgc gacggtctgg aaaaatatgc
    12361 gaagaaaaag aaagatcgca acgacctcat cgaggccacg cacaatgctt tcctatttac
    12421 tcttctcgcc aacgagcccc gcggcgaccg ctcgctttcc agatacaaaa tggagcccaa
    12481 cccggccttc aaacccactt cacgcttcac ttccatcttc cccaaagtgc gcggctttgt
    12541 gtgggtcgac gaagcctccg gcgaacttgc ccgtatcgaa ggcgaagtta ccgacgacat
    12601 ttccgtcggc ttgtttctgg gaaaaatcta caaaggcagc cactttatgc aggagcgcta
    12661 cgaatttcag cccgggctgt ggctcacgag cttttcgcag tacgatttcg acggacgcaa
    12721 gttgttttcc ggtttctccg ttcacgaacg taccttttat tcgaactacc actacgtcgg
    12781 tccccccaag gaagccctcg ccgccattcg ccaggaactc gggcatgccg agttggacaa
    12841 gccgcgcgcc ggctccgccg atcagtaaaa cttttccttc tagaatgggt ttaagacggc
    12901 agaatcctcg accttgagag actggaggct ttgtgccccg ttcgttctca ttcgcaatga
    12961 tcgtgactct tttcgcggcc ttctccgctg ccgcgcaatc tccggacagc agtgtgcctg
    13021 cgctgccagc cccatcgcag gattccgcac cgcctgccga cgctaaaaaa ccgaagaaag
    13081 tctggaccaa cgaaaatctt tccggcgcga ctggcgctgt ctccgttgtc ggcgacccaa
    13141 agaacaagcc gaagccggca tcttccaagc ccgtaaacgc gcaatatgtc gcatcggtcc
    13201 gcaagcagct cgacaagctg caggggcaga tcgctgacat cgacaagcag ctcgttgatc
    13261 tgaagaactt tagcgaaggt gaaccatcga cgtccgccag cggcatcaaa ctcaacaaga
    13321 gctacagccg cgaacccatc gaggtgcaga tgcgcgcgtt gcaggacaag aagaaagatc
    13381 tcgaatcaaa aatggacgcg ctcctcgacg aagcccgcaa gaacggcgtc gagtccagcc
    13441 aactgcgctg agttcggcgc gccgcattct cttctttctg cgcttgacta gcgaatcagg
    13501 ctccaggggt acacgcccac gttgattttc atgatcatgt agctgaaccc gccggcgtcg
    13561 ggcttgggct ggtgcggcgt atccggcgga atgagcagcc aatcgcccgg tttcactttg
    13621 taggtctggg caccgacgac aggttgtccg cggtattcgc caggcacggg gccgtcctta
    13681 tccatcagat tctggcgatt ggctatcttt ccgccgacga tcatctcacc ggtgcctccg
    13741 gtgatgacgt agaaatcgta agcaccggca tgttgctcgg cgtcatccca aacactattg
    13801 accttggtca agctcaggac cggctgttcg cgatgcgcgc ggtagagcat gaagatgctg
    13861 aaattgcgcg tcgaaatatg caccggtccg ccgccgaatg actgcgagct tccgctgccg
    13921 gcctccttta acgccttgga agccctttcc gccagctcgc cgtgcgcctt tcgaatgtcg
    13981 tcgatattcc agtaggtgac cggcggggca tcgggcgcca actgcggctc gcgcggcgaa
    14041 ttaaaattga acggcatgac ctcggacttg ttttcctggg cgttggcgct cgggatcatc
    14101 gagagatcgt cgaagtgccc ggtgagaacg gcttgcggag cctgctgcgg actcggcttt
    14161 gaagcgtacc cggcaatgta accaacgagt gcggcgccaa ccaccacgac aggaagtagt
    14221 cttttcataa agcaatcctc cgtttcaaaa atatcgaaga actcttttct tacgatggcg
    14281 aaaaccactc catgcccggt ttatttcttg ggagtgggct tcgccgattc aaatcgaatc
    14341 tctttgagca tctccgaccg ccggtagagt tccgttttcc cggctgggaa tttgttgacg
    14401 ctcagcatga aggccagaat gtctgcgtct tgttgccggg tgaggcggcc cggagcgctc
    14461 tggggcatgg tcttgcgaat tctgtcgaac aagtcgccca tcgtgagtcc attccaattc
    14521 gcaaggaacg cgccgcctgt cagcgggggc gaggattcgc caccggtgag catgtcgccg
    14581 tggcatgccg cgcattcctt acggtaaatc ggctcgccgc gcttggcctg gtcctcggtg
    14641 tacacgccgt cccacacgga acgcgactct gcctctggcg cttgcgcccg aagcatgcaa
    14701 agcggcgaag cgatcgcggc cagaacgagg attgccgccg gatatttcat cttcgcgtcg
    14761 tcattgcctt cctgtcggat ggaacatccg catggcagcg cgttcattcc cccgtcgatg
    14821 gcagcgtgta agcaatgtac tcgccggagt aaggccctcc gctgacagcc acgacgatgt
    14881 actgcttgcc cttcaccatg taggtcatcg gcgagccggt ctgcggcgcg ggcatgtaca
    14941 cctcgccggc gtctttgccc gtggccttgt cgtatgcgcg cagcatggcg ccgcgccggc
    15001 cgtcgggcag cgtgaagaat ccgccttcgc cggcgacgac gagcgacttg gtgac
//
LOCUS       DASYVO010000417         1502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_6951, whole genome shotgun sequence.
ACCESSION   DASYVO010000417 DASYVO010000000
VERSION     DASYVO010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6951"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..541
                     /locus_tag="VGR03_13980"
     CDS             59..541
                     /locus_tag="VGR03_13980"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1995435.1"
                     /translation="MLIRAAAAGDANAIWAIMEPVVRAGETYTLPGDMDKKSALKYWL
                     SAEHEVFVAEDNNEIVGTYILRANQKGGGAHVANCGYMTAVSAPGRGIARVMCSHSLD
                     HARARGFRAMQYNFVVSTNERAVRLWQSFGFEIVGRLPRAFHHPALGYVDAYIMYRDL
                     "
     gene            584..1183
                     /gene="recR"
                     /locus_tag="VGR03_13985"
     CDS             584..1183
                     /gene="recR"
                     /locus_tag="VGR03_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEV1995436.1"
                     /translation="MPDFAAPVERLIDELKHLPGIGQKTAQRLAFYLLRAAPEDALAL
                     ADAIRDAKDKIRACSVCHNITDSDPCLYCVGPTRSKKTICVVEEAHNIMAIEKTRTYS
                     GMYHVLGGSLSPLSGRGPEQLHLKSLIERLKGGGVEEIIIATNPTAEGEATAVYISKL
                     LKPLGVRVTRIGVGIPVGADLEYADEVTMMKAMEGRRDL"
BASE COUNT          327 a          408 c          465 g          302 t
ORIGIN      
        1 gggccggcct tcgggctggc ccgtctgcgt ttaagaacaa acgtggcaag atacgaagat
       61 gctgatccgg gctgccgcgg cgggtgatgc gaatgcgatc tgggcgatca tggagcccgt
      121 tgtccgggca ggagaaacct atacgctgcc aggcgacatg gacaaaaaga gcgcgctcaa
      181 gtattggctt tcggctgaac atgaggtctt tgtcgcggaa gacaacaacg agatcgttgg
      241 cacctacatt ctgcgggcca accaaaaggg cggcggagcg cacgtcgcca attgcggtta
      301 catgacggcc gtctcggcac cgggccgggg tatcgcacgc gtgatgtgct cgcattcgct
      361 cgatcacgct cgcgcgcgtg gattccgcgc gatgcaatac aatttcgtag tcagcaccaa
      421 cgaacgagct gtccggctct ggcaaagttt cggatttgag atcgtcgggc gccttccccg
      481 cgctttccat catcccgcgc tgggctatgt cgacgcctac atcatgtatc gcgatctcta
      541 gggcagtttg cgctgatgcg cgtggctatg ctacacatgc tgcatgccag attttgccgc
      601 tcctgtcgaa cgactcatcg atgaattgaa acatctccct ggaattggcc agaagaccgc
      661 gcagcggctg gcgttttacc tgctgcgcgc ggcaccggaa gatgcactgg cgctggcgga
      721 cgcgatccgc gacgcaaaag ataagattcg tgcttgctcc gtttgccaca acatcacgga
      781 ttcggatccg tgcttgtact gtgtgggccc gacgcggagc aagaagacga tttgcgtggt
      841 tgaagaagcg cacaacatca tggcgatcga aaagacgcgg acctacagcg ggatgtatca
      901 cgtgctgggc gggtcgctct cgccactttc cggacgcggc ccggaacaac ttcacctgaa
      961 gtcgctgatc gaacggctga agggcggcgg cgtcgaagag atcatcatcg cgacgaatcc
     1021 cacggcggaa ggcgaggcga cggcagttta tatttcgaag ttgctgaagc cgctgggcgt
     1081 gcgcgtaacg cggatcggcg tagggattcc cgtgggtgcg gacctcgaat acgcagatga
     1141 agtaacgatg atgaaggcga tggaaggacg aagagacctg tagacacttc acacggcaga
     1201 tggggcaaga gagaaagagg ttccagtcta aagccaggtt cttcaagagt tttcacgagg
     1261 ccaaattcca gccaaattct agtactccta gaacgctcta gccaattgcc ccatttcggt
     1321 atagagtttg aattccgcgg tggttgtcct cccgccgatt gaatggcagc cgcggcgcgc
     1381 ggctgacagg aggtgcgctt atgccgagct tggcgcacta catggcgcag tacgaccacg
     1441 aacacgagtc tgcctggaac aaattcctgc acggtgttgg catcccgatg atttttgccg
     1501 gg
//
LOCUS       DASYVO010000418         9960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_168053, whole genome shotgun sequence.
ACCESSION   DASYVO010000418 DASYVO010000000
VERSION     DASYVO010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9960
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168053"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2089
                     /locus_tag="VGR03_13990"
     CDS             <1..2089
                     /locus_tag="VGR03_13990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1995437.1"
                     /translation="QPEQLSGKKVTPNFFDVLGVQPVVGRPFTAEEDKSQSPVVVLSY
                     NLWRRRFGGDKSIIGRSILMSGVQTKVVGVMPKDFFFGDRKNSDYWVPMAFTPEQWAQ
                     RQSHFLTVVARMKPGVTTQQAQKDMEQIAANLQKQYPESNAKLGAVVVPIQKNYAGDA
                     RSGLLVLQVASLFVLLIACSNLANLLLARSTSRRREMAVRLAMGASRWQIASQLLTES
                     VVLSVAGGLLGLWVGQTCWSVFGQLVPEQAGEGFALNGHLLLFTAGISIAAGMLFGFA
                     PALWASSVPLQESLKEGGRGGESRGGLRLRDALVVGQFALAFALLVCAGLMIQTIWNL
                     RKQELGFRADHLLTMGVPLPEKKYDTDEKVRGFFRAVVEDVRALPGVKGAGFGSDAPF
                     MTIGDTEGYTVEGEPPLPKGEYNDALYREVTPGYLEMLGAKVKEGRFLEESDHEGGLP
                     VLVVNEFLAKRHWPGKSAVGKRIRFGGEKAPWWTVAGVVRDIRERGLLYEMKPAVYVP
                     VSQVKKPSGFAFLVVRTSNDPLSTIKMVEGAVWSVDPQQPVSYVRTMDQLIETDVADR
                     TRPMILLGVFAGLALVLACLGVYGVLAYAVAQRTREIGVRMALGAKPMDVTRMILGRG
                     MKLSAIGLLAGGGLAAGLAVLLRTPLFGVTLVAPGIYAGTAAALVLVALVACVIPAQR
                     ASRVDPAVALRNE"
     gene            2288..2677
                     /locus_tag="VGR03_13995"
     CDS             2288..2677
                     /locus_tag="VGR03_13995"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV1995438.1"
                     /translation="MRRAFVFITIFLLTLVAVRGVSAAPQGRASSTLSGVVLGPDDKP
                     VANASVSYQLSSGSAPHAVHTDAHGHFSISKLKADNYDLRASGKGVFSEWQKNISLRS
                     GTTKELTVRLIYAKQIPKAYVKSNPKK"
     gene            complement(2794..3549)
                     /locus_tag="VGR03_14000"
     CDS             complement(2794..3549)
                     /locus_tag="VGR03_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEV1995439.1"
                     /translation="MSCDFTRTVLHGYLDGELDAPRAAEFERHLESCRECATSLGAEE
                     SLRSSLQRSGLYENAPVALHKKIRAGLDAAMPSSGFIRIPAWRWLAVAAAILIAASVS
                     WFAWPRVQNSAASSPFTAAEIIDAHVRSLQPGHLTDVASTDQHTVKPWFDGKLAFVPP
                     VKDFADEGFSLVGGRLDVLGGQNVAALVYSRRKHIINVFVLPTKEPDTPIHPPGLRQG
                     YQWRHWRRQGMEYCAISDVSDSDLYELAQLLYQ"
     gene            complement(3539..4105)
                     /locus_tag="VGR03_14005"
     CDS             complement(3539..4105)
                     /locus_tag="VGR03_14005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1995440.1"
                     /translation="MSGFDQGKGSDVLDARDRARFEQLVLPHLDAAFNLARWLLRSRA
                     DSEDVAQDAMLRAYRFFRGFHGGDARAWLLQIVRNTCYTWLEKNRPVELMTEFDEELH
                     PQPSTSPEALAIAGDNRERLTRAIDTLPPRFREVLVLRELEGCSYKEIAAITAIPIGT
                     VMSALARARQRLQRTLTHASPQEASREL"
     gene            4993..5877
                     /locus_tag="VGR03_14010"
     CDS             4993..5877
                     /locus_tag="VGR03_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995441.1"
                     /translation="MRQVKAMNSTAARVIACLLVALALHQISACVARAGEPALPKLKT
                     ETRGAFERYLELAEARNEGELKRGTALLWVDGLPEEQRAEAYAALKRGEVKMQKLEIL
                     DNAKTIPCPGGMIHHWTGVVFIPGAKLEDVLGVLEDYDRHSVYYAPDVERSKIESREG
                     DHFRVFLRFRRQKVITVVLNTEHEVQYFRDAPGKAHSRSLAVRIAEVENAGKSDEREK
                     TPGDDDGFMWRMETWWRMEERDGGVYVQSEVASLTRDIPTGLGWMIKPFVTDIPKESL
                     TFTLQATRRAVEARRASR"
     gene            complement(5892..7112)
                     /locus_tag="VGR03_14015"
     CDS             complement(5892..7112)
                     /locus_tag="VGR03_14015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1995442.1"
                     /translation="MRLAAVSPFVDRRHGTERALAELLERLARKEHCEIHLYAQRVED
                     LALSQPGVTHAQNSGAIIWHKVSSIPGPHLLQFLAWLFLNSICRAWDRWVHGLRFDLV
                     VSPGINCLDADVVIVHALFHRLQELAHEEKLDSARPSLLRRLHRRAYYALLAGMERRI
                     YADPRVSLAAVSQRTATLLADYFRRQDVRVIPNGVNTAQFSPSARLALRAEARRRRAI
                     QETDFVLLLIGNDWRVKGLETVLRAMSILRELPTLVIAAGDDSPDSFRETAKSLGISE
                     RCRFEPSRENVLDFYAAADLYVSPSREDSFGLPVAEAMACGLPVITSMHAGMADLIRD
                     GMDGFILRQFDDFHGLAGTIQQLHADEVLRKNVGDAAAQAARAWTWDRNAADVWELLK
                     DAANKKSPPPARKP"
     gene            complement(7114..8706)
                     /locus_tag="VGR03_14020"
     CDS             complement(7114..8706)
                     /locus_tag="VGR03_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995443.1"
                     /translation="MRLDSQDSPYQFRRSTLFVFGFLVFFVVVAYKTAGYVIVGDMTG
                     LAFAAMAFVGCAFIVAMLNNWRNGVYFFLTWLLFEDFARKYLGNNMAIYFAKDVLAAV
                     VYLSFFLAYRRKEVTSFRPPFLVPVLIFVWFGVMQVFNPASTNLMYGVLGLKLFFYYV
                     PLLFVGYALLNSEAELRRFFFVNMILALIIISLGIAQAIIGHTFLNPEIIAADIRDLS
                     TLYRVSPISGLIMYRPTSVFVSTGRFGNFLLVTWLLVFGFSGYLLLRHKRGRALAFIV
                     LALTVAGLALCSSRGVVLWSSGSALVGSAAFVWGAPWRQREVMRVLRSVQRTALGIAL
                     GIVVLLATYPEALLSRVAFYTETLSPSSSASELQHRTWDYPLTNFLGAFGYERWIYGY
                     GIGTTSLGTQYVARIFGAKPPVTGVESGFGTLVIEMGIGGLVLWFVMSFAILFAAWRV
                     VKKLRGSPWFPLAFVIFWYAGLLFLPMTFSGIQAYEDFVLNAYLWLLLGVLFRLPTLA
                     VSAQFAATAQLSPMYDPRIRMR"
     gene            complement(8775..>9960)
                     /locus_tag="VGR03_14025"
     CDS             complement(8775..>9960)
                     /locus_tag="VGR03_14025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1995444.1"
                     /translation="SHFFAPSIGGVESAVLSLAGGLRKLRTPQGDSEFDVTLVTHTPA
                     GDFDDRSLPFRVVRQPSVLQLWRIIRKSDVIHIAGPALPPLVLGSLARKPVVVEHHGY
                     QAICPNGLMVHQPDGSICPGHFQAANYAKCLRCRASETSGLRSLAELLLMFPRFWLCR
                     RVSTNLAISHHVLERHGLPRSSVVYYGIEDPLGKDTVPSSAANATGKVCFAYVGRFFP
                     EKGIAILLRAAGMLREEGCEFEVRLIGDGPQRPKLEEIIEANCLGSCVRITGYLTGAA
                     LADALRDVRVVVMPSVCEETAGLAAIEQMMRGRLVIASRIGGLSEVVGDCGLQFPAGN
                     AEALAECMKRVMQDASTIDSMGLKARERARQLFQRVRMVEEHAIAYREIPRGGSKRLE
                     QN"
BASE COUNT         2337 a         2608 c         3185 g         1830 t
ORIGIN      
        1 gcagccggaa cagctctccg gaaagaaggt gacgccgaac ttctttgatg tgctgggagt
       61 gcagccggtg gtgggacgtc cgtttacggc ggaagaggac aagtcgcaaa gtccagtggt
      121 ggtgttgagt tacaacctgt ggcgcaggcg cttcggcggg gataaatcga tcatcgggcg
      181 gtcaatcctg atgagcggag tgcagacgaa agtggtcggc gtgatgccaa aggatttctt
      241 tttcggcgac cggaaaaaca gcgactattg ggtgccgatg gcgttcacgc cagagcaatg
      301 ggcgcaaagg caatcgcact tcctgacggt ggtcgcgcgg atgaagccgg gcgtaacgac
      361 gcagcaagcg cagaaggaca tggaacagat tgcagcgaat ttgcaaaagc agtacccgga
      421 atcaaacgcg aaactgggcg cggttgtcgt gccgattcaa aaaaactatg cgggagacgc
      481 gcggagcggg ctgctggtgc tgcaagtggc ttccctgttc gtgctgctga ttgcctgttc
      541 aaacctggcg aacttattgc tggcgcgatc gaccagccgg agaagagaga tggcggttcg
      601 gctcgcgatg ggtgcatcac ggtggcaaat tgcttcgcag ttgctgaccg agagcgtggt
      661 gctgtccgtt gcgggcggat tgctggggtt gtgggtcggg cagacgtgct ggagcgtgtt
      721 tgggcagctg gtgccggagc aggcgggtga gggattcgcg ctgaacggcc acttgctgct
      781 attcacagca gggatctcga ttgcggcggg aatgttattc ggattcgcac cggcgctttg
      841 ggcgtcgagt gtgccgctgc aagagtcgtt gaaggaggga ggccgcgggg gagaatcgcg
      901 tggcggattg cggctgcggg atgcgctggt ggtgggacaa tttgcactgg cgttcgcgtt
      961 gctggtatgc gccgggctga tgatccagac gatctggaat ctgcggaaac aagaactggg
     1021 atttcgggcc gatcatctgc tgaccatggg agtgcctttg cccgagaaga agtacgacac
     1081 agacgaaaag gtgcgcggat ttttccgcgc cgtagtggaa gacgtacgcg cgctaccggg
     1141 agtgaaggga gcgggatttg ggtccgacgc gcccttcatg accatcggag ataccgaagg
     1201 ctacacggtg gagggcgagc cgcctctgcc gaagggtgaa tataacgacg ccctctaccg
     1261 ggaagtaacg ccgggctatc tggagatgct gggagcgaaa gtgaaagaag gccggttttt
     1321 ggaagagagt gaccacgaag gcgggctgcc agtactggtg gtgaacgagt ttctcgccaa
     1381 gcggcactgg ccgggaaaga gcgcggtcgg gaaacggatt cgcttcgggg gcgagaaagc
     1441 cccctggtgg acggtggcgg gagtggtgcg cgacattcgg gagcgcggac tcttgtacga
     1501 gatgaagccg gcagtgtacg tgccggtgtc gcaagtgaag aaaccgagcg ggttcgcgtt
     1561 tttagtcgtg cggacgtcga acgatcctct gagcacgatt aaaatggttg aaggagcggt
     1621 gtggtcggtg gatccgcagc agccggtatc ttacgtgcgt acgatggatc aactgattga
     1681 gacggacgtt gccgaccgga cgcgcccgat gatcctcctg ggagtgttcg cggggcttgc
     1741 gctggtgctc gcgtgcctcg gcgtttatgg cgtgctggct tatgcggtgg cgcagcgcac
     1801 gcgggagatt ggagtacgca tggccctggg ggcaaagcca atggacgtaa cgcgaatgat
     1861 tctgggtcgc gggatgaaat tgagtgcgat cggattgttg gcgggcggag gactggcggc
     1921 ggggctcgcg gtcttgttgc ggacaccgtt gttcggggta acgctggtgg cgccaggtat
     1981 ttatgcgggg acagccgcgg cgctggtcct ggttgccctg gtggcgtgcg tgattccggc
     2041 gcagcgcgct tcgcgcgttg atcctgcggt ggctttgcgc aacgaataag agcacaaatg
     2101 aattcgggat caccagcctg tccgaattgg caggttggcg gtgaggcaca ttctcctcct
     2161 gcgacggttc gtcctgccag acacgtttta cactcaaggt tgaaagggac ggaacagcct
     2221 caagctcgaa ttgaggcgca atccttctac agcgtttccc gcgggggaca ctggcaaggt
     2281 tgaaaaaatg agacgcgcat tcgtattcat aacgattttt ctgcttacgc tcgtggcggt
     2341 tcgcggcgta tctgccgcgc cgcaaggccg ggcatcctcg acgctgagcg gagtagtgct
     2401 ggggccggat gacaagccgg tggcaaatgc gagtgtgtcc taccaattgt cgagcggcag
     2461 cgcaccacac gcggtacaca cggacgcgca tgggcatttc tcgatttcga agttgaaagc
     2521 ggacaactac gatcttcggg cttcgggaaa aggtgttttt tctgagtggc agaagaacat
     2581 ctccctgagg tccgggacga cgaaagaact gacagtgcgt ttgatctatg caaaacaaat
     2641 tccgaaggcc tacgtgaaga gcaaccccaa gaagtgactg gtgattggtg actggtgcaa
     2701 attcgttgtt gcgcctgggt aagaatctcg ggcgatggcc gcgcagaact attcccgcgg
     2761 taaaacgaat ttcttattgg tagatgcgct gggttattgg tagaggagct gggcaagctc
     2821 gtacagatcg gaatcagaga cgtccgagat ggcgcaatat tccatcccct gccggcgcca
     2881 gtggcgccat tgataccctt gccggaggcc gggcggatga atcggcgtgt ccggctcctt
     2941 ggtgggcaga acaaaaacgt taatgatgtg cttgcggcgt gaatagacca gagcggcgac
     3001 attctgtccg ccgagaacat caagacgccc accaaccagc gaaaaacctt catccgcaaa
     3061 atccttcacc gggggaacaa aggcgagctt gccgtcgaac caaggcttga cggtgtgctg
     3121 gtcggtggaa gctacgtcgg taaggtgacc gggctgaagc gagcgcacgt gggcgtcgat
     3181 gatctccgcg gcagtaaagg gagaagaagc ggcgctattc tgcacacgcg gccaggcaaa
     3241 ccaggaaacg gaagcagcaa tcagaatggc ggcagcaacc gcgagccagc gccaagcggg
     3301 aatccgaatg aaaccggaag aaggcatcgc agcatcgaga ccagcgcgaa tctttttgtg
     3361 gagggcaacg ggggcatttt cgtaaagacc gctgcgctga agagacgagc gcaaagattc
     3421 ctccgccccg agggaagtgg cgcattcgcg gcaggattcg aggtggcgct caaattccgc
     3481 ggcacgcggg gcatccaatt cgccatccag atagccgtgg aggaccgtgc gggtaaaatc
     3541 acagctcacg ggaggcctcc tgcggagagg cgtgcgtgag cgtgcgctgg agccgctggc
     3601 gggcgcgggc cagcgcggac attacggtgc cgatggggat cgcggtgatg gcggcaatct
     3661 ccttgtaaga gcagccttcg agctcacgaa ggacaagaac ttcgcggaaa cgtggcggga
     3721 gcgtatcgat tgcacgggtc aaacgctctc gattgtcgcc ggcaatggca agagcctcgg
     3781 gagaagtact gggctgagga tgcaattcct cgtcgaattc cgtcatgagt tccacagggc
     3841 gattcttctc caaccaggta taacaagtat tgcgcacaat ctgcaaaagc caggcgcgcg
     3901 cgtccccccc gtgaaacccc cggaaaaagc ggtaggcgcg gagcatggcg tcctgcgcga
     3961 catcttctga atccgcgcga cttcttagca gccaccgcgc cagattgaag gcggcatcga
     4021 gatgcggcag aacgagctgc tcgaagcgcg cccggtcccg cgcatccaac acgtccgaac
     4081 ccttcccctg gtcaaagcca ctcatggaca cgaaccgcct gcacgaccat tttattccca
     4141 aacacctgtt ggatgtgact attagggaaa agggttggta agcggcccac gcgggaataa
     4201 ctgacggccc caagtggtct ggacaagcga aacacgtccc atatgcggcg cgggctccct
     4261 ggactctctc acccgcgccg cccttccaag tatattggcg gcaacgggcg acagagaatg
     4321 gttataggga ttggtgtctt cagatgccga ggaggtaacc atggcgggcc agcggaatga
     4381 cgaaaccctg tgcgaatcac catcacgatg ggatcgaccg gcgtggattt ctgaggcgca
     4441 tggcttgcgc aggtgcagga gcccgttgcg attgacggag attttcccct gtcagggacg
     4501 ggtgagaaag aagcgagaat tgagggggat ccggagtagt actattggga atcgaaagcc
     4561 ggaatcagca agcacgtcga agcgtattct attgtaacta aatagcttaa agtcatttga
     4621 gcgaccttcc acgcctgaaa aacaggcaac ctgtactaca acccaggtac cccgtcgagt
     4681 ggctctggtg acccattgag gccctcgatg caggtgcgta gtctctgaag gacgggttgg
     4741 gggccgcgcg gggacagcgg ctccgcaagg ggccgtgtgc gagcacccaa cccgccagtt
     4801 ttcttttctg cttccctctg cactccagac cagcgcgtat ggaaggaggg cctgcactcg
     4861 ggcccgcctt ttcttttgca tccagcggta ttcgccttgc attctagagc agccacgtgc
     4921 agagatattt ctataagatg ctctcaattg aatgagttag gagtagcctc gtttgtgaga
     4981 tttgaaggat tcatgcgaca ggtgaaggcc atgaacagca ccgccgcaag ggttattgct
     5041 tgtctgctgg tcgcgctggc gctgcaccag atctctgcat gcgtcgcgcg ggccggtgaa
     5101 ccggcgcttc caaaattgaa aacggagacc cggggagcgt ttgaacgcta cttagaactc
     5161 gcggaagcgc gaaatgaagg agagctgaag cgcggtacgg ccctgctgtg ggtcgatgga
     5221 ctccccgagg agcaacgcgc ggaagcctac gcggcgctga aacgcggcga agtaaagatg
     5281 cagaagctgg aaatcctcga caacgccaag accatcccgt gtccgggcgg aatgattcat
     5341 cactggaccg gggtggtatt cattcccgga gcgaaactcg aagacgtgct gggcgtgctt
     5401 gaggattatg accggcactc ggtgtactac gcaccggacg tcgaacgatc gaagattgaa
     5461 tcgcgagagg gcgatcattt ccgggtcttt cttcggtttc ggcggcaaaa ggtgatcacc
     5521 gtggtgctga acaccgagca cgaagtgcag tacttccgcg atgcgccggg aaaagctcat
     5581 agccgcagct tggcggtgcg cattgcggaa gtcgaaaacg ccggtaagag cgatgaacgc
     5641 gaaaaaacac cgggcgacga tgacggattc atgtggcgga tggaaacgtg gtggcgcatg
     5701 gaagagcgag acggcggcgt ttacgtgcaa agcgaggtgg cttcgctgac gcgggacatt
     5761 cccacggggc tgggctggat gatcaaaccg tttgtgaccg acatcccgaa agagtcgctc
     5821 acgtttacgc tgcaagcgac acggagagca gtcgaagccc gccgcgccag ccgctgattc
     5881 gcggatgcgc cctacggttt ccgtgcagga ggcggtgatt tcttgttggc agcgtccttc
     5941 aacaactccc agacgtcagc ggcattgcga tcccaagtcc acgcgcgagc ggcttgggcc
     6001 gccgcgtcac caacattttt gcgaaggact tcgtccgcat gaagctgttg gatagttccc
     6061 gcaagaccgt gaaaatcatc aaattggcga agaatgaacc cgtccattcc gtcgcgaatc
     6121 agatcggcca tgccggcatg catggaagta attacgggca ggccgcaagc catggcttcc
     6181 gcgacgggca agccgaacga atcttcacgg ctcgggctga cgtagagatc agcggccgca
     6241 tagaaatcga gaacattttc gcgagacggt tcaaaacggc agcgctccga aatgccaagc
     6301 gattttgccg tttcacggaa agagtccgga gaatcgtccc cggccgcgat cacgagagtt
     6361 ggcaattctc gaagaatgct catggcgcga agaacggttt caagcccttt gacgcgccaa
     6421 tcgtttccga tcaggagaag cacgaaatca gtttcctgaa tcgcgcggcg ccgccttgct
     6481 tcagcacgca gcgcgagccg cgcggaaggt gaaaactgcg ccgtgttaac gccgttgggt
     6541 atgacgcgga catcttgcct tcgaaaatag tcggcgagga gcgtggcggt gcgctgcgag
     6601 acggcggcaa gagaaactct cgggtcagcg taaatgcggc gctccattcc ggccagaaga
     6661 gcgtagtagg cgcgccgatg gagccgccga agcagactgg gccttgccga atcgagtttc
     6721 tcttcgtgcg caagttcctg gaggcgatgg aagagcgcgt gaacgatgac aacatcggcg
     6781 tccagacagt taattccagg agaaacgaca aggtcgaacc gaagtccatg gacccagcga
     6841 tcccacgcgc gacatattga attcaggaaa agccaggcga gaaattgcag gagatgagga
     6901 ccggggatgg aagaaacttt atgccagatg atcgccccgg agttctgagc gtgtgtaaca
     6961 cctggttgag aaagcgcgag gtcttcaacg cgctgggcat agagatgaat ctcgcaatgc
     7021 tccttgcgcg cgaggcgttc cagcagttcg gcaagcgcgc gctccgtgcc atgccggcgg
     7081 tcaacgaagg gtgaaacggc ggcgaggcgc aagtcagcgc atccgtatcc gtggatcata
     7141 cattggggaa agctgagcgg tggcggcgaa ctgcgcggaa accgcgaggg tggggagccg
     7201 gaacaaaacg cccaggagaa gccaaagata ggcgttcaag acaaaatctt cgtaagcctg
     7261 gattccgcta aacgtcatcg ggagaaacag aagcccggca taccagaaga tgacgaatgc
     7321 cagtggaaac cagggcgaac ctctcagttt tttcacgact cgccaggctg cgaacaggat
     7381 cgcaaagctc atgacaaacc agagcacgag gccgccgatc cccatctcaa taacgagtgt
     7441 gccgaagcca ctctccacgc ctgtgaccgg aggttttgcg ccaaagatgc gcgcgacata
     7501 ttgagtgccg agggaagttg tgccaatgcc gtagccgtag atccaacgct cataaccgaa
     7561 cgccccgagg aaattcgtca gcgggtagtc ccaggtccgg tgctggagct cgctcgcgct
     7621 gctactgggc gaaagcgtct ctgtgtaaaa cgcgacgcga gagaggagcg cttccggata
     7681 agttgcaagc aagactacga tgccaagagc gatgcccagg gctgtgcgct ggacactcct
     7741 taggacccgc atgacctcgc gctgacgcca cggcgccccc cagacgaacg cggcactgcc
     7801 aacgagcgcc gaaccggagc tccaaaggac gacccctcgt gaagaacaga gcgcgaggcc
     7861 cgcaacggtg agagcgagaa caatgaaggc aagagccctg ccgcgtttgt ggcggaggag
     7921 aaggtaacca ctaaaaccaa acacgagcag ccaggttacc agaagaaaat tcccgaaacg
     7981 gccggtactc acgaacaccg atgtgggacg gtacatgatt agaccagaga tgggcgagac
     8041 tctatagagg gtgctcaagt cgcgaatgtc tgcggcaatg atttccggat tcaaaaacgt
     8101 atgcccgatg atggcctgcg ctatccccag ggaaataatg atcagtgcaa gaatcatgtt
     8161 gacaaaaaag aagcggcgca attcggcctc ggaattgagc aaggcgtatc ccacaaagag
     8221 caacggaacg tagtagaaga agagcttcaa gcccagaacg ccgtacatca gatttgtaga
     8281 cgcgggatta aagacctgca tcactccaaa ccagacaaaa ataagaaccg gcacgagaaa
     8341 gggagggcgg aaggatgtga cttcttttcg ccggtaggcc agaaaaaagg agagataaac
     8401 gacggcagcc aaaacgtcct tggcgaaata gatcgccatg ttgttgccca ggtattttct
     8461 tgcaaaatcc tcgaacaaga gccaggtcaa gaagaaatat acgccgttgc gccagttgtt
     8521 gagcatggcg acgatgaatg cgcaacccac gaaggccatc gcagcgaaag cgaggccagt
     8581 catgtccccg acgatcacgt agccggcagt cttgtaagct accacaacaa agaaaaccag
     8641 gaagccgaag acgaaaagcg tacttcggcg gaattggtac ggcgaatcct gcgagtcaag
     8701 cctcatcgaa ctgtcccgtg cggcacacgg taattatggc cgagaacggt ctcatccgaa
     8761 agcccaccat ggtctcagtt ctgttccaac cttttacttc caccgcgcgg gatctccctg
     8821 tatgcgatgg cgtgctcttc taccattcgc acacgttgga agagttgacg cgctcgctcc
     8881 cgggccttca agcccattga atcgatagtg gaagcgtcct gcatcactct tttcatgcac
     8941 tcggctagcg cttcggcgtt tcccgcagga aattggagac cgcagtcgcc gacgacttca
     9001 gatagtccgc caatccggga tgcgatcacc agtctgccgc gcatcatctg ttcaatggcg
     9061 gccaatcccg cggtttcctc gcacaccgac ggcatgacga ccacacgaac gtcgcgaagc
     9121 gcatcggcca aggccgcacc tgtgagataa ccggtgatgc gaacgcagga gcccaaacag
     9181 tttgcttcga tgatttcttc aagtttcgga cgttgcggtc cgtccccgat caaccgaacc
     9241 tcgaattcgc atccttcttc cctcaacatc ccggccgctc gaagaaggat cgcaatccct
     9301 ttttcgggga agaaacggcc cacataggca aaacagactt tgccagttgc gttggctgca
     9361 gaagaaggga cagtgtcctt cccaagcgga tcttcgatac catagtagac gacggatgag
     9421 cgtggcagtc cgtggcgctc gaggacgtgg tgcgaaatag caagattcgt cgaaaccctg
     9481 cgacagagcc agaaacgggg aaacattaga agcagctccg ccagactgcg aagacccgaa
     9541 gtttcggatg cccgacaacg caggcatttc gcataattag cggcctggaa gtgtccagga
     9601 cagatggaac cgtccggctg atggaccata agaccgttgg ggcagatggc ttggtagccg
     9661 tggtgctcga cgacgaccgg tttgcgggca agtgaaccga ggaccagcgg cggaagagcg
     9721 gggcccgcaa tgtggatgac gtcggatttt cgaataatgc gccatagttg gagaacgctc
     9781 ggttgacgga cgacgcgaaa tggcaacgag cgatcgtcga aatcgccggc cggggtatgc
     9841 gtcacgagag ttacgtcaaa ttccgaatcg ccctgagggg tgcggagttt gcggagaccg
     9901 ccagctaggg acagcacagc ggactcgact ccaccgatgc tcggggcgaa gaaatgcgaa
//
LOCUS       DASYVO010000419         2882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_81009, whole genome shotgun sequence.
ACCESSION   DASYVO010000419 DASYVO010000000
VERSION     DASYVO010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81009"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..732
                     /locus_tag="VGR03_14030"
     CDS             76..732
                     /locus_tag="VGR03_14030"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEV1995445.1"
                     /translation="MKHRMNGIGKSPLLAASVLICGLLLAGCDDHVIVTHDSNNPVVK
                     GSTWAWKPVAPPKDADRRPVVSRDVILRGETIAREPGANNEIVRQRIRTAIEQTLSSK
                     GLRQVSDPQAADFLVDYHVAVRRHDVKVERVYPGGYPGLVCGPYGCWESWGWGPPEVS
                     YENIRFREGTVVFDFVKQSTKRLVYRAIGQKPVHRDTFNQDEISEFVHHILGKLKTNG
                     "
     gene            828..1724
                     /locus_tag="VGR03_14035"
     CDS             828..1724
                     /locus_tag="VGR03_14035"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1995446.1"
                     /translation="MPRTQQVVLITGASSGFGRLIAETLARKNYQVFATMRNVNGRNA
                     AAAREIRELADRESLSLQTLELDVTDDVSVERAVSEVTTKCGRIDVLVNNAGYGIMDL
                     AESVSVAQAQRQFDTNFYGVLRMNRAVLPAMKRQGSGLLLHVSSGAGRLVIPGMGLYC
                     ASKFALEALAETYRYELASQGIDSVVIEPGAYATPIMAKLERGEDPGRKAGYGEMAQV
                     PEKLQAKIGGSRANPQEIADVVLQIIETPAGQRQLRYRVGPGGPGVQRINALTDEVQA
                     HLLEAFGITEVTKFKKPSSAAQ"
     gene            1800..2279
                     /locus_tag="VGR03_14040"
     CDS             1800..2279
                     /locus_tag="VGR03_14040"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEV1995447.1"
                     /translation="MRLPAAISNKLRCWSFYIFGCYRGTSGRLKYIAEDWSEVRLDLP
                     LSWRTRNYVGTIFGGSIYSAVDPIYMLMLIRRLGPDFLVWDKSATIQFKRPGRETLHA
                     RFLVSDDELEAVRSALETQRSIDRTYAVDLVDGSGIICATVEKIIYIRRRDQRPPTG"
     gene            complement(2276..>2882)
                     /locus_tag="VGR03_14045"
     CDS             complement(2276..>2882)
                     /locus_tag="VGR03_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995448.1"
                     /translation="PHNTVAESNADTDFGSGGGILLLDVTDLGGNTRHLSVGAGKDSI
                     IYVVDRDSMGKFKASTDQIYQEISGQLGGGVFSMPAFFNNTVYYGAVGDALKAFPVTT
                     ARLAAAPSSQSTHHFGYPGTTPSVSANGTTNGIVWAIENSGAILFAYDATDLTKELYD
                     SNQAANSRDHFSGNKFITPMVVNGKVYVGTPTSVAVFGLLP"
BASE COUNT          610 a          798 c          907 g          567 t
ORIGIN      
        1 tgacggccgc aactctccca tgaattcagg gttgaacatg agcaggggac ggtgtccgct
       61 tgggaggacg cgctgatgaa gcatcggatg aatggcattg gaaaatcgcc gctactggcg
      121 gcttccgttt tgatctgcgg gctgctgctc gccggttgcg acgaccacgt catcgtgact
      181 cacgattcga acaaccctgt ggtgaagggg agcacctggg cctggaagcc cgtcgccccg
      241 cccaaagacg cggaccggcg tcccgttgta tcgcgtgacg tgatcttgcg aggagaaacc
      301 atcgcgcgcg aacccggcgc caacaatgaa atcgttcgcc agcgcatccg cacggcaatc
      361 gagcagactc tttcgtccaa gggattgcgg caagtcagcg acccgcaagc tgcggatttc
      421 ctggtggatt atcacgtggc agtgcggcgg cacgacgtca aggtggaacg cgtgtacccg
      481 ggcggatacc cgggcctcgt ttgtggtcct tatggatgct gggaatcgtg gggctggggt
      541 ccgccggaag tcagctacga aaatatccgc ttccgcgaag gcacggttgt ttttgacttc
      601 gtgaagcaat cgacgaagcg gctggtgtat cgcgcgattg ggcagaagcc ggtgcaccga
      661 gacacgttca accaggatga gatttcggag ttcgttcacc acatcctcgg aaagttgaaa
      721 accaacgggt aggctttcag cggctatcct tggctcctga tcgcgccagc gcgaaataaa
      781 atcagcccta tttacgtccg ggcgcttcct ctctatcctc ttacccaatg cctaggacac
      841 aacaagttgt tctcatcacc ggcgcgagca gcggcttcgg gcggctgatc gccgaaacgc
      901 tggcgcgcaa gaattatcag gtcttcgcca cgatgcgcaa cgtgaacggc cgcaacgcgg
      961 cagcggcgcg tgaaatccgg gaacttgccg accgcgaatc cctctccttg caaaccctgg
     1021 agctcgacgt caccgatgac gtttcagtgg aacgcgccgt aagcgaagtg accacgaagt
     1081 gcggccgcat cgacgtgctg gtgaacaacg cgggctacgg gatcatggac ctggcggagt
     1141 ccgtgtcggt ggcgcaggct cagcggcagt ttgacacgaa tttttacggc gtcttgcgga
     1201 tgaatcgagc ggtcctccca gccatgaaac gccaggggag cggattgctg ctgcacgtaa
     1261 gcagcggagc gggacgcctg gtgattcctg ggatgggctt gtattgcgcg agcaagtttg
     1321 cgctggaagc gctggcggaa acgtatcgct acgaactggc ttcccaggga atcgactccg
     1381 tggtgatcga gccgggcgcc tatgccacac ccatcatggc gaaactagaa cggggagaag
     1441 accccggccg caaggccgga tacggtgaga tggcacaagt tcccgaaaaa ctgcaggcga
     1501 aaattggagg ctcgcgggcc aacccgcagg aaatcgcgga tgtcgtgctg caaatcatcg
     1561 aaacgcccgc cggccagcgg caactccgtt atcgtgtggg accgggcggg ccgggagtgc
     1621 agaggatcaa cgcgctcacg gacgaagtgc aagcgcacct cctggaggcc tttggcatta
     1681 ccgaggtgac gaaattcaag aagccgtcga gcgcggcgca gtagagcggc tctgcgaaca
     1741 cttgacacga agttagccgc gtagcgtcag agattcggcg ggatcatttt ccataatcca
     1801 tgcgattgcc tgcggccatt tcgaacaagc tccggtgctg gtcgttttat atctttggat
     1861 gttaccgggg aactagcgga agactgaaat acatcgcaga agactggagc gaagtgcggc
     1921 tggatttgcc gctctcctgg cgcacgcgca actacgtcgg aacgattttc ggcggcagca
     1981 tttattccgc cgtcgatccg atttacatgc tgatgctgat tcgccggctg ggtccggatt
     2041 ttctcgtgtg ggacaaatca gccaccatcc aattcaagag gccgggccgc gaaactctgc
     2101 atgcccgatt tttagtgagc gacgacgaat tggaagcggt gcgctccgcg ctggagacgc
     2161 agcggtctat cgaccgaacc tacgcggttg atctagtaga cggctcggga atcatttgcg
     2221 ccacggtgga aaaaatcatt tacatccggc ggcgcgatca gcgcccgcca acaggttaag
     2281 gcaacaagcc gaataccgcg acgcttgtgg gcgtgcccac gtagactttg ccattgacga
     2341 ccatcggcgt gatgaacttg tttccgctga agtggtcgcg gctgttggcg gcctggttgg
     2401 agtcgtagag ttccttggtg agatcggtgg cgtcgtaagc aaaaagaatg gcgccactgt
     2461 tctcgatggc ccagacgatt ccgttggttg tcccgttggc agagacgctt ggcgtcgtcc
     2521 caggatatcc gaaatgatgc gtgctctgcg aggaaggagc cgccgcgagt ctcgcggtgg
     2581 tgacgggaaa agctttcagt gcatcaccaa ccgctccgta gtacacggtg ttgttaaaaa
     2641 acgcgggcat ggagaaaacg ccgccgccca actggccgga aatctcctga taaatctggt
     2701 cggtgctggc cttgaacttg cccatggaat cgcggtccac gacgtagatg atcgagtctt
     2761 tgcccgcgcc gacggaaaga tgccgcgtgt tgccgcccaa atccgtaacg tcgaggagca
     2821 agattcctcc gccggaccca aagtcggtgt cggcgttcga ttcggcgacg gtgttgtgcg
     2881 gg
//
LOCUS       DASYVO010000420        15506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_40173, whole genome shotgun sequence.
ACCESSION   DASYVO010000420 DASYVO010000000
VERSION     DASYVO010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 15506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15506
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40173"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VGR03_14050"
     CDS             complement(<1..390)
                     /locus_tag="VGR03_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995449.1"
                     /translation="MNRRFLLYVLFVCAAGAATLGYVFQRRLSTAAEFDHQLKLAKDE
                     AARIREQVILPTEKRVPAGENFTAALQKFGLSAGEAASASAAAQDAFNLRQVRAGNTI
                     TVGRSVEGTLREIDYKIDPDRMLKIVPE"
     gene            complement(707..1759)
                     /locus_tag="VGR03_14055"
     CDS             complement(707..1759)
                     /locus_tag="VGR03_14055"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEV1995450.1"
                     /translation="MSAVFALPLPAAPRAGTPVKRTHKYVTRYRGVPTFADSSSADDG
                     AYDDPIVRAAAVSALGRYNGAVVAVDPNTGRILTVVNQKIAFGDGYIPCSTIKPTIAL
                     AALEENVITRDTMLKVGRRKYMNLTEAMAHSNNVFFEQLGSRMGFETVSKYGRLLGLG
                     ELAGYNLPDEHPGSFPTVPPAYGGVARMSSFGEGIQVTPLQLAALSAAFANGGTLYYL
                     QYPHSQEEVENFTPRVKRELNIGTILPEIREGLLAAVLYGTGKSSFDRGGDEMPLGKT
                     GTCDDHTTPSKIGWFLTYADDAHPKIAFAVLLRGNTRSVKGPTAAVVAGRIYRKLREQ
                     NYFADSAHAVALNTGN"
     gene            complement(2171..2959)
                     /locus_tag="VGR03_14060"
     CDS             complement(2171..2959)
                     /locus_tag="VGR03_14060"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1995451.1"
                     /translation="MSSHNEVVRESFTTQAKAFAANPWVTDEERMRRLVAAARLKGDE
                     RVLDIATGPGYIAEAFARGAREVIGVDLTAAMLAIGEVRTKERGVSNVSFRVGDVQNL
                     PFEKEEFDVVVSRLALHHMQNPAQVVREMTRVCRLGGTVLVEDIYGSEHPERAAYQDH
                     WERLRDPSHVRTLPISELLHLFSEAGLETDHVSTFEDLCPEVERWLATTKTPAADAAE
                     VQRLLEEDRLHDLSGTRPFQDATGHLHFHARTATLTGRRFRLLT"
     gene            complement(3123..4247)
                     /gene="recA"
                     /locus_tag="VGR03_14065"
     CDS             complement(3123..4247)
                     /gene="recA"
                     /locus_tag="VGR03_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV1995452.1"
                     /translation="MTEEKEKLLAAAISQIEKDHGKGAIMRLGSRDILVPVSVIPSGC
                     LSIDAALGVGGFPRGRVIEIYGPESGGKTTMTLHVIAEAQKLGGQAAFIDAEHALDPG
                     YARKLGVDVDNLLVSQPDNGEQALEIAETLIRSGGVDIVVVDSVAALVPKAELEGDMG
                     DPQMGLQARLMSQALRKLTGIVSKSRTCLIFINQIREKIGVMFGNPETTTGGRALKFY
                     ASMRVDIRRIQAIKEGDKVVGSRTRAKVVKNKVAAPFREAEFDIVYGEGISREGDLID
                     LGVEKGVLEKSGTWISFGGERMGQGRENARTFLKENKDIRDKLENTLRKKLGIPIPGN
                     SNEAGAGPNGHAAPAQAEKPPMKASAAVAGADASKARTTR"
     gene            complement(4861..5724)
                     /locus_tag="VGR03_14070"
     CDS             complement(4861..5724)
                     /locus_tag="VGR03_14070"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: protein motif:HMM:NF004474.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEV1995453.1"
                     /translation="MSIDSIKTVAVLGAGTMGNGIAHVFARSGYKVILRDVEQRFLDR
                     GMETIGKNLDREIKKGKIAEAEKGIVLGRLQPVTDMAAIGAADFVVEAVPEKMEIKRT
                     VLGEADRILRPEVIMASNTSSISMTTLAALTKRPDRFVGMHFMNPVPVMVLVEVIRAL
                     QTSDETFEATMELAKKLGKTPVAVNDAPGFVSNRVLMPLINEAAYCVMEGVATAEAVD
                     AVMKLGMDHPMGPLELADFIGLDVCVDIMHVLHEGLGDPKYRACPLLKKYVAAGWLGR
                     KSGRGFYSYPA"
     gene            complement(5854..6297)
                     /locus_tag="VGR03_14075"
     CDS             complement(5854..6297)
                     /locus_tag="VGR03_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995454.1"
                     /translation="MPDVHVERIQYPPEHYRVFFFAHWVASALTYRRPTLLFITEWGV
                     WPSSENWHLYYKLRQTYDDNRLLHDAPGHLFLEHEGEDLASFLQLSMLNGWGGYVLTE
                     ANYVNAFFSHDEYIDFFAQDSGNLADIRKMLGPSQPTADGTVTTG"
     gene            complement(6538..7083)
                     /locus_tag="VGR03_14080"
     CDS             complement(6538..7083)
                     /locus_tag="VGR03_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995455.1"
                     /translation="MLARERVQLVETFTYEFRQPYHTAKKMPQGETREFISRMQAEAR
                     RLAYRAIRDVQSDLQKQGLKLMRCGLVLASGKPLPALDRILASHALIHTADGELFREA
                     LLHASARCGLRDFRIKEKELLDRAGQVLRLKSGALMGQVTALGKPLGSPWSQDEKFAT
                     LAAWLAMLAPLRNSRETRKSR"
     gene            complement(7187..7717)
                     /locus_tag="VGR03_14085"
     CDS             complement(7187..7717)
                     /locus_tag="VGR03_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995456.1"
                     /translation="MSSKSLPQGSLVLLWAGENPALHAALLDELQAADIPFSDKTLGD
                     DEVAPTADPLPIDWKPRFGFEVAVLSTDLAAAKEMLEKLLDEEPADVEIPAQDDVAAA
                     EPPLMVTTETHPTVEVWNGNEDRVAQFLTAAMQENEIPIHLENPGEQTRIYVSAGNEK
                     RAREIVREIVEGAPPR"
     gene            complement(7906..8583)
                     /locus_tag="VGR03_14090"
     CDS             complement(7906..8583)
                     /locus_tag="VGR03_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995457.1"
                     /translation="MGASGDAGAQAEISEDPFCSFWRGDDPRIHAELCELLNEEGIPH
                     KTIRREDHLFNLNSKSGFQIGIPFSQFEKAEAAIKDAYGTEEEQQDAVRLLPYGKGYA
                     AGLRGAFPWRPVLKGFTRADGSQGDEPESVAAPVPEAFDVEDKSENCRPKWDPANWNP
                     EDATLGVWSSEQFYPGEIIELALRENQIHVRFEKAERKNAIFVLPEDEIRAREIVREI
                     VEGAPPE"
     gene            complement(8867..9208)
                     /locus_tag="VGR03_14095"
     CDS             complement(8867..9208)
                     /locus_tag="VGR03_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995458.1"
                     /translation="MFLDVWQIKELWAHFSDVWQIKDLVESGIDSKGFRVGVSGADLE
                     VWIPKKLGELLIESKGVTDALASDNVEELSDSPDVWQVKGLEEEERELGNGALAGGAG
                     RGTIPPDMVTL"
     gene            9412..10008
                     /locus_tag="VGR03_14100"
     CDS             9412..10008
                     /locus_tag="VGR03_14100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV1995459.1"
                     /translation="MPGPNLFFSTMMSDEAKTIARGLQRRDPDLLDRLIEQYQYRLFR
                     YLVYVTGNKERAEDFFQETWIRVLERGHQYDGKSKFEAWLFAIARHLVIDWQRSKKPQ
                     SLDSLTDPEQGHPLQLANEKEPSPLHQVLSQEKEENVQASLQKIPAIYREVLVLRFQE
                     ELQIDEMAGVLSVPLSTVKSRLYRGLEALRGALQGGAV"
     gene            10094..10582
                     /locus_tag="VGR03_14105"
     CDS             10094..10582
                     /locus_tag="VGR03_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995460.1"
                     /translation="MSADFHTRAEQLIAQERVESISQAERDWLAAHLQECAHCAELAR
                     QTDQALRSMRTAAISLPSGLASRTQFRVRLRAQELREREPKRRALWLACGVSWIFGVA
                     SAPYVWNLFQWIGQRTGVPKLVWEVGFGLWWTIPALFAAAVLLMESSRKGEQADWINQ
                     GS"
     gene            10611..11252
                     /locus_tag="VGR03_14110"
     CDS             10611..11252
                     /locus_tag="VGR03_14110"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV1995461.1"
                     /translation="MSDYPQRMTDSFNERKRLIRLRRKKENLRFWEEFGIIPRWLMVL
                     VAVLYVIVLVTAITVNLAARNNPNGNDMFPPDLRNNPALASLALAGIVTAASLSLAII
                     IFFVAYVNRDASRRGMSAALWTILVIILLPAWGFIGFVIYFLMREPLPYPCPQCASTV
                     GARFNFCPNCKCNLQPSCPQCKREVAETDKFCPYCGNDLKAAGDAEPALQRSI"
     gene            complement(11271..12461)
                     /locus_tag="VGR03_14115"
     CDS             complement(11271..12461)
                     /locus_tag="VGR03_14115"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEV1995462.1"
                     /translation="MSTETPVILSAVRSPIGKFQGGLSGFSAPELGGKAVAEAIRRAG
                     LDAKQIDEAILGNVLQAGLGQNPARQAALKGGCDARVAAMTINKVCGSGLKAVALAAQ
                     AVQLGESEIVVAGGMESMSNCPYLLPQARTGYRIGDQKVVDSMIADGLWDAYENFHMG
                     MTGELVAEKYGITREEQDKFAVESHQKAVRARKSCFFEAQILPIEVPQKKGDPIIIKF
                     DESPREDASMEALAKLKPAFKKDGTVTAGNAPGTNDGAAALIVTSERNASRLGKQPIA
                     RIVAQAVSGVEPKWVMMAPVDAVEKILTKTGWDRDKDVDLVELNEAFAVAAIAVTRQL
                     KLNPEKVNVNGGAVALGHPIGASGARILVTLLYELQRRNLKRGIAALCLGGGNAVALA
                     VERF"
     gene            complement(12574..13107)
                     /locus_tag="VGR03_14120"
     CDS             complement(12574..13107)
                     /locus_tag="VGR03_14120"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV1995463.1"
                     /translation="MTIGQMMLGEFDQEMQNTRKTLERCPDDKWNWKPHDKSGTVGWL
                     AGHIATMPGWTAVTLTTDQLDYAPVGGPAYQPPKTDNRKELLAVFDKNVAEARAALST
                     ASDQEMMKPWKLLAGGKEILTMPRVACLRGMIFNHIIHHRAQLTVYFRLLGIPVPGLY
                     GPSADEGQPGAAEAAAN"
     gene            13336..13884
                     /locus_tag="VGR03_14125"
     CDS             13336..13884
                     /locus_tag="VGR03_14125"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 domain-containing protein"
                     /protein_id="HEV1995464.1"
                     /translation="MAHQFTTSYLGDSIDVFHYYKKLGERAMTQCPEEALFTTLDAES
                     NSIAIIVKHIAGNMRSRWTDFLTTDGEKPDRNRDTEFEAPPKTRAELMEMWERGWNGV
                     FDALEPLSDADLTRTITIRTEPHSVMQAVNRQLAHYSYHVGQIVYLARHFAGDKWQTL
                     TVPKKKSAELNARVAAGEISQR"
     gene            14482..>15506
                     /locus_tag="VGR03_14130"
     CDS             14482..>15506
                     /locus_tag="VGR03_14130"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEV1995465.1"
                     /translation="MATLFEMEPLGESGLKLNDAQKRAITHGEGPLLVIAGAGTGKTR
                     VITERIRHLLQSDESLSGENILGLTFTKKAAGEMKTRVVKATGERGKAVTLATFHSFC
                     ETLLAEADPQRVMLDKFDHWILLRRNLQRLRLEKYRRLADPGQFLNDFVEFFSRCQDE
                     LVSCEDYQHYAAGLAARLEAEREALDEDTLAERLETVALQQELARAYRASEELLREKN
                     RVSFGSLITGAVGLLEQDAQLRQTLQKKYHYILVDEFQDTNIAQLRLLELLAAPTRNI
                     VVVGDNDQAIYRFRGASFGSFKLFLERFAGWNEGQDSTPFRVALTENYRSTPNILRVA
                     MQVIGQNA"
BASE COUNT         3226 a         4776 c         4244 g         3260 t
ORIGIN      
        1 ctccgggacg atcttcagca tgcggtcggg gtcgatcttg taatcgatct cgcgcagtgt
       61 tccttccacg gagcgcccca ccgtgatggt attcccggcg cggacctggc ggagattaaa
      121 agcgtcctga gcggcggccg aggcgcttgc tgcttctccg gcgctcaagc cgaatttctg
      181 aagcgcggct gtgaaattct ctcccgcagg aactctcttt tccgtcggca ggatgacctg
      241 ttcgcggatg cgcgccgctt catcctttgc cagcttcaac tggtgatcga attccgccgc
      301 ggtggacagc cgtcgctgga acacataccc cagagttgca gctccagccg cacacacgaa
      361 cagcacgtag agcaaaaaac gacgattcac gattccccca atgccactcg ctgctgccac
      421 ccgtatcagg cggaacttcc ctggacgagt cgctgccgcc atcttgacag attaatgccc
      481 ctcacacttt tgtcgcaaat tgaggttggc gtcaaacgcg attcgcttcg cgtgcgctca
      541 atcgtccaac tggttgatcc agctgaaacg ggcgggagcg cgcatccagc atgcacatca
      601 ggccgcgcct ccatgggcca aagccggatg cgccgcccga aaagagaaac gctgcggaga
      661 ctctccgcag cgcaagaaaa ccagtactaa ataaactgca gttgagtcag ttgccggtgt
      721 tgagggccac ggcgtgagcg gagtccgcaa aataattctg ttcgcggagc ttgcggtaga
      781 ttcggcctgc aacaacggca gcggtgggac ccttcacgct gcgcgtgttc ccacgcagga
      841 gcactgcgaa cgcgattttc gggtgcgcgt cgtcggcgta ggtgaggaac cagccgattt
      901 tcgaaggcgt ggtgtgatcg tcgcaagtcc ccgtcttgcc gagcggcatc tcatcacccc
      961 cccggtcgaa actcgatttg ccggtgccat acaggaccgc ggcgagcagg ccttcgcgga
     1021 tctccgggag aatcgtgccg atgtttaatt cgcgcttcac gcgcggcgtg aaattctcaa
     1081 cttcttcctg gctgtggggg tactgcaaat aataaagtgt cccgccattc gcgaacgctg
     1141 cggagagcgc cgcgagttgc agcggggtca cctgaatccc ttcgccaaag ctggacatcc
     1201 gcgcgactcc gccataggca ggcggcacgg tcgggaacga acctggatgt tcgtcaggca
     1261 ggttgtagcc ggcgagttct cccaggccga gaaggcggcc gtacttggaa actgtctcga
     1321 atcccatgcg tgacccaagc tgctcaaaaa aaacgttgtt cgagtgcgcc atggcttccg
     1381 tcaagttcat gtacttgcgg cgccccacct tgagcatggt gtcgcgcgtg atgacgtttt
     1441 cttcgagggc ggcaagtgcg atggtcggct tgatcgtcga gcaaggaatg tagccatcgc
     1501 caaacgcgat tttttgattc acaactgtga ggatgcggcc ggtgttcgga tcaaccgcga
     1561 cgaccgctcc gttgtaacgg cccagcgcgg aaaccgcagc cgcgcgaacg atgggatcat
     1621 cgtatgcgcc gtcgtcggcg ctggaagaat ccgcaaacgt tgggactccg cgataacgcg
     1681 tgacgtattt atgagtgcgc tttaccggcg ttcccgcgcg tggcgccgcg ggcagaggca
     1741 aggcgaagac tgctgacatc agtattgcga gagtggcttt tccgaactgc ttcaccgttc
     1801 tcaccttttc attcagggtg accgccaacc ggtcaatccg ccctagcctg ccccaagcca
     1861 ataaaccatc ggggtcagag ttttacctca cgctgtccgc agggtcaaca aacattcaca
     1921 gcacctgttc gttgccgcta ccggctgctt gccgccagcg gaagggctgg agagagttat
     1981 tgcatcccac ctgccaatta aggacaccct tcatttgatg cgacttacgg gtgtcgcgtt
     2041 tggcagtctc tccacccaag agaaactgtt tctctcagga tgagacatat tttggccctt
     2101 aaaccgttct cacgcaatag ccgtcccggc ccagtacgag gctatgtgct ttgttttgaa
     2161 tagctgaccg tcaggtcagg agcctgaacc ttcgtccggt tagggtggcc gtcctggcgt
     2221 ggaagtgcag gtgtcctgtc gcatcctgaa acggccgggt accgctcagg tcgtggagac
     2281 ggtcttcttc gagcagacgt tgcacttcag ccgcgtccgc cgcaggcgtt ttcgtggtgg
     2341 ccagccatcg ctccacttct gggcagagat cttcaaaggt tgaaacgtga tcggtttcca
     2401 gccccgcctc gctgaaaaga tgcagcaact cgctgatcgg cagtgtccga acgtgcgaag
     2461 gatcacgcaa tctttcccag tggtcctgat acgcggcccg ctcgggatgc tcgctgccgt
     2521 agatatcttc aaccagcacg gtaccgccaa gacggcatac ccgcgtcatt tctcgaacaa
     2581 cttgcgcggg attctgcatg tgatgaagcg cgaggcggct taccacaacg tcaaactctt
     2641 ccttttcgaa gggtaggttc tgcacatccc cgactcgaaa cgatacgttt gaaacccccc
     2701 gctccttagt gcgcacttcg ccaatcgcca gcatcgcggc agtcaaatcg acgcctatca
     2761 cttctctcgc gccccgcgca aatgcttcgg cgatgtaccc cggcccggtg gcgatatcga
     2821 gcacgcgctc gtcgcccttc agccgtgccg ctgccaccag cctgcgcatc cgttcttcgt
     2881 ccgttaccca cggattggct gcaaacgctt tcgcctgcgt ggtgaaggat tcgcgaacaa
     2941 cttcgttatg gctcgacaaa gttccccctg atgactgcga actggaagtt taacagcgtg
     3001 cctcgcatgc gcaaacaatc cctgttgcgg cctgaaagca ggcaccacgc gaagagtgaa
     3061 aaaagacctg gcccttctcc acgtaggcag aagggccaat cttcgtgatc ggacggttgc
     3121 ggttagcgcg tagtgcgagc cttggacgcg tccgcgcccg cgactgctgc cgaagccttc
     3181 atgggcggct tctccgcctg tgcaggggca gcgtggccgt tcggcccggc cccagcctcg
     3241 ttcgaattac ccggtattgg gattcctagc ttcttcctca gcgtgttttc cagcttgtcg
     3301 cggatgtcct tgttttcctt aaggaaggtg cgggcgtttt cgcggccctg gcccatgcgc
     3361 tcgccgccga agctgatcca cgtgccgctc ttttccagca cgcccttttc aacgcccaga
     3421 tcgatcaggt cgccttcgcg tgagataccc tctccgtaga cgatgtcgaa ttcggcctcg
     3481 cggaagggcg cggcgacttt gttcttcacc actttcgcgc gcgtgcgcga gccgacaacc
     3541 ttatcgcctt ccttgatggc ctgaatgcgc cgaatatcca cacgcatgga cgcgtagaac
     3601 ttcagcgcgc gcccgcccgt ggtggtctct ggattgccga acatcacccc aatcttctcg
     3661 cgaatctggt tgatgaaaat caggcaggtg cgcgactttg aaacgatgcc ggtgagcttg
     3721 cgcagcgctt gcgacatcaa tcgcgcttgc aaacccatct gcgggtcgcc catgtcgcct
     3781 tcgagttcgg ccttgggaac cagcgcggcg acggagtcca ccacgacgat gtccacgcca
     3841 ccggaacgga tcagcgtttc ggcgatttcg agcgcctgct cgccgttatc gggctgcgag
     3901 accagcaggt tgtccacgtc cacgccgagc ttccgtgcat agcccggatc gagcgcgtgc
     3961 tccgcatcaa taaacgccgc ctgcccgccg agcttctggg cttcggcgat aacgtgcagc
     4021 gtcatggtgg tcttgccgcc ggattccggg ccgtagattt cgatgacgcg cccgcggggg
     4081 aaaccgccaa cacccagcgc tgcgtcgatc gagagacatc cgctggggat tacactgacg
     4141 ggcaccagaa tatccctgct gcccagacgc atgatcgcgc ctttaccgtg gtccttttca
     4201 atctgcgata tcgctgctgc cagcaatttt tctttttctt ctgtcatgtc acacctcggt
     4261 gaaggaaagt aggaaacttg gcctgcgggg ccgatggcct gggttccgcg ccgggaaagc
     4321 gcatcgccct tgtccgggga cgtaacgaag tgtggagctt accactcgat tcatcttcgc
     4381 gctattccct ttttcgagcc gccaccatcc ggatttgtca tcctgaggcc atccgaaaag
     4441 gatggccgaa ggacctcaac tcccaatagt gccgaccccg aaccgcctaa cgccacatca
     4501 ccgctgacaa ccgtgctccg cgtcgtggtc tctgagccgc gtctgagccg tctgctcgcg
     4561 ctgatccaac ttcgcccgtt cgggcggagt cagatgccgc agccgggata ggttccgccc
     4621 ccgggtgcga gctttggaga gctcctcttc gagtctctca cgttccagac agcttagaac
     4681 accgttcgaa aaaccacgca tgaagcacct gttgaaaacg acgaggagca aagtagcacc
     4741 atggcgaaca aaaagcaaac actatttttc gccttatgtt cgcccatgcg tcttggagtg
     4801 cggcggcttg ccgccgcttt tccgactgct cccaaccacc cggccaatcc tcgatgcaac
     4861 ctaagcagga tacgagtaga agccccggcc cgatttccgc cccagccacc ccgcagccac
     4921 gtatttcttc agcagcggac acgcacggta cttcgggtca cccagccctt cgtgcagcac
     4981 gtgcatgatg tccacgcaga cgtcgagccc aatgaaatcc gccagctcca gcggccccat
     5041 gggatgatcc atccctagtt tcatcaccgc gtcgacagct tccgccgtgg ccacgccttc
     5101 catcacgcag taagcggctt cattgatcag cggcatcagc acgcggttcg acacaaaccc
     5161 cggcgcatcg ttcaccgcca ccggcgtctt gccaagtttt ttcgccaact ccatcgtcgc
     5221 ctcgaacgtc tcgtcgctcg tttgcagcgc ccgaatcact tcgaccagca ccatcaccgg
     5281 caccggattc atgaagtgca tccccacgaa gcggtcgggc cgcttcgtca gtgccgccag
     5341 tgtagtcatg gagatcgatg acgtattgct cgccatgatg acttcggggc gcagtatccg
     5401 gtccgcttcg ccaagcaccg tccgcttaat ctccattttc tccggcaccg cttcgaccac
     5461 gaaatccgcc gccccaatcg cagccatatc cgtaacgggc tgcagccggc caagcacaat
     5521 tcccttctcc gcctccgcaa tcttcccctt cttgatctcc cgatccagat tcttcccgat
     5581 cgtctccatg ccccggtcaa gaaagcgctg ctccacgtcg cgcaaaatca ctttgtagcc
     5641 cgaccgcgca aacacatgcg cgatcccatt ccccatcgtc cccgcgccca gcaccgccac
     5701 cgttttaatc gaatcaatcg acacggctaa aatctccgaa acgcaagtcc taatgaaagt
     5761 aaagaaagcc aagaatgcac tccgctcaaa acaacgtcaa gctctggagc tttaagcaga
     5821 gggccagttt ccgattatca gacttatcgg gagttaccct gtcgttaccg tgccatcggc
     5881 ggttggttgg cttggtccga gcatcttgcg gatgtcggct aggttcccgg aatcttgggc
     5941 aaagaagtca atgtattcgt cgtggctgaa aaacgcgttt acgtagttag cttccgtcaa
     6001 aacgtagcct ccccagccgt tcagcatgga cagttgcagg aaggatgcca ggtcttcccc
     6061 ttcgtgttcc agaaacaggt gccccggcgc gtcgtggagc aggcgattgt cgtcatacgt
     6121 ctgccgcagc ttgtaataca aatgccagtt ctcgctgctc ggccaaacgc cccactcggt
     6181 gataaagagc agggttggcc tgcgatacgt caatgcagag gcgacccagt gagcaaaaaa
     6241 gaaaactcga tagtgctcgg gcgggtattg gatgcgttca acgtgtacat ccggcatcag
     6301 gtccggcttc cgtcgttgtc ggtcggatag ccacgtctcg cattcctctt tcgtatagaa
     6361 gcgcaattat cccccctgcg aacagttcag tattaggccg aaactctcac gccaagtaag
     6421 tatcgaaggc cccattctga agaagaaatt gaaccgaatc aagcacgccg ggaaacgtgc
     6481 taccgacact taatcggagt caatgcctgg ttaacgttta aatcggaaac tgacccgcta
     6541 ccgagacttg cgcgtctcgc gactattcct gagaggggcg agcatggcga gccacgcggc
     6601 gagcgtggcg aacttttcgt cctgggacca cggagaacca agaggcttgc caagtgcagt
     6661 aacttggccc atcagagcac cgctcttgag acggagaacc tgtccggcgc ggtccagaag
     6721 ttctttctct ttgatcctga aatcgcggag gccgcagcga gcgctcgcat ggagaagagc
     6781 ctcgcgaaat agttcaccgt ccgccgtgtg aatcagcgca tgcgaagcga ggatcctatc
     6841 caacgcggga agcggcttcc cggaggcaag gacaaggccg catcgcatga gcttcagccc
     6901 ctgtttttgc aagtcggatt gaacgtcgcg gatcgcacgg taagcaagcc gccttgcctc
     6961 ggcctgcatc cgcgagatga attcgcgggt ttcaccttgc ggcatctttt tcgcggtgtg
     7021 atacggctgg cggaattcat atgtgaaagt ttcgacgagt tggactcttt cccgtgcgag
     7081 caccgtaggt gagcctctat cgacgcatac tgcgacaagc gcggtccacc ccgaatgcgc
     7141 gcgaaagcca agcgctactt gtttcgaatg ggccactgag aaaaacctac ctcggcggcg
     7201 cgccttccac aatttctcgc acgatttccc gcgcccgctt ttcattcccc gcggaaacat
     7261 aaattcgcgt ctgctccccg ggattctcca gatgaattgg aatttcgttt tcctgcatcg
     7321 ccgcggtaag aaattgcgcg acccggtcct cattcccatt ccaaacttcc accgttggat
     7381 gcgtctcggt agtcaccatg agtggcggct ccgctgcggc aacgtcgtcc tgcgctggaa
     7441 tttctacatc ggcgggttct tcatcaagca gcttttccag catctctttc gccgcggcaa
     7501 gatcggtgga aagcaccgcc acctcaaatc caaaccgcgg cttccaatca atcggaagcg
     7561 gatcggccgt cggcgcgact tcatcatccc caagggtctt gtccgaaaat ggaatatcag
     7621 ccgcttgaag ttcatcgagc aacgccgcgt gtaacgcggg attctccccg gcccacagca
     7681 agaccagcga accttgcgga agtgatttgg atgacatggc ttcctcctgt ggaccgaagc
     7741 attcggggcg agcttgccag cgtcggcgca acatcaccta acaaaacccg ccttgacgtg
     7801 cagccgaaaa cggcagaatc gccgacctag tttactactt ccctggacgc atctgcattt
     7861 cgctcggcgt gcgcggcagg ccgcgcgatt cgctaatcca attcttcatt ccggcggcgc
     7921 gccctcgacg atttctctga caatttcacg ggcgcggatt tcgtcttccg gcagaacgaa
     7981 gattgcattc tttctttccg ctttttcaaa gcgcacatga atctgatttt cccgcaacgc
     8041 cagctcgata atctctccgg gataaaattg ctcgctggac cacactccga gcgtggcgtc
     8101 ttccggattc caattcgctg gatcccactt cggccggcag ttttcggatt tatcttccac
     8161 atcgaatgct tcaggaaccg gtgccgccac actctcaggt tcatcgccct ggctgccgtc
     8221 ggcccgggta aaacccttca acacaggtcg ccaggggaaa gcaccgcgta agcccgccgc
     8281 gtatcccttt ccatagggca gaaggcgaac agcatcctgc tgctcttcct ccgttccata
     8341 agcatctttg atcgcagctt ccgccttctc gaattgcgaa aacggaatcc cgatctggaa
     8401 gccactcttg gaattgagat taaagagatg atcctcgcgc cggatggtct tgtggggaat
     8461 gccttcctcg ttgagcagtt cgcagagctc cgcatgaatg cgcgggtcgt caccccgcca
     8521 aaaggagcaa aaaggatcct ccgaaatttc agcctgagca cccgcatcgc ccgaggcgcc
     8581 caatccactc gcggctgcaa gagggtcatg cagcagatca gtatcgcaat cggcgcagcg
     8641 cgtaaaccct cgccgatact cagctttgca ttgcggacag aacatggcag ccggaagtgt
     8701 atctcaacgg tcatggcgcg gggagcgagg ccttcgagga ctatgtccag aagacgtgct
     8761 acgtagccgg ggcaactgct ggtggcaacg gcagtaagcc ttagcctctt aagcgaagct
     8821 ttacagcatc cgttctattt aatccttgac taacttaacg aacgtgctat aatgtgacca
     8881 tgtcaggtgg aatggtcccc ctgccggctc ccccggcgag agcaccattc cctaattctc
     8941 gctcttcttc ctctaaaccc tttacttgcc acacatccgg agaatcgctc aattcctcga
     9001 cattatcgga cgcaagcgcg tctgtaactc ctttcgattc aatcagcagt tcccctaact
     9061 tctttggaat ccacacttcc aaatctgccc ccgaaactcc aactctaaac ccttttgaat
     9121 caatcccgct ctctactaag tcttttattt gccacacatc cgagaaatgc gcccataact
     9181 cctttatttg ccacacatcc aaaaacacag cacttaaagt cctgtgtttg ccacacatcc
     9241 gaaaaaaggg gggtggggag gaattcctgt taaccaggaa tcccaggaag gatatcctcc
     9301 ctagcgcgct gcctgagtct tttcgccact ctcctccgta taccagttag aatgagcgcc
     9361 tcaatctcat gtttgacccc acagcggtcc ggtctctttg tgaccccggc catgcccggt
     9421 ccaaacctgt tcttctccac gatgatgagc gatgaagcaa agaccatcgc acgcggcttg
     9481 cagcggcgcg accccgatct gctcgaccgc ctgatcgagc agtatcaata ccgcctgttc
     9541 cgttatctgg tatatgtcac gggaaacaaa gagcgcgccg aagacttctt ccaggaaacg
     9601 tggatccgcg tcctcgagcg cggccaccag tacgacggca agtcgaaatt cgaagcctgg
     9661 ctcttcgcca tcgcgcgcca cttggtcatt gactggcagc gctccaagaa accccagagc
     9721 ctcgattcgc tcaccgatcc agaacaaggg caccctctcc aactcgccaa cgaaaaggag
     9781 ccctcgcctc tgcaccaggt cctcagccag gaaaaagaag aaaacgtcca agcatctctc
     9841 cagaaaatcc ccgcgattta tcgcgaggtg cttgtcctgc gcttccagga agaattgcag
     9901 atcgacgaga tggccggcgt gctgagcgtt cccctttcca cggtgaagtc gcgcctgtat
     9961 cgcggactgg aagcacttcg cggcgcattg cagggaggcg ccgtatgaac cagtcaatac
    10021 acgcccgcgc caaacacgtg aatgctttgg taggggcgca gcatgctgcg ccccagtttg
    10081 aggagatctg caaatgagtg cagactttca cactcgcgcc gaacaattga tcgcccagga
    10141 acgcgtcgaa agcatctcgc aagccgagcg cgattggctc gccgcgcatt tgcaagagtg
    10201 cgcacactgt gcggaactgg cgcgccaaac ggatcaagca ctgcgctcga tgcgcacggc
    10261 ggcaatttcc ctccccagcg gtctcgccag ccgcacacaa tttcgcgtgc gcctgcgcgc
    10321 ccaggaattg cgcgaacgtg aaccgaagcg ccgggcgctt tggctggctt gcggagtgtc
    10381 gtggattttt ggcgtggcca gcgcgccgta tgtgtggaac ttgtttcaat ggattggcca
    10441 gcgcacggga gtgccaaagc tggtctggga agtgggcttc ggcctatggt ggacgattcc
    10501 cgcgctcttt gccgcggccg ttctgctgat ggagagttcg cggaagggcg aacaggccga
    10561 ttggatcaac cagggaagct gaccgggacg agtggagaga gagaaaacga atgtccgact
    10621 atccgcaacg catgaccgat tcattcaacg agagaaagcg gctgattcgt cttcgccgca
    10681 agaaggaaaa tctccgcttc tgggaagagt tcgggatcat tccccgatgg ctgatggtgc
    10741 tggtggccgt gctgtacgtg atcgtcctgg tcacggcgat cacggtcaat ctggcggcca
    10801 ggaataatcc taacggaaat gacatgttcc cgccggacct gcggaacaat ccggctctgg
    10861 cgtcgctggc gctggccgga atcgtcaccg cagcaagtct tagcctcgcg atcattattt
    10921 tcttcgttgc ctacgtgaac cgcgatgcct cgcggcgcgg aatgagcgca gctctttgga
    10981 cgattctggt gattattctc ctgccggcgt ggggtttcat cggtttcgtc atttacttcc
    11041 tgatgcgcga gccgctgccg tatccctgcc cgcaatgcgc atccactgtt ggcgcgcgct
    11101 tcaacttctg ccccaactgc aagtgcaacc tgcagccgtc ctgcccgcag tgcaagcgcg
    11161 aagtcgccga aaccgacaag ttctgcccct actgcggcaa tgacctgaaa gcagctggag
    11221 atgcagagcc agcattgcag cgttcaatct aggctgacgc tcagctgatc tcagaagcgc
    11281 tcgacagcga gggcgacagc gttgccaccg ccgaggcaca gggcggcgat gccacgtttg
    11341 agattgcggc gctgcaattc atagagcagc gtaacgagga tgcgcgcgcc gctcgccccg
    11401 atgggatgac cgagggccac cgccccgcca ttgacattga ctttctcggg attgagcttg
    11461 agctgccgcg tcacggcaat ggcagcgacg gcgaacgctt cgttgagctc gaccagatcg
    11521 acatccttgt cgcggtccca accagttttc gtcaatatct tttccaccgc gtcgaccggc
    11581 gccatcatca cccacttcgg ctcgacgccg ctaacggctt gcgcgacaat ccgcgcgatc
    11641 ggctgtttgc caagtcgcga cgcattccgc tcgctggtaa cgatcaatgc cgccgcgccg
    11701 tcgttcgtgc cgggcgcatt gcccgccgtg accgtgccgt ctttcttgaa cgccggcttc
    11761 aacttcgcga gagcctccat gctcgcgtct tcgcgcggcg attcgtcgaa cttgatgatg
    11821 atcggatcgc cctttttctg cggcacttcg atcggcaaaa tttgcgcttc aaagaagcac
    11881 gatttcctcg cgcgcaccgc tttctggtgg ctttcgacgg cgaacttgtc ctgctcttcg
    11941 cgagtgatcc cgtatttttc ggcgacgagc tccccggtca tgcccatatg aaaattctcg
    12001 taggcgtccc acaatccatc cgcaatcatg gagtccacta ccttttggtc tccaatccgg
    12061 tagccggtgc gcgcctgcgg caggagatac gggcagttgg acatggactc catcccaccc
    12121 gccacgacaa tctccgattc cccaagctgc accgcctgcg ccgccagggc cacagctttc
    12181 agaccggagc cgcagacttt gttgatggtc atcgccgcga cgcgcgcatc acagccgccc
    12241 ttcagcgccg cttgccgcgc gggattctgt ccaagccccg cttgcaaaac gttgcccaga
    12301 atggcttcgt caatctgctt ggcgtcgagg cccgcgcggc gaatggcttc agcaaccgct
    12361 ttgccgccaa gttccggcgc tgagaatccg gagagaccac cttgaaattt tccaatcggc
    12421 gagcggacgg cggagagaat gacgggagtt tccgtactca tggtaagcct ccggaagagc
    12481 gacgcgcttc attataccgc gatgcaagga aggaggctgc ggcagcggga aaacggtggg
    12541 ggcggagaaa tccgccccct tcgaaacgcg atcctaattc gcggcggctt ctgcagcgcc
    12601 cggctggcct tcgtccgcgc tgggaccgta aagcccaggc acgggaattc cgagcaagcg
    12661 gaaatacacc gtgagctgcg cgcgatgatg gatgatgtgg ttgaagatca tgccgcggag
    12721 acacgccacg cgcggcatcg tcaaaatctc tttgccgccc gcgagcagct tccagggttt
    12781 catcatttcc tgatcgcttg cggtggagag agccgcgcgg gcttccgcga cgttcttgtc
    12841 gaacacggca agcagctcct tgcggttgtc cgttttcggc ggctggtagg ccgggccacc
    12901 aacgggggca tagtctaact ggtccgtcgt aagtgttacc gcggtccagc cgggcatcgt
    12961 cgcgatgtgg ccggcgagcc agccaaccgt gccggacttg tcatgcggtt tccagttcca
    13021 tttgtcgtcg ggacaccgtt cgagagtctt gcgcgtgttc tgcatctcct ggtcaaactc
    13081 accaagcatc atttgtccaa tggtcatgac ttcctccctt gcgaaattcg agattcgggc
    13141 gcactgattc taggcgaaca aaaagcgaat tgtcaagaac ttctttctcc cactccggct
    13201 ccccgtcttc cagttcgttg gctctgctct gcttagttgc gcgaacacga caattggtca
    13261 ctccggactt gcgtcaccgg agcggaactt cgccgagggg cttgacagcc tacgaagagg
    13321 gggcagaatg gcggtatggc gcaccaattt acgacctcct acttaggaga ttccatagat
    13381 gtttttcact attacaagaa actgggcgag cgggccatga cgcagtgccc ggaagaagcc
    13441 ctcttcacga ccctcgatgc cgaatccaat tcgatcgcca tcatcgtgaa acatatagcg
    13501 ggaaacatgc gctctcgttg gaccgatttt ctaaccaccg atggcgagaa gcctgaccgc
    13561 aaccgcgaca ccgaatttga agcgccgccg aaaacgcgcg cggaattgat ggaaatgtgg
    13621 gaacgcgggt ggaacggcgt cttcgacgct ttagaaccac tgagcgatgc ggatctaacc
    13681 cggacgataa ccatccgcac ggagccacac tccgtgatgc aagccgtcaa tcgccagctc
    13741 gcgcactatt cctatcatgt ggggcaaatt gtctatcttg cacggcactt cgctggtgac
    13801 aaatggcaaa cactcaccgt cccgaagaag aagtcggctg aactcaacgc gcgagtggct
    13861 gcaggcgaga tttcgcaacg ataggagcaa ctaaagttca caaacgatcg aagagattgc
    13921 cacccgattt gttgcatgct aggcttgatt cagtgagtaa accgatgatt agcccgagaa
    13981 tcagcgagct tgttttcgaa atcacggaag aacccgaagg tggcttttcg gcagaatgtc
    14041 tgaccgagag catattcact cagggcgaca catgggaaga actacgagca aacgtcaagg
    14101 aagcagtcga cgcttacttc ttcgacggtc cgaagcccca gtcaattcgc cttcgcttcg
    14161 tccgtgacga ggtcctttcc ctcggatgag actcccgcga gacttaagcg gccgcgaact
    14221 cgccgatggg ctttgccgtc attggaacta ccgggagatt caccaagtag ggagccatat
    14281 ttcgcttctc acggaatcac cggcccacca tagaattgca atccccgcgc acaagcacct
    14341 caagatcggc atgttgaacg gcatcctgcg cagcgtggct gtccacaaag gcgtcacccg
    14401 tcaagaaatc ctcgaatcaa ttctctaagc ttccactgtt cgtttcctct tcgcccagaa
    14461 ttccgatacc ctgtttacgt catggcgacc ttgtttgaga tggaaccgct cggtgaaagc
    14521 gggctgaagc tcaatgacgc gcagaagcga gcgatcaccc acggcgaagg accgctgctg
    14581 gtgattgccg gggcgggaac cggaaaaacg cgcgtcatca ccgagcgcat ccgccacctg
    14641 ctgcaatcgg acgagagcct ctccggcgaa aacatcctcg ggctgacgtt tacgaagaag
    14701 gccgccggcg agatgaaaac ccgcgtggtg aaggccacgg gcgagcgcgg caaggcggta
    14761 acgctggcaa cgttccattc cttctgcgaa acgctgctgg cggaggccga cccgcaacgg
    14821 gtaatgctgg acaagttcga tcactggatc ctgcttcggc ggaatctgca gcggctgcgt
    14881 ctggagaaat acaggagatt ggcggatccc gggcaattcc tgaacgattt cgtggagttc
    14941 ttttcgcgat gccaggacga actggtgtcc tgcgaagact atcagcacta tgctgccggg
    15001 ctggcggcgc gcctggaagc ggaacgggag gcgctggacg aagacaccct cgcggaacgc
    15061 ctggaaacgg tcgcgttaca acaggaactt gcgcgcgcct accgggccag cgaagaactg
    15121 ctgcgcgaga agaatcgggt ttccttcggc tcgctgatca ccggagcggt tgggctgttg
    15181 gagcaggatg cgcagctgcg ccagactctg caaaaaaaat accattacat cctcgtggat
    15241 gaatttcagg atacgaacat cgcgcagctt aggttgctgg agctcctggc cgcgcctaca
    15301 aggaacatcg tggtagtcgg cgacaacgac caagcgattt accgcttccg tggtgcttcg
    15361 ttcgggagtt tcaaactatt cttggagcgg tttgcgggat ggaacgaagg ccaggactcg
    15421 acaccgttcc gtgtggcact gacggagaac tatcgctcga caccgaacat tctgcgcgtg
    15481 gcgatgcaag tgatcggcca gaatgc
//
LOCUS       DASYVO010000421         5729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_168171, whole genome shotgun sequence.
ACCESSION   DASYVO010000421 DASYVO010000000
VERSION     DASYVO010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5729
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168171"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="VGR03_14135"
     CDS             complement(<1..1114)
                     /locus_tag="VGR03_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995466.1"
                     /translation="MKKLAALTLSLFLTVGTALADTPKDPDPQASKPAPAAKPKAAAK
                     KADKSAEISAQLEEVRKALQSQQEQIQQLKNELARRDQQITDARNAAAAADSKASEAA
                     AKATEAAASSAEAKTAATAVTSDVNDLKLGSDSLKGAVQDTQKKIIAAESPSAIHYKG
                     INITPGGFLDAATVFRNRATSSDINTPLTGIPYPGNALSKVTENNFTGRHSRLSLLVE
                     GKLANAKIGGYYEADFLGVGTTSNNRQSNSYVLRQRQVFAQVLFDSGLSFSGGQMWSL
                     AAENKKGISNRTEIPPLTIDPQYSVGFTWARQYGFRVVKSFNHDKFALGFSVEAPQTT
                     IGGRGFNSFSNTSAIGVVTAFQNFWLNAPGAGGGLYN"
     gene            complement(1501..3525)
                     /locus_tag="VGR03_14140"
     CDS             complement(1501..3525)
                     /locus_tag="VGR03_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995467.1"
                     /translation="MKKLAALTLPLFFTLGTALADTPKEATPQATKAPQTAKPKAAAK
                     KADKSAEIAAQLEELRGALQSQQEQIQQLRNDLAKRDQQIGEARDAAAAASVKAAEAS
                     SKAAEASNAAAQVSSVASALNSSVGEVKASNDALKAAVAEDQKAVKAVESPVAIRYKG
                     VTLTPGGYLAAETVFHSHALGADINTPFASIPYPGNSLAKVSEINFTGRASRTSLLFE
                     GKLEHVKLSGYYEADFLGVGTASNNRQSNSYVLRQRLAFAQAVFDSGWAVAGGQMWSL
                     ATENKKGLQNRQENLTMQVDPGYVVGFTWQRLYGLRVSKSWTDKFALGIAIEGPQTTF
                     GGRGFNSYSNTSATGVVTAFQNFWLNVPGASGGVYNAFDATGYSANKAPDLIFKAALD
                     PGWGHYELLGIVSTFRNRVYPCAVVGTNTLDTAPGTTTLNCAPDPTNPNPKPSALGAF
                     NDTRTGGGVGASFNVPLFSKKIDFGMKAVGGDGIGRFGAAQLADTTARPDGTLALIRG
                     EQALARIEFHATPKLDIYIYGGNEYAWRAAYTGYASVKITNTPAIPANGANPAYPATF
                     KTTISQTGIGGYGSPFANNTGCSTETSPTGTSAPGAGGTCAGDIRNIMDFTLGFWHKL
                     YQGPKGGVRWGIQYSYLTKSGWSGNNNTTTSVSPKAVDNMVWTSFRYYLP"
     gene            complement(4062..4865)
                     /gene="phoU"
                     /locus_tag="VGR03_14145"
     CDS             complement(4062..4865)
                     /gene="phoU"
                     /locus_tag="VGR03_14145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1995468.1"
                     /translation="MAATAGLTEVVLYDTLVNYLVSMARTVEGAMNRALDAIVSLESP
                     RTSALPSEVFLLEPRINEMEIVIDEHAIRLLRRGSFTDDEMRLIVATLKITNDLERIG
                     DLAVNLAERVVSLHEMQGAQVPEELAPMASAVRAMVSKSLGALIFRNVEMATQVLESD
                     DVVDQYRDHIFEQLLQHMTQQPTLVSPGLQFILATRHLERIADHATNIAEDIIFWVRG
                     LDVRHGRGLTVRPDQTDQTDSSSSEDSVITENSHVLHSHVTPACETERA"
     gene            complement(5024..>5729)
                     /locus_tag="VGR03_14150"
     CDS             complement(5024..>5729)
                     /locus_tag="VGR03_14150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1995469.1"
                     /translation="RDFVANVSHEFKTPLTAIQGFAETLLAGAIDDPQNRLRFLEIIL
                     EHSRRLARLTDDLLKLSKMDADRLELEIRRLSVSQFVDSCIETTQRPAAEKDLRISVN
                     LQQPLPDIAADRRRLAEVLQNLLDNAMQYTPAGGQIMVSALADGAEVTFTVSDTGIGI
                     PQADQPRIFERFYRVDVARSREVGGTGLGLSIAKHLVEVHGGRIWVESEVGQGSQFHF
                     TVPVFDPERAALRPAR"
BASE COUNT         1112 a         1546 c         1809 g         1262 t
ORIGIN      
        1 cgttgtaaag accgcctccc gcacccggcg cgttgagcca gaagttttgg aaagccgtga
       61 ccacgccaat cgccgaggtg ttcgagaaac tattgaagcc gcggccgcca atggttgtct
      121 gcggcgcttc cacagagaag cccaatgcaa atttgtcatg gttaaagctc ttcaccacgc
      181 ggaagccata ctgacgtgcc caggtaaagc cgaccgagta ttggggatca atcgtcagcg
      241 gcggtatttc cgtgcggttc gagatgccct tcttgttttc cgccgccaag ctccacatct
      301 gaccgcctga aaagctcaag ccactatcaa acagcacttg cgcgaacacc tggcgctggc
      361 ggaggacata actgttgctc tgccggttgt tcgaggtggt gccgacgcca aggaagtccg
      421 cttcgtaata cccgccgatc ttcgcgttcg ccaatttccc ttcgacgagg aggctcagcc
      481 ggctgtggcg tccggtgaaa ttgttctccg tcactttgct cagtgcattg cccggatagg
      541 gaatgccggt gagcggcgtg ttgatgtcgc tgctggtagc gcggttacgg aacaccgtgg
      601 ccgcatcgag aaagcctccc ggcgtaatgt tgatcccctt gtagtgaatc gcggacggcg
      661 attccgctgc gatgattttc ttttgggtgt cctggacggc gcccttcaga gaatcgctgc
      721 ccagctttag atcgtttaca tcggaggtca ccgccgtggc ggcggttttc gcctccgccg
      781 agctggctgc cgcctcggtg gccttcgcgg cggcttccga agccttggaa tccgcggcgg
      841 ccgcagcgtt gcgcgcgtct gtgatctgct gatcgcgccg ggcgagttca ttcttcaact
      901 gctgaatttg ttcttgctgc gactgcaaag ccttgcggac ctcttcgagt tgcgcggaaa
      961 tctccgcgga cttatcagcc ttcttggcag cggctttggg ctttgctgcc ggcgccggtt
     1021 tgctagcttg cggatcggga tctttcggtg tatcagcgag agctgttccg acagtgagga
     1081 acagcgacag cgtgagtgca gctagttttt tcatttcctc tctctttgga gcaaaattcg
     1141 atcgggtacc gaactttgtg cgtaccaccc cagcgccgaa ggtagaggct gcgttttacg
     1201 gctacgtcaa ctcaacatga atatcctgta acaagcagga aggattccgc gtaactcgtt
     1261 gacagggcaa tcgttgcaga atatttgggg cttcaagaac cgatgaaagt ggggaaggcc
     1321 attgttgaaa acaagcgcca cgttttgtgt ttctcaggat tgtaacaaag gcgaagcagc
     1381 ccggtccgtg cgctcagcga aaaaggatga agggcattgc gagacagaaa gggcagctcg
     1441 gtgaagagct gcccctgtcc gccacaaagt aggaatgtgc cgcagatggt cttgccggaa
     1501 ttacggcaag taatagcgga aggaggtcca aaccatgttg tcaacggcct tgggggagac
     1561 gctggtggtc gtattgttgt tgccggacca gccgctcttg gtcagatagg aatactgaat
     1621 gccccagcgg acgccaccct tcgggccctg gtagagctta tgccagaagc cgagggtgaa
     1681 atccataatg ttgcgaatgt cgccggcgca agtgccaccg gcgcccggcg cggatgttcc
     1741 cgtgggcgag gtttcggtcg aacaaccggt gttatttgcg aacggtgagc cataaccgcc
     1801 gattccggtc tggctgatgg tggtcttgaa ggttgccggg taggccgggt tggcgccgtt
     1861 ggccggaatg gccggcgtgt ttgtgatttt gacggatgcg tatccagtgt aggcggcgcg
     1921 ccaagcgtac tcgttgccac cgtagatgta gatgtcgagt ttgggtgtag cgtggaactc
     1981 gatccgggca agagcctgtt cgccgcggat caaggccagc gtgccgtcgg ggcgggcggt
     2041 tgtgtcggcc aactgcgctg caccgaagcg tccgatgcca tcgccgccga ccgccttcat
     2101 gccgaaatct atcttcttgg aaaagagcgg cacattaaag ctggcaccga cgccaccgcc
     2161 cgtgcgcgtg tcgttgaacg cacccaaggc actcggcttg gggttggggt tcgttgggtc
     2221 aggcgcgcaa ttgagtgtcg tcgtaccggg agccgtatcg agtgtgttcg tgcccaccac
     2281 ggcgcaagga tagaccctgt tacggaaggt gctcacgatg ccgagcagct cgtagtggcc
     2341 ccagccggga tccagcgccg ccttgaaaat gagatcgggc gccttgttgg cgctatagcc
     2401 cgtggcgtca aatgcgttgt aaacaccgcc gctcgcaccg ggtacgttga gccagaagtt
     2461 ttggaaggcg gtgaccacgc cggtcgccga ggtgttcgag taactgttga agccgcggcc
     2521 tccgaaggtt gtctgcggtc cttcgatagc gatccccaga gcgaatttat ccgtccagct
     2581 cttggatacg cgcaatccat agagccgctg ccaggtgaag cccaccacgt acccggggtc
     2641 aacttgcatc gtcagatttt cctggcggtt ctgcaatccc ttcttgttct cggttgcgag
     2701 gctccacatt tggccgccag ccaccgccca gccgctgtcg aaaacggcct gcgcgaaagc
     2761 caggcgctgg cgaaggacgt agctgttgct ctggcggttg ttggaagcgg tgcccacacc
     2821 gagaaagtcc gcttcgtagt agccactgag tttcacatgc tctaatttgc cttcaaagag
     2881 caagctggtg cgcgatgccc tgccggtgaa gttgatttcg gagactttgg ccagggagtt
     2941 tcccggatac gggatactcg cgaaaggggt gttgatgtca gcaccgagcg catgactgtg
     3001 aaagactgtc tccgccgcca gatatccgcc cggcgtgagc gtgacgccct tgtaacgtat
     3061 tgccacgggc gattcgaccg ctttgaccgc cttttgatcc tcagccacgg cggctttcag
     3121 ggcgtcgttg ctcgctttca cttcgccgac cgaagaattc agcgccgagg ccacggagga
     3181 tacttgcgcc gcggcgttgg acgcctcggc tgccttgctg ctggcttccg cggctttgac
     3241 gctggcagct gcggccgcgt cacgcgcctc gccaatttgt tgatcgcgtt tggcgaggtc
     3301 attcctcaac tgctgaatct gttcctgctg tgactgcagg gccccgcgca gctcttcaag
     3361 ttgcgcggca atttccgcgg acttgtctgc cttcttggcg gcagccttgg gctttgccgt
     3421 ctgcggcgcc tttgtggctt gaggagttgc ttctttcgga gtatcagcga gagctgttcc
     3481 gagcgtgaag aacagcggca gtgtgagtgc agctagtttt ttcattgcgc ctctctttga
     3541 aacaaaattc gaagatgtac agaaaaacat gtgtcccacc ccggagcaag aagtaaagtg
     3601 catcttacgg ttgcgtcaac caattgtata tatcccgtaa caatcggttt gctggaacgt
     3661 aaccctttga cggtcttgcg cttatggatt tgtcgtgctt caacaacagg tctaactgcg
     3721 aggaaaccca cagtcacaca actcatggcg cttcgtcaaa accgactgtt gttggagtga
     3781 gcgactcctc tctaaggtct ccaaggaaca gcctgcgcct tctgcacccg gaaggactta
     3841 tttcggcgct tccatcgtat gggcgacaac aatcggggtc aagtggcagc gatacgagtg
     3901 ctggctcatt tcgggagtta atttcgtgac gcagctctag gcgaatctca cagcgagtcc
     3961 gctgagctgg tttggatcgt gggattcgat actactgaag ggggagatga ggaccggagc
     4021 caggggtgca ccggtccccg aggtaggact ttacgtgaag atcaagcgcg ctcagtctcg
     4081 caggccgggg tcacgtgcga atgaaggacg tgtgaattct ccgtgatgac tgaatcttca
     4141 gaagaagacg aatcggtctg atcagtctgg tcggggcgga cagtaagacc acgaccgtga
     4201 cgcacatcga gaccacggac ccagaagatg atgtcttcgg caatgttggt ggcgtggtct
     4261 gcgatgcgtt cgaggtggcg cgtggcaaga atgaattgca ggcccgggct cacaagcgtg
     4321 ggctgttgtg tcatgtgctg gaggagctgc tcaaaaatgt gatcgcggta ttgatcgacg
     4381 acatcgtcac tttcgagaac ctgcgtggcc atctcgacgt tgcggaaaat gagcgcaccg
     4441 agactcttgc tgaccatggc gcgcacggcg gaggccatcg gggctagttc ttcgggaact
     4501 tgtgcgcctt gcatttcgtg caaagagacg acgcgctcgg cgagattcac ggcgaggtcg
     4561 ccgatgcgct cgaggtcatt ggtgattttc agagtggcga cgatgagccg catctcatcg
     4621 tccgtaaacg aacctcggcg gagcaggcgg atggcatgtt catcgatgac aatttccatt
     4681 tcgttgatgc gcggctcgag gagaaacact tcgctgggga gggccgacgt gcggggactt
     4741 tccaggctaa cgatggcgtc gagcgcgcgg ttcatggcgc cttcgacagt gcgcgccatg
     4801 gaaacgagat agttcacgag cgtgtcgtag agcacgactt ccgtgagtcc ggcagttgct
     4861 gccattggtc ccctttcgga gcggaggtcg ggaagtcaca ccctcgctgc taagcaaaat
     4921 tctgccttgg aaatggttac agcttgttca caataatgtg aaaatgcggg gaaagatggg
     4981 agttagaggc ggtaccggct gccgccggcg ccgcgccgtt tggtcagcgc gcggggcgca
     5041 gggcagcgcg ctccgggtcg aagacgggaa cggtaaaatg gaactgtgaa ccttgcccca
     5101 cttcgctctc gacccagatg cggccgccgt gaacttcgac gagatgtttg gcgatggaga
     5161 gccccagccc ggtgccgccg acttcgcggg aacgcgccac atcaacgcga tagaagcgct
     5221 cgaagatgcg gggctggtcc gcctgcggga tgccgatgcc ggtgtcagaa accgtgaagg
     5281 ttacttcggc gccgtccgct aaagcgctga ccatgatctg gccgccagcg ggagtgtatt
     5341 gcatcgcgtt gtccagcaaa ttttgcagaa cttcggcgag acggcggcgg tcggcggcga
     5401 tgtccggcag gggttgttgc agattcacgg aaatgcgcag atctttttcg gcggcggggc
     5461 gctgcgtggt ctcgatgcag ctatcgacaa attgggaaac gctgaggcgg cggatctcca
     5521 gttccagcct gtcggcatcc attttcgaaa gtttgagcag atcgtcggtg agccgggcga
     5581 gacgccgcga gtgctcgaga atgatctcga ggaagcggag gcgattttgc gggtcgtcga
     5641 tggcgccggc gagaagcgtt tccgcaaaac cctgaatcgc ggtgagcggc gttttgaatt
     5701 cgtgggaaac gttggcgacg aaatcccgg
//
LOCUS       DASYVO010000422         3061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_125609, whole genome shotgun sequence.
ACCESSION   DASYVO010000422 DASYVO010000000
VERSION     DASYVO010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125609"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VGR03_14155"
     CDS             complement(<1..524)
                     /locus_tag="VGR03_14155"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEV1995470.1"
                     /translation="MTAPNPLRETLSAGQFCYVVELVASRLTREARLLEVASKLAQVP
                     GVVAGSITSYAGGALGHDPVRVGTAARARGLTPNIHLTCVGRKRAAAWKTLEDLHALG
                     IENVLALTGDYPKTAGIQAPENLFDFDSVQLVHLINEARQRGMPFWISVAVSPFKYTE
                     ADCAYQYLKLEKKIA"
     gene            complement(526..1527)
                     /locus_tag="VGR03_14160"
     CDS             complement(526..1527)
                     /locus_tag="VGR03_14160"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995471.1"
                     /translation="MTLPILPTTVVGSYSMPGWLERLKTEYYARRISRRDLDEIHDTA
                     VKAAIKDQEVAGLDIITDGELRRDNMIDYFAERLPGVQIDHASKKFHYDFYDSKVLGK
                     LPMAPLGLVNDFKFLRAYTEREAKFCVTGPHSLSKRIRNEHYPSEEEFAMDLARVMNP
                     ELKAIAKAGARFIQIDEPNYSGFPEDLTWGVKALNALVEGVEAKIAVHICYGNRYGKP
                     SWEGSYRYLLPGILDAKVHQLTLEFARRGGEDLELFRELPARFELGVGVIDVKTQAIE
                     TPDLVADRIRKALAVIPAERIFVLPDCGCLHLPRDVAFAKLSAMVEGTRLVRKELKH"
     gene            complement(1533..2984)
                     /locus_tag="VGR03_14165"
     CDS             complement(1533..2984)
                     /locus_tag="VGR03_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995472.1"
                     /translation="MIVQLNHLQKKSAASTTQEVVDTFYSLLEEGHIEQEQFARLRVQ
                     LDWIQYRQNFRECVMATTATSDRPEQFPGLEVHIDTRQVTPGCLHEAILRTMGKAGDP
                     PSPDPDGVLLEDFRSFRESIAWGFNRLYWHRLADWEQFTGRGYEQALPGGKSDANHPA
                     AVADCVADFWTLLRDLENRKQLPEELFVMEIGVGMGTRSGLWLARFHALDRERGTNYY
                     PRLKFLLGDYSLATLERSRPAVKEHLELCSFVVLDALNPLKNLAFLRHKILHIHLTNV
                     YDNLPDEEFARRDGRLYAVQVRAYIPAADAQRISKDLEIPAADIPRTVNRLLEGGPDY
                     LSDRRRGVAFWQEVWKAMRLEERLVNLDDLPGVSIPGNLRASQLEDVLAQAPSDVRFH
                     LSSGALESFHNTLPLLHPRGFLQVQDIFVTNFNEYRLGFHGPGKLDGSIVNWVNGALL
                     REVGERAGYDVHFAPFHYRKGSKTSILYTTQRE"
BASE COUNT          649 a          894 c          885 g          633 t
ORIGIN      
        1 gcaatcttct tctccagctt tagatactgg taagcgcaat cggcctcggt gtacttgaac
       61 ggagatacgg caacggaaat ccagaacggc attccacgct ggcgcgcttc gttgatgagg
      121 tggacgagtt gcacggaatc aaaatcgaac agattctcgg gagcttggat accagcagtt
      181 ttgggatagt ccccggtcaa cgccagtaca ttctcgatgc cgagggcatg gagatcctcg
      241 agcgttttcc acgcagccgc gcgtttgcgg ccgacgcacg taagatgaat gttgggtgta
      301 agcccgcggg ctcgcgcagc ggttcccacg cgaacgggat cgtgacccag cgcgccacct
      361 gcataactcg taatgcttcc tgcaacaacg cctggcactt gcgccagttt cgaggcgacc
      421 tcaagcaggc gagcttcccg ggtcagacgg ctggccacca attccacgac gtagcagaat
      481 tggccggcgc tcaatgtttc ccgcaatggg ttcggtgcag tcacttcagt gtttcagctc
      541 cttgcggacc aagcgggtgc cttccaccat cgcggaaagc ttcgcaaaag ctacatcgcg
      601 aggcagatgc aagcatccgc aatccggcaa gacaaaaatc cgctcggccg gaattaccgc
      661 gagcgccttg cgaatgcggt cggcaaccaa atccggcgtc tcgatggctt gcgtctttac
      721 gtcgataacg ccaacgccca gttcgaatcg cgctggcagt tcccggaaaa gttccaggtc
      781 ttcgccaccg cgccgggcaa attccaaagt gagttggtgc actttcgcat ccagaattcc
      841 agggagcaaa taccgatagc tgccttccca ggaaggtttc ccgtagcgat ttccataaca
      901 aatgtgcacg gcgattttcg cttcgacacc ttcgaccagt gcgttcaggg ccttcacgcc
      961 ccaagtcaaa tcttcgggaa atcccgagta gttgggctcg tcaatttgga tgaagcgcgc
     1021 tccggctttt gcgatggcct tcagttcggg attcatcacg cgggcaaggt ccatggcaaa
     1081 ttcttcttcg gagggatagt gttcgttgcg gatgcgtttc gatagagagt ggggacccgt
     1141 gacgcagaat tttgcttccc gctcggtgta ggcgcgcaga aacttgaaat cattcacaag
     1201 gccaagcgga gccatgggga gcttgccgag caccttgcta tcgtagaaat cgtagtgaaa
     1261 tttcttggag gcatgatcga tttgcacgcc gggaagccgc tcggcgaagt aatcgatcat
     1321 attatcgcgg cgcaactctc cgtcggtgat gatgtcgagg cccgccactt cctggtcctt
     1381 gatggcggcc ttgacggcag tgtcatggat ttcgtcgagg tcgcggcggc tgatgcgccg
     1441 ggcgtagtat tcggtcttga gccgttcgag ccagcccggc atcgagtagc tgccaactac
     1501 ggtggtcgga agaatgggaa gagtcatcgg cattactccc gttgcgtggt gtagagaatc
     1561 gatgtcttcg aacccttccg ataatggaaa ggtgcgaagt gcacgtcgta cccggcgcgt
     1621 tccccaacct cccgaagcag cgcgccattt acccagttca cgatggagcc gtccagtttt
     1681 cccggtccat gaaaacccag ccggtattcg ttgaagttgg tgacaaaaat gtcctgcacc
     1741 tgcaggaagc cgcgcggatg aagcaatggc agcgtgttgt gaaagctttc gagcgcgccc
     1801 gagctcagat gaaagcgaac gtcgctggga gcctgcgcca aaacatcctc aagctgagaa
     1861 gcacgcaggt tgccggggat ggaaacaccg gggagatcgt ccagattgac gagccgctct
     1921 tcgaggcgca tcgctttcca gacttcttgc cagaaagcca cgccgcgccg gcgatccgag
     1981 aggtaatcgg gaccgccttc gagcagccgg ttcacggtgc gagggatgtc agccgcgggg
     2041 atttccagat ccttgctgat acgctgcgcg tccgccgcgg gaatgtaggc ccgcacctga
     2101 acggcgtaga ggcgtccatc cctgcgcgcg aattcctcgt ccggcaggtt gtcgtacacg
     2161 ttcgtcaaat gaatgtgaag aatcttgtgg cgcaggaacg cgagattctt caggggattg
     2221 agggcatcca gaaccacgaa gctgcatagc tcgagatgct ccttgaccgc gggacgggag
     2281 cgttccagcg ttgccagcga atagtcgccg agaagaaatt tcaggcgcgg atagtagttg
     2341 gttccgcgtt cccggtcgag cgcatgaaac cgcgccagcc agaggccgga acgagttccc
     2401 atgcccacac caatttccat gacaaaaagc tcttccggta actgcttgcg gttttccaga
     2461 tcccgaagca aggtccagaa atccgcgacg caatccgcca cggcggcagg atggttggcg
     2521 tccgacttcc caccgggcag ggcctgctcg tagcctctcc cggtgaattg ctcccaatcc
     2581 gccaagcgat gccagtagag ccggttgaat ccccaagcga tgctctcgcg aaacgaccgg
     2641 aagtcttcca ggagcactcc atcgggatca ggactcggcg gatcaccggc tttccccatc
     2701 gttctcagaa tcgcttcgtg caggcatccg ggcgtcacct ggcgggtatc gatgtgcact
     2761 tcgagccccg gaaactgctc tggccggtcg ctggtcgccg tggtagccat aacgcattca
     2821 cgaaaattct ggcggtactg aatccaatcc agctgcacgc gcagccgcgc aaactgctcc
     2881 tgctcgatgt gcccctcttc cagcagcgaa tagaaagtgt ccaccacttc ctgcgtggtg
     2941 ctggcggcag acttcttttg cagatggttc agttgcacaa tcatcgttca caaaccgcgc
     3001 gcacgcttgc acctcagcca ctgaaagaat ctttcgttag acgctcgccg cctgccgcgc
     3061 a
//
LOCUS       DASYVO010000423        31240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_249673, whole genome shotgun sequence.
ACCESSION   DASYVO010000423 DASYVO010000000
VERSION     DASYVO010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 31240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31240
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249673"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VGR03_14170"
     CDS             complement(<1..630)
                     /locus_tag="VGR03_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEV1995473.1"
                     /translation="MPTHATPKLRVFLVFGGVLASLVLGMSGSFAHHDPTTNPPTFYR
                     DVLPILEQHCQSCHRAGGIAPMPFETYEETRPFADAIRRATQEKSMPPWFADSKVGQF
                     SNDPSLSPAEIGTLAAWAEAKSPAGTTRDAPPSPRWAESWSIPKPDLVLKMPQAVPLP
                     ASGDIEYTFEIVPTNFKEDRWVQMAEVLPALRSNVHHAVVYVRPPDSNWL"
     gene            842..2773
                     /locus_tag="VGR03_14175"
     CDS             842..2773
                     /locus_tag="VGR03_14175"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV1995474.1"
                     /translation="MAGAKDKSKLGYRILLGTVVLVLGGSMLLYLVPQSPGTGSGEIS
                     TDTLAKIGDESVSAQDVRQQLNQIEQRNPNMKPLEALYAQQILKQLVFEKEIEYEAKR
                     LGIAVSDQERADRIRQYVPTAFVGGAFVGMDRYSAEVQKSFQLTVPVFEELIRQGLLE
                     DKFRKLVTDGISVGPAELQDEFRYKNEKVKLDYALIKPEDLEGKILPDEAEIRAAYEK
                     NKSKYQVPERRVARYALVDVNQIRQNVQVSDDMLKLQYQANIQQYQVPNRVHVEHILF
                     MTVSKTTDAEVEEIKKKAEDVLKQVKKGGKFEDLAKKYSEDPGSKGKGGDLSWITQGQ
                     TVPEFEKTAFSLSPGQVSDLVKTQYGFHIIKVLEKETAHTKPFDEVKDSLRAPLLLSQ
                     ADKLASDTADQLSAAIRQSNKISLDDLAKQYHLTVNETRPIGAADPLLELANSQEAKD
                     AIFRLKQDEVGLPVRTDRGYVVLSVKSIQRSHQGSLEEVRDPVLTDLKREKSTELAKS
                     KSEELARRVKAGEKFDAAARALGLELKTSDPIARDGSIPGAASGKQVGAAFNLKTGDL
                     AAPLSMGQNWLVYRVAEKTEANPADFGKQKEQLAKELLQSKRNLAFEAFQTALDTRLK
                     KEGKLKLMPEKLKSFGTFG"
     gene            2991..3416
                     /locus_tag="VGR03_14180"
     CDS             2991..3416
                     /locus_tag="VGR03_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995475.1"
                     /translation="MAARISAARNDYRCSIDRNQSGKYCVRIQVHYPRHAWTLGIFFL
                     ASSFDRAMKKIEEALDFLQRQEEKLWFWGVDRAEDMGFSAEFLKDAGLRLDRRTEFPR
                     KATNVSLTPEREVPAFVLGPMRRGLAESVELSRSAAAGD"
     gene            complement(3474..5669)
                     /locus_tag="VGR03_14185"
     CDS             complement(3474..5669)
                     /locus_tag="VGR03_14185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV1995476.1"
                     /translation="MRLPGKIFSIFTASGLLFQAAAPVLAQQTGQAGMNEARIHVTSE
                     LVLVNVVAHDKNGNLVRDLKKGDFTIFEDGKKQEISSFDFENVDELTTAGAAEATVSG
                     VAGGTLLRSGKQTAPSLDARDRRLILLFFDFSAMEPEQIDRSVEAAKKFVGTKMQPAD
                     LMALVSLATNMRVDLDFTDDKTKLLAALTSYNSGQGQGFENGSTGSTEGATETSGAFS
                     ADDTDFNTFNADRKLLALQSLMQALGKLPQKKSLIYFSNGISQSGADNQSALRATTAA
                     AVKANVSIYSLDIRGLQAFPPGGEAQAASLRGQSAYSGAAVLNDLNGNAASQDTLATL
                     SSDTGGKAFFDSNDFGSVFSQVQKDSSAHYVLGFTSNNALKDGKFRRLKVQVNRSDLK
                     LDFRAGYYAGRDFEHLNRADREQQLEDELDAQLPRVDVPLYAGAAYFRQDDSHYYLAV
                     SLVIPGSQIPFVTEKEKDNATIDIMGEALGGGKLRVGQLRDTVKLAVESTQQVRRKNV
                     QYNTGFVLAPGSYHLKFVIRENQTGRMGSFETDVQIPDLRKSPLKMSSVVLSSLRAPV
                     TSKKNGANPLVRDQMELVPNIKHVFAPDQHLYLQYEVYDAAKGKNPAPAAIDAQKGDQ
                     KGGQNGTAKDAPAAKPPKDSIRVFTSIEFLQGATKIYESKAVVANEFTAPDRKAVIFQ
                     IDLPLQALKPGLYLCQVNVIDDVVGSFSFPRFPLLIREAVPAAPSKAAD"
     gene            complement(5681..9076)
                     /locus_tag="VGR03_14190"
     CDS             complement(5681..9076)
                     /locus_tag="VGR03_14190"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV1995477.1"
                     /translation="MKSIARGILILHFVAILFASSSRAQEAAPASPPPTAQTATTPAA
                     VPAYEINGSARSGKTPLPGVTVTAANTLTGKKYAAATNSEGKFGLSGMTRGRYVVRVE
                     FMGFAAFTQEVVLNPENPSAKVDAELILASRQQEQSNSNNAAMAAAGRGFQSLATDSA
                     LSSLAGGSSGFGGAGGGSQGNSDLSSLPMNGAGAEGPTESVSISGAQGRTQDVGGGSE
                     QDLQERIQEFRDRMQREGGAGFGGGGGGGFGGPGGALIALGRMPRGFNINQPHGVLYF
                     SDNNAGLDARSYSLTGIASPKANYNQSRFGVNIGGPLNIPKIFNGGNKWFFFGGWNGS
                     RGSSPYDAFSTVPTNDERGGNFSAATYNDGKLVQIFNPKTGQPYQFTGVQNEIDPSLI
                     SPAATALLKYIPLPNIATTASGQNFHYVTSAESNSDSVILRLIHNFGASSGPGFGPFG
                     GGSGGGGSGGGRRRSQNNINFGLNWSRSSSSIVNPFPSLAGGTGTQGLNASAGWTYGK
                     GRVTNIFRVNYNHNHISTTNLYSNVTDVAGPGGAGIGGISHDPFDWGPPGISFTSFGG
                     LSDPTPRRELDQTYSFSETVSWNRGKHNWHFGGDYRRILQSFRSARNAEGSFVFTGFA
                     TSQYTTGSTQPVTDTGYDFADFLLGFPQQTSLQSGTNSYNFRANAFDFFAQDDWRFRS
                     NLSFNLGLRYEYNGPYTEANERIANLDVAPGFTSAAVVLPGHAGPFNGIFPASLVRPH
                     RNNFAPRIGMAWKPQKQTVVRAGYGINYNLAQYGIMIQNFAFQPPFANTATNTTDVNG
                     LIGTNPLTLTTGFPAAGNTVTNNFGVNPNYRLGYVQIWNLDVQRELPGGVVLNAGYNG
                     AKGTRLDTERALVVSGGQPFIYESSEGNSILHAASVRVRKRMTKRLGLSAQYVFSKSI
                     DDASSIGGGGSVVAQDPFDISADRGLSSFDQRHKFTGNWIYDLPVGDNRRFVTKGEWS
                     HILGGWQWSGDFSIGSGLYFTPRVLGGGLDIGRGVSGSLRANAVAGQSISISDPTALK
                     WFNTAAFCAPSVACVNPNGSSFGNAGRNTIEGPGQVSMNMSLNKTITIKESRALDLRI
                     SANNVFNTVRFTSINTVVNSFTFGEVTGTGGMRRVTIQARFRF"
     gene            complement(9098..9865)
                     /locus_tag="VGR03_14195"
     CDS             complement(9098..9865)
                     /locus_tag="VGR03_14195"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEV1995478.1"
                     /translation="MMTHGRQFLRLSLLFLVAATAGRAQTPAPAALGQDQGATPTARL
                     TQRPEDGRGLPLLGKITAIKKESLELAKPDGSTATVKFTDKTEYRKDRQGAKREDFKV
                     GDFVLVRGKENADHSVTAQSIGGRSGGGAGGGGQGFGEMGKDIVVGEVKSVDAPKLTV
                     LRPDNVTQTLELNEETSLRRGRESITMADIQPGDHVVVRGAVENNAFVPKNVMILSPE
                     QWKRLQEMGMINGKGPAGNAPAGSKPATAPKSKPPQL"
     gene            10220..10681
                     /locus_tag="VGR03_14200"
     CDS             10220..10681
                     /locus_tag="VGR03_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995479.1"
                     /translation="MNSLKSLNTPLLAVLLLIAGNATARAQTTQQTGDQGQSGTTTPK
                     KKTAADAAASKSPGAPASASSATPPASTAPAGKSAAAAPASTPGNSAPASKPDTTQKT
                     PPAKSAAMVWVNTDSGVYHKPGTRWYGKTKQGKYMTEADAIKAGYHAAKKQ"
     gene            complement(10785..11060)
                     /locus_tag="VGR03_14205"
     CDS             complement(10785..11060)
                     /locus_tag="VGR03_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995480.1"
                     /translation="MAERPVLVGLIPQVVVIDEGDQVSWISDAGNLRVEFDVNRCPFS
                     SNVFQAPAGMRLLSGPPRPGSKAATYKYRLWLNDQLVGHGEVILREK"
     gene            11398..12255
                     /locus_tag="VGR03_14210"
     CDS             11398..12255
                     /locus_tag="VGR03_14210"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEV1995481.1"
                     /translation="MASASRYLSIPLPNGSRISVLLSLPFVLVHLASLLIFFVPFHWY
                     YLVACLGLLAVRMFFVTAGYHRYFSHRSFKTSRVFQFVIAFMATSSSQKGVLWWAAHH
                     RHHHRHSDQELDLHSPTLFGFFWSHVGWIISDKYNDTRLEYIGDFAKFPELRWLNKYH
                     LVPPVTLAVVIWLIGGWPLFIWGFCLSTVLLWHDTFTINSLSHLFGSRRYETTDTSRN
                     NWLLALLTLGEGWHNNHHHFMASARQGFFWWEIDITYYTLKVLSWFGIVWDLRKVPTH
                     LLTQDRLAA"
     gene            13006..14076
                     /locus_tag="VGR03_14215"
     CDS             13006..14076
                     /locus_tag="VGR03_14215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV1995482.1"
                     /translation="MPRRLLVVMLAACSILVQAQQSSTQETSPGQTQAQLPAAQQSEA
                     RTKISVNSELVVLPVTVKDRYGNLVPDLQREEFRIFDDNVEQSIEVFTAEAIPLSLVV
                     LVDDDLKSKDAAQMAPALRAITAGINTSDEVLICRFDLSFYPGDGFTGDLDKLRADLK
                     DAQDHSGPSTAGPVPFVTPPSSHARGVGEPPPGPATNLGSRPTKALDDAVHSAAGLLH
                     DRGASRRKIILLISDGLNGPQFNRHTYADTLEALLHDNISVYSLAVGHSLKRKFSRLV
                     NYASDSGGDIYYAVQSAALERLYSRITEEARHEYTLAYVPRGNSRNANYHKVEVRTTR
                     GAVTIKTRRGYYTGPLVDLPKQ"
     gene            14297..15511
                     /locus_tag="VGR03_14220"
     CDS             14297..15511
                     /locus_tag="VGR03_14220"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEV1995483.1"
                     /translation="MAQSTHYSVKLVADFERQLLRGDETIEFQADAGVVEWQKQAGLR
                     VVSADVTDGEVTVAEQAVRVRLPSGGRHSLKLKYTAAAGRGIKWFAGNAGLATAFYCE
                     AWMVCDNSPGQRATLQLKIAIPFSPLTHGGSGFRVVGPGRQGKEWRGRDGDHFVFEQS
                     DPVQTYLFSFGVARLAVAIDGMFSIYAQNMDANKAAFAKTADAYAFLRAAASVDPMNA
                     HYTQVFLPGPSMGQEAAGIALMSQNDLNDLTGKDDVQLVAHELAHQWWGVTLGIRSWS
                     DFWLNEGFAELMSDAYIEKHQGRAAYDQQIAELQDRMKKLREEGKDRPLHWEKWKDAH
                     EALGQIPYVKGALFLARLRTELGDEIFWRGIGLYTSGSVRRLVDSGDFERAMEKASGR
                     DLKALFDEAVYH"
     gene            15963..16850
                     /locus_tag="VGR03_14225"
     CDS             15963..16850
                     /locus_tag="VGR03_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995484.1"
                     /translation="MAFYKGVITEWYPRASRVEPAANIFDATLYQQHADGSIAWDSVT
                     LSPTLRTEFPRENPDNHYYAARMTSSTPLRVKTPAGEQQEKFLFYRGVSTFPVPLSAK
                     MTAAGKLLIENRSNEEFPNTILFERRGEKVGYRLGGALQKEMNLDAPELTASIENLGR
                     DLEGMLVAQGLYQDEAHAMVETWSGSWFEEGSRLLYIVPTAFVAGVLPLSINPAPAQT
                     VRVFVGRLEIVSPATEKAVERALFTGDSATLKMFGRFLEPILETMIQKESNPARVQQF
                     YQALSFYYSSEIAQNIRRD"
     gene            complement(16921..17361)
                     /locus_tag="VGR03_14230"
     CDS             complement(16921..17361)
                     /locus_tag="VGR03_14230"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1995485.1"
                     /translation="MLRPLVFLLALSSFPFAQDISRAQGDETKIIALENLWNQMQIKH
                     DADAMGKMLDSDFVLTDYDGTVMSKTQFLESIRDTSNQLTVEVSTDMKLHRHGDTVIV
                     IGATREKGTEKGKPYAHQGRFTDTWIKKDGQWLCIASQLTLIGK"
     gene            17628..18836
                     /locus_tag="VGR03_14235"
     CDS             17628..18836
                     /locus_tag="VGR03_14235"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEV1995486.1"
                     /translation="MTQHVGELNPPVRLMLTPGPSSIDPRVYRAMATPLVGHMDPWFK
                     GCMEETQILMRQIFQTENRITMPLSASGSGGIEASVLNSLEEGDEGIVAVNGVFSERM
                     YEIATRASSKVVKVEAPYGKPVDAEDVRRAGQGKKIKIIGFAHGETSTGVVTGIDAYR
                     KVADELGALLIVDTVASLAAVPLDVDKQRIDICFSGSQKAISAPPGMSPITVSPTAEE
                     VFRKRKTKVQSWYFDLTTAMNYWGKDRLYHHTPPISLIFALREAMRLVMEEGLEARWE
                     RHRVNQLALIAGIEAMGMDLLVKNPADRLPTVTAVMIPGGVDDAKVRNQLLEEFNIEI
                     AGGFGPVKGKIWRVGLMGYCSQKPFVLLFLAALEKCLLDQGFRVPSGAGVGAAIRSYS
                     QVETAAAVSR"
     gene            18879..19253
                     /locus_tag="VGR03_14240"
     CDS             18879..19253
                     /locus_tag="VGR03_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995487.1"
                     /translation="MTTRPQLANSGAMSQFIGAFEVTSKLSGQTYQCRFSHMWNGIAT
                     RHADTIDTKFFIDGEAHVVGLAHTAFVKFREKAGRDLTDREASFVAAEYLRERLEEED
                     VRPIYDVPEAEVLRLIAQVGIK"
     gene            complement(19312..20556)
                     /locus_tag="VGR03_14245"
     CDS             complement(19312..20556)
                     /locus_tag="VGR03_14245"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1995488.1"
                     /translation="MSTKSIPLTSYQIRLLAVLALINFVNFAARQAIIPLIPLLRDHL
                     HATDAQLGSLQASLLVVLAAGSIPFGFLADRFSRKSIIALGIFFWSVASFAGGLVSSF
                     LFFFIARGMVGLGEAAYAPAAQSMISGAFPAERRAFAQAIFASGMLLGGATGQAFGGI
                     IGPRYGWQYVLFLVALAGILPGLALFGLEEPPRGPRSEVVPIARLLSVPAFVAMIAAG
                     ICITFSTVSLLTWGVDFAKTYKDFTLREASVLLALIALVASVFGVLIGGIVADRLHRR
                     LAYGRIMAIAVAFLLAAPFLLLAIQADEKATVLFGLSVAVFFMSWYHGPVTAVLHDMM
                     PRRAHATSVGVYMFATQLIGGLGPLVVGRISDLHDMQVALQIAVAVMVCGALLMLLVI
                     HYIRRDGLRHPTLAAFHAEPGD"
     gene            20641..21723
                     /locus_tag="VGR03_14250"
     CDS             20641..21723
                     /locus_tag="VGR03_14250"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1995489.1"
                     /translation="MMNKNTAPSANATTSDRLTTVLSYGVLLLLIYLVFRIYEPFLAA
                     LGWAAILVIFFYPMHHRLLRRFSASKAAVLSTLVVTFLLIVPAILVTTLFIREAFSVS
                     RGVEHTLQVQEAPLLAKSWAWIAHHVPGLDPNADVLEMLSQGVQKEAGFLAERLGTIL
                     RNIAAFIFDLFVMIFAMFYFFRDADQIIRGVRSILPFDAEHRETLMTQTRDLISASVT
                     TSLLLAALQGVIGGLGFALVKLPASLFWGVAMAFFSLVPVVGSGLIFVPAALWLGFTG
                     HWGRAILLLVICAGVSAIVDNVLRPFLLGGRTELSGLVIFISVVGGVGLFGMLGLVLG
                     PILVATAAGVLAVYMERPGNPPITAR"
     gene            21791..24241
                     /locus_tag="VGR03_14255"
     CDS             21791..24241
                     /locus_tag="VGR03_14255"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1995490.1"
                     /translation="MTTKPAPKPTELPAAKLRWRCELSRIPFETTAQAELREGFIGQE
                     RALRALKMGAELSAPGYNVFVCGLAGTSRGGTIARMIEELHPPTKESLDRCYVNNFKL
                     TDRPRLLALPRGQANAFKKDVQAGIDFLRRRIPQVFEGEPFQRQKGRIVERYTVREKE
                     LMDDFTRRIAREQFALGHMQVGAVALPEIFPVLEGQMVPIEDISKMVHEGKLESPVAE
                     DIERKYEQFRQEFTVVYRKTLTLSRELASELSYLEQEAASVLVDGVIEELKEKYAGPN
                     IAEYLEEVRHHLLDNLDPFKEHEGEGEHDEETPDGLPKAPGSPERDPFRVYGVNVILA
                     HSDEDKSPVIFETTPTYANLFGTIQRAYDTRGGWSSDFMDLRAGSLLRADGGFLIMYS
                     LEALSEVGVWRALKRTLNHNRLEIQPLEMFYPFGGSAQKPEAIDINVKVILIGDRSLY
                     ELLYEYEEDFRKIFKVRVEFDEEMAMSDGVIAEYAGRLRALSEKENLYPFDRGAFAAM
                     LEYGVRQAGRRNKVTARFIDIADLAREAHYNAAAAGESVVRAAHVRGALSSKMERHNL
                     IETRIREMIQEGTLLVDVEGSRVGQVNGLSVLEIGGYSFGKPVRITATAALGKTGLIN
                     IEREANLSGRFHDKGMHIIAGYLRSQFAQDKPLSLAASICFEQSYSGVDGDSASSTEI
                     YALASALSGLPLRQDIAVTGSINQQGDIQAIGGVNEKIEGFFDVCRIKGLTGTQGVMM
                     PESNVEDLMLREDVLEAVAAGKFHVWPVGKVEQGIEILTGMAAGKKNGDGKFEPGTVL
                     AMIDARLSEMAKTLKEFE"
     gene            complement(24357..26318)
                     /locus_tag="VGR03_14260"
     CDS             complement(24357..26318)
                     /locus_tag="VGR03_14260"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1995491.1"
                     /translation="MTETTQSALPKRFGAFEFDSRARQLRKHGHTIRLHGQPLEILGL
                     LLERPAEVVLREELRARLWPEDTFVDFEHSLNAAVNKLREALDDDANSPRFIETVPRR
                     GYRFIAPVERALPASAPDSTNQDAPAANQQATDLRVEKPAAEVPAPAGTRTQWRTVWL
                     AFIACAFLVALFIGLYGGGLRQRFLGRPDLGAIQSLAVLPLENLSRDPDQEYFADGMT
                     EALTTELAQISALKVISNTSVVQYKGTKKSLPQIAQELGVDAVVEGAVQRSGDKVEIT
                     VQLIHAPTDRHLLAKSYERGLRDVLALQREVAHAIADEIKAKLTPQEKVRLASTRTIN
                     SEAYEDYLQGRFLLSTQRERDVRKGIAYFQQAIQKDQNYALAYAGLAESYISLGQPWV
                     GGLSPKEALLPAKAAATKALEIDDSLGEAHLALARVIQLYDWDWPAVEKEYRRALELN
                     PNDAMAHNWYGEYLQEMGRNEGSFVQMRLAILLDPLSSGSESQLGDIFYTARQYDQAI
                     GAFQKALELEPDYTDAHVGLGWIYDEKKMYKEAIAELEKAVNLGNRHELIVASLGKVL
                     GDSGRKQEARKLLEELEERSKHRYVSPCLIALVQVGLGEKDQAIASLEQGYTDRDQWI
                     LYLKVDPHMDDLRSDPRFQDLLRRVGFPQ"
     gene            26594..29389
                     /locus_tag="VGR03_14265"
     CDS             26594..29389
                     /locus_tag="VGR03_14265"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEV1995492.1"
                     /translation="MNTTLRFLVTALAVLLSLGAASALADTRATPKPEPEVSFSTPRL
                     HFSYQLVGTSSPQMSEVVTNSGTAPLVITAMHVTGEDAADFAISSNFPLPVTVLPGQS
                     VTVNVSFAPGEPWTPGTRRARLKFTDNDGQQFVTLTGMGVNCGGPVPAVFSNGICADT
                     DLDGLNDVWEDNDYIDMNNNGRYDPGVDFKFPHKAPFVFSSVTKAGSGSGRMFPTVTD
                     PGVEVRPSTVVVTVAAGGEVGAATFTYSVSANEEGSGSGDDASTGGPLEIRPVVNIGG
                     NLRLLFYRSSPTESFVAGDTFSFTVGMGEETKVVDKNVPNIFVQYDYMDYDVPGNACV
                     ADPDCDQGGFQLNDVCHSGFCTHNHFPGDPLFRKVVDQFAAHGITLYIHPSPKAVPHA
                     AVVTWAKSTDQGNGPHTSCAGDEVPAVGITGSQAINFHNIKNRPGSDFALDPSRKMIY
                     HYAVLSHASICLTDINGPVGYCGACSQDRSTPSGFPRAGSSGNSELPGNDFIVSLGPS
                     LNNALGSVPSSPFIEEGIFMHELGHNLGLHHLGDVPAPERAPNYLSIMNYKYSFTGIQ
                     HAATPGSNVPVEDLRELDYSEHTLNTLVEGSLDETAGTSPLSSGYTGIVRFFNGVGGN
                     ARGAEAGPIDWDGINGIDPNPVSVDINKLNGTTETMVGYRDWDHTTGQSGSGGGACTT
                     SADCRVNAIRQLYWANVDRSIDTHEPCLNGRCQSLWLNFQNTAWGIADGLSTGSSRTI
                     FEPGAPGASAAPKINVSYDKTLLRGAPDAIITIVEFTDFRCAFCRQAESTLKEVLAKY
                     PGKVALAHKDYPLGGMDSQSALASRAARCAGEQGKFWEYRDLLLGSPKLDGEVLASSS
                     QTLNLNVKEFDSCLAAERYKAAIEQDLNDGNTAGVSATPAFFINGVRVTGAQPASTFE
                     KIIDQELGRLTQNSPGY"
     gene            29623..30918
                     /locus_tag="VGR03_14270"
     CDS             29623..30918
                     /locus_tag="VGR03_14270"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEV1995493.1"
                     /translation="MKAILRFLVTALAMILFARTGLQAQNVVIDWNNIASTTIITNAK
                     ESSTASGVWFAYVQLAVYDAVNSIDHRHHPYLFSTDAPDGASKDAAAVAAAHRVLANY
                     FPTQQASLDAQFASSLAAISDTPANISAGTAVGEQAALALIAARSHDGLLANVPYAPP
                     VGPGFWQPTPPKFAAPLTPWLGQMVPFTMTSAMQFFPDEGPDALNSEEWIDDYNQVKT
                     LGALNSAVRTPQQTEIGLFWTEHTGQQYARAFRNLATLKELNTSDTARLMAMLWTGYA
                     DSVIGCWNAKFSFSFWRPVTAIRAAGGNPQLTGDPAWTPLAATPAHPEYPAAHGCVTG
                     AVSTILAGYFGTPKVQFSVDSLVTHTTHTYDSTNDLMEEVEAARIYAGFHYHHSVVEG
                     RELGRKVAHQLMKEFFRPSHEDREENAEIGRDTNAEHRN"
BASE COUNT         6619 a         9086 c         9000 g         6535 t
ORIGIN      
        1 cagccagttc gaatccggcg ggcgaacata cacaacggcg tgatgcacgt tcgaacgcag
       61 cgccggcaga acttccgcca tctgcaccca gcgatcctcc ttgaaattcg taggaacaat
      121 ttcaaaggtg tactcgatgt cgccgctcgc aggaagcggc acagcctgcg gcatcttcaa
      181 gaccaaatct ggcttgggga tactccagct ttccgcccag cgcggtgacg gcggcgcatc
      241 ccttgttgtc ccagccggcg atttcgcttc agcccacgcg gcaagcgtgc cgatctccgc
      301 cggactcagc gatggatcgt tggaaaactg tcctaccttc gaatcggcga accacggcgg
      361 catggatttt tcctgcgtcg cgcggcggat cgcgtcggcg aatggccggg tctcttcgta
      421 cgtttcgaac ggcatcggcg cgatgccccc ggcgcggtgg caactttggc agtgctgctc
      481 caggatgggc agcacgtcgc gataaaatgt cggtggattc gttgtgggat cgtggtgcgc
      541 gaacgatccg ctcatcccca acacaagcga cgcgaggacg ccaccgaaaa caaggaacac
      601 acgaagtttt ggagtggcgt gagtcggcat gcggttacga tgcgcggcct ccgtctgcgg
      661 tggctcgctt ctgccagtct actatttcac atcttgcatc gttggaccga gccgggcgaa
      721 tgcccattcg ctgattctgg agaagtcccc ggacctggcg ggggcggacg attgtgtttg
      781 tgacccgtgc ctatatctcc tataatccta agtttgtcta ctgacgtggg agattcatcg
      841 gatggctggc gcaaaagata aaagcaaact tgggtaccgc atccttctgg ggaccgtcgt
      901 cctggtcctg ggaggcagca tgctgctcta cctcgttcct cagtcgccgg gcacgggcag
      961 cggcgagatc tccacagaca ccctggccaa gatcggcgat gaatcggtta gcgcgcaaga
     1021 cgtgcgccag cagctcaacc agatcgaaca gcgcaacccc aacatgaagc ccctcgaggc
     1081 cctgtacgct cagcagattc tgaagcagct ggtgtttgaa aaagagattg aatacgaggc
     1141 caagcgcctc ggaattgccg tgtccgatca ggagcgggcc gatcgcattc gccaatacgt
     1201 tccgacggcg ttcgttggcg gcgcgtttgt cggcatggac cgctattcgg ccgaagtcca
     1261 gaagagcttc caactgaccg tgcccgtgtt cgaagaactc atccgtcagg gcttgctcga
     1321 ggataaattc cgcaagctcg tgaccgatgg gatcagtgtg ggacccgccg agcttcagga
     1381 tgaattccgc tacaagaatg aaaaggtgaa gctggactac gcactaatca agccggaaga
     1441 cttggaaggc aagattttgc ccgatgaagc ggaaatccgc gcggcctacg aaaagaacaa
     1501 gtcgaaatac caggtgcccg aacggcgcgt ggcgcgctac gcactcgtgg acgtaaacca
     1561 gatccgccaa aacgtgcaag tctccgatga catgctcaag ctgcaatatc aggccaacat
     1621 tcagcagtat caagtgccaa accgcgtgca cgtcgagcac attctcttca tgaccgtttc
     1681 aaagacgacc gatgcggaag tcgaggaaat caagaagaag gccgaggacg ttctaaaaca
     1741 ggtcaaaaaa ggcggaaagt tcgaagacct ggcgaagaag tactccgagg acccaggatc
     1801 gaagggcaag ggcggtgact tgagttggat cactcaggga cagaccgttc cggagttcga
     1861 gaagacggcc ttcagcctca gcccgggcca ggtttcggac ctcgtcaaga cgcagtacgg
     1921 gttccacatt atcaaggtgc tggaaaaaga gaccgctcac accaagccgt tcgatgaagt
     1981 gaaagattcg ctgcgcgcgc ccctcttgct ctcgcaggcg gataagctgg ccagcgacac
     2041 ggcggaccag ttgtccgcgg ccatccgcca gtccaacaaa atttctctgg acgatctggc
     2101 gaaacagtat cacctgacgg tgaatgaaac gcggcccatc ggcgcggcag accccttgtt
     2161 ggagcttgcc aattcccaag aagccaagga tgccatcttt cgcctgaagc aggacgaagt
     2221 aggcctcccg gttcggaccg atcgcgggta tgtcgttctt tcagtgaagt cgatccagcg
     2281 gtcacaccag ggttcgctcg aagaggtgcg cgaccctgtg ctcaccgatc taaaacgcga
     2341 gaagtccacc gagcttgcaa agagcaagtc ggaagaacta gctaggcgcg tcaaggcggg
     2401 tgagaaattc gatgccgccg cccgagccct cggtttggag ctgaaaacga gtgatcccat
     2461 cgcgcgcgat gggtctatac ccggcgcggc cagcggcaag caggtcggcg cagctttcaa
     2521 tctgaaaact ggcgacttgg ctgcacccct gagcatggga cagaattggc tggtctaccg
     2581 cgtggctgag aaaacggaag ccaacccggc cgattttggc aaacagaagg agcagttggc
     2641 caaagagctc ctccaatcca agcgaaacct ggcgtttgag gccttccaga cggccttgga
     2701 cacccgcctg aaaaaggaag gcaagctcaa gctcatgccc gaaaaactga agagcttcgg
     2761 caccttcggc tagttcaagt aacacaaact aaaagagtta tatatttgga acggcacgct
     2821 tgtctttcct tgcgcgtccc gataccctat tggtgtcttc cttgaaaccg aattcgaccc
     2881 gcaacgccgt tgtttggcga agctcgggtt ccggggccag agcccagaat tcggagcaga
     2941 agcaatcatg gccacctagc gccaggactc caaagatcgg tgatatcaac atggcagccc
     3001 gcatttccgc cgctcgcaac gactaccgat gcagcatcga ccgcaaccag tcggggaaat
     3061 actgtgtccg catccaggtg cactaccccc ggcatgcgtg gacgctgggc atcttcttcc
     3121 tggcctcatc cttcgaccgc gcgatgaaaa agatcgaaga ggctctggat ttcctccagc
     3181 gtcaggaaga aaagctgtgg ttctggggtg tggaccgtgc cgaggacatg ggtttttcgg
     3241 ctgagtttct gaaagacgca gggttgcggc tggaccgccg cacggaattc ccgcgaaagg
     3301 ctaccaacgt ctccttgact ccggagcgcg aagtgcctgc cttcgtcctg ggaccgatgc
     3361 gccgcggcct ggccgaatcc gtcgaactgt ctcggagcgc cgccgccggc gactgatcct
     3421 aagccgctta ttttgaaccg tttctctgcc gcagcactct tgcctgatag gtttcaatcc
     3481 gcggctttgc tcggtgccgc tggcactgct tcgcggataa gcaggggaaa ccgcgggaag
     3541 gaaaagcttc ccaccacatc gtcaatcacg ttcacctggc acaggtacag ccccggcttc
     3601 aaagcctgca gcggcaaatc aatctggaaa atgaccgcct tgcggtctgg agccgtaaat
     3661 tcgttcgcta ccaccgcttt ggattcgtaa atctttgtcg ccccctgcaa gaactcgatg
     3721 ctggtaaaca ctcggatgct gtcctttggt ggcttcgccg ccggcgcatc cttagctgtc
     3781 ccgttctggc cccccttctg gtcccctttc tgggcatcga tcgccgcggg cgccggattc
     3841 tttccctttg ccgcatcgta cacttcgtac tgcaaataga gatgctggtc gggcgcgaaa
     3901 acatgcttga tgttcggaac cagttccatc tggtcccgga ccagtggatt cgcaccgttt
     3961 ttcttgcttg tgacgggagc gcgcaggctg ctcagcacca ccgagctcat tttcagcggc
     4021 gacttccgga ggtcggggat ttgcacgtcc gtctcgaaag aacccatccg tcctgtctga
     4081 ttctcgcgga taacaaattt cagatggtag ctgcccggag cgagcacaaa cccggtattg
     4141 tactgtacgt tcttgcggcg gacctgctgc gtgctctcga cggccagctt cacggtgtcg
     4201 cgcaattgcc cgacacgcaa cttcccgccc ccgagagctt cgcccatgat gtcgatcgtc
     4261 gcgttatctt tctctttttc cgtcacaaac ggtatctgcg aaccaggaat caccagcgac
     4321 accgcaagat aataatgaga atcatcctgg cggaaatacg ccgctcccgc gtaaagcggc
     4381 acgtcgaccc gcggcagttg cgcgtccaac tcatcttcga gctgctgctc ccggtcggcg
     4441 cggttcaagt gttcgaaatc tctccccgca taatatcccg cgcgaaagtc gagcttcagg
     4501 tcggaccggt tgacctgcac tttcagtcgg cggaattttc cgtccttcaa cgcattattg
     4561 ctcgtgaagc ctaagacgta gtgcgccgaa ctgtccttct gaacctggga aaatacgctt
     4621 ccgaaatcat tcgaatcgaa aaaggccttg ccgccggtgt ccgaggaaag cgtcgcgagc
     4681 gtatcctgcg aagcggcgtt cccatttaga tcgttcaaca cggcggcgcc cgaataggcc
     4741 gattgtccgc gcaagcttgc cgcttgcgcc tctcctcccg gcgggaacgc ctgcaggccg
     4801 cggatatcca gcgaataaat cgatacgttt gctttcaccg ccgccgcggt cgtggcccgc
     4861 agcgccgact ggttgtccgc acccgactgg ctgattccat tgctgaaata aatgaggctc
     4921 tttttctgcg ggagtttgcc aagcgcctgc atgagcgact gcagcgccag cagcttgcgg
     4981 tcagcgttga aggtgttgaa atccgtatcg tcggcagaaa atgctccgct cgtttcggtg
     5041 gcgccctccg tgcttcccgt gctgccgttt tcgaagccct gaccttgtcc ggaattgtac
     5101 gaggtcagcg ccgccagtaa tttggttttg tcgtccgtga aatccagatc cacgcgcatg
     5161 ttcgtcgcca gcgacaccag agccatcaaa tcggcgggct gcatctttgt accaacgaat
     5221 ttcttggcgg cttcgacgga ccggtcaatc tgttccggtt ccatcgccga aaaatcgaaa
     5281 aagagcaaaa tcaatcgccg gtcacgcgcg tccagcgacg gtgctgtttg ctttccagag
     5341 cgcagcagcg tcccccctgc aacacctgac accgtcgctt ccgccgcccc ggcggttgtc
     5401 agctcgtcta cattctcgaa atcgaaactg gaaatctcct gcttctttcc atcctcaaaa
     5461 atcgtgaagt ctcccttttt cagatcgcga acgaggttcc cgttcttatc gtgtgcaacc
     5521 acattcacca gcacgagttc actcgtaacg tgaatgcgcg cctcattcat tccggcttgt
     5581 cccgtctgct gggcaagcac gggcgccgcc gcttggaata gcagccctga tgcggtgaag
     5641 attgaaaata ttttcccggg aagccgcata ttctttttcc tcagaaccgg aatcgcgctt
     5701 gtattgtcac cctccgcatg ccgcctgtcc ccgtgacctc tccgaatgta aatgaattca
     5761 cgaccgtatt gatggaagtg aagcgcaccg tgttgaaaac attgttcgcg gagatgcgca
     5821 gatcgagcgc gcgggattct ttgatcgtga tcgtcttgtt cagcgacatg ttcatcgaaa
     5881 cttgtcccgg cccctcgata gtatttcgtc ccgcgttgcc aaaactggaa ccgttcgggt
     5941 tcacacatgc gacgctcggt gcgcaaaacg cagccgtatt gaaccatttg agcgcggttg
     6001 ggtcgcttat cgaaatcgat tgccccgcga cggcgtttgc gcggagcgac ccgctcacgc
     6061 cacggccgat gtccagccct ccgcccagca cccgcggcgt gaagtacagc ccggatccaa
     6121 tcgaaaaatc accgctccac tgccatccgc ccagaatgtg gctccattca cccttggtca
     6181 caaaccgccg gttgtcgcca actggcaaat cgtaaatcca gtttcccgtg aatttgtgcc
     6241 gttgatcgaa gctggaaagt ccgcggtccg cggagatgtc gaacggatct tgcgcgacaa
     6301 cgctgccgcc accgccaatc gatgatgcgt catcgatgga cttcgaaaac acatactgcg
     6361 cgctcagccc cagcctcttt gtcatgcgct tgcgcacgcg caccgaggcc gcatgcaaga
     6421 tcgagtttcc ctccgaagat tcgtaaataa acggctggcc gccggagacc accagcgcgc
     6481 gctccgtatc cagccgcgtt cctttcgcac cgttatagcc cgcattcaac accacgccac
     6541 caggcaactc ccgctgcaca tcgagattcc agatctgcac gtagccgagg cgataatttg
     6601 ggttaacgcc gaagttgttg gtcaccgtgt ttccggcagc ggggaaacca gttgtgagcg
     6661 tgagcgggtt cgtgccaatc agtccgttta catccgtggt atttgtcgcc gtgtttgcga
     6721 agggcggctg aaacgcgaag ttttggatca tgatgccgta ctgcgccagg ttgtagttga
     6781 ttccgtagcc cgccctcact actgtttgtt tctgcggttt ccaggccatc ccgatgcgcg
     6841 gcgcgaaatt gttgcggtgc ggccggacca gcgacgccgg aaatattcca ttgaagggtc
     6901 cagcatgacc aggcagcaca accgcggcgg atgtgaagcc cggagccacg tcgagattag
     6961 caatccgctc attcgcctcg gtgtaaggcc cgttgtattc gtagcgcagc cctaaattga
     7021 acgacagatt cgaacggaat ctccaatcgt cctgcgcgaa gaagtcaaac gcgttggcgc
     7081 ggaagttgta ggaatttgtg ccggactgca gcgaggtttg ctgcgggaaa cccagcagga
     7141 agtccgcaaa atcgtatccc gtgtcggtca ctggttgcgt gctcccggtt gtgtattgtg
     7201 acgtcgcgaa cccggtaaac acaaaactac cctccgcatt tcttgccgag cgaaaactct
     7261 gcaaaatgcg gcggtagtct cctccgaaat gccagttgtg ttttccacga ttccacgaaa
     7321 ctgtctccga gaaggagtag gtctggtcga gctcccggcg cggcgtcggg tcgctcagcc
     7381 cgccaaacga cgtgaaactg attcccggcg gtccccagtc aaacggatcg tgcgaaattc
     7441 cgccaatccc cgcgccacct ggtcccgcaa catctgtaac gttcgaatag agattggttg
     7501 tggaaatatg attgtggttg tagtttaccc ggaagatgtt cgtgacgcgg cctttgccgt
     7561 aggtccaccc cgcgctggcg ttcagccctt gcgttcccgt tcctcccgcg agcgaaggga
     7621 atggattcac gatactgctc gaactgcgcg accagttcaa tccgaaattg atattgttct
     7681 gcgaacgacg tctgcctccg ccgcttccgc cgcctccact ccctccgccg aacggaccga
     7741 atccgggacc gctgcttgcg ccgaagttgt ggatcaaacg caagatcacc gaatcggaat
     7801 tgctctccgc ggacgtcacg tagtggaaat tctgcccgga agccgtcgtc gcaatattcg
     7861 gcaacggaat gtacttcagc agcgccgttg cggccggact gatcaaggac ggatcgatct
     7921 cattctgcac accggtgaat tgatatggtt gcccggtctt aggattgaat atctggacaa
     7981 gcttgccgtc gttgtacgtc gctgcggaaa aattgccgcc gcgttcgtca ttcgtcggca
     8041 ccgtcgagaa cgcatcgtac ggcgagctcc cgcgcgaccc gttccatccg ccaaaaaaga
     8101 accatttgtt gcccccgttg aatatcttgg ggatgttcag tggcccgccg atgttgacgc
     8161 caaaacgaga ctggttgtaa ttcgctttgg gcgatgcaat gccggtaagc gaatacgatc
     8221 gcgcatccaa ccccgcgtta ttgtcagaga aatacagcac gccatgcggc tgattgatgt
     8281 tgaatccccg gggcatcctc ccaagggcta tcagcgcgcc gccgggcccg ccgaatcccc
     8341 cacccccgcc acccccaaaa ccagcgccgc cttcgcgttg catgcgatcg cgaaactcct
     8401 ggattcgttc ctgcaggtcc tgttcgctcc ctccgccaac gtcctgcgtc cgtccctggg
     8461 cgccactgat gctcaccgac tccgtcggtc cctccgctcc cgctccgttc atgggcaggc
     8521 ttgaaagatc gctgttgcct tggctcccgc cgcccgctcc gccgaagccg gagcttccac
     8581 cggcgagcga agagagtgcg ctatccgtcg ccaggctttg aaatccccgg cctgccgccg
     8641 ccattgccgc gttgttgctg ttcgactgtt cttgctggcg cgaagccaga atcaattcgg
     8701 cgtccacttt cgcggatgga ttctccggat tcagcaccac ttcctgcgtg aaagcagcga
     8761 atcccatgaa ctccacgcgt accacgtacc tcccgcgcgt cattcctgac aatccaaatt
     8821 tgccttcgga gttcgtcgcc gccgcgtact ttttccccgt caacgtgttg gccgctgtaa
     8881 ctgttactcc cggtagcggc gtcttcccgc ttcgagccga accattgatt tcatacgctg
     8941 gaactgcggc gggagtcgtc gcggtctgcg ccgtcggcgg cggcgaagcc ggtgccgcct
     9001 cctgcgcacg cgagctcgac gcaaaaagaa ttgcgacgaa gtggagtatg agaatgcccc
     9061 gagcaatcga cttcaaatct tcgtctccct gagtcaacta taactgcggt ggtttgcttt
     9121 ttggcgcggt tgcaggctta cttccggctg gcgcatttcc cgccgggccc tttccgttga
     9181 tcatgcccat ctcctgcagg cgcttccatt gctcaggaga aagaatcatc acgttcttcg
     9241 gcacaaacgc attgttctcc acagctccgc gcaccactac gtggtcgccg ggctggatgt
     9301 ccgccatcgt gatgctttcg cgccctctgc gaagcgaggt ctcctcgttc aactccagcg
     9361 tctgtgtgac gttgtccggt cgcaataccg tgagtttcgg cgcgtcgacg gatttcactt
     9421 cgcccacaac aatatccttt cccatttcgc cgaacccttg gccgccgccg cccgccccgc
     9481 caccggagcg cccgccgatt gactgcgccg tcacgctgtg atccgcattc tccttgccgc
     9541 gcaccaacac gaagtcgcca accttgaaat cttcccgttt cgcgccttgc cggtccttgc
     9601 ggtattccgt cttatccgta aacttcaccg tagccgtcga gccgtccggt ttcgccaact
     9661 ccaaggattc tttcttgatc gccgtaatct ttccgagcaa cggcaagccc cggccgtctt
     9721 ccggtctttg cgtcagtctt gcagtcggtg ttgctccttg gtcctgtccc agtgcagcag
     9781 gcgcgggcgt ctgcgctcgg ccggccgttg ccgcaactaa gaacagcaaa cttaagcgca
     9841 agaattgtcg cccgtgcgtc atcatgactg tcccccaagc aaaccctctc atctttaaag
     9901 acgtaagcgc aggcgtcaaa gtaacgccac ctcgcctttc tggccagcgg taattcctcc
     9961 acttcatgga ttcctcgaaa acgacactta agttcccggc cgaaggcgcg gcaagctcat
    10021 ctacttgtgt acatcgcggg cgagccaaat gtactttctt ctttgttgac tttcaactta
    10081 atctgcgaca tcatgcgtcc gttccaccga ggaagatccg cggcgcgcac ctgaaagcca
    10141 cctgccccca cacaactact ctcttatgaa tgtgtttggc agggcggatc agccgccgca
    10201 gacggaggat gattcaaaaa tgaatagtct gaagagtttg aatacccccc tgctcgcggt
    10261 cttgctgctc atcgccggta atgcaaccgc tcgcgcacaa accacgcaac agaccggtga
    10321 tcagggccag tccggcacca ccactcccaa gaagaaaacg gcggcggatg cggccgcttc
    10381 gaaatcgccg ggggctcccg catcggcgtc aagcgcgact ccgccagctt ccaccgcccc
    10441 ggccggcaag tcggccgccg cagctccagc aagcacacct ggcaacagcg ctccggcttc
    10501 caagccagat acaacgcaaa agaccccacc tgcgaagagt gcagccatgg tctgggtcaa
    10561 tacggattca ggtgtttacc acaagcccgg aacacgctgg tacgggaaga ccaagcaagg
    10621 gaagtacatg acggaagccg atgccatcaa ggcaggctac cacgcagcaa agaagcaata
    10681 agaagaactg ttcggccact agagaagcgc ggaatgcgaa aattctccgg gcgttttgtt
    10741 tttttcgcga tattttttgc ttcgcgcgtc cacttttctc gcggttattt ttcgcgcagg
    10801 atgacttcgc cgtggcccac cagttggtcg ttcagccaca ggcggtattt gtacgtggcc
    10861 gctttgcttc cggggcgcgg cgggccgctg agcagccgca tgccggcggg cgcctgaaaa
    10921 acgttcgacg aaaacggaca gcggttcaca tcgaattcca cgcgcaaatt gccggcgtcg
    10981 gagatccagg atacctggtc gccttcatcg atcacgacga cttgcgggat gagtccaacg
    11041 agtacggggc gttcagccat ttttctgtgc ggccctatgc cgttccgggg ccgatactcc
    11101 tgattctatc gctgcacagg gacgatagca tatcccacgg gaagaattct tgtgagcttc
    11161 gtcacagcgc ccgcgaagtt cctcttagag aaacctaatc ggcgattttt cacaaccaac
    11221 ccgtaccttc gggcatctat attaatgtaa cctgtacttc attgcgcttg accgttcttc
    11281 ggcgcaaccg tagactgaaa ttgttggttc gtggcgcgaa gttccggctg gatctcttgc
    11341 gctcgcggaa agatgcattc ataagcgtct caatctggac gcggaggatt ttgccggatg
    11401 gcttcagctt ctcgctattt gtctataccg ctgccgaacg gatcgcggat ttccgttctt
    11461 ctttctttgc cctttgtgct cgtccacctc gcctcgctgc tgattttctt cgtgccgttt
    11521 cactggtatt acctggtcgc atgcctcggc ctgcttgctg tgcgcatgtt cttcgtcacc
    11581 gcgggctatc accgctattt ttcgcaccgc tcttttaaga ccagccgtgt gttccagttc
    11641 gtgattgctt tcatggccac gagctcttcg cagaagggcg tgctgtggtg ggcggcgcat
    11701 caccgtcatc atcaccggca ttcggaccag gagctcgatt tgcattcgcc gacgcttttc
    11761 gggtttttct ggtcgcacgt cggctggatc atctcggaca agtacaacga cactcgtctt
    11821 gaatacattg gggacttcgc gaagtttccc gagctgcgct ggctgaacaa gtatcacctg
    11881 gttccgcccg tcacgctcgc ggtggtgatc tggctgatcg gcggatggcc gctgttcatt
    11941 tggggcttct gcctgagcac cgtgctgctg tggcatgaca cgttcaccat caactcgctc
    12001 tcgcaccttt tcggttcgcg ccgctacgag accaccgaca ccagccgcaa caactggctg
    12061 cttgcgctgc tcacgctcgg cgaaggctgg cacaacaacc atcatcactt tatggcttcg
    12121 gcgcgccagg gtttcttctg gtgggagatc gacatcactt actacaccct gaaggttctc
    12181 tcctggttcg gcatcgtctg ggacctccgc aaagtcccca cgcatctgct gacccaagac
    12241 cggctcgccg cctagcgcaa gatctacgga aatacgtggg acagacagaa atgtctgtcc
    12301 catttctttt ttcttgatgg acgagcacgt tccgtcccat aagcccggcc aagttggccc
    12361 aaccgacacc gtcggagtag acatccagcg tcccacatct aaagtaatac ttgaggagaa
    12421 tgggtagtca cccaccccca ccccccgggc atttgtatgg atgtgaaaag aaaggagttg
    12481 cgagaagggc aattcgtaag cgcatgaaaa caaagtggct gtttttcgtg cgtcagaggg
    12541 gggcaatttg taagtgtatg aaaacaaaga agaggggcaa aagcatagaa cggagtagtt
    12601 gggccgacgc gacgtggggc taactttcac aactcatgat agcatctatg tatacgaact
    12661 gtcaataaat ttgttgggta tcattcgaaa ggagcgggaa cgtgttgaaa aggctggggt
    12721 tgagggattg atgagagtga ttcaagggat ggggatccgg ggcgagaaag cgaaaaatct
    12781 tgagaaatga agattccgaa agaagaggga ggaaaagtca aaggccgcaa tactgaaaag
    12841 ccaaacccca aagggtgcgc cccccgattc aaaaacctgt gccggccgcc aagaatagtg
    12901 cggtgatata gggtaccctc caatcaacct gtacatgagc aggccacttt tctactccaa
    12961 tcggcgtcag agtttccagt aagatcgatc tctgagagcc atcctatgcc gagaaggctg
    13021 ttggtcgtga tgctagctgc ttgctccatc ctcgtccaag cgcagcaatc gtccacgcaa
    13081 gaaacttcac ctggccaaac gcaggcgcaa ctacccgccg ctcagcaaag cgaagcgaga
    13141 accaaaatca gcgtcaattc cgagctcgtc gtcctccctg tgaccgtcaa agatcgctac
    13201 ggcaatctcg tcccggatct gcaaagggaa gagttccgca tcttcgatga caacgttgag
    13261 cagtccattg aagttttcac ggccgaagcc attccgctct cgctggtcgt cctagtcgac
    13321 gacgacctga aatcgaagga cgccgcacag atggctcccg ctctgcgtgc catcaccgcc
    13381 gggatcaaca ccagcgacga agtcctcatc tgccgcttcg acctgtcgtt ctatccaggc
    13441 gatggtttca caggcgatct cgacaagctc cgggccgacc tgaaggatgc ccaggaccat
    13501 agcggccctt caacggccgg ccccgtcccg ttcgtcacac caccatcgag ccatgctcgc
    13561 ggcgtgggcg aaccgcctcc gggcccggcg actaaccttg gttcccgccc aaccaaagct
    13621 ctcgacgacg cggtccattc cgccgccgga ttacttcacg atcgtggagc gagtcgccgc
    13681 aagattatcc ttctgatctc cgatggtctc aatgggcccc aattcaaccg ccatacgtac
    13741 gccgacactc tcgaggctct ccttcacgac aatatatccg tatacagctt ggctgtcggc
    13801 cactccttaa agcgaaagtt ctcccgccta gtcaactacg cgagcgactc cggcggcgac
    13861 atctattacg ccgtccaaag cgccgccttg gaaaggctct attcgcgaat aaccgaagaa
    13921 gctcgccatg agtacaccct ggcctacgtg ccccgcggta acagccgcaa cgccaactat
    13981 cacaaggtag aagtccgcac cacacgagga gccgtcacca ttaagacccg ccgtggctat
    14041 tacaccggtc ctctcgtcga tcttcctaag caatagggca ggcgacgcac tctttgactc
    14101 tgtcgaggca tgcggaaccc gcgccatgcc gactcactga ggatcgccca ccggccctgg
    14161 cgctttttcg agggtgagtt cttcaaccgg ttacgcatct cctctccgcg atatactttc
    14221 gcgcatgcct ctccgccatc agttccacag aatttcttta tgctgcgttt ttttcgcctt
    14281 ttccgccgca ggggcggtgg cgcaatccac ccattattcg gtgaagctgg tagccgattt
    14341 cgagcgccaa ctcttgcggg gcgacgagac catcgaattt caagccgatg cgggggtagt
    14401 cgagtggcag aagcaagcag gcttgcgcgt tgtcagcgcg gacgttacgg atggagaggt
    14461 aaccgtggcc gagcaggcgg tgcgcgtgcg cttgccttcg ggtggcaggc acagtttgaa
    14521 gctcaagtac acggcggctg cagggcgagg catcaagtgg tttgcgggca acgcggggct
    14581 ggcgacggca ttttactgcg aggcgtggat ggtctgtgac aattcacccg gccagcgagc
    14641 cacgttgcaa ctcaagattg caatcccttt cagcccgctg acccacggcg ggagtggatt
    14701 ccgggtggtg ggacccgggc gccagggaaa ggagtggcgc ggccgcgatg gcgatcactt
    14761 tgtttttgag cagagcgatc cggtgcaaac gtatttgttc agttttggcg ttgcgcggct
    14821 tgcagttgcg atcgatggca tgttttcgat ttacgcgcag aatatggatg caaataaggc
    14881 ggcattcgcc aagacggcgg atgcctatgc gtttttgcga gccgcagctt cggtcgatcc
    14941 gatgaacgcg cactacacgc aagtgttttt gccggggccg agcatgggac aagaggctgc
    15001 cggcattgcg ctcatgtcgc agaacgacct taatgatctt accggcaaag atgacgtgca
    15061 gttggtggcg catgagttgg ctcatcaatg gtggggcgtg acgctgggga tccgctcgtg
    15121 gtcggatttc tggctgaacg aaggctttgc ggagttgatg tccgatgcct atatcgaaaa
    15181 gcaccaggga cgcgcggcgt acgaccagca aatcgcggaa ttgcaggacc ggatgaagaa
    15241 attgcgcgaa gaagggaagg atcgcccact gcattgggaa aagtggaagg acgcacacga
    15301 ggcgctgggg caaattccgt acgtaaaagg agcgttattc ttggcccggc tgaggacgga
    15361 gctgggcgac gagattttct ggcgggggat cggcttgtac acgtccggca gcgtgcgccg
    15421 gcttgttgac tccggcgatt tcgagcgggc gatggaaaag gcgagcggcc gcgatctgaa
    15481 agcgctattc gatgaagcgg tctatcactg aaatgaggct gattcgatga tactgccggc
    15541 ttaagggccc gtttgtctgg gtttcgttaa cacttgcggg gtgtttggcg tcttggctgg
    15601 atgtaaatgg cacaccgaac gccgacggcg tcaggaaggc cagccatgct acggaaacca
    15661 ttgctcttaa cgctctacgc tctgatcgct tgctgttttg ccatctcgtc tcattcgctg
    15721 agcagagagg cgcgccctgt ctatgcgagt cttacggccc acgaatgggg cacttttacg
    15781 tccatcgctg gcagggacgg cagtgccgtg gtatggtcgc cgcttaccgg gtccacggac
    15841 ctgccctgct tcgtggagca ttttcgcaat accggcttca agctcggcct gcgcggcact
    15901 gtccgcatgg aaactccagt cctttatttc tatgactcgc gcgaagagac catttggtca
    15961 gggtggcttt ctataagggt gtcatcaccg agtggtatcc tcgcgccagc cgcgttgagc
    16021 ctgccgcgaa tatttttgat gccaccctct accagcaaca cgctgacggg agcattgcgt
    16081 gggactccgt gaccctctcg ccaacgctga gaacggaatt tccgcgcgaa aatccggaca
    16141 atcactatta tgccgcccgg atgacctctt cgacgccgct tcgcgtgaag acccccgcag
    16201 gcgagcaaca agagaagttt cttttctatc gcggcgtgtc cacttttccc gtgccccttt
    16261 cggcgaagat gacagccgcg ggcaagttgc tgatcgaaaa tcgcagcaac gaggagtttc
    16321 cgaataccat tctatttgag cgccgcggcg aaaaagtggg ctaccgcctc ggtggcgcgc
    16381 tgcaaaaaga aatgaacctg gatgcgccgg agctgactgc ctccatcgaa aatcttggca
    16441 gagatctgga aggaatgctc gttgctcagg gcctttacca ggatgaagcg cacgccatgg
    16501 tcgaaacctg gagcggttcc tggttcgagg aaggtagccg gttgctgtac atcgtgccca
    16561 cggcatttgt ggccggcgtg ctgccgcttt cgattaatcc tgctcctgcg caaaccgtac
    16621 gcgtcttcgt cggacggctc gaaatcgttt cacccgcgac ggaaaaggca gtggagagag
    16681 cattattcac gggcgacagc gcgacactga agatgttcgg tcgattcttg gagcccattc
    16741 tggaaacgat gatccagaaa gaatcaaatc cagccagggt ccagcagttc taccaagccc
    16801 tgagtttcta ctacagttca gaaattgctc agaatatacg ccgagactga attctcgaaa
    16861 catcatgttc gctggtcgtg cagctgtcaa agggtctcag cgccgcgccg gcaactgcag
    16921 ctacttgccg atcagagtta gctgactggc aatgcaaagc cattggccgt ctttcttgat
    16981 ccacgtatcc gtgaagcggc cttgatgcgc atagggcttt cccttttccg tgcccttctc
    17041 gcgcgttgca ccgatgacga tgacagtatc gccgtggcgg tgaagcttca tgtccgtgga
    17101 cacttccacc gtgagctggt tagaggtatc gcggatggac tccaggaatt gggtcttgga
    17161 catcacggtt ccatcgtagt cggtgagtac aaaatcgctg tctagcattt ttcccatggc
    17221 gtccgcgtca tgcttgatct gcatttgatt ccaaagattt tccagcgcga tgattttcgt
    17281 ttcgtcgcct tgtgctctgg aaatgtcttg cgcaaatgga aaggacgaga gcgcgagcag
    17341 aaaaactaaa ggtcgcaaca tgacgataac tcctttgggc cgggtgttcc ttcagaggat
    17401 ggcacgaatc atgcagtttg tggctgcgga aattccaaat ctgcctgtgc cagataagag
    17461 gccgcaggcg agcagcaccg gccaaattgt cggttgcgcg ggtgtgggga ttgcgcgaaa
    17521 attaaggttc ccgccgtggc aggaaaagtc ccccgaaagc gccttcgcgg gcggttcgag
    17581 cttcaagagc gagaatcaag cgcaagaaca gagaaggaca aaacgtcatg acgcaacacg
    17641 ttggcgaact caatcctccg gtgcggttga tgctgacccc cggaccgtct tccatcgatc
    17701 cgcgcgtgta tcgggcgatg gctacgccgc tggtggggca catggacccg tggttcaagg
    17761 gttgcatgga agaaacgcaa attctgatgc gacagatttt tcaaacggag aaccggatca
    17821 ccatgccgct atcggcgtcg ggatcgggcg ggattgaagc gtcggtgctg aattcgctgg
    17881 aagaaggcga cgaaggcatc gtggcggtca acggagtttt ttccgagcgc atgtacgaga
    17941 ttgcgacgcg ggcgtcgtca aaagtcgtaa aggtcgaggc gccgtacggg aaaccggtgg
    18001 atgcggaaga tgtgcgccgg gcgggccagg gcaagaagat caagatcata ggcttcgcgc
    18061 atggggagac ctcgactggc gtggtcacgg gcatcgatgc ctaccgaaag gtcgcggacg
    18121 aattgggggc gctgctgatt gtggatacgg tggcctcgct cgcggccgta ccgctcgatg
    18181 tggataagca gaggatcgat atctgcttca gcggctcgca aaaggccatc agcgcgccac
    18241 cgggaatgtc gccgatcacg gtgagcccga ccgccgagga agtgttccga aagcggaaga
    18301 cgaaagtgca gagctggtat ttcgatttga cgacggccat gaactattgg ggcaaagacc
    18361 ggctgtatca tcacacgccg ccgatttcgc tgatcttcgc gctgcgcgaa gcgatgcgac
    18421 tggttatgga agaaggcctg gaggcgcgct gggagcggca tcgcgtgaat cagcttgcat
    18481 tgatcgcggg catcgaggcg atgggaatgg atctgctggt gaagaacccg gcggaccgtt
    18541 tgccaacggt cacggcagtg atgattcccg gcggcgttga cgatgcgaaa gtgcggaatc
    18601 agttgctcga ggagttcaac atcgaaatcg ccggcggatt cgggccggtg aaaggcaaga
    18661 tctggcgcgt ggggttgatg ggctactgca gccagaagcc ctttgtgctg ctgttcctgg
    18721 ccgcgctgga aaagtgcttg ctcgatcagg gcttccgggt gccgagtggc gcgggagtgg
    18781 gagcggcgat tcggagttac tcacaggtgg agacggctgc ggcggtcagc aggtaagaaa
    18841 atagaaatta gaaagtagaa gacataaagg gagattgcgt gaccacacgc ccacaactag
    18901 cgaattccgg cgccatgagc caattcatcg gcgcgttcga agtaacttcc aaattaagcg
    18961 ggcaaacgta tcagtgccgc ttctcgcaca tgtggaacgg catagcgacg cgccacgcag
    19021 acacgattga cacgaagttc tttatcgatg gcgaggccca tgtcgttgga ttggcccaca
    19081 ccgcgttcgt gaagttccgc gagaaggcag ggcgcgactt gacggaccgt gaagcgagct
    19141 ttgtcgccgc ggagtatttg cgcgagcggc tcgaggaaga agatgtgcgg ccgatctacg
    19201 acgttcccga agccgaagtc cttcgcctaa tcgcgcaggt tggcatcaag tagaaatcat
    19261 caacaatgga ttgggaatgg gcgggcttcg gcccgcctct ttttttagcg gtcagtcgcc
    19321 gggttcggcg tggaaagcgg cgagggtcgg atggcggagg ccgtcccggc gaatatagtg
    19381 gatgacgaga agcatgagca gcgcgccgca aaccataacg gcgacggcga tttgcagggc
    19441 cacctgcatg tcgtgaagat ccgatatcct gccgaccacg agcgggccga ggccgccgat
    19501 cagttgcgtg gcaaacatgt agacgccaac ggaagtggcg tgcgcgcggc gcggcatcat
    19561 gtcgtgaaga acggcagtga ccgggccgtg ataccaggac atgaagaaga ctgcaacgga
    19621 gagaccgaag agcacagttg ctttttcatc cgcttggata gccagcaaaa gaaacggcgc
    19681 cgcgagcaaa aatgccaccg cgatggccat gatgcggcca tacgcaagtc tccggtgcag
    19741 gcggtccgca acaatgccgc caatcaagac gccgaagaca gaagcaacca gcgcaatcag
    19801 cgcgagcagc acggatgctt cgcgcaaagt gaagtccttg taggtcttcg cgaaatcgac
    19861 gccccaggtg agaagagaaa cagtggaaaa cgtgatgcaa atcccggcag cgatcatggc
    19921 aacgaaagct ggaacactga gcagccgggc aatgggcacg acttctgaac gcggaccgcg
    19981 gggtggctct tctaatccga agagagcgag gcccgggaga atgccggcga gcgcaacgag
    20041 gaagagaacg tactgccagc cataccttgg cccgatgatt ccaccaaaag cctgaccggt
    20101 cgctccgccg agaagcatgc cggatgcaaa aatggcctgc gcaaaggcgc gccgctccgc
    20161 agggaacgcg ccggaaatca tggattgcgc ggctggggcg taagcagctt cccccagacc
    20221 gaccattcct cgcgcgatga agaagaatag aaaactggat accaggccac cggcgaacga
    20281 agccacgctc cagaagaaga tgcccagggc aatgatcgat ttgcggctaa aacggtcggc
    20341 aagaaatccg aatggtatgc tgccggcggc aagcacgacg aggagcgagg cctgcagcga
    20401 gccgagttgg gcgtcggtag cgtgaaggtg atcgcgcaac agggggatga gggggataat
    20461 cgcctgacga gccgcgaagt tgacaaaatt gatcaaagca agtacagcaa ggagacgaat
    20521 ctggtaggaa gtgagcggga tggacttcgt agacatgcta aattggctcg gtgacccgcg
    20581 cgtgacctgc tggagcgtag accggaagtt atcagccttt gcccggccta tcaagtttat
    20641 atgatgaata agaatactgc cccctctgcg aacgcgacga ccagtgaccg tttaaccacc
    20701 gttctttcct acggtgtgtt gctgctgctc atctacctcg ttttccggat ttacgaaccg
    20761 ttccttgcgg cgctgggatg ggcggccatt ctggtgattt ttttctatcc gatgcaccat
    20821 cggctcctgc ggcgtttctc cgcaagcaag gcggctgtat tgagtaccct ggtggtgacc
    20881 ttccttctga tcgtcccggc aattctcgtg accaccttat tcattcgcga ggcgttttcc
    20941 gtttcgcggg gcgtggagca tacgttgcag gtacaggagg cgcccttatt ggcgaaaagc
    21001 tgggcgtgga tcgcgcacca tgtgccgggc ttggatccca atgccgacgt gcttgagatg
    21061 ctgtctcagg gcgttcagaa agaggcgggt tttcttgcgg agcggctggg caccatcctg
    21121 cggaatatcg cagcgttcat cttcgacctg ttcgtgatga tcttcgcgat gttctatttt
    21181 tttcgagacg cggaccagat tattcgcgga gtccggagca ttctgccttt tgatgcggaa
    21241 catcgagaga cgttgatgac gcaaacgcgg gaccttatct ccgcgagtgt aaccacaagc
    21301 cttctcctcg cggcgcttca aggcgtcatc ggcggactcg gttttgcgct cgtgaagttg
    21361 ccggcgtcgc ttttctgggg cgtggccatg gccttctttt cgctggtgcc ggtcgtgggc
    21421 tccggactga tcttcgtgcc tgcggcgttg tggctggggt tcaccgggca ttgggggcgg
    21481 gccattcttc tgctggtgat ttgcgcggga gtgtccgcga tcgtggacaa cgtcttgcga
    21541 ccgttcttgc tgggcgggcg aaccgaattg agcgggttgg tgatcttcat cagtgtggtt
    21601 ggcggtgtcg gcctgttcgg catgttgggt ctggtgctgg gaccgatcct tgtcgcaaca
    21661 gcggcggggg tcctggcggt atacatggaa cggcctggaa acccgccaat aacggctcga
    21721 taacaagcgg tttgtcttgc cgtgggagcg tgacttgagt gtgctagagt aacgcgctcg
    21781 aaggaatttc atgacgacca aacctgcccc gaaacccacg gagcttcccg ccgcgaaact
    21841 gaggtggagg tgcgagcttt cgcgcatccc cttcgaaaca accgcgcaag ccgaactgcg
    21901 cgaagggttc attggacaag agcgcgcgct gcgcgcactg aagatgggcg cggagctttc
    21961 ggcgcccgga tacaacgttt ttgtgtgcgg attagcgggc accagccgcg gcggaacgat
    22021 cgcgcgcatg atcgaggagc ttcacccgcc gacgaaagag tcattggacc ggtgctacgt
    22081 caacaatttc aaattgacgg accggccgcg gttgctggcg ctgccgcgcg ggcaagcgaa
    22141 cgccttcaaa aaagacgtgc aggcggggat cgattttctg cgccggcgga taccgcaggt
    22201 gttcgaaggc gagccgttcc agcggcaaaa agggcgaatc gtcgaacgct acaccgtgcg
    22261 cgaaaaagaa ttgatggacg atttcacgcg caggatcgcg cgcgaacaat ttgcgctggg
    22321 ccacatgcag gtgggcgcgg tggcgctgcc ggaaattttt ccggtgctcg aaggccaaat
    22381 ggtaccgatc gaggatatct ccaagatggt gcacgagggg aaactcgaaa gccccgtggc
    22441 ggaagatatc gagcgaaagt acgagcagtt ccgccaggaa ttcactgttg tgtaccgaaa
    22501 gacgctgacg ctctcgcgag agctggccag cgagttaagt tacctggagc aagaagcggc
    22561 ctcggtgctc gtggatggcg tcatcgaaga actcaaggaa aaatacgccg gcccaaacat
    22621 cgccgaatat ctcgaagaag tgcggcacca tcttctcgac aatctagacc cgttcaagga
    22681 gcacgagggc gaaggcgaac acgacgaaga aacgccggac gggttgccca aggcgccggg
    22741 aagtcccgag cgagatccat tccgggtgta cggcgtaaac gtgattctcg cgcatagcga
    22801 cgaagacaaa tcaccggtca ttttcgagac cactcctacg tacgccaatc tgttcgggac
    22861 gatccagcgc gcgtatgaca cgcgcggcgg atggtcgagc gacttcatgg atttgcgcgc
    22921 cggttcgctc ctccgcgccg atggcggctt cctaatcatg tattcgctgg aagcgttgtc
    22981 ggaagtgggt gtgtggcgcg cgctgaagcg cacgctgaac cacaaccggc tggaaattca
    23041 gccgctggaa atgttttatc cgttcggcgg gagcgcgcag aagcccgaag ccatcgacat
    23101 caatgtaaag gtcatcctca tcggtgaccg ttccttgtac gaactgcttt acgagtatga
    23161 ggaagacttc cgcaagattt tcaaggtgcg cgtcgaattc gacgaagaga tggccatgag
    23221 cgacggcgtc atcgcggaat atgcggggcg gctgcgcgcc ttgtccgaaa aagaaaactt
    23281 gtacccgttc gatcgcggcg cgtttgcggc gatgctcgag tacggtgtgc gccaggcggg
    23341 gcgaagaaac aaggtgacgg cgcgcttcat cgatatcgcc gacctggcgc gcgaggcgca
    23401 ttacaacgcg gcggcggccg gggaatccgt ggtccgcgcg gcgcacgtgc gcggcgcctt
    23461 gtcttcgaaa atggagcgcc acaacctgat cgaaacgcgc atccgcgaaa tgattcagga
    23521 aggaaccctg ctcgttgatg tggaaggctc acgcgtcggc caggtgaatg ggctgagcgt
    23581 cctggaaatt ggcggatact cgtttggcaa gccggtgcgg atcaccgcaa cggcggctct
    23641 tggaaaaacg ggactaatca atatcgagcg cgaagcgaat ctgagcgggc gcttccatga
    23701 caaagggatg cacatcatcg ccggttactt gcgaagccag ttcgcgcagg acaagccgct
    23761 ctcgctcgcg gccagcatct gcttcgagca atcctactcg ggagtcgatg gcgacagcgc
    23821 cagctcgacg gagatctatg cgctcgcctc ggcgctttcc ggattgccgc tgcggcagga
    23881 tatcgccgtg accggctcga tcaaccagca gggcgatatt caggccatcg gcggcgtcaa
    23941 cgaaaagatc gaaggtttct tcgacgtctg tcgcatcaag gggctcaccg gcacgcaagg
    24001 cgtgatgatg ccggagtcca acgtggaaga tttgatgctg cgcgaagatg ttctggaggc
    24061 cgtagcggcg ggaaaatttc acgtctggcc ggtaggcaaa gtcgaacaag gcattgagat
    24121 cctcaccgga atggccgccg gaaagaaaaa cggcgacgga aaattcgaac cagggaccgt
    24181 gctcgcgatg atcgatgcgc ggctaagcga aatggccaag accctgaaag aattcgaata
    24241 acctcgaaat aagctcgctc tacttgcctt tcgggccgcc gcgacggtcc ccccacgcat
    24301 tttcatgatg cgcgtttatg gggctggatt ctgcttggcc gtttgctgaa gcgagctcac
    24361 tgcggaaagc ctacacgtcg aagcaaatct tggaaacgcg ggtctgagcg cagatcatcc
    24421 atatgcgggt ccactttcag gtatagaatc cactgatctc ggtccgtgta tccctgttcc
    24481 aaagacgcga tcgcctgatc tttttcgccg agtcctactt ggacaagggc gataagacac
    24541 ggcgatacgt accgatgctt cgaccgctcc tccaattctt cgagtagttt tctcgcttcc
    24601 tgttttcttc ccgaatcgcc tagaaccttc cccagacttg ccacgatgag ctcatgtctg
    24661 ttgcccaagt tcactgcctt ctccagctcg gcgatggcct ctttgtacat tttcttttcg
    24721 tcatagatcc agccgagacc tacatgtgcg tcggtatagt ccggttccag ctccaaggcc
    24781 ttctgaaatg caccaattgc ctggtcgtac tggcgtgcgg tatagaagat atcgccaagc
    24841 tggctctcac tgccggagct gagcggatcg agcagtattg ctagtcgcat ctgtacaaag
    24901 gatccctcat ttcgccccat ttcttgcagg tattcaccat accaattgtg ggccatggcg
    24961 tcatttgggt tgagttccag ggccctccgg tattccttct cgacggctgg ccagtcccag
    25021 tcgtaaagtt ggattacgcg cgccagcgcc aagtgtgcct ccccgagaga atcgtcaatt
    25081 tccaatgcct tcgtggctgc cgctttcgct ggcaggaggg cctcctttgg ggacaggcca
    25141 ccgacccagg gctgacccaa ggagatataa gactcggcaa ggccggcata agctaaggca
    25201 tagttctggt ccttctgaat cgcctgctgg aagtacgcga tgcctttcct cacatcgcgt
    25261 tctctctgcg tagataataa aaagcgaccc tgcagatagt cttcgtaggc ttcggagttg
    25321 atggtgcgag tgctcgccag ccggactttc tcttgtggcg tcagcttcgc tttgatctcg
    25381 tccgcgatgg cgtgggcaac ctcgcgctga agcgccagga catcgcgcag gccgcgctca
    25441 taggacttag ccaacagatg gcggtccgtc ggcgcatgga tcaactgcac cgtgatttct
    25501 accttgtcgc ccgagcgctg caccgcgccc tcgaccacgg cgtccacgcc cagctcttgg
    25561 gcaatttgcg gcagtgattt ctttgttccc ttgtattgca ccacggaggt attcgaaatc
    25621 accttgagcg cgctgatctg cgctaattcg gtggtcagcg cctcagtcat gccgtcggcg
    25681 aagtattcct ggtcggggtc gcgagagaga ttttcgagcg gcagcacggc gagcgattga
    25741 atggcaccga gatcgggtct tcccaaaaat cgctggcgca gcccgccgcc atacaagcca
    25801 ataaataagg ccaccagaaa agcgcaggcg atgaaggcga gccaaaccgt ccgccattga
    25861 gttcgtgttc cggccggtgc gggaacctcg gcggctggct tctcgaccct caggtctgtg
    25921 gcttgctgat tggcagcagg agcatcctga tttgtagagt cgggcgctga cgccggcaac
    25981 gcgcgctcaa ccggagcaat gaaacgataa ccgcgccgcg gcacagtctc gatgaaacgg
    26041 ggactattgg cgtcgtcgtc tagcgcctcg cggagcttat tcacggccgc gtttaggcta
    26101 tgctcaaaat caacgaaggt atcctccggc cagagccggg cgcggagctc ttcacgcagc
    26161 acgacctccg caggtcgttc gagcagcagc cccagaatct caagaggctg cccgtggagc
    26221 cggattgtgt gtccgtgctt gcgaagctgc ctggcgcgag aatcaaactc gaaggcgcca
    26281 aagcgcttgg gtagggccga ttgtgtagtc tccgtcacga cccaccccta ttcgagcgaa
    26341 gtctaacacc ccaaagggcg ccaggcaaag gctgcgtcct ggtcctcgtc cacatgatta
    26401 ctggctcttg gaaccctctc gttgcgctca gactccaagt cgctcgcttc ggcgcaagta
    26461 agttacttag tttcagcagg atccgcgatg acctcttctg accatcaaat gagccgccgt
    26521 gcattgccac gccggggctt gctgtggcat taccatggat acacgacacc gtacttgatt
    26581 caggaggcca cccgtgaata cgactcttcg gttcctggtt acggcattgg cggtgttgct
    26641 ttcgctggga gcagccagcg cgcttgccga cacccgcgca acgccgaaac ccgaaccgga
    26701 ggtaagcttc tccacaccga ggcttcattt tagctatcaa ctggtgggca ccagcagccc
    26761 gcaaatgagc gaggtggtca cgaactcagg caccgcgccg ctggtgatca ccgctatgca
    26821 tgtgaccggc gaagacgccg ctgatttcgc catctcttcc aatttcccac tgccggtgac
    26881 agtcctgccc gggcagagcg tgacggttaa cgtctcgttt gcgccgggtg agccatggac
    26941 tcctggaacg cgccgggcga gattgaaatt cacggacaac gacgggcaac agttcgtcac
    27001 cttgaccgga atgggtgtga attgtggggg gccggtgcct gcggtttttt cgaacggcat
    27061 ctgcgccgat acggacctgg acggtttgaa tgacgtctgg gaggacaacg actacatcga
    27121 catgaacaac aatggccgct atgaccccgg cgtggacttt aagttccccc acaaggcgcc
    27181 tttcgttttc tccagcgtga ccaaggcggg cagcggctcc ggccgcatgt tccccacggt
    27241 taccgacccc ggcgtcgagg tccgtccttc caccgtggtg gtaacggttg ccgccggtgg
    27301 cgaggtgggc gcggcgacgt tcacgtactc agtttcggct aatgaagagg gcagtggttc
    27361 aggtgatgac gcgagtaccg ggggaccgct ggaaatccgg ccggtggtga atatcggcgg
    27421 gaatctgcga ctgctcttct accgttcaag ccctacggaa agcttcgttg cgggagatac
    27481 gttttcgttc accgtaggga tgggagaaga gaccaaagtg gtggacaaga atgttcccaa
    27541 catttttgtt cagtacgact acatggacta cgacgtaccc gggaatgcct gtgtggctga
    27601 tccagattgc gaccagggtg gatttcaatt gaatgatgtg tgtcacagcg gattctgtac
    27661 ccacaatcac tttccaggtg acccgctgtt ccgcaaggtg gtggaccagt tcgccgcgca
    27721 cgggattacg ctgtacatcc acccgtctcc caaagcggtc ccgcacgcag cggtggtcac
    27781 ctgggcgaag agcactgacc agggcaacgg accgcacacg tcttgtgcgg gcgacgaggt
    27841 gccggcggtc ggcatcaccg gcagccaggc gatcaacttc cacaacatta agaaccggcc
    27901 aggctccgac ttcgccttgg acccgagccg aaagatgatt tatcactacg ccgtactcag
    27961 ccacgccagt atctgcttga ccgatattaa tggtcccgtc ggctattgcg gtgcgtgttc
    28021 gcaagaccgg agcacgccaa gcggttttcc cagggcgggc tcttcgggga actccgagct
    28081 gcccggcaat gacttcatcg tctccttggg gccatcgctt aacaacgcgt tgggctcggt
    28141 tcccagcagt ccgtttattg aggaaggcat tttcatgcac gagctcgggc acaacctcgg
    28201 cctgcatcac ctcggtgacg tacccgcgcc cgagagagcg ccaaattatt tgagcatcat
    28261 gaactacaaa tattctttca ccgggattca gcacgcggcc acaccgggaa gcaacgtccc
    28321 agtcgaggac ttgcgcgagt tggactattc ggagcatacg ctgaacaccc tggtcgaggg
    28381 cagcctcgac gagaccgccg gtacatctcc gctgtcctcc ggctacaccg gcatcgtccg
    28441 ctttttcaat ggcgtggggg gcaatgccag aggcgcggag gccggcccga ttgactggga
    28501 tggcatcaac gggattgacc ctaacccggt aagtgttgac atcaacaagc tcaacggtac
    28561 caccgagacg atggtcggct atcgcgactg ggaccatacc accgggcaat ccggctctgg
    28621 cggcggagca tgcaccacca gcgcagattg ccgcgtgaat gcgattcggc agctttactg
    28681 ggctaatgtg gaccggtcca tcgataccca cgagccgtgt ttgaacggcc gctgccagtc
    28741 cttgtggctg aattttcaga atacggcctg gggcatagcc gatgggctgt cgacgggttc
    28801 ctcgagaacc attttcgaac ccggcgcgcc gggcgcgtcg gctgccccaa agataaacgt
    28861 cagctatgac aagaccctcc tgcggggggc gccggacgcc atcatcacca ttgtcgagtt
    28921 caccgatttt cggtgtgcgt tctgccggca agccgaaagc acgctgaagg aagtcctggc
    28981 gaagtaccca ggcaaagtcg ccctggcgca caaggattat ccgctgggag ggatggactc
    29041 gcagagcgcg ctggcatccc gggcggcgcg ctgcgccggt gaacaaggca aattctggga
    29101 gtaccgcgat ctgctcttgg gctcgcccaa acttgacggg gaggtgctcg ccagttccag
    29161 tcaaaccctg aatctcaatg tgaaagagtt cgattcctgc ctagccgccg agcgctacaa
    29221 ggcagcgatc gagcaagact tgaacgacgg caacacggcc ggggtctcgg ccacgcctgc
    29281 attcttcatc aatggcgtgc gcgtgaccgg cgcacagccc gcgagcacat tcgaaaaaat
    29341 catcgaccaa gagctgggtc gtttgactca aaacagtcct ggctactgat ccagttaagc
    29401 tgcaacccaa agtgcgtcaa gcaaagacag ccttggctgc tgttctgcaa gtaagttact
    29461 tagtttcagc aggatccgcg atgacctctt ctgaccatca aatgagccgc cgtgcattga
    29521 ggtagcgcgc aacatacgcc aaagttgcgg ccatcgcgcg agccccacgg ttaaatccca
    29581 gcgttcgcaa gcgaaattta gtttcaacaa gaggtttcac ccatgaaagc tattcttcgg
    29641 ttcctagtta cagcacttgc gatgatcctt ttcgcacgta ccggcctgca ggcccaaaac
    29701 gtcgtgatcg attggaacaa tatcgcctca acgactatca ttaccaacgc gaaggagtcg
    29761 tccaccgctt ccggcgtctg gtttgcctat gtgcaactcg ccgtctatga tgcagtgaat
    29821 tcgatcgacc atcgtcacca tccctatctg ttttccaccg atgccccgga tggtgcttcc
    29881 aaggacgccg ctgccgtagc cgctgcccac cgagtcctgg cgaactactt ccccacacag
    29941 caggcgagcc tggatgcgca atttgcatct tcgctggcag cgatttccga tacgcctgca
    30001 aacatctcag cgggtaccgc ggttggagaa caagctgcgc tggctctgat tgcggctcgc
    30061 tcccacgacg gcttgcttgc caacgtcccc tatgcgcccc cagtgggccc aggattctgg
    30121 caacccacac cgccaaagtt tgcggccccg ctcacacctt ggctcgggca gatggtgccg
    30181 ttcacaatga ccagcgccat gcaatttttc cccgacgaag gtcccgacgc tctcaacagc
    30241 gaggaatgga tcgatgacta caatcaggta aagacgctcg gtgctctcaa tagcgccgtc
    30301 cgtacaccac agcagaccga aatcggcctg ttctggaccg aacacaccgg acagcagtac
    30361 gcgcgcgcct tccgcaatct ggcgacgctg aaagagctca atacctcaga taccgcgcgt
    30421 ctcatggcga tgctctggac tggctacgct gattcggtaa ttggctgctg gaatgccaag
    30481 ttctccttca gtttttggcg tcctgtcacc gccataaggg ctgctggcgg caatccccag
    30541 ctcacgggcg accctgcctg gacgcctttg gcagcgactc ccgcccaccc ggagtatccc
    30601 gctgcacacg gctgcgtcac cggcgctgtc tcgacaattc tcgcgggcta cttcggtacg
    30661 ccgaaagtgc agttctccgt agacagcctt gtcacgcaca cgacgcatac ctacgacagt
    30721 acaaacgacc tgatggaaga agtggaagcc gcccgaatct acgcgggctt ccattatcac
    30781 cactctgtgg tcgaaggacg agagctgggc cgcaaagtag cacatcagtt gatgaaagaa
    30841 ttcttccggc caagccatga agaccgtgag gagaatgcag aaattggcag ggacacgaac
    30901 gcagagcaca ggaactaggc tgctttcgaa cgaggagagt catcaaagcc cggctgggaa
    30961 tggtctgggg aacgcccctc tgtctggcca ttcccattcc gccggtaaga ttctcttcac
    31021 cagattaaag tagttacgcg aacgtcggct gggctccagg tgctgccaca ttgtcgtatc
    31081 acgggctacc tgtggaagtg aaattcctga catgcctcca acccaagtcc gcttgtgaga
    31141 agttaccgct gtatgggtca cttggtcgca aagacgccaa accggaaaca gatccgctac
    31201 ctaggcgcgg gagccagata ggcttggcga tgatccacac
//
LOCUS       DASYVO010000424         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_95383, whole genome shotgun sequence.
ACCESSION   DASYVO010000424 DASYVO010000000
VERSION     DASYVO010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95383"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1756
                     /locus_tag="VGR03_14275"
     CDS             <1..1756
                     /locus_tag="VGR03_14275"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV1995494.1"
                     /translation="TGSFFRALAVTMSVSLFTSLLLALSWTPLLSQYFVRPKETPVAE
                     SESLSGTDAAALLVAEEAHLSGFFGRIVAFYARAMEGVLKRPLLLAGSSVVIVILAFV
                     CYKALGSDLLPEMDEGGFILDYFTPPGSSLAESDRILQRIEEIVRSQPEVESTSRRTG
                     LQLGLAAVTEANRGDFSVKLKSDRKRDIDEIISDIRSEIEQTEPAAKVEFIQVLQDMI
                     GDLTSQPQPIVIKLFAQDPKLLANTAPHVADAIQKIHGVVDVLDGIENTISGPATTFQ
                     INPAVAARAGFTPEEIAVDAAAILEGEPASTPVVVNARAYTIRVRFPEQARATLDRMS
                     NTLLTSATGRTATLGSLATVSSDPGQTEIRRENLQRLVEVTGRFEGVDLGSGITAVKK
                     IVNGLHLPSSIRVEYGGTYAEQRKSFRDLLMVLVLALVLLFAVLLFEFRTFSAPTAIL
                     ASALLSTFGGLLALLVTRTTFNVASFMGMIMVIGIVAKNGILLLDAEHRFRDLGFSAE
                     ESMIQAGRRRLRPIVMTALATVAGMLPLAFAIGKGSQMLKPLAITVVGGILSSMVLSL
                     IFTPAIHFYLQPKTPAEA"
     gene            1971..2090
                     /locus_tag="VGR03_14280"
                     /pseudo
     CDS             1971..2090
                     /locus_tag="VGR03_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010332599.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
     gene            2178..>2736
                     /locus_tag="VGR03_14285"
     CDS             2178..>2736
                     /locus_tag="VGR03_14285"
                     /inference="COORDINATES: protein motif:HMM:NF042870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIL restriction-modification enzyme MmeI"
                     /protein_id="HEV1995495.1"
                     /translation="MPKKRQETANRKESQGTRPVLSLPEFVARWKASTLTERSAAQSH
                     FIDLCEVLGQQHPAAADQTGESYTFEKHVSKLRGGKGFTDVWKRGHFGWEYKGKHHDL
                     QAAYIQLADYREDLENPPLLVVCDQNQFEVHTNFTGTAKRVYRFTLDELLSDLPTANC
                     ALPPLEVLRALFTNPDELRPEKAATR"
BASE COUNT          546 a          883 c          693 g          614 t
ORIGIN      
        1 gaccgggagc tttttccgcg cgctcgccgt caccatgtcg gtatcacttt ttacatccct
       61 tcttctcgcc ctgtcatgga cacctttgct gagccagtat tttgttcgcc ctaaagagac
      121 acccgtcgct gaaagtgaat cgctatccgg cacagatgcc gccgctctcc tggttgcaga
      181 agaagcccat ctctccggtt tctttggccg gattgtcgcg ttttatgccc gcgcgatgga
      241 aggcgttctc aagcgtcctc ttcttctcgc ggggagttcc gtcgtcatcg tcattctcgc
      301 gttcgtttgt tacaaggcgc ttggttccga cctcctgccc gaaatggacg aaggcggctt
      361 cattctcgat tactttacgc cgccgggaag ctcgctcgcg gaatccgacc gcatcctgca
      421 acgcatcgaa gaaattgttc gttcgcagcc agaagtcgaa agcacttcgc gccgtaccgg
      481 tttacagctt ggccttgcgg cggtcacgga agccaatcgc ggcgatttct ccgtcaagct
      541 caagagcgat cggaagcgcg acatcgacga gatcatctcc gacattcgtt cggagatcga
      601 gcagaccgag cctgccgcaa aagtggaatt catccaggtc ttgcaggaca tgatcgggga
      661 cctcaccagc cagccgcagc ccatcgtcat taaacttttc gcgcaggatc ccaaactcct
      721 cgcgaatacc gctccgcacg tcgccgacgc cattcagaaa attcacggcg tcgtcgacgt
      781 tctcgacggt atcgagaaca ccatcagcgg acccgcaacc acttttcaaa tcaatcccgc
      841 cgtagctgcc cgcgcgggct tcacgcccga agagatcgcc gtagatgccg ccgccattct
      901 ggaaggcgaa cctgccagca caccggtggt tgtaaatgct cgcgcctaca cgatccgcgt
      961 tcgattcccg gaacaggccc gcgcaactct ggatcgcatg agcaacacgc tcctcactag
     1021 cgccaccggg cgcacggcaa ctctcggctc tctcgcaacg gtcagctcgg accccggaca
     1081 gacggaaatc cgccgcgaga atcttcagag gctcgtggaa gtcactgggc gcttcgaagg
     1141 tgtggacctc ggctcgggca tcaccgccgt caaaaaaatt gtcaacggcc tgcacctgcc
     1201 gtcctcgatc cgcgtggaat atggcggcac ttacgcggaa cagaggaagt ccttccgcga
     1261 cctcctgatg gtgctcgtgc tggcgctggt tttgctgttc gccgtgctct tgttcgaatt
     1321 ccgtacgttt tccgcgccaa ccgcgattct ggcttcggcc ttgctctcga ccttcggcgg
     1381 cttgctcgcc cttcttgtaa ctcgcacgac gtttaacgtc gcctcgttca tgggcatgat
     1441 catggtcatc ggcatcgtcg cgaaaaatgg cattcttctc ctcgacgccg aacatcgctt
     1501 ccgcgacctc ggcttttcgg cggaagaatc catgattcag gccggccgcc gccgtttgcg
     1561 gcccatcgtc atgaccgcgc tcgccaccgt cgccggcatg ctcccattgg ctttcgccat
     1621 tggaaaaggc tcccagatgc ttaagccgct ggccatcact gtggtcggcg ggatcctgag
     1681 ctccatggtg ctctcgctta tcttcacgcc cgccatccat ttctatctcc agccgaaaac
     1741 gcccgccgaa gcttgatttc aattcgcccc ccggtcctcc ccctccgtca tttcgaacgg
     1801 agcaggccga ttttttcttc ccgcttcgct cttgcgaaac cgcctctcca cccgtcattt
     1861 cgaacggagc aggccgattt tttcttccgc tttcgctcct gcgaaagcgt cggcctgcgc
     1921 agagagaaat ctctctgcgc ctttgtccga atcctcctct ctcttcacct accgctcgcg
     1981 actccgtccc cgacatcgtt aacgggctcg attcggatgc cgacgattat gtgacgaagc
     2041 cattttcctt tgcagtattg ctcgcccata tccgagcgct ggtgcgccgg gtacccgcta
     2101 cgttctggtt gcctcaagag cgacaaacca ccctgggtat gctctgcgct cggtatcgga
     2161 aatgatatcc tcctgccgtg cccaaaaaac ggcaagaaac agcaaacagg aaggaatcac
     2221 aaggcacaag acccgttctg tccttaccgg agtttgttgc tcgatggaaa gccagcaccc
     2281 tcaccgaacg ttccgcagcg caatcgcatt ttatcgacct ctgcgaagtc ctaggccagc
     2341 agcatccagc cgctgcggac cagactggcg agagctacac cttcgaaaag cacgtttcaa
     2401 aattgagagg cggtaaagga ttcaccgacg tatggaagcg cggccacttc ggatgggagt
     2461 acaaaggcaa gcatcatgac ttgcaagcag catacattca gctcgctgat tacagggaag
     2521 acctggaaaa cccgcctctg ttagtggtct gcgaccaaaa ccagtttgag gtacatacca
     2581 acttcactgg cactgcaaaa cgggtataca gatttactct tgatgagttg ctttcagatt
     2641 tgccgacggc gaattgcgcc ctgcccccgt tagaggttct ccgcgccctc tttaccaatc
     2701 cagatgagtt gaggccagag aaggcggcaa ctcggg
//
LOCUS       DASYVO010000425         7793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_7126, whole genome shotgun sequence.
ACCESSION   DASYVO010000425 DASYVO010000000
VERSION     DASYVO010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7126"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(463..708)
                     /locus_tag="VGR03_14290"
     CDS             complement(463..708)
                     /locus_tag="VGR03_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995496.1"
                     /translation="MARGWESKDVESQVEATQAPKHKFGAEPKTPEQLQREEQRKDLQ
                     LSRIRIANDLVSATHPNHRKSLEAALAHLDKKIADLS"
     gene            complement(776..1420)
                     /locus_tag="VGR03_14295"
     CDS             complement(776..1420)
                     /locus_tag="VGR03_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995497.1"
                     /translation="MSAASNPALSQPESFGKKFRHLAVGALFALVLLIPKILHVRRNP
                     RSWMLFRIFLGVAGAALVILPLGLGTSFVPAIVGLAMFISAILLPPAKPDTSADDKAH
                     ELGALVVVNGGCFQLGSAPSAAVQLFVGAKSIWVLDRRFQPLLEIHVSEITAACAEES
                     EESWLLRITWAGHAVDFSYRAIFAEHLARVAETTIRSVMRPALLVLPQRRAAGA"
     gene            complement(1445..1795)
                     /locus_tag="VGR03_14300"
     CDS             complement(1445..1795)
                     /locus_tag="VGR03_14300"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV1995498.1"
                     /translation="MADRLEDFQKFRADLNEEILGCGHLGIKCFFALDHQAYEPGPLG
                     TKNKELLGLVASAVLRCDDCITYHLVRCGEEGWKREEVIDALNVALIVGGSITIPHVR
                     RAFARMREIGGLQL"
     gene            complement(1866..3572)
                     /locus_tag="VGR03_14305"
     CDS             complement(1866..3572)
                     /locus_tag="VGR03_14305"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEV1995499.1"
                     /translation="MTIGNFQRWKSVFLSVVLLFGVVPLEAREPAQSFDIVITNGRII
                     DGTGSPWYSGDIGIRNGKIAFIGNLSSAPRARTIDARGNVVAPGFIDMLGQSEYTILV
                     DPRLPSKIYQGITTEITGEGESVAPLNDAILRADQEQYDHYHIQADWRTFREYFSRLE
                     KQRIGINIASYVGATRVRRMVLGDIDVQPTPAQLEQMKEFVSQAMRDGAVGVSTSLEY
                     APAPYAKTEELIALAGEASRFGGIYATHMRNEGTGILLAIDEALRIGHEAHIPVEIWH
                     LKVGGKPSWGRMPEVVAKINAARAQGLDVTADTYAYPAWFNDFSAFIPAWAHDGGNAK
                     LIERLKDPATRDRIRKDMLTPSDQWDNEWQEIPGPEAVLIGVVHNPQLLPLQGKTLAE
                     IAKSWNKDPMDALFDLLIEDHALTSVAVFGMSEPDVALALQQPWVSVDNDSSGTSPEG
                     ILGQEHPHPRAYGTFPRILRKYVREEHKLTLEDAIRKFSALPAQRMRLTDRGVLKAGM
                     CADIVIFDPARVHDVATFEIPNQLSQGMEYVLVNGVPVIDHSEMTGALPGKVLRGQGY
                     VP"
     gene            complement(3606..5153)
                     /locus_tag="VGR03_14310"
     CDS             complement(3606..5153)
                     /locus_tag="VGR03_14310"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1995500.1"
                     /translation="MEPSRRNFIKFVVVGSVAAGCPIDETLLAAPESKSPAAPLVHGE
                     HFEVCHQVRDGHQFERPDVTRKADIVIVGGGVAGLSAAYFLRGKDWLLLEKEPQFGGN
                     ALQEEFEDQIYGTGSAYAYHGDEGDQLASEIGLKMPFVNMPDPTIVNKTYVPDTWKTG
                     IDQLPYPKDVISSFKKFRDDIMKIKIREQMAELDAQHFTNFTAAYAPEVQQWWDGFGS
                     SNWGATTEDSSAYVGIANAQDLISGGDAKRAILPGGLGCITHKLVEVLQPKYKERMLD
                     NVTVVAAVQDKDAVRVTYLHEGKLITVSAKAVLMCSPKHITSRIVLGLPSEQKTAMRR
                     YRYAPYPVVNVIFDKPIYNRGYDNWCPGSAFTDFIVADWTVQKNPGYKQKHNILSFYT
                     PLRELQRSTLLDEDDCKSLAARVLADFQKLLPEFNVDPVEVRIYRRGHPMFMAVPGQH
                     TKNRLVASHPMDRIFFGNSDSGGPESLTSEAVRLSKAGSEWAELLLAGKPGARDLAEK
                     ALQAVPE"
     gene            complement(5162..5983)
                     /locus_tag="VGR03_14315"
     CDS             complement(5162..5983)
                     /locus_tag="VGR03_14315"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1995501.1"
                     /translation="MSAHAPVRRAPLLTIYAALVFIFIYLPVVVLILYSFNRDGVGGF
                     PPRHLTLDWYRQLFADAPIWDAVVNSLIVAAGSVVLSLTLGLLAALALDRATFPGKAL
                     FRRLVLLPLILPGIITGLSLLMFAVFAGAQLSLLTVFLGHGTALISVATTELFAGLQK
                     MDRAQEEASLDLGATPWQTFWRITLPNLKLSLIAAGLLIFTLSMDEIAVTFFLIGRDN
                     TLPLEIWGRLRRGITPEINAVSTLIFAVSVGLIVIWYRVRTRGLGTEAQELLERP"
     gene            complement(5980..6825)
                     /locus_tag="VGR03_14320"
     CDS             complement(5980..6825)
                     /locus_tag="VGR03_14320"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1995502.1"
                     /translation="MYSRAYKTLVSLPPLLWVGLFLLLPYALMFAHSFWAVHDGVLVH
                     QWNLHNFVTLFSKPVYVGVLLRTMRIAASVTLCSLLLGYPLAYYLSFHAGARKELLYQ
                     LVIVPLWMSYLVRGYAWKTILGSEGVLNGFLQYLHLTHEPVAFLLYSPFAVVLMLTHI
                     YTPFVFLPIYAALEHIPRPLVEASQDLGASSAQTFLRVILPLSIPGLLAGATFAFVLS
                     LGDFLAPLLVGGASGTMIANVVQSLFGAAYDWPLGAAISVCILITIISLLFLSERLEK
                     RWSFK"
     gene            complement(6844..>7793)
                     /locus_tag="VGR03_14325"
     CDS             complement(6844..>7793)
                     /locus_tag="VGR03_14325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1995503.1"
                     /translation="GEFLTLLGESGSGKTTLLRLVAGFEEPTAGEIWMSGQRLDTLPP
                     YKRRVNTVFQHYALFPHLSVRENVAYGLRVKKTSATEIDGRVDEALRMVKMQEFAASR
                     PSKLSGGQQQRVALARALVNRPLLLLLDEPLSALDANLRKQMQSELKALQRELGITFL
                     FVTHDQEEAMALSDRIALLRNGALEQVASPREIYAHPATAYTAQFIGQTNLLRSEILD
                     GTARCGALQWPADGPSGPAIFSLRPEAIRLASDAVAPPGTTRFRATLQQQFFSGASEL
                     LEVDCGGGQLLRVRISARGTLSGEREFVFSTADAIRVQE"
BASE COUNT         1656 a         2004 c         2611 g         1522 t
ORIGIN      
        1 tgctctccgc gatcgcatcg acgcgggtta tgaatacact gtctgcggtc gttgcttctt
       61 ttgagcttgc gctcaccttg tgtgccggcg cagatgctgt tgtattcata aatacacctc
      121 ccagtagatc aaacggcggc aacgtacggg cgcctcaatg gaagagtgta ttctattagg
      181 ggatctggga cgagggactc gttgtactcg tcccagatga ttagttttac gcgaagacct
      241 ttcgttcggg tttctaagat ttcatcaaat ggccatgcgc actcgttatc cactcgcata
      301 tcgcatcggc cggttccaga tctatggtcg cgcaatcggg aacaatggtt cgcaactatt
      361 cgcccgtgaa ttcggcttac cggaagatca ggccaaattg aaccgctggc acgctttgcg
      421 ctccgcgagg ttcgatgaga accagcaaca ttttccgaga ggctaactca aatcggcgat
      481 ttttttgtcg aggtgggcga gggcggcttc gagggatttg cgatggttgg gatgagtggc
      541 ggaaacgagg tcgttggcga tgcggatgcg cgagagttgc agatccttgc gctgttcttc
      601 acgctggagt tgttccggag ttttaggctc cgcgccaaat ttatgctttg gggcttgcgt
      661 cgcttccact tgtgattcga cgtccttgct ttcccagcct ctcgccatga atacagtgtg
      721 acacataatg tgaaaatcgc gggcgaatca gaagagaaaa gtggcgagaa gtatatcagg
      781 cgccagcggc gcggcgttga ggaagaacga ggagggcagg gcgcatcacg ctgcggatgg
      841 tggtttcggc gacgcgtgcg agatgctccg caaagatagc gcggtaggag aaatcaacgg
      901 cgtgaccggc ccaagtgatt cgcagaagcc aggattcttc ggattcctcg gcgcaggcgg
      961 cagtgatttc gctgacgtgg atctcgagga gcggctggaa gcgccggtcg agcacccaaa
     1021 tacttttggc gccgacgaag agctgcacgg cagcagacgg cgcacttccg agttgaaagc
     1081 accctccatt aacgacaacg agagctccca gctcgtgggc tttgtcatcg gcgctggtat
     1141 cgggtttggc gggtggcaga agaatggcgc tgatgaacat ggccagaccg acgatggccg
     1201 gaacgaagct ggtccccaag ccgagcggca ggatgaccag cgccgctccg gcaactccca
     1261 ggaaaatgcg gaagagcatc caagaacgcg ggttgcgccg cacgtgcagg atctttggaa
     1321 taagcaagac caaggcgaac agagctccga cggcgaggtg gcgaaacttt ttgccgaaac
     1381 tctccggttg ggaaagcgcg gggttggacg cggcgctcat ctttgatctt tcaaggctga
     1441 tctctcaaag ttgcaggccg ccgatttcgc gcatacgcgc aaaggcgcgc cgcacgtgag
     1501 gaatggtgat cgagccgcca acgatcagcg ccacgttgag cgcgtcgatc acttcttcgc
     1561 gcttccagcc ttcctcgccg cagcggacca aatgataagt gatgcagtca tcgcagcgca
     1621 gcaccgcgga ggcgaccagc ccaagtaatt ccttgttctt ggtgccgagc gggccaggtt
     1681 cgtaggcctg gtggtcgaga gcgaagaagc atttgatgcc gaggtggccg cagccgagga
     1741 tttcctcgtt caggtcggcg cgaaacttct gaaaatcttc caggcgatcg gccatggttc
     1801 ctccgggggg acaaatctca acgcaccaac cgtgcggcca ttatgaacca actgcgccca
     1861 tcctgttagg gcacgtaacc ctggccgcgg agcactttgc cgggtaacgc gccagtcatc
     1921 tcgctatggt cgatgaccgg aactccgttg acgagcacgt attccattcc ctgcgacagt
     1981 tggttaggaa tctcgaaggt ggcgacgtcg tgaactctcg ccggatcgaa gatgacgatg
     2041 tcggcgcaca tccccgcctt caagacgcca cggtccgtca agcgcatgcg ttgggcgggc
     2101 aatgcggaga attttcggat agcgtcttcg agggtgagct tgtgttcttc acggacgtac
     2161 ttgcgcagga tgcgagggaa cgtgccgtat gcgcgcggat gaggatgttc ctgcccgagg
     2221 atcccttcgg gcgaagtgcc ggaggagtcg ttgtccacgg agacccaggg ctgctggagc
     2281 gcgagggcaa cgtccggctc ggacatgccg aagacggcaa cggaagtgag tgcatggtct
     2341 tcgatcagga ggtcgaagag ggcgtccatc ggatccttgt tccaactttt tgctatttcg
     2401 gccaaagtct tgccttgcag cggaagcaat tggggattgt gcacgacgcc gatgaggacc
     2461 gcttcggggc cgggaatctc ctgccattcg ttgtcccatt ggtcggaagg tgtgagcata
     2521 tccttgcgga tgcgatcgcg cgttgcggga tccttcaaac gttcgatgag cttggcgttg
     2581 ccgccgtcgt gcgcccaggc aggaatgaaa gcggagaaat cgttgaacca tgcggggtag
     2641 gcgtaagtgt ccgcggtgac atcgagacct tgcgctcggg cggcgttgat ctttgcaacg
     2701 acttccggca tgcgccccca gcttggcttg ccgccaactt tcagatgcca gatttcgacg
     2761 gggatatgag cttcgtggcc gatgcgcagg gcttcgtcga tggccagtaa gataccggtg
     2821 ccttcattgc gcatgtgcgt ggcgtagatg ccgccgaacc tggaggcttc cccggcgagg
     2881 gcgatcaact cctcggtttt ggcgtaggga gcgggagcgt attcgagaga agtcgagacg
     2941 ccgacagcgc cgtcgcgcat cgcctggctc acaaactcct tcatttgctc gagctgcgcg
     3001 ggcgtgggct gcacgtcgat atcgccgagc accatacgcc ggacgcgcgt ggcgcctacg
     3061 tagctggcaa tgttgatgcc gatgcgttgc ttttcgagcc gggaaaaata ttcgcgaaac
     3121 gtccgccagt cggcttgaat gtgatagtga tcgtattgct cctgatcggc gcggagaatg
     3181 gcgtcgttca gcggggcgac ggattcgccc tcgccggtga tttccgtggt gattccctgg
     3241 tagatcttgg agggtaggcg ggggtcgaca agaatggtgt actcggactg gccgagcatg
     3301 tcgatgaaac cgggagcgac aacgttgccg cgtgcatcga tggtgcgagc gcgcggtgcg
     3361 gagctaagat tgccgatgaa ggcaatcttt ccgttgcgaa ttccgatatc gcccgaatac
     3421 cagggcgagc cggtgccgtc gatgatgcgg ccgttggtga tgacgatgtc gaaagactgc
     3481 gccggttccc gggcctcgag aggaacgacg ccgaagagca agaccacgga taagaacacg
     3541 gacttccagc gctgaaaatt accgatggtc ataagttgat cctcgctccg cgcacagcgg
     3601 gcatcttact ctggaactgc ttgcaaggct ttctccgcta agtctcgtgc gccgggtttt
     3661 ccggcgagga ggagttccgc ccactccgag ccggctttcg agagacggac ggcttcgctg
     3721 gtgagcgatt cggggccgcc ggaatcagag ttgccgaaaa agatgcggtc catcggatgg
     3781 gaagcgacga ggcgattctt cgtgtgctgg cccggcacgg ccatgaacat cggatggccg
     3841 cggcggtaga tgcgtacttc cacggggtcg acgttgaatt cgggcaacag tttttggaaa
     3901 tcggcgagga cgcgagcggc gagggacttg cagtcgtcct cgtcgagcag cgtggagcgc
     3961 tggagttcgc ggagcggcgt gtagaacgac aggatgttgt gtttttgctt gtagccggga
     4021 ttcttctgga cggtccagtc ggcgacgatg aaatcggtga aggcgcttcc ggggcaccag
     4081 ttgtcatagc cgcgattgta gatcggtttg tcgaagatga cgttgacgac ggggtatggc
     4141 gcgtagcggt agcggcgcat ggcggttttc tgttcagagg ggagacccag gacgatgcgc
     4201 gaggtgatgt gctttggaga gcacatcaga acagccttag cggagacggt gatgagtttt
     4261 ccttcgtgga ggtaggtgac gcggacggcg tctttgtctt ggacggcggc gacgacggtg
     4321 acattgtcga gcatgcgctc tttgtatttc ggctggagga cttcgacgag tttgtgggtg
     4381 atgcagccga gaccgccggg aagaatggcg cgcttggcgt cgccgccgct gatgagatcc
     4441 tgagcgttgg cgatgccgac gtaggcggag ctgtcttccg tggtcgcgcc ccaatttgag
     4501 gagccaaaac cgtcccacca ttgttggact tccggagcgt aggcagcggt gaaatttgtg
     4561 aagtgctgcg catcgagctc ggccatttgt tcgcgaatct ttattttcat gatgtcatcg
     4621 cgaaatttct tgaagctgga gatgacgtct ttgggataag gcagctggtc gatgccggtc
     4681 ttccaggtgt cggggacgta cgtcttgttg acgatggtgg gatcgggcat gttgacaaaa
     4741 ggcattttga ggccgatttc ggaggctagc tggtcgcctt catcgccgtg ataggcgtag
     4801 gcggagccgg tgccataaat ctggtcttcg aattcctcct gaagagcgtt gccgccgaac
     4861 tgaggttctt tttcgaggag gagccaatct ttcccgcgga ggaaataagc cgcggacagg
     4921 cccgcgacac ctcctcccac gatgacgata tcggcttttc gtgtgacgtc ggggcgttcg
     4981 aactggtggc cgtcgcgaac ctggtggcaa acttcgaaat gttctccgtg aacgaggggc
     5041 gcagcaggcg atttcgattc cggcgcggcg agaagcgttt catcgatggg acagccggcg
     5101 gcgacggaac caacgacgac gaatttgatg aaattgcggc gtgacggctc catacaacct
     5161 cctagggtct ttcgagaagt tcctgggctt cagtgccgag gccgcgggta cgcacgcgat
     5221 accagatgac gatgaggccg acagagacgg cgaagatcag agtggagaca gcattgattt
     5281 cgggagtgat gccgcggcgg aggcggcccc agatttcgag cggcaaagta ttgtctcggc
     5341 cgataaggaa aaaagtgacg gcgatctcat ccatggagag cgtaaagatg agcaggccgg
     5401 cggcaatcag agagagcttg agattaggaa gagtgatacg ccagaaggtc tgccacggcg
     5461 tcgcgccgag atcgagggaa gcttcttcct gagcgcggtc cattttttga aggccggcaa
     5521 agagctcggt ggtggcgaca gagatgagcg cggtgccatg gccgagaaaa acggtgagca
     5581 ggctaagctg ggcgccggca aaaacggcga acatgagcag ggaaagaccg gtgatgatgc
     5641 cgggaagaat cagcggcaac agaacgaggc gtcggaagag cgcttttccg gggaaggtgg
     5701 cgcgatcgag tgccagcgcg gccagcagcc ccagagtcag tgaaagcacg acgcttcccg
     5761 cggcgacgat caggctgttc acgacggcat cccagatggg ggcatcggcg aaaagctggc
     5821 ggtaccaatc gagcgtgagg tggcggggcg ggaaaccgcc gacgccatcg cgattgaagg
     5881 agtaaagaat gagaacgacg accggcagat agataaagat gaaaacgagc gcggcataaa
     5941 tggtaagcag cggagcgcgg cggactggag cgtgggcgct catttgaagg accaccgctt
     6001 ttcgagccgc tcgctgagga agagcaggga gatgatggtg atcaggatgc aaacagaaat
     6061 tgccgctccg agcggccaat catacgccgc accgaagagg ctctgcacga cgttggcgat
     6121 catcgtgccg gaagcgccgc caacaagcag cggcgcaagg aagtcgccga gcgagagaac
     6181 gaaggcgaat gttgcgcccg caagcaagcc gggaatggaa agaggcagga tgacacgcag
     6241 aaatgtttga gccgagctgg cgccgaggtc ctgcgaggct tcgacaagcg ggcgagggat
     6301 gtgctcgagg gctgcgtaaa tgggcagaaa aacgaaaggc gtgtagatgt gcgtcagcat
     6361 gagaacgacg gcaaaagggc tgtagagaag gaacgcgacg ggctcgtggg tgagatgcaa
     6421 gtattgcaag aagccattga gtacgccttc gctgccgaga atggttttcc acgcatagcc
     6481 gcgcaccagg tagctcatcc acagcggcac gatgacgagc tggtagagca gctctttgcg
     6541 ggcaccggca tgaaacgaca ggtaataggc cagcgggtag ccgagcagca gggagcacag
     6601 cgtcaccgaa gcggcgatgc gcatggtgcg caggaggact ccaacgtaga cgggcttgga
     6661 aaagagggtc acaaaattgt gcaggttcca ctggtggacc agaactccgt catggacggc
     6721 ccagaagctg tgcgcgaaca tcagtgcgta aggaagcagg agaaacaagc cgacccagag
     6781 gagcggcggg agagagacga gcgttttgta cgcgcgcgag tacatgggca gggataagag
     6841 cgattactcc tggacgcgga tggcgtctgc ggtcgaaaaa acaaattcgc gttcgccgga
     6901 aagcgttcct cgcgcggaaa tacgcacgcg caggagctgg ccgccgccac aatccacttc
     6961 gagcaattca ctcgcgccac tgaagaactg ctgctgaagc gtggcgcgga accgcgtggt
     7021 tcccggagga gccacagcat cggaggcgag acgaatggcc tccggacgca acgagaaaat
     7081 ggcgggaccg cttgggccat ccgcgggcca ctgcagggcc ccacatctgg ccgtgccatc
     7141 cagaatttcg ctgcgcaata aattggtctg gccgatgaat tgggcggtgt atgccgtggc
     7201 gggatgcgcg tagatctcgc ggggagaggc gacttgctcc aacgcgccat tgcgcagcag
     7261 cgcaatgcgg tcggagagcg ccatggcttc ttcctggtcg tgggtgacaa aaagaaaagt
     7321 gatgccgagc tcgcgctgca gggctttcaa ttcgctttgc atctgcttgc gcagattggc
     7381 gtcgagcgcg gaaagcggct cgtcgagcag gagaagcaga ggacgattga cgagcgcgcg
     7441 cgcgagggcc acgcgttgtt gctggccgcc gctcagtttg gacggacggg aagccgcgaa
     7501 ttcctgcatc ttcaccatgc gcagagcttc atcgacgcgg ccgtcgatct ccgttgcgga
     7561 agttttcttg acgcgcaagc cgtaggcgac gttttcccgc acactcagat gcgggaataa
     7621 cgcgtagtgc tggaacacgg tgttcacgcg gcgcttgtaa ggtggaagcg tgtccagccg
     7681 ctggccgctc atccagattt ctcccgcggt tggttcttcg aatccggcga ccagacgaag
     7741 aagagtagtt ttgcctgaac cgctttcgcc gagcagggtg aggaattctc cgg
//
LOCUS       DASYVO010000426         5342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_207368, whole genome shotgun sequence.
ACCESSION   DASYVO010000426 DASYVO010000000
VERSION     DASYVO010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207368"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VGR03_14330"
     CDS             complement(<1..693)
                     /locus_tag="VGR03_14330"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HEV1995504.1"
                     /translation="MSAGTPPLSGPPKPQAKALNCPKCGAAITLRSFGQAETVVCSSC
                     HSILDAKDPNLAILQQFEAATREVKPLIPLGTRGKLRGTDYEVIGFQRRSTTVDGITY
                     YWHEYVLFNPYKGIRYLSEYNGHWNDISICKTLPTADIGGVTTANYLGESYKHFQSAD
                     ANTDFVLGEFPWQVRVGEHAAVTDFVHPPRVLSSEKLDKEVTWSIGEYMYGLEIWDAF
                     KLPGAPPEAIGVY"
     gene            complement(693..1325)
                     /locus_tag="VGR03_14335"
     CDS             complement(693..1325)
                     /locus_tag="VGR03_14335"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HEV1995505.1"
                     /translation="MTQPVANCPNCGAKIVFRWSSSVQTVCEFCKSILVRTDVDLKKV
                     GQVADLPPDSSPIRINTEGKYGNKAFVVVGRILYEYDQGAWSEWHVMMNDGTSAWLSD
                     AQSEYALSLSSKAQNLPAVALVQVGQQFTWSNQRYTVSVITPAHYRGVEGELPFQYWD
                     KTDVTFVDLRTESRKFATLDYSDPEPALYLGEFVEFDDLKMKNLRSFEGW"
     gene            complement(1365..1736)
                     /locus_tag="VGR03_14340"
     CDS             complement(1365..1736)
                     /locus_tag="VGR03_14340"
                     /inference="COORDINATES: protein motif:HMM:NF014705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HEV1995506.1"
                     /translation="MSGIEWVVEAFGCSSQSLQDTAVLQDLFNSIIREMKLRPVGETH
                     WHQFPGTGGITGLCLLAESHLACHTFPEHGSLCLNLFCCVPRAEWDFETVLRQLFDAH
                     SVTVRRLRRPYESKLKQVASD"
     gene            complement(1751..2749)
                     /locus_tag="VGR03_14345"
     CDS             complement(1751..2749)
                     /locus_tag="VGR03_14345"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1995507.1"
                     /translation="MSLRDFISKQFIDVIEWVEPEEGILAYRYPMQDREIQNGGKLTV
                     RDSQAAVFVNEGKVADIFGPGLYTLNTQTLPILTYLRNWDKAFKSPFKSDVYFFSARI
                     QTDQHWGTQNPISIRDKEFGAIRLRGFGIYSYHLSDAKSFYSKISGTRDLYRVADLEG
                     QLRNTIIAKMTDAFAASQVPFLDMAANQGALAEKISETLKPSFAEYGLTLDTLVVENL
                     SLPDELQKVLDQRISMNVLGDMGKFTQYQVAQAIPIAAANEGGGAVGMGAGLGAGVAM
                     GQAMMDAVKKSTSSPAGGAAPAAAGAPAAETKFCSECGKPIARNAKFCPECGKPQV"
     gene            complement(2979..>5342)
                     /locus_tag="VGR03_14350"
     CDS             complement(2979..>5342)
                     /locus_tag="VGR03_14350"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEV1995508.1"
                     /translation="GSSDAAALVGMLASRALHGAEEFTARPTLPLYLPGEPVEVELRW
                     NSAEQPAAPLTVRLTMFPEALPEQRSVVTATVPSSQTAIISPPKEKGFHVIEAELLKG
                     GKVHAIYRSGFWIRDEDFLRSGPRLTANRDYLELDGHPLAVVGTTYMSSEVQRLFFDH
                     PNAYIWDRDIAQIKAAGLNTIRTGWWTGWDKLCDENGRPYERTMRTLEAYLMTVRKHG
                     IPVQFNFFAFLPEVLGGANPYLDPQAIRKQQTLVSSVVARFHDAPFLAWDLINEPSIS
                     KHLWRMRPNGDPIELAAWNQWLSKRYPDHAALAAAWGVLPASVEGTISLPDEIEFAPR
                     GMYVGHNSLRVFDYYLFAQDTFAGWARAMHEKIRATGSQQLITVGQDEGGVMDRLSPA
                     FFAPALDFTTNHSWWQNDSLLWDSLTAKQPGEAMLIQETGLQRELNLNETARLTPEQE
                     GAIFERKVAMSFVQGSGAIEWLWNTNSYMTEANESPIGALRTDGSEKPEATVMRGFAR
                     FAQSAQDHLRNPQQPSVAIVASQAAQFSILGDLQLEAQRKAVRALAYGLHIAPYVIAE
                     NQIAKLGSPRLVILPSPQALCESTWQALLRYVKDGGNLLITGPVSRDEHWQWRDREHD
                     LGLNAHIEPQNYRGAAIRLPDKAISLSYDQQKQFALEALRFDDGATWKETASGKGKIF
                     WSSYPAELAEGLDPAVALYGYVSAALKIKPMFELQSSLPPSVLVYSTELQDSLLYILE
                     SETTDDTQIDIRDTLTGVRLTLKLPAQRAAIALIGKHEKAVLAQYGF"
BASE COUNT         1112 a         1452 c         1644 g         1134 t
ORIGIN      
        1 gtagacgcct atcgcttcgg ggggagcgcc gggcagcttg aaggcgtccc agatttccag
       61 gccgtacatg tactcaccga tggaccaggt gacttccttg tcgagctttt cgctggaaag
      121 gacgcgcggc ggatggacga agtccgtgac agcggcgtgt tcgccaacgc gaacctgcca
      181 cggaaattca ccgaggacga aatccgtgtt ggcatcggcg gactggaagt gcttgtaact
      241 ttccccgaga taatttgccg tcgttactcc gccaatatcc gcggtaggca gcgtcttgca
      301 aatactgatg tcgttccaat gtccgttgta ttcgctgagg tagcgaatgc ccttgtaagg
      361 attaaacaga acgtattcat gccagtagta ggtgattcca tcgaccgtgg tgctgcgccg
      421 ctggaacccg atgacttcgt agtccgtgcc gcgcagcttg ccgcgcgtgc cgagaggaat
      481 gagcggtttg acttcacgtg tggcggcttc gaattgctga agaatcgcga ggttgggatc
      541 tttggcgtcg agaatcgaat ggcaactact gcacacgacc gtttcagcct ggccgaacga
      601 acgcagcgtg atggccgcgc cgcacttcgg acaattcagc gccttcgctt gcggcttcgg
      661 aggcccggaa agtggtggag tacccgcgct catcaccagc cctcgaagct gcgtagattt
      721 ttcattttca gatcgtcgaa ttccacgaat tcaccgagat acaaagccgg ttcgggatcg
      781 ctgtaatcga gcgtagcgaa cttcctggac tccgtgcgca gatcgacgaa ggtgacgtcg
      841 gtcttgtccc aatactgaaa cggcaattcg ccttcaacgc cgcggtagtg cgctggagtg
      901 atgacgctca cggtatagcg ctggttgctc caggtgaatt gctgacccac ctggaccagc
      961 gcaacggcgg gaaggttctg tgctttcgaa gacaaagaca gcgcgtattc actttgcgca
     1021 tcggagagcc aagcgcttgt gccgtcgttc atcatgacgt gccactcgct ccaggcgcct
     1081 tgatcgtact cgtaaaggat gcggccaact acaacgaaag ccttgttgcc atacttgcct
     1141 tcggtattga tccgaatggg tgagctgtcc ggcgggagat cggcgacctg gccgactttt
     1201 ttcaggtcca catccgtgcg gacgaggatg ctcttgcaaa attcgcacac ggtctgcacg
     1261 ctgctggacc agcgaaaaac gatcttggcc ccgcagttcg gacagttggc aacgggctgg
     1321 gtcatcgcga atgtcccacc ggccttgcgg caagaggttt tacctcaatc actggcgacc
     1381 tgcttcaatt tggattcgta gggcctccgc agccgacgga ccgtaacgga atgcgcatcg
     1441 aagagttgcc gcagaacagt ttcgaagtcc cactcagctc gcggaacgca gcagaagaga
     1501 ttgagacaga gcgagccgtg ctccggaaac gtgtgacacg cgaggtgcga ctccgcgaga
     1561 agacacaggc cggtgatacc tccggtgccg ggaaactgat gccaatgcgt ttcgccgacg
     1621 ggccggagct tcatctctcg gatgatgctg ttgaacaaat cctgcaagac agcagtgtcc
     1681 tgcagcgact gcgacgagca tccgaaggct tcaacaaccc actcgattcc gctcatgaca
     1741 ggcgttagca ctacacctgg ggctttccgc attccggaca gaatttagcg ttgcgcgcga
     1801 tgggcttgcc gcattcactg cagaacttgg tttcggcagc gggagctccg gccgccgcgg
     1861 gtgccgctcc accagcgggc gacgatgtcg atttcttcac cgcgtccatc atggcctggc
     1921 ccatggccac gcccgcgccg agacctgcgc ccattcctac cgcgcctccg ccttcattcg
     1981 cggccgcgat ggggatggcc tgcgccacct gatattgcgt gaacttgccc atgtcaccga
     2041 gcacgttcat gctgatgcgc tggtcgagca ctttctgcaa ttcgtccgga agcgaaaggt
     2101 tttcgaccac caaggtgtcc agggtaagtc cgtattccgc gaaggacggt ttgagggtct
     2161 cgctgatttt ttcggcaagt gcgccttgat tcgcggccat atcaaggaag ggcacctggc
     2221 tggcggcgaa ggcatcggtc atcttggcaa tgatggtgtt gcgcagctgg ccttcgagat
     2281 ccgcgacgcg atagaggtcg cgagtgccgc tgatcttgct gtagaacgat ttggcatcgc
     2341 tcaagtgata ggagtaaatg ccgaagccgc gaagacggat ggcgccgaat tccttgtcgc
     2401 gaatggagat gggattctgt gtgccccagt gctggtcggt ctgaattcgc gccgaaaaga
     2461 aatagacatc ggacttgaac ggcgacttga acgctttgtc ccagttcctc aggtaagtca
     2521 ggatgggcag cgtttgcgta ttcagcgtgt agagtccagg gccgaagatg tccgcaactt
     2581 tgccttcatt tacaaaaact gcggcttgcg aatcacggac ggtcagcttg ccgccattct
     2641 ggatctcgcg atcctgcatg gggtagcggt aggccaggat gccttcttcc ggctcaaccc
     2701 attcgatgac atcgataaac tgtttggaga tgaagtcgcg aaggctcatg agaggcctct
     2761 cttccggcgc gctcgtgagc aagaatagct gccggtgaaa gcgatgctac ggtgcggctc
     2821 gcagtgtgtc aatggttcgc ataagaatgc aattgcgcga atatagctgg gcacggtcca
     2881 aataaagcac tctgcgctac ccccaggaaa tacaaggaga taggcagcac gccggattgc
     2941 agacgtatta caaaatcttt cgtgattctt gccacggctc aaaatccata ctgggcgagc
     3001 accgcttttt cgtgctttcc gatcagggcg atggcggcgc gttgtgccgg gagtttcagc
     3061 gtcaggcgaa caccggtgag tgtgtcgcga atgtcgatct gcgtatcgtc cgtggtttcc
     3121 gattccagaa tgtacagaag ggagtcctgt agttcggtgg aatagactag gacgctcggc
     3181 ggaagcgaag attgcaactc gaacatcggc ttgattttca acgcggctga aacgtagccg
     3241 tataacgcca ccgcgggatc gaggccctcg gccagttcag cgggatagga agaccagaag
     3301 atctttccct tgcccgaagc cgtttctttc caagtggcac cgtcgtcgaa gcgcagcgct
     3361 tccagggcaa attgcttttg ctggtcgtag gacaaagaga tggccttgtc cggcagacgg
     3421 atggccgcgc cgcgatagtt ctgcggttcg atatgagcgt tcaggccaag atcgtgctcg
     3481 cggtcccgcc attgccaatg ctcatcgcgg gaaaccggcc cggtgatcaa gagatttccg
     3541 ccgtctttca cgtatctcag aagggcctgc caggtgctct cgcacaaagc ttgaggggag
     3601 ggcagaatga cgagcctggg cgaaccgagc ttggcgatct gattttcagc gatgacataa
     3661 ggagcgatgt gcagaccgta ggcgagagcg cgcaccgctt tgcgctgcgc ttcgagttgc
     3721 aagtctccca ggatggagaa ttgcgctgcc tgcgacgcga cgatcgcgac ggaaggctgt
     3781 tggggattgc gcaaatgatc ctgcgcggat tgggcaaatc gcgcgaagcc acgcatcaca
     3841 gtggcctcgg gtttttcgga accgtccgtg cgcaatgcac cgatgggcga ttcgttagcc
     3901 tcggtcatgt aggaattggt attccatagc cattcgatcg cgccggagcc ttgcacaaaa
     3961 gacatggcaa cttttcgttc gaagattgcg ccttcctgct cgggagtgag gcgggccgtt
     4021 tcattgagat tcaactcgcg ctggagcccg gtttcctgaa tcaacatggc ttcgcccggc
     4081 tgcttggcgg tcagggaatc ccagagcaga gaatcgttct gccaccagga gtgattggtc
     4141 gtgaaatcga gagcgggtgc gaagaatgcc ggcgagagcc gatccatgac gccgccttca
     4201 tcctgcccga cagtgatgag ctgctgtgaa ccggtggccc ggattttttc gtgcatcgct
     4261 cgggcccaac ctgcgaaggt atcctgcgca aacaagtaat agtcgaagac gcgcagcgaa
     4321 ttgtgtccta cgtacatccc gcgaggcgcg aactcgattt cgtcgggaag tgaaatggtt
     4381 ccctcgacgg aagcaggcag aacaccccaa gccgcggcta acgccgcgtg atcgggatag
     4441 cgtttcgaga gccactggtt ccacgccgcg agttcgatgg ggtcgccgtt cggccgcatc
     4501 ctccataagt gttttgagat gctgggctcg ttaatgagat cccaagcgag gaatggagcg
     4561 tcgtggaatc gcgcgacaac cgaggacacg agcgtctgct gcttgcgaat cgcttgagga
     4621 tcgagataag gattcgcgcc gccgaggact tcggggagga atgcgaagaa attaaactgt
     4681 acagggatgc cgtgctttcg cacggtcatc aaataagcct cgagggtgcg catggtgcgc
     4741 tcataggggc gcccgttttc atcgcaaagt ttgtcccagc cggtccacca gccggtgcga
     4801 atggtgttga ggccggcggc tttgatctgg gcaatgtcgc ggtcccagat ataagcgttg
     4861 ggatgatcga agaagagccg ctgcacctcg ctggacatgt aggtagttcc cacaacagcg
     4921 agcgggtggc cgtcgagctc gaggtaatct cgattggcgg tcaagcgcgg accggagcgc
     4981 aggaagtcct cgtcgcgtat ccagaagccg gaccgataga tggcgtggac tttgccgccc
     5041 ttgagaagct cagcctcgat gacgtgaaat cctttttcct tgggcgggga gataattgcc
     5101 gtttgagatg agggaacggt tgccgtcacg acggaacgtt gttccgggag ggcctccgga
     5161 aacatggtga gtctcacggt cagcggcgcg gccggttgct cggcggaatt ccatcttaat
     5221 tcgacttcaa ccggctcacc agggagatag agcggaagcg tggggcgggc cgtgaactct
     5281 tcagcgccgt ggagcgcgcg actggcaagc ataccgacga gggcggctgc atcgctactg
     5341 cc
//
LOCUS       DASYVO010000427        12240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_278126, whole genome shotgun sequence.
ACCESSION   DASYVO010000427 DASYVO010000000
VERSION     DASYVO010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12240
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278126"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VGR03_14355"
     CDS             <1..770
                     /locus_tag="VGR03_14355"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEV1995509.1"
                     /translation="SPYLRNLQFDVEALSAPDARMEWPGAQLQADANLRVRGTWEHPI
                     LLGHIHILSGDLYFAGNRYRVTRGDLNFANPFRLDPVLNVEATTTIQQYEITLNFNGP
                     ASKLTLAYRSDPPLPANDIITLLALGQTSSEGTARSGGTSQSGTAGASAILSEAISSQ
                     LGGRLERLFGITRFRVDPGLAGVDSTGSEQNAAARITVEQQITPNLTITYVSNVSSTQ
                     QQVIQVEYNVDRNLSIVGLRDQNGTFGIDIKIKKRFR"
     gene            complement(850..1479)
                     /locus_tag="VGR03_14360"
     CDS             complement(850..1479)
                     /locus_tag="VGR03_14360"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEV1995510.1"
                     /translation="MKAVLALVIIYVGTFFLVIQGASQNSVQAAPQSATPVQGNAAPP
                     QANSSIDPAKEADIRSLMELVGARDLVQDGANTAIEQSREKLLATVPNNDKGQAFVNA
                     FAVSYQKKFDVDQVTDQLVGIYDKHFTEDEINALLQFYGSPLGQKVAEEMPKIGRETQ
                     AATRAATAKAAKDALAELKQQNPEIGQSARLGNGQGQGRWQQRRGQQRG"
     gene            1644..3080
                     /locus_tag="VGR03_14365"
     CDS             1644..3080
                     /locus_tag="VGR03_14365"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV1995511.1"
                     /translation="MNKTEMQGGASLGAPAPAGAIEREAGLQRNLTPGQLAMIGLGST
                     IGTGLFLGSAISVKLAGPAVILSFAGAACIAVTMMWALAEMAAAHPAAGSFGLYAEMY
                     LHPWAGFAVRLTYWFCMMVVVGSEVVAASIYSSFWFPTTPSWVWIGLFSLVILYVNTI
                     SIGKFGTFEYILSMFKVITIVVFLILGTALLTGVGFPKLGFANYTAHGGFLPNGWKGV
                     GLGVILGLFSFFGIEVVGSTAGEAADPKAAVPKALRRTLFALVLFYVAGLALVVGIVP
                     WQQIGLSESPFVRVFKTVGIPGASDIMNLAVLTAALSSAVANLYFGARLAFSLSRGGY
                     LPAILGRLSRKGMPVIAVLASAGGMAAALVLSEYFKDSLFVFMIGLSTFGALFAWLMT
                     LLTHLAFRRFHKRQARPYLQLGPPGPWASLMGLGGVLAVLVSTWWVPEFRITLLAGIP
                     WLGFITVCYFVWRRARSRMPIQRGAQDG"
     gene            3073..4257
                     /locus_tag="VGR03_14370"
     CDS             3073..4257
                     /locus_tag="VGR03_14370"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV1995512.1"
                     /translation="MDELLKWRGEFPILDRITYMISNSLGAMPRGVYDGLRSYADSWA
                     ERGVRAWEEGWWEMAVSVGDQIAPLIGAGPGEISLHQNVTLTQAVISSCFDFRGPRNK
                     VVMVELEFPSIQYFYHEQRRLGARVEVVPTPDDIRIDLDKLLAAIDETTLLVPISKVL
                     FRSSCIVDARAIIERAHRVGAHVILDVFQAAGSIPLDVRGLGVDFAVGGVLKWLCGGP
                     GVGYLYVREDLRAKLRPALTGWIAHRRPFAFETGAIDPREDSFRYLNGTPHIPALYAC
                     QPGLEILNKVGIAAIRDKSIRMTARLIESARSRGWRVNTPENPAERAGTVSVECPHAY
                     EVCRELLARDIIVDYRPKAGVRVSPHFYNREDECDFTLAQMEEILKTGAWEKHAAAVT
                     HA"
     gene            4250..4909
                     /locus_tag="VGR03_14375"
     CDS             4250..4909
                     /locus_tag="VGR03_14375"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEV1995513.1"
                     /translation="MPEKKEYAAQRVEMIEKQLRRRGISDAAVLGAMAAVPRHEFVAE
                     ELRSHAYDDLPMPIGGGQTISQPYIVAAMTAALHLQPGDRVLEIGTGCGYQAAVLSRL
                     AREVFTIERRPELASSASATLARLGYPNAHVHCGDGTLGLAEFAPFDAILVAAAAPAV
                     PKPLLAQLAEGGRMILPVGDAEHQELQLIEKRGDAFPTKLLEGCRFVPLVGYHGWREP
                     S"
     gene            4912..5652
                     /locus_tag="VGR03_14380"
     CDS             4912..5652
                     /locus_tag="VGR03_14380"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEV1995514.1"
                     /translation="MSSTAPDLSIIIPSYNEELRLPATLERIASYLSTYGREAEVLVV
                     DDGSKDGTAAVAEYFRIKIPTLRVVSNGVNRGKGYSVRHGMQEARGRIALFTDADLSA
                     PIEEAGKLLDALETCDVAIGSRAMDRSLISLHESPFREFAGIIFNKIVRIILWLPFVD
                     TQCGFKAFRRERCGILFEQQTIERFGFDPELLYLARRHGLHAIEIPVRWGHSPATKVS
                     MLHDSIQMFIDVFTIRWNSLMGRYPRKA"
     gene            5722..6336
                     /locus_tag="VGR03_14385"
     CDS             5722..6336
                     /locus_tag="VGR03_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995515.1"
                     /translation="MATSGPLRSPKREPIPIDARAADHLRYIRETMESAAEFTAVPGW
                     GGVAMGVTALVAAFAASRQTTPRAWLTVWLIEAFVAVAIAAPAAATKAHRANSALFSG
                     PGRKFVLSFAPPIVVGGLLTFALYNAGILAALPGVWLLLYGTAIVTGGAFSVRVVPVM
                     GLCLMMLGSAALFAPAVWGDAFMAAGFGVVQIGFGWWIARHYGG"
     gene            6348..6740
                     /locus_tag="VGR03_14390"
     CDS             6348..6740
                     /locus_tag="VGR03_14390"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1995516.1"
                     /translation="MAKSSTAHQERLQRSRERGASLEAIRNPARETVPADLDRLIHER
                     IRLGIVSALAVNRSLTFNELKALLKTTDGNLSVHARKLEEADYIVCTKSFDGRLPKTE
                     YRLAAAGRRALERYLNHMEALIRATREG"
     gene            6816..7574
                     /gene="uppS"
                     /locus_tag="VGR03_14395"
     CDS             6816..7574
                     /gene="uppS"
                     /locus_tag="VGR03_14395"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEV1995517.1"
                     /translation="MLMHHLTVRQIPTTQPAIAQATRLHVAIISDGNGRWATSRGLPR
                     SAGHRAGAESARRVIEAGPRIGIHTLTLFALSSANWKRPAAEVHAILRLLHEYLLTET
                     THCIDEGVRVSIIGRRDRLPATLRQAIADSEAATATGTRLHLRLAVDYSAREAIYHAA
                     CRFYKVTELSPESFSTVLAEVHRGGSTEVDLLIRTGGEQRLSDFLLWECAFAEFVFLQ
                     KHWPDFTAADLDAAVKEFSRRERTRGALPDAIAG"
     gene            7605..8324
                     /locus_tag="VGR03_14400"
     CDS             7605..8324
                     /locus_tag="VGR03_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995518.1"
                     /translation="MSEFTPHLAPVVALLFFGTILLIGASFLVLFYGTLRRSSFFAKL
                     GAGAAFATAAGYLILLTSVSVASSERVLPPGGWKAFCEIDCHIWYSVAGMQVATALGR
                     EMQQTTANGQFVIARLKTWFNENTISPHRGNRPLVPGPRRVVLVDDSGRSYDVSSEGQ
                     AALAALGDSSLPLDQALRPGESFTTDLVFDIPKTARGLRLLIADDDPATRFVIGHENS
                     LLHKKIYFGVESAPPLTKAAR"
     gene            8420..9100
                     /locus_tag="VGR03_14405"
     CDS             8420..9100
                     /locus_tag="VGR03_14405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV1995519.1"
                     /translation="MGTMGLALPWKARISSTLRERGTRVEPSEHFAGGPSPAVVDATS
                     EDAALAAACRSGDLRAYERLYATHGARMKNLARNVLGNPIDAEDAVQETFLKVQRSIA
                     SFRGQSSFVTWTYRILINTCYDARRSRLRKKEIANDDSEETPRLEPRAPGAHPTLRMA
                     LERALAKLTQHQRDVFLLYEVEGFRHAEIARMLEMTETASKNTLFQAKKNLRQMLEPP
                     RNSAAEAR"
     gene            9097..9777
                     /locus_tag="VGR03_14410"
     CDS             9097..9777
                     /locus_tag="VGR03_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995520.1"
                     /translation="MKIMNVICNDCNDRDRIFEDGTPAEWAALEEHAVFCPLCAEEVR
                     AWKSLSGAAKELRDYSDSPSFWPRIERALAEEAARNAQRAERKSWFSFLPSFSPAWQA
                     ALAGAFVLVLTISGGWLIVHRPDRTIDNDKSLLKSSALMEVESAETVYERAIDKLAAQ
                     TKPQLENPATPLLANYHEKLLLLDSAIGELRAQAGMNPSNAQLRYQLLAMYQEKQHTL
                     EEVLEARQ"
     gene            9777..11426
                     /locus_tag="VGR03_14415"
     CDS             9777..11426
                     /locus_tag="VGR03_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995521.1"
                     /translation="MIRIPSIFRREPQRFAAVAGLLSAMLLFTAKPMRAATSAQDQVS
                     RDFQQTVALGAGQSVRVEHKFGSIRLHGESGRDVKISATIRAQASSHEEAESFAQKIK
                     IEVQQTGEGVRIKTIYPDEEKSWFHSSKHSSWSVSYDIGMPSDAPITVRNSFGSVEVA
                     GVHGAADVENGYGTVNVRDAGAGRWNNAFGSIELAGAGGNVSVSDNNGSVQVSDVKGA
                     LEVRNRFGSITTRNIQGAATITGGNGAVALTDAASANITTSFGSVDARNIRGDLSVRD
                     NNGNVEISGIGGAADITSSFGNVTFADVHGRVNCTTNNGRVKGTSVTGGSVTIRDSFG
                     NIELDTINGALDAETSNGKITVRDARGSVTLKSSFGAIEASNVPKGIRAITGNGGITL
                     SDIGGDTFAKTSFGSVSTERISGNLTVENSNGSVTARNVKGDAGVKTSFAGVTLESIG
                     GRINVDNQNGAISVTAMRPASGCRDISLKTSFSSIRVRVPEGLGYNLTARTSFGRISS
                     ELPVTSTGSIGGDTLSGAIGSGGCQLQLTDSNGSIEIAKAP"
     gene            complement(11439..11912)
                     /locus_tag="VGR03_14420"
     CDS             complement(11439..11912)
                     /locus_tag="VGR03_14420"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1995522.1"
                     /translation="MTSSREYPDRPVVGIGGVIIDQGRTLLIRRGSEPLRGEWSIPGG
                     MLEIGESLEEGVARELLEETGIEVRVLELIEVFDRIYLEDGSTGAEAKRRPRFHFVIA
                     DYLCERLGGEPRAGSDVTDVAFAREEELARFHLTETAIRVLKKAFAMDRARQAAK"
BASE COUNT         2353 a         3592 c         3646 g         2649 t
ORIGIN      
        1 gttcaccgta cctgcgcaat ctgcagttcg atgtcgaagc cctgtcggcg cccgacgcgc
       61 gcatggaatg gcccggcgcg caactgcagg ctgatgcgaa tctccgcgta cggggaacct
      121 gggaacatcc tattctcctc ggacacatcc acatcctgtc gggtgattta tatttcgcgg
      181 gcaaccgtta ccgtgttacg cgcggagact tgaacttcgc caatcccttc cgtctcgatc
      241 cggttttgaa tgtggaagca accacgacga ttcagcaata tgagatcacg ctgaatttta
      301 atggcccggc cagcaagctc acgctggcgt accgctccga tccgcccctt ccggcgaacg
      361 acatcatcac attgctcgct cttgggcaaa ccagctccga ggggaccgcg cgcagcggcg
      421 gtacgtcgca gagcggaacg gcaggcgcct ccgcgattct ctcggaagcg atttccagcc
      481 aattgggcgg gcgcctggag cgcttgtttg ggataacgcg ctttcgcgtt gatccgggtc
      541 ttgcaggagt agactcgacg ggctcggaac agaacgccgc ggcacgcatt accgtcgagc
      601 aacagattac gcccaacctc accatcactt atgtttcgaa tgtgagctcg acccagcagc
      661 aggtcattca agtggaatac aacgtggacc gaaacctctc cattgtcggg ttgcgcgatc
      721 agaacggaac gtttggaatc gacataaaaa tcaaaaagcg cttccggtag ccggaaagtg
      781 cttcccgagt tgcaattcgc attattgcgg atcttgctgt tgcgcggcct gttgctgggg
      841 caattgtccc tatcctcgct gttgtccccg gcgctgctgc cagcggccct ggccctgacc
      901 attcccgagc cgtgccgact gcccaatctc cggattctgc tgcttcaatt ccgccagcgc
      961 atccttggcg gctttggccg tcgctgcacg ggtcgctgcc tgagtttcac ggccgatctt
     1021 gggcatctcc tcggcgacct tctgtcccag cggcgaaccg taaaactgca gcagcgcgtt
     1081 gatttcgtct tcggtgaagt gcttgtcgta aattcccacg agttgatccg ttacctgatc
     1141 gacgtcgaat ttcttctggt agctgacggc gaacgcgttc acaaacgcct ggcctttgtc
     1201 gttgttggga acggtggcga gtagtttttc ccgggactgt tcgatggcgg tgttcgcgcc
     1261 gtcctgcacg aggtcgcgcg caccgaccag ctccatcagc gaacgaatgt ccgcttcttt
     1321 cgccgggtca atggaggaat ttgcttgtgg aggcgccgcg ttgccctgca ccggggtcgc
     1381 gctttgcggc gcggcttgca cggagttctg cgatgctccc tgaatcacca ggaaaaaagt
     1441 gccaacataa ataatgacca gtgccaggac cgctttcatg actgcctccg tggcggcttc
     1501 gcttgtgttt gcgccacgcc tccgatttta ggaagagccg tgcagcagcg cgactgtttc
     1561 gcgggacagt cgtgccgcgt cgttcactgg ccgaacgttc aggtccggga agtggaaatg
     1621 aatttctgct acgatgcctc gccatgaaca agacggaaat gcagggcggc gcatccctgg
     1681 gagcgcctgc acccgctggg gccatcgagc gcgaagcggg tctacagcgc aatctgacgc
     1741 cggggcagct tgccatgatc ggcctcggta gcacgatcgg aacgggcctc tttctaggaa
     1801 gcgctatttc cgtgaagctg gcggggccgg ccgtgattct gagcttcgct ggcgcagcgt
     1861 gtatcgcggt gacgatgatg tgggcgctgg cggagatggc cgcggcgcac ccggcggcag
     1921 gatcgttcgg actctacgcg gagatgtacc tgcatccgtg ggcaggattt gctgttcgct
     1981 tgacttattg gttctgcatg atggtagtgg tgggcagtga agttgtggcc gcttctattt
     2041 acagcagctt ctggtttcct actactccgt cgtgggtttg gatcggcttg ttctcacttg
     2101 tgattcttta tgtcaacacc atcagtatag gaaagttcgg aactttcgag tacatcctct
     2161 ccatgttcaa ggtgataacg attgtggtct ttttgattct ggggaccgca ctgctcactg
     2221 gcgtgggttt tccgaagtta ggttttgcga actatacggc gcacggcggt tttcttccga
     2281 acggatggaa aggcgtcggc ctcggtgtga tcttggggct tttcagtttt ttcggaatcg
     2341 aagttgttgg aagcacagcc ggagaagcag cagatccgaa ggcggcagtg ccgaaggctt
     2401 tgcggagaac gctcttcgct ctggtgctct tttatgtggc aggactggcg ctggtggtcg
     2461 gaattgtccc atggcagcag atcgggctta gcgagagccc tttcgttcgc gttttcaaaa
     2521 ccgttggcat tcccggcgcg agcgacatta tgaatcttgc ggtgctgacg gcggcgcttt
     2581 ccagcgcggt ggccaacttg tacttcgggg cgcggcttgc gttctcgctg tcgcgcggcg
     2641 gttatcttcc ggcgattctt ggacgattga gcaggaaagg catgccggtg atcgcggtgc
     2701 tggcttcggc ggggggaatg gctgcggcac tagttctgtc ggagtacttt aaggattcac
     2761 tgttcgtttt catgatcggg ctcagcacgt ttggggcgct gttcgcgtgg ctgatgacgt
     2821 tattgactca ccttgcgttc cgtcgctttc acaaacggca ggcaagacca taccttcaac
     2881 tggggccgcc tgggccgtgg gcctccctca tgggtttggg aggcgtattg gcagtcttgg
     2941 tgtcaacctg gtgggttccg gaatttcgca ttaccttgct ggccggcata ccctggctgg
     3001 gtttcatcac ggtgtgctat tttgtttgga gaagagcccg atcccgcatg ccaatccaga
     3061 gaggagcgca ggatggatga acttctgaag tggcgcgggg aatttccgat cttggaccgc
     3121 atcacctaca tgatcagcaa ttcgttgggc gcgatgcctc gtggtgtgta cgacgggttg
     3181 cgctcctacg ccgattcctg ggcggaacgt ggagttcgcg catgggagga gggctggtgg
     3241 gaaatggccg tcagcgtggg cgaccagatc gcgccgctga tcggagcggg cccgggcgag
     3301 atttcactcc accagaatgt gacgctcacc caggcggtga tttcctcctg ctttgacttt
     3361 cgcgggcctc gtaacaaggt cgtgatggtc gagcttgagt ttccttcgat tcagtacttc
     3421 taccacgagc aacgccggct cggcgcccga gtcgaagtcg ttccgactcc ggacgacatt
     3481 cgcattgatc tggacaaatt gctcgcggcc atcgacgaaa cgacgctcct cgtgccgatt
     3541 tcgaaggttc ttttccgcag ctcctgcatc gttgacgcgc gcgccattat tgaaagggct
     3601 caccgtgtcg gcgcgcacgt gattctcgat gtttttcagg ctgcgggatc gattcctctc
     3661 gacgtgcgcg gtctcggtgt tgattttgcg gtcggcggag tcctcaagtg gctgtgtggc
     3721 ggaccgggtg tgggttactt gtacgtgcgc gaagatttgc gcgcgaagct ccgccccgcg
     3781 ctcacggggt ggatcgcgca ccgccgccca tttgctttcg aaactggcgc catcgatccg
     3841 cgcgaagatt cattccgcta tctgaacggt acgccacaca ttcctgcgct ctatgcgtgc
     3901 cagccaggac tggagattct taacaaggtt ggcattgcgg cgattcgcga taaatcgatc
     3961 cgcatgaccg cacgcctcat cgagagcgct cggtctcgcg gctggcgcgt gaacacaccg
     4021 gaaaaccctg cggagcgcgc ggggacggtt tcggtcgaat gcccgcatgc ctatgaagtt
     4081 tgccgcgagc tcctcgcgcg ggacatcatc gtcgactacc gtcccaaagc cggtgtgcgg
     4141 gtttcgccac acttctacaa tcgcgaagat gaatgcgact ttacgctcgc gcaaatggaa
     4201 gagatcctga aaacaggcgc gtgggaaaaa catgcggcgg cggttacgca tgcctgaaaa
     4261 aaaagaatac gctgcacagc gtgtggaaat gattgaaaag cagttgcgcc ggcgcggcat
     4321 cagtgacgct gccgtgcttg gggcgatggc ggccgtgcca cggcacgaat ttgttgccga
     4381 ggagctgcgc tctcacgcgt atgatgacct ccccatgccc atcggaggcg gtcaaaccat
     4441 ttcgcaacct tatatcgttg cggccatgac cgcggcgttg catttgcagc caggcgaccg
     4501 ggttctggaa attggcacgg gctgtggcta tcaggccgcc gtgctttcac gtttggccag
     4561 ggaagttttc acgatcgagc ggcgaccgga gcttgcctcc agtgcttccg ccacgcttgc
     4621 tcggctcggc taccccaatg cgcatgtaca ctgcggcgac ggcacactcg gcctggcgga
     4681 attcgctcct ttcgacgcca ttctcgttgc cgctgccgct cctgcggttc cgaagccgct
     4741 ccttgctcag cttgccgaag gcggccgcat gattcttccc gtgggtgatg cggaacatca
     4801 ggaactgcag ctcatcgaaa agcgtggtga cgctttcccc acaaagttgt tggagggctg
     4861 ccgatttgtg ccgctcgtcg gctaccatgg ctggcgggaa ccttcgtaac gatgagctca
     4921 acggcccccg atctttccat catcattccc tcgtacaatg aggaattgcg ccttccggcc
     4981 acgctggagc ggattgcgtc atacctttcg acctacgggc gcgaagcgga agtcctggtc
     5041 gtggacgacg gctcgaagga tggcacggcc gccgtggctg aatattttcg catcaagatt
     5101 cccacgctgc gcgtggtttc gaatggtgtc aaccgcggca aaggctacag cgtgcgccac
     5161 ggcatgcagg aagcgcgcgg ccgcatcgcg cttttcacgg acgcggatct ctccgcgccg
     5221 attgaggaag ccgggaaact ccttgacgct ctggagacct gtgacgtagc gattggctct
     5281 cgagcgatgg accgcagcct gatcagcctg cacgagtctc ccttccgcga attcgccgga
     5341 attattttca ataagatcgt gcgaatcatc ttgtggctgc cgtttgtcga tacgcagtgc
     5401 ggtttcaagg cgttccggcg cgaacgctgc ggcatccttt tcgagcagca gacgattgag
     5461 cgattcgggt tcgacccgga gttgctgtac ctggcgcggc gtcacggcct gcatgccata
     5521 gaaattcccg tgcgctgggg gcattcaccg gccacgaaag tgagcatgct gcacgacagc
     5581 attcagatgt tcatcgacgt attcacgatc cgatggaatt cactgatggg acgttacccg
     5641 cgaaaagcct gattcgcccg cgctttactt tatatcataa agtacttgac aatcctaaat
     5701 ccttgccgta gccttcctgc tatggcgacc tctggtccgc ttcgctctcc gaaacgtgag
     5761 cctatcccca tcgacgctcg cgccgccgat catctgcgct acatccgcga aacgatggag
     5821 agcgccgccg aatttaccgc cgtgcccggc tggggcggcg tggccatggg cgttaccgcg
     5881 ctggtggccg cgtttgctgc ttcgcggcag accacgcctc gcgcctggct aaccgtctgg
     5941 ctgattgaag ccttcgtcgc ggtggccatt gcagcgcccg ctgcggccac caaagcgcac
     6001 cgtgccaact ccgcgctttt ctccgggcct ggccgcaaat tcgttcttag ttttgcaccg
     6061 ccgattgtcg tcggcggcct cctgaccttc gctctctata acgccggcat ccttgcggcc
     6121 ctgccgggtg tttggctcct gttgtatggg accgccatcg ttacgggagg ggcgttttcc
     6181 gtgcgtgttg tgccagtgat gggtttgtgc ctgatgatgc ttggcagcgc tgcacttttc
     6241 gcgccggcag tctggggaga tgccttcatg gccgcgggct ttggggtcgt gcaaatcggc
     6301 ttcggctggt ggatcgcccg gcattatgga ggctagcagg aaatcgcatg gctaagtcgt
     6361 ctacagcgca tcaagaacgt ctgcagcgat ctcgcgagcg cggcgcctcc ttggaagcga
     6421 tccgcaatcc ggcccgggaa accgtgcccg cggatctgga ccggttgatt cacgagcgca
     6481 tccgcttggg tatcgtcagc gcgctggcgg ttaaccgctc gctcactttc aacgaactga
     6541 aagcgctgct gaaaaccacc gacggcaacc taagcgttca cgcccgcaag ctggaagagg
     6601 ccgattacat cgtgtgcacg aaatctttcg acgggcgttt gccgaagacg gaatatcgcc
     6661 tggcggcagc ggggcggcgg gcgctggagc gctacttgaa tcacatggaa gcgctgatcc
     6721 gcgcgacgcg cgaaggttga acggctaaaa tttttggctt ctcactttgt attatagagt
     6781 agtttatgat acttcctccg cggcggagaa agaacatgct catgcatcat ctgacagtac
     6841 ggcaaattcc cacgacccag ccagccatcg cccaggctac ccggctccac gtagccatca
     6901 tctcggacgg caatggccgc tgggccactt cgcggggttt gccgcgctcc gccggtcatc
     6961 gcgcgggagc ggagtccgca cgccgcgtca tcgaggctgg cccccgcatt ggcattcaca
     7021 ccctgactct tttcgcgctt tcctccgcga attggaagcg tcccgctgcc gaggttcatg
     7081 caatcttgcg gctgcttcac gaataccttc ttacagaaac gacgcattgc atcgacgaag
     7141 gtgtgcgggt cagcattatc gggcgccggg atcgcttgcc cgccacgctc cgccaggcca
     7201 tcgcggattc ggaagccgcc acggcaacgg gcacgcgtct gcacctgcgg ctcgcggtgg
     7261 attattccgc tcgcgaagcc atctaccacg cagcctgccg gttttataaa gtcacggagc
     7321 tttcgccgga gtccttcagc actgtacttg cggaagtcca ccgaggggga tcgacggaag
     7381 tcgatttgct gattcgtacc ggcggcgaac agagactttc cgattttctc ttgtgggaat
     7441 gcgccttcgc ggaattcgta ttcctgcaaa aacactggcc ggactttacc gccgccgatc
     7501 tggatgcggc cgtgaaggaa ttcagcagac gcgaacgcac tcgcggtgca ttgccggatg
     7561 ccatcgctgg ttaatttgaa tcaagtcagg gaggaaaaca aaccatgtcg gaattcacgc
     7621 cgcaccttgc ccctgtcgtc gcgcttcttt ttttcggcac gattctgctg atcggcgcca
     7681 gctttctagt gcttttctat ggcaccttgc gccgctcttc gttcttcgca aaactcggcg
     7741 cgggcgcggc attcgcgact gctgctggat atctcatctt gcttacgagc gtttctgtgg
     7801 ccagcagcga gagggttttg ccgccaggcg ggtggaaggc cttttgtgag attgattgcc
     7861 acatctggta ttccgtcgcc ggcatgcaag tggcaaccgc tcttggacgt gagatgcaac
     7921 aaaccaccgc gaacggtcag tttgtaatcg cgcggttgaa aacgtggttc aatgaaaata
     7981 cgatctctcc tcatcgcgga aatcggccgc tggtcccagg tcctcgtcgc gttgtcttgg
     8041 ttgacgactc cgggcggtcc tacgacgttt ccagtgaagg ccaagccgcc ctcgctgctc
     8101 tgggcgattc gtcgcttccc ctggaccagg cacttcggcc gggcgaatcc ttcacgaccg
     8161 atctcgtttt cgatatcccc aagaccgctc gcggcctgcg gctcctaatc gccgatgacg
     8221 acccagcaac tcgcttcgtg atcggtcacg aaaacagcct cttacacaag aagatttact
     8281 ttggcgttga atccgctccg ccgctgacca aggctgctcg ataaaccaat caaaataagc
     8341 agtttgcagg tgcatcccgc accccttcct acccctgaca tttcaccctg ctgcgtcgtc
     8401 tataccccag gagccaggaa tgggaactat gggacttgcg ttgccctgga aggctagaat
     8461 ctcttccacg ctgcgcgaac gggggacgcg agtggaacct tccgagcact ttgccggcgg
     8521 ccccagtccg gccgtcgtgg atgccacgag cgaggatgcc gcactggccg ctgcctgccg
     8581 gtccggcgat ctgcgcgcct acgagcgtct ctacgcgacg catggcgcac gcatgaaaaa
     8641 tctggcgcga aatgttctcg gcaaccccat cgacgcagaa gacgccgtac aagagacctt
     8701 tctgaaagtg cagcgcagca tcgccagctt tcgcggccaa tcgagtttcg tcacctggac
     8761 ctaccggatc ctgattaaca cctgctacga cgcgcggcgc agccgactgc gcaagaagga
     8821 aatcgcgaac gacgattccg aggagacgcc gcgtcttgag ccgcgtgctc ctggagcgca
     8881 tcccacattg cgcatggcgc tggagcgcgc actcgccaag ctcacgcaac atcagcgcga
     8941 tgtttttctt ttgtacgaag tcgaaggttt ccgccatgcg gagatcgcca ggatgctgga
     9001 aatgaccgaa acggcctcga aaaatacgct ttttcaggcg aagaaaaatc tccgccagat
     9061 gctggaacct ccgcgcaaca gcgccgcgga ggcacggtga aaatcatgaa tgtcatctgt
     9121 aacgattgta acgatcgcga tcggattttc gaagatggga cgcccgccga atgggcggcg
     9181 ctggaagaac atgccgtttt ctgcccgctg tgtgcagaag aggttcgcgc ctggaagtcg
     9241 ctgagcggcg cggcgaaaga gctgcgcgac tactcggaca gcccttcgtt ctggccgcgg
     9301 atcgaacgcg cactcgccga agaagcggcg cgcaatgcgc agcgcgccga acgcaagagt
     9361 tggttttcgt tcctgccaag cttttctccc gcctggcaag cagctcttgc tggtgctttc
     9421 gttctagtgc tgacgatttc cggcggttgg ctcattgttc accggccgga ccgcacaatc
     9481 gacaatgaca aatcgctact gaagagcagc gccctgatgg aagtggaaag cgcggaaacc
     9541 gtgtacgagc gggcgattga caagctcgcg gcgcagacca agccgcaact cgaaaatccc
     9601 gcgacaccgc tgctcgccaa ctatcacgag aaactgctcc tgctggatag cgcgatcggc
     9661 gaactgcgcg cgcaggcggg aatgaatccg tcgaatgcgc aactgcgcta tcagttgctg
     9721 gccatgtacc aggagaagca acacacgctg gaggaagttt tggaggctag acaataatga
     9781 ttcgcattcc atccattttc cggagagagc cgcagcgctt cgcagcggtt gcgggactac
     9841 tctccgcgat gctgcttttc acggcgaagc ccatgcgcgc cgcaacctcc gcgcaggatc
     9901 aagtatcgag ggattttcaa cagacggtgg cgctcggcgc gggtcagtcc gtgcgcgtcg
     9961 agcacaagtt cggcagcatc aggctccacg gcgagtccgg ccgggacgtc aagatttcgg
    10021 ccaccatccg cgctcaggcc agctcgcacg aggaagcgga atccttcgcg caaaaaatca
    10081 aaatcgaagt tcagcagacc ggcgaaggag tacgcatcaa aacgatctat cccgacgaag
    10141 agaagagctg gtttcattcc agcaagcact catcgtggtc cgtcagttat gacattggga
    10201 tgccatccga cgcaccgatt accgtgcgga acagtttcgg gagtgttgag gtagcaggcg
    10261 ttcacggagc tgcggacgtt gagaacggct atgggacggt gaacgtccgt gacgccggcg
    10321 ccggccggtg gaacaatgct tttggcagca ttgagctcgc cggggccggg ggaaatgtct
    10381 ccgtgagcga taacaacggc tccgtgcaag tgtctgacgt aaagggcgct ctcgaggtac
    10441 gcaatcgctt cggcagcatc accacacgaa atattcaggg cgcggcgacc attacgggcg
    10501 gcaacggcgc agttgcattg acggatgccg cctcggcaaa catcaccact tcgtttggga
    10561 gcgtcgacgc acggaatatc cgtggcgatc tttcggttcg cgacaacaac gggaacgtcg
    10621 agatttcggg tatcggcggc gcggccgata tcaccagcag ctttggcaac gtcacttttg
    10681 ccgatgtgca tggccgcgtg aattgcacca cgaataatgg ccgggtcaag ggtacctcgg
    10741 tgacaggcgg ctccgtgacc attcgcgatt ccttcggcaa cattgagctg gacaccatca
    10801 acggcgctct agacgccgaa acctccaatg gaaaaatcac ggtgcgcgat gcacgcggga
    10861 gcgtcacgct gaagtcctcc tttggcgcca tcgaagcctc gaacgttccg aagggaattc
    10921 gcgccatcac ggggaacgga ggcatcacac tctcagatat cggcggagat acgtttgcca
    10981 agaccagttt cggaagtgtc tcgacggaac ggatcagcgg caatctgacg gtggagaaca
    11041 gcaatggttc ggtcaccgca agaaacgtaa agggcgacgc cggtgtgaag acctcttttg
    11101 cgggtgtcac gctggaatcc attggcgggc ggatcaatgt ggacaatcag aacggagcga
    11161 tctcggtgac ggccatgcga ccggccagcg gttgccgcga catttcgctc aagacatcgt
    11221 tctcatccat ccgcgtgcgc gttcccgagg gcttgggata caacctcacg gcgcggactt
    11281 ctttcggccg gatttcctct gagttgccgg tcacttcgac gggcagtatc gggggcgata
    11341 cactgagcgg cgcgatcggt tcgggcggct gccagcttca gctcacggat tccaacggaa
    11401 gcatcgaaat cgccaaagct ccctgatcgt gcctcccatt attttgcggc ctgccgcgcg
    11461 cgatccatcg cgaacgcttt cttcaagacg cgtatcgcgg tctccgtcag atgaaatcgg
    11521 gcgagttcct cttcgcgcgc gaaggcgaca tcggttacat cactccccgc gcggggctca
    11581 ccgccgagtc gttcacaaag ataatccgca atgacgaagt gaaatcgcgg tcttcgtttc
    11641 gcctctgcgc cggtcgaccc atcctccagg taaatccggt cgaacacctc gatcagctcg
    11701 aggacgcgaa cctcgattcc agtttcttcc agcaattcgc gggcgacgcc ttcttccaga
    11761 ctctcgccga tctccaacat gccgccgggg atggaccatt caccgcgcaa gggttcgctc
    11821 ccgcggcgga tcagcagcgt gcggccctga tcgataatca cgcccccaat gccaaccact
    11881 ggccgatcgg ggtattctct ggaagaggtc attcgaaagg tccttctgcc cttgatgcga
    11941 tggcgttcaa agcagagcca ccacgcagga gagttgactt catacgccac ggtatataat
    12001 acgctgcaca gtggtgggca cggctctcgt accccccact ttgcaaacga ctgtacggag
    12061 gatggagccg tgatccgctg ccctaaatgt tccaccgaaa tcgatgtgga tgaagacgaa
    12121 gtcgaagaag gcgagatctt gagttgcccc gagtgcgagt ccgagatgga ggtctcgcaa
    12181 acgcatcccg tgcatgtgaa catcatttca gacgatttgg atgacgatga cgacgaggaa
//
LOCUS       DASYVO010000428        23819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_81232, whole genome shotgun sequence.
ACCESSION   DASYVO010000428 DASYVO010000000
VERSION     DASYVO010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 23819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23819
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81232"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..402)
                     /locus_tag="VGR03_14425"
     CDS             complement(34..402)
                     /locus_tag="VGR03_14425"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEV1995523.1"
                     /translation="MKFMMIVKSAEKWGFPPKELMDAIAKLSEDAVKAGSMLGSGGLL
                     PSAKGTSVRISGGQLTVKDGPFTEAKEVVGGYAQFELTSKEVAVESAKRFMELHRQHW
                     PEWEGETEVRQMYEPGDFPH"
     gene            complement(571..999)
                     /locus_tag="VGR03_14430"
     CDS             complement(571..999)
                     /locus_tag="VGR03_14430"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEV1995524.1"
                     /translation="MRFMVIVKANKDSEAGVIPKQKLLAKMGKFNEELVKAGVLLAGE
                     GLHASSKGKRVRFSGENRTVIDGPFTETKELIAGFWLWQVRSMEEAIEWVKRCPSPHE
                     GEAEIEIRQVFEAADFGSELTPELRKGEERLRAQVAAKKP"
     gene            complement(1186..1473)
                     /locus_tag="VGR03_14435"
     CDS             complement(1186..1473)
                     /locus_tag="VGR03_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995525.1"
                     /translation="MTGQSIYSRNRKPIVGVAIVGFAFVTLLCKLDGSTAKGCNLLVK
                     TAWMALEVLRPVIMLADWQAALASLCEDSGFLQHLLQIGESIWPLLCAMAG"
     gene            1976..3460
                     /locus_tag="VGR03_14440"
     CDS             1976..3460
                     /locus_tag="VGR03_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995526.1"
                     /translation="MDQQDFSASPARRSRKSERLLLLLWLFSLPFLNPWVRGDGVGYY
                     AFARAPLIEHNLDFTRDYQFANESFRGPRLDENGQPKSFFRTSTGRLENHFTVGPAIL
                     WTPFLLLAHAGVLLARALGSSVAADGFSAPYRITMAFATAFYAFLGLLLAFRLVRQYV
                     EERWALLATLAIWWASSLPVYMYFNPSWSHAHSSFAVALFLWYWHQTRSSRSLTQWLI
                     LALIAGLMLNVYYPNAMVLAVLAVEALRQYYLAFRPDPSAAPNSTPRVPELLLRHSLF
                     AAVLLVCLLPTFITRYLIYGSPFESGYIPLRDWNWFSPNFLAVLFSSNHGLLAWTPIL
                     LLAIAGLFIFWRWQRGAGAPFLAAFLAFYVFICCYPDWAGISSYGNRFFVSLTPLFIL
                     GLAVFFDRAAQFFRSQRAALAAAIVLLAITSLWNAGFIFQWGSHLIPARGPISFSEMI
                     HNQFFVVPRQLSADLRRYLFKRKALMQQIEGRDIQQLEKNPPPP"
     gene            3516..4385
                     /locus_tag="VGR03_14445"
     CDS             3516..4385
                     /locus_tag="VGR03_14445"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1995527.1"
                     /translation="MASVTQSSVALPLHSGASATIRIEPPRGLFELRLREVWAYRELL
                     YFFVWRDVKIRYKQTAIGVLWVVLQPVLTMLVFTLFFGRLAKLPSQGLPYPVFYFAAL
                     VPWTYFAYALQMTTNVVVDNQRLITKVYFPRLILPISSALSGLVDFSIGFVVLAIFTV
                     AYGFRPTLAALWLPALLALAVFTALGVGLWLSALNALYRDVRYVVPFLIQFWMFASPV
                     VYPASLVPPRWRWLYGLNPMAGVIDGFRWAITGRGQAPGLILLASSAVVALVVLGGLF
                     FFNRMESAVADRV"
     gene            4369..5541
                     /locus_tag="VGR03_14450"
     CDS             4369..5541
                     /locus_tag="VGR03_14450"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEV1995528.1"
                     /translation="MPTESSSGALNLSGRIPELDGLRGLAILLVILCHYVGNPNHAPL
                     GFWPHRFLLAFTAGWSGVDLFFVLSGFLIGGILLDARRAPHYFRAFYMRRVFRILPIY
                     YLWTLLFAATVVAALVFFPGRLGVASSDLLRVPVQLLFLQNIFIGMPHFTWSWFVVTW
                     SLAIEEQFYLLAPPLIRFLSLRRFVVALAATVVLAPVLRFLLFRYWAPGTYLCAFLMP
                     CRADSLSCGMLLAVAWRDARFREFIRARAALLQRLLLVLFLGVGGLLWWLVHPINAIT
                     VTIGYTWLALFYSALLLTVVSQSGGWLAAVMRWRFLGWLGGISYCVYLLHDAFNFFAH
                     AIFFHAEPRLYTLSHFAVTVFALLATLGVASLSWRFFEKPLIRRGHGYSYGEAIAS"
     gene            5544..6359
                     /locus_tag="VGR03_14455"
     CDS             5544..6359
                     /locus_tag="VGR03_14455"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEV1995529.1"
                     /translation="MTASAKHLVVRLFNALGLDIRRRRPATPARASMAGGLAQLARLG
                     FQPRTVIDAGVATQTLELYEPFPSASILLIEPLAEFEPFLRKICSTYHAQYVLAAAGQ
                     APGSATFNVHADKFSSSLLSEVEGPGVDGTPRTVPVVSIDQQCAEKKLSGPFLIKLDV
                     QGAELQVLAGASRTLPQTEAVILEVTLFGTMIGGPQFYDLVTYMKGVGFVAYDIFAVN
                     YRPLDGALAQVDMIFVREDGPFRRSHAFATPEQRDASNREMKAHFAEQAKKLS"
     gene            6481..7695
                     /locus_tag="VGR03_14460"
     CDS             6481..7695
                     /locus_tag="VGR03_14460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HEV1995530.1"
                     /translation="MTTAVIHGEGLGKLYRRGLQVDAGLRHSLEAFIRSPIAALRRKK
                     DETFWALKDVSLEVREGEVLGLIGRNGAGKTTLLKILSRITRPTTGWAEIHGRVGSLL
                     EVGTGFHPELTGRENTFLSGAILGMGKAEIARKFDEIVAFAELEKFIDTPVKHYSSGM
                     YVRLAFAVAAHLEPEILLVDEVLAVGDINFQKKCLGKMGDVARAGRTVVLVSHQLNQI
                     RRLCHRVVWIDAASVRQDGPTHEVVSAYESAMARGESNGNAQARAANSKGRFLTWEIV
                     VPHGDDQYTLTTLGPITVKFTVQLNEPIRNGHHGIALFDAERQLIWAWAAENLKLEIG
                     QHDLCYTFPMLPLRPGPYSWQVSLYEDAECIDTWDCLPEMAVATETHQHQYDQWNGIL
                     NMPSRFKINGRG"
     gene            7698..9263
                     /locus_tag="VGR03_14465"
     CDS             7698..9263
                     /locus_tag="VGR03_14465"
                     /inference="COORDINATES: protein motif:HMM:NF019615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEV1995531.1"
                     /translation="MEKKWLFDSAFTQEYTKIRQAFTGEFLASVRESVDLKSALDVGC
                     GVGYFSKFLADTGFEVVAVDGREENLTEGRRRNPDITFLSKNVEDPALPDLGSFDFVL
                     CVGLLYHLENPFRAVRNLHALTAKVLMLESMCVPGEESNLQLLDESPGEDQGLNYVAF
                     YPTEACLVKMLYRAGFPFVYRFNQLPADEQFTTTLWRKRSRTFLAASNVALTLPNLAL
                     AQEPIRAVPSISDPWTTNLSRARHFLSSKVSSLRVIAGQLRSRMPVPVRLSFGAWWIA
                     RNDHIGRPIREGRFEVAEVAFVRKFLQPGMTVLDIGAHHGFYTLLASKCVGRNGRVVA
                     FEPSSRERKALRLHLKLNRCKNVEVQELALGEEPGKADLHVVDDWAAGCNSLRPPDVA
                     AGTTPVPVSIVRLGDWLADNRIERVDFIKLDVEGGELAVLKGASELLKRPARPIILAE
                     VQDIRTRPWGYGAKEIIEFLRAREYRWFQISDNGSLVELNLNSQVFDGNFVAAPDERM
                     RQVAELAKAESRP"
     gene            9290..10099
                     /locus_tag="VGR03_14470"
     CDS             9290..10099
                     /locus_tag="VGR03_14470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1995532.1"
                     /translation="MDLINTLKEWKNFRKTGVPADSKGPAWKTPFDTLRKKWDQIPTG
                     ASASENTARLLALSDEALLAEWEKARKDITTGPEFTHRGWYHNLYAHGMRGKKVMDVG
                     SGFGVDPITFAQHGAKLTFVDLVETNLKVLERLCKIMGLKDVRFVLLEDLESLRPLDT
                     DYDVIMAMGSLHHAPSDILKPEYQELLRHLKVGGRWLQLAYPRSRWIREGREPFSKWG
                     VITDGPGTPWSEWIEPEKLMDLLAPGKFDLVLHQEFHHGYFNWFDLLYRGY"
     gene            10026..11027
                     /locus_tag="VGR03_14475"
     CDS             10026..11027
                     /locus_tag="VGR03_14475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1995533.1"
                     /translation="MIWFFTRNFITGTLTGLTCSTEGTDLSLIANRATGGTRGADDTA
                     LWPRISVVTAVFNGEIHLEETIRSVLYQEYPNLEYIIIDDGSTDSTPDIIRKYEQHLA
                     WWTSQPNLGMYASLNTGFAKSSGEILGWLNDSDKLHSGALSVVGSAFRDLPQVEWITG
                     RPTGFNEEGMTTLVLDLPHWSRSRFLAGANRYIQQESTFWRRSLWDRAGGHLDASGRC
                     GHVADFELWVRFFRHAQLYPIDTLIGGFRADSKSRTLKDLETYHRLQAEIIAADLAAT
                     PGLHSVKLFRRLNQFAQSVPKLRVLWNRTIMHALYRLPGPDWAPVIRFQHDKWKIWR"
     gene            11155..11499
                     /locus_tag="VGR03_14480"
     CDS             11155..11499
                     /locus_tag="VGR03_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995534.1"
                     /translation="MGSLRDGPIGVRFFRHAKLYPVDALIGGFRAHGDSLGFLDADAS
                     HRTHDEYIAAELAATPGFHSVKLFRRLSRAAQSVPKLRGLWNRTALRALYRFPGPDLP
                     PVIRFDSGRWKL"
     gene            12504..12899
                     /locus_tag="VGR03_14485"
     CDS             12504..12899
                     /locus_tag="VGR03_14485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEV1995535.1"
                     /translation="MSTITRFEDMEAWQTARTLTRTVYQLTASGTFSRDFGLKDQMQR
                     ASVSIMSNIAEGFESRTEVLFLELLGRAKGSAGELRAQFYVAHDAGYISADQFQSIHD
                     QCAKCSAQISRFMAYLKRSNPPSSKTLRR"
     gene            13391..14329
                     /locus_tag="VGR03_14490"
     CDS             13391..14329
                     /locus_tag="VGR03_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995536.1"
                     /translation="MLTLFTIPKSFKGHINVIQRNAIQSWMRLHPEIEIILFGTDEGA
                     AEVAREFGLRHEPQVERNEYGTILLNSVFLKAQQLARYDVVCYVNCDILLLDDFCSAL
                     RTVTAAHKEFLMVGQRTDVDIFNSWPYERAAWQSELRDFAARHGKTRPPSWIDYFAFS
                     RGLYGADLPPFAIGRTHWDDWLVWKSLTAGKPVVDATPVVLAMHQNHDYNHHPQGEAG
                     VWKGLEAARNSHLAGGWGHLRTIAHASEILVAEGLRPNNLRHWAEVKRRFEAVGRFLF
                     YDVWQPVSFAVLNWTRPLRTALGMRAEPLRPPTDKV"
     gene            14402..15766
                     /locus_tag="VGR03_14495"
     CDS             14402..15766
                     /locus_tag="VGR03_14495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEV1995537.1"
                     /translation="MLSSVSVIGLGKLGAPMAACFAARGFTVHAVDLNPQKVDAISRG
                     VPPVHEPGLAELLAESGGRISATKDIEAAVSASDATFVVVATPSEADGGFSLRYAIPT
                     CESIGRALRTKSAYHVVVVTSTVMPGSMGGQIKSALERASGKRCGVDFGLCYNPEFIA
                     LGTVIRDFYNPDFLLIGESDPRAGDVLSEIYKRTCKNSPAIARMNWINAEITKLSVNT
                     YITTKISYANMLARLCEKLPEADVNVVTDALGLDTRIGAKYLKGAVSYGGPCFPRDNR
                     ALAALAARVGASSGLAEATDIFNRAQIKSLADIVKSHRNESDAIGILGLTYKPNTDVV
                     EESFGLLLAQELSSANLPVIVYDPSADAARALNNYKNIRVASSAQECIAESGVVVLAT
                     PWQEFRDLRAALWARRGHSAPRVVIDCWRALSHLDGVEGLRYVKLGFGGAAQKPVSVS
                     SSAD"
     gene            15896..16831
                     /locus_tag="VGR03_14500"
     CDS             15896..16831
                     /locus_tag="VGR03_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995538.1"
                     /translation="MLTIFTTAKPFRGHIGIIQRNALQSWKALHRDIEIILFGADEGA
                     AEAAHEFGLRHEPHVERNEWDSKRLDYLFARAQAIARHELLCYINCDIILMQDFWRAI
                     ERVRAMHRAFLMVGRRWDADITEPLAFEQHDWQSEERQSPDWQKDLRARALQRGRKCT
                     AEWIDYFAFSRGLYGPDMPPFVLRVFWDNWLVWKALDSGKPVIDASRAVMAVHQNHDY
                     SYHAQGKAGIWSGEEAGLNAQLAGGWRHLRTIADATEVLPPEKLKRNALRHWSTAKRY
                     ARQAGRVLLNDVWHPIWFALLNLTRPLRRSLRLRL"
     gene            complement(17216..17413)
                     /locus_tag="VGR03_14505"
     CDS             complement(17216..17413)
                     /locus_tag="VGR03_14505"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEV1995539.1"
                     /translation="MDMRIGLDIAWLATKRLVDAIVLVTGDSDFIAAMKTARKEGIRI
                     YLETLGHPVYVQLKAHADIVL"
     gene            complement(17425..17832)
                     /locus_tag="VGR03_14510"
     CDS             complement(17425..17832)
                     /locus_tag="VGR03_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995540.1"
                     /translation="MSEKTALMVDGGFLKKKLQQQNHRNATVVDITTFCNSVMQKQPL
                     AQAQVLRIYFYDAPPFEGTVTNPLSGVATIMAGTPQAIQNVQLLRNLELQPNFAVRRG
                     VVSCQGWKLGSAALRSISSHPRPVAAGDLVPDL"
     gene            complement(17949..18674)
                     /locus_tag="VGR03_14515"
     CDS             complement(17949..18674)
                     /locus_tag="VGR03_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995541.1"
                     /translation="MGNGEPIDYAAIIADIEAKITALTNTLAAFRAAHAAGALGLSGD
                     GIVPTTTAPFSASGGEVPVGAFLGRSIPEAAKLCLQIVKRKLTSREIADYLKKGGIET
                     SAKSFPALVHSILIRASKPLDSPIVKLDRSYWGLAEWYPTGMRSSGNTDKRIEKRGRK
                     RRRGRPAKSEDGPKLLSGPTPTSRIKEVLKSKPGVEFSAQEIAAPANVSLQVANLTLG
                     RLVASKQAERTHAGKYRAVGSIN"
     gene            19134..19991
                     /locus_tag="VGR03_14520"
     CDS             19134..19991
                     /locus_tag="VGR03_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HEV1995542.1"
                     /translation="MNKLTHEKRVQLVNCLIEGCSIRATVRMTGVAKKTVMRFLVEIG
                     EVCEDYQDRVLRNLSCRRVQLDELWGFNYCKAKNVTPEIAEKIPGAGDVWLWVAIDAE
                     TKLVPCWRLGDRNGGTAFEFVRDLAARLRNRIQLTSDGHRVYLDAVESAFGSGVDYSM
                     LVKLYGADRQESETRYSPAECIGCRTIPIIGNPDPKHISTSFVERQNWSVRTAMRRYT
                     RLSNGFSRKIENHAAAIALNYFAYNFIKIHRSLRVSRAMAAGVTDRLWDASDLVGLLE
                     AEERAERAA"
     gene            20086..21849
                     /locus_tag="VGR03_14525"
     CDS             20086..21849
                     /locus_tag="VGR03_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995543.1"
                     /translation="MLALIIFCILLTWLLCAAVCMGIGGLLLRGFRFPFSRLDAMWTG
                     LAIITGILQLYHFFRPIDLVAVYLLVGVSLAGWFRNRSSLLHFSGVSTFNLPFSNFLL
                     HFTAAAIIAFRCAALGEHYDTGLYGAQAVRWFINYPLVPGLGNVIGQLGFNSSVFLWI
                     AALDQGPWRDLAHHLFAGFLIAALFASIIPAALRIFRAESTSAIDWFFTMLFVPATIW
                     GATSKIVGTNTDLPTSVVCLVAAAILFRALGGESPHAATNPSDGLPPSHGDTTPLGLL
                     IAMVLFSLAITFKISSLVFASAGWTVAVLKLWSLSRNTPMGKRQLVWAVILSAAIVLP
                     WIGRGVVLTGYPFFPSTAFSIPVDWKVPAFETQMQADFARSFARVPDLTYDYAHGWHW
                     LRPWFRELVREREEFLIPLLFALAGGVVGIIRMARHNRNSLPRWLWLLVPSLGGLSFW
                     FLEAPAMRFGEPAIWTVGAALGTFAALHFLNQRGRIRIAVAGLLLLTAWAAHPQLLWS
                     SYFRPSVGVRTFLRLPEAKLTPHQTTSGLTVHVPVETNQCWDAPLPCSPYFNETLHLR
                     QPGKLERGFVSEESGAAVKWK"
     gene            22127..23773
                     /locus_tag="VGR03_14530"
     CDS             22127..23773
                     /locus_tag="VGR03_14530"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEV1995544.1"
                     /translation="MSPSPREISRVREVQPIRLTTEITIHLTPFDQKEYRHLIEARGE
                     TIQRAVTRLKPALELSNAVDAGCGVGFFSQTLAECGLNVCGFDGRGENVAEARRRFPH
                     IPFEQGDIEEREILQLGRFDLVLCFGLLYHLENPLRAIRHLRGLTEKCLLLESMCLPE
                     EKPSMLLREEPRQEDQSLSYVACYASEGSLVKMLYRAGFGMVYRVLPLPDHDDFRETT
                     EHARRRTVLLASAAAIDVAGFRLIPEPDEAEDPWAKNPAATATLPQRIGRFLVSPTRK
                     KYITLANRARRIFPGMPIPLRLPFGAWWLAEKSALDHELIYNEFERMETEFVQRLLRR
                     DMTVVDAGAHHGLYTLLASKRVGWHGRVIAVEPSPRECERLEKHLRMNRCSNTELVPC
                     ALGEDPGEADFYLVDGFQDWCNSLRPPAVDEPVRTVRVSVRRLDDVLAESGVSKVDFL
                     KLDVEGAELSVLYGAMKLLQRKSRPAILAEVQDIRTQPWGYAAREIIQFLIRMDYRWF
                     AIAAKGALLPISCDQETYDANLVALPVERTEEFLSLLGQE"
BASE COUNT         4572 a         7615 c         6194 g         5438 t
ORIGIN      
        1 cgtggcacct ttacgaaaca agccttggtt cggctagtgc gggaagtccc ccggttcata
       61 catctggcga acttcggtct cgccttccca ttccggccag tgttgcctgt gaagttccat
      121 gaagcgcttg gcgctttcga ccgctacttc tttcgaggtc aactcaaact gcgcgtagcc
      181 gccgacaact tctttagctt cggtgaaggg cccgtccttc acggtgagct gcccgccgga
      241 aatccggacg ctggtgccct tagccgatgg gaggaggccg ccgctgccga gcatcgagcc
      301 ggctttgacg gcgtcttcgc tgagcttcgc aatggcgtcc attaattcct tgggcgggaa
      361 gccccatttc tcggcagact tcacaatcat catgaatttc atcgtgacac ctctcctgtg
      421 tgtttttgtt tcccacttcg gagccgggtt tccggacggt ttcgaccggg attgcaaccc
      481 tggctctact gtaacgtcga acgcgggccc atgaaatcga caggctcaat aacttctttt
      541 cgaccggtgt aaggcggggg cgtgccccga ctacggcttt ttggcggcta cctgggcgcg
      601 caggcgctct tcccccttcc tgagctcggg agtaagttcg gaaccgaagt ccgctgcctc
      661 gaagacctgg cgaatctcaa tctcggcttc cccttcatga ggactggggc agcgcttgac
      721 ccactcgatg gcctcttcca tggaccgcac ctgccacagc cagaagccgg cgatcagctc
      781 cttggtttcg gtgaagggcc cgtcgatcac ggtccggttc tctcccgaga accggacgcg
      841 cttgcccttc gaactcgcgt ggagcccctc gccggcgagc agcactccgg ctttcaccag
      901 ctcttcgttg aacttcccca ttttggcaag gagcttctgc ttaggtataa cgcccgcctc
      961 cgagtccttg ttggctttga ctatcaccat gaatcgcatg gttatgtctc cttttgagtt
     1021 cgagttgaga gctgggtttc cggacgggct cgaccgagtg gacctgacct ctactctaac
     1081 gtcgaacgac gggcagtgag atcgacaggt acggtatctt ttttggaagt atttgttaag
     1141 ggttcgcgaa ccataacgcc gcctaggtag gtctctctat tgtgcctagc cagccatggc
     1201 gcaaagcaac ggccagatag actctccaat ttgtagcaaa tgctgcaaaa accctgaatc
     1261 ctcgcaaaga gatgccaacg cagcttgcca gtccgccagc ataatgaccg ggcgcaatac
     1321 ttctaacgcc atccatgctg tcttgaccaa taggttgcac ccttttgccg tcgacccatc
     1381 gagcttacac agcaaagtga caaacgcgaa cccgacgatg gccactccca cgattggttt
     1441 gcggttccga gagtatatgg attgcccagt catctccgcc tcctgcgtca cgctttttcc
     1501 tggctctacc tagatgtcga acgaggggcc gcgagatcga caggttccgt attttactgg
     1561 gaggtatttt tcacgcgttg acggtctttt ggcctttttc gcatcacgtt cgtgttagag
     1621 atgaatggtc gcggactcag agactatcgt gggaatgcag gcaatgccgc ggggctcact
     1681 ggccacatct ccgtgtggtc aagttaccgt acaagcgatc ggcatcgctg agaggaggtt
     1741 acccacaacg ttagagttca ttcgtatggc ttcaaggctc gcgcggatag aagcaaccct
     1801 gttccaactt gccgatatcc aactcccaag taaaatcatg cggtacaatg ccgccacggt
     1861 tatttgcagt taccaggtcc cttcctcggt cttgggagat tggctccaca tcgtgatgct
     1921 gccctgcaat aatggtagcc ggggatagga aaggctttcg gcgggtactt cgttcgtgga
     1981 ccagcaagac ttcagcgcat cgcccgcgcg ccgctcgcgc aaatcggagc ggctgcttct
     2041 ccttctctgg cttttctcgc ttccttttct caatccttgg gtacgcggcg atggcgttgg
     2101 ctattacgct ttcgctcgcg ctccgttgat cgaacacaat ctcgatttca ccagggacta
     2161 tcaattcgcc aacgaaagtt ttcgcggccc gcgcctcgac gaaaatggcc agcccaaatc
     2221 cttctttcgc acatccaccg gccgcctcga aaaccatttc accgtcggcc ccgcgattct
     2281 ctggacgcct tttctcctcc tcgcgcacgc aggcgttctt ttggcgcgcg ccttgggttc
     2341 ctcagtcgcc gctgacggtt tttccgctcc ttaccgcatc accatggctt tcgccaccgc
     2401 gttctacgct tttctcgggt tgcttctggc cttccgcctg gtgcgccaat atgtagaaga
     2461 gcgctgggcg ctcctcgcta cgctcgccat ctggtgggcc agctcgctcc cggtctacat
     2521 gtatttcaat ccttcgtggt cccatgcgca ttcctctttc gccgtggctc ttttcctgtg
     2581 gtattggcat cagacgcgct cctcgcgctc gctcacgcag tggctgattc tcgctttgat
     2641 cgccggcctc atgctcaacg tttactaccc caacgccatg gtcctcgctg ttctcgcggt
     2701 cgaagccctc cgccagtatt acctcgcctt tcgcccggac ccttccgccg cgcccaattc
     2761 cactccgcgc gtccctgaac ttctcctccg ccactcgctc ttcgccgccg ttctcctcgt
     2821 ctgcctgctc cccactttca tcacgcgcta tctcatttac ggcagcccgt tcgaatccgg
     2881 ctacattccg ctcagggact ggaactggtt ttctccgaac ttcttggcag tgctcttctc
     2941 ttccaatcac ggcctgctcg cctggacgcc cattcttctt ctcgccatcg ctggcctttt
     3001 tatcttctgg cggtggcagc gcggcgcggg cgctccgttt ctcgccgcct ttctcgcttt
     3061 ttacgttttc atctgctgtt accccgattg ggccggcatc tcttcttacg gcaatcgttt
     3121 tttcgtttct ctcactccgc ttttcattct cggcctcgcc gttttcttcg accgcgccgc
     3181 tcaatttttc cgttcccaac gcgccgctct cgccgccgca atagttcttc tcgccatcac
     3241 ctctctctgg aacgccggct ttattttcca atggggctcc catctcattc ccgcccgcgg
     3301 ccccatttcc ttttccgaaa tgattcacaa tcagtttttc gtcgtgcccc gccagctctc
     3361 cgccgacctc cggcgctacc tcttcaagcg caaagccctc atgcagcaga tcgagggccg
     3421 cgacatccag cagctcgaaa aaaatccccc gcctccctaa ggctttcata gcggcgggct
     3481 ttagcccggc ctctttctgt tattcttagt cacgcatggc ttccgtcacg caatccagcg
     3541 tcgctctccc gctccactcc ggcgcctccg ccaccattcg cattgagcct ccccgcggcc
     3601 ttttcgagct tcgcctccgc gaagtctggg cctaccgcga gctcctctac ttcttcgtct
     3661 ggcgcgacgt caagattcgc tacaagcaaa ccgccatcgg cgtcctctgg gtcgttctcc
     3721 agcccgtcct caccatgctc gttttcacat tattcttcgg tcgcctcgcc aagctcccct
     3781 cccaaggcct cccctatccc gttttttatt tcgccgcgct cgtcccctgg acctatttcg
     3841 cctacgccct ccagatgacc accaacgtcg tcgtcgacaa ccagcgcctc atcaccaaag
     3901 tttatttccc acgcctcatt cttcccattt cttccgcgct ttccggcctg gtggacttct
     3961 ccatcggttt cgtggtcctc gccattttca ctgtcgcgta cggcttccgc cccactcttg
     4021 ccgcgctgtg gctgcccgcg ctgctcgctc tcgcggtctt caccgctctc ggcgtcggct
     4081 tgtggctctc cgcgctcaac gctctttatc gcgacgtccg ctacgtcgtt cccttcctca
     4141 tccagttttg gatgttcgcc tctcccgtcg tctatcccgc ctcgctcgtt ccgccgcgct
     4201 ggcgctggct ctacggcctc aaccccatgg ccggcgtcat tgacggtttc cgctgggcca
     4261 tcaccggccg cggccaggcc cccggcctca tcctgctcgc ttccagcgcc gtcgtcgccc
     4321 tcgtcgtcct cggcggcctc ttcttcttca atcgcatgga gtccgccgtt gccgacagag
     4381 tctagctccg gcgcgctcaa tctctccggc cgcatcccgg agcttgacgg cctccgcggc
     4441 ctcgccatcc ttctcgtcat tctttgtcac tacgtcggca atcccaacca cgccccgctc
     4501 ggtttctggc cgcatcgttt tcttctggcc ttcaccgccg gctggagcgg cgtcgacctc
     4561 ttcttcgtcc tctccggctt cctcatcggc ggcatcctcc tcgacgctcg ccgcgccccg
     4621 cactacttcc gcgcctttta catgcgccgc gtcttccgca tcttgcccat ctattacctc
     4681 tggacgcttc ttttcgccgc caccgttgtc gccgcgctcg tctttttccc gggccgcctc
     4741 ggcgttgcct ccagcgatct cctccgcgtt cctgtccagc ttctcttcct ccagaacatt
     4801 ttcatcggca tgccgcactt cacttggagc tggttcgtcg tcacctggtc tctcgccatc
     4861 gaagaacagt tttaccttct cgctccgccg ctcattcgct tcctttcgct tcgccgcttc
     4921 gtcgtcgcgc tggccgccac cgtggttttg gctcctgtgc tgcgcttcct gctcttccgc
     4981 tattgggctc cgggaaccta tctctgtgcc tttctcatgc cttgccgcgc ggattcgctc
     5041 tcctgtggca tgcttctggc ggtggcctgg cgcgacgccc gttttcgcga attcatccgc
     5101 gctcgcgccg cgcttctcca gcgcctcttg ctcgtcctat tcctcggcgt gggcgggctg
     5161 ctctggtggc tggtccaccc catcaacgcc attaccgtca ccatcggcta cacttggctc
     5221 gccctttttt acagcgccct cttgctcacc gtcgtttcgc aatccggagg atggctcgcc
     5281 gccgtcatgc gctggcgctt tctcggctgg ctcggcggca tctcctactg cgtctacctg
     5341 ttacacgacg ctttcaactt ttttgcccac gccatctttt tccatgccga gccgcggctc
     5401 tacactctca gccatttcgc cgtcaccgtc ttcgccctgc tcgccactct cggcgttgcc
     5461 tcgctctctt ggcgcttctt cgaaaagccg ctgatccgcc gcggccacgg ctatagttac
     5521 ggggaggcca tcgcctcttg accatgaccg catccgccaa acatctcgtc gtccgtctct
     5581 tcaacgcctt gggtctcgac atccgtcgcc gccgtcctgc tacgcccgct cgcgccagca
     5641 tggccggagg cctcgcccag cttgcgcgcc tcggcttcca gccccgcacc gtcatcgacg
     5701 ccggcgtcgc cacgcaaacc ctcgagctct acgaaccgtt cccctccgcc tccatcctcc
     5761 tcatcgagcc tctcgccgag ttcgagccct tcctccggaa aatctgctcc acctaccacg
     5821 cccaatacgt tctcgccgcc gccggccagg ctcccggctc cgccaccttc aacgtccacg
     5881 ccgacaaatt cagctccagc cttctcagcg aagtcgaagg cccgggcgtc gacggcaccc
     5941 cccgcaccgt ccccgttgtc tccatcgacc agcagtgcgc cgaaaaaaaa ctctccggcc
     6001 cctttctcat caagctcgac gtccagggcg ccgagctgca ggtcctcgcc ggcgcctccc
     6061 gcaccctccc gcaaaccgaa gccgtcatcc tcgaagtcac cctcttcggc accatgatcg
     6121 gcggcccgca gttctatgac ctagtcactt atatgaaagg agttggcttc gtggcctacg
     6181 acattttcgc cgtcaactac cgccccctcg acggcgccct cgctcaagtc gacatgatct
     6241 tcgtccgcga agatggcccc ttccgccgct ctcacgcctt cgccactccc gagcagcgcg
     6301 atgcttcaaa tcgcgaaatg aaagcgcact tcgccgagca ggccaagaaa ctctcatgac
     6361 caacgctgtc attcacggag aaggactcgg gtttaagtgg cacagccatt cctggctgtg
     6421 ctccttcctt tgtagaggcg caatatattg cgccctctct tctcctgagg ctcctcgtcc
     6481 atgaccaccg cggtcattca cggtgaagga ctcggcaagc tctatcgtcg cggtttgcag
     6541 gtggacgccg gtctccgcca ttccctcgaa gccttcatcc gctcacccat cgctgcgctt
     6601 cgcagaaaaa aggacgaaac gttctgggcc ttgaaagacg tctcccttga agtccgcgaa
     6661 ggcgaagtcc tcggcctcat cggccgcaac ggtgcgggca agacgactct gctgaagatt
     6721 ttgtcgcgca ttacccggcc cactaccggc tgggccgaaa tccacggccg cgtcggctcc
     6781 ctcctcgaag tcggcaccgg cttccatccc gagcttaccg gccgcgaaaa tacgttcctc
     6841 tccggcgcta tcctcggcat gggcaaagcg gaaatcgccc gcaaattcga cgaaatcgtg
     6901 gccttcgccg aactcgaaaa attcatcgac actcccgtca agcactactc ctccggcatg
     6961 tacgtccgtc tggcctttgc cgtcgccgcc catctcgagc cggaaatcct tctcgtcgat
     7021 gaagtcctcg ccgtcggcga tatcaacttc cagaaaaaat gtctcggcaa aatgggcgac
     7081 gtggcccgcg ccggccgcac cgtcgttctc gtctcccatc agctcaatca gattcgccgc
     7141 ctttgccatc gtgtcgtgtg gatcgatgcc gcctccgtcc gccaggacgg ccccacccac
     7201 gaagtcgtct ccgcctacga atccgccatg gccaggggtg agtccaatgg gaatgcgcaa
     7261 gcccgcgccg ccaattccaa aggccgcttc ttgacgtggg aaatcgtcgt gccgcacggc
     7321 gatgaccagt acacactcac taccctcggc cccatcaccg taaaatttac tgtccaactc
     7381 aacgagccca tccgcaacgg ccatcacggc atcgctctct tcgacgccga gcgccagttg
     7441 atctgggctt gggctgcgga gaacctgaag ctggaaatcg gccagcatga cctctgctac
     7501 acttttccca tgctcccgct gcgtcccggc ccgtattcct ggcaggtcag tctctacgaa
     7561 gacgccgagt gcatcgacac ctgggattgc ttaccggaaa tggccgtggc cacggaaacg
     7621 catcaacatc aatatgatca atggaacggt attctcaaca tgccgtcgcg ctttaaaatc
     7681 aatggcagag gataaaattg gaaaagaaat ggctattcga cagcgctttc actcaggaat
     7741 acaccaagat aaggcaggca ttcaccggcg aattcttggc gtcggtccga gagagcgtgg
     7801 acttgaagtc ggcattggac gtcgggtgcg gagtcggcta tttttccaaa ttcttggccg
     7861 acacgggatt cgaagttgtc gcggtggatg gccgggagga aaatctcact gaagggagaa
     7921 gacgcaatcc ggatattact tttctttcta aaaacgtcga ggatccagcc ctgccggacc
     7981 ttgggagttt cgacttcgtt ctatgcgtcg gtctcttgta tcaccttgaa aacccgtttc
     8041 gcgcggttcg aaaccttcat gctcttacag ccaaagttct gatgctcgaa tcgatgtgcg
     8101 tcccaggcga agaatccaac ctacagttgc tcgatgaatc tccgggggaa gaccagggcc
     8161 tgaactatgt ggcgttctat cctaccgaag cttgcttggt gaaaatgcta tatcgcgcgg
     8221 gctttccgtt cgtataccgc tttaatcagc taccagcgga cgaacaattt acgaccaccc
     8281 tttggcgcaa aagatcgcgg acgttcctgg cagcgtcgaa cgtcgccctg acactcccta
     8341 atcttgctct cgcacaggag ccgatccgag ccgtgcccag catatcggac ccttggacca
     8401 ccaatttgtc cagagcacgg cactttctta gctctaaagt gtcttcctta cgagttatcg
     8461 cggggcagtt gcggtcgaga atgcccgtac cggtcaggct gtctttcggc gcatggtgga
     8521 ttgctcgaaa cgatcatatc ggaagaccaa taagagaagg tcggtttgag gttgcagaag
     8581 tcgccttcgt ccgcaaattc ctccagcctg gcatgaccgt tctcgatatc ggcgcacatc
     8641 atggcttcta caccctgctc gcgtcgaagt gcgtcggccg aaatgggaga gtggtcgcgt
     8701 tcgagccctc atcccgcgaa agaaaggccc tgcgcctaca cctgaaactg aatcgttgca
     8761 aaaacgtcga ggtccaggaa ttagcacttg gtgaagaacc tggaaaagcc gatctccatg
     8821 tcgttgacga ctgggcggcc ggctgcaaca gtctcaggcc tccagacgta gcggcaggta
     8881 ccacccccgt cccggtcagc atcgtcaggc ttggcgactg gctcgccgat aacagaattg
     8941 agcgagtgga cttcattaag ctagatgttg aaggcggcga gctcgcggtt cttaaggggg
     9001 catccgaact cttaaagagg ccagcccgtc ccatcattct tgcggaagtc caggacatcc
     9061 gtacccggcc atggggatac ggcgcaaaag aaatcattga gttcttgcgc gcgagggaat
     9121 acaggtggtt ccagatttca gacaacggtt cactcgttga gctaaatctg aattcgcagg
     9181 tgtttgacgg taacttcgtt gcggccccgg acgagcgtat gagacaagtc gcggagctag
     9241 cgaaagctga atctcgcccc taacctgagc acaggggcaa tctcgagcta tggacctgat
     9301 taacacactc aaagaatgga aaaacttccg caaaactggc gtgcccgccg actccaaggg
     9361 cccggcgtgg aaaacaccat tcgacaccct gcggaaaaaa tgggatcaaa tccctaccgg
     9421 cgcgtccgca tccgagaata ccgccagact actagctctt tctgatgaag cactcctggc
     9481 cgaatgggag aaggcccgca aagacatcac gaccggcccg gagtttaccc atcgcggctg
     9541 gtatcacaat ctctacgcgc acggcatgcg tggcaagaaa gtcatggacg tcggcagcgg
     9601 ctttggcgtt gaccccatca ccttcgcgca gcatggcgca aaattgacct ttgtcgatct
     9661 cgtcgaaacc aatctgaagg tccttgagcg tctttgcaaa atcatgggcc tgaaggatgt
     9721 ccggttcgta cttttggaag acctggaatc actccggccg ctcgataccg actacgacgt
     9781 catcatggcc atgggctccc tgcatcacgc tcccagcgac atcttgaagc cagaatatca
     9841 ggaactcctg cggcatttga aggtcggcgg gcgctggctc cagctcgcct atccccggag
     9901 ccgctggatt cgtgagggcc gcgaaccctt cagcaagtgg ggcgtaatca ccgacggccc
     9961 gggaacaccc tggtccgagt ggatagagcc ggagaaacta atggacttgc tcgcaccggg
    10021 gaagtttgat ttggttcttc accaggaatt tcatcacggg tactttaact ggtttgacct
    10081 gctctaccga gggtactgat ctgtctctga ttgcgaatcg ggcgactgga ggcacacgcg
    10141 gggcggacga cacggctttg tggccgagga tatccgttgt caccgctgtc ttcaatggag
    10201 agatacacct tgaagaaacg atccgttctg tcctttacca ggaataccca aacctcgaat
    10261 acatcatcat agacgacggg tccacagatt ccacgcccga catcatccgc aagtatgagc
    10321 agcatctcgc ctggtggacc tcgcagccga atctaggtat gtacgcctcg ttgaataccg
    10381 gatttgcgaa atcctcggga gagattctgg gttggctcaa tgacagcgac aaactgcata
    10441 gcggcgcgct ttccgtagtc ggcagcgcct ttcgcgatct tccccaggtc gaatggatca
    10501 ccggccgtcc caccggcttc aatgaagagg ggatgacaac actcgtgctc gatcttccgc
    10561 actggtcacg ctcccgcttc ctggccggtg ccaatcgcta cattcagcag gagtccacgt
    10621 tctggcgccg ctccctttgg gacagggcag gaggccatct ggacgcatcc ggacgatgcg
    10681 gacacgtagc cgactttgag ctttgggtgc ggtttttccg ccatgcccaa ctctatccaa
    10741 tagacacttt aataggtggt ttccgcgctg acagcaagtc ccgcaccctc aaggacctgg
    10801 agacctatca tcgccttcaa gctgagatca tcgccgccga ccttgccgcc acccccggcc
    10861 tgcactccgt gaaacttttc cgccgcctca accaattcgc ccaatccgtc cccaaacttc
    10921 gcgtcctctg gaaccgcacc attatgcatg ccctgtaccg cttaccaggc ccggactggg
    10981 cgcctgtcat cagatttcaa cacgacaaat ggaaaatttg gcgctagtct gagtccggta
    11041 cgtccacgaa agggcgagcc tcatgcgttt gatcaatagc acaaggagga gttacaaact
    11101 ccttcgactg gggatcgccg ttatcctcgg cctctgcgcc ggtgctgtta tgttatgggt
    11161 tctctccggg atggcccgat aggggtccgc ttcttccgcc acgcaaaact ttatcccgtt
    11221 gacgccctca tcggcggctt ccgagctcac ggcgattccc tgggatttct ggacgcggac
    11281 gccagccatc gcacccacga cgaatacatc gccgccgaac tcgccgccac tcccggcttt
    11341 cactctgtga aacttttccg ccgcctcagc cgcgccgcgc aatccgtccc caaactccgc
    11401 ggcctctgga accgcaccgc cctccgcgcc ctctaccgct tccccggccc tgatcttcct
    11461 cccgtcattc ggttcgactc tggacgctgg aagttgtagc atatttcccc cagcttagga
    11521 gtttcccacg aacgcactcg cccatgacgt taccgcgcat tcgatgaggg cggtcgtttg
    11581 cggcgtgccc acttgatgaa ttttaccgtg agaagaatga cagcgaagag aattgccaca
    11641 gcaaaagcgc actggacgag gaaaacggaa aattcgccga agtcataccc accgctcatt
    11701 atcgtatccc ccgattgaat tgcttcgtcg tcttcatcca tcccaattat agcgctctct
    11761 atcgcttccc cggccccgac cttccccccg tcattcggtt cgactctgga cgctggaagt
    11821 tgtaatatac ctacccccag cttaggagat tcccatggac gaacccaccc ataggcatca
    11881 atgtcctatt tgccccagtt gcggtagtca cagaactcac cgatcgttga gaaagggccc
    11941 ccatgagtgg gtccttcaca gtctgctatt tcaaagtccc tatcgctgca acgcctgcga
    12001 tcaccgcttc ttcggctttc gtgtacccca tcaccccaag aaagatccgg accaccacca
    12061 cgaaccacga accaccaacc attaacgact tccatacacg cccccaacat tcccccgtca
    12121 tgcggttcca ctctggccgc tggaagttgt agtatagttt tgtccagctt aggagttccc
    12181 catgcccgaa ctcacaacta aggatgaatc tcccatttgc cctcattgcg gtaacgacaa
    12241 aatctaccgc tcgcgaagaa atggcccccg agaatggttc cttcactact tgctatctaa
    12301 gagcccctat cgctgccagg actgcaaggc acgcttcttc cactcgcgct tcgcccatca
    12361 ccccgaaaaa gaactgaacc gccaccccgt ttaacccgtc ctcaaacgtc ctaacgttaa
    12421 gacgttgtta tggcgttccg ccgatccgcc cttttttctt gacgttcaga ccttccgacg
    12481 ttaagacgtg tagacgtgta tttatgagta cgattacgcg attcgaggac atggaggcct
    12541 ggcaaacagc ccgcaccttg accaggactg tctaccagct cacggcgagc ggcaccttct
    12601 cccgcgattt cggtttgaag gaccagatgc agcgagcttc cgtttccatt atgtccaata
    12661 tcgcagaggg tttcgagtcc cgcacggaag tcttattcct cgaactcctc ggccgcgcca
    12721 agggctccgc cggcgagctc cgcgcccaat tctacgtggc tcacgacgcc ggatatattt
    12781 cggcggatca gttccagtcc atccacgatc agtgcgcaaa atgcagtgct caaatcagcc
    12841 gcttcatggc ctatttgaag cgctccaacc ctccttcttc gaagacgtta agacgttgag
    12901 acgttccgtt tccccttctt cgaagacgtt aagacgttga gacgttccgt tcccccttct
    12961 tcgaagacgt taagacgatc cgccgcccta tccccattcc cctcaacatt ttacacttat
    13021 atccacatag taattgacat gccctttact tctgtgctat tctctttcgt gtttgtcgct
    13081 caccaacccc gccgagcccc attgtcccgt caccgtggca aaaaacacgc cgccgtaact
    13141 cctttagatg ccacacttct ggacttcctt gtaagtgtgg taaacaaaag acttacagaa
    13201 actctaagtc ctttagttgc cacacatccg aaaaagaggg ggtggggggt gttattgtta
    13261 accagattcc cgataaggga atctgtcctg aggagcatcg cgacgaagga cctctctgcg
    13321 catcacttct ccgctatccc ctatttccta ccccctatcc cctctcttcc ctttgcgcta
    13381 ttctcaaaac gtgctcaccc tcttcaccat cccgaaatcc ttcaaagggc acatcaacgt
    13441 catccagcgc aacgccatcc agagctggat gcgcctgcat cccgaaatcg aaatcattct
    13501 tttcggcacc gatgaaggcg ccgcggaagt cgcccgcgaa ttcggcctcc gccacgagcc
    13561 tcaagtcgag cgcaacgaat acggcaccat cctcctcaat tccgtcttcc tcaaagcgca
    13621 gcaattggcg cgctacgacg tcgtctgcta cgtcaattgc gacatccttc ttctcgacga
    13681 tttctgctcc gcgctccgca ccgtaaccgc cgcccacaaa gaattcctca tggtcggcca
    13741 gcgcaccgac gttgacatct tcaattcctg gccctacgaa cgcgccgcgt ggcaatccga
    13801 actccgcgac ttcgccgccc gccatggaaa gacgcgcccg ccgagttgga tcgactactt
    13861 cgccttctcc cgcggcctct atggcgccga cctgccgccg ttcgccatcg gccgcaccca
    13921 ctgggatgac tggctggtct ggaagtccct caccgccggc aagcccgtcg tcgacgctac
    13981 ccccgtcgta ctcgccatgc accagaacca cgactacaac caccaccccc agggcgaggc
    14041 cggcgtttgg aagggcctcg aagccgcccg caactcccac ctggccgggg ggtggggcca
    14101 cctccgcacc atcgcccacg ccagcgaaat tctagtcgcc gaaggtttgc ggccgaataa
    14161 cctgcggcac tgggccgaag tgaaacgccg tttcgaggcc gtaggacgat ttcttttcta
    14221 cgacgtttgg caacccgtct cgttcgccgt gctgaattgg acgcgcccgc tccgcactgc
    14281 gctgggcatg cgcgcggaac ccctccgtcc ccccaccgac aaagtctgac tcttttcgcg
    14341 ctcgcaggct ttctgccagc ggcttctcac gctagactag ggattctcta tcaggaggcg
    14401 catgctctcg tccgtatcgg tgattggttt gggcaaactc ggcgcgccca tggcggcttg
    14461 tttcgccgcg cgcggcttta ccgttcacgc cgtcgatctg aatccgcaga aggtggacgc
    14521 catcagccgc ggcgtgccac ccgtacacga acccggcctc gctgagctgc ttgccgaatc
    14581 cggtggccgc atttccgcca cgaaagacat tgaagccgcg gtcagcgcct ctgacgccac
    14641 cttcgtcgtc gttgccactc ccagcgaagc ggatggcggt ttttcgctgc gctacgccat
    14701 ccccacgtgc gaatccatcg gccgcgccct gcgcaccaaa tccgcgtatc acgtggtcgt
    14761 cgtcaccagc acggttatgc ccggctccat gggcggccag atcaaatctg cgctcgaacg
    14821 cgccagcggc aagcgttgcg gcgtcgactt cggcctgtgc tacaacccgg agttcatcgc
    14881 gcttggcacc gtcatccgcg atttctacaa tccggatttt cttttgatcg gtgaatccga
    14941 tccgcgcgcc ggcgatgttt tatccgaaat ctacaagcgc acctgcaaga actctcccgc
    15001 cattgcgcgc atgaattgga tcaacgcgga aatcaccaag ctctccgtca acacctacat
    15061 caccaccaaa atcagctacg ccaatatgct ggcgcggctc tgcgagaaat tgccggaggc
    15121 cgacgtgaat gtcgtcaccg atgccctcgg cctcgatacg cgtatcggcg ccaagtattt
    15181 gaagggcgcc gtgagttacg gaggaccatg ctttccccgc gataaccgcg ctctcgccgc
    15241 gctggcggct cgcgtcggcg cttcctccgg ccttgcggaa gccaccgaca ttttcaatcg
    15301 cgcgcaaatc aagtccctgg cggacatcgt gaagagccat cgcaacgaat ccgacgccat
    15361 cggcattctt ggacttacgt acaaacccaa taccgatgtc gtcgaagaat ccttcggcct
    15421 cctgctcgcg caggagctct cgtcggcaaa tcttccggtg attgtttacg acccctccgc
    15481 cgacgccgcg cgcgcgctga acaactacaa gaacattcgc gtggcctcat cggcgcagga
    15541 atgcattgcg gagtccggag tggtggtact ggcgactccc tggcaggagt tccgcgatct
    15601 gcgagccgcg ctatgggctc gtcgcggcca ttccgcgccg cgcgtggtca ttgattgctg
    15661 gcgcgcactg agtcacctgg atggcgtcga aggtcttcgc tacgtaaaat tgggattcgg
    15721 cggcgcggcg cagaagcccg tcagcgtctc ttccagcgcc gactaatctc gcccgtgatt
    15781 gccacgctcg ccgtctcccc accgtctcgc cttccacctt gaaatcctgt ggcttttaaa
    15841 tatatgcagt gctatattct agcggcgtta tctatgataa taagacgtta ccttaatgct
    15901 aactatattc acaacggcaa agcccttccg cgggcacatc ggcatcattc agcgcaacgc
    15961 gctgcaatcc tggaaggcgc ttcatcgcga catcgagatc attctctttg gcgcggacga
    16021 aggcgccgcc gaagccgcgc acgaattcgg actgcgccac gagccacacg tcgagcgcaa
    16081 cgagtgggac tctaagcggc tggattatct cttcgccagg gcgcaggcca tcgcgcgtca
    16141 cgagctcctc tgctacatca attgcgacat catcttgatg caggatttct ggcgcgccat
    16201 cgagcgcgtg cgcgccatgc accgggcatt tctgatggtc gggcgccgct gggacgccga
    16261 tataaccgag ccgctggcgt tcgagcagca cgattggcaa tccgaggaac gccaatctcc
    16321 ggattggcaa aaagacttgc gagcgcgggc actacagcgc ggccgaaagt gcaccgcgga
    16381 atggattgat tacttcgcgt tttcgcgcgg actgtatggg ccggacatgc cgccgtttgt
    16441 gctgcgcgtg ttctgggaca actggctggt gtggaaagcg ctggactcgg ggaagccggt
    16501 gatcgatgct tcgcgcgccg tgatggcggt gcaccagaat catgactaca gctaccatgc
    16561 gcaagggaag gcaggcatct ggagcgggga agaagcgggg ctcaacgcgc aacttgccgg
    16621 aggatggcgc cacctgcgga ccatcgccga tgccacggaa gtccttccgc cggagaagct
    16681 gaagcgcaac gcgctgcgcc actggtccac cgcaaaacgc tacgccagac aagcgggccg
    16741 cgtccttctt aacgatgtct ggcaccccat ctggtttgct cttctaaatc tcacccgccc
    16801 cctacgccgc tccctccgtc ttcggctgta gctcaaaccg gaacgagtcg caacctcgtt
    16861 gggaggggga ggccggtagt gggctacttt gacccagacc ccctctagaa ttcccttgct
    16921 agtcatggca cactgtagcc atgccaaagc ggattagcaa acccaaacgg aaaaagagag
    16981 aacaggaatc agacgtaaac caacttgcgt accgtctggt tcaggaatcc acttcggaac
    17041 caaacgagga agccttgacg aaaacgcaga tttcgcaatt aatggccgcg atgggcagga
    17101 agggcggcaa gatcgggggc aagcggcgaa tggtgaccat gacaccagaa gagcgcagcc
    17161 aagctggtct gaaggcggct caggttcggt ggaagcgagc aaaagccaag tctactcaca
    17221 agacgatatc agcatgagct tttaactgaa cgtagacagg atgtcccaag gtttccaaat
    17281 atattcttat cccttccttg cgggccgttt tcatggccgc aataaaatcc gaatctccag
    17341 taactaagac aattgcgtca acgagccgtt tcgtcgccag ccaagcgata tcgagaccaa
    17401 tccgcatgtc cacacccttc tgctttataa gtcagggact aagtctcccg ccgctactgg
    17461 acgagggtga cttgaaatag acctgagcgc ggcgctacca agtttccatc cctgacaaga
    17521 aaccacgccc ctgcgaacgg caaaattagg ctgcaattcc aaattacgaa gcaattgcac
    17581 gttctggatc gcttgcggcg tacccgccat gattgtggcg actccactca atggattcgt
    17641 aaccgttcct tcaaaaggtg gcgcatcata gaaataaatc cgcaacactt gcgcttgggc
    17701 aagaggctgc ttttgcatga cgctgttgca aaaggtcgtt atatccacca ccgtggcgtt
    17761 cctgtgattc tgctgttgca gctttttctt gagaaagcca ccgtcaacca taagtgcagt
    17821 cttttcggac acagccaact ccttgcctct tgacaccccc ctaccgtggg ggatactttg
    17881 gaccttgaga ttgccccctg ctacgcccct gatctaagtg ggcttcggcc ccgcgtagaa
    17941 gggggcttct agtttataga cccgactgct cgatatttcc ctgcatgcgt cctttcggcc
    18001 tgtttgctgg cgactaacct gcccaatgta agatttgcga cttgcaggga aacatttgcc
    18061 ggtgcagcta tctcttgggc tgaaaactcc acaccaggct tgctcttgag aacctctttg
    18121 attcggcttg taggagtagg tccgctcaga agtttcggtc catcctccga tttggccggg
    18181 cgtccacggc gacgcttccg gcctcgcttc tctattcgct tatccgtatt gccggagcta
    18241 cgcataccag tggggtacca ctcagccaaa ccccaatagg agcggtctaa tttaacgatg
    18301 ggactatcga gcggctttga tgctcgaatc aagatagagt gcacaagggc tggaaaactc
    18361 tttgccgaag tctcaattcc gccctttttc agataatccg caatctcccg actggtcagc
    18421 ttccgcttga cgatctgcaa gcagagtttg gctgcctcgg ggatgctcct ccctaggaac
    18481 gctccgactg gtacctctcc gccgctggcc gagaacggtg cggtcgtagt cgggacaatc
    18541 ccgtcaccag acagccccag agcgccagcc gcatgagcgg cgcgaaacgc tgccaacgta
    18601 ttggtcagcg ctgtaatttt ggcctcaata tccgcgataa ttgccgcgta atcaataggt
    18661 tccccatttc ccataaatcc tcccttccat tcctacaaaa cgtgtgttat aaaacacctg
    18721 agggcatgag agtcagggcg gctttcgccg ccctggagct ctcgcgggcc tatccgcccg
    18781 ctaggactct catatagcta ccctcagttc tgatgctctg ggccccggtt cctcttggaa
    18841 aagtgatcgg ggccttgagc tttcatcttc ttttacaccc ttctcgaaaa aagcgcaagc
    18901 ccaatttcaa catttttcgt ctggcccaag gcgtattgac acagtcagcc caaggtgaag
    18961 atgccactac gttgccgctg ccacgccatt ctagcgccat ttcgcagatt acctaggtat
    19021 cccactttca cccactttcc acagcccttt ccacagaacc cgtgaaacga ttagtaatcg
    19081 ctattaaata ccgcttgaca atgctaaagc ggctttagta gtattatccc accatgaaca
    19141 agcttacaca tgaaaagcgc gtccaattgg tgaactgcct tatcgaaggg tgctcaatcc
    19201 gggcgacggt gcgcatgact ggcgtagcca aaaagacagt tatgcgtttc ctcgttgaga
    19261 tcggcgaagt ctgcgaggac taccaagatc gcgtgctccg caatctctca tgcaggcgcg
    19321 tccagcttga cgaactgtgg ggattcaact actgcaaagc caagaatgtt acgcccgaaa
    19381 tcgccgagaa aattcctggt gctggcgatg tatggctctg ggtggcaatt gatgcggaga
    19441 cgaaactggt tccgtgctgg cggcttggcg accgcaacgg cgggacggca ttcgaatttg
    19501 tgcgcgatct ggcggcgcgt ttgaggaatc gcatccaatt gaccagcgac ggccatcgtg
    19561 tttatctgga tgccgtagaa agtgcattcg gttctggggt ggattattcg atgctcgtca
    19621 agttgtacgg tgccgatcgg caagagagcg aaacgcgcta cagtccagca gagtgtattg
    19681 gctgccgtac cattcccatc atcgggaatc ctgacccgaa gcatatcagc acaagtttcg
    19741 ttgagcggca aaattggagt gtgcggacgg ccatgcgacg ctacacgcgg ctgtccaatg
    19801 gtttctcgcg gaagattgaa aatcatgcgg cagcgatagc cttgaactat ttcgcctaca
    19861 acttcattaa gattcatcgg agcttgcgag tgagtcgagc aatggccgct ggagtgacag
    19921 accgtttgtg ggatgctagt gatctcgtcg gcctgttgga agccgaagag agggcggaaa
    19981 gagcagcata atgggtgtta cgccgctaca aaatcaaagt agcccactac cgggaggcct
    20041 gttaatttcg aaaccgaggc tcgggcctgc tagaatctcg cctcgatgct agctctcatc
    20101 attttttgca tcctactcac ctggctcctc tgcgccgctg tgtgcatggg gatcggggga
    20161 ctgttgcttc gcggcttccg cttcccattt tctcgcctgg acgccatgtg gaccggactg
    20221 gccatcatca ctggcatcct gcagctctat cacttcttca ggcccatcga tctcgtggct
    20281 gtctatctcc tggttggggt gagcctcgcc ggatggtttc ggaatcgatc ctcgctcctt
    20341 cacttttccg gcgtttcaac tttcaacctt ccattttcaa actttcttct tcacttcacg
    20401 gctgcggcca tcatcgcctt tcgctgcgcc gctcttggcg aacactacga caccggcttg
    20461 tatggcgcgc aggccgtgcg ctggttcatc aactacccgc tggttcccgg cctcggcaat
    20521 gtaatcggac agctcggatt taattcttcc gtcttcctct ggatcgcggc gctggatcag
    20581 ggcccgtggc gcgatttggc gcaccatctt ttcgccggat ttctaatcgc tgcgcttttc
    20641 gcttctatca ttcccgctgc tcttcgtatc ttccgcgccg agagtacttc tgcgatcgat
    20701 tggttcttca caatgctctt tgtccccgca acaatctggg gcgccaccag caagatcgtc
    20761 ggcaccaaca ccgacctgcc cacgagtgtg gtctgcctcg tcgccgccgc catcctcttc
    20821 cgcgccctcg gtggcgaatc cccccacgca gccacgaacc ctagcgatgg tctccccccg
    20881 agccatgggg ataccacccc cctgggcctg ctgattgcca tggtgctgtt ttcgctggct
    20941 atcacgttca agatttccag cctggtcttc gcctcggcgg ggtggaccgt agccgttttg
    21001 aaattgtggt cgctgagccg caacactcca atgggaaaac ggcagttggt gtgggcggta
    21061 attctgtccg ctgcgattgt gcttccgtgg atcgggcgtg gcgtggtgtt gacggggtat
    21121 cccttttttc cgagcacggc tttttcgatt ccggtggatt ggaaagtgcc ggcgttcgaa
    21181 acgcaaatgc aggcggactt tgcgcggtcg ttcgcgcgcg taccggatct tacttacgat
    21241 tacgcgcacg ggtggcattg gctgcggccg tggttccgtg agctggtgag agaaagagaa
    21301 gaatttttga ttccactatt gtttgcgctg gcgggcggcg tggtggggat cattcgaatg
    21361 gcacgccaca atcggaattc gcttccgcgg tggctttggc ttctcgtgcc ttcgctgggt
    21421 ggattgagct tttggttttt ggaagcgcca gcaatgcgct tcggtgaacc ggcgatctgg
    21481 actgtgggag cagcgctggg aacgtttgcg gcgcttcatt ttctgaacca acggggaagg
    21541 attcgaatcg cggtggcggg cttgctgttg ctgaccgcgt gggcagcgca cccgcagctt
    21601 ttgtggagtt cctacttccg tccaagcgtt ggcgttcgaa cgttcctgcg cctgccggag
    21661 gcaaaactga cgccgcacca aaccacttcc gggctgacgg tccacgtgcc cgtggagacg
    21721 aatcaatgct gggatgcgcc gctgccctgt tctccttatt tcaatgaaac gctgcacctg
    21781 cggcagcccg ggaaattgga acggggattt gtttccgaag aatcaggcgc agccgtgaag
    21841 tggaagtgaa gggaattgcg gcgcgcgcaa aactccagcg cccgggccaa cctgtttgcc
    21901 agccggtact aaaaaagaat ctacttgcgt aattaactgg atgtaattag catgccacta
    21961 ctgcatgtag ttagtacgac cgtctatcgg gttcctaagt attttcgttc ctggcaccgg
    22021 cagccgaatc ggctgatcca gcgacacgtc agtctctgaa ctggtgggac cctccccacc
    22081 gcgagactgg ctgtaccaga aatgcagcag taaggaggcc gatccaatga gccccagccc
    22141 gcgagaaatt tcccgtgtcc gggaggtcca gcccatccgg ctgaccacgg aaatcaccat
    22201 ccatttgacg ccgttcgatc agaaggagta tcggcatctg attgaggcgc ggggcgaaac
    22261 catacagcgc gcggtgacac ggctaaagcc cgcgctggag ctttcgaatg ccgtggatgc
    22321 aggctgcggc gtcggattct tttcgcagac gcttgcggag tgcggtctga atgtgtgcgg
    22381 gttcgacggg cgaggagaaa acgtggcgga agcacggcgc cggttcccgc atatcccttt
    22441 cgaacaggga gacatcgagg agcgggagat cctgcagttg ggccgattcg atctcgtttt
    22501 gtgcttcgga ctgctctacc acctggagaa tccactgcgg gcgattcgcc atctccgcgg
    22561 gctgacggag aagtgcctgc tgctcgaaag catgtgcttg ccggaggaaa agccctcgat
    22621 gctgctgcgc gaggagccgc ggcaggaaga tcagagcctt agctatgtgg cttgctatgc
    22681 gagcgagggc agcctggtga agatgctgta ccgcgcgggt ttcggaatgg tttatcgcgt
    22741 attacccctg ccggatcacg atgattttcg cgagacaacg gaacacgcac ggcgccgcac
    22801 cgttttgctg gcatccgctg cagcgatcga cgtggccggg ttccggctga ttcccgagcc
    22861 agatgaagca gaagatcctt gggcaaagaa ccctgcggca acggccacat tgccgcagcg
    22921 catcggacga tttctcgtta gccccacgcg aaagaagtac atcacgctgg cgaatcgcgc
    22981 gcgccgcatt tttcccggaa tgccgattcc cttgcggctg ccgttcggtg cgtggtggct
    23041 ggcggagaaa agcgcgctgg atcacgaact gatctacaac gagtttgaac ggatggaaac
    23101 ggagtttgta cagcgcttgc tgcggcggga tatgaccgtc gtggatgccg gggcgcatca
    23161 cgggttgtac accctgctgg cgtcgaagcg agtcggatgg cacggccgtg tgattgcggt
    23221 cgagccctca ccgcgggagt gcgaacggct ggagaaacac ctgcgcatga accgctgttc
    23281 gaacaccgaa ttggtgcctt gcgcgctggg agaagatccg ggggaggcgg acttttatct
    23341 ggtggatgga tttcaagatt ggtgcaacag cctgcggccg ccggcggtcg acgagcccgt
    23401 ccggacggtg cgggtctcgg tgcggcggct ggatgacgtg ctggcggaat caggcgtgag
    23461 caaagtggat ttcctcaaac tcgacgtgga aggggcggaa ctctccgttc tgtatggggc
    23521 aatgaagctg ctgcagagga aatcgcggcc ggcgattctg gcggaagtgc aggatattcg
    23581 aacgcagccg tggggctacg ccgcgcggga aattattcag tttttgattc gaatggatta
    23641 ccgctggttc gcgattgcag cgaagggcgc gctgctaccc atttcctgcg atcaggaaac
    23701 gtatgatgcg aacctggtag cgctgccggt ggagcgcacg gaggaatttc tcagcctcct
    23761 cggacaagag taagacttac tccccgaggt aagccacaga gcaggcttag atgcggagt
//
LOCUS       DASYVO010000429        12875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_66401, whole genome shotgun sequence.
ACCESSION   DASYVO010000429 DASYVO010000000
VERSION     DASYVO010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 12875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12875
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66401"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(773..1711)
                     /gene="era"
                     /locus_tag="VGR03_14535"
     CDS             complement(773..1711)
                     /gene="era"
                     /locus_tag="VGR03_14535"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV1995545.1"
                     /translation="MNTSEEKSFRSGFVAILGRPNAGKSTLLNRFVGQKIAIVTSKPQ
                     TTRNRIQGIVNRSDGQIVFIDTPGLHLADSQLGRQMVNEIAAALDGVDLLLLVVDASW
                     EAAGVDETLIQRARGFGGATILALNKIDQVPKRKLLPLIDSFRKAFDFAAIVPISALK
                     GDGCEELLQEVMNRLPEGEPYFGKDQVTDQPERFLAAEIIREKAIRLTYHEVPHALAV
                     VVDQFDESPKLVRIHATLKVERDSQKKILIGSKGAMLKRVGTEARKEMETLLGTKVFL
                     ELYVKVVPNWRENPQEVRQLNWHEQLKELGGGESEE"
     gene            complement(1731..3017)
                     /locus_tag="VGR03_14540"
     CDS             complement(1731..3017)
                     /locus_tag="VGR03_14540"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEV1995546.1"
                     /translation="MPGSWLYALSVILVAAGLPVFSYLAIVHRELGRATTGRVHEHLE
                     IYEAEIEPRFHLDRRHAARSFRLIAHFWLAFLILEMTRGVVYFVPGTWEALLEFLVFL
                     GVAVIVGMHFLPDMLLYRTTGRWLIPLVPLIRVFTWLAWPVRVFLDGAESLARISEQE
                     PSAADKEQADSEAIGAMVDAAEEEGILEPGQADLIEQLVEFSDKRVRELMTPRPDIVA
                     IAADATLQKLRKRFVETGFSRIAVFEKTLDDIFGAAFAQDLLQIPEQEMSRRRVRELV
                     RHVLLVPETKSGSDLLQEMRRKNERMAVVIDEHGSVAGIVTVEDLVEEIVGEESQPPA
                     DVRRGPDGAYFVRGSFSISDIEELLGIHFGESSDETVTTIAGLLSHVLGRVPAAGDHM
                     DIEGHRFEVLEANQRKVLRVRIRRLEARAAADGTPA"
     gene            complement(3017..3466)
                     /gene="ybeY"
                     /locus_tag="VGR03_14545"
     CDS             complement(3017..3466)
                     /gene="ybeY"
                     /locus_tag="VGR03_14545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV1995547.1"
                     /translation="MIVNRQRLARIPRRSLELFLRRLQAELKLGETGVTVSFVSDAEI
                     ARMNRVFRGKRGPTDVLSFPAKSRRKPRLHRAGSASRRADGYAGDIAISTAAARRNAA
                     EFGRAFPTELRILILHGVLHLVGYDHETDQGQMERAERKLRRRLGLH"
     gene            complement(3463..4479)
                     /locus_tag="VGR03_14550"
     CDS             complement(3463..4479)
                     /locus_tag="VGR03_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV1995548.1"
                     /translation="MRRTVNITGKIEPLFGALDENLRLLEDALEVQATLQDRSLTIEG
                     QHAQVERAARIIEEYNHLSRGGRTLSGSEVKSLIRVAAEEPGIDPRRVLDSGNARSFG
                     KKNVAPKNASQRHYMEAIERHDMTFSIGPGGTGKTYLAVAMAVSALLTKQVNRIILVR
                     PAVEAGERLGFLPGTLQQKIDPYMRPLYDALYDMLDADKLERFLEKGIIEVAPLAFMR
                     GRTLNDSFVILDEAQNTTSEQMKMFLTRLGFNSKAVITGDVTQIDLPPGKRSGLVEAL
                     EICGGIEGIAMVHFNEKDVVRHNLVQRIIRAYELHDAQPGARDGSAEAGRPATAGKKE
                     ELRG"
     gene            4968..5429
                     /locus_tag="VGR03_14555"
     CDS             4968..5429
                     /locus_tag="VGR03_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995549.1"
                     /translation="MNMNRIQRLLIAVVVILGLFALPHAAVAKGKAEHHNGKQLLGDK
                     IKTNGHHVIHKKGKYTTSVEVKDGKIAGVHVNHTKKGDVPVKKYKTNKNMGQADIHQF
                     VPFDLGQDQYLGTIWIGFAYYDDYGVETIYWFPYDMILDGITGAIDYVPAY"
     gene            5679..5849
                     /locus_tag="VGR03_14560"
     CDS             5679..5849
                     /locus_tag="VGR03_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995550.1"
                     /translation="MKRSIALFLRMPALRIELRNGKKFVFLLGIQGHEEIVDAVEKAI
                     RDMDSQHAIVFK"
     gene            6083..7627
                     /locus_tag="VGR03_14565"
     CDS             6083..7627
                     /locus_tag="VGR03_14565"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV1995551.1"
                     /translation="MMSGPNAKRLKVHLINPSHVSFGTAVITPRWLYVLAAATPASFG
                     DPILVDETLSRAKPERIQRGDVVGIGIHTGNALRGYEIGKMARERGAYVVFGGIHATL
                     YSEEAQQLGGAHAVVKGDGDIVWATVLADCAKGEPKPIYLGGRIEPSDFFPARWDLLP
                     PKSYMWASVQTVRGCPKHCSFCSVWRTDGQRPRQRTSDAVIEEIVQLRRLGFRFVALA
                     DDNFYPVTLTDLALATRQGDLHRLQELTELRAERFELMERMTHLPRDMVFYTQITMEA
                     AEDTTFLDAMRRANIKGALVGVESVTPEGLKDIFKEFNAAGADLVERLKTFRRHGVHV
                     LGSFIFGLPSDRPETFEATAAVAEQAEITFAQFVMLTPFPGTVDFARWEKGLESDSTR
                     IGGFPLTRHWLIPQDLRPKLYCAHPVMPAEEIRRRTQGVWDRFYSLRLIWNRSRCVKS
                     LPSRLAFVLISKLYRQMYANTGIATDSARAARSARWARWLAKPTRRLFSGRPMPELQV
                     PAAVGV"
     gene            complement(7797..9374)
                     /locus_tag="VGR03_14570"
     CDS             complement(7797..9374)
                     /locus_tag="VGR03_14570"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1995552.1"
                     /translation="MSNQLPTTPPQALPQKQVSAALRPGRSGASGKRDKLDELRLLVN
                     SHHPLITIETAEELRVEELLCDVATELGVVFYVWSVTTGLARRGGQPLYNTEQPEQAL
                     ANLALVPGDAIFLLKDFARYLEQDRICRRVRELAEGFRAARRSIVISGASLHLPEELA
                     GDAVPYQMSLPDADELLPVVREVLAEASHASNLAGGLDAATMQELARNLVGLPREEAR
                     RLLQMCLLARGKDAAGLLTDIRDAKQRALRQDSLLEVLPRDASFGQVAGLVHLREWIG
                     KRRSALTAEGRAFGLEPPKGVLITGVQGCGKSLAARAIAGEWGFDLARLDAGALYDKF
                     IGESERRLRKALELAQKLAPMVLWIDEIEKAFAGAGAGAETDAGLSQRLLATMLTWMQ
                     ERESGVFLAATSNNIGSLPPEMLRKGRFDEIFFVDLPAEPIRESLFALHLKKRGRDAG
                     QFDLAKLGGASEGFSGAEIEQAIVAGLYTAFGRKLQLSTEILLGEIHATQPLSVTRAE
                     DIQALREWAKSRAVPAD"
     gene            complement(9611..10285)
                     /locus_tag="VGR03_14575"
     CDS             complement(9611..10285)
                     /locus_tag="VGR03_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995553.1"
                     /translation="MDANQPSTKRCRFCAEEIQADAVKCRFCGQWLTEQPAPAPSAAV
                     QPAAAAPPAAGQGVDAFQLGGAYQPQFDVNRLSPAQREAYKKNYLLETFSVGGAIALH
                     IFTLGIFTIIYMGLKHGKLPQILSDDPSAGKAIGFLFIPFFNIYWVFFFWLRLADRVN
                     FQFRLRGQPLPISRGLVLAAVIVSIIPYVGMISWLVLYPIVISEVLGACNQLAVANQS
                     AGLAAR"
     gene            10467..10763
                     /gene="rpsT"
                     /locus_tag="VGR03_14580"
     CDS             10467..10763
                     /gene="rpsT"
                     /locus_tag="VGR03_14580"
                     /inference="COORDINATES: protein motif:HMM:NF013787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV1995554.1"
                     /translation="MALGQATKIKKRKKSVLKRARQSVERAAVNRSNRTQVRTIMKRM
                     RAAIGAGDKTAASSLFAPTLSAIDRAISKGVLHENTGNRYKSRIAIAFNGLQAK"
     gene            complement(10856..11947)
                     /gene="holA"
                     /locus_tag="VGR03_14585"
     CDS             complement(10856..11947)
                     /gene="holA"
                     /locus_tag="VGR03_14585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1995555.1"
                     /translation="MARVTTEGLLTRLAKGKPLPAIFLLGDELYLRDACRVLLIEKYV
                     PEEARAWAVSRFSAADGETVAALSQAQTLPMLSPQQLVFLEDVQAIEDLGDKKRDEVV
                     KAIEAYLANPAPFTTLVLEASALDQRMRLAKLLAEKTLVVAVSLGDEAVPRRASAVAE
                     ANRLAAELGVKLDSGVADELADLVADNLLQMKMELEKLAAHAGESRRIRLEDVAALVV
                     SNKKSTVWAMAGMIAGQQREAALEFLDRLLRDGEEPVAVVGAVAWMYRKLLEAQEIKG
                     PVNAWQVARQLGMRPEMAEIALSNAPRISRAQLLSGIGALQECDDRLKGGSRDARAAL
                     DFLIARLAAPERAVLAPKTAVVPTNHLSN"
     gene            complement(11947..12480)
                     /gene="lptE"
                     /locus_tag="VGR03_14590"
     CDS             complement(11947..12480)
                     /gene="lptE"
                     /locus_tag="VGR03_14590"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEV1995556.1"
                     /translation="MRRFWKVTAICALQLTAAFAAGCGYHVAGRTSSLPAAIRTIAVP
                     AFENKTTRYRIEQRLTEATVHEFLARSRYRIVSDASNADAVVHGKVASIEVTPLLFDT
                     TTGRATTMLVSVRCEVALEEVATKRELYRNNNFLFRNQYEITADVKSFFEEEDPALDR
                     LARDFASRLVAALTEGY"
     gene            complement(12556..>12875)
                     /locus_tag="VGR03_14595"
     CDS             complement(12556..>12875)
                     /locus_tag="VGR03_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995557.1"
                     /translation="AGVIASLVHGFKIPLHNPRWAIAWLLLAAALGALMTSTIRFYSF
                     KDLPWRRKLPSLTIILVVLLVGLIWKYSEFVLVIIACTYAAAGVVLHIIRFLRQRLVS
                     RTA"
BASE COUNT         2572 a         4133 c         3670 g         2500 t
ORIGIN      
        1 tcttccgggc acgctttcga ccgcggagca ttgcgaccag tcaagtgcca tcttcactcc
       61 cctcggccat tctagcgtaa agtccgagcg gccagcacgc gaaatcgcac tcgccgcagg
      121 ccggtgcctc acgttccccc cgctcccacg ccgtggcagg atctcagccc actcccccga
      181 aaatcaagcc gttgacccaa cctcttccac ttcaatgact tacacaaatt ccttgacaat
      241 gagctaactc ttgtggtaac atttcttgtg agttcgataa gctttcagtc ccgcttggcg
      301 ggacgaatcc tgagcgcagg gccgctctct ccggcccgca tccgatcaaa gattccgcgc
      361 ttcgcggaaa gggcgcttca ggtcgcctta ccgtccccgt tcgttacgtc atcgatttct
      421 gtgctccgcc gttttcccct ccgaaagcgt tacccgaaac cttgtggaat caacacttgc
      481 aaaagtgtat caaaacaaag aacttcaacc gcctttagaa tcaacactta caaaaaacag
      541 ggggggaggg aggtatctcc tctgtcaaca gtcccagtac cccgctcctg gcggaaaagg
      601 gccacttcgc atccgaactc tgtggcctct gtgccctctg tggtgaattc cctttcagag
      661 tatccggcca ggtctttcgc ctcctctacc tcatttacct cctccacttc ctttacctcc
      721 accttccccc ccaccagtca ccagtcacga atcatcagtc accaatcccc gctcactcct
      781 cgctctctcc gcctcccagc tccttgagct gctcgtgcca gttgagctgc cggacctcct
      841 gcggattctc ccgccagttc ggcaccacct tgacgtacag ctccagaaaa acctttgtgc
      901 ccagcagcgt ttccatctcc ttccgcgctt ccgtgccgac gcgtttgagc atggcgccct
      961 tgctcccgat caggatcttc ttctgcgaat cgcgctccac tttcagcgtc gcgtggattc
     1021 gcacgagctt gggcgactcg tcgaattgat ctaccaccac cgccagggcg tgcggcactt
     1081 cgtggtaggt aagccggatc gctttctccc ggataatctc cgcggccaga aatctttccg
     1141 gctgatccgt cacctggtct ttcccgaagt acggctcccc ttccggcagc cggttcatca
     1201 cttcctgcaa gagctcctcg cagccatcgc ccttcagtgc gctgatgggg acaatcgccg
     1261 cgaagtcgaa agccttccgg aaggaatcga tcagcggcag gagcttccgc ttcggcactt
     1321 ggtcaatttt gttcagcgca agaatagtcg ctccgccgaa gcccctcgcg cgctgtatca
     1381 aagtttcgtc gacgcccgcc gcttcccagc tggcatccac gaccagcaaa agcaaatcca
     1441 cgccatcgag cgcggccgcg atctcgttta ccatctgccg gccgagctgc gaatccgcca
     1501 ggtgcagccc cggcgtgtcg atgaatacaa tctgcccgtc gctgcgattt acgatgcctt
     1561 gaatccgatt gcgcgtcgtc tgcggcttcg aagtgacaat ggcaatcttc tgtcccacaa
     1621 agcggttcag cagcgtggac ttcccggcat tcggccttcc gagaatcgcc acaaatcccg
     1681 accgaaacga tttttcctcg ctcgtgttca cagggccatc aatctttcct tcacgccgga
     1741 gtcccgtcgg ctgcggctct tgcttccagg cgtcgaatcc gcactcgcag cactttccgc
     1801 tgattcgcct cgagcacttc aaaccggtgg ccctcgatgt ccatgtgatc gcccgctgcg
     1861 ggcacccggc cgagaacgtg gctcaggagc cccgcgatgg tcgtgaccgt ttcatcggag
     1921 ctctcgccga aatggatccc cagcaactct tcaatgtcgg agatcgaaaa gctcccccga
     1981 acgaagtacg ccccatccgg cccgcgccgc acatccgcag ggggctggga ttcttcgccg
     2041 acgatttcct cgaccaggtc ttccaccgtg acaatgcccg cgacggagcc gtgttcgtct
     2101 atgaccaccg ccatgcgctc gtttttccgc cgcatctcct ggagaaggtc cgagccgctc
     2161 ttggtttccg gcaccagcag cacgtgccgc accagctccc ggacgcgccg ccggctcatc
     2221 tcctgttccg gaatctgcag caagtcctgc gcgaacgccg ctccgaaaat gtcgtccagc
     2281 gtcttctcga aaaccgctat ccgggaaaat cccgtctcga cgaagcgctt ccgcagtttc
     2341 tggagcgtcg cgtccgcggc gatggccacg atgtccggcc gcggagtcat cagttcgcgc
     2401 acgcgcttat cgctgaactc caccaactgc tcgatcaaat ccgcctgccc aggctccagg
     2461 attccctctt cctccgcggc atccaccatc gccccgatcg cttcgctgtc ggcctgctcc
     2521 ttgtccgcgg cgctgggctc ctgctcggaa atccttgcaa gcgattcggc tccatccaga
     2581 aatacgcgca ccggccaggc cagccacgtg aagactcgaa tgagcgggac gagtggaatc
     2641 agccagcgcc ccgtggtccg gtaaagaagc atgtccggaa ggaaatgcat tccgacgatg
     2701 acggcgactc ccaggaacac gagaaactcg agcaaagcct cccacgttcc cggaacgaaa
     2761 tagacgacgc cgcgcgtcat ttccaggatc aggaaagcca gccagaaatg cgcgatcagg
     2821 cgaaacgatc gcgcggcgtg gcgcctgtcg aggtgaaacc gcggctcaat ctcggcttcg
     2881 tagatttcca gatgttcgtg cacccggccc gttgtggcgc ggcccagctc ccgatgcacg
     2941 atggcgaggt aggaaaagac cgggagtccc gccgcaacca gaatcacact cagcgcataa
     3001 agccaggatc cgggcatcag tgcaaaccca ggcgccggcg cagtttccgt tcggcgcgtt
     3061 ccatctggcc ctggtccgtc tcgtgatcgt agccgacgag gtgcaacacg ccgtggagga
     3121 tgaggatgcg aagttccgtt gggaacgccc ggccgaactc agccgcgttg cgccgggcgg
     3181 cggccgtcga gatggcgatg tcgcccgcgt aaccatccgc gcggcgcgaa gcgctccctg
     3241 cgcgatgcag cctgggcttt cttcgcgact tcgccgggaa cgaaagcacg tcggtcggac
     3301 ctcgcttgcc gcggaacacg cggttcatcc gggcgatctc cgcatcgctc acgaagctga
     3361 cggtcacgcc ggtctccccc aatttcagtt ccgcctgcaa gcgccggaga aagagttcaa
     3421 gagagcggcg cggaatccgc gccaaccgct ggcggttcac aatcatcccc gcagttcttc
     3481 ttttttgccg gcagtggccg gccttccggc ctcagccgat ccgtcgcgcg cccccggctg
     3541 cgcatcgtgg agttcgtacg cgcggatgat gcgctgcacc aggttgtggc ggaccacgtc
     3601 cttctcgttg aagtgcacca tcgcaatccc ctcgatgcct ccgcagatct ccagcgcttc
     3661 caccaaaccc gagcgcttcc ccggcggcag gtcaatctgc gtgacgtcgc cggtaatcac
     3721 ggccttcgaa ttgaagccca gccgcgtgag aaacattttc atttgctcgc tggtggtgtt
     3781 ttgcgcttcg tccagaatca cgaacgaatc gttcagcgtc cttccccgca tgaacgccag
     3841 cggcgccact tcgatgattc ccttctccag gaaccgctcg agcttgtcgg cgtccagcat
     3901 gtcgtacagc gcgtcataga gcggccgcat gtacgggtca atcttctgct gcagcgtgcc
     3961 cggcaggaat cccagcctct ctcccgcctc caccgccggc cggaccagaa tgatgcggtt
     4021 gacctgtttc gtcagcagcg ccgacacggc catggccacc gccaggtaag tcttccctgt
     4081 tccgcccggc ccgatgctga acgtcatgtc gtgccgctcg atggcctcca tgtagtgccg
     4141 ctggctggcg tttttcggcg cgacgttctt cttcccgaac gatcgcgcgt tcccggagtc
     4201 caggacgcgc cgcgggtcga tgccgggctc ttcggccgcc acacggatga gcgacttcac
     4261 ctcgcttccg gaaagcgtac gcccgccgcg cgaaaggtgg ttgtattcct cgatgatcct
     4321 cgccgcccgt tcgacttgtg cgtgctggcc ctcgatcgtc aggctgcggt cctgcaacgt
     4381 cgcctgcact tccagcgcgt cctcgagcag gcgcagattc tcatcgagcg cgccgaaaag
     4441 gggttcgatt tttcctgtga tgttgacggt gcgtcgcatc caattggtgg gtctgctgag
     4501 ggaggccatt gagccgatgg gtgatcccta acctttagag agtgatccct ccgcctcgaa
     4561 gtacaaccgc attttcaggg tagcgccaca ccggactccc ggtcaagccg ggtggcccaa
     4621 gcgcgagccg gggggaagtc gagggtgccc cacacctcga ttttatgtgt gggagttctc
     4681 gccctgatcc gcccgcaccg cacgcgtcct catccactcc cgcaacgact ggatatctat
     4741 cacccgagtc accgaagacg gatgcgacta tgcgaccaat atcgcaatgt gttcctttct
     4801 gaacagaaac attgtttcct ggtgactaat atcttgactt ggcggtcgac ccaagcggtg
     4861 gtcaacgcca agcggaggta atcgacgggt ccagcgtgcc agcctctgcg ggctgatccg
     4921 catacaggga cttaacgcca ccgcacactc aagactggag ataaaccatg aacatgaacc
     4981 gtatacagcg tcttctcatt gccgtcgtcg tcattctcgg cctgttcgcc cttccccatg
     5041 ccgcggttgc aaagggcaag gcggaacatc acaacgggaa gcaactgctc ggcgacaaga
     5101 tcaaaaccaa cgggcaccat gtgatccaca agaaaggaaa gtacaccact tcggtggaag
     5161 tgaaagacgg aaagatcgcg ggggtacacg tcaaccacac caaaaaggga gacgtgccgg
     5221 tcaagaagta caagaccaac aagaatatgg gccaggcgga tatccatcag ttcgtgccct
     5281 tcgacttagg ccaagatcag tatctgggca cgatttggat cggctttgcc tactacgacg
     5341 attacggagt cgagacaatc tattggttcc catacgacat gatcctcgac gggattacgg
     5401 gcgcgatcga ctacgttcca gcgtattaag cggcagctct ctccagcagg ctgcggaaaa
     5461 attcgcgagt ggcccatcca tcccgcccca gcgggaaggg tgggggacac ttctcatccg
     5521 agaggtggct gccgtctttt ctcagcccct tgatatatcc cgaaattgca tctctggcat
     5581 tttcttagcc gctgcgaatg agtggcggta cccgattcgg cagcgggccg agagattcct
     5641 tcgccttccc ggtcagtgac gtacggtccg tcgaaagaat gaaaagaagc attgccctct
     5701 ttttgcgtat gcctgccctg cgcatagaac taagaaacgg gaagaagttt gtttttcttc
     5761 tgggcattca gggacacgaa gagatcgtgg acgccgtcga gaaagccatc cgggacatgg
     5821 attcgcagca cgcaatcgtc ttcaagtgac gacgcgatca agcgccgtca tgctgtttag
     5881 cctgagcttc gaagggcctg ccccgaacgt ctttcgcgcg tggcgaccgt agattccgct
     5941 cctgacggaa aggaagcgag cacaacgagc cgggcttgcc acctaagcac ggccagggcg
     6001 acttgagatt gacgcgagag ggtggcgggc gtatggtttt tacgcaagga taaagttgaa
     6061 gtcagaaatt ccgagtcaaa ggatgatgtc ggggcccaac gcaaaacgcc ttaaggttca
     6121 cctcatcaat ccaagtcatg tttcttttgg cactgccgta attacaccgc gctggctgta
     6181 tgtgcttgcg gctgccacgc cggcctcctt cggggatcca attctggtcg atgagactct
     6241 cagccgcgcc aaaccagagc gtattcagcg aggcgacgtc gtgggcatcg gtatccatac
     6301 cgggaacgca ctgcgcggat atgaaatcgg aaaaatggcg cgcgaacgtg gcgcgtatgt
     6361 tgtcttcggc ggaattcacg ccacgcttta ctcggaggaa gcacaacaat tgggcggcgc
     6421 gcacgcggtc gtgaaaggag acggtgacat cgtttgggcg accgttctcg ccgattgcgc
     6481 caagggcgag ccaaaaccca tctatctggg cggccgcatc gaaccgtctg atttcttccc
     6541 ggcccgctgg gacttgcttc ctcccaagag ctacatgtgg gcttccgttc aaaccgtccg
     6601 gggctgccca aagcattgtt ccttctgttc ggtctggcgg acggatgggc agcgccctcg
     6661 tcagcgcacc tcagacgcgg tgatcgaaga gattgtccag ttgcgccgcc tcggctttcg
     6721 atttgtggcc cttgccgacg ataatttcta tccggtcacg ctcaccgatc ttgcgctggc
     6781 aacccgccag ggcgacctgc atcggctcca ggaactcacg gaacttcggg cggaacgctt
     6841 cgaactcatg gaacgcatga cgcaccttcc tcgagacatg gtgttttaca cccagatcac
     6901 tatggaggcg gcggaggaca cgacattcct cgatgcgatg cggcgggcga acatcaaggg
     6961 agcactggtt ggggtggaat ccgtgacgcc ggaagggttg aaagacattt tcaaggaatt
     7021 caatgccgcg ggagccgacc tggtcgagcg cctaaagacg tttcgccgcc acggcgtgca
     7081 tgttctcgga tcgttcatct tcggactgcc cagcgaccgg ccggaaactt tcgaggccac
     7141 ggcggccgtg gccgagcagg cggagatcac cttcgcacaa tttgtgatgt tgacaccgtt
     7201 cccgggaacc gttgattttg cgcgctggga aaaggggttg gagagcgatt ccactcgcat
     7261 cgggggcttc ccccttaccc gccattggct catcccgcag gaccttcgtc caaagctcta
     7321 ctgcgcccac ccggtcatgc cggccgaaga aatccggcgg cgtacacagg gcgtctggga
     7381 ccgcttctat tccctgagac tcatttggaa tcgttcgcga tgtgttaagt cattgccatc
     7441 ccgcctggcc ttcgtcttga tttccaagct ctaccggcag atgtatgcga atactgggat
     7501 tgccacggat agcgcgcgag ccgcacgatc cgcgcgctgg gcgcgctggc ttgcaaagcc
     7561 tacccggcgc cttttttcgg ggcggcccat gccggagctg caggtgcccg cggcagtggg
     7621 cgtttagcgc agcaggcgac tcaccgaatc tctagtacga atatcttccg ggagaagctc
     7681 gagagcgtga cgtcattcca ttccattgtc gcttgcctcc ggcgggtagc ccactcgcga
     7741 gctgggggga ggtcgagggt gcccctcacg tcgatctcat gtgtgcgagt tctcccctaa
     7801 tccgccggca cggcgcgcga cttcgcccat tcccgcagcg cctggatgtc ctccgcgcgc
     7861 gtgacggaaa gaggctgcgt cgcgtgaatc tcaccgagca gaatctccgt ggaaagctgc
     7921 agtttccgcc cgaacgccgt gtacagaccg gcgacgatgg cctgctcgat ctccgcgcca
     7981 ctgaatccct cgctcgcccc gcccagcttc gccagatcaa attgccccgc gtcacgcccg
     8041 cgcttcttca gatgcagcgc aaagagcgat tcgcgaatcg gctcggcggg caaatccaca
     8101 aagaaaatct catcgaagcg ccccttgcgg agcatctccg gcggcagcga gccgatattg
     8161 ttcgacgtcg cggcaaggaa gacgccgctc tcgcgctcct gcatccaggt gagcatcgtc
     8221 gccagcaacc gctgggagag tcccgcgtcg gtctccgcgc ccgccccggc cccggcaaat
     8281 gccttctcga tttcatcaat ccagaggacc atcggcgcga gcttctgcgc cagctccaaa
     8341 gcctttcgca gtctccgctc cgattcgccg atgaacttgt cgtaaagggc cccggcgtcc
     8401 aaccgcgcca gatcgaagcc ccactctccg gcgatggcgc gggcagcaag gcttttcccg
     8461 cagccttgca ccccggtgat gagcactcct ttcggcggct ccaacccaaa agcgcgcccc
     8521 tccgccgtaa gggcgctgcg ccgcttgcca atccattctc gaagatgaac caggccggcc
     8581 acctggccga acgaagcgtc gcgcggcagc acctccagta atgagtcctg ccgcagcgcg
     8641 cgctgctttg catcccggat gtccgtcaac aaccctgcgg cgtcctttcc gcgcgccagc
     8701 aggcacatct gcagcaggcg gcgagcttcc tcccgcggaa ggccgaccag attccttgcg
     8761 agttcctgca tcgttgccgc atccaatccg cccgcgaggt tgctggcgtg cgaagcttct
     8821 gcgagcacct ctctaacgac gggcaggagc tcgtccgcat ccggcagcga catctggtac
     8881 ggcaccgcat ctccggccag ctcctccggc aggtgcagcg atgcgccgga gatgacaatc
     8941 gagcgccgcg ccgcgcggaa tccttccgcc agctcgcgca cccgccggca gatgcggtcc
     9001 tgctcgaggt atcgcgcaaa atccttcaac agaaagatgg cgtcacccgg cacaagcgcg
     9061 agattggcca gggcctgctc cggttgctcg gtgttgtaaa gaggctgtcc gcctcggcgg
     9121 gcgagccccg tcgtgacgct ccacacgtag aagaccacgc ccagttcggt ggcgacatcg
     9181 cagagcaact cttccacgcg cagttcttcg gcggtttcga tggtaatcaa agggtggtgt
     9241 gagttgacaa gcagccggag ctcatccagc ttgtcccgct tgcctgacgc gccagaacgg
     9301 cccgggcgga gcgcagccga gacctgtttc tgaggaagag cctgcggggg cgtcgtcggc
     9361 aactggtttg acaaaggcgg taccctcggc aagtgtcagg aatttccgcg aaagccaaaa
     9421 ggagcaatga atggctcaag gacagcctac caaagttaag aagcgcaaga agtccgttct
     9481 caagcgggcg aggcaatctg gtcaacgggc cggagtcaac cgcgcgaacc tcaccgagta
     9541 ggatttctgt ggagagcggg tgctaccgcc cgaacgaggc gcttccttct cccgcagttg
     9601 cgcagcaagc ttagcgagcg gcaaggccgg cggactggtt ggccacggcc agttggttgc
     9661 aagcgcccag aacctccgaa ataacgatgg gatagagcac cagccaggaa atcatcccca
     9721 cgtacggaat gatacttacg atcacggcgg cgagcaccaa gccccgtgaa atcggcaatg
     9781 gttggccgcg caaccggaac tgaaaattga cgcggtctgc gaggcgcagc cagaaaaaga
     9841 agacccagta gatattgaaa aaagggatga aaaggaaccc gatggctttg ccggcggaag
     9901 ggtcatcgga aaggatctga ggaagcttcc cgtgtttcaa gcccatgtag atgatggtga
     9961 aaatacccag ggtgaagatg tggagggcaa tggccccgcc gacggagaaa gtctccagga
    10021 ggtaattctt cttgtaggcc tcacgctgag caggcgacag gcgatttaca tcgaactgcg
    10081 gttgatacgc gccgcccaac tggaaggcgt ccacgccttg tcctgccgcc ggtggagcgg
    10141 ccgcggcggg ttgcacagca gcgctgggag ccggtgccgg ttgctccgtg agccactggc
    10201 cgcaaaagcg gcacttcact gcatcggcct gaatttcctc cgcgcaaaag cggcatctct
    10261 tcgtggatgg ctggttggcg tccatcggtg tgcagtcctc ctgttgtcca aaggtgagtg
    10321 gacaggacca aaaaaaaaac gacccgcgtt aaatccggcg cgaaagctaa caccgcgcat
    10381 ggctgcaagt caacagcggc tggtttgaca aaggcggcac cctcggctag tgttaaaaat
    10441 tccgcgaaca ccaaaaggag caatgaatgg ctctaggaca ggccaccaag atcaagaagc
    10501 gcaagaaatc agtgctcaag cgagcgaggc aatccgttga acgcgccgca gtcaaccgct
    10561 caaaccgcac gcaggtgcgg acgattatga agcgcatgcg cgctgccatc ggcgccggcg
    10621 acaagaccgc ggcgtccagc ctcttcgcgc cgacgctgtc ggccattgac cgcgcgattt
    10681 ccaagggcgt gctgcacgaa aacacgggaa accgctacaa gtcccgtatc gccattgctt
    10741 tcaatgggtt gcaagcgaag taacggaagt gccgttcgaa acgcatgagg ccttgtttag
    10801 ccctttggcc cctttttgcc ggcattttct tgacaacaaa gtttgacaag taaaattagt
    10861 ttgacaagtg atttgtgggc acaaccgctg ttttgggggc taaaactgcc ctttcgggag
    10921 ccgccaaacg cgcaatcaga aagtccagcg cggctctcgc gtcgcggctt ccgcccttca
    10981 agcggtcatc gcattcctgc aacgcgccga tcccggaaag caattgcgcg cgtgagatgc
    11041 gcggcgcgtt cgaaagcgca atctccgcca tctccggtcg catgccgagc tgccgtgcta
    11101 cctgccacgc atttacggga cctttgatct cttgcgcctc gagcagtttg cggtacatcc
    11161 acgccactgc acccaccacg gcgacaggct cttcgccgtc gcgcaacagg cggtcgagaa
    11221 attccagcgc cgcctcgcgt tgttggccgg cgatcattcc cgccatcgcc cacacggtac
    11281 tcttcttatt cgaaactacg agtgccgcca cgtcctcgag ccggatgcgc cgcgattctc
    11341 ccgcgtgggc agccagtttt tccagctcca tcttcatctg gagcaaatta tcggccacca
    11401 gatcggcgag ctcatcagcc acgcccgaat ccagcttgac gccgagctcc gccgccagac
    11461 gatttgcctc ggcaacggcg ctagcccgtc gcgggaccgc ttcgtccccg aggctcaccg
    11521 ccacgacgag tgtcttctcc gccagaagtt tcgccagccg catgcgctga tcgagcgcgg
    11581 acgcttccag cacgagcgtg gtgaacggcg ccggattcgc cagataggcc tcgatggcct
    11641 tcacgacttc gtcgcgcttt ttatcgccaa gatcctcgat ggcctggaca tcttcgagga
    11701 acacgagctg ttgcggcgaa agcatcggca gggtctgcgc ttgactgagg gccgcaacgg
    11761 tttctccatc cgcggcggaa aatcgggaca cggcccaggc gcgcgcctct tccgggacgt
    11821 atttctcgat gagcagcacg cggcaggcat cccgcagata cagttcgtcg ccaaggagaa
    11881 aaatcgccgg gagcggcttg cctttggcaa gccgcgtgag aagcccttcg gtggtgaccc
    11941 gcgccattag taaccctcgg tgagggcggc gacgaggcgc gaggcgaaat cccgggccag
    12001 acggtcgagg gccgggtctt cctcttcgaa aaagcttttc acgtccgcag tgatttcgta
    12061 ttggttgcga aacagaaaat tattgttgcg gtagagttcg cgcttggtcg caacctcttc
    12121 gagcgcgacc tcgcagcgca cggacaccag catggtcgtg gcgcgcccgg ttgtcgtgtc
    12181 gaacagtagg ggcgtgacct cgatggacgc gactttaccg tgaacgacgg cgtcggcgtt
    12241 ggaagcgtcc gacacgatgc gataacgcga ccgcgccagg aattcatgga cggtggcttc
    12301 ggtcaggcgc tgctcaatgc ggtatcgagt ggtcttgttt tcgaaggccg ggaccgcgat
    12361 cgtgcgaatg gccgccggca gcgaactggt acgcccggcg acgtggtagc cgcatcccgc
    12421 cgcaaaggcg gccgtcaact gcaaggcgca gattgccgtt actttccaga aacgtctcaa
    12481 ctcacccctc ggccggctcc gcgtgcgcgc cgcaaaaaac atttccaaac gcagtccact
    12541 ctgaagactt aaggatcacg ccgtccgcga gaccaggcgc tggcggagga accggataat
    12601 gtgcaggacc actcccgccg ccgcataggt gcacgcaata atcacgagca cgaattccga
    12661 atacttccag atgagcccga caagcaggac gacaaggata atcgtgaggg acggcagctt
    12721 tcgtctccac ggcaagtcct taaaactgta gaaacgaatg gtgctggtca tgagggcgcc
    12781 gagggcagcc gcgagcagga gccaggcgat ggcccagcgc ggattgtgaa gcggtatctt
    12841 gaaaccgtga acgagcgatg caatcacacc cgcag
//
LOCUS       DASYVO010000430        59283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_40458, whole genome shotgun sequence.
ACCESSION   DASYVO010000430 DASYVO010000000
VERSION     DASYVO010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 59283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59283
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40458"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VGR03_14600"
     CDS             <1..741
                     /locus_tag="VGR03_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995558.1"
                     /translation="LGPWIIRNATLAAYAGFTPAPDYILYFYSAAAVKAKAEHKKFSQ
                     VQEELGYGEWGYVNNVLYLQRHPEQRTWSQADIFRFEGAEARRTIFQDPRLYSLIHLK
                     GCTIVILDPGVTDLLKTVRLYPEDGGLLSRVIDQGPFRATLWLIRNYPFTMVMLFLLG
                     TQLIVYYILALAGLRRLPMHARVFFAWTALYFVLVSGGPAGLARYRVPIMPLICISAG
                     VAVAYWTAKTAPQKDPSALRDASAEFSR"
     gene            complement(812..1477)
                     /locus_tag="VGR03_14605"
     CDS             complement(812..1477)
                     /locus_tag="VGR03_14605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1995559.1"
                     /translation="MADVNAQLEKIYHRRFDADLEYRNGVWRVLTREFFSRYIRPSDH
                     ILDLGCGYGQFINHIQCAHRYAMDLNPASKNVLQNEVKLFAQDCSSRWPLESGSLDLV
                     FTSNFFEHLPTKGHLMLTLQEAHRCLRSGGRLIAMGPNVKHLPGAYWDFFDHHIPLTE
                     LALQEALEVAGFRCLEVISRFLPFTMVNAPKYPLRLVSLYLKLPWLWRWFGRQFLLIV
                     QKP"
     gene            complement(1464..2255)
                     /locus_tag="VGR03_14610"
     CDS             complement(1464..2255)
                     /locus_tag="VGR03_14610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1995560.1"
                     /translation="MKPLSILIPVYNEGANFRQLWGEIARLVKSGFTAYVIYDFDEDD
                     TVPVVKQVVANGEQRLRAVKNRRGRGVVPAIMTGFDVAADGAVLVVMADLSDDLAQVD
                     RMLELYNQGYDVVVGSRYMKGGRLIGGPFLKQMLSRLAGVSLFWLRRLPTHDATNAFK
                     IYDRDMVRSFTVESRGGFELNLELTVKAFLGNYRITEVPATWRDRTRGQSRFRTWKWL
                     PLYLRWYFYAFRRKSAKQPDLSLRRPGPEKSSAAVQEATHDNGRR"
     gene            complement(2264..3286)
                     /locus_tag="VGR03_14615"
     CDS             complement(2264..3286)
                     /locus_tag="VGR03_14615"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1995561.1"
                     /translation="MKTLVTGAAGFICGYLIEELLGAGHEVIGLDNFSKYGRAEKSYD
                     AHPRYKFVEGDAKNVALLKELVGECDHFVAAAAMIGGITYFHEFAYDLLAENERITAA
                     SFDAAVWAHRNRKLRKITVLSSSMVFESTSEFPTPEGAERRCPPPRSTYGFQKLATEF
                     FVQGAHEQYQLPYTIARPFNCVGIGEKRALCDREIMSGNVKLAMSHVVPDLVQKVLKG
                     QDPLRILGAGNQARHYTYGGDLARGIRLCIEHPAAMNEDFNLSTPVRTTVLELGELIW
                     RKIHGSKKPFRYVSDPAYEYDVQERSPATDKAARLLGFQATTSLGEILDEVIPWIAEQ
                     IEVGKI"
     gene            complement(3283..4542)
                     /locus_tag="VGR03_14620"
     CDS             complement(3283..4542)
                     /locus_tag="VGR03_14620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEV1995562.1"
                     /translation="MQQEKSESTNPMPTPGKIVIIGGCGHVGLPLGIVFANCGISVTL
                     LDIDHQKIAAVNSGHMPFMEKSAEELLRKVVSKNLLATADNSCLRDADVAIAVVGTPV
                     DEHLNPTVTELYRNIDSTLELLPDNSLLVLRSTVYPGVTKLVYDRVQSRGRRIHIAFC
                     PERIAEGKAIEEIRSLPQIISAFEPEALDRARGLFAIINGELIELTPLEAELAKLFTN
                     SWRYLNFAISNQFYLLAESYGVDFYRIHDAATRRYPRMGSFARAGFAAGPCLLKDTLQ
                     LSAFSGNNFFMGHAAMLVNEGLPNFIVSQLRKSGLHGKVVAILGMAFKGDSDDKRESL
                     AYKLRKLLMLEAREVLCTDPYIKDKDFVPLDEAIQRADIIILGAPHSMYLSLPVPQGK
                     HIVDVWGFWQERETAGASALTARHKSQ"
     gene            complement(4743..5651)
                     /locus_tag="VGR03_14625"
     CDS             complement(4743..5651)
                     /locus_tag="VGR03_14625"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEV1995563.1"
                     /translation="MSKYRVVMLLVAFAVGTTAAYGQASRTWVSGVGDDANPCSRTAP
                     CKTFAGAISKTAVGGEIDALDPGGFGAVTITKAITLDGGGGQVASVLVTGTSGIIVAA
                     PAGAVVILRNLRINGISGGINGIQFNSGKALIIENCNIFGFTQRGISIQTSAASQVFV
                     TGTSSNNNSDSGLKSINTGATTQVTIANSNFLGNGTFGIFAGDFTDMSVFHSTASGSV
                     IGVIAQAAAGTATGRVSETTVANNTIGMQAGGAGAASLLQLGHNYIFGSATGLLAGAN
                     GTINTGGENLNTGAGAPTGAGSIQIQ"
     gene            complement(6075..6695)
                     /locus_tag="VGR03_14630"
     CDS             complement(6075..6695)
                     /locus_tag="VGR03_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995564.1"
                     /translation="MKRLFAFAALLSFPLLLMAQAKSIRFTQDGEFASVSQFTAPFTV
                     VSLQVSRGATTAAGATAFLQFSSVSEPADFSSITIINIFGPIPPEDFTGVNTHNLVLN
                     VDTSTLDPAIFTTETCTIDFTTFITTCGPGPAGLIHLEFRENGAQRSIVDLKQTTYSG
                     PVTTHVHQKSDNSTADVQGSIFGSSFTGAPANVGVNHQSTLQITHD"
     gene            complement(6867..7904)
                     /gene="rfbB"
                     /locus_tag="VGR03_14635"
     CDS             complement(6867..7904)
                     /gene="rfbB"
                     /locus_tag="VGR03_14635"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEV1995565.1"
                     /translation="MKVLVTGGAGFIGSNFVHYTLRNHPSDQVVILDKLTYAGNLRNL
                     EVPLADSRVKFVRMDVCDPAVASVVKGCDAVVHFAAESHVDRSIEDASPFVRSNVEGT
                     WRMVEACRRSDVGRFVHVSTDEVYGSLGPEGRSDESSSLAPTSPYAASKAASDLLVLS
                     AIKTHGFPGIITRCSNNYGPFQFPEKFIPLMICQAMANQPLPVYGDGRNVRDWIHVAD
                     HCRALDLILRKGKEGEVYNIGGGCELENLTVARAILKILDRPESLLQFVADRPAHDRR
                     YALDCAKLKRKLGWAPAWDFARGLVETIRWYKENTAWLQEARSGEYQKYFERHYLRRE
                     ETFAASGKPGG"
     gene            complement(7901..8665)
                     /locus_tag="VGR03_14640"
     CDS             complement(7901..8665)
                     /locus_tag="VGR03_14640"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEV1995566.1"
                     /translation="MKGVVLAGGTGSRLHPLTRVTNKHLLPVYDKPMIYYPLRTLVDA
                     GIEDILIVTGGNSAGDFLRLLRNGKDFGLQQLNFAYQEGEGGIADALRLAEYFAGKDN
                     VCVILGDNIIERSMRAARQNFEKQGRGAKIVLKEVPDPERFGVPVFDNGKIVKIEEKP
                     KIAGSSYAVIGIYFYDSTVFDRIRSLKPSGRQEYEITDVNNSYLADGQLTYDTLDGWW
                     TDAGTFESLWRASSLVREKTVGDAETTLASRGGSQS"
     gene            complement(8804..10057)
                     /locus_tag="VGR03_14645"
     CDS             complement(8804..10057)
                     /locus_tag="VGR03_14645"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEV1995567.1"
                     /translation="MAEQEQKEIELPVSEEVSLLQMSEGPEKNHFLEPFILLAKHKFF
                     ILYFVVGAAVLSVAVSLLLPVYYTANAKILPPQQSQSISSAMLNQLGQFGPLAGLAGQ
                     GLGVRNASDLYVAMLRSRTVADRMIDRFSLMNVYHKMVHMDARRRLENLTEISAGKDG
                     IISVSVDDRSPTRAAEMANAYIDELEKLTRTLAVTDAGKRRLFFEREVKTANEELVVA
                     ELSLKQTEESTGIIQLDSQSKVMLQAYADLRAQVGIKEVQVQSMRSFATPENPDLIRA
                     EQELSALHAQLRRFERGQGGRSVADIALEKVPGAGLEYIRKLREVKYRETLFELLAKQ
                     YEAARIDEAKDASVIQELDKAVPPEKKSWPLRAVIVVVVTILALLLAILVVFAMEALE
                     EAKKDAQFATRLRLFQSYLRVGHKS"
     gene            complement(10153..12348)
                     /locus_tag="VGR03_14650"
     CDS             complement(10153..12348)
                     /locus_tag="VGR03_14650"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEV1995568.1"
                     /translation="MGIKLPVFGQNLFQNVPSTFAPLDRVEVPADYIIGPGDEVLIRA
                     WGQIDVDWRAVVDRAGAIYIPKIGTLSIAGVKYDDLHDYLQAHIGRIFKNFQLSVSMG
                     RLRSIQVFVVGQVRRPGLYTISSLSSLVDALFASGGPSKRGSMRRIQLKRAGKIVTTF
                     DLYDLIVNGDKTKDAKLMPGDVIYVPSVGPLVALAGSVNIPAVFELRKEGTALEEVIG
                     YAGGLTNTAAGEHAVVERIGDHHIRQADEFPLNKEGLARELHDGDVVRFLRLSSKFEN
                     AVILRGNVAVPGRYPWHQSMHVKDLIPSRDFLITEEFWKRQNKLAIDPDGASFKLREE
                     LERERDREFQPGDKDTSPANQPGVQNQQTEQPPNSSQNRDRDMQSDRRSSTRGDNLAE
                     RVGRTEAQRIKQEELKNEVKRSGAEINWEYAVIQRMNPEDLTTHLLPFNLGKAIVGDE
                     AQNLTLQPGDVITIFSQSDMQVPTGQQSKFVRLEGEFRSAGVYQAEPGESLRHVITRV
                     GGLTPQAYLYGAEFTRESVREDQQRRLDQYVNELEKAIERNAGSQRNLSGEEVIAERQ
                     AAESQRRLLDKLRQLKATGRIVLELRPNANDLQAFPDLVLEDGDRLLVPFRPATVNVI
                     GSVYNTNAFIYKQGKTFGDYLRVAGGATRNGDRGRSFIIRADGTTVSSQGHNGALLRS
                     FNMVRLMPGDTIVVPERLDKGVTLRGFKDWTQIIGQFVIGAAAAKVLFP"
     gene            13561..15837
                     /locus_tag="VGR03_14655"
     CDS             13561..15837
                     /locus_tag="VGR03_14655"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HEV1995569.1"
                     /translation="MTIKSVVLNLPKDQLAIWTSPFHARRRDLTWLLPLAVTSGLLIG
                     SDSHSMAREQSNAVAVHRSTQISNYGVAALLSVPALTYLAGQWNGSSRARETGLLAGE
                     AAVNSFIVNEVVKLTVGRERPTLTDGRGRFFTQFGNSSSFPSEHAMLAWSVASVVAHE
                     YPGPLSQLVVYAAATAVSLSRVTARQHFPSDVVVGAAMGWLIGRQMYRAHSTNRDDED
                     YGASEHDPESSGGPPGSIFVPLDSWIYPALKRLASLGYIPTQFAAMQPWTKRECVRQL
                     GEAEDLAQDLPPEADVRQLINSLKKELGAGSDYDHSAQIESVYTRYMNISGVPLRDTY
                     HFGQTISNDFGRPYDQGNNFIAGATASAVAGRFFFYVQGEYEHAPGRPALSATQQNLV
                     NSLDGNVGPLPNPIGPQPAPVAQIDRFYPLDMYAGVQLGGYAVTFGKQSLWLGPGESP
                     PLMLSDNADPMYMLRLAHTSPFYLPWIFRYLGPVEDEYLFSKLSGHQFPARPFFNLQK
                     ISFHPTRNLEIGFTRASLWAGVGHPFTLHSLERNFLALGDTPAGPFNSPNDIGDRKSG
                     FDFSYRLPGLRNWLTIYCDSYSDDDPSPIANPRRAAVSPGVYLSRVPGIPKLDLRLEA
                     VSTQSLTATDRSGQFLYWNLRYHDSNTNKGFLFGNSTGRDGRALQGWSTYHFAPGKTL
                     EFSYRQIKVGNGFLPGGGTQSDAVTKFAWRLNPEWTVDSFLQYERWLIPSLLPTEQRN
                     FSTSIQVTFHPHWQIHQR"
     gene            complement(15891..17870)
                     /gene="asnB"
                     /locus_tag="VGR03_14660"
     CDS             complement(15891..17870)
                     /gene="asnB"
                     /locus_tag="VGR03_14660"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEV1995570.1"
                     /translation="MCGIAGFWQSKRGPEHPLETLNRMGAALVHRGPDDSGTFHDGST
                     GIGLAFRRLSILDLSPEGHQPMFSCSGRYVIVFNGEVYNFQEIRAELGTRLWRGHSDT
                     EVMLEAFERWGVDCAVRRFVGMFAFALWDRQERRLYLVRDRLGIKPLYYGGIGGDFVF
                     ASELKAIRQYPEFSPTIDRNSLALYMRHSYVPAPHSIYVGLQKLQPGCVLTLSEAGEA
                     PVLQPFWSASDVARESLQSPFEGSDAEAVELLHQKLLEAVRLRMIADVPLGAFLSGGI
                     DSSTVVALMQAQSNRPVKTFTIGFHEEGYNEARDARRVAEHLGTDHTELYLTPRDALD
                     VVPLLPAMYDEPFADSSQIPTYLISKLARGAVTVSLSGDGGDELFGGYNRYLLNKRIW
                     NSVGRLPKPFRTAIAGLIRAVPPATLDHALRLLRPLLPRSMRMSAQGDKAHKFAGLLS
                     ATSPEELYSRMLSHWQYPSKIVLNSHVANRIVASVASSAWLPSMEEVMMLTDLLDYLP
                     GDILTKVDRASMAVSLEARVPLLDHRVVEFAWKLPLHFKIRNGTGKWILREVLYKYVP
                     AERVERPKMGFGVPIDSWLRGPLRNWAEDLLSSTSLTQHGLLNPDIVREKWREHLSGA
                     GNWQYSLWNVLVFQEWFFNAFLSSKDRIDCQPIRQ"
     gene            complement(18421..19194)
                     /locus_tag="VGR03_14665"
     CDS             complement(18421..19194)
                     /locus_tag="VGR03_14665"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1995571.1"
                     /translation="MKPNGESREPFQLQSSSLYLVSPWCESAGKRVFDILGAILLLIL
                     LLPLMLAVALAVKLTSQGPILFRQRRPGKNGREFTILKFRTMINGNHDTGPPMTRAAD
                     PRVTRSGRFLRRWKLDELPQLFNVLCGQMSFVGPRPLPTGHWQQPSIQKEAAYVLSVR
                     PGITSPTTLIFRNEEELLAALSFEEVEEVYMRAIMPLKAQMDLQYLQSAGFTSDLRII
                     SITAFRVFSRQDGDNDLIIRHHLPVLKQEEFLPFGKHAD"
     gene            19841..20308
                     /locus_tag="VGR03_14670"
     CDS             19841..20308
                     /locus_tag="VGR03_14670"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV1995572.1"
                     /translation="MSAEFMRRAIALALENVRSLGGGPFAAIVVKEGRVIAEGANRVT
                     ALNDPTAHAEVVAIREACRILRDFNLTGCDLYATCEPCPMCLGAIYWARPARVFYAGT
                     AADATAAGFDDALIYQELNRAPAARRIPMSQLLREESLAIFSAWKDKADKIAY"
     gene            complement(20309..20842)
                     /locus_tag="VGR03_14675"
     CDS             complement(20309..20842)
                     /locus_tag="VGR03_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV1995573.1"
                     /translation="MKKGAAKAGRTVIRKQTRRDDVALRKQLVHLLKGGGAHVHFEDA
                     LKDFPESKRGVLVPGLPHTAWQLLEHARIAQWDILEFSRNAKHVSPDFPGGYWPKTPL
                     PPDNAAWEKSVRTFRKDLQGLVRLVESPRTDLFAKIPHGNGQTLLGESLLAADHNSYH
                     LGQLVDLRRALGAWPEA"
     gene            complement(20839..21867)
                     /locus_tag="VGR03_14680"
     CDS             complement(20839..21867)
                     /locus_tag="VGR03_14680"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1995574.1"
                     /translation="MTASPAAPQPTPERIFNTLNAYQQTAAMKTAIELNIFTVIGEGA
                     NQPAAIAARIGAAERGVRILCDYLAILGFLIKEQNRYALTAESALFLDRRSPASMTSL
                     IGFLGREELTGRFQKLTEAVRKGGCATQGGDNTEPQAELWVAFARSMAPLMVPAAAFL
                     AELLEARTGSPWKVLDIAAGHGMFGITLARANPRAQIVALDWEPVLEVARENARAAGL
                     ADRYTLRPGSAFETEMGEGYDIVLLTNFLHHFDPETCEKVLRRVHAALRPGGRAVTLE
                     FIPNEDRVSPPTPAAFSLIMLAETDAGDAYTASELECMFENTGFRNSTLHSVPGMPQR
                     VMVSEKAS"
     gene            complement(22181..22483)
                     /locus_tag="VGR03_14685"
     CDS             complement(22181..22483)
                     /locus_tag="VGR03_14685"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEV1995575.1"
                     /translation="MEYHDRVLKCSDCDAEFVFTSGEQMFFADKGFKNEPKRCKTCKA
                     KRAEHSGGPAPARTETKTNCSQCGKETTVPFRPTQGRPVFCRECFQQRRAMGAQPS"
     gene            complement(22690..22887)
                     /gene="rpsU"
                     /locus_tag="VGR03_14690"
     CDS             complement(22690..22887)
                     /gene="rpsU"
                     /locus_tag="VGR03_14690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV1995576.1"
                     /translation="MAEVIIQETESLENALRRFKRKVQQEDIIKEIKKHSFYMKPGEK
                     RRAKEALSRKRLRKKQRREQD"
     gene            23229..24467
                     /gene="glgC"
                     /locus_tag="VGR03_14695"
     CDS             23229..24467
                     /gene="glgC"
                     /locus_tag="VGR03_14695"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002023.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV1995577.1"
                     /translation="MTDMRDAIGVLLAGGQGERLWPLTRDRAKPAVPFGGVYRIIDVT
                     LSNCLNSDLRRVFVLTQYKALSLNRHIRRGWSALAGLGDYFEILTPQMRVSQHWYQGT
                     ADAVYQNIYSIGSERSKFVFILSGDHIYKMNYRKMLDQHIESGADVTVATVETSLEEA
                     AGRFGVMETDEHWRITGFEEKPDLPRLARQNADKCNGSMGVYIFNTQLLIPILLADAE
                     DAKSSHDFGKDILPRIIHKHRVFAYNFEDENRRGGQYWRDVGTIDAYYEANMDLVSVS
                     PVFNLYDKDWPVRTWQQQYPPAKFVFADADRMGVALDSIVAGGSIISGSRVVHSVLGY
                     DVRVNSYSEISHSIIYNHVAVGRHSRIRNAIIDRHVTLPEHTEIGYDVEADRRRFHVT
                     ESGIVVVVRQESLIEEPESA"
     gene            24548..25864
                     /gene="eno"
                     /locus_tag="VGR03_14700"
     CDS             24548..25864
                     /gene="eno"
                     /locus_tag="VGR03_14700"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV1995578.1"
                     /translation="MSTRIARVHARQILDSRGNPTVEADVILDGGALGRAAVPSGAST
                     GEHEALELRDGDKSRYAGKGILKAVGNVNGEIAKGLAGSDAADQRALDRKMIALDGTP
                     TKSRLGANAILAVSMAAARAAAAALGLPLYKYLSRYSTDASANLLPVPMMNILNGGAH
                     ADNSVDFQEFMVMPVGAPSFSEGLRWGVEVFHALKAALKKRGYSTAVGDEGGFAPNCK
                     SNEEAIQVILEAITRAGYKPGEQVAIALDPASSEFYDKAAAKYVFKKSDKSAHSSEEM
                     AAHWARWAEEYPIVSIEDGMAEDDWAGWKLLTQKIGAKSSPKRIQLVGDDIFVTNTDI
                     FARGIREGIANAILIKLNQIGTVTETIDAIEMARKAGYNSIISHRSGETEDTFIADLA
                     VATAAGQIKTGSASRTDRIAKYNQLLRIEEQLGGSARFPGRSVFSS"
     gene            complement(25898..26398)
                     /locus_tag="VGR03_14705"
     CDS             complement(25898..26398)
                     /locus_tag="VGR03_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263657635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(26303..26305),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HEV1995579.1"
                     /translation="MVLPDADSREVRLGSLWASQPAVLVFLRHYGUIFCREHVAQLRE
                     HEQAFQEKGATLAAIGLGDRHYARAFREDAGITFPLLIDEQRKAYRVAELRSANLLHL
                     LRGDNAAARKRARAAGHHQHKLGPNPFQLGGSFVFSPGNVDRFVHVSQTFGDNASPAA
                     LLAALP"
     gene            complement(26538..26897)
                     /locus_tag="VGR03_14710"
     CDS             complement(26538..26897)
                     /locus_tag="VGR03_14710"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV1995580.1"
                     /translation="MRAPSEETILKKLRRACLQLPGATETVTFGHPTFQIEKKTFAVL
                     EEYKGELGICVQVGKLMQGIFLKDARFFRTPYIGKHGWVTLRVYAAPLDWKEVSALVE
                     GSYREVAPAKKRMRSSG"
     gene            complement(27061..30648)
                     /locus_tag="VGR03_14715"
     CDS             complement(27061..30648)
                     /locus_tag="VGR03_14715"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV1995581.1"
                     /translation="MSMKLKGIAVFVALTLALVFCNASWGQVLKGSISGTVVDQQGGV
                     VTGAQVKATHAETGAVYTTTSDSSGLFRFNLMPVGTYKVEIQAKGFKTTVAKNESVGA
                     GVDTNLGSLKLTVGEVTDFLEVTAGAPLIETSTAQVTNTFGQVELNTFAGIQENQGLD
                     NLALFTPGVSSVRDNSFSNTNGGLGFSVNGLRGRNNDQEIDGQNNNDNSVGGPGLFVT
                     DAEFVQQYVLVTNNFGPEYGRNAGSVVNIITKSGTNAWHGNLFGTENNSILNSLNNFD
                     KNKDICDPNCKTKPNRLNDEFGGFTIGGPMIKNKAFIFGGFDEEILGQNTTLTSSAFT
                     PTPAGLAALAACFPASQSLQALLKFGPFAISGGNPKVVGTPVALVGTCPAATFAGVSR
                     TVAQPFHGFDFILRNDWQVRGDTITARYIFNRGNSFNTDAFGTGAAGYVANVPALSQV
                     MLLGWTHNLTAHMVNEARISFGRLNVEFGGNSIGNTVPNAGQIDQALANVTFNSSTLL
                     GFGPATNAPQGRIVNTWQAQDNWNYVLGKHSLKAGVNFTYQRSPNTFLPNLNGQYRFG
                     SWTSFLNNTPNRIRIANGNPTLDFREYDSFIFAGDDWKIGQHLTLNLGLTWTYYGQPA
                     NLFNDITTAREASATPFWASTEPLGTNNLGDTFTSPGAAIPVSARTNPLIPSPKKSFG
                     PSIGFAYSPQWGGFLTGHGKTVIRGGYRLLYDPPFYNIYLNNSTAAPFSFLNTVPSPG
                     THPLPGVPTGPNVRASLASSIQKGVFDPREFSQTQITPTFGPDRTHTWSLGIEREIAK
                     NAVIEARYVGNHAFGLFQTLDGNPFIADLKADFPSLVPAGLTPCPASSALFTRTGNSG
                     SFGNDPVGRINCNFGVVRLRGNSGFSNYQGLQVEFRANNLFKQLTVRSAYTYSKTLDN
                     VSEIFSTFGGGNTIFFAQNPANQVNGKGEYSFSGLDYPHQWSILFSEQLPFFKEQHGL
                     LGHVLGGWGLSGNYIIASGQRYNPAQFGDAAFSAAGDYYDFGYILGFAGVDTARPFLG
                     NLSAPATSVGAFCGDELSAATCTTAFGPSGVTQLISLTTLQGPTGALVATTKDKVRFI
                     INAFESQTIFGTPFGNAPRNTVQDAPTNIGNFSVYKSVKLSERVTFQFHATFQNVFNH
                     SNYASVDPFLDDAGLTGAFNGFGDPTQTPSVNNGAPPTRRIFFGGKVSF"
     gene            31034..32329
                     /locus_tag="VGR03_14720"
     CDS             31034..32329
                     /locus_tag="VGR03_14720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEV1995582.1"
                     /translation="MSSPARSERSDERSPAPLELQWVRAQFPSLQLIVNGQPAAFLDG
                     PAGTQVPKQVMDAIQDYLMRSNANTCGAFVTSRRSDALIAGARAAMADLLNCAAEEVS
                     FGPNMTTITFAFARAFGRGLQRGDEIVVTTLDHDANVAPWRALEENGAVIRQVDIRAE
                     DCTLDLDDLKRKINSKTKLVAVGYASNAVGTINPVAEIVKFAHAAGALAFLDAVHYAP
                     HGLIDVRSLDCDFLACSPYKFFGPHMGVLYGKKEQMTRLRPYKVRPAPDQIPDRWETG
                     TQVHEGLAGVAAAVDYLAELGRRCDPGARSRRSALQAAYRATRAHEATLVARLIEGLL
                     AIPGLRFYGITDPKRFSERCSTVAVRLANHTPLEIAKFLGERGIFTWDGNYYALNLTE
                     RLGVESSGGLLRIGLVHYNTAEEVERLLCALHEFVQQHH"
     gene            32388..32843
                     /locus_tag="VGR03_14725"
     CDS             32388..32843
                     /locus_tag="VGR03_14725"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEV1995583.1"
                     /translation="MSEEGVVASAGAELSQDEKAYAGLAHALMISTWWIGPLIIYLIR
                     RESRFVSFHALQALFWQIILTVLHFTGMIVFFVAVFSTLLSVPPGQTPQAPQFPAGFL
                     IAFPLLWLTYMSGFVISMTLGIIYCLKAMRGQWAGYPLIGRWARRIVKV"
     gene            32845..34395
                     /gene="gpmI"
                     /locus_tag="VGR03_14730"
     CDS             32845..34395
                     /gene="gpmI"
                     /locus_tag="VGR03_14730"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002139169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEV1995584.1"
                     /translation="MAKRPRPIVLTVLDGWGYRAETKGNAIALARKPTYDALLKKFPN
                     TLIHTSGPYVGLPEGQMGNSEVGHLNIGAGRIVRMDITRIDGLISTGEIFKNKLFLQA
                     MERGRSRRLHLMGLLSDGGVHSHIHHLFALLEMAKREKVERVFVHCFMDGRDTPPDSG
                     RDYLRQLQQKMRELGCGEIATVVGRYYAMDRDNRWERVEKAYRAMVHGETETHSDDPI
                     AAIQRSYEQGLGDEFVEPIGIQRGSDPEPVGLIRDDDAVIFYNFRADRARQMTRALLD
                     PAFNGFTDTKRPKNLFFVAMTQYEKTWPWLQYILGPEKLEHILAQVFVALNYRNLRCA
                     ETEKYAHVTYFFNGGVEKPYAGEERILVPSPKVATYDLMPEMSAAGITDTVVKAIESG
                     SFDAIIMNYANADMVGHSGKLEAAIKAVEAVDAGLGRIYQALKPRGGAWIVTADHGNA
                     ETMIDPVTGGPHTYHTTNPVPFLLVSEDEKLRLRAGGSLRDIAPTLLDVLGDPQPAEM
                     TGQDLRLR"
     gene            34407..34634
                     /locus_tag="VGR03_14735"
     CDS             34407..34634
                     /locus_tag="VGR03_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995585.1"
                     /translation="MLAHLKTRKDHDASFDPRSLVGAAYVRVTHSPVALLIAAGLAII
                     LYVGVPELRLVLLAIPVGGALVGLLLRLKHR"
     gene            34640..35647
                     /locus_tag="VGR03_14740"
     CDS             34640..35647
                     /locus_tag="VGR03_14740"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEV1995586.1"
                     /translation="MNRTKPAETKLVICVWHPFTEWRPKPILAETLRRRWPEMRVVHL
                     PDYERLPQELADTDIFVGYSLRPRQLQEGRKLKWIHSTAAGVAQLMYPELRESGLLVT
                     NPSGIFSIPMAEHTLGLILALARNFPDSVRYQDTCRWAQQELWDKPQHLTELNGQLLL
                     IVGMGSIGRELARLVRALDLRVWGVSRSGKGDAALAERMLPASQLHAALPEADYVVIA
                     APETPETKHLIGAAELARMKPTARLINVARGSLLDEAALVHALETGALAGAAIDVAAA
                     EPLPPDSPLWRAPNLIITPHTSALSDRLWERETALLVELLERWFDGREMTNRVDLARG
                     Y"
     gene            complement(35680..36756)
                     /locus_tag="VGR03_14745"
     CDS             complement(35680..36756)
                     /locus_tag="VGR03_14745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV1995587.1"
                     /translation="MRAAAWGILIGILLAALVLPLGEMPVAAGAQQEAPKPPAQQPQQ
                     QGEVAISVEIPVVTVDVVATTQHGDILGGLKKENFRIYEDGVPQTITNFSPSDSPITL
                     VMLMEFSKVYGGIFAAYAKYWADPFFHRLNPKDWVALVIFDMRPKLEVDFTQNKEEVR
                     QAIYRLYFPGFSESCIYDALLDTLDKLKDVRGKKSILILASGIDTFSKHTLDQTLKQL
                     KQTDVTIFAVGVGKGYVDALDARGGLGAVGRLGYYQAQNQLHAFAEMTGGHSWFPQFD
                     GEIPGIYQDVVAYLRNQYSLAYTPTNRTRDGKFRKIKVELVEPDGSLLDVRDQKQKEK
                     NQKLPKIVVYARQGYIAAKDNAGD"
     gene            36991..38430
                     /locus_tag="VGR03_14750"
     CDS             36991..38430
                     /locus_tag="VGR03_14750"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1995588.1"
                     /translation="MFSLFLAVAAAFPVSAQQTPAPIAPPAAAPIEDTTALAREAVRW
                     LTDLIRINTTNPPGNEQAAAKYIAGILEKEGVTPELLEVAPGRSAVVARLRSAALADP
                     SRALLLVGHLDVVGVERARWTVDPFAAVVKDGYLYGRGSLDDKGMLAANLAVFIALKR
                     ANARLNRDVIFLATCDEEREGNAPDAGIKTLIAKYWDKFAAGFALNEGGYVVVKNGKV
                     LYAAVQAIEKVEANVSVVARGPSGHASQPGKDNAVVHLAAAIAKIGTYSAPVRPTAIV
                     RRFFEELAKIEDPEMGKWMRALETSDRGEHAQRVISETNPLWNSMLRDSIAPTMLSAG
                     AFPSAIPSEARAVLNIRLVPGDSVNALVAELTKLVNDPQVRFEVMPGAGFPAPPSALD
                     SELYRAIEKASQEQFPGIAVLPLQGTGTTDSAQLRLHRVEAYGLTPFPMTEEDLRRSH
                     ADDERIPLSAFALGVQFLYRIVSEFAIAK"
     gene            38488..39912
                     /gene="pyk"
                     /locus_tag="VGR03_14755"
     CDS             38488..39912
                     /gene="pyk"
                     /locus_tag="VGR03_14755"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV1995589.1"
                     /translation="MLFRRSKIVCTIGPASRTPRVIERMMRAGLDVARLNFSHGSHAD
                     HAQSIALLRETSVRLRKPIAILADLQGPKIRTGALAGGKPVTLRAGQRFVITTARVLG
                     DSTRVGTTFTPLPREVHPGDRVLLADGLIELRVRQVRGREVACEVVNGGELGEHKGVN
                     LPGIKLSVPALTKKDRADLAFALAHGVNYIAVSFVRRREDVQLAKAAIRRAGKDTPVI
                     AKLEKPEAIENLDAILMAADGVMVARGDLGVEMSPERVPVMQKEIIARAREFRRPVIT
                     ATQMLESMTQNPRPTRAEASDVANAIFDGSDAVMLSAETAAGKYPVEAVAMMARIIEE
                     AEESVHEFPRPAPSDQLKVAETVAELACHASRELHMKAIAVFTHSGFTAQLVSRYRPQ
                     TPIIAFSPEAETRRRLALIWGVLPRSISNVRKIDGLAAIAEKRLLEERLVRRGDIIGI
                     LAGTPMGIRGTTNFMKFHVIGGGA"
     gene            complement(39949..40701)
                     /locus_tag="VGR03_14760"
     CDS             complement(39949..40701)
                     /locus_tag="VGR03_14760"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV1995590.1"
                     /translation="MSFNFYITAGVRLLLIANFSVFLFQVILNHYWGASAHHRLLMWF
                     GLVPAGVVPGLRIWQPFTYLFFHEVGTIWHIFFNMLLLWMFGKELELVWGRNRFLQYY
                     FLTGVGAGLINVIVKTVPVLFGAGFSYVPTIGASGAIFGILIANAILFPDREVMFFPI
                     PVRIRMRTAVAVMAAFTFLGTFGLGDDKVSHVCHLGGMLVGWLYLRRSSFLYRLRNGL
                     SDWKYQRNRKRFEVFMKDHKDEPPPRPDRWIN"
     gene            complement(40934..43135)
                     /locus_tag="VGR03_14765"
     CDS             complement(40934..43135)
                     /locus_tag="VGR03_14765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEV1995591.1"
                     /translation="MSTRPWSSTAPPGEFRTYVPTEESPIEFSLRAVLLGALFGLLFG
                     AVTVYVGLRAGLTVSASIPISVLSISILRAFGRSTILENNIVQTTGSAGESLAAGVMF
                     TLPALIFLGFGSYFTYLRIFPLALLGGWLGVLFMIPLRRQLIVKEHGSLTFPEGTACA
                     DVLVAGERGGSFAGRVFWGLGLGGVYTFLMNTAQAWTGQPEHRPVWFPGASFRAAITS
                     EYLGVGYIIGPKVGGLLFAGGVVSWLLVMPAIRFFGQFSGNTPIYPATIPIPLMSPDD
                     MWRSYIRPMGAGAVAAAGLITLLRTLPTIVSALRSGMKDLRAEGKSGATGTSRIDRDM
                     PMRTVVLGSLAIIVMMWLLLTFKPIPGAQTSLLSNIFAAVFVVVFGFLFVTVASRISG
                     LLGNSSNPISGMTIATLMATCAVFLVSHWTAPNYAVLALTIGGVVCIAAAIAGATSQD
                     LKTGYLVGSTPVWQQWGLMVGVTVSTIAVGLTLSLMNKGLEKYTPAQIPLNVQSLPAG
                     VKIEKDRFEYKGKTYELVNALGSGEIADGEYLYDPATKQIEVQWIQGIGSEKAPAPQA
                     RLMATVISGILNQRLPWRLVLMGVALVFAVELLGVRSLAFAVGSYLSIATTAAIFAGG
                     LVRWLAERGSKKEHSESEASPGALYSSGLIAAGGIFGLLAIVINLLQDPDISAHLPHW
                     VISIFHLPLRADLFAFGPKILGSLAQSNLFGMVMFLFLAGSLLFFAKKKLN"
     gene            43455..45413
                     /locus_tag="VGR03_14770"
     CDS             43455..45413
                     /locus_tag="VGR03_14770"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEV1995592.1"
                     /translation="MNGCRSARLYLVLASVCWLLGAPRTGAGDDWPPVSQEELKMTSE
                     PQAPGAPAIYLFRKVDRDDQNAHEDNYARIKILTEEGRKYGNVELPFIKGQQSVRGIK
                     ARTIRPDGTVVNFDGKVYEQTVIKAKGLKFLAKTFTMPEVQVGSILEYRYVYDWEGYI
                     FIDSRWILSEELFTKHASFSLKPYLRYALRWSWPTGLPAGTVPPKDEHGTIRLDSQNI
                     PAFQFEDYMPPENELKFRVDFVYLPAEDSPEKDPDKFWKREGKKLYDRVEAFAGKRKA
                     MEQAVTQIVEPNDPPEVKLQKIYARVQQVRNSSFEERKTEQEQKREKLKEPGNVEEMW
                     KNGYGSGRNITWLFLALARAAGFEAYPVLVSRRSDYFFNRGTMNPNQLNDNVVLVKLN
                     GSDLYLDPGTAHTPFGLLPWPETYVQGLRLDKDGGSWLRTTLPSSSESQIERKAILKL
                     NQNTGSLEGKLTVTFSGLEALEKRIGERNQDDASRKKFLEDQVKEYIPTGIEVELTNK
                     PDWNSSSPTLVAEYDLKVPGWIAGAGRRALLPIGLFSNTEKHVFEHANRIHPIYFDFP
                     FEKTDDITIELPPGWKVSSLPAGQNEGGKFIGYSSKAEDKNGTLHLTRKIRLGLVFVE
                     QKNYPALRGYFQMVKTGDEQQIILQPGS"
     gene            45379..47421
                     /locus_tag="VGR03_14775"
     CDS             45379..47421
                     /locus_tag="VGR03_14775"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEV1995593.1"
                     /translation="MSSKSYCSQAPEAGRAGARIATLFSALLCVCALGAAPRAAAGDA
                     PAWMHALVNAPLPAHDEKTTAVLLYSEIRITVQSADKIKTVTREAYKILRPNGRSVGA
                     VVAYMNAESKVTSMRGWSIPAQGKDFEVKDKEAVEIAIPNVDGSELVSDARVKVLRIP
                     GSEPGNIVGYEYEEELHPFVLQLNWSFQAAHPVREAHYILQLPPGWEYKAFWLNYPEV
                     KATSTGNNQWQWELHDIKELRSEEDMPPRRGVAGELILSMLPPGGAANRGFLTWNEMG
                     TWYKNLTMGRRDASPEIKQKVASLTSSAPSQLEKMRMLARFSQRDVRYVAIELGIGGL
                     QPHPAGEIFTHRYGDCKDKVTILSSMLHEVGVESYYVVINNRRGSVTPDMPAHPDGFN
                     HAILAVQLPENVSDRSLVAVMNHPKYGRILFFDPTNEVVPFGQLPVYLQANYGLLVTP
                     DGGELTELPSLPAAMNGIQRTAKLTLDGTGTLRGDVQEVRLGAPGVWQRMTLRNVTRD
                     ADKIKPIESLLAHSFATFHITKATVSNLEQSDQPFIYNYSLVAENYGKHAGNLLLVRP
                     RVIGSKSSAMLDTKEPRKYAVEFEGPSRDTDTFEITLPPGYEVDDLPPPVNEEHSFAS
                     YHSKTEVNGNVLRFSRTWEIKQLTVPLEKVEELKRLYHRIAGDERNTAVLKPAGSH"
     gene            complement(47461..47949)
                     /locus_tag="VGR03_14780"
     CDS             complement(47461..47949)
                     /locus_tag="VGR03_14780"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV1995594.1"
                     /translation="MASISSQPNRNAMALAALRIAVGVFFVIFGQYKVFGTQFTLGGG
                     FRTWITQFIDGGAYPFMVPVLRRVVLPLATPLAFLVAYGELAIGLGLVFGILVRPASL
                     AGLLLMSSILFSSAYPGPHAAFWQYFGASLSQSVFAICFAALLIGRSDEIWSLTAWRK
                     RN"
     gene            complement(47963..48598)
                     /locus_tag="VGR03_14785"
     CDS             complement(47963..48598)
                     /locus_tag="VGR03_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995595.1"
                     /translation="MALPEIIAVHFAEEEAGYVTLRPIVRQSFRLADLTDMVVTVTGK
                     DARRVEQIFRAGSVTYNGYRYGWQGFSAEPGEVAALLAPFPENDPARPFRAEEATAAS
                     LEFGGKMQHAPVELKRDEASVRRLLRSRSAWDFLLDAARTASPVYERYSYADRADLFR
                     RTLTPVEGHRLFDGVLAAAPRGLRGRLSALPPPAAIVFHCPRLRQPGSFSS"
     gene            complement(48627..49082)
                     /locus_tag="VGR03_14790"
     CDS             complement(48627..49082)
                     /locus_tag="VGR03_14790"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEV1995596.1"
                     /translation="MSLAERIQKELTGAMKARDELRLSVLRMIKSALKNKEIEKIRPL
                     EEGEALHVLQTLVKQRKESIEQFAKGGRQDLVDKETKELAIVESYLPAGATDAEMDAA
                     VAKAIAETGANSVKQMGAVVKAAKALLEGKTVDGKALSDRVRECLSKLS"
     gene            49224..50816
                     /gene="serA"
                     /locus_tag="VGR03_14795"
     CDS             49224..50816
                     /gene="serA"
                     /locus_tag="VGR03_14795"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEV1995597.1"
                     /translation="MKIVVADKISERGVEILRQPGWNVVLPGTSELEAELVLADALIV
                     RSATRVTPELLEAAPRLRVVGRAGVGVDNIDLDAATRRGVLVMSTPGGNAVSVAEHTF
                     ALLLAMARQVPRLDAAIRAGRWEKSSAAGTELRGKTLGLIGLGRIGSEVARRARAFEM
                     RVLAFDPYISEAAAREVQVELVPLERLLAESDFVSLHAALSPATHNLIHAGTIAQMKP
                     GARLINAARGELVDEAALADALKSGRLAGAALDVFAEEPPKNSPLTGLPNVIATPHVA
                     GATEEAQEEVGTLIAQQVREFLAEGLIRNAVNLPALSAEQYRRVRPYLELAERLGSFV
                     SQAAAVRPARIRIRYAGELAEAGTHLLRSAVLAGILNAVLDEKVNLVNASAVAASRGL
                     TVVEETRRREHGFPNTLEVASLPEPGSATKGFSAEGTVLHDASPRILQIDGISLEGQL
                     GGTILYVRSRDEPGVIGQIGTLLGQQGVNIATFALGRREGRGAEAVSLVRLDGNVSDS
                     AVEPIRRIPAVTEAKLLHLANA"
     gene            complement(50837..52315)
                     /locus_tag="VGR03_14800"
     CDS             complement(50837..52315)
                     /locus_tag="VGR03_14800"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEV1995598.1"
                     /translation="MPGRSAPPLPAMGISKQQLQKLDHVRWLVPRSLREGMLTDALIY
                     ADEKLLEQILSDLSIEQAISVAYLPGIVGRSLAMPDMHQGYGFPIGGVAATNLREGVV
                     SPGGVGFDINCGVRLLASNVERDAAQPKMRELVNQLFRDVPSGTGSQGPVPCSHAELD
                     DVLARGAVWAVEKGYGEAADSEHCEEHGSMPGADPSQVSDRAKQRGRTQLGTLGSGNH
                     FLEVQYVEKIFEPETAEAFGLRENQVVVLIHCGSRGLGHQVCTDFLKTMNEAMRKYGI
                     TLPDRQLACVPSQSPEGQSYLGAMAAAANFAWANRQVITHFARGAFRRLLGEDVRLRV
                     VYDVAHNIAKRERHRVGRSEVDVLVHRKGATRSFPAGSPFIPSAYRAAGQPVLIPGSM
                     GTASYVLVGTERAMEETFGTVCHGAGRAMSRTAAKRGRDARVETQKLEAQGIILRAAT
                     RDGILEEIPEAYKDIDAVIDVVHRAGLARKVARLRPMGVIKG"
     gene            52496..52573
                     /locus_tag="VGR03_14805"
     tRNA            52496..52573
                     /locus_tag="VGR03_14805"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52531..52533,aa:Pro,seq:cgg)
     gene            complement(52699..55830)
                     /locus_tag="VGR03_14810"
     CDS             complement(52699..55830)
                     /locus_tag="VGR03_14810"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEV1995599.1"
                     /translation="MSRSQERIILVCFFLSGVAGLVYEVAWTKALGLVFGHTVYAITA
                     VLAAFMAGLAAGSAYLGKWGGRQARPVTLYGWIEVGIGITGVLSLLGLTAVRALYAAT
                     YHFASGSVPVLETLRFAGSILVLFLPTFLMGGTLPILVAGVGKSSSELRAKLGQLYWI
                     NTAGAVLGALAAGFLFLPWAGLRMTVALAAVCNVLAGSLALALARSPEQPALESTQEG
                     PVRSRDERPMFLLVSFALVGATAMAYEISWTRALATTLGSSTYSFTIMLATFLAGIAL
                     GSRLFEIWVRRNNTVSVATFGETQVFTGIGAVVFLVLFARMPVMAWWMMIATHKSFRG
                     LLLTQFAISGLAMLPAATAFGFNFPLVTSLIASKEERGSTYSRGVGQACAANTVGAIL
                     GCVATGFWLVPRLGAFRTVALAAGVNIALAVFLLMRSRPRRMVELAGSFALAGIVAVA
                     GASRLLYDPAIANFNVAQHPRQYSSRLSADQIAHAADLLYFEDGLNATIAVERDSGSL
                     RLTTNGKTDASTADSVSQLMLGHLGMVFHKAPRKVLVIGFGSGMTASAVARYSEVEQI
                     DCVEIEPAVFHAAPYLQDLNRGVLNDPRLHIILDDARNFLFTTRNRYDVIISEPSNPW
                     IAGVAALFTDEFYEEVRSRLAPGGVFVQWVQAYELFPQDVKMILGTLAPHFSQVSVWR
                     GSLGDLMMLSQGDPGQLSLDRLGQLWSVPALRSDYAMLGLSEPEALIAYHMLDDGDLR
                     RLVGNGARNTDDRTSLEYQAPLAVFGDDTTPVNLRMFRQGRSALLPTSILLGDKRESL
                     LAAARTSLFLQDAERAGLYISELAKASATAETELLRGDWLVAARRPEEARAAYASAAR
                     LDPSSIPARMGLAAAALRVRDYDGSERILREILERQQGYLPAFEIYALVESGRGNWKE
                     ALAWQTKRVLGDPSRPFNQVLFLGDLLIRNGENRDAERLYVELLERDPYRGEARSALS
                     ELYFSEKRWEEARRHLEVLVRYFPAGNPIEYVHLADAYRKMGRIQDAEYCLLRGKKVF
                     PNAIPPFRTDGPN"
     gene            complement(56944..58323)
                     /locus_tag="VGR03_14815"
     CDS             complement(56944..58323)
                     /locus_tag="VGR03_14815"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV1995600.1"
                     /translation="MLNEATITSFRASLRGGLSEPGDPSYDAARKVYNGMIDRRPRLI
                     ARCADVADVMTAVNFGREQKLLVAIRGGGHNAAGLGVCDDGLVIDLSPMNYVRVDPKK
                     RTVLAGGGALWRDVDHATHAFGLAVPAGIISTTGVAGLTLGGGIGYLTRRYGLTIDNL
                     LAVEMVLADGRFVTASGKENTDLFWAVRGGGGNFGVVTSFLFKAQPVHTVYGGPMLWP
                     MEDSAEMMRWYRSFIAKAPDDVYGYFAFLTVPPGPPFPEHLHNKKMCGIVWCYTGPMK
                     KAEKVFKPIRNFKAPALDMVGPIPHPVLQSLFDPLYPPGLQWYWKADFVRTLPDEAIA
                     VHLKHAAKLPSMQSTMHLYPINGAASRVKNGATPWCYRDAMWASVMVGVDPDPANKEK
                     ISTWAKEYWSELHPYSAGGAYINFMMEEGEDRIRATYGKNYDRLAKIKKRYDPANLFR
                     VNQNIKPAK"
     gene            58517..58789
                     /locus_tag="VGR03_14820"
     CDS             58517..58789
                     /locus_tag="VGR03_14820"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEV1995601.1"
                     /translation="MPKFVIEREVPGAGNLSDTQLREISQKSVNILKAMGPEIQWLHS
                     YVTGDKVYCVYLAPNEAAIQEHARRVGLPANRVSAVRRLIDPESAE"
     gene            complement(58860..>59283)
                     /locus_tag="VGR03_14825"
     CDS             complement(58860..>59283)
                     /locus_tag="VGR03_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995602.1"
                     /translation="RELGDRWGIAGTLADLESLAREQRDYPAAHSLYQESMKIFHELD
                     HKRGIARLLECFACSAAAQFEAERSLRLAGAAAALRKNIGAPLTPAEQAKLEAILHLA
                     RQALTNTAGATAWLEGWAMPVEKAIEEVLMPEAATPSC"
BASE COUNT        12178 a        17654 c        17492 g        11959 t
ORIGIN      
        1 ctgggtccat ggatcattcg caacgccacg cttgccgcgt acgccggatt taccccggct
       61 ccggattaca ttctctattt ctattcggcc gccgccgtca aggcaaaggc ggagcataaa
      121 aagttttccc aagtgcagga agagcttggg tacggtgaat ggggctacgt taataatgtg
      181 ctctacctgc agagacatcc agagcaacgc acatggtctc aagcagacat ctttcgattc
      241 gagggtgcgg aggctcggag gaccattttc caagatccgc ggttatacag tcttattcac
      301 ctcaagggat gcactatcgt cattctcgac cccggggtta cagatctgtt gaaaactgtg
      361 cggctctacc ccgaggatgg gggcctatta tctcgagtca tcgatcaagg gcctttccga
      421 gcaacactct ggctcattcg aaactaccca ttcaccatgg tcatgctttt cctgctgggc
      481 acacagttaa tcgtatatta cattctggct ctagcagggc ttcgccgttt gcccatgcat
      541 gcccgcgtct tctttgcctg gacggctttg tattttgtct tagtttccgg agggccggca
      601 gggctggccc ggtatcgtgt gccgatcatg cccttaatat gtatttccgc cggagtggcc
      661 gttgcctatt ggacagcgaa gacggcaccg caaaaagatc cgtctgcgct tcgggatgct
      721 tcggcggagt tctcacgctg agtgcgttca ggacttgcag ctggtacaat ttcggctccc
      781 gcttcgatga caccctagac caaggtcgct gttacggttt ttgaacgatc agaagaaatt
      841 gtcttccgaa ccagcgccaa agccacggca gtttcagata tagagaaacc aaccgcaagg
      901 gatactttgg cgcgtttacc atggtgaatg gcaggaaacg agagattact tcaaggcagc
      961 ggaagcctgc cacctccagc gcctcctgca atgcaagttc ggtcaaggga atgtgatggt
     1021 caaagaaatc ccaataggct ccgggcagat gctttacatt gggtcccatt gcaataagcc
     1081 gccccccgga acgcaaacat ctgtgcgcct cctgtagggt cagcatcaga tgtcctttgg
     1141 tgggcagatg ttcgaaaaag ttgctggtaa acacaagatc caggctcccg gactcaagcg
     1201 gccagcggct tgagcagtcc tgtgcgaaca atttaacttc attctgcaac acattctttg
     1261 acgccgggtt caagtccatc gcgtatcggt gcgcgcactg gatgtgattg atgaattgtc
     1321 catacccaca acccaagtcg agaatgtggt ccgaagggcg aatgtagcgg gagaagaact
     1381 cgcgagtcag aacccgccaa actccattac gatattccag atcagcatca aatcgccggt
     1441 gataaatctt ctccagttgt gcgttaacgt cggccattgt cgtgggtggc ctcttgcacc
     1501 gctgcggagc ttttctccgg tcctgggcgg cgcaaactca ggtccggttg ctttgctgat
     1561 ttcctgcgaa acgcgtaaaa ataccagcgg agataaagtg gcaaccattt ccaggtgcgg
     1621 aagcgtgact ggccgcgagt tcgatcccgc catgtggcgg gtacttctgt gatccggtaa
     1681 ttccccagga aggctttcac ggtcagctca agattgagct cgaatcctcc ccggctctcc
     1741 acggtaaagg acctgaccat gtcgcggtcg tagatcttga acgcgttggt ggcgtcatgt
     1801 gtcggaagac gacgtaacca aaacaaggaa actcccgcca gtcgggacag catctgcttc
     1861 agaaacggac caccgatcaa tcgtccgccc ttcatgtagc ggctgccgac cactacatcg
     1921 taaccctggt tatatagctc cagcatccgg tccacttggg ccaagtcgtc ggatagatcc
     1981 gccataacca ccaggactgc tccgtcagcg gcgacatcga atcccgtcat gatggctgga
     2041 accacaccgc gaccccgccg gttcttcacg gctctcaacc gctgctcgcc gtttgcaacc
     2101 acctgcttga cgacgggtac ggtgtcgtcc tcgtcaaaat cgtaaatgac gtaggcagtg
     2161 aaccctgact tgacgagacg cgcgatttca ccccatagct gccggaagtt tgcgccttcg
     2221 ttgtaaacgg gtatgaggat gcttagcggc ttcatactgc gcgctagatc ttccccactt
     2281 cgatctgctc cgcaatccag ggaatgacct cgtccaaaat ctcgccaagt gacgttgtcg
     2341 cctgaaagcc taagagacga gcggccttat ccgtagccgg tgagcgctcc tgcacatcgt
     2401 attcatacgc tggatcactt acatagcgaa acggcttttt cgaaccatgg atcttccgcc
     2461 atatcaactc ccccagctcc agcaccgtgg tccggacagg ggtggacaaa ttgaagtctt
     2521 cattcatagc tgccggatgc tcaatgcaca accggatgcc ccgtgccaag tccccgccgt
     2581 aggtatagtg gcgcgcctga ttgccggcgc ccaaaatgcg cagcggatcc tgccccttca
     2641 ggaccttttg aaccaggtca ggcacaacat gcgacatggc caacttaacg ttcccactca
     2701 tgatctcccg atcgcagagg gcccgctttt cgccgatgcc cacgcaattg aacgggcgcg
     2761 cgatggtgta cggaagctgg tactgctcgt gcgcgccctg tacgaagaac tcggtggcca
     2821 gcttctggaa tccgtaagtt gaccgcggtg gcgggcagcg gcgttccgcg ccttcgggcg
     2881 tgggaaactc gctggtgctc tcgaatacca tgctggagga aagaaccgtg atcttgcgga
     2941 gcttgcggtt gcggtgcgcc cagacggcgg catcgaagga ggcagccgtg atgcgctcgt
     3001 tctctgcgag caagtcgtag gcaaactcgt gaaagtaggt gatgccgccg atcatcgctg
     3061 cggcggcgac gaagtgatcg cattccccca cgagttcctt gagcaacgcg acattcttgg
     3121 cgtcgccttc gacaaacttg taacgcggat gcgcatcata ggatttctcg gcccgaccat
     3181 acttgctaaa gttgtccagc cctatgacct catgccctgc cccaagcaat tcctcaatga
     3241 gatagccaca gataaatccc gcggccccgg taaccagagt tttcattgtg acttgtgcct
     3301 cgccgtcagg gcggatgccc cggccgtttc ccgctcctgc cagaaacccc atacatccac
     3361 gatgtgcttc ccctggggaa ccggcaaact gaggtacata gaatgtgggg cgccaaggat
     3421 gatgatgtcg gcgcgttgaa tcgcttcatc gagaggcaca aagtccttgt ctttgatata
     3481 gggatccgtg caaagcacct ctcttgcctc cagcatgagc agcttgcgga gcttgtaggc
     3541 aaggctctcc cgtttgtcgt ccgagtctcc cttaaaggcc atgcccagaa tggcgacgac
     3601 tttaccgtgc agcccggatt tgcggagctg ggaaacaatg aaattgggca aaccttcgtt
     3661 gaccagcatg gccgcatggc ccatgaagaa attatttccc gaaaaggcgg acaactgtaa
     3721 tgtgtccttc aaaagacagg gacccgccgc aaagcccgcg cgcgcgaagc ttcccattcg
     3781 ggggtaacgg cgggtcgccg catcatggat gcggtagaag tcgacaccat aactttcagc
     3841 caacagatag aactggttgg aaatggcgaa gtttagataa cgccaactgt tggtaaagag
     3901 tttggctagt tcggcctcga gaggcgttaa ctcgatcaat tcgccgttaa tgatggcaaa
     3961 gaggccccgc gcacgatcga gggcttcggg ttcgaatgcc gaaatgatct gaggaaggct
     4021 gcggatctcc tcgatagcct tgccttcggc aattcgttcc ggacaaaagg caatatggat
     4081 acggcgaccg cggctctgaa cgcggtcata caccagcttg gtcacgcctg gatagacggt
     4141 gctgcgcaga acaagaaggg agttgtcggg gagaagttcc agggtcgaat cgatattgcg
     4201 atacaactcg gtgaccgtag gattcaggtg ctcgtccacc ggcgtaccaa ccacggcgat
     4261 ggccacgtct gcatcccgca ggcatgaatt gtcagcagtc gcgagcagat tcttgctgac
     4321 aaccttccgt agaagctctt ctgccgattt ctccatgaac ggcatgtgac cggaattaac
     4381 ggcggcgatc ttctgatgat cgatgtcgag caaagtcacg ctgatcccac aattggcaaa
     4441 cactattccc agaggcagac caacgtggcc gcatccaccg ataatgacta ttttccctgg
     4501 ggttggcatt ggatttgtag attcactctt ctcttgctgc aagaccactg ttctcccgta
     4561 gcctgacatc gtaaattgtt gatgcctata ttgtaaggca attacgagtt ttgttgaggc
     4621 acagagccgc gggatcgtcc cacgttttgt caactaggac atggcatcca gccggaaaga
     4681 aaaaggcaca ttcggctgat aaggccgaat gtgcctgaat gcaaatacag cttgaacctg
     4741 tcttattgta tctgtataga ccctgctccg gtgggtgcgc ctgcgccggt gttcaggttc
     4801 tctccacctg tgttgatggt cccgttcgcg ccggcaagaa gcccggttgc gctgccgaat
     4861 atgtagttgt gtccaagttg cagcaacgag gctgcgcctg cgccgcccgc ctgcattccg
     4921 atagtattgt ttgcaacagt cgtttccgag actcttcctg tcgctgtgcc agcggcggcc
     4981 tgggctataa caccgatgac actacccgag gcagtgctgt ggaaaacgga catgtcggta
     5041 aaatccccgg caaatatacc gaaggtgccg ttacccagga aattgctgtt ggcaattgtt
     5101 acctgggtgg tggcgcccgt gttgatgctc ttcagtccgc tatcactgtt gttattgctg
     5161 ctggtgccgg tgacgaatac ctgggaagca gcactggttt ggatgctaat gccccgttgg
     5221 gtgaagccga agatgttgca gttctcgatg atcagggcct tgccactgtt gaactggatt
     5281 ccgttgattc cgccgctgat tccgttgatc ctgaggttcc tcaggatcac aacggcgccc
     5341 gctggggccg cgacaatgat gccgctggtg ccagtgacca ggactgaggc cacctggcct
     5401 ccgccgccgt cgagcgtaat ggccttggta atggtgacgg cgccgaatcc gcctggatcc
     5461 agggcgtcga tctctccccc gaccgcggtc ttggaaatgg cgccggcaaa ggtcttgcat
     5521 ggagccgtgc ggctgcaggg gttggcgtca tcgcccacac ccgacaccca cgtccggctg
     5581 gcctgtccat acgccgcggt tgttcccact gcgaaagcga ctaacagcat gactactcta
     5641 tatttactca tattcgttca ctttctcctt ttttcatgat aagcaccggc aaattgccga
     5701 cactcgaagt gccccactct gccctggcca cgagatagca gcggtagatg ctaacacaca
     5761 atatggcctc gggttaatac aattagtagc tatcaccgag atacagaccc tacccaaatc
     5821 cataggcaga gcccgagaga tctcccgatt ggtagtttcc ttggttctga ggaactggag
     5881 tttcatcctt tatcctgagc gcgccatccg gccggctgcc tgcaaacaca agatgcctgt
     5941 gcgacacggg agtcctagtg tctttgacgc acgcaccacc caggagaagt cttccacccc
     6001 atcgcctgtg ccacactacg ggcacaggcg ggggcgttga aaactaattg ccattagcac
     6061 tcacaagatg gctattagtc atgagtgatc tgcagcgtgg actggtggtt cacgcccacg
     6121 ttcgcaggcg cccctgtgaa cgacgatccg aaaatgctcc cttgcacgtc ggcggtgctg
     6181 ttatccgact tttgatgtac gtgagttgtg acgggccccg agtacgttgt ctgcttgaga
     6241 tcgacgatgg acctctgcgc gccattctcc cggaattcca agtgaatcag gcccgcaggc
     6301 ccaggtccgc aagtcgtgat gaaggtggtg aaatcgatcg tgcaggtctc ggtggtgaag
     6361 atggcagggt cgagtgtgct ggtgtcgacg ttcagaacca ggttatgcgt attcacgccg
     6421 gtgaaatctt cgggggggat tggcccgaaa atgttgataa tggttatgct gctgaagtct
     6481 gcgggttctg agacagagga aaactgaagg aaggcagtgg ctccggcagc cgtggtggcg
     6541 ccacgcgaaa cctgcaagct gacgaccgtg aacggagcag tgaactggct gacagaggcg
     6601 aattcgccgt cctgagtgaa cctgatactc tttgcctgtg ccatcaacag cagaggaaaa
     6661 gacaacaatg ccgcaaacgc aaatagccgt ttcatattaa tttctccttg cttcctctcc
     6721 gggaggccct gggacaaaga cttgcgccag gaaaggcaga agacacttat atgccaactg
     6781 taccgaaatt gcaacaaata tatggtgcag tttgattgcc actggaaaat aaaggctgta
     6841 aggtgaggcg ggtagcgccc gctacattag ccaccaggtt taccggaggc cgcaaaagtt
     6901 tcttccctgc gcagataatg gcgctcaaaa tatttctgat attcaccgct gcgcgcttcc
     6961 tgtaaccaag cggtattttc cttataccag cggatggttt ccaccaaacc acgtgcaaaa
     7021 tcccaagcgg gcgcccagcc gagtttacgc ttcaattttg cgcaatccag tgcatagcgg
     7081 cggtcgtggg ccggccggtc ggcaacaaac tggagcaggc tctcggggcg gtcgaggatc
     7141 ttcaaaatcg ctcgagcgac ggtgaggttt tccaattcgc atcccccgcc gatattatag
     7201 acttcgcctt cttttccctt ccgcagaatc agatcgagcg cccggcagtg atctgcgacg
     7261 tggatccagt cgcggacgtt tctgccgtcc ccgtacaccg gcagcggctg gttggccata
     7321 gcctgacaaa tcatgagtgg aatgaacttc tcgggaaact ggaaagggcc gtagttattt
     7381 gagcagcgcg ttatgatgcc gggaaaacca tgcgtcttga ttgcggaaag caccaagaga
     7441 tccgatgcgg ctttactcgc cgcatagggg ctcgtgggcg ccagtgagga agattcatcg
     7501 gatcttcctt ccggaccgag gctcccgtaa acttcgtcgg tgctcacgtg cacgaaccgg
     7561 cccacgtcgc tcctgcggca ggcctccacc atgcgccagg taccttcgac gttgctgcgc
     7621 acgaagggtg aggcatcttc gatgctgcga tccacgtggc tttcggctgc gaagtgcacg
     7681 accgcatcgc aacccttcac gacggatgca acggccgggt cacatacatc catgcgaaca
     7741 aacttcacac gggagtctgc cagaggtact tccagatttc tcaggttccc tgcgtaggtg
     7801 agcttgtcca aaatgaccac ctggtcggat ggatgattgc gcagagtgta atggacaaaa
     7861 ttcgaaccaa tgaagccggc ccctccggta acgagaactt tcacgattgt gacccccctc
     7921 gggacgctag ggtcgtttct gcgtcgccaa cagttttctc tcgcacgagg ctgctggctc
     7981 gccataggga ttcgaaggtg ccggcatccg tccaccatcc atccaaggta tcgtaagtaa
     8041 gctggccgtc agcgagatag gagttattca cgtctgtaat ttcgtactct tgccggccag
     8101 aaggtttgag gctgcggatg cgatcgaaca ccgttgagtc gtagaaataa atgccaatca
     8161 ctgcatacga ggatcccgca attttcggct tctcttcgat tttgacaatt ttaccgttgt
     8221 cgaatacagg cacgccaaaa cgctccgggt caggcacctc tttcaacaca atcttcgccc
     8281 ctcgcccctg cttttcaaag ttctgcctgg ccgcacgcat gctgcgttcg attatgttat
     8341 ctcccaaaat cacacagaca ttgtctttgc ccgcaaaata ttcagccaac cgcaaggcat
     8401 ccgcgatacc gccttctcct tcctggtagg cgaaattgag ctgctgcagt ccgaagtcct
     8461 tgccattccg caaaaggcgc aagaaatcac cggcgctgtt tcctccggtg acaatcaaga
     8521 tgtcctcgat tccggcatcc acgagcgtcc gcaatggata atagatcatc ggcttgtcgt
     8581 aaaccggcag cagatgttta ttcgtcactc gcgtcagcgg gtggaggcgc gacccagtcc
     8641 cgccagccaa tacaacgccc ttcatttggc cctcctttgt ctccgagcgg cggccccgca
     8701 atgctaagga gcaacactct gttggacgct cgtaaccgaa aaatgaaaaa agtcttcacc
     8761 tcctcgaagc aaagacattt ccggaaccaa gcgtgccgtc acctcacgat ttgtggccca
     8821 cgcggagata agactggaac agtcgtagcc gggtcgcaaa ttgtgcgtcc ttcttggcct
     8881 cttccagcgc ctccatggca aataccacca gaattgccaa aagcaacgcg agaattgtga
     8941 cgaccaccac gatcactgcg cggaggggcc aggacttctt ctcaggtgga accgccttgt
     9001 ccagctcctg gatgacggaa gcgtcctttg cctcatcgat tcgcgccgcc tcatattgct
     9061 tggccagaag ctcaaacaat gtctcgcgat acttcacctc tcggagcttg cggatgtatt
     9121 ccaatccggc gccaggtacc ttctccaggg cgatgtccgc aacggagcga ccaccttgcc
     9181 cgcgttcgaa acgccgaagc tgggcgtgca gcgcactcaa ctcctgttcc gcccgtatca
     9241 ggtcggggtt ttccggagtg gcgaaggagc gcatggactg tacctgcact tccttgatcc
     9301 ctacctgggc gcgcaagtcg gcataggcct gcaacatcac tttggattgg ctgtccaact
     9361 ggatgatgcc ggtgctctcc tccgtctgct tgagtgacag ttcagccacg accagctcct
     9421 cgttggcggt cttcacttcc cgctcgaaaa agagccgccg ctttccggca tcagttactg
     9481 ccagcgttct agtgagcttt tccaactcat cgatgtacgc gttggccatt tccgcggccc
     9541 gggtgggaga gcgatcgtca acagaaaccg atatgattcc gtcttttccg gcggagatct
     9601 cggtaaggtt ttccaggcgc cggcgggcat ccatgtgcac catcttatgg tagacgttca
     9661 tcagggagaa acggtcaatc atgcggtcgg ccaccgtgcg gctgcgcaac atggcgacgt
     9721 agagatcact cgcgttgcgc actcccaggc cctggccggc gagacctgcc aggggcccaa
     9781 actgccccaa ctggtttagc atggctgacg aaatggactg gctttgttgt ggaggcagga
     9841 tcttggcgtt ggccgtatag tacaccggca gcaacagaga aaccgctacc gagagcaccg
     9901 cagcgccgac gacaaaatac aggatgaaaa acttgtgctt ggccaggagg atgaacggct
     9961 caagaaagtg gttcttctcc ggcccctcgg acatctggag cagagagact tcttccgata
    10021 cggggagctc gatttctttt tgttcctgtt cggccataaa ttcagtaaac gtggatagtt
    10081 gctagatctt aactcccaat cttctacgcc gctgccaagc ccaggctctg aattcggtaa
    10141 cggcttcctt gcttatggga acaagacttt ggcagcggct gccccgatga caaactgtcc
    10201 gatgatctgg gtccagtcct taaaacctct caaggtcacg cctttatcca gtctttccgg
    10261 cacaacaata gtgtcgccag gcatgagtcg caccatgttg aagctcctca ggagcgcgcc
    10321 attatgaccc tggctgctta cggttgtccc atccgccctg atgataaatg accggccgcg
    10381 gtcaccgttg cgcgttgcgc cgccagccac tctcaggtaa tcaccaaagg tcttgccctg
    10441 cttatagatg aatgcattcg tgttgtaaac ggagccgatc acgttaacag tcgccgggcg
    10501 gaacggtacc agaaggcgat ccccgtcttc caataccaga tccggaaatg cctgcaaatc
    10561 atttgcgtta ggcctgagct ccagtacgat ccgccccgtg gccttcaatt ggcgcagctt
    10621 gtccagcagg cggcgctgac tctcagcagc ctgtcgctcg gcaatgacct cttctccgga
    10681 aagatttctc tgcgaacctg cgttgcgctc gatcgccttt tccagctcat tcacatattg
    10741 gtccagccgc cgttgctggt cttcccgaac agattcgcgc gtaaattctg cgccatacag
    10801 gtatgcctga ggcgtcaatc cgccaacgcg cgtgatcaca tggcggagag attcccccgg
    10861 ctcagcttga taaacgccgg cggagcgaaa ctcaccttcc aggcggacaa acttgctttg
    10921 ctggccggtg ggcacctgca tgtcggactg cgagaagatc gtgatcacat cccccggctg
    10981 cagggtcagg ttttgagcct catccccgac gattgccttg cctaaattga acggcagcag
    11041 atgagttgta agatcctccg ggttcatgcg ctggatcact gcgtattccc agttgatctc
    11101 cgctccggaa cgttttacct cgttcttaag ttcttcctgc ttgatccgct gagcttccgt
    11161 cctgccgacc ctttcggcca agttgtcgcc gcgggtagag ctgcgccggt cgctttgcat
    11221 gtcgcgatcg cgattctgcg aggagttcgg cggctgctcg gtctgctggt tttggacgcc
    11281 tggctggttc gccggagagg tgtccttgtc acctggctgg aactcgcggt cgcgctcccg
    11341 ctccagttct tctctcagct tgaagcttgc accgtcaggg tcgatggcca gcttgttctg
    11401 ccgcttccag aattcttcgg tgatcaggaa gtcacggctc ggaatcaggt ctttcacgtg
    11461 catgctttga tgccagggat aacgccccgg aacagccacg tttccccgca ggatcacagc
    11521 gttttcaaac ttggaggaga ggcgcaagaa gcggaccaca tctccatcat gtaattcgcg
    11581 cgcaagccct tccttattga gcggaaattc gtccgcctgg cggatatgat gatcgccgat
    11641 ccgctccacg accgcatgct ctccagccgc cgtgttggtg aggcccccag cgtatccaat
    11701 cacctcttct agcgcggtgc cttcctttct taattcgaaa accgccggaa tgtttacgct
    11761 acctgctaaa gccaccagcg gccccaccga gggcacgtag atcacatcgc cgggcatgag
    11821 cttggcatcc ttggtcttgt cgccgttcac gatcagatca tacagatcaa aagtcgtgac
    11881 gatctttccg gctcttttta attgaatacg acgcatggag ccgcgcttgg aagggccgcc
    11941 ggaagcaaaa agcgcgtcca caagcgagct taaggagctg atggtgtaaa gcccgggcct
    12001 tctcacttgg ccgacgacaa aaacctggat ggaacgcaac cggcccatgc ttacgctgag
    12061 ctgaaaattc ttaaaaatac gcccaatatg agcctgaaga taatcgtgca gatcgtcata
    12121 cttgactccc gcaatgctaa gagtccctat tttaggtata taaattgccc cggcccggtc
    12181 cacgacggct cgccaatcca cgtcaatctg gccccacgct cgaatcaaca cctcatcgcc
    12241 cggcccaatg atgtaatccg ccggaacctc gacgcggtcc aatggcgcaa aggtcgaagg
    12301 cacgttttgg aagaggttct gcccaaatac ggggagcttg attcccaatg aacttgcaac
    12361 aagttcctga aactcgaggt ctggttcggg cgggagcggc ggcaaacgcc ggcccatccc
    12421 aatcgaatct ttctgttgat cccccccctc ctgggacgga gagatattgg tgatctgcgt
    12481 cggattcact cgagtctttt gtcctgttcc cgacgactgg ccaacaggtt cttgcgtctg
    12541 ctggggttgc tcggaggaag attgcggagt cggaggaatc gccgcctgca ccggcgagga
    12601 agcggcggcg aagaagatgc aacaagtgaa aacaaggacg gttttcagca agatgatttc
    12661 ttcctgtgat cgagtggttg gacggaatcc aagacggcga ggtctccctg gcgtcccaaa
    12721 ccattctcaa acgtcaagtt gcttctccga gaaagactat tctcccagac gatatcagaa
    12781 tggcgcggtg aaaagcgagc agcacattct agggccgctc tggaattggg aagcttcatc
    12841 ctaatccccc tggcgcagtg agcgcgtgta agcccggagc gccgtagcta tccatccatg
    12901 gcgattctgg ggaagcgact acttgacgat aacatctact ttgcagtata aagctcagca
    12961 cagacggcgt caacaggcaa ctgtcgaatc cgtttcgatt gacaatcgtc gccatcgccg
    13021 ggtcgttgat ggtgttgccg gctacgctcg tgcgcggatg gccgcggggg ttgacaacaa
    13081 cggcaggtcg ccgcgagacg gacgaccacc gttccgctga acgccgaatt gcgagggaaa
    13141 agatacaaag tcggcggatt ctccacaatg cgtaagagct tctgcttgaa tgagttaacg
    13201 ggtcttccgg cgcgtgtatg caacttgtct acgcgctctt gtggctccca tccgaactcg
    13261 gtactttata tcggtgcggt ttctcgcatg gggttcccag tgtccttttc catccgaacc
    13321 ttgactctag cagcccttct tgccgcaaat gtcgccgtgg caaatgagaa gattccagct
    13381 ccgtccaggc ctcagtcggc aagctccgca agcgccgaag gcagctcgga cggctgttcg
    13441 atcccgagtc ttccggaaac gaaagcgctc cgggaattct tgcgatctcc atggatggag
    13501 actgcattcc cttcgtcgag tggtcaaaat actccgcagg catcaaagga aacaccggat
    13561 gtgacgatca agagcgtggt cctgaacctg cccaaagatc aactcgcaat ctggaccagc
    13621 cccttccacg cgcgacgccg tgatctcacc tggctgcttc ctttggcagt gacttccggt
    13681 ctgttgattg gaagcgacag ccacagcatg gcccgggaac aatcaaacgc cgtagccgtg
    13741 catcgcagca cacaaatttc gaactacggc gtggccgccc tgctgagcgt gccggcactc
    13801 acgtaccttg ccggccaatg gaatggctcc tctcgcgcgc gggagacggg cctgctggct
    13861 ggtgaagccg cggtcaacag tttcattgtc aatgaggtag tgaagctcac ggtgggaagg
    13921 gagcgaccca ccttgactga cggccggggg cgatttttca cccagttcgg caacagctct
    13981 tctttcccct ccgagcatgc catgctggct tggtcggtag cctcggtcgt ggcgcatgag
    14041 tatcccgggc cgttgagtca gctggtcgta tatgccgcgg ccacagcagt gagcctctcg
    14101 cgcgtcactg cccggcagca tttcccttcg gatgtcgtgg tcggcgccgc gatgggctgg
    14161 ctgataggaa gacagatgta tcgcgcacat tcgacgaatc gcgatgatga ggattacggg
    14221 gcatcggagc acgaccccga atcctccggc gggcctcccg gttccatttt tgtgcccctc
    14281 gatagctgga tctatcccgc cctgaagcgg ctcgcctcgc tcggatatat tcccactcag
    14341 ttcgctgcga tgcagccctg gaccaagcgg gagtgtgtgc ggcagctggg cgaagcggag
    14401 gatctcgcgc aggatttgcc gccggaagcg gacgtccggc agctcatcaa ttcattgaaa
    14461 aaggaattgg gcgcgggcag tgattatgat cattcagcgc agatcgaatc tgtctataca
    14521 cggtacatga atatttccgg ggtgcctctt cgggacacct atcacttcgg ccagacgatt
    14581 tccaatgatt tcggaaggcc ctacgatcaa ggcaacaatt tcattgccgg cgccacggcc
    14641 agcgccgtgg ccggacgatt ctttttttat gtccaaggag agtatgagca tgcgcctgga
    14701 cgcccggcac tgagcgccac gcaacagaac ctggtaaaca gcttggacgg caatgttggg
    14761 ccgctgccta atccgatagg accgcagcca gccccggtcg ctcagattga ccgcttttat
    14821 ccgctggata tgtatgccgg cgtgcaattg ggcggatacg ctgttacttt cgggaagcaa
    14881 agcctctggc tagggcctgg tgaaagcccg ccactgatgc tcagcgataa cgccgacccc
    14941 atgtacatgc ttcgcctcgc gcataccagc cctttttact tgccgtggat cttccggtat
    15001 ctgggcccgg tcgaggacga atatttgttt tcaaaactct cgggccacca gtttccggcg
    15061 cggccctttt tcaacctgca aaaaatcagt tttcacccca ccaggaacct ggagataggg
    15121 ttcacgcgtg cctcattgtg ggccggtgta ggtcatcctt tcactctcca ctcattggaa
    15181 agaaacttct tggcccttgg cgatacgcca gccggtccct ttaatagccc aaatgatatt
    15241 ggcgaccgga agtctggttt tgatttctcc tatcgtcttc ccggtttaag gaattggctg
    15301 acgatctact gcgattctta tagtgacgat gatccttcgc ccatagcaaa tccgagacgc
    15361 gcggcggtga gtcctggagt ctatctttca cgtgtgcctg gcatccctaa gttagatctg
    15421 cgactggaag cggtctcaac ccagtcatta acggctactg acaggagtgg ccagtttctc
    15481 tactggaatc tccgctatca cgattccaat acgaacaaag gatttttgtt cggcaactcc
    15541 actggcagag acggccgtgc acttcagggt tggagcacgt atcatttcgc tcccggcaag
    15601 acgctcgaat tcagttatcg gcaaatcaaa gtcggaaatg gtttccttcc cggcggaggg
    15661 acgcagagcg acgcggtcac gaagttcgcc tggcgcctca atccggagtg gactgttgat
    15721 tccttcctgc agtacgaacg ctggctgatt ccttccttgc ttcccaccga acaacggaac
    15781 ttttcaacga gcatccaggt gacctttcac ccccattggc aaatccacca gcgctagaga
    15841 aggccgggcg ttacttgacg cagcggataa agggcaattg cgtggtggtt tcactgcctg
    15901 atgggttggc aatcgatacg gtccttgctc gacaggaatg cgttgaaaaa ccactcctga
    15961 aataccaaca cgttccaaag tgagtattgc cagtttccgg cgccggagag atgctctcgc
    16021 catttttcgc ggacgatgtc aggatttagc aaaccatgct gcgtaaggct cgttgaggac
    16081 agcaggtcct cagcccagtt gcgcaggggt cccctcagcc agctgtcaat gggcactccg
    16141 aaacccatct tggggcgctc tacccgctct gccgggacat acttatagag cacctctcgg
    16201 aggatccatt taccggttcc atttcgaatc ttgaagtgca gaggcagctt ccaggcgaat
    16261 tcaaccacgc gatggtccag gaggggaaca cgcgcttcca ggctcaccgc catgcttgcc
    16321 ctgtccactt tggtgaggat gtcgcccggc aaatagtcga gtaagtcggt cagcatcatc
    16381 acttcttcca tgcttggaag ccaggcggaa gatgccacgg aggcaacaat acgattcgcc
    16441 acatgagaat tgagcactat ctttgaggga tattgccagt gggaaagcat ccgggaataa
    16501 agctcttccg ggctcgttgc tgataacaga cctgcgaact tatgggcctt gtctccctga
    16561 gccgacatcc gcatactccg cggcaaaagt ggccgaagta aacgcaaggc gtgatccaat
    16621 gtggccggcg ggaccgcccg aatcaatccg gcaatcgccg ttcgaaaagg cttcgggagt
    16681 ctccctacgg aattccaaat ccgcttgttt aagagatagc ggttgtatcc gccgaagagt
    16741 tcatccccgc catccccaga gaggctcacc gtcacagcgc ctcgggctag tttggaaatc
    16801 agataggtag ggatctgcga cgagtccgcg aacggttcgt catacatggc cgggagcagc
    16861 ggaacgacat ccagcgcatc tcggggcgtc aaataaagtt ctgtgtggtc cgtacccagg
    16921 tgctcagcaa ctctcctggc gtcccgcgct tcgttgtatc cctcttcgtg gaacccaatg
    16981 gtgaaggtct ttaccggacg gttgctctgt gcctgcatca gtgcgacaac ggtgctggag
    17041 tcgatccctc cggaaagaaa tgcgcctagt ggaacgtccg caatcatgcg caggcggact
    17101 gcctccagga gtttctggtg aaggagttcg accgcctccg catcagagcc ttcgaacggc
    17161 gattgcaagc tctcccgggc gacatcgctg gccgaccaga acggctgtag gacgggagcc
    17221 tcgccggctt cgctgagagt aaggacgcat cctggctgga gtttctgcag gcctacatag
    17281 atgctatggg gcgccggaac atagctgtgg cgcatgtaga gcgcaagaga gttacgatca
    17341 attgtagggc taaactctgg atactgacga atggctttta gctccgatgc aaaaacaaaa
    17401 tcgccgccaa tcccgccgta ataaagcggc ttgatgccca ggcggtcgcg aacgaggtac
    17461 aaccttcttt cctggcgatc ccataacgca aacgcaaaca tgcccacaaa acggcgcact
    17521 gcgcaatcca ctccccagcg ttcgaaagcc tccaacatca cttccgtatc ggaatggcct
    17581 ctccagagtc gggttccgag ctctgcgcgg atttcctgga agttatagac ctcaccgttg
    17641 aagacgatga cgtagcggcc agagcaggaa aacatgggct ggtgcccctc cggcgagagg
    17701 tcaaggatgg agagccgccg gaatgcgagg ccgattcctg tcgaaccgtc atgaaaggtt
    17761 cccgaatcgt ccggaccgcg gtgcaccaga gccgctccca tcctattcag cgtttccaag
    17821 ggatgttccg gtccgcgttt gctctgccag aatcctgcaa ttccgcacat gaatgctcag
    17881 actccgaatt cgcgtcgcca ggctgctggc ttggcttcga aagaactgcg cctaattggc
    17941 gaaagaacca tcatggtgat gaactatggc gattcggtgc aggatttatc gcaaagacag
    18001 aatgcatgcc aattcctggc ccgtgaaggt ccccctcatg gtcgcgcttc caaccatgtc
    18061 ggcgcgcggg cttcacggaa caccaccaaa gcagcggaat ctgcacagtg tttggaatgt
    18121 acattccgct ctctctggca ggttgaagta taggtgagtc gagcgtttcc agggtaattt
    18181 ttcaaacttg atccgttcgg acggagcaaa tcgggcagat cccccccggg gctcgggcta
    18241 aggttggggc cgtcctgaat gatttgtctt aattcaagga tattgagatt cagattctgc
    18301 ggaagcgtct tgttcgcgcg tggccttcca actttttgcc gataaaccga gcccactgtc
    18361 cgttgcgtaa gatagtcgtt tggcttgacg aactcacagg tgcatcaagt cagactgtcg
    18421 ctagtccgcg tgcttcccaa aaggaaggaa ttcttcctgc ttcaagacag gcaggtgatg
    18481 cctaataatc agatcgttgt cgccgtcctg gcggctgaat actcggaaag ccgtaatgga
    18541 tatgatccgc aagtcgctgg tgaagccggc gctctgcaaa tactgaaggt ccatttgcgc
    18601 tttcaagggc atgatggccc tcatgtaaac ttcttcgacc tcttcgaagg acaatgctgc
    18661 cagcaattct tcttcatttc gaaagataag ggtcgtcggg ctcgtaattc cgggacgcac
    18721 agataagaca tatgctgcct ccttttgaat ggagggttgt tgccagtgcc ctgtaggcag
    18781 gggccgcggc cccacgaaac tcatctggcc gcagaggacg ttaaaaagct ggggaagttc
    18841 gtccagtttc catctccgca gaaatcgtcc ggaccgtgtc accctggggt ccgcagcacg
    18901 cgtcataggc ggaccagtat cgtgattccc attgatcatg gtacggaatt tcaggatcgt
    18961 gaactctcga ccatttttcc cggggcggcg ctgacggaac aggataggcc cttgtgaagt
    19021 cagtttaacg gcaagcgcga cggccaacat cagcggcagg aggaggatta gcagcagaat
    19081 cgctcccaga atgtcaaaca cccgtttgcc ggcggattca caccagggac ttacaaggta
    19141 caaagaactc gattgaagct ggaacggctc ccttgactct ccatttggtt tcattatccc
    19201 ctcaggcccc aatgctgaac tggaatactt tagctttcca caataacgcg cccgcaagtt
    19261 tatatcattt gcggaccaat tgcccttgct gttttgcagt gtctctccat agatgtaaga
    19321 cataagagaa acagaaccag aggaccgcta ccccaccgtc cactcacgcc cagttattat
    19381 ggcagtataa gtccatcctg agtaatgtct ttggttgtat ttcagaagag ccgacaccaa
    19441 tttggcctgc ggcttaagtc attgaaaata tgacttctta gctcagcccc aggcgatact
    19501 cggagcaaac cctcgggtgt cgttctcggg gcagacgatc cagcaaatac tgccccccca
    19561 ttctcccttc cttcgctaat tcagatactt ccacagctct ctgaagttct gcctgcaaac
    19621 atgaatcata cctagcccca ccattaagcg gcaacaactt cttggttgat cgcttttcag
    19681 ggagtacccc gaactgctcc actcttgcaa tgaccgcaag agtccgttgc cacggaaggg
    19741 aagtagctga aacgggatga cggacacttt ctgggtaccc gaactcgcct cccgattgcg
    19801 tccggttccg gaaaggagca atctgggaga cgggaatccc atgtccgcag aattcatgcg
    19861 ccgcgccatc gctcttgctc ttgagaatgt tcgctcgctg ggagggggtc cattcgccgc
    19921 cattgtcgtg aaggagggcc gcgtcatcgc cgagggagca aatcgcgtta cggccttgaa
    19981 cgatccaacg gcgcacgccg aggtagtcgc cattcgcgag gcctgccgga ttctgcgcga
    20041 ttttaacttg acaggctgcg atctttacgc gacctgcgag ccgtgtccga tgtgtctggg
    20101 tgcgatttat tgggcgcggc ccgcgcgtgt tttttatgcc gggacggcgg cagacgcgac
    20161 ggcggcgggt ttcgacgacg ccctaatcta ccaggagttg aatcgagcgc ccgccgcgcg
    20221 gcgcatcccg atgtcgcaac ttctccgcga ggagtctctc gcgatttttt ccgcgtggaa
    20281 agacaaggcc gataaaatcg cgtactgatc aagcctccgg ccacgcgccc agcgcgcggc
    20341 gcaggtccac gagctgtccg agatggtaag aattgtggtc cgcggcgagg agcgattcgc
    20401 ccaagagagt ctgaccattt ccatgcggaa tttttgcgaa gagatccgtg cgcgggctct
    20461 ccaccagccg aaccagccct tgcagatctt tgcgaaatgt ccggacgctc ttttcccacg
    20521 cggcgttgtc cggcggcagg ggcgtcttcg gccagtagcc tccgggaaaa tctggcgaga
    20581 cgtgctttgc gttccggctg aattccagga tgtcccactg cgcgatacgc gcgtgttcaa
    20641 ggagctgcca tgccgtgtgc ggcaggcctg ggacaaggac acctctcttg ctctcgggga
    20701 agtctttcaa ggcgtcctcg aaatggacgt gcgcgccgcc gcccttcagc aggtgaacaa
    20761 gttgtttgcg cagcgccaca tcgtcccgcc gcgtttgctt ccgaatcacc gttcgtcccg
    20821 ccttcgctgc gccttttttc atgacgcctt ttccgaaacc atcacccgtt gcggcattcc
    20881 cggtacggaa tgaagcgtcg agttccgaaa gccggtgttc tcgaacatgc attccaactc
    20941 cgacgccgtg taggcatcgc cggcatccgt ctcagccagc atgatcaggc tgaacgcggc
    21001 tggtgtagga ggcgacacgc gatcttcatt cggaatgaat tccagcgtga cggcacgccc
    21061 tcccggccgc agcgcggcgt gcacccgccg cagcactttc tcgcaggttt ccggatcgaa
    21121 gtggtgcagg aagttcgtca gcagcacaat gtcatagcct tcgcccattt ccgtttcaaa
    21181 cgcgcttccg gggcgcagtg tgtagcgatc ggcgagcccc gcggcgcgcg cattctcgcg
    21241 cgccacttcg agcaccggtt cccaatccag cgcgacgatc tgcgcgcgcg gattcgccct
    21301 cgccagcgtg attccaaaca tgccgtgtcc ggccgcgata tccagcacct tccacggact
    21361 tcccgttctt gcctcaagca actccgcgag gaatgccgcc gccggcacca tgagcggcgc
    21421 catcgagcgc gcgaaggcaa cccagagttc cgcctggggt tccgtattgt caccgccttg
    21481 cgtggcgcag ccgcccttac ggacagcttc cgtgagtttc tggaagcgac ccgtcagttc
    21541 ttccctcccg aggaaaccga tcagcgaagt catgctggct ggcgaacgcc ggtcaagaaa
    21601 cagcgcgctt tccgccgtca aggcgtagcg gttctgctcc tttatcagga agccgagaat
    21661 cgccaggtaa tcgcacaaga tgcggacgcc gcgctcggcc gcgccgatcc ttgcggcgat
    21721 cgcggcaggc tggttcgcgc cttctccgat caccgtaaaa atattcagct caatggccgt
    21781 tttcatcgcc gcagtctgct gataggcgtt gagggtgttg aagatccgct ccggcgtggg
    21841 ttgaggtgcc gctgggcttg ccgtcatgtt tcactgctcc ttttctcagg attgttaggc
    21901 tcgaccagta taatctctcc ccgcactcgc aagccgggga atctgccact cgctggacaa
    21961 ttggggcccc gacggcggcc acgactggcc ctattggaaa catcaaatgc gggaatacat
    22021 ctcttacctt ttctgacagc cgccgtggaa tttacacgct tcgtcagtgg ggacgaaggc
    22081 gtttgatccg attgacacag atgagctctg tctgaatgaa gagcggaaag acggcttccg
    22141 ccgcgggacg atcgtcgagc cggccgcgcg cgctcgcggc tcagctcggc tgagcgccca
    22201 tcgcccgccg ctgctggaag cattcccggc agaaaaccgg gcgtccctgc gtcggacgga
    22261 acggcaccgt cgtctcctta ccgcactgcg agcaattcgt cttggtttcg gtgcgggccg
    22321 gagcggggcc tccggagtgt tccgcgcgtt tcgctttgca cgtcttgcaa cgcttgggtt
    22381 cgttcttgaa gcctttgtcg gcgaagaaca tctgctcgcc ggaggtgaag acgaattcag
    22441 cgtcgcaatc cgagcacttc aacactctat cgtgatattc catgccctgc tccctctctc
    22501 cggtcccggc agggaacaga cacacttcgc acacgattcc acactgagag ggagagccga
    22561 tagaccggga gtgccgggcg cggatccctt ggccatacgg gcgcgaaggt gctacagaaa
    22621 tccaacgatc tctgcctgat ggggcctaaa tcgcgaaggc gcagcgccga atcgcagccg
    22681 aacaggaggt cagtcctgtt cgcgccgctg cttcttgcgt agtcgcttcc gcgaaagcgc
    22741 ttccttagcc cggcgtttct ccccgggttt catatagaag ctgtgcttct tgatttcttt
    22801 gatgatgtct tcttgttgca ccttgcgctt gaatcggcgc agtgcgttct ccagggattc
    22861 cgtctcctgg atgattactt cggccactct ggttcacccc caatccgttc cggattggtt
    22921 tctttcggac ttgcgatagc ggacctggtc acgaccaccg catcccgcca ttatcgcatg
    22981 gcgtagctgg catcttctaa tcaatcggac catgcgaagt caagaagtct ttgtgcaggg
    23041 atgggcgagt ggcggcttcg ccactccttc cctataaatt actctatagt taacttacct
    23101 ccagggccgg ccgttgccgg atcctttccg gcgcggtttt ctccccctgc ccatcgggaa
    23161 gttcccgcgg cacgctgctg ctggtaaaat gaaccgcttc tcgggccgcg cctttttgag
    23221 ggtcagtcat gaccgacatg cgcgatgcaa tcggcgttct cctggccggc ggccagggcg
    23281 agcggctctg gccgctgacg cgcgaccgcg ccaagcccgc ggtgccgttc ggcggcgttt
    23341 accgcatcat agacgtcact ctttccaatt gcctcaacag cgatctccgg cgcgtctttg
    23401 tcctcacgca gtacaaagcc ctcagcctca atcgccacat ccgccgcgga tggtccgctc
    23461 tcgctggcct cggcgactac ttcgagatcc tcaccccgca aatgcgcgtc tcccagcact
    23521 ggtatcaggg cacggccgac gccgtctacc agaacatcta ttcgatcggc agcgagcggt
    23581 ccaagttcgt cttcatcctc tccggcgacc acatctacaa gatgaactac cgcaagatgc
    23641 tcgatcaaca catcgaatcc ggcgccgacg tcaccgttgc caccgttgag acgtctctcg
    23701 aagaggccgc cggccgcttc ggcgtgatgg aaaccgacga gcactggcgc atcaccggct
    23761 tcgaggaaaa gcccgacctg ccaagactcg cgcgccaaaa tgcagataaa tgcaacggct
    23821 ccatgggcgt ctacatcttc aatacgcagc ttctgatccc catcctgctc gccgacgccg
    23881 aggatgcgaa gtccagccac gatttcggca aggacattct cccccgcatc atccacaagc
    23941 accgcgtctt cgcctacaat ttcgaggatg aaaaccgccg cggcggacag tactggcgcg
    24001 acgtcggcac catcgatgcc tattacgagg ccaacatgga cctcgtctcc gtttcgccgg
    24061 tcttcaacct ctatgacaag gactggccgg tgcgcacctg gcagcagcag tatccgcccg
    24121 cgaaattcgt cttcgccgac gccgaccgca tgggtgtggc gctcgattcc atcgtggccg
    24181 gcggttccat tatctccggc agccgcgtgg tccattcggt cctcggctac gacgtgcgcg
    24241 tcaacagcta ctcagagatt tcgcactcca tcatctataa ccatgtcgct gtcggacgcc
    24301 actcacgcat ccgcaacgct atcatcgacc gccacgtcac gttgcccgaa cacaccgaaa
    24361 tcggctacga cgtcgaagcc gaccgccgcc gtttccacgt caccgagtcc ggcatcgtcg
    24421 tcgttgtccg ccaggaatcg ctcatcgagg agcccgaatc ggcgtgacgc tttccgtgtc
    24481 gcgggccgct gtccccgcgc cacacctctg ctacaatccc cgctctgcct cctcaaggag
    24541 ccgctccatg tccactcgca ttgcccgcgt ccacgcccgg cagatcctcg actcgcgcgg
    24601 caaccctacc gtggaagccg acgtcattct cgatggtgga gcgcttggcc gcgcggccgt
    24661 accctccggg gcatccaccg gcgaacacga agcgctcgag ctgcgcgatg gggacaagtc
    24721 ccggtacgcc ggtaaaggta tcctgaaggc cgtcggcaac gtgaacggtg agatcgcgaa
    24781 gggcctcgcc ggatccgacg ccgccgacca gcgcgctctc gaccggaaga tgatcgcgct
    24841 cgacggcacg ccgaccaaat cccgcctcgg cgcgaacgcc atccttgccg tctccatggc
    24901 cgccgcccgc gccgccgccg ccgcgctggg cctgccgctc tacaaatacc tttcgcgcta
    24961 ttccacggac gcttccgcca atcttcttcc tgtccccatg atgaatatcc tcaatggcgg
    25021 cgcccacgcc gacaattccg tggacttcca ggagttcatg gtcatgcccg tcggcgcgcc
    25081 cagcttcagc gaaggcctgc gctggggcgt ggaagttttc cacgcgctca aggccgcgct
    25141 gaaaaagcgc ggctactcga cggctgtcgg cgacgaaggc ggcttcgcgc ccaattgcaa
    25201 atccaacgaa gaggccatcc aagttatcct cgaagccatc acccgggcgg gctacaagcc
    25261 cggcgaacaa gtggccatcg ccctggatcc cgcttcgagc gagttctacg acaaagccgc
    25321 cgccaaatac gtcttcaaga agtcggacaa gtccgcgcac tcgtccgaag agatggccgc
    25381 ccactgggcg cgctgggccg aggagtatcc gatcgtctcg atcgaagacg gcatggcgga
    25441 agacgattgg gcaggctgga agctgcttac acagaagatc ggtgcgaaat cgtcaccgaa
    25501 acgcatccag ctcgtcggcg acgatatttt cgtcacgaat acggatattt ttgcgcgcgg
    25561 catccgtgaa ggcatcgcca acgccatcct catcaagctg aatcagatcg ggacggtcac
    25621 tgaaacgatt gacgccatcg agatggcccg caaggcgggc tacaattcca tcatttcgca
    25681 ccgcagcggt gaaaccgaag ataccttcat cgccgacctc gccgttgcca ccgccgcggg
    25741 ccagatcaag accggctccg cctcccgcac cgatcgcatc gccaagtaca accagctcct
    25801 ccgcatcgaa gagcagctcg ggggctccgc ccgcttcccc ggccgttccg tcttctcttc
    25861 gtagaggagc ggcgcttctt gcgcgagtct cttgcgccta tggcagcgct gcgagcagag
    25921 cggcaggact cgcgttgtct ccaaacgttt ggctgacgtg cacaaagcgg tcaacgttgc
    25981 cggggctgaa gacgaagctc ccgccgagct ggaaggggtt gggtccgagt ttgtgctgat
    26041 gatgaccggc agcgcgcgcc cgtttccggg cggccgcatt gtccccgcgg aggaggtgca
    26101 gcagattcgc ggaacgcaat tccgccaccc ggtaagcttt gcgctgctcg tcgatcagga
    26161 gaggaaaggt gatgcccgca tcctcgcgaa acgcgcgggc ataatgccgg tcacccagcc
    26221 cgatggccgc cagagtcgct cccttctcct ggaaggcttg ctcgtgttcg cgcaactgcg
    26281 cgacgtgttc ccggcaaaag attcagccgt agtggcgaag gaagaccagc acggcgggct
    26341 ggctggccca caatgagccg aggcgaacct cccggctatc cgcgtccgga aggacgatat
    26401 ctgcgagctt gctgctgagt gtctgctcca cgattccttt ttttcgtagc cggcaagcag
    26461 gctaccatac tcccggacgg aggagcgctt ggaggaccac gccgcagatt ggcgggcaga
    26521 ggcacgtctt gcgccgatca tccgctcgag cgcattctct tctttgccgg agcgacctcc
    26581 cggtagctcc cctccacaag cgcgctgact tctttccaat cgagcggcgc cgcgtagacg
    26641 cgcagcgtta cccagccgtg cttgccgatg tacggcgtcc ggaaaaaccg cgcgtccttc
    26701 agaaaaatac cctgcatcag cttgccgacc tgcacgcaga tccccagctc gcccttgtat
    26761 tcctcgagca cggcgaatgt ctttttctcg atttgaaacg tcggatgtcc gaacgtcacc
    26821 gtctctgtcg cacccggcaa ctgcaggcaa gcccgccgca gcttcttcaa gatcgtttct
    26881 tcactcggcg cacgcatcgt ctctacgctc ccagcctgaa ataatcgttg ctgcgcggcc
    26941 tctctttcgc gcgggcgggc cggcgcggcg ctcacgcttc cgcggctgca tatctgcgat
    27001 gagaaaaaag gaaggccgcc agcgaaagct ggcggccttg aaattgttat ggtgaacgac
    27061 ttagaagctg accttgccgc cgaagaagat tcgccgcgtc ggcggtgctc cgttatttac
    27121 gctcggcgtc tgcgtcggat ccccgaaacc gttgaaggcg ccagtgagcc cagcatcgtc
    27181 gaggaatgga tcaacactcg cgtagtttga gtggttgaat acattctgga aggtagcgtg
    27241 gaattggaac gtgacgcgtt cgctgagttt cacgctcttg taaacggaga agttgccgat
    27301 gttcgtgggg gcatcctgca ccgtgttgcg cggggcgttc ccgaacggcg tgccgaagat
    27361 agtttgggac tcaaaggcgt ttatgatgaa ccgcactttg tctttggtgg tggctaccaa
    27421 cgcccccgtt ggcccctgga gggtagtcag gctgatgagc tgggtaactc ccgaggggcc
    27481 gaatgcagtc gtgcaggtgg cagcggaaag ttcatcgccg cagaaggcgc ccacgctggt
    27541 tgcgggagca ctcaggttgc ccaggaacgg ccgggccgtg tcaacgccgg cgaagccgag
    27601 gatgtagccg aagtcatagt agtcgccggc tgcggagaaa gcggcatcac caaattgggc
    27661 cgggttatac cgctggccgg aagctatgat gtagttgccg gagaggcccc aaccgcccaa
    27721 gacgtgtccc agcaacccgt gctgttcctt gaagaagggc agttgctcgg agaacaggat
    27781 gctccactga tgcgggtaat ccaggccgga gaacgagtat tcgcccttgc cgttgacctg
    27841 attcgccggg ttctgagcga agaagatggt gttgccgccg ccaaaggtcg agaagatctc
    27901 gctgacgttg tccagcgtct tgctataggt ataggcgctg cgcaccgtca actgcttgaa
    27961 gaggttgttg gcgcggaact ccacctggag accctggtag ttggagaagc ccgagttgcc
    28021 gcgaagacga acgacgccga aattgcagtt gatgcggcca accggatcgt tgccaaaaga
    28081 gccggaattg ccggtgcgtg taaacaatgc cgaggacgca ggacacggag tcagtccagc
    28141 ggggaccagg ctggggaagt ccgccttcag gtcggcgatg aacggattcc cgtccagggt
    28201 ctggaagaga ccgaaagcgt gattgcccac ataccgggcc tcgatcaccg cgttcttcgc
    28261 aatttcgcgc tcgattccca agctccaagt gtgggttctg tccgggccga acgtcggagt
    28321 gatctgggtc tgagagaact cccgcgggtc aaacacgcct ttctggatgc tggacgccag
    28381 cgacgcgcgc acgttcggcc ccgtcggcac gcccggcagg ggatgcgtgc ccgggctggg
    28441 gactgtattc aggaaagaaa agggcgcggc cgtggaattg ttgaggtaaa tgttgtagaa
    28501 gggcggatcg tatagcaacc gatatccgcc gcggatgacg gtcttgccat gcccggtcag
    28561 gaacccgccc cactgcggcg agtaggcaaa gccaatgctc ggcccaaagc tcttcttggg
    28621 cgagggaatg agggggttgg tacgggctga taccggtatg gcggcccctg gacttgtaaa
    28681 ggtgtccccc aagttgttcg ttcccagggg ctcggtgctc gcccagaatg gggtcgcaga
    28741 ggcttcccgg gctgtcgtga tgtcattgaa caagttggcg ggctggccgt agtaagtcca
    28801 ggtcagaccc aggttcaggg tcaggtgctg accgattttc cagtcatctc cggcgaagat
    28861 gaagctgtca tattcgcgga aatcgagcgt gggattgcca ttggcgatgc ggatacgatt
    28921 gggagtgttg ttcaagaaag aggtccaact gccgaaccgg tactggccgt tgaggttggg
    28981 caggaaggtg ttgggggaac gctggtaggt aaagttcacg cccgccttga gcgaatgctt
    29041 gcccagcacg tagttccagt tgtcctgcgc ctgccaggtg ttcacgatgc ggccctgggg
    29101 cgcgttcgtg gccggaccga agcccagaag cgtggaggaa ttgaaggtga catttgccag
    29161 ggcctggtca atctggccgg cattcggcac ggtgttgccg atggagttgc cgccgaactc
    29221 cacattcagg cggccgaacg agatgcgggc ttcattgacc atgtgggcgg tgaggttgtg
    29281 cgtccagccc agcagcatga cttggctgag cgcgggaacg ttggccacgt acccggctgc
    29341 gcccgtgcca aaagcatcgg tgttgaagga gttgccgcga ttgaagatgt aacgcgccgt
    29401 gatggtgtcg ccccggactt gccagtcgtt cctcagaatg aaatcaaagc cgtggaacgg
    29461 ctgggcaacg gtacgggata cgcccgcaaa tgtcgccgcc ggacaggtgc ctacaagagc
    29521 cactggagtg ccgactactt tcggatttcc acccgaaatg gcgaaggggc caaatttaag
    29581 gagggcctgg aggctttggc tggcagggaa gcaagctgcg agggctgcca ggccggcggg
    29641 agtcggagtg aaagcgctgc tggtgagcgt ggtattctgc ccgagaatct cctcgtcaaa
    29701 tcctccaaag atgaaggcct tgttcttgat catagggccg ccgatggtaa atccgccgaa
    29761 ctcatcgttg aggcgattcg gcttcgtctt acagttgggg tcgcagatat ctttattctt
    29821 gtcgaaatta ttcagggagt tgaggatcga gttgttctcg gttccgaaca ggttgccgtg
    29881 ccaggcattg gtaccggact tggtgataat gttcaccact gacccggcat tgcggccgta
    29941 ctcggggccg aagttgttgg tgaccagaac gtactgctgg acgaactcgg cgtcggtcac
    30001 aaacagccct gggccgccca cgctgttgtc gttgttgttc tggccgtcaa tctcctggtc
    30061 gttgttgcgg ccgcgcaggc cgttaacgga gaaacccaag ccaccattgg tgttcgagaa
    30121 ggaattgtca cgcaccgagc ttacgccggg agtaaacagc gccaggttat ccaggccctg
    30181 gttctcctga attccagcga aggtgtttaa ctccacttgg ccgaacgtgt tggtcacctg
    30241 cgccgtcgag gtctcgatca ggggcgcccc agcggtaact tccaggaaat cggtgacttc
    30301 gcctacagta agctttaacg agcccaagtt cgtgtctacg ccggcaccca cagactcatt
    30361 cttggcgacc gtagtcttga aaccctttgc ctggatttct actttgtagg ttccgaccgg
    30421 catcaagttg aaccggaaaa ggccggagct atcactggtt gtcgtataaa ccgctcctgt
    30481 ctcggcatgc gtcgccttga cttgagcgcc ggttacaacc ccgccttgct ggtcaaccac
    30541 ggtccccgaa atcgaacctt tcaggacctg cccccacgac gcgttgcaga acaccagcgc
    30601 cagggtgagc gcgacgaaaa ctgcaatacc cttcagcttc atgctcatct cggatctcct
    30661 tctcggttga agaaaatgac tgtaagctct cttacggcct tgatgtggtc tggctaagtc
    30721 gttcaatctg caccagttcg gctaagccta aattcaaatt acggaaaaag tcccccgaaa
    30781 gcacaacaac actcagatcc cgtcagttct tcagtttaca ctgcaattga atttccatga
    30841 gaaatagact aatagaatca tgaagatagc ctactcggcc gatcaaaaaa ctcttgtttt
    30901 ctggattccc cggcccggaa acatacaata attggaagga tctctaacat ggggcggtct
    30961 tacctccatc ggggccgcca gttgcatcgc caagccccac cgagtgtgag aaactaccgc
    31021 ctcggagaaa gacatgagta gccccgcgag atctgagagg agtgacgaaa gatcgcccgc
    31081 gccactcgag ctgcagtggg tgcgcgcgca gttcccttcg ctgcagctca ttgtcaatgg
    31141 tcagccagcc gcgtttctcg atggcccggc cgggacgcaa gtgccgaagc aggtgatgga
    31201 cgcgatccag gattatctga tgcgctccaa cgccaacacc tgcggcgcat tcgtcaccag
    31261 ccgccgttcc gacgcgctca tcgcgggcgc acgcgcggca atggcggacc tgttgaactg
    31321 cgcggcggaa gaggtcagct tcgggccgaa catgacgacg atcacgttcg ctttcgcccg
    31381 ggccttcggc cggggattgc agcgcggcga cgaaatcgtg gtcacgacgc tggaccacga
    31441 cgccaacgtc gcaccctggc gggcactgga agaaaacggg gccgttatcc gtcaagtgga
    31501 cattcgtgcg gaggattgca cgctcgacct ggacgacctg aagcggaaga tcaactcgaa
    31561 gacgaagctc gttgcggtgg gttacgcttc caatgccgtc ggcacgatca acccggtcgc
    31621 cgaaatcgtt aagttcgccc acgccgcggg cgcgctggcg ttccttgatg ccgtgcacta
    31681 tgcgccgcac ggcctgattg acgttcgctc tctggactgc gattttctcg cttgctcgcc
    31741 gtacaagttt ttcggcccgc acatgggagt cctctacggc aagaaggagc agatgacgcg
    31801 gctgcggccg tacaaggtga ggccggcgcc ggaccagatt cccgaccggt gggaaaccgg
    31861 cacgcaagtc catgaaggac tcgcgggcgt cgccgcggcc gtggactacc ttgcggagct
    31921 gggaaggcgt tgcgatccgg gagcgcggag ccgccggagc gcgctgcaag ctgcctaccg
    31981 ggcaacgcgg gcgcacgaag cgacgctggt cgcccggctg atcgaaggat tgctggccat
    32041 ccccggtctg cggttctacg gcatcacgga cccgaagcgg ttctccgagc gctgctcgac
    32101 ggtggccgtg cgcctggcga accacacgcc gcttgaaatc gcgaagttcc tcggcgagcg
    32161 cggcatcttt acctgggatg gaaactacta cgcgctgaac ctgaccgagc ggctgggcgt
    32221 ggaatcaagc ggcgggcttc tgcgcatcgg gctggtacac tacaacaccg cggaagaagt
    32281 ggagcggctg ctttgtgcgc tgcatgagtt tgtccagcag caccactgat tttgcaagcg
    32341 gaggcaaatc gcgatcagga gcctggggcg agaaaagagg caacgacatg agcgaagaag
    32401 gtgtcgtagc gtcagcgggc gctgaattga gccaggacga gaaagcttat gcgggcttgg
    32461 cacacgcgtt gatgatttcg acgtggtgga tcggtccact gatcatctat ctcataagga
    32521 gagaatcccg tttcgtcagc ttccatgctc tgcaggcgct gttctggcag atcatactta
    32581 ctgttctcca tttcacgggc atgattgtat ttttcgtcgc agtgttcagt actttgttgt
    32641 ccgtgccgcc gggacaaacg ccgcaggcgc cccaatttcc tgctgggttt ttaatcgcat
    32701 ttccgctgct ctggctgacg tatatgagcg gtttcgtgat ctcgatgacg ctgggaatca
    32761 tctactgtct caaggccatg cggggccaat gggcaggcta tccacttatc ggccgttggg
    32821 cgcgccgaat cgtcaaagtc tgagatggcg aagcggccaa gaccaatcgt cttgacggtc
    32881 ctggacgggt ggggctaccg cgccgaaacg aagggtaacg cgattgccct ggcgcgaaag
    32941 ccgacctatg acgcgctgct gaagaaattc cccaacacgc tgattcacac ctccgggccg
    33001 tacgtgggcc tgccagaggg gcagatgggc aacagcgaag tgggccatct gaacatcggc
    33061 gcgggccgca tcgtgcgcat ggacattacg cggattgacg ggctgatctc gaccggtgaa
    33121 attttcaaga acaagctttt cctgcaggcg atggagcgcg ggaggagccg tcggttgcac
    33181 ttgatgggcc tgctcagcga cggcggcgtg cactcccata tccatcatct ctttgccctg
    33241 ctggagatgg ccaaacgcga gaaggtcgag cgggtcttcg tccattgctt tatggacgga
    33301 cgcgatacgc ccccggacag cggccgggat tatctgcggc agctccagca gaaaatgcgc
    33361 gaactgggct gcggcgaaat cgcaacggtt gtgggccgct actacgcgat ggaccgcgac
    33421 aaccgctggg agcgcgtcga gaaggcgtac cgcgccatgg tacacggcga gaccgagacc
    33481 cactcggacg acccgatagc ggcgatccag cgcagttacg agcagggtct tggcgacgaa
    33541 ttcgtcgagc cgatcggcat ccagcgcggg agcgatccgg agccggttgg tctgatccgc
    33601 gatgacgacg ccgtgatctt ctacaacttc cgcgccgacc gcgcgcgcca gatgacccgg
    33661 gcccttctgg acccggcgtt caacggcttc acagacacga agcggccgaa gaaccttttc
    33721 ttcgtcgcca tgacgcagta cgaaaaaacg tggccgtggc tgcaatacat cctcgggccg
    33781 gaaaagctcg aacacatcct ggcgcaggtc tttgtcgcgc tcaactacag gaatttgcgc
    33841 tgcgcggaaa cggaaaagta cgcgcacgtg acctatttct tcaacggcgg cgtggaaaag
    33901 ccgtacgcgg gcgaggaaag aatcctcgtg ccgtcgccca aggtggcgac ctacgatctg
    33961 atgccggaga tgtcggcagc cggaatcacc gacaccgtgg tgaaggcgat cgagtccggc
    34021 tcctttgacg ccatcatcat gaactacgcg aacgcggaca tggtgggcca ctccgggaaa
    34081 ctcgaggcgg cgatcaaggc ggtggaggcg gtggacgcag gcctcggccg catctaccaa
    34141 gcgctgaagc cgcgcggagg cgcgtggatc gtcaccgcgg accacggcaa cgccgaaacg
    34201 atgattgacc cggtaaccgg cgggccgcac acctaccaca cgacaaatcc cgttccgttc
    34261 cttctggtga gtgaggacga aaagctgcgg ttgcgtgccg gcggctcgtt gcgggacatt
    34321 gcgcccacgt tgctcgacgt gctcggcgat ccgcagcccg cggagatgac ggggcaagat
    34381 ttgcgattgc ggtaagatgg gttgcgatgc tggcccacct gaagactcgc aaggaccacg
    34441 acgcgtcctt tgatccgcgc tcgcttgtgg gagccgctta cgtccgcgtg acgcatagtc
    34501 cggtcgcact cctcatcgct gctgggcttg cgattatcct ttatgtcggc gtgccggaac
    34561 tgcggctagt cctgctggct attcctgtcg gcggcgcact cgtgggtttg ttacttcggt
    34621 tgaaacatcg ttgagctaga tgaatcgaac gaagcccgcc gaaaccaaat tggtcatttg
    34681 cgtgtggcac ccctttacgg agtggaggcc gaagccgatc cttgcggaaa cgctgcggcg
    34741 gcgatggccg gagatgcgcg tcgtgcatct gccggactac gagcggttgc cgcaggagct
    34801 ggcggacacg gacattttcg tgggctattc gctgcggccg cggcagctcc aggaggggcg
    34861 caagctgaag tggatccact cgaccgccgc gggcgtggcg caactgatgt accccgagct
    34921 gcgagaatcc gggctcctgg tcaccaaccc gagcggaatc ttctccatcc cgatggctga
    34981 gcacaccctc ggcctcatcc tggccctggc gaggaatttc ccggattcgg tgcgctatca
    35041 agacacctgc cgctgggcgc aacaggaatt atgggacaag ccgcagcatc tcaccgagct
    35101 gaatgggcaa ctgctgctca tcgtggggat gggctcgatt gggagggagc tggcgcggct
    35161 ggtccgtgct ctcgacctgc gggtttgggg cgtgtcgcgg tcgggcaaag gcgacgccgc
    35221 gctcgcggag cgcatgctgc cggcatcgca gcttcacgcc gcgctgccgg aggcggacta
    35281 tgtcgtgatt gctgcgccgg agacgccgga gacgaagcac ctgatcggtg ccgcggagct
    35341 tgcacggatg aagcccacgg cgcggctgat caacgtcgcg cggggatcac tgctggacga
    35401 agctgcgctc gtgcatgcgc tggaaacggg cgcattggca ggcgcagcga tcgacgtcgc
    35461 ggccgccgag ccgctgcctc cggattcgcc cctgtggcgg gcgccgaatc tgattatcac
    35521 gccgcacacg agcgcgctga gcgaccgtct gtgggagcgc gagacggcat tgttggtgga
    35581 gttgctcgag cgctggttcg atgggcggga gatgaccaat cgggtcgacc tggcgcgcgg
    35641 gtactagaat ttctcgcggg cggacggaag agaacagact cagtcgccgg cattgtcctt
    35701 cgcagcgatg tagccctggc gggcgtacac aacgatcttc ggcagctttt ggtttttttc
    35761 cttttgtttt tgatccctaa catcgagcag gctgccgtca ggctccacaa gctccacctt
    35821 gatcttacgg aatttgccgt cgcgcgtgcg gttggtgggc gtataggcca ggctgtattg
    35881 attgcggagg taggctacga catcctggta gatccccgga atctcgccgt cgaactgcgg
    35941 aaaccacgaa tgtccgcccg tcatctctgc aaaagcatgc aactggttct gcgcctggta
    36001 gtaaccgagc cggcctacag ccccgagtcc gccgcgcgca tcaagggcat ccacgtaacc
    36061 ctttcccaca ccgacggcga agatggtcac gtcggtctgt ttgagctgtt tcagtgtctg
    36121 gtcgagcgtg tgttttgaga aagtatcaat tcccgaagcg aggatcagga tggatttttt
    36181 gcctctcacg tctttgagct tgtccaacgt gtccagcagc gcgtcataga tgcaggattc
    36241 gctgaatccc gggaaataga ggcgatagat cgcttggcgc acttcttcct tgttttgggt
    36301 gaagtccact tcgagtttcg gcctcatgtc gaagatgacc agggccaccc agtctttcgg
    36361 gttcaggcgg tggaaaaacg gatcagccca gtacttcgca tacgcggcga aaatgcctcc
    36421 atacaccttg ctgaattcca tcagcatgac cagggtgatc ggagaatcgc tgggagagaa
    36481 gttcgtgatg gtctgcggga cgccgtcttc gtagatgcgg aagttctctt tcttcaggcc
    36541 gcccagaatg tcgccgtgct gcgtcgtggc gacaacgtcc acagtgacaa ccgggatctc
    36601 cacggagatg gcaacttcgc cttgttgctg tggctgttgc gcgggaggct tgggggcttc
    36661 ctgctgcgca ccggccgcca ccggcatctc gcccagcggc aaaaccagcg ccgcaagcag
    36721 aattccgata aggattcccc acgcggcagc tctcatggca gtctccacct cacttccact
    36781 ttatgctgac acaggcagtg caggatgaca atgtcccgcc ggcaatttgc ggcgacgggc
    36841 gtaagagtta cgccacgcgc gatttcggcg gcggtccgcg cctgcacccg catgatagta
    36901 tgaagcgttc ttggtagaaa acgtttccca gtcgattctt gctcggaggg gacaatccgg
    36961 atgcacaggc gcgggtcggc gggcgtagcg atgttctctc tgttcctggc cgtcgccgcg
    37021 gcattccccg tgagcgcaca gcagacgccc gctccgatcg cgccgccggc agccgcgccg
    37081 atcgaggaca caaccgcgct tgctagggaa gccgtccggt ggctgaccga cttgatccgg
    37141 atcaacacga cgaacccgcc cggaaacgaa caagcggcgg caaaatacat cgcaggaata
    37201 ctcgagaagg aaggcgtgac gccggagctg ctggaagtcg cgccgggcag gagcgccgtt
    37261 gtggcgcgac tgcgcagcgc ggcgctggcc gacccgtcgc gcgcgctgct gctcgtggga
    37321 catctggatg ttgtgggtgt cgaacgcgcg aggtggacgg tggatccctt cgccgccgtc
    37381 gtgaaggacg gttatctata cggccgcggg tcgctggacg acaagggaat gctggcggca
    37441 aacctcgcgg tgttcatcgc cttaaagcgg gcgaacgcgc ggctgaaccg cgacgtcatt
    37501 tttctggcga cgtgcgatga agagcgcgaa ggcaacgccc cggacgccgg catcaagacg
    37561 ctgattgcga aatactggga caaattcgcg gcgggatttg cgctgaacga gggcggatac
    37621 gtcgtcgtga aaaacggcaa ggtactttac gccgcggtcc aggcaatcga gaaagtggag
    37681 gcgaatgttt ccgtcgtggc gcgcggcccg tcgggccatg cctcgcaacc gggcaaggac
    37741 aacgcggtcg tgcacctcgc ggcggccatc gcgaagatcg ggacgtattc cgcgccggtg
    37801 cggccgacgg ccatcgtgcg gcgctttttt gaggagctgg ccaagattga agaccctgaa
    37861 atggggaaat ggatgcgtgc gctggaaact tccgaccggg gcgagcatgc gcagcgcgtc
    37921 atttccgaaa ccaacccgct ctggaactct atgctgcgtg attccattgc gccgacaatg
    37981 ctctcggcgg gcgcctttcc gagcgcgata ccctccgagg cgcgggccgt cctgaatata
    38041 cggctcgtgc ctggcgattc ggtgaacgct ctggtggcgg agttgaccaa gctggtgaac
    38101 gatccgcaag tgagattcga agtgatgccg ggagcgggct tcccggcgcc gccttccgcg
    38161 ctggacagcg aactttaccg tgcgatcgaa aaggcaagtc aggagcagtt cccgggaatc
    38221 gccgtattgc cgttgcaggg cacgggaacc accgactcgg cacagttgcg gctgcacagg
    38281 gtggaggcgt acgggttgac gccgttcccg atgacggaag aggatttgag gcgttcgcac
    38341 gcagatgacg aacggattcc gctgtccgca tttgccctgg gcgtgcaatt cctctatcgt
    38401 atcgtttcgg agtttgcgat cgcgaaatag ccggacgcgg cacggaccgc ttgcccgtcc
    38461 cttcgagccg ccgggtcaga accacacatg cttttccgcc gcagcaaaat tgtctgcacc
    38521 atcggaccgg ccagccgcac tccgcgcgtc atagaaagaa tgatgcgtgc gggcctggat
    38581 gtcgcgcggc tgaatttctc gcacgggtcc cacgccgacc acgcgcagag cattgcgctg
    38641 ttgcgcgaga cctccgtaag actccggaag ccgatcgcca ttcttgccga cctgcaggga
    38701 ccgaaaatcc gcaccggcgc gttggccgga ggcaagcccg tgacgctaag ggcgggccag
    38761 cgcttcgtga tcaccacggc gcgcgtgctg ggagactcga cgcgggtcgg gacgacgttt
    38821 acgccgctgc cgcgcgaagt ccacccgggc gaccgcgtgc tccttgcgga tggcctgatc
    38881 gagctgcgcg tgcgccaggt gcgcggccgg gaagtcgcct gcgaagtggt gaacggcggc
    38941 gaactgggcg agcacaaggg cgtcaacctg ccgggcataa agctgagtgt gccggcgctg
    39001 acgaagaaag accgcgccga cctggccttt gcgctggcgc acggtgtgaa ctacattgcg
    39061 gtgagcttcg tgcgccgccg ggaggacgtg caactggcaa aggcggcgat ccgccgcgcc
    39121 ggaaaagaca ctccggtgat cgcgaagctg gaaaaacccg aggccattga aaacctggat
    39181 gccatcctga tggcggccga tggagtaatg gtggctcgcg gcgacctggg cgtggaaatg
    39241 agtccggagc gggtgccggt gatgcagaag gagatcattg cgcgagcgcg ggaattccgc
    39301 cgcccggtca tcacggccac gcaaatgctc gagtccatga cgcagaatcc gcggccgacg
    39361 cgtgccgagg ccagcgacgt ggccaatgcc atctttgacg gctcggatgc cgtcatgctg
    39421 tcggcagaaa ccgcggccgg aaagtatccc gtcgaagcgg tggcgatgat ggcgcgcatc
    39481 atcgaggagg cggaagagag cgtgcatgaa ttcccgcggc cggcgccgag cgatcagctc
    39541 aaagtggcgg aaacggtggc ggaactcgcc tgccacgctt cgcgcgagtt gcacatgaag
    39601 gccatcgccg ttttcaccca cagcggattc acggcacagc tcgtttcgcg ctaccgcccg
    39661 caaacgccca tcatcgcctt ttcgccggaa gcggagacgc ggcgccggct cgcgctgatc
    39721 tggggcgtcc tcccgcgcag catctcaaac gtgcggaaga ttgacgggct cgcggcgatc
    39781 gcggaaaagc gcctgcttga ggaacgcctc gtgcgccgcg gcgatatcat cggcattctc
    39841 gccgggacgc cgatgggcat ccgcggcaca acgaacttca tgaaattcca cgtcatcggg
    39901 ggtggcgcgt gagcgcggcg ccgggcgttt ccgagaagtt ggctgacctc agttgatcca
    39961 gcggtccggg cgcgggggcg gctcgtcctt gtgatccttc atgaaaactt cgaatctctt
    40021 gcgattgcgc tggtatttcc agtcggatag cccgttgcgc aaacggtaga ggaaagagct
    40081 gcggcgcagg tagagccagc cgacgagcat tcccccgagg tgacagacgt ggctgacctt
    40141 gtcgtccccc aggccgaagg tgccgagaaa ggtgaacgcc gccatgaccg ccaccgccgt
    40201 gcgcatgcgt attcgcacgg ggatcgggaa aaacatcact tcccggtccg gaaaaagaat
    40261 cgcgttggcg atcaagattc caaagatggc tccggacgcg ccgatcgtgg gaacgtagga
    40321 gaacccagcg ccgaacagga ccggcacggt cttcacgatc acgttgatca agccggcgcc
    40381 cacgccggta aggaagtaat actgcaggaa gcggttgcgg ccccaaacga gctcgagctc
    40441 cttcccgaac atccacagca gaagcatgtt gaagaagatg tgccagatcg ttccgacctc
    40501 gtggaagaag aggtaggtga atggctgcca gatgcgcagg ccgggaacca cccccgcggg
    40561 cacgagaccg aaccacatca gcaaccggtg gtgcgcggaa gctccccagt agtggttcag
    40621 gatgacctgg aagagaaaga ccgaaaagtt ggcgatgagc aggagccgca cgcccgcggt
    40681 gatgtagaaa ttaaagctca cccgataatt acgaactccg gagcgcgagg ccattacgag
    40741 accagtgtac ggcggcggga cgcacccgtc aaagcgacga actgtcgcgg ctgccgtact
    40801 gctcttccac ggcgcgccgg ccgctgtctc aggctaatgc cgttccaact tcttgcagga
    40861 taatcgagct gaccgtcggg gcggtattcg cttggttcga cgaagtcgca ggcgcatcaa
    40921 gttggaacag cgcttaattc aacttcttct tcgcgaaaaa caagagcgaa ccggcaagga
    40981 acagaaacat gaccatcccg aagaggttgc tctgcgccag ggagccgagg attttcggac
    41041 caaaagcgaa gagatctgcc cgtagcggga gatggaatat cgaaatgacc cagtgcggaa
    41101 ggtgcgcaga aatgtccggg tcctgaagaa ggttgatcac gatggcgagc aggccgaaga
    41161 tgcctccggc ggcgatcagc ccgctggagt agagcgctcc cgggctggcc tcgctctcgg
    41221 aatgctcctt cttcgaaccg cgctcggcga gccagcggac taggccgccc gcaaagattg
    41281 ccgcagtcgt cgcgatggac agataggaac cgacggcgaa ggcgagcgaa cggacgccga
    41341 gcagctcgac ggcaaagacc agagcgactc ccatcaaaac gagcctccaa ggaagacgct
    41401 ggtttaggat gccgctgatg accgtggcca tcaggcgagc ctgcggcgca ggagccttct
    41461 cgctgccgat cccctggatc cactggacct cgatctgttt tgtcgctgga tcgtagaggt
    41521 actcgccatc ggcaatttcg cccgagccaa gggcgttgac gagctcatac gtcttgcctt
    41581 tgtactcgaa gcggtccttt tcgatcttca cgccggcagg aagcgattgg acattgagcg
    41641 gtatctgcgc gggcgtgtat ttctcgaggc ccttgttcat cagcgagagc gtcaagccga
    41701 cggcgatcgt cgagacggtg acgccgacca tgagtcccca ctgctgccag acgggcgtgg
    41761 atccgacgag gtaaccggtc ttcaggtcct gggaggtggc cccggcgatc gccgcggcga
    41821 tgcagacgac gccgccgatg gtcagcgcca ggacggcgta gttcggcgcc gtccagtggg
    41881 agacgagaaa gacagcgcaa gtggccatta acgttgctat ggtcatgccg ctgatgggat
    41941 tggaggaatt gcccagcagg ccgctgatgc gcgaggcgac ggtcacgaaa agaaagccga
    42001 agacgaccac gaatacggcg gcgaatatat tggaaaggag gctcgtttgc gcgcccggga
    42061 taggcttaaa cgtcagcaac agccacatca tcacgatgat ggccagcgag ccgagaacga
    42121 ccgtgcgcat gggcatgtcc cggtcaatgc ggctggtgcc cgtggcaccg gactttcctt
    42181 ccgcgcggag gtctttcatg ccggagcgaa gcgccgaaac gatcgtcggg agggtccgaa
    42241 ggagcgtaat caggcctgcc gcggcaacgg cgccggcgcc catcgggcga atgtaacttc
    42301 gccacatgtc atccggcgac atgagcggta tggggatcgt tgccgggtaa atgggtgtgt
    42361 tcccggaaaa ctggccgaag aatcttatcg caggcatgac gaggagccag gagacgactc
    42421 caccggcaaa cagcagccct cccaccttcg gcccgatgat gtagccgacg ccgaggtact
    42481 ccgaggtgat ggccgcgcga aaagaagcgc cggggaacca cacgggccgg tgttctggct
    42541 ggccggtcca cgcctgcgct gtgttcatca gaaatgtgta gacgcccccc agccccaatc
    42601 cccagaagac gcgcccggca aaggaaccgc cgcgctctcc ggctacgagg acgtcggcgc
    42661 aggccgtgcc ttcggggaag gtgaggctgc catgttcttt gacgatgagc tgccggcgca
    42721 gaggaatcat gaagagaacg ccgagccatc cgccgagaag agcgagcggg aaaatgcgca
    42781 agtaggtgaa gtacgaacca aagcccagaa agatgagcgc gggaagggta aacatgacgc
    42841 cggcggcgag ggattcgccc gccgagcccg tggtctgcac gatgttgttt tccaggatgg
    42901 tcgagcggcc gaaagcacgc aggatactga tggacagaac ggagatgggg atagaggcgg
    42961 aaacggtgag tccggctcgg agaccgacgt acacggtgac cgccccgaac aacaatccga
    43021 acagggctcc gagcaaaaca gcgcggagcg agaattcgat ggggctttct tcggtgggga
    43081 cgtaggtgcg gaattctccc ggcggcgcgg tggaactcca cggtcgtgtg ctcaaagtag
    43141 cctccggacg ggaaaagcta acgcccaaac caaccacgcg ggccaggccg gcgcggctgc
    43201 gcccgggatt atgccgcaga cgcgaacaat cgtggtagcg gtattgtgac gacgcagggc
    43261 tggaaccgtt tgaaaggctc ggctgctgca ttgacaagcc tgcggcggac gggtattttc
    43321 tgctccagct aagtagacaa gtcagcaacg gccgggtgga agccgtggag gagttgtcgc
    43381 cctcctacga tccgatatcg gatggagtcg tcttcacgcg gccgtggcca gccagattca
    43441 tcgggaggca gaagatgaat ggttgtcgct cggctcgtct ctatcttgtc ctcgcgtccg
    43501 tttgctggtt gctgggcgcc ccgcgaacgg gggcgggcga cgattggccg cctgtctccc
    43561 aagaagagct gaaaatgacc agcgagccgc aggcccccgg cgcgcccgcg atctacctct
    43621 ttcgcaaggt agaccgtgac gaccagaacg cccacgaaga caactatgcc cgcatcaaga
    43681 ttctgaccga ggaaggacgg aaatatggaa atgtcgaatt gcccttcatc aaggggcaac
    43741 agagtgtccg cggcatcaag gcaagaacga tccggcccga cggcaccgtc gtcaatttcg
    43801 acgggaaagt ctacgaacag acggtcatca aagccaaggg cctgaagttc ctggcgaaaa
    43861 cctttacgat gcccgaagtg caagtgggga gcatccttga ataccgctat gtctacgatt
    43921 gggaaggata catctttatc gattcacgct ggattctgag cgaggagctt tttaccaagc
    43981 atgcaagttt ttcgctcaag ccgtacctgc gctatgcgct gcgatggagc tggccgacgg
    44041 ggcttcccgc tggcactgta cccccgaagg atgagcacgg gacgatccgc ctcgattcgc
    44101 aaaacattcc ggcatttcaa ttcgaagact acatgccgcc ggagaacgag ttgaagtttc
    44161 gcgtcgattt cgtatacctg ccggcggagg acagcccgga gaaggatccg gataaatttt
    44221 ggaaaagaga gggcaagaaa ctctacgacc gggtggaagc ttttgcggga aaacgcaaag
    44281 cgatggagca ggcggtcacc cagattgtcg agccaaatga tccgccggag gtgaagctgc
    44341 agaaaatcta tgcgcgggtt caacaggtac gcaactcgtc ttttgaggag cggaagaccg
    44401 agcaggaaca gaagcgggaa aagctgaaag agccgggcaa cgtcgaggag atgtggaaaa
    44461 acggctacgg gagtgggcgg aacatcacat ggcttttcct ggcgcttgct cgggctgctg
    44521 ggtttgaagc ctatccggtg ttggtatccc ggcgtagcga ttactttttc aatcgcggga
    44581 cgatgaatcc gaatcagctc aatgacaacg tcgttctggt gaagctgaac ggcagtgatc
    44641 tctacctgga cccggggacg gcacatacgc cgttcggctt gctgccctgg cctgagacct
    44701 acgtgcaggg cctgcggctg gacaaggacg gtggcagctg gctccggacg acattgccct
    44761 caagttctga gtcgcaaatc gaacgcaaag ccatcttaaa gctgaatcaa aacacaggat
    44821 cgcttgaggg aaagctcacc gtgacctttt ccggcctgga ggccctcgag aaaaggattg
    44881 gggaacgaaa ccaggacgat gccagccgga aaaaattcct cgaggaccag gtcaaggaat
    44941 atatcccgac gggaatcgaa gtcgagctga ccaacaagcc ggattggaat agctcgtcgc
    45001 ccactctggt cgccgaatac gatttgaaag tgccgggatg gattgccggt gcaggacgcc
    45061 gcgctctcct ccccatcggg ctgttcagca acacggaaaa gcacgttttc gaacacgcga
    45121 accgaattca tccgatctat tttgacttcc ctttcgaaaa gacggacgac atcacgatcg
    45181 aactgccgcc cggatggaag gtgagcagtc tgcccgccgg gcaaaatgag ggtgggaagt
    45241 ttattggtta ctcaagcaaa gcggaggaca agaacgggac tctgcacctg acgcgaaaaa
    45301 tccggctggg attggtgttc gtggagcaga agaattaccc tgctttgcgc ggctattttc
    45361 agatggtgaa gaccggcgat gagcagcaaa tcatattgca gccaggctcc tgaagccggg
    45421 cgagcaggag cgcggatagc gaccctgttt tccgcgctcc tgtgcgtgtg cgcactgggc
    45481 gcagcaccgc gcgccgcggc gggggatgcg cccgcgtgga tgcacgcgct ggtgaatgcg
    45541 ccgcttccgg cacacgacga gaagacgacc gcggtgctgc tctattccga aatccgcata
    45601 accgtgcaat ccgccgacaa gatcaagaca gtaaccagag aagcgtacaa gattctccgg
    45661 ccgaacgggc ggtcggtagg tgccgtcgtc gcgtatatga atgcagaatc aaaagtcacc
    45721 tcgatgcgcg gctggagcat tcccgcgcag gggaaagatt tcgaggtaaa ggataaggaa
    45781 gcggttgaga ttgcgattcc taatgttgat ggaagcgagt tggtctccga cgcgcgcgtc
    45841 aaagtccttc gaatccctgg atcggaaccg gggaacatcg tcggatacga gtatgaggaa
    45901 gaactccacc cattcgtcct gcagctcaac tggtctttcc aggcggcaca cccggttcgt
    45961 gaggcgcatt acatactgca actgccgccg ggatgggaat acaaggcgtt ctggctgaac
    46021 tacccggaag tgaaagcaac ttccaccggg aataaccagt ggcagtggga actccatgac
    46081 attaaggaat tgagaagcga agaggacatg cctccccgga gaggggtggc cggggaattg
    46141 attctgtcca tgctaccgcc cggcggcgca gccaaccgag gcttcctgac ctggaacgaa
    46201 atgggtacat ggtacaagaa cctgactatg gggaggcgcg acgcctcgcc ggaaatcaag
    46261 cagaaagtgg cgtcgctgac atcgtcggcg ccgtcgcagc ttgagaagat gcgaatgttg
    46321 gcgcgatttt cgcagcggga tgtccgctac gtcgccatag aattggggat cggcgggctc
    46381 cagccgcatc ccgcgggtga gattttcacg caccgttacg gcgattgcaa agataaggtc
    46441 acgatcctaa gttccatgct ccatgaggtc ggcgtcgaat cgtactatgt ggtgatcaac
    46501 aacaggaggg gctctgttac gccggatatg cccgcccacc ccgatggatt caatcacgcc
    46561 atcctcgccg tgcaactgcc cgaaaacgtg tctgatcgct ctctggtggc agtgatgaac
    46621 cacccgaaat acggccgcat tctttttttt gatcccacga atgaggtggt cccgttcggg
    46681 caattgccgg tgtacctgca ggcgaactac ggattgttag tcacgccgga tggcggcgag
    46741 ctgacggaac ttcccagttt gcccgcggct atgaacggca tccagcggac cgccaaactg
    46801 acgctcgatg gcaccggcac tctgaggggg gatgtgcagg aggttcggtt gggggccccc
    46861 ggcgtgtggc agcgcatgac tttgcgaaac gtgacgcgcg acgccgacaa gatcaagccc
    46921 atcgaatcgc tccttgcgca ttccttcgcc acttttcaca tcacgaaggc gacggtctcg
    46981 aatctggagc agtccgacca gccgttcatc tataactact cgcttgtggc cgagaactac
    47041 ggcaagcacg cgggtaactt gctcctcgtc cggccgcgcg tcatcggcag caaatcgagc
    47101 gctatgctcg atacaaagga accgcgcaag tatgccgtcg aattcgaagg cccctcgcgc
    47161 gatacggaca cctttgagat caccctgccg ccggggtacg aggtggacga ccttccgccg
    47221 ccggtcaatg aggaacacag ttttgcgagt tatcacagca agacggaagt gaatggaaac
    47281 gttttgcgtt tttcacgcac ctgggagatc aagcagctca cggtgccact cgaaaaggtc
    47341 gaggagctga agaggctgta ccaccgcatc gccggcgacg aacgcaatac cgcggttctg
    47401 aagcccgccg gctctcatta gcttgaaatc cccccaggcc tcgtctccca tggaacggca
    47461 ctaatttcgt tttctccacg ccgtcagtga ccagatttcg tccgaccgcc cgatcagcag
    47521 cgcggcaaag caaatggcga aaaccgattg gctcagcgac gcgccaaagt actgccagaa
    47581 ggccgcgtgg gggccggggt atgccgaaga gaacaggatc gatgacatga gcagcaggcc
    47641 ggcgaggctc gccgggcgca ccaggatgcc gaagacgagg ccgagcccga tggccagttc
    47701 cccgtacgcc accaggaacg ccagcggcgt ggccagcggc agtacgacac gccgcagtac
    47761 cggcaccata aaaggatagg cgccaccgtc aatgaactgc gtgatccagg tgcggaatcc
    47821 tccacccagc gtgaattgtg tgccgaagac cttgtactgc ccgaaaatca cgaagaatac
    47881 gccgaccgcg atgcgcagcg cggccagggc catcgcgttt cggttcggtt gggaagaaat
    47941 cgaggccatg ggaggtgctc ctctatgaac tgaagctccc cggctgccgc agccggggac
    48001 aatgaaacac aattgccgcc gggggcggaa gcgcgcttaa gcgcccgcgc agtccccgtg
    48061 gcgccgcggc aagcacgcca tcgaatagac ggtgaccctc tacaggcgtc aacgtacggc
    48121 ggaacagatc ggcccggtcg gcgtaggaat atctttcgta aacaggggac gcggtgcgcg
    48181 ccgcgtcgag cagaaaatcc cacgcgctgc gagatcgaag cagccgccgc acgctggctt
    48241 catcccgctt caattccacg ggtgcgtgct gcattttgcc gccgaattcc aggctggccg
    48301 cggtcgcctc ttccgctcgg aagggacgcg cgggatcatt ctccggaaac ggagccagca
    48361 gcgcggcgac ctccccgggt tcggcggaaa acccctgcca cccgtagcga tacccgttgt
    48421 aggtcaccga gccggcccgg aaaatctgtt ccacgcgccg ggcgtccttc ccggtcaccg
    48481 tgacgaccat gtcggtcaga tcggcgaggc ggaaggactg ccgcacaatc gggcgcagcg
    48541 tgacgtaccc ggcctcttcc tcggcgaagt gaacggcgat gatctcgggt agggccacgg
    48601 catctgtgcc ggcaaagaaa tcgttgtcaa ctcaacttgg acaggcactc gcgaacccgg
    48661 tcgctgagcg ccttcccgtc caccgtcttg ccctcgagaa gggccttcgc cgctttgacg
    48721 acggcgccca tttgcttcac ggagttcgcg ccggtctcgg cgatggcttt agcgacggcg
    48781 gcatccatct cagcgtctgt tgcgccggcg ggtagatagg attccacgat ggccagctct
    48841 ttggtttcct tatccactag gtcctgccgc ccccctttgg cgaattgctc gatggactcc
    48901 ttccgctgtt tcacaagcgt ctgcaggaca tgaagcgctt cgccttcttc aagcggccgg
    48961 attttttcga tctctttgtt tttcagcgcg gacttgatca tgcgcaggac gctgagccgc
    49021 aattcgtccc tggccttcat cgcgcccgtc agctctttct gaattcgttc cgcgagactc
    49081 atcgcgttct cctgaggctt tcgactatag cagacggccg ctgcgcgtgg cacgagccgg
    49141 cggcgaattt gacatgctgt ggccgcgccg atagtttgga gctcggggat tgcggctttc
    49201 ccaggggcaa cacgaggagc ggcgtgaaaa tcgttgtcgc ggacaaaatc agcgagcgag
    49261 gggtcgaaat attgcgccag ccgggttgga acgttgtttt gcccggcaca agcgaactcg
    49321 aggccgaatt ggtgttggcg gacgccctta tcgtgcgcag cgcgacccgc gtgacgcccg
    49381 agctgctcga ggcagcgccc cggctccgcg tcgtgggccg cgccggcgtg ggcgtggaca
    49441 acattgatct cgacgcagcc acgcgccgcg gtgttctggt gatgagcacg cccggcggca
    49501 acgccgtcag cgttgcggag cacacctttg cgctgctgct ggcgatggcc cgccaggtgc
    49561 cccggctgga cgctgccatc cgcgccgggc gctgggagaa atcttcggcg gcgggaaccg
    49621 aactgcgagg caagacgctc ggtctcatcg gcctcgggcg gatcggcagc gaggtggcgc
    49681 ggcgggcgcg ggcctttgag atgcgcgtgc tggcgttcga cccgtacatc agcgaagcgg
    49741 cggcgcgcga agttcaagtg gagctggtgc cgctcgagcg gctgctggcg gagagcgact
    49801 ttgtctcgct gcacgcggcg ctgagcccgg cgacgcataa cctgatccat gccgggacga
    49861 ttgctcaaat gaagccggga gcgcggctca tcaatgccgc gcggggcgaa ctcgtggatg
    49921 aagctgcgct cgcggacgca ctgaagagcg gcaggcttgc cggggccgct ctcgatgtct
    49981 tcgccgaaga gccgccgaag aattcgccgc tgaccggcct gccgaacgtg atcgccacgc
    50041 cccacgtcgc tggcgccacc gaggaagcgc aggaagaggt cggcacgctc atcgcccagc
    50101 aggtgcgcga gttcctcgcc gaaggcctca ttcgcaacgc tgtcaatctt cccgcgctct
    50161 cggcggagca ataccgccgc gtgcgtcctt accttgaact ggcggagcgc ctcggctcgt
    50221 tcgtctcgca agcggccgcc gtgcgccccg cgcggattcg catccgctac gccggagagc
    50281 tggccgaagc cggcacgcac ctcttgcgca gcgcggtgct ggccgggatc ctcaacgccg
    50341 tcctcgatga gaaggtaaac ctggtcaacg cgtccgccgt ggccgcgtcg cgcggcctca
    50401 cggtggtgga agaaacgcgc cgccgcgagc acggtttccc gaacaccctg gaggtcgcgt
    50461 ctttaccgga gccggggagc gccacgaaag gcttctcggc ggagggcacg gtgctgcacg
    50521 atgcctcgcc gcgtatcctg cagattgacg gaatctctct cgaggggcaa ttggggggca
    50581 cgattctcta cgttcgcagc cgggatgagc ctggagtcat cgggcagatc ggcacgctcc
    50641 tcgggcaaca gggagtgaac atcgccacct tcgccctggg ccggcgcgag gggcgtggag
    50701 cggaagcggt gtcccttgta aggctggatg ggaacgtgtc ggactctgcc gtggagccga
    50761 ttcgaagaat acccgccgtg acggaagcga agctgctcca cctcgccaac gcctgagccg
    50821 ccgattgccg tccggctcat cctttgatga cgcccatggg gcgcaggcgc gcgactttgc
    50881 gcgcgagccc ggcgcgatgg accacgtcaa tcaccgcgtc aatatccttg taggcttccg
    50941 ggatttcttc caggatgccg tcccgcgttg cggcgcggag gataatcccc tgcgcctcaa
    51001 gcttttgcgt ctccacgcgt gcatcgcggc cgcgtttcgc agcggtccgc gacatcgccc
    51061 tcccggcgcc gtggcacacc gtgccgaaag tctcttccat cgcccgctcg gttcccacta
    51121 ggacgtaact cgccgtgccc atcgagccgg gaatgagcac cggctgcccc gcggcgcggt
    51181 acgcggaagg gatgaacggc gacccggccg ggaaagagcg tgtcgcgccc ttgcggtgta
    51241 cgaggacgtc cacctcgctg cgtccgacgc gatgccgttc gcgcttggcg atgttgtgcg
    51301 ccacgtcgta caccacgcgc agacgcacgt cttcgccgag caggcggcgg aacgcccctc
    51361 gggcgaaatg cgtaatcacc tgacggttgg cccaggcgaa attcgcggca gcggccatgg
    51421 cgccgaggta gctttgcccc tcgggcgatt gcgacggcac gcacgcgagc tggcggtccg
    51481 gcaatgtgat gccgtacttg cgcatcgctt cgttcatcgt cttcagaaaa tcggtgcaga
    51541 cctgatgccc caggccgcgc gagccgcagt ggatgagcac aacgacctga ttttcgcgca
    51601 ggccgaaggc ttccgccgtt tccggctcga agattttttc gacgtactgc acctccagga
    51661 agtggttgcc gcttcccagc gtgccgagct gcgtgcgccc gcgttgcttc gcgcgatccg
    51721 acacctgcga agggtcggcg ccgggcattg agccgtgttc ctcgcaatgc tcgctgtccg
    51781 cagcttcgcc gtagcccttc tcaacggccc aaaccgcccc ccgggcgaga acatcgtcca
    51841 gctccgcatg cgagcagggg accggacctt gcgatcccgt cccggaaggt acatcgcgaa
    51901 agagctggtt cacgagttcg cgcatcttcg gctgggcagc gtcgcgctcg acattgctcg
    51961 ccagcaggcg cacgccgcag ttgatgtcaa agcccacgcc ccctggcgag acaacgccct
    52021 cgcgcaggtt cgtcgccgcc actccgccga tggggaaccc gtaaccctgg tgcatgtccg
    52081 gcatggccag agagcgcccg acaatccccg gcagatacgc cacgctaatg gcctgctcga
    52141 tcgagaggtc gctcaggatc tgctccagca gcttttcgtc ggcgtagatc agcgcatcgg
    52201 tgagcatgcc ttcgcgcaag ctgcgcggca cgagccacct cacgtggtcc aatttctgca
    52261 gttgttgctt cgagattccc atggcgggca gtggcggcgc ggagcgcccc ggcacagcgc
    52321 gtctagccgc ctgcgacgca gctatgttag tctgttcgtc tagcttgggc cagtccttct
    52381 ggtctgggcg tgtccgtcgg ggcgtggctc agcctggtta agagcgtccc ggccgtgccg
    52441 ggaaggtcgg tggaaccgac gaccttccgc tggagttttg ccggttcctt cgtgtcgggg
    52501 cgtggctcag cctggttaga gcgcctggtt cgggaccagg aggtcggtgg ttcaaatcca
    52561 ctcgccccga ccatttcttt cgagcccata cgcacgcctt ctagcttttc gtccacggcg
    52621 ctgtaggcga ttcttctagt ctccatacga gcatctgtag gctcatccgg acgcttggtg
    52681 ctgaccttcc aaagttcctt aattcgggcc gtctgtacgg aacgggggga tagcgttggg
    52741 aaacaccttc ttgccgcgca gcagacaata ctccgcgtcc tgtattcgcc ccatttttcg
    52801 gtaagcatct gcaaggtgga cgtattcaat cgggttcccg gccgggaagt agcgcaccaa
    52861 gacttccagg tgcctgcgcg cctcctccca ccttttctca ctgaagtaca gttcgctgag
    52921 agcgctgcgc gcctctcccc tgtaagggtc tcgctcgagc aactcgacgt acaaacgttc
    52981 ggcgtctcga ttttcgccgt ttcgaataag caggtccccc aggaatagaa cctggttgaa
    53041 aggcctcgaa gggtcgccaa gaacgcgctt ggtttgccac gccagcgcct ccttccaatt
    53101 gccgcggcca ctttcaacca gagcgtatat ttcgaaagct ggcaaatacc cctgctggcg
    53161 ctcgagaatc tcccggagaa tacgctccga accgtcataa tccctgacgc ggagggccgc
    53221 tgcagcaagc cccatccgcg cggggatgga ggatgggtcc agccgcgccg cactggcgta
    53281 agcggcgcgg gcttcctcag gccttcttgc cgcaaccagc cagtctcctc gcagcagctc
    53341 agtttccgcc gtggccgagg cttttgccag ctcggaaata tagagcccgg cacgctctgc
    53401 atcttgcaga aacaaggaag tccgggctgc agccagcagc gattctcgtt tgtcccccag
    53461 gagaatggat gtgggaagca gggccgagcg cccctgcctg aacattcgca agttaaccgg
    53521 ggtggtgtcg tccccgaata ctgccagcgg agcttgatac tcgagactcg tcctgtcgtc
    53581 tgtgttgcgc gccccgttgc cgaccagccg ccgcaggtcg ccatcgtcca acatatgata
    53641 ggcaatcagc gcttcgggct cactgagccc gagcatcgcg taatcggagc gcagcgcggg
    53701 gacactccac agttgaccta ggcggtcgag gctgagctgc ccgggatcgc cctgtgagag
    53761 catcatcaga tcgcccagcg agccgcgcca cacgctcact tgagaaaagt gtggcgcgag
    53821 cgttcccaga atcatcttga cgtcctgcgg aaaaagctcg tatgcctgga cccactgcac
    53881 gaacactccc cccggcgcga gccgcgagcg cacttcctca tagaattcat ccgtgaacag
    53941 tgccgcgacg cccgcaatcc acgggttgga aggctcggag atgatgacgt catagcgatt
    54001 gcgggtggtg aaaagaaaat tgcgggcgtc atccaggatg atatgcaggc gcggatcgtt
    54061 caatacgccg cggttcaaat cctgaaggta cggcgctgcg tgaaaaaccg caggctcgat
    54121 ttcgacacaa tcgatctgct ccacttctga gtagcgcgcc accgctgaag ccgtcattcc
    54181 gcttccgaaa ccaatgacca gcacttttcg tggcgccttg tggaagacca tgcctagatg
    54241 gcccagcatc aactgcgaga cagagtccgc cgtcgaggca tccgtcttcc cattcgtggt
    54301 cagtcggaga ctgcctgagt cgcgctccac ggcaatcgtc gcgttcaagc cgtcttcgaa
    54361 gtaaagcaga tcggctgcat gtgcgatctg atcggcactc aggcgcgaag aatactgtcg
    54421 cgggtgttgc gccacgttga aattggcgat cgcaggatcg tagagtaatc gggaggctcc
    54481 cgccaccgcc acaatccccg cgagcgcgaa actccccgct agctcaacca ttcgccgggg
    54541 cctgctgcgc atgagtagaa agacagcaag cgctatgttg acaccggcgg caagcgccac
    54601 cgtgcgaaaa gcccccagcc gcggcacgag ccagaaaccc gtcgccacgc agcccaggat
    54661 cgccccgaca gtattcgcgg cacaggcttg tcccactccc ctcgagtacg ttgacccacg
    54721 ctcctctttg ctcgcgatta gcgaagtaac aagggggaaa ttgaatccaa aggcagtcgc
    54781 agcaggaagc atcgccagcc cgctgatcgc aaactgggtc agcaggagcc ctcggaaact
    54841 cttgtgggtc gctatcatca tccaccaggc catcacgggc attcgggcga acagaaccag
    54901 gaaaaccact gccccgattc ccgtgaatac ctgagtctcg ccaaaggtcg cgacggaaac
    54961 agtgttgttg cgccgcaccc aaatctcaaa cagccggctg ccgagcgcga ttcccgctag
    55021 gaacgtcgcc agcatgatcg taaatgagta ggtcgagctt cccagtgtgg tggccagcgc
    55081 ccgggtccac gaaatctcat acgccatggc cgtggctccc accagggcaa agctgaccag
    55141 cagaaacatg ggcctttcgt ctctgctccg aactggccct tcttgagtgc tctccaaagc
    55201 tggttgctcg ggagagcgcg caagtgccag ggccaagctc ccggcaagca cattgcaaac
    55261 tgctgccagc gccacggtca tgcgcaatcc cgcccaaggc agaaataaaa accctgctgc
    55321 gagcgcaccc aaaacggcgc ctgccgtatt gatccagtag agctgcccca gcttggctcg
    55381 cagttccgac gaactcttcc cgacgccagc cactagaatt ggcaaagttc cacccattag
    55441 aaaggtgggg agaaacagca cgaggatgga ccctgcgaat ctcagcgttt cgagaactgg
    55501 cacggaaccg gacgcaaagt gataagtggc tgcatacaag gcgcggacag cggtcaatcc
    55561 caacagggaa aggactccag tgattccaat gcctacctca atccacccgt atagcgtcac
    55621 cggacgcgcc tgtcgtccgc cccacttgcc cagataggcg ctgcccgccg cgagacccgc
    55681 cataaaagcg gctagaaccg cggtgatggc gtaaacagtg tgaccgaaga ccagtcccag
    55741 ggccttggtc cacgctacct cgtaaacgag acccgccacg ccggatagaa aaaaacagac
    55801 caggatgatg cgttcttgtg accgcgacaa tgtgctttcc cctctattca ggctaggggc
    55861 gctgctcggc ctggtcaagc cttcttcatt tttccatttc ttgggcttaa ctgcctgccc
    55921 tttttccttc ttgtattcag tctgagcaat acgtttcgcg gaattgctgg acggcagcac
    55981 gaactcaaac agcggaaaag aatctgaaca acgcttctta gcgcttgggt ccttgctctt
    56041 tttggtgctc cacgagaagg gcggctttgc cgatccgcac cgggtatttc acttcgcggg
    56101 tagcgcaccc tttggacagt gagagcctcc ggggtggcct acccttgcgg tttttgcaag
    56161 ggtgggtcac tcgtctcttg gagttcgcag cgaatggctc gtctgacgga gcttacgtcc
    56221 atggatcggc cacttgtttg cccgagaatt ccctccgcaa tggcgggtgg agcactcttg
    56281 agccgagaat ctgagtttgg gtggcgcacc ctttggaagt tggttttgca aagggtgtgg
    56341 gcttagcttc agcttcttga gcagcctggc caccatcaca tatcccggcg gctcgaccac
    56401 cagcctacga ttcgcgcggg cgacgaccgt cggtcaccga ccagatcggt cctctgccca
    56461 gcgcgggcaa cgaacaggca gagagtggct ggactgtggg cgattttgta gctatctgtg
    56521 cacccagttt ccacaattcc caacagagct caacaaacag gccgcaagtg attcatcaag
    56581 aaccgattac aaacaacaaa aatccataga tttcaataag gcttggcgcc tggttcggga
    56641 ccaggaggtc ggtggttcaa atccactcgc cccgaccact tttttagaat cagcgcctta
    56701 caacacacca agaacccgat gagcgcctgg tccgaaaccg tgcgttcttt tttcaaatcc
    56761 attgggcgcg agcgctcttt ctcctttgag ttcattgttt tgcaatagga ggttcgcttt
    56821 agtgggaatc cgattctcga ggaattaggc gcaaaatctg ccccttggga agaaaaatca
    56881 aagtccggtt caagtgtggc ttggtgagag gactagccgg ctgagtgtaa tcgtccggat
    56941 cgtttacttc gccggcttga tgttttgatt cacgcgaaac aggttcgccg ggtcgtagcg
    57001 cttcttaatc ttcgctagac ggtcgtaatt cttgccgtac gtcgcccgaa tgcgatcttc
    57061 accctcttcc atcatgaagt tgatgtacgc gccgccggca gaatatggat gaagctcgct
    57121 ccaatactcc ttcgcccacg tggagatttt ttccttgttc gcggggtcgg gatcgacccc
    57181 gaccatcacg gaggcccaca tcgcatcgcg gtaacaccac ggcgttgcgc cgttcttgac
    57241 gcggctggcc gcgccattaa tggggtacag gtgcatggtc gattgcatcg atggcaactt
    57301 cgccgcgtgc ttcagatgaa ccgcgatggc ttcgtcaggg agtgtgcgca cgaaatcggc
    57361 cttccaatac cattgcaggc ccggcggata gagaggatcg aacaaacttt gcagtaccgg
    57421 atgcggaatc gggccgacca tgtcgagcgc cggagcctta aagttccgga tcggcttgaa
    57481 caccttctcc gccttcttca tcggtccggt gtagcaccac acgatgccgc acatcttctt
    57541 gttgtgcaga tgctcgggga acggcggccc cggcggcacg gtgaggaacg caaagtaccc
    57601 atagacgtca tcgggcgcct tcgcgatgaa gctgcggtac cagcgcatca tctccgctga
    57661 gtcttccatc ggccacagca tcggcccgcc gtagaccgtg tgcacgggtt gcgccttgaa
    57721 cagaaacgac gtcacgacgc cgaagttgcc gcccccgccg cgcaccgccc agaacaggtc
    57781 ggtgttttcc tttccgctgg cagtaacgaa cctcccgtcg gccagcacca tttcgacggc
    57841 cagcaaattg tcgatcgtca ggccgtaccg ccgcgtcaag taaccgatcc cgccgcccaa
    57901 cgtcagtccg gccacgccgg ttgtcgagat gatgcccgca ggaaccgcga gaccgaacgc
    57961 gtgcgtcgca tgatccacgt cgcgccacag cgcgccgccg ccggccagca cggtccgctt
    58021 cttcgggtcc acgcggacgt agttcatcgg cgacaggtcg atcaccaggc cgtcatcgca
    58081 cacgcccagc ccggccgcgt tgtgcccgcc gccgcggatg gccaccagga gcttctgctc
    58141 gcgaccgaag ttcaccgccg tcatcacgtc ggccacgtcg gcgcaacgcg caatcaggcg
    58201 cgggcggcgg tcgatcatgc cgttgtacac cttgcgagcc gcgtcgtaac tcggatcgcc
    58261 tggctcgctc agccctccgc gcaggctggc tctgaagctg gtaatcgtcg cttcgttcag
    58321 catggttgcc ttcatccctg aaaatttgac ctgttcgatt aggatactag cccccaaaaa
    58381 cagtcttcag cgatcggaga tccatggcaa tcattctttt attctgggac atttttcgct
    58441 gaccggggca gcttcggcca atgtcttgaa gccgctccat ctgcatgcta gcatctgtgc
    58501 gcatcagggg acgccaatgc ccaaattcgt catcgaacgc gaagtcccgg gagccggaaa
    58561 tctatccgac acgcaattgc gggaaatttc ccaaaagtcg gtcaatattc tgaaggcaat
    58621 gggtcccgag atccagtggc tccatagcta cgtaaccggc gacaaggtgt actgcgtcta
    58681 cctcgctccg aacgaggccg ccatccaaga acatgccagg cgggtaggcc ttcctgccaa
    58741 tcgcgtctcc gccgtgcgcc gactcattga cccggagagc gcggagtaag cgctggctga
    58801 accttcctct gccttctgga cttccttcgc ccatcagggt cagcccgtcc gtacgggcgc
    58861 taacaggaag gagttgccgc ctcgggcatc agcacttcct caatagcttt ctcgaccggc
    58921 atagcccaac cttctaacca ggccgtcgcg cctgccgtgt tggtcaatgc ctgtcgtgcc
    58981 aggtgcaaaa tggcttccag tttggcctgt tccgcgggcg tgagcggggc gccaatattt
    59041 ttgcgcagcg cagccgctgc gcctgccagc cgcagcgaac gctctgcttc gaactgagca
    59101 gccgctgagc acgcaaaaca ctccagcagg cgggcaattc ctcgtttatg atccagttcg
    59161 tggaaaattt tcatgctttc ctggtacaga gagtgcgctg cgggataatc ccgttgttcc
    59221 ctcgccaagc tttccagatc agcgagcgtg ccagctatgc cccagcggtc accgagctct
    59281 cgg
//
LOCUS       DASYVO010000431         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_26687, whole genome shotgun sequence.
ACCESSION   DASYVO010000431 DASYVO010000000
VERSION     DASYVO010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26687"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VGR03_14830"
     CDS             <1..351
                     /locus_tag="VGR03_14830"
                     /inference="COORDINATES: protein motif:HMM:NF023134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEV1995603.1"
                     /translation="RRQKIAGEWVEPVNEILRITEGHDELVCADGIWRIEFIAREGGL
                     DWLLAAIARSAAEIVAEGPGARLRLCANPRCGLFFYDASRTHRRRWCTMAVCGNRNKV
                     AAFARKHGSSRRAQ"
     gene            complement(376..1764)
                     /locus_tag="VGR03_14835"
     CDS             complement(376..1764)
                     /locus_tag="VGR03_14835"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEV1995604.1"
                     /translation="MRRCVSFHRIVLVLCAFLSLTLVSQGSAKLVAKANAETTPAQGA
                     PLQPNPPQSPPSESKTTEQTKTEQYTLSHERYEKAVAYSRAGYTLYFVSYFLGGLFLF
                     LILRLGVAARFRDIAEKASDKRWVQGLLFVPLLWVTIDILDLPVQMYWHRLSLHYEQS
                     IQGWGSWFWDWTKGELLGIAFGIILVLGLFAVMRRNPRRWWLHFWPPAVLVLLGLIIA
                     TPLVIDPLFNKFEPLNKEHPDLVVSIEKLTKHAGVPIPPERMFLMVASQKTNAINAYV
                     TGLGVSKRVVIWDTTIQKTTNEEALFIVGHELGHYVLGHVRNGFLLGAVGLLLALYFL
                     FRGLHWTLDRWAGDWKIHGAEDWASLAVMLLLLQVLFFISSPVISGFSRMQEHAADVY
                     GLEVIHRLVPNSEEVAAHAFQVIGELDLADPNPPEFVKLWLYSHPPLADRLVFAHSYD
                     PWSKGEPPKYVK"
     gene            complement(1929..2426)
                     /locus_tag="VGR03_14840"
     CDS             complement(1929..2426)
                     /locus_tag="VGR03_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995605.1"
                     /translation="MPGEERLLNRPMIAPLALMVLLGASRIRAQESVAGTQIPPHDAP
                     AIVAKNKAKGLAQEHRFWDKKNGWLFVEVGASRTLDYFSTLNLRRRGDQEVLLTNDIV
                     DNHAAFAAVEAAGTGVSIGASYLFHRYGHHKLERWTSIVHIGLTTTGAVRNYCLKTAH
                     PKTSP"
     gene            2515..>2931
                     /locus_tag="VGR03_14845"
     CDS             2515..>2931
                     /locus_tag="VGR03_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995606.1"
                     /translation="MARYRLFLSSILMIAVAAGLSAYAGRMHAGASSGVPVRTFEFTY
                     QVHVPANPDTGTAHLWIPLPQPDGFQDINNLHIDSAVTYLRGRDPEYSNAFALFSPTA
                     QQSSAGYDVTMRFTATRREHKALLDGASIKNTSASAS"
BASE COUNT          626 a          909 c          782 g          614 t
ORIGIN      
        1 cgtagacaga agatagccgg ggaatgggtc gagccggtga acgaaatact tcgcattacc
       61 gagggacatg acgagctggt ttgcgcggat ggtatctggc gaattgagtt catcgcgcga
      121 gaaggcggcc tcgactggtt gttggccgcc attgcccgtt ccgccgcgga gatcgtcgcc
      181 gagggcccgg gagcgcgcct ccggctctgt gccaatcccc gatgcggcct tttcttctat
      241 gatgcatcac gtacgcaccg ccgccgttgg tgcacgatgg cagtttgtgg aaaccggaat
      301 aaagtggctg cctttgcgcg caaacacggc tcttcccgcc gcgcgcaata gtcctataac
      361 aggcttcgcc acgagctatt tcacgtactt cggtggctcc ccttttgacc acggatcata
      421 actatgcgca aacaccaggc ggtccgccag cggcggatgc gaatagagcc ataacttgac
      481 gaactccggc ggattcggat ccgctaaatc cagctccccg atcacttgaa acgcgtgcgc
      541 cgcaacctct tccgaattcg gcactagccg gtgaatgact tccaatccat acacgtccgc
      601 ggcatgttcc tgcattcggc tgaacccgct gatgaccggc gaggagatga agaacagcac
      661 ttgaagcagc aagagcatca cggccaggga agcccaatct tcggctccgt ggatcttcca
      721 gtcgccggcc caccgatcca gcgtccagtg caaacctcga aacaggaaat agagcgccag
      781 caaaagcccc accgccccca gcagaaagcc attgcggacg tgcccgagga catagtgtcc
      841 gagttcatgt cccacaatga acagcgcttc ttcgtttgta gtcttttgaa tggtggtatc
      901 ccagatcacg acccgcttcg acacccccaa accagtcacg taggcgttga tcgcatttgt
      961 tttctggctc gccaccatca ggaacatgcg ctccggcgga atcggaacgc ccgcgtgttt
     1021 ggtaagtttt tcgatcgaca cgaccagatc cggatgttcc ttgttcagcg gctcgaactt
     1081 gttgaacagc ggatcaatca ccaggggagt ggcgataatc aaacccagca ggaccagcac
     1141 cgccggaggc cagaaatgca gccaccagcg ccgcgggttg cggcgcatca ccgcaaatag
     1201 acccaggacg aggattattc cgaatgcgat ccccaggagt tctcccttgg tccaatccca
     1261 gaaccacgaa ccccagccct gaatcgactg ttcgtagtgg agcgagagtc tgtgccaata
     1321 catctgcacc ggcagatcca gaatatcgat ggtgacccat aggagcggaa cgaagagcag
     1381 tccttgaacc caccttttgt cgctggcctt ttcggcaatg tctcgaaatc ttgccgccac
     1441 gcccagccga aggatcagga acaagaaaag cccgcccaga aagtaagaaa cgaaataaag
     1501 cgtgtagcct gctcgcgaat aggcgaccgc cttttcatag cgctcatggg agagcgtgta
     1561 ttgctccgtc tttgtctgtt cagtagtttt tgattcgctc ggcggacttt gtggaggatt
     1621 cggctgtagc ggcgcgcctt gcgccggcgt tgtctccgcg tttgctttcg cgaccagctt
     1681 cgcggatccc tgcgaaacca aggtcaaaga caaaaacgca caaagaacga gcacgatacg
     1741 gtgaaatgag acgcaccgtc tcataagatc tcctcgcgtc tttccgaacg atgctgtccg
     1801 tgcgcctggg accaaggccc gcacgcgccg cgagtatgcc gcaccgtgtt cagaaccgca
     1861 acatttcatg ctgttgagta agatgagcgg aaacctggaa acgtttccat cccttgaaaa
     1921 tgccgcggct acggcgaagt cttcggatgc gccgttttca ggcagtagtt gcgcacggcg
     1981 cccgtcgtcg tcagcccgat gtgcacgatc gacgtccacc gttccagttt gtgatggcca
     2041 tagcgatgga ataaatacga ggctccgatc gaaactccgg ttcctgccgc ctcaaccgcc
     2101 gcaaacgcag cgtgattgtc cacgatgtcg ttcgtcagca ggacctcctg gtcgccgcgc
     2161 cgtcgtaggt tcaatgtcga gaaatagtca agcgtccgtg acgctccaac ttcgacaaac
     2221 agccagccat tcttcttgtc ccaaaagcga tgctcctggg ccagtccttt cgccttattc
     2281 tttgcaacga ttgcgggagc gtcatgaggc gggatctgcg ttcctgcaac cgactcctgc
     2341 gcacgaatcc tgcttgcacc cagcaatacc atcagggcga gcggcgcaat catcggacgg
     2401 ttcagcaatc tctcttctcc cggcaccctg tggtctccct tttgccccat gcctcaattg
     2461 agtataatcg cccggccgca tcttagggca ctctgtccgc gcaggagctc cccgatggct
     2521 cgctaccgtc tctttctttc ttcgattctc atgatcgctg ttgccgcagg cctctccgct
     2581 tacgctggtc gtatgcacgc cggcgcgtcc agtggagttc cggtccgcac gtttgagttc
     2641 acctaccaag ttcacgttcc ggcgaatccc gatacgggca ccgctcatct ctggattccc
     2701 ttgcctcagc ccgacgggtt tcaggacatc aataatttgc atatcgacag cgccgtgacc
     2761 tacctgcggg ggcgcgatcc tgaatacagc aacgctttcg ccctgttcag tccgacggca
     2821 cagcaatcat cggccggcta cgatgtgacc atgcgcttca ccgcgacgcg gcgcgagcac
     2881 aaggccctcc tggacggagc gtccataaag aatacttcag cgtctgcctc g
//
LOCUS       DASYVO010000432         3338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_191196, whole genome shotgun sequence.
ACCESSION   DASYVO010000432 DASYVO010000000
VERSION     DASYVO010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191196"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..422)
                     /locus_tag="VGR03_14850"
     CDS             complement(189..422)
                     /locus_tag="VGR03_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995607.1"
                     /translation="MREQVVRVECYSGYKADERPLRLNLTDGAREVVAIEDRWYSPGA
                     TYFRVLVDDGDRYLLRHEEAQDVWSLAGFRAAT"
     gene            complement(415..1257)
                     /locus_tag="VGR03_14855"
     CDS             complement(415..1257)
                     /locus_tag="VGR03_14855"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV1995608.1"
                     /translation="MNSAARLLRRAYRTFARVFPRCSVISQAIAFNLFLAFFPALLIA
                     VALATSRIGGRTSLFDLIADLTQFLPPGSQAIVSEFLVKRGPDAWKIAALGWTGTLLA
                     GSQVMKLVMQGIHAIYGDTEKLGFLHRQVRGLMLLLVTIAPLLGAAILGVLGKPLRRW
                     IAHEVGKHTMLQGWWNIFFPAAAMVLAMMALTVIYRVARPREDSMRNILPGSMVATLL
                     WWLVDVLFGIYVRRVPYSVVYGGLAAVIGLIIWMQLSAVIIFLGAAWNAELADIRRGT
                     RSHA"
     gene            complement(1422..2009)
                     /locus_tag="VGR03_14860"
     CDS             complement(1422..2009)
                     /locus_tag="VGR03_14860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1995609.1"
                     /translation="MMQENIHLRPAVPEDVPVLRELIEASVRGLQTQDYTPAQIEGAL
                     KTVFGVDSQLIADGTYIVAEAEPTAIERTEAENAPSQLMIVGCGGWSKRKTLYGSDHW
                     TGREDALLDPLGDAAKIRAFFIHPDWARRGVGSMILQACEDAARAAGFTRYEMGATLT
                     GAKLFGAKGYIAVEPISIPLVNGESLPVIHMEKQG"
     gene            2154..2426
                     /locus_tag="VGR03_14865"
     CDS             2154..2426
                     /locus_tag="VGR03_14865"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HEV1995610.1"
                     /translation="MLYMVIERFKNRDAKAVYRRFSEKGRMAPDGLAYVESWVETNFD
                     RCFQLMECGDVRLLEQWAAHWRDLVDFEFVPVRHSKEAVEIIAPEL"
     gene            complement(2428..2835)
                     /locus_tag="VGR03_14870"
     CDS             complement(2428..2835)
                     /locus_tag="VGR03_14870"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEV1995611.1"
                     /translation="MFMLNWANNPSDHGVRRASLRPVTSPSRRLTSRVPAPSDVWVYW
                     QCHGREDVSCVRDMSMGGLFIETPQPRAEGVLTRLHFLVQEGQIRADAVVRHAKPGVG
                     LGLKFTALSEQDRPKLAALLTRLRGTHHSREKS"
BASE COUNT          745 a         1013 c          920 g          660 t
ORIGIN      
        1 cgataggcag caagtaaata ctatccaaat gatgatgcgg aacccgggga atagcggtga
       61 atgaaacatc cctgcccttc agcaacccgc atcctaagcc gtccacgagc ccgcaaggga
      121 cgaaaccagg agatgctttg ctgttaactg gtgacttgac tatacgctgg acacaatttt
      181 tgtgtcaatc acgtagcggc tcgaaatccg gccaggctcc agacatcctg cgcttcttca
      241 tggcgaagca ggtaacggtc gccgtcgtcc acaagcacgc gaaagtaagt cgctcccggt
      301 gaataccaac gatcctcaat cgccacgacc tccctcgctc catccgtcag attcaacctc
      361 aagggacgct cgtccgcctt atatccggaa tagcactcca ccctgacaac ttgttcacgc
      421 atgggatcgc gtccctctgc ggatgtcagc cagctccgcg ttccaggcgg cgcccaggaa
      481 aataatcacc gcggaaagct gcatccagat gatcaagccg atcacggcgg caaggccgcc
      541 atacacgacg ctgtacggga cgcggcgaac gtaaattcca aacagcacgt ccaccagcca
      601 ccacagcagc gtcgcgacca tcgatcccgg caggatattg cgcatgctgt cctcccgggg
      661 ccgcgccacg cgataaatca ccgtcagcgc catcatggcc agcaccatcg ccgcggcggg
      721 gaagaagata ttccaccatc cttgcaacat ggtgtgcttg ccgacttcat gggcaatcca
      781 tcgccgcagc ggcttgccca gaacgccgag gatggccgcc ccgagcagcg gagcaatggt
      841 caccagcagc aacatcaggc cgcgaacctg acggtgcaga aacccgagtt tttccgtgtc
      901 tccatagatg gcgtgaattc cctgcatcac cagcttcatc acctgcgagc cggcgagcag
      961 cgttcccgtc catcccagcg ctgcgatctt ccacgcgtcc ggtccgcgct tcaccaaaaa
     1021 ttctgaaacg atcgcctgac tccccggggg caagaactgt gtgagatccg cgatcaggtc
     1081 aaacagactc gtcctgccgc caatccggct cgtcgccaac gccacggcaa tcagcagagc
     1141 tgggaaaaac gcgaggaaaa gattgaaggc aatcgcctgt gaaatcacgc tgcatcgcgg
     1201 aaatacgcgc gcaaacgttc ggtacgcccg ccgaaggagc cgcgccgctg aattcatagg
     1261 cgggcattat acctgcccga gccatctcgt gtctgaagcc actgagatgc accgcgatac
     1321 tcgagatctc ttgccgagaa tccgggcgcc tcgcgcaacg tctggtccag gaatgggagc
     1381 tggatgtgcc taggaaccag gcgataaatg cgggcctcgt ctcagccctg cttttccatg
     1441 tggatcaccg ggagcgattc gccgttcacc aatggtatcg agatgggttc cacggcgata
     1501 tacccctttg ccccgaagag ctttgccccg gtcagcgtcg cacccatctc gtagcgagta
     1561 aatccggccg ccctcgctgc atcttcgcaa gcctgcagga tcatgctgcc gactccgcgg
     1621 cgcgcccaat ccggatggat gaagaaagcg cgaatctttg ccgcatcgcc aagagggtcc
     1681 agcagggcat cctcgcgccc cgtccaatga tcgctcccat agagcgtctt gcgcttgctc
     1741 cagccaccgc aaccaacgat catcagctgg gatggcgcgt tctctgcttc cgtgcgctcg
     1801 atagcggtag gctcggcctc cgccacgata tacgttccgt cggcgatgag ctggctatcg
     1861 acaccaaaaa cggttttgag cgcgccttcg atctgcgctg gggtgtaatc ctgcgtctgc
     1921 aatccacgga cggacgcctc gatcagttca cgcagaacgg gaacatcttc gggaacagcg
     1981 gggcgaaggt gaatgttttc ttgcatcatg gttcgaaagc gggagccacg gagtggattc
     2041 tatcgccgag tggcggggga gcgcgacaga attcagattc accagcatta tgggctggta
     2101 tgttgaacga tgcgcaggcc gcgtcatcca caaaaggaag cgggtgatct ctgatgctct
     2161 acatggtcat cgagcgattc aagaatcggg acgcgaaagc ggtttatcgc cgcttcagtg
     2221 agaaggggcg catggctccg gacggcctcg cttacgtgga aagctgggtg gagacaaact
     2281 tcgatcgctg cttccagttg atggagtgcg gcgacgtgcg cttgctggaa cagtgggcgg
     2341 cacactggcg ggatctggtc gactttgagt ttgtcccagt gcgccattcg aaagaagcgg
     2401 tcgagatcat cgcgcctgaa ctctgaatta cgatttttcg cgggaatgat gcgtgccgcg
     2461 aagcctggtc agcaaagcgg caagtttagg ccggtcctgc tcactcagcg ccgtgaattt
     2521 caaccctaac ccaactccgg gttttgcatg ccgcacgacg gcgtcggccc tgatctgacc
     2581 ttcttgcaca agaaagtgca acctggtcag gacgccctcg gcccttggct gcggtgtctc
     2641 gatgaacaag ccgcccatgc tcatgtcccg cacgcacgag acatcctcgc gtccgtggca
     2701 ctgccagtac acccacacat cagagggtgc cggaacgcgg gacgtcaatc ttctggatgg
     2761 ggatgttact ggtctcaagg aggctctccg gacaccatgg tctgagggat tgttcgccca
     2821 attcagcata aacatgcgat atcacagtag tgtcaaagtt tccttaatcg tttcgccagg
     2881 aaagattcgg aggagatgat tcaacctccg ggcgattggt gtgatttgac cagaattgga
     2941 cagaggttcg atgcaattac catgaaacgc tggaccttgc aatctcacta cttggagatt
     3001 acgcgtgcgg ctccgtcccc tttcgcaagt gattaggtgc actcgcgtga aggtgcggtg
     3061 ttggagggga ctttcccatc agaacaaaag cttccggcaa gcggctcaac cgcacacgca
     3121 gaattcggct tccttgagac tcaattctaa gaaggagttt gctcgatgcc caatccaaga
     3181 atccacacga tcaagaaata tgggtgggtc cccgaccttc ccgaccatcg cgattttctt
     3241 tattccgctc cacaggccgt tctcggtgcg aaactcccga agagcgtcga tttgcgcccg
     3301 aaatgccctc ctgtctacga ccaaggccaa ctcggcag
//
LOCUS       DASYVO010000433         4763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_81371, whole genome shotgun sequence.
ACCESSION   DASYVO010000433 DASYVO010000000
VERSION     DASYVO010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81371"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VGR03_14875"
     CDS             complement(<1..230)
                     /locus_tag="VGR03_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995612.1"
                     /translation="MGNLVSISDSSTLIGTDQPATVRAPEIEAGVGCDFDSYLLVPDN
                     ALDDRIAAAKTRLGRDVVILGHHYQRDEVVKFA"
     gene            complement(316..1299)
                     /gene="moaA"
                     /locus_tag="VGR03_14880"
     CDS             complement(316..1299)
                     /gene="moaA"
                     /locus_tag="VGR03_14880"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEV1995613.1"
                     /translation="MTLHDKFGRQITDLRISITDRCNFRCVYCRSADPENYRDHDEIL
                     SWPELDRLARIFLSLGIRKIRITGGEPLVREGVEDYIARLHALGVEDLSMTTNGHLLA
                     ERCDRLLAAGLRRINISLDSLDPGRFERITRTKSFSTVMHSIDVAQKSRLAPAKVNAV
                     LVRGLNDDEVEAFALFARETGVIMRFIEFMPLDADRHWTRGLVVPAAEVHQRIHARWP
                     LEQIPHERSETARKYRFADGAPGEIGLIAPVTQPFCGHCSRIRLTADGKLRTCLFSKD
                     DHDLRALLREGASDEDLVTYISSIVMEKEEGHRINEPGFVQPSRTMVFIGG"
     regulatory      complement(1411..1557)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            1650..2831
                     /locus_tag="VGR03_14885"
     CDS             1650..2831
                     /locus_tag="VGR03_14885"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEV1995614.1"
                     /translation="MRIFVLGAGATGSLLAQLLERQGHTVWCGDRDPERARRFLGKKG
                     TIAVTEVNARNLRGIVKAAKGCQLIVNASASVFNEIVLRAALRLRAHYVDLSSHLTRH
                     PFRAEQFRYVKKFEEKNRVALINTGAAPGLTNLLVKRAADALDEVQAVHIRLYESSES
                     DDPISQWSPEVSFDEAVSHPQIYRSGKFQLGKRFSELEKFRFADPIGNVRVVLAAQDE
                     VATLPHFIPMRELDVKIGGNEIDRLRRWHKQGKLSRSRGMARHHFPQTSSPRTIAKLI
                     RQGILQNARFAAAVLVRGVTRGKKEDQHLLMRSDCTFPTLYQIRQQGRFTTPVAYATA
                     HVAALFIKFFPRDLAGVFAPEMLPTEARRAILAGIRNHNIRITHKTTVLKHTSEDEEE
                     F"
     gene            complement(2894..3637)
                     /locus_tag="VGR03_14890"
     CDS             complement(2894..3637)
                     /locus_tag="VGR03_14890"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEV1995615.1"
                     /translation="MRRMPRLFLLPLLALAASLIYFSSSAQEKSSKPDFDMTASYIEA
                     CSCDMFCPCYFNPHSTNHMGMGEHHMDEHFCRANLVLKVDKGFYKDTKLDGAKVWIAT
                     DLGSDWSTGKDSWLVVNFDPSVGKEQQAALGDILGQLYPIPWQKKAVDTAAFSWNVDT
                     KTGVARAKMDNGKGEVTLERVNGNDPHHEVVVHNLKYWGAQSNDGFRMWKSKHEAFEG
                     DGHKFSYEGTNGFLITIHFSGNAKPASAD"
     gene            complement(3808..>4763)
                     /locus_tag="VGR03_14895"
     CDS             complement(3808..>4763)
                     /locus_tag="VGR03_14895"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1995616.1"
                     /translation="LYKALASGGPQTPAELAKRTGTAERYVREWLSAQAAGGYVTYDP
                     ANGKFHLSEEQAFAMADESSPVFLPGAFQCALAAVKAEELLTERFKTGQGLGWHEHHH
                     ELFVGTERFFRPGYAANLVSAWIPALTGVDAKLKNGARVADVGCGLGASTVLMAKSYP
                     KSEFIGFDYHDKSIEMAKQRAKDAGVGDRIRFEVAKAKDYPGRDYDFVTFFDCLHDMG
                     DPAGASAHVHSTLKKDGTWMIVEPFAGDKLEDNLNPIGRAFYGASALICTPASLSQEV
                     GLALGAQAGEKRLREVVTSGGFSQFRRATQTPFNLIFEARP"
BASE COUNT          981 a         1348 c         1454 g          980 t
ORIGIN      
        1 gcgaacttga cgacttcgtc gcgctggtag tgatgcccca gaatcacgac gtcccggccc
       61 aggcgggtct tcgcagcggc aatgcggtcg tcgagcgcgt tgtccggcac aagaaggtac
      121 gaatcgaagt cgcaccctac cccggcttcg atttccggcg cgcggacggt ggccggctgg
      181 tctgtgccga tgagggtgct gctatccgaa atggatacga ggttgcccat ggattgtgtt
      241 tcgtctgcct agaggctgat gctaagcggt ccctgcctgg aagcttcatt ttctcatatt
      301 tcagcgaatt ttccactatc cgccgataaa taccatcgta cgcgagggtt gcacgaagcc
      361 gggctcattg atgcgatgac cctcttcttt ttccatcaca atcgagctga tataggtgac
      421 caaatcctcg tcggaggctc cttcgcgaag cagcgcccgc aggtcatgat cgtccttgct
      481 gaacaggcag gtgcggagct tgccatcggc ggtgagccgg atgcgggagc aatggccaca
      541 gaatggctgg gtcactgggg cgatcaggcc gatttcgcca ggcgcgccgt cagcaaagcg
      601 gtatttgcga gcggtctcgc tgcgctcgtg gggaatctgc tcgagcggcc agcgggcgtg
      661 gatgcgctgg tggacttcgg cggcggggac gacgaggccg cgcgtccagt ggcggtcggc
      721 gtcgagcggc atgaattcga taaagcgcat gatgacgccg gtttctcggg cgaacaaagc
      781 gaaagcttcg acttcgtcat cgttgaggcc gcggaccagg acggcgttga ctttggcggg
      841 cgccaggcgc gatttctgcg cgacatcgat gctgtgcatc accgtcgaaa aagacttggt
      901 tctggtgatg cgttcgaatc gcccgggatc gagcgaatcc agactgatat tgatgcggcg
      961 gaggccagca gcaaggagcc ggtcgcagcg ttcggccagc aggtggccgt tggtggtcat
     1021 ggagagatcc tcgacgccga gggcatggag acgcgcgatg tagtcctcga cgccttcgcg
     1081 gacgagcggc tcgccgccgg tgatgcggat tttgcggatg cccaggctga ggaagatccg
     1141 ggcgagacgg tcgagctcag gccaggagag aatttcgtcg tgatcgcgat agttttccgg
     1201 gtcggcagag cgacagtaga cacagcggaa attgcaacgg tccgtgatgg agatgcgcaa
     1261 gtcggtgatc tggcggccga atttgtcgtg aagggtcatg gtccgatgtg cttcttggat
     1321 gcagctcccc gctacctgga cgcgctagcg ttctaaaaca aaaccgcccg atccagtgct
     1381 gggaatcggg cgctcaccga aaaactgcac attttcggcg tgatcgctcc tttccaagca
     1441 cgggaggccc gggcgtttcc gcccaaaccc tcagccccga taatttctcg ggacatcgcc
     1501 ccagcagccg tgggttatct ctcccgtagg cttcaggggt cggagttaca gccatagtct
     1561 acgccgcctc cctgaagtac gcaattggcc caccggaaaa ccccggctga ggtaggccct
     1621 tcggattgtg atagagtttt ggccacgcca tgcggatctt tgttttaggg gccggagcga
     1681 ccggctcact tctcgcacaa ttgctggagc ggcaaggaca tacggtctgg tgcggagacc
     1741 gcgacccgga acgcgcgcgg cgcttcctgg gcaagaaggg caccatcgcg gtaacggaag
     1801 tgaacgcgcg aaacctgcgc ggaattgtga aggccgcgaa aggctgccag ctgatcgtca
     1861 acgcttcggc ttcggtcttt aacgagattg tgctgcgtgc cgcgctgcgg ctgcgcgcgc
     1921 attacgtgga tctcagctcc cacctgacgc gccatccgtt cagggccgag cagttccgct
     1981 acgtaaagaa attcgaggaa aaaaaccgcg tggcgctgat caataccggg gctgcgcccg
     2041 gtctgacgaa tttgttggtg aagcgcgcgg cagatgcact ggatgaggtc caggccgtgc
     2101 acatccgctt gtatgaaagc agcgagagcg acgatccgat ttcccagtgg tcgccggaag
     2161 tatcgtttga cgaagcggtc tcgcacccac agatttatcg cagtggaaaa tttcaactgg
     2221 gcaaacgatt ttccgaacta gagaagtttc gatttgccga tcccatcggc aacgtgcgtg
     2281 tggtgcttgc ggcgcaagat gaagtggcca cgctgcctca cttcattccc atgcgggagc
     2341 tggacgtaaa aatcggcggg aacgagatcg accgactgcg gcgctggcac aaacagggaa
     2401 aactgtcccg gtcacgcggg atggcccggc atcactttcc tcagacgtct tctccgcgca
     2461 cgatcgccaa actgattcgg caggggattc tacagaacgc tcgtttcgct gcggccgttc
     2521 tggtgcgggg agtgacgcgc ggcaagaagg aagatcaaca cctactgatg cgcagcgatt
     2581 gcacgtttcc cacgctctat cagattcggc agcaagggcg atttacgacg ccagtggcct
     2641 acgcgacggc gcacgtcgcg gcgcttttca tcaaattttt tccgcgggat cttgccggcg
     2701 ttttcgcgcc ggagatgctg cccaccgaag cccgtcgcgc catcctcgct ggaatccgca
     2761 accacaacat ccggatcacg cacaagacaa ccgtactaaa acacacaagt gaagacgaag
     2821 aagaattcta gccagtcaag cgcgctttgc cagaaagcaa agcgcggctg actggaccga
     2881 ttcgagcaga ggatcagtcc gcagaggcgg gctttgcatt tccagaaaag tggatggtaa
     2941 tcaggaaccc attcgtccct tcgtaggaaa acttgtggcc gtccccttcg aaggcttcgt
     3001 gcttgctctt ccacatgcgg aagccgtcgt tggactgtgc cccccagtat ttcaagttgt
     3061 gcacgacaac ttcgtggtgg ggatcattgc cgttgacgcg ctcaagcgtg acctcgcctt
     3121 tgccgttatc catcttggcg cgggccacac cggtttttgt gtccacgttc catgaaaacg
     3181 cagcggtatc gactgccttc ttctgccacg gaatcggata gagttggccg agaatgtcgc
     3241 caagtgcggc ctgctgctcc ttgccgacgc tgggatcgaa gttcaccacc agccaggagt
     3301 ccttgccggt actccagtca ctgcccagat cagtggcgat ccagaccttg gcgccatcca
     3361 gcttggtgtc tttgtaaaaa cccttgtcca ctttgaggac gaggttggca cggcaaaaat
     3421 gctcatccat atggtgctca cccatgccca tatggttcgt ggagtggggg ttgaagtagc
     3481 agggacagaa catgtcgcag ctgcaagctt cgatgtaact ggcggtcata tcaaaatcgg
     3541 gtttggaaga tttctcctgc gcggaagaac tgaaatagat gaggctagcg gcaagcgcaa
     3601 ggagcggaag caaaaacaga cgtggcatac gtcgcattta accctcctga ggaaatgtga
     3661 agtgcgggcc caaccgggaa tagcgcgagt gtactctcac atcgtggtcg attcaagggg
     3721 cccaatccta aatatagcag ttagggatgg gaggggcata attacggtcg aggggtgcga
     3781 ttctaggccc cgctcaagct gcagatccta cggccgggct tcaaagatca gattgaacgg
     3841 tgtctgcgtg gcgcggcgga attgcgagaa gcctcccgag gtcaccactt cgcggagccg
     3901 cttttctccc gcctgcgctc cgagcgccag acctacttcc tgcgacagtg aagccggcgt
     3961 gcaaatgaga gccgacgcac catagaaggc acggccgatg gggttgagat tgtcctcgag
     4021 cttgtcacct gcgaagggct cgacgatcat ccaggtgccg tccttcttca gtgtgctgtg
     4081 cacatgtgcc gaagcgccgg caggatcgcc catatcgtgc aggcagtcga agaacgttac
     4141 gaagtcatag tccctgcccg gataatcctt cgccttggca acttcaaaac gaatccgatc
     4201 gcccaccccg gcgtctttcg cgcgctgctt cgccatttcg atggacttgt cgtgatagtc
     4261 gaaacctata aattcagact ttgggtaaga tttagccatc aagacggtcg atgcgccaag
     4321 gccgcaaccc acatcggcga ctcgcgcgcc atttttcagt tttgcgtcaa cgccggtcag
     4381 cgcggggatc caggcactga cgagatttgc ggcataacct ggacgaaaga agcgttccgt
     4441 gccaacgaag agttcgtgat ggtgttcgtg ccagcccagg ccttgcccgg tcttgaatcg
     4501 ttccgtcaat agctcttccg ccttgaccgc ggcgagggcg cactggaaag ctcccggcaa
     4561 gaagaccggg ctggattcgt cggccatcgc aaatgcctgc tcctcactca gatggaactt
     4621 gccgtttgca ggatcgtagg tgacgtagcc gcctgccgct tgtgcgctca accattcgcg
     4681 cacataacgt tccgcagtcc ccgtgcgctt ggcgagttcc gcgggagtct gcggtccgcc
     4741 ggacgcgagg gctttatata agc
//
LOCUS       DASYVO010000434        18825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_207634, whole genome shotgun sequence.
ACCESSION   DASYVO010000434 DASYVO010000000
VERSION     DASYVO010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 18825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18825
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207634"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VGR03_14900"
     CDS             <1..417
                     /locus_tag="VGR03_14900"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEV1995617.1"
                     /translation="YICYDRHFPEGARILGLNGAEIVYNPSATVAGLSEYLWELEQPA
                     HAVANAYFVAAINRVGIEHPWKIGEFYGKSYFCDPRGKIVKQASRDKDELLVAELNLD
                     EIQKVRDTWQFYRDRRPESYGEITRTRAGENSEAAN"
     gene            419..1135
                     /locus_tag="VGR03_14905"
     CDS             419..1135
                     /locus_tag="VGR03_14905"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEV1995618.1"
                     /translation="MLRAETIESSSLYNKDLAPVSSERRTWRTYNYAALWISMSVNIP
                     TYMLASGMIAGGMNWKQALFTVFLGNVLVLIPMLLNAHAGAQYGIPFPVFARASFGVL
                     GANVPAILRALVACGWFGIQTWIGGEAINAMIVALAPAWANVHYGAAVCFFFFWLLNV
                     LVILRGIETIRFLQGISAPFLLLIGLALLLWARSKAGGFGPHACHALEVSNLRRILPL
                     LHPFADRRRRFLGHRFAEHP"
     gene            1023..1832
                     /locus_tag="VGR03_14910"
     CDS             1023..1832
                     /locus_tag="VGR03_14910"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEV1995619.1"
                     /translation="MLATPSKFQTFGEFFRFFIPSLTGVVGFWATVSLNIPDFTRYAR
                     SQRDQIVGQALGLPATMTFYSFIGIAVTSATLIIFGEALWNPVAVLARLGNPWAVVLA
                     MIALLMATLNVNVAANVVSPANDFSNLSPRRISFRTGGLITCAMGIVMQPWKLMADYG
                     SYIFGWLVGYSGFLGPIAGVLICDYFVVRKKVLIVEDLYQRGGRYEYARGFNWTAVAA
                     LAAGAGIAFVGLVVPPLRVLYNYAWFVGFLVSFLAYFALMSRVAPVTQPAN"
     gene            1848..2843
                     /locus_tag="VGR03_14915"
     CDS             1848..2843
                     /locus_tag="VGR03_14915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEV1995620.1"
                     /translation="MDFGITIKPDISIDRILSLTRHAESSGFSFGWIFDSHVIWKEPF
                     PLLTLMAANTKKMRLGTCVTNPAVRDVTVSASLFATLNLASHGRMELGIGRGDSSRRV
                     LGKKPSTLENLEEFVRIFRNLNAGNTVDLEGVPTKFSWTNGEIPRVWVAGYGPKALRT
                     AGRIGDGVILQFADPDLIDWCLGFVREGAKEAGRDFSKIEVMAAAPVWASDDLKAARE
                     HVRWFPALVSNHVMDLISKYKPEELPPALTSFVKDRGKYDYLHHCEVGSDNAEFVTDE
                     VVDRFCIIGPIDEHRKKLDRLRKVGVTQFNIYLMCGDEEKTLDGYQHKILPHFKH"
     gene            3367..4758
                     /gene="hydA"
                     /locus_tag="VGR03_14920"
     CDS             3367..4758
                     /gene="hydA"
                     /locus_tag="VGR03_14920"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEV1995621.1"
                     /translation="MRFDTVIRSGTVVTATDTCVADVGINGDKVTAIAAQLPTENATR
                     ITDATGMLVIPGGIDVHTHLDMPFGGTTSADDFQTGTIAAAFGGTTTLIDFAIQYKGQ
                     TLRQAFDTWMKKAHDKAVTDYAFHCIITDLGSAQLEEMGQLVREGVTSFKLFMAYPGI
                     FMLDDATIFKAMRQAAKHSALICMHAENGGAIDVIVQQALAEGKRAPKYHALTRPTTA
                     EAEATSRAIALAEMAGAPVYIVHLSCNEALEKVREARDRGLPAYAETCPQYLYLSLEN
                     MDAPGFEGAKYVFTPPLREKWHQEKLWQGLAKDTLQVVSTDHCPFCFKEQKELGKDDF
                     TKIPNGGPGIEHRLSLVYTGGVHGKHFSPNRFVQLVATAPAKLFGLYPRKGTIAVGSD
                     ADLVIFDPNHEEVISAKTHHMRVDYSMFEGIKTKGAPKTVFSRGRAVIDAGKFVGRPG
                     AGQFLRRQTYAGI"
     gene            complement(4788..5255)
                     /locus_tag="VGR03_14925"
     CDS             complement(4788..5255)
                     /locus_tag="VGR03_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995622.1"
                     /translation="MRETVLRDFLLGRVTATALARDILGSTKRVEPIKFLVEIEDMDE
                     EFWISREMLVSLCEAVLSAELPPQELSTIGFALVASGKFAWDAEDLCGHVIHDWSCPE
                     VNYPLTIENVQRFKNWLLELEPYPAKPQLGPPTKNECLISITEKKSLPRQQRG"
     gene            5482..8355
                     /gene="uvrA"
                     /locus_tag="VGR03_14930"
     CDS             5482..8355
                     /gene="uvrA"
                     /locus_tag="VGR03_14930"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV1995623.1"
                     /translation="MGLTKLVIRGARQHNLKNISVEIPRNSLTVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLDQMERPEVDVIEGLSPSIAIEQKTTTRSPRSTVGT
                     ITEIYDYLRVIYATVGVPHCPNCGKSITRQSSEQIVQSILHGEICKPDDRIMILAPIV
                     RGRKGAYRKELEKFAQDGYVRFRLNGELFPLDDLPVIDKRKNHTIEIVIDRLLVKQGI
                     AARLEQSIATALKLASGLVTVAIVGGKEHTFSEKLACPDCGISVPQLEPRSFSFNSPY
                     GACPACNGLGSKYDFDPSKIVTDWTRPLFDGGLGPGSGSAILKRTLELGAYAHGFDLE
                     TPFEKFPARVQNMLLYGYPPSNGSGSGENTSGEKKPKGEKSFRFPGILKFLERNFEES
                     NSDSYREWITQYMSATLCGTCHGRRLRPESLAVKLASWSLSDFTALSLSAARPAVAKI
                     LTHLTERQKEIAARPLEEVSERIDFLLAVGLGYLSLDRSAATLSGGEAQRIRLATQIG
                     SRLRGVLYVLDEPSIGLHARDNARLLGSLEQLRNLGNTVLVVEHDEDTIRRADFVVDL
                     GPGAGNAGGYLVAQGKPAQIEATPESLTGQYLSGAAKIAVPEKRRSPNGKAIQILGAS
                     ANNLKDVDITLPLGLLTVVTGVSGSGKSTLVNDILYRALAQKLYRSSEAPAAHREILG
                     ADQIDKVIEIDQAPIGRTPRSNPATYTGVFAPIRDLFAMLPESRERGYRPGRFSFNVK
                     GGRCEACQGDGLRRIEMNFLPDVYVMCEVCRGRRYNSETLAVRYKGHSISDVLNLPSS
                     DALKVLENIPQIQQKLATLVEVGLGYVTLGQSATTLSGGEAQRIKLARELSKRQTGRT
                     LYILDEPTTGLHFDDVRKLLDVLQRLVDLGNTVLIIEHHLDVIKQADWIIDLGPEGGE
                     GGGRIVAQGPPELVARTKKSYTGQVLARVLGISNNGHHNGNAK"
     gene            8470..9474
                     /locus_tag="VGR03_14935"
     CDS             8470..9474
                     /locus_tag="VGR03_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995624.1"
                     /translation="MSIYSIQPITLGAVHTYPLGSRKSKVSVRDFAKPPNAKTSLTKF
                     LDSLPNILAAEDLRHLLAAIHTARRQRRAILWGIGGHVIKVGLGPVLIDLMKRGFVSG
                     IAMNGAALIHDFEIALAGNTSEDVEAGIGEGRFGMAAETGQYINEFAKLSHRVRIGYG
                     EAAGQFLSSGIIEVKHADSSVLVAAYKHRIPVTIHLAIGTDIPHMHPAANGAALGDAT
                     HHDFRLFCALVQQMHPGGVYLNWGSAVLLPEVFLKAVSVVRNLGVPLRPITTANFDFI
                     QHYRPLQNVVKRPTASAPSRSGPESHGYAITGHHELLLPLVAAALAADWPKKRRARKR
                     "
     gene            9471..10463
                     /locus_tag="VGR03_14940"
     CDS             9471..10463
                     /locus_tag="VGR03_14940"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEV1995625.1"
                     /translation="MSLFDDNPPNHTEENLPAMSSPDQPEQSASAKPFAQPFGGDPLS
                     YVAVTPAASIPSRNLPEDLRISWSWPHLVVFLIFGFGSLLLVQIGAVLLLSANKHLSQ
                     KQLQQMIESKPQFLVGTNVLWYALLFLFLYMTLAVLRGLPFWRSLGWKKLRADPAIGK
                     GGPWMYFFSGCGLAIFVAIASSRVKNADHVPIQELFKSRSGAMLLMAMAVFVAPLVEE
                     TVFRGYLYPLFASKLSGLAQNAGVNPLRAIRFGTFAGIFITGILFGLMHGAQLGWTWG
                     LVSLLTLVGVLFTFARAWTGTVLASFLLHLGYNSMIAITSIIATKGFTHMPPHP"
     gene            11128..12198
                     /gene="mtnA"
                     /locus_tag="VGR03_14945"
     CDS             11128..12198
                     /gene="mtnA"
                     /locus_tag="VGR03_14945"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEV1995626.1"
                     /translation="MYVAQPIEWTGQGVVMLDQRRLPVEEISYTYTDYREVAKAIREM
                     VIRGAPAIGVAAAMGAALGVLHSSAKSVEALRAEFTEICDALAKTRPTAVDLFWALER
                     MKRRFAELTAENSDLGRIKQKMVEEAQQIHLEKRATDEAIGRFGAEFMPREGQVMTQC
                     NAGALATGGIGTALGVIRVAFEQGKKLHVLVPETRPYLQGARLTAWELHKGGIPLTLI
                     TDNMVGHFLKTGKVGAIVTGADRIAANGDTANKIGTYQIAVLAKENNVPFYIAAPVST
                     FDLSIPNGEHIPIEERSAAEVTHFQGIRIAPDVHAAHPAFDVTPARYIAAIFTERGVA
                     RAPYTDALRALASLSQSASIAR"
     gene            12270..15710
                     /locus_tag="VGR03_14950"
     CDS             12270..15710
                     /locus_tag="VGR03_14950"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV1995627.1"
                     /translation="MLSWSRPLARAFILVLACILVPSLHSSPQPSWSGVLRDSAGNPV
                     EMATIKLFAAESNREFSATTYATGQFAFTAVAPGSYRLTVSAAGKIWTAADPVVIKDG
                     ATLTSGLQLSLQGQEPRLELRIMVVQDAASPQASGGEHLSTGEVSSLPLNERDFSKLL
                     LLAAGTMTDSNGAANFTQQFAVNGQRGSAAVFAIDGADTTDPELGGATFSNFNVDAIQ
                     EVQSISGVMPADTGHGAAGFTNVVTKSGSNQVHGSLFEFVRNASFDARNFFDHKSDVD
                     TRHLPPFARNEFGVTNGGPVFVPRIYDGRGKTFYFAEYQGFRQVLGTTQVIPVPTLAE
                     KQGIDTATFPGDTLIVPVNSSIAPLLARYPLPNDPQGAYGARTYATSSKVVTRTDQAS
                     FRVDHRLSEKSTLFLRVSRNQVTGPTTNPDQTVTDPDFGVKFLDHQRNAAIKYSRTPT
                     PHFSYQTTLGFIRSTPLFITSNHTQPAIAFGDGLFQGFNSADGSIFGSYGNVYQFKHD
                     MVYTRGSHSFKWGAELRFNRDSTIFGLNPNGLYTFGGGTAYSPVLIPSASGQHDIHPG
                     DPLPDSLTGLLTATPYSYTVTAAASLTPKGDKFDEAAVRREAYNFYFLDTWKATARLS
                     VNYGLRYEVNSRIHEAKHRTSLAIPIGPNGQPADFLTPGTKQIYVYNPQPPYAMDWNG
                     WGPRLSVDYGLTKHTTLHAGGAITTILPNLWLENYVTGAFPFIFQPLVTALPGFPLSF
                     QDAVVPLTLPPVYTTQGKLLFSSGSSSNVAPNTPIDVQKFQNDLAALTPGNQVQLFSA
                     ISFDPHSRNGYIATETLGIDHEIGSVKLSASYVGTVGVHLASVLSPNGYGGADPAFAP
                     YTQFNSAGHAIGGFGPESVMTTGSHSSYHALQTSVSQNTARMGLSFQASYTFSKSIDD
                     TSAVPGGISGSPGVTLQALPQDPFNPKADKGPSTFDVTHVFTLSLMEALPLDRVRFLQ
                     PLGKTLTKGWQLLNITTLTTGPPFTVYSGVQQTGAGAGGTDRPDLLAMPNFSTSRTVR
                     EDYFGMGAANDTFFQIPINIPGGTGPNQGRFGTLGRSTFRGPGFRDFDMALIKNTSFG
                     HRGNGELAILQFRAEFFNIFNEVNFGLPSNIVLGSGFGVISKTAGPSRQIQFSLKLNY
                     "
     gene            complement(15704..16123)
                     /locus_tag="VGR03_14955"
     CDS             complement(15704..16123)
                     /locus_tag="VGR03_14955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEV1995628.1"
                     /translation="MGFAAFAGHKYLNLETFKKSGDGVKTPVWFAADPASDLASDAAR
                     LYAYTIGNSGKVKRIRNNGRVRIAPCDMRGNLLGEWVDARAEIVTGADAEYGNRLLNK
                     KYAPWKQLLNFFASFRRRGRTGFVIRPVGNEARTKGQ"
     gene            complement(16254..16769)
                     /locus_tag="VGR03_14960"
     CDS             complement(16254..16769)
                     /locus_tag="VGR03_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995629.1"
                     /translation="MRSMWKILGVLVLSMGIMPMPNSAAGSGAEAFEKLKALVGHWET
                     DKTNMNKATLDLELTSGGTAVLEKFRMVESGKPVEMTTLYYLDGDQVKLTHHCMAGNQ
                     PTMKGSYAPETKTITFDLVSISNLKSADDGHMHHAVYTFIDNDHFKTTWTFRKEQKDA
                     FTEDVTYVRTK"
     gene            complement(16854..17222)
                     /locus_tag="VGR03_14965"
     CDS             complement(16854..17222)
                     /locus_tag="VGR03_14965"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1995630.1"
                     /translation="MSRVIHFEIPASDPERASAFYQKVFGWKFDKWAGPMEYWLVTTG
                     AQGTPGINGGMLKKPGPVSVTTNTIDVESVDAAIAAAVKAGGKQMMPKSPIPGIGYFA
                     YCEDTEGNLFGVMQADKNAK"
     gene            complement(17277..17642)
                     /locus_tag="VGR03_14970"
     CDS             complement(17277..17642)
                     /locus_tag="VGR03_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995631.1"
                     /translation="MNKVLLGLLLGTVLGAVDGGTAWFTPEVRPEMAMIIIGSTFKGL
                     VGGVLIGFFSRKVNSLPLGIVFGLAIGALLAFAIAAMPDGITGKHYYAQIIIPGSLVG
                     LIVGYATQRYGASPAPATR"
     gene            complement(17707..18744)
                     /locus_tag="VGR03_14975"
     CDS             complement(17707..18744)
                     /locus_tag="VGR03_14975"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV1995632.1"
                     /translation="MRFLSDSALERLRDGAEAPDLAGTRYRLLERIARGGMGVVYAAE
                     DENLQRRVALKVLDVPGTDGDLTNRLIREARVLAALEHPGIVPVHDVGTLADGRVFYT
                     MKFVEGKRLDKYIESVASVPNRLRIFLRICDAVAFAHARGVLHRDLKPANIMVGPFGE
                     ILVMDWGLAKILHGEVSREVRGADSEATVFEKPKQPAFASDAREISVVTGHGTVMGTP
                     GYMSPEQARGDVEHLDARSDIYSLGALLQFLLTGHVEPAPIPTGRRLDKSLAAICAKS
                     TAASPAERYPSVQEMALDVSRYLDGLAVGAHRETVFEKAGRFYRRYRFFILLIAAYLA
                     MRVLILLFMHR"
BASE COUNT         3840 a         6372 c         4645 g         3968 t
ORIGIN      
        1 tacatctgct acgaccgcca tttccccgaa ggcgcgcgca tcctcggcct caacggcgcc
       61 gaaatcgtct acaacccatc cgccaccgtc gccggcctct ccgaatatct ttgggagctc
      121 gagcagcccg cccacgccgt cgccaacgcc tatttcgtcg cggccatcaa tcgcgtcggc
      181 atcgagcatc cctggaagat cggcgaattc tacggcaaga gctacttctg cgatccgcgc
      241 gggaaaatcg ttaagcaggc cagccgcgat aaggatgaat tgctcgtcgc ggaattgaat
      301 ctcgatgaaa ttcagaaagt gcgcgatacc tggcagttct atcgtgaccg ccgcccggaa
      361 tcctacggag aaatcacgcg cacccgtgcc ggagaaaatt ccgaggcggc caactaaatt
      421 gcttcgcgcg gaaaccatcg agagcagttc gctctacaac aaggacctcg ctccggtctc
      481 cagcgaacgc cgtacctggc gcacttacaa ctacgccgcc ctgtggattt ccatgagcgt
      541 aaacattccc acctatatgc tagccagcgg aatgatcgcc ggcgggatga actggaagca
      601 ggcgctcttc accgtgtttc tcggcaacgt gctggtgctg attcccatgc tgctcaacgc
      661 tcatgccgga gcgcaatacg gcatcccgtt cccggttttt gcacgcgcct ccttcggcgt
      721 cctcggcgcc aacgttcccg ccattctgcg cgctctcgtc gcctgcggct ggttcggcat
      781 tcaaacctgg atcggcggcg aagccatcaa cgccatgatc gttgcccttg cgccggcttg
      841 ggcaaatgtt cattatggcg cggcagtctg ctttttcttt ttctggcttc tgaatgtgct
      901 ggtcattctg cgcggcatcg aaaccatccg cttcctgcaa ggcatctccg ctccgtttct
      961 gctgctcatc gggctcgccc ttctcctctg ggcgcgcagc aaagccggcg ggttcggccc
     1021 ccatgcttgc cacgccctcg aagtttcaaa ccttcggcga attcttccgc ttcttcatcc
     1081 cttcgctgac cggcgtcgtc ggtttctggg ccaccgtttc gctgaacatc cctgatttca
     1141 cccgctacgc tcgcagccag cgcgatcaaa tcgtcggcca ggccctcggc ctccctgcga
     1201 cgatgacttt ttattccttc atcggcatcg ccgtcacctc cgccaccttg atcatttttg
     1261 gcgaagcgct gtggaatccc gtcgccgtcc tcgcgcgcct cggcaatccc tgggcggtgg
     1321 ttctggcgat gatcgcgttg ctcatggcca cgctcaacgt caacgtcgcc gcaaacgtcg
     1381 tttccccggc gaacgatttt tccaatctct ctccgcgccg catcagcttc cgcaccggtg
     1441 gcctgatcac ctgcgccatg ggtatcgtca tgcaaccctg gaaattgatg gccgactacg
     1501 gcagctacat tttcggctgg ctcgtcggct actccggttt tctcggcccg attgccggcg
     1561 ttctcatctg cgactatttc gtcgtccgca aaaaagtcct gatcgtcgag gatctttatc
     1621 agcgcggcgg ccgctacgaa tatgcgcgcg gcttcaattg gacggccgtc gcggcgctgg
     1681 ccgcgggtgc aggcatcgct ttcgtcggtc ttgtagtccc gccgttgcgc gtcctgtaca
     1741 actacgcctg gttcgtaggc ttcctggtaa gcttccttgc ctacttcgca ttgatgagcc
     1801 gagttgcacc ggttactcag ccggcgaact gaggaggagc attttccatg gacttcggca
     1861 tcaccattaa acctgacatc tccatagacc gcatcctgag cctcactcgc cacgccgaat
     1921 cctccggctt ttccttcggc tggatcttcg attcccacgt catctggaaa gagccttttc
     1981 cgctcctgac actgatggcc gccaacacga aaaaaatgcg cctcggcact tgcgtcacca
     2041 accccgccgt ccgcgatgtc accgtttccg ccagcctgtt cgccaccctg aatctcgctt
     2101 cccatggccg catggaactc ggcattggcc ggggcgacag ctcccgccgc gtcctcggca
     2161 aaaaaccctc cacccttgaa aacctcgagg aatttgtccg catcttccgc aacttgaacg
     2221 ccggcaatac cgtcgacctc gaaggcgtcc ccacaaaatt ttcctggacc aacggcgaaa
     2281 ttccccgcgt ctgggtcgcc ggctacggcc cgaaagccct gcgcaccgcc ggtcgcatcg
     2341 gcgacggcgt gattctccag ttcgccgacc ccgacctgat cgactggtgc ctcggcttcg
     2401 tccgcgaagg cgcgaaggag gctggccgcg acttcagcaa gatcgaagtc atggccgccg
     2461 cgccggtctg ggcctccgac gatctgaaag ccgcgcgcga acacgtccgc tggtttcccg
     2521 ccctcgtctc caatcacgtg atggacctca tctccaaata caagcccgaa gaattgcctc
     2581 ccgcgctgac ctctttcgta aaagaccgcg gcaagtacga ttatctccac cactgcgaag
     2641 tcggcagcga caacgccgag ttcgtcaccg acgaagtcgt cgaccgcttc tgcatcatcg
     2701 gccccatcga cgagcatcgc aagaaactag accgcctccg caaagtaggc gtcacccaat
     2761 tcaatatcta cctcatgtgc ggcgacgaag aaaaaaccct cgacggctac cagcacaaaa
     2821 ttttgcccca cttcaaacat tgacatggcc gatgtccatc acaggcgcgc tatcatgcac
     2881 ggcaccaacc caaaccgcga tgccatgccg ggcctcgacc caaaccgtgc tgtcattctg
     2941 agtgcatccg ataaggatgc acgaagaacc tcaactgaaa gtatctgcaa tcacgtgaga
     3001 ttgagcggac acctgtccac cagcggctcg atgcaggttc ccgcccgtcc acaaaattcc
     3061 ccttgccaaa cccatactcc tctggtacac tatcttccag gtacaattct ggccgcatcc
     3121 gtggcctccc gcggtcccca ttccgacctc aaaaaatgct cgaaaattgc acaatttttg
     3181 tgcaatgtaa gtcctttaga agcaacactt atggatctcc tcgcaagtgt tgcaaacaaa
     3241 agacttacgg catggctaaa ccctttagat gcaacactta caaaaaacat agggggtgcg
     3301 ggtacctcat ttggcccctt tgcatctact tcccagtcac tcaaaatcgg ttcctaagga
     3361 gcgcagatgc gttttgacac cgtaatccgc agcggcaccg tcgtaaccgc caccgacact
     3421 tgcgtcgccg acgtcggcat caacggcgac aaagtcaccg ccatcgccgc gcagcttccc
     3481 accgaaaacg ccacgcggat caccgacgcg accggcatgc tcgtcattcc cggcggcatc
     3541 gatgtccaca cgcacctcga catgcccttc ggcggcacca ccagcgccga tgatttccag
     3601 accggcacga tcgccgccgc ctttggcggt accaccacgc tcatcgactt cgccatccag
     3661 tacaaaggcc aaaccctccg ccaggccttc gacacctgga tgaaaaaagc ccacgacaaa
     3721 gccgtcaccg actacgcctt ccactgcatc atcactgacc tcggcagcgc gcagctcgag
     3781 gaaatgggcc agctcgtccg cgaaggcgtc accagcttca agcttttcat ggcctatccg
     3841 ggcatcttca tgctcgatga cgccaccatc ttcaaggcca tgcgccaggc agcgaaacac
     3901 agcgccctca tctgcatgca cgccgaaaat ggcggcgcca tcgacgtcat cgtgcagcag
     3961 gccctcgccg aaggaaaacg cgcgcccaaa tatcacgcgc tcacgcgtcc caccaccgcc
     4021 gaagccgaag ccacttcccg cgccatcgcc ctcgccgaaa tggccggcgc gcccgtgtac
     4081 atcgtccatc tctcctgcaa cgaagccctc gaaaaagtcc gcgaagcgcg cgaccgcggc
     4141 ctccctgcct acgccgagac ctgcccgcaa tacttgtatc tctcattgga aaatatggac
     4201 gcgcccggat ttgaaggcgc gaagtacgtt ttcactccgc cgctccgcga aaagtggcac
     4261 caggaaaagc tttggcaagg cctcgccaaa gacacgctcc aggtcgtctc caccgatcac
     4321 tgccccttct gcttcaagga acaaaaagaa ctcggcaagg acgacttcac gaaaattccc
     4381 aatggcggcc ccggcatcga acaccgccta agccttgttt ataccggcgg agtccacggc
     4441 aaacatttct cgccgaatcg ctttgtccag cttgtcgcga ccgctcccgc aaaactcttt
     4501 ggtctatacc cgcgcaaagg caccattgcc gtcggctccg acgccgactt ggtgatcttc
     4561 gatcccaatc acgaagaagt tatcagcgcc aaaacccacc acatgcgcgt ggactattcc
     4621 atgttcgaag gcatcaagac caaaggcgca cccaaaaccg ttttctcccg cggccgtgcc
     4681 gtcatcgacg ccggcaaatt cgtcggccgc cccggcgccg gccaattcct ccgccgccaa
     4741 acgtacgccg gaatttagta ggcagccggt acagatgatg agctcaatta ccctctttgc
     4801 tgtcggggga gcgatttctt ctcggtgatg gagattaggc actcattctt tgtcgggggt
     4861 ccgagttgtg gtttcgcggg ataaggctct aattccaaga gccaattctt gaatctctgg
     4921 acgttctcga tcgtaagagg gtaattgact tccgggcagg accaatcatg gatgacgtgg
     4981 ccgcacaaat cttcagcatc ccaagcaaac ttaccggacg ccactagcgc aaatccgatt
     5041 gtcgaaagct cttgtggagg taattctgcg gagagtaccg cttcacatag ggatacaagc
     5101 atctcgcgac taatccaaaa ctcctcatcc atgtcttcaa tctcgaccaa gaacttgatc
     5161 ggctccactc tcttggtcga gccgaggata tctctagcaa gcgctgtcgc tgtgacgcgg
     5221 cccaacaaaa aatcgcgcaa gactgtttcg cgcatagggg aacactttcg cacaaatcag
     5281 aattcaagca aagcctgcat tcagtagtgg tgtcatctct aaggggtgta gacacgattc
     5341 caaccgacgg acctagcaga aattgacaca ctacaggctc gcttgacccg tttctttccc
     5401 tcctgctatt gtgagaagtt atcttgggct cagtgtacct actgagcgcc agggctcctg
     5461 tggagggagc gttttcgtct catgggcctg accaaactgg taatccgggg cgcgcgccag
     5521 cacaacctca agaacatcag cgtcgaaatc ccccggaact cccttacggt catcaccggc
     5581 ctttccggct ccggcaaatc ctcgctcgcc ttcgacacca tctacgccga aggccagcgc
     5641 cgctacgtgg aatcgctttc cgcctacgcg cgccagtttc tcgatcagat ggagcgcccc
     5701 gaagtcgacg tgatcgaagg cctttctccc tcgatcgcca tagagcagaa aaccaccacg
     5761 cgctcgccgc gctccaccgt cggcaccatc acggaaattt acgattacct ccgcgttatc
     5821 tacgccaccg ttggcgtgcc tcattgtcca aactgcggca agtccatcac gcgccagtcc
     5881 agcgagcaga tcgtccagtc catcctgcac ggtgaaatct gcaagccgga tgaccgcatc
     5941 atgatcctcg cgcccatcgt ccgcggacgg aagggcgcct accgcaagga actcgaaaaa
     6001 ttcgcgcaag acggctacgt ccgttttcgt ctcaacggcg agcttttccc actcgacgat
     6061 ctcccggtca tcgacaagcg caagaatcac accatcgaaa tcgtcatcga ccgcctgctc
     6121 gtcaagcaag gcatcgccgc gcgcctcgaa cagtccatcg ccaccgcgct caaattggcg
     6181 agcggcctcg tcaccgtcgc catcgtcggc ggcaaagagc ataccttttc ggagaaactc
     6241 gcctgcccgg attgcggcat ctccgttccc caacttgagc cgcgctcctt cagtttcaac
     6301 tccccatacg gcgcgtgccc cgcctgcaac ggcctcggct cgaaatacga tttcgatccg
     6361 tccaagatcg tcaccgattg gacccgcccg ctattcgatg gcggcctcgg cccaggctcc
     6421 ggctcagcga tcctgaagcg cacgctggaa cttggcgcct acgctcacgg cttcgatctc
     6481 gaaactcctt tcgagaagtt tcccgcgcgc gtccagaaca tgctcttgta cggctacccg
     6541 ccgtcgaacg gtagcgggtc aggcgaaaat acttcaggag aaaaaaagcc caaaggcgaa
     6601 aaaagtttcc gtttcccagg catcctaaaa tttctcgaac ggaatttcga agagtccaac
     6661 tccgattcct accgtgagtg gataacgcag tacatgtccg ccacgctctg cggcacgtgc
     6721 catggcaggc gcctgcgccc cgaatcgctc gcggtcaagc tcgctagctg gtccctctcc
     6781 gatttcaccg cgctctcgct cagcgccgcg cgccccgccg ttgccaaaat cctcacgcac
     6841 cttaccgagc gccaaaaaga aatcgctgct cgcccgctgg aagaagtttc cgaacgcatc
     6901 gatttcctgc tcgccgtcgg ccttggctat ctcagcctgg accgctccgc cgctactctc
     6961 tccggcggcg aagcccagcg catccgcctc gccacgcaaa tcggctcgcg ccttcgcggg
     7021 gttctctacg ttctcgatga acccagcatc ggccttcacg cccgcgacaa cgctcgcctt
     7081 ctcggctctc tcgaacagct tcgcaatctc ggcaataccg tcctcgtcgt cgagcacgac
     7141 gaagacacca ttcgccgcgc cgatttcgtc gtcgacctcg gccccggcgc cggcaacgcc
     7201 ggcggctacc tcgtggccca aggcaagcca gcgcaaatcg aagccactcc ggaatcgctc
     7261 accggtcaat acctcagcgg tgccgcgaaa atcgccgtcc cggaaaaacg ccgcagcccc
     7321 aacggcaaag ccattcagat tcttggcgcc agtgcgaaca atctgaagga cgtggacatt
     7381 acgctgcctc ttggcttgct caccgtagtc accggcgtct ccggctccgg caaatccaca
     7441 ctcgtcaacg acattctcta tcgcgctctc gcgcagaaac tctatcgctc ttccgaagcg
     7501 cccgccgcgc atcgcgaaat tctcggtgcc gaccaaatcg ataaagtcat cgagatcgat
     7561 caagccccca tcggccgcac cccgcgctcg aatcccgcca cctacaccgg cgtcttcgct
     7621 cccatccgcg atctcttcgc catgctcccg gaatcgcgcg agcgcggcta ccgccccggc
     7681 cgcttcagct tcaacgtgaa gggcggccgc tgcgaagcct gccagggcga cggcctccgc
     7741 cgcatcgaaa tgaattttct tccggacgtt tacgtcatgt gcgaggtgtg ccgcggccgc
     7801 cgctacaatt cagaaaccct cgccgtccgc tacaaaggcc actccattag cgacgtcctc
     7861 aaccttccgt catccgacgc gctgaaggtc ctcgaaaata ttccgcagat tcagcagaag
     7921 ctcgcaacgc tcgtcgaagt cggactcggc tacgtcacgc ttggccagtc cgccactact
     7981 ctttccggcg gcgaagccca gcgcatcaaa ttggcccgtg agctgtcgaa acgacaaacc
     8041 ggtcgcacgc tctacatcct cgacgaacca acaaccggct tgcacttcga cgacgtccga
     8101 aagctcctcg acgttcttca gcgcctcgtc gatcttggta acaccgtgct catcatcgag
     8161 caccacctcg acgtcatcaa gcaagctgac tggatcatcg acctcggccc cgaaggcggc
     8221 gaaggcggcg gccgcatcgt ggcccagggc ccgcccgagc tcgttgcccg cactaaaaaa
     8281 tcctacaccg gccaggtcct cgcccgcgtc ctcggcatct ccaacaacgg tcaccacaat
     8341 ggcaatgcga agtagtcaca tcgccgcaat ctcgtccctt gtaggggcgc agcaccgctg
     8401 cgccccatct cggcaatctc actcgtgctt ggtgttgtgt ccgttgcgcg ttagagtatc
     8461 tccgtctcaa tgagcatcta ctctatccaa ccgatcacgc tcggcgccgt ccatacctac
     8521 ccgctcggat cgcgcaagag caaagtcagc gtccgcgact ttgcaaagcc ccccaacgcg
     8581 aagacctcgc tcacaaaatt tctcgattca cttcccaaca tccttgcggc cgaagatctc
     8641 cgccatctcc tcgccgcgat ccacacggcc cgcagacagc gcagagccat cctctggggt
     8701 atcggtggcc acgttatcaa agttggcctc gggcccgtct tgatcgattt aatgaaacgc
     8761 ggcttcgttt ccggaatcgc catgaacggc gccgcgctca tccacgattt cgaaatcgcc
     8821 cttgccggca acacctccga agacgtcgaa gccggcatcg gcgaaggccg attcggcatg
     8881 gccgccgaaa ccggccaata catcaacgaa ttcgccaaac tttctcaccg cgtccgcatc
     8941 ggctatggcg aagcggccgg gcaatttctc tccagcggca tcatcgaagt caaacacgcg
     9001 gattccagcg tcctcgttgc tgcttacaaa caccgcatcc ccgtgacgat ccatctcgcc
     9061 atcggcacgg acatcccgca catgcatccc gccgccaacg gcgcggcgct cggcgatgcc
     9121 acccatcacg atttccgctt gttctgcgcg cttgtgcaac agatgcatcc cggcggcgtc
     9181 tatctcaact ggggttccgc cgtccttctg cccgaagttt ttctcaaagc ggtttccgtc
     9241 gtgcgtaatc tcggcgtccc gctccgcccc atcaccaccg cgaacttcga ctttatccag
     9301 cactaccgtc cgttgcagaa tgtagtgaag cgtcccaccg cgtcggctcc cagccgcagc
     9361 ggacctgaat cgcacggcta cgccatcacc ggccaccacg agctgctctt gccgctcgtc
     9421 gccgccgcgc tggccgccga ctggcccaag aaacgccgag ctagaaaacg atgagcctct
     9481 tcgacgacaa cccgccgaac cacacggaag aaaatcttcc tgcaatgtct tcgcctgacc
     9541 agccggagca atcggcatct gcgaaaccat ttgctcagcc gttcgggggc gacccgctct
     9601 cctacgtggc agtcactccc gcggcctcca tcccatcgcg taatttgccc gaagatttgc
     9661 gcatctcctg gtcctggcca catcttgttg tcttcctcat attcgggttc ggcagtctgc
     9721 tcctcgttca aatcggcgcc gtcctgctgc tctccgccaa caaacatctc tcgcagaagc
     9781 aactgcagca gatgatcgaa tccaagccgc aattcctggt cggcaccaac gttctctggt
     9841 acgccttgct ttttcttttt ctctacatga ctctcgcggt cttgcgcggc ttgccttttt
     9901 ggcgctctct cggctggaaa aaactccgcg ccgatcccgc cataggcaaa ggcggaccgt
     9961 ggatgtattt cttctccggc tgcggcctcg ccattttcgt ggccatcgcc agctcgcgcg
    10021 tcaaaaacgc agatcacgtt cccattcaag agcttttcaa gagccgcagc ggcgccatgt
    10081 tgctcatggc catggctgtc ttcgtcgctc cgctggtcga agaaactgtt ttccgcggtt
    10141 atctctaccc tctgttcgcc agtaaattgt ccggccttgc ccaaaacgcc ggcgtcaatc
    10201 cccttcgcgc catccggttc ggcaccttcg ccggaatctt catcaccgga atcttgttcg
    10261 gcctcatgca cggcgcacag ctaggttgga cctggggcct cgtcagcctg cttactctcg
    10321 tcggcgttct ctttaccttt gcccgcgcct ggaccggcac tgtgctcgcg agtttccttc
    10381 ttcacctcgg ctacaattcc atgatcgcca tcacttcgat catcgccacc aagggcttta
    10441 cccatatgcc gccgcatccc tgagccgaag atgtctctat ttatttgcac gattgcgcga
    10501 tgtagtttca cacttgctgt cttactcctg ggtgtgactg gagcaatggc ccaagttctt
    10561 gccacgtgcg gcaaatttgt agagcctctc aatagtgggg ataaaccagc tcctctaatt
    10621 ttcggagagc catatagcgt tccgagaatg cgaatccgat tcatcaatgg tgcgaccggg
    10681 aagttattcc agacagaaag tatctccgtc aattatgggt ggcaatggct ggagtaccct
    10741 taccccgaac atgcttgggg tgcttggtca gatggcgcgg attctctttc ctgcaaacag
    10801 gatcacgagg gctggattga tgcacctgcc cacgaggttc ggccgcgcgg atggtatgcc
    10861 ggctggtaca ctcgttggcc gtggtcccac cggccatatt ttgatcatgt tgaagttttg
    10921 gctttgaccg gttacttcgc aagaacactt ttgcgaccac aagatctccg gagatttgac
    10981 caatatgacc tggttatctc ggtatttgac ggatggcgcg ccgaaattcg ctggcaggca
    11041 agaaagcctt agcgacagtg aaacggtttt ccatcccact cataagaagt ctccgctaga
    11101 attgaattgt gcgaggatga ctccggaatg tacgtagccc aacccatcga atggactggc
    11161 cagggcgtgg tcatgctcga ccagcgccgc cttcccgtcg aggaaatctc ctacacctat
    11221 accgactacc gcgaagtcgc caaagccata cgcgaaatgg tgatccgtgg cgctccggcc
    11281 atcggcgttg ccgccgccat gggcgccgcc ctcggagttc tgcattcttc cgcgaaatcc
    11341 gtcgaagcgc tccgcgcgga atttacggaa atctgcgacg cgctcgcaaa aactcgcccg
    11401 actgcggtgg atcttttctg ggcgctagag cgcatgaagc gtcgcttcgc tgaactgaca
    11461 gccgaaaact cagacttggg caggatcaaa cagaaaatgg ttgaggaagc gcagcagatt
    11521 catcttgaga agcgcgccac cgacgaagcc atcggccgct tcggcgcgga attcatgccc
    11581 cgcgaaggcc aggtcatgac gcaatgcaac gccggggcgc tcgccaccgg cggtatcggc
    11641 accgcgctcg gcgtgattcg cgtcgcgttc gaacagggga aaaagctcca cgtcctggtc
    11701 ccagaaacgc gcccctatct tcaaggcgcg cgccttaccg cctgggaact gcacaagggc
    11761 ggcattccgc tcacgctcat caccgacaat atggtgggtc acttcctgaa gaccgggaaa
    11821 gttggcgcca tcgtcactgg cgcggatcgc atcgccgcga acggcgacac cgccaacaaa
    11881 atcggcacgt accagatcgc cgtcctcgcg aaagaaaaca acgtgccctt ctacatcgcc
    11941 gccccggttt ctactttcga tctttccatc cccaacggcg aacacatccc catcgaagag
    12001 cgctccgccg cagaagtcac tcattttcaa ggcatccgca tcgcccccga cgtccacgcg
    12061 gcgcatcccg ccttcgatgt gactcccgcg cgctacatcg ccgccatctt caccgaacgc
    12121 ggcgtcgccc gcgcccctta caccgatgct ctccgcgccc ttgcctccct ttctcagagc
    12181 gcttccatag cccgttaatc gccctaaaat ctcgcggcca ccctcagccg ctgtcgcggt
    12241 caaaatcaac tcgcgtataa tctttctcga tgctctcttg gtctcgtccg ctcgctcgtg
    12301 ccttcatcct tgtcctcgca tgcattctag ttccctctct gcactcttct ccgcagcctt
    12361 cctggagtgg cgtcctgcgc gactcggcgg gaaatcccgt cgaaatggcc accatcaagc
    12421 tcttcgcggc agagagcaac cgcgagtttt ccgccacaac ttatgcaacc ggccagtttg
    12481 cattcaccgc agtcgcaccg ggcagctaca ggctcacggt aagcgccgcg ggcaagattt
    12541 ggactgcagc ggatcctgtg gtcatcaaag acggcgctac gctcacgtcc ggtcttcaac
    12601 tctcgctaca agggcaggaa cctcggctgg agcttcgcat catggtcgtt caggatgcag
    12661 cttctcccca ggccagcggc ggcgagcatc tttcaactgg agaagtgtcc agcctgccgc
    12721 tgaatgaacg cgacttcagc aagctgctcc tcctcgccgc cggcaccatg acggacagca
    12781 acggcgccgc aaatttcacg cagcaatttg ccgtgaacgg ccagcgcggt tccgccgctg
    12841 tcttcgctat cgatggagcg gacaccaccg accccgaact cggcggcgct accttttcca
    12901 acttcaacgt cgacgccatc caggaagtgc aatccatctc cggggtcatg cctgcggaca
    12961 cgggtcacgg cgcggcagga ttcacgaatg tggtcacgaa gtccggcagc aatcaggtcc
    13021 atggcagcct cttcgaattc gttcgcaacg cctccttcga cgcgcgaaat ttctttgatc
    13081 acaagagcga tgtggacacc cggcatctcc cgccgttcgc ccgcaatgaa ttcggggtaa
    13141 ccaatggcgg cccggtcttt gttccgcgaa tctacgatgg acgcggcaag acattttatt
    13201 tcgcggagta tcagggtttc cggcaagttc tcggaactac tcaggtaatc cccgtgccga
    13261 cgctggccga gaaacaggga atcgacaccg ccacttttcc cggcgatacg ctgattgttc
    13321 cagtcaacag cagcatcgca cccttgctcg cccgctatcc tttgccaaac gatcctcagg
    13381 gcgcttacgg cgcgcgcacc tatgcgactt cttccaaagt tgtcacccgg acggatcagg
    13441 cttcgttccg ggtcgaccat cgcctatccg agaagtccac gttgttcttg cgcgtcagcc
    13501 gcaatcaggt taccgggcca acgaccaacc ccgatcagac cgtgaccgat cctgattttg
    13561 gcgtgaaatt tctcgatcac cagcgcaatg ctgcaataaa atattcgcgc actcccactc
    13621 cacatttcag ctatcaaacg actctcggct tcatccgcag cacgcctctt ttcatcacca
    13681 gcaaccacac ccagccggcg attgccttcg gcgatggtct ctttcaaggc ttcaactccg
    13741 ccgacggatc catcttcggt tcttatggaa acgtctacca gttcaaacac gacatggtct
    13801 acacgcgcgg ctcccattcc ttcaaatggg gcgcggaact gcggttcaat cgcgattcaa
    13861 cgatttttgg attgaatccc aacggactct acactttcgg aggaggcact gcttattctc
    13921 ccgtgttgat tccctccgct agcggccagc acgatattca cccgggagat ccgctcccgg
    13981 attccctcac agggctactc accgcaacac cctattccta caccgtgacc gcggccgcca
    14041 gcctgacccc aaaaggagat aagtttgacg aagccgcggt ccgccgcgag gcctacaatt
    14101 tctacttcct cgacacctgg aaagccaccg ctcgattgag cgtcaactat ggcttgcgct
    14161 atgaagtgaa cagccgcatc catgaagcga aacatcgcac gtcgctggca attccgattg
    14221 ggccaaacgg gcagcccgcg gatttcctca ctcctggcac aaaacaaatc tacgtataca
    14281 atccgcaacc tccctacgct atggattgga acgggtgggg cccgcgccta tctgtggact
    14341 acggcttgac caagcacact acgctccacg ctggcggcgc catcaccacg atactgccta
    14401 acctgtggct cgagaattat gtcaccgggg catttccctt catctttcaa ccgttggtga
    14461 ccgctctgcc cggctttccg ctgagctttc aggatgctgt tgtgcccctc acgcttcctc
    14521 ctgtttacac cacgcagggg aagctcctct tttcctccgg aagcagcagc aacgttgctc
    14581 ccaacacacc gatcgatgtg cagaaattcc agaacgattt ggccgctctc acccccggca
    14641 atcaggttca attattctcg gccatcagct ttgatccaca ttcccgcaac ggttacatcg
    14701 ccacggaaac tttgggcatc gatcatgaaa tcgggagcgt gaaattgagt gcttcgtatg
    14761 tgggcaccgt tggagttcat cttgccagcg tcctctcgcc gaacggctat ggaggtgcgg
    14821 acccggcctt tgcgccctac acacaattca actcggcggg ccatgctatc ggcggtttcg
    14881 gtcccgagtc cgtgatgact accggatcgc actccagtta tcacgcgctg caaacgagcg
    14941 tcagccagaa caccgcccgg atgggcctca gcttccaggc gagctacacg ttttccaaaa
    15001 gtattgatga caccagcgcc gtacctggag gcatttccgg aagtcccggc gtgactttgc
    15061 aggccttgcc gcaggatccc tttaatccta aagccgacaa gggcccttcc actttcgatg
    15121 tgacccatgt tttcacgcta agcctgatgg aggccctgcc cttggatcgc gtgcgctttc
    15181 tccagccgtt gggcaagacg cttaccaaag gctggcagct cctgaacatc accaccttga
    15241 cgaccggtcc cccgttcacc gtttattccg gtgtccagca gaccggcgca ggcgctggag
    15301 gaaccgatcg ccccgacctt ctggccatgc cgaatttttc caccagccgc accgtgcgcg
    15361 aggactactt cggcatgggc gcggccaacg acaccttctt tcaaatcccc atcaacatcc
    15421 ccggcgggac cggaccaaac cagggccgtt tcggcacgct tggccgcagc acatttcgcg
    15481 gccccggctt tcgcgatttt gacatggcct tgatcaagaa cacctctttc ggccatcgcg
    15541 gaaatggcga actcgctatc cttcaatttc gggccgagtt cttcaatatc ttcaacgaag
    15601 tcaatttcgg cttgccgtcc aacatcgtcc tcggcagcgg cttcggcgtc atcagcaaga
    15661 ccgcgggtcc atcgcggcag atccagtttt ccctcaaact gaactattga cccttcgtcc
    15721 tcgcttcatt tcccaccggc cgaatcacaa agcctgttcg cccgcggcgt cgaaacgacg
    15781 cgaaaaaatt cagcaactgc ttccacggcg catatttctt gttcagcaga cgatttccat
    15841 attcggcgtc ggctcccgtc acgatttccg cacgcgcatc cacccactct cccaacaagt
    15901 tgccacgcat atcgcacggc gcgatgcgca cgcgtccatt gttccgaatg cgcttcacct
    15961 ttccggagtt tccaatggtg tacgcgtaca atcgcgccgc atcgctcgca agatcgctag
    16021 ccggatccgc cgcgaaccat acgggcgtct tcacgccatc gccacttttc ttgaatgtct
    16081 cgagattcaa gtacttgtgc ccagcaaacg ccgcaaagcc catcgcatcg ctcctgctac
    16141 cgcgtcagtt cgcaacagtc acttcgatgc gcaccatttt acatgcgcca caaacacaaa
    16201 gggcactccg acctggccgg agcgcccttt gcgccacttc ggaaagtttc ttcttacttt
    16261 gtccggacgt acgtcacatc ttccgtaaac gcatccttct gttctttccg gaacgtccag
    16321 gtggttttga aatgatcgtt gtcgataaac gtgtacaccg cgtgatgcat gtgtccgtca
    16381 tcggcgctct ttagatttga gatgctcacc aggtcgaacg taattgtctt cgtctccggc
    16441 gcgtacgatc ccttcatggt cggctggttg ccggccatgc aatggtgtgt cagcttcacc
    16501 tggtcgccat ccaggtaata gagcgtggtc atctccaccg gcttgccgct ctccaccatc
    16561 cggaacttct caaggacggc cgtgccgccg gatgtcagct cgaggtccag cgtcgccttg
    16621 ttcatgttcg tcttgtcggt ctcccagtgc cctaccagcg ccttcaattt ttcaaacgct
    16681 tccgccccgc ttccggccgc tgaattcggc atcggcataa ttcccatgct tagcaccagc
    16741 acacctagaa ttttccacat gcttctcatt tgtcgatctc ctttcagcca atgctcttgt
    16801 tcaagaacaa aaatgaccgc ccgaaaccca tcgcgcaacg cgcagctcgt atttcacttt
    16861 gcattcttgt cggcctgcat cacgccgaag agatttcctt ccgtgtcttc gcaataggca
    16921 aaatacccaa tcccgggaat cggagatttc ggcatcatct gctttccacc ggccttcacg
    16981 gctgcggcaa tggcggcgtc caccgattcc acatcgatcg tgttcgtggt cacggaaacg
    17041 gggcccggtt ttttcaacat tccaccgttg atccccggag ttccctgcgc gcccgtggtg
    17101 accagccaat actccatggg cccggcccac ttgtcgaatt tccagccgaa cactttttga
    17161 tagaaagccg atgcgcgttc gggatcactc gccggtattt caaaatggat gactcgagac
    17221 atagttctct ccctaatttg tacaaacgaa gtttttgtct tttctctcgg tcttgtctag
    17281 cgcgtcgccg gcgccggcga cgcaccgtag cgctgcgtgg cgtaacccac gatcaagccc
    17341 acaaggcttc ccggaataat gatctgggca tagtagtgtt tcccggtgat accgtcgggc
    17401 atcgccgcga tcgcaaaagc cagcagtgca ccgatggcca agccgaacac aatgccgaga
    17461 ggcagagagt tcaccttgcg tgagaaaaac ccgattagca cgccaccgac cagccctttg
    17521 aacgtagacc cgatgatgat catcgccatt tccgggcgta cctcgggtgt gaaccacgcc
    17581 gtccctccgt cgaccgcacc caacaccgtt ccgagcaaca gtcccagcag gactttgttc
    17641 atcttctcct ccacctcgat tgtttctcta ccgttcttta caacacttct cccaattcgt
    17701 ttagcttcat ctgtgcataa acagcaaaat caggacgcgc atcgccaagt acgccgcaat
    17761 caataagata aagaaccggt agcgccggta aaaccgcccg gccttctcaa agacggtctc
    17821 ccggtgggca ccgaccgcga gcccgtccag gtatctcgaa acatccagcg ccatctcctg
    17881 aacgctgggg tagcgctctg ccggagacgc cgccgtcgac tttgcgcaaa tggccgcgag
    17941 cgatttatcg aggcggcggc cagtcggaat gggagctggt tccacgtggc cggtgagcag
    18001 gaattgcagc agtgcaccga gcgaataaat gtcactgcgc gcgtccaaat gttctacgtc
    18061 gccgcgcgct tgttccggtg acatgtatcc cggcgtgccc atcaccgtgc cgtgtccggt
    18121 cacgacagaa atttccctgg catcactggc gaaagcgggt tgctttggct tctcgaaaac
    18181 cgtcgcttcc gagtcggctc cgcggacttc gcgcgaaact tctccgtgca agattttggc
    18241 gaggccccag tccatcacca gtatttcgcc gaacggcccg accatgatat tcgcgggttt
    18301 taaatcgcgg tgcagcacgc cgcgcgcgtg tgcaaacgca acggcgtcac aaatgcgaag
    18361 aaaaatgcgc aatcggttcg gcacggacgc gaccgactca atatatttgt cgaggcgttt
    18421 tccttccaca aatttcatgg tgtagaaaac gcggccatcc gcgagtgttc ccacgtcgtg
    18481 cacgggcacg atgccgggat gctcgagagc cgccaggacg cgcgcctcgc gaatcagccg
    18541 gtttgtgaga tcgccgtccg ttcccgggac atcgagcact ttcagcgcca cgcgccgctg
    18601 gagattctcg tcttccgcgg cgtacaccac tcccataccg ccgcgcgcga tgcgttccag
    18661 cagtcgataa cgcgtgccgg cgagatcggg cgcctccgcg ccatcgcgca gtctttcgag
    18721 cgcgctgtcg gaaagaaatc tcatttcgca tcccagccaa aagcggcgcg cgcttcgcgt
    18781 tgaaattctt cttcgctgcc gcagagctcg cgcagacggt cgaga
//
LOCUS       DASYVO010000435        17043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_66615, whole genome shotgun sequence.
ACCESSION   DASYVO010000435 DASYVO010000000
VERSION     DASYVO010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 17043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17043
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66615"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /locus_tag="VGR03_14980"
     CDS             complement(<1..1574)
                     /locus_tag="VGR03_14980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1995633.1"
                     /translation="MSKTSQSLTAIPLGGLGEFGMNMMALRLGDEIIVIDAGMMFPES
                     ELLGVDLVIPDITYLKQNRANVRAIVLTHGHEDHIGALRYILKDLNVPVYGTRFTLAL
                     VKKRLDEAGLLDSTTLREVIPGRRVEIGPFEVEFIAVTHSTVDCVALAIRTPLGVVIH
                     TGDFKIDQTPVGGAPFDLHAFARYGNEGVLALFSDSTNVERPGFTPSERAIVPRIEEL
                     CRSAPRRVILSCFASSIHRIQQVIDIASRVNRKVAFVGRSMVDNVEIAHSLELLRIPD
                     GMVVRPQDIRGFDAKRIIILASGSQAEPMSSLSRIAVDNHRFVSVDENDSVILSARII
                     PGNEKSIFRMLDHMFRRRALVYYDNSAGTIHVSGHASQEEQKLLLQLVKPKYFIPVHG
                     EYRHLFRHAALAHQLGCVSHEILLVEDGRPIEFTEDGARRLDPVTAGRVCVDSGSLEE
                     IEDVVIRDRKHLSEDGIVVPIIAIDKHTGKMESHPEIVSRGLMSDNGQELIAGARLVI
                     MKTVEESNAEEKSDWGV"
     gene            complement(1676..1978)
                     /locus_tag="VGR03_14985"
     CDS             complement(1676..1978)
                     /locus_tag="VGR03_14985"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1995634.1"
                     /translation="MFAEQVITQRTVRVWFESHSIISWLFLGLVAGWLAGKLARGRGF
                     GCITDIILGLIGSYLGGWIFTKLGILGGGFLYSLAAATLGAVILVAIAHAFSGSDR"
     gene            complement(2057..3130)
                     /locus_tag="VGR03_14990"
     CDS             complement(2057..3130)
                     /locus_tag="VGR03_14990"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEV1995635.1"
                     /translation="MKTPPQSKTPSAFGFRMPAEWEPHEATWLGWPHELTDWPGKFAP
                     IPWAFAEIVRHLSKVERVFLLVEDRQSESRVRTILKKSGVNLDAVDFFRVPTDRGWMR
                     DSGPICVRNESGEVAYNHFVFNGWAKYSNHKKDAAVVTKANQKWKRRVWQPEHKGRRV
                     VLEGGSIDVNGRGTLLTTEECLLSKVQERNPGFTKEDYAQVFREYFGVTNILWLKNGI
                     AGDDTHGHVDDLTRFVNPSTVVTIVEDDPKDANYAPLQGNLALLQTMKDQDGRPLRVE
                     TLPMPALVFFDGQCLPASYANFYIANKIVIVPTFNDPNDRVALNTLARLFPDREVVGI
                     ACRDLVLGLGTLHCMTQQQPASG"
     gene            complement(3219..4112)
                     /locus_tag="VGR03_14995"
     CDS             complement(3219..4112)
                     /locus_tag="VGR03_14995"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HEV1995636.1"
                     /translation="MADKFTVGLVQMKCTKNAEENLTRAIGKIREAAARGAQIISLHE
                     LFLGEYFCRTEDAGLFNLSESVPGPTTETLAQVAKEKKVALVVSLFERRAPGVYHNSC
                     AVLDADGSLLGKYRKMHIPDDPLYYEKFYFTPGDLGFPNFDTKYGRIGVQICWDQWYP
                     EGSRITALGGAQVIFYPTSIGWHPHEKAQFGAAQLDAWKTIQRGHAIANGVYAAVVNR
                     VGFEGQPEKGDLGLEFWGNSFVADPFGQVISEACSDREEILVVECDPAKSEETRRNWP
                     FLRDRRIDAYQPILNRWLDKE"
     gene            4274..5344
                     /locus_tag="VGR03_15000"
     CDS             4274..5344
                     /locus_tag="VGR03_15000"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEV1995637.1"
                     /translation="MAKQRKKTVTGRVLHDPVKDKLRPMFPLDLSKCRTIDELVRAMG
                     DTAFTGRQIGDAADVFEAMARDKDCFIVMTLSGALTVGKMGLIFCDLIESGIVKAIVS
                     TGALMAHGLVEATGYSHFRYNPKMGDSELFQAGYNRVYDSLEPETNLDHVEEVMDQIL
                     NEWDAEEIACSWKIHRRIGEYLHKTAPGRGILKSAYEHNVPIFVPAFSDSELGIDFAL
                     HKIARQRDKRPLLRFDPFEDFEKFADTMLATKRMGILTVGGGVPRNWSQQFGVYAELL
                     ARRGYKKLPLKRYNYGVRICPEPVHWGGLSGSTYTEAVSWGKFVPPEEGGRFAEVFDD
                     ATVALPLIVGAVLERIGYFKRA"
     gene            5395..6033
                     /locus_tag="VGR03_15005"
     CDS             5395..6033
                     /locus_tag="VGR03_15005"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HEV1995638.1"
                     /translation="MASAKTARTPLVYDIHPGVAMVQKWIAELKQKTGRSLEEWIAFA
                     RKEGPKDDLRRREWLKNKHKLGTNSAWWISERADGKGGEEDSPEAYLKTAVLYVEEQY
                     AGPKEKLRPIYDELLTLGKSIGSDVKACPCKTMVPLYREHVFAQIKPTTNSRIDLGFA
                     LTHHKGKLPKRVIDTGGLSKKDRITHRIEITAAAQIDGEVKKWLKTAYDLDA"
     gene            complement(6132..6689)
                     /locus_tag="VGR03_15010"
     CDS             complement(6132..6689)
                     /locus_tag="VGR03_15010"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HEV1995639.1"
                     /translation="MAFVAKKIFLTKGVGKHRERLSSFELALRNAGIAACNIVRVSSI
                     FPPNCKLISRSEGLKHIRPGQVAFTVISENSTKEAHRLIAASIGLALPSDKSMYGYLS
                     EHHSFGETEEVAGEYAEELAAEMLATTLNVEFDPDLSFDEKKEVYRISNKIVRTMNVT
                     QSAVGDKRGLWTTVLGAAILIGEDD"
     gene            complement(6856..7395)
                     /locus_tag="VGR03_15015"
     CDS             complement(6856..7395)
                     /locus_tag="VGR03_15015"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV1995640.1"
                     /translation="MSTTLSLVSEMERIASALNTEQGDASLVSLSSVAQKIAKNLGVR
                     NDEVAILAVSTRWRHLHFLVPEALKKVGFIPLSSNSALAARTARDSRPDIENNFAAAR
                     HATVFEGVKITGEAAEAIQKMISAPILLDGKVIGVIQVSRKGANPASAGPDFTADDLG
                     KILTLCKPLGKMLHHVAGE"
     gene            7643..8299
                     /locus_tag="VGR03_15020"
     CDS             7643..8299
                     /locus_tag="VGR03_15020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995641.1"
                     /translation="MSSTATRPDTSYLLRKLHSFTGLLPVGAFLAEHFWSNSSALVSA
                     EKYNTTSQDLQNIPFRLIVEWGGIFLPMLFHGGYGIYIWLRGKSNVSAYPWVGNWLYA
                     TQRYTGIIAFAYIGWHLYTERWLMHGMSTYANVAEDMQNPWYLTFFIVGVLASSFHLG
                     VGIWNFLCKWGLAATVKAQQAAGGFGALVGVAFSIVGILIILSFRFDWHPFSAYLPTK
                     "
     gene            8296..10080
                     /gene="sdhA"
                     /locus_tag="VGR03_15025"
     CDS             8296..10080
                     /gene="sdhA"
                     /locus_tag="VGR03_15025"
                     /inference="COORDINATES: protein motif:HMM:NF006392.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEV1995642.1"
                     /translation="MRQPKIIVVGGGLAGLMATIRIAEAGIPVDLFSIVPVKRSHSVC
                     AQGGINAAKNQKGEGDTVDKHFDDTIYGGDFLANQPLVKRMCDAAPGIIDLLDRMGVM
                     FNRTPEGLLDYRRFGGTLYHRTAFAGATTGQQLLYALDEQVRRHESEGKVKKFEGATF
                     LSAVIDDGGVCRGICAMDLRSMEVKTYAADAVIFATGGNGAIFGRSTNSVVCTGSAQA
                     ALFQQGVDYANGEFIQVHPTAIPGEDKLRLMSESARGEGGRVWVPRNPAEKRAPRNIP
                     EAERFYFLEEWYPKYGNLVPRDIATRAIHRIVYQEKLGIDGQPAVYLDVTHLPRETLD
                     RKLEGILEIYEKFCGVNPREEPMKVFPAMHYTMGGIWVNAEDQATNVSGIYAAGECEY
                     QYHGANRLGANSLVSCIFGGGIAGPAAVKYAKNLEKGAESTASAVFDAERKHQEEKNQ
                     KLISQDGSENPIAIWRELGDVMTEHVTVTRINKNLEMADAKIRALQERYKEINLSDRT
                     KWSNQTLNFARELENMIVLARVVTLGALARNESRGAHYKPDFPDRDDANWLKTTRAKW
                     VNNDIQLTYEPVDISLIKPRPRRYDAAK"
     gene            10123..10875
                     /gene="sdhB"
                     /locus_tag="VGR03_15030"
     CDS             10123..10875
                     /gene="sdhB"
                     /locus_tag="VGR03_15030"
                     /inference="COORDINATES: protein motif:HMM:NF006391.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEV1995643.1"
                     /translation="MAETKQVIVRVKRQAGPKEAARWEEFSLQWRTSMNVIICLRDIA
                     ENPVTRGGQKTTPVSYDSSCLEEICGSCAMLINGKARMACSALVDQLEQPIRIEPMSK
                     FPLVRDLAVDRQFMFESLKRVKAWIPIDGTYNLGEGPRVSPEIQEKGYPLSNCITCGN
                     CLEVCPQVNDNNQFVGAAIISQVRLFNMHPTGAMNKSERLSAIMGPGGIQECANAQNC
                     VHACPKGIPLTESIAEVNREVWKQALLGWLVG"
     gene            11151..12458
                     /locus_tag="VGR03_15035"
     CDS             11151..12458
                     /locus_tag="VGR03_15035"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEV1995644.1"
                     /translation="MESSSGSDPSRESSVLPRILHRPAITALILGVVGLSFFLAGIGR
                     PTMMFYDEGLFVPEARTFLLKAPNPNSPILIHFLAKPPLGKMIMAIGMKAAGDNPFGW
                     RVAAALCGALTLVAVYLWTLLLLRDSRLASFAAGLTLFNNFLFVMSRVGMMDAFYMVF
                     LIWSLVAYTASTALDLGVGRRRFLFICSGILIGLAGACKWNAIDTLAVLLLVSFALLW
                     VARRSPADSISSLSRHAHNIQQIGVLTLLLGLVVAPITTYILTYWPLCRFLHRPFGVH
                     ELMAMHRFSWYICTTWVSNPTIASAWYTWPLNTSPQRALSYLVGNPVVTWGGLVALIF
                     CLWRFWKTVALPEGLVLLLFAANFLQWAVTPEKGLFYYYYYPAVMIMGVAIAVALRSL
                     PRSILGVRVSLVVLVAAAVIFLWCYPRMAHLESPWDCALGCWS"
     gene            12564..13784
                     /locus_tag="VGR03_15040"
     CDS             12564..13784
                     /locus_tag="VGR03_15040"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV1995645.1"
                     /translation="MVRAVLLAAVWISATGLVHLAVSADSISEKARKLHFSSIVVDTH
                     DDTTQRLLDGKFDLGVRSATGSIDIPRMREGNLSAIFFSIWIPSKITGPEAVNRAMTQ
                     IDAVREQVRKHPNDLVLATTAAEIREARKQGKIAALIGVEGGHMINSDLGVLRSYAAL
                     GVRYMTLTHSGNDEWADASTDKAVHNGLTEFGKDVVREMNRLGLIVDISHVSDKTFYD
                     VLETSKAPLIASHSSCRAICDAARNMTDQMIKDLAAKGGVIQINYHVGFLSQEFRDAE
                     KAKPELEKEIQAEINKRCGENEACQLIEGDRVVRDYMNAGKLPRVDYTKIIEHIDHAV
                     KLVGAEHVGLGSDFDGAMMPFGMEDATKLPKITEALLQKGYSEGDVKKILGENTLRVM
                     TEVERVSRELNAGK"
     gene            13832..14632
                     /locus_tag="VGR03_15045"
     CDS             13832..14632
                     /locus_tag="VGR03_15045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV1995646.1"
                     /translation="MNRVKWFVAAMVMGTLFFGCKAKEQTAEAPPPEKPKAEAPAAPK
                     AETPAPAAAQPEATASTPAAAPRPGYDRALLRAALLKEKAPETFQVKFTTTRGDFIVT
                     VNRAWAPIGADRFYNLVKHRFFDNASFFRVVPGFVVQFGISAYPPVAAAWEKANIQDE
                     PVTQTNKRGYLTYAKTSMPNTRSTQIFINLKDNAGLDRQGFSPFGYVDAQGMKVVDML
                     YDQYGDSSGPDQDQISKQGKPYLDKGWPKLDSIKTATLTGPALAAAKP"
     gene            14947..16707
                     /locus_tag="VGR03_15050"
     CDS             14947..16707
                     /locus_tag="VGR03_15050"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEV1995647.1"
                     /translation="MSVAALTEVEERHSREAIEAEIVRFEERIQQLQSGAITADQFRP
                     FRLKHGTYGQRQPGFQMLRIKIAAGVLKPAQLRVLAHIADEYSTGRGHLTTRENVQFH
                     FVKLENVGAAMRLLADAGLTTREACGNTVRNVTACPVAGICPGEAFDVTPYALGVSRY
                     LLRHPDFHDLPRKFKIAFSGCENDGDCAVAGIHDVGLIAQVRGSNGTSHRGFKVLVGG
                     GLGSLPTEAAVLTDFLPEEELLPTIEAILRVFSETGNRKNKLMARLKFVLRAKGIEEL
                     RRLVAEKRKVSQAPAETFVVPSPIQPSLVTIAPAPLTSPATDTQNDPEYDRWAEHNLM
                     SQRQAGYGAVWLKLSAGTLHSNQMRGLADVLERNDLTGVRIAVNQDLVIPWVPFDRVR
                     AIYDELGALELATPGARTISDVTGCPGATTCNLGITRSLTLAEVLSRELDGYTDPEIQ
                     KLRIKISGCPNSCGHHHIADIGFYGNVRKVGGQQAPYYQLLLGGKVKADGVRFARQII
                     AVPARPIPAIIRELLAFYQTDRQPAETFSAWVSRTPDKAIKARLVPLAEVTVTTEDLF
                     VDWGDTETYTLKLGRGECVA"
     gene            16704..>17043
                     /locus_tag="VGR03_15055"
     CDS             16704..>17043
                     /locus_tag="VGR03_15055"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV1995648.1"
                     /translation="MMGKVHLVGAGPGDPELLTLKAARLLAGADVVLHDALVSGAVLA
                     MISPTAEIINVGKRAGHKLLTQDEINALLVSYGRTHSTVVRLKGGDPSIFGRAGEEIE
                     ALRAAGIEYEI"
BASE COUNT         3646 a         4730 c         5018 g         3649 t
ORIGIN      
        1 acgccccagt cggatttttc ctcggcgttg gattcctcga cggtcttcat aatgaccagt
       61 cgtgccccgg cgatcagttc ctggccgttg tcgctcatca gtccgcggct cacgatttcc
      121 ggatgcgatt ccatcttgcc ggtgtgcttg tcgatcgcga tgatcggcac aacgatgccg
      181 tcttcggaaa gatgcttgcg gtcgcgaatc acaacgtctt caatctcttc gagcgagccg
      241 gaatccacgc aaacgcggcc ggccgtcacc gggtcgagcc ggcgtgcgcc gtcctccgtg
      301 aattcaatcg gccggccgtc ttcgacgagc agaatctcgt gtgatacgca tcccagttga
      361 tgcgccagcg cggcgtgccg gaagagatgc cggtactcgc cgtgcaccgg gatgaaatat
      421 ttcggcttca cgagttgcag caagagtttt tgttcttcct ggctggcgtg accggagacg
      481 tgaatcgtgc ccgcggaatt gtcgtaatag actagcgcgc ggcgccggaa catatgatcc
      541 agcatccgga aaatggattt ttcatttcct ggaataatgc gcgccgacag aatcacgctg
      601 tcgttttcat ccacggaaac gaatcggtga ttgtccaccg cgatccgcga gagcgacgac
      661 atcggctcgg cctggctgcc gctggcaaga atgatgatgc gcttggcgtc gaatccgcga
      721 atgtcctgcg gccgcaccac catcccgtcc gggatgcgca gcagttcaag cgagtgcgcg
      781 atctccacgt tgtcgaccat gctgcgcccg acgaacgcca ctttgcggtt cacgcgcgaa
      841 gcaatgtcga tgacctgctg aatgcgatga atcgaggaag cgaagcatga caggatcacg
      901 cggcgcggcg ccgagcggca caattcttcg attcgcggaa cgatggcgcg ctccgacggc
      961 gtaaaaccgg gacgttcgac gtttgtcgaa tcgctgaaca gcgccagcac gccctcgttt
     1021 ccatacctgg cgaacgcgtg caaatcgaac ggcgcgccgc ccacgggggt ctgatcgatc
     1081 ttgaaatcgc cagtgtggat gaccacgccg aggggtgtgc gaattgccag cgctacgcaa
     1141 tccaccgtgc tgtgcgtgac ggcgatgaac tcaacttcaa agggaccgat ctccacccgc
     1201 cggccaggta tcacttcgcg cagcgtggtt gaatccagaa gccctgcttc atccagccgc
     1261 tttttcacca gcgccagcgt gaagcgcgtc ccgtacacgg gaacatttaa atctttcaag
     1321 atatagcgca gggcgccgat gtgatcttcg tggccgtgcg tcagcacaat cgcgcgaacg
     1381 ttcgcgcggt tttgtttcag atacgtgatg tcgggaatga ccagatcgac gccgagtaat
     1441 tccgattccg gaaacatcat tcccgcgtcg atgacgatga tctcgtcacc gagccgcagc
     1501 gccatcatgt tcatgccgaa ttcgccgagg ccgcctaggg ggatggctgt gagggattgc
     1561 gaggtcttgc tcaatgaggc gtcaactccg agacgcaaac gagaatcgta tcacaggacg
     1621 cgaggcagcc acgtcccgag aacaatgttg cgagagattg cagaatgcgg acggctcagc
     1681 ggtccgaacc gctaaaagca tgcgcgatgg ccacgagaat gaccgctccc agcgtggcgg
     1741 ctgccagtga gtacaaaaag ccgccgccaa gaattcccag tttggtgaaa atccagccgc
     1801 cgaggtagga cccgattagt cccagaataa tgtctgtgat gcagccgaag ccgcggccgc
     1861 gtgccagttt tcctgcgagc catccggcaa ccaggccaag gaaaagccac gagatgatac
     1921 tgtgagattc aaaccagact ctcactgtcc tttgcgtaat cacctgttcg gcgaacatga
     1981 agcctcctga gggaacagcg attgtatctg aacctgcgcg caaggcaata aatagaaaaa
     2041 acaaggaagg cttgtgctag ccggaagcgg gctgctgctg ggtcatacag tggagcgtgc
     2101 ctaaacccag cacgaggtcg cggcaggcaa ttcccaccac ctcgcgatct gggaacaacc
     2161 tggccaaagt attcagcgcc acccgatcat tcgggtcgtt gaaggtgggc acgatcacga
     2221 ttttgttggc gatgtaaaaa ttcgcataac ttgcgggcag gcattgcccg tcgaaaaaaa
     2281 ctagcgcggg cattggcaaa gtctcgacgc gcagcggtcg cccatcctga tccttcatcg
     2341 tttgcaacaa agcaagattt ccttgcagcg gagcgtagtt cgcgtccttc gggtcatcct
     2401 ccacgatggt cacgaccgtt gagggattca cgaagcgcgt cagatcgtcc acatggccgt
     2461 gggtgtcgtc gccggcgatg ccatttttca gccaaagaat atttgtcacg ccgaaatatt
     2521 cgcggaagac ttgcgcgtaa tcctctttcg tgaaacccgg atttctttcc tgcactttgc
     2581 tgaggagaca ttcttcggtg gtcagcagcg ttccgcgtcc attcacgtca atgctgccgc
     2641 cttcgagaac gacgcgccgg cctttgtgtt ccggttgcca gacacggcgc ttccattttt
     2701 ggtttgcttt ggtgacgacg gcagcgtctt ttttatgatt cgaatatttg gcccagccgt
     2761 tgaagacgaa atggttgtag gcgacttcgc cggattcgtt tcgaacgcag atggggccgg
     2821 agtcgcgcat ccagccgcga tcggttggca cgcggaaaaa atccacggcg tcgagattga
     2881 cgccggactt tttcaggatg gttcgaacgc gcgattcgga ttggcgatcc tcaacgagga
     2941 gaaacactcg ctcgacttta gataaatggc ggacgatctc ggcaaacgcc catgggatgg
     3001 gcgcaaattt tccgggccag tcggtcagct cgtgcggcca gccgagccag gtggcttcgt
     3061 ggggttccca ttccgcgggc atgcggaagc cgaaggcgga cggcgttttc gattgcggcg
     3121 gtgtcttcat tggggaaaag aatttaacac agaggataca gagttcgcag agaacacaga
     3181 gaatacaaaa gaacacaaag aacgccgggg cgtgcgcact actctttgtc cagccagcgg
     3241 ttcaggatgg gttgataggc gtcaatgcgg cgatcgcgca gaaacggcca gttgcggcgg
     3301 gtttcctcgc tcttggcagg atcgcactcg acgacgagga tttcttccct gtcgctgcaa
     3361 gcttcggaaa taacctggcc aaagggatcg gcgacaaagg aattgcccca gaattccaat
     3421 cctaggtcgc ccttttccgg ctggccttcg aatccgacgc gattcaccac ggctgcgtac
     3481 acaccgttgg caatggcatg accgcgctga atcgttttcc aggcgtcgag ttgcgcggcg
     3541 ccgaattgcg ctttttcgtg cggatgccag ccgatactgg tcggataaaa gattacctgt
     3601 gccccgccga gcgcggtgat ccgcgatccc tccggatacc attggtccca gcaaatttgc
     3661 acgccgatgc gcccgtactt ggtgtcgaaa ttcggaaagc caagatcgcc gggcgtgaag
     3721 tagaactttt cgtagtagag aggatcgtcg ggaatgtgca tcttgcggta tttgccgagg
     3781 agcgatccgt cggcatccag gacggcgcag gaattgtgat agacgcccgg ggcgcgccgc
     3841 tcgaaaagcg aaacaaccag cgcgaccttc ttttcttttg cgacttgcgc aagtgtttcc
     3901 gtcgtcgggc caggcaccga ttccgaaaga ttaaaaagcc ccgcatcttc ggtgcggcaa
     3961 aagtattcgc cgagaaacag ttcgtgcagc gagatgattt gcgcgccgcg cgcggcggct
     4021 tcgcggatct tcccgatcgc gcgcgtcaga ttctcttccg cattcttagt gcatttcatc
     4081 tgcacgagac cgacggtaaa tttgtctgcc atgaaattta gtttagcacg agccggagag
     4141 aaaatatttg cgcgcgtttt gtagtatctt ttgcgcggtg tttgtaatat caattagcat
     4201 catgattgcg cgacttgtgc gcgtggaaga actttattcg taattccgaa ttcattttcg
     4261 tcgaggagaa gcgatggcga agcagcgcaa gaaaaccgtg accgggagag tgctccacga
     4321 tccggtgaag gacaaactcc ggccgatgtt tccgctagat ttatccaagt gccgcaccat
     4381 cgacgaactg gtccgcgcca tgggcgatac ggccttcacc ggtcggcaga tcggcgacgc
     4441 cgccgacgtt tttgaggcga tggcgcgcga caaggattgc ttcatcgtca tgaccctttc
     4501 cggcgccctc accgtcggca agatgggact tattttttgc gacctgattg aatcgggcat
     4561 cgttaaagcc atcgtctcga ccggcgcgct catggcgcat ggactcgtgg aagccaccgg
     4621 ctactcgcat tttcgctaca accccaagat gggtgacagt gaacttttcc aagccgggta
     4681 caaccgcgtt tacgactcgc tcgagccgga gacgaatctc gatcatgtcg aagaggtgat
     4741 ggaccagatc ctcaacgaat gggacgcgga ggaaatcgct tgcagttgga agatccaccg
     4801 ccgcatcggc gagtatctgc acaaaactgc gccagggcgg ggaattctga aatccgctta
     4861 cgagcacaac gttcctatct ttgttcccgc gttcagcgat tcagaactgg gcatcgactt
     4921 cgcgctgcac aagattgccc ggcagaggga taaacggcca ctgctgcgct tcgatccgtt
     4981 cgaggatttc gaaaagtttg ccgacaccat gctggccaca aagcgcatgg gaattttgac
     5041 ggtgggtggt ggcgtcccac gcaattggtc gcaacaattc ggagtttacg cggaactgct
     5101 ggcgcggcgc ggctacaaga aacttccgct gaagcgctac aactacggcg tgcgcatttg
     5161 cccggagccg gtccactggg gcggcctgag cggcagcacg tacacggaag ccgtttcgtg
     5221 gggcaagttc gttccgccgg aagaaggcgg acgatttgcg gaagtcttcg acgatgcgac
     5281 ggtggcgctg ccgctgatcg tcggcgcggt gctggagcgt atcgggtatt ttaaacgggc
     5341 gtagaaaagc tgcagcaagg tgcgccgatt gaagatcgcc aagcggaggt tgtcatggca
     5401 agtgcaaaaa cagcaagaac acctttggtg tatgacatac atcccggcgt ggcgatggtg
     5461 cagaagtgga ttgccgagct gaagcagaag accgggcgct cgctggaaga atggatcgcg
     5521 tttgctagga aagaaggacc gaaagatgat ctgaggcggc gcgagtggct caagaacaag
     5581 cacaagctgg gaacgaatag cgcctggtgg atttcggaac gcgcggatgg caagggcggg
     5641 gaagaagatt ctccggaggc gtatctaaag acagcagttc tgtacgtcga ggagcagtat
     5701 gcggggccga aagagaagct gcggccgatt tatgatgagc tgctaacgct tggcaaatcg
     5761 attggcagtg acgtgaaagc ctgcccctgc aagaccatgg tgccgctata tcgcgagcat
     5821 gtattcgcgc aaatcaagcc gaccaccaac tcgcggatcg atctcggctt tgcgttgacc
     5881 caccacaagg gaaaactgcc gaaacgcgtc atcgacacgg gcggactgtc gaagaaagac
     5941 cgcatcacgc atcgcattga aattacggca gcagcgcaga tcgatggtga agtgaagaag
     6001 tggctgaaga cggcgtacga tttggacgcg tgagaacgat tctgcacaaa ggaaagcgga
     6061 aagcacggag ttgctgccgg caccggagag cggcccgcct gggaaggcgg gctctacaag
     6121 ggacttgcgg cctagtcgtc ttcgccgatg aggatggcgg cgccgagcac ggtggtccac
     6181 aggccgcgct tgtcgccaac ggcggattgc gtgacgttca tggtacggac gatcttgttg
     6241 gagatgcggt agacttcctt tttttcgtcg aagctcaggt ccggatcgaa ttccacattc
     6301 aaagttgttg cgagcatttc cgccgcgagc tcttcggcat attcgccggc aacttcttcg
     6361 gtctcgccga aggagtgatg ctcggacaag tatccataca tggatttatc gctgggcagc
     6421 gccaagccga tgctggcagc gatgagccgg tgcgcttcct tggtggaatt ttcgctgatg
     6481 accgtgaagg cgacttgccc gggcctgatg tgcttcaatc cttcgctgcg agaaatcaat
     6541 ttgcagttgg gagggaagat ggaggagacg cggacaatgt tgcatgccgc aatccctgcg
     6601 ttccgcaagg cgagctcgaa gctcgaaagc ctttcacgat gtttaccgac gcctttggtg
     6661 aggaagattt tcttagcaac gaacgccatg gtgccggttt gccgtgcctc cgtgagtagt
     6721 gtgagcgcga aaaattttcc cgcagtggaa aagtaacaag aaggtattgc agtgtcaacg
     6781 catttccggc cctgaacgcg gcaagaacgg ggaaattatg aacgcgagat gacaaatacg
     6841 gcgggcgcag aaggatcatt cgcccgcgac atggtggagc atcttgccaa gaggcttgca
     6901 gagcgtcagg atttttccga gatcgtccgc ggtgaagtcc gggcctgcgc tggccggatt
     6961 tgcccctttg cggctgactt gaatgacgcc aatgaccttg ccatccagca gaatcggcgc
     7021 actgatcatc ttctgaatcg cttctgcagc ttccccggtg atcttcacgc cctcgaagac
     7081 ggtcgcatgc cgcgcggcgg caaaattatt ctcgatgtct ggccggctgt cgcgcgcggt
     7141 gcgtgccgca agggccgaat tgctcgatag tggaataaag ccgaccttct tcaaagcttc
     7201 cggcaccaga aagtgcagat ggcgccagcg ggtggaaacc gccaggatgg ccacttcatc
     7261 attccggacg cccagattct tggcgatttt ctgagccacg gaggaaagag aaaccagcga
     7321 ggcgtcccct tgttccgtgt tgagggcgct ggcgatgcgt tccatctccg agacaagact
     7381 cagtgttgta ctcatggcaa gtggctgaat gttggggctg aggccgggag aggtgtgggc
     7441 ctagcgaatc ccatccgtca aggttctatt ttccggcact tctgccgccg caagaagatt
     7501 tcgaatagtt tgcgcccgca gaaagaacag accaacttgc atccccggaa tcatgtgcct
     7561 atactgattt cgccgcggat tctcaacgct cctcccgagt cattcattgt gatccggatg
     7621 ggacttacgc ggcggagagg gcatgtcttc gacggcaacc cggcctgaca cgagttatct
     7681 cctcagaaaa ctgcattctt ttaccggcct acttcccgtc ggcgcatttt tggcggagca
     7741 tttctggtcc aacagctccg cgcttgtgag tgcggagaaa tacaacacaa cttcgcaaga
     7801 tcttcaaaac attccgttcc gtctgatcgt ggaatgggga ggcatttttc ttcccatgct
     7861 gttccatggc ggatatggca tttacatttg gctgcgcgga aaatcaaacg tttcggcata
     7921 tccctgggta gggaattggc tctacgcgac gcagcgatat acgggcatca tcgcgtttgc
     7981 gtatattggc tggcatcttt acacggagcg ctggctcatg catggcatgt cgacgtatgc
     8041 aaacgttgcc gaggacatgc agaatccgtg gtacctgacg ttttttatcg tcggcgtgct
     8101 ggcttcttcg ttccatctcg gcgtgggcat ctggaatttc ctatgcaagt gggggctggc
     8161 ggccaccgtc aaggcgcagc aagcggcagg gggctttgga gcgctcgtgg gagtggcatt
     8221 cagcatcgtc ggaatcctga tcattcttag tttccgattc gattggcatc cgttctctgc
     8281 ctatctgcca accaaatgag acagccaaag atcatagtcg tgggcggtgg tttggccggc
     8341 ctgatggcaa caatccgcat tgcggaggcg ggcatcccgg ttgatctttt ctcgatcgtg
     8401 ccggtgaagc gctcgcattc tgtgtgcgct cagggcggca tcaacgctgc caagaaccag
     8461 aagggcgagg gcgacacggt cgataaacac ttcgacgaca ccatttatgg tggcgacttt
     8521 ctggccaatc aaccgctggt caaacggatg tgcgacgcgg cgccgggtat catcgatctt
     8581 ctcgatcgca tgggcgttat gttcaaccgc acgccggaag gattgctcga ctaccggcgg
     8641 ttcggcggca cgctttatca ccgtacggcg tttgccggag caacgactgg gcagcaactg
     8701 ctctacgctc tcgacgagca agtgcgccgc cacgaatccg aaggcaaggt caagaagttc
     8761 gaaggcgcga cgtttctctc cgcggtaatc gatgatggcg gggtgtgccg cggcatttgc
     8821 gcgatggacc tgcgctccat ggaagtgaag acctacgcgg cggacgcggt gatttttgcg
     8881 actggtggta acggcgcgat cttcgggcgc tcgacgaatt cggtggtctg cacgggttcg
     8941 gcgcaggccg cgctctttca gcagggcgtg gattatgcga acggcgaatt catccaggtg
     9001 catccgacgg cgatccccgg cgaagacaag ctccgcctga tgagcgaatc ggcgcgcggc
     9061 gaaggcggcc gcgtgtgggt gccgcgcaat cccgcggaaa agcgtgcgcc gagaaatatt
     9121 ccggaggcgg aacgatttta cttcctggaa gagtggtatc cgaagtacgg gaatctggtg
     9181 ccgcgtgata tcgccacgcg cgcgattcac cgcatcgtct atcaggaaaa actgggcatc
     9241 gacgggcagc cggcggtgta cctggatgtg acgcacctcc cgcgggaaac gctggaccgc
     9301 aagctggagg ggattctgga aatctacgaa aagttctgcg gcgtcaatcc tcgcgaagag
     9361 ccgatgaaag tttttcccgc gatgcattac accatgggcg ggatctgggt gaatgccgaa
     9421 gatcaggcta ccaacgtttc cggcatttac gcggcgggcg agtgcgaata ccagtatcac
     9481 ggggccaacc ggcttggagc gaactcgctg gtgtcgtgta tttttggcgg aggcattgcg
     9541 ggtccggcgg cggtgaagta cgcgaagaac ctcgaaaagg gtgcggagtc aacggcgtcc
     9601 gcggtctttg acgccgaaag gaagcaccag gaagaaaaga atcagaagct catcagccag
     9661 gacggttcgg agaacccaat tgcgatctgg cgcgaactgg gagacgtaat gacggagcac
     9721 gtgaccgtca cacgcatcaa taagaacctg gagatggctg acgcgaagat ccgcgcgttg
     9781 caagagcgct acaaggaaat caacctcagc gaccgcacga agtggtcgaa ccagacgctg
     9841 aacttcgcgc gcgaactgga aaacatgatt gtcctagcgc gagttgtcac gctgggcgcg
     9901 ctggcccgca atgagtcccg tggggcgcat tacaagccgg attttccgga ccgcgacgac
     9961 gccaattggc tgaagaccac gcgcgcgaag tgggtgaaca acgacatcca gcttacatac
    10021 gagccggtgg acatttcttt gatcaagcca cgtccgcgcc gttacgacgc ggcgaagtag
    10081 gagcaatagc ttgggcttgt ggcgggtttt ggccgaggga ttatggcaga aacaaaacag
    10141 gtcatcgtca gagtaaaacg gcaagccggg ccgaaggagg ccgcgcgctg ggaggaattt
    10201 tctctccagt ggcggacgtc catgaacgtc atcatttgcc tgcgcgacat cgccgagaat
    10261 cccgtcacgc gcggcggcca gaagaccacg ccggtctcct acgactctag ttgcctcgaa
    10321 gagatctgcg gctcctgcgc gatgctcatc aacggcaagg cgcgcatggc gtgttcggcg
    10381 cttgtcgatc aactcgagca gcccatccga atcgagccga tgtccaagtt tccgctggtg
    10441 cgcgatctcg cggtcgatcg ccagttcatg tttgaaagcc tcaagcgcgt gaaggcctgg
    10501 attcccatcg acggcactta caatcttggc gaaggcccgc gcgtatcgcc ggaaattcag
    10561 gaaaaaggtt atccgctttc gaactgcatt acttgcggga actgcctgga ggtgtgtcca
    10621 caggtgaacg acaataatca gttcgtgggc gcggccatca tcagccaggt gcgccttttc
    10681 aacatgcacc ccacgggtgc gatgaacaag agcgagcggc ttagcgcgat catgggtccc
    10741 ggcggcatcc aggaatgcgc gaatgcgcaa aactgcgtgc acgcttgccc caaggggatt
    10801 ccactgactg agtccatcgc ggaagtaaac cgagaggtgt ggaaacaagc gctactcggc
    10861 tggcttgtag gataacaggc cagcaagcat tctcttacca caatgcaatc caaagggtaa
    10921 agatttagat ggcggttctt ccggtagttg gtgtgacgcc ggaacggcag aaatacgatt
    10981 tcactccgag agatattaag atattgcgca ccgcaactgt ccgctaggaa gccgcatcca
    11041 gctcccgcgg atacaattgg tgcatggaga atccgtttag gaatggccgt tcctcagtcc
    11101 ttgaaccaat atcgcgagat ccgcaaacgg gcgctcttgc gcaaggatcc atggaatcca
    11161 gttcgggcag tgatccatca agagagagtt ctgttctgcc acgcattctt catcgtccgg
    11221 caatcacagc gctgattctc ggcgtggtgg gcttgtcgtt ttttttggcc ggcatcggca
    11281 gacctacaat gatgttctac gatgagggct tatttgttcc cgaagccaga acctttctcc
    11341 taaaggcacc caacccaaac tctccaattc taattcactt cttagcgaag cctcctttag
    11401 ggaagatgat catggcaatt ggcatgaaag ctgccgggga taaccctttc ggctggcgag
    11461 ttgccgctgc gttgtgcggc gcgctgactt tggtggccgt ttatctttgg actcttctgc
    11521 ttttgcgcga ttctcgcctt gcttcctttg ctgccgggct gactcttttc aataattttc
    11581 tcttcgtgat gtcgcgcgtc ggcatgatgg atgcgttcta tatggtcttc ctgatttgga
    11641 gtttagtggc gtacaccgcg tctactgctc tagatctcgg tgtaggtaga cgcaggtttc
    11701 ttttcatttg ctctggcatt ttgatcgggc tggcgggcgc ctgtaagtgg aatgcaattg
    11761 acactcttgc ggtccttctc ttggtgagtt tcgcactact atgggtggca cgccgatcgc
    11821 ctgcggattc catttcgtct ctttcccggc acgcacacaa cattcaacaa atcggcgtgc
    11881 tgactctgct actcggcttg gtcgttgcgc ccatcactac ttacattctc acgtactggc
    11941 ctctgtgccg gttcctccac cggccattcg gtgttcatga attgatggcc atgcatcgct
    12001 tcagctggta catctgcact acttgggtct ccaacccaac cattgcttct gcttggtaca
    12061 catggccgct aaatacgagc ccccagcgcg cgctttccta tttagttggc aatcccgttg
    12121 tcacgtgggg cgggttggta gcactaatct tctgtctgtg gcgcttttgg aaaaccgtcg
    12181 ccttgcccga gggattggtt cttctccttt tcgcggcaaa ctttctacaa tgggccgtga
    12241 ctcccgaaaa aggccttttt tattattact actacccggc tgtgatgatc atgggtgtgg
    12301 ccatcgccgt cgccttacgt agcttgccgc gcagcatctt gggcgttcgc gtcagcctgg
    12361 ttgtgttggt tgccgcggct gtcattttct tgtggtgcta tccccgaatg gcgcatttgg
    12421 agtcgccctg ggactgcgct ctcggatgct ggtcctgaga cagaaggaac agtagcccgg
    12481 tccgcacgat ccaggtctga aatttcgcgc cttttctccg ctcaattccg tttacaatct
    12541 atgtttcgta ctttggaggg aaaatggtcc gtgccgtgct gttagccgct gtttggattt
    12601 ccgctaccgg tcttgtgcat ctcgccgtct ccgccgattc tatctccgaa aaagcgcgca
    12661 agctgcattt ttcttccatt gtcgtggaca cgcacgatga tacgacgcag cgccttctcg
    12721 atggcaagtt cgatctcggc gtgcgcagcg ctaccggtag tatcgacatt ccccgcatgc
    12781 gcgaaggcaa cctcagcgcg atcttcttct ccatctggat tcccagcaag atcaccggcc
    12841 cggaagcggt caatcgtgcc atgactcaaa tcgacgcggt gcgcgaacag gtccgcaagc
    12901 atccgaacga tttggttctc gccaccaccg cggctgaaat tcgcgaggct cgcaagcaag
    12961 ggaagatcgc ggcgctgatt ggcgttgagg gcggccacat gatcaacagc gatctcggcg
    13021 tcctgcgcag ctacgcggcg ctgggcgtgc gatacatgac gctgacgcat agcggcaatg
    13081 acgaatgggc cgatgcgtcc accgacaagg cggtgcacaa cgggctcaca gagtttggca
    13141 aggatgtggt gcgcgaaatg aaccgcctgg gtttgattgt ggacatctcc cacgtcagcg
    13201 acaagacgtt ttacgacgtt ctggaaacga gtaaagcccc gctcatcgct tctcactctt
    13261 cctgccgtgc catctgcgac gcggctcgca atatgacgga ccagatgatc aaggatcttg
    13321 ctgccaaggg cggcgtcatt cagattaact accacgtcgg ttttctgagc caggagtttc
    13381 gcgacgccga aaaagccaag cccgaactgg aaaaggagat tcaagccgag atcaacaagc
    13441 gctgcggtga gaacgaagcc tgccagctga tcgagggcga ccgcgtggtg cgtgactata
    13501 tgaacgcggg caagctgcct cgcgtggact acacgaagat catcgagcac atcgaccacg
    13561 ctgtgaaact cgtcggcgcc gaacacgtcg gcctagggtc cgattttgac ggcgccatga
    13621 tgcctttcgg aatggaagac gctacaaaat tgccgaagat cacggaggcg ctgttgcaaa
    13681 aaggctactc cgagggtgac gtaaagaaaa ttctcggcga aaatactctt cgggtcatga
    13741 cggaagtcga acgggtcagc cgcgagctga atgccgggaa gtaaaagcaa cggagaagat
    13801 tttagaaaga cgtatagggg gatgttagaa aatgaatcgt gtaaagtggt ttgtcgccgc
    13861 catggtaatg ggaacgctgt ttttcggttg taaggcaaaa gagcagactg cggaagcgcc
    13921 gccgccggag aagcccaagg ccgaggctcc ggccgcgcca aaagcggaaa ccccagcgcc
    13981 agcggctgcg caacccgagg caacggcaag cacgcccgca gcagcgccgc gccctggcta
    14041 tgatcgcgca cttcttcgcg ctgcgctcct caaggaaaaa gctcctgaga cattccaggt
    14101 gaagttcacg accacgcgcg gagattttat tgtgaccgtg aaccgtgcct gggctcccat
    14161 cggggccgac cgcttctaca atctggtgaa gcaccgtttc tttgacaacg ccagcttctt
    14221 ccgcgtagtg cccggtttcg tcgtgcagtt cggcatcagc gcctacccgc ccgttgcggc
    14281 cgcgtgggag aaagccaata tccaggacga gcccgtcacg cagaccaaca agcgcggcta
    14341 cctgacgtac gcgaaaacga gcatgccaaa tacgcgctcg acgcaaatct tcatcaattt
    14401 gaaggacaac gccggtctcg accggcaagg cttctcacct ttcggctacg tcgatgctca
    14461 aggaatgaag gtggtggaca tgctctacga tcaatacgga gacagttctg gccccgacca
    14521 ggatcaaatc tccaagcagg gtaagcccta tctcgacaaa ggctggccca agctcgattc
    14581 catcaaaacc gccacgttga ccgggccggc cctggcagcc gcgaagccct aaaattcatg
    14641 gaccgccatt gattactttt ttgaatgcgg tgcatccaat cttttgcagg gccttccctt
    14701 tgacttaggg gtggccggga gcgtattctt cagtttcatg atgacctgtt gccagatgat
    14761 gtgttgtccc tgcacgtgca acgctgcgtg ttgcacgaca cccatctctg gccagaggag
    14821 tttccggact taagctttag ctgaatcata aagttccgaa gcccacatgc cagagcacct
    14881 ggttatgtgg gctttttgtt ttggatcgag gatttttcag accaaagaac ttgggagcca
    14941 agaactatga gtgtggcagc cttaacggaa gtcgaagaac ggcacagtcg cgaagccatc
    15001 gaagcggaga tcgtccgttt tgaagagcgc atccaacagc ttcagagcgg cgccatcacc
    15061 gcggatcaat ttcgcccctt tcgcttgaag cacggcacgt acgggcagcg ccagccgggc
    15121 ttccaaatgt tgcgcatcaa gatcgctgcg ggggtgctca agcccgcgca gcttcgcgtc
    15181 ctggcgcata ttgcagacga atactcgacc ggccgcggcc acctcaccac gcgcgaaaac
    15241 gttcagttcc atttcgtgaa acttgaaaac gtgggcgccg ctatgcgctt gcttgccgat
    15301 gcgggcctga cgacgcgcga ggcttgcggt aacaccgtgc gcaacgttac ggcctgcccg
    15361 gtggccggta tttgccctgg cgaagcgttt gatgtcactc cctacgctct tggcgtctcg
    15421 cgatatctat tgcgccatcc cgattttcac gacttgccgc gcaagttcaa gatcgccttc
    15481 tccggctgcg agaacgatgg cgattgcgcg gtcgccggca tccacgacgt cggcctcatc
    15541 gcgcaagtcc gcggtagcaa cggaacgtcg catcgcggat tcaaagttct cgtcggcggc
    15601 gggctgggca gcttgcccac ggaggccgcg gtcctcactg attttctgcc ggaagaggaa
    15661 ctgcttccca ccatcgaggc cattctgcgt gttttttcgg aaacggggaa ccgcaagaac
    15721 aagctcatgg cacgattgaa attcgtcttg cgggcaaaag gcatagagga gttgcgccgt
    15781 ctggttgcgg agaaacgcaa agtctcgcag gcgcccgcgg aaacttttgt tgtgccttct
    15841 cccatccagc cgtcgctggt cactatcgct ccggcacctt taacttcccc cgcgacggac
    15901 acgcaaaacg atcccgaata cgaccgctgg gccgagcaca atctgatgtc tcagcgccag
    15961 gccggatacg gcgcggtgtg gctcaaactt tccgcgggta cgctgcacag caaccaaatg
    16021 cgcggcctcg cggatgttct ggaaaggaac gatctgaccg gcgtgcgcat tgccgtcaat
    16081 caggatctcg tcattccgtg ggttcctttt gaccgcgtgc gcgcgatcta cgacgagctt
    16141 ggcgcattgg aactggccac gcccggagcg cgcactattt ccgacgtcac gggctgcccg
    16201 ggagccacaa cttgcaatct ggggatcacg cgctctctta cgctggccga ggtgctttcg
    16261 cgcgagttgg acggttacac cgatcctgaa atccaaaagt tgcgcatcaa aatcagcggc
    16321 tgccccaatt cctgcgggca tcatcacatt gccgacatcg gtttttacgg caacgtccgg
    16381 aaggtcggcg gacagcaggc tccttactat cagcttttgc tcggcggcaa agtgaaagcc
    16441 gatggtgtgc gctttgcgcg ccagatcatc gccgtgcctg cgcggcccat ccccgcaatc
    16501 attcgcgaac tcctggcttt ctatcagacg gaccggcaac cggctgaaac gttttccgcg
    16561 tgggtgtcgc gcacgccgga caaggccatt aaggcccggc tcgttccctt ggcggaagtc
    16621 acagtcacaa ccgaggatct cttcgtggac tggggcgata cggaaaccta caccctgaag
    16681 ttgggccgcg gcgagtgcgt ggcatgatgg gcaaggttca tctcgttggc gctggaccgg
    16741 gtgatcccga gctgctcacc ttgaaagcgg cacgcctgct cgcaggtgcc gacgttgtcc
    16801 tgcacgatgc gctcgtcagc ggtgctgtat tggcgatgat ttcgcccact gccgagatca
    16861 taaacgttgg caagcgcgcc ggccacaagc tccttaccca ggatgagatc aacgcgcttc
    16921 tggtttcgta tggccggacg cactcgaccg ttgtccgact gaaaggcggc gacccgtcga
    16981 tctttggccg cgccggggaa gaaatcgaag cccttcgcgc agcgggaatt gaatatgaaa
    17041 ttg
//
LOCUS       DASYVO010000436         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_168782, whole genome shotgun sequence.
ACCESSION   DASYVO010000436 DASYVO010000000
VERSION     DASYVO010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168782"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VGR03_15060"
     CDS             complement(<1..318)
                     /locus_tag="VGR03_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995649.1"
                     /translation="MSWRTVFTAALLCFAAAAYAEVADMSSSGFLTKYEVTVNATPAN
                     AYKALVDEVGGWWNPAHTYSNDARNLFIDARPGGCFCEKLPNGGGVQHMSVVYASPGQ
                     VVRM"
     gene            complement(803..1096)
                     /locus_tag="VGR03_15065"
     CDS             complement(803..1096)
                     /locus_tag="VGR03_15065"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEV1995650.1"
                     /translation="MATYIMLTSFTDQGIRNVKDSTKRADAVRELAKKFGVTAKEFFW
                     TLGSYDVVAIYDAPDDASMTALGLAIGSAGNVRTQTMRAFSREEMNGVLAKLA"
     gene            complement(1184..>2051)
                     /locus_tag="VGR03_15070"
     CDS             complement(1184..>2051)
                     /locus_tag="VGR03_15070"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF561 domain-containing protein"
                     /protein_id="HEV1995651.1"
                     /translation="VNFFCHAATTPSAVREAAWRTALAPYYKEYGIDADSIAVGPGRT
                     PFSSEAADVLSEFKPAIVSFHFGLPSIELLARVRAWGPKILSSATTVEEALWLEARGV
                     DAVIAQGIEAGGHRGIFLSDDLSSQVGTIALLPQIVRAVKLPVIAAGGIADARGVTAA
                     LALGAAGVQIGTAYLLCPEATTSVVHRAAVKSEAARHTALTNLFTGRPARGIVNRIMR
                     ELGPISTAAPAFPLATVAIAPLRAKAESRGSGDFSPLWSGQNASGCKEIPAAQLTREL
                     ATDIARRPPGAG"
BASE COUNT          425 a          634 c          593 g          399 t
ORIGIN      
        1 catccggacg acttgacctg gggacgcata cacaacgctc atgtgttgca cgccgccgcc
       61 attgggcaac ttctcacaga agcaacctcc cggccgcgca tcgataaaca ggtttctcgc
      121 gtcgttcgaa taagtgtggg caggattcca ccatccgccg acttcgtcaa cgagcgcctt
      181 ataggcgttc gcaggcgtgg cattgacggt cacctcatac ttggtgagaa accccgatga
      241 actcatgtcc gcgacctcgg cgtatgccgc ggcggcgaaa cacaggagcg ctgcagtgaa
      301 cacagtgcgc cacgacatac gaggatctcc aagaatggga taacacattg cggcaactga
      361 atcgaatcgg gggagcagca ttttcattct acgaggtggc ctccgaagcc aagcctgccg
      421 cgcgctgctg gcgcgtattc aacgtgcaca caggacacgg ggcggcctgt gatctctgcg
      481 attgcacttg ccagctccat gatttcgccc gcaagttccg ccccttccgg aaccgcccgc
      541 ttaagcaccc taacaaagac tggcaattcg gttgtttcga ggcacgattc attctctgcg
      601 taatgatctc gctcaagtac gtgtaaccga acccaggttt ggccgggcgg cgattggaat
      661 gtacgaccca cggcgtcggc aagcgactga gcgaggcccg ggacgaccac gccactggca
      721 cccgcgacaa gctcaacgtc cactatgggc acggttgatc accaccacgg ccgaatgagg
      781 ggtcaggcag gcgcattttc tcctatgcca gcttggcgag aactccgttc atttcttctc
      841 tggagaaagc tcgcatggtc tgtgtgcgga cgttacccgc tgaaccgatg gccaatccga
      901 gggcggtcat agaggcatcg tcaggtgcat cgtatattgc aacgacatcg tagcttccca
      961 gtgtccagaa aaactccttc gcggtaaccc cgaatttttt ggccagctcc ctcaccgcat
     1021 cggcccgctt tgtgctgtct ttgacgttgc gaatgccttg atccgtgaaa ctggtgagca
     1081 taatgtaggt agccatggcg atttctcctt acgttgttgg aggccggtga agcaactgcc
     1141 gctggcggca cggcctcgaa ttgtgcctgc caaacgaacc aagctagcca gcgccgggcg
     1201 ggcggcgcgc aatgtcggtg gccaattcac gagtgagttg agcggctggg atctccttgc
     1261 atccgctagc attctgcccc gaccacagag gcgagaaatc accggagccc cggctttccg
     1321 ctttggcacg caagggcgcg atggcgacgg tagccagggg aaaagcaggc gcagcggtgc
     1381 tgatcgggcc cagctctctc atgatgcggt tcacgattcc gcgggcgggt cgaccggtga
     1441 aaaggttggt gagcgcagta tggcgggcag cctcactctt caccgcggcg cgatgcacca
     1501 cgctcgtggt cgcctcaggg cagagcagat aagccgttcc aatctgtacg ccggctgcgc
     1561 ccagtgccaa cgcagccgta acgcctctcg cgtccgcgat gccgccagca gcaatgaccg
     1621 ggagcttcac tgcgcgcact atctgcggca acaatgcgat cgtgccaacc tgggagctta
     1681 gatcatccga caggaaaatg ccacgatgtc cacccgcctc gataccttgt gcaatgacgg
     1741 catcgacccc acgcgcctcg agccaaagcg cttcttcgac ggtcgtcgcc gatgagagaa
     1801 tcttcgggcc ccaggccctc acccgcgcca gcaattcgat tgatggcaaa ccaaaatgaa
     1861 agctcacgat ggcgggcttg aattcactta gcacgtcggc cgcttccgaa ctgaagggcg
     1921 tgcgtcccgg cccgacggca atgctgtctg catcgatccc atactctttg tagtagggcg
     1981 caagcgcagt tcgccatgcc gcttcgcgca cggcgcttgg cgtggttgct gcgtggcaaa
     2041 agaaattcac g
//
LOCUS       DASYVO010000437         1951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_218654, whole genome shotgun sequence.
ACCESSION   DASYVO010000437 DASYVO010000000
VERSION     DASYVO010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218654"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VGR03_15075"
     CDS             <1..675
                     /locus_tag="VGR03_15075"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HEV1995652.1"
                     /translation="MICCDKALAKMGIKKEGCRVIVQGFGNVGSLAAQLMQKAGYKIV
                     GLADIGGGLYNEKGFDVTKVIDWVYTQKKALQDFPGGGTKMSARDVLFQPCEIAIPAA
                     IENQITEKNANQVQAKILCEGANGPTTWQADAIIDAKGIFTVPDILGNAGGVTVSYFE
                     WVQDRQGFFWRESEVNERLLDVMENAFDEVVRYAEVHKVNNRIAAYMLAIDRVAKALK
                     LRGIYA"
     gene            828..1637
                     /locus_tag="VGR03_15080"
     CDS             828..1637
                     /locus_tag="VGR03_15080"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEV1995653.1"
                     /translation="MNTDSLLPGHAKSEEQHRREICIAGSWMYERGYIVECEGNLSVR
                     LDDGSVLNTPTCMNKGTLYSEDLVITDLDGRHLSGSRKVSSEVAMHLLFYRMRPDVNA
                     ICHAHPPTATGFAVSGRALNQALLPEVIVGLGQIPLVQYATPGTAELSAAIEPFVPHY
                     DALLLANHGAVTCGPDLLTAFFRMETIEHCAKITLSAELAGEPALLSSREVAKLMAAR
                     PRYFVAPPPGGGAELPITCDSGETAGDHVTLTRSELDALIDEAVRKDRSRR"
     gene            1699..>1951
                     /locus_tag="VGR03_15085"
     CDS             1699..>1951
                     /locus_tag="VGR03_15085"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEV1995654.1"
                     /translation="MSGITLFFQAIQAGDAGKVKALLDSNASLATAKNEKGQSALLMA
                     VYSGRKEIRDLLLARDFELELDEAAAAGQLERVKQIVGKN"
BASE COUNT          421 a          523 c          634 g          373 t
ORIGIN      
        1 atgatctgct gcgacaaagc gctcgcgaaa atggggataa agaaagaggg ctgccgcgtc
       61 atcgttcagg gctttggcaa cgtgggctcg ctggcggcgc aactgatgca gaaagccggc
      121 tacaagattg tgggacttgc ggacatcggc ggggggctct acaacgagaa gggcttcgat
      181 gtcacgaagg tgatcgactg ggtctacacc cagaaaaaag cgctgcagga cttccccggc
      241 ggcgggacga agatgagcgc gcgggatgta ctctttcagc cgtgcgagat tgccatcccc
      301 gcggcgattg aaaatcagat cacggagaaa aatgcgaatc aggtgcaggc gaagatcctg
      361 tgcgagggcg caaacgggcc aacgacctgg caggccgacg cgatcatcga tgcgaaggga
      421 atattcaccg tgccggacat tctcggcaat gcgggtggcg tgaccgtcag ctatttcgaa
      481 tgggtgcagg accgccaggg tttcttctgg cgggagagcg aagtgaatga gcgcttgctc
      541 gacgtgatgg agaatgcgtt cgacgaggtg gtgcgatatg ccgaggtgca caaagtaaac
      601 aaccggatcg cggcgtacat gctggcgatt gaccgcgtgg cgaaggcgct gaagttgcgc
      661 ggaatttacg cctagcatct tgacgcgagt tgtaatagtt tcttttcact cacgctggcc
      721 cgatcgagtt ggcgtgagtc attccgtaat ttccctacgg agtgcgcccg gaacaattca
      781 ggttgaacgg cccggaggaa cagtccgcgc caggaaggag tccgcccgtg aatacggact
      841 cacttttgcc aggccacgcg aagagcgaag aacagcaccg ccgcgagatc tgcattgcgg
      901 gaagctggat gtacgaacgc gggtacatcg ttgagtgcga ggggaatctc tccgtgcgcc
      961 tggacgatgg cagcgttctg aacacaccca cctgcatgaa caaaggaacg ctgtactcgg
     1021 aggatctggt tattaccgat ctggatgggc gccacctcag cgggtcccgg aaggtttctt
     1081 cggaagtggc gatgcatctt ctgttttacc gtatgcggcc ggatgtgaac gcaatttgcc
     1141 acgcgcaccc gccgacggcg acgggatttg cagtgtcggg gcgcgcgctg aatcaggccc
     1201 tgcttccgga agtcatcgtg ggacttgggc agattccgct ggtgcaatat gcgacgccgg
     1261 gcacagcgga gttgagcgcg gcgatcgagc cttttgtgcc gcactacgac gcacttctcc
     1321 tcgcgaacca cggagcggtg acctgcgggc cggacctgct cacggcgttc tttcgaatgg
     1381 agacgattga acactgcgcg aaaattacgc tgtccgcgga gttggcaggc gaaccggcgc
     1441 tgctctccag ccgggaagtg gcgaagctga tggcggcgcg cccgcgctat ttcgttgctc
     1501 ctccgccggg cggaggagcg gagttgccga ttacttgcga cagtggggaa acagcgggcg
     1561 atcacgtgac cttgacgcga agcgagctcg atgcgctgat cgacgaagcc gtgcgcaagg
     1621 accgtagccg gcgataattc tcgtttcctg catcgcaggt cttcgctaac agcgccccaa
     1681 ttcgcccgga gctcttacat gagcggcatc actctattct ttcaagccat ccaggccgga
     1741 gacgccggca aagtgaaggc gctgctcgat tccaacgctt ctcttgcaac cgcaaaaaac
     1801 gaaaagggcc aatccgcttt gcttatggcc gtgtatagtg gtaggaaaga gattcgcgat
     1861 ctgctcctcg cgcgtgactt tgagctggaa ctggatgaag ccgctgcggc ggggcagctt
     1921 gaacgcgtga agcaaatcgt cggcaagaac c
//
LOCUS       DASYVO010000438         7893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_168883, whole genome shotgun sequence.
ACCESSION   DASYVO010000438 DASYVO010000000
VERSION     DASYVO010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7893
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168883"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..1593
                     /locus_tag="VGR03_15090"
     CDS             13..1593
                     /locus_tag="VGR03_15090"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEV1995655.1"
                     /translation="MSETGMAVAAADPLVVDDYLPRIRFQLPSSGQSIEISAQIVWLA
                     KSKKSAGIRFVDLTAEARNQISNWIASEKPAPEFEGLTPAPVESLIPATLESFPEEPI
                     GSVFPEQDQPSSPELIAHTASQASKIVTPEILDANPPSFVEDLQEKVHHHAPVAGFGP
                     QVRTGKVESSADRIENSPSRLYVLEISGFQVAAVVFLFAVISLTVGLTVGRGPLGKRL
                     RDTQKSLPAVDATSPALLNRPGETTSRNSTPPAANTFLTPAVNPPAPATAGSRSESPS
                     AQSLNARPEDSTTRVGPIGPSSAITNRSLTDSDNPEASPERKGSTGLIARNAPPPANS
                     QPAHSPRAVGPISGAPANPAQRRVTPATRAAPHLSSPSTFLVTGPGDGSKPFRLNLPE
                     EPIAASSSFAMTSQLSVLVAPEPGPSATHKPARLQAGELVSFVWPRYPRPGDRYGSSE
                     TVKVRTTIGKLGQVLDIKLVSGSISLLPAAMSAIRLWRYKPTLLNKRPVQVQQDVTIE
                     FQPLQYLSHVPTQHPSHN"
     gene            complement(1985..2428)
                     /locus_tag="VGR03_15095"
     CDS             complement(1985..2428)
                     /locus_tag="VGR03_15095"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV1995656.1"
                     /translation="MIVMLPEGAKSAPDPVLEILKNYKCVAVVGISSKPFRASNEIAV
                     YLKDAGYRMIGVNPNETRVLGEPCYSRLQDIPEPVEIVDIFRKAPDVPPVVDDAIAKG
                     AKVIWMQLGIENRVAAKKALAAGITVVMDACILIEHQKRLRQLSS"
     gene            complement(2425..3555)
                     /gene="hisC"
                     /locus_tag="VGR03_15100"
     CDS             complement(2425..3555)
                     /gene="hisC"
                     /locus_tag="VGR03_15100"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEV1995657.1"
                     /translation="MTHAIEELIPDYIRGLPAYIPGRPVEEVERELKIHAIKLASNEN
                     PLGPSPMAAEAVKRAVEESNRYPDGGSNLLRKALAARHGVTPEEIFVGLGSSEIIDLA
                     ARCLLRPGLAGVTSEGSYAPYSLAIRAAGAELLQAPLRKYTYDLSAIARRVTPQTRIV
                     YLANPNNPTGTAFGGDELDEFLSRTRKDVLIVLDEAYIHYAERPGMPRSEELYRKHGN
                     LLILRTFSKVYGLAGLRIGYGIGPTGLLAAFNRLKTPFNTSGVAQAAALAALEDKEHV
                     RRSVETNRAERRRLIEGLVKLGLQPVPSETNFVFVEMGAQTSRICDELLHRGVIVRPL
                     GWMGFPEAARVSAGRPEENERFLEAISEIWPVFSAKRELAKR"
     gene            complement(3552..4325)
                     /locus_tag="VGR03_15105"
     CDS             complement(3552..4325)
                     /locus_tag="VGR03_15105"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEV1995658.1"
                     /translation="MQERLQKIIARAGIASRRHAEQLLLSGQVRVNGRVVTQLGTKAD
                     PEKDRIEAAGHVIRGEERKVSLLLHKPAQVLSTMADPEGRKTLRNCLRGLSTRVYPVG
                     RLDYDASGLVFLTNDGDLAAEMLREWHRLPQLYYVKVKGRLPETVVDSVAKRAGVKLR
                     LIRQPGATRGHAVNYWYEATLRDSRRDVLRKVLLAEQHPVEKFKRIAIGPITLEGVPQ
                     GQYRILSEAETGPLRRALRRPATKSTRGRKTSEELPPAR"
     gene            complement(4393..5079)
                     /gene="scpB"
                     /locus_tag="VGR03_15110"
     CDS             complement(4393..5079)
                     /gene="scpB"
                     /locus_tag="VGR03_15110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEV1995659.1"
                     /translation="MTNEERRAALEAIIYAADEPATVQQLAQALGEEKLAVQAALDDL
                     VSNCAGDDRGIEVRAVGGGYKIYTKPQHHEVVRRFIKSLRPPLRLTMPALETLAVIAY
                     KQPATAPEINEIRGVNTAGVLKTLLDKRMITTAGRKEVIGRPILYRTSKEFLMRFGLS
                     DLDELPSLKEFEALAREALGDEGIALQEEAAGEAGFSNETAQAPNSGAMPAENAEPGG
                     EGPKAATAGE"
     gene            complement(5231..6010)
                     /locus_tag="VGR03_15115"
     CDS             complement(5231..6010)
                     /locus_tag="VGR03_15115"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV1995660.1"
                     /translation="MATPYKIQIPIYEGPLDLLLDLIRKQEINIHDIPIAKITAQYLD
                     YLHKLEELNIDVSGDFVYMAATLIYIKSKMLLPPDPLGGPEEQLDPRADLVHRLVEHE
                     KFKSAAQLLYQKQQIEEHVWSKPDKTLYEDEATEGEVVVSLMDLIKVFQQVIERRREV
                     SRIDLQHEQFTVAQMMAQLRAQLLTSDGGTISLVAFFEACPSRHAMIVAFLAVLEMVR
                     LQAVAMVQEKPFGEILLRRDRRFDAVFDESGGIRQIDEQYQ"
     gene            complement(6039..7310)
                     /locus_tag="VGR03_15120"
     CDS             complement(6039..7310)
                     /locus_tag="VGR03_15120"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995661.1"
                     /translation="MTAGTTKRVVSGMRPTGRLHIGHYFGALQNWVKLQDDPEYECFY
                     FIADWHALTSDYADTSAISQNVVDIATDFLAAGLDPKKCVIFLQSLVPEHAELHLLLS
                     MVTPLGWLERVPTYKEALENIKDKDLHTYGFLGYPVLQCADIVMYGEEGKSLIVPVGE
                     DQVSHVELSREIVRRFNAGFARVNWTAVTQEQEAALAKHFYPTSGSAAWKDAGGTSPA
                     KMDSAMRGAPLDYRHSIFANENIPTLSVLREPEVMLTRTPRIPGTDGRKMSKSYGNAI
                     SLSESDEAIWEKVRPMMTDPARKRRTDKGNPDICPVYDWHKLFARGEEGRKTLDWAAH
                     GCRTAEIGCIECKRAMADNLIKWIEPVRERRVEYEKHPARVLEILDAGSQKARQVAQA
                     TMEQVREAVIGWERKREEIAAAVAKRAQATK"
     gene            complement(7380..>7893)
                     /locus_tag="VGR03_15125"
     CDS             complement(7380..>7893)
                     /locus_tag="VGR03_15125"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV1995662.1"
                     /translation="NRLGEEGQGLKIALATLDGGRIGIGALAVGLAQGAMEEAVKYAK
                     QRRAFGKTISEFQAIQWMIADMQTEIEAARGLVYHAAWLKDSGGRLGAQASKAKLYAS
                     EMVNRVVYKAVQVHGAAGYSRETDVERMYRDARVVSIYEGTSEIQRMIIAREILEKVA
                     KAQEVTVGAD"
BASE COUNT         1438 a         2712 c         1986 g         1757 t
ORIGIN      
        1 attgtcctca atataagtga gacgggcatg gccgttgccg cagccgaccc ccttgtagta
       61 gatgactacc tcccacgcat tcgctttcaa ttgcccagct ctgggcagag catcgaaatc
      121 tccgcgcaga ttgtgtggct agcgaaatcg aaaaaaagcg caggcatccg atttgttgat
      181 ctcaccgccg aggcccgcaa tcaaatttca aattggatcg cgtccgaaaa gccagccccg
      241 gaatttgaag gcttaacgcc ggcgcctgtt gagagcctca tccccgcaac cctcgaaagc
      301 ttcccagaag agccaattgg gagtgttttc cctgagcaag accaaccctc ctcgcccgaa
      361 ctgattgcgc acactgcgtc tcaagcatct aagatcgtga cgcctgagat tctggatgcg
      421 aacccgccat cgtttgtcga agatctccag gaaaaagtgc accaccacgc tccagtcgcc
      481 ggttttggac cgcaagttcg aacaggcaaa gtcgagtcgt cagcggatcg catcgagaac
      541 tcgccctcac gcttatatgt tcttgaaata tccggatttc aggtcgccgc agtagtgttt
      601 ttgttcgcgg tgatcagtct caccgttggg ctcacagtgg gacgcggccc gctcggaaag
      661 cgcctccggg acacccagaa atcattaccg gctgttgatg ccacgtcacc ggcactgctt
      721 aatcgcccag gcgaaacgac ttcgcgaaat tccactccgc cagcggcaaa taccttcctt
      781 acgcctgcag taaatccgcc agcgccggcg acggcaggat cgcgctcaga aagcccatcc
      841 gctcaatctc tcaatgcacg accggaagat tcaactaccc gcgtggggcc gatcggacca
      901 tcttcagcaa taacgaaccg atccctcact gattccgata atcccgaagc cagtcctgag
      961 cgcaagggct cgaccgggct aatcgcgcga aacgcgccgc cacccgcgaa ttcccagccc
     1021 gcacattcac ccagggctgt cggcccgata agtggcgcgc cggcaaatcc cgcccaacgc
     1081 agggtaacgc ctgcgacgcg cgcagcgccg cacctgtcat cgccttccac attccttgtc
     1141 actggcccag gcgatggtag caaaccgttc aggctgaatt tgccggaaga accgatcgcc
     1201 gcctcctctt cgttcgcgat gacctcgcaa ctttctgttc ttgttgctcc tgagcccgga
     1261 ccttcagcga ctcataaacc cgctcgattg caagctggtg aacttgtctc gttcgtttgg
     1321 cctcgttatc caaggccggg ggatcgatat ggatcgtcag aaaccgtaaa ggtccgcacc
     1381 accatcggaa agctcggcca agtgctggat ataaagctcg tgagcggctc gatctctctt
     1441 cttcctgctg caatgagcgc cattcgcctg tggcgttaca agcccactct cttgaacaaa
     1501 aggcctgtcc aagttcaaca ggacgtcact attgaattcc agccgctaca gtatttgtcg
     1561 cacgtgccca ctcagcatcc atcgcacaac tagcggcggt ttgagatgtt gattttgcaa
     1621 attaattact taattgtgag agcgactaca gttttgacgt gtcgaaactg caaacgcggc
     1681 ctcgtttgtc aatgtttagg gacgcaatgc gcgacgtaca attgtctata ctccgtcgaa
     1741 tgatatggct cgatcagggt caattccggg gttgggcttg cactaacgtt cacaagtttt
     1801 cgcagcaatc ggaagaacac cctcagtagc tccaaagtgc gtggtctcct tccgagtgcc
     1861 tgataagtcg ggttaccgga caagtgggtc acaggccagt aacggcgatc taggaagtga
     1921 tctctcgtca gccgtaaact acctgcttca accttggatt gacttttcga gcggttgata
     1981 caactcagga agaaagctgg cgcaatcttt tttggtgctc tatcaaaatg caggcgtcca
     2041 tcaccaccgt gattccggcc gcaagggcct tcttcgctgc cacgcggttc tcgattccga
     2101 gctgcatcca gatgaccttg gctccctttg caatcgcgtc atccaccacc ggcggcacgt
     2161 ccggcgcctt gcggaaaatg tccacgatct cgaccggctc cggaatgtcc tgcaaccgcg
     2221 aatagcacgg ctcgccgagc acccgcgtct cgttgggatt cacgccgatc atccggtaac
     2281 ctgcatcttt caggtagacc gcaatttcgt tactcgcccg gaaaggtttt gaggaaattc
     2341 ccacgacggc cacgcacttg tagttcttga gaatttccag caccggatcg ggagccgatt
     2401 ttgcgccctc tggcaacatc acgatcatcg cttggcaagc tccctcttcg cggaaaacac
     2461 cggccaaatc tcgcttatcg cctcgaggaa tcgctcgttc tcttccggcc ttcccgccga
     2521 aacgcgcgcc gcttccggga atcccatcca gcccaggggc cgcacaatca cgccccggtg
     2581 cagcagctca tcgcaaatgc ggctcgtctg ggcgcccatc tccacgaaca caaaattcgt
     2641 ctccgaaggc accggctgca agccgagctt cacaagcccc tcgatgagcc gccgccgctc
     2701 cgcgcggttt gtctccaccg agcgccgcac gtgctccttg tcttccagcg ccgccagtgc
     2761 cgccgcctgc gccacgcccg aagtattgaa cggcgtcttc agcctgttga acgctgccaa
     2821 caaccccgtc ggcccgatcc cgtagccgat ccgcaaccct gcgaggccgt acaccttcga
     2881 aaacgttctc aggatcagga gattcccatg cttccggtac agctcctctg aacgcggcat
     2941 tccaggccgc tcggcgtagt ggatgtaggc ttcgtcgaga acaatcagca cgtctttccg
     3001 cgtccgcgac agaaactcat ccagctcatc cccgccgaac gccgtccccg tcgggttgtt
     3061 gggatttgcc aggtagacga tccttgtctg cggcgtcacg cggcgggcga ttgcggacag
     3121 gtcataggtg tatttccgga gcggcgcctg caaaagctcc gccccggctg cgcgaatggc
     3181 cagcgagtac ggcgcgtaac ttccttccga ggtgaccccc gcaagcccgg gccgcaagag
     3241 acagcgcgcg gccaggtcaa tgatctcgct cgagccaagg ccgacaaaaa tctcctcggg
     3301 cgtcacgccg tgccgcgccg ccagcgcttt gcgcagaagg ttgctcccgc cgtcgggata
     3361 gcggttcgat tcctcgaccg cccgcttcac tgcttccgcg gccatcggcg acggccccag
     3421 agggttttcg ttcgaagcga gcttgattgc atgtattttc agctcgcgct cgacctcttc
     3481 cacgggtctt ccgggaatgt aggcgggcaa gccgcgaatg taatccggga tgagttcttc
     3541 gatcgcgtga gtcatcgggc tggcggaagc tcttcgctcg ttttccttcc tcgcgtggat
     3601 tttgtcgccg gccggcgcag cgcccgccgc aatggacctg tttccgcctc gctcaggatg
     3661 cggtattgtc cctgcggcac gccctccaga gtaatcggcc caatcgcaat gcgcttgaac
     3721 ttctccaccg ggtgttgctc cgcgagcaga acttttcgga gtacgtctct cctggaatct
     3781 cgcagcgtcg cctcgtacca gtaattgacg gcgtggccac gggtcgcgcc gggctggcgg
     3841 atgagccgca acttcacccc ggcgcgcttg gcgaccgaat cgactacggt ttcaggcagc
     3901 cggcccttca ccttcacgta gtacaactgc ggcaggcggt gccactctcg cagcatttcc
     3961 gccgcgagat cgccgtcgtt ggtcaggaac accagccccg acgcatcgta atccagccgc
     4021 ccgaccggat agacgcgtgt cgaaagcccc cgcagacagt tccggagtgt tttccttccc
     4081 tccgggtccg ccatcgtcga aagcacctgc gccggcttgt gcagaagcag cgacactttt
     4141 ctctcttctc cgcgaatcac gtggcccgcc gcttcgatgc ggtccttctc cgggtcggcc
     4201 ttcgtgccga gctgcgtcac cacccggccg ttcacgcgca cttgtccgct caggagcaac
     4261 tgctcggcgt ggcgccgcga agcgatcccc gcccgcgcaa tgatcttctg caatcgttct
     4321 tgcattctgt tttctgtggg aaagacctac ccgagcggcc ttatgtttct aacggatccg
     4381 gcttcgcgag tgttactcgc ctgccgtagc cgctttgggt ccttctccgc ccggctcggc
     4441 attctccgcc ggcatcgctc ccgaattcgg ggcctgcgcc gtttcattcg aaaagcccgc
     4501 ctccccggcg gcttcttcct ggagcgcgat gccttcatcg cccaacgcct cgcgcgccag
     4561 cgcctcgaac tctttcaagc tcggcagctc gtccaggtcg ctcaacccga accgcatcag
     4621 gaactctttc gacgtgcggt acagaatcgg ccgcccaatt acctctttac gccccgccgt
     4681 ggtgatcatt cgtttatcga gcaaggtctt cagcacgccc gcggtattca ccccgcggat
     4741 ctcgttgatc tccggcgccg tcgccggctg cttgtaggcg atcaccgcca gcgtttccag
     4801 cgccggcatc gtcagccgca gcggcggccg taagctcttg atgaaccgcc tcacgacttc
     4861 atggtgctgc ggcttggtgt aaatcttgta gccgccccca accgctcgca cctcgatgcc
     4921 tcggtcgtcg cccgcgcaat tcgaaaccag gtcgtccagc gccgcctgca ccgccagctt
     4981 ttcttctccc agcgcctgag ccagttgctg caccgtggcc ggctcgtccg ccgcgtagat
     5041 aatcgcttcc agcgccgctc ttctctcttc gttcgtcaca aaaccctctt cgccatcacg
     5101 ctctcttttg ttcttgctct tcttcttcct cttctagttg ttttttctac tttcgacttt
     5161 caactgtcaa cttcttgcgc tcctcttcct ttacttcctt tacctccctt acctgctcaa
     5221 cttccttttc ctactgatat tgctcgtcaa tctgccggat tccgccgctc tcgtcgaaca
     5281 ccgcgtcaaa cctcctgtcg cgccgcagca gaatctctcc gaaaggcttt tcttgcacca
     5341 tcgccaccgc ctgcaagcgc accatctcaa gcacagcgag gaacgccacg atcatagcgt
     5401 gccgcgaagg gcaggcttcg aaaaaagcga ccaggctaat cgtgccgccg tcgctggtca
     5461 gcaattgcgc acgcaactga gccatcatct gcgccacggt gaactgctcg tgctgcagat
     5521 cgatccggct cacttccctg cgccgttcga tcacctgctg gaagaccttg atcaggtcca
     5581 tcagcgacac gaccacttcg ccctcggtcg cttcgtcttc gtagagcgtt ttgtccggct
     5641 tcgaccaaac gtgttcttcg atttgctgct tctggtagag aagttgagct gcgcttttga
     5701 atttctcgtg ttccacgagg cgatgcacca aatccgcgcg cgggtccagt tgctcttccg
     5761 ggccgcccag cgggtccggc ggcagcagca tcttcgactt gatgtagatc agcgtcgccg
     5821 ccatgtagac aaaatccccg gaaacatcga tgttcagctc ttcgagcttg tgcaggtaat
     5881 cgagatactg cgccgtgatt ttcgcaatcg ggatgtcgtg gatgttaatc tcctgctttc
     5941 gaatcaagtc caagagcagg tctagcggcc cctcgtagat cggtatctgt atcttgtatg
     6001 gcgtcgccat tcttttcctt cgttagttcg ttacttcact acttcgttgc ttgtgcgcgt
     6061 ttcgccacgg cggctgcaat ttcttcccgc ttcctctccc aaccaatcac cgcttccctt
     6121 acctgctcca tcgtcgcctg cgccacctgg cgcgccttct gcgaccctgc gtcgagaatc
     6181 tccaaaaccc gcgccggatg cttctcatac tcgacgcggc gctcgcgcac gggctcgatc
     6241 cacttgatca gattgtccgc catcgcgcgc ttgcactcaa tacacccgat ctccgccgtg
     6301 cggcagccgt gcgccgccca gtcgagcgtc ttcctgccct cttcgccccg cgcgaacaat
     6361 ttgtgccagt catagaccgg acaaatatcc gggttcccct tgtccgtccg ccgcttccgc
     6421 gccgggtccg tcatcatcgg ccggactttt tcccagatgg cttcgtcgct ctcgctcagc
     6481 gaaatcgcat tgccataact cttcgacatc ttccgcccgt ccgtccccgg aatcctcggc
     6541 gtccgcgtca gcatcacctc cggttccctc aacacggata gcgtgggtat attctcattc
     6601 gcgaaaatag aatggcgata atcaagtggc gctccgcgca tcgcggagtc catcttcgcc
     6661 ggggaagtac ctcctgcatc cttccatgcc gcactgcccg aggtcggata aaaatgcttt
     6721 gcgagcgcag cctcttgttc ctgcgtaaca gcagtccaat tgaccctagc gaatccagca
     6781 ttaaaccggc gcacaatctc ccggctcagc tccacgtgcg agacctgatc ctcgcccaca
     6841 ggcacaatca gcgacttccc ctcttcgccg tacatcacga tgtccgcgca ttgcaggacc
     6901 gggtagccga gaaaaccgta cgtatgcagg tccttgtcct tgatgttctc gagcgcctct
     6961 ttgtaggtcg ggacgcgctc gagccacccc agcggcgtca ccatcgagag cagcaagtgc
     7021 agctccgcgt gttccggaac cagcgactgc aggaaaatca cgcatttctt cggatccagc
     7081 cccgccgcca ggaaatccgt cgcaatgtcc accacgtttt gcgaaatcgc cgaggtgtcc
     7141 gcgtaatcgc tcgtcagggc atgccagtcc gcgatgaaat agaagcactc atattccggg
     7201 tcgtcctgca gcttcaccca gttctgcagc gcgccgaagt aatgcccgat gtgcagccgc
     7261 cccgtcggcc gcatcccgct caccactcgt tttgtcgtcc ccgccgtcaa tccgcccgcc
     7321 tctctcttct tgtcttcact ctgtgatctc ggtgctctct gtggtgaaat ccgtcttctt
     7381 caatctgctc ccaccgtcac ttcctgcgcc ttcgccacct tctccaggat ctcccgcgca
     7441 atgatcatcc tctggatctc gctcgttccc tcgtaaatcg aaaccacccg cgcgtcgcgg
     7501 tacatccgct cgacgtctgt ttcccgcgaa tacccggcgg cgccgtgcac ctggaccgcc
     7561 ttgtagacca ctcgattcac catctcgctc gcgtacagtt tcgctttcga cgcctgcgcg
     7621 cccagccgcc cgcccgaatc cttcagccac gccgcgtgat agaccagccc gcgcgccgcc
     7681 tcgatctccg tctgcatgtc cgcgatcatc cactgaatcg cctggaactc gctgatcgtc
     7741 tttccgaacg cgcgccgctg cttcgcgtac ttcaccgctt cttccatcgc gccctgcgcc
     7801 agcccgacgg ccagcgcccc gatcccgatg cgcccgccat cgagcgtcgc cagcgcgatc
     7861 ttcagcccct gcccctcttc gcccaaacgg ttc
//
LOCUS       DASYVO010000439        12924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_26932, whole genome shotgun sequence.
ACCESSION   DASYVO010000439 DASYVO010000000
VERSION     DASYVO010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 12924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12924
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26932"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VGR03_15130"
     CDS             <1..276
                     /locus_tag="VGR03_15130"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEV1995663.1"
                     /translation="NSFVLQVKGSSMIEDHIMDGDYVVCEQTQVANAGDIVVALIGGE
                     DATLKRFYREGPGKIRLQPANSDMAPIVVAANDVKIQGRVIGVLRKY"
     gene            502..2175
                     /locus_tag="VGR03_15135"
     CDS             502..2175
                     /locus_tag="VGR03_15135"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV1995664.1"
                     /translation="MPSKRQSILIVDPDSASRRVLEELVRSAGYEVAVSSSQAEGFKF
                     VQDASIDLLLLSADLADIQCCDALAEIKGNAATAGTRVILLTQGGGAERARALDLGAD
                     EVLTSPWEPAELLARVRVQLREKRAVDELQEKTRIADQGREMAQTAFQALAVTEKMTR
                     DAFSLGRALKIGVSALFAVAILIAGIFLLYSRRADKEANRAYSVIAQLERGAHRQEQL
                     MADARSARAALQQPDVLEQKQQLKQQSEELRQKISGAGSGDVSLLRKQLEETTTRLKR
                     VETESQSAEKVIRAYAPSVCLLHVSVSFIDRASGRPLRYGGINPDGEPLHDSDGNPVY
                     TLEGRAPEVRADFFGTGFIVGEGRILTNHHVAQPWWKNDELAAVFSQGLNPAVADLTA
                     YFPDATAGVPVSISEISEEADLAVLKGDLSGLKKPILKIDARKEATVSGQPLLSLGYA
                     TGISAILARAGEDAVNEIAKTTGGKPKEVVNELLRRKLIRPLVTQGHIGDVLPDKIVY
                     DAQTTSGGSGGPLINRDGQVIGVTFAVVKGFGGSNFGVPIRFAQPLLKP"
     gene            2241..2441
                     /locus_tag="VGR03_15140"
     CDS             2241..2441
                     /locus_tag="VGR03_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995665.1"
                     /translation="MVHLLVRARVIVVCLLAVLLALPAPVHAYAVLSHEAIIDAAWET
                     HIKRLLSRPTKIIDSLNQKNCN"
     gene            2501..5959
                     /locus_tag="VGR03_15145"
     CDS             2501..5959
                     /locus_tag="VGR03_15145"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF009554.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEV1995666.1"
                     /translation="MKKTKPIRKLLAANRSEIAIRIFRAANELGLRTVAIYSQEDRLA
                     LHRFKADEAYQVGAGKGPVEAYLDIAGIVALAKEKGVDAIHPGYGFLSENPAFARACE
                     KAGITFVGPPPNLLELLGDKTAARRLAASVGVPVLPGTENPIKSVAEAQKIAREIGYP
                     IIVKAAMGGGGRGMRVVHDAAQLNALLEEAQGEARSAFGDASVFLEKYLPRARHLEVQ
                     ILADHHGNLLHLYERDCSVQRRHQKVVEVAPAANLSASIRSELCDAAVQLARKANYRN
                     AGTVEFLYDVDSRKWYFIEVNPRIQVEHTVTEMVTGIDLVRSQILVAQGYKLHEPPMA
                     LPKQENIPLNGAALQCRVTTEDPEKNFAPDYGKISTYRSPAGFGIRLDGGTAYAGAML
                     AAYYDSLLVKVTAWGANLPEACQRMDRALREFRIRGVKTNIPFVENVVNHPKFRAGEV
                     TTSFLDESPELFRFPSRGDRATKLLSYLGDVILNGNPEVKGKQVPKEIEAAVIPAIPA
                     GKPPASTRQLLQKLGPEKFASWARKEKRLLITDTTFRDAHQSLMATRVRTFDLLATAS
                     AIAHRLPNLFSLEMWGGATFDTSMRFLHEDPWQRLRELRVRIPNICFQMLLRGANAVG
                     YASYPDNVIIEFVREAHAQGIDIFRIFDSLNSIENMRVSIDAALETGAVCEAAICYTG
                     DILDKGRPKYSLRYYVAMAKQLEKLGTHFLAIKDMAGLCKPYAAFELVKTLREEVGVP
                     IHFHTHDTSGINSASVLKAADAGVDVADGAIASMSGGTSQPNLNSIVEALRHTPRDTQ
                     LDVGALNECSDYWETVRTYYLPFDSGPKAGSARLYQHEIPGGQYTNLREQAAAMGLGH
                     RWREAEKMYAEVNQLFGDIVKVTPSSKVVGDMTLFLMAKEMQPADILKFDEKHDVPFP
                     NSVVEMFSGVLGVPPGGWPKKLQKIVLRGQAPLRGRPGANLESVKWKEEQESLEKKLG
                     HTLRRDDLLSYLLYSDVFTKYDKFRQTYSDVGVLPTPAFFYGLKSGEEITVEIESGKS
                     LIIKFLTISEPHPDGTRTLFFELNGQPREVNVRDRALRVVERAHPKADPADPGQVAAP
                     TAGVITGIAVQANHAVERGAKLLTLEAMKMQSNIYAPISGRIVKLLVTSGQHVEAKDL
                     LVTIAP"
     gene            5956..6738
                     /locus_tag="VGR03_15150"
     CDS             5956..6738
                     /locus_tag="VGR03_15150"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEV1995667.1"
                     /translation="MSSGQLRKHEQFRSRALRNQRDLIVYTPPGYAGQSWRHFPVLYL
                     HDGQNLFDSATSFIPGQDWHVGQTADHCIQSGAVAPLIIVGMYNTKARIREYTPTHVP
                     KLGGGRADRYAKFLIEEVKPFVDREYRTLSGSQHTGIGGSSLGGLVSLYLGLQHSRIF
                     GKIAALSPSVWWNQLVIHRFAQTATVEPRPRIWLDIGTREGPRIVHDVEKFRDVLLEK
                     GWQPEHDLHYERVEGAEHNEAAWARRVGPFLQFLYPAREAAV"
     gene            6776..7981
                     /locus_tag="VGR03_15155"
     CDS             6776..7981
                     /locus_tag="VGR03_15155"
                     /inference="COORDINATES: protein motif:HMM:NF014298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1995668.1"
                     /translation="MSEGWPLTILCVSSYEKGQEFIRTCKAIGCRVLLLTVEKLRHAE
                     WPFECIDEVFTMPVELPLQKLIYAVSYAARSQPIDRVVALDEFDMENVSALREHLRIP
                     GMGLTTVRYFRDKLAMRARAREAGILVPEFVHVLNYDHLREFMSRVPSPWLLKPRSQA
                     SGIGMKKVHSPEELWPWLDQLGDQQSFYLLEQFVPGAVYHVDSVVSERTIAFAEAHAY
                     GTPPLDTSHQGGVFTTRTLPREAQETRSLLEINRNLLEGLGLMRGVTHAEFLRSHADG
                     KFYFIEVAARVGGAYISDVIETATGINLWREWARLEVGAGNQPYQLPPSRSDYAGVIL
                     SLARQEHPDTSSYTDPEITYRVTKYHHVGFILKSQSHERIQQLLDSYASRFHGDFVAT
                     QPVPDKPSS"
     gene            8109..8942
                     /locus_tag="VGR03_15160"
     CDS             8109..8942
                     /locus_tag="VGR03_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995669.1"
                     /translation="MRVTILLLGCMLATMPASGQQEAKPAAETQSQPGAGQKASAVTA
                     AKPGEFYDKDLDLHFNYPVEMRTLDFSAEMESGHQNIYGVSGENDPEHQKSKRCVRPL
                     LDAELPVDKAPQRAANLEGVWVDDSKEYKESRKPEPIFAKILVMEFLRECLPKKLQKN
                     ENDALGSIALSSVSEPWVQRMPKPLWYEVGKQKIHMNSGVGRPIVNGQLAPAPIFIMS
                     MTTQWRGHLLAWVFTSNDTEVFNEMTKSLVQFGDGTWGPMFAANIGPNGSGTPLTILP
                     K"
     gene            9244..9798
                     /locus_tag="VGR03_15165"
     CDS             9244..9798
                     /locus_tag="VGR03_15165"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEV1995670.1"
                     /translation="MWGNKKPDAPQAGQPEYKNLATNQPSKPASASWEDKPAMSTDAM
                     RPLGATADRATARLGASLHVKGEISGNEDLLIDGSVEGLIQLDERKLTVGASAKVTAD
                     IIAREVVVYGNVKGNLRAKDRIEIKKDGSVNGDLTTSRIMIEDGAYFKGSIEIDKTPE
                     KESGGNAFARSSSAPAGAAATKTI"
     gene            10028..>12924
                     /locus_tag="VGR03_15170"
     CDS             10028..>12924
                     /locus_tag="VGR03_15170"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEV1995671.1"
                     /translation="MGRLRHGVPAALLLSIAAVCASCGGSSSSPPPAPPAADFSLSLS
                     SNSISIVQGAASSAVNVSVNPLNGFSGAVQVALNALPAGVTSNPASPFSVAAGASTPV
                     IFGASPNATTGNFTVSVQGMSGALTHSASLDVAIQSAVNSALPRTAYSRTDSASASDD
                     PFGEPHHRHIAYDPANKHVFIANRAMNRVEVFSIAGQSRVAQVSLPGASSADLSADGT
                     TVWIGTALERIVAIDTLSLRIRNRYSLVGLTPLPGTIFSRPVEVLSLSNGKSMVRLRQ
                     PVSSEALLALWDPASNSLTDLTSTAPVLFQQGVGVLARSGDHSRVLAAANDSSGELAL
                     FDSGGHVVAGPLTLGAGLILRIAANSDGSRFAALFAASGGPQLVLLDASLKQVGAYVP
                     ASAHGVAFSRDGKHLYLSESSSGASFVTVLDGHSAQLLGRVPDAAIQGISSEIEDADE
                     TQLLFGLSNRGISFVDAAAPANLSSTAPAIAAAPSLQPSEGPLAGGTSVVLAGQNFTS
                     AAQLKFGAQSALNVTVSGPTQIQASSPPSVTNGAVNLTAYFQNGWLAIAPDAFSYGPQ
                     ILQLLPNAGMNTGSDSVQIYGYGFGSDPTNITVKIGGANASVQKVESVTSIAASIGLD
                     ASYPFSLQRITLQTPSGSSGQADVFISTPAGSTTSAKSFQFLQSVQSHAKSGFFKFLL
                     YDQKRQRIYLTNVDHLDVFDLQQGTFLAPLQPPGGPPSNAGLRGLALTPDGSQLIVAD
                     FGAKNIYLLDPVKGTGTIVPVGGVPGYTNSGPARVAATSTQTVFVGLSGEGGSSGACS
                     TCLAQMNLTVSPPTIQPAPQPEVTSLTGAPLVQGTAAGDRIFVAFGAAPGGPIAVWNA
                     SAPNQFITSAANASTTDLGAASDGSMFAFQSKSTTEIRAADLCLISVPTSAELDQIPG
                     RLLVPGVALHPSGALIYQPFLTGASGRAGVKGGIDILDAHSGALRLRILLP"
BASE COUNT         2625 a         4096 c         3433 g         2770 t
ORIGIN      
        1 aattcctttg tcctgcaggt caagggcagc tccatgatcg aagatcacat catggacggg
       61 gactatgtcg tttgcgagca aacccaggtc gccaatgccg gtgacatcgt tgtggcgctg
      121 attggcggcg aagatgcaac gctcaagcgc ttctatcgcg aaggccctgg caagatccgt
      181 ctgcagcctg caaattccga catggctccc atcgtggtgg ccgcgaacga cgtcaagatt
      241 cagggccgcg tcatcggcgt ccttcgcaaa tactagttct gaatcagatg actgttctcc
      301 ctcccgaacc atagcgattg gcaagcttga tcggtccccc tccgtcatct ttccgaggat
      361 aaattatttg tgtcagaatc cagttgcctg catcagaagg gtgtccacgg tattcccgtg
      421 aatccttgtg acgcatcccg gcagggaaga taagtccgaa ggaaggctcg gcgggacatc
      481 taaggctctg aggtcgcgaa cttgccctcg aaacgtcaaa gcattctgat tgtcgatcct
      541 gactcggcct cgcgccgcgt gcttgaggaa ttggtgcgtt cggccggcta tgaagtggcc
      601 gtatcgtctt cccaggctga aggattcaag tttgttcaag atgccagcat cgaccttctg
      661 cttctctccg ctgaccttgc cgacatccag tgctgcgatg ccctcgccga aatcaaaggc
      721 aatgcggcca ctgcggggac acgcgtgatt ctcctcacgc agggcggggg agctgagcgg
      781 gctcgggccc tcgatctcgg cgcggacgag gtacttactt caccgtggga gccggcggaa
      841 ttgctcgcgc gcgttcgcgt ccagttgcgc gaaaaacggg ctgttgacga actccaagaa
      901 aaaactcgga tcgcggacca gggccgcgag atggcgcaaa ccgctttcca ggcgcttgct
      961 gtgaccgaga agatgacccg cgacgctttt tccctgggac gggctttgaa aattggagtc
     1021 tctgccctct tcgccgtcgc catcctgatc gccggaatct ttcttctcta ttcccggcgt
     1081 gccgacaagg aagcgaatcg cgcttactcc gtgatcgccc aactcgagcg tggcgctcac
     1141 cgccaggagc aattgatggc ggatgcccgc agcgctcgcg cagctcttca gcaacccgat
     1201 gttctcgagc aaaagcagca gctcaagcag caatcggagg agttgcgcca gaaaatctcg
     1261 ggcgccgggt ccggcgacgt gtccttgctc cgcaagcagc tcgaggaaac caccacccgg
     1321 ctcaaacgcg tggagaccga gtcgcaatcc gcggagaaag tgatccgcgc ttatgcgcca
     1381 agcgtatgcc tcctccatgt atccgtctcc ttcatcgacc gggcttcagg ccgccccctg
     1441 cgttacggcg ggatcaatcc ggatggcgag ccactccacg acagtgatgg caacccggtt
     1501 tataccttgg aaggccgcgc acccgaagtt cgcgctgatt tcttcggcac cggattcatc
     1561 gtcggcgaag gccgcattct caccaatcac cacgtggcgc aaccgtggtg gaagaatgac
     1621 gagcttgccg ccgtttttag ccaggggctg aatcccgccg tcgctgatct gacggcctat
     1681 ttcccggacg cgactgccgg cgttcccgtc agcatttccg aaatctccga ggaagcagac
     1741 ctcgcagtgc tgaaaggtga tttatccggt ttgaaaaaac ccatcctgaa aatagatgcc
     1801 cgcaaagaag cgacagtcag cggccagcct ctgctctccc tcggctacgc cacgggcatt
     1861 agcgccattc tggcgcgtgc tggagaagac gccgtgaacg aaatcgccaa gaccacgggc
     1921 ggaaaaccga aagaggtcgt caacgaactc ctccgccgta aacttatccg ccctctggtg
     1981 actcagggcc atatcggcga cgttcttccg gacaaaattg tctatgatgc gcagaccact
     2041 tccggcggct cgggcgggcc gctcatcaat cgcgatggac aagtgattgg cgtgacattt
     2101 gcggttgtca aaggttttgg cggttcgaat tttggcgttc ccattcgctt cgcccagccg
     2161 ctcctcaagc catgagggcc cgttttgttt cattctcaga tgtggagccg atacggcgtc
     2221 ttccctcgtc atctggaatc atggttcacc tgcttgttcg cgcccgtgtg atcgtcgttt
     2281 gcctgctcgc cgttctgctg gcgttgccgg ctcccgttca cgcttacgct gtcctctctc
     2341 atgaagccat catcgatgca gcttgggaga cgcacatcaa gcgtctcctt tcccggccca
     2401 caaaaataat tgattctcta aatcaaaaaa actgcaactg agtcgcgccg ccactatgaa
     2461 acaatgtaga atttcgcgcg acgcattgat cgcctctgag atgaagaaaa ccaaaccgat
     2521 ccgcaaactc ctcgccgcca atcgcagcga aatcgccata cgcatcttcc gcgccgccaa
     2581 cgagctcggc ttgcgcaccg tggccatcta ttcgcaggaa gaccggctcg ccttgcatcg
     2641 cttcaaggct gacgaagcgt accaggtcgg cgccggcaag ggtcccgtgg aagcctatct
     2701 ggacattgcg ggaatcgtcg cactggcgaa ggagaagggc gtcgacgcca tccacccggg
     2761 ttacggtttt ctttccgaga atcctgcgtt cgcccgtgcc tgcgaaaagg ccggcatcac
     2821 cttcgttggt ccgccgccga atcttctcga attgctgggt gacaaaaccg ctgctcgaag
     2881 actggccgca tcagtgggtg tgccggttct gcccggaacc gagaatccga ttaagtcggt
     2941 agcggaagcg caaaagattg cgcgggagat cggctatccc atcattgtga aagcggccat
     3001 gggcgggggt ggccgcggca tgcgcgtcgt tcatgacgcc gcgcagctca atgctcttct
     3061 ggaagaagcc cagggagaag cgcgttccgc ctttggcgat gcgtctgttt tcctggaaaa
     3121 atatttgccg cgcgcacggc acctggaagt tcagatcctc gccgatcatc acggcaactt
     3181 gctccatctt tacgaacgcg attgctctgt gcagcgccgc catcagaaag tcgttgaagt
     3241 ggccccggcc gccaatctct ccgccagtat ccgcagcgaa ctctgcgatg cggcggtgca
     3301 gctcgcgcgc aaggccaact atcgcaacgc gggaaccgtc gagtttctct acgacgtcga
     3361 ttcgcggaag tggtatttca tcgaggtcaa cccccgcatt caagtcgagc acaccgtgac
     3421 cgaaatggtc acgggcatcg atctggtgcg ctcgcagatt ctcgtcgcgc aaggttacaa
     3481 gctacatgag ccgccgatgg cgctgccgaa gcaggagaat atcccgctca atggcgcggc
     3541 tcttcagtgt cgcgtgacaa ctgaggaccc ggagaaaaac ttcgctcccg actatggaaa
     3601 aatttccacc tatcgttccc ccgccgggtt cggtattcgt ctcgatggcg gcaccgctta
     3661 tgccggcgcg atgctcgccg cctattacga ttcgcttctc gtcaaagtta cggcctgggg
     3721 cgcaaatctt cccgaagctt gtcaacgtat ggaccgcgcc ctccgcgaat tccgcattcg
     3781 cggtgtgaaa acaaatatcc ctttcgtcga aaatgtcgtg aaccacccaa agtttcgcgc
     3841 cggcgaagtc accacctctt tcctcgacga atcgcccgag cttttccgtt tccccagccg
     3901 cggcgaccgc gccaccaagc tcctttctta tctcggcgat gtcatcctca acggcaatcc
     3961 ggaagtgaag ggcaaacagg tcccgaaaga aatcgaagca gcagtcatcc cggcaatacc
     4021 cgcgggcaaa ccgccagcca gcacccgcca gcttctgcaa aaactcggcc cggagaaatt
     4081 cgcctcctgg gcgcgaaaag aaaaacgcct gcttatcacc gacacgactt tccgcgacgc
     4141 tcatcagtcg ctcatggcta cgcgcgtccg cacgttcgat ctgctcgcta ccgccagcgc
     4201 catcgcgcat cgcttgccca atcttttcag tctcgaaatg tggggcggcg caacttttga
     4261 cacttcgatg cgcttcctgc acgaggatcc ctggcaacga ctgcgcgaac tgcgcgtgcg
     4321 cattcccaat atctgtttcc agatgctcct ccgcggcgcc aacgccgtcg gttacgcctc
     4381 ctatcccgac aatgtcatca tagaatttgt ccgcgaagcg cacgcccagg gcatcgacat
     4441 cttccgcatc ttcgattcgc tcaattccat cgagaacatg cgcgtttcga tcgatgctgc
     4501 actcgaaacc ggcgccgttt gcgaagccgc catttgctat accggcgaca tcctcgacaa
     4561 gggccgtccg aaatactcgc tcaggtacta cgtcgcaatg gccaagcaat tggaaaaact
     4621 gggcacgcac ttccttgcca taaaagatat ggccggcctt tgcaaacctt acgcagcatt
     4681 cgaattggta aaaacgctgc gtgaggaagt cggcgttccg atccacttcc acacccacga
     4741 cacgagcggc atcaactcgg cctccgtgct gaaagcagcc gatgccggag ttgacgtcgc
     4801 cgacggcgca atcgcctcta tgagcggagg caccagtcag cccaatctca attccatcgt
     4861 cgaagcgctc cgccacacgc cacgcgacac gcagctcgat gtcggcgcgc tcaacgaatg
     4921 ctccgattat tgggaaaccg ttcgcaccta ctatcttccg ttcgactccg gtcccaaggc
     4981 cggctcagcg cgcctctacc agcatgaaat tcccggcggt cagtacacca acctgcgtga
     5041 acaggctgcc gccatgggcc tcggtcatcg ttggcgcgaa gccgaaaaaa tgtatgccga
     5101 agtaaaccag cttttcggcg acatcgtcaa agttacgcct tcgagcaaag tcgtcggcga
     5161 catgacgctt ttcttgatgg ccaaggaaat gcagccggca gacattctca agttcgacga
     5221 aaaacacgac gtgccatttc caaattcggt tgtcgaaatg ttttctggcg tcctcggcgt
     5281 gccccccggc ggctggccga agaagttgca aaaaatcgtc cttcgcggcc aggcgcctct
     5341 ccgtggccgc ccaggtgcaa acctggaatc agtgaaatgg aaagaagaac aggaatccct
     5401 cgagaaaaaa ctcggtcaca ccctccgtcg cgacgacctc ctgagctatc tgctttattc
     5461 cgacgttttc accaaatacg acaaatttcg ccagacctat tcggacgtcg gcgttttgcc
     5521 cacgcccgca tttttctatg gtttgaagtc cggcgaagaa atcaccgtcg aaatcgaatc
     5581 cggaaagtcc ctcatcatca agtttctgac catcagcgaa ccgcatcctg atggcacgcg
     5641 cacgctcttt tttgaattga acggccagcc ccgcgaagtg aacgtccgcg atcgcgcgct
     5701 gcgtgtggtc gaacgtgcac atccaaaagc ggaccctgcc gatcccggcc aggtcgctgc
     5761 tcccaccgcc ggtgtcatca ccgggatcgc cgtgcaagcg aatcacgctg tggaacgcgg
     5821 cgcaaaactt ctgactcttg aagccatgaa aatgcagtcc aatatctatg cgccaatttc
     5881 aggacgcatc gtgaagctac tggtgacctc cggacagcac gtcgaagcga aagatctcct
     5941 ggtgaccatc gcaccgtgag ttccggacag ctccgcaaac acgaacaatt ccgctcgcgc
     6001 gcgctccgca atcagcgcga cctcatcgtt tacacgccgc ccggatacgc cggccagtcc
     6061 tggcgccatt tccccgtcct ctatcttcat gacggccaga atctcttcga tagcgccact
     6121 tccttcatcc ctgggcaaga ctggcacgtc gggcagaccg cggatcattg cattcaaagt
     6181 ggcgcggtcg ccccgctgat catcgtcggg atgtacaaca ccaaagcgcg cattcgcgaa
     6241 tacactccca cccacgtacc caaactcggc ggtggccgcg ccgaccgcta cgcaaaattt
     6301 ctcatcgaag aagtgaagcc cttcgtcgat cgcgaatacc gcacgctctc cggctcgcag
     6361 cacaccggca tcggcggttc gtcgcttggc ggcttggtct ccctctatct ggggctccag
     6421 cattcgcgca tttttggaaa aatcgccgct ctctcgccat cggtctggtg gaaccagctc
     6481 gtcatccacc gttttgccca gacagcaacc gtcgagcccc gcccgcggat ctggctggat
     6541 atcggcacgc gcgaaggccc gcgcatcgtg catgacgtcg agaaattccg tgacgtgctc
     6601 ctcgaaaaag gctggcagcc ggagcacgac ttgcactacg agcgcgtcga aggtgcggag
     6661 cacaacgaag cggcttgggc gcggcgcgtc gggccatttc tgcaatttct gtatcccgcg
     6721 cgcgaggccg ctgtataatc cgcgctctag tctcgtactt ttcagggggc gtcgaatgtc
     6781 ggagggctgg cctctaacca ttctctgcgt cagttcctac gaaaaaggtc aggaattcat
     6841 ccgcacctgc aaagccatcg gctgccgtgt ccttctcctg accgtcgaaa aacttcgcca
     6901 cgccgaatgg cctttcgaat gtatcgacga agtcttcacc atgcccgtag agcttccgct
     6961 gcagaaattg atttacgccg tgagctatgc ggccaggtcg cagcccatcg atcgcgtcgt
     7021 cgcgctcgat gaatttgaca tggagaatgt ctcggcactg cgcgagcatc tccgcattcc
     7081 cggcatgggt ctcaccaccg tccgctactt tcgcgacaag ctggctatgc gcgcgcgggc
     7141 ccgcgaggct ggcatcctcg tgcccgaatt cgtccacgtt ctcaattacg atcatcttcg
     7201 agaattcatg tcccgcgttc ccagtccctg gctgctcaag cctcgctcgc aggcttccgg
     7261 tatcggcatg aaaaaggttc attctccgga ggaactctgg ccctggctgg accaactcgg
     7321 cgatcagcaa tcgttttact tgctcgaaca atttgttccg ggcgcggtct accacgtcga
     7381 cagcgtggtt tcagagcgca ctattgcttt cgccgaagct cacgcctatg gcacgccgcc
     7441 gctcgatacc tctcatcaag gcggcgtttt caccacgcgc actcttcctc gtgaagcgca
     7501 agaaaccaga tcgctcctcg aaatcaatcg caatctgctg gaaggccttg gcctaatgcg
     7561 cggagtgacc catgctgaat tcctccgctc gcacgcggac ggcaaatttt atttcatcga
     7621 agtcgccgcc cgcgtcggcg gcgcctatat ctccgatgtc atcgagaccg ccactgggat
     7681 caacctctgg cgcgagtggg cgcggcttga agttggggca gggaatcagc cttaccagct
     7741 tccgccgtca cgcagcgact acgccggagt cattctttct ctggctcgtc aggaacatcc
     7801 cgatacttcc tcctacacag atcccgaaat cacctatcgc gtcacgaaat atcaccacgt
     7861 cggcttcatc ctgaagtccc aaagccacga acgcattcag cagttgctcg attcctacgc
     7921 cagccgtttt cacggcgatt tcgttgccac ccagccggtc cccgacaaac caagttctta
     7981 gcaccaggac agaattactg ggccatcgga atctggcctg cgccctgcta caatctgctg
     8041 ttgaaatgag agccaagcca atcaaaggaa agatcggtac gcgaggagtt cgcgatggtc
     8101 ttccagccat gagagtgacg attctccttc ttggttgcat gttggcaacg atgcctgcga
     8161 gtggccagca agaagccaag ccggccgcgg agacgcagtc gcagcctgga gcgggacaaa
     8221 aggcaagcgc tgtgacagcg gcgaagcccg gcgagttcta tgacaaggat ctcgacctgc
     8281 acttcaacta tccggtcgag atgcgtactt tggatttcag tgccgagatg gaaagcggcc
     8341 atcaaaacat ctacggtgtc tccggcgaga acgatcccga gcaccagaaa tcgaagcgtt
     8401 gcgttcgacc tctcctcgat gccgaactgc cggtggacaa ggcgccgcaa cgggccgcca
     8461 atctcgaggg agtttgggta gacgattcga aagagtacaa ggagtcgcgg aagccggagc
     8521 cgatcttcgc gaagatattg gtgatggagt ttctccggga gtgtttaccg aaaaagttgc
     8581 agaagaacga gaacgatgct ctaggctcca tagcgctaag ctccgtctcc gagccctggg
     8641 tccagcgtat gccgaagccg ctatggtatg aggtcggcaa gcaaaaaatc cacatgaatt
     8701 ccggcgtcgg tcgacccatc gtcaatgggc aactggctcc tgcgccaatc ttcatcatgt
     8761 ccatgacgac gcagtggcgt ggtcacctgt tggcgtgggt gttcacctcg aacgatacgg
     8821 aagtttttaa tgagatgaca aagagcttgg tgcagtttgg tgatggtact tggggcccta
     8881 tgtttgcggc gaacatcggc cccaacggct cggggactcc gttgaccatt cttccgaagt
     8941 gagattggct gtcgaaaaat cgcgtttgca ctcataacct caaaatcggc ccgtcacatg
     9001 gctctgtagt tgcataatcg gaaactaacc acccacccca cactccgctt tacattcccc
     9061 ccgcattcca tcacaatcct tttgtactgc ccacctagcc ctgcaacgcc tgtcccaaag
     9121 tctagttact gtctggaacg atgcgttcac actttgtttg tcacgcgcag tgccgaggaa
     9181 tacagtttgc agtaccccgg ccggaaatct gcgtggggtg gttctgtccc ttggggaggt
     9241 ccgatgtggg gcaataagaa accggatgca ccgcaagcag ggcaaccgga gtacaaaaat
     9301 ttagcaacga atcagccgtc gaagcctgct tcggcgtctt gggaggataa acccgcgatg
     9361 agtacagatg cgatgcgtcc attgggggca accgccgacc gcgccactgc gcggctgggg
     9421 gctagcctgc acgtaaaagg tgaaatctcc ggcaacgaag atctgttgat cgatggctcg
     9481 gtcgaaggac tgatccagct cgatgagcgg aagctgactg tcggtgcctc ggccaaagta
     9541 accgccgaca tcattgcccg cgaggtggtg gtgtacggca acgtgaaggg caaccttcgc
     9601 gccaaggatc gaattgaaat caagaaggat ggttcggtca acggtgacct gactacttcc
     9661 cgcatcatga tcgaagacgg cgcctacttc aaaggctcca tcgaaatcga caagactcca
     9721 gaaaaggaat ccggtggcaa tgcatttgcc cgtagctctt cggcacctgc gggcgcagcg
     9781 gcaaccaaga cgatctagct ttcatccact ttcaacgatt ctttcgacaa cggcgctccg
     9841 gtcggggcgc cgttcgtttg ttagctggct attggactct cttatccatt aattcttaac
     9901 ttgacaagta gtttagtcag tgtatcgttg ccccgggtat cctcttggcg tagcgcgccg
     9961 tccggtctcg accctccttc gtctcggagc accagcggtt cgcgcggaag gttcgcgggg
    10021 tcatctcatg ggacggttac gtcacggcgt cccggcggcg cttctcctct ccatcgcggc
    10081 ggtgtgtgcg agttgtggtg gctcatcttc aagccctccg cctgctccgc ctgctgctga
    10141 tttctccctg agcttgtcat cgaattcgat ttccatcgtg cagggcgctg caagttccgc
    10201 cgtcaatgtc tccgtgaacc ctctgaacgg tttttccggc gccgttcaag tagcactcaa
    10261 cgcgcttcct gccggcgtca cctcgaatcc cgcgagtccc ttcagtgttg ctgccggcgc
    10321 gagcacccct gtcatttttg gcgcctcccc aaatgcaact accgggaatt tcacggtctc
    10381 cgtgcagggc atgagcggcg ctctaacaca ttcggccagc ctggatgtcg cgatccaaag
    10441 cgctgtgaat tcagctcttc cgcgcaccgc ctattcgagg acggattcgg cttcagcttc
    10501 agacgatcct tttggtgaac cgcatcatcg ccacatcgct tatgatcccg cgaacaagca
    10561 cgtcttcatc gccaatcgcg ccatgaatcg cgtggaagtg ttttccatcg ccggccaatc
    10621 tcgtgtcgcg caagtttccc tacccggcgc cagcagcgcc gacctctccg ccgatggcac
    10681 aaccgtttgg attggtaccg cactggaacg catcgtcgca atcgatacgc tctccttgcg
    10741 catcaggaat cgctactcct tggtggggct cactcctctg ccgggcacga tcttcagccg
    10801 tcccgttgag gttctttctc tttcgaacgg caagtccatg gtgcgcctgc gacaacccgt
    10861 ctcctcggaa gccctgctgg ctctctggga ccccgcgtcg aattcgctca ccgatcttac
    10921 ttccaccgcc cctgtgcttt tccagcaggg cgtgggcgtc cttgcccgct ccggcgatca
    10981 ttccagggtc cttgccgctg ccaacgactc cagcggggaa ctagccctct tcgattcggg
    11041 aggccacgtc gtcgctggcc cgctcacact cggcgcaggt ttgatcctta ggatcgccgc
    11101 caactccgac ggtagccgct tcgccgcgct tttcgccgca agcggaggcc cgcaacttgt
    11161 attgctcgat gcttccctca aacaggttgg cgcctatgtt cctgcatccg ctcacggtgt
    11221 cgcgttctct cgcgatggaa agcatttgta tctcagcgag tcttcttccg gagcgtcttt
    11281 cgttaccgtt ctcgacggtc atagcgcgca actcctcggc cgagtcccgg atgcggcgat
    11341 tcagggcatc tcctcggaaa tcgaggacgc ggacgagacg cagctcctct tcggcctttc
    11401 caatcgtggt atcagctttg tcgatgctgc ggcgccagca aatttgtcct cgaccgctcc
    11461 cgccatcgct gccgcgccaa gtcttcagcc ttcggaaggc cccctcgccg gcggaacttc
    11521 cgtcgtcctt gctggccaga atttcacttc cgctgctcaa ctgaaattcg gtgctcaatc
    11581 cgcgctgaac gtcaccgttt ccggaccaac tcaaatccag gcgagctctc cgcccagcgt
    11641 cacgaacggc gccgtcaacc taactgctta tttccagaac ggctggcttg ccatcgcccc
    11701 ggacgccttc agctacggtc cgcaaattct gcagctcctc ccaaacgccg gcatgaatac
    11761 cggcagcgac tcagtacaaa tctacggcta cggcttcggc agcgatccaa caaatatcac
    11821 cgtgaagatc ggcggcgcaa atgcatccgt gcaaaaggtg gagagcgtta cgagtattgc
    11881 tgcttccatc ggtctcgacg ccagctatcc tttttccctc cagcgcatca ccctgcagac
    11941 gccctccggt tcttcgggcc aagcagatgt tttcatttca acccccgcgg gttctacaac
    12001 gtctgccaaa tcattccagt tcctgcagag tgttcaatcg catgcgaaat ctggcttctt
    12061 caagttcctg ctctacgatc agaaacggca gcgcatctat ctgacaaacg tcgatcacct
    12121 cgatgttttc gatctccagc agggcacatt cctcgctccg ctccagcctc cgggcggtcc
    12181 tccctctaat gcggggttgc gcggcctggc gctgactccc gatggttctc agctcatcgt
    12241 cgccgatttc ggcgcgaaaa atatctatct cctcgatccg gtgaagggaa ctggcaccat
    12301 cgtgcccgtc ggcggagttc ccggctacac aaactctggt cctgcgcgcg ttgccgcgac
    12361 cagcacgcaa acagtcttcg tcggtctcag cggcgaaggt ggctcttctg gcgcgtgttc
    12421 cacatgtctc gctcagatga acctgaccgt gagcccgcca accattcagc ccgctccaca
    12481 gccggaagtc accagcctca ctggcgcgcc gctcgttcaa ggcacggctg cgggcgatcg
    12541 catttttgtt gccttcggcg ccgctcccgg tggtcccatc gccgtatgga acgcgagtgc
    12601 cccaaatcag ttcatcactt ctgccgccaa cgcctcaacg acggatctgg gagccgcgtc
    12661 cgacggcagc atgtttgcct tccagtcaaa gagcacgacc gaaatacgcg ccgcggatct
    12721 ttgtctcatt tccgttccca cttccgctga gctcgatcaa atcccggggc gtttgctggt
    12781 gcccggtgtt gctctgcatc cttcaggcgc cttgatctat caacctttcc tgaccggtgc
    12841 ttctgggagg gctggcgtga agggcggcat cgacatcctc gacgcgcatt cgggcgccct
    12901 ccgtttacga attcttctgc ctca
//
LOCUS       DASYVO010000440         4038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_126122, whole genome shotgun sequence.
ACCESSION   DASYVO010000440 DASYVO010000000
VERSION     DASYVO010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126122"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VGR03_15175"
     CDS             <1..273
                     /locus_tag="VGR03_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995672.1"
                     /translation="KVLGVVITLFDKRTNISKDTHGQIRSVFGEVLFKTKITKNVRLE
                     ESPAYKETILTYAPKSPGAIEYKKLAGEVIQRVEEARVTRHAEDAA"
     gene            227..1084
                     /locus_tag="VGR03_15180"
     CDS             227..1084
                     /locus_tag="VGR03_15180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV1995673.1"
                     /translation="MSKRLGLPVTLKMRHDAHYVESLTSYSGAAIGRMIRVDQIRPNP
                     DQPRKALGDLRELTESIREKGVLEPLLVRYVPREDCFHIISGERRYHASRAAGLREVP
                     CIEKTADDAETLEIGLIENIQRKDLTPFEEADGLHRLSTQFEYTHEDVAKKIGRARSS
                     VTETLSLRNIPESLRKKCVEHGILSKSLLLQIARQPTEKRMIEMFHRIMQGGLTRDEA
                     RRERREEHAGPQRPQPFIFQFEPPAETFKFRIQFKKSHVSRDELISTLHEILAQLEGS
                     SSEADSTAA"
     gene            1128..1493
                     /locus_tag="VGR03_15185"
     CDS             1128..1493
                     /locus_tag="VGR03_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995674.1"
                     /translation="MKTLRLQGDSMVELSPQQMQIIERLFAVGFRPIAIPPYESALCM
                     RKGDCAAVLAAVPSGGIKLLAPPSYLVDGNLSVKLKRVAGEVFVWKKKEVEATPEKLK
                     ELESFRRELTEILDLPPKQ"
     gene            1544..2515
                     /locus_tag="VGR03_15190"
     CDS             1544..2515
                     /locus_tag="VGR03_15190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HEV1995675.1"
                     /translation="MEPYDIVCIGAGPTGLATAIEAKRAGMRPLVIDKACLCHSIFRY
                     PVNMVFFTTPELLEIGDLPLVCAAEKPTRIEALKYYRKTAEHYTLELRLYERVSRVDG
                     QNGNFTVVTSTENGPEQRYQSKKIAVATGYYDLPNQLDVPGEDLPHVSHYYTEPHEYW
                     NQDVVVIGGKNSSAEAALDLYRNGARVTLVHRNKELGASIKYWVRPDIENRIKAGQVK
                     ALFETRVKEITNDEVMVENGAGEKRLPARQVFAMTGYHPDYNFIESLGVQLDPESRKP
                     ALNPSTLESNVPGLYLAGVVTGGRHTGEIFIENGRFHGKQIIQALTG"
     gene            2584..3060
                     /gene="nuoI"
                     /locus_tag="VGR03_15195"
     CDS             2584..3060
                     /gene="nuoI"
                     /locus_tag="VGR03_15195"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEV1995676.1"
                     /translation="MASIIREVGETFGALAKGFSVTFRNMFRKTVTENYPYEPVHFQP
                     RYRGIHVLHRDEGGLEKCVGCFLCAAACPANCIYIEAAENVDANRISAGERYASVYNI
                     DYSRCIFCGYCVEACPTDAITHGHGFELATYDINSLVYRKEQLLEIGAAAATPSGR"
     gene            complement(3405..3722)
                     /locus_tag="VGR03_15200"
     CDS             complement(3405..3722)
                     /locus_tag="VGR03_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995677.1"
                     /translation="MNHYRTQPPWEFLLSASRNSLQSYELSRLSHAANLRKEIGALLD
                     LWMEENSSAMLARWLMEQRERPQHTQEDAPSGETLPKNGHAVSDNFFADRSVTPPANR
                     GPA"
BASE COUNT          925 a         1061 c         1250 g          802 t
ORIGIN      
        1 aaagttctgg gcgtggtgat tacgcttttt gacaagcgta cgaatatttc caaggacacg
       61 cacgggcaaa ttcggtcggt gttcggggaa gtgctcttca agactaaaat caccaagaac
      121 gtgcggctgg aagagagtcc ggcctacaag gaaacgattt tgacgtatgc accgaaatca
      181 cccggtgcaa tagagtacaa gaaactcgca ggggaggtta tccaacgtgt cgaagaggct
      241 cgggttaccc gtcacgctga agatgcggca tgatgcacat tacgtagaat cgctgacaag
      301 ctatagtggc gcggcgatcg ggcgcatgat tcgcgtggac cagatccgcc cgaatcccga
      361 ccagccgcgc aaggcgctgg gcgacctgcg cgagctcacg gagtcgattc gcgagaaggg
      421 cgtgctggag ccgttgctgg tgcgctacgt cccgcgcgag gactgctttc atatcatctc
      481 cggcgagcga cgctatcatg cgtctcgcgc ggcggggctg cgcgaagtgc cgtgcatcga
      541 aaaaaccgcc gacgacgcgg agacactcga aatcgggctg atcgaaaata ttcagcggaa
      601 agatctgaca ccgtttgaag aagcagacgg gctgcaccgg ttgagcacgc aatttgaata
      661 cacgcatgag gacgtggcca aaaagattgg ccgggcacgg tcctcggtga ccgaaacttt
      721 gtcgctgcgc aatattcccg aatcgctgcg gaagaagtgc gttgagcacg ggattctttc
      781 caagtcgctc ttgctccaga ttgcgcgcca gcctaccgag aagagaatga tcgagatgtt
      841 tcatcgcatc atgcagggcg gactgacgcg cgatgaggcg cgccgcgaac gccgcgaaga
      901 gcacgcggga ccgcaaaggc cgcagccgtt catctttcag ttcgagccgc cggcggagac
      961 gttcaagttc cggattcagt tcaagaaaag tcatgtttcg cgcgatgagc tgatttctac
     1021 gctgcacgaa atactggcac aactcgaggg atcctcctcg gaagccgatt cgacagcggc
     1081 ttagttattc ccaagaacgg gccgggcatt tcgagaagac tcaaggcatg aaaaccctgc
     1141 gcctgcaagg cgactccatg gttgaacttt cgccgcagca aatgcagatc atcgagcggc
     1201 tcttcgcggt cggctttcgc cccatcgcga ttccgcctta cgagagtgcc ctgtgcatga
     1261 ggaaaggcga ttgcgcagcg gttttggctg ccgttccgag cggcggaatt aaactgctcg
     1321 cgccgccatc ctatcttgta gatgggaacc tcagtgtgaa gctgaagcgt gtcgcaggcg
     1381 aagtgttcgt ctggaaaaaa aaggaagtgg aagccacgcc ggagaaattg aaagaacttg
     1441 agtcgttccg gcgggaactc acggaaatcc tggatctgcc gccgaagcag tagcgttgcg
     1501 gcaatttgca attccgaaaa actcccggca aactgaaatt ctcatggaac cctacgacat
     1561 tgtctgcatt ggagcggggc cgacgggcct ggcgaccgcc attgaggcca agcgcgccgg
     1621 aatgcggccg ctggtcatcg acaaagcctg cctgtgccat tcgatttttc gctatccggt
     1681 gaacatggtg tttttcacga cgccggagct gctcgaaatt ggcgatcttc cgctggtttg
     1741 tgccgcggag aagcccactc gcatcgaggc gctgaaatac taccggaaga cggcggagca
     1801 ctatacgctt gaattgcgcc tgtacgagcg ggtgtcgcgg gtcgatggcc agaatgggaa
     1861 ctttacggta gtaacatcaa cggaaaatgg accggagcag agataccaga gcaagaagat
     1921 tgccgtggcg acaggttatt acgatctgcc gaaccagctg gacgtgccgg gcgaggatct
     1981 tccgcacgtt tcgcattact acacggagcc acacgaatat tggaatcagg acgtggtcgt
     2041 gattggcgga aaaaattctt ccgcggaggc ggcgctcgat ctgtatcgca atggcgcgcg
     2101 cgtgacgctg gtgcaccgaa acaaggaact tggcgcgtcg atcaagtatt gggtgcggcc
     2161 ggacattgag aaccggatca aggccggtca agtgaaagcg ttgttcgaga cgcgcgtgaa
     2221 agagatcacg aatgacgaag tgatggtcga aaatggagcc ggtgagaaga gattgccggc
     2281 gcgacaggta ttcgcgatga ctggatatca tcccgattac aattttattg agagcttggg
     2341 cgtgcagctc gatccggaga gccgcaaacc agcgctgaat ccgagcacat tggagagcaa
     2401 tgtgccgggt ctttatctcg cgggcgtggt gaccggcgga cggcataccg gcgagatttt
     2461 cattgaaaat gggcggttcc atgggaaaca gatcattcaa gcactgacgg ggtaagctgc
     2521 atggggaagt ggcacccggg ccagatttgc tcgccccgaa cccgtttgct aagctcttag
     2581 tttatggcat cgatcatccg ggaagtgggc gagacgtttg gcgcgctggc gaagggcttc
     2641 agcgtaacgt ttcgtaatat gtttcgtaag acggttacgg agaactatcc gtacgagccg
     2701 gtgcattttc agccgcgcta ccgcgggatc cacgtcctgc accgcgacga aggcggactg
     2761 gaaaagtgtg ttggctgctt cctctgcgcg gccgcctgcc cggcgaattg catttatatc
     2821 gaagccgcgg agaacgtcga tgcgaaccga atctccgcgg gcgaacgcta cgccagcgtg
     2881 tacaacatcg attattcgcg ctgcatcttc tgcggttatt gcgtggaagc ttgcccgacc
     2941 gatgcgatca cgcacggtca cggcttcgag ctcgcgacct acgacatcaa ctctctcgtt
     3001 taccgcaaag agcaacttct tgaaatagga gccgctgccg cgactccgtc tggccgctag
     3061 tacttaccct ctctattctc gcgattgttt ccgggtgact ccgacgtttt ccagatttat
     3121 cgccggacat ctcttgccgt ctctctgaat tagcgtccca taacataaag cagccaagac
     3181 gtaagtccgg agggaggcgc gtagacgtga tggatgcgat gcttgttgaa ggaagacacc
     3241 aagcgcggtt gtgcgaagcg tttaacaaga acgctggggc gagagagtgg aagccgctag
     3301 ggcgcatgac gctaagcaca cttccggggc cgagcgaccg cggcttcgtg agcgcaagag
     3361 ctagcggcaa actccggatt gagcgccgaa aaatagcctt cacctcaagc ggggccgcgg
     3421 tttgcgggcg gcgtgaccga ccgatccgca aaaaagttat cggacacggc atgtccgttt
     3481 ttcgggagag tttcgccgga gggagcgtct tcttgcgtgt gctgcgggcg ttcccgctgc
     3541 tccatcagcc agcgcgccag catggccgag gaattctcct ccatccaaag gtcgagcagc
     3601 gcgccaatct ccttgcggag attggcggca tggctgagac gcgagagttc atagctctgc
     3661 agcgagtttc gcgaagccga gagaagaaac tcccacgggg gttgggttct gtagtggttc
     3721 acgcgagcct cctggtagct gacctccgta cgttaacact tcgtaaatca cagtcaagag
     3781 taacgacagg gtaatgagct gtggaaaagt gtagaaaact ttggcggtcg gagcacgaga
     3841 ctgctgatag tcatggactg caggccgaag tttactcgat cgatggcgct gctggggcga
     3901 cggggatgcg cgcttgcagc gcctgcgcgg gggattacga agacccggac cgtacggcgt
     3961 ggacgccgga cgatgcggaa gatgacgagc gcgacgatgc accattcgca accacagaag
     4021 agtttcctgc tgaggacc
//
LOCUS       DASYVO010000441         2948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_148143, whole genome shotgun sequence.
ACCESSION   DASYVO010000441 DASYVO010000000
VERSION     DASYVO010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148143"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1758)
                     /gene="hutU"
                     /locus_tag="VGR03_15205"
     CDS             complement(70..1758)
                     /gene="hutU"
                     /locus_tag="VGR03_15205"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEV1995678.1"
                     /translation="MSLTVPGIAAEAGLAKGPFRAPRGTAISCKGWQQEAALRMLLNN
                     LDPEVADKPEDLVVYGGTGKAARNWECFHAMVRSLKALEADETLLVQSGKPVGIFRTH
                     EYAPRVLLCNSNLVGHWNNWDRFHELDRAGLMMYGQMTAGSWIYIGTQGILQGTYETF
                     AAAAKKHFGGNLAGKLVVTGGMGGMGGAQPLAATMNGAAFLGIDVDPERIKRRVKAGY
                     CDVMVTSLDEALRILKNAVRKGEATSVGLVGNCADLIPELAKRGVVPDLLTDQTSAHD
                     PIDGYVPNGMTLEAALELRAKNPEEYKKHSMAAMAQHVQGMLDLQKLGAVTFDYGNNI
                     RTFAFEQGVKNAYDFPGFVPEYIRPLFCEGKGPFRWAALSGEASDIARTDQLVLELFP
                     QDEHLNRWIKLAQKRVKFQGLPSRICWLGYGERDKFGLALNDLVAKGELKAPIVIGRD
                     HLDCGSVASPFRETESMLDGSDAIADWPLLNALINTASGASWVSIHNGGGVGIGYSQH
                     SGQVTVADGTKEMAKRIERVLTNDPGMGVVRHVDAGYPEAIDFAKKHGVKVPMS"
     gene            1860..2189
                     /locus_tag="VGR03_15210"
     CDS             1860..2189
                     /locus_tag="VGR03_15210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1995679.1"
                     /translation="MVEVREYLDRGGRSPFAAWSDRLNREAAVKVAAALARIQQGNFS
                     SARSVGAGVHEYRIDFGPGYRIYFGKDGDRLVILVGGGTKKRQQEDIATALARWQDYK
                     QRKGTGR"
     gene            2195..2578
                     /locus_tag="VGR03_15215"
     CDS             2195..2578
                     /locus_tag="VGR03_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995680.1"
                     /translation="MALTRDFKETIRARVKRDPGFRKALLREGIENFLSGDVETGKII
                     LRDFINATVGFTKLSDVTHRSAKSLMRMLGPRGNPQARNLFEIVAYLQQAEGVRFEVR
                     SARAALRAKRRPSSQRGSRAAVAGH"
     gene            2754..>2948
                     /locus_tag="VGR03_15220"
     CDS             2754..>2948
                     /locus_tag="VGR03_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995681.1"
                     /translation="MNDPLSKPLEEIRQVAKDSGLHIFFGWLAERNDALAVHWNEDNG
                     GDWKGFLGCARAVSRVPGRRF"
BASE COUNT          614 a          990 c          733 g          611 t
ORIGIN      
        1 ccgcgtccgc gcagcgtcag gagttgcgat gcaccggtaa ttagcagtga actcggcaaa
       61 gcttacgtat tatgacatgg gaacttttac gccatgcttc tttgcgaaat cgatggcctc
      121 gggatacccc gcatccacgt gccgcaccac gcccatcccc ggatcattcg tcaacacgcg
      181 ctcgatacgc ttcgccattt ccttcgtccc atccgccacg gtcacttgtc cggaatgctg
      241 cgaatatcca atccccacgc cgccgccgtt gtggatggac acccacgacg cgccgctagc
      301 cgtattgatc agcgcgttca gcagcggcca atccgcaatc gcgtccgatc catccagcat
      361 cgattccgtc tcgcgaaatg gcgacgccac cgatccgcaa tccagatgat cgcgcccaat
      421 cacgatcggc gccttcaatt cgcccttggc caccagatcg ttcagcgcca ggccgaactt
      481 atcccgttcg ccataaccca gccagcagat gcgcgacggc agcccctgaa acttcactcg
      541 cttttgcgcc aatttgatcc agcgattcaa atgttcgtcc tgcggaaaca gttccagcac
      601 cagctgatcc gtccgcgcaa tgtccgaagc ttcccccgaa agcgccgccc accggaacgg
      661 tcctttcccc tcgcaaaata atggccgtat gtactctggc acaaaaccgg gaaagtcgta
      721 agcgtttttc acgccctgct cgaacgcaaa cgttcgaatg ttatttccgt agtcaaacgt
      781 caccgcgccc agcttctgca aatccagcat tccctggacg tgctgcgcca tcgccgccat
      841 cgaatgtttt ttgtactctt ctggattctt cgcgcgcagc tccagcgccg cctccagcgt
      901 catcccattc ggcacgtagc cgtcgatagg atcgtgcgcg ctcgtctggt ctgtcagcaa
      961 atccggcacc accccgcgct tcgccagttc cggaatcaag tccgcgcaat tgcccaccaa
     1021 tccaaccgaa gtcgcctcgc ctttgcgcac cgcgttcttc aaaatccgca gcgcctcatc
     1081 cagcgaagtc accatcacgt cgcaatagcc cgccttcacg cgccgcttga tgcgctccgg
     1141 gtccacatcg atccccagaa acgccgcccc attcatcgtc gccgccagcg gctgcgcccc
     1201 gcccatgccg cccatcccgc cggtcaccac cagcttgccc gccagattcc cgccaaaatg
     1261 ttttttcgcc gccgccgcaa acgtttcgta cgtcccctgc aaaatcccct gcgtgccaat
     1321 gtaaatccag cttcccgcgg tcatctgtcc gtacatcatc aatcccgcgc ggtcgagttc
     1381 gtggaagcgg tcccagttat tccagtgccc caccagattc gaattgcaca gcagcacccg
     1441 cggcgcgtat tcatgcgtcc ggaaaatccc caccggcttc cccgattgca ccagcagcgt
     1501 ctcatccgcc tccagcgcct tcagcgaccg caccatcgca tgaaaacatt cccaattccg
     1561 cgctgccttc cccgtcccgc cgtacaccac caaatcttcc ggcttgtccg ccacctccgg
     1621 atcgagattg ttcaagagca tccgcagcgc cgcctcttgc tgccacccct tgcaagaaat
     1681 cgccgtcccc cgcggcgcgc gaaacggtcc cttcgccaag cccgcctccg ccgcaattcc
     1741 tggaaccgtc aagctcatga aatttcctct ccgccgctca tactaaccca cgccactccc
     1801 ttttgcacag gccgcttagc tgacccttga caatggtatc gtatgtgtta ccattagcta
     1861 tggtcgaagt gcgcgaatac cttgaccggg gtggacgcag cccttttgcc gcttggtctg
     1921 accgtctgaa cagagaagcg gcagttaagg tggcggctgc tttagcacgc atccaacaag
     1981 gcaatttttc cagcgccagg agcgttgggg ccggtgttca cgagtaccgg attgattttg
     2041 gtccgggcta ccggatttac ttcggcaaag atggcgaccg gcttgtaatc cttgttggcg
     2101 gtggaacgaa aaaacgccag caggaagaca tcgccactgc actggctagg tggcaagact
     2161 acaaacaacg aaaagggaca ggaaggtgac tatcatggct ttgacacggg atttcaagga
     2221 aacaattcgg gcgcgcgtaa aacgtgatcc tggtttccgc aaggcgcttc tccgggaggg
     2281 catcgaaaac tttctttccg gtgacgtgga aaccggcaaa atcatactgc gcgatttcat
     2341 caatgccaca gttggcttta ccaaactgag cgacgttacc catcgttcgg cgaagagtct
     2401 gatgcgcatg ctcggtcccc gtggcaaccc tcaggctcgc aacttgttcg agatcgttgc
     2461 ctatttgcag caagcggaag gcgtgcgctt cgaagtccga tccgcgcgtg ccgccttacg
     2521 cgcgaagcgc cggccatctt ctcagcgagg aagccgagca gccgtcgcgg gccactgaat
     2581 actcgaaccc gcgaccccac cgtgacagtg agcgattcat aaacacttgc gaggacttac
     2641 atgagcacgg acggcatcgc aagtcattgc aagtatgtct aaggcagggt tattgtgtac
     2701 ctcgatgtgt accacgcctg gagtggtgct accatctggt tcatcggacg cgcatgaacg
     2761 atcctctctc aaagccacta gaagaaatcc gtcaggtcgc gaaagacagc ggcctgcata
     2821 ttttctttgg ttggttagct gagcgcaacg acgcactggc cgttcattgg aacgaagaca
     2881 acgggggaga ctggaagggc ttcctcggat gcgcgagggc agtcagccgg gtgcccgggc
     2941 gtcgtttt
//
LOCUS       DASYVO010000442         7031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_218744, whole genome shotgun sequence.
ACCESSION   DASYVO010000442 DASYVO010000000
VERSION     DASYVO010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218744"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..2506
                     /locus_tag="VGR03_15225"
     CDS             353..2506
                     /locus_tag="VGR03_15225"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEV1995682.1"
                     /translation="MAKRASNSAGDSPGAKVKRAAKTAQREYVPIEDDPTAAGSAPEG
                     ALEASSDGAAKPSMQEVFGPGGFLEKCMKGGFDPSTVSSDYEHRPGQLEMAELVHDAF
                     ESHHHAIVEAGTGTGKTLAYLLPAICSGRRVVISTATKSLQEQLYQKDIPFLQKHFAP
                     NLKVAVMKGRANFLCISKLNQMADQAMLKGMEELDAFRQIKQWSKLTETGDRAELTFL
                     PDDSELWGKLDARRDTCTGKNCPSFNPCFVTSMHQRAQEADLIIVNHHLFFADLALKQ
                     DDFGSILPEYSAVVFDEAHEMEDVASDYFGRQISNFRFEELARDADQTMRLLHLGTPS
                     LLRKTQRIRERCRGFFELFPPRDGRFSFLRNEREAFLEQNREAYDSLVTSLKSMETEF
                     AALTAKPEELTRIARRSFELRQELAFLFESNERNFVYWYERRNKGVFLTATPIDVSQI
                     LRERLFEKFDTVILTSATLTVGGRFDYIRQRLGIDHAKDRALPPEFDFPKQALLYLPR
                     KMPDVRDAGFPAKAADEIVRLLELSQGRAFCLFTSYSQMNDLFERVRSRVPFPLLLQG
                     TAPRSALLERFKNTEAAVLFATASFWQGVDVPGEQLSCVIVDRLPFAVPSDPIVAARV
                     RALQEDGRNPFAEFQVPQAVLALKQGFGRLIRTKTDRGVLALLDTRLQRMPYGKIFLE
                     SLPRYGVTCELADVARFLDGNGDSKRLGASSASPS"
     gene            2562..2942
                     /gene="queD"
                     /locus_tag="VGR03_15230"
     CDS             2562..2942
                     /gene="queD"
                     /locus_tag="VGR03_15230"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEV1995683.1"
                     /translation="MFQVSVEETFSAGHALRGYRGKCENVHGHNYRVCVTLEGPQLDS
                     IGLLVDFTQLKQVIREIMGRLDHQFMNDLEPFKTVNPSAENVAKYFYDEVTRQLKDLP
                     PGARVTDVVLWETDTSWAQYRPGA"
     gene            2966..3154
                     /locus_tag="VGR03_15235"
     CDS             2966..3154
                     /locus_tag="VGR03_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995684.1"
                     /translation="MNRAFLIIMIPALLVAAGYIVVLRYMGIAPGYPRLVVAMILFFG
                     MIYWLSRRTGRKVNSSRV"
     gene            3240..3932
                     /locus_tag="VGR03_15240"
     CDS             3240..3932
                     /locus_tag="VGR03_15240"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV1995685.1"
                     /translation="MVITEIFKSIQGEGTRAGLPCIFVRLTGCNLRCTWCDTAYAFHG
                     GKKMSVEEVLARVDELAGRREMPGSQASFPLVELTGGEPLLQEEIVPLAEKLLAAKYG
                     VMIETSGERFIGGLPREVIKIVDVKCPDSGEPDTFETRNLEALSENDEVKFVLSTRRD
                     YEFAREFMAQHGLARRVKQVLFSPVSEDPQGKWPGLEPRMLVEWILADGLPVRLGLQL
                     HKFIWDPATKGV"
     gene            4015..4740
                     /gene="queC"
                     /locus_tag="VGR03_15245"
     CDS             4015..4740
                     /gene="queC"
                     /locus_tag="VGR03_15245"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEV1995686.1"
                     /translation="MELEQDRRKAVVLLSGGMDSCVSTAIARERHGASNIALLHASYG
                     QRTQERERRAFDDIANFYGVRERLAVQLDHFRAIGGSALTDKNISVPENELGAPGPHG
                     STIPVTYVPFRNAHFLSVAVSWAEAIGAGAIYIGAVAEDSSGYPDCRLEYYRVFQELI
                     GVGTRPETQIEIVTPVIMLKKSAIIRRGIELGAPLNLTWSCYQNEDTACGACDSCLLR
                     LRAFAEAGVPDPIPYRRTVSAGG"
     gene            4832..5977
                     /locus_tag="VGR03_15250"
     CDS             4832..5977
                     /locus_tag="VGR03_15250"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1995687.1"
                     /translation="MKSFYKSWALAAILMAALASVLAPRAAAQGDGAIRGQILDVAGK
                     PWAELGIQAVSEQGAKLDTKTDKDGNYVFRNLRSGIYTVYVQLPAPNKPYEAKCQVAG
                     GSEAKVDLNFKDIASKQGAEYQEAAKKAEETKGKLEGLKAHFTAGNALIDQEKQAKID
                     LQKAPADQRDAAKQKLADLGDQAVKEFQEAAKSLVEKDPNAHLVWFKLGEAYDTAGRN
                     DDAAQAYQQAITAKADVPGYYNNMGNVLARAGKIDEAKAAYTKSAELDPPNAATAWRN
                     FGISLYNANRLGDAVEPLKKSAELDPKNPQTWYLLGASLVYKMTTKKVGDRVEVQFAP
                     GTIEAYQKAVELDPNGTWGQQAKQGLEQLQLMAPGIDTKVNTKKKKS"
     gene            complement(6036..6851)
                     /locus_tag="VGR03_15255"
     CDS             complement(6036..6851)
                     /locus_tag="VGR03_15255"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1995688.1"
                     /translation="MIRIVRELWLFFWRDLSIARTYRTVFVLEAIEALFGAAMFYYVA
                     RFVDTPALQHALPQGGSYFAFSLVGFVFLDYLNAALDTFDRSLEEARDSGTLEHLLIT
                     QTSLPVLVAGSAIYPFVATTLRIVVYVAWAAALFGFPLGTANWLAVFAVLLVTLLAFS
                     GLGILSASYLLLFKRGNPAKWFFLGVSSVAGGMLFPVSILPDWLQLVARLNPVTYALD
                     AMRASLLGGAGVVALWRPLAILLLFATVLLPLSMSVFAWSLRRTKITGTLTHS"
     gene            complement(6848..>7031)
                     /locus_tag="VGR03_15260"
     CDS             complement(6848..>7031)
                     /locus_tag="VGR03_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995689.1"
                     /translation="HTLPEVELLADRVAVIDAGRLLACDTLSTLKLSTGASSLEEVFL
                     RLTGNDAAASSGDRAL"
BASE COUNT         1604 a         1841 c         2316 g         1270 t
ORIGIN      
        1 taacggcaca gaccgtgccc ttgggcccgc tgcaggaaga caaggaaaac gcggcgagca
       61 agagaaccgg aagaagaaat ttgctgcgat tcatgtggca ccccacgaca gactagtcta
      121 cgtttgcccc ctggaaagga aaacgctcaa aatccccaca gccgactgta acgcgatggg
      181 cgagcgggtg ggcactgcaa aaggaaccag cgtgttcaac tcttaaatgc cctgaatcaa
      241 gagagataga atgtccagcc gcctgtgggc gagctgctga cggagcgaag agaaagggta
      301 tgttatcgta actggtctct cggtttcgtt aggtgagagt gaaggcggat ttttggcgaa
      361 gcgcgcatcc aattcggcgg gggattctcc gggagcgaag gtcaagcgtg cggcaaagac
      421 tgcccaacgc gagtatgtgc ccatcgagga tgaccccacg gcagcgggaa gcgcgccgga
      481 gggcgctctt gaggcatcgt ctgatggagc ggccaaaccc tccatgcagg aggtgtttgg
      541 gccaggcgga ttcctcgaaa aatgcatgaa gggcggattt gacccatcca cggtcagctc
      601 cgactacgaa catcgacccg ggcaactgga gatggcggaa ttggtacacg atgcgtttga
      661 atcgcatcat cacgccatcg tggaggcggg cacgggcacg ggcaaaacgc tggcgtattt
      721 gctgccggcg atttgcagcg gacggcgcgt ggtgatttcc acggcgacga agtcgctgca
      781 ggaacagctc taccagaagg atattccttt tctgcaaaag catttcgcgc cgaacctgaa
      841 agttgcggtg atgaaggggc gcgcaaattt tctgtgcatc tcgaagctga accagatggc
      901 ggaccaggca atgctgaagg ggatggagga gctggatgca ttccggcaga tcaagcagtg
      961 gtcgaagctg acggagaccg gcgaccgcgc ggagctgaca tttctgccgg atgattcgga
     1021 gctttgggga aagctggacg cgagaaggga cacgtgcacg ggcaagaatt gcccgtcgtt
     1081 caatccctgt tttgtgacga gcatgcacca gcgcgcccaa gaagccgacc tgatcatcgt
     1141 gaatcaccac ttgttttttg cggacctcgc gctgaaacag gacgatttcg gcagcatcct
     1201 gccggaatac tcggcagtgg ttttcgacga agcgcacgag atggaagacg tggccagcga
     1261 ctatttcggg cggcagattt cgaatttccg gttcgaagag ctggcacgcg atgccgatca
     1321 aacgatgcgt ttgctgcacc tgggcacgcc atcgttgctg cgaaagacgc aaagaatcag
     1381 ggaacgttgc cgcggttttt tcgaattgtt tccaccgcgt gacgggcggt tctcgtttct
     1441 gaggaacgag cgtgaagcat ttctggagca gaaccgcgaa gcctacgatt cgctggtgac
     1501 gtcgctgaaa agcatggaga cggaatttgc ggcgctcacg gcaaagccgg aagagctgac
     1561 gcgcatcgca cggcggagct tcgagctgcg ccaggagctg gccttcctgt tcgaatcgaa
     1621 cgagcggaat ttcgtctatt ggtacgagcg gaggaacaaa ggcgtattcc tgacggccac
     1681 gccgatcgac gtcagccaga ttctgcgcga gcggctgttc gaaaaattcg acacggtgat
     1741 tctgacctcc gcgacgctca ctgttggtgg gcgcttcgac tacattcgcc agcgactggg
     1801 catcgatcac gcgaaggatc gcgcgctgcc gccggagttc gattttccca aacaggcgct
     1861 actctacctg ccgcgcaaga tgccggacgt ccgcgacgca gggttcccgg cgaaggccgc
     1921 ggatgaaatc gtgcggttgc tggagctgag ccagggccgg gcgttttgcc tgttcaccag
     1981 ttattcgcag atgaatgatt tattcgagcg ggtgcgttcg cgcgtgccgt ttccgctgtt
     2041 gttgcagggc acggcgccac gctccgcgct gctggaacga tttaagaaca ccgaggccgc
     2101 ggtgctgttc gccacggcaa gcttctggca gggcgtggat gtgcctgggg agcagctatc
     2161 ctgcgtcatc gtcgaccggc tgccgtttgc ggtgccgagc gacccgatcg tggcggcgcg
     2221 agtgcgcgct cttcaggaag acgggcgcaa tccgtttgcg gaatttcagg tgccgcaggc
     2281 ggttctggcg ttgaagcaag gatttgggcg tttgatccga acgaagacag accgtggcgt
     2341 gcttgcgctg ctggataccc gcctccagcg catgccctat ggtaaaattt tcctggagtc
     2401 gctgccccga tacggggtga cgtgcgaact ggccgatgtc gcccggtttc tcgatgggaa
     2461 cggcgactcg aagcggctgg gggccagttc ggcaagtccc tcctgaaggg tcacgatccc
     2521 gggagatccg cagcaagtgg gttgcgcagt tctggagggc catgtttcaa gtaagcgtag
     2581 aagagacgtt ttcggcagga catgccctgc gcggttaccg gggcaagtgc gagaacgtgc
     2641 acggccacaa ctatcgcgtg tgtgtgacgc ttgaagggcc gcagctcgat tcgatcgggc
     2701 tcttggtgga cttcacgcag ctcaagcagg tgatccgcga gatcatgggc aggctcgacc
     2761 accagttcat gaacgatctc gaacccttca agacggtgaa tccttcggcg gaaaacgtgg
     2821 cgaagtattt ttacgatgag gtcacgcggc aattgaagga tctgccgccg ggcgcgcggg
     2881 ttacggacgt cgtgctgtgg gaaacggata ccagctgggc gcagtaccgg cccggggcct
     2941 agttgtatgg aaaacgggtg agccgatgaa tcgagctttt ttgattatca tgattccagc
     3001 gctgctggtg gcggcgggct acattgttgt gctgcggtac atgggaatag cgccagggta
     3061 tccgcgactg gttgtcgcga tgattctttt ttttgggatg atttactggt tgtcgcgccg
     3121 gacggggcgc aaagtgaatt ccagccgggt atagattgca aaggcctggg ccgggcagag
     3181 caggaaaagc ggcaactgag ccccgacagg aactggggta aaccgccgca ctccaagaca
     3241 tggtcatcac ggaaattttc aagtcgatcc aaggtgaagg cacgcgcgcg ggcctgccgt
     3301 gtatattcgt gcgcttgacg ggttgcaatt tgcgatgcac gtggtgcgat accgcttatg
     3361 cgtttcatgg cgggaagaag atgagcgtcg aggaagtttt ggcgcgcgtg gatgaactgg
     3421 cgggacgccg ggaaatgccc ggctcacaag cttcctttcc gctagtggaa ttgacgggcg
     3481 gggaaccatt gttgcaggaa gaaattgttc cgcttgccga gaaactgctg gccgcgaagt
     3541 acggtgtgat gatcgagacc agcggggagc gattcatcgg aggcttgccg cgagaagtca
     3601 tcaagattgt ggatgtgaag tgtcccgatt cgggggagcc ggatacgttc gagacgcgca
     3661 atctcgaggc gctgtcggaa aacgatgaag tgaagttcgt gctttccacg cggcgcgatt
     3721 atgaatttgc gcgagaattt atggcgcagc acgggctggc gcggcgggtg aaacaggttt
     3781 tgttttcgcc ggtgtccgag gatccacagg ggaagtggcc gggcctcgag ccgcgcatgc
     3841 tggtggaatg gattctggct gatggactgc cggtgcggct cgggctgcaa cttcacaagt
     3901 ttatctggga cccggcgacc aagggcgttt gaagccaacg ggagaaacaa atttaacgca
     3961 gagacgcaga ggacgcagag aagaagagaa aaatcaggaa gaactgaata ggcaatggaa
     4021 ttagagcagg ataggcgaaa agcggtggtg cttttgagcg gcgggatgga ttcgtgcgtg
     4081 agtacggcga ttgcgcgcga acgccacggc gcgtcaaaca tcgccttgct gcacgccagc
     4141 tacggccaac gcacccagga gcgcgaacgg cgcgcgttcg atgacattgc taatttctac
     4201 ggcgtgcgcg aacggcttgc cgtgcaactc gatcatttcc gcgcgatcgg tgggtccgcg
     4261 ctcaccgaca agaacatttc cgttccggaa aatgaattgg gggcgcccgg cccgcatggc
     4321 agcacgattc ccgtgacgta cgttcccttc cgcaatgcgc attttctttc cgtggcggtg
     4381 agctgggcag aagcgatcgg cgcgggagcg atttacatcg gggcggtggc ggaagacagc
     4441 tccggctatc cggattgccg gctggaatac taccgggtgt tccaggaatt gatcggcgtg
     4501 gggacacggc cggaaacgca gatcgaaatc gtaacgccgg tcatcatgct gaagaagagc
     4561 gcgatcatcc ggcgtggcat cgaactgggc gcaccgctga atctaacctg gtcgtgctac
     4621 cagaacgaag ataccgcctg cggcgcttgc gatagctgcc tgctgcgctt gcgcgccttc
     4681 gcggaagccg gcgtgccaga ccctatcccc taccgccgaa ccgtttctgc cgggggctga
     4741 gcttggaatc agcccgcatg agagagataa tgggacgcac atagcctgga cactccgggc
     4801 ggggctcccc cgtgaaaaag gagaatggag catgaagagt ttttataagt cgtgggcgct
     4861 cgccgcaatc ctgatggccg cgctggcaag cgtacttgca ccgcgcgccg cagctcaggg
     4921 tgacggcgcc atccgcgggc agatcctgga tgttgccggc aagccgtggg cggagctcgg
     4981 cattcaggct gtcagtgaac agggcgccaa acttgatacg aagacggaca aggacgggaa
     5041 ttacgtcttc cgcaatttaa gatcaggcat ttacacggta tacgttcagc tgccggcgcc
     5101 caataagcct tacgaggcga aatgccaggt agcgggcggc tcggaagcca aggtagacct
     5161 aaatttcaag gacatcgcct ccaagcaggg cgccgaatat caggaagccg cgaagaaggc
     5221 ggaagaaacg aaaggcaaac tcgagggtct caaagcgcac tttacggcgg gcaacgctct
     5281 catcgatcaa gagaaacagg cgaagattga tctgcagaag gcacccgcgg atcagcgcga
     5341 tgcggccaag cagaagttgg ccgacttagg ggatcaggcg gtcaaggaat tccaggaagc
     5401 tgcgaagagc ctggtggaga aagatcccaa cgcgcatctg gtgtggttca agctcggaga
     5461 agcttacgac accgcgggcc gcaatgacga cgctgctcaa gcgtaccagc aggccatcac
     5521 cgccaaggcc gacgtgccgg ggtattacaa caacatgggc aacgtgctgg cacgcgcggg
     5581 aaagatcgat gaagccaaag ctgcgtacac gaagagcgcg gaacttgacc cgcccaatgc
     5641 cgcgacggct tggcgcaact ttgggatcag cctgtacaac gcgaatcgtt tgggcgatgc
     5701 tgtcgaaccg ttaaagaagt ccgccgagct cgatccaaag aatccacaaa cctggtatct
     5761 cctgggagct tcgctggttt acaagatgac cacgaagaaa gtcggcgaca gagtagaggt
     5821 ccagtttgcg ccgggaacca tcgaagccta ccagaaggcg gtcgagctcg accccaacgg
     5881 aacatggggc cagcaggcca agcaggggct cgaacagctt cagttgatgg ctccgggcat
     5941 cgacaccaaa gtcaatacga agaaaaagaa gtcctagtca agatcggggc agttttttcg
     6001 ggccggttcc gcgacgcggg gccggccttt ttttgtcaac tatgcgtcag ggtgccggtg
     6061 atttttgtgc ggcgaagcga ccaggcaaag acgctcatcg aaagcggcaa gagcacggtg
     6121 gcaaagagaa ggagaatggc gagagggcgc caaagagcca ccacgcccgc ccctcccaga
     6181 agcgaggcgc gcatcgcgtc cagcgcatag gtgaccggat tgagtcgcgc gaccaactgc
     6241 agccaatccg gcaagatgct gaccgggaaa agcatgccgc ccgcgacgct ggaaactccc
     6301 aggaaaaacc atttggcggg attgccgcgc ttgaaaagca gaagataact cgcggaaaga
     6361 atcccgagcc cggagaaggc gagcagcgtg accaggagga cggcgaagac cgcgagccag
     6421 ttcgcggtgc ccaacggaaa gccaaagagc gccgctgccc aggcgacgta gacgacgatg
     6481 cgcagcgtgg tggcgacgaa aggataaatg gctgagccgg cgacaagaac ggggagcgaa
     6541 gtttgcgtaa tcagaagatg ttcgagcgtg ccggagtccc gcgcttcctc cagactgcga
     6601 tcaaaggtgt ccagggcggc gttgagataa tccaggaaaa cgaaaccgac gagcgaaaaa
     6661 gcaaaataac tgccgccttg cggcaaggcg tgctggaggg cgggcgtgtc cacgaaacgc
     6721 gccacgtaat aaaacatggc ggcgccgaag agcgcctcaa tggcttccaa gacgaatacg
     6781 gtgcggtagg tgcgcgcgat gctgaggtcg cgccaaaaga aaagccacaa ctcgcggacg
     6841 atgcggatca taaggcgcgg tctccgctgg atgcagctgc atcgttgccc gtcaagcgaa
     6901 gaaagacttc ttcgagcgag gaagcgccgg tgctcagttt gagagtggag agcgtgtcgc
     6961 aggccagaag gcggccggcg tcaatcacgg ccacgcgatc ggcgagaagc tcgacctcgg
     7021 ggagagtgtg c
//
LOCUS       DASYVO010000443         6844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_234491, whole genome shotgun sequence.
ACCESSION   DASYVO010000443 DASYVO010000000
VERSION     DASYVO010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234491"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VGR03_15265"
     CDS             complement(<1..737)
                     /locus_tag="VGR03_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995690.1"
                     /translation="MKTRILCITLLLFLPLAAAAQTADEIVKKALDARGGVEKIKAVQ
                     SERVSGHVSFSGGMEGTFVVELKRPLKMHAEISIEGQKIIRVYDGKSSGWTVNPFTEA
                     KNVQPLSPEDLKNITDESDFDGPLVDYKAKGNQIELAGKENLDDKPVFRLKLTNKNGD
                     VRFYIFDASSFLLLKWAGIQKTGDQALPWESFFSDFHEVQGLKYPFRIEQGSPGTDIK
                     QTLTAGKIEIDPQIDDSRFAKPAPPDAP"
     gene            965..4027
                     /locus_tag="VGR03_15270"
     CDS             965..4027
                     /locus_tag="VGR03_15270"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV1995691.1"
                     /translation="MKSRAITVGFLLICLGFCGGAWCQQEPAGEGRPITPAGTLVIDA
                     ATHLPAVGAMPMAMLRSPDALGHGGKGRYLLVVNSGYGVQFSEDTNEAQQSIAVVDLN
                     ATPEPLVIQNVYFPSPQSANIGLAFSSAAGADGNFEMYVSGGFENKVWIFRFDPKAAR
                     PVQPGSNGPDTKVAAPSFEISKAGEVSRSDYNKGKAALYPTGLAVTPDGTTLVTANNL
                     GDSVTIVRGLRGARRMERVDLHHPGKLSENIYPYGVVVLGSGKSARAYVSCWNDSSVA
                     VIPLSGKSVVREYIIVDRHPTAMTPNAGGTRLFVSNSDADSVSVIDTTTDQEIERIDV
                     RLAEDALPGASPEAVALSEDEKTLYVANAHSNAVAVVALSEKARGGKTVQKMAEAAKS
                     KILGFIPTGQYPSAVALADGKLFVGNGKGTGFESSSMRVNNSGRTPNPPNAAFPPNKE
                     KNGQGGQYSGSIVSGNISVVPLPDGPALAHYTQQTMQNDGLMDFAPPKLFAGNSPIKH
                     VIYIIKENRTYDQVFGDVKTSGDGHAADGEADLAIFGNGATAQRPDGTRQAVTPNHHA
                     LAQRFGLFDRFFVNSEASPDGHNWSTAAFSTDYVDKGFRWEYSERGRTYDWEGFNRLP
                     DYEPPSNLQVEKFKGDVLAALTDLLEKHLPYHQGFMDLAEPKSLYLWDAAARAGLTYR
                     NYGEFVTVISTKDVEAAGQKRKKSYPDVSNAVRAIPNKESLQKHHSPSFRSFDVTAPD
                     SMTVDCYKAALDLSASSDPAVTRANSNAKCRGNSRFGEWLAEFKAFAEDREAGRDDAM
                     PALSVLRFPNDHTTGMKNGFPTPQFMVADNDYAVGRLVEAVSSSVYWKDTAIFIVEDD
                     AQAGPDHVDSHRSLGLAISAYNRPGALIHKFHSTVSMIRTIEMLLGIAPMNQLDASAI
                     SMDIFQETPDLTPYKAVLPTIAADNLMTRKPKDKTTAEWMKKSNRQNFAHADMADPKV
                     LNAVIWFACTGNGSNLPQAAQLPAYQAMRLGIAKVDDDESVSQKDDDD"
     gene            complement(4151..5272)
                     /locus_tag="VGR03_15275"
     CDS             complement(4151..5272)
                     /locus_tag="VGR03_15275"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEV1995692.1"
                     /translation="MKRTFLLLFAFLLAAATAPAQTPPPPLISPEVHADKSVTFRFRA
                     PNDKEVAVMIEGVPKPFPMQKDDQGVWSVTTDPLAPDYYGYTIIADGVGLFDPSNHAI
                     KPNFLYRASELHVAGPATFSWEIAAVPHGEIHHHFYKSGVVGDDRGYFVYTPPGYDPR
                     GKQTYPVLYLLHGFSDDASAWTAVGRANVILDNLIAQGKAKPMLVVMPLGYGAPEVLL
                     PNSGVFSDRALTDRNFDKFREALLTEVIPRVESAYLVKKDRNSRAIAGLSMGGSESLL
                     TGLNTLDKFGWIGAFSSGGITEEFDKEFPALDSKATEQLHLLWIACGTDDRLIDINRK
                     VRVWLASKNIKHVDIETPGNHTWMVWRRNLTEFSSLLFR"
     gene            6045..6263
                     /locus_tag="VGR03_15280"
     CDS             6045..6263
                     /locus_tag="VGR03_15280"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEV1995693.1"
                     /translation="MVYAYDMGRTNIEIDESLVRKARKLTRLKTKREIVDAALELLVR
                     SESRKGILRHYGTGIWKGDLKAMRRNRG"
     gene            6269..6658
                     /locus_tag="VGR03_15285"
     CDS             6269..6658
                     /locus_tag="VGR03_15285"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HEV1995694.1"
                     /translation="MDSSVWIDFFSSAPGRAGDELRRMISDAEPFALTGVVVAEILQG
                     LTRDASRIEHYLSQWEMLEPRGFRTYREAAAIFRLGRAKGVSLTTIDALIAAIVLEHR
                     ASVFTLDKDFSRIARLTGLALYLLPGP"
BASE COUNT         1578 a         1715 c         2147 g         1404 t
ORIGIN      
        1 ggggcatccg gtggcgcggg tttggcgaag cgagagtcat cgatctgcgg atcgatttcg
       61 atttttccgg cggtgagggt ctgcttgata tcggtgcccg gactgccctg ctcgatccgg
      121 aatggatatt tcaggccctg cacttcgtgg aagtccgaga agaaggactc ccagggaagt
      181 gcttggtctc cggtcttctg gatgcctgcc cactttaaaa gcaaaaaaga ggaagcatca
      241 aaaatgtaaa agcgaacgtc cccattcttg ttcgtgagtt tcaggcgaaa gacgggcttg
      301 tcgtccaggt tttctttgcc ggccagttcg atctggttgc ctttggcctt gtagtccacg
      361 agcgggccgt caaagtcgga ttcatcggtg atgttcttaa ggtcttcggg agacagcggc
      421 tgaacgtttt tagcttccgt gaacggattg accgtccagc cggaagactt gccatcgtaa
      481 acgcggataa ttttttggcc ttcaatcgag atttcggcgt gcatcttcag gggacgtttg
      541 agctccacta caaaggtgcc ttccatgccc cctgagaagg agacgtgacc gctgactcgt
      601 tcggattgga cggctttaat cttttcgact ccgccgcgag cgtcgagcgc tttcttgacg
      661 atttcgtcgg cggtttgagc agccgcggcc agcggcaaga acaagagtag agtgatgcag
      721 aggattctgg ttttcatggg cgactcgctg gcggcttgtc tcagtctggg gggtggcgcc
      781 aggttcatcc taggagtaga agcgctttgg gtcaaatgcg ttgccaggcg gggcgtcgcg
      841 tcaggtatat gtgcgctata aacttcgtga aacaattttt tcgtagactc gggtgaatgt
      901 agatttagag ccgcgccgaa ggatagtgca aagaatccac ttcgcggatc ggggaagcag
      961 gcctatgaaa tcgcgagcca taacggttgg ttttcttctg atttgcttgg gtttttgcgg
     1021 aggcgcgtgg tgccagcaag agccggccgg agaggggcgc cccatcacgc cagccggtac
     1081 gctggtcatc gatgcagcga cgcatttgcc ggctgttggg gccatgccca tggcgatgct
     1141 tcgcagtccg gacgctctcg gacacggcgg caagggccgg tatcttctcg tggtcaacag
     1201 tgggtacggt gtccagttca gcgaggacac caatgaagcg cagcaatcga ttgcggtggt
     1261 cgacttgaac gcgactcctg agccgctggt gattcaaaac gtatattttc cttcgccgca
     1321 aagcgcgaac atcggtctgg ctttttcatc tgccgcgggc gccgatggaa actttgaaat
     1381 gtatgtttcc ggcggattcg aaaacaaggt ctggattttc cgttttgatc cgaaagcagc
     1441 gagaccggtc cagccgggat cgaacgggcc agatacgaaa gtagcagcgc cctctttcga
     1501 aatcagcaaa gcgggcgaag tttcgcgctc ggactacaac aaggggaagg cggcactcta
     1561 tccgacgggg ctggcggtaa caccggacgg caccacactg gtgacggcga acaacctggg
     1621 agacagcgtg acgatagtgc gcggcctccg aggggcgcgg cggatggaga gagtggattt
     1681 gcaccatccc ggaaagttga gcgagaacat ttatccgtac ggtgtggtgg tcctgggaag
     1741 cggcaagagc gcgcgggcgt acgtatcgtg ctggaatgat tcgtcggtgg cggtgattcc
     1801 gctgagcggc aaaagcgttg tgcgggagta catcatcgtt gaccggcacc cgacggcaat
     1861 gacgccgaat gccgggggga cgcggctttt tgtatcgaac tccgatgcgg acagcgtttc
     1921 ggtgattgac accacgacgg atcaagagat cgagcgcatt gacgttcggc tggcggagga
     1981 tgccttgccg ggagcgagtc cggaggcggt ggcgctcagc gaagacgaaa agacgctgta
     2041 tgtggcgaat gctcacagca acgcagtcgc ggtggtggcg ctttccgaaa aagcacgcgg
     2101 cgggaaaaca gtgcagaaaa tggctgaagc cgcaaagtcg aaaatcctgg gattcattcc
     2161 gacgggacag tatccatccg cagtcgcttt ggcagacggc aaactcttcg tcggaaatgg
     2221 caaaggaacg ggattcgaat cttcctcgat gcgcgtgaac aatagcggtc gcacgccgaa
     2281 tccacccaac gccgcgtttc ctccgaataa ggaaaagaat ggacagggcg gacagtacag
     2341 cggctcgatt gtttcgggaa acatcagcgt ggttccgctg ccggacggcc ccgctctcgc
     2401 gcattacacg cagcagacca tgcagaatga cgggctgatg gattttgcgc cgcccaagct
     2461 ttttgcgggg aatagcccga tcaaacacgt catttacatc atcaaggaga atcgcaccta
     2521 cgatcaggta tttggcgacg tgaaaacatc gggcgacgga cacgccgcgg atggagaggc
     2581 tgatcttgcg attttcggga atggcgcgac agcgcaacgg cctgatggca cgcggcaagc
     2641 ggtaacgccc aatcatcatg cgctggcgca gcgcttcggt ttgtttgacc gcttcttcgt
     2701 gaattccgag gccagccccg acggacacaa ttggtcgacc gccgcgttct ccaccgatta
     2761 tgtggacaaa ggattccgct gggaatattc cgagcgaggc cgcacgtatg actgggaagg
     2821 atttaaccgg ctgccggatt acgagccacc aagtaatttg caagtcgaaa aattcaaggg
     2881 cgatgtgctg gcggctctga cggatttgct ggaaaaacat cttccctatc accaggggtt
     2941 catggatctc gcggaaccca agagccttta tctatgggat gcggcggcgc gggcgggttt
     3001 gacgtaccgg aactacggag aattcgtaac cgtaatttcg acaaaggatg ttgaagcggc
     3061 ggggcaaaaa cgcaagaaga gctatcccga tgtttcgaat gccgtgcgcg ccatacccaa
     3121 caaagagtcg ctgcaaaagc atcacagccc gagtttccgg agttttgatg tgaccgcgcc
     3181 ggacagcatg accgtggatt gctataaagc agctctggat ctgtcggcct cttccgatcc
     3241 tgcggtaacg cgcgccaatt cgaacgccaa atgccgcggc aattcgcgtt tcggcgaatg
     3301 gctcgccgaa ttcaaagcat tcgctgaaga ccgcgaagcg ggacgcgacg atgcgatgcc
     3361 ggcgctcagc gttctgcgat ttcccaacga tcatacgacc ggaatgaaga atggatttcc
     3421 gacgccgcaa tttatggttg cggacaatga ttacgccgtg ggacggcttg tggaagcggt
     3481 ctcctccagc gtctattgga aagatacggc tatcttcatt gtcgaagatg acgcgcaggc
     3541 cggaccggac cacgtggatt cacaccggtc gctgggcctg gcgatcagcg cttacaatcg
     3601 gccgggagcg ttgatccaca aatttcacag cacggtcagt atgattcgca ccatcgagat
     3661 gctgctggga atcgcgccca tgaaccagct cgatgcgtcg gcgatctcga tggatatctt
     3721 tcaggagacg ccggacctga cgccgtacaa agctgttttg ccaacgattg ctgcagacaa
     3781 cttgatgacg cggaagccga aggacaaaac cactgctgag tggatgaaga aatcgaatcg
     3841 gcaaaacttc gcgcacgcgg acatggccga tccgaaagtg ttgaacgcgg tgatctggtt
     3901 cgcgtgcacg ggaaacggat cgaaccttcc gcaagcggcg cagcttcctg cgtatcaagc
     3961 gatgcgtctg ggaatcgcca aagtcgatga cgacgaaagc gtctcccaga aagacgatga
     4021 cgactgatcc gtcgcggagc tgacaatcac gtttgacggc aacgccgggc gacgacttta
     4081 aggctgcgtt cgggagcgca cgctgggctg ccaagtcaga gtcgcgattc aaaaaccaac
     4141 ctccaagata tcagcggaaa agcagcgagc tgaattccgt caagttgcgg cgccagacca
     4201 tccaggtgtg attgcctggc gtctcgatgt ccacgtgctt gatattcttg gaagcaagcc
     4261 agacacggac cttgcgattg atgtcgatca aacggtcgtc cgtcccgcaa gcaatccaaa
     4321 gcaagtggag ttgctctgtg gctttggagt ccagcgcggg aaattccttg tcaaactctt
     4381 cggtgatacc tccggagctg aatgcgccga tccagccaaa cttgtccagc gtattcaggc
     4441 cggtcagcaa agattcggag ccgcccatgg aaaggcccgc gatggcgcgg gagttgcgat
     4501 cctttttgac gagataagcg gattcgacgc gcggaatgac ttcggtgagc aaagcctcgc
     4561 ggaatttatc gaaatttcga tccgtgagcg cgcgatcgct gaacaccccg gagttgggaa
     4621 gaagaacttc gggcgcgcca tagcccagcg gcatgactac cagcatgggc ttggctttgc
     4681 cttgcgcgat caaattgtcc aggatcacgt tggcgcggcc caccgccgtc caggcgctcg
     4741 cgtcgtcgct gaagccgtgc aacagataga ggacgggata cgtttgcttc ccgcgggggt
     4801 cgtagcccgg cggggtgtaa acgaaatagc cgcgatcgtc gcccacaact cccgacttgt
     4861 aaaaatgatg atgaatctcg ccatggggca ccgcggcgat ctcccaggaa aaagttgccg
     4921 gtccggcgac gtgcaactcg ctcgcccgat agaggaagtt gggcttgatg gcgtgattgg
     4981 aaggatcgaa caggcccacg ccatcggcga tgatcgtgta gccgtaataa tcaggagcga
     5041 gcggatcggt ggttacgctc cagacgccct gatcgtcttt ctgcatgggg aaaggcttgg
     5101 gcacgccttc gatcatgacg gcgacttctt tgtcgttggg agcgcggaag cggaacgtga
     5161 cgcttttatc agcgtggacc tctggagaga tcagcggcgg cggtggcgtt tgcgctggag
     5221 cggtagcggc tgcgagcaag aatgcgaaca acagtaagaa tgtgcgcttc atggtccctc
     5281 gttgtggtgc agctctcctc gaagtcctcg ttcccgagcg ctgcgccgag cgatgataca
     5341 acaagccgcg acggaaagtg gtcagtgaga agcggtcagc ggagagagta cccggggaga
     5401 ggaagcagag agatccatcg gctcttgatt ctcgctcggg atagaaatcc ttcttagagt
     5461 ttctagttaa caataacacc cccctacccc ccgcgttttt tggaagtgcg gattctaaag
     5521 gacttaatga tcgtgtaagc cctttgtttc ccacacttcc gaggggtctc ggatctgcgg
     5581 attctaaagg agataagggc agccggatag ggattggaaa atcggagccc ctccccccta
     5641 tgtttttgta acgatatgat aggacagagt ttaaggggtg ggggtcggca aaggatatga
     5701 tttcatggga gttaggggat aggggacagg gagtagggat taggccagag aaagaaccaa
     5761 gagatcggcg aggatggtgg gtggcaaaca cgcggaacta tatcatgaaa ctaaagctga
     5821 tgtcaagtaa tttgttcgat atcattcgaa tggcgccgta agacgttgaa atgcttgcag
     5881 gttacaggtt tgaaggttga agttcaggga tggagatccg gggcgggaaa gcggaaaatg
     5941 ttgaaaagag agggtcaagg aagaaggtcg aggaaaaatc aaaagcccga ccctcaaaac
     6001 ccgagcgttg gacacctaga taacatacac cgaattctta agaaatggtg tacgcttacg
     6061 acatgggcag aacgaacatt gagattgacg aaagtctggt gcgcaaagcg cgcaagttga
     6121 cacggctgaa gacgaagcga gaaatcgtgg acgcggcgct agaactactg gtgcgctcgg
     6181 aaagccggaa gggcatcctt cggcactacg gaacggggat ctggaagggt gacttgaaag
     6241 cgatgcgaag gaaccgcggt tgatcctggt ggatagctcg gtgtggatcg attttttcag
     6301 ttcagccccg gggcgcgcag gagatgagtt gcggcggatg atcagcgacg ccgagccttt
     6361 tgcgcttacg ggagtggtgg tcgccgaaat ccttcagggc ctcacgcggg atgccagccg
     6421 gatcgagcac tatctttccc agtgggagat gctcgaaccc cgaggcttcc ggacctaccg
     6481 ggaagcggcg gcgattttcc ggctggggcg cgccaaggga gtgagtctga cgaccattga
     6541 tgcactcatc gcggcgattg tgctggagca tcgcgccagc gtgtttacgc tcgacaagga
     6601 tttttctcga atcgcgcgct tgacggggct cgcgctgtat ctgctgccgg gaccatgaag
     6661 cgaagaaagg gcggagttca agagacgggg atcgcggggt gtgaaggcga aaaagtcaaa
     6721 aaccgcaccc tttgaaaaac caaactccaa agggattgat tggataagag cgaaggctcc
     6781 gttcggtagc cgggtatgct tctggttgga ggaccgaagc actcggtagg aaggagcctt
     6841 cgat
//
LOCUS       DASYVO010000444         8631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_179800, whole genome shotgun sequence.
ACCESSION   DASYVO010000444 DASYVO010000000
VERSION     DASYVO010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8631
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179800"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..2077)
                     /locus_tag="VGR03_15290"
     CDS             complement(17..2077)
                     /locus_tag="VGR03_15290"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HEV1995695.1"
                     /translation="MRERKPKRLYTLLFLFLAGLLFWPSTASAQFHPKDTEWPSYAAD
                     LAGTRYRPLDQINAANFSDLEIAWRIKTDNFGDRPEYKLEGTPLMVNGVLYATAGSRR
                     AVIALDPATGELLWIHGEHEGKRGGAAPRQLSGRGLAYWSDGKEERILYVTPGFRLIC
                     LDAKTGVRIPSFGNSGEVDLKLNDDQDIFPDLITGEIGIQSAPVVAKDTVIIGAAFRE
                     GMTPKSMRNNKGYVRGFDVRTGKRLWIFHTIPKKGEFGYDTWEKGSAEYTGNTGVWTQ
                     ITVDEQLGLVYLPVETPTSDFYGGHRPGNNLFGESLVCVDLKTGERKWHFQLVHHPLW
                     DMDISSAPILADITVDGKPVKAVAQPTKQGFLYVFDRVTGKPIWPMPERPVEVGNVPG
                     ETYSPTQPIPTKPAAYSRNGVSIDDLIDFTPALRERAKEIASRYHLGPVYTPPTVSKL
                     DGPLGTLTMGTASGGTNWPGGSYDPETHNVYVYACNSCIEPIGLVPAPKEVSDLNYVA
                     GTAGKEVEILKGPGENAGADSPKPPKKRQGSGYISLNIDGLPMVKPPYGTITAINLDR
                     GEFVWQIAHGETPDVVRNYEALKGLNIPRTGQETYNIGTLVTKTLVIAGEGHVTTTAE
                     HPRGAMLRAYDKKTGQQVGALLMPAIQSGTPMTYMVDGKQYIMVAVSGGNYSGEYIAY
                     SLPN"
     gene            complement(2118..3125)
                     /locus_tag="VGR03_15295"
     CDS             complement(2118..3125)
                     /locus_tag="VGR03_15295"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV1995696.1"
                     /translation="MQTTRRTFLGAVGATAALGVAGGARIPEALAAEPRAANVPAASG
                     EKVLFWVAASTPCDKNLKFDEELYKDLLAYLKANGADGVVVLGTTGEFPSFSVAERKK
                     VAETAFKHRNGLNIIVSPGTANFPETLELSQHAEANGADGLLVVPPFYYKHPRLDGLT
                     RYFSMLFEQMKIPINLYHIPFATGVPISHELLHAMEKYPNLAGIKDSVSDPKVYQDFI
                     AEFPKLNMRTGTTENLKYALDHNMGAILAEGNNFTKQIAAVFTAKRAGKDISETIGKL
                     EEALKLLRTGGVDEYGPMKYALSVQMGTRQFYQRPPNSDVTDEQKAKIKQALEQIKQL
                     G"
     gene            3250..4314
                     /locus_tag="VGR03_15300"
     CDS             3250..4314
                     /locus_tag="VGR03_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995697.1"
                     /translation="MRAMRAFLPFLFSLFFGATVQAQWWQVQTSGIDANLRGVSVARV
                     SNPKGVSALAVWASGSNGVILRSVDKGKTWQRLHVAGGDALDFRGIVAFNASTAYVMS
                     SGEGEKSRIYKTSDGGESWRLQYTDKRKEFFLDTIACLSETHCFALCDPLDGKFLLLN
                     TTDGEHWNPLPSGNMPMALPGEGAFAASNTCLTLSGEEIFFGTGGPAARVFRSPDSGR
                     TWAVTETPIAHGNASSGIFSIARGDEKEIVVVGGDYQDPKRASGVAAYSRDEGKTWQL
                     SEQQPGGYRSAVACDVDAVCVAIGPNGEDASVSRVSAALWKPTDSLNLNAVAILDISN
                     GWAVGPKGTIARFVNHAISY"
     gene            complement(4342..5130)
                     /locus_tag="VGR03_15305"
     CDS             complement(4342..5130)
                     /locus_tag="VGR03_15305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1995698.1"
                     /translation="MNSIPAPLLSLAHVTVMRGDRAALHDISMEIARGEHVCILGPNG
                     CGKSTLIKTITREYYPLAREGSRISILGREQWDIFELRSLLGIVSPDLLASCTTDATG
                     RDVVLSGFFSSTRIFPHHHPDPEHLAGANETLVRLGIAHLAERAVAEMSSGEAKRTLI
                     ARALVHQPQTLLFDEPSNALDIGAQLQLRETMRKLAQSGLGILLVTHHVSEIIPEIER
                     VILLRAGRILADGPKSSVLTKEHLSNLFGVAVRLGQHDGYFHLF"
     gene            complement(5640..8024)
                     /locus_tag="VGR03_15310"
     CDS             complement(5640..8024)
                     /locus_tag="VGR03_15310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV1995699.1"
                     /translation="MKSGTLEEFGTLEITTPLRVLIVEHTRNDVELILLELKEAGLRV
                     NHTVVENREQFRRALQQESFDAILSDYRLPNWTGLDALQELRETGQDIPFLLVTGTLG
                     EEAAVECIKQGATDYILKDRISRLPVALKRAIGEKALRNDAKQTQNALAESETRGRQQ
                     FAELDLLYRSLPVALAVFDCNMRFLRVNDEVSKFDGVPAASHVGLTLREVAPDLANAA
                     EGYLRRVFQTGESISNVELHGGTLQAPDAVRKWLCGFYPLRAEDGTVSTAAVMAIDIT
                     DRKLTQEALDRSEERNRDLVEHSIYGISRVGADGAFLDANPAFLSILGCTTAEELHAL
                     NLMRDVFRFPEQHAELMASCREHGGVHGAESDWRRRDGGIVAVRLHVRMLSIPNHAGA
                     IEIIAEDVTELRAMERQLRQAQKFEAIGQLAGGIAHDFNNVVGAILGWAELGFEQNRA
                     TPQIADRFARIREQAERAAALTRELLAFARRQVLQPRAVDINAVTSGLVSFLDKVIAR
                     DIELKVITAAVDAVKADPTQLEQVLMNLCLNARDAMPSGGRLLIETEMVELDDSYCRF
                     YPYVTPGRYAVLSVSDTGTGMDSETRERIFEPFFTTKANGKGTGMGLATAYGIVKQHG
                     GFIHVYSEPGQGSLFRVYLPAHEGMVAEGSSAKAPAPALVEMRGSETILIAEDHESIR
                     EMARQTLMNLGYRVLSACDGEEALRLCEKEAPSLAILDAIMPKLGGAAAASKLTTLFV
                     GLPILFTSGYSQDSENVAPATADAHYLQKPYSPTTLGRVVREILDQAKKRKATI"
     gene            complement(8021..8500)
                     /locus_tag="VGR03_15315"
     CDS             complement(8021..8500)
                     /locus_tag="VGR03_15315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV1995700.1"
                     /translation="MTSQEYEILLVEDDPADAELTVLSFRKERLANRIEIARDGAEAL
                     DFLFCRGAYAARSFERPPRLVLLDLKLPRVSGHEVLRAIKADERTRFIPVVVLTSSNQ
                     ERDLVECYRLGVNSYIEKPVDFQKFQEVVRQFGMYWLMVNQMPPLAAFTSSNTGGSQ"
BASE COUNT         1682 a         2548 c         2505 g         1896 t
ORIGIN      
        1 cttccaaacg tcagctctaa ttcggcagac tgtatgcgat gtactcgccc gaatagttgc
       61 cgccgctgac ggccaccatg atgtactgct tgccatccac catgtaggtc atcggcgttc
      121 cgctctggat ggccggcatc aacaacgccc cgacttgctg gcccgtcttc ttgtcgtaag
      181 cgcggagcat cgcgccgcgc ggatgttccg ccgttgtcgt cacatgccct tcgcccgcga
      241 tgaccagcgt cttggtcacc agcgtcccga tgttgtaggt ctcctggccg gtgcgcggga
      301 tgttcaagcc cttgagagcc tcgtaattgc gcacgacgtc cggcgtctcg ccatgcgcga
      361 tctgccagac gaactcgcct ctatcgagat tgatcgccgt aattgttccg tacggtggct
      421 tgaccatcgg caatccatca atgttcaaag agatgtagcc gctgccctgc cgttttttcg
      481 gcggctttgg agaatcggcg ccggcgtttt ctcctggacc tttcaggatt tctacttctt
      541 ttcccgcggt tcctgcgacg tagttcagat ccgatacttc tttcggagcc ggtaccagtc
      601 cgattggctc gatgcaggag ttgcaagcat agacataaac gttgtgtgtt tcgggatcgt
      661 aggacccgcc gggccagttc gtgccgccgg aagctgttcc cattgtgagc gtgccgagcg
      721 gaccatccag tttgctcacc gtcggagggg tgtagaccgg gccgaggtga taccttgagg
      781 caatttcttt cgctcgttcg cgaagtgcgg gcgtgaaatc aatcaagtcg tcaatggaaa
      841 ctccattgcg gctgtaggcc gcaggttttg tggggatggg ttgcgtcggt gagtacgttt
      901 cgcccggcac gttgccaact tcgacgggcc gctccggcat gggccagatg ggcttgccgg
      961 tgacgcgatc aaacacgtaa agaaatcctt gcttcgtcgg ctgggccacg gccttcacgg
     1021 gttttccgtc gacggtgatg tccgcaagaa tgggcgccga ggaaatatcc atgtcccaca
     1081 acgggtggtg caccaattgg aaatgccact tgcgctctcc ggttttcaaa tcgacgcaga
     1141 ccaggctctc gccaaacaaa ttgtttcccg gccggtgccc gccgtaaaaa tcgctggtcg
     1201 gtgtctcgac cggcaagtag accaatccga gttgctcatc cacggtgatc tgcgtccaca
     1261 cgcccgtgtt gcccgtatat tccgccgaac ccttttccca cgtgtcgtag ccaaattcgc
     1321 ccttcttcgg gatggtatgg aaaatccaga ggcgcttgcc ggttcgaacg tcgaagccgc
     1381 ggacgtagcc tttattgttg cgcatgctct tcggtgtcat gccttcgcga aaagcagcgc
     1441 cgataatcac ggtgtctttc gccacgaccg gcgctgactg gatgccgatt tcgccggtaa
     1501 tcaaatccgg gaaaatgtcc tgatcgtcat tcagcttcag atcgacttcg ccgctatttc
     1561 caaaggaagg aattcgaacg cccgtcttcg cgtccagaca gatgagccga aatcccggtg
     1621 tcacgtaaag gattcgctct tctttgccat cggaccaata agccagccca cggccggaaa
     1681 gttgccgggg agcggcccca ccgcgttttc cctcatgctc gccatggatc cagagcaatt
     1741 caccagtggc cgggtccagc gcaatcaccg cgcgcctcga tcctgccgtc gcgtataaaa
     1801 cgccgttcac catgagcggc gttccttcga gtttgtactc cggccggtcg ccgaagttgt
     1861 cggtcttgat gcgccaggca atttccaggt cgctgaagtt ggcggcgttg atctggtcga
     1921 gcgggcggta tcgtgttcct gcaagatcgg cggcgtagga cggccactcc gtgtccttcg
     1981 gatgaaactg cgcggatgca gtcgagggcc agaacaggag tcccgcaaga aacaaaaaca
     2041 acaaagtgta cagtcgcttt ggtttccttt cgcgcatctt cgttcctccc ctgtaattcc
     2101 gggccgttgc tttccgctta ccccagctgc ttgatttgtt ccagcgcctg cttgattttt
     2161 gctttttgct catcggtcac gtcggaattt ggtggccgct gataaaactg ccgcgtaccc
     2221 atttgcacgc tgagcgcgta tttcatgggg ccgtactcat cgacgccacc ggtgcgcagc
     2281 aacttcagcg cctcttccag cttgccgatc gtttcgctga tgtccttgcc ggcgcgcttc
     2341 gcggtgaata ccgccgcgat ctgtttcgtg aaattgttgc cttcggcgag gatggcgccc
     2401 atgttgtggt ccagagcgta cttcagattc tccgttgtgc cggtacgcat gttgagcttg
     2461 gggaactccg caatgaaatc ctgataaacc ttggggtcgc tcaccgaatc tttaatcccg
     2521 gcgaggttcg gatatttttc catggcatgc agcaattcat ggctgatggg cacgccggtc
     2581 gcaaacggaa tgtggtagag attgatggga attttcatct gctcgaagag catcgaaaaa
     2641 tatctcgtca atccatccag ccgcgggtgc ttgtaataaa acggcggcac cacgagcaag
     2701 ccgtccgcgc cattggcttc cgcgtgctga gagagctcca gcgtctccgg aaaatttgcg
     2761 gttcccggtg aaacgataat gttcaagccg ttgcggtgct tgaaggcggt ttcggcaact
     2821 ttctttcgtt cagccaccga gaacgatggg aattctccgg tcgttccgag cacgaccacg
     2881 ccatcggcgc cgttggcctt caaataagcg agcaggtcct tgtacagctc ttcatcaaat
     2941 ttcagattct tgtcgcaggg cgtggacgcg gccacccaga acaaaacctt ttctcccgat
     3001 gcggcgggaa catttgctgc ccgcggctcg gcggcaagcg cttcaggaat tcttgcgcca
     3061 ccggccaccc ccagcgcggc ggtcgctcca acggcaccca gaaatgttct tcttgtggtt
     3121 tgcatgattt cctccccggc aggaagcccg cggaagtcta agccagcgga gcgcaagaaa
     3181 caaatgtttc gtttggagtt tgcggcgcgc aggaatcaac aacgcctgtt ggcgccgtcg
     3241 cctgatagaa tgcgcgctat gcgggccttc ctgccatttc tgttttcgtt gttctttggc
     3301 gcaaccgtac aagctcagtg gtggcaagtt caaaccagcg gcatcgatgc caatctgcgc
     3361 ggtgtgagcg tcgcgcgtgt atcgaatccg aagggtgttt ccgcgttggc ggtgtgggct
     3421 tcgggttcga acggcgtgat tctccgctct gtcgacaaag gcaagacctg gcagcggctg
     3481 cacgttgcgg gcggcgatgc gctcgacttt cgcggcatcg tcgcgttcaa cgcttccact
     3541 gcctacgtca tgtccagcgg cgaaggcgaa aaatcccgca tctacaagac aagtgacggc
     3601 ggcgaatcct ggagacttca gtacacggac aaacgcaagg aattcttcct cgacacgatc
     3661 gcctgcctgt cggaaacgca ctgcttcgcg ctctgcgatc ccctcgatgg caaatttctc
     3721 ctgctgaaca ccacggacgg cgaacactgg aatcctctcc ccagcggcaa catgccgatg
     3781 gcactgcccg gcgaaggcgc gtttgccgcc agcaatactt gtcttacgct ctccggcgag
     3841 gaaatttttt tcggcactgg cggtccggcc gcccgcgtat ttcgctctcc ggactcgggg
     3901 cgcacctggg ccgtcacaga aacgccgatc gcccacggca acgcctcctc cggaatcttc
     3961 tccatcgcac gcggggatga aaaggaaatc gttgttgtcg gcggtgacta tcaggatccc
     4021 aagcgcgcat ccggcgtcgc cgcgtattcc cgcgacgagg gcaaaacctg gcaactctcc
     4081 gagcagcaac ccggcggcta tcgctccgca gttgcatgtg acgttgatgc agtgtgtgtg
     4141 gccatcgggc cgaacggcga agacgcgagc gtcagcagag tttccgccgc cctctggaag
     4201 cccacggatt cgctaaatct caacgccgtt gcgatcttgg acatttcgaa tggctgggcc
     4261 gttggaccca aaggcaccat cgcgcgtttc gtaaaccacg caatttcata ctagcgccca
     4321 caaggagaac cgccgccgcg ctcaaaaaag atggaaatag ccgtcgtgtt gtcccagccg
     4381 caccgcgacc ccgaacaaat tagaaagatg ctccttggtg aggacggagg acttcggccc
     4441 gtcggcgagg atgcgtcctg cgcgcaagag aatcacacgt tcgatctctg gaatgatttc
     4501 ggatacgtgg tgggtaacga gcaaaatccc gagcccggat tgcgcaagct tgcgcatcgt
     4561 ctctcgaagt tggagttgcg cgccgatgtc cagcgcgttg ctgggctcat caaaaagcag
     4621 cgtttgcggc tgatgcacca gcgcccgtgc aatcagcgtc cgcttggctt cgcccgagga
     4681 catctcagcg accgcgcgct ccgctaagtg agcgatgccc agccgcacca aagtttcgtt
     4741 ggcgcccgcg agatgctctg gatcgggatg atggtgtgga aaaatgcgcg tgctgctgaa
     4801 aaagcccgag agcaccacgt cacggccggt agcatccgtg gtacaggaag ccagcaggtc
     4861 cggcgagaca atcccgagca gcgagcgcag ctcaaaaata tcccattgct cgcgccccag
     4921 tatggaaatg cgggaccctt cccgcgcgag tggatagtac tcccgcgtaa tggtcttgat
     4981 cagtgtcgat ttgccgcagc cattcggtcc caatatgcac acgtgctccc cgcgcgcgat
     5041 ctccatgctg atgtcatgga gggccgcgcg gtcgccacgc attaccgtga catgcgcaag
     5101 ggagagcagg ggcgccggga tggagttcat tctgtgctca atgcgttgac cgcttcgcgg
     5161 tcacactcag ggatgacagc gtcatcttaa ctgagcgcgg gtttgacgca agggactagg
     5221 gaaggcgaat aagcgaggag gtgactaggc ttgccagcat gagggattgg aagaagattc
     5281 ccatgtctca ttttgaaaca gccgcccgcc cgggcagggc ctaaaaataa aaaggaggtg
     5341 aaaacaggtt cgttctcacc tcccggggtg ggtggggtgt tgcatgacaa taccctcggc
     5401 ggctcagcag gcctgccctg ccaggcaggt ctgcagtctc actcgcgcac tgcacgtggc
     5461 aattcgttag gagactcacc accaaagagc atgtctttct gcggtttgta gcaccgtgga
     5521 atgcacgtgt caaccgtaaa aaaatgaacc gatcgatgag aaaaacgcgc cctttctcca
     5581 gttcactcgg aatcgcgaac gactgcattg cttgctcaat gccgttttga gcgcctgatt
     5641 cagatcgttg cctttcgctt tttcgcctgg tcgaggattt cccgcaccac gcggccgagt
     5701 gtcgtggggc tgtatggttt ttgcaggtag tgtgcgtcgg ccgtggcggg cgctacgttt
     5761 tccgaatcct gcgaataccc gctggtaaaa agaatgggca ggccaacgaa caaggtcgtg
     5821 agcttcgacg ccgcggccgc gccgcctagt ttaggcatga tggcgtccag aatcgcgagc
     5881 gagggcgctt ctttctcaca aagtctcagc gcctcctcgc catcgcaggc cgagagaaca
     5941 cggtaaccga ggttcatgag cgtctggcgc gccatctccc ggatggattc atgatcctcg
     6001 gcgatcagta tggtttccga accgcgcatt tcaacaagag caggagcggg tgctttcgcc
     6061 gatgatcctt ccgcaaccat gccttcgtgt gccggcaggt aaacgcgaaa gagacttccc
     6121 tggccgggct cgctgtaaac gtgaatgaaa cccccgtgct gtttgacgat gccgtaagca
     6181 gtggcgagtc ccatgcccgt gcccttcccg ttcgccttgg ttgtgaagaa cggttcgaag
     6241 atgcgctcgc gggtctcgga gtccatgccc gtgcccgtgt cggaaaccga cagtaccgcg
     6301 tagcggccgg gcgtgacgta gggatagaac cggcagtaag agtcgtccag ctccaccatc
     6361 tcggtctcga tcagcaatcg ccctccgctg ggcatggcgt cccgcgcgtt caagcacaga
     6421 ttcatgagca cctgctcgag ttgcgtcgga tcggccttga ccgcatcgac cgcggcggtg
     6481 ataactttga gctctatatc tctcgcgatt actttgtcga ggaagctaac caggccgctt
     6541 gtaactgcat tgatgtccac ggcccgcggt tgcagcactt gccggcgcgc gaaggccagc
     6601 agttcgcggg taagcgccgc ggctcgttcg gcttgttcgc ggattcgcgc aaatcggtct
     6661 gcaatctggg gagtcgcgcg attctgctcg aaaccgagtt ccgcccatcc gagaatggcg
     6721 cctaccacat tattgaaatc gtgcgcgata cctcccgcaa gctggccgat ggcttcaaat
     6781 ttttgcgcct gtcgcaactg gcgctccatc gcgcgcagct ccgtgacgtc ttcagcgatg
     6841 atttcgatgg ccccggcatg attggggatg gaaagcatgc gcacgtggag acgtactgcc
     6901 acgatgcctc catcgcgccg ccgccagtcg ctttcggcgc cgtgaacccc tccatgttca
     6961 cggcaggaag ccatgagctc ggcgtgctgt tcggggaagc ggaagacgtc tcgcatcaga
     7021 ttgagtgcat gcaactcctc ggcggtagtg cagcccagaa tggagagaaa cgcagggttt
     7081 gcgtccagga aggcgccatc cgcccccacg cgggatatcc cgtagattga gtgttccacg
     7141 aggtcgcggt ttcgctcctc ggatcggtcc agagcttcct gcgtgagttt tcgatctgta
     7201 atgtctatgg ccataaccgc ggctgttgat accgttccgt cctccgcacg caggggatag
     7261 aagccgcaca gccatttccg cacggcgtca ggcgcctgaa gtgtgccgcc atgtagttcg
     7321 acgttcgaga tggattcacc cgtctggaaa accctccgca aataaccttc cgctgcgttc
     7381 gccaagtctg gtgccacctc ccgcagggta agaccgacgt gcgaggcggc gggaacccca
     7441 tcgaatttcg agacttcgtc gttgacgcgc aagaagcgca tgttgcagtc gaaaaccgcc
     7501 aacgcgacag gcagcgagcg atacagcaga tccagttcgg caaattgctg gcggccccgc
     7561 gtttcgctct ccgccaaggc gttttgtgtc tgcttggcgt cattgcggag cgctttttca
     7621 cctatggcgc gcttcagcgc cacgggaagg cgcgagatgc gatctttgag aatgtagtcg
     7681 gtcgcaccct gcttgatgca ctcaaccgcg gcttcctctc ccagcgtccc ggtcaccaga
     7741 agaaacggga tgtcctgtcc ggtctcgcgc aattcctgga gcgcatccag acccgtccag
     7801 ttcggaaggc gatagtcgga gaggatcgca tcgaagcttt cctgctgcag cgcgcggcga
     7861 aactgttccc tattctcgac cactgtgtga ttgacgcgga gtccggcttc cttgagttca
     7921 aggagtatga gctcgacgtc gtttcgcgtg tgttccacaa tgagtacacg cagtggcgtt
     7981 gtgatctcca gagtgccaaa ttcttcgagt gtgcctgatt tcattgcgac cccccggtgt
     8041 tgctggacgt gaaggctgcc aacggcggca tctggttgac catcagccaa tacatgccga
     8101 attgccggac aacttcctga aatttctgga agtcaacagg tttctcgatg tagctgttca
     8161 cgcccagccg gtagcattcc accagatctc gctcttgatt cgaagacgtt agaacaacga
     8221 caggaatgaa gcgtgtgcgc tcgtcggctt tgatcgcgcg gagcacctcg tggccgctga
     8281 cgcgcggaag cttgagatcg agaagtacga gccgcggggg ccgttcgaaa ctgcgcgccg
     8341 cgtatgcccc gcggcagaac agaaaatcga gcgcttcggc gccgtcgcgc gcaatttcga
     8401 tgcggttcgc gagcctctct ttgcgaaaag agagaaccgt gagctcggcg tccgctgggt
     8461 cgtcttcaac aagcaaaatt tcgtattcct gggaagtcat gcgcttactc cgatcacttc
     8521 agggatttgg ctctgcttct tggccttcgc gggaagcgag aaataaaaag tggcgccctg
     8581 atcgacctgg gattccgccc agacgcggcc gccgtgtttg tgcacgatgc g
//
LOCUS       DASYVO010000445         4619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_179865, whole genome shotgun sequence.
ACCESSION   DASYVO010000445 DASYVO010000000
VERSION     DASYVO010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179865"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VGR03_15320"
     CDS             <1..741
                     /locus_tag="VGR03_15320"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV1995701.1"
                     /translation="KELLKKEKEFTRLRDQLSRERRELPWEKVEQKYVFDGPGGKESL
                     GDLFGGKSQLIVYHFMFGPEWKEGCPSCSLLADHFDGARVHLAQRDVTLLAVSRAALP
                     QIEAFKKRMGWRFKWVSSSGSDFNYDYHVSFTKDEMAKREMYYNYGTQAFPSEEGPGA
                     SVFYKNGAGEIFHTYSSFGRGLDIMIGAYNWLDIAPKGRDEDALAFSMAWVRHHDKYD
                     NGYQVDAKAEYVQPAKASDAAGGSCEHH"
     gene            complement(859..1827)
                     /locus_tag="VGR03_15325"
     CDS             complement(859..1827)
                     /locus_tag="VGR03_15325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV1995702.1"
                     /translation="MRSMNKVVTQCAAALVVCTLALPALPQEPPAQGPVIKSQVNLVN
                     LFATVRDKNKRIVPTLKQEDFRIAEDNQDQKIAFFSREVALPITLALLLDTSGSEQDM
                     LSAIQDAGSQFIARIIRKGDEALVMSFDANVDLLSDFTDERSQLERAIHKSRINVPDQ
                     GARVGNPGPIGSRQITGTALYDAIYLACSEKLATEAGRKAIVIVTDAQDEGSKVRLEE
                     AIEAAQRTDTVIHILLVVDPRYGGNAGVAHKLAEETGGRMISVRSEKKLMEAFDEISE
                     ELRSQYTLGYYPTNSAQDGKFRKVKVEMADHDLKVLTRKGYYAPKG"
     gene            1975..3069
                     /locus_tag="VGR03_15330"
     CDS             1975..3069
                     /locus_tag="VGR03_15330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV1995703.1"
                     /translation="MKSLRKTFLLVCGLLLAAALTRLGAQAPQQGTTPAAPEQSPQAP
                     PQENRQGRIIFSVNQVVVPVTVKDGSGKLVADLRRDEFRIFEDNVEQKIVYFGAEPAP
                     ISMVLLIDNDLKSKDAKQVSESLLSVVAGLSLKDEAFVCRFDQFFHEGKGFISDQDKL
                     LTELKRTHLDDQPSTGPSSPAISNGPSINGHSAMGDAPNIAGGLTNIKGQPTKALDDA
                     VYAAAQLLKDRDPERQRRKIIFLISDGVNGPKFNVNKYDTVLKELLRYNIAVYGVGVG
                     SAFFERRFERLSKYAHDTGGDVYYGLKSRAMEELYARVTEEARNQYTLAYAPSGTDRG
                     AEYHSIEVRVTRPGLTILTREGYYAGATPR"
     gene            complement(3045..3398)
                     /locus_tag="VGR03_15335"
     CDS             complement(3045..3398)
                     /locus_tag="VGR03_15335"
                     /inference="COORDINATES: protein motif:HMM:NF022643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HEV1995704.1"
                     /translation="MNRKNIYLFLCILGAAIPYSQFVPWVMENGLHLGLLVRQLFVNR
                     ISAFFGLDVFVSSAVLLVFMRVEGRLLRMRFRWLPIVGLCAVGVSLALPLFLYLRERA
                     LETSKGADYRGVAPA"
     gene            complement(3458..>4619)
                     /locus_tag="VGR03_15340"
     CDS             complement(3458..>4619)
                     /locus_tag="VGR03_15340"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEV1995705.1"
                     /translation="PQVFLLRVNSRAFPATERSRVFEFPVDLMKEGSPAEQFHIQEER
                     RLFYVALTRAEERLTITTVTEKKGKVPVFIEDIVMEPAIKRQDVHQMMPKLPSAGMED
                     RAPEGRTEDAQLFPAASEPAKIFSRIADWAEEFHPPSSEPLTLSPSAVNGYRTCPQQY
                     LFGYLWSLREGPRAAMSFGAVMHTTIKRFVDQLRKGVKLPFDEVRRIFETEWNSKGFE
                     DEYQEGEYKKDGLEQLRVFHAGMMAEPPQALEQEKAFELPMENNVIIKGRIDQINSLG
                     NKRDVEIVDYKTGRPKKDVDAKKDLQLSLYALAVKEILELEPVRLVFHYLQNNQRQET
                     TRDAKQLDEAQKIVQEAAADIRAGEFPAKPGFVCRNCAYKPICPAHEEALSA"
BASE COUNT          959 a         1290 c         1362 g         1008 t
ORIGIN      
        1 aaggagctac tgaagaagga gaaggagttc acgcggctac gcgatcagct gagccgtgaa
       61 cgccgcgaat tgccctggga aaaagtggag cagaagtatg tcttcgatgg gccgggcggg
      121 aaagaatcgc ttggggatct cttcggcggc aagagccagc tgatcgttta ccacttcatg
      181 tttgggccgg agtggaagga gggttgcccg agttgttcgc tgctggccga tcattttgat
      241 ggcgcgcggg tgcacttggc gcaacgcgac gtgacgctgc tggccgtttc gcgggcggcg
      301 ctgccgcaga tcgaagcgtt caagaagcgg atgggctggc ggttcaagtg ggtgtcgtca
      361 tcggggagcg acttcaacta cgactatcat gtgtctttca cgaaggacga aatggcgaaa
      421 cgcgagatgt actacaacta tgggacgcag gcgttcccga gcgaagaagg gccgggcgcc
      481 agcgtgtttt acaaaaacgg cgccggggag attttccaca cttactccag cttcgggcgc
      541 gggctggaca tcatgattgg cgcgtacaac tggctggaca tcgcgcccaa aggccgcgac
      601 gaagacgcgc tggccttttc gatggcgtgg gtgcggcacc acgacaaata tgacaatggg
      661 taccaggtgg acgcgaaggc ggagtacgtg cagcccgcta aggcctcgga cgccgcgggc
      721 ggttcttgcg aacatcattg atcgtgagtc tttctggttg tttagaagat ccagcgggcg
      781 gcttgggcgg caacactcgc gcaccgcgtt cggtgcggga gccttccttt gatgtagccg
      841 cctccgaacc tggaaggctc atcctttggg agcgtagtag cctttgcgcg tgaggacttt
      901 caggtcgtgg tcggccattt ccaccttgac cttgcggaat tttccgtcct gcgccgagtt
      961 tgtcggatag tagcccagcg tatattggct gcgcagctct tcggaaatct catcaaaggc
     1021 ttccatcagc tttttttccg agcgaacgga aatcatgcgc ccgccggttt cctccgccag
     1081 tttgtgcgcc acgcctgcgt tgccgccata gcgcgggtcc accacgagca ggatgtgaat
     1141 cacggtgtca gtgcgctggg ccgcttcgat cgcttcttcc agtcttacct tgctgccctc
     1201 gtcctgtgcg tcggtgacga tgacaatggc tttgcgcccg gcttccgtag cgagtttttc
     1261 actgcacgcc aggtagatcg catcatagag agccgtaccg gtgatttgcc gcgatccgat
     1321 ggggcccgga tttcccactc tagctccctg atccggcacg ttgatcctgc tcttatgaat
     1381 ggcgcgttcc agttggctgc gctcgtctgt gaaatctgac aggagatcga cattggcatc
     1441 gaaggacatc accagcgcct catcaccctt gcggatgatg cgggcgatga attggcttcc
     1501 cgcgtcttga attgcactta gcatgtcctg ctcgctgccg gaagtgtcga gcagcagcgc
     1561 caaggtgatc ggcagggcca cctctctcga aaagaatgcg attttctggt cctgattgtc
     1621 ttcggcaatc ctgaagtctt cctgcttgag ggtgggcacg atccttttgt tcttgtcgcg
     1681 cacggtcgca aacaggttca ccaggtttac ctgcgacttg atcacggggc cttgtgcggg
     1741 gggctcctgc ggcagcgcgg gcagggcgag cgtgcagaca accaatgctg cggcgcactg
     1801 cgtcacaacc ttgttcatgg aacgcatgta tactttcctg tgcgagagat gcccggccgg
     1861 aaaaaaacgc cgcggggtac tgccgcactg taggatgccg cgaagcggtg cgatggatgc
     1921 gtggccgaaa gcacccgcgc ggataatttc agtacgaagt ttggcttgag gactatgaaa
     1981 agtctacgaa aaacctttct gttggtttgt ggattgctac tcgcggcggc actcacacgg
     2041 cttggtgcgc aagcgcccca gcaaggcacg acccctgctg ctccggaaca aagcccgcag
     2101 gcgcctccac aggagaacag gcaaggcagg atcattttct cggtcaacca agttgtggtg
     2161 ccggtcaccg tgaaagacgg gtccggaaag ctggtcgccg acctgcggcg ggacgaattt
     2221 cgaatcttcg aggataacgt cgaacagaaa atcgtttact ttggagctga gcccgcgcct
     2281 atttcgatgg tcttgctgat tgacaacgac ttgaaatcga aggatgccaa gcaagtttcc
     2341 gagagtttgc tgtcggtggt tgcgggcttg agcttgaagg atgaggcctt cgtctgccgg
     2401 tttgaccagt ttttccacga aggcaagggc ttcatcagcg accaggacaa gctcctgacg
     2461 gaacttaaac gcacccatct cgacgaccag ccctcgacgg ggccttcgag tccggccatc
     2521 agcaatggac cgtcgatcaa cgggcactcc gcgatgggcg acgcgccgaa catcgcggga
     2581 ggattgacga acatcaaggg ccagccgacc aaggcgctgg acgatgcggt gtatgccgcg
     2641 gcgcagttgc tgaaggaccg tgatccggag cgacagcggc gcaagattat ttttttgatt
     2701 tcagatggcg tgaacgggcc gaagttcaat gtgaataaat acgacacggt gctgaaggag
     2761 ctgctgcggt acaacatcgc ggtttacggt gtcggcgtgg gcagcgcttt tttcgagcgg
     2821 cgattcgaac ggctttccaa gtacgcgcac gacacgggcg gcgacgtcta ctatgggttg
     2881 aaaagccggg cgatggagga gttgtatgcc agggtcacgg aagaggcccg caaccagtac
     2941 acgttggctt acgctccgag cggaacggac cgcggcgcgg aataccattc catcgaggtt
     3001 cgggtaacgc gcccagggtt gaccatcctg acgcgcgagg gttattacgc aggagcgacg
     3061 ccgcgataat cggctccttt ggatgtttcc aaggcgcgtt cgcgaagata gagaaagagc
     3121 ggcagggcca gactaactcc taccgcgcat agaccgacta tcggaagcca acggaaccgc
     3181 atgcggagca gccttccttc gacgcgcatg aacaccaaga ggactgcgga cgagacgaaa
     3241 acatccagtc cgaagaaggc gctgattcgg ttgacgaaga gttgacggac gagaagacct
     3301 aggtgcaacc cgttttccat gacccacggg acgaattgcg agtaggggat ggcggcgccg
     3361 agaatacaaa ggaacaaata aatatttttg cgattcacag ggtgttctcc agttagactt
     3421 tcgttgagat aatcatagag aatttttcaa gatacgctca ggcgctcagg gcttcttcat
     3481 gcgccgggca gattggcttg taggcgcaat tgcggcagac gaaacccggc ttcgcgggaa
     3541 attctccggc gcgaatgtcg gccgctgctt cttgcacgat tttctgcgcc tcatccaact
     3601 gcttagcgtc ccgcgtggtc tcctgccgtt gattgttctg taagtaatga aacaccagcc
     3661 gcactggctc cagctccaaa atctctttca ccgcaagcgc gtacaagctc agttgcaaat
     3721 ccttctttgc gtccacatcc ttctttggcc gccccgtttt gtaatccacg atttccacat
     3781 cccgcttgtt ccccaaggaa ttgatctggt cgatgcggcc tttgatgatg acattgttct
     3841 ccatcggcaa ttcgaacgct ttctcctgtt ccaacgcctg cggcggttcc gccatcatgc
     3901 cggcatgaaa cacccgcaac tgctccagcc catctttttt gtattcgccc tcctgatatt
     3961 catcttcaaa tcccttcgaa ttccattccg tctcaaaaat cctgcgcact tcgtcaaacg
     4021 gcagcttcac gcctttgcgc agttgatcga caaaccgttt gatggtggtg tgcatcacgg
     4081 cgccaaaact catcgctgcc ctcggtcctt cccgcagcga ccacaaatac ccgaacaaat
     4141 actgttgcgg gcaggtgcgg taaccattca ccgcggaagg gctgagggta agcggctcgg
     4201 aggacggagg atgaaactcc tcagcccaat ccgcaatgcg cgaaaaaatc tttgccggtt
     4261 ccgacgcggc aggaaagagc tgcgcgtctt ccgtacggcc ttccggtgcc cgatcttcca
     4321 ttcccgcgga aggcagtttg ggcatcatct gatgcacgtc ctgccgcttg atcgccggtt
     4381 ccatgacgat gtcttcgatg aaaaccggca cttttccctt tttctccgtc accgtcgtga
     4441 tggtcagccg ttcttccgcg cgcgtcaggg ccacataaaa cagccgccgc tcttcctgaa
     4501 tatgaaactg ctccgcgggg ctgccttctt tcatgaggtc cacggggaat tcgaacacgc
     4561 gggacctctc ggtagccgga aacgcgcggc tattcacccg cagcagaaac acctgtgga
//
LOCUS       DASYVO010000446         2655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_234603, whole genome shotgun sequence.
ACCESSION   DASYVO010000446 DASYVO010000000
VERSION     DASYVO010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234603"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VGR03_15345"
     CDS             <1..188
                     /locus_tag="VGR03_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995706.1"
                     /translation="HIFDWPSSPLEINGLNMKVMSAHLLVSGQSLKFRQAERRLQIDL
                     PQQPPDPNVTVVAVRTL"
     gene            587..1603
                     /locus_tag="VGR03_15350"
     CDS             587..1603
                     /locus_tag="VGR03_15350"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1995707.1"
                     /translation="MATTAPHQQPTPERFFNAINAYEQTEAMKAAVELEIFTAIAEGN
                     TTAATIAKRCEAAERGVRTLCDFLTIHGFLTKEGAQYALAPDSALFLNRHSPAYIGTA
                     IEFLLTPRLREGHARLTEAVRRGGSALGEGTLEPENPDWVKFAQAMMPLMHMPAGIMA
                     AELRKGGEAHKVLDIAAGHGIFGISAAKQNPAAHIYAADWKNVLEVARKNAQAMGVAD
                     RYHLLPGSAFETDFGSGYDLVLITNFLHHFDPPTCTTFMRKVHAALKQGGRAAIAELV
                     PNPDRVTPPTAAAFSLMMLATTPAGDAYTFAELKSISKSAGFARVELAPPEIGLDRLV
                     IAYR"
     gene            2127..2621
                     /locus_tag="VGR03_15355"
     CDS             2127..2621
                     /locus_tag="VGR03_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995708.1"
                     /translation="MLSVRADSTAKHFFLYLVAFFSIAPLAVAGQNNSTNSATKSGSW
                     SGILVSSSCNADEAFNESPECTKNVPGAKLSLYDDTNRVMYGLEPQESVVLHLGDTVT
                     VRGTLDGDTIRMASIESMSIGLAVGQKAPAFSARDQFGRVQTLDSLKGTNGTVLLFFR
                     SADW"
BASE COUNT          567 a          796 c          758 g          534 t
ORIGIN      
        1 tccacatctt cgactggccg tcgtcgccat tggagataaa tgggctgaac atgaaagtga
       61 tgtcggcaca cttgctcgta agcgggcaat cactgaaatt tcggcaggca gagcgtcggc
      121 tccagatcga cctgccacag caaccacccg atccgaacgt aaccgtcgtt gccgtcagaa
      181 ctctttagga gggcgctcgc gatcttgcgc caatgcccga cgtaataccg gtcacccacg
      241 caccaatcgc ggaagcacag tcgatgcgcc gttgccctcc caacttccca gcatcccgtt
      301 cgtggaccgc gccgccattt ctaccgtacg ccgactaccc gtccacagac gtctgagggg
      361 aagcctcaag gcgctggcat agttttcgag tagccgacga tacgtccaga acctcgtgtg
      421 gtactctcca gaaccgcgcg gcccatcttc gttcgcaagt cgcgttgggc gagcccgccg
      481 gagccccgag acgccgttct ccttcggttt ggagccaaga aatatgggat gccgaagaac
      541 agtaattgac ttaaactgct gaggctctga ccgacgggag gaatccatgg ccaccacagc
      601 cccgcatcaa cagcccactc cggaacgctt cttcaacgcc ataaacgcct atgagcagac
      661 cgaggcaatg aaggctgccg ttgagctgga aatcttcacc gccatcgccg aaggcaatac
      721 taccgccgca acgattgcga agcgctgcga ggccgcggaa cgcggcgtgc gcaccctgtg
      781 cgatttctta accattcacg gcttcttgac caaagaagga gcgcagtacg ctctcgcacc
      841 ggattcagct ttattcctca accggcattc gccggcatat atcggcaccg ccattgagtt
      901 tctgctcacc ccgcgcttgc gcgaaggtca cgcacggctg accgaggccg tgcgccgggg
      961 aggctccgcg ctgggcgaag gaacgctgga gcccgaaaat cccgactggg tgaagtttgc
     1021 gcaggccatg atgccgctga tgcatatgcc cgccgggatc atggctgccg aactccgcaa
     1081 aggcggcgag gcacacaaag ttctcgatat cgccgccggt cacggcatct tcggaatctc
     1141 cgcggcaaaa cagaatcccg ccgcacacat ctatgcagcc gattggaaga atgtgctcga
     1201 agtcgcaagg aagaatgcgc aggcaatggg agttgccgat cgctaccacc tgcttcccgg
     1261 cagcgccttc gaaaccgatt ttggcagcgg ctacgatctg gtgttgatca ccaatttcct
     1321 acaccatttc gatccgccca cctgcaccac tttcatgcgc aaggtgcatg ccgcgctaaa
     1381 gcaaggcggc cgcgccgcaa ttgctgaact cgttcccaat cctgatcgcg ttacgccgcc
     1441 caccgcggct gccttcagcc tgatgatgct ggccactacg cccgccggcg acgcttatac
     1501 tttcgcagag cttaagagca tttcgaaaag tgccggcttc gcgcgagttg agctcgcgcc
     1561 gccggagatc ggcctcgatc gcctggtcat tgcgtaccgg tgaattgcgg actgagaggc
     1621 ggcccttgag aggactgcac ctcacccggc tcccttttca tgaggacact gggggagtgc
     1681 acacgaggtt ttgcccccca cccggctgct ccttgggatt gagcctgagt caccgcgcac
     1741 tcctccagaa aaaaaacgcg taggtcgttt gttttctgtc accactgacg gcgcaaaacg
     1801 ccgtccccct gctcggaaat aatccacttg gacgtagaag gcgcttctcg gaaagtgcat
     1861 tgccagctat tgggctgcgt ggtgcgcgat ggttgcgggc agaagcttgg cattgggatt
     1921 taggagacga tggcggagga agaaggcacg gggagcgggg aagcggaaac gtctgggcgg
     1981 aggcgccaca tacgagggtc cattctcggc gtaagtaaag ttgaaaccgt ctcggcgcgt
     2041 caagggtgtc ccacgcggct tgtgcgccga gattgtcaag cgtacagtac gggcggattc
     2101 aaaaatgaaa ggaaggtctc gaattgttgc tgtcggtcag agctgattct acggcgaaac
     2161 acttcttcct ctatctggta gcgttttttt ctatcgcccc tctcgccgtg gctgggcaga
     2221 acaattcaac aaattcggca acaaaatccg gatcgtggtc cggcatcctg gtgtccagca
     2281 gctgcaacgc agacgaagcc tttaacgaat ctcccgagtg tacgaagaac gtgccgggtg
     2341 cgaagctgtc gttgtatgac gatacgaaca gggtcatgta cggccttgag ccgcaggaat
     2401 cggtagttct tcacctggga gacacggtga ccgtccgggg gacactcgat ggcgatacga
     2461 ttcgaatggc ttccatcgag tcgatgtcga tcggactcgc tgttggtcag aaggctcccg
     2521 ctttttcggc tcgcgatcag tttggccggg tacaaactct cgatagcttg aaaggaacga
     2581 acggcaccgt tcttctcttt ttccgttccg cggactggtg accgttctgc aaagggcagc
     2641 tggtccagct gcaaa
//
LOCUS       DASYVO010000447         9129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_207901, whole genome shotgun sequence.
ACCESSION   DASYVO010000447 DASYVO010000000
VERSION     DASYVO010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9129
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207901"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1959)
                     /locus_tag="VGR03_15360"
     CDS             complement(<1..1959)
                     /locus_tag="VGR03_15360"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEV1995709.1"
                     /translation="MKWKMGNRLVLGGFVIATAILVFVGWLSYRNTARFAEASEWRKH
                     TYEVLRNLDETVARLVDAETGQRGYLLTGDDAYLEPYRAAIKSIDQTIGNLKNLTSDN
                     PNQQKRIQILEPLVEKKVTELQRTIDLRKNEGLAAANRVVLEGSGKHWMDQIRAVVAE
                     MANEEEDLLKLRTQKANESVARVVRTILTGTLLSISLLMLCFGLLQRELSERKKAQEA
                     LTKSEKWFSTTLASIGDAVIATDMNGGVTFLNPVAQSLTGWSLEEARGKSMDLVFDIV
                     NKETRRPVENPVKKVFREGKVVGLADHTLLLSKDGKEFDIEDSAAPILTDTGEGFGVV
                     LVFRDITETKRAEEATKRQKELLQLILESIADGVVVADSNGKFLLFNAAAEQVLGIGA
                     TDTTPDQWSDRYGSYLPDAVTLYPSNELPLVRAMRGESVDAVELFIRNAKVPDGRLLS
                     ITGRPLRGEDGALQGGVVVFHDITAQKRAEEALVRAKEEAERANKFKDQFLSTMSHEL
                     RTPLNAILGFSELLTDERYGQMNERQSRYVNHIHTSGKHLLKLISDILDLSKIEAGRM
                     ELTREDVAVASACAEVLSALQPLAGKKSQALLQQVEPSLYVRADATRFKQIIMNLIGN
                     AIKFTPEGGRIELAACRVGEQVRLEVRDN"
     gene            complement(1998..2993)
                     /locus_tag="VGR03_15365"
     CDS             complement(1998..2993)
                     /locus_tag="VGR03_15365"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEV1995710.1"
                     /translation="MSAPQGHLGTILVADDMEANRELLSTLLSAKGYQVVCAADGQQA
                     LERLGSDSIDLVLLDVVMPRRTGFEVCQAMKSKPETRLIPVVLLTSLNSDADRIQGIT
                     CGADDFLSKPVNTHELLARVHSLLRLKQFTDELDNAENVLFSLALSIEAKDPYTEGHC
                     DRLSKYSVALGEKLGLSEELRVALRRGGLVHDIGKLAVPESILLKPGPLTPEERKIME
                     QHVIAGERICAPLRSFRHVLPIIRHHHEKWDGSGYPDGLKGEQIPLTARILQITDIYD
                     ALTTDRPYRKALPVKEALAIMREEVKRGWWDGSILDEFEAVVHGYEPVHPAGTGS"
     gene            complement(3497..4933)
                     /locus_tag="VGR03_15370"
     CDS             complement(3497..4933)
                     /locus_tag="VGR03_15370"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEV1995711.1"
                     /translation="MSGAWKEWEGQTVQGEFPLLKYLGGSSHSAVFLTERAAQEPRKA
                     AMKLVRADPQNAELELSRWARASNLSHPNLIRIFQTGRCQLGGADLLYILMEYADESL
                     SQILPQRPLTPEEVREMLPLILDGLDCVHRHGFVHGRLKPANILATGDQLKLSSDGLC
                     RMGEPRGGREKHTAYDPPEGASGRSTPAGDIWSLGITLVEALTQHLPVSAGTGYAGPL
                     LPETLPAPFLDIVRGCLRRDPQRRFTVADIAVRLKPAQAVPPQQVPPQAAPRRASAKP
                     RLLVPAIAVGLVLLAFVAVPWLLNRPSHPRSDPAVANKQSSPAAPVKSNVQSPSKSKP
                     QPQVQTKPASQPEQKAAARKAREPAPVAASPRTRPETKLRSSGRAQGEVLKKVLPVVA
                     QKSLDTIRGTVRVSVRVHVDPSGEVVGAEFDSPGPSKYFAEQALNAARRWKFDSPLLD
                     GHYVRSEWLLRFHFTPSGVKVIPTQSAP"
     gene            complement(5341..6705)
                     /locus_tag="VGR03_15375"
     CDS             complement(5341..6705)
                     /locus_tag="VGR03_15375"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1995712.1"
                     /translation="MNWGWLLLRIAVAVSLLSLLIASQRFWYRAVWRSSGKLRSVPLR
                     LAIRLLYVAALVAIIVSAADGIRMGHTRLLPHASAIASMAGLWFLSALFAYLAVKAIH
                     ALDHAWRWLRAAGRHKLTPQPPAKPAAAVGASPSFELLLNPSRRYFFQTATALAGATP
                     FLGAVYGFAAERLHYQVHRTEVPVANLPPALDGLRIVQLSDIHLSGYMTREQVRRAVG
                     MANELAPDLAVVTGDFITGASDPLADCVEELRGLRAPLGTWGCNGNHEIYARAEDAAQ
                     LLFAQAGMKLLRQESVRLTWRGARFNLIGVDYQRERTPSGIRLPMLTDLAPLVRRDMP
                     NILLSHNPNSFNRAAELGIELSLAGHTHGGQVQVEILDHRFSPARFFTPYIAGLYHLP
                     FGPAPAPSDSDASSPISNLQTPAANASPRQSCLYVNRGLGTVGPPLRLGVPPEITLLT
                     LRRA"
     gene            6899..7465
                     /locus_tag="VGR03_15380"
     CDS             6899..7465
                     /locus_tag="VGR03_15380"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV1995713.1"
                     /translation="MKKLTLGATAALCLGLLATLLVAQEGAKKVEPPKPAAGPSQALL
                     DQWNDIGRRLIAIAEDMPEDKFDFKPKPEMRSWVQQVLHAAYGNYYFLNPAQGKPLPQ
                     QEQPDGEFKTRAEVVAYVKKVYAEGAELIKSKGDAGMGQTFQMGRRMWRFDALAYDLI
                     AHANEHYGQIVVYYRLNGIVPPESRPRK"
     gene            7485..8087
                     /locus_tag="VGR03_15385"
     CDS             7485..8087
                     /locus_tag="VGR03_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995714.1"
                     /translation="MRKRFAVMAVATLAVLLAGCGPATFLFPLYTEGDKEFDARLLGK
                     WKQVPKPGDKEPDRAHWIFKRNKDDGESYDFSLGSSDPKKGALLSRARLVRLKDYLFI
                     NFETPDLEKRGVTQAPFPAISANFIGRIRIEQDKVRLDMVEDTWIEKEFKAGMLPLSY
                     ISTSQGFVVTAKTEELRKFVVEHAEDDNIFSDSYELTREK"
     gene            complement(8114..>9129)
                     /locus_tag="VGR03_15390"
     CDS             complement(8114..>9129)
                     /locus_tag="VGR03_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995715.1"
                     /translation="HAKNVLWMARDAAEIAKALGREESEIRLTIARGNGKMLAARLPR
                     PTPFVDKTMYGAWNAMFASAYLDAARVFGGSLGESCRAFALKTLDRMLREAWSESRGF
                     RHRIGGPALEGSLDDQAAGVLALLDAYESTLDSGYFRAARFTLDQAIERYGDAEGGGF
                     FDRAADAPPLPGLNVRRKPVQDSPTPGANSLAAIALIRMHAYTDDPRYHDWARKTLEA
                     FAGVASQYGLFAATYGLAATLFLRHPSQVVVTGAARDPAARALEDAANSVFRYGKSVL
                     RLTPGVSYENLPLALKQTLPHLPPDKPAALVCAGQSCLPPTSDPEQLRAMLENSAAGA
                     TSA"
BASE COUNT         1841 a         2572 c         2982 g         1734 t
ORIGIN      
        1 attatcacgc acttccaacc tgacttgctc ccccacccga caggcagcca attcgatccg
       61 gccaccttcg ggagtgaact taatggcatt gccgatcaga ttcatgatta tctgtttgaa
      121 ccgcgtagca tcggcgcgga catacaagct gggctcaacc tgctggagca atgcctgcga
      181 tttcttgccc gccagcggtt ggagggcgct gagcacttcg gcacacgctg acgcgaccgc
      241 cacatcctcg cgagtgagtt ccattctgcc ggcttcaatt ttggaaagat ccaagatgtc
      301 gctgatcagt ttcaacaggt gttttccgct ggtgtggatg tggttgacgt aacgcgactg
      361 gcgctcattc atttggccgt agcgttcatc cgtgagcaat tcggagaatc ccaggatggc
      421 gttgagaggc gtgcgcagct cgtggctcat ggtggagagg aattgatcct tgaacttatt
      481 ggcgcgttcc gcttcttcct tcgctcggac cagggcctcc tcagcgcgct tttgcgcggt
      541 gatgtcgtgg aagacgacga cgccgccctg cagggcgcca tcctcacctc tgagaggtct
      601 gcccgtaata ctgagtaaac ggccgtccgg caccttcgcg ttgcggatga agagctctac
      661 ggcgtcaaca ctctcgcccc gcattgcccg caccaagggc agttcgttgg atggatacag
      721 cgtcacggcg tcaggtaaat agctgccata ccgatcagac cactggtcgg gtgtcgtgtc
      781 cgtcgcaccg atgcccagga cctgctccgc ggccgcattg aagaggagaa acttcccatt
      841 ggaatcggcc acaaccacgc catcagcaat actttccaga atcaattgca ggagttcttt
      901 ttgccgtttt gtcgcctctt ctgcacgctt cgtttcggtg atgtcacgga aaacgagcac
      961 aaccccgaat ccttctcctg tatccgtcag aatgggcgcc gcgctgtctt caatatcaaa
     1021 ttccttgccg tccttcgaga gcaaaagcgt atggtcggcc agaccgacga ccttcccctc
     1081 gcggaagact ttcttgacag ggttctcgac ggggcggcga gtttccttgt tcacgatgtc
     1141 aaacaccaga tccatggact tcccgcgagc ttcctccagg ctccaccctg tcagggactg
     1201 ggcgacggga ttcaagaatg tcacgccgcc gttcatgtct gtggcgatca ccgcgtcacc
     1261 aatgctcgcg agcgtggtcg agaaccactt ttcacttttt gtcagtgctt cttgcgcctt
     1321 tttacgttcg gacagctcgc gttgcagaag cccgaaacac aacatcagca acgaaatgct
     1381 cagtagggtg ccggtgagga tggtgcgcac caccctggcc acgctctcgt tggccttttg
     1441 tgtgcgaagc tttaggagat cctcctcttc gttcgccatt tctgcaacca cggcgcggat
     1501 ctggtccatc cagtgctttc cggaaccctc cagcaccacc ctgtttgcgg cagccaggcc
     1561 ttcattcttg cggaggtcga tcgtccgttg aagctccgtc actttttttt ctaccagagg
     1621 ctcgaggatc tggatgcgtt tctgctgatt cggattgtcg gatgtgagat tcttcaggtt
     1681 cccaattgtc tggtcgatgc tcttaatcgc cgcacggtac ggctcgagat aggcatcgtc
     1741 ccccgtgagg agatagcctc gctgtccggt ttccgcgtca acgagccggg ccaccgtctc
     1801 atcaagattc ctgagaacct cgtaggtgtg cttccgccac tcggaagctt cggcgaatcg
     1861 ggccgtattc cggtaggata gccatccaac aaacaccaga atggccgttg ctattacgaa
     1921 tcccccaagg accaggcgat ttcccatttt ccatttcatc tttccaccgc cttgccacgg
     1981 agtctcggga gcaggcatta gctaccggtc cctgccggat gcaccggctc atagccatga
     2041 acgacggcct cgaattcgtc caggatggac ccatcccacc atccgcgctt cacttcctcg
     2101 cgcatgatgg caagggcttc tttcacggga agagccttgc ggtaggggcg gtccgtggta
     2161 agcgcgtcgt agatgtccgt gatttgaagg atgcgcgcgg taagtggaat ttgttcgcct
     2221 ttcagcccgt cgggataccc cgagccgtcc cacttttcat ggtggtggcg aatgatcggc
     2281 agcacatgac ggaaggaccg gagcggggcg caaattcgct cgcccgcgat gacgtgctgc
     2341 tccatgattt ttcgttcttc cggagtcagc ggtccgggtt tgagcaggat gctctcgggc
     2401 acggccagct tgccgatatc gtgcacgagg ccgccccggc gcagagcaac gcgcagttcc
     2461 tcggataaac ccagcttttc cccgagcgcg acggaatact tcgaaagacg atcgcagtgc
     2521 ccttcggtat aggggtcctt cgcctcaata ctcagggcga ggctgaacag tacgttctcg
     2581 gcgttatcca gttcatcggt gaattgcttc aagcggagca gggagtgcac acgggccagc
     2641 agctcgtgcg tgttcacggg cttgctgagg aaatcatcag cgccacacgt gatcccctgg
     2701 atacggtcgg cgtcgctgtt caaactggtg agcaatacga cggggatcaa gcgggtttcc
     2761 ggcttggact tcatcgcctg acaaacttca aagccggttc ggcgcggcat cacaacgtca
     2821 agcaagacca aatcaatcga gtcgctaccc aggcgctcta atgcttgctg gccgtctgct
     2881 gcgcagacga cctggtaacc ctttgcgctc agcagagtgg agagcagttc gcggttagct
     2941 tccatgtcgt cggctacaag gatggttccc aggtggcctt gcggagcaga catatcccct
     3001 ctttaacgat tagctcgacc gcacaagaag gttgcgagcc tcaactctcg tcgagagatg
     3061 gaagagggaa ttcatccagt cccacgcaac gaagaggtat cgcatcagac ctaattagaa
     3121 gtatcccaaa cctggacggt cttgtgttta cttcccagga gtacggagtc aagtagtaaa
     3181 taaggcgacc gaaagattcg gaatgtctcc agcaggttct cctcaatggc cccggaaagt
     3241 cggcttccct tcaactgagc tgcacatacc tggccgtata acgcggttga cggaagttga
     3301 cccgcaggtg tgctttcccg aaagttccct gacagagcca tggtggcctc agcggccctt
     3361 cgttcctcag ggcaagctag agcccggttt gttttctaca cttgatgtcg gagctgaagc
     3421 tccgacccct gaacgccgcc ggagtcgagc caaaacccac gcttctcgcc gggaagcctg
     3481 gggcaccggc gagcggttaa ggcgcggact gcgtggggat gactttgacg ccggaggggg
     3541 tgaaatgaaa gcggaggagc cattcgctgc ggacgtagtg gccgtcgagc aggggcgagt
     3601 caaatttcca gcggcgcgcg gcgttcaagg cctgctcggc gaagtatttg ctcgggccgg
     3661 gtgaatcgaa ttcggctccc acgacttcgc cggacggatc cacatggact cgcacgctga
     3721 cccgtaccgt cccacgaatg gtgtccagtg atttctgggc tacaacgggc agaacttttt
     3781 tgaggacttc accctgagcg cggccgctgg agcgcagctt tgtttccggc cgcgttcgcg
     3841 gagaggccgc cactggcgcg ggttccctgg ctttgcgtgc cgcagccttc tgctcgggct
     3901 gtgacgcggg cttcgtttgg acctgcggct gcggcttgga tttcgaaggg gactgaacat
     3961 tggactttac cggggccgca ggagaggatt gcttgttggc aacagccgga tccgaccgag
     4021 ggtgcgaagg gcggttgagg agccacggga ctgcaacgaa cgcgagaagt acgagaccca
     4081 ctgcgattgc aggcacgagg aggcggggtt tcgcggaggc cctccgcggc gccgcttgag
     4141 gaggaacctg ctgcggtggc acagcctgag ccggcttaag ccgaaccgcg atgtcagcga
     4201 cggtgaaccg gcgctgcggg tctcgccgca ggcagccccg cacaatgtcg agaaaaggcg
     4261 caggcaacgt ctccggcagc aatggccctg cgtatccggt tcccgccgaa acgggcagat
     4321 gctgcgtcaa ggcttcgacc agcgtgatgc caagcgacca gatatccccg gccggagtgc
     4381 tccttccgct tgcaccctcc ggcgggtcgt aggcggtgtg cttctcccgg ccgccgcgcg
     4441 gctcgcccat ccggcagagc ccgtcactcg agagcttcaa ttgatcgccc gtcgccagaa
     4501 tgttcgcggg tttcaaacgg ccatgcacaa aaccgtggcg gtgaacgcag tccagcccgt
     4561 ccagaatcag agggagcatc tcgcggactt cctcgggtgt gagcggccgc tggggaagaa
     4621 tctgcgagag gctctcatcg gcgtactcca tcaagatgta gagcagatcc gcgcccccca
     4681 actggcagcg tccggtttgg aaaatccgga tcagattggg atgcgataaa ttcgaagccc
     4741 gagcccaccg ggagagttcg agttccgcgt tctgcggatc agcgcggacg agcttcatcg
     4801 cggcttttcg tggttcctgc gcagcgcgct cggtgaggaa gacggcgctg tgactggagc
     4861 cacccaggta cttcaggagg gggaactcac cctggacggt ttgtccctcc cactctttcc
     4921 aggccccgct cattttggcc tccccctgtc cgcaggcaga cgtcaacgat gtggcatacg
     4981 agcggcacgc ggcgggcgaa accggagttg gagcgtccca gcgtccggag caggatggat
     5041 tccttatata ccggctgaca ggcgcgggat tctcggaact ggtccgcaga gcttcgctat
     5101 tctaggctgg gttcgtgccg acgacaacag gcggccttcc cccgtaccag aggcagcggc
     5161 ggaaagacct tccccactat agtgagctgg ttgccgcgaa aaagtaagtg ggagcttgtc
     5221 caaaaacaca tggcggcgcg ccgcagggag agttgatgcg tctatgtgta cgtcgaagca
     5281 agccgccatc gccctgagag gacggtcggt gcggtttagc tccaaaaagt tggaacggca
     5341 ctaagcgcgg cggagagtga ggagggtgat ctccggggga acgccgagac gcaagggagg
     5401 tccgacggtg ccgaggccgc ggtttacgta gaggcaagat tggcgaggtg aagcgttagc
     5461 agcgggggtt tggagattag agatgggtga agaagcatca gagtcggagg gagcaggagc
     5521 cggtccgaag ggaagatggt acaggccggc gatgtagggg gtgaagaagc gcgcggggct
     5581 gaaacgatgg tcgaggattt cgacctgaac ctgcccgccg tgggtgtgac cggcgagaga
     5641 gagctcgatg ccgagctcgg cggcgcggtt gaaggagttg ggattgtgcg agagaaggat
     5701 attgggcatg tcgcggcgga caagcggcgc caggtccgtc agcatgggga ggcggatgcc
     5761 gctgggggtg cgctcgcgct ggtagtccac gccgatgaga ttgaaccgcg cgccgcgcca
     5821 agtgagccgc acactctcct gccgcaggag cttcatgccc gcctgggcaa agagaagctg
     5881 cgcggcgtct tcggcccggg cgtagatttc gtggttgccg ttgcagcccc aagtgccaag
     5941 tggggcgcgc agcccgcgga gctcttcgac gcagtcagcc aggggatcgg aggcgccggt
     6001 gatgaaatcg ccggtgacga cagcgaggtc gggcgccagc tcattggcca tgcccacggc
     6061 acggcggacc tgttcccggg tcatatagcc gctgagatga atgtcactga gctggacgat
     6121 gcgcaggccg tccagagccg gcgggagatt ggcgacggga acctcggtgc gatggacctg
     6181 gtagtgcagg cgttccgcgg caaagccata gactgcgccg aggaatggcg ttgcgcccgc
     6241 cagcgcggtg gccgtctgga agaagtaccg gcgtgaggga ttgagcagga gctcgaagga
     6301 gggggaggct cccacggcgg ccgcgggctt cgccggcggc tggggcgtca gcttgtgccg
     6361 cccagcggcg cgcagccagc gccaggcgtg atcaagcgcg tgaatcgcct tgacggcgag
     6421 ataggcgaag agcgcgctca agaaccacag tccggccatt gatgctatcg cggaagcgtg
     6481 gggcagcaga cgcgtatggc ccatgcggat gccgtccgcg gccgagacaa tgatggcgac
     6541 aagcgcagct acgtagagaa gccggattgc aagacgcagg ggaacgcttc gaagcttgcc
     6601 ggaagaccgc cagacggcgc ggtaccagaa acgctgcgag gcaatgagca gcgaaaggag
     6661 actcacagcg acagcaatgc gaagaagaag ccagccccag ttcacggttt gttctgctcc
     6721 cttagcgtaa aagactagtt tagcatgccg gggcggggct tttcgaaagt gacctgcgga
     6781 agggttccca agtaagatag tggcctcggg accgcggaca gggcttctct ccatggataa
     6841 cattcaaggt gaggtatccg tctaagggac cgaggagagc tggacggagg gagaaaatat
     6901 gaaaaaactg actttggggg ccactgcggc actgtgcctg gggctgttgg cgacgctgct
     6961 ggtggcgcag gagggggcaa agaaagtcga gccaccgaaa ccggcggccg ggccatcgca
     7021 agcactgctg gaccagtgga acgatatcgg gcggcggctg atcgcgattg cggaggatat
     7081 gccggaggac aagttcgatt tcaagccgaa gccggagatg cggagctggg tgcaacaggt
     7141 gctgcacgcg gcgtatggaa actactactt cctcaatccg gcacagggga agccgttgcc
     7201 gcagcaggaa cagccggatg gggagttcaa gacgagggcg gaagtggtgg cgtacgtgaa
     7261 gaaggtgtac gcggaagggg cggaactgat caagtcgaag ggcgatgccg gcatggggca
     7321 gaccttccaa atggggcgga ggatgtggcg gttcgatgcg ctggcctacg atctgatcgc
     7381 gcacgcgaac gagcactacg gccagatcgt ggtgtactac cggctgaacg gaatcgtacc
     7441 gcccgagtcg cggccgagga agtagcggcc cgcagggagg aatcatgcgg aagaggttcg
     7501 cggttatggc ggtggcgacg ctggcagttc tgctggcagg ctgcgggccg gccaccttcc
     7561 ttttcccgct ttacacagag ggtgacaagg agtttgacgc gcgcctgtta ggaaaatgga
     7621 agcaagtgcc caagccgggc gataaagagc cggatcgtgc gcactggatc tttaagcgca
     7681 acaaggatga cggtgaaagc tacgacttca gccttgggag cagcgaccca aagaagggag
     7741 ccttgctcag ccgggcgcgt cttgtccggc tgaaggatta tctcttcatt aattttgaga
     7801 cgcccgatct ggaaaaacgc ggagtgacgc aagctccctt ccctgcgatc agcgcgaact
     7861 tcataggcag aatccgaatc gaacaagaca aggtacgcct ggacatggtg gaagacacct
     7921 ggatcgagaa ggagttcaag gcgggaatgt tgccgctctc ctacatctcc acgtcacaag
     7981 ggttcgtggt caccgcaaag accgaagagt tgagaaaatt cgtcgtcgag catgcagaag
     8041 acgataacat tttttcggac tcctacgaac taacacgcga aaaataggga agccgtggcg
     8101 cggcacggaa gcgtcaggcg gaggttgcgc cagcggcgga gttctcgagc atggctcgga
     8161 gttgctcagg atcactggtc ggcgggagac aagactgtcc cgcgcaaacg agggcggcgg
     8221 gtttgtccgg cgggagatga ggcagagtct gcttgagggc gagcggaaga ttctcgtacg
     8281 aaacgccggg agtcagtcga agcacggact tgccgtaacg gaaaacgctg ttggcggcgt
     8341 cctccaatgc gcgggcggcg ggatctcttg ccgcgcctgt gacgacaacc tgcgacggat
     8401 gacgaaggaa aagagtcgcc gcgaggccgt aggtggcggc gaagaggccg tactgtgaag
     8461 cgacgccggc aaaggcttcg agagttttcc gcgcccaatc gtgatagcgc gggtcatcgg
     8521 tataggcgtg catgcggatg agggcgatgg cggcaaggga gtttgcgccg ggcgtgggcg
     8581 aatcttgaac gggcttgcgg cggacattga ggcccggcag cggcggagcg tcagcggcac
     8641 ggtcaaagaa gccgccgcct tcggcgtcgc cgtagcgctc gatggcctga tcgagtgtga
     8701 agcgggccgc gcggaagtag cccgaatcaa gagtggattc ataggcgtcg agcagggcga
     8761 ggacgccggc ggcctgatcg tccagcgatc cctccagcgc gggccctccg atgcggtggc
     8821 ggaaaccgcg ggattcggac caggcttcgc ggagcatgcg gtcgagcgtt ttcaaggcga
     8881 aggcgcggca gctttcgccg aggctccctc cgaaaacgcg cgccgcgtca aggtaggcag
     8941 aggcgaacat ggcgttccag gcgccgtaca tggtcttgtc cacgaagggc gtggggcgcg
     9001 gcaggcgcgc cgcgagcatc tttccgttcc cgcgggcaat cgtcagccgg atttctgatt
     9061 cttcccggcc gagcgccttg gcaatttcgg ccgcgtcgcg cgccatccac aagacgtttt
     9121 tcgcgtgat
//
LOCUS       DASYVO010000448         5768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_169110, whole genome shotgun sequence.
ACCESSION   DASYVO010000448 DASYVO010000000
VERSION     DASYVO010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169110"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VGR03_15395"
     CDS             complement(<1..506)
                     /locus_tag="VGR03_15395"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV1995716.1"
                     /translation="MRIETSSGRAPGRIVLAASIGLVIIIWAINFIAAKIGLRSLPAA
                     TLASFRVVLAGAVMIPFYLFCSRLPAFAGATETRRRDFTPRDLWTFLYMGFFGVTINQ
                     VCFTVGLRYTSVSHAAVIVGMGPIYTLIFAVLFKLETATWRKAIGMTIAFAGIAVMAS
                     ENGISTHSP"
     gene            complement(511..1617)
                     /gene="menC"
                     /locus_tag="VGR03_15400"
     CDS             complement(511..1617)
                     /gene="menC"
                     /locus_tag="VGR03_15400"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEV1995717.1"
                     /translation="MRIREITLQEVRMKLVTPFETSMDRTDVRRFLLVQADVDGVIGW
                     AECVAGETPFYSPETTDTAWLIMRDFLWPLIKGKDFASAGEVWDLLARVRGHNMAKAA
                     LEAAVWDAEAKQKSVPLSKLIGGVHEEIACGVSIGIKESLDELVAAVQKELAAGYQRI
                     KIKIKPGKDLEPVRRLRQDFPRIKLMVDANSAYTLNDWPLLKQLEGFYLMMIEQPLGW
                     DDLYSHVELQKKLDTPICLDECIHTEEQARAAIVLGACKIINIKLGRVGGHTVARRIH
                     DLCQQHSIPVWCGGMLESGIGRAHNIALSTLPNFTLAGDVAASKRYWEEDIILPEVTV
                     SPRGTIRVPTGPGIGFEPRLDRIDKLTVRKERLA"
     gene            complement(1642..2382)
                     /locus_tag="VGR03_15405"
     CDS             complement(1642..2382)
                     /locus_tag="VGR03_15405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1995718.1"
                     /translation="MSPEIVVRKCESLDDFHRCVDLQREIWGEADLEVEPATMFVVAA
                     HTGGQVLGAFDGDHLVGYTLAVVGLRDRVPYLHSHTTGVRGEYRNRGVGRMLKLFQRT
                     EGLGRGIRLIQWTFDPLELRNAHFNLNRLGAICRKYQPNLYGVTTSPLHRGLATDRLL
                     VEWHLDSARVVAAIENLVKDPGEAPAIIELPAELERWQSEGSSQVGVVQAHVREEFTK
                     WFAKGYAAVALRTAPGNPAYLLTPWSDF"
     gene            complement(2379..3344)
                     /gene="mazG"
                     /locus_tag="VGR03_15410"
     CDS             complement(2379..3344)
                     /gene="mazG"
                     /locus_tag="VGR03_15410"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEV1995719.1"
                     /translation="MKSKTSRMVIRAKQREKKKATLRRDRKPEKQKQLTPGEWFEKLV
                     AVQARLRAPNGCPWDREQTHQSLRTYLIEEAYEVLEALESGNDAKFAEEMGDLLLQIV
                     FHSQIAREEGRFTVAEVIREIHNKMIRRHPHVFGKTRAKDSAEVLRNWEQIKAEERRT
                     GDNKGDSKTGDGAPKEFSLLDGVSRALPATLEGFQLTRKASRIGFDWDDAGGVFEKMQ
                     EETEELKKAMGEQDHLKMEEELGDLLFAAVNLSRFLKIDPEIALKKANAKFSRRFRAM
                     ERLARENGREFKDFPREQMEAFWNAAKKSEGKRHLPEMSGAHTKQ"
     gene            complement(3882..4115)
                     /locus_tag="VGR03_15415"
     CDS             complement(3882..4115)
                     /locus_tag="VGR03_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995720.1"
                     /translation="MRGFGLFCCAIVTTSLFCAPVGQAQNVAAPSSPSEAAAGQGGCE
                     VYIALQPIEPTAAARLAALIVPVRRIWRASGMN"
     gene            4340..4672
                     /locus_tag="VGR03_15420"
     CDS             4340..4672
                     /locus_tag="VGR03_15420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1995721.1"
                     /translation="MSPEPTSLLQGTLDLLILKSLIAGEMHGLGISRRIQQITGGTFV
                     VKPGSLFPALHRMEEEGWISSFWGDSENNRRAKYYRLTKPGRNQLDVETKRWGRISWA
                     IAQALEAS"
     gene            4686..>5768
                     /locus_tag="VGR03_15425"
     CDS             4686..>5768
                     /locus_tag="VGR03_15425"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1995722.1"
                     /translation="MPLLVKVRSFLRNLFLSRRVEVDLDQEVHSHLEMLIAENIRAGM
                     PPHEAQRAARIELGGIEQVKEQVREERIGNWLHSVYSDCRYGVRQLRKNLAATVIMVF
                     TLALAIGATTAIFSVVYGVLLRPLPYPDANRIMAVFEVTSKGRPSRVADPNFDDFRDQ
                     SRSFQAIAKYNENVASVSGASQPTRTTVADVSPDFLRVFGIQPILGRDFSASDAKKGA
                     GPTVFVSYGYWRQHLGSSPDLSQSHLKIDGGVFSVIGVLPAGFRFPADVDLWLPADLD
                     GENPSRTSHNYNAVGRLRNGVTVEQANRDISAIARRIHDTSSEQGDYLLKDGIVVPLQ
                     DSITGRARSALLVLLGAVGFLLLVACA"
BASE COUNT         1146 a         1691 c         1649 g         1282 t
ORIGIN      
        1 ggtgaatgcg tggaaatgcc gttttccgat gccatcacgg caatccccgc gaacgcaatg
       61 gtcatgccta tcgcttttcg ccatgtggcc gtttccaatt tgaagagtac tgcgaagatc
      121 agcgtataga tcggccccat ccccacgatc acggcggcat ggctgacaga ggtgtatcgc
      181 agtccgaccg tgaagcaaac ctgatttatc gttacgccga agaagcccat gtacaggaag
      241 gtccagagat cgcgcggggt aaagtcgcgc cgccgagttt cggtcgctcc ggcaaaggcg
      301 ggcagccgcg agcagaacaa atagaaaggg atcatcaccg cgcccgcaag cacgacgcgg
      361 aacgatgcca gcgttgcggc aggcagagac cgtagcccga ttttcgcggc gataaagttg
      421 atcgcccaaa tgatgatgac gaggccgatg gatgcggcaa ggacgattcg gcctggtgct
      481 cttccgctgg aggtctcgat tctcacagcg tcaggccagg cgttcttttc gcacggtaag
      541 tttgtcaatg cggtccaagc gcggttcaaa accgatgccc gggcccgtgg gcacacgaat
      601 cgtgcctcgt ggcgacacgg tgacctcagg aaggatgatg tcttcctccc aatagcgttt
      661 gctggcggcc acatcgccgg caagggtgaa attaggaagc gtcgagagcg cgatattgtg
      721 ggcgcggccg atgccggatt cgagcatccc cccgcaccag acgggaatgc tgtgctgctg
      781 gcaaagatcg tggatgcgcc gcgccacagt gtggccgccg acgcggccca gcttgatgtt
      841 gatgattttg caggcgccca gcacgatggc ggcgcgcgcc tgctcttccg tgtgaatgca
      901 ctcgtccagg cagatcggcg tgtccagctt tttctgcagc tcgacgtggc tatagagatc
      961 gtcccagccg agcggctgtt cgatcatcat caggtagaag ccttcaagtt gtttgaggag
     1021 cggccagtca ttcagcgtgt aggcggagtt ggcgtcgacc atgagtttga tgcgaggaaa
     1081 gtcttggcgc aggcggcgca ccggttccag gtcctttccc ggcttgattt ttatcttgat
     1141 gcgctgatat ccggcagcga gctctttttg cacggccgcc acgagttcgt ccagtgattc
     1201 cttaatgccg atggaaacac cgcaagcgat ctcctcgtga acgccgccga tgagttttga
     1261 aagaggcacg ctcttttgtt tggcttcggc atcccagacg gctgcttcca gtgcagcctt
     1321 ggccatgttg tggccgcgga cacgcgcgag taagtcccac acttcgccgg cggacgcgaa
     1381 atcctttccc ttgatcagcg gccaaagaaa atcgcgcatg atgagccacg cggtatcggt
     1441 cgtttccgga ctgtaaaacg gcgtctcgcc cgcgacgcat tcggcccagc cgatgacccc
     1501 gtctacatct gcctgtacca gaagaaagcg ccgaacgtcg gtgcggtcca tgctggtttc
     1561 gaatggggtc accagtttca tccgtacttc ctgcagtgtg atctctcgga ttcgcatggt
     1621 ttttcggctg atgtaccttt cctaaaagtc gctccaggga gtgaggagat acgccgggtt
     1681 tcccggtgcg gtgcgtaagg cgaccgcggc gtagcctttg gcaaaccact tcgtaaactc
     1741 ttcgcggacg tgggcttgga cgacgccgac ctgcgaggaa ccttcgcttt gccagcgctc
     1801 aagctccgcg ggcaattcga tgatcgccgg cgcctcgcca ggatctttga cgagattttc
     1861 aatagcggcc acgacgcgcg ccgaatcgag atgccattcg acgagcagcc gatcggtcgc
     1921 caggcctcgg tgcagcggac tggtcgtaac accgtacaaa ttgggctgat attttcggca
     1981 aatagcgcca agccggttca agttgaagtg cgcgttgcga agttcgaggg gatcgaaggt
     2041 ccattgaatg agccggattc cgcggcccag gccttcggtg cgctgaaaga gcttcagcat
     2101 gcggcccacg ccgcggttgc ggtactcgcc gcgcaccccg gtcgtgtgag aatgaaggta
     2161 aggtacgcgg tcacgcaggc caaccaccgc gagcgtgtag cccaccaggt ggtcgccatc
     2221 gaaagcgccc agcacttggc cgccggtatg cgcggcgacg acaaacatgg tagccggctc
     2281 aacttccagg tcggcttcac cccaaatctc gcgctggaga tccacacaac ggtggaagtc
     2341 atcgagactt tcgcacttcc ggacgacgat ttccgggctc attgtttcgt atgtgctccg
     2401 ctcatttccg gcaagtgccg cttcccttcc gacttcttcg cggcgttcca gaaggcctcc
     2461 atctgttcgc gcggaaagtc tttgaactcc cggccatttt cccgggccaa ccgctccatg
     2521 gcgcggaatc ggcgcgagaa ttttgcattg gccttcttga gggcgatttc cgggtcgatt
     2581 tttaagaagc gcgagaggtt tacagcggcg aagagcaagt cgcccagctc ctcttccatt
     2641 ttcagatggt cttgctcccc cattgctttc ttcagctctt ccgtttcttc ctgcattttc
     2701 tcgaaaacgc cgccggcatc gtcccagtca aagccgatgc gggaagcctt gcgagtgagt
     2761 tgaaaccctt caagagttgc gggcaaggcg cgggagacgc catccagcaa ggagaattcc
     2821 ttcggggcgc catcgccggt ctttgaatca cctttgttgt cgccagtgcg ccgctcttcg
     2881 gccttgatct gttcccagtt tctcaacacc tcggcggaat ctttcgcgcg cgtcttcccg
     2941 aaaacatggg gatgccgccg gatcatcttg ttgtggattt cgcgaatcac ctcggccacg
     3001 gtgaagcggc cttcttcgcg ggcgatctgc gagtgaaaaa caatctgcaa aagcaaatcc
     3061 cccatctctt ccgcaaactt ggcgtcgttc ccgctttcga gggcttccag aacttcgtag
     3121 gcttcttcga tgaggtaggt tcgcagcgat tgatgcgttt gctcgcggtc ccaagggcat
     3181 ccgttcgggg cgcgcaatct ggcctgaacg gcgacgagct tttcgaacca ttcgccgggc
     3241 gtcaattgtt tctgtttctc cggcttgcgg tcacgacgaa gtgttgcttt tttcttctcc
     3301 cgctgcttcg cgcgtataac catccggctc gtctttgatt tcacttcaag cctgtcttgc
     3361 tcaaatattt gagcatcgta gtgaccgtgg attgagagat gccgcctccg gcgtgacagt
     3421 agtagtactc atgaacctca agccgtgctc cacccgctcc catggctttg ccgtttgtgc
     3481 gatagaggta gacgcaagtg ttgccgggaa gaccgttgga agttccagtg ctggagaatt
     3541 catcacttgg ggttctgtga ggagcaaccg ttccagcatt tggcccaaga ctgtccgcct
     3601 gaggtttact ggcgtagtcg gtatattcac caacgcgatt cgcgttactc gctgaaacgt
     3661 ccgcaccccc gaaacgatcc caaccgcaag cacgtaggta gccttggcgg aatcgacgca
     3721 cagcccggaa agctttttgg aagcagtctt tccaaaccac ttttccaggt cctgccactc
     3781 tttccccggc aaagaacgcg catcataggg tgcggcacgt ccttcctgca catcgctttt
     3841 ggacttgtct tttttttgcg ctcccgcact ccacttcttg ttcaattcat cccactggcg
     3901 cgccaaatcc tgcgcactgg cacgatcagc gccgccagcc gcgccgccgc cgtgggctcg
     3961 atgggctgaa gcgcgatata tacctcgcat cctccctgcc ccgccgcagc ttctgatgga
     4021 gaagatggcg ctgcaacgtt ttgtgcttga cccacaggag cacagaacaa gctggtagtc
     4081 acgatggcac agcaaaacaa accgaagccg cgcatacacg gcctccttag acgcgacagt
     4141 agcggcggag accatcgccc gaacggctcg ccttcgccct aatccgcgac cggcgcatta
     4201 tacctcgcct tgacaggacc gcgagtagtg ggtcagtttc cggtaattcg acttatcgtc
     4261 cactggcatg ctcgtttcct attgacactc tatacgtaga tcaatattat ccctatagag
     4321 agccgatagg aggcataaat tgagccctga accgacaagc cttttgcaag ggactttgga
     4381 cctgctgatc ttgaaatcgc ttatcgctgg cgagatgcac ggattgggaa tttcgcgcag
     4441 aatccagcag atcacaggcg gcacttttgt cgttaagcca ggctcgctct ttcccgctct
     4501 ccaccgaatg gaagaagaag gttggatttc ctctttttgg ggagactccg aaaacaaccg
     4561 ccgcgcgaaa tactaccgcc tgaccaagcc tggacggaac caactcgatg tcgaaacgaa
     4621 gcgatggggc cgcatttctt gggctatcgc tcaagctttg gaagcatcgt agggaggtac
     4681 ttagcatgcc gctccttgtg aaagtccgaa gtttcctgag aaacctcttt ttatctcggc
     4741 gtgtggaggt agacctcgac caggaggttc attcccacct cgaaatgctg atagcggaaa
     4801 acattcgcgc gggcatgccg ccgcacgaag cgcaacgcgc cgcgcggatc gaacttggcg
     4861 gtatagaaca ggtcaaagaa caagttcgcg aagaacgaat tggcaattgg ctgcactccg
     4921 tttactccga ttgtcgctac ggtgttcggc agctccgcaa gaacctagcc gccaccgtca
     4981 tcatggtgtt tacgctcgca ctggccattg gcgcaaccac ggcaatcttc agcgttgtat
     5041 acggagtgtt gctgaggccg ctgccgtacc ccgatgcgaa ccggatcatg gccgtcttcg
     5101 aggtcacctc gaagggcagg ccgtcgcggg ttgcggaccc gaacttcgac gactttcgcg
     5161 atcaaagccg cagtttccag gcaattgcga agtacaacga aaacgtggcg tccgtttcgg
     5221 gtgcttcgca gccgacgcgc acgacggttg cagacgtctc ccccgacttt cttagggttt
     5281 ttggtattca gccgatcctc gggcgggatt tcagcgccag cgacgcgaaa aaaggggccg
     5341 gccctaccgt ttttgtcagc tatggatact ggaggcagca tctcggatcg tcgccggatc
     5401 tctcgcagtc acacttgaag attgacggcg gcgtcttctc cgttatcggc gtgctcccgg
     5461 ccggatttcg tttcccagca gacgtggatc tgtggctgcc ggccgatttg gacggcgaaa
     5521 acccaagcag gacgtcgcat aactacaatg ccgtcgggcg cctgcgcaac ggtgtgacgg
     5581 tcgagcaagc gaatcgagac atcagcgcga tagcgcgtcg cattcacgat acctcgagcg
     5641 agcaaggcga ttatctcctc aaagatggaa ttgtcgtccc gctgcaggac tcgatcacgg
     5701 gcagggctcg ctccgcactg ctggtcctgc tcggtgcggt gggatttctg ctgctggtgg
     5761 cgtgcgcg
//
LOCUS       DASYVO010000449         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_148425, whole genome shotgun sequence.
ACCESSION   DASYVO010000449 DASYVO010000000
VERSION     DASYVO010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148425"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1143
                     /locus_tag="VGR03_15430"
     CDS             join(204..538,537..1143)
                     /locus_tag="VGR03_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611376.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV1995723.1"
                     /translation="MQAYSQDLRERVLRALDRGERPTEIARRFEVSRVWVYQVRDREQ
                     ETGVRSRFQIGGHRRSRLAEREQVLRAWIAAEPDLTLAELQQRLAQQRVAIKIGALWH
                     QLNKWNLTFKKKTLHASEREREDVQAARRAWSEAQPGMDLEKLVFIDETWTSTSMTRR
                     YGRAPRGQRCIASAPHGHWKTTTFVAGLRHQQLTAPLVADGPIDGEMFLAYVRQFLCP
                     TLRLGDIVILDNLSSHKVAGVKDAIATAGATLLYLPPYSPDLNPIEKLFAKLKALLRK
                     AAKRSIEALWNEIGELLNSLSSTECANYFASSGYVCT"
     gene            1184..2548
                     /locus_tag="VGR03_15435"
     CDS             1184..2548
                     /locus_tag="VGR03_15435"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEV1995724.1"
                     /translation="MGRTQMSKRELGRVEVLARVRSRELKLVHAVSLLRVSYRQAKRL
                     WKRYGDEGAAGLKHRSAGRSSNRAHDEKFRQKVLRRVREKYGGAVGERFGPTLAAEHL
                     ASEDGLKIHAETLRRWMLAEGLWSRERKRRRHLLRRERKEHFGEMVQMDGSFHAWLEE
                     RGPRGCLMDLVDDATSTTWAQLGEQETIWAVVDALRAWMERYGVPLAVYVDWKNLYKR
                     PATVKEQLRGEEPVTQFGRMCAKLGIEVIAASSPQAKGRVERQHGTHQDRLVKKLRRK
                     EIASHQAANVYLETEYLVEHNRRFARAAAKAEDYHRRAPRAAELDRIFRLENERTISN
                     DGVVRYQNRWFQLAPQSRHGAPSQDKVLVCEGRHGNIAIEYRGRAQRWQEIPAPAKPS
                     VVEAQPVVERASEPKPAVAKRKWVPPSNHPWREAARRGKQQRERKLAGIAARPSSTWP
                     SASP"
BASE COUNT          628 a          725 c          943 g          452 t
ORIGIN      
        1 ccccgggctg tttagccaag atctcaccaa ggtccggacg cgcacttcgt atggaagcga
       61 agagcaggcc aatggctctt caactgacat caaagctaag gaactcgggc aggaccttta
      121 gcgaattcgc caattagagc atttctaatt taagtatata cattttatgc gataatcctc
      181 ctgctggtag aggggaggca tggatgcagg cctattcgca ggatttgcgc gaacgggtgt
      241 tgcgggcgct ggatcgcggg gagcgtccga cggagattgc gcggcggttc gaagtgagcc
      301 gcgtttgggt ctatcaagtg cgcgaccggg agcaagagac gggcgtgcgc agccgttttc
      361 agatcggtgg ccatcgccgc tcgcggctgg cggagaggga acaggtgctg cgcgcctgga
      421 tcgcagccga gcccgatctg actctggccg agttacagca acggctggcg caacagcgcg
      481 tggcgatcaa gatcggcgct ctgtggcacc agttgaacaa gtggaatctc accttcaaaa
      541 aaaaccttgc acgccagcga gcgagagcgc gaggacgtgc aggcggcgcg gcgcgcctgg
      601 agtgaagcgc agcccgggat ggacctcgaa aagctggtgt ttatcgatga aacctggaca
      661 tcgaccagca tgacgcgacg ctacggacgt gcccccagag ggcagcgttg tatcgcttcg
      721 gccccgcacg gccattggaa aaccaccacc tttgtggccg gcttgcgcca ccagcaattg
      781 accgctccgc tggtcgccga cgggccgatc gatggcgaga tgtttcttgc ctatgtacgc
      841 caatttctgt gcccgactct ccggctcgga gacatcgtca ttctcgacaa tctcagcagt
      901 cacaaggtgg caggagtcaa agacgccatc gccacggcgg gtgcgacgct gctctatctg
      961 ccgccctatt cgcccgacct gaaccccatc gaaaagctct tcgccaaact caaggccctg
     1021 ctccgcaaag ccgccaaacg atctatcgag gcgctgtgga atgagatcgg cgagctgctc
     1081 aactccctct cctcaaccga atgcgcaaac tacttcgcct cttctggata tgtatgcact
     1141 taaattagaa atgctctaat aggacctccg agaggaggtc aaattggggc ggacacagat
     1201 gagcaagaga gaattgggaa gagtggaagt gctggcgcga gtgcggagcc gagagctgaa
     1261 gctggtgcat gcggtttcac tgctgcgggt gagctaccgg caggcgaagc ggttgtggaa
     1321 gcggtatggg gacgaaggag ccgcggggct gaagcaccgc agcgcggggc gaagctcgaa
     1381 ccgcgcccac gacgagaagt ttcgacagaa ggtgctacga cgggtgcggg aaaagtacgg
     1441 cggagcggtg ggcgagcgct ttggtccgac gctggcggcc gagcatttgg cgtcggagga
     1501 cggactgaag atccatgccg aaacgctgcg gcgctggatg ttggcggagg ggttgtggag
     1561 tcgagagcgg aaacggaggc ggcaccttct ccggcgcgag cgcaaagagc attttggaga
     1621 gatggtgcag atggatggca gctttcacgc ctggctggaa gagcggggcc cgcggggctg
     1681 cttgatggac ctggtcgacg acgccaccag cacgacgtgg gcgcaactgg gcgagcaaga
     1741 gacgatctgg gcggtggtcg atgcgttgcg ggcatggatg gaacggtacg gagtgccgct
     1801 ggcggtctat gtggattgga agaacctgta caaacgtccg gccacggtga aggagcagtt
     1861 gcgaggagaa gagccggtca cgcagtttgg acgcatgtgc gcgaagctgg ggatcgaggt
     1921 gatcgcggcc agttcgccgc aagccaaggg acgagtcgag cggcagcacg gcacgcatca
     1981 ggaccggttg gtgaaaaaac tcaggcggaa ggaaatcgcc agtcaccaag cagcgaacgt
     2041 gtacctggaa acggaatatc tagtggagca caatcgccgg tttgcacggg cagcggcgaa
     2101 ggcggaggac taccatcgcc gcgcgccgcg ggcggcggaa ctggaccgga ttttccgatt
     2161 agagaacgag cggacgatca gcaacgacgg ggtggtgcgg tatcagaatc ggtggtttca
     2221 actggcgcct cagagccggc acggggcgcc ctcacaggac aaagttttag tgtgcgaagg
     2281 acggcacggg aacatcgcga tcgagtatcg aggacgcgcg cagcggtggc aggaaattcc
     2341 ggctcccgcg aaaccgagcg tggtggaggc ccaacctgtg gtggaacgag ccagcgaacc
     2401 gaagccggca gtggcgaagc ggaagtgggt gccgcccagc aaccacccgt ggcgagaggc
     2461 ggcccgccga gggaagcagc agagagagag gaagctagca gggatcgcgg cgcggccgtc
     2521 gtcgacctgg ccctccgctt cgccctaaac gttccgccct tcgggctcca cagggctacg
     2581 ctccgggcca ggccgacgac aaagaaaaga agaaaaacga aaagcaaaaa ggggacattt
     2641 ctaacgaggt aaggaagggg acattttaaa agaggtttga caatcgtcga gagaaatcat
     2701 cgactgcgcg gatgtaataa cgtatggtac tttcagagta gttgcgac
//
LOCUS       DASYVO010000450         6540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_96180, whole genome shotgun sequence.
ACCESSION   DASYVO010000450 DASYVO010000000
VERSION     DASYVO010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6540
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96180"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VGR03_15440"
     CDS             complement(<1..643)
                     /locus_tag="VGR03_15440"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995725.1"
                     /translation="MTLAASDWAAIVGYLLITLFLGLYFRGRSGKSTADYFVSGRNVS
                     WWLAGTSMVATTFAADTPLVVTGLVYANGVAGNWLWWSFLPSGMMTVFLFARLWRRSG
                     LITDVQFAEMRYSGKPAAFLRGFRAVYLGLLMNCLILGWVTKAMVNIISTSMGISDFK
                     ALLICVFFLMPFTGIYVSLGGLWGVLWTDLFQFVLKMAIVISVAWYGVRAVGGM"
     gene            complement(768..2252)
                     /locus_tag="VGR03_15445"
     CDS             complement(768..2252)
                     /locus_tag="VGR03_15445"
                     /inference="COORDINATES: protein motif:HMM:NF006560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV1995726.1"
                     /translation="MSRSIVRRSWKWAAAVLFLVAAVGMSAGSGTSNGTETYDLVIAN
                     GRVMDPESGLDAVRNVGIRNGKIVAISIGPLEGNQTIEAKGLVVAPGFIDLHEHAQEP
                     KNYEFQAHDGVTTSLELEVGTADVDKWYARREGKALINFGVSIGHIPVRMRVMKDPGE
                     FLPVGDAAHREATPGEVTQIDNDIEKGLQRGALAVGMGINYTAAATHDEIFDVFQLAA
                     KQGASVHVHLRHAGLQEPETGLVGLEEVIAAAASTGAPLHVVHITSMGLKNTPRLIAI
                     VKGARARGLDVTTECYPYTAGSTRLESAIFDPGWQERQGITYKDVQWANTGERLTAEI
                     FDKYRKEGGTVVIFSIPEEAVRTAVADPIIIIASDGIPVTGPKVHPRGQGTFSRVLGH
                     YVREEKVLDLMTALKKMTLMPAQRLEKHAPTFKDKGRIRVGADADITVFDANRIIDKA
                     TYEAPLQYSEGIQFVFVNGVAVVKDGQLVDGVFPGRAARAPVSQ"
     gene            complement(2449..2646)
                     /locus_tag="VGR03_15450"
     CDS             complement(2449..2646)
                     /locus_tag="VGR03_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995727.1"
                     /translation="MKTQELKYVTRRRAAVLLGLSETELSRISSESGFGHKEVAGEQE
                     ETYFTYEELRQICMLAVHQVH"
     gene            complement(2658..2864)
                     /locus_tag="VGR03_15455"
     CDS             complement(2658..2864)
                     /locus_tag="VGR03_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995728.1"
                     /translation="MDNAFKAWVGQPVVLQVALGDIKVPLRGKLLKDGGETVRMRIGE
                     GWDVDIYKAMILAVEEDSMVLLPA"
     gene            3080..3988
                     /locus_tag="VGR03_15460"
     CDS             3080..3988
                     /locus_tag="VGR03_15460"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEV1995729.1"
                     /translation="MTRYGHASRKVLNESAVAMSNRAGVGLALGGGFARGFAHLGVLQ
                     VLEQHHIPISHIAGTSVGSILGAAYASGAPLARIIETCRTLRFRDIARWRVSRLGLAS
                     NQRLENLIERVFESRQFEELRIPLAVVATDLTSGEPVVFTQGNLVDAIRASCAFPGLF
                     EPVEIGTRCLADGGLVAPVPTRAARDLGAAAVIGVSVGMQDGYRGAPTNIFQVVSRAV
                     SAAQKHQLEIWERHADLVLRPDVQSLAWDDFERADEAIAAGAAAALRALPRIEKLLGQ
                     KEDAAQHLQARLEAEDKSYLWLAEAH"
     gene            4179..4862
                     /locus_tag="VGR03_15465"
     CDS             4179..4862
                     /locus_tag="VGR03_15465"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEV1995730.1"
                     /translation="MLDAILHFFHSLYNPEGLKELIRSGGAPLICFIVFIETGFFVGF
                     FLPGDSLLVTAGIFSAAGVIPLKWLLLPVMFCAIAGDQIGYWIGRSAGAALYRREDSF
                     FFRRSHLQRAHDFYEKYGGRAVILARFIPIVRTFCPPVAGAAKMPYLSYLVFDIFGGV
                     FWVGALILGGYFLGHLVPNIGERIHYVIAVVIVVSVLPAVISIYRARHKFGATDDRGS
                     GATSRPEGQ"
     gene            4866..5486
                     /locus_tag="VGR03_15470"
     CDS             4866..5486
                     /locus_tag="VGR03_15470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEV1995731.1"
                     /translation="MAVAKFRALIFDIGRVLIRVDVSRAMDGLASGLSLTPQEVWSAI
                     EKDPLWLDWQEGRISPRDWHLHLANRLGASLTFEQFSEVWNRALDPKPIHSESFLEKL
                     SKNYRLALLSNTDPIHMSHEEARFPFFRFFPVRIYSYRVGASKPDPLIYREALKACKV
                     RAEEAVYIDDVAAYAEAAQRLGMTGIVFQSPEQLQSDLRKLGIQFD"
     gene            5706..>6540
                     /locus_tag="VGR03_15475"
     CDS             5706..>6540
                     /locus_tag="VGR03_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995732.1"
                     /translation="MSDERIVETRTESPAWTLPAIIVLGLLAVGGLAFGWNASSKLDA
                     TQQSVATQLKSTQQAVQQDMSSLKDRLAQDEKASTDFQGDLKVVTGKLKITQGQLKKA
                     RDEAAKLNDATTQKLTALDTSVHSELATKATSDDVKVVDTKVVGVKTDLDATREDLKM
                     ARSEMGTLIARNHDEIDQLRRLGERDYVEFTITGKNKPVKVSNVTVELKGVNEKRNQF
                     SVAMTFEDKRFEKKNRAMNEPIFFYTSGTHIPEEIVINKVSKDTISGYVSVPKANSQT
                     AA"
BASE COUNT         1342 a         2056 c         1736 g         1406 t
ORIGIN      
        1 gcatcccgcc gacggcgcgg acgccatacc aggccaccga aatcacgatg gccattttca
       61 agacgaattg aaatagatcg gtccagagaa cgccccacaa accgccaagc gaaacataga
      121 tgccggtgaa aggcatcaaa aagaaaacac aaatcagcaa ggctttaaaa tcactgatgc
      181 ccatcgaagt gctgatgatg ttgaccatgg ccttagtgac ccagccgaga atcaggcaat
      241 tcatcagcaa gccgagatag acggcgcgga aaccgcggag gaaagcggcg ggcttgccgg
      301 agtagcgcat ctccgcaaat tgaacatcgg tgatcagtcc ggagcggcgc cacagacgcg
      361 cgaagaggaa gacggtcatc attccagacg gcagaaagct ccaccacagc cagttgccgg
      421 cgacgccgtt ggcgtaaacc agcccggtaa ccaccagtgg cgtgtccgca gcaaaagtgg
      481 tcgcgaccat ggaagttccc gccagccacc aggaaacgtt gcggccggac acgaaatagt
      541 ctgcggtgct cttgccggag cggccgcgga agtacaaacc gagaaataag gtgatcagca
      601 gataaccgac gatggcggcc caatccgagg cggcgagtgt caaagtgcgt cctttctcta
      661 caaagcgatt gcgcgcagta ctatatagca cttccgggtg gttggcagca tggtcttttc
      721 tggttcttgc ccagcgtcat gggggtgcgg agcgtgattc agccaagtca ctgcgaaacc
      781 ggcgctcgcg cagcccggcc ggggaaaact ccatcaacaa gttgtccatc tttgacgacc
      841 gcaactccat tgacaaacac aaactgaatc ccctccgagt attgcaaagg cgcttcgtac
      901 gtcgctttat caatgatgcg attcgcgtcg aacacggtaa tgtccgcatc cgcccccaca
      961 cgaattcgtc ctttatcctt gaacgtcggc gcgtgcttct ccagccgctg cgccggcatc
     1021 agcgtcatct tcttcagcgc cgtcatcaag tccaaaacct tttcttcccg cacgtaatgg
     1081 ccgagcacgc gcgagaacgt tccttgcccc cgcggatgaa cctttggccc ggtgacgggt
     1141 atgccgtcgc tcgcgattat aattatcggg tctgcaaccg cggtgcgtac ggcctcttca
     1201 ggaatcgaaa aaatgacaac ggtgcctcct tcctttcgat atttatcaaa aatctccgca
     1261 gttaatcgct cgcccgtgtt cgcccactgg acgtctttgt aggtaatgcc ctgccgctcc
     1321 tgccatccag gatcgaaaat tgctgactcc agtcgagtgc tcccagccgt atagggatag
     1381 cactccgtgg tgacgtcgag tccgcgcgct cgcgcgccct tcacgatcgc gatcagccgc
     1441 ggggtgttct tcagtcccat gctggtaatg tgcacaacgt ggagcggcgc ccccgtggac
     1501 gcagcggcag cgatgacctc ttcgagcccg acgagacccg tctccggctc ttgcaatcct
     1561 gcatgccgca gatggacatg caccgacgcg ccctgttttg ctgccagttg gaacacatcg
     1621 aagatttcgt cgtgcgttgc ggcagctgtg tagttgatgc ccatcccaac ggccagcgcg
     1681 ccgcgctgca agcccttctc gatgtcattg tcgatctgcg tcacttctcc cggtgtggcc
     1741 tcgcggtggg cagcgtcgcc aacaggcaga aattcgcccg ggtctttcat gactctcatt
     1801 cgcactggaa tatgcccgat cgacacgccg aagttgatga gggcttttcc ttcccgccgt
     1861 gcataccatt tgtcgacgtc cgccgtgcca acttccagct ccaatgacgt tgtcacaccg
     1921 tcgtgcgcct gaaactcata attttttggc tcttgcgcat gttcatgcag atcaatgaat
     1981 ccgggcgcaa caacgagccc cttcgcttca atcgtctgat tcccttcaag aggcccaatc
     2041 gaaatcgcca cgattttccc gttgcgaatc ccaacgttgc gcaccgcatc caaccctgat
     2101 tccgggtcca tcactcgccc attcgcgatg accaggtcat aggtttcggt tccgttgctt
     2161 gtgccgctac ctgcagacat tccgacggcg gcaaccagaa acagcacagc cgctgcccac
     2221 ttccacgatc tacgaacgat tgagcgtgac attagacctg cgtccctccc gtgcggcgct
     2281 aagtctcttc gcgcggactc tgtctgtcaa aggttttcta gaccgattca gtcaatgcga
     2341 attgctctcg cgaaattttc ggtaaaaaga aaaaccccgg atcgctgggg ggagcgaccc
     2401 ggggtggggg tattgctgag gggggggccg gaggaaactc gtttttgtct agtgcacctg
     2461 gtgtacggcc agcatgcaga tttgtcgcag ctcttcgtag gtgaaatacg tctcttcctg
     2521 ttcgcctgcg acttctttgt gtccgaatcc cgattcgctc gagatgcggc tcaattccgt
     2581 ttccgacaga cccaacagca ccgccgcgcg gcgccgggtt acgtatttga gctcttgtgt
     2641 tttcatggcg gtatccctca cgcaggaagc agaaccatgg agtcttcttc cacagccagg
     2701 atcatggctt tatagatatc cacgtcccag ccttcgccga ttcgcatccg tacggtttcg
     2761 ccgccatctt tcagcagttt cccgcgcagc ggcaccttga tatctcccaa cgccacttgc
     2821 aagaccaccg gctgcccgac ccaagctttg aacgcattgt ccattcttgg ccctctcagt
     2881 gattcagcct gacgggagag agaatcgcga aatgcgctac cgctcacaat ggtacagaga
     2941 gggcagcgga ctagtaagaa ggtactgtca atgatgccag catcgattcg cgcgcaattt
     3001 ccatcgaagt acagaagtac tgtttcttcg ggacgtgcga accataggac gaggatgttt
     3061 tccctgcgat cctgctggta tgacgaggta cgggcacgct tcgcgcaaag tcctgaacga
     3121 aagtgcagtc gccatgtcca accgggcagg cgtcggcctc gccctgggcg gaggtttcgc
     3181 gcgcggcttc gcgcatctcg gcgttttgca agtcctcgag caacaccaca tccccatctc
     3241 tcacatcgct ggaaccagcg tgggcagcat cctgggcgct gcctacgcaa gtggcgcgcc
     3301 tcttgcgcgc atcatcgaga cctgccgcac cctccgcttc cgggacatcg cccgttggcg
     3361 cgtctcccgc ctcggcttgg caagcaatca gcgtcttgag aatttaatcg aacgcgtttt
     3421 cgaatcgcgc cagtttgaag agctccgcat tcctcttgcg gtggtagcca cggacctgac
     3481 ctccggcgaa cccgtggtgt tcacgcaagg caaccttgtg gacgccatcc gcgcgagttg
     3541 cgccttcccg ggcttgttcg aaccggtcga gatcggcacg cggtgtcttg cggacggtgg
     3601 gctcgtcgcc cctgttccaa ctcgcgccgc acgcgacctc ggtgctgccg cggtaatcgg
     3661 cgtctccgtc ggcatgcagg acggctaccg cggcgccccc acgaacattt ttcaggtcgt
     3721 gagccgggcg gtgagcgcgg cgcaaaagca ccagctcgaa atctgggaac gccatgctga
     3781 cctggtcctc cggcccgacg tgcaatccct ggcctgggac gacttcgagc gcgccgacga
     3841 agctatcgct gcgggcgccg ctgccgcgct ccgcgctctc ccgcgtattg aaaagctgct
     3901 ggggcagaag gaagacgccg ctcagcatct ccaggcacgg ctcgaagctg aagacaaaag
     3961 ttatctctgg ctcgcggagg cgcactgatg actcgcgtca gcatctggct tgcagctctc
     4021 accgttccac tccttctctg gcatgaatgt ggagaggatg ttggggtttt ctgctaccgt
     4081 atagggctgg gtgcttttac gaacttgcgc tcacgcgcgc tcgttcccgt gcccgtaaca
     4141 gattcacttc atccatccgc cgcgaggaac aacactgagt gctcgacgcc atccttcact
     4201 ttttccactc gctctacaac ccagaaggac tcaaggagct aatccgctcc ggcggcgcgc
     4261 ccctcatctg cttcatcgtc ttcatcgaaa ccggtttttt tgtaggattc tttctcccgg
     4321 gtgattccct gctggtgacc gccggaattt tctccgctgc cggcgtcatt cccctgaagt
     4381 ggctgctcct gcccgtcatg ttttgcgcca tcgcgggcga tcagattggc tactggattg
     4441 gccgctcggc gggcgcagct ttgtaccgcc gcgaagactc tttcttcttc cggcgcagcc
     4501 accttcaacg cgcccatgac ttctatgaaa agtatggagg ccgcgccgtc atcctcgcgc
     4561 gcttcattcc catcgttcga accttctgcc cgcctgtcgc cggcgccgca aaaatgcctt
     4621 accttagcta cctggtcttc gacatttttg gaggagtttt ttgggttggc gccctgattc
     4681 tcggcggcta ctttctcggc cacttggttc caaacattgg tgagcgcatc cattacgtga
     4741 ttgccgttgt catcgtggtt tccgtgcttc ccgcggtcat cagcatctat cgcgccaggc
     4801 ataagtttgg cgctaccgat gaccggggct ccggcgcgac ttcccgtcca gaaggccaat
     4861 agacagtggc agtcgccaag ttccgcgccc tcatcttcga tatcggccgt gtcctgatcc
     4921 gcgtggacgt ctctcgtgcc atggacgggc tggcttccgg cctctcgttg acacctcaag
     4981 aggtgtggtc cgcgatcgaa aaggatcccc tctggctcga ttggcaggag ggacgcattt
     5041 ctccccggga ctggcacctt catttagcca atcgtcttgg cgcttcactc actttcgagc
     5101 aattcagcga agtctggaat cgcgccctcg acccgaaacc gatccattcg gaatcgttcc
     5161 tggagaagct ctccaagaac taccgcctcg cactcctttc caataccgac cccatccaca
     5221 tgtcccatga ggaagcgcgc ttccccttct tccgtttttt tcccgttcgt atctactcgt
     5281 accgcgtcgg cgcgagcaag cccgacccgc tcatctaccg cgaagccctc aaagcctgca
     5341 aggtccgggc cgaggaagcc gtttatattg acgacgttgc cgcctatgcg gaggccgctc
     5401 aacgcctcgg aatgaccggg atcgtcttcc aatcgcctga acagctccaa tccgacctgc
     5461 gaaagcttgg aatccaattc gactgatccc tcgccaagca aaaaatccgc tgttcgctct
     5521 ctcttcgcgt aaactttgct aggactagta ctgcctcctc gtttctccgt ccccacaacc
     5581 gtccatccct ctatcactta ccgagtccct cgcttctcct cctttggctt cgtgcatgca
     5641 atctctttat tacgtgccga tgaacaaggc atttccccaa attggggtga ggagagagat
     5701 tgactatgtc agatgaaaga attgtggaaa cgcgtacgga atcgccagct tggacgttgc
     5761 cagcgatcat cgttctgggc ttgctcgccg ttggtggact ggcatttggc tggaatgctt
     5821 cttcgaagct cgatgccacg cagcagtcgg ttgcgacaca gttgaagagt acacaacagg
     5881 ctgtacagca agacatgtcc tccttgaagg accgccttgc tcaagacgaa aaggccagca
     5941 ctgattttca gggcgacctg aaggttgtca ccggcaagct gaagattacc cagggccagc
     6001 tcaagaaggc tcgcgacgaa gcggcaaagc tcaacgatgc cactacccag aagctgaccg
     6061 cgctggacac ctcggtacac tcggaactcg cgaccaaggc caccagcgat gacgtcaagg
     6121 tcgtggacac caaggtcgta ggcgtcaaga ccgatctcga cgccacccgc gaagacttga
     6181 agatggctcg cagcgagatg ggcacgctca tcgctcgcaa ccatgacgaa atcgaccaac
     6241 tccgccgcct cggcgaacgc gactacgttg agttcaccat caccggcaag aacaagccgg
     6301 tcaaggtgag caacgtcacg gtcgagctca agggcgtcaa cgaaaagcgc aaccaattca
     6361 gcgtcgccat gactttcgaa gacaagcgct tcgaaaagaa gaaccgcgcc atgaacgagc
     6421 cgatcttctt ctacacctcg ggcacgcaca ttcccgaaga gatcgtcatc aacaaggtga
     6481 gcaaggacac catcagcgga tacgtcagcg tcccgaaggc gaattcgcag acggccgcta
//
LOCUS       DASYVO010000451         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_148524, whole genome shotgun sequence.
ACCESSION   DASYVO010000451 DASYVO010000000
VERSION     DASYVO010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148524"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            344..>3170
                     /locus_tag="VGR03_15480"
     CDS             344..>3170
                     /locus_tag="VGR03_15480"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1995733.1"
                     /translation="MWGRLHREICPCSRMAYNKSKNVEAAQKLLNQGKVAQAIAEYQN
                     ILKFEPRDQVTLMTIGELYIRQGETFQAIDYFERLAQIFVGDGFLTKAIAVYKRIAKL
                     APEEIRPLEKLADLYVQQGVMSEARPLFLQLAEIHLKNNRQPEAVGLLKKLLQAEPDN
                     LRIQIRLADLYQAMGQTREAIEAYVSASQRALARGDQAESEKLADKALKLEPNNLAAI
                     IVKARSYSSQGNLSKAAQLLEQAADLEKGGEQTELLLDLYLKNSDWDQASALAHRVFE
                     ADEKNFGPMQKVVESLLQSGNGEKATEILEKSRLPMIDAGEHEVVGKLLNELAASMPG
                     SVEPLEWLVELYGRTSDSFRLPDALAHLGDALVADQQYERAKKIFEQLVDREPESDSA
                     KRKLNHVLRKMGLLAPGEEEELPEENLQAEIPQPPAAKLRAETEESAPEIQAKPSVTS
                     SEPALDEETQKFIAQSLTDVDLFASYGLTQKAIGLLEAILRRAPRHTPTLEKLLDFVL
                     GAGDDRRTAELAAQLEQIHSETGDQRSSERFGELRRRFQRAAGLTDAELAGAVPGAKP
                     AAPKAAEPEPMDVSIPEIAAEPATEEPAPAPVPVQAAPVKEVRPEPVEANAAPVQAEV
                     QEVDLSDEWATLLEASRPSEATVQAPAPVPAKPAAGPTRHAEPGDVEEFQIGDEPQIV
                     SPHDVPSVAALDLAGAAPAIPLEPEPIVEKAGKKSAAAKSKEVPVAKAPQKPPETSEP
                     EFELEQEYELILEAEPLVPAYDQRPPETPIAPPQPEPVAQNTAPAPPAGSGFASDQFL
                     ADLASEIDQLGIGGLAPGFSGTMPNAQPASVAKPGAVPAQSSESGPLKEVFDEFRAEL
                     GEMGAEDEDLETHYNLGIAFREMGLLEEAISEFQKVAKASDRGRPFRYTMQCCTLLGL
                     AFMEKGQPAIAAIWYERALQTPGNDPES"
BASE COUNT          723 a          976 c          918 g          553 t
ORIGIN      
        1 agcgaggatg cagctgctcc accaagggcc gcgagaaaat tacgacggga ttcgaacgga
       61 tacatgggat gttctctccc cttccgcgac atggactatt ccgggatggt atcactttct
      121 gttgcaagag cagcccgccg atggcccaga atcagggggg tgcgcaggaa aaagagtttc
      181 ccatctcttc gccttacctt cgccatagga tttccagcat ggaaagggga taactgcctg
      241 ctcacaagcg attctgcgta cgttgacttg tctttttctc gcctgttagc gtaaccaggt
      301 agcgttacca gccgttccgc taccgcagaa gattgaggag aacgtgtggg gcagactcca
      361 tagggagatt tgtccctgtt cgcgcatggc ctataacaag agcaaaaacg tcgaagccgc
      421 acaaaagctg ctgaaccagg gcaaggtcgc ccaagccata gccgaatacc agaatattct
      481 gaagttcgag cctcgcgatc aggtcacctt gatgaccatc ggcgagctgt atatccgcca
      541 gggcgagacg ttccaggcca tcgactactt cgaacgcctg gcgcagattt ttgtcggcga
      601 cggattcctg acaaaagcaa tcgcggttta caaacgcatc gccaagctgg ctcctgaaga
      661 aatccgccca ctcgaaaagc ttgccgacct ctacgtgcaa cagggcgtga tgagcgaggc
      721 ccggcccctt tttctccaac tggcagagat tcacctgaaa aacaatcgcc agccggaagc
      781 cgtgggcctt ttgaaaaaac ttctccaggc ggaacccgac aatctccgca tccagatccg
      841 cctagccgat ttgtaccagg ccatgggcca gactcgggag gccatcgagg cttacgtctc
      901 cgcttcgcag cgggcgctgg cgcgcggtga ccaggctgaa agtgaaaagc tcgcggacaa
      961 ggcgctgaaa cttgagccca acaatctggc cgcaatcatc gtcaaagcgc ggtcgtattc
     1021 ttcacagggc aatctcagca aagctgcgca gcttctggaa caggcggccg atctagagaa
     1081 gggcggcgag cagaccgagc tcctcctcga tctttatctc aagaattccg actgggacca
     1141 ggcctcggcg ctcgcgcatc gcgtatttga agcggacgag aaaaattttg ggccgatgca
     1201 aaaggtcgtc gaatcgctgc tgcaatccgg gaatggcgaa aaggccacgg aaatcctgga
     1261 gaagtcgcgg ctgcccatga tcgatgccgg agagcacgag gttgtcggga aattactcaa
     1321 cgagcttgcg gccagcatgc ctggcagtgt cgagccgctg gaatggctcg tggaactgta
     1381 cggccgcacc agcgattcct tccgcttgcc cgacgcgctg gcgcacctgg gtgacgccct
     1441 ggttgccgat cagcagtacg aacgcgcaaa gaaaatattc gagcaactcg tcgatcgcga
     1501 acctgaaagc gattcggcca agcgcaaact caatcacgtg ctccgcaaga tgggactcct
     1561 cgcgccggga gaagaggagg agctccccga ggagaacttg caggcagaaa ttccgcaacc
     1621 tcccgccgcc aagttgcgcg cggagacgga agaatccgct cccgaaatac aagccaagcc
     1681 ttccgttacg tccagcgaac ctgcgctcga tgaagaaacg caaaaattca tcgcgcagtc
     1741 gctcaccgac gtggatcttt tcgccagcta cggcctcacg cagaaagcca ttgggttact
     1801 cgaggccatt ctgcgccgtg ccccgcgtca cacgccgaca ctcgaaaaac tgctcgattt
     1861 tgtccttggt gcgggcgacg accggcgcac cgccgaactc gccgcgcagt tggaacagat
     1921 ccacagcgaa acgggcgacc agcgcagctc cgaacgattt ggggaattgc gccgccgctt
     1981 ccagcgtgcc gctggtttga cggacgcgga actggcaggt gctgttcctg gggcaaaacc
     2041 tgcggccccg aaggccgccg aaccagagcc gatggatgtt tcgataccag aaatcgccgc
     2101 ggaacccgcg acggaagaac ccgctcccgc tccggtgccc gtccaggccg ctcccgtgaa
     2161 ggaagttcgt cccgagccag tcgaagcaaa cgccgctccc gtgcaggcgg aagttcagga
     2221 agtcgatttg tccgacgaat gggcgaccct tctcgaagcg tcgcgtccca gcgaagcaac
     2281 tgttcaagct cctgccccag tgccagccaa gcccgcggcc ggacccacgc gtcacgctga
     2341 acctggggac gtggaagagt ttcagattgg cgatgaaccc caaatcgttt cgccgcacga
     2401 tgtaccgtcg gtcgccgccc tcgatctcgc cggcgcagct cccgcaattc cgctggagcc
     2461 ggaaccgatc gtcgaaaaag ccggaaagaa gtccgcagca gcgaagtcta aagaagtacc
     2521 agtggctaaa gctccgcaga aacctcccga gacaagcgag cccgaattcg aattggaaca
     2581 ggagtatgaa ctcatactgg aagcggagcc cctcgtcccg gcgtacgacc agaggccgcc
     2641 cgagacgccc attgctccac cccagccgga gcccgtcgct caaaacaccg cgccagcgcc
     2701 acccgcgggc agcggttttg cttccgatca atttcttgcc gatcttgcca gcgaaatcga
     2761 tcagcttggc atcggcgggc ttgcaccggg gttctcagga acgatgccga atgcccagcc
     2821 ggcgtccgtg gcgaagccgg gggcggtgcc cgcgcagtct agcgagtccg gcccgctcaa
     2881 ggaagtgttt gacgagtttc gcgccgaact gggtgagatg ggtgcggagg acgaagacct
     2941 ggaaacgcac tacaacctgg gcatcgcctt tcgtgaaatg gggctcctcg aggaagccat
     3001 cagcgagttc cagaaagttg ccaaagccag cgatcgcggc cgccctttcc gttacaccat
     3061 gcagtgctgc acgcttctcg gtctcgcctt catggaaaag ggccagccgg ccatcgccgc
     3121 gatctggtat gagcgcgcgt tgcagacccc cggcaatgat ccggaaagca
//
LOCUS       DASYVO010000452         1931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_96279, whole genome shotgun sequence.
ACCESSION   DASYVO010000452 DASYVO010000000
VERSION     DASYVO010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96279"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VGR03_15485"
     CDS             complement(<1..674)
                     /locus_tag="VGR03_15485"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate-2-kinase family protein"
                     /protein_id="HEV1995734.1"
                     /translation="MPQLKQLARQIFHQTLAAIDIPATMQRKLQRKGTLLVCGEKRID
                     LRDFEKLRVVAIGKAAHAMVEGLALVLAPFLRLEGVVSVPTAAQKPLAGMKYFVAGHP
                     VPNAESWKAAEAILALLKKCDVKTLVFFLLSGGGSALVELPLDPAQTLEDVQNMHRAL
                     VTCGASIDDINTVRKHFSAVKGGRLAAAAHNATKFTLAVSDVPAGKESALASGPTLPD
                     PTAVADA"
     gene            complement(697..1677)
                     /locus_tag="VGR03_15490"
     CDS             complement(697..1677)
                     /locus_tag="VGR03_15490"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEV1995735.1"
                     /translation="MAKPKVFATHSLFDAARQILQETCEVEFWTKTERPPREEVLRRV
                     KDKQGLICLLTEKVNEELLRVAPKLRIAANVAVGFDNIDLAACTKRGVLATNTPGVLD
                     ETTADFAWTLLMAVARRLSEGEALARSGNWKNWDLDQLVGTDVWGKTLGIVGFGRIGR
                     AVARRASGFQMKVIYTDAQRAPAEVESALKTEFREMNALLAESDFISVHVPLLPETRG
                     LFDAPKFYQMKPTAFLINTSRGPVVDEAALVAALESGKIAGAGLDVYENEPFIHPGLK
                     RANVVLAPHIASASLETRTKMACIAAENVVALFTGQRPPHILNPEVLKAS"
BASE COUNT          359 a          687 c          463 g          422 t
ORIGIN      
        1 gcgtccgcaa ccgcagtcgg gtcaggcaga gtcggccctg acgctagtgc cgattctttc
       61 cccgccggaa catcgctcac tgccagtgtg aacttcgttg cgttgtgcgc tgccgccgca
      121 agccggccgc ctttcacggc ggagaagtgc ttccggacag tattgatgtc atcgatcgat
      181 gcgccacagg ttacgagtgc tcgatgcata ttttgtacgt cttcgagcgt ttgcgccggg
      241 tccagcggta actccacaag agccgatccg cctcccgaaa gaagaaagaa aaccagcgtc
      301 ttcacatcgc actttttcag cagcgctaga atcgcctccg ccgccttcca gctctcggca
      361 ttcggcaccg gatgccccgc cacgaagtac ttcataccgg ccaatggttt ctgcgccgcc
      421 gtgggcacgg ataccacccc ctcgagtctc agaaaagggg ccagcaccag cgccagcccc
      481 tctaccatcg catgcgccgc tttcccgatc gccaccacgc gcagcttctc gaaatcccgc
      541 agatcgattc tcttttcacc gcacaccagc agcgttccct tgcgctgcag cttccgctgc
      601 atcgtcgcgg gaatatcgat cgccgccagc gtttggtgga aaatctgccg ggcgagctgc
      661 ttcagttgcg gcatgagagg tgtgcctgga aattcgttac gatgccttca gaacttctgg
      721 attcaagatg tgcggcggcc gctgtcccgt gaacagcgcg acgacatttt ccgccgcgat
      781 gcacgccatt tttgtccgcg tctccagcga cgccgaagcg atgtgcggtg ccagcaccac
      841 attcgcacgt ttcagcccgg gatgaatgaa cggctcgttc tcgtaaacat ccagccccgc
      901 gccggcaatt ttcccgctct caagcgccgc caccagcgcc gcttcatcca ccaccggccc
      961 ccgcgatgta ttgatcaaga acgccgtcgg cttcatctgg tagaatttcg gcgcatcgaa
     1021 caagccccgc gtctccggca gcagcggcac atgcacgctg ataaaatccg attccgccag
     1081 caatgcattc atctcccgaa actccgtctt caacgcactc tccacttccg cgggcgcgcg
     1141 ttgcgcatcc gtgtaaatca ccttcatttg aaatccgctc gcgcgccgcg ccaccgctcg
     1201 tccaatccgt ccaaacccca cgatgcccag cgtcttcccc cacacgtccg tccccaccag
     1261 ttgatccagg tcccagtttt tccaattccc cgaacgtgcc agcgcttcgc cttcgctcag
     1321 ccgccgcgcc accgccatca gcagcgtcca cgcaaaatcc gccgtcgttt catccagcac
     1381 ccccggcgtg tttgtcgcca gaacgccccg cttcgtgcag gccgccaagt cgatgttgtc
     1441 gaatcccacc gccacgttcg ccgcaatgcg gagcttcggc gccaccctca gcagttcctc
     1501 gtttactttt tcggttaaca gacaaatcaa tccttgtttg tccttcacgc gccgcaacac
     1561 ttcttcccgc ggcggacgtt ccgttttcgt ccagaattcc acctcgcatg tttcttgaag
     1621 aatttgcctg gcggcatcga acaagctgtg ggtcgcaaac actttcggtt tcgccatcgt
     1681 ctcctcttcg caattttggg aaaatcacac taacacaccg ccgcgctccc ggcataactt
     1741 agcgttgcgc gcgtcccgga aatctcacta tagtgccagg tgcgcccgat cctgcgccct
     1801 atgaactccg gcctcgttcc cgaattcgag aaacttctcg gccctacttc cgttctttac
     1861 cggcccgagg atcttcttct ctatgagtac gacggctcta tcgaaaaagg ccgccccgac
     1921 gtcgtcgttt t
//
LOCUS       DASYVO010000453         1751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_96287, whole genome shotgun sequence.
ACCESSION   DASYVO010000453 DASYVO010000000
VERSION     DASYVO010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96287"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VGR03_15495"
     CDS             complement(<1..878)
                     /locus_tag="VGR03_15495"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEV1995736.1"
                     /translation="MASALALARPVNAIRGVIAGTAPISTPGAMLEDGSGTLPAAFSA
                     LKPLGARVHPITGEEFHGRLLRAQKLMAELAPKYDALFVAPGTSLYYFTGIRWGISER
                     LLALVLPRTGDPIVIVPGFEEGRMREKLQFAAEVRVWQEDESPTKIAAAALADRGIRT
                     GRVGVEETAAFTFFDHLRGAAPGFECVSADPVTIACRGRKSAHELELMRLACEATFDV
                     FRAVFASLKEGMSQEDIGKLVEGGFSKMGLHGGALVLLGASAALPHGTIKPQKLKDGD
                     VVLIDGGCAVDGYQSDV"
     gene            complement(1119..>1751)
                     /locus_tag="VGR03_15500"
     CDS             complement(1119..>1751)
                     /locus_tag="VGR03_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995737.1"
                     /translation="EVSGTYLAQSGQPVTPLSGLDSNANGDTAGDRTIFNPAGVGLTG
                     STVTPVCNNGLAGGGATYVDPKCDPNHADDKNVGGDPTPGAGAPAIVGYLADTPGARF
                     IQAGVGTKTNVGRNTVSSPGLNVWNMSVFKTNKLTERASLQFRFQTYDTFNHRNYSIG
                     LPTNSGAVDSANNTNPLNGGYIFVTSPTFLNKFSFDGGSRTLELGIKLVW"
BASE COUNT          336 a          559 c          495 g          361 t
ORIGIN      
        1 acgtccgatt ggtatccatc cacggcgcag ccgccgtcga tcagcaccac atcgccatct
       61 ttcagttttt gcggcttgat ggtgccgtgc ggcagcgccg cggatgcgcc cagcagaacg
      121 agcgccccgc cgtgcaaacc cattttcgaa aaaccccctt ccaccagctt gccgatgtcc
      181 tcctgggaca tcccttcctt caatgaagcg aacaccgcgc ggaacacgtc gaatgttgcc
      241 tcgcaggcca gccgcatcag ctcgagttcg tgcgccgact tgcgtccgcg gcacgcgatg
      301 gtcaccggat cggcggaaac acattcgaat cccggcgccg cgccgcgcag atgatcgaaa
      361 aaagtgaatg ccgcggtttc ttccacgccc acgcgtcccg ttcgaatccc tcggtccgcc
      421 aatgccgccg ccgcgatttt cgtcgggctc tcatcctcct gccacacgcg cacttccgcc
      481 gcgaattgca acttctcgcg catgcgccct tcttcaaatc ccggcacgat gacgatcgga
      541 tcgcccgtcc gcggaagcac cagagcgagc agccgctcgc tgattcccca gcggatgccc
      601 gtgaagtagt agagcgacgt cccgggagcg acgaaaagcg cgtcgtactt gggcgccagc
      661 tccgccatca gtttttgcgc gcgcaggagc cgcccatgga actcttcgcc cgtgatcgga
      721 tgaacccgcg ctccaagtgg tttgagcgcc gagaacgccg ctggcagcgt ccccgagcca
      781 tcctccagca tcgccccggg tgtgctgatg ggcgcagtcc cggctataac gccgcgtata
      841 gcattgaccg gccgcgccag cgccaaggcc gaagccatag ccgtgccctt caagaaatac
      901 ctgcgcgatg ccttcgaacg cgaatccaaa gttggtttgt gcatgtcagg caagaggttt
      961 acccggggtc accgcagatt gtcaagggag atgaaagatt tgtagtgatt gaatttaacc
     1021 gctgtcttcg gcggacaaga gctttggctg cacccaaaag caaaacgggg aggctcgcaa
     1081 gagcctcccc gtttctgttg tttccgttct ggtctggatt accagaccag ttttatgccg
     1141 agttccaaag tgcggctgcc tccgtcaaag ctgaatttgt tcaggaatgt cggcgacgta
     1201 acaaagatgt agccgccatt caaggggttg gtgttattcg ctgaatcgac cgcgccacta
     1261 ttggtcggca ggccgatgct gtaattgcga tggttgaacg tgtcgtaggt ctggaaacgg
     1321 aattgcaatg aggcccgttc cgtaagtttg ttcgtcttga acacggacat gttccatacg
     1381 tttagtcctg gcgagctgac cgtgttccgg ccaacgttgg ttttcgtgcc aaccccagcc
     1441 tggatgaatc gcgcacccgg cgtatcggcc aagtaaccga cgattgctgg cgccccggct
     1501 cccggagtgg gatcaccgcc cacgttcttg tcgtcagcgt gattcggatc gcacttgggg
     1561 tctacgtacg tagccccgcc acccgcgagc ccgttattac acaccggcgt gactgtagac
     1621 cctgtgaggc cgacgcctgc cgggttgaag atcgtgcggt cgccagcagt atctccattc
     1681 gcattcgagt ccaggcccga tagcggcgta actggttgcc ccgactgcgc caagtaggta
     1741 ccgcttacct c
//
LOCUS       DASYVO010000454         4394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_67019, whole genome shotgun sequence.
ACCESSION   DASYVO010000454 DASYVO010000000
VERSION     DASYVO010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67019"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="VGR03_15505"
     CDS             complement(<1..1347)
                     /locus_tag="VGR03_15505"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV1995738.1"
                     /translation="MSNVGQKLFIEPERYEFSAAPMHQFELVRRDFFKILGAGIAVFA
                     IAKDVLAAQETAPGHRSFHNEELPKDISAWLHIGEDGSVTGFTGKAEIGQNIRTALAQ
                     TIADELRVPFESVQMVTADTALTPFDAGTFGSRTTPTITPQLRRVAAAARDLLVRAAA
                     KEWNVAPAGLVAADAKVTDPASGRSLKYAELARGKSLAQNIPAEGSITLATQWTIAGK
                     AIPKVDGRAFVTGRHQYTTDLRPAGMLYGRVLRPSSFGATLVSCDDSAAKAMPGVFVV
                     RDGEFVGVAAPSAHEAQRALEAIHAQWNEVPQISNKELFAYLKQNAAAKSEDRFRKQK
                     GSVEEGLAGAAHRLDATYTVAYIAHAPLEPRAAMAQWADGKLTVWTGTQRPFANRDEL
                     ADVFHLPESSVRVIVPDTGSAYGGKHTSDAALEAARLARAVGRPVKVAWTREEEFTW"
     gene            complement(1344..1823)
                     /locus_tag="VGR03_15510"
     CDS             complement(1344..1823)
                     /locus_tag="VGR03_15510"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV1995739.1"
                     /translation="MAKPIELEINGKRYPVNYPPDTPLLYVLRDELGLSGSKYGCGEG
                     QCGACTVLIEGAPRRSCQIPVSAAAAKPITTIEGLEKDGRLNPVQQAFLDAGAFQCAY
                     CTSGMIMSSVGLLQTNPNPTQAEIVQFLQGNVCRCGTHPRIIEAVRQAAKMMQEHGR"
     gene            complement(1857..2423)
                     /locus_tag="VGR03_15515"
     CDS             complement(1857..2423)
                     /locus_tag="VGR03_15515"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEV1995740.1"
                     /translation="MEKRIRGIVPAVIATTLAAFLLLAAGFAAQQAVPPQAAKQMVPD
                     NPSEHTPPVQPIPYSHKKHLSLGLDCKDCHTNPEPGKLMTFPDTSKCMLCHVRVAKDK
                     PSIQKLAGYSKSKQAIPWVRVYAVLPGVAWNHRPHLEAGVKCETCHGQVREMEAMSEV
                     TSVTTMYSCLHCHEMNHAKIACDTCHKH"
     gene            complement(2469..4139)
                     /locus_tag="VGR03_15520"
     CDS             complement(2469..4139)
                     /locus_tag="VGR03_15520"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1995741.1"
                     /translation="MSTISRREFVKTTALAPLAVTRFSISPQASKEDRFDIVVAGAGH
                     NSLVTAAYLAKAGYRCLVLEGRPMLGGGVKTAELTLKGFKDDVCSTAHTFLLDNPMMK
                     NDELKLWDYGLEYIDPDPIFHIPFPDGTYLTQWRDLDRTCAEFAKFSKKDAAAYRKML
                     AEFDAVKPVLLGTSFAPIGFGKPLNDQLAQQPRGKLWQRRLAMSAWEIIRDTFEEEHC
                     RTFMLYMSHLAAEPPDAPMTGRLAYGTARQQHSGRPIPKGGSGALTVALARSIEAHGG
                     VLLTNKWVKQLIVENGKCVGVECSDGSSYRAEKAVVSTIHIKHLVDMAPRELWGEDFI
                     DGVNTWQAEHAMFVTHYATSEPPKYAVEGGTLSPVESGILVSPERALRYAYDDARGVV
                     NLEDPSMQIICCSIADPTRAPAGMHTVKVVGWQPYELKEGPEHWDKIKNEVSDAYLKY
                     LRRFAPNLTDDKILARFVESPLDLERMNPHFWHGSAHAGAQSASQSGPMRPMPGWAQH
                     RMPIPGLYQTGATTFPGGSVTGAPGRNAATVMLKDFGTSIEEVVNKKA"
     gene            complement(4165..>4394)
                     /locus_tag="VGR03_15525"
     CDS             complement(4165..>4394)
                     /locus_tag="VGR03_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995742.1"
                     /translation="NWHGSCHAGGSGPAQSGAMRPVPGWAQHRMPIPGLYQTGATTHP
                     GGSVTGGPGRNAATVMLKDFGTSIEEVVKKT"
BASE COUNT          834 a         1399 c         1202 g          959 t
ORIGIN      
        1 ccaggtgaat tcttcctcgc gcgtccacgc cactttcaca ggacgcccga ccgcgcgcgc
       61 cagacgagcg gcttcgagcg ccgcgtcgct ggtgtgtttt cctccatacg ccgagcccgt
      121 atccggcaca atcacccgca cgctgctctc gggtaaatgg aaaacatccg caagctcgtc
      181 gcggttggcg aacggccgct gcgtgccggt ccacaccgtg agtttcccat cagcccactg
      241 agccatggcc gcgcgcggct ccagcggggc atgcgcgatg tacgcaacag tgtaagtcgc
      301 gtcgagccga tgcgccgcgc ctgccagccc ctcttcgacg gatcccttct gcttccgaaa
      361 tcgatcctcg gatttcgcag cagcattttg cttgaggtat gcaaagagtt ccttgttcga
      421 aatctgcggc acttcattcc attgagcgtg tatcgcttcg agagctcttt gcgcctcgtg
      481 cgcgctcggc gcggctactc cgacaaattc tccatctcgc acgacaaaca cgccgggcat
      541 ggccttcgca gcgctgtcat cgcaggaaac gagcgtcgca ccaaacgacg acgggcgcag
      601 aactctgccg taaagcatgc ccgcaggccg cagatcggtg gtgtactggt gccgcccggt
      661 aacgaacgcc cggccgtcca ctttcggaat cgcttttccc gcaatcgtcc attgcgttgc
      721 cagagtgatg gaaccttccg ccgggatatt ctgcgcgagc gacttgccac gcgcgagctc
      781 cgcatactta agcgagcggc ccgatgcggg atcggtgact ttcgcgtcgg ctgctacaag
      841 tcccgccggc gcgacattcc attctttggc cgctgctctg acaaggagat cgcgcgcggc
      901 cgcagccacg cgccgtaact gcggggtaat cgtcggcgtc gtccggctgc caaatgtccc
      961 cgcgtcaaac ggagtcagcg cggtgtctgc cgtcaccatc tgcacactct cgaagggaac
     1021 acgcagctca tcggcgatgg tctgcgccaa agccgtgcgg atgttctgcc cgatctcggc
     1081 cttgccggta aatccagtaa cgctgccgtc ttcaccgatg tgcaaccacg cgctgatgtc
     1141 cttcggcagt tcttcattgt gaaaactccg atgcccaggg gccgtttcct gcgcggccag
     1201 cacatctttc gcaatcgcga aaactgcaat tccagcgcca agaatcttga aaaagtcgcg
     1261 gcgcaccaat tcgaattgat gcatcggcgc agcgctaaat tcgtaccgct ccggctcgat
     1321 gaacaatttc tgcccgacgt tactcatcgc ccatgctcct gcatcatctt ggccgcctgt
     1381 cgaaccgcct cgatgatgcg cggatgcgtg ccgcaacgac acacattacc ttgcaagaat
     1441 tgaacgattt ccgcctgagt gggattcgga ttcgtttgca acagaccgac gctgctcata
     1501 atcattccgg aagtgcaata agcgcactgg aaagcgcccg catcgagaaa cgcctgctgc
     1561 actggattca accggccgtc cttttcaaga ccttcaatcg ttgtgatggg ctttgccgca
     1621 gccgcgctga cgggaatctg gcacgagcgt cgtggcgctc cttcaatcaa aacggtacaa
     1681 gcaccgcact gcccttcccc gcagccgtac ttgctgccgc tcaggccaag ctcatcgcgc
     1741 aggacataca atagaggggt gtccggaggg tagttgaccg gatatcgctt gccgttgatt
     1801 tccagttcga tcggcttcgc catgatccct cctcgaaagc aaaattctcc gcccgatcag
     1861 tgtttgtgac aagtgtcgca agcgatcttc gcgtgattca tttcgtggca gtgcagacag
     1921 ctgtacatcg tggtgacgct cgtaacttcc gacatcgctt ccatctcccg aacctgcccg
     1981 tggcacgtct cgcacttcac gcctgcctcc aggtgtgggc ggtgattcca cgcgacgccg
     2041 ggcagcacgg catagacgcg aacccaaggg atcgcttgct tggacttgga atacccggcg
     2101 agcttctgaa tcgaaggctt gtccttggca actcgcacat ggcacagcat acatttgctg
     2161 gtgtcaggaa aggtcatcag cttgcccggc tccggattcg tatgacagtc cttgcaatcc
     2221 agaccgagag acagatgttt cttgtggcta taagggatag gttgcacagg cggcgtgtgc
     2281 tccgacggat tgtctggaac catttgcttc gccgcttgcg gtggcacagc ctgttgcgcc
     2341 gcaaaacctg ccgcaagtaa aagaaatgct gccagcgttg tggcgatcac cgcgggaacg
     2401 attcctctaa ttcttttctc cattggctga cctcgcaaat tagaatctgc cgcgtttctt
     2461 cgccccaact acgctttctt gttcacaact tcttcgatgc tggttccgaa atccttgagc
     2521 atgaccgtcg cggcgtttcg gccgggcgct cccgtaaccg aaccgccagg gaacgtcgtc
     2581 gcgcccgttt gatacaagcc cggaattggc atgcggtgtt gagcccagcc gggcatcgga
     2641 cgcatcgggc cggattgcga tgcgctttgc gcgcccgcgt gcgcacttcc gtgccagaag
     2701 tgcggattca tccgctcgag atcgagcggg ctctctacaa atcgcgccag aatcttgtca
     2761 tcagtcaaat tgggcgcaaa ccgccgcaga tatttcagat aagcgtcgga aacctcgttc
     2821 ttgatcttgt cccaatgctc gggaccttct tttaactcat acggttgcca gcccaccacc
     2881 ttcactgtgt gcattcccgc cggggctcgc gttggatcgg cgatgctgca gcagatgatt
     2941 tgcatcgacg gatcttcgag attcactacc ccgcgcgcat catcgtaggc atatcgcagt
     3001 gcgcgttccg gtgaaaccag aattcccgat tccaccggcg aaagtgttcc gccttccacc
     3061 gcgtacttcg gtggctcgga tgtggcgtag tgcgtgacga acatcgcgtg ctccgcctgc
     3121 caagtattca cgccatcgat gaaatcctcg ccccaaagct cgcgcggcgc catgtccacc
     3181 aggtgcttga tgtggattgt tgaaaccacc gccttttcag cacggtaaga acttccgtcg
     3241 ctgcattcca cgcccacaca ttttccgttc tccacaatca gctgcttcac ccacttgttt
     3301 gtgaggagca cgccaccgtg tgcttcgatc gagcgtgcca gcgcaacagt caaagctccc
     3361 gatccgcctt tcgggatggg ccggccagag tgctgttggc gagccgtgcc gtatgcgagc
     3421 cgtcccgtca tcggggcatc cggcggttcg gccgccaggt gggacatgta gagcatgaac
     3481 gtgcggcagt gttcctcttc gaacgtgtca cgaataattt cccaggccga catcgccagc
     3541 cggcgctgcc atagcttgcc gcgcggttgt tgcgccagtt ggtcgttcaa tggtttgcca
     3601 aaaccaatcg gcgcgaagct ggttcccaat agaaccggtt tgacggcgtc aaactccgcc
     3661 agcatctttc ggtacgcggc ggcatccttt ttcgagaatt tcgcgaactc cgcgcaagtc
     3721 cggtccaggt cgcgccactg cgtcagatag gtgccatccg gaaagggaat atgaaagata
     3781 gggtcgggat cgatgtactc aagcccgtag tcccagagct tgagctcgtc gttcttcatc
     3841 atcgggttgt cgagcagaaa cgtgtgcgcc gtcgagcaca catcgtcctt gaatcccttc
     3901 agagtcagct ctgcggtttt gacgcctccg ccaagcatcg gacggccttc caggacgaga
     3961 caccgatacc ccgccttcgc caggtacgcc gccgtaacca ggctgttgtg gcccgcgccg
     4021 gcaacgacaa tgtcgaatcg atcttccttg gaagcttgcg gacttattga gaaccgtgtg
     4081 acggccagag gtgccaatgc cgtcgtcttt acaaactcgc gtcgactgat tgtgctcatt
     4141 gaagcgcccc ctcagaccaa actcttatgt cttcttcacg acttcctcga tgctggtccc
     4201 aaaatccttc agcatcaccg ttgccgcatt tcgccccggg ccaccggtta cggatccgcc
     4261 gggatgcgtc gtcgctcccg tttgatacaa gcccgggatc ggcatgcggt gttgcgccca
     4321 gcccggtacg ggacgcatgg cgccggactg cgccggtccc gacccgcccg cgtgacaact
     4381 gccgtgccag ttgt
//
LOCUS       DASYVO010000455         5562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_7503, whole genome shotgun sequence.
ACCESSION   DASYVO010000455 DASYVO010000000
VERSION     DASYVO010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7503"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1139)
                     /locus_tag="VGR03_15530"
     CDS             complement(111..1139)
                     /locus_tag="VGR03_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995743.1"
                     /translation="MSTDTLGGFKASLSDWLGETFDSRMTNKAGEAVSDAVESLWVAV
                     ITTQISKNLGGPVSITLNAAAERLTLVSIADPTVGAVGASVAGGALAGRTYDLAYSLA
                     TESGSETNVTPRVQVVVALNNLCQVSAPTFVPGAFGWNLYFAPNTVPATPLARVNAEP
                     IDFNQLLYIEPVTGWQPVPDNPFAPAQNSTADNIAYIRHLELLTPNGTYKSWNQADLD
                     STMFRRMAGSTASTSPYQNQAWDLVNGNTLEVRPAMGATQVPRYFYVIKPRRLAFDGS
                     QIPFQNTPCASEFIRARALSRLCLSVYEYSASEMWAAIAKESKADVMKGLAQENLAKG
                     KKVTPYMY"
     gene            complement(1197..1763)
                     /locus_tag="VGR03_15535"
     CDS             complement(1197..1763)
                     /locus_tag="VGR03_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995744.1"
                     /translation="MSGSVVLLHEDHKFGTVEEQVGNVIYRIPPGEATEVPDEVMEFS
                     DAIEYRVPVAQIILRHKGPIHGIVAVTAVKMKNQLGVEYLVDEAKEEARKLLAASCQQ
                     RFEQYLQEQKLRIQQGGQAVAPSGRNALYIEEMGISLKDHGINPVGFDQSKAPSASAL
                     ASENAELKNQMSEMREQIAAIARAQKKK"
     gene            complement(1726..2403)
                     /locus_tag="VGR03_15540"
     CDS             complement(1726..2403)
                     /locus_tag="VGR03_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995745.1"
                     /translation="MEFLTAEAQGMVDVLRMTPPLEAAGIELQRQVDERLEADRAGVE
                     RVPKEVNSKLQAYDSKLRARYEFRIEGMVIERLAPEAGGWLQVSAWPEDEDRMPIPIE
                     LVIEMLGAGDMRKYRNTEEYLEKKREKAKKVKEANIKAGDSKLEEAIDSLSSKQVKQF
                     AEVERAMLTGETVIAHGDDERFLDYAHQITRRHRAEGKTDVTPAAGHFTRRGRNVRKR
                     SAVARRP"
     gene            complement(2502..3887)
                     /locus_tag="VGR03_15545"
     CDS             complement(2502..3887)
                     /locus_tag="VGR03_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995746.1"
                     /translation="MRVYTKVVMDMVSNEIIEQEFYEYDGPVAQCAVDSNTFLADMKY
                     VYGAMTDQISTLPALMNLFGDGSKVGKPINLIGIRGYVFGARMQPNWNIGFRKEGTTG
                     VGSAGNQGLANSTVLLKYDYDPQLITGQAENLSKGEARAFMQAKALTSKYDLLDMVSH
                     ANVLVAGADRGGQLGTVNASAAGSITVPALSPTSAAALYLRLGQPIDCGPVGGGALSL
                     QGAVISAINYATGVITHNGGTATVGHAIYLSGEAPLVAGDFPLTCEGLISLISDTGAI
                     QGLNPATAGQQAWASFAFDNANVQITSQGIQQLRQFTKNRGGVDVDTFIFPSAQVNQL
                     VAIATSTIRFDLNAASARGSATKRAIDLGFNVFEYAGLEIIEDKDARPDRIFAFAVET
                     LKKFEALPLSLAEDEAGTWTRVSGVNGIADAVQALLRWYFNVGIMRRSLAGVMRNCLV
                     PTTFLTAPPTF"
     gene            complement(4032..4220)
                     /locus_tag="VGR03_15550"
     CDS             complement(4032..4220)
                     /locus_tag="VGR03_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995747.1"
                     /translation="MRELDIVEVNGRIGTIVDLAALVPGRPQRALVEFPYFQFEDFAI
                     EQLKVIFTARAEQFSPAN"
     gene            complement(4282..5160)
                     /locus_tag="VGR03_15555"
     CDS             complement(4282..5160)
                     /locus_tag="VGR03_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995748.1"
                     /translation="MADELNNQAVDPGTGAEPVPAPGAPAAGADPGGGAGAGTGTPAA
                     GTPAPVETANTRQFREAFEALKAKYEPWEKLNAKPEEVATGHSAYQKISTEAHELGAT
                     LGFTLEQIDQALKDDLVGTLAWLRSEALRKEKEAGGPDVRQLVKREMEEWTKPLNEHM
                     RKQREAEATRKADEGNFTFNQEFKKQIEAKYPDEATRPGDKGGEWVYQRTCELIAKDQ
                     QALDGLNRGKVSSVAKYFEQANADYLALVGEHGTRETARVQHKPGEKKPPANGNKRPS
                     LQELIEDPGLIHKKYA"
     gene            complement(5206..>5562)
                     /locus_tag="VGR03_15560"
     CDS             complement(5206..>5562)
                     /locus_tag="VGR03_15560"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1995749.1"
                     /translation="TTDKLAPWKKGKFTGKNVKDVKGELDEYQNDKPDEPIPRGESFN
                     SYLARLKDGVSILREKQKANPGGHVVAVTHSTDLLALPHVHSAGEEDIPTSGAPKPGS
                     VSVVKTGGGKTRLEAL"
BASE COUNT          983 a         1842 c         1445 g         1292 t
ORIGIN      
        1 ttgaacttct tcaccgtttc cggtaccagg aaacggatga gcgattgcgt aagtggatct
       61 ctcggcattc ttctctcttt ctgctgactg ctgaccgctg actgctgttc tcaatacata
      121 tacggcgtca ctttcttccc cttcgccaaa ttctcctgcg ccaggccttt catcacatcc
      181 gccttcgact ctttcgcgat cgctgcccac atctctgacg ctgagtattc ataaactgat
      241 aaacacagtc tcgaaagcgc ccgcgcgcgg ataaactccg acgcgcaggg agtgttttgg
      301 aaaggaatct ggctgccgtc aaacgccagg cgccgcggct tgatcacgta gaaatatcgc
      361 ggaacctgag tcgcgcccat ggccggccgc acttcgagag tgttcccgtt cacgaggtcc
      421 caagcctggt tctgataagg gctcgtggaa gccgtactcc ccgccatgcg ccggaacatc
      481 gtcgagtcca ggtccgcctg gttccacgat ttgtaagtgc cgttcggagt cagcagctcg
      541 aggtgccgaa tgtaggcgat gttgtccgcc gtagagttct gcgcgggcgc aaacggattg
      601 tccggcaccg gctgccagcc ggtgaccggc tcgatgtaga ggagctgatt gaagtcaatc
      661 ggctcggcat tcacccgcgc caaaggtgtg gccggcacgg tgttcggagc gaaataaaga
      721 ttccagccga aagcgccagg aacaaatgta ggcgccgaga cctggcatag attgttcagc
      781 gccactacca cctgcacgcg cggcgtcacg ttggtttcgc tgccactctc tgtggcgagc
      841 gagtaagcca gatcgtaggt ccgtcccgcg agagcgccgc cggccacgct agcgcctact
      901 gcaccgacgg tggggtcggc gatactaacc agcgtgagcc gttccgccgc ggcgttcaga
      961 gtgatcgaga ccggtccgcc caaattcttg ctaatctgcg tggtgatcac ggccacccaa
     1021 agtgactcga cggcatcact caccgcctcg cccgccttgt tggtcatccg agagtcgaac
     1081 gtctccccca gccagtccga caacgatgct ttgaacccgc ccagcgtgtc agtggacaca
     1141 tatccctttt acgtgccccc tgttgttgta gcggcgatct ctgatcgccg aggcccttac
     1201 ttctttttct gcgcccgcgc aatcgcggcg atctgttccc gcatttcaga catctggttc
     1261 ttcagctccg cgttctctga agccagcgcc gaagcgctcg gagccttcga ctgatcgaac
     1321 cccaccggat tgatcccgtg gtccttgagc gagatcccca tttcctcgat gtaaagcgcg
     1381 ttgcgtcccg acggcgccac cgcttgcccg ccctgctgga tgcgcagctt ctgctcctgc
     1441 aggtattgct caaatctttg ctggcacgat gcggcgagca gttttcgcgc ttcttccttc
     1501 gcctcatcca ccaggtactc gacacccagc tggtttttca ttttgacggc cgtcacggcc
     1561 acgatcccgt ggatcggccc cttgtgccgc aaaataatct gcgccaccgg cacgcggtac
     1621 tcgatcgcgt cggaaaactc catcacctca tcgggcactt ctgtcgcctc ccccggaggt
     1681 atgcggtaaa tcacgttccc cacctgctct tcgacggttc cgaacttatg gtcttcgtgc
     1741 aacagcacta cgcttcctga catttctccc cctcctcgta aaatggcccg cagccggcgt
     1801 gacgtctgtc ttgccttcgg ccctgtgacg ccgcgtgatc tgatgggcgt agtctagaaa
     1861 cctctcgtcg tctccgtgcg cgatcaccgt ctcaccggtc aacatcgcgc gctccacttc
     1921 ggcgaattgc ttcacttgct tgctcgaaag cgagtcgatc gcttcctcaa gcttcgaatc
     1981 acccgccttg atattcgctt ccttcacttt cttcgccttc tcgcgcttct tttcgagata
     2041 ttcttcagtg ttgcgatatt tccgcatgtc gccggcaccg agcatctcga tcaccaactc
     2101 gatcgggata ggcatccggt cttcgtcctc cggccaggct gacacctgca gccagccgcc
     2161 ggcttccgga gccagccgtt caatcaccat accctcgatt cgaaactcgt aacgagctcg
     2221 cagcttcgag tcgtacgcct gcagtttgct gttaacttcc ttcggaacac gctccactcc
     2281 cgcgcggtcc gcctccagcc gctcgtccac ttgccgctgc agctcgattc ccgccgcttc
     2341 gagtggcgga gtcatccgca aaacatcgac cattccctgc gcttcagccg taagaaattc
     2401 cataaagtga gtagacggca aaccgtagac agcacacagg gggccctgtc tgccgtctac
     2461 tgtctaccgt ctacttctcc actcctttgc cgcccgtcac tttagaaagt aggcggagca
     2521 gtcaagaacg ttgtcggtac caaacaattc cgcatcacgc cggcgagcga gcgccgcatg
     2581 atcccgacat tgaagtacca cctgagcaac gcctgcaccg cgtctgcgat gccgttcacg
     2641 ccggaaactc gagtccaagt ccccgcctcg tcttccgcaa ggcttaaggg caaagcttcg
     2701 aatttcttga gtgtctccac cgcgaaggca aagatcctgt ccggacgagc gtctttgtcc
     2761 tcgatgatct ccaggcccgc gtattcgaac acgttgaagc cgaggtcgat tgcccgcttg
     2821 gtcgcgctgc cccgcgcgga agccgcgttc aggtcaaacc gaatggtgga tgtcgcgatc
     2881 gccaccagtt gattcacttg cgccgacggg aagatgaatg tatcgacatc cacgccgccc
     2941 cggtttttgg tgaactgccg gagttgttgg atgccctgtg aggtgatttg cacgttggcg
     3001 ttgtcaaaag cgaatgacgc ccacgcctgt tgccccgccg tggccggatt caggccttga
     3061 atggcgcccg tatccgagat cagcgagatc aggccttcac aggtcaacgg gaaatctccc
     3121 gcgaccagcg gagcctcgcc tgagagatag atcgcatgac ccacagtggc cgttccaccg
     3181 ttgtgggtga tcactcccgt cgcgtagttg atggcagaga tcaccgcgcc ttgcagtgac
     3241 aaagctccgc caccgacagg accgcagtcg ataggctgcc caaggcggag ataaagagcc
     3301 gccgccgaag ttggggaaag cgccgggacg gtaatcgagc ccgccgccga cgcgttcacc
     3361 gttccaagct gcccgccgcg atccgcgccc gccaccaaaa cgttcgcgtg cgagaccatg
     3421 tccagcaggt cgtatttcga ggtcaacgcc ttcgcttgca tgaaggctcg agcctcgcct
     3481 tttgacaggt tttcagcctg cccggtgatc aactgggggt cgtaatcgta tttcaggagc
     3541 acggtggagt tcgccaggcc ttggttgccc gcgctcccta cgcccgtggt gccttctttc
     3601 cggaacccga tgttccagtt tggctgcatc ctcgcgccaa acacgtaccc gcgaatgccg
     3661 atcaggttga tgggcttccc caccttcgat ccgtcgccga aaagattcat cagcgccggc
     3721 agcgtcgaga tctggtcggt catcgcgccg tatacatatt tcatgtcggc caggaaggtg
     3781 ttgctatcca cggcgcactg cgccaccggg ccgtcgtatt cgtagaactc ttgctcgatg
     3841 atctcgttcg agaccatatc catcactact ttggtgtaga cccgcatggg gcacactcct
     3901 cgttgattga attttgggtg ttcgttcaac gagggcgaaa cgtcgcgttc ttcagcccgt
     3961 tcgctctggc ggggagccct gctctgccct tgcggtgtcc cggaattcgg aatcctgaat
     4021 cccggaatct cttagttggc gggactaaac tgctctgccc ttgcggtgaa aatcactttc
     4081 agttgctcga ttgcgaaatc ttcaaactgg aaatagggaa actccactag cgcgcgctgc
     4141 ggccgtccag gtaccagcgc cgcgaggtcc acaatcgtgc caatccttcc atttacttca
     4201 actatgtcga gttctctcat tccacaaatc ctgcgagcat cagcgcctct tgccgttcgc
     4261 ggcgccactg ctcaacctgg ctcacgcata ctttttgtgg atcaaccccg gatcctcgat
     4321 caactcctgg agcgacggcc gtttgttccc attcgccggc ggcttcttct ctccgggctt
     4381 gtgctgcacg cgcgccgttt cccgagtgcc atgctcgccc accagcgcga gataatcggc
     4441 gttcgcttgc tcgaaatact tagccacgct tgaaactttt ccccggttca acccgtccag
     4501 cgcttgttgg tccttcgcga tcagctcgca agtgcgctgg tagacccact ccccaccttt
     4561 gtcccccggc cgcgtggctt cgtccggata cttcgcttcg atctgcttct tgaactcctg
     4621 gttgaaggtg aaattgcctt cgtcggcctt ccgggtcgct tccgcttccc gctgcttgcg
     4681 catgtgctca ttcagaggtt tcgtccattc ctccatctcc cgcttcacta attgacgcac
     4741 gtccgggccg cccgcctctt tttccttcct cagcgcttcc gatcgcaacc atgcgagcgt
     4801 ccccaccagg tcgtctttca aagcctgatc gatctgctcc agcgtaaacc cgagcgttgc
     4861 tcccagctcg tgcgcttccg tcgagatctt ctgataagcc gagtggccgg tggcaacctc
     4921 ttccggcttc gcgttaagtt tttcccaagg ctcgtacttc gccttcagtg cctcaaacgc
     4981 ctcacggaac tgccgcgtgt tggcggtttc caccggcgcc ggcgttcctg cggccggagt
     5041 tccggtaccg gcccccgcac cacctcccgg atcggcgcca gcagccggcg ctccgggagc
     5101 tgggaccggc tccgcgcccg ttcctggatc gacagcctgg ttgttcagtt cgtccgccat
     5161 ttacttcccc ctccggaatc cagaatcctg aatcctaaat cctttttaga gcgcttccag
     5221 cctggtcttc cccccacccg tcttcaccac actcacactg cccggttttg gtgcaccgct
     5281 cgtcgggata tcttcttccc cggcggagtg gacatgcgga agggcaagga gatctgttga
     5341 gtgcgtgaca gcaacgacat ggccgcccgg attcgctttc tgcttctccc gaagtattga
     5401 gaccccgtcc ttaagccgcg ccaggtagga attgaaggac tctcccctgg gaataggctc
     5461 atctggcttg tcgttctggt actcatcgag ctcacccttc acgtccttaa cattcttccc
     5521 ggtaaacttg ccctttttcc acggagctag cttgtccgta gt
//
LOCUS       DASYVO010000456        10659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_126595, whole genome shotgun sequence.
ACCESSION   DASYVO010000456 DASYVO010000000
VERSION     DASYVO010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 10659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10659
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126595"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VGR03_15565"
     CDS             <1..618
                     /locus_tag="VGR03_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995750.1"
                     /translation="AVSQKGGKPQKIVIVPKDKCGGGTNTLAVSPDEQRIAFAMDADG
                     DEQIAECSALWIADLPTGNLRRVPTTDLHPLSPFWLDNDTILFSGEKDNKPIGIYRLS
                     LSTGNLTHILDGHYLTPFVCDSGKTLYFSWGPNLQTKTPSGDAWPTFNDFSGFHIWRV
                     PLRDVLQQHIGVSDTVDPKTGDPTINYAFDAVAPTGCTPGTGCGS"
     gene            629..979
                     /locus_tag="VGR03_15570"
     CDS             629..979
                     /locus_tag="VGR03_15570"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1995751.1"
                     /translation="MKSDLTDCLTKAVKAVSEGKRFLTPKVSEIVLEGFLKTGSQHQQ
                     EGRAGPRTTPRELEILRLLAEGKSNKEIAALLGITVRTVETHRAKIMLKLGIHSLAEL
                     IHYALRHGIIARGI"
     gene            complement(1875..2876)
                     /locus_tag="VGR03_15575"
     CDS             complement(1875..2876)
                     /locus_tag="VGR03_15575"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEV1995752.1"
                     /translation="MSAKDKTKAGAPPPGHSKESDRQKEIDRLEKEVGELRHLAGDAD
                     ADAELEGIREQVAELRREFYTHLGPWQRAQIARHQQRPYTLDFIGMLFTDFMELHGDR
                     AYADDKAIITGFGKFHGRPVAIIGHQKGRDMKQRLVRNFGQPKPEGYRKALRVMQLAA
                     KFGRPILTFIDTPGAYPGIDAEERGQGEAIARNLREMARLPVPVIVTVTGEGGSGGAL
                     AIAVGDRVNILENSFYSVISPEGCASIIWRDSTKAETAAAAMKITAKDLKELGIVDEI
                     VPEPEGGAHNDHEAAARFLDEVLDRQLVELTNQPIRELLDARYEKFRKMGQFFDLGN"
     gene            complement(2873..6376)
                     /gene="dnaE"
                     /locus_tag="VGR03_15580"
     CDS             complement(2873..6376)
                     /gene="dnaE"
                     /locus_tag="VGR03_15580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV1995753.1"
                     /translation="MGQPQFVHLHVHTDFSLLDGACETSELLDEASRQKMPAVAITDH
                     GNLFAAANFFYEASKRDVKPIIGCEVYVAKGSRHDRGEKTHSGNGQDRGDAEPGMRGS
                     NHLVLLCESLEGYHNLIKLVSAGFLEGFYYRPRIDYDLLAKHSKGLIALSACLRGPVT
                     EAVVDQKYDQARENAYRLRDVFGKGNFFLEIQDQGLPIEKDVNPELVRLSKETGIPLV
                     ATNDCHYLHHDDAHAHEVLLCIQTGKTMSDTNRMKFQTDQFYFKSAVEMAQVFGEIPE
                     ALSRTVDIAARCNVKVERIPNPFPEFKVPEGHTSASYFESVVRQGFAARVPQLERQAQ
                     QGLLRNSLAEYEQRLTSEIEMIKKMRYEGYFLIVWDFIHYARAQDVPVGPGRGSAAGS
                     LVSYALRITDVDPLRYNLLFERFLNPERVSMPDIDIDFCMRRRGELIEYVTQKYGREN
                     VAQIITFGTMAAKAAVKDVGRAMDIPYGEVDRLAKLIPTTLGIELKDALEEAPQLKSA
                     INSDDRLKDLMAVALRLEGLSRHASTHAAGVVISSQPLTDIVPLYKSNRDEITTQYDM
                     NALERIGLLKMDFLGLTTLTVLHDTVHMIQQNRGAKIDIDNLALDDLETYKLFARGET
                     TAIFQFESHGMRDILRRYQPTRIEDLTALNALYRPGPIQGGMIDDFINRKQGKTKVSF
                     ELPQLKDILEETFGVILYQEQVMQIANRLASFSLGEADILRRAMGKKKKEEMAAQRAK
                     FMTGCTSNKIPEKKADRIFNLMEEFAGYGFNKSHSCAYALLAYQTAYLKTHYPVEFMA
                     ALLTSEAGNTEKVVKYINEARGMSISILPPDVNESDLYFTPVGESIRFGLAAIKNVGE
                     NTAKAIRESRLGQGEFKSLYDFCERIESRFLNKRVFESLIKSGAVDSLGPRESMLSSV
                     DDAVAALQRASRTRESGQHGLFGTAAAPAPMPFELREAAPWSEEERLASEYAMLGFYV
                     SGHPLAKYASRVMDLKTVSLDQIEGQRNGKDITVAALIVGTRPMRSKKGARWAIFTIQ
                     DMTGVQELLAFPESFARLESVLKPGNPLLLKVRVQVEEAGTRLSLQEARRLEEMAEGA
                     ASSEFRVRIEMKSLSEDVLDRLEDLFTAAPGPCQVVFELCSPDGSVAVLQAQQRVKNT
                     PELVEAVREICGNLAVRMVPE"
     gene            complement(6536..8281)
                     /locus_tag="VGR03_15585"
     CDS             complement(6536..8281)
                     /locus_tag="VGR03_15585"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEV1995754.1"
                     /translation="MSVSSPQLIRFGHFEVDTRSGELRRQGSKVNLQEQPFQALVLLL
                     ERPGEVVTREELNKRLWPENTFVDFERGLNKAINKLRAALRDNAEKPCFIETLPQHGY
                     RFIAPVENPPPVRDRLRPQHPPQIDSLAVLPLENLSGDPAQEYFSDGLTEELICAVAR
                     IASLRVISRTSVMPYKGARKSLPSIAKELRVDAIVEGTVARSDQKVRITAQLIYAPDD
                     RHLWSGRYERELHDILQLQAEIAADIASQIHKLVDPGIRPSVPVRKVHPQAFEACLKG
                     IFFRDKMTPADLSKSTAYFTQAISLDSAYAQAYANLSQAQFYLGLFGMGPASELFPKA
                     KASALRALELDETVASAHNALAAVHILYEWDWAGAEAECIRALQLSPNDSVTHVHLAD
                     YMSIQERHDEAIAEYKLALELDPMSCVYIGFFGLILYRARRFDEAIAQCHKALEINPN
                     YANALWFMALSLEQKGQLAESIEKFEKAFSLGAGPPCRALLGRAYALAGERTKAESIL
                     EELKTLSQQQYISPFDIAVVYAGLGDLTSTFQLLEEAYQQRVFRIIELTFPMFDNLRP
                     DPRWQDLVRRIGIPV"
     gene            8468..8947
                     /locus_tag="VGR03_15590"
     CDS             8468..8947
                     /locus_tag="VGR03_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995755.1"
                     /translation="MQRKSISRISMIATPVMIVCGLLASGAPARAQSEDDGPCSNQTL
                     RGDYGFAVEGLILPAPGVTIPIRGVHMTHFDGNAKLTQVDSIILNGSPISDGTPVTGT
                     YHVNANCTGTILLLPSTGGFVNLRIVVVRQGKEIHTVVWPPFDGPNRTVTSVGIKVE"
     gene            complement(9148..>10659)
                     /locus_tag="VGR03_15595"
     CDS             complement(9148..>10659)
                     /locus_tag="VGR03_15595"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995756.1"
                     /translation="YMFLLESRLSPEQRAVMLRVQELSAALGFNVYLTGGTVRDLVTG
                     ASLRDLDFTVEGNPSKIAHELEKGGAKILSEDEKLRHVEVLFAGDCEGSISAARDDHY
                     VRPGTRPEIRWSTIMEDLRRRDFSLNSIAISLNPASRGLLLDPTNGLSDIERAEVRAL
                     TIHSFTNQPVRLLRVLRFAARMGFKIEQRTQEWFDLAIERNLQETIAPEDAGGELRAV
                     AREERPAVVLKAWEEHDLLEIVNPVLAKKHPAYDAIGRLMKVREDLFTAGFRPRLAAP
                     MLVAVLGRLKDREQASVLSKAGFRSAEAEAALGFEEESWEAQKELVGRKMNAPIDAFR
                     FLEKLPLDQIGYLMAESNKSAALSKIRAYLNKWRPIRNALPVVGTELEKLGMPRGTKF
                     DQIVEQVFAMQLTGRGKTPEEREKILRKLSGIKEPPKKKEKEKKPSKGIDKAHAAAAM
                     GDAAHKKHVAAKIEATKHAAKGKPASGKPPAKHAAPASHGKRAASGKKSHARK"
BASE COUNT         2245 a         3123 c         2961 g         2330 t
ORIGIN      
        1 gcggtgtccc aaaagggcgg gaaaccgcaa aagatcgtta ttgtacccaa agataagtgc
       61 ggaggtggta ccaatactct ggcagtttcg ccagacgagc aaagaatcgc cttcgcaatg
      121 gacgccgacg gcgacgaaca gatcgccgag tgcagcgctc tgtggatcgc cgatcttccc
      181 acagggaatc tgcggagggt tccaaccacc gatctgcacc cgctcagccc cttctggctg
      241 gacaacgaca cgatcctctt ttcgggcgaa aaagacaata agccgatagg catttacaga
      301 ctgtccttga gcacggggaa tctaacgcac atcctcgatg ggcattacct gacgccgttc
      361 gtctgcgatt ccggcaagac gctgtatttt tcctggggac cgaatctcca aactaagacg
      421 cccagcgggg atgcttggcc cacgtttaac gacttctccg ggttccatat ctggagagta
      481 ccgttgcgcg atgtgctgca gcagcacatc ggcgtgagtg acaccgtgga cccgaagacc
      541 ggcgatccca ccatcaatta cgcgttcgat gccgtcgcgc ccaccggatg cacacctggc
      601 actggatgcg ggagctagcg ggtacctttt gaaatccgat ctcacggact gcctcacgaa
      661 agctgtgaaa gctgtttctg agggcaagcg attcctcact cccaaagtat ccgaaatcgt
      721 gctggaagga tttctcaaga ccggaagcca gcatcagcaa gaggggcggg caggtccccg
      781 aacaacccct cgcgagctcg aaatcctccg tctcctggcc gaggggaaat ccaataagga
      841 gatcgcagcg cttttaggaa tcaccgtcag gaccgttgag acacaccgag ctaagatcat
      901 gctcaagctt ggcatccatt ctctggctga actgattcac tatgcattac gccacgggat
      961 aatcgctcga ggaatttaag cctttgactc tgccatattg ttgctcgtct cgcctccgca
     1021 gaagccgctc aacccgcccc cgccgccgtt caaccctcaa tttcgaccat tcaccgcaaa
     1081 acacttaaca acttgattag gccgtgctat tctctcccgt gtataccgaa tccatttcgc
     1141 gcccgctccg ctttcccccg attcgtccgc ctgtctctct ctcccgacgt ttcgaccttc
     1201 ggacgtgcca tcgttcagac gtcgtttcgg ttattcccct gccagctgcc ctttcttttc
     1261 aacgtttgga ccttcagacg tcccaacgtt cagactccgt tcctgccccc ctgccccact
     1321 atccccctaa ctcctttaca atccgcagat ccaagacccc cctcccgcaa gtcctttata
     1381 atccgcactt acagacaccc tccgcaagtg cggaaaacaa agcacttata acccctttag
     1441 aatccgcact tacagaaaat gcgcccgtaa ctcctttaga atccgcactt ccaaaaacac
     1501 gggggtgcgg gcacccaatc gccctggcac cccttgttca catcccatca ctaagtcctt
     1561 tagttaccac acttctggag tccttcctaa gtgtggcaaa caaagaactt acacaaaacc
     1621 taagtccttt agttgccaca cttacaaaaa acagggggtg gggggtgtta ttgttaacta
     1681 gaaatcccac gaaggatttc tatcccgaga gaccatcggg agtcgaggga tctcgcctca
     1741 ttcccgataa gggaatctgt cctgagggag cccgccacgg cgtgcgacga aagatttctg
     1801 tcccgagtca ggatcgcgac cgagagcgcg ctctggcgtc ccaccagtca agagtcacga
     1861 atcacgtgtt ttcttcaatt gcccaaatcg aaaaactgcc ccatcttgcg aaacttctca
     1921 tagcgcgcat ccagcaattc ccggatcggc tgattcgtca gctccaccaa ttgccggtcc
     1981 agcacttcat ccaagaaccg cgccgccgct tcgtggtcgt tgtgtgcgcc gccttccggc
     2041 tccggcacaa tttcgtcaac gatgcccagc tccttcaaat ccttcgccgt aatcttcatg
     2101 gctgcggccg ctgtttcagc cttcgtggaa tcgcgccaaa taatcgaagc gcatccctcc
     2161 ggcgaaatca ccgagtaaaa gctgttctcc agaatgttga cgcgatcgcc gacggcaatg
     2221 gccagcgctc cgcccgaacc gccctcgccc gtcaccgtga cgatcaccgg caccggcagc
     2281 cgcgccattt cgcgaagatt tcgcgcaatc gcttcgccct gaccgcgctc ctccgcatcg
     2341 atccccggat acgcccccgg cgtatcgata aacgtaagaa taggccgtcc aaattttgcc
     2401 gcaagttgca tcacgcgtag cgccttgcgg tagccctcgg gcttgggctg cccgaaattc
     2461 cgcaccagcc gctgcttcat gtcgcggcct ttttgatggc caatgatggc cacggggcgc
     2521 ccatgaaatt ttccaaaccc cgtgatgatc gccttgtcgt cggcataggc gcggtcgccg
     2581 tgcagctcca taaagtccgt gaaaagcata ccgataaaat ccagcgtgta ggggcgctgc
     2641 tggtgacgcg cgatctgcgc gcgctgccac ggccccaggt gggtgtaaaa ttcgcgccgt
     2701 aattccgcaa cctgctcgcg gatgccctcc agttccgcgt cggcatccgc gtccccggcc
     2761 aggtggcgca attcgccgac ctctttttcc aggcggtcga tttctttttg gcggtcgctc
     2821 tctttcgaat gccctggagg aggcgcgccg gctttagttt tatcttttgc gctcattcgg
     2881 gcaccattcg aacggcgagg ttgccacaaa tttcccggac tgcttccacc aattcgggcg
     2941 tgtttttcac gcgctgctgc gcctgcaaca cggccacgga accgtccggc gagcacaatt
     3001 caaacacgac ctggcaaggt ccgggcgcag cggtaaacaa atcttccaaa cggtcgagta
     3061 catcttcgct tagcgatttc atttcgattc gaacgcgaaa ctcgctggac gccgccccct
     3121 ccgccatctc ctcgagccga cgagcttcct gcaaggaaag ccgcgtgccc gcttcctcca
     3181 cctgcacgcg aactttcaag agaagcgggt tgcccggctt caagaccgat tcgagacgcg
     3241 caaagctttc aggaaaagcg aggagttcct ggacgccagt catatcttga atcgtgaaga
     3301 tggcccagcg cgcgcctttt ttcgaacgca tggggcgcgt accgacaatc aacgcagcaa
     3361 cggtgatatc ttttccgttt cgctggccct ctatctgatc gagtgaaacc gttttcagat
     3421 ccatcacacg ggaagcgtat ttcgcaagtg gatggccgga aacgtaaaaa ccgagcatgg
     3481 cgtattcgct ggcgaggcgt tcttcctcgc tccagggcgc ggcctcgcgc aattcgaacg
     3541 gcatgggcgc gggagcggcg gcggttccga acaatccgtg ctggccggat tcgcgcgtgc
     3601 gggaagcgcg ctgcagcgcg gcgacggcat cgtccacaga cgagagcatg gattcacgcg
     3661 gaccgagcga atccacagcg cccgacttaa tcaggctttc gaacacacgt ttgtttagga
     3721 aacgcgactc gatacgctcg cagaaatcat aaagggattt gaattctccc tgaccgagcc
     3781 gcgattcccg aatcgctttg gcggtgtttt cgccaacatt cttgatcgcg gcaagaccga
     3841 agcgaatcga ttccccgacg ggggtgaagt agagatcgct ctcgttcaca tcgggaggaa
     3901 gaatagaaat actcatgccg cgcgcttcat tgatgtactt cacgactttc tcggtgttgc
     3961 cggcttccga ggtcaggagc gcggccatga attcaacggg ataatgtgtt ttcaggtacg
     4021 ccgtctgata agcgagcaaa gcgtaggcgc aggagtgaga cttgttgaag ccgtagccgg
     4081 caaactcctc catcaagttg aagatgcggt cggctttttt ctccgggatt ttgttggaag
     4141 tgcacccggt catgaatttg gcgcgctgcg cggccatctc ttccttcttt ttctttccca
     4201 tcgcacggcg aagaatgtcc gcttccccaa gtgaaaagct ggccaagcga ttggcgattt
     4261 gcatcacctg ttcctggtag aggatgacgc cgaaagtttc ctcaagaatg tctttcaact
     4321 gcggaagttc gaagctgacc ttagtcttgc cttgtttgcg gttgatgaaa tcgtcgatca
     4381 tgccgccctg gatgggaccg ggacggtaca acgcgttgag cgcggtgaga tcctcgatgc
     4441 gggtcggctg gtaacggcgg agaatgtcgc gcattccgtg agattcaaac tggaagatgg
     4501 cggtcgtttc tccacgcgca aaaagtttgt aggtttcgag gtcgtccaag gcgagattat
     4561 cgatgtcaat tttcgcgccg cgattctgct ggatcatgtg aacagtgtcg tggagtacgg
     4621 tgagcgtcgt caggccaaga aaatccattt tcagaaggcc gatgcgctca agcgcgttca
     4681 tgtcgtattg cgtggtgatt tcatcgcggt tggacttgta gagcggaacg atgtccgtga
     4741 gcggctgcga cgaaatcaca acgcccgccg cgtgagtaga agcgtgccgg gacaatcctt
     4801 cgaggcgcaa cgccacggcc atcaaatcct tgagccgatc atcactattg atggcggatt
     4861 tgagctgcgg ggcttcttcc agagcatctt tcaactcgat gccgagcgtg gtgggaatga
     4921 gcttggcaag acggtccact tcaccataag gaatgtccat ggcgcggcca acgtccttga
     4981 ctgcggcttt cgcggccatg gttccgaacg taatgatttg cgcgacattt tcacggccgt
     5041 atttttgcgt cacgtattcg atcagctcgc cgcgccgccg catgcagaag tcgatgtcga
     5101 tatcgggcat ggagacgcgc tcggggttca ggaagcgctc gaagagcagg ttgtaccgca
     5161 aggggtcgac atcggtgatg cgcagggcgt agctcaccag gctgcccgct gccgaaccgc
     5221 gccctggtcc gacaggaaca tcctgcgcgc gcgcatagtg gataaaatcc cagacgatca
     5281 agaagtagcc ctcgtagcgc atcttcttga tcatttcgat ttcggaggtc agccgctgct
     5341 cgtactcggc cagtgaattc cgcagcaatc cttgctgcgc ctggcgctcg agctgcggaa
     5401 cgcgagcggc gaatccctgg cgtaccacgg attcaaaata gcttgcggag gtatgccctt
     5461 ccgggacttt gaactccggg aaaggattgg ggatgcgctc gactttgacg ttgcagcgcg
     5521 cagcgatgtc cacggtacgg cttagcgctt cgggaatctc gccaaaaacc tgggccatct
     5581 ccaccgcgct cttgaaatag aactggtcgg tctggaactt catgcgattg gtgtcgctca
     5641 tggtcttgcc ggtctggatg cagagcagca cttcatgggc gtgagcgtcg tcgtgatgga
     5701 ggtaatgaca atcgttggtg gcgacgagcg ggataccggt ctctttcgaa agacggacca
     5761 gttcggggtt gacatctttt tcgatcggca atccttgatc ctggatttcc aggaagaagt
     5821 tccctttgcc gaaaacatct cgcaagcggt aagcattctc gcgcgcctgg tcgtacttct
     5881 gatccacgac cgcttccgtc accgggccac gaagacaggc cgagagcgcg atcaagcctt
     5941 tgctgtgctt ggcaagcaaa tcgtagtcaa tgcgagggcg atagtagaag ccctcgagga
     6001 aaccggcgga gaccagcttg attaaattgt gatagccctc gagcgattcg cacaggagca
     6061 cgaggtgatt cgagccacgc attccgggct cggcgtcgcc gcgatcctgg ccgttgccgc
     6121 tatgtgtttt ttcgccgcga tcatggcggc tgcccttggc cacgtagact tcgcagccga
     6181 tgatgggctt gacgtcgcgt ttgctggctt catagaaaaa attagcggcg gcaaacaaat
     6241 tgccgtggtc cgtgatggct acagcgggca tcttctggcg ggacgcttca tccaaaagct
     6301 cggaggtttc gcaggccccg tcgagcaggc tgaaatccgt gtgaacgtga agatgaacga
     6361 actgcggctg gcccatggaa ctcgattcta acaggatacg gaaaaagtcg atatgctccg
     6421 gaccatggcg gcgcgtaacg aagcaggcca gcctaagtag ttaggtccaa gtctctatct
     6481 tagaaatgct ggctgcaatg ctagctctcg gttcccgaat cactcggatg ctgccttata
     6541 ccgggatgcc gatacgccgc acaagatcct gccagcgcgg atcgggtcgc aggttgtcaa
     6601 acatcggaaa cgtcagctct atgatccgga agacccgttg ttgataggcc tcctccagca
     6661 gttggaatgt ggaagtcagg tcaccgaggc cggcgtagac gacggcaata tcaaatgggg
     6721 agatgtactg ttgctgggac aacgtcttca gttcctcaag gatgctttcc gccttcgtcc
     6781 gctcacctgc cagtgcatag gcgcgtccca gcaacgcccg gcaaggaggt ccggcgccaa
     6841 ggctgaatgc cttttcgaat ttttcgatcg attctgcaag ctggcccttc tgctccaagg
     6901 aaagcgccat aaaccacaga gcatttgcgt agttgggatt aatttccaaa gccttatggc
     6961 attgcgcgat cgcttcgtca aaccgccggg cccgatacag gatcaaaccg aaaaagccta
     7021 tatacacgca agacatcgga tccagctcga gcgccagttt gtattccgcg atggcctcgt
     7081 cgtgccgctc ctgaatggac atgtagtcgg ccaaatgcac atgcgtcacc gaatcattcg
     7141 ggctcaactg cagggcgcgt atgcattcgg cttccgcccc ggcccaatcc cactcataaa
     7201 ggatgtggac agcggccaac gcattgtgag cggaggcaac ggtttcgtcc aattccagag
     7261 ctctcagggc gctcgccttc gctttgggga acagctcact ggcaggcccc atgccgaaaa
     7321 gccccagata gaattgggcc tgcgagaggt tcgcgtaagc ttgagcgtag gcagagtcaa
     7381 gactaatcgc ctgggtgaaa taggcagtgc tctttgacaa atccgccggg gtcatcttgt
     7441 cccggaagaa gattcctttg agacaagcct cgaaagcctg cggatggacc ttgcgcaccg
     7501 gaaccgaggg acgaattccc gggtctacca gcttatgaat ctgactggcg atgtctgcag
     7561 caatctcggc ctgtaattgc aggatgtcat gcagttcgcg ctcgtatctt ccggaccaca
     7621 ggtgtctgtc gtctggagca tatatcaact gagctgtgat tcggactttc tggtccgacc
     7681 gtgctacggt gccttcgaca attgcgtcta cccgcaattc cttggcaatc gacggcaagg
     7741 acttgcgcgc acctttgtag ggcatcaccg aagtccgcga gattacccgc agggaggcga
     7801 tgcgggccac ggcgcaaatc aattcctccg tcaatccgtc ggagaaatat tcctgcgcgg
     7861 gatcaccaga caggttctcg agcggtaaca ccgccaggga gtcaatttgc gggggatgtt
     7921 gcggccgtaa gcgatcgcgc actggcggag ggttttcaac gggggcaata aagcgatatc
     7981 cgtgctgtgg cagggtctcg atgaaacacg gtttctcggc gttgtccctc agagctgcgc
     8041 gtagtttatt aatggctttg ttcaggccgc gctcgaaatc gacgaaggtg ttctccggcc
     8101 acagtctttt gttgagttct tcccgcgtca ccacctcgcc gggacgttcc agtaacagca
     8161 cgagcgcctg aaacggctgt tcctgcagat tgaccttgga gccttgccgg cgcaactcgc
     8221 cagaccgagt gtcaacctcg aagtgcccga aacggatcag ttgcggcgaa gaaacgctca
     8281 tttggtgggt cgcagtataa cacctggcct ttcatttccc gcgatcggct tccttatcct
     8341 gcggtaccct tctgattcta agatgtttac cagggaacga atggggaaca aagagggcac
     8401 gcagcggcca ttgcaacccg cgcgtctgaa ccgtattgtc ggcccggacc taaaaggagg
     8461 tctcaatatg caaaggaaat caatttcgag aatctcgatg atcgcgactc ccgtgatgat
     8521 cgtttgcggt ttactggcgt caggcgcgcc tgccagagcc cagtcggagg acgacggacc
     8581 ctgctcgaat caaaccctgc ggggcgatta cggattcgcc gtcgaaggcc tgatcctccc
     8641 cgcacccggc gttaccatcc cgatccgcgg cgtgcacatg actcactttg acggcaacgc
     8701 taagctgacc caggttgact ccatcattct caatggcagc ccgataagcg acgggacccc
     8761 tgtcactggc acctatcacg ttaatgccaa ttgcaccgga acaattcttc tccttccgag
     8821 caccggtggg tttgtaaacc tgcgcatcgt ggtggtcaga caaggcaagg aaatccacac
     8881 cgtggtttgg cctccgtttg atggcccgaa ccgcactgtc accagcgtcg gcataaaagt
     8941 cgagtaatcg ctgctgtgta gaccagttgt gatggcaagg aagcaccggc tgcaacttga
     9001 atgaagaaag cggaacccat gttcttagag ggctgtttga ctgttcgaac gggccggggc
     9061 gtcctcacca acaagtacgt cccggcgtat cccttccgcc tgaatagtac cttcccgccg
     9121 cttggacgat aactcggaac tagcgcgcta cttgcgggcg tgactcttct tgccggaggc
     9181 agcacgcttg ccatgggatg ccggggcggc gtgcttcgca gggggcttcc cggaggcagg
     9241 tttccccttg gccgcgtgtt tcgtcgcctc gatttttgcg gccacgtgct tcttatgagc
     9301 ggcgtcgccc atcgccgcag cggcgtgtgc cttgtcgatg cccttcgacg gcttcttttc
     9361 cttctctttc tttttcggcg gttccttgat cccgctgagc ttcctcagaa ttttctcgcg
     9421 ctcttcaggg gttttcccac ggccggtgag ttgcatggcg aagacttgtt cgacaatctg
     9481 gtcgaacttt gttccacgcg gcataccaag tttttccagc tcggtgccca caaccggaag
     9541 agcgttgcga atcggccgcc acttgttcag ataggcccga atcttcgaaa gggccgccga
     9601 cttattggac tcggccatca ggtagcctat ctgatcgagc ggaagttttt cgaggaaccg
     9661 gaaagcatcg ataggagcat tcatcttgcg cccaacgagc tccttctggg cctcccatga
     9721 ttcttcctca aaaccgagag cggcttccgc ttcggccgaa cggaatccgg ccttggagag
     9781 aacgctggcc tgttcgcggt ccttcaagcg tcccagaacg gcgacgagca tcggagcggc
     9841 gagacgcggg cggaaacccg cggtgaacag atcctcgcgc accttcataa ggcggccgat
     9901 ggcgtcgtaa gccggatgtt ttttcgccag cacgggattg acgatttcga gaaggtcgtg
     9961 ctcttcccac gccttcagca cgacggccgg gcgttcctcg cgggcgacgg cgcgaagttc
    10021 gccaccggca tcttccggcg caatggtttc ctgcagattg cgctcgatgg ccaggtcaaa
    10081 ccattcctga gtgcgctgct caattttgaa gcccatgcgc gccgcgaaac gcaagacacg
    10141 caacaatcgg accggctggt tcgtaaagct gtgaatggtg agcgcccgaa cttcggcgcg
    10201 ctcgatatcg gacaggccgt ttgtgggatc gagcagcaag ccgcgcgagg cggggttcag
    10261 tgaaatggcg atggagttga gggagaaatc gcggcggcgc agatcttcca tgatggtaga
    10321 ccagcgaatc tcgggtcgcg ttcccgggcg aacataatgg tcgtcgcggg cggcggaaat
    10381 gctcccctcg cagtcgccgg cgaagagcac ttcgacgtgg cgaagctttt catcctcgga
    10441 gaggattttg gcgccgccct tttcgagttc gtgagcgatc ttcgagggat tgccctcgac
    10501 ggtgaaatca agatcgcgca gcgaagcacc ggtaacaagg tcgcgaaccg ttccgcccgt
    10561 cagatagacg ttgaatccga gtgcggcgga gagttcctgc acgcgcaaca tcacggcgcg
    10621 ttgttccggc gagagccggc tttccagcaa aaacatata
//
LOCUS       DASYVO010000457         4375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_137998, whole genome shotgun sequence.
ACCESSION   DASYVO010000457 DASYVO010000000
VERSION     DASYVO010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137998"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VGR03_15600"
     CDS             complement(<1..476)
                     /locus_tag="VGR03_15600"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV1995757.1"
                     /translation="MPLFQAIVLALVQALTEFLPISSTAHLILFPWLLHWQEPGLAFD
                     VALHAGTLLSVLLYFVRDWLTLALCGFGLKYPASATVEQVATHRRMFWYMVAATIPAA
                     VAGKLFHHQIEDTLRKPVIIGCSLVIVGLLMWWADARAKLSRNLEQSDFGDAASIGT"
     gene            complement(482..973)
                     /locus_tag="VGR03_15605"
     CDS             complement(482..973)
                     /locus_tag="VGR03_15605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEV1995758.1"
                     /translation="MAVLIRPYETRDFAALYRLDQSCFPPGISYTKTGLRYFLGMDGA
                     QCLVAEDGKAIAGFLLAEENRPLAHIITLDVAESHRRHGIGSALLRQTEANLAAREVR
                     AILLETATSNAAAIAFWQAHGYRSEAVLKRYYLGRLDAYEMRKTFPALSGSGGAGNKL
                     QEK"
     gene            complement(1175..2650)
                     /locus_tag="VGR03_15610"
     CDS             complement(1175..2650)
                     /locus_tag="VGR03_15610"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV1995759.1"
                     /translation="MATAITAVEPTVRVRATKLLINNEWVDSVSGKTFPTINPSTGEE
                     ICRVAEADAPDVDRAVRAARAAFETGKWKKMSAAQRGLLMNRLADLIEKNAEELAQLE
                     TLDNGKPYHVALGGDLPLTIACYRYYAGWADKLQGKTIPVSGKYFCYTKHEPVGVVGQ
                     IIPWNFPLLMQAWKLGPALACGCTVVLKPAEQTPLTALRVGELMIEAGFPPGVVNLLP
                     GYGPTAGAAIARHMDVDKVAFTGSTEVGHLIMKAAAETNLKRITLELGGKSPNIVFAD
                     ADMDQAVEGAHFALFFNQGQCCCAGSRLYVEDKCYDEFVEKSVARAKKRTVGNPFDKK
                     TEQGPQVDSDQFNKVMGYIEAGKKEKAMLLTGGKRVGDKGYFIEPTVFGDVQDDMKIA
                     QEEIFGPVMSILRFKDLDEVVARANKSLYGLAAAVWTRDITKAHAIADSVRAGTVWVN
                     CYDVFDAGAPFGGFKQSGMGRECGEYGLANYTEVKTVTVKL"
     gene            3459..4238
                     /locus_tag="VGR03_15615"
     CDS             3459..4238
                     /locus_tag="VGR03_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995760.1"
                     /translation="MLADLKDRVSLWLIHKQLAALEGRLFNCIVTLGHHKSLSDAKSA
                     QLHLVLKAASAARAAIVLLARDFRQEAEAPQRAFLEAWVYAMHFTWFPGSAAFDEWQR
                     RPDRPLDTKTFRLRDEVELEVNRRLDLSIAQQFPVRTLFRIFSNISIHPTRDTVDRAW
                     EEVAGRRRFRRSDPDSQLMYGSLERIKGITKTATTFVQLHLFYDFLRKYVLSGADVPR
                     RFVLPKTIFAESLIRHWLREFSPYIEFAFRVADEGYGRRRP"
BASE COUNT          927 a         1180 c         1408 g          860 t
ORIGIN      
        1 gttccgatgg atgccgcgtc gccgaagtcg gactgctcga ggtttcgcga aagcttggcg
       61 cgcgcatcgg cccaccacat cagcagcccc acaatcacca gggaacatcc gatgatcacg
      121 ggctttcgaa gcgtgtcttc gatctggtga tggaatagtt tgcccgcaac cgccgcggga
      181 atcgtagccg ccaccatgta ccagaacatt ctccggtgcg ttgcaacctg ttcgactgtg
      241 gcgctcgccg gatacttcaa gccgaaaccg cacagagcca gcgtgagcca atcgcgaaca
      301 aaatagagca gcacggagag cagcgtgccc gcgtgcaggg cgacgtcaaa agccagaccc
      361 ggttcctgcc agtgcagcag ccacgggaaa agaatcaggt gcgcggtgct gctgatgggc
      421 aaaaattccg tgagggcttg cacgagggcg aggacgatcg cctgaaacag gggcattgaa
      481 attatttctc ctgtaatttg tttcccgcgc cgcccgaacc gctcagcgca ggaaaagtct
      541 tgcgcatttc gtaggcgtcg agccgcccca ggtagtagcg tttgagcacg gcttcgctac
      601 gatagccgtg cgcctgccag aaggcaattg ccgcggcgtt gctggttgcc gtttcgagca
      661 ggatggcgcg cacttcgcgc gcggcgagat ttgcttccgt ctgccgcagc agcgcgctgc
      721 cgatgccgtg cctgcggtgg gattccgcca cgtccagcgt gatgatgtgc gccagcgggc
      781 ggttctcctc ggccaggagg aatccggcga tcgccttgcc atcttctgcg accaaacatt
      841 gcgctccgtc catgccgaga aaatagcgca gcccggtttt cgtgtaggaa atccccggcg
      901 gaaaacaaga ctggtcaagg cggtagagcg ccgcgaagtc gcgcgtttcg taggggcgga
      961 tgaggacggc catgggccaa acggcggaaa gagatttaac acagaaggcg cgcagaacac
     1021 agaggaggcg aaaaacaaag agcgtgcgcg aaacaaagag ggcactgcga ggcgcgaggg
     1081 gcggggatgg agaggaaaaa atgaggcaaa ggaaggcgaa ggcccggcgg agtgcccggg
     1141 ccttctgtta tcgagagtgg gtttgtctta acgatcacaa tttcacggtg acggttttga
     1201 cttcggtata gttcgcgagg ccgtattcgc cgcattcgcg gcccatgccg gactgcttga
     1261 acccgccgaa gggcgctccg gcgtcgaaga cgtcgtagca gttgacccag acggtgccgg
     1321 cgcgaacgct gtcggcgatg gcgtgggctt tggtgatgtc gcgggtccag acggcggcgg
     1381 cgaggccata gaggctctta ttggcgcggg cgacgacttc atcgaggtcc ttgaaacgca
     1441 ggatggacat gaccgggccg aaaatctcct cctgcgcgat cttcatgtcg tcctgcacgt
     1501 cgccaaagac ggtcggctcg atgaagtagc ccttgtcgcc gacgcgcttc ccgccggtga
     1561 gaagcatggc cttctccttc ttgccggcct cgatgtagcc catcactttg ttgaactggt
     1621 cgctgtctac ctgcggcccc tgctcggttt ttttgtcgaa ggggttgccg acggtgcgtt
     1681 tcttcgcgcg cgcgacgctc ttctcgacga attcgtcgta gcacttgtct tccacataaa
     1741 gccgcgaacc ggcgcagcag cattggccct ggttgaagaa gagcgcgaaa tgcgcgccct
     1801 caaccgcctg gtccatgtcg gcgtcagcga agacgatgtt gggactcttg ccgcccagct
     1861 cgagcgtgat gcgcttcagg ttcgtttcgg cagcggcttt catgatgagg tggccgactt
     1921 cggtcgagcc ggtgaaggcc actttgtcaa cgtccatgtg acgagcgatg gcggcgccgg
     1981 cggtggggcc gtaaccgggc aaaagattga cgacgcccgg agggaagccg gcctcgatca
     2041 tgagctcgcc gacacgcagc gcggtgagcg gcgtctgctc ggcgggcttc agtacgacgg
     2101 tgcagccgca agcgagcgcc ggcccgagct tccacgcctg catcaggagc gggaagttcc
     2161 acgggataat ctggccgacg acgccgaccg gctcgtgctt ggtgtaacag aaatatttgc
     2221 cgctgacggg gatggtcttg ccctggagct tgtcggccca gccggcatag tagcggtagc
     2281 aggcgatggt gaggggcagg tcgccgccga gcgcgacgtg gtagggcttg ccgttgtcca
     2341 gcgtttcgag ctgggccagc tcctcggcgt ttttttcgat gaggtcggcg aggcggttca
     2401 tcagcaggcc gcgctgtgct gcgctcatct tcttccactt gccggtctca aaggccgcgc
     2461 gggcagcgcg gacggcgcgg tccacatcgg gagcgtcggc ttcggcgacg cggcagattt
     2521 cttcgccggt cgagggattg atcgtgggga acgtcttgcc ggagacgctg tccacccact
     2581 cattgttgat gagcagcttt gttgccctga ctcttacggt gggctctacc gcggtgattg
     2641 ccgttgccat gaaaacctcc ttgaaaaagc atgcgccggg cctctccgcg cgagcagggt
     2701 gaaggcgggc gcggaagcgt ccttggggac gactccgcgg ggcgcaaacg atgcaaccag
     2761 tggaatcgta tacccctgag agaaaggaag caaggaagca gggaagtgcg gatcgcgcgg
     2821 cagggtattt ggaagaagcg taccaccccc caccccctgt tgtttttgca agagtgcgga
     2881 agcgaaagaa agaaaagggg ttgcggcgat tcctgcgcac ggcaagagtg aaaagagtgt
     2941 ggaagagctt gaaaggaaag gagatgaatg gtggttgatg accggtgatt cgtgagtggt
     3001 gacttggacg ggtgatccat gacggggaat tggtgacttg caggcgttgg gtgagatcac
     3061 ggctaggaga tttccaccgg aagtatcccc ccacccccgg gatgttttgc gtaagagtgg
     3121 caaacaaagg ggttacaggc gaagagtgaa aaagagtgcg caagggaatg aaaaaacaga
     3181 ggttagaagt tcgaggtggg aggttagaag gaatcggggc gtgcagggcg aacttcatgt
     3241 ggaaggatag cacggatttt gtaccgtgtc aataaagtac ttgcgggtaa tagggtcgga
     3301 atcgaccaga tgttagaggt tcgaggttag agattagaat gagggggagg agacagcagt
     3361 ttccggccga agtcaacgcc aaccaagggc ggggtacccg gattcgtatc actcctctaa
     3421 gtttaggcca cccccctgtg tattatgctg tttgactaat gcttgctgac ctaaaagacc
     3481 gcgtcagtct gtggctcatt cataagcaac tcgccgcgct cgaagggcga cttttcaatt
     3541 gcatcgtcac gctaggacac cacaaatcgc tatctgacgc gaagagtgcg cagcttcacc
     3601 tagtcctgaa ggcggcatct gctgcccgcg ccgcgattgt tctgctagca agagacttca
     3661 ggcaagaggc ggaagcgcct caacgagcct ttctggaggc gtgggtttat gccatgcatt
     3721 ttacgtggtt tcccggcagt gccgccttcg acgaatggca acgcagaccc gacaggccac
     3781 tcgacacaaa gacgtttcgg ctgagggatg aagttgagtt ggaggtgaac cgcagactcg
     3841 acttgagcat tgcacagcaa tttccggtca gaactttgtt caggatattc agcaatattt
     3901 caattcaccc aacacgcgat acagtcgatc gcgcttggga agaagtcgcc ggaagacgtc
     3961 ggtttcgtcg ctcggacccc gactctcagt tgatgtacgg atctcttgaa cgcatcaaag
     4021 gaatcacgaa aacagcaacc acattcgttc agttgcacct cttttatgac ttcctgcgga
     4081 agtatgtatt gagtggcgcc gatgtgccga ggcggttcgt gctccccaag accatcttcg
     4141 ctgaatccct cattagacat tggctgcgag aattcagccc atacatcgaa ttcgccttcc
     4201 gagtagctga cgagggctat ggccgaagga gaccatagat gtgtcctttc gcccttgcga
     4261 aagatgaagc gcgcggcgag tctactcggt cggaagagcc gaagcggtca tcgaatcacc
     4321 tgaagaagaa tccccacact ccccggcgcg gccggggtaa acgccagccg agggc
//
LOCUS       DASYVO010000458        14958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_41404, whole genome shotgun sequence.
ACCESSION   DASYVO010000458 DASYVO010000000
VERSION     DASYVO010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 14958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14958
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41404"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VGR03_15620"
     CDS             <1..654
                     /locus_tag="VGR03_15620"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEV1995761.1"
                     /translation="IPRAEMLFIVERIARALAVPVTADMEAGYSTTPEGAAETAQAVI
                     AAGAVGMNFEDSTGNAQAPLFDLSLQVERIKAIRLACASSGVPLVLNARTDVFLEQVG
                     DPPSRFDHAVRRANAYREAGADSLFVPGVSDRDVIARLVREIHGPVNILAVAGSPSVP
                     ELLRLGVSRVSVGSGPHRATMALTRRIATELLATGRYSSFTQDTITYAEANELFKRA"
     gene            843..1517
                     /locus_tag="VGR03_15625"
     CDS             843..1517
                     /locus_tag="VGR03_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995762.1"
                     /translation="MNIRAITAKFAEAGKPFLALWGAVRWIGQRFWTWELVSWLVGVV
                     FVGLAFVLAQYGWYLSGRLFLVLGAILLILRIVHDAMKSKDISVAEKIVASIIVTAVL
                     SGICAGGSAFINDIEWKNEIRIHLSFKSSPLFTKQREKKIIWELNSYYRYLTELGLDL
                     SPEIPPLGITPPHGPMSAGGQPNAPFYYSSLYISEDVIDNPDMVKAMYSTYIFGRLVR
                     DSTNTT"
     gene            complement(1734..2812)
                     /locus_tag="VGR03_15630"
                     /pseudo
     CDS             complement(1734..2812)
                     /locus_tag="VGR03_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611840.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3513..4505)
                     /locus_tag="VGR03_15635"
     CDS             complement(3513..4505)
                     /locus_tag="VGR03_15635"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEV1995763.1"
                     /translation="MAETTPFDTLMQISSAYALSRCLHVVADLGVADALDDSPRTAAA
                     LAEATGTDAGALGRVLRLLSAHGIFEGRDDGYVHTPASRLLRTDHPQSMRAFVRMIGL
                     PSFWASYQSLEHTLRAGRPAMEKVVPGGVWSYFVEHPEAGRIFDQAMTGKAQGQIGGV
                     VGGYPFSGFGLIGDIGGGRGHLLQAVLAATPTAKGVLFDLPQVIERASSEASDRLRLQ
                     AGDFFNDTMPACDAYLLMDVIHDWGDDESIRILKNIRRAAPAHAKLLLIEAIIPDDPH
                     PNFAKVLDIHMMTLLTGRQRTRSEHAKLLAGAGFRLERDIDVGGNFSILEAVPA"
     gene            4733..5083
                     /locus_tag="VGR03_15640"
     CDS             4733..5083
                     /locus_tag="VGR03_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995764.1"
                     /translation="MPWDFALLVLLLGVVVPWRSVVRMRQLLRLPATTSEQRLVLYAG
                     TMAAQWLIAGVALWRALSHGIAPRALGLALHHPASTLAIAVPLCGMMAALQIYGLRRL
                     ARLPPRFQAWAFAF"
     gene            5298..5633
                     /locus_tag="VGR03_15645"
     CDS             5298..5633
                     /locus_tag="VGR03_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995765.1"
                     /translation="MPWYFALIVLLLGIVIPWRSLVRMRQLLRLPATTSGQRLILYAG
                     TMAAQWLLAGVALWRALAHGIAPRALGLALDYPASTLAIVVPLCGMMAALQIYGLRRL
                     ARLPPRFQA"
     gene            5872..6699
                     /locus_tag="VGR03_15650"
     CDS             5872..6699
                     /locus_tag="VGR03_15650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEV1995766.1"
                     /translation="MPWDFALLVLLLGVVVPWRSAVRMRQLLRLPATTSQQRLILYAG
                     TMAAQWLLAGFALWRALAHGIAPRALGLALDYPASTLAITVPLCGMMAALQIYGLRRL
                     ARLPAGQQGYAGVLARKLMPQNPLEALAFVALAATVAVCEEFLYRGFLLAAFAGAIGA
                     GAAGAGGDTLGAAAQAASGTLILAGALSTLLFALGHLYQGVRGVVSTFVLGLVFCALR
                     IATGSLVPCVAIHFGVDLVAGLAAPRLLSAASGDQDASAQQDPAPPAGQSTAQQKPV"
     gene            6813..7406
                     /gene="rsmG"
                     /locus_tag="VGR03_15655"
     CDS             6813..7406
                     /gene="rsmG"
                     /locus_tag="VGR03_15655"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV1995767.1"
                     /translation="MRPFAVPLTPALADSLRQYLALLLRWNQKINLTATSDVGELLRR
                     HVGESLFGAKLLPAKGGTLYDIGSGAGFPGIPLKLARPDWELVLVESDHRKAAFLSEA
                     VRTLGLGGTRVLAERFDQLAAQNGLADVITVRALGQYEILLDLAIRFLRPSGRVILWL
                     GAEDASRLSGVHGWRWDAKILVPESRERVVLVGTLDA"
     gene            7562..8347
                     /locus_tag="VGR03_15660"
     CDS             7562..8347
                     /locus_tag="VGR03_15660"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1995768.1"
                     /translation="MGKIIAIANQKGGVGKTTTAVNLAASLAAAEQRTLLVDCDPQGN
                     ATGGLGFKKDPARRSLYHGLILEEPTVRIIVKTQMEGLDMLPSDKNLVGAAIELVSME
                     EREYRLRKLLAPLKEHYSYILLDCPPALDLLTLNALTAADSVLIPIQCEYFALEGVTE
                     LLDTLMRIRRTLNPGLAVEGIVLTMYDERTTLSRQVSADLRSFFGAQVFRTVIPRNVR
                     LAEAPSHGKPIIFYDIHSKGAESYIQLAKEVISNGQARFGEGS"
     gene            8316..9188
                     /locus_tag="VGR03_15665"
     CDS             8316..9188
                     /locus_tag="VGR03_15665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV1995769.1"
                     /translation="MARLALGKGLSALIREPEPEVKIAPPAPSSSGSLEVDIDLIDPN
                     PFQPRTSFREQALEELAQSIRSTGVIQPIVLRRTGARYQLIAGERRWRAAQRAGLHRI
                     AAVIRDVPEELALEMTLVENLQREDLNPVEQARAFERLMAEFHLTQEDVAVRTGKDRT
                     TIANAVRLLMLDAPIIEMLEDGRLTAGHGRALLAIHERSLRIMLADQAARKWLSVRQL
                     ERLSSRQARSPKKTVGWSAVDANTRAALEELQRDLGTRVLFRPLTGKHPGRLILEFYD
                     NAQLISLYERLRAR"
     gene            9301..9714
                     /locus_tag="VGR03_15670"
     CDS             9301..9714
                     /locus_tag="VGR03_15670"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEV1995770.1"
                     /translation="MKLFDKKSAQESGDWVGFLDKGVKLDGRLELSGAFRLDANLKGT
                     IVSNSTLVIGENARVEGEIQGNQVVVSGRFEGSLTAKGRVEIQAKGVVRGEIHSPCLM
                     IEAGGIFDGRCHMTGNAEGQEPVEIPIRAAAATQS"
     gene            10048..13317
                     /locus_tag="VGR03_15675"
     CDS             10048..13317
                     /locus_tag="VGR03_15675"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV1995771.1"
                     /translation="MKTRRSISRNSILQFRGMAGFMLLIFLLPGIGRAQVTTGSLLGT
                     VQDSSGAAIRGATVTTKNLQTGLTRSVLSGEDGGYAINLLAVGDYSIRVEFAGFKADE
                     RSPVTLQINSRARVDFVLQVGKVTEKVEVTGSQPVLDTDTAETGQVIENTRVKQLPLN
                     GRQFVQLTLLTPGVVPEVKGTLSSPLALSGLSVNANGARYESNAYLLDGVPIRDEIYD
                     RLTVSPSVDAIEEFKVHTSNYSAEFGGHGGAQINISTKSGTNDFHGVVYEFLRNDVLD
                     ARNFFDPTKPPFRQNQFGASVGGPIKQNKTFFFGNYEGSRIFKGITITSALPTDALRA
                     GNFAGMGPIIDPTTGTPFLNSQIPPGRIVAFAQAYLNKIPHATTQVLGRNFAGFGNRD
                     VDMNQFTTRIDHSFSDRDQISGRFVFSRVNDTEPFPATVDPSGNPLSPPGFGQTTFQH
                     SSNLAVGYTHIFDPQFLNEFRFGYSFLDVGQHSQNSGVNFPGQFGFQGTNPAPLGAGF
                     PSIVIPGFSNLGDPTTQLFVGNNVFTFSDNVVRNFGKHSMRFGANYKKSNVRTEFVFN
                     SAGQYKFLGVFTNNPFADFLLGFPAVATSLTGDPLLHGLGYGFGAYFQDDWRVTQRLT
                     VNLGIRYDINSPFKERDNKFANFSPEIGGFVIAGSPGHINPAANPGRFPGVPFMTAKE
                     LGYPDALTNGDYGNFAPRLGFAYSPIKTLAIRGGMGIFFDSGLVGGRFGILGFNPPFT
                     GLQLFLNFNPAAPIPAQTALVTPAANIVLGQGPQRDFPNAYLAEWNLSVEKQLGSSFV
                     VEAAFVGSRGKKLDGTLFPNQPSASPAPLAGRVRWPVLGVDLEIASPVFDSWYHSLTL
                     RGERRYSNGFVFSGSYTYAKSLDTGGGSLSNFSDQSNGTPQFSGNVAAEKGRSSFDAR
                     HRFVFNGVYDLPFGHGKTYLNNYQGAAEKFVGGWQLNSIIVLQSGRPETPLIPFDQSN
                     TGANTDRPNVVSNPNTGPRTPNQWFNTSAFQLQPFGTFGNSGRGIINGPRYESVDLGI
                     AKITPVTERIGVEFRAEFFNLFNHANFDLPDRQFSTPGFGQIFSANDPREIQFALKLR
                     F"
     gene            13369..14814
                     /locus_tag="VGR03_15680"
     CDS             13369..14814
                     /locus_tag="VGR03_15680"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEV1995772.1"
                     /translation="MTRSVRLKTCFLMALLFSPMTQAGQAGKRPEKLSPAFHKNRLEE
                     LYRRCPDGLILLRGEVSWYRKREMRAFDAAYADYNFKQERNLYYLTGIEVPDSFVLID
                     PKKKEVRLYTEWKGARELEEVRKLDYVSGPYPPEAFLHDVLVRSPDYAALYALYVPFL
                     EAGNLYGKTGPLTGVFPPGLGEPLSEETQFARRLAETFPNLRVKSLAPALLEMQRIKQ
                     EEEIGLLRRANEIAAHGVVEAIKAIRPGLYDHDINAVIEYVFTRGGGVNPTFALNVMS
                     GPNAFLDLLPLWSDYDHLDRQMKAGEAVFLDVGAEVAYYVSDIGRTAPVSGKFTAEQK
                     KLYDVYLPCFLKAERSIRPGVTQRDLMKICASCAREQLSHLEEGFLRKAVEEFIREVE
                     SHATLGHYQDLNVMGAGAGNDEPLQPGMVFAIEPVLYSKELNFAVFVEDVILVTADGY
                     DVLSKGMPYTTDEIEKLMAQKSIIEASEERH"
BASE COUNT         2889 a         4638 c         4430 g         3001 t
ORIGIN      
        1 atcccccgcg ccgagatgct cttcatcgtc gagcgcatcg cccgcgcgtt ggcggtcccc
       61 gtcaccgccg acatggaagc gggctactcc accacgcccg aaggcgccgc cgaaaccgcg
      121 caagccgtca tcgccgccgg cgccgtgggc atgaacttcg aagactccac cggcaacgcg
      181 caagccccgc tgttcgacct ctcgcttcaa gtagaacgaa ttaaagcaat tcgcttagct
      241 tgcgcttctt ctggagttcc cctggtcctg aacgcccgca ccgacgtctt tctcgagcag
      301 gtgggcgacc ccccctcgcg tttcgaccac gccgtccgcc gcgccaacgc ctaccgcgaa
      361 gccggcgccg attccctctt cgtccccggc gtcagcgacc gcgacgtcat cgcccgcctc
      421 gtccgcgaaa tccatggccc cgtcaacatc ctcgccgttg ccggatcgcc gtccgtcccc
      481 gaactcctac gcctcggcgt ctcccgcgtc agcgtcggct ccggacccca tcgcgccacc
      541 atggccctca cccgccgcat cgccacagaa ctcctcgcca ccggcaggta ctcatccttc
      601 acccaggaca ccatcaccta cgccgaggcc aacgaactgt tcaagcgcgc gtaacctgta
      661 ggggcggctt cacaccgccc tgtctcggac tatgtagcgg cggcctctag gccggcatct
      721 ttctccaccc gcatgttcct tgtgcgaagt cgctcgttgt ccgaccatct ccagccaaat
      781 acagatgcgg attacacgat tatcgactta acccaattag agaatggcgc tactattttc
      841 ccatgaacat ccgagcgatc accgccaaat ttgccgaagc tggcaaacca ttccttgcgc
      901 tctggggagc ggtccgatgg atagggcaga gattttggac atgggagctt gtctcttggt
      961 tggtgggagt tgtgtttgta gggctcgcct ttgtgctcgc ccaatacgga tggtatctga
     1021 gcgggcgtct gttcttagtg ctaggagcaa ttctcctcat cctgcggatc gtgcatgatg
     1081 caatgaagtc caaggacatc agtgtcgcag agaagatagt tgcaagcata atcgtcaccg
     1141 ccgtcctatc cggcatctgc gctggtggtt cggcattcat caatgacatc gaatggaaaa
     1201 atgaaataag aatacatctc tcgtttaaat cctctccctt atttacaaag cagcgcgaaa
     1261 agaaaattat ttgggagctc aacagctact atcgctacct caccgagttg gggctcgact
     1321 tgtcgccaga aattccccca ttgggaatca caccaccgca tggcccgatg tcagcgggtg
     1381 gacaaccgaa tgcaccgttc tactattcgt cgctatatat ttccgaggat gtcatcgaca
     1441 accccgacat ggttaaggct atgtattcaa cctacatttt cggtcgcctt gttagagaca
     1501 gcacaaatac aacttagcga cagcaatcgc taaatgaagg aaccgcggtg tggatttttc
     1561 agtgctactt cccgtgacct gccccctttt ttcgtccact tcatgatacg acttccgtgg
     1621 cctgctcccc aatcttggtc cagtcatatt gtgaccgccg acggttcttc ccgtttttct
     1681 tccccgatgc tctggagttg agcctcggtc gtgaccggca cgaaccgctc cgcgcccgcg
     1741 taggctgccg cgtactcttc cggcgtccgg tatcccagac tgctgtgcgg ccgcacgtgg
     1801 ttgtactcct gccgccacgc ggaaatcttc ccccgcgcgt cccacaggtt ctcgaaccag
     1861 ctcgcgttca gacactcctc gcgcaaccgc ccgtgaaagc tttccacctt cgcattttgc
     1921 accggcttgc cgggttcgat gtagtccagc gcgatcctct tctccactcc ccaagccaga
     1981 aaatgccggc tggtgaattc cggtccgttg tccagccgca gacttgccgg cgccgcgcgt
     2041 tccgccatca cctggcccaa cacccgcgtc acccgcggac cgcaaaacga cgtgtccaca
     2101 tccagcgcca ggcattcgcg cgtgtacgca tcgatcacgc tgaacacgcg aaacgcccgc
     2161 ccgtaggcca gtcggtcctg cgcgaaatcg atggcccact gctgattcgg cccggtcagc
     2221 gtctccgccg cgcgcagcgc ccgcaccagc cgcttcctcc gtttccgcct catgcacaac
     2281 ccggcctcgc ggtacacccg cagcagccgc ttgtggttca cggccatccc ctcgcgctcc
     2341 accaccagca cctgcaaccg ccggtatccc cagcgcggat gctccgccgc caactccttc
     2401 agccgcacgc gcagcacttc gttgcgatcc ggccgccgct gataccggca actcgaccgt
     2461 gctatcacca gcagctcgca ggcccggcgc tcgctcaccg cgtagtgctc gcgcaccagc
     2521 gccacctcct gacggcctac gagctccatc cgttttttga gatcaccgcc ttcagcatct
     2581 ctttatccag gctgagatcc gccaccaact gcttcaaccg ccggttctcc tcctccagtt
     2641 gccgcagccg ttgcgcctcg ctcacgctca tcccgccgta cttcgccttc caggcgtaca
     2701 cggtgtgctt ggacacaccc atctcccgag cgacttcgat cagggtgcgc cctgcctcac
     2761 cctgcttcag agccgcgatg atctccgctt ccgtgaactt ccgttttggc attgccctgc
     2821 ctcctcaaat ggcccaaaat catatctgac tttggaccag tttttgggga gcaggtcacc
     2881 cgcaaagctt cagcggccgc ccagtgtgcg actcgcaaag ccctggctat aagtggaccg
     2941 ctgcgctctt ggacattaga cagaaatacg aaccacgata caccgatacg ctcctctgct
     3001 atacgctgaa aatgtggaag aaagttcctt catcgaaata tctggataac ttcgaccagt
     3061 atttcaggta caagctaacc gcaggagaat cgtttgccga caatatgggt ggcgttagat
     3121 atcgtgaatt gaatgatatt cttaagaaac acgatatcga tgtttctcag ccttagaact
     3181 tgcggatgac tacttctgcg acgggtgcct cggttgattt cgagtagtga attcggggac
     3241 ctgtctcaaa cctacgatca gttcggccag cggcttgttc cttcttctcc tctccactcg
     3301 cgcgccctga ggtcggcaga gccgaccgaa gggtcacccg cctgcccgcc gaagccttgg
     3361 cggaggaggg tcactgtcta cccacgctct cccccgcgac cgcagtgaca ttccggagca
     3421 tttctcccga ccgtctaggc ggatggccga ggcgaacgag cttttcaaga gtgccaggta
     3481 ggattgctaa cgccgggaat tctccggtca cgtcaagccg gcaccgcttc gagaattgaa
     3541 aagttgccgc cgacatcaat atcccgctcg agccgaaagc ccgccccagc gagcaacttg
     3601 gcatgttcac tccgcgtgcg ctgccggccg gtaagcagcg tcatcatgtg aatgtccaac
     3661 accttggcaa aattcgggtg aggatcatcg gggataatgg cttcgatgag cagcagcttg
     3721 gcgtgggcgg gagccgcgcg gcggatattc ttgaggatcc tgatcgattc atcgtcgccc
     3781 cagtcgtgaa tcacgtccat gagcaggtag gcgtcacacg ccggcatggt gtcgttgaag
     3841 aaatcccccg cctggagccg gagccggtcc gaggcttcgc tggaagctcg ttcgatcacc
     3901 tgcggcaaat caaataacac gcccttggca gtcggcgtcg cggccagcac cgcctgcaac
     3961 aggtggcccc ggccgccgcc gatgtcgcca atcaggccga atccggaaaa agggtagccg
     4021 ccgacgaccc cgccaatctg cccttgcgcc ttgcccgtca tggcctgatc gaaaatccgt
     4081 cccgcctccg ggtgctcaac aaagtagctc caaactccgc cagggaccac tttctccatg
     4141 gccgggcggc ccgcccgtag ggtgtgctcc agcgactggt aggacgccca aaagctcgga
     4201 agaccaatca ttcgtacaaa ggcgcgcatc gactgcggat ggtcggtccg caacagccga
     4261 gaagcggggg tgtggacata tccgtcgtcg cgaccttcaa atatcccgtg cgctgaaagc
     4321 aggcgcagca cgcgccccag cgccccggca tcggttccgg tcgcttctgc cagagccgct
     4381 gcggtccgcg gcgaatcgtc cagcgcatcg gcaaccccga ggtcggcaac cacgtgcagg
     4441 catcgcgata aggcataggc gctggagatc tgcatcagcg tgtcgaaagg tgtggtttcg
     4501 gccatgatgt ccctccgcgg aaggaactta taacagactt ccggcagcca cgaaacggtg
     4561 cgtcgcacgc ctcccgtccc agagctccaa cagctcggcg tcgcccggtg ccgcattcct
     4621 gcgtagcaag tgtaagaaac ctgctccctt ccgctcatct gctccattca accctgaccc
     4681 ctgtccgttt cccctttccc tctgacccct ataccctatc ccctgaacta ccatgccctg
     4741 ggatttcgcg ctacttgtgc tgctcctcgg agtcgtcgtt ccatggcgct ctgtcgttcg
     4801 aatgcgccag ttgctgcgtc tcccggccac caccagcgag cagcggctgg ttctctatgc
     4861 cgggaccatg gccgcccagt ggctcatcgc cggggtcgca ttatggcgcg ccctgtctca
     4921 cggaatcgct ccccgcgccc tgggcctggc gcttcaccac cccgcgtcca ccctcgcgat
     4981 cgcagtcccg ctctgcggga tgatggccgc gctccagatc tacggcctgc gtcgcctcgc
     5041 ccgcctcccg cccaggtttc aagcgtgggc ttttgcgttc tgaaatttgt tcgtccaaga
     5101 acccttcctt gctgccgcag gcggtcgcgt aggccccgtc cgaagcgaca atcctcacag
     5161 cagaatgttc gcgtcgagcg ctaggccctt gcccgcacac gtttgccacc gccggcgggc
     5221 caacctcccc gccacatcgc atccccttcg cccctggccc cttgtacctg ctccccgtcc
     5281 cctgtaacct gcataccgtg ccctggtatt tcgcgcttat cgtgctgctc ctcggaatcg
     5341 tcattccctg gcgctccctc gttcggatgc gccagttgct gcgcctcccc gcaaccacca
     5401 gcgggcagcg gctcattctc tacgccggga ccatggcagc ccagtggctc ctcgctggcg
     5461 tcgcattatg gcgcgccctg gctcatggaa tcgctccccg tgcgctgggt ctggcccttg
     5521 actaccccgc gtccaccctc gcgatcgtag tcccgctctg cgggatgatg gccgcgctcc
     5581 agatctacgg cctgcgccgt ctcgcccgcc tcccgcccag gtttcaagcg tgagcttttg
     5641 cgttctgaaa tttgttcgtc caagaaccct tccttgctgc cgcaggcggt cgcgtaggcc
     5701 ccgtccgaag cgacaatcct tgcctggctc gcagcagaat gttcgcgttg cgcacgaggc
     5761 ccttgcccac actcatgcgc ggccgccagc gcgtccaccg cccggccgca tcgcatcccc
     5821 tcgccgctgc ccccttgtac ctgctccccg tccccggtaa gctgccttcc catgccttgg
     5881 gatttcgccc ttctcgtgtt gctcctcgga gtcgtcgttc cctggcgctc ggccgtgcga
     5941 atgcgccaac tgctgcgcct cccggccacc accagccagc agcgcctgat tctctacgcc
     6001 gggaccatgg ccgcccagtg gctcctcgct ggcttcgcat tatggcgcgc cctggctcat
     6061 ggaatcgctc cgcgcgctct aggtctggcc cttgactacc ccgcgtccac cctcgcgatc
     6121 acagtcccgc tctgcgggat gatggccgcg ctccagatct acggcctgcg ccgcctggcc
     6181 cgcctcccgg ccggccaaca aggctatgcc ggcgtcctgg cgcgcaagct gatgccgcag
     6241 aatcctcttg aagcgctggc gtttgtggcg ctggccgcca ccgtggctgt ttgcgaggag
     6301 ttcctgtatc gcggattcct gcttgcggcg ttcgccggtg ccataggcgc tggcgccgca
     6361 ggcgcaggag gcgatacgct tggtgctgcg gctcaggctg ccagcgggac gctgatcctt
     6421 gcgggtgcgc tgtcgaccct gctcttcgct ctcggtcatc tctatcaggg agtccgcggt
     6481 gtcgttagca cattcgttct ggggctggtt ttctgcgctc tgcgaatcgc cacggggagc
     6541 ctggtcccgt gcgtggccat ccactttggc gtagatctgg tggccgggct cgcggcgccg
     6601 cgcctgctct ctgctgccag cggggatcaa gatgccagcg cgcagcaaga ccccgcgccc
     6661 ccggcgggcc aaagtaccgc acaacagaag ccagtatgaa aaggaaaggc cagccacccc
     6721 aagaccgcgc ccgcgggctt gaatccaggg gtaaatctac attaactaat agtaaagaac
     6781 aacatataga gtatagcttt atcgaggaag ccttgcggcc cttcgccgtc cccctcactc
     6841 ccgccctggc cgactccctc cgccaatacc tggccctcct cctgcgctgg aatcagaaga
     6901 tcaacctcac cgcgacctcc gacgtcggcg agctcctccg ccggcacgtc ggcgagagcc
     6961 tctttggcgc gaagttgctc ccagcaaagg gcggcacgct ctacgatatc ggctccggcg
     7021 cgggtttccc ggggatcccg ctaaaactcg cgcggccgga ttgggagctg gtgcttgtgg
     7081 agtctgatca ccgcaaggct gccttcttat ccgaggccgt gcgaactctc ggtctcggag
     7141 gaactcgagt tctcgccgag cgtttcgatc aactggccgc acagaacggc ctggcagatg
     7201 tcatcacggt tcgcgccctg gggcagtatg agatccttct cgatttggcg atccggttcc
     7261 tgcgaccgtc cgggagagtg attctttggc tgggtgcgga ggatgcgagc aggctttcag
     7321 gagtccacgg ctggaggtgg gatgccaaaa ttctcgttcc tgagtcaaga gagagagtcg
     7381 tgctggttgg gactctggat gcctagaaaa tactcctgat atctttgatc gaccgttcca
     7441 cgtggaacag ttcgtttgag ccgatcattc cggttaggta acataattct tggttccacg
     7501 tgaaacatct tgtctcggcg agttctaatc tgctagattg cattggatcg cgggggcctt
     7561 cttggggaag atcatcgcca tcgccaacca gaagggtggc gttggaaaaa cgaccacggc
     7621 agtgaatctt gccgcttctc tcgctgcggc ggagcagcgc accctcctcg tcgattgcga
     7681 tcctcagggc aacgctacgg gcggtctggg gttcaaaaaa gacccggcac ggcgctccct
     7741 ctatcatggg ttaatcctcg aagagccaac cgtcagaatt attgtgaaga ctcagatgga
     7801 agggctcgat atgctgccat ccgataagaa cctcgtgggc gcagcgatcg aacttgtctc
     7861 gatggaggag cgagaatatc ggctacgcaa gctcttggct ccgttgaagg agcactacag
     7921 ctatatactg cttgactgcc ctccggcgct ggacctgctg acgctgaacg cactcactgc
     7981 ggcggactcc gtactgatcc cgattcaatg tgaatacttt gcgctggaag gcgtgacgga
     8041 gctgctggac acgttgatgc gaatccgcag aacgctgaac cccggcctgg ctgtggaggg
     8101 aatcgtactc accatgtacg acgagcggac cacgctgtcg cggcaagtga gcgcggacct
     8161 gcgcagcttt ttcggagcgc aggtatttcg cacagttata ccacgaaatg ttcgattagc
     8221 tgaagcgcca agtcatggta agccgattat tttctatgac atacacagca aaggggcgga
     8281 gagttacatc cagctcgcca aagaggtgat ctcaaatggc caggctcgct ttggggaagg
     8341 gtcttagcgc tctgattcga gagccggaac cggaggtcaa gattgcgcct cccgcgccgt
     8401 cgagttcggg gtcgctggag gtggacatcg acttgatcga tccgaaccct ttccagccgc
     8461 ggactagttt ccgcgaacag gcgcttgagg agctggcgca gtcgatccgg tcgacgggcg
     8521 tgattcaacc gatcgtgttg cggcgcacgg gcgcgcgata tcagctcatt gccggggagc
     8581 ggcgctggcg cgcggcgcag cgcgcaggat tgcaccgcat tgccgcagtg atccgcgatg
     8641 tgccggagga gctcgcgctg gagatgacgc tggtggaaaa tctgcagcgc gaggatttga
     8701 atccggtgga gcaggcgcga gctttcgagc gcctgatggc cgagttccat ctaacccagg
     8761 aagacgtcgc ggtgcgcacg ggaaaagatc gcaccacgat cgcgaatgcg gtccgtctgt
     8821 tgatgctgga cgcgccaatc atcgagatgc tggaagacgg ccggctgacg gccggtcatg
     8881 gcagggcgtt gctggcgatc cacgagcgct ctttgagaat tatgctggcg gaccaagcgg
     8941 cgaggaagtg gctctccgtc cggcagcttg agagattgag ttcccggcaa gcgcgcagcc
     9001 ccaagaagac ggtgggctgg agcgcggtgg acgcgaacac gcgcgccgcg cttgaagagc
     9061 tgcaacgcga cctggggacg cgtgtgcttt ttagaccgct aacaggcaag catcctgggc
     9121 ggctgatcct ggaattctat gataatgcac agcttatctc gctgtacgag cggctgcggg
     9181 cacgttagcg cgcgggaagg gaacgggcgc gggcttttaa cttgacaggg gtcatgcgtc
     9241 ccgatatgac acctgtctca gcccttttca acaccctact tgctgggaag gggcaatgcg
     9301 atgaagctct tcgacaaaaa aagcgcacag gagtccggcg actgggtcgg cttcctcgat
     9361 aagggggtca agctggacgg gcggctcgag ctttcgggcg cgtttcggct ggacgccaat
     9421 ctgaagggaa cgatcgtctc aaactcgacg ctggtgattg gggaaaacgc gcgcgtggaa
     9481 ggggagatcc aggggaatca ggtggtggtc tccgggcggt tcgagggatc cctgacggcg
     9541 aagggccgcg tggagattca ggccaagggc gtggtccgcg gagagattca ttcgccgtgc
     9601 ctgatgattg aagccggcgg gattttcgac gggcgctgcc acatgaccgg caacgcggaa
     9661 gggcaggagc cggtggagat accgattcgc gccgccgcgg cgacgcagag ctagcagccg
     9721 cgagagccag acccaccttc ggactaccca ttcaccaatc tgctcgcgaa gtgaaaatca
     9781 accgttctgg ctgcggccat gtgtcgcggc gatacgaggc cagccactgg gatctctgtg
     9841 ctccgtagtg atacgtaccg taaggctaat cgcttttcaa tttaagagga gaaggcggcc
     9901 gcggaggagt agaatccccg ccaggcatgc gggcggaggc cgctcgtcca aacgggaaag
     9961 tccgcttcat gccgatagtg cggatcggag aagggcggag cggattaggg tgcacctggc
    10021 ggacgaaaga acttcgtgga gggaatcatg aagactcggc gctcgatttc acgaaattcc
    10081 attctgcagt tccgcggaat ggcaggcttt atgcttctga ttttcctgct gcccggcatt
    10141 ggtcgcgccc aggtgacgac gggaagcctg ctggggaccg tacaagattc ctcgggcgcg
    10201 gcgattcgcg gcgccacggt gacgacgaaa aacctgcaaa ctgggctgac gaggtcggtg
    10261 ctttccgggg aggatggagg atacgcgatc aacctgctgg cggtggggga ttactcgatc
    10321 cgcgtagagt tcgccggatt caaggcggac gagcggtctc cggtgacact gcaaatcaac
    10381 agccgcgccc gcgtggattt tgtcctgcaa gtcgggaagg tgacggaaaa ggtggaagtc
    10441 acggggagcc agccggttct ggacacggac acggcggaaa cgggacaggt gatcgaaaac
    10501 acgcgagtga aacagcttcc gctgaacggg cggcaatttg tgcagttgac actgttgacg
    10561 ccgggagttg tgccggaggt gaagggcacg ctaagcagcc cgctggcgct atcggggctg
    10621 tcggtgaacg cgaatggtgc gcggtatgag agcaacgcct atctgctcga cggggtgccg
    10681 atccgcgacg agatttacga ccggctgacg gtgtcgccgt cggtggatgc gatcgaggaa
    10741 ttcaaagtcc acaccagtaa ttattcggcc gagttcggcg ggcacggcgg ggcgcagatc
    10801 aacatctcga cgaagtcggg tacgaatgat ttccatgggg tggtgtacga gttcctgcgc
    10861 aacgacgtgc tggacgcgag aaactttttt gaccccacga agccgccctt ccggcaaaac
    10921 caattcgggg ccagtgtggg cgggccgata aaacagaata agacgttttt cttcggcaat
    10981 tacgagggca gccgcatctt taaaggaatc acaatcacct cggcgctccc cacagacgcc
    11041 ttgcgggcgg gaaacttcgc cggcatgggc ccgatcattg atccgacaac ggggaccccc
    11101 ttccttaatt cccagattcc gccggggcgg atcgtggcct tcgcgcaggc gtacctgaac
    11161 aagattcctc atgccacgac acaggttttg ggcaggaatt ttgcgggttt cgggaaccgt
    11221 gacgtggaca tgaaccagtt cacgacgcgg atcgatcatt ccttttcgga ccgcgaccag
    11281 atctctggcc gtttcgtgtt ctcccgcgtg aacgataccg agccgtttcc ggcgaccgtg
    11341 gacccgtcgg gaaatccgtt gagtccgccg ggattcggac agaccacgtt tcagcacagc
    11401 agcaatctgg cggtgggata tacgcacatc ttcgatccac aattcctgaa cgagttccgg
    11461 tttggctaca gtttcctcga cgtgggacag cattcgcaga attccggcgt caatttcccg
    11521 ggccagttcg ggtttcaggg cacgaacccg gcgccgctcg gggcgggttt cccgagcatc
    11581 gtgattcccg gattcagcaa cctgggcgac cccacgacgc aactgttcgt ggggaacaac
    11641 gtgtttacgt tcagcgacaa cgtggtgcgc aatttcggca agcattccat gcgcttcggg
    11701 gcaaactaca agaagagcaa tgtgcggacg gagttcgtgt tcaacagcgc gggacaatac
    11761 aagtttttgg gcgtgttcac gaacaacccg tttgcggatt tcctgctggg attcccggcg
    11821 gtggccacgt cgctgacggg agatccgctg ctgcacgggc tgggatacgg gttcggcgcg
    11881 tacttccagg acgactggag agtgacgcag cgcctcaccg tgaatctggg aatccgctac
    11941 gacatcaatt cgccgttcaa ggagcgggac aataagttcg cgaatttctc gccggagatc
    12001 ggcggcttcg tgatcgccgg aagtccgggc cacattaatc ctgcggcgaa tcctgggcgg
    12061 tttccgggcg tgccgttcat gacggcgaag gagttgggat atccggatgc gctcaccaac
    12121 ggggactacg gcaacttcgc tccgcggctg ggctttgcct attcgccgat caagacgctg
    12181 gcgattcgcg ggggcatggg aattttcttc gacagtgggc tggtgggagg gcggttcggg
    12241 attttgggat tcaacccgcc gttcaccggc ttgcagctct tcctcaactt caatccggca
    12301 gcacccatcc cggcgcaaac tgcgctggta acgccggcgg cgaatatcgt cctgggacag
    12361 ggcccgcaga gggattttcc gaatgcgtac cttgcggaat ggaatctgag cgttgaaaag
    12421 caactcggat ccagctttgt ggtggaggcg gcgtttgtgg gatcgagggg aaagaagctg
    12481 gacggtacac tgtttccgaa tcagccgagc gccagcccgg cgccgctggc ggggagggtg
    12541 cggtggcccg tgctcggggt ggacctggaa atcgcgtcgc cggtattcga ctcgtggtac
    12601 cacagcttga cgctgcgcgg cgagaggagg tatagcaacg gatttgtatt ttcggggagc
    12661 tacacgtatg cgaagtcgct ggacaccggg ggagggtcgc tctccaattt ttcggatcag
    12721 agcaacggga cgccacagtt ttccgggaac gtcgcggcgg agaaagggag atcgagcttc
    12781 gacgcgcggc accgcttcgt gttcaacggg gtgtacgacc ttccgtttgg tcacggaaaa
    12841 acatatttga acaactatca aggggccgca gagaaattcg tgggcggatg gcagttgaat
    12901 tcgatcatcg tgttgcagtc ggggagaccg gaaacgccgc tgattccgtt cgaccagagc
    12961 aacaccggcg ccaacacgga ccggcccaat gtggtctcga atcccaacac gggtccgcgg
    13021 actccgaatc aatggttcaa caccagcgcc ttccaactgc agccgttcgg caccttcggg
    13081 aattccggcc ggggcatcat caatggcccg cggtatgaga gcgtggacct ggggatcgcc
    13141 aagatcactc ccgtcaccga acgcatcggc gtggaattcc gcgcggagtt tttcaacctg
    13201 ttcaaccacg cgaacttcga tctgcccgac cgccagttca gcacgccggg gttcgggcag
    13261 atcttcagcg ccaacgatcc gcgggagatt cagttcgctc tcaaattgcg tttctaagcg
    13321 gattccggca gagatgattt tccgccgcta aggggagggt gtgccgtgat gaccaggagc
    13381 gtgcgactca aaacctgctt tctgatggcg ctgctgttca gcccgatgac acaagcagga
    13441 caggcgggaa agcgaccgga aaaattgtct cccgcgtttc acaagaatcg cctggaagaa
    13501 ctctaccggc gctgtccgga tggactgatc ctgctgcggg gagaagtaag ctggtaccgc
    13561 aagcgcgaga tgagagcctt cgatgctgcc tatgcagact acaactttaa acaggaaagg
    13621 aatctttatt acctaacggg gatcgaggtg ccggactcct ttgttctcat tgacccaaag
    13681 aaaaaagaag tgcggttgta cacggaatgg aagggcgcga gagagctgga agaagtccgc
    13741 aaactcgact acgtgagcgg gccgtatccc cccgaggcgt ttctgcatga cgtgctggtg
    13801 cgctcgccgg actacgcggc gctgtacgcg ctgtatgttc cgttcctcga agccggaaat
    13861 ctctacggaa agacgggacc gctgacgggc gtctttccgc ccggacttgg cgagcctctc
    13921 tcggaagaga cgcagttcgc gcggcgcctt gcggagacgt ttccaaacct tcgggtaaag
    13981 agcctggctc cggcgcttct ggagatgcag agaatcaagc aggaggaaga gattggcctg
    14041 cttcgccggg cgaatgaaat cgccgcgcat ggggtggtcg aggcgatcaa ggccatccgg
    14101 cccggcctgt atgaccacga catcaacgcg gtgattgagt acgtgttcac gcgggggggc
    14161 ggcgtgaatc caacgtttgc tctcaatgtg atgtccgggc ccaacgcgtt tctggatttg
    14221 ctgccgctgt ggagcgacta cgaccacctc gaccggcaga tgaaggcggg agaggcggtg
    14281 ttcctcgacg ttggggcaga ggtggcctat tacgtgagcg acatcggaag gactgcaccg
    14341 gtgtccggaa agttcaccgc cgaacagaaa aaactgtatg acgtctatct cccctgcttc
    14401 ctgaaggcgg agcgcagcat acgtccgggg gttacccagc gcgacctgat gaagatttgt
    14461 gcgagctgcg ctcgcgagca gctctcccat cttgaggaag gtttcctgcg caaggcggtg
    14521 gaggagttca tccgggaggt ggagagccac gcgacgctcg gccattatca ggacctgaac
    14581 gtgatgggcg cgggagcagg caatgacgag ccactccagc cggggatggt gtttgcgatc
    14641 gagccggtgc tctattcgaa ggaattgaac ttcgccgtgt tcgtggaaga tgtgattctg
    14701 gtgacggcag atggatacga tgttctctcc aaggggatgc cgtatacaac ggacgaaatc
    14761 gaaaaattga tggcgcaaaa aagcatcatc gaagcaagcg aagagagaca ttagctcgac
    14821 atcgccaagc gagagactcc tcccttccat ccagcaaccg accgcaacgc tttcagaaat
    14881 atcgaacagg catcgcgaag gagggtgcgg acccacgcgg gctcacccgc gcacccgcca
    14941 aaacgcactc actcgcgc
//
LOCUS       DASYVO010000459         7058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_235102, whole genome shotgun sequence.
ACCESSION   DASYVO010000459 DASYVO010000000
VERSION     DASYVO010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7058
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235102"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VGR03_15685"
     CDS             complement(<1..205)
                     /locus_tag="VGR03_15685"
                     /inference="COORDINATES: protein motif:HMM:NF012510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEV1995773.1"
                     /translation="MFRKILIANRGEIAVRIVRACRELGIRSVAVFSDVDRKSLHVRL
                     ADQAYPIGPAPSRESYLCINKLMD"
     gene            532..2265
                     /locus_tag="VGR03_15690"
     CDS             532..2265
                     /locus_tag="VGR03_15690"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEV1995774.1"
                     /translation="MLIATRFAETPQLHLLKTPGGARQQLTFFADSVGGGRFHPNGGD
                     YILFAKDIGGGEWYQLYRYDMATGDVTLLTDGKSRNLPGPWSSSGDQIAYMSTRRTGK
                     DTDLWVMNPADPKSDHLLKELEGGGWQPLDWSPDDKKILLEEELSINESYLWLVDTTT
                     GEKTELTPRNTTERVSYGEGRFSKDGKGIYVTTDKASEIHRLAYVDLESKQHWYLTSE
                     IPWDVDMFDVSQDGKRIAFVTNEDGLSVLRWFSTDHKPVVVQKSPNGVIGGVRWHKNG
                     RELGFSLANAQGPGDVYSVDVTTLKIEQWTTSETAVKTGAFPQAALVEWKSFDGKTIS
                     GFLYKPPAKFTGKRPVLVVIHGGPEGQSQPTFLGRANYLLNELGIALIYPNVRGSTGY
                     GKTFSLLDNGFKREDTYKDINALFDWIATQPGLDSERIAVTGGSYGGHMTLAVSTFYS
                     DRIRCSVDIVGMSNLVTFLEHTEAYRRDLRRVEYGDERDPKMREFLEKIAPMNNIEKI
                     KKPMFVIAGKNDPRVPVSESQQIADALKKQGTPVWLLIAKDEGHGYRKKPNQDFQFYA
                     TVEFLQEYLLK"
     gene            complement(2304..3005)
                     /locus_tag="VGR03_15695"
     CDS             complement(2304..3005)
                     /locus_tag="VGR03_15695"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEV1995775.1"
                     /translation="MLASADGCKGGWLLVKSKSWPCKEPLFLSICADFRSLVQSTLDC
                     EQVVVDIPIGLPSGKKIRTCDEDARRLLGREGSTRVFLAPPREAMHASDPQEFQILHS
                     KFRGKGAGWPVWGIVRKLKEVDEIMTPDLQKRIHEFHPELAWWRAAGRVLDSKHQKSG
                     LSQRTEIVGSYISGLNEALEWERRLGRAAAIDDILDALIGIPVAANALNEKTFRVPHQ
                     STESDTRGLSMEIWY"
     gene            complement(3012..4601)
                     /locus_tag="VGR03_15700"
     CDS             complement(3012..4601)
                     /locus_tag="VGR03_15700"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEV1995776.1"
                     /translation="MAETKPSLVPEDKHRDRMEELRRISDEAEAGGGPERREREHKAG
                     KLTARERIHLLLDEGTFEELDKFVRHRCVDFGMQEQRPAGDGFVTGFGRIDGRLVYVF
                     AQDFTVFGGSLSETNALKICKIMDLAMRAGAPVIGLNDSGGARIQEGVASLAGYADIF
                     LRNTLASGVIPQISAIMGPCAGGAVYSPAITDFIFMTRETSYMFVTGPDVIKTVTHEE
                     VTKQELGGAMTHNATSGVAHFVSRDDADCLAMIRELMSFLPSNNLEDPPRRATSDSPA
                     RRDESLNTLVPADPQKPYDIKDVLHSVVDDGYFFEVHEHYAKNLVVGFARLDGRPVGI
                     VANQPAFLAGVLDINASVKGARFVRFCDAFNIPLITFEDVPGFLPGTQQEFGGIIRHG
                     AKLLFAFAEATVPKITVITRKAYGGAYCVMASKHIRTDANFAWPTAEIAVMGPEGAVN
                     IVHKRELDKAPESEREKLRQEKIAEFRERFANPFVAAERGYIDAVIEPADTRARIITS
                     LRALENKRDTNPRKKHGNIPL"
     gene            complement(4594..4842)
                     /locus_tag="VGR03_15705"
     CDS             complement(4594..4842)
                     /locus_tag="VGR03_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995777.1"
                     /translation="MIIASFLTTILIVAMTLGAMFILVRATEYVTALESPLQRAVAMG
                     AELLLGVVLLLGTVWLATHLAVRIFTPKESPSGGGPVG"
     gene            complement(4967..5419)
                     /locus_tag="VGR03_15710"
     CDS             complement(4967..5419)
                     /locus_tag="VGR03_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995778.1"
                     /translation="MKDLLADWKVVLAMGGAAIAALAMIAYAFLRPAVNPEEEERKRR
                     LHLNQIGRIAEGQIVELVEHPPESKEARKGLFGAGARPLSDMRPRHLVSYSYLISGVT
                     YQTAQDITGLESQIRLERVVAGQPASIKYDAANPSDSILVADDWSGLR"
     gene            complement(5563..6351)
                     /locus_tag="VGR03_15715"
     CDS             complement(5563..6351)
                     /locus_tag="VGR03_15715"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV1995779.1"
                     /translation="MKICRYQMRDATSSDSSSPRYGLIEGENVIEISGPPWGPWSRGS
                     RTSRLADVRLLAPVAPSKIVCIGRNYAAHAAEMGNEVPKEPLMFLKPPSSIIGPEEAI
                     VLTKYSQRVEHEGELALVIGRRCSQLSDAEDALSFVVGYTCLNDVTARDLQKSDVQFT
                     RGKGFDTFCAVGPHIETALDPSSVLVETHVNGARRQSGATSLMTYSPAFLVRWISRMM
                     TLMPGDVIATGTPAGVGPLVAGDTVEVSVAGVGVLRNPVHAPQA"
     gene            complement(6358..6816)
                     /locus_tag="VGR03_15720"
     CDS             complement(6358..6816)
                     /locus_tag="VGR03_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995780.1"
                     /translation="MLVRPHLPGYRWFHVFRNAAIRTGVYVGVCLTLVFTAWLVIANH
                     APFLERFAMERNIAAAAIFGFLAAVPIFRFLRLPGHLLASSLLGWLIFSLSYGALCLI
                     FHGLSNRLSTFQVFMLGAVVYLILTTLSWIVATIWRARGTHASHPNHHAS"
     gene            complement(6861..>7058)
                     /locus_tag="VGR03_15725"
     CDS             complement(6861..>7058)
                     /locus_tag="VGR03_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995781.1"
                     /translation="RVYRSEQEGTRGQLLGADLLPTPAVRDTSVEPGHRYWYTVTAVD
                     RAGNESAPGAPVAVDVTSPSP"
BASE COUNT         1596 a         1878 c         2237 g         1347 t
ORIGIN      
        1 cgtccatgag cttattaatg cagagatagc tttcgcgcga gggcgccggg ccgatgggat
       61 acgcttgatc ggcgagtcgg acgtgcagcg atttgcggtc gacatcactg aagacggcaa
      121 cggagcggat accaagttcg cggcaagcgc gcacgatgcg cacggcgatc tcaccgcgat
      181 tggcgatcag gattttccgg aacatgcctg gtttgcgatg cagcggaatt ttagcacgcg
      241 tagcggggag gcgacgcttg cggtttggct tgcgtgtcca atcgtttgcg gtgtaagaag
      301 gtgcgggacg aatgcgggta gaggacaact atgaagaaac tgaatctgtt tttcgcagcg
      361 ctgttgttga tggcggtaag cgcgccggcg caagaggcag tcatttcgcc cgccgacaat
      421 tagatcgtgg atggggtgcc gaagataccg gcatcgttgg cggagaccgc ggggcgatac
      481 ggttcgtatc gaggtgccag tctggcggat tggcatccca cgaagcgaga gatgctgatc
      541 gcaacgcggt ttgcggagac gcctcagctt catctcctga aaacgccggg tggagcgcgg
      601 caacaactga cattttttgc agactccgtc ggcggcggaa ggtttcatcc gaatggcggg
      661 gactacattc tgtttgcgaa ggatatcggc ggcggggagt ggtaccagct ctatcgttac
      721 gatatggcta ccggcgatgt gacgcttctg acggacggga agtcgcggaa tttgccggga
      781 ccgtggtcgt cgagcggcga tcagatcgcc tatatgtcca cccggcgcac cggaaaagat
      841 acggatttat gggtaatgaa tccagctgat ccaaagtcgg atcatttgtt gaaggaactg
      901 gagggtgggg gctggcaacc gctggattgg tcgccggatg acaaaaagat cctgctggaa
      961 gaggaattgt ccatcaatga gtcgtatctc tggctggtgg acacaacgac aggcgagaag
     1021 acggagctaa cgccgaggaa tacgaccgaa agagtttctt acggcgaagg acggttcagc
     1081 aaagacggaa aaggcattta cgtcactacc gacaaggctt cagagattca cagacttgcg
     1141 tatgttgacc ttgaatcgaa acaacactgg tatctcacca gtgagatccc ttgggatgtg
     1201 gatatgtttg acgtctcgca agacggcaag cggattgcat tcgtgacgaa tgaagacggc
     1261 ttgagcgttc tgcgctggtt cagcacggat cacaaaccag ttgtcgttca aaaatcgccc
     1321 aatggagtaa tcggtggagt gcgctggcat aagaacggac gggagctggg gttttcgctc
     1381 gcaaatgcgc aggggccggg ggatgtgtac tcagtggatg taaccacttt aaagatcgag
     1441 cagtggacga ccagtgaaac ggcagtgaag acaggtgctt ttccgcaggc agcgttggtg
     1501 gagtggaaga gcttcgacgg aaagacgatt tcgggattct tgtacaagcc gccggcgaag
     1561 ttcaccggca agcggccagt gctggtggtg attcatggcg ggcccgaggg gcaatcgcag
     1621 ccgacatttc tcggacgagc caattacctg ctgaacgaac tggggatcgc gctgatctat
     1681 ccgaacgtgc gcggttcgac cgggtacgga aagacgttct cgctgctgga taacgggttc
     1741 aagcgcgagg atacgtacaa ggacatcaat gcgctcttcg actggatcgc gacgcagccg
     1801 ggactggata gcgagcggat cgcggtgacc ggcgggagtt acggcggaca catgacgctg
     1861 gctgtctcga cgttttacag cgaccggatt cgctgctcgg tggacatcgt gggcatgtca
     1921 aacctggtga cgttcctcga gcacaccgag gcgtaccgaa gggatttgcg gcgcgtggaa
     1981 tacggcgacg agcgcgaccc gaagatgcgg gagttcctcg agaaaatcgc gccgatgaac
     2041 aacatcgaga agatcaagaa acccatgttc gtgattgccg gaaagaacga tccacgggtg
     2101 cccgtcagcg aatcgcaaca gatcgccgac gcgctgaaga agcaggggac tcctgtatgg
     2161 ctcctgatcg cgaaggacga aggccacggg taccgaaaaa aaccgaacca ggattttcaa
     2221 ttctacgcga cggtcgagtt cttgcaggag tatttgctga agtgaccgca cgatgagcgt
     2281 cgtcgtcctg agtgtagcgt gaatcagtac cagatctcca tgctcaagcc gcgagtatcg
     2341 gattctgtgc tttgatgtgg cacgcgaaaa gtcttttcgt tgagcgcgtt agccgcgacg
     2401 gggatcccga ttagcgcgtc aagtatatcg tcaatcgcag cggctcgacc taaacgccgc
     2461 tcccactcaa gcgcttcatt gaggccagat atataagaac cgacaatctc cgtgcgttgt
     2521 gaaaggccac ttttttgatg ctttgagtca agtactcgtc ccgcagctcg ccaccacgca
     2581 agttccgggt gaaattcatg gattctcttc tgaagatcag gggtcatgat ttcgtcgact
     2641 tccttcaact ttctcacgat gccccacacg ggccagccag cgccctttcc acgaaatttg
     2701 ctgtgtagta tttggaattc ttgcggatca ctagcgtgca tagcctcccg cggaggagca
     2761 agaaatacgc gcgtactccc ttctctcccg agaagccttc tcgcatcttc gtcgcaggtg
     2821 cgaatcttct ttcccgacgg caatcctatg ggaatgtcaa caacgacctg ttcgcaatca
     2881 agggtggact gaacgaggga tcggaagtcg gcgcatatcg agaggaagag aggctctttg
     2941 caaggccacg acttactctt aaccagtaac cagccccctt tacaaccgtc agcgctcgcg
     3001 agcatggtca cctatagcgg gatgttgccg tgtttcttac ggggattcgt gtcgcgtttg
     3061 ttttcgaggg cacgaagaga agtgataatg cgggcgcggg tatcggctgg ctcgatgacc
     3121 gcgtcgatgt agccgcgctc cgcggcgacg aaaggattgg caaagcgctc gcggaactca
     3181 gcgatttttt cctggcggag tttttcgcgc tcggattcgg gagctttgtc gagctcgcgc
     3241 ttgtggacga tgttgaccgc gccttcggga cccatgacgg cgatttccgc ggtgggccag
     3301 gcaaaattgg cgtcggtgcg aatgtgcttc gaggccatga cgcagtaggc gccaccgtaa
     3361 gccttgcgcg tgatcaccgt gattttcggg acggtggctt cggcgaaagc gaagagcagc
     3421 ttggcgccgt gcctgatgat tccgccgaat tcctgctggg tgcccggaag aaaaccggga
     3481 acatcctcga aggtgatcag cggaatgttg aaggcatcgc agaaacggac gaagcgcgcg
     3541 cccttcacgg aagcgttgat gtcgagaacg ccggcgagga aggccggctg attggcaacg
     3601 ataccgacgg gccggccgtc gaggcgcgca aagcccacaa caaggttctt cgcgtagtgt
     3661 tcgtggactt cgaaaaaata accgtcgtca acaaccgagt ggagaacgtc cttgatgtcg
     3721 taaggcttct gaggatcggc gggaacgaga gtgttgagcg attcatcgcg gcgagccggg
     3781 gagtctgatg tggcgcggcg cggtggatcc tctaggttgt tggaagggag aaaactcatc
     3841 aactcgcgga tcatggccaa gcaatcggca tcgtcacgcg agacgaagtg ggccacgccg
     3901 ctggtggcgt tgtgggtcat cgcgccgcca agctcctgct tggtgacttc ctcgtgcgtg
     3961 acggtcttga tgacgtcggg gccggtaacg aacatgtagg aggtctcgcg agtcatgaag
     4021 atgaaatcgg tgatggccgg agagtagacg gcgccgccgg cgcaaggacc catgatggcg
     4081 ctgatctgcg ggatgactcc gctggcaaga gtgttgcgca aaaaaatatc ggcgtaacct
     4141 gcgagcgagg cgacgccttc ctgaatgcgc gcgccgccgg aatcgttgag gccgatgacg
     4201 ggagccccgg cgcgcatggc caaatccatg atcttgcaaa tttttagcgc gttggtttcg
     4261 gaaagcgaac cgccgaaaac ggtaaagtcc tgggcgaaaa cataaacgag acggccatcg
     4321 atgcggccaa aaccggtaac gaaaccgtcg ccggcggggc gctgctcctg catgccgaaa
     4381 tcgacgcagc ggtgacggac aaatttgtcg agctcttcga aagtgccttc gtccagcaaa
     4441 agatgaatgc gctcgcgggc agtgagcttg ccggctttgt gttcgcgctc gcggcgctcc
     4501 gggccgccac cggcctcggc ttcgtcggaa atacggcgaa gctcttccat acggtcgcga
     4561 tgtttgtctt cgggaaccaa actgggcttg gtctcagcca acgggtcctc ccccggaagg
     4621 agattccttt ggggtaaaaa tgcgcacggc cagatgcgtg gcgagccaga cggttcccag
     4681 gagcagcacg actcccagga gcagctccgc acccatggcg acggcgcgct gcagaggaga
     4741 ttcgagcgcc gtcacgtact ccgtggcccg aaccagaatg aacatggccc cgagggtcat
     4801 tgccacgatg aggatggtgg ttaagaagga agcgataatc attttcatcg gactggaact
     4861 gggccacccg gaagcggcat ggagaaggat aacgcaacgg gagggaaagc agaacgcaga
     4921 agacggccgg ttagcgggag agcaattggc gcaagtgatt agactctcag cgtaagcccg
     4981 accagtcgtc ggcgacgagg atggaatcgc tgggattggc ggcgtcatac ttgatgctgg
     5041 cgggctggcc ggcgacgacg cgctcaagcc gaatctggct ttcgaggccg gtgatgtcct
     5101 gcgcggtctg ataggtaacg ccggaaatca aataactata ggagacgaga tggcgcggcc
     5161 gcatgtcgct tagcgggcgg gcgccagcgc cgaacaaacc cttgcgcgcc tctttcgatt
     5221 cgggcgggtg ctcgacgagc tcgacgatct gcccttcggc tatgcggccg atctgattca
     5281 ggtgcaggcg gcgcttgcgc tcctcttctt cgggattcac agcgggacgt aagaaggcat
     5341 aagcgatcat ggccagcgcg gcgatcgccg cgccacccat ggcgagcaca actttccaat
     5401 cggcaagcaa gtccttcaat gagctgactc cataatctca aatgcgcggg attgcgcatg
     5461 gcatgtgaaa gatggcgtgc acgcagtgcg gcggaaggat ttcctccacc cgccgatgcc
     5521 ccactgtgcg tattctaccg aaaccccgag gcatacgtga aactacgctt gtggagcgtg
     5581 caccggattg cgcaacacgc caacaccggc aacgctaacc tccaccgtat cccccgcaac
     5641 cagcgggccg acgccggcgg gcgtaccggt ggcgatgacg tcgccaggca tcagcgtcat
     5701 catgcgagaa atccagcgca cgagaaacgc aggcgaatag gtcatcagcg aggtggcgcc
     5761 agactggcgg cgcgcgccgt tgacgtgcgt ctccaccaga acactggatg gatcgagagc
     5821 ggtttcgatg tgcgggccga cagcgcagaa agtatcaaac cccttgccgc gagtgaattg
     5881 aacatcgctc ttctgaaggt cgcgcgcggt gacatcgttg aggcaggtgt aacccaccac
     5941 gaaggagaga gcatcttcgg catcgctgag ttgcgagcaa cgccggccga taaccagcgc
     6001 cagttcgcct tcgtgttcca cgcgttgcga atattttgtc aggacgatcg cttcttccgg
     6061 accgatgatg gaagagggcg gcttaagaaa catcaacggc tctttcggga cttcgttgcc
     6121 catctccgcg gcatgggcgg cgtaattgcg gccgatgcaa acgatcttcg aaggcgccac
     6181 tggagcgagc agccgcacat cggcgaggcg cgaagtacgc gagccgcggg accagggacc
     6241 ccagggcggg ccagagatct cgatgacatt ttctccttcg atcagtccat agcgcggcga
     6301 agaagagtcc gaagaggtag cgtcgcgcat ctggtaacgg cagattttca ttggtagtta
     6361 actcgcatgg tggttgggat gagaagcgtg cgtcccacga gccctccaga tagtggcaac
     6421 aatccaggag agcgtcgtca gaatcaaata caccacggcg cccagcataa aaacctggaa
     6481 cgtactgagc cgattgctga ggccgtggaa gatcaggcag agcgcaccat agctcaggct
     6541 gaaaatcagc cagccaagaa ggctcgatgc caggagatgc cccggcaacc gcagaaacct
     6601 aaaaatcgga accgcagcga ggaagccgaa aatcgcggcg gcggcgatgt tgcgctccat
     6661 cgcaaagcgt tccaggaatg gggcatgatt ggctatgacc agccacgcgg tgaaaaccaa
     6721 agtcagacaa acacccacgt aaacaccggt gcgaatcgcc gcgttgcgga agacatggaa
     6781 ccaacgataa ccgggcagat gcggacgtac cagcatgaag tgctcggctc ccaagatgcg
     6841 agcaatcgtt caccccggat ttacggcgat ggtgacgtga cgtcgacagc gaccggtgcg
     6901 ccgggcgcgc tttcattccc cgcgcggtct acagcggtca cggtgtacca atagcggtgg
     6961 ccgggctcta cagacgtatc acgaaccgca ggcgtgggca acaagtccgc gcctagaagc
     7021 tggccccttg tgccctcctg ctcacttcta tagacgcg
//
LOCUS       DASYVO010000460         7543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_27795, whole genome shotgun sequence.
ACCESSION   DASYVO010000460 DASYVO010000000
VERSION     DASYVO010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7543
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27795"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..753
                     /locus_tag="VGR03_15730"
     CDS             283..753
                     /locus_tag="VGR03_15730"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEV1995782.1"
                     /translation="MAYNVFISYSSKNLHIVEWARATLAQPGITEVFAAEYSVLPSQV
                     LNDEIVRAIRACDVFVLLWTHDARASDYVPQEIGIAVGCNKTILPVVMEDNVPVPGFI
                     TNLKYLPAHKDWDASFQWLTQFVHDNAEKLKNAKALGALAAIILGAIWLFGDHD"
     gene            906..1499
                     /locus_tag="VGR03_15735"
     CDS             906..1499
                     /locus_tag="VGR03_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995783.1"
                     /translation="MSVQTELDSARRLGAQLEELVVKRGECPDDDRNILLMGYWALLF
                     DFHKGVLSLIGNALCGSAFALVRPSTEALVRAHVAVKGSADDIKNLQQDDYRTNFATI
                     GLWIDTEFGTGDLFTKFLGHSRDALHSFTHAGISQLGRRFQGHDLTPTYDDGEIIEVI
                     RVCTSAVWMVTNLVTKHLGFADEAKKAQELYLEWGKH"
     gene            1699..2073
                     /locus_tag="VGR03_15740"
     CDS             1699..2073
                     /locus_tag="VGR03_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995784.1"
                     /translation="MAVVMEPVATVRKTRQKIKRLQAKLTGTSVEEEHGQHLILGTSL
                     NAGVGAVEIALYASSVVFGHPEFIAVWFATKYVAFFRSWGKEPVGRTFYNRSLIGSGL
                     NILLGFFTGKFALWAISHFGGS"
     gene            complement(2082..2714)
                     /locus_tag="VGR03_15745"
     CDS             complement(2082..2714)
                     /locus_tag="VGR03_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995785.1"
                     /translation="MADELPSGDKAVYIYLEFIALGFALEAVAAFVRGDGWHIWAGSI
                     VLSAVFLLAGIKWPWIKGKFVSVDWVLWDKRLNRALLVVLVLGFVTVAATGYYTWRKT
                     SLAWSQFSREFSRLLGRIRTVPKEEVAKSKNTPAVPSTERLDWSDKQNWRRFLRHGMT
                     RTEVRRLFGDPEKMAVVSDMEFWDYGTGQIDFDMEDHPDGSLYSWFEPGP"
     gene            3765..4973
                     /locus_tag="VGR03_15750"
     CDS             3765..4973
                     /locus_tag="VGR03_15750"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEV1995786.1"
                     /translation="MSEQIGELLPPTRLMMTPGPSSMDPRVYRAMSTPLVGHLDPWFR
                     GLMDEIQQLLRAIFRTENRVTFPLSASGSGGIEAAVLNPLEEGDEALICVNGTFSERM
                     AIIADRTAAKITRIEAPYGKPVDPEDVRRAGKGKKVKIVGLAHGETSTGVITNIDAFR
                     KVADELDAMLVVDTVATLAGVPLGVDRQRIDICFSGTQKAISAPPGMAPITVGPRAEE
                     VLRARKTKVQSWYFDLTSAMNYWGKERLYHHTPPISLIYGLREAMRIVLEEGLEARWE
                     RHRQNQQALMAGIEAMGLQFFVKNPVDRLPTVTAVLVPEGIDDLRVRTQLLDEFNIEI
                     AGGFGPVKGKIWRVGLMGYVSQKKNVLLFLAALEKVLLEQGFRAPAGAGAGAAVKSYL
                     EAATAAVAGR"
     gene            5013..5384
                     /locus_tag="VGR03_15755"
     CDS             5013..5384
                     /locus_tag="VGR03_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995787.1"
                     /translation="MSKPLLANVGPMNQFIGVFEVTSRLTGKVYQCRFSHMWNGIATR
                     HSDTIDTKFFVDGAGVVLGLAHTAFVEFREKNGRDLTDREASYIAAEYLRERLEEEDE
                     RALYDVSHSDVARLIPQLELR"
     gene            complement(5407..6651)
                     /locus_tag="VGR03_15760"
     CDS             complement(5407..6651)
                     /locus_tag="VGR03_15760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1995788.1"
                     /translation="MTENSHSLTAYQLRVLAVLALINFVNFADRQVIVPLVPLLRDQL
                     GVTDAQLGSLQTWLLVILAVASIPSGFLADRISRKRIIAAGVIFWSLATMASGLALSF
                     TFLLVARALVGVGEAAYAPAAQSMISGAFPRESRARALAVFASGMLLGGASGLALGGV
                     VGELRGWHYVFILVGAAGLVPGLAILRLAEPPRPPRSEVVPLSHLLRVPAFLAMIAAG
                     MFITFSSVSLLAWGIDFAVNYKDFSLREAAVSLAFTTFFSLVLGVLAGGYVADRLQKK
                     FVYGRIIAIAGAFLFAAPFVLLAIESDTKWVVLGALSIAGFFMSWYHGPVTAVIHDMM
                     PQRAHASSIGVYMFATQLVGASGPQLVGKISDMRDLQIGLRVAVGVMVGGALLMFLVI
                     YFIRRDGLHHPQLESFRTEQPD"
     gene            6723..>7543
                     /locus_tag="VGR03_15765"
     CDS             6723..>7543
                     /locus_tag="VGR03_15765"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1995789.1"
                     /translation="MDNSPAAKANDRVTTVLSYGVLLLLIYLVYRIFEPFLTALGLAC
                     VLVVFFFPLHLRLKKRLGNTRAAAVSTAGVTLLLIVPAIILATLFAREALSLSRGLEK
                     VLIEEQAPALTHAWEWIARHVPGMNPNLNLIELFRETLQKKAGFLAAQLGTALRNLAT
                     FVFDLFVMLFAIFYLFRDAEEVMRGVRHILPFDTERRDAILEQARDLISASVTTSLIL
                     GVIQGVLGGVAFAVVGLANPVFWGVTIGFFSLLPVVGSGLIFVPAALWLGLGGHWG"
BASE COUNT         1631 a         2184 c         2246 g         1482 t
ORIGIN      
        1 cccgctcccc cacccgatcc ccccaccgac cacgtcattt gcgcctttgg catcccggac
       61 ggttctgcgg gaccgagcgc agcgagccct ctgccccatc ccgcgcgtcg aacagacccg
      121 ccgcaggcgg gcgggatgaa atctctcttc gttcagcgcc cttcatttct tcacaccgca
      181 gtgaattaca gaagcggtat actctcgttg cgtcaagccg gcaacaaata gagatttttc
      241 ggaccatccg aagccgacca agaggtaggg aggacccaag cgatggcata caacgtgttc
      301 atcagctatt cgagcaagaa cctgcacatc gtggagtggg cgcgcgcaac attggcacag
      361 cccggtatta cagaagtttt cgcggccgag tactccgtac taccgagtca ggtcctaaat
      421 gatgaaatcg tccgggcaat ccgcgcctgc gacgtcttcg tactgctttg gacccacgac
      481 gcccgtgcct cggattacgt gccacaggaa atcggaattg ctgtaggctg caataaaacg
      541 attcttccag tggtcatgga agataacgtc ccagttcccg gcttcataac taacttgaaa
      601 tatcttcctg ctcacaaaga ttgggacgcg tcttttcagt ggctcacgca attcgttcat
      661 gacaacgccg agaagctgaa gaacgccaag gcgctcggtg cgctcgccgc gatcatcctt
      721 ggcgctatct ggttattcgg ggaccacgac tgagacgagg aggggtgaca acgcgaatag
      781 cagtggaccc ttgcgctgcg cccactcagt ctttggtctt cggcttgccg tgatcggccg
      841 caagctcacc gaaaaggaag aatgcgtctc atgcgagggc aagtgcaagt cctggaaacc
      901 gagcagtgag cgtccaaacc gaactcgaca gtgcgcgccg ccttggtgcg caacttgagg
      961 aactcgtcgt aaagagaggg gagtgccccg acgacgaccg caacattcta ctgatgggat
     1021 actgggcact gctttttgac tttcataagg gagtcctgag cctgatagga aacgccttgt
     1081 gcgggagcgc ctttgcgttg gtccgcccga gcacggaggc attagtgcgt gcgcacgtcg
     1141 ccgtaaaagg ctcggcggac gatataaaga acctccagca agacgattac cgcacgaatt
     1201 tcgccacaat cggcctgtgg attgacacag aatttggaac cggagattta tttacaaagt
     1261 ttctgggcca ctccagggat gcgctgcata gcttcacgca cgccggtata tctcagctag
     1321 gaagacgttt tcaaggccac gatctgacgc cgacttatga cgacggcgaa atcattgaag
     1381 tgattcgcgt ctgcactagc gcggtgtgga tggtcaccaa tcttgtcaca aagcatctag
     1441 gattcgccga cgaggcgaaa aaggcccaag agctatatct tgaatggggc aagcactaga
     1501 cggattaaag agcatcaggc ccgaatctca cgattcgggc atcggtagtg tgttaggatt
     1561 tcttcatgtg gtccggtgct atggtagtta gcgcggtgcg ctatcagcat aatgccgcat
     1621 aacatctgtc aatggtctgc ctacgttttc gttgtaaccg tagggatctt cgtctcttgg
     1681 gggattcagt ggttactcat ggcagtggtc atggagcctg tagcgacggt tcgaaagaca
     1741 cgtcaaaaga ttaagcgtct ccaagccaaa ttgacgggaa catcggtgga agaggaacat
     1801 ggccagcatc tcatcttagg cacaagcctg aacgcagggg tcggggcagt tgaaatcgct
     1861 ctttacgctt cgtccgtcgt attcggacac cccgaattta tcgccgtctg gttcgctacc
     1921 aagtatgttg ctttcttcag gtcttggggt aaagagccgg tggggagaac cttttacaat
     1981 cgctccctca tcggtagtgg actcaacata ttgctcggtt tcttcacagg taagttcgca
     2041 ctatgggcca taagtcactt cggtggcagc tagcatgagc gctacggccc tggctcaaac
     2101 cacgaatata gacttccgtc gggatggtcc tccatgtcaa agtcgatctg acctgtcccg
     2161 taatcccaaa actccatgtc agataccacc gccatttttt caggatcccc gaaaagccga
     2221 cggacttctg ttctcgtcat cccatgtcgt aagaatcgcc gccaattctg tttgtcactc
     2281 caatccagac gttcagttga aggcactgct ggagtattct tggatttcgc cacctcctct
     2341 ttggggacgg tccgaatgcg ccccaaaagt ctagaaaact cgcgagagaa ctgactccac
     2401 gcaagagatg tcttccgcca cgtataataa cccgtcgccg caaccgtaac gaagcccaag
     2461 acaagaacta caagcaacgc acggttgagc cgtttatccc aaagaaccca gtcaacagaa
     2521 acaaatttgc ctttgatcca tggccatttg ataccagcta gcaagaacac ggctgacagt
     2581 actatcgatc ctgcccagat atgccatccg tctccacgca cgaaagcagc gactgcttca
     2641 agagcaaaac ctagtgcgat gaattcaaga tagatgtaaa cggctttatc gccggatggc
     2701 aattcatcag ccatgagtag atccctcaaa accgtgctcg ggattttaga ggacagttta
     2761 caaccgctag tctgacttcc tcggtattct tttcggtatc tttaagaaat ggcaagcgga
     2821 aggattttca aactggccca ctacccagtc gtcaacgact ccccagctcc tccgggacct
     2881 ctgccccctc cctccagaat caagcccctt gtccccaacc tcttcccctt caatgactta
     2941 tagaaaatac ttgacaatga gctaactctt gtggtaacat tccttgtgag ttcgatgagc
     3001 tttcagtccc gcccggcggg acgaatcctg agcgcagggc cgctctctcc cgcccgtagc
     3061 cgaagggcgc tacaggtcgc tgtaggcctt ccgctcccca cctcattgcc ttccctgctt
     3121 cgacatttcc ccctgcaaag gcgcttccct aaccgagcat ccgaccagga tgcgactccc
     3181 gaggagcatc gcgacgaggg atctctcttc cgagctaacc cctctggaat caacacttgc
     3241 aaaagtgtat caaaacaaag aacttcatcc ccctttagaa tcaacactta caaaaaacag
     3301 gggggagggg gagtatctct tctgtcaacg gttccggtac ccgcttccca gccgatcaca
     3361 aaccggccac ccgttgccac ccatccttcc ttcacttcct ctaccccatc tatctccttt
     3421 atttccttca ttttgcgcac tctttttact cttgccgcgc gcaggaatcg ccgcaacccc
     3481 tttgccctca agcgtttccg cagtcttgtg aaaacaatag ggggtggggg gataccgctg
     3541 tgggaatctt tctttctcct ggagcttttc gtgaactgtc agcccttact ttcaaccttc
     3601 aactttcaac tctcaacctc cgctcggatg ttcccaagcc ctccaatttg tccgttgcag
     3661 gcatggcgcg gatgggccag aattgcctct cctgtcctcc agctccgagc ggcggaacca
     3721 ccagccccgc cgcagtctgg cgggcagcga ccgaggcgaa actcatgagt gaacagatcg
     3781 gcgagctcct tccccccacc cggctaatga tgacgcccgg gccatcctcg atggacccgc
     3841 gggtctatcg ggccatgtca acgccgctgg tggggcacct cgacccgtgg ttccgcggcc
     3901 tgatggatga gattcagcaa ctgctccgcg cgatcttccg cacggagaac cgcgtcacgt
     3961 ttcccctgtc ggcgtcgggc tcgggcggga tcgaagccgc cgtcctgaac cctctcgagg
     4021 agggcgatga agccctcatc tgcgtcaacg gcaccttctc cgagcgcatg gccatcatcg
     4081 cggaccgcac tgccgccaag atcacgcgca tcgaagcgcc ctacggcaag ccggtggacc
     4141 cggaagacgt acggcgcgcc ggcaaaggca agaaagtgaa aatcgtcggc ctcgcgcacg
     4201 gggaaacctc gaccggcgtc atcaccaaca ttgacgcatt tcgcaaagtt gccgacgagc
     4261 tggatgccat gctcgtggtg gacacggtgg cgacattggc gggcgtgccg ctgggcgtgg
     4321 accgtcaacg gatcgacatc tgcttcagcg gcacgcagaa ggccatcagc gcgccgccgg
     4381 gaatggcgcc gatcaccgtc ggcccgcgcg cagaggaagt tcttcgcgcg cgcaagacca
     4441 aggtgcagag ctggtatttc gacctcacct cggcgatgaa ctattggggc aaggagcggc
     4501 tctaccacca cacgccgccg atttcgctca tctacgggct gcgcgaagcc atgcgcatcg
     4561 tgctggagga agggctcgaa gcgcgctggg aaaggcaccg gcagaaccag caagccctga
     4621 tggccgggat cgaagcgatg gggctgcagt tcttcgtcaa gaatccggtg gaccgcctgc
     4681 cgacggtcac cgcagtgctg gttccggagg gaattgacga cctgagggtg cggacccagt
     4741 tgctcgacga attcaatatc gagattgcag gcggattcgg ccccgtcaag gggaagatct
     4801 ggcgcgtggg attgatgggc tatgtgagcc agaagaaaaa cgtactgcta ttcctcgcgg
     4861 cgctcgagaa ggtgctgctc gaacagggct tccgcgcgcc ggccggcgcg ggcgctggcg
     4921 cagcggtgaa gagttacctc gaggcggcga cggcggcggt cgctggccgc taagggccgg
     4981 gcgctggaag ctcgaagaga ggaagccgcg tggtgtcgaa gccgttgctg gcgaatgtgg
     5041 ggccgatgaa tcaattcatc ggcgtctttg aagtgacttc gcgcctgacg ggcaaggtct
     5101 atcagtgccg gttctcgcac atgtggaacg gcattgcaac gcggcacagc gacacgattg
     5161 atacgaagtt cttcgttgac ggcgccgggg tggtcctggg gctggcgcac acggcgttcg
     5221 tggaattccg cgaaaaaaac gggcgcgatt taaccgaccg cgaagccagc tacatcgcgg
     5281 cggagtatct gcgcgagcgg ctcgaagaag aagacgagcg cgcgctttac gacgtgtcgc
     5341 actccgacgt ggcgcggctg atcccgcagc tcgagctgcg ctgaagctct cactcttgga
     5401 aatggttcag tccggttgct cggtgcggaa tgattcgagt tgcgggtgat gcagaccgtc
     5461 gcgccggatg aaatagatca ccaggaacat cagcagcgcc ccgcctacca tgactcccac
     5521 ggccacccgc aagccaatct gaagatcgcg catgtcggag attttgccga cgagttgcgg
     5581 cccggacgcg ccgacgagct gcgtggcgaa catgtagacc ccgatggagg aggcgtgggc
     5641 gcgctggggc atcatgtcgt gaatcacggc ggtgacgggg ccgtggtacc aggacatgaa
     5701 gaatcccgcg atggacaggg ccccgagcac gacccatttt gtgtccgact caatggcgag
     5761 gagcacgaag ggcgcggcga aaaggaatgc tccggcgatg gcgatgatgc ggccatagac
     5821 gaatttcttc tggaggcggt cggcaacgta accgccggcg agcacgccga ggacaagtga
     5881 gaaaaacgtg gtgaatgcga gcgagacggc ggcttcacgc aggctgaaat ccttataatt
     5941 gacagcgaag tcaatgcccc aggcgagcag cgaaacgctc gagaaggtga tgaacatgcc
     6001 ggcggcgatc atggccagaa aggcgggaac acggaggaga tgggataggg ggaccacctc
     6061 tgaacgcggc gggcgcggag gttctgccag gcgcaggatc gcgaggccag gaacgaggcc
     6121 ggcggcaccg acgagtataa agacataatg ccagccgcga agctcgccca cgacgccgcc
     6181 gagggcgagg ccggaggcgc ctccaagcaa catcccggag gcgaaaacgg ccagggcgcg
     6241 ggcgcggctc tcgcggggga aggctccgga gatcatggac tgcgccgcgg gggcgtaggc
     6301 ggcctcgccg acacccacga gggcgcgggc aacgagaagg aacgtaaagg agagagccag
     6361 gccgctggcc atggtggcca ggctccagaa aatgacgccg gcggcgatga tgcgcttccg
     6421 gctgatgcgg tcggccaaga aaccggaggg aatactggcg acagcgagga tgacgagaag
     6481 ccaggtctgg agggagccga gttgcgcgtc ggtgacgcca agctggtcgc ggaggagcgg
     6541 aacgagcggc acgatgacct ggcggtcggc gaagttgaca aagttaatca aagcgagtac
     6601 agcgaggacg cggagctggt acgcggtcag agaatgagaa ttttccgtca ttcgaaaagg
     6661 cttccgggag ctgcggcaac ccagtcctgg aaacgctgac tgattatata ccgagggtga
     6721 ccatggacaa ttcgccggcg gccaaggcca atgaccgagt gaccaccgta ctgtcgtacg
     6781 gggtgctgct gctgctgatc tatctcgtct accggatttt tgagccgttt ctgacggcac
     6841 tggggctggc gtgcgtgctg gtggtgtttt tcttcccgct gcacctgcgt ttgaagaaaa
     6901 gactggggaa tacacgcgcg gcggcggtga gcacggccgg ggtgacgctg ctgctgatcg
     6961 tgccggcgat tatcctggcg acccttttcg cgcgcgaagc cctgtcccta tcgcgtggac
     7021 tcgagaaagt gctgatcgag gaacaagccc cggcgctcac acacgcgtgg gagtggatcg
     7081 cgcggcacgt gccgggcatg aacccgaatc tgaacctgat cgaactgttc cgggagacgc
     7141 tgcaaaagaa ggcgggattc ctggcggcgc aactcggcac ggcgctgcgg aacctggcga
     7201 cgtttgtctt cgacctcttc gtgatgctgt tcgcgatctt ttatctgttc cgcgacgccg
     7261 aggaggtcat gcgaggcgtc cgccacattc ttcccttcga tacggaacgg cgcgacgcca
     7321 tcctggagca ggcgcgggac ttgatctccg cgagcgtgac cacgagtctg atcctcggag
     7381 tcatccaggg agtcctggga ggtgtggcct tcgctgtggt gggactggcg aatccggtct
     7441 tctggggcgt gacgatcggg tttttctccc tgctcccggt ggtgggttcg gggctgatct
     7501 ttgtgccagc cgcgctgtgg ctggggctcg gcggccactg ggg
//
LOCUS       DASYVO010000461         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_148859, whole genome shotgun sequence.
ACCESSION   DASYVO010000461 DASYVO010000000
VERSION     DASYVO010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148859"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..756
                     /locus_tag="VGR03_15770"
     CDS             55..756
                     /locus_tag="VGR03_15770"
                     /inference="COORDINATES: protein motif:HMM:NF024197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HEV1995790.1"
                     /translation="MAGLRNAGGRSQARSTLGRFLRFGVLRGLRTIEIDPEDFRRELS
                     DKHGLWVPDFARMKDVPVERLDAIAKKLIRDAERLALAEGAGFGLGGMITILPDASLL
                     TLITLRLIQRLSLLYGFEAHGRDQRMEMWKAAAAASGIDYGKDLAEKQILEKVAPRIA
                     ERLAMKIGAETAEKWIGRLIPLASSAVGGALNFSFVRGWGRRVQRQLRAKHVGAKAST
                     PPPPAAYTAAEAALV"
     gene            778..1758
                     /locus_tag="VGR03_15775"
     CDS             778..1758
                     /locus_tag="VGR03_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995791.1"
                     /translation="MPAPAPEGFLLGVNYPWVHYGQDFGRSLSGVHGVSTSETRKAVA
                     QDFERMRAAGITLARWFLLCDGRSGLSVNKGIPTGPDELLFADVAAALELARQFELRL
                     CFSLLDFLWMQNRGEASSSLPNQNVLKFAAGREALLEHVLIPLFEEFRGHPALFSWEV
                     ANEPEWAIREFVRFPEAVMHFADFNAFAREIADAMHEYSHAQVTLGSARLLWFRAWSD
                     LGLDFYQAHYYPQNERDQDADLAAQLRALKPQESLDRALWLGELPARDPGTPGYTLEE
                     ALDTCKAAGLSGAGIWRWRNPDPGSADEKYGAADPDILRTWLTRSGASSV"
     gene            1876..>2434
                     /locus_tag="VGR03_15780"
     CDS             1876..>2434
                     /locus_tag="VGR03_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995792.1"
                     /translation="MKGTPAEPADAADVREQIAIVEKLKSIVPDRGAVLYFLSTAKQH
                     LGETREALALLKECLALREGFDPSGEAAFLGLKGTKEFDDLVASVHRDFPAAAHARLA
                     FVTDEKDLIPEGLTHDAKQNLFYLSSQYRRKIVKIDPQGRVSDFVPGDRYGLLPVLGI
                     RLDASDGTVWADTFEDAGRTEIVHFD"
BASE COUNT          464 a          677 c          761 g          532 t
ORIGIN      
        1 ggtgcaacgc tgtggttgcc agccggttct aaccgccgta ggatgctaat agacatggct
       61 gggttacgga atgcgggtgg aagatcgcag gcgagaagta ctctcggtcg cttcttgcga
      121 ttcggtgtac tccgcggcct gcgcacgatc gagatcgacc cggaagattt ccggcgcgag
      181 ctttcggaca agcacggcct gtgggtcccg gatttcgcgc gcatgaagga tgttccggtg
      241 gagcgcctgg acgccatcgc gaagaagctg attcgtgatg ccgagcgtct ggcgcttgcg
      301 gaaggcgcgg gatttggcct gggtggcatg attacgatcc tgccggatgc cagcctcctg
      361 acattgatca cgctgcggct gatccagcgg ctttcgctgc tttacggttt cgaagcgcac
      421 ggacgagacc agcggatgga aatgtggaag gcggccgccg ctgcttccgg tattgactac
      481 ggtaaggatc ttgccgaaaa acagattctc gaaaaagtcg ctccacgcat cgcggaacgc
      541 ctggccatga agattggggc ggaaacagcg gagaaatgga ttggccggct gatcccactc
      601 gccagttcgg cggtcggcgg agcgctgaac ttttcctttg tgagaggctg gggccggcgg
      661 gttcagcggc aactccgcgc gaagcatgtt ggagcaaaag cttccactcc gcccccgcca
      721 gcggcgtata ctgcagccga ggctgcgctc gtttaacgac cgcagtttta gagcaacatg
      781 ccagcccctg cacccgaagg ctttctgctc ggtgtgaact atccctgggt ccactacggc
      841 caggattttg gccggagcct gtcgggtgtg catggcgtca gcacttcgga aacgcgaaaa
      901 gcggtcgccc aagatttcga gcgcatgcgg gcagcaggaa tcacgcttgc acgctggttt
      961 cttctctgcg atggacgcag cggcctctcg gtgaacaagg gaattcccac ggggcccgat
     1021 gagctgctct ttgccgatgt ggccgcggcg ctggagttgg ctcggcaatt cgaattgcgc
     1081 ttgtgcttct ctctgctgga tttcttatgg atgcagaatc gcggtgaagc ttcgtcctcc
     1141 ctgccgaacc agaatgtctt gaaattcgcc gcggggcgtg aagcgctcct cgagcatgtc
     1201 ttgattccgc tctttgaaga atttcgcggt catcccgcct tgttctcatg ggaagtggcc
     1261 aacgagccgg agtgggccat tcgtgaattc gtgcggtttc ccgaagcggt gatgcacttt
     1321 gcggatttca atgcatttgc ccgcgagatc gcagacgcca tgcacgaata ctcgcacgcc
     1381 caggtgacgc ttggttcggc gcggcttttg tggttccgtg cctggagcga cctgggactc
     1441 gatttttacc aggcgcatta ttatccgcag aatgaacgcg accaggatgc ggatctcgcc
     1501 gcgcaactgc gcgctctaaa gccacaggaa tctcttgatc gcgcgctctg gcttggagag
     1561 ttgcccgcgc gcgatcctgg gacacccgga tacacgctgg aagaagcgct ggatacttgc
     1621 aaagccgccg gtctctcagg cgcaggaatc tggcgatggc gcaatcccga ccccggcagc
     1681 gccgacgaga agtacggtgc ggccgatccg gatatcttgc ggacgtggct gacgcgaagt
     1741 ggcgcttcga gcgtctaatc ggttgcgatg ccctacctcc ggccatcctt cgcggctttc
     1801 gctccacttt ttctattgac cggattcctc gctcttccta tcggcactcc gtctcaggag
     1861 aaagaaaaag agcaggtgaa aggaacgccg gcggagcctg ccgacgcggc cgatgtccgc
     1921 gagcaaattg cgatcgtcga gaagttgaag tcgatcgttc ctgaccgagg cgcggtcctc
     1981 tattttcttt ccacggcgaa gcagcacctg ggagaaaccc gcgaagccct ggctctctta
     2041 aaggaatgtc tagctttgcg tgagggtttc gacccgtccg gggaagcggc gtttcttgga
     2101 ctcaagggca caaaagaatt tgatgacctt gtcgcgtcgg tgcaccgcga ttttccggcc
     2161 gcagcgcatg cccggctcgc ttttgtcacg gatgagaaag acctcattcc tgagggactg
     2221 acgcacgacg cgaagcaaaa tcttttttat ttgagcagtc agtatcgaag aaagatcgtg
     2281 aaaatcgatc cgcaaggtcg ggtttctgat tttgtgcccg gggatcgcta cggacttctg
     2341 cctgtgcttg gcatccgttt ggatgccagt gacggcacgg tctgggcgga cacatttgaa
     2401 gacgcgggcc ggacggagat cgtgcatttc gatg
//
LOCUS       DASYVO010000462         4072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_67514, whole genome shotgun sequence.
ACCESSION   DASYVO010000462 DASYVO010000000
VERSION     DASYVO010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67514"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VGR03_15785"
     CDS             complement(<1..490)
                     /locus_tag="VGR03_15785"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV1995793.1"
                     /translation="MSNTTQAAQAGPIILEGRHDGIATLVMNRPDRLNALNNELATAL
                     NEALGRIAEDDTVQVVVITGAGRAFCAGGDLAVIGKGRRSRETQELEPLLRAGMQAVL
                     KMRTMPQPVIAAVNGAAAGAGMNIALAADIRIAAEEATFGQNFAKVGLFPDYGGTYFL
                     PQL"
     gene            complement(503..1033)
                     /locus_tag="VGR03_15790"
     CDS             complement(503..1033)
                     /locus_tag="VGR03_15790"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HEV1995794.1"
                     /translation="MRTLPDHLRKGMKLVIVGCNPSESSVRVGHYYAGRGNEFWPMLH
                     ESGVVPEPFDYHDDKRVIEFGVGLTDLVKRPTKGIEELKREDYAEGRIVLSQKLEEFA
                     PHVVAFNGKLTYEQFSQRKCKFGMQKETLYGARVYVLPSTSGANATGRSEKLRHFRKL
                     AQLVARIGKEHEARSH"
     gene            1572..2225
                     /locus_tag="VGR03_15795"
     CDS             1572..2225
                     /locus_tag="VGR03_15795"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1995795.1"
                     /translation="MRPPIGLPKPLSAPNPHRLPSTVARDDEHLLVAAAKRGDAHAFE
                     ELVNRYEAKIFRLTMNITRNREDAEDAMQDAFLKSYAHLKDFQEDSRFYTWLVRIAAN
                     EALMKLRKRRPNQFSLDEPVEGDEDLMPRELEDWGPSPEQRFAQTEMHEILSEVIDNL
                     EPDFRVVFTLRDVEELSTEETAKVLGISVPAVKSRLLRARLKLRQKLNRYFRQGEPN"
     gene            2222..2461
                     /locus_tag="VGR03_15800"
     CDS             2222..2461
                     /locus_tag="VGR03_15800"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEV1995796.1"
                     /translation="MNCKGVIREISNYIDGDLGLSAKQELERHLEHCEDCKMVVDQTR
                     LTVDVFCGSKPVELSGGVKSRLHDALHRKLHEKGN"
     gene            complement(2471..3448)
                     /locus_tag="VGR03_15805"
     CDS             complement(2471..3448)
                     /locus_tag="VGR03_15805"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEV1995797.1"
                     /translation="MTLHISEGEVRAVLTMPLAVEAVEEISRKQATGEVVVHPRRRFE
                     LPGGGFFHYMAAADFSLGFVAMKQYTYVRGKLRFLVPLYEMATGDLLALIEADYMGQL
                     RTGAASGVATKYLARKDARVAAIIGTGGQAKTQLEAIAAVRKLESARAYGRDAAKREK
                     FCEEMSERLGIPVQPSPSAAEAVRGADIVSTATTTSQPVVCGADLAPGAHINAIGANH
                     AHKRELDDEAVASADVIVVDSVEQSRQEAGDLIIAFHGDEICWTGVKKLSEIVAGKAS
                     GRTSDTEVTLFKSNGIASWDLAVAMKVYAMAREKGLGRELPLWSDGAKS"
     gene            complement(3560..>4072)
                     /locus_tag="VGR03_15810"
     CDS             complement(3560..>4072)
                     /locus_tag="VGR03_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995798.1"
                     /translation="ASARAAETTNTTQPGDQTNPIAGWVIARFIELDLPDPVREGAAS
                     ANVRPLAWFELNRVNDPSGDKPQYLVAAGRGPEGQVCDFTALRVYTWYVKKSRYETAF
                     IENNLCGQLPVRLSKGPKAEPEFRFRVMDGNKEEHVYRLNQTVVRRVREASEAPGKHD
                     SAKQAKPAAK"
BASE COUNT          788 a         1299 c         1112 g          873 t
ORIGIN      
        1 ccaactgcgg caggaaatac gtgccgccat aatccggaaa gaggcctacc ttggcgaaat
       61 tctgcccgaa ggtcgcttct tccgcggcaa ttcgaatatc cgccgccagc gcaatgttca
      121 tgcccgcacc ggccgcagcg ccgttcacgg cggcgatcac tggctggggc atggtgcgca
      181 tcttcaggac cgcttgcatt cccgcacgca agagcggctc cagttcttgc gtttcacggc
      241 tccggcggcc ttttccgatc accgcgaggt ccccgccggc gcagaaagcg cggcccgctc
      301 cggtgatcac caccacttgg acggtgtcat cctccgcgat ccgccccagc gcttcattga
      361 gcgccgtggc cagttcgttg ttcagcgcat tcaaccggtc ggggcgattc atcaccaatg
      421 tggcgatgcc atcgtgcctc ccttccagga tgattggtcc cgcttgcgcg gcttgtgtag
      481 tgttgctcac ggggaactcc gttcagtggc ttcgggcctc gtgttcctta ccgatgcgag
      541 caacgagttg cgccagcttt ctaaaatgcc gcagcttctc gctcctgccc gtggcattcg
      601 cgccgctggt ggaaggcagg acgtacacac gcgcgccata gagcgtctct ttctgcatgc
      661 caaacttgca ttttcgctga gaaaattgct cgtaagtaag tttcccgtta aaggccacaa
      721 cgtgaggagc gaattcctcg agcttttgcg agaggacgat gcggccttcg gcgtagtctt
      781 cgcgcttcag ttcctcaatg cctttcgtgg ggcgcttcac caggtcggta agcccaacgc
      841 cgaattcgat cactcgcttg tcgtcgtggt aatcgaaagg ctccggcaca acgccgctct
      901 cgtggagcat gggccagaat tcgttcccgc ggccggcgta gtagtggcca acgcggacgg
      961 aggactcgct cggattgcag cccacgatca cgagcttcat gcctttgcgg aggtggtcgg
     1021 gaagtgttcg cattacgcca gcttactatg ccttttgatt ttactactta cccttcaatc
     1081 ggggagcgca cttttcgata tgggcggccg ggccttcctt ggcatcctcg ctcgtaaaga
     1141 gcaacagctc gggttccccc tcgatgctat tcactgattc tgttcgtcgc gccggagagg
     1201 cacaggacgc cgaaccgagg cttgagcgcc gaaacagacc gccggaaccg cgttcgtcga
     1261 atggctacca cgccaaattg tactagcggc gccgccaagc ggcaacttcc gcccgaccag
     1321 gttcttcaag tctgtcccta cgatggaagt cccccagtgt caggatgcgc catgaagcac
     1381 tctaagtagc tcacaatgaa taacttgagc atggctttgc agcggcaacc cataccctgc
     1441 cattctcacc gcgcagcaat cccgtaactt tcccccaatt cctgaatctc atcgccagca
     1501 gcatcatcaa aataggagga cggcggaagt gttccatgct ctgtgctacc tttatactcc
     1561 cgaaagcgag gatgcggccg cccatcggcc tgcctaaacc attgagcgca ccgaatcccc
     1621 accgtctccc ttccaccgtc gcgcgcgacg acgaacacct gctggtggcc gccgccaagc
     1681 gcggcgatgc gcacgccttc gaagagctgg taaaccgcta tgaagcgaaa atcttccggc
     1741 tgaccatgaa catcacgcgg aatcgcgaag acgccgagga cgccatgcaa gacgccttcc
     1801 tcaagtctta tgcacacctc aaggatttcc aggaagactc tcgcttttac acctggctgg
     1861 tgcgtatcgc cgcgaacgaa gcgctgatga agttgcgcaa acgccgccca aaccagtttt
     1921 cgctcgacga gccggtggaa ggcgatgaag acctcatgcc ccgcgagctt gaggattggg
     1981 gaccaagtcc ggaacagcgg tttgcccaga cggaaatgca cgaaatcctt tccgaggtca
     2041 tcgacaatct cgagccggat ttccgggttg tttttacgct gcgcgacgtc gaagagcttt
     2101 cgaccgaaga aaccgccaaa gtcctcggca tttcggttcc cgcggtaaag tcccggctgc
     2161 tgcgagcccg gctcaaactc cgtcagaagc tgaatcgtta ttttcggcag ggtgaaccaa
     2221 attgaattgc aaaggcgtta ttcgcgagat ttccaactat atcgacggcg atctcggttt
     2281 atctgcgaag caggaactcg agcgccatct cgagcactgt gaagactgca agatggtggt
     2341 cgatcaaacc aggctgactg tggatgtctt ttgcggctcg aaaccggtgg aattgtccgg
     2401 cggagtgaag tcacggctgc atgatgcctt gcaccgcaag ctccatgaaa aaggcaatta
     2461 ggctgttctc ctagcttttt gctccatcac tccatagtgg caattccctg ccaagtcctt
     2521 tctctcgcgc catcgcgtag accttcatcg ccacggcgag atcccaggac gcaatcccgt
     2581 tcgacttgaa cagcgtgact tccgtatcgc tggttctgcc gctggctttc cccgcgacaa
     2641 tttcggaaag ctttttgacc ccggtccagc aaatctcatc gccatgaaaa gcgatgatca
     2701 gatcgcccgc ttcctgccgc gactgctcga cggaatccac gacaatgacg tcggcgctgg
     2761 cgactgcttc gtcgtccagc tcgcgcttgt gcgcgtggtt ggcgccaatg gcattgatgt
     2821 gggccccggg cgccagatcc gctccacata caaccggctg cgaggtagta gtggctgtcg
     2881 agacgatatc cgcgccgcgc accgcctctg ccgcggatgg actcggctgc acgggaatgc
     2941 ccagccgttc ggacatctct tcgcagaact tctcgcgctt cgccgcatcg cgcccatagg
     3001 cgcgcgcgga ttccagcttt cgtaccgcgg cgattgcttc aagctgcgtc ttcgcctgcc
     3061 ccccggtgcc aatgattgcc gcgacgcgag catccttacg cgccaggtat ttcgtggcca
     3121 cgccagaagc cgcgccggtc ctcaattgcc ccatgtaatc cgcttcgatc agcgccagca
     3181 agtcgcccgt ggccatctcg tagagaggaa caagaaaccg tagcttgccg cgcacatacg
     3241 tgtactgctt catggcgaca aagcccaggg agaaatccgc cgcggccata tagtgaaaga
     3301 atccgccacc gggcaattcg aatcgcctgc gcgggtgcac caccacttcc cccgtggcct
     3361 gcttgcgcga aatctcttcc acggcttcca ccgccagtgg catcgtcaac accgcccgca
     3421 cttccccttc gctgatatgc agcgtcatcg tttcactctc cgccttagcg tcttgatctc
     3481 cggatgcgcg cgacatcttt ggtatccaca atccatccga ccgcactgtc caaatcggca
     3541 agattccgga ccacatccgt catttcgccg ctggctttgc ctgcttcgcc gagtcgtgtt
     3601 ttcccggagc ttcgctcgct tcacggacgc ggcgaacaac tgtctggttc aagcggtaga
     3661 cgtgttcctc tttgttcccg tccatcaccc ggaagcgaaa ctcgggttcg gccttgggcc
     3721 ctttgctgag gcgaacaggt aattggccgc acaggttatt ttcgatgaat gcggtctcgt
     3781 acctgctctt cttgacgtac caggtgtaaa cgcggagcgc cgtaaagtcg cagacttggc
     3841 cctccggccc gcgccccgcg gccaccagat actgtggctt gtcgcccgac gggtcgttca
     3901 cgcgattcag ttcaaaccat gccaacgggc gaacatttgc agaagccgcc ccctcgcgca
     3961 cgggatcggg cagatccagc tcgatgaagc gggcaatgac ccagccggca atgggattgg
     4021 tttgatcgcc gggttgcgtt gtgttcgtgg tctcggctgc gcgggctgag gc
//
LOCUS       DASYVO010000463         8236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_7722, whole genome shotgun sequence.
ACCESSION   DASYVO010000463 DASYVO010000000
VERSION     DASYVO010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8236
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7722"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VGR03_15815"
     CDS             <1..184
                     /locus_tag="VGR03_15815"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HEV1995799.1"
                     /translation="EGSTLRQDNSANRKIYGRDLSARNIVREQEVGVPAAGEHLVSLL
                     NQRSPRNHSEPKSLTE"
     gene            529..921
                     /locus_tag="VGR03_15820"
     CDS             529..921
                     /locus_tag="VGR03_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995800.1"
                     /translation="MISLPLSAQKIELFKQAGRGVPGEKGLEKIALAVLEDFDWKEVT
                     VMDLSGTHEYFRIGAKGGHQQVGIHVHLPYGQFDPAKGYDAGALKAGSDAVGLVGDNR
                     RPGTVNNYVFELLKALEQGRYRAGLPKG"
     gene            complement(982..2523)
                     /locus_tag="VGR03_15825"
     CDS             complement(982..2523)
                     /locus_tag="VGR03_15825"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEV1995801.1"
                     /translation="MKTSYIIRSFATSWVAGLASAIVGFFLTPYVLHHLGDTEFGLWT
                     LVTTLTGYYGFLDLGVRSAILRFVSRSRALDNQDEINRVICTAFYFYLSVCLVAFAVT
                     GLVFPWLPQTFGVHGETAHAFGRLFLLMGVLQGISLPLNVFAGSVNAAGRFDLAYVVE
                     TLGLGVRVVLVITAVRAGGGLFAVGAAMLFANLFCDLLQIPLALWLIRGLSLRPKWVS
                     KASLREMFRYASVTFGVGVGDMLKANISPVLVGLFMNPAAVTFFSLPARLLRFPVDGT
                     AVMTGIVNPASSHLEAREDFTSLRRLIRLSVQSAFLILAPMSAILFIYGRELLRLWVG
                     EAYVSAFPLLVLLTLGLGCGATQACVQSMLFGIGRHKGLIAYRLGEAGAITLIGGVML
                     KMYGLEAFALVIAITLMITSLVLVPRHLCRMLGLPLGSYLKDGFLKPVLLTLPFAATL
                     YALHSWTVVRSWFGLVGVLAAGGVVHLATLAWAAFRPGKKSENQWTSLGVLEIVAERL
                     RPILL"
     gene            2994..4478
                     /locus_tag="VGR03_15830"
     CDS             2994..4478
                     /locus_tag="VGR03_15830"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEV1995802.1"
                     /translation="MNKFDSIRQPEQLSDAKRLLLQLYSRGSKQDQPASVPAISRRAQ
                     GSPPPLGLAQEQIWRHAQRLVDMPLLYNESITIYRTGPLDVDALERSFTEIVRRHDIW
                     RTTYDTLDGQPVQIIHPAPDRISLPVADLRGLPVVGREKEALRLATAEARRPFNMREG
                     PLVRPTLVRLGDEEYRLFLIMHQSIVDGVSAYEVLPSELAALYNAFSAGKPSPLPELP
                     VQYADFASWQREWLQGEELTRQMAYWRNQLAGELPVLQWPNDRPRPASQSYRGAILPF
                     GFSRELTASLRRWSQQEEVTLFMTLLAGFTALLHHYTQQADIVVGTPAPSGRKRSEVQ
                     ALMGYFLNPLPLRMSVAGNPTFRELLCQAREVTSGAISNDDVPLEYLAKELCLKQDAS
                     RHPFFQVAISLAPEASISNSGWDMTAMDVDSGGARWDLYLVLSERPSGMLGRAQYNPD
                     LFEENTISSTIEHFQRLLEVLGSNPALRVSESAFPEEGHSRSRA"
     gene            4504..7782
                     /locus_tag="VGR03_15835"
     CDS             4504..7782
                     /locus_tag="VGR03_15835"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEV1995803.1"
                     /translation="MSGDKDETLSAARLSENKRRLLEGFLRGEFGKSAAERPALTRRP
                     PNEPIPLSFPQVQVWLHAQMAAEIPFYNETMTIYRRGPLAPAVLERCLLEIIRRHEIW
                     RTTFEVREGTPVQVVHAPPSSILFPSTDLRNLPEPEREAEARRLATRDARIPFDLKER
                     PLLRSHLVRTGDEEYRLYITLHQIIVDAVTAYSVFLPELAALYEAFSAGKPSPLADLA
                     YQYGDFAFWQRNTLAPGAWSKHMAYWRKQLSGQLPVLEWPAARPRPAVETHRGEIQHF
                     RLEKELIGPLRAMSQREGASLSMTMLAGLAALLHRYTGQDDLILGSLSAGRKLTETES
                     LFGYFVNPFPLRIDLSGKPTFREMIARVRAVVLDGLSDEEVPFIKVVEEIQQKPDPSR
                     NPIFQITLSQQPQVSRPPAGWDLIIEEISNGGSKMDLLVVLDDRQDTIFVTITYNLDL
                     FDSSTIAGMMGHLQTLLGAAAVEPGKPIDELPILTPAEREQILIAWNDTAADYPTNRC
                     LHELIEAQAGKTPDATAVIFEQQKVSYRELNARANRLAHHLEKVGVGPEVLVGVCMER
                     SVDLVVALLGVLKAGGAYVPLDPEYPKDRLRMMLDDSRPPLLLTQQHLLDRLPAISAQ
                     IICLDADWHRIAGESDANPAVGTNPENLAYAIYTSGSTGQPKGVLNTHAGIVNRLLWM
                     QDAYRLTPSDRVLQKTPSSFDVSVWEFFWPLMTGACLVVARPDGHRDAGYLIDLIRRE
                     RITTLHFVPSMLQVFLEVEGVESCDSLKRVICSGEALPLKVQERFFARSKAELHNLYG
                     PTEAAVDVTSWQCRPDSSLFTVPIGRPIANVRIYLLDRNLQPVPVGVPGELYIAGVAL
                     ARGYLNRPELTAERFIPDPFRNQPGARLYKTGDLARYRSDGSIEYLRRVDDQVKIRGF
                     RIELGEIESVLRMHPAVRDARVIVREDVPDDRRLVAYLVLAEAAASLTEIGNYLKEKL
                     PAYMIPELVSLGQLPLTTSGKLDRRALPAPVHERLQQEEGTGEPEDEIEKLLAELWKD
                     ALKLDRVTIYDNFLDLGGHSLLVILMVARLQSQLGVRVKPNEVAFQTLGQLAAVCRER
                     LRRP"
     gene            7779..>8236
                     /locus_tag="VGR03_15840"
     CDS             7779..>8236
                     /locus_tag="VGR03_15840"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1995804.1"
                     /translation="MMASAEGILPLYFGLPETLLYGCFHEPHAGRSRDCAVVLCPPIG
                     HEYVNSHRALRQLAARLSDAGFPVLRFDYFGSGDSSGDIEEGSIPQWLDDISQAILEI
                     RRRTGLTSLCLAGLRLGASLSLLAAAERSDIASLVLWDPVVKGRSYLNGLL"
BASE COUNT         1746 a         2448 c         2441 g         1601 t
ORIGIN      
        1 ggagggctcc acgctccgtc aagacaacag cgcgaatcga aagatctacg gccgggatct
       61 gagcgcgcgg aacatcgtgc gcgagcagga agtaggcgtg cccgcagcag gagagcacct
      121 ggtctcgtta ttgaaccagc gctcgccgcg caatcattcg gagccgaaat cactgactga
      181 gtaacggcga tcgccaccgc gcgttcgctt gacagtcgtg acgcgggcat ccggcggcag
      241 cggcgctgtc cgcgaagtcg tggaattaat cctaaagtcc cagggaaaat ggtcggtgct
      301 gatcgggcgc gcccgcgcct gaagatcggt tgcgccgagg gaaaaaacaa tcgaaagttg
      361 aagggatcga gggaacgcgt gaagctgcaa gtttgcgcga cggaagcaca gcgctgcttc
      421 gagggcttgc ttccctccga tatgcctata aaatccctta tcaaacatcc agtattcttt
      481 gggtgtcgag gcatcgagac tcttgccgat tgccgggagg tttgagccat gatcagcctg
      541 cccttaagcg cccagaagat cgaactcttc aagcaagcag gccgcggtgt acccggagaa
      601 aaaggactgg agaagatcgc cctcgccgta ctcgaggact tcgattggaa agaagtcacc
      661 gtgatggatc tttccggcac ccacgagtat tttcggatcg gggccaaggg aggtcaccag
      721 caggttggga ttcacgtcca cttgccctac ggccagttcg accccgccaa ggggtatgac
      781 gcgggggcgt tgaaggcagg aagcgatgcg gtcggtctcg tcggagataa ccgtaggccg
      841 ggaacggtga acaattatgt ttttgagctt ctaaaagcac tggagcaggg ccgctaccgc
      901 gccggcctgc cgaagggtta gcctccatct cggtagtgcg agaatcgcct ccagaaactt
      961 ccgcgctttc ggtccataga gttacagaag gatggggcga agcctttccg caacgatctc
     1021 cagaacgccc agcgacgtcc actggttctc ggatttcttt cccggccgga aggccgccca
     1081 cgccagcgtg gccaggtgca ccacgccgcc cgcggcgagc acaccgacca gcccgaacca
     1141 ggacctgacc acggtccaag agtgcaaagc gtacaatgtc gcggcaaacg ggagggtcaa
     1201 aagaacgggt tttaagaagc catccttcaa ataggagccg agagggagtc ccaacattcg
     1261 gcacaggtgc cgcggaacga gaaccaggct ggtgatcatc aacgtaatcg caatcaccag
     1321 ggcgaaggcc tccagtccgt acatcttcaa catgacgccg ccgatcaagg tgatcgctcc
     1381 ggcctctcca agccggtatg cgatcaatcc tttgtgccgg ccgatgccga acaacatgga
     1441 ctgaacacac gcctgtgtgg ccccgcaacc taatcccagc gtcagcagca ctagaagcgg
     1501 gaaggccgaa acgtaggcct cgcccaccca caaccggagc agttcgcgac cgtagatgaa
     1561 aagaatggca gacatgggag ccaaaatcag aaaagcgctc tgcacgctca gccggatcag
     1621 ccggcggagg ctggtgaagt cctcgcgggc ctccaggtgg ctcgaagcgg ggttcacgat
     1681 tccggtcatt accgctgtcc cgtcgactgg aaaccgcagg agccttgccg gcagagaaaa
     1741 gaaagtcacc gccgcggggt tcatgaacag acccaccagc accggggaaa tgttggcctt
     1801 caacatgtct ccgacgccga cgccaaaggt caccgaggcg taacgaaaca tctcccgcag
     1861 cgaagctttg ctcacccact tcggacggag gctcagtccc cggatcaacc acagtgccag
     1921 cggaatctgc aggaggtcgc agaagaggtt cgcgaacagc atcgccgctc ccaccgcgaa
     1981 caaaccgcct ccggcgcgaa ccgcggtgat caccagcacg acgcgcacgc ccaggccgag
     2041 agtctccaca acgtaagcga ggtcgaaccg gccggcagcg ttcaccgacc cggcaaaaac
     2101 attcaaggga agcgagattc cctgcaagac tcccataagg agaaagagac gcccaaaggc
     2161 atgcgccgtt tccccatgga ctccaaaagt ttgtggcagc caggggaaca ccagtccggt
     2221 caccgcgaat gctacaagac aaacgctgag atagaaatag aacgcggtgc aaatcacgcg
     2281 gttgatctcg tcctgattgt cgagagcccg ggaccgggag acgaaacgaa gaatggccga
     2341 gcgaaccccg agatcgagaa acccatagta gcccgtcagc gtggtcacca gagtccacag
     2401 gccgaactcc gtgtctccca ggtggtgcag cacgtagggc gtcagaaaaa atccaacgat
     2461 ggcgctggcc agccctgcca cccaactggt ggcaaaagac cggatgatgt aagaggtctt
     2521 catgcgctat tcattgccca cggttccgca tcggaggatc gaagccccgc cgggagtgcc
     2581 aggggcggct cggatttcaa attctgaggg agagctacaa tgtcttcgaa cgccatccca
     2641 gaagtgagga acagtagcaa cgaggtggga agcagatgac aggggaatag agagttgttt
     2701 tcctcattct gcaagggcgc gctcaaccat cgctgataac ctgctcgttc ctctgccctc
     2761 caaggcactt tgaggagtgg gcagaggttt tccgcaagat agaaagcatt tgcattctcc
     2821 aacgaatacc atagaaaatc aagatgactg accgccaagc gtatgctatc tctctccaac
     2881 attaggtctt actggcgcaa cacgcatgga gaatcgcccg accttggcca tgggattgcc
     2941 ttccgtgcat tccgcctccc caccaggcag gcgggatcgc gcgggcaaac gatatgaaca
     3001 agtttgattc tatccgccag ccggagcagc tctcagacgc caagcgcctc ctgcttcagc
     3061 tctattcgcg aggcagcaag caagaccagc cggcgagcgt ccccgctatc tcgcggcgcg
     3121 cgcaaggcag cccgccaccc ttgggattgg cgcaggaaca gatttggcgt catgcccaaa
     3181 gacttgtaga catgcctctg ctctacaacg aatccatcac catctaccgg actggccctc
     3241 tcgacgtgga cgcgctggag cgtagcttca cagagatcgt tcggcgtcac gacatctggc
     3301 gaacaaccta tgacacgctc gacgggcaac ccgtccagat tatccaccct gcgccggaca
     3361 ggatttcact tcccgtggcg gatctacgag gtcttcccgt ggttgggcgg gagaaggaag
     3421 cactgcggct ggccacggca gaggcccgcc ggccgttcaa tatgcgggaa ggtccgttgg
     3481 tgcggccaac gctggtgcgt ctcggggacg aggaataccg gttgtttttg atcatgcacc
     3541 aaagcatcgt ggacggggtt tccgcgtacg aggtgctccc ctcggagttg gccgcgctct
     3601 acaatgcctt ctcggctggc aagccctctc cgctgccgga gctgcccgta cagtacgccg
     3661 acttcgcctc ctggcagcga gaatggctgc aaggggaaga gctgaccagg cagatggcct
     3721 attggcggaa ccagctcgct ggggaactcc cggttttgca gtggcccaac gaccgcccac
     3781 gaccagcctc gcaatcgtat cgaggtgcga tccttccgtt tggcttttcg cgggaactga
     3841 ccgcgtcgct caggcgatgg agccagcaag aggaagtcac actgtttatg accttgctgg
     3901 ccggttttac tgccttgctg catcactaca cgcagcaggc tgacattgtt gtggggacgc
     3961 ctgctccgtc tggccgcaag cggtccgaag tgcaagcgct gatgggttat tttctgaacc
     4021 cgttgcccct ccggatgagt gtggcgggca atcccacttt tcgcgaactg ctttgtcagg
     4081 cgcgtgaagt tacttccggg gccatctcca acgatgacgt tcccttggag tatctggcga
     4141 aggaattgtg tctcaagcag gatgccagcc gtcatccctt ttttcaggtc gccattagcc
     4201 ttgcgcccga ggcctccata tcgaattccg ggtgggacat gactgcgatg gatgtagaca
     4261 gcggcggagc gagatgggat ttgtatcttg tgttgagcga aaggccttcc ggcatgctcg
     4321 gccgcgctca gtacaatccc gacctgtttg aagagaatac catctcctcg acgatcgagc
     4381 attttcagag attgcttgaa gtgctgggct cgaatccggc gctacgcgtc tcggaatcgg
     4441 catttcccga ggaaggccat tcgcgaagcc gggcctgatg gaagatgaaa gacgccgtga
     4501 caaatgagcg gggataagga tgagacgctg agcgccgcgc gcctgtcgga gaacaagcgc
     4561 aggctcctcg aagggtttct tcgcggcgaa ttcggcaagt ccgcggcgga gcgtcccgct
     4621 ctcacgcgcc gtcctccgaa tgagcccatc cctctctcct ttccgcaagt tcaggtgtgg
     4681 ctgcacgcgc agatggccgc cgagattccc ttctacaacg agaccatgac catctatcgc
     4741 cgcggcccgc tcgcaccggc ggtgctggaa cggtgcctgc tcgaaattat ccgccgccac
     4801 gagatttggc ggacgacgtt tgaggtgcgc gaaggcactc ccgtccaggt tgtccacgcg
     4861 ccgccatcca gcatcctatt tccgtccacc gacctgcgca atctgccgga acccgaacgc
     4921 gaggccgagg ccaggcgtct ggctaccagg gatgcgcgaa tccccttcga cctgaaagaa
     4981 aggccgctgt tgcgctctca cctggtgcgc acgggcgatg aggaatatcg cctgtacata
     5041 acccttcacc aaatcatcgt cgacgcggta actgcttaca gcgtgttttt gccggaactc
     5101 gcggccttat acgaggcctt ttctgcgggc aagccgtcac cactggccga tcttgcctat
     5161 cagtatggag actttgcctt ctggcagcgg aacacgctgg caccaggggc ttggtcgaaa
     5221 cacatggctt actggcggaa gcaactctcc ggccagctcc ccgtcctgga gtggcccgcc
     5281 gcccgccccc ggcccgcagt ggaaactcac aggggagaga tccagcattt tagattggag
     5341 aaggagttga ttggcccgct gcgggcgatg agccagcgcg aaggcgcttc tctctccatg
     5401 accatgctcg ctggtttggc ggcattgctc caccgctata cgggccagga tgacctgatc
     5461 ctgggaagcc tgtcggctgg ccgcaagttg acggaaacag aatcgctgtt cggttatttc
     5521 gtgaacccct tcccgttgcg catcgatctt tcgggcaagc cgacattccg ggaaatgatc
     5581 gcgcgcgtgc gggcggtagt tctggacggc ctgtctgatg aagaggtccc tttcattaag
     5641 gtcgtagaag aaatccagca gaaacccgat cccagccgaa acccaatctt tcagatcacg
     5701 ctttctcagc aaccgcaggt gtccaggccg cctgcggggt gggacctgat cattgaggaa
     5761 atatccaatg gcggctcaaa gatggacctg ctggtggtgc tcgacgaccg ccaggacacc
     5821 attttcgtta cgattacata taatctcgat cttttcgact cttcgaccat tgctgggatg
     5881 atggggcact tgcaaaccct gcttggcgcc gctgctgttg aaccggggaa gcccatcgac
     5941 gaattgccca tcctcacccc agcggagcgt gagcagattc tgatcgcatg gaacgatacc
     6001 gctgccgact accccacgaa ccggtgtttg cacgaactca tcgaagctca ggccggcaaa
     6061 acaccagacg caactgccgt aatattcgaa cagcaaaaag tgagttatcg agagttgaat
     6121 gcgcgcgcca accggctcgc gcatcatctg gaaaaggtag gggtaggtcc cgaggtgctg
     6181 gtcggcgtgt gtatggagcg ctccgtcgat ctggtggtag cgctgctggg cgtcctgaaa
     6241 gccggcggcg cctacgttcc gctcgacccc gagtatccca aggatcgcct gcggatgatg
     6301 ttggacgaca gccggcctcc gctgctgctt acacagcagc atcttctcga tcggcttcct
     6361 gcaatttcag cgcagatcat ttgtctggat gcagactggc atcgcatcgc gggggagagc
     6421 gatgcaaatc cagccgtggg aacgaatcca gaaaacctgg cttacgcgat ctatacatcc
     6481 ggttcgacgg gccagcccaa aggggtgttg aatacgcatg ccggcatcgt gaaccgtctg
     6541 ctctggatgc aggatgccta tcggttgacg ccctccgatc gcgttctgca aaagacgcct
     6601 tccagtttcg atgtttcagt ctgggagttt ttctggccgt tgatgaccgg cgcctgtctc
     6661 gtcgtggcca ggcccgacgg tcaccgggac gccggctacc tgattgatct aattcgccgc
     6721 gagcggatca ccacgctcca tttcgtgcct tcgatgcttc aggtattcct ggaggtcgag
     6781 ggtgttgaaa gttgcgacag cttgaagcgc gtgatctgta gcggtgaggc cctgcctttg
     6841 aaagtccagg agcgcttctt cgctcgctcc aaagcagaat tgcataactt gtatgggccc
     6901 accgaagcgg cggtggatgt gacgtcctgg cagtgtcgtc ccgacagcag tctcttcact
     6961 gtccccatcg gtcgtccgat cgccaacgtc cgaatctatt tgctcgatcg gaatctgcag
     7021 cccgtgccgg tgggcgtgcc cggagagctt tatattgcag gtgtggccct ggcgcggggc
     7081 tacctgaatc gccccgagct caccgcagag cgattcattc ccgatccctt ccgcaaccaa
     7141 ccgggtgcgc ggctttacaa gacgggcgac ctggcgcgtt atcgttcgga tggaagtatc
     7201 gaatacctgc gccgcgtgga tgaccaggtg aagatccgcg gctttcgaat cgaattgggc
     7261 gagatcgaat ccgtgctgcg catgcacccc gccgtccgcg acgctcgtgt tattgttcgc
     7321 gaagatgttc cggacgaccg gcgcctggtg gcctaccttg tgctggcgga ggcggccgcc
     7381 tcgctcacgg agattggcaa ttatttgaag gagaaattgc ccgcctacat gattcctgaa
     7441 ctggtcagcc tgggccagct gcccttgacc acgagcggaa agctggatcg gcgcgcgctt
     7501 cctgccccgg tccatgagag acttcaacag gaagaaggca ccggggaacc tgaggatgag
     7561 attgagaagc tgctcgccga actctggaag gacgccctaa agctggatcg cgtgaccatt
     7621 tacgacaact tcctggacct cggaggtcat tcgctcctgg tcatcctgat ggtggctcgc
     7681 ctgcaaagcc agttgggcgt gcgcgtcaag ccgaacgaag tagcgttcca gaccctgggg
     7741 cagcttgctg cggtgtgcag ggagcgattg cgacgtccat gatggcttct gctgaaggaa
     7801 tacttcccct gtacttcggc ctgccggaga cgttgctcta cggttgtttc cacgaaccgc
     7861 atgcagggcg aagccgggac tgcgcggtgg ttctttgccc gcccatcggg catgaatacg
     7921 tcaattccca tcgcgcgctt cgccaattgg cggctcgctt gtccgacgcc gggttccctg
     7981 tgctgcgatt tgattacttc ggaagtggcg attcctcagg cgacatcgag gaggggagta
     8041 tcccccaatg gctcgacgat atctcgcagg ccattttgga aatcaggcgg aggacaggtc
     8101 tcaccagcct ctgtctcgca ggactccggc tcggtgccag tctatcgctc ttggccgccg
     8161 ctgaacgcag cgacatcgcc agcctggtgc tctgggatcc ggtcgtgaag ggaaggagtt
     8221 atctcaacgg cttact
//
LOCUS       DASYVO010000464         1659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_208406, whole genome shotgun sequence.
ACCESSION   DASYVO010000464 DASYVO010000000
VERSION     DASYVO010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208406"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VGR03_15845"
     CDS             <1..975
                     /locus_tag="VGR03_15845"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1995805.1"
                     /translation="QLLPAALVVGGLSEGAQLQAIRKGARLVVATPGRLEDYLDRRLF
                     HFNALRILILDEADRMLDMGFLPAIKRIASILPKERQTMCFSATMEGDMARIVKDYLR
                     SAVRLSFGSTSKPNENVRVQAFEVAMDRKQEMLQRLLTKEKGKCLVFARTKRGTERIA
                     ESLNRKGFSAAMIHGDRSQSQRTTALTGFQQGRFRVLVATDLASRGIHVQDIAHVINY
                     DLPEVAENFIHRVGRTGRNGGHGVASTLFVKEQRSELFQLERSLGIKIERMRADETAS
                     LPERQERLPVNLPAFQDRKPVNLPRVESALVPERFVLPGEVFQTQLEN"
     gene            1112..>1659
                     /gene="recR"
                     /locus_tag="VGR03_15850"
     CDS             1112..>1659
                     /gene="recR"
                     /locus_tag="VGR03_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEV1995806.1"
                     /translation="MPDFAAPVERLIDELKHLPGIGQKTAQRLAFYLLRAAPEDALAL
                     ADAIRDAKEKIRACSVCHNITDTDPCLYCVGPSRSKKTICVVEEAHNIMAIEKTRMYS
                     GMYHVLGGSLSPLSGRGPEQLHIKTLIERLKGAAVEEIIIATNPTAEGEATAVYLSKL
                     LKPLGVRVTRIGVGIPVGADLE"
BASE COUNT          345 a          474 c          515 g          325 t
ORIGIN      
        1 caactgctac ctgcggcgct ggtcgtcggc ggcctgtccg aaggcgcgca gctgcaggcc
       61 attcgcaagg gagcgcggct ggttgtggcg actccggggc ggctggaaga ttacctcgac
      121 cgcagactct tccactttaa cgcactgcgt attctcatcc tcgatgaagc ggaccgcatg
      181 ctggacatgg gatttcttcc ggcgatcaag cgaatcgcct cgatcctgcc aaaagagcgg
      241 cagacgatgt gcttctccgc gacgatggaa ggcgatatgg cgcgcatcgt gaaggattat
      301 cttcgcagcg ccgtgcgtct ttcgtttggc tcgacgtcga aacctaacga gaatgtgcgc
      361 gtgcaggcgt ttgaagtcgc catggaccgc aagcaagaaa tgctgcaacg cttgctgacc
      421 aaggaaaagg gaaagtgcct ggtgtttgcg cgaaccaagc gcgggaccga acggattgcc
      481 gagagcttga accgtaaggg attctcggcc gccatgattc atggcgaccg ctcgcaatcg
      541 caacgcacga cggcgctgac tgggtttcaa caggggcgct tccgcgtgct ggtggcgacc
      601 gacctggctt cgcgcgggat ccacgtgcag gacatcgcac acgtcatcaa ctacgatctg
      661 cctgaagtgg cggagaattt tatccaccgc gttgggcgca cgggacgtaa cggcggacac
      721 ggcgtggctt caacgctttt cgtgaaagag cagcggtccg aattgttcca attggagcgt
      781 tcgctgggca tcaagatcga gcggatgcgc gcggatgaaa ctgcttccct ccccgaaaga
      841 caggaacggc tgccggtcaa tttgccagct tttcaggatc gtaaacctgt caaccttcct
      901 cgcgtggagt ctgccctcgt gccagagaga tttgtgcttc ccggcgaggt tttccagacg
      961 cagctcgaaa actaagcaag tctaattacg atgtgaaacg cgggcccccc gatcggatcg
     1021 gggggcccgt ctgcgtttgc ggcgcttaca tcttttctct tgatgagcag gcggtttgcg
     1081 ctgatgcgcg cggctatgct acacatgctg catgcctgat tttgccgctc ccgtcgaacg
     1141 actcatcgat gagctgaaac acctccccgg aatcggccag aagaccgcgc aacgcctggc
     1201 attttatctg ttgcgggcgg cgcctgagga tgcgctggcg ctggcggacg ccattcgcga
     1261 cgccaaggaa aaaatccgcg cgtgctccgt ctgccacaac atcacggata cggatccgtg
     1321 cttgtactgc gtgggaccga gccgcagcaa gaagaccatt tgcgtggtcg aagaagcgca
     1381 caacatcatg gcgattgaaa aaactcggat gtacagcggg atgtaccacg tgctaggcgg
     1441 gtcgctctcg ccgctatcgg ggcgcggacc ggagcaactt catatcaaga cgctgatcga
     1501 gcggctaaag ggcgccgcgg tcgaagagat catcatcgcg acgaatccca cggccgaagg
     1561 cgaagccaca gcggtatacc tctcaaagtt gttgaagccg ctgggtgtgc gcgtcacgcg
     1621 aatcggcgtt gggatccccg tgggcgcgga cctggaata
//
LOCUS       DASYVO010000465         4568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_67844, whole genome shotgun sequence.
ACCESSION   DASYVO010000465 DASYVO010000000
VERSION     DASYVO010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67844"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1537)
                     /locus_tag="VGR03_15855"
     CDS             complement(143..1537)
                     /locus_tag="VGR03_15855"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV1995807.1"
                     /translation="MKSHGIRVPSWTAVAAVVLCYAGIAMAQEAATPANPAPTKDVLQ
                     GTPAPSPALRLTLQDALARARKNSTQFQLALTDAGLAREDKIQARDALLPGVNYNTQA
                     LYTQSNGPGNGVKFIANNGVHEYVSQGNAHEVIDLAGFSNYRRASAAATVARARAEIA
                     SRGLVVTVVQGYYAVAAAQQKLQSAQKAADEGDRFFKLTQDLEHGGEVAHSDVIKAEL
                     QMQERRRQLLEAQLGFLNSRLDLAVLIFPDFNDNYEVADDLHSAVPLPTLAEVQQQAG
                     RDNPDLRAALAAVREAGAGVTGARAGYLPSLSLDYFYGIDAAQFTTHSVINGQKLSNL
                     GSSASATLSIPIWNWGATQSRIKQAELRRSQAQRELSLAQRKLLAEIRSLYAEAETTL
                     NELGGLNRSAELAAESLRLITLRYRDGEATVLEVVDAQSTNTQANSAYQDGAVRYRVA
                     LANVQTLTGVLTTP"
     gene            complement(1619..3091)
                     /locus_tag="VGR03_15860"
     CDS             complement(1619..3091)
                     /locus_tag="VGR03_15860"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HEV1995808.1"
                     /translation="MLDSVRVRLTLWYTAVLAVVLIVLSLITYFIFWRSTLQRTDLNL
                     SELSEAFLTTLDAEVQDQSGPDSLKLAGQVAITEHRFRDHIFAIFDATGKIVVSSQDV
                     PPAAAATGSSAAGPLSSHSFQRFLDASSHSERFFGEVKGGEDGYRGFARHFTSAGKTH
                     ILIILQSLHPQQEMLEEVASTFLWVIPIAIVLASVGGYFLARKSLAPVVAMSRQAGRI
                     GAANLHERLAVQNDRDELGQLAQSFNSLLDRLSQSFERQRRFMADASHELRTPVAILR
                     GEAEVALSQQARSLEEYRESLGVLHQEAERLTHIVEDLFTLTRADAGQYPLQPRDFYL
                     DELVGECVHSARTLASAKRISLNFEEASESPIHADESLLRRMILNLLDNAIKYTSEGG
                     RVTVTCRRAGGEYVLSITDTGGGIPAEQQPRIFERFFRVDKARSRAENDGGGAGLGLS
                     ISRWIAEAHHGRLELTRSDSIGSTFTAYLPAAPSPSASSG"
     gene            complement(3084..3845)
                     /locus_tag="VGR03_15865"
     CDS             complement(3084..3845)
                     /locus_tag="VGR03_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1995809.1"
                     /translation="MRLLLVEDDARIARFVAKGLREQAYAVDVSNTGDDALYQAAINT
                     YDLVILDVMIPGRNGFEVCRELRKSGQRMPILMLTARDAVEDRITGLDHGADDYLTKP
                     FEFRELLARLRALLRRSGELRPAKIAVADLVLDTAAQIVSRAGRSVTLTAKEYALLEY
                     LARSAGRVVGRAEIAEHVWDETFDPFSNLIEVYVNRVRRKIDADSSKPLLHTRRGAGY
                     FFGPLVDPDAADAADESASPRPRAKATSSVRKNHA"
     gene            4065..>4568
                     /locus_tag="VGR03_15870"
     CDS             4065..>4568
                     /locus_tag="VGR03_15870"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEV1995810.1"
                     /translation="MRPFGCMELWAGNERAHRSLDLAGLQSDVIAVPSGGDKGGDLSA
                     VFSCSDNTARVVLADCVGHGYTASGVARHVHHLLHKFQDIRDTAGLLAALNDAFTLSN
                     EKLDGPLRLTTVVTGTFDGTSGEFNFAYAAHPRMFLWREREKRFLELGEGLEGYPLGY
                     ITGETYSQ"
BASE COUNT          974 a         1292 c         1410 g          892 t
ORIGIN      
        1 ggcgaggtct gtacggagac tacgggatcc ttttctttta ccgttttgcc acaaccgctc
       61 atgccggaga gcagcgcgcc aatggctgcg acggtaacaa gcgaaactcc tcgcgtttgc
      121 aggtgaatcg ttgtcgatag tttcatggag ttgtcagcac ccctgtcaga gtttgcacat
      181 tggccagtgc cacgcgatag cgcacggcac cgtcctgata ggcactattg gcctgggtat
      241 tggtggattg cgcgtcgact acctcgagca cggtggcttc gccatctctg taacgcagtg
      301 tgataagccg caagctctcg gctgctagct ccgcggagcg gttgaggcca ccaagttcgt
      361 tcagcgtggt ttccgcttcc gcgtagagag accggatctc ggcgaggagt ttgcgttgag
      421 cgagagaaag ctcacgctgt gcctgagagc ggcgcagttc ggcctgcttg atgcggctct
      481 gcgtggcgcc ccagttccag atgggaatgc tcagcgtggc tgaggcagaa gaaccgagat
      541 tcgaaagctt ttgaccgttg atgacagagt gtgtagtaaa ttgtgcggcg tcgattccgt
      601 agaaatagtc gagacttaaa gagggtaaat agccggcgcg ggcgccagtg acaccggcgc
      661 cggcttcgcg gaccgcggcg agtgccgcgc gaagatcggg attatcccgg ccagcctgct
      721 gctgaacttc cgcgagcgtg ggtaacggca ctgcggaatg gagatcgtca gcaacttcgt
      781 agttgtcgtt gaaatctggg aaaatcaaaa ctgctaaatc gagccgactg tttaggaacc
      841 ccagctgggc ttccagcaat tgccggcggc gctcctgcat ctgcaattct gccttgatga
      901 cgtccgaatg agccacttcc ccgccatgtt cgaggtcctg cgtaagtttg aagaagcggt
      961 cgccttcgtc ggccgccttt tgcgcggact ggagcttctg ctgcgcagcg gcaacggcgt
     1021 aatagccctg aacaacggtc acaaccagac cgcgagaagc gatttcagcc cgcgcgcggg
     1081 cgaccgtagc ggcagcggag gcacggcgat aattggaaaa gccggccaag tcgatgactt
     1141 cgtgtgcgtt gccctggctc acgtattcgt gaacgccgtt gttcgcaatg aacttcacgc
     1201 cgttacccgg cccattgctt tgcgtgtaga gagcctgcgt gttgtagttt acgccgggga
     1261 gcagtgcgtc gcgcgcctgg atcttgtcct cgcgggcaag ccctgcatcg gtgagtgcca
     1321 actgaaattg cgtgctattt tttctggcgc gcgccagggc atcctgcagc gtcaggcgga
     1381 gcgctggcga cggagcagga gttccttgca gcacatcttt tgtgggtgca gggttcgcgg
     1441 gagtcgccgc ctcttgcgcc atggcaattc ctgcgtagca caacacaacg gcagcaaccg
     1501 ccgtccacga cggcactcga atgccatgcg atttcatgaa tctcgactcc tgggccggcc
     1561 gaaaacacgt acccggcaag gaagaatcta acctaacgga gcgaagataa acataggatt
     1621 aaccgctaga cgcagagggg gatggtgccg caggaagata agcggtgaag gtgcttccga
     1681 tcgaatcaga gcgcgtcagc tccaaacgtc cgtgatgggc ctcggcaatc caacgggaaa
     1741 tggataggcc cagcccagcg ccgccgccgt cgttctccgc ccgggaacgg gctttgtcta
     1801 cacggaagaa gcgttcgaaa atgcgcggct gttgctccgc ggggatgcct cccccggtat
     1861 ccgtgatgct caagacatat tccccgccgg cgcggcggca ggtcaccgtg acgcgtccac
     1921 cctccgaggt gtacttaatc gcgttatcca gcagattgag gatcatgcgg cgcagcaggg
     1981 attcgtcggc gtggatagga gactcggatg cttcttcgaa attcagagaa atcctctttg
     2041 cggatgccag cgttctggcg gaatgcacgc actcgccaac gagttcatcg agataaaaat
     2101 cgcgcggttg gagcggatac tggccggcgt ccgcgcgcgt aagcgtgaag aggtcctcta
     2161 cgatatgcgt gagccgttcg gcctcctggt gaagcactcc cagcgattcc cggtattcct
     2221 ccagcgaacg tgcctgctgc gaaagagcga cctccgcttc gccgcgcaaa atcgcaaccg
     2281 gcgtgcgcag ctcgtgcgac gcatcggcca tgaagcgccg ctggcgctcg aaagattggc
     2341 tgaggcggtc gagcaggctg ttgaacgact gcgccagctg ccccagttcg tccctatcat
     2401 tctgtacggc caagcgctcg tgtagattgg cggccccgat gcgcccggcc tgcctgctca
     2461 tcgccacaac gggggccaga ctcttgcgcg cgagaaagta accgcccaca ctggcaagta
     2521 caatggcgat cgggatgacc cataaaaacg tcgaggcaac ttcttcgagc atctcctgct
     2581 ggggatggag cgattgtagg atgatcagga tgtgtgtttt ccctgctgac gtaaaatgtc
     2641 gcgcaaaacc ccggtagccg tcctcgcctc ccttgacttc gccgaaaaac cgttctgaat
     2701 gggaggaggc atcgagaaag cgttgaaacg aatgcgagga caaaggtccg gcggcagacg
     2761 agccagtcgc cgccgctgcg ggcggaacat cctgggagct gacaacgatt ttccccgtcg
     2821 catcaaagat cgcgaaaata tgatcgcgaa agcgatgttc cgtgatggcc acctgcccgg
     2881 ccagtttcag cgaatcgggg ccactttgat cttgcacttc agcatcaaga gtggtaagaa
     2941 atgcttcgga gagttcggag agattcaggt cggtgcgctg gagtgtgctc cgccagaaaa
     3001 taaaataagt gatcagggag agaacaatca gtactaccgc caatactgcc gtataccaga
     3061 gcgtgagtcg gacgcggacg gaatcaagca tggttcttcc ggaccgaaga agtcgccttc
     3121 gcacgaggcc gcggggaagc actttcgtct gccgcgtccg cggcatccgg atcgaccaga
     3181 gggccaaaga agtagccagc gccgcggcgg gtatggagca agggcttgct ggaatcggcg
     3241 tcaattttcc ggcgcacgcg gttcacgtaa acttcgatca gattcgagaa gggatcaaac
     3301 gtctcatccc aaacgtgttc ggcgatttcc gcgcgcccca caacgcggcc ggcgctgcgg
     3361 gcaagatatt cgagcagcgc gtattcttta gcggtgagcg tcacactccg ccccgcccgg
     3421 gaaacgattt gcgcggcagt atccaggacg aggtcggcga ccgcaatttt ggcggggcgg
     3481 agttcacccg agcggcgcag caaggcgcgg agacgcgcga gcaactcgcg gaattcgaac
     3541 ggcttggtga ggtaatcgtc agcaccgtga tcgaggccgg tgatgcggtc ctccacggca
     3601 tcgcgcgcgg tcagcatcag aatgggcatg cgctggcctg attttcgcag ctcacggcag
     3661 acttcaaaac cgttgcggcc cggaatcatc acatcgagga tcaccaggtc gtaagtattg
     3721 atcgctgcct gataaagcgc atcgtcgccg gtattggaaa catcaacggc atacgcttgt
     3781 tcgcgcaaac ccttggccac aaatcgcgcg atgcgcgcat cgtcttcgac aagcagtagc
     3841 cgcatgcgag catcatacgg caggcgcgag gaaaagtgag atacttgctg acgggacgtg
     3901 acgcaagtcc gccgttcctg gcgagttgat tcacgcaccc ggatcaagac gcaaggtgcc
     3961 ggttcatcca cgaagactgc acccaagaaa gtcccgcaag catcaaagct gctatgaagt
     4021 tcaagaaggt cgaacccgaa caattgaatt gcactagaag ctccatgcgg ccattcggct
     4081 gcatggagct gtgggccggc aatgagaggg cgcaccgctc tctggacctg gcaggattgc
     4141 aaagcgacgt catcgccgtg ccctccggcg gcgacaaagg cggcgatctt tccgcggtgt
     4201 tctcttgcag cgacaatacc gcccgcgtgg ttctcgcgga ttgcgtcggc cacggctaca
     4261 cggccagcgg agtggcccgg cacgtccacc accttctgca taaatttcaa gacattcgag
     4321 atacggcggg gttgctggca gcgctgaatg atgcatttac tctctcgaat gaaaaattgg
     4381 atggtccgtt gcggctgacg acggtcgtca caggaacttt tgacggcaca agcggcgaat
     4441 ttaattttgc ctatgcggcg cacccgcgga tgtttctctg gcgcgagcgc gagaagcgat
     4501 tcctggagtt gggagaaggg ttggaaggct acccgctggg ctacatcacg ggtgaaacct
     4561 acagccag
//
LOCUS       DASYVO010000466         8667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_235583, whole genome shotgun sequence.
ACCESSION   DASYVO010000466 DASYVO010000000
VERSION     DASYVO010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8667
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235583"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..673)
                     /locus_tag="VGR03_15875"
     CDS             complement(140..673)
                     /locus_tag="VGR03_15875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1995811.1"
                     /translation="MLDSQDRERFEQLVLPHLDAAYNLARWLLKNRADAEDVAQEALL
                     RALRFFRGFQGGDPRAWLLQIVRNTSYTWLDKHRPAELSVEFDEEMHVASIPTPEALA
                     IAGDNRDRLTRALEQLPPRFREILVLRELEECSYKEIAEITSIPIGTVMSTLSRARQR
                     LERELAGNAQQEASLEL"
     gene            885..1886
                     /locus_tag="VGR03_15880"
     CDS             885..1886
                     /locus_tag="VGR03_15880"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1995812.1"
                     /translation="MKKHAHGASKQTAAGEKDVLLHDHNNDGIDRRGFLQCMAWAGTG
                     VLCVVKGGVLESYSLSSLTELGPDQAKGELNFVQISDSHMGFNKPANTDVAGTLKAAV
                     EKINRLSVKPEFLLHTGDITHLSKPEEFDAVGQILRSASTTDMFFVPGEHDVLNDDGK
                     QYLERYGKGSQGAGWHSFDKKGVHFIGLVNVLNLKAGGLGTLGGEQLEWIEKDVKHLK
                     ASTPIVVFAHIPLWSVYPQWGWGTEDSAQALAYLKRFGSVTVLNGHIHQTMQKVEGNV
                     TFYTAASTAFPQPQPGKADSPGPMKVPADQLRGLLGITEVSYVRGKHALAVVDSKLG"
     gene            1937..2305
                     /locus_tag="VGR03_15885"
     CDS             1937..2305
                     /locus_tag="VGR03_15885"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEV1995813.1"
                     /translation="MRRGLRIALVAAAALGFVLWSGARSISGGGGELKPVHSAEAKAE
                     VKIDNFTFGPATVTVDKGSTVTWINRDDIPHTVVSSDKVFKSKVLDTDEQFSFTFAQA
                     GTYPYFCSIHPKMTGTVVVK"
     gene            2895..3815
                     /locus_tag="VGR03_15890"
     CDS             2895..3815
                     /locus_tag="VGR03_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995814.1"
                     /translation="MSRDARFERQMQPLPGRHRFLRELCLASVAVLATSASGATEISP
                     EPQLRADTATAFAHYVELTEARNSQELRAGSPVFWVDGLAEKERTAAYAELRRGQVEL
                     RRLETREADGKISCPHGLIHHWVGLIFIPGAKLDDVLALQQDYDHQEKYFAPDVERSR
                     IESRDGEHFRIFLRFRRHKVVTVVLNTQHDVRYFRDSPTRAHSRSSAVRIAQVENAGH
                     AREREKPEGNDDGFLWKMETWWRMEERDGGVYVQGEVASLTRDIPVGLGWLIGPFVTS
                     IPRETLTFTLEATRRAVESRRGKVPEAARR"
     gene            complement(3812..5050)
                     /locus_tag="VGR03_15895"
     CDS             complement(3812..5050)
                     /locus_tag="VGR03_15895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1995815.1"
                     /translation="MRLAVVSPFLDRRHGTERVVIEQLERLSALPGLEIHVYAQRLED
                     LAQAAPFDSSGRSQTPGSIVWHKVSSLPGPHLFGYIWWFFANQFHRLRDARFRGLTYD
                     LVYSPGINAWDADAIAIHIVFHEFYRQLRPYLRLSAAPLSRWPRLLHRRLYYWLIMAL
                     EGRIYRDPRTHLSAVSGVVARQLAVHFQRTDVPVISHGVDAEHFSSRVRLARRKTARS
                     HFGITAGEFVLLLIGNDWMTKGLPTLLRAIANCQNGSLKLIVVGTDQRSAFEALIRQL
                     SLTERVFFHPPSNDVLQFYAAADAYAGPSLEDAYGLPFLEAMACGLPVIASVRAGVSE
                     IMRDGVDGFLLRDPEDHMELAGRIRQLAGDPALRERMGTAAALTAQQHTWDRNAAETW
                     QFLKDAAARKQDARRRREAR"
     gene            complement(5052..6626)
                     /locus_tag="VGR03_15900"
     CDS             complement(5052..6626)
                     /locus_tag="VGR03_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995816.1"
                     /translation="MRALSTGILSPGRRGPLVGIGLFVLVLLAAYGASQYILSGDNFG
                     LVFIGAVAAAGAAAVAILNDWRKGLYIFLTWLLVEDLFRKFLGNNMAVYFGKDILVAF
                     MYISFLAAIRRMEVKTFRPPFLVPLSIFFAFGVLQVFNPASTSFILGALGMRLYFYYI
                     PLVFIGYALFESEADLRQFFSFNLVLAGVIGCLGILQAVLGHTFLNPSKPAEDIRELS
                     TLYRQAPLSHALAYRPTSLFVSTGRFATYMVLSWLAAFGFSGYLLLRHRRGRLLAFLV
                     LAITTVGVVMSSSRGAVLWTAGSALVGAAAFLWGAPWRQREVIRVLRLLQRTLLVAGI
                     ALTIFIIIYPEQIGASFAIYDETLSPDSPVSELVYRTRDYPLKNFLLAFDHPRWPYGY
                     GIGTASLGVQYVARIFHERSPVIGVENGYGTLVVELGIVGLILWILWTGALVISAWAV
                     VRKLKGSPWFPLAFMIFWYVFLLLFPITYAGINPYQDFVLNAYLWLLVGVLFRLPHIA
                     LSAQDEKRMQAASGTS"
     gene            complement(6623..7282)
                     /locus_tag="VGR03_15905"
     CDS             complement(6623..7282)
                     /locus_tag="VGR03_15905"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1995817.1"
                     /translation="MKLHTTTNDGLHDFVAGRVLARYAHPGVRAVDLGAGPGAMSERL
                     LVLGCDVVAVDRDPAGYAARPPFLALDFDQPDFASRLGPRKFELVTAIEVIEHVESAI
                     GFLRNIAHLLAPGGVAVLTTPNVDSLPARIKFLLAGKIRTMDDFGEPTHITPIFWDLL
                     GRQFLPRAGLKLREHYLFPPDGYQLSRKPIAWVLSVVAAALRGPVLLGDNHILVVEAA
                     P"
     gene            complement(7279..8451)
                     /locus_tag="VGR03_15910"
     CDS             complement(7279..8451)
                     /locus_tag="VGR03_15910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1995818.1"
                     /translation="MKLLIYSHYFAPSIGGVETIVGSLAGGLAECRTAAGAPEFEVTL
                     ITRTPVGDFEDRTMPFPVVRQPGFWELFRRVRGTEVLHVAGPALLPILFGMIARKPVV
                     VEHHGFLTICPNGQLVQEPSRSPCPGHFMAGHHAECWRCNASQGNLTSRKLWLLTFVR
                     RFLSARVAANIMPTQWLSRQLNLPRATCIPHGLEPFRAGPQRRSTNPIPVIAFQGRLV
                     TTKGIGVLLEAARILQEQSVDFTLRIIGDGPERNSLERLAEEKRISPRVQFLGGLPPP
                     KLEEELGQTDVIVVPSLGGEVFGLVIAENMLRGLPVVASDLGAFVEVIGDAGITFRTA
                     DASDLAHQLAELLCDSTVRADMIVRARKRGFSFFSKARMIEAHANVYRRLHLAANP"
     gene            complement(8448..>8667)
                     /locus_tag="VGR03_15915"
     CDS             complement(8448..>8667)
                     /locus_tag="VGR03_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995819.1"
                     /translation="PCRQMALKFPRIRGKLNMAMDCCNNKGAVLGIRIEMSPNVCAPA
                     EVILRKSNKPSSARHRPVPGRGQEELTA"
BASE COUNT         1982 a         2463 c         2697 g         1525 t
ORIGIN      
        1 gagcgccgtg tgtagcgttt gctgtgcttc cagcgcggag gcgcagtcgc ggcaaccctc
       61 gagatgctgt tcgaattccg ccgcccgcgc ggcgtccagc tctccatcca ggtagccgtg
      121 cacgatcgtt cgggattgtt cacagctcaa gggaagcctc ctgctgcgcg ttcccggcca
      181 gctcgcgctc caatcgttgc cgcgcgcggg aaagcgtgga catcaccgtg ccgatcggga
      241 tggaggtgat ttccgcgatt tctttgtagg aacattcctc gagctcccgt aggacgagga
      301 tttcgcgaaa acgcggcggc aattgttcga gggcgcgggt gagccgatcg cgattatccc
      361 ccgcgatcgc cagggcttcg ggtgtgggaa tggaagccac gtgcatctcc tcatcaaact
      421 caacggacag ctcggcgggc cgatgcttgt cgagccaggt gtagctggta ttacgcacaa
      481 tctgcagcag ccaagcgcgg ggatcgccgc cctggaatcc gcgaaagaag cggagcgcgc
      541 gcagcagggc ctcctgtgcc acatcttccg cgtccgcgcg attcttcaag agccagcgcg
      601 caaggttgta tgcggcatcg agatgcggca gcacaagctg ctcaaaacgc tcccggtcct
      661 gcgagtccaa cacgtcacgc ctttccccgg ccggaacgcc tcaaccagct agaccggcga
      721 acagggcatt ttattcccca ttcggggact taggacggga aatccccgca gcccgcaaag
      781 ggtggggtat ggcgggaata aaaccggggg tatggcagtc tagacggatg atgccctgcg
      841 cagtgggctg gcgtcaggga tcaggaatcc gaaaggagta agcgatgaag aaacatgcgc
      901 atggggcgag caagcaaacc gcggctgggg agaaggatgt gcttctgcac gaccacaaca
      961 acgatggcat cgatcgaaga ggcttcctgc agtgcatggc ctgggccggg acgggcgtct
     1021 tgtgcgtcgt caaaggcggg gtcctcgaat cctacagcct gagcagtctc accgaactcg
     1081 gcccggacca ggccaaaggg gaattgaact tcgtccagat cagcgacagc cacatgggct
     1141 tcaataaacc ggcgaacacc gacgtcgcgg gaacattgaa ggctgccgtg gagaaaatca
     1201 acagattgtc cgtaaagcct gaattcctgc ttcacaccgg agacatcacc catctttcca
     1261 aaccggaaga gtttgacgcg gtcggccaga tcctccgctc ggcgagcacc acggacatgt
     1321 tcttcgtgcc cggggagcac gatgtcctga acgacgacgg caagcagtat ctcgagcgct
     1381 acggaaaagg aagccaaggg gcaggttggc acagcttcga caaaaagggt gtgcacttca
     1441 tcggcctggt caacgtgctg aatctgaaag cgggagggct gggcacgctc ggcggagaac
     1501 aactggaatg gatcgaaaag gacgtgaagc atctgaaggc gagcacgccg attgtggtct
     1561 tcgcgcatat ccctctttgg tccgtttatc cgcagtgggg ttggggaacg gaggacagcg
     1621 cgcaggctct cgcatacctg aagcggttcg gatcggtgac ggtcttgaat ggccacatcc
     1681 atcaaacgat gcagaaggtg gaaggcaacg tcacattcta caccgcggcc tcgacggcct
     1741 tcccgcaacc tcaaccgggt aaggctgatt ccccgggccc gatgaaggtt ccggccgatc
     1801 agttgcgcgg cctcctgggg atcaccgagg tcagctatgt tcgcggcaaa catgccctgg
     1861 ccgtggtgga ctccaagctg gggtgaatcc gcctggatcg agggattgtt tcgaatttga
     1921 aaaaggagaa agtcgcatga ggagaggatt gcgaattgca ctggtggcgg ctgcggcttt
     1981 gggatttgtc ttgtggtcag gggcgcggag catctccggt ggcggagggg agctaaagcc
     2041 ggttcatagc gcggaagcga aggcggaagt gaaaatagac aatttcacct ttggcccggc
     2101 gacggtgacg gtagacaagg ggagcacggt cacctggatc aatcgcgacg acatcccgca
     2161 caccgttgtc agctccgata aggttttcaa gtccaaggtt ctggacacgg atgagcagtt
     2221 ctctttcacc tttgcgcaag ccggaacgta tccctacttt tgttcgattc atccgaaaat
     2281 gaccggcacg gtggtcgtta agtagcggcc ggctgcggtg gtcagattga ccgcgcacca
     2341 ctcagcatgc acttcggcgg gttcctgagc ggagggagtg gtccgcacta gagttagatc
     2401 cggcaagatt gaccattatc ctcctaaccc gttcattgca agtaagttat agaacgaagg
     2461 ctcctagctc cgttcgaact tgatgcgcct atgtgttcgt cgaaacaaac cgccatcgcc
     2521 ctgagagcac ggtcggtggg gtttagctcc aaaaagttgg aacggcacta gtaccccggt
     2581 accccattca gtggctcggg tgggccattg aggctcgcgc aacagaccgt taatttactg
     2641 aagacgggtt gggggccgcg cggggacagc agttccgcaa ggggctgtgt gcgagctccc
     2701 caacccgcca taatccttca agaaaccggt tctcctggtc tttatcttcc tgtcagcttg
     2761 catgcactcg caaaaggctc gagttctctg tcaaccaacg gcacaatgtt tgcaacattc
     2821 accggtggtg atagccggaa gagtgcaaga atcgttccgg cgacggccct tatcaatggg
     2881 ttgcggagaa accgttgagc cgagacgcca gattcgaacg acagatgcag cctctgcccg
     2941 gcaggcacag gttcctgcgg gagctgtgtc tggcctcggt cgcggttctc gcaacctctg
     3001 cgagcggcgc cacagagatc tctcccgaac cgcagttgcg ggcggacacc gccactgcct
     3061 ttgcgcatta cgtcgagctg accgaagcac gaaacagcca ggagctgcgc gccggttcgc
     3121 ccgtgttctg ggtggatggc ctggcggaga aagaacgaac ggcagcctac gcggagctgc
     3181 gtcgcggcca agtggagcta agacggctgg aaacgcgcga agcagatggc aaaatatcct
     3241 gtccgcacgg attgatccac cactgggtgg gtctgatatt cattcctggt gcgaagctgg
     3301 acgatgtcct cgccctgcag caggactacg accaccagga gaagtatttc gcgcccgacg
     3361 ttgagcggtc gcgcatcgaa tcccgcgacg gcgagcactt ccgcatcttc ctgcgcttcc
     3421 gaaggcacaa agtcgtcacg gtagtcctga acacgcagca cgatgtgcgt tattttcgcg
     3481 attcacccac gcgcgcgcac agccgcagct cggcggtgcg cattgcgcaa gtggaaaacg
     3541 ccgggcatgc ccgcgagcgg gagaagccgg agggtaacga cgacggcttc ctctggaaga
     3601 tggagacgtg gtggcggatg gaagaacgcg acggcggagt ctacgtgcag ggcgaggtgg
     3661 cgtcgctcac gcgggacatc ccggtgggtt tgggctggct gattggccct ttcgtgacaa
     3721 gcattccacg cgaaacgctt acgttcacgc tggaggcgac ccggcgcgcg gttgaatcgc
     3781 gccgcggaaa agtgccggag gcggccaggc gctagcgtgc ttcccgccgg cgccgggcat
     3841 cctgcttgcg cgccgcggcg tctttgagaa actgccaggt ctcggcagca ttgcggtccc
     3901 aagtgtgttg ctgggctgtc aatgcggctg ccgtccccat tctttcccga agcgccggat
     3961 caccagcgag ttgccgaatc cgcccggcaa gctccatgtg gtcctccgga tctcgaagga
     4021 gaaacccatc caccccgtcc cgcatgatct cgctcacgcc ggcccgcaca ctggcaatca
     4081 cgggcaaccc gcaggccatc gcttcgagga acggtaatcc ataagcgtct tccaaggaag
     4141 gtccggcgta ggcatccgcc gcggcgtaaa actgcaaaac atcgttagag ggaggatgaa
     4201 aaaaaacccg ctcggtcaga gaaagctgcc gaatgagagc ttcgaaagcg ctgcgctgat
     4261 cagtgcccac gacaatcaat ttcaaggaac cgttttggca gttcgcgatg gcgcgcagca
     4321 gagtggggag gcccttcgtc atccagtcgt tcccgatgag aagaagcaca aactccccgg
     4381 cagttatacc gaagtgcgat cgggcggtct tgcggcgcgc tagccggacc ctggatgaga
     4441 agtgctcggc atcaacgccg tgcgaaatga ccggcacatc cgtgcgctga aaatgaacgg
     4501 ccaattgccg ggcaacgacg ccggaaacgg cggaaagatg ggtgcgtgga tcgcgataga
     4561 ttctgccctc aagcgccatg atcagccaat aatacaatcg gcggtggagc agacgtggcc
     4621 aacgactgag cggagctgct gacaatcgca aataggggcg caactggcgg tagaactcgt
     4681 gaaatacgat gtgaatggcg atggcatccg catcccacgc gtttattccc ggggaataga
     4741 cgaggtcgta ggtaagcccg cggaagcgag cgtccctcaa acgatggaat tgatttgcaa
     4801 aaaaccacca gatgtagccg aaaaggtgcg gcccgggcag cgaagaaact ttgtgccaga
     4861 cgatacttcc aggcgtttgt gaccggccgg aggaatcgaa gggggcggcc tgtgcaagat
     4921 cctcgagccg ctgcgcgtaa acgtggatct ccagcccggg cagcgccgat agcctttcca
     4981 attgctcgat cacaacgcgt tccgtgccgt gacgccgatc cagaaaggga gagacaacag
     5041 caaggcgcat gtcagcttgt tccggaggcc gcctgcatcc gcttttcatc ctgggccgaa
     5101 agggcaatgt gggggagccg aaagagaact ccgacgagaa gccagagata ggcgttcagg
     5161 acaaaatcct gatatggatt gatgccggcg taggtgatgg gaaagagaag gagaaagaca
     5221 taccagaaaa tcataaatgc caaaggaaac cagggtgacc ctttgagttt gcgcacgaca
     5281 gcccatgccg aaatcaccag cgccccagtc cacagaatcc aaaggatcaa acctacaatg
     5341 ccaagctcca cgacgagcgt gccgtagccg ttctccaccc cgatgacggg agacctctcg
     5401 tgaaaaatcc gcgcgacgta ctgaacaccg agtgaggcag tgccgatgcc gtagccgtac
     5461 ggccaccggg ggtgatcaaa ggcaagcaga aaattcttaa ggggatagtc ccttgtgcgg
     5521 tagacaagct cgctgaccgg gctgtccggg gacaaggtct cgtcatagat ggcgaagctc
     5581 gctccaatct gttccgggta aataatgatg aaaatggtca aagcgattcc ggcaacaaga
     5641 agtgtgcgct gcagcaggcg caggacgcga atgacctcgc gctggcgcca aggcgcgccc
     5701 cagagaaaag cagcggctcc cacaagggcg ctgcccgccg tccagagtac ggctccgcga
     5761 gaggaactca tcacaacgcc gacagtggta atggccagga ccaggaaagc cagtagcctg
     5821 ccacgccgat ggcgaagcag gagatatccg ctaaatccaa atgcggcgag ccacgacaga
     5881 accatataag ttgcgaaacg cccggtgctg acaaagagcg acgtgggacg ataggcaagc
     5941 gcgtgagaga gcggtgcttg gcggtataaa gtactcaatt cccggatatc ttcagcgggc
     6001 ttggatgggt tcaggaatgt atgacccaga acggcctgca agattcccaa acatccgatt
     6061 acgcccgcca ggaccagatt gaaggagaaa aattgccgca aatcggcctc ggattcaaat
     6121 agggcgtacc caatgaaaac cagggggatg taatagaaat aaagcctcat tccgagagcg
     6181 cctaagatga aactggtgga agccgggtta aaaacttgca gcacgccgaa cgcaaagaag
     6241 atcgagagag gtaccagaaa gggcggacga aatgtcttca cttccatccg tcgaatagcc
     6301 gctagaaaag agatgtacat gaacgcgacg aggatgtctt tgccgaagta gacggccatg
     6361 ttgtttccga gaaacttgcg aaataggtct tcgaccagga gccaggtaag aaagatgtaa
     6421 agacccttcc gccagtcatt caggatggcg accgccgccg cgccggcggc cgcaacggct
     6481 ccgatgaata ccaggccaaa attgtccccg ctcaggatgt attgggatgc tccgtaggca
     6541 gccagcaaga cgaggacgaa caatccgatg ccgacaagcg gaccccgccg gccgggagag
     6601 agaatgcccg tggacagagc cctcatggcg cggcctcgac gacaagtatg tgattgtcgc
     6661 ccagaagaac gggcccccgg agcgctgcgg caaccacgct gagtacccag gcgatgggct
     6721 tgcggctgag ctgatagcca tcggggggga aaaggtagtg ttcccgaagt ttcaatccgg
     6781 cgcgtggaag aaattgccgc ccaagcagat cccagaagat cggcgtgatg tgcgtcggct
     6841 cgccgaaatc atccatggtg cgtatcttgc cggcgagcag aaacttgatg cgcgcgggaa
     6901 gcgaatccac gttcggggtg gtcaagacgg cgacaccgcc cggcgcgagc aagtgcgcga
     6961 tatttcgcag gaagccgatg gcgctctcga cgtgttcaat cacctcgatg gcggtgacca
     7021 attcaaattt ccgcggcccg aggcgggagg cgaaatccgg ctggtcaaag tcgagcgcca
     7081 ggaacggcgg ccgcgctgca tacccggctg gatcacggtc taccgcgacg acgtcgcagc
     7141 ccaagaccag cagccgctcg gacatcgcgc cggggccagc gccgagatcc acggcgcgca
     7201 cgccaggatg cgcatagcgg gcaaggactc ggccggcaac gaagtcatga agaccgtcgt
     7261 ttgtcgtcgt atgcaacttc atggattcgc cgcgaggtgc aatctgcgat atacgtttgc
     7321 atgcgcttca atcatacggg ccttggagaa gaaactgaat ccgcgtttcc gggcgcgcac
     7381 aatcatatca gcgcggacgg ttgaatcgca gagcagttcg gcaagctgat gtgccaggtc
     7441 cgaagcgtcc gccgtccgaa aggtgatccc agcgtctccg atcacctcaa cgaaggctcc
     7501 cagatccgag gcgacgaccg gcaaaccgcg cagcatgttc tccgcgatga caagtccgaa
     7561 cacttcgcct cccagcgaag gaaccacaat gacatcggtt tgccccagct cttcctccag
     7621 cttcggcggc ggcagtccgc cgagaaattg cacgcgcggc gagattctct tttcctcggc
     7681 caggcgctca agggaatttc gctcgggtcc gtcaccaatg atccggagtg tgaagtcgac
     7741 ggattgttcc tgcaaaatac gagcggcctc aaggaggacg ccgatgccct tggtcgtcac
     7801 cagacgcccc tgaaacgcaa tcaccggaat ggggttcgtg gatctccgtt ggggtcccgc
     7861 gcggaaaggt tcaaggccgt gcggaatgca ggtggcgcgc ggcaggttca actggcgaga
     7921 gagccattgt gtgggcatga tgttcgcggc aacccgagcg cttagaaacc ggcgaacaaa
     7981 cgtcagcagc cagagcttgc ggctcgtcaa attgccctga ctcgcattac atcgccagca
     8041 ctcggcatgg tgcccggcca tgaagtggcc ggggcaggga ctccgggacg gttcctgaac
     8101 cagttgtccg ttcgggcaaa ttgtcaggaa tccgtggtgt tcgacgacga ccggcttacg
     8161 ggcgatcatg ccgaagagaa tcggcagaag agcgggcccc gccacatgaa gaacttccgt
     8221 gccgcggacg cgccgaaaaa gctcccagaa cccgggctgg cgaacgacgg gaaaaggcat
     8281 tgtcctatct tcaaaatccc caaccggcgt ccgcgtgatt agggtcactt cgaattccgg
     8341 cgcgccggca gcggtgcggc attccgccag gcctccggcc agcgaaccca cgatggtttc
     8401 cacgcccccg atactgggcg cgaaatagtg ggaatagatc aggagcttca cgcggtgagt
     8461 tcttcttgcc cccgcccggg gacaggccgg tgccgcgcgg aggatggctt gttggatttt
     8521 cgcaggatga cttcagcggg cgcgcagacg ttgggagaca tttcgatgcg tattcccaga
     8581 actgccccct tgttgttaca acaatccatc gccatgttaa gcttgccacg aatgcgcggg
     8641 aatttcaggg ccatctgtcg gcacggt
//
LOCUS       DASYVO010000467         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_181129, whole genome shotgun sequence.
ACCESSION   DASYVO010000467 DASYVO010000000
VERSION     DASYVO010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181129"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VGR03_15920"
     CDS             complement(<1..449)
                     /locus_tag="VGR03_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995820.1"
                     /translation="MQKTTPFRISAVLFALLLLLGASVRAQQLADRPKQQAALRSLAY
                     DATQETVLEGTVLRYSAEAATPPIGAHLILETANGTIDLHLGGASYLQANHFSLAKGD
                     SVRVVGVNSATRQGSIFLVRVIQKGGQSLILRTVKGAPLSLAGARALG"
     gene            886..2556
                     /locus_tag="VGR03_15925"
     CDS             886..2556
                     /locus_tag="VGR03_15925"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV1995821.1"
                     /translation="MAPKYIFVTGGVVSSLGKGVAASSIGCLLESRGFKVTLQKCDPY
                     LNVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTHAKLSRDNNWTTGRIYETILTK
                     ERRGDYLGKTVQVIPHVTDEIKATIRKVSDAVDVVIVEIGGTVGDIESLPFLEAIRQM
                     RLELGAENTLFVHVTLVPYIAAAGELKTKPTQHSVKEMLSIGIQPDILLCRSDHHIPA
                     ELKKKIALFCNVVESCVISMEDVDTIYAVPAELSKEGLDEQILRLLRLESRPSDMKPW
                     LDLVYRMHHPAGEARIAIVGKYVQLEDAYKSLREALLHGGLAHDHKTVLEWIEAEEID
                     SAATAAARLHGFDGILVPGGFGKRGIQGMIHTIQYAREHKVPFFGICLGMQCATIEYA
                     RDAAGLKQADSTEFDPQTPHRVIYKLRELLGVDEMGGTMRLGAWPCKLEAGSFAHKAY
                     GKLEISERHRHRYEFNRDYEKTLVAAGLRITGRTPDENYVEIVEAPDHPWFLGCQFHP
                     EFKSKPLEPHPLFAAFIGAALENKKNKMRPVAEPKRVQAVGITPHVTA"
BASE COUNT          611 a          750 c          824 g          539 t
ORIGIN      
        1 cccagggcgc gagcgccggc gagggaaaga ggtgcaccct tcacggttcg caggatgagg
       61 gattgcccgc ccttttggat cacgcgaaca agaaagatgc tcccctgccg cgtggcggaa
      121 ttcacgccca cgactctcac cgaatcacct tttgccagtg aaaagtggtt cgcctgaaga
      181 taggaggccc ctccgagatg gaggtcaata gtgccgtttg cagtctctaa aatgagatgc
      241 gcgccgatgg gtggggtcgc agcttccgcg gaatagcgca ggaccgtgcc ttccagcacc
      301 gtctcctggg ttgcgtcata ggcgaggctg cgcagcgcag cctgctgctt cggccggtca
      361 gccaattgct gcgcccggac cgaagcgcca agaagcagca ggagcgcgaa taagacggct
      421 gaaattctga agggcgttgt cttctgcatc ccaagctccc ggggacgact ccactgcgcg
      481 ggctctgtca cttcgcagcc tgtggcgagc caacgggctt caagtcactc ccttttataa
      541 gaacccattc gcaccggaaa agttgcgcgg gtgtttcggg ccgtgcgcag catttccatt
      601 ctttagatgg ttgcgtccca aggatcggta cctggaaccc gtgaacatgt tctctgcgtt
      661 gaacgggcat atacgcatga tcgtggcgat tgaacgtgca cgaagtttga gaataggccc
      721 gatcgaaaaa ggcttcggag cgagagttca agaattccag aaaaaaattc gatttgaggg
      781 agcggcgagt tcgatttgac agaattgcga gttccgacga taattatgcg tacgtcactc
      841 ctgtggaggg ctgacaagcc tggcgtggga acgcgggcta cacggatggc gccaaaatat
      901 atttttgtaa ccggcggagt tgtttcctca cttgggaagg gcgtcgctgc ttcctcgata
      961 ggctgtctcc tcgaaagtcg cggtttcaaa gtcaccctgc agaaatgcga tccgtacctg
     1021 aacgttgatc cgggtacaat gagccctttt cagcatggtg aagtgtttgt gaccgaagac
     1081 ggagcggaga cggacctcga tctcggccac tacgagcggt tcacgcacgc caagctttcc
     1141 cgcgacaaca attggaccac cgggcgaatc tacgaaacga tcctgacgaa ggagcggcgc
     1201 ggagattatc tcgggaaaac cgtgcaggtg atcccgcacg tgaccgacga gatcaaggcg
     1261 acgatcagga aagtctctga tgccgtcgat gtcgtgattg tcgagattgg cggcacggtt
     1321 ggtgacatcg aatcgcttcc cttcctcgaa gcgattcgcc agatgcggct cgaactgggg
     1381 gcggagaaca cgctctttgt acacgtcacg ctggtgccgt acatcgccgc ggccggggag
     1441 ctgaaaacca agccgacgca gcacagcgtg aaggaaatgc tgagcatcgg gattcagccg
     1501 gacattctgc tctgccgcag cgaccaccat attccggcgg aactgaagaa gaaaattgcg
     1561 ctgttctgca acgtcgtgga atcctgcgtc atctcgatgg aagacgtgga cacgatttac
     1621 gcggtgcccg cggaactgag caaagagggc ctcgacgagc agattttgcg cctcttaaga
     1681 ctggaatcgc gcccctcgga tatgaagccg tggcttgatc tcgtctaccg gatgcatcac
     1741 ccggcaggtg aggcgcggat tgcgattgtc ggcaagtacg tgcagctcga agatgcctac
     1801 aagagtttgc gcgaagcctt gctgcacggc gggctggcac acgatcacaa aaccgttctc
     1861 gaatggatcg aagcggaaga aattgattcg gcggcgaccg ccgcggcgcg cttgcacggc
     1921 tttgatggaa tcctcgttcc cggcggcttc gggaagcgtg gcattcaggg gatgatccac
     1981 acgattcagt acgctcgcga acacaaggtg ccgttttttg ggatttgcct ggggatgcaa
     2041 tgcgccacca tcgaatatgc gcgcgacgcg gccggattga agcaggcgga cagcacggaa
     2101 tttgacccgc aaacgccgca ccgtgtgatt tataagctgc gcgaattgct cggggtcgat
     2161 gagatgggcg gcacgatgag actgggtgcg tggccctgca aacttgaagc gggctctttc
     2221 gcgcacaagg cttacggcaa actggagatc agtgagcgcc accgccaccg ctacgagttc
     2281 aaccgcgact acgaaaaaac tctggtcgcc gccggactgc gcatcacggg acgcacgccg
     2341 gatgagaatt acgtggaaat cgtcgaggcg ccggaccacc catggttcct cggctgccag
     2401 tttcacccgg aattcaaatc gaagccgctg gagccgcatc cgctgttcgc ggcgttcatc
     2461 ggcgcggcgc tggaaaacaa aaagaacaaa atgcggcccg tcgcggagcc caagcgcgtc
     2521 caggctgttg ggatcacgcc tcacgttacc gcctaatctg aatatttccg aactgagcgg
     2581 aagatgcccg caacatccgc gctcaaacaa aagagttcag tggcctggat agtttgattt
     2641 ttcttgcaag cgcagattga ttgcgcagtg gtagacgcca tctttttccc gcgagagccg
     2701 ccgaatcgtt gcaaacctcc caag
//
LOCUS       DASYVO010000468         3843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_138772, whole genome shotgun sequence.
ACCESSION   DASYVO010000468 DASYVO010000000
VERSION     DASYVO010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138772"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1963
                     /gene="asnB"
                     /locus_tag="VGR03_15930"
     CDS             <1..1963
                     /gene="asnB"
                     /locus_tag="VGR03_15930"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEV1995822.1"
                     /translation="LRMVPEGIVQRMAQALVHRGPDEDGFLQRPGVALASRRLSIVGL
                     ADGRQPIANEDRSVFVVFNGELFDYVEKRAELAGRGHRLVTHCDTEIIPHMWEENQEG
                     MFERLRGQFAVALWDVRRHQLILSRDRFGICPLYWTRQGDWLLFASEIKGLLASGMVP
                     AKPDPRGIDHIFTFSAMPGPVTCFEGVQLLPAGHYLQITPGNEDGAAPVIDERTYWEM
                     DFPDEGDEDPGANQRKLVDEFERVMLQAVEKRLRADVPVGAYLSGGVDSSMIVALACH
                     LKGPAINTYTIRVDDPALDELSAACLAARHIGTKLPVVQEFRASDALETYPALIQAAE
                     APVIDTSCAALLQLARRVHSCGQKVVLTGEGADEWLVGYPWYKAAKLLGYLDILPGLP
                     LSDRARRAYFRVNQVPQYSVEMRQRAEQSIGGPNAWIDAYGMLALCKLRFYAAPMREV
                     LEQTNPWADLQLNLERAKRWHPLNRGIWVAGRVTLAGHLLQAKGDRVAMHSSVEVRYP
                     FLDEDVFDFLAKLHPCWKLRGFRDKHLLRLLAERWLPPSIYRRGKVIFRAPLDSFHID
                     PEPPFVGQLLSEESLRRTGYFDIAAVLHWQRAFREMRAGSLPRLSVEMGLVAVVATQL
                     WHHLFIDGALSDLPTWTGLAAAANAAPQA"
     gene            complement(1970..2740)
                     /locus_tag="VGR03_15935"
     CDS             complement(1970..2740)
                     /locus_tag="VGR03_15935"
                     /inference="COORDINATES: protein motif:HMM:NF045274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995823.1"
                     /translation="MRLSFSIKAFSVVTALLLTCAASRAQSADDLIEKGDVFALKFQA
                     DEALKDYLPAEKLEPKNVRLLVRISREYRHLMSDAAKTEEKLRLGGIAVDYAKRSAVL
                     GPNNPEAQLAVAISYGKLQPLVSTKEKVETARIIKSEADKAIKLAPRSDLAWHVLGRW
                     NMGYAEVTGVKRALAELIYGKLPATTYEDAAKCFEKAIELKPDRLIHYIELGLVYSHM
                     GRTADARRVITKGLGMKETEKDDPEAKQRGRETLAKLR"
     gene            complement(2821..>3843)
                     /locus_tag="VGR03_15940"
     CDS             complement(2821..>3843)
                     /locus_tag="VGR03_15940"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV1995824.1"
                     /translation="ELARERNVQIVGKTNLTEFAVTVSGKNSYFLTPRNRPDGKHKFI
                     PGGSSSGSAVAVQTGMADVAFGTDTAGSIRLPAACCGILGLKTTFGLVPIKGVFPISP
                     KHLDTVGPMAKDMPHLVQGMDLLQRGFAAQYESAVAANPSAKDIRIGRLYLDGTDPAI
                     DKALDDALAAKRFKVIRLDQAFKAKWEQAQKDGKTVAFADVWFNDQKYTDKKGVRGTT
                     KTIINLGRLDSTNNYKNALKRQAAWQHDLRQVFKKVDFIALPTLQKLPPKFPFFGRTV
                     VFELQVFSTQNTAAVNFAGNPALAMPIPMPVKGKTAPVTSLQLIGPRLSEAELLNAGR
                     LIEAGR"
BASE COUNT          695 a         1157 c         1151 g          840 t
ORIGIN      
        1 gctgcgaatg gtgccggagg gcatcgtcca acggatggcc caggcgctgg tgcatcgcgg
       61 gccggacgag gacggctttc tccagcgtcc gggagtcgcg ctggcttcgc gccggctcag
      121 catcgtcggg ttggcggatg ggcggcagcc gatcgccaat gaagaccgga gcgtctttgt
      181 ggtcttcaac ggcgagttgt ttgattatgt tgagaagcgg gcggagctag cagggcgcgg
      241 acatcgcttg gtcacccact gcgacacgga aatcattcca cacatgtggg aagagaatca
      301 ggaaggcatg ttcgagcgtc tgcgcggcca attcgccgta gccttgtggg acgtgcgtcg
      361 gcatcaattg attcttagtc gcgaccgctt cggcatctgt ccgctttact ggacgcgaca
      421 aggggactgg cttctgttcg catccgaaat caagggcctg ctggcctcgg gtatggtgcc
      481 ggccaaaccc gacccgcgcg gcatcgacca cattttcacg ttctccgcga tgccggggcc
      541 ggtgacgtgt tttgaaggcg tgcagcttct gcctgccggc cattatctgc aaatcactcc
      601 ggggaacgaa gacggcgcgg cgcccgtcat cgacgagcgt acctactggg aaatggattt
      661 cccggatgag ggcgatgaag atccgggagc aaatcagcgc aaactggtgg atgaattcga
      721 gcgcgtcatg ctgcaggcag tcgagaagcg gttgcgggcc gatgtgccgg tgggcgctta
      781 tttgtcgggc ggcgttgatt cgagcatgat cgttgcactg gcgtgtcatc tcaaaggtcc
      841 agcgattaat acctacacca ttcgcgttga cgatcccgct ctcgacgaac tcagcgcggc
      901 ctgtctcgcg gcccggcaca tcggcacgaa gctgccggtt gtgcaggaat ttcgcgccag
      961 cgacgcgctg gagacctacc ccgcgctgat ccaagctgca gaggcgccgg taatcgatac
     1021 gtcgtgcgcc gcattgttgc agttggcccg gcgcgttcat agttgcgggc agaaggtcgt
     1081 gctgaccgga gagggcgcgg acgaatggct ggtcggctat ccgtggtata aagcggcgaa
     1141 gctactcgga tatctcgaca tcctacccgg actgccgctc agcgaccgcg cgcgccgcgc
     1201 ctatttccgg gtgaatcagg tacctcaata ttcagtcgag atgcgccaac gcgcggagca
     1261 atccatcggc ggccccaacg cctggattga tgcttacggg atgctcgcgc tctgcaagct
     1321 tcgcttctac gccgcgccga tgcgggaagt gctcgagcaa accaatccgt gggccgatct
     1381 tcaactcaac ctcgagcgcg cgaaacgctg gcacccgctg aaccgcggca tttgggtcgc
     1441 aggccgcgtc acactcgcgg gtcacctgct ccaggccaaa ggggaccgcg tggccatgca
     1501 ctcgtcggtg gaagtgcggt atccgtttct cgacgaggac gtcttcgatt ttctcgcaaa
     1561 gctgcacccg tgctggaagc tgcgcggatt ccgcgacaaa cacctcctgc ggctgctggc
     1621 cgagcgctgg ctgccgccgt ccatttatcg gcgcggcaaa gtcatctttc gcgcgccgct
     1681 ggacagcttc cacatcgacc cggagccgcc gttcgtcggg cagctcctga gcgaagaatc
     1741 gctgcggcgc acgggctatt tcgatatcgc ggcggtgctg cactggcagc gggcctttcg
     1801 cgagatgcgg gccggttcac tgccgcgtct ctcggtggaa atgggactcg tggctgtcgt
     1861 ggcgacgcaa ctctggcatc acctgttcat cgacggcgcg ttgtcggact tgcccacgtg
     1921 gacgggcctc gccgcggcag cgaacgcagc accgcaggct tgaccgggct cagcgaagtt
     1981 tcgccagcgt ctcgcgtccg cgctgcttgg cttcggggtc gtctttctcc gtctccttca
     2041 tccccagtcc tttggtgata acgcgccggg catcggccgt tcggcccatg tgagaataaa
     2101 ccagccccag ctctatatag tgtatgagtc ggtcgggctt cagttcgatc gctttctcaa
     2161 aacatttggc tgcatcctcg taagtcgtcg ccggcagctt gccgtaaatc agttccgcca
     2221 acgcccgctt cacgcccgtg acctcggcat accccatgtt ccagcgtccc agcacatgcc
     2281 aggctaaatc gctgcgcggg gcgagtttga tcgccttgtc cgcctcgctc ttgatgatgc
     2341 gcgcggtctc caccttctct ttggtgctca ccagcggctg cagcttgccg taactgatcg
     2401 ccactgccag ttgggcttcc ggattatttg gccccaagac tgcggagcgc ttcgcatagt
     2461 caacggcgat cccgccgagc cgaagcttct cttctgtctt cgcagcgtcg ctcatcaggt
     2521 gccgatactc gcgcgagatc cgcacgagga gacgcacgtt cttcggctcc agtttttccg
     2581 ccggcagata gtccttgagc gcctcgtcgg cctggaattt tagagcgaag acatcgccct
     2641 tttcaatgag gtcgtccgcg gactgagccc ggctcgccgc gcaggtcagc agcagtgccg
     2701 tgacgacaga aaatgctttg atggaaaaag aaagacgcat ggttctgcaa atcgttaggc
     2761 cgacaccttc cactccctgc accgatgata gctccacagc cgttgcggtc gattaggtca
     2821 tcagcgcccg gcttcgatca ggcggcccgc gttgagaagt tccgcctcgc tcaaccgcgg
     2881 tccgataagc tgcaggctgg tgacgggagc ggtctttcct ttcactggca tcggtatcgg
     2941 catggccagc gcaggatttc ccgcaaagtt taccgccgcg gtgttttgcg tgctgaacac
     3001 ctgcaattca aacaccacgg ttcggccgaa gaatggaaat ttgggcggca gcttctgaag
     3061 cgttggcagg gcgatgaaat caaccttctt aaagacctgg cggaggtcgt gctgccacgc
     3121 agcctgcctc ttcagcgcat tcttgtaatt gttcgtggaa tcgagtctgc ccagattgat
     3181 gattgtcttg gtcgtgcccc ttactccttt tttgtccgta tatttttgat cgttgaacca
     3241 gacgtcggca aatgccaccg tttttccgtc cttctgagcc tgctcccatt tcgccttgaa
     3301 ggcttgatcg agtcttatga ccttaaatcg ctttgccgcg agagcatcgt cgagggcttt
     3361 gtcaattgcc gggtcggtgc cgtcaaggta aagccgccca attctgatgt ctttggccga
     3421 agggttggct gcgacggcac tttcgtattg ggccgcaaac cccctctgca ataagtccat
     3481 gccttgcaca aggtggggca tgtctttggc catgggcccg acggtatcca aatgctttgg
     3541 tgaaatagga aacacgcctt tgatgggaac caggccgaag gtcgttttca agcccaagat
     3601 gccgcaacaa gccgcgggaa ggcgaatgga cccggccgta tcggttccaa aggcaacgtc
     3661 cgccattccg gtttggaccg ccaccgccga accgctcgac gaacctcctg gaataaattt
     3721 gtgtttgcca tccgggcgat ttctcggagt tagaaagtag ctgttcttgc cagaaaccgt
     3781 gacggcaaat tcagtgagat tcgttttacc cacaatctgc acgtttcgct cccgtgccag
     3841 ctc
//
LOCUS       DASYVO010000469         3559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_7939, whole genome shotgun sequence.
ACCESSION   DASYVO010000469 DASYVO010000000
VERSION     DASYVO010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7939"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VGR03_15945"
     CDS             complement(<1..626)
                     /locus_tag="VGR03_15945"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995825.1"
                     /translation="MYYGRGRGQSHGGHQDARTRSTRGHHPNWGHPDHDACGTGFIAR
                     LGGAPSYDIIQFALTALERLTHRGGVDADGASGDGAGLLTSLPQAFFRARAQEQGIEL
                     PELFAVGFAFLPSSVVEEARAAVEASAETERLRVLGWRRVPVNTNSLGRRALETMPEI
                     WQFFVEPFHPAGATARFEWRLALLRKRAESLLPPRCYICSLSSQTIVY"
     gene            complement(722..2125)
                     /locus_tag="VGR03_15950"
     CDS             complement(722..2125)
                     /locus_tag="VGR03_15950"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEV1995826.1"
                     /translation="MKPATKTKKTSTVPRAGAAELLPERELRRIVDTVLRLAKSTGAE
                     ETEVHVDEVANALTRFANNAIHQNVAEHGLTVSIRTVADGRTARATTNRMDEDSLRAA
                     IEASLSLAHSQPKDPRLLPMPRKQRYRAVNRFIKPTAALSAEERARAVRRACDLAVKK
                     GQVAAGIYSNGQSQSAMGNSRGLFAAYRETHSEFSITAQEDSASSWAKANSADLSAFD
                     PQKLATRASEKAHLAVNARELAPGRYTVILEPAAVLDLVGFLFYDFAATALEDKRSCL
                     NERMGKQLFGPNISISDDVYHPLQQGAPFDGEGLPRQRELLVDRGIPRNLVYSRVAAK
                     AANKQPTGHGFALPNEYGEAPMNLVFGGGDSSIEKMIVSTERGLLVTRLWYIREVDPY
                     EKIMTGMTRDGLFLVENGLVTGAVRNFRFNQSLIEMLKNVELLGPAVRATGEEAFEMV
                     VPAMKVRDFCFSEVTKF"
     gene            complement(2122..>3559)
                     /locus_tag="VGR03_15955"
     CDS             complement(2122..>3559)
                     /locus_tag="VGR03_15955"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEV1995827.1"
                     /translation="LDTAKLRGAAYGDVRVMHLRQRDLTTKNGHVGTLSQSESIGLGI
                     RVLANGAWGFASTDRLTREGVAACAAQAVSIAKASALAKRSEVVMATEKAYVDSWQSP
                     FRKDPFEIPLETQLALLLAADAEMRRVKGVTLTETDMQFRKIDSWFASSIGSRIHQRK
                     VISGCGIAATSFQGEEIQKRSYPNSFGGQHSLQGYELVESLDLMSNAPVIAEEAAALH
                     SAQQCPEKVGTLILGGSQLGLQIHESIGHPIELDRVLGQEANFAGTSFLTLDQLNRLK
                     YASSIVNVVADARLDHGQGLGTFAYDDEGVPAQCTDIIKDGEFRGYLSNRETAHLVGL
                     DRSSGTMRTESWNRLPIIRMTNVSLLPGTWDYDDLIADTHDGILMETNRSWSIDDRRY
                     QFQFSTEIGWEIKGGKKVRMLKNPSYSGITTEFWNSCDAICKFDHWTLWGTPNCGKGQ
                     PMQTMGTGHGASPSRFRNVRIGTAFSNQ"
BASE COUNT          712 a          997 c         1113 g          737 t
ORIGIN      
        1 ttgtacacga tggtctgcga ggaaagcgag cagatgtaac atcgcggcgg caagagcgat
       61 tccgcccgtt ttctcaagag agcaagacgc cactcaaatc gcgccgttgc ccccgccgga
      121 tgaaatggct cgacgaaaaa ctgccaaatc tcgggcatgg tttcgagcgc gcggcgaccc
      181 aaagaattgg tattcaccgg cacccggcgc catccgagga cacgcagccg ctcggtttcc
      241 gccgaggctt caaccgccgc acgggcttct tcaaccacgg aagaaggaag aaaagcaaag
      301 ccgaccgcga acagttcggg cagctcaatt ccctgctctt gagcgcgggc gcggaagaag
      361 gcctgaggga gcgaagtgag caaaccggcg ccgtcgccgc tagctccatc tgcgtcgacc
      421 cctccgcgat gggtgagacg ctctaatgcg gtcaaagcaa actggataat gtcgtagctg
      481 ggagcaccac cgaggcgggc gatgaagccc gtgccgcagg cgtcgtggtc cggatggccc
      541 cagttgggat gatgaccccg agtcgaccgg gttcgcgcat cctgatgacc gccatggctt
      601 tggcctcgac cgcgcccgta gtacatacca gtttattaaa cgggtagagg gtggatgtac
      661 aacgcagaat tttgattctc tgaattgatt ttttgataag tgggctcgaa ggttcaaatc
      721 gctaaaactt ggtcacttcg gagaaacaga agtccctaac tttcattgcc ggcacgacca
      781 tctcgaaggc ttcttcgccg gtggcacgga cggcggggcc cagcaattcg acgtttttga
      841 gcatttcaat gagcgactga ttgaagcgga aatttcgcac tgcgcccgta actagcccat
      901 tttcaacgag aaacaggcca tcgcgggtca ttccggtcat gatcttttcg tagggatcga
      961 cttcacggat gtaccagagg cgggtcacga gtaaaccgcg ctctgtggaa acaatcattt
     1021 tctcgatcga cgaatctccg cctccgaaga cgagattcat gggcgcctcg ccgtattcgt
     1081 tgggcagggc aaagccgtga ccggtgggct gcttgtttgc ggcttttgcg gccacgcggg
     1141 aatagacaag attcctgggt atgccgcgat ccacgagcaa ttcccgctga cgcggcaagc
     1201 cctcgccatc gaacggagcg ccctgttgca gcggatggta aacgtcgtcg ctgatgctga
     1261 tgtttggtcc aaagagctgc ttgcccatgc gttcgttcag gcaggagcgc ttgtcttcaa
     1321 gagcggtggc ggcgaaatca taaaaaagaa acccgacgag atcgagcacc gctgcgggtt
     1381 cgaggatgac cgtgtaacgt ccgggcgcaa gctcgcgcgc gttgacggca agatgtgctt
     1441 tctcggaggc ccgcgtggcg agtttctgtg ggtcaaaggc gctgaggtca gcggagttgg
     1501 ctttggccca gctcgaggca gagtcttctt gcgcggtgat ggaaaactcg gagtgcgttt
     1561 cgcgataggc cgcaaacagg ccgcgcgaat tgcccatggc ggactggctt tggccgttcg
     1621 aatagattcc agcggcgacc tggcctttct tgacggccaa gtcgcaggcg cggcggactg
     1681 cgcgggctcg ctcttcagcg gagagagcgg cggtcggctt gatgaaacgg tttacggctc
     1741 gatagcgctg cttccggggc atgggcagga ggcgcggatc cttgggctgg ctgtgcgcga
     1801 gggagagaga ggcttcgatc gcagcccgga gcgaatcttc atccatgcga ttcgtggtag
     1861 cgcgcgcggt tcgtccatcg gcgacggtgc gaatcgaaac ggtcagccca tgctcggcca
     1921 cgttttggtg aatggcgttg ttggcgaaac gagttaaggc gttcgcaact tcgtccacgt
     1981 gcacttccgt ctcttcggct cccgtggact tcgccagacg aagaacggta tcgacgatgc
     2041 ggcgaagctc gcgctcgggg agaagctccg cagcccctgc tctgggcacc gttgatgttt
     2101 tcttcgtctt ggtggccggt ttcattggtt agaaaatgcc gtcccgatgc gcacgttgcg
     2161 gaaacgcgac ggcgaagctc catggcccgt gcccatggtc tgcatgggct ggccttttcc
     2221 gcaattgggc gtgccccaca gcgtccagtg atcgaacttg cagatggcgt cgcaggaatt
     2281 ccagaattcg gtggtgatgc cgctgtagct gggattcttc agcatgcgaa ctttctttcc
     2341 gcccttgatc tcccatccga tttccgtgga aaactgaaac tggtagcggc ggtcatcaat
     2401 ggaccaagag cgattcgtct ccatcaggat tccgtcgtgg gtgtcggcga ttaaatcgtc
     2461 gtagtcccac gtgccgggca gcaagctgac gttcgtcatt cgaatgatcg ggaggcggtt
     2521 ccagctttcc gtgcgcatag ttccggaaga gcggtccagg ccaacaaggt gcgccgtttc
     2581 gcgattcgaa agatacccgc ggaattcccc atctttgatg atgtcggtgc actgggcggg
     2641 aacgccttcg tcgtcgtacg caaaagtgcc gagtccctgc ccgtgatcga gccgggcgtc
     2701 cgcgacgacg ttcacaatgc tcgaagcgta tttcagccgg ttcaactgat cgagcgtcag
     2761 gaaactcgtg cctgcaaaat tggcttcctg accaagaacc cgatccagct cgatgggatg
     2821 gccgatcgat tcgtgaatct gcaacccgag ctggctgccg ccgaggatga gcgtgccgac
     2881 cttttccggg cattgctgcg ccgaatggag cgcggcagct tcttcggcga tgaccggggc
     2941 gttgctcatc aaatcgagag actcaacgag ctcgtaacct tgcagcgaat gctggccgcc
     3001 aaaactgttg ggataagaac gcttttgaat ttcttcacct tgaaacgaag tggcggcaat
     3061 tccgcagccg gaaatcactt tgcgctggtg aatgcgcgag ccgatgctgg acgcaaacca
     3121 ggaatcgatc ttgcggaatt gcatgtccgt ttccgtcagc gtcacgcctt tgacgcggcg
     3181 catttcggcg tcggccgcaa gcagcagggc cagttgcgtt tcgaggggaa tttcgaaggg
     3241 gtccttgcgg aaggggctct gccaactgtc cacgtaggct ttttccgtgg ccataacgac
     3301 ctcgctgcgc ttcgcgaggg ccgaggcctt cgcgatggaa acagcctgag ccgcgcaagc
     3361 cgcgacgcct tcgcgcgtca aacgatccgt ggaggcaaag ccccaggcgc catttgccag
     3421 cacgcgaatg ccgaggccga tcgattcgct ctgggaaaga gtgccgacgt ggccgttctt
     3481 tgtggtgagg tcgcgctggc gcaaatgcat cacgcgaacg tcgccgtagg cggcgccgcg
     3541 cagcttggcg gtatcaagt
//
LOCUS       DASYVO010000470        10383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_97339, whole genome shotgun sequence.
ACCESSION   DASYVO010000470 DASYVO010000000
VERSION     DASYVO010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10383
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97339"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..774)
                     /locus_tag="VGR03_15960"
     CDS             complement(298..774)
                     /locus_tag="VGR03_15960"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEV1995828.1"
                     /translation="MRVAVLTISDSVTKGEREDLSGPAVVAFCRGLGWEVTSTLHVSD
                     DPANIREQLRAVADSGRVDVLLTTGGTGIGPRDNTPEATQAVADRIVPGLSEEMRRKG
                     LEKTPTAVLSRGTAAVRTKTLIVNLPGSPKGAVESLEAVAHLLPHAVKVLHGARHD"
     gene            complement(797..1555)
                     /locus_tag="VGR03_15965"
     CDS             complement(797..1555)
                     /locus_tag="VGR03_15965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV1995829.1"
                     /translation="MSTAGHKTVKLLLADDNPLVRDLIVKGLEPFCEVETSTDGADAL
                     LKVVDAPPDVILCDYKMPGLDGRQLFEKLRARESTRHIPFLFMASRPDIEERLRPLVD
                     GVEDFITKPFLIKDLVRIAKKVVDRLHLEKLQKKASRPGVIQGRLEEMSMIDLMQSLE
                     MGQKSCRLIVQHDGAQCELYFASGQCRDAKIGSVEGDDAVYKVVLWTAGEFEIDFNAA
                     NASTKTTTTRNTTGLLMEAMRLMDEANRDTVGTP"
     gene            complement(1552..2046)
                     /gene="moaC"
                     /locus_tag="VGR03_15970"
     CDS             complement(1552..2046)
                     /gene="moaC"
                     /locus_tag="VGR03_15970"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006870.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEV1995830.1"
                     /translation="MAKLSHYGKKGEVRMVDVSEKAVTTRTAAARGFVKMKPRVVSAV
                     RRLKNPKGNPLEVARIAGIAAAKKTAEWIPLCHPLPLTHIDVTARLCQNGVEVRSTVT
                     TAAQTGVEMEALVAVAAAALTVYDMCKALDKGMEITDIVLTEKTGGKSGDFHRRKAAR
                     RRAR"
     gene            complement(2057..3268)
                     /gene="glp"
                     /locus_tag="VGR03_15975"
     CDS             complement(2057..3268)
                     /gene="glp"
                     /locus_tag="VGR03_15975"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEV1995831.1"
                     /translation="MLTYEQARNKVIEQVAKRKGPRATAVVSVWDALGLVLAQEVQTD
                     REYPPFDRSTRDGYVLRSKEAAAGTRLKCVGEIKAGDTVSETLAAGTCLQIMTGAAVP
                     AGADAVVMIEHTSREGDLVRFERAAQPGQNIVPRGSEAAAAQIILSPGMRLGYAELAL
                     AAQVGAVHLQCAQRPRVAILSTGDEVVLIDETPGPFQIRNSNSVSLAAQVRIAGGEPI
                     VLGNAADRIEDLGEKIERGLKEDALVLSGGVSMGKYDLVESVLKAMGAEFFFDAVAIR
                     PGKPAVFGMCEGKPVFGLPGNPVSTMVTFELFVAPAIDLLSGAEARALPMVEARLAEA
                     LNEKPGLAHFLPARVEWRGAVPEVKALKWQGSGDIAALAKANCFLVVPADREKIETGE
                     SVSLLLRKDVV"
     gene            complement(3369..4667)
                     /locus_tag="VGR03_15980"
     CDS             complement(3369..4667)
                     /locus_tag="VGR03_15980"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV1995832.1"
                     /translation="MKSVVVVGAQWGDEGKGKVVDYLAGSFDYIARVAGGHNAGHTVI
                     IGKDRYVLQLIPCGILRPKQHAVIGTGTVVDPAALVAEIAMLSKAGIDVKGRLHVSNR
                     AHLIFPYHRELDKAAEGARGANKIGTTSRGIGPAYEDKMARRGLRMCDLLDPELFRKK
                     AAHVVAEKNRLAKGAYGADIDFSHEVEETLKLGEKIRGYICDTAELLNRALDEGKSVL
                     FEGAQGTMLDIDHGTYPYVTSSSATSGGAATGVGVPPTKIKHVLGISKAYTTRVGGGP
                     FPTEMPDLDAKEVRERGKEFGAVTGRPRRCGWLDLQVLRYAKMINGIDSMVITKLDVF
                     DTQREIQVCVGYKYKGTPLKEMPALAEEYEHGTPEYKTLPGWNETTYGVKDGAKLPKA
                     AIDYLRFISDFLQVEIGMISTGPERDATIVPAGTLLARWL"
     gene            complement(4843..5427)
                     /locus_tag="VGR03_15985"
     CDS             complement(4843..5427)
                     /locus_tag="VGR03_15985"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV1995833.1"
                     /translation="MTASHKITRVRCAWAKNELNIPYHDEEWGVPVHDEQKWFEFLIL
                     EGAQAGLSWDTILRKRGRYREVFDAFDPVKVARYEKKKVRELLRDAGIIRNRLKIGAA
                     IGNARAFLKVQEEYAAFDAYIWRFVDGKAKQNAWKNHKQVPAKTAESDALSKDLQKRG
                     FRFVGSTICYALMQATGMVNDHLVTCFRHRDLSK"
     gene            complement(5459..7216)
                     /locus_tag="VGR03_15990"
     CDS             complement(5459..7216)
                     /locus_tag="VGR03_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995834.1"
                     /translation="MHQLRRSQAEQRDPLSPAGALLEPHPRIFFWCVILVLGLLNVWA
                     RRNDVSPDSISYIEIAWATARFGLHQIVNAYWSPLYPFLLSLVFRYFHPPMQWEFTAA
                     HLLNFAVYLASLASFELFLKELILQRQSSGESTEKSLPVSPRTVWIWGYVFFLWASYF
                     WLGPVWVTPDLCVAALVYLATALLFRIWRGRGSWLVFVGFGALLGLGYLAKTAMFPLS
                     FVFLSCAFCLCRIAGASFRGAALRTLLATAVFAGFALPLVLALSAAKERLTFGDSARI
                     SYTEYIDRATKTVHWQGEPLGTGNPAHPTRRIFSQPAVFEFASPIQGSYPPWYDPSYW
                     YEGVRPHFLLKWQLWALFRAANLYFKIFSKSGALWVVLVAVWVVGRRALAWGSSSSGA
                     WLVVLPSVAALAMYSLVLVEFRYVAPFALMLMLWMLARMRIVTGAEPQLLKRFHLVVI
                     LAPALAVAWPVARDLYDVIRNKPYEPWVVAQQLNAMGILPGTDVAYVGTGLDAYWAHL
                     AGVRIIVEIPDIEQPRFVTADAARRQQVIALFSSVGARAVVTKNADAAHPADGWRQIP
                     GTHHFIWQQPWLIAVPEKK"
     gene            complement(7356..7694)
                     /gene="trxA"
                     /locus_tag="VGR03_15995"
     CDS             complement(7356..7694)
                     /gene="trxA"
                     /locus_tag="VGR03_15995"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1995835.1"
                     /translation="MADNSIQAATDGNFEAEVLTASNSQPVMVDFWAEWCRPCHMLAP
                     TVAEIAHDYAGKLKVMKLNVDEAMNSAGRYRVQGIPTLLLFKNGQVVEQIVGAVPKEQ
                     ITKALERHIA"
     gene            complement(7751..9325)
                     /gene="glgA"
                     /locus_tag="VGR03_16000"
     CDS             complement(7751..9325)
                     /gene="glgA"
                     /locus_tag="VGR03_16000"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV1995836.1"
                     /translation="MRILFVASEGLPFSKTGGLADVVEALPKALVAQGHEAAVVLPRY
                     RGTKATAVVMPSLTIPMGGTRLRFPAIADGTQLSGVRYYFVDDPEYFDRDGLYGGSSG
                     DYPDNPERYAEFCRAAIEVAKHVWPADVIHCQDWQTALVPVLLRTSYGDDPVMKDIPV
                     VFSIHNMGYHGQFTKDVLERVGIPQGVFHPGGLEFFGKVNLLKGGLVYSDYLTTVSRK
                     YAQEIQTPEYGCGLDGVARGRADRLVGILNGVDYTAWNPEKDKLIAVRYSAKDLSGKQ
                     VCKQELLELFGLSHDHLERPLIGIVSRFADQKGFDLIAEKAHELMREDLVLVVLGTGD
                     RKYEDLFRALAAAYPGRVGVKIAYDNTLAHKVEAGADMFLMPSRYEPSGLNQMYSLKY
                     GTVPIVRATGGLDDSIEPFDLEHGTGTGFKFKEYTGEALLFAVRQALHHYMDERIWKR
                     IQLNGMAKDFSWKTPAAEYAKLYEAARAARGLSPLAQSSGTAAQGSGQPSRGVGQSPQ
                     AAGQTARNQKPAGTSN"
BASE COUNT         2070 a         3510 c         2571 g         2232 t
ORIGIN      
        1 ccatccatct tgcccagcac gagtacatca acgccctcgg caccgactcc tggcgcaagg
       61 cggtcaacaa ttccgtccac caaaacttcg actaccgcaa cgattccgct gatcccgaac
      121 cccctccaac cccgctccaa gcgccatgcg catctctaac cccaacccct ttagaatcaa
      181 cacttataga aatacccgca agtgttgatt ctaaaccact tacgtgaact ctaagtcctt
      241 tagatgcaac acttatgaaa aacagggggg tgggggtatt gtcagagact ccgcagatca
      301 gtcgtgccgc gctccatgga gaactttcac cgcgtgcggc agcaggtggg ccactgcttc
      361 cagcgattcc accgcgcctt tggggcttcc cggcaagttg acgatgagcg tcttcgttcg
      421 caccgcagcc gtaccgcgcg agagcactgc cgtcggcgtt ttctccagcc ccttgcgccg
      481 catctcttcg gaaagtccag gcacaatccg gtcggccacc gcctgggtcg cttccggagt
      541 gttgtcccgc ggcccgatcc ccgttccgcc cgtggtcaag agaacgtcca cgcgcccgga
      601 atccgccacg gcgcgcaact gctcgcggat atttgcggga tcgtccgaga cgtgcaaagt
      661 cgaagtgact tcccagccca gcccgcggca gaatgccaca accgcggggc cggacaaatc
      721 ctcgcgctcg cctttcgtca cggagtcgct gatggtgagg accgctacgc gcatcaggaa
      781 ggtttcttcg ggagactcac ggcgtcccga ccgtatcgcg attggcctcg tccatgagcc
      841 gcatcgcttc catcagcaag ccggtcgtgt tgcgcgtcgt ggtcgtcttg gtcgaggcat
      901 tcgcggcgtt gaaatcgatt tcaaattctc ccgccgtcca taacacaact ttgtaaacgg
      961 cgtcgtcgcc ttccaccgaa ccaatcttcg catcgcggca ctgcccgctg gcaaaataca
     1021 gttcacactg cgctccgtcg tgttgcacga tcaaccggca agacttctgc cccatctcca
     1081 gcgactgcat caggtcgatc atgctcatct cttccagccg tccttgaatg acgcccggcc
     1141 ttgacgcctt cttctgcagc ttttcgaggt gcaggcgatc gacaactttt ttcgcgatac
     1201 gtaccaaatc tttaatcaaa aatggcttgg tgatgaaatc ctccacgcca tccaccagcg
     1261 gcctcagacg ctcttcgatg tccggccggc tggccatgaa caaaaacgga atgtgccgcg
     1321 tcgattcgcg cgcgcgcaat ttttcgaaga gttgcctgcc gtccaggccc ggcattttgt
     1381 aatcgcagag aatcacatcg ggcggtgcgt cgacgacttt caggagcgcg tccgcgccgt
     1441 ccgtggaagt ttccacttcg cagaacggct caagcccctt cacgatcaag tcgcgcacca
     1501 gcggattatc atctgccagg agcagcttta ccgttttgtg tcctgccgtg ctcatcgcgc
     1561 gcgcctccgc gcagccttcc gccgatggaa gtcaccgctc ttcccgccgg tcttctcggt
     1621 gagaacgatg tccgtgattt ccatcccttt atcgagggct ttgcacatgt cgtagactgt
     1681 gagggccgcg gcggcaacag cgaccagcgc ttccatttcc acgcccgtct gggcggccgt
     1741 ggtcactgtg gatcttactt ccacgccatt ttggcacagc cgtgcggtca catcaatgtg
     1801 cgtcaggggc agtggatgac aaagaggtat ccactccgca gtcttcttcg ccgccgcaat
     1861 tcccgcgatc cgcgccactt ccagcgggtt tcccttcggg tttttcaacc gccggactgc
     1921 gctcacaacg cgcggcttca tcttcacaaa accccgcgcc gctgccgttc gcgtcgtcac
     1981 cgctttttcc gacacgtcga ccatccttac ttcgcctttc tttccgtagt gcgaaagctt
     2041 tgccatatcc gcgcgcctag actacgtcct tcctcagtag caaggaaaca ctctcgccgg
     2101 tctcgatctt ttctcggtct gctggcacca ccaggaaaca gttcgccttc gctagcgccg
     2161 cgatatcgcc cgagccttgc catttcaacg ccttcacttc cggcaccgcg ccacgccact
     2221 ccactcgcgc gggcaaaaaa tgcgccagcc cgggcttctc gttcaatgct tccgctagcc
     2281 gcgcctcaac catcggcaac gcgcgcgcct ccgcaccgct caacaaatca atcgcgggcg
     2341 ccacaaacaa ctcaaacgtc accatcgtcg aaacgggatt ccccggcaat ccgaacaccg
     2401 gcttgccctc gcacattccg aacaccgccg gcttccccgg ccggatcgcc accgcatcga
     2461 agaaaaattc cgcgcccatg gctttgagca cgctttcaac aagatcgtac ttccccatcg
     2521 agacgccacc cgacaacacg agcgcatctt cctttagtcc gcgctcgatt ttctctccca
     2581 aatcctcgat gcggtccgcc gcatttccca ggactatggg ttcgccgccc gcgatgcgaa
     2641 cctgcgcagc gagtgagacg ctattactgt ttcgaatctg aaacgggcct ggcgtttcgt
     2701 cgatcaagac cacttcgtcc cccgtggaaa gaatggccac gcgcggccgc tgcgcgcatt
     2761 gcaagtgcac cgcgccgact tgcgccgcca gcgccagctc cgcgtatccg aggcgcatac
     2821 ctgggctcaa aattatctgc gcggcggccg cctcgcttcc ccttggcacg atgttttgcc
     2881 cgggctgcgc cgctcgttcg aatcgcacga ggtcgccttc acggctcgtg tgctcgatca
     2941 tcaccaccgc atccgcgcct gccggaaccg ccgcgccggt catgatttgc aggcaagttc
     3001 ccgcggcaag tgtttcgctc accgtgtcgc cagctttgat ttctcccaca cacttcagtc
     3061 ttgtgcctgc cgcagcttct ttcgaacgca gcacgtaccc atcgcgcgtc gagcggtcaa
     3121 acggcggata ttcacggtcc gtctgaactt cttgcgccag caccagtccc agcgcgtccc
     3181 acacgctcac aaccgcggtc gcgcgtggtc ctttcctctt cgcaacttgt tcgatgactt
     3241 tgttccgggc ttgctcgtag gtgagcatga gtgaggtctt gtagcgccgc gcttcagaac
     3301 ggcatatttt ggttctgtgc tttcgttctt ctctctgcgt tctctgcttc tctccgttta
     3361 gtatctcttt acagccaccg cgccagcagc gtccccgcgg gcacaatcgt cgcgtcgcgc
     3421 tcgggtccgg tcgaaatcat tccaatctcc acctgcagaa aatcggaaat aaagcgcaga
     3481 tagtcgatcg ccgcttttgg gagtttcgcg ccatctttca cgccgtacgt ggtttcattc
     3541 cagccgggca gtgttttgta ttccggggtg ccgtgttcgt attcttccgc gagcgccggc
     3601 atttccttca agggcgtgcc tttgtatttg tagccgacgc acacttgaat ttctcgctgc
     3661 gtatcaaaaa cgtccagttt ggtgatcacc atagaatcga tgccgttgat catcttcgcg
     3721 taacgcagca cctgtaaatc cagccagccg cagcgccgtg gacgccccgt cactgcgcca
     3781 aattcttttc cgcgttcgcg cacttctttg gcatcgagat cgggcatctc cgtcggaaac
     3841 ggtcccccac ccactctggt agtgtaagct ttcgaaattc caagcacgtg cttgattttc
     3901 gtcggcggca cacccacgcc cgtcgccgca ccgcccgagg tcgcgctcga agaggttacg
     3961 tagggatacg ttccatggtc gatatccagc atggtcccct gtgcgccttc aaatagcacg
     4021 gacttcccct catccagtgc acggttcaac agttccgccg tatcgcaaat gtacccgcga
     4081 atcttttcgc ccagcttcag cgtctcttcc acctcatgcg aaaagtcgat gtccgcgccg
     4141 tacgcgccct tcgccagccg gttcttctcc gcgaccacgt gcgcggcctt ctttcgaaac
     4201 agctccgggt ccagcaggtc gcacatgcgc agcccgcgcc gcgccatttt gtcttcgtag
     4261 gccgggccaa tcccccggga agtcgtgcca atcttgttcg cgccgcgagc gccttccgca
     4321 gccttgtcca gctcccggtg atacggaaaa atcaagtgcg cccgattgct gacgtgcagc
     4381 cgccccttta cgtctatgcc tgccttggag agcatcgcaa tttccgccac cagcgccgcc
     4441 ggatccacca ctgtgcccgt tccgattacc gcatgctgct tcggccgaag tatgccgcac
     4501 ggtatcagtt gcagcacgta gcggtctttc ccgatgatga ccgtgtgccc cgcgttgtgg
     4561 ccgcccgcga cgcgtgcaat gtagtcaaac gatcctgcaa ggtagtccac caccttgcct
     4621 ttcccttcgt cgccccactg cgcgcccacc acgacgaccg atttcatgca ggctttctcc
     4681 tcaaggtagg acaggtattc ttgcctgcca tgccctcttc tcaaccacac ttcagccgct
     4741 aaacgttcac atcactttgc tgctttggaa agcaccacga aacggcaggt tcttatcgta
     4801 agctcgcccc gctctcaacg caagcaagcc ttgcgctcac tcctatttcg acaaatctcg
     4861 atgccggaaa caagtcacaa ggtgatcgtt caccatcccc gtcgcctgca tcagggcata
     4921 acaaatcgtc gagcccacaa aacggaatcc ccgcttctgc aaatccttgc ttagcgcgtc
     4981 agattccgcc gtctttgctg ggacttgctt gtgattcttc cacgcatttt gcttcgcctt
     5041 tccatcgaca aaccgccaaa tatatgcatc gaaggccgcg tactcttcct gcactttcaa
     5101 gaaggcgcgg gcgttgccga tggcagcgcc gattttgagg cgattgcgga tgatcccggc
     5161 gtcgcgcagc agttcgcgga ccttcttctt ttcgtagcgg gcgactttga cgggatcgaa
     5221 agcgtcaaag acttcgcgat accgcccgcg tttgcggaga atcgtgtccc agctcaatcc
     5281 cgcctgtgcg ccttccagta tcaagaactc aaaccatttt tgctcgtcat gcacgggcac
     5341 gccccattcc tcgtcatgat acgggatgtt cagctcgttc ttcgcccagg cacaacgcac
     5401 tctggtgatc ttgtggcttg ctgtcatggc cgttgtggtt tcactcccgg caaattcttc
     5461 acttcttctc cggcaccgcg ataagccacg gctgctgcca gataaaatga tgcgttcccg
     5521 gaatctgccg ccaaccgtcc gcgggatggg cggcatctgc attctttgta acgacggctc
     5581 ttgctccaac ggaggaaaac agcgcgataa cttgctgcct tcgcgccgca tccgcagtga
     5641 cgaaacgcgg ttgttcgatg tccgggatct ccacgatgat tcgcacgccc gcgagatgcg
     5701 cccagtaagc gtccaagccg gtcccgacat acgcgacgtc cgttcctggc agaatcccca
     5761 tggcgttaag ctgttgcgca acgacccacg gctcataggg cttgttgcgt atcacatcat
     5821 atagatcgcg cgcgacgggc cacgccacgg caagcgccgg agcaagaatc acgacgagat
     5881 gaaagcgctt cagcaattgc ggctccgccc ccgtgacaat tctcattctc gccagcatcc
     5941 acagcatcag catcagcgcg aacggcgcga cgtagcgaaa ttcgaccagc acgagcgagt
     6001 acatggcaag cgcggcaacc gaaggcaaaa ccaccagcca cgcgcccgaa gaagagcttc
     6061 cccacgcgag tgcccttctc ccgacgaccc acaccgccac caacaccacc cagagcgcgc
     6121 cgctctttga aaatatcttg aaatacaaat tggccgcccg aaacagcgcc cacaattgcc
     6181 acttcagcag gaagtgcggc cgcactcctt cataccaata cgatgggtca taccatggcg
     6241 ggtatgagcc ctgtatcgga gaggcaaatt caaacaccgc cggctgggag aaaattctcc
     6301 gggtcggatg ggcggggttt cccgtcccca atggctctcc ctgccaatga accgttttcg
     6361 ttgcgcgatc gatatattcc gtgtagctga tgcgtgcgga atcgccaaac gtcagtcgtt
     6421 ccttcgcagc cgagagcgcc agcacaagcg gcagcgcgaa gccggcaaac accgcagtcg
     6481 ccagcagcgt gcgaagagcc gcgccgcgaa aagaagctcc cgcgatgcgg cacaggcaga
     6541 aagcgcagga cagaaaaacg aaggacaacg gaaacatcgc cgttttcgct aggtaaccga
     6601 gaccgagcaa tgcgccaaat cccacgaaca cgagccaact gccccggccg cgccagatgc
     6661 gaaacagcag cgccgtagca agatacacaa gcgcggcaac gcaaaggtcc ggcgtcaccc
     6721 atacggggcc cagccaaaag taacttgccc agagaaaaaa gacgtatccc cagatccaca
     6781 cagttcgcgg ggaaacaggc aaagatttct cggttgattc tccggaagat tgccgctgca
     6841 ggatcaattc tttcagaaat agttcgaacg aggcgaggct cgcgagatac accgcaaaat
     6901 tcaggagatg cgcagccgta aattcccact gcattggcgg atggaagtac cgaaaaacga
     6961 gactcaacag aaaaggatag agcggactcc agtaggcgtt gacaatctgg tgcaagccaa
     7021 agcgcgccgt ggcccaggcg atttcgatat aagaaatgct gtcgggggac acgtcattgc
     7081 ggcgtgccca cacgttcaac agccccaaaa ccaggatcac gcaccaaaag aagattcgag
     7141 gatgaggctc aagcagcgca cctgccggag aaagtggatc acgctgttcc gcctgcgagc
     7201 gtcgcagttg atgcactctt cctccgccga aaaaaatcaa ccgaagctgg cgagaggatt
     7261 atgtcaggac ttttgcccgc agcggtaggt gaaacgacag aggcccgcga aaaccggggc
     7321 gccccgatcg tgacgccccg caattgccgt caaatttacg cgatgtgccg ctccagcgcc
     7381 ttggtgatct gctctttcgg cactgctccc acgatctgct cgaccacctg tccattcttg
     7441 aacagcagca acgtggggat tccctgaacg cgatagcggc ccgctgaatt catcgcttca
     7501 tcgacattca acttcatcac tttgagcttg cccgcatagt cgtgggcgat ctccgccacc
     7561 gtcggcgcga gcatgtggca cggacggcac cattccgccc agaagtccac catcaccggc
     7621 tgcgaattgc tggccgttaa aacctcggct tcgaaatttc catcagttgc tgcctgaatc
     7681 gaattgtctg ccatattttt tatcctcagc ggcctaccca tctggccgcc gctctctctc
     7741 ctcaatcgat ttaattagat gttccagcag gcttttggtt tcgtgcggtt tgccccgcag
     7801 cctgcgggct ctgccccacc ccgcggctcg gttgtcctga gccctgagcg gccgttcctg
     7861 aactttgcgc gagtggggac aatccgcgag cggctcgcgc ggcttcgtac agcttcgcat
     7921 actcagccgc aggcgtcttc cacgaaaaat ccttggccat cccgttcaac tggatgcgct
     7981 tccagattcg ctcatccatg tagtgatgga gcgcctgccg caccgcaaac aagagcgcct
     8041 cgccggtata ctccttgaac ttgaacccgg tccctgtgcc gtgttccaga tcgaacggct
     8101 cgatgctgtc gtccaacccg ccggtggctc tcacaatcgg caccgtacca tacttcaagc
     8161 tatacatctg attcaacccc gagggttcgt agcgcgaagg catcaggaac atatccgccc
     8221 ccgcctcgac tttgtgcgcc agcgtgttgt cgtacgcgat cttcacgccc acgcgccccg
     8281 gatacgctgc agccaaagca cggaacaaat cttcatactt gcggtcgccc gttcccaaaa
     8341 ccaccagcac caggtcttcg cgcatcaact catgcgcttt ctccgcaatc aaatcgaatc
     8401 ccttttggtc cgcgaagcgc gacacgattc cgatcagcgg ccgttccaga tgatcgtgtg
     8461 acaatccaaa caattccagc agctcctgct tgcacacctg cttcccgctc aaatcctttg
     8521 ctgagtacct gaccgcgatc agcttgtcct tctccggatt ccacgccgtg tagtccacgc
     8581 cgtttaagat ccccaccagc cggtcggctc ggcctcgcgc aacgccatcc agcccgcatc
     8641 cgtattccgg cgtctggatt tcctgcgcgt atttcctgct gacggtcgtc aaataatccg
     8701 aataaaccaa tccacccttc agcaggttca ctttcccaaa aaactccagt ccgcccggat
     8761 gaaatactcc ttgcggaatt cccacgcgct ccaacacatc tttggtgaac tgtccgtgat
     8821 accccatgtt gtgaatggag aaaacgacgg gaatatcttt catcaccgga tcatcaccgt
     8881 aagacgtccg cagcagcacg ggcaccagcg ccgtctgcca atcctgacaa tgaatcacat
     8941 ccgccggcca tacatgcttc gccacttcaa tcgccgcgcg gcagaactcc gcgtaccgct
     9001 ccggattgtc cggatagtcc ccgctgctgc cgccatacaa tccgtcgcga tcgaaatatt
     9061 ccggatcatc cacaaagtag tagcgcacgc cactcagctg tgtgccatcg gcgatcgccg
     9121 gaaatcgcag ccgcgtcccg cccatcggaa tcgtcaagct cggcatcacc accgccgtcg
     9181 ccttcgtccc gcggtaccgc ggcaacacca ccgccgcctc gtggccctgc gccacgagcg
     9241 ctttcggcag cgcctccacc acgtccgcta ggccgcccgt cttcgaaaac ggcagcccct
     9301 ccgatgccac aaacagaatt ctcatgattt gctcatcgcc tgcatcataa ccattttacg
     9361 ccgcggcctg ccaaaaacct aagtgtgatg cgagccgcct tccaaagccg gcatttgcct
     9421 ttatagggcc gccttccgag gcgggccacc ccgtggtgcc gcggcactcg tccctcctac
     9481 ccgaccctgt cggggagcac ttcgggcacc tcttccagag aactctccta acgaattcct
     9541 cataaacctc tcaggacttc ttagcgtttc cgctcctact ctttccttgt ccgggctagg
     9601 cattcacgcc agtccgagtg aggcctctat gtcgcttacg gtatttctgg cgttttgcat
     9661 tcttggatgt gacttcttgc tctatgtcct gtttcagtgg acatacggtg agaagcacag
     9721 aaaacacgct cgcttgtcgg acgcgcgccg cgagagacgc cgcaccctct ccggccaaga
     9781 agtcggagag gctggagtaa tctcgtttcc gcagcgctcg atgcattacc gaaccggctt
     9841 cttctgatcg cgcgctgcgt cctccacccc cgtcatttag aacggagcag gccggcgctt
     9901 tttcttcccg cttcgctcct gctcgctctt ctccatcccg catgcttttg cgggatgaac
     9961 cggtcggcct gcgcagagag aaatctctct tctctttcat ctccccgcgt catccgccat
    10021 atgttcgggg ccgaacgaag agtctttgcc cagcactttt gctttggaaa gtgatgccca
    10081 atgactcgcg cgaggcctct ctttctcttg atatccacgt gttccgtctt tcgtagcgcc
    10141 cgcatccttg ccagcatctt tgctttcctc ccaccgtcac tgcagcgaag gccgctgctc
    10201 gcaaattgag agccatttct cctccagctt cccttctccc acccgaatcc atttggcgca
    10261 tcgcgcccgg gccaccgcca aagttgttcc aaagtaaaca gccatccgtc cccgccgcct
    10321 ttagcatgct gcatgggaat agttttccag cgtgcacggc ggaaccatca ctgtgcgtga
    10381 caa
//
LOCUS       DASYVO010000471         5864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_208626, whole genome shotgun sequence.
ACCESSION   DASYVO010000471 DASYVO010000000
VERSION     DASYVO010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208626"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /gene="bshA"
                     /locus_tag="VGR03_16005"
     CDS             complement(<1..872)
                     /gene="bshA"
                     /locus_tag="VGR03_16005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HEV1995837.1"
                     /translation="MRIGITCYPTYGGSGVVATELGMELAARGHDIHFISYAPPIRMN
                     RDGERIHFHEVEVVSYPLFDHPPYTLALATKMLEVFESESLDLLHVHYAIPHSVSALL
                     ARSMAAPRRLPFVTTLHGTDITLVGSNRSYLPITRFSIEQSDGVTAISRYLLNQTIKE
                     FDIKRPIEVIPNFVNCDLYNRTADKGFAAQWAPNGEPILMHLSNFRPVKRLPDVVEIF
                     ALVRAKMPAKLVLIGDGPDRGAAEYLVRKHCLQKDVFFLGKQDNVYEKLAAADLFLLP
                     SQLESFGLAALEAMA"
     gene            1040..1804
                     /locus_tag="VGR03_16010"
     CDS             1040..1804
                     /locus_tag="VGR03_16010"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1995838.1"
                     /translation="MTRLSGKVALITGGGTGIGRAIALAFAREGASVAVAGRRLEKLR
                     EVIVQIQKQGGAGLAMECDVTRAREVERAVKGTVERFGRLNVLVNNAGTLHVSTVEGI
                     SEEEWDRVMTVNVKGPFLVSRAVLPEFRKCGGGAIVNIGSVLGLFAVKDRAAYCVSKG
                     GVTMLTKAMALDHAHEKIRVNCICPSVVETDLVKGVFNETEHGQALLKARLATIPLGR
                     LGRPEDVAEMAVFLASEESSWLTGAAIPLDGGVTAY"
     gene            1823..2269
                     /locus_tag="VGR03_16015"
     CDS             1823..2269
                     /locus_tag="VGR03_16015"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV1995839.1"
                     /translation="MTKRMSKEEKRVTELVRRRMKESRSTELLGFDVESVHAGRAIFR
                     LDVRPNHKQIHGVVHGGILAALADTTAAIAAYTAIPKGTEIATVELSINYLEPVPGGR
                     IKADARVLRAGRNFVVTECEIFNEDGSLAAKALLTFGATAGHSLHK"
     gene            complement(2275..2610)
                     /locus_tag="VGR03_16020"
     CDS             complement(2275..2610)
                     /locus_tag="VGR03_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995840.1"
                     /translation="MVNRRLFRPGFPDVQEPSAPRPQHIGPIPSKKPAPPETTHAENF
                     YWVKQMQSHTPMAVVLEDGEVLRGTVEWYDRDCIKLTRQGSPNLLIFKRVIKYVHKDG
                     DESAQGGDE"
     gene            complement(2726..3853)
                     /locus_tag="VGR03_16025"
     CDS             complement(2726..3853)
                     /locus_tag="VGR03_16025"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995841.1"
                     /translation="MALSEFLGNERIVTALRGALRSKRVPHTLLFTGARGVGKFTLAR
                     MFAQAANCERLPDDFCGKCATCQRISQLADLQTLLEQGLVERGESADAATVERVPLIL
                     QSHPDVWALVPDPVRLKSPVARPMLRVGQLRAVQRAAYFQPMGRRRVFILDGAETMRW
                     DVANVFLKILEEPPGSATLILTAPSPYALLPTIVSRCLQFHFAPLPQAAVEQILKEKT
                     HRKPAEIKLAAQLAEGSPGLAIEMDVDAASQRRRSALRILERAAQGQGFAQLFSDTSA
                     LAKDRDSSFEDQIGVFYGLLTDLLELTSKLKEPVLRNAPLAKELESLGKSVDSAWVLR
                     AIAGFDDLYVGARRNLNRQLGLDALTASLAPANSRPAALRP"
     gene            complement(3857..4561)
                     /locus_tag="VGR03_16030"
     CDS             complement(3857..4561)
                     /locus_tag="VGR03_16030"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995842.1"
                     /translation="MTAPGKLIAVEGIDGSGKRTQVELLTLTLKARGHSVYSTGFPQY
                     DSWFGKMVGQFLNGDLGSLEIVDPHFTAMLYAGDRFEAKPKLESALNNGQIVLVDRYI
                     GSNLAHQTARVPPEKRSEFLEWIEHLEYRVYGLPRENLILYLRVPPREAQRLVERKSA
                     RSYTSARKDLLEASLRHLDDAAEMYDSLSRSAPWATIQCFDTTLNAIREPGEIATDVL
                     AAVEGVLSSASSGEER"
     gene            4659..5363
                     /locus_tag="VGR03_16035"
     CDS             4659..5363
                     /locus_tag="VGR03_16035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV1995843.1"
                     /translation="MAQTSVRENLLRIQEMIASAAARVGRRAEEITLIGVSKTHPASA
                     IRGAFEAGIRHFGENRVQEWEGKRAGTEGLAATWHLIGHLQSNKTARATRLFHSIDSV
                     DDFAIAQRLDRARAEAGITGKLRVLIEVSVAPEETKSGVEISELAALAEGFADLPRLE
                     LAGLMCIPPYLEEVERVRPYFRRLRELRDELAKNLNLALPVLSMGMSHDFELAIEEGA
                     TEVRVGTALFGTRDVK"
     gene            5382..5675
                     /locus_tag="VGR03_16040"
     CDS             5382..5675
                     /locus_tag="VGR03_16040"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEV1995844.1"
                     /translation="MLEVQEREGAVTFLVRVQPRASKDEIAGEIGGALKVRLRAPAVE
                     DRANEALVEFLAELLKTSRSAVRILSGERSRTKRLEIRGVTRQQILALLVQEA"
BASE COUNT         1201 a         1414 c         2121 g         1128 t
ORIGIN      
        1 gccatggctt cgagggcggc gaggccgaag gattcgagtt gcgaaggaag aaggaacaga
       61 tcggcagcgg cgagcttttc gtagacattg tcctgtttgc cgaggaagaa aacgtctttc
      121 tggaggcagt gcttgcggac gaggtattcg gcggcgccac ggtccgggcc gtcgccgatg
      181 aggacgagct tggcgggcat cttggcgcgg acgagggcaa agatttcgac cacatcgggg
      241 aggcgcttga cggggcggaa attcgagagg tgcatgagga tgggttcgcc gttgggagcc
      301 cattgggccg caaaaccttt gtcggcggtg cgattgtaga gatcgcagtt cacgaaattg
      361 ggaatgactt cgatggggcg cttgatatcg aactctttga tcgtttgatt gagcaagtag
      421 cgggagatgg cggtgacgcc gtcgctctgc tcgatcgaaa agcgcgtgat ggggagatag
      481 ctgcggttgc tgccgaccag cgtaatgtcc gtgccgtgaa gagtggtgac gaaagggagc
      541 cgccgcggcg cggccatgga gcgcgcgaga agggcgctga cggaatgggg aatggcgtag
      601 tggacgtgga gcaggtcgag cgattcggat tcgaaaactt cgagcatttt tgtggcgagg
      661 gcaagggtgt acggcggatg gtcgaagagc gggtaggaga ctacttccac ttcgtggaaa
      721 tggatgcgct ctccatcgcg attcatgcgg atcggcgggg catagctgat gaaatgaatg
      781 tcgtggccgc gggcggcgag ttccatgccg agctcggtgg cgacgacgcc ggagccgccg
      841 taggtgggat aacaggtgat gccgatgcgc ataagatgtg ctttcgagtc tccgtcgcgg
      901 tattgggagc cgctaagata caacacttcg ctgccggagc ggcttgcagg gcagggagct
      961 gaatgctaga gtcgcgccca ccgtgtgagg caccaggcgg cggtgtgtgc ctctattaga
     1021 cgcggggaca gggaaatccg tgacacgact ttctggcaag gtggcgctga ttacaggtgg
     1081 cggcacgggg atcggtcgag cgatcgcgct ggcgtttgcg cgggaaggcg cgagcgtggc
     1141 cgtggcggga agacggctgg aaaagctgcg agaggtgatt gtccaaattc agaagcaggg
     1201 cggcgcggga ttggcgatgg agtgcgatgt gacgcgggcg cgggaggtcg aacgggcggt
     1261 gaagggaacc gtggagcgat ttgggcggct gaatgtgttg gtgaacaatg cggggacgct
     1321 gcatgtctcg acggtggagg ggatcagcga ggaagagtgg gaccgggtga tgacggtcaa
     1381 tgtaaagggg ccgtttctgg tgtcgcgggc ggtgctgccg gagtttcgga aatgcggcgg
     1441 gggagccatc gtgaatattg gctcggtgtt ggggctgttc gcagtgaagg atcgggcggc
     1501 atattgcgtg tcgaagggcg gcgtgaccat gttgacgaag gccatggcgc tcgatcacgc
     1561 gcatgagaag atccgagtga attgcatttg cccatcggtc gtggaaacgg atttggtgaa
     1621 gggcgtattc aacgaaacgg aacacggaca ggcgttgctg aaggctcggc tggcgacgat
     1681 tccgctgggc cgattggggc ggcccgaaga tgtcgcggag atggcggtgt ttctggcgtc
     1741 ggaagaatcg tcgtggttga cgggggccgc gattccgttg gatggagggg tgaccgctta
     1801 ctgacggggg cggcgaaacg cgatgacgaa gagaatgtca aaggaagaga aacgggtcac
     1861 ggaacttgta cggcggcgga tgaaagaaag ccgctcgacg gagcttctgg gattcgacgt
     1921 ggaaagcgtg cacgcgggac gggcgatttt ccggttggac gtgaggccga accacaagca
     1981 gattcatggc gtggtacacg gagggattct ggcggcgctg gcggatacca cggcggcgat
     2041 cgcggcctat acggcgatac ccaaggggac ggagattgct accgtggagc tgagcatcaa
     2101 ctatcttgag ccggtgccgg gcgggcgtat caaggcagat gcgcgggtgt tgcgcgcggg
     2161 cagaaatttt gttgtgaccg agtgcgaaat tttcaacgag gacgggtcgc tggccgcaaa
     2221 ggcgttgctc accttcggag caaccgccgg gcattcactt cacaagtaga gagcttactc
     2281 gtcgccgccc tgggccgatt catctccatc cttgtggacg tacttgatca cgcgcttgaa
     2341 gatgagcagg ttggggctgc cctggcgggt gagcttgatg cagtcgcgat cgtaccattc
     2401 gacggtgccg cgaaggactt caccatcctc gagaacgacg gccatcggcg tatgggactg
     2461 catctgcttg acccagtaga agttctcggc gtgagtcgtc tccggcggcg cgggcttttt
     2521 actggggata gggccgatat gctgagggcg gggtgcgctc ggctcctgga cgtccgggaa
     2581 gcccggacga aataaacggc ggttcaccat gactgctcct tctaacctgc ggggtggaat
     2641 atctgctctc tatgatgcgc acgggagcct gtgggttaca gtctagccat ttaattttgc
     2701 aaaggcgaat atttgtcagc gccgatcagg gacggagcgc ggcgggccgc gaatttgcgg
     2761 gagcgaggga tgccgtcagg gcgtcgaggc cgagttggcg gttcaaattg cggcgcgccc
     2821 cgacgtaaag atcgtcaaaa ccggcgatgg cgcggagcac ccacgcagag tccacggatt
     2881 tgccgagcga ttccagttct ttcgcgagcg gagcattgcg caagacgggt tctttcaatt
     2941 tggacgtaag ttcaaggaga tcggtgagca aaccgtagaa aacgccgatt tgatcctcga
     3001 aggaggaatc gcggtctttc gcgagagccg aggtgtcaga gaacagttgg gcgaatcctt
     3061 ggccttgcgc ggcacgttcg aggatgcgca gtgcgcttct gcggcgctgg gaagctgcgt
     3121 cgacatccat ttcgatggcc aggcccgggc tgccctccgc gagttgcgcg gcgagtttga
     3181 tttccgccgg cttgcgatgg gtcttttctt tcaagatttg ttccaccgcc gcctgcggca
     3241 gcggcgcgaa atgaaattgc aggcagcgcg agacgatggt aggcagaagc gcataaggcg
     3301 agggcgcggt aaggatgagc gtcgccgacc cgggaggctc ctcgagaatt ttcaggaaga
     3361 cgttggcgac atcccagcgc atggtctccg cgccatcgag gatgaagacg cggcgccggc
     3421 ccatgggttg aaaataggcg gcgcgctgga cggcgcgcaa ctggcctaca cgcaacatgg
     3481 ggcgcgctac cgggctcttc agtcggacag gatcgggcac gagcgcccac acgtccgggt
     3541 gcgactgcag gatcagaggc acgcgctcga cggtggcggc atcggcgctt tcgccgcgct
     3601 cgacgagacc ttgttcgaga agcgtttgca gatcggcgag ctgggaaatg cgttgacacg
     3661 tggcgcactt gccacagaag tcgtccggga ggcgctcgca attcgcggcc tgcgcgaaca
     3721 tgcgcgcgag agtgaatttg ccaacgccgc gcgcgccggt gaagagcagc gtatggggga
     3781 cacgtttgct gcggagagcg ccgcggagcg ccgtgacgat acgttcgttg ccgagaaatt
     3841 cagaaagagc catgcgctag cgctcctcac ccgaggaagc cgaggaaaga actccctcga
     3901 cagcggcgag aacgtcggtg gcaatctctc ccggttcgcg aatggcgttg agcgtggtat
     3961 cgaaacactg gatggtcgcc cagggagcgc tacgggaaag cgagtcgtac atttcggcgg
     4021 cgtcatcgag gtgacgaaga ctggcctcga gcaaatcttt ccgggcggag gtgtagctgc
     4081 gcgccgattt acgttcgacc agccgctgag cttctcgcgg cgggacgcga aggtagagga
     4141 taaggttttc gcgggggagg ccgtagacgc ggtactcgag atgctcgatc cattccagga
     4201 attcggagcg cttttctggc ggaacgcgag cggtctggtg cgcgaggttg gaaccgatgt
     4261 agcgatccac aagaacaatt tggccgttgt ttagggcgga ttcgagttta ggcttggctt
     4321 caaagcggtc gccggcatag agcatggcgg tgaagtgagg gtcgacgatt tcgagcgagc
     4381 ctagatcgcc attcaggaat tggccgacca ttttgccgaa ccaggaatca tattgaggaa
     4441 agccggtgga atagacggaa tgcccgcgag ccttgagggt aagtgtcagg agttcgactt
     4501 gcgtgcgctt gccactgcca tcgatgcctt cgacggcgat gagttttccg ggagcggtca
     4561 ttttccgagt gaccgctgat ccatcagcgg ccaaaagcca gtctaaccgc agagactctg
     4621 ctctgcaatg ccgcgcgtgc tacgattcga attcgaacat ggcgcaaact tcggtgcggg
     4681 aaaatctgct aagaattcaa gagatgatcg ccagcgcagc ggcgcgcgtg ggccggcgcg
     4741 cggaggagat cactttgatc ggggtgtcga agacccatcc ggcgagcgcc atccgcggag
     4801 cgtttgaagc agggattcgg cactttggcg aaaatcgcgt ccaggagtgg gaagggaagc
     4861 gcgccggaac ggaagggttg gcggcgacct ggcacttgat cggtcatttg cagagcaata
     4921 agacggcgcg ggcaacgaga ctttttcaca gcatagactc cgtcgatgat tttgcgatcg
     4981 cgcagcggct ggaccgcgcc cgtgccgagg caggtatcac aggcaaactg cgggtattga
     5041 ttgaagtgag cgttgctcca gaggagacga aaagcggggt ggaaataagc gagcttgcgg
     5101 cattggcgga agggtttgcc gatctgcctc gattggagct tgccggactg atgtgcatcc
     5161 cgccgtacct ggaggaagtg gagcgggtgc ggccctattt ccggcgattg agagagcttc
     5221 gtgatgaatt ggcgaaaaat ctcaatctcg ccttgcccgt tctgtccatg ggaatgtcgc
     5281 atgattttga actcgcgatc gaagagggtg cgacagaagt gcgtgtgggt acggcgctgt
     5341 ttgggactcg ggacgtcaaa taagggatcg tgaggagtcg gatgctggag gtccaagagc
     5401 gggaaggcgc cgtgactttt cttgtgcgcg tgcaaccgcg agcgagcaag gacgaaattg
     5461 cgggggaaat aggcggtgcg ttaaaggtgc gactgcgtgc gccggcggtt gaggaccggg
     5521 cgaacgaagc gctggtggaa ttcctggcgg agcttttgaa aacatccaga tcggctgtta
     5581 gaattctgag cggcgaaaga agccgcacga aacgcctgga aatccgcggt gtgacccggc
     5641 agcagatcct cgcgctgttg gtgcaagaag cttgacagtt gaaggttgtc cgagcgatgg
     5701 agctgattcc ccgatgagcg cagatattca aggaatccag agtgacttgc gggccgccaa
     5761 gatcgatggc tggctcttct atgattttcg cggacgcgat cccatcgcgc agcacatcct
     5821 gagtctgccg gaaggaatga ggacgagaag gtggttctat tttg
//
LOCUS       DASYVO010000472         7886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_68080, whole genome shotgun sequence.
ACCESSION   DASYVO010000472 DASYVO010000000
VERSION     DASYVO010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7886
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68080"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /gene="zwf"
                     /locus_tag="VGR03_16045"
     CDS             complement(<1..1024)
                     /gene="zwf"
                     /locus_tag="VGR03_16045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV1995845.1"
                     /translation="MAGDMGRSGSVYGSVVEPCTVVLFGASGDLAKRKVIPAMYDLAI
                     HNALGPRYTIVGFARTAMTEDAFRTMLGDAAKSISEVGPIDPKQWDEFAANLHYCPGD
                     YADQEAYAKLARRLAELESAKNIGGNRLFYLSTPPEVYPNIVEQLGRAGLSRPSSPNS
                     WVRIIIEKPFGRDLASAKTLNQIVLNVFDEKQVYRIDHYLGKDTVQNLLVLRFGNGIF
                     EPLWNRNYVDHVQITASETLGVERRGGFYETTGALRDMIQSHVLQLTSLVAVEPPASF
                     DANAVRNEKLKVLQSIRPFDLEMVAQAVVRGQYAPGKLGDKPMPGYREEPNVNPASKT
                     ETFVAGK"
     gene            complement(1081..1692)
                     /locus_tag="VGR03_16050"
     CDS             complement(1081..1692)
                     /locus_tag="VGR03_16050"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEV1995846.1"
                     /translation="MAEITTLFWDIGGVILTNGWDRGSRKEAANAFHLDWEEFQDRHD
                     LSIPAFDSGQITLNEYLDRTLFYRQRGFTREEFTAFMFAQSKEYPETRAILDKVASSG
                     KYFVGAINNEPLELNQYRIEAFDLRRNFLVFFSSCYVRSRKPEETIFRVALEVTQRPP
                     QQCLFIDDRPLNLECPRRLGMNTIHHQNADQLRSELGKHGVEV"
     gene            complement(1771..3510)
                     /locus_tag="VGR03_16055"
     CDS             complement(1771..3510)
                     /locus_tag="VGR03_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995847.1"
                     /translation="MPLPTQAIFPGLLESAYRRELQNLSANNVLARLWAKDASLWPAE
                     EFQAASLKSNLRWLDLPGQLGPLMARVAARAAMIEPAGFEDVVFITMGDSNLAAKSIL
                     RLPDAKLGKRTFLLENIDPDSVRAFDEWLRLDRTLFIFANKSGKHLETHSLLLYFLER
                     LRIQGIDSPARHFVTVTEENSYLGELTGEYDFLDSFLDPPGIHGRYSSLIHFNFFLAA
                     LCRLDPSDLLARTQAMRDACGPAAPQEANPAVSLGAFLAAAEIEGLDRLVFFSPDSLK
                     PVARRIGYLVGASTGKKGRGIIPIFGSPSYEPAMLQQGCCAAILRMAGEEAPELGRRY
                     DELREAGVPIVTIELNGPEELAVELFKWEIATALTCSQLGVDPFHDPDIRESRTRTVQ
                     ILEQIATKRQSPVPTVRVREGEVELYAEAETRRQISTLNMAEALRTFFGLRHREGYIA
                     LLPFMNFGEVQKAVFRRIRERLESMLGLPVLVTPGPRYIHAIGQVYLGGPAKGLFLLL
                     TAAPEKDLAIPGADYSFWQLQLALALGEFESLGRRRRPVIRLHLTGGAEPGLAQLETI
                     LNNALGKRRIVAP"
     gene            3712..4044
                     /locus_tag="VGR03_16060"
     CDS             3712..4044
                     /locus_tag="VGR03_16060"
                     /inference="COORDINATES: protein motif:HMM:NF003817.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEV1995848.1"
                     /translation="MANESCGPTPAPSEAVQPGARMVHCVKFDKELPGLDRIPWRGEI
                     GKRVYESVSKDAWKLWLEHSKMVMNEYRLNPLDPNSQKIMEEQMEQFFFGEGAKLPEG
                     YVAPKAKS"
     gene            complement(4041..5354)
                     /locus_tag="VGR03_16065"
     CDS             complement(4041..5354)
                     /locus_tag="VGR03_16065"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1995849.1"
                     /translation="MTNPSKRLLLFTAKLGYQTRSFEDAAHKLGVQLVYVTDRCHQLE
                     DPWGDRAIAVHFESPEVAAYTVMEALRGQDVSGILALGDRPAAAAAYAARGLGIRYNH
                     PAAVEACRNKLRMKEVFRDAGLRVPWFRNLAIDPTPEPVLLGISYPCVLKPLSLSASQ
                     GVVRANNRAEFIAAAARVRRLLKSPEILATREANLEQMLVEGYIPGREVAIEGLLTDG
                     ALSVLAIFDKPVPLEGPYFEETIYVTPSRLPDAEQRAIEQCARDAVRALGLSHGPVHA
                     ELRINEDGVWPLEVAPRPIGGLCARALRFSLGGASEPIGLEELLVRHALELPGWNLPC
                     ERIASGVMMIPVPESGILEGVSGEEAARSVPGITELAITARLHDAIAAWPEGASYLGF
                     LFARGSAPEKVEQALREAHEKLSFTITPVLPVEHPATRRMTNQRN"
     gene            complement(5379..6833)
                     /locus_tag="VGR03_16070"
     CDS             complement(5379..6833)
                     /locus_tag="VGR03_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_223146968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HEV1995850.1"
                     /translation="MNIVLISTYELGRLPFGLASPAAWLRKRGHNVVALDLARQSLDE
                     TAIGDAGLIAIYLPMHTATRLAAQLIPALRKQNPSAHLCCYGLYAPMNVEYLRALGVS
                     TILGGEFEEGLVQLAERLATNGKQAIDQPEESLISMARLQFEVPDRSGMPAMEKYAHL
                     IVPGDGYRIVGSTEASRGCKHLCRHCPIVPVYKGVFRIVGRDVVLEDVRRQVAAGAQH
                     ITFGDPDFFNGIRHAMELAEAFHQEFPAITYDVTIKIEHLLKYENHLRALRDTGCLFV
                     ISAVESVDDAVLGFLDKGHTRADFLRVIETFRELGMTLHPTFVPFTPWTTVDGYLDLL
                     QVIEEQGVIENVAPIQLGIRLLIPEGSRMLELEEVRRLVGPFDPQSLAYPWKNSDPRL
                     DALSETVQEIAAAAERQRESRPATFERIWKAAHAAAGLPSPPINRSARSQASIPFLSE
                     PWYCCAEPTRDQLFSIGAQTPLVKNAVASADGFV"
     gene            6919..>7886
                     /locus_tag="VGR03_16075"
     CDS             6919..>7886
                     /locus_tag="VGR03_16075"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEV1995851.1"
                     /translation="MAINAGNSFRTRDKLNVGAQSFDIHRLEFLEKQGVADLAKLPFS
                     LRILLENLVRCEDGRSVHADDIRALGKWRPGAPEKEIAFMPARVLLQDFTGVPAIVDL
                     ATMREAVRRMGGDPKRINPLFPAELVIDHSVQVDNFGSVNAFGLNAELEFQRNVERYA
                     FLRWGQTAFQNFKVVPPDTGICHQVNLEYLARVVCAMPYGNRFEAYPDSVVGTDSHTT
                     MVNALGVFGWGVGGIEAEAAMLGQPSSMLIPEVVGFRLHGRLSEGATATDLVLTVTEM
                     LRKKGVVGKFVEFYGSGLSTLSVPDRATIANMAPEYGATMGYFPVDA"
BASE COUNT         1695 a         2274 c         2289 g         1628 t
ORIGIN      
        1 atttgccggc gacaaaggtt tcggtcttgg aagccggatt cacgttgggc tcttcgcgat
       61 agccgggcat cggcttatcg cccaattttc cgggagcata ttgtccgcga accaccgctt
      121 gcgccaccat ttccaaatcg aaggggcgga tggactgcag caccttgagt ttttcgttgc
      181 gtacggcgtt ggcatcaaag gaggcgggag gttcaacggc caccagtgag gtgagttgga
      241 gaacgtggct ctgaatcatg tcgcggaggg cgcccgttgt ttcgtaaaaa ccgccgcggc
      301 gctcgacacc gagcgtctcc gacgcggtga tttgcacatg gtcgacgtaa ttgcggttcc
      361 aaagaggctc gaaaatcccg ttgccaaagc gcagtacgag caggttttgc accgtgtcct
      421 tgccgaggta gtgatcgatg cggtagacct gcttctcgtc gaaaacgttg aggacaatct
      481 gattcaaggt cttggctgaa gccagatcgc gcccgaacgg cttctcaatg atgatgcgca
      541 cccaggaatt cggcgaggaa gggcgagaga gtccggcgcg gccaagctgc tcgacaatgt
      601 ttggataaac ttcaggtggc gtggaaagat aaaacagacg gttgccgcca atgttctttg
      661 ccgattccag ttcggcgagg cgcctggcaa gctttgcgta agcttcctga tcggcgtaat
      721 ccccgggaca gtaatgaagg ttggcagcga attcgtccca ctgtttcgga tcgatgggcc
      781 cgacttcgga gatactcttg gccgcgtcgc ccagcatggt gcgaaaggca tcttcggtca
      841 tggccgtacg cgcaaagccc acgatggtgt aacgcggtcc cagcgcattg tgaatcgcca
      901 ggtcgtacat cgcgggaatg actttgcgtt tagcaaggtc gcccgaggca ccgaagagaa
      961 ctaccgtgca gggttcaacc acgcttccgt acactgaacc ggaacgaccc atatccccag
     1021 ccatcgtctc tccctccgcc aagttgcgcc cagttcaggt acctgatgca gcgtccaaaa
     1081 ctatacctca accccatgct ttccaagctc ggaacgcaat tggtctgcgt tctgatggtg
     1141 aatggtgttc atccccaggc gccgtgggca ttctagattg agcggccggt catcaatgaa
     1201 aagacactgc tgcggcgggc gctgcgtgac ttccaacgcc acgcgaaaga tggtctcctc
     1261 gggcttccgg gatcgaacgt aacacgagct gaagaaaacc agaaagttcc ttcgaaggtc
     1321 gaaggcctca atgcggtact ggttgagctc cagcggctca ttattgatgg caccgacgaa
     1381 gtatttcccg gagctcgcaa ccttatcaag aattgcacgc gtctccgggt attctttgga
     1441 ctgcgcgaac atgaaagctg tgaactcctc gcgggtgaag ccgcgctggc gatagaacaa
     1501 tgtccggtcg agatattcat tcaaggtgat ctggccggaa tcgaaggccg ggatagagag
     1561 atcgtggcgg tcctgaaatt cttcccagtc caggtgaaaa gcatttgcgg cttcttttcg
     1621 tgatccgcgg tcccagccat ttgtcaggat aacgccgccg atgtcccaga atagcgtggt
     1681 gatctcagcc aagggcgcag ctcctccgat cttcgcgttt accttccgac catgccattc
     1741 gatgaacgag aacgcgaaga gatccgaaat ttacggcgca acgatacgcc tcttcccgag
     1801 cgcgttgttg aggatggttt ccagctgtgc caggcctggt tcggcgccac ccgtcagatg
     1861 caatcgtatt acgggtcttc gcctgcggcc aagagattcg aattcaccga gcgccagcgc
     1921 gagctgcaac tgccaaaagg aatagtccgc gcctggaatg gcaagatcct tttcgggagc
     1981 cgcggtcaaa agcaggaaga gtcctttcgc ggggccgccg aggtagacct gcccgatggc
     2041 gtgaatataa cgcgggcctg gagttaccag cacgggtagt cccagcatgg attcgagccg
     2101 ctcacgaatg cggcggaata cggctttttg aacttctcca aagttcataa aggggagcag
     2161 cgcgatgtag ccttcgcgat gccgcaagcc gaaaaaggta cgcagtgcct cggccatatt
     2221 caaggtagag atctgccggc gtgtttcggc ctcggcgtac aattcgactt ctccctctcg
     2281 aacgcgcacg gtcggcacag gagattggcg cttggtcgca atctgctcca aaatctgcac
     2341 ggttcttgtc ctgctctccc ggatgtctgg atcgtggaag ggatcgaccc cgagctgcga
     2401 acaagtaagc gccgtcgcaa tctcccattt gaatagctcg acggctagtt cctcggggcc
     2461 gttcaattcg atggtgacga tcggcacgcc ggcttcccgc aactcgtcgt atctcctgcc
     2521 gagttccggc gcttcctccc cagccatcct gagaatggcg gcgcagcagc cctgctgaag
     2581 catcgcgggc tcgtacgacg ggcttccgaa aatcgggatg attccgcgcc ccttcttgcc
     2641 ggtgcttgcc cccaccagat atccgatccg gcgcgctaca ggcttcaagc tgtccgggct
     2701 gaaaaagacc aggcggtcca ggccctcgat ttctgctgcg gccaggaacg ctccgagcga
     2761 tacggcaggg ttcgcttctt gtggcgcggc gggcccgcag gcatcgcgca tcgcttgcgt
     2821 gcgtgcgagc aagtcacttg gatccaaacg acaaagcgcc gcgagaaaaa agttaaaatg
     2881 aatcagcgat gaataacggc catggatgcc cgggggatca agaaaactgt caagaaagtc
     2941 atattcaccg gtaagttcgc ccaggtaaga attctcttcg gtcacggtga caaaatgacg
     3001 cgccggagaa tcgataccct gaattctgag tctttcaaga aagtacaaga gcaaggagtg
     3061 tgtctcgagg tgtttgcctg acttgttggc aaagataaag agagtcctat cgaggcgcag
     3121 ccactcatcg aacgcgcgga cagaatctgg atcgatgttt tcgagcagga aggttctttt
     3181 tccaagcttt gcatcaggca agcgcagaat gctcttagcc gccagattgg agtctcccat
     3241 ggtgataaaa accacatctt caaagcctgc gggttcgatc atcgcggcgc gggcggccac
     3301 gcgggccatc aatggcccca actgcccggg caaatcgagc caacgcagat tgctcttcag
     3361 ggatgctgct tggaattctt ccgccggcca gagggaagcg tccttggccc agagccgggc
     3421 gagcacatta ttggcagaaa ggttctgcaa ctcccgtcga tacgcgcttt ccagaagtcc
     3481 tggaaatatc gcttgcgtgg gtaacggcaa ggtgtctcct ggggattctg ggagtcttga
     3541 ttctactctt gttgtattga ctatttccat attttactcg cggattacaa tcggccctgt
     3601 ctccgagacg atgggagttg cgcggatcgg cgtggaaagg ccgcatgcaa ctaggaattc
     3661 tctcgaagtt gcagtaaggt gagaaggaag aacgcgtgtg aggagaagag catggcgaac
     3721 gaatcttgcg gtccaacacc ggcaccgagc gaagcggtgc aacctggcgc gcgcatggtg
     3781 cactgcgtca aatttgacaa agaattgccc ggcctggatc gcatcccgtg gcgaggagag
     3841 attggcaagc gcgtgtacga gagcgtttcg aaggacgcct ggaagctctg gctggaacat
     3901 tccaagatgg tcatgaacga gtaccggctg aatcccctcg atcccaactc gcagaaaatc
     3961 atggaagaac agatggagca gtttttcttc ggcgaaggcg ctaaattgcc cgagggatat
     4021 gttgcgccca aggccaagag ttaattccgc tggttggtca ttcggcgcgt cgcgggatgt
     4081 tcgaccggca ggactggcgt aatcgtgaac gataattttt catgggcttc gcggagcgcc
     4141 tgctcgactt tttctggcgc gcttcccctc gcaaaaagaa accccagata gcttgccccc
     4201 tcgggccagg cagcgatggc atcgtgcaat cgtgcggtga tagccagttc ggtgatcccc
     4261 ggaaccgaac gagcggcctc ttctcccgag acgccttcca gaatcccact ctctggaacg
     4321 ggaatcatca tgacaccgga ggcaattcgt tcgcatggca aattccatcc ggggagttcc
     4381 agcgcatggc gaacgagcag ctcttcgagc ccgattggtt cacttgctcc accgagggaa
     4441 aatcgcaggg ctcgggcgca gagacccccg atcggccggg gcgctacttc cagtggccac
     4501 acgccatcct cgttgattcg taactccgca tggactggtc cgtgcgacag acccaacgct
     4561 cgcacggcgt ctctcgcgca ttgctcgatg gcgcgctgtt ctgcatcggg aagccgtgac
     4621 ggcgtaacgt aaatggtctc ctcgaagtat gggccttcga gcggaaccgg cttatcaaag
     4681 atcgccaata cgctcagagc accatcggta agtagtcctt cgatcgcgac ttccctcccc
     4741 ggtatgtaac cttccacaag catctgctcg agattcgcct cgcgcgtggc aagaatctcc
     4801 ggtgatttga gtaaccgccg gacacgtgcc gctgccgcga taaactctgc acgattgttt
     4861 gcgcgcacga cgccctgact cgctgaaagc gaaagcggtt tcagcacaca ggggtacgag
     4921 atacccagca gcaccggctc gggcgtagga tcgatggcga gattccgaaa ccacggcacg
     4981 cgcagcccgg catctcgaaa gacttctttc atgcgcagct tgttccggca ggcctccact
     5041 gccgcaggat ggttgtaacg aatgcccaga ccccgggcgg cataagcggc agcggcggcg
     5101 ggccggtcac cgagagcaag aatcccgctg acatcctggc cgcgaagcgc ttccatcact
     5161 gtgtaggcag ccacctccgg cgattcgaaa tgcacggcga tggcccgatc gccccacgga
     5221 tcttccagct gatggcatcg atcggtgacg taaacaagct gcacgcccag cttgtgcgcg
     5281 gcatcttcaa aacttcgcgt ttggtaaccg agtttagcgg taaagagcag caatcgttta
     5341 gatggattcg tcatttgtga tgcgtttcta cgccgggatc aaacaaagcc atcagcgctt
     5401 gcgacggcat ttttcaccag tggcgtctgg gcgccgatgg aaaacaactg atcccgggtc
     5461 ggctcggcac agcagtacca gggttcgctg agaaacggaa tgctggcttg ggaacgcgcg
     5521 gacctattga taggaggaga aggcagtccg gctgcggcgt gcgccgcttt ccagatgcgt
     5581 tcgaatgtag ctggccgcga ttctctttgc cgctccgctg ctgcggcgat ctcctgcacc
     5641 gtttcgctga gagcgtcgag gcgcgggtcg gagtttttcc aaggatacgc cagtgactgc
     5701 ggatcgaacg gcccaacgag gcgacgcacc tcttccaatt ccagcattcg cgagccttcc
     5761 gggatgagca aacgaatacc aagctgaatg ggcgcgacat tttcgatcac cccctgttcc
     5821 tcgatcactt gcagcaaatc caggtagcca tcgactgtcg tccagggtgt gaacggcaca
     5881 aaagtcggat gaagcgtcat gccaagttca cggaacgtct cgatgacgcg caagaaatct
     5941 gcgcgcgtgt gtcccttgtc caggaatcct agcaccgcat catccacgga ttcgactgcg
     6001 ctgatcacaa agagacagcc ggtatcgcga agcgctcgca agtggttctc gtacttaagc
     6061 aagtgttcaa tcttgatggt cacgtcatat gtgatggccg gaaattcctg atgaaacgcc
     6121 tccgccaact ccatcgcgtg acgaatgccg ttaaaaaaat ccggatcgcc gaaggtgatg
     6181 tgttgtgcgc ccgcggccac ctggcggcgg acgtcttcga gcaccacgtc ccggccgacg
     6241 attcgaaaca ctcccttgta aaccggcacg atcggacaat gccggcaaag atgcttgcaa
     6301 ccgcggctcg cttccgtcga accaacgatc cgatagccat ctccgggaac gatgaggtgc
     6361 gcatacttct ccatcgcagg catccccgat cgatcaggca cttcgaactg caatcgggcc
     6421 atggagatca gggactcctc cggctggtcg atagcctgct tcccattggt ggccaagcgc
     6481 tccgccagtt gtacgagccc ttcttcaaat tcgccgccga gaattgtgga tacgccgagc
     6541 gcgcgaagat attcaacgtt catcggagcg tataggccgt aacaacagag atgcgcgctg
     6601 ggattctgtt tccgcagcgc gggaatgagt tgcgccgcca gccgcgtggc cgtatgcatg
     6661 ggcaaataga tggcaatcag cccggcatcg ccaatcgcag tttcatccag cgactgccgg
     6721 gcaaggtcga gcgccacaac gttgtggccg cgtttccgca gccatgccgc aggcgacgcc
     6781 agcccgaagg gcagcctccc caactcataa gtcgaaatga gaacgatatt cactgcccta
     6841 ccagtatagc cgcgcggcgc ttggattcaa tttcggttat tatagaaggc tgcgtatcac
     6901 agcgaaagga ctgtcaggat ggccatcaac gcgggcaatt ctttccgcac acgcgacaaa
     6961 ctcaacgtgg gcgcacagag cttcgacatt caccgcctgg aatttcttga aaagcagggc
     7021 gtcgccgacc tggccaagct ccctttttca ctgcgcatcc ttctcgaaaa cctggtgcgc
     7081 tgcgaggatg gccgctccgt ccatgccgac gacatccgcg cgctggggaa atggcggccc
     7141 ggcgctcctg aaaaagagat tgctttcatg cccgcgcgcg tgctgctgca ggatttcacc
     7201 ggggttcccg cgatcgtcga tctcgccacc atgcgtgaag ccgtgcgacg catgggcggc
     7261 gatccaaaga gaatcaatcc gctcttcccc gccgagctgg tgatcgacca ttccgtccaa
     7321 gtcgataatt ttggcagcgt caacgcgttc ggcctgaatg ccgagctgga gtttcagcgc
     7381 aacgtcgagc gctacgcgtt cctgcgctgg gggcagacgg cctttcagaa tttcaaggtc
     7441 gttccgccgg acaccggaat ctgccaccag gtgaacctgg aatacctcgc gcgggtcgtc
     7501 tgcgcgatgc cctacggcaa ccggtttgag gcttatcccg attcggtcgt gggcacggat
     7561 tcgcacacca ccatggtcaa tgcgctgggc gtctttggct ggggagtcgg cggaattgaa
     7621 gcagaggcgg ccatgctcgg ccagccctcc tcgatgctca ttccggaagt cgttggtttc
     7681 cgtctgcacg gccgcttgag cgaaggcgcg accgcgacgg atctcgtttt gaccgttaca
     7741 gaaatgttgc gcaagaaagg cgtcgtcgga aaattcgtgg agttctatgg ttcgggtctg
     7801 tccaccctca gcgttcccga ccgcgccacc attgccaaca tggctcccga gtatggcgcg
     7861 accatgggct acttccccgt tgatgc
//
LOCUS       DASYVO010000473        39130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_181529, whole genome shotgun sequence.
ACCESSION   DASYVO010000473 DASYVO010000000
VERSION     DASYVO010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 39130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39130
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181529"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..2542)
                     /locus_tag="VGR03_16080"
     CDS             complement(143..2542)
                     /locus_tag="VGR03_16080"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV1995852.1"
                     /translation="MGIAEKFMTMEYGPAPEDPKEALTWLDRYARRFGHFINGAWQAP
                     IAGKYFDTTDPSTGEKLSSVAQGSAADVNAAVEAARAALPKWQSLTPHARARYLYGLA
                     RLVQKHSRRLAVLETMDNGKPIRESRDIDIPLVARHFYHHAGWAQILEQEFPGYAACG
                     VVGQIIPWNFPLLMLAWKIAPALATGNTVVLKPAEFTPLTALAFAELCQEIALPAGVV
                     NIVTGDGTTGEALVKHPGVDKIAFTGSTEVGRAIRRATAQTHKRLSLELGGKSPFIVF
                     EDADLDSAVEGLVDGIWFNQGQVCCAGSRLLMQESIAEPLIAKVRERMSSLRVGPPLD
                     KAIDIGPIIARVQLDRIQRMVMQGVEEGATCWQPPTPMPSGGLYFPPTLLSNVHPTSI
                     VAQQEIFGPVLAAMTFRTPREAVELANNTAYGLAACVWSESINVALHVAAQLKAGVVW
                     VNCTNLFDASCGFGGYRESGYGREGGKEGLHEYLEPLWFQKAPALPPAAPIAGQESKG
                     RGSNEPEGLAAPAIDRTVKLYIGGKQARPDSGYSKEVRAADGTLLGEAPLGNRKDIRN
                     AVEAARKAEGWAKTTAHNRGQVLFYMAENLSQRGEEIARRLAAVVGKKQAAAEVSRAV
                     ERIFSYAAWTDKYDGAVHNPPFRNVAIAMNEPIGTVGIICPSEAPLLGFFSTVLPALA
                     AGNTVIAVPSEAYPLIAGDLYQVFDTSDLPAGAVNIVIGHASQLLKVLAEHDDVDAIW
                     CFTDAESGAAAKSLSTGNLKQVFTNEGRAIDWFDAQQGEGRWFLEHSTQVKNIWVPYG
                     E"
     gene            complement(2588..3655)
                     /gene="deoC"
                     /locus_tag="VGR03_16085"
     CDS             complement(2588..3655)
                     /gene="deoC"
                     /locus_tag="VGR03_16085"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV1995853.1"
                     /translation="MAARDDKRPGVEERIHLDSPAGARLTTPAATAIATLSGRPLQPN
                     RGIPLNIDRLNAVRVNTSAVERRAASHVARRTVKKDWQAAWLLRAISCMDLTTLSGDD
                     TAERVRRLCAKARRPIQQDLVEKLGIEELGIKVAAVCVYHGFVETALEALEGSSIRVA
                     AVSTGFPAGLSPFSERVAEIHRSVEAGAQEIDVVITRAHVFGGKWRELYDEVAAFKNA
                     CGPAHMKVILGAGDLLTLRNVARASVVAMMAGADFIKTSTGKEPTNATLPVGFVMTRA
                     IREFAQETGMAVGFKAAGGIRTAKQSLEWLALMKDELGLSWMQAEMFRFGASGMLADI
                     ERQLEYHATGRYSADYRHPIG"
     gene            3820..4392
                     /locus_tag="VGR03_16090"
     CDS             3820..4392
                     /locus_tag="VGR03_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995854.1"
                     /translation="MKAEFNGLKKRITAVSLLAILLFGPVAMGAKGRSSGSADVVAHI
                     SLAGNDGVVIAMRDVHYKHYLFINQPSQKAVAVVDITNAKKPRVIKTVAYPDGAQSAG
                     EIFGTNLLLVKGEKPEGRGDNTPVGVIAFGGPEGAEASQQFSGLTSFLADEDRGLVYF
                     TNADGLWVVRAKHTPRQDANDKMDLMGIYG"
     gene            4541..5347
                     /locus_tag="VGR03_16095"
     CDS             4541..5347
                     /locus_tag="VGR03_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995855.1"
                     /translation="MKNVLLLALILSCSSPLTAQTNCEEGDSPLNRNQPQGIAPHDII
                     QKFAAQEGVFKLTRDNYTYTQDVTVQTLDGHTVDGEFRQVTDISYDDKGNRVESVTFA
                     PPNTLTRVVLTQEDFDELRNRMPFVLTTEDLPQYDVLYAGTQHLDEIDTYVFDAAPKH
                     LEKDKRYFQGRIWVDDHDFQIVKTCGKIVPDTRDKKGGEHLSPSFVTYREQIDGQYWF
                     PTYARADDILHFSHDHVHIRLIMKYTRYKRFGSKSKIIFKGETPPDRKPK"
     gene            5460..8741
                     /locus_tag="VGR03_16100"
     CDS             5460..8741
                     /locus_tag="VGR03_16100"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV1995856.1"
                     /translation="MKKAFFLALILCVGAGMPAQTFRGGIQGTVTDSTGASIAGADVT
                     VKSADTGLVRTVKTDDEGNYLVTELPLGAYSVSAAKGGFGAKVLNGVSVTVSANTRAD
                     IQLSPGEVKQEVLATAEAPIIDTTGNTMGGTIQAIQMADLPVNGGDFAKLLTLVPGAG
                     ADPNGDSDSPGSFGTFSINGNRGRANNYLLDGTDMNDGYRNDPAINEAGVFGTPATLL
                     PIDSLQEIGILSNMEPEYGRNSGAVVNIVTKSGTNAIHGSAFEFFRNNALDARNAFNA
                     KPVPQDTFHNNQYGGSLGGPLIKDRTFWFVAYEGWRENGGLPASANVPSQAAVAAHGP
                     INPVIANLLARNPWSIPLPATPNDPTVPFRTVQVTDHFTNRVDSVIGKIDQHIGASDL
                     LTGRYFYGDSDQSFPLALGGGTTVPGFNTVTPTRVQVLSLSYTHMVSPRLLLEVRGGW
                     NRFAEGFFPQDSAFNPSSIGLNTSVTKSQDFGLPQISFSDGTSGLGGNNSIPRHRFDT
                     NWQYFTNVGYNTGKHNVKFGYEYRRTTVNQFYDLGYRGRLKFLSFDDFLDGNISNGGS
                     QFAGDSQRTTHQNNLGFYVQDNFRVTRKLTVNAGLRWDYIGVIYEDGNRFSIFDPTTQ
                     SLKLVGQPGGPSQLYPRDLNNFAPRLSAAYDIFGSGKTVLRAGWGLYYDAFSQDFFIG
                     HFPFNTFNPGPAYNGVGASAINSASLPASPAPTVTIASGVPIFTGFLPTSDAWTVAQN
                     IRTPYIQNFNVNIEQAMGNAMALQVGYVGSAGTKLFRFRDINQHDPATSAFPFAAFNI
                     INQFESSSYSRYHSLQVTWKLRNWRGLASQLSWTWSHSIDNASDGEDYVPNAAQPDNS
                     LNPGAEKANSNFDARHRLTWMFSYALPHPAAAKLFTNGWSLNGLLRISSGQPYNLNSF
                     SNYNNTNEFLERPDLIGNPFTGTGGPGKLLNLAAFAAPCTWDPGAGGCIPGTAHFGNL
                     GRNAFVGPNFHNFDFSLAKDTKLGERLKMQFRVDFYNIFNHPNFSNPFLPNFLVNLEN
                     NGSVTPVAGDPKCAPANGPNFVGCRAVGVGFLPTTATPDVAIGYPFLGGGGSRNIQLG
                     LRFSF"
     gene            8881..9525
                     /locus_tag="VGR03_16105"
     CDS             8881..9525
                     /locus_tag="VGR03_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995857.1"
                     /translation="MKVKTLITSLLLPAALLWGSDTKPVFNLPPATHAKTYPAHESHD
                     DERVSIAIDPFDLPEKTAVFKVNYKQNEFLPIRLIISNDADKIVMLNDLKILYITVKR
                     DKIEPAANEDIYRRLTKADARADKPRVQPPVPRKTKPAVSKEAVEEIDSLQFLTLPVA
                     AHSTHSGFLFFDISGIDTPETGAHVYVTGLKIDGKELFYFDIPLEKYLSYRPGK"
     gene            9729..10829
                     /locus_tag="VGR03_16110"
     CDS             9729..10829
                     /locus_tag="VGR03_16110"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV1995858.1"
                     /translation="MNIAIDVHSLGTQAGGNETYFRQLLGGLVADKSDNRYTLFYTHS
                     SALGETGGDRRFSFVPIPKNPIARICISLPSKLRKLKPDVFHCQYIQPPFGRTKTVVT
                     IHDLAHEHFPEYFHPLEAMRMKKLVRWTARRAAHILTVSEFSAADIARKYDVPREKIT
                     VAYQAASHSFRPREKQSCHEHLARKFGIGAPFILYVGRIQARKNLLRLVEAYASVRKQ
                     GVEAKLVLVGKKDWQFEQLVAKVEELGLQSSVIFAGFVPFEDVPLFYNAAEVFVFPSI
                     FEGFGLPVVESMASGVPTITSIGSSLEEVAGDGAMLVDPLDTAAIAAAIRQVLADREL
                     QSQLIARGLRRSAQFKRENLTQLVLQVYSSLR"
     gene            complement(10845..11546)
                     /locus_tag="VGR03_16115"
     CDS             complement(10845..11546)
                     /locus_tag="VGR03_16115"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1995859.1"
                     /translation="MARLDPLWAILSTPDKRFGNWNLDDFLRTGADEIAALMKTAGHL
                     GLPEQRRRAIDFGCGVGRLTRALRPHFAKCHGVDISPRMLEMAQRLTPECDFHEAQDL
                     GSFPNRYADLVFCNLVLQHQPHRKQAVALIADMVRVLAPGGLLVFQIPIHLPLRNRLQ
                     LRRRAYRLLRALGVRESFAYEKLRLSPIRMLCLPRADVEAITTNAGATVVRVDETRKP
                     DEPFTSGIFCCTVRP"
     gene            complement(11793..12722)
                     /locus_tag="VGR03_16120"
     CDS             complement(11793..12722)
                     /locus_tag="VGR03_16120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1995860.1"
                     /translation="MPLFPYDELKYYAFGLRAGTSNLFANGFELGLRKTIGKITQPIN
                     SYTRFPEYFFFETAIRDYLRDVPLGWPVKVLDVGSPKMLGLYLAFHFHVEVQLTDISE
                     LNLDEYRLIWRSLEPRAKGRALFVIQDARSLENREPEFDVVYSMSVVEHIDGDSGDSQ
                     AIREFLRVLKPGGLLILSVPFGNRYAEQQIVGMTGAVHKTNDSQTYFFQRIYDQPAFE
                     ERILKFAGDLANITLTAVWRRYRWVHRVLGSLGENVRGVMGFMNPLLSAAVNRSGAGM
                     QNIFSVNYGTLHTPSDLYGDLILTGRKKQSDRR"
     gene            complement(12812..13561)
                     /locus_tag="VGR03_16125"
     CDS             complement(12812..13561)
                     /locus_tag="VGR03_16125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEV1995861.1"
                     /translation="MGKLIYKSVESVVSRLKGRPYALDRSVPLSLLLGVLIRRFTWLL
                     RGFIKGLLLQRRIVLVFMASAVNLRNATLIRFGRGVTLERGVIIDGLMRGGIVLGDHV
                     MIGPYSVLTGASVANLGEGIRMGANSAVNAYSFLGSSGPISIGENVIMGQHVCFHPEN
                     HNFARTDVPIRSQGTTRLGITIEDDVWVGSNATFLDGAHVGRGSVIGAGAVVRGTIPP
                     YSIAVGVPARVIRSRKSACAGNSDAGKGVAD"
     gene            13728..14933
                     /locus_tag="VGR03_16130"
     CDS             13728..14933
                     /locus_tag="VGR03_16130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1995862.1"
                     /translation="MNIAVIMHELLVEGGGERQCVSLGRALMEQGHSVTVYTSAYDRV
                     NCFPDVCKDLNIIDVGRGWLRRLKKPFFIRGFLDMLHFKHAVNTRHEIWNPHHWPAQW
                     GAAWLKRKLGGKVVWMCNDVPNFHELARTGSARAIVHWIEYLYDRAQNRKIDLTLFLS
                     RWAENQFKLIYAGPTRVVRSGADPQRFSPGGAREKIRARYHVAPDDFLLLWLGIFMPH
                     RRLQDAIEAVKLLKDRGTRVKLLLAGSDRSYPEYLQSLRALAGRLEVNDRVIFTGKVA
                     DEEIRDYYCACDTFLFPNENQTWGLAVLEAMACGQAVLVSRGAAVQEVLTDQETALLF
                     PARDPEALSQRIQLLVADTGLRQSIAQKGMAVARSTYNWTRFAEQNVELFQEVLREEK
                     LSYEPATVR"
     gene            complement(14909..16051)
                     /locus_tag="VGR03_16135"
     CDS             complement(14909..16051)
                     /locus_tag="VGR03_16135"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV1995863.1"
                     /translation="MNNGSLRVAWDNSLVRRNPTGSGVYATQMIQELRRQPDVALEVF
                     NGWDPAKRKRGEFGSQGVLARGARAIRGLAWSYGYFPLQLRRGKFDLLHSPSFVVPFG
                     CSCPTVVTIFDVSFLMFPEHFERRWRTYMKFTMPSVLRSVSAVICISENAKQDLLKFY
                     KVSPDKVHVIYCGVDRAQFYPGAPLDQEWAESVGLHMDYVLHVGTLSQRKNIPMLLRS
                     VAALRAQGRFKDLQLVLAGPELSVLTGAPEIYDAIRDLGLGDAVVLAGFVPDECMPGL
                     YGQARLLVMPSLYEGFGLPVAESMASGVPVVASNTSSLPEIAAGAAILVPPQDESAWA
                     EGIAQILENPATAEDLRRKGLERAAQFSWQRAAAETVAVYRTVAGS"
     gene            16276..16929
                     /locus_tag="VGR03_16140"
     CDS             16276..16929
                     /locus_tag="VGR03_16140"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1995864.1"
                     /translation="MKQISLYEFGASEDDVRKIQKPWVDYFRNFGPVLDIGCGRGVFL
                     ELLAAAGIEAVGVDHSEEALAACRRKGFQVHFEEACSFQRHSAAKFGGILCSHVIEHM
                     AYDDAMIFLELCHQALRPGGRLLLITPNPEDLAVISEIFWLDPTHVRPYPKLLLKAML
                     GATGFQVIKEKQFLGSLKMIGRRNLPIYLLRRLILGRHYGKPNTLVLAKKNGNCQLK"
     gene            16948..18540
                     /gene="murJ"
                     /locus_tag="VGR03_16145"
     CDS             16948..18540
                     /gene="murJ"
                     /locus_tag="VGR03_16145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEV1995865.1"
                     /translation="MRPTLEPEAFVPPIVRRGRSVGIVGILTLLVSGFGYLREAALAA
                     RFGLSTTMDAYFAAIFIPNILYFVLIVGTLSPIFIPILLQDNADQDRAKASETFSVVT
                     TFVLLMLLILVACGMIVAPKWLPLLFVGFDHSTVQVTVRLIYIIFPAVLFLALSGILT
                     ATLNGFHRFALAAFAPALSSMAIIAVTFLARGDNAVYIVGLGTAAGFMLQGIFLVPAA
                     AAVGLHYRPRLALRHPAIKKLLRLGGPLLLYLVVANIASLLERNLASRLSAGAVSTLT
                     YAQRLFLVPASFLAAPLAIVAYPQFASEALRQGRGNLAKQVSRILRLIVFLFLPVAVW
                     TILNALPITRVLYEHGRFVLADSLLTARVLAIYGFAIIPNAVAIILLRCFFAIEDTVT
                     PLVAELIALVCFAVAAILLARQFGIEGLAAARAMSFFVVTAILMLVLSGRRALLKLDL
                     GLSGFLVRTVAAAIGMGAVNWMGLRLLQSAFDSGNTALRLGVICLLLIVSGTVYLTLA
                     RLLRLAEARQIWTTALDLIRGQ"
     gene            complement(18668..19873)
                     /locus_tag="VGR03_16150"
     CDS             complement(18668..19873)
                     /locus_tag="VGR03_16150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1995866.1"
                     /translation="MTGKRKAPIYDRKLRLAILGGRGIPSTYSGTEAFFIEIAPRLAE
                     RGHQVIVYCRKSLYKERPPEYKGVRLIYLPSIETKNLGTFTHTLACMFDVLHRDVDAM
                     LVTNVANAIHCVIPRLFRKKCALNVDGVEWKRGKWGPVGKLYFYMNARLCGKILPKGV
                     VTDAYGMRKLYLEEFGTPSACIAYGGNIEHSTNPEVVREYGLEPGNYYLILSRLVPEN
                     SADLIVRGFNKTTSRRVLAIVGDANYRSRFAADLEASAGERVRFLGRVGNTEHVRELF
                     ANAYAYLHGHTVGGTNPTLLHALGYGSCILARANVFNAEVLGDYGLLFQDEEDLAEKI
                     QMLEQKPELAESYRRRAPDRIRARYTWEHITDQYEELFYQLAAGEDPTRVHSSVRELA
                     EQPVAMSSE"
     gene            complement(19925..21340)
                     /locus_tag="VGR03_16155"
     CDS             complement(19925..21340)
                     /locus_tag="VGR03_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995867.1"
                     /translation="MSSLSPSRTVPDPTPVLFLIAIALALTPLIADESARWWLVGAAL
                     LPVGLWMFQWLTVSAEAGIALLVVAAAIPRGSVDIGGLNVRPEHIAAGLLCLAIPLLL
                     KRRTQQPAVWIYADYALAAYVGLNILSSLVVSPAPPQTLKSSMQQLLAILPYFFLRVL
                     ADDERRFRRAFRAFLVVGALAAAYGVIAYYSNFLFDTEFGVEVGQYGAIPATYGTQFE
                     ANILGAYCGAFSVAMLAMYLQEKRRIYLLGYGITLVGMAVSLSRCDLGATFIGLALLA
                     YCARKMGLLNWKVLRSIAVATLCVGIFSLPVLAPRYSERLSTLDVADPTADENTLFRF
                     VQFAAAVDDFVASPLLGNGTASFQLLFMRETAEIDIGTETPWIANTELRILHDTGVIG
                     FAAFVLFVGALVRQARRRLKRVSDPVLLALLVSAAVYAVSFQVTEGTILAFTWVHLGL
                     IACAVAVPISSGSYSVAEVGR"
     gene            complement(21489..21731)
                     /locus_tag="VGR03_16160"
     CDS             complement(21489..21731)
                     /locus_tag="VGR03_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995868.1"
                     /translation="MKVLMLHNRYLVPGGEDQSTAAEVALLTDYGHQVELLEQDNRDI
                     ESLGKARTAVRTVWSSESYRRIYEKLRAMAGYAFQG"
     gene            complement(21738..23729)
                     /gene="asnB"
                     /locus_tag="VGR03_16165"
     CDS             complement(21738..23729)
                     /gene="asnB"
                     /locus_tag="VGR03_16165"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEV1995869.1"
                     /translation="MCGIAGFWTRKTIDEHPVELLNRMGATLAHRGPDDSGVFYDNGA
                     GLGLAFRRLSIIDLSAEGHQPMASASGRYVIIFNGEVYNFEEIRAELGAHAWRGHSDT
                     EVMLEAIERRGLEAAVKRFVGMFAFALWDRHERRLHLVRDRLGIKPLYYGYVDGSFVF
                     ASELKAIRQFLGFQAGIDRDTLAAYMRCAYVPAPHSIYEGIYQLPAGHRLEMDGAEGP
                     PVMSAYWSGMEVARRGLESLVEGGDAEVIEELHQKLLAAIRLRMIADVPLGAFLSGGI
                     DSSLVVALMQSQSSRSVKTFTIGFHEGIYNEARHAKRIAEHLGTEHTELYLTPQDAQD
                     VVPLLPGMYDEPFADSSQIPTYLVSKLARRHVTVALSGDGGDELFGGYSRYAFVKRLW
                     SILRRVPGPAARGAAKLIRSVPPARIDGWLRPWPLPGRLKNSPGHKFHRLAEHLAAQD
                     PVSIYLHAISMWPDPSALVLGSHEHGTVPKAIANCSWLPTAQEMAMLTDLMNYLPDDI
                     LTKVDRASMAVSLEARVPILDHRVVEYAWRLPLRFKIRGGKTKWALRKILNRYVPPEL
                     IERPKMGFAMPVDLWIRGALREWAEDLLSQESLGRGGFFAVQPIRQKWEEHLSGTRNW
                     QYLLWPVLMFQAWLAHATSAPTARLAAGAVHLHQPSETL"
     gene            complement(23982..25331)
                     /locus_tag="VGR03_16170"
     CDS             complement(23982..25331)
                     /locus_tag="VGR03_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995870.1"
                     /translation="MSSSKIENEIQDAREDRDLSESPRKWPSWPDTLERLWNRRRRIS
                     LWVAIGFGISILLATRMCHFEATAQLMPPDSASSSLSSQVFPMLSKMSGLPNMGGGLG
                     SLAGDLLGGKNSGALFVKVLGSRTIQDSLVERFHLRQRYGKRYNEDARKILDSKTKIE
                     EDKKSGVLTIIVQDRNAQMAANLANAYVEELDRVITRVSTSSARRERMFIEQRLADEK
                     KALEEAEQRFSQFSSNTMALDVPEQIKVTVESAARLQGELIAARAELEGLRQIYSSEN
                     NRVVTLSARVGELERQLRRINSGRADPGGVQDPTFPYPSVKNLPKLGVEWADRYRESK
                     IHETVFEMLTQQYEIARIREAKEIPTVKVLDAAIVPEKRHPRPWQILLAGLVLSTLLA
                     CSGVILQDKWEKWDSRDPRRIILSRGYSGVRSLWPRRLRKPAARDDNIEQDFRQSQM"
     gene            25720..28368
                     /locus_tag="VGR03_16175"
     CDS             25720..28368
                     /locus_tag="VGR03_16175"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEV1995871.1"
                     /translation="MAALIALCSCAIHPPAAVAQQEDTSLSAEAIIQILQENPDILAE
                     AKSQIVAALRERGLAVSESDITDERLFSQIRSDDRVRLVMSDELKQRGYGLEPEAKTT
                     PQPQKPGLVPAMPETKPEAVPSKPGAPRTPGAEPKEAVKGKDNEAGRRPSQSRYPYRN
                     LPALRELYMPSVVGVENLERFGAALFRNSAVAAEKPAPDLPAGVDYVLGYGDELVIQY
                     WGSVSQRLQVSVDRQGRLTIPEAGPIMVAGRTLAEAEQLIQKALTRQYRNISVDVTLA
                     KLRMLRIYVVGDVKNPGAYEMSSLSTALNAVLIAGGPTDVGSFRTVKHFRAKKLLEEI
                     DLYDFLLRGLGAAEAHMESGDSILVPPVGPQVTVAGMVRRPAIYELLHEDTLGHVLET
                     AGGILVTGELNKIRVERIEAHERREVLSIELPEKPGAAEEAFKKFAMQDGDIVTILPI
                     TPYGNWTIYLTGHVARPGKYPYHDGVTVADMIASYQDLLPEPADRAEIVRLHAPDNRP
                     YVIPFSLRDVLEKRAPAPALNPLDTVRVYGRYEADAPKVAIYGEVIRPGEYPLSDKMT
                     ATDLVRLAGGFKRSAYADSADLASYSIVGGEQVELEHRAIHIARAMVGEPNTDAVLKP
                     GDVLTINQLGGWRNIGGAIILSGEVVHPGRYGIVAGEKISSVIKRAGGFRPEAYPNAS
                     ILERQEVREVSARNRDELIQNLQAQAVGGTTRAESIIVTRQRQQLIDRLKQIQPNGRL
                     VIHISADVAKWENTLADISVRPGDTLTIPKKPNFVMVTGQVYNPTAISFFPGKRAGWY
                     LKQTGGPTRIADKKAIFVVRSNGSVVGRNSGEWWATKTSHVVLLPGDTVYVPENIAGG
                     EKLKTFAMMAQILSGLAVAARVAISF"
     gene            complement(28498..29382)
                     /gene="rfbD"
                     /locus_tag="VGR03_16180"
     CDS             complement(28498..29382)
                     /gene="rfbD"
                     /locus_tag="VGR03_16180"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEV1995872.1"
                     /translation="MTRVVVLGANGQLGSDVSAAFAAEGHEVSALTHQDVEITSPESV
                     KLAFEKTRPEMVINAAAFSNVERCETDPVLAFQVNALGGRNVAEACCVHGAAIVHIST
                     DYVFDGAKGTPYTELDVPCPLNVYGNTKLSGEFFVRTANPRHFVVRVCGIYGRNPCRS
                     KDGLNFVERMLKLSREQEEIRVVDDEIVTPTPTVGVARQLTALSKTSGYGLYHATAEG
                     SCSWYEFARAIFDITGTRVRLERARRGEFPAKVRRPRYSVLENAALKREGLNVFRPWR
                     EGLEEYLATRETASVRRV"
     gene            complement(29529..30128)
                     /gene="rfbC"
                     /locus_tag="VGR03_16185"
     CDS             complement(29529..30128)
                     /gene="rfbC"
                     /locus_tag="VGR03_16185"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEV1995873.1"
                     /translation="MSFQIESIHLDQVFVIIPQVVGDSRGFFMETFRADRFREIGLPT
                     EFVQDNHSRSAKGVLRGLHFQWDPPMGKLMRVTSGAAFLVAVDVRKGSPTLGKWMGME
                     VSAENKKQVWAPYGFARGFCALTDECEVQYKCTGIYNPQGESSIYLGDPEIGIYWPID
                     PERGLLSERDRNAQTLAQWLASSLSDRIPYQPKPSVDKA"
     gene            complement(30343..31752)
                     /locus_tag="VGR03_16190"
     CDS             complement(30343..31752)
                     /locus_tag="VGR03_16190"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1995874.1"
                     /translation="MRNWVHFGYVLVDVLLICTNGLLAFSVHFAAIPLGVGLWHAWPK
                     LAPGFPVGQYASFLLLQTILIVLFCQSQDLYRTPRGRSMKQESWAVIRAVCFATLVLT
                     ASIFISNAKIVSREVLGTSTALSLVTLVAWRAWKRRLVTRRVAHGSSAKNTLIIGSGS
                     VAQQLARYLEENKQLGYKVLGFLDGDRGDDPRHLGRIEDLPWVARAQFVDEIFVTIPS
                     ERELVKRVVAEACRQQLNVKVVPELYDGLGWNAPIHHIGGLPVLELNRQPIPAVGLFL
                     KRLIDLTFGGVLAILGAPVMLLAALAIKLDSSGPIVHSARRVGHKGRKFQCYKLRTMV
                     ADAEAQKEELLHLNERNGATFKIANDPRITRVGRFLRRFSIDEFPQLLNVLRGEMSLV
                     GPRPHPQEDFDQYRLEDLRRLDVLPGITGLWQVSARQDASFETNVMLDLEYINNWNLL
                     FDVKILLKTIPEVLRGSGY"
     gene            complement(32165..34732)
                     /locus_tag="VGR03_16195"
     CDS             complement(32165..34732)
                     /locus_tag="VGR03_16195"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HEV1995875.1"
                     /translation="MGKGLAAGLCLFCIFLGTSEILGQEKPDAAERQADKTQSLSAGS
                     NQESAKPASKTEKSTPADTTRKNPPEPREGIWKDFFNDQKQVWTSPSRIRFADTTWLV
                     PLGGVTAGLLLTDRQLSGHLSRNPNTIQHYRTLGTAGVASLVGVGGGFYLWGKWTHED
                     HRRETGFLAGEAVINSLVVVEVMKYSTERSRPFQGDGGGKFLNGGTSFPSEHAAAAWA
                     VAGVIAREYPNPLTKLLAYGAASAVTLSRVKSRDHFPSDVLVGSVIGELVARNIYRRR
                     HDPAVGDTPWESSEEALSVKESKDARSAASPYVPLDSWVYPALDRLAGLEFVDSGFAG
                     MRPWTRRECARLLVEAQEKLGDEGTDAEETVELLKALEREFRTEMEGSESGGRGTFRV
                     ESVYSRTEYISGTPLSDGYHFGQTVINDFGRPHAEGWNTINGFSAYTTQGPWVAYVRG
                     EWQTAPSAPALPLTARQTIQVVDILPQTPPGTPRAAANQFQLLDAYVGLTLSEWELSF
                     GRQSLWWGPGQGGPMMFSDNARPINMFRVNRVTPFKLPSFLGWLGPMRMEFFLGQLSG
                     YEFVFSPTGFNGQSGVSLAQQPFIHGQKLSFKPTPNLEFGVFRTTIYGGPGYPLTSRS
                     LVRSLFSGGNQAAASPLKAGDRRSGVDFSYRLPLLRNWLTFYGDGFTDDEFSPIGYFD
                     RSAWHAGLYLSHFPGIAKLDLRVEGVYTDNPLGGALGHGFYYFNDTWRSGYQNDGNLI
                     GSWIGRDGQGAQAWGTYWLNAHSRLQTTFRHEKISQQYIPGGGTLSDFGVGGEVQMRR
                     DLSLSIRVQHERWFIPVMRPAPTDNITSSLQVLFSPEGWRRKKGRGTSTPSDAEAKP"
     gene            complement(35075..35168)
                     /locus_tag="VGR03_16200"
     tRNA            complement(35075..35168)
                     /locus_tag="VGR03_16200"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35132..35134),aa:Ser,seq:gct)
     gene            complement(35319..35876)
                     /locus_tag="VGR03_16205"
     CDS             complement(35319..35876)
                     /locus_tag="VGR03_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995876.1"
                     /translation="MYTCPDCEAEINQATEICPRCGADLAQLAQTALAAEPVKKRSLP
                     RLLLIWAAVIAVIAAGLYAFEWYVLPEYSATGAPQQAEAATVDALRVVQSQLSLFASA
                     EGHYPESLEPLGAKAKAAAQAASRGGYTVQYTPGAPEKDGTVHAYTLTARAGRYGLLS
                     FCTDQTGAIHVTRENRAATPQDPVL"
     gene            36012..36350
                     /locus_tag="VGR03_16210"
     CDS             36012..36350
                     /locus_tag="VGR03_16210"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1995877.1"
                     /translation="MKVSHVHCRVRDLPAAARWFEQVWQVAPVFHNERMVWLSFGEFG
                     VILDAAPADSVVTLSFDSEDCDADYRTVTGRGAETIEAPQDRPWGARAAYLKGPGGLT
                     VEIEQLLKRA"
     gene            36697..38112
                     /locus_tag="VGR03_16215"
     CDS             36697..38112
                     /locus_tag="VGR03_16215"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV1995878.1"
                     /translation="MKTWHKVGIGVGAVAVLGGIVLYSVNKANQGVVTVQTSKVATQD
                     LVSLVTASGEIKPTTYTNIYAEGFGKITAIVVKEGQHVKKGDTLLLQESIQQNADVQA
                     QTAALSSSDAGILSSDASYKSAQSDLQQRRSDLLRAKLDWERGQGLYKDGLIPKSDYD
                     TRKAAYDGAVAAETSSVSRVMQLQAEVDRSRAQQQQSRAMLTRTRDVLRKTNYTSPIN
                     GIVSYLPVRLGENVVPGIQNTTGSFLMTISDMSVVTSEVKVDETDIVNVKMGQEADVT
                     IDAVPGKVFKGHVTEIGSQATLRSSGLATTQSTTSTQEAKDFKVVITLEKPPDNVLPG
                     LSSTAKIKTAERKSVVAVPIQALAVRTRKDLDEAAQRAKKAKNTGESVTLAAPSPAPA
                     GTDPKKEEIQGVFIVRGKKAVFTRVETGITGVTDIEVKSGLKPGDEIVTGSYKALRTL
                     KPEADVKVDNSAPKKAEDQQS"
     gene            38176..38916
                     /locus_tag="VGR03_16220"
     CDS             38176..38916
                     /locus_tag="VGR03_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1995879.1"
                     /translation="MPAETQTADSFRQDLVSSGVVIKTEDLTKVYEMGAEKVHALRGI
                     SLEIHKGEYVAIMGPSGSGKSTLMNLIGCLDSPSSGRYWLAGRLVSELDDDELAYIRN
                     KDIGFVFQTFNLLPRATALHNVELPLIYNGTPAAERIERAKKAIEIVDLTDRMHHRPN
                     ELSGGQRQRVAIARALVNSPSIVLADEPTGNLDSQTGEEIMGVLARLHQQGNTIILVT
                     HEHDIAQHAHRSIFIKDGSIFSDQPTKK"
BASE COUNT         7964 a        12143 c        11183 g         7840 t
ORIGIN      
        1 gagcgccgcg aggatgagga tcccgctgta gaggtacggg tttcgaaagg ccgaacgcgg
       61 tttcggtttc tggtcagcct gcatgcgggc cccatgcgaa gaagtgattc caggatcagc
      121 gccccccgcc ggcggagaga attcactcgc cgtagggcac ccagatgttt ttcacctggg
      181 tcgagtgttc gaggaaccag cggccctcgc cctgctgcgc gtcgaaccag tcaatggcgc
      241 gaccctcgtt ggtgaacacc tgcttgagat tgccggtgga gagcgacttg gccgcggcac
      301 cgctctctgc gtcggtgaag caccagatgg cgtccacgtc gtcgtgctcg gcgagaacct
      361 tgaggagttg cgacgcgtgc ccgatcacga tgttcaccgc gccggcgggc agatcgctgg
      421 tgtcgaagac ctggtagagg tcgccggcga tcagcgggta ggcctccgaa ggcacggcaa
      481 tcacggtatt gcccgccgcg agggcgggga gcacggtaga gaaaaagccc agaagcggcg
      541 cctcggacgg gcagatgatt ccgaccgtgc cgatgggctc gttcatggcg atggccacgt
      601 tgcggaacgg cgggttgtgc accgctccgt cgtacttatc cgtccacgcc gcgtaagaga
      661 agatgcgctc gaccgcgcgg ctcacttccg cggccgcctg cttcttgccg acgactgcgg
      721 cgaggcgccg cgcaatctcc tcgccgcgct gcgaaaggtt ttccgccatg tagaaaagca
      781 cctgcccgcg gttgtgcgcc gttgtcttcg cccagccttc ggctttgcgc gccgcctcga
      841 cggcattgcg aatgtccttg cggttgccca gcggcgcttc gccgagcaac gtgccgtccg
      901 ccgcgcgcac ttccttgctg tagccggagt ccggccgcgc ctgctttccg ccgatgtaga
      961 gcttcaccgt gcggtcaatc gccggagccg ccagtccttc cggttcgttc gagccgcgcc
     1021 ctttcgattc ctgtccggcg ataggcgccg cggggggaag cgcgggcgcc ttctggaacc
     1081 acagcggctc gaggtactcg tgcaagccct ccttgccgcc ttcccgcccg tagccgctct
     1141 cgcgataccc gccgaacccg caggaagcgt cgaacaggtt cgtgcagttc acccagacca
     1201 cgccagcctt cagttgcgcg gccacgtgca gcgccacgtt gatgctctcg ctccagacgc
     1261 aagccgccag cccgtaggcg gtgttattcg ccagctcgac cgcctcgcgc ggcgtgcgga
     1321 aggtcatcgc cgccagcact ggcccgaaaa tctcctgctg ggcgacgata gacgtgggat
     1381 gcacgttcga gagcagcgtc ggggggaaat acaggccgcc cgacggcatg ggggtcggcg
     1441 gctgccagca ggttgcccct tcctcgacgc cctgcatcac catgcgctgg atgcggtcga
     1501 gttgcacgcg cgcgatgatc gggccgatat cgatcgcctt gtcgagcggc ggccccaccc
     1561 gcagcgagct catccgttcg cgcaccttgg cgatcagcgg ctcggcgatg ctttcctgca
     1621 tcagcagccg cgagccggcg cagcaaacct gcccctggtt gaaccagatc ccgtccacca
     1681 ggccttccac cgcgctgtcc aggtcggcgt cctcgaagac gatgaacggc gacttgccgc
     1741 ccagctcgag cgagagccgc ttgtgagtct gcgccgtggc ccggcggatg gcgcgcccca
     1801 cctcggtcga gccggtgaag gcgatcttgt ccacaccggg gtgcttgacg agtgcctcgc
     1861 ccgtcgtgcc gtcgcccgtg acgatgttca ccacgcccgc gggcaatgcg atctcctggc
     1921 agagttcggc gaaggccagc gcagtcaagg gcgtgaactc cgccggcttc agcaccaccg
     1981 tgtttcccgt cgccagcgcc ggcgcaattt tccacgccag catcagcagc ggaaaattcc
     2041 agggaatgat ctggccgacc acgccgcagg ccgcgtagcc gggaaactct tgctcgagga
     2101 tttgcgccca gcccgcatgg tggtagaaat gccgcgcgac cagcggaata tcaatgtcgc
     2161 ggctctcgcg gatgggcttg ccgttgtcca tcgtttccag caccgccagc cgccgcgaat
     2221 gcttctgtac caggcgcgcc aggccgtaga gataccgcgc ccgcgcatga ggggtgagcg
     2281 actgccattt cggcagcgcc gcgcgcgccg cctccaccgc cgcgttcacg tctgccgcgg
     2341 agccctgcgc cacgctcgag agcttttccc cggttgacgg gtccgtcgtg tcgaagtatt
     2401 tgcctgcaat cggcgcctgc caggcgccgt tgatgaagtg cccgaagcgc cgcgcgtaac
     2461 ggtcgagcca ggtgagcgct tctttcgggt cctccggcgc cggaccgtac tccatggtca
     2521 tgaatttttc agcgatgccc atgcgcttct gctcgattct ccgctcaccc aagcctcatg
     2581 cccggctcta gccgatcgga tgccggtaat ccgccgagta gcgtcccgtg gcgtgatact
     2641 ccagttgcct ctcgatgtcc gccagcatgc cgctcgcgcc gaagcggaac atctccgcct
     2701 gcatccacga cagccctagt tcgtccttca tcagcgccag ccattccagg gattgcttcg
     2761 ccgtgcggat cccgcccgcg gccttgaagc ccacggccat gccagtttcc tgggcgaact
     2821 cacggatggc ccgcgtcatc acgaatccga ccgggagcgt cgcgttggtc ggctcttttc
     2881 ccgtggacgt cttgatgaag tcggcgcccg ccatcatggc cacgacgctg gcccgcgcga
     2941 cgtttcgcag cgtcagcagg tcgcccgcgc cgagaatcac cttcatgtgc gccggaccgc
     3001 aagcattctt gaatgccgcg acctcgtcgt aaagctcccg ccacttgccg ccgaagacgt
     3061 gcgcccgcgt gatgaccacg tcaatctcct gcgcgcccgc ctccaccgaa cggtggattt
     3121 ccgcgacgcg ctcggagaac ggagaaagcc cggcggggaa tcccgtcgag acggcggcga
     3181 cgcggatgct gctgccctcg agcgcttcga gcgccgtttc gacaaagccg tgatagacgc
     3241 agaccgccgc gactttgatc cccagttctt cgatgccgag tttttcaacg aggtcctgct
     3301 ggatgggccg gcgcgccttg gcgcaaagcc gccgcacgcg ctctgccgtg tcgtcgccgg
     3361 agagcgtggt caagtccatg caggagatgg cgcggagcag ccacgccgcc tgccaatctt
     3421 tcttcaccgt gcggcgcgcg acgtggctcg cggcgcggcg ctcgaccgcg ctggtattga
     3481 cgcggaccgc gttcagccgg tcgatattga ggggaatccc ccggttcggc tgcagcggcc
     3541 gcccggaaag cgtggcgatg gcggtagctg caggagtcgt aaggcgggcg ccggccgggc
     3601 tgtccagatg aatgcgctct tcgacgccgg gacgcttgtc gtctctcgcc gccattcgtt
     3661 cactcctcta tgtggaattg tatagcagat tccgcaacgc gcgcagggtt ccctccggcg
     3721 ccgcgaggga ggcgcaagga gagggaataa aacctgctgc agcttagtca tgaatggtgc
     3781 ggggccggca ccgtaattca gattaaagag agggatatca tgaaagcgga gttcaacggg
     3841 ctaaagaaga gaatcactgc ggtctcattg cttgcgatcc tgcttttcgg ccccgtggca
     3901 atgggcgcga aaggacggag cagcggatcg gcggatgtcg tcgcgcacat ttccctggcg
     3961 gggaacgatg gcgtggtcat cgcgatgcgt gatgtgcatt acaaacacta cctgttcatc
     4021 aaccaaccgt cgcagaaagc ggtggcagtg gtggacatca ccaatgcgaa gaagcccaga
     4081 gtcatcaaga ccgtggcgta tcccgacggc gcacagagcg ccggcgagat cttcggaacg
     4141 aatcttctgc tggtgaaggg cgagaagccg gaaggccgcg gcgacaacac tcccgtggga
     4201 gtcatcgcct tcggcggtcc ggaaggagcc gaggcttcgc aacagttcag cgggttgacg
     4261 agcttcctcg cggatgagga tcgcgggctg gtctatttca ctaacgcgga cgggctgtgg
     4321 gttgtgcgag caaagcacac tccgcggcag gacgcgaacg acaagatgga cttgatgggc
     4381 atctacggct gaattgcagc gcaggctgca gcggggcagg cctccgggcc tgccccgttt
     4441 tttttgtact ggttcattcc gtagcccgca tggctggctt tctccggagg gtataatccg
     4501 acgagggacc cagggagcag aatcctgtga gagagacgcc atgaagaacg tgcttctact
     4561 ggctttgatt ctttcttgca gcagtccact gaccgcgcaa accaattgcg aggaaggcga
     4621 cagtccgctg aaccggaacc agccccaggg catagcgcct cacgacatca tccagaagtt
     4681 tgccgcccaa gagggcgttt tcaaactgac ccgcgacaac tacacctata cccaggacgt
     4741 gaccgtgcag accctggacg gccacacagt ggacggggag ttccgccagg ttaccgacat
     4801 cagctacgac gacaagggca accgcgttga gagcgtgacc ttcgctcctc cgaacacgct
     4861 gactcgcgtg gtcctgaccc aggaagactt cgacgaactc cgcaatcgca tgccttttgt
     4921 cctgaccacg gaggacctgc cgcaatatga cgtcctgtat gccggcacgc agcatttaga
     4981 tgagattgac acttacgtgt tcgatgccgc tcccaaacat cttgaaaaag acaaacgcta
     5041 tttccaggga cgcatctggg tggacgatca cgatttccaa atcgtcaaga cctgcggcaa
     5101 gatcgtgccg gatactcgcg acaagaaagg cggggaacac ctctcaccca gttttgtgac
     5161 ctatcgcgag cagatcgacg gccagtactg gttcccgacc tatgcgcgcg ctgacgacat
     5221 tctgcatttc agccacgacc acgttcatat tcggctcatt atgaagtaca cgcgttacaa
     5281 gcgctttggg tccaagtcaa agatcatctt caagggtgag acgccgcctg accgaaagcc
     5341 gaagtaagag tcgcgggccg gccggagccc aagctccgtc acactgcttt cgcttgattt
     5401 tcaggaagcg tataatcccg caggggaccg gggaccaaat tccttcgaaa gagatgacca
     5461 tgaagaaagc atttttcctg gctttgatac tttgcgttgg cgccggcatg ccggcgcaga
     5521 catttcgcgg cgggatccag ggaactgtca ccgactcgac cggggcgtca attgccgggg
     5581 ccgacgttac tgttaagagc gcagacacag ggctggtccg cacggtcaag acggatgacg
     5641 agggcaacta tcttgtcaca gagctgcctt tgggagccta tagcgtcagc gcagcaaaag
     5701 gcggtttcgg cgccaaagtg ttaaacggtg tcagcgtgac ggtttccgcc aacacgcggg
     5761 ccgacatcca actcagcccc ggcgaggtca aacaggaagt gctggccacg gcggaggcgc
     5821 ccatcatcga taccaccggc aacaccatgg gcgggacgat tcaggccatc caaatggccg
     5881 atctgccggt taacggaggc gatttcgcca agctgctgac cctggttccg ggcgcgggcg
     5941 ctgatcccaa tggcgacagt gattcgcccg gatcgttcgg cacctttagc atcaacggca
     6001 accggggacg cgccaacaac tacctgctcg acggcacgga catgaatgac ggctaccgca
     6061 acgatcctgc catcaatgag gccggcgtgt tcggcacgcc tgcaaccctg ttgcctatcg
     6121 attcgctgca ggagatcggc atcctctcca acatggaacc tgaatatgga cgaaactccg
     6181 gcgcggtggt gaacattgtc accaagtccg gaacgaatgc gatccatggt tccgcgtttg
     6241 agttctttcg caacaacgct ctcgacgcgc gcaacgcgtt caacgccaag ccggtcccgc
     6301 aagacacctt ccacaataat caatatggcg gctctttagg cggcccgctg atcaaagacc
     6361 gcaccttctg gtttgtggcg tatgaaggat ggcgggaaaa cggcggcctg ccggcgagcg
     6421 ccaacgttcc gagtcaggca gccgtggcgg cgcacggtcc gatcaacccg gtcatcgcga
     6481 acctgcttgc gcgcaatccc tggagtatcc ctctgccagc cacgcccaac gatcctactg
     6541 tgcccttccg caccgtacag gtcaccgatc atttcaccaa tcgtgtcgat agcgtgattg
     6601 gcaagattga ccagcacatc ggcgcgagcg accttcttac cggccgttat ttctatggtg
     6661 acagcgatca gagctttcct ctggcgctgg gtggcgggac cacggtccct ggctttaaca
     6721 ccgtcacgcc cactcgcgtg caggtgctct ccctttctta tacgcatatg gtttcgccga
     6781 gattattgct ggaagtccgc ggcggatgga accgctttgc cgaaggattc ttccctcagg
     6841 acagcgcttt caatccctcg agcatcggcc tgaacaccag cgtgaccaag tcgcaagact
     6901 tcgggttgcc gcagatcagc ttcagtgacg gcaccagcgg actgggcggc aacaattcga
     6961 ttccgcggca ccgctttgac accaactggc aatacttcac caacgtgggt tacaacactg
     7021 gaaaacacaa tgtgaagttc ggctatgagt atcgccgcac cacggtgaac cagttctacg
     7081 atctgggata tcgcgggcgg ctgaagttcc tgtcgttcga tgattttctc gacggaaaca
     7141 tcagcaacgg cggcagccag tttgccggcg attcacaacg caccacccac cagaacaact
     7201 tgggcttcta cgtccaggac aacttccgcg ttacgcgaaa actcaccgtc aacgcgggcc
     7261 tgcgttggga ttacattggc gtgatctatg aggatgggaa tcgcttcagc atcttcgacc
     7321 ccacgacgca gtcgctgaag ctggtgggcc agccgggcgg tcccagccag ctctatccca
     7381 gggacctgaa caacttcgcc ccgcgcctga gcgctgctta tgacatattc ggcagcggga
     7441 aaacggtcct tcgcgccgga tggggcctct actatgacgc cttctcccag gactttttca
     7501 tcggacattt ccccttcaat accttcaatc ccgggccggc gtataacggt gtgggcgcat
     7561 cggcgatcaa ctccgcaagc ttgcccgcat cccctgcccc gacggtcacc atcgcttcgg
     7621 gagtgccgat cttcactggc tttcttccca cgagcgatgc atggacggtg gcccagaata
     7681 tccgcacccc ttacatccag aatttcaacg tcaacattga gcaggccatg ggcaatgcga
     7741 tggccctgca ggtgggttat gtgggctcag ccgggaccaa gctgttccgc ttccgcgaca
     7801 tcaaccagca tgatcccgcc accagcgcat ttccgtttgc tgcgttcaat atcatcaacc
     7861 agtttgaatc cagctcatat tcccgttacc acagcctgca agtcacgtgg aagttgcgca
     7921 actggcgcgg acttgcctcg cagctctcct ggacgtggtc gcactccatt gacaacgcca
     7981 gcgacggcga agactacgtg cccaacgccg cgcagcctga caacagcctc aatcccggag
     8041 cggaaaaggc aaattcgaat ttcgacgcgc gccaccgcct gacgtggatg tttagttatg
     8101 cgctgcccca tcccgccgcc gcaaaattgt ttaccaatgg ctggtcattg aatgggctgc
     8161 tgcgcatctc cagcggccag ccttataacc tgaactcgtt ctctaattac aacaacacca
     8221 acgagttctt ggagcggcct gatttgattg gcaatccttt taccggaacg ggtggtccgg
     8281 gcaaactgct caacctggcc gctttcgccg cgccgtgcac ctgggacccg ggggcggggg
     8341 gatgcattcc cggtaccgca catttcggca acctggggcg caatgctttt gtcggaccga
     8401 actttcacaa ctttgatttc tcgctggcca aggacaccaa gctgggagaa cggctgaaga
     8461 tgcagttccg ggtcgacttc tacaatatct tcaaccatcc caacttctcc aacccgtttc
     8521 ttcccaactt cctggtcaac ctggagaaca atggcagcgt gacgccggtc gcgggcgacc
     8581 ccaagtgcgc gccggcgaat gggccgaact ttgtcggctg ccgcgcggta ggggtggggt
     8641 tcctgcctac cactgcgacg ccggatgtag cgattggcta cccgttcctg ggcggcggcg
     8701 gctcgcgcaa tatccagctc ggtctgcgct tctccttcta gactaaacga gactagatca
     8761 gaatctttgc agcagaagcc ccccgatggg gcaagggcgc tccatccggg gtcgtcgcgt
     8821 tcggcggccc tgcgggggcc aaaggtccgc agcagttcac cgcatggacg agctaaattc
     8881 gtgaaggtga agactctcat cacttctctc ttactgccgg cggcgctcct ctggggcagt
     8941 gacaccaagc cggttttcaa tctgcctccg gccactcacg ccaaaaccta cccggcacac
     9001 gaatcccatg acgatgagag agtctccatc gccattgatc cttttgattt gcctgaaaag
     9061 acagcggttt tcaaggtgaa ctacaaacag aacgagtttt tgcccatccg gctgatcatc
     9121 tccaatgatg cagataaaat tgtcatgctg aatgatttga agatcctgta catcacggtg
     9181 aagcgcgaca agatcgagcc cgccgccaac gaggacatct accgccgcct gacaaaggcc
     9241 gacgcccgtg cggacaagcc gcgcgttcag ccacccgtcc cccgaaaaac caagcctgcc
     9301 gtcagcaagg aagccgtgga agaaattgat tcactgcaat ttctcaccct tcctgtcgcc
     9361 gcacattcaa cccacagcgg tttcctcttc ttcgacatct ctggaattga cacgcccgag
     9421 accggcgctc atgtctatgt gacgggcctc aaaatagacg gcaaggagct tttctacttc
     9481 gatattccgc tggaaaagta cctgagctac cggccgggca aatgaagccg atttgcggaa
     9541 cgcgagcccg ccattgagat tcagaacagt gagatcccca gtcccgtgac tttgcgaatt
     9601 ccatgccgtt atactgacct tgagaatatc tgagagagaa agaagaacag agtacgcgtt
     9661 ggtgaagcgg tgccggacat ttgccagcct gtcaatcaga gtgctctctc cagtgctcct
     9721 catactcgat gaacattgca attgacgtgc acagtttggg aactcaggct ggaggcaacg
     9781 aaacctactt tcggcaactc ctcggggggc tggttgccga caagagcgac aatcggtata
     9841 ccctcttcta cacgcattct tccgcactgg gcgaaaccgg cggcgaccgg cggttcagct
     9901 tcgtgcccat tcccaagaac cctattgccc gcatctgcat ctcgcttcca tccaagctgc
     9961 gcaagctcaa accggatgtt ttccactgcc aatacattca accacctttt ggaaggacaa
    10021 agaccgttgt caccattcac gatctggccc atgagcattt tcccgaatac ttccatcccc
    10081 tggaggcgat gcggatgaaa aagctggtcc gctggacagc ccggcgcgcg gcccacatcc
    10141 tgactgtgtc tgagttctcc gctgcagaca tcgcacgtaa atacgatgtc ccacgcgaaa
    10201 aaatcaccgt ggcttaccag gcggcatctc acagctttag accgagagag aaacaatctt
    10261 gccacgagca tctcgctcga aaattcggaa tcggcgcccc tttcatcttg tatgtgggcc
    10321 gaattcaggc gcggaagaac ctgctccggc tggtggaagc ttatgcgagc gtgcgcaaac
    10381 agggcgtaga ggccaagctg gtgctggtgg gaaagaaaga ctggcagttt gaacaactgg
    10441 tcgcgaaggt tgaggagctc ggattgcagt cttccgtgat tttcgccgga ttcgtgcctt
    10501 ttgaagacgt gcccctgttc tacaatgcag ctgaggtttt cgtcttccct tctatcttcg
    10561 aaggattcgg tctgccagtg gtcgagagca tggcgagcgg cgtcccgacc atcacgagta
    10621 ttgggtcctc cctggaggaa gtagccgggg acggggcgat gctggtcgat ccgctggaca
    10681 ccgcagcgat cgcggccgcg attcgccaag ttcttgcaga ccgggagctt caatcacaat
    10741 tgatcgcccg cgggctacga cgaagcgcac aattcaaacg tgaaaacctg acgcagcttg
    10801 tgcttcaggt atattcctcg ctccggtaga ttccgcgtgt tctttcaggg tcttaccgtg
    10861 cagcaaaata tgccgctggt gaagggctcg tcaggcttcc tagtttcatc cacgcgaacg
    10921 accgtggcgc ctgcgtttgt agtgatggct tccacgtcgg cccgtggcag gcaaagcatg
    10981 cgaatgggag acagcctgag cttttcgtaa gcgaaggact cccgtactcc caaagcgcgc
    11041 agaagacggt atgcccggcg acgtagctga agccggttgc gcagcggaag atggataggt
    11101 atctgaaaga cgagcaagcc tcccggcgca aggacccgca ccatgtcggc tatcagcgcc
    11161 accgcctgct tcctgtgggg ctgatgctgc aggaccaaat tgcaaaacac aagatcggcg
    11221 taccgattcg ggaatgaccc caggtcctga gcctcatgga aatcacactc cggggtgagg
    11281 cgctgggcca tctccagcat acggggagaa atgtccaccc cgtgacattt tgcgaagtgg
    11341 gggcgcagcg ctcgcgttag cctccccacg ccacaaccga aatcgattgc acggcgtcgt
    11401 tgctcaggca gtcccaaatg cccggcagtc ttcatcagcg cggcaatctc gtctgctccg
    11461 gtgcgcagaa aatcatctag attccagttc ccgaagcgct tgtctggagt ggaaagaatt
    11521 gcccagaggg ggtcaagtct ggccatttcc tcccattcgc gcgcatggag tgaactggct
    11581 ggcatcggtg acagtttaac tgaggtcacg cttagcttag ctcatttgaa taatgcggcc
    11641 atctgccgga tcactcccgg gctgcggcga aacgcacaat tcaaacggga gaatcttgcc
    11701 caacgcgtaa ttgaggtgta caggtcgctt taagggctcc ctgcgaaaga gaaatgcaag
    11761 acagccgtat cccgggcgat cactcccttc tgtcaacggc gatcagattg cttcttgcgg
    11821 ccggtcagaa tgaggtcccc gtacaaatcg ctgggcgtgt gaagggtccc ataattcacg
    11881 ctgaatatgt tttgcatgcc tgcgccggag cggtttacgg cggctgaaag caaagggttc
    11941 ataaatccca ttaccccgcg cacgttttca cccaaagagc ccaggacgcg gtgaacccac
    12001 ctgtacctgc gccaaacggc agtcagagtg atgttcgcca gatcgccagc gaatttaagt
    12061 attcgttctt caaaagccgg ctggtcatag atgcgctgga aaaaataggt ctgagaatcg
    12121 ttggtcttgt gcacggcccc cgtcattccc acgatctgtt gttcagcgta tctgtttccg
    12181 aaaggaacgc tcaggattag caagccgccg ggtttcaata ctcgcaggaa ttcccggatg
    12241 gcttgggaat ctcctgaatc gccgtcgatg tgttcgacta cggacattga gtatacaacg
    12301 tcgaattcag gctcacgatt ttcaagcgaa cgcgcatcct ggatgacaaa cagggccctg
    12361 ccctttgccc tgggttccag gcttcgccag atcaggcggt actcatccag attcaattca
    12421 ctgatgtccg tgagctgcac ttcgacgtga aaatgaaatg ccaggtagag acccagcatc
    12481 ttgggactgc cgacatccag taccttgacg ggccagccaa ggggaacgtc gcggaggtaa
    12541 tcacgaattg cggtttcaaa aaaaaagtat tcggggaagc gggtgtagga gttgatgggt
    12601 tgggttattt tgcctatcgt tttccgcagt cccagttcaa agccgttggc gaacaggttg
    12661 gacgtccccg cccggagtcc gaaggcgtaa tacttgagct cgtcataggg gaataggggc
    12721 attcttgatc tggctccttc gccggcgcgg gaaggctttt ctgcggctct tcggctgagt
    12781 ctcacaagag aattgcagag cgctcgcggc tctagtctgc gacgcccttc ccggcatctg
    12841 aattacctgc gcatgctgat tttctggagc gaatcactcg agcggggacg cccaccgcaa
    12901 tcgaataggg cggaatcgtc ccgcgcacta cggcgccggc gccgatgaca gatcctcggc
    12961 ccacgtgtgc tccatccagg aaagtcgcgt ttgagcccac ccacacgtca tcttcgattg
    13021 tgatgcctaa cctggttgtc ccctgagacc ggattggcac gtctgtccga gcaaagttgt
    13081 gattctccgg gtgaaagcaa acatgttggc ccatgatcac attctcgccg atagaaatgg
    13141 ggccggaact cccgaggaac gagtaagcat tcaccgcgga gtttgcgccc atcctgattc
    13201 cttcgcccag attggcaacg gacgcccctg tcaaaaccga gtaaggacca atcatgacgt
    13261 gatcgcccag gacaatgccg ccacgcataa ggccgtcaat gatcacaccg cgctcgagtg
    13321 tgactccccg gccgaaacgg atgagagttg cgttccttaa gttgaccgca ctcgccatga
    13381 atacgaggac gattcgccgc tgcagcaaca gtcctttgat aaacccgcga agcagccaag
    13441 tgaaacggcg aatcaacacg cccaacagaa gcgaaagagg gacgctcctg tccagggcgt
    13501 agggccttcc cttcagtcgg ctgacaacag attccactga tttgtagatc agcttaccca
    13561 ttcaactgcc tctcgccgat atgagcttgg cttgttgagc ggccagtcta gcaccatccc
    13621 ggatcgtctc cctcctgagc tcgaagaagg ggcgccctcc aagctccagg gtatgcccgc
    13681 gagtttgtaa gatactatag agaggaaaat ttttcgttgc acgcaaaatg aacatcgctg
    13741 taatcatgca cgaattgctg gttgaaggag gcggcgagag gcagtgtgtc tcacttgggc
    13801 gggccctgat ggagcagggc cactccgtca cggtctatac gtccgcctac gatcgggtca
    13861 actgttttcc ggatgtttgc aaagatctca acatcataga cgtgggccgg ggatggcttc
    13921 gccggctcaa gaaacccttc ttcatccgcg gcttcctcga catgctgcac ttcaaacacg
    13981 ccgtcaacac acggcacgag atttggaacc cccatcactg gcctgcgcag tggggcgccg
    14041 cgtggctgaa acgcaagctg ggaggcaaag tcgtctggat gtgcaacgat gtccccaact
    14101 ttcacgaact ggctcgtacg ggatcggccc gcgccatcgt gcactggatc gaatacctgt
    14161 acgatcgcgc acagaaccgc aagattgacc tgacgctgtt tctgtcccgc tgggcagaga
    14221 accaattcaa attgatctac gcgggcccta cgcgcgtggt gcgcagcggc gccgatcccc
    14281 agcggtttag cccaggcgga gcgcgcgaaa agatccgcgc gcgttaccat gtcgcgcctg
    14341 acgatttcct gctgttgtgg ctgggaattt tcatgcccca tcggcggctg caggatgcaa
    14401 ttgaagcagt gaaactcctg aaagacagag gtacccgggt caagcttctg ctggccggct
    14461 cggaccgcag ctacccggaa tatctccagt ccctcagagc gctcgcaggc cgcctggagg
    14521 taaacgaccg cgttatcttc accggcaagg tcgccgacga agagattcgc gattattatt
    14581 gcgcctgcga cacttttctc tttcccaacg aaaaccaaac ctggggactt gctgtcctgg
    14641 aggccatggc ctgcggccaa gcggtgctgg tctcacgagg cgccgccgtg caagaagtgc
    14701 tgaccgacca ggaaaccgct ctgctgttcc cggcgcgcga tcctgaggcg ctctcgcaga
    14761 gaatccagtt gctggtcgcc gacactggcc tccggcagtc catcgcgcaa aagggcatgg
    14821 cggtggcccg cagtacctac aactggaccc gcttcgccga gcagaacgtg gaactcttcc
    14881 aggaagtgct gcgggaagaa aaactgtcct acgaaccagc gactgtgcga taaacggcca
    14941 ccgtttccgc cgccgcccgt tgccaactga actgagcggc gcgctccagg cctttgcggc
    15001 gcagatcttc ggccgtggcc gggttttcca ggatctgggc aatcccctcc gcccacgcgg
    15061 actcgtcttg cggcgggacc aggatggccg ctcccgcagc aatctccggc aaactggacg
    15121 tattgctggc caccaccgga actccgctgg ccatgctctc cgccaccggc agcccgaacc
    15181 cttcgtaaag agaaggcatg acgagaagtc tggcctgccc gtacaagccg ggcatgcatt
    15241 catccggaac aaagcccgcg agcactaccg catcgccaag ccccaggtcg cggatcgcat
    15301 cgtaaatctc gggggccccg gtgagcaccg aaagctccgg accagccagc accaactgca
    15361 ggtccttgaa cctcccctgg gcccgcaggg ccgccaccga ccgcagcaac atagggatgt
    15421 tttttcgttg tgaaagagtg ccgacgtgaa gcacgtaatc catatgcaat cccacgctct
    15481 ccgcccactc ctggtcgagg ggcgcgcccg gataaaattg cgcacggtca acaccgcaat
    15541 agataacatg caccttatcc ggcgacacct tgtagaactt aagcaaatcc tgtttagcgt
    15601 tctctgagat acagataaca gcggacaccg aacgaaggac cgacggcatc gtaaacttca
    15661 tataagtccg ccagcgccgc tcaaaatgct ccggaaacat gaggaagctc acgtcaaaga
    15721 tggtaaccac agtcgggcag gaacatccaa agggcaccac gaaggaagga gagtgcagaa
    15781 gatcaaactt gcccctacgc aactgcagcg gaaaataccc gtagctccag gccagccccc
    15841 ggatcgcccg ggccccccgc gccagcaccc cctggctgcc gaattccccc cgcttccgct
    15901 tcgccgggtc ccagccgttg aatacttcca gggccacatc cggttgccgg cgcagctcct
    15961 ggatcatctg cgtcgcatag acaccgcttc ccgtcgggtt tcgccggacc agcgagttgt
    16021 cccatgcgac gcgcaaagaa ccgttgttca tcggccgtta tgcttgctcc tcttcctgaa
    16081 cgagctcata tctagttggt agcagtgaat ttacaccgtg cggatgcggt ttctataata
    16141 cagctcgccg ggggatacaa gcgctggaaa ctgcaccgcg gcaattatca cgcggatttc
    16201 gcacatggct gatgggattt ccgccaaaca tccccgcccc gggaacattt tgttcgtact
    16261 gacggtctag accagatgaa gcaaatcagt ctctacgagt tcggcgccag cgaagatgac
    16321 gtgcgcaaga tccagaagcc gtgggtggac tactttcgca acttcggccc ggtgctggac
    16381 atcggttgtg gccgcggcgt cttcctggaa ttgcttgcgg ctgcagggat cgaagccgtg
    16441 ggcgtcgacc attccgaaga agcgctcgcc gcctgccggc ggaagggctt tcaagtgcac
    16501 ttcgaggaag cctgtagttt ccagcgccac agtgcggcga agttcggcgg aattctctgc
    16561 agccatgtaa ttgagcacat ggcctatgac gacgccatga tttttctgga actctgccat
    16621 caggcgctgc gccccggcgg acggcttctg ctaatcaccc ccaatcccga agatctggcc
    16681 gtgatatcag agattttttg gctcgaccca acccacgtgc ggccctatcc aaaactgttg
    16741 ctcaaggcga tgctcggcgc tacaggcttc caggtgataa aagaaaagca gttcctggga
    16801 agcctgaaga tgatcgggcg tcgcaatctt cccatatacc tcttgcggcg actgattctc
    16861 ggacggcatt atgggaaacc caacacgctg gtgctggcga agaagaatgg caactgccag
    16921 ctgaagtaaa gtagagaaaa tcgattggtg cgacctactt tagaacctga agcgttcgtc
    16981 ccccccatcg ttcgccgggg acgcagtgta ggcatcgtcg gaattctcac gctcctcgtt
    17041 tccggcttcg gttatctccg cgaagctgct ctggccgccc ggtttggctt gtccaccacc
    17101 atggacgcgt atttcgccgc aatttttatt cccaacattc tctacttcgt tctgattgtg
    17161 ggaactttgt cgccgatttt catcccgatt ttattgcagg acaacgccga tcaggaccgg
    17221 gccaaagcgt cagaaacttt cagcgtcgtc accacttttg tgcttctgat gctgttgatt
    17281 ctggtggctt gcggaatgat tgtggccccc aagtggctac cgctgctgtt cgtcggcttc
    17341 gaccacagca ccgttcaggt tactgtccgt ctgatttaca tcatttttcc ggcagtgttg
    17401 ttcctggctt tgtccggcat tctcacggcc acgctgaatg gttttcatcg ctttgcgctg
    17461 gcggccttcg cccctgcact ctccagcatg gcgatcatcg ccgtcacctt cctggcccgg
    17521 ggtgacaacg cggtttacat cgtggggttg gggactgccg ccggctttat gctgcagggg
    17581 atattcctcg tcccagccgc cgcagcggtc ggacttcatt atcggccccg ccttgcgctt
    17641 cgtcatccgg ccatcaagaa gctgttgcgc ctaggcgggc cattgcttct ttatctggtg
    17701 gtcgccaaca ttgcttccct gctggaacgc aatttggcct ctcggctttc cgccggggct
    17761 gtatcgacct tgacctacgc tcagcgcctc ttcctggtgc ccgccagctt tctcgcggcg
    17821 ccactggcca tcgtcgctta tcctcagttt gccagtgaag ctttgcgcca gggccgtggc
    17881 aacctggcca agcaggtctc ccggatactc cggctcatcg tgtttctgtt cttaccggtc
    17941 gcggtgtgga ccattctcaa cgctctgccg atcacccgcg tcctctacga gcatgggcgg
    18001 tttgttctgg cagactccct gttaacggca cgggtgctgg ctatctatgg cttcgcaatc
    18061 attccgaacg ccgtggcgat catcctgcta cgctgctttt ttgccatcga agacacagtc
    18121 actccgctgg tggccgaact gatcgccctg gtgtgctttg ccgtggccgc aatcctgctt
    18181 gcccgccagt tcggcattga aggtctggct gcggcccgcg ccatgtcgtt ctttgtcgtg
    18241 acggcaattc tcatgctcgt cctttcgggc cggagggcct tgctgaaact tgacctcggc
    18301 ctatcagggt tcttggtccg cactgtggcc gccgcaatcg ggatgggggc agtgaactgg
    18361 atgggactgc gcctcctgca atcggcgttt gattccggca acacggcgct ccggctgggg
    18421 gtgatctgtc ttctcttgat cgtcagcggt acggtctacc tgacacttgc tcgcctgttg
    18481 aggctggcgg aggcccgcca gatatggacc acggccctgg acctgatacg cggccagtaa
    18541 tccaagaaat ggaccatctt tagcgggacc ggagcgtcgt cagggcttga ttttccacca
    18601 ccatttcttc ccagctgcgc ttatggagcg gaacagttgg tattgtttcg cacgataaac
    18661 aaacttctta ttcagaggac atcgcgaccg gctgctccgc aagctcgcgg acactggaat
    18721 ggacccgggt gggatcttca cccgccgcca gttggtaaaa caattcctca tactggtcgg
    18781 tgatgtgctc ccatgtatag cgcgccctga tccggtccgg cgcgcgccgg cgataactct
    18841 ctgcgagttc cggcttctgt tcgagcattt gtatcttttc agccaggtcc tcctcgtcct
    18901 gaaacagcag gccataatct ccaagtactt cagcattgaa cacgttggca cgggccagga
    18961 tgcaactgcc atagccaagg gcatgcagca aggttggatt tgttccgccg acagtgtgac
    19021 cgtggagata ggcgtaggcg ttcgcaaaca attctcttac gtgctccgtg ttgcccacgc
    19081 gccccagaaa gcgcacccgc tcacctgcgc tggcctcgag gtcggcagcg aaccggctcc
    19141 tgtaattggc gtctcctacg attgccagca cacgcctgct cgtcgtctta ttgaagcctc
    19201 tcacgatcag gtcagcgctg ttctccggta ccagacggct gaggatcagg tagtaattgc
    19261 cgggttccag accgtactcc cgcacaactt ccggattggt cgaatgctca atgtttccgc
    19321 cataagcgat acacgcagag ggggtgccaa attcttcgag atacagtttc ctcatgccgt
    19381 aggcgtccgt aacaacaccc ttgggcagga tcttgccgca aagccgcgcg ttcatgtaaa
    19441 agtacaactt tcccaccggc ccccatttgc ctcttttcca ttcgactcca tccacattga
    19501 gggcgcattt ctttcggaag agacgcggga tgacgcaatg aatggcattc gccacgttgg
    19561 tgaccagcat ggcatctacg tcgcggtgca ggacatcgaa catgcacgct aaggtgtgag
    19621 taaacgtccc caggttcttg gtctcaatgc tgggcaggta gatcagccgc acgcccttat
    19681 actcaggcgg ccgctctttg tacagtgact tccggcaata gacgatgacc tgatgcccgc
    19741 gctcggccag acgcggggcg atttcgataa aaaaggcttc agtgccgctg taggtcgagg
    19801 gaatgccacg cccccccaaa attgcgaggc gaagctttct gtcgtaaatg ggtgccttcc
    19861 tcttaccagt catcgcactc tgctgttccc cgcagctctc tgctcgctcg ggttgaattg
    19921 atccctaacg gcccacttct gccaccgagt agctccccga agatataggc accgcaaccg
    19981 cgcaggcgat cagccccaga tgcacccagg taaaggccag tatcgttcct tcggtcacct
    20041 ggaatgacac ggcatagacg gccgccgaaa ccagcaacgc cagtaacacc ggatcggata
    20101 cgcgcttcag cctccgccgc gcttgccgga ccagggcccc cacaaacagc acaaacgccg
    20161 caaacccaat caccccggta tcatgcaaga tcctcagttc cgtattggcg atccaggggg
    20221 tttccgtgcc gatatcaatc tcggcggttt cccgcataaa aagaagttga aagctggctg
    20281 tgccgttacc gagaagaggg ctcgcgacga aatcatccac ggctgccgca aactggacga
    20341 agcgaaacag tgtgttctcg tcggcggttg ggtcggcaac gtcgagcgtg ctcagccgct
    20401 cggaataacg tggtgcgagt actggtaagc tgaaaatgcc cacgcaaagc gttgccaccg
    20461 cgatggaccg cagcaccttc caattgagca accccatctt gcgggcgcag taggccaaca
    20521 gcgccaggcc gatgaaagtg gcgcccaagt cgcagcggga aaggctcacg gccatcccga
    20581 ccagcgtgat tccgtatccc agcaggtaga ttcgccgctt ctcctgcagg tacatcgcca
    20641 gcatggccac cgaaaaggcc ccgcaatagg cccccaggat gttggcttcg aactgggtgc
    20701 catacgtcgc tgggatggcc ccgtactggc caacctcaac tccgaactcc gtgtcgaaca
    20761 gaaagttcga gtaataggcg atcaccccgt aagcggccgc cagggcgccc acaaccagaa
    20821 aggcgcgaaa ggcccggcga aacctccgct catcatcggc cagcactcgc aagaagaagt
    20881 agggcaagat ggccagcagc tgctgcattg aggactttag cgtctgtggc ggcgccgggg
    20941 acaccaccaa cgaactcaga atattcaaac ccacataagc ggccagcgca taatcggcat
    21001 aaatccacac tgccggctgc tgcgtccgcc gcttcaacag gagcggtatc gccaagcaca
    21061 gaaggccggc ggcaatgtgc tcgggccgca cgttcagccc tccgatgtcc acggagcctc
    21121 gcggaatcgc agccgcgacc acgagaagag caattcctgc ctcggcgctc accgtaagcc
    21181 attgaaacat ccaaaggcca accggcaata atgcggctcc aacgagccac catcgcgccg
    21241 attcgtccgc gatcaacgga gtcaatgcga gcgcgattgc aatcaggaac agaaccggcg
    21301 tcgggtctgg aactgttcta gatggcgaga gactactcat cgcagatctc ggcactgtgc
    21361 cagcggtgcc cacgagtcgg actttctggc ctgaaggcca gggcacgcga gcacgaatcc
    21421 agcggcattt cggggcaagc ccattgggcc gttaaccgta caagacctcg ctctccggcg
    21481 gtttgccgtc agccttgaaa cgcataccca gccattgccc gcagcttttc gtaaatacgc
    21541 cggtacgact ccgacgacca caccgtccgg actgcggtcc tggctttgcc gagtgattca
    21601 atgtcgcgat tgtcctgctc cagaagctcc acctggtgcc cgtaatccgt cagcaaggcc
    21661 acctcggcgg cggtggattg gtcctcaccc ccggggacca aatagcggtt gtggagcatg
    21721 agaactttca tggttctcta cagcgtctcg gatggttgat ggagatggac cgcgccggct
    21781 gcaagccgcg ccgtgggagc gcttgtcgca tgcgcaagcc atgcctgaaa catcagaacc
    21841 ggccagagca gatattgcca gttgcgcgtg ccggaaaggt gttcttccca cttctgccgg
    21901 atcggctgta ctgcgaagaa gcctcctcgg cccaggctct cctgcgagag caggtcttcg
    21961 gcccattccc gcagcgctcc ccggatccac agatcaacgg gcatcgcgaa ccccatcttt
    22021 ggccgctcga tcaactcggg cggaacgtac cgattaagga tctttcgcag cgcccatttc
    22081 gtcttccccc cgcgaatctt gaaacgcagc ggcaaccgcc atgcgtattc aaccacccgg
    22141 tggtccagga tggggactcg cgcttccaga ctgactgcca tgctcgcccg atcgaccttg
    22201 gtcagaatgt catcgggcaa atagttcatc aggtcggtga gcatggccat ttcctgggcc
    22261 gtgggcagcc aactgcagtt ggcaatggcc ttgggcaccg tgccgtgctc gtgcgacccc
    22321 agcacgaggg ccgatggatc cggccacatg gagatcgcat gcaaatagat ggataccggg
    22381 tcttgcgcgg ccagatgttc ggcgagccgg tgaaacttgt gtccgggcga gttcttcagt
    22441 cttcccggaa gcggccacgg ccggagccaa ccatcgatcc gggcgggcgg caccgaacga
    22501 atgagcttcg cggcgccccg ggccgcaggt ccgggcactc gcctgagaat gctccaaagc
    22561 cgcttgacga acgcatagcg cgaatatccg ccgaaaagct cgtcgccgcc gtcaccagaa
    22621 agcgctaccg tcacgtgccg gcgcgccagc ttcgacacca ggtacgtcgg aatctgcgag
    22681 gaatcggcaa acggctcgtc atacatgccc ggcaataatg gcaccacgtc ctgcgcgtcc
    22741 tgcggcgtca gataaagctc ggtatgttcc gtacccaggt gctcggcaat tctcttggca
    22801 tgcctggcct cgttgtaaat gccttcgtga aagccaatcg tgaaggtctt caccgagcgg
    22861 ctgctctgcg actgcatcag ggccacgacc agggacgaat caatgcctcc cgagaggaat
    22921 gcccccaggg gcacatcggc aatcatgcgc aggcggatgg ccgccagcag cttctgatga
    22981 agttcttcga tgacttcggc atcgcctccc tccaccagag attccaggcc ccgtctggcc
    23041 acctccatcc ccgaccagta agcggacatg accggtggtc cctcagcgcc atccatctcc
    23101 agcctgtgtc ccgccggcaa ttggtaaatg ccctcataga tagaatgggg cgcgggaaca
    23161 taggcacatc gcatgtacgc cgccaaagta tcgcggtcga tgccggcctg aaaccccaga
    23221 aactgccgga tggccttcaa ctccgaggcg aagacaaaac taccgtcaac atatccgtaa
    23281 taaagaggtt tgatccccag gcgatcccgc accaggtgga gccgtctttc atggcggtcc
    23341 cacagggcga aggcaaacat gcccacaaag cgcttgaccg ccgcttccag gcccctgcgt
    23401 tctatcgctt ccagcatcac ctcggtatcg gagtggcccc tccaggcgtg ggcgcccagc
    23461 tccgctcgga tctcttcgaa gttgtacacc tcaccattaa agatgatgac gtagcggccc
    23521 gaagctgagg ccatgggctg gtgcccctcg gcggagagat cgatgatgga gagtcgccgg
    23581 aaggcgaggc ccagacccgc accgttatcg tagaacaccc cggagtcgtc aggaccgcga
    23641 tgggccagag tcgcccccat ccggttgagc agttcaacgg gatgttcatc tatggttttt
    23701 cttgtccaga atcctgcaat cccgcacatg ggaacggtct ccggttcatc cggcctggag
    23761 ccaacggcac gcggaaaact tcgcttttct cgagaaaaga gccgactagg cccgcagagt
    23821 ccatcatcga gaaacgaagt tacacattgc aaggattata gacgatgcca ctggtctcga
    23881 atggatcacg ccccttacga tagttcgcat tggccaagac tcgtcggaat ggagcgtgca
    23941 aaccgccgcc tcggacgggc gatacgatca ttgccccggc ttcacatctg ggattgcctg
    24001 aaatcctgct cgatgttgtc gtcacgggct gccggcttgc gcagcctgcg gggccacaga
    24061 gagcgaacgc cagagtatcc gcgcgacagg ataatccgcc gtggatcccg cgaatcccac
    24121 ttctcccact tgtcctgcaa gatgactccg ctgcaagcca gtagcgtgct caacaccagt
    24181 cccgccagga gtatctgcca ggggcgcgga tgccgttttt ccggcacgat ggcggcatcc
    24241 aggactttga ccgtggggat ctccttcgcc tcccggattc gagcgatttc gtattgctgg
    24301 gtgagcatct cgaacacagt ctcgtgaatt ttggattcgc gataacggtc cgcccactct
    24361 actccgagct tcggcaggtt cttcaccgaa gggtagggga aggtcgggtc ctggacgccg
    24421 cccgggtccg cgcggcctga attgatcctt ctaagctggc gttccaattc gcccactcgc
    24481 gcactgagtg taacaacccg gttgttctcc gaagaataga tctgccggag tccttcgagt
    24541 tccgcccgcg cggcgatgag ttctccctgt agccgagcgg ctgattcgac cgtcaccttg
    24601 atctgctctg gtacgtccag cgccatggta ttgctcgaga actggctgaa ccgctgctct
    24661 gcttcctcga gggccttctt ctcgtcggcc aggcgctgtt caatgaacat ccgctcccgg
    24721 cgcgccgaag aagttgagac ccgggtgatc actctgtcca gctcctctac ataggcattc
    24781 gcgagattgg cggccatctg ggcgttcctg tcctgcacga taatcgtgag aacgccgctt
    24841 ttcttgtctt cttcgatctt tgtttttgag tccaggatct tgcgtgcatc ttcgttgtat
    24901 cgcttcccgt atctttggcg gaggtgaaaa cgctcgacga ggctgtcctg gatcgttcga
    24961 cttcccagca ctttgacaaa cagtgctcca gaatttttcc ctcccagtag atccccggca
    25021 agggaaccta atccgccgcc catgtttggg agcccggaca ttttcgaaag cattggaaat
    25081 acttgtgaac tcaagctgga tgacgcgctg tcaggcggca tcaactgcgc ggtggcttcg
    25141 aagtgacaca ttctcgtggc gagaagaatc gaaataccga agccgatcgc cacccagagg
    25201 ctaatcctgc ggcggcggtt ccagaggcgt tccagggtat ccggccagct tggccatttt
    25261 cgaggggatt cagaaaggtc gcggtcttct cgagcatcct ggatctcgtt ttcgatcttg
    25321 ctcgacgaca tgtgcaggcc tccggctgat ccgccgcggg acattcagcc tttacaatct
    25381 tcaagaccgc tggacccatc ccgttcgccc aaacaggatg agatgccccc aagttgcaag
    25441 cagacattat agtccgatga gcgcagaaaa cagctattcc cgcgatgtca gaggcaactt
    25501 ttcgttccaa gcgcagatca cagacatctg aggcctgcgc ggcggatccc ggtcaatgcc
    25561 cacgcaagtg ggcgggaggc ggtgtacaat gcttctcgct ttcgaattaa ggattggggt
    25621 gtagatttga ttccgcgggc tgccggagcg gctcgcgttt tttctggaga gaatttgcgc
    25681 caacgctctg ctttcacctt tcagactctt cggatatttt tggcggctct gattgcgttg
    25741 tgctcctgtg caattcatcc gccagcggcc gtggctcagc aggaagacac atcgctctca
    25801 gccgaggcca ttatccagat cctgcaggaa aaccccgaca ttctcgccga ggcgaagtcg
    25861 cagatcgtgg ccgctttgcg ggaacggggc ctggccgtct ccgaatcaga tatcaccgat
    25921 gagcgtctct tctcccaaat tcgttctgac gatcgagtgc gactggtgat gagcgatgag
    25981 ctgaaacagc gtggctacgg cctcgagccg gaagcaaaga cgacgcctca accgcaaaag
    26041 ccaggcctgg taccggcgat gcccgaaacg aagccggaag cagtcccatc caagccaggc
    26101 gcgccgcgca cgcccggcgc cgagccaaaa gaagcggtta agggtaagga taacgaagcg
    26161 ggccgccgac cctcgcagag ccgctatccc tatcgcaatc tgcccgcgct gcgcgagctg
    26221 tacatgcctt ccgtagtagg cgtggagaac ctggaacgat tcggcgcggc gctgtttcgc
    26281 aattccgccg tcgccgcgga aaagcccgcg ccggatctcc ccgccggcgt ggattacgta
    26341 ctcggctacg gtgatgagct cgtcatccaa tactggggca gcgtgtccca gcggctgcaa
    26401 gtaagcgtgg atcggcaggg ccgcctcacg atacctgaag ccgggccgat catggtcgcg
    26461 gggcggactc ttgccgaagc cgagcagctc atccagaagg cgctcacacg gcagtatcgc
    26521 aacatctctg tggatgtcac cctcgcgaaa cttagaatgc tgcggatcta cgttgtcggg
    26581 gacgtgaaaa accccggtgc ctacgagatg agctcactct cgaccgcgct gaatgccgtt
    26641 ctcatcgccg gtggcccgac cgacgtggga tcgttccgca cggtcaaaca cttccgcgca
    26701 aagaaacttc tggaggagat agacctctac gattttctgt tgagaggact cggtgcagcc
    26761 gaggcgcaca tggagtctgg cgacagcatt ctggtgccgc cggttggccc gcaagtgacc
    26821 gttgcgggga tggtccgccg cccggcgatc tatgagctcc ttcacgaaga tacgctcggc
    26881 cacgtgctcg aaacggctgg cggcatcctg gtcaccgggg aactgaacaa gattcgcgtg
    26941 gaacggattg aggcccatga gcgcagggag gttctgagca ttgaactgcc ggagaagcct
    27001 ggcgcggctg aagaggcctt caagaaattc gccatgcagg acggcgacat cgtcaccatt
    27061 ctgcccatca cgccgtacgg gaattggacc atctacctga ccgggcacgt cgcgagaccc
    27121 gggaagtatc cctaccacga cggggtcacc gtggcggaca tgatcgcttc ttatcaagat
    27181 ctgctgccgg agccggcaga ccgcgcggag atcgtgcgcc tgcacgctcc ggacaaccgg
    27241 ccgtacgtga ttccattcag cctccgcgac gtcctcgaaa agcgtgcccc cgcgcccgcg
    27301 ctaaacccgc tggatactgt gcgcgtatac ggccgctacg aagccgacgc gccgaaggtg
    27361 gccatctacg gcgaagtgat tcgcccggga gaatatccgc tctccgataa aatgacggcg
    27421 acggacctgg tgcgcctggc gggcggcttc aagcgcagcg cgtacgccga ttctgcggac
    27481 ctcgccagct attccattgt gggaggcgag caagtcgagt tggagcaccg cgccattcac
    27541 atcgcacgcg cgatggtcgg cgaacccaac accgacgcgg tgctcaagcc gggggacgtt
    27601 ctgaccatca atcaactcgg cggttggcgc aacattggtg gcgcgatcat tctttcggga
    27661 gaggttgtgc acccgggccg ctacggtatc gtcgcaggcg aaaaaatcag ctccgtcatc
    27721 aagcgcgccg gcggctttcg gcctgaagcc tatccgaatg cctcgattct cgagcgccag
    27781 gaggtgcgcg aagtctccgc aagaaaccgt gacgagctca tccagaatct ccaggcccag
    27841 gccgtgggtg gaacaaccag ggcggagagc atcatcgtca ctcgccagcg ccaacagttg
    27901 atcgatcgct tgaaacagat tcagcccaat ggccggcttg tcattcatat cagtgcggat
    27961 gtcgccaagt gggagaacac tctggcggat attagtgtgc ggcccggtga tacgctgacc
    28021 attccgaaaa aaccgaattt cgtcatggtt acgggacagg tttacaaccc gacggccatc
    28081 tccttttttc caggcaagcg cgctggttgg tatctgaaac agacaggcgg ccccacgcga
    28141 atcgcggata agaaagccat cttcgtcgtc cgctcgaacg gttccgtggt tggccgcaac
    28201 tcaggcgagt ggtgggcgac caagacctcg catgtcgtct tgctgcccgg cgacaccgtc
    28261 tatgtgccgg aaaacatcgc cggcggtgaa aagctgaaga ccttcgcaat gatggcgcag
    28321 atcctatccg gcttggccgt ggccgcccgc gtcgccataa gcttctaagg aacatcctgt
    28381 ccattccggc tggaggcatc gggttgcgcc atcgcgcttg ccggagagct gatcgccggt
    28441 tttacttacc gtagggttgt tcgatcgaga agcggcctgg ccgcgtttgt tccgcactca
    28501 gactctccgc acggaggccg tctctctggt tgcaaggtat tcttccagcc cctctctcca
    28561 gggacggaat acgttgaggc cttcccgctt gagcgccgca ttttccagta cggaatatct
    28621 gggccgtcga actttggctg ggaattctcc gcgccgcgca cgctccagcc gcacccgcgt
    28681 gccggtgatg tcgaaaatgg cccgggcaaa ttcataccag gagcaactgc cctcggctgt
    28741 cgcgtggtat aggccgtaac cggatgtctt gctcagcgcc gtcaattgcc gggccactcc
    28801 aaccgtcgga gtgggagtta caatttcatc gtccaccact cggatttcct cttgctctct
    28861 cgagagcttc agcatccttt cgacgaaatt cagtccatct tttgatcggc acgggtttcg
    28921 cccatagatg ccgcagacgc gcaccacgaa gtgacgtgga ttcgcagtcc gcacgaagaa
    28981 ctcaccgctg agcttggtat tcccatacac attcagcggg cacggaacat ccagttccgt
    29041 atagggcgta ccctttgctc cgtcgaaaac gtagtctgtg ctgatgtgca cgatggcggc
    29101 cccgtggacg cagcaggctt cggcgacatt gcgccctccc agggcattga cttgaaatgc
    29161 gagcacggga tcggtctcgc accgctctac attcgaaaag gcggccgcgt tgatcaccat
    29221 ctcaggtcgc gtcttctcga atgcgagctt cacggattcc ggcgaagtta tctcgacgtc
    29281 ctggtgagtg agggcggaaa cctcgtgccc ctcggcggca aacgcagcac tgacgtcgct
    29341 acccagctgg ccattggcgc ccagcaccac cactcgtgtc acggaatgcc gcctaggcgt
    29401 aggtttcttt cgagaggaac agccatttga tgttttccgg ctccagcatc caccgcaccg
    29461 tcttcaagag gcttgcagtc agcgtccgaa cgacccgatt cgcgcaatgc gccagggcaa
    29521 agtcaagtct aagccttatc gaccgaaggc ttgggttgat aagggattct atcggagagc
    29581 gacgacgcca gccattgtgc caacgtctgc gcgttccgat cacgctccga taggaggccg
    29641 cgctccggat cgataggcca ataaatgcca atctccggat cgccaaggta gatactgctt
    29701 tctccttgcg ggttgtagat tcccgtgcac ttgtattgca cttcgcactc gtcggtgaga
    29761 gcgcagaagc cgcgcgcgaa gccataggga gcccatacct gcttcttgtt ctccgcagaa
    29821 acctccatgc ccatccattt ccccagcgtc ggggaaccct ttcggacgtc taccgccacc
    29881 aagaaagcgg cgccgctcgt gacgcgcatc agtttgccca ttgggggatc ccattgaaaa
    29941 tggagcccgc gaagcacccc cttggctgac cgcgaatgat tgtcctgtac gaattccgtc
    30001 ggcaaaccta tttcgcggaa ccggtcggcg cgaaatgtct ccatgaagaa ccctctagaa
    30061 tcgccaacaa cctgcggaat gatgacgaat acttgatcca agtgaattga ttcaatctgg
    30121 aaagacatca gaagcccata tctccctctc ggactatcct acgcctcccc tggatgcgga
    30181 agctcctgaa gctcgcggcc aacccacaac aggtccgtag gtctcactcc atgctgattg
    30241 gacgaattcc agtgagactt gtcgatgggt agtcggcaaa gcttctaatg gatgccgaat
    30301 cgacggcggg ctctcgagtt cttacgggca cgggactcgc cctcagtagc cagaacccct
    30361 tagcacttcc ggaatggttt tcaacaatat tttcacgtcg aagaggaggt tccaattgtt
    30421 gatgtactcc aaatcgagca tcacgttcgt ttcaaacgac gcgtcctgcc gtgcgctcac
    30481 ctgccacaaa ccggtgatcc ctggcaaaac atcgaggcgc cggaggtcct ccaggcggta
    30541 ttggtcaaag tcctcttgag gatgcggccg cggcccgacc aggctcattt ctcctctgag
    30601 tacgttcaag agttggggaa attcatcaat gctgaacctt cgcagaaatc gccctactcg
    30661 agtgatgcgc ggatcgttgg cgatcttgaa ggtggctcca ttgcgctcgt tcaagtggag
    30721 gagttcttcc ttctgtgcct cggcgtcggc caccatcgtg cgaagcttgt aacattggaa
    30781 cttgcggcct ttgtgcccca cgcgtcgggc cgaatggacg attggcccgg acgagtccag
    30841 cttgatggcc agcgcagcga ggagcatcac tggcgcgccg aggatcgcga gcacgccgcc
    30901 gaaagtaagg tctatgaggc gcttgaggaa caatcccacg gcgggtatcg gctgtcggtt
    30961 cagctccaac acgggcaatc ccccgatgtg gtggatcggg gcgttccacc ccagcccgtc
    31021 gtaaagttca gggacaacct tcacattcag ttgttggcgg caagcttctg ccaccacgcg
    31081 cttcaccagt tctcgctcgg aggggatcgt gacgaagatt tcatcgacaa actgtgcgcg
    31141 ggccacccat ggcagatcct cgattctacc caggtggcgc gggtcgtccc cgcggtcacc
    31201 gtctaagaac ccgaggacct tgtatccgag ctgcttgttt tcctccagat atcgtgcaag
    31261 ttgttgcgcc acgctcccgg atccgatgat gagcgtgttc ttcgcgctgc tcccgtgtgc
    31321 cacccgccgg gtcacaagcc gccgcttcca cgctcgccag gctaccagcg tcaccaaact
    31381 cagcgccgtg ctcgtcccca gcacctctcg agatacgatc tttgcattcg aaataaagat
    31441 ggaggccgtc agcacgagag tggcgaagca tacagcgcgg attaccgccc agctctcctg
    31501 tttcatcgac cgtccgcgcg gcgtgcggta tagatcctgg ctctggcaga acaacacgat
    31561 caggatcgtt tggagcagaa ggaaactggc gtactgccct acggggaacc cgggcgcaag
    31621 cttcggccac gcgtgccaca ggccaacccc caggggaatc gcggcaaagt ggacagagaa
    31681 tgccaacaat ccattcgtac agatgagcaa aacatccacc agcacgtatc cgaagtgcac
    31741 ccagttgcgc atccgtggtg atggattctc ccgcttctgg gccgagtgat tcccgggcac
    31801 ttggcgcagc cacttcccgg tgtcgggggg tcgctccgtc tcggggatga ccaggggggc
    31861 tgggtctact gtggcacccg cagtggcaaa aagcagccca ctgacgcctg tctgttgcga
    31921 gcttttcatc ggtatcccca accatcctgt agggtgatat ttttggccgt gcgattcgcc
    31981 ggcattggtt cgaaggcagc cgaaccttcc atcctcagtt tgatgcggaa acaccattcg
    32041 ctcgtcgagc aagacacagt agcgaaacca aacctcggct ccctgcgcgc aagtttgcgc
    32101 ccagccaccg actgatttgt gaacaagtcg gttgagatcc ccggagcgct atccgctcgc
    32161 cctctcacgg ttttgcctct gcatcggaag gcgtcgacgt cccccgtcct tttttcctcc
    32221 gccagccttc cggagagaaa aggacttgta gcgatgaagt gatgttgtcc gtaggagcgg
    32281 gccgcatcac agggatgaac catcgctcgt gctgtacgcg gatcgaaagg gaaaggtccc
    32341 ttcgcatctg cacttcgccg cctacgccga aatcgctcaa ggtaccgccc ccagggatat
    32401 attgttgact gattttttca tgccggaaag tcgtttgcag ccggctgtga gcattgagcc
    32461 aatacgtacc ccaggcctgc gctccctgcc catcccggcc tatccagctg ccgatcaggt
    32521 ttccgtcgtt ctgatatccg cttctccacg tatcattaaa ataatagaac ccgtggccca
    32581 gagcgcctcc caacgggttg tccgtataga cgccttcgac ccgtaagtcc aatttcgcaa
    32641 tcccggggaa gtgggaaaga tagagccccg catgccacgc agaacggtcg aaataaccga
    32701 tgggcgaaaa ctcgtcatcc gtgaaaccgt ccccgtagaa cgtcagccaa ttgcgcagga
    32761 gcggaagacg gtaactgaaa tccacgcccg atcgtcggtc gcctgctttg agtgggctgg
    32821 ccgccgcctg gttgccaccg gaaaaaaggc tgcgcacgag agagcgtgag gtcaggggat
    32881 accccgggcc tccgtagatc gttgtccgga agacaccgaa ttcaaggttc ggtgtgggct
    32941 tgaagctgag tttttgaccg tgaataaaag gctgttgggc taaggatacg ccggactgtc
    33001 cgttgaatcc tgtaggggaa aaaacaaatt cgtagcccga gagttggcct aggaagaatt
    33061 ccatccgcat ggggccaagc cagcccagga agctgggcag cttgaaggga gtgacgcggt
    33121 tcacccgaaa catgttgatc ggtctggcgt tgtcgctgaa catcatcgga ccgccctggc
    33181 cgggtcccca ccaaagactc tgccgcccaa agctgagctc ccattcggac aacgtcaatc
    33241 caacgtaggc gtccagcaat tgaaattggt tggccgcagc ccgcggagtg cccgggggcg
    33301 tttgtggaag aatatccact acttgaatcg tttggcgcgc ggtcagcggc aacgccgggg
    33361 cgctgggagc cgtctgccac tcgccgcgca catacgcgac ccagggccct tgcgtggtat
    33421 aggccgaaaa gccgtttatc gtattccacc cttctgcatg gggacgcccg aagtcgttga
    33481 tcaccgtctg tccgaagtga tacccgtcgc tcagcggggt tcccgaaata tattctgtgc
    33541 gcgaatacac cgattccacg cggaacgtac cgcggccgcc gctctcgctc ccttccatct
    33601 ccgtcctgaa ttcccgctcc agggctttta gtagctcgac tgtttcttcg gcgtcggttc
    33661 cttcgtctcc cagtttttcc tgagcttcaa cgagcagccg cgcgcactcg cgccgcgtcc
    33721 acggccgcat cccggcaaac ccgctgtcaa cgaattcgag ccccgcgagg cggtccagcg
    33781 cgggatacac ccagctgtcc agcggcacat agggagaagc tgcgctccgc gcgtccttcg
    33841 attccttcac gctcagggcc tcctccgacg actcccacgg cgtgtccccc actgccggat
    33901 catggcgccg gcggtaaata tttcttgcta cgagctcccc gatcacgctt cccacaagga
    33961 cgtccgaagg aaaatggtcg cgcgacttca cgcgtgaaag ggtgaccgcc gaagccgccc
    34021 cgtaggccag cagtttcgtc aggggattgg ggtattccct tgcgatgact cccgccaccg
    34081 cccaggcggc ggccgcgtgc tcggacggaa aggaggttcc gccgttcagg aatttccccc
    34141 cgccgtcacc ctggaagggc cggctgcgtt ctgtcgaata cttcatgacc tcaacgacga
    34201 ccaaactgtt aatcaccgcc tctcccgcga gaaatcctgt ctccctgcgg tggtcctcat
    34261 gggtccactt cccccacaga taaaacccgc cgccaacgcc gaccagcgat gccacgcctg
    34321 cagtgccgag cgtccggtag tgttgaattg tgtttggatt gcgcgagagg tgcccgctca
    34381 attgtcggtc cgtcagaagg agtccagccg tcacgccgcc caggggcaca agccacgtgg
    34441 tatcggcaaa gcggatcctt gacggactcg tccagacttg cttctgatcg ttaaagaaat
    34501 ccttccaaat cccctcccgg ggctctggag ggttcttgcg cgtggtgtcg gctggggtgg
    34561 atttttctgt ttttgaagct ggctttgcgc tctcttgatt gcttcccgca ctcagactct
    34621 gggttttgtc cgcctggcgc tcggccgcat ccggcttttc ctgtccaaga atctcgctcg
    34681 tccccagaaa tatgcaaaac agacagagcc ctgcggcgag ccctttcccc atcctgcctc
    34741 ctgcggatct actccctgct tcttcagact gcaccggatc ggctagtccc aggtgcaatc
    34801 tggaatgacc ggggccactc tccatgacag ggagagaaaa atatattacc tcaccacggc
    34861 gatgcgcagc gaaataactt tgaggttgtt cgggattgat tatgttcgct gacgaattgg
    34921 gtctcgtggt gtgcaattcg gactcgcgaa agatacacaa attctacgag cggtagagag
    34981 tgtccgattt gatcttgagg tttctcagga ttcagcgctt gccgtacctc cctagggcta
    35041 tggttggcgc gccatccgta gccttggcga aggatggcgg agaggggggg attcgaaccc
    35101 ccggtagagg ttttggcccc tacaacggtt tagcaaaccg ccgccttcag cctctcggcc
    35161 acctctccgc ggcgcgtcca ggaatcggtt ttctccccgg accagccgag tataacatcg
    35221 aaaccctggt ccacggcgcg cggcacgctt tccgccggcg aaggaacggc gtcagggatc
    35281 ttgcggaatc ggcctcagag tctgtgccgc aaaattcttc acagcacggg gtcctgcgga
    35341 gtcgcggccc gattctcccg cgtcacgtga atcgctccgg tctggtccgt gcaaaagctc
    35401 aacaagccgt agcgcccggc ccgtgccgtc agcgtgtagg cgtgcacggt tccatctttt
    35461 tctggcgcgc ccggcgtgta ctgcaccgtg tacccgccgc gcgaagcggc ctgcgccgcg
    35521 gccttcgcct ttgcgccgag cggctcgagc gattccggat aatggccttc ggccgaggcg
    35581 aacaacgaaa gctgggattg caccacacgc agcgcgtcca cggtcgccgc ttcggcctgc
    35641 tgcggcgcgc cggttgcgga atattcgggc aggacgtacc actcgaacgc gtacaatccc
    35701 gctgcaatga ccgcaatcac cgccgcccag ataagcagca gccgcggcag gctgcgcttt
    35761 ttcaccggct ccgcggccag agccgtctgt gcgagctgcg ccaggtctgc tccgcaccgc
    35821 gggcagatct ccgttgcctg gttgatctcg gcttcgcaat ccggacacgt gtacatgttc
    35881 ccgccaaccc ggttccgtag tgtagcgcgt cctgtgcgcc gcggggccag ggccatcccg
    35941 agatcgagcg aggtcgagtg acgacggcgg tgctaagctc cacgttgctg tccatattcg
    36001 gaggaagctc gatgaaagtg tcacacgtgc attgccgcgt gcgggacttg ccggctgcgg
    36061 cccgctggtt tgaacaggtg tggcaggtcg cccccgtgtt tcacaacgag cggatggtct
    36121 ggctgagctt tggcgagttt ggcgtcattc tggacgccgc gcctgctgat agcgtggtta
    36181 cactcagctt cgatagtgag gattgcgacg cagactatcg cactgtcacg ggccgcggcg
    36241 cggagacgat cgaggcgccg caagatcgcc cgtggggcgc acgcgccgcc taccttaagg
    36301 gaccgggcgg gctaacggtc gagatcgaac agctactgaa acgcgcctag tagggcggaa
    36361 ccgtggtggg catcgctgga gtggtgcact gcctggcgat tctgcgttat tgctgatttg
    36421 ccgccgagac acccgccgga aatgctttcc aaaatgaccg attgcccgta aaatatctcc
    36481 gcggtatcag cgaccgggtg gcgttgcacc cgtcagggtg cccgcaagtt gagtccggct
    36541 cccgaaggag tccgtcctcg ccgcgctggg gatctgccga ttcgagaaac tttttgggtt
    36601 tccgtgcgta ttatggggat agaacaggga aaaccggcgt ctaatttttg cgtacgattc
    36661 cgtgtggatg tgcataagta gttgaaaggg aatgagatga agacgtggca taaggttggt
    36721 atcggggtgg gtgcggtcgc cgtcctcggc gggatcgttc tctacagcgt caacaaggcg
    36781 aaccagggtg tggtcaccgt gcagacttcc aaggtcgcca cccaggactt ggtctccctg
    36841 gtcaccgcct cgggcgaaat caagccgacc acctacacga acatctatgc cgagggattc
    36901 ggcaagatca ccgcgattgt cgtcaaggaa ggccagcacg tgaagaaagg cgataccctg
    36961 ctcctgcagg agagtatcca gcaaaatgcc gatgtgcagg cccagacagc cgctctctcc
    37021 tcctccgatg ccggcatcct ctcttccgat gcgagctaca agtcggcgca gtcggatctc
    37081 cagcagcgcc gctcggatct gttgcgcgcc aagctcgatt gggagcgcgg gcagggtctc
    37141 tacaaggacg ggctcatccc caagtccgac tacgacactc gcaaagcggc ttatgacggc
    37201 gccgttgcgg ccgagacctc cagcgtttcg cgggtgatgc agttgcaggc ggaggttgat
    37261 cgctcgcgcg cgcagcagca gcagagccgt gccatgctca cgcgcacgcg cgacgttctc
    37321 cgcaagacca attacacctc ccccatcaat ggcatcgtca gctatcttcc ggtgcgcctc
    37381 ggtgaaaacg tcgtccccgg cattcagaac accaccggca gcttcctcat gaccatttcg
    37441 gatatgtccg tggtgacgtc ggaagtgaag gtggatgaaa ccgacatcgt caacgtcaag
    37501 atgggacagg aagccgacgt gacgattgac gccgtgcccg gcaaggtctt caaaggccac
    37561 gtcacggaaa tcggttcgca ggccaccctg cgcagcagcg gcctggcgac cacgcagtcc
    37621 accacgagca cgcaggaagc caaggacttc aaagtcgtca ttacgctgga aaagcccccg
    37681 gacaacgtgc tcccgggtct ctcctccacc gcgaaaatca agaccgccga acggaaaagc
    37741 gtcgtggccg tgcccatcca ggcgctggcc gtgcgcaccc gcaaggatct ggatgaagcc
    37801 gctcagcgcg ccaagaaggc caagaacacc ggcgaaagcg tgacgctggc cgcgccatcc
    37861 cccgcgccgg ccggcacgga cccgaaaaag gaagaaatcc agggtgtctt catcgtccgc
    37921 ggcaagaaag cggtcttcac tcgcgtcgaa accggcatca ccggcgtcac cgacattgag
    37981 gtcaagagcg gattgaagcc gggcgatgag atcgtgaccg gcagctacaa ggcgctgcgc
    38041 actttgaaac ccgaggctga cgtcaaggtc gacaattcgg cgcccaagaa ggcggaagac
    38101 cagcaaagct gacgcctgcg ggctgaagcc ggttcgcaat tatcagtaaa gcggaatatc
    38161 ccgaggagca tgtgaatgcc cgccgaaaca cagaccgctg attctttccg gcaagacctg
    38221 gtttcttccg gcgtggtcat caagacggag gatttgacca aggtctacga gatgggcgcg
    38281 gagaaagtcc acgccctgcg cggcataagc ctggagatcc acaagggcga gtacgtcgcc
    38341 atcatgggcc cgtccggctc cggcaaatcc acgctgatga acctgattgg ctgcctcgat
    38401 tcgcccagct ccggcagata ctggcttgcc gggcgcctcg tgagcgagct cgacgacgac
    38461 gagttggcct acattcgcaa caaggatatc ggcttcgtct tccagacctt caacctgctg
    38521 cctcgcgcca ccgcgctgca caatgtcgag ctgcctctca tttacaacgg cacgcccgcc
    38581 gcggagcgca tcgagcgcgc gaaaaaagcg atcgagatcg tggacctgac cgaccgcatg
    38641 caccacaggc cgaacgagct ttccggcggg cagcgccagc gcgtcgccat cgcccgcgcg
    38701 ctggtcaatt cgccttcaat cgtcctcgcc gacgagccga ccggcaacct cgattcacag
    38761 accggcgaag agatcatggg cgtcttggcg cggctgcacc agcagggcaa caccatcatt
    38821 ctcgtcaccc acgaacacga catcgcccag catgcccacc gcagcatctt catcaaggac
    38881 ggcagcatct tctccgacca gcccaccaag aagtaactga acaaaaagca aggcattcgc
    38941 cgcatcctcg aatcccgtcc cggcgactga ccggtagtgg cgaaagctgc ccccccaccc
    39001 atcccaccgt cgccctgccc ggccacgtcg gcctgattgg ctgcgcacat atcgaatatc
    39061 aatatacagc ttgacgaaaa acgatattca gattatcgtt attcaataat acgatgcgac
    39121 tcttcctgaa
//
LOCUS       DASYVO010000474        17085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_8119, whole genome shotgun sequence.
ACCESSION   DASYVO010000474 DASYVO010000000
VERSION     DASYVO010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 17085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17085
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8119"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..709)
                     /locus_tag="VGR03_16225"
     CDS             complement(32..709)
                     /locus_tag="VGR03_16225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1995880.1"
                     /translation="MPLPRWALALADNNGRSGSTDAALASVISLSHLDERALVAEAQT
                     GSRAAFEELVRRFDRDVLRLALNLMKRTEDARDVYQEAFLKVYRNLHRFRFECSFYTW
                     LYRIVTNVCLDHLRRRQARPEDQAPELNSGLHEEGITDFFERQREHRPTLDPERTFIG
                     KEIKARIATAMERLSPRERIVFEMKHYQGLKLRAIGDALGTTEETVKNSLFRATRKLR
                     HELGGLR"
     gene            complement(945..4418)
                     /locus_tag="VGR03_16230"
     CDS             complement(945..4418)
                     /locus_tag="VGR03_16230"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEV1995881.1"
                     /translation="MTGDRLTSSDKRALLLWVFAGILGALFAYKYFFQAFPEASVNFQ
                     VSREEALSRAQKFVTGLGENVSGYESTIVFNVDDNAKVYLERQLGLQEANRLMSSELN
                     IWFWDVRFFKPQQEEEFSVRVSPAGQIISYDHKIEESRAGASLDRATAQSAAQTYLNA
                     KLGLDLSGWDLLPEEANSNKRPNRLDWAFTWEKHGFRAKHAPYRLQVTLQGDRIGGSG
                     EFLRVPEAWERGYQRLRSGNNTLALIFTIPYLLLLGTAVWLAIQLTKSGETAWSAALK
                     LGALATVMLFLQSLNDWPLWGAGYDTKDSYSTFIFLQIGRALLLAAASALTITLVLPA
                     AEPLYRKSQPGQLRLGQIFTRRGLRSKEFFSSSVVGLCLAAAHIGFIVAFYIVATRLG
                     AWAPQELNYSDSVNTAFPWISGIAIGLLASMNEEFTFRLFAIPFFMRFTRSRWVAVIV
                     PAFLWSFLHSNYPQEPAYIRGIEIGIIGIVAGIVMLRWGILATLIWHYTVDASLVGLL
                     LIRSNSLYFKISGMVVAAAALAPLVFACISYLTRGGFESDEDLLNRSAGSPKLDLTSE
                     PVSETVEVTSRRYDALAPGMIAFLSVCLLAGGALLWRLKPQSVGDYLKLSVDARSARA
                     RADQVMRQRGLDPNSFHHATIFIDTPHPRSNVDFPDPATNAFTNEYLRERIGAAGVNA
                     IYKERVPGALWHVRYFRDSQPEEFSIILTPDGSVQSVRHTLAEEAPGAALTKEEAAAR
                     AKKFLAEEKKIDLKAWTLVESNSDKKPHRIDHTLTWQENEPLDAGQYTTSSVTDHAHA
                     RIDVLLLGDEIASYRTYIKIPEEWRRKQEERTLFRIIVGYVVPILFFLGLGLTALIVF
                     LKNLKSETARSIPWRRIGFWSVWGLCGYLVVFLLGNRVPSFLNAYETAIPLKLMFGGI
                     AIGALFGALFNFGGIALLFGMAWYYATRAFEVSRLPAWAEMPANYYRDALWMGLGGAA
                     GLLGFERLLVAASTHWPTVHRSLEASFGQDFDALLPSASILGGAVVRGLFLTGLVALI
                     ASFVAAHVRQPALRIVLLLVGSLSLVSGGWGSPRDLAVEFLAQLILLSVLVFGVRYVM
                     RFNILGCFLIVAGTSLFGGAAELLSQPDSFYRANGYALLLALVLLFAWPLVAWRMGDS
                     ARTLGALDSGL"
     gene            4526..6706
                     /gene="recG"
                     /locus_tag="VGR03_16235"
     CDS             4526..6706
                     /gene="recG"
                     /locus_tag="VGR03_16235"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV1995882.1"
                     /translation="MSSAVLRPRLQLAVLWPHVLMAMNLSTEVRMIKGVGPQRAELLA
                     QRGIYTLEDLLGYLPFRYEDRIHFSKVKDIQPNGTYTIRARVLSGQAVRSMRGGRDAI
                     YHLLVQDDAGGSLPCKFFHGGYLEGRLKPGQLLILHGKAEIDRLRPARLEMINPQIEV
                     LSNEEMDSTEVGRIVPIYEAIGTFGSRQIRRAMYAAVQLIDSRMPDVLPEGLRARLGY
                     PSRGEALIHTHFPEPGESLDALNTFRSTAQQRLIFEEFFLYQLSLALDRKADRKENAI
                     AFCVREDAVREALKRILPFKPTAAQKRVLAEIAADLEKPAPMNRLLQGDVGSGKTIIA
                     LQAAVIAIENGTQAALMAPTEILAVQHFLSARRLLGKAGYRVELLISGLKASEKAAAL
                     DRIRSGEAQLVIGTHALIEDNVEFARLGFVAIDEQHRFGVLQRKRLMDKAAAHGHAPH
                     VLVLTATPIPRTLSLTLYGDLDVSILDELPPGRTPIGTRMTTEQHLPGVWESLRREVA
                     AGHQGYVVYPVIEESKLELKAAVEEYQRLSREVFPKLKLGLLHGRLSSEEKEEVMQRF
                     RKNEAQILVATTVVEVGVDVPNATVMVIEHAERFGLSQLHQLRGRIGRGAQKSHCILV
                     APFRMTDEARARLETMVRTSNGFEIAETDLLLRGPGEFFGTRQSGDLGFHIANPLRDR
                     ELLELARREAFALAADSAQHETMQRLLRQLPPEWQRRYHLARIG"
     gene            6868..7362
                     /locus_tag="VGR03_16240"
     CDS             6868..7362
                     /locus_tag="VGR03_16240"
                     /inference="COORDINATES: protein motif:HMM:NF024671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin SocA
                     domain-containing protein"
                     /protein_id="HEV1995883.1"
                     /translation="MNIVSTGLSAKTVANYFLDLASRDGVPIDPLKLQKLIYLAHGWS
                     LVLLKKPLIKEPFEAWNYGPVVPSLYKAFQKFGASPITEHAPKEPFESHYELDAQTKS
                     LLDSVWETYKKLNSIQLSMFTHEPGYAWDLARRGGRMSPWGSPAIPNELIANEFIRRQ
                     QQAE"
     gene            7369..7770
                     /locus_tag="VGR03_16245"
     CDS             7369..7770
                     /locus_tag="VGR03_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995884.1"
                     /translation="MTDQPAAKKPEKSLKIPDPRIEELKVSAADSISLADAAQEYRGF
                     LSRNKSALTRTWWDWLLTYVLKLILFGFVLAINIWWTRNVLKMVWLSGSQGSHFHLDN
                     NVLITLVSTSIASFLGLVAIVTKNLFPDSSK"
     gene            complement(7879..8154)
                     /locus_tag="VGR03_16250"
     CDS             complement(7879..8154)
                     /locus_tag="VGR03_16250"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1995885.1"
                     /translation="MFHLIWYVLIGLISGVIAKSVMHVHITIFWTIVLGIIGSIIGGG
                     LTHMLSRPTNERYHPAGLIFSTLGAMLVLFICYKLNIHFPEVNLSTR"
     gene            complement(8542..9081)
                     /locus_tag="VGR03_16255"
     CDS             complement(8542..9081)
                     /locus_tag="VGR03_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995886.1"
                     /translation="MRKSVFGRAFLPPLVLAFSSGCGNVAANLAQQNDPPQAPQQTQA
                     GQLAHVFTLATSSPATYSNPSGALELMPSTSLTFQSQSSGFLTVSFGARGSVAPSGSQ
                     IIPIVFIECHIDGQACQPDTNTVEFLYPQFCCDTRSFHWVAQNISAGSHTVDILWGMG
                     NPTSAIVTNRSLIVETATP"
     gene            9241..9840
                     /gene="rsmD"
                     /locus_tag="VGR03_16260"
     CDS             9241..9840
                     /gene="rsmD"
                     /locus_tag="VGR03_16260"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV1995887.1"
                     /translation="MRVIAGTYRSRILKSLKGLALRPTSDRLRETLFNVLAPNIAGSR
                     FVDLFAGTGAIGIEALSRGAAEVVFIENHAPAATLVRRNLESLGISTGVTVLAVDALR
                     GLAMLASRKNQAEPGFDYVFLDPPYAAAEDYSRVLEFLGSADILAPGGMVVAEHRRNF
                     DLPEEPGALRRFRVLRQGDAAFSFYCRRGEAGGENNSAE"
     gene            9938..11650
                     /locus_tag="VGR03_16265"
     CDS             9938..11650
                     /locus_tag="VGR03_16265"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEV1995888.1"
                     /translation="MSLNQASNAFDLLGQSGEPAAPVDLQTGEGPKLAVGWGSFHQGI
                     GSSIAALFSRTVVPKNSAVGDFFKDCWIERRIPRRALIAAALWHFVFVLMPFPQLPAA
                     ARHSSALDNTELTWSGPINDFPLMEIKSPKAKPSPRGEPAKPLPREGADAFHPRQRIF
                     TDPVRPTHPRQTLINSAAALEPPKILPNLPNIVQFQQMAGPARPRLQICQETLSKLRP
                     RELRKVKLTSAPAPDLPTLDQKLSDIALSMLPNAPARPKLELNAGAAPRVAPRAQAGE
                     VEPAPEVGSVQTGLANGNAATFIALSATPGPPAPVVQPPQGNLAARVSISPEGRQPGV
                     PGGLPNGMPGANGGAGGGPESSGGTATGNGGGKNGKDVSITGGHPPANNGISGLGGSA
                     KISAPIPHALILKPDPHTKIDDAPERTGPPNFEALPAGALPEQIFASKKIYKMLVNMP
                     NLNSATGSWILNFSELRVNSEGPRLASSDVSAPGPLRKIDPKYPPTLIKERVEGEVVL
                     YAVIRRDGSVDSIQLVRGIDEQLDANAMEALSQWKFRPATKQGTPVELEAIVHIPFNP
                     PAER"
     gene            complement(11676..12605)
                     /locus_tag="VGR03_16270"
     CDS             complement(11676..12605)
                     /locus_tag="VGR03_16270"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEV1995889.1"
                     /translation="MTEQYSWPKIAVVGAGAVGGYFGGLLARAGAPVAMVGRPAFVEA
                     VKKCGLFLDTLQFQESVHVEASTELSAVRGAEIVLFCVKTIDNAITARALAPLLAPGA
                     LVISMQNGVDNVEQIRAAAAIVALPAVVYVAASVPEPGRVKHVGRGDLVVGPENEKTK
                     RIAALFLRAQVPCRISANIEGELWTKLVWNCALNAVSALGRAKYGLIAASADAWKVVE
                     TAVYEVLAVARAAGIHPPGLEDPKVALAGALKIATQMAEALSSTGQDMNRGKRTEIDS
                     LNGYVSCRGVALGVPTPVNHALYALVKLAEGRP"
     gene            complement(12602..13768)
                     /locus_tag="VGR03_16275"
     CDS             complement(12602..13768)
                     /locus_tag="VGR03_16275"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV1995890.1"
                     /translation="MATTSTSPHTDWRQEWFEIEDATYLNLAGQAPMPKVSIRAVQAA
                     LEAKKYPHQKPDSTFFEVPNRIRASIAKLIGGKPEEVALTSGASAGVAAVAYALTWKP
                     GDEIVTAKGEFPLQYTAWKPMEEREGLKLKIVAPKERFITSEDLIAAITPRTRVVSVS
                     LVRYDDGSLLDAARVADACHKQGALLLLDASQCCGAIPMDVNQLGADFIVSAGYKWLL
                     SPFGTGFFWVKSENLGLVRPGPFYWMAVAGSDNFAALNFEDPKPAASAKRWDSPEWAS
                     YFNFNLAAMDASVDFVVRMGPELVAAHNRKLIELMFDRLPKDRFVPASPLDAARRGPY
                     GCFAARSPEKTAEHYQHLRKENVVVSLREGNIRVSPHLYNTERDIDRLISVVTP"
     gene            complement(13869..14555)
                     /locus_tag="VGR03_16280"
     CDS             complement(13869..14555)
                     /locus_tag="VGR03_16280"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1995891.1"
                     /translation="MRRHTLLIDADDTLWENNAFFEKLIEDFISIVEPYGYTRAYIRH
                     ILNETERKNIRQYGYGVRSFGRSLEETYIKLADQMAKRETLALIHGRVIDLERTPPKI
                     LDGVPETLAYLTERHRLILFTKGEPAEQAAKVERSGLQGFFEFIEILPEKDSATYGGL
                     VHKHKIVKTHGWMIGNSPRSDINPAMKIGLNAVYIPHQHTWTLEHEPVVAGSGKLVIL
                     PAFRELRGHF"
     gene            complement(14622..15761)
                     /locus_tag="VGR03_16285"
     CDS             complement(14622..15761)
                     /locus_tag="VGR03_16285"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1995892.1"
                     /translation="MKPKPRVLLLSATSGAGHVRAAQGLEKAFLARGDCAVEHIDTLQ
                     HVSRLFQRVYDKAYISMVRRAPQLMGVLYERTDKPWLHPRRRLALDRLNTGPMIHLLK
                     RIQPDLCVATHFLPAGIIAWLIAKRKLRAHNAIVVTDYDVHAMWLCRTVDRYYVAIDE
                     AAEYLARIGVPREKLRVTGIPIDPLFATPVNRSDARKQMGLDADATVILILAGGYGVG
                     PVEQLVKDLLALQRPWQIAAIAGKSDKTRKRLEELAKEAGNLSSGSPRLCPVGFTTEM
                     DKYMAAADLMVGKAGGLTTSEALAQALPMALIEPIPGQEERNADHLLEAGAAIRCNNL
                     PAAAWKIAALLDDFARFARMREAARSLGRPGAAAAIAEDAVRLLD"
     gene            complement(15793..16020)
                     /locus_tag="VGR03_16290"
     CDS             complement(15793..16020)
                     /locus_tag="VGR03_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995893.1"
                     /translation="MYHYDAKIALEELQEEALLPHPVKLRDMILRTKLGPQDAQLLNH
                     DFQDYLTRFGDLQKVARGILEKIAAGQRKTS"
     gene            complement(16110..16445)
                     /locus_tag="VGR03_16295"
     CDS             complement(16110..16445)
                     /locus_tag="VGR03_16295"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEV1995894.1"
                     /translation="MGDGAERREARRFTMSLPMRVLPREAKGRELDAHTRDVSYRGLY
                     FLADANFEVGSEIEFVITLPQQVTQSGDVNIRCAGEIVRIEPVEKGRVGIAAKIARYE
                     FMPAAANAA"
     gene            16612..>17085
                     /locus_tag="VGR03_16300"
     CDS             16612..>17085
                     /locus_tag="VGR03_16300"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEV1995895.1"
                     /translation="MDSRQHRRVRMRLPVRLRWTTPFGQKIELADTIDVSRSGLLVST
                     KESHTPGVVVWVTFPYDASLSDGQPEILSRVARCGEVLEVIRATNAREKVQSERASEQ
                     ERSAKLDQLARALGISDAPATFAVAFHFDEQPHSSSNGNAHRRELERRDSMRKALA"
BASE COUNT         3663 a         5181 c         4779 g         3462 t
ORIGIN      
        1 tctttgcagc tcacgctctt ttcaaaaaca ctcatcgcaa gccccccaat tcatggcgga
       61 gtttgcgcgt ggcgcgaaaa agcgaatttt taacggtttc ttcggtggtg ccgagggcgt
      121 caccaatcgc ccggagcttc aggccctgat agtgcttcat ctcaaaaacg atgcgttcgc
      181 gtggcgaaag acgctccatc gccgtagcta tgcgcgcctt gatttccttg ccgatgaagg
      241 tgcgttcggg gtccagcgtc ggacggtgct cacgttggcg ctcgaaaaaa tcggtgatcc
      301 cttcctcgtg gaggccggaa ttcagttcag gcgcctgatc ctcagggcgt gcttggcggc
      361 ggcgcaggtg gtccaggcaa acgttggtga cgatgcggta gagccaggtg tagaagctgc
      421 actcgaagcg gaaacggtgg agattgcggt agaccttgag gaatgcttcc tggtagacgt
      481 cccgggcgtc ctcggtgcgt ttcatcagat tcagcgccag gcgcagaacg tcccggtcga
      541 agcgacgcac cagttcctcg aaagctgcac ggctgcccgt ttgcgcttcc gcgaccaggg
      601 cccgctcgtc gaggtgggac aaagagatca ccgaggccaa ggcggcgtcc gtgctacccg
      661 agcgaccgtt gttatcggcc aaagccagag cccatctggg aagcggcata tttcgctttc
      721 ttttcgcgtg cgctggtgtt gccgcttgta aaacaacacc ttagttcaaa atccaccact
      781 cggctaaacg ctgcgcacta cgcgaccacc caatttggac gcactgcaac gcccttgggt
      841 gagcaccact tcccattata acaacggcga acctgtggat tttgtttcag gaattaacgt
      901 caaacgaggc acacaagagc aggtttgagt ccttggttga tctcctagag gccagaatcg
      961 agagctccca aagtcctcgc tgaatcgccc atgcgccagg caaccaacgg ccaggcgaac
     1021 agcaggacaa gcgcaagcag cagagcatag ccattcgcgc gataaaacga atccggctgc
     1081 gagagcagtt cggcggcgcc accgaataag gaagtcccgg cgacgatgag gaagcagccg
     1141 agaatattga aacgcataac gtagcgcacg ccgaaaacga gaacgctgag caggatgagc
     1201 tgcgccaaaa attccaccgc aaggtcgcgc ggactacccc agccaccgct cacgagtgaa
     1261 agcgagccaa cgagaagaag cacaatgcgg agagctggct gacgcacatg cgccgccaca
     1321 aaagaagcaa tcaacgcaac gaggccggtg agaaacaaac cgcgcacgac tgctcctcca
     1381 aggatcgaag cggaaggcag aagcgcgtcg aagtcctggc cgaacgaagc ttccagcgac
     1441 cggtgcacgg tcggccagtg cgtcgaagca gccacgagga ggcgctcgaa tccaagaaga
     1501 cctgccgctc ctcccaggcc catccagagt gcgtcgcgat aataattcgc gggcatctcc
     1561 gcccaagctg gcaggcggct aacctcgaat gcgcgcgtgg cgtaatacca ggccatgcca
     1621 aacagcagcg cgatgccgcc gaaattaaaa agagcaccga acagcgcgcc gatggcaatc
     1681 ccgccaaaca tcaacttgag gggaatggcg gtttcgtagg cgttcaagaa gctggggacg
     1741 cggttgccga gcaggaaaac caccagataa ccgcagagac cccacaccga ccagaaccca
     1801 atgcgcctcc aaggaatcga gcgcgctgtt tccgatttca gatttttcag aaacacaatg
     1861 agcgcagtga ggccgagacc caaaaagaac agaatgggga ctacgtagcc gacgatgatg
     1921 cggaagagtg tgcgttcctc ctgcttgcgg cgccattcct cgggaatctt gatgtacgtg
     1981 cgataacttg cgatttcgtc gcccagaagt agcacatcga tgcgggcatg tgcgtgatcg
     2041 gtcacactgg acgtcgtgta ttgtccggcg tcgagtggct cattctcttg ccaggttagc
     2101 gtgtgatcga tgcgatgcgg tttcttgtcg gaattggatt caacgagcgt ccaggccttc
     2161 aagtcgatct tcttttcttc agcaaggaac ttcttcgccc gcgccgcggc ttcttctttt
     2221 gtgagtgctg cgcccggcgc ctcctccgca agagtgtgcc ggactgattg gacggatccg
     2281 tcaggcgtga ggatgatcga aaactcctcc ggctggctgt cgcggaaata acgaacgtgc
     2341 cagagcgcgc ccggaacgcg ctctttgtag atcgcattga cccccgctgc gccaatccgc
     2401 tctcgtagat attcatttgt gaaagcgttg gtagcaggat cggggaaatc gacattgctg
     2461 cgaggatgag gagtatcgat gaagatggtg gcgtggtgaa aggaatttgg atcgaggcct
     2521 cgttgtctca tcacctggtc ggcgcgagcg cgagcagatc gcgcatccac ggacagtttc
     2581 agatagtcac cgacagactg cggcttcaga cgccacagta gcgcgcctcc agccagcaaa
     2641 cagacagaga ggaaagcgat catgcccggt gcgagtgcat cgtagcgtcg tgacgtgact
     2701 tcgacagttt ctgagacggg ttcgctggtg agatcgagtt tgggcgagcc agcagagcgg
     2761 ttgagaagat cttcgtcact ttcgaatccg ccgcgggtca agtaggagat gcaggcaaag
     2821 actaaaggtg cgagggcggc cgcggccacg accatgccgg atattttaaa gtagagacta
     2881 ttggaacgga tcaaaagcaa cccaaccagc gacgcatcca cggtgtagtg ccagatgagc
     2941 gtcgcgagga tgccccagcg caacatcacg atgcctgcaa caattccgat gatcccaatt
     3001 tcgattccgc ggatgtaggc cggttcctgc gggtaattgc tgtgcagaaa gctccaaaga
     3061 aaagccggca cgatcacggc cacccagcgc gaacgcgtaa atcgcatgaa aaacggaatc
     3121 gcgaacaacc ggaacgtgaa ctcttcgttc atggaagcga gcagcccgat cgcgatgccg
     3181 gaaatccacg ggaacgccgt attcacgctg tccgaatagt tcaactcctg cggcgcccac
     3241 gcccccagac gagtggcgac gatgtagaag gcgacaataa aaccaatgtg cgcggcagcg
     3301 aggcacaacc ccacgacgga ggaagagaaa aactccttcg aacgcagccc gcgccgcgtg
     3361 aagatttgtc ctaatcttag ctggcccggc tgggatttgc ggtagagcgg ctccgccgca
     3421 ggcaggacca gcgtaatggt gagcgcagag gccgccgcaa gcagcaaggc acgcccgatt
     3481 tgcagaaaga taaaggtgga gtaggagtcc tttgtatcat atcccgctcc ccagagcggc
     3541 cagtcgttca gggactgcag gaataacatc acggtcgcga gcgcaccaag cttgagcgcc
     3601 gcgctccagg ctgtctcccc gctcttcgtc aactgaattg ccagccaaac agcggttccc
     3661 agaagcaaca ggtagggaat agtgaagatt aaggcgagcg tgttgtttcc ggagcgcagt
     3721 ctttgataac cgcgttccca cgcttcgggg acgcgcaaga actctccgct gccgccgatg
     3781 cggtcgcctt gtaaagtgac ttgcagccgg tacggcgcgt gtttagcgcg gaagccgtgt
     3841 ttttcccacg taaaagccca atcaagacgg tttggccgct tattcgaatt ggcttcttcc
     3901 gggagaagat cccagccgct cagatcgagt ccgagtttcg cgttgagata agtctgcgcg
     3961 gcggattggg cggtggcgcg atcgagcgag gctccggcgc gggattcctc gattttgtga
     4021 tcgtagctga tgatctgtcc tgccgggctc acgcgaacgc taaattcctc ctcctgctgg
     4081 ggtttgaaaa agcgtacgtc ccagaaccag atattcagct ccgaagacat gagtcgattt
     4141 gcttcttgca ggccgagttg gcgttcgagg tagactttgg cgttgtcgtc gacattgaaa
     4201 acaattgtcg attcgtagcc gctgacgttc tccccaaggc cggtgacgaa tttctgcgcg
     4261 cgcgacagcg cttcttcgcg ggagacttga aaattaacgg aggcttcggg aaaagcctgg
     4321 aaaaaatatt tgtaggcgaa cagcgcgccg aggattcccg caaacaccca gaggagcagc
     4381 gcgcgcttgt cggaacttgt cagacggtct cccgtcacat gaacctcccc gttcgggaca
     4441 cggaatcctc actctatcga tacggatagc tgggacgcaa gtatcgccgc tgcaatatgt
     4501 tcgtagcggg aatgatataa agttgatgtc gtcggccgtt ttacggccgc gcttgcaatt
     4561 ggccgttttg tggccccacg tactcatggc gatgaacctc agcaccgaag ttcgcatgat
     4621 caaaggcgtt ggcccgcagc gcgccgaatt gctcgcgcag cgtgggatct acacgctcga
     4681 ggatctactc ggctacttgc cgtttcgcta tgaagatcgc atccattttt ccaaggtgaa
     4741 agacatccag ccgaacggta cgtataccat tcgcgcgcgc gtgctgagcg gccaggcggt
     4801 gcgcagcatg cgcggcggcc gcgacgccat ctatcatctg ctcgtccagg acgacgccgg
     4861 cggctcgctg ccctgcaagt tttttcacgg tggctacctc gaagggcgct tgaaacccgg
     4921 ccaactcctc atccttcatg gcaaagcgga gatcgataga ctgcggcccg cacgcctgga
     4981 aatgatcaat ccgcagatcg aagtgctcag caacgaggag atggattcca ccgaagtggg
     5041 gagaatcgtg ccgatctacg aagcgattgg cacatttggc tcccgtcaga ttcgccgcgc
     5101 gatgtacgcc gccgtgcagc tcatcgattc gagaatgccg gatgttttgc cggaaggatt
     5161 gcgcgcgcgg ctcggctatc cctcgcgcgg cgaagctctg attcacaccc actttcctga
     5221 gcccggcgaa tccctcgatg ccttgaatac gttccgctcc acggcgcaac agcggctcat
     5281 cttcgaagag ttttttctct atcagctcag cctcgctttg gaccgcaagg ccgatcgcaa
     5341 ggaaaacgcc atcgcgtttt gcgtgcgcga agacgccgtg cgcgaagcgc tgaagcgtat
     5401 tctcccgttc aagcccacgg ccgcacagaa acgcgttctc gccgaaatcg ccgcggacct
     5461 ggaaaagccc gcccccatga atcgtctcct ccagggcgac gtcggcagcg gcaagaccat
     5521 catcgcgctg caagcggcgg tgatcgccat tgaaaacggc acgcaagcgg cgctcatggc
     5581 ccccacggaa attctcgccg tgcagcattt tctctccgcg cggcgcctcc tcggcaaagc
     5641 aggctatcgc gtcgagctgc tcatcagcgg tctgaaagct tcggagaaag ccgcggcact
     5701 cgatcgcatt cgttccggcg aagcccaact cgtcatcggc acccacgcgc tgatcgaaga
     5761 caacgtcgaa ttcgcccgcc tcggttttgt cgccattgac gagcagcatc gcttcggcgt
     5821 cttgcaacgc aagcgtctga tggacaaagc cgcggcgcat ggccacgccc cgcatgttct
     5881 cgtgctgacg gcaacgccca tcccgcgcac gctgtcgctc actctctatg gcgacctcga
     5941 tgtttccatt ctcgatgagc tgccgccggg gcgcacgccc atcggcacgc gcatgaccac
     6001 ggagcagcat ctccccggcg tttgggaatc tctccgccgc gaagtcgctg ccggacacca
     6061 gggctacgtc gtttatccgg tcatcgagga atcgaaactc gagctaaaag cggccgtgga
     6121 agaataccag cgcttgtcgc gggaagtttt tccgaagctt aagctcggtc tgctgcatgg
     6181 ccgcctctcc agcgaagaaa aagaagaagt catgcagcgc ttccgcaaga atgaagcgca
     6241 gatcctcgtg gccacgaccg tcgtggaagt cggcgtcgac gtcccgaacg ccaccgtcat
     6301 ggtcatcgag cacgccgagc gcttcggtct ctcccagctc caccagcttc gcggacgcat
     6361 cggccgcggc gcgcagaaat cccattgcat cctcgtcgcc ccttttcgca tgaccgacga
     6421 agcgcgcgcc cgcctcgaaa ccatggtccg cacctccaac ggtttcgaaa tcgctgaaac
     6481 cgatctcctc ctccgcggcc ccggcgaatt tttcggcacc cgccagtctg gcgacctcgg
     6541 cttccacatc gccaaccctt tgcgcgatcg cgaactcctc gaactagccc gccgcgaagc
     6601 tttcgccctc gccgccgatt ccgcccagca cgaaaccatg cagcgcctcc tccgccagct
     6661 cccccccgaa tggcagcgcc gctaccacct cgcccgcatc ggctgatgtg tttgcgcgat
     6721 ctcacatgtc tactttgtgt gttttatgcg tcgtatttcc gggtcaatct cttgggttat
     6781 actcaggtag ggtaaggagt ggaactaggt tagttttgcg catggtcgcg gaccgataca
     6841 ctgcttacag aagtcgaaga gactgagatg aatatcgtat cgactgggtt aagcgcaaag
     6901 acggttgcca actatttcct tgatttggcc tcgagagacg gcgtcccgat tgatccacta
     6961 aagctccaga agctcatcta tcttgcgcac ggatggtcgc tcgtgcttct taaaaagccg
     7021 cttatcaaag agccatttga agcatggaat tacggccctg tggtcccatc actttataag
     7081 gcgttccaga aatttggggc ttcgccaatt actgaacatg cgccgaaaga accattcgaa
     7141 agccattacg agttggatgc tcagacaaaa tcccttttgg actcggtttg ggaaacctat
     7201 aaaaagttga attcaattca actctcaatg tttactcatg aaccgggata cgcatgggac
     7261 ttggcacggc ggggaggacg catgtctcca tggggcagcc cggcgatccc aaatgagctg
     7321 attgcaaatg agtttatccg ccgccaacag caggcagaat gacacgatat gaccgatcag
     7381 cctgccgcca aaaagcccga aaaatccctc aaaatacccg acccaagaat cgaagagtta
     7441 aaggtttctg ccgcagacag catctctctt gctgacgccg ctcaggaata tcgtggcttt
     7501 ctatcgcgga acaaatcagc gcttacgcgg acttggtggg attggctgct aacatatgtt
     7561 ttgaaactga ttctgtttgg cttcgttctt gcgataaata tctggtggac tcgcaatgtc
     7621 ctaaaaatgg tttggctatc aggttcgcaa ggtagccact tccaccttga taacaacgtg
     7681 ctgataactc tggtttctac gtcaatcgcc agttttttgg gtttggtggc aattgtcacc
     7741 aaaaaccttt tccccgattc ctccaaataa aactggctgt cctggcgatg gcgggctaac
     7801 ccttgagtag tcgagttatc aggcaaatgc acgtcggatt tcttggcagc gttccaagca
     7861 acctgagacg tgactgtcct atcgggttga gaggttaacc tcgggaaaat gaatgttcag
     7921 cttatagcaa ataaaaagaa ccagcatcgc gcccagtgtg gaaaaaatga ggccggcggg
     7981 atgatatcgt tcgtttgtcg ggcgcgaaag catgtgggta aggccgccgc cgatgatcga
     8041 tccgatgatg ccgagcacga tcgtccagaa gatcgttata tgcacgtgca tcaccgattt
     8101 cgcgatcacg cccgagatta gcccaatcag aacataccag attaagtgaa acatactttt
     8161 tcctccctct ttctcgcgca ccgtacgctc gccctgccct tgacacgacg aatatgccac
     8221 aagagcaagc ctcgccgaga tcagttggct tcccgcttga ttgagggact ggcgaacaaa
     8281 acagtctttt tgctgtccgc caactgtagg ctttgccaat gcctaaagct gtccactttt
     8341 agacagccgc atcacttcaa gacggcttaa tgattcgaga ttctttgttc cttgactaaa
     8401 aaccaaaaac tgacaactaa taactgcact catgcgcgtc atcgccggaa cctaccgcag
     8461 ccgcatcctg aagagcctca agggcctcgc gcggagccaa accggaatga aggaaacgag
     8521 tttctcccgc aggcgcaaat cctagggagt ggccgtctcc acaattagcg accgattggt
     8581 gacaatggcg gaagttggat tgcccatgcc ccagaggatg tcgaccgtgt gagaacccgc
     8641 agagatgttt tgcgcaaccc agtgaaacga tcgcgtgtcg caacagaact gcgggtagag
     8701 gaattcgaca gtgttggtat ccggctggca agcttgaccg tcgatgtgac actcgataaa
     8761 cactatgggg atgatctgac tgccggaagg tgcaactgag ccgcgggcac cgaacgacac
     8821 cgtgagaaag cccgaagact gcgactggaa ggtcaaagat gtggatggca tcagttcaag
     8881 tgcgccgctg ggattcgaat aggttgctgg gctggaagtc gccagggtaa agacatgagc
     8941 cagttgcccg gcttgagtct gctgcggcgc ttgcggtgga tcgttttgtt gcgccaagtt
     9001 cgccgcaaca tttccgcagc cagaactgaa agccagtaca agtgggggaa gaaacgctcg
     9061 accgaagacg ctttttctca tggtgggcct ctcaaataaa cacgggccgt cacccggtgg
     9121 cggtctaagc tgcgagccta cgccggagtt atcttcttgt caaagcagag gtcaatacaa
     9181 ctacccggat tctttgttcc ttgactaaaa accaaaaact gacaactaat aactgcactc
     9241 atgcgcgtca tcgccggaac ctaccgtagc cgcatcctga agagcctcaa gggcctcgcc
     9301 ctgcgcccca ccagcgaccg cctgcgcgaa actctcttca acgttctggc ccccaatata
     9361 gccggctctc gcttcgtcga tctttttgcg ggaaccggcg ccatcggcat cgaagccctg
     9421 agccgcggcg ccgccgaagt cgtgttcatc gaaaatcacg cgcccgccgc cacgttggtt
     9481 cgccgcaatc tcgaatcgtt gggcatcagt accggcgtga ccgtgcttgc cgtagacgct
     9541 cttcgcgggc tcgcaatgtt ggcttcccga aagaatcagg ccgaacccgg cttcgactac
     9601 gtttttctcg atccgcccta cgctgctgcc gaagactact cccgcgtgtt agaatttcta
     9661 ggttccgcgg atattctcgc gcccggcggc atggtcgtcg ccgagcatcg ccgcaatttt
     9721 gatttacccg aagaacccgg cgcactacgg aggttccgcg tgctcagaca gggcgatgca
     9781 gcctttagtt tttactgccg ccgcggcgag gctggcggcg aaaataactc tgccgagtaa
     9841 acccccgcag gcggagtctc atctattctg aagtgctcgc gacctccgtt gtcgccaagc
     9901 agagggccgc gcgcggcaag gtacaatcga gaggaacatg tctctgaacc aggccagcaa
     9961 cgcgttcgat cttctggggc agtcgggaga acccgccgcg cccgtggatc tccagactgg
    10021 ggaagggccg aagctggcag ttggctgggg gagctttcat caggggatcg gaagcagcat
    10081 cgcggctctt ttcagccgaa cggtcgttcc aaaaaattcc gcggttggcg atttcttcaa
    10141 ggattgctgg attgagcgcc gcattcctcg ccgagccctg attgccgccg cattgtggca
    10201 cttcgttttt gtcctcatgc cattcccgca gctccccgcc gcggctcgcc actcttccgc
    10261 attggataac accgaactca cctggtccgg cccgatcaat gattttccat tgatggagat
    10321 caagagtccg aaggccaaac caagtccgcg cggcgaaccg gcgaaacctc tccctcgcga
    10381 gggcgccgac gccttccatc cccggcagcg catctttacc gatcccgtgc gcccgacgca
    10441 tccgcgccag acgttgatca attctgccgc tgcactcgag ccgccgaaga ttcttccgaa
    10501 ccttcccaat atcgtgcaat tccagcagat ggccggcccg gcgcgcccgc gtctgcagat
    10561 ctgtcaagaa acactctcca agctgcgccc gcgtgagctt cgcaaggtta agttgacctc
    10621 cgcgccggct cccgaccttc caacgctgga ccaaaaactc tcggatattg cactctcaat
    10681 gttgccaaac gctcccgcgc gtcccaagct ggaattgaac gccggcgccg ccccgcgcgt
    10741 cgcgccacgt gcacaagctg gtgaagtaga acccgcaccg gaagtcggct ccgttcaaac
    10801 tggcctggcg aatggaaatg ccgcaacgtt catcgccctc tcagcgacac ccggtcctcc
    10861 cgcgcctgtc gtgcagccgc cgcaaggaaa tctagccgcg cgtgtctcca tttcaccgga
    10921 aggcaggcaa cctggcgtac ccggaggctt gccgaacgga atgcccggtg ccaacggcgg
    10981 cgcgggtggc ggaccggaga gcagcggggg aaccgccacg ggcaacggcg gcggaaaaaa
    11041 tggtaaggac gtgagcatca ccggaggaca cccgccggca aacaacggga tttccggtct
    11101 cggcggatca gccaaaatca gcgcacccat tccgcacgcg ctgatcctca agcctgatcc
    11161 gcacaccaag atcgatgatg cacccgagag aaccggccca ccgaatttcg aggcgctccc
    11221 ggccggtgcg ttgcccgagc agatcttcgc ctcgaaaaaa atctacaaga tgctcgtcaa
    11281 catgcccaac ctgaacagcg ccacgggaag ctggattctg aatttctccg agctacgcgt
    11341 gaattccgaa ggcccccggc tggcctcgtc ggacgtttcc gctcctgggc ctttaaggaa
    11401 aatcgacccg aagtatccgc ccacattgat caaagagcgt gtggaaggcg aagtcgtgct
    11461 ctacgctgtg attcgccgcg acggctcggt tgacagtatt cagttggtgc gtggcatcga
    11521 cgagcagctc gatgccaacg ccatggaagc actgagccag tggaagttcc ggcccgcgac
    11581 gaaacaagga acgccggtcg agctcgaggc catcgtccac attcctttca atcccccggc
    11641 cgagcgttga gaattcacat tttttttggt ttggactacg gacgcccttc ggccaacttc
    11701 acaagcgcgt agagcgcgtg attgacgggc gtgggcacgc cgagcgccac accgcggcaa
    11761 gaaacatatc cattcaaaga atcgatttcc gtgcgcttgc cgcgattcat gtcttgtccg
    11821 gtcgaggaaa gcgcctccgc catctgcgtg gcaattttaa gggcgcccgc gagcgccacc
    11881 ttgggatctt ctaggccggg cggatggatt ccggccgcac gcgcgacggc caggacttca
    11941 tagactgccg tctcgacaac tttccacgca tcggcactcg cggcgattag gccgtacttc
    12001 gcgcggccca gcgcggagac ggcgttcagc gcgcaattcc acaccagctt ggtccagagc
    12061 tcgccttcga tatttgccga aatgcggcaa ggcacttggg cgcgcaaaaa aagtgccgct
    12121 atgcgcttcg tcttctcgtt ttccggaccg acaaccagat cgccgcggcc gacatgtttc
    12181 actcggccgg gctcaggtac ggaagccgcg acgtaaacga cggcgggaag cgcgacgatc
    12241 gctgccgcag cgcgaatctg ttccacgttg tccaccccat tttgcatgct gataactaga
    12301 gcgccgggag caagcagcgg agccagcgcg cgcgccgtta ttgcgttgtc tatcgtcttc
    12361 acgcaaaaca agacaatttc cgcgccgcgc accgcgctga gttcggtgga cgcctcgacg
    12421 tgcacactct cttgaaactg caatgtgtcg aggaagagtc cgcacttctt tacggcttcg
    12481 acaaaggccg gacgcccgac catcgccacc ggcgcaccgg cgcgcgccag cagtccgccg
    12541 aagtatccgc ccacggcgcc cgcaccaacg acggcaatct ttggccagct gtactgctcg
    12601 gtcacggagt gaccacgctg atgagccggt cgatgtcgcg ctcggtgtta tacaagtgcg
    12661 gggagacgcg aatgttgcct tcgcgcaggc tgacgacaac attttctttt cgcagatgtt
    12721 ggtaatgttc cgctgttttc tccgggcttc gtgccgcaaa acatccgtac gggccgcgcc
    12781 gcgctgcatc cagaggactc gccggcacaa accgatcctt cgggaggcgg tcgaacatca
    12841 gttcgatcaa tttccggttg tgcgccgcga ccaactccgg tcccatgcgc accacaaaat
    12901 ccacggaggc atccatggcc gccaggttga aattgaaata gctggcccat tccggcgaat
    12961 cccaccgctt cgcgcttgcc gcgggcttcg ggtcttcgaa gttcaacgct gcgaaattgt
    13021 ccgagcccgc caccgccatc caatagaacg gtcccggccg cacgagccca agattttcgc
    13081 tctttaccca aaagaatccc gtgccaaagg ggctcagcag ccacttgtac ccggcggaca
    13141 cgatgaagtc ggcgcccagt tgattgacat ccatagggat tgcgccgcag cactggctgg
    13201 cgtccagcaa cagcagcgcc ccctgtttgt gacacgcatc agcaacgcgc gccgcatcca
    13261 acagcgagcc gtcgtcgtac ctcaccaggc tcacggacac aactcgcgtc cgtggagtga
    13321 tcgcagcaat caaatcttcc gaagtaataa atctctcctt cggcgcaacg attttcaact
    13381 tcagcccctc gcgttcttcc atcggtttcc acgccgtgta ttgcagagga aattcaccct
    13441 tcgccgtgac gatctcgtcg cctggcttcc acgtcagtgc gtacgcgacc gccgccaccc
    13501 cggcgctcgc tccgcttgtc agcgcaacct cttcgggctt tcccccgatc agcttcgcaa
    13561 tagatgcacg gatgcggtta ggcacttcga aaaaagtgga gtccggcttc tggtgcggat
    13621 atttttttgc ctcgagcgca gcttgcacgg cgcgaatgga aaccttcggc attggcgctt
    13681 gacccgccag attcaagtac gtcgcgtctt caatctcgaa ccactcctgt cgccagtcgg
    13741 tatgcgggga agttgaagtc gtagccacgg gtttctcctt cgtgcgggag tcacaacgga
    13801 ttagattcga gcatacagcg tcgggtacaa cgtccaattt gggccagtcg gtccagacta
    13861 gacacagtct aaaaatgacc gcgaagctcg cggaaggcag gcaggatcac tagttttccg
    13921 gagcccgcca ccaccggttc gtgttccagg gtccaggtgt gctggtgggg gatatagacg
    13981 gcgttgaggc cgatcttcat cgccggattg atgtcgctgc gcgggctgtt gccgatcatc
    14041 cagccgtggg tcttcacgat tttgtgtttg tgaactagtc cgccatacgt cgcggaatct
    14101 ttctcaggaa ggatttcgat gaattcgaaa aatccctgca ggcccgatcg ctccaccttg
    14161 gcggcctgtt ccgcgggctc gcccttcgta aataaaatca gccggtgccg ctcggtgaga
    14221 tacgccaggg tttccggcac tccgtccaga attttcggcg gcgtccgctc caggtcgata
    14281 acccggccat gaatcagcgc cagcgtctcg cgtttcgcca tctggtccgc cagcttgata
    14341 taggtctctt cgagcgagcg cccgaagctg cgcactccgt agccgtattg ccggatattc
    14401 ttgcgttccg tttcattgag gatatggcga atgtaggcgc gggtatagcc atacggctcg
    14461 acgatgctga tgaaatcctc gatcagcttc tcgaagaatg cgttgttttc ccagagcgtg
    14521 tcgtccgcgt caatcagcag tgtatggcgt cgcatcgaac ttggcgatcg gcggcgttcg
    14581 cgccgcgaac cgctttcccc cagccgcttt ccccagtgtc ctcagtctaa cagccgcacg
    14641 gcatcttcgg cgatggccgc ggcggctcct ggtcggccaa ggctcctcgc agcctcccgc
    14701 attctcgcga atcttgcgaa atcatccagc agcgccgcaa ttttccacgc cgccgcaggc
    14761 aaattgttgc agcgaatcgc tgctcctgct tccagcaaat gatcggcatt gcgttcttcc
    14821 tggccgggga tcggctcgat tagcgccatg ggcagcgcct gcgccagggc ttccgacgtc
    14881 gtcagcccgc cggccttgcc caccatcaag tctgccgccg ccatgtactt gtccatctcc
    14941 gttgtgaatc cgacggggca aagcctcggc gagcccgaag agaggttacc ggcttccttc
    15001 gccaattcct ccaaacgctt tcgagtcttg tcagattttc ccgcaatcgc cgcgatttgc
    15061 cacggccgct gcagcgccag caggtctttg acgagttgct cgaccggacc cacgccgtaa
    15121 ccgcctgcca agatgagaat taccgtcgca tccgcgtcga gccccatttg cttccgcgca
    15181 tcggatcgat tcaccggcgt cgcgaagaga gggtcaatcg gaataccggt cacgcgcaat
    15241 ttttctcgcg gcacgccgat gcgcgctaga tattccgcgg cttcatcgat agccacgtag
    15301 tagcggtcca ccgtgcggca aagccacatc gcgtgcacgt cgtagtcggt gacgacgatg
    15361 gcattgtgcg cgcgcaactt ccgcttcgcg atcagccacg cgatgatccc cgccggcaga
    15421 aaatgagtcg ccacgcaaag atccggctgt atgcgtttca acaggtggat catcggaccg
    15481 gtgttcagcc ggtccagcgc caggcggcgc cgcgggtgca gccacggctt gtccgtgcgc
    15541 tcgtaaagca cgcccatcag ctgcggtgcg cgccgcacca tcgagatata agctttgtcg
    15601 taaacgcgct ggaaaagtcg gctgacatgc tgcagggtgt cgatatgctc cacagcgcaa
    15661 tcgccccgcg ccaggaaagc cttttcgagg ccttgcgcgg cgcgaacgtg tcctgcgccg
    15721 gacgtcgcgg atagcaacaa cacgcgcggc tttggtttca tttcccggga attcctgcga
    15781 atgcaagtgg cattaggaag tcttgcgctg gcccgcggcg attttttcca gaattcctcg
    15841 cgcgactttt tgcaaatccc cgaagcgcgt caggtagtcc tgaaaatcgt gattaaggag
    15901 ctgcgcgtct tgcgggccca gcttcgtcct cagaatcatg tcacgcagtt ttacgggatg
    15961 cggaagcagt gcctcttcct gcaattcctc gagcgcaatc ttggcgtcgt agtgatacat
    16021 agcttccccc agcagaaaaa tccttccacc gtattgtact cagttgttgc cgcgtaaggc
    16081 gagttacccg aaaagtgggg ggaagagtcc tacgcggcgt ttgccgctgc aggcatgaat
    16141 tcatagcgcg cgattttcgc ggcaatgccc accctgcctt tctcgacggg ttcgatgcgc
    16201 acgatttctc cagcgcagcg gatatttaca tcacccgact gcgtgacttg ttggggaagt
    16261 gtgatgacga actcaatctc gctgccaact tcgaaattcg catcggcgag aaagtacagc
    16321 ccgcgataac tgacatcccg cgtgtgcgca tccagttcgc gtcctttggc ttcccgcggc
    16381 aaaacccgca tcggaaggct catcgtgaat cgccgcgctt cgcggcgttc tgctccatca
    16441 cccatcaata ccccctgggg ctgtaggact gggcgcaaag ctaacatcgg ccccggcaaa
    16501 ctgcaacgcg aaaatcaggg aattttgtcc tagtacgagg tcttggcggt ttcaatacgt
    16561 gagccatata ctcaggctta taccagccct tagggcagta ctgttacgcc tatggacagc
    16621 cgccagcacc gccgcgtgcg gatgcgcctt ccggtgcgtt tgcgctggac tacgcccttt
    16681 ggccaaaaaa tcgaactagc agataccatc gacgtctcgc gcagcggtct gctcgtttct
    16741 accaaggagt cccatactcc gggcgtagtg gtgtgggtca cgtttcccta tgacgcctcg
    16801 ctctccgatg gtcagccgga gattctctct cgcgtcgccc gctgcggcga agtgcttgag
    16861 gtcattcgcg ccaccaatgc acgcgagaaa gttcagtcgg aacgcgcgtc ggagcaggaa
    16921 cgctctgcga aattggatca actcgctcgc gccctgggca tttccgatgc cccggcgacc
    16981 ttcgccgtcg cttttcattt cgatgagcag ccgcactctt catccaacgg aaatgcccat
    17041 cgccgcgaac tcgaacggcg cgacagtatg cgaaaggcgc tcgcg
//
LOCUS       DASYVO010000475         3866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_68205, whole genome shotgun sequence.
ACCESSION   DASYVO010000475 DASYVO010000000
VERSION     DASYVO010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68205"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VGR03_16305"
     CDS             <1..834
                     /locus_tag="VGR03_16305"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV1995896.1"
                     /translation="AGVAKLTRDAIAKFHDTWYRPNNTTLVIVGDTTLAEIKPQLEKT
                     FASWKQGNTPKKSISNVAAPKQAEVFLIDKPGAAQSFILAGTIAPPRNSPQEIALGIW
                     NDILGGTFGGRVNMNLREDKHWSYGARTAFQFARGDSAWVAQAPVQTDKTKESLVELN
                     KELRDVFGARPISQDELNKDKDNRTLRLAGSRETMAEVGDAIENVVQYRLPDDYYSTY
                     ASKVRALTTNDLTTAGKSFLNPDRLVWVVVGDLAKVEAGIRELNLGTVHVVDADGNSI
                     R"
     gene            complement(934..2241)
                     /locus_tag="VGR03_16310"
     CDS             complement(934..2241)
                     /locus_tag="VGR03_16310"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEV1995897.1"
                     /translation="MGEAPLTNGRSNTTIFLAHVGAFLFVVLAAYWLRRITLQPDAIL
                     QGYLQVVSGLLAFVFAAVTLVRFQGTQDRISLILGAGFLLSGAVLTATSVLFFQFFPD
                     APRLLWAPIAWWLGRMVLALLLVVALLVERFLPRSRHPRREIAGALLTVVALTYVLAA
                     TLRGLPPEVSRHPGAFFPNPEQLLPGAIFLVSLIWYRRRLSVEDSAFDRTIYAATWLN
                     VAAQLSAAQSARLLDAPFVFAQALMVLGYTVALGGALLDNARLFEQVRHLAVSDPLTG
                     LANYRRLLDVLENETERSDRTGRPFSVLLLDLDDLKKINDSYGHLVGSRAICRLADIL
                     RIHCRAVDTAARYGGDEFALVLPESQEDDARRVANRIREVLANDGEQPPLSASIGISI
                     YRGDGERIEKLLSEADQNLYVEKAKRGKRAAAAPNPRRRSPNS"
     gene            2616..>3866
                     /locus_tag="VGR03_16315"
     CDS             2616..>3866
                     /locus_tag="VGR03_16315"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV1995898.1"
                     /translation="MNGESTANSGPRLKRSLTLWNLIVIGIVIIQPIAPMGLYGIVNN
                     AAGGHVVTTILIAMVAMLFTAISYGMLARVYPSAGSAYTYVGQEIHPGLGYLTGWAMV
                     MDYILNPLICTILCSKLTQNILPGVPYWALAIFYATAFTVLNLRGVKTSARINDVLAA
                     GMTIVVIVFFGFVIRFLLGLHEYGAGFFSRPFYNPATFNLNSIFHGTSIAVLTYIGFD
                     AISTFSEEVENPRRNILLATVLVCVVTGVLSSLEVYAAQLVWGSRPFPAEQVESAFPL
                     VAKQVGGFFLFQLLNFTILIANIGSGMGAQLAAARLLYGMGRNDALPKSFFGKIEAKR
                     QIPANNVILIGAIALVGSFFLTYERGAEMLNFGAFIAFMGVNAAAFVRYFLRAERKKI
                     WPVLWPLLGFGICGYIFLQLSMPAK"
BASE COUNT          865 a          989 c         1258 g          754 t
ORIGIN      
        1 gcgggcgtcg ccaaattgac gcgcgacgcg atcgccaaat tccacgacac gtggtaccgg
       61 ccgaataaca ctacgctggt gatcgtgggt gacaccacgc tggcggaaat taaaccacaa
      121 ctcgaaaaga cctttgcgtc ctggaagcaa gggaatacgc caaagaagag catttcgaac
      181 gtagctgcgc ctaagcaggc cgaagtcttt ttgatcgata aaccgggcgc ggcgcagtct
      241 tttattttag cgggaacgat tgcaccgccg cgcaacagcc cacaggaaat tgcgctaggg
      301 atctggaacg acatcctggg cggaacgttt ggtgggcgcg taaacatgaa cctgcgcgaa
      361 gacaagcact ggtcttacgg cgctcgcacg gcgtttcagt ttgcgcgcgg cgacagcgcc
      421 tgggtggcgc aagcgccggt gcagacggat aagacgaaag aatctctcgt tgagctcaat
      481 aaagagttgc gcgacgtgtt tggcgcgcgt cccatctcgc aggatgaact aaacaaggac
      541 aaggacaatc gaactcttcg cctcgcgggt tcgcgcgaaa cgatggcgga ggtcggggat
      601 gcaattgaga atgtcgtgca atatcgtttg cccgacgact actactcgac gtatgcatcc
      661 aaggtgcggg cgctgacgac aaacgatctc acgaccgcgg ggaaatcctt cctcaacccg
      721 gatcgattgg tgtgggtggt ggtcggggac cttgcaaagg tggaggccgg aattcgcgag
      781 ctgaacctcg gcacggtgca cgtcgtggat gccgacggca attccattag atagaagtga
      841 ctaactgtga tgggagacaa ggcgaagacc agcgagcagc ttgcgcctta cgctgcaggt
      901 gattcgaaca aataggcaga cagctcgagc agtttaggaa ttcgggctac gacggcgggg
      961 attgggcgcg gcagcggcgc gctttccccg ctttgccttt tctacgtaaa gattctggtc
     1021 cgcttccgag agcagttttt cgatacgctc gccatcgccg cggtagatgg atatgccgat
     1081 actcgcagag aggggcggtt gttcgccgtc gttggcgaga acttcgcgga tacgattcgc
     1141 gacacgacgt gcatcgtctt cctgcgattc cgggagaacg agggcgaatt cgtcgccgcc
     1201 atatcgcgcg gcggtatcga cggcgcggca gtgaatgcgg agaatatcgg cgaggcgaca
     1261 gatggcgcgg ctgccgacga gatggccgta gctatcgttg atttttttca ggtcatcgag
     1321 atcgagaagc agcacggaga agggccggcc ggtgcggtcg ctacgctccg tctcgttttc
     1381 aaggacatcg agcaggcggc ggtagttggc aaggccggtg agcggatcgc tgacggcaag
     1441 atggcgaact tgttcgaaga gccgcgcgtt atcgagaagc gcgcccccaa gcgcgacggt
     1501 gtagcccaac accatgagcg cctgggcgaa aacgaaggga gcgtccagca agcgcgcgga
     1561 ttgggcggcc gagagctgag cggcgacatt cagccaggtc gcggcataaa tggtgcggtc
     1621 gaaagcagaa tcttcaacgg acaagcggcg gcggtaccag atgaggctca cgaggaaaat
     1681 tgcgccgggc agcaactgct ccggattggg gaagaaggcg ccgggatgcc gggaaacctc
     1741 cggcggcaag ccgcgaagcg tggccgcgag tacgtaggtc agcgcgacca cggtcagcag
     1801 agcgccggcg atttcgcggc ggggatgacg cgaacgcggg aggaaacgct cgacgagaag
     1861 cgccacgacg agcagaagcg ccaagaccat gcggccaagc caccaagcaa tgggcgccca
     1921 aaggaggcgc ggggcgtccg gaaagaattg aaaaaagaga acgctggtcg ccgtcaggac
     1981 agcgccggaa agaagaaatc cagcgccgag aatcagtgaa atgcggtcct gagttccctg
     2041 gaaacggacg agggttacag cagcgaaaac aaaggcgagc aggccactga cgacctgcag
     2101 atagccctga agaatggcat cgggctgaag agtgatgcgg cggagccagt acgcagcgag
     2161 gaccacaaat agaaaggcac cgacatgcgc caggaagatg gtggtgttac tgcggccgtt
     2221 cgtgagagga gcctcgccca tggggtatgc cgaactttcc tctattttcc gggccgggcg
     2281 cctacctaat tagactacgg aggaagcatt tgcgttgccg gagattcgag gagcgcgtga
     2341 ggcaaaagat accgtcctgt ggaattcgcg acctgtacga aagtgcagga caaaatgtga
     2401 caatggagat ccaacgggat gagtgagagt gcggaggaag cttttgcagt tgcgcggagg
     2461 agcaagattg cgcacactgt cagtattgaa acgattgaat acagaggaat acaagcgtgg
     2521 tgcgcggggc agcgggtttg caagaattcg cccgacccac atgctacggt gacgcagggt
     2581 tcggatcgtt gttattcagg gaaaaggaga attgcttgaa cggcgaaagc actgcgaatt
     2641 ccgggccacg cctgaagcgt tcactgacgt tgtggaactt gatcgtcatc ggcatcgtga
     2701 tcatccaacc gatcgcgccg atggggctgt acggcatagt gaataacgcg gcgggcgggc
     2761 acgtggtgac caccattctg atcgcgatgg tggcgatgct gttcacggca atcagttacg
     2821 ggatgctggc gcgcgtgtat ccgagcgcgg ggtccgcgta cacctacgtc ggtcaggaaa
     2881 ttcacccggg gcttggatac ctgacgggct gggcaatggt gatggactac atccttaacc
     2941 cgctgatctg cacgatcctg tgcagcaaac tgacgcagaa cattttgccg ggagtgccgt
     3001 actgggctct cgcaattttc tacgcaacag cttttacggt gctcaacctt cgaggcgtga
     3061 agacttccgc acgaatcaac gacgtgctgg ccgccggcat gaccatcgtg gtgatcgtat
     3121 ttttcggatt tgtgatccgg tttttgctgg gcttgcacga atatggggcg gggttctttt
     3181 cgcggccgtt ttacaatccg gccacattca atctgaattc cattttccac ggtacttcga
     3241 tcgccgtgct gacgtacatc ggttttgatg ccatttcgac gttctcggag gaagtagaga
     3301 acccacgtag gaacatcctg ctggccaccg tgctggtgtg cgtggtgacg ggagttttgt
     3361 cttcgctgga agtatatgcg gcgcagctcg tgtggggctc aaggccattt cccgcggaac
     3421 aggtggaatc ggcgtttccg ctggtggcga agcaggtggg cggctttttc ttgtttcagc
     3481 tgctgaattt caccattctg atcgccaata ttggctccgg gatgggcgcg cagttggctg
     3541 ccgcgcggct gctttacggc atgggccgca acgacgcgct gcccaagagt tttttcggca
     3601 agattgaggc gaagcggcag attccagcga acaacgtgat tttgatcggc gcaatcgcac
     3661 tggtgggctc gttttttctc acttacgaac ggggcgcgga gatgctcaac ttcggagcgt
     3721 tcatcgcctt catgggcgta aacgcagcgg cttttgtgcg ttacttcctc cgcgcggagc
     3781 ggaaaaagat atggccggtg ctttggccgc tgctcggctt tggaatttgc ggctacattt
     3841 tcctacagct cagtatgccg gcaaaa
//
LOCUS       DASYVO010000476         9506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_149786, whole genome shotgun sequence.
ACCESSION   DASYVO010000476 DASYVO010000000
VERSION     DASYVO010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9506
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149786"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1210
                     /gene="prmC"
                     /locus_tag="VGR03_16320"
     CDS             242..1210
                     /gene="prmC"
                     /locus_tag="VGR03_16320"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV1995899.1"
                     /translation="MSASESHIDVRTALKQGLAQLRDANVPSFTITAELLLLHALGRD
                     RTWLYAHPEEEISAADAQRFFALIARRANGEPTQHLTGKQEFWGLEFEVTRDVLIPRP
                     ETEHVIEVALDRLAIRELCAGRKQTLTGEGLQIADIGTGSGCIAIALAKELPGATIYA
                     TDVSSAATAIAQRNAKRHNLAEMIRFLEGNLLDGVSPLLDLIVSNPPYVGRREAETLA
                     REVRDHEPEIALYGGEEGYELYAALIAQAAAHLKPGGILVLELGHNSFPAVQPLLDAP
                     TWTNACVTNDLAGIPRVIAAEKGWSELSVSLDFRKTSDEHDRRKIS"
     gene            1396..2646
                     /gene="dinB"
                     /locus_tag="VGR03_16325"
     CDS             1396..2646
                     /gene="dinB"
                     /locus_tag="VGR03_16325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV1995900.1"
                     /translation="MPDLARFRAKHGPLGERSAEATASILHVDMDAFFVSVELLDRPE
                     LRGKPVVVGGRPDQRGVVSAASYEARKFGIHSAMPLRTASRLCPDAIFLDGHHSKYAE
                     WSDRVAGILAKFSPIVEMVSIDEAYLDLAGTERLHGPPLAAADKLLRMITQTTALPCS
                     GGLAATRLVAKVASDQAKPRGLVWVPAGSEQRFLATLPVRKIPGIGEVTERALRALGI
                     ETVEQIAAVPLEKLENIFGQWGMALYRKSRGGDSYEFVIDAEPKSISHNHTFGEDTGD
                     VDALSAMLSHLSQKACKRLREAGLATRTLTLTIRYAGFETHTRSKTLGKPTRLDFDIF
                     AVFQELFRVHRDVKRKVRLLGVSLSGLTHGSEQLDLLDTERRAKLEKLTTATDRLRDR
                     FGFGKVQFGGSLRRDEPGHDRDDS"
     gene            2703..3860
                     /locus_tag="VGR03_16330"
     CDS             2703..3860
                     /locus_tag="VGR03_16330"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEV1995901.1"
                     /translation="MSAPPTPGVSSPTRSPNVIERVRTFWQRISEGRQIDELWSHFTA
                     DARASYGFYMKESDAEELQGRRGIRRWFRIAKILFWSLLMKMTPARRVLLLVSLVLLV
                     MSGPKFRFTNNFTFDVNFESVAALGFLLLLSLELADKVTMKRDLEIAREIQTWLVPSQ
                     PPQVPGADIAFATRPQNSVAGDYYDAFYPNVAEPERLMLVIADVAGKSVPAALLMATL
                     QASLRTIAGEHAPLADLVARLNRYASAHSLNGLRFTTAVLGEYNPVSRKLTYVNAGHN
                     APILRRANGSIEILDVGGLPLGIQSDEEYETASLELNPGDALIFFTDGVVEAFNQAGE
                     DFGNDRWLSAIHNLPDWDAQQTLQFLMKRVDEFVGATRQSDDITCLVFRSR"
     gene            3942..5273
                     /locus_tag="VGR03_16335"
     CDS             3942..5273
                     /locus_tag="VGR03_16335"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV1995902.1"
                     /translation="MANSICETLRRGGYQALLVGGCVRDLLLGREPADYDVTSDATPA
                     HVMRLFPESVAVGAQFGVILIPRDGLKVEVATFRSDMGYSDGRHPDSVVYSNTPQEDV
                     QRRDFTINGLLMRPDSGEVLDYVGGQADLKARVIRAIGEPDRRFTEDKLRMMRAVRFA
                     ARFGFEIEAETFRAVRRHVSEIHQISPERLREELTKILTEGAARRAFELLDETWLLQQ
                     VLPEIGAMKGVEQPPQYHPEGDVWIHTRMMLEGLPADASPTLAWGVLLHDVGKPPTFQ
                     SAAETGDRIRFNNHVEVGVRMAEAICKRLRFSNEDTEQILALVDNHMKFGAVEEMRAS
                     TLKKFVRLPHFEEHLALHRLDCLSSHRHLDSYEFVTRFLAVTPPEQVRPERLLTGDDL
                     QTMGFRPGPLFAEILRALEDAQLEGQITTREEAAQYVMKRFTSKERRASSD"
     gene            5524..7950
                     /gene="lon"
                     /locus_tag="VGR03_16340"
     CDS             5524..7950
                     /gene="lon"
                     /locus_tag="VGR03_16340"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEV1995903.1"
                     /translation="MAEKKFVVPEILPILPVRDTVLFPGAVLPLTVGRESSLSLVNSL
                     QGEEKLLGVVAQLDPRVEDPAAADLHKLGTLAKVHKTVKMPNGNVVIFLEGLQRIQVL
                     DLIGLRPFLQARVQAEPDIIGEADTELEALQRNAQDLFRDVVSHSPQLSDDLQSVAMN
                     IDNPGRLTDFIAGTLPSLSTLLRQELIETASVRKRLETLIRELSKELEVLELRSKIHD
                     QVQEQVSQNQREYLLREQMKAIQKELGESDDSMQEIDELRKKVEEAAMSAEAKKECDR
                     ELKRLSKMTPASAEYMVSRTYLEWMTSLPWSKSSGSSEIDITKAHEILDEDHYDLQKV
                     KERILDYLAVKKLQPGMKGPILCFVGPPGVGKTSLGKSIARSLGRKFVRIALGGMHDE
                     AEIRGHRRTYIGALPGQVIQGLKRGETNDPVMMLDEVDKLGRDFRGDPSSALMEVLDP
                     EQNNSFRDHYLDVPFDLSKVLFIATANWMDPIPEPLRDRMEIIELPGYTGEEKLHIAH
                     KYLIPKQTAENGIKAGEQIEFTDEGLREIIHSFTREAGVRNLEREIATITRKQARRIA
                     EGKMEKMVVTPEVVREFLGVPKFRTEKEVEERVKRPGVAVGLVWTPVGGDIIFIEATR
                     MRGGKQFTMTGHLGEVMQESMTAALTWTRANCERYGIDPDFFRKHDIHIHVPSGAVPK
                     DGPSAGAAMVTALVSLLSGRAVKNRLAMTGEMTLSGIVLPIGGVKEKVLGAKRAGIKE
                     VLLPADNEPNVVADLTPEILGDIKITYVRTLDEVLEHALQKEAVTPPIVPQPEPKPKR
                     AGPESPRAIH"
     gene            8099..8686
                     /locus_tag="VGR03_16345"
     CDS             8099..8686
                     /locus_tag="VGR03_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995904.1"
                     /translation="MARKKPDAIDAPEELAKRISEALLRERVVPRFVDSYVVENGRHA
                     LQVHASLYRDLLALLQREALLALTVRALAIVCNEPQPAGKSKPRPMLRRDATAFRRKF
                     LASLTRQQGWTAGDALDFQRDLQMYEELLARAAATHRRRKPFEAADHPFVDRCAFLLD
                     SSFMEKARLAASRALTGIEQLATALTQQAQSRGHH"
     gene            complement(8707..>9506)
                     /locus_tag="VGR03_16350"
     CDS             complement(8707..>9506)
                     /locus_tag="VGR03_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995905.1"
                     /translation="ALRLASLVFVLELLICLCRAHFVFTLDTIGLFIFSVSTGLFVSC
                     FIWILYLALEPYVRRNWPQTIISWSRLLGGKLRDPLVGRDLLFGVLLGTAWVLVFEVG
                     AFFDIRAGARPQLSSTEYLVGMRETAAISLINLVNSIQSTLVFFLVLVFLRVLVKNRW
                     VAAAIFVLLFATPKLLGSDHPFIEGPVWAVIYGIAAIAVVRFGLIVLAIGIVLANVLL
                     NVPYTMDFSTWYAARSLCVVLAFVVMAIWGFYTSLAGQRLWKDELFE"
BASE COUNT         2106 a         2821 c         2752 g         1827 t
ORIGIN      
        1 cttcatcagc taagcgaggt gatggaagga aagctcgccc cgctcgtcga tggcctcgtc
       61 tcgcactttg aatcggagcg cctccagcag gagctcgcca gcgcgtgatg tttctccgct
      121 cggtgaacac gtcggcgatg cacatctctt tctcgtttcc gctgtttttt cttctcggtg
      181 ccctctgcgt ctctgcgccc tctgcgttaa ttctttcgtt tttttcttgc cgcgtgccgc
      241 gatgagtgcc agcgaatcgc acatcgacgt tcgcaccgcg ctgaagcaag gactcgccca
      301 actccgcgat gcgaacgtcc cttccttcac gatcacggcg gagttgttgc ttcttcacgc
      361 gctgggccgc gaccgcactt ggctctacgc gcatcccgaa gaggaaatct ccgccgccga
      421 cgcccagcgc ttcttcgcac tgattgcgcg gcgcgcgaat ggcgaaccga cacagcacct
      481 caccggcaaa caagaattct ggggcctcga atttgaagtc acacgggacg tcttgatccc
      541 ccgccccgaa accgagcacg tcattgaagt cgcgctcgac cgtctcgcaa tccgcgaact
      601 ctgtgctggc cgcaaacaaa cactcaccgg cgaaggcctg caaatcgccg acatcggaac
      661 cggctccggc tgcattgcca ttgcgctagc caaggaactc cctggcgcga ctatttatgc
      721 caccgatgtt tcatccgccg ccacggccat cgcgcaacgc aacgccaaac gccacaactt
      781 ggccgagatg attcgcttct tggaaggcaa tcttctggac ggtgtgtcgc cgctgctcga
      841 cctaatcgtg agcaatccac cttacgtcgg gcgaagggaa gcggaaacgt tggcacgcga
      901 agtccgcgat cacgaaccag aaatcgctct ctacggcggc gaagaaggct acgaactcta
      961 cgccgctctc atcgcgcaag ccgccgcgca tctcaagccc ggtggcatcc tcgtcctcga
     1021 actcggccac aacagcttcc ccgccgtcca accgctcctc gacgcaccca cctggacgaa
     1081 tgcctgcgta accaacgacc tagccggcat cccccgcgtc atcgccgccg aaaaaggttg
     1141 gagcgaatta tctgtctcgc tagacttcag aaagacatcc gacgaacatg accgccgaaa
     1201 aatatcttag gaagttcaca cgacgcgcac ccttgcttga gaagattcgg cacagcgcgg
     1261 aagccaaggg aattgatcgt ctttcactcc gcgagataaa tgctgagata aaggcctatc
     1321 gtcgaaaacg cagcaagaag gattaacagt tcacatcttc agttgacccg gccacaaaag
     1381 agttaggcta aggacatgcc ggacctcgcg cgattccgag ccaagcatgg gccgctcggc
     1441 gagcggtccg cggaggcgac agcgtccatc cttcacgtgg acatggacgc tttcttcgtc
     1501 tccgtggaat tgctcgatcg gccggagctg cgcggcaagc ccgtggtcgt gggcgggcgg
     1561 ccggatcagc gcggcgttgt ttccgccgcg agttacgaag cgcgcaagtt cggcattcat
     1621 tccgcgatgc cgctgcgtac tgcgagccga ctatgtccgg acgcgatttt cctggacggt
     1681 catcattcga aatatgcgga gtggagcgac cgggtcgcgg gcatcctcgc gaagttttct
     1741 cccattgtgg aaatggtttc gatcgacgag gcctacctcg acctcgccgg caccgaacga
     1801 ttgcacggac caccactcgc ggcagctgac aagttgcttc gaatgatcac tcaaacaacg
     1861 gcgcttccct gctccggtgg cctcgccgcg acgcgcctgg tggccaaagt cgcttccgat
     1921 caagccaagc cgcgcgggct cgtctgggtt cctgcgggca gtgagcaacg attcctcgcg
     1981 acgctgccgg tgagaaaaat tcccggcatc ggcgaggtca cggagcgcgc gcttcgcgca
     2041 ctcggcattg agaccgtaga acagatcgcg gccgtaccac tggaaaaact cgagaacatc
     2101 ttcggccagt gggggatggc actctatcgc aagtcgcgcg gcggcgattc gtacgaattt
     2161 gtgatcgacg ccgagccgaa atcgatttcg cacaatcaca cctttggcga agataccggc
     2221 gacgtggatg ccctgtccgc catgctcagc catctttcgc agaaagcctg caaacgcctg
     2281 cgcgaagccg gtctcgccac gcgaacgctc acgctgacga ttcgctacgc cggcttcgag
     2341 acgcacacgc gctccaaaac gctggggaaa cccacgcggc tcgatttcga catttttgcc
     2401 gtgtttcaag aactttttcg cgtccatcgg gatgtgaaac gaaaagtccg gctgctcgga
     2461 gtcagcctca gcggccttac tcatggcagt gaacaactcg atttgctcga tactgagcgc
     2521 cgcgccaagc tggaaaagct caccaccgcc acggaccgtc tccgcgaccg ttttggtttc
     2581 ggcaaagtgc aattcggcgg ctcgctccgc cgcgatgaac ccggacacga ccgcgacgat
     2641 tcctaacttc cagcgaatcc cagccggatg ccctatactt tatctttcat gagcgatcat
     2701 ccatgagtgc gcctccaacc cccggtgttt cttctcccac tcgttctccg aacgtgatcg
     2761 agcgcgtgcg gactttctgg caacgtatca gcgaaggccg ccagattgac gaattgtggt
     2821 cgcacttcac cgccgatgcg cgcgccagct acggcttcta catgaaagag tcggacgcgg
     2881 aagagctgca aggccgtcgc ggcatccggc gctggtttcg gattgccaag atcctcttct
     2941 ggtcgttgtt gatgaagatg acgccagcgc ggcgagttct gctgctggtg tcgctggtct
     3001 tattggtgat gtcgggaccg aagtttcggt tcaccaacaa ttttacgttt gacgtcaatt
     3061 tcgaatccgt cgccgccttg ggtttcctgc tcctgctgtc gttggagctg gccgacaagg
     3121 ttacgatgaa acgcgacttg gaaatcgcgc gggagattca gacctggctg gtgccgtcgc
     3181 aaccgcccca agttcctggc gcggatattg cgttcgcaac gcgcccgcaa aattcagtcg
     3241 ccggagatta ttacgacgcg ttttatccga acgtcgcaga acctgaaagg ttgatgctgg
     3301 tcatcgcgga tgtcgctgga aagagcgtac ccgccgccct tctgatggcc acgttgcagg
     3361 ccagtctgcg aacgatcgca ggagagcatg ccccgctcgc agatctcgtg gcacggctga
     3421 accgttacgc cagcgcgcac agtctcaatg gactgcgctt cacgacggcg gtcctcggtg
     3481 aatacaatcc cgtgtcgagg aaactcactt acgtcaacgc ggggcacaac gcgccgatcc
     3541 tccggcgcgc aaatggttct attgaaatcc tggacgttgg cggcttgccc ctgggaattc
     3601 aatccgacga ggagtatgaa acggcctcgc tggaactcaa tccgggcgat gccttgattt
     3661 tcttcaccga cggagttgtc gaagctttca accaagcagg ggaagacttc ggaaatgacc
     3721 gctggctcag cgccattcac aatctgccgg attgggacgc tcagcaaact ctccagttcc
     3781 tgatgaagcg cgtggatgaa ttcgtcggcg ccactcgaca gtccgatgac atcacctgcc
     3841 tcgtcttccg cagccgatag tccgccgccg aaactttccg gaacaccaat ctcccttcgc
     3901 agcccggtct gtcgcataat cacagcgtga ccccacggga aatggccaac tccatctgcg
     3961 agacactgcg ccgcggcggt tatcaggcgc tgcttgtggg cggatgtgtg cgcgacttgc
     4021 tgctcggccg cgagcctgcc gattacgacg tgacctccga tgcgacgcca gcgcatgtga
     4081 tgagactgtt ccctgagagc gtggcggtcg gcgcgcaatt cggcgtaatt ctgattccgc
     4141 gcgatggatt gaaagtcgaa gtagccacct tccgttccga tatgggctac agcgacgggc
     4201 ggcacccaga cagcgtggtt tattcaaaca cgccgcaaga ggacgtgcag cgccgcgact
     4261 ttaccatcaa tggtctgctg atgcgccccg acagcggcga agtgctcgac tacgtcggcg
     4321 ggcaggccga cctcaaagcg agagttattc gagccatcgg cgagcccgac cgccgtttca
     4381 cggaagacaa actacgcatg atgcgcgcgg tgcgattcgc cgcgcgtttc ggattcgaaa
     4441 tcgaagccga aacatttcgg gcggtacgcc gccacgtcag cgagatccac cagatctcgc
     4501 cagagcgact ccgcgaagaa ctcactaaaa ttctcaccga gggcgcggcc cggcgtgcgt
     4561 tcgaattgct ggatgaaacc tggctgctcc agcaagttct tcccgaaatc ggggcgatga
     4621 aaggagtcga acagcctccg caatatcatc cggaaggaga cgtgtggatt catacgcgaa
     4681 tgatgctcga agggctgcct gcagacgctt ccccgactct ggcctggggc gttcttctcc
     4741 atgacgttgg caagccacca acgttccaat ccgccgcgga gacgggggac cgcattcgtt
     4801 tcaacaatca cgtcgaagtc ggtgtgcgca tggccgaggc catttgcaag cgattgcgct
     4861 tctccaacga agacaccgag cagatccttg cgctggtgga caatcacatg aagttcggag
     4921 cggtcgagga aatgcgcgcc tccaccctga agaagtttgt gcggctcccg cactttgagg
     4981 agcacctggc gttgcatcgg ctcgactgtc tttccagtca ccgccacctc gattcctatg
     5041 agttcgtaac gcgattccta gcggtgaccc ctcccgagca agtccgcccg gagcgattgt
     5101 tgacgggcga tgacctccaa acaatggggt ttcgacccgg ccctctattt gctgagatac
     5161 tccgggcact tgaggacgcc caactggagg ggcagatcac gacacgagaa gaagcagcgc
     5221 agtatgtcat gaagagattc acttcgaagg agcgaagggc ctcaagtgat tgagcgagta
     5281 gtcgatagga aaaatgcagc atagcgactg tctgcgcttc ctcataactt ctgtaatatg
     5341 ctgattataa acgatttatt ctacattttg tagtccagca cagttttagg tagaatgtga
     5401 gtgaaaggta ctaagatttt gtgctagcaa cttttatact tttgtgcacc ctttccgaca
     5461 cgcttgcatc taaaatcgta tcgcgcagct ctattctggc tgcggcggta accagggggc
     5521 atcatggctg agaaaaaatt tgtggtgcca gagattcttc ccatcctgcc ggttcgtgac
     5581 accgtcctgt ttccgggcgc tgtcctgcct ttgaccgtgg gacgagagag ttctctgtcg
     5641 ttagtgaata gcttgcaagg cgaggagaag cttctgggag tggtcgcgca gctggatccg
     5701 cgagtagagg atcctgcggc cgctgacctc cacaaactcg ggacgttggc caaggtgcat
     5761 aagaccgtaa aaatgccgaa cggcaacgtg gtcatcttcc tggaaggcct ccagcgcatt
     5821 caagtgctcg acctgatcgg cttgcgtcca tttctgcagg cgcgcgtgca ggcggaaccg
     5881 gacatcatcg gcgaagcaga tactgaactc gaagcgcttc agcgaaatgc gcaggatctt
     5941 ttccgcgacg tggtaagcca ctcgccgcag ctctctgacg atctgcaatc cgtggccatg
     6001 aatatcgaca atccgggccg cctgacggat ttcatagcag gcactctgcc gtcactttcg
     6061 acgctgctgc gccaggaatt gatcgagacc gccagcgtgc gcaagaggct ggaaacgctt
     6121 atcagggaac tatcgaagga gctcgaggtc ctggagcttc gctcgaaaat ccacgatcaa
     6181 gtgcaggaac aggtcagcca gaaccagcgc gagtacctcc tgcgcgaaca gatgaaggcg
     6241 atccagaaag aactcggcga atccgacgat tcgatgcagg agatcgacga gcttcgcaag
     6301 aaagtggaag aagcggccat gtccgccgaa gctaagaaag agtgcgaccg cgagctgaag
     6361 cggctgtcaa aaatgacgcc ggcttccgcg gagtacatgg tttcgcgaac ttatctcgaa
     6421 tggatgactt ccctgccgtg gagcaagtcc tcgggcagct cggaaatcga catcacaaag
     6481 gcgcacgaga ttctggacga agaccactac gatttgcaga aggtgaagga gcgcatcctc
     6541 gattacctgg cggtgaagaa acttcagcca ggcatgaagg ggccgatcct ttgcttcgtc
     6601 ggacctccag gcgtgggtaa gacttcgctt ggcaaatcga tcgcgcgttc gctgggacgc
     6661 aagttcgtgc gcatcgctct tggcggaatg cacgatgaag ccgaaattcg cggccaccgc
     6721 cgcacctaca ttggcgcgtt gcccggacag gtcattcagg gcctgaagcg cggggaaacg
     6781 aacgacccgg tgatgatgtt ggacgaggtg gacaagctcg ggcgcgattt ccgcggcgat
     6841 ccgtcatcgg cactgatgga agttctcgac ccggagcaaa acaactcgtt ccgcgatcac
     6901 tatctcgatg tgccgttcga tctctcgaag gtgctgttca tcgccacggc gaactggatg
     6961 gatcccatcc ccgagccgct gcgggaccgc atggagatca tcgaattgcc cggctacacg
     7021 ggagaagaga agctgcacat cgcgcacaag tacctgatcc cgaagcagac agccgagaac
     7081 ggcataaaag cgggagagca gatcgaattc accgacgagg ggctgcggga aatcattcac
     7141 agcttcacgc gggaagcggg cgtgcgcaac ctggagcgcg agatcgcgac catcacgcgc
     7201 aaacaagcgc ggcgcatcgc cgaaggcaag atggaaaaga tggtcgtcac accggaagtg
     7261 gtccgcgaat tcctgggcgt cccgaaattc cgcacggaga aggaagtcga ggaacgcgtc
     7321 aagaggcccg gcgttgccgt cggactggtc tggacgccgg taggcggcga catcatcttc
     7381 atcgaagcga cccgcatgcg tggcggcaag cagttcacca tgaccgggca tctgggcgaa
     7441 gtcatgcagg aatcgatgac cgcggcgctg acatggacgc gcgctaattg cgagcgctac
     7501 ggcatcgatc cggacttctt ccgcaagcat gacattcaca tccatgtgcc gtcgggtgcg
     7561 gtgccgaagg acggcccgtc cgccggtgca gcgatggtga cggcgttggt gagcttgttg
     7621 agcgggcggg cggtgaagaa ccgcctggcg atgaccggcg aaatgacgct ctcgggaatc
     7681 gttctgccta tcggcggcgt gaaggaaaaa gtactcgggg cgaagcgggc gggaatcaag
     7741 gaagtcctgc tgcctgcgga caacgagccg aatgtcgtgg cggatctcac tccagagatt
     7801 ctcggcgata ttaagattac ctacgtgcgc acgctggacg aagtgctgga gcacgcgctg
     7861 cagaaggagg cggtgacgcc gccgatcgtt ccgcagcctg agccaaagcc aaaacgcgcc
     7921 ggcccggaga gccccagggc gattcactaa gcgtttgttc caagtgcgaa gcaagtcgta
     7981 gccaagtgac agctgactcg tgactgctgg aagagcctgg cgaagtttgc cgggcttttt
     8041 catttccgat acgcggcacg ggcaaggtac taactcctgc taacatgttt cccggaacat
     8101 ggcgcgtaaa aaaccagatg cgatagatgc accggaagaa ctcgccaagc gaatttcgga
     8161 ggcgctgctg cgtgagcgcg ttgtgccgcg gtttgtggat tcttacgtcg tcgagaacgg
     8221 ccgccatgcg ctgcaggtcc acgcctcgct ttaccgcgat cttctggcgc tgttgcagcg
     8281 agaggcgctg ctggctttga cggtgcgggc cctggcgatc gtctgcaatg agccgcagcc
     8341 tgccggcaaa tccaaaccgc gtcccatgct gcggcgagat gccacggcgt tccgcaggaa
     8401 atttctggcg tcactgacgc gacagcaggg ctggacggcg ggcgacgcgc tcgattttca
     8461 gcgagacctg caaatgtatg aagagctgct ggcgcgtgcc gcggcgacac atcgccgccg
     8521 caagcccttt gaagcggcgg accatccctt cgtcgaccgc tgcgctttcc tgctggattc
     8581 gtcgttcatg gaaaaggcgc ggctggcggc cagccgcgcg ctaacaggca tcgagcaatt
     8641 ggccacggct ctaacgcaac aagctcaatc gcgcggccat cactgattga gaagaaacga
     8701 tccttcttac tcgaagagtt cgtctttcca gaggcgctgc cccgcaagtg atgtataaaa
     8761 gccccagatg gccatgacga caaaagcaag cacgacgcaa agagagcgtg ctgcgtacca
     8821 ggttgaaaaa tccatcgtgt aagggacgtt cagcagcaca ttggctagga caatcccgat
     8881 tgccaaaacg atcaaaccga atcgcaccac ggcaatggcg gcaataccgt agataaccgc
     8941 ccaaacgggc ccctcgataa agggatggtc gctccccagg agcttcggtg tggcgaaaag
     9001 cagaacaaat atcgccgcgg ccacccagcg gttcttgacc agcacgcgca ggaacacaag
     9061 gaccaaaaaa aagaccaggg ttgactgaat cgaattcacc agattgatca acgaaatcgc
     9121 tgcggtctcc cgcattccca caaggtattc tgtactgctg agttgcggcc gggctcccgc
     9181 ccggatgtcg aaaaaggcgc cgacctcaaa taccagtacc cacgccgttc cgagaagtac
     9241 tccaaacaaa agatcgcggc cgaccagcgg atcacggagc ttgccgccca gcaagcggct
     9301 ccaagagatg atggtttgag gccagttccg ccggacgtag ggttcaagcg cgagatagag
     9361 aatccaaata aagcacgaaa cgaatagccc ggtactcacg ctaaatatga acagaccgat
     9421 ggtgtcaagt gtgaacacga agtgcgcacg gcacaagcaa atcaagagtt cgagaacgaa
     9481 taccaaggat gccagccgca acgcac
//
LOCUS       DASYVO010000477        16326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_191928, whole genome shotgun sequence.
ACCESSION   DASYVO010000477 DASYVO010000000
VERSION     DASYVO010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 16326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16326
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191928"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..1329)
                     /locus_tag="VGR03_16355"
     CDS             complement(259..1329)
                     /locus_tag="VGR03_16355"
                     /inference="COORDINATES: protein motif:HMM:NF024420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEV1995906.1"
                     /translation="MGRILGTPADKFGVYYGGRRNEAARKYQHTKMWGASAEEAFASV
                     KTAIVGLLRAAEKDQMAVVRASKISPMFKGKLLFLYFPDKYAPIYSPEHLRHFAAELN
                     LAGPFMGAVDIQRALMTYRASWPQLASEPECLYMRFLYDVFGYPAEEKGEDKASPELP
                     LLDDAVEGAEFIDLMPPLLSKEAHRGAANGKKPDYLKRLKQLERIGNRGEAIVVALER
                     KRLIQAGKAHLAKKVDRVSEHDDSLGYDILSFEEDGTDRAIEVKATSGKNLDRGFYIT
                     SNEVKKAAGLRNYYVYLVFLALSKNPRVLPVKHPGFKGKDFVLDPVVFHVTLPRVPDP
                     QILEPGAPSARGVQCCGTRTEN"
     gene            complement(1698..1841)
                     /locus_tag="VGR03_16360"
     CDS             complement(1698..1841)
                     /locus_tag="VGR03_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995907.1"
                     /translation="MKTKSQGTQPHWGNRAGKKYGKYRICDERFYSYWVPGLVELIFL
                     DWF"
     gene            complement(2585..4132)
                     /locus_tag="VGR03_16365"
     CDS             complement(2585..4132)
                     /locus_tag="VGR03_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995908.1"
                     /translation="MRTRLLTILITLTLTGGTLPARAQEAGKIVDQYVKTAGGGRALA
                     RIQTLTLEGTFTSDDGKTGTYTLDTKLPNRYYSELLVGERNLIEAYNGKSAWHQSATG
                     ELGTLVGPEGMQLEAAAQYYNSRLLNHKKSKIALRLVGHAQVRGRDALQLEVTTATGV
                     KRQVFFDPQTHLIVKEAATVGGVEEEILYDDYRTIDGVKLPYKVELHRGSDKYDISVT
                     RAAINGTVGERVFDFPIKSQVKLPDLKALFKEIDDNQKAIDKIKENYTGTRAEEETEF
                     ESSGKVKKREVTEYTFFYLNGEEVSTVVKKDGKPLSDTEQKKENEKTQKRIEEHQKRE
                     AKKELKEEKAKEEGKSDEKDEPGIEVFLRACQFVNPRRERFRGQDVLVFDFEPNPEFK
                     AHKLVERVVQKLAGVVWVDEKAHDVARLEAYFVGDMKIGGGLLANLQKGTSFVIEQAF
                     VNNEVWLPTYEEAHVGVRVLLVKGIKVNAVTRYSDYRKFNVETLSTIAKPKGASEAPA
                     DPPAKPQ"
     gene            complement(4259..5602)
                     /locus_tag="VGR03_16370"
     CDS             complement(4259..5602)
                     /locus_tag="VGR03_16370"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV1995909.1"
                     /translation="MKTTSIALRIALIFTAVCALPCLACAQSQLSTTYALTHAKIFTL
                     AGSTIEDGTLIIRDGKIAAVGVDLDIPAGAQVIDAKGLQVYPGLFDSITQMGLREIGA
                     VSATVDSAESGSFNPDVVAATAVSPSSEHIPVTRAAGITEVLAVPASGGMDSFGSAGV
                     IGGQASAINLAGWTIDEMLIRKSAAMVLNWPEIETQTFDFATFSRKEKPFSDAKQEYD
                     KQVNEITDWVERARHYSQVMEKSSPAKYDRDLKLEALVPVVRGELPLLIFADRSREIR
                     NAVEFCDKERLRMILAGGLEAYKVEDLLRSKGVPVILRPMLTLPLDEDDPYDRLLSQP
                     AELAAAGVKFAFASFDNSFARRLGQNAANAVAYGLPYDEALKAVTLYPARIFGLDDQI
                     GTLEPGKLANLIVTNGDPLELTTDVKYLFIKGQLTSMDNRHLRLYEKYSKRPKAQ"
     gene            complement(5599..7170)
                     /locus_tag="VGR03_16375"
     CDS             complement(5599..7170)
                     /locus_tag="VGR03_16375"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV1995910.1"
                     /translation="MRISNVLNAGRLRTSFLYFLCSLCIIYFSVPAHAATPTDAPAVI
                     VIRNATILTVSHGTIEHGSILIKDGKIAEVGQSINAPRDAQVIDAAGQFVMPGIIDCH
                     SHIAAESINEGSVSVSSMVNMAEILNPDDIDIYRDLAGGVTSANILHGSANSIGGQTL
                     VIKLRWGQPAAKLPFEGALPGIKFALGENPKRSNFSIPGQPRRYPSTRMGVEETIRGA
                     FSEARDYKKAWEGYNKRLAVGEKNLIAPRRDLRLEPLVEVLEGKRYVHSHCYREDEIL
                     MLLRVAKEFGFKVRTFQHILEGYKVADELAAAGVGASTFSDWWAYKVEAYEAIPYNAA
                     LMTRRGVVVSINSDDAEEATHLNQEAAKSMKFGGLSHDEALKLVTLNPALQLGIDKRV
                     GTIDVGKDADLAIYNHDPLSAYAVVQKTLIDGRVYFDRQRDIAERSEREKEKKALIEK
                     FKKAAEKKAEEKKAEEKKPEDKKPDATVPIGQKPSEKKPEEKKPEEKKPEDKPKPPQS
                     SGNDSATVVLPEGAL"
     gene            complement(7167..8768)
                     /locus_tag="VGR03_16380"
     CDS             complement(7167..8768)
                     /locus_tag="VGR03_16380"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV1995911.1"
                     /translation="MAQGGEPQYFAIRGARVVPVSGPPIENATIVVSRGLITAVGKDI
                     AIPLEAWVIDGKGLTVYPGLFDSFTDVGIPVAPPASGEGGPRRTQEGARGPEDRPAST
                     PWRSAADEVSLSDKRIETWRNAGFTTVVSAPKGGIFPGQAAVLDLAGERAGDLVVKSP
                     VAIPVVFQTSGGFGGGFPNSIMGVLAYVHQVWLDTEWSTRAQATYEKNPRSVARPRYD
                     RTEAALANALEDHALVLIPANNSVQLRRALELVDRWHVNGAIYGGQMAYEVAPEIAAK
                     KLPVLVNLKWPETEKDADPEDKPSLRALQFRDRAPSSPVALAKAGVKFAFYSGGITAP
                     KDTLKAAKKSIDAGLAPDAALRALTLSAAEIFGVADRLGSIEIGKIASLVVADGDLFD
                     EKTKIKMVFVDGRRFEVREPEKPKEPPKGDLTGKWKLSYTTPDGPEESTADLAMEKDG
                     TISGTVTSKRGTTSLISGYLSVDKFSFTINIPLEGSPADVTFSGTFDGTSLKGSISVQ
                     GFSLDFTGVKPGAASATMASLGGAQ"
     gene            9080..9424
                     /locus_tag="VGR03_16385"
     CDS             9080..9424
                     /locus_tag="VGR03_16385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1995912.1"
                     /translation="MGERADVWQGTLALMVLKTLQALGPLHGYGIARRIEQTSGDLLA
                     VNYGTLYPALLKLEQEGYISSEWGTSDNNRKAKYYKLTRAGRKQLDKEAREWAQTTEI
                     LARFLAPGKESA"
     gene            9421..12081
                     /locus_tag="VGR03_16390"
     CDS             9421..12081
                     /locus_tag="VGR03_16390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1995913.1"
                     /translation="MRRLRALFIRLGGLFREDRQEREFTAEMESHLQMHIEDNLRAGM
                     STAEARRQALIKLGGMEQTKENYRDRRGLPLLETLFQDLRYAARMLLKNPGFTAVAVI
                     TLALGIGANTAIFNVVHATLLQPLPIRHASRLVVIWVNNLEHGWSRIGPTGQDYLDWK
                     EQAKSLDDIFLFEHGTGTVTGPGEPEQVAGLRVTTNFGGFFGIKPVFGRTFRLEEAGG
                     RHNFVILGNSYWRRRFSSDPSVVGRGMTLNGEEYTIIGVLPAEFAALFPADVVVPFDT
                     AWVKRVDTDLGVFGRLKPEATLNQATREMSVIAERIAIARPSRKGFGVVLVPLESVRL
                     EYLRPALLVLLGAVGFVLLISCANVANLLLARSVMRQREMAIRMALGAGHLRLIRQFL
                     AESTLLSLLGGAVGSLLALWSTELLKIFLPSRIPVPNAADAVMLPRIHMSGAAFAFTI
                     VISLLTGIIFGLIPALQSLKCNVNESLKEGGRGFLSGPRGHRTRSALVIAESALAFVL
                     VIGAGLMIKSFWRLLEANPGFHPDHLLTLRIKLPADAKDSKYREPRQQAATFQRFLAS
                     VEAVPGIQSAAFAEIVPLSQDDMDMGYFTLKEGPLRPPGEHLAADYRDVTSNYFAAMG
                     IPLIAGRIFTEQDNLDRPRVVVIDEPLARRFFTGEDPIGKHLQIPDYTRPAREIVGVV
                     GGVRDTGFDQPPRPTIYFPSLQSPDQTMSLVVRTTLPPSAILPAIKNAIWSVDKNQPV
                     FEVRSMDEIISGIVSAQHLAFLLLGVFAFLALALAAIGIYGVTSYVVSERTHEIGVRM
                     ALGAQPRDVSRVVLGLGARLAGIGVIGGVLAALALTRLLSSLLFGVSATDPFTFVGVA
                     ILLTLVALAACYIPARRAMRVDPIVALRYE"
     gene            12140..14794
                     /locus_tag="VGR03_16395"
     CDS             12140..14794
                     /locus_tag="VGR03_16395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1995914.1"
                     /translation="MKWLRGLLLRLGELFGKERRERELAAELESHLQMHIEDNLRARM
                     STAEARRQALIKLGGMEQTKENYRDRRGLPFLETLFQDLRYAARMLLKNPGFTVVAVL
                     TLAIGIGANTAIFSLVNGVLLRPLPYREPARLTIVWGKDDQGKPGNVGYATYVDWKAQ
                     NKSFEELALYRSWSPVLQTNEPEQLSGLRVTNNYFRALGVHPQFGRDFRPEEDIPAAS
                     HVVILSHGLWQRRFNSDQSIIGRAISLNATTYLVAGVLPADFQSLISMDPHGGPVDIW
                     GVLGYDASLPWACRTCQHLVAIGRLRPGVAFTQATAEMDTVSSALWKAYPKEYSAAGV
                     ILTPLREHLVGPVSTTLYILLGAVSFVLLIACANLANLLLARATHREREMAVRTALGA
                     ARGRIVRQLLVENCLLAALGAAAGLLLAYWTPQLLRVLGAGELPRLDEVKVDSHVLLF
                     TLGMALLTGILSGLAPALRLSKTNLHDSLKEGARGTSAGTGRRMRSLLVVSEIALSLT
                     LLVGAGLLLRSLSQVLGVSAGFDSNHVLTMRISVLGQRYNDEKNLRQFFTSALASIRA
                     LPGVQSAAVTSQVPLGGNGDRYGFHVEGKIHVNPELDESAERFCVSPGYLDAMRIRLL
                     RGRDISESDSSTAQQTLLISETTARSMWPGEDPIGKRVKLGGVDLPWWTVAGVVADVH
                     HDGLDIAPTMQFYVPHAQWPYPDSDMTFTIRTAGPPGTMASAAQQAIHALDPTQPLSR
                     VMPLADYVGLSVQGRRFSSILLAAFAAIALLLSALGIYGVTAYSIAQRTREIGIRMAL
                     GAQQKEVFALLLRQSVVLVVFGVALGVATSAALTRFLASMLFDVRPTDPATFVSVVSL
                     LVGIAALACWFPARRAMRVDPMVALRYE"
     gene            complement(14953..>16326)
                     /locus_tag="VGR03_16400"
     CDS             complement(14953..>16326)
                     /locus_tag="VGR03_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995915.1"
                     /translation="NYSSLGGSAAKSTGSTNAPADLTIKKLELKDGSIIVGSTNSQKR
                     STYDHVAVTATGVAITSQFPVTVTADLPSGGKFKLDGIVGPIDQADTALTPLTAKLNV
                     SSLNLASTGFLDPSMGLGGLLDLDGTLESKGGEAEIKGNVKLSNALLVAGGAPAGVPV
                     IVDFNTKYNLRKNAGVLNPSTLKIGSAAARLNGTYQSAGEATVANIKLSAKDMPAKDL
                     EAFLPALGIHLPKGASLSAGTLNTDLTLAGPMSKMVTTGTVGLFNGVIANFDLGSKMS
                     SVASLAGIKSGKDLNIEKFTSNLRVAPDGIKADNVLAVLPTIGNLTGAGTVDSKNALD
                     FKMLATLTKQASSAAGSAGAVANGAAGALGGLLGTVTGGGGAGGVGGCTSSSGGLKVP
                     FQIQGTTSDPKFIPDIGGLAKDLFKSELGCLGGRSTASIAKVPGQGQNPADAINALGG
                     LFKKKKP"
BASE COUNT         3128 a         4774 c         4730 g         3694 t
ORIGIN      
        1 caagagcggc atcacggatt tcgatgttca tcgctggttc cttgcgcgtt ggcgattatg
       61 gtctttctgc atacgagctg acgtgccaat aaacgccccc gatacaggcc aatatccagc
      121 agtttatcac gaaccaaagg ggcgtggacg ctcccgcggc ccccgcccgt catttcgaac
      181 gcatgtgaga aatctctctt tgcctctctt cgctcttccc atttctagtt tctgctttcc
      241 agttcctagt ttccgctttc aattttctgt ccgtgtgcca cagcattgca caccccgcgc
      301 ggatggcgca cccggctcaa gaatctgagg gtccggcacc cgcggcagcg tcacatgaaa
      361 taccactgga tcgagtacga agtctttacc cttaaagccg ggatgcttta ccggcaggac
      421 tcgcggattc ttgctcaatg ccaaaaacac gaggtacaca tagtaatttc ttagtcccgc
      481 cgctttcttc acctcgttgc tggtgatgta gaatcctcga tctagattct tgccgctcgt
      541 cgccttaacc tcaattgccc gatccgttcc atcttcctcg aacgagagaa tgtcgtaccc
      601 caggctatcg tcatgttccg agacgcgatc tacctttttt gcaagatgag ccttgccagc
      661 ctggattaac cgcttcctct ctagcgcaac gactatcgcc tccccgcgat tgccgatacg
      721 ctctaattgt ttcagacgct tcagataatc aggttttttg ccgtttgccg cgccacgatg
      781 tgcttccttg ctcaacagag ggggcatcag atctatgaac tcagcacctt caaccgcgtc
      841 gtcgagcaac gggagctccg gagaagcctt atcctccccc ttctcttccg ctggatatcc
      901 gaatacatca tagagaaaac gcatgtagag acattcaggc tcgctcgcaa gctgaggcca
      961 gctcgcgcgg tacgtcatga gtgcccgctg aatatccacc gcgcccatga atggcccggc
     1021 cagattcagc tccgccgcga aatgccgcag atgctccgga gagtagatgg gcgcgtactt
     1081 atccggaaaa tacagaaaga gcaatttgcc tttgaacatc ggcgagatct tgctcgcccg
     1141 gactactgcc atttggtctt tctccgctgc ccggagcaga ccaacgatag ccgtcttgac
     1201 cgaggcaaac gcctcttcgg cactagcgcc ccacatttta gtgtgctggt atttgcgggc
     1261 ggcttcgttt ctcctgccac cgtagtagac tccaaactta tcggctggcg tgccgagaat
     1321 ccgccccatg cgatccagct cccgctccac gcgatagcag aaggagcggt tgtcacggcc
     1381 ttttccgata acgtattcat cgagagtcag ccgggggatg cgcgaaacag catagtcagc
     1441 cacaaatcgt tgccgcagtt tttccaagtg cttccactcc gccgcatctg gcttcgcgag
     1501 aacaggcacg cactcggcaa tgcgaccggc catttccaag cgattcatgc ttcaccgtgc
     1561 tttcaattat gcgctccccg ctgcacacta gtacatcctt ggcccccgtc aatgggcgtc
     1621 cgcgaccggc gggtgggcca gcctccatac agccaaaagt attcccggaa gacttcccct
     1681 gcgccgcccg ctcacgatca aaaccaatcc aggaaaatca gctcgacaag gccgggaacc
     1741 cagtaggaat aaaatcgctc atcgcagatt ctgtactttc cgtacttctt cccggcacgg
     1801 ttgccccagt gcggctgggt gccttggctt ttagtcttca tattgcgatt tctgcatgcg
     1861 tagatcggca aaccacatcc tttcaacacc ttccgcaatc attcgaatta cacattaaat
     1921 aaacattgac agtcagtacc ttttgtgtta tagtgttgtt gtctttcacc atggcggaga
     1981 aaggagcgca tccctttgtc cgctacctct ctatcgttac ttcccaccac tcgcctctgc
     2041 atccaaattc ttttgtttca taactttccg gactctttcc tcatccgatt gcacaacagc
     2101 tcagaacaac tcatttcaaa tcaaacagtt ccgcactctt tgccgtcaca acgggggtgg
     2161 gtgggcacgg cttctctccc acgctccgcc tgtcccggtc aggtcgtggc acttcccgca
     2221 gagggggcat catgttgact agctatgctc ctcctccgtg ctcccggcgt ctcagaggca
     2281 atccctattc cgcatttttc ttcactgacc atcgaagctc cacctcagtc ggggagcgga
     2341 gatcccgacc tggtcgggac cgtccacgga tcactgctca ctttcttcta agttctttcc
     2401 ttgccacaca tccgaaaaac gcacccataa ctcctttgct tgccacacat ccaaaaacac
     2461 aggacttaaa gtcctgtgtt tgccacacat ccgaaaaaag tgggggtagg ggggcaaatt
     2521 gttaaccaga attgccggtc gtccgcccga acggagtctt ggaattatct atggagaaag
     2581 agtcttactg aggcttggcg ggagggtccg cgggtgcctc gctcgccccc ttgggcttgg
     2641 cgatcgtgga gagcgtctcc acattgaatt tcctgtagtc cgaatagcgc gtcacggcgt
     2701 tgactttaat tcctttcacc agcaacacgc gcacgccgac gtgggcctcc tcgtacgtgg
     2761 ggagccacac ttcgttgttc acaaacgcct gctcgatgac gaagctcgtg cccttttgca
     2821 gatttgccag caagccaccg ccaatcttca tgtcgccgac gaaatacgct tccagccgcg
     2881 ccacgtcgtg cgctttttcg tcgacccaaa cgactccggc aagcttctgc acaactcttt
     2941 ctaccagctt gtgcgctttg aattcgggat tcggctcgaa atcaaaaacc aaaacatcct
     3001 gtccgcggaa gcgctcccgg cgcggattca cgaactggca ggcgcgcagg aatacttcaa
     3061 tccccggctc atccttctcg tcgctcttcc cttcttcctt ggccttctct tctttcaatt
     3121 ctttcttggc ttcgcgcttc tggtgttcct cgatccgttt ctgcgtcttc tcgttttctt
     3181 ttttctgttc cgtgtcgctc agcggcttgc cgtccttctt caccaccgtg gatacctctt
     3241 ctccgttcaa gtagaagaaa gtgtattccg tgacctcgcg cttctttact ttgccgctgc
     3301 tctcgaactc ggtctcttcc tccgcgcgcg ttcccgtgta gttttccttg atcttgtcga
     3361 tggccttctg gttgtcgtca atctctttga acagggcttt caagtccggc aatttgactt
     3421 gcgacttgat cggaaaatca aacacgcgct cgccaactgt tccattgatc gccgcgcgtg
     3481 tcacgctgat gtcgtatttg tcgctcccgc ggtgcagctc aaccttgtac ggcaacttca
     3541 cgccatcaat cgttcggtag tcgtcataca ggatttcttc ctccactccg ccgaccgtcg
     3601 ccgcctcctt cacgatcagg tgcgtctgtg gatcaaagaa tacttgccgc ttcacgccgg
     3661 tggccgtggt gacctcgagt tgcagcgcat cccttccgcg cacctgtgcg tgccccacaa
     3721 gcctcaacgc gatcttactt ttcttgtgat tgagcagccg ggagttgtaa tactgcgccg
     3781 ccgcctccaa ttgcatccct tcgggaccca ccagcgtgcc cagctcgccg gtcgcactct
     3841 gatgccatgc ggacttcccg ttgtacgcct cgatcagatt cctttcgccc accagcaatt
     3901 ccgagtaata gcgatttggc agcttggtat ccagcgtgta cgtcccggtc ttcccgtcgt
     3961 cgctcgtaaa ggtcccttcg agcgtcaacg tctgtatcct ggccagtgcc cggccgcctc
     4021 ccgcggtctt cacgtactgg tccacaatct ttccggcctc ctgcgcgcga gcaggaaggg
     4081 ttccgccagt gagcgtcaat gtgatgagta tggtaagcag gcgtgtacgc ataaggttca
     4141 actgtatgaa cgcaactccg cgcaaaaagt ttctcggaat ttgagcctag gggcgcagct
     4201 tcatccctag cgtgagcgaa agaatgctaa gcccctacgt ttgcaaagtg cggtcaaact
     4261 attgcgcttt cggcctcttc gagtacttct cgtagagccg cagatgccgg ttatccatgg
     4321 acgtgagctg ccccttgata aagaggtact tgacgtcggt ggtcagttcg agcgggtcgc
     4381 cgttggtcac gatgaggttg gcgagtttgc ctggttcgag ggtgccgatc tgatcgtcga
     4441 ggccgaaaat ccgcgcggga taaagcgtga cggccttgag cgcttcgtca tagggcaagc
     4501 cgtacgcgac ggcgttcgcg gcattttgtc ccaaccgccg cgcgaaggaa ttatcgaagc
     4561 tggcgaaggc aaacttcacc ccggcagcgg caagctcggc gggctgcgaa agcaagcgat
     4621 cgtacggatc gtcctcatcg agtgggagcg tgagcatggg ccggaggatt acgggaacgc
     4681 ctttcgagcg gagcaggtcc tcgaccttgt aagcttccaa gccgccggcg aggatcatcc
     4741 tcagcctctc tttgtcgcaa aactcgaccg cgttgcggat ctcgcgcgag cgatcggcaa
     4801 agatcagcag aggtagctcg ccgcgaacga ccggcacgag cgcttcgagc ttcaggtcgc
     4861 gatcgtattt cgccggtgag ctcttctcca taacctggga atagtgccgc gcgcgctcca
     4921 cccagtcggt gatttcattc acctgtttgt cgtattcctg cttggcgtcg ctgaagggtt
     4981 tttcctttcg cgaaaaagtg gcgaagtcga aagtttgggt ctcgatctcc ggccaattca
     5041 gcaccatcgc cgcacttttc ctgatgagca tttcgtcgat ggtccagccg gcaaggttga
     5101 tcgcggatgc ttgaccgccg attacgcctg ccgagccgaa agaatccatg ccgccgctgg
     5161 cggggacggc gagaacttcc gtgatgcccg ctgcgcgggt gacgggaatg tgctcgctcg
     5221 aaggtgacac cgccgtggcc gcgacgacgt ccgggttgaa gcttccggac tccgccgaat
     5281 cgacggtcgc gctgaccgcg ccgatctccc gcaagcccat ctgggtgatc gagtcgaaca
     5341 atcccgggta gacctgcagt ccttttgcgt cgattacctg ggcccccgcg gggatgtcca
     5401 agtcgacgcc aaccgccgcg atttttccat cgcggatgat cagcgtgccg tcttcgatgg
     5461 tgctccccgc gagggtgaag attttcgcgt gagtcagagc gtaggtggtg gagagttgcg
     5521 attgcgcgca tgccagacag ggcagagcgc agaccgcggt gaagattaga gcgatccgga
     5581 gagcgatgga tgtagtcttc acagcgcacc ctcgggcaga accactgtcg ccgaatcgtt
     5641 gccgctcgat tgaggcggtt tgggtttatc ttccggcttc ttttcttccg gctttttctc
     5701 ttcgggtttc ttttcggagg gcttttgccc gataggcacg gtggcatcag gcttcttgtc
     5761 ttctggcttt ttctcttccg cctttttctc ttccgccttt ttctccgcgg ctttcttaaa
     5821 tttttcgatg agcgccttct tttctttttc acgctcggat cgttcggcga tgtcacgctg
     5881 gcggtcaaaa tagacgcggc catcgatcag agttttctgt accacagcgt aagcgctcag
     5941 cgggtcgtga ttgtaaatcg ccaggtcggc gtctttccca acgtcaatcg tgccaacacg
     6001 cttgtcgatg ccgagctgca acgcgggatt cagcgtaacc agcttgagcg cttcgtcgtg
     6061 cgagaggccg ccgaacttca tgctcttggc ggcttcctga ttcaagtgcg tggcttcttc
     6121 cgcgtcgtcg gaattgatgg agacgaccac gccgcggcgg gtcatcagcg cggcgttgta
     6181 cggaatcgcc tcataggctt ccactttgta ggcccaccaa tcggaaaaag tggaagcgcc
     6241 aactcctgcg gcggccagtt catcggcgac tttgtaacct tccaggatgt gctgaaaggt
     6301 gcggaccttg aagccaaact ctttcgccac gcgcagaagc atcaggattt catcttcccg
     6361 gtagcagtgc gagtgaacgt agcgcttgcc ttccagcact tcgacgagcg gctcgaggcg
     6421 cagatcgcgg cgcggcgcga tgagattttt ttcaccaacg gcgaggcgct tgttgtagcc
     6481 ctcccacgct ttcttgtagt cgcgggcttc actaaacgcg ccgcgaatgg tctcttcgac
     6541 gcccatgcgc gtggaaggat accgccgggg ctgacccggg atactgaagt tcgagcgctt
     6601 gggattctcg ccgagtgcga acttgatgcc gggaagcgcg ccttcaaacg ggagcttcgc
     6661 ggccggctgt ccccagcgaa gcttgatgac cagcgtctgt ccgccgatgg agttggcgga
     6721 gccgtgaaga atgtttgctg acgtgactcc gccagcaaga tcacgataaa tatcgatgtc
     6781 gtccggattc agaatctccg ccatattcac catcgaggaa acggaaacgc tgccctcgtt
     6841 gatgctctcc gcggcgatgt gcgagtggca gtcgataatg ccgggcatga cgaattgtcc
     6901 cgcggcgtcg atgacctgcg cgtctctcgg ggcgttgatg gactggccga cttcggcgat
     6961 tttgccatcc ttaataagga tggagccgtg ctcgatggtt ccatgcgaga cggtgaggat
     7021 ggtggcgttt cgaatcacaa tgacggcggg cgcgtccgtc ggcgtggcag catgcgccgg
     7081 aaccgagaag tagatgatgc aaagggagca aaggaagtag aggaacgaag tccgcaggcg
     7141 gcctgcgttt agcacattcg aaattctcac tgcgcacctc ccaggctggc catcgtcgct
     7201 gaagccgcgc cgggtttgac gccggtaaag tccagcgaga aaccctgcac gctgatgctg
     7261 cccttcaagg aagttccatc aaacgtgcca gagaaagtga cgtcggcggg agatccttcg
     7321 agcggaatgt tgatggtgaa actgaatttg tccacgctca agtagccgct gatgagcgag
     7381 gtcgtgccgc gtttgctggt gacagtgccg gagattgtgc cgtccttctc catcgcaagg
     7441 tccgcggtag attcctcggg accatcgggc gtggtgtagg aaagcttcca ttttccggtg
     7501 aggtcgcctt tcggcggctc cttgggcttc tccggctcgc gaacctcaaa tctgcggccg
     7561 tcgacaaaaa ccattttgat cttcgttttt tcgtcgaaga ggtcgccgtc ggcgacgacg
     7621 aggctggcga tcttcccgat ttcaatcgag cccaggcggt cggcgacgcc aaagatttcc
     7681 gccgcagaga gcgtcagcgc gcgaagtgcg gcatccggcg cgaggcctgc gtcaatggat
     7741 ttcttcgcgg ctttcagcgt gtccttcggc gccgttatgc cgccggaata aaacgcaaat
     7801 ttgacgccgg ctttggccaa cgccaccggt gaagacggcg cgcggtcgcg gaattgcagc
     7861 gctcgaagcg acggcttgtc ctcgggatcg gcgtcttttt cagtttcggg ccacttgagg
     7921 ttcacgagca caggcagttt cttcgcagcg atttccggtg cgacctcata agccatctgg
     7981 ccgccgtaga tagcgccgtt gacatgccag cgatcgacca attcgagggc gcggcggagc
     8041 tgcacggaat tgttcgccgg aatcaaaacg agcgcgtgat cttccagcgc attggctagc
     8101 gcggcttcgg tgcggtcata acggggccgc gccacgctgc gaggattctt ctcatacgtg
     8161 gcctgtgccc tggtgctcca ttccgtatcc agccagacct gatgaacgta cgcgaggacg
     8221 cccatgatcg agttgggaaa cccgccgccg aatcctcccg aggtctggaa taccaccggg
     8281 atcgccaccg gcgatttcac caccagatct cccgcgcgtt cgcccgccag atccagtacc
     8341 gccgcctgcc ccgggaagat cccccctttt ggcgcggaaa caacggtggt gaatcccgcg
     8401 ttgcgccagg tttcgatgcg cttgtcgctc aggctgactt cgtccgccgc gctgcgccac
     8461 ggcgtggagg cgggcctgtc ctccggaccg cgcgcgccct cttgcgtgcg ccgcggaccg
     8521 ccttcgccac tcgcaggagg cgcgacggga atccccacat cggtaaagga atcaaagagc
     8581 cccgggtaaa cagtgaggcc cttcccgtcg atgacccacg cttcaagcgg aatggcgata
     8641 tcttttccca cagccgtgat gaggccgcga gagacgacga tagtggcgtt ctcgatgggc
     8701 ggcccggaaa cggggacgac tctggccccg cgaatggcga agtattgcgg ctcgccacct
     8761 tgcgccataa gagacggcgc agcacagaaa agagccaaaa gggccgtgaa acagaccgct
     8821 gacacacacc tgagaatcag gttggaacgc atgcaagact cctcgcgaag gcgttgaaag
     8881 gaaaccgacc cccggcaacc gtaaaggtag acggatgagg ctagtgggaa gtttcggaag
     8941 agtcaagaag aggggggact gcaaaaaaag aaaaaatcct ggaaacaaaa ggagagggtc
     9001 ctgcgtttct tctattgcat tcccataggg actgtagcat ataggtatgg gtaagccata
     9061 ggagctacag gagaaaacga tgggcgaaag agcggacgtt tggcaaggca cgctggcctt
     9121 gatggtgctc aagaccttgc aggcgctggg gccgctgcac gggtacggga tcgcgcgccg
     9181 catcgaacaa accagcggcg acctgctggc ggtgaactac gggacgctgt accccgccct
     9241 gctcaagctg gagcaggagg gctacatttc ctccgagtgg ggcacgtccg acaacaatcg
     9301 caaagccaag tattacaaac tgacgcgtgc cgggcggaag caattggata aagaagcgcg
     9361 cgagtgggca cagacgacgg agatcttggc gcggttcctg gctcccggaa aggaatccgc
     9421 atgaggcggc tgcgcgcgct gttcatccgg ctgggcgggc tctttcgcga agaccggcag
     9481 gagcgggagt ttaccgctga gatggaaagc cacctccaaa tgcacatcga agacaatttg
     9541 cgcgcgggca tgagcaccgc ggaggcacgt aggcaggcgc tgatcaagct cggcggcatg
     9601 gaacaaacca aggaaaatta tcgcgaccgg cgcggtcttc cgcttcttga aacgctgttc
     9661 caggacttgc ggtacgccgc tcggatgctg ctgaaaaatc ccggtttcac ggccgtcgcg
     9721 gtcattacgc ttgctctcgg catcggtgcg aacaccgcga ttttcaacgt ggtgcatgca
     9781 acccttcttc agccattgcc gattcgacat gccagccgcc tggtggtgat ctgggtgaac
     9841 aacctcgaac acggttggag tcggatcggg ccgacagggc aggactacct ggattggaag
     9901 gagcaggcta agtctcttga cgacatcttt ttgttcgagc acggcaccgg aaccgtgacc
     9961 ggaccaggcg agcccgaaca agtggcgggc ttgcgagtaa cgacgaattt cggcggcttt
    10021 tttggaatca agcccgtctt tggccggacg tttcgtttgg aagaggcagg gggccgccac
    10081 aacttcgtaa tcctaggcaa cagctactgg cgacggcgct tcagttccga tccatccgtg
    10141 gttgggcgcg gcatgacgtt gaacggcgag gagtacacga tcatcggagt cttgcccgcg
    10201 gaattcgccg ccctttttcc cgcggatgta gtcgtgccct tcgacaccgc ctgggtgaag
    10261 cgtgttgata ccgaccttgg cgttttcggc aggctcaaac ccgaagccac actaaaccag
    10321 gctacccggg agatgagcgt catcgcggaa cggatcgcca tcgcgcgtcc gtcacggaaa
    10381 ggctttggcg tagttttggt gccactcgaa agcgtacgct tggagtatct ccgtcccgcg
    10441 ctgctcgtct tgcttggtgc cgttggtttt gtgctgctca tttcttgtgc caacgtcgcc
    10501 aacctcttgc tggcgcgctc cgttatgcgc cagcgggaaa tggcgattcg gatggccctg
    10561 ggagccggac acctgaggct gatccgtcag ttcctcgctg agagcacgtt gctctcactc
    10621 ctcggcggcg ctgtgggttc gcttctggcg ctatggtcca ctgagttgct gaaaatcttc
    10681 ttgccttcgc gtattcccgt tccgaatgca gccgacgcgg tcatgttgcc gagaattcac
    10741 atgagtggcg ccgcgttcgc tttcacgata gtcatatcgc tgttgacagg aattatcttc
    10801 ggcctcattc ctgcccttca aagcctgaag tgcaacgtca acgaatcgct gaaagagggt
    10861 gggagaggct tcctatctgg cccgcgcgga catcgtaccc gctccgcact tgtcattgcc
    10921 gaatctgcct tggcgtttgt gctggtgatc ggcgccggcc tcatgatcaa gagcttttgg
    10981 cgcctgttgg aggcaaaccc cggctttcat cccgatcatc tgctgacctt acggattaaa
    11041 ttgcccgcgg atgcgaagga ctccaaatat cgggagcctc gccagcaggc cgctaccttt
    11101 cagcgcttcc tggccagcgt tgaggcagtg cccggaattc agtcggccgc gttcgcggaa
    11161 attgttccgc tcagccagga cgacatggac atgggatatt tcaccctcaa ggaaggtccg
    11221 cttcggccgc cgggcgagca tctcgcagcg gactacaggg acgttacttc taattacttt
    11281 gccgccatgg gcattccgct gattgcgggc cgaatcttca cggaacagga caacttagac
    11341 cgcccgcggg tggttgtgat tgacgagccg ctggcccggc gatttttcac cggcgaggac
    11401 ccgataggca agcatttaca aattcccgat tacacgcggc ccgcacgcga aatcgtcggc
    11461 gtcgtcggcg gagttcgcga cactgggttc gatcagccgc cgcgccccac gatctacttt
    11521 ccctctttgc agagccccga ccagacaatg agcctggtgg tgcgcacgac cttgccgccc
    11581 agtgcgatct tgcccgcgat caaaaatgcg atttggtcgg tcgacaagaa ccagcctgtt
    11641 ttcgaagtca ggtcgatgga cgaaatcata tcgggaatcg tttccgcgca acacctcgct
    11701 tttctcttgc tgggagtctt tgccttcctg gcgctcgcgt tggcagcgat tgggatttat
    11761 ggggtgactt cctacgtggt cagtgaacgg acgcatgaaa tcggtgtccg catggcgctg
    11821 ggcgcccaac cgcgcgatgt ttcgcgcgtt gtgctcggcc ttggagcaag gctcgctggc
    11881 atcggggtca tcggcggcgt actggcagct ctcgcactca cgcgcttgct ctcaagtttg
    11941 ctcttcggcg tcagcgcgac cgatccgttc acatttgtcg gcgtggcgat tctgctgacc
    12001 cttgtcgcgc tcgcagcctg ctacatcccg gcgcgacggg cgatgcgcgt cgatcccata
    12061 gtggcgctgc gctacgaatg agcttcaaag gcctgaagca ggaagttagc gattgaagtc
    12121 gagagtggca gagggcgcta tgaaatggtt gcgtggattg ctgttgcggc tgggagaact
    12181 cttcggtaaa gagcggcgag agcgcgaact cgccgctgaa cttgaaagtc atctccaaat
    12241 gcatatcgaa gacaatctgc gtgcgcgcat gagcaccgcg gaggcacgta ggcaggcgct
    12301 gatcaagctc ggtggcatgg aacaaaccaa ggaaaattat cgcgaccggc gcggccttcc
    12361 atttctcgaa acgctgttcc aggacttgcg gtacgccgct cggatgctgc tgaaaaatcc
    12421 cggtttcacg gtggtggcgg tgttgacgct ggcgataggc atcggcgcca acaccgcgat
    12481 cttcagcctt gtaaacggag ttctcttacg cccgctgccc tatcgtgaac ccgcgcggct
    12541 gacgattgtc tggggaaagg acgatcaagg aaaaccaggc aacgtgggtt atgcgaccta
    12601 tgtggactgg aaggcgcaaa acaagagttt tgaagaattg gctctgtatc gctcctggtc
    12661 gcccgtgctg cagaccaacg aaccggaaca gttgagcgga ctccgtgtga caaacaatta
    12721 tttccgcgcc cttggcgtcc atccgcagtt cggccgggat tttcggccgg aagaggacat
    12781 ccccgccgca agtcatgtgg taattctcag ccacggcctc tggcagcggc ggttcaactc
    12841 ggatcagagc atcatcggca gagctattag cctgaatgcg acaacttacc ttgtcgctgg
    12901 cgtactgcca gccgattttc aatcgttaat ctcgatggat ccgcacggcg gccccgtgga
    12961 catctgggga gtgctgggat acgacgcgtc actgccgtgg gcctgccgta cttgccagca
    13021 tctggtcgcg attgggcgtc tccggccggg cgtggcgttt acgcaggcaa ctgcggagat
    13081 ggacacagtt tcctccgcgc tttggaaagc ctacccaaag gagtattccg cagccggtgt
    13141 aattctcacg ccgctccgcg agcatctggt cggtcccgta agcaccacgc tctacattct
    13201 cctcggtgcg gtgtcgtttg tgctgctgat tgcctgcgcg aaccttgcaa acctgctctt
    13261 ggcacgcgca actcatcgcg aaagggagat ggcggtgcgc acggcgctgg gtgccgcgcg
    13321 tggcagaatc gtgcggcagt tgctcgtcga gaattgcttg cttgccgcat tgggcgcggc
    13381 ggccggcttg ctcctcgcgt attggacgcc ccaactgttg cgcgttttgg gggcaggcga
    13441 attgccgcgc ctggatgaag tgaaggtcga ttcacacgta ttgttgttta cgcttggcat
    13501 ggcattactg acaggaatcc tttccggttt ggcgccggca cttcgtttgt cgaaaaccaa
    13561 cttgcacgat tcgctgaaag aaggcgcgcg tggaacatcc gctggcacag gccggcgcat
    13621 gcgcagcttg ctcgtggttt cggagattgc gctttcactg acgctcctgg tcggagcggg
    13681 attgctgttg cgcagcctgt cgcaggtgct tggcgtttcg gcgggctttg attcaaatca
    13741 cgttttaacg atgagaatct ccgttctagg ccagcggtat aacgacgaga agaacctgcg
    13801 gcagtttttt acgagtgcgc tggcaagtat tcgcgcgttg cccggagtgc aatcggccgc
    13861 tgtaaccagc caagtcccgc tcggcggcaa tggggatcgt tacggtttcc acgtcgaagg
    13921 gaagattcat gtgaacccgg agcttgacga aagtgcggag cgcttctgcg tttctccggg
    13981 gtatctcgac gcgatgcgca tccggctctt gcgcggaaga gatatttctg aaagcgatag
    14041 ctcgaccgcg cagcagactc tattgatcag tgaaactacg gcccgcagca tgtggcccgg
    14101 agaagatcct atcggcaagc gtgtaaagct cggcggggtc gatcttccct ggtggacggt
    14161 ggccggagtt gtggcggacg ttcatcatga tggcctggac atcgctccca ccatgcagtt
    14221 ctacgtcccg catgcgcaat ggccataccc cgattccgac atgactttca caattcgtac
    14281 cgcggggccg ccgggcacta tggcgtccgc tgcgcagcaa gcaattcacg ctcttgaccc
    14341 cacgcagccg ctttcgcgcg tcatgccgct cgcggactac gtcgggcttt ccgtgcaggg
    14401 caggcggttc tcctccatct tgctcgcggc gttcgcagcc atcgcgctct tgctctctgc
    14461 tctcggaatc tacggagtca cggcgtacag catcgcgcag agaacacgcg agatcggcat
    14521 ccgcatggct cttggggcgc aacagaagga agtttttgcg cttctgctgc ggcaatcggt
    14581 ggtcctggtc gttttcggtg tcgccctggg tgtcgctacc tcggccgcgc tcacgcgatt
    14641 cctcgcaagc atgctgttcg acgtgagacc caccgatccg gcaacatttg tgagtgtggt
    14701 gtctctcttg gtgggaattg cggcgctggc gtgctggttc ccggcacggc gcgcgatgcg
    14761 cgtcgatccg atggtggcgt tacgttatga gtgaagaaca cttttcagtt ttcagttctt
    14821 agtttttagt tcttagtttt cggttcttag ctcttgcttc tatccttcga tgagatcttt
    14881 cgcttcgctc aggatgacaa tcgcggcgaa attcgccttg aaaatataca cacttatgac
    14941 gagaaatccg agctagggct ttttcttttt gaagaggccg ccgagagcgt ttatggcgtc
    15001 ggcgggattt tgcccctgcc ctggcacttt ggcgatgctg gcagtagagc ggccaccgag
    15061 gcaacccaac tcagacttga aaagatcttt ggcaagacct ccgatgtccg gaatgaattt
    15121 gggatcggag gtggtgccct gaatctgaaa aggaactttc agtccgccgc tgctgctggt
    15181 gcagccgccg acgccgccag cgccaccgcc gccggtaacc gtaccgagga gtccaccgag
    15241 agcgcccgct gcgccatttg ccacggcgcc tgctgagcct gccgcgctgc tcgcctgttt
    15301 cgtgagcgtg gcgagcattt tgaaatcgag agcgttcttc gagtccacgg tgcccgcgcc
    15361 ggtgagattc ccaatggtcg gaaggaccgc aagaacgttg tccgctttga tgccgtcggg
    15421 ggccacgcgc agattcgagg tgaacttctc gatgttgaga tccttgcccg acttgatgcc
    15481 cgcgagagaa gcaacagagg acattttgga gccgagatcg aagttcgcga tgacgccgtt
    15541 gaagagaccg acggtgccgg tggtgaccat tttactcatg gggccggcca gggtgagatc
    15601 ggtattgagc gtgccagccg agagagaagc gcccttggga agatggatgc cgagcgcggg
    15661 aaggaaagct tcgagatcct tggcgggcat gtccttggcg ctgagcttga tattggcgac
    15721 ggtggcctcg cccgcggact gataggtacc gttgaggcgc gcggccgcgc tgccaatctt
    15781 cagcgtggaa ggattcaaga cgccggcgtt cttgcggaga ttgtatttgg tattgaaatc
    15841 cacgatgacg gggacaccgg cgggtgctcc gccggcgacc aagagtgcat tggaaagttt
    15901 gacgttgccc ttgatttcgg cctcgccgcc tttcgattcc agcgtgccgt cgagatcgag
    15961 aaggcccccg agtcccatgg agggatcgag gaaacccgtg gaggcgagat tcaaagagct
    16021 gacattcagc ttggcggtga gcggagtcag agcggtgtcg gcttgatcga tggggccgac
    16081 gatgccgtcg agcttgaact tgccgccgga aggaagatcg gcggtgacgg taaccggaaa
    16141 ctgcgaggtt atggcaacgc cggtggctgt gacggctacg tgatcatacg tgctgcgctt
    16201 ctgcgaattc gtcgagccaa cgatgatgct gccgtctttg agctcgagct tcttgatggt
    16261 gagatcagct ggagcattcg tcgaccccgt ggatttcgcg gcggagccgc ccagcgaaga
    16321 gtagtt
//
LOCUS       DASYVO010000478         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_220292, whole genome shotgun sequence.
ACCESSION   DASYVO010000478 DASYVO010000000
VERSION     DASYVO010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220292"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VGR03_16405"
     CDS             complement(<1..1150)
                     /locus_tag="VGR03_16405"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1995916.1"
                     /translation="MNLRRVVLFVFAAVLVLAAVRAGSARPAGDEASAIAAADAQILS
                     EVREHSEAMQNSEYLGDAIGPRLTGSPQLTQANEWTAAKFREYGLTNVKLEPWTIAHS
                     WMRGTAHARIVSPTEHPLTIASAGWAPSTAGVVRGPVVYVDIKKDEDFEKYRGKLKGA
                     IVIAQEPVSLSPPKPDGFFGEGNRPMQTPPPLKGQPVPPSPFAAMMAVARKRTEFYKQ
                     EGVAVVLRDSNKPHALLNMTGIGGEKFEIGAIPTAFITGEGYRMLWRMQKHGPVQVEI
                     EMTNSFSDKPVEVYNTVAEIKGSEKPEEEVILGAHLDSWDLGTGSTDNGTGSMAVLEA
                     ARALAKLNLKPKRTIRFILFSGEEEGLCGSVKYVEAHKAELEKISGVLV"
     gene            complement(1343..2254)
                     /gene="mutM"
                     /locus_tag="VGR03_16410"
     CDS             complement(1343..2254)
                     /gene="mutM"
                     /locus_tag="VGR03_16410"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEV1995917.1"
                     /translation="MPELPEVETVARGLRQAILGRRILSVTLGKTDFIDDPAALEQHL
                     PGRRIEAVERYGKFMLLRLSALDGETRVATNGDAAPASLLVHLGMTGQIAPTPAGQPL
                     EKHTHVCLLLDDGRELRYTDARRFGRIAYLTKALLAEELTGFGADPLEVSKEEFADRI
                     CGRRARIKALLLDQSVLRGVGNIYADESLWRARLHPARLGAKLTKKQAETLRRVLQDI
                     LQKAIVLRGSSISDFLDAEGEPGEYQRHHRAYGREGKNCYRCKTPIRRAIVAGRSSYF
                     CPKCQRSPRGFAMLPLPWRGKGRTRKR"
     gene            complement(2449..>3233)
                     /locus_tag="VGR03_16415"
     CDS             complement(2449..>3233)
                     /locus_tag="VGR03_16415"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEV1995918.1"
                     /translation="SSVFVICLAALSTLFLQNTHHDSMLEGVPSGKTRVLDLSYAISD
                     KLVPWPGDEKFFEAKVNAKIEKNGYFTRSFWMLEHYGTHLDAPAHFPPGKTTVDQIPV
                     KQLFGPAVVIDVRAESGMDADYQLAAARVEEWEKRHGRIAEGAIVLLRTGWASRWPDA
                     QKYRNQDAKGKMHFPGFSTEAAKLLLGRKVSGLGCDTLSIDYGASEDFAVHHLSLGAG
                     LYHLENLADLSELPETGAFLIVAPIKLEGGSGGAVRVFALLP"
BASE COUNT          643 a         1078 c          821 g          691 t
ORIGIN      
        1 gtaccagcac gccggaaatc ttctccaact cggccttgtg cgcctccacg tatttcaccg
       61 agccgcacaa tccctcttct tctccgctga acaagatgaa acggatggtc ctcttcggtt
      121 tcaggttcag ctttgccagg gcacgcgccg cttccagtac cgccatcgaa cccgtaccgt
      181 tgtctgtcga gccggttccc aagtcccagg aatccagatg cgctccgagg atcacttcct
      241 cctccggctt ctcgcttcct ttgatctccg cgacggtgtt gtaaacctcc acgggcttgt
      301 cgctaaacga attcgtcatt tcaatctcga cttgcaccgg tccgtgcttc tgcatccgcc
      361 acagcatccg gtagccttcg ccggtgataa acgccgtcgg aatcgcgccg atctcgaatt
      421 tctccccgcc gatcccggtc atattcagca gggcgtgcgg cttgttcgaa tcgcgcagca
      481 ccaccgccac gccctcttgt ttgtaaaact ccgtacgctt ccgcgccacg gccatcatcg
      541 cagcaaaagg agaaggcgga accggctggc cctttagcgg aggcggcgtc tgcatcggac
      601 gatttccttc accgaagaat ccgtcgggct ttggcggcga gaggcttacg ggctcttgcg
      661 cgatcacgat cgcgcctttc agcttcccgc gatacttctc aaaatcctca tctttcttga
      721 tgtcgacata caccaccggt ccgcgcacca ctcccgctgt gcttggagcc catcccgccg
      781 acgcaatcgt caaaggatgc tcggtggggc tcacgatccg cgcgtgcgcc gttccccgca
      841 tccaggaatg tgcgatcgtc catggctcca gcttcacgtt ggtcaggcca tattcacgga
      901 actttgccgc cgtccactcg ttggcttgcg tcagctgtgg cgagcccgtc agccgcggcc
      961 cgatagcgtc acccagatac tcgctgttct gcatcgcctc gctgtgctcg cgaacctcac
     1021 tcagaatctg cgcatccgcc gcggcaatgg cgctcgcttc atctcccgct ggccgcgccg
     1081 accctgcgcg gactgcggcc aatacgagta ccgccgcaaa cacaaaaaga actactcgcc
     1141 gtaagttcat tcgctctccc gtgccgtatt cggccaaatt ttcggaaaga atttacttat
     1201 cctcgcgcag ccaatccccc catgacaccg tcatgactgt gcgccctaat ctggaacttc
     1261 tctctataga cgtgctgacg cctgtcctgc gtttcgtcgg atgtccttct ctgcgctcct
     1321 cagcgcgctt tgcgtctctg ggttatcttt ttcttgttct tccttttccg cgccacggca
     1381 acggcaacat cgcgaacccg cgcggcgaac gctggcattt cggacaaaaa tagctacttc
     1441 gccccgcaac aattgcgcgc cggatgggcg tcttgcaacg ataacagttt tttccctcgc
     1501 gtccgtacgc gcgatggtgg cgctgatatt ctcccggctc gccttcggcg tcaaggaaat
     1561 cggaaatcga ggacccgcgc aacacgatcg ctttctgcaa aatgtcttgc agcacgcggc
     1621 gcagcgtctc cgcttgtttc ttggtcagct tggctcccag ccgcgctgga tggagcctgg
     1681 cccgccatag gctctcatcc gcatagatat tcccgacgcc gcgcagcacg ctctggtcca
     1741 gcagaagggc cttgatgcgt gcgcgccgtc cgcaaatcct gtctgcaaat tcctctttgc
     1801 tcacttcgag cggatcggcg ccgaatcccg tcagctcttc ggcaagcaac gctttcgtca
     1861 ggtaagcgat gcggccaaaa cgccgcgcat ccgtgtagcg cagttcgcgc ccgtcatcca
     1921 gaagcaggca aacgtgagtg tgtttttcca gaggctgccc cgctggagta ggcgcgatct
     1981 ggccggtcat tcccagatga acgagcagtg acgcgggcgc cgcatcccca tttgtggcaa
     2041 cgcgggtttc tccatccagc gccgacagcc gcaacagcat gaatttaccg taacgctcca
     2101 ccgcctcgat gcgacggccc ggcaaatgct gctcaagggc cgccggatca tcgataaaat
     2161 cagtcttacc cagggtgaca gataggatcc gccgcccgag aatcgcttgc cgcaatcctc
     2221 gcgctacagt ctcgacttcg ggaagttccg gcataagaag agagcatcat caaaatatca
     2281 cagtgccgcg ctcgatgccc ttcagaggat tgagcgagcg cgtctggctc aaggcgaact
     2341 gttccggcgg atgctttcgc aaactctggc tggaaaacaa ttgagctatc aagaaagcag
     2401 tcgaggcgta agaaaaaggg gaagaggttg gcaggaaatc aagcttcttc acggcagaag
     2461 cgcgaacacg cgcaccgctc cacccgagcc gccttccagc ttgatcggcg ccacgatcaa
     2521 aaacgcgccc gtttccggca attcgctcaa gtcggccaga ttttcgaggt ggtacagacc
     2581 cgctcccaat gacagatgat gcaccgcgaa atcctccgac gcaccgtaat cgatgctcag
     2641 cgtatcgcac ccaagcccgc tgaccttccg cccaaggagc aacttcgcgg cctccgtcga
     2701 gaaccctgga aaatgcatct tgcccttggc atcctgattc cggtatttct gagcatccgg
     2761 ccaccgcgac gcccaccccg tcctcagcag cacaatcgcg ccttcggcaa tccgcccatg
     2821 ccgcttctcc cattcttcga cgcgtgcggc ggcaagctga taatccgcat ccatgccgct
     2881 ctccgcgcgc acatcgatca ccacggcggg cccaaataat tgcttcacgg gaatttgatc
     2941 aacggtcgtt ttccccgggg gaaagtgcgc tggcgcatcc agatgcgtcc cgtagtgctc
     3001 cagcatccag aagctccgcg taaaataacc gttcttctcg atcttggcat tcaccttcgc
     3061 ttcgaagaac ttttcgtctc ccggccacgg caccagcttg tcgctaattg cataactcag
     3121 gtcaagcact cgcgtcttcc cggagggaac accttcgagc atcgaatcat ggtgagtgtt
     3181 ctgtaagaag agcgtggaaa gggcagccaa acaaataaca aaaacactgg agc
//
LOCUS       DASYVO010000479         3204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_181790, whole genome shotgun sequence.
ACCESSION   DASYVO010000479 DASYVO010000000
VERSION     DASYVO010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181790"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VGR03_16420"
     CDS             complement(<1..589)
                     /locus_tag="VGR03_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995919.1"
                     /translation="MIQEQRGGARAFVRSLNILLKFARLYEFGHVRTIQQFETAWQEL
                     RNALDDSGGSGLLLGASGTQILLDGVPLGSAAGERSFAQMLISSGIASIHFSPTLTQP
                     QFARFVRAFPSGNAKPSSLAEQLKSALAGETSIKVNEIRYVAEDSSIAGIKVAAQLTQ
                     KVLGAQGDKFKDFFEDPNKLLQMILAAESSRAGGGG"
     gene            1512..2159
                     /locus_tag="VGR03_16425"
     CDS             1512..2159
                     /locus_tag="VGR03_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995920.1"
                     /translation="MSFRKGSPRHVLAGRVFVASMLTMAVFAVYLAITRHQPSNIGGG
                     FLTVYLIGTAWLTARRRDGETSRFDWVAVLIPLALGILNWMNGLKGVRSGASSQGGVP
                     VGMIFFMGSVCLLAAAGDVRMLVRGGVFGAKRIARHLWRMCFGLFIAAGSFFLGPSNR
                     PLRLLSTVGLGQHLSSALFSTGLYLVLTILPLILLIFWLVRVRFTNAYRKIETRN"
     gene            2268..2519
                     /locus_tag="VGR03_16430"
     CDS             2268..2519
                     /locus_tag="VGR03_16430"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEV1995921.1"
                     /translation="MALKTRMRVNENGRVVIPASFRKRLGIRVGDEVVLQIQDDELRI
                     TTLKRNLERAQRLVRKHVKAGTSLVDELIAERREAARNE"
     gene            2512..2898
                     /locus_tag="VGR03_16435"
     CDS             2512..2898
                     /locus_tag="VGR03_16435"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEV1995922.1"
                     /translation="MNRVVLDASALLAILNQEAGAETLTPELLSGAAMSTVNLAEVHG
                     KLVGRGLRPDDAWDAALSAIREAVPFTSEHARLVGDLVAQTRPLGLSLGDRACLALGL
                     SLKAPVYTADKSWKKLKVGARIHVIR"
BASE COUNT          601 a          947 c          922 g          734 t
ORIGIN      
        1 cgccgccacc gcctgcgcgc gagctttccg ccgccaggat catttgcagc agcttgttgg
       61 gatcttcgaa gaaatctttg aacttgtcgc cctgcgcgcc gagcaccttc tgcgtaagtt
      121 gcgccgccac cttgatgccc gcgatggagg aatcttccgc cacgtagcga atctcattca
      181 ccttgatgct ggtttccccc gccagcgcgg acttcaattg ttccgccagc gaagacggtt
      241 tggcgtttcc gctcgggaag gcgcgcacaa atcgcgcgaa ttgcggctgc gtgagcgtcg
      301 gggaaaaatg gatgctcgcg attcccgagg aaatcaacat ctgcgcgaag cttcgttcgc
      361 ccgcggccga accaagtggc acgccgtcca gcaaaatctg cgttcccgac gcgcccaaca
      421 gcagaccgct gccgccgctg tcatccagcg cgttgcgcag ctcttgccat gccgtttcaa
      481 actgctgtat cgtccgcacg tgcccaaatt cgtacagccg cgcgaacttc agcagaatgt
      541 ttaggctgcg gacaaacgcc cgcgccccgc ctcgttgttc ctgaatcata gctctcggcg
      601 caggactctg cccctcctca cgcttccctc tacagtagat tcacccgcgc cccgagacaa
      661 gcctccccgc gcctcttgta accatctcaa taacagattc ccgtatcgtt ttggaaacat
      721 tgttgccccg cccgttcctc ttcagtcttt gcctctgtag gggcacgagg aacttgtccc
      781 gacccggtcg ggatcgtgcc ctctcttcgc tgtcggtttt gtaggggcgc agcacctgtc
      841 ccgaccctgt cgggatgctg cgcccttctt cggcccgagt aaggccgggc tttcccaggg
      901 gtcccggcga ttggcgttta cccccaccaa cgcgtcgggg tcggaaagcc cggcatctcg
      961 gttctcttgg gtttctctgt tgtagggctg cccttccgag ggcggcccct ctttactgtt
     1021 gttgtggcgg gtcttccgcg gttcttgccg tccggctcct gtcggaccga cccgctcttc
     1081 ccacttcccc gcgcaggaga tcggctgctt accatcgctg tcgttcgtct ttggaatttg
     1141 tcgtggcact ttacacttta gggcgccatc ttgttcttgt agcggcggcc tctttatcct
     1201 cagcctgccg aagggcccag gccggcatct cctggaaggt agcggccgcc gcccgcgcta
     1261 ccgtcgcggc cctcgggact ctcgccggcc ttcttaccca cactctcagc taggggtgcg
     1321 cgcttcatag aagaaaatta ggagcaagat ccgcaccctg aaaaccggcg ttcagtgctg
     1381 atagcggtgg ggtgaaacac cggggcaccg gtccacgtat atatctccaa gaagggccaa
     1441 tgtcctattc gcccatactg attgttcata ttttcggtgg gtcctcgggg ttgctgtccg
     1501 ggaccgctgc aatgtctttc cgcaaaggtt ccccccgcca tgtgctggcg ggaagggtct
     1561 ttgtcgcctc catgctcacc atggccgtct tcgcggtgta ccttgccatc acgagacatc
     1621 aaccaagtaa catcggcggc gggtttctaa ccgtttatct gatcggaacg gcgtggctaa
     1681 ccgccaggcg cagagacggg gaaacgagcc gttttgattg ggttgcggta ctgattccct
     1741 tggcgctcgg catcctcaac tggatgaatg gtcttaaagg cgtgcggagc ggagcgagtt
     1801 cgcaaggcgg agttcctgtc ggaatgatct ttttcatggg ttccgtgtgt ctgcttgctg
     1861 ccgcggggga cgttcgcatg ctggtgcgcg gcggtgtttt tggagccaag cgcatcgcgc
     1921 ggcatctgtg gcgcatgtgc tttgggctgt tcatcgcagc agggtctttt ttcctgggac
     1981 cctcaaaccg gccgctgaga ttgctttcga cagtagggct cgggcaacat ctatcctcgg
     2041 ctttattcag cacaggcttg tatttggttc tcactatact gccactgatt ttgctgattt
     2101 tctggctagt gcgagtccgc ttcacaaatg catacaggaa aatagaaact agaaattaga
     2161 aaagggacag gagaaagacc acaccgttca cccgcaactc cgaaaaggtg tcacttctaa
     2221 tctggaatga catatgctac aatagtgtca caggaaagag gcatgacatg gcactaaaaa
     2281 cacggatgcg ggtgaacgag aacgggcggg tggtgattcc tgcttccttt cgcaagcggc
     2341 tgggcatccg ggttggagac gaggtcgtcc tgcagattca ggacgatgaa ttacgcatca
     2401 ccactctgaa acgcaacctt gagcgggctc aacggctggt ccgcaagcac gtgaaggcgg
     2461 gcacctctct ggtcgacgaa ttgattgccg aacgccgcga ggccgcgcga aatgaatagg
     2521 gttgtactgg acgcttcggc cctgctcgcc attctcaatc aggaggccgg ggccgaaacg
     2581 ttgacgccgg agcttttgag cggcgcggcc atgagcaccg ttaatctggc cgaagtgcac
     2641 ggcaaacttg tcggtcgggg cctgcgcccg gacgatgcgt gggatgccgc gctgagcgcc
     2701 atccgggagg ccgttccgtt tacctcggag cacgccaggc ttgtgggcga tctggtcgcg
     2761 caaacccgcc cgcttgggtt gtccttgggt gacagggctt gtttggcgct cgggctttcc
     2821 ttgaaggcgc cggtatatac ggcggacaag tcctggaaga agctgaaggt cggcgcgcgc
     2881 atccacgtca ttcgctgaca gtccgctcaa ctagaaaata gaaagtagaa attagaaaaa
     2941 aagagaaaga ccccaccgtt catcccgcaa gagcagcggg atggagcgga gatgcaaaac
     3001 tctcaaggat tgggccaccc aaaggctttt ttcggaattg aggtgcggcc actcgccttc
     3061 acaaagcctt ggggtactcg aagtacgtgc caattgcttc gcggtaatcg tactggaggt
     3121 catggttggt cttgtcgaga aattccttga agtcgtcaag gaatctcacc catcgctgct
     3181 ggaactgttt catcctttcc tgat
//
LOCUS       DASYVO010000480        11170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_82386, whole genome shotgun sequence.
ACCESSION   DASYVO010000480 DASYVO010000000
VERSION     DASYVO010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 11170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11170
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82386"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            435..1574
                     /locus_tag="VGR03_16440"
     CDS             435..1574
                     /locus_tag="VGR03_16440"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEV1995923.1"
                     /translation="MGFATNAIHVGQEPDPATGAIVAPIYQTSTYINEELGKNKGYDY
                     ARTNHPNRKALERTVAKLEGGHSAYVFSSGMAGIDAVFRLLRPGDHVVLSEAVYGGVF
                     RLSTQLLVHFGLEFSFVDTSVPDFVRNALRPNTKMLYIETPANPTLSVTDIAAISKLA
                     NERNLSVVVDNTFLSPYLQRPIELGAHIVVHSMTKYLNGHSDSTGGAVVLTRPEDAEK
                     IYFVQRSAGTGLAPMDCFLISRGIKTLAVRMLQHNANGITVARHLDAHPKIRKVFYPG
                     LPSHPQHEVARRQQKGPGAMLSFDVGSLEAARRFLNHVKLCSLAESLGGVETLISLPA
                     MMTHASMPKEVQDRVGITEGLVRLSVGIEDVEDIIADLDQALLYV"
     gene            2144..2866
                     /locus_tag="VGR03_16445"
     CDS             2144..2866
                     /locus_tag="VGR03_16445"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEV1995924.1"
                     /translation="MATLTAPRIESANAKLVAPPWHTVVLVTLFLILALGGAFFQRET
                     RTHPGILQQHPHVAPLYFYLIAMEWGLFVYVWKGGLRRTGTKLSELIGGRWTSAREVL
                     VDAALALGLWGIWTLLEMIWVRRLGPDHAASIQTLLPRHALEILLWIAVSISAGICEE
                     FVFRGYFQKQFEAFTHRKWIALFLQSVLFGISHGYQGIEACVKIAIFGSLYGLLALWR
                     KSLRPGMMAHAWSDILSGIFGI"
     gene            2941..3414
                     /locus_tag="VGR03_16450"
     CDS             2941..3414
                     /locus_tag="VGR03_16450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV1995925.1"
                     /translation="MTGFGALYKKYAPDVYHFALYLSGERGEAEDITSETFVRAWASS
                     EPIRMATVKGYLFTIARNLFLQGLRKKSRHVVLDEDLRDPQASPYAQAEQKAELRAVL
                     AGLQKLPEIDRAALLMRAFDEVPYEEIARALGISLAAVKVKIHRARLVLADIRNA"
     gene            3454..3879
                     /locus_tag="VGR03_16455"
     CDS             3454..3879
                     /locus_tag="VGR03_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995926.1"
                     /translation="MNVTREVILDLLPVYLSGEASPATRALVEEYMKQDSELAQRIRL
                     QWAENFAKVAPSALPPDLELRSLLRTRRLIGLQRWLFGFGIFFTALSLSNEFSFAGGH
                     LKEFHFLLRDYPVEFGICLTLGLACWTVYFSIRRHLRTS"
     gene            4019..4150
                     /locus_tag="VGR03_16460"
     CDS             4019..4150
                     /locus_tag="VGR03_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995927.1"
                     /translation="MVSVDFTILGGFTALLLLVALGAGYLPARRAILVDPKVSLRYE"
     gene            complement(4166..4516)
                     /gene="cutA"
                     /locus_tag="VGR03_16465"
     CDS             complement(4166..4516)
                     /gene="cutA"
                     /locus_tag="VGR03_16465"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEV1995928.1"
                     /translation="MKVAGKYRAVLVTCGTLMEARRISRSVVGKRLAACVNIILSPAE
                     SFYTWKGELEKAREYLLVMKTSAKRLAELEKEVKRLHSYDVPEFVALPITEGSTKYLS
                     WLEDSVTKIRKKPD"
     gene            4636..6060
                     /locus_tag="VGR03_16470"
     CDS             4636..6060
                     /locus_tag="VGR03_16470"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HEV1995929.1"
                     /translation="MTTSPIKFGTSGWRGLIADDFTFANVRLAVTAIAVHVKTKARQP
                     TILIGYDTRFYSEEFSQLAVDILQHHGIRTLLCETFTPTPAIAFEIMRRKLDGAINFT
                     ASHNPAQYHGLKFSSADAGPALPEVTKDIEARAAQLAAKGGVPALPHGASKSARDEKV
                     NLRENYLKRLEELVHFETLRKAKPSLVVDALHGCGAGYLDRILTDHGVTVQAMRTERD
                     CLFDGTGPDVSEENLAPLRKAVRDSNATAGLATDGDADRFGIVDRDATWIQPNLILAL
                     VYDYLVETRKWKMPAARSVATTQMIDAVAKSHGQTVHQTPVGFKYIGQLIREDKIALG
                     GEESAGLTIRGHIPEKDGILACLLVAEMIAARGASIGEQVRAMYKKIGREFWPTRENL
                     HLSDEQKANAVRRVAVDASTLAGRKVVSIDRTDGAKFVFDDGSWMLLRLSGTEPLLRL
                     YVEAESAAASAKLTREASQWVLQT"
     gene            6042..6404
                     /locus_tag="VGR03_16475"
     CDS             6042..6404
                     /locus_tag="VGR03_16475"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV1995930.1"
                     /translation="MGTANLNKMGANEKIQPLLRHYGPALLGLLVLVLVVHDIFGTHG
                     YLAMRRTQQEIKKVTADLDQLNKENLQLEQEVRELKTDPHKIEKIARDELGLARPGEV
                     IIKIPRSQQLPQDPTSKP"
     gene            complement(6388..7500)
                     /locus_tag="VGR03_16480"
     CDS             complement(6388..7500)
                     /locus_tag="VGR03_16480"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEV1995931.1"
                     /translation="MADQIQSPARRLAYIDWMRGLACVLMFQTHCYDSWMSPEAKKSS
                     SLIAWSRLGGTLPAPLFIFLAGVSFALVTEKLREKGIKRNAIAKQTILRGAEVFGMGL
                     LFRVQEFALGYPWSPWTDLLRVDVLNILGLSMMLMGVLCWAAGDGAVAVARFRTQVAG
                     IFVAVAVAMATPPLWTTHRLNFLPWPIESYINGVYIFKEPQPWLFPLFPWSAFAFAGL
                     AVGFLLFSDFAKRMEGLTFAALGGTGIVACYSSVLLDSLRVRLYAVYDYWHSGPNFLL
                     MRCGILLIILFLVYAWCRWGFAQKGFSPIIQLGNTSLLVYWVHIEFVYGRFSILPKGQ
                     CSALKATVGLITIFLAMLMLSLLRTNWKKLRLRASK"
     gene            7523..8527
                     /locus_tag="VGR03_16485"
     CDS             7523..8527
                     /locus_tag="VGR03_16485"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEV1995932.1"
                     /translation="MPATRQLGPLPMLGLWAVLTLAGALYSTWLGYGGRAFAATLTAF
                     AFFLLVMLLFAARGVADRLASRFGAGSGYMLGTTVLLVYLIYALGTNTFAFTPAAAVA
                     SLVFIPLALAASAERKPPGAWQDFATILAVWLAVKPMPNPWGWSLSHWLWPYPGGRLA
                     YIFTVLLCVNVALAAFLLLRRVNGVGYSIGWGRHWSFFVLASFVVFGCIAIPLGHAIH
                     FIQFEPQWSQLKSLPFLSLGILLFTAWPEELLFRGLLQNMLSRVSKSDLAGWWTASIL
                     FGFSHITNLGFPNWRYVILASIAGFFYGWTWRKTGSIFASALVHAAVDVTWHFLFRTV
                     "
     gene            8654..9187
                     /locus_tag="VGR03_16490"
     CDS             8654..9187
                     /locus_tag="VGR03_16490"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV1995933.1"
                     /translation="MAETMTAEQAKFLLQDVYLGALKNESRTTKKILEAVPADKAEYR
                     PDPISKSAIELVRHIAAADNRFVETVINGVFDTTTGAIPENVKTPAEIASWYEARYAK
                     NFEALAKLTSEQLLKIVDFRGMFQRPAVTFLMLGLHHTIHHRGQLSSYLRCMGAKVPS
                     IYGESYDAEQARKAAQA"
     gene            complement(9210..9986)
                     /locus_tag="VGR03_16495"
     CDS             complement(9210..9986)
                     /locus_tag="VGR03_16495"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEV1995934.1"
                     /translation="MADELVIAGRAFKSRLIVGTGKYRSFQEMARAHAASGADMVTVA
                     VRRVNLTDKSKESLLDYIDREKIFILPNTAACYTADEAIRTARLGREVGLSNWVKLEV
                     IGDQQTLFPDTEQLIEATRVLVKEGFVVLPYTNDDLIAARKLIDAGAAAVMPLAAPIG
                     SGLGVQNPANLRILRERITEVPMIVDAGVGTASDAAVAMELGADGVLMNTAIAEAQDA
                     VLMAEAMRDAVIAGRKSYLAGRMPKRLYASASSPLCGVVR"
     gene            complement(10101..>11170)
                     /locus_tag="VGR03_16500"
     CDS             complement(10101..>11170)
                     /locus_tag="VGR03_16500"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV1995935.1"
                     /translation="SVEAVAQALLEVHPTITAAVPRFFEKIYARIVEQGSKTTGTKRT
                     IFDWAMNVAQRATPWRSTGSPASLALKAQWKLADKLVYKKIRLGMGGQLRIVSSGGAP
                     LSQSLAEFFWTVGIPIYQGYGLTETSPIVSSNYPANRMGSSGKPIPNVQVRAAEDGEI
                     LVKGPCVMQGYYKNPEATREVLSEDGWFRTGDIGYVDKDNYLFITDRKKDLLKTAAGK
                     FVAPQPIENALKTSPYILNAMVVGDRRKFIVALVVPNATTVAAKAADQGIKFASNAEL
                     AAHPWVHALIDSEVKRLTVHLAQYETIKRFALLPEDFTFDNGSLTFTLKLKRRVVEQQ
                     FAGAIESLFADVAEPRPILQE"
BASE COUNT         2303 a         3333 c         3173 g         2361 t
ORIGIN      
        1 caatggtgat ccgtgcctgt acatcgcttg cgggaggcgg agtttgcggt ttggacgccg
       61 atgccggtgc atcctgggcg gctacccctt gagcaatcac cgcacaggca gcgaggcaaa
      121 cgcatgcaaa aaaaatactc ggaaatctca taaatcggtg aactccatgg tctacggaac
      181 ggcatgctag gtcaagtctc gccgcgttaa gattggatgc cactaccact taaggtgctg
      241 ccccggcgct gctccgccgt ggaaaccccg agcaacagcc atttgactca ttaatagggc
      301 tcctgtaact tctcaagaaa ctgctcggca ggcaagatgg ggacgcttca agaatgaaat
      361 tcaccccgaa cggtcagcgc cggacgccaa actgactgca tcacgctcag aacaggaatc
      421 agaaggagag cacaatgggt tttgccacca acgccattca tgtcgggcag gaacccgatc
      481 ccgccaccgg ggccatcgtc gcgcccatct atcaaacttc cacgtacatc aacgaagagt
      541 tgggcaagaa caaaggttac gactacgcgc gaacgaacca cccgaaccgc aaagccctcg
      601 aacgcacggt tgccaagctg gaaggcggcc acagcgccta cgttttctct tccggcatgg
      661 cgggaatcga cgcggtcttc cggcttctcc gtccgggcga tcatgtggtt ctctccgaag
      721 cggtttacgg cggcgtgttc cgcctctcca cgcaactcct ggtgcacttc gggctggaat
      781 tcagcttcgt ggacacgtcc gtccctgatt tcgtgcgcaa cgcgttgcgt cccaatacga
      841 aaatgctgta catcgagacg cccgctaacc ccacgctcag cgtcaccgat atcgccgcga
      901 tttccaagct ggccaacgag cgcaaccttt cggtggtggt cgataacact tttctgagtc
      961 cttatttgca gcgccccatc gaactcggcg cgcacatcgt ggttcattcc atgacgaagt
     1021 atttgaacgg ccacagcgac tccactggcg gagccgtggt gctcacgcgt cccgaggatg
     1081 cggaaaagat ttatttcgtt cagcgctccg ccggcacggg cctggcgccc atggattgct
     1141 tcctcatttc gcgcgggatc aagaccctgg cagttcgcat gctccagcac aacgccaacg
     1201 gcatcaccgt agcgcgccat ctcgacgcgc atcccaaaat ccgcaaggtc ttctaccccg
     1261 gcctgccgtc tcatccgcaa cacgaagtgg cccgccgcca gcagaaaggc ccgggcgcga
     1321 tgctctcctt cgatgtaggt tctctcgaag ccgcgcggcg attcctcaat cacgtcaagc
     1381 tctgctcgct cgccgaaagc ctgggtggcg ttgaaacttt gatttcgctt ccggcgatga
     1441 tgacccacgc ttcgatgccc aaggaagttc aggatcgcgt tggcatcacc gagggcctcg
     1501 tgcgcctctc cgtcggcatt gaagacgtcg aagatatcat cgccgacctc gatcaggcgc
     1561 tcctctacgt ctgagacctc cacaaattcc cgggtcgcgc gaaggcagtc tgtcattccc
     1621 catccttggt aagtgggtca gtctccgacg aactgaccat gcgtccatgc aaccgccgtg
     1681 caaatcggct ccggcgataa cttccggctg cgcgaccatc cgggagtcat cgcgtggcgg
     1741 ctccaatcga aatgccgagt acaggcactc ctacgggtag cccgttctac gtcaacttgg
     1801 actgctcgat gcagcgcccc ccattcgtaa acacttcggg actttgcagt agcttttctg
     1861 tttccgtgaa ggaagtaaat gccactcagg ctgttaaggc tggaagttat ctactagacc
     1921 gaattcttat ctgggaacta ggtatttccg ccagacgttc atctcgcggc acacgaacat
     1981 agctgggaag aatcttctgt gcgggcctat ttaattcact tcattgatgt aacttttcgg
     2041 ttgttcgtgg atacctattg cgagagaacg tgcttttccc gattgggcat cgagaactct
     2101 tcgaagcagt ccggatcagt ttgtcccgcg ctaaggagca cctatggcga cgctcaccgc
     2161 acccagaatc gaatccgcaa acgcaaagct cgtcgcgccc ccttggcaca cggtagtatt
     2221 agtgactctg tttttgatct tggcgcttgg tggggcgttc tttcaacgag agacccgcac
     2281 acacccggga attttgcagc agcatcctca cgtcgcgccg ctctacttct atctcatcgc
     2341 gatggaatgg gggcttttcg tgtatgtatg gaagggcggg ttgcgccgca ccggaacaaa
     2401 actcagcgaa ctcattggcg ggagatggac aagcgcaaga gaagttctcg tcgacgcagc
     2461 cctcgctctc ggactgtggg gcatttggac actcctggag atgatctggg ttcgtcggct
     2521 cgggcccgac catgccgctt ccatccagac gctgctgcca cgacatgctt tggaaatcct
     2581 tctttggatc gccgtttcca tcagcgcagg tatctgcgaa gaatttgtct ttcgcggcta
     2641 cttccaaaaa caattcgaag cgttcacgca taggaaatgg atagctttgt tcttgcagtc
     2701 tgtgctcttc gggatttccc acggctatca ggggatcgaa gcgtgcgtaa agatcgccat
     2761 cttcggctcc ctgtatggct tgctcgctct ctggcgcaag agcctgcgcc ccggcatgat
     2821 ggcgcatgcg tggtcggaca ttctgagcgg catcttcgga atttgattag ctgtgcacaa
     2881 aaagaccttc atcggtgtaa ctttctggcc gcacatggat aactcttcag agatggatgt
     2941 atgaccggct tcggcgcgct ctacaaaaag tatgcgccgg acgtgtacca ctttgcgctg
     3001 tatctttcgg gcgagcgcgg agaagccgag gacatcacct cggagacgtt tgtgcgcgcg
     3061 tgggcttcgt ccgagccgat caggatggcc acggttaagg gctacctctt cacgattgcg
     3121 cgcaatctgt tcctgcaagg gctccggaaa aaatcgcgtc acgttgtgct cgacgaagac
     3181 ctacgcgatc cgcaggccag cccttatgcc caagccgagc agaaggcaga gctgcgtgcc
     3241 gtgctcgcgg ggctgcagaa gttgccggaa atcgaccgcg cggccttgct catgagggcg
     3301 tttgacgagg tgccttacga agaaatcgcg cgggcactgg gaatttcgct ggccgccgtg
     3361 aaagtgaaga tccaccgcgc gcggctcgtg cttgcagaca ttcgcaacgc ctagcgtctt
     3421 ggggtggatt cgaacgattt ggaggaaagt gatatgaacg tcacgcggga agttattctg
     3481 gatctattgc cggtgtatct ttcgggtgag gcgagtccgg ccacgcgcgc tctcgtcgaa
     3541 gaatatatga agcaagactc ggagcttgcg cagcggattc gtcttcaatg ggcggagaac
     3601 ttcgccaagg tcgcgccctc cgcactacct ccggatctcg agttgcggtc ccttctccgc
     3661 acgcgaagac tcatcggctt gcagcggtgg ctgtttggct tcgggatatt cttcactgcc
     3721 ctctcactca gcaatgaatt ctcgtttgcg ggcgggcacc tcaaggaatt tcatttctta
     3781 ttgcgcgatt atcccgttga gttcgggatt tgcctgacgc tcggcctcgc ctgctggacc
     3841 gtttacttct ccatccggcg ccacctccgg accagctgac acagataggg aatgtcaaaa
     3901 cgcggcagcg tttaccgata cagtccactt ggacgtttag gcagcaaacc ggacgtgggg
     3961 ccattcctgt tactttggtg attgtgcttg gatcacggcg cactcggaag tttacggcgt
     4021 ggtcagcgtg gacttcacga ttctgggcgg attcacggcg ctgctacttc tcgtggcgct
     4081 cggtgcaggt tacctccctg cacggcgcgc gatcctcgtc gacccgaagg tttctctgcg
     4141 ctacgaatag cgagtgctgt tctagctagt cgggcttttt tcggattttc gtgacgctat
     4201 cctccagcca cgaaagatat tttgtcgagc cctcggtaat cggcaacgcg acaaattccg
     4261 gcacatcgta actgtggaga cgcttcactt ccttttccaa ttcggcgaga cgttttgcgc
     4321 tcgtcttcat caccagcaga tactcccgcg ctttttccag ctcgcccttc cacgtataaa
     4381 atgactcggc tgggctcaaa ataatattga cgcacgcagc gagacgcttg ccaaccacgc
     4441 tgcgcgaaat tcttcttgct tccatcagcg ttccgcatgt caccaacacg gcgcgatact
     4501 ttcccgcgac tttcatcact tccccccgtc ttgagtccgg ctgcttcccg gcaatttcat
     4561 cgccgcaggc cgttcgttgc gccggcggtg cgaattgctt ttgtcttccc tcaactcaga
     4621 ctagaatacc gctccatgac gacctctccc atcaagtttg gcacctcggg ttggcgcggg
     4681 ctcattgccg acgattttac tttcgccaac gtgcgcttgg ctgtgaccgc cattgcggtg
     4741 catgtgaaaa ccaaggccag gcagcccacc atcctcatcg gctacgacac gcgcttctac
     4801 tccgaagaat tctcccagct tgccgtagac atcctgcagc accacggcat tcgaacgctg
     4861 ctgtgcgaga ccttcacgcc tacaccagcg atcgctttcg aaatcatgcg ccgcaaactc
     4921 gacggcgcca tcaacttcac ggccagccac aatcccgcgc aatatcacgg cctgaagttt
     4981 tcttccgccg acgcgggccc cgcgctgccg gaagtcacca aggatatcga agcccgggcc
     5041 gcgcagctcg ccgcgaaggg cggtgttccg gcccttccgc acggtgcatc gaagtctgcc
     5101 cgcgacgaaa aagtcaatct gcgcgaaaac tatttgaagc gcctcgaaga gctcgtccat
     5161 ttcgaaacgc tccgcaaggc caagccgtct ctggtcgtgg acgcgctcca cggttgcggc
     5221 gccggctatc tggatcgcat actcaccgat cacggagtta cggtgcaagc catgcgcacc
     5281 gaacgcgatt gcctgttcga cggaaccggg cccgacgtct ctgaagaaaa tctcgcgccg
     5341 ctgcgcaaag ccgttcgcga ttcgaacgcc accgccggcc tggccaccga tggcgacgcc
     5401 gaccgcttcg gcatcgtcga tcgcgacgcc acgtggatcc agcccaatct gattcttgcg
     5461 ctggtgtacg actatctcgt cgaaactcgc aagtggaaaa tgcccgcggc gcgtagcgtc
     5521 gcgacgacgc aaatgatcga cgcggtggcc aagtcgcacg gccaaaccgt tcatcagact
     5581 cccgtgggct tcaaatacat cggccaactc atccgcgaag acaaaatcgc gctgggtggc
     5641 gaagaaagcg ccggactcac catccgcggc cacatcccgg aaaaagacgg cattcttgcg
     5701 tgcctgctgg tggcggagat gatcgccgcg cgcggcgcct ccatcggcga gcaagttcgc
     5761 gcgatgtaca agaaaatcgg ccgcgaattc tggcccaccc gcgagaatct gcatctttcc
     5821 gacgaacaaa aggcaaacgc agtcagacgg gtggccgtgg acgcttcgac actcgccggc
     5881 cggaaagtcg tatccatcga ccgtaccgat ggcgcaaaat ttgtgtttga cgacggttcg
     5941 tggatgctgc tgcgtctttc cggcacggaa ccgctgcttc gcctgtacgt cgaagccgaa
     6001 agcgccgccg cttccgcgaa gttgacgcgc gaagcatcac aatgggtact gcaaacctga
     6061 acaagatggg tgccaacgaa aaaatccagc cgcttctgcg ccactacggg cccgcgcttc
     6121 tcggcctgct cgtgctggtc ctggtggtgc acgacatttt cggcacacac ggttatctgg
     6181 ccatgcgccg cacccaacag gaaatcaaaa aagtaaccgc cgatctggat cagttgaaca
     6241 aggaaaattt gcagctcgag caggaagtga gagagctgaa gaccgatccg cacaaaatcg
     6301 aaaaaatcgc acgtgacgag cttggcctcg ccaggcccgg cgaggtcatc atcaaaatcc
     6361 cgcggtcgca gcagttgccc caggatccta cttcgaagcc ctgagccgca actttttcca
     6421 atttgtcctc aacaaagaca gcatcagcat cgccaggaaa atcgtaatga gtcctaccgt
     6481 ggccttcagc gcgctgcact gccccttcgg caaaatggaa aatcttccat acacaaattc
     6541 gatgtgcacc caatacacca gcagcgaagt attcccgagc tggatgatgg ggctaaaacc
     6601 cttttgcgca aatccccacc ggcaccacgc gtaaaccagg aaaagaatga tcagcaagat
     6661 gccgcagcgc atcaggagga aattcggacc gctgtgccag tagtcgtaaa ccgcgtacag
     6721 ccgcacgcgc aacgaatcca acaaaaccga cgagtagcac gcaacgattc ccgtgccgcc
     6781 cagtgccgcg aacgtcagcc cttccatgcg cttggcaaaa tccgaaaaca gcagaaatcc
     6841 cacggcaagc cccgcgaacg caaatgctga ccatggaaac aagggaaaca gccacggctg
     6901 cggctctttg aagatgtaca cgccgttgat gtaggactcg atgggccagg gcaaaaaatt
     6961 cagccgatgc gtcgtccaaa gcggtggcgt ggccatggcc acagccaccg ccacaaaaat
     7021 ccccgccacc tgagtgcgaa atctcgccac ggcgacggcg ccatctccgg cggcccagca
     7081 cagcacaccc atgagcatca tggaaagccc gagaatgttt agaacgtcga cgcgcagcag
     7141 atccgtccac ggcgaccatg gataaccgag cgcaaattcc tgcacgcgaa acagaagacc
     7201 catcccaaag acttccgcgc cgcgcagaat cgtttgcttg gcgatggcgt tgcgcttgat
     7261 tcccttttcg cgcaattttt ccgtcaccag agcgaacgac acgcccgcca agaaaatgaa
     7321 aagtggcgca ggcagcgtcc cgcccagccg tgaccaggca atcagtgacg acgatttctt
     7381 ggcctccggg ctcatccagg agtcgtaaca gtgtgtctga aacatcagca cgcacgcgag
     7441 gccgcgcatc cagtcgatgt aggcgaggcg tctggccggt gattgaattt ggtctgccat
     7501 ctccctgcgt tacaattctc gcttgcccgc cacgcgccaa ctcggccctc tccccatgct
     7561 cggtctctgg gccgtgctga ctctagccgg agcgttgtat tccacttggc tcggctacgg
     7621 cggccgcgcg ttcgctgcca cgctcacggc cttcgcgttt ttccttctgg tgatgctgct
     7681 ttttgccgcg cgcggcgttg cggataggct ggcctcgcgc ttcggcgccg gcagcggcta
     7741 catgctcggc accacagtgc tccttgtcta cctgatttac gccttgggga caaatacttt
     7801 cgcgttcact cctgccgcag ccgtcgccag tctcgttttc attccgctgg cgctggccgc
     7861 ctccgccgag cgcaagccac ccggcgcatg gcaggatttt gcgacgattc ttgccgtttg
     7921 gctcgccgtg aagccaatgc cgaatccctg gggatggtca ttgtcacatt ggctctggcc
     7981 gtatcctggc gggcgcctcg cctacatctt cacggtgctc ctctgcgtta acgtcgcgct
     8041 ggcagccttc ttgcttctcc gccgcgtcaa tggcgtcggc tactccatcg gctggggccg
     8101 ccactggagc ttcttcgttc tagcaagttt cgtcgttttt ggatgcatcg ccattccgtt
     8161 gggccacgcg attcacttca ttcaattcga accgcagtgg tcgcaattga agtcactccc
     8221 gtttctctcg ctcggcattc ttctttttac cgcgtggccc gaagagcttc tcttccgcgg
     8281 tttgctccaa aacatgctct cacgcgtatc gaagagcgat cttgccggct ggtggaccgc
     8341 ctccattctc tttggcttct cgcacatcac caacctgggc tttcccaact ggcgttacgt
     8401 gatcctcgct tccatcgccg gctttttcta cggctggacg tggcgcaaga ccggctcgat
     8461 cttcgcctcc gccctggtcc acgccgccgt tgacgtcacg tggcatttcc tgttccgcac
     8521 ggtctagtct tgctgccgca ttccatgaaa cggaaacttg aatcttgcat catacttggc
     8581 aatgcggcgc ggggcaggcc cagtgcccgc cccgcgccca ccgagaactc gaaacgcatt
     8641 caggaggaga gcgatggcgg aaacgatgac ggcagagcag gcaaagttcc tgcttcaaga
     8701 cgtgtatttg ggtgctctga agaatgagag tcggacgacg aagaagattc tcgaagcagt
     8761 gccggcggac aaggcggagt accggcccga cccgatttcg aagagcgcga tcgagctggt
     8821 caggcacatt gccgcggcgg acaatcgttt tgtcgaaacc gtgatcaacg gcgtgttcga
     8881 cacgaccaca ggtgcgattc cggaaaacgt caagacaccc gctgaaattg catcgtggta
     8941 tgaggcgcgt tacgcgaaaa attttgaagc gctcgcaaaa ctaaccagcg agcaattact
     9001 gaaaatcgtg gattttcgcg gcatgttcca gcggcccgct gtgacgtttc taatgctcgg
     9061 cctgcaccac acgattcatc accgcggcca gctctcttcc tacctgcgat gcatgggcgc
     9121 aaaagtcccc tcgatttatg gcgaaagcta cgacgccgag caggcacgca aagccgcgca
     9181 agcgtaaata gttttctcac gagaaatcgt cagcggacaa cgccgcagag cgggctggag
     9241 gcggaggcgt agaggcgctt gggcatgcgg ccggcaagat aagatttgcg gccggcgatg
     9301 acggcgtcgc gcatggcttc ggccatgagg acggcgtcct gggcttcggc gatggcggtg
     9361 ttcatgagga cgccgtcggc gccgagctcc atggcgacgg cggcgtcgga agcggtgccg
     9421 acgccggcat ccacgatcat gggaacttcg gtgatgcgtt cgcggaggat gcggaggttg
     9481 gcgggatttt gcacgccgag gccggagccg atgggcgcgg cgagaggcat gacggcggca
     9541 gcgccggcgt cgatgagctt gcgagcagcg atgaggtcgt cgttggtgta agggagaaca
     9601 acgaagcctt ctttgacgag cactcgtgtc gcttcgatga gttgctcggt gtccgggaaa
     9661 agagtttgct gatcgccgat gacttcgagc ttgacccaat tcgagaggcc gacttcgcgg
     9721 ccgaggcgcg cggtgcgaat ggcttcgtcg gcggtgtagc atgcggcggt gttcggcaag
     9781 atgaagattt tttcgcggtc gatgtaatcc agcagtgatt ccttcgattt gtcggtgaga
     9841 ttcacccggc ggacggcgac ggtaaccatg tccgcgccgg aagcggcatg cgcgcgagcc
     9901 atttcctgaa agctgcggta cttccctgtt cccacgataa ggcgggactt aaaagcgcgg
     9961 cctgcgatga ccagttcgtc tgccatgatg acctcgaaat gattttagac ctccgagggc
    10021 gcgggaacaa atcgtgcgaa aggattcaac acaaaggtca cagagaacaa aaatttcacg
    10081 gagcaaaaaa ctgccgaaat tcattcctgc aaaatcggac ggggctcggc gacgtcggcg
    10141 aagagggatt cgatggcgcc ggcgaactgt tgctcgacga cgcggcgctt gagcttgagg
    10201 gtgaaggtaa ggctgccgtt gtcgaaggta aaatcttcag ggagaagagc gaagcgtttg
    10261 atcgtttcgt attgcgcgag atggacggtg agacgcttga cctcggagtc gatcaacgca
    10321 tgtacccagg gatgggcggc cagctcggcg ttggaggcaa atttgattcc ctggtcggcg
    10381 gctttggcgg caacggtggt ggcgttgggg acgacgagcg ccacgatgaa tttgcgtctg
    10441 tcgcccacga ccatggcgtt caggatgtaa gggctggtct tgagcgcgtt ttcgatgggt
    10501 tgcggggcga cgaatttgcc cgccgcggtt ttgagcaaat cttttttgcg gtcggtgatg
    10561 aagagatagt tgtccttgtc cacgtaaccg atgtcgccgg tgcggaacca gccgtcttcg
    10621 ctgagaactt cacgggtggc ttcgggattc ttgtagtagc cctgcatgac gcaagggccc
    10681 ttgacgagga tttctccatc ctcggcggcg cgaacttgga cgttcggaat gggtttgccg
    10741 gaactgccca tgcgattcgc ggggtagttg ctggagacga tcggagaagt ttccgtcaga
    10801 ccgtaaccct gatagatcgg gattccgacg gtccagaaga attccgcgag cgattgggaa
    10861 agcggagcgc cgccggaaga gacgatgcgc agttggccgc ccattccaag gcggatcttt
    10921 ttgtaaacca gtttgtcggc gagcttccac tgcgctttca aggcgaggct cgcaggggac
    10981 ccagtggagc gccacggagt ggctcgttgg gccacgttca tggcccagtc aaaaatcgtt
    11041 cgtttcgtgc cggtggtttt cgagccttgc tcgacgatgc gagcgtagat tttttcgaaa
    11101 aaccggggta ccgcggccgt aatcgtgggg tggacttcga ggagagcctg ggcgacggct
    11161 tcgacagact
//
LOCUS       DASYVO010000481        23312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_208805, whole genome shotgun sequence.
ACCESSION   DASYVO010000481 DASYVO010000000
VERSION     DASYVO010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 23312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23312
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208805"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VGR03_16505"
     CDS             complement(<1..488)
                     /locus_tag="VGR03_16505"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEV1995936.1"
                     /translation="MSKRFFSAGCILAVVGVLAVALAGAALRASSGAADKPNSWGFDT
                     ANLDKTCKPCDDFYQFAMGGWMKSNPIPPEYSNWGTFTQLADKNQQNLRQILDAAALA
                     KVVAGSNEQKIGDFYASCMDTTAIDVAGMRPIEPELAHIAEIKNVSDLQAEAKRLHSR
                     GVG"
     gene            complement(644..2383)
                     /locus_tag="VGR03_16510"
     CDS             complement(644..2383)
                     /locus_tag="VGR03_16510"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1995937.1"
                     /translation="MAIEQQLDAAGSPRKGHVIFLLLAVLTLGSSAFAQDHSPHQARL
                     VEAQSAFDAGRWDEALRLAQGPADQSPDLDFVAGLALARMERWSEAKLVFESGVRKAP
                     SDSRFRAELAGIAYKQKDFRTAKDNLHAALRLNPQDAYSREFLATIYFLEGNLEAALQ
                     YWNPEDKPRLRSVAFAPSLQLKESLRNRALAFNAPQVLTSDALLATQSRLDNLGVFSN
                     RRIELNPGESGNYDLTLHLAERNGWGDSKVEGIVSLLSGLPYATVYPELYNLGRDAMN
                     LTSLARWDSEKRRLSLALSLPLYGDPSLRLRLYADARNENWNLAQPFFGAGTLLADLN
                     MRRAAAGAEAHSIVDGRWSWSAGAEIANRNFRNLGSHTSPAERAFFTGATSVAGWLGV
                     KRTLLRVPERRFTLDSSAEAKAGREFADGLGPFVTLRGSLRAHWFPRARGDDYEVQAQ
                     IRAGATAGKATLDELFELGVERDNDLWLRGHAGTLNGRKGAAPLGRRFFLANWEMDKN
                     IYQNGFFTVKLGPFLDNGAVADSSGLFGSQRWLWDTGAQCKVRVLGSLTIVLSYGRDL
                     RGGRNVFFGTVLR"
     gene            complement(2329..2886)
                     /locus_tag="VGR03_16515"
     CDS             complement(2329..2886)
                     /locus_tag="VGR03_16515"
                     /inference="COORDINATES: protein motif:HMM:NF023802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEV1995938.1"
                     /translation="MNRIALGLLLLLFLGRCIHPAPQEKAGIWLDVPFVKQSEDGCGS
                     AAISMVLQYWNAHGARVDPRRADAVAIQRLLYSRNARGIYASDIESYLKGLGFRVFSL
                     QGEWNDLRERLTQGRPLIVSLQPGNAKAPLHYVVVTGIDWRNGAVFINDPARGKLIRI
                     ERAEFEKEWRSNSNWMLLALPEKAM"
     gene            complement(2918..3322)
                     /locus_tag="VGR03_16520"
     CDS             complement(2918..3322)
                     /locus_tag="VGR03_16520"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HEV1995939.1"
                     /translation="MRFCVKMCVPVLAIALLLGLPGAPAGAQDKQHVVSLSELNKDAA
                     RPAQTRQANEEAVRTLLSSDQAQKALKSADLDYQKVDKAVGQLSDEDLAKLAERSRQA
                     QNDFAAGRISDRDLLWIILIAIGIIVLAVVLR"
     gene            complement(3428..3925)
                     /locus_tag="VGR03_16525"
     CDS             complement(3428..3925)
                     /locus_tag="VGR03_16525"
                     /inference="COORDINATES: protein motif:HMM:NF041379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYCXC family (seleno)protein"
                     /protein_id="HEV1995940.1"
                     /translation="MRGNEIWRFLSAAALCGFALVLVLVPQQAASKSPQTTSLPQPAA
                     DEPVPAYHEQAPEGALPATMNPELFTDPVVQNAYAVAAKIKKTLYQQPCYCHCDRSQG
                     HGSLFDCFASKHGAGCNICIYEDFYSYEQSRKGKTAAQIRAGIIKGDWRSVDATKYQK
                     QLPAK"
     gene            complement(4133..4831)
                     /locus_tag="VGR03_16530"
     CDS             complement(4133..4831)
                     /locus_tag="VGR03_16530"
                     /inference="COORDINATES: protein motif:HMM:NF024197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HEV1995941.1"
                     /translation="MAGLHNSVKGSLAGRTFSRFLRFGVLRGLRSIEIDPEDFRRHLA
                     NKHGLWVPHFARMKDVPTERLDAIAKTLIRDAERLALAEGAGFGLGGMITFLPDASLL
                     TVITLRLIQRLALLYGFEAHGRDQRIEMWKAAAAASGIDYGKDLAEKQIFEKLAPRIA
                     ERFAAKIGAETAEKWAGRLIPLASSAIGGALNFSFVRGWGRRVQRHLRAKHIEAKAST
                     PPPAYTAKVEPAFI"
     gene            5009..5296
                     /locus_tag="VGR03_16535"
     CDS             5009..5296
                     /locus_tag="VGR03_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995942.1"
                     /translation="MATMPQFVPSEVVHDLDFPQREAAFFYGLFLRGYSADQLRRDIE
                     VPPVVLAKWHREAERDPQLRDIFTRMVDYRRHVLAIFDTLVGSDGNPQRVQ"
     gene            5543..7246
                     /locus_tag="VGR03_16540"
     CDS             5543..7246
                     /locus_tag="VGR03_16540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEV1995943.1"
                     /translation="MSRVVRTLFVSLFLMAIAIPSVSAQQAQDPGTPPAPQKMDKEAK
                     RKMRKTLKELGFEYRQWLTEDFTYIISPDERNAFLQLDTNEEREQFIEQFWLRRSSNP
                     DLPENDFKEEHYRRIAYANEHYASGIPGWKTDRGRMYIMWGPADEVESHPTGGTYDRP
                     MDEGGGSTTTYPWETWRWRYLEGIGENIILEFVDPSGSGEYRLTMDPSEKDALLHVPG
                     AGLSLMESMGMASKADRFTRADGTNLPRSMGGQPASMNEFSRLELYAKVNKPPEVKFK
                     DLEAVVTSRIVRDQVHFNWRTDYLKVTNDTVLVPVTVQVPNSQLSFQAKDGIHSAVLN
                     IFGRVSTLTGRVVQTFEDSVTRDFPDSLYQQSLKLQSIYQKAVPLRPGLYRLDLVIKD
                     VQSGNIGVVNSRLQVPRYDDDKLEASSLILADQIEHVPAKQIGSGQFVLGSSKVRPRL
                     GGDFTTADKLGIYMQVYNLKPDDKTHKSSGTFQFTVKKGDQIVPGMQFIITTEEMKQT
                     GDQVTIERLLPLATLPPGKYKIEVNATDTLSNQTISRSAEFTVKAPPETKTAANAAPG
                     R"
     gene            7253..8998
                     /locus_tag="VGR03_16545"
     CDS             7253..8998
                     /locus_tag="VGR03_16545"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV1995944.1"
                     /translation="MKQVRLRILGALVLTVMVSSFAAIPAAAQAKPVPGKLAGVVRDA
                     AGTPQMGASVELMSEAVGVAASRGFLTNTQGMFRGDKLAPGFYTVRVTLAGFLPTLEK
                     HIRVTSNLTTVVRIELESMFASLDQLRRMPSNSTAEVDDWKWVLRSASAMRPVLEWVH
                     HDTLSVSTLTGESGRVRAPRIRMEFTDGARRPSSASSIASSPATAVAYDQKLGGTSRM
                     ILAGQVSYDGDSPAGGIATVWLPTGALGAGPHTALVLREAKLGPVGPTFRGIRFDQGG
                     VLTLGDRTVLRYGGEYVLVGLGAAASSLRPRAELNVRLTDDWSTAIVFASMPNGPGPL
                     EASDAQPGGALAAALNELDAFPALLWRQGRPVLQSGLHEEISAERKISTRGKLQIAGF
                     HDDNRHVAVFGRGSDLPTADYFQDYFSTGFAYDGGSSSSWGTRVAFREKLDGDVELTA
                     VYSFGGALTPSDDADGLLRDALRTAQRHSLGAGISAKVPRIGTKVQAGYKWVSGITVS
                     SVDSYGESLFQMDPYLHLIVRQPLPKFVLGRWEAIADCNNLLAQGYVSINSRDGRVVL
                     VPAFRTFRGGLSVQF"
     gene            9184..12003
                     /locus_tag="VGR03_16550"
     CDS             9184..12003
                     /locus_tag="VGR03_16550"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1995945.1"
                     /translation="MRQNLRVSLGAVVLALATLAAVIFALINFDQRSRFEVVYDGVAW
                     LDTDHGIQASRVSPNSPATRAGIRPGDVLLTINSASVTRAAEVARRLDRAGLWTQVRY
                     KLSRAGEEFETPILTAPAEKPLATENYLRIVGLLYLFIGLFIFIRRWNAPRAVHFYVF
                     CLVSFILWSFHFSGKLDTFDWEVYWSEIVARLLAPALLLHFALVFPGRSETTIRSSSK
                     LLAVYSLPVALLLIHLSTALNALGFVPWLGAYLLLRKIEFSYLALCFLAAGLVFYRSY
                     REAPSGVLRQQLKWLTGGTLAGSLPVSLLYIVPLVSGVALRPWMQLSVLSLVLIPLCF
                     GYAVIRYRLMDVDIIFKRGLAYTAATAGVAAVYFALVALIAGIFHAPTSGPVGGMIAI
                     VIAAFLFQPLRAGIQARLDRFFYRDRLDYRRTLIEFGRTLTNEVRFDPMLGSVMDRIS
                     QTLLVDRLAIFVESGLQPGQMRIARSMGVRLSESLDLSFLEPARPEFARGALFFESPR
                     AARDVSDSVRRTLEQLDLNYFVPCRIREHTVAVLGLGKTVDGDFLSSDDVELVETIAG
                     YVAVALDNAQLYSSLEQKALEIARLKDFSENIVESLNVGVLSIDLEGIVESWNTRMEQ
                     LFGVVRQEAVGRQLSSLLPEELAREIAARGDQEQITGIYKQRLQHQGKLLTMNVSITP
                     LVSKSNERIGRLLLFDDVTQRERMEEQMSQTEKLTSLGLLAAGVAHEVNTPLAVISNY
                     IQMLAKQMPEGDPRQSIIEKIVKQTFRASEIVNNLLNFSRTGAAEAADIDVNRVVEET
                     LSLVAHPLKSSHIQVVKQLGEPLPAVRGSANKLQQVFLNLFLNARDAMPSGGMLEVRT
                     AAHNGSVEIEVADTGAGIPREHIHRIFDPFFTTKASGRGTGLGLSVSYGIIKEHAGKI
                     DVRSTPGKGTSFHVEFPAVRKAVHV"
     gene            11996..13468
                     /locus_tag="VGR03_16555"
     CDS             11996..13468
                     /locus_tag="VGR03_16555"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV1995946.1"
                     /translation="MSKGSILVVDDESEIREGLELLLRGEGYGVSSAETGESGLEKLE
                     QHPYDLLLLDVSLPDRNGLDMLKEIHRRDPQLSVVLITAYGSIDMARAAFKNGAMDYI
                     TKPWSNDELLAQVAQAVESRRLRDENVQLKRALKQRFNFPNIIGKSDKMQVLLNLVTQ
                     VAPSRSTILISGESGTGKELIAKAVHSASTRADKAFVPVNTGSIPVDLLESQLFGHVK
                     GAFTSAVASKKGLFEVADQGTIFFDEIATISPETQAKLLRVIQEREFMRLGGTEQLKV
                     DVRIVAASNVDLLTLVREGRFREDLYHRLNVIHLQIPPLRDRREDVPLLLAHFLERYC
                     QENNKPLRQFTQVAMKLMMDYEWPGNVRELENVVERAVVLSTQERVDADLLPESIRSK
                     EIVRGVRLQLSEFLPPLPGEPGSRSSADNPQPSLFQIMDEIERRIIIDMLERTGWNQT
                     EASERFLIPLSTLNQKIKRLGIDVRRRGRSDETLSASIGK"
     gene            13555..14736
                     /locus_tag="VGR03_16560"
     CDS             13555..14736
                     /locus_tag="VGR03_16560"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV1995947.1"
                     /translation="MAESTSPFDPADTTSFANEWLALLECASSAHDRERLAGLLRSAD
                     WARLLVLAEEHGVAGHLAACLRGLEEDLVPLEIKQTLIDRQRAQAFFTLRLTAELFRI
                     LDRFTSEGIGALVVKGPVLAMQAYGDPAMRSYGDLDLLVRQRDIRRATELMSAAGFAA
                     AVPLSAIDAGKIPGQYLFSKPDLKLIVELHNDLTLRYFPRRLPLEEFFARQIRVRIDA
                     REALALSVEDELVLICIHGAKHFWERLMWIADVAALVSRQTGIDWERVADSARAVGAE
                     RMLRTGLLLAGDLLKAQLPERAQVMVQADVVAARLAEQICTWLPAAGYSPPGLFERAA
                     FRLRMRGNLIAAPAYLLRLSFSPTEEDWKEGGEGSPHKFLDMVRRPFRLARKYGRDNK
                     S"
     gene            complement(14849..18151)
                     /locus_tag="VGR03_16565"
     CDS             complement(14849..18151)
                     /locus_tag="VGR03_16565"
                     /inference="COORDINATES: protein motif:HMM:NF027116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEV1995948.1"
                     /translation="MKTRGSWVKVAKFVGVTGLVCGLLMVAAFRSARKRSPQTGAGRS
                     QVAASQASKISATKGDPKWSEAYGKLPLSFEENQGQTAREVRYVSHGSGYELFLTSQE
                     AVLALRSKVPRDLSPLRRTATLRALRKARRAGQSTAIRMQLEGANPDAKIAGMDQLPG
                     KTNYFIGNDPKNWHTDVPSYARVKYAGVYPGVDLVFYGNQRRLEYDFVVAPGADPKVI
                     AMKVDGARKMCVNSRGDLVLSVAGGEVELQKPVVYQNVKGERREIAGGYAIDRNRRVT
                     FAVGSYDRTEPLILDPVLNYSTYLGGSAGDVGFAIAVDGSGNAFIAGETMSTDFPAGT
                     KVAFGTGPTPNLGASFVAELDPTGTKLLYSTYLAGTASNRFESANAIAVDSGGKIYVT
                     GGTFATDFPMPTTPNGFKSGSNPGNVNGTSYIAKLDPTVSGAASLLYSSYIGGTNGTA
                     LFGGDFGEAIAVDANGIAYVAGYTDSTASTTVTSMPNFPVVNGFQTTLNSTNGNAFLA
                     KIDTTKSGSASLIYSTYVGGAGANALVAGGLGFGEEAFGVAIGASGNAYLAGQTSSTD
                     FPTFGNAYQPNRPAGNTKATAFVTQIDTTKIGPLSKNYSTYLGGATFDLAYAISLGPG
                     NVAYVTGTTNSNDFPVTPKPGAFDTSGAAGGKAFVSLIDTTQAAVPSLKYSTFLGGTG
                     VDTGFGIRADAQGNAYVAGTTSSLNFPFPPKGMIVGGFEPTYPPGAPSVGFIAKLSPA
                     GNNAADLLYSTYFGGNGNAPNGDHIFAIAIDTSNPANAYVTGQTYSTAATFPVFPKTA
                     PTAFQTTLNGTSDGFVAKLTPIATLTVAPTSLDFGTILIPNTSPAQTVTLTNNTNAAI
                     AFASAVVNGNPAAANTDYKVTNSCSGSIPFGATNTCTVSVTFKPTVVGAETATLQLTD
                     SDSTSPQLISLTGSGTNTPPDFGLTGPTSVQNVTDGSTLNFNVTVTGVGGFSSPVALA
                     CTGAPALATCTIPSSPVTPPVAPPSSVQAQVSMTTTAMVVPPPRVPTLPMSIRQVVPL
                     ILALVLLFLLPRTQQLRTRFAMVTAMMLFVVLAGCSGPAKPHTPKGAATLTITGTSGA
                     LTHTVQVQISVN"
     gene            complement(18440..18880)
                     /locus_tag="VGR03_16570"
     CDS             complement(18440..18880)
                     /locus_tag="VGR03_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995949.1"
                     /translation="MPAYPHLCLWPDSVEMLYGSPDALPRFSTGWEKRRLALGDQGTR
                     FENRSLPLGAIYLLGDRRPDQAPFVEGVRPQAGLLSLVADTFANKILDREMRAREFAV
                     LGRLVTTVPIRRIHPHNDASRLEELCAVIREDFASLRSPTSARL"
     gene            complement(18916..19368)
                     /locus_tag="VGR03_16575"
     CDS             complement(18916..19368)
                     /locus_tag="VGR03_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995950.1"
                     /translation="MGSPSDAADVELHLGVSPNSKSEIPADSEVLTYTSSYTDESGNP
                     ALRIWKTADGGFLRLAYYDGVQFWLEREGKSLWAVWPEASNLEDTASYLLGPVLGLLL
                     RLRGVTCLHASAVSIEKRCVAFVGSEGAGKSTTAAAFARQGLASFLMM"
     gene            complement(19743..21605)
                     /locus_tag="VGR03_16580"
     CDS             complement(19743..21605)
                     /locus_tag="VGR03_16580"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEV1995951.1"
                     /translation="MSGIVGIYHRNGAPMERALLQSLVDFLSYRGPDSRECWMDGSIG
                     LGHAMLRTTRESLGERQPASLDGRFWIAADARLDGRAEFIAELQRSGRVVRLNAPDSE
                     LILHAYATWGTPCVEHLRGDFSFAIWDARNKQLFCARDHFGIKPFYYIQQEDLFLFSN
                     TLNCIRMHPEVSGELNETAIGDFLLFGLNYDNATTSFRDIQRLPPAHSLSISPEGLKL
                     RRYWTPPTDGRIRYSKPEEYVENFQSLLQAAVADRLRTDRVGILLSGGVDSSAVAAVA
                     KEVSAKSAQTTDIRGYTHVFESLIPDREGEYAREVGEFLHLPVKFMASDQTQLFEGWD
                     NPEFSLPEPVEDPLFSGFLDSCRNISADCRVLLSGEGVDNLLEFQMWPYAADLRRSGE
                     WRRLLTEMANYFWIRPFPWRGIRTRMKRIIGKDPDMPVFPPWLNREFLHRAKLKARWQ
                     ELCEHPVMPSRHPIVPNAHASLSLPQWTHMFEQENAGWTPYPLEVRYPFLDLRIVNYL
                     LAIAPFPWFFEKMLLREAMAERIPERVRMRPKTPLQGDPVSAQLQKTGAERLNQMQWS
                     KDSDRFIECSALAAPHGKMNAEQIRTHLRPYCLNIWLQSARRIRYNIYAEAGNG"
     gene            21951..23246
                     /locus_tag="VGR03_16585"
     CDS             21951..23246
                     /locus_tag="VGR03_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995952.1"
                     /translation="MILRLYSNAQRAVLVSASFVVALLLSYFSIRNALATHYAGLQTA
                     EAYERAALLEPANPFNWYLLGRYWQYNLEDPNAARAIRSYLSALSLNPASSETWLDLA
                     TAYESEANPVAARDAFLHAKKVYPLSADVSWRYGNFLLRQGEREPAFTEMRLAVVADP
                     KRAAEAFSRSLRAGSSIETTLDRVLPPISEAYLDVIWDQTTDGHTGNALKVWDRLASL
                     HPRFSLYDSFSLVGALMTEKRIAEASRVWDQAVLFAGLSDLQGPPGSVLWDGGFESNV
                     TAGGFSWLHSEGFRGVQIGMDTQEKHSGNRSLRLIFDGKSNINFTGVCHYVPARPSTA
                     YRFSAWVRTRALTSDQGIRFQLHSLGTQDTSTAVTSEVHGTAPWTRIELPWSSGKDVQ
                     ELQVCLVRFPSQEAENKIQGTAWVDDVALVPEPAEHPKP"
BASE COUNT         4719 a         6941 c         6647 g         5005 t
ORIGIN      
        1 cccacgcccc tgctgtggag ccgcttcgcc tccgcttgca aatccgatac gttcttgatt
       61 tcggcaatgt gcgctagttc cggctcgatc ggtcgcattc cggcgacgtc gatggccgtc
      121 gtgtccatac agctcgcata gaagtcgccg atcttttgtt cgttcgatcc tgctacaact
      181 ttggcgagcg ccgcggcatc gagaatttgc cgcaggttct gctggttctt gtccgccagt
      241 tgcgtgaagg tgccccagtt cgaatattcc ggcggaatcg ggttggattt catccacccg
      301 cccatcgcga actggtagaa gtcgtcgcaa ggcttgcagg tcttgtcgag attcgcggtg
      361 tcgaatcccc acgaatttgg cttatccgcc gcgcccgacg acgcgcgcag agcggcgccc
      421 gccagagcta ccgccagaac accgacaacc gcgaggatgc acccagcaga aaaaaatcgt
      481 tttgacattg tctgcccctg tttggattaa gtttgaaagc gcggcagttg ccacccgttc
      541 aggaccccgg ccgccatgcg atctgattgt gagacggagg aaacggcagt cgggttacgt
      601 cagcccagcg gtcctcaatc ctgcgccgaa acgccggtaa gaatcagcgc agaaccgtac
      661 cgaagaagac gtttcgtcca ccgcgcaagt ctctcccgta ggatagaacg atagtcagac
      721 ttcccaggac tcgaaccttg cattgtgcgc cggtatccca gagccagcgc tgtgagccga
      781 agagtcccga ggaatcggcc accgcaccat tgtcgagaaa tgggccgagc tttacggtga
      841 agaaaccgtt ttgatagatg ttcttgtcca tctcccaatt tgcaagaaaa aatcgccgcc
      901 cgagcggcgc ggctcccttg cggccgttaa gcgttccggc gtggccgcga agccagaggt
      961 cgttgtcgcg ttcgacgccc aattcgaaga gttcatcgag agtagctttg cctgcggtcg
     1021 cgcccgcgcg aatctgcgct tgcacctcat agtcgtcgcc tcttgcacgc ggaaaccaat
     1081 gagcgcgaag cgatccgcgg agcgtgacga acggcccgag accatccgca aactcccggc
     1141 cagccttagc ttcggcggac gaatccagcg tgaaacggcg ctccggcacg cgaagcaggg
     1201 ttcgcttgac gcccagccat cctgcaaccg acgtagcgcc ggtgaagaac gcgcgctcgg
     1261 cgggcgaagt gtgactgcct agatttcgaa aattccgatt ggcaatctcc gcgccggcgc
     1321 tccaactcca gcggccatcc acgatggaat gcgcttccgc tccagctgca gcgcggcgca
     1381 tgttcaagtc cgccagcagc gtgcccgcgc caaaaaaagg ttgcgccaag ttccaatttt
     1441 cgttgcgcgc atcggcgtaa aggcgcaagc gcaggctggg gtcgccgtag agaggcaggg
     1501 aaagcgcaag agagaggcgg cgcttttccg aatcccatcg tgcgagcgag gtcaaattca
     1561 tggcatcgcg gccgagattg taaagctccg gatagaccgt tgcgtaaggc aagccgctca
     1621 agagcgaaac gatgccttct accttggaat cgccccagcc gtttctctcc gccagatgga
     1681 gtgtcaggtc gtaattcccg gactcccctg gattgagttc gatccgccga ttcgaaaaaa
     1741 cgcccagatt gtccaaccgt gattgtgtcg cgagcagcgc atcgctcgtc aggacttgag
     1801 gagcgttaaa cgccagcgcg cggttccgga gcgattcctt caattgtagc gaaggcgcga
     1861 aggcaacgct ccgcagccgc ggtttgtctt cgggattcca atactgcagt gctgcttcga
     1921 gattgccttc caggaaataa atcgtggcga gaaactcgcg cgagtacgcg tcttgagggt
     1981 tcaatcgcag cgctgcatgc agattgtctt tcgcggttcg aaaatctttt tgcttgtacg
     2041 cgattccggc cagctccgct cggaatctcg aatcactcgg tgccttccga actccagatt
     2101 caaacaccag ttttgcctcg ctccatcttt ccatgcgcgc caaagccaat cccgccacga
     2161 agtccagatc gggcgattga tccgccggac cttgtgcgag gcgcagggct tcatcccagc
     2221 gccctgcatc aaaggcgctc tgcgcttcaa caaggcgggc ctgatgggga gaatgatcct
     2281 gtgcgaaggc tgacgaaccc agcgtcaata cggcaagcag aagaaatatc acatggcctt
     2341 ttctgggaga gccagcagca tccaattgct gttcgatcgc cattcctttt cgaattccgc
     2401 acgctcgatt cgaataagtt tgccacgcgc ggggtcgttg atgaaaacgg cgccatttcg
     2461 ccaatcgatt cccgtgacca caacgtagtg caacggcgcc ttggcgttcc caggttggag
     2521 gcttacgatc agcggccgtc cctgggtcaa gcgttcccgg agatcgttcc attcaccctg
     2581 aagagagaaa acgcgaaagc ccaaaccttt gagatagctt tcgatatcgg aagcatagat
     2641 cccccgggcg ttgcgcgaat agagcaacct ttggattgcc accgcgtcgg ctcgccgcgg
     2701 atcgacgcgc gcgccgtggg cgttccaata ttgcaggacc atggagatcg ccgcggaccc
     2761 gcagccgtcc tcactttgct ttacgaatgg cacgtcgagc cagattcctg ccttttcctg
     2821 gggcgccggg tggatgcaac gccccaggaa gagaagcagg agcaaaccga gagcaatccg
     2881 gttcatccgc gtgaggttta actccgaatc gggagaatta gcggagcacg accgccagaa
     2941 cgatgatgcc gatggcgatc aggatgatcc acaacagatc gcggtcggat atgcgtccag
     3001 ccgcaaaatc gttctgagcc tgccgggagc gttcggccag cttggcaaga tcttcatcgc
     3061 ttaattggcc gacggccttg tcaaccttct gatagtcgag gtcggcggat ttcaatgctt
     3121 tttgggcctg gtcggaagag agcagtgttc gaacggcttc ttcgtttgcc tggcgcgtct
     3181 gggcgggccg cgcggcgtcc ttgttcaatt cgctcagaga gacgacgtgt tgcttgtctt
     3241 gggcccccgc tggggctccc ggcaaaccaa gcagaagagc aatcgccagc accggcacac
     3301 acatctttac gcaaaaacgc attcttcctc ctattttgac tgggaaaaac agtttacgga
     3361 ctcgctcgcg ttgtgacaag aggcttcttg gagattttcc gttggggcaa tccctggtgg
     3421 atgcggctca cttcgccggc aactgtttct ggtatttcgt agcgtccacg gaccgccaat
     3481 cgcccttgat gatgcccgcc cggatctgcg cggcggtctt gcccttgcgc gactgttcgt
     3541 agctgtagaa atcctcatag atgcagatgt tgcaacccgc gccatgtttg ctggcgaagc
     3601 aatcgaacaa gctgccgtgt ccctggctgc ggtcgcaatg gcaatagcag ggctgctggt
     3661 acagcgtctt cttgatcttt gcggcgaccg cgtacgcatt ttgcacgaca ggatcagtga
     3721 agagttcggg gttcatcgtc gcgggaagcg cgccttccgg ggcttgttca tgataagcgg
     3781 gcacgggctc gtcggctgcg ggctgcggca gactcgtagt ttggggcgat ttcgaggcgg
     3841 cttgctgcgg aaccaacacc agcactagcg caaagccgca gagcgccgcg gcgctcaaga
     3901 atctccagat ttcatttcca cgcatacttg cccccgttct tcgctcaacc gcagcatcgt
     3961 cgccgccgct ctactctaag gctgccaaac gcgaatttcc acttcgtcgc tcgtgagatt
     4021 catttaagag aaacacgcac tgtaactcat cgccgccagg cagagtgcag gaatttcatg
     4081 gcaggagccc ttcacgggcg gaggccggca atttgctctc atgcgggggt tgctagatga
     4141 acgcaggctc aacttttgca gtatacgcgg gaggcggcgt ggaagctttc gcttcaatgt
     4201 gctttgcacg aagatggcgc tgcacgcggc ggccccagcc cctcacaaac gaaaagttca
     4261 gcgcgccgcc gatggccgag ctggcgagag gaatcaatcg cccagcccac ttttccgccg
     4321 tctcggcgcc aatcttcgcg gcgaaacgtt ccgcaatgcg cggggcaagt ttctcgaata
     4381 tttgtttttc agccaaatcc ttgccgtaat cgatgcccga ggcagccgcg gccgctttcc
     4441 acatttcaat cctttggtcc cggccgtgag cttcaaatcc gtagagcaaa gcgaggcgtt
     4501 ggattagccg cagcgtgatc accgtgagca agctggcatc cggcaaaaac gtgatcatgc
     4561 cgccaaggcc gaatcccgcg ccttccgcaa gcgccagccg ctcggcatcg cgaatcagcg
     4621 tcttagcaat ggcgtccaga cgctccgtgg gaacgtcttt catccgcgca aagtgcggaa
     4681 cccacaagcc gtgcttgttg gcgaggtggc gccggaagtc ttctgggtcg atttcgatcg
     4741 aacgcagtcc gcggagaaca ccaaaccgca gaaagcggct gaaggtacgt cccgccagcg
     4801 accccttgac cgagttatgt aaccctgcca tgcctacaat catcctaagg cggttagaac
     4861 cgtctggcaa ccgccgcgtt gcacgaactg tgcccgtggc gccggactgg tgaatcccgg
     4921 ggaaaggggg gagttgccag aaacgcggca agaaaacggc agtcctactt gacgcggaga
     4981 cgcttttcgg taaactgcat tcttcgctat ggccacgatg ccacaattcg ttccgtccga
     5041 agtcgtgcat gatctcgact tcccgcagcg cgaagccgcc tttttctacg gcctgttctt
     5101 gcgcggttat tccgcggacc agttgcggcg agacatcgaa gtgccccccg tggtccttgc
     5161 caaatggcac cgcgaggcgg aacgcgaccc gcagcttcgt gatattttta cgcgcatggt
     5221 ggattaccgc cgtcacgtcc tggccatctt cgataccctt gtgggttcgg acggtaaccc
     5281 gcagcgcgtt cagtagtctc tccattttcc gtgctgcttg cgggcagggt tgccgatctg
     5341 gttctgggct gtgtcccttc ttctccgttg caattcccct gtaccttcgt tcaggtcaaa
     5401 aactgtcagg caaccaaagc ggattgccag tgatctaacc gatttggggg gacaagtgtc
     5461 acaaaatgaa ggagattcct ctgattgacg gggaactagt ctggggttag aatcggaacg
     5521 tcctaaagct tgaggtgtct tcatgtcaag ggtagtacgg accctgttcg tctctctttt
     5581 tttgatggcc atcgctattc catcggtttc cgcccaacag gcgcaggatc cggggacccc
     5641 tccagcaccg caaaagatgg ataaagaagc aaagcggaag atgcggaaga cgctgaagga
     5701 gttgggcttt gagtaccggc aatggttgac ggaagacttc acctacatca tctctccaga
     5761 cgagcgcaac gctttccttc aactggatac caacgaagag agagaacagt tcatcgagca
     5821 gttctggctg cggcgcagca gcaatcccga cctgccggag aacgacttta aagaagaaca
     5881 ttaccgacgc attgcctacg ccaacgaaca ctatgcttcg ggaatcccgg gatggaaaac
     5941 cgatcgtggc cgcatgtaca tcatgtgggg cccggcggat gaggttgaat cgcaccccac
     6001 aggcggcacg tacgaccggc cgatggatga gggcggaggg tccacgacca catatccgtg
     6061 ggaaacatgg cggtggcgct acctggaagg catcggcgag aacattattt tggaattcgt
     6121 cgatcccagc ggctcgggtg agtatcgcct gaccatggac ccatcggaaa aagacgcgct
     6181 cctgcacgtg cctggtgcgg gtctgagttt gatggaatcc atgggcatgg cgtcgaaggc
     6241 cgaccgcttc acgcgtgccg acggcaccaa tctgccgagg agcatgggtg gtcaaccggc
     6301 cagcatgaac gagttcagcc ggctggaact ctacgccaag gtcaataaac cgcctgaagt
     6361 gaaattcaag gatctcgaag cggtagtgac ctcccgcatc gtccgcgatc aagtgcattt
     6421 caactggcgg accgattacc tgaaggtgac gaatgacacg gtgctagtgc cagttaccgt
     6481 gcaggtcccc aatagccagc tttcctttca ggccaaggat ggaattcact ccgcggtgct
     6541 gaatattttc gggcgcgtga gcacgctgac cggccgcgtg gtgcaaacgt ttgaagattc
     6601 cgtcacccgt gattttccgg attctcttta ccagcaatcc ttgaaactcc aatcgatcta
     6661 tcagaaggcc gttcctctgc gcccgggtct gtaccgcctg gatctggtga tcaaggatgt
     6721 gcagagcgga aatatcggag ttgtgaactc gcgcctgcag gtgccgcggt acgacgatga
     6781 caagctagag gccagctcct tgattcttgc cgatcaaatc gagcatgttc cggcgaagca
     6841 aatcggctcg ggacagttcg tgctgggttc ctcgaaggtg cggccgcgtc ttggaggtga
     6901 tttcacgacc gccgacaagc tcggcattta catgcaggtt tataacttga agcccgacga
     6961 caagacgcat aagtccagcg ggactttcca gttcaccgta aagaagggcg accaaattgt
     7021 accgggcatg caattcatta taacgacgga agagatgaag cagacgggtg atcaggtcac
     7081 catcgagcgc cttctgccgc tcgcaacgct gccaccgggg aagtacaaga ttgaagtgaa
     7141 tgctacggat acgctttcga atcagaccat ttcgcgctcg gcagagttta cggtgaaagc
     7201 gccgccggaa accaagaccg cggcaaatgc agcaccgggg aggtaaagca aagtgaaaca
     7261 ggtacggctc cgaatcctgg gggccctggt cctgaccgtc atggtttcgt ccttcgcggc
     7321 gattccggcg gccgcgcaag cgaagcctgt cccgggcaag cttgcaggag tagtgcgcga
     7381 cgctgcgggt acgccgcaga tgggcgcgag cgtggagttg atgtccgaag cggtgggggt
     7441 ggcagccagc cgtggcttcc tgacgaatac gcaaggcatg ttccggggcg acaagctcgc
     7501 tcccggtttt tacaccgtcc gcgtgactct cgccggtttt cttccaacac ttgagaaaca
     7561 catccgcgtc acttcgaatc tgacgaccgt cgtgcgcatt gagctggaat ccatgtttgc
     7621 gtcgctggat cagttgcggc gcatgccctc gaacagcacc gcggaagttg acgattggaa
     7681 atgggtgttg cggtcggctt cagcgatgcg tccagttctg gaatgggtgc accacgacac
     7741 gttgagtgtc tcgaccctca ctggcgagtc cggccgggtg cgcgcgccaa gaatccggat
     7801 ggaatttacg gatggcgcgc ggcgccccag ttcggcttcc agcattgcct cttcacccgc
     7861 caccgccgtg gcctacgacc agaagttggg cggcacgagc agaatgatcc tagccggaca
     7921 ggtgagctac gacggtgatt cccccgccgg cggcatcgcc acggtgtggt tgccaacggg
     7981 agcgctcggg gctggtccgc ataccgcgct ggtccttcgc gaagccaagc tgggacccgt
     8041 gggtccgacg tttcgaggta tccgttttga tcagggtggc gtgttgacac tcggagatcg
     8101 cacggttctg cggtatggcg gcgaatatgt cttggtggga ctgggagccg cagcctcttc
     8161 gctccgcccg cgcgcggaat tgaatgtgcg cctcaccgat gattggagca ccgccatcgt
     8221 tttcgcctcc atgccgaacg gacccggccc actcgaagcg agtgatgcac agcccggtgg
     8281 cgcgctcgcg gcggcgctca atgaacttga tgcatttccc gcgctgctct ggcggcaggg
     8341 ccgtcccgtt ttgcagagcg gtctgcacga agaaatttcg gcggaacgaa agatcagtac
     8401 ccgcggaaag cttcaaatcg ccggcttcca cgatgacaac cgccacgtgg ctgtcttcgg
     8461 ccggggcagc gatctgccca ctgccgatta ttttcaagat tatttttcca ccggctttgc
     8521 ttacgacggc ggttcctcca gcagttgggg aacgcgcgtc gcatttcgcg agaagctcga
     8581 cggcgacgtg gaattgacag ccgtttattc tttcggtgga gctttgacgc cttccgacga
     8641 cgccgacggt ctgcttcgcg atgcgcttcg cacggcacag cggcattccc tgggggcggg
     8701 aatttccgcg aaggtgcccc gcatcggcac gaaagtccag gcgggatata aatgggtgag
     8761 cggcatcacc gtctcctccg tagacagcta tggcgagtcc ctcttccaaa tggaccccta
     8821 cctacacctg attgtccgcc agccgctgcc aaagtttgtg ttggggcggt gggaggccat
     8881 tgcagattgc aacaatctct tggcgcaggg ctatgtctcc atcaatagca gggatggccg
     8941 agttgttctg gtacctgcat tccggacatt tcgaggcggg ctgagcgttc agttttagga
     9001 atttctgggt tctcgaaagc tgtatttgtc cttccagaaa ctggatgatg cgggatagtc
     9061 cagcctttcg ctacggtaaa ttattgaata caaaagagtt tcgcggatga gtatctacca
     9121 aacaatggac attgggttgc acttggtttc ttgtgatggg gtgtagtcct gccccgtaga
     9181 gttatgaggc aaaacctccg agttagcctt ggggcggtcg tgctggcgct agccacgctg
     9241 gctgcggtga tttttgccct gattaacttc gaccagcgca gccgctttga ggtggtgtac
     9301 gacggagtgg cctggctcga tacggaccac ggcattcagg ccagccgggt ttcccctaat
     9361 tctcccgcaa ctcgcgccgg gatccggccg ggcgacgtcc tgctgacgat taatagcgcc
     9421 tcggtcacaa gagccgcgga ggtcgctcgg cggctggacc gtgctgggct ctggacccag
     9481 gtgcgctaca agctttcccg cgccggtgag gaattcgaaa cgccaatcct cacagcgcct
     9541 gccgaaaagc ccctcgcgac cgagaactac ctgcgaatcg taggcttgct ctacctgttc
     9601 atcggcctgt tcattttcat ccgccgctgg aacgctccac gcgccgtaca cttttacgtc
     9661 ttttgcttgg tctcctttat tctctggtcg ttccatttca gcggaaagtt ggacaccttc
     9721 gactgggaag tttactggtc ggagattgta gcgcgactgc tggcacctgc tttgcttctc
     9781 cactttgcat tggtatttcc ggggcggtcg gaaacaacca ttcgttcgtc ttcgaaactg
     9841 ctggctgtct actccttgcc cgtcgcgctg cttctgattc atttaagcac ggccctgaac
     9901 gccctgggtt ttgtgccctg gctgggtgcg tacctgctgc tcaggaaaat cgaatttagt
     9961 tatcttgccc tttgcttcct ggcagcgggg cttgttttct accggagcta ccgcgaggcg
    10021 ccctccggtg tccttcgcca acaattgaaa tggctcaccg gtggaactct tgccggcagt
    10081 ctgcccgttt ccttgctcta catcgttcct ttggtgtccg gcgtggcctt gcgcccgtgg
    10141 atgcagctct ccgtactaag cctggtgctg atcccgctgt gtttcggcta cgccgtcatc
    10201 cgctaccgct tgatggatgt ggacatcatc ttcaagcgtg gcttggcgta tacggcggca
    10261 accgccgggg tggctgcggt ttactttgcg ttggtcgcgc tgatcgctgg gattttccat
    10321 gcgcccacga gcggtcccgt cggcggcatg atcgccatcg tcatcgccgc gtttttgttc
    10381 cagccgttac gcgcagggat tcaggcgcgg ctggatcgct tcttctaccg ggatcgtctt
    10441 gattaccgcc gcacgctcat cgagttcggg cgcacgctga ccaacgaagt gcgtttcgac
    10501 cccatgctgg gctcggtgat ggaccgtatt tcccaaacgc tgttggtcga ccgtctcgcg
    10561 atttttgtgg aaagcggcct gcagcccggg cagatgcgca ttgcccgttc gatgggcgtg
    10621 cggctctccg agtctttgga tcttagtttc ctcgagccgg cccggcccga gtttgcccgc
    10681 ggggcgctct tttttgagtc gccccgtgcg gcaagagacg tgagtgactc cgttcggcgc
    10741 acgcttgagc agctcgacct caattacttt gtcccctgcc gcattcgcga gcacaccgtt
    10801 gcggttctcg gcctcggcaa aacggttgac ggagattttc tgtcgagcga tgacgttgag
    10861 ctggtcgaga cgattgccgg ctacgtcgct gtagccctcg acaacgcgca gctctacagc
    10921 tcgctcgaac agaaagccct cgaaatcgcc cgtctcaagg atttcagcga aaatatcgtt
    10981 gaatccttga acgtcggcgt gctttccatt gacctggagg gcatcgtcga atcctggaat
    11041 acgcgcatgg aacagctttt cggggtggtg cgccaggaag ccgtcgggcg ccagctcagc
    11101 tcgctgttgc ccgaagaact ggcgcgcgaa atcgccgcgc gcggcgacca ggaacaaatt
    11161 accggcatct ataaacagag actccagcac cagggcaaat tgctgaccat gaacgtttcc
    11221 atcactcccc tggtgagcaa atcgaatgag cgcatcggcc gtctcctgct cttcgatgac
    11281 gtcacccagc gggagcgcat ggaagagcag atgtcgcaga cggagaagct gacgtctctt
    11341 ggactactcg cggctggcgt tgcgcacgaa gtgaataccc cgctcgcggt catctccaac
    11401 tacattcaga tgctcgcgaa acagatgccg gaaggcgacc cgcgccagtc aatcattgaa
    11461 aagatcgtca agcagacgtt ccgcgccagc gaaatcgtca acaatctgct caatttttcg
    11521 cggaccggcg cggcggaagc cgcggacatc gatgtgaacc gtgtcgtcga agaaaccttg
    11581 tcgcttgtgg cgcaccccct caaaagctcg cacatccagg tggtgaagca actcggcgaa
    11641 ccgttgccgg ccgttcgtgg atcagccaac aaactgcagc aggttttctt gaaccttttc
    11701 ttgaacgcgc gtgatgccat gcccagcggg ggaatgctcg aagtccgcac cgccgcgcac
    11761 aacggcagcg tcgaaatcga agtggcggat acgggcgccg gcataccccg cgaacacatc
    11821 catcgcatct ttgacccgtt cttcaccacc aaagccagtg gccgcggcac gggcctcggg
    11881 ctttccgtga gctacggaat catcaaggaa cacgccggga agattgacgt acgctccacg
    11941 cccggcaaag gcacctcctt ccacgtcgag ttcccggcag tcaggaaagc ggtccatgtc
    12001 taagggttcc atccttgtgg tcgacgacga atcggaaatc cgcgaaggcc tcgaactcct
    12061 gctgagaggc gaaggctacg gcgtctccag cgcggaaacg ggcgaatccg gcctcgagaa
    12121 gctggaacag catccttacg accttctcct gctggacgtc agcttgccgg accgcaacgg
    12181 tctcgacatg ctcaaggaga ttcaccgccg cgatccgcag ctctccgtgg tcctgattac
    12241 cgcctacggc tcgatcgata tggcccgcgc cgctttcaag aatggcgcga tggactacat
    12301 caccaagccg tggtccaacg atgaattgct tgcccaggtg gcgcaggccg tggaatcgcg
    12361 ccggcttcgc gatgaaaacg tacagcttaa acgcgccctg aagcagcgct tcaatttccc
    12421 gaacattatc ggcaaaagcg acaaaatgca ggtgctgctg aacctcgtca cgcaggtcgc
    12481 gccttcgcgc tccacgattc tcatcagtgg tgaaagcggt acgggtaagg aactcatcgc
    12541 caaggcggtt cactccgcct ccacccgcgc ggacaaagca tttgtgcccg taaatacggg
    12601 atcgatcccc gtggatctcc tggagtccca gcttttcggc catgtgaagg gcgccttcac
    12661 cagcgccgtt gcttccaaga agggcctctt cgaagtcgct gatcaaggca cgattttctt
    12721 cgatgaaatc gccaccatca gtcctgaaac gcaagccaag cttctgcgcg tgattcagga
    12781 gcgcgaattc atgcgtctcg gcggcacgga gcaacttaaa gtcgacgtgc gcatcgtagc
    12841 cgcttccaac gtcgaccttc ttacgctcgt gcgcgaaggc cgcttccgcg aagatttgta
    12901 ccaccgcctg aacgtgatcc acctccagat tccgccgctg cgcgaccgcc gggaagacgt
    12961 gccccttctg ctggcgcatt tcctggagcg gtattgccag gaaaacaaca agccactccg
    13021 gcagtttacg caggtggcga tgaagctgat gatggattac gaatggcccg gcaacgtgcg
    13081 cgaactggaa aacgtcgtcg agcgcgcggt cgtcctctcc acgcaggagc gcgttgacgc
    13141 ggacctgctc ccggaaagca ttcgcagcaa ggaaatcgtt cgcggtgtgc gcctgcagct
    13201 ctcggaattt ttgccgccgc ttccgggcga acccggttcg cgctcctccg cggacaatcc
    13261 gcaaccgtcg ttgttccaga tcatggacga aatcgagcgc cgcatcatta ttgatatgct
    13321 ggagcgcacc ggctggaacc agaccgaagc ctccgagcgt tttctgatcc ccctttcaac
    13381 gctcaaccag aaaatcaagc ggcttggaat tgatgtccgc cgccgcggcc gcagtgatga
    13441 gacgttgtcc gcctcgatag gaaaatagcc gccggatggc ctccggaaat ctctccttga
    13501 tgaactgccc cgcgaaatag cctgcccaat cacgtaaact acgcttggaa cgctatggcc
    13561 gagtccacat ctccatttga tccagccgat accacttcct tcgcgaacga atggctcgca
    13621 ctgctcgagt gcgcttcttc agcgcacgat cgcgaacggc ttgcaggttt gctccgttcc
    13681 gcggattggg cgcgcttgct tgtcctcgcg gaagagcacg gcgtggccgg ccatctggca
    13741 gcgtgcctgc gcggtctcga agaggacttg gtcccgctgg aaataaaaca aacgcttatc
    13801 gatcgccagc gcgcgcaggc attttttact ctacggctga ccgcggagct ctttcgcata
    13861 ctggaccgct tcacctcgga gggaatcggc gccctggtgg taaaagggcc ggtgctggcg
    13921 atgcaagcct atggcgaccc ggcgatgcgc agctatggag acctggatct gcttgttcgc
    13981 cagcgggaca ttcgccgggc cacggaattg atgagcgctg caggctttgc ggccgcggtt
    14041 ccgcttagcg ccatcgacgc cggaaaaatc cccggccaat atcttttctc caagccggat
    14101 ttgaaactca tcgtcgagct gcacaacgat ctcaccttgc gctattttcc ccgccggctc
    14161 cccctcgagg aatttttcgc gcggcaaatc cgcgtgcgca tcgatgcccg tgaagctctg
    14221 gccctttcgg tggaagacga actggtgctg atttgcatcc acggggcaaa acatttttgg
    14281 gagcgtttga tgtggattgc cgacgtcgct gcgctggttt cgcgccaaac gggtatcgat
    14341 tgggagcgcg ttgcggattc ggcgcgcgcg gtgggcgcgg agcgcatgct gcgcaccggg
    14401 ctgctcctcg ccggagactt gctcaaggcg caactgccgg agagagctca agtgatggtg
    14461 caggccgatg tggtggcggc gcggctcgcg gaacaaattt gcacgtggct gcccgccgct
    14521 gggtattcgc cgcctgggct ctttgagcgt gccgcgtttc gattgcgcat gcgcggcaat
    14581 ctgatcgctg cccctgccta tcttctcaga ctttcttttt cgccaaccga agaggattgg
    14641 aaggaaggcg gggaaggaag tccgcacaaa ttcctcgaca tggttcgccg gccgttccgg
    14701 ctcgcgcgca agtatggccg cgacaacaag agttgatccc cggagttcca cggtcgccgc
    14761 aaacgaactt ttcagcccgc acgggcttca gaaaagggcc gcgcgtgatg tctccatcac
    14821 gcgcggccct gtgtccggat tccagccttt agttaactga tatttgcacc tggactgtat
    14881 gcgtcaatgc gccggaagtg cccgtaatcg tcaaagtggc cgcgcccttc ggcgtatgcg
    14941 gctttgccgg gcccgagcac cctgccagga cgacgaataa catcatcgcc gtgaccatcg
    15001 cgaaccgcgt gcgcaactgt tgagtcctcg gcaagaggaa caggagcacc agcgccagaa
    15061 ttagcggaac gacctgccgt atcgacatcg gcagagtcgg caccctcggt ggcggaacaa
    15121 ccattgccgt cgtcgtcatg gacacttgcg cctgaaccga cgatggcggg gctaccggtg
    15181 gtgtgactgg tgatgacgga atcgtgcacg tagccagcgc cggcgctccc gtgcaggcca
    15241 aggctacagg agagctgaat ccgccgactc ccgttaccgt cacgttgaag ttgagcgtac
    15301 tgccgtcggt cacgttttgt acggaggtcg gaccggtcaa tccgaaatcc ggcggggtgt
    15361 tcgtgccact gccggtcagg gaaatcaatt gcgggctggt gctgtcgctg tctgtcagtt
    15421 gcaatgtggc cgtttcagct cccaccactg tgggcttgaa cgtcacactc acagtgcagg
    15481 tattggtagc gccaaagggg atgctgccgc tgcacgagtt ggttaccttg tagtccgtat
    15541 tggctgctgc cggattcccg ttcaccacgg cgctggcaaa cgcgatcgcc gcgttcgtgt
    15601 tattcgtcag cgttaccgtc tgcgccggac tggtgttagg aataaggatc gtcccaaaat
    15661 cgaggctcgt cggtgccacc gtcaaagtcg cgatgggggt cagcttggcc acgaagccat
    15721 cggaagtccc attcagggtc gtctgaaacg ccgttggggc ggtctttggg aaaacaggaa
    15781 aagtcgccgc cgtggaatag gtttgccccg tcacataggc gttagcgggg tttgatgtgt
    15841 caatggcgat cgcgaagata tgatctccat tgggagcatt tccgtttcca ccaaagtatg
    15901 tcgaataaag caggtcagcc gcgttgttac ctgcgggact gagcttggca atgaagccca
    15961 cacttggggc gccggggggg taagtcggtt cgaagccgcc tacgatcatc cctttcggtg
    16021 gaaaaggaaa attcagtgaa gaggtcgttc ccgcaacgta agcgttaccc tgggcgtctg
    16081 cacgaattcc aaaacccgtg tcgactcccg ttcctccaag aaaggtcgaa tatttcagcg
    16141 aaggaaccgc agcctgtgta gtatctatca gacttacaaa agcttttcca cccgctgctc
    16201 cgctggtatc gaacgctcca ggttttgggg taactgggaa atcattcgag ttcgtagttc
    16261 ccgtcacgta agcaacgttg cccggcccaa gtgagatggc gtaggccaaa tcaaaggtcg
    16321 cgccacccaa ataggtcgaa taattttttg aaaggggccc aatctttgtc gtgtcgattt
    16381 gtgtcacgaa tgccgtcgcc ttggtattcc ctgcgggacg attgggctgg tacgcattcc
    16441 cgaacgtcgg aaagtcggtc gaggaagttt ggcccgcgag gtaggcgttg ccggacgcac
    16501 ctatggctac gccaaatgct tcttccccga atcccaatcc tcccgccacc aacgcatttg
    16561 ccccggcgcc gcccacgtaa gtggaataaa tcaaggaagc gcttccggat ttggtggtgt
    16621 caattttggc gaggaaagcg tttccatttg tggagttcag agtcgtctga aagccgttca
    16681 caacaggaaa gtttggcatg gatgtcaccg tagtggaggc cgtcgagtct gtatatccgg
    16741 ctacgtaggc gatcccattc gcatctaccg caatcgcctc cccaaaatct cctccaaaca
    16801 aagctgttcc gttggtgcca ccaatgtaag aagaatatag caacgaggcc gcgccactga
    16861 cggtcggatc aagttttgca atgtagctgg ttccgtttac gttgccagga ttgcttccac
    16921 tcttgaagcc atttggcgta gttggcatgg ggaagtcagt cgcaaacgtg cctcccgtga
    16981 cgtaaatctt cccaccggaa tccacggcaa tggcgttggc gctttcgaac ctattagagg
    17041 ctgtgccggc caaatacgta gagtagagca actttgttcc tgtggggtcc aactccgcga
    17101 caaacgaagc tcccaaattg ggtgttgggc cagtaccgaa ggccaccttc gtcccggcgg
    17161 ggaagtctgt ggacattgtt tcacctgcga taaaagcgtt gcccgaaccg tccaccgcga
    17221 tcgcaaagcc tacatcaccg gcgctcccgc cgaggtacgt cgagtagttc aacaccgggt
    17281 caaggatcaa tggctcggtg cggtcgtagg aaccgaccgc gaacgtgacg cggcgattcc
    17341 tgtcgatcgc atacccgcca gcaatctccc gccgttcccc cttcacattc tggtagacca
    17401 ccggtttctg tagctccact tcaccgcccg caacgctcag caccagatcg ccgcgcgagt
    17461 tgacgcacat cttgcgcgcg ccgtccactt tcatggcgat aaccttcggg tcggctcccg
    17521 gcgccactac gaagtcatat tccagccgcc gctgattgcc atagaaaacc agatccactc
    17581 cgggatagac tcctgcatat ttcacgcgcg cataagaggg cacgtcggtg tgccagttct
    17641 ttggatcgtt gccgatgaaa tagttcgtct ttcccggcag ttggtccatc cccgcgatct
    17701 tcgcatcggg attcgcccct tccagctgca tgcgaattgc tgttgactgc ccagcgcgtc
    17761 gcgctttgcg gagcgcccga agcgtggccg tacggcgcag tggtgacagg tcgcgtggga
    17821 cctttgatcg caacgcaagt acagcttcct gcgatgtcag aaagagttcg tagccgctgc
    17881 catgagagac gtatcgcact tcgcgagccg tctggccctg gttttcttca aagctcagcg
    17941 gcaacttgcc ataggcttcg ctccacttgg gatcaccttt cgtggcggag attttggatg
    18001 cttggctcgc cgcgacctgt gaccgtcccg ctccagtttg cggagagcgc ttcctcgcgc
    18061 ttcgaaaagc cgcaaccatc aaaagaccgc aaacaagtcc ggtcacgcct acgaacttcg
    18121 caactttcac ccaagaaccc cgagtcttca ttttggtaat cctcccaagt cttttcttcg
    18181 ctgctcatcc ccaccgccag ccgcctctga accagccctt gatccggcct gaccctccag
    18241 acattcccaa tttggaacga gtcacgctct tcgagtcaca gttgttctga aattttctgg
    18301 agagggagca atcatgccgc acgccgttaa catagtgccc caaatcggga aggtccactc
    18361 tgaatttaga atcgcaactg tatcgtatca gataactcgt aggcttccag tgccgaattg
    18421 ggaagaactg cccgggcagt cagagccgcg cacttgtcgg gctacgcagc gacgcaaaat
    18481 cctcgcggat caccgcacaa agctcttcaa gccgggaggc gtcgttatga gggtggatcc
    18541 gccggatcgg aaccgtcgtc accagccggc caagaaccgc aaactcgcgc gcccgcatct
    18601 cccggtcgag aatcttgttt gcgaaggtgt cggctacaag ggaaagaagc ccagcctgtg
    18661 gccggacccc ctccacgaat ggagcctgat cggggcggcg gtcgcccagg agatagatgg
    18721 cgccgagcgg aagcgagcgg ttctcgaacc ttgttccctg gtctcccaag gccagcctgc
    18781 gcttctccca tcccgtgctg aagcgcggga gcgcatcggg tgaaccatac agcatctcca
    18841 ccgaatccgg ccagaggcat aggtggggat atgccggcat gacgcggaag ccctccgggc
    18901 tttccaccag cgcggctaca tcatcagaaa tgacgccaag ccctgccggg cgaacgcggc
    18961 cgcggtggtc gactttcccg ccccttccga gccaacgaac gcaacacaac gtttctcaat
    19021 ggagacggca cttgcatgaa gacaggtcac gcctcgcagc cgcagcaaaa gtcccagcac
    19081 cggtcccagc agataggaag cggtatcttc gagattcgat gcttcgggcc aaaccgccca
    19141 cagggacttt ccctctcgtt ccagccagaa ttgaacgcca tcgtaatacg ccaggcggag
    19201 aaacccgccg tctgcggtct tccagattcg caaggccgga ttgccggatt cgtcggtgta
    19261 agagcttgtg taggtaagga cttccgaatc ggctggaatc tcgcttttcg aattaggcga
    19321 aacgcccaaa tggagttcga catctgcggc gtcggaagga ctccccaaag gggccagtcc
    19381 tggaacggga agattggatc ggactttcag gccgaaaacg gtatgagtgg aatccacttc
    19441 gttcgacaat ctgaatgctg gggtctaagg gccagatctc gatcctgccc gcactatttc
    19501 tttctcttct tttgctcgct ggcacctgaa tcttcagatg ataaatcaga agcgttccgg
    19561 tccgtttaca ctcttaccct ggtgctgcct caccttcgcg ctagacgtga gtccgcggtg
    19621 taagtctgcc tccgccatcg ctttggcctc ggactccctg gctttgcgtg agttcccgca
    19681 ccgtcccata aacggtcaac gtcggcttcg aatacggctt tttcgtctcc cgtttgacgg
    19741 acttaaccat taccggcctc cgcatatatg ttatatcgaa ttcttcgtgc cgactgcaac
    19801 caaatgttga ggcagtaggg ccgtaagtga gtacgtatct gctcggcgtt catcttacca
    19861 tgcggcgcgg ccagcgccga gcattcgata aacctatcgg aatctttgct ccactgcatc
    19921 tgattcaatc tctcggcccc ggttttctgc agttgggccg agaccggatc gccctgcaac
    19981 ggcgttttcg ggcgcattcg aacgcgttcg gggatgcgct cggccatcgc ttcccgcaga
    20041 agcatttttt cgaagaacca cgggaagggt gcaatcgcca gaagataatt cacgatccgc
    20101 aagtccagga acggataccg tacctccagg ggatagggcg tccatccggc gttctcctgt
    20161 tcgaacatat gtgtccactg tggcagggaa agagaagcgt gggcgttcgg aacaatagga
    20221 tgcctgctgg gcatcacggg atgctcgcac agttcttgcc atcgcgcctt gagctttgca
    20281 cgatgcagga attctcggtt gagccacggc ggaaaaaccg gcatgtccgg atccttgccg
    20341 atgattctct tcatccgcgt gcgaatgccg cgccacggaa aaggtctaat ccaaaaatag
    20401 tttgccatct ccgtcaaaag acgccgccat tccccgcttc gccgcaggtc agccgcgtac
    20461 ggccacattt gaaattccag caggttgtcg acgccttccc cggacaatag aacccggcaa
    20521 tccgcggaaa tgttccggca ggagtcaaga aaacccgaaa acagcggatc ttccaccggt
    20581 tccggcaaac tgaattcagg attatcccag ccttcaaaaa gctgcgtttg atccgaggcc
    20641 atgaatttaa ccgggaggtg caaaaactcg cctacttcgc gcgcgtactc gccttcccgg
    20701 tcagggatca acgattcgaa cacatgcgtg tagccgcgta tgtccgtggt ttgggcagac
    20761 tttgctgaca cttctttcgc taccgccgcc acggccgaag aatccacccc gccgctcaag
    20821 agaattccaa cccggtccgt gcgcaagcga tccgcgaccg cggcttgcag gagcgactgg
    20881 aaattctcga cgtactcttc gggtttcgaa tagcgaatcc gcccgtccgt cggcggcgtc
    20941 cagtagcgtc tgagcttcaa accttccggc gagatactca gggaatgcgc ggggggcagg
    21001 cgttgaatat cgcgaaagct ggtcgtggca ttgtcataat tcagcccaaa cagcagaaaa
    21061 tccccaatcg ccgtttcatt tagttcgcct gacacctccg ggtgcattcg aatgcaattc
    21121 agcgtgttgc tgaaaagaaa aagatcctcc tgttggatgt aataaaatgg cttgataccg
    21181 aaatgatcgc gcgcgcaaaa caattgtttg ttgcgcgcat cccagatggc gaacgaaaaa
    21241 tcgcccctta ggtgctccac gcacggcgtt ccccaagttg cataagcatg cagaatgagt
    21301 tcagagtcag gagcgtttag ccgaaccacg cggccggagc gttgcagctc ggcgatgaat
    21361 tccgcccggc cgtcgagtcg cgcgtccgcg gcaatccaaa accgcccatc gagactcgca
    21421 ggctggcgtt cgccgagcga ttcgcgcgtg gtccgcagca tcgcatgacc cagtccgatc
    21481 gaaccatcca tccagcactc tcgggaatct gggccacgat aagagagaaa atcgacaagg
    21541 gactgcaaaa gcgcccgctc catcggcgca ccgttccggt ggtagatgcc gacgatgccg
    21601 ctcaatcaga ttgcctttct attcgagtgg ctggcgcttc gagtgccgaa aaggaatagc
    21661 cctgccgtca ttttcgtccg agaaaacggc atcccattcc gcccgggcat acgccctgaa
    21721 gcggagttct tcaacaacga gcattccatt tctaaacgct gcttggctgg aactctattg
    21781 taccgtcaac tctctcccgc ggcaaccgca ctaggttccg tgtcatgttc cgtggatttc
    21841 tctcgcaatc agcccgagag ctcccgaaat tatggagaag cgcggttttc ccgcgacgcc
    21901 atcgtctttt gtcttgcaac tccgaaacgg tctaggcaca gtaccggaga atgattctgc
    21961 gtctctacag caacgcgcag cgcgcagtac ttgtttctgc ttctttcgtt gtcgctttac
    22021 tcctctctta tttcagcatc cgaaatgcgc ttgctaccca ctacgctggt ttgcaaacgg
    22081 ctgaagccta tgagcgcgcc gcgctcctgg agcccgctaa cccgttcaac tggtatctcc
    22141 tgggccgcta ttggcagtac aacctcgagg atcccaacgc cgcgcgcgcc attcgttcct
    22201 acctttctgc tctgtccttg aatccggcat cgtcggaaac ctggctggat cttgccaccg
    22261 cttacgaatc ggaagccaat ccggtcgctg cgcgtgacgc ttttctgcac gccaagaagg
    22321 tctatcctct ttccgccgac gtgtcttggc gatacggcaa tttcctgttg cgccagggag
    22381 agcgtgagcc ggccttcacc gaaatgcgcc ttgccgtcgt agcagacccc aagcgcgccg
    22441 cggaggcgtt ctcccgctcc ctccgcgccg gatccagcat cgagaccact ctcgatcgcg
    22501 tcctgccacc cattagcgag gcctaccttg atgtgatttg ggatcaaacg acggacgggc
    22561 acacggggaa tgcccttaaa gtatgggacc ggctcgcctc ccttcatccc cgcttttcct
    22621 tgtacgattc tttctccctt gtcggtgctt tgatgacgga aaaacgcatc gcggaagcca
    22681 gccgggtctg ggaccaggcg gtcctttttg cgggcctcag tgatctccaa ggtccgccag
    22741 gctcggtttt gtgggacggc ggattcgaat caaatgttac cgccggcggt ttttcctggc
    22801 tccattcgga gggattccgc ggcgtgcaaa ttggcatgga tacccaggag aaacactccg
    22861 gcaatcgttc cttgcgactg atatttgatg gaaagtccaa catcaatttc acgggcgtct
    22921 gccactacgt tcccgctcgg ccttccaccg cctatcgttt ctccgcgtgg gtcaggacca
    22981 gagcgctcac ctcagaccag ggcatccgct tccagttgca ttcactcgga acgcaggaca
    23041 cttccactgc tgtcacatcg gaagtccacg gaactgcgcc ttggacccgt atcgaattgc
    23101 cctggtcctc gggtaaggat gttcaggaat tgcaagtttg cctggtccgt tttcccagcc
    23161 aggaagccga gaacaagatt cagggaacgg cctgggtgga tgatgtcgcg ttagttccgg
    23221 aacccgcgga gcatcccaaa ccatgaggtt gctgcgcttc ggcatctgcg ctctggtcgt
    23281 gtttgccgtt gccgcgcacg gaggtgtcga gg
//
LOCUS       DASYVO010000482         9828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_251197, whole genome shotgun sequence.
ACCESSION   DASYVO010000482 DASYVO010000000
VERSION     DASYVO010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9828
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251197"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..1054)
                     /locus_tag="VGR03_16590"
     CDS             complement(206..1054)
                     /locus_tag="VGR03_16590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1995953.1"
                     /translation="METLFFQNASVRLHAVAAGPPDGRVVLLLHGFPEFWYGWRRQIE
                     PLAAAGFRVIVTDQCGYNLSSKPSGVAAYVLTELVSDVIAIADQLGQEKIFLAGHDWG
                     AAVAWSTALLHPQRIAKLVVLNVPHPSVMRKFLSTHPRQFLRSWYMFFFQLPWLPEAL
                     FSAFNFRVGSRSLLRSSRPGTFSAEDLAQYRSAWSQPGALTGVINWYRALFRTRVKFQ
                     DKTVRVPTRILWGERDDFLLTEMAHESLRYCTSAELFTFANATHWLQHEEPARVSQLL
                     IDFFRS"
     gene            complement(1153..1620)
                     /locus_tag="VGR03_16595"
     CDS             complement(1153..1620)
                     /locus_tag="VGR03_16595"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV1995954.1"
                     /translation="MQKTLSSFNPHGARIGFLFLPVFAAALLVASQGWSQEKTIKKTP
                     IQQSNSTSGNQMFADYCAPCHGLSAKGDGPAASALKTPPVDLTQLAKKNNGKFPMDHV
                     MNDLRQGSPMASHGSTDMPVWGPLFKSLDPTNPVMVDQRIRNLAVYIESLQAK"
     gene            complement(1786..3423)
                     /locus_tag="VGR03_16600"
     CDS             complement(1786..3423)
                     /locus_tag="VGR03_16600"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HEV1995955.1"
                     /translation="MHTTLLLTVSLAAQNPISHYWHKITDPTFRGMYHANAFDLAMMI
                     PYFIVLLVLAVYGLHRYWLVYDYFAYSKNVPATPPPVTNWPRVTIQLPIFNERYVIER
                     LVEAVSRFDYPPELLDVQVLDDSTDETCEVARACVERHGAQGIPIVYIHRSNREGYKA
                     GALENGLKISKGEFVAIFDADFLPEPDFLRRTIPYFMNPDGGGKIGMAQTRWTYLNSN
                     YSLLTNVETILLDGHFVVEHGARSRRGTFFNFNGTAGVWRRQAIDDAGGWEHDTLTED
                     TDLSYRSQLKGWKFLYLPQIECASELPVDMNGFKAQQARWAKGLMQTAKKILPKVLKS
                     DAPWHVKAEAFFHLTGNISYPLMILLSTMLLPAMIVRFYQGWFQMLVIDLPLFLASTC
                     SISSFYLVAQRELRPKTWRRTFLYLPFVMATGIGISVRNAQAVIEALLGKKSEFARTP
                     KFNIEGKQGTFAKKSYRNKAGWMPYAEVLLGLYFSLTIVYAISNENYATVPFLLLFVW
                     GYLYTGCMSLGQTYFARLRFGVNAPEMRPATSGAPGF"
     gene            complement(3545..4507)
                     /locus_tag="VGR03_16605"
     CDS             complement(3545..4507)
                     /locus_tag="VGR03_16605"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HEV1995956.1"
                     /translation="MKAILVREFGGPEVLKLEDVPTPKPAAGQVLVRIHAAGVNPYDT
                     YMRAGTYPVKPPLPYTPGSDAAGVIEAAGDGVKKVKPGDRVYTAKTVTGAYAEYALAL
                     EEQVHSLPEKISFGQGAAVWVPYGTAYHALHHSAEARAAETVLVHGASGGVGSAAVQM
                     ARAMGLTVFGTGGTQKGLDLAKREGAHQVFDHSKAGYQEEILKATGGHGVDIILEMLA
                     NVNLAHDTKLLASHGRVIVIGNRGEVTINARELMGRRASIRAFTLWGVTPAEETEIHA
                     GLIAGLENGTLRPVVGKELPLAEAARAHKEILEPGAAGKIVLVP"
     gene            complement(4576..5733)
                     /locus_tag="VGR03_16610"
     CDS             complement(4576..5733)
                     /locus_tag="VGR03_16610"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEV1995957.1"
                     /translation="MPHMIPAPGAFAAGVAIFLIVLWDAFEAIILPRRVTRKFRLARL
                     FYRSTWNVWKFVICLIPKPKTREALLGFYGPLSLLVLVGVWAVGLVLGFGLMQYGAGS
                     AVVMTGSQPSFLTDIYLSGTTFFTLGMGDVIPRSGLARVMVVLEAGFGFGFLAAVIGY
                     LPFIYGSFSKREVNISLLDSRAGTPPTAGELLRRHSYAHGQDALRVLLKDWELWCAEL
                     MESHLSYPVLAYFRSQHDNQSWIASLTAILDTCALVIVGVEGVCEKQAELTFAIARHA
                     VVDLSQVFGTAPKPLPQDRLSAQDLRQIRDILAQHGMKLHDGEEADRKLIGFRKMYEP
                     YIYALANYLNQTLPPWIPLKKGKDNWQTTAWAQSAGLIDKEVATVVADDHF"
     gene            complement(5811..6236)
                     /locus_tag="VGR03_16615"
     CDS             complement(5811..6236)
                     /locus_tag="VGR03_16615"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEV1995958.1"
                     /translation="MQRKASAVWQGGLKDGKGSISTDSGVLKQTQYSFSTRFENGVGT
                     NPEELLAAAHAGCFTMALSAQLGNAGMTASRLETTATISLEKLPDGFAITKSHLDLVA
                     QIPGADKAKFDAAVKAAETGCPVSKLLKAEISVTARLES"
     gene            6415..7130
                     /locus_tag="VGR03_16620"
                     /pseudo
     CDS             6415..7130
                     /locus_tag="VGR03_16620"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
     gene            7320..8351
                     /gene="pstS"
                     /locus_tag="VGR03_16625"
     CDS             7320..8351
                     /gene="pstS"
                     /locus_tag="VGR03_16625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV1995959.1"
                     /translation="MRTRSYQLLTVFLGWILLLPATTRGQEKTVLVETGSSMPEPLYK
                     NWIDEFHKHQPSTDIRYMATGTAESARNILAGSGDLGGGDAPIPEAQLRAAGRSILEL
                     PAVLIGIVVIYELPDVKGELKLTGPVLASIFLGKIKSWNDPAIAKLNPDMNLPGLPIR
                     VLHRNEGKGSNYILSDYLAKVSPEFLAAAGRSESPKWPVGQGFQRSQDLVAKLRGTPG
                     AIGYTELNLAAGSAVRMASIKNAAGEFVKPSANSIAAAATTSSSKMNDDFRVSLTNAP
                     GKESYPISSFTWFFVPAVAQDPGRGSAVAAYLKWVYTSGQKIAQEKGYAILPPEVLQK
                     VVAKAATIH"
     gene            complement(8601..8807)
                     /locus_tag="VGR03_16630"
     CDS             complement(8601..8807)
                     /locus_tag="VGR03_16630"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV1995960.1"
                     /translation="MRQVILYPGEIGYWVAECPSLPGCVSQGKTKEDAIQNIREAIEG
                     YILALQEDGLAVPPDNFDAVLVAV"
     gene            complement(8841..>9828)
                     /gene="mqnE"
                     /locus_tag="VGR03_16635"
     CDS             complement(8841..>9828)
                     /gene="mqnE"
                     /locus_tag="VGR03_16635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HEV1995961.1"
                     /translation="YFNVNRHINPTNICVAHCKLCAFGRDPSAPGAYNFSLEEIYARA
                     EQGAREGALEFHIVGGLHPDLPFEYFLEMIRGLKRRCPGVHLKAFTMVEVGYFARIAK
                     LSAKETLLKLKEAGVDSLPGGGAEIFHPRVRRIICDHKVSGQMWLNIARQAHEIGLRS
                     NATMLYGHVETEEERVDHLVKLRELQDETHGFVAFIPLAFHPDNTALSHIPKPTGYAD
                     LRNIAVSRLLLDNFDHIKAYWIMLSPSIAQIALRFGANDLDGTVVEEKIYHDAGAKTS
                     EFTPRNELERLIRAAGRVPVERDTLYHPVDRSKMPPLPPPTRADVVSLTLNV"
BASE COUNT         2066 a         2685 c         3079 g         1998 t
ORIGIN      
        1 ggtatcgcga caagcagagc ctcaatcgaa ccgtgccaaa ctactacgac gtcagccgcg
       61 tggccttcgg agcggcccgc tacggcggac cagggcagta accagcactc tcccaaggcg
      121 gactcttggg gcgagcgaag agctcgctcc gggggacgca ccctacctaa aggccgatgt
      181 tgttcggttt catcgggagc aatcactagc tgcggaagaa atcgatcaag agttgggaga
      241 cgcgagccgg ttcttcatgc tggagccaat gcgtggcgtt ggcgaaggtg aagagctccg
      301 cgctcgtgca atagcgcaaa ctttcgtgag ccatttcggt caggaggaaa tcgtcgcgct
      361 cgccccagag gatgcgggtg gggacgcgga cggttttgtc ctgaaatttg acgcgggtgc
      421 ggaagagggc ccggtaccag ttgatcacac cggttagggc accgggttgc gaccaagcgc
      481 tacgatattg ggcgagatct tcggcggaga acgtgcccgg tcggctggag cggagaagcg
      541 agcgggaacc gacacggaaa ttaaaggcgg agaaaagggc ttccggcagc caagggagtt
      601 gaaaaaagaa catgtaccaa ctgcgaagga attgacgcgg atgcgtgctt aggaatttgc
      661 gcatcacgga gggatgaggg acgttgagga caacgagctt ggcgatgcgc tgcggatgca
      721 atagcgcggt gctccaggca acggcggcgc cccaatcatg tccggcgaga aagatttttt
      781 cctggccgag ttggtcggca atagcgatga cgtcggagac aagttcagtt aaaacgtagg
      841 ccgccacgcc ggagggtttg ctgctgaggt tgtagccgca ctggtcggtg acgatgacgc
      901 gaaaacctgc agcggccaat ggctcgattt gtctacgcca gccataccag aattccggga
      961 agccatggag aaggaggacg acacggccgt ccggcggccc tgctgccacg gcgtgcagac
     1021 gcacagaagc gttttggaag aacagagttt ccactggcgt tgctctggcg cgccagtgta
     1081 gcagacgctc gcggatttac gacgcgtggg caaacggtct gcgcccaaga cgaaggaagc
     1141 cgcttgaatt ccctatttcg cctgcaagct ttcgatgtac acagcgagat ttcggatgcg
     1201 ctggtcgacc atcaccgggt tcgtaggatc gagtgactta aacagcggcc cccaaaccgg
     1261 catgtcggtg ctgccatggg atgccatagg cgaaccctgg cggagatcgt tcatgacgtg
     1321 gtccatgggg aacttaccgt tgtttttctt cgcgagttga gtgaggtcga cagggggagt
     1381 cttgagtgcg gatgcggccg ggccgtcgcc tttggcggag aggccatgac agggcgcgca
     1441 atagtcggcg aacatctggt ttccggaagt cgagttactt tgctggatgg gagtcttctt
     1501 gatcgttttt tcctggctcc atccctgact cgccaccaac aaagcggcgg caaaaaccgg
     1561 aaggaacaag aatccgatcc gagcgccgtg agggttaaac gaagaaagag ttttctgcac
     1621 gatgcacctc catgtgcaca gagagaatgc cgtggcagtc gcatacgttc ggtgatgggg
     1681 gtcacactgc gatgtgatgg aaatcacgca aggccaagca cctcccgcgc ccggaacgga
     1741 tgcttgagga gagtgtcttg tgcaacagag ggtgattcag cggcgctaga agcccggagc
     1801 gccggaagtg gcggggcgca tttcgggggc gttgacgccg aagcggagac gtgcgaagta
     1861 cgtttggccg agggacatgc agccggtgta caggtagccc cagacaaaga gcagcaggaa
     1921 cgggacggtg gcatagtttt cgttggaaat cgcgtaaacg atggtaagcg agaaatacaa
     1981 gccgagcagc acttccgcgt agggcatcca gccggctttg ttcctgtacg attttttcgc
     2041 gaaggtgccc tgcttccctt cgatattgaa ttttggtgtg cgggcaaact cacttttctt
     2101 gccgagcagt gcttcgatca ccgcttgcgc gttacgaacg gaaatcccta tgccagtggc
     2161 catgacaaag ggcaaataga gaaacgtgcg ccgccaggtc ttggggcgaa gctccctttg
     2221 cgccacgaga tagaagctgg agatggagca tgtggaagcg aggaacagcg gcaggtcgat
     2281 caccagcatc tggaaccagc cctgatagaa gcgcacgatc atggcgggca gcaacatggt
     2341 ggaaagcagg atcatcagcg ggtagctgat gttgccggta aggtggaaga aggcctcggc
     2401 tttgacgtgc cagggcgcat ccgacttcaa gactttggga aggatttttt ttgccgtctg
     2461 catgagacct ttggcccagc gcgcctgctg ggctttaaag ccgttcatgt ccacgggaag
     2521 ctcggaagcg cactcgattt gcgggaggta gagaaacttc cagcccttga gctgcgagcg
     2581 ataagaaagg tcggtgtctt ccgtgagggt gtcgtgctcc cagccgccgg catcgtcgat
     2641 ggcttggcgg cgccacacgc cggccgtgcc gttgaaattg aagaacgtgc cgcggcgcga
     2701 gcgcgcgccg tgctcgacca cgaagtggcc atcgagaagg atcgtttcca cgttggtcag
     2761 cagcgaatag ttgctgttga gatacgtcca gcgtgtttgc gccatgccaa ttttgccgcc
     2821 gccatcggga ttcatgaaat aaggaatcgt gcggcgaaga aaatcgggtt cgggaaggaa
     2881 gtcggcgtcg aaaatggcga cgaattcgcc cttggagatt ttcaggccgt tttccagggc
     2941 gccggctttg tagccttcgc ggttgctgcg gtgaatgtaa acgatgggga tgccctgcgc
     3001 gccgtgacgc tcgacgcaag cgcgagcgac ttcgcaggtt tcgtcggtcg agtcgtccag
     3061 gacctgcacg tcgagcagtt caggcgggta gtcgaagcgc gacacggctt cgacaagacg
     3121 ttcgatgacg tagcgctcgt tgaaaatggg aagttgaatg gtcacccgag gccagttggt
     3181 gaccggtggc ggagtcgcgg ggacattctt cgaatacgcg aaatagtcgt agaccagcca
     3241 atagcgatgg aggccgtaaa ccgcgaggac cagaaggacg atgaagtagg gaatcatcat
     3301 cgcgaggtcg aaggcattgg cgtggtacat gcctcggaac gtagggtccg taattttgtg
     3361 ccagtaatgg ctgatagggt tctgcgccgc gagagagacc gtaagtaaaa gtgtagtgtg
     3421 cacgtcgctg agctgatcca aagtccgcgc gtggcaactc gcgccgtcat cgggagacca
     3481 cgattataat gcggggttgc ttccagtcaa tgctcctgaa gcagccggag ctggtagcaa
     3541 accgttaagg gacgagcacg atttttccgg cggcgccggg ctcgaggatt tctttgtggg
     3601 cgcgcgcggc ttcggcgagg ggtaactcct tgccgaccac cgggcgcagg gtaccgtttt
     3661 cgaggccggc aatgaggccg gcgtggattt cggtttcttc cgccggcgta acgccccaaa
     3721 gggtgaaggc gcggatggag gcgcggcggc ccatgagttc gcgggcgttg atggtgactt
     3781 cgccgcggtt gccgatcacg atcacgcggc cgtggctggc cagaagtttt gtgtcgtggg
     3841 ccaggtttac gttggcgagc atttcgagaa tgatgtccac accgtggccg cccgtcgcct
     3901 tcaagatctc ttcctgatag cccgccttgc tgtgatcaaa aacctgatgc gcgccttccc
     3961 gctttgcgag gtcgaggcct ttttgcgttc caccagtacc aaagacagtg aggcccatgg
     4021 cgcgcgccat ttgcaccgcc gcgctgccga ctccgccgct cgcgccgtga acgagaaccg
     4081 tttccgccgc gcgggcttcc gccgaatggt gcagggcgtg gtacgcggtg ccatagggta
     4141 cccagacggc ggcaccttga ccgaaactaa ttttttccgg caaggaatgc acttgctctt
     4201 ccagcgcgag agcgtattcc gcgtaagcgc cagtgacggt cttcgccgtg tagacgcggt
     4261 cgcccggctt cactttttta acgccatcgc cggcggcttc gatgactccg gcggcgtctg
     4321 aacccggcgt gtagggaagc ggcggtttca cggggtaagt gcccgcgcgc atgtaggtgt
     4381 cgtagggatt cacgcccgcg gcatggatac ggacaagaac ttgtccggcg gctggttttg
     4441 gcgttgggac atcttcgagc ttgagaactt cgggaccgcc gaattcacga acgagaatgg
     4501 ctttcattgt ttttctccag cgaaaaaatt agcgaacgaa aaaaagtggc gtcaagccac
     4561 cacagtccag gaaggctaga aatggtcatc ggcgaccacc gtggccactt ctttgtcgat
     4621 gagtccggcg gactgcgccc aggcagtagt ctgccaattg tccttgcctt ttttcagagg
     4681 aatccacggc ggcagcgtct ggttcagata gttagccagc gcatagatgt aaggctcgta
     4741 cattttgcga aacccaatga gtttgcggtc ggcttcttct ccgtcatgga gtttcatgcc
     4801 gtgctgggcg aggatgtcgc ggatttggcg caagtcttgc gcggacagcc ggtcctgcgg
     4861 cagcggcttg ggcgcggtgc cgaagacttg cgagaggtca acgacggcgt gccgcgcaat
     4921 cgcaaaagta agttcggcct gtttttcaca aacgccctcg acaccaacga tgacgagcgc
     4981 gcacgtgtcg agaattgccg tgagcgaagc aatccaggat tggttgtcgt gctgcgagcg
     5041 aaaataggcg agcacgggat acgagagatg gctctccatc agctcggcgc accaaagctc
     5101 ccagtccttg aggagaacgc ggagggcgtc ctggccgtgg gcgtaactgt ggcggcgaag
     5161 cagttcgccg gcggtgggcg gcgtgccggc gcgggaatcg aggagggaga tattcacttc
     5221 gcgcttggag aacgacccgt agatgaaagg caagtagccg atgacggcgg caagaaaacc
     5281 gaaaccaaaa ccagcctcca aaacaaccat aacgcgggct aaaccggagc ggggaatgac
     5341 gtcgcccatg ccaagagtaa aaaaggtcgt tccgctgaga tagatatccg tcaggaaact
     5401 tggctggctg cccgtcataa ccaccgcgga cccagcgcca tattgcatga ggccgaagcc
     5461 aagcacgagg ccaacggccc atactcccac gagaacgagc agcgagagtg gcccatagaa
     5521 acccagcaag gcttctcggg tcttgggctt agggatgagg cagatcacaa atttccatac
     5581 gttccaggtg gagcggtaaa acaaacgagc gaggcggaac tttcgcgtaa cgcggcgcgg
     5641 aaggatgatg gcctcgaagg cgtcccagag cacgatcaga aagatcgcga cacccgcggc
     5701 gaacgctccc ggagctggaa tcatatgcgg cattgtacct gtttggccct ttcgatgaga
     5761 acgtccagcc aggacgattc gggagaattt ctcgcgagaa cgcctggatc ctacgattcc
     5821 agccgggcgg taacgctgat ttctgccttc aagagtttcg agacggggca gccggtttcc
     5881 gcggctttca cagccgcgtc gaatttggcc ttgtccgcgc cggggatttg cgcgacgaga
     5941 tccaggtggc ttttcgtgat ggcgaagcca tccgggagtt tttcgagcga aatcgttgcc
     6001 gtcgtttcga gcctgctcgc ggtcatgccc gcgttgccca actgggcgga aagcgccatg
     6061 gtaaaacagc cggcgtgcgc cgcggccagg agctcttccg gattggtgcc cacgccgttt
     6121 tcaaaccgcg tgctgaagga gtattgcgtc tgtttcagga cgccactatc cgttgaaatc
     6181 gagcccttcc catctttcaa accgccctgc caaaccgcgg atgctttgcg ctgcatggtt
     6241 tctccccttt tgactctttc actctttttg aatctagaga attcgattcc ggagcttctt
     6301 ggaagggtat attcagaaga tgaccttgtc gagaaagctt tgtgaacgtg ggtgaaggcc
     6361 gtcctgcagg aagtcgcaca ccgtccctgg ccgctgcccg cagcaccgtg ggtgatggcg
     6421 cagagctggc acaacctgct ctttgcgcac tggccggtcg acgccgcagg tttgcgcccg
     6481 cttgttcctc agcagttgca aatcgacttg tttgaagggc aggcttggct cgcggtagtt
     6541 ccgttttgca tgaccggtgt acggttgcgc gggacgcctg cggtgccagg gctctcggca
     6601 tttcccgaac tgaatgttcg tacctacgtg acgtgcggtg gaaagcccgg ctgtggtttt
     6661 tcagtctgga cgccggtaac gcgttggccg ttgccaccgc gcgggcgtgg tttcacttgc
     6721 cgtattttcg agcacgaatg agctgcgcag aacgggaagg ctggattcaa taccaaagcg
     6781 agcggacaca tcgcggaacg ccggccggat tgctggaggg ccggtatcgc cccgctggcg
     6841 aggctttttc tccgcaccac gagacgctgg agtatttctt gaccgagcgg tattgcctgt
     6901 atacgaccga tggacgcggc cagatcattc gcggcgagat tcatcatccg ccctggccgc
     6961 tgcagccggc cgaagcggaa tttgcgcgca actcgatgac ggagtcattg ggcattgccc
     7021 tgacttcgcg gcccctcctt cattttgcac gcaggcagga tgtcctagtg tggccgccgc
     7081 aaaggctttc tgccctggca gacgcctcct cgccgagctg ggtgacatga tcttaagact
     7141 ggaaggaatc cgctggattt tcagttttca ctgtttagtg ccagattcca cacaacggta
     7201 cttccgtccc ctttcgtggg cctgactccc tccgctactc tctaggaata tgctgtgacc
     7261 attgtttgtt cgccgctcgc ggaatttgac aagaatttcc ggaaaataga gaggatcgta
     7321 tgcgtacgag atcgtaccag ctccttacgg tttttctcgg atggatactt ttgcttccgg
     7381 ccaccactcg tggccaggaa aagacggtcc tggtcgagac cgggtccagc atgccggaac
     7441 ctctctataa gaactggatc gacgaattcc acaaacatca gccctccacg gacattcgtt
     7501 acatggccac gggaacggcg gagagcgcca gaaacattct tgcaggttcg ggtgatctcg
     7561 gcggcgggga tgcgcccatt ccggaggccc agttgcgcgc ggcaggccgg tcgattcttg
     7621 aattgcccgc cgtcttgatc gggattgtgg tcatctacga gctgcccgac gtaaagggcg
     7681 aactcaagct cacggggccg gtgctggcca gcatttttct cggaaaaatc aaatcgtgga
     7741 atgatccggc gatcgcgaag ctcaacccgg acatgaatct gccggggctt cccattcgag
     7801 ttctccaccg caacgagggt aagggctcca actacattct ttccgactac ctggccaaag
     7861 tgagtccgga attccttgcc gccgcggggc gcagcgaatc gccgaaatgg ccggtgggcc
     7921 agggcttcca gcgctcgcag gatctggtgg ccaagctgcg cggcacgcca ggcgcgatcg
     7981 gatacacgga actaaacctt gccgccggct ccgccgttcg catggctagc atcaagaatg
     8041 cggcgggtga gttcgtcaag ccctccgcaa actcgatcgc ggctgcggca accaccagca
     8101 gcagcaaaat gaacgatgat ttccgcgttt cactgacaaa tgcgcccggc aaagaaagct
     8161 acccgatcag cagtttcacg tggttttttg ttcctgccgt ggcccaggat cctggccggg
     8221 gcagcgcggt ggctgcctac ttgaagtggg tctacaccag cggacaaaaa atcgcccagg
     8281 aaaagggcta cgcgattttg ccccctgaag tcctgcaaaa agtcgtggcc aaggccgcaa
     8341 caattcactg aacgaagttg agtatttcgc ttggcgttgt ttttaaagca aagaaccgaa
     8401 ctcctcaggg gatattccgg cttggcgaag gacagagcgt agtgtgcctg tgtcgagttc
     8461 ggaatgatct ggcacgacca cttgtgcgaa tggcacattg cgtctaagaa taatgtggct
     8521 gccgtgctgc cgtttgattt gaaatcctag cttgagaagc gctgaaacgc agtcacgccc
     8581 cgagattcga ggcagcttgg tcataccgcg acaagaacgg catcaaaatt gtccggagga
     8641 acggcgagcc catcttcctg cagcgcgagg atataaccct ctatggcttc gcgaatattt
     8701 tgaatggcgt cttcttttgt ttttccttgg ctgacacacc cgggaagact cggacattct
     8761 gcgacccagt agccaatctc accgggatag aggattacct gtcgcatcaa aatacctctc
     8821 gagaaagtct accacgggcc tcacacgttg agcgtaaggg aaaccacgtc cgcccgcgtg
     8881 ggcggaggaa gcggcggcat ctttgagcgg tcgacaggat gatagagggt atcgcgctcg
     8941 acgggaacgc ggccggcggc gcggatcagc cgttcgagct cgttgcgcgg cgtgaactca
     9001 gacgttttcg cgccagcgtc gtgataaatt ttctcctcca caactgttcc gtcgaggtcg
     9061 ttcgccccga agcggagcgc gatctgcgcg atgctcggcg agagcatgat ccaatacgcc
     9121 ttgatgtgat cgaaattatc gagcagcagc cgcgacacgg cgatgttgcg caaatcggcg
     9181 taacccgtcg gcttcggaat gtgcgagagc gcggtgttgt ccggatgaaa tgccagcggg
     9241 ataaacgcca cgaagccatg cgtctcgtcc tgcagctcgc gcaacttgac gagatgatcg
     9301 acgcgctctt cttcggtttc cacgtgcccg tagagcatcg tcgcgttcga gcgcagcccg
     9361 atttcatgcg cctgccgcgc aatgttcagc cacatctgcc cgctgacctt gtgatcgcag
     9421 ataatccggc gcacgcgcgg atgaaaaatc tccgcgccgc cgccgggcag cgaatcgaca
     9481 cccgcttctt tcaatttgag cagcgtttct ttggccgaca gtttcgcgat gcgcgcgaag
     9541 tagcccacct ctaccatcgt gaacgccttc aagtgcacac ccgggcagcg ccgcttcagc
     9601 ccgcgaatca tctccaggaa atattcgaac ggcaaatccg ggtgcaggcc gccgacgatg
     9661 tggaattcca gcgcgccttc ccgggcgccc tgttcggcgc gggcgtaaat ctcttccaag
     9721 gagaagttgt acgcgcccgg cgcgctggga tcgcggccaa aggcacacag tttgcaatgc
     9781 gcgacacaaa tgttggtcgg attgatgtgc cggttgacgt tgaaatag
//
LOCUS       DASYVO010000483         6606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_251251, whole genome shotgun sequence.
ACCESSION   DASYVO010000483 DASYVO010000000
VERSION     DASYVO010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251251"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VGR03_16640"
     CDS             complement(<1..285)
                     /locus_tag="VGR03_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995962.1"
                     /translation="MLRNRFLILLGGCFLAASAVWADDVGFVDCSNHSEDTQVFGKPR
                     KTPEVVASIPCGERFTIIVYGFVFSRIQTGDGKIGYVYSNLIAVDRAATVA"
     gene            complement(651..1514)
                     /locus_tag="VGR03_16645"
     CDS             complement(651..1514)
                     /locus_tag="VGR03_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995963.1"
                     /translation="MKKSTKVISLTCLLLGISPGLFADRAHDLANQAGKQKPVTGCSP
                     ASLTTQTCHTKFPTGCTGKSFKYDAYLNFLKNQTPGASLASSAELGDADFKTLEGKVP
                     SGLTKGNHAKFAPALANLGEGNIFTVIAYLYFVEDTSKGANSGETTNCTLLLPDSFDY
                     HIGLGFDAALAKQILKSKPQPIFGKPVKMDKTSVVAEMTPHTRGPKWTFARVHSLQGQ
                     QVKVVGQLMMDNLHLNVKDDCSFPGAVASCWRSTVWEVHPVTQFYVCNLKAGCDKNSP
                     DTAWTSLDNVP"
     gene            complement(1759..2004)
                     /locus_tag="VGR03_16650"
     CDS             complement(1759..2004)
                     /locus_tag="VGR03_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995964.1"
                     /translation="MGIRRVRAFLAIIVVGVFMLITGTMAIYPLFGKTNVALNEYADF
                     FSKTASVYTGIIGVIVGYYFGRAEDPLPKDEHKPKEG"
     gene            complement(2016..2468)
                     /locus_tag="VGR03_16655"
     CDS             complement(2016..2468)
                     /locus_tag="VGR03_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995965.1"
                     /translation="MRVVFSVVVAAIAALCFPGLTPAQALFAPDPTPLERPAPLKTLK
                     SGDTAKICDAIAKALTDTGFKVTNQDCDVGDFEATKKTSTAGEFDKVLIWLERDFEKP
                     KDAVKLYFRYGRYETLAGHTEPVRIRTTAADEERNVGALKEALTSLKI"
     gene            2732..3292
                     /locus_tag="VGR03_16660"
     CDS             2732..3292
                     /locus_tag="VGR03_16660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1995966.1"
                     /translation="MPTEAGRGAEECFLVEAAQSDPAKFDALYEMHFERVYAFVAGRV
                     RDRATAEDVTSEVFHKALANLPSYEWRGVPFAAWLLRIAANAVIDHAKRAAREFPSPD
                     DPPEPAADPDLQASELRAIEHRAQLFRLVRQLPEVQKQVVYERFVEQRSIREIAQRLG
                     KTPGAIKQLQLRAVQNLRAQMEGGHG"
     gene            3285..4295
                     /locus_tag="VGR03_16665"
     CDS             3285..4295
                     /locus_tag="VGR03_16665"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1995967.1"
                     /translation="MAKRSLAMQLDEVVQAMLVLLRPRPEEAPDRDVAPLVRVAQILR
                     ELPREEFRATLKSDLQRRASMSEGTAASAAGTGEAQTRAVHYMRPGLTTITPYIIVRP
                     AAQFIEFLKMAFEGVERLRMPTPDGSIMHAEVEIGNGAIELSDGNEEYPTAPAAIHLY
                     VDDADATYERALRAGATSIAPVADQPWGDRWGAVKDQFDNHWYIAKPIGWTPGPEGLR
                     SVQPYLHLRDALKMIPFMEAVFGAESLGVAKSPEGTVLHGTMRIGNATLEISEAHGGI
                     QPMPCHLHVYVPDTDACYARALQAGASSIEVPQDKPYGDRAAGIKDAWGNSWFIATHL
                     GR"
     gene            complement(4377..4766)
                     /locus_tag="VGR03_16670"
     CDS             complement(4377..4766)
                     /locus_tag="VGR03_16670"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEV1995968.1"
                     /translation="MVPLSSTVSRQRSERRIAVRLPLKVRGRDARGFAFEEDTSSENL
                     CRNGAAFVTRFDVAIGSDLEIRIPFSHYASRRVDRFAGARNNDADFATQGRVVHVGDS
                     QPDGERMVGVQFTGPRFQRVFRSESAA"
     gene            complement(4720..5763)
                     /gene="dusB"
                     /locus_tag="VGR03_16675"
     CDS             complement(4720..5763)
                     /gene="dusB"
                     /locus_tag="VGR03_16675"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV1995969.1"
                     /translation="MIPASFQIRDLTIRPAAVLAPMAGVTDTLFRRVIRGLGGCGLLM
                     TEFTSSEGITRSAKKTLRYLYFQEDEHPITAQLFGANPAVMAEAAKMVEDLGYDAVDI
                     NLGCPAKKVVKCGGSGLLRDLPLLEEIFRSVRAAVKIPLTIKLRAGWDENSIVAVEVA
                     KMAESIGVEAVAVHPRTRTQGYAGAADWTIIAAVKQAVKIPVIGNGDINKPEDAARMV
                     TETGCDAVMIGRAAATNPWIFSQMQQHAQTGSYDTPDDAVRYRLLMDYYRQINAANLP
                     DGVGKMKQFACWFTHGVGNGSELRKIVHAARSPSEILESVECFFENRAAAGNEDPAHG
                     PAQLHRIEATLGT"
     gene            6140..>6606
                     /locus_tag="VGR03_16680"
     CDS             6140..>6606
                     /locus_tag="VGR03_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995970.1"
                     /translation="MKNKKPNAELVWKQLEDLLVPRLRLSVVDHTVYAHLLRHSRLEG
                     KLRLRFSIRWLSRNIRLSAASVRAGVRRLVAQGALRLVQRSKLGHVVEVRLPGEIRAV
                     RLNRIESRAADGAGAPTAVNLEEADFLQTKPLRKAIHARERGQCFYCLRRTTPT"
BASE COUNT         1324 a         1833 c         2093 g         1356 t
ORIGIN      
        1 cgcgactgtt gcagccctat cgaccgcgat caagttcgaa tagacgtaac ctatctttcc
       61 gtcaccagtc tgaatccggg agaagacaaa cccataaacg atgattgtga agcgctcccc
      121 gcaaggtata gatgcgacga cctcgggagt ctttctgggc ttcccgaaaa cttgggtgtc
      181 ttctgaatgg ttggagcaat ctacgaaccc cacgtcgtca gcccaaacgg cagaggccgc
      241 aagaaaacat ccccccagga gaatcaggaa tcggttgcgc aacatagccc cctcctcggc
      301 ccgagaagac cccgagccct aatgaagctt agcgggctgg agaggtgtgc gtcagatgga
      361 ccgagggcca ggatgttcca aaacggtact gtccaagatg ccgggggcac tgcctgtcgc
      421 atcctggtca ggaccctgtt ggaaaggatc agatttggag agttaaacta gcccaggcga
      481 accctgaacc atcaatcggt tgcaggcggg tgcgagcaga tataggggga gacgtgcggg
      541 tcaattttgg aactcgctta ccgcgaggcg gatttcaggg ctcgccgcga caaagcgtgc
      601 gatctaggtc gctcagtcag cgtgacccac gcgcgatcgc tcttttttct tcacggcaca
      661 ttgtcaagac tggtccaggc agtatctggt gagttcttgt cgcatcccgc cttcagattg
      721 cagacataaa actgcgtgac aggatggact tcccaaacgg ttgaacgcca gcaactcgcc
      781 accgcgcctg ggaagctaca gtcatccttc acgttcaaat gcaggttatc catcatcagt
      841 tgaccaacaa ctttgacctg ctgcccttgc agtgaatgta cccgggcaaa ggtccacttc
      901 ggcccccgcg tgtgcggcgt catctccgcc accacgctgg ttttgtccat tttcacgggt
      961 ttgccgaata tgggctgtgg cttggacttt agaatctgtt tcgcaagtgc cgcgtcaaac
     1021 cccaggccga tatggtagtc aaagctgtcc ggaagtagaa gtgtacaatt ggtggtttct
     1081 ccgctgtttg cacctttgct ggtgtcctcc acaaaataga gataagcaat caccgtgaag
     1141 atgtttccct cgccaagatt cgccagcgcc ggcgcaaact ttgcgtggtt ccctttggtc
     1201 aacccgctgg gaactttacc ttccagcgtc ttaaaatccg catcgccaag ctccgccgag
     1261 gaagcaagac ttgcaccggg cgtctgattc ttcaaaaagt tcaagtacgc gtcgtatttg
     1321 aaggatttgc cggtgcagcc ggttggaaat ttcgtgtgac acgtctgggt agtcagactc
     1381 gccggactac accctgttac cggcttctgc tttccagcct ggttggcgag gtcatgagca
     1441 cggtcagcaa acaggccggg ggaaatgccg agcaagagac aagtcaagct aatcacctta
     1501 gtggatttct tcatggttcc tccattggtt gcacgcgaag gccggtgggc ctcacaccag
     1561 gctcacctgc cacttctccc aaagggccgt ttgcaatcct atgtatcacc aagttaggta
     1621 taggtcaaaa taaattggcg gctggcgcag acgccgcgac gaggcaactt cacgacggcc
     1681 ggtatagaat cacgcggcta tggcgggagt ccggatcggg acgagcgcgc tcgcggctgg
     1741 aaggcggaga ggcgcgagtt agccttcttt cggtttgtgc tcgtccttgg ggagcggatc
     1801 ttccgcgcgg ccaaaataat agccgacgat cacgccgatg atgccggtgt agacgctggc
     1861 ggttttggag aagaagtcgg cgtattcatt gagcgcgacg ttggtcttgc cgaagagagg
     1921 ataaatggcc atcgtgccgg tgatcaacat aaagacaccc acgacgatta ttgccagaaa
     1981 cgcccggact cttctgattc ccatgatacc ctccgtcaaa tcttgagtga cgtcagagct
     2041 tccttgagcg cgcctacgtt tcgctcttcg tccgcggcgg tggtccggat gcggacgggt
     2101 tcggtgtggc cggcgagcgt ttcatagcga ccgtagcgaa agtagagctt gacggcgtcc
     2161 ttgggttttt cgaaatcgcg ctccaaccag atcaggacct tgtcaaattc cccggcggtg
     2221 ctggttttct ttgtggcttc gaagtcgccg acgtcgcagt cctgattcgt caccttgaaa
     2281 ccagtgtccg taagggcctt ggcgatcgca tcgcagattt ttgcggtgtc gccgcttttg
     2341 agcgtcttga gcggcgccgg cctttcgagc ggagtgggat cgggcgcgaa aagggcctgc
     2401 gcgggagtca ggccaggaaa acacaatgcg gcgatcgccg caacgactac gctgaagact
     2461 actcgcatgc cgtacctcct ccggaccacg cgattcctgc gagatattga gcctgctcgc
     2521 gttcgggagt caagtgccag attgcgagag ggcacgatcc gacaaggccg gagcgggtca
     2581 ccctgcggcg tgggtgggcg tgaccttttt cgggggcggc agttatattg ttagagccaa
     2641 gatggccacc cgacgaccgg cacggaaggg ttcaccgcca aagagcggcc cgcctcagaa
     2701 ggcgggccct acgaggctag gggatgaggg tgtgcccacg gaagcgggac ggggcgccga
     2761 ggagtgcttt ctcgtcgagg cggcgcagag cgatccggcg aaattcgatg cgctgtacga
     2821 gatgcatttc gagcgcgtgt atgcgtttgt tgcgggccgg gtgcgagacc gggccaccgc
     2881 ggaagatgtg acgtccgagg tgttccacaa agcactcgcc aacttgccca gctatgaatg
     2941 gcgcggggtg cctttcgcgg cgtggctgct gcggatcgcg gcgaatgccg tgatcgatca
     3001 cgccaaacgc gccgcgcgcg agttcccatc cccggacgac ccacccgagc ctgccgccga
     3061 tcccgacctt caagcctccg aattgagagc cattgagcat cgcgcgcaat tgttccggct
     3121 ggtgaggcag cttccggagg ttcagaagca ggtagtgtac gaacgatttg tggagcaacg
     3181 gagcattcga gaaatcgcgc agcggctcgg caagactccg ggtgccatca agcaactgca
     3241 attgcgcgcc gtgcagaatt tgcgggcgca gatggagggt ggccatggct aagcgctccc
     3301 tcgcaatgca gcttgatgag gtggtgcagg cgatgttggt gttgctgcgg ccacggccgg
     3361 aggaagcgcc agaccgtgac gtcgctcccc tggtgcgcgt ggcgcaaatt ttgcgcgagc
     3421 ttccacgcga agagtttcga gcaacgttaa aatccgattt gcaaaggaga gcatccatga
     3481 gcgaaggcac agcagcatca gcagcgggga cgggggaggc gcaaaccaga gccgtccatt
     3541 acatgcggcc gggattgacg accatcacgc cgtacatcat cgtgaggccc gcggctcagt
     3601 tcatcgaatt cctaaagatg gctttcgagg gcgtggagcg gctgaggatg ccgacgccgg
     3661 acggctcgat catgcacgcg gaagtggaga tcggcaacgg ggccatcgag ctgagcgatg
     3721 gaaacgagga gtatccgacc gcacccgcag cgatccacct ttacgtggat gacgctgacg
     3781 ccacgtacga acgcgcgctg cgggcgggag cgacctcgat cgctcctgtg gccgaccagc
     3841 cgtggggcga ccggtggggg gcggtgaaag accagttcga caatcattgg tacattgcga
     3901 agcccattgg gtggacgccg gggccagagg ggctgcgttc ggtgcagccg tatctacact
     3961 tgcgcgatgc tctcaagatg attccgttta tggaggcagt gttcggggca gagtctctgg
     4021 gcgtggccaa gtcgccggag ggcaccgtgt tgcacggcac gatgcggatc ggaaacgcga
     4081 cgctggaaat tagcgaggcg cacgggggga tccagccgat gccttgccac ttgcatgtgt
     4141 acgtgccgga cacggatgcc tgctatgcgc gggctttgca ggcgggggcg agttcgattg
     4201 aagtgccgca ggacaagccg tacggagacc gagctgcggg aatcaaagat gcgtggggga
     4261 atagttggtt tattgcgacg catttggggc gatagcgggg acacggaaac tattgggatg
     4321 gcagaagcga aaacagcccg cctcagaatg cgggccctac ggaagaaaaa gaaaaatcag
     4381 gcggcggatt cggaacgaaa aacgcgctgg aagcgggggc cggtgaactg gacgcccacc
     4441 atgcgctcgc cgtcgggttg agaatcgccg acgtggacga cgcggccctg ggtggcgaag
     4501 tccgcatcgt tgtttctggc gcccgcaaag cggtcgacac gtcgcgaagc gtagtgcgag
     4561 aaagggatac ggatttcgag gtcggagccg atggcgacgt cgaagcgcgt cacgaaggcg
     4621 gctccattgc ggcaaagatt ttcgctggag gtatcttctt cgaaggcgaa gccgcgggcg
     4681 tcgcgtcccc ggactttgag cggcaggcgg accgcgattc tacgttccga gcgttgcctc
     4741 gatacggtgg agctgagcgg gaccatgggc tgggtcctca tttccggcag cggcccggtt
     4801 ctcgaaaaag cattcgacac tttcgagaat ttcactgggt gaccgcgccg cgtgtacgat
     4861 tttgcgaagt tcgctgccgt tccccacgcc atgagtaaac cagcaagcga actgcttcat
     4921 tttgcctaca ccatcgggca ggttggcagc attaatttgc ctatagtagt ccatcagtaa
     4981 acggtagcgg acggcgtcat cgggggtgtc atagctgccc gtttgggcgt gctgctgcat
     5041 ctgcgagaag atccagggat tggtggcggc ggcgcggccg atcatcaccg catcgcatcc
     5101 ggtttcagtg accatgcgcg cggcgtcttc gggcttgttg atgtcaccgt tgccgatgac
     5161 gggaattttc acggcttgct tgacggcggc gatgattgtc cagtcagcgg cgccggcgta
     5221 gccttgcgtg cgggtgcgcg ggtggacggc gacggcttcc acgccgatgc tttcggccat
     5281 tttcgcgacc tcgacggcga cgatggagtt ttcgtcccag ccggcgcgca gcttgatggt
     5341 gagcggaatt ttgacggcgg cgcgcacgga gcgaaaaatt tcttccagca gcggcaagtc
     5401 gcggagcagg ccggagccgc cgcacttcac gactttcttg gcgggacagc cgaggttgat
     5461 atcaacggcg tcgtagccga ggtcttcaac cattttcgcg gcttcggcca tgacggcggg
     5521 attcgcgccg aagagctgcg cggtgatggg gtgctcgtct tcctggaaat acagatagcg
     5581 cagagttttt ttggcgctgc gcgtgatgcc ttcggagctg gtgaactccg tcatcagcag
     5641 gccgcagccg ccgaggccgc gaatgacacg gcggaaaagc gtgtccgtca ccccggccat
     5701 aggagcaagg accgcggcgg ggcggatggt caggtcgcga atttggaagc tcgccggaat
     5761 cataggttca gtttaggctg gtgcgcacag cgtagcaaga cgaggcatgg tccgtgttac
     5821 gcggcccagg ctacctagaa tttcctgaaa ttagggcttg caaagggttt tagttctggt
     5881 ataatgactc tcgggtgaat ctgcggctca accgcggttt cgcccttttt ctttttgcgc
     5941 cgcattctcc tccgggctcg cgctggtgtt aagtctttgt cccttctttt gcctgtgaac
     6001 tgttaaccgt cgactgtcaa attttttctc tgtgacctcc gtgctccggc ctctatgttc
     6061 tctgtgttga gtctatgtct cttctcttgc ctgtgaactg ttaactgacg atcgtcaaac
     6121 ttcgtttctg gatctaacca tgaaaaacaa aaaaccgaac gccgaacttg tctggaaaca
     6181 attggaagac ctcctcgtgc cgcggctgcg cctgtcagtc gtcgaccaca cggtctatgc
     6241 gcatctcctg cggcatagcc gcctggaagg aaaactccgc ctgcggttct cgattcggtg
     6301 gctgtcgcgc aatatccgac tatccgctgc atcggtgcgc gcaggagtgc gccgcctcgt
     6361 cgcgcaaggc gctttgcgtt tggtccaacg cagcaaattg gggcacgtgg tggaggtacg
     6421 gctacccggc gagattcgcg ccgtgaggct gaaccgcatc gaaagccggg ccgccgacgg
     6481 ggcgggcgcc cccacggcgg tgaacctcga ggaggcggac tttcttcaga ccaagccgct
     6541 gcgcaaggcc atccacgcgc gcgagcgcgg ccaatgcttt tactgtttgc gccgcaccac
     6601 gccgac
//
LOCUS       DASYVO010000484         8740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_181888, whole genome shotgun sequence.
ACCESSION   DASYVO010000484 DASYVO010000000
VERSION     DASYVO010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181888"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VGR03_16685"
     CDS             complement(<1..624)
                     /locus_tag="VGR03_16685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1995971.1"
                     /translation="MNKQTAVITGAGSATGIGFAIARRLYAAGVRVVITSTTDRIHAR
                     ARELDAGGEGVLSFIADLTVETDVQRFVDSVLSRAGRIDILVNNAGMAQTGRPADSKP
                     LIETSFAEWQNQISITLHTAFRMTRAVLPGMTQQKYGRIVNVTSVTGPLVSNSGSAAY
                     GAAKAAMDGMMRAVAIETASGGITINGVAPGWITTASSTESEKIAGLH"
     gene            826..1578
                     /locus_tag="VGR03_16690"
     CDS             826..1578
                     /locus_tag="VGR03_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995972.1"
                     /translation="MASSRILTGRGFLQEGITAQPAPPAGQNEFAHVGKSRTSPWKRI
                     FRIFRWTTYTFALVALVLLLHKAPPPLVETSPQAAARAEEKFEQVEKDLASGQPATLR
                     MDETELNSYLASHLALAGNGTPNPASGGSAQGAPNGATPTPQEVEQMRSNVRDVKVQL
                     IDDRVKAYVVFDVHGKDMTLQLEGRLGAQNGYLHFEPLSGQIGSLPIPQSTLASAVQR
                     LMDSPENREKLKLPSEISDLRIQNGEIVASYK"
     gene            1723..3630
                     /locus_tag="VGR03_16695"
     CDS             1723..3630
                     /locus_tag="VGR03_16695"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEV1995973.1"
                     /translation="MSEEKKEKDLGMGREITRRDFLNGVAIGVGGTLVGGGIGTETLL
                     AAAALDEFAPEKAPDYYPPARMGMRGNHDGTFTFAHRLRDGEGADSFGDAVNTGESYG
                     LVVVGAGISGLAAAYFFRKSAGRNTRILILDNHDDFGGHAKRNEFRAGGRMVLSYGGT
                     QSIESPGKYSDVAKALILDIGIKTEKFYKAYDQKLYSKMGTAAFFDKETFGEDRLVTG
                     MNATPWLEFLAKAPLSGAVRKDIARVYTEKKDYLAGMSVEEKSAQLTKISYAEYLTKH
                     CKLIPEALPFFQTFTHDLFCIGIESVPAMACYEAGDDYGSFTYAGFQGLGLPEGGKEE
                     PYIFHFPDGNASIARLLARSLIPEAIPGSTMEDVVTAKADYSKLDEAGGAVRIRLNST
                     VVHVQHTNTAKEVQISYVRGGKLQTVAAKNCVLACYNGMIPYLCPELPEKQKEALSYL
                     VKMPLVYSHVALRNWTSFAKLNVHHIVAPGGYHTYTALDFPVSLGQYRFPSNPEEPAV
                     LFMLRTPCKPGLPQRDQNRAGRAELLQTPFSKFERNIRDQLGRMLGGAGFDPAKDIEG
                     ITVNRWAHGYAFAPNPLFDPDWKEEEKPWVIGRKPFGKIAIANSDAGASAYTDVAIDQ
                     AWRAVGELLHG"
     gene            3716..5878
                     /locus_tag="VGR03_16700"
     CDS             3716..5878
                     /locus_tag="VGR03_16700"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV1995974.1"
                     /translation="MRVSKLCIVAAIFLGIVSAPILRAQTIHVDATPSHSTNTIKPTE
                     ALGAGIDRLPYGAADKLFVDDTIKQILSAGWQTVTYRQNTELHVEAWHWNPKGTWSDP
                     AGKGYFTGSATPGAEMVRHSYGYPLPHRGVTRDDGTDMEGYSRLDDGDENSYWKSNPY
                     LSKAYTGEDDSLHPQWVFFDLSSKQDITAIRIAWAEPFATKYAVQYWTGEDPIKKPAQ
                     GSWQAFPGGVVTNGKGGSATLQLATAPVDVQWVRIWMTASSNTCDSHGSSDKRNCLGY
                     AIREVYLGTTSKNGKFHDLLRHTPDQDQTATYCSSVDPWHEPGDLAERAGDQIGFDLF
                     YTSGVTRGLPAMIPIAMLYGTPEDAAAEIAYIEKRGYPISCVEMGEEPDGHYTLPEDY
                     AALYIQFATALHRVDPKLKLGGPIFTGENKDIEVWPDSDGKASFTGRFIDYLKSHGKL
                     GELAFFSFEHYPVDPGKVQWSSLYDEAYLVQHIMDVWREDGVPANVPFFITESNLSSA
                     ASEAYMDIWGGLWLADYIGAFLSGGGSAVYYFHYMPEPIDHGHNGSPGTFNFFSADKD
                     FKIRQPLSQYFVSQLLNLEWVQPGDGAHKLFPAASDIRDGAGHLLVTTYAVLRPDGQW
                     SLLIINKDQENAHTANISFDDAEKQTAATFGGPVSVTTFGKAQYAWHPNRNGGTADPD
                     GPALKSTVNAGPSTNFSLPAASVTVVRGVISAAKAGSK"
     gene            6086..6484
                     /locus_tag="VGR03_16705"
     CDS             6086..6484
                     /locus_tag="VGR03_16705"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV1995975.1"
                     /translation="MKSLFVGNMSFQTTESELRELFAPFGQVTRVHMAMDRETGRARG
                     FAFIEMPNDAEAGAAMTALDGKEVGGRNLKVNEARPKGEGGAPRGPRSFGGPGGGGAG
                     GGRGKGGGGRDRFSNEDYRESARQPREPRW"
     gene            6558..6740
                     /locus_tag="VGR03_16710"
     CDS             6558..6740
                     /locus_tag="VGR03_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995976.1"
                     /translation="MTTTFQKRQKEMKRIEKRKMKEEKRAQRKIDKPPASEVTYDPAS
                     DPTIDWNHSNEIGSPE"
     gene            6934..7311
                     /locus_tag="VGR03_16715"
     CDS             6934..7311
                     /locus_tag="VGR03_16715"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HEV1995977.1"
                     /translation="MQPGRRNLCALILVTVVAALLIVAPAGAHAVLLESNPSLKSAVL
                     GPDVPIKLRFNVRIDALRSRLTLIHPDGSAQSLQISKQTPADTLSTQATGLAAGAYRL
                     RWQVLASDGHITRGEIPFTVTPS"
     gene            7321..>8740
                     /locus_tag="VGR03_16720"
     CDS             7321..>8740
                     /locus_tag="VGR03_16720"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEV1995978.1"
                     /translation="MARLLQVFGFLSVLFRGATLTFQSLAVGGIVFLIFIVRRAKENS
                     AGLQPACLRWIRRSAIALAVMQISYVLANSLILRQSANMPLREVIGANFVLAGLLGIV
                     SAFTVIALTSARRSTGYTDLLLPAAAIITSSVMTSHSMARLDYRVPLVAFTALHQAAT
                     AAWLGGLPYLLIAIRRAPAPEFARQLTARFSQLALVSVAVLASAGLVLGFAYVGSFKA
                     VYGTSYGAMVATKALLFGLLLFLGALNFQLVRRGPASSILASLKRFGEAEIGIGITVI
                     LTAASLTSLPPAADLTHDRVTGPEIIARMSPRTPRFTSPSVHELPEDVYAAQKKAFEV
                     GSLSTESYVPGQTGTRPNTPAEKAWSEYNHHWAGIVVLAMGLLALVAQAGKVSWARNW
                     PLAFFGLSAFLFLRSDPETWPLGPIGFWATLADPEVLLHRFFAVLVIALAIFEWRVQT
                     GRVASGRARLVFPVLVAVSGALL"
BASE COUNT         1805 a         2625 c         2518 g         1792 t
ORIGIN      
        1 atgaagcccg gcgatttttt cggactccgt cgaggatgcc gttgtgatcc agccgggagc
       61 aacgccgttg atggtgatcc caccgctggc cgtttcgatc gcgacggcgc gcatcatgcc
      121 gtccatcgcc gcctttgccg cgccgtacgc cgcggagccg gaattgctga ccagcggccc
      181 cgtcaccgac gtgacgttta caatccggcc gtacttctgc tgcgtcattc cgggcaacac
      241 cgcgcgggtc atgcggaaag cggtgtgcag agtaatcgaa atttggtttt gccactccgc
      301 gaacgaggtc tcaatcagcg gcttgctgtc cgctggccgc ccggtttgcg ccatgcccgc
      361 attgttcacc agaatgtcga tccggcctgc gcgcgagagc acggaatcga caaatctctg
      421 cacgtcggtt tcgacggtga ggtcagcaat gaaggaaagc actccttcgc cgccggcatc
      481 gagttcgcgg gcgcgggcgt gaatgcgatc cgtggtggaa gtgatcacca cgcgcacgcc
      541 cgcggcatac aaccgccgcg cgatggcaaa accaatccct gtggcgcttc ccgcgccggt
      601 aatcaccgct gtctgcttgt tcaagggtag agtcattggg atgcccgctg aaggaatcat
      661 acctcgttcg cggaagattg tgattcgcgc gaatgagtga gattccctcc tggttgtgac
      721 gcgccgcggc acgcccgggc ggacagttgc cgaattcgcc tccaccgcac gtggttttct
      781 tgtcgccgac gcttccgagg tctacggttt ctttgtagcg tgagaatggc gtccagtcgt
      841 atcctcacgg gacgaggatt tttgcaggag ggaatcacgg cgcaaccggc tcccccagcg
      901 ggccagaatg aattcgccca tgtcggtaaa tcgcgcacca gcccgtggaa gcggatcttc
      961 agaatctttc gctggacgac ttacacgttt gccctcgtcg cgctcgttct gttgctccat
     1021 aaagcgccgc caccgctggt ggagaccagc ccgcaggccg ctgcgcgagc ggaagaaaaa
     1081 tttgaacagg tggaaaagga cctcgcgagc ggtcaacccg cgacactgcg catggacgaa
     1141 acggagttga attcctacct ggcatcgcac ttggcgctgg cgggcaatgg cacgccgaat
     1201 ccggcctccg gcggaagcgc gcaaggcgct cccaacggtg cgacgccaac gccgcaggaa
     1261 gtcgagcaga tgcgctcaaa cgtgcgggac gtcaaggtgc aactgatcga cgaccgcgta
     1321 aaggcctacg tcgtcttcga tgtgcatgga aaagacatga cgctgcaatt ggaaggccgg
     1381 ctcggcgcgc aaaacggata cctgcacttt gaacccctca gcgggcagat cggttcgctg
     1441 ccgattcctc agtccacgct cgcgtcggcg gtgcagcggc tcatggactc gcctgagaat
     1501 cgcgaaaagc tcaaactgcc ttcggagatt tcggatttgc ggattcaaaa cggcgaaatc
     1561 gtcgcgagtt ataaataatc aaaccccagg ctcgcagcat tcctgtaaat tagttcacca
     1621 ttgggatggc tccgcaaaga cgcgggaaag attgcttgaa atgtgtagag ttgtccggag
     1681 caggttataa tctgcggctg cggggtgcga taggggccag gtatgagcga agagaaaaaa
     1741 gaaaaagatc tggggatggg ccgcgaaatc acgcgccggg attttctgaa cggcgtggcc
     1801 atcggcgttg gaggaacgct cgtgggggga ggaattggaa cagagaccct gctcgccgcg
     1861 gccgctctcg atgaattcgc tccggaaaaa gcgccggatt attacccgcc cgcgcgaatg
     1921 ggcatgcgcg gcaatcacga cggaacattc actttcgccc atcgcttgcg ggacggcgag
     1981 ggcgcggatt ccttcggaga tgcggtaaat accggggaga gctacggcct cgtagtcgtt
     2041 ggcgcaggca tcagcggact ggcagccgcg tatttcttcc gcaagagcgc aggaagaaac
     2101 acgcgcattc tcattctcga caatcacgac gattttggcg gccacgccaa gcgcaacgaa
     2161 tttcgcgccg gcggccggat ggtgctgagt tacggcggta cgcagtccat cgaaagcccg
     2221 ggaaaatata gcgacgtcgc aaaagcgctg attctggata tagggattaa gacggaaaag
     2281 ttttacaagg cctacgatca gaaactctat tcaaagatgg ggacagcggc attcttcgat
     2341 aaggaaactt ttggcgaaga tcgtttggtg accggcatga atgccacacc gtggctggaa
     2401 ttccttgcca aagcgccgct ctccggtgcg gtgcgaaaag acatcgcccg agtctacacc
     2461 gagaagaaag attatctggc gggaatgtca gtggaagaga agtccgccca gctgactaag
     2521 atcagctatg ccgaatactt gacgaagcat tgcaagctga taccggaagc gctgccgttc
     2581 ttccagacgt ttacgcacga tctcttttgc attggcattg aatccgtacc ggccatggcc
     2641 tgctacgaag caggggatga ctacggctcg ttcacttacg ccggttttca aggcctagga
     2701 ttgccggaag gcggcaagga agagccctac atttttcatt tcccggacgg aaacgcctcg
     2761 attgccagac tgctggcgcg atccttgatc ccggaagcaa ttccaggatc gacgatggaa
     2821 gacgtcgtca ccgcaaaggc ggattacagc aagctggacg aagcaggcgg tgcggtgcgc
     2881 atccggttga acagcacagt cgtccacgtg cagcacacca acacagcaaa agaagtgcag
     2941 atttcctatg tgcgcggcgg aaagctgcag accgttgcgg cgaagaattg cgtgctggcc
     3001 tgttacaacg gaatgattcc ctacctttgt cccgaattgc cggagaagca aaaagaagcg
     3061 ctgtcgtatc tcgtaaaaat gccgcttgtt tattcccatg tagccctgcg gaactggact
     3121 tcgttcgcaa aactgaatgt gcaccacatt gttgcaccgg gtggatatca cacttacacg
     3181 gcgctggatt tccccgtgag cctcggtcaa taccgctttc cgagcaaccc ggaagagccg
     3241 gcggtcctct tcatgctgcg aaccccgtgc aagcccggac tgccgcagcg ggaccaaaat
     3301 cgcgccgggc gcgcggagct gctgcagacg cccttttcga aattcgaaag gaatatccgc
     3361 gaccaattgg gccgcatgct cggcggtgca ggattcgacc cggcgaagga catcgaaggc
     3421 attaccgtca atcgctgggc gcacggctac gccttcgcac cgaatccgct gttcgatccc
     3481 gactggaaag aagaggagaa accctgggtc attggccgga agccgttcgg caagatcgcc
     3541 atcgccaact ccgatgccgg cgccagcgcc tacacggatg tggccatcga ccaggcctgg
     3601 cgcgccgtcg gcgaactcct gcacggctag cagttcctcc agatgtatct tgacgcgggc
     3661 cggaaatccg ggttagatgc aaagcgcagc attaaaccgt tttaaagagt tcgacatgcg
     3721 cgtctccaaa ctctgcattg tcgctgcgat cttcctggga atcgtctctg cgcccatcct
     3781 gcgcgcacag actatccacg tggatgccac tcccagtcac tccacgaaca ccatcaagcc
     3841 gaccgaagcg ctcggcgcgg gcatcgaccg cctgccttac ggcgcagccg acaagctctt
     3901 tgtggacgac accattaaac agattctttc tgccggatgg caaaccgtga cctacaggca
     3961 aaataccgaa ttgcacgtgg aagcctggca ctggaatcct aagggaacat ggagcgaccc
     4021 ggcagggaag ggctatttca caggcagcgc cactccgggc gcggaaatgg tccgccactc
     4081 ttatggttat ccgttaccgc atcgcggcgt gacccgcgac gacggcacgg atatggaagg
     4141 ctattcgcgg ctcgacgatg gcgacgagaa ttcgtattgg aagagcaatc cgtacctttc
     4201 gaaagcctac acaggcgaag acgattccct gcacccgcag tgggtgtttt tcgatttatc
     4261 cagcaagcaa gacatcaccg ccattcgaat cgcctgggcc gaaccctttg ccaccaaata
     4321 tgccgtgcag tattggaccg gcgaggatcc catcaagaag cccgcgcagg gttcctggca
     4381 ggcctttccc ggcggcgtcg tgacgaacgg gaagggaggc tccgcgacgc tgcaattggc
     4441 aaccgcgccc gtagatgtcc agtgggtgcg catctggatg acggcatctt cgaatacctg
     4501 cgattcgcat ggcagctcgg acaagcgcaa ctgtctggga tacgccatcc gcgaggttta
     4561 tctggggact acatccaaga atggcaaatt tcacgatttg ctccggcaca cgccggacca
     4621 ggaccagacc gcgacctatt gttcctccgt cgatccgtgg catgaacctg gcgatctggc
     4681 tgaacgcgcc ggagaccaga tcggcttcga ccttttctac accagcggcg tgacgcgcgg
     4741 actacctgca atgattccca tcgctatgct gtatggcact cccgaagatg cagccgcgga
     4801 gattgcctac atcgaaaagc gcggttatcc gatttcgtgc gtggagatgg gcgaagagcc
     4861 ggatggccat tacacgcttc cggaagatta cgccgcgttg tacatccagt ttgctaccgc
     4921 gttgcaccgc gtcgatccaa agttgaaact gggcggacca attttcaccg gcgagaacaa
     4981 agacatcgaa gtgtggcccg acagtgatgg caaggcttcc ttcaccgggc ggttcatcga
     5041 ctatctaaag tcccacggca agctgggaga gttggcgttt ttctcgttcg aacactatcc
     5101 cgtcgatccg ggaaaagtgc agtggagcag tttgtacgac gaagcttacc tggtgcaaca
     5161 cattatggac gtgtggcgcg aggacggcgt gcccgcgaat gtgccgtttt tcatcaccga
     5221 gtccaatctt tcttccgcgg catcggaagc gtacatggat atctggggtg gactgtggct
     5281 ggccgattat atcggcgcgt ttctgagcgg cggcggcagc gcggtctact atttccatta
     5341 catgccggaa ccgatcgacc atggacacaa cggctctccc ggaacgttca actttttctc
     5401 tgcggacaag gatttcaaga ttcgccagcc cttgtcgcaa tacttcgtca gccagttgct
     5461 caacctggag tgggtccagc ccggcgacgg cgcgcacaag ctctttcctg ccgcaagcga
     5521 tatccgcgat ggagcgggac acctgcttgt gaccacctat gctgtgctgc ggcccgatgg
     5581 acagtggtcg ctgctgatca tcaataagga tcaggaaaac gcgcacacgg cgaacatttc
     5641 atttgatgac gcggagaaac agaccgccgc tactttcggc ggtcccgtaa gcgtaaccac
     5701 tttcggaaag gcgcagtatg cctggcatcc caatcgaaac ggtggcactg ccgaccctga
     5761 cgggccagca ctgaaatcca ctgtgaacgc cggtccgtcc acgaatttct cgctccccgc
     5821 cgcttcagtc acagtcgttc gcggcgtcat ctcggctgcc aaggccggtt cgaaatgaag
     5881 atgcggccat tcgcggcgaa gtgtgttact gtcgtgaagt agactcccga agtagaattg
     5941 cctgaattcc atgaagggtg tgttttgcgg tgctccctcc accgatttca gacaatttca
     6001 ggctaacgtc tcgtagtgtc tgggaagcgg cccttcacca agatcctgcg ctcgtggtgc
     6061 ttcgaagagt acagggagaa tcaagatgaa gagtttattt gtcggcaaca tgagcttcca
     6121 gaccacggag agcgaattgc gcgagctgtt tgccccgttc ggccaggtaa cgcgcgttca
     6181 catggccatg gaccgggaga ccgggcgcgc gcgcggtttt gcatttatcg aaatgcccaa
     6241 cgacgcggaa gcgggcgcag ccatgaccgc cctcgacgga aaagaagttg gcgggcgcaa
     6301 tttgaaagtg aatgaagcgc gcccgaaggg cgaaggcggc gcgccacgcg gtccgaggag
     6361 ttttggcggc ccgggcggtg gcggagcagg tggcggacgc ggcaagggcg gcggcggacg
     6421 cgaccgtttc tcgaacgaag attaccgtga gtcagcgcgc cagccgcgcg aaccgcgctg
     6481 gtagttttcc ttttccatgg atgggctgga cggcagggac cagggccctc gccgcactct
     6541 gctgtgagga gaggcttatg accacgacat tccagaagcg gcagaaagaa atgaagcgca
     6601 tcgagaagcg caagatgaag gaagaaaagc gcgcgcagcg aaaaatcgat aaaccccccg
     6661 cctccgaagt tacctacgat cccgcgtccg atccgactat cgattggaat cacagcaacg
     6721 agatcggttc gccggagtaa tttcgactcg cttcagcgca ggtttcaccc ggcggattct
     6781 ttatgctcat ccgcaggccg ccgagacagc cctgtctggg cagcggcgct cgaatgaatt
     6841 agcaatccac cccggcttct gtataatttt cttccagcag ccgaagccca aatttcacgg
     6901 agcttttcgc cattcttggc gaagaggaaa gtcatgcagc caggtcgtcg aaatctttgc
     6961 gcactcattc tggtgaccgt ggtcgcggct cttttgatcg ttgctcccgc cggtgcccat
     7021 gccgtactcc tggaatcaaa cccgtccctg aaaagcgcgg tattgggccc ggacgttccg
     7081 atcaaactcc gcttcaatgt gcgcatcgat gcgcttcgtt ctcgcctgac gctgatccat
     7141 ccggacggct ccgcgcaatc gctccaaatc agcaagcaga ctcccgcgga cacgctctcg
     7201 acccaggcca cgggtttggc cgccggagcg taccgcctcc gctggcaagt gctggcctct
     7261 gacggccaca tcacccgcgg cgaaatccca ttcactgtca cgccgtcctg atcgagattc
     7321 atggcccgct tacttcaagt ctttggcttc ctctccgtct tgttccgcgg cgcgacgctg
     7381 acgtttcagt ctctcgccgt tggcggaatt gtttttctga tcttcatcgt ccgccgcgcg
     7441 aaagaaaatt cggcggggct ccagccagcc tgtctgcgtt ggattcggcg ctcggccatc
     7501 gcgctggcgg tgatgcagat ttcctatgtc ctggcgaact cgttgatcct gcggcagtcc
     7561 gccaacatgc cgcttcgcga agtcatcggc gcaaattttg ttctcgccgg cctgttaggc
     7621 atcgtgtcgg ccttcaccgt aatcgctctt acttctgcaa ggcgctccac cggctacacc
     7681 gatctgctgc ttcccgcagc cgccatcatc acctcttcgg ttatgaccag ccactccatg
     7741 gctcgcctgg actatcgcgt gccactagtt gccttcaccg ccttgcatca agcggccact
     7801 gccgcctggc ttggcggcct gccatactta ctgatcgcca tccggcgagc gccggctccc
     7861 gagttcgcgc gccagctcac tgcccgattt tcgcaactcg cgctggtgag cgtggccgtg
     7921 cttgcttccg cgggtttggt gttgggtttt gcctacgttg gctcattcaa agcggtctac
     7981 ggtacttcct acggcgccat ggtcgccacc aaagcgcttc tctttggcct tcttttgttt
     8041 ctcggcgcgc tcaattttca actcgtccgc cgcggaccgg ccagctccat tcttgccagc
     8101 ctgaagcgct tcggcgaagc cgaaatcggc atcggcatca ccgtgatcct caccgccgct
     8161 tcgctcacgt cacttcctcc cgccgccgat ctcactcacg accgcgtcac tggtccagaa
     8221 atcatcgccc gcatgtctcc gcgaacccca agattcacca gtccctctgt tcacgagctt
     8281 cccgaagacg tttatgccgc tcagaaaaag gccttcgaag tcggctcgct atcgaccgaa
     8341 tcctacgtgc ccggccaaac cggaacacgc ccgaacactc ctgccgaaaa ggcttggtcc
     8401 gagtacaacc atcactgggc aggcatcgtc gttttggcga tgggactact ggccctcgtc
     8461 gcgcaagccg gaaaagtctc ctgggcgcgc aattggccgc tcgccttctt cggcctgtcg
     8521 gcatttctat ttctccgcag cgatccggag acctggccgc tcggccccat cggtttctgg
     8581 gcgactctgg ccgatcccga agtccttctg catcgttttt tcgcggtgct cgtgatagcg
     8641 ctagcgattt tcgaatggcg cgtccaaacg ggccgcgtcg cttccggccg tgcccggctg
     8701 gtttttcccg tccttgtcgc tgtttccggc gctctgcttc
//
LOCUS       DASYVO010000485        17990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_139440, whole genome shotgun sequence.
ACCESSION   DASYVO010000485 DASYVO010000000
VERSION     DASYVO010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 17990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17990
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139440"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2670)
                     /locus_tag="VGR03_16725"
     CDS             complement(<1..2670)
                     /locus_tag="VGR03_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995979.1"
                     /translation="MKSFRKSSRKPLAALGVVVLAVLTAGWAVTASGALPSWIRNIET
                     RTEIEAAFFRLMSLPGGDVLFRRPPRETRAALGDLLKKQPKDAELFSLRALEDEQQLD
                     FAAAESDWKSYAGNAADKGAAHIALADFYHRRLRPLDEIKALSVVAALPSDASEKLIP
                     SAEQRSWRAFERIFEVVRAQGLSKDVSVAQYRAWLVRYPRQPSLYARFLEYLIAQKDY
                     AAATQLVADYRREFPGDEIFPIKAEALVEYRQGSLQQGLAVYEKSFQPLWAPELIKSY
                     FDLLTETQSLRKFLDNARAAQSANPQDLNAMARIFYYYQQQGKLDAAQETIAKFRLRK
                     DAAKSAWTAQELYVCARLLGDVHAYPEAARYYFALYNAKGMPDAQERALAGLASLLLT
                     APETPIRLGAGELSMYRDIATMDQGPGFLNGILSLLLNSTEPGTEFSQEEQRAVPYFH
                     RARAAQLLNLLDTRYPNSPRRAELHATLLDFYAASGESEAVIKGGSDFLTAFPNAPQR
                     PAVALLMADAYSRTGKVPQEFAIYDSLLQELAAKAEGVPLGSGAAVSGENLPPEAVTP
                     PPEMSEDSEQPPEGDDAEAASPSRAPQRRTNSAFQVAAAARHTPVRARSPEYARVLER
                     YLARLAELKQVPQALTVLRRELDHNPGDPGLYERLAVFLDQNRLGEEQAEIYRRAIAR
                     FPDRSWYHKLARFYLRHQQNAQFEKLTQEAVRIFDGTDLERYFQSLVGGSPMMYLRLN
                     LYAHDRFPHNPVFVRNLLSAYTRAETWDPAAWEALLRQHWFEETELRDEFFEYLTRKG
                     KLESELKALQQAEPASASGKWNEFVQQNPAAGNYVAQAHLWRSHFEESAPVLEALAQE
                     YPADPEIGRTASSVFRSLAYFDSADTGIAVKIEG"
     gene            complement(2654..5059)
                     /locus_tag="VGR03_16730"
     CDS             complement(2654..5059)
                     /locus_tag="VGR03_16730"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEV1995980.1"
                     /translation="MKRTHIAVIVLLLFLVPALLLADGGVLIPRDKAQPDPAILSLEE
                     MEITIRIDNGDARVFVRQVFANHTNHIEEGNYVFALPSRATVSDFAVWDGPTRIPAVI
                     LERKRAEEIYNQLKQQAIDPGLLQMGERGEEEAKRSSIFSARITPIPPYGTKRLEFEY
                     HESIPAENLKSYFAIPLRPDAYQAQVARHLWINFELRSSHAIANFQVAAKTFPLQFDQ
                     NTPHLVKGRFEGQNVNLTEDFVATFDLDRAGSDTLQVLTYRNPVTGQPSPTDTAPVRS
                     TNEPGFFAVEALLGLAQQHQPAPVPGGNTAPSSTPAKTLIFLFDTSLSMQWEKLERSY
                     QALETLLRRLQPSDSFNLLLFNSQTQAFQPAPVAADIAGIQRALDFVRASRLRGGTDL
                     QRALEDGLKQCAVSGGGSRYLVLLSDGGATRGPIQNGKIAAWYEASWKKLPVAQRPRT
                     YLFGVGDDANLPLLRMLARQDGLSEHVLSTEPIDFKLNSFVSKIGRSPVGGLRLETVQ
                     DAGIDAVYPLQDSAYSGSLAAWVGRYQKPRQDAVFTVRGVRDGNPLEISGKVNLPRES
                     LDHPQLPRLWARARVDALLEKIQREGEDQATIDEIIRLARQYKFVTPYTSFLAVPRAL
                     LRPRVIRPGDPVLRVKTDKSIVSVVALFPFGLVQQLRHLSGEDIWQTRFFAPTDMQDG
                     TYSVRLILRDRSGHTYREAKTFVIASKPPVIQIKLDGRRFRRGQVLDLKVSASQSTRT
                     LIARLEGAVPVGLRWDARAGASTGQLVIPEQAIPGIYRLTVTGEDIAHNIGSQGVDIE
                     IVP"
     gene            complement(5056..6966)
                     /locus_tag="VGR03_16735"
     CDS             complement(5056..6966)
                     /locus_tag="VGR03_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995981.1"
                     /translation="MSWRTVAAGGLALIVLAAGFVGWRVSRALRLSTQEVRSEQEIHF
                     IVRPLAPPADLGFEAVSAPAVFLQVARFQDQLYIAGPAGLLEYDLGGTPLRQFSVGRD
                     LPPSLVVALAPAVLADSREPELVVATSQDGVLAFNGRSFRQIYPQDAAARAITSILPV
                     AAGRLLIGTKKRGVLLYDGKRIAALHPTLSNLYITALAGTESDLWIGTLNQGVLHWHA
                     GATESFNEQQGLPDRQVQCVATSGDKTYVGTVLGVAVFDRGRFSHVLAPGVLVTSLFS
                     TPTNLIVGSENQGVITVPLEKRRSGPGANASSGLSDVRQFFESGEVVYVLTRNALYRM
                     TARGLGWQQVLKPGPAILSDRNISALAADASGRLWIGYFDRGLDLLQAGAPRATHVEN
                     DHVFCVNRILPDPRTGTVAVATANGLVRFGVSGRQEQVLTRADGLIADHVTDVALYRD
                     GLAVATPAGLTFLDASGARSIYAFQGLVNNHVYALGVSGDELMIGTLGGLSVFGKDAV
                     RVNYTAGATSGLKHNWITAVAPVGDEWMVGTYGAGVLGLDRSGHFHPFDKATAPFEVN
                     PNAMLVTPRHVFAGSLGKGLYVYDRESARWTTITGGLPSPNVTALAESSGYIYVGTDN
                     GLVRIQEQRLQP"
     gene            7511..8122
                     /locus_tag="VGR03_16740"
     CDS             7511..8122
                     /locus_tag="VGR03_16740"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEV1995982.1"
                     /translation="MRKVKTEMPAVLTIGHSNRTWKDFLKLLRAHRVERIIDVRSIPR
                     SRHNPQFNRETLSTKLRAARIGYVYLRKLGGLRHARRDSPNMGWHNTSFRGFADYMQT
                     SEFEAGLHRLMKLSGQKRSAVMCAEAVPWRCHRSLIADALTVRGIQVDDIMSMKRSQV
                     HSLIPFARVQGHRITYPAGNRGGGRVSPIRFRESSRKNRKTKV"
     gene            8134..9621
                     /locus_tag="VGR03_16745"
     CDS             8134..9621
                     /locus_tag="VGR03_16745"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV1995983.1"
                     /translation="MMPLDYPLWLRAAHFFNFLLLSLLVRSGLEILSAHPRLYWNDHC
                     TPGSEWLKFTKKQRPEDRLWTASDEETSFPSAIALPGHENLGLGRHWHFLADFAWLLT
                     GLTYIVMLFVTPEWRRLIPTSWQIVPDAWHAMLGYLSLHIVETPGGYNALQQLSYAAV
                     IFLLAPFSIATGVAMSPAVAARFPWYIKIFHGRQGARSLHFLALCTFIVFFIGHVTIV
                     ALHGFRRELALIVLGQTHDPQLTLALVVWLSGLTGIVIIHTVTTVCSRRRPRFVQKAT
                     QSITDPVRTLLFGHEISAQHYSHADISPYFWVNGRPPIEETFLAMARENFANYSLEIG
                     GLVERQLHLTLADLRAMPKQTQITKHCCIQGWSGVAEWGGVSLHHIVALCRPLPAARY
                     IVFYAFDNKSTSEPHAQGPGYFYGTIRLELAEDPQTILAYEMNGQPLPIVHGAPLRLR
                     VETQLGFTMVKYIRAIEFIEDYKTIGQGQGGWREDYQYFSQEAGI"
     gene            complement(9669..11054)
                     /locus_tag="VGR03_16750"
     CDS             complement(9669..11054)
                     /locus_tag="VGR03_16750"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEV1995984.1"
                     /translation="MAIPLEAPVAAAKNERRASLSPAEVMDLARAREASLSRLLMAYI
                     SSGLVFMLLPGTFLGVWNLLQISGRESVASVSPAWLQAHGHAQVFGWIGSFIFGIGFY
                     SIPKLRGAAKPACGAAWACWAIWTIGVAARWAANVYGWQWRLLLPLSGVLELAAFVIF
                     FGAVSQHRPEDPGKNRRDAWIWVVVSASTGLMLVLIANLAGNIYLALQGASPAWPHGL
                     DQRYVVLVAWGFLVPFVWGFSTKWMPVFLGLKPIRPRLLWSALVVNFAGVVLTLVGRG
                     AEATVLFVVGAALVIAALRMFEAAQQEPKTRGVHWSFPFFVRMAYGWLLGAALLGVGA
                     ALWDSSGGIWGASRHALTVGFIAVMVMCVGQRILPAFAGMRLLWSTRMMFAGLALLSI
                     GCALRVSSEVLAYQGYANWAWSVLPVSAVLELAGITAFAMNILGTFLLEPSHAQKQPL
                     VVGINQIISEG"
     gene            complement(11064..11786)
                     /gene="ric"
                     /locus_tag="VGR03_16755"
     CDS             complement(11064..11786)
                     /gene="ric"
                     /locus_tag="VGR03_16755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HEV1995985.1"
                     /translation="MNFNSETKVKEIALANPGATRIFEEAGVDYCCGGDKSLHDACAH
                     ADVSSEEILNRLRESSEQVGPADAQWISAPLGELTQHIREKHHRYVREGIVRVRALAA
                     KVKAKHGENHPEIADIEKHFLVLGQEMIMHMQKEEQILFPYIEALERSVKGNGTLEPP
                     FFQTVRNPIHAMMKEHDSAGDLLKQIRKASSEYTAPADACPSYRALYQDLREFEADLH
                     QHVHLENNILFPRAVEMEAAAA"
     gene            complement(11883..12578)
                     /locus_tag="VGR03_16760"
     CDS             complement(11883..12578)
                     /locus_tag="VGR03_16760"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV1995986.1"
                     /translation="MNENIEMVLRKTPLFASLTEQEMHALATRVSKKHFQRGELLFCE
                     GDSCTGLFLVATGKIRIFKLSPAGREQVLAVEEPGSSFAELPVFDGGNYPASASALED
                     AEVLFISRKDFQNFCREHPEVALKVIAVVGSRLRRLVGIIEDLSFTTVRYRLIALILR
                     LTQASGTTSKEGVHVELTKTHQDLAAELGTVRELVSRNLSRLHAEGFLEVDGRRIIVK
                     DLAGLKREQSASE"
     gene            12776..12852
                     /locus_tag="VGR03_16765"
     tRNA            12776..12852
                     /locus_tag="VGR03_16765"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12810..12812,aa:Arg,seq:tct)
     gene            13638..14552
                     /locus_tag="VGR03_16770"
     CDS             13638..14552
                     /locus_tag="VGR03_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1995987.1"
                     /translation="MARWHKNFFLILLFFLGGGAGLEAQMDPYFTAINHPVPQDTMMV
                     MLLSDFQSARSTNDFFTGMSMVQYGLTPRWTVGFMAEGQKIFGLPTTYGGLRINSYFR
                     VFPKDHLLNFTLYGEYEGLNGAALYKMEVAGFGGEDLNEPLATARRTHVRTFEQRAIM
                     YHDWGRVNLTFNFINETDLENGENDFGYAWGVFRQAAYTTMETDKGMAGMADKSKMKV
                     PPVFSFQRLGYGFEMIGALGNTHQFGFDWPRQQQYMGPVFSYSLSKRWTVQVEPAFGL
                     SHVSDPFVLRMGVGYSIDHLLHRRSPTP"
     gene            14874..15959
                     /locus_tag="VGR03_16775"
     CDS             14874..15959
                     /locus_tag="VGR03_16775"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1995988.1"
                     /translation="MRKTFIYAAVAALLLAAGTVPAWAQASTLIVKGKVTLNGKPFAN
                     AQVVLTKPETGKTLKTKTDKNGEFILAGVELATYEVTVLGKDGEQLSKNETTFEELHH
                     KETILTIEINDTGGGSGEKGLTGPPGPQPKLSKEQREEMKARRAKIEPLVAKVQEAYG
                     QKNWLAAETALRELIAADPEHWGPYQALGNTQFNLGKYQEAVQTYEIGVQKAQKTSPD
                     PQDPTSDPSKIKAGIGQMLTQQGNAYLKLGKTNEAVAAFTKAAPMSANPAIAYYNLCV
                     VLYNSGQLQDVGPACDKAIAADPKRPDAYFIKGRALYENSETDASGKHLAPAGTIEAL
                     KKYLELLPDGPHATEAKLMLEGIAAPN"
     gene            15956..16261
                     /locus_tag="VGR03_16780"
     CDS             15956..16261
                     /locus_tag="VGR03_16780"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV1995989.1"
                     /translation="MSFRRAAAEFLLAGAIGNLFAVAERYPALRAVESVLLLQEQLTS
                     PENRIAYARQFYDDEVMKYNTWLSTFPHNLIARGLGFAPAVMFTAEEADLVSPQVKL"
     gene            complement(16335..>17990)
                     /locus_tag="VGR03_16785"
     CDS             complement(16335..>17990)
                     /locus_tag="VGR03_16785"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV1995990.1"
                     /translation="ATPMMCARFLRHDTGKHGATYRVAERSFDGLLHTYDIGLRWVLR
                     HQLLVLFITFATIGVNIYLFWIVPKGFFPQQDTGRISGFVQADQDISFSAVSEKMRAY
                     VKIIMEDPAVDTVTAFTGGSGGTNSARMFMQLKPIQERKISADQVIARLRGKLARIPG
                     ATLFLQAVQDLSVGGRFGGAQYQYTIQADQIADLNQWAPVMMKRMSRIPALRDINSDQ
                     QIHGLQAQLVVDRTTASRLGVSFQAVDNALNDAFGQRQVSNIYMGLNQYHVVLEVLPE
                     YQQNPEGLNVIYVASTTGKLIPLSAFAHFEHSSSPLTVNHQGQIPSITLSFNLAPGAS
                     LGPVVTQIQAAQREIGMPANVHGSFQGTAQAFQDSLSSQPVLILTALAAVYIVLGVLY
                     ESYIHPITILSTLPSAGVGALLALFLFKTDLNVIALIGIILLIGLVKKNAILMIDFAL
                     QAERVDGKSPVDAVYQACLLRFRPIMMTTMAALLGALPLALGRGTGSELRQPLGITIV
                     GGLIFSQMLTLFTTPVVYLYLDRLRTSLRRSGVGQPVQAQPAD"
BASE COUNT         3811 a         5223 c         5451 g         3505 t
ORIGIN      
        1 gccctcgatt ttcaccgcga tgccggtatc cgcggaatcg aaataggcca gcgagcggaa
       61 aaccgaggat gccgtccggc cgatctccgg atccgcggga tactcctgcg caagggcctc
      121 gagcacggga gcgctctcct caaaatgaga gcgccagaga tgcgcctggg cgacgtagtt
      181 cccggcggcg ggattctgtt gcacgaactc attccacttt ccactagcgg aagcgggctc
      241 ggcctgctgc aacgctttca actcggattc gagcttgcct ttgcgcgtca gatattcaaa
      301 aaactcatcg cgaagttcag tctcctcgaa ccagtgctgc cgcaagagcg cctcccacgc
      361 ggccgggtcc caggtttccg ccctcgtata agcggacagg agattgcgga caaaaacagg
      421 gttgtgggga aaccggtcgt gggcataaag attcaagcgg agatacatca tcgggctgcc
      481 gccgaccaat gactggaaat agcgctccag gtcagtgccg tcgaaaatcc tgacggcctc
      541 ctgtgtcagc ttctcgaatt gcgcattttg ttgatgcctc agatagaaac gggcgagctt
      601 gtgataccag gagcggtccg gaaatcgcgc aatcgcgcgc cggtagatct ccgcctgttc
      661 ctcgcccagg cgattctgat ccagaaagac cgccaggcgt tcatacaggc cgggatcgcc
      721 ggggttatgg tccaattcgc ggcggagcac agtgagggct tgcgggactt gcttgagctc
      781 ggccagccgc gccagataac gctcgaggac gcgcgcgtac tccggagagc gagcgcgaac
      841 cggcgtgtgc cgtgcagccg cggcgacctg aaacgcagag tttgtgcgtc gttgcggagc
      901 gcgtgatgga ctggctgctt ctgcatcatc tccttccggt ggctgttcgg aatcctcaga
      961 catctccggc ggcggggtga cggcctcagg gggaagattt tccccgctga ccgctgcccc
     1021 ggacccgagc ggcacacctt ctgcctttgc cgccagctcc tgcaggagag aatcgtagat
     1081 ggcgaactcc tgcggcacct ttccggtgcg ggaataggca tccgccatga gaagcgcaac
     1141 ggcagggcgc tgcggggcat tcgggaaagc cgtgagaaag tcgctcccgc ccttgattac
     1201 cgcttcgctc tcgccggatg cggcgtagaa atcgagcagg gttgcgtgaa gctcggcacg
     1261 gcggggagag ttcgggtatc tcgtgtcgag gaggtttagg agctgcgcgg cgcgcgcccg
     1321 atggaaataa ggcacggcgc gctgctcttc ctgcgagaat tccgtgccgg gttctgtcga
     1381 attcagaagg agtgaaagga tgccgttcag aaacccgggc ccctggtcca tcgtggcgat
     1441 gtcccggtac atggagagct cgccggcgcc gaggcggatg ggagtctccg gggcggtgag
     1501 caagagactc gcgagcccgg cgagagccct ctcctgagca tccggcatgc ctttggcgtt
     1561 gtagagggcg aagtagtagc gcgccgcttc gggataggcg tggacgtctc cgagcaaccg
     1621 cgcacagacg tagagctcct gcgcggtcca ggcggacttg gccgcatcct tgcggagccg
     1681 aaacttggcg atggtctcct gcgcggcgtc gagcttcccc tgctgctggt agtagtaaaa
     1741 gatgcgcgcc attgcgttca ggtcctgggg gttggcgctc tgcgccgcgc gcgcgttgtc
     1801 gaggaacttg cgcaagctct gcgtctcggt gaggaggtcg aaatagctct tgatcagctc
     1861 cggggcccag agcggttgga agctcttttc atagaccgcc aggccttgct ggagcgagcc
     1921 ctggcgatac tcgacaagcg cttcggcctt tatgggaaag atctcgtcgc cgggaaactc
     1981 cctgcggtag tccgcaacga gttgggttgc agcggcataa tctttttgcg cgatcaagta
     2041 ctcgagaaat cgtgcataga gcgatggctg gcgcggatag cggacaagcc aggcgcggta
     2101 ctgcgcgacc gaaacgtctt tggacaagcc ttgcgcgcgg acgacttcga agatgcgctc
     2161 aaaagcccgc caggagcgct gctccgcgga aggtatcagc ttttccgaag catcggatgg
     2221 cagagcggcg acgacggaga gcgccttgat ctcgtccaag gggcgcagcc ggcggtgata
     2281 gaagtcggcg agggcgatgt gcgcggcgcc cttgtccgcg gcgttcccgg cgtaactctt
     2341 ccagtcggat tcggcagcgg caaaatcgag ctgctgctcg tcttcgagcg cgcgcaagga
     2401 aaaaagctcc gcgtctttcg gctgtttttt gagcaggtca cccagagcgg cgcgcgtttc
     2461 gcgcggcggc cggcggaaaa ggacgtcacc cccgggcagg gacatcaggc ggaagaacgc
     2521 cgcttcgatt tccgtgcgcg tctcgatgtt gcgtatccag gatggcaagg ccccgctggc
     2581 tgtgaccgcc caacctgccg tcaaaacggc aagcacgact acgccaaggg ccgccagggg
     2641 ctttcgcgat gacttacgga acgatttcaa tgtccacccc ctgcgagccg atgttgtggg
     2701 caatgtcctc acccgtcacc gtgagccggt aaattccagg gatcgcctgc tcgggaatga
     2761 cgagttgccc cgtgctggcg ccggctcgcg catcccatct gagcccgacg ggcaccgcgc
     2821 cttcgaggcg tgcgatgagg gtacgcgtgc tctgcgaggc ggatactttc aggtccagga
     2881 cctgcccgcg acggaaacgc ctgccatcga gcttgatctg aatcaccggc ggcttgctgg
     2941 caatgacgaa ggtctttgct tcgcgataag tgtggccgga gcggtcccgc agaatgagcc
     3001 gcacgctata ggttccatcc tgcatgtccg tgggagcaaa gaaacgtgtt tgccagatgt
     3061 cttcgccgga aagatggcgg agttgctgga cgagcccgaa gggaaacagc gcgacgacgg
     3121 aaacgatgga tttatccgtc ttgacccgca gcacgggatc tcccggacgg atgacgcgcg
     3181 gccggaggag cgcccgaggc acggcgagga acgaggtgta gggcgtgacg aacttgtact
     3241 gccgggccag ccgaatgatc tcgtcaatgg tggcctggtc ctccccttcg cgctggattt
     3301 tttcgagtag cgcgtccacg cgagcgcgcg cccagagcct cggaagttgc gggtgatcga
     3361 gcgactcgcg cggcagattg actttgccgg agatctcgag ggggtttccg tcgcgaaccc
     3421 cgcgaaccgt gaagacggca tcctgccgcg gcttctggta acggccgacc cacgcggcga
     3481 gcgagcccga ataagcggag tcctgcaacg gatagacggc gtcaatgccg gcgtcctgga
     3541 cggtctccag gcggagcccg ccgacgggac ttctgccgat tttcgagacg aaggaattca
     3601 gcttgaagtc aatgggctcg gtggaaagaa catgttcgga caggccatcc tgccgcgcga
     3661 gcatgcggag caaaggcaag ttggcgtcgt cccctacccc gaaaagataa gtcctcgggc
     3721 gctgggctac ggggagtttt ttccaactgg cttcgtacca cgcggcaatt ttgccgttct
     3781 ggatcggccc gcgcgtcgcg ccgccgtcgc tcagcagaac gagataacgg ctgccgcctc
     3841 cgctcaccgc gcactgcttc aagccgtcct cgagggcgcg ctgcaggtcc gttccgccgc
     3901 gcaaccggct agcacggacg aagtccagcg cgcgctgaat tccggcgatg tccgcggcca
     3961 ccggcgcggg ctgaaatgcc tgcgtctggc tgttgaagag caggagattg aagctgtccg
     4021 aaggctgtaa cctgcgcaac agagtttcca gggcctggta actgcgctcg agcttctccc
     4081 actgcataga gagagaggta tcgaagagaa agatcagcgt cttcgcggga gtggagctag
     4141 gcgccgtgtt tccgccgggg acgggagccg gttgatgctg ctgcgcgaga ccgaggagcg
     4201 cttccaccgc gaagaagccg ggttcgttgg tgctgcgcac gggagcggta tccgtagggg
     4261 atggctgccc ggtcacggga ttgcgatagg tgaggacttg cagggtatcg cttccggcgc
     4321 ggtccagatc gaaggtggcc acgaagtctt cggtcaagtt gacgttttgt ccttcgaagc
     4381 ggcctttcac caggtgcggc gtgttttgat cgaactggag cgggaaagtt ttcgccgcga
     4441 cctgaaagtt cgcgatggcg tgcgaggaac gcaattcgaa attgatccag agatgccgag
     4501 ccacctgggc ctggtaggca tccggccgga gcggaatggc gaagtaggac ttgagattct
     4561 cggccgggat ggactcatgg tattcgaatt cgaggcgctt cgtcccatag ggcggtatgg
     4621 gagtgatgcg ggcgctgaaa atggagctgc gcttggcctc ttcctcgccg cgctcaccca
     4681 tctgcagcaa tcccgggtcg atcgcctgct gcttgagctg gttgtaaatc tcctcggcgc
     4741 gcttgcgctc gaggatgacg gcgggaatgc gcgtggggcc gtcccagacg gcaaaatcgg
     4801 aaacggtggc gcgactgggg agggcgaaaa cgtagttccc ttcttcgatg tggttcgtgt
     4861 gattggcaaa aacctgacgc acgaagacgc gagcatcgcc gttgtcgatg cggatggtga
     4921 tctccatctc ttcgagggaa agaatcgccg ggtcgggctg cgccttgtcg cgggggatca
     4981 ggacgccgcc gtcagcgagc agcaaggccg gcacaaggaa caacagcaag acgatgacgg
     5041 cgatgtgtgt tctcttcatg gctgcagcct ttgttcctga atcctgacca gtccgttgtc
     5101 tgtgccgacg tagatgtagc cgctgctctc cgcaagagcg gtcacgttgg gagagggcag
     5161 gccgccggtg atggtcgtcc agcgcgccga ttcgcggtcg tagacataca agcccttgcc
     5221 gagcgagcct gcgaagacgt ggcgcggagt caccagcatg gcattgggat tgacctcgaa
     5281 gggtgcggtt gccttatcga agggatgaaa atgacccgag cgatcgagcc cgaggacgcc
     5341 ggcgccgtag gtccccacca tccattcgtc cccgacgggg gccacagccg taatccagtt
     5401 gtgtttcagg ccggaggtgg cgccggcggt gtaattgacg cgcacagcgt ccttcccgaa
     5461 aaccgacaga ccgcccagcg tgccaatcat gagttcgtcc ccggagacgc ccagggcata
     5521 gacgtggtta ttcaccaggc cttgaaacgc gtagatactg cgagcgcccg aggcgtcgag
     5581 gaaagtcaat ccggcgggcg ttgccaccgc caacccgtcg cgatacagcg cgacatcggt
     5641 cacatggtcg gcgatgagcc cgtcggcgcg ggtcagcacc tgctcctggc ggccggagac
     5701 gccgaaacgt accaggccgt tggccgtggc gaccgccaca gtccccgtcc tggggtcggg
     5761 caagatcctg tttacgcaga agacgtggtc gttttccaca tgcgtggcgc gcggggcgcc
     5821 ggcttgcagc aggtcgaggc cgcggtcgaa atagccgatc cagagacggc cgctcgcgtc
     5881 cgcggcaagg gccgaaatgt tgcggtcgct gagaatggcc ggccccggct tcaagacctg
     5941 ttgccagcca agtccccgcg cagtcattcg atagagggcg tttcgggtga gcacataaac
     6001 gacctcgccc gactcaaaga actggcggac gtcggacaac ccggaagagg cattcgcgcc
     6061 agggccggag cgccgtttct cgagaggaac ggtgatgacg ccctggtttt cggagccgac
     6121 gatgaggttg gttggggtgg agaacaagga agtgacaagc accccgggcg caagcacatg
     6181 ggagaaccgt ccgcgatcga agaccgcaac tccaaggacg gtgccgacgt aagtcttgtc
     6241 tcccgaggtg gctacgcact gaacctgccg atcgggcaag ccctgttgtt cgttgaagga
     6301 ttccgtggca ccggcatgcc aatgaaggac tccctgattc agcgtgccaa tccacaaatc
     6361 cgattcggtg ccggcgaggg cggtgatgta cagattgctc aaggtgggat gcaaggccgc
     6421 gatccgcttg ccgtcgtaaa gcaggacgcc ccgcttcttc gtgccgatca agaggcggcc
     6481 ggcggccacc ggaagaatcg aggtgattgc gcgggcggcg gcatcctgag ggtagatctg
     6541 ccggaagctg cgtccgttga aggcgagcac gccgtcctgc gaagtagcca ccaccagttc
     6601 cggctcgcgg gaatccgcca ggaccgcggg agcgagagcg accaccagag atggcggaag
     6661 atcccggcct accgagaatt gccggagggg agtgcccccg aggtcgtatt cgaggaggcc
     6721 ggcggggccg gcaatgtaaa gctgatcctg aaagcgcgcc acctggagga agaccgctgg
     6781 cgcgctgacc gcctcaaaac ccaggtctgc cgggggcgcg aggggccgaa cgatgaagtg
     6841 aatttcctgc tcggaacgga cttcctgggt ggaaaggcgc agagcccgcg agacgcgcca
     6901 gccgacaaaa ccggcggcca gtacgatcag ggccagtccg cctgccgcaa ccgtgcgcca
     6961 tgacattctc cgtggccggc ccatagcccc ccttctagca gaacaacctg caaacccgaa
     7021 gtaaagatgt tgaaatctag agcaaatggg gcaccaaaat cttttgcctg gaaattcgct
     7081 agttttccgg acgtgcattc acgccactcg tgtatggcgg aacacactgt gtatgtccga
     7141 gcctggctgg acgcggcacc tagccaacac aatcagatac cagagtctca caactctctt
     7201 gagctacttg aactccgtga caatcattcg cgcagaggcg gcaggactgg tcgcgcaagc
     7261 aaccgtatta ccgtccacat ctgcggtttc cacgctgtgg cccgcaatga actgtgttcc
     7321 tttttgcacg gaagctcatt tccccagtgt ctaagatgtt cgtgcgtaac aatgtcgggc
     7381 aagtactgca gaacccggtg cgaggctatg gcacagactc cggacaccga ttgttcgtgc
     7441 ggacacgccg gctgcaaggg caaaacgtcc taattaagat aatgtgttga gcagttggtg
     7501 aaacctcgtc gtgagaaagg tcaagaccga aatgcccgct gtcctgacca ttgggcattc
     7561 gaaccgcaca tggaaggact tcctcaagct tctgcgggcg caccgcgtcg agcgcatcat
     7621 agacgtacgc agtatccccc ggtcgagaca caacccccag ttcaaccggg agaccctgtc
     7681 gacaaagctg cgggccgcga ggatcggcta cgtgtatttg cgaaagctcg ggggcttgcg
     7741 ccatgcacga cgcgattcgc cgaacatggg ctggcacaat acctcatttc gtggttttgc
     7801 tgattatatg cagacctccg aattcgaagc ggggttacac cgattgatga aattgtcggg
     7861 gcagaagcgg agcgcggtga tgtgtgcgga agcggttcca tggcgttgcc accgttcgct
     7921 tatcgccgat gcgctgactg tacgcgggat tcaggttgac gacattatga gcatgaaacg
     7981 ttcccaggtt cattcactca ttccttttgc acgcgtccaa ggccatcgca tcacgtaccc
     8041 tgccggcaat cgcggcggcg ggcgagtctc cccaattcgt tttcgagaga gttcacggaa
     8101 gaaccggaaa accaaggttt aagaatgtga tgaatgatgc ctcttgatta tcctttgtgg
     8161 ctgcgcgcgg cgcacttctt caattttctt ctcctgtcgc ttctcgttcg gagcggtctc
     8221 gagatcttga gcgcacatcc gagactttac tggaacgatc actgcacgcc gggcagcgaa
     8281 tggctcaagt tcacgaagaa gcagcgacct gaggaccggc tctggacggc gagtgacgag
     8341 gaaacctctt tcccgtccgc gatcgccctg ccgggccacg aaaatctggg tctcggacgg
     8401 cactggcact ttctcgcgga tttcgcgtgg ctgctgacag gtttgacata catcgtgatg
     8461 ctcttcgtca cgccggagtg gcggcggttg atcccaactt cctggcagat tgtcccggac
     8521 gcatggcacg caatgcttgg ctatcttagc ctccacatcg tggaaacccc aggaggatat
     8581 aacgcactac aacagttgag ctatgcggca gtgatattcc tgctcgcacc gttcagcatt
     8641 gcgaccgggg tggcgatgtc cccagccgtt gccgctcgtt ttccgtggta catcaaaatc
     8701 tttcacgggc ggcagggcgc acggagcctg catttcctcg ctctttgcac gttcatcgta
     8761 ttcttcatcg gacacgtcac aatcgtggct ctccatggtt ttcggagaga actggcgctg
     8821 attgttctag gacagacgca cgatccgcag ttgacccttg ctcttgtggt ctggctgtcc
     8881 gggttgaccg gcatagtaat cattcatacc gtgacaaccg tgtgttcgcg ccggcgcccg
     8941 cgctttgtcc agaaagccac tcagtcgatc accgatccgg tgcgcacact gcttttcggc
     9001 catgaaatct ccgcgcagca ttactcacat gcggacatct cgccctactt ctgggtgaat
     9061 ggccggccgc cgattgagga aacttttctg gcgatggcac gcgaaaactt tgcaaactat
     9121 agcctggaga tcggcggatt ggtcgaaagg caactgcatc taacgctggc ggacctccgc
     9181 gcaatgccga aacaaactca aattacaaag cattgctgca tccagggctg gtcaggcgtt
     9241 gcggagtggg gcggtgtatc tctgcatcac atcgtcgcac tttgccgtcc gcttcccgcc
     9301 gctcgctaca tcgttttcta cgcgttcgac aataaatcca catcggagcc gcacgcccaa
     9361 ggaccggggt acttctacgg cacgattcgc ttagagttgg cggaagatcc gcagacgatt
     9421 cttgcctacg aaatgaacgg ccagccgctg ccgatagtgc acggggcacc gctccggctt
     9481 cgcgtcgaaa cacagcttgg attcacgatg gtgaagtata tccgcgcaat cgagttcatc
     9541 gaggattaca aaaccatcgg ccagggccaa ggcggctggc gggaagatta ccagtatttc
     9601 agccaggagg cggggatctg atttaagagg tttggcagtt ccccgtgtga gccgtggcct
     9661 cttttagatt aaccctctga aattatctgg tttattccca cgaccaacgg ctgtttctgt
     9721 gcgtggctcg gctcgagaag gaacgtccca agaatattca tggcaaaggc cgtgatcccc
     9781 gccaattcga gcaccgccga gaccggtaac acagaccacg cccaattggc atagccttga
     9841 taggcgagaa cctcgctgga aacgcgaaga gcacatccaa tcgaaaggag tgcaagtccg
     9901 gcaaacatca tccgggtgct ccacaatagc cgcatccccg cgaacgcagg cagaatgcgc
     9961 tggcctacgc acatcaccat gacagcgatg aatccgaccg tcagcgcatg tcgagaggcg
    10021 ccccaaatcc ctccagagga atcccagagt gccgcgccca cgccaagcaa cgctgccccc
    10081 agcagccagc cgtaggccat gcgaacgaag aatgggaaac tccaatgcac gccccgtgtt
    10141 ttcggctcct gttgtgccgc ttcgaacatc cgcagcgccg cgatcaccag cgccgcgccc
    10201 acgacaaaga gcacagttgc ttcagccccc cgtcccacga gcgtcaaaac aacgcccgca
    10261 aagttcacca caagggcgct ccagagcagc ctgggtcgaa tcggcttgag accgaggaac
    10321 accggcatcc acttggtact gaatccccaa acgaacggca ccaggaaccc ccatgccacc
    10381 aacacgacat agcgctgatc gagaccgtgt ggccaggctg ggcttgcgcc ttgcaaggca
    10441 agataaatat tccccgcaag gttcgcgatg agcaccagca tcaaccctgt gcttgcgctc
    10501 acaacaaccc aaatccatgc atctcgtcgg tttttgccgg gatcttcagg ccggtgctgt
    10561 gaaaccgccc cgaagaaaat cacgaacgct gccagttcga ggacgcctga gagcggaagc
    10621 agcaaacgcc actgccaccc gtagacattc gcagcccagc gcgccgccac tccgattgtc
    10681 caaattgccc agcacgccca ggccgcgccg caggccggct tcgccgcgcc gcgtaacttc
    10741 ggaatcgaat agaaaccgat gccaaaaata aagctgccga tccagccgaa cacctgcgca
    10801 tggccgtgcg cctgcaacca tgccggcgaa acggatgcca cgctctcgcg tccgctgatc
    10861 tggaggagat tccacactcc aaggaacgtg cccgggagca acatgaacac gagccccgaa
    10921 ctgatgtaag ccatgaggag acgcgatagg gaggcctcac gcgcgcgcgc aagatccatt
    10981 acctcggcgg gagacagcga agcgcggcgc tcgtttttcg cggctgctac gggtgcttcg
    11041 agtggaatcg ccatgacgcc cccttaagca gccgcggctt ccatttccac cgcacgcgga
    11101 aagaggatgt tgttttccag atgcacatgc tggtgaagat ccgcttcgaa ttcccgcaag
    11161 tcctggtaca acgctcggta gctcgggcac gcatccgcag gggccgtgta ctccgaactg
    11221 gctttgcgaa tctgcttcag cagatctccg gcggaatcgt gctccttcat catggcgtga
    11281 atcggattcc gcaccgtctg aaaaaaggga ggctccagtg ttccgtttcc tttgacggac
    11341 ctttccagcg cctcgatgta ggggaacaag atctgctcct ctttctgcat gtgcatgatc
    11401 atttcctggc ctagaaccag aaagtgcttt tcgatgtccg cgatttcagg atgattctct
    11461 ccgtgctttg ctttcacttt cgccgccagg gctcgcacgc gcacgattcc ttcgcgaaca
    11521 tagcggtgat gcttttcccg gatatgctgc gttagttcgc ccagcggggc ggaaatccac
    11581 tgcgcatcgg ctggtcccac ctgttcactg ctttcgcgta gccgattaag aatttcctct
    11641 gaagacacat ccgcatgcgc gcatgcgtcg tgcagagact tgtctcctcc gcaacagtag
    11701 tccacgccag cttcctcaaa aatccgcgtc gctcccggat tcgcgagggc aatctctttc
    11761 actttggttt cgctattgaa attcatggtc gcctcctccc tacatcatca gtttggacga
    11821 agctcggctt gggggcagtg accaaagtca ctaccgcccg taagaatctg aaagtacagg
    11881 gattactcgg aagccgactg ttcgcgcttt aggcccgcga ggtccttcac tataatcctg
    11941 cgtccgtcca cttccagaaa cccttcggcg tggagtcggc tcagattccg tgagacgagt
    12001 tctcgcacgg tacccaattc cgcagccagg tcttgatggg tcttcgttag ttccacgtgg
    12061 acgccctctt tcgaagtcgt tccgctcgcc tgtgtcagcc gcaggatgag ggcgatcaat
    12121 cggtacctca ccgtcgtgaa cgacaagtct tcgatgatcc cgacgaggcg tcgcagccgt
    12181 gagccgacga ccgcaatcac tttgagcgct acctccggat gctcgcggca aaaattctga
    12241 aagtccttgc gtgagatgaa gagcacttcg gcgtcctcca acgcagacgc cgaggcagga
    12301 taatttcccc catcaaacac cggaagctcc gcgaaagaac tccccggttc ttccacggct
    12361 agaacttgtt cacgacccgc tggtgacagc ttgaaaatgc gaatcttccc tgtagccacc
    12421 aggaagagac ccgtgcacga gtcgccttcg caaaatagca gctcgccgcg ttgaaagtgc
    12481 ttcttactga ctcgcgtagc cagggcgtgc atctcttgct ccgtgagact cgcgaagagt
    12541 ggtgtcttcc gcaggaccat ttctatgttc tcgttcattg ccgcgctccg gccactactg
    12601 ctcttaccat aaccgaatat cccgatgaaa tggtcatgac gtgcgcgggg actcccagcg
    12661 tccgtgtctc gtcgcaaacg cgagctgagc gtctacgtcc gcatagagtg ccgatatcca
    12721 attccctgct ttcggtccag gcttgaaatg ctctagaata aagtcaatct ggcctgcgcc
    12781 cgtagctcag tcggaaagag cgtctgcctt ctaagcagag ggtcgggagt tcgagtctcc
    12841 ccgggcgcgc catattttcc cattcagatt aagattgaga tatcgaagtt aagccaagaa
    12901 gctacagttt gcgtggattc gccatgcaca ctgtccaata cggaacagtt cattccttgg
    12961 cgcggtgcca tgattcaggg catggcgcta acgctggtga cagcagtctt tgctccgctc
    13021 cctcacggtt tgtgaacagc ggcggagaaa acgcaaggga tttgtggtcc tggtcacgcc
    13081 acacatagaa acggggaaaa acgatgcagg ggcagtgcac gatgaattgc tgaaaccgcc
    13141 caatcaacat ttgagctacc atcgagccaa aatgcacgga ctaaggagag ttgataatgg
    13201 cctgcacaac tgttgtaaaa gatcctgtat gcggcatgga tatagaggct gctaccgccg
    13261 ctggacgaac cgagctcaaa gggcagatgt attacttctg cggctccaag tgcaaaggga
    13321 agttcgacct taatccggag aaatactcgg acaagtctga gggatctccg aagagcggcc
    13381 atggctgttg cagttaagtc ccaattgcca acacttacac tttggagttg gtctcagcag
    13441 ccgtatgagc gagtcaacgc aagtattcat acggttctga gagcaaaaca agccctgcga
    13501 aggccccggc ccagtgggtg agaagcaaga ccagtccatt cactcctcct tcaacgcttt
    13561 tttgtgggtc gattttcaag ggtcgcgagg agaatcttcc cagtctccaa ctgttagatt
    13621 ggaacgagga cagaacgatg gcgagatggc acaagaactt cttccttata ctacttttct
    13681 tcctcggtgg gggcgccggt cttgaagcgc agatggaccc ctatttcact gctatcaatc
    13741 atcctgttcc gcaggacacg atgatggtta tgctgctatc ggacttccag tccgcgcgat
    13801 ccaccaacga tttcttcacc ggcatgagca tggtgcagta cggcctcacc ccccgctgga
    13861 cggtggggtt catggcggag ggacagaaaa tcttcggttt gcccaccacc tacggcgggc
    13921 tgcggatcaa tagttacttt cgcgttttcc caaaggacca cctgctgaat ttcaccctct
    13981 acggcgagta cgaaggcctt aatggggcgg cgttgtacaa aatggaggtc gccggattcg
    14041 gcggcgaaga tttgaatgag ccactagcaa cggcgcggcg cacccacgtc cgcactttcg
    14101 agcagcgagc aatcatgtat cacgattggg ggcgggttaa ccttaccttt aacttcatca
    14161 acgagacgga cctggaaaac ggcgagaatg acttcggcta tgcctggggg gtgtttcggc
    14221 aggctgccta tacgacaatg gagacagaca aaggcatggc cggaatggcg gacaagtcga
    14281 aaatgaaagt gcctccggtg ttctcgtttc aacggctggg ctatgggttc gaaatgatag
    14341 gagcgctggg caacacacat cagttcggct tcgattggcc acgccagcag cagtatatgg
    14401 gtcctgtatt cagctattcc ctttctaagc gctggacggt tcaggtcgag ccggcatttg
    14461 gactctccca cgtgagcgat ccgtttgtat tgcgaatggg agttggatat tcgatcgacc
    14521 accttctaca tcggcgatca ccgactccgt gagcgacgtt gcaggctggt tctgtcccga
    14581 cggagttgaa acgaggcgtg gtcgggagtt caagtccccc cgggcgcgcc atctctttcc
    14641 attcagatta agattgcgat atcgaagtta agccaagaag ctgcaatttg cgtggattcg
    14701 ttttccgggg gggcaggtta ccttttcaca cttcgcgcac cggcgagagc gatgatatcc
    14761 taccgcccga accggttgtt ccggagggcg cgcgaagcga agagatttgc gcccccgccc
    14821 gagccagaag aaaaaagcag ccggaattgt taatgcgcgg gagctcatca accatgagga
    14881 agaccttcat ttacgcggcg gttgcagccc tgctgctcgc ggccggcacc gtgccagcct
    14941 gggcccaagc aagcacgcta atagttaaag gaaaggtcac gctgaatggc aaaccattcg
    15001 ccaatgcgca agttgtactc accaagccgg agactggaaa aacgcttaag acaaagactg
    15061 acaagaatgg tgagttcatt ctcgccggag tggaattggc cacgtatgaa gtcacagttc
    15121 tcggcaaaga tggtgaacag ttgtccaaga atgagacgac ctttgaggag ctacaccaca
    15181 aagagacaat tttgacgata gaaatcaatg ataccggagg agggagcggc gaaaagggtc
    15241 tgaccggccc gcccggccca cagcccaagc tctccaaaga gcagcgcgaa gaaatgaaag
    15301 cgcggcgtgc caagattgag cccctggtcg cgaaagtcca ggaagcatac gggcagaaga
    15361 actggctggc ggcggagacc gcgctgcgag agttgattgc agcggatcca gaacattggg
    15421 gcccctatca ggctttgggc aatacgcagt tcaacctggg caagtatcag gaggccgtac
    15481 aaacatacga aatcggcgta caaaaagcgc agaaaacttc gcccgatccc caagacccca
    15541 ccagtgatcc ttccaagatc aaggcaggaa tcggtcagat gctgacgcag cagggcaatg
    15601 cgtatctcaa gctgggaaaa accaatgaag cggtcgcggc cttcaccaaa gccgcgccga
    15661 tgagcgcaaa tccggcgatc gcctactaca atctgtgcgt cgttctctac aattcgggcc
    15721 agcttcagga tgttgggccc gcttgcgaca aggccattgc ggcggatcca aagcgtccgg
    15781 acgcatattt catcaagggc agagccctgt acgaaaattc ggagacggat gccagcggca
    15841 agcacctggc gcccgcgggg acgatcgaag cgctgaagaa gtacctcgag cttctcccgg
    15901 atggccctca tgccaccgag gcgaagctga tgctggaggg aattgccgcc cccaattgag
    15961 ctttcgacgg gcggcggcgg aattcctgct cgcaggcgcc atcggcaatc ttttcgccgt
    16021 tgccgagcgg tatcccgcgc tccgcgccgt ggaaagcgtg ctactgctgc aggaacagct
    16081 cacctccccg gaaaaccgca tcgcctacgc gcggcagttc tatgacgacg aggtgatgaa
    16141 gtacaacacc tggctctcca cctttcctca caatctcatt gcccgaggct tggggtttgc
    16201 tcccgcggtt atgttcaccg ccgaagaagc ggatctggtc agcccgcaag taaagctctg
    16261 agcctcttgt ccggcaaaca gccggcaccg ggcacgtcgc cggcttggct tgcctgcaaa
    16321 aagttggaac ggcactaatc ggctggctga gcctgcacag gctgcccgac gccggagcgg
    16381 cggagcgaag tgcgcagccg gtcgaggtag agatagacaa ccggggtcgt gaagagcgtg
    16441 agcatctggc tgaatatcag cccgcccaca atcgtgatgc cgaggggttg gcgcagttcc
    16501 gagccggtcc cgcgtcccag agccagaggc agcgctccca ggagagcggc catggtggtc
    16561 atcataatgg ggcgaaaacg cagcaggcat gcctgataaa ccgcgtccac cggcgacttc
    16621 ccatccactc gctccgcctg caaggcgaaa tcgatcatga gaatcgcgtt tttcttgacg
    16681 agcccgatca gtagaatgat gccgatgagc gcgatgacgt tgaggtccgt cttgaacagg
    16741 aaaagagcga gtagcgcgcc gactcccgcc gaaggcagcg tggagaggat cgtgatgggg
    16801 tggatgtaac tctcgtacaa gacaccgaga acgatataga ccgcggccag cgccgtgagg
    16861 atgagaacag gctgcgagga aagggaatcc tggaatgcct gcgccgtccc ctgaaaactg
    16921 ccgtggacat tcgcgggcat tccgatttcg cgctgggcgg cctgaatttg cgttacaacc
    16981 ggcccgaggg aagctcccgg ggcgagattg aaagaaagcg tgatggaagg aatctgcccc
    17041 tggtggttga ccgtcaaggg gctgctggaa tgttcgaagt gcgcgaaggc gctgagcggg
    17101 atgagctttc cggtcgtgga cgcgacgtaa atgacattca gtccctcggg attctgctgg
    17161 tattcgggga gaacctcgag aacgacgtga tactggttca gccccatata gatattcgaa
    17221 acttgacgct ggccgaacgc gtcgttgagg gcgttatcca ctgcttggaa cgaaacgccg
    17281 agccgcgaag ccgtggtgcg atccacaacc aactgagcct gtagcccgtg gatctgctgg
    17341 tcgctgttga tgtcccgcag ggccgggatg cggctcatgc gtttcatcat gaccggagcc
    17401 cattggttga gatcggcgat ctggtcggcc tggatcgtat attgatactg cgcgccgccg
    17461 aatcgcccgc ccacgctcaa atcctgaacg gcttgcagga agagcgtggc tcccgggatt
    17521 cttgcaagtt tgccgcgcag ccgcgcgatc acctggtcgg cgctgatctt ccgctcctgg
    17581 atcggcttga gctgcatgaa catgcgggcc gaatttgtgc cgccgctgcc gccggtgaag
    17641 gcggtaaccg tgtctacagc cggatcctcc atgatgatct tgacgtacgc tctcatcttt
    17701 tcggagacgg cgctgaagga gatgtcctga tcggcctgga caaacccgct gatgcggccc
    17761 gtgtcctgct gggggaaaaa acccttgggg acgatccaga agagataaat gtttacgccg
    17821 atcgtcgcga aggtgatgaa taggaccagc agttgatggc gcaagaccca gcgaaggccg
    17881 atatcgtagg tgtgcaacag gccgtcgaaa ctccgctcgg caacgcggta cgttgctcca
    17941 tgctttcccg tgtcgtggcg caggaagcgc gcgcacatca tcggcgtggc
//
LOCUS       DASYVO010000486        19033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_251525, whole genome shotgun sequence.
ACCESSION   DASYVO010000486 DASYVO010000000
VERSION     DASYVO010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 19033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19033
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251525"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /gene="larE"
                     /locus_tag="VGR03_16790"
     CDS             <1..857
                     /gene="larE"
                     /locus_tag="VGR03_16790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEV1995991.1"
                     /translation="GAAVSAEEKENLLLARLAEIPSLIVALSGGADSAYLAWAAHEAL
                     DTRALSITALSQSYSAHDRAIVDEFVSKLGVRHEFIETHEMDNPAYRANAADRCYFCK
                     DELFSALDELAHARGFAAVAYGVNADDTLDLRPGHRAATEHQVLTPLLDAGLSKAEIR
                     LLSQRAGLPTWDRPASACLASRLPYGTEVTPERLGLVERGEAALRALGFRQFRVRLHE
                     NLARVEISPDEMPRALAPEMAATIADRLKAAGFTYVALDLEGYRQGSLNETLSPVGQR
                     SDKAARGT"
     gene            926..1909
                     /locus_tag="VGR03_16795"
     CDS             926..1909
                     /locus_tag="VGR03_16795"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEV1995992.1"
                     /translation="MARYRNLYLPLVFFSAIVVLAGCHSGPSVSGTFDRNYDVTGPIR
                     LELTNASGDVDITGSVDGKVHVRGEVRVSGFGSERSQKRLDDTLANPPIEQRGDTIRI
                     GKEMSRMRNISIAYTIQVPHDTEVSATLASGAQTIRGVRGPVKAQAASGSIRVEKIER
                     DAQLTTASGSVSATDIGNDVRVSSASGSVTVSNARGDVRVKAVSGDIRVSNPGGRIEA
                     DTASGEVEIQGVASDVKAKAVSGRVSVQGNPGANSYWELKTLSGSLQLGVPASANLHL
                     SAEAVSGEIRTDIPIVVEEQGKHSLRAHMGSGGGRVDVHTVSGEIRVSGTK"
     gene            1997..3361
                     /locus_tag="VGR03_16800"
     CDS             1997..3361
                     /locus_tag="VGR03_16800"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV1995993.1"
                     /translation="MASQLFSTCVITILFLCAVAPPACGQAPAQDERSQNPSSKAAAS
                     KQAAPAKQPSPEEQLQQAISKAGNDRAALVRNLEAFLKEYPDSRQRPQIYRALVEASL
                     QLRDNMRAANYAERIVALSPDDISMTILTIQLLERNGDEAALRRATSYATRMLEFVER
                     SSSEEKSQKISREEWALEKKRDRMSVLTMRGRLELKLKDTVAARKDFEASYIILPSAT
                     AAEQLGEIAEMKKDSNGAIQQYSRAFALADATNGTASRREIRQKLGNVWRLAHGSDEG
                     LGEYLFRTYDEISQASVAAKPRKNADAREPADFTLRKAPEGTAFSLKDTKGRVLVVNF
                     WATWCGPCHALEPLFARVAADFQANPEVLFLAANCDEDESLVKSYLEEDKPRTPVVFA
                     DGLDRFFTVNSFPTVMVIDRVGKIAYRSDGFEPDTFEPNLAAAIRRALSPPSVPPPAG
                     NPAP"
     gene            complement(3374..4318)
                     /locus_tag="VGR03_16805"
     CDS             complement(3374..4318)
                     /locus_tag="VGR03_16805"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEV1995994.1"
                     /translation="MTVLLPFKLVYHERYDLNLGPHVFPSQKFRLIHEMLLREGIATP
                     EDFLRPNPASDEDMLRVHTPDWVRKLKTGTLTASEVMLLEVPYSPELVEAVWLAAGGT
                     ILAAQSALRDGFGANLSGGFHHAHPGHGEGFCAIHDVAVAIRRLQADGAVKKAIVVDT
                     DVHHGNGTSTIFRNDPTVFTISIHQENNYPAHKPPSSIDLNMADRADDDEYLGALIPA
                     VQKALDEFQPDILFYVGGADPYCEDQLGGLSLTKEGLKQRDRQVFEEARRRRIPVATT
                     LAGGYALRIGDTIRIHVNTIVAAQEVAEKHPQSSSMRG"
     gene            complement(4379..5308)
                     /locus_tag="VGR03_16810"
     CDS             complement(4379..5308)
                     /locus_tag="VGR03_16810"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV1995995.1"
                     /translation="MVEEELARQSDAAFPPVSEITAYLLGGGGKRMRPALLLLSASYA
                     GRKDRSAIRLAAVVELLHSATLIHDDVIDSADTRRGRPSANSKWGNHRSVLTGDWLYM
                     QSFQMALEERNFRILDILIDLTQKMVEGELIQLSKIGRIDVTEEDALRLATYKTACLF
                     SGCARLGAVLGGLEGKEEQALADYGRYAGLAFQLVDDLLDFTASAQQLGKPVLSDLKE
                     GKVTLPLIYAMENGHRDARELVARVLEEKEFNSVRPETIVSLVHESGALGRARALAQD
                     YACRAKACLNGGANSEFARALATLPDFILEREN"
     gene            complement(5378..6199)
                     /locus_tag="VGR03_16815"
     CDS             complement(5378..6199)
                     /locus_tag="VGR03_16815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV1995996.1"
                     /translation="MELIDSHAHIDFPQFAEDRDAMLDRARAAGVSTLLAIGTGPGPE
                     KLDSALPFAEQHDWIYATVGIHPHEAKEVTPQHLDDLARLAKHPKVIAWGEIGLDYYY
                     DHSPRDVQHRVFRDQMALAQQAKLPIIIHCRDGWSDCLSMIDQHWRPTGLGGILHCFT
                     STLEDARRGIEMGFLVSFAGNLTYPKTQNIRDVAKELPLEKILIETDSPYLAPQPWRG
                     KRNEPAYVAEVARTLASVRNLSPDEVAAATSENFRRFFGLARPATLAAPGFRDKG"
     gene            complement(6349..8334)
                     /gene="metG"
                     /locus_tag="VGR03_16820"
     CDS             complement(6349..8334)
                     /gene="metG"
                     /locus_tag="VGR03_16820"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV1995997.1"
                     /translation="MPDKPKYYLTTPIYYTNGLPHIGHTYSTIVCDTIRRYKRMRGYD
                     VVMTTGTDEHGVNVERSAKKAGVPESEFVAKMAAEWKGLWDELGVQGDEFIRTTDLKH
                     AHTVQWLFKLCRENGFVYPGHYTGQYCIHDNAYVDVKPGENCPDCNRPTETVTEANFF
                     FKLSAFQKPLLDWYAKHPDFIQPEARRNEVLSFVEGGLRDLSITRTSIRWGIPVPGEE
                     PHVFYVWFDALTAYLSAVGGPQYEKTGFWPADVHLVGKEIIRFHAVYWPAFLMAAGLP
                     LPKQIWAHGWLLMDSTKMSKSLGNVVRPRPIVHVLGMDALRYYLLRETVFGQDGNFSY
                     DALVQRYNSDLANGLGNLSSRVLTMIEQHLMGSTPAKPSAAEDARDEQVNKLRLDTIL
                     QTCEHYDRLEFSRALETIWRLIAEVDKYLVETKPWVSVKTPGSFDQFVTTMYTAYQAL
                     REIVILAHPILPDATKGIWKQIGQTDDLGSVDIHLDWPAIASFRIGKIEATFPRIEKA
                     EAIERMESMENEAQKQPSPVTAAPTPASTTAASSATAAAPQAAPEKIVIDDFTKVEMR
                     VGQIKTAERIVGADKLLKLTVDIGTEIRQICAGIAQFYEPEALIGRKVAVVVNLAPRK
                     LRGVESNGMIVAASVGPEGRPVLAGFLEDVEVGSRLK"
     gene            complement(8445..9623)
                     /gene="larC"
                     /locus_tag="VGR03_16825"
     CDS             complement(8445..9623)
                     /gene="larC"
                     /locus_tag="VGR03_16825"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEV1995998.1"
                     /translation="MKLAYFDCFSGISGDMTLGALVDAGCDVEHLRAELRGLQIPGWE
                     LTAEKVWKNGMAATYVKVKTEDQQKHRSLSAILEILQKSQIAPQVRERAAAIFTKLGE
                     AEARVHEVPLEKIHFHEVGAVDAIVDIVGACIGFHALGIEKFACSALNVGGGTAKMAH
                     GVLPVPAPATANLLQGKPTYSNGVQKELVTPTGAAIVATLCDAFGPQPPMSVTAIGYG
                     AGTADLEGQPNVVRIMIGEAAEKAVAGFDEEIAVIEANLDDMNPQIYGYFLEKALAAG
                     ALDVYTTPVQMKKNRPGTLLTVLCKPQDTNALMSLIFAETTTFGARTYRAQRRTLPRE
                     FLNVATAFGDVRIKISRVNGRILHVTPEYDDCRKLAVEKNVPLQRVISEALRAYESTV
                     "
     gene            10175..12328
                     /gene="argS"
                     /locus_tag="VGR03_16830"
     CDS             10175..12328
                     /gene="argS"
                     /locus_tag="VGR03_16830"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV1995999.1"
                     /translation="MYQRLTERLREALAGHIREKYGVELAIVLERPPKIEMGEAASPV
                     CFELAKRLKRAPRQIAQEIANGLGVVEGIAKVEVAGGGYLNAYFDREAFWEGVKAESG
                     EENGERTQRRDAPTGSGQAPDAEKNGKRQKAVAAGGENGKIIVEHTSINPNKAAHIGH
                     VRNAVLGDTMVRILRHAGNRVQIQNYIDNTGVQVADVVIGFLHMEQRTPVGVKMMAME
                     PKFDYHCWDLYAKATNFLAEDKARAAKLRGETLKSIEEGKGEDAEVAQVVADAIVGCH
                     LRTMARLDIGYELLARESEILHLKFWDAAFEMLKKSGAIQLATSGKMAGCWIMPWKDE
                     EHSRINAEGTGNTEDAEKTEEDENSQDKIIVRSNGTVTYVGKDIAYQLWKFGLLGKDF
                     HYRKWPTPPQAADEKDAPEGQTVWATTSGANDTEAPHFGEPASSVYNVIDARQAYLQK
                     VVAAGLRALGHAKAAEQSIHLSYEIVALTPRCAAEMGYELTPEEAKKPHVEVSGRKGF
                     GVKADDLLDKLEAATLAEVKKRHPSHHPAVPDLPSYATVEQHQIAHAIAVGALRFFLL
                     KFTRNAIIAFDFKDALSFEGETGPYCQYAVVRIRGIRRKGAEAGATNVEVTKESVSQL
                     LAGAEENSLWELLVAAGSLDYAVDAAIATQEPAFMAKYAFQLAQAFNNFYHKHHILSE
                     ADEQKQAFLLRLTELVEAQLVRALSLLGIESPEKM"
     gene            12347..13141
                     /locus_tag="VGR03_16835"
     CDS             12347..13141
                     /locus_tag="VGR03_16835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1996000.1"
                     /translation="MKTLPPDLEEVIGKALQKSQSVPGFLGDNEARFLALLAACTPAS
                     GVIVEIGSFKGKSTVLLASVAERYGLGPVVAIDPHTAPSVTDPKIAPGSSTYEEFVGA
                     LRSTQLEEQVEIRRVYSREAAKGWNRPIRLLWIDGDHTYEGAREDFELFSPYLAKGAI
                     VAMHDALHAYEGPIRVFVERILRSDQFGPTGFVQSVAWGQFRPEGGATFRMQREKLES
                     RAVRLLPYLDKKRPFRGFTKLGYKLARSRVPRDAISPVSWSDLVSG"
     gene            complement(13168..14658)
                     /locus_tag="VGR03_16840"
     CDS             complement(13168..14658)
                     /locus_tag="VGR03_16840"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEV1996001.1"
                     /translation="MQIMLETKPFATLETDALVSYVFEEADPAQGRIAEIDQAAAGLL
                     RKLAKSGELTGKLLEFTLIHAPAGLKAARLLLVGAGKREQFNAAALRKIAGAALRYLK
                     ARSVKNFALLVREGNATGEIAQAIAEGALTANFETDKYKTDKKNDKNIESVQIAGFSD
                     AERAAGDKGLSKGRIIADAQNFARDLVNEPSNKLTPRILAEKAEAMAKEAGLTVEILD
                     EKKIADLKMGALLSVAQGGPEPPRVMVVTYTPANLKPGAPVIGLVGKAVTFDTGGISI
                     KPADGMEKMKYDMAGGATMLGVMRALAALKPSVKVICVVPSTENMPGGKAQKPGDIQT
                     AMSGKTIEVLNTDAEGRLILADGIHYAKQLGATHLVDAATLTGAIVVALANVNVGVFG
                     SDQPFTDKLLASSKAVGEKMWQMPIDDDYRDFIKGSFADIQNISSGKGGGAITGAMFI
                     KEFTGDTPWIHLDIAGTAWNDDAKPWLAKGPTGVALRTLVHLVLSY"
     gene            complement(14665..15162)
                     /gene="smpB"
                     /locus_tag="VGR03_16845"
     CDS             complement(14665..15162)
                     /gene="smpB"
                     /locus_tag="VGR03_16845"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV1996002.1"
                     /translation="MAKPAKKTEKSAKSGEQIVAQNRAASYNYLILEKHEAGLVLHGT
                     EVKALREGKANIRDAYVDFKSNSAWLVNAHIAQYLPGGPWNHEPLGSRKLLLHKREID
                     KLSGRTESKGLTVIPLKIYFRNGIAKVEIALAQGKKSWDRRQDERTKEARREVEQSMY
                     NNRRR"
     gene            15314..17608
                     /locus_tag="VGR03_16850"
     CDS             15314..17608
                     /locus_tag="VGR03_16850"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HEV1996003.1"
                     /translation="MRRWGSLFLCVGFGLLAAGCPKGQPDFNQGKKAETIQDYDAAFV
                     FYQKAAKADPYNSSYKIKLNQIRFEASQQHVKAGVELRKKGDLQGAASEFQRAQAIDP
                     ASPVADQELHKTVGMIAEKNRAADAAAEPPPDPNDQPLAEMPPEIKPLSRAPINYKAS
                     NDAKIVFDTIGKLAGLTVIYDPDFPARRISVDLNNVTLEQALEIVSLESKAFVKPVTE
                     NIIFVIPDQPQKRRDYEEQVVRTFYLSNTVQPQDLTEIVTGLRQLLDLKRIQQLNSQN
                     AIIVRDTPDKLLLAEKIIRDIDKAKPEVVIQVEVLEARTDRLRDLGILPGQTASIAIN
                     PNGTASTPTGGTPATPNSGITLSQLRHLNGGAYAVTLPSLTANAVLTDTNTRIIQNPE
                     IRSLDGQTAKLRIGDRIPIATGSFSSGIGGVAGGVAGGISPLVNTQFTYLDVGVNIDL
                     TPRVHPNRDVSLKLKVEVSSHTGDQSIGGITQPIISQRVIEDDIRLKEGEVNIVGGLV
                     QRTESKTIEGWPGLAKLPLLRYLFSHNKTDHQEDEVLIVLTPRIVRIPEWTKANLRPL
                     YSGSETNVQVKRESEIRAPVQQPPPAQAQPPASNQNMGAATPAPAPGAAAPAAGAIQG
                     APAAKLRFEPQSLSLKAGQTATIGVVVDNVSDLFSIPMLLQYNPAVISVEEVQHGGFL
                     SGGTQEIAIVYQPFKDKGQAIISATRRPNTPGVSGSGTLIGIVVKALAPGSSNLSIVQ
                     VNAKDSQQKLIPLITSEATLQVQP"
     gene            17655..18137
                     /locus_tag="VGR03_16855"
     CDS             17655..18137
                     /locus_tag="VGR03_16855"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV1996004.1"
                     /translation="MGRRIQRRPFSSETGMTLLELIIACAILLILSSAALPIAKYTII
                     RQKEAELRRDLREMKDAIDRYKDAADRNQIRVEIGSEGYPPDLETLVKGVQLGASSDK
                     KIRFLRKIPVDPMTGRAEWGLRAVQDDPDSTSWGGKNVFDVYSKSSGTALDGTRYSTW
                     "
     gene            18156..18602
                     /locus_tag="VGR03_16860"
     CDS             18156..18602
                     /locus_tag="VGR03_16860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEV1996005.1"
                     /translation="MKRLLQTRNGRHSLRKRPGRLFETGFTLIELMIVITIIFILVGI
                     AAVRFDKSVQRAKEATLKQDLQAMRQSIDNYTLDKQAAPQSLEDLVQVGYLHRVPTDP
                     MTGAADWVPQFDSVVLSPEQSSTGMVDVHSNSARVSSFENTPYNEW"
BASE COUNT         4030 a         5215 c         5870 g         3918 t
ORIGIN      
        1 agggagcagc tgtgtccgca gaagaaaagg aaaatcttct gctcgctcgg ctcgccgaga
       61 ttccttcgct catcgtcgcg ctctcgggcg gcgcggactc cgcctacctg gcttgggcag
      121 cccacgaagc gctggacacc cgcgccctat ccattacggc actctcgcag agttactccg
      181 cccacgatcg cgccatagtg gatgagtttg tgagcaagct gggcgtgcgc cacgaattca
      241 tcgaaacgca cgaaatggat aaccccgcct accgcgcgaa cgcagccgac cgttgctatt
      301 tctgcaaaga cgagctgttt tccgcgttgg acgaattagc gcacgcgcgc ggttttgccg
      361 ccgtcgccta tggcgtgaat gccgatgaca cgctcgacct tcgccccggc catcgcgccg
      421 ccacggagca tcaggttctt acgccgctgc tcgacgccgg cctgagcaaa gccgagattc
      481 ggctgctctc gcagcgtgcc ggattgccca cctgggaccg ccccgcctcc gcttgcctcg
      541 cttcgcgctt gccctacggc acggaagtga caccggagcg tctcggactt gtcgagcgcg
      601 gcgaagcagc gctgcgcgcg cttggattcc gccaattccg cgtgcggctc cacgaaaacc
      661 ttgcgcgcgt ggaaatatcg cccgacgaaa tgcctcgcgc acttgcgcca gaaatggcag
      721 ccaccattgc ggaccggctg aaagccgcag gcttcactta cgttgccctg gaccttgagg
      781 gatatcgcca aggctctctt aacgagactc tttcgcccgt aggccagcgt tctgacaagg
      841 ctgctcgggg aacataattc cctgctcttg cgtagctata agtggcacgg ctcagagcaa
      901 gtctctgcac cgttacggag gatgaatggc gcgctaccgc aatctttact taccgctcgt
      961 gttcttttcg gccatcgttg ttctggcagg ctgccatagc ggaccttcgg ttagcggcac
     1021 cttcgatcga aactatgacg tgacgggtcc gattcggctg gaactcacca acgcctccgg
     1081 tgacgtggac atcaccggca gcgtcgacgg caaagtgcac gtgcgtggcg aagtgcgcgt
     1141 atccggattc ggctcggaac gctcgcagaa acgcctggat gataccctcg caaatccccc
     1201 catcgaacaa agaggcgaca ccattcgcat cggcaaggaa atgtctcgca tgcgcaacat
     1261 cagcatcgcc tacacgattc aggtgccgca cgacacggaa gtcagcgcca ccctggcgtc
     1321 cggcgcgcag acgattcgcg gcgtgcgcgg cccggtaaaa gcgcaggctg cttccggatc
     1381 catccgtgtg gaaaaaatcg agcgcgacgc ccagcttacg accgccagcg ggtcggtgtc
     1441 ggccactgat attggcaatg acgtccgcgt atcatccgct tcgggaagtg tgacggtctc
     1501 gaacgctcgg ggcgatgtgc gcgtgaaggc cgtttcgggc gacattcgtg tatccaatcc
     1561 cggggggcgt atcgaagcgg ataccgccag tggcgaagtc gagattcagg gtgtggcaag
     1621 tgacgtgaag gcgaaagcgg tctcggggcg cgtttccgtc cagggaaatc caggcgccaa
     1681 cagctactgg gaactgaaaa cgctttcggg ttccctgcaa ctcggcgtcc cggcttctgc
     1741 aaatctccat ctctcggcgg aagcggtctc cggcgagatt cgtaccgaca ttccgatcgt
     1801 tgttgaggaa caaggcaagc attctcttcg agctcacatg ggcagcggtg gcgggcgcgt
     1861 ggacgtgcac acggtctcgg gcgaaattcg cgtgagcggc acgaaatagc atcgcgtaca
     1921 ttcattttgc agatttcgca gaaaacattt aagaactagc gacaattctt cccgatacct
     1981 ttagaatccc gcttgtatgg cttcccagct cttctcgact tgcgtgatta caattctttt
     2041 tctttgcgcg gtggctccgc cagcctgcgg tcaggccccc gcacaagacg aaaggagtca
     2101 gaacccatcg tcgaaagccg ccgcctccaa gcaagccgct cccgccaagc aaccttcgcc
     2161 cgaagaacaa ttgcagcaag ccatcagcaa agctgggaac gaccgcgcgg cgctggtccg
     2221 taatttggag gccttcttaa aggagtatcc ggattcgcgg cagcgtcctc agatttatcg
     2281 cgcgcttgtg gaagccagct tgcaattgcg ggacaacatg cgcgccgcca attacgccga
     2341 gcgaatcgtc gcactcagcc ccgacgatat ctccatgacc atcctgacga ttcaactgct
     2401 cgaacggaac ggcgacgaag ccgctctccg ccgcgccaca agctatgcca cccggatgct
     2461 cgaatttgtc gagcgcagct cttccgagga aaaatcgcaa aagatttccc gggaagagtg
     2521 ggctttggaa aagaaacgcg atcgcatgtc cgtgttgacg atgcgcggaa gactcgaact
     2581 gaaattgaag gacacggttg cggcccggaa agacttcgag gcgagttaca tcatcctccc
     2641 cagcgcgacg gccgcggagc agctcggcga aattgccgag atgaaaaaag actcgaacgg
     2701 cgcgatccag caatactctc gcgccttcgc gcttgccgac gcaacgaatg gaaccgccag
     2761 ccgccgcgag atccggcaaa aactgggcaa tgtctggcgg cttgcgcatg gctccgatga
     2821 gggtctaggc gagtatctgt ttcgcaccta cgatgaaatc tcgcaggcct ccgtcgcggc
     2881 caagccaagg aaaaatgcgg acgcccgcga acccgcggat ttcacgctta ggaaagctcc
     2941 cgaaggcacg gctttttcgc tgaaagacac gaagggaaga gttctcgttg tcaatttctg
     3001 ggccacctgg tgcggccctt gccacgcgct tgaaccttta ttcgctcgcg tcgccgcgga
     3061 cttccaggcg aatccggaag tactttttct ggccgccaac tgtgacgaag acgaatctct
     3121 ggtgaagagt tacttggaag aggacaaacc gcgaacgccg gttgtatttg ccgacggcct
     3181 cgaccgcttc ttcacggtca actcctttcc gaccgtgatg gtgatcgatc gcgttggaaa
     3241 aattgcctat cgctcggatg gtttcgagcc cgataccttt gagcccaacc tggcagcggc
     3301 catccgccgc gcgctgtctc cgccaagcgt tccgccacct gccggaaatc ccgcgcccta
     3361 accgcgcaga gccctaacca cgcatcgatg aactttgcgg atgtttctcc gccacttcct
     3421 gagcggccac aattgtattg acgtggattc gaatggtgtc tccgatgcgg agtgcatagc
     3481 ctccggcgag agtcgtcgcg accggaattc tgcggcggcg ggcttcctcg aaaacctgcc
     3541 ggtcgcgttg cttcaatcct tctttcgtta gtgacaggcc accgagctgg tcctcgcagt
     3601 aggggtccgc gccaccaaca taaaagagaa tatcgggctg aaattcgtcg agcgccttct
     3661 gcaccgctgg aatcagcgcg cccagatatt catcgtcgtc ggcgcggtcg gccatgttga
     3721 gatcgatgct tgacggcggc ttgtgcgccg gatagttgtt ttcctgatga atcgaaatcg
     3781 tgaagaccgt cggatcgttc cggaagatcg tggaggtccc gttcccgtga tgcacgtccg
     3841 tatcgacaac gattgctttt ttcaccgcgc cgtccgcctg cagccttcga atcgccaccg
     3901 ccacatcatg aatcgcgcag aaaccttcgc catgccccgg atgcgcgtga tgaaatcctc
     3961 cgctgagatt cgcaccgaat ccgtcgcgca gcgctgattg cgccgcgaga atcgttccac
     4021 cagcggcgag ccacaccgct tcgacaagtt ccggcgaata cggcacttcc agtaacatca
     4081 cttcactggc agtgagcgtg ccggtcttca gtttgcgtac ccagtccggc gtgtgaacgc
     4141 gcagcatgtc ctcgtcgctc gcaggattgg gccgcaaaaa atcttccggt gtggcgatgc
     4201 cttcgcggag cagcatttca tggatgaggc gaaatttctg cgatgggaaa acgtgcgggc
     4261 cgagattcaa atcatagcgc tcgtgataca cgagcttgaa tggaagaaga actgtcatca
     4321 cccggaatca gaatttctct tctcacttcg aactgccaac tatccactga aactatcctc
     4381 agttttcgcg ttcgagaatg aaatccggga gcgtggcgag ggcgcgcgca aattcggagt
     4441 tagcaccgcc attgaggcac gccttggcac ggcaggcata gtcttgcgcg agcgcgcggg
     4501 cgcggccgag cgcgccggat tcgtgaacca gcgaaacaat tgtttcgggt cgaacggaat
     4561 taaattcctt ttcttccagc acacgcgcga ccagctcacg cgcatcgcgg tggccatttt
     4621 ccatcgcgta gatcaacggc agtgtgacct tgccttcttt caaatcgctg aggactggtt
     4681 tgccgagctg ttgcgctgac gcggtaaaat ccagcaaatc atcgacaagc tgaaacgcaa
     4741 ggccggcgta tcgtccgtaa tcggcgagcg cctgctcttc tttaccctcg aggccgccca
     4801 gcactgcgcc gaggcgcgcg cagccgctga aaagacatgc ggttttgtag gtggccagcc
     4861 gcagcgcatc ttcttcggtc acatcgatgc gcccgatttt cgagagttgg atcagttcgc
     4921 cttcgaccat tttctgagtc aagtcgatca gaatgtcgag aatgcgaaaa ttgcgttctt
     4981 ccagggccat ctgaaacgac tgcatgtaca gccagtcgcc ggtgaggacg ctgcgatgat
     5041 tgccccattt tgaattggcg gaagggcggc cgcggcgcgt gtcggcgctg tcgatgacgt
     5101 cgtcgtggat caacgtcgcg ctgtgcaaaa gctcgacgac ggcggcgaga cgaatggcgc
     5161 tgcgatcctt gcggcccgca tagcttgcgg aaagaagcaa gagcgcggga cgcattcgct
     5221 tgcctccgcc tcccagcaaa taggccgtga tttcagagac gggcggaaag gcggcgtcgc
     5281 tttgtcgcgc gagctcttct tcgaccaaga cgaggtcgtc gcgaacgaga tcaaaaatct
     5341 ctttggccgt gagcagcgcg atggcaaatc tcctgattta acccttgtct ctgaaacctg
     5401 gggccgcgag agttgccggc cgagcaagac cgaagaagcg ccgaaaattc tcggaggtgg
     5461 cggcggcaac ttcgtccggc gacaagtttc tcacactcgc cagcgtgcgc gctacttcgg
     5521 caacgtaggc aggctcgttg cgcttgccgc gccagggctg cggcgcaaga tacggagagt
     5581 ccgtctcaat caaaatcttc tcaagcggca attctttcgc aacgtcgcga atgttctgtg
     5641 ttttcggata ggttaaattg cccgcaaagg aaaccaggaa acccatttcg attccacgcc
     5701 gcgcatcttc aagtgtgctg gtgaaacagt gcagaatccc gccgaggcca gtgggccgcc
     5761 agtgttggtc gatcatgctc aggcaatcgg accagccatc gcggcagtga atgataatgg
     5821 gaagcttcgc ctgctgcgca agggccatct ggtcgcggaa gacgcgatgc tgcacgtcgc
     5881 gcggagaatg atcgtagtag tagtcgaggc cgatctcgcc ccaagcaata actttgggat
     5941 gtttcgctaa ccgggccagg tcatccaaat gttgcggcgt tacttccttc gcttcgtggg
     6001 gatgtatgcc gacggtggcg tagatccaat catgctgttc ggcaaatggc agcgcggaat
     6061 cgagcttctc cgggccgggg cctgtgccga tggcgaggag tgtcgaaaca cccgcagcac
     6121 gcgcgcggtc gagcatggcg tcgcggtctt ccgcgaactg cgggaaatcg atgtgtgcgt
     6181 gcgagtcgat gagttccata aagctatgat ttgtgactcg tgcctagcgt ctggggaccg
     6241 ttccttcttc tcttttagtg gcacagacac tcttgcctgg gctcttggtt tgttctggtc
     6301 aaagcagaag cacacagcca agaatggctg tgccacacaa acctggacct atttcaggcg
     6361 cgagccgact tcgacgtctt cgaggaagcc ggcgaggacg gggcggcctt ccgggccgac
     6421 ggaggcggcg acgatcatgc cgttggattc gacgccgcgg agcttgcgag gcgcgagatt
     6481 cacgacgacg gcgactttgc ggccgatgag cgcttcgggt tcgtagaatt gcgcgatgcc
     6541 tgcgcaaatc tggcggatct ccgtgccaat atcgacggtg agcttcagca acttgtctgc
     6601 gccgacgatg cgttcggcgg tcttgatttg cccgacgcgc atctcgactt tggtgaagtc
     6661 atcgatgacg attttctccg gtgcagcttg tggcgcagcg gcagtagcgc tgctagcggc
     6721 ggtagtgctc gcgggcgtgg gcgcagccgt aacgggcgag ggttgctttt gtgcttcgtt
     6781 ttccatggat tccatcctct cgattgcttc tgccttctca attcgtggaa aagtagcctc
     6841 gatcttccca atgcgaaaac tcgcaatcgc cggccagtct agatggatgt caacactacc
     6901 taagtcatca gtttggccta tctgtttcca gatccccttg gttgcgtcgg gcagtatggg
     6961 gtgagccaaa atcacaattt ctcgaagcgc ctgataggcc gtgtacatag tggtgacaaa
     7021 ctggtcgaaa cttccgggag tcttgaccga cacccacggc ttagtctcca caagatattt
     7081 gtcgacttct gcaatgagac gccagattgt ctccaaagcc cgcgaaaact ctaggcggtc
     7141 atagtgctca caagtctgca gaattgtgtc cagtctcaac ttgttgactt gctcgtctct
     7201 cgcatcctct gccgccgatg gcttcgcggg tgtactgccc ataaggtgtt gctcgatcat
     7261 tgtgagcacg cggctggaga ggttgccaag accgttggcg aggtcggagt tgtagcgctg
     7321 gacgagtgcg tcgtaactga aattgccatc ctggccgaaa acggtttcgc gcagaagata
     7381 gtagcgcaag gcgtccatgc cgaggacatg cacgatgggc cgcgggcgaa cgacgttgcc
     7441 gagcgacttg ctcatcttgg tgctgtccat gagcagccag ccgtgagccc agatttgttt
     7501 cggcaacgga aggccggcgg ccatcaaaaa ggcgggccag taaacggcgt gaaagcgaat
     7561 gatttccttg ccgacgagat gaacatcggc gggccagaat ccagttttct catactgcgg
     7621 gccgccgacg gcgctgagat aggcggtgag cgcatcgaac cagacataaa agacgtgtgg
     7681 ctcttcgccg ggcacgggaa tgccccagcg aatcgaagtg cgcgtgatgc tgagatcccg
     7741 caggccgcct tcgacgaagc tgagaacttc gttgcggcga gcttcgggtt ggatgaaatc
     7801 gggatgcttg gcgtaccaat cgaggagcgg cttttgaaac gcagaaagtt tgaaaaagaa
     7861 atttgcttcg gtgacggttt ctgtgggacg gttgcaatcg gggcaatttt cgccgggttt
     7921 tacatccacg taggcattgt cgtggatgca gtattgcccg gtgtagtgac cggggtaaac
     7981 gaacccgttt tcgcggcaca gcttgaaaag ccattgcacg gtatgcgcgt gcttcaaatc
     8041 cgtagtgcgg ataaattcgt cgccttggac accgagttca tcccagagcc ctttccactc
     8101 cgcggccatt ttggcgacga attcagattc gggaactccg gctttcttag ccgagcgctc
     8161 gacgttcacg ccgtgttcgt cggtgccggt ggtcatgacc acgtcgtagc cgcgcatgcg
     8221 tttgtagcgg cggatggtat cgcacacgat ggtcgagtag gtgtggccga tgtgcggcag
     8281 gccgttcgtg tagtagatcg gcgtggtcaa gtaatatttg ggtttgtcgg gcatcgtcga
     8341 gtattcagtt ctcagttcta agtaagcaaa aactacaaac cctttatctt cgcgaaatct
     8401 cgccaagctg gggcgcagca atgctgcgcc cctacaaata aaattcagac agtcgactca
     8461 taggcgcgga gagcttcgct gatcacgcgc tgcagaggca cgtttttttc gacagctagt
     8521 ttgcggcagt cgtcgtattc gggtgtgacg tgcaagatgc gaccgttcac gcgggaaatc
     8581 ttgatgcgca catcaccgaa ggccgtggcg acgttgagga attcgcgcgg cagcgtgcgg
     8641 cgttgcgcgc gataggtgcg cgcgccaaag gtggttgttt cggcgaagat cagcgacatc
     8701 agcgcgttgg tatcctgcgg cttgcacaaa accgtgagca gcgtgccagg gcgatttttt
     8761 ttcatttgta caggcgtggt gtaaacgtcg agagcgcccg cggccaaggc cttttccagg
     8821 aagtagccgt agatttgcgg gttcatgtcg tcgagattgg cttcgatgac ggcgatttct
     8881 tcgtcgaagc ccgcgactgc tttttccgcg gcttcgccga tcatgatgcg aacgacgttg
     8941 ggctggccct cgagatccgc cgtgccggcg ccgtagccga tggcggtgac gctcattgga
     9001 ggctgcggac caaaggcatc gcaaagcgtc gcaacgatgg cagcaccagt gggcgtgaca
     9061 agttctttct gcacaccgtt ggaatacgtt ggtttgcctt gcagcaaatt cgccgtggcg
     9121 ggcgcgggga cgggcaaaac tccgtgcgcc atttttgcgg tgccgccacc gacatttagt
     9181 gcggagcagg cgaacttttc gatgcccaga gcgtgaaagc cgatgcaggc gccgacgatg
     9241 tccacgattg cgtccacggc gccaacttcg tgaaagtgaa ttttttcgag cggcacctcg
     9301 tgaacgcggg cctcggcttc gccaagtttc gtgaaaatcg cggcggcgcg ttcgcgcacc
     9361 tgcggtgcga tctgtgattt ctgcaagatt tcgagaatcg cgctgagcga gcgatgcttt
     9421 tgctggtctt cggtcttgac cttgacgtag gtggcggcca tgccattctt ccagactttt
     9481 tcggcggtga gttcccatcc agggatctgc agaccacgga gttcggcgcg gaggtgttcc
     9541 acgtcgcagc cagcgtcgac cagagcgccg agcgtcatgt caccgctaat gcccgaaaaa
     9601 caatcgaaat aggcgagttt catgggatgt cgccgccgaa cggtggaacg agcagatggg
     9661 aatgtcactc cgccggatgc agattgatac tatcggcgga ggcagagtgt gtcaaacgaa
     9721 ggagcgctcg gagggtcctc cgagcgggct gcagaattcg gaatgatgcc ccccccaccc
     9781 accccttttt tcataagtgt tcattctaaa ggaagttaaa gtcatttgtt ttgatacact
     9841 tttgcaagtg ttgattctaa aggtacttag attgcaccaa aattgtgcaa attcgcagca
     9901 aaatccaccc gctgtgccgt ggccagggga acagccgata gaagaaggca gaaacgctga
     9961 accaagcaaa aaaaaaacgg cccacgagtg gccgcaaact agctacacat ttaactaaca
    10021 aacatgataa cggtttagcc ggagacagtc aaggaaaaaa acgatatttc ggatagcttt
    10081 gccggacgcg aaccgggagg gacggctctg ggagactggt gttcctcagg gggagtctcc
    10141 tatactcgat ggccttggga aagaacggag agccatgtat cagagactga cggagcggtt
    10201 gcgcgaggcg ctggcggggc acatccgcga gaaatacggc gtggagctgg cgattgtgct
    10261 ggagcggccg ccgaagattg agatggggga ggcggcttcg ccggtgtgtt ttgaattggc
    10321 gaagcggttg aagcgggcgc cgaggcagat cgcgcaggag attgcgaatg gtttgggtgt
    10381 ggttgagggg atcgccaaag ttgaggttgc gggtggcggg tatttgaatg cgtattttga
    10441 tcgagaggcg ttttgggagg gtgtgaaagc ggaaagtggc gaggagaacg gagagagaac
    10501 tcaacgcaga gacgccccga ccgggtcggg gcaggcgccg gacgcagaga agaacggaaa
    10561 aagacagaag gctgtagcag cgggtggtga gaacggaaaa atcatcgtcg agcatacgag
    10621 catcaatccg aacaaggcgg cgcacatcgg gcacgtgcgg aatgcggtgc tcggcgacac
    10681 gatggtgcga attttgcggc acgcggggaa ccgggtgcaa attcagaact atatcgacaa
    10741 tacgggcgtg caagtggcgg acgtggtgat cggcttcctg cacatggagc agcggacacc
    10801 cgtcggcgtg aagatgatgg cgatggagcc gaagttcgat tatcactgtt gggatcttta
    10861 cgcgaaagcc acgaactttc ttgccgaaga taaggcgcgg gcggcgaagc tgcgcggtga
    10921 aacgctgaag tcgatcgaag aaggcaaagg cgaagacgcg gaagtggcgc aagtcgtggc
    10981 ggacgcgatt gtgggatgcc acctgcgcac catggcgcgg ctggatatcg gctacgagct
    11041 gctggcgcgg gaaagcgaga tcctgcatct gaagttttgg gatgcggcgt ttgagatgtt
    11101 gaagaagtca ggcgcgattc aactggcgac gagcgggaag atggcgggct gttggatcat
    11161 gccgtggaaa gatgaagagc attcaagaat taacgcagag gggacaggga acacagagga
    11221 cgcagagaag acagaggagg acgagaattc gcaggacaag attattgtgc ggtcgaatgg
    11281 aacggtgacg tacgtcggga aggatattgc gtaccagctt tggaaattcg gattgctggg
    11341 caaggatttt cactaccgga agtggccgac accgccgcag gcggcagatg aaaaagacgc
    11401 gccggagggg cagacggttt gggctacgac gagtggggcg aacgatacgg aggcgccgca
    11461 ctttggcgag ccggcatcga gcgtttacaa cgtgattgat gcgcgacagg cgtatttgca
    11521 gaaggtggtg gcggcggggc tgcgtgcgct ggggcatgcg aaagcggcgg aacagtcgat
    11581 tcacttgtca tacgagatcg tggcgctgac gccgcgatgc gcggcggaaa tgggctacga
    11641 gcttacgccg gaagaggcga agaagccgca tgtggaagtg agcgggcgca aaggttttgg
    11701 cgtgaaagca gacgacctgc tggacaagct ggaggcggcg acgctggcgg aagtgaagaa
    11761 gcgtcaccca tcacatcatc cagcggttcc agacctgccc tcatatgcga ctgtagaaca
    11821 gcatcagatt gcccatgcaa ttgcggttgg tgcgctccga ttcttcctgc tgaaattcac
    11881 acgtaatgcg attatcgcgt tcgactttaa agatgcccta agctttgagg gcgagacggg
    11941 gccgtattgc cagtatgcgg tggtgcgaat tcgcgggatt cggcggaagg gcgcggaagc
    12001 gggggcgacc aacgtcgagg taacgaagga atcggtcagc caactgcttg ccggagcgga
    12061 ggaaaattcg ctttgggagt tactggtggc ggcgggctcg ctggattacg ctgtggatgc
    12121 cgcgattgcg acgcaggagc ccgcgttcat ggcgaagtat gcgtttcaat tggcgcaggc
    12181 gtttaataat ttttatcaca agcaccatat cttgtcggaa gcggatgaac agaagcaggc
    12241 gtttctcctg cggctgacgg aattggtgga ggcgcagttg gtgcgcgcac tatcccttct
    12301 cggaattgaa tcgccggaaa aaatgtagtc tggccggagc aaccccatga agacattgcc
    12361 gccggatctt gaagaggtta tcgggaaagc gctgcagaag agtcagagcg ttccgggatt
    12421 tcttggcgac aacgaggcgc gctttctggc attgctggcc gcgtgcactc cggcatcggg
    12481 cgtcatcgtg gagatcggga gcttcaaggg caagtccact gtgttgctgg cctcggtggc
    12541 cgagcgctat ggactggggc ccgttgtggc cattgatccg cacacggctc cgtcggtcac
    12601 cgatccgaag attgcgccgg gctcatcaac ctacgaggag ttcgtagggg cattgagatc
    12661 gacgcaattg gaagaacagg ttgaaatccg ccgcgtctat tcccgtgagg cggcgaaggg
    12721 ctggaaccgc cccatccggc tactctggat tgacggggat catacctatg agggcgcgag
    12781 agaagatttc gaattgttct cgccttatct cgcgaagggc gcaatcgtgg caatgcacga
    12841 cgcgctgcat gcctatgaag gaccgattcg ggtgttcgtg gagaggattc tccggtcgga
    12901 ccagtttgga ccaacgggat tcgtccagtc cgttgcatgg ggtcaattcc gccctgaggg
    12961 tggagccacg tttcgaatgc agcgagaaaa gttggaaagc cgggcggtgc ggctcctgcc
    13021 ctatttggat aagaagcgcc cgtttcgagg atttacaaag ctgggctaca aactagcgag
    13081 gtcgcgcgtg ccccgcgacg ccatttcgcc ggtctcgtgg tccgatctag tctcggggtg
    13141 aatagcggac ttcacgcgca aaaaagatca gtaagaaagg acgaggtgga cgagggtgcg
    13201 gagggcgacg ccggtggggc ctttggcgag ccagggcttg gcgtcgtcgt tccaggcggt
    13261 gccggcgatg tccaggtgaa tccacggcgt gtcgccggtg aattccttga tgaacatggc
    13321 gccggtgatg gcgccaccgc ctttgccgct gctgatgttc tggatatcgg cgaaagatcc
    13381 cttgataaaa tcgcggtagt cgtcgtcgat gggcatctgc cacatttttt cgccgacggc
    13441 tttcgagctt gccaggagct tgtcggtaaa gggctggtcg gagccgaaga cgccaacatt
    13501 gacgttggcg agcgcgacga cgatggcgcc ggtgagagtg gcggcgtcga cgagatgcgt
    13561 ggcgccgagt tgcttcgcgt agtggatgcc gtcggcgagg atgaggcggc cttccgcgtc
    13621 ggtgttcagg acctcgatgg tctttccgga catggcggtc tggatgtcgc cgggcttttg
    13681 cgctttgccg ccgggcatgt tttccgtgga aggcacgacg cagatcactt tgacgctggg
    13741 cttgagcgcg gcgagagcgc gcatgacgcc gagcatggtg gcgccgccgg ccatgtcgta
    13801 cttcattttt tccatgccgt cggcgggctt gatggagatg ccgccggtgt cgaaggtgac
    13861 ggctttgccg acgagaccga tcaccggagc gccgggcttc aaattcgcgg gcgtataagt
    13921 gacgaccatg acgcgcggcg gctcaggacc tccctgggcg acactgagga gcgcgcccat
    13981 cttgagatca gcaattttct tttcatctag aatctcgacg gtgagaccgg cttccttggc
    14041 catggcttcg gccttttcgg ccaggatgcg cggcgtgagt ttgttggaag gttcgttgac
    14101 gaggtcgcgc gcaaagtttt gtgcgtcggc gatgatccgg cccttcgaaa gacctttgtc
    14161 tccggcggcg cgttcggcgt cggagaagcc agcaatctgg actgattcga tattcttgtc
    14221 gtttttcttg tcggtcttgt atttgtctgt ttcgaaattg gcggtgagtg cgccctcggc
    14281 gatggcttgg gcgatttctc cggtggcgtt gccctctcga acgaggagag cgaaattctt
    14341 gacggagcga gccttcagat accgaagagc ggcaccggcg atcttgcgaa gtgctgcggc
    14401 attgaattgc tcgcgcttcc ctgctccaac gaggagcaag cgtgcggcct tgaggccagc
    14461 gggcgcgtgg atcaaagtga actccagcaa cttgccggtc aattcgccgc tcttggcgag
    14521 cttgcgcagc aggcctgcgg cggcctgatc gatctccgcg atgcggccct gagcggggtc
    14581 agcctcttcg aatacgtacg aaaccagggc atcggtttcg agtgttgcaa acggcttggt
    14641 ttccaacatg atctgcatgg tgctctatcg cctccgattg ttgtacatgg attgttcgac
    14701 ttccctgcgc gcttccttgg tgcgttcatc ctgtcggcgg tcccaggact tcttgccttg
    14761 cgccagggcg atttcgacct tggcgatgcc gttacgaaag tagatcttca atgggatgac
    14821 ggtcaaaccc ttggactcgg tgcggccgga gagcttgtcg atttccctct tgtgaagcag
    14881 gagctttcgg gagcccaaag gctcgtggtt ccagggaccg ccaggtaagt actgtgcaat
    14941 gtgcgcgtta acgagccaag cgctgttgga cttgaaatcg acataggcat cgcgaatgtt
    15001 ggccttgcct tcgcgaaggg ccttgacctc ggtaccgtga aggaccaagc cggcctcatg
    15061 tttctcaaga ataaggtagt tataggaggc cgcgcggttc tgcgcgacga tttgttcgcc
    15121 ggacttcgcg gacttctctg ttttttttgc aggcttggcc acaaccttcc atcatagctg
    15181 ctgtattaaa gcttgtcacc ctcgagacag gatggtgtta tttcatgcac gacagccaac
    15241 ctaagcgtcc ttgccgggct tctaaaacgg gtgatagact gcttgggctt cgtgtcgagg
    15301 cctcggagag tgcatgcgcc gctggggaag cctatttctg tgtgttggtt tcggactgct
    15361 cgccgctggc tgtccgaaag gccaacctga tttcaaccaa ggtaagaaag cggagacgat
    15421 tcaggattac gacgccgcgt tcgtcttcta ccaaaaggcc gctaaggccg acccgtataa
    15481 ctccagctac aagatcaagc tgaaccagat acgcttcgaa gcgagccaac aacacgtcaa
    15541 ggcgggagtg gaactgcgga aaaaaggcga cttgcagggc gcagcaagcg aattccagcg
    15601 ggcgcaggcg atcgatcctg cgagcccggt ggcggaccag gaattgcaca agaccgttgg
    15661 catgatcgcg gagaagaacc gcgcggcgga tgctgccgcg gagcccccgc cggatccaaa
    15721 cgaccagcct ttggcggaga tgccgccgga aatcaagccg ctctcgcgcg cgccgatcaa
    15781 ttataaagcg tcgaatgacg cgaagattgt tttcgacacg atcggaaagc tggcaggctt
    15841 gaccgtcatt tacgacccgg acttccctgc ccggcgcatc tcggtggatt tgaacaatgt
    15901 gacgctggag caggcactgg aaatcgtgtc actggagagc aaggcttttg tgaagccagt
    15961 gacggaaaat atcattttcg tgattccgga tcagccgcaa aagcgccggg actacgaaga
    16021 gcaagtggtc aggacatttt acctttcaaa cacagtacag ccgcaggatt tgaccgagat
    16081 tgtgaccgga ctgcggcaat tgctggattt gaagcgcatc cagcagctca actcgcagaa
    16141 tgccatcatt gtccgcgaca caccggacaa gctgctgctg gcggagaaga tcattcggga
    16201 catcgacaag gccaagcccg aggttgtgat acaggttgaa gtgctggagg cgcgcaccga
    16261 ccgtttgcgg gacttgggca ttcttcccgg gcaaactgct tccattgcga tcaacccgaa
    16321 cggcaccgcg agcaccccta cgggcggcac tcccgcgaca cctaacagcg ggatcacgct
    16381 cagccaactg cgccacctga atggaggcgc ttacgccgtt accctgccga gcctgacggc
    16441 aaacgccgtc ctgactgata cgaacacgag gattatccag aacccagaaa ttcgctcgtt
    16501 ggatggccaa accgccaaac tgcgcatcgg cgatcgcata cctattgcca cgggcagttt
    16561 ttcgtccggc atcggcggag tcgccggggg agtcgcgggc ggcataagtc ccctcgtgaa
    16621 tacgcaattc acctatctgg atgttggcgt gaatatcgat ctgacgcctc gcgtgcaccc
    16681 gaatcgcgac gtcagcctga agttgaaagt ggaagtctcc tcgcatactg gcgaccagtc
    16741 aattggcggc attacgcagc cgatcatcag ccagcgagtc atcgaagatg acatccgttt
    16801 gaaggaaggc gaggtcaata ttgtgggcgg cttggtccag cggaccgaga gcaaaaccat
    16861 agaaggctgg ccaggtctcg cgaagctccc cctgctgaga tatctcttct ctcacaacaa
    16921 gacggaccat caggaagacg aagtgctgat cgtcctgacg ccgcgcatcg tgcggattcc
    16981 ggaatggacg aaagcgaatc tgcggccact atattcgggc tccgagacaa acgtgcaagt
    17041 gaagcgggag agcgagattc gcgcaccggt gcagcagcct cctcctgcgc aagcgcagcc
    17101 gccggcctcg aatcaaaaca tgggagcggc aactcccgcg cccgctcccg gcgccgcggc
    17161 acccgcggct ggggcaattc agggagctcc tgctgccaag cttcgctttg aaccgcagag
    17221 cttgtcgttg aaggccgggc agacggccac aatcggcgtt gtggttgaca acgtgagtga
    17281 tctcttctcg atcccgatgt tgttgcagta caaccccgcg gtaatcagcg tggaagaagt
    17341 gcagcacggc ggattcctct ccgggggaac gcaggaaatc gccatcgtgt atcagccctt
    17401 caaggataaa gggcaggcga ttatttccgc cacgcgccgg ccgaacacgc caggagtgag
    17461 cggaagcgga acgctgatcg gaatcgtcgt caaggcgctg gcgcccggct catcaaacct
    17521 gtcgatcgtg caggtgaatg cgaaagattc gcagcagaag ctgataccgc tgatcaccag
    17581 cgaagcgacg ctgcaagtcc agccgtagtc gaattgtgcg agcgatgttg cgcgacactt
    17641 caaaccaccg gcgaatgggc agacgaatcc aacggagacc gttttcctcc gagacgggga
    17701 tgacgctgct ggagttgatc atcgcctgcg ccattctgct gattctgtct tccgccgcgc
    17761 tgcccatcgc gaaatacacc atcattcggc agaaagaagc ggagctccgc cgcgatctcc
    17821 gggagatgaa ggatgccatc gaccgctaca aagacgcggc cgaccggaac cagatccgcg
    17881 tggagatcgg aagcgaaggc tatccgccgg acctggagac gctggtgaag ggtgtgcagc
    17941 tcggcgcgag cagcgataaa aagattcgtt tcctgcggaa gatccccgtt gatcccatga
    18001 ccgggcgggc cgaatggggc ctgcgcgccg ttcaggacga tcccgactcg acgagctggg
    18061 gcggcaagaa cgtgttcgac gtatactcga aatcatcggg aacggcgctg gacggcacta
    18121 gatattcgac ctggtaattc aagcgaggat tcgatttgaa gaggctgctg caaactcgga
    18181 acggacggca ttcactgcgg aaacgccctg gccgtctgtt cgaaaccggc ttcacgctga
    18241 tcgagttgat gatcgtcatt acgatcatct tcattctggt gggcatagcg gcggtgcgct
    18301 tcgataagtc cgtgcagcgc gcaaaggaag cgaccttaaa acaagatctg caggccatgc
    18361 gccagtccat cgacaactac acgctcgaca aacaggctgc gccgcaatcc ctggaggacc
    18421 tggtgcaggt gggttacctg cacagagttc cgacagaccc gatgacggga gcggccgact
    18481 gggtgccaca attcgacagc gtggtgctga gccctgagca atcgagcacc gggatggttg
    18541 acgttcattc gaattccgcc agagtctcat ccttcgaaaa cacaccctac aacgagtggt
    18601 agccattctc ttccacgttc gaaatattgt tccgccagtt ttgaccgggc tacgcgctga
    18661 tttgacggaa cctcgcatgt ccgctatagt ggctgtgcgg ggtggagcag cctggtagct
    18721 cgtcgggctc ataacgcgcc aattatcacc cggctctgcc ggattcaaac gacttaaccc
    18781 ttcttcccag ccgcaaaaac ctctcaaaac gggatattct gggcacttaa cgggcacctg
    18841 aatgaagagg cctttcacat cgggcgagtc gtggaagaag gatgttgggt tcgatcccca
    18901 ccgtccctat caaccttttt cccaacaata acttagcgat ttgtcgaggc ggccaaaggc
    18961 ggcaagaaga gcctcacggg tggcacccat ggctctggat aggcgcgctg gacggtcaag
    19021 taaattgttt gcg
//
LOCUS       DASYVO010000487        11800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_192063, whole genome shotgun sequence.
ACCESSION   DASYVO010000487 DASYVO010000000
VERSION     DASYVO010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 11800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11800
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192063"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1903
                     /locus_tag="VGR03_16865"
     CDS             <1..1903
                     /locus_tag="VGR03_16865"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEV1996006.1"
                     /translation="VCLMDCLAQGAAQLGFDDVDAFLESVELIRWSTTITSNVLAEQR
                     GPKLGLIVSRGHETAFYGDEQCRRLLDKLISPGSVIALDPSAPETEVMNVVRSLLESG
                     VRRICISLAGSDLNPEQEIRMKRIVERQYPDHFLGSVPVLAGSDIAPGADDRTRTYCA
                     LLNAFTHGALAATLFRAEDELRETHHYHGNFFVSHINGGVAGIAKTKAIDTMESGPIL
                     GIHGSSHLARAFGLNEVIALDVGGTTAKLSVLRNAEPIFRKPSDLFGIPVGMSLPYLR
                     SMALGGGSVVKPAGSPARPQLSLGPESMGAFPGPACYGLGGDQPTLTDAFVCAGLINP
                     EYFLGGAKPIDPALAHRAIQEFVAGPLHFSVEEACRAAIDRAFQSVGEMIANAQSDLR
                     QNFSNHALFAYGGNGGLFACGIAEKAGLDRVYFFSLGPVFSAFGSSVSDICHLYERPV
                     HLTALEEKDVSRLRQDLDDMKAEGLKDLLGEGIRPADVTYRFEMEVSQTGQPSKSVCT
                     PESALRSAKDLRAHLTGGLHDEQASKDGDFFLDLARLQVKKAMPKPSFLERPLQGADS
                     SHARKGIRPVAWGSKSGSAQLYRWEALQPGNRVEGCAVLEGVHSTYFVPEGWLLEMDP
                     YGNGKLRRQ"
     gene            1960..2346
                     /locus_tag="VGR03_16870"
     CDS             1960..2346
                     /locus_tag="VGR03_16870"
                     /inference="COORDINATES: protein motif:HMM:NF020456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetone carboxylase subunit gamma"
                     /protein_id="HEV1996007.1"
                     /translation="MSSEQQNRIRITEYLDLDLDREQWMCNRCGHVLVSARENYKQGC
                     LLHDRDPREIHQPILSGEFNFSPDPLWVRIVEFYCPGCGTQIETEYLPPGHPITRDIE
                     LDLDRLKERIRQGEFVIRGQRLEVAE"
     gene            2343..4463
                     /locus_tag="VGR03_16875"
     CDS             2343..4463
                     /locus_tag="VGR03_16875"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEV1996008.1"
                     /translation="MNSIDIDVGGTFTDLVLNFRGKTLIKKVPTTPYDLSVCFSRVIE
                     EGASAIGVKVEDLLPAVEMIRYSTTIALNRLIEKKGPRLGLITTEGHEDVILIGRGAQ
                     WIDGTRVAERRNLAVQKKPDPLIPRDLIVGVKERVDSRGRVLRPLDENDVREKVRYLV
                     NRGARGLVVSLLWGFLNPLHERRIKEIIRDEYKEFHIGYLPVVLAGQVVGKLGEYERT
                     LAAILDAYLQRSMQIELSAMWDKLRDRGYKKPLLMIQSSGGIAEVFRTTASRTFNSGP
                     VSGLMGAHHVAKLLGHKNVVMTDMGGTSFDVGLVVEDSVRSYDFRPIIDRWMVGITMI
                     KTLSIGAGGGSIASIIKLLRNQVQVGPRSAGSLPGPACFNLGGSDATVTDADLVLGYL
                     NPEFYFGGKMRLDKNRSVQAIRDKIARPLGIAVEEAAAVIRKIVNGNMASAIMKEVHL
                     RGYSPEDFILFVGGGAGPTHVEGFMAGIERAVTFAFSPVFCAFGSSTMDIMHVYEVSR
                     KLTLMEPVTMQLTNDYEIFNQTVEALMVQARRDLRADGLDLDQASFVLELDMLYGGQF
                     HVKRALSPKLSLHRPEDVRAICDAFKKEFSEAFSPFVVNPEGGVFVESFILKAIVPVR
                     KIDLPKLALEGRDPEAARKGQRAVYWPQAGDFQPTPIYAYESLRPGNLVEGPAVLEGE
                     YTTLVVPPSMRFSIDQRGLGILES"
     gene            4489..6549
                     /locus_tag="VGR03_16880"
     CDS             4489..6549
                     /locus_tag="VGR03_16880"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEV1996009.1"
                     /translation="MPIPTLSEKLQWLKPVPPTPEETRCAGKIRQGDYEIGVQTTNDI
                     LDEAMEIFIRSSRSYFGVSGDSMVAIFSANGDLINASCGTYLHAIIQPIVIKFILKYY
                     EKNPGIRDGDIWFTNDALYGGIHNPDQVVLMPVFHQGTLLAWTSAALHTTETGAIEPG
                     GMPVSAKSRFEEGLNLPPIKIGENFELRKDFLEFYMVYGLRAPAMFISDLRARCTTAD
                     RVRVRLLELVAKRGQEHLVGLLRKMLETAEAGSRQKIKALPEGKFRTVVFNDAIGWTP
                     ALVRACYLTLTARDGRLIFDFTGTSPENPSSYNVHSQAVVGHIANFMYEYVFHDLPIC
                     SATFASIDFVFPEGIILNPDPMAATSCCVFIGMQARCATHNSFAKMTFSSAELWRQIA
                     AAPGSQHTSQICAGHSQWNLAFADVLSFSLNTQGQGGRASADGMDSYGFAWCAFGRAP
                     DSEQVESELPVTVTLSQHWKDSSGPGCHRGGSGGVQQWMIHKVPSMVSMCMGNGSKVP
                     LGQPLFGGYASSPIPGISIRKADLLQRMEKGDPELSMDHRAILEKGTIQGEWSCELVA
                     RTPKVFEEGDLLFGFSGGGPGYGDPLEREPAEVLEDLRKNIISDWTAQNIYRVAYDPE
                     RRKLDAEKTRQQRDAERRARLARGKSFDEFHQEWNKMSPPKEILQWYGSWPDAKPTGP
                     IFRP"
     gene            6553..7578
                     /locus_tag="VGR03_16885"
     CDS             6553..7578
                     /locus_tag="VGR03_16885"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEV1996010.1"
                     /translation="MAAQPTPREIVKGLLQGIAAPRPLFLPIVFSAGSRVADVSLRAF
                     MDNPTKISNSLRQIRGHLPSDGLACYFDLYLEAESLGGTLQWGTEEQPPTLHWPPLTK
                     PGELPPGLRTPEEAAKSSRVAVAVEVIRRLRTLRRDDSLLMAGLTGPYTLAARITQLE
                     LKDPLRSEDLPERALEIAAAVISQIAAALVEAGANLVFIQEKVLPALSAEGCEAWASL
                     LAPAFNIIRFYEALPVLQLTDTRSFAQNSEVIFQREWDCVVCPALPEFPSPPSERFSA
                     LKAVALGISLPLEVFQPDRSEGDRLQQYVHQIISELRPAILTTAGDVPPMTDMKRIIE
                     VCDYVRR"
     gene            7629..7817
                     /locus_tag="VGR03_16890"
     CDS             7629..7817
                     /locus_tag="VGR03_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996011.1"
                     /translation="MATRHESVESASEQQLHECGPALRKGRRELARELRRSNLRYGIA
                     SACIGGGQGIALLLENPR"
     gene            7827..8312
                     /locus_tag="VGR03_16895"
     CDS             7827..8312
                     /locus_tag="VGR03_16895"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEV1996012.1"
                     /translation="MLINRKKIYIEFGDCDPGGIVYFPRYFEYFDACTNALFERAGLP
                     KPQMLKTFGIAGIPLVEAQARFLAPSQFGDTVMVESRVAEWGRSSFSVHHKLFKGKVL
                     AVEVFEKRVWVKRAADGPMPFKGQTIPEEVKERFSRRAGRHDLHHPGKKRESDDDHTD
                     A"
     gene            8710..9936
                     /locus_tag="VGR03_16900"
     CDS             8710..9936
                     /locus_tag="VGR03_16900"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEV1996013.1"
                     /translation="MLRLRSKTKKVATAALAFLASFSLALPTALPINAADHAESTSVA
                     GDPGADIADVFAFLDPSDNSKVVLAMDVEGFVVPSELLNLSFFAPDVRYRFEIENTGD
                     AIPDQRIDVTFSPQISRSQPQMATIRLPHGQMFTAPTTVQTLAPAANPFVVTTDPASG
                     VSFFAGLTDDPFFFDIVGFNRFVSSVLAGAPDPTKLQRNRDSFAGYNIHMIALSVPAW
                     MLRGRADNNVIGVNGVTLRRKNSHREESEDGEREGKLVQVDRMATPAVNTALIPFPRK
                     NEYNAATPVDDAAGRFAGSIVGTLTALGTNATNIGILANVAVVRGDYLRLDLSKPNAS
                     VGFGENITTPNYTGFPNGRRPGDDTINTLLYFITNQSGIQEAVNHNDVPLGSTFPFFA
                     PPNQPLENPAIDNTMN"
     gene            10061..11452
                     /locus_tag="VGR03_16905"
     CDS             10061..11452
                     /locus_tag="VGR03_16905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1996014.1"
                     /translation="MRGKRIIVVSMAALGVAVLTLNAYRNPRATSVGLTRLPLPPAVL
                     LPSDRAATEQTIRFLENRIQRDPEDFIAFNKLASYYLQRVRETGDLTYLKLASHASRA
                     SLATLPAERNIGGLTVLAQSEFTSHEFAASRDHARRLTELEPDKSYPQQILGDALLEL
                     GEYQKAKEAFWQMEQLGGIQGLTRVATEQRIARLAALHGDTVAALSHMQNATALAQAM
                     PVPPRETVAWCRWQLGELAFSRGDYAAAEQHDRDALTTFPDYYRALASLGRVRAARGD
                     LSAGIEQYERAISLLPDPSFVAALGDLYELTGRDKDAAAQYALVEAIARLSVASGTLY
                     NRQQALFHADHNIKPQAAYANAAKEYAVRKDIYGEDAVAWTALKARKLPEAQAAMKEA
                     LRLGTQDAKLFYHAGMIAQAAGDKASARDYLKRALTLSPQFDPLQSSIARKTLASLVE
                     EDQRKADVTNRGE"
     gene            11469..>11800
                     /locus_tag="VGR03_16910"
     CDS             11469..>11800
                     /locus_tag="VGR03_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996015.1"
                     /translation="MVPNNKTIPRLQRVAVLAACWLLSCPASFAHPMGNFSISHYAGI
                     HIEQGFIELRYLIDLAEIPAFQEMQQNDIVARVDDPRVLAYLSRQAEVFKKGLLLTLN
                     GQPLPLQIT"
BASE COUNT         2441 a         3632 c         3375 g         2352 t
ORIGIN      
        1 cgtctgtctc atggattgtc tcgcccaggg agcggcgcaa ctcggcttcg acgacgtgga
       61 tgcgttcctg gagagtgtcg aactcatccg ctggtccacc acgattactt ccaacgtact
      121 ggccgagcaa cgcggcccca agctcgggct gatcgtttcg cgcggccacg agacagcctt
      181 ttacggcgat gagcaatgcc gccgtctcct ggacaaactc atctccccag gtagtgtcat
      241 cgctctggac ccgtccgcgc ccgagaccga ggtcatgaac gtggtgcgct ctctcctcga
      301 gagcggcgtc cgtcgcatct gcatcagcct ggcagggtcg gacctcaatc ccgagcagga
      361 gatccgcatg aagcggatcg tcgagcgcca gtatcccgat cattttctcg gctctgtgcc
      421 cgtcctcgcc ggcagcgaca tcgcgcccgg cgccgatgac agaacgcgga cgtattgcgc
      481 gctgctcaac gctttcaccc acggcgcgct ggccgccacg ctgttccgag cggaggacga
      541 gctgcgcgag acccatcact accacgggaa tttcttcgtg agccacatca atggaggcgt
      601 tgccggcatc gccaagacaa aagccatcga caccatggaa tcaggtccga tcctcggcat
      661 ccacggcagc agtcatctcg cccgggcgtt cggactcaat gaggtcatcg cgctcgatgt
      721 cggcgggacc accgcaaagc tgagcgtcct ccggaatgcc gaaccgattt ttcgcaagcc
      781 gtccgacctc ttcggtattc ccgtgggcat gtctcttcct tatctgcgct ccatggccct
      841 cggcggaggg agtgttgtga agcccgcggg atctcccgcc aggccgcaac tttcgcttgg
      901 gcccgagagc atgggcgcct ttccggggcc ggcgtgctac ggactgggag gcgaccaacc
      961 cacactcacg gacgccttcg tctgcgccgg actcatcaac ccggagtatt ttctgggagg
     1021 agccaaaccc atcgatcctg cgctcgccca ccgcgccatt caggaattcg tggccggtcc
     1081 cctgcatttc tcggtcgaag aggcctgccg cgccgcgatc gaccgggctt ttcagtcggt
     1141 tggggaaatg attgcaaatg cacaatctga tctccggcag aatttctcca accacgcgct
     1201 ttttgcctat ggtggaaacg gagggttgtt tgcgtgcggc atcgccgaaa aggccggcct
     1261 cgatcgagtc tatttctttt cgctcggacc cgtgttcagc gctttcgggt catccgtctc
     1321 ggacatctgt catctctacg agcggccggt ccatctgacc gcgcttgagg aaaaggacgt
     1381 cagccggctg cgtcaggatc tcgatgacat gaaagcggag ggtctcaaag atctgctcgg
     1441 cgagggcatt cgcccggccg atgtgaccta tcggttcgag atggaagttt cgcaaacggg
     1501 gcagccctcg aaatcggttt gtacgcctga atccgcgctc cgcagcgcca aggatctccg
     1561 cgcacatctc accggaggcc tccacgatga gcaggcctcg aaagacggcg acttcttcct
     1621 tgatctcgca cgcctccagg tcaagaaggc gatgcccaaa cccagcttct tggagcgccc
     1681 tctgcagggt gctgactctt cccacgctcg aaagggaatt cgcccggtcg cctgggggag
     1741 caagagcggc tcagcgcaac tgtatcgctg ggaagcttta cagcccggaa atcgcgtgga
     1801 aggttgtgcg gtgctcgagg gcgttcatag cacctacttc gttccggagg gctggcttct
     1861 cgagatggat ccgtacggga acggaaaact tcgccggcag taggcaacac cggaaccagt
     1921 atttgcggca cccaggattt gcaggacaag gacacggcca tgtcatccga acagcagaac
     1981 cgcattcgaa tcaccgagta cctcgatctc gatctcgatc gtgaacaatg gatgtgcaac
     2041 cgttgcgggc atgtcctggt ttccgcgcgc gagaactaca agcagggctg cctgctccat
     2101 gaccgcgacc cgcgcgagat tcaccagccc atcctctcgg gggaattcaa tttctcaccc
     2161 gatccgctat gggtccgcat cgtcgaattc tattgtccgg gctgcggcac tcagatcgag
     2221 accgaatatc ttcctccggg acatcccatc acccgggaca ttgagctcga cctcgatcgg
     2281 ctcaaagaga gaatccgcca gggcgaattt gtcatccgcg gtcaacggct cgaggtcgcc
     2341 gaatgaactc cattgacatc gatgtgggcg gaaccttcac cgacctcgtc ctcaatttcc
     2401 gcggaaaaac cctgatcaag aaagttccca ccacccccta cgacctgtcc gtctgcttct
     2461 cgcgcgtgat cgaagaaggg gccagcgcga tcggcgtcaa ggtcgaagac ctgctgccgg
     2521 cggtcgaaat gatccgttat tcaaccacca tcgcgctcaa ccgcctcatc gaaaagaagg
     2581 gcccgcgcct cgggctcatc accaccgaag gacatgagga tgtcatcctg atcggccgtg
     2641 gcgcccaatg gattgacggc acccgcgtcg ccgagcgccg caacctcgcg gtccagaaaa
     2701 aaccggatcc cctgattccc cgcgacttga ttgtcggagt caaagagcga gtcgactccc
     2761 gcggaagggt cctgcggcct ctcgacgaaa atgacgtgcg ggagaaggtc cgttacctgg
     2821 tgaatcgcgg cgcgcgggga ctggtcgttt ctcttctctg gggatttctg aacccgcttc
     2881 atgagcggcg cataaaggaa atcattcgcg acgaatacaa ggaatttcac attggttacc
     2941 tgcccgtcgt cctggccgga caggtggtcg gcaaactggg ggaatacgag cgcaccctcg
     3001 ccgccattct ggacgcctac ctccagcgct ccatgcaaat cgagctctcg gccatgtggg
     3061 acaagctccg cgaccgcggc tacaagaagc cgctgctgat gattcagagt tccggcggca
     3121 ttgccgaggt tttccgcacc accgccagcc gcaccttcaa cagcggaccg gtctccggac
     3181 tcatgggcgc ccaccacgtc gccaaattgt tgggccacaa aaatgtggtg atgacggaca
     3241 tgggtggcac gagcttcgac gtcggcttgg tggtcgaaga cagcgtgcga agctacgact
     3301 tccgcccgat cattgaccgc tggatggtcg gcatcaccat gattaagacg ctctccatcg
     3361 gcgccggcgg cggctccatc gcctcgatca tcaagcttct tcgcaatcag gtgcaggtcg
     3421 gtccgcgaag cgccggctcc ctgcccggtc ccgcctgctt caatctcggc ggcagcgatg
     3481 caaccgtcac cgatgcggat ttggttcttg gttacctgaa tccggaattc tatttcggcg
     3541 gaaagatgcg cctggacaag aatcgctcgg tgcaggccat ccgcgacaag atcgcccggc
     3601 ccctgggcat tgcggtggaa gaagccgccg cggtcatccg caagatcgta aacggcaaca
     3661 tggcctcggc gatcatgaag gaagtccacc tgcgcggcta cagtccggag gatttcattc
     3721 tcttcgtcgg aggcggcgcg gggcccaccc acgtggaagg gttcatggcc ggcatcgaga
     3781 gggctgtgac tttcgctttc tcgcccgtgt tttgtgcctt cggttcgtcc accatggaca
     3841 tcatgcacgt ctatgaagtc tccaggaaac tcacgctgat ggaacccgtc accatgcagc
     3901 tcaccaatga ctatgaaatc tttaatcaga ccgtcgaagc cctgatggtg caagccagga
     3961 gagatctgcg cgccgatggg cttgatctgg atcaggcttc cttcgttctc gagctggaca
     4021 tgctctatgg cggacaattt cacgtcaagc gtgctctgtc gccaaagctt tccctgcacc
     4081 gcccggaaga tgtccgcgcc atctgcgacg ccttcaaaaa ggaattcagc gaagcgttca
     4141 gtccgtttgt cgtcaatccg gaaggcgggg tatttgtcga gagtttcatt ctgaaggcca
     4201 tcgtgcccgt gcgcaaaatc gatctgccca agctggcgct ggaggggcgc gatccagagg
     4261 cggcacgaaa gggccagagg gctgtctact ggccgcaggc aggagacttc cagccgacgc
     4321 cgatctacgc ctacgaatct ttgcgtcccg gcaatctcgt ggaagggccg gccgtcctcg
     4381 agggcgaata caccacgctg gtcgtacccc cttcgatgcg gttttcgatt gaccagcgcg
     4441 gtctcggcat tctggagagc taggccaccg gagaaatcga ggagacatat gcctattcca
     4501 acgctgtccg aaaaactcca atggctcaaa cccgtcccgc ccacgcccga ggagacgcgc
     4561 tgcgccggga aaatccgtca gggcgactat gaaatcggcg ttcagaccac caatgacatt
     4621 ctggatgaag cgatggagat ttttatccgg tcctcgcgga gctacttcgg cgtttccggc
     4681 gactcgatgg tggccatctt ctcggccaac ggagatctga tcaacgcatc ctgcggtacg
     4741 tatttgcacg ccatcatcca gccgatcgtc atcaagttta ttctcaagta ctacgaaaag
     4801 aatcccggaa tccgcgatgg cgacatctgg ttcaccaatg acgccctcta cgggggcatc
     4861 cacaatcccg atcaggtcgt gctcatgccg gttttccacc aggggactct gctcgcctgg
     4921 acctccgccg ctctgcacac cacggaaacg ggcgccatcg agccgggcgg catgccggtc
     4981 agcgccaaat cgcgatttga ggaggggttg aatctgccgc cgattaaaat cggagaaaac
     5041 ttcgagctcc gcaaggattt tcttgagttc tacatggttt acggtcttcg tgcgccggcc
     5101 atgtttattt ctgaccttcg cgcgcgctgc accaccgcag accgcgtccg cgtgcgcttg
     5161 ctggagctgg ttgccaagcg cggccaggag cacctggtcg ggttgctgcg caagatgctg
     5221 gaaactgcgg aggccggctc ccggcagaag atcaaggcgc ttcccgaagg aaagttccgc
     5281 accgtcgtct tcaatgacgc catcggatgg acgccggcgc tggtgagggc ctgctatctc
     5341 actcttaccg cccgggacgg aaggctcatc ttcgatttca ccggcacttc tccggaaaac
     5401 ccatcgtcgt acaacgtaca ctcgcaggct gtcgtggggc acattgcgaa tttcatgtat
     5461 gaatatgttt ttcacgacct gcccatctgc agcgccacct ttgcatccat tgactttgtt
     5521 ttcccggagg gaatcatcct caatcccgat cccatggcgg ccaccagttg ctgtgtgttc
     5581 atcggaatgc aggctcgctg tgccacgcac aacagcttcg ccaagatgac gttttcgagc
     5641 gccgagctgt ggcggcagat tgccgcagcg ccgggaagcc agcatacctc gcagatctgc
     5701 gccggccatt cgcagtggaa tctggcgttc gccgacgtcc tctccttctc gctcaatacc
     5761 caggggcaag gcgggcgcgc cagcgcagac gggatggact cctacggatt tgcgtggtgc
     5821 gcgtttggcc gggccccgga ctccgagcag gtcgagagcg agctgccggt gaccgtcacg
     5881 ctctcccagc attggaagga ttcgtccggc cccgggtgtc atcgcggcgg ctcggggggc
     5941 gtgcagcaat ggatgattca caaggtgccc agcatggtct ccatgtgcat gggcaacggg
     6001 agtaaggtgc ccctcggcca gccgttgttc ggtggctatg cctcctcacc cattcccggc
     6061 atcagcattc gaaaggccga cttgctgcaa aggatggaaa aaggagatcc cgagctgtcc
     6121 atggatcacc gcgccatcct cgagaaaggc accattcagg gtgaatggag ctgtgaattg
     6181 gtcgcgcgga ctcccaaggt ctttgaagag ggagacctgc tgttcggatt cagcggcggg
     6241 gggccgggct acggtgaccc gctcgaacgc gagcctgcgg aagttctcga agacctgcgt
     6301 aaaaacatca tctcggattg gaccgctcaa aacatctacc gcgtcgctta cgaccccgag
     6361 cggcgaaagc tggatgcgga gaaaaccagg caacaaaggg acgcggagcg acgggctcgg
     6421 ctcgcccggg gcaagtcgtt cgacgaattc catcaagagt ggaacaagat gagccctccg
     6481 aaagagatct tgcagtggta tggctcctgg ccggacgcca agcccacggg acccatcttc
     6541 cggccctgag agatggccgc tcagcccact ccccgggaga ttgtcaaagg attgttgcag
     6601 gggatcgcag ctccgcggcc tctgttcttg ccgatcgttt tctctgccgg ctccagagtg
     6661 gcagacgttt cgttgcgcgc tttcatggac aaccctacga agatttcgaa ttcgctgcgc
     6721 caaattcgcg gccatttgcc ttccgacgga ctcgcctgct atttcgatct ttatctcgaa
     6781 gcggaatcac tcggcggcac gctgcaatgg ggaactgaag agcagccgcc cacgctgcat
     6841 tggcccccgc tcacgaaacc gggcgaactg ccgccgggcc tgcgcactcc ggaagaagcg
     6901 gcgaagagca gccgcgtcgc cgtcgcggtc gaagtcatcc gccggctccg tacactgcgg
     6961 cgtgacgatt ctctcctcat ggccggcctc accggcccgt acacgcttgc cgcgcgcatc
     7021 acccagttag agctcaagga cccgctgcgc agtgaggact tgccggagcg cgcgctggaa
     7081 atcgccgccg cggtgatctc gcagatcgca gccgctctcg tggaagcggg ggccaatctt
     7141 gttttcatcc aggagaaagt tctgccggct ctttcggcgg aaggttgtga ggcttgggcg
     7201 tccttgctcg cgccggcgtt caacatcatc cggttctatg aagcacttcc tgttctccag
     7261 ctcaccgaca cccgttcctt cgcccagaac agcgaggtga tatttcagcg ggagtgggac
     7321 tgcgtggtat gtcctgccct ccccgagttt ccgtccccgc catcggaaag attctctgcg
     7381 ctaaaggcgg tggcgctagg catctccctc cctctcgaag tattccagcc ggaccggtcc
     7441 gagggtgacc gcctgcaaca atatgttcat caaatcattt cggaattgcg ccccgcgatc
     7501 ctgaccacgg cgggcgatgt cccgcccatg acggatatga agcggattat agaggtctgc
     7561 gactatgtcc gccggtgacc acgcagcccg ccgcgcttct gagtttcggg aaggcggcgg
     7621 taacccggat ggcaacaaga catgaatcgg tcgaatccgc gagcgagcaa caactccacg
     7681 aatgcggacc tgccctccgc aaaggcaggc gggagctcgc gcgcgagctg cgccgctcga
     7741 atctgcgcta cggcatcgcc tcggcatgca tcggcggcgg gcagggcatc gcactgctgc
     7801 tcgaaaatcc tcgttgagaa ggcgaaatgt tgatcaaccg caagaagatt tacatcgagt
     7861 tcggagattg cgacccggga ggcattgtct atttcccccg gtattttgaa tacttcgatg
     7921 cctgcaccaa tgctctgttc gaacgggccg gcctgcccaa gcctcaaatg ttgaagacat
     7981 tcgggattgc gggtattcct ctggtcgagg cccaggcgcg attcctggcg ccctctcaat
     8041 ttggcgacac cgtcatggtc gagtcccgtg tggccgaatg ggggcgcagc agtttctccg
     8101 tccaccacaa gctattcaag ggcaaggtgc tggccgtgga agtcttcgag aagcgcgtct
     8161 gggtcaaacg agccgcagac ggcccgatgc ccttcaaggg acaaaccatc ccggaagagg
     8221 tgaaagagag attctctcgt cgcgcaggca gacacgatct ccatcacccc ggaaagaaac
     8281 gcgaatcgga tgatgaccac acagatgcct agcgcatcgc ttaaatctgt attaaacagc
     8341 ggggccttgg ctgtctaatt tagacgggag catcacatac atctcctttg tttcttcgcc
     8401 caacattagc caaggcaatt tccattcccc gttcgtttca atgcagtacg ctagttgcac
     8461 ccattttgga tgtatttttg cgaaagtttt ctccgaaagc atggtacctg tctgctcccg
     8521 ccaaaaaaga ccggcgcagg acatccaaag acaccgctgc ttccgtagta gcaatgggaa
     8581 ctggaatctt tcgagttccc gccatccagg ttcttgcaag agggcatgaa cgatttggga
     8641 ggggagtatg tctgcttgcg ggaactgtca ccggacggtc catcaaaaat aatcataagg
     8701 agaaaaacca tgttgcgatt aagaagtaag actaagaagg ttgcgactgc ggctctcgcg
     8761 ttcctggcga gtttttcact ggctctgccg acagctctgc ccatcaacgc ggctgaccac
     8821 gcagaatcaa cgtcagttgc cggtgatcca ggagctgata tcgccgacgt ctttgccttt
     8881 ctcgatccca gcgacaactc gaaggtagtc ctcgcgatgg atgtggaggg gttcgtcgtg
     8941 ccctcagagc tcttgaacct cagcttcttt gcgccggatg tgcgctaccg gttcgaaatc
     9001 gagaacactg gcgacgccat ccccgatcaa aggatcgacg tcaccttctc tccacaaata
     9061 tcccgcagcc aaccgcagat ggccacgatc agattgcccc acggccaaat gtttaccgct
     9121 cccaccaccg ttcagactct ggcgccggcg gcgaacccgt ttgtggtcac gaccgaccca
     9181 gcaagcggcg tctctttctt cgccgggttg accgacgatc cgttcttctt cgatatcgtg
     9241 ggattcaacc ggtttgtttc atcggtcctg gccggcgctc cggatcccac gaaactccag
     9301 cgtaaccgcg atagcttcgc gggctacaac attcacatga tcgccctcag cgttcccgcc
     9361 tggatgctca gaggcagagc cgataacaac gtcattggcg tgaatggcgt cacgcttcgc
     9421 cggaaaaact cccatcggga ggaatccgag gatggggaga gggaggggaa acttgtccag
     9481 gtggaccgca tggcgactcc ggctgtcaat acggccctca tcccgtttcc gcggaagaac
     9541 gagtataacg cggccacccc cgtggacgac gcggccggcc gattcgccgg tagcatcgtt
     9601 ggcacactaa cagcccttgg caccaatgca accaacatcg gaatcctcgc caacgttgca
     9661 gtggtcagag gagactactt gcgcctggac ctttcaaagc cgaatgcgag cgtaggtttt
     9721 ggagaaaaca tcaccacgcc aaactacacc ggctttccaa acgggcgtcg cccaggcgac
     9781 gacaccatca acacgctgct ctatttcatc acgaaccaga gcggcatcca ggaagccgtc
     9841 aaccacaacg acgtaccgct gggcagcaca tttccgtttt ttgcgccgcc caaccagcca
     9901 ctggaaaacc cggcgatcga caacaccatg aattagcttg gtgggtcacc gctagcgaag
     9961 ccacatgccc cggcactgat tgcgaatcgt ggtcagcgcc gggggagtgg ctggtaccgg
    10021 tgaccggacg gcaagtttcc acgaggaggg gcgaactaaa atgagaggga agagaatcat
    10081 cgtcgtttcg atggccgcgc ttggagtggc ggtgttgact ctgaatgcgt accgcaatcc
    10141 gcgagccacc tccgtgggcc tgacacgctt gccgctgcca ccagcggtgc tgctgccttc
    10201 cgaccgagca gcaaccgagc aaaccattcg attccttgag aatagaatcc agcgggaccc
    10261 ggaagatttc atcgctttta acaagctggc gagttactac ctccagcgcg tccgcgaaac
    10321 aggcgatcta acctacttga agcttgcgtc tcatgcctcc cgtgcctcgc tcgccacatt
    10381 gcctgcggag cggaacatcg gtggcctaac cgtgctggcg caatcggagt tcacctctca
    10441 cgaattcgcc gcctcacgcg accatgccag gcgattgacc gagctggagc cggacaagag
    10501 ctatccgcaa cagattctcg gtgatgcgct gcttgagctt ggcgaatacc aaaaggcaaa
    10561 ggaagcattc tggcagatgg aacaattggg cggcattcag ggccttaccc gtgttgccac
    10621 cgaacaacgt atagcaaggt tggcagcgct tcatggcgat accgttgccg cgctgagtca
    10681 catgcaaaat gcgaccgcac tcgctcaggc gatgcccgtt cctccgcgcg agaccgtagc
    10741 ctggtgccgc tggcaactcg gtgaactcgc cttttcccgt ggtgattacg ccgcggcgga
    10801 acagcatgat cgagacgcgc taactacctt tccggattac taccgggccc tggcgtcact
    10861 gggccgcgtt cgcgccgcgc gtggcgacct ttcggccgga atcgagcaat acgaaagggc
    10921 cattagtttg cttcccgacc cctcatttgt cgcagcgttg ggagatctat atgaacttac
    10981 gggacgtgac aaggatgcag ccgcgcaata tgcgctcgtg gaggcaatcg cgcgactcag
    11041 cgtggcgtca ggcacccttt ataatcgcca gcaagcgctt ttccatgccg accacaatat
    11101 caagccccag gcggcctacg cgaatgctgc caaggaatat gcggtgcgaa aagacatcta
    11161 tggagaagat gcagtggcct ggacagctct caaagcccga aaacttcccg aggcacaagc
    11221 ggccatgaaa gaggcgcttc gcttgggcac gcaggacgcg aagttgtttt atcacgcggg
    11281 gatgattgcg caagccgcgg gggataaagc ctccgcccgt gattatctca agcgcgcgct
    11341 gacgctcagc ccgcagttcg atccgttgca atcttcgatt gccaggaaga cactcgcgag
    11401 tcttgttgag gaagaccaaa gaaaagcgga cgtaaccaac cggggagagt agcggaagga
    11461 ataaacccat ggtgccgaat aataaaacca ttccccgtct gcaacgtgtc gccgtattgg
    11521 ccgcttgctg gctattgtct tgccctgcaa gcttcgccca tcccatgggt aatttcagca
    11581 tcagccacta cgcgggaatc cacatcgagc agggattcat cgaactgcgc tatctgatcg
    11641 acctggcgga aatcccggca ttccaagaga tgcagcagaa cgacatcgtg gcccgggtgg
    11701 acgacccacg agtgctagct tacctatcga ggcaggctga agtatttaag aagggtttgc
    11761 ttctcactct caacggccag ccgctccccc tgcagatcac
//
LOCUS       DASYVO010000488         7452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_150539, whole genome shotgun sequence.
ACCESSION   DASYVO010000488 DASYVO010000000
VERSION     DASYVO010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7452
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150539"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..387)
                     /locus_tag="VGR03_16915"
     CDS             complement(97..387)
                     /locus_tag="VGR03_16915"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEV1996016.1"
                     /translation="MASSTLRSEKLDLRLTRKAKAALRAAAAASKRSVSEFVLESALA
                     RADEALADRRTFGLNAAQWKAFLAALDAPPRPLLRLEHLFKEPGFFDTGTCP"
     gene            complement(485..793)
                     /locus_tag="VGR03_16920"
     CDS             complement(485..793)
                     /locus_tag="VGR03_16920"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1996017.1"
                     /translation="MRYRVSENAERDLDEIFCYWAERASPEIADRIIDKITERFWLLG
                     EHPDAGKASDGTAAGVKCFPAGKYLIYYRATRRGTDILHVFHGGRDQRRAFKKVRETQ
                     "
     gene            complement(793..1143)
                     /locus_tag="VGR03_16925"
     CDS             complement(793..1143)
                     /locus_tag="VGR03_16925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEV1996018.1"
                     /translation="MDQMNVSITDRLAGYVRKKVKSGRYNNASEVVREALRRMEDEDA
                     RAVRLAKPSAEDILTDLTEQQLDGIRRRVRAGIESIDTGKYTEYDGREGLKELADGVK
                     ARGRKLLAREDTGR"
     gene            2094..3278
                     /gene="ribB"
                     /locus_tag="VGR03_16930"
     CDS             2094..3278
                     /gene="ribB"
                     /locus_tag="VGR03_16930"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEV1996019.1"
                     /translation="MTENYSYFATVEEAVEEIRQGRMVVLVDDEDRENEGDLAMAAEK
                     ITPEAINFMAKYGRGLICLPLTEDRCDELRLPLMSPINTSVHGTAFCEAIDARRGVTT
                     GISASDRAITILAASDPNTKPQDLARPGHIFPLRARNGGVLVRAGQTEASVDLARIAG
                     LTPSGVICEIMNEDGTMSRVPQLVEFCKEHNLKMLTVADLIRYRMQHERYVRRVAEAV
                     LPTRFGDFRMIAYASDVDHDQHVALVRGDLEGTEPALVRVHSHCLTGDVFGSTACDCS
                     ELVSRSLAAIAKEDRGVFLYLHHTGRGFSVDTAEEPGALPQIHFHKRGQLDREPARQR
                     MVQHESGIGAQILIDLGLKDIRVLTNHPKKVVALEGYGIRIADQVPLSIEAPARPTHQ
                     TL"
     gene            complement(3484..3843)
                     /locus_tag="VGR03_16935"
     CDS             complement(3484..3843)
                     /locus_tag="VGR03_16935"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEV1996020.1"
                     /translation="MAWRSFAIRIPLILCLLFGTGAFAKKKPPAKPVNLNTATSEELQ
                     QVPGIGPATADKILQMRKSYGAFKSVDDLLAIRGLGPKRLEKMRKYLTVGKLAPAKPT
                     TPSAKSAPPAEKPPDKP"
     gene            complement(3903..5276)
                     /locus_tag="VGR03_16940"
     CDS             complement(3903..5276)
                     /locus_tag="VGR03_16940"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV1996021.1"
                     /translation="MATRAVEVAVGVPTLASERAVTWPKRTKARLSNGLEVILAEAHS
                     IPKFHGELFFRSGNAAAMHRAPGLAEMAATVARTGTTKRASRQIEEDLRRIGADLSSG
                     AGADTSAISFGGLSEFAEPLLGMVNELAREASFPEAEFERERRQKVEEVKLERTQPGF
                     LASERLRKVLFGAHPYGQVSPSEAQVAAYKREDLQAVYREFYTPENGMLLLVGDFDAQ
                     EMLKTAEKVFGAWAGKKPATNAAAAPANPRGRRVYLVHVPGAVQTQILAGCHAITRKH
                     PDWVKLGLTNSLYGGAFNSRLVMNIREDKGYTYSPRSGVNALRQHGYFSVSAAVRNEV
                     VAASLTETFYEMDKLRSVPVPVPELADAQNYLSGVFSMGLATQDGLLSQLSTVALNEL
                     PDDYLETYRQKVRALTPQDLLATARKYLDSANMQIVVVGDRAQIEEQAALFGEVEVYE
                     AQGTRLS"
     gene            complement(5276..6589)
                     /locus_tag="VGR03_16945"
     CDS             complement(5276..6589)
                     /locus_tag="VGR03_16945"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV1996022.1"
                     /translation="MKRLQIGMESTKLANGLDVVVAPDSAAPVVTVGVYYKIGFRFEP
                     QGRSGFAHLFEHMMFQGSANAPKMQHIKLVNSSGGVLNGSTSYDVTNYYEAVPSNALE
                     RVLWLEADRMRALKVDDENLKNQRDVVKEEVRVNVMNQPYGGFPWLDMPPVAFRNWAN
                     AHNFYGDFADLDAADLKDVQAFFKTYYVPNNAVLLMLGDVKAEEGLALGKKYFGEIPA
                     GAAPPFADPSEPEQTEERLGNVEEKFGTLPAMAIGYVMPERRTADWCAMALLDQALHG
                     GRAGRMHRELVLEKQVAVEAGGGIDDLFGYNGPTQMTTRILHKPEYSSQATLEAFNAV
                     IREVRENGISADELEQLKVKWRSDYYATLEGGRGGYMPKYGLMHLLACFTLFDNEPQL
                     VNTILDGFLAVTREEIQAVAKKYLRNEKRAIVFRTPVKASAKEAA"
     gene            complement(6586..>7452)
                     /locus_tag="VGR03_16950"
     CDS             complement(6586..>7452)
                     /locus_tag="VGR03_16950"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEV1996023.1"
                     /translation="GRVGRALGRRLRELGWKIGAVVTRSAASARRAVRFIGAGKACAG
                     MTRQILASRVILIATPDDEIAIVAQELARIGAEELRGRVVLHTSGAMGSGALEAARER
                     GAAVGSMHPLQSFSGVAVPSLEGKVFAVEGDTQAVLVARQIARSLGGSPVRIAGNKKL
                     LYHAAAAMAAGHVLAVEEAATQLLFSLGMRRGEAVRALLPLTRQVLENFETLGPRSAW
                     TGPLSRGDYKVVRAHLEALQESPKEFAQAYEALSRLAARVLAQDSAVVLAALEKSFVK
                     EKPKAKAIGGNA"
BASE COUNT         1425 a         2483 c         1920 g         1624 t
ORIGIN      
        1 catatcgttg gagatatcgg tttagctctt cgcggccaca atcgaagccc tcaaccggat
       61 gatcgcgtcg cagtttttcg acgcgcctgg tttcgctcac gggcaagtgc ccgtgtcaaa
      121 gaatccgggt tccttaaaaa gatgttctaa gcggagcagg gggcgcggag gagcatcaag
      181 tgctgccagg aatgctttcc attgcgctgc attcagccca aaagtgcgcc gatccgccag
      241 tgcttcatcg gcgcgggcca gtgcgctctc cagaacgaac tcgcttacgg aacgcttgga
      301 ggctgccgcc gcggctctca aagctgcttt agccttccgt gtcaaccgga gatcgagttt
      361 ttcgctgcgt agcgtacttg atgccatggc cactcctcca tttttgaggc tatctcctcc
      421 gcccgacaat gtcaagacaa gaccgcacgc caccaccacc cgccatttgg cgggtggcag
      481 gcctttactg ggtttccctt actttcttga aagctctccg ctgatcccgc ccgccgtgga
      541 agacgtgcag aatatcggtc ccccggcgcg tggcgcggta gtagatcagg tattttccgg
      601 caggaaagca ttttaccccg gcagcggtgc catccgaagc cttccccgca tctggatgtt
      661 cgccgagcag ccagaaacgc tcggtgatct tgtcgatgat acggtctgcg atctccggac
      721 tagcacgttc cgcccaatag caaaaaatct cgtcgagatc gcgctccgca ttttctgaga
      781 cccggtaacg cattaccgcc cggtgtcttc ccgtgcaagc agcttccgcc ccctcgcctt
      841 caccccgtcc gccagctcct ttaatccctc gcgtccgtca tattccgtgt atttaccggt
      901 atcgatggat tcaatcccgg cccggacgcg gcgccgaatg ccgtcgagct gctgctccgt
      961 caggtccgtt aggatatctt ccgctgaagg ctttgccagc cgcaccgcgc gcgcgtcttc
     1021 atcctccatc cgccgcaacg cttcgcgcac cacctcgctt gcgttgttgt aacgcccgct
     1081 cttcaccttc ttccgtacat agcctgcaag acggtccgtg atcgatacat tcatttggtc
     1141 catggctttg cctcttgttg cagcaccctt ccctccaaat ctatccccgc gcgtcatcaa
     1201 tgtcaatctt tgacatattc ttcccattgc ggctggcagt attctgggaa cgccaatctc
     1261 ccgattggcc tcgtcttaaa tcttaaggat gccaacgttc ccagcgggaa accatgtcaa
     1321 acctgtaacc tgcaaatccg cattttggat cccgggcgca tttgcgggat gcctatggcg
     1381 tttacgccga ccgggtcggg gcatcccggc cgtctaagcc gggacatttc aacgtcttac
     1441 ggctccgttc gaatgatatc gatatactta cttgacatcg attctagttt catgctatag
     1501 ttccgcgtgt ttgccaaacg aatctctcgc tcacccgccg ttccattctt gagccgaacc
     1561 tctgctcccc tccagccctc ccttaagtcc catggaatca tatcgtttac cgacccccac
     1621 cctctaaccc ccatcgaatc atatcgttcc aaaaacatag cgggggaggg ggcacctcta
     1681 gctttcccgg ctggaacgcc aaaaaacccg tcgcgcatct acttttcttt tcaaccactt
     1741 atgaaatgcc ccacgcgcaa ctcctttctt ttgataacca tccattttca tggggggggt
     1801 atggggggtg ttgggttcct tcgacgttca gacggccaaa ccttcagacg tttcaacgcg
     1861 cttccgagct atcctctgtc ttttcacaat cttgcgcact cttttgcgct tttttgcact
     1921 cacgcaaatc tcaactcttc tgttttcaat cgcttccgca ctctgcgtca aaaaacatcc
     1981 ggggtggagg tacccccctc ggtttgcgcc agtcacctgt cacgattcac cagtcactgc
     2041 ctccgcgccg tcaccagtgc tatcattcgg aagaaactta aaactgatta cttatgactg
     2101 aaaactactc ctatttcgcc accgtcgaag aagccgttga agagattcgc caaggccgca
     2161 tggtcgtcct cgtcgacgac gaggaccgcg aaaacgaagg cgacctcgcc atggccgccg
     2221 aaaagatcac cccggaagcc atcaacttca tggccaagta cggccgcggc ttgatctgcc
     2281 tgccgctcac cgaggatcgc tgcgacgaac tccgcctccc gctcatgtcg cctatcaaca
     2341 cttccgtgca tggcaccgct ttctgcgaag ccattgacgc gcggcgcggc gtcaccaccg
     2401 gcatcagcgc ttcggatcgc gccatcacca tcctggcggc cagcgatccg aatacgaagc
     2461 cgcaggacct cgcgcgcccc ggccacattt ttccgctgcg cgcacgtaat ggcggcgtgc
     2521 tcgtccgcgc cggccagacg gaagcgtccg tggacctcgc gcgcatcgcc ggattgactc
     2581 catcaggtgt catctgcgaa atcatgaacg aggacggcac catgtcccgc gtgccgcagc
     2641 tcgtcgaatt ctgcaaggaa cacaacctga aaatgctcac ggtggcggac ctcattcgct
     2701 accgcatgca gcacgagcgc tacgtgcggc gcgtggcgga agcggtcctg cccacgcgct
     2761 tcggcgactt ccggatgatt gcttacgcca gcgacgtgga ccacgatcag cacgtggctc
     2821 tcgtacgtgg cgatctggaa ggcacggagc cggcgctggt gcgcgtccat tcacactgcc
     2881 ttaccggaga tgttttcggc tccactgctt gcgattgcag cgaacttgtc tcgcgctcgc
     2941 tggccgccat cgccaaagaa gaccgcggcg tctttctcta cctgcaccac acgggacgcg
     3001 gcttcagcgt cgataccgcc gaagaacccg gcgcgctccc ccagattcat ttccacaagc
     3061 gcggccagct ggatcgcgaa cccgcgcgcc agcgcatggt ccagcacgaa agtggcatcg
     3121 gcgcgcaaat cctgatcgac ctcggcctca aggacattcg cgtcctcacc aatcatccaa
     3181 aaaaagtcgt cgccctcgaa ggctacggca tccgcatcgc cgaccaggtc cccctgagca
     3241 tcgaagcacc ggcacgtccg acgcatcaaa ccttatgatc cagcacacaa catccacctc
     3301 gatctttcct ggcgcggcaa ggcctctgcc tcgtcgcgcg ctctcgccga cagtcattcc
     3361 gagcgaagca aggaccgctt cctctccgcg cgcattcgcc atctgtcatt ccgagcgcag
     3421 cgagcctcgg cctcgccacg cgccttccca atccgtcatt tcgagcgaag cgagaaatct
     3481 ctcttatggt ttgtctggcg gcttttctgc aggaggagcg gacttcgccg atggcgtcgt
     3541 aggctttgcc ggcgccagct tcccaacggt caagtacttg cgcatttttt caagccgctt
     3601 cggaccgagc ccgcgaatcg ccagcaaatc atcaacactc ttaaatgcgc cgtatgactt
     3661 cctcatctgc aatattttgt ccgccgtcgc cggtccgatg cccggcacct gttgcaattc
     3721 ttccgacgtc gcggtattga gatttaccgg cttcgccggc ggttttttct tggcgaacgc
     3781 gccagtgccg aataggaggc acagaatgag gggaatgcga attgcgaatg atcgccacgc
     3841 catggcggat gtataatgta caaactacgt ctctaaagca ataatagaga cgagtcaggc
     3901 cgctaactga gccgcgtgcc ctgtgcttcg tatacttcaa cttcgccgaa gagcgccgct
     3961 tgctcctcaa tttgcgcgcg gtcgccgacg acgacgattt gcatgttggc ggagtccaga
     4021 tacttgcgcg cggttgcgag cagatcttgt ggcgtgaggg cgcgaacttt ctggcgatac
     4081 gtttcgaggt agtcgtccgg caattcgttc agcgccacgg tcgagagttg cgacagcaag
     4141 ccatcttgcg tcgcgagccc catggaaaag acgccgctca gataattctg cgcgtcggcc
     4201 aattccggta ccggcaccgg cacggagcgc agtttgtcca tttcataaaa agtctccgtc
     4261 agcgatgccg ccacaacttc attgcgtacc gcggccgaaa ccgaaaagta gccatgctgc
     4321 cgcagcgcat tcacgccgct gcgcggactg taggtatatc ctttatcctc gcggatattc
     4381 atgactagcc gggaattaaa tgctccaccg tacaaactgt tggtgaggcc cagcttcacc
     4441 cagtccggat gcttccgcgt gatcgcgtgg catccggcaa gaatctgcgt ctgcaccgct
     4501 cccggcacgt ggaccagata cacccgccgc ccgcgcggat ttgccggagc cgcagccgca
     4561 ttcgtcgccg gttttttgcc ggcccatgcg ccaaacactt tttcggctgt cttcaacatt
     4621 tcctgcgcgt cgaaatcgcc gacgagcagc aacatcccat tctccggcgt gtaaaattcg
     4681 cggtagaccg cctgcaaatc ttcgcgtttg tacgcggcca cttgcgcttc gctcggcgac
     4741 acttgcccgt aagggtgcgc gccgaacaaa actttgcgca atcgctcgct ggccaggaat
     4801 cccggttgcg tgcgctccag cttcacttct tccaccttct gccgccgctc gcgctcgaac
     4861 tccgcttcgg gaaacgacgc ttcgcgcgcc aactcattca ccattcccag caatggctcc
     4921 gcaaattccg aaagcccgcc aaacgaaatc gcgctggtat ccgctcccgc gccggaagaa
     4981 aggtccgcgc cgatgcgccg caaatcttct tcgatctggc ggctcgcgcg tttcgtggtt
     5041 cccgtgcgcg ccaccgtcgc ggccatttcc gcaagccccg gagcgcgatg catcgccgct
     5101 gcgttgccag aacgaaaaaa cagttcgcca tgaaacttcg gaatcgagtg cgcttccgca
     5161 agaataactt cgaggccgtt ggaaagccga gccttcgtcc gcttcggcca cgtcaccgcg
     5221 cgttcggagg cgagtgtggg aacaccaact gctacttcta cggcacgggt tgccattatg
     5281 cggcctcctt tgcgctcgcc ttcacgggcg tgcgaaagac gatggcgcgt ttttcgtttc
     5341 gcagatattt tttcgccacg gcctgaatct cctcgcgcgt gaccgcaaga aatccgtcca
     5401 ggatggtgtt cacaagctgc ggctcattat cgaacagcgt gaagcacgcg aggagatgca
     5461 tcaggccata tttcggcatg tatccgccgc ggccgccttc gagagtggcg taataatccg
     5521 aacgccactt caccttcaat tgttccagtt cgtccgcgct gatgccgttt tcccgcacct
     5581 cgcggatcac ggcattaaac gcttccagcg tcgcttggct cgaatactcc ggcttgtgca
     5641 gaatgcgggt ggtcatttgc gtcggcccgt tgtaaccgaa gagatcgtcg attccgccgc
     5701 cggcctcgac ggcaacctgc ttctcgagga ctagttcgcg atgcattctt cccgcgcgtc
     5761 cgccgtgcag cgcctgatcc agcagcgcca tggcgcacca atcggcggtg cgcctttctg
     5821 gcatcacgta tcctatggcc atcgcgggaa gcgttccaaa cttttcttcc acgttgccaa
     5881 gccgctcctc cgtctgttcc ggctcgctgg gatcggcaaa cgggggcgca gcgcccgcag
     5941 gaatctcgcc aaaatatttt ttgccgagcg cgaggccctc ttccgccttc acatcgccca
     6001 gcatcagcaa cacggcatta ttcggcacgt agtacgtttt gaaaaacgcc tgcacgtcct
     6061 tcaagtccgc ggcatcgagg tcggcaaaat cgccgtaaaa attgtgcgcg ttcgcccagt
     6121 tgcgaaacgc taccggcggc atgtccagcc acgggaagcc gccgtagggc tggttcatca
     6181 cgttgacgcg aacttcttcc tttaccacgt cgcgctggtt tttcagattt tcatcatcca
     6241 ccttcaacgc gcgcatgcgg tccgcttcga gccacagcac acgctccagc gcattcgacg
     6301 gcaccgcttc gtaatagttc gtcacgtcgt aactcgtcga gccgttcagc acgccgccgc
     6361 tggaattgac cagcttgatg tgctgcatct tcggcgcatt cgcggaaccc tgaaacatca
     6421 tgtgttcgaa gaggtgcgca aacccgctgc gtccctgcgg ctcaaaacgg aatccgatct
     6481 tgtagtacac gccgacggta accactggcg cggcggaatc cggcgcgacc accacgtcca
     6541 gtccatttgc cagtttggta ctttccatcc caatctggag tcgtttcacg cgttgccccc
     6601 tatcgccttt gcttttggtt tctctttcac aaaactcttt tccaatgcgg ccaacacaac
     6661 ggcagaatct tgtgcaagta cgcgcgctgc cagacgactc agcgcttcat aggcctgcgc
     6721 aaattctttc ggcgattcct gaagtgcctc cagatgcgcc cgcaccactt tgtagtctcc
     6781 acgcgaaagg ggccccgtcc acgccgatcg cggccccaga gtttcgaaat tctcgagaac
     6841 ctggcgcgtc aagggcaaca aggcgcgcac ggcttcgcct cttctcatgc caagcgagaa
     6901 gagcaattgg gtggctgctt cctccaccgc gagcacatga ccggcggcca tcgccgcggc
     6961 tgcgtgatac aaaagtttct tgtttcccgc gatgcgcacg ggcgatccac cgagcgaccg
     7021 cgcgatctgg cgagccacta ggactgcctg cgtgtcccct tccacggcaa aaaccttgcc
     7081 ttccaaagat ggcacggcaa cgccgctgaa cgattgcagc ggatgcatcg atccgacggc
     7141 cgcgccccgc tcccgcgcgg cttcgagcgc accagagccc atcgctccgc tggtatgcag
     7201 gaccactctc ccgcgcagct cttctgcgcc gatgcgcgcc agctcttgcg caacaattgc
     7261 gatttcgtca tcgggtgtgg cgatcagaat cacacgcgat gccaggattt gccgcgtcat
     7321 tcccgcgcag gccttccctg ctccaataaa ccgaacggct cgccgcgcgc tcgccgcgct
     7381 gcgggtaacg accgcgccga ttttccagcc cagttcgcgc aaccggcgtc ccagcgcgcg
     7441 tcccacgcgt cc
//
LOCUS       DASYVO010000489         1701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_209174, whole genome shotgun sequence.
ACCESSION   DASYVO010000489 DASYVO010000000
VERSION     DASYVO010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209174"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..623
                     /locus_tag="VGR03_16955"
     CDS             159..623
                     /locus_tag="VGR03_16955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996024.1"
                     /translation="MRDLESVGREELIQIILDQNRLIEQLRTEIEQSKHRGSAAPFSK
                     GAHKSNPKTPGRKPGQGCFRFRTPPEQAKKEEPIVVPLAAESCPDCGGALGETRQEIV
                     STTDIPAALVPEVRLYAVEARQCRECGKSVRGQHPDIASGQHGATAHRVGRG"
     gene            620..1519
                     /locus_tag="VGR03_16960"
     CDS             620..1519
                     /locus_tag="VGR03_16960"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1996025.1"
                     /translation="MKALAHILHYVHGVPVRKTAEIVEQLTGVRLTQSAITQDAMRQT
                     EGAVGVHYGELRDTVKQQAVVHTDDTGWRVGGDAAFLMAFVNASLSVYQVRPQHRNEE
                     VRELIPADFEGVMVCDRGRSYDATELEDVAQQKCLAHLIRNAATVAEGKTGRAQQFSE
                     QLKDLLQQAVALSAQKQEMSVEDYWDQAEALHDELTFHLRDRILRDEDNQRLLNGVGA
                     QHDRGHVLRFLGEEEVEPTNNRAERDLRPAVIARKVSHCSKNERGARAFEAFASVLQT
                     IRKTNPIGMVAALVKLISPPAPA"
BASE COUNT          375 a          464 c          559 g          303 t
ORIGIN      
        1 attgcgattg ccgtgggggc aatggtggat gccgctatcg tcgttgtcga acagactcac
       61 aagaaactgg aggagtgggg gtaattgatt agcggctatt cctgtaactc aatttaaaag
      121 ggctggcttt cgaaacaaaa aagctgcaca atgtttgcat gcgggatctt gaatcggtcg
      181 gccgcgagga attgatccag atcattctcg atcaaaaccg gctgatcgag caacttcgaa
      241 ctgaaatcga gcagtcgaag catcgcggct ccgcggctcc gttctccaaa ggcgcgcaca
      301 agtccaatcc caagactccg gggcggaagc cgggtcaggg atgtttccgg ttccggaccc
      361 ctccggagca ggcaaagaaa gaagaaccga ttgttgtacc gctggccgcg gaatcttgcc
      421 cggactgcgg cggagcgctg ggcgaaaccc gccaggaaat cgtttcgact accgatattc
      481 cggccgcgct ggtgccggaa gtgcggctct atgcagtcga ggcgcgccaa tgccgggagt
      541 gcggaaaatc ggtacggggc cagcaccccg acatcgcttc gggccaacac ggggcgacgg
      601 cgcaccgtgt gggccgcggg tgaaagcgct ggcgcacatc ctgcattacg tgcacggcgt
      661 cccggtgcgg aagacagcgg agatcgtgga acaactgacg ggcgtgcggc tgacccagag
      721 tgcgattacg caggatgcga tgcgacagac cgaaggagcg gtcggcgttc attacggcga
      781 gttgcgggac acggtgaaac agcaggccgt tgtccatacg gatgacaccg gctggcgcgt
      841 gggaggcgat gcggcgttcc tgatggcgtt cgtgaacgcg tcgctttcgg tctatcaggt
      901 ccggccccag caccgcaacg aggaagtgcg ggaactgatc cccgccgatt ttgagggcgt
      961 gatggtctgc gaccggggcc ggagctacga cgccacggaa ctggaagatg tggcgcagca
     1021 gaaatgcctg gcccatctga tccgcaatgc cgccacggtg gcagagggga aaaccgggcg
     1081 cgcccaacag ttcagtgagc agttgaagga cctgctgcaa caggcagtgg cattatcggc
     1141 acagaagcaa gaaatgagtg tggaggatta ttgggaccag gcagaagcac tccatgatga
     1201 actcaccttt cacctgcgag accggattct ccgcgacgaa gacaatcagc ggctgctgaa
     1261 cggcgtgggt gcgcagcatg atcgggggca cgtgttgcgg tttctcggcg aggaagaagt
     1321 cgagccgacc aacaaccggg cggaacggga tctgcgcccg gcggttatcg cgcgcaaggt
     1381 ttcgcattgt tcgaaaaacg aacgtggcgc cagggcgttc gaggccttcg cgagtgtgct
     1441 ccagaccatt cgcaagacga atcccatagg catggtggcg gcgttggtaa aactgatcag
     1501 ccctcccgcg ccggcttgac agccgcgcaa acaccaatat tccggctatc attcgaagcg
     1561 accttcaggg cgtttcccct gttgcgtccc ggatgtcgct aatcaattac gacggattca
     1621 tatcgtaagc ccggaagcaa tcgcttgaaa gcctgggtca ggcgggccac tacggcgtat
     1681 tgcgcgggtc tgacttcgag g
//
LOCUS       DASYVO010000490         8766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_150741, whole genome shotgun sequence.
ACCESSION   DASYVO010000490 DASYVO010000000
VERSION     DASYVO010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150741"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VGR03_16965"
     CDS             <1..369
                     /locus_tag="VGR03_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996026.1"
                     /translation="LKPLESWGANNHTYDASFGTDNFDFMLEGVPTLVANQEEANYLP
                     NYHAASDTLDKVDMRELKLHTVLAALTAWGIADRAEPLGKRLSRAELDVLVKETGLDQ
                     QLKLLGYWDAWQSGARGRKP"
     gene            complement(366..1388)
                     /locus_tag="VGR03_16970"
     CDS             complement(366..1388)
                     /locus_tag="VGR03_16970"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV1996027.1"
                     /translation="MYRVSYFGQCVLSMLALVVCARGARAQEVNCQRRTLPVALRDKL
                     NLPIQGISAADFEAKVHGKPIKILSLAPDPRPHRLVLILDTSGSMGSTASDSPLITLE
                     FALARHFFDVNRQRSQIALLMFNNDVTDVVDFARGNTAVGDKLQQIASDHNFVKTNVK
                     GRTALRDAILEGLQLLDHPSSADALFVLTDGGDNASKRKSADVTQRLAVTSVRLFAVV
                     LHQEVSYRNRTPEELSGPGELSEIAGKSGGEILSAAAWHGKQIALSADAEAKLKTQET
                     LGRLYETILQDNLLEIELPFPIAKNEHWELKLSDAARRQWKGTQITYPTTLISCNSEV
                     SGSGRR"
     gene            1493..2014
                     /gene="ruvX"
                     /locus_tag="VGR03_16975"
     CDS             1493..2014
                     /gene="ruvX"
                     /locus_tag="VGR03_16975"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV1996028.1"
                     /translation="MGSARNITRFTVDKAGRAAILAVDYGRARIGLALADSETRMAQP
                     LSTMERINRNEDMRRLRELVREQGVKQIVVGLPLRLDGSRGEMAEEAERFAQRVRKQI
                     GIPVEMVDERLTSWEAERLMEEIQGRFIRDEKLAGGKKPKNAQAKMTVDAVAAAVILK
                     EYLDRQGQTADKA"
     gene            2047..3144
                     /gene="mltG"
                     /locus_tag="VGR03_16980"
     CDS             2047..3144
                     /gene="mltG"
                     /locus_tag="VGR03_16980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV1996029.1"
                     /translation="MKFLLALLLLIVLIAGGAAAWIWYGITKPYQSFAADGAFVDVPR
                     GASSRHVANLLKTNGVVRSALALEIYARRHPKRTLQAGEYFFDHAMTGREVFWKLANG
                     QVYQQPFTVREGETMFDIARELQAGKFMPAGDFLFAAGDPALIRDFAPGVRTLEGFLF
                     PATYGLPRHPSASELTTEMVHKFKEEWKRIASPAAVSPADEGGHRNVNRIVTLASLVE
                     RETPEPEERPLVAGAFENRLDKGMRLQCDPTVIYGMERLGKYNGSLTGKDLSFDSLYN
                     TYEHGGLPPGPIGNPGEASLRAALQPARTDYLYFVANTQGGHFFSATLAEHNKNVVKY
                     RRLLAGLPADPPPPPPSPQNKHSKQHRGTAR"
     gene            3141..3698
                     /locus_tag="VGR03_16985"
     CDS             3141..3698
                     /locus_tag="VGR03_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996030.1"
                     /translation="MSEDLKTKKALILETAREIKVQQWTPAEIDQLRRRLVAEHGEAG
                     KTGMEYIADVLKDAGQKVTINQQEEAEEQYEEEFDDLLHFKTLEDAEVSIMRLDELMR
                     KFRGQGEHAAEERVLNIARLGKRRAEMISRNSKVEAHKRAEKAEIAVWFRIWLETPDA
                     FFDWLDVRKQTPEFRAKFPQLEPEE"
     gene            3695..4297
                     /locus_tag="VGR03_16990"
     CDS             3695..4297
                     /locus_tag="VGR03_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996031.1"
                     /translation="MNTPGIGAPGSGALELPPFSLDVQALDLGAGVKAVGLALLETEN
                     REPVRGKDAAEIWSAVFPALAGTASYVADFFSHIERVREFCKMREIALRDAAERCAVL
                     PQPNQEQLRQIFERFEGETFGVRAGAASQSADAALEGDLSKRGMDAYQAAYARYTFCA
                     ICEPEDGWVTLLSESLWPSEVIRRVRPAVQPFDIHIARPN"
     gene            4389..5303
                     /locus_tag="VGR03_16995"
     CDS             4389..5303
                     /locus_tag="VGR03_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996032.1"
                     /translation="MRRVKTCLLLATLAAISCGCGGGRVVTGRTVVAQKPVAKDATRE
                     ELLEAYNLIARGTKTLNATVELKPTAGSKYSGVIDEYHEVKAFLLAARPAEIRVIGQA
                     PVIGTTVFDMASDGDTFRVSIPSKNKFLVGPVALERASSKPIENLRPQHLLDALLWPE
                     IRKEEAVILREFNDENARYYILTVLRGGYQVEVLREVWFDRADLQVARMQTFGPKGVL
                     LSDVRLADWQPPDSAAGQSAPVTLSSGAASFPRAIRIERPHDDYKLDLQVTKVSFNEE
                     IPAERFKLEQPAGAELVPVGDATENKQP"
     gene            5300..6397
                     /locus_tag="VGR03_17000"
     CDS             5300..6397
                     /locus_tag="VGR03_17000"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1996033.1"
                     /translation="MMGELIVRNLLHRPLRTLIGAMGIAVEVALVVLIVGLTSGLLTE
                     TARRIEGIGADIMLQPPSSSIFFALGSAPMPIKIGDKIGELKYVRSVAPVLFQFNTSG
                     IEIVYGIDQESFRAVSGGFVFLQGHDLEGPDDILVDDVAAKTKKIQPGQTYRIFDHDW
                     HVAGIVEHGKGSRLFVPLATLQELVGAREKATIFFVKCTRPEHTEDVMEDMRHLLPNY
                     TVRPLKDYLSLMTSTNIPGLESFINAMILLAITIGLLVIFLTMYTTVIERTRDIGVLK
                     SLGANHWFVIRALLSESAALCVLGIGAGIGLSYLVRAGFLWKFPNLTILITSGWILRA
                     AGIALLGALFGASYPAWLASRKDAVEALSYD"
     gene            6492..7238
                     /locus_tag="VGR03_17005"
     CDS             6492..7238
                     /locus_tag="VGR03_17005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1996034.1"
                     /translation="MGIAATIPELAEGLIFQGKKSVEPILRTENLWKLYRAGKVEVPA
                     LRGVNFEVQPGESVAVMGPSGCGKSSLLYVIGGLAQASRGKVLVDGNDLTELSDAART
                     KLRRHKIGFVFQRFNLLPTLTAKGNIAIAQFIHGDGFDPHRFDVVTQMLGLAGRLHHK
                     PSELSGGEQQRVAIARAIINEPKILLADEPTGNLDSKNSEIVLQMLRQLNKDLGQTVV
                     MITHNPEATTYFDRVVHMRDGVIVDGVPAD"
     gene            7240..7626
                     /locus_tag="VGR03_17010"
     CDS             7240..7626
                     /locus_tag="VGR03_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996035.1"
                     /translation="MASLGQILSRTFFWSYERGTWQYDVAVIMILVFVLLTPRNWFRD
                     KPQSGARVTPGQVQLISQEGNRLKYRVDARILAPPTKLALQNDLHTALQEALPELHNG
                     NFSIANIEPLRDDQGTVIAYQVEIHH"
     gene            7758..8462
                     /locus_tag="VGR03_17015"
     CDS             7758..8462
                     /locus_tag="VGR03_17015"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEV1996036.1"
                     /translation="MRRPPFLLFALVSLCCAAVWPQTLAAQTTSSWTLDGVLVMLDKS
                     AQDFHTLTADIEHIKYTAVVKDTSTETGQIFVRRDEKMRIDFQKPDLRTILRNGDSLF
                     VFTPKINRVEEYNIGKHRSFVDQYLALGFGTKGDTLKKNYVLTLIGEEEFDSRKTVVL
                     ELTPKSDQARSQISRIRMWIDEASWLPVQQKFFETSSEDYFLSHYTHVMKNLKIGDAK
                     FKPDWPKSVKVEKPRG"
     gene            complement(8532..>8766)
                     /locus_tag="VGR03_17020"
     CDS             complement(8532..>8766)
                     /locus_tag="VGR03_17020"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV1996037.1"
                     /translation="QELQRIPRGRTRTYSQVARSLGQPKAVRAVARACATNPVSIVVP
                     CHRVIREDGTLAGYRWGLSRKEQLLAQERAAGG"
BASE COUNT         1916 a         2468 c         2661 g         1721 t
ORIGIN      
        1 ctcaagccgc tggaatcctg gggcgcgaac aatcacacgt atgacgcttc gtttggcacg
       61 gacaatttcg atttcatgct cgaaggcgtg cccacgctcg tggcgaatca ggaagaggcc
      121 aactacctgc ccaattatca cgccgcctcg gacacgcttg acaaagtcga catgcgcgag
      181 ctgaagctgc ataccgtgct cgctgcgctg acggcctggg gaatcgccga ccgtgccgaa
      241 ccgctcggta agcgcctttc gcgcgcggaa ctcgatgttc tcgtcaaaga aaccggcctc
      301 gaccaacagc tcaaattgct tggctattgg gacgcgtggc agagcggcgc gcgcggccgc
      361 aagccctagc ggcggcctga gccggaaacc tcggaattgc aactgatcaa tgtcgtggga
      421 taggtgatct gcgtgccctt ccactgtcgg cgagcagcgt ccgaaagttt taattcccag
      481 tgctcgttct tcgctatggg aaaaggaagc tcgatctcca agagattatc ttgaagaatc
      541 gtttcgtaaa gacgtcctag agtttcttgt gttttcaact tggcctcggc gtcagcactt
      601 agagcgatct gtttaccgtg ccacgcagcg gcgctgagga tttctccccc ggacttcccg
      661 gcgatctctg ataactcgcc cggaccggac agctcctcgg gagttcggtt gcgataactg
      721 acttcctggt gaagaactac agcaaagagc cgcacagaag tcacggcaag gcgctgcgtt
      781 acgtccgcag atttacgctt gctggcgttg tcaccgccgt cggttaaaac gaacagagca
      841 tctgcggaac tgggatgatc cagcaattgt agcccctcca agatggcatc gcgaagagca
      901 gttctgccct tcacgtttgt ctttacgaag ttgtggtcgc tggcaatttg ctgcagcttg
      961 tcgccaactg cagtatttcc ccgggcgaaa tcaaccacgt cggtaacatc attattaaac
     1021 atcagcaagg cgatttgaga tctttggcgg tttacatcga aaaaatgccg agcgagcgca
     1081 aattccaacg taatgagcgg actgtcacta gccgtgctgc ccatgctccc gctggtgtct
     1141 aggattagaa cgagccggtg cgggcgcggg tcgggtgcga gcgaaagaat cttgatgggc
     1201 tttccgtgaa ccttcgcctc aaagtcagca gccgaaatgc cctggatcgg aagattcagc
     1261 ttatcgcgca gggcaactgg caaggtgcgt cgctggcagt taacttcctg cgcccttgcc
     1321 cctcgtgcac agaccaccag agcaagcatg ctgagtacgc attgcccgaa ataactaact
     1381 cggtacatag gtaagccaat ctaatatcac gtgcgccgga ctggatgcaa cgtccggtag
     1441 tgctcaggaa tctgtcgaag aggggcgcat cggttcaagc tatactgact caatggggtc
     1501 agcgagaaat attacccgct ttacggtcga caaagccggc cgggccgcga ttctggcggt
     1561 ggattatgga cgggcgagga tcggacttgc tctcgcggac tcggagacgc ggatggcgca
     1621 accgctgagc acgatggagc gcatcaaccg gaacgaggac atgcggcggc tgcgggaact
     1681 ggtacgcgaa caaggcgtga agcagatcgt cgtgggactg ccgctgcggc tggatggttc
     1741 ccgcggcgaa atggccgagg aggcggagcg ctttgcgcaa cgcgtgcgga aacagattgg
     1801 cattccggtg gaaatggtgg acgagcggct caccagctgg gaggccgagc gcttgatgga
     1861 agaaattcag ggaaggttca ttcgcgacga aaaactggcc ggcggcaaga aaccgaagaa
     1921 cgcgcaggcc aagatgaccg tggacgccgt ggcggctgcg gtgattttga aagagtacct
     1981 ggatcggcaa gggcagacgg ccgacaaggc ctgagcgaac taagaaagcg actcggtgag
     2041 gctcaattga agtttttgct cgcgctattg ctcttaatcg tgctgattgc cggaggcgcc
     2101 gcggcatgga tctggtacgg catcacgaag ccataccaga gctttgcagc ggatggcgcc
     2161 ttcgtcgatg tgccacgcgg ggcttcgagc cggcacgttg cgaatctgct aaaaacaaat
     2221 ggcgtggtgc gcagcgcgct ggcgttggag atttatgcgc ggcggcatcc gaagcgcacg
     2281 ctacaggccg gcgaatattt tttcgatcac gccatgaccg gccgcgaggt tttctggaaa
     2341 ctcgcgaacg gccaggtgta ccagcagccc ttcacggtgc gcgaaggcga aacgatgttc
     2401 gacattgccc gcgaactgca agcgggaaag ttcatgcctg cgggcgattt tctttttgcc
     2461 gcaggtgatc cggcgctgat tcgcgatttt gcgccgggcg tacggacact cgaagggttc
     2521 ctcttccccg ccacctatgg acttccgcgg cacccatcgg ccagcgaatt gaccacggaa
     2581 atggtgcaca agttcaagga agagtggaag cgcatcgcct cacccgccgc ggtgagccca
     2641 gcggacgaag gcggccaccg gaacgtaaac aggatcgtga cgttggcgtc gctcgtggag
     2701 cgggaaaccc cggagccgga agagcgaccg ctggtagcag gcgcgtttga aaaccgcctg
     2761 gacaaaggca tgcgcctgca atgcgacccg acagtgattt acgggatgga gcggctcgga
     2821 aaatacaacg ggtcgctgac ggggaaagac cttagcttcg attcgctgta caacacgtac
     2881 gagcacggcg gtcttcctcc cgggccaatt ggaaatcccg gcgaagcgtc gctgcgcgct
     2941 gcgctgcagc ccgcgcgaac ggattacctc tatttcgtgg ccaacaccca gggcggacat
     3001 ttttttagcg cgacgctggc ggaacacaac aagaatgtgg tcaagtaccg tcggttgctg
     3061 gcaggtctgc cggcggatcc accgccgcca ccgcccagcc cccagaataa acacagcaag
     3121 caacaccgag gcacagcccg atgagcgaag acttgaaaac gaagaaggcg ctgatcctgg
     3181 agaccgcccg ggagatcaaa gtacagcaat ggacgccggc agagatcgat cagttgcggc
     3241 gacggctggt ggcggagcat ggcgaggccg gcaaaaccgg aatggaatac attgcggacg
     3301 tactgaaaga cgcgggacaa aaggtaacca tcaaccagca ggaagaagcc gaggaacagt
     3361 acgaagaaga gttcgacgat ctgctgcact tcaagacgct ggaagatgcc gaggtcagca
     3421 tcatgcggct ggatgaattg atgcgcaagt ttcgcgggca gggtgaacac gcggccgagg
     3481 agcgcgtttt gaacatcgcg cggcttggca agcgccgcgc cgagatgatc tcgcgcaaca
     3541 gcaaggtgga ggctcacaag cgcgccgaaa aagcggagat cgccgtctgg ttccgcatct
     3601 ggctcgaaac gccggatgcc ttcttcgact ggctggacgt tcggaagcag acgccggaat
     3661 ttcgcgcgaa gtttccgcaa ctggagcccg aagaatgaat acgccgggta ttggcgcgcc
     3721 aggaagcggt gcgctggaat tgccgccgtt ttccctggat gtgcaggcgc tggacctcgg
     3781 cgccggtgtg aaagcggtgg gactcgcact gcttgaaacg gaaaatcgcg agccggtgcg
     3841 aggcaaggat gctgcggaga tctggtcggc ggtcttccct gcccttgcag ggaccgcatc
     3901 gtacgtcgcg gattttttca gccacatcga gcgcgtgaga gagttttgca agatgcggga
     3961 aattgcgttg cgtgatgcgg cggagcgctg cgccgtgctg ccgcagccga atcaggaaca
     4021 attgcggcag atctttgaac gattcgaagg cgagacattt ggcgttcggg ccggcgcggc
     4081 gtcgcaatcc gccgatgccg cgcttgaagg cgacctgtcg aagcgcggta tggacgccta
     4141 tcaggcggca tacgcccgct acacgttttg cgccatttgc gagccggagg acggctgggt
     4201 gacgttgctg agcgagtcgc tctggcccag cgaagtcatc cggcgggtgc ggcccgcggt
     4261 tcagccattc gacatccaca ttgcgcggcc caattgaacg tctccccggc tgcggcctgc
     4321 gttgattgac cggcatccga gtggttgcta gattgcttca tttgtaagag tgaggacgga
     4381 actgattgat gcgacgtgtg aaaacgtgcc tgctcctggc gacactggcg gctatttcct
     4441 gcggctgcgg aggcggaaga gttgtgaccg gccgcaccgt agttgcacag aagccggtcg
     4501 caaaggatgc cacgcgtgaa gaactcctcg aggcatacaa tctgatcgcg cgcggcacaa
     4561 aaacgctgaa cgccaccgtg gagctaaagc cgaccgcggg atcgaagtac agcggtgtca
     4621 tcgatgagta tcatgaggta aaggcgtttc ttctggcggc gcggccggcg gaaattcgcg
     4681 tgatcgggca ggcaccggtg atcggaacca cggtgttcga catggccagc gacggcgaca
     4741 cgtttcgggt ttcgattccc tcgaagaaca aattcctcgt tgggccggtg gccctcgagc
     4801 gcgccagcag caagcccatc gagaatttgc ggccgcagca tctgctggat gcgctgctct
     4861 ggccggaaat ccgcaaggaa gaagcggtga tcttaaggga gttcaacgac gagaacgcca
     4921 gatactacat cctgaccgtt ttgcgcggcg gttatcaagt agaagttctg cgggaagtct
     4981 ggtttgaccg cgcggacctg caggtcgcgc gcatgcaaac tttcgggccg aagggtgtcc
     5041 tactttcgga tgttcgcctg gctgactggc agcctccgga cagtgccgcc gggcagagtg
     5101 cgccggtcac cctatctagt ggcgcggcct cgtttccgcg tgcgattcgg atcgaaaggc
     5161 cgcacgatga ttacaagctc gatttgcagg tcacgaaagt cagcttcaac gaagagattc
     5221 ctgccgagcg atttaagctc gaacaaccgg cgggagcgga attggtgccc gttggcgatg
     5281 ccacggagaa caaacagcca tgatgggtga gctgatcgtc cgcaacctac tgcaccgccc
     5341 gctgcgcacg ctgattggcg caatgggaat cgcggtcgag gtcgccctcg tcgtcctcat
     5401 cgtcggccta accagcggtt tgctgaccga aacggccagg cgcattgaag gcatcggcgc
     5461 agatatcatg ctgcagccac catcctcgtc tatctttttc gccttaggca gcgcccccat
     5521 gcccatcaag atcggcgaca agatcgggga actcaagtat gtgcgatcgg tggcccccgt
     5581 gctcttccag ttcaacacct caggaataga aatcgtttac ggaattgatc aagagagctt
     5641 tcgggcggtc tcaggcggat ttgtgttcct tcaagggcac gacctggaag gtcccgacga
     5701 cattcttgtg gacgacgttg ccgcgaaaac gaaaaagatt cagccggggc agacctaccg
     5761 catcttcgat cacgactggc acgttgcggg aatcgtagag cacggtaagg gctcgcgcct
     5821 ttttgtgcct ctcgctacgc tccaggaatt ggtgggcgcg cgggaaaagg ccaccatctt
     5881 ctttgtgaag tgcacgcggc ccgaacacac cgaagatgta atggaagaca tgcgccacct
     5941 ccttcccaac tataccgtcc ggcccctgaa ggattacctc tccttgatga cctcgacaaa
     6001 tatcccgggc ctggagtctt ttatcaacgc gatgatcctg ctggccatta ccatcggcct
     6061 gctggtcatt tttctcacca tgtacactac ggtgatcgag cgcaccaggg acatcggcgt
     6121 tctgaaatcg cttggcgcca accactggtt tgtcatccgg gcgctgctct cggaatccgc
     6181 ggcgctttgc gtcctcggaa taggcgcggg catcggactc agctacctgg tgcgggccgg
     6241 gtttctctgg aagtttccaa acctgaccat tctcatcacg tcgggatgga tccttcgcgc
     6301 ggctggcatc gcgctcctgg gcgcgctgtt cggcgcgagc tatccggcgt ggctagcgag
     6361 ccgcaaagat gccgttgaag cgctgtcgta cgactaggac gcatagaata gggcgcggga
     6421 cgcgccgtga acgtagcgga cactaagaag tgaggaaagg gccgcgaagc aggcgggatg
     6481 gctaggcacg aatgggaatc gcagctacaa taccagaact cgccgaaggg ctgattttcc
     6541 agggaaaaaa gagcgtggag ccgatactca gaacagaaaa cttgtggaaa ctctaccgcg
     6601 cgggcaaggt ggaagtcccg gcattgcgtg gtgtgaattt cgaggtacag cccggcgaat
     6661 cggttgccgt gatggggcct tcgggctgcg gcaagtcttc gctgctgtat gtaatcggcg
     6721 gcctggcgca agcctcccgc ggcaaggtgc tggtcgacgg gaatgacctc accgaactga
     6781 gcgacgccgc gcgcactaaa ctgcgccgcc acaagatcgg cttcgtcttc cagcgtttca
     6841 atctgctccc cacattgacg gctaagggca atatcgccat cgcacagttt attcatggcg
     6901 acggtttcga cccccaccgc ttcgatgttg taacgcagat gcttggtcta gcgggacgtc
     6961 tgcaccacaa gccttcggaa ctttcgggcg gcgaacagca gcgcgtggcc atcgcgcggg
     7021 cgatcatcaa tgaaccgaag attctcctcg ccgacgagcc cacgggaaac ctggattcga
     7081 aaaattcgga aatcgtgctg cagatgctgc ggcaattgaa caaagatcta ggccagaccg
     7141 tcgtcatgat cacacacaac cctgaagcca ccacgtattt cgaccgcgtg gtgcacatgc
     7201 gcgatggggt tatcgtcgac ggggttcctg cggattgata tggcaagcct tggccaaatt
     7261 ctttcccgga cgtttttctg gtcctacgag cgcggcacgt ggcagtatga cgtcgccgtc
     7321 atcatgattc tggtcttcgt gcttctcact ccgcggaatt ggttccgcga taaaccgcaa
     7381 agtggagcgc gggttacacc ggggcaggtc caactaattt cgcaagaagg gaaccgcctg
     7441 aagtatcgtg tggacgcgcg catcctggct cccccgacaa agctcgcact ccagaacgac
     7501 ctgcacaccg cgttgcagga ggcgctgccc gagctacaca atggcaattt ctcgatcgcc
     7561 aacatcgaac cactgcgcga cgaccagggc actgtgattg cctaccaggt ggaaattcac
     7621 cattagccgc cgctcttacc ggtggcaaga tcttgtttcc ggggtacccc tgtggcgcgt
     7681 tcgcgcatct ctttgtatat gttaggattc caagtgcttg tatatgctgc cgcagccttg
     7741 ctggatagag gccacgtttg agacgtccac cttttcttct attcgccctg gttagtctgt
     7801 gctgcgccgc tgtctggcct caaacgctcg ccgcgcagac aacttccagt tggaccctcg
     7861 atggcgtcct ggtaatgttg gataagtccg cgcaggactt tcataccctc acggccgata
     7921 tcgagcacat caaatacact gcggtagtga aggatacttc gaccgagacg ggccagatat
     7981 tcgtgcggcg cgatgaaaaa atgcgcatcg atttccagaa gcccgatctg cggaccatac
     8041 tccgaaacgg cgattcgctc ttcgttttca ccccgaagat caatcgcgtt gaggaataca
     8101 acatcggaaa acaccgttcg tttgtggacc agtatctggc gctgggtttc ggaacgaaag
     8161 gcgatacgct caaaaagaac tacgttctga cgctgatcgg agaggaagag ttcgacagcc
     8221 gcaagaccgt cgtgctggag ctgacgccaa aatccgacca ggcacgcagc cagatctcca
     8281 gaattcgaat gtggatcgac gaagcatcct ggctgccggt acagcagaaa tttttcgaga
     8341 cgagctccga ggactacttc ctgtcccatt acacccacgt aatgaagaat ctgaagatcg
     8401 gcgatgcaaa gttcaagccc gactggccaa aaagcgtaaa agttgagaag ccccgagggt
     8461 aatttctagc ctactgcaat ctgctgattc tcgcggactc gctggttttc atacacttaa
     8521 aagaccataa gctagccacc agcggctcgt tcctgcgcca gtaattgttc cttgcgagaa
     8581 agcccccaac gatagcccgc gagcgtgccg tcctcgcgaa taacgcgatg gcacggtacc
     8641 acgatggata ctgggttcgt ggcgcaagct ctcgccaccg cccgcacggc cttgggctgg
     8701 ccgagggacc gcgcgacttg cgagtacgtg cgcgtcctgc cgcgcggaat ccgctgcagt
     8761 tcctgc
//
LOCUS       DASYVO010000491        16804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_82646, whole genome shotgun sequence.
ACCESSION   DASYVO010000491 DASYVO010000000
VERSION     DASYVO010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 16804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16804
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82646"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VGR03_17025"
     CDS             complement(<1..249)
                     /locus_tag="VGR03_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996038.1"
                     /translation="MISRRFALAFLFVLFVLCTSVRLFAGPDAPKQFDPKLFQELRWR
                     LIGPARGGRALAVAGVRGQPEVFYFGSVGGGIWKTNDAG"
     gene            complement(373..1707)
                     /locus_tag="VGR03_17030"
     CDS             complement(373..1707)
                     /locus_tag="VGR03_17030"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV1996039.1"
                     /translation="MAATFFMVSGGTYGTEEIIHGAGYGHGILILLFLPVLWCLPTAF
                     MIGELSSALPQEGGYYAWVRRGLGNFWGFQEAWLSLAASIFDMAIYPTLFVFYLKQMS
                     PWFGVGNHGIYAGLFVVVTCAVLNLAGIRVVGITSLWLFFLLSAPFALIVVMAPLKMG
                     ALAEGHAAPAATGLGLVGGVLVAMWNYMGWDNASTIAQEVERPQRTYPKAMIAAVVLV
                     ALTYVLPFVAVYFAGIPASTFGSDGAWAEVAGLVGGKFMGFEWLRFLIVLGGMMSAFG
                     MFNALVLSYSRLPLAMARDGMLPKFFGKMSARTQAPWVAIIFCATCWALCLGLGFKRL
                     VTLDIMLYGISLMLEFVTLVALRIREPKLKRQFRVPGGLAGAITCGIFPLLLLSLAMV
                     ESNHETVLGMNGLLFGVLIIMSGFLIYFVTGKLKIRQTKPVVMEEIEELETA"
     gene            2081..4207
                     /locus_tag="VGR03_17035"
     CDS             2081..4207
                     /locus_tag="VGR03_17035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV1996040.1"
                     /translation="MKFSFRCSALCAVLVLAPLALAQQPAPRAITIDDYFQIREVRDP
                     QLSPDAKWVTYTVKTALLKEDKNEERIWMVPAAGGAPLALTAEGVSSSHARWSPDGKF
                     IAFLSARGEDKSQVWLLNRHGGEAERLTDTPQDVEDFAWSPDSRQLVMILRDASPEEL
                     EAAKENKNKGVAHAATSGGSDAKDKKKAQKPWVIDRLQFKLDEVGYLDRRRTHLYVFD
                     LAAKALTQITSGDYDDSEPAWSPDGKRLAFSSNRSTPDPDAASDQNIWVVAANNPDIG
                     AHLTQVTTNPGADGSPAWSPDGKWIAYVTQLDPHLFDYATKHLAVAPATGGEAQVLTL
                     ALDRMVIVPRFSPDGKFIYFIADDDGTQNLCRIPVTGGEITRPVAGRLMVNDYSLAKT
                     GDLVAQVATLDRPDELYTIPDGKLTQITHTNDALISQLKISQSEYVHFKSKDGTIVSG
                     YLYKPLDYVPGKKYPTILRPHGGPVWAYYAEFDHLPQLYAANGYAVLFPNPRGSTGYG
                     QDFAKAIWADWGNKDFQDDMAMVDYAIELGITDPEKLAVGGWSYGGISTDFIIAQTNR
                     FKAAISGAGSALFISSYGHDHYQKDYETELGHPWENKAVWERVSPFYRVANITTPTLF
                     MGGEIDWNVPILGGEQMFQALKRLGRTTELVVYPGEYHEFKTPSHIKDRLERYLAWYA
                     HYVKGDSAPARPPEPPPATPPAKPAN"
     gene            4373..5425
                     /locus_tag="VGR03_17040"
     CDS             4373..5425
                     /locus_tag="VGR03_17040"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEV1996041.1"
                     /translation="MRNRFSVVAALLLTPFLAHSQTRFTVGTASAAPGEKSTGTLEVS
                     AGVDAATSIPVVVVNGAKPGRVLALVSGAHGTEYVSIIAIEKLIAALDPAQVTGTVIL
                     VPLINIQSFEQKVPHVNPIDNKSMNRFYPGKADGTQTDRASYLITKEIVDRCDYLIDY
                     HGGDLDESLRPYAYWAPTGREAQDRTSKEMVLAFGLDHIIIWRERPTDPAVTRYLDNT
                     ASVRGKPSIVVEAGHAGTVESDDLALLVNGTLSTMRALKMLPGDPHPIENPVWIDKLV
                     DVLADGPGIWYPLVKRGTYVSAGMKLGTITDYVGKVIAEPRAPVSAVVLHLNALPSLK
                     KGDNIADLGVVAPHAP"
     gene            5586..6281
                     /locus_tag="VGR03_17045"
     CDS             5586..6281
                     /locus_tag="VGR03_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996042.1"
                     /translation="MRKWGIVISVFYALILLGLIVPGSMFIAGGDFSKWSGLLPGMEG
                     TYTDLTFWILAGAILASQALLLFLSVDTSHKRLKPRAHILVSCAVVAVLTALLTSAVI
                     WSLGVAVRGDKFFHFFFDFAHGFFDEDNHLLIFWGGLWLLWGILFYFYFRNSSAVVTK
                     IISWLLKGSVLELLIVVPCHIIVRRRHDCSAPLATSFGLATGLAIMLLSFGPSVLFLY
                     RKRLDSYLPRSSV"
     gene            6724..7173
                     /locus_tag="VGR03_17050"
     CDS             6724..7173
                     /locus_tag="VGR03_17050"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEV1996043.1"
                     /translation="MSENNKAIVRRLIEELWNKGNLSLADQLFAPNYEHHDASSPDFG
                     RGPESEKKRATLYRTAFPDIRLTIEDIIAEGETVMTRWSCRGTHKGDLSGIAPTGKEF
                     NITGVTIARLSNGKLAEGYVNWDALGLMQQLGVVPELAKAKAAAAPR"
     gene            7383..7685
                     /locus_tag="VGR03_17055"
     CDS             7383..7685
                     /locus_tag="VGR03_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV1996044.1"
                     /translation="MSQSTPMERTFHVYLMASKSGVLYLGVTGNLSHRVGQHKEKSVS
                     GFTQKYNITKLVWFEPHTSIRSAISHEKEIKKWRRSKKIALIESLNPEWNDLSLNL"
     gene            8240..8884
                     /locus_tag="VGR03_17060"
     CDS             8240..8884
                     /locus_tag="VGR03_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996045.1"
                     /translation="MENESNAGLRHLLDLARNENNRQRPDLALGYLQEARSEIERREG
                     EAIWAEHHLLLAEAYAAKCDPAAEPLFEELFDRLRKLPERHPELELLAHEHFADFLVR
                     LDGRRSVARDHFEQARKLAIEENLREESARTELKLTKLHLEMDSDPELPNFKSFRRVA
                     KNGGYTYQVQLASWQRHYLGQQGLNKGLRYARKRNVASDGYFADILESVKKSTE"
     gene            8881..9627
                     /locus_tag="VGR03_17065"
     CDS             8881..9627
                     /locus_tag="VGR03_17065"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEV1996046.1"
                     /translation="MKSKTNPYMVRISSLVGEANQNLAVRDFVQSHRQQGESLESVAT
                     RLGIAEIKYEPLPFDGGIFEDGATRVIKINSFRPEVRRNFTLAHELAHLIMAASFEEK
                     KRKPCPDDPNLERTCDSIAAELLMPYEETVRLATQLGRQSPEKLGTLAKKFGVSLETA
                     ARRLHTELGLWKLPMGLWECGPVARELWFVGKRPWRSPRQSFSAFELARESRIPIVTR
                     ERYPRGTHTELVDLKVLHIGNNKILGVIAI"
     gene            9817..10212
                     /locus_tag="VGR03_17070"
     CDS             9817..10212
                     /locus_tag="VGR03_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996047.1"
                     /translation="MEDKSPVSKIFSQLGISKSMQRIKFGGVVGKQTLLGVIGSATLV
                     SIAWGADATTKLVIAVLAGVLLILVALLNFSFANKHAAEAMLEGTEMLAFQHQVHAAK
                     SAEVPQNAPVIPNPGGSAPQLNPSQETEQ"
     gene            11029..11568
                     /locus_tag="VGR03_17075"
     CDS             11029..11568
                     /locus_tag="VGR03_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996048.1"
                     /translation="MNTTVASPNFTPDPVAVPPKSTSPRCPYLYPNGKRCSLPGSQVH
                     SGSCLRHGQPNAPTAIPVPVQNDSEDLSPELLPELSEFESAIDINKFLARLLVLVTKG
                     RISPRRASVLGYITNQLLHSHRAIDRENFLQLEDAGPPRVEIGDIPRPARSQPNRNAG
                     PTIIWDVPGVSIDREKTPS"
     gene            11792..15016
                     /locus_tag="VGR03_17080"
     CDS             11792..15016
                     /locus_tag="VGR03_17080"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996049.1"
                     /translation="MRIAAAGKCFIPGIIVATILCSSTNAQQFDQKLYSEMRWRCIGP
                     FRGGRTVAISGVPHQPNVFYMAAVNGGVWKTTDFGNTWNPIFDDQPTGSIGALAVAPS
                     DPNFIYAGSGEGLQRPDLATGDGVYKSTDAGKSWTHLGLRDAQQITAILIDPKDSSRV
                     FVAAEGHPYGPNAERGVFRSSDGGQSFQKVLYKDENTGAADLAFDPANPQTIYAVLWA
                     ARVAPWEVRSGESFISAGSGIYKSTDGGSNWRPLTKGLPAAADGLGRIGIAVSNSEPN
                     RLYASVEAKKNAGVYRSDDSGETWKQVNADHRIGGRGPGAMGIAVAPDNPEIIYVANT
                     TTWKSSDGGKTFVGFKGAPGGDDYQRIWISTENPQIIALSSDQGAIISVNAGATWSTW
                     YNQPTAQFYHVTTDNRFPYWVYGSQQESGSVATQSRSDYGEITFRDWHLIGIFEYGYI
                     AVDPLDPNILYGDWLTRTKQDIGEYAKVTPEPIRRGEYRYTRTLPVVFSPLEPHTLYF
                     AANVLFKTTDAGNSWQVISPDLTRESYDIPASLGVFAASDPEKGKHRGVIYAVAPSFK
                     EANTIWAGTDDGLIHITRDGGKSWQNVTPPQLKPWSKVSVIEASHFDAGTAYAAINSF
                     RLDDLRAHIYRTRDFGKTWAEITKNIPDGGASNVVREDPVHKGLLFAGTEGSVYVSFN
                     DGDDWQPLQLNLPNTSMRDLAIHGDDLIVATHGRSFWILDDVTPLRQMSADIAKEGVH
                     LFAPQEAIRFRWNRNPDTPLPPEVPAGKNPPDGAIIDYYLAVAAKGAVKLEIFDEQDH
                     LVRRYMSSDKLEPLEKTAGEHPIPMYWVRPAQILSGEAGMHRFVWDLHYAPPDSLGHE
                     FPISAIVHDTPKYPLGTWALAGNYTVKLTADGKSYSQPLIVKMDPRIKTPLADLRKQF
                     EMQSGSVEGMNESFEALSQVQSVRAQLKERVAKAGKGALAEAIAALDKQAAELEGAAQ
                     SNFFGLPPGAKQPENFSSLNQHFGSILAVADSADAAPTTQATAVYEELEGAIEKLVTR
                     WTKIRQQDIPALNAELKKARLAAVDPNKLPDAAPSADADGDDEP"
     gene            15142..15420
                     /locus_tag="VGR03_17085"
     CDS             15142..15420
                     /locus_tag="VGR03_17085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEV1996050.1"
                     /translation="MEEIAISKFKATCLAVLERVRRTGEPIVVTRLGQPIAKITPPGN
                     GERIPRLGGGRGTGVILGDIVGPISEESDWEAAQDPDLIEAKKSDKTK"
     gene            15417..15812
                     /locus_tag="VGR03_17090"
     CDS             15417..15812
                     /locus_tag="VGR03_17090"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEV1996051.1"
                     /translation="MKLLLDTHIWIWSYLEPHRVSSEVVRELSSPANEWFLSSASVWE
                     MVMLLEKKRIQLDGDFGVWFKNSTEELKLTEIPITWEVAHELRFTTLSHRDPGDRFLV
                     ATAKVYDLTLVTADERLMQIPGLKVLANR"
     gene            15900..>16804
                     /locus_tag="VGR03_17095"
     CDS             15900..>16804
                     /locus_tag="VGR03_17095"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996052.1"
                     /translation="MRTAFFSRFAKIAPFFTAATIFAASIASAQQVPEQLLSDMKWRM
                     IGPFRAGKVNAVAGVPGNPGVYYFGANGGGVWKTTDGGTVWKPIFDEEPVASIGALAL
                     APSNPNIIFVGSGENNLYADITYGNGVYKSNDAGATWQHLGLAETRHIARILIDPRNP
                     DIVLVAAMGHSFGSNEERGVFRSADGGRSWKKVLYKDNVTGAVDLCYEPGNPRVVYAT
                     LWHGIRKPGQKGTSFGAGGGLYKSTDEGITWTQIAGHGLPAGDWGRSGVAVAPGNHGQ
                     RVYLILEAKEKDGGLYRSDDAGASW"
BASE COUNT         3700 a         5429 c         4252 g         3423 t
ORIGIN      
        1 ccccgcatca ttcgtcttcc aaatgcctcc gcccacgctt ccgaaataga aaacctccgg
       61 ctgtccgcgc acgcccgcca ccgccagcgc gcgcccccca cgagctggcc cgatcaatcg
      121 ccaccgcagc tcctggaata gcttcgggtc gaactgtttt ggcgcgtccg gtccagcaaa
      181 cagtcgaacc gacgtacaaa gcacgaatag aacgaataga aatgcgagag cgaagcggcg
      241 cgatatcacg tctggagtct cctggcggaa agcaagcttc tccaaagatt gctgagtata
      301 tctcagccgc ataacaaaac cgcccggctc tttcgaaccg ggcggcaggg aatcatttgg
      361 ggggagaatc gtctaggctg tttccaactc ctcgatctct tccatcacca ccggcttcgt
      421 ctgacgaatc ttcaacttgc ccgtgacgaa gtagatcaag aagccggaca tgataatgag
      481 cacaccgaac aggaggccgt tcatgcccag cacggtttcg tggttgcttt ccaccatcgc
      541 caggctcagg agcagcagcg ggaatattcc gcaagtaatc gcaccggcca gcccgcccgg
      601 gacgcggaat tggcgcttca acttcggctc gcggatgcgc agcgccacca gcgttacgaa
      661 ctccagcatc aggctgatcc cgtagagcat gatgtccagc gttacgagcc gtttgaaccc
      721 cagccccagg cacaacgccc agcacgtcgc gcagaaaata atggccaccc agggcgcctg
      781 agtccgcgcg ctcatcttgc caaaaaactt cggcagcatg ccgtcccgcg ccatggccaa
      841 tgggagacgc gaatagctca gcaccagcgc gttgaacatg ccgaacgcgc tcatcatccc
      901 gccgaggacg atcaggaacc tgagccattc gaatcccatg aacttcccgc caacgagacc
      961 cgccacttcg gcccaagcgc catcactccc gaacgtcgag gccgggatac ccgcgaaata
     1021 caccgccacg aacgggagca cgtacgtcag cgccaccagc accactgctg caatcatggc
     1081 cttcggataa gttctctgcg gccgctcgac ctcctgtgca atcgtcgagg cgttatccca
     1141 gcccatgtaa ttccacatcg ccaccagcac gccgccaacc aagccgagcc ctgttgcggc
     1201 cggcgcggca tggccttccg caagcgcgcc cattttgagt ggagccatca ccacgatcaa
     1261 cgcgaacggt gccgacagca ggaagaaaag ccacagcgac gtgattccta cgacgcgaat
     1321 tccagcaaga ttcagcaccg cgcaggtcac caccacgaaa agcccagcgt agattccatg
     1381 attcccaacg ccaaaccacg gggacatctg cttcaagtag aagacaaaca gcgtcggata
     1441 gatcgccatg tcaaagatgc tcgcggccaa cgagagccac gcttcctgaa agccccagaa
     1501 attccccaac ccgcggcgca cccacgcgta gtagccaccc tcctgcggca gcgcgctcga
     1561 tagctcgcca atcataaacg ctgtcggcag gcaccacagc accggcagaa acaacaagat
     1621 caagatcccg tgtccgtagc ccgcgccgtg aatgatctct tccgtcccat acgttccccc
     1681 agacaccatg aagaacgtgg cagccacaag cggccagagc gtaagcttag cgactctttt
     1741 tcttgctgtt gagattgcgc gggaaggtag ctgggtggga agggataaag aactcaattt
     1801 ttactctcgt cctcctaaac gaaactcgtt cgcgccgctg cggtaaaacg agttcgcatt
     1861 cgcagaatac aagagtcgcc actgcatgca agaagaaaat gcgcctgagc gaatattttt
     1921 tctctctcgc taaccgtact tccgtactac ctgagcgccc gccctgcgaa cccctcgccg
     1981 ccgcttcccg attctctcct cgcgccgtct atcagagagc ctgtgttaca cttcgccgtt
     2041 tgttttattg ctctcataac cgttcaaggg gtcattgcga atgaagttct cttttcgttg
     2101 ttcagctctt tgcgcagttc tggtactcgc gccgctcgct ctcgctcagc aacccgctcc
     2161 tcgcgccatc accattgacg actatttcca gattcgcgag gttcgcgacc cccaactgag
     2221 ccccgatgcg aagtgggtca cttacaccgt caagacggcc ctcctcaaag aagacaaaaa
     2281 cgaagagcgc atctggatgg ttccggccgc tggcggtgca cccctcgcat tgaccgccga
     2341 aggcgtttcc tcctcccacg ctcggtggag ccccgatggc aagttcatcg ccttcctttc
     2401 cgctcgcggt gaagacaagt cccaggtctg gctgctcaac cggcacggtg gggaagccga
     2461 gcgcctcacg gacacgcctc aggacgtcga ggactttgcc tggtcacccg acagccgcca
     2521 gctcgttatg attcttcgcg acgccagccc cgaggaactc gaagccgcca aggaaaacaa
     2581 gaacaagggc gtcgcacacg ctgcgacctc cggtggaagc gacgccaagg acaagaagaa
     2641 agcgcaaaaa ccctgggtca ttgaccgttt gcaattcaag ctcgacgaag tgggctacct
     2701 tgatcgccgc cgtacgcacc tctacgtgtt cgacttagcg gcgaaagccc tcacgcagat
     2761 cacctccggt gattacgacg actccgagcc tgcctggtct cccgatggca agcggctagc
     2821 cttcagcagc aatcgctcga cgccggaccc cgacgccgca tccgaccaga acatctgggt
     2881 cgtcgccgcg aacaaccccg acattggtgc gcatctcact caagtcacca cgaatcccgg
     2941 cgcggatggc tcccccgctt ggtcccctga cggaaagtgg atcgcttacg tcacgcagct
     3001 cgatccccac ctctttgatt acgccaccaa gcatcttgcc gttgctcccg ccaccggtgg
     3061 cgaagcccag gtcctaaccc ttgctctcga tcgcatggtg atcgtccccc gcttttcccc
     3121 cgacgggaag ttcatctatt tcatcgccga cgatgacggc acccaaaacc tctgccgcat
     3181 ccccgtcacc ggcggagaga tcacccgccc cgtcgctggc cgcctgatgg tcaacgacta
     3241 ttcgctcgcc aagaccggag acctcgtcgc ccaagtcgcc actctcgacc gccccgacga
     3301 gctctacacc attcccgacg gcaaactgac ccagatcacg cacaccaacg acgctctaat
     3361 aagtcagctc aagatctccc aaagcgagta cgttcacttc aagagcaagg acggcacgat
     3421 cgtctctggc tatctctaca agcccctcga ctacgtcccc ggcaagaagt accccaccat
     3481 ccttcgtccg cacggcggcc cagtctgggc ctattacgcc gaattcgatc atctaccgca
     3541 gctctatgcg gccaacggct acgccgtcct ctttccgaat cctcgcggct caaccggcta
     3601 cggccaggac ttcgccaaag cgatctgggc cgattggggc aacaaggatt ttcaggacga
     3661 tatggctatg gtggattacg ccatcgagct gggcatcacc gacccggaaa agctcgctgt
     3721 tggcggctgg tcgtacggcg gcatttccac ggacttcatc atcgcgcaga ccaaccgctt
     3781 caaagccgcc atctccggcg ccggaagcgc cctcttcatc agctcttacg gccacgacca
     3841 ttaccaaaag gactatgaaa ccgagttggg ccacccctgg gaaaacaaag ctgtctggga
     3901 aagagtctcg cccttctatc gggtcgcgaa cattaccacc ccaacgctct tcatgggcgg
     3961 cgagattgac tggaacgtcc ccatcctcgg cggtgaacaa atgttccagg ctctgaagcg
     4021 cctcggccgc accaccgagc tagtcgtcta ccccggcgaa taccacgaat tcaagacgcc
     4081 ctcccacatc aaggatcgcc tcgagcgcta cctcgcctgg tacgcccact atgtcaaagg
     4141 cgattccgcc cccgcgcgtc cccccgagcc cccgccagct acgccccctg ccaaacctgc
     4201 caactagggc cgtttctcct ttgtaggggc gcagcaccgc tgcgccctct cttctctgtg
     4261 tcctctgcgc tctccgtgtt ctctgtgtaa aactcttcgg tttttttcct tccttgcctt
     4321 gcccgaattg ctctcctcgc gttaagctcc atcccgttcg cggaggcatc gcatgaggaa
     4381 tcgcttctcg gttgtcgcag cactactcct aactcctttt cttgctcact cccaaacccg
     4441 cttcaccgtc ggcaccgcct ccgccgcccc cggcgaaaaa tccactggta ctctcgaagt
     4501 ctccgcaggc gttgacgccg ccaccagcat ccccgtcgtc gtcgtgaacg gcgccaagcc
     4561 cggcagagtt ctcgcgctcg tctccggcgc ccacggcacc gaatacgtct ccatcatcgc
     4621 catcgaaaaa ctcatcgccg ctctcgaccc cgcgcaagtc accggcaccg tcattctcgt
     4681 cccgctcatc aacatccagt ccttcgagca aaaagtcccg cacgtcaatc ccatcgacaa
     4741 caagagcatg aaccgtttct accccggcaa agccgacggc acgcaaaccg accgcgcctc
     4801 ctatctcatc accaaggaaa tcgtcgaccg ctgcgactac ctcatcgact accacggcgg
     4861 cgacctcgac gaaagcctcc gcccctacgc ctactgggcc cccaccggcc gcgaagccca
     4921 ggaccgcacc tccaaggaaa tggtcctggc cttcggcctc gatcacatca ttatctggcg
     4981 cgaacgtccc accgatcccg ccgtcacccg ctacctcgat aacaccgcct ccgtccgcgg
     5041 caaaccctcc atcgtcgtcg aagctggcca cgccggcacc gtcgagtccg acgacctcgc
     5101 cctcctcgtc aacggcaccc tcagcactat gcgcgccttg aagatgctcc ccggcgatcc
     5161 gcatcccatc gaaaatcccg tctggattga taagctcgtt gacgtcctag ccgacggccc
     5221 cggcatctgg tatcccctcg tcaagcgcgg cacctacgtt tccgccggca tgaaactcgg
     5281 caccatcacc gactatgtcg gcaaagtcat cgccgagccc cgcgcccccg tctccgccgt
     5341 cgtcctccac ctcaacgccc tcccctccct caaaaaaggc gacaacatcg ccgacctcgg
     5401 cgtagtagcc ccccacgccc cctgagcctc catattttgt agaggcgact ttgcgtcgcc
     5461 atcttctctt gcagcgtcga acttcgcggt tccttgcgtc ccggttctga ccggtaacct
     5521 cgccacgcaa ccctgccctt tgcctcattt ctgtgataca cacggaatcg tccgaaggag
     5581 gtcccatgcg caaatggggc atcgtgattt ccgtcttcta cgctctgatc ctcctaggcc
     5641 ttatcgtgcc cggaagcatg tttatcgccg gaggtgattt ctcgaagtgg tcgggcctcc
     5701 tccctggcat ggagggcact tacaccgact tgacgttttg gattctagcc ggagccatcc
     5761 tggccagtca ggcccttctg cttttcctgt ctgtcgacac gtctcacaaa cggctcaagc
     5821 ctcgcgccca cattctcgtt tcctgcgccg tcgtcgcggt cctcaccgca ctcctcacct
     5881 ccgctgtaat ctggtcccta ggcgtcgccg tacgcggcga caaattcttc catttcttct
     5941 tcgatttcgc ccacggtttt tttgatgagg ataatcatct cctgatcttt tggggcggcc
     6001 tctggttact ctggggaatc ctcttttatt tctatttccg gaactcctca gcggttgtaa
     6061 cgaagatcat ttcgtggctg ctcaagggca gcgtcctgga actcctgatc gttgtcccct
     6121 gccacatcat cgtccgccgc cgtcacgatt gttccgcgcc gctcgccacc agcttcggcc
     6181 tcgccaccgg cctcgccatc atgctcctct cctttggccc cagcgtcctc ttcctctaca
     6241 gaaaacgcct cgactcctac ctgcctcgtt cttccgtgta gcggcgactt catgtcgcca
     6301 ccttccttct tgaatcttgc agcggccgct ttctcgtccc ccatgtagag gccgcccttg
     6361 aggacggctc ttcaatctcg cagatttccg caccgccgcc gctccttttg cacagaagag
     6421 tttttcgatt ctccttgaca cccacattct tggggaacaa gctcgtgtcg aggtttcgcc
     6481 gtttaaggcg agactcccgc tccatagtcc gagcgcagtt tctctaacag ttaagctccg
     6541 catcgagcgc caagagggca gtacgtcctc ggctcctttt tctccactcg cgcggggcct
     6601 aacctcctgt ccgacgtaca gaactgcacg ccccagaccc cagcggttcc agaccacaac
     6661 actcgagtcc gactcgcttc atgcgaggaa agtttttgct tttctcaaaa aggagaacat
     6721 cccatgtcag aaaataacaa agctatcgtc cgtcgtctga ttgaagaact ttggaacaag
     6781 ggcaatctgt cgctggcaga ccagcttttt gctcccaact acgaacacca tgacgcttcg
     6841 tcacccgatt ttgggcgcgg cccggagagt gaaaagaaga gagcgactct ctaccgcact
     6901 gctttccccg acattcgatt gacgatcgag gacatcattg ccgaaggcga aacagtaatg
     6961 acccgttggt cctgccgagg tacccataaa ggggatctca gcgggattgc gccaaccggg
     7021 aaggagttca acatcaccgg cgtgaccatc gcccgcctct cgaacggcaa gctggcggaa
     7081 ggctacgtca actgggacgc tctgggcctt atgcagcagc tcggcgtcgt ccctgagctc
     7141 gccaaagcca aagccgccgc cgccccaagg taaccattct cactttccgc aagaaaccgg
     7201 aaggcccgac cacatatcgg gccttcccac aactcttcgt gcgccctcac cccacaccgt
     7261 catttcgaac ggagcagggc gattttttct tccccttcgc tcctgcgaag ggtcgccctg
     7321 cggatgtgag aaatctctct tcgtctttgc tccagattcg catttgaagc ccccgtctct
     7381 cagtgtcaca atccacgccc atggagcgca ccttccatgt ctacctcatg gccagcaaat
     7441 caggcgtcct ctacctcggc gtgaccggca acctctctca ccgcgtcggc cagcacaaag
     7501 aaaaatccgt ctccggcttc acgcagaaat acaacatcac caagctcgtc tggttcgagc
     7561 cccacaccag catccgctcg gctatctccc acgaaaagga aatcaaaaag tggcggcgct
     7621 cgaagaaaat cgcattgatc gagtcgctga accccgaatg gaacgacctc agcctgaacc
     7681 tctgagtcct tcctcactcc gacctgcgcg ccaccacccc gcccgcactc cccaaccgcc
     7741 cacgccacac cgtcatttcg aacgaagcag gccgactctt tcttccgcat tcgctcccgc
     7801 gaatgcgtcg gcctgcgcag agagaaatct ctcttctctt tctgcaccgg ccgccgccgc
     7861 cccgccacca aagactggcc cgattcctac gtctgcgcct tcgcctccgc cccccagctc
     7921 accctcgcat cttcgaccgc cccctcagcc gccgcgcgac aaattcccac ctgctttctc
     7981 cctgatctcc ccgacgttcc gatgttcaga cgttcggaca tttagacgtt cttagaccat
     8041 ccaccttccc ccgctctgcg gcaaaacgcc ttttcgcctc gcggcaagcg agccctgaaa
     8101 aaagccccgc agcgtcttac cttgctatct gggcgcacac aactaataga cgctctctcc
     8161 acaacaaccc aatatctggt gtttgtcatt gaatcttgtt tgtctaacgt gatactatgc
     8221 gtccactgag gtcggacaaa tggaaaacga aagcaacgcc ggtcttcggc acctcctaga
     8281 cttggcaagg aatgaaaaca acaggcagcg ccctgaccta gcgttgggct accttcaaga
     8341 ggcgagatcg gaaattgaac ggcgcgaagg tgaggcaatc tgggcagagc accatctgct
     8401 cctcgcagaa gcttacgcgg cgaagtgcga ccctgcggcc gagccgcttt ttgaagagct
     8461 ttttgacaga ctacggaagc tacccgaacg ccacccagag ctcgaactcc tcgcgcacga
     8521 gcatttcgcg gacttcctcg tccgcctcga tggtcgccgg tcagtcgcga gagaccattt
     8581 cgagcaagcg aggaagctcg cgatcgaaga aaatctccgg gaagagagcg ctcggacaga
     8641 attgaaattg accaagctcc acttggagat ggatagcgac ccggagcttc ctaactttaa
     8701 atccttccgg cgtgtagcga aaaatggggg ctatacttac caagtccaat tggcctcatg
     8761 gcagcgccat tacctaggcc agcaggggct taacaaagga ctgcgttatg cccgcaagcg
     8821 caatgtcgcc agcgatggat atttcgcaga catcttggaa tcggtgaaga agtccacgga
     8881 atgaagtcca agaccaaccc gtacatggtc agaatctctt ccctagttgg ggaagcgaat
     8941 cagaatctcg ccgtaaggga tttcgttcaa tctcacagac agcaaggtga atccctggag
     9001 tccgtagcaa cgaggctcgg catcgccgaa atcaaatacg aaccacttcc cttcgatggt
     9061 ggaattttcg aggacggcgc tacaagagtg ataaaaataa attcgttcag accagaggtc
     9121 cgcagaaact tcaccttggc ccacgagttg gcacatctaa taatggctgc ctcgttcgaa
     9181 gagaagaaaa ggaagccctg cccggacgac ccgaacctgg agcgcacgtg tgactccatc
     9241 gctgccgaac tcctcatgcc ttacgaagaa acagtcaggc tcgctactca attggggcgc
     9301 cagtccccgg aaaagcttgg caccctcgca aaaaaatttg gcgtgtcact cgaaactgcc
     9361 gcgcgccggc tccacacgga attgggactg tggaaactgc cgatgggatt gtgggagtgc
     9421 gggccagtcg ctagagagct ctggttcgta ggcaaacgcc cctggagatc gccgcgacag
     9481 tctttctccg cctttgagtt ggctcgagaa tcgcgtattc ctatcgtcac gcgggaacgc
     9541 tacccaaggg ggactcatac agaactggtt gacctcaaag tcctacatat cggaaataat
     9601 aaaatcctcg gtgtcatcgc tatctagcaa gctcagcgat tcaggggccg gtggccgaca
     9661 cgcgcgccct ggttgtctcg gctatggcct gcgtagggaa atagttcgtg ggtccctaag
     9721 tagtcgcggg aagcggaatt tccctccacg gttggggcgc gccgcagcgt tcctgcccgc
     9781 ttgactttca tgctatgagt atagaataaa gtgaccgtgg aagataaatc cccagtttcc
     9841 aaaattttct cccaacttgg cattagtaaa tccatgcaac gaatcaagtt tggcggggtc
     9901 gtgggcaaac agactctcct aggcgtaatt gggtcggcga cgctagtttc gatcgcttgg
     9961 ggagccgatg cgacaactaa attagtgatc gcagttttgg caggtgtcct tcttatcttg
    10021 gttgctttat tgaacttttc cttcgccaac aagcacgccg ccgaagctat gcttgaagga
    10081 accgagatgc ttgcttttca gcatcaagtt catgctgcca aatcagcaga agtgcctcaa
    10141 aacgcccccg ttataccgaa tcccggcggg tctgcacccc aactcaatcc gtcacaagag
    10201 actgagcaat gaagacgaag aggtttgtcc acgttggatt taattttgaa gacaccacgc
    10261 ccccaattaa gcaattggag gcgaccttcg ataaggcaaa ggactggatt cggtacggta
    10321 atcagtgctg gattctctat acagctctag acttagatga atggagagac agaatccgta
    10381 gaactaaagg actgaaagat actgtcagct tccttgtggt tgaatttgac taccattctg
    10441 gctatatgtc agattttgag tggaagtggt tgcaaaagga ccgttcataa ccgaaaccgc
    10501 gggtagcagg cccctagccg cgggtacgaa gggcgtgccg cacccttcac gactttggtg
    10561 aagggtgcgg gtgttgaatt tgactctcgt ctggtatcca actagtccgc ggtaacccct
    10621 tccacttcaa tctcttccta cacggcaacg ccaaatactt aactttttac agtaccacac
    10681 cataatgtac ttgactttta gctattctat gctataatag aatatggccg tttcgctcca
    10741 gccccgccga acccgtaagt cccgctccat ctttgtcttg ccccttccgc ccctcctgtt
    10801 cgacgatcga ctttcaatcg aagatcccga ccgggtcggg acgttcaaac attccccgtt
    10861 cctctcctgc cccattccca ttttgaaact caccctccct catctcagca ggaatctccc
    10921 ctttcttttc aagtgcttac gggagcccat tttgcaaccc ctttgttttc aagtttatgc
    10981 aggaatgggg ggggtgtaca cgcctccacc aaaaaaaagg aacaactcat gaacaccaca
    11041 gtcgctagcc caaacttcac ccccgatccc gtcgctgtcc cgccgaaatc cacttcgccc
    11101 cgctgtcctt atctttatcc caacggcaag cgctgcagcc tccccggctc gcaagttcac
    11161 tctggctctt gcctccgcca tgggcagcca aacgctccca ctgctattcc tgtgcccgtc
    11221 caaaacgact cggaagacct ctcccccgaa cttctccccg agctctccga gttcgagtcc
    11281 gccattgaca tcaacaagtt cctcgcaagg ctcctggtcc ttgttaccaa aggccgcatc
    11341 tccccgcgcc gtgcctccgt cctgggttac atcaccaacc agctcctcca ctctcaccgc
    11401 gccatcgacc gcgaaaactt tctccaactc gaggacgccg gccctccccg cgtcgagatc
    11461 ggcgacatcc cgcgcccagc ccgttcgcag cccaatcgta acgccggccc caccatcatc
    11521 tgggatgtcc ccggcgtaag tatcgaccgc gagaaaacac cctcatgacc atttatcacg
    11581 agtgtcgaga gggaagcgcc accgatttcg tggttctcgt ggcacagcca ttcctgcttg
    11641 ccctgagctt gccgaaccgg gctgtgctct tccacgaatc accagtcaca agtcaccagt
    11701 cacgtctttt ccattcgcgt gtctgccagt cgccacgcat tgctccgctc ccgcagccag
    11761 agtatcctct cccttctcgt ggaggactcg gatgcgaatc gctgcggcag ggaagtgctt
    11821 catccccgga attatcgtcg caacaattct ttgcagttca acgaacgcgc agcagttcga
    11881 tcaaaaactc tattccgaaa tgcgctggcg ctgcatcggc ccgttccgcg gaggccgcac
    11941 cgtggccatc tccggcgtgc cccaccagcc gaacgttttc tacatggccg ccgtcaacgg
    12001 cggcgtctgg aagaccaccg atttcggcaa cacttggaat ccgatctttg acgaccagcc
    12061 caccggctcc atcggcgcgc tcgccgtggc tccttccgat ccgaacttca tctacgccgg
    12121 cagtggcgaa ggattgcaac gccccgatct cgcgacgggc gacggcgtct acaaatccac
    12181 cgacgccgga aaatcctgga cgcacctcgg cctccgcgac gcccagcaaa tcaccgcgat
    12241 cctcatcgac ccgaaagatt ccagccgcgt tttcgtcgcc gccgaaggac atccctacgg
    12301 tcccaacgcc gaacgaggcg tcttccgttc cagcgacggt ggccaatcct tccaaaaagt
    12361 tctctacaag gacgaaaaca ccggcgccgc cgatctcgct ttcgatcccg caaatccgca
    12421 aacgatttac gccgtgctgt gggccgcgcg cgttgcgcca tgggaagttc gcagcggcga
    12481 atccttcatc agcgccggca gcggcattta caagtccacc gatggcggaa gcaactggcg
    12541 tccgctcacc aaaggtcttc ctgcagcagc cgatggcctg ggccgcatcg gcattgccgt
    12601 atcgaacagc gagcccaacc gcctctatgc cagcgtggaa gcaaagaaaa atgccggtgt
    12661 ctatcgctct gacgactcag gagaaacatg gaaacaggta aacgccgacc accgcatcgg
    12721 cggccgcggc ccgggtgcga tgggcatcgc cgtcgcgccg gacaatccgg aaatcatcta
    12781 cgtcgcaaac accacgacgt ggaagtcatc ggacggtgga aaaacatttg tggggttcaa
    12841 aggcgcgcct ggtggcgacg attaccaacg catttggatc agcacggaaa atccgcagat
    12901 catcgcgctc agctccgatc agggcgcgat catcagcgtg aacgctggcg cgacgtggag
    12961 cacttggtac aatcagccaa cggcccagtt ctaccacgtc actaccgaca atcgttttcc
    13021 gtattgggtt tacggctcgc agcaggaaag cggctccgtt gcgacgcaga gccgcagcga
    13081 ttatggcgaa atcacttttc gcgattggca tctcatcggt attttcgagt acggatacat
    13141 tgccgttgac ccgctcgatc cgaacattct ttatggcgac tggctcacgc gaacgaagca
    13201 agatatcggg gagtacgcga aggttacgcc cgagccgatt cgacgcggcg aatatcgcta
    13261 tacgcgcacg ctgcccgttg ttttttcgcc gctcgaaccg cacacgcttt atttcgcggc
    13321 caacgtgctt ttcaaaacga cggatgcggg aaacagttgg caagtcatca gtccggatct
    13381 cacgcgcgaa tcttatgata ttcccgcgag tctcggtgtt ttcgcggcga gcgatccgga
    13441 aaaaggaaag caccgcggcg ttatttacgc cgtcgctcct tcgttcaagg aagcaaacac
    13501 gatctgggcc ggcaccgatg acggactgat tcacatcacg cgcgacggcg gcaaatcctg
    13561 gcaaaacgtc acgccgccgc agcttaagcc gtggagcaaa gtttccgtca tcgaagcttc
    13621 gcacttcgat gcgggcacgg cgtacgcggc gatcaattct ttccggctcg acgatctgcg
    13681 cgcgcacatt taccgcacgc gcgatttcgg caaaacgtgg gcggaaatca caaaaaatat
    13741 ccccgatggc ggcgccagca acgtggtgcg cgaagatcct gtgcacaagg gcttgctctt
    13801 cgctggcacg gaaggctccg tctacgtttc cttcaacgat ggagacgatt ggcagccgct
    13861 gcagttgaat ttgccgaaca cctcgatgcg cgatctggcg attcacggcg acgatttaat
    13921 cgtcgccacg cacggccgct ctttctggat tctcgacgac gtcacaccgc tgcggcaaat
    13981 gagcgcggac atcgcgaaag aaggtgtgca tctcttcgca cctcaggaag cgattcgttt
    14041 tcgctggaac cgcaaccccg acacgccgct gcctccagaa gttcccgccg ggaaaaatcc
    14101 tcccgacggc gccatcatcg attactacct ggcggtcgct gcaaaaggtg cggtcaaact
    14161 cgaaatcttt gacgaacaag atcatctcgt gcgtcgctac atgagttcag acaagctcga
    14221 gcctctggaa aaaaccgctg gcgagcatcc catccccatg tactgggtgc gcccggcaca
    14281 aattctctcg ggggaagcag ggatgcaccg ttttgtctgg gacttgcatt acgccccgcc
    14341 ggattctctg ggacacgaat ttcccatttc cgccatcgtg catgacactc ctaaatatcc
    14401 gctgggaaca tgggcgctag ccggcaacta cacagtgaag ctgactgctg atgggaagag
    14461 ctattctcaa ccgttaatcg tgaagatgga cccgcgcatc aagactccgc ttgcggatct
    14521 tcgcaaacag tttgaaatgc agagcggctc ggtcgaaggc atgaatgaaa gctttgaagc
    14581 gctctcgcag gttcaatcgg tgcgcgcgca attgaaggag cgcgtcgcga aagcagggaa
    14641 gggcgctctt gccgaagcga tcgcggctct cgacaaacaa gctgccgaat tggaaggtgc
    14701 ggcgcagagc aatttcttcg gcttgccgcc cggggcgaag caaccggaga atttttccag
    14761 tctgaaccag catttcggca gcatcctggc ggttgcggac agcgccgatg ctgcccccac
    14821 gacgcaagcc acggccgtct acgaggaact tgaaggggct atcgagaagc tcgtgacccg
    14881 ctggacgaaa atccggcagc aggatattcc ggcactcaac gccgaactca agaaagccag
    14941 actcgctgct gtcgatccga acaaattacc ggatgccgct ccgtccgccg atgcggatgg
    15001 agacgacgag ccataaacca taaaaaggat aatgagtggg aaagtgagaa tgacgggaaa
    15061 atgaccgtag tcagaccatg gtcggactat gattggacta tagtcagaat ctgtgctatt
    15121 gtggcgatgg agagccatgc catggaagaa attgccatct ctaaatttaa agctacttgt
    15181 cttgcggtcc tcgagcgtgt gcgcaggaca ggcgagccga ttgtggtgac gcggctcgga
    15241 cagcctatcg ccaaaatcac tccccctgga aacggggaac gtatcccgcg actgggtgga
    15301 ggccggggga ccggcgttat tctcggcgat atcgttgggc ccatcagcga ggaatcagac
    15361 tgggaagcag cccaagatcc agatctgatc gaagcgaaaa aatcagacaa gaccaaatga
    15421 aactgctcct ggacactcac atctggattt ggagctacct tgagccgcat cgagtcagtt
    15481 ctgaagttgt gcgtgagctg tcatcgccgg cgaacgagtg gtttctctct tcagcaagtg
    15541 tctgggaaat ggtgatgcta ctcgagaaaa aacggattca gttggatggt gattttggag
    15601 tgtggttcaa gaactcgacc gaagaattaa agctgaccga aattcctatc acctgggaag
    15661 tggctcacga attacgcttc acaacactta gtcatcgcga ccccggagac cggtttttgg
    15721 tggcgacggc gaaggtttat gaccttactc tggtgaccgc agacgagcgg ctgatgcaga
    15781 ttccgggatt gaaggtgctc gctaaccggt gaggaaactc cacctcattc gagtaaggtg
    15841 aattcgtttt gcggagatgt cgcaggcaag ctatcctcac gcgcgaggga gacccattaa
    15901 tgagaactgc ctttttttcc cgattcgcca agattgcacc gttttttacc gctgccacta
    15961 tttttgctgc cagcatcgct tcagcgcaac aagttccaga acagcttctc tcggacatga
    16021 aatggcggat gatcgggcca tttcgcgcgg gaaaggtgaa tgccgtggcc ggtgtgccgg
    16081 gaaatcccgg cgtctactat ttcggggcga atggcggagg cgtatggaag acgaccgacg
    16141 gcggcacagt gtggaagccc attttcgacg aagaaccggt cgcgtccatt ggggcgttgg
    16201 cgcttgcgcc ttcgaatccg aacatcattt tcgtgggaag cggagaaaac aatctctatg
    16261 ccgacatcac gtatgggaat ggcgtctaca aatcgaatga tgccggagca acctggcaac
    16321 acctcggact tgcggagaca cggcacatcg cacgtattct gatcgatccg cgcaatccgg
    16381 acatcgtgct ggtggcggcg atggggcaca gcttcggttc gaacgaggag cgcggcgttt
    16441 tccgttccgc cgacggtgga cgcagctgga aaaaggtttt gtacaaggac aatgttacag
    16501 gggcagtgga cctctgttac gaaccgggga atccgcgcgt ggtttacgcg acgctgtggc
    16561 atgggattcg aaagccaggg cagaagggaa cttcgtttgg cgcgggcggc gggctctata
    16621 aatcaaccga tgaaggcatc acttggacgc aaattgccgg gcacggtctt ccggcgggag
    16681 attggggccg gtccggcgtt gcggttgcgc cgggaaatca cgggcaacgc gtttacctga
    16741 ttctagaagc gaaggaaaaa gacggcgggc tctatcgttc cgacgatgcg ggagcaagct
    16801 ggaa
//
LOCUS       DASYVO010000492         5026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_82671, whole genome shotgun sequence.
ACCESSION   DASYVO010000492 DASYVO010000000
VERSION     DASYVO010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82671"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            568..1980
                     /gene="fumC"
                     /locus_tag="VGR03_17100"
     CDS             568..1980
                     /gene="fumC"
                     /locus_tag="VGR03_17100"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEV1996053.1"
                     /translation="MSEARQTAKTPTRTETDSMGSVQVPADLYYGAQTARSLVHFAIG
                     KDTMPPELIPAFGILKKACALVNQELGKLSAEKARLISQAADEVIAGKLNDHFPLRVW
                     QTGSGTQTNMNVNEVISNRAIELAGGVLGSKKPIHPNDDVNMSQSSNDTFPAAMSIAA
                     ATETSRRLLPAVKKLGDALNKKAREFAEIVKIGRTHLQDATPLTLGQEFSGWVSLLDR
                     DVKRIEQALDGLYDLAIGGTAVGTGLNAPPEFGERAARKIAELSGLPFRSHPNKFAAL
                     SAHDEIVFASGALKTLAASLMKIANDVRWLASGPRCGLGELSLPENEPGSSIMPGKVN
                     PTQCEAVTMVAVQVMGNDAAIGFAGSQGNFELNVFKPVMIFNYLHSVELLSDACASFV
                     DHCVVGIEVNRKQIDFYVKNSLMLVTALSPKIGYDKAAKAAHTAHHENLSLREAVIKL
                     GYLSGEEFDSLVRPADMTHP"
     gene            1977..3077
                     /locus_tag="VGR03_17105"
     CDS             1977..3077
                     /locus_tag="VGR03_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996054.1"
                     /translation="MNVSFTQITNAVLFVAGSLVPTFASAQAYSGPKCVGPVCIDRKL
                     PFERLAVQLGGPSSGGGTYGYRTKSGQAFLIVTEDRWGQVGSIHLRDFAKWRTWRQRD
                     QKLTTEDLRGWKTPQGIGLGSLEEEVLRAYGKPSGVANLELEHSQREMGKRMLIYKGR
                     FKEIFPAARFRIRDGKVSWIDLENDAFLGPDCLGPYCTYGELSLNSLLRKLGLPPQKN
                     TASPLECFQSQDARASLHFRTDVESPSDLDDVLLSDFPNCLHMRKRIATNGLRAWKTP
                     EGIGLGSSEEDVLKAYGTPSAEKELDAQNHGFAMKGLIAGYRAGDNQTRVGNKVLIYG
                     PRELQVVEFGIRDGKVSYIWLKDSDFWLKDGE"
     gene            3107..4150
                     /locus_tag="VGR03_17110"
     CDS             3107..4150
                     /locus_tag="VGR03_17110"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEV1996055.1"
                     /translation="MIRRTVFLSFCFLPYLHPLCAQQVNAPAAYTLKATPKTVAWGYY
                     DASTPPVLRIKSGDTVEIQTLITTSPKRLEDAGLPPEQVEQSLRDIHAQVKDKGPGGH
                     ILAGPIFIEGAEPGDVLEVRILTARLAIPYAYNAFGPGRGFLPEDFPYPKMKIIPLDE
                     KRMVARFAPGIEIPLHPFFGSMGVAPPESSGRISSGPPWIHAGNLDNKELVAGTILFI
                     PVHARGALFEVGDGHAGQGNGEVDITALETSLIGTFQFFVRKDLHHRWPRAETPTHFI
                     TMGLHEDLTEATKLAVREMIDFLVTTKHLSRDDAYMLASVAADLSITELVDGNKGVHM
                     MIPKAIFVSLSPK"
     gene            4160..4783
                     /locus_tag="VGR03_17115"
     CDS             4160..4783
                     /locus_tag="VGR03_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996056.1"
                     /translation="MSAAKKQLIARLLALSLFSSLPVNPAAAQSKGERVYTNVVPFIT
                     DGGIPGGPCMRISGDITAPDFFIGLTRISTPDGFEFRRGKELVTEFPQELTLHLQIRD
                     MPCMGSFEAGAPRRFLTQQIVGPLALHFYWKKGFALRPLKNVTRTKASALPLAPLANQ
                     DTGDPVERFVWRFEFAVPSAGVPLTDHLVIVLRTPEGKLVARVSARL"
     gene            complement(4803..>5026)
                     /locus_tag="VGR03_17120"
     CDS             complement(4803..>5026)
                     /locus_tag="VGR03_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996057.1"
                     /translation="RNPRVALSITDPANPYRYLEVRGRVVEITQSGADEQIDRLAQKY
                     LGKAKYPFRKPGEIRVSYRIAPENFSAMG"
BASE COUNT         1062 a         1612 c         1365 g          987 t
ORIGIN      
        1 ggcatcaact ccccatttgg gatcagactg gcgaaagccg agtgtatgtc ctaccgcgaa
       61 aagcagcaga agaacagcag cgattcgata aaacatagat gctttcattc ttgcctccag
      121 agatcacggc agcgggcacc agtatcctgc cgtgtaggtt caaactgtac cactaccgaa
      181 atcctccttg acgggcgttt acttccgtgc tatcatggtc cgtgattatc catccagccg
      241 cgcctcccct acccccgagc agccgtagcc cccttagatt tcccactccc aaagagtcgc
      301 cctgtaagtc atttagaatc aacacttgta aaagtgtatc aaaacaaaga acttcaactc
      361 cctttggaat caacacttac aaaaaacagg ggggaggggg agtatctctt ctgccaatag
      421 tcctggtact ccgctccggg cggaaatggg ccacttcgca cccgaccaga atgccagtcc
      481 cgcttgcccc ggaagcctcc aaggggactg ctcctctcct tccctttctc ggattgctga
      541 taaactggct ccccgaggtg cccatccatg tctgaagcca gacagaccgc aaagaccccc
      601 acgcgcacgg aaaccgacag catgggctcc gtgcaggtcc ccgcggacct ctactacggc
      661 gcgcagaccg cccgctccct cgtccacttc gccatcggca aggacaccat gccgcccgag
      721 ctgatcccgg ctttcggcat cctgaaaaaa gcctgcgcgc tcgtcaacca ggaactcggc
      781 aaactctccg ccgaaaaggc caggctcatc tcgcaggccg ccgatgaagt catcgccgga
      841 aagctcaacg atcatttccc gctgcgcgtc tggcaaaccg gcagcggcac gcagaccaac
      901 atgaacgtca acgaggtcat ctccaatcgc gccatcgagc tggctggcgg cgtcctcggc
      961 agtaagaagc ccattcatcc caacgacgac gtcaacatgt cccagtcctc gaacgacact
     1021 ttccccgccg ccatgtccat cgccgcggcc acggaaacct cgcgccgcct ccttccggcg
     1081 gtgaaaaaac tcggcgacgc cctcaacaag aaggcccgcg aattcgccga gatcgtcaag
     1141 atcggccgca cgcatctgca agacgccacg ccgctcaccc tcggccagga attttccggc
     1201 tgggtcagcc tcctcgaccg cgacgtgaag cgcatcgagc aggccctcga cggcctctac
     1261 gacttggcca tcggcggcac cgccgtcggc accggcttga acgcgcctcc cgaattcggc
     1321 gagcgcgccg cgcgcaagat cgccgagctg agcggcctgc ccttccgctc gcatcccaat
     1381 aaattcgccg cgctctccgc ccacgacgaa atcgtcttcg ccagcggcgc gctgaaaaca
     1441 ctggccgcgt cgctcatgaa aatcgctaat gacgttcgct ggctggcttc cggcccgcgc
     1501 tgcggcctcg gcgagctttc gctccccgaa aacgagcccg gctcctccat catgcccggt
     1561 aaagtcaacc ccacgcagtg cgaggccgtg accatggtcg ccgtgcaggt gatgggcaac
     1621 gacgccgcca tcggcttcgc cggttcgcag ggcaatttcg agctcaacgt cttcaagccc
     1681 gtcatgattt tcaattattt gcactccgtc gagctgctct cggacgcttg cgcgagtttc
     1741 gtggaccact gcgtcgtggg gatcgaggtc aaccgcaagc agattgattt ctacgtgaag
     1801 aattccctga tgctcgtcac cgcgctttcg ccgaaaatcg gctacgacaa agccgccaag
     1861 gccgcgcaca ccgcccacca cgaaaatctc agcctgcgcg aagccgtcat caagctcggc
     1921 tatctttccg gcgaggagtt cgactctctc gtccgccccg ccgacatgac ccatccatga
     1981 atgtctcatt tacacagata accaacgctg tccttttcgt tgcgggctcg ctcgttccaa
     2041 cttttgccag cgcacaagcg tattccgggc caaaatgcgt tggcccagtt tgcatcgatc
     2101 gcaagctccc ctttgagcgt ctggccgtac agctcggagg gccttccagc gggggcggca
     2161 catatggcta tcgaactaaa agtgggcaag cgttcttgat tgttacggaa gacagatggg
     2221 gacaggtcgg ttccatccat ctgagagatt tcgcgaaatg gagaacgtgg cgccaaagag
     2281 accaaaaact cactacagaa gatctgcgtg gctggaaaac cccccaggga atcggtttgg
     2341 gcagtctgga ggaggaggtt ttaagggcat atgggaaacc ctccggcgtg gcaaacttgg
     2401 agctcgaaca tagtcagcgt gagatgggca aaaggatgct catctataaa ggacgattca
     2461 aggagatttt cccagccgct cgatttcgca tccgggatgg aaaagtaagt tggatcgatt
     2521 tggagaacga cgcctttctg gggccggact gcctaggtcc ttattgcacc tatggcgagc
     2581 tgtcgcttaa tagcttgtta cggaagctag gcttgccccc acaaaaaaat acggcctccc
     2641 ctctagagtg ttttcagtca caggacgcgc gagcgtcctt gcactttagg acggacgttg
     2701 agtcgccatc cgatctcgat gacgtgttgc tgagcgactt tcccaattgc ctgcacatgc
     2761 gcaagaggat cgccacaaat ggccttcgcg cctggaagac gccggaaggc atcggattgg
     2821 ggagttcaga agaggatgtc ctgaaggcct atgggacacc ttccgcagag aaagagctgg
     2881 acgcacaaaa ccatggattt gcgatgaagg gtctaattgc cgggtaccga gcaggagaca
     2941 atcaaacacg ggtagggaac aaggtcctca tctacggccc gcgggaactt caagtggtag
     3001 agtttggcat acgcgatgga aaagtatcgt acatctggct gaaagatagt gatttctggt
     3061 tgaaggatgg cgagtagaag gagattgtaa aacactcgga gaccgcatga ttcgtcgcac
     3121 tgtgtttttg agtttttgtt tcctgcctta cctgcacccg ctctgcgctc agcaggtgaa
     3181 cgctcccgcg gcgtacacgc tgaaggcgac gccgaagacg gtggcgtggg gctactacga
     3241 tgccagcacg ccgcctgttt tgcggatcaa gtccggcgac acggttgaga ttcagacgct
     3301 gattaccacc agccccaagc gcctcgaaga cgccggcttg ccgcccgagc aggtcgagca
     3361 gtccctccgc gacatccacg cgcaagtgaa ggacaaaggc cccggcggcc acatcctcgc
     3421 cggacctatt ttcatcgaag gcgccgagcc gggcgacgtc ctcgaagtgc gcatcctcac
     3481 cgcgcgcctc gcgattccct acgcctacaa cgccttcggc cccggccgcg gatttcttcc
     3541 cgaggatttt ccctacccga aaatgaaaat cattccgctc gatgaaaagc gcatggtcgc
     3601 gcgtttcgcc cccggcatcg agattccgct gcatcccttt ttcggcagca tgggcgtcgc
     3661 gccgccggaa tcttccggcc gcatcagcag cggcccgccg tggatccacg ccggaaacct
     3721 cgacaacaag gaactcgtcg ccggcacaat tctcttcatc cccgtgcacg cgcgcggcgc
     3781 gctctttgaa gtcggcgacg gccacgccgg ccagggcaac ggcgaagtgg acatcaccgc
     3841 gctggaaact tccctgatcg gcacgtttca atttttcgtt cggaaagacc ttcaccaccg
     3901 ctggccccgc gccgagacgc ccacgcactt catcaccatg ggcctccacg aagaccttac
     3961 cgaagcgacg aagctcgccg tgcgcgagat gatcgatttc ctcgtgacca cgaagcatct
     4021 ctcgcgcgac gacgcctaca tgctcgcgtc cgtcgccgcc gacttgagca tcaccgaact
     4081 cgtggacggc aacaaaggcg tgcatatgat gattccgaag gccatttttg tttcgctctc
     4141 gcccaaatga tgctcggcca tgagcgcggc gaaaaaacaa ctcatcgctc gtttgctggc
     4201 gctctctctt ttctcctccc tgccggtaaa tcccgcggcg gcccagtcca aaggcgagcg
     4261 ggtgtacacg aacgttgttc cgttcatcac cgacggcgga atcccgggcg gaccctgcat
     4321 gcgcatctcc ggggatatca ccgctccgga tttcttcatc ggcttgacac gcatcagcac
     4381 gccggatggt tttgaattcc gccgcggtaa agaacttgtc actgagttcc cgcaagaatt
     4441 gactctccac ttgcagattc gcgacatgcc ctgtatgggg agcttcgagg ccggcgcgcc
     4501 gcgccggttc ttgactcagc agattgtcgg cccgcttgcc ctgcactttt attggaagaa
     4561 gggtttcgcg ctgcgcccgc tgaaaaatgt cacgcgcacc aaagcatccg ccttaccgct
     4621 cgctccgctt gcgaatcagg acaccggcga tccagtggag cgatttgtct ggcgcttcga
     4681 gttcgccgtc cccagcgccg gcgttccgct taccgatcac ctcgtgatcg tcctgcgaac
     4741 gccggaagga aaactcgtcg cccgggtctc cgcccggctc tgaaaaacta gtctaaacga
     4801 gactagccca tggcgctgaa attttccggc gcgatcctgt agctgacgcg gatttcgccg
     4861 ggcttgcgga agggatattt cgccttgccg agatacttct gcgcgaggcg gtcgatctgc
     4921 tcgtcggcgc cgctctgcgt gatttcaacg acgcggccgc gcacctcgag gtaacggtaa
     4981 gggttcgccg gatcggtgat ggatagcgcc acgcgagggt tgcggc
//
LOCUS       DASYVO010000493         7469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_192263, whole genome shotgun sequence.
ACCESSION   DASYVO010000493 DASYVO010000000
VERSION     DASYVO010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192263"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VGR03_17125"
     CDS             complement(<1..575)
                     /locus_tag="VGR03_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996058.1"
                     /translation="MRRKVQSIAVLSFLAVAPLCAGDRRVHLLPKLQSGQTITYLIRF
                     QSDKTVKTESKVVASMAPNAAQLDAHGLLRIEILDVQEAGSKAAIHARGQFLTLDSGV
                     WLKKPGDKKPDWDIQRVDPSGKSIEFTISPDGSVNNIKGLDSLFPEQQQAWQQWVARF
                     ALAWTLPSDGMKSGEKWMSQQAEQAGAPIAG"
     gene            complement(647..3274)
                     /locus_tag="VGR03_17130"
     CDS             complement(647..3274)
                     /locus_tag="VGR03_17130"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEV1996059.1"
                     /translation="MTKLQKWWKPALAIVFLVIALQAGVSLLARTHRVHAYLVAHLER
                     AFGRQVEVLSFDARILPSPQLDANGVTVGEDPSFGHEYFLRAERLSAGLRWTGLLRGH
                     FEFGTMSLSKPSLILVRNSEGRWNLERWLPPAKVDALQSARSYGPPSPVVPVNRLQKI
                     DFDEGRINFKTGDDKLPFAFTSVSGSVEQISAGRWQLQLEAQPWRSGVSLQSAGTIKV
                     LGDVAGTSTRLQPAEITLHWSEASLADVFRLFRGQDYGLRGVFALDATAKSGTAKEDG
                     PGDWKFSVQGRAGQIHRWDLTERADNPRLNVNVKGRCNVGAGSLVAEELAVEGPRSNL
                     RGMFRLAGGGAPSMELRLDSLGIQASDLLAWYRAFHPGVEEGVTAEQYFTGGMILHGW
                     PLALESAALSSSGGIVKVPGFAEPVRIGPLTGGRERGLLTIGPVRVALGGEAREVVAP
                     KRRRVALAMENAADLTFAHDLNTQSGSISIEGNFFKVEDFLKLSAALGHQLNHGWELT
                     GQATAVTKWEWKKPLDSRWNGSIGFNKANLTVAGLNQPLKISESTLDWIDGRRVARVM
                     RVEGFGGMWTGNIEETSQIDAENGPQWNFHLSVDQLDAADLDRWVGPRARPNWLERLL
                     PTLLGGSAPSTPASELVRRVNAEGELEITKLTIEKLQLEQVVAKGSLHDLQLDVREAQ
                     AEWAGGKVRAKINARFLPRPVYEIAAELDRVNLAQLPGAGRIAERLGGVASGTLQVKT
                     EGVGRDELLGKLAGRGEFRLNKVEFRGWDVNASVADGAEHAGVSRWATGAGSLRLQDS
                     RILLEDFRLDGGKQLTLVNGTLSFGRNADLSIETASALKSKERKAIDSGAGHVLKISG
                     PLDAPKVSVEKAGARQPAD"
     gene            complement(3385..4191)
                     /gene="lepB"
                     /locus_tag="VGR03_17135"
     CDS             complement(3385..4191)
                     /gene="lepB"
                     /locus_tag="VGR03_17135"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV1996060.1"
                     /translation="MPEETVTEIEEQAPEKKQEHRADQTTISEYLESLLVTVILALFG
                     TSFIVQAFKIPSASMEGTLLIGDHLLVNKFIFGGTGAWYEKVLPYRQLERGEIIVFKY
                     PYADHQHFVKRVIGLPGDHMKLVDQEMFINGRPLPEPYVVHDPGAPYDPLNYNFPPPN
                     SQLITSAMQPEWAHEMRKYMHGDEIVVPPAKYFAMGDNRDRSLDSRYWGFVDRDAIMG
                     RPFLIYWSVDAKSADYSGESTFWSRLSGVFETLVHLPSRTRWSRMLHTVH"
     gene            complement(4198..4947)
                     /gene="rnc"
                     /locus_tag="VGR03_17140"
     CDS             complement(4198..4947)
                     /gene="rnc"
                     /locus_tag="VGR03_17140"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV1996061.1"
                     /translation="MQETIRIADGAFEERLGYKFSTRELLERALIHSSAVPELRAAGA
                     EEAVSALLPRDNERLEFLGDAVLELLTSEYLLATFPEWSEGQLSKSRARIVDAGSLEV
                     AARRLRLGEHLRLGRGEEKTGGREKQTLLADAFEAVVAAVYLDGGLGAAREMLRKVLF
                     EQALEERGERISESDRKSALQELLQGRGQAPAEYRVAGESGPDHQKVFQIEVWIDGEC
                     KAAGEGSTKKEAEQRAARSALEQLERAEAKG"
     gene            complement(4973..5854)
                     /locus_tag="VGR03_17145"
     CDS             complement(4973..5854)
                     /locus_tag="VGR03_17145"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1996062.1"
                     /translation="MRKQWNIWLEAVTVAGLLVGMNGGASARGLQHGQGSQSQPPAQQ
                     SDKPKTPEVTPLTLDVPAPVNAEEDAAYKAFQAVNPNDAAKKIETGEAFILKYPESRY
                     KSPVYGALTFAYLQAGNTQKMQEYGEKEIALAPNDVSTLALLAQTLPRSLHGSVSTPE
                     AVQVLAKAEQFSKQAIEITPTLPKPENLTDEAFASAKNQTLAMAHSGLGLVYVRRGKN
                     AEAIPELEQAIKVDPNPDPVNYYLLGMANKATSHFDDAVAALNKCVAMGGPLVGTCKA
                     QADDAKKLGATQLSAPK"
     gene            complement(5952..>7469)
                     /locus_tag="VGR03_17150"
     CDS             complement(5952..>7469)
                     /locus_tag="VGR03_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996063.1"
                     /translation="PAQAPAPRARTRTAPQSTVSVDGSEAMFTTICALLAAGFESNVS
                     SDNWTAFRAQMRERLRQQQGPAVEAVREFYRQHELRDPGATLSRYLWFGLVSGPAPAF
                     QPVLKRDELPPEIIALEGFSEILSNYYQEQKIGQLWRQVQPIYNREVERLHDPVSQIV
                     FVASGYLREILEPSGPRTFAIVVEPLVGRITNVRNFGDHYALVLSGGEEIPSDVVRHA
                     FLHFLLDTLPLRYAHVTAVKRPLFEKAATAPRLAPELKDDYASYFAECTVRAVELKLK
                     RMSPGEREAAMTRDDEDGYLLVKPLFGALPKFENSEPSMKLFFPDLVRAIDMASETKR
                     LAAVKFAPAEEAQTEDQAASEEVARRRNAAPTTVPNDAEVIAALTEGERRIAEKNPRA
                     AEASFQKVLTKYPDQARAWFGIGVVAMLDHDAARAKQVFGRLTVGEHAATEDPMVLVW
                     SHVYLARIYDYEGNSEVAKTEYQSALAVQGGPNQAKQTAQKELATLGAEKPVVRP"
BASE COUNT         1420 a         2503 c         1909 g         1637 t
ORIGIN      
        1 aatccggcga tcggcgctcc ggcctgttcc gcttgctgcg acatccattt ttcgccggac
       61 ttcattccgt cggaagggag cgtccacgcc agcgcaaaac gtgccaccca ctgctgccaa
      121 gcctgctgct gctcgggaaa aagagaatcc aaacctttta tgttgttcac cgagccgtcc
      181 ggagaaattg tgaactcgat gctcttgcca ctggggtcca cacgttggat atcccaatcg
      241 ggcttcttgt cacccggctt tttcaaccac acaccagagt caagagtcag aaactgaccc
      301 cgcgcgtgaa tggccgcttt gctgcccgcc tcttgtacgt ccaagatctc aattcgcagc
      361 aaaccgtgag catcgagctg tgctgcgttc ggcgccatgg acgcgacaac tttgctttcg
      421 gtcttgacgg tcttgtcgct ctgaaaacga atgaggtagg tgatggtttg gccagactgc
      481 agtttgggca ggagatggac gcgacggtcg ccggcgcaga gaggagcaac ggcaagaaac
      541 gacagcacgg caatactttg tactttgcgt ctcatggcat ttgctgctgc tccgcgagtg
      601 ttcgcaaatt tctccgcgtg caatctccgg aaaaaatgtt cggcgtttaa tcagcaggct
      661 gccgggcgcc agccttttcc accgaaactt ttggcgcatc gagcggcccc gaaatcttca
      721 gtacgtgccc agctccagaa tcaatcgcct tgcgttcctt gctttttagc gcgctcgccg
      781 tttcgatcga caagtcagcg tttcgcccga agctgagcgt tccattcacc agcgtcaact
      841 gctttccgcc atcgagccga aaatcctcca gtaagatgcg gctgtcctgc aaacgcagcg
      901 agcctgctcc cgtggcccag cgcgagaccc cggcgtgctc cgcgccatcc gcgacgctcg
      961 cgtttacatc ccacccgcga aactccacct tgttcaaacg aaactcgccg cggccggcca
     1021 gctttcccag gagttcgtcg cggcccacgc cttcggtttt cacctgcaaa gtccccgacg
     1081 ctacgccgcc caatcgctcc gcgattcggc ctgctcccgg cagttgcgcc aggttcacgc
     1141 ggtccaattc cgctgcaatc tcatagacgg gccgcggcaa aaaccttgca tttattttcg
     1201 cgcgcacttt gccgcccgcc cattccgctt gtgcttcacg cacatcgagc tggagatcat
     1261 ggagggaccc cttggccacc acttgttcca gttgcaactt ctcgatcgtc agctttgtaa
     1321 tctccaactc gccttcggcg ttcacgcgcc ggaccagttc actcgcgggc gtgctcggcg
     1381 ccgatcctcc caaaagagtc ggcagcagcc gctctagcca gttcggccgc gctcttggtc
     1441 cgacccaacg atccaaatcc gcagcatcca actggtccac gctcaaatga aaattccatt
     1501 gcggcccatt ttccgcatca atctgagacg tttcctcgat gtttcccgtc cacatcccgc
     1561 cgaacccttc caccctcatt acacgtgcga cgcgccggcc atcgatccaa tccaatgtac
     1621 tctcagatat cttcagcggc tggtttaatc cagcgacagt caaattcgct ttgttgaaac
     1681 cgatgctgcc attccaccgg ctgtcgagcg gcttcttcca ctcccatttc gtgaccgcag
     1741 tcgcctggcc agtcagttcc caaccatgat ttagttggtg accaagcgcc gcggacagtt
     1801 tcaggaaatc ctccaccttg aaaaagtttc cctcaatgct gatgcttcca gactgcgtgt
     1861 tcaaatcgtg cgcaaacgtc aggtccgcgg cgttctccat cgccagcgcc acgcggcgcc
     1921 gttttggcgc cactacctcg cgagcttcgc ctccaagagc aacgcgcacc ggcccaatcg
     1981 tgagcaggcc tcgctcgcgc cctccggtca agggaccgat tcgcaccggc tccgcgaagc
     2041 ctggcacttt cacgattcct ccgctgcttg agagcgctgc cgattcaaga gccagcggcc
     2101 acccgtgcaa aatcatcccg ccggtaaaat actgttccgc cgtcacaccc tcctctacgc
     2161 ccggatgaaa cgcacgatac caggccaaca agtccgaggc ctgtattccc agcgaatcca
     2221 ggcgcaattc catcgatggt gcacctccgc ccgcgagacg gaacatcccg cggagattcg
     2281 atctcggacc ctcgacggcg agttcttctg cgaccaggct ccccgcgccg acgttacaac
     2341 gtcccttcac gttcacattc aggcggggat tatcggcacg ttccgtcaaa tcccagcgat
     2401 ggatctgccc ggcgcgtccc tgcactgaga atttccagtc tccaggtcca tcctccttgg
     2461 cggtcccgct tttcgctgtg gcgtccagcg caaacactcc gcgcaaaccg tagtcttgtc
     2521 ctcggaagag ccggaagacg tctgccaacg acgcttcgct ccaatgaaga gtgatttccg
     2581 cgggctgcaa gcgcgtcgaa gttcccgcca cgtcaccaag cactttgatc gttcccgccg
     2641 attgcagcga aactccactc cgccagggct gggcctccag ttgcaattgc cagcgaccag
     2701 ccgagatctg ctccacactc cccgaaacac tcgtgaacgc aaagggcaac ttgtcatccc
     2761 cggtcttaaa attgattcgc ccttcatcga agtcgatttt ttgcagccgg ttcactggaa
     2821 cgacgggtga gggcggtcca taggagcgcg cgctttgcag agcatcgact tttgccggcg
     2881 gcagccacct ctccagattc cagcggcctt ccgaattccg caccagaatc aggctcggct
     2941 tgctgagcga catggttcca aattcaaaat gcccccgcag cagaccagtc cagcgcagac
     3001 cggcgctcag gcgttccgcc cgaagaaagt attcgtggcc gaacgagggg tcctctccca
     3061 cggtcacacc gttagcatcc agttgcggac tcggcagaat ccgcgcgtcg aaactcagga
     3121 cttccacctg tcgtccaaac gctcgctcca gatgcgctac caagtaggca tgcacgcggt
     3181 gggtgcgcgc cagaagagaa acaccggcct gcaacgcgat gaccaggaat acgatcgcca
     3241 gcgccggttt ccaccacttt tgcagcttgg tcatcttgct cggaagtttc gcagactcat
     3301 gcgcaaaaag gtagcggcga gcctgtaccc tgaacgtact gaagagtttg cttgcaggtg
     3361 ttttctctat cctgaatcgg aatcctaatg cacggtatgg agcatccggc tccatcgtgt
     3421 tcgagacggc aaatgcacca gcgtctcgaa aacgccggac aagcgcgacc agaaggtgct
     3481 ttccccgctg tagtcggcgc ttttcgcatc caccgaccag taaatcaaga acggccgccc
     3541 catgatggca tctcgatcga cgaatcccca gtagcggctg tccaagctgc ggtcgcgatt
     3601 gtcgcccatc gcgaagtatt ttgcaggcgg cacgacgatt tcatctccat gcatatactt
     3661 ccgcatttca tgcgcccatt ccggctgcat cgcggaggtg atcaattggc tgttcggcgg
     3721 aggaaagttg taattcaagg ggtcataggg ggcgccggga tcgtgcacga cgtagggctc
     3781 gggcagcggc cttccgttga tgaacatctc ctggtcgacc agcttcatat gatctcccgg
     3841 caaaccaatc acgcgcttca cgaaatgctg gtgatcggca tagggatact tgaaaacaat
     3901 gatctcgccc cgctcgagtt gccggtaagg caacactttt tcgtaccagg ccccggttcc
     3961 tccgaaaata aacttgttca ccagcaaatg gtcgccgatc agcagggtgc cttccatcga
     4021 tgccgatgga atcttgaaag cctgtacgat gaaacttgtg ccgaacagcg ccagaatcac
     4081 ggtcaccaga agtgactcca ggtattcgga aatcgtggtc tggtcggcac ggtgttcctg
     4141 cttcttttcc ggcgcctgtt cttcgatttc tgtcactgtc tcttccggca ttccgcctca
     4201 gcctttcgct tccgcgcgtt cgagttgctc caaagcgctc cgtgcggcac gttgctccgc
     4261 ctcttttttc gtgctgcctt cgcctgccgc cttgcactcc ccatcaatcc aaacttcgat
     4321 ttggaaaacc ttttggtgat cggggccgct ctctccggcc acgcgatatt ccgccggcgc
     4381 ctgccctcgt ccctgcaaga gttcctgcaa tgcggatttg cgatcggact ccgagatgcg
     4441 ctcaccgcgc tcctcgagcg cctgctcgaa caggaccttt cgcaacatct cgcgcgccgc
     4501 acccagtccg ccatcgaggt aaaccgcagc gactaccgct tcgaacgcgt cggccagcag
     4561 cgtctgcttc tcgcgaccac cggttttctc ttctccgcgc cccaacctca agtgctcccc
     4621 caggcgcagc cgccgcgcag ccacctccag ggagcctgca tctacaatcc gcgcgcggct
     4681 cttggaaagc tgcccttcgc tccactccgg aaaagtcgcc aaaagatact cgctcgtgag
     4741 cagctcgagg acagcatctc cgaggaactc cagccgctca ttgtcccggg ggagcaaggc
     4801 cgagacagct tcttcggcgc cagctgcgcg caattcggga acggctgaac tgtgaatcag
     4861 cgccctctcg aggagttctc ttgtcgaaaa cttgtacccc agacgctcct caaacgctcc
     4921 gtccgcgatc cggatagtct cttgcataat tccgccgtcc cggcggcctg gctcacttcg
     4981 gcgcgctcag ttgcgtcgca ccaagcttct tggcgtcgtc tgcctgggcc ttacaggtcc
     5041 ccaccagggg cccccccatc gctacgcact tgttgagagc cgccaccgca tcatcgaagt
     5101 gcgacgtggc tttattcgcc atccccaaca agtagtagtt cacagggtcc gggttcggat
     5161 cgacctttat cgcctgttcc agttccggaa tcgcttcggc attctttcct cgccgcacgt
     5221 agaccaaccc caacccgctg tgggccatcg ccagcgtctg gttcttcgcg gacgcaaaag
     5281 cttcatcggt aaggttctcg ggcttcggga gcgttggcgt aatttcgatg gcttgcttgg
     5341 aaaattgttc cgctttggcc agcacttgca cggcttcggg tgtacttaca ctcccatgca
     5401 agctccgagg cagcgtctgc gcaagcagcg cgagcgtgga gacgtcgttg ggcgccaacg
     5461 cgatttcctt ttctccatat tcctgcatct tttgcgtgtt tccggcctgc aggtacgcaa
     5521 aagtcaacgc cccgtatacg ggagatttgt agcgactctc gggatatttc aggataaatg
     5581 cttcaccggt ttcaatcttc ttggcagcat cattgggatt cacggcctga aaggccttgt
     5641 acgcggcatc ttcttccgcg ttcaccggcg ctggcacgtc cagcgtcaga ggtgtaacct
     5701 ccggggtctt cggtttgtcg ctctgctgcg ccggaggctg gctctggctt ccctggccat
     5761 gttgcagacc gcgcgccgat gcgcctccgt tcatgcctac cagcagcccc gcgacggtta
     5821 ccgcttccaa ccaaatattc cactgctttc tcatcctttt tctcctcttc ccttcttgct
     5881 tcactcaaga ctcgtaaatg gagccacctc ggctcatcag ccaactccgc ccagcgaaaa
     5941 tcgaccttcg ttcacggccg gactacgggc ttctccgcgc ccaacgtggc caactctttt
     6001 tgtgcggtct gcttcgcctg gttcggtccg ccctgcactg ccagggcaga ttgatactcc
     6061 gtcttcgcca cttccgaatt cccctcataa tcataaatcc gagccaggta aacgtgcgac
     6121 cacacgagta ccatgggatc ctctgtcgcc gcatgttcac cgacggtcag gcgcccgaat
     6181 acctgcttgg ctctcgcggc atcgtgatcc agcattgcca cgaccccaat tccgaaccat
     6241 gcccttgcct ggtctggata cttcgtcaaa actttttgga atgacgcttc tgccgcgcgc
     6301 ggatttttct ccgcgatccg ccgctctccc tccgtcagcg ccgcaatgac ttctgcatca
     6361 tttggcaccg tcgttggcgc cgcgtttctc cgccgcgcca cttcttcact cgccgcttgg
     6421 tcctcagtct gagcctcctc ggccggcgca aatttcaccg ccgccaaccg cttggtctca
     6481 gatgccatat cgatggctct caccaaatcc gggaaaaaga gcttcatcga cggttcggaa
     6541 ttttcgaatt tgggcagcgc cccgaagagc ggcttcacca gaaggtaccc gtcttcatcg
     6601 tcccgggtca tcgccgcttc ccgttcaccg ggcgacatgc gcttgagttt caactccacc
     6661 gcgcgcaccg tgcattccgc aaagtacgac gcatagtcgt cctttaactc cggcgcaagc
     6721 cgcggagcgg tggcggcctt ctcaaatagc ggacgtttca ccgccgtcac gtgggcatac
     6781 ctcaggggca gcgtgtccag caggaagtgc aaaaaggcat gacgtacgac gtccgatgga
     6841 atctcttctc ctccgctcag caccagcgca tagtgatccc caaaatttct tacgttggtt
     6901 atgcgcccca ccagcggctc gacgacgatc gcaaatgtcc gcggcccgga cggctcgagg
     6961 atttcacgca gatatcccga cgcgacgaac acaatctgcg acaccggatc gtgcagccgc
     7021 tcgacttcgc ggttataaat cggctgcact tgccgccata gttgcccgat tttctgttcc
     7081 tgataatagt tcgaaaggat ttcgctgaaa ccctcgagcg ctatgatttc cggcggcaac
     7141 tcatcccgct tcaacactgg ctgaaacgcg ggcgcgggtc cggacaccag gccaaaccac
     7201 aaataccttg aaagcgtcgc cccgggatcg cgcagctcgt gctgccggta aaattcccgc
     7261 accgcttcca ccgcgggtcc ctgttgctgg cgcaggcgct cgcgcatttg cgcgcggaac
     7321 gccgtccaat tgtcggagct gacgttcgat tcgaaacccg ccgccagcag cgcgcagatc
     7381 gtcgtaaaca tcgcctcgct gccgtcgaca ctcaccgtgc tctgcggagc ggtgcgcgtg
     7441 cgcgctcgtg gcgcaggggc ctgcgccgg
//
LOCUS       DASYVO010000494        12405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_209493, whole genome shotgun sequence.
ACCESSION   DASYVO010000494 DASYVO010000000
VERSION     DASYVO010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 12405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12405
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209493"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VGR03_17155"
     CDS             complement(<1..857)
                     /locus_tag="VGR03_17155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1996064.1"
                     /translation="MPTLPANENNRLSPPINRVIALLALSALINYIDRGNLSIAAPLL
                     KGELGLSFSQLGILLSSFFWTYSLFLPVSGWLADRFDAKWVMAFGFFLWSGATAITGG
                     LHAFTALLAARLMLGIGESVAYPCYARTLVRHVPEVGRGFANALIAAGVGCGPALGTF
                     FGGTLMARYGWRPFFIILGVISMAWLIPWFLWVPRGGAAISPAEKSGAWFLKLLMQRS
                     MWGTCGGLFGANYVLYFEITWLPIYLVHERHFSMGRMAKIGGVGYLCYSAAAVAFGWI
                     SDRWIAAGGS"
     gene            complement(909..2867)
                     /locus_tag="VGR03_17160"
     CDS             complement(909..2867)
                     /locus_tag="VGR03_17160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV1996065.1"
                     /translation="MKSLRSTCLSFWVLFVCGSAVAQSPGPDTRQISDPKTIVSASNP
                     NARAIPIDDLYFTRGVSNASWSPDGKEIVFTTDISGRSNLWKVNADGGWPIQLTQSDE
                     RQYGETWSPDGKWIVFQQDTGGNELWDVFAIPSDGGEAINLSKTPEIREESPRWSPDG
                     KTIALNTKPKDGTVYDITLIDWATRKMTPLTHESTPNHLWQSVAWSVDGKTLYANRLE
                     VSFTDADIYAIDAASGKATNLTAHQGLVLNIASSLSHDGKTLLITCNEKGGYQNVALL
                     DIAGRKLAWVTDTKWEASSGDFSPDGKRLTYTINADGRVDTYVADAATMRSKKIPVAG
                     GVNGFAAYPNSFSPSGDRLLLSHESSVEPGDFWVYDVTSRKSKQLTYSAIASLSSAPR
                     APSQVISYKSFDGKTISALLWIPFNLKRDGSNPALVLPHGGPTGQSGDYWSPRVAAFV
                     SRGYVCIEPNVRGSTGYGMEFQKANYKDLGGGDLQDEVYAAKFLEATGYVNPKKIGIT
                     GGSYGGFMTLMAIGRTPDNWAAGVEMYGIINWMTMLEHEDGMLQQYEKSLLGDPVKDR
                     ASYDAASPITYIHSVKAPLLVLQGDNDPRVPKEEAEQVVALLKKDGKTVDAHYYANEG
                     HGFEKRENQIDSIRRTVAWFDKYLKGIN"
     gene            complement(2998..3423)
                     /locus_tag="VGR03_17165"
     CDS             complement(2998..3423)
                     /locus_tag="VGR03_17165"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEV1996066.1"
                     /translation="MHFHSLNLLAVLVAAISTMVVGFVWYSPLLFAKAWMREMGYDPN
                     DKAKMEEMKKSAGPAYGGSFLASLVSAFVLALLLHGLRGEGLHFGVVVGFHVWLGFVA
                     TVQLTGALFMKQSMKLFAINTGYQLVCYLAMGAILAVWR"
     gene            complement(3557..4039)
                     /locus_tag="VGR03_17170"
     CDS             complement(3557..4039)
                     /locus_tag="VGR03_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996067.1"
                     /translation="MARPKLAANPKRQLDSFLAKYDPAIAAYARRVLVKLRKRLPGAV
                     EMVYDNYNGLVIGFGPNDRPSLAIFSIVLFPRWVTLCFLQGAGLPDPQRRLQGQGRVV
                     RHIRLEDSSVLDDAYVQRLMNLVLHRAKVPLDPKNRRQLIIKSISAKQRPRKPTPERK
                     "
     gene            complement(4100..4648)
                     /locus_tag="VGR03_17175"
     CDS             complement(4100..4648)
                     /locus_tag="VGR03_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996068.1"
                     /translation="MARFAKYLVLLSVLLLTFSIWHLQTTMAGNPPAEGKEVVLKAAD
                     ITPKIFPERVFFRGQSAPVQFRNSGGVHFADDSYVLAGLVDSSGYSTGIKEKYQAYLL
                     NEVTLEIAGQTLKPGAYGIGFLTGGKFVVMDVGANDVLQAASQRDAEMKRPVPLQVLA
                     SATAGSYRLYAGRDYVEFRRVH"
     gene            complement(4798..5652)
                     /locus_tag="VGR03_17180"
     CDS             complement(4798..5652)
                     /locus_tag="VGR03_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996069.1"
                     /translation="MKKRLFFCLLAMLLSCISSAAQQAQPQPLTFWYVYTIQPGKEDE
                     FMDVVKSVGQPVRDKLMADGVVLAWGVQASMLRVPGNGTHWIWYVVADWSGLEKVDTA
                     MRAQIAKMSSSDSMAPAGKKGQKPSASVYERLGQIADLSKAHDYVTRSLVSNESSTTP
                     AGALPYTRYGFVKVKPGKGGDYRKAWEKYNKPVYDKLVADGVILGYGLDVEEVRTSGE
                     FTHFTWVDTKDLAAMDKLRAAFMADRDRRTQEEQDAITNLFTSLLDADASRGEIARSL
                     IFHVPAPK"
     gene            5904..7781
                     /locus_tag="VGR03_17185"
     CDS             5904..7781
                     /locus_tag="VGR03_17185"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV1996070.1"
                     /translation="MLDRRNFMKTCSGMGLAGTLFPGVLWAQAQAQGAKKITKEMIEN
                     AAAVADVPIAEDYKGMMLESLNDQAKGYEEIYKLHIPNSVDPALIFDPVLPGMKFETE
                     RKQTRMSAPNLPEAFSNFGRAGLRPNYEALAFASVRELAEMVRTKRLSSMSLTVMYLQ
                     RLKRLDPTLKFVVTLTEERALAQAKKADAEIAAGKYRGPLHGLPWGAKDLLAVKGYRT
                     TWGAGGFESQMIDDDATVVKRLDEAGAVLVAKLTLGALALGDAWFGGVTRNPWNTNQG
                     SSGSSAGPASATAAGCVAFSIGSETLGSISSPSTRCGCTGLRPSFGLVPRTGAMALSW
                     SMDKLGPICRTVEDCALVLDAIHGRDGKDRTVQPAAFNWDANLDWRKLRVGYLKSAFE
                     RKPDDQQEAAKEEPAATPEEQKKREEQKKRREAARARAEYDRKYNDAALAKLREMGVN
                     LLPVEMPKFPYDAMVTMLTAESAAAFDELTRTGKDKLLTSQKDYDWPNTFRTARFIPA
                     VEYIQAARARRMAMDAVARVFHDLDVIVAPTSGEQLVIANLTGHPAVILPNGLRGSDA
                     PKPPSDDVSDFQNAGGPGTPTSLTFLGKLYGEAELLAFARAYQEATGFHLLHPQLPPA
                     S"
     gene            complement(7812..8318)
                     /locus_tag="VGR03_17190"
     CDS             complement(7812..8318)
                     /locus_tag="VGR03_17190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996071.1"
                     /translation="MAPATSRFQTLFLVSALTVLFAVSATLVSVPAIRGDEKQSSDPW
                     TSAQVLKADDFAREVSDKNGPSSTIVYVGFRTLFAGGHISGASFHGSASTEQGLAELK
                     KWADGLPRTTNLVIYCGCCPFDKCPNFRPAFAALSNMGFKKLRVLVLPTNFATDWVDK
                     GYPIQKGM"
     gene            complement(8385..9356)
                     /locus_tag="VGR03_17195"
     CDS             complement(8385..9356)
                     /locus_tag="VGR03_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996072.1"
                     /translation="METLSPEVLEDLRHGRATRERKIAVCTGGAHLAPADHAEILAVL
                     AHDADEMVSARAQDAILSQSVESFVEALKREHALPALFSYAAKNLADKPGVCDAMVQN
                     KNCSAEHLVPLVRHLSTLGIQALMEELDRISESPALASALEHSSSVTVEQKSQLHELH
                     GPDNVIDEAALAEAAAAAESDVARRQTLIQRLAKMTVAQRVQFAMKGGPEARRTLIRD
                     NNKVVQRAVLQSPRLTDQEVEAFAAMSSLTDEVLRLIAGNRNFRKNYSVVRNLITNPK
                     TPLDVTMHMLPMLNVVDLKRLTSNKNVPETLRTTATKLQRTRAELKK"
     gene            complement(9361..10104)
                     /locus_tag="VGR03_17200"
     CDS             complement(9361..10104)
                     /locus_tag="VGR03_17200"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEV1996073.1"
                     /translation="MAKGNFGERLKREREMREVSMDELTKATRISTRFVQALENEDWA
                     KLPGGVFGHGFVRTIARYLGLNEESLLGEYDLARAEHLAAHPPKTEERIPPPPKWLPA
                     LAVLLLLLLLAGLFYAGRYGWHRYAAYRATKKSAAASLPMQPHPGSTSPGSAEPFSSV
                     ASAAALLDLSVSTSAATRVRILADNKLLLDAELPAGETLHFPANDHFEVTAGDSSAVL
                     LELNGKAMPPLGAPGASGRIVLSEMDLRP"
     gene            11234..12274
                     /locus_tag="VGR03_17205"
     CDS             11234..12274
                     /locus_tag="VGR03_17205"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV1996074.1"
                     /translation="MMLIGCDYHPSWQQICWLDTATGEAGEKKLEHASGEAEKFYRGL
                     PGAALIGMESTGNCQWFVEMATTLGHEVLVGDAAKIRASEVRQQKHDRRDAALILKLL
                     REGRFPQIWTPSGEEKDLRQLLIHRYKLVRIRAQVKTELQHLAMNQGITKKRKLWSKA
                     GEKVLRELPLKPWASRRREDLFRVREMLNGQIDLLDQAVVEAAEKNEKARLLMTQPGV
                     GPITSLAFVLTMGDVKRFQRGKQVASYLGLIPREYSSGGKQRLGSISKQGNRFMRMLL
                     VEAAQGAVRYDPGFRNEYLHRCHTKPKGVAKVAAARKLAVRLYWMLRTNTGYPEIVRV
                     ESSSRVPLVSES"
BASE COUNT         2725 a         3319 c         3834 g         2527 t
ORIGIN      
        1 gatccaccgg cagcgatcca ccggtcggag atccacccga acgccactgc ggcagccgag
       61 taacacaggt atccgacgcc gccaattttc gccatccttc ccatcgaaaa atggcgctcg
      121 tgcacgagat aaatcggcag ccaggtgatc tcgaagtaca gcacgtagtt cgcgccaaaa
      181 aggcccccac aggttcccca catcgatcgc tgcatcaaga gtttgagaaa ccatgcgccg
      241 cttttctccg cgggcgaaat ggccgctcct cctcgcggca cccataggaa ccagggaatc
      301 agccaggcca tgctgatgac cccgaggatg atgaagaagg ggcgccatcc gtagcgcgcc
      361 atgagcgtcc ccccgaaaaa tgtacccagg gcgggaccgc atccaactcc cgcagcgatt
      421 aaagcgtttg caaatcctcg gccaacttcc ggaacatgcc gcaccagcgt cctcgcatag
      481 cacggatagg cgaccgattc gccgatcccg agcataaggc gcgcggcaag caatgccgtg
      541 aatgcgtgca aaccgccggt gatggccgtc gcgccggacc agaggaaaaa accaaatgcc
      601 atcacccatt tagcatcaaa acgatccgcc agccagccgg aaaccggcag gaaaagagag
      661 taggtccaaa aaaaagagga cagcaggatg ccgagctgcg agaacgataa tcccagctca
      721 cccttcagca aaggcgcggc gatggagagg ttgccgcgat cgatgtaatt gatcagggcg
      781 gagagcgcca gcagcgcgat cacccggttg atcggaggcg aaagcctgtt gttctcgttg
      841 gcgggaaggg tgggcaagtg ggaacgccgg atattctctc ggtccggagg cctcttcatc
      901 cgtgcgaatc agttgattcc cttgaggtat ttgtcgaacc acgctaccgt gcgccggatc
      961 gaatcgattt ggttctcacg tttctcgaag ccgtgccctt cgttcgcgta gtagtgtgca
     1021 tccaccgtct ttccatcttt cttcagcagc gcgacaactt gctccgcctc ctctttagga
     1081 acgcgtgggt cgttgtcccc ctgcaagacc agcagcggcg ccttcacact gtgaatataa
     1141 gtgatgggag aggcggcatc gtaagaggcg cgatctttca ctggatcacc cagcaggctc
     1201 ttttcatatt gctgcagcat cccatcctca tgttccagca tcgtcatcca gttaatgatg
     1261 ccgtacatct ccacgcccgc tgcccagttg tccggagtcc tgccgatcgc catgagcgtc
     1321 atgaatccgc cgtaggaacc gcccgtgatc ccgattttct tcggattcac gtaaccggta
     1381 gcttccagga acttggccgc gtaaacctca tcttgtaaat ctccgccgcc caggtccttg
     1441 tagttagcct tttgaaactc cattccatac ccggtcgaac cgcgcacgtt gggctcgatg
     1501 cagacgtatc ctcgggacac gaacgcagcc acacgcggac tccagtaatc tccagactga
     1561 cccgttggcc cgccgtgcgg caagacaagt gccgggttcg acccgtcgcg tttcaggttg
     1621 aagggaatcc atagcagcgc gctgatcgtt ttcccatcga atgacttgta gctgatgacc
     1681 tgtgatggtg ctcgcggcgc ggagctcaag ctcgcgatgg cggagtaggt aagctgcttt
     1741 gatttacgcg atgtcacgtc gtaaacccag aaatctcccg gctccacgga gctctcatgc
     1801 gacaacagca gccggtctcc ggacggcgaa aaggagttcg ggtaggcggc gaaaccgttc
     1861 acgcctccag ccactgggat tttctttgaa cgcatcgtgg ccgcgtctgc cacgtatgtg
     1921 tctacgcgcc cgtccgcgtt gatcgtataa gtaagccgct tcccgtcggg agaaaagtcg
     1981 cccgaactgg cctcccactt ggtgtccgtg acccacgcaa gctttctccc cgcgatatcc
     2041 aggagcgcca cgttttggta gccacccttc tcgttgcaag taatcaacag tgtcttccca
     2101 tcatgtgaca gcgaagatgc gatgttgagc accagccctt gatgcgcggt caggttcgtg
     2161 gcttttccgc tcgcggcgtc aatcgcgtag atgtcggcgt ccgtaaagct cacttccagg
     2221 cggttggcgt atagtgtttt tccgtcgaca ctccatgcca ccgattgcca aagatggttt
     2281 ggagtgcttt cgtgggtaag tggcgtcatc ttccgcgtgg cccagtctat cagcgtaatg
     2341 tcgtacaccg tgccatcctt cggcttggtg ttcagcgcga tggtcttccc gtcgggcgac
     2401 catcgagggc tctcttcgcg aatctcggga gtcttgctca ggttgatggc ttcgccgcca
     2461 tcgctcggga tggcgaacac atcccacaat tcgtttccgc cggtgtcctg ttgaaagacg
     2521 atccatttgc cgtcgggaga ccaggtttcg ccatactgcc tttcgtcgga ctgcgtcagt
     2581 tgtatcggcc atcccccgtc cgcgtttact ttccacaaat tcgatcggcc ggaaatgtcc
     2641 gtggtgaaga caatctcttt gccgtcggga gaccatgacg cgttggaaac gccgcgcgtg
     2701 aagtacagat catcaatcgg aatggcgcgc gcattcggat tggacgccga gacgattgtt
     2761 tttggatcgc tgatttgccg cgtatccggg cctggcgact gggcaacggc agaaccacaa
     2821 acaaagagaa cccagaaaga aagacaggta ctccgcagag atttcatcgt gacctcgatt
     2881 ccagctggca tctcgccagc attctgcctg gactctcgaa cccgtggcat tgcacaccca
     2941 taggccatca gaagattgcg cacttcgcgg aacggtctct cctgcttcag catttcttta
     3001 cctccaaacg gcgaggatcg cccccatcgc gaggtagcag acgagctggt aaccagtatt
     3061 gatagcgaag agcttcatgc tctgcttcat gaacaacgca cctgtcaact gcacggtggc
     3121 cacgaagcct agccacacgt ggaagccaac aaccacgccg aagtgcaggc cctccccgcg
     3181 aagcccgtga aggagcaggg ccaagacaaa tgcagagacc agactagcta ggaaggaacc
     3241 gccgtaagcc ggaccagcgc ttttcttcat ctcctccatt ttggctttgt cgttcggatc
     3301 gtagcccatc tcgcgcatcc aggcttttgc gaacagcaga ggcgagtacc acacaaatcc
     3361 cacaaccatg gtggaaatgg ccgcaacaag aaccgcaagc aggttaagac tatggaagtg
     3421 catgaatgat ttcctccgtt tttgcagtga gggaaacttt gcgcgttatc ttagggtgcg
     3481 ttgccgttgg atgtaaagga aaagaagttt gcagaggcgc gaacggagat gggaattgcc
     3541 ggcaccgaag tgagaattac ttgcgttctg gcgtaggctt gcggggccgc tgcttggccg
     3601 agatcgactt gatgatgagc tggcggcgat tcttgggatc gagaggcact ttcgcgcggt
     3661 gcagtaccag gttcatcagc ctctgaacgt aggcgtcgtc cagcacagag gagtcttcca
     3721 atcggatgtg ccgcaccact ctgccctggc cctgaagacg ccgttgcggg tccgggagcc
     3781 ccgctccctg aaggaagcac agagtcaccc aacggggaaa cagcacgatc gaaaaaatcg
     3841 ccagcgaggg tcggtcgttc gggccgaaac cgatcaccaa accgttgtag ttgtcataaa
     3901 ccatctcgac ggcgccagga agccgcttgc gcagcttgac cagcacccgt cgcgcgtacg
     3961 ccgcgatcgc cggatcgtac ttggctagga acgagtcgag ctgtcgtttt ggattcgccg
     4021 cgagtttggg ccttgccatt gccattgagc tgccgtgaag aattcggccc gggctttttt
     4081 tcaaagacga atatctgggc tagtggaccc tgcggaactc cacgtaatcg cgcccggcgt
     4141 agagccggta actgcccgca gtggcagagg caagcacttg cagcggaacg gggcgcttca
     4201 tctcggcgtc gcgctgcgag gcggcttgca agacgtcatt ggcgccgacg tccatcacca
     4261 cgaactttcc accggtaagg aaaccgatgc cgtaagcgcc gggcttcaaa gtttgaccgg
     4321 cgatttccag cgtaacttcg ttgagcagat aggcttggta tttctccttg attcccgtgg
     4381 agtagccgga gctgtccacg agtcccgcga gaacataaga atcgtcagcg aagtggacgc
     4441 caccgctgtt gcggaactgc accggcgcac tctggccgcg gaagaaaacg cgttccggga
     4501 aaattttcgg agtgatgtcg gccgccttga gaacaacttc cttcccctca gcgggaggat
     4561 taccggccat cgtcgtttgc agatgccaaa tggagaaagt cagaagcagc acagacaaga
     4621 gaaccaagta ttttgcgaag cgcgccatag aggctccttt caagatgcgt tcatcctaca
     4681 acatttctct tttccacagc agacgatccc ccctgcgtgt ccccgctgcg agaaaaatcc
     4741 ggccgaaaat ggagagggcc ggccatgagc cggcccttct tccgggatag tggcgcctta
     4801 cttgggggcg gggacgtgga agatcaagga gcgagcgatc tctccacgcg aagcatccgc
     4861 atcaagcaga cttgtaaaga gattggttat tgcatcttgc tcttcctgag ttcggcggtc
     4921 gcggtcggcc atgaaggcgg cgcgcagctt atccattgcc gccagatcct tcgtgtccac
     4981 ccaagtgaag tgggtgaact cgccgctggt gcgcacttct tccacatcca ggccatatcc
     5041 gagtatcaca ccgtcggcga cgagtttatc gtagaccggc ttgttatatt tttcccatgc
     5101 tttgcgataa tcgccgccct tgccgggctt gaccttcacg aagccgtagc gcgtgtaggg
     5161 tagtgctcca gcaggtgtcg tagaggattc attcgaaacc aggctgcgcg tgacgtagtc
     5221 atgcgccttg ctcaggtcgg caatctgccc cagccgttcg taaaccgacg cagagggttt
     5281 ctggcccttt tttccggcag gcgccatgga atcacttgaa ctcatttttg cgatctgtgc
     5341 gcgcatcgcg gtgtcaactt tctctagccc cgaccaatcg gccacgacgt accagatcca
     5401 atgggttcca ttacccggga cacggagcat ggaagcctgg accccccagg ccaacaccac
     5461 gccgtcggcc atgagtttgt cgcgcacggg ttgccccact gacttgacca cgtccatgaa
     5521 ctcatcttct ttgcctggct ggatcgtgta tacgtaccag aaggtgagcg gctgaggttg
     5581 cgcttgttgc gcagcgctcg aaatacaaga caacaacatt gcgagtagac agaaaaacag
     5641 gcgcttcttc atagtcggtt ctctccttga aaagtttctg cctgcactgg gaccatggcc
     5701 agtttgtgtt cgtagatgaa actcagacgt tgcgggcagg cgctcaacat ccgtgcttcg
     5761 aaggggccga cctggtcaag aatgagtttc cttttagcat cagcactgag gcgcgtcaag
     5821 gaatttgcag tgtcacggag agcttgcaag tgtggggatg gtatattgca cgcctgcgtg
     5881 atcgcactcg ggggaggaca tccatgctgg atcgccgtaa tttcatgaag acctgttcgg
     5941 gaatgggtct cgcgggaacg ctctttccgg gagtgttgtg ggcgcaagca caagcgcagg
     6001 gcgcgaagaa aatcacgaaa gaaatgatcg agaatgccgc ggcggtcgcg gatgtgccga
     6061 tcgcggaaga ctacaaggga atgatgctcg agagcttgaa cgatcaggcc aagggctacg
     6121 aagaaattta caaactgcac ataccgaatt ccgtcgatcc ggcgctgatc tttgatcccg
     6181 tgctgccggg aatgaagttc gaaaccgaac gaaagcagac gcgcatgtcg gcgccgaatc
     6241 tcccggaggc cttcagcaac tttgggcgtg ccggactgcg gccgaattat gaggcactgg
     6301 cgtttgcgag tgtgcgggag ctggcggaga tggtgcggac aaagagattg tcttcgatgt
     6361 ctctgacggt gatgtactta cagcggctga agcgtcttga cccgacgctg aagtttgtcg
     6421 taacactaac ggaagaacgc gcgctcgcgc aggctaaaaa agcagacgcg gaaattgctg
     6481 ccggaaaata ccgcgggccg ctgcacggct tgccgtgggg cgccaaggat ttgctggcgg
     6541 tgaagggcta caggacaacc tggggcgctg gcggattcga aagccagatg atcgatgacg
     6601 acgcaaccgt ggtaaagcgc ctggacgaag cgggcgcggt gctggtcgcg aaattaacgc
     6661 tgggtgcact ggcgctgggc gacgcgtggt tcggcggcgt gacgcggaat ccctggaata
     6721 cgaatcaggg atcgagcgga tcgtcggcgg gaccggcttc ggcgaccgcg gcgggatgcg
     6781 tggcgttcag catcgggtcg gagacgctgg gctcgatttc gtcaccgtcg acgcgctgcg
     6841 gttgcacggg actgcggccg tcgttcggtc tggtgccgag aacgggagcg atggcgctct
     6901 cttggagcat ggacaagttg gggcccatct gcagaactgt ggaagattgc gcgctcgtcc
     6961 tggacgcgat tcatgggcga gatggcaagg atcgcacggt acagcccgcg gctttcaatt
     7021 gggacgcgaa tctggattgg cgaaagctcc gcgttggtta cttgaaatcc gcattcgaac
     7081 ggaaacccga cgatcagcaa gaggccgcaa aggaagaacc agccgcgact cccgaagaac
     7141 agaagaagcg ggaggagcag aagaagcggc gcgaggcagc gcgtgcacga gcggagtacg
     7201 atagaaaata caacgacgca gccctggcga aactgcgaga aatgggagtg aatctcttgc
     7261 cggtcgagat gcccaaattt ccctatgatg ccatggtaac gatgctgacg gcggaatcgg
     7321 cggcggcttt cgatgaactg acgcgcacgg ggaaagacaa gctgctaacc tcgcagaaag
     7381 attatgactg gccgaacacc tttcgaacgg cacgcttcat ccccgctgtt gagtacatcc
     7441 aggccgcccg tgcgcggcga atggcgatgg atgcggtcgc aagagtgttc cacgatcttg
     7501 acgtaatcgt ggcgccgacg agcggcgaac aacttgtgat tgccaatctg actggacacc
     7561 ccgccgtgat tttgccgaac ggattgcgcg gcagcgatgc gccgaagccg ccatccgacg
     7621 atgtgagcga tttccagaac gcgggcggcc cggggactcc caccagcttg acgtttctcg
     7681 ggaaattgta cggcgaggcg gagctgctcg cgtttgcgcg cgcctaccag gaagcaacgg
     7741 gattccatct cctacatccc caactgccgc cggcgtcctg atcgaatttg aatgtccagc
     7801 ggctggtggg gttacattcc tttttgaatg gggtagccct tgtcgaccca gtccgtggcg
     7861 aaatttgtgg gaagaacgag gacgcgcaat ttcttgaatc ccatgttgct caaggccgcg
     7921 aacgccggac gaaagttggg acacttgtcg aacgggcaac agccgcaata gatgaccaga
     7981 ttcgtcgttc gcggcagacc gtccgcccac tttttcaact ccgctaatcc ttgctcggtg
     8041 gaagcggagc catgaaacga agcgcccgag atatggccgc cagcgaagag cgtgcggaag
     8101 ccgacataga caatcgtgga agaaggccca ttcttgtccg agacttcacg cgcgaagtcg
     8161 tcagctttca ggacttgtgc ggaggtccat ggatcggagg actgcttttc gtctccacgg
     8221 attgccggaa cggagacgag cgtcgctgat acagcgaaca aaacggtaag tgcagaaacc
     8281 aaaaaaagag tttggaaacg gcttgttgct ggcgccaaag gagtcctccg agaaatgccc
     8341 cgattctgga ccgaatggat ttgtagattc aaagaaatga ccgcctattt tttcagttcc
     8401 gcgcgcgtgc gctggagctt cgtggccgtg gtgcggagag tttcgggaac gtttttgttg
     8461 gaggtcagac gcttgaggtc gacgacgttg agcatcggaa gcatgtgcat ggtgacgtcg
     8521 agtggcgtct tgggattggt gatgaggttg cggacgacgc tgtagttttt gcggaaattg
     8581 cggttgccgg cgatgaggcg gagaacctca tcggtgaggc tggacatcgc agcgaaagct
     8641 tcgacttcct ggtcggttag gcggggagac tgaagaaccg cacgctggac aactttgttg
     8701 ttgtcgcgaa tgagcgtgcg gcgcgcttcc gggccgccct tcatcgcgaa ttgcacgcgc
     8761 tgcgcgacgg tcattttcgc caggcgctga atcagagtct ggcgcctggc gacgtcggat
     8821 tccgccgcag ctgcggcttc ggcgagggcg gcttcgtcga tgacattgtc tgggccgtga
     8881 agttcgtgga gctgactttt ttgttcgacg gtcaccgagg aagaatgctc gagagcggag
     8941 gccagcgcgg gagattcgct gatgcggtcg agttcttcca tcagcgcttg gatgccgaga
     9001 gtcgagaggt ggcgaacgag aggaacgaga tgttcggcag aacagttctt gttttgcacc
     9061 atggcgtcgc agacgccggg cttatcggca agattctttg ccgcgtaaga aaagagcgcg
     9121 gggagagcat gctcgcgttt gagggcttcg acaaaggatt ccacggactg cgagagaatc
     9181 gcatcctgcg cgcgggctga gaccatttcg tcggcgtcat gggcaagaac agcgagaatc
     9241 tccgcgtgat ccgcgggtgc gagatgcgca ccgccggtgc aaacggcgat ttttcgttcg
     9301 cgcgtggcgc gcccgtggcg cagatcttcg agaacttcag ggctgagagt ttccactggg
     9361 ttatggcctc aagtccatct cactaaggac tattctaccg gaggcgccgg gcgccccgag
     9421 gggcggcatg gccttgccgt tcaattccag caacaccgcg gaggagtccc cggcggtcac
     9481 ctcgaaatgg tcattggcgg gaaagtgaag agtttccccg gcaggcaact cggcgtcgag
     9541 gagcagtttg ttgtccgcga ggatgcggac gcgcgtggcg gcggaggtgg agacggagag
     9601 gtcgagaaga gccgcggcgg aggccacaga agaaaatggt tccgctgatc ccggtgaagt
     9661 ggaaccgggg tgaggctgca tcggcaagga agcagcggcg gactttttag tggcgcgata
     9721 cgcggcgtag cggtgccagc cgtagcggcc cgcgtagaaa agaccggcga gcaaaagaag
     9781 caagaggaga acggccagag cgggaagcca tttcggcggg ggtggaatgc gttcctcggt
     9841 tttgggagga tgcgcggcca aatgttcggc acgggcgaga tcgtactcgc cgaggagtga
     9901 ttcttcattg aggcccagat aacgcgcgat ggtgcgcacg aagccgtggc cgaagacgcc
     9961 gccgggcagc ttcgcccaat cttcattctc gagggcttgg acaaaacggg tgctaatgcg
    10021 cgtggccttg gtgagttcgt ccatggaaac ttcgcgcatc tcgcgctcgc gcttcaggcg
    10081 ttcaccaaaa tttcccttgg ccatgaaccg gaacctgtcc ttgacaatcc cgttgcaacc
    10141 ccgaagtggc cgataccaag cgcgaccgca gggcggttga ttgcaagtgc aaccgcaaag
    10201 cggttgacac ggcgggcgct cgcgaaaaaa cagcgtggga tccctgaacg gactataggc
    10261 ggaggaatcc aacgctgtca aacgagtcga gtcgagattc gacagcgtga tgctcctcag
    10321 ggcagattct ctgatctgga atgaggagag gtccctccgt cgccaagctc cctccccgac
    10381 gaggccgggg caagcgggac ggaagtctcc attgggaatg aggagagatc ccttccgaca
    10441 aggtcggaac cttcggtcga ctcccgatgg tccggacgga tcgggactcg caaacagcgc
    10501 tcgtcgcagg ggatagacat gcttcgtcgc atgtgaaggg agatccttcg tcgcgatgct
    10561 cctaaggaca ggaatcctca tcggattccc ggttaacaat acccccctac gccccatgtt
    10621 tttcataagt gttcattcta aaggaagtta aagtcctttg ttttgataca cttttgcaag
    10681 tgttgattct aaagggactt acgttgcacc aaaattgtgc aaaatctgtc cttccgccat
    10741 gtttttcata agtgttcatt ctaaacggct taaagttctt tagaatgaac acttctataa
    10801 gtgttgattc taaataagtt acacggttga aggtacgcag gtttgaatgt tgggctgctc
    10861 gaagccggag gagcgcgagg aggcgcgagc ctgatggtgc ggcggtttgg agcttgacaa
    10921 acacgggaaa gaatagcagg aagtagcccg tttgtcaata tatttattga atatcattcg
    10981 aacgggccgt aagacgttga aatgtttgca ggttacaagt ttgaaggttg aagttcaggg
    11041 atggggatcc ggggcgggga agcggaaaat ccagagaaag gaataatgtg gaaagaagat
    11101 ggaggaagag tcaaaacccg cacccttggc ctaaagcgca aagggattga ttggataaga
    11161 gcgaaggctc cgttcggtag ccgggtatgc ttctggttgg aggaccgaag cactcggtag
    11221 gaaggagcct tcgatgatgc ttattggatg tgattatcac cccagttggc aacagatttg
    11281 ttggctggac acggccacgg gagaggccgg agaaaagaag ttggagcatg cgtcgggaga
    11341 agcggagaag ttttaccgtg gattgcctgg ggctgctctg attgggatgg agtccaccgg
    11401 gaactgtcag tggtttgtgg agatggctac gacgttggga cacgaagtgt tggtcggtga
    11461 tgcggcgaag attcgcgcca gcgaggtgcg gcagcagaaa cacgaccggc gcgacgcggc
    11521 gctgattttg aaactgttgc gggaaggacg gtttccgcaa atctggactc cgtcaggtga
    11581 agaaaaggat ctgcggcagc ttttgattca tcgctacaag ctggtgcgga ttcgcgcgca
    11641 ggtgaagacc gagctgcagc acctggcgat gaaccagggc atcaccaaga agcgcaagct
    11701 gtggagcaaa gcgggcgaga aagtgctccg cgagttgccg ctgaagccgt gggccagccg
    11761 gcggcgagaa gacctgttca gggtaagaga gatgttgaat gggcagattg atctgctcga
    11821 ccaagcggtg gtggaagccg cggagaagaa cgaaaaagcg cggctgctga tgacgcagcc
    11881 gggagtggga ccgattactt cgctagcgtt cgtgctgacg atgggtgacg tgaagcgttt
    11941 tcagcgcggg aagcaggtgg ccagttacct ggggctgatt ccgcgggagt acagttcggg
    12001 tggaaagcag cgcttgggtt cgatcagcaa gcaggggaat cggttcatga ggatgctgct
    12061 ggtcgaggcg gcgcaaggtg cggtgcggta cgatccggga tttcgcaatg agtatttgca
    12121 tcgctgccac acgaagccga aaggagtggc caaagtggcg gcggcaagaa agttggcagt
    12181 gcgactctac tggatgctgc gcacgaatac cgggtatcca gagatcgttc gtgtcgagag
    12241 cagctcgcgg gtgccgctgg tcagcgaaag ctagaccgag ggattgattg ggcgctctcg
    12301 tcccggtgca cgccgggatt cggatagaag aatcatggtc gcggtttagg ccgtatcgat
    12361 ggttggcgga acgatccgcc gaaagctgat tcaccgtgag agttt
//
LOCUS       DASYVO010000495         4750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_140232, whole genome shotgun sequence.
ACCESSION   DASYVO010000495 DASYVO010000000
VERSION     DASYVO010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140232"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..2737
                     /locus_tag="VGR03_17210"
     CDS             197..2737
                     /locus_tag="VGR03_17210"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEV1996075.1"
                     /translation="MPRPSNLSPDNILRFLQVRSEPASTSEITQGLHLKKSDTRPLFK
                     MLVKLKKRRAIEELPGGRYCLPGRKAEQKASGKGARPNESQHQRPSSLVSRDEITGRL
                     VLHHDGYGFVVPDSAVPQLDGDIFIPRDAVEDAMHGDHVLVRIQRTGGVSGAQRAEGR
                     IVRILGRAHATVVGLFRYGAQGNFVLPYDFRIQHEVEIPRGHELTPGLWKKLGFSGAD
                     EPSLRSRRIPRLEELDGAVVDVELIRYARGGASATGRIIEILGRPGDLGVDTEIIIRK
                     HHLPHSFSSEVLAEAEQRAKPVREVEHAGREDFRHLPIVTIDGETARDFDDAVYVERR
                     SDGGWLLQVHIADVAHYVRTGSPLDPEARLRGTSVYFPDRAVPMLPEALSSGVCSLKP
                     REDRLVMSALMEFEAAGHMKSSHMTPGIIRSAERMTYTNVNKVLEGDPEMAELYAALA
                     HHFRDMKELALLLNARRNEHGSIDFDLPEPVIEFDKEQRMTNITRSERNIAHRLIEEF
                     MLAANRAVAGYLLRRGIDSLHRVHEKPDVRKVLEFEELARAFGYSLGVEDLHQREIPV
                     RHGRVPAPAKAGRPDSYGHGRERGMMVVLSGSTELRITPQHYQRLIRKVVGTPVERII
                     SYLMLRSLKQARYAADSLGHFALGFDEYTHFTSPIRRYPDLIVHRILKWTLEHPKEVP
                     STSQGPSAAPRGAEAALYSHGNLEEIASEASEAERRANGAERELMDWKTAQFMEEHLG
                     EEYDALIISVQKYGCFVELFEVFVEGLLPVGALEEFANARCVYRERDHAIVALASQGG
                     RPGTAGARRGRGAKARPLEWHLGDKVRVRAERIDPMRRRVEFALVAEN"
     gene            complement(2756..3304)
                     /locus_tag="VGR03_17215"
     CDS             complement(2756..3304)
                     /locus_tag="VGR03_17215"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV1996076.1"
                     /translation="METQQERRKRTRQIGGERRADDVSRIANALKRRGRIALRVHGTS
                     MLPWVRPGDVGVIRQISIENVRCGDVVLFRRDNHLFVHRIVEKRGSQDTAQLFSKGDA
                     HPTSDGVVEKQELLGRVMRIYRNGQRIDLDAPGQLALGLFISQLSLHSRFWYPAARFA
                     VLVTRPARWVMHALHLSSPAVQ"
     gene            complement(3811..>4750)
                     /locus_tag="VGR03_17220"
     CDS             complement(3811..>4750)
                     /locus_tag="VGR03_17220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV1996077.1"
                     /translation="ILAAARSLHFDISLKTNALLITRERAARLREFRVRRVQISVYSD
                     IPEVHDAITKVPGSLQRTLAAIPLLIEQGLQVKLACPLMQENLMAYRGVMALAEKLRV
                     PYVLDLTITPMMDGSETTLAHRVSVSSLLPVLQDPALHACKPQPDSHLSSAPKTIPAV
                     TGSAVSSVIESSAYEDLPCSAGHNSCYISPYGDVFPCVQLPQAAGNLRHERFAEIWYH
                     APRLERLRAIRESHLPICSRCEIRSYCERCPGLALMEGGDLLGAYERACDLAEQKARL
                     AGLADPVSALHQERAASSQTTTGNKIAALVTDSSPD"
BASE COUNT          985 a         1516 c         1335 g          914 t
ORIGIN      
        1 gaggcgggcc aggtcgtccc gcctccctga tttcccctct cggggaatcc ggccagtctg
       61 acggagggca actagccggt cgtgtgttgg cctatatata attagcccgg aaaccaaaaa
      121 aaagttccgg agaaaccaag aaaagtgact aaaatcacgg gggaaaacta gcgcccgcac
      181 cccggcgcaa gaccgcatgc ctcgcccttc aaacctgagc ccggataaca tattgcgttt
      241 tctgcaggtt aggagcgagc ccgcctccac tagcgagatt acccagggcc ttcatctcaa
      301 gaaatccgac acccgccccc tgttcaagat gctcgtcaag ctgaaaaaac gccgtgctat
      361 cgaggaattg ccgggcggac ggtattgcct ccctggccga aaagcggaac agaaggcctc
      421 aggcaagggt gcgcgtccga acgaatcgca gcatcagaga ccttcttctc tcgtgagccg
      481 cgatgagatt acgggacgcc tggtcctcca tcacgacggc tatggctttg tcgttccaga
      541 ttcagccgtc ccgcaactcg atggcgacat cttcattccg cgagacgccg tcgaagacgc
      601 tatgcacggt gaccacgtcc tggtaagaat ccagcgcacc ggcggcgttt ccggtgcgca
      661 acgcgccgaa ggccgcatcg ttcgaatcct gggccgcgcg catgccaccg tcgtcggact
      721 gtttcgctac ggggcgcaag gtaatttcgt ccttccctat gattttcgca tccagcatga
      781 agtggagatc ccgcgtggcc atgagctgac ccctggcctc tggaaaaaac tgggcttcag
      841 cggcgcggat gaaccaagcc tacgatcgag acgcataccc cgcctggagg agcttgacgg
      901 tgcggtcgta gatgtcgagc taattcgcta tgcacgtggc ggcgcatcgg ccacgggccg
      961 catcatcgaa atacttgggc ggccgggaga cctcggcgtc gataccgaaa tcatcattcg
     1021 gaaacatcac ctcccgcatt ccttctcctc cgaagtcctg gcagaggcgg agcagcgcgc
     1081 aaaaccggtg agggaagtcg agcacgcagg ccgcgaagat tttcgccact tgccgatcgt
     1141 aaccatcgac ggtgaaaccg cacgcgattt cgacgacgcc gtttacgtcg agcgccgctc
     1201 cgacggtggc tggctcctcc aggtgcacat cgccgacgtc gctcattacg ttcgaaccgg
     1261 cagccccctc gacccggaag cccgtcttcg cggcacctct gtgtactttc ccgatcgcgc
     1321 cgtgccgatg cttcccgaag ctctttcgag cggcgtgtgc tccctgaaac ctcgcgagga
     1381 ccgcctggtc atgagcgcgc tgatggaatt cgaagctgcc gggcacatga aatcctccca
     1441 catgactccc ggcatcatcc gctccgccga gcgcatgacc tataccaacg tcaacaaagt
     1501 cctcgaaggc gaccccgaga tggccgagct ttatgccgcg ctcgcgcatc atttccggga
     1561 tatgaaagag ctggcgctgc tcctgaatgc gcgccgcaac gagcacggct ccatcgattt
     1621 cgatctcccc gagccggtca tcgaattcga caaagaacag cgcatgacca acatcacgcg
     1681 cagcgaaaga aacatcgccc accgcctcat cgaagagttc atgctcgccg ccaaccgtgc
     1741 cgtggccggc tatcttctgc ggcgcggcat cgattccctg caccgcgtcc acgagaagcc
     1801 cgacgtccgc aaggtcctgg agttcgaaga gctggcgcgc gccttcggct attccctggg
     1861 ggtcgaagat cttcaccaac gcgaaatccc ggtgcggcat ggccgcgttc cggcgcccgc
     1921 gaaagcgggc cggcccgatt cctatggcca tggccgcgag cgcggcatga tggtcgtcct
     1981 ctccggctcc actgaactgc gcattacgcc gcagcattac cagcggctga ttcggaaggt
     2041 cgtaggaacg cccgtggagc gcatcatttc ctacctcatg ctccgctcgc tcaagcaggc
     2101 ccgctacgcc gcggactcgc tcggccattt cgccctcggc tttgacgagt acacacattt
     2161 tacttcaccc atccgccgct accccgatct catcgtccat cgcattttga aatggaccct
     2221 tgagcatccg aaggaggttc cgtcaacttc gcaggggccc tccgcagcgc cgcgaggcgc
     2281 cgaggctgcg ctctattcgc atggaaacct tgaggaaatc gcttcggaag cctccgaagc
     2341 cgagcgccgc gccaacggag ctgagcgcga gctcatggac tggaaaaccg cgcagttcat
     2401 ggaagagcat ctcggcgagg aatacgacgc tcttatcatt tccgttcaga aatacggatg
     2461 tttcgtcgag ctcttcgagg tgttcgtgga aggcctgctc cccgtaggcg cactggaaga
     2521 attcgccaac gcccgctgcg tctatcgcga gcgcgatcac gccatcgtcg ctctcgcgag
     2581 tcaaggcggc cggcccggaa cagccggcgc gcggcggggg cgcggtgcaa aggcccggcc
     2641 actcgagtgg cacctcggcg ataaagtacg cgtacgcgcc gaacgcatcg atcccatgcg
     2701 aagacgcgtc gaatttgccc tggtcgctga gaattgaaga aggaatttca ggaattcact
     2761 gaactgccgg gctggataga tgcagtgcgt gcatcaccca ccgtgccggc cgggtcacca
     2821 acaccgcaaa tcgcgcggcc ggataccaaa accggctgtg cagcgacagt tgcgaaatga
     2881 acaatcccag ggcgagctgg cccggcgcat cgagatcaat ccgctggcca ttccggtaaa
     2941 ttcgcattac gcggcccaat aattcctgtt tttcaacgac accgtcggac gtcggatgcg
     3001 catcgccttt ggaaaaaagc tgcgcggtat cctgcgaacc ccgcttttcc acgatgcggt
     3061 gcacgaaaag atgattgtcg cgccggaaca ggacaacgtc gccgcagcgc acgttctcga
     3121 tggagatctg tcgaatcacc ccgacatctc cgggccgcac ccagggaagc atgcttgtcc
     3181 catgtacgcg cagcgcgatg cggccgcggc gcttcagcgc attcgcaatg cgcgaaacat
     3241 cgtccgcacg gcgttcaccg ccgatctgac gcgttctctt ccgccgctct tgctgagtct
     3301 ccatcaaatc ctcaaagttg aaatcccaac gtccacgtga ctccgcttca tttccgcaga
     3361 aaggtcgcgc gtcaccctaa cggacgcgaa cacaggggca cgtgttggag tcaaatcgca
     3421 catacccgaa gcctgtcccg aagaggctca agaaaacgag aaatcgttcg gaaacaaaac
     3481 gggtgcaact tctttctccc aggctgacgt ttcaggtgcg aatggtcaat gggagagaag
     3541 ttccagtttg cctaaagcta cagtactcaa ttggagcagc ttacgggaga ctctgccgtc
     3601 cgctaaggga gcaagcgagc ggcgctcatt tacgaccttt taggagaagc gttacaaatg
     3661 cccccctggc ccggcaattt gccgcacgcc agcgccagag tttcgtagat gggttccgaa
     3721 accaccgtgg gctttgcgta cggcttctta gccccggttg ctccgggagt caggggagaa
     3781 gtcttgtgat cttcgggcat tcgatgttcc ttagtctgga ctagagtctg tcacaagggc
     3841 agcaattttg ttcccagtgg ttgtttggct cgatgccgct cgttcttgat gaagtgcgct
     3901 taccgggtcg gcgagacccg ccagccgcgc cttttgttcc gcaagatcgc aagcccgctc
     3961 gtaagccccc aacagatctc cgccttccat cagcgccaat cccgggcagc gctcgcagta
     4021 acttcgtatc tcgcatctcg agcagattgg cagatgactc tctctgatcg cgcgtaaccg
     4081 ttcgagccgc ggcgcgtgat accagatttc ggcgaatcgc tcgtgccgca aattccctgc
     4141 cgcctgtggc aactgcacgc acgggaacac gtctccataa ggcgaaatgt aacaactgtt
     4201 gtgccccgcg ctgcacggca ggtcttcgta agccgagctt tcgatcacgc tcgaaactgc
     4261 gcttccagtc accgctggaa tagttttcgg tgcgctggat aagtgggaat ccggctgggg
     4321 cttgcaagcg tgcaacgcgg gatcctgcaa caccggcaga agcgaggata cggaaacccg
     4381 gtgcgccagc gtcgtttccg agccgtccat catcggcgta attgtcaggt ccaggacgta
     4441 aggaacacgc agtttttccg cgagtgccat cacaccgcgg tacgccatca gattttcctg
     4501 catcagcgga caagccagct ttacctgcaa tccttgttcg atcagcagcg gaatcgccgc
     4561 cagcgtccgc tgcagcgacc ccggcacctt ggtgatggcg tcgtgtacct ccggaatatc
     4621 gctgtagacg ctgatctgca cgcgccgcac gcgaaattcg cgcagccgtg ccgctcgttc
     4681 gcgcgtgatc agcagcgcgt tggtcttcaa gctgatgtcg aaatgcaagc tcctggccgc
     4741 tgccagaatc
//
LOCUS       DASYVO010000496         2549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_183269, whole genome shotgun sequence.
ACCESSION   DASYVO010000496 DASYVO010000000
VERSION     DASYVO010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183269"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VGR03_17225"
     CDS             <1..1073
                     /locus_tag="VGR03_17225"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEV1996078.1"
                     /translation="LRDEIGLYDAMLMFYHGLVAFDHVQHRLWIVRNVFTEGDGSLRA
                     KYNAAVREIKETRRLLDEPVAAERPKKPSKAKKRGPLKVTSNFRRPEYLAAVRKAKQY
                     IRAGDIFQVVLSQRFSAKTQAEPFEIYRELRALNPSPYLFYLQLNDVSVVGSSPEMLV
                     KVQGRDVFYRPIAGTRWRGKDEAEDQRLEREMLASEKERAEHIMLVDLGRNDLGRVCD
                     YGTVTPEKLLTVERYSHVMHLVSSLRGRLREDVDCFDALMACFPAGTVSGAPKVRAME
                     IIEELERTRRGIYAGGILYLDFAGNLDSCIALRTMVVKNGVAHVQAGGGIVADSTPEG
                     EYQESVNKSKALLAALEKAHRR"
     gene            1190..1753
                     /locus_tag="VGR03_17230"
     CDS             1190..1753
                     /locus_tag="VGR03_17230"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV1996079.1"
                     /translation="MILLLDNYDSFTYNLAQYLGELGCTVEVHRNDKITVEEIVRRKP
                     ERIVISPGPCTPQEAGISVELVERLAGKFPILGVCLGHQAIGAAFGGKIVRAPKLFHG
                     KTSQIHHDGRSIFRKLPDPFTATRYHSLIVEKKSLPRELMITAETDDGIIMGLRHRRH
                     KIEGVQFHPESVLTESGKQLLKNFLSL"
     gene            complement(1806..>2549)
                     /locus_tag="VGR03_17235"
     CDS             complement(1806..>2549)
                     /locus_tag="VGR03_17235"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HEV1996080.1"
                     /translation="NWAPNPAMELSRLLASMKDTDGRVLIDGYYDDVTPLGEVEKKAL
                     AEMPENDADLERELGIAKPEGGGKKLVELIMQPSLNIRGLRSAYVGGQAQNVVPDRAE
                     ASLEARLVKGEDPKKKFEQIAAFIEKQGFFVIDHEPTMEERRAHARIAKVIYEGGYRA
                     SRTLMDLPMSKAIVEAVRGAAGNSTVVMPSSGGSVPMYIFEDLGLQWVGVPIVNYDNR
                     QHSSDENLRMGHFWRGMEIYGVILADLNW"
BASE COUNT          536 a          826 c          664 g          523 t
ORIGIN      
        1 gcctccgcga tgaaatcggc ctctatgacg cgatgctgat gttctatcat ggccttgtcg
       61 cattcgatca cgtgcagcac cgcttgtgga tcgttcgcaa tgttttcacc gaaggggacg
      121 gtagccttcg cgcgaagtac aacgccgccg tgcgcgaaat caaagagacg cgccggctgc
      181 tcgatgagcc ggtggctgcg gaaaggccga agaagccatc caaagcaaag aaacgcgggc
      241 cgctgaaagt gacttcaaat ttccgccggc ccgaatacct ggctgccgtg cgcaaagcga
      301 agcagtacat tcgtgccggg gatatttttc aggtcgtgct gagccagcgg ttctcggcaa
      361 agacgcaggc cgagccattt gaaatctatc gcgagctgcg cgcgctgaat ccttcgcctt
      421 atcttttcta cctgcagctg aacgacgtct ccgttgtcgg cagctcgccg gaaatgctcg
      481 tcaaagttca gggacgcgat gttttctacc gtcccatcgc cggcacgcgt tggcgcggca
      541 aggacgaagc cgaagaccag cgcctcgaac gcgaaatgct ggccagcgaa aaagagcgcg
      601 ccgagcacat catgctcgtc gatctcggac gcaacgatct tggccgcgtc tgcgactacg
      661 gcacggtgac tcccgaaaag ttgctcaccg tcgagcgcta ctcgcacgtc atgcacctgg
      721 tttccagcct gcgtggccgc ttgcgcgaag acgtggattg tttcgacgcg ctgatggcct
      781 gttttcccgc cggcacggtg tccggcgcgc caaaagttcg cgccatggaa atcatcgagg
      841 agctggagcg cacccgccgc ggaatttatg ccggcggcat tctctacctc gattttgccg
      901 gcaatctcga ttcctgcatc gcgcttcgca ccatggtggt gaaaaacggc gtcgcccacg
      961 tccaagccgg cggcggcatt gttgcggatt ccacgcccga gggcgaatat caggagagcg
     1021 tcaataagtc gaaagcgttg ctcgcagcgc tggaaaaagc gcaccggcgc tagtgggaca
     1081 gactttagtc tgtccgcctg aacctaggcc aaattgatac gccgagctaa ggcttgacgg
     1141 cactaatgtc ggaagaacgg tattagaatt aggagacaag tactgcgtca tgattctgct
     1201 tctcgacaac tacgattcct ttacctacaa cctcgcgcag tatttaggcg aactgggttg
     1261 caccgtcgaa gttcatcgca acgacaagat taccgtggaa gagattgtcc gccgaaagcc
     1321 ggaacggatc gtaatctctc ccggtccttg cacgccgcag gaggcgggca tttccgtaga
     1381 gctggtcgag cgtctggccg gaaaattccc catcctcggt gtgtgcctcg gccatcaagc
     1441 catcggcgct gcgttcggcg ggaaaatcgt ccgtgctccg aaactctttc acggcaagac
     1501 aagccagatc caccacgacg gcaggagtat tttccgcaag ctgccggatc ccttcactgc
     1561 cacgcgctac cattccctga tcgtcgagaa aaaatccttg ccgcgcgaat tgatgatcac
     1621 cgccgaaacc gacgacggca tcatcatggg cttgcgccat cgccgccaca aaatcgaagg
     1681 cgtccagttc catcccgaat ccgtcctcac cgaatccggc aagcagctgc tcaagaattt
     1741 tctttctttg taggggcaca gcatgctgcg ccccagcttg gctgaacgct tcagatctcg
     1801 agccctcacc agttcaaatc cgccaaaatc accccgtaaa tctccatccc tcgccaaaaa
     1861 tgtcccatcc gcaaattttc atccgagctg tgctggcgat tgtcgtaatt cacgatcggc
     1921 acgcccaccc actgcaaccc caggtcctcg aagatataca tcggcacgct gcccccagac
     1981 gacggcataa ccaccgtgct gttgcccgcc gcccctcgca ccgcttcgac gatcgccttg
     2041 gacatcggca aatccatcag cgtgcgcgac gcccgatacc cgccctcata aatgactttc
     2101 gcgatccgtg catgcgcgcg gcgttcctcc atggttggct cgtggtcgat cacgaaaaat
     2161 ccctgcttct cgataaacgc cgcgatctgc tcaaacttct tcttcggatc ttcccccttc
     2221 accagccgcg cctcaagcga agcctccgcc cgatcgggca ccacattctg cgcctgccca
     2281 cccacatacg cgctgcgcaa tccgcgaata ttcagcgagg gctgcatgat caattcgacc
     2341 agcttttttc cgccaccctc cggtttggcg atgcccagct cgcgttcgag atcggcgtcg
     2401 ttctccggca tctctgcgag cgcctttttc tccacctcgc ccagcggtgt gacatcgtcg
     2461 taatagccat caatcagcac ccggccatcg gtatccttca tcgatgccag cagccgcgac
     2521 aactccatcg ccggattcgg cgcccaatt
//
LOCUS       DASYVO010000497         1857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_183271, whole genome shotgun sequence.
ACCESSION   DASYVO010000497 DASYVO010000000
VERSION     DASYVO010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183271"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VGR03_17240"
     CDS             <1..305
                     /locus_tag="VGR03_17240"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1996081.1"
                     /translation="VLAADPSSPAAHRGLAEIDRRQGKMDDAVKELHASLEARDSAVV
                     RTMLAKIYLEQKKLDLARAEVEKALKLAPNYAEAKQLLEHLQNSKPSGKKPGGGAQ"
     gene            302..1702
                     /locus_tag="VGR03_17245"
     CDS             302..1702
                     /locus_tag="VGR03_17245"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEV1996082.1"
                     /translation="MSGWFHKMKRSFHAKRSEFVAASFFFCVLCAGALFFLMPAVAAD
                     EHSSNPLVLELRLDGVVDPILATYIDEGLADAASRHAALVLITMNTPGGLSVSMQDMI
                     QHILVSPVPVAVYVSPTGGRGASAGFYILLSADIAAMAPGTHTGSATPVMAIGGILPM
                     QIDDAFRKKINNDATAFLRSFTERRGRNPALAEKAITEGSAFTEKEALDGKMIDLIVN
                     SPDDLLRQLDGRAITRFDGTQAKLALKNAARTPFELSARQKFLARIVQPDVFFVLLIL
                     GVLGVYTEFTHPGAIAPGVIGGICLILALYAMNFLPINLAGLFLILLALAFFILEAKA
                     PSHGVLAFGGVVSMFLGAIFLISSPLTPGGVSAGVALAGTVPFAILAVVLMRLVLRSR
                     RWKSATGKEELIGSQGVAVARLSAGAEGMVRVHGELWRAESSRPVQEGETVRILRVEG
                     LKLYVEPAEVAVSAAK"
BASE COUNT          346 a          505 c          592 g          414 t
ORIGIN      
        1 ctgttcttgc cgccgatccc agcagccccg cggcacaccg cgggctcgcg gaaattgacc
       61 gccgccaggg caaaatggat gatgcggtga aggaattgca tgcgtcgctg gaagcacgcg
      121 attccgctgt ggttcgaacg atgctcgcga aaatctacct cgagcagaag aaactggatc
      181 tcgcgcgagc ggaagtcgag aaagcactga agctggcgcc gaactatgct gaggccaagc
      241 aactgctcga acatttgcag aattccaaac caagcgggaa gaaaccaggt ggaggtgcgc
      301 aatgagcggt tggttccata agatgaagcg ctcctttcat gccaagcgct ccgaatttgt
      361 tgcggcctcc ttctttttct gcgtgctgtg cgccggcgca cttttcttcc tgatgcctgc
      421 ggtcgcggcg gacgagcact cgtccaaccc cctcgttctt gaactgaggc tcgatggcgt
      481 agtcgatccc atcctggcca cgtatattga tgaaggtctc gccgacgcgg cgagccgtca
      541 cgcggcgctt gttttgatca ccatgaacac accgggcggg ctcagtgtgt cgatgcaaga
      601 catgatccag cacatcctcg tgtccccggt gccggtcgcc gtgtacgttt cgcctacggg
      661 tgggcgagga gcctcggcgg gcttctacat tcttctctcc gctgacatcg ccgcgatggc
      721 gccggggaca catacgggct cggctacacc ggtcatggcg atcggcggta ttctgcccat
      781 gcagattgat gatgcatttc gaaagaagat caataacgac gccacggcgt ttctgaggag
      841 tttcacggag agacgcggac gcaacccggc gctggcggag aaggccatca cggaaggcag
      901 tgcgttcacg gagaaggaag ccctggacgg gaaaatgatc gacttgatcg tgaattcgcc
      961 cgatgatctc ttgcggcaac tggacggccg ggcgatcacg cgattcgacg gcacccaggc
     1021 gaagctcgcg ctgaagaatg cggcgcgcac gccttttgaa ctttcggccc ggcagaaatt
     1081 cctcgcgcgc attgttcagc ccgatgtttt ctttgtgctg ttgattcttg gcgttcttgg
     1141 cgtctacacc gagttcacgc accccggagc gatcgctccc ggagtcatag gcggtatttg
     1201 cctgattctc gcgctgtacg ccatgaattt tctgcccatc aatcttgcgg gcctgttcct
     1261 gattttgctg gctcttgcgt ttttcatact cgaagcgaaa gctccgagtc atggcgtact
     1321 ggctttcggc ggagtagtgt cgatgtttct gggcgcgatc ttcctgatta gctcgccgct
     1381 cactcctgga ggagtgagcg caggcgtggc cctggccggg acggtgccgt tcgcgattct
     1441 ggccgtggtt ctgatgcggc tggtgttgcg ttcgcggcga tggaaatcgg caacgggaaa
     1501 ggaagagctc attggatcgc agggcgtcgc ggttgcgcgg ctgtccgcag gcgcggaggg
     1561 catggttcgc gtgcatggcg agctgtggcg agcggaatct tcgcggcctg tgcaggaagg
     1621 cgagaccgtc cggattttgc gtgtcgaagg actgaagctg tacgttgagc cggccgaggt
     1681 ggctgtttca gcggcaaaat aggaattttc acgttcagga ggaaaacatg gaacctggtc
     1741 ttattgggat agcgttgatc gtcggcctcg ggctcatctg gttctggaat tcgatctata
     1801 tcattaagga gtgggaacgc ggcgtggtcc ttcggctggg gcgcttgata cccgagg
//
LOCUS       DASYVO010000498         3655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_140315, whole genome shotgun sequence.
ACCESSION   DASYVO010000498 DASYVO010000000
VERSION     DASYVO010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140315"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VGR03_17250"
     CDS             complement(<1..931)
                     /locus_tag="VGR03_17250"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEV1996083.1"
                     /translation="MPIQKTEKIWHNGHWINWDDATLHVASHVISYGSAVFEGIRCYD
                     TKQGAAIFRLRDHMQRLINSAKIYRMELPHSLDEFCNVATELVRRNKLNACYIKPIVL
                     RGYGEAGVNPLTSPIECYMACWPWGAYLGPDAQTRGVDVCVSSWTRIAPNTLPAMSKA
                     AANYMNSQLIRMEASFNGFAEGIALDASGYLSEGSGMNIFLVHEGTVSTPPLAASVLP
                     GITRASVITLCEDMRIPVKEQVIPREMLYIADEVFFAGTAVEITPIRSVDRIPVAKGE
                     PGAITKRLQGEFFAITSGTKPDRHHWLTPCNAPV"
     gene            complement(1113..1994)
                     /locus_tag="VGR03_17255"
     CDS             complement(1113..1994)
                     /locus_tag="VGR03_17255"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1996084.1"
                     /translation="MKHFGILTASRMSLAMRNKAFLFFSLVMPLAFFFLFAGIFGKGQ
                     PEKVAYLMGPVIGLTVMGGFWGLSASLVMYREQGILRRYRLAPLTASDMLASSIAANY
                     ILTLPTVAVEIILARLLFHVTHMGDLLSLAILVTVATIAFAALGLVVASVTNTMQETQ
                     VICQIIWFALLFFSGATFPLPILPRIVQRFSVFLPATYLVTGLQKAMVQSKPFWQLSV
                     ELVALAAWAVLAFFVAAKLFRWETEEKLPRNAKLWAVATIVPFLILGAWESKHGTILA
                     QSKTIYQSLDAPSPPQK"
     gene            complement(1991..2911)
                     /locus_tag="VGR03_17260"
     CDS             complement(1991..2911)
                     /locus_tag="VGR03_17260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1996085.1"
                     /translation="MSNTALQVENLTKRYGDVEALRGVSFAVEEGEVFGLLGPNGAGK
                     TTTVEILEGLRRRDGGSVSVCGMDPQRDPQKLKSEIGAALQATALPEKMRVIEALRLF
                     AHFYKRRRNPEELLKRFGLEEKRNAFYNQLSGGQKQRLALAMALVNDPRVLFFDEPTA
                     GLDPQVRREIYDIIEELRREKKTIVLTTHYIEEAERLCDRVAIVDHGKIIALGTPREL
                     KQRSGNTTRLEVRLAKPAVVATLKGLDGVADCRSFDGTYILHCQRPPQAIVALVKHLE
                     AEGNELVSLEISTPSLEDVFIELTGKRLRD"
     gene            complement(2984..3337)
                     /locus_tag="VGR03_17265"
     CDS             complement(2984..3337)
                     /locus_tag="VGR03_17265"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEV1996086.1"
                     /translation="MSWAPVYELVKQIPRGRVLTYGVIAKYLRLRGGARSVGHAMAAT
                     PRGQGIPWHRVVGERGRILIREPYASLQRKLLETEGVAVLESCVSLKRCLWAPAVPRR
                     KALVKRKLHRLRKSR"
BASE COUNT          729 a         1001 c         1229 g          696 t
ORIGIN      
        1 tgacgggggc gttgcatggc gtgagccagt ggtggcggtc tggcttcgtg ccggaggtga
       61 tggcgaagaa ctccccctgg aggcgcttgg tgatggcgcc gggctcaccc ttggcgaccg
      121 ggatgcggtc cacggagcgg atcggcgtga tctcgacggc ggtgcccgca aagaaaactt
      181 catccgcgat gtacagcatc tcgcgcggga tgacctgctc tttcaccgga atgcgcatgt
      241 cttcgcacag ggtgatgacc gatgcgcgcg tgattccggg gaggacgctg gcggcgagcg
      301 gcggcgtaga gacggtaccc tcatgcacta gaaagatgtt catgccggag ccttcgctga
      361 ggtaaccgga ggcgtcgagg gcgattcctt cggcgaagcc gttgaaggag gcttccatcc
      421 ggatgagctg ggagttcatg taattcgcgg cggctttgga catggcgggg agcgtgttgg
      481 gagcgatgcg ggtccaggag ctgacgcaga catccacgcc tctggtctga gcatcgggtc
      541 cgagataagc gccccacggc cagcaggcca tgtagcactc aatcggggaa gtgagagggt
      601 tgactccggc ctcgccgtat ccgcgaagca caatcggctt gatgtagcag gcgttgagct
      661 tgttgcggcg caccaactcg gtggcgacgt tgcagaattc gtcgagggag tggggaagct
      721 ccatccggta gattttcgcg gagttgatga ggcgctgcat gtgatcgcgg agccggaaga
      781 tggcggcacc ctgcttcgtg tcgtagcagc ggatgccttc gaaaacggcg gagccgtagc
      841 tgattacgtg cgaagcgacg tgcagcgtgg catcgtccca gttgatccaa tgcccgttgt
      901 gccagatctt ctcggttttc tgaatcggca tgtcagctcc cggccggcgg tttgcggcct
      961 catgggccca cgcggcagga tacggcagcc gcggtgtccg gtcaatgcgt tgcgagtatc
     1021 aacgagggtg caggcctcag ccaatatggg cgggcgagac gcagccccaa aatcgtgtac
     1081 ggcctaaatc atccaggact cagtgccatg agctatttct gcgggggcga cggggcgtca
     1141 agcgattgat aaatggtctt cgattgcgcc aggatcgtgc cgtgcttgct ttcccacgcc
     1201 ccgagaatca ggaagggaac aatcgtggcc acggcccaaa gcttcgcgtt tcgcggaagc
     1261 ttttcttccg tctcccagcg gaagagcttc gcggcaacga aaaatgccag caccgcccag
     1321 gcggccaggg cgacgagctc aaccgacaat tgccagaatg gtttggactg caccatggcc
     1381 ttctgcaggc cggtgaccag ataggtcgcc ggcaggaaga ccgagaagcg ttgcacgatc
     1441 cggggaagaa tcggaagcgg aaacgttgcg ccggagaaga aaagcagcgc gaaccagatg
     1501 atctggcaga tgacctgggt ctcctgcatg gtgttcgtta cgctggcgac caccaggccg
     1561 agcgcggcga aggcaatcgt ggccactgtg acgaggatgg cgagcgagag caggtcaccc
     1621 atgtgcgtaa catgaaagag caagcgcgcg agaatgatct cgacggccac ggtaggcagt
     1681 gtgaggatgt agttcgcggc gatgctcgag gcgagcatgt ccgatgccgt gagcggggcc
     1741 aggcgatagc ggcggagaat tccctgctcg cggtacatca ccagcgaggc cgagagtccc
     1801 cagaagccgc ccatcacggt gagcccgatc accgggccca tcaagtaagc caccttctcc
     1861 ggctgaccct tgccgaatat tccggcgaag agaaagaaaa aagccagagg catgacgagg
     1921 ctgaagaaca agaaggcttt gttgcgcatg gccaggctca tgcggctggc cgtgagtatg
     1981 ccgaagtgtt tcaatcccgg agcctctttc cggtcagctc gatgaagaca tcctcgagag
     2041 atggcgtgga aatctccagg ctgaccagtt cgttgccttc cgcttcgagg tgcttgacca
     2101 gcgcgacgat ggcctgcggc gggcgctggc aatggaggat gtaggttccg tcgaaggagc
     2161 ggcagtcggc cacgccgtcg aggcctttca gggttgctac cacagcgggc ttcgcgaggc
     2221 gcacttcgag gcgcgtggtg ttgccggagc gctgcttgag ctcgcgcggg gtgccgagag
     2281 cgatgatctt gccgtgatcc acgatggcca cgcggtcgca caggcgctcg gcctcttcga
     2341 tgtaatgagt ggtgaggacg atggttttct tctcgcggcg cagttcttcg atgatgtcgt
     2401 agatttcgcg gcgcacttgc ggatcgagcc ccgctgtggg ctcgtcgaaa aacagcacgc
     2461 gcggatcgtt gaccagagcc atcgccaggg ccaggcgctg cttctgcccg cccgatagct
     2521 ggttgtagaa cgcattgcgc ttttcctcga ggccgaagcg cttgaggagc tcttcgggat
     2581 tgcggcggcg cttgtagaaa tgcgcaaaaa ggcggagcgc ttcgatgacg cgcatttttt
     2641 cagggagagc cgtggcctgc agcgcagccc caatttcgct ttttagcttt tgcgggtcgc
     2701 gctgcggatc catgccgcag acggaaacac ttccgccatc ccgccgtcga agtccttcga
     2761 ggatctctac cgtggtggtc ttgcctgcgc cgttcgggcc gagcaggccg aagacttcgc
     2821 cttcttcgac ggcgaagctc acgccgcgaa gcgcttccac gtcgccgtaa cgcttggtca
     2881 gattttcgac ttgcagggcg gtattggaca tggccgaagc gttcctagct taccgcagat
     2941 tgcatcttat gcccgaacgc tgcgggggga caacagatcc gcttcacctg cttttacgca
     3001 ggcgatgtaa ctttcgcttt acgagggcct ttcttcgcgg aacggcgggt gcccagaggc
     3061 agcgtttgag gcttacgcag gattcgagga ccgcgacacc ttcggtttcg aggagttttc
     3121 tctgtaggga agcgtagggc tcgcggatca gaatgcgccc gcgctcgcct accacgcgat
     3181 gccaggggat tccctggccc cggggagttg cggccatggc gtgccccacg ctacgggcgc
     3241 caccgcggag gcgcaggtat tttgcaatca ccccataggt taaaacacgg ccgcggggaa
     3301 tttgtttcac cagctcgtag acgggcgccc aagacattgc gaatcaaccg gatcgaggga
     3361 agaaagcgag atggtacgta gttcgtggcg cgcacacaag aataaaaatt gcccgaggag
     3421 ggtttaatct agcgtaaggt ccagccgctg atttcggaag cggcggggaa ggaaaggcat
     3481 cggaagaagc gaaagtgagc gagcgatgtg cacgaagggc cagttgacgg atacgagaca
     3541 accccgggcg gcgggccgca tcgaacttga cgagtgtagt ggaatatgaa tacactacga
     3601 acagtttcgg gattccgagg tgcaccgatg gcgtttgttc gccgcaaagg aaatg
//
LOCUS       DASYVO010000499         4934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_236655, whole genome shotgun sequence.
ACCESSION   DASYVO010000499 DASYVO010000000
VERSION     DASYVO010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236655"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1019
                     /gene="mtnP"
                     /locus_tag="VGR03_17270"
     CDS             114..1019
                     /gene="mtnP"
                     /locus_tag="VGR03_17270"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEV1996087.1"
                     /translation="MAAKKANKSKRAKSNATAAIGIIGGSGLYSMGGLTNTREIRVRT
                     PFGDPSDAIVLGTLEGKRVAFLARHGRGHRILPSEINYRANVYAMKLLGVERIISVSA
                     VGSLKEDLRPGEFLVPDQFFDRTKNRVSTFFGEGIVAHVAFAHPTCGQVSGVLADACV
                     HEAIKVHRRGTYICIEGPQFSTLAEAEVHRQLGFEVIGMTNVTEARLAREAEICYATI
                     AMITDYDCWHPEHESVTASQIIATLSQNAENAQKALRTAVRELPTSRSCKCESALEHA
                     LVTDLKIVPKATKKRLAAIIGKYIS"
     gene            1029..1937
                     /locus_tag="VGR03_17275"
     CDS             1029..1937
                     /locus_tag="VGR03_17275"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEV1996088.1"
                     /translation="MSLLVVGSVAFDALESPYGKVDRALGGAATYFAVAASFFTHVNL
                     VGIVGDDFTEVDAQIFHGRTIDIEGLERATGKTFFWAGRYSQNLNERVTLATELNVFS
                     EFKPRLPEKYRSAKYVFLANIAPDLQRDVLHQVKKRPKIAALDTMNYWIERSNAELRE
                     TLKHVDILMINDSETRELSSEHNLLRAAKHIFKMGPATLVVKRGEYGAMMVDKRGVFC
                     VPAFPLEEPHDPTGAGDSFAGGFMGYLAGCGDKSDASLRRAMVYGSVLGSFAVEQFGL
                     DRLRHLKRSEIHARARHFSKLTQFKL"
     gene            2004..2642
                     /locus_tag="VGR03_17280"
     CDS             2004..2642
                     /locus_tag="VGR03_17280"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV1996089.1"
                     /translation="MRLTVAAALGAVIGIERDLRRRPAGVRTSLFVCLATALFTILSG
                     EIARRLGDTGSTRIASNIVQGIGFLGAGAILREAGGVIGMTTAATVFVEAAIGMAAGG
                     GLYSVAVYATGLVLFGLICIGWAARRFNLKYRMMVFRITTGHADSVATEVQRLMAGMK
                     IPLQHFRISMAGANSIVEFESDVSHSQQETILTQLNRQGVITEVIPLEGHHE"
     gene            2639..3586
                     /locus_tag="VGR03_17285"
     CDS             2639..3586
                     /locus_tag="VGR03_17285"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEV1996090.1"
                     /translation="MTGSNGTPRKPKALAAGSRLGVFAPASPAESVDMIAGLAELKRH
                     GFQVVANQDTKAEGYFAGPPLERTNGFLGTLNSDQVDGLVALRGGYGSNYLLEFELEK
                     SLANLKCVIGFSDLTTLQIFLWQRCNWVTFYGPMVAAGLNAGPGARKGYDESSFLQAV
                     GRTEGGWKLRLRGEAVLAGQSLGRVLGGCMTLLEATIGTPWELETKDSILVLEDRAMK
                     PYQVDRVLMHLKQAGKFEGVRGIVLGDFPESEPAAAGGPTVREVCARILRPLGVPIVF
                     GAPVGHTVRPMLTIPLGIKARLDADGEGTLEFLESAVVA"
     gene            3583..>4934
                     /gene="mpl"
                     /locus_tag="VGR03_17290"
     CDS             3583..>4934
                     /gene="mpl"
                     /locus_tag="VGR03_17290"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR01081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEV1996091.1"
                     /translation="MSETKHVHVIGIGGSAMAPLAGMLRERGFRVTGSDSGVYPPAST
                     LLESLGISFFNTFDAAHLQPAADLVVIGNIIARGNPELEEVLDRKIPYRSMPEILEEV
                     FLPERHSIVVSGTHGKTTTTAMLAWIFHTAGKRPNFLVGGVAENFGKSYGLGGGEEFI
                     LEGDEYETAFWDRGPKFFHYHPDDLIITSLEYDHADIYPDFETYELAFRRLVNLVPRH
                     GRVVIWGDAEDSGPALRRAAEKAFCPVETYGFTSGNDWVASDFTIDGETMRFRVAHRG
                     KLFGEFALAATGRHNVLNAMGALVVAQGRGIAPKEIAKALATFRSVKRRMDVQGEIGG
                     VLVVDDFAHHPTAVKATIEAARGRWPGRRLWAILEPRSNSMRRKVFQEALPKALALGD
                     RVILGGVFRAQQLGGENRLDPETVAESVRALGKDARVFPSSDAIAEHLGTESKPGDIL
                     L"
BASE COUNT         1008 a         1342 c         1560 g         1024 t
ORIGIN      
        1 atcctgcttg gcggaactgg ccggagaggt tgcggaccga aagaaagatc cattttcagc
       61 cgtgaacgaa attctgaaga agagcggcct tcagggctag aggagaacaa aatatggcgg
      121 caaagaaggc caacaaatcg aagagagcaa aatccaatgc cacggcggcg atcggcatca
      181 tcggaggaag cgggctctat tccatgggcg gattgacgaa cacgcgcgaa attcgcgtga
      241 gaacgccatt tggcgatcct tcggatgcca tcgtgcttgg cacgctggaa ggaaagcgcg
      301 tggcgtttct ggctcgccac ggccgcgggc accgcatcct gccaagcgag atcaattatc
      361 gcgcgaacgt atacgcgatg aagctcctgg gcgtcgagcg catcatctcc gtgagcgcgg
      421 tgggttcgct gaaggaagat ctgcggccgg gagaatttct cgtcccagac cagtttttcg
      481 accgcacgaa gaaccgcgtc tccacttttt ttggagaagg aatcgttgct cacgtagcct
      541 ttgcgcaccc gacctgcggt caggtgtcgg gggttctggc ggatgcctgc gttcatgaag
      601 ccatcaaggt gcatcgccgc ggaacgtaca tctgcattga agggccgcaa ttttcgacgc
      661 tggcggaggc ggaagtgcat cggcagcttg gtttcgaagt gatcggcatg accaacgtga
      721 cggaagcgcg gttggcgcgt gaagcagaga tttgctacgc gacgatcgcc atgatcactg
      781 actatgactg ctggcatccg gagcacgaat cggtcacggc gtcgcaaatc atcgccacgt
      841 tgagtcagaa cgcggagaat gcgcagaagg cgctgcgaac cgccgttcga gagctgccca
      901 cgagcaggtc ctgcaaatgc gagtcggctc tcgagcatgc gctggtcacg gatttgaaga
      961 ttgtccccaa ggcaacaaag aagcggctcg cagcaatcat cggaaaatat atttcttagg
     1021 agtgcatcgt gtccttactc gtcgtgggtt ccgtcgcatt cgacgctttg gaaagtccgt
     1081 atggaaaggt ggatcgcgcg ctcggcggtg cggccacgta tttcgccgtg gcagccagtt
     1141 ttttcacgca cgtaaacctc gttgggatcg tgggagacga cttcacggaa gtggatgccc
     1201 agatttttca cggacgtact atcgatattg agggtttaga acgcgccacc ggcaaaacgt
     1261 ttttctgggc cgggcggtat tcgcaaaatt tgaacgagcg cgtaacgctg gcaacggagc
     1321 tgaatgtgtt ttcggaattc aagccccgct tgccggaaaa ataccgcagc gcaaagtacg
     1381 ttttcctcgc caacatcgcg ccggacttgc agcgggacgt gcttcaccag gtcaagaagc
     1441 gccccaaaat cgccgcgttg gataccatga actactggat cgagcgttcg aacgcggaac
     1501 tgcgtgaaac gctgaagcac gtcgacatcc tgatgatcaa tgattcggag acgcgcgagc
     1561 tttccagcga acacaatttg ctgcgcgcgg ccaagcacat tttcaagatg gggccggcga
     1621 cgctggtggt gaaacgcgga gagtacggcg ccatgatggt ggacaagcgt ggcgtcttct
     1681 gcgtgccggc gtttccgctc gaagagccgc acgatcccac cggcgccggc gacagcttcg
     1741 ccggcggctt catgggttac ctggcgggat gcggcgataa gagtgacgct tcgttgcgac
     1801 gcgccatggt ctacggttcc gtactgggga gctttgccgt cgaacaattt ggtctcgatc
     1861 ggctgcgtca cttgaagcgc agtgagatac acgccagggc acgccacttt tcgaaattga
     1921 cgcaatttaa actgtaaata gtaaaatttc gctcaggcgg gccgtgggtc ccgctggcga
     1981 tgctttctct tcaggaaata ttcttgcggc tgaccgtggc cgcggcgttg ggcgcggtca
     2041 ttggcattga acgcgatctg cggcggcggc ccgcgggcgt ccgcaccagc ctatttgttt
     2101 gcctggcgac ggcgttgttc accattcttt ccggcgaaat cgctcgccgc cttggcgata
     2161 cgggaagcac tcgaatcgct tcgaacattg tccagggcat cggttttctg ggcgcgggtg
     2221 cgattctacg cgaggcggga ggcgttatag ggatgaccac cgccgcgacc gtttttgtag
     2281 aagccgcaat cggcatggcc gctggcggag gtctctattc cgtggctgtc tatgccaccg
     2341 gcctcgtgct cttcggattg atctgcatcg gctgggctgc gcggcgcttt aatttgaagt
     2401 accgaatgat ggtgttccgc atcaccaccg gccacgcgga cagcgtcgcc acggaagtgc
     2461 agcggctgat ggccggcatg aaaattccgc tgcaacactt tcgcatttcc atggcgggcg
     2521 cgaactccat tgtcgaattc gaatccgacg tcagccacag ccagcaagaa acgattctca
     2581 cccagctcaa tcgccagggc gtgatcaccg aagtgattcc gctcgagggc catcacgaat
     2641 gacgggaagc aacgggactc cccgaaagcc caaagcgctt gctgccggtt cgcgcctggg
     2701 agtgttcgct ccggcgtctc cggcggagtc cgtcgacatg atcgccgggc ttgcggaatt
     2761 gaaacgccac ggttttcagg tggtcgccaa tcaggacacc aaagcggaag ggtactttgc
     2821 cggtccgccc ctggagcgga ccaatggttt tctcgggact ctgaattccg atcaagtcga
     2881 cgggctcgtc gctttgcgcg gcggctacgg atcgaattac cttctggaat tcgaactgga
     2941 gaagagcctc gccaatctaa aatgcgtgat tggcttcagc gacctcacca ctctgcagat
     3001 ttttctttgg cagcggtgta actgggtcac tttctacggc cccatggttg ccgccgggct
     3061 caacgcgggt ccgggtgcgc gtaagggcta tgacgagagc tcctttcttc aagcagtggg
     3121 cagaacggaa ggtggctgga agctgcggct gaggggtgaa gccgtcctcg cgggtcagtc
     3181 ccttggcaga gttctgggcg gatgcatgac tctgctggag gccaccatcg gaacgccatg
     3241 ggaattggaa accaaggatt caatcctggt tctcgaagac cgcgcaatga agccctacca
     3301 ggtggatcgc gtcctgatgc acttgaaaca ggccggaaaa tttgaaggtg tgcgtgggat
     3361 tgtcctggga gactttccgg aaagtgagcc ggctgcggcc ggcggcccaa ccgtccggga
     3421 agtctgcgcg cgaattctgc ggccactggg cgttccgatc gtgttcggag cgccggtcgg
     3481 gcataccgtg cggccgatgc tgacgatacc gcttggcatc aaggcgcgcc tggatgcgga
     3541 tggggaaggc acacttgaat ttctggaatc ggcggtggtg gcttgagcga aacgaaacac
     3601 gtgcatgtga tcgggatcgg cggatcggcg atggcgccgc tggccggtat gctgcgcgag
     3661 cgcggctttc gcgtgaccgg ttcggattcc ggagtctacc cgcctgcgtc gacgctgctc
     3721 gaaagtctcg gcatttcatt cttcaacaca tttgatgcgg cgcacttaca accggcagct
     3781 gacctggtgg tcatcggaaa tatcatcgcg cggggaaatc cggagctgga ggaagtgctg
     3841 gatcgaaaga ttccgtatcg ctccatgccg gaaattctgg aagaagtgtt tctacccgaa
     3901 agacattcca tcgtcgtcag cggaacacat ggaaaaacga ccacgacggc gatgctggct
     3961 tggattttcc ataccgcggg gaaacgcccg aattttctcg tggggggcgt agcggaaaac
     4021 tttggaaaga gttatgggct tggcggcggg gaagagttta ttcttgaagg tgacgagtat
     4081 gaaaccgctt tctgggaccg cggacccaag ttttttcact accatcccga tgatctgatc
     4141 attacgtcgc tcgaatacga ccacgccgac atataccctg attttgagac gtatgagttg
     4201 gctttccgcc gcttggttaa tcttgtgccc aggcacggcc gcgtggtcat ttggggcgac
     4261 gcggaagact cgggcccggc gctgcggcgc gcggcggaaa aggctttttg cccggtggaa
     4321 acctacggtt tcaccagcgg gaatgattgg gtggcgagcg acttcaccat cgatggcgaa
     4381 acgatgcggt ttcgggtcgc gcatcgcggg aaactgtttg gagaatttgc gctggccgcc
     4441 acgggacggc acaacgtctt gaacgcgatg ggggcacttg tcgttgccca ggggcgtggc
     4501 atcgcaccga aagagattgc caaagcgctt gcgacgtttc gcagcgtgaa gcgtcgaatg
     4561 gatgtgcagg gcgaaatcgg cggagttttg gtggtcgacg atttcgcgca tcatcccacg
     4621 gctgtgaaag cgaccatcga agccgcgcgc ggccgctggc ccggacgccg gctgtgggcc
     4681 attctcgagc cacggtcaaa ttccatgcgc cgcaaagtgt ttcaggaggc gctgccgaag
     4741 gcgctggctc tcggcgaccg cgtcattctc ggcggcgttt ttcgcgccca gcaattgggc
     4801 ggcgagaatc gcctcgaccc cgagaccgtt gcggaaagtg tccgtgccct aggcaaagac
     4861 gcccgcgtgt ttcccagttc cgacgccatt gcggagcatc ttgggaccga atccaaaccg
     4921 ggagatatcc ttct
//
LOCUS       DASYVO010000500         4159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_252488, whole genome shotgun sequence.
ACCESSION   DASYVO010000500 DASYVO010000000
VERSION     DASYVO010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252488"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VGR03_17295"
     CDS             <1..292
                     /locus_tag="VGR03_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996092.1"
                     /translation="KPARLFLTHFGFSDNPSEHILLFRERLHRWAALTAEILRTAASD
                     SAAMDSFMSATYAEISQHVPAGEAEHYAFSAGLNLSFLGLVRYLRKRATAAS"
     gene            complement(338..1324)
                     /locus_tag="VGR03_17300"
     CDS             complement(338..1324)
                     /locus_tag="VGR03_17300"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEV1996093.1"
                     /translation="MAVKIVRQPKKIALIGAPSSAAAFSAGSEKAPAALRAAGLAEKL
                     QAVGYEVVDYGDCAPRLFADDDEHRRARNLTEIVAGLNDLKTRAELAVKSGALVLVLG
                     GDCAQVIGLLTGARRYYKHVNLLWFDRDADLNTPASTPSGRIDGMVVAHIIGKGAPEL
                     VRFWSEAPLVREPDVTLFGLERLDPPEQEFLGKSPMRHIYAADIQSKGGSKAAHEALA
                     QVHADAREFVLHLDIDVVAEEDFPAVNVPGSDGLRFDDVRTAFVEFAKHKNLLGLDVA
                     QYNPDKDPDGSGAKKLVDLLVEALSARLEALAAPAPEPAAGSEEVSSSGTTA"
     gene            complement(1455..2420)
                     /locus_tag="VGR03_17305"
     CDS             complement(1455..2420)
                     /locus_tag="VGR03_17305"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEV1996094.1"
                     /translation="MPTATNGKVLLRVRPRGFCAGVVRAVDIVELALEAYGAPVYVHH
                     EIVHNTYVVEQLRRRGAIFVEAIEEVPHGAVLVFSAHGVPPTVRDEAKSRALKVIDAT
                     CPLVTKVHLEALKFAREKRTIILIGHRDHQEIVGTSGEAPDRTIVVDSVAAVDALEVN
                     DPDKLSFLTQTTLSLYDTQEIVARLRQKFPFIQGPASDDICYATQNRQEAVEQVAKDV
                     DLILVVGSPNSSNSNRLVEVAQRSGVKAQLIDSAKDIDVKWLEGVSRVGLTAGASAPE
                     VLVEQVSERLAEFGFTDQRDLDLIREDVRFTLPPELATIAPAPRR"
     gene            2629..3774
                     /locus_tag="VGR03_17310"
     CDS             2629..3774
                     /locus_tag="VGR03_17310"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEV1996095.1"
                     /translation="MSRTKSFWKTLPRTSFLVFLLGVFFIFITVAFASDVAEMGRQPA
                     LRFALHILLTGIFPVSYAVAGFALRKQFWKAFLPLFAVHFSLINILFRLFPAQPQPAQ
                     MDAAEIARLHDRLSFDGFAIMLAVALGYACFLYATITAGRRYLRVQAEIELATEIHRV
                     LVPAIDAKIEGYEFYGRSSPSGEVGGDLIDLAGSEDHWVAYVADVAGHGVAPGVVMGM
                     VKSAARMLLSSGDDSRHLLPRLNEVLYPLKKPDMFVTFCFLARNADGLRVGLAGHPAI
                     LHFSARTNAVTQLECPNMPLGIFPSGDFASSEVRTEPGDVFALYTDGFLEPANAAGEE
                     FGLMRLQGELQKHGKEPLDVICRSLQESVARHGAQFDDQSLFLIRKI"
     gene            3910..>4159
                     /locus_tag="VGR03_17315"
     CDS             3910..>4159
                     /locus_tag="VGR03_17315"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1996096.1"
                     /translation="MAAEMIVAPVTLEGTHVRLEPLAKTHLAGLAEVGLDEELWRWIP
                     TQVRTAEQMEAYIETALNEQERGTSLPFAIVEKDTGRAI"
BASE COUNT          790 a         1278 c         1226 g          865 t
ORIGIN      
        1 caaacccgcg cggctcttcc tcacgcactt cggtttctcc gataatcctt ccgaacacat
       61 cctgctgttc cgcgaacggc tgcatcgctg ggccgcgctc accgcggaaa tcctgcgcac
      121 cgccgccagc gattccgccg cgatggattc cttcatgtcc gccacgtacg ccgaaatctc
      181 ccagcacgtc cccgcgggtg aagccgaaca ctacgccttc agcgccggcc tgaatctctc
      241 ttttctcggc ctcgtgcgtt atttgcgcaa acgtgccacc gccgcttcct gaaccgcacg
      301 ccagttttct cttggttaga aacgaaatgc aggcggtcta cgcggtcgtt cctgacgagg
      361 acacttcttc cgaacccgcc gcgggctctg gggcgggcgc cgctagcgct tcgaggcgcg
      421 cggaaagcgc ttcgaccagc agatcgacga gcttctttgc gccgctgccg tcaggatctt
      481 tgtcgggatt gtactgcgct acgtcgagac ccagcagatt cttgtgtttc gcgaattcca
      541 caaacgcggt gcgcacgtcg tcgaaacgca agccgtcgct gccgggcacg ttcaccgcag
      601 gaaagtcttc ctccgcgacg acatcgatat caagatgaag aacgaattcg cgggcgtcgg
      661 cgtgcacttg cgccagcgct tcgtgcgccg ctttgctgcc acctttggat tgaatgtccg
      721 cggcgtaaat atgccgcatc ggcgatttgc cgagaaactc ctgctccggc ggatcgagcc
      781 tctcaagtcc gaagagggta acgtccggct cgcgcacgag cggcgcctcg ctccagaaac
      841 gcaccagttc cggcgcgccc ttgccgatga tgtgggccac aaccattccg tcgatgcggc
      901 cggagggagt gcttgcggga gtattcagat cggcgtcgcg atcgaaccaa agaagattca
      961 cgtgcttgta atagcgccgc gcgccggtga gcaagccgat gacctgcgcg cagtcgccgc
     1021 ccaagaccag caccagcgcg ccgcttttca ccgcgagttc ggcgcgcgtc ttcagatcgt
     1081 tcaggccggc gacgatttcg gtcagattgc gggcgcgccg gtgttcatcg tcgtccgcga
     1141 agaggcgcgg agcgcagtcg ccgtaatcca cgacttcata gccgacggcc tgcaactttt
     1201 cggcgagccc ggccgcgcgc agcgccgccg gagccttctc gctgcccgcg gaaaatgccg
     1261 cggcgctgga gggcgcgcca atcaaggcaa tcttcttcgg ctgacggacg attttgaccg
     1321 ccaaggtctg gctccttgtc ttgtatttcc cgcctaccgc gctagggtac cgatgaccgg
     1381 atcatttata gctcaggccg gcaaatcact ccagtcatgc actgcgcgtt cccctgccgg
     1441 gcactgcaaa ctctttagcg gcgtggcgcc ggcgcgatgg tcgccagttc cggcggcagc
     1501 gtgaagcgca catcctcacg aatcaaatcc aaatcgcgct gatccgtgaa gccgaactcc
     1561 gccagccgtt cgctcacttg ctccactagg acttccggag cggacgcgcc cgcggtcaag
     1621 cccacgcggg aaacgccttc gagccacttc acatcgatgt ccttcgccga gtcaatcaat
     1681 tgggctttga cgccgctgcg ctgggcgact tccacgaggc ggttggaatt cgagctgttc
     1741 ggcgaaccca caaccaggat caaatcgacg tccttggcga cttgctcgac cgcttcctgg
     1801 cggttctgcg tggcgtagca aatatcgtcg ctggcaggac cctggatgaa agggaatttc
     1861 tggcggaggc gcgccacgat ttcctgcgtg tcgtagaggc tcagggtcgt ctgcgtgaga
     1921 aacgagagct tgtcggggtc attgacttcg agggcatcca cggcggcgac ggaatccacg
     1981 acgatggtgc ggtccggcgc ttctccggac gtgccgacga tttcctgatg atctcgatga
     2041 ccgatcagaa taatggtgcg cttttcgcgc gcaaacttca gcgcctcgag gtgaactttg
     2101 gtcaccagcg ggcaagtggc gtcaatcact ttcagcgcgc gggacttggc ttcatcgcgg
     2161 accgtcggcg gaacgccgtg ggcgctgaaa accaacactg cgccgtgcgg cacttcctcg
     2221 atggcttcca caaaaatcgc gccgcgccgg cgcaactgct ctacaacgta ggtgttatgg
     2281 acgatctcgt gatgcacgta gaccggcgca ccgtaggctt ccaacgcgag ctcgacgata
     2341 tccaccgcgc gcactacgcc cgcgcagaaa ccccgcggcc ttaccctcaa caatactttt
     2401 ccattcgttg cagtcggcat tgtggtccta aatatatgat ttccgaagac tctatcatac
     2461 cttacggcgc gccccgcccg catcccgttt cctaatgacg gtagcggatc agtttacgaa
     2521 ttgggcgttt tacgccaggc ggcaaatggg ggcctgccca gcgcgagaga gaacgctgcg
     2581 cagctccccg atcggtatgg taggattcgc gaacgccccg cgaattctat gagccgaacc
     2641 aaatctttct ggaagactct cccccgaacg tcatttcttg ttttcctgct gggcgtcttc
     2701 ttcatcttta ttactgttgc ctttgctagt gacgtcgcag agatgggacg ccagccggcc
     2761 ctacgctttg cgttgcacat cctgctcacc ggcatatttc ctgtctccta tgccgttgca
     2821 ggatttgctc ttcgcaaaca gttctggaag gccttcctgc cgctctttgc cgtacacttt
     2881 agcctgataa acatactctt ccgattgttc cccgcccagc cacaaccggc gcaaatggac
     2941 gcggccgaga tcgctcggtt gcacgaccgg ttgtcctttg acgggttcgc catcatgttg
     3001 gccgttgcgc tgggctatgc gtgcttcctt tacgccacga ttaccgcggg caggcgctat
     3061 ctccgtgtcc aagcggagat cgaactggcc accgagatcc atcgcgtgct ggtcccggcg
     3121 attgatgcga aaatcgaagg ttacgagttc tacgggcggt cgtcaccgag tggcgaggtc
     3181 ggtggagacc tgatcgatct tgccgggtcg gaagaccact gggtcgccta tgtggcggat
     3241 gtggcgggcc acggggtagc acccggagtg gtcatgggca tggtcaagag cgcggctcgc
     3301 atgctgctga gctccggcga tgacagtagg catcttctcc cgcggctgaa cgaagtcctt
     3361 tacccactaa agaagccgga catgttcgtc accttttgtt ttcttgccag gaatgcagac
     3421 ggattgcgcg tcgggctggc aggccacccg gctattctgc atttttccgc cagaaccaac
     3481 gcagtaacac aattggaatg tccaaacatg ccattgggaa tttttccctc cggggatttt
     3541 gcaagctccg aggttcggac agagccggga gatgtcttcg cactctacac cgacgggttc
     3601 ctcgagccag cgaatgccgc cggggaagaa tttgggctca tgcggctcca aggagaattg
     3661 cagaaacatg gcaaggagcc cttggacgta atttgcaggt cactgcagga gagcgtcgcg
     3721 cggcacggag cgcaatttga cgaccagtcg ctcttcttga ttcgaaagat ataggatgcg
     3781 ttccacggag aggccgcgaa aaacgtgact gcgccttgcg agacctcaca ctcagactgt
     3841 gcgcccgtta ggacatcgcc aaattggttc tcgctgaagg agcaaggttg cccttagaat
     3901 gcagccacta tggcggcaga aatgattgtt gcgccggtga cgctggaagg cacgcatgtg
     3961 cggctggagc cgctggcgaa gacgcatttg gcgggactcg ctgaggttgg cctggatgaa
     4021 gaattgtggc ggtggattcc cacgcaggtg cgaacagcgg agcaaatgga ggcgtacatc
     4081 gagaccgcgc tcaacgagca ggagcggggc acatcgttgc cgtttgcgat cgtggagaag
     4141 gacacaggtc gcgcgatcg
//
LOCUS       DASYVO010000501         9274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_140527, whole genome shotgun sequence.
ACCESSION   DASYVO010000501 DASYVO010000000
VERSION     DASYVO010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9274
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140527"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VGR03_17320"
     CDS             <1..449
                     /locus_tag="VGR03_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015212237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1996097.1"
                     /translation="LSGGQKQRVSIARAILADPRILILDEATSSLDSESEALIQEGLR
                     YLMRGRTTFVIAHRLSTIRRADQILVVEAGRVIERGIHETLYALGGRYFDLYTKQHAV
                     EANLFLAPGEGSDLEEARGVEIQAGGNGRSDAALPDAIRIIRGQSS"
     gene            complement(502..1296)
                     /locus_tag="VGR03_17325"
     CDS             complement(502..1296)
                     /locus_tag="VGR03_17325"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV1996098.1"
                     /translation="MNFFIGFLIAVAVGLTGIGGGSFTVPALVLLVGLTAGEAVGTAF
                     VFAGVLRLIAAPFYLVGKNFHARYLRLLLLGAVPGLLLGTFVLRLLGEQGGNPAVIIL
                     IGLLLLASSSVTFAPRVQNPGFARKNSRWLPWLALPIGVEYGFSSAGAGALGSVLLLN
                     YSEITTPQVIGTDLIFGLVLAAIGGAFHWKFGAINSPVLLQLLAGGVPGVVFGCALAR
                     YVPARKLKLVVAMIAIFAGVQLVWNGTRTLLANRAAAAVKMAVRHQ"
     gene            complement(1293..1994)
                     /locus_tag="VGR03_17330"
     CDS             complement(1293..1994)
                     /locus_tag="VGR03_17330"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEV1996099.1"
                     /translation="MDVDRISEVVESLAPEQALGVIFAAKENARICLTSSFQAEDMTV
                     LHLLRKRIPDVPVLFLDTGYHFPQTYEYRDRMAKEWSLHLVNVLPARTVAEQESALGV
                     LNQTDPTRCCQLRKVEPLLGALEPFDVWFTGLRREQSPTRKNLKKVELHRLPTGKTLW
                     KVSLLADWNWEQVWHYVNAIGISHLPQYDEGYLSIGCQPCTALPADPNNPRSGRWGGT
                     KLECGIHTFSKRAEG"
     gene            complement(1995..3752)
                     /gene="cysN"
                     /locus_tag="VGR03_17335"
     CDS             complement(1995..3752)
                     /gene="cysN"
                     /locus_tag="VGR03_17335"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEV1996100.1"
                     /translation="MISSVKSGPRDDFAGFFWQAPNTELLRFTTAGSVDDGKSTLIGR
                     LLHDSKAVYEDQLASVKKSRINRSTGPVDFSLLTDGLRAEREQGITIDVAYRYFATAR
                     RKFIIADTPGHEQYTRNMATGASTADLAVILIDGTKGLLPQTRRHAFIASLLGITNVL
                     AAVNKMDLAGYSEDVFLELQSDFLALAAQLGIANVQCVPISALEGDNVVDRSKRMPWY
                     QGPTLLAHLETVPLPEKDSLQGFRFPVQTVIRPDATFRGFAGRIASGVIHPGDPVVAL
                     PSRQKSFVRSIVTYDGELPQASSSMSVTLQLDEEIDLSRGDMLVSPDHLPRVSRKFHA
                     MVVWLNGAALELGRTYFVKHTARQTKIRALHIRHRVNVNTLAEEPAARLEMNEIGSVE
                     FETHVPLFFDPYSSNRMTGSFILIDAATNATVGAGMIQEDVSAVGSAGEPEKEFASSA
                     VDETPVAAEERYARHGHFPAVVLLEGRAALAQRLERLLFELGFEVLDLSRTEFTGDTF
                     AVAIRVARTAGLIVLYSGDALDGETKQRLAVEFDNRFFDLAGALDLHVDEERATAHVL
                     ALAQSRWLVEPRKHQERLN"
     gene            complement(3752..4729)
                     /gene="cysD"
                     /locus_tag="VGR03_17340"
     CDS             complement(3752..4729)
                     /gene="cysD"
                     /locus_tag="VGR03_17340"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEV1996101.1"
                     /translation="MTNASEVLIRQPEPASCGAVEARTAAAPRLNHLRALESESIHIL
                     REAAAEFARPVMLYSIGKDSSVMLRLAQKAFYPGKIPFPLLHVDTSYKFPEMIEFRDS
                     YPRQIGAELIVHKNQEALDSGANPYSLGTQKCCGLLKTKSLLDALAEGGFDAAFGGAR
                     RDEEKSRAKERIYSFRDSLGQWDPKNQRPELWNIFNSRLDKGESIRVFPLSNWTELDV
                     WLYIEAEKIPIVPLYFAKERDVVLRNGSIVVIDHTAQLLPGEKTQRLKCRMRSLGCVP
                     CTGAIRSDADTVPQIIEEMVSFRRSERENRAIDHDEEGSMELKKREGYF"
     gene            complement(5134..5553)
                     /locus_tag="VGR03_17345"
     CDS             complement(5134..5553)
                     /locus_tag="VGR03_17345"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1996102.1"
                     /translation="MPKEKPRVNIGEVIRTYRGQRSLSQGDIERRTGLLRCYLSRVEN
                     GHTVPSLETLAKIAEAMDISLADFFPGTETPRDRETQKALGELSQDEIRFLAEIKKYS
                     TTLSDGDKRLVLAMIRKMATLIPPPAAKKMIVRRPTA"
     gene            complement(5768..6580)
                     /locus_tag="VGR03_17350"
     CDS             complement(5768..6580)
                     /locus_tag="VGR03_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996103.1"
                     /translation="MNRFLSVFALGLFFVGAAAADTLELKDGRILKGKYLGGTQAVLR
                     FELNGEVQTFSTTEIVALTFTGNSGYSAPVAAPAPAKAQAPESAPRMAISGSSDSVTI
                     PAGQSLLVRMIDGVDSSKNHVGDVFHASLETDLDIGNFVVARKGTDIYGRLANAQEAG
                     HMSGSAELQLELTRIVIDGKDYPVVSSDYSLRGKGRGSDTAKKVGGGAVAGAIIGAIA
                     GGGAGAAIGAGVGSAAGAGVQVFTRGQQVKVPSETLLEFRLQQPVTVAPSQR"
     gene            7011..7382
                     /locus_tag="VGR03_17355"
     CDS             7011..7382
                     /locus_tag="VGR03_17355"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEV1996104.1"
                     /translation="MNDAQSATLVVRFGNFEVNLHSRELRKHGMRIRLEEKPFQILEM
                     LLDRAGHVVTRRTLRERLWPDTYVGYEHGLNTAVNKLRDLLGDSARNPRFVETLPRLG
                     YRFIAPVVQPRKVLAPELLPG"
     gene            complement(7379..>9274)
                     /locus_tag="VGR03_17360"
     CDS             complement(7379..>9274)
                     /locus_tag="VGR03_17360"
                     /inference="COORDINATES: protein motif:HMM:NF037222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEV1996105.1"
                     /translation="GKVRNVSNEPVKGVLRTEVDGMELQQAVELNGGESKTVIFGPEQ
                     FAKLKLAHPRLWWPYQMGTPNLYTAKLSFEIGKQISDAASVTFGIREVTSELTDKGHR
                     LFKVNGRRVLIRGAAWAPDLLFRWSSARLDADLAYVRDMGMNTIRLEGRLDRDEFFEK
                     TDRLGILVMPGWTCCDAWEKWDNWKGDQSEVAAASLRDQITRLRNHPSVFVWLNGSDN
                     PPPAIVERMYLDIEKELQWPNPVVSSASEQKAAVTGESGVKMTGPYEYVPPVYWLADT
                     EAGGAHGYNTETSPGPAIPTRESLEKFIPKEHLWPIDEVWNYHAGGERFTTVNVFTEG
                     LNRRYGPAASLDDYERKAQAMTYDGQRAMFEAYARNKYTSTGVIQWMLNNAWPSLIWH
                     LYDYYLVPAGGYFGTKKACEPVHVQYSYDDNSVAVINGTYEMLKGTKVSAKIYSIDAK
                     EKASREATFDLGADSSTKAFDLPQPDGLTPTYFVKLELHDAAGKLVSENFYWLSTKPD
                     VLDWAKRSDTVYTPQKEFGDLTGLNSLPKAKIAITKRVHASGGNSVLTVAAENKSDSV
                     AFMVHPRLTRGKGGEDVTPVFWSDNYFSLLPGEKKAVTARFDFASLHGATPVLVVEGW
                     NVEPTAP"
BASE COUNT         1936 a         2816 c         2641 g         1881 t
ORIGIN      
        1 agctctccgg cggccagaag cagcgcgtct cgatcgctcg cgccatcctg gccgatccgc
       61 gcatcctaat cctcgacgaa gccacttcca gcctcgattc ggaatcggag gcgcttatcc
      121 aggagggtct gcgctacctc atgcgcggcc gcacgacctt tgtgatcgcg caccggctat
      181 ccacgattcg gcgcgccgat cagattctcg ttgtcgaggc ggggcgcgtc atcgagcgcg
      241 gcatccacga aaccctctac gctctcggcg gccgctactt cgatctctac accaagcagc
      301 acgccgtcga agccaacctt ttcctggcgc cgggggaagg tagcgatctg gaagaagcca
      361 gaggcgtgga aatccaggca ggcggcaatg gccggagcga tgccgctcta cccgacgcca
      421 tccgcatcat tcgcggacaa tccagctaga attccattcg agattatttt tcggcgaaac
      481 cgcaggccgc ggctatttta cttattggtg ccgaaccgcc atcttgactg ccgccgccgc
      541 tcgatttgcc agcagcgtgc gagtgccgtt ccagacgagc tgaactccgg cgaaaatggc
      601 gatcattgcg acaaccaatt ttagctttcg cgcgggtacg tagcgggcca gagcacagcc
      661 gaatacgaca ccaggtactc caccggcaag gagctggagg agaaccggag agttgatcgc
      721 gccaaacttc caatgaaacg ctccgccgat cgcggccagc accagaccaa aaatcaggtc
      781 cgtgccaatg acctggggag tggtgatctc cgagtagttt aagagtagaa cgctcccgag
      841 cgcaccggct ccggcggagg agaatccgta ttccacaccg atggggagcg cgagccatgg
      901 taaccagcgg gaattcttcc gggcgaaacc tggattctgc acgcggggag caaacgtcac
      961 gcttgacgat gccaagagca agagtccaat caggatgatg acggccggat ttcctccctg
     1021 ttccccaagg agccggagaa caaacgtgcc gagcaggagg ccggggacgg ctccgagcaa
     1081 aagcagcctc agatagcgcg cgtgaaagtt ttttcccacg agatagaagg gggccgctat
     1141 caggcgcagg acgcccgcga acacgaacgc cgtgcccacc gcttcaccgg ccgtcaagcc
     1201 aacgagcaag acgagtgccg gaacggtgaa gctgccgccg ccaataccgg tcagacccac
     1261 ggcgacggca atcaggaatc cgataaagaa gttcatcctt ccgctcgctt cgaaaaggtg
     1321 tgaatgccgc attccagctt cgttccgccc cagcgcccgg atctcgggtt gttcggatca
     1381 gctggcagcg cggtgcaggg ctgacatcca atgcttagat aaccctcgtc gtactggggc
     1441 aggtggctga tgccaattgc attcacgtag tgccaaacct gttcccaatt ccagtccgcg
     1501 aggaggctca ccttccagag cgttttgcct gtagggagac ggtggagctc gaccttcttg
     1561 aggttcttgc gcgtggggga ctgttcgcgc cgcaatccgg tgaaccaaac gtcgaagggc
     1621 tctagcgcac cgagaagagg ctcgactttg cgcaattggc agcagcgagt gggatcggtc
     1681 tggttaagaa cgccaagagc tgattcctgc tccgccacgg ttcgcgccgg caaaacgtta
     1741 accagatgca gcgaccattc cttggccatc cggtcgcggt attcgtaggt ttgcgggaag
     1801 tgatagccgg tatccaggaa caacacgggc acatccggaa tccgcttgcg caggagatgc
     1861 aggacggtca tgtcctcggc ttgaaagcta ctggtcaggc aaatgcgggc gttttccttg
     1921 gccgcgaaaa tgacgcccaa agcctgctcc ggggcaagac tctcgacaac ttcggaaatc
     1981 cgatcaacgt ccattcaatt caacctttcc tgatgctttc ggggctcaac aagccaccga
     2041 gattgcgcaa gcgccaggac atgcgcagtg gctcgttcct cgtcgacgtg gagatccagc
     2101 gcgccagcca agtcaaaaaa acgattgtca aattccaccg cgaggcgctg ttttgtttcg
     2161 ccatcgagag cgtcgccgga atagaggacg atgagcccgg cggtccgcgc cacacggatc
     2221 gccacagcaa acgtatcgcc ggtgaattca gtgcgactga gatccaacac ctcgaatccg
     2281 agttcgaaaa gtagacgttc gagccgttgg gcgagcgccg cgcggccttc gagtagaacg
     2341 accgccggaa aatggccatg acgagcgtat cgttcctcgg ccgcaaccgg agtttcgtcc
     2401 acggcggagg aggcaaattc cttctcaggt tctcccgcgc tccccaccgc ggacacgtct
     2461 tcctgaatca tccccgcgcc caccgtggcg ttcgtggcag cgtcgatcaa aatgaagctg
     2521 cccgtcattc ggttactcga gtagggatcg aaaaacagag gcacatgggt ctcgaactcc
     2581 acggaaccga tttcattcat ctccaaacga gccgccggct cttccgctag cgtattgacg
     2641 ttgacgcgat ggcggatgtg gagagcccgg atcttcgtct ggcgagcggt gtgtttgacg
     2701 aaataggtgc ggcccaattc gagggcggcg ccgttaagcc agaccaccat ggcatggaat
     2761 ttccgggaga ctctgggcag gtggtcaggc gataccagca tgtcgcccct gcttaggtcg
     2821 atctcctcat ccaattgcaa ggtcacggac atggaggaag acgcctgcgg cagttcgccg
     2881 tcgtaggtca cgatggagcg cacgaaagac ttttgcctcg agggaagcgc aacgacagga
     2941 tcaccgggat gaataacgcc gctcgcaatg cggccggcga agccgcgaaa cgtggcgtcc
     3001 ggacgaataa cggtctgcac cgggaagcga aagccttgaa gcgaatcctt ttcgggaaga
     3061 ggaacagttt ccaggtgcgc cagcagcgta gggccttgat accacggcat ccgcttgctt
     3121 cggtcgacga cgttgtctcc ctccagcgcg ctgatcggca cgcattgcac gtttgcgatt
     3181 cccaattgcg ccgcgagcgc caggaaatcg ctctgcagtt ccagaaaaac atcttcgcta
     3241 tacccggcca gatccatctt gttcacggca gcgagtacgt tggtaattcc cagcagagaa
     3301 gcaatgaagg cgtgacgccg cgtttgcggg aggagcccct tggtgccatc aatcagaatg
     3361 accgccaggt ccgcggtgga tgcgcccgtc gccatgttcc gcgtgtactg ttcatgcccc
     3421 ggagtgtcgg cgatgatgaa tttgcggcga gccgtggcga aatagcggta agccacgtcg
     3481 atggtgatgc cctgctcgcg ttcggcccgc agaccatccg tgagcaacga aaaatcgacc
     3541 ggccccgtgg agcggttgat gcggctcttc ttcaccgagg cgagctgatc ttcgtagacg
     3601 gccttggaat cgtgcaagag gcgcccgatc agagtcgact tgccgtcgtc gacgctcccc
     3661 gccgtggtga agcgcagaag ctcggtgttc ggggcctgcc aaaaaaaacc ggcgaaatca
     3721 tcgcgggggc cgctcttcac ggaagaaatc atcagaagta tccctcccgt ttcttgagtt
     3781 ccatcgagcc ttcttcgtcg tggtcgatgg cgcgattctc gcgttcggag cggcggaacg
     3841 aaaccatttc ttcgataatc tgcggcacgg tgtcggcatc ggaacggatc gctcccgtgc
     3901 agggaacgca tccgagggac cgcatgcggc acttcaggcg ctgcgttttc tctcccggca
     3961 ggagctgcgc ggtgtgatca atcaccacaa tggaaccgtt gcgcaagacc acgtcgcgtt
     4021 ccttggcaaa atacagtggc acgatcggaa tcttttccgc ttcgatgtag agccaaacgt
     4081 cgagctcggt ccagttggaa agcggaaata cgcgaatgct ttcccctttg tcgaggcgag
     4141 agttaaaaat attccacaac tcgggccgct ggtttttcgg gtcccactgt cccaaggaat
     4201 cccggaacga atagatgcgc tccttggcgc gtgatttctc ctcgtcgcgg cgcgctccgc
     4261 cgaacgccgc gtcaaaaccg ccctcggcca gagcgtccag aagcgacttc gttttcagca
     4321 gcccgcagca tttctgcgta ccgagcgaat aggggttagc accggagtcg agcgcttcct
     4381 gattcttatg aacgataagc tccgcgccga tctgccgggg ataagagtcg cggaactcga
     4441 tcatctcggg gaatttgtaa ctggtgtcca cgtgcagcaa aggaaaagga atctttcccg
     4501 gatagaaagc cttttgcgcc aggcgcagca tgaccgaaga gtcttttccg atcgaataga
     4561 gcatcaccgg gcgcgcgaat tcggcggcgg cttcgcgcag gatgtgaatg ctctccgact
     4621 ccaacgctcg caggtgattg agcctaggtg ccgcggccgt tcgcgcttcg accgccccgc
     4681 aggatgcagg ttcgggctgc cggattaaga cttccgatgc gttggtcatg aatgtcctct
     4741 aaaacaaaaa acccacctgc cggaattcct ggcggtgggt tcgctacaat cttggagtag
     4801 agagctttta gaaagctggc ttcccatcca aacaagacgc ggcgaccgtc accgttgggt
     4861 gcggacaaca acaaacgccg gtcattcgga tggaagtcat tccgatggtt agaataccgg
     4921 gaagcccccg ggttgaccaa tcatcttttt ggtttgatga catcattatt ttggatgctt
     4981 gccgctcgcg ccctaacgta aagatgagaa tcgtcctcaa gcgcgtgaga tggttttcag
     5041 gtggcgccga atcgccgagg ttgaacggcc acccgatcgg tccgcgcctg cccgtaaatc
     5101 tcgatgaggc gaaccggagt tcgtgctgcc cggttacgca gtggggcggc gcacaatcat
     5161 cttcttcgcc gcaggtggtg gaatcaacgt agccatcttg cggatcatcg ccagcaccag
     5221 ccgcttgtcc ccatcggaga gcgtggtgct gtacttcttg atttccgcca ggaagcgaat
     5281 ttcgtcttgc gagagctcgc cgagcgcttt ttgcgtttca cggtcgcgcg gcgtttccgt
     5341 cccggggaaa aagtccgcca gcgaaatgtc catggcttcc gcgatcttgg caagcgtttc
     5401 gagagacggg acggtgtgcc cgttttcgac gcgcgacagg taacaacgca gcagcccggt
     5461 gcggcgctcg atgtcaccct gcgacaggct acgctggccc cgataggtcc ggattacttc
     5521 cccaatgttt actcttggtt tttctttcgg catggtctcc ccacttgagg gggtagctta
     5581 ctcactaaca gcaggagtgg caagagggaa tttctggcgg atggttccaa tgcgataatg
     5641 gctgaggaaa acgcggtgag gacatgcccc tctgaaaaaa gaggaaggaa aagaaaaagg
     5701 cgagccggag cccacattta ttcagaatat cctccgggac ttcaataagc accagcgaga
     5761 atcacactta cctttgcgac ggcgccaccg ttaccggttg ctgcaggcgg aattccagaa
     5821 gcgtctcgct cggcactttt acctgctgac cacgcgtgaa tacctggacg cccgcgcccg
     5881 cggcggaacc aacccccgca ccgatcgccg cgcccgcgcc gccgccggcg atcgcgccga
     5941 tgatcgcgcc ggccacagcg ccaccgccca ctttcttcgc ggtgtctgaa ccgcggccct
     6001 ttcctctgag gctgtagtcg ctgctgacga ccggatagtc cttcccgtcg atgacgatgc
     6061 gcgtcagttc gagctgcagc tcagcgctgc cggacatgtg ccccgcttct tgagcgttcg
     6121 cgagccgtcc atatatatcg gtgcccttcc gtgccaccac gaaattgccg atgtccaaat
     6181 cggtctccag gctggcgtgg aatacgtcac ccacgtggtt ttttgaggag tccactccgt
     6241 cgatcatgcg caccagcaga gattgtccgg ctgggatggt tacgctatcg ctggaaccag
     6301 aaatcgccat gcggggcgcc gactccggag cctgcgcctt cgccggagct ggagcagcta
     6361 caggagcgga atatccggaa ttcccggtga acgtcagcgc cacgatctcc gtggtactga
     6421 aagtctgaac ttccccattt agctcgaaac gcagcacggc ttgcgtgcca ccgagatact
     6481 tccctttcaa aatccggcca tcttttaatt ccaaagtatc cgccgcagcg gcgccgacga
     6541 aaaacagccc cagcgcaaac acagacaaaa aacgattcat tctcttcatt gttccctctc
     6601 gcgatcggaa atctcaactc cgggagatct ccgcactcgt ttcccgcacc caaaggcacc
     6661 ttcacaattc taccgtattg gatggcgggg acgcggcgaa tggttgcgcg aaattccacc
     6721 gtgagaaaag tcaggaggag gaagggatag agaagaaggt ataagcgaac cggcctggac
     6781 cctcggttct aactagccag tcagggccgc gtacacctgc cgtcactcct gcaaagggtt
     6841 ttgggcgctt agatggcaaa cggcggctgg gaataaatca gggaacggac aagtctccat
     6901 acacaaccag cctgtggcgt gtaagggctt atcagaaagg gtcacgggtg atcggatggg
     6961 accggcgggc aaccccgggt cgaggccggg caacccgcca ggatgaacga atgaacgacg
     7021 ctcagagcgc aaccctcgta gtgcgttttg gcaacttcga agtcaatctc cactcgcgcg
     7081 aacttcgcaa gcatggaatg cgcatccggc tggaagagaa acccttccaa attctggaaa
     7141 tgctgctgga ccgcgccggg catgttgtaa cccggcgaac gctccgcgaa agactctggc
     7201 ccgacactta tgtcggctac gagcacgggc tgaacaccgc cgtcaacaaa cttcgcgatc
     7261 tgctggggga ctccgcgcgg aatccacgtt ttgtcgagac cctccctcgg ctcggctatc
     7321 gcttcatcgc tccggtggtc cagccgcgaa aggtgctggc gcccgaattg ctccctggct
     7381 agggcgctgt tggctccacg ttccagccct ctaccaccag tacgggtgtc gccccgtgga
     7441 gtgaggcgaa atcaaatctc gccgtgacgg cttttttctc ccccggcagc agggagaaat
     7501 agttatcgct ccagaaaacc ggcgtcacgt cttccccgcc tttgccgcgt gtgagccgcg
     7561 gatggaccat gaacgccacg ctgtcgctct tgttctcggc ggccacggtg agcacggagt
     7621 tgcccccact cgcatgcact ctcttggtga tcgctatttt tgctttggga aggctgttca
     7681 gcccggtgag gtcgccgaat tccttctgcg gtgtgtagac ggtgtcgctt ctcttggccc
     7741 agtcgagcac gtctggtttt gtcgacaacc aatagaaatt ctcgctgacc agctttcctg
     7801 cggcgtcatg gagctcgagc ttcacgaaat acgtcggcgt cagtccatcc ggctggggga
     7861 gatcgaacgc cttggtgctg ctgtccgcac ccaaatcaaa cgttgcctcg cgcgaagctt
     7921 tttccttcgc atcgatgctg tagattttcg cgctgacttt cgtgcctttg agcatctcgt
     7981 acgttccgtt gatcaccgcg acggagttgt cgtcgtacga atactgcaca tggaccggct
     8041 cgcaagcttt tttcgtcccg aaatacccgc ccgccggcac caggtagtaa tcgtagagat
     8101 gccagatcaa ggaaggccac gcgttgttca gcatccactg aatcacgccc gtggacgtgt
     8161 atttgttgcg cgcgtaggct tcaaacatgg cgcgctggcc gtcgtaggtc atggcttgcg
     8221 ctttgcgctc gtaatcatcg agcgacgccg ctggtccgta gcgccggttc aatccttcgg
     8281 taaaaacatt gacggtcgtg aagcgctcgc cacccgcgtg ataattccag acctcatcga
     8341 tgggccagag atgctccttg ggaatgaatt tctccagtga ttcccgagta gggatcgccg
     8401 gccccggact cgtctccgtg ttatagccgt gagcgccgcc cgcttcggtg tcggccagcc
     8461 aatacaccgg cggcacatac tcgtaggggc cggtcatctt tacacccgat tctcccgtga
     8521 ccgccgcctt ctgttccgaa gcggacgaga ctaccgggtt cggccactgc aactctttct
     8581 cgatatccaa atacattctt tcgacgattg cgggcggcgg gttgtcgctg ccgttcagcc
     8641 acacaaaaac gctcggatga ttccgcagcc gggtgatttg gtcacgcagc gaagccgctg
     8701 caacttcgct ctgatcgccc ttccagttat cccacttctc ccaggcatcg cagcacgtcc
     8761 accccggcat gaccagaatc cccagccggt ccgtcttctc aaaaaattcg tctcggtcca
     8821 accgcccttc caggcggatg gtgttcatgc ccatatcgcg cacgtaggcc aaatccgcgt
     8881 ccagccgcgc cgacgaccac cggaaaagga gatccggcgc ccaagccgcc ccacggatca
     8941 atactcttct cccgttgact ttgaacaacc ggtggccctt gtccgtcaat tccgaggtaa
     9001 cctcgcgaat tccaaacgtc acgctcgccg cgtccgaaat ctgcttgccg atctcgaacg
     9061 acaacttcgc cgtataaaga ttgggcgtcc ccatctgata gggccaccac aaacgcggat
     9121 gcgccagttt caactttgcg aactgttccg gtccaaatat cacggtcttc gattcgccgc
     9181 cgttcagttc caccgcttgt tgcaactcca tcccatcgac ttccgttcgc agcactcctt
     9241 tcacgggttc attcgaaacg ttgcgcactt tccc
//
LOCUS       DASYVO010000502         5507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_279699, whole genome shotgun sequence.
ACCESSION   DASYVO010000502 DASYVO010000000
VERSION     DASYVO010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279699"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VGR03_17365"
     CDS             <1..180
                     /locus_tag="VGR03_17365"
                     /inference="COORDINATES: protein motif:HMM:NF013038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HEV1996106.1"
                     /translation="VTMIEVTDIEGAQVGDVATVHGTDGKQVLPANRVARSIGTVTSD
                     LLCAVSQRVPRLYVR"
     gene            378..1652
                     /locus_tag="VGR03_17370"
     CDS             378..1652
                     /locus_tag="VGR03_17370"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEV1996107.1"
                     /translation="MSRISPEAQRRSHSPETRVSSQREDRAIHPRPGWASRVGWDFWV
                     VRAFLFACTGAVSYTLGPFGLRGFASAGVGFLIALVVLLAELRLRRAALSGLLGGAFG
                     AVLGVFAALLVTLVISRTDEPEPTKSFLEFAALFAFGYLGLVLGSRRGGEMHVEALDG
                     FFGKKSTPAESLKLLDTSVLIDGRIADICDAQFLEGVLGVPQFVLHELQMVADSSDPL
                     KRQRGRRGLEVLQRIQKMPQVEVRILEEDFPQAGDVDHKLVELARRTGAKIVTNDFNL
                     NKVATVQGFSVLNVNLLAHALKPVVLPGEPMRVLILREGKEPNQGVAYLDDGTMVVVD
                     GARRMINKSVDIIATSVHQTTAGKMIFGRLEERAEQAAPAMRQAAAAGRGDGSGSVRS
                     DATSRVETSARLPEQPTLDRQARPKFPEPDAS"
     gene            1760..2476
                     /gene="ispD"
                     /locus_tag="VGR03_17375"
     CDS             1760..2476
                     /gene="ispD"
                     /locus_tag="VGR03_17375"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV1996108.1"
                     /translation="MSRIAAILPAAGMGTRMGAETPKQFLELDGTPIVILSLRRIAAC
                     PQVTDIIVATRADEVGRLEERIKQEKFKQAVRVVKGGDSRQDSVAAALREVANDTEIV
                     LVHDAVRPFVTPEQIARVIEEARRCEAAILGIPAMDTVKEVKRASLPEDVALITGTVP
                     RERVVLAQTPQAFSTKLLKEAFARAEADGVNVSDEAGLVERIGHDVHVVLGSERNMKI
                     TKPADMELARFYLERERQKA"
     gene            2473..2952
                     /gene="ispF"
                     /locus_tag="VGR03_17380"
     CDS             2473..2952
                     /gene="ispF"
                     /locus_tag="VGR03_17380"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEV1996109.1"
                     /translation="MSTRCGIGYDLHRLEEGRKLIVGGIELPFDKGPVGHSDGDVLAH
                     ALCDALLGAAGLGDIGTHFPDTDPKWKGANSMLFLAHAKKLLDEKHFAIEHVDAVIIT
                     EKPKLGPHFPKMREALAKALSVGVEKIHLKAKTNEGVDAIGRGEAIAAHVIATLTSR"
     gene            2968..3585
                     /locus_tag="VGR03_17385"
     CDS             2968..3585
                     /locus_tag="VGR03_17385"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996110.1"
                     /translation="MQGRRIPSLALRIKLRRYATILLMLPADHSDAPVAAANPFTAAD
                     VLGILRERGWLAAEPSLEQQAWCERAAAMLGGHATDRAALGDLLGLVFQYDAREIISR
                     VESHVVLSRYAARDVLRQVALLLLDGAPLTSERFKEIVTALKDGMALRGRELFHPIRL
                     ALAGRAGEGELDRVILLLDEAAAVSFTMPVKSARARILEFCSALD"
     gene            3762..4511
                     /gene="rlmB"
                     /locus_tag="VGR03_17390"
     CDS             3762..4511
                     /gene="rlmB"
                     /locus_tag="VGR03_17390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV1996111.1"
                     /translation="MDKLTGIHAVKEALEARRPIDRIAIAKGRQDTRVEEIVQLARQQ
                     GVPVRFEDRGQLDRLANSKDHQGVVALAAARAAATLEDILALANAGPGQLGLIVLLDG
                     VEDPHNLGAIIRTALAAGAHGVVIPERRAAGLTDTVARASAGALAHLPVAKVTNLVRT
                     MEELKEAGYWLVGLDEQGDKNYTEVDYTSPVGIVLGSEGQGLHELTRKRCDFVVSLPT
                     TGPVKSLNVSVAAGVVLFEAIRQRNMRAREK"
     gene            complement(4553..5074)
                     /locus_tag="VGR03_17395"
     CDS             complement(4553..5074)
                     /locus_tag="VGR03_17395"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV1996112.1"
                     /translation="MNDLKKLIREVPDYPKPGILFYDLTTLLKDKQGFHMLIDRLCEH
                     YQGHTIDVVVGIEARGFIFAPALAYRLGAGFVPVRKPKKLPAKTASISYALEYGTDTL
                     EIHEDAVKPGQRVIICDDLLATGGTAAATAKLIQQLGGTVEGAAFAVELTFLNGRSKL
                     NGLDVFSLIQYDK"
     gene            complement(5118..5411)
                     /locus_tag="VGR03_17400"
     CDS             complement(5118..5411)
                     /locus_tag="VGR03_17400"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV1996113.1"
                     /translation="MSDAKLAKRYIVSGMVQGVGFRYFTQAAAEKLRVSGFVRNLGDG
                     RVEVFAMGAPQQHAEFRAMLERGPRFSSISEVHEETAVPDPQYEDGFVINPSA"
BASE COUNT         1038 a         1526 c         1827 g         1116 t
ORIGIN      
        1 gtaaccatga ttgaggtcac ggacatcgag ggcgcgcagg tcggcgatgt agccacggtc
       61 catgggacgg acggcaagca agtgctgccg gcgaatcgcg tggcgcgcag catcgggaca
      121 gtcacctcgg atctgctgtg cgcggtgagc cagcgcgtgc cgcggctcta cgtccgataa
      181 ttggaaaatt ctatccgctg cattcggcat ttccgctgct gccggcttga tgcccgcttg
      241 acgccacact gttcagagag tatataaatc tcggtaacgc gaaccggcgt tccacatccc
      301 gcatcggccc actcctctcc gctcctgcgg gctggctgta ctgattttgc agtgaaagct
      361 cgatactgtg aggtcgtatg agccggatca gtcctgaagc acaacggcgt tcgcattccc
      421 cggaaaccag agtttcgtcc cagcgcgaag atcgtgcgat ccacccgcgt cctggctggg
      481 catcgcgtgt gggttgggac ttctgggtgg tgcgcgcgtt tctattcgca tgcacggggg
      541 ctgtgagtta caccttgggg ccgttcgggc tccgagggtt tgcctcggct ggtgtgggat
      601 ttctgattgc gttggtggtt ctgctggcgg aattgcggct gcgccgggcg gcgttgagcg
      661 ggctgctggg cggggcgttt ggcgcggtcc ttggtgtatt cgctgccctc ctagtgaccc
      721 tggtgatctc ccgcacggac gaacccgaac ccaccaagtc gttcctcgaa tttgccgcac
      781 tctttgcttt tggctatctg gggctcgtgc tcgggtcgcg ccgaggcggg gaaatgcatg
      841 ttgaggcttt ggacgggttc ttcggaaaga agtccactcc cgcggaatcc ctgaagcttc
      901 tggacaccag cgtattgatc gacggccgca tcgcggatat ctgcgatgcg cagttcctgg
      961 aaggggttct tggcgtgccg caattcgtgc tgcacgagct gcagatggtg gcggattctt
     1021 cggacccgct gaagcggcag cgcgggcggc gcgggctcga ggtgctgcaa aggattcaga
     1081 agatgccaca ggtggaagtg aggattctgg aggaggattt tccgcaggct ggggacgtgg
     1141 atcacaaact ggtggagctg gcgcggcgca ctggggcgaa aattgttacc aacgatttta
     1201 atttgaacaa ggtggccacg gtgcagggct tctcggtgct gaacgtcaac ctgctcgcgc
     1261 acgcgctgaa gcctgtcgtg cttcccggcg agcccatgcg ggtcttgatt cttcgtgaag
     1321 gcaaagaacc gaaccagggt gtggcgtatc tggatgacgg gacaatggtc gtggtggatg
     1381 gcgcgcgccg gatgatcaac aagagtgtcg atatcattgc gacctccgtt catcagacca
     1441 cggcaggcaa gatgatcttt ggtcgactgg aagaacgggc ggaacaggcg gctcctgcga
     1501 tgcggcaagc tgccgcggca ggccggggag acggcagtgg cagcgtgcgt tccgatgcta
     1561 ccagccgggt ggaaacgtct gcgcgtcttc cggagcagcc tacgctggac cgtcaggcac
     1621 gtcccaagtt tcctgaaccc gatgcaagct agtcgatagc gcgccacagc taccgatttc
     1681 accctgcgcc cccgcttgag gtacactctg aagtttgcga gtccgtgcct tgaagcgtcc
     1741 gggcggaatc gctccaaaca tgagccgaat cgctgccatt cttcccgcgg ccggtatggg
     1801 aacgcgcatg ggcgcggaaa cgcccaagca gtttctggag ttggatggaa cgccgatcgt
     1861 gatcctcagc ctgaggcgga ttgccgcttg cccgcaggtt accgacatca ttgtggcgac
     1921 gcgggcggat gaggtgggga ggctggagga acggatcaag caggaaaaat tcaagcaggc
     1981 ggtgcgcgtc gtgaagggcg gcgactcgcg gcaggattcg gtggctgcgg cgctgcgcga
     2041 ggtggcgaac gacacggaaa tcgtgctggt gcatgacgcc gtacggccct tcgtgacgcc
     2101 ggagcagatt gcgcgagtga tcgaggaagc gcggcgctgc gaggctgcga ttctgggcat
     2161 cccggcgatg gacacggtca aggaagtaaa gcgcgccagc ctgccggagg acgtggcgtt
     2221 gattacggga acggtgccgc gcgagcgcgt ggtgctggcg cagacgcctc aagcgttttc
     2281 tacgaaactt ctgaaagagg cttttgcgcg cgcggaggcc gatggcgtga atgtctcgga
     2341 tgaggcggga ctcgtcgagc gcatagggca cgacgtgcat gtcgtgctgg gctccgagcg
     2401 gaatatgaaa atcacgaagc cggcggatat ggaattggcg cgcttctacc tggaacgcga
     2461 gcgccagaag gcatgagcac gcgctgcgga atcggctacg acctgcatcg actcgaagag
     2521 ggccgcaaac ttattgttgg cgggatcgag ctgccttttg acaaagggcc ggtgggacac
     2581 tcggacggcg acgtgctggc gcatgctctt tgcgatgcgc tgctgggtgc ggcggggctg
     2641 ggcgatatcg gcacgcattt tccggacacc gatccaaagt ggaagggcgc gaacagcatg
     2701 ctcttcctgg cacatgcgaa aaagctgctg gatgaaaagc atttcgcgat cgagcatgtc
     2761 gatgcggtga tcatcacaga gaagcccaag cttgggccgc attttccgaa gatgcgcgag
     2821 gcgttggcaa aggcgctgag cgttggcgtt gaaaagattc atctcaaggc caagaccaac
     2881 gaaggcgttg acgccatcgg ccgcggcgaa gccatcgcgg cgcatgtgat tgcgacgctc
     2941 acttcccgct gaattgccga agtgtgcttg caggggcgca ggattccttc gcttgcgctc
     3001 aggataaagc tgcgccgcta cgctacaatc ctgttgatgc ttcctgccga tcattctgac
     3061 gctcctgttg ccgcagcgaa ccccttcact gccgccgacg ttctcggcat tctccgcgaa
     3121 cgcggctggc tggctgcaga gccttcgctt gagcaacagg cctggtgcga acgcgccgct
     3181 gcgatgctcg gcgggcacgc cactgaccgc gctgcgctgg gtgatctgct cggcctggtc
     3241 tttcaatacg acgcgcgcga aatcatttcc agggtagaaa gccatgtggt gctctcgcgc
     3301 tatgcggcgc gggacgtgct gcggcaggtg gcgctgctgc tcctggatgg agcgccgctg
     3361 acttcggaaa gattcaagga gattgtcacg gccttgaaag atggcatggc attgcgcgga
     3421 cgcgagctgt ttcatcccat ccggctggcg ctggccgggc gcgccgggga aggcgaactc
     3481 gaccgggtga ttctgttgct tgatgaagct gctgcagttt ccttcacgat gccggtcaaa
     3541 tcggctcggg cgagaattct ggagttttgt tcggcattgg attagttttg ccatcggata
     3601 gggcgcagca tgctgcgccc ctacaaagag atacgaaacc aaaaaccagg gcgaactgaa
     3661 gttcgcccct accacgatgg tgtgaggaag gcacgggctc acaacggaca atgaacgcct
     3721 ggctttcaag tgtagtcgct gatctgtaaa ctgtctcttt catggacaag ctgactggca
     3781 ttcacgccgt taaggaagcg ctggaagccc ggcgccccat cgatcgcatc gcgattgcga
     3841 aggggcggca ggacacgcgg gtcgaagaaa tcgtgcaact ggcgcgccag cagggcgttc
     3901 ccgtgcgttt cgaggaccgc gggcagctcg accggttggc caattcgaaa gatcatcagg
     3961 gagtggtcgc gctggcggcg gcgcgcgcgg cggcaactct ggaagatatt ctggcgcttg
     4021 ccaatgccgg ccccggccag ttagggctga tcgttctgct ggatggcgtc gaagatccgc
     4081 acaatttggg cgcgatcatt cgaacggcgc tggctgcggg cgcgcacggc gtggtgattc
     4141 ccgaacggcg cgcggcagga ctcaccgata ccgtcgcgcg cgcttccgca ggagcgctgg
     4201 cgcatctgcc ggtcgcgaag gtgaccaacc tcgtccgaac gatggaggaa ttgaaggaag
     4261 cgggctattg gctcgtgggc ctggatgagc agggggataa gaattacacc gaagtggact
     4321 acacctcacc ggtgggaatc gtcctcggga gcgaaggcca agggttgcac gagctgacgc
     4381 gcaaacggtg tgacttcgtt gtctcgctgc cgaccactgg cccggtaaaa tccctgaacg
     4441 tttccgtggc cgccggcgtc gtgctcttcg aagctattcg ccaacggaac atgcgcgcac
     4501 gagagaagta acttcatttc catgcgaact cgaaatcgcc ggatgctctt gcttacttgt
     4561 cgtactgaat gagtgagaag acatcgaggc cgttcagttt gctacggccg ttgaggaaag
     4621 tgagctcgac ggcgaaggcg gcgccttcga ccgtaccgcc gagttgctga atgagcttgg
     4681 cggtcgcggc ggctgtgccg ccggtggcga gcaggtcgtc gcagatgatc acgcgctggc
     4741 cgggcttcac ggcgtcttcg tggatttcga gcgtgtccgt gccgtattcc agggcgtagg
     4801 agatgctggc ggttttggcg gggagcttct tgggttttcg aacgggaaca aaacctgcgc
     4861 caagccggta cgccagagcg ggagcaaaaa tgaagccgcg cgcttcgatc cctactacca
     4921 cgtcaatcgt gtggccctgg tagtgctcgc agagccggtc gatcagcatg tgaaagccct
     4981 gcttgtcttt cagcagcgtg gtcaggtcgt aaaaaaggat gcccggcttc gggtagtccg
     5041 gcacttcgcg gatcagcttt ttcaagtcgt tcattcgaac gtctcctcgt tgttggcttc
     5101 aagtttgttc gcgcaattca agcgctggga ttgataacga atccgtcctc atactgcgga
     5161 tccggaacgg cggtctcttc gtgaacttcg ctgatgctgg agaaacgcgg cccgcgttcc
     5221 agcatggccc gaaattccgc gtgctgctgc ggcgcaccca tggcaaacac ttccacgcgg
     5281 ccgtcgccga ggtttctgac gaaaccgctg acgcgcaatt tttccgcggc agcctgcgtg
     5341 aaataacgaa agcccacgcc ctggaccatt cccgacacga tgtaccgctt ggctagttta
     5401 gcgtcactca cgaatcgtca aaattgtagc agagagccaa gatttcttcc ttggccgctc
     5461 catccggttc gtccagctag ggtcgcgcgc tcggagtgcg ttaaact
//
LOCUS       DASYVO010000503         8572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_69735, whole genome shotgun sequence.
ACCESSION   DASYVO010000503 DASYVO010000000
VERSION     DASYVO010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69735"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1975
                     /gene="glgB"
                     /locus_tag="VGR03_17405"
     CDS             <1..1975
                     /gene="glgB"
                     /locus_tag="VGR03_17405"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV1996114.1"
                     /translation="LPGGYRLIVKYPGGISATVHDPYNFPPMLSDFDLHLIGEGTHYQ
                     KYEKLGAHLRAVNGVQGVQFGVWAPNALRVSVVGDFNHWDGRVHPMRARGSTGLWEIF
                     LSGLEEGMIYKFEIRSRLSDLPFLKADPYGFSAELRPKTGSVVCDINRYAWHDDDWMA
                     DRATRNWLEAPISVYEVHLGSWRHARSEAGRFPTCREIADELIPYVKEMGYTHVELLP
                     VMEHPYDASWGYQTVGYFAATSRYGGPADLMELIDRFHQAGIGVILDWTPAHFPRDAH
                     GLAQFDGSHLYEHEDPRRGAHPDWGTLVFNYGRVEVQNFLIANALFWLEKYHFDGLRV
                     DAVASMLYLDYSRKAGEWVPNEYGGNENLAAVAFLKRLNEVIHGRHAGVLTIAEESTS
                     WPGVSRPTYLGGLGFSLKWNMGWMNDTLKYFKLDPVHRKFHHNQITFSMIYAFSENFV
                     LPFSHDEVVHGKASLLGKMPGDDWQKLANLRTLYGYLYAHPGKKLLFMGSELGQWSEW
                     NHDRSLDWGLLGHASHRGLAQLVADLNHLYTAEPALHQVDFDWHGFEWMDCHDSDHGV
                     LSFVRRAKDPGDFLLVVANLTPVVRENYRVPVPAPGFYREVLNTDAAAYGGTNVGNLG
                     GVPADPMAWGDHPHSLKLTLPPLAALFLKPTRA"
     gene            2065..2664
                     /locus_tag="VGR03_17410"
     CDS             2065..2664
                     /locus_tag="VGR03_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996115.1"
                     /translation="MATSKFKIAGLLAAGAVLAFAAATAAPLAAQNASTSGERWLHVS
                     VTKQNPQGERVRVNLPLSIAEGVLTSVKHEKLDHGIIKIDEVKMDQVDIRKLYATLKN
                     AKDGEYVTVESVSCNVRVAKEKGFLIVKAHDRNKKDADVYVRMPLEVVDALFSGKPDE
                     LNLVAALHVLAGRGDTELVSVKDSESTVHVWVDSKNTGD"
     gene            2740..3165
                     /locus_tag="VGR03_17415"
     CDS             2740..3165
                     /locus_tag="VGR03_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996116.1"
                     /translation="MVFGKILLGTALVGGAVIMQDGVVSVNVREKNPGGHHIWFMAPG
                     AIVPFGMKFVPAHHLKDHLRDTREWMPVARAALDALQKSPDGVFVEVDTPRKHVRIQK
                     SWGRIVVDVDDPNEEVHVAVPVRLMRHTLSELEELQPAS"
     gene            complement(3273..4055)
                     /locus_tag="VGR03_17420"
     CDS             complement(3273..4055)
                     /locus_tag="VGR03_17420"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV1996117.1"
                     /translation="MAAPADKFYRARIIERKDFAADLWMIRVDPGGEFKFTAGQYATL
                     GVETPAKVVEKPYSIVSSPYEQGIEIFVELVPEGELTPLLYKLPTGSELTMRKVPKGR
                     FILDTKSGHTRHLLLSTVTGIAPFVSYVRTLARDWKAGTFKGEHKLFLLNGASRSWEF
                     GYREEMERFAAELPWFKYVPTISRPWEDAAWRGETGRVEDLIRKYADQWECDPASTTG
                     YLCGHPQMIEHGKGILQRRGFAKESLREEIYWIPEKASGVVI"
     gene            complement(4209..4655)
                     /locus_tag="VGR03_17425"
     CDS             complement(4209..4655)
                     /locus_tag="VGR03_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996118.1"
                     /translation="MATSVLGLPQGGGTFKMLMSVNGDQTVTMRMVTRMGNDVIEMDM
                     NMPMMKNRQTPSPADARHGAERVGTETITVPAGTFACEHWRANDGTSDFWISDKVRPM
                     GLVKTVGKDSSMVLLRLITDAKTKLPPPYKKFDPMEMMRQQQQQHP"
     gene            complement(4970..5662)
                     /locus_tag="VGR03_17430"
     CDS             complement(4970..5662)
                     /locus_tag="VGR03_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996119.1"
                     /translation="MRKILFVIAALLLGGIAAWAGDPWKQKAPKEWNDDDLRQILYNS
                     PWAKEVDIVEQDAGRMAVVGGLSGHMGGSVPMPTAATSALRFIVRWASAQTTRRALVY
                     AATRSGSMNAEDAEKSLEVPIEDYVVAVLGPGLSALQKFDEAALMKMAYLKPKKANEK
                     LAPTKVVFEREADGKTVYAILFHFSKKLGNGDPTIAAGEKGMEFVCKTPGANIQTKFE
                     MEKMVAQQGTDY"
     gene            complement(5817..6905)
                     /locus_tag="VGR03_17435"
     CDS             complement(5817..6905)
                     /locus_tag="VGR03_17435"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1996120.1"
                     /translation="MAQLLWNNLRARPLRTALSVVAVALQVTFVLLIVGMSLGVVSDW
                     GKRVEGVGADVLVQPPNASIFFQFTNAVLQQSVAETIASIPGVDQVAPVLVIANARNL
                     EIVYGIDFQSFSALSQGFVFRSGRAFERPDEVILDDIQAEKHHLSVGDRLSIFSRDFT
                     VCGIVAHGKGARSFIPLATAQEIAGADRRVTLFYVRSTGDTERTRQAIVQKLPGHLVR
                     SMQDYLTLMNSSNLPGMKPFLNAMVIIGVSISFLVVLLTMHTMVLERTREIGILKALG
                     ISRMEVARLFLGETALMAALGVVLGLAATFLAEWVLKAYAPGLIILITRGWLLRSVAL
                     AFLGALLGAVYPAVRASGCDPVEALAFE"
     gene            7065..8132
                     /locus_tag="VGR03_17440"
     CDS             7065..8132
                     /locus_tag="VGR03_17440"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV1996121.1"
                     /translation="MTEPTSTSRVPIHRLLVTGISGNLGRRLAPLLEGFDLYSADLHP
                     SPPEIPAGRFHQLDLSCADGQAQIAGLIEREAIEAILHLAFVIDPLQTGIVDPKLMRR
                     ANVEATQKLLEAVAASNRRSTRVRLFVFPSSVSAYGPDLPPLVREDAPLAAHTFPYAI
                     HKRECDETCRRFHPKLGGCALYIFRPHIFAGRTVNNFILQAMRGRASGRGWLARMVER
                     RRWRVPILLPASSRGNNLFQFVHVDDVARVLSWTLKNYEPGQLRVLNLAGSGPPISFA
                     ECAAMFGTPIVHLPGEGTVTRVLKLFWNLGLSGVPPEALPYFLGSYTMDTNFLRETLG
                     ADYPSLIQWSSRDALADALKA"
     gene            complement(8310..>8572)
                     /locus_tag="VGR03_17445"
     CDS             complement(8310..>8572)
                     /locus_tag="VGR03_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996122.1"
                     /translation="GEKDQPVDNASVYVRFPEARILGREKLVEMNVKTNRDGMVKVPN
                     VPRGRALIQVIAAGWKTFGRWFELDKEEQTIKIKLEKPPRWY"
BASE COUNT         1610 a         2815 c         2389 g         1758 t
ORIGIN      
        1 ccttcccggc ggataccgcc tcatcgtcaa atatcctggc ggaatcagcg caaccgtcca
       61 cgacccgtac aattttccgc cgatgctcag cgatttcgat ctgcatttga tcggtgaagg
      121 cacgcactac cagaaatatg aaaaactcgg cgctcacctg cgcgcggtga acggtgttca
      181 gggagtgcaa ttcggtgtct gggcgcccaa tgcgctgcgc gtcagcgtgg tcggcgattt
      241 caatcattgg gacggccgcg tccatcccat gcgcgctcgc ggctcgaccg gcctctggga
      301 aatatttctc tccggtcttg aagagggaat gatttacaag tttgaaatcc gttcacgcct
      361 cagtgatctt ccgtttttga aggccgatcc ctacggattt tccgccgagc tacgccccaa
      421 gaccggctcg gttgtttgcg acatcaatcg ctacgcctgg cacgacgacg actggatggc
      481 ggatcgcgcc acgcgcaact ggctcgaggc gcccatctcc gtttacgaag tgcatctcgg
      541 ctcctggcgc catgcacgca gcgaggccgg acgcttcccc acctgccgcg agatcgctga
      601 cgagttgatt ccgtacgtca aggagatggg ctacacgcac gtggagctgc tgcccgtcat
      661 ggagcatccc tacgacgcct cctggggcta tcagaccgtc gggtacttcg ccgccaccag
      721 ccgctacggc gggcccgcgg acctgatgga gctcatcgat cgattccacc aggccggcat
      781 cggcgtgatt ctcgattgga cccccgcgca tttcccgcgc gacgcccacg gcctcgcgca
      841 gttcgacggc tcgcatctct acgagcacga agatccgcga cgcggcgcgc atcccgactg
      901 gggcacgctg gtcttcaact acggacgcgt cgaagtccag aatttcctga ttgccaacgc
      961 gctgttctgg ctcgaaaaat atcacttcga cggcctgcgc gtcgacgccg tggcctccat
     1021 gctctatctc gactactcgc gcaaggccgg cgaatgggtg cccaacgaat acggcggaaa
     1081 tgagaatctc gccgcggtcg cgtttctcaa acgattaaac gaagtgattc acggccgcca
     1141 cgccggcgtg ctgaccatcg ccgaggagtc cacttcctgg cccggcgttt cccggcccac
     1201 gtacctcggc ggcctgggat tcagcctgaa atggaatatg ggctggatga acgacacgct
     1261 gaaatatttc aagctcgatc ccgttcatcg caagttccac cacaatcaga tcactttttc
     1321 gatgatctat gccttcagcg aaaatttcgt tctgccgttt tcccacgacg aggtcgtcca
     1381 cggcaaagcc tcgctgctcg ggaagatgcc cggcgacgat tggcagaaac tggccaacct
     1441 gcgcacgctc tacggctatc tctacgcgca tccgggcaag aagctgctat tcatgggcag
     1501 cgagctcggc cagtggagcg agtggaacca tgaccgcagc ctcgactggg gtttgctcgg
     1561 ccacgcctcg caccgcggcc tggcccagct cgtcgcggac ctgaaccatc tctacaccgc
     1621 cgagccggcg ctccatcagg tggatttcga ctggcatggc ttcgagtgga tggattgcca
     1681 cgactccgac cacggggtcc tgagcttcgt ccgccgcgca aaagatcccg gcgattttct
     1741 cctggtagtc gccaacctga cgccggtcgt gcgggaaaac tatcgcgtgc ccgtgccggc
     1801 tccgggcttc tatcgcgaag tgctgaatac cgacgccgcg gcctacggcg gcaccaatgt
     1861 cggaaatctc ggcggcgttc cagccgaccc catggcctgg ggcgatcatc cgcattcgct
     1921 caagctgact ctgcctcccc tcgccgcgct cttcctcaag cccactcgcg cctgagccac
     1981 ctctcccaag gtcagttctg cgggaacttt tcccggcagg ccctcgtagt gacaggtaag
     2041 agcgaggcga ggaggcctct caacatggca acgtcgaaat tcaagatcgc aggtcttctg
     2101 gctgccggtg cggtgctggc atttgctgcc gccacggctg cgccgctggc cgcgcagaat
     2161 gccagcacct cgggcgagcg ctggctgcac gtatcagtga ccaagcagaa cccccagggt
     2221 gaacgtgtgc gcgtgaatct ccccctctcc atcgccgagg gtgtgctgac ctcggtcaaa
     2281 catgaaaagc tcgaccacgg catcatcaag atcgatgaag tcaaaatgga ccaggtggac
     2341 attcgcaagc tctatgccac gctcaagaac gccaaggacg gcgagtacgt caccgtcgag
     2401 agcgtttcct gcaatgtacg ggtggccaag gagaaaggct tcctgatcgt gaaagcgcac
     2461 gaccggaata agaaagacgc cgacgtctac gtgcgcatgc cgctcgaggt ggtcgacgcg
     2521 ctcttctccg ggaagccgga cgagctgaac ctggtggccg cgctgcacgt ccttgccggt
     2581 cgcggcgaca ccgagctggt ttcggtgaag gactcggaga gtaccgtgca cgtctgggtg
     2641 gactcgaaaa ataccggcga ttaattgctt ctctcctcac cccttcaggc gcgaagcagt
     2701 tcgcggcggc ggtcgagagg gaaacaggga ggcgaggtca tggtcttcgg aaagattctg
     2761 ctgggcacgg cgctggtcgg cggggccgta atcatgcagg acggcgtggt cagcgtgaac
     2821 gtccgcgaga agaatcccgg cggccatcac atctggttca tggccccggg cgcgatcgtg
     2881 cctttcggga tgaaattcgt tccggctcac catctgaagg accacctgcg cgacacgcgc
     2941 gagtggatgc ccgtcgcgcg cgccgccctc gacgcgctcc aaaaatctcc ggacggtgtc
     3001 tttgtcgagg tagacacgcc acgcaagcac gtacggatac aaaaatcctg gggccgcatc
     3061 gtcgtggatg ttgacgaccc caacgaggaa gttcacgtcg ccgtgcccgt gcgcttaatg
     3121 cgtcacaccc tcagcgagct cgaagagctt caacccgctt cctagaactc tcgcaacgag
     3181 ggacacttcg gaccagcggc ggcggcgctt aagaggctat ctcgccccgt ccgatgcttc
     3241 gtcacggttt ctgtggggaa ctcgccacca gctcagatga cgacgccgct cgctttctca
     3301 ggaatccagt agatctcttc ccgcagcgac tccttggcaa agccgcggcg ctgcagaatg
     3361 cctttgccgt gctcgatcat ctggggatgg ccgcacaggt agcccgtggt cgaggccggg
     3421 tcgcactccc actggtcggc gtacttgcgg atcaggtctt cgacgcggcc ggtctctccc
     3481 ctccaggcag cgtcttccca cggccggctg atggtcggca cgtatttgaa ccaggggagc
     3541 tccgcggcga agcgctccat ctcctcgcga tacccgaact cccaggaccg gctggcgccg
     3601 ttcaaaagga aaagtttgtg ctcacccttg aatgtgccgg ccttccagtc ccgcgccagc
     3661 gtccgcacat agctgacgaa cggcgcaatt ccagtaacgg tggaaagtaa aaggtgcctg
     3721 gtgtggccgc tcttggtgtc gaggatgaag cgccctttcg ggactttgcg catcgtcaat
     3781 tcactgccgg tgggcagctt gtagagcagc ggcgtgagct cgccctccgg taccagctcg
     3841 acgaaaatct ctatgccttg ctcgtacggg gaggaaacga tggagtaagg tttttcgacc
     3901 accttcgccg gtgtctcgac gcccagcgtc gcgtactgcc ccgccgtgaa cttgaactcg
     3961 ccgcctggat cgactcgaat catccacagg tcagccgcga agtcttttcg ttcgatgatc
     4021 cgggcccggt agaatttgtc cgcaggagcg gccatggtca tctcccctcc gagccagaga
     4081 cgaactgaag cgacacacct cttctctggt tcaggccatc cttcacaatt cccggagcaa
     4141 gggctccggg gctgttggtg gggagctccg gcgcctctga gatttcgaac tgtctgcccg
     4201 ccgcaagtct atgggtgttg ctgctgttgc tggcgcatca tctccatggg atcgaacttc
     4261 ttgtacggcg gcggcagctt ggtcttggcg tcggtgatca ggcgcagcag gaccatggag
     4321 ctgtccttgc ccacggtctt gaccaggccc atcggccgca ccttgtcgga aatccaaaag
     4381 tcgctcgtgc cgtcgttggc ccgccaatgc tcacaggcga acgtgccggc cggaacggta
     4441 attgtttccg tgcccacgcg ctccgcacca tggcgggcgt ctgcggggga gggggtctgg
     4501 cgattcttca tcatgggcat attcatgtcc atctcaatca cgtcgttccc catccgcgtg
     4561 accatgcgca tggttacggt ctggtccccg ttcaccgaca tcagcatctt gaatgtaccc
     4621 cctccctgcg ggagccccag cacggacgtc gccatccagt atccggtctt gccctcgaat
     4681 tcctctttgc cctgatggcc atttccatct gcatcttctt gttgtctttg ttcgtggtga
     4741 cctcataggc ggcgccctct ccgaccagcg gggcgaagac tccgccaaaa cttggcggcc
     4801 gcgcgccaaa ctgcgccggc gcggaggaag acaacattag aatcgcattg ccgtccccgc
     4861 cagagaaatt cggattcgca tggtttacct ccctgtaatt tccgtttccc gccgcttctc
     4921 cttacttccg gcgatccccg cgcgcggctc ccctgtttcc attctccccc tagtagtcgg
     4981 ttccctgctg cgcgaccatc ttctccattt caaacttcgt ttggatgttc gcgcccggcg
     5041 tcttgcagac gaattccata cccttttctc ccgcggcgat cgtcggatcc ccgttgccca
     5101 gtttcttgga gaaatgaaac aagatcgcat acacggtctt gccatcggcc tcgcgctcaa
     5161 acaccacctt cgttggcgcg agtttttcgt ttgctttctt tggcttcagg taagccattt
     5221 tcatcaacgc tgcctcatcg aacttctgca aggcggaaag gcctggcccg agaaccgcca
     5281 ccacatagtc ctcaatggga acttccagcg atttctcagc atcttccgcg ttcatactgc
     5341 cgctgcgcgt ggctgcatac accagggcgc ggcgcgtcgt ttgcgccgaa gcccatcgga
     5401 cgataaagcg caacgcggag gttgcggcgg ttggcatagg taccgaaccg cccatatggc
     5461 cggataagcc gccaaccacc gccatgcgtc cggcgtcctg ctcgacgata tcaacctcct
     5521 tagcccaggg tgaattgtaa aggatttgcc tcagatcgtc gtcgttccac tccttagggg
     5581 ccttctgctt ccaaggatcg ccggcccagg cggcgatgcc tcctaggagc aatgccgcga
     5641 tgacgaaaag gattttgcgc attttggcct ccatccgaac catctccgaa cccgcgcggg
     5701 gcgaaccggc tggctctaaa agacggcgca tgtcaatatc cggccattcc cggactcgac
     5761 ctgcgttact cttatcaaat ttgcacctga ggaaaaaggg aaatctccgc gggtcatcac
     5821 tcgaatgcca gcgcctcgac cgggtcgcag cccgaggctc gaacggcagg atacaccgct
     5881 cccagcagcg cgccgagaaa agccaatgcg acgctcctga gcagccaccc gcgtgtgatc
     5941 aggataatga ggccgggcgc ataggccttt aagacccact cggccagaaa cgtggccgcc
     6001 aaccccagca ccacgcccag cgccgccatc agcgccgttt cccccaggaa cagccgtgcc
     6061 acttccatcc gcgagatgcc cagcgctttc aagatgccga tctcgcgggt gcgctccagc
     6121 accatggtgt gcatcgtcag cagcaccacc aggaagctga tcgagacgcc gatgatcacc
     6181 atggcattca ggaacggctt catccccggc aggttcgacg agttcatcag cgtcaggtag
     6241 tcctgcatcg agcgcaccag gtggccggga agtttctgca caatggcctg ccgggtgcgc
     6301 tccgtgtctc ccgtgctacg cacgtagaac agggtcacgc gcctgtcggc cccggcaatc
     6361 tcctgtgccg tcgccaacgg tatgaaggac cgcgccccct ttccgtgggc tacgattccg
     6421 cataccgtaa agtcccggct gaaaatgctc agccgatccc ccacgctgag gtggtgcttc
     6481 tccgcctgga tgtcgtccag gatcacctcg tccggccgtt cgaaggcgcg tcccgaacgg
     6541 aacacaaaac cctggctcag ggcgctgaag ctttgaaaat caatcccgta gacgatctca
     6601 aggtttctgg cgttggcgat caccagcacc ggagcaacct ggtcgactcc cggaatggag
     6661 gcaatcgtct cggccaccga ctgctgcagg accgcgttcg taaactgaaa gaaaatcgag
     6721 gcattgggag gctgcaccag gacgtccgca cccacaccct ccacacgctt gccccaatcg
     6781 ctcaccacac ccagcgacat ccccacgata aggagcacga aggtcacctg cagcgccacg
     6841 gccaccacgc tcagcgccgt gcgcagtggc cgcgcccgca gattgttcca tagcaactgc
     6901 gccatggaga agccgttccg ctccacaaac ccctcactag aaaagaatta tcctactcac
     6961 tctagcatcg ccgccgggcg gggtcaaatc acggtggcca gtgttccacc cggctgtgct
     7021 agatttgtcc gcgccggttt ctccaaagtg agagaggaaa gcgtgtgacc gagccaacgt
     7081 cgacttcccg cgtgccgatc caccggctgc tcgttaccgg aatttccggc aacctcggcc
     7141 ggcgcctggc gccgctactc gaaggattcg acctctactc cgccgacctc cacccctctc
     7201 ctcctgaaat tcccgcgggc cgtttccacc agctcgacct ctcgtgcgcc gacgggcagg
     7261 cgcagatcgc cgggctcatt gagcgcgaag cgatcgaagc catcctccac ctcgccttcg
     7321 tcatcgatcc gctgcagacg ggcattgtcg atccaaaact catgcggcgg gcgaatgttg
     7381 aggccacgca aaaacttctc gaggctgtcg ccgccagcaa tcgccggtcc actcgcgtgc
     7441 gtctgttcgt ttttccttcc agcgtgtccg cctacggtcc cgatctgccg ccgctggtcc
     7501 gcgaggacgc tcctcttgcc gcccacacgt ttccctacgc catccacaag cgcgagtgcg
     7561 acgaaacttg ccggcgattc catccaaagc tcggaggatg cgccctttac atcttccgcc
     7621 ctcatatctt tgccggacgt accgtaaaca atttcattct ccaagccatg cgcgggcggg
     7681 cgagcggacg cggctggctg gcacggatgg tggagcgccg ccgttggcgc gttcccatcc
     7741 ttcttcccgc ctcgtcaagg gggaacaacc tgtttcaatt cgtccacgtg gacgacgttg
     7801 cgcgcgtttt gtcctggact ttgaaaaact acgaacccgg ccaactgcgt gtcctcaacc
     7861 ttgcaggctc cggccctccg atttccttcg ccgagtgcgc cgcaatgttc ggcacgccga
     7921 ttgttcatct gcccggtgag gggacggtga cacgtgtgct caaacttttt tggaatctag
     7981 gactttcggg cgtgcctccg gaagcgctgc cgtattttct tggctcctac acaatggaca
     8041 cgaatttcct tcgggaaaca ctcggtgcgg attatccgtc gttgattcag tggtcttcgc
     8101 gggacgcgct cgcggatgcg ctcaaagcct gagctggtgc aagatttcaa caatagtgtt
     8161 gaaaagtatg tggaaaaaac tttattgtgc ctgccaactc ccttccccag aagaatttct
     8221 tacggtttgc accaatgttc agcacaaact tttttcctgt ttggcgagag gaatgctctc
     8281 ggccggtttc ttcttccccg caggcactcc tagtaccagc gcggcggttt ctccagcttg
     8341 atcttgatgg tctgctcttc cttatccaat tcaaaccacc gcccgaaagt tttccatccc
     8401 gcggcgatca cctggatgag cgcccggccg cgcggcacgt tgggaacttt caccattccg
     8461 tcgcgattcg ttttgacgtt catctccacc agcttctcgc ggcccaggat tctcgcctcc
     8521 gggaagcgca cgtaaacgct ggcattgtcc accggctggt ctttctcgcc gg
//
LOCUS       DASYVO010000504         1586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_140719, whole genome shotgun sequence.
ACCESSION   DASYVO010000504 DASYVO010000000
VERSION     DASYVO010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140719"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VGR03_17450"
     CDS             complement(<1..531)
                     /locus_tag="VGR03_17450"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996123.1"
                     /translation="MKLQDSGRREFLQRVAGWGLVCAAGSYLAPSLAEGQSNPPPPPP
                     EPPDTTWTATIVPSGEPGEPLVVAGRVFAPDGQHIVAGVVVYAYNTDKDGYYSPDGKV
                     GHPRIKGYMKTDAEGRFELHTIRPGRYPGMHIPAHVHFNLWGAGYPVQWTEELRFEGD
                     SYLTEAMKNESEALGRF"
     gene            729..1352
                     /locus_tag="VGR03_17455"
     CDS             729..1352
                     /locus_tag="VGR03_17455"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEV1996124.1"
                     /translation="MRTIRIVAAIAILATLPLTTYARRKPTAGASSSNAKIYAVSKTY
                     TTLSFTATKWMVFKEEGLFQDFSGTLTYSSQDPSKCKIDVTVQAASLDTRSSGRDRVL
                     RSDDFFDVEKYPTLSFRSTRVAATGKETYDVEGDLTIHGVTKRITVPAKVIGVRVMPG
                     IGDFAGFETTFKIDRRDFGVLGSRWSGNTVAIDPTVVLHLIIGGVHE"
BASE COUNT          319 a          490 c          451 g          326 t
ORIGIN      
        1 gaacctccca agcgcctcgg actcgttttt catcgcttcc gtcaggtacg aatcgccctc
       61 gaagcgcaac tcctcggtcc actgtaccgg atagccggct ccccacaaat tgaaatgaac
      121 gtgcgcgggg atatgcattc ccgggtaacg cccgggccgg atggtgtgta actcgaagcg
      181 cccctcggcg tcggtcttca tgtagccctt gatgcgcgga tggccgacct tgccatccgg
      241 tgaatagtag ccatccttgt cggtgttgta ggcataaacg acgacgcccg cgacgatgtg
      301 ctggccgtcc ggcgcaaaga cgcggcccgc gacgacgagc ggttcgccgg gctcgccgga
      361 cggcacgatg gtagctgtcc aggttgtgtc cggcggctct ggaggcggag gaggcgggtt
      421 gctctgcccc tccgccagcg atggcgccag ataagagccc gcggcgcaca ccaatcccca
      481 tcctgctacc ctctgaagaa attcacggcg gccgctgtct tgaagtttca tgggtttgtt
      541 ccctttgctc tccaagacgg atcggcagca ccaactgtta acaatccgat tcaggaggtc
      601 gcgggtctta gcgcggctgc gttaacactt cgctggcacc gccgtctttt ctctcgagtg
      661 atgtttcagc gacgtggtgc gaatgagcca ggcacggaac aacccactct ttcctggtgg
      721 gaatgctcat gaggacgatt cgaattgtcg ccgcgatcgc aattctcgcg acgcttccgc
      781 tgaccacgta tgcaaggcgc aagccaaccg cgggtgcatc gagctccaat gcaaaaatct
      841 acgccgtgag caaaacctat acgacgctgt ccttcaccgc gacaaaatgg atggtcttca
      901 aagaagaagg attgtttcag gatttcagcg ggacgctgac ctacagctct caagatcctt
      961 caaaatgcaa aatcgacgtg accgtgcagg ccgccagcct cgacacgcgc agttcgggcc
     1021 gagatagagt gttgcgttcc gatgacttct ttgacgtcga gaaatacccc acgctttcct
     1081 ttcgcagcac gcgcgtagcc gcaacgggaa aggaaaccta cgatgtcgaa ggtgacctca
     1141 ccatccacgg tgttacgaag cgcatcaccg tgcccgcaaa ggtgatcggg gttcgcgtga
     1201 tgcccggcat cggcgacttc gccggtttcg aaaccacctt caaaatcgac cggcgcgact
     1261 tcggcgttct tggctcacgc tggagcggca acacggtggc catcgatccg acagtcgtcc
     1321 ttcacctcat catcggcggc gtccacgagt aacgcgccta acacgcttgc actactctgc
     1381 ctctctccac gcggccgcta gtccgcagaa gctcgagtcg ctttggcaac gcctctagcc
     1441 tctgtgctat cttcaaagct cctcatgacg gcaggtagag aaatctgtct gggctccttg
     1501 cgcctcggtg gcggtaatcc cctgtttctg atcgcggggc cgtgcgtgat tgaaagcgaa
     1561 gcgcacgcgc gaatgatggc ggagaa
//
LOCUS       DASYVO010000505         4177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_140734, whole genome shotgun sequence.
ACCESSION   DASYVO010000505 DASYVO010000000
VERSION     DASYVO010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140734"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VGR03_17460"
     CDS             complement(<1..192)
                     /locus_tag="VGR03_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996125.1"
                     /translation="MNRRPRHFRHFAAQHFIRAFFLLLFAVTFACGGSAVRARPQQTA
                     SEAAIAVRKVEPPSWWVGLT"
     gene            complement(261..914)
                     /locus_tag="VGR03_17465"
     CDS             complement(261..914)
                     /locus_tag="VGR03_17465"
                     /inference="COORDINATES: protein motif:HMM:NF027338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HEV1996126.1"
                     /translation="MSKARIAVAAQVLELPDADGFPTLSSWELSVPLRFSADWQGKNV
                     DPERETEVRLLWTPESLFLRFRAKYRVMAVFPDAAPNGRRDHLWDRDVVEVFLQPDPS
                     HPRRYKEFEVSPNGFWIDLDIAPGEKHDLKSGLRRRVILDEAAKTWVAELALPMKCLV
                     DRFDPAATWRVNFYRVEGTAEPRFYSAWQPTRTPAPNFHVPEAFGELVFAPGPIPRE"
     gene            1196..2020
                     /locus_tag="VGR03_17470"
     CDS             1196..2020
                     /locus_tag="VGR03_17470"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV1996127.1"
                     /translation="MIEPVNTQDFTSQPERRKPEQFEYVKFRIDAGVARMTLNRPEHN
                     LLNEAMLREIAGGITHAGERDDVKLIVLDSACKVFCGGIDIGEYTSQRVFQMLDAFHA
                     AFAGMLEVGKPVVCVVNGPAIGGGAELAAFGDLVIATPRARFAQPEISIGIFPPLAST
                     ILPFLVGPKIALELVLTGEPVTAERALELGMVNRLVPEAQLEQAVKDLVDRISGHSGP
                     VLTMAKKAILGGMGLSLREGLKHSMNIFLNELYRLEDSQEGLRALVEKRKPNWKNR"
     regulatory      2075..2227
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2320..3402
                     /locus_tag="VGR03_17475"
     CDS             2320..3402
                     /locus_tag="VGR03_17475"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEV1996128.1"
                     /translation="MKEFAHPRLRSVVFAASAALLSCAALSRAQSAPPISSASPAFRE
                     VTDEAGRTVRIPQPVHRIVSLAPSLTETVYALGLQDHLVGDTDYCDYPADAQKKTKVG
                     SAINPSLEQIAALHPDIVLVTKGFNRLETVNSLGGLGISSYATDPHTVDEIISSSKKL
                     ADLLGAPEAGLSVADDMQRHLADLQQRLGALPPKRVLFIVWTQPLISVGKATFIADAL
                     RRAGAVSIVDSDQSWPQVSLEEVARLQPDFLVFAASHSESASPAVEVLATLPGWSIVD
                     AVTKRRFAVISDAVNRPAPRIVAAIEDLARQLHPDAFVEKPENSKEKIEKENPPPNQR
                     PASHDSYAPAALQGYVTTPGGCACAH"
     gene            3390..>4177
                     /locus_tag="VGR03_17480"
     CDS             3390..>4177
                     /locus_tag="VGR03_17480"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1996129.1"
                     /translation="MRPLTLRRLILQCSILVAILFVVVVVALKFGAVPVSLYALGRDL
                     LRFLLGQSSQISTDYGFIIWNIRGPRILLGIIVGASLSVAGTSFQALLRNPLADPYVL
                     GVSSGAALGSILALIAEPHLTISPALAALLTPLGAFLGAAATVAAVYFLGRREGQIDS
                     STLLLGGVITASFLSAVIMFLMSTLSGNLRGLAFWLMGDLSTSLQKSVYWFLGIGFLA
                     AAGAIYTTASDLNLLLAGEKEAMHLGVDVPRVRLVVYIAASLLTG"
BASE COUNT          820 a         1274 c         1187 g          896 t
ORIGIN      
        1 cgtgagtccc acccaccagc tcggaggctc gacttttctg acggcaattg cagcttcgct
       61 ggccgtctgc tgcggacgag cccgcaccgc cgagccgccg caagcgaatg taacggcaaa
      121 cagaagcagg aaaaaagcgc ggatgaaatg ctgcgccgca aaatggcgaa agtgccgggg
      181 ccgtctattc acgaggagag cataaggaaa aaaatggagg gatgccagtc tcgatccgcc
      241 gtaccagctc ggccagtccg ctactctcgc ggtatagggc ccggcgcaaa caccaattcg
      301 ccaaacgctt ccggaacgtg gaaattaggc gctggggttc gcgtgggctg ccatgcggaa
      361 taaaatcgcg gttcggcagt tccttcgacg cggtaaaaat tcactctcca ggtggccgca
      421 ggatcgaaac gatcaacgag gcatttcatc ggcagcgcaa gctccgcgac ccaggttttg
      481 gcggcttcgt ccaaaataac gcggcgccgc aaaccgctct tcaagtcgtg cttctcgccc
      541 ggtgcgatgt ccagatcgat ccagaagccg ttcgggctga cctcgaattc cttgtaacgc
      601 cgggggtgcg acggatcggg ctgaagaaaa acttccacga cgtcgcggtc ccagagatgg
      661 tcgcgtcggc cgttgggcgc ggcgtcggga aaaacggcca tgacgcggta tttcgcgcgg
      721 aatcgcaaaa aaagcgactc cggagtccag agtaatcgaa cttccgtctc tctttcggga
      781 tcaacattct tgccttgcca gtccgcactg aaacgcaggg gcactgaaag ctcccaggaa
      841 gacagggttg gaaatccatc ggcgtcaggt agttcgagca cttgagcagc caccgcaatg
      901 cgcgctttgg acatactctc gagccttctc cgggagccca acttcgacgt aactcccaga
      961 ataaccattg ccgggagatg cacaagaaaa aactgggtaa ccgagcgaac gttttttccg
     1021 ttgcaacagc tccagcgcct ttctcgaaaa ccctgttcgc ctgcctgcga actatttgcg
     1081 caccatgcat tccactcatc gtggatacca cagaacaccg tgctagcatc agatttggct
     1141 gctttctacg attttctcat gtcagaaggg caatggacgc ccaggagcgc gggaaatgat
     1201 tgagcctgtc aacacccagg atttcacatc gcaaccggaa cggcgcaaac cggaacaatt
     1261 tgaatatgtg aagttccgca tcgacgccgg cgttgcgagg atgaccctca accgcccgga
     1321 acataatctt ctcaatgaag ccatgttgcg cgaaatcgcc ggcggaatca ctcatgccgg
     1381 cgagcgcgat gacgtcaagc tgatcgttct ggattcggct tgcaaagtgt tctgtggcgg
     1441 aatcgatatc ggtgaataca cctcgcaacg cgtgtttcag atgctcgacg cgtttcacgc
     1501 ggcgttcgcg ggaatgctgg aagttggcaa gcccgtcgtt tgcgtagtga acggaccggc
     1561 gatcggcggc ggcgcggagc tggccgcttt tggcgatctc gtgattgcta cgcccagagc
     1621 gcgatttgcg cagccggaaa tcagcatcgg catcttcccg ccgctggcat ccacaattct
     1681 gcctttcctc gtcggaccca agatcgcgct tgagcttgtt ctgacgggtg aaccggtgac
     1741 ggcggagcgc gccttggaac tcggcatggt gaatcgcctc gtgccggaag ctcaactcga
     1801 acaagcggtg aaagatctcg tcgacagaat cagcggccac tcgggtccgg tactgaccat
     1861 ggcgaagaaa gcgatcctcg gcggcatggg cttgtcgctg cgcgagggcc tgaagcattc
     1921 gatgaacatt ttcttgaatg agctttaccg cctcgaggat tcgcaggaag gtttgcgcgc
     1981 actggtggaa aagcgcaagc ccaactggaa gaaccgctga acgttcgccg cgttttttta
     2041 gtcgcgccat gttccttcgt gccgttttca gtagtgcatt gacactcctg cgggcgcggc
     2101 atacactcaa atttcaattt gcaggcaagg gcagcgggga aggccgtgtg aatcggccgc
     2161 ggtcccgcca ctgtgatcgg ctgaaaagcc gtaagccagg ccaccctccc gtgccgcaga
     2221 gagcccgctc ttcgcgagaa agagcgttgc ttgaagcccc aaggtgtctc gtggggcttt
     2281 tttgttggtg cagtgaaatt tcctggtcaa gggagattcg tgaaggaatt cgcacacccg
     2341 cgcctccgaa gtgttgtctt cgccgcaagc gctgccttgc tgtcctgcgc ggcgctgtcg
     2401 cgtgcgcaga gcgctcctcc gatctcttcc gcatctccag ctttccggga agttacggac
     2461 gaagccggcc gcactgttcg cattccccag ccggtccacc gcatcgtgtc gctcgcgccg
     2521 agtttgacgg aaaccgtcta cgcgctcggc ttgcaggatc atcttgtggg tgacaccgac
     2581 tactgcgatt atccggcaga cgcacagaaa aagaccaaag tcggcagcgc aatcaatcca
     2641 agtttggaac agattgccgc tttgcatccg gatatcgttc tggtgaccaa aggcttcaac
     2701 cgtctcgaga ctgtaaattc gctcggcggc ctgggaattt cttcgtacgc cactgatcct
     2761 cataccgtcg acgaaatcat ctcttcctcg aagaagctgg ccgacttact cggtgcccct
     2821 gaagcaggat tgtctgtcgc cgacgacatg caacgccacc tcgccgattt gcaacagcgc
     2881 cttggtgcgt tgccgcccaa gcgcgttctc ttcattgttt ggacccagcc gctcatctcc
     2941 gttggcaagg ccacttttat cgccgacgcc ttgcgccgcg cgggagccgt ctccatcgtg
     3001 gattccgacc aaagctggcc acaagtcagt ctcgaagaag tcgcccggtt gcagcccgac
     3061 ttcctggttt tcgcggcttc gcattccgaa tccgcttccc ccgccgtcga agtgctcgcc
     3121 acgcttcccg gatggagcat cgtggatgcc gtcaccaaac gccgctttgc ggtgatcagc
     3181 gatgccgtaa atcggcccgc cccgcgaatc gtcgcggcca tcgaagatct cgcccggcag
     3241 ttgcacccgg acgcctttgt tgaaaaacca gagaacagta aggaaaagat cgagaaggag
     3301 aatcccccgc cgaaccagcg gcccgcgtcg catgattctt atgctcccgc tgccctgcag
     3361 ggctacgtga cgactcctgg aggctgcgca tgcgcccact gacgctccgg cgcctcatcc
     3421 tccaatgttc gatcctggtg gcgattttat tcgtggtcgt ggtcgtggcc ctgaagttcg
     3481 gcgcggtgcc cgtttcgctc tacgccctcg ggcgtgactt gctgcgtttc ctcctcgggc
     3541 agagtagcca aatctccact gactacggct ttatcatctg gaatatccgg gggcctcgca
     3601 ttcttctcgg catcattgtt ggcgcgtcgc tctccgtcgc tggaaccagc tttcaagcgc
     3661 tgctgcgcaa tccgctggct gatccctacg tgctcggcgt ctccagcgga gcagcgctgg
     3721 gctcgattct tgccctgatc gcggaacctc atttgacgat ttcgcccgcg ctcgccgcgc
     3781 tgctcacgcc attgggtgct tttctcggag cggccgcaac cgtcgccgcg gtctactttc
     3841 tcggccgccg cgaagggcag atcgacagca gcaccttgct tctcggtggt gtcatcaccg
     3901 cctcctttct ctcagccgtc atcatgttcc tgatgagcac tctctccggc aatctccgcg
     3961 gcctggcctt ttggctgatg ggcgacctct ccacttcttt gcagaaaagc gtttactggt
     4021 ttctgggcat cgggttcctt gcggcggccg gagcgattta caccacggca tcggatttga
     4081 atttgctcct cgccggagaa aaagaggcca tgcatcttgg cgtggacgtg ccgcgtgtgc
     4141 ggctggtcgt ctacattgcc gcgtctttgc tgaccgg
//
LOCUS       DASYVO010000506        14834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_222120, whole genome shotgun sequence.
ACCESSION   DASYVO010000506 DASYVO010000000
VERSION     DASYVO010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 14834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14834
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222120"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..2870
                     /locus_tag="VGR03_17485"
     CDS             213..2870
                     /locus_tag="VGR03_17485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1996130.1"
                     /translation="MSWVSRLFGKRNREEELEEEVRSHLKMAAQERAERGEAAEEAER
                     AARRVFGNVGLVKEVTREMWGWGSLDRLMQDLRFGVRLIAKSPGFTAVAILTLALGIG
                     ANTALFSVVNGVLLNPLPYPKPEQLVTLAESKPNFDSGSISFPNFRDWRKDNRTFSRM
                     GIARGHSFNLTGTGDAEQVRAEFVSSDFLPLLGVKPVIGRLFSEGEDEIGASPIVLIS
                     AEFWSRKFGWGSDVLGKGITLDGRSYTIVGVVPASFKLQVWSFRPSELYVPIGQWTNN
                     LLPSRAAGLGFHGVGRLKPGVTIQQARADMQRVTEHLTEAYASADTGIGATLMPLKEH
                     MVGGMRRLLLVLLAAVGFVLLIACVNVANLLMVRSAGRAREFAVRAALGAGRGRIVRQ
                     LLTESIVLALAGGGLGLLLAAWGTKVALQHLPSALPRASEVGLDLRVLVFTMGIALLC
                     GILFGLAPALRISRPNLQDTLKEGGRGSGGGKYRAQGVFVGAEMAMALVLLIAAGLMI
                     RSLNALWSVNPGFDAHNVLTFGVSLSPTMKDASADAIRAALREIQGKLESTPGVKAAS
                     LSWAAVPLASDDEDLFWLEGQPKPASENDMSWTISYVVQEDYLRVMGIPLERGRFFTA
                     QDNERTAHVVVIDDVFARKFFGDQDPVGRRVNLSNKGGAAEIVGVVGHVKQWGLDTDD
                     QESLRAQLYFPYMQLPDEAMRQSAGGTGVLLKLDGNMQGTAATLRTVLKEMNSEQVMF
                     GVQTMEEIIAESLAARRVSMMVLSVFAVLALGLASMGIYGVISYLVGQRTHEIGIRIA
                     LGAKQGDVLRMVLSEGMKMVVFGIVIGLLAAAGLTRLMANLLFGVSATDPLTFAGVGM
                     ILTIVALAACYVPARRAMRVDPIVALRYE"
     gene            3082..3738
                     /locus_tag="VGR03_17490"
     CDS             3082..3738
                     /locus_tag="VGR03_17490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV1996131.1"
                     /translation="MNRMQQSVESTQAIAESDAIAVERTLAGDCDAYRVLVERHSNTV
                     FRLAYRMTGNRHDAEEVVQEAFLRGYQKLGQFAARSNFGTWVYRIAANYAIDRMRQRQ
                     KEEARRDTPRAEDGDGMENDPVNRVKDEAPTPERLTQSLELRKQMEQALAALSEAERT
                     AFVMRHWEGCGIEEIAAVLKSSSNATKNTVFRAVQKLRHALQPFVETRGQARAMGTES
                     "
     gene            3735..4526
                     /locus_tag="VGR03_17495"
     CDS             3735..4526
                     /locus_tag="VGR03_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996132.1"
                     /translation="MKHMTGEELIAYREGVAEQRALIAEHLAACEECREELERIEAVL
                     AALDTLPVPDPGADYGRQVWREIAPRLAEKPGRRWQVWREPRRLAAAGALVALIIAAF
                     VLGRMMPRHGVSDNIATEEQVRERVLVVAVGGHLGRSEMMLMELSNAGPANPAQKQVN
                     ISAEQRRAEDLLEENRLYRQTALQEGDAGIAGLLDELERVLLDVAHSPEEVTPAQLEA
                     IQEKIEGRGILFKVRVVNKELQQRQEATKPAPVQNESTKRERNKV"
     gene            4556..5896
                     /locus_tag="VGR03_17500"
     CDS             4556..5896
                     /locus_tag="VGR03_17500"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEV1996133.1"
                     /translation="MAWIAIAALLLAPAVARAHGLKAIPVQSADSLQDPQEVQDKEQE
                     KRDREQEARDREEEKRDREQEARERAQEKIERLQELYDDGREDLDEDRYDGAASKFKQ
                     LAELNGPQTDAALYWKAYAENRLGRRDTALATIADLKRRFPQSRWQKDAGALEIEVKQ
                     STGQPAKPADQSDEELKMLAIQGLMNSDPERAMPLLETVLNGSASPKEKSKALFVLAQ
                     GGSQQGREIIGRIARGQSNPELQRKAVEYLGLFGGAEARKTLADVYASSGDASVKHAI
                     LRSYMIGGDHERLFAAAKSEKDESLRREAIRQLGLVRGTSELEQLYQMETSPDVRREI
                     LQALFLAGDSGRLVQAAQGEKDAELRRTAIRNLGLIHSDDSGKALQEIYSKETNREVK
                     AEVLNAYFLQGNAKALVAIARSEKDPELKKTAVSKLTLMHSKEGTDYLMELLQK"
     gene            5908..6939
                     /locus_tag="VGR03_17505"
     CDS             5908..6939
                     /locus_tag="VGR03_17505"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEV1996134.1"
                     /translation="MREERTDSALRRVFGLAMIVAGIGGAAVQLAKGGAPARLEQTSP
                     AAAQASEAPRIENAKLETRAAGASLDATMREIAATAEKAEWVGYHVDQVAGERGVCCN
                     NSWNDGNCGTCRLEKENGETSGTTHTDGNVKLEGTRQLVVLYRLEAKQVVKIRVASQD
                     CTLDAGGLAFVWLTGVKATESVALLATFVRGSEFEDHGDRGLGNGALTAIVLHAGASA
                     DRALESFVKPEQREGLRRQAAFWMGAARGKAGLTALQQMAKSDPSPDVRAHVAFALSV
                     SHEAGALDEMIRMAHDDTSSHVRGQALFWLAQEAGKKAVGTISGAIENDPDTDVKNKA
                     VFALSQLPR"
     gene            6946..7074
                     /locus_tag="VGR03_17510"
     CDS             6946..7074
                     /locus_tag="VGR03_17510"
                     /inference="COORDINATES: protein motif:HMM:NF015114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEV1996135.1"
                     /translation="MPKLIEVAQTNRNPEVRKQAMFWLGQSNDPRALQFFEKVLTQ"
     gene            complement(7079..7918)
                     /locus_tag="VGR03_17515"
     CDS             complement(7079..7918)
                     /locus_tag="VGR03_17515"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:NF008808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEV1996136.1"
                     /translation="MLPSQVERLFDEVPAHYEEEHQWLFRDFKAALNRGEIRAAEPDA
                     STKSGWRANAWVKKGILLGFRMGAIADMSIDAAKQPFFDKSTYPVHAFTSSDGVRIVP
                     GGSSIRDGSYIGRGVICMPPMFVNVGAYVGEGTMIDSHALVGSCAQVGRNCHISAGSQ
                     IGGVLEPVGALPVIIEDEVLVGGNSGVYEGTVVKRRAVLGTGTILNRSIPVYDLVRDK
                     IYTATETEPLIIPEEAIVVPGSRAVSHPVGAKWELSLQAAVIVKYRDAKTDARIQLED
                     LLR"
     gene            complement(7899..8828)
                     /gene="dapA"
                     /locus_tag="VGR03_17520"
     CDS             complement(7899..8828)
                     /gene="dapA"
                     /locus_tag="VGR03_17520"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV1996137.1"
                     /translation="MFTGTGTALVTPFRRDGSFDEPALRALIKRQIDAGIDFLVPCGT
                     TGESPTLTPEEHLRVVQITVELAKGKVSVLAGAGGYNTAEVIALARELAAIGADGILS
                     VTPYYNKPTQEGLFQHYRAIAEAVSLPIILYSVQGRTGVNIEPATVKRLAEIENIVGI
                     KEASGNVSQMVAILNAVPDDFIVLSGDDAITLPVISLGGRGVISVVSNEIPAEMSQLT
                     RLALQGDFPSARAIHRRYHPLMEINFVESNPIPVKAAMAEMGLLEPVWRLPLVPPKAE
                     NQARIRAVLESLGLVDRVAAAPGTERVHAAIAS"
     gene            complement(8853..9554)
                     /locus_tag="VGR03_17525"
     CDS             complement(8853..9554)
                     /locus_tag="VGR03_17525"
                     /inference="COORDINATES: protein motif:HMM:NF017024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEV1996138.1"
                     /translation="MSLGLAIVGYGKMGHLIEQFAPEYGFDVRAKFDGSNNVHAQALS
                     HETLRGVDVAVEFTTPQAAPQNIRRLAVLGINTIVGTTGWFDDLPSAREAVTKSGTGL
                     VWAPNFSVGVNLFMQAVAHTAALFAKHADYEAWGWEIHHSAKKDAPSGTLKKLAEEMR
                     ANGYNRPVSVGSNRAGAHPGTHEIGFDSSADTITLRHTARNREGFARGALRAARWVVG
                     KKGLFEFHEILGELG"
     gene            complement(9551..10573)
                     /locus_tag="VGR03_17530"
     CDS             complement(9551..10573)
                     /locus_tag="VGR03_17530"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1996139.1"
                     /translation="MNLFELTRALVDIESTTNHEKNVGDYLFAHLSALAAQHDGRIER
                     ISAEPNRDNIFATWGEPVVTLSTHMDTVPPFFASHEDGEFIWGRGSCDAKGIIAAMIA
                     AAEKLLAAGTRNLGLLFVVGEERNSAGAKAAAATPRGSRYLINGEPTENRLALGSKGA
                     LRYEIITQGKLAHSAYPELGHSAIHALLDVLHDIRQIPLPQDALLGRSTLNVGTIDGG
                     RAPNVVADHAEAEIMFRTVGDPAMIREAVSAAAAGRAEAREVFYTPALQLAKMDGLPT
                     TVVAFTTDIPSFESAWGQPFLIGPGSIHVAHTGEERISKKELSEAVEIYARMATQLLA
                     TGRSSA"
     regulatory      complement(10643..10863)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            11254..11742
                     /locus_tag="VGR03_17535"
     CDS             11254..11742
                     /locus_tag="VGR03_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996140.1"
                     /translation="MKLEETIRILYDASVEFVVIGGAAMGLQGSAHLTRDIGSCYART
                     PKNMERLAKALEPFHPVLRGVPPNLPFRFDAKTIAYGLNFTLSTDLGDLDFLGEVSGL
                     GSFQEVLAASDVRDVGGVDCRVLSLEGLIKSKTAAGRPRDMYVLPELRGLNEFKKKTG
                     LE"
     gene            complement(11800..12603)
                     /locus_tag="VGR03_17540"
     CDS             complement(11800..12603)
                     /locus_tag="VGR03_17540"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1996141.1"
                     /translation="MRVSILASGSSGNITLLETGRTRLLIDAGLGKRETLARLAAVER
                     SVEHVDGILITHEHTDHCNGLPQMLGLWKAPLYVTEPTMDAMQRILPETFGKRLNRVE
                     TIHPGQHFNVGDIDVHAFAIPHDAADPIGFTFRSNGAKMAIVTDLGYMPELVKVHLRG
                     ADCLVLESNHDLDMLKVGPYPWVVKQRVLSRTGHLSNHAVSEYLCDPDGFDAIARYLV
                     LAHVSQENNNPDLVRISAEEALRRRPAESAFTGELLVASQHVPLKPLEL"
     gene            12722..14110
                     /gene="sthA"
                     /locus_tag="VGR03_17545"
     CDS             12722..14110
                     /gene="sthA"
                     /locus_tag="VGR03_17545"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HEV1996142.1"
                     /translation="MEKYDLIVVGSGPGGQRAAIQAAKFGKRPALIEKLEVIGGTAIN
                     TGTIPSKTIREAVLHLSGYQYQSIYGVNYRVKEQITMADLSFRALNVIKTEIDVIRAQ
                     LSRNGIEVVTGTASFVDPHHMQVTSSRGQSEFEADNIVIATGTKPAVSPLVPFNNTTI
                     INSDQILRMPVIPKTMIVVGGGVIGVEYVCMFATLGVRVTLIEKRPRLLEFADAEMVE
                     ALCYHLRDRRVTLRLNEEVESVEETPDHTVVANLKSKKKVTGEALLYAVGRQGNVDEL
                     NLAAGGLEADARGRIPVDENYRTKQPHIFAVGDVIGFPSLGSVSMEQGRIAAARAFGM
                     PTNSNRANYPYGIYTIPEISFIGKTEEQLTEEGVPYEVGMAFFREIARGQIRGDTTGR
                     LKLIFDPSTKELLGVHIFGEGASELLHIGQAVFALKGTVEYFVDTVFNYPTLAECYKA
                     AAFNGLNKLVRA"
     gene            14173..>14834
                     /locus_tag="VGR03_17550"
     CDS             14173..>14834
                     /locus_tag="VGR03_17550"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1996143.1"
                     /translation="MRRTVGVLVMEHIAVGLVEGDKVVGPLNIFPEKPQTLDPLQSMP
                     AESIVECIRQQVEKVADGEKIAAIGIGFPGIIRSGVVEDSPNLQQVKGFALQAALSSA
                     LGGKAAGVPVRLFNDADVMAAGIAATQGKLNQLTRVWTLGNGVGFGRYPSSEGIWEGG
                     HSVVTLDPKENFCGCGGRGHLEGILGRRSMRMRFLDLEPEEVFENARAGDARCAEFVT
                     LC"
BASE COUNT         3272 a         3802 c         4904 g         2856 t
ORIGIN      
        1 tgcgggtctt atcgggcgaa ttgtacggcg tgggtgcgac agatccgttg acgttttgcg
       61 gcgtggcgat cctcgttgct gcggtggcat tgaccgcatg ttatgtaccg gcacggcggg
      121 cgatgcgggt ggatccgatg gtggcgctga ggtacgaata gaaattagaa aatggaaatc
      181 agaaattaga aattggaaga agaggtagcg agatgagctg ggtgagccga ctttttggca
      241 aacgaaaccg ggaagaagaa ttggaggaag aagtgcgaag ccatctgaag atggcggcgc
      301 aggagcgcgc agagaggggc gaggccgcgg aggaagcgga acgtgcggcg cggcgggtgt
      361 ttgggaatgt gggattggtg aaggaggtca cgcgggagat gtggggctgg ggatcacttg
      421 accggctgat gcaggacctg cgatttggcg tgcggctgat cgcgaagtcg ccggggttta
      481 cagcggtggc gatcctgacg ctggcgctgg gaattggcgc gaacacggcg ctattttcgg
      541 ttgtgaacgg agtgctattg aatccgctgc cctatccgaa gccggaacag ctcgtgacac
      601 tggccgagag caaaccgaat tttgactcgg ggtcaatctc cttcccgaac tttcgagact
      661 ggcgtaagga taaccgcacc ttttccagga tgggcattgc acggggacac tcattcaacc
      721 tgacggggac gggcgacgcg gagcaggtca gagcggaatt cgtttcgtca gacttcttgc
      781 cattgctggg cgtgaagccc gtcatcggac gattgttttc tgagggagaa gacgaaatcg
      841 gcgcgagccc catcgtgctc atcagcgcgg agttctggag tcgcaagttt ggctgggggt
      901 cggacgttct gggaaaagga atcacgctcg atgggcgaag ctacacgatc gtcggcgtgg
      961 tccctgccag tttcaaattg caggtttgga gcttccgccc aagcgaactc tatgtgccca
     1021 tcggccagtg gaccaataat ctgcttccaa gccgggcggc tggactgggg tttcacggag
     1081 ttggccggct gaagccgggc gtgacgattc aacaggcgcg ggcagacatg caacgagtga
     1141 cggaacactt gacggaagcg tatgccagcg cggacacggg aatcggcgcc acacttatgc
     1201 cgctcaagga acacatggta gggggcatgc gccggttgct gctggtgctg ctcgcggccg
     1261 ttgggtttgt gctcttgatc gcttgcgtga acgtggccaa tctgctgatg gtccgctcgg
     1321 cggggcgcgc aagagagttt gccgtacgtg ccgctctcgg tgccgggcgc gggagaatcg
     1381 tgcgccagct tctaacggaa agcatcgtgc tggcgctggc tggtggaggg ctcggcctgc
     1441 ttctggcggc gtggggcacg aaggtggcgc tgcaacatct gccgtcggca ttgccgcgcg
     1501 ctagcgaggt cggcctggat ttgcgcgtat tagtattcac aatgggcatc gcgctgctct
     1561 gcggaattct ttttggattg gcgccagcgc taaggatatc gagaccgaac ttgcaggaca
     1621 cgctgaaaga aggaggccgg ggctcgggag gcgggaaata ccgcgcgcag ggagtgtttg
     1681 ttggtgcgga aatggcgatg gcgctggtgt tgctgatcgc ggcgggactg atgatccgca
     1741 gtctgaacgc gctctggagc gtgaatccgg gcttcgatgc gcacaacgta ttgacgtttg
     1801 gtgtttcgct ctcgccgacc atgaaggacg cgagcgcgga cgccattcgc gcggcgctgc
     1861 gggaaatcca aggcaagctg gaatcgactc cgggagtgaa agctgcttcg ctttcgtggg
     1921 cggcagtgcc gctggccagc gatgacgagg atctcttctg gctcgaaggt cagccaaagc
     1981 cagccagcga gaacgacatg agctggacga tcagctacgt ggttcaagaa gactatctaa
     2041 gggtaatggg aattccgttg gagcgcggac ggtttttcac agcgcaagac aatgaacgca
     2101 cggcgcatgt ggttgtgata gatgacgtct ttgcgcgaaa gttctttggg gatcaagatc
     2161 cggtcggaag acgcgtgaat ctaagtaaca agggaggcgc ggcggaaatc gttggggtcg
     2221 tcgggcatgt aaagcagtgg ggactcgaca cggatgatca agaatcgctg cgcgcgcagc
     2281 tctattttcc gtacatgcag cttccggacg aggccatgcg ccagtcggcg ggcggcacgg
     2341 gagtgctgct gaagctcgac ggaaacatgc aaggaaccgc cgcgacactt cggacggtct
     2401 tgaaagaaat gaacagtgaa caggtgatgt ttggagtgca gacgatggag gagatcattg
     2461 cggagtcgct ggcagcgcga cgggtctcga tgatggtgct gagcgtcttt gctgtgttgg
     2521 ctctggggct ggcgagcatg ggaatttatg gcgtgatttc ctacctggtt ggccagcgga
     2581 cgcatgagat tggcatccgc atcgcgcttg gcgcaaaaca gggcgacgta ctacgcatgg
     2641 ttttgagcga gggtatgaag atggtcgtgt ttggaattgt gattggcttg ctcgctgcgg
     2701 cggggctgac tcgcttgatg gctaatttac ttttcggggt gagcgccacc gatccgctga
     2761 cgtttgcggg cgtgggaatg atactgacta tcgtggcgtt ggcggcgtgt tacgtgccag
     2821 caagaagagc gatgcgagtg gacccaattg tggcgctgag atatgagtga aaaaattagt
     2881 taaagaagta acgaagtaaa gattgcagag tgcgcgaaag agcgatgcgt tgccttacag
     2941 gagcgcagta gccttcgact tcgctcgggc caagtgctgt acccctcctt ccagtgatat
     3001 cccgggggac caaagaagcg ctatgttgtt gatggataag gccctaagcc cgacctaacc
     3061 gcaagcctcg aatcctgtct aatgaatcgg atgcaacaga gcgtcgaaag cacccaggcc
     3121 atcgcagaga gcgacgccat cgccgtggag cgcacgctcg cgggcgattg cgatgcctat
     3181 cgcgttcttg ttgagcggca cagcaacacc gtttttcggc tggcctaccg gatgaccgga
     3241 aaccggcacg atgccgaaga agtggttcag gaggcattct tgcgcgggta tcaaaagctg
     3301 gggcagttcg cggcgcgatc gaatttcggg acgtgggtct atcgcatcgc ggcgaactat
     3361 gcgattgacc gcatgcgcca gcggcagaag gaagaagcgc ggcgggatac gccgagagcg
     3421 gaagacgggg acggaatgga aaacgatccg gtgaatcgcg tcaaagatga agccccaacg
     3481 ccggaacggt taacgcagag ccttgaactg cgaaaacaga tggaacaggc gctcgccgct
     3541 ctttcggaag cggaaagaac cgctttcgtg atgcgccatt gggaaggctg cgggatcgaa
     3601 gaaatcgcag cagtcttgaa atcaagttcg aatgcgacga agaacaccgt ctttcgagcg
     3661 gtgcagaaat tgcggcatgc gctgcaaccc tttgtggaaa caagagggca ggcacgcgcg
     3721 atgggaacgg aatcatgaaa cacatgaccg gagaagagtt gattgcgtat cgcgagggag
     3781 ttgccgagca gcgagcgctg attgcggagc atctggcggc ctgcgaagaa tgccgcgagg
     3841 agttggaacg aatcgaagcc gtactcgcgg cgttggatac gttgcctgtg ccggatccag
     3901 gggcagatta cgggcggcag gtgtggcgag agattgcacc gcggctggcg gagaaaccgg
     3961 gacggcggtg gcaggtgtgg agggagccac ggcggctcgc ggcggcgggc gcgcttgtgg
     4021 cgctgattat cgccgcgttt gttctaggac gaatgatgcc gagacacgga gtgagtgaca
     4081 acatcgcgac cgaggaacag gttcgagagc gcgtgttggt agttgccgtg ggcggacacc
     4141 tgggccgttc cgagatgatg ctgatggagc tttcgaacgc cgggccggca aatccggcgc
     4201 agaagcaagt gaacatctca gccgagcagc ggcgcgcaga agatttactg gaagaaaacc
     4261 gtttgtatcg ccagacggcg ctgcaagaag gtgatgccgg gatcgccggc ttgctggacg
     4321 aactggagcg cgtgctcctg gacgtggctc acagcccgga agaggtaacg ccggcgcagc
     4381 tcgaagcgat ccaagaaaag atcgaggggc gcggaatttt gttcaaagtc cgggtggtca
     4441 acaaggaact gcaacaacgg caggaggcca cgaagcctgc gccggtgcag aacgaatcga
     4501 cgaagagaga gaggaataaa gtatgaccat cggcaattca gttggaaaga caagtatggc
     4561 ttggatcgca atcgctgcgc tgctgctggc gccggcagtc gcgcgcgcgc atggactcaa
     4621 ggcaatcccc gtgcaatctg cggattctct gcaggaccca caggaggtcc aggacaaaga
     4681 acaggagaag cgcgaccgcg agcaggaggc acgcgacaga gaagaggaga aacgcgatcg
     4741 tgagcaggaa gcgcgcgaac gggcgcagga aaagatcgaa cgactgcaag aattgtacga
     4801 cgacgggcgt gaggacctgg atgaggaccg ctacgacggg gcagcgtcga aattcaagca
     4861 actggcggaa ctgaacggtc cgcaaacgga tgcagcgctc tactggaagg cgtacgcgga
     4921 gaaccgcttg ggcaggaggg acacggcact ggccaccatt gcggacttga agcggcgctt
     4981 tcctcagagt cgctggcaaa aggatgccgg cgcgctggag atcgaggtga agcagtcgac
     5041 cggccaacct gcaaagccag cggaccaaag cgacgaagaa ttgaaaatgc tagcgattca
     5101 agggctgatg aacagcgacc ccgaacgcgc tatgccgctg ctggaaacag ttttgaatgg
     5161 atcggcttca ccaaaagaaa agtcgaaggc actgttcgtt ctggcccagg gcgggtcaca
     5221 acaaggacgg gagattatcg gacgaattgc ccgcgggcag agcaatccgg agttgcagcg
     5281 gaaggcggtt gaatatctgg gactcttcgg cggtgctgaa gctcggaaga cgctggccga
     5341 cgtttacgca tcaagcggcg atgcttcggt gaagcacgcc atacttcgca gttacatgat
     5401 cggcggcgat cacgagcgcc tcttcgccgc ggcgaagagc gagaaggatg agtcactgcg
     5461 gcgcgaggcg atccggcagt tgggactggt tcgtgggacg agcgagctgg agcagttgta
     5521 ccagatggaa acttcgccgg acgtccggcg agaaatcctg caggcgctct tccttgcggg
     5581 cgattcagga aggctcgtgc aagcagcgca aggtgaaaaa gatgcggaat tgcgccggac
     5641 ggcgattcga aaccttgggc tgatccattc ggacgattcg gggaaggcgc tgcaagagat
     5701 ctacagcaag gaaacgaacc gcgaagtcaa agcagaagta ctgaacgcgt atttcctgca
     5761 agggaacgca aaagccctgg tggccatcgc gcgaagcgag aaagatccgg aactcaagaa
     5821 aacggcggtg tccaaattga cgctgatgca ttccaaggaa ggcaccgatt acctgatgga
     5881 actgctgcaa aagtgagagg gggagcgatg cgagaagaac gtactgattc ggccctccgg
     5941 agagtattcg gactcgcgat gatcgttgcg gggattggcg gtgccgccgt gcaattggca
     6001 aagggcgggg ccccggcccg gttagagcaa accagcccgg ccgcggcgca ggcgtcggaa
     6061 gcgccgcgga ttgaaaatgc gaagctggaa acgcgggcag ccggcgcaag tctcgatgcg
     6121 accatgcgcg agatcgcagc aacggcggaa aaagcggagt gggtcggcta ccacgtcgat
     6181 caggtcgctg gggagcgtgg cgtctgctgc aacaactcat ggaatgatgg gaattgcggg
     6241 acctgccggt tggaaaagga aaacggcgaa acgagcggca cgacgcacac ggacggaaac
     6301 gtcaagctgg aagggacgcg gcaactcgtc gtgctgtatc gcttggaagc gaagcaggtc
     6361 gtaaaaattc gggtcgcgtc gcaggattgc acgctggatg cgggaggact ggcgttcgtt
     6421 tggctgacag gagtcaaagc gacggagagc gtggcactcc tcgcaacatt cgtgagggga
     6481 tcggagttcg aagatcacgg ggatcgaggg ctcggcaacg gagcgttgac ggcgatcgtg
     6541 ttgcatgcgg gtgcgtcggc cgaccgtgcg ctcgaatcct ttgtgaagcc ggagcagcgc
     6601 gaaggtttgc ggagacaggc ggcattctgg atgggggcag cgcgaggcaa ggctggattg
     6661 actgcactgc aacaaatggc gaagtcggat ccgagtccgg acgtgcgcgc tcacgtggcg
     6721 tttgcgctgt ccgtgagcca tgaagctggt gcgctcgatg agatgatccg catggcgcac
     6781 gacgacacga gctcgcatgt gcgcgggcag gcgcttttct ggctggcgca agaggccgga
     6841 aagaaagcgg tgggaaccat cagcggcgca atcgagaacg atcccgacac ggatgtaaag
     6901 aacaaagccg tttttgcgct aagccaattg ccgaggtagg aaggtgtgcc gaagttgatt
     6961 gaagttgcac agacgaaccg gaatccggag gtgcgcaagc aggcgatgtt ctggttgggg
     7021 caatcgaatg atccgcgcgc gctgcagttt tttgaaaaag tgctgacgca gtgaagcgct
     7081 agcgcaaaag gtcttcgagc tggattctgg catccgtctt ggcgtcccgg tacttgacga
     7141 tgacggcggc ttgcagagag agttcccatt ttgcgccaac gggatgcgag acggcacgcg
     7201 aaccaggaac cacgatggct tcttcaggaa tgatcagcgg ctcggtctcg gtggcagtat
     7261 agattttgtc gcggacgaga tcgtaaaccg gaatggagcg attgaggatc gtgccggtgc
     7321 caagaacggc gcggcgcttc acgaccgttc cttcataaac gccggaattt ccaccgacca
     7381 ggacttcgtc ttcgataatc accggcagcg cgccgacagg ttcgaggacg ccaccgatct
     7441 gcgatcccgc agaaatgtgg caattgcggc cgacctgcgc gcaactccca acgagcgcgt
     7501 gcgaatcgat catcgtgccc tctccaacgt aagcacccac gtttacgaac atcggcggca
     7561 tgcaaatcac gccgcgaccg atgtaactgc catcgcgaat gctggagccg cccggaacga
     7621 tacgcacgcc gtcggaggat gtgaacgcgt gaacgggata ggtggactta tcgaagaagg
     7681 gctgcttcgc ggcatcgatc gacatgtcgg caatggcgcc catgcggaag ccgaggagta
     7741 ttcccttttt cacccaggcg ttcgcgcgcc agccggattt cgtggaggca tcgggttcgg
     7801 cggcacggat ttcgccgcga ttcagcgcgg ccttgaaatc gcggaagagc cattgatgtt
     7861 cttcttcgta gtgggccgga acttcgtcaa acaaccgctc aacttgcgat ggcagcatga
     7921 accctctcgg taccgggtgc ggccgcaact cggtcaacta gtcccagcga ttcgagcacg
     7981 gcccggatgc gcgcctgatt ctccgccttg ggcggcacga gtggcaagcg ccataccggt
     8041 tcgagcaaac ccatttccgc catcgcggcc ttcacgggaa tgggatttga ttcgacaaaa
     8101 tttatttcca tgagcggatg atagcgccgg tgaatcgcgc gcgcgctagg aaagtcgcct
     8161 tgcagggcga gcctcgtcag ttgcgacatt tccgccggga tttcattgga aacgacggag
     8221 atgacgccac ggccgccgag cgaaatgaca gggagcgtga tagcgtcgtc gcccgagagg
     8281 acgatgaagt catccggcac ggcattgaga atcgcgacca tttgggagac gttaccggaa
     8341 gcttccttga tgccaacaat attctcgatt tcggcgaggc gctttacggt ggcgggctcg
     8401 atgttcacgc cggtgcggcc ttgcacgctg taaagaatga taggcagcga aacggcttcg
     8461 gcaatcgcgc gatagtgctg gaagaggcct tcctgagtcg gcttattgta ataaggggtt
     8521 acggagagga tgccatccgc gccgatagcg gcgagctcgc gcgcgagtgc gataacctcc
     8581 gcggtattgt agccgccggc accggcgagg acggagacct ttcccttagc gagttcgacg
     8641 gtgatctgga caacacggag atgctcttcg ggcgtgagtg tggggctctc gccggttgtt
     8701 ccacaaggaa cgagaaagtc gatgcctgca tcaatttgcc gcttgatcag agctcgcaat
     8761 gcaggttcgt caaacgaacc atcgcgtcga aacggagtga caagtgctgt gccggtacct
     8821 gtaaacatga tgctgttcct tccttttttt cctcagccca attcgccgag gatctcgtga
     8881 aattcgaaaa gccctttttt cccaacgacc cagcgtgccg cgcggagcgc tccgcgcgca
     8941 aaaccttcgc ggttgcgcgc ggtatggcgc agcgtgatag tgtccgcgga ggaatcgaat
     9001 ccgatttcat gcgtgccggg atgcgctccc gcgcgattgg agcctacgct cacgggacgg
     9061 ttgtagccgt ttgcccgcat ctcctccgcg agttttttca gcgtgcccga aggcgcgtct
     9121 ttcttcgcgg agtggtgaat ttcccagccc caggcttcgt agtccgcgtg tttcgcaaag
     9181 agtgccgccg tgtgcgcaac agcctgcatg aaaagattta cgccaacaga aaaattaggc
     9241 gcccagacca agccagttcc gctcttcgtg acggcttcgc gggcagaggg aagatcatcg
     9301 aaccagccgg ttgtgccgac tatggtgttg atgcccagaa cggcaaggcg ccggatattt
     9361 tgcggcgccg cttgcggcgt cgtgaactcg acagccacat cgacgccgcg cagcgtttcg
     9421 tgagagagcg cctgcgcgtg cacattgttg ctgccatcga attttgcacg cacgtcaaaa
     9481 ccgtattcgg gcgcgaactg ctcgatgagg tggcccattt ttccgtaacc cacaatggcc
     9541 aggccgaggc tcatgcgctg ctccggccgg tcgcgaggag ttgggtcgcc atgcgcgcgt
     9601 agatttccac cgcttcagac aattcctttt tggagatgcg ctcctcgccg gtgtgcgcga
     9661 cgtgaatgct tccgggacca atgaggaagg gttgccccca ggcgctttcg aaacttggaa
     9721 tgtccgttgt gaaggccacc acggttgtcg gcaatccatc catcttcgcc aattgcaggg
     9781 ctggcgtgta gaagacctcg cgcgcttccg cacggccagc cgccgccgcg gaaacagctt
     9841 cccggatcat tgccggatcg ccgaccgtgc gaaacatgat ttccgcttcg gcgtgatcgg
     9901 cgacgacgtt gggtgcgcgg ccgccatcga tggtgccgac attcagagtg ctgcgtccaa
     9961 gcaaggcgtc ttgcggcaat ggaatctggc gaatgtcatg gagcacatcg agcagcgcgt
    10021 gaatggcgga atgaccgagc tcgggatagg cagagtgcgc gagttttcct tgtgtgatga
    10081 tttcgtagcg cagcgcgcct ttcgaaccga gagcgagccg gttctcggtg ggctcaccat
    10141 tgatgaggta tcgcgaacca cggggagtcg cggcggcagc ttttgctccg gcgctgttgc
    10201 gttcttcgcc gacgacgaaa aggaggccga gattgcgcgt gcccgcggcg agcagttttt
    10261 ccgccgcagc gatcatcgcc gcgatgatgc ccttcgcgtc gcaggaaccg cggccccaga
    10321 tgaattcgcc gtcctcgtga gaagcgaaaa acggtggcac ggtgtccatg tgcgtggaga
    10381 gcgtgacgac gggctcaccc caagtagcga agatattgtc gcgattgggt tcggcggaga
    10441 tgcgctcgat gcggccgtcg tgctgcgcgg ctagggcgga cagatgcgcg aagaggtagt
    10501 cgcccacatt tttttcgtgg ttggtggtcg attcaatgtc gacgagcgcg cgcgtcagtt
    10561 cgaaaagatt cattcgaatc aggcatttct ggatgagcga gacgaaagga aggaaccacg
    10621 aagatacaaa tcgaggctgc tgcctctcga tagcgcttca tcctaatgaa cggatgacag
    10681 tgaacaggcc ttgcgcctat tccccaaccg ccgcggtttc agctaccgca gcgatttcgg
    10741 cggacaacaa ctagttgcct tcgacctcac cggtcgaaag gagagcccct tttgcgcggg
    10801 catccgaagc tgctcggaat tgcttgcccg gctactcatg gcctccgcac ctctaccaag
    10861 aagagataag tctaatggaa ttagcgtcgg gatggcaaca aaatattagg cgagctaaat
    10921 tcaaaagatg gatgaatctc tttcaccgcg acgcgcgggg gactgttgct ggaggagctt
    10981 gcgatgcgaa cggcaaagca cggtgaattg aaccttctcg ggcggtatga gaacatgtag
    11041 ttctaactcc atgactcaag aacaagaaaa gaccgcttgg gagttagtca caaatcctcc
    11101 gcctgggagc gaactggcca gggcgaagga attcggcgtt gatctgactt tgttcatttc
    11161 aactctgcgg cgcacgccga ccgaacgggc acgaagcctc tccgagggcg cccgcatctt
    11221 ccagatagca aagcatccat agctaagtga gaaatgaaac tcgaagaaac gatccgtatt
    11281 ctgtatgacg cgagcgttga gttcgtggtc atcggcggag cggcgatggg tcttcagggt
    11341 tccgctcatc tgaccagaga catcggctct tgttacgccc gcactcccaa aaacatggag
    11401 cggcttgcga aagcccttga accattccac ccggtcctgc gtggtgtccc gcctaacctg
    11461 cccttccgct ttgatgccaa aacgattgcg tatgggctta attttacgtt gagcacggat
    11521 ttgggtgacc ttgatttcct gggcgaagta tccggtctcg gctcctttca agaggttctg
    11581 gcggcgtcgg atgtgagaga tgttggcgga gtggactgcc gagttctttc tctcgaagga
    11641 ctaatcaagt ccaaaaccgc agccggccgc ccccgagata tgtacgtgct acctgaatta
    11701 cgggggctca acgaatttaa gaaaaaaact ggtttggaat gatcgggaaa gtcttcccat
    11761 cattgaggag catgcttcac gcagcgttgg atcgtcggcc tacagctcga gaggcttgag
    11821 gggaacgtgc tgggaggcga cgagaagttc gccggtgaag gcagactcgg cagggcggcg
    11881 gcggagagct tcttcggcgg agatccgcac gagatcggga ttattgtttt cctgcgagac
    11941 gtgggcgagg acgagatagc gcgcaatggc gtcgaaaccg tcggggtcgc aaagatattc
    12001 gctgacggcg tgattcgaaa gatggccggt gcggctaagg acgcgctgct tgacgaccca
    12061 gggatacggg ccgaccttga gcatgtcgag atcgtgattg gattcgagga ccagacaatc
    12121 ggcgccgcgg agatggactt tgacgagctc gggcatgtac ccgaggtcgg tgacgatggc
    12181 catctttgcg ccgttagaac gaaaagtgaa gccgatgggg tcggcggcgt cgtgaggaat
    12241 ggcgaaagcg tggacgtcaa tgtcgccgac attgaaatgc tggccggggt gaatcgtttc
    12301 gacgcggttg agtcgcttgc cgaaggtttc cggaagaatg cgctgcatgg catccatggt
    12361 gggttcggtg acgtagagcg gagctttcca gaggccgagc atctgcggga gaccgttaca
    12421 gtgatcggtg tgctcgtggg tgatcaggat accgtcgacg tgctcgacgc ttctttcgac
    12481 agcggcgagg cgggcaagcg tttcgcgctt gccgagaccg gcgtcaatga ggaggcgggt
    12541 ccgcccggtc tcgagcaatg tgatgttgcc cgagctgcca gacgccagaa tggatacccg
    12601 caagccgatc gttttctcct gtactgcaac gcaaacaaat tttcagaatg atggcagagc
    12661 gccaagcgcc cgtcaacaag gactccacga ttgcaacggt gatatactcc cgccgcaaac
    12721 catggaaaag tacgatctca ttgtggttgg ttccgggccg ggaggccagc gagctgcgat
    12781 tcaagcggcg aagttcggaa agcgacccgc gcttattgaa aagctggaag tgatcggcgg
    12841 aacagcgatc aacaccggga cgatcccgag caagaccatc cgcgaagcgg tcctgcatct
    12901 ttccgggtat caatatcaga gcatttacgg cgtcaactat cgcgtgaaag aacagatcac
    12961 gatggctgat ctctccttcc gggccctcaa cgtaatcaag accgagatcg atgtgatccg
    13021 cgcccaactg tcccgtaacg gcatcgaagt tgtgacggga accgcgagct tcgtcgatcc
    13081 tcaccacatg caagtgacga gttcgcgagg acaatccgag tttgaagcgg acaacatcgt
    13141 gattgccacc ggcaccaaac ccgccgtctc tcccctggtt cctttcaaca acacgactat
    13201 catcaacagc gatcaaattc tgcggatgcc ggtgattccc aagacgatga tcgttgtggg
    13261 cggcggagtg atcggggtgg aatatgtgtg catgttcgcc accctgggcg tgcgcgtgac
    13321 attgattgaa aaacgtccgc gattgctgga attcgccgat gccgagatgg tggaggcgct
    13381 ttgttaccat ctgcgggatc ggcgcgtcac cttacggctg aatgaggaag ttgagagcgt
    13441 tgaagaaaca ccggatcata cggtcgtggc gaatctgaaa agcaagaaga aagttaccgg
    13501 tgaggcgctg ctctatgcgg tcggccgcca aggcaacgtg gatgaactga atcttgccgc
    13561 gggcggcctg gaggctgacg cgcgaggacg catcccggtg gacgagaact accgaaccaa
    13621 gcagcctcat atcttcgccg tgggagacgt catcgggttt cccagcctcg gctcggtttc
    13681 gatggaacag ggacgcatcg ccgccgccag agcgtttggc atgccgacga attccaaccg
    13741 cgcgaattat ccctacggga tttacaccat tcccgaaatc tcgtttatcg gaaagacgga
    13801 ggagcagctc accgaggaag gggtcccgta cgaggtcgga atggctttct tccgtgaaat
    13861 tgcccgaggc cagattcgcg gggacaccac cggccgcctg aaactcattt tcgatccgtc
    13921 caccaaggaa ctcctgggtg tgcacatctt tggtgaaggg gcctcggagc tgctgcacat
    13981 cgggcaggct gttttcgcgc tgaaaggcac ggtggaatat tttgtggata cggttttcaa
    14041 ttatccgaca ctggcggagt gttataaggc ggccgcattc aacggtctga acaaactggt
    14101 cagggcgtag tcacgtacga gtgaggagtc gattgcccga atcttcgact acttaagatt
    14161 ggcaggagca cgatgcgcag gacagttggt gtactggtca tggaacatat cgccgttggc
    14221 ctggtggaag gcgacaaggt cgtcggcccg ctgaacattt ttccggaaaa accgcaaacg
    14281 ctggacccgc tacaatcaat gcccgcggag agcatcgtgg aatgcatccg gcaacaggtc
    14341 gagaaagttg cagacggaga aaagatcgca gcgatcggaa tcggcttccc gggaattatc
    14401 cgttctggtg tcgtggagga ttctccaaac ctgcagcagg taaaaggatt cgcactgcaa
    14461 gcggctttgt catctgccct gggcggcaag gctgcgggag tgccggtccg gttgttcaat
    14521 gacgcggacg tcatggccgc gggaattgcg gcgacccaag gaaaattgaa ccaacttacc
    14581 cgcgtgtgga cgctggggaa tggagtgggt ttcgggcgct atccttcgag cgaaggcatc
    14641 tgggagggtg ggcactccgt ggtcacgctg gatcccaagg aaaacttctg tggatgcggc
    14701 gggcgcgggc acttggaagg cattctcggg cgccggtcca tgcgcatgcg cttcttggac
    14761 ctggagcccg aagaagtgtt cgaaaatgct cgtgcgggtg acgcgcgctg cgcggaattt
    14821 gtgacgcttt gtca
//
LOCUS       DASYVO010000507         9905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_252879, whole genome shotgun sequence.
ACCESSION   DASYVO010000507 DASYVO010000000
VERSION     DASYVO010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9905
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252879"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VGR03_17555"
     CDS             complement(<1..364)
                     /locus_tag="VGR03_17555"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV1996144.1"
                     /translation="MDQESVELKAETRPDGAPPNACFGCGDANAAGMHLPFERDAATQ
                     RVRGSFRLDARYQGAPGMIHGGIIAVVLDEALGKVCRFADVRAVTAELTIEYLRPIQV
                     DQEIHVEAFQVERKERNLF"
     gene            complement(433..2019)
                     /locus_tag="VGR03_17560"
     CDS             complement(433..2019)
                     /locus_tag="VGR03_17560"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV1996145.1"
                     /translation="MLPLQLQGESHVPLYVQIRDQLRAMVHSGELRPGDRIPASRELA
                     RHLGVHRTTVANAYAELESEGLIQGHVGRGTFIRENGSTGAPIGKGHAFGIGSASPVL
                     APAPGATSNGHLRWDTLFADERGEEALSRLMPAVPAGAISFIVARPAAEYFPIDKLTA
                     CAQAALKHDGHRVLQLGSSDGFAPLKRALCDLLKREGIKAKEDQILVSAGCQQALDLL
                     SKAFLRPGDAVILENPTYPGALAVFSAARVRCLGVNVRTVSSDSAGAGIDLEALETTL
                     VQNRVKLIVVTPDFQSPTGTTMPVGARRRLLDLATRHQVPIVEDHIYARLHFRSEPLP
                     SLKSLDRNGIVIHVDSFSKIAFPGLRVGWIVAPETVIDRLRLVKQYTDLHTDQLAQAT
                     MAEFVQQGFLEKHVKAMRRVYASRLSVLLDSMERHMPEGVEWTRPEGGMCIWVTLPPG
                     FDASELSVHARERGVLFSPGRYFYVQVPRPNAFRLGFAGVDERKIARGVEILGEELRM
                     EMRSRQRGNGRRSETRMALV"
     gene            complement(2187..2747)
                     /locus_tag="VGR03_17565"
     CDS             complement(2187..2747)
                     /locus_tag="VGR03_17565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996146.1"
                     /translation="MRRADLVRMPQNRIMPRTSQIVFGERQHLLRVLDSIEKTTMPSS
                     RVAAEKRSIESLIHARTKELDGINPGWDLKIGVVLNADTRAEALEAMAQEAPPEDYYL
                     LRLISEHPKAASKTLTRLAKHPYPAIRENVARHPNTDSVTLAKLCQDRTQPLWYLVAF
                     NPNTPAALRAKLQARMRKLGEAQQTA"
     gene            3127..4296
                     /locus_tag="VGR03_17570"
     CDS             3127..4296
                     /locus_tag="VGR03_17570"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEV1996147.1"
                     /translation="MNFNGMERQLAISQGGLMGVRPSAPVSLALLEQAAKEKLAPQAY
                     DYVAGGAGGEQTMRANRQAFHRWKIVPRMLRDVSTRDLSVSLLGVTLPAPVLLGPIGV
                     QGILHADAELATARAAVALGIPTVLSTVSSFSIEKVADANGDGVRWFQLYWGKDPDLT
                     ASMLQRAERTGYRALVVTLDTRLLGWRERDLQHPYLPFLAGEGLANYFSDPVFRARLA
                     QPPEKDPIAAIRLWGELFSNTALTWESLAFLRQHTRLPIVLKGVLHRDDALRAVDCGV
                     DGLIVSNHGGRQVDGAIASLDALPGVLDAVRGRFPVLLDSGIRRGADAFKAIALGATA
                     VLLGRLYIWGLAVAGEEGVRDVVLNFLADLDLTLALSGYTCFRELTREALVREDS"
     gene            4298..4576
                     /locus_tag="VGR03_17575"
     CDS             4298..4576
                     /locus_tag="VGR03_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996148.1"
                     /translation="MPAFDDHKDELEHYEQMLGRHRGRLAVSLDLVTNALVLVGQHGV
                     YCHSAGTPQKPVMDIRMIADELAKAKELIQSVMEELRAEREGRSPAGK"
     gene            4673..4954
                     /locus_tag="VGR03_17580"
     CDS             4673..4954
                     /locus_tag="VGR03_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996149.1"
                     /translation="MDARKRFLCSFLLILVATAAPMVSAAQTLRVNEAATRIHPLGKG
                     LEVSLVLDNSGPLTSGHASLELILRDDRVRAAGAARAIRMIPSTNSPPP"
     gene            4951..6333
                     /locus_tag="VGR03_17585"
     CDS             4951..6333
                     /locus_tag="VGR03_17585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEV1996150.1"
                     /translation="MTTGSTAYPKWGLSWTYDRYGNRTDQNQTAGSPPMNHVTVDATT
                     NRIITTGYTYDVGGNMTNDGSNTLTYDAENRGATAAGATYTYDGHSLRVKKVSGSTTT
                     VYIFSGSQVVAEYVNGAAPTAPTREYIYSGGALLARIDSSGTKYYHQDHLSNRLVTDS
                     SGNTSAQLGHFPFGESWYNTSSDKLLFTTYERDAESGNDYAMARYHVNRLARFSSPDP
                     LAGSIASPQSLNRYAYVRNDPINSIDPLGLFTDNPPLPPDPIGDGPTFGPDFGFLGGG
                     GIKGDSPRRIGLDDKNKKQFEKAKTKALNNVENPDCQAFLKSRSIDPSAVADAIANEV
                     PWDGTKSSISQYDAGVLDPQDDVWKTQRGIDSAKATSVNRYFNASGTGSTVKAEAQTP
                     GHDVYFQPGTYLLSFKLSSGGITPGIIIHEGLHNLTGLGDTALANKLGLPAGAGSAQI
                     NPLLAANHCI"
     gene            complement(6886..7047)
                     /locus_tag="VGR03_17590"
     CDS             complement(6886..7047)
                     /locus_tag="VGR03_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996151.1"
                     /translation="MENGSNYAVRLACFPKAQNHIRFCAECGEQVSVVNKAEDSRPGR
                     AVAISPVNT"
     gene            7400..8293
                     /locus_tag="VGR03_17595"
     CDS             7400..8293
                     /locus_tag="VGR03_17595"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEV1996152.1"
                     /translation="MNLVEMIQQFNSEAKCRAYIELLRWPDGVACPHCQSRKIYRLEN
                     RPLLLCASCEHQFSVTVDTVFHDTHLPLEKWFLATFLLCESKKGISACQIQRTLGVSY
                     KTAWYLCHRIRAAMLEIAPEKLSGTVEIDETYVGGKARRWRPRSDKAVVIGIRKRNGE
                     LRLIRAKDATANTVQEIIDAHVGQDVEVIMTDESAIYPWALKKLPKEKHKTINHTKEY
                     AHGDIHTNTIESAFSLLKRGIVGTWHKVSAKHLPAYLDEMCFRFNNRKNSFLFRDTIL
                     KLIASPNLEYKDLTAKVQDAA"
     gene            complement(8300..9025)
                     /locus_tag="VGR03_17600"
     CDS             complement(8300..9025)
                     /locus_tag="VGR03_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996153.1"
                     /translation="MWDAFWKWLGIFGLVVGLMSGILSLFRELLGVYRPDQAHAASLF
                     WRCVWIAFIISSVSAWASQYHRANEAERKLEETKQPSFKVTIGQSISVYRADQKATFW
                     LPVITIVNGGAQSAILGWQIHYKSPTLDQDVPPVRILEKTFELEIPNQPVFVMNPLEA
                     VNLKKGPIVRGDSREGRLPVFIPGDHTKEISSGEALISVTVHDYLGNSYTGEFRGEPN
                     EGLRFLPEEGTKESLKRQKKLRD"
     gene            9353..9748
                     /locus_tag="VGR03_17605"
     CDS             9353..9748
                     /locus_tag="VGR03_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996154.1"
                     /translation="MMLIAVALLVYVAAAVAQDPVKVDPKHYKVTMENDNVRVLRISV
                     PPHESAPMHSHPASVLIWITDSHIKLVHPDGKTEEAFRKAGQTNWSETEVHAGENIGD
                     TVSECVQVEFKKPFAKIAPTKPTAKAKTQ"
BASE COUNT         2082 a         2980 c         2865 g         1978 t
ORIGIN      
        1 gaaacaggtt gcgctccttg cgctcgacct ggaacgcctc cacgtgaatt tcctgatcca
       61 cctggatcgg ccgcaggtac tcgatggtca gctccgcggt cacggcgcga acatcggcga
      121 agcggcagac ttttccgagc gcctcatcga gaaccacggc gattatacct ccgtgaatca
      181 ttccgggcgc gccctggtag cgggcgtcga gacggaagct gccacgcacg cgctgtgtgg
      241 ccgcgtcgcg ctcgaacggc agatgcatgc cggcagcgtt ggcatcgccg caaccaaaac
      301 aggcgttcgg cggcgcgcca tcggggcgcg tttctgcttt caattccacg gattcttgat
      361 ccaccgcgtc ctctcttccc caccgttgtt cggatggcgc tgttcgccgc gacccctacg
      421 acccgcgcta ccctacacca gcgccatgcg cgtctcgctc ctgcgcccgt tgccgcgctg
      481 gcggctgcgc atttccatgc gcaactcttc gccgaggatt tcgacgccgc gggcgatttt
      541 gcgctcgtcg accccggcga aacccaggcg gaaggcattg ggccgcggaa cttgcacgta
      601 aaaatagcgg ccggggctga acagcacgcc gcgctcgcgc gcgtgaacgg agagctcgct
      661 ggcgtcgaag ccgggcggca gtgtaaccca gatgcacatc ccgccctcgg ggcgcgtcca
      721 ttccacgccc tcgggcatgt gccgttccat gctatcgagc agaacgctca aacggctggc
      781 ataaacgcgc cgcatggcct tcacgtgttt ttcgagaaag ccctgttgca cgaactcggc
      841 catggtggcc tgggccaact ggtcggtgtg caggtcggtg tactgcttca ccaaacgcag
      901 ccggtcgatc accgtttccg gggcgacgat ccatccgacg cgcagaccgg gaaacgcgat
      961 cttcgaaaag ctgtcgacgt gaatcacgat gccgttgcgg tcgagagatt tgagcgaggg
     1021 cagcggctcg ctgcgaaaat gcagccgcgc gtagatgtgg tcttcgacga tgggcacctg
     1081 gtgtcgcgtg gcaagatcca gcagccgccg gcgcgcgccg accggcatgg tagtgccggt
     1141 gggactctga aaatccggcg tgacgacgat gagcttgacg cgattctgca ccagcgtggt
     1201 ctccagcgcc tcgaggtcga tgcccgcccc ggccgagtcg ctggaaacgg tgcgaacatt
     1261 tactcccagg cagcgcacgc gcgccgcgga gaacaccgcc agcgcgccgg gatacgtcgg
     1321 attttcgagg atcaccgcgt cccccggccg cagaaacgcc ttggacagca gatcgagcgc
     1381 ctgctggcaa ccggcgctga ccaaaatctg atcttccttc gccttgatcc cttcgcgttt
     1441 tagaagatcg cagagcgcgc gcttgagggg agcgaatcca tcggatgagc cgagctgcag
     1501 cacgcggtgg ccgtcatgct ttaatgccgc ctgagcgcag gcggtcagct tgtcgatggg
     1561 aaaatattcg gccgcaggcc gcgccacgat gaacgagatg gccccggcag gaacagccgg
     1621 catgaggcgc gacagcgctt cctctccgcg ctcgtcagcg aaaagcgtat cccaacgcag
     1681 atggccgttc gacgtcgcgc cgggcgcagg cgccagaacg ggggaggcgg agccgatgcc
     1741 gaatgcatgg ccctttccaa ttggagcgcc ggtggaacca ttttcacgga tgaacgttcc
     1801 gcggccgacg tggccctgaa tcaggccttc ggactcgagc tcggcatagg cgttggcaac
     1861 cgtggtccgg tgaacgccga gatgccgggc caactcgcgg ctggccggaa tgcgatcgcc
     1921 gggccgcaac tcgccgctgt gaaccatggc gcgcaactga tcgcggatct ggacgtaaag
     1981 tgggacatgg ctttcgccct ggagttgtag tggcagcatt ggctctctcc ggttcctact
     2041 ttacaccgga ttggacggtc gaaaagagcc aatatgtctc cctctcaagc gggcacggta
     2101 tgcggccatt ctggacagat cgaaacgatg cggtgggaga gaagagaccc cggagagcag
     2161 gcgccctgcc cgatccgaga ttcgagctag gcggtctgct gcgcctcgcc gagctttctc
     2221 atgcgcgcct gcagtttggc ccgcagggcc gcaggggtgt tgggattgaa ggccacgagg
     2281 taccacagcg gctgcgtgcg atcctgacag agcttggcga gcgtgacgga atccgtgttg
     2341 ggatgccgcg ccacattttc tcgaatggcc gggtagggat gcttggcgag acgcgtgagg
     2401 gtcttcgacg cagctttggg gtgctcgctg atgaggcgca acagatagta atcctcaggc
     2461 ggcgcttcct gggccatggc ttcaagcgct tccgcgcgcg tatcggcatt gagcaccacg
     2521 ccgattttca aatcccatcc gggattgatg ccgtcgagct ctttggtgcg cgcatggatc
     2581 agggattcga tggagcgctt ttcggcggcc acccggctgc tgggcatggt ggtcttctcg
     2641 atagaatcca ggacgcgcag cagatgctgg cgctcgccaa agacgatctg cgaagtcctg
     2701 ggcataatac ggttctgcgg catccgaacc agatcagcac gcctcattat tattcacctc
     2761 aaaacctatt cacctcaaag attacgtcct gcattccgca gggcgtggcc ggaagtgccg
     2821 cggagggccc cgccgtcaaa ctcgagacgg gagctgatgc ctgagagact cccccggcct
     2881 ggatgtgaag cccatgccgc cgcaacctcc ggtccgcccc cgcgaaccat gcgaacaccg
     2941 ccactcactc caggatgaat tatccaaacc gctgcacggc gagtaaaggc gggtggccgc
     3001 ggatggttcc atgcctgcaa catgcactta ccggccgccg cgggcgtcgc ttgcgccaat
     3061 cggtcgaaca cgctagcttg tgtggcgcgg cgaggcaagg gatcagattg tatggggtaa
     3121 gatgggatga atttcaacgg catggagcgc cagctggcga tttcgcaggg cgggttgatg
     3181 ggcgtgaggc catcggcgcc tgtgtcgctg gcgctgctgg agcaggcggc gaaagaaaaa
     3241 ctggcgccac aggcctacga ctacgtggcc ggcggcgccg gcggcgagca aaccatgcgc
     3301 gcaaatcgcc aggccttcca ccgctggaag attgtgccgc gaatgctgcg cgacgtatcc
     3361 acgcgagacc tctccgtatc tcttctgggc gtgacgctgc ccgcgcccgt gctgctgggg
     3421 ccgatcggcg tgcagggcat ccttcatgcg gatgcggagc tggctacggc gcgcgcggcg
     3481 gtagcgctcg gcattcccac ggtgctgagc acggtttcat ctttttccat cgagaaagta
     3541 gccgacgcca acggcgacgg cgtgcgctgg tttcaacttt actggggcaa ggaccccgac
     3601 ctcaccgcca gcatgctgca gcgcgccgag cgcacgggct accgcgcgct ggtggtgacg
     3661 ctggacacgc ggcttctcgg ctggcgcgag cgcgatctcc agcatcccta tcttcccttc
     3721 ctggccggcg agggtttggc aaattatttc agcgaccccg tttttcgcgc gcgcctcgcg
     3781 cagccaccgg aaaaggaccc catcgcggcg attcgtctgt ggggcgagct gttttccaac
     3841 actgcgctta cctgggagag tctcgcgttt ctgcgccagc acacgcggct gcccatcgtg
     3901 ctgaagggcg tgctgcatcg cgacgacgcg ctgcgcgcgg tggactgcgg cgtggatgga
     3961 ctgatcgttt cgaatcacgg cggccggcaa gtcgacggcg ccatcgcgtc gcttgatgcc
     4021 ctgcccggtg tgctcgacgc agttcgcggc cgcttcccgg tgctgctcga cagcggcata
     4081 cgccggggcg ccgatgcgtt caaggccatt gcgctcggcg cgacggcggt gctgctcggc
     4141 aggctgtaca tctggggact cgccgtggcc ggcgaggagg gcgtgcgcga cgtggtgctg
     4201 aattttcttg ccgatctgga tttgacgctg gcgctgagcg ggtacacctg cttccgcgaa
     4261 ctgactcgcg aggcgctggt gcgcgaggat tcttaaaatg ccggcattcg acgaccacaa
     4321 agacgaactg gagcactacg agcagatgct gggccgccat cgcggacggc tcgccgtatc
     4381 tctcgatctg gtgaccaacg cgctggtcct ggtcggccag cacggagtct attgccattc
     4441 cgcgggaact cctcagaagc cggtgatgga catccgcatg atcgcggacg agctcgccaa
     4501 ggccaaagag ctgatccaga gcgtgatgga agagctgcgc gccgaacgcg aaggccggtc
     4561 cccggccggc aaatgaatgt caattctgcc gcgacaggca acctcagcac cgccagcgac
     4621 aacaaaccaa gtaagagacg gcgttctgca atccgtgacc ggaggtgggg ttatggacgc
     4681 gcgtaaacgc tttttgtgtt cctttctcct gattctcgtc gcgactgctg cgccaatggt
     4741 ctctgccgcg cagaccttac gcgtgaatga agcggcgacg cgcatccatc cgctcggcaa
     4801 aggtctcgaa gtgtctctcg ttctggacaa ctctggcccg ctgacgtctg ggcacgcaag
     4861 cctggagttg atcctgcggg acgaccgggt tcgcgccgcg ggcgcagcgc gagctatacg
     4921 tatgattccc tctaccaact ctccaccgcc gtgaccactg gatccacagc ctatcccaaa
     4981 tggggattgt cttggactta cgaccgctac ggcaatcgca cggaccagaa tcagacggcc
     5041 ggcagcccac cgatgaatca cgtcaccgtc gacgccacca cgaaccgcat catcacgacg
     5101 gggtatacct atgacgtggg cgggaacatg accaacgacg gctcgaacac gttgacgtac
     5161 gacgcggaga accgcggggc gactgcggcc ggcgccacct atacttatga cggccacagc
     5221 ctgcgggtga aaaaagtctc cggcagcacg accacggtgt acatcttctc cggctcccag
     5281 gtcgtcgccg agtacgtcaa cggcgccgcg cccaccgccc cgacccgcga gtacatttac
     5341 tccggtggcg cgcttctcgc ccggatcgac tcctcgggca cgaagtatta tcaccaggac
     5401 cacctctcga accgcctggt caccgattcc agcggcaaca cctccgccca gctcggccac
     5461 tttcctttcg gcgaatcctg gtacaacacg tcgagcgaca agctgctatt taccacctac
     5521 gaacgcgacg ccgaatccgg caacgattac gccatggccc gctatcatgt caaccgcctc
     5581 gcgcggttct cctcgcccga cccccttgct ggctcaatcg caagtccaca atcgctgaat
     5641 cgctacgcct atgtacggaa cgacccgatc aattcaatcg acccgctagg actttttacc
     5701 gataaccctc ctctcccacc tgatccgatt ggagatggcc ctacattcgg tcctgatttt
     5761 ggtttcttgg ggggcggcgg aattaaaggc gattctccgc gccgaatcgg tctggacgac
     5821 aagaacaaga agcaatttga aaaggcgaaa actaaggcgt tgaacaatgt cgagaacccc
     5881 gactgccaag ctttcttgaa atcgcgtagc atcgacccgt cggctgtagc agatgccatc
     5941 gcgaatgagg tgccttggga tggcacaaaa tccagcattt cgcaatacga tgctggtgta
     6001 ctcgacccgc aagacgatgt ttggaagaca cagagaggga tagacagcgc aaaagccaca
     6061 tcggtcaatc gctactttaa cgcatcaggg accggaagca cagtgaaggc tgaggcccag
     6121 actcccgggc atgacgtcta ttttcaaccg ggaacgtatt tgctctcgtt taaattgagc
     6181 tctggaggaa ttacgcccgg tatcatcatt cacgaggggt tacacaactt gacgggcttg
     6241 ggcgacacag cactggcaaa caaattggga ttacccgccg gcgccggaag cgcacagatc
     6301 aatccattgc tggcagcgaa tcattgcatc tgagaaccca gggtcttggg agcttcgatg
     6361 atcttaatgg tcttacaagc taagcgcgta ttcctcggcg cgaccattgg cacaatcctg
     6421 ctcatgttac cctcccagtc aagccaggag attcggtcga aggaaacatg ccaaagcgac
     6481 caaaaacatg ctgccccccg atatcgtgtt gccaagaaat accggacgga tctcaaacct
     6541 gggctagtta tgttcgtgag cgtggctccc tctgagatcg accgcgataa acttattgcg
     6601 ctcgcatgcg aactggggcg aaagtacttg aacgaggacc gcctctctgt ttggatcttg
     6661 gacagctatc gtgcggcaag acgattccat ccacaaggcg agggcaatga caggcagaca
     6721 aacctttctc gtcgagcgct attcggtttc tcgcgggaaa atggcgatgg ggagcagtcc
     6781 ttggaatggt ggcctgaccc catcgaccga aatcgaaggg ttcacattga cctaggggat
     6841 gcccctcgaa aagaggcgga acaccgtcga tgagggccgg atggctcagg tatttacggg
     6901 cgagatcgcc acggcgcggc ccgggcggct gtcctcggcc ttgttgacca cgctgacctg
     6961 ctccccgcat tccgcgcaga agcgtatatg attttgggcc ttcgggaagc aggccaaccg
     7021 caccgcgtag ttgcttccat tctccataac tttcctcttg caatatggta accaacgagg
     7081 tatcctatcc ggcgtgagcg ccatgccagc cactcgacac gatccactga ccactcgtct
     7141 cggctctcca actcctttat ttctcacact tacgaactgc ctcctaagtt catcaaatcg
     7201 ccgggcttta agcgtgatta tatacttgac ttcacttcgc atttctgtta gcctttcttc
     7261 gtgcttgata cccgctcaat cttagccaag tgccgcttga taatcgtgtg gcctttcttc
     7321 gtaaataggt gctgcatcgc ctcgtctgtt gtcatgtcct tcgggtgttt ctttattttc
     7381 ttcggcatgg gagcgtccta tgaatctcgt tgagatgatt cagcaattca acagtgaagc
     7441 caagtgtcgc gcctacatag aactgttgcg ctggcccgat ggcgttgcgt gcccgcattg
     7501 ccaatcgaga aagatatacc gccttgagaa tcggcccctc ttgctgtgcg cctcatgcga
     7561 acaccaattc agcgttaccg tggataccgt cttccatgat acacacctgc cgctggaaaa
     7621 atggttcctc gcaacattcc tgttgtgtga atcgaagaaa ggaatatcgg cctgccagat
     7681 tcaacgcacg ctcggcgtga gctataagac ggcgtggtat ctgtgccaca gaatccgcgc
     7741 tgccatgctt gagatagcac ccgagaagct cagcggcacg gttgagattg atgaaaccta
     7801 cgtcggcggc aaggcccgga gatggcgtcc acgttctgac aaggccgtgg ttatcggcat
     7861 ccgcaagcgc aatggagagc ttcgcctgat ccgcgccaag gatgccacag cgaacacggt
     7921 gcaggagatt attgacgcgc atgtcgggca ggatgtggaa gtgattatga ccgatgaatc
     7981 cgccatctat ccgtgggcct tgaaaaagct cccgaaggaa aaacacaaga ccatcaatca
     8041 caccaaggaa tacgcgcatg gcgatattca caccaacacc attgaatccg ctttctctct
     8101 cctgaaacgc ggcatcgttg ggacgtggca taaagtcagc gcgaagcatc tcccggccta
     8161 tcttgacgaa atgtgtttcc gattcaacaa tcgcaagaac tctttcctct tccgcgacac
     8221 catcttgaag ctaattgctt ctccaaacct cgaatacaaa gacctgactg ccaaagtaca
     8281 agatgcagcc taagtgggtt tagtcccgca atttcttctg tcttttaagc gattctttgg
     8341 ttccttcttc tggcagaaat cgtagcccct cattcggttc ccctctgaat tccccagtgt
     8401 atgaatttcc taagtaatcg tggacagtta cggaaattag agcctccccg cttgatattt
     8461 ctttagtgtg atcccctgga atgaagactg gcaaccgacc ttccctagaa tccccgcgca
     8521 cgattggacc ttttttcaag ttaacagcct ctagcgggtt cattacgaaa acaggctgat
     8581 tcggaatctc cagctcaaac gtcttttcaa ggatacgaac gggaggtaca tcttgatcta
     8641 gcgtcggaga tttgtagtgt atttgccatc cgagtattgc actctgcgcc ccgccattta
     8701 caattgtgat gacagggagc cagaaagttg ctttttggtc ggcacggtaa acacttatgc
     8761 tctgcccgat tgtaacctta aaactgggct gtttagtttc ctccagcttt ctttctgctt
     8821 catttgctct atgatactgc gatgcccatg cagaaacaga cgagattatg aaagctatcc
     8881 aaacacatct ccaaaataat gaagcagcat gagcttggtc tggacggtaa actcccagca
     8941 gttcgcggaa taaagaaaga atcccgctca tgagacccac cacaagaccg aaaattccaa
     9001 gccatttcca gaacgcatcc cacatggcag aagcacactc cttcgcgact taaaacggga
     9061 agggtagctc tcggcggcgg gtgaagtcaa atacataatc ccgctttaag tcacgtatcg
     9121 gcactacata cgagccccca taccctaacc cctttattgg cactacatac aagaataagg
     9181 ggggaggggg gaggtacctc aaacgttaac agctcactcc aatcgaggat ccatcgcaag
     9241 accacccacg cggaccggcg agcctgctag tatgggggcg caatctgcgc ctccccgagg
     9301 gagagcgaaa ggtgaatcgc tgatgaggaa tacaggaaga actttctgcc gcttgatgct
     9361 tattgccgtc gctctgctgg tgtatgtggc tgcggcggta gcgcaggacc ccgtcaaggt
     9421 tgaccccaag cattacaagg tcaccatgga gaacgacaat gtgcgcgtgc tgcgcatctc
     9481 cgtgccgccg catgagagcg cgccgatgca ctcgcacccc gccagcgttc tcatctggat
     9541 taccgactcg cacatcaagc tggtccatcc cgacggcaaa acggaggaag cctttcgcaa
     9601 ggcggggcag acgaattggt ccgagacgga agtgcacgcc ggagaaaaca tcggcgacac
     9661 ggtgagcgag tgcgttcagg tggagttcaa gaaacccttc gcgaagattg cgccaaccaa
     9721 gcccaccgca aaagcgaaga cgcagtaatc cccgctgacg cgttcagcgc atgcgaaaat
     9781 ccggctgaag gaagggatta ctctcgcgtt cgcggccaat cgttgtggcg gggccgtggc
     9841 cgggaaaaac acgcgtgtcg tcgggaagcg ccaaaagttt ttcccgaagg gagcgaagaa
     9901 tatcg
//
LOCUS       DASYVO010000508         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_210439, whole genome shotgun sequence.
ACCESSION   DASYVO010000508 DASYVO010000000
VERSION     DASYVO010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210439"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VGR03_17610"
     CDS             complement(<1..562)
                     /locus_tag="VGR03_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996155.1"
                     /translation="MLNRLIQKLLISFPLLLCPFFSAQSASPPQSARWTEKAANDWYA
                     KQPWLVGSNYIPANAINELEMWQADSFDPQRIDLELGWAESLGLNTMRVFLHDLPWAQ
                     DPEGFRKRIDTFLQIAAKHHIKPLLVLFDSCWDPDPRLGKQRDPRPGVHNSGWVQSPG
                     AKALQDQAQVPRLEKYVKGVLGAFAND"
     gene            complement(712..1761)
                     /locus_tag="VGR03_17615"
     CDS             complement(712..1761)
                     /locus_tag="VGR03_17615"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1996156.1"
                     /translation="MSFDELNRAPHRRFNPLLREWVLVSPHRTQRPWLGKVEKLQKPS
                     AVTFDPNCYLCPGNKRAGGAQTPPYTETYVFDNDYPAMLPEVAPLQFDTAGLLVGKTE
                     QGICRVMCFSPRHDLTVPRMSLQESRGVVNGWVEQYSALGQISWIHHVQIFENRGEMM
                     GASNPHPHCQIWANASLPNLPTRELASFREYRVEKHSCLLCDYLKLELQLKVRVVCQN
                     DAFAVVVPFWAVWPFESLVLSKRHLASIDQLTGEERELLGDIMRRITIRYDNLFETAF
                     PYSMGFHQKPTDGGAYDEWHLHAHYFPPLLRSATIQKFMVGYELLGSPQRDVTPESTA
                     NRLLEAGDIHYLDRS"
     gene            complement(1826..>2443)
                     /locus_tag="VGR03_17620"
     CDS             complement(1826..>2443)
                     /locus_tag="VGR03_17620"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996157.1"
                     /translation="CRSLKHDFVKLPPDLQLVICNTMVRHELASGEYNARRAECEEGV
                     RILRIVLPEIRALRDVTLAQLEEHRRILPSLVTARCRHVITENVRVKSAVEAFHRGDS
                     KALGPLMQDSHRSLRDDYEVSCRELDLMVEIASAQPGLIGARMTGGGFGGCTINLVES
                     AAVADFRRNVAAAYSAKTGLTPEIYVSPASEGAQQMALEENKPAK"
BASE COUNT          518 a          675 c          734 g          516 t
ORIGIN      
        1 tgtcgttcgc gaacgcgccc agcacaccct tcacatattt ttcaaggcgc ggaacttgcg
       61 cctggtcctg gagcgccttc gctccggggc tttgtaccca gccggaattg tgcacgcccg
      121 ggcgtgggtc acgctgcttg ccgagtcgtg gatccgggtc ccagcaggaa tcgaaaagaa
      181 cgaggagcgg tttgatgtgg tgcttcgccg cgatctggag aaaggtatcg atccgcttgc
      241 gaaaaccttc ggggtcctgt gcccagggca ggtcatggag aaagacgcgc atggtgttga
      301 ggccgaggga ctccgcccag ccaagctcga ggtcgatgcg ctggggatcg aaggaatccg
      361 cctgccacat ttcgagttcg ttgattgcat tggcgggaat gtagttgctg cctacgagcc
      421 agggctgctt ggcataccaa tcgttggcgg ccttctcggt ccagcgcgca gactgtggcg
      481 gcgatgctga ctgcgcagaa aagaatgggc agagcaagag cgggaaacta atcaacagtt
      541 tttgaatcaa tcgatttagc acttttgtac tcgattcctg aaacatctca gttgcaattc
      601 aatggatgag atcctttccg gcaaagccgg aaccttcggt cgcttcgctc tggatgacat
      661 tggctgcgag tttggcctaa agaatacgcg tttcatagtg agagatccag actaggaacg
      721 gtcaagatag tgaatgtcgc ccgcctcgag gagccgattc gcagtgctct ccggcgtgac
      781 gtcgcgctgg ggagagccga gaagttcgta gccaaccatg aatttttgga ttgtggcgga
      841 gcgcagtaga ggcggaaagt agtgcgcgtg caggtgccac tcgtcgtagg caccgccgtc
      901 ggtgggtttc tggtggaatc ccatggaata ggggaaagca gtctcaaaca aattgtcgta
      961 gcgaatggtg atgcggcgca tgatgtcgcc caggagttcc cgctcttcgc cggtgagttg
     1021 atcgatcgaa gcgaggtggc gcttgcttag cacgagcgat tcgaatggcc aaacggccca
     1081 gaagggcacg accacggcaa aagcgtcgtt ctggcagacg acgcgcactt tcaactgcag
     1141 ctctaatttc aggtaatcgc acaacaggca ggaatgtttt tcgacgcgat actcccgaaa
     1201 ggacgccagc tcgcgagtgg gaaggttcgg cagcgaagca ttcgcccaaa tctggcagtg
     1261 cgggtgggga ttgctcgcac ccatcatctc gccgcgattt tcgaaaatct gcacatgatg
     1321 aatccaggaa atttgcccga gtgcggaata ctgttcgacc cagccattca cgactccgcg
     1381 cgattcctgg aggctcattc gcggaacggt gagatcgtgc cggggagaaa aacacatcac
     1441 gcggcagatt ccctgctctg tttttcccac cagcaagcca gccgtatcaa attggagagg
     1501 cgcaacctcc ggcaacatcg ccgggtaatc gttgtcaaaa acataggttt ccgtgtacgg
     1561 cggcgtctgt gctccccccg cgcgcttgtt gcccgggcac aaataacagt tgggatcgaa
     1621 agtgaccgcg gaaggtttct gaagcttctc tacttttccc aaccacgggc gctgggtgcg
     1681 gtggggtgag acaagaaccc actcccgcag gagcggattg aaacgccggt gtggtgctcg
     1741 atttagctcg tcgaaactca taggcaaagt cgaaccttga cggatcctgg caactcgctg
     1801 cggttccctt ctccgctagt tcctgtcact tggcgggctt gttctcttcg agggccattt
     1861 gctgcgcgcc ttcggacgcc ggagaaacgt aaatctcagg agtcagacct gttttggcgg
     1921 aatatgccgc ggccacgttg cgcctgaaat cggccacggc cgcggactct accaaattga
     1981 tggtgcagcc gccaaatcct ccgccggtca tccgcgcgcc aatcaggcca ggttgcgcag
     2041 aggcaatttc caccatcaga tcgagttccc tgcagctgac ctcatagtcg tcccggaggc
     2101 tgcggtgcga atcctgcatc aacggcccca gggctttgct gtctccccgg tgaaatgcct
     2161 cgaccgcgct tttcacgcgc acgttttccg taatgacatg gcggcaccgg gcggtgacca
     2221 gtgacggcag gatgcgacgg tgctcctcca actgagcgag tgtgacgtca cgaagggcgc
     2281 gaatttccgg caaaactatc cgcaagatgc gaacaccctc ttcgcactcc gcgcggcgtg
     2341 cattgtactc gccggaagct agctcgtgcc ggaccatggt attgcagatt accaactgga
     2401 ggtccggcgg aagtttgacg aaatcgtgct ttagcgaacg aca
//
LOCUS       DASYVO010000509         5432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_83423, whole genome shotgun sequence.
ACCESSION   DASYVO010000509 DASYVO010000000
VERSION     DASYVO010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83423"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VGR03_17625"
     CDS             <1..279
                     /locus_tag="VGR03_17625"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1996158.1"
                     /translation="ASDESVAAADVRLILPTSDSPSGSTSASSAGSSTGTLAERTEAF
                     EREVMLAEIRRHNFHMTNVARALGLERSHLYKKCQQLGIDLQSLRKPE"
     gene            391..921
                     /locus_tag="VGR03_17630"
     CDS             391..921
                     /locus_tag="VGR03_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1996159.1"
                     /translation="MKLGDKLRSLRAVEGSLRGLGRSMTQQELATAMKSEIGRGLSQA
                     YLSQIENGARPHLTHTTRELLARFFRVYPGFLVDDPEGYTPGLQSELRAIDAKIDSWL
                     FAGAEQFTADPVLQRALQTIAEHEDSRRAILLLAEILRTPDLAERLGEVLHPGNHQNG
                     GQNGNAHPPPSAALRN"
     gene            1013..1603
                     /locus_tag="VGR03_17635"
     CDS             1013..1603
                     /locus_tag="VGR03_17635"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEV1996160.1"
                     /translation="MAWSDFYLFCFIVGFSLSVLSFLAGAVHIHLPFRLHLPFQGGHH
                     AGGLHSSGHAHAGGHSSWFNTSTLLAFLAWFGGTGYILTNYSNLVAIASLGLATLAGL
                     VSGSIVFKFMVKLMHSSEAPLNEADYRVEGSVCTVTLPIRANGTGEVVFLLGGVRRSA
                     GARTDDGKPIEKGAEVVIERHENGIAYVKRWDEFTS"
     gene            1704..3146
                     /locus_tag="VGR03_17640"
     CDS             1704..3146
                     /locus_tag="VGR03_17640"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1996161.1"
                     /translation="MWGFPLVPVVAGLAVLGVILVMATVARMYRKVGPHEALIIYGMG
                     GTRIVKGSGAVIFPMIQTCKELSLELMSFDVAPQQSLYTQQGVAVTVEAVAQIKVKSD
                     PVSIRTAAEQFLSKRPEQREGLIRLVMEGHLRGIIGQLTVEQIVKEPEMVADRMRSTN
                     ASDMNKMGLEVVSFTLKEVRDKNEYITNMGRPDVARIKRDAEVAAAEADRDIAIKRAE
                     ATRASAVAKAQADQERVAAETASLAKQAEAERDLDLKRASYLEMTKKAQAMADKAYEI
                     ETNIMQQQVITESVRVQQVEREAQVKVQEAEIERRERELIATVLKPAEVERQRIETLA
                     SAERQRSITEAEGRASAIRAQGEAEAEIIFKKGEAEAKAMNVKAAAFQEYNQAAVLDK
                     LISGLPEIVRALSEPLSKVDKVTIVSTGNGDAAGAYKLTGDITRIAAQVPALFEALSG
                     MQMSELLSKVRLIGDKAPKPEPPQAPIKKA"
     gene            3214..3399
                     /locus_tag="VGR03_17645"
     CDS             3214..3399
                     /locus_tag="VGR03_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996162.1"
                     /translation="MSMKEKLLFVWLLVVVAFSFVFHHFWASTICALGRVVALNLVSL
                     PGLAKFAAGICGVHLLP"
     gene            3488..4153
                     /locus_tag="VGR03_17650"
     CDS             3488..4153
                     /locus_tag="VGR03_17650"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEV1996163.1"
                     /translation="MGLLERVGTLIRANINDMVDRAEDPEKMIKQVILDMENQYLQVK
                     TQVAVSIADQHMLEKKWRENEDSGKDWMHKAETAVDKSHDDLARAALDRFQTSQRLAQ
                     SYREQVDDQRAQVETLKGALVKLEQKLDEAKSKRDLLLARHRRGIALGKAARAQTAMG
                     DNSKSATFDRLKDRVHHTEAVATAEVELLTDDVGEKLTRLDRDAEVDRLLVDLKARRG
                     LPG"
     gene            complement(4287..>5432)
                     /locus_tag="VGR03_17655"
     CDS             complement(4287..>5432)
                     /locus_tag="VGR03_17655"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1996164.1"
                     /translation="VVNDGSDDRTLDEMREEFRLRPVRAVYVPEMQSAPVRGLYRSEL
                     NASLLVVDKESGGSKADAVNAGLNAATSPYVCIVDADSVLERDSLLRIMVPIMADPKR
                     VVAVGGIIRVLNGSEIEGGHLKRVRLPRKSIEVIQVIEYLRAFLIGREAWGQGNMLTI
                     ISGAFGVFRTDLVRAVGGYRPKAIGEDFDLVARLHRHLLEKGVDYRIHFVPDPMCWTE
                     VPSDLQSLGRQRARWQKGLLDVLWPNRDMLFRPRYGRIGSFALPYLWLFELFAPVIEI
                     AGIITIVLAACLGVLSREFFLQFLLFGYAFATVISIGSVLQEEITYKRYNDWQDVTRL
                     VSYCFLEHFPYRQLHMIWRLQGLWQYFRGDVEWKPLKRKGLDSARAQ"
BASE COUNT         1227 a         1586 c         1585 g         1034 t
ORIGIN      
        1 gcatcggacg aaagcgtcgc ggctgccgat gtccgcctaa ttcttcccac ttccgactct
       61 ccttcgggca gtacatccgc atcttctgcc ggaagttcaa cgggcacgct ggccgaacgc
      121 acggaagcgt tcgagaggga agtgatgctc gcggaaattc ggcgccacaa cttccatatg
      181 acgaatgtag ctcgggcatt gggcctggag cgcagtcatc tctataagaa gtgccagcaa
      241 ctgggaatcg acctgcagtc cctccgcaag ccagaatagg caaccaatcc gggatatgcc
      301 tgtaagttgt tgaaaataga agcaattttg ttcttgacaa catagcgata tgtaatatta
      361 tatttgcaga ttgcaataca tgggtcctct atgaaacttg gcgacaaact ccgctccctc
      421 cgcgccgtcg aaggttcgct ccgcggattg ggccgttcga tgactcaaca ggaactagcc
      481 accgccatga agagtgagat cggccgcggt ttgagccagg cgtatctctc gcaaatcgag
      541 aatggcgccc ggccccatct gacacacaca acacgcgagc tgctggcgcg attcttccgc
      601 gtctatccag gttttctggt ggacgacccg gaaggctata cgccagggct tcagtctgaa
      661 ttgcgcgcca tcgacgcgaa gatcgattcc tggctttttg ccggcgccga acagttcacg
      721 gctgatccgg tgcttcagcg tgctcttcaa accatcgcgg agcatgaaga ctcgcgccgg
      781 gcgattttgc tcctggcgga aattcttcgg acgccggact tagcggagcg cctcggggaa
      841 gtcttgcatc cgggcaatca tcagaacggg ggacagaacg gcaacgctca tccgccgccg
      901 tctgctgctc tccgcaactg agccgtgtcg gtcccgaaag ggccaaggta gcatttaaca
      961 gggcctgcgt ctcgaattcc agctcgcgca taaagtgcgc tcgaggtgtg ccatggcgtg
     1021 gtctgacttt tatctcttct gcttcattgt cggcttctcg ctgagcgtgc tctcgtttct
     1081 cgccggcgcc gtccacatcc acctgccgtt ccgtctgcac ctgccgtttc aaggtggaca
     1141 tcacgctggt ggtctccact ccagcggcca cgcgcatgcc ggcggacatt cttcctggtt
     1201 caacacttcg acgctgcttg catttctcgc ctggtttggc ggcaccggct acattttgac
     1261 gaactattcg aatctcgtgg cgattgcttc gctgggattg gcgacgcttg ccgggctcgt
     1321 ctccgggtca attgttttca agttcatggt gaaactgatg cattcgagtg aagcgccact
     1381 caacgaagcg gattaccgcg tggaaggatc ggtctgcact gtaacgctgc cgattcgcgc
     1441 gaacggcacc ggcgaagtgg ttttcttgct tggcggcgtg cgccgcagcg cgggcgcacg
     1501 gaccgacgac ggcaagccaa tcgaaaaggg cgccgaggtg gtaatcgagc gtcatgaaaa
     1561 cggcatcgct tacgtgaaac gttgggacga attcacaagc tgatggtttg acagcgcgcg
     1621 tttacaggta gcgcaagtga aacaagtttc ggactgacga acgatgcaaa cagtccgtgc
     1681 agggcaggaa aagggaggtc tatatgtggg ggtttccact agttccagtc gtcgccggac
     1741 ttgccgttct gggtgtgatc ctggtgatgg ctacggtagc gaggatgtat cgcaaggtag
     1801 gcccgcacga ggcgctcatt atttacggca tgggcgggac acgcatcgtg aagggcagcg
     1861 gtgcggtcat tttccccatg attcaaacat gcaaggagct gtcgctggag ctgatgtcgt
     1921 ttgacgtggc tccgcagcag agtctctaca cgcaacaggg tgtggccgtc acggtggagg
     1981 ccgttgccca gatcaaggtg aagtccgatc ccgtatccat ccgcaccgca gcggagcagt
     2041 tcctgtccaa gcggccggaa cagcgcgagg gcttgattcg cctggtgatg gaaggccatc
     2101 tgcgcggcat catcggccag ctcacggtcg agcagatcgt gaaggagccg gaaatggtcg
     2161 cggaccgcat gcggtccacc aacgcgagcg acatgaacaa gatggggctg gaagtcgtca
     2221 gcttcacgct caaggaagtg cgcgacaaaa acgaatacat caccaacatg ggccgcccgg
     2281 acgtcgcccg catcaagcgc gatgcagaag tagccgcggc cgaagccgat cgcgacatcg
     2341 ccatcaaacg cgccgaagct acgcgcgcct cggccgtcgc aaaggctcaa gccgaccagg
     2401 agcgcgtcgc cgcagaaacg gcttcgctgg ccaagcaggc cgaagcggag cgcgacctgg
     2461 atctcaagag ggccagctat ctggagatga ccaagaaggc gcaggcaatg gccgacaaag
     2521 cctacgaaat cgagaccaac atcatgcagc agcaggtcat caccgaatcg gtgagggtgc
     2581 agcaggtgga acgcgaagct caagtgaaag tgcaggaagc ggaaatcgag cgccgcgaac
     2641 gagaactcat cgccaccgtg ctgaagccgg ccgaagtcga gcgccagcgc atcgaaacgc
     2701 tcgccagcgc cgaacgtcag cgctcgataa ccgaagccga aggccgcgcc tctgcgatcc
     2761 gcgcgcaggg cgaagccgaa gccgagatca tcttcaagaa aggcgaagcc gaagccaagg
     2821 ccatgaacgt caaggcggcg gcattccagg aatacaacca ggccgccgtg ctcgacaaac
     2881 tcatcagcgg actgccggaa atcgtgcgcg cgctcagcga gccgctcagc aaggtagaca
     2941 aggtgaccat cgtttccacg ggcaatggcg atgccgctgg tgcttacaag ctgaccgggg
     3001 acatcaccag gattgccgcg caagtacccg cgctcttcga agcgctctcc ggcatgcaga
     3061 tgtcggagct actcagcaaa gtgcggctga tcggcgacaa agcgccgaag ccagagccgc
     3121 cgcaagcgcc gattaagaag gcgtaacgtt cgcttccgtc ccgctgggtt tgtgaaaacc
     3181 catcagggcg cccaatggtc tcgggagatg gccatgagca tgaaagaaaa acttctattc
     3241 gtttggcttc tcgtggtggt ggcattcagt tttgtattcc accacttttg ggcaagcacg
     3301 atctgtgcgc ttggcagggt cgtcgcgctg aacctcgttt ctttgccagg ccttgcaaag
     3361 tttgctgcgg gaatatgcgg cgtgcacctc ttgccctgat tcagtgtgcg gggctggcag
     3421 gcgcgctcgg ttgtctttga atgttttgag tggttcgtat catttggttc tcgaaaggag
     3481 atttaccatg ggacttttag aacgagtagg cactttgatt cgcgcgaata tcaatgacat
     3541 ggtcgatcgc gcggaagatc ctgaaaaaat gatcaagcag gtgatcctcg acatggagaa
     3601 ccagtatctc caggtcaaga cccaggtggc ggtttccatc gccgaccagc acatgctgga
     3661 aaaaaagtgg cgcgaaaacg aggactccgg gaaggactgg atgcacaagg cggagactgc
     3721 cgtggacaag agccatgatg atttggcccg cgctgcgctc gaccgcttcc agacctcgca
     3781 acggctggcg caaagctacc gcgaacaagt ggacgaccag agagcgcagg ttgaaacgct
     3841 gaagggggcg ctcgtgaaac tcgaacaaaa actcgacgaa gcgaagtcca agcgcgatct
     3901 gcttcttgcg cggcatcggc gcggcattgc cctgggcaaa gcggcgcgcg cgcagaccgc
     3961 tatgggcgac aactccaaga gcgctacgtt cgaccggctc aaggaccgcg tgcatcacac
     4021 cgaagcggtc gccaccgccg aagtcgagct cctaacagac gatgtcggtg agaagctgac
     4081 gcgtctggat cgcgacgccg aggttgaccg cctgctcgta gatttgaagg cccgccgcgg
     4141 cctgcccggc tgatgcgcta tcgatcggca acgaacacgg agcggcaagg tgccggcaaa
     4201 atgcggaaaa gtagtcacgg attctcttga agctcatagc caagcgaaca agcggcagag
     4261 gagtcgccct tagcagcatt gcgtgtctat tgggctcgag ccgagtcgag ccccttgcgc
     4321 tttaagggtt tccattcgac gtcgccgcga aagtattgcc aaaggccctg cagcctccaa
     4381 atcatgtgca attgccggta aggaaaatgt tcgaggaagc agtagctgac cagccgtgtc
     4441 acgtcttgcc agtcgttgta ccgcttgtaa gtaatttctt cttgcaagac ggaaccgatg
     4501 gagatcaccg tcgcaaacgc gtagccgaac agaaggaact gcagaaaaaa ttcccggctc
     4561 aacacaccca aacaagccgc gagaacgatt gttataatcc cggcgatctc aattacgggg
     4621 gcgaagagct cgaacagcca caaataaggg agcgcaaagc taccgatcct gccgtaccgc
     4681 ggccggaaga gcatatcccg gttcggccag agcacatcca acaaaccctt ctgccagcgc
     4741 gctcgctgcc ttcctaggga ttgcaggtcc gacggaactt ccgtccagca catcggatca
     4801 gggacaaagt ggatgcggta gtccacgcct ttctccagca agtggcggtg caaacgcgcc
     4861 accaggtcga aatcttcacc aatcgccttc ggccgatagc cgccaacggc gcgcaccaga
     4921 tccgttcgga atacgccgaa agccccggaa ataatcgtaa gcatatttcc ctgtccccac
     4981 gcttccctgc cgatcaagaa tgcgcgcaga tactcgatga cctggatcac ttcaatgctc
     5041 ttgcgcggca aacgcacgcg ctttagatgc cctccttcaa tctccgatcc attcaaaacg
     5101 cggatgattc cacctaccgc cacgacgcgt ttcgggtccg ccatgatggg caccatgatt
     5161 cgcaagagcg agtcgcgctc gagcaccgaa tcggcatcca caatgcacac gtagggtgag
     5221 gtcgccgcat tcaaacccgc gttcaccgca tccgctttgc tgcctccgga ttctttatca
     5281 acgaccaaca gacttgcgtt cagctcgctg cggtacaaac cccgcaccgg cgcgctctgc
     5341 atttcgggca cgtacaccgc ccgtacgggc cgcagccgga actcttctcg catctcatcc
     5401 agagtgcggt cgtccgatcc atcgttgaca ac
//
LOCUS       DASYVO010000510        10806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_253139, whole genome shotgun sequence.
ACCESSION   DASYVO010000510 DASYVO010000000
VERSION     DASYVO010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 10806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10806
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253139"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VGR03_17660"
     CDS             complement(<1..352)
                     /locus_tag="VGR03_17660"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1996165.1"
                     /translation="MRLVLPRLYVILDASLIVSGELDKARTLAECGVRLVQYRGKNQS
                     SGQQLRMASQLAKFFERRGVTFLVNDRPDIALLAGAAGVHVGQEDLSVEDARKVLGPG
                     KWVGVSTHSLEQVRR"
     gene            complement(359..1708)
                     /gene="accC"
                     /locus_tag="VGR03_17665"
     CDS             complement(359..1708)
                     /gene="accC"
                     /locus_tag="VGR03_17665"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV1996166.1"
                     /translation="MFRKILVANRGEIALRVICACKELGISTVAVYSEADRNALHVRF
                     ADEAVCIGPARSSESYLNIPQVISAAEVTNVDAIHPGYGFLSENANFAKVCEASEITF
                     IGPKPEILELMGEKDRARREMKAAGVPTVPGSDGVIENAAQLAKEAARLGFPLIIKAS
                     AGGGGRGMRVVRKNEELSTAYETARSEAQQAFGVPGVYAERYIERPRHIEFQVLGDQH
                     GKVIHLGERECSIQRRHQKLLEESPSPAVDPRRRRELGARVVRAMEKVGYTNAGTVEF
                     LMDGDGSFYFIEMNARIQVEHPVTEQVTGVDLVKAQIRIAAGEKLEAAVGAVNSLGHA
                     IECRINAEDPETFVPCAGRITAYQAPGGTGVRVDSAAYDGAQIPPYYDSLIAKLITHG
                     RDREEAIARMRRALEMFVIEGIKTSIPLQRRILADPNFAAGRLDTGFIERISASEEK"
     gene            complement(1722..2252)
                     /gene="accB"
                     /locus_tag="VGR03_17670"
     CDS             complement(1722..2252)
                     /gene="accB"
                     /locus_tag="VGR03_17670"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV1996167.1"
                     /translation="MKPKKPKKNSASPSFANLEIEQIERLLRFMTENNLEEFEYCRGN
                     LRIRLRKPPAGTLGRTLAPEMIVVAPRASGEMAATPTAPGAAADVHGGDDLHLVTSPI
                     VGTFYGAPSPGADSFVKVGSHVESGQTLCIVEAMKLMNEIESDVSGEVIRVYVEGGQP
                     VEYGQPLFGIRPSRKK"
     gene            complement(2260..3375)
                     /locus_tag="VGR03_17675"
     CDS             complement(2260..3375)
                     /locus_tag="VGR03_17675"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEV1996168.1"
                     /translation="MEETSVSSVLFMSLPFIERRRAVRDGLRSGGLEAVVVTQAANRY
                     YLSGFTGEDGVLIVSGAGAALVTDGRFTTQAAEEARGVRIIRQKGPLQESVGRYLKSR
                     GWKRIGYDPASVSIEQFGRLRRAAGRHRTWHRLQGAIESLRARKDGSELAQMRKSAIL
                     AGEVLESVLKLLRPGVREFEIAAEIEYQIRRRGATGSAFPSIVAFGPRSALPHARPTS
                     RKLRKNELVVLDLGAILGHYCSDITRTLFVGRAPARIRGWYKAVVEAQQAAVSAVRAG
                     AECGEIDAAARKVLKGHRLDRYFVHSTGHGLGLEVHEEPRVARGQRQRLSPGNVITIE
                     PGVYVPGTGGIRIEDDVAVHLHSSEVLTRVRRDFIEL"
     gene            complement(3403..5421)
                     /gene="pilQ"
                     /locus_tag="VGR03_17680"
     CDS             complement(3403..5421)
                     /gene="pilQ"
                     /locus_tag="VGR03_17680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEV1996169.1"
                     /translation="MKTNWPAIQCGCLLLATLATGTGSMRAQAQESGRGAQGRANTAV
                     ISSVAVTQAAECASVRVVGEGHLDVLVTRIQDPERLVLEFAGARLAVQRTVIPSVSAP
                     VRRVRLGQYKPNVARVVIDLTSDAQYEISQEASAVVITFRTAAVPSREKPVSTTATRS
                     LVTDAGTRHANFRITPVVARPAAPAKARQLATVAIPKFELPRELTQPSVGLASFGGKP
                     AKPDGPGPRTVTNSGTRASGVPRPATATSEKYTGAPLSVNLKEVDLRDFFRLIHEISG
                     LNVVLDPAVHGSLTIVLDEVPWDQALDIVMQNNGLDKQLNGNVLRIATKETLKREAEG
                     QRDLVKAQAEAIELVTVTRVLSYAKAATMKDTLKKFLTARGEILSDDRSNQLIIRDIP
                     SVIPNVDNLIRQLDRKSQQVEIEARVISASRSFARDLGIQLGLSGTTTSGRSVFGGNQ
                     NAGVSPVVLPSGLGLPPPPPLVSTGGSPAGAMPLLVNLAASAATSGIFFGHRSPNFAV
                     DLFISAAEAKGVGKLLSKPKITTQNNEKATVKQGTKIPVQTTINNTVSVQFIDAVLKL
                     EVTPQVTADGTVYMDVLVENTSIDPGIPRVQGIPALDTQSAETKVTVADGGTVVIGGV
                     IITSQRVDYTEVPLVGSLPIIGHLFRRQNINNTSQELIFILTPRIIPG"
     gene            complement(5418..6077)
                     /locus_tag="VGR03_17685"
     CDS             complement(5418..6077)
                     /locus_tag="VGR03_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996170.1"
                     /translation="MRCAQLLLTGILFTVASPGVSGQPKPAGQTPGAPEPQASKAPQN
                     SQKAAAPAAKPGPSPSKPNTKAAAKSSTGPAKSAAKAPKAAAKKTAQGRTKSAASTTE
                     AAESVAKNARRDPFESLIGRQQRQGQVLSTVPGKPGLVISSLRLDGVVRTPDVMIAIV
                     TNPQQRTYFLREGDQLFDGRVEKISMDSVSFHEVGKDAFGKPVERQVNKRLYPSPGEQ
                     R"
     gene            complement(6094..6726)
                     /gene="pilO"
                     /locus_tag="VGR03_17690"
     CDS             complement(6094..6726)
                     /gene="pilO"
                     /locus_tag="VGR03_17690"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEV1996171.1"
                     /translation="MTNPFRDMSVFMQALVAVAIVVVLVALGVYLPVSPVAQTRVSLD
                     KAIQERGKLTQEVAQLQVYKSRYSELKQQMDALNKQLDTLKTIVPEEKELDEFMRLLQ
                     GAASASNVHIRRLTAQAVTPKETLYFEMPFEVEVDGPYFNVLDFFSRLSRLSRIINVG
                     DLAFHDTQKAGRYPLRPGTTVQGTFTAITFFTKPGETATAKGAAKPPGKP"
     gene            complement(6736..7371)
                     /locus_tag="VGR03_17695"
     CDS             complement(6736..7371)
                     /locus_tag="VGR03_17695"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEV1996172.1"
                     /translation="MIRINLLGQARPKAGRKHVPLGAALPVALLVAAFVFGAAILSLY
                     WYTLKKDLTEEQNHIKQLRAQKTDLEQIKAQVDSFDQQKKVLQAKVAVIEQLQRDRTG
                     GRELLDTLANTVTRTENLWLTSMTRKGNSLSIDGAAASINSVANFISQLKRSGYFQKV
                     EIKESKQDEGISAIETFAFQLTADIVPPQATKPAASAGPPATPPPAPAKKG"
     gene            complement(7374..8423)
                     /gene="pilM"
                     /locus_tag="VGR03_17700"
     CDS             complement(7374..8423)
                     /gene="pilM"
                     /locus_tag="VGR03_17700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEV1996173.1"
                     /translation="MFGMGKSSRQVVGLDIGSSSIKAVELKATKMGYELVSMGMEPLA
                     QDTVVDGAIMDAPQVANAISKIFDAQHIKTKNVATSVSGHSVIVKRVPLPLMSEEELY
                     DRIPSEASQHIPFDIADVNLSYQLLESMDSQMDVLLVAVKKEKILNHTNVLAQAGKTP
                     VVVDIDAFALQNCFELNYEPDASQTVALLNIGASVMNINIVRGGIPLFTRDVSVGGNQ
                     YTDALQKELDLSFDDAERLKRGEAIPSVSDEQKQQILRSVSDILTLEIQKTFDFFRAT
                     ASEENIQRIYMAGGTARVPGLGDLLREEFAMPVDELNPFRKVLTNPSRQLDEQILELS
                     PRLAIAGGLALRSFD"
     gene            complement(8457..8654)
                     /locus_tag="VGR03_17705"
     CDS             complement(8457..8654)
                     /locus_tag="VGR03_17705"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1996174.1"
                     /translation="MNETREVMNVRQASNYLGVSADTLYRYITEGEIPAFKLGNRWKL
                     RKTILDRWMERKMSQAHARRH"
     gene            complement(8948..10405)
                     /locus_tag="VGR03_17710"
     CDS             complement(8948..10405)
                     /locus_tag="VGR03_17710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HEV1996175.1"
                     /translation="MAYRDLREFVQRLEKEGELQRIRVEVDPVLEITEITHRVTRAGG
                     AALLFEKPKGSSYPLLINAFGSDRRMELAFDVEDLEEIAGRIRGFLDMQSPQGLLDKL
                     KMLPKLAELGSFFPKSVKSGPCKEIVKRGADVNLLEFPILKCWPQDAGRFITFPLVFT
                     KNPETGKRNVGMYRMQVYDERTTGMHWQTQKHGAEHFRRSREKDPAGRIPVAVAIGAD
                     PATSLAGMLPIPPDLDEMMFAGFLRREPVEMTACETCELEVPANAEIVLEGYVRLDEM
                     RVEGPFGDHTGFYSLEGDYPVFHVECVTHRRNPIYLTTIVGPPPQEDFFMGHAVERIF
                     LPVMRMQYPEIVDVAMPAEGIFHNLMIVAIRKSYPGHARKVMNAIWSLGQAMFTKVII
                     VVDHDVNVQDFREVTWKTLCALDPERDVQFVLGPTDTLDHAARMQDFGSKMGIDATRK
                     WRSEGFMRPWPDEIRMDEATRARIDALWASLGFKR"
BASE COUNT         1933 a         3438 c         3128 g         2307 t
ORIGIN      
        1 cgcggcgtac ctgctccaag ctgtgcgtcg agaccccaac ccatttgccc gggccgagga
       61 ctttccgcgc atcttccacg ctgagatctt cctgccccac atgcacgcct gcagctcccg
      121 cgagcagagc aatatccgga cggtcgttga caaggaaagt tacgccccgc cgttcaaaaa
      181 acttcgcgag ttgcgatgcc atccgcaact gttggccgga cgattgattc ttgccgcggt
      241 attggaccag gcgcacgcca cactccgcaa gagttcgggc cttatccagt tccccggaaa
      301 cgatcagcga tgcgtcgagt atgacataga gacggggaag cacaagtcgc atcgtcgcct
      361 atttttcctc cgaagcggaa attcgctcga tgaagccggt atccagccgg cccgcggcga
      421 agtttggatc ggccaggatg cggcgctgaa gggggatcga agtcttgatg ccctcgatca
      481 cgaacatttc caaggcgcgg cgcatcctcg cgatggcttc ctcgcggtct cgcccatgcg
      541 tgatcagctt tgcgatcagg gaatcgtagt acggcgggat ctgcgccccg tcgtaggccg
      601 ccgaatccac gcgaactccg gtgccccccg gcgcctggta ggccgtgatc cggcccgcgc
      661 agggcacaaa tgtctccggg tcctccgcat tgatgcggca ttcgatcgcg tgcccgaggg
      721 aattcaccgc ccctacggcg gcttccagtt tctcaccggc ggcaatccgg atttgcgctt
      781 tgaccaggtc caccccggtc acttgttccg tcaccgggtg ttccacctgg atgcgcgcat
      841 tcatctcgat gaaatagaac gatccgtcac cgtccatcag gaactccacg gtgccggcat
      901 ttgtgtagcc gaccttctcc atggcgcgca ccacccgggc gccgagttcc ctgcggcggc
      961 gtggatccac cgccggagaa ggcgactctt cgaggagctt ctggtggcgc cgctggatgc
     1021 tgcactcgcg ctcgcccagg tgaatcacct tcccgtgctg atcgccgaga acctggaatt
     1081 caatgtgccg cggccgttcg atgtaccgct ccgcatacac gcccggcacg ccgaaagcct
     1141 gctgggcctc gctgcgggcg gtttcgtagg cggttgacag ttcttcattc ttgcgcacca
     1201 cgcgcatccc gcgccccccg ccaccggccg aggccttgat gatcaacggg aagccaagcc
     1261 gcgccgcctc cttggcgagc tgcgccgcgt tctcaatcac tccgtcgctg ccgggaacag
     1321 tgggtacacc cgcggccttc atctcccggc gcgcgcggtc cttttctccc atcaattcga
     1381 ggatttcggg ttttggcccg atgaacgtga tctcggacgc ctcgcagacc tttgcgaaat
     1441 tggcgttttc ggagaggaac ccgtagccgg gatggatcgc atccacgttc gtgacttccg
     1501 ccgcgctgat cacctggggg atattcaggt agctctcgct gctgcgggcc ggcccgatgc
     1561 aaaccgcctc atccgcaaag cggacgtgca acgcattgcg gtccgcctcc gaatacaccg
     1621 ccaccgtgga gatgcccagt tctttgcagg cgcaaatgac ccgcagggcg atttccccgc
     1681 gattggcgac gagaattttg cggaacatga cgcgatgggg gctatttctt ccggctcgga
     1741 cgaatgccga acagcggctg gccatattcc accggctggc cgccttccac atagacccgg
     1801 atgacctcgc cacttacatc cgactcgatt tcgttcatca gcttcatggc ctctacgata
     1861 cacagcgtct gtccggattc cacgtgggag cctactttta cgaacgagtc cgcgcccggg
     1921 ctcggcgccc cataaaaggt gcctacgatc ggtgatgtga ccaggtgcag gtcgtctccg
     1981 ccatgtacgt ccgccgctgc tcctggagcc gttggagtgg ccgccatttc cccggaagcg
     2041 cgtggcgcca ccacgatcat ctcgggggcc aaggtccgtc ccagagttcc cgccggcggt
     2101 ttcctcaaac ggatacgcag gtttccgcgg caatactcaa actcttcgag gttgttctcc
     2161 gtcatgaagc ggaggagccg ctcgatctgt tcgatctcta gattggcgaa ggacgggctc
     2221 gctgaatttt tcttcggctt tttcggtttc atgcttgttt cagagctcga tgaaatcccg
     2281 ccggacgcgg gtcaacactt ccgaagaatg caagtggacg gcgacgtcat cttcgatgcg
     2341 aatgccccct gtgccgggaa cgtagacccc gggttcaatc gtaatgacat tacccggcga
     2401 gaggcgctgt ctctgcccgc gtgccacccg aggctcctcg tgaacctcca gcccgagccc
     2461 gtgacctgtg ctatgcacga agtagcggtc cagcctgtga cccttcagga ccttccttgc
     2521 agccgcgtca atttcgccac actccgcacc ggcccggacc gccgaaacgg ccgcctgctg
     2581 cgcctccaca accgccttgt accatcctcg gattcgcgct ggcgcacgtc ccacaaatag
     2641 cgtgcgggta atgtcgctgc aatagtggcc gagtatagca cccaggtcga ggacgaccaa
     2701 ttcatttttc cgtagcttcc ggctcgtcgg gcgggcatgc gggagcgcgg accgtgggcc
     2761 gaaggcaacg atggagggga aagcggaccc ggtagcgccg cggcgcctga tctggtattc
     2821 gatttctgcg gcaatctcga attcgcgcac acccggccgg agaagcttca ggacgctctc
     2881 caggacttct cccgcgagga tggctgactt gcgcatctgg gccagctctg agccgtcctt
     2941 gcgcgctctc aagctctcta ttgcgccctg taagcggtgc caggtccgat ggcggcccgc
     3001 ggcgcgccgc agcctcccga attgctcgat gcttaccgaa gccgggtcgt accctatccg
     3061 cttccaaccc ctcgacttaa gatagcgccc gaccgactct tgcagcggcc ctttctgccg
     3121 gataatccgc acgcccctgg cttcctccgc cgcttgcgtc gtgaacctcc catccgtgac
     3181 taacgccgcg cccgcccccg agacgatcag aaccccgtcc tcgcccgtaa atccgcttag
     3241 gtagtaacgg ttggcagcct gcgtgacaac cacggcttcc aggccaccgg accgtaggcc
     3301 atcgcggacg gctcgccgcc gctcgataaa aggtaagctc ataaacaaaa cagaggagac
     3361 cgaagtctcc tccattatgc cctcagatga tgccggagaa aactaacccg gaatgatacg
     3421 cggggtcagg atgaaaatca gctcttgaga ggtgttgttg atgttctggc gtctgaatag
     3481 atgcccgatg atcggcaggc tcccgacaag gggaacttcc gtgtagtcaa cccgctgcga
     3541 ggtgatgatg acccctccga tgacgaccgt gccgccgtcc gcgacggtga cctttgtctc
     3601 cgcggactgc gtatcgagag ccggaattcc ctgcacgcgc gggatccccg gatcaatcga
     3661 tgtattctcg accagaacgt ccatgtagac cgttccgtcc gccgtaacct gcggcgtcac
     3721 ttcgagcttc agcacggcgt cgataaactg caccgaaacc gtattgttga tcgttgtttg
     3781 aacggggatc ttcgtccctt gtttgacggt ggccttttca ttgttctgcg tcgtgatctt
     3841 cggcttcgaa agcagcttcc ctacgccttt cgcttcggcc gcgctgataa agaggtcaac
     3901 cgcgaagttc ggagagcggt gcccgaagaa tattccgctc gtcgctgcgg aggccgcaag
     3961 gttaaccagg agcggcatgg cgccggcagg cgatccacca gtcgacacca gcggcggcgg
     4021 cggagggagc ccgagcccgg aggggagaac gaccgggctg acgcccgcat tctggtttcc
     4081 gccgaagacg gagcggccgc tcgtggtggt acccgaaagg ccgagctgaa taccgaggtc
     4141 tcgcgcaaac gagcgcgagg cggaaataac gcgggcttcg atttccactt gctgcgattt
     4201 gcggtcgagc tgccggatca ggttgtccac atttgggatc accgacggaa tatcccggat
     4261 aatcagttgg ttggagcgat cgtcggagag aatttccccg cgggcggtga ggaacttctt
     4321 caacgtgtcc ttcatcgtgg cggccttcgc gtaactcagc acgcgggtga ccgtgaccag
     4381 ttcgatggct tccgcctggg ctttcaccag gtcgcgctga ccttcggctt ccctcttgag
     4441 tgtctctttg gttgcgatgc gcagcacatt cccgttcagt tgcttgtcca gtccgttgtt
     4501 ctgcataacg atgtccaagg cctggtccca cggcacctcg tcgagaacaa tcgtcagcga
     4561 tccatggact gcgggatcga gcacaacgtt tagtccgctg atttcgtgga tcaaccggaa
     4621 gaagtcccgg agatccacct ctttcagatt caccgatagg ggtgcgccgg tatacttctc
     4681 actcgtggcc gttgctggcc gtggtacgcc agaggcccta gttccggaat tcgtcacggt
     4741 ccgcggcccc ggaccatccg gcttcgccgg cttccctccg aacgaagcca gcccgacgga
     4801 gggctgggtc agttcgcgcg gcaactcgaa cttgggaatc gccacggtcg caagctggcg
     4861 agccttggcg ggggctgccg gacgggcgac cacgggggta atgcggaaat tcgcatgccg
     4921 cgttccagca tccgtcacca gagagcgggt tgcagtcgtg ctgacgggtt tctcgcggga
     4981 ggggactgcc gcagtgcgga aggttatgac cacggcggag gcttcctggg aaatctcgta
     5041 ctgcgcgtcg ctggtcaggt ctatgaccac gcgcgccaca ttgggtttgt actggcccaa
     5101 gcggactcgg cgcaccggtg ccgagacgct cggaatgacc gtcctttgca cggccagccg
     5161 cgcgcccgcg aactccagca cgaggcgttc gggatcttga atgcgcgtta ccagaacgtc
     5221 gagatgccct tcccccacca cgcgcacgct cgcgcattct gcggcctgcg taaccgctac
     5281 gctagagatg actgcggtgt tcgcccggcc ctgcgctccg cgcccagact cttgcgcctg
     5341 cgcccgcatc gagcccgtcc ccgtcgccag agtggctagg agcaggcagc cgcactgaat
     5401 ggccggccag ttcgtcttca tcgctgttct cctggacttg gataaagtct cttgttcacc
     5461 tgacgctcca cgggtttgcc gaaagcgtcc ttgcccactt cgtggaacga cacgctgtcc
     5521 atgctgatct tttccactcg gccatcgaac agttgatcgc cttcccgcag gaagtaggtg
     5581 cgttgctgcg gattcgttac gatggcgatc atcacgtccg gcgtgcgcac cacaccgtca
     5641 agccgcagcg aggagatcac gagtcccggc ttgccgggca ccgtcgacag cacctgccct
     5701 tgtctctgct ggcgcccaat cagcgattcg aacggatcgc gcctggcatt cttggctacg
     5761 ctctccgcag cctcggttgt cgaggcggcg gatttcgtcc ttccctgggc cgttttcttc
     5821 gcggcagcct taggcgcctt tgccgcagac tttgccggtc ccgttgagga cttcgccgcc
     5881 gctttcgtgt tcggcttcga aggagacggg ccgggcttcg cggccggagc cgcggccttt
     5941 tgcgaattct gcggtgcctt ggaggcttgc ggttctggag cgcccggcgt ttggccggct
     6001 ggtttgggct gcccagaaac tcccggtgaa gccaccgtga acaagattcc tgtcaaaagt
     6061 agttgtgcgc aacgcatagt ttgctcaacc cgcctacggt ttccctggcg gcttcgcggc
     6121 cccctttgcg gtcgctgtct cccccggttt tgtgaaaaac gtaattgccg taaatgtccc
     6181 ttgcaccgtc gttcccggcc gcaacgggta acgccccgcc ttctgtgtat cgtgaaacgc
     6241 caggtcgccc acgttgatga ttcgcgagag gcggctcaac cggctgaaaa agtccaagac
     6301 attgaagtaa ggcccgtcga cctccacctc gaacggcatt tcgaaataaa gcgtttcttt
     6361 cggcgtaacc gcctgggccg tgagcctgcg gatatgcacg ttcgaagcac tcgcagcccc
     6421 ctgaagcagc cgcatgaact cgtccaattc cttctcttcc ggcacgatcg tcttcagggt
     6481 gtccagttgt ttgttgagcg catccatctg ttgcttcaat tcgctgtacc ggctcttgta
     6541 aacttgcagc tgcgccactt cttgagtcag cttgccccgt tcctggatgg ccttgtccag
     6601 ggacacgcgt gtctgcgcca ccggcgaaac gggcagatag acgcccagtg caacgagaac
     6661 cacgacgatc gccaccgcca ccagcgcctg catgaacacc gacatgtctc ggaacggatt
     6721 cgtcatgtgc ggtctctacc ctttcttagc cggagccggc ggcggagtag cgggcggacc
     6781 cgcggaagcc gctggcttcg tcgcctgcgg cggaacgatg tcggcggtga gctgaaaggc
     6841 aaacgtctcg atcgcggaga tcccctcgtc ctgcttcgac tctttgatct cgaccttctg
     6901 gaagtagcct gagcgcttca attgcgagat gaagttcgcc accgaattga tcgacgcggc
     6961 tgcgccgtcg atcgaaagcg agtttccctt gcgggtcatc gatgtcagcc agagattctc
     7021 cgtgcgagtt accgtattgg cgagcgtgtc gagcaattcc cggcccccgg tgcggtcccg
     7081 ctgtagctgt tcgatcaccg ccaccttcgc ctgcaagacc tttttctgct ggtcgaacga
     7141 gtccacttgg gctttgatct gttccaggtc cgttttctgc gccctcagtt gcttgatgtg
     7201 attctgttcc tccgtcaggt ccttcttcag cgtgtaccag tagagagaca gaatggccgc
     7261 cccgaaaacg aaggccgcaa ccaggagcgc cactggcaga gccgcgccga gcggcacgtg
     7321 ttttcgcccg gccttcggcc gcgcttgtcc gagaaggttt atgcggatca tggctagtca
     7381 aagctcctca gggcaagccc cccggcgatt gccagccgtg gagataactc caggatttgc
     7441 tcatccagct gccgcgaagg attcgtcagc actttgcgga agggattcaa ttcgtcgacc
     7501 ggcatggcga actcttcccg cagcaagtcc cccaggcccg gcacccgggc cgtgccgcct
     7561 gccatgtaaa tgcgctggat gttctcctcg ctggcggtgg cccggaagaa atcgaaggtt
     7621 ttctggattt cgagcgtcag gatgtccgaa acactgcgca gaatctgctg tttctgttcg
     7681 tcggatacgc tgggaatcgc ctcgccccgc ttcagccgtt cggcgtcgtc gaagctcagg
     7741 tccagctcct tttgcagtgc gtccgtgtac tgattgccgc ctacggagac gtctcgcgtg
     7801 aacagcggta ttccgccccg gacgatgttg atgttcatca cgctggcgcc gatgttcaac
     7861 agggccacgg tctggctcgc atccggttcg taattcaatt caaagcagtt ctgcagagcg
     7921 aacgcgtcaa tgtccaccac caccggtgtc ttgcccgcct gtgccagcac gttagtgtgg
     7981 ttcaggatct tctccttctt gaccgccacc agcagcacat ccatctgcga gtccatggac
     8041 tccagcagtt ggtagctgag gttgacgtcg gcgatgtcga aaggaatgtg ctggctcgct
     8101 tccgacggaa tccggtcgta cagctcttcc tcgctcatca gcggaagcgg gacgcgcttg
     8161 acgatcaccg aatgccccga tacggacgtg gcgacgtttt tcgtcttgat gtgctgcgca
     8221 tcgaatatct tgctgatcgc gttggcgacc tggggcgcat ccataatcgc gccgtccacc
     8281 accgtgtcct gagccagtgg ctccatcccc atgctcacca gttcgtagcc catcttcgtg
     8341 gccttcagct ccaccgcctt gatggagctg gaaccaatgt ccaatcccac tacctgtctg
     8401 ctcgatttcc ccatgccgaa cattttgcgg cctctttccc tgtccctggc gccggcttag
     8461 tggcgcctcg cgtgcgcctg gctcatcttg cgttccatcc agcggtccaa gatcgtctta
     8521 cgaagtttcc aacgattacc cagtttgaac gcgggaattt cgccctccgt gatgtaacgg
     8581 tacagggtgt ccgcggaaac ccccagatag ttggacgcct gccgtacatt cattacttca
     8641 cgcgtctcgt tcacgagaaa cgctgcctcc gggcgttcat tgccagcgag cagttcgatt
     8701 ttgtgatgcg ccggcgcacc cgctgctttt caacgcaccc cgccggtccg cgccgtgttt
     8761 cggttatgac actgaagaaa gccgggggtc aagagattgc ttacctgagt tgtcctaaga
     8821 attcaggtat tcatgggcag attgcccgca gtctttggca cgctggccca catcttgcgt
     8881 tatggaaggt acaaaaccgc aatatattgt attttacgcc gatttcccgg gctgtacgga
     8941 aggacggtta tcgtttgaag ccaagcgacg cccaaagggc gtcgatgcgc gcccgcgtcg
     9001 cttcgtccat gcggatttcg tcgggccagg gacgcatgaa accttccgac cgccacttcc
     9061 tcgtggcgtc gatccccatc ttggatccga agtcctgcat ccgcgcggcg tggtccaggg
     9121 tatcggtcgg gccaagcacg aactggacgt cccgctccgg atcgagagcg cacagcgtct
     9181 tccaggtgac ctctcggaag tcctgcacgt tgacgtcgtg gtccacgacg atgatcacct
     9241 tggtgaacat ggcttgtccc agcgaccaga tcgcgttcat gaccttccgc gcgtgccccg
     9301 ggtaggactt gcggatggcg acgatcatca agttgtgaaa gatgccttcg gcgggcatgg
     9361 ccacatccac gatttccggg tactgcatgc gcatcaccgg caggaagatg cgctccacgg
     9421 cgtgccccat gaagaaatcc tcctgcggcg gcgggcccac gatggtcgtg agataaatcg
     9481 ggtttcgacg gtgggtcacg cactcgacgt gaaagaccgg atagtcccct tccagcgaat
     9541 agaaccctgt gtggtcgccg aagggtccct cgacgcgcat ctcatcgagc cggacatagc
     9601 cctccagaac gatttccgcg ttcgcgggaa cctccaactc gcaggtctcg cacgccgtca
     9661 tctccaccgg ttcccgccgc aaaaaccccg cgaacatcat ctcgtccagg tccggcggaa
     9721 tcggcagcat cccggccagc gaggtggccg ggtcggcgcc gatggccacc gcgaccggga
     9781 tcctccccgc cggatctttt tcacggctgc ggcggaagtg ctctgcgccg tgcttctgcg
     9841 tctgccagtg cattccagtg gtacgttcgt catacacctg catccggtac attcccacat
     9901 tgcgtttgcc ggtttccgga ttcttcgtaa agaccagggg aaacgtgata aaccgcccgg
     9961 cgtcctgcgg ccagcatttc aggatgggaa actccagcaa attcacatcg gcgccgcgct
    10021 tcacgatctc cttgcacggc cctgacttca cgctcttcgg aaaaaacgat cccagctccg
    10081 cgagcttcgg caacatcttc agcttgtcga gcagcccctg cggggattgc atgtccagga
    10141 atccgcggat gcgcccggca atttcttcga gatcctccac gtcgaaggcc agctccatcc
    10201 ggcggtcgct gccgaaggca ttgatcaaga ggggatagct cgaccccttg ggcttttcga
    10261 agagcagcgc ggctccgccg gcgcgggtga cccggtgcgt gatttccgta atctcaagaa
    10321 ccgggtccac ctcaactcga atccgttgca gctcgccttc tttctcgagt ctctgcacga
    10381 actcccgcag gtcgcgatag gccacaagtc ctccactggg ctcaagaact gggttgcctg
    10441 aacgtcagac tctagcacgg acgttccctc ggttgcgact tggcttgcca acgtcgaagt
    10501 cgcgcgcgag ggattgcaac ggttgtgagc gacaagaaaa agttgcgagt gcttcccgcc
    10561 gctgcggact tgacgacgct ggttgcagga gagttctagt actgaggaag ggggattttt
    10621 taggggcatt ttcgggctct tttttgatgt tttccccatg tttcacttgt caaaatgatt
    10681 ttacttgaca aatctttttg acaagtagtt ttagaagctt gaagaccctc gaaaactcag
    10741 gacaagctgg ggctggaatc gaaaagatgg gcgttggagg ttcgaggcca ggcgttggac
    10801 ggaggg
//
LOCUS       DASYVO010000511         8585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_222355, whole genome shotgun sequence.
ACCESSION   DASYVO010000511 DASYVO010000000
VERSION     DASYVO010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8585
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222355"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="VGR03_17715"
     CDS             complement(<1..1042)
                     /locus_tag="VGR03_17715"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1996176.1"
                     /translation="MVNIFRGFGAVFYKEALHVRRDAGTLFFSLTMPLLQMFLLGFGV
                     DTNIRQINTAVYNADGHRESRELIDRLKNSDTFHILRYVENDHDLNDLIVAGKARVGI
                     KIPVDYSDRLLHNMSAQVLVLIDGSDSSVAGQAINVTTAIGLDESLRRVLKDRSAFAV
                     EMRPKLLFNPDSRSPNFFLPGLTAIMLLNVTTFLTAFSIVREKERGTLEQLFVTPVRP
                     FGLLLGKLLPYLGIGFFELCLILFFMRFVFRVPIHGSVLLLAILSLPYLFAALSLGIL
                     ISSKANSQAEAMQLAFLPILPSVFFSGYIFPRETMPALFYPISYLIPATYFINITRGI
                     ILRGAGIKHLWTD"
     gene            complement(1035..2018)
                     /locus_tag="VGR03_17720"
     CDS             complement(1035..2018)
                     /locus_tag="VGR03_17720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1996177.1"
                     /translation="MADATKNPAEAAISAEHLVRRFGNFTAVNDVSFRVEKGEIFGFL
                     GPNGSGKTTVIKMLTGLLPFSGGSALVEGLDVATDSESVRERIGYMSQNFSLYYDLTV
                     AENLQFYGRIYSLEPARLKRRIEEIVQLNGFAPYLNRLAGQLSGGWKQRLALGCAMLH
                     EPKLLFLDEPTAGIDPVARRQLWDLLFELSGHGITFFVTTHYMDEAERCSHVAYIYYG
                     KLIADGTPNSLRELPDVQPPGTMRVEITTPEVTRALRFARQIPGIRSATIFGQSIHAL
                     IEDHFNLDDLREQLLKNGIAVAEIRPLAPSLEDVFVELTYKHQALQEAVRG"
     gene            complement(2023..3120)
                     /locus_tag="VGR03_17725"
     CDS             complement(2023..3120)
                     /locus_tag="VGR03_17725"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV1996178.1"
                     /translation="MNRKHILILLAATAALAATGLYADWFHRDTALQGSGTVEARNIR
                     VGSKIGGRIDKVLVREGDSVQPGQVLITFDDKELQASLEQSRANDEKARRGYRPEEIA
                     EARAVAAQAKAEYELKKNGYRQEDIGTAQADLDRAKADEIRTHLDFDRYDALARKDLV
                     SKQQRDTAEANWKVAVAQQQSAAHKLDELQRGYRPEEIASAEAHYHQAQANMEKLERG
                     NRREDVDLAKAAYSYDEARFRERQVIAPSAAIVEVLDVRPGDLIAPNTPVATLLEKDQ
                     IYVRIYIPETEFGRLKLGQKAEVRVDSFPKTVFEGVLEQINQQAEFLPRNVQTREERV
                     HQVFGVKIRISDPEGHVLAGMAADVKLKEGN"
     gene            complement(3125..3820)
                     /locus_tag="VGR03_17730"
     CDS             complement(3125..3820)
                     /locus_tag="VGR03_17730"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1996179.1"
                     /translation="MARLPKPIPRTDLSGSVSTSDRRQRRSADIRERLFRAALDLFAQ
                     KGFAEATVEDITEAADVGKGTFFNYFPSKDHILLAFGDMQLSKLEAAIEVARRTAEPM
                     PEFLRSLALRMTQEPTRNPAIIRALLQAYLSTTPVREAMMDLQKRVHALHSQMIQLGQ
                     DRGEIRNDLPAAEIAHVFRQTIFGTLLIWSLYGDATLHSRIETAFNLLWSGLAPRGNS
                     TPPISVPFFTSGD"
     gene            complement(3989..4705)
                     /locus_tag="VGR03_17735"
     CDS             complement(3989..4705)
                     /locus_tag="VGR03_17735"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEV1996180.1"
                     /translation="MIKRMISSLMALTLLALPLLAAGDKKETERLENCGMVLKEILDI
                     PDDIPQDLLDKAECVIVYPSVLKAAFVVGGSYGRGAMTCRTGEHFTGPWSAPAMMALE
                     GASIGFQLGGQATDFVLLVMNPRGATSILSSKVKLGADASAAAGPKGRNAEASTDLTL
                     RAEVLTYSRARGLFAGISLTGSTVRPDNDANERIYDKKVDATDIIRKGAVAVPPAAQK
                     MVALLNQKSPKNTSDPESLK"
     gene            4901..6520
                     /gene="dacB"
                     /locus_tag="VGR03_17740"
     CDS             4901..6520
                     /gene="dacB"
                     /locus_tag="VGR03_17740"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEV1996181.1"
                     /translation="MRIEKILQRWAAVAVASAVWSFGIGAPRLGAQSKAKTEPACCGA
                     KKLSAASKKATARFATRAEALLGTMPTNKGEWGLLIVDAESGETLYEQNADKYFVPAS
                     NMKLFTTALALAKLGPEYRFHTTLETRGAISSEGVLSGDLALVGRGDPNLSNRKFPYE
                     LKEEFDGPPEKALVELADALVAKGVKEISGDVIGDDSYFPRERYPNGWEIDDIVWEYG
                     AAISAIVVDDNTVALVLTPGEQAGGPVQAAVTPATPDFTVENDVITSAAEVKSDLTLT
                     REPGANLVVVKGSLPAKSAPRKLVLAIEEPAQHAAATLKRLLEERGVRVAGVARARHV
                     RAELGGDPVVLAEHVSVPLGDAVKLINKISQNLHAEMLLRTVARQSGVWASPDELMKA
                     PADFYAAAGITPGDVIQTDASGLSRHDLVTPRAIVTLLSFAQKQPWFGPYSASLPVTG
                     LDGTLEDRMKNTLAAGRIHAKTGSVEHVRTLSGFAETPGGRRVIFSFLSNNQGGKSHE
                     AADALTGLCVAMLEEFNTTPPKRGLEHKHPQ"
     gene            6536..7093
                     /locus_tag="VGR03_17745"
     CDS             6536..7093
                     /locus_tag="VGR03_17745"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEV1996182.1"
                     /translation="MGKEIIVTVFGSSRPREGNADYEEARILGRALAKHGFSVCSGGY
                     GGVMEAVSRGAKEAGGKTCGVTAKFFKAAKLNPWIDVEVRMKTWEERLFELIRRADGF
                     VACKGGTGTLVELAVVWEMLNKSVMAGKPFAVLGGFWQPVLDRVREVELGHPAPWGEA
                     NGRLVYVATTPGDVASHLTTKLKQI"
     gene            7096..>8585
                     /locus_tag="VGR03_17750"
     CDS             7096..>8585
                     /locus_tag="VGR03_17750"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996183.1"
                     /translation="MSRTAQDVLEFDKLRELLRLRTTCAPGRRAVNGLEPGRERAALE
                     SAFALIREAGEWLRAGSELGFGALADPQGWLERIEGPGMALGPGEFLDAASLLETAGW
                     LRQQFREEAAKFPLLAARAAALGDFRDVHAAIRRCVLPNGEISDDASATLRRIRASIT
                     ETRDTIQKNLKQILRARNAEAGEDYVTLRNDRFVIPVRAEHRRSLPGVVHGASATGQT
                     VFMEPFETVESNNQLVQLAEDEAAEILRILRELTEKLQMIRGPLLAAAGTIAELDSVF
                     ARARFARDFDAAMPEFSGTNELRLEGARHPVLEDKLRKENRAIVPMTLTLGGEERVLV
                     ISGPNTGGKTVVLKTTGMAALAAQSGIPVAAQRAVLPLFDRVLVDIGDEQSIAADLST
                     FSSHMLNLKTMLEAATPNSLVLVDEMGTGTAPEEGAALAVALLDEFRAKHCIVLATTH
                     HDRLKTYASTTPGVVNAAVEFDDVNLRPTYRLMVGVPGGSSGIAIA"
BASE COUNT         1785 a         2090 c         3046 g         1664 t
ORIGIN      
        1 cgtcggtcca gagatgcttg atgcctgcgc cacgaaggat gatgccgcgg gtgatgttga
       61 tgaagtacgt ggcggggatg aggtagctga tggggtagaa gagggcgggc atggtctcgc
      121 gggggaaaat gtagccggag aaaaagacgc tgggaagaat gggcagaaaa gcgagctgca
      181 tggcttccgc ttgggaattt gctttgctgg agatgagaat tccgagcgag agtgcggcga
      241 agagataggg caacgaaaga atcgcgagca gcaggacgct cccgtgaatg ggcacgcgga
      301 agacgaagcg catgaagaaa agaatgagac agagctcgaa gaagccgatg ccgaggtagg
      361 ggagtaattt gccgagaaga aggccaaagg gacggaccgg cgtgacgaag agctgctcga
      421 gagtgccgcg ttctttttcg cggacgatgg aaaaggcggt aagaaaggtg gtgacgttga
      481 ggagcatgat ggcggtgagg ccgggcagaa agaaattggg cgagcgcgag tcgggattga
      541 agagcagctt ggggcgcatt tcgacggcaa aggcgctgcg gtccttcaga acgcggcgaa
      601 gagattcatc gaggccgatg gcggttgtga cgttaatggc ctggccggcg acggaggagt
      661 ccgagccgtc gatgaggacg aggacctggg cgctcatgtt atggagaagg cgatcggaat
      721 aatcgacggg aatcttgatg ccgacgcgcg ctttgccggc gacgatgaga tcgttcaggt
      781 cgtggtcgtt ttcgacgtag cggaggatgt gaaaggtgtc ggagtttttc aggcggtcga
      841 tgagctcgcg gctttcgcgg tggccgtcgg cgttgtaaac ggcggtattg atctggcgaa
      901 tgttggtgtc aacaccgaag ccgaggagaa acatctggag gagcggcatg gtgagggaga
      961 agaagagagt gcccgcatcg cggcgcacat gaagcgcttc cttataaaac acggcgccga
     1021 aaccgcgaaa gatgttaacc acggacggcc tcctgcagag cttgatgctt gtaggtgagt
     1081 tcgacaaaga cgtcttcgag gcttggtgcg agggggcgaa tttcggcgac ggcgatgcca
     1141 ttcttcagga gttgttcgcg gagatcgtcg aggttgaaat gatcttcgat gagcgcatgg
     1201 atggactggc cgaaaatggt agcgctgcgg attccgggaa tctggcgggc gaaacggagc
     1261 gcgcgcgtga cttccggcgt ggtgatttca acgcgcatgg tgcctggagg ctgaacgtcc
     1321 gggagttcgc ggagcgaatt gggggtgcca tcggcgatga gcttgccgta ataaatgtag
     1381 gcgacgtggc tgcagcgttc ggcttcgtcc atgtagtggg tggtgacgaa gaacgtgatg
     1441 ccgtgaccgg aaagctcgaa gagcagatcc cagagctggc ggcgcgcgac ggggtcgatc
     1501 ccggcggtgg gctcgtcgag gaaaagaagt ttcggctcgt gcaacatggc gcagccgaga
     1561 gcgaggcgct gcttccagcc accggaaagt tgcccggcca ggcggttcag atagggtgcg
     1621 aagccgttga gctggacaat ttcttcgatg cgtcgcttga ggcgagcggg ctcgaggctg
     1681 taaatgcggc cgtagaactg caagttttcc gcgacggtca aatcgtagta gaggctgaag
     1741 ttttgcgaca tgtagccgat gcgttcgcgg acggattccg aatccgtggc gacgtcgagg
     1801 ccctcgacaa gggcggagcc gccgctgaac ggcaagagtc cggtaagcat tttgatgacg
     1861 gtggtttttc cgctgccgtt ggggccgagg aagccgaaga tttcgccttt ttcgacccgg
     1921 aagctgacat cgttgacggc ggtgaagttg ccgaaacgcc tgacaagatg ttcggcggaa
     1981 atggcagctt cggcaggatt tttggtcgcg tctgccatca actcaatttc cctctttgag
     2041 tttcacatca gcagccatac cggcaagcac gtggccttct gggtcactga tgcgaatctt
     2101 tacgccgaaa acctggtgca cgcgttcctc gcgggtctgg acgttgcgag gtaggaattc
     2161 agcctgctga ttaatctgtt cgaggacccc ttcgaaaact gttttaggaa aggagtcgac
     2221 tctgacttcg gccttctgcc cgagcttgag gcggccaaat tcggtttcgg gaatgtagat
     2281 acgaacgtag atttgatcct tttcgagaag agtggcgacg ggcgtattcg gtgcgatgag
     2341 atcgcccggg cggacgtcga gaacctcgac gatcgcggcg gaaggagcga tgacctggcg
     2401 ctcgcggaag cgggcttcgt cgtaagagta ggctgctttg gcgaggtcga cgtcttcgcg
     2461 acggttgccg cgctcgagtt tttccatgtt ggcctgtgcc tgatggtagt gcgcttcggc
     2521 agaggcgatt tcctcgggac ggtagccgcg ctggagttca tcgagcttgt gcgcggcact
     2581 ctgttgctgg gcgacggcga ctttccaatt ggcttcggcg gtatcgcgct gttgctttga
     2641 gacgagatct tttcttgcga gtgcgtcgta gcggtcgaaa tccagatgag tgcggatttc
     2701 gtcggccttc gcgcgatcga gatcggcttg tgcggtaccg atgtcttcct ggcgatagcc
     2761 gttcttcttc agctcgtact cggctttggc ctgagccgcg acggcgcggg cttccgcgat
     2821 ttcttcggga cggtagccgc gccgggcttt ttcgtcgttg gcacgggact gctcgaggga
     2881 agcttgcaat tccttgtcgt caaaggtgat caacacctgg cccggctgga cggaatcgcc
     2941 ttcgcggacc agaaccttgt cgatgcggcc gccgattttg gagccgacgc ggatgttgcg
     3001 ggcttcgacg gtgccggagc cttgcagggc ggtgtcgcgg tggaaccagt cggcatagag
     3061 tccagtggcg gccagcgcgg cggtggcggc aagaagaatc aggatgtgct tgcggttcat
     3121 gaaactagtc tcctgaagtg aagaaaggga ctgatattgg cggcgtcgaa ttgccacgag
     3181 gcgccaagcc ggaccagagc agattgaacg cggtttcgat ccgggaatgc aaagtggcat
     3241 cgccgtagag cgaccagatt agaagcgtgc cgaaaatggt ttggcgaaaa acgtgggcga
     3301 tctccgcggc cggaaggtcg ttgcggattt cgccgcggtc ctgaccgagc tgaatcatct
     3361 gactgtgaag ggcatgtacg cgtttttgca agtccatcat ggcctcacga accggagtcg
     3421 ttgaaagata ggcctgaaga agcgcgcgga tgatggcggg gttgcgagtg ggctcctggg
     3481 tcatgcggag agcgagcgaa cgcaggaatt caggcatggg ctcggccgtg cgacgagcca
     3541 cttcgatggc tgcttccagc ttactaagtt gcatgtctcc gaaggccaac agaatgtggt
     3601 ctttgctcgg gaaatagttg aaaaaagtgc ctttgccgac gtcggccgcc tcagtgatgt
     3661 cctcgacagt tgcctcggca aatccctttt gcgcgaatag atcgagggcg gcgcggaaaa
     3721 gacgctcgcg gatgtcggcg ctccggcgct ggcgacggtc ggaggtggag actgagcctg
     3781 agagatcagt gcggggaatg ggttttggta gacgggccat aatgaccaat gctcataaat
     3841 gactataggt catattggac tagatgtcaa gggcatttgc tcttagacca aaaaggaata
     3901 gttgccggag gcgatgcgcg ggagatagaa actagaacac cccggtttga gccggggtgt
     3961 cctgggaaag gccgtaggtt ttgagctact acttgagaga ttcggggtcc gaggtgttct
     4021 tcggagactt ctggtttagc aatgccacca tcttttgggc agccggagga acagcgacgg
     4081 ctcccttgcg gataatgtct gtagcatcaa ctttcttgtc gtagatgcgc tcgtttgcgt
     4141 cgttgtctgg ccggacggtg gagcccgtca aagagatgcc agcgaagagt cctcgagcac
     4201 gcgagtatgt aaggacttca gcgcggagcg tcaggtcggt ggaagcctcg gcgttgcgtc
     4261 ccttcgggcc agcggcggcg gaagcatccg caccgagctt gaccttgctg ctaagaatcg
     4321 aagttgctcc gcgcggattc atgaccaaga gcacaaaatc ggtggcctgt ccgccgagct
     4381 ggaagccgat gctggcgccc tcgagggcca tcatggccgg tgcgctccag gggccggtga
     4441 aatgctcgcc ggtgcggcag gtcatggcac cacggccgta gctgccgcca acgacgaagg
     4501 cagcctttag gacggacgga tagacgatga cgcattccgc tttatcgagc agatcctgag
     4561 gaatgtcatc ggggatgtcg agtatttcct tgagaaccat gccgcaattt tccaggcgtt
     4621 cggtttcctt tttatccccc gcggccaaaa gcggcagtgc aaggagcgtc aacgccatca
     4681 gagaagagat cattcgctta atcacagttc acctcgtcat agtcttctgg gctagagcca
     4741 tcatagagtg cccgttggct gggcgcaacc tcaggccagg gaaaggggcc tgggagacag
     4801 gcttgaggca gggatcagtt ccataaataa gacgcgcggg agacggaatg cgttgccttt
     4861 cgactatact tttctagaac gacttgggcg cgagtgaaaa atgagaatcg agaaaatcct
     4921 gcagcgatgg gcggcggtgg ctgtggcgag cgccgtgtgg tccttcggaa ttggcgcgcc
     4981 acgcctgggc gcacaatcga aagcaaagac agagccggcg tgctgtggcg caaagaaact
     5041 gagtgcggcc tcgaagaagg ccaccgcgag atttgcaacg cgtgcggaag cattgctcgg
     5101 aacaatgccc acgaacaagg gtgagtgggg actgctcatt gtggatgccg agagcggcga
     5161 gaccctgtac gagcagaacg cggataagta tttcgtgccg gcttctaaca tgaagctgtt
     5221 caccacggcg ctggctttgg cgaaactggg gccggaatac cgattccata cgacgttgga
     5281 gacgcggggg gcgatttcga gcgagggagt gctgagtgga gatctcgcgc tggtggggcg
     5341 tggcgatccg aatctatcga acagaaaatt cccgtacgaa ctgaaagagg agtttgatgg
     5401 tccgccggag aaggcgctcg tggagcttgc ggacgcgctg gtggccaagg gagtcaaaga
     5461 aatttcggga gatgtgatcg gagacgacag ttattttccg cgggagcgct atccgaatgg
     5521 ctgggaaatc gacgacatcg tgtgggagta cggcgccgcc atctccgcga tcgtggtgga
     5581 cgacaacacg gtagcgctgg tgctcacgcc tggcgaacag gccggtggtc cggtgcaggc
     5641 ggcggtgact cccgcgacgc cggatttcac ggttgagaat gatgtgatca cttccgccgc
     5701 ggaagtgaaa tcggatctga cgctcacgcg ggagccgggc gcgaatctcg tggtggtaaa
     5761 gggcagcctg ccggcgaaga gcgctccgcg gaaactggtt ctggcgattg aagagcccgc
     5821 gcagcatgcg gcggctacat tgaagcggct gctggaggaa cgcggagtaa gggtagcggg
     5881 cgtcgcgaga gcccggcacg ttcgtgcgga gctcggtggc gatccggttg tcctggcgga
     5941 gcacgtttcc gtgccgctgg gtgacgcagt gaaactgatc aacaagatca gccagaacct
     6001 gcacgcggaa atgctgttgc ggacggtggc gcgacagagc ggggtgtggg cctcgccgga
     6061 cgagttgatg aaagctccgg cggatttcta cgcggcggcg ggaatcacgc cgggcgacgt
     6121 gattcaaacg gacgcctcgg gattgtcgcg gcacgatttg gtgaccccgc gagccattgt
     6181 gacgttgttg agctttgcac agaagcagcc ctggttcggg ccgtattctg cgtcgctccc
     6241 ggtgacggga ttagacggca cgctggaaga ccgcatgaaa aacacgctgg cggcggggcg
     6301 gattcacgcg aagacgggct cggtcgaaca tgtgaggaca ctttcgggtt ttgcagagac
     6361 gccgggcggg cggcgcgtga tcttttcgtt cttgagcaac aatcagggcg ggaagagtca
     6421 cgaagcggcg gatgctttga ccgggttgtg cgtggcaatg ctcgaggagt tcaatacgac
     6481 gccgccgaag cgcgggttgg aacacaaaca cccgcagtga gatcgaggat tgcaaatggg
     6541 gaaagaaatc atcgtcaccg tgtttgggag ttcgcggccg cgcgaaggta acgcggacta
     6601 cgaagaggcg cgaatcctgg ggcgagcttt ggcaaagcac ggtttctctg tttgtagcgg
     6661 cggttacggc ggcgtgatgg aagcggtgtc ccgcggcgcg aaagaagccg gcggaaaaac
     6721 gtgcggcgtg acggcgaaat tttttaaggc cgcgaagctg aatccctgga ttgacgtgga
     6781 agtgcggatg aagacctggg aagagagact ctttgagctg atccgacgcg cggacggttt
     6841 tgtggcgtgc aaaggcggga cgggaacact cgtggagctc gcagttgtgt gggagatgct
     6901 gaacaagtcg gtgatggccg ggaaaccgtt cgcggtgctc ggaggtttct ggcagccggt
     6961 attggatcgc gtgcgagaag tagaattggg acatccggca ccgtggggcg aggcaaacgg
     7021 gcggctggtg tacgtggcga cgacgccggg cgacgtggcg agtcatctga ccacaaagtt
     7081 gaaacagatc taacgatgtc acggacggca caagacgtat tggagttcga caagctgcgg
     7141 gaattgctgc ggttgaggac gacgtgtgcg ccggggcggc gagctgtgaa tggactcgaa
     7201 ccagggaggg agcgcgcggc actggaatcg gcgttcgcgc tgattcgcga agcaggggaa
     7261 tggctacgcg cggggagcga gctgggattt ggcgcgctgg ccgatccgca aggctggctg
     7321 gaacggatcg agggcccggg catggcgctg gggccgggcg agttcctgga tgcggcgtcg
     7381 ttgctggaga cggcgggatg gctgcgacag caatttcgcg aggaagcggc gaagtttcct
     7441 ttgttggcgg cgcgggccgc tgcgcttggg gattttagag atgtgcacgc agcgatccgg
     7501 cggtgtgtgc tgccaaacgg cgagatcagc gacgatgcgt cggcgacgct gcggcgaatc
     7561 cgggcgagca tcacggagac gcgcgatacg attcaaaaga atctgaaaca gattttgcgc
     7621 gcgcggaatg cggaagcggg ggaagactac gttacgctgc gaaacgatcg tttcgtgatt
     7681 cccgtgcggg ccgagcatcg gcgaagcctt cccggcgtgg tccacggtgc gagcgcgacg
     7741 gggcaaacgg ttttcatgga gccgttcgaa accgtcgagt cgaacaatca gttggtacaa
     7801 cttgccgagg acgaagccgc ggaaatcctg cggattctgc gcgagcttac ggagaaactg
     7861 caaatgatcc ggggacccct gctggcggca gccggaacga ttgcggaact cgacagcgtg
     7921 tttgcgcggg cgcgattcgc gcgggatttt gacgccgcga tgccggaatt ctctggcaca
     7981 aatgaattgc gtctggaagg cgcgcgccat ccggtgctgg aagacaaact ccgcaaagaa
     8041 aatcgcgcca ttgttccgat gacgttgacc ctcggaggag aagaacgcgt gctggtgatc
     8101 agcggaccaa acaccggcgg caagaccgtg gtgctgaaaa ccacgggaat ggcggcgctg
     8161 gcggcgcagt ccggaattcc ggtggccgcg cagcgggcgg ttctgccgct gttcgaccgt
     8221 gtgctggtgg acatcggcga cgagcaatcg atcgctgcgg atttgtccac tttctcgtcg
     8281 cacatgttga acttgaaaac catgctggag gcagcgacgc cgaattcgct cgtgctggtg
     8341 gatgaaatgg gcacgggaac tgcgccggag gaaggcgccg ctctggcagt ggcgctgctg
     8401 gacgaatttc gcgcgaagca ctgcatcgtg ctggcgacca cgcatcacga ccggctgaag
     8461 acgtacgcat cgactacacc gggcgtggtg aacgccgcgg tggaattcga cgacgtaaat
     8521 ctgcggccca cataccgctt gatggtgggc gtgccgggcg gctcgagcgg aattgcgatc
     8581 gcgaa
//
LOCUS       DASYVO010000512         7489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_70393, whole genome shotgun sequence.
ACCESSION   DASYVO010000512 DASYVO010000000
VERSION     DASYVO010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7489
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70393"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1175)
                     /gene="gap"
                     /locus_tag="VGR03_17755"
     CDS             complement(171..1175)
                     /gene="gap"
                     /locus_tag="VGR03_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV1996184.1"
                     /translation="MPIKVGINGFGRIGRNIVRTALDDKDIQFVAVNDITDAKTLAHL
                     LKYDSVLGNLPHNVTHTDDTISVEGKSFKVFKTKDPAEIDWASVGADIVVESTGLFTK
                     GVDAKKHLRAPVKKVIISAPADGPDATFVLGVNDKTYNPAAHHVISNASCTTNCLAPV
                     AKVLHDTFGINCGTMTTIHSYTNDQKLLDLPHKDLRRARAAALNMIPSSTGAAKALHL
                     VIPELKGKLDGYAMRVPTPNVSVVDLTVFVDKPATKETVNAALKTAATGQMKGILGYT
                     EEQLVSADFKGNSNSSIVDAEYTKVVGDRCVKVLAWYDNEWGYSCRCRDLIKLLASKF
                     "
     gene            1308..2918
                     /locus_tag="VGR03_17760"
     CDS             1308..2918
                     /locus_tag="VGR03_17760"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HEV1996185.1"
                     /translation="MRGWSPASVLRWLFDSEHGASGILLPRWIFLRALGLIYYSAFFS
                     LVFQIHGLIGPHGILPAGEYLQALTERFGHVGYWYAPTLLWLSSSSHMLTGICWAGMI
                     ASLLLVLNFWPRGMLVVCFVCFLSFVSAAQDFSAYQSDGMLLEAGFIALFFAPAGFRP
                     GWGEESRPSRASLFLLVWECFRIYFESGVAKIMGGDPQWRNFTALDEYYQNGPLPTWI
                     GWYMQHFPHWFHAATAFGTLALELGLVWMMLLPRRMRILSSLIITPWQMGIILSANYT
                     FLNYLVLALGILLLDDRFLLPYLPRFLKESYLATKEAKSLAAPTSENKWRQRLRVQLC
                     ALKLAVAAVMLTWIFYATFAQMVWMVKRWPLPTTPVSALEPFRIANRYGLFAVMTRGR
                     YEIEFQGSVDGQTWLVYPFRFKPQDPSKAPGIYAPYQPRFDWNLWFASLSSWRQEPII
                     VRTEQSLLRGDADVQLLFSGNPFPHAPPRQVRAVIWQYWFTTATEKRSQGAWWRRQQL
                     GLYAPTLELESDGRIVVLQWPPASEPRE"
     gene            complement(3476..4405)
                     /locus_tag="VGR03_17765"
     CDS             complement(3476..4405)
                     /locus_tag="VGR03_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996186.1"
                     /translation="MSNWLALSLTLLLFADADVPRVNSLRPPASQPPSFPSDEEIPLF
                     PNTGASSDNGQSQSQSQPKTASTAKNTTLQETSKLNLIRYVSGEFAKATKALPAGKEG
                     VHVNVGKPLETDLLDRAVATHGPAVNTGDSVQITRLEFRDHQIIVDVNGGGRGKRRWR
                     DHLQIGLGGSGYPPMQTASTQDQGPPGMQPGMGSTIFLEFNKPLPDITPDELKQLLSP
                     FLNFSKERSAAVQWFDTLPPEMKKAIQDRKPMVGMDREELVAAIGKPEHKVRERDSDG
                     NDIEDWIYGQPPSKTVFVRFQGDRVTSIKQFPQ"
     gene            complement(4565..5575)
                     /locus_tag="VGR03_17770"
     CDS             complement(4565..5575)
                     /locus_tag="VGR03_17770"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996187.1"
                     /translation="MIIESKAPTRVDFAGGTLDIWPLYLFHPGAVTVNAAISLYASCV
                     IETHGPGDNRIKLVSRDIKREESFASFAALVKAKRYRLPLLAEIVKFFQPQGGFTLTT
                     DSEAPAGAGIGGSSAMAVAICAALDRFTGAGKSKVDWIHISRDAEAIVINVPTGTQDH
                     YPPAFGGAAAIELPPGGEHRVELRVNLDELERRVMVCYTGKPRQHGINNWEVYKAHIG
                     GKRAVQNSLERISGVAQEMRLAFEKPDWPEAGRLMREEWSFRKRNLPTISTKTIDRVI
                     DSTRRNGALAGKVCGAGGGGCVVLLVEPDARERVETAIHDAGAEVLPMTIDRQGVQVV
                     SR"
     gene            complement(5735..6367)
                     /locus_tag="VGR03_17775"
     CDS             complement(5735..6367)
                     /locus_tag="VGR03_17775"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003296.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV1996188.1"
                     /translation="MDIVKGNMGWIEVIVGPMFSGKSEELIRRLHRAEIARQRVQIFK
                     PAIDERYAANEIVSHSGLGIPSDAVFKAAEIMEKLSPRTEVIGIDEAQFLGDEVVDVC
                     TKLANLGKRVIVAGLDTDYRGRPFEPMPRLLAIAEEITKLLAICVRCGNPAVHTQRLV
                     ESEELIVVGAMGAYEARCRRCFEPNPALAQEKKDSPQLIARNRAASAGGS"
     gene            complement(6455..7108)
                     /locus_tag="VGR03_17780"
                     /pseudo
     CDS             complement(6455..7108)
                     /locus_tag="VGR03_17780"
                     /inference="COORDINATES: protein motif:HMM:NF014041.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF84 family protein"
BASE COUNT         1487 a         2054 c         2311 g         1637 t
ORIGIN      
        1 aagtccacgc gaatgaagac gcgctttcca cgaagatcga gatcgttgat ggtgagcttg
       61 ttcatggttg aagtccgtgc cgtgcgatca ggagtcgaat gccaagattt ggtggttgag
      121 atccttaggg cccgacaaga gtcggggcct aaggatctca agctctaatc ctagaacttg
      181 gaggccagga gcttgatgag gtctcggcaa cggcaggagt agccccactc attgtcgtac
      241 caggccagaa ctttcacaca gcggtcgccg acgaccttgg tgtattcggc atcgacgatg
      301 gaagagttgg aattgccttt gaagtcagcg gagacgagtt gctcttcggt gtagccgagg
      361 atgcctttca tctggccggt ggccgcggtt ttcagcgccg cgttgacggt ctctttggtg
      421 gcgggcttat cgacgaaaac ggtcaggtcg accacgctga cgttgggcgt gggcacgcgc
      481 atggcgtagc cgtcgagctt gcctttcagc tcaggaatga cgaggtgcaa tgcttttgcg
      541 gcgccggtgg aactgggaat catgttgagc gccgcggcgc gggctcggcg gagatccttg
      601 tgcgggaggt cgagtagctt ctgatcgttt gtgtaagagt ggatggtggt catggtgccg
      661 caattgatcc cgaaggtgtc gtggagaacc ttggcgacgg gcgcgaggca gttggtggtg
      721 caggaagcgt tggaaatgac gtggtgcgcc gcgggattgt aagtcttgtc gttcacgccg
      781 aggacgaacg tcgcatccgg gccatcggcg ggcgcggaga tgatgacttt tttcacgggc
      841 gcgcgaagat gcttcttggc gtcaacgcct ttggtgaaga ggccggttga ttcgaccacg
      901 atgtccgcac cgacagaagc ccaatcgatc tcggcgggat ccttggtctt gaaaactttg
      961 aagctcttcc cttccacaga gatggtgtca tcggtatgcg tgacgttgtg aggcagattg
     1021 ccaaggacgg agtcgtactt caagaggtgc gcgagggtct tcgcgtcggt gatgtcattg
     1081 acagcgacga attgaatgtc cttgtcgtcg agcgcggtac gcacgatgtt gcgaccgatg
     1141 cggccgaagc cgttgatgcc aaccttgata ggcattactt cctccgatgg agtgtcagaa
     1201 attgcggctg tacaggagcg catgacgccg cgggtgaaca gaaaatgcta tgggttgtgc
     1261 ggacaaaagt caacgcgcgt attattaggc cgctataatg gccgtcgatg aggggctggt
     1321 cacctgcgtc cgttttgcgc tggctgttcg attcggaaca tggcgcttcc ggtatactgc
     1381 ttccccgctg gattttcctt cgcgctcttg gcctcatcta ttactcggcg ttcttctcac
     1441 tggtctttca aattcacgga ttgattgggc ctcatggaat actccccgcc ggcgaatacc
     1501 tgcaggcgtt aaccgagagg ttcggccatg tgggctactg gtatgcgccg acgctgctgt
     1561 ggctatcgag cagctcccac atgctcacgg gaatttgctg ggcggggatg atcgcctcgc
     1621 tgctgctggt cttgaatttc tggccgcggg gcatgctggt agtttgtttc gtgtgcttcc
     1681 tttcgttcgt cagcgcggcg caggattttt ctgcttatca atccgatgga atgttgctgg
     1741 aggcgggatt cattgcgctg ttcttcgcgc cggcaggatt tcgaccgggg tggggtgaag
     1801 aaagcaggcc atcgcgcgcg agcttgttcc tactggtgtg ggaatgtttc cggatctatt
     1861 tcgaatcggg cgtggcgaag atcatgggtg gcgacccgca gtggcggaac ttcacagcac
     1921 tagacgagta ttaccagaac ggcccgctgc cgacttggat tggctggtac atgcagcact
     1981 tcccacactg gtttcatgcg gcgacggcgt ttggaacttt ggcactggaa ctagggctgg
     2041 tctggatgat gttgctgccg cggcgaatgc gaattcttag ctctctgatt atcacgccat
     2101 ggcagatggg gatcattctc tcggcgaatt acacgttctt gaattacctc gtcctggccc
     2161 tggggattct gctgctggat gaccggttcc tgctgcccta cttaccgcga ttcttgaaag
     2221 agagttacct cgctaccaaa gaggccaaat cgcttgccgc gccgacttcc gaaaacaaat
     2281 ggcgacagag actccgggtg caactctgcg cgttgaagct cgctgtagcc gccgtgatgc
     2341 tcacgtggat tttctatgcg acattcgcgc aaatggtgtg gatggtcaag cggtggccgc
     2401 tgccaacgac acccgtctcg gcgctggagc catttcgcat cgccaaccgt tatgggctgt
     2461 tcgcggtgat gacgcgcggc cgatacgaaa tcgagttcca gggctcggtc gatggacaaa
     2521 cctggctcgt ctatcccttc cgcttcaagc cgcaagatcc aagcaaggcg ccgggaatct
     2581 acgcgccgta ccaaccgcga ttcgattgga atttgtggtt tgcgtcgctg agctcgtgga
     2641 ggcaggagcc gatcatcgtg cgcacggagc agagtttgct tcgcggcgat gcggatgtgc
     2701 agttgctttt ttcgggaaat ccgttcccgc atgctccgcc gcggcaggtg cgcgccgtga
     2761 tctggcaata ttggttcacg acggcaaccg aaaagcgttc gcaaggcgcg tggtggagac
     2821 ggcagcagct cggcctctac gcgccaacgc tggaactcga atccgacgga aggattgtcg
     2881 tgcttcagtg gccgcccgcc tccgagccga gagaatagcc ccgcctctac ggcggccgca
     2941 agcaaagcac aaaagtaccc ccgcacaccg gagattccag atagttccta aaatgataag
     3001 tggtttgatt tagaattggc ccgtgtctga cgaaaacgaa atctacaaaa tcagggaaga
     3061 ggaaatggaa cgggggcgcc gacccaagca ttccagcgaa aaggagaaat ttcggcgttt
     3121 gaaatcaatg atgttggatg cgttgcacag aggaaataga ggtcttttcc agcaagtcct
     3181 tatcgacctc ggacaaaaac ccgggtcttc cgagttcgag tcatcgatga agatttacga
     3241 tgagtaccag cgcgagaagc gccggtgagt ctgcgctcgg cagccgccaa ccgtttctct
     3301 tgctcgtctt cagagaaggc gttcaatcga tgcaaagcaa gctccgccaa tttcccagct
     3361 accttgttgg cgttcggctt tttcttcctt ggcaattggc acctctcaac gggaatcggc
     3421 atccagtgct cgtcaatcag agaataacac cgttgatctg ttcctcgcgt gaggattact
     3481 gagggaattg tttaatcgaa gtcacgcggt cgccctggaa gcggacgaag acagtcttcg
     3541 agggcggctg gccgtaaatc cagtcttcga tatcgttgcc atcggaatcg cgctcgcgca
     3601 ctttgtgttc gggcttcccg atagcggcaa ccagttcctc acggtccatg cctaccatgg
     3661 gcttgcggtc ctgaatggcc ttcttcattt ccgggggcag cgtatcgaac cactgcacgg
     3721 cagcggaacg ctctttcgaa aaattcaaga aaggcgagag gagctgcttc aattcgtccg
     3781 gcgtgatgtc cggcagtggc ttgttgaatt ccaggaaaat cgtgctgccc atgccaggtt
     3841 gcatacccgg cggaccctga tcctgcgtcg aggccgtctg catcggggga taaccgctgc
     3901 cccccaggcc gatttggaga tgatcgcgcc agcgccgctt gccgcgccca ccaccgttca
     3961 catcgacgat gatctgatgg tcgcggaact ccagcctggt gatctgcacg ctatcgccag
     4021 tattgacggc cgggccgtgc gtggcgacgg cgcgatcgag cagatcggtc tccagcggct
     4081 tgcccacgtt gacgtggaca ccttctttgc ccgcaggcag cgccttggtg gctttggcaa
     4141 attcaccgga gacgtagcgg atcagattca actttgaagt ttcctggagt gtggtgttct
     4201 tcgcagtgga tgcggtcttc ggctgggatt ggctctggga ctgtccgttg tccgaggatg
     4261 caccggtgtt cgggaagaga ggaatctctt cgtccgaagg aaagctgggg ggctggctcg
     4321 cgggcggacg aaggctgttg acccgcggaa cgtccgcgtc ggcaaagagc aaaagcgtta
     4381 gcgataaggc gagccagttt gacatatctt tggtgggcct cgacctaggc ggggcccact
     4441 cactctattc tacgatgcca cgaagccgat gaacaggtgc ctcgctcggg agcggacacc
     4501 tctctcttac ctgtcctcga ttggggttga actgtacaag cggacgggga ttcgcgccat
     4561 tcccttagcg agacacgact tggacgcctt ggcggtcgat ggtcataggc agaacctcgg
     4621 cgccagcgtc atgaatggcc gtctccacgc gttctcgggc gtctggttcg accaagagga
     4681 ccacgcagcc gcctccaccc gctccgcaga ctttgccggc cagcgcgcca ttgcgacggg
     4741 tgctgtcgat aacgcggtcg atggtttttg tcgagatcgt aggcaagttt cgcttgcgga
     4801 aggaccactc ctcgcgcatc aggcggccgg cttcaggcca gtctggtttt tcgaacgcca
     4861 ggcgcatttc ttgagcgacg ccggaaattc gctccaggct gttctgcacg gcgcgtttgc
     4921 cgccgatgtg cgccttgtaa acttcccagt tgttgatgcc atgctggcgc ggcttgccgg
     4981 tgtagcagac catcacgcgg cgctcgagct cgtccaggtt cacacgcagt tcgacgcgat
     5041 gttcaccgcc gggcgggagt tctattgccg cggcgccgcc gaaggcgggc gggtaatgat
     5101 cctgcgttcc ggtaggaaca ttgatgacga tggcttccgc gtcgcggctg atgtggatcc
     5161 aatcgacctt gctcttccct gctccggtga agcggtccag cgcggcgcaa atggccacgg
     5221 ccatggcgct ggagccgccg atgcctgcgc ccgcgggagc ttccgaatcg gtagtgaggg
     5281 taaaaccgcc ctgcggttgg aagaacttga cgatttcagc aaggagcggg aggcgatagc
     5341 gcttggcttt gacgagggca gcgaaggaag caaaactttc ttcgcgcttg atgtcgcggg
     5401 aaaccagctt gatgcggttg tcgcccgggc catgcgtttc gatgacgcac gaggcgtaaa
     5461 gcgaaatcgc ggcgttcacg gtaaccgcgc ccggatgaaa cagatagagt ggccagatgt
     5521 cgagcgttcc cccagcgaag tccacgcgcg tcggtgcttt ggattcgatg atcatgtgtg
     5581 cgagctagct cgattcagag ggattttcag ccgagccagt ttgacggatt cacgcggcca
     5641 cctcaagcga tgaccgcgat gagtgtccac ggaaaggcaa tgacatccgc accggggaaa
     5701 gagcaaagaa ctgccctcga taaagaagag cgagtcagct gccgccggcg gaggcggcgc
     5761 ggttgcgggc gatgagctgc gggctgtctt tcttttcctg ggcgagagcg ggattaggct
     5821 caaagcaacg acggcagcgg gcttcgtagg cgcccattgc gccaacaacg atcagctctt
     5881 cgctttcgac gaggcgctgg gtgtgaaccg cgggattgcc gcaacgaacg cagatcgcca
     5941 gcagcttggt aatttcttcg gcgatggcga gaaggcgcgg cattggctcg aaggggcggc
     6001 cgcgataatc ggtatccaga ccggcgacga tcacgcgttt gccgagattt gcaagcttgg
     6061 tgcaaacatc cactacctca tcgccaagga attgcgcttc gtcgatgccg atgacttccg
     6121 tgcgcggcga cagtttttcc atgatttcgg cggctttgaa gacggcatcc gaaggaatgc
     6181 cgaggcccga gtgcgacaca atttcgttgg cggcgtagcg ttcgtcgatg gccggcttga
     6241 agatctgcac gcgctggcgc gcgatctccg cgcgatgcag ccggcggatc aattcttcgc
     6301 tcttgccgga aaacatcggg ccgacgatga cttcgatcca gcccatattg cctttgacga
     6361 tgtccacggt cgctccctca aagcagttag cagtaatcag ttttcagtaa agaaattcaa
     6421 gctcgaatga tatgagcgta tatctgtcat gactctaccg aaccaagccc gaggcgccga
     6481 cggccgcagc gcgatacatc ttggcattat agaacggtgc gaaggccgca agcacggcaa
     6541 ctcgaaaggc ctcctgccgc gtgatgaaat tgttcgagag cacgccccag gcgccttgcg
     6601 cgtcgcggat cccgacggcg ccggcaaacc gatcaatcgc tacggcaagc tccacgccat
     6661 tttcgagaac ttcgtgagcc agcgcttccg gcagctcgat gcttcccgag cgaccgtagt
     6721 ggcctcgcgc gccatcggag acgtaggccc agctttccag gaagacgcgg cggtgcccgt
     6781 tttgcctcaa tcccgaatga cgcagcatct cgtccgctga ggcaccttcg tggacgacat
     6841 cgagcccgcc ttccaggccg acgaagtatt accaggccgc cccgccttgg cgcgccatct
     6901 ccaccagcgc ttccgctcgt tggcgggcgc cgcgcatcaa ttcatcccgc gacgctggcg
     6961 tgtggctgac gccgctctcg acttcaacgc cgacgacttc gaagtgagcc tccggagcaa
     7021 gaacgcctga aaacgcctgg agcgcttccg tgacggcgtt gagctttggg ccacgcttgc
     7081 taccgacggc gatagtgatt ttttccattt ggaaaaatgg cgacggcccg attctaacgg
     7141 tacttgggca aaaatttcac cgggaattcg cgaggctgag gaaaactccg atggagactt
     7201 tcaaagaact catccttgcc ggatgaataa tggagcggcc tatcggaatc gaaccgacac
     7261 ctgaaccttg gcaagtctgc agagaaagga ttatcaagaa cttacaaggc attttcagga
     7321 gcatccagta gcgtttagag cggtcagaag ggcttaatgg tccttcagtt ggtcccacgc
     7381 ggaagtcggg ttggcaccct ttattgttga accgcgagcg ggttcagaaa atactccacg
     7441 ataggtcggg cacgaagaga ttcgaaccgt cgacctccag gttcctccc
//
LOCUS       DASYVO010000513         6345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_83720, whole genome shotgun sequence.
ACCESSION   DASYVO010000513 DASYVO010000000
VERSION     DASYVO010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83720"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VGR03_17785"
     CDS             <1..480
                     /locus_tag="VGR03_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996189.1"
                     /translation="GVGDANAGKSPQKAARKFLLGLAIPPGWSNQEWELLALVVRYHR
                     GAEPRAKDGPFSKLPAEQQKSVRALAGVLRLARALRKCGVATGAGLRAEKSMDAIVLR
                     VPGLLDDIETAARLAAGKHLLEEVLRVPLIVKPAAKPEKVVALTPRQVPEFSVQASD"
     gene            complement(495..995)
                     /gene="sixA"
                     /locus_tag="VGR03_17790"
     CDS             complement(495..995)
                     /gene="sixA"
                     /locus_tag="VGR03_17790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEV1996190.1"
                     /translation="MQLYIVRHGIAIDREDPKCPPDPERYLTEGGVEKTKQVAKGVAA
                     LGVTADLFLTSPYVRAAQTAEIFASALDYAKQKIRRTDLLLPGAEPSLLFRELAKDKQ
                     ASTVFLFGHVPQLDDLIATALGSKKHLTGLKKAGVALIELKRLSPPIGELVWLATPKL
                     LRRAGK"
     gene            complement(1059..2600)
                     /locus_tag="VGR03_17795"
     CDS             complement(1059..2600)
                     /locus_tag="VGR03_17795"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEV1996191.1"
                     /translation="MPEPVHIAAIDAGSNAVRLSVARAYSALDIEPVHMERYPLRLGE
                     SVFVRPRFSEEISKKAAKAFRHFREVMDEFGVTRYRAVATSATREARNRKAFVRRIKQ
                     KSGITLEVISAAEESRLGREAVLAALGPEAPPRCIADLGGGSLEISILMDHTVEQGAQ
                     LPAGTVRLMTTLDLPGVIRPAEAEQVRRYVRALLESKLTPRPNLGESIAVALGGNAET
                     LANVAPGPRQHGLQTMDLSLLRARLPDILRRDARERMKVYAVRRDRADVMGVAAITFL
                     TLGRYLNLRTFAVPGVGVREGLLQEIAREAFSRKEPHRYNAAARQLLVGTRSFARRLG
                     YEQQHAEHVRELSIQLFDQLQPVHHMVAHSRVLLEAGALLHDTGHMISHRGHHKHGEY
                     LALNGDIPGLEGRDRAVAAALVRYHNRKSEPAGHHTAYSSLNNADKRITRRLAAILRI
                     AESLDHSHRQRVLKLRASFQRGAVGLQVHARGDATEDLRDANRSAELFEKEFHVRLYF
                     RQSPV"
     gene            complement(2609..3454)
                     /gene="tmk"
                     /locus_tag="VGR03_17800"
     CDS             complement(2609..3454)
                     /gene="tmk"
                     /locus_tag="VGR03_17800"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV1996192.1"
                     /translation="MPDQEKEKTAAAVPSTETPAAAPPAAAAKTAVVTEEASSQYFFG
                     EPLVGFDPSEITGSLVVIEGMDGSGRSTQIALLQEWLESEGFAVQTSGLRRSNLVGRD
                     IDELLAKNAATRLTLALMYATDFFDQVEHRILPALRAGTIVLADRFIFTLIARGVVRG
                     INRDYMSGLYAMALRPHLTFWLNVRPETAFGREFKKAQAISYWEAGRDMSLSHDLYWS
                     FIRYQTMIKREFEVMAKKHSFLELDGEASVPTVNKQLRQRIGEQLGIRATKYTPSAAL
                     THLWR"
     gene            complement(3457..4221)
                     /gene="tmk"
                     /locus_tag="VGR03_17805"
     CDS             complement(3457..4221)
                     /gene="tmk"
                     /locus_tag="VGR03_17805"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV1996193.1"
                     /translation="MTETQDVVQKAPRTEPAKSTLPQPGRPVPSRAYPGALIVVEGTD
                     GSGKSTQLYLLKRWLEIGGYRLHFTEWNSSPLVKSATRRGKQRRLLTPTTFSLLHAAD
                     FADRCERQIMPLLQGDYLVLADRYIYTAFARDAARGCPARWLRNLYRFAPIPDITFYF
                     RAPLDVAVHRILSGRPKLKYYEAGMDLGLSYDRAESFRLFQARILEEYDKMVDTDNFV
                     VLDGTLPVNKLQKQMREIVASKIDLKRFAPRVQESE"
     gene            complement(4485..5573)
                     /locus_tag="VGR03_17810"
     CDS             complement(4485..5573)
                     /locus_tag="VGR03_17810"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV1996194.1"
                     /translation="MASRKQLTWTELRVGLFVLVGLFVLAAGIFYVTGQGSFMGPKYR
                     LKTYLPEVAGLTSGAPVRVDGVEVGNVESIRLLPRTPGKAPEKNKNIEVVMRVDKRYQ
                     SDILTDSTASLVTEGLLGNRYVNITRGFTGTSIGDAGVIPGAEEKAMKEVVERSAEVL
                     GNLNALSEDVRQLIQGVKEGKGSLGKLLTDEQAYNHLNSLLAKGDVMLANIQAGQGTL
                     GKIVTSDEMYTKVDKGLDNVNVILADVRAQKGTIGKLMYDPTLYDQAKQAITNGNAMI
                     GDVRAGKGTLGKLATDDTLYNKLRDTSSNLATASAKLNDNTTTAGKLFSDPKLYDNLA
                     GLTGDMRLLIGDFRQNPKKFLHIKITLF"
     gene            complement(5618..>6345)
                     /locus_tag="VGR03_17815"
     CDS             complement(5618..>6345)
                     /locus_tag="VGR03_17815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1996195.1"
                     /translation="QERETLILLGETGTGKTLTLKMAAGLLTPNSGKVTVLGNAVSEM
                     KESELLAFRQKVGFVFQEGALFDSMSVADNVAYRLREERVREEEIESRVLEVLRFVEL
                     EDTLELLPSELSGGMKRRVSIARALINHPPIVLYDSPTAGLDPVTSQTIITLILRLRD
                     VYGVTALLATHRLQDGFALANFHFDPDTKRVVPNSLDGNAARAANSARTRFLVYRDGS
                     IYFEGEPEEIANSEDPYLKRFLV"
BASE COUNT         1211 a         2108 c         1668 g         1358 t
ORIGIN      
        1 ggggttggcg acgccaatgc cggcaagtca cctcaaaaag ccgcgcgtaa attccttctt
       61 ggtctcgcca ttcctcccgg ttggagcaac caagagtggg agctgctcgc tcttgtggtc
      121 cgctaccatc gcggcgccga accccgcgcg aaggacggtc ctttttccaa gcttccggcg
      181 gagcagcaga aaagcgtccg cgccctggct ggcgttttgc gcctggcgcg cgcgcttcgc
      241 aaatgcggtg tcgcgactgg cgcggggctt cgcgccgaaa aatccatgga cgccatcgtt
      301 ctgcgtgttc ccggcctgtt ggatgatatc gaaaccgccg cgcgccttgc cgcaggcaag
      361 catctgctgg aagaggttct gcgcgtgccc ttgatcgtga agcccgccgc gaagccggaa
      421 aaggtcgttg ccctgacgcc ccgccaagtt ccggaattct ctgtccaggc ttccgattga
      481 agcaactcgt tgttctactt ccccgctcgc cgcaacaatt tcggggttgc cagccacacg
      541 agttcgccaa tcggcggaga gaggcgcttg agttcgatca aagccactcc cgcctttttc
      601 aaccctgtca gatgcttctt cgagccgagc gccgtagcga tgaggtcatc aagctgcggc
      661 acatgcccaa aaagaaagac cgtggacgcc tgcttgtcct tcgcaagctc ccgaaaaagc
      721 agcgaaggct ccgctcccgg caacagcaag tccgttcgcc gaattttctg cttggcgtaa
      781 tccagcgcac tcgcgaaaat ttccgcggtc tgtgccgcgc gcacgtaagg actcgtgagg
      841 aacagatccg ccgtcactcc cagcgccgcc acgcctttcg ccacctgttt tgtcttttcg
      901 acgcctccct ctgtcaaata ccgttccggg tcgggagggc acttggggtc ctcgcggtcg
      961 atggcgatcc cgtgccgaac aatgtagagc tgcatcctct tctccttcga atcgatcgag
     1021 cgccggccag caccagtgca gcgcgtcact ccgatccctt acaccggcga ctgtcggaaa
     1081 tacagcctca catggaattc tttttcaaat agctccgcgc tgcgattggc gtcccgcaag
     1141 tcttccgtcg catcgccacg cgcatgcacc tgcaaaccca cggccccgcg ttgaaaggaa
     1201 gcgcgcagtt tcagcacccg ctgccggtgt gaatgatcta gagactcggc gattcgcaaa
     1261 atcgccgcca gtcgccttgt gatccgttta tccgcgttgt tcagcgaaga ataggcggtg
     1321 tgatgccccg caggctccga ctttcgattg tgatagcgca ccagcgccgc cgccaccgcg
     1381 cggtcgcggc cctccagacc cgggatatcc ccgttcagcg ccaggtactc tccatgcttg
     1441 tgatgcccgc ggtggctgat catgtgtccg gtatcgtgca ggagagcgcc cgcctccagc
     1501 agcacccgcg aatgcgccac catatgatgt accggctgca actgatcgaa cagctggata
     1561 ctcagttccc gcacgtgttc cgcgtgctgc tgttcgtatc caagccgccg cgcaaaactc
     1621 cgcgtgccca caagcaactg ccgcgctgcg gcgttgtacc ggtgtggctc tttgcgggaa
     1681 aacgcttccc gcgcaatctc ttgcagtagc ccttcgcgta cgccgacgcc ggggacagca
     1741 aaagtgcgca gattcagata gcgccccagt gtgagaaatg tgatcgccgc gacgcccatc
     1801 acatccgcgc ggtcgcgccg caccgcataa accttcatgc gctcgcgcgc gtctcgtctg
     1861 agaatgtccg gcaggcgcgc ccgcaggaga gacaagtcca tcgtctgtag cccgtgctgc
     1921 cgcggccccg gagctacatt cgccagcgtt tccgcattcc cccccagcgc caccgcaatg
     1981 ctctcgccca gattcggccg cggcgtcaat ttcgactcca gcaacgctcg cacgtaccgc
     2041 cgcacctgct ccgcttccgc cggacgaatc accccgggaa ggtccagcgt ggtcatcaac
     2101 cgcaccgtgc ccgccggcaa ttgcgcgcct tgctccacgg tgtggtccat caggatgctg
     2161 atctccagac tgccaccacc cagatccgcg atgcatcggg gtggcgcctc cggtcccagc
     2221 gccgccaata ctgcttccct gccgaggcgc gattcctccg ccgcgctgat cacttccagc
     2281 gtgattcccg atttctgttt gatgcgccgc acaaacgctt tgcggtttcg cgcttcacgc
     2341 gtggcgctcg tggctaccgc gcgatatcgc gtgacgccga attcatccat cacttcgcgg
     2401 aaatgccgaa aggccttcgc ggcttttttg gaaatctctt cgctaaagcg gggccgtacg
     2461 aaaacgctct ctcccagacg cagggggtag cgttccatgt gcacgggttc gatatccagt
     2521 gcggaataag cgcgcgccac ggacaagcgc accgcgttgg aaccggcatc gatggccgcg
     2581 atatgaaccg gctcgggcat ttcgggatct accgccagag gtgcgttagc gcggcggaag
     2641 gtgtgtactt cgtcgcacga attcccaatt gctcgccaat gcgctggcgc agttgcttgt
     2701 tcaccgttgg aacgctggcc tcgccgtcca gttcaaggaa gctatgcttc ttggccatga
     2761 cttcgaactc gcgcttgatc atcgtctgat agcggatgaa agaccagtag agatcgtgcg
     2821 aaagcgacat gtcccgtccg gcttcccaat aactgatcgc ttgcgctttc ttgaactcgc
     2881 gcccgaaggc cgtctccggc cgcacgttca gccagaacgt caggtgcggc cgcaaggcca
     2941 tagcatagag cccgctcatg taatcgcgat tgatgccgcg caccacgccg cgcgcgatga
     3001 gtgtgaatat gaatcgatcc gccagcacga tggtaccggc tcgcaacgcc ggcagaatgc
     3061 ggtgttctac ttgatcgaaa aagtccgtcg cgtacatcag cgccagcgtc agccgcgtcg
     3121 cggcgttctt ggccaaaagt tcatcgatgt cgcgccccac cagattcgaa cggcgcaagc
     3181 cgctcgtctg caccgcgaac ccttccgatt ccagccattc ttgcagtaac gcaatctgcg
     3241 tcgaacgccc cgacccatcc ataccttcaa tgacgaccag gctgccggta atttcgctcg
     3301 gatcgaatcc gactagtggc tcgccaaaaa aatattgcga cgaagcttct tcggttacca
     3361 ccgccgtttt cgccgcagcc gccgggggcg cggccgccgg cgtttccgtc gagggaaccg
     3421 ccgctgccgt tttctctttt tcctggtccg gcatagtcac tccgattcct gaacgcgtgg
     3481 agcgaagcgc ttgagatcga tttttgaagc cacgatttcg cgcatctgtt tttgcagctt
     3541 gttcacgggc agcgtgccat ccagcaccac gaaattgtcg gtgtccacca tcttgtcata
     3601 ctcctcaagt atccgcgcct ggaacaaccg gaaactctcc gcgcggtcgt acgacaatcc
     3661 cagatccatg cccgcttcgt aatatttcaa tttcggacgc ccgctcagga tgcggtgcac
     3721 cgccacgtcc agcggcgcgc ggaaataaaa cgtaatgtcc ggaatcggtg caaatcggta
     3781 taaatttcgc agccatcgcg ccgggcagcc tcgcgccgca tcccgcgcaa acgccgtgta
     3841 aatgtagcga tcggcaagca ccaggtagtc gccttgaagc agcggcatga tctgccgctc
     3901 gcagcggtct gcgaaatccg cggcgtgcaa cagcgaaaac gtcgtcggcg tcagcaaccg
     3961 ccgctgcttc ccgcgacgcg tcgcggattt caccagcggc gaggaattcc attccgtgaa
     4021 atgcagccgg tatccgccga tttccagcca ccgcttcagc aaataaagtt gcgtgctctt
     4081 gcccgacccg tccgttcctt ccaccacaat caacgcgccc ggatacgccc ggctcggaac
     4141 cggccggcct ggctgcggaa gcgtggattt cgccggctcg gtcctcggcg ccttttgtac
     4201 gacgtcttgt gtttcggtca tcttagttga gaaaagctcg tctacttggg acagtaatcc
     4261 ttctttgtta cagtggcatt acaacacacg caaccacttc cggcaaagcg cccgattaat
     4321 tcaaaatatg gctgtaaagt tccctgcgct gtcatcctga gcgaagcgaa ggatctcaac
     4381 tgatccccgc ttccgcgtaa acattcggct gcgatccttc ggcttcgagg caagacccat
     4441 agcagaccca cgatgagagt gagaaagttc ttctccacac cgcgtcagaa aagcgtgatc
     4501 ttgatgtgca agaatttctt cggattctgc cggaaatccc caatcaacag ccgcatatcc
     4561 cctgtcaaac ccgccaggtt gtcgtacaac ttggggtcgg agaagagttt ccccgccgtc
     4621 gtcgtgttgt cattcaattt cgccgacgcc gtcgccagat tcgaactcgt atcgcgcaac
     4681 ttgttgtaaa gagtatcgtc cgtggccagc ttgcccaacg tgccttttcc ggcgcgcacg
     4741 tccccgatca tcgcattgcc gttcgtgatc gcctgtttcg cctggtcgta gagcgtcggg
     4801 tcatacatca attttccgat cgtccccttc tgcgcccgca cgtcggccag aatcacattc
     4861 acgttgtcca aacctttgtc cactttcgtg tacatctcgt ccgaggtcac gattttcccc
     4921 agcgtgcctt ggcccgcctg aatgttcgcc agcatcacgt cgccctttgc cagcaggctg
     4981 ttcaagtggt tgtatgcctg ctcgtccgtc aacaattttc ccagcgagcc cttcccttcc
     5041 ttcacgccct ggatcagttg ccgcacgtcc tccgaaagcg cattgaggtt tcccagcacc
     5101 tccgcgctcc tctccaccac ttccttcatc gctttctctt ccgcacctgg aatcacgccg
     5161 gcgtcgccga tggaagttcc cgtaaaacct cgggtgatgt tcacatagcg gttgcccaaa
     5221 agtccttcgg tgaccaggct cgcggttgaa tccgtcagaa tgtcgctctg ataccgcttg
     5281 tccacgcgca tcactacttc gatgttcttg ttcttctccg gcgcctttcc cggagtccgc
     5341 ggcaatagcc ggatcgattc cacgtttccg acttccactc cgtccacgcg caccggcgct
     5401 ccgcttgtca accccgctac ctccggcaga taggtcttca gccggtattt cggccccatg
     5461 aatgatccct gccctgtcac atagaaaatt cccgccgcca gcacgaagag ccctaccagc
     5521 acgaagaggc caacccgcaa ttccgtccac gtgagttgtt ttcgactcgc catcgctgct
     5581 tccctccctc gctaccgtga aacagactac tccaaaatca caccagaaaa cgcttcagat
     5641 aggggtcttc ggagttggcg atctcttcgg gttcgccttc gaaatagatg cttccgtccc
     5701 gatagaccag gaaccgcgtt ctggccgagt tcgcggccct cgcggcgttt ccatctagag
     5761 agttgggaac aacccgtttg gtatctgggt cgaaatgaaa atttgccagc gcaaaaccgt
     5821 cctgcaaacg atgcgtagcg agcagcgccg tcactccata cacgtcgcgc agccgcagaa
     5881 tcagtgtgat gatagtctgg gacgtcaccg ggtcgagtcc cgccgtcggt gaatcgtaca
     5941 gcacgatggg cgggtggttg atcagcgccc tggcaatcga gacgcggcgc ttcatgcccc
     6001 ccgatagttc cgaaggaagc agttccagtg tgtcctccaa ctcgacgaat ctcagaactt
     6061 ccaaaactcg agactcgatc tcttcctccc tgacgcgttc ttcgcgaagc cggtaggcca
     6121 cgttgtctgc gaccgacatc gagtcgaaga gcgcaccttc ctgaaacaca aacccgacct
     6181 tctgtcgaaa cgccagcaac tcgctctctt tcatctcgga gactgcattc ccgagcacgg
     6241 taaccttacc gctgttgggc gtcagcagtc ccgcggccat cttcaacgtc agagtttttc
     6301 ctgttccagt ttcgcccagc aggatcagcg tctcccgctc ctgca
//
LOCUS       DASYVO010000514         4316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_222471, whole genome shotgun sequence.
ACCESSION   DASYVO010000514 DASYVO010000000
VERSION     DASYVO010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222471"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..542
                     /locus_tag="VGR03_17820"
     CDS             213..542
                     /locus_tag="VGR03_17820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEV1996196.1"
                     /translation="MPLYEYKCLKCGRNTEKIENVSGPNLKKCPHCGGKVESVITAPS
                     IQFKGAGWYVNDYGGKKSTGGDGEKTDKPATETKETGNKEKESASKEASSKDAKESKE
                     KKPEKKK"
     gene            complement(627..1568)
                     /gene="galU"
                     /locus_tag="VGR03_17825"
     CDS             complement(627..1568)
                     /gene="galU"
                     /locus_tag="VGR03_17825"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV1996197.1"
                     /translation="MTDNTSATPNSAPFERSAVTPQKVRKAVLPAAGLGTRFLPATKA
                     QPKEMLTVVDKPQIQYVVEECVASGIEHIIIVTGKGKNSIEDHFDSAPALEHFLEERG
                     KKDQAEMVRKISNMVQVSYTRQKEPLGLGHAVLVARDLVGDEPFAVLLGDVLIPGPNP
                     ATKQLIKVYEATGVGAIAVEEVPKDKTNLYGIVDGEPAPQPPFGERLLRIRDLVEKPK
                     PEDAPSNLAITGRYVLPPAIFDCLARTKPGAGNEIQLTDALRILAREQGLWAYIYEGI
                     SYDAGDKLGFLRATVEIALQNPEFGAAFRSYLKTLKL"
     gene            1768..2889
                     /gene="tsaD"
                     /locus_tag="VGR03_17830"
     CDS             1768..2889
                     /gene="tsaD"
                     /locus_tag="VGR03_17830"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV1996198.1"
                     /translation="MRILGIESSCDETAAAVVADGREILSSVVASQIDIHRKYGGVVP
                     ELASREHLRQIVPVVREAIAEAGMTLADLDAIGVTQGPGLVGALLVGITYGKTLAQAL
                     GKPLVAVNHLEGHVHAVFLEAHKAGRTAKLPAVCLIVSGGHTVLYEVRRDDSNAQDAG
                     ARLRYRKIGQTRDDAAGEAYDKVAKLLGLGYPGGPILDRLAAVPNDAAAPVKFGPTKM
                     KGNALDFSFSGLKTAVLYHVREHPEYAPEIRVREEALQRGERKFEQLLPLCSPKTLAL
                     VREFQNAVVRDLVERTMQAAEELAVESVLVSGGVAANSQLRATFKERAKSAGIEIFFP
                     SRALSTDNAAMIAAAAYSRFRSGILADTTLNADPSLSLA"
     gene            3178..3987
                     /gene="pxpB"
                     /locus_tag="VGR03_17835"
     CDS             3178..3987
                     /gene="pxpB"
                     /locus_tag="VGR03_17835"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEV1996199.1"
                     /translation="MGYTFASMDILAGEGARFQFASDQSLLVYFDLAKGKGRAPDKVP
                     DEVGAGGTSSDRLLRSQITLQANEKVRKLLRLLELEPIAGVCNLHPAYCSLLVNFDGV
                     RFRHEEVEAILRRYLERLGEVKLPEPRHVEIPVCYGGEFGPDLAEVCAMRGMTAAQVI
                     ELHASVEYLVYFLGFVPGFAYLGELTKELVTPRLATPRKKIPAGSVGIAGNQTGVYPF
                     ATPGGWRLIGRTPLAMFRAEREGLSLLSIGDRVQFAPISTERFAVLEKEWA"
     gene            3978..>4316
                     /locus_tag="VGR03_17840"
     CDS             3978..>4316
                     /locus_tag="VGR03_17840"
                     /inference="COORDINATES: protein motif:HMM:NF014663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KipI antagonist"
                     /protein_id="HEV1996200.1"
                     /translation="MGVIQVLEPGLFTTVQDLGREGFGPMGVSASGAADAISLRLGNR
                     LVGNGEGAAGLEMTLLGGTFVFPEGAVLALTGSDFGATLDGKLVELWTSFEARAGQAL
                     RLGPTRSGARC"
BASE COUNT          891 a         1076 c         1460 g          889 t
ORIGIN      
        1 cgaagcgtga tgtcttcgag attgccgatc ggcgtaccga ccagatacag gcagccgccg
       61 gctgccggat tcgccgcgtc tgcctgcttg ggagtagaag tcttcgcgct catgctcacg
      121 agtctagcac gcgttccggg cgcgccactc gcgctcgcac gcaagagcaa tgctatactt
      181 ttcgttttga gcagcacatc ggaggagcgt cagtgcccct gtacgagtac aaatgcttga
      241 aatgcggccg caacacggaa aaaatcgaga acgtctccgg cccgaacctg aaaaaatgcc
      301 cgcactgcgg cggaaaagtg gagtcggtga ttaccgcgcc atcgattcaa ttcaaaggcg
      361 ccggttggta cgtgaatgac tatggtggaa aaaagtccac tggcggtgac ggtgagaaaa
      421 cggacaagcc cgccacggaa acgaaagaaa ccgggaacaa agagaaagaa tcggcgagca
      481 aggaagcatc ttccaaagac gcgaaagaga gcaaagaaaa gaaacccgag aagaaaaagt
      541 aaaccgaggg ggataacaga aaactggcca gcccgtcatt ccgagggtag cgaggaatct
      601 ctcctgatcc tcttgcctag cggtggctag agcttgagag ttttcaagta gctgcgaaac
      661 gcggcgccga attcaggatt ctgcagcgcg atttcaacgg ttgctcttaa aaagccgagc
      721 ttgtcgccag catcatagga aatgccctcg tagatgtaag cccagagccc ctgttcacgg
      781 gcgaggatgc gcagcgcatc ggtaagctga atttcatttc ctgcgccagg tttcgtgcga
      841 gccagacaat cgaagatcgc tggcggcaaa acgtaacgcc cggtaatcgc caaattcgat
      901 ggcgcgtcct ccggcttggg tttctcaacc aggtcgcgta tgcgcagcag ccgttcgccg
      961 aaaggtggct gcggagcggg ttcgccatcc acgatgccgt agagattggt tttgtccttg
     1021 gggacttctt ccaccgcgat ggcgccgacg ccggtagctt catagacctt gatgagttgt
     1081 ttggtggcgg gattcggacc ggggattaat acgtcgccga gcaaaaccgc gaaaggttcg
     1141 tcgccaacca ggtcgcgcgc gacgagcacg gcgtggccga gcccgagagg ctccttctgc
     1201 cgcgtgtagc tgacctgcac catgttgctg attttgcgga ccatttccgc ctgatctttt
     1261 tttcctctct cttccagaaa atgttccagt gcgggagccg aatcgaagtg gtcctcgatg
     1321 gaattttttc ctttgcccgt gacgatgata atgtgctcga tgccggaggc tacgcactct
     1381 tcgacgacgt attgaatctg cggcttgtcg acgacggtga gcatctcttt cggctgcgcc
     1441 ttggtggcgg gcaggaaccg cgtgccgagt ccagcggcag ggagcacggc cttgcgaact
     1501 ttttgcggtg tgaccgcgga gcgttcgaat ggcgcagagt tgggtgtggc ggaggtgttg
     1561 tcggtcatag ggtcagttgt ttgcacactc caagaggatg gacaccattc ttcgacgcgg
     1621 cttcgaatca gtcaatgttt ttgcgttcga acggtgcccg ttgggtaagt cgggcaggaa
     1681 tgaatcaggt gctgccggga gccgggcatg ggcttgtgct agcataattc agttgcgggg
     1741 taatgctcgg tagagatgga agaaaaattg agaatcctgg ggattgaatc ttcttgcgat
     1801 gagacagcgg ccgcggtggt cgctgacggg cgggagattc tgtccagcgt ggttgcttca
     1861 cagatcgaca tccaccggaa atatggcggc gtggtgccgg agctggcgtc gcgcgagcat
     1921 ttgcggcaga tcgttccggt ggtgcgcgaa gccatcgcgg aagcggggat gacgcttgcg
     1981 gatctggatg cgattggtgt gacgcaagga ccgggacttg tgggcgcgct gctggttgga
     2041 attacgtatg ggaaaacgct ggcgcaggcg ctggggaagc cgctcgtggc ggtgaatcat
     2101 cttgagggac atgttcacgc ggtttttctg gaagctcaca aggcggggcg tacggcgaag
     2161 ttgcccgcgg tgtgcttgat tgtttcgggt gggcacacgg ttttgtacga agtaaggcgg
     2221 gatgattcga acgcgcagga cgcgggcgcg cgtttgcgtt atcggaagat cggccagacg
     2281 cgggacgacg cggcgggaga agcgtacgac aaagtggcga agctgctggg gcttgggtat
     2341 ccgggcgggc cgattctcga tcggctggcg gcggttccga atgacgcggc ggcgccagtg
     2401 aaattcgggc cgacgaagat gaaagggaat gcgctggact tcagcttcag cgggctgaag
     2461 acggcggtgc tctatcatgt gcgggagcat cctgagtatg cgccggagat tcgcgtccgc
     2521 gaagaagcac tacagcgcgg cgaaagaaaa ttcgagcaat tgctaccact gtgcagtccg
     2581 aaaactctgg cgctggtgcg ggaatttcag aatgcagtgg tgcgcgacct ggtcgaacgc
     2641 acaatgcaag ccgcggaaga gctagcagtg gaaagcgtgc tggtttccgg cggcgttgcg
     2701 gcgaacagcc agttgcgcgc gacgtttaaa gaacgcgcga aatccgccgg catcgagata
     2761 ttttttccga gccgagcgct ttccacggat aacgcagcga tgatcgccgc cgcggcatat
     2821 tcccgctttc gctccggcat tcttgcggac acgaccctca acgccgatcc aagcctctcg
     2881 ctggcgtgaa ccgcgattgt gattttgttg agggaaagaa ttaacacaga ggttgcggag
     2941 tacgcagagc gcgcagagaa gagaagatcg gcttcgccgt gcaagcaccg aggcgggcat
     3001 aaaccgtaaa agcgggagcc gggctcccgc actccaaaga gtttccccta catcggaaat
     3061 tgtttcctga agtgaaggat tctaactctc agagcagacg ctttggatgc cgtggacggg
     3121 gattcgcggc ctgaagagga caggcaagaa tgcctgtccc actcgaggcg ccggagcgtg
     3181 gggtatactt tcgcgagcat ggacattttg gcgggtgaag gcgcgcgatt ccagtttgca
     3241 agcgatcagt cgctgctggt ttacttcgat ctagcgaagg gaaaaggccg ggctcccgac
     3301 aaggtccccg acgaggtcgg ggcaggcggg acgagttccg accggcttct acggagtcag
     3361 atcacactcc aagcgaatga aaaggtcagg aagctgctgc ggctgctcga gctggagccc
     3421 attgcgggtg tctgcaatct tcaccctgct tattgctcct tgttggtaaa ttttgatgga
     3481 gttcggtttc ggcacgagga agtggaagcg attctgcggc gatatcttga gcggctcgga
     3541 gaggtaaaac ttccggagcc gcggcatgtg gaaattccgg tgtgctacgg cggagagttc
     3601 ggccccgacc tggctgaggt ctgcgcgatg cgcggaatga cggcagcaca ggttatcgag
     3661 ttgcacgcgt ctgtcgagta tcttgtttat ttcttggggt tcgtgccggg atttgcgtac
     3721 ctaggtgaat tgaccaagga gctggtgacg ccgcggctgg cgacgccacg aaaaaaaatt
     3781 cccgcaggta gcgtggggat cgcggggaac caaaccggcg tgtatccgtt tgccacgccg
     3841 ggtggatgga gattgatcgg gcgaacgcct ctggcgatgt tccgagctga gcgggagggc
     3901 ttgagcctct tgtccattgg agatcgcgtg caatttgcgc cgatctccac ggagcggttt
     3961 gcggtgctgg agaaggaatg ggcgtgattc aggtgctaga gccggggctc ttcacgaccg
     4021 tgcaggattt ggggcgagaa ggttttggtc cgatgggcgt ttccgcttcg ggggcggcgg
     4081 acgcgatttc gctgcggctc ggcaatcgat tggtcggcaa tggcgagggc gcagccgggc
     4141 tggagatgac gctgctcggc gggacgtttg tgtttccgga gggcgctgtg ctggcgctga
     4201 cgggttccga tttcggggcg acgctggatg gaaagcttgt cgagttgtgg acttcgttcg
     4261 aagcgagagc cggacaggcg ctgcggctgg gcccgacacg ttcgggggcg cggtgt
//
LOCUS       DASYVO010000515        11240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_253402, whole genome shotgun sequence.
ACCESSION   DASYVO010000515 DASYVO010000000
VERSION     DASYVO010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 11240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11240
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253402"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            859..1392
                     /locus_tag="VGR03_17845"
     CDS             859..1392
                     /locus_tag="VGR03_17845"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV1996201.1"
                     /translation="MFLDVKELAVRKLRIRKTYAPGSIDFHSPEIKQIEPLEVTATAE
                     LLESQIRIEGNLETKIELVCARCLEPVVEEVSRTFDLFYAPLPKEAKPTIDRLKDDDT
                     EIGFYEGPGLFLADVLKEQVLLALPLKVICQSDCRGLCPNCGANLNHEECRCETHATD
                     ARMAPLARLKQDWLKKQ"
     gene            1455..1634
                     /gene="rpmF"
                     /locus_tag="VGR03_17850"
     CDS             1455..1634
                     /gene="rpmF"
                     /locus_tag="VGR03_17850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV1996202.1"
                     /translation="MPNPKRRHSKRRTSTRRANDFLTKPALAKCPNCHEMKQPHQVCP
                     HCGQYKGKAAQEPAS"
     gene            1677..2684
                     /gene="plsX"
                     /locus_tag="VGR03_17855"
     CDS             1677..2684
                     /gene="plsX"
                     /locus_tag="VGR03_17855"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV1996203.1"
                     /translation="MITIAVDAMGGDNAPRPEVEGSVLAARDLGVRVLLVGQPSVVRA
                     ELAKHASPNLPIEIVAASEVITMNDHPAQAFRRKKDSSVHVAARLVRETKADGMVSAG
                     NTGAVMTVARFLLGALESVDRVALAAPFPNAKGGVSVLLDVGANVDSKPEHLVQFAVM
                     GEVYYRATFGNKRPRVGLLSVGEEEIKGNELTREVYTRLKKSAVHFVGNVEGGDLFSG
                     NVDVIVCDGFVGNIALKICEGLAVSIFGLLRKSLKSSLMSQVGAMLSQGAFKGLKKTI
                     DYTEYGGAPLLGVRGVCVIGHGRSNANAVKNAIRVAAGLARARMNEKIEQELSAAAVI
                     A"
     gene            2707..3660
                     /gene="fabD"
                     /locus_tag="VGR03_17860"
     CDS             2707..3660
                     /gene="fabD"
                     /locus_tag="VGR03_17860"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV1996204.1"
                     /translation="MGKVAFVFPGQASQYPGMGKELAEKYPAAKAVFNEADKALGFSI
                     SKMCFEGTEEDLKLTANTQPAILTCSVAVYRVLAEKGLTPDFVAGHSLGEYSALVAAG
                     ALKFADAVQLVRKRGTYMQDAVPAGVGAMAAIMGLSPAVVADTCKRAAEGEICSAANL
                     NSPEQTVISGHASAVKRAVEIASQLGAKRAVILPVSAPFHSALMMPAQEKLEIDLKQT
                     EFAALRVPLVTNVDADTIETGDEAREALIRQVTMPVRWEESVRLLIDEGVNTFVEVGP
                     GRVLSGLLRQIERSVATLNVEDEKSLSATVEKIAGARSDAA"
     gene            3739..4485
                     /gene="fabG"
                     /locus_tag="VGR03_17865"
     CDS             3739..4485
                     /gene="fabG"
                     /locus_tag="VGR03_17865"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV1996205.1"
                     /translation="MFSLKDKVALVTGASQGIGRDTALALSEAGAKVVVGARNEEKLA
                     ALVVEIAAAGGEAFAVKMDVADAEQVKAGFKQVVEKFGRLDILVNNAAITRDGLALRM
                     KQDDWEAVIRTNLTGAHLCIQQALHTMMRARAGRIINIASVVAQMGNAGQANYVAAKA
                     GLIGLTKAIAMEIASRNVTVNAVAPGFIKTPMTDVLPDKVKEELNTRIPLGRMGSARD
                     VAAAIVFLASDEAGYITGHVLDVNGGMYLA"
     gene            4482..5051
                     /locus_tag="VGR03_17870"
     CDS             4482..5051
                     /locus_tag="VGR03_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996206.1"
                     /translation="MSLLRTSAVICLLLGSCAASLASEDVAKTLAQHPVWIGRGLKWE
                     HIEGDPDPKQTVAGATVLYLGRNGKFAMVSGIVVKRAAGMIISEGDGEADSIGTWAVH
                     DGTVHVSYRLVSNYKIAHKVEEPEPKIPGPVEKAEISFEHKQGDDKKPVTLLQFQGHE
                     YEIARGLPVSEMKDRLEIYKASSAAETLR"
     gene            5297..5539
                     /gene="acpP"
                     /locus_tag="VGR03_17875"
     CDS             5297..5539
                     /gene="acpP"
                     /locus_tag="VGR03_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1996207.1"
                     /translation="MASVEERVKQIIVEQLGVDEAEVTPTASFVDDLGADSLDQVELV
                     MAFEEAFGIEVPDEDAEKMTTVKDAVEYIDKHAKGK"
     gene            5578..6822
                     /gene="fabF"
                     /locus_tag="VGR03_17880"
     CDS             5578..6822
                     /gene="fabF"
                     /locus_tag="VGR03_17880"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV1996208.1"
                     /translation="MTRRVVVTGVGLVCALGIGTEETWKNLVAGKSGVGPITHFDTTG
                     FDCKIAGEIKNFDPFQWIEKKELKKMGRFIQVALAAADFAVKMSGWKPENSDLDEVGV
                     YISSGIGGFDIIEREHIKLMQGGPGRISPFFIPSAIVNLASGHISIRYGARGPNSATA
                     TACSASAHAIGDSFKIIERGAAEMMICGGTEATITAMGVGGFAAMKALSTRNDDPARA
                     SRPWDAGRDGFVVGEGAGILTLESLEHAQKRNAPILAEVVGYGMSGDAYHITQPAENG
                     DGAYRVMRAAIKDAKLSSDDIGYVNAHGTSTPIGDVIETRALKRLFGEKAKRVPISST
                     KSMTGHLLGGAGGLEAGISVLALRDQILPPTINLETPDPECDLDYVPNHARKASVEYA
                     LSNSFGFGGTNAALIFKRWSGK"
     gene            6879..7643
                     /locus_tag="VGR03_17885"
     CDS             6879..7643
                     /locus_tag="VGR03_17885"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEV1996209.1"
                     /translation="MNIIVCIKQVPAKDAPLAIAGNWIKESDIGFEMNEPDSYALEEA
                     LRLKEKHGGEVIALSMGPERVKQTIKEALAKGADRGIHIVDDNFAQLDPLGSAKCLAA
                     AIQKEKADLILTGLQSDDHGFGQTGVLLAGLLDLPHATLIMAIEVTDGKMKLKRELEA
                     GWFQHLECPLPAVLSIQSGINKVRYATLKGIMAAKKKEIATATRESLGVSFEATQKIE
                     KIYVPTKTKKTEFLTGTPKEVATKLVEKLKFEARVI"
     gene            7696..8685
                     /locus_tag="VGR03_17890"
     CDS             7696..8685
                     /locus_tag="VGR03_17890"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEV1996210.1"
                     /translation="MKILVITELRQGKWNNASFETLVAAQQIAKDTSSVVSALVIGKG
                     VAAFAAELAAKNVAEVLSVEHDLLEAYTPDGYCVALKQVIESAKPDLVLFPHTYQVRD
                     FAPKLAAMLGKGMIGDCIGYRNEGGKLVFVRQMFQGKTAADMTFAGAAPWFASFQSGA
                     FRSDLLAPHPSGKAPVNAVAVALNGEQIRTKPLDLFKEAKSAVDLTQAPLIVAIGRGI
                     KAPENIPQAEALAKALGGEIAASRPICDEGWLPMERQIGSSGQTVAPKLYLALGISGA
                     IQHVVGMKGARTIVAINKDANAPIFEIADYGVVGDIFEIMPALTEELQKAKRG"
     gene            8728..8934
                     /locus_tag="VGR03_17895"
     CDS             8728..8934
                     /locus_tag="VGR03_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV1996211.1"
                     /translation="MEDYKIILYRQENGSWVAEIPAIPGCYALMKTREEALAELPNVF
                     SMIEDEYREKGQKLPADSTEIVNA"
     gene            9186..10853
                     /locus_tag="VGR03_17900"
     CDS             9186..10853
                     /locus_tag="VGR03_17900"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEV1996212.1"
                     /translation="MTVQSEQLEADVLIVGAGPAGLACALHLANLIKKHNAAGGHSPG
                     AELSAENIYVLEKGREIGAHQLSGAIMNPKALAELVPDFEKSAPLDTLVTDDAALLFT
                     RGSSFRFPITPPPFKNKGNYVVSLSKVTKWLGGLVEAAGVNVFKEFGGAELVYEGDGI
                     AGVITEDKGVDKNGKPKDNYTPGYELRAKVTVLAEGTRGSLTKKLVEKKKLDNINPQS
                     YGIGIKELWDVQPGRIEPGYVAHTLGWPVSTDMYGGGWIYGLSNNRVSIGLVLALEYA
                     DPQFDPHAAFQTWKTHPFLKKLLDGGKLVRYGAKSLPYGGWYAMPRNYVDGGLIIGDS
                     GSFLDSQRLKGIHLAMKSGMLAAETIFDALKAGDTSSKTLSAFPKKIEQSYIKKELWQ
                     VRNFHQSFQHGMLGGFFHTAFQQISGGRGLIDPMRAHAGHEAYKKINGRAAPERFKGD
                     GKLTFDRLTDVYHSGTRHEEDQPCHLRVADTNICATRCVEEYGNPCQYFCPAAVYEMA
                     KETNGGVKLKISAANCVHCKTCDIADPYQIIDWVVPEGGGGPNYEGM"
BASE COUNT         2573 a         2827 c         3641 g         2199 t
ORIGIN      
        1 cggtgaaaca cacaacaaac aaagtggcta ggcagagtgc attccggaat cgcattgagt
       61 ttcctccaat tcgatttgga ccgggactcc gaaccagggc acgaaatgaa ccaaaggacg
      121 agaaatttcc aatcttccgg ataacacacg cgaaatcaaa gagatgcgcg gaatagcggg
      181 tatcagcgat gaagaaggat tgcgtaccag cgctatgaaa ttcttgccgc cctcgttttg
      241 ttccgtggag gattgaccct gcctgtgctt gaaagtagga tttggctgcg attcttgcga
      301 gttcgtggag cgaccaggag aaagtagagg agacactgtg tattgttcca agtgtggcgc
      361 agcgatggcg aatggtgcgg cgttttgctc gaattgcggt caagcgttcg cggttggccg
      421 gtgggacact gaattccttc cgaagttctt cctgatggca gcgcactggg aaaggggacc
      481 gctgctgcag ttcactacaa gatgaagaaa cgagggtgcc ctaccctttc gcgtttgtga
      541 aagtgcgggc tgttcttcgc ttggttcttt cgcccacgcc ccagctcatc ccaccggatt
      601 gctctcatcc tgactcttta ccgagtgtca ccaatcattc tgaacgagca cagtaacgtc
      661 gcgtaaaaat tgggatacaa gggggagacg tagataagga gtagaaggta ggcgtggata
      721 acgagcgctg gtactaggcc gggttggtga cgaagagggt gcaaaaacga gaagggggga
      781 ggggcgcttc cttgacttgg cggggaggcg actcaataat agtaaagcat gagcgtacgc
      841 gctggaggcg ctgaagagat gttccttgac gttaaggaat tagccgtccg caagctgcgc
      901 atccggaaga cctatgcccc gggcagcatc gattttcatt caccggagat caaacagatc
      961 gagccgctgg aagtgacggc cacggctgaa ttactggaat cgcagattcg catcgagggc
     1021 aacctcgaaa cgaagatcga attggtgtgt gcgcgctgcc tcgagcccgt ggtcgaagag
     1081 gtgagccgga cgtttgacct gttttacgcg cccctgccga aggaagcgaa gccgaccatc
     1141 gaccggctga aagacgacga caccgagatt ggattttacg aaggcccagg actctttttg
     1201 gcggatgtgt tgaaggagca ggtgctgctg gctctcccgt tgaaagtgat ctgccagagc
     1261 gactgccggg ggctatgccc gaattgcggt gcgaacctga accacgagga gtgccgctgt
     1321 gagacccacg cgacggacgc acgaatggcc cctctcgcgc gcctcaagca ggactggctc
     1381 aagaaacaat agttttccag ggctaacaag cccgatatca tccgcccact gaggcggcaa
     1441 cgaggtgtat tactatgcct aacccaaaac gacggcattc caagagacgg accagcacga
     1501 ggcgggcaaa cgactttttg accaagcccg cactggccaa gtgtccgaat tgccacgaaa
     1561 tgaaacagcc gcaccaggtt tgcccgcatt gcgggcagta caagggcaaa gcggcgcaag
     1621 aaccggcatc gtaaggattg cgagtttctc tcgcttagcc tgcggacaaa aatcccatga
     1681 tcaccatcgc tgttgacgcc atgggcggag acaatgcgcc ccgccccgag gtcgaaggca
     1741 gtgtgctggc cgcgcgggac ctgggcgtcc gcgtgctgct ggtgggccag cccagcgtgg
     1801 tgcgtgcgga gctcgccaag cacgcctcgc cgaatctgcc catcgagatc gtggcggcca
     1861 gcgaagtgat caccatgaac gatcaccccg cgcaggcctt ccgccggaag aaggacagct
     1921 cggtgcacgt ggcggcgcgg ctggtgcgcg aaacgaaagc cgatggcatg gtcagcgcgg
     1981 gaaacaccgg cgcagtgatg accgtggcga ggtttctgct gggcgcactg gagtccgtgg
     2041 atcgcgtggc gctggcggcg ccgtttccca acgccaaggg cggcgtttcc gtgctgctcg
     2101 atgtcggcgc gaatgtggat tccaagcccg agcacctcgt gcagtttgca gtgatgggcg
     2161 aggtttatta tcgcgcgacg tttggcaaca agcgcccgcg cgtgggattg ctctccgtcg
     2221 gtgaagaaga aatcaaaggc aacgaactga cgcgcgaggt ttacacgcgc ctgaagaaga
     2281 gcgcggtgca ttttgtcgga aacgtcgaag gcggcgatct tttttccggc aacgtggacg
     2341 tgattgtttg cgacggtttc gtgggaaaca tcgctttgaa gatttgcgaa ggactggcgg
     2401 tttcgatctt cgggctgctc cggaaatcgt taaaaagttc tttgatgtcc caggtcggtg
     2461 cgatgctttc gcagggtgcg ttcaagggct tgaagaagac cattgattac acggagtatg
     2521 gcggcgcgcc gctgctgggc gtgcgcggcg tttgcgtgat cgggcacggg cgttcgaacg
     2581 ccaacgccgt gaaaaacgcc attcgcgtgg ctgcggggct ggcccgcgcg cgcatgaacg
     2641 agaaaatcga acaggagctc tccgccgccg cggttatcgc gtgacggcgc gagagaagga
     2701 ttaacgatgg gcaaagtcgc attcgtgttt ccgggacaag cttcgcaata cccgggtatg
     2761 ggcaaagagc tggcggaaaa atatccggca gcgaaggccg tttttaacga agccgacaag
     2821 gcgctgggct tctcgatttc gaagatgtgc ttcgaaggta ccgaagaaga cctcaagctg
     2881 acggccaaca cgcagccggc gattttgacg tgctccgttg ccgtttaccg tgtgctggcg
     2941 gagaagggct tgacgccgga ttttgtggcg ggccacagcc tgggtgagta ttcggcgctg
     3001 gtggcggcgg gcgcgctgaa gtttgcggat gccgtacagc tcgtgcgcaa acgcggcacg
     3061 tacatgcagg atgcggttcc agcgggcgtg ggcgcgatgg ccgcaatcat gggattgtcg
     3121 ccggcggtgg tggcggacac ctgcaagcgc gcggcggaag gcgaaatttg ttcggcggcc
     3181 aatctgaatt cgccggagca gacggtcatc tccggtcacg cgagcgcggt gaaacgcgcg
     3241 gtagaaattg cttcgcagct cggtgccaag cgcgccgtaa ttctgccggt ttccgcgccg
     3301 ttccattcgg cgctgatgat gccggctcag gaaaagctgg aaatagactt gaagcagacg
     3361 gaatttgctg cgctgcgcgt gccgctggtg accaacgtgg atgcggacac cattgaaacc
     3421 ggcgacgaag cgcgcgaagc gctgatccgg caggtcacca tgccggtgcg ttgggaagag
     3481 tcggtgcggc tgctgattga tgagggcgtg aacacctttg tggaagtggg ccccgggcgc
     3541 gttttgtccg gattgctgcg gcaaatcgaa cgctccgtgg cgacactgaa cgtcgaagac
     3601 gaaaagagcc tctcggcgac ggtggagaaa atcgctggcg caaggtccga cgccgcctag
     3661 tagcgcaggc tttagcctgt gcaggctgga tttcaaccca gaggagacat ccacgggcga
     3721 aagcctgtgc caaggataat gttcagtcta aaagacaaag tcgcgctggt aactggagcc
     3781 tcgcaaggca ttggccgcga cacggcgcta gctctctccg aagcaggcgc gaaggtcgtc
     3841 gtaggggcgc gcaatgaaga aaagctcgca gcgctggttg tcgaaattgc cgcggccggt
     3901 ggcgaagcgt tcgcagtaaa gatggatgtg gccgacgccg aacaggtaaa ggccggattc
     3961 aagcaggtag tcgagaaatt tggacggctg gatattttgg tgaacaacgc ggcgatcacg
     4021 cgcgacggct tggcgctgcg gatgaagcag gacgattggg aagccgtgat tcgaacgaac
     4081 ttgaccggcg cgcacctgtg catccagcaa gcgctgcaca cgatgatgag ggcgcgcgcg
     4141 gggcggatta ttaacatcgc ctcggtcgtc gcgcagatgg gcaacgccgg ccaagcgaat
     4201 tacgtggcgg ccaaggcagg cttgatcggt ttgacgaagg ctatcgcaat ggagattgct
     4261 tcgcggaatg taaccgtgaa cgccgtcgcg ccgggcttca tcaagacgcc catgaccgat
     4321 gttttgcccg ataaagtaaa agaggagttg aacacgcgga ttccgctagg ccgcatgggt
     4381 tcggcgcgcg atgtggcagc ggcaatcgtg tttctggcca gcgatgaggc cggctacatc
     4441 accggccacg ttttggatgt aaacggcggg atgtatttgg cgtgagtctt ttgagaacat
     4501 ctgcggtgat atgtttgctt cttggctcgt gcgcagcttc gctcgcttcg gaagacgtcg
     4561 caaagactct tgctcaacat cctgtgtgga tagggcgcgg actgaaatgg gagcacatcg
     4621 aaggggaccc tgacccaaag cagactgttg ctggtgcgac agttctgtat ttgggcagga
     4681 acggaaagtt tgcaatggtg agtgggattg ttgttaagcg tgcggctgga atgatcataa
     4741 gcgaaggcga tggggaggcc gattctatcg gaacttgggc cgttcacgat gggactgttc
     4801 atgtgtccta tagattggtt tctaactata aaatagcgca caaggtggaa gaacctgaac
     4861 cgaaaattcc aggaccagtg gagaaagctg aaattagttt tgagcacaag cagggtgacg
     4921 acaaaaagcc ggtgacactc ttgcagtttc agggacacga atacgaaatt gcgcggggct
     4981 tgcccgtctc ggaaatgaag gaccggctcg aaatctataa agcgtcttca gcggcagaaa
     5041 ccttaagata gcaacggtcg agaaggtgac tgaggcgcac tctcttgaat ctagaggtaa
     5101 agttgaccta gcggggcggg gtcactagaa tgggcaggtt atccgggaga gcaattttag
     5161 ggtagtaggg ccgcaaccgc gccgggcggt cgcgaatctg agtaagcagg ctccaacgaa
     5221 cgagttttga cgaacgaccc gagcagcacc agcaggcagg gcacatcaag cgatagcaaa
     5281 tcgcggagga tacggaatgg ccagcgtgga agaacgcgtg aagcagataa tcgtcgagca
     5341 gctcggcgtg gatgaggcgg aagtgacgcc cacggcgtcg ttcgtggacg acctgggagc
     5401 ggattcgctc gaccaggtgg aattggttat ggcgttcgaa gaagcatttg gcatcgaagt
     5461 gcccgacgag gacgccgaaa agatgaccac cgtgaaggac gccgtcgaat acatcgacaa
     5521 acacgccaaa ggtaagtaag ccacggaaat ctcacctcaa cagggagcat cagaactttg
     5581 actcgccggg tagtcgttac gggcgttgga ttggtctgcg cgctgggcat cggcacggag
     5641 gaaacctgga agaacctcgt ggccgggaag agcggcgtcg ggccgattac ccatttcgat
     5701 accacgggat tcgactgcaa gatcgccggc gagatcaaga acttcgaccc cttccagtgg
     5761 atcgagaaaa aagaactgaa aaagatgggc cgctttatcc aggtggcgct ggccgccgcc
     5821 gattttgcgg tgaaaatgtc gggctggaaa cctgaaaact cggacctgga tgaagtgggg
     5881 gtctacattt cctccggcat cggcgggttc gatatcatcg agcgcgaaca catcaaactg
     5941 atgcagggcg ggccgggcag gatttctcct ttctttattc cttccgcgat tgtgaatctg
     6001 gcttcggggc atatttcgat tcgctacggc gcgcgcgggc cgaattccgc gaccgcgacg
     6061 gcgtgctctg cttccgcgca tgccatcggc gattcgttca agattatcga gcgcggcgcc
     6121 gcggagatga tgatctgcgg cggtacggaa gcaacgatta ccgcgatggg cgtgggcgga
     6181 tttgcggcca tgaaggcgct ttccacgcgc aatgacgacc cggcgcgcgc gagccgcccg
     6241 tgggacgcgg ggcgtgatgg atttgtggtg ggcgaaggcg cggggattct gactctcgaa
     6301 tcgctggagc acgcgcagaa gcgcaatgcg ccgatccttg ctgaagttgt cggctacggc
     6361 atgtccggcg atgcatacca catcacgcag ccggcggaaa acggcgatgg cgcgtaccgc
     6421 gtgatgcggg cggcgatcaa agacgcgaag ctgtcttcgg acgacatcgg ctacgtgaat
     6481 gcgcacggta cttcgacgcc gattggcgac gtcatcgaga cgcgcgcact caagaggctg
     6541 ttcggcgaga aagcgaaacg cgtccctata agctcgacga agtccatgac cgggcatttg
     6601 ctgggcgggg ccggcggact ggaagcgggc atcagcgtgc tggcgttgcg cgaccagatt
     6661 ctgccgccga cgatcaattt ggagacgccc gatccggagt gcgatctgga ctatgtgccg
     6721 aatcatgcgc gcaaagcgtc ggtcgagtat gcgctctcga attcgtttgg ctttggcggc
     6781 accaatgccg cgctgatttt caagcgttgg tctggcaaat agcatcccct tccgtgtgtg
     6841 ccgcaattgg gttttagatt cagtgaagga agaactccgt gaacatcatc gtgtgcatca
     6901 aacaggttcc cgcgaaggac gcgccgctgg cgattgccgg aaactggatc aaggaatcgg
     6961 acatcggttt cgagatgaac gagccggaca gttacgcgct ggaagaggcg ctgcggctga
     7021 aggaaaagca cggcggggaa gtgattgcct tgtcgatggg gccggagcgc gtgaaacaaa
     7081 cgattaagga agcccttgcg aagggcgcgg accgcggcat tcacattgtg gacgacaatt
     7141 tcgcgcagct cgatccgttg ggctcggcga agtgtctggc ggcggcgatt cagaaagaaa
     7201 aagccgattt gattttgacc ggcttgcaga gtgacgacca cggattcggg cagacgggcg
     7261 tgctgctggc cgggttgctg gatttgcccc acgcgacgct catcatggcc atcgaggtca
     7321 cggacggaaa gatgaagttg aagagggaac tggaggccgg ctggtttcag catcttgagt
     7381 gtccgctgcc ggcggtgctg tcgattcagt cgggcatcaa caaagtgcgc tacgcaacgt
     7441 tgaagggcat catggcggcg aaaaagaaag agattgcgac ggccacgcga gagtcgctcg
     7501 gtgtttcctt cgaagcgacg caaaagattg aaaaaattta tgtaccgacg aaaaccaaga
     7561 aaacggaatt tctgaccggc acgccgaagg aagtagcgac aaagctggtc gagaagttga
     7621 agtttgaagc gcgggtgatt tgatgtagcg ccggcatctt gccggtgttt ttcttcttcg
     7681 aaattgaggc gaaccatgaa gattcttgtt atcaccgagc tgcggcaagg gaagtggaac
     7741 aacgcgagtt tcgagacgct agtggcggcg caacaaatcg cgaaggatac atcgagcgtg
     7801 gtgagcgcgc tggtgatcgg gaaaggtgtg gcggcgttcg cggccgaact ggcggcgaaa
     7861 aacgtcgcgg aagttctgag cgttgagcac gatttgctcg aagcgtacac gccggatgga
     7921 tattgtgtgg cgctgaagca ggtaatcgaa agtgccaaac cggatttggt tttgtttccg
     7981 catacgtatc aggtgcgcga cttcgcgccg aaacttgcgg ccatgctcgg caaaggcatg
     8041 attggagact gcatcggcta ccgcaacgag ggtggcaagc tcgttttcgt gcggcagatg
     8101 ttccaaggga aaaccgcggc ggacatgacg tttgcgggag cggcgccgtg gtttgcttcg
     8161 ttccagtcgg gcgcgttccg ctcggatttg ctggcgccac atccatcggg aaaagcgccg
     8221 gtgaatgcgg tagccgtggc attgaacggt gagcagattc gcaccaagcc gctggatctt
     8281 ttcaaagaag cgaagtcagc ggtggatttg acgcaagcgc cgctcatcgt ggccattggg
     8341 cgcggcatca aagctcccga aaatattccg caggcggaag cgctcgcgaa agcgctgggc
     8401 ggcgagatcg ctgcgtcgcg cccgatttgc gacgaaggct ggcttccgat ggagcggcaa
     8461 atcggcagct ccggccagac cgtcgcgccg aaactttatt tagcgctggg gatcagcggc
     8521 gccattcagc acgtcgtcgg catgaagggt gctcgcacga tcgtggcgat taacaaggat
     8581 gcgaacgctc caatcttcga gattgccgac tacggcgtag tcggcgacat cttcgaaatc
     8641 atgcccgctc tcaccgaaga actacaaaaa gcaaagagag gatagccata agctacatct
     8701 aggcatatac tcacttctgt tatgagcatg gaagactaca aaataattct ctatcgtcag
     8761 gaaaatggat cttgggtagc cgagataccc gcgattcccg gatgctatgc tctgatgaag
     8821 acacgtgaag aagctcttgc cgaactgccc aatgttttca gcatgattga agacgaatac
     8881 cgcgaaaaag gccaaaagct tcccgccgat tccactgaga tagttaatgc ctagcgccac
     8941 ggcgcgcgaa tttcagcgcg tcgctcgcat actaggattc atactcatca gacaaaaagg
     9001 aagccacgcc agatggaacc atcccgacgg acgagctacg accattcccg tgcacggcgg
     9061 cgaggaaatt gggcctcctt tattcaacag agttctccgg caattgggca tcacactgga
     9121 tgaatttcag aagcttcgct aatagtaaaa tagcttttcg attttgctaa ggtttgaaaa
     9181 aagccatgac cgtacagagc gaacaactcg aagcggatgt gctgatcgtt ggcgcggggc
     9241 cggcgggtct ggcgtgcgcg ttgcatttgg cgaatctcat caagaagcac aatgcggccg
     9301 gcggacattc tccaggggcg gagttgtcgg ctgaaaatat ttacgtcttg gagaaggggc
     9361 gcgagattgg ggcgcaccag ctttcgggcg cgattatgaa tccgaaggcg ctggcggagt
     9421 tggtgccgga tttcgagaag agcgcgccgc tggatacgct ggtgacggat gatgcggcgc
     9481 tgctttttac acgaggatca tcgttccggt ttcccattac gccgccgccg ttcaagaaca
     9541 aaggcaatta tgttgtgtcg ctcagcaaag tgacgaagtg gctcggcggg ctggtggaag
     9601 ccgcgggcgt caacgtgttc aaagagttcg gcggggcgga gctggtgtac gaaggagacg
     9661 gcatcgccgg agtcattacg gaagacaagg gcgtcgacaa gaacggcaag ccgaaagaca
     9721 actacacgcc gggctacgag ttgcgcgcga aggtgacggt gctggccgaa ggcacgcgcg
     9781 gttcgctgac gaaaaagctc gtggaaaaga aaaagctcga caacatcaat ccgcagagct
     9841 acgggatcgg catcaaggaa ttgtgggacg tacagccggg gcgcatcgag ccgggctacg
     9901 tggcgcacac gctggggtgg ccggtttcca ccgacatgta cggcggcggc tggatctacg
     9961 gattatcgaa caatcgcgta tccatcggac tggttctcgc gctggaatac gcggatccgc
    10021 agttcgatcc acacgcggcg tttcagactt ggaaaacgca tccgttcctc aaaaagctgc
    10081 ttgatggcgg gaagctcgtg cgatatggcg cgaaatcttt gccgtacggc ggctggtacg
    10141 cgatgccgcg gaattatgtt gatggcgggc tgattatcgg ggattcggga agctttctgg
    10201 attcgcagcg gctgaagggc attcacctgg cgatgaagag cggaatgctg gcggcggaaa
    10261 cgatattcga tgcgctgaag gctggcgata cttcctcgaa aacactgagc gcattcccaa
    10321 aaaagatcga gcaaagctac atcaagaaag agctgtggca ggtgcggaat tttcatcagt
    10381 cgttccagca tggaatgctg ggtggatttt ttcacacagc ttttcagcag atcagcggcg
    10441 ggcgcgggtt gatcgatccc atgcgggcgc acgcggggca tgaggcgtac aagaagatca
    10501 acggaagggc agctcctgaa agattcaagg gcgatggcaa gctgacgttt gaccggctga
    10561 cggacgtgta tcactccggg acgcggcatg aggaagatca gccgtgccat cttcgcgtcg
    10621 ctgatacaaa catctgcgca acaaggtgcg tcgaggagta cgggaatccc tgccagtatt
    10681 tttgccccgc ggccgtgtac gaaatggcca aggaaacgaa cggcggcgtg aagctgaaga
    10741 tcagcgccgc gaattgcgtg cattgcaaga cgtgcgacat cgcggacccg tatcagatca
    10801 ttgattgggt ggtgccggag ggcggcggcg gcccgaacta cgaggggatg taaccgcgcc
    10861 tcttctcatt caaaacttca ttcctgtgac aaagggactc ccggcagaac ggttctaagt
    10921 tcttagtttt aatgtctagt cagacgaacc gcatcttgtc acgggcgcga tcgaaggcgg
    10981 gagtctccga aacccctttg atcccgcgca gctcttcaat gcggcgaacg gcacgggtct
    11041 ccttcgtctg cgctcaggac tagttcagac ctgctactac ggggcctatc caatggctgc
    11101 tctgcctgct ggcgagcgga tccagcatgg gggatgggag ggagtgcgta cctacccccc
    11161 ataatattga aagcatatga ttctatgaga gttaggatgt gggggtcggc aaagcatgtg
    11221 attccaaaag acttatttcg
//
LOCUS       DASYVO010000516         4264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_83917, whole genome shotgun sequence.
ACCESSION   DASYVO010000516 DASYVO010000000
VERSION     DASYVO010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83917"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VGR03_17905"
     CDS             complement(<1..484)
                     /locus_tag="VGR03_17905"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEV1996213.1"
                     /translation="MLLTIDVGNTNSVLGVFRGEELIANWRLTTAREQTIDEYGVLTR
                     NLFTLAGLDRDAITGVIISSVVPPVNRTLAEMSRVYFGKKALFVELGVKTGMAVLVDT
                     PSEVGADRIVNGVAAFHQYGGPCIVVDFGTAITFDAISAKGEYLGGVIAPGLGISSEA
                     L"
     gene            complement(485..1489)
                     /locus_tag="VGR03_17910"
     CDS             complement(485..1489)
                     /locus_tag="VGR03_17910"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEV1996214.1"
                     /translation="MAQESLPGTTDRRLAGLLALLAENATIVISGARIAKEIGVSRST
                     VWRWVERLRGLGVKVKGQTANGYFLEQVPDILTPDMLKQRLKGGLFGKRIFHFFKTDS
                     TNRVALELGHAGEPEGAVVLAEEQTAGRGRAGRKWHSERAAGIYVTLLLRPKLAPVQA
                     PLLTMMAGLSAHSAVQALTGLAVDLKWPNDLLIQGKKVGGILTEMHAEPGQIRFVIVG
                     IGLNVNQEKFPGELANIATSLRAETGKPQSRMELLVRLLREFENDYNRFLQEGVASVV
                     ARFESVSSYAKGKRVRITNGSESYAGTTAGLGPEGLLQVERDDGRMMTVIAGDVAEAR
                     "
     gene            complement(1525..2457)
                     /gene="nadC"
                     /locus_tag="VGR03_17915"
     CDS             complement(1525..2457)
                     /gene="nadC"
                     /locus_tag="VGR03_17915"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:NF013857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV1996215.1"
                     /translation="MDWNSEYIAGLVRRALEEDIGTGDATTGAVVPPRASAKAHIVAR
                     QTLVCAGLPIAEMIYRALDPEIRVTCPHNDGSFVEPGAEIVQIKGTSRAILTGERTTL
                     NFLAHLCGIATLTRRFVEQLAGTRTRIRDTRKTTPGLRTLEKYAVKTGGGANHRFGLY
                     DAILLKENHIALAGGIKAALDKAHTYASPKVPPPRAASAYDAAGLDPEVVGPGPLPVQ
                     IEVRDQKELLEALGAGAEAVLLDNMTPERAAECVAIARGIRAECVIEISGGITLDNAR
                     AYAEAGADFLSSGMLTHSAPAANLSLLVDSIEGT"
     gene            complement(2473..>4264)
                     /locus_tag="VGR03_17920"
     CDS             complement(2473..>4264)
                     /locus_tag="VGR03_17920"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEV1996216.1"
                     /translation="AAAGAYVGMDRFEARKKIVEDLKALGLLEKITEHTIAIGLCERS
                     KTIVEPRASTQWFCRMKPLAEPAIAAVERGEIQIVPDNRREEYFNWMRNIRDWTLSRQ
                     LWWGHRIPAWYCKENRHVTVARETPTKCATCGSTNLDQDPDVLDTWFSSGLWPFSTLG
                     WPENSPDFKRYYPTSLLITGYDILFFWVARMIMMGIHFTGKVPFRAVYLHSLVRTGSG
                     EKMSKSKGTGLDPVTLNQQYGTDAMRFCLASMAAPGTDIVLSEDKLTSARNFANKIWN
                     ASRFLFVNLDKFEESGTKLEELAAPEVRANAPYAYRGGVPLVDAWMFARLAATVELVN
                     EALANYRFHEAAQGVYQFFWGDFCDWYIEWVKPELQSADRERATAAWKNLFAAFEAAL
                     RLLHPFMPFLTEELWHQLPQKADAKSIALDQFPEAHEQWKNAKAMGDVDAIQEVVTAL
                     RNIRAELKLDPKKRVAADFSTSDASTGELVQANRGAIERFAVLSELRIVARQQFEAES
                     GAVRSTATFDVRIAYSDTVDVAAEKTRLNKEIEGLQNAIASKERQLGDEMFRSRAPEK
                     IIKGLEATLAEQRIEVQKLQDRLAQLEKDS"
BASE COUNT          812 a         1475 c         1069 g          908 t
ORIGIN      
        1 acaacgcctc cgacgaaatc cccagtccgg gcgcgattac cccgccaagg tattcgccct
       61 tcgcggagat ggcgtcgaaa gtgatcgccg tcccgaaatc cacaacgatg cacggcccgc
      121 cgtactgatg aaacgccgcc accccgttca cgatccggtc cgcgcctacc tccgacgggg
      181 tgtccaccag cacggccatc ccggtcttca caccgagttc cacgaacagc gccttcttcc
      241 caaaataaac tcgcgacatt tctgcaagcg tccgattcac cggcggcacc accgagctga
      301 tgatcacgcc cgtgatggcg tcccggtcca atcccgccag cgtgaacagg ttcctcgtca
      361 gcacgccata ttcatcaatc gtctgctcgc gcgccgtcgt caatcgccag ttcgcaatca
      421 gctcttcgcc gcgaaaaacg cccaacaccg agttggtgtt tcccacgtca atggtcagca
      481 gcatctaccg tgcctctgcc acgtcgcccg cgatcaccgt catcatccgt ccatcgtcgc
      541 gttctacttg cagcaaacct tccggcccga gtcccgcggt cgtaccggca tagctctcgc
      601 ttccgttcgt tatgcgcacg cgcttacctt tcgcataact cgatacagat tcgaagcgcg
      661 caacaacgct ggccacgccc tcctgcagga aacgattgta atcgttctcg aattcgcgca
      721 gcaaccgcac cagcagctcc atgcgcgatt gcggtttgcc cgtttccgcg cgtagggatg
      781 ttgctatgtt ggcaagctca cccggaaact tttcctgatt cacgttcaga ccgatcccca
      841 caattacaaa tcgaatttga cccggctcgg cgtgcatctc cgtcagaatg ccgcccactt
      901 ttttcccctg aatgagcagg tcgttcggcc acttcaggtc caccgccagt cccgtcagtg
      961 cctgcaccgc ggaatgcgcg gagagccccg ccatcatggt cagcagtgga gcttgtaccg
     1021 gggccagctt tggccgaaga agcaacgtca cataaatgcc cgcggcgcgc tcggaatgcc
     1081 atttccgtcc cgcccggcca cgtccggcgg tttgttcttc cgccagcact acggcgcctt
     1141 ccggctcgcc ggcatgtccc aattccagtg ccacgcgatt cgtcgaatcc gtcttgaaaa
     1201 aatgaaagat gcgtttcccg aaaaggccgc ctttcagccg ttgcttcagc atatccggag
     1261 tcaggatatc ggggacttgc tccaggaagt atccgttggc cgtctgccct ttcactttca
     1321 cgcccagccc gcgcagccgt tccacccacc gccatactgt cgatcgggat actccaatct
     1381 ctttcgcgat tcgtgcgccg ctgatcacga tggtggcatt ttccgccagc aacgcaagca
     1441 accctgccag ccgccggtcg gttgtgcccg gtaaggattc ttgagccatc gggatcttgc
     1501 cgcgcccctt cgcgccgcct aagcttacgt tccctcgatg ctgtccacaa gcaggctgag
     1561 gttcgcggcc ggcgcagagt gcgtgagcat cccggaagaa agaaagtcgg ccccggcctc
     1621 cgcgtaagca cgcgcattgt cgagcgtgat ccctccggag atttcgatca cgcattccgc
     1681 gcgaatcccg cgcgcgatcg cgacgcattc cgcggcgcgt tccggtgtca tattgtccag
     1741 cagcaccgcc tccgcccccg cgccgagagc ttcaagcaat tctttctgat cgcgaacctc
     1801 gatctgcacc ggcaacggtc ccgggccgac gacctccggg tccaacccgg ccgcatcgta
     1861 ggcgctcgca gcccgcggcg gcggcacttt tggcgaagca taggtatgcg ccttgtccag
     1921 cgccgcctta attcctccgg ccagcgcgat gtgattctct tttagaagga ttgcgtcata
     1981 gagtccgaac cggtggttcg cgccgccgcc cgttttcact gcatattttt ccagcgtccg
     2041 caaaccaggc gtcgttttcc tcgtgtcccg aatacgcgtt ctcgtgcccg ccagttgttc
     2101 cacgaagcgc cgcgtcagcg tggcgattcc gcaaagatgc gccagaaaat tcagcgtcgt
     2161 tcgctcgccg gtaagaatcg cgcgggaagt tcccttgatc tgcacgatct ccgcgccagg
     2221 ctctacaaac gatccatcgt tgtgcgggca ggtgacccgg atctccggat ccagtgcgcg
     2281 atagatcatt tcggcaatcg gcaatcccgc gcaaacgagc gtctgcctgg cgacgatgtg
     2341 agcctttgcc gacgctcgcg gtggtaccac cgccccggtc gtcgcgtctc ccgtgccgat
     2401 atcctcttcc aaggcacgcc gtacaaggcc agcaatgtac tcagaattcc aatccataag
     2461 accccgagag aactatgaat ccttttcaag ctgcgccaaa cgatcctgca gcttttgcac
     2521 ttcgatgcgc tgctcggcta gcgtagcttc cagcccctta atgatttttt ccggcgcgcg
     2581 gctgcgaaac atctcatccc cgagctgccg ttccttcgac gctatggcat tctgcaaacc
     2641 ctcgatttcc ttgttcaacc gcgtcttctc cgcagccaca tccaccgtgt cggaatacgc
     2701 aatgcgcaca tcaaaggtag ccgtggaacg caccgcccca ctctccgcct caaattgctg
     2761 gcgcgcaacg atgcgaagtt ccgacaaaac cgcgaacctc tcaatcgccc cgcgattcgc
     2821 ctgaaccagt tcacccgtac tggcatcgct cgtggagaag tccgccgcca ctctcttctt
     2881 gggatcaagt ttcagctccg cgcgaatgtt gcgcagcgcg gtgaccactt cctggatcgc
     2941 atcaacatcc cccatcgctt ttgcgttctt ccattgctcg tgcgcttctg gaaattgatc
     3001 cagcgcgatg gatttcgcat ctgccttttg cggcaactgg tgccacagtt cttccgtcag
     3061 gaagggcata aacgggtgca gcagcctcaa cgccgcttcg aacgcggcaa ataagttctt
     3121 ccacgctgcc gtcgctcgct cgcggtctgc gctctgcagt tccggcttca cccactcgat
     3181 gtaccagtcg cagaagtctc cccagaaaaa ctggtacacg ccttgagcgg cctcgtgaaa
     3241 tcggtagttt gccagcgcct cattcaccaa ctcgacggtc gccgccagcc gcgcaaacat
     3301 ccacgcatcc accagtggaa cgccgccacg ataagcatac ggcgcgttcg cccgcacttc
     3361 tggggccgcc agctcctcca atttcgtccc actctcttca aacttgtcca aattgacgaa
     3421 caggaatcgc gacgcattcc agattttgtt tgcgaaattc cgcgcgcttg tcagtttgtc
     3481 ctcggacagc acgatgtccg tccccggcgc ggccatggac gccaggcaga atcgcatcgc
     3541 atctgttccg tactgctggt tcagtgtcac cggatcgagt cccgtccctt tggacttgga
     3601 catcttttcc ccgctcccgg tacgcaccag cgaatgcaaa taaacggcgc ggaagggaac
     3661 tttgcccgta aaatgaattc ccatcatgat catccgcgcc acccagaaaa acaggatgtc
     3721 gtatccggtg atcagcaggc tcgtcggata gtacctcttg aaatccggcg agttctccgg
     3781 ccagcccagc gttgaaaacg gccacagtcc cgagctgaac cacgtgtcca aaacatcggg
     3841 atcctgatcg agattcgtag acccgcacgt cgcgcatttc gtcggcgtct cgcgcgccac
     3901 cgtgacgtgc ctgttctcct tgcagtacca cgccggaatc cggtgtcccc accagagttg
     3961 ccgcgacagc gtccagtcgc ggatgtttct catccagttg aaatattcct cgcgccggtt
     4021 atccggcacg atttgaatct cgccgcgttc caccgccgcg atcgcaggct ccgctagcgg
     4081 cttcatccta cagaaccatt gggtcgaagc ccgcggttcg acaatggtct tgctccgttc
     4141 gcacaagccg atggcaatcg tgtgctcggt gatcttctcc aaaaggccca gcgccttcaa
     4201 atcctcaaca atctttttcc gcgcctcgaa gcggtccatc cctacgtatg cgcccgccgc
     4261 tgcg
//
LOCUS       DASYVO010000517         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_152986, whole genome shotgun sequence.
ACCESSION   DASYVO010000517 DASYVO010000000
VERSION     DASYVO010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152986"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..>2464
                     /locus_tag="VGR03_17925"
     CDS             261..>2464
                     /locus_tag="VGR03_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996217.1"
                     /translation="MPEVQNDDPIVGKSYSTAILVSSFLLVLSLTWALYDEFFGLRPW
                     RSYQRHFTQAYERFLNQEIPKQQVALQAMEALPRFRELQAARDATQKAAAPRVTELDK
                     STAFVNQQIADISDAFTTARGKVQALTYQLELAPAGSGSRKSRQEDVDDAKKEKYSVE
                     LHTKDGKIEKKKLDFFELDKMFTDLKAEKASLTSDRIAALKDVNTLDIQIEEFKKAHM
                     TGLTPDQLRSLLRGAREMPEEIRQINVNPSNVALNNMGSGGLVDRCQSCHVATDSKVV
                     PPNLVLTKAALKLEKSNDAPFTSHPEPELIRIHSLDRFGCSPCHGGNGRAVSSVEKAH
                     GRYEHWLWPLYQRENFDSGCQQCHSADIVTEYAPVLNRGKDLFRSRGCVGCHKFEGFD
                     DQPEQLQATHQLIKQLGERRKEFELDVPRLNKQADAAPDNETARSLYAKANNLTVEMS
                     GIDARIEQLDLKSESLFREAKKVGPDLKEVRMKIHKEWIPVWLGHTHEFRPTTKMPQF
                     RLRTDEIEAISAYVWQSALPGPALPAQAAGNAARGKELLESRGCLACHSMGEGDKAMG
                     GTFAANLSRVGEKDKYEYLVRWIHNPRERTRPYCPYEKRDLGPEDYAKHGLPFVFDLD
                     HSRCPNDGHQLEVQQPTVMPNLRLTIEESRDIASYLLSKKHADASYASAPFMDDKSLM
                     EKGKGLIRNYGCAGCHEIAGFEEEGRIGTELTTEGSKPVDRLDFGRLTEIAKR"
BASE COUNT          558 a          736 c          756 g          414 t
ORIGIN      
        1 tgcaccgact gtgcatgctg acggagtttt ccagaaaact ctacaagcgg atgacgcttc
       61 tgcagatcat cacgctgcac acgctcctga ttctcatgtc ctcgctgccg gtaaaaatcg
      121 cgctgcggct gctcttccgg atcaagtaca tttgggtgac gaaatggttc agcatctgac
      181 catggcgggc tgacctttca tcgtgccgga agaagcgagg acttgctcct cattcgtgcc
      241 gaataccggg gaggtttgcg gtgcctgaag tccagaacga tgacccgatt gtagggaaat
      301 cctacagtac ggcgattctg gtcagttcgt ttctgctcgt gttgtcgctc acgtgggcgc
      361 tctacgacga attcttcggc ctgcgcccct ggcgcagcta tcagcgccat ttcacccagg
      421 cttacgagcg gttcctgaat caggagatcc cgaaacagca ggtggcgctc caagccatgg
      481 aggcattgcc gcgcttccgt gaactgcagg cagcgcgcga cgccacacaa aaagcggccg
      541 cgccccgcgt gacggagctc gacaagtcga ccgctttcgt caatcagcag atcgcggaca
      601 tcagcgacgc ctttacaacg gcccgcggca aggtgcaggc gctcacatat caacttgaac
      661 tcgccccagc gggctccggt tcgcgcaaga gccggcaaga agacgtggat gacgcgaaga
      721 aggaaaaata cagcgttgaa ctgcatacga aggacggaaa gatcgagaag aagaagctgg
      781 acttctttga gctcgacaag atgttcacgg atttgaaggc cgagaaagcc tctctgacct
      841 cggaccgcat cgccgctctc aaagacgtca acacattgga catccagatc gaggagttca
      901 agaaggcgca catgaccggc ctcacgccgg accagctccg aagcctgctc cgcggggcgc
      961 gggagatgcc ggaagaaatc cgccagatca acgtgaatcc atcgaacgtg gcgctgaaca
     1021 atatgggctc aggcgggttg gtggatcgct gtcaatcctg ccacgtcgcg acggattcaa
     1081 aagttgtgcc gccgaatctc gtcttgacga aggcggcgct caagctggaa aaaagcaatg
     1141 acgcgccgtt caccagccac cccgagccgg agctgatccg gattcacagc ctggaccgct
     1201 ttggctgctc gccatgccac ggcgggaacg gccgcgcggt ttcgagcgtg gaaaaggcgc
     1261 acgggcgcta cgagcactgg ctctggccac tctaccagcg ggaaaacttc gattccggct
     1321 gccagcaatg ccactccgcg gacatcgtca ccgagtatgc cccggtgctc aaccgcggaa
     1381 aggacctctt ccgatctcgt ggatgtgtcg gctgccacaa atttgagggc tttgatgacc
     1441 agccggagca gctccaggcc acgcaccagc tcatcaagca gctcggagag cggcgcaagg
     1501 agttcgagct ggatgtgccg cggctgaaca agcaggccga cgcggcgccg gacaacgaga
     1561 cggcacggag cctttacgcc aaggcgaaca acctgacggt cgagatgagc ggaatcgacg
     1621 cgcggatcga acaattggat ctgaagtcgg agagcctgtt ccgcgaggcg aagaaggtcg
     1681 ggccggactt gaaagaagtc cggatgaaaa tccacaagga atggattccg gtgtggctcg
     1741 ggcacacgca cgagttccgg ccaacgacaa aaatgccgca gttccgcctg aggactgacg
     1801 agattgaggc gatctccgcc tacgtctggc agagcgcact gccgggcccg gcgctgccgg
     1861 cgcaggctgc cggcaacgcg gcgcgcggca aggaactgct tgaatcgcgc ggctgcctgg
     1921 cttgtcactc tatgggcgag ggcgacaaag ccatgggcgg caccttcgcg gcgaatctct
     1981 cccgggtcgg cgagaaagac aaatacgaat accttgtgcg ctggatccac aatccgcgcg
     2041 agcgcacgcg gccctattgc ccttatgaga aacgcgacct cggaccggaa gactatgcca
     2101 aacacggctt gccgttcgtt ttcgatctgg accattcgcg ctgcccgaac gacggccacc
     2161 agctcgaggt gcagcaacca acagtgatgc cgaacctgcg gctgacgatc gaggaatccc
     2221 gtgatatcgc gagctacctc ctctcgaaaa agcacgcgga cgcgagctac gcttcggcgc
     2281 cgtttatgga cgacaagtcc ctgatggaga agggcaaggg cctgatccgc aactacggct
     2341 gcgccgggtg ccacgaaatc gcgggcttcg aggaggaagg ccgcatcggg acggagctga
     2401 cgaccgaagg aagcaagccc gtggaccgcc tggatttcgg ccggctgacg gaaatcgcca
     2461 agcg
//
LOCUS       DASYVO010000518         2901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_210983, whole genome shotgun sequence.
ACCESSION   DASYVO010000518 DASYVO010000000
VERSION     DASYVO010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210983"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2455
                     /locus_tag="VGR03_17930"
     CDS             <1..2455
                     /locus_tag="VGR03_17930"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEV1996218.1"
                     /translation="DMTGFTRRIGIGLLASALGCGTAVAQGWQHLSRVQRVEKMKYGV
                     ELTAGPAKVRITVFRDGLFRVRVAPNGTFPKDSSWAVIESPEPPALKIEENQKEIRIT
                     AGGVVATVQRSPLLINFSDAAGNVLLADEPSLPMAWNGQRVHAWKKMPLDENYYGLGD
                     KAGPMNRRNRAFTNWNTDAFGWQESTDPLYKTIPFFVGLRKGVAYGLFFDNTYRSSFD
                     FGKESADYFSFGAEGGELNYYFIAGPEPKKIIEQYTALTGRSPLPPLWTLGYQQCRYS
                     YYPEARAREIARLLREKKIPADTIYLDIDYQEGYAPFTINRQYFPHFEKMVADLRAQG
                     FHVILITDLHIKKNPGQGYAPYDSGTQNDAFVKNPDGSVYVGPVWPGDSVFPDFTLSR
                     VRDWWGGLYKDFAGMGAAGYWNDMDEPAVFRYPQKTMPLDVVHRLDDGSTLDHRAVHN
                     IFGMQNARATYDGLRKLQPDERPFVLTRAAYAGTQRFAATWTGDNLATWNHISMSTPT
                     LLSMGISGYPLVGDDIGGFGSSPPSDLLTRWYEVGAFNPIYRNHAAKGTLDHEPWVNG
                     PEQEAIRRKYIELRYKLLPYIYTIVEEMSRTGMPLMRPVFLQYPEAQEFYGDDRDFLF
                     GPDLFVAPVVSETADAHGVLLPPGEWYDYWTAEKHTNKDQINLHPRLDEVPLYVRAGA
                     ILPMQPVVQYTGERPNGPLELRVYLPTSAAGNDCRGALYQDDGHTFAYQRGEFLRVNY
                     SCQVSQSTVTVTTSVQKSGYQPWWNSVEVTLYGAPAVPKEVHVGDQLILKWRYDGQAQ
                     TVTFTLPNAVGSWTAHLTF"
     gene            complement(2475..>2901)
                     /locus_tag="VGR03_17935"
     CDS             complement(2475..>2901)
                     /locus_tag="VGR03_17935"
                     /inference="COORDINATES: protein motif:HMM:NF027968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HEV1996219.1"
                     /translation="IGMPGGGDPDNRRDFPGGWIGDSNDAFMQSGRTRDQQEIFSYVQ
                     SLLRLRREHAALRTGQLWHLASDESSYVFARETEEERIVIAFNNADRPRELRIPLADT
                     PAQRATAIGLLFGEARAELAGSEIRITMPAQSLSIFVLN"
BASE COUNT          618 a          868 c          805 g          610 t
ORIGIN      
        1 agacatgaca gggtttacgc gacgcatcgg aattgggctg cttgcctccg ccctcggatg
       61 tggaactgcg gttgcgcaag gttggcaaca ccttagcagg gtccagcgtg tcgaaaagat
      121 gaaatatggc gttgagttaa ccgctggccc agcgaaagtg cgcatcacgg tatttcgcga
      181 tggcctgttc cgcgtgcgcg tcgccccgaa tggtactttc cccaaggatt cttcctgggc
      241 agtgatcgaa tcgcccgaac caccagcact caagatcgaa gaaaaccaga aggaaatacg
      301 aatcaccgcg ggaggcgtag tcgcaaccgt tcagcgatca cccctgctga tcaacttctc
      361 tgatgctgct ggaaatgtcc ttctcgccga tgagccaagc ctgccgatgg cctggaatgg
      421 gcagcgggtt cacgcctgga agaaaatgcc gctcgacgaa aactactacg ggctgggtga
      481 caaagccgga cccatgaatc gccgaaatcg cgcctttaca aactggaata cggatgcctt
      541 cggctggcag gaatccaccg accctcttta caaaaccatt ccttttttcg tgggccttcg
      601 caaaggcgta gcctatggcc tgttctttga caacacctac cggagcagtt ttgacttcgg
      661 aaaggaatcc gcggactatt tttccttcgg cgcggaaggc ggagaactga attattactt
      721 catcgcagga ccggaaccga aaaaaatcat cgagcagtat accgcactca ccgggcgctc
      781 gcccctgccg ccgctctgga cgctcggtta tcagcagtgc cgctatagct actatcccga
      841 ggcccgcgcg cgggagatcg cccggctctt gcgcgaaaag aaaattcctg ccgacacgat
      901 ttacctcgac attgattatc aggaaggcta cgccccgttc acgatcaacc gccaatattt
      961 cccgcatttt gaaaaaatgg tcgccgatct tcgggcgcag ggcttccacg tcatcctgat
     1021 tacggatctt cacatcaaaa agaatcccgg ccagggctac gcaccctatg attccgggac
     1081 gcagaacgat gccttcgtga agaacccgga tggctcggtt tacgtcggcc ccgtctggcc
     1141 cggtgacagt gtctttcctg atttcactct ctctcgcgtg cgcgattggt ggggcggtct
     1201 ctacaaggat ttcgctggca tgggcgccgc cggctattgg aatgacatgg atgagcccgc
     1261 ggtttttcgt tacccgcaga agaccatgcc gctagacgtt gttcaccgcc tggatgacgg
     1321 ttcgaccctg gaccaccgcg ccgttcacaa catcttcggc atgcaaaacg cccgcgcaac
     1381 ctacgacggt cttcgaaagt tacaaccaga tgagcgccct ttcgtgctta ctcgcgctgc
     1441 ctacgccggt acgcagcgat ttgccgcaac ttggaccggc gacaatctcg ccacttggaa
     1501 tcacatttcc atgagtacgc cgacgctctt gagcatgggt atttccggat atccgctcgt
     1561 cggagacgac atcggcggat tcggcagctc gccgccgtcg gacttgctga cgcgctggta
     1621 cgaggtcgga gccttcaatc ccatttaccg caatcacgct gcaaaaggca cgctggacca
     1681 cgagccctgg gtgaacggtc cggaacagga agcgattcgc cggaaataca tcgagctgcg
     1741 ctataagctg ctgccctaca tctatacgat cgtggaagaa atgtcgcgca ccggcatgcc
     1801 tctcatgcgc cctgttttcc tgcagtaccc cgaagctcag gaattttatg gcgacgatcg
     1861 cgattttctc ttcggacccg atttgttcgt ggcgcctgta gtctcggaaa cggccgacgc
     1921 ccacggagtg ttgctgcccc ccggggaatg gtacgactat tggacggcgg aaaaacacac
     1981 caataaagac cagatcaacc tgcatccgcg tctggatgaa gtgccgctct atgttcgcgc
     2041 cggcgccatt ttgcccatgc aaccggttgt gcagtacacc ggcgagaggc cgaatggccc
     2101 gctcgaattg cgcgtctatc ttcccaccag tgcggcgggc aatgattgcc ggggcgcgct
     2161 ctatcaggat gacggccaca ccttcgccta tcagcggggc gagtttctcc gcgtcaacta
     2221 ttcatgccag gtttcgcaga gtaccgtgac ggtcacgacc agcgtccaga aaagcgggta
     2281 tcagccctgg tggaacagcg tggaagtgac tttgtatggt gctcctgctg tgccaaaaga
     2341 ggtccacgtc ggcgatcagc tcattctcaa atggcgctat gacggccagg cacagaccgt
     2401 cacgtttacc ttgcccaatg cggtagggag ttggaccgct cacctaacat tctgaccgct
     2461 gggcgcgcgg cggcctaatt cagtacaaaa atggatagcg attgcgccgg catggtgatt
     2521 cggatttcgc ttccagcaag ctccgccctt gcctcgccga agagaagtcc gatggcagtc
     2581 gctctttgcg ccggcgtgtc ggccaacgga atgcgaagct cccgtggccg atcggcattg
     2641 ttgaaggcga tgacaatacg ttcttcctct gtttcgcggg caaaaacata agatgattcg
     2701 tcggaggcca gatgccatag ctggccggtg cgcagggcgg catgttcgcg gcgcagcctg
     2761 agcagcgact gcacgtaaga gaaaatctcc tgttggtcgc gcgtccggcc ggactgcatg
     2821 aacgcatcgt tcgaatcacc aatccatccg cctgggaaat cgcgccggtt gtccggatcg
     2881 ccgccccccg gcatcccgat t
//
LOCUS       DASYVO010000519         2351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_84103, whole genome shotgun sequence.
ACCESSION   DASYVO010000519 DASYVO010000000
VERSION     DASYVO010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84103"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1682)
                     /gene="ltrA"
                     /locus_tag="VGR03_17940"
     CDS             complement(189..1682)
                     /gene="ltrA"
                     /locus_tag="VGR03_17940"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEV1996220.1"
                     /translation="MHESKKSDLAVVAVKPTNKAGGRSAAEPVEPRAGTEGNVGQQST
                     HRTQIRARVSQALERVRQAAKVRKKERFTALLHHIDLDLLRLSFYALARNAAPGVDGV
                     TWQDYEANLESNLLDLHWRVHRGAYRAQPSRRKYIPKPDGRQRPLGIAAVEDKILQRA
                     VVAVLNAIYEEDFLGFSYGFRPKRSQHDALDALIVGIQRKKVNWILDLDVRNFFGTVS
                     HEWLIRFLEHRIGDERILRLIRKWLKAGVLEEGIVTEGEAGTPQGATVSPLLANAYLH
                     YVFDLWAQQWRRRHATGDMIIVRYADDVVLGFEHEAEAKRFLEELRTRLQEYSLSLHP
                     DKTRLIEFGRHAAARREQRGLGKPETFTFLGFTLICGHTRKGQFQILRKTRSDRMRAT
                     LKKVKDELRRRMHLPIPEQEKWLQQVTTGFFAYHAVPTNSRALMAFRHHISVLWKRTL
                     SRRSQKATLTWERMLRLVKAWLPPPRILHPWPTQRFDVKHPRWEPSA"
BASE COUNT          448 a          759 c          654 g          490 t
ORIGIN      
        1 gagtaaccac acaaatgtcc tccggtccat cacgaagtaa cctcgtcgga cgaatcaaag
       61 tccaaccata ctacgccgcc cagctctgtt tcagccccaa cagtgagtga ccgcttctgg
      121 gcctattctg ttcgcggtag gaatgcgcat tgctgcgcac cccccgcaca gatccgagca
      181 agcgctattc acgcactcgg ctcccacctc gggtgtttga cgtcgaagcg ctgcgtgggc
      241 caagggtgaa ggatgcgcgg cggcgggagc caggctttga cgagtctcag catccgctcc
      301 cacgtcaagg tggccttctg gctacgccgc gacagcgtgc gtttccaaag taccgagatg
      361 tggtggcgga aagccatgag ggcccgggag ttcgtgggca cggcatggta ggcaaagaat
      421 cccgtcgtga cctgctgtag ccatttttcc tgttcgggga tgggtaggtg catccgccgc
      481 cgcagctcgt ccttcacttt cttgagcgtc gcccgcatgc ggtcacttcg ggtcttccgg
      541 agaatctgga attgtccctt gcgtgtgtgc ccgcaaatga gcgtgaaacc cagaaaggta
      601 aaagtctccg gtttaccaag accgcgttgc tcgcgccgcg ccgccgcatg gcggccaaac
      661 tcgatcagtc gggtcttatc cggatgcagc gacagcgaat actcctgcaa ccgcgtgcgc
      721 aattcttcca gaaaccgctt ggcctcagct tcgtgctcaa agcccagcac cacgtcatcc
      781 gcgtaacgga cgatgatcat gtcgccggtg gcgtggcgcc tgcgccactg ctgcgcccaa
      841 agatcgaaga cataatgcag gtaggcgtta gccaacaagg gggaaacagt agctccctgt
      901 ggcgtgcctg cctcgccttc cgtcacgatc ccttcttcaa gcactcctgc cttcagccat
      961 ttgcgaatca gccggagaat gcgctcgtcg ccgatccgat gttccaggaa acggatgagc
     1021 cattcatggc tgaccgtccc gaagaagttt cggacgtcga gatccaggat ccagttcacc
     1081 ttcttccgtt gaatgccgac aatcagcgca tccaatgcat catgctggct gcgcttcggc
     1141 cggaagccgt acgagaagcc caggaaatcc tcctcgtaga tggcgttgag caccgccacc
     1201 accgctcgtt ggagaatctt gtcctccact gcggcgatgc ccagcggcct ctgccgcccg
     1261 tccggcttgg gtatgtactt gcgccgacta ggctgcgcac ggtacgcgcc ccggtggact
     1321 cgccagtgca ggtctagaag attgctctcc agatttgcct cgtaatcctg ccacgtcacc
     1381 ccatccacac ccggcgccgc gttgcgcgcg agcgcgtaaa acgacagccg tagcagatcg
     1441 agatcgatat ggtggagcag cgcggtgaac cgttccttct tccttacctt tgcggcttga
     1501 cgtacgcgtt ccagcgcctg tgacacgcga gcccggatct gcgtccggtg cgtgctttgc
     1561 tggcccacgt ttccctcggt ccccgccctt ggctccaccg gctccgccgc cgatctacct
     1621 ccggctttgt tcgtcggctt cactgctact acggcgaggt cagacttctt ggattcgtgc
     1681 atcaccggct acggctcctc gccttcccgg cacggaccgg cagtgcgccg ccggccaaac
     1741 ccaagatctc ccggttcccg tacaagaagc gtctgcgcat gccagggtct tcgactacgc
     1801 cgggccgtcc ggatgctcgc catagcgcac ccgtccatgt tgctttccac gtttcaaacc
     1861 gtgtcggcac ccgggataag agtctttcgc agctcaatgg ctggcctgcg cgctcccctg
     1921 tcaacgcttc gctgacaccc tcacggatgc ccgcgcatga ctcggggtca ctgtggtttg
     1981 ctagaccttc agtgcagcgg acttgcacca cctactcctt gccggtctta ccggcgcact
     2041 tgaaaaactc gcttgcagcg gctgcatcga gtgtcgtcgc aagagtcgac cctctagaac
     2101 gcgctcagag cgacgcaaat gagtcaggtg atggtttgag acccccaaaa ttgccgcact
     2161 aaaagcgcga atcgagtttt tcaacagaat aggtggcggt cgttgaatga aatctgagtc
     2221 gctccgatct gacatcgtct gttcggtcgc atccaggtag ggagatctga accgacaatc
     2281 ggatcgcgcg cttcctgcgt cactcgaagc acttggcaca aagtacggac aacatggcga
     2341 gacgcttaag a
//
LOCUS       DASYVO010000520        12952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_192945, whole genome shotgun sequence.
ACCESSION   DASYVO010000520 DASYVO010000000
VERSION     DASYVO010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 12952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12952
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192945"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VGR03_17945"
     CDS             complement(<1..203)
                     /locus_tag="VGR03_17945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996221.1"
                     /translation="MSTNRIRTVLEMIKFEHSVFALPFALTGALLAARASHQGWPSLR
                     QILWIVVAMVAARSAAMTMNRIA"
     gene            complement(340..975)
                     /locus_tag="VGR03_17950"
     CDS             complement(340..975)
                     /locus_tag="VGR03_17950"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF004685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEV1996222.1"
                     /translation="MADTQKQIVTVGVTGASGAILAQKTLALLEEDARVAGVHLVVTE
                     AGQRLFTEELGITSGDLKQLPSRLLGHPAAKIDVFPNKDVGASIASGSCAVDAMVVIP
                     CSMGTLGAIANGISDDLVSRAADVMLKEDRKLVLCVRDTPFNRVHLENMLRAQQAGAV
                     IMPAVPAFYHQPKTIDDLVTQYVCRVLAQIGLSQDKMYRWTGHSASKEAKA"
     gene            1076..1843
                     /gene="larB"
                     /locus_tag="VGR03_17955"
     CDS             1076..1843
                     /gene="larB"
                     /locus_tag="VGR03_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEV1996223.1"
                     /translation="MNQAELLKILESVKAGKLAPDLAIERLKHLPFEDLGFAKVDHHR
                     ALRQGFAEVVLGKGKTPQQVAEIVRAMLRQKDSRQNILVTRADKKIFAAVKRAGGKDA
                     RYAKFHALSGAITIERIQEITGKGTILVVSAGTSDIPVAEEALLTARMMGSRVEHLYD
                     VGVAGIHRLLEHREKLTQARVIICVAGMEGALPSVVGGLVAIPVIAVPTSVGYGASFG
                     GVAALLGMLNSCASNVTVVNIDNGFGAACVASCINRL"
     gene            complement(1935..2945)
                     /locus_tag="VGR03_17960"
     CDS             complement(1935..2945)
                     /locus_tag="VGR03_17960"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1996224.1"
                     /translation="MLAFIVQGRRLVAKRKPLPELRKGWALIRVRLAGICNTDVEILR
                     GYHTFRGTPGHEFVGEVAEVHGVSAAIKIKWLGRRVCGEINVSCSGYGFERACNFCKR
                     GLKTHCARRTVLGIVNHDGAFSEYLALPLGNLHLVPKNVTDEQAVFVEPLAAACEILD
                     QVKVKKFRSAAVLGDGKLAQLIALVLRTAIPRVVIYGKHEKKLVIARHAGIQTKKVSG
                     DASDLRRIKESFGLLIEATGSSSGLGLAQHMTEPRGTLVLKSTFHGAAPVETWPIVVK
                     EITVVGSRCGPFGKAISLLRSGKLDPTPLITRTFPLTEAQKAIEYAQQRGVMKVLLKN
                     KL"
     gene            complement(2963..4393)
                     /gene="selA"
                     /locus_tag="VGR03_17965"
     CDS             complement(2963..4393)
                     /gene="selA"
                     /locus_tag="VGR03_17965"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEV1996225.1"
                     /translation="METRGKKTVSAEKAELLRQIPSVDELLAQPRLAALSKRADRNLV
                     VEVARAVLADLRARIAGDSNWTAISLDGASVEELISVQVEKLLSRSLQPVINATGVIL
                     HTNLGRAPLPETVVDEFRRTATQYSNLEYDLEGGARGKRDVHTAELLTRLTGTEAAIV
                     VNNCAAAVLVTLAALARGGEVIVSRGELIEIGDGFRIPEIMEQSGAILREVGTTNRTH
                     IADYENAINEKTRLLLRVHPSNFKVTGFTDKPSLEELVALSQRSGLPLVEDLGSGCLV
                     DLSESSVSEPTVKQSIDAGVSVVMFSGDKLLGGPQAGIIAGEKDLVARVRRYPLFRAL
                     RVDKLTIAALEATLGAYVRAAWEEIPAIRMIRATPQELKRRAENFIRELRPELPLDEV
                     EIEIADGSSLAGGGSTPSQSLPTKIIRIASARYSATKLEQRLRRAPAGISVIARVEDD
                     RLILDLRTVFPEQEPLLIKTLAAVLH"
     gene            4596..5207
                     /locus_tag="VGR03_17970"
     CDS             4596..5207
                     /locus_tag="VGR03_17970"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HEV1996226.1"
                     /translation="MATIDEELGTLERDIRQLKIEYDQYFGGGRKRPPTEIEWRIDQL
                     LKRYAERGGELKFAQRFRFNNLSQTYAKYKDIFRKRTAQKEEGKIQRHFGAAAKEIEA
                     ARAKKEAEEQAAAAAAGDSRTFRMTCSEPERETEKVDQLYQAFLQAKQTAGEETGKLS
                     RSSFNDFVLKKTKELQKQKNCKDVEYVVEVVEGQVKLKALVKS"
     gene            5298..6869
                     /gene="purH"
                     /locus_tag="VGR03_17975"
     CDS             5298..6869
                     /gene="purH"
                     /locus_tag="VGR03_17975"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV1996227.1"
                     /translation="MAVQRALISVFDKTGIVEFARRLAALKIEILSTGGTAKLLREAG
                     VAVREVSDFTGWPEMLGGRVKTLHPKVHGGLLFRRGHAEDQKQAAEHGIAPIDLVVVN
                     LYPFEATAAKAGLTAEELIENIDIGGPTMLRSAAKNFESVTVVTDPADFERVAGELES
                     AGETTLATRLELARKVFATTSRYDGMITTELERLFAGSGHVSLEAKPVLPERVHIALR
                     RQQELRYGENPHQAAALYVPAGRAPEGLAAAKQLQGKELSYNNLVDLEAARSLAAEFK
                     NPAAVIIKHNNPCGTAEQGTLLGAYLKALACDPVSAFGGVLAFNRVVDAATAEEVAKL
                     FVECIAAPGFADRAKEIFAAKKNLRLLELPPGGLEPERELQLKGILGGMLVQQPDLGE
                     IKDDELRTATKRAPTAHEMHTLRFAWKVAKHVKSNAIVFAKDGATLGVGAGQMSRVDS
                     VKLAVMKAQSSLAGTVVASDAFFPFPDGVEEAAKAGATAVIQPGGSVRDPDVIATADR
                     LGLAMVFTGMRHFLH"
     gene            complement(7513..9075)
                     /locus_tag="VGR03_17980"
     CDS             complement(7513..9075)
                     /locus_tag="VGR03_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996228.1"
                     /translation="MRLILHILKKDMRRHWPEILISLVLLGLYVWVTLQGPNNRFVGA
                     RFLWFQLSVESIPPLMIFFWIFLTVRVVQGETLVGDRQWWVTKPYEWWTLLAAKELFL
                     VVCFGVPLFFVQLYLMHHAGFPVFRNLLGILTMQFELGFVLFMPSVALGSLAKGLGQA
                     LVGIIVVIIGFWATSTLLEKVPSSGMSSAVRSSDDITGMMVLASIIGAVIWQYARRRT
                     WAARGLLVGGMGAVALISALTPYARFVDRKYPLVKASEAPAHFAVAQAKPSPKKQKDP
                     LDFTSDVYLRIPLLVSGIDEGRVVELDGIEISVETSSGAKWDPGWKSQWVQFWKEDDI
                     KSILYDVKRKAYERVKKENARLHIELALTEYQAGEVRELVLKDGEFSEPQLGICSLNE
                     KLSWQIDCRRPFQTPGLMATFDPSAAKCGADEDTEDLPEDRVSHAWFAPHSDNSPDAG
                     FNPVDKYQITLGSRRPFYTSGEKRKFKAVHLCTGARVNLAEPQEKRHVRVKLDMDNVR
                     LQDLLETGFDWD"
     gene            complement(9084..9980)
                     /locus_tag="VGR03_17985"
     CDS             complement(9084..9980)
                     /locus_tag="VGR03_17985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1996229.1"
                     /translation="MTAAIRTQELTKRFRSVRALDRVSLEVQQGAVYALVGPNGAGKT
                     TGIKILMNLIGATSGEAEVLGMDSKQIRGKAYKQIGYVSENQEMPEWMKVGSLLEYLR
                     KFYPSWDRELESALVKQFELPPARKIKALSRGMKMKLALASALAFHPKLIILDEPFGG
                     LDPLVRDQLIEGLLERAGEATVLLSSHDLAEIESFASHVGYLEEGRLRFSEEMSSLAA
                     RFREVEITFEGQSALPSPVPASWLQAKAAGGVVRFVESQFDHERTGAEIRQAFGNVKA
                     VAFRAMSLRAIFLAMARKAEAA"
     gene            complement(10019..10402)
                     /locus_tag="VGR03_17990"
     CDS             complement(10019..10402)
                     /locus_tag="VGR03_17990"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1996230.1"
                     /translation="MIPFRVAFRPGGTIYEQVVYAATKAMISGQLRPGDAFPSVRVLS
                     KELKINPNTAHKVIGYLVSAGLLEIRPGFGTVVAKRPQASRAEKTKLLEEQFEELVVE
                     GKRLGIALEDMEEAIEKHWKRLNSR"
     gene            10548..12176
                     /locus_tag="VGR03_17995"
     CDS             10548..12176
                     /locus_tag="VGR03_17995"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1996231.1"
                     /translation="MAIRVAVLGGGVGGLSAAHELIERGFEVTVYERRDVFGGKARSI
                     PVPNTGQGGRKDLPGEHGFRFFPGFYRHLPDTMMRIPYPGKTSVAENLVEATRIQLAR
                     AGQPPVILTARIPQDLEDWIVVFKEIFGGIGVPEDEVVFFGERLFILLTSCPERMDAE
                     YEQIPWWTFIDAANHSAAYQTLLGKGLTRSLVAVRAQEGSTRTAGRTLLQLLFGLMTI
                     GGFDRLLDGPTSDVWLTPWVNFLRKRGVDFQGGTTIQSLNATAAEIISVTVETNGQSR
                     QITADFYISALPVEIMTPLVGELLKTLAPSLANLGKLRTAWMNGIQFYLAQDVPLEFG
                     HSLYADSPWALTSISQRQFWSEVNLANVGDGKVGGILSVDISDWETPGILYGKKAMEC
                     TAKEIESEVWAQIKQHVNVGGAEVLRDDNLLDWFLDPDVQFPNPTAVTNLEPLMINTS
                     GSLQYRPEAFTEIPNLFLASDYVKTYTDVACMEAANEAARRAANALLDRAGSTAPRAT
                     LWPLREPEIFKPLIEFDRVRFLLGRPHASAKPGR"
     gene            12367..>12952
                     /locus_tag="VGR03_18000"
     CDS             12367..>12952
                     /locus_tag="VGR03_18000"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1996232.1"
                     /translation="MNLRQITLAIVLTGVAGAAAMAQTLQNPAPEKKSDSPAAAQEQL
                     TEPGTAPSAQESPRAEAYYNFTMGHIYEQQYEATSSAEYATKAIEAYKQAYALDPKSQ
                     VIGERLAEMYWKAQRIHDAVTEAQEILKRDPDNVQSRRLLGRIYLRSLGDVSASNGQS
                     ETVSHAIEQYREINRLDPSDTESALWLARLYRLKN"
BASE COUNT         2774 a         3709 c         3795 g         2674 t
ORIGIN      
        1 tcggcgatgc ggttcatggt catcgccgcg gagcgcgccg ccaccatcgc gacgacaatc
       61 cacaaaattt ggcgtagcga aggccatccc tgatggctag cgcgcgctgc gagcaacgca
      121 cccgtcagcg caaacggcaa agcaaatacg gagtgctcga acttgatcat ttcgagcacc
      181 gtgcgaatgc gattcgtgct catcggtaga aaaaagaatc gaacacagag tgcacggtga
      241 accccggccg aagcggcgaa attgccgacc cttctctgtg gactctgcgt tcggcgcctg
      301 ccccgacccg gtcggggtga tctctgtgtt gaaatctttt catgcttttg cttctttcga
      361 agcggaatgt cccgtccagc gatacatttt gtcctgcgag aggccgatct gcgccaggac
      421 gcggcaaacg tattgcgtaa cgagatcgtc gatcgttttc ggctggtgat aaaatgccgg
      481 caccgcgggc atgattaccg caccggcctg ctgggcgcga agcatgttct ccagatgaac
      541 gcggttgaac ggcgtatcac gcacgcacag aacgagtttt cggtcttctt tcagcatcac
      601 gtccgccgct cgcgagacca gatcgtcgct gatgccgttg gcaatcgcgc ccagcgttcc
      661 catcgagcag ggaatgacca ccatcgcatc cacggcgcag ctgccggaag cgatggaagc
      721 acccacatct ttatttggga agacgtcgat cttggcagcg ggatggccca gaaggcggct
      781 cgggagttgt ttcaagtcgc cggaggtgat gcccagctct tcggtaaaga gccgctggcc
      841 ggcttccgtg accaccagat gcacgcccgc cacgcgcgcg tcttcctcca gcaacgcgag
      901 cgtcttttgc gccagaatcg ctccgctggc gccggtcaca cctacggtga caatctgttt
      961 ttgtgtgtca gccatgtttt ctgcgcgggt agaattagtc tcgcagtggc gttcgggcaa
     1021 gtcaagctaa ggtgatcctc ccatgcgatg atagaattcg ctgcggccgg agaatatgaa
     1081 tcaagctgaa ttgctgaaaa ttctggaatc cgtgaaggct ggcaagctcg ctcctgacct
     1141 ggcgatcgag cgcctgaaac acttgccatt cgaggatctc ggctttgcga aggtggatca
     1201 tcaccgcgca ctacggcaag gttttgccga agtggttttg ggaaaaggga aaacaccgca
     1261 gcaggtggcg gaaattgtgc gcgccatgct gcgccagaaa gattcgcggc aaaacattct
     1321 tgtgacccgc gcagacaaaa agatttttgc agccgtgaag cgcgcgggcg gaaaagacgc
     1381 gcggtacgcg aagtttcatg cgttgtccgg cgcgatcacc atcgagcgca tccaggaaat
     1441 taccggaaaa ggaaccatcc tcgtggtttc cgcgggaacc agcgacattc ctgtagcgga
     1501 agaggcgctc ctcacggcgc gcatgatggg cagccgcgtc gagcatttgt acgacgtcgg
     1561 cgtcgccggc attcaccgct tgcttgagca tcgagaaaaa ttgacccagg cccgcgtgat
     1621 tatttgtgtt gccggaatgg aaggcgcgct tcccagcgtc gtcggcggcc tcgtagcgat
     1681 accggtcatc gccgttccca ccagcgttgg ctacggcgcc agcttcggcg gcgtcgccgc
     1741 attgctcggc atgctcaatt cctgcgcctc caacgtcacc gtcgtcaaca tcgacaacgg
     1801 tttcggcgcc gcctgcgtcg cctcctgcat caaccgcctc tagccctgta attcacccct
     1861 tcaggggtga ggtttttctc ctccttgttt gatcaggcat caaatcgaaa gagcctcagg
     1921 ccttaaggcc tgagctacag tttgtttttc aacaagactt tcataacgcc cctttgctgg
     1981 gcgtactcaa tggctttctg cgcttccgtc aggggaaacg tgcgcgtaat gagcggtgtg
     2041 gggtcgagtt ttccggagcg caataacgag atggctttgc cgaacgggcc gcagcgggag
     2101 ccaaccaccg tgatttcctt aacgacgatg ggccaggttt cgactggcgc cgcgccgtga
     2161 aacgtggatt tgaggaccag agtgccccgc ggctcggtca tgtgttgcgc caaacccaat
     2221 ccacttgacg agccggtagc ttcgatcaag agcccgaagc tttctttgat ccgtctcaga
     2281 tcgctggcgt cgccgctgac tttttttgtt tgaatgccag catggcgggc aatcaccagt
     2341 tttttctcat gcttgccata gatgacgacg cgtggaattg cggtgcgtag cacaagcgcg
     2401 atcaactgtg ccagcttgcc gtcgccgagg acggcggcgg agcgaaactt tttcactttg
     2461 acttgatcca ggatttcgca ggcggcagcg agcggctcga caaagacggc ctgctcatcc
     2521 gtcacgtttt tcggaacgag gtgcaggttt cccagcggca aagccagata ttccgaaaac
     2581 gccccatcgt ggttcacgat ccccagaacc gttcgccgtg cgcaatgcgt tttcaatcct
     2641 cgcttgcaaa agttgcaggc cctttcgaat ccatagcccg agcaagaaac attgatttct
     2701 ccgcagaccc ggcggccgag ccattttatc tttatggccg ccgaaacgcc gtgcacctcc
     2761 gcgacctcgc cgacaaattc atggccgggc gtcccccgaa aagtgtggta gccgcggaga
     2821 atctcaacgt ccgtgttgca aatgccagca agtcgaacac gaatgagcgc ccagcccttt
     2881 cgcaattctg gcagcggctt tctcttggct acaagtctgc ggccttgaac gatgaacgcg
     2941 agcattcaac ccttttcaat catcagtgca gcacagcagc cagggtctta atcaatagcg
     3001 gctcctgttc gggaaagacc gtgcgcaagt cgaggatgag gcggtcgtct tcgacacggg
     3061 cgatgacgga aatgccggcg ggagcacggc ggaggcgctg ttcgagcttt gtagcggaat
     3121 aacgggcgct ggcgatgcgg atgatttttg taggcagaga ttgcgacgga gtagagccgc
     3181 caccggcgag agaactgcca tcggcgattt cgatctcgac ttcgtcaaga ggaagttcgg
     3241 ggcgcagctc gcggatgaaa ttctccgcgc ggcgcttcag ttcctgcgga gtcgcccgga
     3301 tcattcgaat ggcggggatt tcctcccagg ccgctcttac gtaagccccg agagtggcct
     3361 ccagcgctgc gatggtgagt ttgtccacac gcagcgcgcg aaacagcggg tagcgccgga
     3421 cgcgagcgac gagatctttt tcccccgcga tgatgcctgc ctgcggaccg cccagaagtt
     3481 tgtcgccgct gaacatcacc acggaaacgc ccgcgtcgat gctttgcttc accgtgggtt
     3541 cgctgacgct ggactcggaa agatccacga gacagcccga gccgaggtct tccacgagcg
     3601 gcaggccgct gcgctggctg agggccacga gttcttcgag cgaaggctta tcggtgaagc
     3661 cagtgacctt gaaattcgaa ggatgcacgc gaagcaacag gcgcgtcttt tcattgatgg
     3721 cattttcgta gtccgcaatg tgcgtgcgat tggtggtgcc gacttcgcgc aggatcgcgc
     3781 cgctctgctc catgatctcg ggaattcgaa agccgtcgcc gatttcgatg agctccccgc
     3841 gcgagacgat tacttcgccg ccgcgagcca gcgccgcgag agtcacgagc acggccgcag
     3901 cgcaattgtt caccacaatg gcggcttccg tgccggtcag gcgcgtcagc aattcggcgg
     3961 tatgcacgtc ccgtttgccg cgcgcgcccc cctcgagatc gtattcgaga ttgctgtatt
     4021 gcgtggcggt gcggcggaat tcgtccacca ccgtttccgg cagcggcgca cggcccaggt
     4081 tcgtgtgcag aatcactccc gttgcgttga tgacaggctg aagcgagcgc gagagaagct
     4141 tctccacttg gacggagatg agttcctcaa cggaggcgcc gtctaaactg atggcagtcc
     4201 aattcgaatc gcccgcaatt cgcgcgcgca aatcggcaag caccgcgcgc gcaacttcga
     4261 ccaccaagtt gcggtccgcg cgtttcgaaa gcgccgccag ccgcggctgc gccagcaatt
     4321 cgtccaccga cggaatctgc cgcagcaatt ccgccttctc cgctgacacc gttttcttcc
     4381 cgcgcgtttc cattcgaaca tttcagcaca ctggcgggtc cccggcaatg ccgctggcct
     4441 tctcctggcg tctcgccccc ggcatgagcg ttggcccgca acctgtctcc agccgaaggc
     4501 agagtgcggt accggattcg aatcgccgcc cactcattcg gttgacggtt ttgctccgta
     4561 cacctagcat ggagactcga gggattcctt cacgcatggc gaccatcgac gaagagctgg
     4621 gcacgctcga acgggatatc cgtcagctca agatcgaata cgaccagtat ttcggcgggg
     4681 gcaggaagcg tccgcccacg gaaatcgagt ggcgcatcga tcaactcctc aagcgctacg
     4741 cggagcgtgg cggcgagctc aagttcgcgc agcgattccg cttcaacaat ctttcgcaga
     4801 cctacgcgaa atacaaagat attttcagga aaagaacggc gcagaaagaa gaaggaaaaa
     4861 tccagcggca tttcggcgcg gccgcgaagg aaattgaagc agcccgcgcg aaaaaagaag
     4921 ccgaagagca ggcggctgca gccgcggcgg gagactccag gacatttcgc atgacttgtt
     4981 ccgaaccaga acgcgaaacg gagaaggtcg accagttgta ccaggcgttt ctgcaagcca
     5041 agcagacggc cggagaagaa acgggaaagc tgtcgcgctc cagcttcaac gattttgtgc
     5101 tgaagaaaac gaaagaactg caaaagcaga aaaactgcaa agatgtcgaa tacgtcgtgg
     5161 aagtggtcga aggccaggtc aaattaaaag ctctcgtgaa gtcgtaagcc tctcccggcg
     5221 tttcccacag cgtttgtact tcccctattc gcgctagccc cttacactgt tgtgcttttc
     5281 tcattgtgga ggaatccatg gcggtacagc gcgcgctcat cagtgtgttt gacaagaccg
     5341 ggattgtgga attcgcgagg cggctggccg cgctgaagat cgagattctc tcgacgggcg
     5401 ggaccgctaa gctgctgcgc gaagcgggtg tcgcggtgcg cgaagtttcc gatttcacgg
     5461 gctggcccga gatgctaggc ggacgcgtga agacgctgca cccgaaagtg cacggcggac
     5521 tcctcttccg tcgcgggcac gcggaagacc agaagcaggc ggcggaacat ggaatcgcac
     5581 cgatcgatct ggtggtggtg aatctgtatc cgtttgaagc gacggcggcg aaagcgggac
     5641 tgacagccga agagttgatc gagaacatcg acatcggcgg gccgacgatg ctgcgatcgg
     5701 cggcgaagaa tttcgagagt gtgacggtgg tgacggaccc tgcggatttc gagcgcgtgg
     5761 ccggtgaact cgagagcgcg ggcgaaacga cgctggcgac gcggctggag ttggcgcgaa
     5821 aagttttcgc gacgacttcg cggtacgacg gcatgatcac aacggaattg gagcgcttgt
     5881 tcgctggatc ggggcatgtt tctttggaag cgaaaccggt gctgccggaa cgcgtgcata
     5941 tcgccctgcg gcggcagcag gagttgagat atggagaaaa tccccaccaa gccgcggcgc
     6001 tgtacgtgcc ggcgggccgc gcaccggaag gattggcggc ggcgaagcaa ctgcagggca
     6061 aggaactttc gtacaacaat ctcgttgatc tggaagcggc tcgcagcctg gccgcggaat
     6121 tcaagaatcc agcggcggtg atcatcaagc acaacaatcc ctgcggaaca gcggaacagg
     6181 ggacgttgct gggagcgtat ctgaaagcgc tggcgtgcga cccggtttcg gcgttcggcg
     6241 gcgtgctggc gttcaaccgc gtagtggatg ctgcgacagc ggaagaggtc gccaaactgt
     6301 ttgtggagtg catcgcggcg cccggttttg cggatcgcgc caaggaaatc tttgcggcga
     6361 agaaaaacct gcggctgctg gaactgcccc cgggtggctt ggaaccggaa cgcgagttgc
     6421 agctcaaggg cattctcggg ggaatgttgg tgcagcagcc ggatctgggc gagatcaagg
     6481 acgatgagct gcgcacggca acgaagcgag cgccaacggc gcacgagatg cacacgctgc
     6541 ggttcgcatg gaaagtggcg aagcacgtga agtcgaatgc aatcgtgttt gcaaaggatg
     6601 gcgcgacgct gggagtcggc gcagggcaga tgagccgcgt cgattccgta aaactcgcgg
     6661 tgatgaaggc gcaatcttcg ctggccggta ccgtggtcgc ttccgacgcg ttctttccgt
     6721 ttccggatgg ggtcgaggaa gccgcgaaag cgggcgcgac ggcggtgatc cagccgggcg
     6781 gctcggtgcg cgatcccgat gtgatcgcaa cggcggaccg gcttggactg gcaatggtgt
     6841 tcaccggcat gaggcatttc ctgcactgag attccgtgac tggtgactgg tacaagaaaa
     6901 gagtagggcc tcagccctga agggctgagc tacaggcacg ggagagtgat ttctagttaa
     6961 caataacacc cccacccccc ctcttttttg gaagtgcgga atctaaagta cttagcaatt
     7021 ctgtaagtcc tttgtttgcc acacatccga agagcgccgg aagtgtggta tctaaaggag
     7081 ttagggggat agtgggatag cggagcaggg gaataggaat cggagaagaa tcctttccga
     7141 caaggtcgga atggtccatc gactcccgat ggtcgctcct cgagatgctc ggggcaggca
     7201 ggatgcgcat cctggccgga tgcgcacagc ggaagtatcc acctcccccc tatgtttttg
     7261 taagaatgtg acacgaaagg agttagctgg tgggggtcgg ctaaaagtgt gagtataaag
     7321 aagttagagg cgggtcccgc agtggagcgg gaattcaggg cggatccgcg agagtggtgg
     7381 ctgacgatca caactcatgt tagcaatgta gtacagtact agtaaagata aatatagaag
     7441 tgctttagac agaaggagat agcgcgaggc ggagcgacgg gggcggagag caactaacag
     7501 acacctggac ccctagtccc agtcaaaccc ggtttcgagc aaatcctgaa gtctcacgtt
     7561 gtccatgtct agtttcacac gcacgtgcct cttctcctgg ggctctgcca ggtttaccct
     7621 ggctcccgtg cacaagtgca cggccttgaa ttttcttttt tctccggagg tatagaacgg
     7681 cctcctggat ccaagagtaa tctgatattt gtccacgggg ttgaatccgg catctgggga
     7741 attgtcactg tgcggcgcga accaggcgtg tgacacgcgg tcttcgggca gatcttccgt
     7801 gtcttcgtct gctccgcact tcgctgcgct gggatcaaac gtagccatta aacctggagt
     7861 ttgaaacggc cgccggcagt cgatctgcca agaaagtttt tcattcaagc tacagatccc
     7921 gagctgcggt tccgagaatt cgccgtcctt tagaaccagt tcccgcactt cccctgcttg
     7981 gtattccgtc agcgccagtt caatatgcag ccgggcgttc tcctttttga ccctttcgta
     8041 agccttgcgt tttacatcat agagaataga tttgatatcg tcttccttcc agaattgaac
     8101 ccattgcgat ttccagcccg gatcccattt cgctccgctg gatgtttcaa cagagatttc
     8161 tattccatcc agctccacca cgcgtccttc gtcaatacct gataccaata agggtatacg
     8221 taaatagaca tcggatgtga agtccaacgg gtccttttgt tttttcggac tgggcttcgc
     8281 ctgtgcaact gcgaagtgtg ccggcgcctc gcttgctttg acgagcggat atttccgatc
     8341 cacaaatctg gcgtaaggcg tgagcgcgga gatcaatgcg actgccccca tgccgcccac
     8401 aagaaggccg cgcgccgccc aggtcctgcg ccgagcgtac tgccaaatga ccgcaccgat
     8461 gatagacgcc agaaccatca ttcccgtaat atcatcggaa cttcttacgg ccgacgacat
     8521 tccgctgctg ggaacttttt caagcaaggt agacgttgcc cagaatccga taatcaccac
     8581 gatgattcca accagggctt gtcccagccc tttcgccagg ctcccgagcg cgacactggg
     8641 catgaaaaga acgaaaccca gctcaaattg catcgtaaga attcctaaga ggttgcggaa
     8701 aacggggaag cccgcgtggt gcatcagata caactgcaca aaaaacagag gcacaccgaa
     8761 acaaacaacc aagaacaatt ccttcgccgc gagcagggtc caccattcat aaggtttcgt
     8821 cacccaccac tgccgatcgc ccaccaaggt ttcgccctga accacgcgca cggtaagaaa
     8881 aatccaaaaa aagatcatca acggcgggat cgattccaca gagagttgaa accagagaaa
     8941 ccgggcccca acgaatcgat tgtttggacc ctgaagcgtg acccacacgt aaagccccaa
     9001 caacaccagg gagatcagaa tctccggcca gtgccgccgc atgtccttct tgagaatgtg
     9061 gagaatcagc cgcatgccgc cccctaagcc gcttcagctt ttctggccat agccaggaaa
     9121 attgcccgca gactcatcgc tcgaaaggcc acggccttga cgttcccaaa cgcctgccgg
     9181 atctccgctc cggtcctctc gtgatcgaac tggctctcga caaagcgcac cacccctccc
     9241 gcggctttcg cttgcagcca gcttgccggt acgggcgacg gcagtgcgct ctgtccttca
     9301 aaggtgattt cgacttcccg gaatcgcgcc gccaaggacg acatctcttc cgaaaagcgc
     9361 agccttcctt cctccagata tccaacgtgg cttgcgaaac tctcaatttc cgccaggtca
     9421 tgagatgaaa gtaacacggt tgcctctccc gcccgctcca gcagtccctc aatcaactga
     9481 tcccttacga gcggatccag acctccgaac ggctcatcca ggatgataag cttgggatga
     9541 aaggcaagag cgctggccag cgccagtttc atcttcatgc cgcgcgacag cgccttgatt
     9601 tttcttgcag gaggcaattc aaattgtttg accagggcag actctagctc ccggtcccag
     9661 cttggataga actttctgag gtactcgagg agagaaccga ccttcatcca ctccggcatt
     9721 tcctggttct cggaaacgta gccgatctgt ttgtaggctt tcccgcgaat ctgtttggag
     9781 tccattccca gcacttccgc ttctccgctg gttgccccga tcaggttcat caagattttg
     9841 atcccagtcg tttttcctgc gccgttaggc cccacaagcg catagactgc accctgctgc
     9901 acctcaagac ttacgcggtc gagcgcgcgc acacttcgga atcgcttggt cagttcctgg
     9961 gttcgaatcg ccgccgtcat ctctcgcgcc ctccttgact cccctccggg tttgccgcct
    10021 atctgctgtt caaacgcttc cagtgcttct cgatcgcttc ctccatgtcc tcgagcgcaa
    10081 tccccagccg ttttccttcc accacgagct cctcgaactg ttcctcaagc agcttggtct
    10141 tttcggccct gctcgcctgt ggcctctttg ccacgacggt tccaaatccg gggcgaattt
    10201 ccaaaagacc tgcggagacc aggtagccaa tcaccttgtg ggccgtgttg ggattgattt
    10261 tgagttcctt gctcaacacg cggaccgaag ggaacgcgtc gccgggacgc aattggcccg
    10321 agatcatcgc tttcgttgcg gcgtagacca cttgctcgta gatggtgccg ccggggcgga
    10381 aggccacgcg gaacggaatc atgtgcgtac tataacaaat agtacactga aagcaatagc
    10441 tgcctttcag gtgatgttga ccgagttgga cgcggacatt aggtgagcgg ggaggctctg
    10501 ccccggcctc ttgtctggca tcaatactgc ggatagagta acgcgccatg gcgatccgtg
    10561 tggcggtcct gggcggcggc gtgggcgggc tgagcgcagc gcatgagcta atcgagcgcg
    10621 gcttcgaagt gacggtctac gagcgaaggg atgttttcgg cgggaaagct cgcagcatcc
    10681 ccgttcccaa caccggccag ggtggccgaa aggatttgcc gggcgagcac ggcttccgct
    10741 tctttcccgg gttttaccgg cacctgccgg acaccatgat gcggattcct tatccaggaa
    10801 aaacaagcgt agctgagaat ctggttgagg caacacgcat tcaattggcg cgtgcaggcc
    10861 agccaccggt catcctgaca gcgcgcattc cgcaagacct cgaggattgg attgtagttt
    10921 tcaaggaaat tttcggtggg atcggagttc cggaagatga agttgtcttc ttcggagaac
    10981 gcttgttcat cctgctgacg agctgtccgg agagaatgga tgctgaatac gagcaaattc
    11041 catggtggac gtttatcgat gccgccaatc attcggcggc ctaccagacg cttttgggca
    11101 agggcctgac gcgttctctc gtcgcggtcc gcgcgcagga gggcagcacg cgcacggcgg
    11161 gccgcactct gctgcagttg ctattcggac tcatgacgat tgggggcttc gaccgactgc
    11221 tggatggtcc caccagcgat gtgtggctga ccccgtgggt gaacttcctg cggaagcgcg
    11281 gcgtcgattt ccagggcgga acgacgatcc agtccttgaa tgcaacggcg gcggaaatta
    11341 tctccgtcac cgtggaaacc aacgggcaat cgcgacaaat cacggctgat ttttacattt
    11401 cggcccttcc ggttgagatc atgacgccgc ttgtcggtga actgttgaag actctcgcgc
    11461 cttcgcttgc gaacctcggc aagcttcgca cggcatggat gaacggcatt cagttctatc
    11521 tggcgcaaga cgtgcccctg gagtttggac actcgctgta tgccgattca ccttgggcac
    11581 ttacgtccat atcgcagcgc cagttctgga gtgaggtcaa cctggcgaat gtcggtgacg
    11641 gaaaggtggg cgggattctc tcggtagaca tttcggattg ggagacgccc ggcattctat
    11701 acggcaaaaa ggccatggaa tgcacggcca aggaaatcga aagcgaggtg tgggcgcaga
    11761 tcaagcagca cgtaaacgtg gggggcgccg aggtgcttcg cgacgataat ctactcgact
    11821 ggtttctcga tccggacgtg caatttccga atccgacggc ggtcacaaat ctcgagccat
    11881 tgatgatcaa cacgtcagga tcgctgcaat atcgcccaga agcattcacg gagattccca
    11941 atctttttct ggcttccgat tacgtgaaga cctacacgga cgtcgcgtgc atggaagcgg
    12001 ccaacgaggc tgctcggcga gccgccaacg cgcttctcga ccgggccgga tcgactgccc
    12061 cgcgagccac gttatggcct ttgagagaac cggaaatctt caagccgctc atcgaattcg
    12121 atcgcgtgcg gttcctgctc ggccgaccgc acgccagcgc caaacccgga cgctgaagtg
    12181 tcgttgtcgg cttcgaagat ttctttagaa atttctcagg atgtgaggag ttgaattttt
    12241 cactttcaac gtttttccgc taagctgcat cctagctacg ggggcgccca agctctagaa
    12301 acgttttgta tttggctttt gaccgcgttt cggccgcatg ggataattta ggtttctggg
    12361 gctacgatga accttcgaca gattactctt gccatagtgt tgacgggcgt tgccggtgcg
    12421 gctgccatgg cgcagacgct gcagaatccg gcgcctgaaa agaagtccga ctctcctgcc
    12481 gcggcgcagg agcagctaac cgaaccggga accgcgcctt ccgcacaaga atccccgcgt
    12541 gccgaggcct actacaactt cacgatgggc cacatctacg aacagcaata cgaagcgacc
    12601 agcagcgccg aatatgccac gaaggcaatc gaggcgtaca agcaagcgta tgcgcttgat
    12661 ccgaaatcgc aggtcatcgg cgagcggctg gcggagatgt actggaaggc gcagcggatt
    12721 cacgatgcgg taaccgaagc gcaggaaatc ctgaaacgcg acccggacaa cgtgcaatcg
    12781 cggcgactgc tggggcggat ctatttgcgg agcctgggcg atgtcagcgc gagcaacggc
    12841 cagtcggaaa cggtcagcca cgccatcgag cagtaccgcg agattaaccg gctcgatcct
    12901 tcggacaccg aatcggcgct gtggctggcg cgtctttacc ggctgaagaa cg
//
LOCUS       DASYVO010000521         4407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_153231, whole genome shotgun sequence.
ACCESSION   DASYVO010000521 DASYVO010000000
VERSION     DASYVO010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153231"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="VGR03_18005"
     CDS             complement(<1..1306)
                     /locus_tag="VGR03_18005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEV1996233.1"
                     /translation="MAASTDEFPQGKNPEKFNIAEHFLDAPAALHPERIAIAGEPREV
                     SYGELAALVNRAGNALRAQGVSRGDRVLIVLPDLAEFVAVFFGAAKIGAVAVPVNPFA
                     RSSDYIHYLENSEPRAAIIHSEALAEFLPASSVLPQIPIVVVGEETRDLHGVACAKWS
                     AWTAAASEQLVAANTSPQDPAFMLYTSGSGGTPKAAVHRHADMLATSRNYAQSVLGLR
                     ADDLTFSVSKLFFAYGMGNGMYFPLSAGARTLLNPQRTNVAHVIEMVARHRPTVFFAV
                     PTFYAAVLRETERPDNRVDFSSVRMCVSAGETLPAEIFNAWKRQFGIEILDGIGSTEM
                     LHIFISSVPGKCKPGSCGFPVPGYEVKIVDDDGEYVSDGEIGNLWVRGASAFAEYWRI
                     PELTARTKRGDWVVTGDKFFCDSEGYYHYCGRADDMLKVAGMW"
     gene            complement(1334..1807)
                     /locus_tag="VGR03_18010"
     CDS             complement(1334..1807)
                     /locus_tag="VGR03_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996234.1"
                     /translation="MFETPEEAKQKEPKGFRIAIAVVVAVVAVGTYFFVRSRGNINKQ
                     ASAASASAVVKGNADPVHDLKIQRATMNKDRNGTMAVWLVTIENKSQVYSYSKIEYET
                     TYVGADNNAILINKGTLPGTIAPGEQKKSEINDALYPAGTAWYKIRITGATPAAH"
     gene            complement(2514..2771)
                     /locus_tag="VGR03_18015"
     CDS             complement(2514..2771)
                     /locus_tag="VGR03_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996235.1"
                     /translation="MQRRLPACITSAQTKILAIAGLWQTSLAMVTSNRNMGRLSLRLT
                     HHPAMQATLDISWRLIIRYWQAKQESKVPSSRGWPLIQPVT"
     gene            complement(3004..>4407)
                     /locus_tag="VGR03_18020"
     CDS             complement(3004..>4407)
                     /locus_tag="VGR03_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996236.1"
                     /translation="RDASSSTTPNGIHPDQHALVFMPGNPEVWFEGSDGGLMRSSGMY
                     TDISSQCAGRGLSPDKILLCQRLLSSVPTLLTSLNTGLDTLQFQSLSINPLKPTRELL
                     GGTQDNGTFLYEGSSVLWPQTIFGDGGQSGFNAANTDIRFHTYFIQQVDVNFRGTDTL
                     GWDWVADPLFEDPIENSSFYIPIIADPNLAKAGSMFAGLQGVWRTTDNGGDQTTLDPH
                     CNEFTGDFTVRCGDWVELGSLTGHNTPASDLTHASHGNRSGGVIAAITRALSDTATLW
                     VATSRGRVFVSKNADAVSTVSGADGSSVTFSRIDSLVVNSPGRFVSGIAVDAAKPNHA
                     WISYSGYNMNTPAQPGHVFEVTYDPVAGTAAWTDLDGGTGPMGDLPVTALVRDDPTGD
                     LYAATDFGVLRLPAGGTAWQTAGSGLPQVEVPGLSISTSARVLYAATHGRGAYVLHLR
                     ELDNKGGEHARGEGGDH"
BASE COUNT          943 a         1262 c         1275 g          927 t
ORIGIN      
        1 cccacatccc cgccactttc agcatgtcgt ccgcgcgccc acagtaatgg taataaccct
       61 ccgaatcgca aaagaatttg tcgccggtca ctacccaatc gccacgtttc gtgcgcgccg
      121 tcaattcggg aatccgccag tactccgcaa acgcgctcgc gccacgcacc cagagattgc
      181 caatctcgcc gtcactcacg tattctccgt cgtcatccac gatcttgact tcgtaacccg
      241 gcacggggaa accacaactg cctggcttgc attttccggg aacgctcgag atgaaaatat
      301 gcagcatttc cgtcgagcca attccgtcga ggatctcgat tccgaattgc cgcttccacg
      361 cgttgaagat ttcagcgggc agcgtttccc cggctgatac gcacatgcgc acggaagaaa
      421 aatccacgcg gttgtccggc cgctccgttt cccgcaacac tgccgcataa aaagtcggca
      481 ccgcgaagaa aacggtcggc cggtggcgcg ccaccatctc gatcacatgc gccacgttgg
      541 ttcgctgcgg gttcaacagc gtccgcgcgc ccgctgaaag cgggaaatac attccgtttc
      601 ccatgccgta ggcaaaaaat agtttcgaca cagagaacgt caggtcgtca gcgcgcaggc
      661 cgagaacgga ttgcgcataa ttccggctgg tggccaacat atccgcgtgg cgatgcaccg
      721 ccgccttggg cgtaccgccg ctgccagatg tgtaaagcat gaaagctgga tcttgagggg
      781 aagtgttcgc cgccacgagt tgttcgctgg ccgccgctgt ccacgcactc cacttcgcgc
      841 aggctacacc gtgcaggtct cttgtttcct cgccgacgac gacaatcgga atttggggga
      901 gcacgctcga ggccggcaaa aactccgcaa gcgcctcgga atgaataatc gccgcacggg
      961 gctcgctatt ctcgaggtaa tgaatgtagt cggaagatcg cgcgaaggga tttacgggca
     1021 cggccaccgc gccgattttt gcggcgccaa aaaaaacggc gacaaactcg gccaaatccg
     1081 gcagcacaat cagaacacga tcgccccggg aaacgccttg cgcccgcagc gcattccccg
     1141 cgcgatttac gagcgcggct agttcgccgt agcttacttc gcgcggttcg ccagcgatgg
     1201 cgatgcgctc ggggtgcaac gcggccgggg catccaagaa gtgctcggca atgttgaact
     1261 tctctggatt tttaccctgc gggaattcat ctgtggaagc agccatcgtt ctcgattcga
     1321 aacgaacttc gggctaatga gccgcgggag tcgcgccagt gattcgaatc ttgtaccagg
     1381 ccgtgcccgc cgggtagagc gcgtcattga tttcggactt tttctgttcg ccgggggcga
     1441 tggtacctgg aagtgtgccc ttgttgatca gaatggcgtt gttgtcggcg ccgacataag
     1501 tggtctcata ctcgatcttg ctgtagctgt acacctggga tttattctcg atggtcacga
     1561 gccagacggc catcgttccg ttgcgatcct tattcatcgt cgcgcgttgg atcttcaaat
     1621 catggacggg atcggcgttc cccttgacca ccgcggacgc gctggcagca gatgcttgct
     1681 tatttatatt gcctctggac ctgacaaaga aatacgttcc aacggcaacc acggcgacaa
     1741 ctacggcgat agcaatccgg aatcctttcg gctctttctg ctttgcctct tcgggcgtct
     1801 caaacataaa ttcctccgac tccctgagcc cgcgacactc ttgcaacaga tccccgatgg
     1861 cctccgacga aaaagatgaa ctgcctacca agggaactcg cgattgtgcc agggaatggt
     1921 tcggcacttt tcaatgccgg agcgccttag agatagcact gaaggcgcgg gaagtccatc
     1981 gttgagaagg gacggtagcg ggtcagtttc ctgtaagccg actttacgac caagttacag
     2041 cgaacgatag tttccgagtg ggcgacaatc cgggagttga tcggttcggt caacactacg
     2101 gcgtttatgg ggagtaagct accagcggca gccagggcgc gccgaaaacg tgtcggcgca
     2161 tttgtggtaa ttgtctgcgt tgggctgcta gggcctgttg ggttcccgta ctgtggagtg
     2221 tgcggggacg aaatagtttc gacaatacag ctcaaatcac ctcgggttgc cggctcgctc
     2281 gtgattgtgc gcgcgtctcg gcatgaccgg actagtggtt ataaccaggt tcagtgggga
     2341 acttccactc acggtagcag ccacaggctt agtaaccgga tatccggcaa gagcgaacgt
     2401 cacggacaca gaggcagttt gggtggcgcc ggtcgggaca aatttccaat tcttcaaact
     2461 ctgtatcacc ggtccagcga gaagcggatc accagacgga attttaactt catctatgtt
     2521 accggctgga tcaatggcca gccgcgcgac gacggtacct tgctttcctg cttggcttgc
     2581 cagtagcgga taatcaggcg ccacgaaata tctaacgttg cctgcatggc agggtgatga
     2641 gtcaggcgca gggacaggcg gcccatattc cgattgctcg tcaccattgc gaggctcgtc
     2701 tgccacaagc cggcaatcgc aagaattttg gtttgcgcag acgtaatgca tgccgggagg
     2761 cggcgctgca tgatcgcaga tagattgttg atgatgtata tatattatgg taaagaacgt
     2821 caacgctaat ggagccaatt tcaacatatc tccctcccag gttcgcggat acccgcgcaa
     2881 tgttttcaag cggaagatgt tgccggcggc ctatctttag aacgtagctg gagacattcc
     2941 ttgcatccac ctgcaagaaa aaaaagaggc cgccctcggg cggcctcctg gtggcggttg
     3001 tggctagtgg tctccgccct ctcctcttgc atgctcgccg cctttgttat caagttcacg
     3061 caaatgcagg acgtaggccc cgcgcccgtg ggttgcagca tatagcacac gcgcagaggt
     3121 agagatagaa agccctggaa cctcgacctg cggcaggcct gaacccgccg tctgccacgc
     3181 cgtgccaccg gccggcaggc gcagaacgcc gaagtcggtg gctgcgtaca gatcgccggt
     3241 cgggtcgtcg cgaaccagcg cagttaccgg caggtctccc atcggaccgg tgccgccatc
     3301 caaatcggtc catgccgctg tcccggcaac cgggtcatag gtgacctcaa acacatggcc
     3361 gggttgcgcg ggtgtgttca tgttgtagcc ggagtaggag atccaagcgt gattcggctt
     3421 cgctgcatcc acggcgatgc cgctcacgaa acgtcccggc gaattgacca cgagagaatc
     3481 gattcgcgaa aacgtaactg agcttccatc cgcgcccgaa acagtcgaaa cggcatccgc
     3541 attcttggaa acaaagacgc ggcccctgga agttgccacc caaagcgtgg cggtgtcgct
     3601 gagggcgcgg gttattgcgg ctatgactcc accggaacga ttaccgtggg aggcgtgagt
     3661 gaggtcgctt gccggagtgt tgtgtccagt cagactacca agctcgaccc aatctccgca
     3721 ccggacagta aagtcgccgg tgaactcgtt gcaatgcggg tcgagggttg tctggtcgcc
     3781 gccgttatcc gttgtccgcc acactccctg aaggccggca aacatcgagc ctgccttcgc
     3841 cagattggga tcagcgataa tgggtatata gaatgaggaa ttttcgatgg ggtcctcaaa
     3901 cagaggatcg gccacccagt cccagcccag agtatccgtc ccacggaagt tcacgtccac
     3961 ctgctgtatg aagtaggtgt ggaaacggat gtccgtgttg gcggcgttaa atccggactg
     4021 gccgccgtct ccgaagatgg tttgcggcca aagtacgctg gatccttcat ataggaatgt
     4081 gccgttgtcc tgagtgccgc ccaacagttc tcgagttggc ttaagcgggt tgatagaaag
     4141 actttggaat tgcaaggtgt ccaatccggt attgaggctg gtcagcagcg tcggcacgga
     4201 ggagagcagg cgctggcaga gcaggatttt gtcggggctc aatccgcgcc ctgcgcactg
     4261 gctcgaaatg tcggtgtaca tgccgctgga acgcatcaaa ccgccgtctg acccttcaaa
     4321 ccatacctcg ggattaccgg gcatgaacac cagagcgtgc tgatcgggat ggatgccgtt
     4381 gggagtggtg ctcgaagagg catcacg
//
LOCUS       DASYVO010000522         3483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_211202, whole genome shotgun sequence.
ACCESSION   DASYVO010000522 DASYVO010000000
VERSION     DASYVO010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211202"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VGR03_18025"
     CDS             <1..449
                     /locus_tag="VGR03_18025"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEV1996237.1"
                     /translation="TIVLDDKDITKSTVNVTIDVSTVDTREPDRDNDLRSEKFFDVAH
                     FPTMTFKSKKVEQVATGKLKVTGDLTIRSTTKEVVLDVEGPTAPVKDPWGNVRAAATA
                     TSKVNRQDFGVKWNATLDNGGVVVGDDVNITIDAEMIKQAPAKSGN"
     gene            538..1161
                     /locus_tag="VGR03_18030"
     CDS             538..1161
                     /locus_tag="VGR03_18030"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV1996238.1"
                     /translation="MEGIEFMHKPAPSDFPVHELIRERWSPRAFADKPVPQNILSSIF
                     EAARWAPSSSNEQPWAYLVASRDDKDSFEKILSVLVEFNANWAKGAPVLAIAVAELAF
                     AKNNAPNRNAQYDTGAASAMLSVEATSQGLAVHQMAGFDPEKAREVFGIPAGWDAIAA
                     LAIGYPGDPASLPQPLKDREMAPRTRKPIAEFVMAGHWGHTAPFATK"
     gene            1188..1454
                     /locus_tag="VGR03_18035"
     CDS             1188..1454
                     /locus_tag="VGR03_18035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996239.1"
                     /translation="MHRTLEFLLHHGYALLLGWVFAEQIGLPLPSMPLLLAAGALAGT
                     GHLNFFASLFYVMLAAVAADSIWYQLGRRKGIRILQLLCKISLD"
     gene            1447..1998
                     /locus_tag="VGR03_18040"
     CDS             1447..1998
                     /locus_tag="VGR03_18040"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEV1996240.1"
                     /translation="MIEPDSCVRRTEGVFSKQGARSLLLAKFLPGLSTVAPPLAGIFH
                     MRPRRFLFFDAAGSLLWAGTFLGLGYVFSGQIERVAEHLASLGGWLLVLLLGALAAYI
                     AYKFVARQRFLRELRISRITVDELKEKLDSGEELVIVDLRHSMDFEADPGTIPGAFRM
                     DSKELEEKNDRLPRDREIILYCT"
     gene            complement(2277..2609)
                     /locus_tag="VGR03_18045"
     CDS             complement(2277..2609)
                     /locus_tag="VGR03_18045"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEV1996241.1"
                     /translation="MRILIDECAPRALKRHLINHGHECLTVQESGWSGKQNGELLSLA
                     EISFDVLVTVDTSLRYQQNLSGRRIAVVILQSSSNRLGHLRQYFPACVLAIEKIKPGE
                     IVQVGSAT"
     gene            complement(2606..2827)
                     /locus_tag="VGR03_18050"
     CDS             complement(2606..2827)
                     /locus_tag="VGR03_18050"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEV1996242.1"
                     /translation="MATVSESVIIKDPNILGGEPVFRGTRVPFKVLTDYLEGGDTLDQ
                     FLEEYPSVSRELAVAAIEEARLSLVAQLK"
     gene            complement(3005..>3483)
                     /gene="dcd"
                     /locus_tag="VGR03_18055"
     CDS             complement(3005..>3483)
                     /gene="dcd"
                     /locus_tag="VGR03_18055"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEV1996243.1"
                     /translation="VREGVISYGVSSYGYDIRVADEFRIFTNVNSTIVDPKHFDPKSF
                     VEFRGDVCIIPPNSFALARSVEYFRIPRNVLTICLGKSTYARCGIIVNVTPFEPEWEG
                     FVTLEISNTTPLPAKIYANEGLCQVIFFESDEDCEVSYKDKKGKYQSQSGIVLPRL"
BASE COUNT          730 a         1096 c          916 g          741 t
ORIGIN      
        1 gcacgatcgt gctggacgac aaagacatca ccaagtccac cgtcaacgtc accattgacg
       61 tctcgaccgt ggatactcgc gagcccgacc gcgacaacga cctgcgcagc gaaaagttct
      121 tcgacgtcgc ccacttcccc accatgactt tcaaatccaa aaaagtcgag caggtcgcta
      181 ctggaaagct caaagtcacc ggcgatctca ccatccgcag caccaccaag gaagtcgtcc
      241 tcgacgtgga aggcccgacg gctcccgtga aagatccctg gggcaatgtg cgcgcggccg
      301 ccacggccac atccaaggtc aaccgccagg acttcggcgt gaagtggaac gccaccttgg
      361 acaacggcgg tgtcgtcgtc ggtgacgacg tcaatatcac tatcgatgcg gaaatgatca
      421 agcaagcgcc ggcgaagtcc ggcaactgac gacaccctgt cagtgcggtt tgggaaagga
      481 gtggccggcg cggatgcacc gtccactccc cctgaatcat caaagaagag attgcaaatg
      541 gaagggatcg aattcatgca caaaccagcg ccaagcgatt ttccagtcca cgaactgatt
      601 cgcgagcgct ggagcccgcg ggctttcgcc gacaaaccgg ttccacaaaa catcctgagc
      661 agcatcttcg aggctgcccg gtgggcgcct tccagtagta acgagcagcc ttgggcgtat
      721 ctcgtcgcgt cgagagacga caaagacagt tttgagaaaa tcctgagtgt tctcgtggaa
      781 ttcaatgcca attgggccaa gggcgctccc gtgctggcga tcgccgtcgc ggaattggca
      841 ttcgcgaaga acaacgcccc caaccgcaac gctcaatacg acaccggcgc ggcatcggcc
      901 atgctctccg tggaagcaac ctcacaggga ctcgccgttc atcagatggc cggatttgat
      961 ccggaaaaag cccgcgaggt ctttggaatt cccgccggat gggatgccat cgccgcactc
     1021 gccatcggtt acccgggcga cccagcttcc ttgcctcagc cgctgaagga tcgcgagatg
     1081 gcgcccagga cacgcaagcc gattgccgaa tttgttatgg ccggtcactg ggggcatact
     1141 gctcctttcg caacgaaata gccggagatc gctgaccccg ccccgccatg caccgcacac
     1201 ttgaatttct gctccatcat ggatacgcat tgttgctcgg atgggttttc gccgagcaga
     1261 tagggctccc gctcccctcg atgccgcttt tgctcgcggc gggcgcgctc gcgggtaccg
     1321 gccatctgaa ttttttcgcc tcgctgttct acgtcatgct cgcggccgta gcagcggatt
     1381 ccatctggta tcagcttggc cgccgcaaag gcatcaggat cctccagctt ctctgcaaga
     1441 tttcgcttga ttgagccgga ttcctgcgtt cgccgcaccg agggcgtttt ttcaaagcag
     1501 ggcgcgcgct cgcttctgct ggccaagttt ctccctggtc tcagcacggt cgcacctccc
     1561 cttgcgggca tctttcacat gcgcccgcgg cgtttcctct tcttcgatgc cgcagggtcg
     1621 cttctctggg ccggcacgtt cctcggcctc ggttatgtat tcagcggtca gatcgagcgc
     1681 gtagccgagc atctcgcctc tctcggcggc tggctcctgg ttcttctcct cggcgccctc
     1741 gcggcctaca tcgcgtataa atttgtcgcc cgccagcgct ttctgcgcga actgcgcatc
     1801 tcccgcatca ccgtggatga attgaaggaa aaactcgact ccggcgaaga actggtcatc
     1861 gttgacctgc gccactcgat ggacttcgaa gccgatccgg gaaccattcc cggcgccttc
     1921 cgcatggact ccaaggagtt ggaagagaag aacgaccgtt tgccgcgcga ccgcgaaata
     1981 atcctctact gcacttgacc aaacgaagcc accagcgccc gtgtggcgct gctccttagg
     2041 aagaatggca tcacccgcat ccgcccgctt caaggcggcc tggatgcctg gcgcgaacgc
     2101 ggctaccccc tcgacaccgc cacgctcccc taatcccgca agaaggatgc gccccggctt
     2161 tcctgggctc cattttcgcc aatcgtacta tctggcgcgc tagtaatgca ggccagtttc
     2221 cgtgtcggat tcacggcagg tctgcgttct ttgagtccga gcttgcattg ggggtatcag
     2281 gtcgcacttc cgacttgaac gatttccccg ggcttgattt tctcgatagc caaaacgcag
     2341 gcagggaagt attgacgcaa gtggcccaag cgattggagg atgactggag aatgacaacg
     2401 gcgattctgc ggcctgacaa attttgttga tagcgaaggc ttgtatccac cgtaaccagt
     2461 acatcaaaag aaatttccgc gaggctcaaa agttcgccgt tctgcttacc agaccagccc
     2521 gattcctgaa cggtaagaca ttcgtggccg tgattgatga ggtgtctttt gagcgccctc
     2581 ggggcgcact cgtcgatcag aattctcatt tgagctgcgc gacaaggctc aaccgtgcct
     2641 cttcgatggc cgcaactgca agttcgcggc tgacgctcgg atattcttcc aggaactgat
     2701 ccaaagtgtc gcccccttcc aaatagtccg ttaagacctt gaaagggacg cgggttccgc
     2761 ggaaaacggg ttcgccgccg agaatattcg gatccttgat gatgacgctt tcagagacag
     2821 tagccatggc ggccctcctg cgcgcatccc gattatctca tccatccaca agaatcgctc
     2881 tgaaatgtgc aattcgcccg gcataaagtt gactctgaac caagtttctc ggttgtacga
     2941 gccaacgccg tcatctattc agcctttatt ccagttgatt ctgaccactc acgagtcacc
     3001 tgacctaaag ccgcggcagg acgatcccgc tctgcgactg gtacttcccc ttcttgtcct
     3061 tgtagctcac ttcacaatct tcgtcggatt cgaagaagat cacctggcac agcccttcat
     3121 tcgcgtagat ctttgccggc aacggcgtcg tgttgctgat ttccagcgtc acaaagcctt
     3181 cccactctgg ttcgaacggc gtcacattca cgatgatccc gcatcgtgcg taggttgatt
     3241 tgcccaggca aatcgtcagc acgttccgcg gaatccggaa atattccacc gaccgcgcca
     3301 gcgcaaatga attcggcgga atgatgcaca cgtcgcccct gaactccacg aacgatttcg
     3361 gatcgaaatg ctttggatcc acaatcgtcg agttcacgtt cgtgaaaatc cggaactcgt
     3421 ccgccacccg gatatcgtag ccgtacgacg acactccgta actgatcact ccctcgcgca
     3481 cct
//
LOCUS       DASYVO010000523         1692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_84242, whole genome shotgun sequence.
ACCESSION   DASYVO010000523 DASYVO010000000
VERSION     DASYVO010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84242"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="VGR03_18060"
     CDS             <1..1265
                     /locus_tag="VGR03_18060"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV1996244.1"
                     /translation="MADTGVQVPLSLYHLLDPEVLANPYPLYRRLRTEDPVHWDPYLH
                     AWIVTRYVDVITVLTRFSADRAPSPDFFEALGAPEVSPIAKVMVKQMLFLDPPAHTRL
                     RKLAAPAFTPARVHALRDHIQEIATKLIEDILARGTGRMDLLADFAAPLPAIVTAEML
                     GVPVEDHVKLKDWSATFAEMLGNFQHNPDRLGGVLRALENLTAYFENAIREQRKNPRD
                     GLVHALMSSEVDGDRLTDEEVAANSIVTMVGGQETTTNLIANGMLSLLRNPDQMARLR
                     IDNQIMPAAVEELLRYESPSQHTARLAPDDVVLGGKQIRKREAVIAVMAAGNRDPERF
                     PEPDRLDFNRPDNRHLAFGWAAHFCFGAPLARVEGQIAFETLLRRFPVLGLTGEKLVW
                     RENLGLRGMKSLPLSYDTSARAIGGAKP"
     gene            1262..>1692
                     /locus_tag="VGR03_18065"
     CDS             1262..>1692
                     /locus_tag="VGR03_18065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996245.1"
                     /translation="MSSGVETRSRVILPTFSPPKLRLLRFDDYEKTTEFLSANSFTTL
                     PYEDWRGIWVDNPLWPCMGKDFPIGWVLETTAGEIVGILGSVPTLYKFRGDDLVSAVG
                     RTWLVTAPYRGFALQLMDEYFSQPADLFINTTVSPDASAPF"
BASE COUNT          323 a          513 c          499 g          357 t
ORIGIN      
        1 caatggcgga tacgggggtc caagtcccgc tgagtctcta ccatctcctg gacccggagg
       61 tgcttgcgaa tccgtatcca ctctatcgcc gcctccgcac ggaggacccc gttcattggg
      121 atccctatct ccacgcctgg attgtcacgc gatatgtgga tgtgatcacc gtcctcacgc
      181 gcttttccgc cgatcgtgcg ccttcgcccg acttttttga ggctttgggt gcgccggagg
      241 taagcccaat cgccaaggtg atggtcaagc agatgctttt cctggatccg ccggcgcaca
      301 cgcgcctgcg caaactggcc gcgccggctt tcacgcccgc gcgcgtgcac gctctgcgcg
      361 atcacattca ggagatcgcg acgaagctga tcgaggacat tctcgcacgc ggtacggggc
      421 gcatggacct gctagccgac ttcgccgcgc cgcttcccgc catcgtgaca gccgaaatgc
      481 tgggcgtgcc ggtggaagat cacgtaaaac tgaaggattg gtccgccacg tttgccgaga
      541 tgctcggaaa tttccaacac aatcccgacc ggcttggcgg tgtgctgcgc gcgctcgaaa
      601 atctcaccgc ttatttcgaa aacgccatcc gcgagcagcg gaaaaacccg cgcgacggat
      661 tggttcatgc gctgatgtcc agcgaggtgg acggcgatcg cctgaccgac gaagaggtcg
      721 ccgcaaactc catcgtcacc atggttggag gccaggaaac caccaccaac ctgatcgcca
      781 acggcatgct ttcgctgttg cgcaatccgg atcaaatggc gcgcctccgg attgacaacc
      841 agatcatgcc cgcggctgtc gaggaactgc tgcgctatga aagccccagt cagcatactg
      901 cacgtttagc accggacgac gtcgtgctcg gtgggaagca aattcgcaag cgggaggcgg
      961 tgatcgcggt catggcggct ggtaaccggg accccgagcg atttcccgag ccggatcggc
     1021 tcgacttcaa tcggccggac aaccggcatc tcgcgttcgg atgggcggcc catttctgtt
     1081 tcggtgcgcc gctggcccgc gtcgaaggac aaatcgcctt cgaaacgctc ttgcgccgat
     1141 tcccggtgct tggactcacc ggcgagaagc tcgtctggcg cgaaaacctg ggtttgcgcg
     1201 gtatgaaatc ccttccgctt tcatacgata ccagcgcccg tgcaattggt ggagcgaagc
     1261 cgtgagttcg ggtgttgaga cgcgctctcg tgtgatattg ccgacgtttt cccctccgaa
     1321 gctacgcctt ctgcgctttg acgattacga gaaaacgacc gagttcttgt cggcgaactc
     1381 tttcacgacc cttccgtacg aggactggcg cggaatatgg gtcgataacc cgctgtggcc
     1441 ttgtatgggc aaagattttc cgatcggctg ggtgcttgag acgactgccg gggaaatcgt
     1501 tggaatttta gggtcggtgc caacactgta caaattccgc ggggacgatc tcgtctcagc
     1561 cgttggacgc acttggctcg taaccgcgcc ataccgtgga tttgcgcttc agctgatgga
     1621 cgagtatttt agtcagccag ccgatttgtt tatcaacacg acggtcagcc cggacgcaag
     1681 tgctccgttt ag
//
LOCUS       DASYVO010000524        12946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_84284, whole genome shotgun sequence.
ACCESSION   DASYVO010000524 DASYVO010000000
VERSION     DASYVO010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 12946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12946
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84284"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VGR03_18070"
     CDS             complement(<1..903)
                     /locus_tag="VGR03_18070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996246.1"
                     /translation="MVCIFLLDLVMRFVPLDSFTFRATEAVLRRYPNAEGPFPPNKHY
                     HRDNSYGVVAAVGNLPALRHYHPADFTSDAYGYRNPPALAHSNPIGIVIGDSFVIGSE
                     LPEDQTLSAQLTRLSGGYFYNAGALQPLRLGSLQSIAQRLGLHRGIVVCEFLESHATQ
                     KPPATTPYGGRGWAQSLILRAGGPSWVDRLGLPLNQLHDSPLQGLSIKLEKYLQNGDL
                     LPNSFAGSVILGKLRNGDSIAFLPGEFRTPADAKEAAGQWAAYFSWYSAELHKDGLDL
                     IVLLVPNRSTIYAPLLAQPIAVSAS"
     gene            complement(1009..2400)
                     /locus_tag="VGR03_18075"
     CDS             complement(1009..2400)
                     /locus_tag="VGR03_18075"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEV1996247.1"
                     /translation="MLFNSLGFLFLFLPIVYFGFWRLRTKNQRYIWLTIAGYIFYGTW
                     NYKFCALMAFSTAVSYFAGLGLLVTTNPRRRKLFLILPVTADLLLLGFFKYFNFTLIS
                     FAAVASWFHFPFHPPALSIILPVGISFYTFHTISYMVDAYRGVITPTRNFWEFSCYVS
                     LFSQLVAGPIVRFRQVEADLDDLDRASRTRWLDIGWSFFAIGMMKKVLIADTIAAVIA
                     PAWKDAAHFSTATAWLCVLGYTYQIYFDFSGYSDMAVGLGYLFGIRLPQNFNSPYKAL
                     NIADFWRRWHISLSSCLRDYLYIPLGGSRGSAPWLAYRNLLITMLLGGLWHGANWTFV
                     FWGGYHGVLLCLYRLFREEWDGLPASLQRTATFFLVLLGWVVFRADNFGVAAIVLTRM
                     FSWSSGFALPGLVGLLSALVIAGPIAHFAPNTFEMKHEWRPWAVAAIVIGYAAALFSI
                     AGGQPSPFLYFQF"
     gene            complement(2410..3072)
                     /locus_tag="VGR03_18080"
     CDS             complement(2410..3072)
                     /locus_tag="VGR03_18080"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV1996248.1"
                     /translation="MSRLCSSRFERAARKLGWMRIAGIDEAGRGALFGPVVAAAVILN
                     PKRRIVGLDDSKKLTAERRTELAPRIREHALAWAVAEVDAQRIDAWNIYQASRQAMTA
                     ALQQLTIQPDYLLIDAMQLDVLIEQKSLIKGDARSISIAAASILAKTHRDARMEEWDA
                     IYPQYGLARHKGYGTPDHLEALRQHGPSPLHRHSFAPVRESACWAAGAIQTSFLPDAS
                     LP"
     gene            complement(3178..5823)
                     /locus_tag="VGR03_18085"
     CDS             complement(3178..5823)
                     /locus_tag="VGR03_18085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1996249.1"
                     /translation="MERLRIFGARLRGLFRKRQLDGDLDAELRAHLEMLTEENIRRGM
                     SPVEARYTAHREFGGVEQTKELHREQRSIQFFDALLQDLRFALRGLGKRPAFAMVAIL
                     TLALGIGSTTAVFSVVDRILFRSLPYPHDDRLVSFGDKAPFEANEFVLGPDYVDWRKA
                     QTPFESVTSLVPGGADCDLTEQNPVRLKCALVESTFLPTFGIQPLLGRNFRSDEDRPN
                     ATHVALISYGLWRSRFASDRTLPGRTISLDGKPTLVVGVLPAQFEMPNLGRDDILIPS
                     ALNGSTDRGPDARQIILRAFARLKPGISISQAAAAMEPLFQQSLNYVPPQFRHEVSFR
                     VRSLRDQQIQDARVASWVLLGAVLAVLLVACTNVANLLLARATSRMRELAVRTALGAT
                     RGRLVRQSLTESLLLGVLGGLTGCWFAQLMLRLFVSIAPDSIPRLEQATIDIRVLFFA
                     LGVSILSGILFGLASALRRPAPELLTGKENHATSRGTLRQVLVTVQIALSLILLAGAG
                     LLLRSLWKLQTVALGMDAKSVITAGIDLAEYRYPDSAKQLAFFNQVEARLKQMPGITL
                     LAFSDTLPPSGGMQATFLYSIEIPGHARFSEGTGGMIGYRYVTPGYFPALGILILRGP
                     GFREEDRSPTERPVILSEALAKRLFPNGEDPLGKPFRFASQSTWRTIVGIAGDVKNNG
                     LAAPADPEFYLPWKNETDGYFRSAHIVIQSAINTQAIAKWIRSETAAIDPTVSVTIES
                     MKTRVGKLAQRPRFTAVLLSLFAGMGVLLAGIGIYGVVGFLVAQQTREIGIRIALGAT
                     PRNVLRMVLSNMVRWTVAGATLGLLGAWLCSRLLESLLFEVRAHDPYLLGLALFVLLA
                     VAFLAAWIPARRAMRVDPMVALRYE"
     gene            complement(5825..6169)
                     /locus_tag="VGR03_18090"
     CDS             complement(5825..6169)
                     /locus_tag="VGR03_18090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1996250.1"
                     /translation="MAPPRGEVLQGTLDLIVLQTLHAMGPLHGYGIAQRVKQVSEDLL
                     KLNQGTLYPALLRLEQRGWISSKWGASENNRKAKFYSLTRAGRKQLLAETESWERMTA
                     LMARLLGGTEGA"
     gene            6370..8415
                     /locus_tag="VGR03_18095"
     CDS             6370..8415
                     /locus_tag="VGR03_18095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV1996251.1"
                     /translation="MLTDQPTRRIPSSRFFSCVLLVLALLASRPALAQTRHIELNDYA
                     KITSVSDPQISPEGKTIVFVVSRPNLEQDRSDRQLVLMEIARGAQRVLTYERRGVGSP
                     RWSPRGDRLAFVAADGAGKEAKPQVFILSMAGGEARKITDAPNGIEQFAWRPNGQEIA
                     YVTSDEPENKKEIEKHHDAFEVGDNDFLATEAALPSNIWILPAEGGKAKQLTSGAWSL
                     PKSAPPSSPASPISWSPDGKWLMFTRQEHPHQGDSDLTTLQVLNVETGEIRKLTKHEK
                     LESFGVFSPDGSRVAYWYPRDGDPNNENEIYVTQTTGGDGSDAARSIDRDLLRVIWMP
                     GGDSLLVGGHDGTQTSLWLQPLAGAAKKLPLGDISPSWSFWVDAFVGRKGEIAFTGST
                     PAQPSELYYMASASDAPRRLTDFNHEIASLALGKAEKFEWKGPDGFQEDGVLFYPPDF
                     QKDRKYPLVLIIHGGPQASTTTQFSFLSQLMVTHGYVVFSPNYRGSDNLGNTYQRAIW
                     NDAGDGPGRDVISGIDALKKLSFIDASKIGVTGWSYGGYMTSWLIGHYQIWKAAMAGA
                     PVTDNYDEYNLSDGNVSVRYGFSGSPYVGDNLKAYREQSPITYAAQMKTPTLIMHDTG
                     DARVTITQAYSLFHALKDNGVPVKFLAFPVQGHFPGDPVRQMDVYRHWVEWMDQYLK"
     gene            complement(8518..8922)
                     /gene="rplS"
                     /locus_tag="VGR03_18100"
     CDS             complement(8518..8922)
                     /gene="rplS"
                     /locus_tag="VGR03_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV1996252.1"
                     /translation="MNPVIRKLHEAQLRKDLPEFRAGDTVRVNVRLQEGEGEKVKERL
                     QAFEGVVISKKGRASSATFTVRRVSFGVGIERIFPLHSPTISSIEVVGKGKVRRARLY
                     YLRDLKGKAARIKRAAREEVAAVATAGTEAKA"
     gene            complement(9081..10124)
                     /locus_tag="VGR03_18105"
     CDS             complement(9081..10124)
                     /locus_tag="VGR03_18105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV1996253.1"
                     /translation="MRYGYWLPVFGGWLRNVENENMEVSWAYVKNLAQRSEQIGFDLT
                     LIAELNLNDIKGVEAPSLDAWSTAAALAAVTERIELMVAVRPTFHLPALLAKQAANID
                     RISNGRLTLNVVSSWWADEARKYGVTFEQHDDRYARTSEWLDVLDGVWKQDHFSFSGK
                     YYRVEDNVLEPKPVAKPRPTLYAGGESPAAKELIAEKCDAYLMHGDSPERVGEKITDL
                     RERRARHNLPLLKFGVAGYAVVRETEREAQDELRRITDVQQSAAGYANYQQWLAGTQL
                     EQRVSLEDYSVSNRGLRSGLIGTPEQVAERLAQFEAAGVDLVLLQFSPQLEEMERFAE
                     SVIRRDSSLAAPA"
     gene            complement(10121..11170)
                     /locus_tag="VGR03_18110"
     CDS             complement(10121..11170)
                     /locus_tag="VGR03_18110"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996254.1"
                     /translation="MNNEIHGDGVGGNGIGLEKPIAIYYEHPDWFRPLFQQMDERGVP
                     WLKIDARHHNYDVASPEREYSLLFNRMSPSAWQRGLGHGIFYTLNYLAHLEGRGVRVV
                     NGYRCFNHEISKALQLTNLEKLGLPYPKARVINHASQALEAAEAIGYPVVIKPNIGGS
                     GAGIERFNSQNDLRGAVEANRLCFGIDSTALVQEAFTARDGIITRVEVLGGKFLYAIQ
                     IHITGESYNLCPADICQNTRGEELTRIACPVDAPKSGMKVEGYQPPRQVIEDVERIVD
                     LAGVEVGGIEYVIDDRTGRQLYYDVNALSNFVADPVRVIGFNPYARLADFLIAEAQRH
                     EANRSERVAASGGPR"
     gene            complement(11281..12066)
                     /gene="trmD"
                     /locus_tag="VGR03_18115"
     CDS             complement(11281..12066)
                     /gene="trmD"
                     /locus_tag="VGR03_18115"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:NF013874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV1996255.1"
                     /translation="MRFDLITIFPEFFAGPLSYGILRRARETGLVEVNVQDLRVFTKD
                     RHRTVDDRPFGGGEGMVLKPEPLFEAVESLLGHGVGDASNPCAADPKTAIVLMSAAGE
                     LFRQETARRSAQLERIIFICGRYEGVDERVAEHLATEEISIGDFVLSGGELPAMMVVD
                     AVTRLLPGALGNEASSQNESFSAAAQEESKTLHLVDSASHQSRITSHALLDFPHYTRP
                     AEYRGWSVPDVLIGGNHAEVANWRRAAALEKTLRNRPDLLGRE"
     gene            12323..>12946
                     /locus_tag="VGR03_18120"
     CDS             12323..>12946
                     /locus_tag="VGR03_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEV1996256.1"
                     /translation="MPFAARVTRFSLLLSLTVALLCLAGCGGYGSGGNGGGGGGGGTS
                     VPAAPTGLTPTPGDKQVALSWTAVAGATGYYVKRSTTAGGPYTQLPGTGATNFTDTGL
                     TNGTKYYYVVSAYNSYGQSANSTEVNATPSSTAAVAVTVDVLSNRHAINPNVYGGSYP
                     KDAATVTDSGMTVVRWGGNATSRYNWKTFSYNAANDYYYSDYATSEIG"
BASE COUNT         2783 a         3794 c         3860 g         2509 t
ORIGIN      
        1 ggaagcggag acggctatcg gttgagccag gagcggagca tagatcgtgc tgcggttggg
       61 aaccaggaga acgatcaggt cgaggccatc cttgtgcaat tctgcggagt accaggagaa
      121 gtaggcggcc cactggccag cggcttcctt ggcgtccgct ggcgtccgga attcgccggg
      181 caagaatgcg atggagtctc cattgcgaag ctttccaaga atgacggaac ccgcgaacga
      241 gtttgggagc agatcgccgt tctgaaggta tttttccagt ttgatggaga ggccctgaag
      301 cggcgagtcg tgtaactgat tcagcggaag tcccaggcgg tctacccagg acggaccgcc
      361 cgcgcgaagg ataagggatt gcgcccaccc acggccgccg taaggagtgg tcgcgggtgg
      421 tttttgtgtg gcgtgagatt ccaggaactc acagacgacg atgccgcggt ggagacccaa
      481 gcgctgagcg atggattgca ggcttccgag gcgaagaggt tgcagtgcgc cagcattata
      541 gaaatatcca ccgctgaggc gcgtgagctg tgccgagagc gtctggtctt cggggagctc
      601 gctgccgatc acgaaggaat cgccgatcac gatgccgata gggttcgaat gtgcaagggc
      661 gggaggattg cggtagccgt aggcatcact agtgaaatcg gcaggatggt agtggcgaag
      721 ggcagggaga tttccaacgg ccgccacgac cccgtaagaa ttatcgcggt gatagtgctt
      781 gttgggcggg aaagggcctt cggcgttcgg gtagcggcgg agcacggcct cggtggcgcg
      841 aaacgtgaag gaatcgagcg gaacgaaacg catcacgaga tcgaggagaa agatgcacac
      901 catcagccag ggaaggactc ggcctaagct cccgggtcgg accgagttag ttatcgagct
      961 gtcttggctc tgcggcactg atttcccctt catttgctcc gcttcttgtc agaactgaaa
     1021 ataaagaaac ggtgatggct gtcctcccgc gatggagaac agcgcggctg catagcctat
     1081 gacgatcgcc gctaccgccc aaggtctcca ttcgtgtttc atttcgaaag tatttggcgc
     1141 gaagtgcgcg atcggtccgg cgataacaag tgctgagagc agccccacca gcccgggcaa
     1201 tgcaaaaccg ctggaccacg agaacattcg cgtaagcacg atggctgcaa cgccaaaatt
     1261 gtctgctcgg aagacgaccc atcccagcaa gacaagaaag aatgtcgcag tgcgctggag
     1321 cgaagcgggc aagccgtccc attcttccct gaacaggcga tagagacaga gcaagacccc
     1381 gtgatagccg ccccagaaga cgaaggtcca attggcgcca tgccagagac ccccaagcag
     1441 catggtgatc aggagattgc ggtacgcgag ccaaggcgcg gaaccgcggc tccccccaag
     1501 cgggatgtag agatagtccc gcaggcagct ggagagcgaa atgtgccagc gccgccagaa
     1561 atcggcgatg ttcagggctt tgtagggcga attgaaattc tgcggcaaac gaatgccgaa
     1621 aagatacccg agtccgacgg ccatgtcgct atagccagag aaatcgaagt aaatttgata
     1681 ggtgtaacca aggacgcaga gccatgccgt tgccgtggaa aaatgcgcgg cgtctttcca
     1741 ggccggtgcg atgacggccg cgatggtatc ggcgatgaga acttttttca tcatgccgat
     1801 ggcgaagaag gaccagccga tatcaagcca cctcgttcgg ctggcgcgat cgagatcatc
     1861 gaggtccgct tccacctggc ggaagcggac aatggggcca gcgacgagtt gcgagaaaag
     1921 ggagacgtaa caagaaaatt cccagaaatt ccgggtgggc gttatgacac ctcggtaggc
     1981 atcgaccatg tagctgatgg tatggaaggt gtagaaggag atgccaacgg ggagaatgat
     2041 ggagagcgcc ggtggatgaa atggaaagtg aaaccaggaa gcgactgcgg cgaaactgat
     2101 gagagtgaaa ttgaagtatt tgaagaagcc aaggagcagg aggtcggcgg tgacaggaag
     2161 gattagaaac agtttgcggc ggcgcggatt ggttgtgacg agcaagccga ggcccgcgaa
     2221 ataacttacg gcagtggaaa aagccatgag tgcgcagaat ttgtaattcc aagtgccgta
     2281 gaaaatgtag ccggcaatgg tgagccagat gtagcgctga ttctttgtgc gcagacgcca
     2341 gaaaccgaaa taaacgatcg ggagaaacag gaatagaaaa cctaggctgt tgaagagcat
     2401 cggtcggttt cagggcaaag acgcgtctgg gagaaaagaa gtttgaatcg cgccggcggc
     2461 ccagcaggcg gactcgcgca caggcgcgaa ggaatggcga tgaagcggcg acgggccatg
     2521 ctggcgcaag gcttcgagat ggtcgggcgt gccgtagccc ttgtggcgcg ccaggccgta
     2581 ttgcggatag atggcgtccc actcttccat gcgcgcgtcc cgatgcgttt tcgccaggat
     2641 cgaggcggcg gcgatcgaga tgctgcgggc atcgccttta atcaaggatt tctgctcgat
     2701 caaaacgtct agctgcatcg cgtcgatgag caagtaatcg ggctgaatcg tcagttgttg
     2761 cagcgcggcg gtcatcgcct ggcgcgaggc ttggtaaatg ttccaggcgt cgatacgctg
     2821 cgcgtccact tcggcgacgg cccacgcgag ggcgtgttcg cggatgcgcg gcgcaagctc
     2881 cgtccggcgt tcggcagtga gctttttcga atcgtccagg ccgacaatgc gccgcttggg
     2941 attgagaatc actgcggcgg caacgaccgg gccaaacaac gcgccgcgtc cggcctcgtc
     3001 aatgccggcg atgcgcatcc agcccagttt gcgcgccgct cgctcgaaac gggaggagca
     3061 gagtcggctc attcgttagg tgtgaccccg gtgcagcagc ggggcaactc gtggcgattc
     3121 tagcacgccg agtgactggc aagagcagag gatagaatca agtgcttctt tttcgttcta
     3181 ctcatatcga agggcgacca tggggtcgac gcgcatggct cgtcgcgctg gaatccaggc
     3241 cgcaagaaat gccaccgcca gcaaaacaaa caaagccaaa ccgagaaggt acgggtcgtg
     3301 cgcacggacc tcgaaaagta gagattccag gagacgcgaa caaagccatg ctcccagaag
     3361 cccgagcgtt gccccagcaa ctgtccagcg caccatgttc gaaagtacca ttctcaaaac
     3421 attccggggg gtagcgccca gtgcgattcg aatgccgatt tcccgggttt gctgcgcgac
     3481 caggaacccg acgactccgt agatcccgat ccccgcaagt agaacgccca tgcctgcaaa
     3541 caaagaaagc aggaccgcag tgaatcgagg ccgctgcgcc agctttccta ctcgcgtctt
     3601 catgctctcg atcgtcacag aaacggttgg atcgatggcc gcggtttccg aacgaatcca
     3661 cttagcgatc gcctgtgtat tgatcgcgct ctgaatgact atgtgcgcgg agcggaagta
     3721 gccgtcagtc tcatttttcc agggaaggta aaactcggga tcggcaggcg cggcgaggcc
     3781 attgttcttt acgtctccgg caatgccaac aatggtgcgc caagtgctct gtgaggcgaa
     3841 acgaaagggt tttcctagtg ggtcttctcc attcggaaag agtctcttcg cgagtgcttc
     3901 actaaggatc acaggtcttt ccgtgggcga gcgatcctcc tcgcgaaagc cggggccgcg
     3961 caggataagt atgcccagcg caggaaaata gccaggagtg acgtaacgat agccaatcat
     4021 tccgccggtg ccctcgctga accgcgcgtg gccgggaatc tcgatggagt aaagaaaagt
     4081 agcttgcatt ccgccggaag ggggcagcgt gtcgctgaag gccagcaaag tgatgcccgg
     4141 catttgtttc agccgcgctt cgacttgatt gaagaaggct agctgtttcg cggagtcagg
     4201 atagcgatat tcggcgagat cgattcctgc ggtgatgaca cttttcgcgt ccatgccaag
     4261 tgcaacagtt tggagcttcc agaggctgcg cagaagcaga ccagctccgg caagaagaat
     4321 cagcgaaagg gcgatctgca cggtcacgag tacctggcga agcgtgccgc gcgacgttgc
     4381 atggttttct ttgccggtga gtaattcggg tgcggggcga cgcagcgctg aagccaggcc
     4441 gaagagaatg cctgaaagga ttgatactcc gagcgcaaaa aacagcacgc gaatgtcgat
     4501 cgtggcctgt tccagtcgcg gaatactgtc tggtgcgatg gaaacgaaaa gacgaagcat
     4561 caattgggcg aaccagcacc ccgtgaggcc gcccagcact ccgagcagca ggctttccgt
     4621 aagcgattgg cgaaccaggc gtccacgcgt cgcaccaagg gcggtgcgga cggccaactc
     4681 ccgcatgcgg ctggtagccc gcgcaaggag caggtttgcg acgttcgtac acgcgacaag
     4741 caacaccgcc agaaccgcgc ccagaagaac ccacgaggcg actcgggcat cctgtatctg
     4801 ctgatcgcgg agggagcgta cacggaacga cacctcgtga cgaaactgtg gcggaacgta
     4861 attaagggac tgctggaaga gcggctccat ggcggccgct gcttgcgaaa tggagatgcc
     4921 gggcttgagc cgcgcaaaag ctctcaagat gatctgccgg gcatccggcc cgcgatccgt
     4981 ggaaccattg agagcggacg gaataagaat gtcgtcgcgg ccgaggttgg gcatttcgaa
     5041 ttgtgccggg agaacgccga caaccagagt aggcttgcca tcgagtgaaa tcgtgcgccc
     5101 gggaagagtg cgatcactgg cgaaccggga acgccatagt ccgtaggaaa tgagcgcgac
     5161 gtgcgtagcg ttaggccggt cttcgtcgct cctgaaattc cggccgagga gaggttgaat
     5221 gccaaacgtt ggcaggaatg tcgattcgac cagcgcgcac ttcaatcgga cggggttctg
     5281 ctcggtgaga tcgcaatccg ccccgccggg gacaagtgat gttacggact caaacggagt
     5341 ctgcgctttg cgccagtcga cgtaatccgg tccgagcacg aactcattcg cttcgaaggg
     5401 cgccttatca ccaaagctga ccaggcgatc gtcatgggga taaggaaggc tgcggaatag
     5461 aatgcggtcg acgacgctga aaaccgccgt tgtagacccg atccccagcg caagagtgag
     5521 gatcgcaacc atcgcgaacg ccgggcgttt ccccagccct cgcagagcaa atcgcaggtc
     5581 ttggaggagc gcgtcgaaaa actggatgct gcgctgttcg cggtgcagct ctttggtttg
     5641 ttcgacgccg ccgaattcgc ggtgcgcggt gtagcgtgct tccaccgggc tcatgccgcg
     5701 gcgaatgttt tcctcggtga gcatctccag atgcgcgcga agttcggcgt cgaggtctcc
     5761 gtccagttgc cgcttgcgga ataggccgcg caaacgcgcg ccgaaaatgc ggagccgctc
     5821 catatcaggc tccttccgta ccgccgagca ggcgtgccat cagcgcggtc atgcgctccc
     5881 aactttcggt ctctgccaat aactgtttgc gcccggcgcg cgtaagcgag taaaacttcg
     5941 ccttgcgatt attttccgaa gcgccccatt tggaggagat ccagccgcgc tgctcaaggc
     6001 gcagcaacgc aggatagagc gtgccttggt tcagcttgag cagatcctcg gaaacttgct
     6061 tgacgcgctg ggcgatccca tagccgtgca gcgggcccat ggcatgaagt gtttgcagaa
     6121 cgatgaggtc gagcgtgcct tgaagaactt ccccgcgtgg aggagccatg ttttctcctg
     6181 tggacaaaca acaggagcat atactccgtc ctgtcgaatg tcaacaagaa gactcctcgg
     6241 ctgaacggca ttccccggta ttcccattcc gattgacgcg ccttctcagg gcgtggcagc
     6301 atacgcttgc aaaaagcagt tcaccctgtg gaaatctgag gatattcctt ttcaaggagc
     6361 gcaccaccga tgctcacgga tcaacccacg cgccggattc cttcttctcg tttcttcagt
     6421 tgcgttctat tggtcctggc tctcctcgcg agccggccgg ctctcgcgca aacgcgccac
     6481 atcgaattga acgactacgc gaaaatcact tccgtttccg atccgcagat ctcccccgaa
     6541 ggcaagacca tcgttttcgt cgtctcgcgg ccgaatctcg agcaggaccg cagcgatcgc
     6601 caactcgttc tgatggaaat cgccagggga gcgcagcgtg tcctgaccta cgaacgcagg
     6661 ggcgtcggct cgccgcgctg gtcaccgaga ggtgaccgcc tggcgtttgt cgccgcggat
     6721 ggagcgggaa aagaagcgaa gccgcaggtt ttcattctct ccatggccgg cggcgaagcc
     6781 cgcaagatta ccgacgcgcc gaatggcatt gagcagtttg cctggcggcc caacgggcaa
     6841 gagatcgcct acgtcacttc cgacgagccg gagaacaaga aggaaatcga aaaacatcat
     6901 gatgccttcg aggtcggcga caatgatttc ctggcgaccg aagcggcgct tccctctaac
     6961 atctggattc ttccggcgga aggcggaaaa gcgaagcagc tgacctcggg cgcttggagc
     7021 cttcccaaga gcgcgccgcc cagctcgccg gcatctccca tctcctggtc ccccgacggc
     7081 aagtggctca tgtttacgcg gcaggagcat ccgcaccagg gcgacagcga cttgacgacg
     7141 ctgcaagtgc tcaacgtcga aaccggtgag attcgcaaac tgacgaaaca cgagaagctc
     7201 gaaagctttg gcgtgttttc tcccgatggt tcccgcgtgg cttactggta tccgcgcgat
     7261 ggcgatccca acaacgagaa cgagatttat gtgacccaga caacgggggg cgacggctcg
     7321 gacgccgcac gatccatcga ccgcgactta ctgcgcgtga tctggatgcc cggcggcgat
     7381 tcgctgctgg tcggcggcca cgacggtacg caaacgtcgc tatggttgca gccactcgct
     7441 ggcgccgcta aaaaactccc gcttggcgat atcagcccct cgtggtcgtt ctgggtggat
     7501 gccttcgtcg gccgcaaagg cgaaatcgcc ttcacaggca gcacgccagc gcagccttcg
     7561 gagctgtact atatggcttc cgccagcgat gcgccgagac ggcttactga cttcaatcat
     7621 gaaatcgcct cgctggcgct tggcaaagct gaaaaattcg agtggaaagg gcctgacggt
     7681 ttccaggaag acggcgtgct cttctatccg cccgatttcc agaaagaccg caaatatccg
     7741 ctggtgctga ttattcatgg tggtccccag gcttccacca ccacgcagtt cagtttcctc
     7801 tcgcagctca tggtcacgca cgggtacgta gtgttctcac cgaattaccg tggcagcgac
     7861 aatctgggca acacctacca acgcgccatc tggaacgatg ccggcgacgg cccggggcgc
     7921 gatgtcatct cagggattga tgcgctcaag aaactaagct tcatcgacgc gagcaagatt
     7981 ggcgtgaccg gctggtccta cggcggatac atgacgtcct ggctcatcgg ccactatcaa
     8041 atctggaagg cggcgatggc cggcgcgccc gtgacggaca actacgacga atacaatctc
     8101 tcggatggca acgtgtccgt ccgctacggc ttcagcggct caccctacgt gggagacaac
     8161 ttgaaggctt accgcgagca atcgcccatc acgtacgccg cgcaaatgaa aacgccgacg
     8221 ctcatcatgc acgataccgg cgacgcccgc gtgaccatca cgcaggcgta ctcgctcttc
     8281 cacgctctca aagataatgg cgtgcccgtg aagtttctcg cttttcctgt ccaggggcat
     8341 ttccccgggg accccgttcg ccagatggac gtctatcgcc actgggtgga atggatggac
     8401 caatacttga agtaggaact acttcgcttc gcgtgaactt aggcgatgca tctaaaaaga
     8461 aaaagccccg ttcacacgga gcttctttct gaatttcggg gatctgatgg aagctactta
     8521 agccttcgct tcggtgccgg cggtcgccac agcagccact tcctcgcgtg cggcgcgctt
     8581 gatgcgggcg gccttgccct tcagatcgcg caggtaatac agccgtgccc ggcgaacttt
     8641 acccttgcca acgacttcaa tcgagctgat cgtgggcgag tgcagtggga agatacgctc
     8701 gatgcccacg ccaaagctga cccggcgcac cgtaaaggtc gcgctggacg cccggccttt
     8761 cttggagatc accacgccct cgaatgcctg gaggcgttcc ttcaccttct cgccttcgcc
     8821 ttcctgcagg cggacgttga cgcgcacggt gtcgcccgcg cggaattcgg ggaggtcttt
     8881 gcgcaattgc gcttcatgta gtttacgaat taccggattc atgttcctgt ttccttgcag
     8941 acccactcag gcggggccag ctcactaaag acaatcgcta attatagaca atttatcgcc
     9001 attttcctag cggattcagg ccgctggtga agccgtccct ttcctttccc cgagtggcgc
     9061 gctggacgac aagctcgctg ctaagccggt gccgccaggg atgaatcgcg gcggatcacg
     9121 gattccgcaa agcgttccat ctcctcgagt tgcgggctga attgcagtag cacaaggtcg
     9181 acgcctgcgg cttcgaattg cgccaggcgc tccgccacct gttccggcgt gccaatcagc
     9241 ccggaccgca ggccgcgatt cgaaaccgaa taatcttcca gcgaaacccg ctgctccagt
     9301 tgcgttccgg ccagccattg ctggtaattc gcgtagcctg cggcagattg ctgaacatcc
     9361 gtgatgcgcc gcagttcatc ctgcgcttcg cgttccgttt ctcgaacgac ggcgtagccc
     9421 gcgaccccga atttgagtaa cggcaaattg tgccgtgcgc ggcgttcgcg caggtccgtg
     9481 attttctcgc cgacgcgttc cggcgaatcg ccgtgcatga gatacgcgtc gcacttctcg
     9541 gcgatcagct cttttgccgc cggagattcg ccgcccgcgt acagcgttgg ccgcggcttc
     9601 gccaccggtt tgggctcgag aacattgtct tccacacggt aatacttccc cgaaaacgaa
     9661 aaatggtcct gcttccacac gccatccagc acgtcgagcc actcgctggt gcgcgcgtag
     9721 cggtcgtcat gctgttcgaa cgtgacgcca tacttccgcg cttcgtccgc ccaccacgaa
     9781 gaaactacat tcagcgtgag ccggccattg ctgatccgat cgatgttggc cgcctgcttc
     9841 gccagcagcg ccggaagatg gaacgtcggc cgcaccgcca ccatcaattc gatccgctcg
     9901 gtcaccgcgg ccagcgccgc cgccgtggac cacgcatcca gagaaggcgc ttcgacgcct
     9961 ttgatgtcgt tgagattcag ctcggcgatg agcgtcaggt caaaaccaat ctgctcgctg
    10021 cgctgcgcga gattcttcac gtacgcccaa cttacttcca tattttcgtt ttcaacgttg
    10081 cgcagccagc cgccgaaaac gggaagccag tagccgtatc tcatcgcggg cctccgcttg
    10141 cggcaacgcg ttcgctgcga ttcgcctcgt gacgttgcgc ctccgcgatg aggaagtccg
    10201 cgagccgcgc gtaaggattg aaaccgatga cccgcaccgg atcggcgaca aaattggaaa
    10261 gcgcgttcac gtcgtaataa agctgccgcc cggtgcggtc gtcgatgacg tactcgatgc
    10321 cgccaacctc taccccggca agatccacga tgcgctccac gtcttcgatc acttgccgcg
    10381 gcggctggta gccttccact ttcattccgc tcttcggcgc gtccaccggg cacgctatgc
    10441 gcgtcaactc ttcgcctcgg gtattctggc agatatccgc aggacacaaa ttgtagctct
    10501 cgccggtgat gtgaatctgg atcgcataca gaaatttacc gccaagaacc tcgacgcgcg
    10561 taatgattcc atcgcgcgcc gtaaacgctt cctgcaccag cgccgtggaa tcgatgccaa
    10621 agcagaggcg attcgcttcc accgctccgc gcagatcgtt ctgcgaattg aagcgctcga
    10681 tccccgcgcc actgccgccg atgttcggtt tgatgaccac gggatacccg atggcctccg
    10741 cggcctcaag cgcctgcgac gcatgattga tgacccgggc tttgggatac ggcaatccca
    10801 gtttttccag attcgtgagc tgcaacgctt tcgaaatctc gtggttgaag caccggtagc
    10861 cgttgaccac gcgcacgccc cggccttcga ggtgcgcaag atagttgagc gtgtagaaga
    10921 tgccgtggcc caggccgcgc tgccacgccg aaggactcat gcggttgaag agcagagaat
    10981 attctcgctc cggcgaagcc acatcgtaat tgtggtgccg cgcatcaatc ttcagccacg
    11041 gcacgccgcg ctcatccatt tgctgaaaca gcggccggaa ccagtccgga tgctcgtagt
    11101 aaatggcgat cggtttttcc aacccgatgc catttccccc gacgccatca ccatggatct
    11161 cattgttcat aaaacctcgc ctttagcgct aatcttgcga gcgcttgcag agcatgggct
    11221 gtcttgcgga tatggagcgg ccctctcaca ggatgaatga ggacctcaag cagttccatt
    11281 ttattccctt cccaacagat cgggacgatt gcgcagggtc ttctcaagag ctgcggcgcg
    11341 ccgccagttg gcgacttccg cgtgatttcc gccgatgagg acgtcgggga cggaccatcc
    11401 gcgatattcc gctgggcgtg tgtagtgtgg aaaatctaga agtgcgtgac tcgtgatgcg
    11461 tgactggtga ctcgcgctat ccaccagatg aagcgtcttg gattcctcct gcgcggccgc
    11521 gctgaacgat tcgttttgcg atgaagcctc attccccaac gccccaggca acaagcgcgt
    11581 gaccgcgtcc accaccatca tggccggcag ttcaccgccg ctcagcacga aatctccaat
    11641 cgatatttcc tccgtcgcca aatgctccgc cacgcgttcg tcaacgccct cgtacctccc
    11701 gcaaataaaa ataattcgct ccaattgcgc ggagcgccgc gccgtttcct gccggaacag
    11761 ctctcccgcc gccgacatca gcacaattgc ggtcttcgga tcagcagcgc atgggttcga
    11821 ggcgtcgccg acgccgtgcc caagcagcga ctccaccgct tcaaacaatg gctccggctt
    11881 cagcaccatg ccctcgccgc cgccgaacgg ccggtcatcc accgtgcggt gacgatcctt
    11941 ggtgaacacc cgcaggtcct gcacgttgac ctcgaccagg ccggtttctc gcgcgcggcg
    12001 gaggatgccg taactgagcg ggccggcaaa gaattccgga aagatggtga tgagatcgaa
    12061 gcgcatgcaa aaccagtgta gccaatcgag ggaccagccg ccattctgac ttacttctct
    12121 ttcacttata tcctggttgc attggaagtc gtaagcgttt acgattctcc ggactggaac
    12181 tatttctgtc ccattggaca gcttgtcggg cctctttcgg tctcataacc tcttgctgtt
    12241 gaagcagttg ttcccagccc gaggatcgga gacgccggag actcgacgca cccgcattcg
    12301 cacttcctgg gaggccaatt ttatgccctt tgccgctcgc gtgacccgct tttccttgct
    12361 cttgtccttg accgtcgccc ttctatgcct ggctggctgc ggcggttacg ggagcggtgg
    12421 caacggcggt gggggtggtg gcggaggcac atctgtgcca gcggcaccca ctggcctcac
    12481 gccgacgccg ggagacaagc aggtagctct gtcatggact gcggtcgccg gcgctacagg
    12541 ctattacgtc aagcgctcca ccacggctgg cggaccctac acgcagttgc cagggaccgg
    12601 cgcgacgaat ttcaccgaca cgggcctgac caatggcacg aagtactact acgttgtttc
    12661 cgcctataac tcctacggcc agagcgccaa ttctacggaa gtcaatgcca cgccatcgtc
    12721 cactgccgcg gttgcggtga cggtagacgt gctgagcaat cgccacgcga tcaacccaaa
    12781 cgtttacggc ggaagctatc cgaaagacgc ggccacggtt accgatagcg gaatgaccgt
    12841 cgtgcgctgg ggcggcaacg ccacctcgcg ttacaactgg aagacgtttt cctacaacgc
    12901 ggcaaacgat tattactata gtgactacgc gaccagcgaa atcggg
//
LOCUS       DASYVO010000525         9253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_211465, whole genome shotgun sequence.
ACCESSION   DASYVO010000525 DASYVO010000000
VERSION     DASYVO010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9253
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211465"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VGR03_18125"
     CDS             complement(<1..850)
                     /locus_tag="VGR03_18125"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEV1996257.1"
                     /translation="MKKRASKPKTLSRGFRVVALGGGTGLSTLLRGLKELVIRRTDEH
                     ASTERPIADLAAIVTVTDDGGSSGRLRRENRILPPGDIRNCMVALSKDEALLGRLFQY
                     RFHAGRGLVGHNFGNLFLAALTHVTGDFSEAIRVSSKVLAIRGRIFPSTLSNVHLVAT
                     LENGRVVHGETRITASCAPIKKLKLSSRGVRALPRAIEAIKQADLILLGPGSLYTSIL
                     PNLLIPEIAGAIAKSNAPRVYIANLMTQPGETAGYALADHLRAIQKHVPRRVIDWVVA
                     NRQPVSP"
     gene            complement(968..1603)
                     /locus_tag="VGR03_18130"
     CDS             complement(968..1603)
                     /locus_tag="VGR03_18130"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1996258.1"
                     /translation="MAKARKPSSGSSRHSKGTARSQSKSQKKKTFPPPPPAQRGGVEL
                     VDPRVQAQLKVYDEALTLFHQQKFARAKQELEKVLEGPSKELADRARMHVKIAEQRMK
                     PSQEQNPHTADEHYQRGVAMMNVGRWDDARESLDKARKAAPKADHIHYALAALDCLTG
                     EADSALANLKVAIQLRPENRYHARNDEDFAFLQEDPRFTELLYPEKDGTAG"
     gene            1847..2434
                     /locus_tag="VGR03_18135"
     CDS             1847..2434
                     /locus_tag="VGR03_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEV1996259.1"
                     /translation="MPFINFPAREINCKLVYYGPGLGGKTANLQWIYDHTGQNQKGKM
                     VSLATETDRTLFFDFLPLDLGTVRGFKTRFHLYTVPGQVFYEASRKLILKGADGVVFV
                     ADSQEERLDANFETMENLMEHLKEHNLNFATIPYVLQLNKRDLPNAMSVPELTKQLQK
                     KGEPVLEGVAIQGLGVFETLKELAKLVLAELKKGG"
     gene            complement(2512..2868)
                     /locus_tag="VGR03_18140"
     CDS             complement(2512..2868)
                     /locus_tag="VGR03_18140"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV1996260.1"
                     /translation="MTDRVPMEINVRESGGVSVLEVHGRLTIGEPAEQLHEALESVVK
                     GGAKKLVVILNGIPQIDSSGISTLVRISIKLAREGGVLRLVCGQGRVRDALTVTRLVE
                     AIPTFESESAALANFA"
     gene            complement(3082..3207)
                     /locus_tag="VGR03_18145"
     CDS             complement(3082..3207)
                     /locus_tag="VGR03_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996261.1"
                     /translation="MLPTQQSLYADLKRAAADCFPRALGTFLEAPEAVGATPIAG"
     gene            complement(3223..3426)
                     /locus_tag="VGR03_18150"
     CDS             complement(3223..3426)
                     /locus_tag="VGR03_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996262.1"
                     /translation="MLVCYAFENRNTWFILAFALACVLGSLYGFLQGAWPFGLVEAIW
                     AAVAARRWWLAARSNFAVRKPTS"
     gene            complement(3523..4692)
                     /locus_tag="VGR03_18155"
     CDS             complement(3523..4692)
                     /locus_tag="VGR03_18155"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV1996263.1"
                     /translation="MHDPLPLPLALLIIFGSAKLLAELCERLGQPGIVGEILAGALVG
                     PSVLGWINPNETLKALSDLGVMFLLFGVGLEVKASELLKVGGKATLVATIGVIVPFFA
                     GWGILEAWGAPQIEAVFVGAAMVATSVGITASVLSARGVLHEVASQVILAAAVIDDVL
                     GLIVLAVVSSVARGRVNLWEIALVASLATAFTVVMAIWGTTAVKRVLPFFGSRARADE
                     AEFHIALVFLFAMALLAQYTGVAAIVGAFLAGLALSDSSDARMRTLTRGVSELLVPFF
                     LAGIGLHLNFGVFRSRSTIALALVILAAAVVTKLVGCGLGAISLGWQNVLKIGLGMVP
                     RGEVGMVVAQIGLGMAVISTEVYSVVVFMAVATTLLTPLLLKIAFRTPALVPAEA"
     gene            complement(4702..4860)
                     /locus_tag="VGR03_18160"
     CDS             complement(4702..4860)
                     /locus_tag="VGR03_18160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996264.1"
                     /translation="MNLKWSFYFGAAVMAAYLLISRGVPIVPVLAGCGLAALFTWRKM
                     SRRSAQRL"
     gene            complement(4857..5390)
                     /locus_tag="VGR03_18165"
     CDS             complement(4857..5390)
                     /locus_tag="VGR03_18165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996265.1"
                     /translation="MTATSTSMQPRPGALAPGIEFPRLPALPWRDPHNVPPEELAKVI
                     ASLTEACTHNPANADLRICLGMAHAMNYDAYRSMDALEEACGIEPQNFLARFKYAELL
                     FRLRALERAREETTRALELATSGWELNQARHQLSEIRRLLREGTQKPTWSKSLKVPLL
                     GLVLIMMVVSILFTVFR"
     gene            complement(5409..7061)
                     /locus_tag="VGR03_18170"
     CDS             complement(5409..7061)
                     /locus_tag="VGR03_18170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEV1996266.1"
                     /translation="MNTTTKRLLTFLLAALCLAPVGLAQAPAAAPAAAAAPATPAPKP
                     ITDADVANVPVPTADAHAKGDPDGSLTGNASDVTVSDSKKGLTIGDLVNQAGQNKIAI
                     NFVWTLVTGFLVMFMQAGFAIVETGLCRAKNANHTMMMNFMVYGVGMLAYWLIGFAIQ
                     EGGVGAVANLGGTAPLSAEYAVTLFGKSFGLFGQNGLFLVHKGTYDVGVMVLFLFQMV
                     FMDTALTIVTGAAAERWKYAAFVAASFLMGAFTYPIFANWAWGGGWLSTLGTNFGLGK
                     GYCDFAGSGVVHAVGGITALAASMILGPRIGKYTREGKSNAILAHDIVIVLTGCFILA
                     FGWFGFNPGSTLGASSNGNLRIGSIAVNTMLAGMAGSFGAMLYMWLRYSKPDASMSGN
                     GLLAGLVAITAPSGFVNPVGAVIIGLIAGVLVCISVEYVDRVLKVDDPVGAISVHGTN
                     GLWGVLSVGLFADGKSNYGGAWNGVTGSVTGIFYGDNGGQLVAQLIGIVTLVGFVFTL
                     SFVFNLLIDWVIGQRVSAKTELEGLDLPEMGALGYPEFELKS"
     gene            complement(7069..7251)
                     /locus_tag="VGR03_18175"
     CDS             complement(7069..7251)
                     /locus_tag="VGR03_18175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996267.1"
                     /translation="MILIGILVTLLGFVISILSLALNSVGARLVVVLLGLTVSLFGII
                     GVLNKAYLKNAIWKKG"
     gene            complement(7264..7443)
                     /locus_tag="VGR03_18180"
     CDS             complement(7264..7443)
                     /locus_tag="VGR03_18180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996268.1"
                     /translation="MAANATQAVGVSTFLLGFTTLSGAIYKGGSILLFLVTAVLLGIS
                     VATFRKCKPLENAEN"
     gene            complement(7506..8861)
                     /locus_tag="VGR03_18185"
     CDS             complement(7506..8861)
                     /locus_tag="VGR03_18185"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEV1996269.1"
                     /translation="MRISSKCCFSLLLGSALAVSAFGQQANTDQQKTAGTQVVASTMD
                     DLRERILLDRIEQLEKRLADLEGRTASAQPAPGQAAATTAPAAQEPAPATPPAKAPEA
                     PAPPTWSAGPIDFSGRVSAYYTFNGNHPASMTNQLYNFNVKANQFSLDMAEFNMSHTA
                     DPVGFELDLGFGRAFDIIAGSEPQPSIFRNIRQAYASLKPTKAKGLEIDFGKFVTSDG
                     AEVIETDANWNYSHSLLFAWAIPYYHFGLRATYPVNPHFTTGIMVANGWNNVEDNNSG
                     KLVMFMETFTYKKFTLFENIHTGPENAGTNKGWRHLYDTTLLLTPTAKFNAYINFDYG
                     HNQLPPGTLTVLGNQAAVWWGGAVALHFMPTSKWSFTPRVEYFKDRDGFAIGSGVPQD
                     LKEGTFTAEYKMMEGLLGRVEYRRDWSNQPFFDRGLNLGVHKNQDTLSLGVLAFFGPK
                     R"
     gene            9132..>9253
                     /locus_tag="VGR03_18190"
     CDS             9132..>9253
                     /locus_tag="VGR03_18190"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEV1996270.1"
                     /translation="MKKLEAIIQPSRFESVKDALLEMGVGGLTVTEVRGHGRQKG"
BASE COUNT         2040 a         2617 c         2858 g         1738 t
ORIGIN      
        1 cgggcgacac cggctggcga ttggcgacga cccaatcgat gacgcggcgc gggacgtgct
       61 tctggatcgc gcgcagatgg tcggcgaggg cgtagccggc ggtttcgcct ggctgggtca
      121 tcagattggc gatatacacg cgcggagcgt tcgatttggc gatggcgccg gcaatttccg
      181 ggatcagcag attcggaaga atgctggtgt agagcgagcc ggggccgaga agaatcaggt
      241 cagcctgctt gatggcttcg atggctctgg ggagcgcacg aacgccgcgc gaggaaagct
      301 tgagtttctt gatgggggcg cagctggcgg tgatgcgggt ttcgccgtga acgacgcggc
      361 cgttttcgag cgtggcgacg agatggacgt ttgaaagcgt ggacggaaag atgcgcccgc
      421 ggatggcgag cactttgctg ctcacgcgga tggcttcgct aaaatcgccg gtgacgtggg
      481 tgagcgcggc gaggaaaaga ttgccgaaat tgtggccgac gaggccacgg ccggcatgga
      541 aacgatattg gaacaggcgg ccgagaaggg cttcatcttt cgaaagcgcg accatgcagt
      601 tgcggatgtc gccgggcggg aggatgcggt tctcgcggcg caagcggccg gagctgccgc
      661 cgtcatcggt gacggtgacg atggcggcga gatcggcgat ggggcgctcg gtgctggcgt
      721 gttcgtcggt gcggcggatg acgagttcct tgaggccgcg aaggagcgtg gagaggccag
      781 tgccgcctcc aagggcaacg acgcggaagc cgcgggaaag agtcttcggt ttggaggcgc
      841 gttttttcat gctgacattc gggatggcag aaccggcgtc atcttacagg aaagaattta
      901 acacagagaa ctcagaggac atcgagagca cggagaagaa aaaccggaca ggctaaagcc
      961 tgtcccacta accggcggtg ccgtctttct ccggataaag cagctcggtg aagcgcgggt
     1021 cttcctggag gaaagcaaaa tcttcgtcgt tgcgcgcgtg atagcggttt tccgggcgca
     1081 actgaatggc gacctttaga ttggccaggg cagaatcggc ttcgccggtg aggcaatcga
     1141 gcgcagccag cgcgtagtgg atatggtcgg ccttgggcgc ggctttgcgg gctttgtcga
     1201 gactttcccg ggcatcatcc cagcggccga cgttcatcat agcgacgccc cgttggtaat
     1261 gctcatccgc ggtgtgggga ttttgctcct gagacggctt catgcgctgc tcggcgattt
     1321 tcacgtgcat gcgcgcgcga tccgcgagtt ccttgctggg gccttccagg actttttcaa
     1381 gttcctgctt agcgcgggcg aatttctgct gatgaaagag ggtcagggcc tcatcgtaga
     1441 ctttgagttg cgcctggacg cgcgggtcaa ccagctcgac acccccacgc tgggcgggcg
     1501 gagggggagg gaaagtcttc tttttctgtg acttagactg gctccgcgcc gtgcccttgg
     1561 aatgcctgga tgaccctgaa gagggcttac gcgcttttgc catggagcga atgctagcag
     1621 agcaggattc tggcgtcaac ggatgagcgc gtgggcattt tggcaaccag gaaatccaca
     1681 ggcgttaccg aacggaggca tggggcaaaa atagggcgtg gagttcgggg cggaatcgcg
     1741 gacactgtag gtgcagtacg cgcaaggtga cacgcggggt gggaaggctg gcctgcctgc
     1801 gccgcgtatg ctataagttt gtggccgaat tcgggttgac aacaacgtgc ctttcatcaa
     1861 ttttcctgct cgcgaaatta actgcaagct cgtgtactac ggtccaggtc tgggcggaaa
     1921 gaccgccaat ctgcagtgga tctacgacca taccggacaa aaccagaagg ggaagatggt
     1981 ttctctggcc acggagacgg accgcacgct cttcttcgac ttcctgccat tggatttggg
     2041 aacggtgcgc ggattcaaga cgcgctttca tttatatacc gtgcccggac aggtgttcta
     2101 tgaggccagc cgcaagctga tcctgaaagg cgcggacgga gtggtgttcg tggcggattc
     2161 gcaggaagaa cggctggacg cgaatttcga aaccatggaa aacctgatgg agcacctgaa
     2221 agaacacaac ctgaatttcg cgacgattcc ttacgtgctg caattgaaca agcgcgacct
     2281 gccaaatgcg atgtccgtgc cggaattgac gaagcagctg cagaaaaaag gcgagccggt
     2341 tttggagggc gtggcgattc agggattggg cgtgttcgaa acgctgaaag agctggcgaa
     2401 gctggtgctg gcggagctga aaaagggcgg gtaggcttct tcgggtaagt ttcttccccg
     2461 aattcttcca aagttaagcc agtgattttc gatgcaaacg gatacgtgtg tctacgcgaa
     2521 attggcgagg gcggcggatt cggattcgaa ggtggggatg gcttcgacga ggcgggtgac
     2581 ggtgagggcg tcgcggacgc ggccctggcc gcagacgagg cggagcacgc cgccttcgcg
     2641 ggcgagcttg atggagatgc gcaccagcgt ggagatgcca gagctgtcga tctgcgggat
     2701 gccgttcagg atgacgacga gtttcttcgc gccacctttg acaacggatt caagagcttc
     2761 gtgaagctgc tcggcaggct cgccgatggt aagccggccg tgaacttcga gcacggatac
     2821 tccgccggat tcgcggacgt tgatttccat gggcactcgg tcagtcatgc ggcgctttct
     2881 atcggatctc tcctacaaaa aagtataccc aacaacccgg cggtcgtctc ggatagcgat
     2941 gcacagcagc ctgcagggca ttatacgatg ccgggcgtat gggacaagga aatgcgcacg
     3001 ggtcctgcgc gggatgggat gctctgcggt gcgaaggcac gcggtcgcca gaatgacaaa
     3061 cggagccaca agcacacagc gtcatccggc gatgggcgtg gcacccaccg cttcgggcgc
     3121 ttcaaggaat gtgccgaggg cccgcgggaa acagtcggcg gcggcgcgtt tcaggtcggc
     3181 atagagggac tgttgggtgg ggagcaagaa atagactcag atttaactgg tcggttttct
     3241 aaccgcgaag tttgaccgcg ccgccagcca ccagcgccgc gctgccaccg ccgcccagat
     3301 agcctccacc agtccgaacg gccaagcccc ttgaagaaaa ccgtaaagcg agccgagcac
     3361 gcatgccaag gcgaacgcca ggatgaacca cgtatttcgg ttctcgaacg catagcacac
     3421 gagcatggcg gtcacggcga ataatccaaa aagtgtaagt gaactcaggc tgtttctccc
     3481 ggtggttctg tatctgacaa actcgcggaa tctggaaacc ggctaggctt cggcgggcac
     3541 caaggcggga gtccggaaag cgattttcag aagaagcggc gtgagcaggg tggtcgccac
     3601 ggccatgaac acaaccacgc tgtagacctc cgtgctaatg accgccatgc cgaggccgat
     3661 ctgcgccacc accataccca cttctccccg cggcaccata ccaagaccga tcttcaacac
     3721 gttttgccag ccgagcgaga tggcgccgag accgcagccg accagcttgg tcaccacggc
     3781 ggcggccaaa atcaccagcg ccagagcgat cgtcgaccgg gaacggaaca ctccgaaatt
     3841 taggtgcagc ccgatgcccg cgagaaaaaa gggcaccagc aactcgctca cgccccgagt
     3901 caacgtgcgc atgcgggcgt cggaactgtc cgagagcgcg aggcccgcca gaaacgcgcc
     3961 aacgatcgcg gcaacgccgg tgtactgcgc cagcagcgcc atggcgaaga ggaacaccag
     4021 cgcgatatga aactccgctt catcggcacg cgcacgcgat ccgaagaacg gcagcacgcg
     4081 cttgacggcc gtggtgcccc aaatcgccat gaccacggtg aaggccgtcg ccagcgacgc
     4141 gaccagcgcg atctcccaga gattgacgcg accgcgcgcc acactgctga ctaccgccag
     4201 cacgatcagc ccgaggacat cgtcaatcac cgcagcagcc aggatgacct gactggccac
     4261 ctcatgaagc accccacgcg ccgagagcac cgacgcggtg attcctacgc tggtggccac
     4321 catcgccgct ccgacaaaaa ccgcttcgat ttgcggcgct ccccaggctt ccagaattcc
     4381 ccacccagcg aaaaatggca cgatcacgcc tatagtggcc acgagagtgg ccttcccgcc
     4441 caccttcaag agttcggaag ccttgacctc aaggccaacg ccaaagagca ggaacatcac
     4501 gcccagatcc gagagggcct ttagtgtttc gttgggattg atccacccga ggacgctggg
     4561 gcctaccagc gcgccggcca ggatttcgcc gacgatgcca ggctggccca gccgttcgca
     4621 caactccgcc agaagcttgg cggaaccgaa aataatcagc agggccagcg gcagaggtag
     4681 cggatcgtgc attatgcggc cttagaggcg ctgcgcggat cggcgagaca tcttgcgcca
     4741 ggtgaacagc gcggccaagc cacagcccgc cagaacgggc acaatgggga cgcccctgga
     4801 gatcagcaag taagccgcca tcacggcggc cccaaaataa aagctccatt tcaagttcat
     4861 cggaacaccg tgaaaagaat gctgacgacc atcataatca aaaccaatcc aagcagggga
     4921 actttcagcg acttcgacca ggtgggcttt tgtgtcccct cgcggaggag cctgcggatt
     4981 tcggaaagct gatggcgtgc ctggttgagt tcccagccgc tggttgccag ttcgagcgcc
     5041 cgggtggttt cctcccgtgc ccgttccaga gcgcgcaggc gaaagagcag ttcggcatac
     5101 ttgaatcgcg ccaaaaagtt ttgcggctcg atgccgcaag cctcttcgag agcgtccatc
     5161 gagcgatagg cgtcgtagtt catcgcatgg gccatgccca ggcagatgcg caggtcggcg
     5221 ttggcgggat tgtgggtgca ggcctcggtc agcgaggcaa tgaccttcgc cagctcttcc
     5281 ggcggaacat tatgcggatc ccgccaggga agcgcgggga gacggggaaa ctcgatgccg
     5341 ggagccagag ctcccggccg aggttgcatt gatgtgctag tggcagtcat ccgaagccct
     5401 cctcaagtct atgacttcag ctcgaactcg ggatagccca gtgctcccat ttcaggaagg
     5461 tccaagccct cgagttccgt cttagccgag acgcgctggc caatgaccca atcgatcagc
     5521 aggttgaaga caaaactgag agtgaagacg aaaccgacca gggtgacaat gccgatcaac
     5581 tgggccacga gttggccacc attgtcgcca taaaagatgc ctgtgaccga gccggttacg
     5641 ccgttccatg cgccgccgta gttgctcttt ccgtcggcga aaaggccgac ggaaaggaca
     5701 ccccagaggc cgttggtgcc gtgtacggaa atggccccga ctggatcgtc tactttaaga
     5761 acacggtcta cgtactcgac cgaaatgcaa acgaggacgc cagcgatgag gccaatgatc
     5821 acggccccaa ccggattgac gaaacccgaa ggcgccgtaa tggccaccag cccggctaga
     5881 aggccgttgc cgctcatcga agcgtccggt ttactatagc ggagccacat atagagcatc
     5941 gcgccgaacg agccggccat acccgcgagc atggtgttaa ccgcgatcga gccgatgcgc
     6001 agattgccat tgctcgaagc gcccagcgtc gagccggggt tgaatccgaa ccagccgaag
     6061 gccaggatga agcagccggt caggacaatc acgatatcgt gcgccaggat cgcgttggac
     6121 ttcccttccc gggtgtactt gccgatgcgc ggcccgagga tcatagacgc ggcaagggcg
     6181 gtgatgccgc cgaccgcgtg taccacgccc gacccggcaa agtcacagta gcccttgccc
     6241 aggccgaagt tcgtcccgag ggtggaaagc cagcctccgc cccatgccca attggcaaag
     6301 atgggatagg taaacgcgcc catgaggaac gaagcggcga cgaaggccgc atacttccaa
     6361 cgctcggcgg ctgcgccggt gacgatcgtc aatgcggtgt ccatgaatac catttggaac
     6421 aggaagagca ccatgacgcc gacgtcgtaa gttcccttgt gaactagaaa cagtccgttc
     6481 tggccaaaaa gcccaaagga cttgccgaac aacgtgacgg cgtattccgc gctcagggga
     6541 gcggtcccac caaggttggc gacggcgccg acgccaccct cctgtatggc gaagccaatg
     6601 agccagtaag ccagcatgcc gacgccataa accatgaagt tcatcatcat ggtgtggttt
     6661 gcgttcttag cgcggcacag gcccgtctcg acgatggcaa accccgcttg catgaacatg
     6721 accaagaagc cggtgacgag ggtccaaaca aagttgatgg caatcttgtt ttgcccggcc
     6781 tggttcacca aatctccgat ggtgagccct ttcttgctgt cgctcacggt gacatcactg
     6841 gcattgcctg tgagcgagcc atcaggatca cccttggcat gggcatccgc agtcggcacg
     6901 ggcacattcg ccacatcagc atcggtgatg ggcttcggag cgggcgtcgc cggagcagcc
     6961 gcggcggccg gggcggcagc gggcgcctgg gcaagaccca ccggcgccag acagagcgcc
     7021 gcaagcagga acgtcagcaa tctcttcgtc gtcgtattca tcctcgcctc accccttctt
     7081 ccagattgcg ttcttgagat aggccttgtt aagcacgccg atgattccga acaagctgac
     7141 cgtcaaaccc agaaggacga ccaccagccg ggcgccgacg gaattcagag ccagactgag
     7201 gatactgatt acaaacccaa gcagagttac aagtattccg ataagaatca tgtctcgctc
     7261 tcctcaattc tccgcgtttt ccagcggctt acactttcga aaggtggcca ccgagatccc
     7321 caagagcacc gccgtcacca ggaagagcaa aatgctcccc cctttataga tggccccgga
     7381 aagcgtggtg aaccccagca aaaaggtaga taccccaaca gcctgtgtgg catttgctgc
     7441 catcgaaaac ctcccctcta aagttcgatt cagtcttcta caacaacttc gattcgaaaa
     7501 aatacctacc gcttgggccc aaagaatgcc agaactccca acgacagtgt gtcttggttc
     7561 ttgtggaccc cgagattcag tccgcggtca aagaatggct ggttggacca gtcacgacgg
     7621 tattcgacgc gaccaagcaa accttccatc atcttgtact cagcagtgaa ggtcccctct
     7681 ttgaggtctt gtggcacgcc ggaaccgatg gcaaacccgt cgcggtcctt gaaatactcc
     7741 acacgaggtg taaacgacca cttggaagtt ggcatgaagt gcagcgcgac agcccctccc
     7801 caccacactg ccgcctggtt gcccaaaaca gtcaacgtgc caggggggag ttggttgtgg
     7861 ccgtagtcaa agttgatgta cgcgttgaat ttggccgtcg gggtcagcag cagagtcgta
     7921 tcgtagaggt gccgccagcc cttgttcgtg cccgcgtttt ccggaccggt gtgaatattt
     7981 tcaaacaaag tgaatttctt gtaagtgaac gtttccatga acatcacgag cttgccgcta
     8041 ttgttgtcct cgacgttatt ccatccgttg gccaccataa tccccgtcgt aaaatgcggg
     8101 tttacgggat aggtcgcacg gagaccgaag tggtagtagg ggatggccca ggcaaacaac
     8161 agcgagtgcg agtagttcca gttggcgtca gtctcaatca cttcggcacc gtcgctggtg
     8221 acgaattttc cgaaatctat ttccagccct ttggccttgg tgggcttcaa gcttgcatag
     8281 gcctgccgga tattgcggaa gatcgagggt tgcggctcgc ttccggcgat aatgtcgaaa
     8341 gcgcgcccaa aacccaagtc gagttcaaaa ccaactggat ctgcggtgtg cgacatgttg
     8401 aactccgcca tgtccaggct aaactggttg gccttcacgt tgaagttgta gagttgattg
     8461 gtcatgctcg ccggatggtt gccgttaaac gtgtagtaag cagaaacgcg accgctgaaa
     8521 tcaatgggac cggcggacca agtgggcggt gcgggagctt ctggcgcttt cgcaggcggc
     8581 gttgccgggg caggttcctg agccgccggg gcggtggtgg ccgccgcttg gcccggggca
     8641 ggttgtgcgg aagctgtgcg accctcaagg tcagcaagac gcttttccag ttgctcgatg
     8701 cgatcgagca ggattcgttc gcgcaggtca tccatcgtgc tggcgacgac ttgcgtaccc
     8761 gcagtctttt gctggtcggt atttgcctgc tgtccgaaag cggataccgc cagcgccgaa
     8821 ccaagtaata agctgaaaca acacttcgag gaaatgcgca ctttatattc tccttgggtc
     8881 ttccctggat cccctgggcc ctgcgactct ggtcaaatac gaaagaggac accgctcgtc
     8941 gcttgagggc cgactgtagc gatgactcat tgggaaagcc aacagaaaat ttatattgat
     9001 taaatgcaaa atttttgttg gcctaccccg ccctcccgtg cattactgtt gcattgcgcg
     9061 tttcgcccgc actcggggcg ctcttgcgcc atcccaggga aatccagttt ttattttgaa
     9121 gggggaaatg catgaagaag ctcgaagcaa tcattcagcc ctcacggttt gaatccgtga
     9181 aagatgcgct gcttgagatg ggcgtgggag gcttgacagt gacggaagtg cgcgggcacg
     9241 ggcggcagaa ggg
//
LOCUS       DASYVO010000526         2932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_237322, whole genome shotgun sequence.
ACCESSION   DASYVO010000526 DASYVO010000000
VERSION     DASYVO010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237322"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..>2932)
                     /locus_tag="VGR03_18195"
     CDS             complement(116..>2932)
                     /locus_tag="VGR03_18195"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1996271.1"
                     /translation="PGAVAEFRKAVSLDPKYARAYTNLGSALAKNGELSEAVGAFQKA
                     LALEPDNLVAHLNLGVAFREKGDAKGALVHVRLVAEREPNSADVQYVFGQTLQQSADL
                     QGAIEAFESSVRINPEFHEGYYALGLALKKQAASLRKPRQPLASPADDLYKRGEQAAA
                     RGDLKEAERLLIEAANKDDSHAGAQNLLGFILGQEGDLPSALVHLERAVALRPDLSDS
                     HYNFGVALWYSGSKTKGISELQEAVRLDPAAGASYAFLGTALRETGQLENARRNLQRA
                     IALLPFFPATFIDLGTVYLRAADLDKALAQFEAGLSISASSGPTPDWDTAIADIRGAL
                     AAKPDRAEAHNMLGLLLGRKGADSKEVLAEFREAVRLRPDFADAHNNIGLVLAQGNED
                     KAAVAEFREALRIRHDYADAHANLGTVLISTDSREAVGELQQAVALAPGLLKAQFNLA
                     VAYGTDPSYGPAKEIDQLRKVIATEPTFARARLALGKALLSDGKVEESIGELREAVRL
                     EPQNTKAHYQLGLALARAGQKDQATTELQKSRELASSDDRRQNADLDIAEGRVALDKG
                     ELAQAAAKFQHALELQPESSDAQHFMGVVLEKEGDAQGASVAYRKAVEFNPGDVLARE
                     KVLALSKDEATVEDSQRIAEFEAYIRAGRFQEVEPLLAAYVKEHPKTSWGWYALGYSL
                     FAQRKIGESIQALTKSLQLDIQNAEAHKILGRDLMTIGRFDAAQTEFEQGSRYNPQSA
                     EMHFNLGKLFSVQDSWSAARKEFEAALRIDPSYVESLDGLGFAQEALGDDAGAVASYE
                     KAIAINEARKGQFVSAHVNLSGYYNRKGDSEKALAYARAAWELDPKSDGAWFQQAKAT
                     EAQGRFIDAVDALNRAISLNPRSSSYYYVLAQIYRQLGKVEDSRKALDSFMRLKKETS
                     DLEEMRRNLANGSANTPQPRSQRD"
BASE COUNT          566 a          905 c          817 g          644 t
ORIGIN      
        1 aaacacgctc cccctccgct tgtcgacccc tcaaaacgcg cactgtgccg agcatgtcaa
       61 cagagccaat gcatctgtaa gaaatttgcg gcgtgattgc gcgctgcggg gtcactcagt
      121 cgcgttggct cctcggctga ggcgtgttgg cggagccatt ggcgagattt cgccgcattt
      181 cttccaggtc gcttgtctcc ttcttcagtc gcatgaacga atccagggcc ttgcggctgt
      241 cttccacctt cccaagctgc cggtagattt gggccagaac gtaataatac gaagacgatc
      301 gaggatttaa ggaaatggct cggttcagag catcgaccgc atcaataaac cgcccctggg
      361 cctccgtcgc ttttgcctgc tggaaccagg cgccatcaga tttaggatcg agctcccacg
      421 ctgcgcgggc gtacgcgaga gccttttcgg aatcaccctt ccgattgtaa tacccgctga
      481 ggttcacatg cgccgacacg aactgcccct tcctggcctc gttgatcgca atggctttct
      541 cataactcgc gacagctccg gcgtcatcgc ccagagcctc ctgagcaaaa cccaaaccat
      601 ccaaactttc cacgtacgaa gggtcgatac ggagtgccgc ttcaaattcc tttcgcgcag
      661 cgctccagct atcctggacg gagaatagtt tgcccaagtt gaaatgcatc tcagcggatt
      721 gcgggttgta ccggcttccc tgctcgaact ccgtctgggc ggcgtcgaac cttccaatgg
      781 tcatcagatc gcggccgaga attttgtgcg cctcagcatt ttggatgtcg agttggagcg
      841 atttcgtgag cgcctgaatg gattcgccga tctttcgctg ggcgaacagg ctgtagccca
      901 aggcatacca gccccaagag gttttcgggt gctctttgac ataagcagcc aggagcggct
      961 caacctcctg gaatcgcccc gctcggatgt aggcttcgaa ttcggcgatg cgttgggagt
     1021 cttccacagt agcttcgtcc ttcgaaagcg ccaggacttt ttctctggcg aggacgtcgc
     1081 caggattgaa ctccactgcc tttcgatacg ccacggaagc accctgagca tcgccttctt
     1141 tttcaagaac cactcccata aaatgctggg cgtctgaaga ttcgggctgg agctcaagcg
     1201 cgtgctgaaa tttggcggcg gcttgggcca actcgccctt atcgagcgcc acacggcctt
     1261 cggcaatgtc caaatcagcg ttctgtctcc ggtcgtcgga agaagccaat tcgcggctct
     1321 tctgcaactc agtcgtggcc tgatcctttt gtcctgcccg cgccagagcc agcccaagct
     1381 gatagtgagc cttggtattc tgtggctcaa gccgcaccgc ttcccgaagt tccccgatgg
     1441 attcctccac tttcccgtcg ctgagaagcg ccttacccaa agcgagtcgc gcacgtgcaa
     1501 aggttggttc ggtcgcgatc accttgcgca actgatcgat ttctttcgcc ggtccatagc
     1561 tcgggtctgt gccgtaggcc accgctagat tgaactgcgc tttcaacagt cccggggcga
     1621 gcgctaccgc ctgctggagt tcccctacgg cctctcgact gtcagtggaa atgaggactg
     1681 tgccgagatt cgcgtgcgcg tccgcataat cgtgtcggat gcgcagagcc tcacgaaatt
     1741 cggcgacagc cgctttatcc tcgttgccct gcgctagcac cagccctatg ttgttgtgcg
     1801 catccgcgaa atccgggcgc aggcgcacgg cctcgcggaa ctccgccaga acctccttgc
     1861 tgtctgcacc cttgcgccca agcaggagtc cgagcatgtt gtgcgcttcg gcgcggtccg
     1921 gcttcgcagc caacgctccg cgaatatccg caatcgccgt atcccaatcg ggggtaggac
     1981 cggatgaagc ggagatactg agcccggcct cgaactgcgc tagcgcttta tcgagatcgg
     2041 cggcgcgaag gtaaacagtt ccgagatcga tgaatgtggc ggggaagaac ggcaggagcg
     2101 cgattgcgcg ttgcaggttc cgtcgtgcgt tttctaattg acctgtttct cgcagggcgg
     2161 ttccgagaaa ggcatagcta gcgcctgcgg ccggatcaag tcgaacagcc tcttgcagct
     2221 cggatatgcc tttggtcttc gaaccgctgt accacagcgc aacgccaaaa ttgtagtgtg
     2281 agtcagaaag atccggccgg agggccacgg cgcgctccag gtggaccagt gcagaaggca
     2341 aatctccctc ctggcccaat ataaaaccga gcagattctg cgcgccggcg tgactatcat
     2401 ctttatttgc ggcttcgatg agtagtcgtt cggcctcttt caggtcgccg cgcgcggcag
     2461 cctgctctcc acgcttgtag aggtcgtccg ccggactcgc cagaggctgg cgcggcttac
     2521 gcagcgacgc ggcctgcttc ttcaaggcca agccgagcgc ataataacct tcgtggaact
     2581 ccggattgat gcgcaccgag ctctcaaacg cctcgattgc gccctgaaga tctgcgctct
     2641 gttgtagagt ctggccgaac acgtactgaa catcggcgct gttcggttcg cgctcggcga
     2701 caagccgtac atgcacaaga gcgcccttgg catcaccctt ttcccgaaaa gcgacgccca
     2761 gattcaggtg cgccactaga ttgtcaggct caagcgcgag cgctttttga aaagccccaa
     2821 ccgcctcgga caattcgcca ttcttggcta acgccgatcc caggttcgtg tacgcccgcg
     2881 cgtatttcgg gtcaagcgag acggccttcc tgaattcagc gacggcgccg gg
//
LOCUS       DASYVO010000527         1642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_153793, whole genome shotgun sequence.
ACCESSION   DASYVO010000527 DASYVO010000000
VERSION     DASYVO010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153793"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(556..1572)
                     /locus_tag="VGR03_18200"
     CDS             complement(556..1572)
                     /locus_tag="VGR03_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996272.1"
                     /translation="MKPNTILRGVVAGALLAAFLLPAARPARAGRLGMDIIGLFPKEV
                     GEFAYVDLKKARSIKWYAQLKEQLLPQRFHQFEQFLASAGMDPNSQVDELAWGLVAES
                     AGPDGETSGVPTGETTVGVALGSFNPNSAEAFFKAQKLPTFQARGFTLYAFGSGSGPN
                     DLFFFFIDSNTAAFGHRRLLEKLIEVRYGTEEGLLRNDKLFPLINEANGSSVVWAVLN
                     PSYARLAMHQLAPEVEQFPEAAKLVQNLKSLIINLDAGSGVDGKFQAIAASTEDANTL
                     AQLLTAGFLYRRYQAQQSNAELGQLLDLARITPSGDRVVLRMSLSDDQMTSLIRQNVF
                     TLRM"
BASE COUNT          316 a          466 c          538 g          322 t
ORIGIN      
        1 gattatacgc cttggaattc cacttggatg cagcggaatt ggcgcaagag gtgccggacc
       61 tggcgaccgc tcttggattg ggtttccgtc gccttcagcg taggaatgct tcgtcggaga
      121 acatagggcg cgcgtagagg caatgttcgc aaacagagaa cagtcgtgtt gcccggtgga
      181 atcaggtcgg gtaaagtggc ctcggaggtg gagatggaag gatcggtcgt ttgtcgccag
      241 tgcgataaaa cggaacacga atgcgcgtgc gagaagtatt gctcgatctg caaggggcag
      301 ctcgaaatcc gcttgtgcgc cgacgggctg tactactgcc ccgcttgccg ggaggcatgc
      361 gacgtatcga tcgcggacgt ccgcgggcaa taagttccac ggtttggacg caggtctcga
      421 cgatagtgcc cgtctgatcg cgggcgcggc ctggctgaag cgacagtgga cagcatgcgg
      481 atgcagggtc ggcgaataca atcccccaaa agctggctgc gcttgtccgc tcctctgaat
      541 tcgcttgcgc cggagttaca ttcgcaacgt gaacacgttt tggcggatga gcgaggtcat
      601 ctggtcatcg ctcagagaca tgcgcaacac gacgcgatcg cccgagggag tgatgcgcgc
      661 caggtccagg agttgcccga gctcggcatt gctctgctgc gcctggtagc gcctgtaaag
      721 gaagcccgca gtgaggagtt gtgccagcgt gttggcgtcc tcggtggacg ccgcaatggc
      781 ctggaatttg ccgtccacgc cgcttccggc atcgagattg atgatcagac tcttcaggtt
      841 ctggacgagc ttcgcggctt cggggaattg ctcgacttcc ggcgcaagct ggtgcatcgc
      901 caggcgcgcg taagagggat tcaggaccgc ccagaccacg ctcgaaccgt tcgcttcgtt
      961 gatcagcggg aataacttgt cgttgcgcag gagcccctct tcagtgccgt agcggacttc
     1021 gatgagcttc tcgagcagtc tgcggtgtcc gaacgccgct gtattcgaat cgatgaaaaa
     1081 gaaaaagaga tcgttcggcc cggagccgct gccgaatgcg tagagcgtaa acccccgcgc
     1141 ttggaaggtg ggcagctttt gcgccttgaa gaaggcttcc gcggaattgg gattaaagct
     1201 gccgagcgcg acgccgacgg tggtttcgcc ggtcggcacg ccggacgttt caccgtccgg
     1261 cccggcggac tcggcgacga ggccccaggc cagctcatcc acctgcgagt tcggatccat
     1321 cccggcggag gcaaggaact gctcgaactg gtggaagcgc tgcggaagca gctgctcctt
     1381 cagttgtgca taccacttga tcgagcgcgc ctttttcagg tccacgtagg cgaattcgcc
     1441 gacttccttc gggaacagcc cgatgatgtc catgcctagg cgtcccgcgc gggcgggccg
     1501 cgccgccggc aggaggaacg ccgcgagaag ggcgccggcc accacgccgc gtaagatcgt
     1561 attcggtttc atgagaggct ccttttggga ccctggtaaa actgtatcac gctcccaagg
     1621 cgcgaggcca atctccaatg cg
//
LOCUS       DASYVO010000528        10465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_193176, whole genome shotgun sequence.
ACCESSION   DASYVO010000528 DASYVO010000000
VERSION     DASYVO010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10465
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193176"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VGR03_18205"
     CDS             complement(<1..665)
                     /locus_tag="VGR03_18205"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996273.1"
                     /translation="MTHSSKRLLKFMLSGLAQRHALFFSLLLCFLTSLPLLAEHTRRW
                     RQSTYEEFLKGTAHGVAVRSDGRLELAPKVTLLADADASYLWSVRLDPKGTLYAAGGS
                     PAKVFRFDSNGKPAVVFDSTDLSAQAIAFDSKGTLYVGTSPDGKVYRVSGSGEKSVFF
                     DPKTKYIWDLAFSPDGTLYVATGDKGQVFAVAPDGKGELFYSSDEAHIRVLAFDSKGN
                     LIAG"
     gene            complement(674..2485)
                     /locus_tag="VGR03_18210"
     CDS             complement(674..2485)
                     /locus_tag="VGR03_18210"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HEV1996274.1"
                     /translation="MLTVCFRLPKSRCLLFLALLAFPAALCLGSGVAIAQESNEVLPL
                     SQVRAGMQGYAYTIFAGDQIEKFNLEVIGVLDNFLGPKQSIILVQLKGPKVEHTGVVA
                     GMSGSPVYLDGKLAGALSLKLGIFTKEPIAGVTPIQDVLNPPSQPATSQAAVQQLGLP
                     SEASTRTGLPSGSALEPIETPLVFSGFQPAALQQFANQLQGYGFVAAQGGTASPRPDD
                     GRLVAGDMAGMVLVQGDASINSACTVTAVQADRVYLCGHPFLSLGDIQIPMARSRVVT
                     TLSSDLASTKIVNVGGSIGTITGDHLTAVTGKLGAPPAMIPLDLTLLVAGAETAKQKT
                     LHFELVNHPKLTPLLVALTTFNGLTQNSLYGEGTTLHLSGEIHLQGHAPVQIENTFAP
                     GDSFSPDGLPIALAMQSVFTRLFVNTFEPAKVERITLRVESVPGRKSFTIESAWLEKG
                     EAAPGETLRVRVLLRPYRGAARVEETTVRVPEQVVRGTTLRILVSDADLLNRASRGFA
                     FAGAGSGPNGLDQLIALLNRERRNDRLYVGLFAPSPTLLWDDKELPNIPLSQINVVDG
                     RPTPGSVQVLRESLASESSIPLGGPVSGVISLNLQIR"
     gene            2963..4240
                     /locus_tag="VGR03_18215"
     CDS             2963..4240
                     /locus_tag="VGR03_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996275.1"
                     /translation="MRRRLAVLVMMLFAASAAWAQSPPPLDQAEQVRMLIDQVRKLEK
                     RVAELEARPSAAAAPSESAANVPAAPKPELAEAAPSPGPGQGHQHEGRDEQAAVRQME
                     THYPSLQIRGFGDVDFSATDQKGTTSGFNLGQLDLHLTSPLSRKVSYFGEMTFNAQPT
                     GYTVEVERSIIRYDYNDFFKISFGRYHTPIGYWNTAFHHGAWLQTSISRPDMIRIGGT
                     FIPVHFVGFLAEGNIPSGGAGLSYSAGVGNGRGAIISRPGDAGDNNNNRAWLANLYSR
                     PVKLYGLQMGVSVYRDKITLPTGNNFREWISAAHVAWTKETPEFLAEFANVNHRNILT
                     NFVTNSQAFYAQVGYRLPWLERSLKPYYRFEHTHTPLSEQVFTNQDLVGSILGLRYDI
                     TNYAAFKAEYRNSKRLPTEPRVNGAFFQTDFTF"
     gene            4253..4750
                     /locus_tag="VGR03_18220"
     CDS             4253..4750
                     /locus_tag="VGR03_18220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996276.1"
                     /translation="MNATIKRVVTILWTTISICTVLPHPSAAQTTRGTDIAVVVHPDT
                     PVSDLSLSEVRKVLLGERQYWNSKLPVVLLIRAPVARERDVVLRVIYQMSEAQFKQYW
                     VAKIFRAEVASPPKIVYSNDLQHDLITAMPGAIAFVDAKNVRPGLKVLRVDGHLPGDK
                     DYPLR"
     gene            complement(4810..7230)
                     /locus_tag="VGR03_18225"
     CDS             complement(4810..7230)
                     /locus_tag="VGR03_18225"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV1996277.1"
                     /translation="MIGLTVSHYRILEKLGGGGMGVVYKAEDLRLGRHVSLKFLPEQL
                     SRDPLAIERFQREARAASSLNHPHICTIYDVDQYEGQNFIVMEFLEGQTLKHRIAEKP
                     IDLERVPEYGYQMADALEAAHAKAIIHRDIKPANIFLTDRGQVKLLDFGLAKLLPERK
                     GANRVTQGSRAFGLTTQDAHLTSDGVALGTVAYMSPEQVRGQELDERTDLFSLGLVLY
                     EMSTGQRAFTGNTSGVIFDGILNRAPTPPARLNPVIPVQLEQIIGKALEKDRELRYRT
                     ASDMRADLQRLKRDTDSARAMPYSTGQASRQKLRRHWPHLVWGGILALLLLLFGLNVG
                     NLRDRVFDGTSQARIDSIAVLPFANLSNDPKTEYLSDGITESLINSLSQLPNLAVMSR
                     NTVFRYKGQPADPQKVGRDLHVRAILTGRLIQTGDELLISVNLEDVQNSRQLWGEQYN
                     RKLSNLVSVQQEIAGDIYERLRPRLAGEEKKLLAKRPTENVEAYQLYLQGLFYWNKWT
                     QADFKKAADYFTLAVQKDPRYALSYAGLADTYSLLGDAGYLPPSEAWPKAKAAAMQAL
                     EIDDTLAEAHTSLGLVKEHFEWDWTGAEREFKRAIELNPNSPTAHHWYGDYLANMGRS
                     EEGLRETKKAQELDPLSLIINTSLGWQLYVARHNDQAAEQLRKVLDIDAKFAPARRML
                     EEVYAEMGKQKEAVAEREKILSLSGSPELAASIEEDFSKSGYKGVLQSWLDGLTEISK
                     HGYVSYYSIAQAYMRMGEKEKTFGWLEKAYEEHDSGLVSLAVEPMFDPVRSDPRFRDI
                     LRRIKLAN"
     gene            complement(7349..8185)
                     /locus_tag="VGR03_18230"
     CDS             complement(7349..8185)
                     /locus_tag="VGR03_18230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996278.1"
                     /translation="MSARSTFIHLAAKAGILFGALFLVSVLLAAIPSHRAPAAPVQDA
                     QQSSGSQPQESQAMDHSKMSGMEMDDAKANEAHAVHDMTPGHMDAHNLHMHMTAMRPQ
                     TPEDTARANEIVTQLRSGIEKYKDYHAAMNDGYKIFLPNLPQPEYHFTKYVNGFLEAF
                     SFDPARPTSLLYRKTSDGYELVGAMYTMPKRATEDQLNARVPLSVAMWHLHTNLCMPQ
                     KGQFRNADLSKFGLKGSIATQEACDAAHGRFQPVIFGWMVHVYPYEDSADKVFAMHHP
                     ME"
     gene            8375..9685
                     /gene="aroA"
                     /locus_tag="VGR03_18235"
     CDS             8375..9685
                     /gene="aroA"
                     /locus_tag="VGR03_18235"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV1996279.1"
                     /translation="MKKETIQPAKHLTGGLDLPGDKSVSHRYAMLAALAEGTSELRHF
                     STAADCHSTLACMSALGAEVKTDKDTVRVIGRGPRGLKSSWRALDAGNSGTTMRLLVG
                     ILAGQQFSSKLTGDDSLQKRPMKRVIGPLRDMGADIRSRDDNFAPLEIRAARLKAIDY
                     KMPMASAQVKSAVLLAGLFADGVTSVTEPARTRDHTELALEEFGAAIEKDGRTIRIHG
                     LGGANGSGKLIAKSLDIPGDLSSAVFFIAAASLFPDSNVLIHNVGLNPTRTAILDLFA
                     SMGAAIQILGLKSAHGEIVGDLSVKGVSLKGGVIAGEQIPLMIDELPMLAALGPYTEE
                     GIEIRDAAELRVKESDRIAALVENLRRMGASVEERPDGLKVEGRRAGKLRGAEIEPRG
                     DHRIAMAFAVAGLAAEGNTVIRDADCAGVSFPTFFLELNRLAER"
     gene            complement(9753..>10465)
                     /locus_tag="VGR03_18240"
     CDS             complement(9753..>10465)
                     /locus_tag="VGR03_18240"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEV1996280.1"
                     /translation="GTGGDVNIETKKSDIKVSDTNGNIKISGHGGEINVSGATGGLTI
                     DGEFYGAIRADKVAKGVRFISQRTDLTLSQLAGHMEAGPGNLEIFDAPGNISLRSQDD
                     VTLENAGGKLKVDNRRGNVEVRFSAPPKDDIDITNSSAGITLSLPGSSSFEIVADCHS
                     GDIDSEFQADSLKLTSGTGSGDSHLEGKYGSGRGAKIILKTSYGSISLRRTSSDIPAP
                     PKPPRVKTPPPPEKSEEN"
BASE COUNT         2192 a         2908 c         3177 g         2188 t
ORIGIN      
        1 ccggcgatga gatttccttt tgaatcgaaa gccagaacgc gaatgtgagc ttcgtcgctg
       61 gagtagaaga gttcaccttt gccgtcgggc gcgacggcga agacctgtcc tttgtcaccg
      121 gtcgcgacgt aaagagttcc gtcaggcgaa aaagcgaggt cccagatata tttcgttttg
      181 ggatcgaaaa aaacggactt ctcgcccgaa cccgacactc ggtaaacctt gccatcggga
      241 gaagtgccga cgtagagagt gcctttggag tcaaaggcga tcgcttgtgc agagaggtcc
      301 gtggaatcga ataccacggc gggtttgccg ttcgaatcga aacgaaatac ttttgcaggc
      361 gagccgcctg cggcgtagag cgtgcccttg ggatcgaggc ggacggacca aagataggaa
      421 gcatctgcgt cggcgagcaa agtgaccttg ggcgcgagtt cgaggcggcc gtcgctgcgc
      481 accgccactc catgcgcggt gcctttcagg aattcttcgt aagtggactg gcgccagcgg
      541 cgggtgtgct cggcaagaag aggcaacgaa gtaagaaagc aaagaagcaa ggagaaaaaa
      601 agagcgtggc gctgcgccag gccggacaac ataaatttga gcaaacgctt ggacgaatga
      661 gtcatcgaaa tgttcaccgg atttggagat tcagagagat aacgccggag acagggcctc
      721 cgagcgggat ggacgattca ctggccaacg attcgcgcag cacctggacg ctgccgggag
      781 tggggcggcc gtcgacgacg ttgatttgcg agagcggaat gttcggtagt tccttgtcgt
      841 cccaaagaag cgtgggggaa ggagcgaaca gaccaacgta aaggcggtcg ttccggcgct
      901 cgcgattcaa gagcgcgatg agttgatcca ggccgttggg gccgctgccg gcgccggcaa
      961 acgcgaagcc acgcgaggcg cgattcaaca aatcggcgtc gctgaccagg atgcggagcg
     1021 tggtaccgcg cacaacctgt tccgggacgc gaacggtggt ctcttccacg cgggccgccc
     1081 cgcgataagg acgaagcagc acgcggacgc gaagagtctc gccgggagcg gcttcgcctt
     1141 tttcgagcca tgcgctttca atggtaaatg attttctgcc cggaacgctt tcgactcgca
     1201 gggtgatgcg ctccaccttg gcgggttcga acgtattgac gaacaggcgt gtaaaaacac
     1261 tttgcatcgc caaggcgatg gggaggccat cgggagagaa agaatcgccc ggagcgaaag
     1321 tgttttcgat ttgaaccggc gcatgacctt gcaggtgaat ctcgccggaa agatggagcg
     1381 tggtgccctc accgtacaaa gaattctgcg tgagaccgtt gaaggtggtt agggccacga
     1441 ggagcggcgt gagcttgggg tgattgacga gttcgaagtg gagtgttttt tgcttcgcag
     1501 tttcggcgcc agcgaccagc agggtcagat ccaggggaat catcgcggga ggagcaccga
     1561 gtttgccggt gaccgcggtg agatgatccc cggtaatggt gccgatggag ccgccgacgt
     1621 tgacgatttt ggtggaagcc agatcggaag aaagcgtggt gacgacccgg ctgcgcgcca
     1681 tgggaatctg aatgtcaccg agactcagaa aaggatggcc gcagagatac acgcggtcgg
     1741 cctgcaccgc cgtgaccgtg caggcggagt tgatggaggc atcgccctga acaagaacca
     1801 tgccggccat gtccccggca acgagacgtc catcatcagg cctcggcgac gctgtgccgc
     1861 cttgcgccgc gacgaagccg tagccttgca gctgatttgc gaattgctgg agcgcggccg
     1921 gctggaagcc ggagaaaacg agcggcgttt caatgggctc gagagcggag ccagaaggca
     1981 gcccggtccg cgtggaggct tcactgggaa gcccgagttg ctgtactgcg gcttgcgacg
     2041 tggcaggctg ggagggcggg ttcagcacgt cctggatggg cgttacgcct gctatgggtt
     2101 ccttggtgaa gatgcccagt ttcagcgaca gcgcgcccgc tagtttgccg tccagataga
     2161 ccgggctgcc gctcattccc gcgaccacac cagtgtgttc gactttcggc cccttcaact
     2221 gtacgaggat gatggactgc ttgggaccca gaaaattgtc cagcacgccg atgacttcga
     2281 ggttgaactt ctcgatctga tcgccggcaa agatcgtata ggcgtacccc tgcatgcccg
     2341 cgcgaacctg gctgaggggg aggacttcgt tcgattcttg agcgatggcg acgccagatc
     2401 ccaggcacag agcggccggg aacgctaaga gtgcgaggaa cagaaggcag cgagacttgg
     2461 gcagacgaaa gcagaccgta agcatgaatt ggtaatgata cgcggcgggg taactctagg
     2521 caatcaaacc taagatgcgc tgttgatagg agaagttagc taggagtcct agaacttgct
     2581 ctgttggcta aaatgcctcg cgaatgcgcg atcgggattc gcaagtaaga ctgagttgtc
     2641 gagcccgcgg gttgaacgaa tcgcggtggt gcgcggataa tactctgtca gctgaagcgt
     2701 attccgccac gccgcgtaaa ggatgtgcaa ggcactaccg gcggtagaca gatcgggcag
     2761 acgcaagaaa gtaagttttg acaatcgagt cgtgaagcat acaatacgct ccactcttcg
     2821 gcacattcca gggtgacgaa gggctcccga catttaagcc gtgatttgct gaagcaggcc
     2881 gggggagtct gattccagat ttcactatgg atgacttctt cccctcgggt aaaaagccga
     2941 gaaattggta gaggagggta tcgtgcgccg acgactggcg gtcctcgtga tgatgctatt
     3001 tgcagcaagc gctgcctggg cacaaagccc gccaccgctc gaccaggcag agcaggtgag
     3061 gatgctgatc gatcaggtcc ggaaactcga aaaacgcgtt gcggagttgg aggccaggcc
     3121 ttccgcggct gccgctcctt cggaatcggc cgcgaatgtt cccgccgcgc caaagccgga
     3181 attggccgaa gcggctccat cccccgggcc ggggcaaggc catcaacacg aaggccgcga
     3241 cgaacaagct gcggtccgcc aaatggagac tcactatccg tctctccaga ttcgcggttt
     3301 tggagacgtg gatttttctg cgacggacca gaagggaacc acgagcggct tcaatctcgg
     3361 ccagctcgat cttcacctca cctcgccact ttctcgaaag gtcagctatt tcggtgaaat
     3421 gactttcaat gcccagccga ctggctacac cgtggaagtg gaacgctcca tcattcggta
     3481 cgactacaac gattttttca agatctcctt cgggcgttat cacacaccca tcggctactg
     3541 gaataccgcc ttccatcacg gcgcatggct ccaaacgagc atcagccgtc ccgacatgat
     3601 caggatcggt ggaaccttca tccctgtcca tttcgtggga tttctcgccg aaggcaatat
     3661 cccctccgga ggggcggggc tgagctacag cgccggtgta gggaatggcc ggggggcgat
     3721 catcagtcgt cccggcgacg caggcgacaa caacaataat cgcgcgtggc ttgccaacct
     3781 ctattcccgg cctgtcaaac tctacggatt gcaaatgggc gtttccgtgt accgagacaa
     3841 aatcacgcta cccacgggaa ataatttccg cgagtggatc tcggccgctc atgtagcctg
     3901 gaccaaggaa accccggaat tcctggcgga atttgccaac gtcaaccacc gcaacattct
     3961 taccaacttc gttaccaaca gccaggcgtt ctacgcccag gttggctacc ggctcccatg
     4021 gctggaaagg tcgttgaagc cctattatcg cttcgagcat acacacacgc cgctgagcga
     4081 acaggtcttc acgaatcagg acttggtggg ctcgattctt ggtctgcggt acgacatcac
     4141 aaactatgcg gctttcaaag ctgagtaccg caatagcaag cgcctaccca cggaaccgcg
     4201 tgtgaatggg gcttttttcc agacagactt tactttctga gcgagctacc caatgaatgc
     4261 cactatcaaa cgcgtcgtca caattctctg gacaacgatc tcgatttgta cggtccttcc
     4321 gcatccttcg gcagcccaga caacgcgcgg gacggatatc gccgtcgtcg ttcatccaga
     4381 cactcccgtc agtgatctca gccttagtga agtgcgcaag gtccttctgg gcgagcggca
     4441 atactggaat tccaagctcc ccgtggtatt gctcattcgt gctcccgtcg cccgggaacg
     4501 cgatgtagtc ttgcgcgtga tttatcagat gtcggaagcc cagttcaaac agtattgggt
     4561 ggccaagatc ttccgcgcgg aggtcgcttc accaccgaaa attgtctact ccaacgatct
     4621 gcagcacgac ctcatcactg ccatgcctgg cgccatcgca tttgtggatg cgaaaaatgt
     4681 gcgccctgga ttgaaagtgt tgcgcgtgga tggccatctg ccgggggaca aggactatcc
     4741 tttgcgctag tcgagtgcac tccctgcacc ttggggctac ggacaaaagg gccgctgtgg
     4801 ggccaccctc taatttgcca gcttaatccg cctgaggatg tcccggaatc gggggtccga
     4861 tcggaccggg tcgaacatcg gctcgacggc gagcgaaaca agcccgctgt cgtgttcctc
     4921 ataggccttt tccagccagc cgaatgtttt ttctttctcg cccatgcgca tgtaggcctg
     4981 agcgatgctg tagtacgaga cgtagccatg cttggagatt tccgtcaggc catccagcca
     5041 gctttgcaag acacccttgt acccggattt ggagaaatct tcttcgatcg aggcggcgag
     5101 ttcagggctt ccggagagtg agaggatttt ttctcgctcc gccacggcct ctttttgttt
     5161 tcccatttcc gcgtagactt cctcgagcat gcgcctcgcc ggtgcaaact tcgcatcaat
     5221 gtcgaggacc tttcgcagtt gttcggcggc ttgatcgttg tggcgcgcga cgtacaactg
     5281 ccagcccaaa ctggtattga tgatgagcga gaggggatcc aattcctggg ccttttttgt
     5341 ttcgcgcagt ccttcctcgg agcgccccat gttcgccaag tagtccccgt accagtgatg
     5401 ggcggtcggg gagtttggat ttagctcgat ggcccgcttg aactcccgct ccgcaccggt
     5461 ccaatcccac tcgaagtgct ctttcacgag acccagggac gtgtgcgctt ccgcgagcgt
     5521 gtcgtcaatc tccagcgcct gcatcgcggc ggcttttgct ttcggccagg cctccgaagg
     5581 cggaaggtaa ccggcgtctc ccagcagact gtatgtgtct gccagccccg cgtaggacaa
     5641 tgcgtaccgg ggatcctttt gcacggccag cgtgaagtag tctgcggctt tcttgaaatc
     5701 cgcctgcgtc cacttgttcc agtaaaacag gccctgcagg taaagttgat acgcctccac
     5761 attttcggta ggccgcttcg ccagcaactt tttttcttcc ccggcaagac gcggacgtaa
     5821 gcgttcgtaa atgtcccctg caatctcttg ctgcacggac acgagattgg aaagcttgcg
     5881 gttgtactgt tcaccccaga gctgccggct gttttgcacg tcttccagat tcacgctgat
     5941 caacaattcg tcacccgtct ggatgagtct ccccgttaga atggctcgga catgcaggtc
     6001 gcgccccacc ttttgcgggt ctgccggctg gcctttgtag cggaaaaccg tgttgcgcga
     6061 catgaccgcc aagttgggca gttgggagag gctgttgatc agactctcgg tgattccgtc
     6121 gctcagatat tctgttttcg gatcgttgct caaattggcg aagggcaaga cggcgatgga
     6181 gtcaatgcgg gcctggcttg tcccgtcaaa cacgcggtcc cttaaattgc ccacattcaa
     6241 gccgaatagc agcaatagga gagcgagaat tcccccccac acaagatgtg gccaatgccg
     6301 ccgcagcttt tggcgcgagg cttggccggt tgaatacggc atggccctgg cggaatccgt
     6361 gtcgcgcttc aaccgttgta gatccgcccg catgtccgaa gcggtgcggt accgcagctc
     6421 ccggtctttc tccagcgcct tgccgataat ctgttccagt tggacaggga ttacggggtt
     6481 aaggcgggct gggggagtcg gcgcgcggtt caagatcccg tcaaagatga cgccagatgt
     6541 atttcccgta aacgccctct ggcccgtgga catttcataa aggaccagcc ccagggagaa
     6601 cagatccgtg cgttcatcga gttcctgacc tcgaacctgc tcgggggaca tgtaggccac
     6661 ggtacccagg gccactccgt cgcttgttag atgggcgtct tgagtcgtga ggccgaaagc
     6721 tctcgaaccc tgcgtgaccc gattcgcgcc ctttcgctcg ggcagaagct ttgcgaggcc
     6781 aaaatcgagt agcttcacct ggccgcgatc ggtaagaaaa atattggccg gcttgatgtc
     6841 gcggtgaatg atggctttgg cgtgggccgc ttccagggca tctgccatct ggtagccgta
     6901 ttcgggcacg cgctcgagat caatgggttt ctctgcaatc cggtgcttga gggtctgccc
     6961 ttcgagaaat tccatgacga tgaagttctg accttcatac tggtccacgt cataaatcgt
     7021 gcagatatgg ggatgattta gcgacgaggc ggcccgtgcc tcccgctgaa atcgttcgat
     7081 cgccagggga tccctggaaa gctgttcggg gagaaacttc aatgaaacat ggcggccgag
     7141 tctaagatcc tcggctttat acaccacgcc catcccgccg ccgcccagct tctccaggat
     7201 gcggtaatgg gagacggtta gcccgatcat agagaggaaa ccgccattgg cacgtcatat
     7261 tagggtgtgc ctggagacaa gtcaacgcaa agggcgccag agcgttcaaa tggtgaagcg
     7321 gtagatcggc agccgagccg acgcctggtt actccatggg gtggtgcatg gcgaagactt
     7381 tgtcggcgga atcttcgtag gggtacacgt gcaccatcca gccgaagatg acgggctgga
     7441 agcggccatg cgcagcgtcg caggcttctt gcgtggcgat cgatcccttc aatccaaatt
     7501 tggacaaatc ggcgttgcgg aactgaccct tttgcggcat gcagagattg gtgtgcaaat
     7561 gccacatggc cacactgaga gggacgcgcg cgttgagctg atcttccgtc gcgcgcttgg
     7621 gcatggtgta catggcgccg accagctcgt acccgtctga agtttttctg tagagcaggg
     7681 aagttgggcg cgctggatcg aaagaaaaag cttcgaggaa gccgttaacg tatttcgtga
     7741 agtgatattc gggctgcggc aggttgggca gaaaaatttt gtaaccgtcg ttcattgcgg
     7801 cgtgatagtc cttgtatttc tcgatgccgg agcgaagctg cgtgacgatt tcgttggccc
     7861 gcgcggtgtc ctctggtgtt tgcggacgca tcgccgtcat gtgcatgtgg agattgtgcg
     7921 cgtccatgtg cccgggcgtc atatcgtgaa cggcgtgagc ttcgttggcc ttggcatcgt
     7981 ccatttccat gccggacatt ttgctgtgat ccatcgcctg cgattcctgc ggctgcgagc
     8041 cggatgattg ctgggcatcc tgcacaggcg ccgcgggcgc gcgatggctg gggatcgcgg
     8101 caagcagaac gctgaccaga aaaagtgcgc cgaataagat gccggctttg gcggcgagat
     8161 gaatgaacgt gctgcgggct gacatgacgt ttctcctcac ctaataatac gcatcgcgaa
     8221 gccacggaat ttcagcgcgt ggtacaggac gtgtccggca aaaaactggc cggaaggaca
     8281 gggccaggtg ctaaaaatag aaatttgaaa atggaaattg ggatgcggat cttgcaattc
     8341 gagccagggc gacgccgcgt gttagaattt cgtcgtgaaa aaagagacca tccaacccgc
     8401 aaaacacctg accggcggac tggatctgcc gggcgataag tccgtttcgc accgatacgc
     8461 catgctcgcg gcactggccg agggcaccag cgaactgcgg catttttcca cggccgcgga
     8521 ttgccacagc acactggcat gcatgagcgc gctgggcgcc gaagtcaaaa ccgacaagga
     8581 tacggttcgc gtgatcggac gcgggccgcg cggcttgaag agcagttggc gcgcgctcga
     8641 tgccgggaat tccggaacga ccatgcggct gctggtggga attctggccg ggcagcaatt
     8701 ctcctcgaag ctgacaggag acgactcgct gcaaaaacgg cccatgaagc gcgtcatcgg
     8761 gccgctgcgg gatatgggcg cggacattcg ctcgcgcgac gataattttg ctccgctcga
     8821 aattcgcgca gccaggctca aggccatcga ttacaaaatg cccatggcca gcgcgcaggt
     8881 aaaatccgcg gttttgcttg cggggctgtt cgcggatggc gtgaccagcg tgacggagcc
     8941 ggcgcgaact cgggatcaca ccgagctggc cctcgaagaa tttggcgcgg ccatcgagaa
     9001 agacggacgg acgattcgaa ttcacggact cggcggcgca aatggcagcg gcaagctgat
     9061 cgccaaatcg cttgatattc ccggtgattt gtcttcggcc gtttttttta tcgccgcggc
     9121 gtcgctcttc ccggattcga acgtgctgat tcacaatgtc ggattgaatc cgacaaggac
     9181 agccattctg gacttatttg cttcgatggg tgccgcaatt caaatcctcg gactcaagag
     9241 cgcccacggt gaaatcgttg gcgacctctc ggtgaaggga gtatcactga aaggtggcgt
     9301 gatcgcgggt gaacagattc cgctgatgat tgacgagttg ccgatgctcg ccgcgctcgg
     9361 cccgtatacg gaagaaggga ttgagattcg cgacgcggcg gaactgcgag tgaaagagag
     9421 cgaccgcatc gccgcgctcg tggaaaattt gcgtcgaatg ggcgcatccg tggaagagcg
     9481 cccggacggg ttgaaagtgg aaggccgtcg cgcaggaaag ttgcgcggcg cggagatcga
     9541 gccgcgcgga gatcaccgca tcgcgatggc gttcgccgtc gcgggcctgg cggccgaggg
     9601 aaacacggtg attcgcgatg cggattgcgc aggcgtttcg ttccccacgt tttttctgga
     9661 attgaaccgc ttggcggagc gttagtcggc cgagtacatc aggcccgctc ttcccatcga
     9721 actcgagccg ccatagtctc ctgccatcgc gctcagtttt cttcggactt ctccggcgga
     9781 ggaggcgtct tcacccgcgg cggtttcggc ggcgcgggaa tatcggagct cgtcctgcgg
     9841 agcgaaatcg atccataact cgtcttcaaa ataattttcg ccccgcgccc gcttccatac
     9901 tttccttcca ggtgagaatc gcccgatcct gtccccgaag tcagcttcag cgaatccgcc
     9961 tggaactcgc tgtcaatatc tcccgaatgg caatccgcga caatctcaaa gcttgaggac
    10021 cccggcaggc tgagcgtaat ccccgcggaa gaattagtga tgtcgatgtc gtctttcggt
    10081 ggagccgaga aacgaacttc cacgtttcct ctgcgattgt ccaccttcag cttgccgccg
    10141 gcgttctcca gcgtgacgtc gtcctgcgaa cgcaagctga tattcccggg agcgtcaaag
    10201 atttcgaggt tgcccgggcc ggcctccata tggccggcga gttgcgagag ggtcagatcg
    10261 gtgcgctgcg aaatgaagcg cacgcccttc gcaaccttgt cggcgcggat agccccgtaa
    10321 aattcgccgt cgatcgtcag cccgcccgtg gcacccgata cattgatttc cccgccgtga
    10381 ccggaaatct tgatgttgcc attcgtgtcg gaaactttga tgtcgctctt cttggtctcg
    10441 atattgacat cgccccccgt gccgc
//
LOCUS       DASYVO010000529         4033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_237375, whole genome shotgun sequence.
ACCESSION   DASYVO010000529 DASYVO010000000
VERSION     DASYVO010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237375"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1028)
                     /locus_tag="VGR03_18245"
     CDS             complement(45..1028)
                     /locus_tag="VGR03_18245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV1996281.1"
                     /translation="MRYFFLLICASAVFASATFAQIPIPNSAPPPPGQDPNAGKQGSS
                     IKVDVNLVVLHTTVVDDRQRFADGLKSENFRVFEDKVEQKLSVFKREDVPVSMGLVID
                     NSGSMRDKRSRVNEAAITLVQASNPQDEAFVVNFNDDFYLDLDKDFTSSIPELKEALE
                     RIDSRGSTALRDAILGSLDHLKKGSKDKKVLLVVTDGEDNASRNSLEKMIREVQKTDT
                     VIYTIGLLGQENKKEAKRARKVLEQIAAASGGLAFFPENVDDVHSICEQVAHDIRNQY
                     ILAYYPTNSSHDGTFRAVTVAVIPPRGRGKLLARTRNGYYAPAAAATTSGN"
     gene            complement(1054..2112)
                     /locus_tag="VGR03_18250"
     CDS             complement(1054..2112)
                     /locus_tag="VGR03_18250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV1996282.1"
                     /translation="MKRDLPGFRVLAFLFHVLLVVCLFVLHAVLPAAAQSTSDENKPK
                     DEAKRGEVKEVKNDDVRKDTAGTPFKPGGTIHFDVDLALVNVTVTDPYNRLVTGLEPD
                     NFRVFEDNIEQEVVNFSAEDVPISIGVIFDFSGSMSNKVGKAREAAVQFFKTANPQDE
                     FFLVSFNERAELTSSFTNSVEDLQSRLMLTAPKGRTALLDAIYLGLSQMRGAHNAKRA
                     LLILSDGGDNHSRYNESDIKRLVKEADTQLYAIGIFDPLGYRNRSPEELSGPSLLGEV
                     TEMTGGRVFAVEKLDELPDIASKIGMELRNQYVLGYRPSNKAHDARWRKLKIKLRAPK
                     GLPPLSVYSKTGYYAPSH"
     gene            2361..3086
                     /gene="kdsB"
                     /locus_tag="VGR03_18255"
     CDS             2361..3086
                     /gene="kdsB"
                     /locus_tag="VGR03_18255"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEV1996283.1"
                     /translation="MVVVIPARYGSTRLPGKPLVLLAGKPMIQRVYERAKLAKRADRV
                     IVATDDERIVKAVEEFGGEARMTRTEHRTGTERVAEVAAREEGDVFVNVQGDEPLLDP
                     NAIDVAVNALLEEPQASISTVATPIKTPGDIMDPNVVKAVLDFDGNAIYFSRAPIPWV
                     RDTASKIQVRHLKHLGLYVFQREALLEYPTLPQGELERIEQLEQLRWMENGWKIRVAE
                     VEHDAVSVDVPEDVARVEKLLQK"
     gene            complement(3139..>4033)
                     /locus_tag="VGR03_18260"
     CDS             complement(3139..>4033)
                     /locus_tag="VGR03_18260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996284.1"
                     /translation="YQLDTNGDGTNFGRGTANIAGHNFAFYIVDATRLKLLGTDFPAA
                     FSGEAFAQQNISFTAASLNSGFAFLIGGAIQKQSISGPIATAGRFTADGAGNLSEIFL
                     DENNNGALTLLPSQGGTVTGTYAVDANGLGGGTATWTDTKVGTFSFIFYLISPTKAVF
                     QETDSGITSDGTLLAQTAGPVSLATLAGDFAFVWTGVSTDEEDFVGQLKLTSASGNNA
                     SGIMDFNEFGAGKQFFNIQFSGPLTITAPGTGPNTLTLTTTFPSPTTFNFTAYAVDSN
                     TVFLVGVDNNRVLAGSIARQP"
BASE COUNT          862 a         1019 c         1318 g          834 t
ORIGIN      
        1 cgcagcatgc tgcgccccta caaagacaaa caactgaaga atcgtcagtt gccggaggtc
       61 gtggcggcgg cagcgggggc gtagtagccg ttgcgggtgc gggcgaggag cttgccgcgg
      121 ccgcgcgggg gaatcaccgc gacggtgacg gcgcggaaag tgccgtcatg gctggagttg
      181 gtgggatagt aggccaaaat gtactggttg cgaatgtcgt gcgcgacctg ttcgcaaatg
      241 ctgtgcacgt catcgacgtt ttccggaaaa aaggccaggc cgccggaggc cgccgcgatt
      301 tgctcgagca ctttgcgggc gcgcttggct tccttcttgt tttcctgacc gaggaggccg
      361 atggtgtaaa tcaccgtatc ggtcttctgg acttcgcgaa tcattttctc cagagaattg
      421 cggctggcgt tgtcttcgcc gtcggtgacg acgaggagaa ctttcttgtc cttggagcct
      481 tttttaagat gatcgagaga gccgaggatg gcgtcgcgca gggcggtgct cccgcgggaa
      541 tcgatgcgtt cgagggcttc ttttagttcg ggaatgctgc tcgtgaaatc cttgtcgaga
      601 tcgagataaa agtcatcgtt gaagttgacg acgaaagctt cgtcctgagg gttgcttgcc
      661 tgcacgagag tgatggcggc ttcgttgacg cgagaacgct tgtcgcgcat gctgccgcta
      721 ttgtcgatca ccagccccat gctgacgggg acgtcctcgc gcttgaagac ggagagtttc
      781 tgttcgactt tgtcttcgaa gacgcgaaaa ttttccgact tgaggccgtc ggcgaagcgc
      841 tgacggtcat cgacgacggt cgtgtgcagg acgaccaggt ttacgtcgac cttgatgctg
      901 gaaccttgtt taccggcgtt gggatcctgg ccggggggag gaggcgccga attggggatg
      961 ggaatctgcg cgaaggtggc ggaagcgaag acagcggaag cacagatcaa aagaaaaaaa
     1021 tagcgcaagg aaggaaagag acgccgaaag aactcagtgg ctgggggcgt aatatccggt
     1081 cttggaatag acgctgagcg gcggaagtcc cttgggtgcg cgcaacttga tcttgagctt
     1141 gcgccagcgc gcgtcgtgag ccttgttgct tggtcgatag ccgaggacat attggtttcg
     1201 cagctccatg ccgatttttg aggcgatatc cggcagctca tccagcttct ccacggcaaa
     1261 cacgcggcca ccggtcattt cggttacttc gccgagaagc gacgggccgc tcagctcttc
     1321 cggagaccgg ttgcgatatc caagcgggtc aaagatgccg atggcatata gctgggtgtc
     1381 ggcttctttt accagccgct tgatatcgct ttcgttgtac ctactatgat tatcgcctcc
     1441 atcggatagg atgagcaagg cacgtttggc gttgtgtgca cccctcatct ggctgagacc
     1501 gagatagatg gcgtcgagca gcgcggtgcg gcccttgggg gccgtgagca tcaagcggct
     1561 ctgcaagtct tccacactat tggtgaaaga actggtcaat tcagcgcgct cattgaagct
     1621 gaccagaaag aattcatcct gaggattggc ggtcttgaag aattggacgg cggcttcgcg
     1681 ggccttgcca actttgttgg acatgctgcc gctgaaatca aagatcacgc cgatggagat
     1741 ggggacgtct tcggcggaga agttgacgac ttcctgctcg atgttgtctt cgaagacgcg
     1801 gaagttgtcg ggttcgaggc cggtgaccaa gcggttgtag gggtcggtga cggtaacgtt
     1861 gacgagcgcg aggtcaacgt caaaatgaat cgttcctccg ggcttgaaag gcgtgcccgc
     1921 ggtatctttc cgcacgtcgt cattcttgac ttccttaact tctccgcgtt tggcttcgtc
     1981 cttgggcttg ttttcgtcgg aagtggattg cgcggcggca gggagcacgg cgtgtaacac
     2041 gaacaaacaa acgaccagca agacgtggaa taagaaagct aggacgcgaa aaccaggtaa
     2101 atcgcgtttc acggcacccc cgtcatcccg acctaccgga atggccgcgc ggttcagact
     2161 aacgggaatt ctaccactcg gtttcccgaa aacaaggaca attgcttgat gttcaatatt
     2221 gaacaattgc atgggccggc tggacggagt gccgtgtggt agcatcgccg gacgggaatt
     2281 ccccatgttg aggggagacc gtgcccgagg aacttttatt ccgggcaggc ggagaatgag
     2341 aggagcagag cttggccaag gtggtggtag tcattcccgc gagatacggt tcgacgcggt
     2401 tgccagggaa accgctggta ctgcttgcag gaaagccgat gattcagcgg gtgtacgaga
     2461 gggccaagct ggcgaaacgc gcggaccgag tgattgtggc gaccgacgac gaacgcatcg
     2521 tgaaagcggt ggaggaattt ggcggtgagg ccagaatgac gcggaccgag caccgcaccg
     2581 gaacggaacg cgtggcggaa gtggcggcgc gcgaagaggg ggacgtcttc gtgaacgtgc
     2641 aaggagacga gccgctcttg gatccgaatg cgatcgatgt ggcggtcaat gctttgctgg
     2701 aggagccgca ggcttccatc agcacggtgg cgacgccgat caagacgccc ggcgacatca
     2761 tggacccaaa tgtggtgaag gcggttctgg acttcgacgg gaacgcgatc tacttctcgc
     2821 gcgcgccgat tccctgggta cgggatacgg caagcaaaat ccaagtgcgg catctgaagc
     2881 atcttggact atacgttttc cagcgcgaag ccctgctgga atacccgacg ttgccgcagg
     2941 gcgagctgga acggatcgag caactggagc aactgcgctg gatggaaaac ggctggaaga
     3001 ttcgcgtggc ggaagtggaa cacgacgcgg tgagcgtgga cgttccagaa gatgtggcgc
     3061 gggtggaaaa gctgctacag aaataaatcc gcccggccac atctggccgg gcgggccatt
     3121 gcagttcgtc tcaaatcctc aaggttgtcg cgcaatcgaa cccgcgagga cgcggttgtt
     3181 atccacaccg accagaaaaa ccgtgttcga atccacagcg taagcagtga agttgaacgt
     3241 ggtgggcgac gggaacgtgg ttgtcagcgt caaggtgttt ggaccagtgc ctggggcagt
     3301 aatggtgagc gggccgctga actgaatatt gaagaactgc ttccccgctc cgaactcgtt
     3361 gaaatccatg atgccgctgg cgttattgcc ggaggccgag gtcagcttca actggccgac
     3421 gaagtcctcc tcgtcggtgc tcactccggt ccagacgaag gcgaagtccc ccgccagggt
     3481 cgcgagagag accgggcccg ctgtctgggc caggagtgtg ccgtcgctcg tgatcccgga
     3541 gtcggtctcc tggaaaacgg cctttgtcgg cgagatcaaa tagaaaatga aagagaaggt
     3601 tccgaccttt gtatccgtcc aagttgccgt accgccgccg agtccgttgg catcgacggc
     3661 ataagtcccg gtgactgtgc cgccctgcga cggaaggagc gttagggctc cgttgttgtt
     3721 ttcatcgaga aaaatctcgg aaagattgcc ggcgccgtca gcagtgaaac ggccggcagt
     3781 ggcgatgggc ccacttatgc tttgtttctg gatcgctccg ccaatcagaa aggcaaagcc
     3841 gctgttcagc gaggcggcag taaatgaaat attttgctgc gcaaaggcct ccccggagaa
     3901 tgcagcagga aaatcggtgc cgaggagttt cagccgagtg gcatcgacga tatagaaggc
     3961 aaaattgtgc ccggcgatgt tagcggtgcc gcggccaaag ttagtgccgt cgccgttcgt
     4021 atccagttga tag
//
LOCUS       DASYVO010000530         4496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_154005, whole genome shotgun sequence.
ACCESSION   DASYVO010000530 DASYVO010000000
VERSION     DASYVO010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154005"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VGR03_18265"
     CDS             complement(<1..952)
                     /locus_tag="VGR03_18265"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1996285.1"
                     /translation="MEKLVVIGNGMAGVACVEQILKHAPKFEITIFGNETNVNYNRIL
                     LSSVLAGEKAADEILLNGLEWYERNGIALRLGVRITDVDSERKTVTGDDGSITPFDKL
                     LIATGSTPLIPPMAGVKKDGVFVFRNLDDTRALLNRAAPGKKAVVIGGGLLGLEAARG
                     LQVQGCDVTVVHLMDTLMERQLDLTGGGYLKAKMECLGVKVLLERNTAAILGNGKAEG
                     VAFRDGSSVEADFVVIAAGIRPNVEFGRKAGLQVNRGIVVNDYMETSNPDVFAVGECV
                     EHNGTCYGLVAPLFEQGKVLAATITGNRGAVYGGTIQAAKL"
     gene            complement(999..2255)
                     /locus_tag="VGR03_18270"
     CDS             complement(999..2255)
                     /locus_tag="VGR03_18270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1996286.1"
                     /translation="MNDKKFMESGHFPTLIAAFLYFDVSFMVWVLLGPLAPFLSEMLK
                     LTPYQKGLLTAIPLLGGALFRPVLGFLGERFGERRTGLLGLGLTLIPLIFGWRFAHTV
                     AHFYFLGLLLGIGGASFAVALPIAGRWYPPEYQGLVMGIAGAGNSGTLLATLFAPRLA
                     QKFGWANTFALAMLPVIVVLVLFALLAKDSPRRSEPPKWKSIAAILREPDTAWFCFFY
                     SFTFGGFVGMASFLTTFFHDQYGLSKVRAGDFTTIVVLAGSFLRPLGGWLSDKIGGYR
                     LLLLLLAGVAGCPGGVGRLAALPVEVAFLFLAMGMMGMGNGAVFQLLPQRFPERVGIL
                     TGLVGAAGGLGGFFLPFILGAIKQRTGQYSGGLFLFAGAFFAAALILLQLGTKWRASW
                     QEESAERAGIFSYRGFAETPVGETVE"
     gene            complement(2301..>4496)
                     /locus_tag="VGR03_18275"
     CDS             complement(2301..>4496)
                     /locus_tag="VGR03_18275"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HEV1996287.1"
                     /translation="EEKYAYAYDPVMGYTSEQKIPTHWVSTTCGYCSVGCGMFIGVKD
                     GRAVSVRGDSDHPVNAGKLCPKGLSEHYTLEASNRARYPLLRNKRGKLVRIGWDEALG
                     VMAEKFRGVQDKYGRDTLGVVSTGQLVTEEFYALGKLVQLGLGTRNFDGNTTLCMASA
                     VSGYKRSFGSDGPPGAYEDLTKADVIFLIGANIADNHPILCQYLEANLGMTLIVADPR
                     VTKTAMMADLYLPLKPRSDIALLNGMIHLLIENGLIDRDYIAAHTTGFEQLEESVRKY
                     TPDYVSQLTGLTREQIYKAALIYGRAKAPFIGWTMGVNHSTKGTETVNVINNLALITG
                     NVGKPGASPFSITGQCNAMGTREAGFTSSLPGYRKFESAADREELAALWGIDAERIPT
                     WRGWAYPDIIEGAVAKKIRALWVIATNPVVSFPNLAVLQQSLDGLDFLVVQDGFHPTP
                     TTEIADLVLPAAIWGEKEGTYTNSERRVSKVNRAVNAPGEARADFDIFLDFAKKIGCY
                     DELFSGWTQPKDAFEEWKQISAGRLCDYSGLDYELIERHGGIQWPYPAGATEVKTTRL
                     FTDGHFPTEDGKARLLPVEWEPFPEQPNADFPFILNTGRTVEHWHTRTKTENVPILER
                     LSPNAWLEMNPRDAQSLNLKPHDAVDVVSSRGRVRGVELRVTEIIAPGQVFMPFHYAE
                     ANANQVTQSAFDPISREPNYKQCAVRVEKGQASPQYWGVHGSPRARVQA"
BASE COUNT          986 a         1387 c         1214 g          909 t
ORIGIN      
        1 tcagcttcgc cgcctggatg gtgccgccgt aaaccgctcc gcggttcccg gtaatcgtcg
       61 cggccagaac ttttccttgt tcaaagagcg gcgccaccag gccgtagcat gtgccgttgt
      121 gctccacgca ctcgcccacc gcgaagacat cgggattcga cgtctccata tagtcgttca
      181 ccacgatgcc gcggttgact tgcaatcccg ctttgcgccc gaactccacg ttcggacgga
      241 ttcccgcggc gatgaccaca aaatccgcct ccacgctgct gccatccctg aacgccacgc
      301 cttctgcttt tccattcccg agaatggccg ccgtattgcg ctccagaagg accttcacgc
      361 caagacattc cattttcgct ttgaggtaac cgccacccgt gagatcgagc tggcgttcca
      421 tcagcgtatc catcaagtgg acaaccgtta cgtcgcagcc ttgcacttgc agcccgcgcg
      481 ccgcttcgag tccgagcaga ccgccgccaa tgacaaccgc tttcttgcca ggcgcagcgc
      541 gattgagcag cgcgcgcgtg tcgtccagat tgcgaaagac gaacacgccg tcttttttca
      601 ctcccgccat cggagggata agcggcgtgc ttcccgttgc aatcaaaagt ttgtcaaatg
      661 gcgttatgct gccatcatcg ccggtcacgg ttttgcgttc ggaatccacg tctgtaattc
      721 gaacgccgag acgcaacgca atcccatttc tctcgtacca ctcaaggcca ttcagcagga
      781 tttcatcggc cgccttctcg ccggccagca ccgaggagag caggatgcgg ttgtagttga
      841 cgttggtttc gttaccaaag atggtgattt caaattttgg cgcgtgcttc aggatttgtt
      901 cgacgcaagc gacacctgcc atgccgttcc cgattacgac aagcttttcc atggtcacaa
      961 ctcctcctgg agcaaactct cgcctgcctg tacgcgcctc attcgacagt ttctcccacc
     1021 ggagtttccg cgaaaccccg gtacgagaag ataccggccc gctcagcgga ttcttcttgc
     1081 cagctcgcgc gccatttcgt gccgagctgc aacaagatca aagctgcggc aaaaaaagca
     1141 ccggcgaaaa gaaaaagtcc ccccgaatat tgcccggttc gctgcttgat ggcgccgaga
     1201 ataaaaggca agaaaaatcc tcccaaaccg ccggccgcac cgaccaatcc ggtgagaatc
     1261 ccgacgcgct ccgggaatcg ctgcggcaag agttgaaaaa cggctccatt ccccatgccc
     1321 atcattccca tggcgagaaa caaaaacgcc acctccactg gaagcgcagc gagcctccct
     1381 acaccgcccg ggcaacccgc cacgccagcc aggagcaaca agagcaaccg gtaaccgccg
     1441 atcttgtccg agagccatcc gcccaagggc cgcaggaaac ttcccgcaag gacaacgatg
     1501 gtcgtgaagt cgccggcgcg cacttttgac agtccatact ggtcatggaa gaaggtggtg
     1561 agaaaactgg ccatacccac gaaccccccg aacgtaaagc tatagaagaa acaaaaccaa
     1621 gcggtatcgg gttcccgcaa aatggctgcg atcgatttcc atttgggcgg ttcgctacgc
     1681 cgcgggctgt ccttcgccag cagagcgaag agaaccaaca cgacaatcac gggcagcatg
     1741 gccaaagcga aagtgttcgc ccatccaaat ttttgcgcga ggcgtggcgc gaagagcgtc
     1801 gcgaggagag ttcctgaatt tcctgcacca gcgatgccca tgacgaggcc ctgatattca
     1861 ggcggatacc agcgtcccgc aatcggcaga gccacggcga aactcgcgcc tccgataccc
     1921 agcaggagcc caagaaaata gaagtgcgcg acagtatgtg cgaagcgcca cccgaaaatc
     1981 agcggaatca aagtgagccc gagcccgagc aaaccggtcc tgcgttcgcc aaaacgctcg
     2041 ccgagaaatc ccagtacggg ccggaacagc gctccaccta gaagaggaat cgcggtaagc
     2101 aaccctttct ggtagggcgt gagcttgagc atttcggaca ggaatggagc aagagggccg
     2161 agcaagaccc acaccatgaa actgacatcg aaatagagaa acgccgcgat gagggtcggg
     2221 aagtggccgc tctccatgaa ctttttatcg ttcatctcat ctccttgtac gaaacgaaat
     2281 ttgttccatc cagtccgggt tcatgcctgg accctggctc ttgggctccc atgcacgccc
     2341 caatactgcg gagacgcctg acctttctcg acgcgcacgg cgcactgctt gtaattcggc
     2401 tcacgagaaa tcggatcgaa agcgctctgg gttacctgat tggcattcgc ttccgcgtag
     2461 tgaaatggca tgaagacttg gccgggcgcg atgatctccg taacccgaag ttcgacgccc
     2521 cgcacgcgcc cgcgagaaga caccacatcc acagcatcgt gcggcttcag gttgagcgac
     2581 tgagcgtccc ggggattcat ctccagccaa gcgttcggtg acagacgctc gagaatgggt
     2641 acgttctccg tcttagtccg ggtatgccaa tgctcgacgg tgcgcccagt attcaaaata
     2701 aatggaaaat ccgcattcgg ctgttccgga aaaggttccc actcgaccgg gaggagccgc
     2761 gcttttccat cttccgtcgg gaagtgaccg tcggtgaaga gccgcgttgt cttcacttcc
     2821 gtggcgccgg ccggataggg ccactgaatc ccgccgtgac gttcgatgag ctcgtagtca
     2881 agcccggagt aatcgcagag gcggcccgcc gaaatctgct tccactcctc gaaagcatct
     2941 ttcggctgag tccatccaga aaaaagttca tcgtagcagc cgatcttttt tgcgaaatcc
     3001 aagaaaatgt caaagtcagc gcgggcctct cccggtgcgt taacggcacg gttgaccttg
     3061 ctcacgcggc gttccgaatt cgtgtacgtg ccttcttttt cgccccagat agccgcggga
     3121 aggaccagat cggcgatttc ggttgtcggc gtgggatgaa atccatcctg caccacgaga
     3181 aaatccaaac cgtcgagact ctgctgcaag acggccagat tagggaacga cacgaccgga
     3241 tttgtggcga tcacccatag agcgcgaatt ttcttcgcaa ccgcgccttc gatgatgtcc
     3301 gggtacgccc atcctcgcca agttggtatg cgctccgcgt cgatacccca gagcgctgcc
     3361 aattcctcgc ggtccgccgc actctcgaac ttgcggtaac ccggaaggct tgatgtaaag
     3421 ccagcctcac gcgttcccat cgcattgcat tgcccggtaa tggaaaaagg agacgcccct
     3481 ggctttccga cgttgcccgt gatcagcgca agattattga tgacgtttac ggtttccgtg
     3541 cccttggtgc tgtggttcac tcccatcgtc catccgatga agggagcttt ggcccggccg
     3601 tagatcagcg cggccttgta aatctgctcc cgggtgagcc cggtgagttg cgagacgtag
     3661 tcaggcgtat acttgcgcac cgactcctca agttgctcga agcctgtcgt gtgcgctgcg
     3721 atgtaatcgc gatcgatgag gccgttctcg ataagcaggt gaatcatgcc gttaagaaga
     3781 gcgatgtccg agcgcggctt cagcgggaga taaaggtccg ccatcatcgc ggtcttcgtc
     3841 acgcgcggat cggccacgat caacgtcatg cctaggttgg cctccaggta ttggcaaaga
     3901 atcggatggt tgtcggcgat gttcgcgccg ataagaaaaa tgacgtctgc tttggtgagg
     3961 tcctcgtacg ctcccggcgg cccatcgctg ccaaacgagc gcttgtatcc agacacggcg
     4021 ctggccatgc acagtgtcgt gtttccatca aaattcctgg ttcccagacc aagctggaca
     4081 agcttgccga gcgcatagaa ttcttcggtg acaagctggc cggtactcac cacgccgagc
     4141 gtgtcacgcc cgtatttgtc ctgaacgccg cggaatttct ccgccatcac cccgagcgct
     4201 tcgtcccagc cgatgcgcac gagttttccg cgtttgttgc gtagcagcgg atagcgggcc
     4261 cggttggaag cctccagcgt gtagtgctcg gaaaggccct tggggcaaag ttttccggcg
     4321 ttcaccggat gatcggagtc tccacgcacg ctcaccgcgc ggccatcttt cactccaatg
     4381 aacatcccgc agccaaccga gcaataaccg cacgtcgtcg agacccaatg cgtcgggatt
     4441 ttttgctcgg acgtgtagcc catgacggga tcataggcgt aagcgtactt ttcttc
//
LOCUS       DASYVO010000531        17528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_10663, whole genome shotgun sequence.
ACCESSION   DASYVO010000531 DASYVO010000000
VERSION     DASYVO010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 17528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17528
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10663"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VGR03_18280"
     CDS             complement(<1..341)
                     /locus_tag="VGR03_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996288.1"
                     /translation="MATVIVAVPLLNTPDDPDPGAVNVTFTPETGLLPASFTVTASAL
                     AKAVLIIADCGVVPAFAVIVEGAPTVFVSEKFTVVRPVAAAVTVYGPPVVAFAVNGAE
                     ATPDAFVATVIV"
     gene            445..3192
                     /locus_tag="VGR03_18285"
     CDS             445..3192
                     /locus_tag="VGR03_18285"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEV1996289.1"
                     /translation="MLTNLSASNSIALVQHASKDAGTTTSSTLAFPSSNTAGNFIAVV
                     IRAGHSGQVFTVSDSNKNTYHAAVQFNQTLDAPNGDTFAIFYAENIFGGPNTVTVSDT
                     IAGSTLRFAILEYSGVASFNSLDTTAVAQGGSSSPNSGPATTTMGGELLLGEIMTANS
                     TALSAGAGFKVEEAVPASPSTKLMVEDQIQSVPGSVSATASLGGSFSWGAALAAFKPS
                     GAASGGPNISGISPATGMVTTPVTITGSNFGSSQGLSTATFGGQPAGPASAWSPTSIT
                     VNVPQGAVTGSVVVTVAGIPSNAVSFTVNILAVTLTPTRAAIQISQTQQFTGSVLYDP
                     QNLGLNWYVDTVLGGNTTVGTIDTSGLFSPGTQLGSHTVTAMSVSDATKSASATIAVT
                     DFPGMFTYHNDNSRTGQSFSEYALTPADVNTATFGKLFSCGVDGAVYAQPLWVPNLTI
                     GGAKHNVVFVATAHDSLYAFDADTSPCVTLWQVNLIDALHGGTLGEVTVPSATTGNLV
                     GGGNGDINPEVGVIGTPVIDPATNTLYVVSKSVITSIPSFFQRLHAIDLLTGSEKFGG
                     PANISSAISVPGTGSGSSSGRVAFDPRNENQRAGLALANGNVYISWASHEDHSPWHGW
                     VISFNATSLAFASAFCATPNGNGGGIWMGGGAPAVDSGNNLYVITGNGNWDGVSNFGD
                     SILRLDSALSLSSTAWFTPSDQSSLNSADQDLGSGGAAVLFDLPSAAVPHLMIGGGKQ
                     GSGQLGELYLLNRDSLGGMTSTDTQPQIVQKFAVGKRLFATSTFWNNNLYIAGSSGQL
                     NQYSFNPATEQFTLAQQSSSSFSFPGATAAVSASGNTNGIVWVMNNGSYCAPASCGAT
                     ILHAYDATNISVELWNSTQDATGKDKAGNAVKFTVPTVANGKVYIGTRTEIDVYGLKP
                     N"
     gene            3231..3701
                     /locus_tag="VGR03_18290"
     CDS             3231..3701
                     /locus_tag="VGR03_18290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996290.1"
                     /translation="MAEETVGLVTGIVTGVGVTVNPYIEYFADKERHSRGNLYDPYRE
                     WVRTSKSSGAEQRHGGHGSPEQCRLNVLYEIDQESRRLRCYQQVRASIETTRTQPEVL
                     RDNIPDAPTLDRLLRYEASLERSFDRTLSQLERRQRMRLGQTVLPEIRLHHSRP"
     gene            3882..4016
                     /locus_tag="VGR03_18295"
     CDS             3882..4016
                     /locus_tag="VGR03_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996291.1"
                     /translation="MLYSSPSSGSGAAGPAVKFTVPTVANGKVYVGGQGVLTVFGLLP
                     "
     gene            complement(4264..5790)
                     /locus_tag="VGR03_18300"
     CDS             complement(4264..5790)
                     /locus_tag="VGR03_18300"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HEV1996292.1"
                     /translation="MRAAEGLRRFGIKAGILLVVVSCCFALARLSESGTSGPGTPADK
                     VAGIEKATPLPPPVGRTAATTAYPLKVSTNRRYLVDQNDKPFRIQGDSAQSLISNLTY
                     EEAEAYLSDRQAKGFNSININLLEHKFAVNASANRRGDAPFSAAGNFATPNEAYFAFA
                     DSIIDLAASKGMLVSLAAMYLGYDGRDEGWWAALNNSVNTRTVCYNFGLYIGKRYKDR
                     KNILWVIGGDYLPPTGSEGEARLHKFMEGVKAAGATQLWAGDWNAPCISTDEKAFASA
                     MDVNAVYTLGIPGHTRSTYGEARKAYDYSPTHPAYLKETGYENENWIPGDAASVRMYE
                     YWAILGGSTAGGFFGNRDIWEFATAKWWSSFDFGHSRWQEALDSTGSLDMMRLGQLLD
                     AVPWYNLVPSGQSGMKNLVSEGGGTYGELDYVTAAATADGKVLLAYIPPTAKGRSSVT
                     VDMRQMRGHMRARWFDPTSGKYEEAASYVPKIGRMSFATPGKNSGGATDWILVLQASE
                     "
     gene            complement(6003..9569)
                     /gene="metH"
                     /locus_tag="VGR03_18305"
     CDS             complement(6003..9569)
                     /gene="metH"
                     /locus_tag="VGR03_18305"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEV1996293.1"
                     /translation="MRKLNESARALRELLAQRILVLDGAMGTMLQERNLTAADFGGAA
                     LEGCNENLVRTRPDVVLDIHRKYFEAGSDIVETNSFGGAPIVLAEYGLAADAHFLNKR
                     AAELARQAADEISTPGKPRFVAGSMGPTTKAITVTGGVTFDELRETYYTQARGLVEGG
                     ADLLLIETCQDTRNIKAALLAILKLSKEMGSEVPFIVSVTIEPMGTMLAGQTVEAMWA
                     SLRHAKPLAFGMNCATGPEFMTDHIRTLSQITGEFVSCYPNAGLPDEEGKYLETPTTL
                     AGQLEKFADHGWLNFVGGCCGTTEKHIRAIAQMVEGKKPRQRPAEAHRAVYSGIETIE
                     AEDSTRPLLVGERTNVIGSRLFKNLVAEEKWEEASEIARRQVRGGAHIVDVCLQSTER
                     DEKNDIPPFYEKLIRKVKAPVMIDTTDPTAIELALTYCQGKAILNSINLEDGEEKFER
                     VVPMAHEFGAAVVVGCIDEDKLQAQAFTRERKLATAQRSYQLLTEKYGLGPEDIIFDP
                     LVFPCATGDTNYIGGAVETMEGIRLIKQALPDVRTILGISNVSFGLPAGAREVVNSVF
                     LYYCTKAGLDLAIVNTEKLERFASIPAHERELAENLLFSHPPKDVPADHSNAALLRDV
                     PADWRKQSKEQRAAVNQHYIAAIAEHFRTTKKKEKARAADVPLDERLSNYIIEGTRDG
                     LIADLERKRGEGAAPLDIVNGPLMAGMAEVGRLFNANELIVAEVLQSAEAMKAAVSHL
                     EQFMEKADTAKRGKVVLATVKGDVHDIGKNLVEIILKNNGYDVVNLGIKVPPEELIKA
                     YQEHKPDAIGLSGLLVKSAQQMVITANDLKDAGIEVPMLVGGAALSAKFTQTKIAPSY
                     GKAVCYAKDAMTGLRLMNELMDPATRETVLRNHTATGNGASVATIVTVQEIPAVRRSP
                     RVRADLPIPAVKDLDRKVRLVPDLREVWGYINPFMLFGRHMGFRGDFEKRLAEREPKA
                     VELFENMEEVKKEAAAFMKIRAVWQFFEAEAEGDSLHLFAPGAMAPLHTFRFGRQRQG
                     DFLCLADYVLPPKNGQRDHLALFVVTAGEGIRSRSEEAKNEGYYFKSHGLQALAIETA
                     EACAEWLHRRIREDWGFPDPPEMTMAQRFTSRYRGKRYSFGYPACPNLEDQAGIWKLL
                     KPEEIGVQLTEGMMMDPEASVSALVFHHPACPYFSVGDANDT"
     gene            9792..10628
                     /gene="wecB"
                     /locus_tag="VGR03_18310"
     CDS             9792..10628
                     /gene="wecB"
                     /locus_tag="VGR03_18310"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEV1996294.1"
                     /translation="MSQRLKILNIVGARPNLPKIAPLMREMQRHPEIEAVLVHTGQHY
                     DEKLSDVFFRQMGIPAPHLNLEVGSGSHASQTAEILKHIEPVLVERQPDLVLVVGDVN
                     STIAVSLAAAKLGIRVAHVEAGLRSFDRSMPEEINRILTDALADYLFVTEEDAIAHLL
                     REGRPRESIFMVGNVMIDSLRHFLPVAQQSGIGDELGLKNGLGWHRFGVLALHRPSNV
                     DSTEKLSQLLGAIDAVAAEMPVIFPVHPRTQQRLTQGGIQHHPQLRLIPSPSGTSIFS
                     VS"
     gene            10991..11665
                     /locus_tag="VGR03_18315"
     CDS             10991..11665
                     /locus_tag="VGR03_18315"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1996295.1"
                     /translation="MTTKRASTKLTVCILSPHPLVLSEFERILAKSQFKVIAKQLEST
                     LAPDLRAFEPPRAQVYVIDAHAAKPATGALLANLLERYPEARLIVVGEQHNDANSFSL
                     LRLGVKGLLTYVEARDQLIRALPLVASGGFWVPRQLLSGFVDSILTSQGRRLKTDSVT
                     NLSRREQEVLDSLLENLSNKEIGSKLNIAERTVKFHVSNLLNKFGVRRRADLILLTFQ
                     RRLAQA"
     gene            11782..12774
                     /gene="galE"
                     /locus_tag="VGR03_18320"
     CDS             11782..12774
                     /gene="galE"
                     /locus_tag="VGR03_18320"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1996296.1"
                     /translation="MNILVTGAAGYIGSICSEVLVSRGMSVIALDSLLEGHRAAVPPD
                     AVFCQVDLADRPQLESVFTQHKIDAVMHFAAEALVAKSVSEPSIFYATNVACGVNLLD
                     AVTRHGVKKFIFSSTAATYGEPEIVPIPEDHRKAPINPYGKSKLVFEQILADYKVYTG
                     LKYVTLRYFNAAGASKERGEHHRVETHLIPRVLDAASGGLPHVDVFGTDYPTPDGTCV
                     RDYIHVLDIADSHVRALEEIDKATGEAFNVGNSRGFSILEVIDAAERITGRKIPRKLG
                     PRRPGDPAVLVASKEKLKRILGWEASHSSLEEIIQSAWAWKQKHPQGYTDAASV"
     gene            complement(12778..13557)
                     /locus_tag="VGR03_18325"
     CDS             complement(12778..13557)
                     /locus_tag="VGR03_18325"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1996297.1"
                     /translation="MAHQRQNPSALHDAKVSIVIPVYNEKSTIDEILRRVLDTEVRKE
                     VIIVDDCSTDGTRQILENMAARQANGEASAAAQDGGDQIELRDLRFFFQAPNQGKGAA
                     LRRGFAQVTGEIVLVQDADLEYDPRDYGVLLEPIVEGRADVVYGSRFLSGPQRVHYFW
                     HYVANRMLTLLSDIFTNLKLTDMETCYKVFRREVLAGVQVKSNRFGFEPEITAKIAKH
                     KWRIYEVPITYAGRTYEEGKKITWRDGIQALWCIIRYKFTD"
     gene            13810..15258
                     /gene="mtaB"
                     /locus_tag="VGR03_18330"
     CDS             13810..15258
                     /gene="mtaB"
                     /locus_tag="VGR03_18330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEV1996298.1"
                     /translation="MPSFFIEQFGCRATQADGAALERQLLDRGCTSASTAAADIIVVN
                     TCTVTASADAQARDAIRKLHAANPAVRVIVTGCYAQRAPEELAALPGVSWVVGNSHKP
                     QIPQLVDSLTTPASPESANNFFPAAALHSDSTVPFHSFVRAQHAAPQLGKILTGDICD
                     RTEFLSAPVLGGEGNHTRPTLKIQDGCDSRCSYCVIPFVRGKSRSLPPNTVIQEIQRL
                     AEAEYREIVLSGINLGTYGRDLSPRVEFEILLRRILDETSVERLRISSIEPLDVTQDL
                     VALFASTDRIAQHFHMPLQSASDRILAAMHRWYRAEHYARRVELIHERLPNAAIGADV
                     ITGFPGESEDDHAATIAFIERLPFTYLHVFSFSKRPGTKAASLANEVTGSTIKRRARE
                     LRALSEKKSAAFRHSQLGRTLRVLTLRPSDDPRSNTTPALSSNYLRVRIVGALSANLW
                     VDVQVTGEEEMFLIGTGICDGQGQHARIPANV"
     gene            complement(15606..15983)
                     /gene="acpS"
                     /locus_tag="VGR03_18335"
     CDS             complement(15606..15983)
                     /gene="acpS"
                     /locus_tag="VGR03_18335"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV1996299.1"
                     /translation="MIIGMGVDIAEVDRIQAAIERHGEVFLRRIYTPREREYCERFKN
                     KYERYAGRFAAKEAAMKALGTGWRRGVKWLDLEVMRETSGRPTLALTGEAAKIAKQLG
                     VNSVALSITHTAAQALAQVIFES"
     gene            15918..17000
                     /gene="pgeF"
                     /locus_tag="VGR03_18340"
     CDS             15918..17000
                     /gene="pgeF"
                     /locus_tag="VGR03_18340"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEV1996300.1"
                     /translation="MALNRRLDAVHLSNIHAHSNDQDASSAPAFGPAAHSDYTVRVVA
                     PTKTLKPKRKVRERPAVGQTCLSVSFGRSNSNWVERKVRGVQILQLPTLTKIPWLVHG
                     FSTRPGGVSLMDKEKVLNLGFTEWDTKENVLENCLRFQSALGASNLKLISLKQIHSDV
                     IHLFDAAPAQPCQGDASSTNRPGLLLGVQTADCVPILLVDPQKRAIAAVHAGWRGTLQ
                     RIVVKAIGQMQMQFKSKPADLLAAIGPAIGGCCYEVGTEVAIQFQSQFADAHEWFDEF
                     RTGDEPNPIQWLNMMPPGHQPPPKNVLLDLRKANRAQLLGAGLRAPNVFVSDLCTACR
                     RDLLFSYRKEGALSGRLMSVIGIQPS"
     gene            17181..>17528
                     /locus_tag="VGR03_18345"
     CDS             17181..>17528
                     /locus_tag="VGR03_18345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996301.1"
                     /translation="MSTNRSKDRSRLCAFTFADGRRCRTPRCAGHPHLCAFHAQKEAQ
                     SRAAAQAGEDVSYFLSGTYLSACDLTSALGRLFSAVAQGQVKPKTATALAYLGQTLNH
                     SIQLAQHEFINAYG"
BASE COUNT         3491 a         5726 c         4468 g         3843 t
ORIGIN      
        1 acgatcactg tagccacgaa cgcatcgggt gtagcttcgg caccgttcac ggcaaacgcg
       61 acgacgggag ggccgtacac agtcacggcg gcggcaaccg gtctgacaac ggtgaacttc
      121 tcgctgacga acacggtggg ggcaccttca acgatgacgg cgaatgcggg aacgacgccg
      181 caatcggcga tgatcagcac cgcctttgcc aacgcgctgg cagtgacggt gaaggatgcc
      241 gggagcaatc cggtttccgg agtgaacgta acgttcacag cgcccgggtc gggatcgtca
      301 ggggtattta gcaacggcac ggcgactatc actgtagcca cgaacgcatc gggtgtagct
      361 tcggcaccgt tcacggcgaa cacgacagcg ggagggccgt atacggtcac agcggcagcg
      421 acaggcctga caacggtgaa cttcttgctg acgaaccttt ccgcgagtaa ctcaatcgcg
      481 ctggttcaac acgccagcaa ggatgcgggt accacgacgt catctactct cgcttttccc
      541 tccagcaata ccgcgggaaa ttttatcgcc gtggtgatcc gcgccgggca ttccggtcag
      601 gtgtttactg tcagcgacag caacaaaaat acataccacg ctgccgtgca atttaatcag
      661 actctggacg ccccgaatgg agacaccttc gccattttct acgcggagaa catattcggt
      721 gggcccaata ccgtaacggt ttccgacaca attgctggca gcaccctccg attcgcgatt
      781 ttggagtaca gcggggtagc ttcgttcaac tctctggata cgacggcggt agcgcagggc
      841 gggagtagct caccgaatag cgggcctgcg accaccacaa tgggaggaga gctgctgctg
      901 ggcgagatca tgaccgcgaa cagtacagca ctgagcgctg gtgctggttt caaggttgaa
      961 gaggctgtgc cggcttcccc aagcaccaaa ctaatggtgg aggaccaaat tcaatccgtc
     1021 ccaggttcgg tgtcggcaac cgcctccttg ggaggctcgt ttagctgggg cgcggcactt
     1081 gctgcgttca agccgagcgg ggcggcaagt ggggggccaa atataagcgg catttcgcct
     1141 gctaccggta tggttactac gcccgtgacc attacaggaa gcaatttcgg ctcgtctcaa
     1201 ggcttgagca cggcgacttt cggcggacag ccggctgggc ccgcgagtgc atggagccca
     1261 accagcatca cagtgaacgt gccgcaaggg gcagtgacgg gcagtgttgt ggtgacggtg
     1321 gcggggatac cgagcaacgc ggttagtttc acggtgaata tacttgccgt aacactgact
     1381 ccgactcgcg ccgccatcca gatttctcaa acgcagcagt ttaccggcag cgttttgtat
     1441 gatccgcaga acctggggtt gaattggtac gtggacacgg tgcttggggg aaacacgacg
     1501 gtcggcacga tcgacacctc cggtcttttc agcccgggta cccagctggg aagccatacg
     1561 gtgacggcga tgagcgtatc ggatgccact aagagcgcca gcgcgaccat cgcggtgacc
     1621 gatttcccgg gaatgtttac gtatcacaat gacaattccc ggacgggaca aagcttcagc
     1681 gaatatgcgt taacgccggc agatgtgaat acagctacgt ttggcaagct gttcagttgc
     1741 ggcgtcgatg gtgctgtgta cgcgcagccg ctgtgggtgc cgaatctgac gattggcggg
     1801 gccaaacaca acgtagtttt cgtagcaacg gcacacgata gtttgtacgc ctttgacgcg
     1861 gacacaagtc catgcgtgac gttgtggcag gtgaacctga tcgacgcgct gcacggcggg
     1921 accctggggg aggtgacggt gccttcggct acgaccggca acctggtcgg cggtggcaac
     1981 ggcgacatta atcctgaagt tggagtcatc ggtactccgg tgatcgatcc cgcgactaac
     2041 acgctttatg tagtcagcaa gtcggtgatc acgagcatcc ccagtttttt ccagcgctta
     2101 cacgcaatcg acctcttgac gggcagcgag aaattcgggg gcccggcgaa tatttcttca
     2161 gcgatatctg ttcctgggac tggcagcggc tcttcctcag gccgcgtcgc gtttgacccg
     2221 agaaatgaga atcaacgggc agggctggcg ctggcgaacg gaaacgtgta tatcagctgg
     2281 gcgtcgcacg aggaccattc cccatggcat ggatgggtga ttagtttcaa tgccaccagc
     2341 ctggcatttg cttcagcatt ttgcgccacg cccaacggca acggcggcgg aatctggatg
     2401 ggcggcggcg cccccgcggt tgatagcggc aacaatcttt acgtgattac gggaaacggc
     2461 aattgggatg gggtgtccaa cttcggagac agcattctaa ggctggacag cgcgctttca
     2521 ctctcgagca cggcctggtt cacgccctcg gaccaaagct cactgaacag cgccgatcag
     2581 gatctgggct ccggcggggc tgctgtgctg tttgatcttc cttccgcggc tgtgccacac
     2641 ctgatgattg ggggaggcaa acagggcagc ggacaactcg gtgaactgta cctgctcaac
     2701 cgcgattcgc tgggtggaat gaccagcacg gatacgcaac cgcaaattgt acagaagttt
     2761 gcagtcggca agcgcctatt cgccacctct acgttctgga ataacaatct ttatatcgca
     2821 ggatccagcg gccagctgaa ccaatacagc ttcaatccgg cgacggaaca atttacgctt
     2881 gcccagcagt ccagtagttc gttcagcttt ccgggagcca cggcggcggt ctcggcgtca
     2941 ggaaacacaa atggaatcgt ctgggttatg aacaacgggt catactgcgc gccggccagt
     3001 tgcggtgcca cgatacttca cgcgtacgac gcgactaaca tcagcgttga gctttggaac
     3061 agtacgcaag acgcgaccgg caaggacaaa gctggtaatg ccgtcaagtt tactgtgccg
     3121 acggtagcga acggcaaggt ttacattggg actaggactg agattgatgt atatggcttg
     3181 aagccgaact gacgaccgta tgatgggctt actcggaaag actagcgtcc atggcggagg
     3241 aaactgtcgg tttggtgaca ggcattgtca ccggggtggg agtgaccgtg aatccttaca
     3301 tcgaatactt cgccgacaag gaaaggcatt cgcgcgggaa cctctacgac ccctatcgcg
     3361 agtgggtgcg tacttcaaag agttctggag cagaacagcg gcacggagga catggatccc
     3421 cagagcagtg tagattgaat gtcttgtatg agatcgacca ggaatctcgt cgcctaaggt
     3481 gttaccaaca agtacgcgcc tctatcgaga ccacgcgaac tcagcctgaa gttctgcgtg
     3541 acaatatacc tgatgccccg acgctagatc gcctgctgcg ctatgaagcg agcctcgagc
     3601 ggtctttcga ccgaacgcta agtcagctcg aacgccggca gcggatgcgg ctgggccaaa
     3661 cggtcctgcc agaaatcagg ctgcaccatt cgcgccccta aggttagctg atgggaatga
     3721 gctgggaaaa cgcgctccag cacaccttta gtggaccgcg ctaagccggt atctcagcct
     3781 gcggcaccac caacggtgtc gtatggattc tggatctttc tggctggacc ggcaccgcga
     3841 gtgccgtcct ctatgcttac gacgccacca acgtcgcgaa catgctctac agcagcccct
     3901 ccagcggctc aggtgccgcg ggtcctgctg ttaaattcac cgtacccacg gtggccaacg
     3961 gaaaagtcta cgtgggaggc cagggcgtgc tcaccgtgtt cggtttgctc ccctaagtgg
     4021 gaagagccct cgcagctggg ctctttgagg cagggtgggc cggccgattc aggtccgcca
     4081 cgcgcagcgg tggctgccat cacttgcggc tggatttgca ctcccattcc acgttcgggc
     4141 tgaagggcac caagtgattc gaatccgccc acgaactact ccatggttgt gttgtgcgag
     4201 acaagactcg gttcagaagt ttgcccaagc cgccgtagcc agaccgttcg attacatccc
     4261 ctctcactcg ctcgcctgaa gaacaagaat ccaatctgtc gcgcctcccg agttcttccc
     4321 cggtgtcgca aagctcatcc ttccgatctt agggacgtaa ctcgctgcct cttcgtactt
     4381 tccagacgtc gggtcgaacc agcgcgcgcg catgtgaccg cgcatctgtc tcatatccac
     4441 agtcacgctg gatcgtcctt tcgcagtcgg aggaatgtac gcgagtagca ccttaccgtc
     4501 ggctgtcgcc gctgccgtaa cgtaatcaag ttctccgtag gtgccgcctc cctcactcac
     4561 aagattcttc atcccactct gtccggaagg aaccaggttg taccacggca ccgcatccag
     4621 cagttggccc agccgcatca tatccagcga tcccgtggag tccaacgcct cttgccaccg
     4681 gctatggccg aagtcgaaac ttgaccacca ctttgccgtc gcaaactccc agatatcacg
     4741 atttccgaag aacccgcctg ctgtcgaccc tcccaggatc gcccagtatt cgtacatgcg
     4801 cacggaagcc gcatccccag gtatccaatt ctcgttctcg tagcctgttt ccttcaggta
     4861 ggccgggtgc gttggcgaat aatcgtaagc cttgcgcgct tcgccgtaag tgctgcgggt
     4921 atgccccggt atgcccaacg tatagaccgc gttaacgtcc atagcggatg cgaacgcctt
     4981 ttcgtcggtc gagatgcacg gggcattcca gtcgccggcc cataactgag tggcgccggc
     5041 cgccttcacg ccttccatga acttgtgaag tcgcgcttct ccttccgagc ctgtgggcgg
     5101 tagataatcc cccccaatca cccacagtat gttcttccga tccttgtatc gttttccgat
     5161 gtacaagcca aagttgtaac aaaccgttcg agtattgacg ctgttattca gcgctgccca
     5221 ccacccctcg tctcttccgt cgtagccgag atacattgcc gcaagactga cgagcatccc
     5281 tttcgaggcc gccaaatcga tgatcgaatc cgcaaacgca aaataggctt cgttgggcgt
     5341 cgcgaaattc cccgctgccg agaacggtgc gtcacccctc ctgttggctg aggcgttgac
     5401 tgcgaatttg tgctcgagca gattgatgtt aatgctgttg aatcccttgg cctgcctgtc
     5461 cgagaggtaa gcttcggctt cctcgtaagt gagattgctg atgagcgatt gtgcggagtc
     5521 cccttgaatt cggaatggtt tgtcattttg atccacaagg tacctgcggt tcgtcgatac
     5581 cttgagcgga taggcggttg tagccgccgt ccgcccgacc ggtggaggca gaggtgttgc
     5641 cttctcaatg ccggccacct tgtctgccgg tgtcccgggt ccggatgtcc ctgattcact
     5701 taaacgcgcc agcgcgaaac aacaggacac caccaccaaa agaataccgg ccttaatacc
     5761 gaacctcctc agtccttcgg ctgccctcat cgttttcttc agccccttct tccggggtct
     5821 gcaccccgtt tctttcaaga gattctcgga agagagcatg tttcgaatcc cgagttcact
     5881 atccacattg tgcacgctag gaaaggcgct gggaagactg tgatcgatcc gggcatggat
     5941 ctctttgtcg ggccgctgag accaaactcg atggcgcggc gagggagtgg attgccgcga
     6001 agctatgtgt cgttcgcgtc gcccacagag aagtatgggc aggccggatg atgaaatacc
     6061 agcgcgctca cgctcgcttc tggatccatc atcattccct cggtaagttg cacgccgatc
     6121 tcttccggct tcagtagttt ccaaattccc gcctgatctt cgagattcgg gcacgccgga
     6181 tacccaaagc tgtaccgctt cccgcggtag cgcgaagtaa atcgttgcgc catagtcatc
     6241 tccggcggat cggggaatcc ccaatcctcg cgaatccgcc ggtgcagcca ttccgcacac
     6301 gcttccgccg tctcgattgc cagcgcctgc aggccgtggg atttgaagta atatccttca
     6361 tttttcgctt cttccgagcg gctgcgaatg ccttcgccgg ccgtcactac aaacagcgcc
     6421 aggtggtcgc gctgcccatt cttcggcggc aagacataat cggctaagca cagaaaatca
     6481 ccctgccgtt gcctcccaaa tcgaaacgta tgcagcggtg ccattgctcc cggcgcaaac
     6541 aggtgcaggg aatctccttc cgcttccgcc tcgaagaatt gccaaacggc tcggatcttc
     6601 atgaacgccg cggcttcctt cttcacctct tccatgttct cgaatagctc gacggccttc
     6661 ggctcgcgtt ccgctaatcg cttctcgaag tcccctcgaa aacccatgtg ccgcccgaac
     6721 agcatgaacg ggttgatgta gccccagact tcgcgcaagt ccggcaccag ccggactttg
     6781 cggtccagat ctttcaccgc cggaatcggc aggtccgcgc gcacacgcgg cgagcgcctc
     6841 accgccggga tctcctgcac tgtcacgatc gtcgccacgc tcgcgccatt tcccgttgcc
     6901 gtgtgattcc gcaaaacggt ctcgcgcgtc gctggatcca tcaattcgtt catcagccgc
     6961 agcccggtca tcgcgtcttt cgcgtagcac accgctttcc cgtagcttgg cgcgatcttc
     7021 gtctgcgtaa actttgccga aagcgccgcg ccgcccacca gcatgggaac ctcgatcccc
     7081 gcatccttca agtcgttcgc ggtaattacc atctgctgcg cgctcttcac cagcagcccg
     7141 gacaatccaa tcgcgtccgg tttatgctct tggtaagcct tgatcaactc ctccggcggc
     7201 accttgatcc caaggttcac cacgtcatag ccattatttt tcaaaatgat ctcaacgagg
     7261 tttttcccaa tgtcgtgaac gtcgcccttc acggttgcca gcaccacttt tccgcgcttg
     7321 gccgtgtccg ccttctccat gaactgctcg agatggctca ccgctgcttt catggcttcc
     7381 gcggactgca acacttccgc gacgatcaat tcattcgcat tgaaaagccg cccgacttcc
     7441 gccatgcccg ccatcagcgg cccgttcacg atatccagcg gcgccgcgcc ttccccgcgc
     7501 tttctctcca gatctgcgat caggccgtct ctcgtgcctt cgataatgta gttcgaaagc
     7561 cgctcatcca gtggcacgtc cgccgcgcga gctttttcct tcttcttcgt cgtgcggaag
     7621 tgttcggcaa tggctgcgat gtagtgctga ttgaccgccg cgcgctgttc cttgctctgc
     7681 ttgcgccagt cggccgggac gtcccgtagc aacgcggcat tggaatgatc ggctggcaca
     7741 tctttcggcg gatgtgaaaa cagaaggttt tccgcgagct ctcgctcatg cgccgggatc
     7801 gacgcaaacc gctccagctt ctccgtattg acgatcgcga ggtccagccc cgccttcgtg
     7861 cagtaataca agaaaaccga gttaaccact tcgcgcgccc ccgccggcag cccgaacgaa
     7921 acattggaaa tacccaggat cgtccgaaca tccggcagag cctgcttgat cagccggatg
     7981 ccttccatcg tctcgaccgc gccgccgata tagttcgtat cacccgtcgc gcaggggaac
     8041 accagcggat cgaatatgat gtcctccggc cccagcccgt atttctccgt cagcagctgg
     8101 tacgaacgct gcgcggtcgc cagcttccgc tcgcgcgtaa acgcttgcgc ctgcagtttg
     8161 tcctcatcga tgcagcccac cacgaccgcc gcgccgaact catgcgccat gggcaccacg
     8221 cgctcaaatt tttcttcgcc gtcctccaag ttgatcgaat tgaggatcgc tttgccctgg
     8281 caataggtca gcgccagctc gatcgccgtc ggatcggtgg tatcgatcat caccggcgcc
     8341 ttcacttttc ggatcagctt ttcgtagaac ggcggaatat cgttcttctc atcgcgctcc
     8401 gtgctctgca agcaaacatc cacgatatgc gcgccgcctc gcacctgacg ccgcgcaatc
     8461 tcgctcgctt cttcccactt ttcctccgcc accaggtttt tgaacaatcg cgagccgatc
     8521 acgttcgtgc gctcgccgac gagcagcggc ctcgtgctgt cctccgcctc gatcgtttca
     8581 atccccgaat aaaccgcccg gtgcgcctcc gcaggccgct gccgcggttt cttaccctcc
     8641 accatctgcg caatcgcccg aatatgtttt tccgttgtgc cgcagcatcc cccgacgaaa
     8701 ttcaaccagc cgtgatccgc gaatttctca agctgtcccg ccagcgtcgt cggagtttcc
     8761 aggtactttc cctcttcatc cggcagaccc gcattcggat agcaggacac aaattcgcct
     8821 gtaatttggc tcagagttcg gatgtggtcg gtcatgaact ccggccccgt tgcgcagttc
     8881 atcccaaacg ccagcggctt tgcatgccgc agcgatgccc acattgcctc caccgtctgc
     8941 ccggccagca tcgttcccat cggctcgatc gttaccgata cgatgaaagg cacttcgctc
     9001 cccatctctt tcgaaagctt gagaatggcg aggagcgccg cctttatatt tcgggtgtct
     9061 tggcaggttt cgatcagcag cagatccgct ccgccctcca cgaggcctct tgcctgtgtg
     9121 taataggtct cgcgaagttc gtcgaacgtc accccgcccg taacagtaat cgccttcgtt
     9181 gttggcccca tggagccggc tacgaagcgt ggcttcccag gagtactgat ttcatccgct
     9241 gcttgtctcg ccagttctgc cgcgcgcttg ttcaggaagt gcgcgtccgc ggccaggccg
     9301 tactccgcca gcacaatcgg cgctccgccg aagctgttgg tttcgacgat gtccgatccc
     9361 gcctcaaagt atttcctgtg aatatccaga accacatccg gccgcgttcg aaccaggttt
     9421 tcattgcacc cttcgagggc agctccgcca aagtccgccg cagtcaggtt ccgttcttgc
     9481 agcatcgtcc ccatcgcgcc gtccagcacg agtatccgtt gcgcaagcaa ctcacgtaag
     9541 gctctggccg attcgttcaa tttacgcatt cgctggagag tcttactcct tcccggtgcg
     9601 ccggacaaaa tgctacaagt accaaggaaa tctggcgttt ccggacatct ccattttacc
     9661 caggttcccc cgcggatacc tatctccacg cattgaacta gtgctttttt cgcgcgaatg
     9721 gcaaatcggt cgcaccgaaa gcccaattcc cggatgctat actgcctccg gacgccggaa
     9781 acgaacgctc catgagccaa cgcctgaaaa tcctgaatat cgtcggggcg cgcccgaacc
     9841 tcccaaaaat cgccccgctc atgcgcgaga tgcagcgcca tccggaaatc gaggccgtcc
     9901 tcgttcacac cggccagcac tatgacgaaa agctgtccga cgttttcttc cgccagatgg
     9961 gcatccccgc accgcacttg aatctggaag ttggctccgg ctcccacgcc tcgcaaaccg
    10021 cggaaattct gaaacacatt gagccggttc ttgtcgaacg ccagccggat ctggttctcg
    10081 ttgtcggcga cgtcaattcc accattgctg tttcgctcgc cgccgcgaaa cttggcatcc
    10141 gcgtagctca cgtcgaagcc gggctccgta gctttgaccg ctctatgccc gaggagatca
    10201 accgcattct caccgacgcg ctcgccgact atctctttgt taccgaagaa gacgccatcg
    10261 cgcacctcct gagagaaggc cggccccggg aaagcatctt catggtcggc aacgtgatga
    10321 tcgattcctt gcgccacttt ttgcccgtcg cccagcagtc cggtatcggt gatgagctcg
    10381 gattgaagaa tggattgggc tggcatcggt tcggtgtcct ggccctccat cgaccttcca
    10441 atgtcgattc cacggagaag cttagccagc ttctgggagc gattgatgcc gttgccgccg
    10501 aaatgcccgt catctttccg gtgcacccgc gcacccagca gcgtttgacg caaggcggca
    10561 tccaacatca ccctcagctg cggctcattc catccccgtc gggtacctcg attttctctg
    10621 tctcctgagc aaagcaaagc tcgtccttac cgattctggc ggcatccagg aagagaccac
    10681 cgctctcggt gtcccctgcc tcacgctacg ggagaacacc gagcgtccca tcaccatctc
    10741 ccaaggcaca aacctgctgg ttggtactga tccggacaag atcgtcgccg ccgcccgtga
    10801 tacgctggca ggcaagggca aagccggccg cattcctcct ctctgggatg gccatgctgc
    10861 caagcgcatc gttgatattc tattgaggga agtaccccgg ggacatgcat cgtagctgtc
    10921 agtaacagtt cgtgaacaca aacaccaaag ggcttgcccc tttagcccgt tttcaatact
    10981 ctgtcgaaca gtgactacga aacgcgcctc tactaaactg acggtttgca tcctctcgcc
    11041 gcaccccctg gtgctgagcg agttcgaacg cattttagca aagtcccaat tcaaggttat
    11101 tgccaagcaa ctggaatcca ctctcgcacc ggacctccgt gcttttgagc cgcctcgcgc
    11161 tcaggtttac gtcattgacg ctcatgccgc aaagcccgcc accggcgcgc tcttggccaa
    11221 tcttctcgaa cgctatcccg aggcccgcct catcgtcgtc ggcgagcagc acaacgatgc
    11281 caacagtttt tcgctcctgc gtctgggcgt gaagggcctt ctcacctacg ttgaagcccg
    11341 cgaccaattg atccgtgctc tcccgctcgt tgccagtgga ggattctggg ttccgcggca
    11401 gctcctctcc ggattcgttg attccattct caccagccag ggccgccgtc tgaagacgga
    11461 ttccgtcacg aatctcagtc gccgcgaaca ggaagttctc gattcgctgc tggagaatct
    11521 ttccaacaaa gagattggca gcaagctcaa cattgcggaa cgcactgtga agtttcacgt
    11581 ttcgaatctg ctcaataagt tcggcgtgcg ccgccgtgcc gacctcattc ttcttacctt
    11641 tcagcgtcgc ttggcgcagg cttaatcttt gtcgtatttt cgggccggtg tacccttctt
    11701 ttccgccggc catgctgttg gttcaggcaa ttttgagcgc acccattttg cgtttctaac
    11761 tgacttgagg agaactgaca ggtgaatatt ctcgttaccg gagcggcggg ttacatcggc
    11821 agcatctgtt cggaggtatt ggtttcgcgc ggcatgtccg tcatcgcctt ggatagcttg
    11881 ctggagggcc atcgtgccgc cgtgccgccc gacgccgtct tttgccaggt agatctagcg
    11941 gatcgccctc aacttgaaag tgtcttcacg cagcacaaga ttgacgccgt gatgcacttt
    12001 gccgccgaag ccctggttgc caaatccgtt agcgaaccca gtattttcta cgccaccaac
    12061 gtagcctgcg gcgtgaacct gctcgatgcc gtcacccgcc acggcgtaaa gaaattcatt
    12121 ttttcttcca ctgccgctac ctacggcgag ccagaaatcg tccccattcc cgaagaccat
    12181 cgaaaagcgc ctatcaaccc ctacggaaaa tccaagctcg tattcgaaca gattctggcg
    12241 gactacaagg tctacaccgg actcaaatac gtgactctcc gctatttcaa cgctgccgga
    12301 gcttccaagg aacgtggcga gcatcatcgc gtggaaactc atctcattcc tcgggttctc
    12361 gatgccgcca gcggtggcct tccccatgtc gatgtcttcg gcacggacta ccccacaccg
    12421 gacggaacct gcgtccgcga ttacatccac gtgctcgata tcgccgattc ccacgtacgc
    12481 gcgcttgagg aaatcgacaa ggctaccggc gaagccttca acgtcggcaa cagccgcggc
    12541 ttctctattt tggaagtcat cgatgcggcg gagcgcatca ccggccgcaa gatccctcgc
    12601 aaactgggtc cgcgccgtcc cggtgatccc gctgttctcg tcgccagcaa ggaaaaactc
    12661 aagcgcattc tcggctggga agcctcccat tcctccctcg aagaaatcat tcagtcagcc
    12721 tgggcgtgga aacagaagca tccccagggc tatacggacg cggcttccgt ttagaattta
    12781 atcggtgaat ttatagcgga tgatgcacca caacgcctgg atgccgtctc tccaggtaat
    12841 cttcttgcct tcttcgtagg tgcgccccgc ataagtaatc ggcacttcgt aaatccgcca
    12901 cttgtgtttt gcgatttttg ccgtgatctc cggttcgaat ccaaaccggt tcgacttgac
    12961 ctggaccccc gccagtactt ctctccggaa caccttgtaa cacgtctcca tatccgtcag
    13021 cttcaggttc gtgaagatat ccgaaagcag cgtcagcatc cggttcgcaa cgtagtgcca
    13081 gaagtaatgc acgcgctgcg gtccgctgag aaatcgcgac ccatacacca cgtcggcgcg
    13141 gccctcgacg atcggctcca gcaaaacccc ataatcgcgc ggatcgtatt ccaggtccgc
    13201 atcctgcacc agcacgattt ctccggttac ttgcgcgaaa ccgcgccgca aagccgctcc
    13261 cttgccctgg ttcggcgcct gaaagaaaaa cctcaaatcc cgcaactcga tctggtcgcc
    13321 cccatcctgc gcagcagcgg atgcttcgcc attcgcttgc cgcgccgcca tgttttccag
    13381 aatctgccgc gtcccgtcgg tcgaacaatc atcgacgatg atcacttcct tgcgtacctc
    13441 agtgtccagc actcgccgca ggatttcatc gatcgtcgat ttctcgttgt acacgggaat
    13501 gactatcgag actttcgcat cgtgcagcgc ggaagggttt tgtcgttgat gggccatgag
    13561 tgttcaagga ttgtagtggc gcagcccccg ttcagtaaag ccaagtcttg cgccaatact
    13621 ggcctcgctt actttcgaac ttttcgcact cctttaatcc cgctatcatt gtaaaggcgc
    13681 ccgccgcggc gggctgcgtc ctttaatagg ttcgggcgaa gcaccgcatg cagtcttttc
    13741 ttccctgcgg tctcggtgca ctccgcgaac tctgtgtaaa ttgtttttat ttcaatgcgg
    13801 tggttctaga tgcctagctt cttcatcgaa caattcggtt gccgcgccac ccaagccgat
    13861 ggcgctgccc tcgagcgcca gttgcttgac cgtgggtgta cctccgcctc cacggccgcc
    13921 gccgacatca tcgtcgtcaa cacctgcacg gtcacagcct ccgccgacgc ccaggcccgc
    13981 gacgccatcc gaaagctcca cgccgcaaac cccgccgtcc gcgtcatcgt cactggctgc
    14041 tatgcccagc gtgctcccga agagttggcc gcactcccgg gcgtctcctg ggtagtcggc
    14101 aattcccaca agccccaaat cccccagcta gtcgattcac taacgactcc cgcgtctcct
    14161 gaatccgcga acaacttttt ccccgccgct gcactgcact ctgactcgac tgtccccttc
    14221 cattccttcg tacgggcgca gcatgctgcg ccccagctcg gcaagatcct gaccggcgac
    14281 atctgcgacc gcaccgaatt tctctccgcc cccgtcctcg gcggagaagg gaatcacacc
    14341 cgccccaccc tcaaaatcca ggacggctgc gactcccgct gctcctactg cgtaatcccc
    14401 ttcgtccgag gcaaaagccg cagcctccca cccaacaccg tcatccaaga aatccagcgc
    14461 ttggccgaag cggaataccg cgaaatcgtc ctcagtggca tcaacctcgg cacctacggc
    14521 cgcgatctct caccccgcgt cgaattcgaa atcctcctcc gccgcatcct cgacgaaacc
    14581 tccgtcgagc gcctccgcat cagctccatc gagcccctcg acgttaccca agacctcgtc
    14641 gctctcttcg cttccaccga tcgcatcgcc cagcacttcc acatgccttt gcagtccgcc
    14701 tctgaccgca tcctcgccgc catgcaccgc tggtaccgcg cggagcacta cgcccgccgc
    14761 gtcgaactca ttcacgagcg cctacccaat gctgccatcg gcgccgacgt catcaccggc
    14821 ttccccggcg aatccgaaga cgaccatgct gcgacgattg cctttatcga acgcctgccc
    14881 ttcacctatc tccacgtttt ctctttttcc aagcgtcccg gcaccaaagc cgcctcgctt
    14941 gcaaacgaag tcaccggctc cacaatcaag cgccgcgccc gcgaactccg cgccctcagc
    15001 gaaaaaaaat ccgccgcctt ccgccattcc caactcggcc gcaccctccg cgtcctcacc
    15061 ctccgcccca gcgacgatcc ccgttcaaac acaacgcccg ccctttcaag taactacctc
    15121 cgcgtccgca tcgtcggagc gctttctgca aacctctggg tagacgttca ggttacaggc
    15181 gaggaggaaa tgttcttgat cggaacaggg atttgtgacg ggcagggaca gcatgcacga
    15241 attccggcga atgtatgaat gctgctccca ttgcggcctt gatccttggc atcatctttt
    15301 ggttcggcat tggtattgtt tgcctgagtt ggccaaagag gatccaaagt cttgcgataa
    15361 ggtcatctgc gcgatggcct tcttggtatc tgagatattg gccattcgct gcgtggtcga
    15421 aggagtggat aggtaggcca agttatattt ggtatctgag aggcgtgggg attttctgcc
    15481 ttgcgattgg ttcctttctg ctcttccttg tagccgtcag ataaagcttc gttctctaca
    15541 gcaagtattc ggcacttcgt ttttggtttt ccccctccaa cctccctggt gaattcttct
    15601 aactttcaac tctcgaaaat cacctgcgcc agcgcctgcg ctgccgtatg cgtgatgcta
    15661 agcgcgacac tgttcactcc gagctgtttc gcgatctttg ctgcttcacc cgtcagtgcc
    15721 agggtcggcc tcccgctcgt ctcccgcatc acttccaaat ccaaccattt cactccgcgt
    15781 ctccagcccg tccccagcgc cttcatcgct gcctcttttg ccgcaaatcg ccccgcatac
    15841 cgttcatact tattcttgaa ccgctcgcag tactctcgtt ctctcggcgt ataaatccgt
    15901 ctcagaaata cttcgccatg gcgctcaatc gccgcctgga tgcggtccac ctcagcaata
    15961 tccacgccca ttccaatgat caagatgcct ccagcgctcc cgcgttcggc ccagcggccc
    16021 actccgatta tactgttaga gttgtggctc ccacgaaaac actaaagcct aaacgcaaag
    16081 tccgcgaacg ccccgcggta ggacagacat gcctgtctgt ctctttcggc cgatccaact
    16141 ctaactgggt cgaacgcaaa gtccgcggcg ttcaaatcct tcaattaccc accctcacca
    16201 aaatcccctg gctcgtccac ggcttcagca cccgcccggg cggagtcagc ctcatggaca
    16261 aagaaaaagt cctaaatctt ggtttcaccg aatgggacac caaggaaaac gtcctcgaaa
    16321 actgcctccg cttccaatca gccctcggcg catccaacct caaactcatc tccctgaaac
    16381 aaatccattc cgatgtcatc catcttttcg acgccgcacc cgcccaacct tgccaaggcg
    16441 atgcctcctc gacgaaccgt cccggcctcc tcctcggcgt gcaaaccgca gactgcgtcc
    16501 ccattctcct cgttgatccc caaaagcgcg ccatcgccgc cgttcacgct ggctggcgcg
    16561 gcactctcca gcgcatcgtc gtcaaagcca tcggccagat gcaaatgcaa ttcaaatcca
    16621 agcccgccga cctcctcgcc gccatcggcc ccgcgatcgg cggctgctgc tacgaagttg
    16681 gcaccgaagt cgctatccag tttcaatccc aattcgccga cgcccacgaa tggttcgacg
    16741 aattccgcac cggcgacgaa cccaatccca tccagtggct caacatgatg ccccccggcc
    16801 accagcctcc gcccaaaaac gtccttctcg acctccgcaa agccaatcgc gcccaactcc
    16861 tcggcgccgg cctccgcgcc cccaacgtct tcgtcagcga tctctgcacc gcctgccgcc
    16921 gcgaccttct tttcagctac cgcaaggaag gcgccctgag cggccgtctc atgtccgtca
    16981 tcggtatcca gccctcctaa accagcccgg atacgaccct ctgatgtatc tctttttcaa
    17041 gaaaacccca tttctcactg tggctaattt tctgttgaca acccaatcac gactctgcta
    17101 gattatccct ataaagtagt gcgcctcgcc ttccgcgaga ctccccttac aactcaaatc
    17161 tttcatttct ggagacttcc atgtccacta accgctccaa ggaccgttcc cgtttgtgtg
    17221 ctttcacctt tgccgatggc cgccgatgcc gcactccgcg ttgcgccggc cacccgcacc
    17281 tctgcgcgtt ccacgcccaa aaggaagcgc aatcccgcgc cgccgcgcag gccggggagg
    17341 acgtatctta cttcctctcc ggcacttatc tgtccgcctg cgatctcact tccgctttgg
    17401 gccgcctttt ctccgccgtc gcccaaggcc aagtcaaacc caaaaccgcc accgccctcg
    17461 cctacctcgg ccagactctc aatcattcta tccagctcgc ccagcacgaa tttatcaacg
    17521 cctacggc
//
LOCUS       DASYVO010000532         5297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_154467, whole genome shotgun sequence.
ACCESSION   DASYVO010000532 DASYVO010000000
VERSION     DASYVO010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154467"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1653)
                     /gene="carB"
                     /locus_tag="VGR03_18350"
     CDS             complement(<1..1653)
                     /gene="carB"
                     /locus_tag="VGR03_18350"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV1996302.1"
                     /translation="MPKRKDIKKVLIIGSGPIIIGQACEFDYSGAQACKALRAEGYEV
                     VLVNSNPATIMTDPELAHRTYIEPLTKEYLEEIISRERPNALLPTVGGQTALNLAVEL
                     SESGILEKYKVEMIGASLRAIKVAEDRLWFKDACRKIGLEVPASALVNNAKDALRLCD
                     QLGFPLVIRPSFTLGGTGGSIAYNKEEFGEAIAHALDMSPVHEALVEESVLGWKEYEL
                     EVMRDFRDNFVVICTIENFDPMGVHTGDSITVAPAQTLTDKEFQRMRDASAAIIREVG
                     VETGGSNVQFAVNPENGKMVVIEMNPRVSRSSALASKATGFPIAKIAARLAVGMSLDE
                     IPNDITKKTPACFEPTIDYVVVKIPKWQFEKFPGADSTLGTQMKSVGEVMAIGRTFKE
                     ALQKGIRSLEPSTPWRAPSETPDSLLREKLATPRPDRIHWLLTAMERGLPAQEICEIT
                     KIDPWFIQQIEELTAMNRRAAAVTVETASKELLREVKRNGTSDEQLAQIWRTTSASVR
                     LQRARYGVTPVFKRVDTCAAEFESFTPYLYSTYEDEDESDAQA"
     gene            complement(1674..2873)
                     /gene="carA"
                     /locus_tag="VGR03_18355"
     CDS             complement(1674..2873)
                     /gene="carA"
                     /locus_tag="VGR03_18355"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012053752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV1996303.1"
                     /translation="MREDPLTEVQHRPAILALEDGRVFNGRAAGAITRRGGEVVFNTS
                     LTGYQEVFTDPSYAGQIVCLTYPHIGNVGANVEDEESTRPYIESLIVREFSQVSSNWR
                     SSETAQLYLNRHKIPVIWDIDTRALVRHIRNVGALRGIVSTDGTPAEQLVFEAKQVPS
                     MAGQELASHVTCGKSYGWDKGSVEVAISPWSEQMGESDAHAETRKHRVVAYDYGIKQN
                     ILRLLVDHHCEVFVVPAQTSAADVLEMKPDGVFLSNGPGDPEPVTYAVENIQKLIGRV
                     PVFGICLGHQLCGLALGGKTFKLKFGHHGSNHPVKNLLTEQVEITAQNHGFCVDPESL
                     PSSKVEITHINLNDNTNEGMRHKSLPLFSVQYHPEASPGPHDSHYLFTQFTDLMKEFA
                     QPGSSRH"
     gene            complement(2894..3589)
                     /gene="lepB"
                     /locus_tag="VGR03_18360"
     CDS             complement(2894..3589)
                     /gene="lepB"
                     /locus_tag="VGR03_18360"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV1996304.1"
                     /translation="MERPDEKHPATSTSPETPAPASCSVVTADQLTATVRIEQVDSNA
                     AAGRSAPLHALRNEIRVWTRDLLIAIGLALVIIVFLYQPVKVEGTSMAPLLSDQERIF
                     INKFVYRFEAIQRGDVVVFWYPLDHSKSFIKRVVGLPGEAVEIRQGAVYVNDKIVLEP
                     YVPPQYEDLSDFGPVRVPKDSYFVMGDHRISSNDSRVFGPVASRFIYGRAVFAYWPVE
                     HFGSLSTTEANTK"
     gene            complement(3784..4503)
                     /locus_tag="VGR03_18365"
     CDS             complement(3784..4503)
                     /locus_tag="VGR03_18365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEV1996305.1"
                     /translation="MRRRFFVERFAGESAVMEGEAAHHLGRVLRAQTGQLYELSDGEH
                     VWLGRIESVGRDRVQFALLEELPAIHPSAEVTLLLAVVKYDAFEWAIEKATELGVSAI
                     VPLAAGRSEKALLTAAAKRAERWKKILLEASQQSRRVRVPVLGDLTRPDSAFASRKEG
                     LRVMLSERATASPLRKVLEGQRATKAILAIGPEGGWTDAEFAAAQSCGFLEASLGRLI
                     LRTETAVITALASLNYALDDE"
     gene            complement(4533..>5297)
                     /gene="dnaJ"
                     /locus_tag="VGR03_18370"
     CDS             complement(4533..>5297)
                     /gene="dnaJ"
                     /locus_tag="VGR03_18370"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV1996306.1"
                     /translation="LRYDMSLTFEEASAGVTTKIKLPRQEFCEACNGTGAKKGTGVQA
                     CQTCAGRGQLAYQQGFFTITRTCPACQGAGQIVRERCQECRGQGRLEREKTIELRIPP
                     GVDTGTRLRVAGEGEPGPNGGPSGDLYVVLEVKEHPFFERRGADLYCTIPLSIAQAAL
                     GTELQVPGLNGEERLKIPEGTQSGAVFRIKGKGLADPRGGGKGDLYYHVRVMTPTKLT
                     RDQRKLIEQLGATMKMENKPADRNSSMFDKVKDIFG"
BASE COUNT          978 a         1625 c         1516 g         1178 t
ORIGIN      
        1 cgcttgcgcg tccgattcgt cttcgtcttc gtacgtggaa tagaggtacg gcgtgaacga
       61 ttcgaattcc gccgcgcaag tatcgacacg cttgaacacc ggcgtcacgc cgtagcgggc
      121 gcgctgcaag cgcacggaag cggacgtcgt tctccagatt tgcgctagtt gctcatcgct
      181 ggttccgttg cgcttcactt cacgcagcaa ttccttcgag gccgtctcga cggtcaccgc
      241 ggccgcgcgc cggttcatgg ccgtcaactc ttcaatctgc tggatgaacc agggatcgat
      301 cttggtgatc tcgcaaattt cctgggccgg cagcccgcgt tccatggccg tcagcagcca
      361 gtgaatgcgg tccggcctcg gagtggccag tttttcacgc agcagtgaat ccggtgtttc
      421 tgacggggcg cgccacggcg tgctcggctc cagcgagcga attcccttct gcagcgcttc
      481 cttgaaggtc ctgccaatgg ccatgacttc gccgaccgat ttcatctgcg ttccgagagt
      541 gctgtccgcg ccgggaaatt tttcgaactg ccacttggga atcttcacca cgacgtaatc
      601 gatggtaggt tcaaagcacg cgggcgtttt ttttgtgatg tcattgggga tctcgtccag
      661 cgacattccc acggccagcc gcgctgcgat ttttgcgatc ggaaagccgg tcgccttgct
      721 ggcgagcgcg gaactgcgcg atacgcgcgg attcatttcg atcaccacca tcttgccatt
      781 ttccgggttc acggcgaact gcacgttcga accaccggtt tccacgccga cttcgcgaat
      841 aatcgctgcg gaagcatcac gcatgcgctg aaattccttg tccgtcagcg tctgcgcggg
      901 tgccacggta atcgagtcgc cggtgtgcac gcccatgggg tcgaagtttt cgatggtgca
      961 gatcaccacg aagttgtcgc ggaaatctcg catcacttcg agttcgtatt ccttccagcc
     1021 gagcacggat tcttccacca gtgcttcgtg caccggactc atgtccagag cgtgggcgat
     1081 ggcttcgcca aattcttcct tgttgtaggc gatcgagccg cccgttccgc ccagcgtgaa
     1141 agacgggcga atcaccagcg ggaatcccag ttgatcgcag agccgcaagg cgtccttcgc
     1201 gttgttcacc agcgcggacg ccgggacttc cagcccgatc ttgcggcagg cgtccttaaa
     1261 ccagagacga tcttccgcga ccttgatggc tcgcagcgac gcgccgatca tctccacttt
     1321 gtatttttcg aggatgccgc tttcggaaag ctcgacggct agattcagcg ccgtctgccc
     1381 accgaccgtt ggcagcagcg cattcggtct ttcccgcgag ataatttcct cgaggtattc
     1441 cttcgtcagt ggctcgatgt aggtgcggtg cgcgagttcc gggtcggtca tgatggtcgc
     1501 cggattcgaa ttgaccagaa cgacctcgta gccctccgcg cgcagggcct tgcacgcctg
     1561 cgcaccggag taatcaaact cgcaggcctg gccgataatg atcggccccg acccgataat
     1621 cagaactttt ttgatgtcct tgcgcttcgg catattctcc cgtgaatcct ttttcagtga
     1681 cgcgaactgc ctggctgcgc gaattccttc atcaaatccg tgaactgcgt gaacaaatag
     1741 tgcgaatcgt gggggccggg cgatgcctcc gggtggtact gcacgctgaa gagcggcaac
     1801 gacttgtggc gcatgccctc gttcgtgttg tcgttcaaat tgatgtgcgt gatttcgact
     1861 ttactcgacg gcagtgattc cggatcgacg caaaacccgt gattctgggc ggtgatttcc
     1921 acttgttcgg tgagcagatt cttgaccgga tgattcgaac cgtggtggcc aaatttcagc
     1981 ttgaacgttt ttccacccag cgccaggccg cacaactggt gccccaggca aatgccaaag
     2041 accggcacgc ggccgatcag cttctgaata ttttccaccg cgtaggtgac cggctccgga
     2101 tcgcccgggc cgttcgaaag gaacacaccg tcgggcttca tctccagaac gtccgcggcg
     2161 gaagtttgcg ccggcacaac gaagacctcg cagtggtggt ccacgagcag gcgcaaaatg
     2221 ttctgcttaa tcccgtaatc gtaggccaca acgcgatgtt tccgcgtttc cgcgtgcgcg
     2281 tccgattcgc ccatttgttc ggaccagggc gagatcgcga cctcgacgga tcccttatcc
     2341 caaccataag atttcccgca ggtgacgtgg ctggcgagtt cttgccccgc catgctcggc
     2401 acttgcttgg cttcgaaaac caattgttca gcaggtgtcc cgtccgtcga aacaatgccg
     2461 cgcagcgcgc caacgtttcg aatgtggcgc accagcgcac gagtatcgat gtcccagatc
     2521 acgggaatct tatgccggtt cagatatagc tgcgccgtct ccgacgatct ccagtttgaa
     2581 gagacctgcg agaattctcg cacgatgagc gattcgatgt agggccgcgt ggattcctca
     2641 tcttcgacgt ttgcgccgac atttccgatg tgcggatagg tcaaacagac gatctgcccg
     2701 gcatagctgg ggtcggtgaa gacttcctga taaccggtga ggctggtatt gaaaacgact
     2761 tcgccgccgc ggcgcgtgat cgcgcccgcc gcgcggccgt taaagacgcg cccgtcttcc
     2821 agcgcgagta tcgcgggtcg gtgctgaact tcggtcagtg ggtcctcccg tatttctcat
     2881 caaaatgtgc cattcacttg gtgttggctt cggtggtcga aagcgatccg aaatgctcca
     2941 cgggccagta ggcaaaaacg gcgcgcccgt aaatgaaccg gctagcgacc ggaccgaaaa
     3001 cgcgcgaatc attcgagctg atgcggtgat cgcccatgac gaagtaactg tccttgggca
     3061 cgcgcacagg gccgaaatcg ctgaggtcct cgtactgcgg cggaacatag ggctcgagca
     3121 caatcttgtc gttgacgtac acggcgccct ggcggatttc cacggcctct cccggcaagc
     3181 cgaccacgcg cttgatgaac gatttcgagt gatcgagcgg ataccagaac acgaccacat
     3241 ctccgcgttg aatcgcctcg aagcggtaga caaatttgtt gatgaaaatg cgttcctggt
     3301 cggagagcag cggcgccatg ctggtgcctt cgacttttac cggctggtac aagaaaacga
     3361 tgatcaccag cgcgaggccg atggcgatca ggaggtcgcg cgtccagacg cgaatttcgt
     3421 tgcgcaaggc gtgcagcggc gccgaccgcc cagcagccgc atttgaatcc acctgctcaa
     3481 ttcttacagt cgccgtcaat tggtcagcgg tcacaacaga acacgatgcc ggagctgggg
     3541 tctccggtga ggtcgaggtc gcgggatgtt tttcgtccgg tctttccatt tttgggtgcc
     3601 ttgtatttat agcatggcca tcgcggcccg cgccagatgc gatttgacgc cgtggcctgc
     3661 ggagggcgaa ggctgccgcg ctctcttacg tccactgaga ttcaccgagc tccgccgttc
     3721 gttgctcgcc ggtatcagtt ttttgtcgaa tggtggaaag gacttgggaa ccaagcccgt
     3781 cgcttattcg tcgtcgagcg cgtagttcag cgacgccagt gcagtgatta cggcggtctc
     3841 cgtgcgcagg atcaatcggc cgagcgaagc ttccaggaaa ccacagctct gagccgccgc
     3901 gaattccgca tcggtccagc caccttccgg accgatcgcc aggatcgcct ttgtcgctcg
     3961 ctggccttcc agaacttttc gtagaggcga agcggttgcc cgctcggaaa gcatcacgcg
     4021 caatccttct ttgcgtgaag caaaggcgct gtctggcctt gtcaagtcgc ccagcacggg
     4081 cacgcgaacg cgccgggatt gctgcgatgc ttccaacagg atttttttcc agcgctccgc
     4141 tcgcttcgct gccgcggtga gcagggcctt ctcacttcgc cccgccgcga gcggcacaat
     4201 cgcactcact cccagctctg tggctttttc aatcgcccat tcaaacgcgt catacttcac
     4261 gacggccagc agcagcgtga cttccgcgct ggggtgaatg gcgggcaact cctcgaggag
     4321 agcgaattgc acgcgatcgc ggcccacact ttcaatccgg ccgagccaaa cgtgttcgcc
     4381 gtcgctcagc tcgtagagct ggccggtttg ggcgcgcaac acgcgtccga ggtgatgcgc
     4441 cgcttcgcct tccatgacgg cgctctcacc cgcaaatcgt tccacgaaaa atcgtcttcg
     4501 cacgcggaga ttctatctca ggaagggtgt tactacccga agatgtcttt cactttgtcg
     4561 aacatcgagg aattgcggtc cgcaggcttg ttctccattt tcattgtggc gccaagttgc
     4621 tcgatcaatt tccgctgatc gcgcgtgagc ttcgttggcg tcatcacacg cacgtggtag
     4681 tacaaatcgc cttttccgcc gccgcgcgga tcggccagtc ctttgccctt gatgcgaaac
     4741 acggcgccgc tctgcgtgcc ttcgggaatc ttcagtcttt cttccccgtt caatcccgga
     4801 acttgcaatt ccgtaccgag agccgcctgc gcaatgctca aggggatcgt gcaatacaag
     4861 tccgcgccgc gccgctcgaa gaacggatgc tctttgactt caagcaccac gtacaaatcg
     4921 cccgacggcc cgccattcgg cccgggctcg ccttcgccgg cgacacgcag ccgtgttccg
     4981 gtatccacgc ccggtggaat ccggagttcg attgtctttt cgcgctcgag gcgtccttgc
     5041 ccgcggcact cctggcaacg ctcgcgcaca atctgaccag cgccctggca cgctgggcat
     5101 gtgcgcgtga tggtgaagaa tccttgttga taggccaact gaccgcggcc ggcgcacgtt
     5161 tggcaggcct gaacgccagt gccctttttc gcgccggtgc cattgcacgc ctcgcagaat
     5221 tcctgccgcg gcagtttgat cttggtggtt acgcccgcgg acgcttcttc gaacgtgagc
     5281 gacatgtcgt atcgcaa
//
LOCUS       DASYVO010000533         7275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_154703, whole genome shotgun sequence.
ACCESSION   DASYVO010000533 DASYVO010000000
VERSION     DASYVO010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154703"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VGR03_18375"
     CDS             complement(<1..948)
                     /locus_tag="VGR03_18375"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HEV1996307.1"
                     /translation="MYFIYSLLMGLAALLLVPYWLVQGLRQRKYLSNLGERLGFSFPA
                     LAKLPAQSTGAIWIHAVSVGEALSSITLARRLKEAYPHRPLIISTTTITGQTLARERM
                     PFADAVIYFPLDWAFCVRRALDAVRPSVVLVLETEIWPNFLTEAGRREIPVLFVSGRI
                     SDRSFARYQSYLGVFGFFLRPFLRNALSNASAFLMQSEKDADRVRALGAPADRVRVSG
                     NLKYDLELPAPTPLSNWLASEIKRSGRSPVIVAGSVVATEEPHALIAFGTLQGEYPKA
                     LLVLAPRKPECFDDAAEFIDESHRKFIRRSRLPIPGPAQA"
     gene            1044..1715
                     /locus_tag="VGR03_18380"
     CDS             1044..1715
                     /locus_tag="VGR03_18380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996308.1"
                     /translation="MRKVYYNPMGKLTLILPFLAASLLAAPQGTSQQPSSLKTASLES
                     HEGMTITARPWTDPALYKEKFPKKSPFAAGIVAIQVVFRNDSDDSMKINLERIRLNLA
                     LSEEDHQALHPLSPEEAADAIVNPGAKDPTKTRKKFPFPTGAPKVGHDKHWAEVEKSM
                     EDASLQSSVVAPHSTVQGLLYFDLRSQFELLNTAHLYVPEIVAIEKNRGLLYFEIDLG
                     RAAER"
     gene            1812..4076
                     /locus_tag="VGR03_18385"
     CDS             1812..4076
                     /locus_tag="VGR03_18385"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEV1996309.1"
                     /translation="MLALLDELNPEQRLAVETTDGPVLILAGAGTGKTRAITFRMANL
                     IATGVPAEAILAVTFTNKAAEEMRNRVSNLLMRAGIPPAQPWLSTFHSLCARLLRREA
                     AAAGLPQDFAIYDDDDQLAAVKLAMNKLQIDAEDLTPRIVLSRISYAKNHGQTAEQLR
                     AEAFDQNGRRTADIFTAYEKLLHDSKALDFDDLLLRSARLLRESTATREKWKARFQYI
                     HVDEYQDTNRVQYELMRLLTGPKQNVCVVGDEDQSIYRWRGADVSILLSFSRDFPAAR
                     IVKLERNYRSTQNILDAAGAVVANNPERLGKSLSAEKGPGSNLKYLEARDAQAEAEFV
                     AGELERILNDDSDQTCAVEYRTNFQSRAFEEVFRRRGLRYKLVGGFSFYNRAEVKDAL
                     AYVRLAMHPEDDISLLRVLNVPPRGIGKTTVDALREGARVDGIPLWAAIERFVSGASA
                     GRAITPLRAFQDLIRKLQDALAAKEPSGFLHAVLEESGYMAMLKDRNTPEDVARLENL
                     EELARAVAEGMEAGETFTDFLDAAALVSDADQFEGKPGVTLITLHSTKGLEFDHVFLT
                     GMEEGICPHSRAITEEKGIEEERRLVYVGMTRARQSLTLTRAVYRRIFGNEQQLRASL
                     PSRFLAEIPSELVDTVRGSMAEIGETRRYEPDPEYSYSPEEFLRRVRGGPKQAEAAPR
                     RQPAAQNFFGRPAAKRGGDANPMLGRKVRHPSFGVGTIVGVEGDDEDRRVSVSFPGRG
                     TKKFIERYAQLEQA"
     gene            4235..5770
                     /locus_tag="VGR03_18390"
     CDS             4235..5770
                     /locus_tag="VGR03_18390"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV1996310.1"
                     /translation="MASQLFRCKSIDQLLSDSENAEVRLKKSLGWLSLTSLGIGAVIG
                     SGIFTIIGTAIAGQKFQASSILNAPLLEYLIHHSASFGRPGAGPAIALSFILVAIACG
                     FASVCYAELAAMIPIAGSAYTYTYATMGELIAWIIGWDLILEYAVSNMAVSVGFSQHM
                     VNLLDWFGVHPNPCWISPAYLPSGLSDLQGNMLYAAGWHFGFNWPAFIIVMLLTVILV
                     RGIKESARTNNIMVLLKIIAILVFVGFASKLIHPANYHPFAPNGWPGILTGGSIVFFT
                     YIGFDSVSTAAEECKNPQRDLPIGIIATLVVCTLLYIAVVVVLTGLVKWDTLVDDAAP
                     VVNTLKKLHFTGVRLVVLIGALMGMISSLLVFQLGQARVWFAMSRDGLFPRIFGRVHP
                     RFQTPDFSTWMAGFVVGIPAGLLDIGTLADLSNIGTLFAFALVAGGVLILRYREPDRP
                     RAFRAPGGPLAPVLTIVTCLLLMAGLPIMNWIRFFVWMILGLVLYYFYGRKHSTLRAA
                     NRA"
     gene            5784..6641
                     /locus_tag="VGR03_18395"
     CDS             5784..6641
                     /locus_tag="VGR03_18395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996311.1"
                     /translation="MQGLSFQQAGRRAVVALASFVLFLSSPQSSQAWGKNGHRLVVNK
                     AIDTLPQDIRSFFESNLALLSQHVTDPLDAIAKTPAERHNHFILLDKYGRFPFEALPR
                     NYKAALTKFGKPKLDANGLLPWQIGVYSEKLTEAMKAGKWDEARLDAAILANYVAEAH
                     DPFNTTDNFDGRLSGQPGVNDRFGTTLIDRYSSFFPMRPNDAIFINDPTDRAFEACLS
                     SHSWLETILLADRHARHGENSFTDEYYDRFYNQAAAILIRQLSDAATDVGSFWLTAWV
                     NAGRPQLPH"
     gene            6652..>7275
                     /locus_tag="VGR03_18400"
     CDS             6652..>7275
                     /locus_tag="VGR03_18400"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996312.1"
                     /translation="MTPPVLSNRIASLPKAELHLHLEGSIQPATVCALTARHGVVMTE
                     EEVRRRYAYRDFPEFIEAFKWVTSFLREPQDYALIARDLAEHLLTQRVVYTEVTLSVG
                     VMLLRKQRPEANFEALLRATEPFENRGLRFQWIFDAARQFGADAAMEVVESAKRCNSK
                     SIVAFGIGGDELSVPTKEFRPVYDRAAQMGLHRLMHAGEVGGPEKIRE"
BASE COUNT         1531 a         2232 c         2057 g         1455 t
ORIGIN      
        1 cgcttgcgcc ggcccgggaa tcggcaatcg cgaccgccgg ataaacttgc gatgcgattc
       61 atcgatgaac tccgccgcgt catcaaagca ctccggcttg cggggcgcaa gcacgagcag
      121 cgccttcgga tactcgcctt gcaaggttcc aaacgcgatg agcgcgtgcg gctcctccgt
      181 cgcgacgacg ctaccggcga cgatgaccgg cgatcggccg ctgcgtttaa tctcgcttgc
      241 gagccaattc gacaatggcg tgggcgcggg aagctccaag tcgtatttca gatttccgct
      301 gacccgcacg cgatccgccg gcgcgcccag ggcccgtacg cgatccgcat ctttctcgct
      361 ctgcataagg aaggcgctgg cattggacag tgcgtttctc aggaaaggac gaaggaaaaa
      421 gccaaaaact ccgagataac tctggtagcg tgcaaacgag cggtccgaaa tgcgtccgct
      481 gacgaaaagt acgggaatct cacgccgccc ggcttccgtc agaaaattcg gccagatctc
      541 cgtttcgagc accagcacga ccgacggccg cacggcatcg agcgcgcgcc ggacgcagaa
      601 ggcccaatcg agcggaaaat agatgaccgc atcggcaaac ggcatgcgtt cgcgcgccag
      661 cgtctgcccc gtaatggtgg tcgtcgaaat aatgagaggc cgatggggat aggcttcttt
      721 caaccgccgc gcaagagtaa tgctagaaag cgcttcgccg acactcacgg catgaatcca
      781 gatcgcgccc gtcgattgcg cgggcagctt ggcgagcgcc ggaaaggaga atcccaggcg
      841 ttccccaagg ttagagagat actttcgttg gcgcagtccc tgcacgagcc aatagggcac
      901 cagcaaaagg gccgcaaggc ccatgagaag gctataaatg aaatacacta agcgtggctc
      961 cggtctgtac gtgctccgcc ataagcaggc ggcgcgcgaa gcagggcaac cctgcacgac
     1021 ccccaggaat cgaaggcctc gagatgagaa aagtctatta taacccgatg ggaaaactaa
     1081 ctctcatcct tccctttctt gccgcttccc tgctggccgc cccacaggga acctcccagc
     1141 aaccgtcctc gctcaagacc gcatctctgg aatcccacga gggtatgacc attacggcgc
     1201 ggccgtggac cgaccctgca ctctataagg agaagttccc gaagaagtcg ccgtttgcag
     1261 caggaatcgt cgccatacag gtcgtttttc gaaatgattc cgatgacagc atgaagatca
     1321 acctggagcg cattcgattg aacctcgcac tctccgaaga agatcatcaa gcgttgcacc
     1381 cgctttcgcc ggaagaagcg gccgacgcga tcgtaaaccc gggcgctaag gatccgacca
     1441 agactcgaaa aaagtttccc ttccccaccg gagctccaaa ggtcgggcat gacaaacatt
     1501 gggccgaagt ggagaagtcc atggaggacg caagcctgca gtcgagtgtc gtcgcgccgc
     1561 acagcaccgt gcaaggactt ctttatttcg acttgcgcag ccaattcgag cttctgaata
     1621 ccgctcatct ttacgtaccc gaaattgtgg ccatcgaaaa gaatcgcggc ctcctgtact
     1681 ttgaaattga cctgggtcgg gccgccgaac gctgagccat ccgaaaccga aggcgaattg
     1741 cacaagtttg cgcgcatcgc cgccgtcggc taacatgacg tgtgcaaact ttctccaacc
     1801 accgcggaca aatgctggct cttctcgacg aactgaaccc cgaacagcgg ctggccgtcg
     1861 aaaccaccga tggcccagtg ctaatcctcg cgggtgctgg aaccggcaag acgcgcgcaa
     1921 ttacttttcg catggccaac ctgatcgcca ctggcgtgcc tgccgaagct atcctcgccg
     1981 tcacgttcac caacaaagcc gcggaagaaa tgcgcaatcg cgtctcgaac cttctgatgc
     2041 gcgcggggat acctcccgcg cagccctggc tttccacgtt tcattcgctt tgcgcgagac
     2101 tgctgcggcg cgaggcggcc gcggccggtc tgccgcaaga ttttgccatt tacgacgacg
     2161 acgaccagct cgcggccgtc aagctggcga tgaacaagct gcagatcgat gcagaagatc
     2221 taacgccgcg aatcgttttg agccggatca gctacgcaaa aaatcacggc cagaccgcgg
     2281 agcaattgcg cgccgaagca ttcgatcaga acggacgccg caccgccgat attttcacgg
     2341 cctacgagaa actcttgcac gacagcaaag ctctcgactt tgatgacctg ctcctccgct
     2401 cggcgcgtct gctgcgcgaa tccacggcca cgcgggagaa atggaaagcc cgcttccaat
     2461 acattcacgt ggacgaatat caggacacga atcgcgtgca atatgaactg atgcggctgc
     2521 tgaccgggcc gaagcagaac gtttgcgttg tgggcgacga agatcaatcg atctaccgct
     2581 ggcgtggcgc cgacgtttcc atcctcctga gtttttcccg ggattttccc gccgcccgga
     2641 tcgtcaagct ggaacgcaac taccgttcga cgcagaatat tctcgatgcg gccggcgcgg
     2701 tcgtggccaa caatccggag cggctgggca agtcgctgag cgcggagaag ggcccggggt
     2761 cgaatctgaa gtatctcgag gcgcgggatg cgcaggccga agcggaattt gtcgccggcg
     2821 aactcgagcg cattctcaac gacgattcgg atcagacgtg cgcggtcgag taccgcacga
     2881 atttccagtc gcgcgcattc gaagaggttt ttcgccggcg tggcctgcgc tacaaactcg
     2941 tgggtggatt cagcttctac aaccgcgccg aagtgaagga tgcgctcgcc tacgtgcgcc
     3001 tggcaatgca tcccgaggat gatatttcgc tgctccgcgt attgaatgtg ccaccgcgcg
     3061 gcatcggcaa gacgacggtg gacgcgctcc gcgaaggcgc gcgtgttgac ggaattccgc
     3121 tgtgggcggc cattgagaga tttgtctccg gcgcatcggc aggccgggcg ataactcctt
     3181 tgcgggcgtt ccaggacctc atccgcaaat tgcaggatgc gcttgccgct aaggaaccct
     3241 cgggattcct gcatgccgtt cttgaggaat ccggctacat ggccatgctc aaggaccgca
     3301 acacaccaga agatgtcgca aggttggaaa acctcgaaga gcttgcgcgt gcggtcgccg
     3361 aaggaatgga agcgggagag acgttcaccg atttcctcga tgccgcagcg ctcgtgagtg
     3421 acgcggatca atttgaaggc aaacccggcg taacactcat caccttgcac agcaccaagg
     3481 gcttggaatt cgatcacgtg tttctcacgg gcatggaaga aggcatctgc cctcacagcc
     3541 gggccatcac cgaagaaaaa ggaatcgaag aggaacggcg tctcgtttac gtcggcatga
     3601 cccgcgcgcg gcaatcactc acgctgacgc gcgccgtata ccggcgtatc ttcggcaatg
     3661 aacagcaatt gcgagcttcc ctgccgtcac gctttcttgc ggaaattcca agcgagctgg
     3721 tggatacggt tcgcgggtca atggcggaaa tcggcgagac gcgccgctac gagccagacc
     3781 cggagtattc gtattcgccg gaagaattcc tgcgtcgtgt gcgcggcgga ccgaagcagg
     3841 cagaagcggc tccgcgccgc cagccggcgg ctcaaaactt cttcggacgc cccgctgcaa
     3901 aacgcggcgg cgatgccaat cccatgcttg gacgcaaagt tcgtcatccg agcttcggcg
     3961 tcggaaccat cgtgggcgtc gagggcgacg acgaagaccg ccgcgtatcc gtgagcttcc
     4021 ccggtcgcgg cacgaaaaag tttatcgagc ggtacgcgca actcgaacag gcatgatgtg
     4081 gagaatctgc ccctgaacgc gagtgcgcca gcagtttgcg ccgaccggtt gcgtgatgga
     4141 gcttctcggg gtacattgag gcgcgttcat tcctcaaaag cgtgctgggt aatttcagtc
     4201 ttccggtttg cccggatgcg atcggagagc aggggtggca tcacaactct tccgctgcaa
     4261 gagtatcgat caactgctgt cggattcgga aaatgctgaa gtccgattaa aaaagtccct
     4321 gggatggctg agcctcacgt cgctcggcat cggcgccgta atcggctccg gcatctttac
     4381 catcatcggc acggccatcg ccggacaaaa gtttcaagcc tcgtccatcc tgaacgctcc
     4441 tcttcttgag tacctgattc accactccgc ttcgtttgga cgcccgggcg cggggccagc
     4501 cattgctctc tcctttatcc tggtcgcgat cgcctgtgga ttcgcatcgg tgtgctacgc
     4561 cgagctcgcc gccatgattc ctattgcggg cagcgcgtac acctacacct acgccaccat
     4621 gggagaactc atcgcctgga ttatcggctg ggatctcatc ctggaatatg ccgtcagcaa
     4681 catggccgtc agcgtcgggt tttcacagca catggtcaac ttgttggact ggttcggcgt
     4741 gcatccgaac ccctgctgga tttcccccgc ctaccttccg agcggacttt ccgacctgca
     4801 ggggaacatg ctctatgctg cgggctggca ctttggtttt aactggcccg ctttcatcat
     4861 cgtgatgctt ctaacggtga ttctggtgcg cggaatcaag gagtcggcgc gcaccaacaa
     4921 catcatggtg ctgctgaaaa tcatcgccat tctggtgttt gtggggtttg catccaaact
     4981 catccacccc gcgaactatc atcccttcgc gccgaacggc tggcccggaa ttctgaccgg
     5041 cgggtcaatc gtgttcttca cctacatcgg attcgattcg gtctccacgg ccgccgagga
     5101 atgtaaaaat ccgcagcgcg acttgccgat aggcattatc gcgacgctcg tcgtctgcac
     5161 gctgctctat atagccgtgg ttgtggtgct cacgggactg gtcaagtggg acactttggt
     5221 ggacgacgct gccccggtcg tcaacacact caagaaactc cacttcaccg gagttcgcct
     5281 ggtcgtgcta atcggcgcat tgatgggcat gatctcttcc ctgctggtct tccagctcgg
     5341 acaggcccgc gtgtggtttg ccatgtcgcg tgacggcctg ttccccagaa tttttgggcg
     5401 cgtgcatccg cgctttcaga caccagattt ttcaacgtgg atggcgggat tcgtggtcgg
     5461 tattccagca ggcttgcttg acatcggcac gctggccgat ctctcgaata tcggcactct
     5521 gttcgcgttc gccctggtcg cgggaggcgt gcttattctg cgctatcgtg aaccggaccg
     5581 accgcgagca tttcgagccc ccggcgggcc gctggcaccc gtccttacga ttgtcacatg
     5641 cctcttgcta atggccggcc tgccgatcat gaattggatc cgcttctttg tctggatgat
     5701 cctgggcctg gtgctctatt atttttacgg ccggaagcac agcacactga gggcagcgaa
     5761 tcgcgcgtaa gaggaatcgc agaatgcagg gtttgtcttt tcagcaagcg gggaggcgcg
     5821 cagtcgtcgc gctcgcttcc tttgtgctct tcctttcctc tcctcaaagt tcgcaagcgt
     5881 ggggaaagaa cgggcacagg ctggtcgtta acaaagccat cgacaccctg ccccaggaca
     5941 tccgctcgtt tttcgagtcc aaccttgctt tgctctcgca gcatgtaacc gatccgctgg
     6001 atgccatcgc caagactccc gctgagcgcc acaaccattt cattcttctc gacaaatacg
     6061 gacgattccc gttcgaggct ctgccgcgca actataaagc tgcgcttacg aaattcggta
     6121 aaccaaaact ggatgccaac ggccttcttc cctggcagat cggcgtttac agcgagaagc
     6181 tgaccgaagc gatgaaggcc ggcaagtggg acgaagcaag actggacgcc gccatcctgg
     6241 caaactatgt cgcggaagcc cacgaccctt tcaacaccac ggataatttc gacgggcgcc
     6301 tgagcggcca gccgggagtc aacgaccgct tcgggaccac gctaatcgac cgctattcgt
     6361 cgtttttccc catgcgcccg aacgacgcga ttttcatcaa cgacccgacg gatcgcgcct
     6421 tcgaagcatg cctcagctct catagctggc tggagaccat acttctcgcc gaccgccacg
     6481 cgcgtcacgg agagaattcc ttcaccgacg agtattacga ccgcttctac aatcaagccg
     6541 cggcaatttt gattcggcaa ctctccgacg cggcaaccga tgtgggatct ttctggctta
     6601 cggcgtgggt aaacgccggc cggccacaat tgccgcattg atctttcgcg gatgacgccg
     6661 cccgtgctct ccaaccgcat tgcttccctc cccaaggccg agttgcacct tcatctcgaa
     6721 ggctccatcc agcccgcaac cgtctgcgcg ctgacggccc gtcacggcgt tgtcatgacc
     6781 gaggaagaag tacgccggcg ctatgcctac cgcgacttcc ccgaattcat cgaagccttc
     6841 aaatgggtca cttcattctt gcgcgagcct caggactatg cgctcatcgc ccgcgacctt
     6901 gccgaacatt tgctcacgca acgcgtggtc tatacggaag taactctctc cgttggcgtg
     6961 atgctcctca gaaagcagcg gccggaagca aatttcgaag ccctcctccg cgccaccgag
     7021 ccattcgaaa accgtgggct ccgattccag tggatatttg acgcggcgcg ccaattcggc
     7081 gcggacgcag cgatggaagt cgtcgaatcc gcgaagcgct gcaattcgaa atcgatcgtg
     7141 gccttcggca ttggcggcga cgagctcagt gtcccgacaa aggaatttcg tcccgtctac
     7201 gaccgcgccg cgcaaatggg tctacaccga ttgatgcacg caggcgaggt cggtgggccg
     7261 gagaaaattc gcgaa
//
LOCUS       DASYVO010000534        18638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_11359, whole genome shotgun sequence.
ACCESSION   DASYVO010000534 DASYVO010000000
VERSION     DASYVO010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 18638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18638
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11359"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VGR03_18405"
     CDS             complement(<1..477)
                     /locus_tag="VGR03_18405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996313.1"
                     /translation="MKLRGVLFRFTAMRKIAREAVIFALLGAIFASIWFFIQENGTFR
                     RAEAERAQSEVSAAAASDDPAYRILFNAQNAENETRTEAWRIFHESENMEVLNRRLDL
                     LNLPQVVKPDLRDAKARCYPDFIPFPSNPRKTESVLPKGFIPIYCTKFQLPAGLTPL"
     gene            complement(1056..1355)
                     /locus_tag="VGR03_18410"
     CDS             complement(1056..1355)
                     /locus_tag="VGR03_18410"
                     /inference="COORDINATES: protein motif:HMM:NF044550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="HEV1996314.1"
                     /translation="MKRKTSASHDEAIVRRLRKDPNFAAEYLKVALKDEDEPLVLLIA
                     LRHVAQARGIAKVAKAAGVERESVYRALSIRGSPRLSTLVAVAKAIGLRLTVESA"
     gene            1584..1808
                     /locus_tag="VGR03_18415"
     CDS             1584..1808
                     /locus_tag="VGR03_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996315.1"
                     /translation="MEVHFTPEQEERLSKIATKEGVDPQKLVKDAALRLLEDDSRFRA
                     GVRKGIEQADRGQFVEEEEMDARVKQMFQP"
     gene            1808..2083
                     /locus_tag="VGR03_18420"
     CDS             1808..2083
                     /locus_tag="VGR03_18420"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1996316.1"
                     /translation="MRFSWTLAAASDLEHIKDYLTEHHPQFAQPTVLELYETIRSLKT
                     SPHRGRVGREEGTRELVFTRLPYIVAYRIKDQTVEVLHIFHAAQFRP"
     gene            complement(2110..3156)
                     /gene="aroF"
                     /locus_tag="VGR03_18425"
     CDS             complement(2110..3156)
                     /gene="aroF"
                     /locus_tag="VGR03_18425"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1996317.1"
                     /translation="MIISMKLHATREQIDEVRQQVAHFGYKVHSIEGEERVVIGVVGV
                     GDVTACFESVEAMPQVEKVVRISAPYKFVSKEFRAGKTKIKVNGTEIGGSEFVVMAGP
                     CSVESEKQIMQAAEGVAKAGAKMLRGGAFKPRTSPYDFQGMEEEGLKLLKKAKEATGL
                     AIITEVMSDVHVDLVANYADVMQVGARNMQNFMLLKALGKCGRPVLLKRGLSSTVKEL
                     LMSAEYITAHGNSDVILCERGIRTFETVTRNTCDIAAIPALNELTHLPVILDPSHATG
                     RRSLVPALCRAGVAIGADGLIVEVHPAPEKAISDGAQSLDLPQFQKMMKDLEPYIQLW
                     NKSRKEETAAAASH"
     gene            complement(3443..4195)
                     /locus_tag="VGR03_18430"
     CDS             complement(3443..4195)
                     /locus_tag="VGR03_18430"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEV1996318.1"
                     /translation="MRNSKFLLAAAFLFFFSASANAQLQRNDSERQLFEALNRERTAQ
                     GLSALHWDDALFKAARLHALRMADLNMLEHQLPSESNLEGRLAEAGARFSVIAENIAI
                     GPNPQIIHAGWMNSPGHRKNILDPRLTSVGIAAVHGPGGLFAVQDFSQLVPELSVEEQ
                     EQKVIFLLNAEGFRSANATDGARKTCETDAGFAGTSARAMMRFEVADLNKLPEDLEKK
                     IRSGPYSKAAVGACRANAAAGFSRYRIAILFF"
     gene            complement(4207..4929)
                     /locus_tag="VGR03_18435"
     CDS             complement(4207..4929)
                     /locus_tag="VGR03_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEV1996319.1"
                     /translation="MKCNTVKTKLAGYLDDAVTGEARVAERVQIRQHLEGCNVCREEL
                     ERFRKLSVMFSRVPKNIPPADLAVRIKVAAAQAQHLRDWPSRMRRIKDRAEILLDNVF
                     RPLTVPATGGFFSAILVFVLVLQMIGPGTTVRAVQNDVTLNLMRPAELVSLSDYPQAW
                     APEQDDLELSLPHGLLLDVTVDAQGHMTDYQILSGPNSLELRRQLDQMLLFSRFSPML
                     SFGRPTAGGHVVLSFSAVRVRG"
     gene            complement(4926..5687)
                     /locus_tag="VGR03_18440"
     CDS             complement(4926..5687)
                     /locus_tag="VGR03_18440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1996320.1"
                     /translation="MSDWASALSWGRDEADLVHELQAGSETAFDWLVTHYHAPVYNLI
                     LGMLGDTSDAADGSQEVFLKAFRGIRNFRQGSSLKTWLYRIAIREALNHKRWFKRHLQ
                     KNVSIDAEPEEGQARIEVEDLCATPFEQLAALEIQVAVHGALQQIPDVFRSAVILRDL
                     EGLSYEEVSEVLECSVGTVKSRILRGRRALKEILEPLLGGHQLHGIHSQTSSHGNVSE
                     SQPSPFGDAFSAARRVTVSSQGTSPRASSTGKGVQ"
     gene            complement(6474..6743)
                     /locus_tag="VGR03_18445"
     CDS             complement(6474..6743)
                     /locus_tag="VGR03_18445"
                     /inference="COORDINATES: protein motif:HMM:NF018369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HEV1996321.1"
                     /translation="MKLLAAIGVLLFVSVPAHAQAAKSCEELKDEISKKLDAKGVKGY
                     TLEIVAKDKDAEGKVVGTCDGGTKKIIYTKTSAPASTPAKETPKP"
     gene            6945..7406
                     /locus_tag="VGR03_18450"
     CDS             6945..7406
                     /locus_tag="VGR03_18450"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1996322.1"
                     /translation="MRLVLAVVAFSAILAAQKLGSKGTDMEMAHNVSIPELTEAISAR
                     LVAQWEGWKNQDPAPNNAIIADDFNSFWPDGSRHIGKPTAQQMSEQPITGYKLSQFRV
                     VPVGADGALVTYFADIKTPGDNVEHHMAVGEVWVKRNGQWLIRGYSGTLIK"
     gene            7936..8166
                     /locus_tag="VGR03_18455"
     CDS             7936..8166
                     /locus_tag="VGR03_18455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996323.1"
                     /translation="MLNRTKRTICWAALVLCFGATELRGQTAAEADDIPIEPCDVLPV
                     VKVRIDGAEMRFLLDTGATTMLNLKSFTAGRR"
     gene            8163..8792
                     /locus_tag="VGR03_18460"
     CDS             8163..8792
                     /locus_tag="VGR03_18460"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1996324.1"
                     /translation="MTSAREVSLPELALGNHRLQNLRLPAIDLTPIGKACGKQIDGIL
                     GVDLMDKMGVTIDLKRQVASLGAESLDAKCVFELMETAMHHCSTAFETGNAVELAECF
                     DPEIVFYTTQGEYRGRKQVMEYLSQRYLKFAPNLHYRMTLRDVKTFGDALWYSYDYAI
                     DSPQDHIAGHGMSMCRRDGGRWRILNLHNSWVEGAAATKSAGQENIQKQ"
     gene            complement(8865..9389)
                     /locus_tag="VGR03_18465"
     CDS             complement(8865..9389)
                     /locus_tag="VGR03_18465"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV1996325.1"
                     /translation="MVPRTILLRGATLSVLILASLCVLAAPKDKEPAPRFNATTTTGE
                     KFTNESIKGKVVLLEFWTTWCGFCADEAAFVDKIGHELAGKGLILLAIDVSESKKTVK
                     KYLEQHPRNCKIVMMEDTNLAAMYAATAYPIYVVIDRDGFIVGTQRGAGGEEALRNLI
                     ARAGLDSESDSDSQ"
     gene            complement(9665..10078)
                     /locus_tag="VGR03_18470"
     CDS             complement(9665..10078)
                     /locus_tag="VGR03_18470"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEV1996326.1"
                     /translation="MRSMILVDAGPLVALVDSDDQHHAKCVAALKTLREPLATVWPPL
                     TEAMYLLADLPKAQEALWEMIQRGALHLLPLDSADAPRIRELMRKYANRPMDLADAAL
                     VRVAEREGFRKIFTVDRRDFSVYRLHGRIRPTLIP"
     gene            complement(10066..10338)
                     /locus_tag="VGR03_18475"
     CDS             complement(10066..10338)
                     /locus_tag="VGR03_18475"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEV1996327.1"
                     /translation="MASPLTLRLDEKTRQRIARIARRKRLSTSEVIRRAIEAWAEHQE
                     PVGSPFEVMADLIGVVRGGNPKRSEQTGRSLTKLLKGRRDPRRCAR"
     gene            complement(10490..11305)
                     /locus_tag="VGR03_18480"
     CDS             complement(10490..11305)
                     /locus_tag="VGR03_18480"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1996328.1"
                     /translation="MSANPIPEKVDMYNTSYSHLEHPAYREIRLETYGADFGQSNWAS
                     PEEFTEIAGLLQLSKNSSALEVGCGSGGCALHFAESTGCRVTALDVNPEGIKSATALA
                     QSKSLQSRVTFLLHNAATNLPFADATFDAVYSNDAMCHIPDRLAVLREFLRILKPGGR
                     FLFSDALVITGVISDEELATRSSIGHYLFVPRGENERLLEQSGFHLLRAVDTTPNAAS
                     IAKRRHDGRARRQSILIPIEGQVNFDGLQRFLSCVHTLSREQRLSRFLYLTSK"
     gene            11471..12295
                     /locus_tag="VGR03_18485"
                     /pseudo
     CDS             11471..12295
                     /locus_tag="VGR03_18485"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            12403..12816
                     /locus_tag="VGR03_18490"
     CDS             12403..12816
                     /locus_tag="VGR03_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996329.1"
                     /translation="MSGNTERSRTPEWPWPDSLDALTAAPGYHRLLFENERVRVLEVR
                     ISPGQFVPVHTHRWPSMVYVASSSDFIRRDGEGKPILDSRTMGPPPATPTAQWTPPLP
                     PYSVENIGNAEILLITTELKEQALEQRETDCAAKR"
     gene            12800..13651
                     /locus_tag="VGR03_18495"
     CDS             12800..13651
                     /locus_tag="VGR03_18495"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV1996330.1"
                     /translation="MLQNVELIASYWTLAGGGEPHTDREYSTFDFKDRVEAAAKAGFK
                     GVGIWHSDLDHILKRRSLKEMKRILDDNGMKYVELEFLRDWFVDGELKKQSDMERKKL
                     LEASQALNAKHVKVGDFERKKTPMPRLIEAFAELCVEAAKYRAKIGFELMPFGMLDTL
                     KDSLTMIEGAGAKNGGIIFDLWHVAKLGIPYEEVSRVPLKHIVGVEINDGTFTAPWSL
                     HEDTINHRRFCGEGEFDVKGFVAAMEKTGYAGPWGIEVLSAELRPRPLEEAVTKAFKT
                     TIAQFRG"
     gene            complement(13968..16109)
                     /locus_tag="VGR03_18500"
     CDS             complement(13968..16109)
                     /locus_tag="VGR03_18500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEV1996331.1"
                     /translation="MPRKSFPSLAVTLLLLIAAGCRSKSNSPSSAQETVSPANVDASR
                     LLNADQQPGNWMSYGRTYSEQRFSPLKQINDQNVGQLALAWYVDIDTRRGQEATPIVV
                     DGVMYFTTAWSKVFAVNAATGEKLWSYDPKVPPEWAINACCDVVNRGVAVWQGKVFVG
                     TLDGRLIALNAASGKPIWETLTIDPQYRYTITGAPRVVKGKVLIGNGGAEFGVRGYIS
                     AYDANTGKMVWRFYTVPGDPSQPYESPILEKAAKTWTGQWRKFGGGGTVWDSIVYDPD
                     LDLIYFGVGNGTPWDRRARSPQGGDNLFTCSVVALKAETGEYVWHYQETPGDAWDFDS
                     AESIVLADLNINQSPRKVLLHAPKNGFFYVLDRGTGELISAKPYTAVSWASGIDLKTG
                     RPVENTQARYGEGKGIPVAPGPLGGHSWHSMSFSRITRLAYIPVQDSGFLYKSDQHSQ
                     PSDLAFNTGIDFVAAGMPQKPEVKKAILGAIKGHLSAWDPVQQKEIWRVDRQSPVNGG
                     ILSTAGNLVFQGTAQGNLEAYRADNGQKLWSADAQSGVVAAPITYSVNGEQYVAVLAG
                     WGGVFPLATGEVALSSGRPQNISRLLAFKLGSKSSLPPLPVFSRPQLKPPKPTASAAT
                     VQKGEALFQRYCSGCHGDVAVSGGVLPDLRYSGTLDNDQWFDIVLKGQLKQAGMVSFD
                     KELSRDDAAAIRAYVIFRANQSLAETKPAPK"
     gene            complement(16360..16650)
                     /locus_tag="VGR03_18505"
     CDS             complement(16360..16650)
                     /locus_tag="VGR03_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996332.1"
                     /translation="MACSSPALDCASGSGASEITQQVLLAGEIAGRSACSIAIRSQCV
                     SVWGVAHSLGCEEITAQAESGKGPNPTRPSAMKKLRILRIFYQSTTGPANGP"
     gene            16909..18198
                     /locus_tag="VGR03_18510"
     CDS             16909..18198
                     /locus_tag="VGR03_18510"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV1996333.1"
                     /translation="MKFRSGWILAIILAGLGACVAQGQEAPPSPPEKLADLLAEAERN
                     NPQIQAARHIVDAARQVPSQVSTLPDPQAVLQHVSVGSPRPFAGYTNSDFAYLGLGFS
                     QDIPYPGKLRLRGEIARKDADVAQQRYESIRRSIQSQIKSAYVQQSYLTKRIAILNGD
                     GEMLRQVEQAAEVRYRSGMGSQQEVLQAQIERTKLLREITMNELEAGKTQATLKQLLN
                     RSQSAPDIGTEPLSETATTQTYDELLNAAKANNPEIAGAREMIARQGLQVDLAKKDFY
                     PDFNLQYMWQRTDPVQFRAYYMITLGVRIPIYRSRRQQPELAQARADQDRARSESETQ
                     TQQIAFALRQEFLTQEKSAELLKIYREGLIPQAHAKLQAGMAAYQSNRQDFQALLASF
                     LDVLKLDGEYWQTLAEHETAWARIEEMTGLPLREGAR"
     gene            18211..>18638
                     /locus_tag="VGR03_18515"
     CDS             18211..>18638
                     /locus_tag="VGR03_18515"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV1996334.1"
                     /translation="MKNYRKAFLLALIGNLALLAILGGFWRHARKVPATQPPVVSAAA
                     PEQADQMKPAAATPAATEAPLAPIQLSVERLQSIGVKFGAVERKNVLDEIRVTGTVAI
                     DERRVSYVQTRFAGHIEMVFADATYQYVRKGQPLFTIHSP"
BASE COUNT         4120 a         4921 c         5759 g         3838 t
ORIGIN      
        1 gagcggagtc aggcctgctg gaagctgaaa tttcgtacag taaataggga tgaagccctt
       61 gggaagtacg ctttcggttt ttcgtgggtt agagggaaaa gggatgaaat cgggataaca
      121 cctggctttg gcatcacgca agtccggctt gacgacctgg ggcaggttca ataggtctag
      181 gcgtcgattg agcacctcca tattttccga ctcatgaaag attctccatg cctcggttcg
      241 cgtttcattc tccgcgttct gggcgttgaa caaaattctg taggcaggat cgtccgaagc
      301 ggctgcggcg ctaacctctg actgcgccct ctccgcctcg gctcgtcgaa aagttccgtt
      361 ctcttgaatg aaaaaccaga tcgaggcaaa gattgcgccg agcaaggcaa agatgacagc
      421 ttctcgggct atttttctca ttgcggtgaa acggaacagt acaccgcgga gcttcatgcg
      481 ggcaagcgtt gacttggggg aaagcgctcc cacactagtg aaatggagac tctcaaagta
      541 gggatggtca tgtcctcagt cactcagtgc tcgtatcaga ccgctgcttc gggaaggtgg
      601 tcccgcacag catggtaaaa tgagacaggg ggtaaacgac tatgagagag ttcggcttcc
      661 catgcaaaac gccgggttgt caggcctggc ttaaggttgg agacttgtta gatgattcgc
      721 ggcatgcaag tcacgttcct ataaatcgcg ctggttacga cccgcaaagg cttcagtgtc
      781 ctgattgcct gcaaacgtac gactactatt tttctgagaa ggaagttcga agagcggttc
      841 acaaacaaca cgcctgacgc agttgcagcc gcagctttct ccacctttct cgattcgctc
      901 caaaggcgga tgttacggct ccaggtccgt catcattgtt tgcaattagg cgaggtcggg
      961 gggattgccg ggtaccgcat tcctgccgtt tttgcaaggg tgcgggtgtt gacttctcct
     1021 caatcactcc acactgcgcc gcacaacgtc aggcattagg cggattcgac ggtgagccgc
     1081 aaaccgatgg ctttggcaac cgcaaccaat gtggacaatc gcgggctgcc gcgaatcgac
     1141 aaagccctat aaacactctc acgctcaaca ccagcagcct tggcgacttt ggcgataccc
     1201 cgcgcctgag caacgtgccg caaagcaatc agaagaacta ggggttcatc ctcatctttc
     1261 agcgccactt tcaggtactc tgcggcaaag ttcggatctt tccggagccg ccgaacgatg
     1321 gcctcgtcat gggaggcgct ggttttgcgt ttcatgaagt cccggccctc aattggattt
     1381 ctcattgtaa cttatacatc acactccgca aattgaaacc tctgccggtt tccgcacttt
     1441 cgtacctgtt ggtggccgcc tgaatttctt tctctgtttc tcgacaccac caggcgatca
     1501 gcgcgcctca tctatctgct tgactatata ttattgacga caaacggttt gacactatcc
     1561 aaggcagcca ctacactgcc cgtatggaag tgcatttcac tcccgagcaa gaagagcgcc
     1621 tctcgaagat tgccacgaag gaaggcgtcg acccccagaa actcgtcaag gatgcggcgt
     1681 tgcgcctgct ggaagacgac tcccgctttc gcgctggcgt ccgcaaaggg atcgaacagg
     1741 ctgaccgggg ccagttcgtc gaagaagaag aaatggacgc ccgcgtcaaa caaatgttcc
     1801 agccctgatg cgcttcagct ggacgctcgc tgccgcatcc gatttggagc acatcaagga
     1861 ctatctcacc gagcatcatc ctcagtttgc gcagcccacc gtacttgagc tttacgaaac
     1921 cattcgctct ctgaagacat cgccgcaccg tggtcgagtt gggcgcgaag aaggaactcg
     1981 cgaactggtg tttacccgcc tgccctatat tgtcgcgtac cgaataaagg atcagaccgt
     2041 cgaggtcctc cacattttcc acgctgccca gttccgcccc taaacttcac tcaagccacg
     2101 cgcgactgtt cagtggctcg cggcggcggc agtttcttct tttcgcgatt tgttccacag
     2161 ttgtatgtac ggctccaggt ccttcatcat tttttggaat tgtgggaggt ccaacgattg
     2221 cgcgccgtcg ctgatggcct tttccggcgc gggatgcact tccacgatta aaccatcggc
     2281 tccgatcgca acgcccgcgc ggcaaagcgc cggcaccagg ctacgcctcc ccgtcgcgtg
     2341 gctcggatcg agaatcaccg gcaagtgcgt caattcgttc agcgctggaa tcgccgcaat
     2401 gtcgcaggtg ttccgcgtca ccgtttcaaa cgtgcgaatt ccccgctcgc acaaaatcac
     2461 atcggaattt ccgtgcgccg taatatattc cgcggacatc aagagttctt tcaccgtgct
     2521 gctcaatccg cgtttcaata gcaccggccg gccgcatttt cccagcgcct tcagcagcat
     2581 gaaattctgc atgttgcgcg cgcccacctg catcacatcg gcgtagttcg ccaccagatc
     2641 cacgtgcacg tcgctcatca cttccgtgat aatcgccagc ccggtggcct ctttcgcctt
     2701 cttcagcagc ttcaaacctt cttcttccat cccttgaaaa tcgtatggcg aagtgcgcgg
     2761 cttgaacgcg ccgccgcgca gcatcttcgc gccggctttc gccacgccct cggccgcttg
     2821 catgatctgt ttttccgact ccaccgagca cggcccggcc atcacgacga attcactgcc
     2881 cccgatttcc gttccattca cttttatttt cgttttccca gcgcgaaatt ccttactcac
     2941 aaatttgtac ggggccgaaa tccgcaccac cttctccact tgcggcatgg cttcgacgga
     3001 ctcgaagcac gcggtcacat cccctacccc gacgacgccg atcaccacgc gctcttcccc
     3061 ttcgatggag tgcaccttgt atccgaagtg cgccacttgc tggcgaactt catcgatctg
     3121 ttctcgcgtg gcgtgcagct tcatggaaat aatcatggct tggtcctctc aacttccgtt
     3181 tttcttttct tcacaacttt tccccagaaa tactttccta aaacaaaaaa cccactcgct
     3241 gcgagtgggt cggatctttt ttctgctgaa tccgtttaca gaaacgcccc tcgccgccta
     3301 gttaggtgga ccgctaaatc gaaaccaata cgagtgggcg atgcaaatca tgcgcctagt
     3361 gtatttcgtc tctttcccgg acacaacaaa ttcttttagc aaaaaaggcc gccattcaat
     3421 tccataggga tccatgcggg tctcagaaaa atagaatcgc aatgcgatag cgcgagaatc
     3481 ccgccgctgc attcgcgcgg cacgccccca ccgccgcttt gctataaggt ccgctccgaa
     3541 tctttttttc cagatcttcc ggcaatttgt tcaggtctgc cacttcaaat cgcatcatcg
     3601 ctcttgccga cgtgcccgca aaccctgcat cggtctcgca tgtttttcgc gcgccgtccg
     3661 tcgcgttggc cgaccggaat ccctccgcgt tgagcaaaaa aatcactttc tgttcctgct
     3721 cctcaacact caattcgggg accaactgtg aaaaatcctg gacggcaaag aggccccccg
     3781 gcccgtgcac cgccgcgatg ccgacggagg tgagccgcgg gtccaggata ttctttcggt
     3841 ggccgggcga attcatccaa ccggcatgaa tgatctgggg attggggcca atggcgatgt
     3901 tctcggcgat gacactgaac cgcgcgccgg cttcggcgag gcggccctcc aggttggatt
     3961 cgctgggaag ttggtgctcg agcatattca gatcggccat gcggagggcg tggagacgcg
     4021 ccgctttgaa aagagcatcg tcccagtgga gagcagataa accctgggca gtgcgttcgc
     4081 ggttcagggc ctcgaagagc tggcgctccg agtcgttgcg ctgcaattgc gcgtttgcgc
     4141 tcgcagaaaa aaagaagaga aatgcggcgg ccaggagaaa tttggaattt ctcatgccgg
     4201 tgaaaatcag ccgcggacgc ggacggcgct aaagctgaga accacgtggc cgccggcagt
     4261 ggggcggccg aagctcagca tggggctgaa gcgggagaag agcagcatct ggtcaagctg
     4321 gcgccgaagt tccaagctgt ttgggccgga gagaatctga tagtcggtca tgtggccctg
     4381 ggcatcgacg gtgacgtcca gcaagaggcc gtgcggcaag gaaagttcga gatcgtcctg
     4441 ctccggcgcc caagcttgcg gatagtccga aagagaaacc agctcggcgg ggcgcatcag
     4501 attgagagtc acgtcgttct ggacggcgcg cacggtggtg cccggcccga tcatctggag
     4561 caccagcacg aaaacgagga tggcggagaa gaatccgccg gtggccggga cggtcagcgg
     4621 gcggaaaacg ttgtcgagga gaatttcggc gcgatccttg atgcgacgca tgcggctggg
     4681 ccaatcccgc aaatgctgcg cttgagcggc agcgaccttg atgcgcacgg cgagatcggc
     4741 aggcggaatg ttcttgggca cacgcgaaaa cataacggac agcttgcgga agcgttccaa
     4801 ctcctcgcgg caaacgttgc acccttccag gtgctgccga atctggacgc gctctgcaac
     4861 gcgcgcttca ccggttacag catcatcgag ataacccgcc agtttggttt ttaccgtgtt
     4921 gcacttcatt ggacaccctt gcccgtggaa cttgcccgcg gggacgtacc ttgcgaagaa
     4981 actgtaactc gccgcgcggc ggaaaatgca tcgccgaaag gcgacggctg cgactcggaa
     5041 acattgccat gcgaagacgt ttgcgagtga ataccgtgaa gctggtgtcc gcccaaaagc
     5101 ggctcaagaa tttctttcag cgcgcggcgc ccacgaagga tgcgggactt caccgtgcca
     5161 acgctgcatt ccagaacttc cgagacttcc tcgtaactca ggccttccag gtcgcgcaaa
     5221 atcaccgcgc tgcgaaaaac atcgggaatc tgttgtaaag cgccgtggac ggccacttga
     5281 atttccaaag cggcgagctg ctcgaatggg gtggcacaga gatcctcgac ttcgatgcgg
     5341 gcttgccctt cttccggttc ggcgtcgatg gaaacattct tctgcaggtg ccgcttgaac
     5401 cagcgcttgt gattcagtgc ctcgcgaatg gcgatgcggt agagccacgt tttcaaagag
     5461 ctgccctggc ggaaattgcg gatgccgcgg aaggccttca agaaaacctc ctggctaccg
     5521 tcggcggcat cggaagtgtc gccaagcatg ccgaggatga ggttatagac cggcgcatga
     5581 tagtgcgtga cgagccagtc gaaggccgtc tccgagccgg cttgcagctc gtggacgaga
     5641 tcggcttcat cgcggcccca agaaagggca ctagcccaat cggacactgc cggcctttcc
     5701 gactcgctgc tggaaagcga gccaccctac tacacggttc agatttagaa cgctgaacct
     5761 aggcacgact tgcaactccg accgggtcgg agtctgtgac cgggagcggt gtggaacagt
     5821 tcccgatatc cgctctcgta ttcttagaca ccggggggag cggagttgtt tccccggatg
     5881 gtgaagaatt cttgtgcctt tgcggattgt tgcaccaaag gcgtttggct gcaatgggac
     5941 ggatcgggta aacgttggaa cgtctgcggt ctgaacgtct gaacgtcgag ggaggtacac
     6001 ccccccccat gaaaatggat cgttatcaaa agaaaggagt tgcgtgagga gcattttgta
     6061 agtgcttgaa caaaaagggg ataattgatg gtaattttgg catgtttgca cgtttgaggg
     6121 ttggaatgtt tggcgtaccc ctacaccctc cgtcgcgatg gaaagagtgc ggaagttgtt
     6181 gaaagaaagg aagatacgtg tttcccgtgg agcaaaagag tgcgtaaacg tttgaaagga
     6241 aagggattga atcaaagttg aggaaaaggg actgagacag gggggcgcgg cagggggatt
     6301 aggtcggtgc tggggcttgg cgtacacggg agagaatagc atgccagtat caacttgtta
     6361 agtaaaatgc agtgcgtggg ggaaattgag ggttgaatgg ccgtggagac aggttgaacg
     6421 gctccggagg gtttggggcg aagtggaaaa aacggagtca atctgcccgc ggattagggc
     6481 ttgggtgttt ctttcgccgg ggttgaggcg ggggctgagg tcttggtgta gatgatcttc
     6541 ttcgtgccgc cgtcgcaagt ccctacgact ttgccttcgg cgtccttgtc tttcgccaca
     6601 atttccaggg tgtagccctt cacgcctttt gcgtcgagct tcttgctgat ttcatccttc
     6661 agctcttcac aagactttgc cgcttgtgcg tgagcgggca cggaaacaaa taatagaact
     6721 ccaattgccg ccagcagttt catggtgtga cctccatggt tgggatgccg gatcacaacg
     6781 gagcatacca tcggttcagc gcatccggcc gcccaccgcg caacaccttc gctctaaaag
     6841 atacgggata ggttcaagct gccacgcggc gctcgagccg gctttagatt attctaagca
     6901 gcgactttat gatgctatgg ttacgtccga ttcaggaggc ttccgtgcga cttgtgcttg
     6961 cggtggtggc atttagtgct atcttggcgg cccagaaact gggatcgaaa ggaacagaca
     7021 tggagatggc gcacaacgtt tcgataccgg aattgactga agcgatctca gcgcgactcg
     7081 tcgcccagtg ggagggctgg aagaaccagg atccggcacc caataatgcg atcatagccg
     7141 atgacttcaa ttctttctgg cccgatggct cccgccacat aggaaagccc acggcacagc
     7201 agatgtccga gcagcccatt acagggtaca aactgtcaca gtttcgcgtc gtgccggttg
     7261 gagcggatgg ggccctcgtc acctacttcg ccgacatcaa aacccccggc gacaacgtcg
     7321 agcatcacat ggccgtagga gaggtctggg tgaagcgcaa cggccagtgg ctaatccgcg
     7381 gttactcggg cacgctaata aagtagaccg ccaggaccgc ggcagctgct tcgatgtccc
     7441 gcccagcctt acgtgaacaa gagaaaagca aagtcaaaag ccccacgctt tcacaaaccg
     7501 aaagcttggg ccaccctgac cttgctcgct cagttaggct gtgccacccg cctggtggcg
     7561 atgtagacga tgatttttct gggagttttc atgaggcttc gcctcaagga cttttggggg
     7621 attataacgc tacggggagg gggcgtcatg tgtttggatt tctcgacgag caaagaaaga
     7681 aaattcaaaa cccccaccct ttcaaagagc aaacccgaaa gggcggggcg cccgctgaac
     7741 ggagaagaaa aaaccgaagt caaaaaccgc acccttaaaa gcgaagggtg cggcacccca
     7801 cccaagaaaa ggcaaaatcc tcacactcgc cgatcgggtc agggtaaacg ccaaccgagg
     7861 gcggggcacc cgccgctaca aaaaacaaga aggacggatg ggccagacgc acgagtggga
     7921 ttatgatgga agactatgtt gaaccgcacg aagagaacga tttgctgggc ggcgctggtg
     7981 ttgtgcttcg gggctaccga actgcgcggg cagaccgcgg cggaggctga cgatattccg
     8041 atcgagccct gcgacgtgct gcccgtggtg aaggtgcgca ttgatggggc ggagatgcgg
     8101 tttctgctgg acacgggcgc gacgacgatg ctcaacctga agtcgttcac cgcgggccgg
     8161 cgatgacgag cgcgcgagaa gtttcgctgc ccgaattggc gctgggaaat caccgcttgc
     8221 aaaacctaag gctgccggcg attgatttga cgccgatcgg gaaagcttgc gggaaacaga
     8281 ttgacgggat cctcggcgtg gacctgatgg acaagatggg tgtaacgatt gacttgaagc
     8341 ggcaagtggc ttcgctgggc gcggagtccc tggatgcgaa atgcgtgttc gaattgatgg
     8401 agaccgcgat gcaccactgc agcaccgcgt tcgagacggg gaatgccgtg gagcttgcgg
     8461 aatgcttcga cccggaaatc gttttctaca cgacgcaggg agaatatcgc gggcggaagc
     8521 aggtgatgga gtacctgagc cagcgctatt tgaaatttgc gccgaatctg cactacagaa
     8581 tgacgcttcg tgacgtgaaa accttcggcg atgccctgtg gtattcctac gattacgcga
     8641 tcgactctcc gcaagatcac atcgccggtc acggcatgtc catgtgccgg agagacggcg
     8701 gacggtggcg gatcctcaat ctccacaatt cgtgggtgga gggggcggct gccacaaaat
     8761 cagccgggca agaaaacatc caaaaacaat aaattcgttc tttgtgaagc gcagtccggc
     8821 gtacgggatc ggccgtgcgg aggcgcgtgc gggctggagc ttggctattg ggaatcggag
     8881 tccgattcgg aatcgaggcc ggcgcgggcg atgagattgc ggagggcttc ttcgccgccg
     8941 gcgccgcgct gggttccgac gatgaagccg tcgcggtcga tgacgacgta gatgggatag
     9001 gcggtggcgg catacatggc ggcgagattg gtgtcttcca tcatgacgat cttgcagttg
     9061 cgggggtgct gctcgagata ttttttgacg gttttcttgg actcgctgac gtcgatggcg
     9121 agaaggatga ggcctttgcc ggcgagctcg tggccgattt tgtcgacgaa ggcggcttcg
     9181 tcggcgcaga agccgcacca ggtggtccag aattcgagga ggacgacttt gcctttgatg
     9241 gattcgttgg tgaatttttc gccggtggtt gttgtggcgt tgaagcgggg ggcaggttct
     9301 ttgtccttgg gggcggcgag gacgcaaaga gaggcgagga tcaatacgga gagcgttgct
     9361 ccgcgcaaga ggatggttcg gggcaccatg ggggaaatgt aacagagtgg ggtcagcgcc
     9421 tgtcaattac tttattgtta aaaatcgagt gatgtcctgg gggatcgagg aagagtcaaa
     9481 taccccaccc tgcagaaaaa tgcttaacac agagttcaca gagagcaccc cgaccgggtc
     9541 ggggcaggcg ccgtacacgg agcggaaaga gaccgcgaaa gattgttcaa agttgctacg
     9601 ggacgcgaca ctaggaaccg gcacccgaat cgtgccgatt catttcaact cggccagccg
     9661 ccggttacgg gatgagcgtg ggacgtatgc ggccatgcag acgatagacg gagaaatcgc
     9721 gccggtccac ggtgaagatt tttcggaagc cttcgcgctc ggcaaccctg acgagtgcgg
     9781 catcggcgag atccatggga cgattggcgt acttgcgcat gagttcgcgg attcgcggag
     9841 cgtcggcgga gtcgagcgga aggagatgca gggcgccccg ctggatcatc tcccagaggg
     9901 cttcctgagc tttcgggagg tcagccaaga ggtacatggc ctcggttagc ggcggccaga
     9961 ccgtagccag gggctcgcgt aaggtcttga gcgccgcaac acacttggca tggtgctggt
    10021 catccgagtc gacgagggcc accagaggcc ccgcatccac aaggatcatc gagcgcatcg
    10081 ccgggggtcc cgcctgccct tgaggagttt cgtgaggctg cggcctgttt gctccgagcg
    10141 cttgggattt ccgccacgga ctacgccgat taagtcagcc atcacttcaa agggagaacc
    10201 gacaggttct tgatgctcgg cccaggcttc gatcgctcgg cgaattactt ccgacgtgga
    10261 gagcctcttg cggcgcgcga tgcgcgcgat ccgctggcga gtcttttcgt cgagccgcaa
    10321 agtgagaggg gaggccatag cgtcaccgca gggatgattg tagtgtatta cgatttgtaa
    10381 tacaagatgc cgggctaacg ttggtcacaa gcaagggaag gcagcagagg gcgcccgccg
    10441 cggcgggcgc ccctacacag agaagcccac acaattatag gagagcaacc tacttggagg
    10501 tgaggtagag aaagcgggag aggcgctgct cgcgggagag agtgtggacg caagagagga
    10561 aacgctggag gccgtcgaaa tttacctggc cttcgatggg gataaggata gattggcggc
    10621 gagcgcgacc gtcgtggcgg cgcttggcga tggaggcggc gttgggtgtg gtgtccacag
    10681 cgcggagaag atggaagccg gattgttcga ggagacgctc gttttcgccg cgggggacga
    10741 agaggtaatg gccgatggaa ctgcgcgtgg cgagctcttc gtcggagatg acgccggtga
    10801 ttacgagcgc atcgctgaaa aggaagcggc caccgggctt gagaatgcgg aggaattcgc
    10861 gaaggacggc gagacgatca ggaatatgac acatggcgtc attggaatag acggcgtcga
    10921 aggtggcgtc agcaaagggg agattggtgg cggcgttgtg aaggaggaag gtgacgcggg
    10981 attgcagcga tttcgattgc gccagggcgg ttgcgctctt gatgccttcg ggattgacgt
    11041 cgagcgcagt tacgcggcag ccggtggatt ccgcgaagtg gagggcgcag cctccggagc
    11101 cacaaccgac ttctagggcg gaggagtttt tcgagagttg caggaggccg gcaatttccg
    11161 tgaattcctc gggggaggcc cagttggatt ggccgaagtc cgcgccgtag gtttcgaggc
    11221 ggatttcgcg gtaggcgggg tgctcgaggt gcgagtagga agtgttgtac atgtcgactt
    11281 tttcgggaat ggggttggcg gacatgattg atcgtttgcg atggcggtcg gcgggagctt
    11341 aacaccggaa ttgaagaagg cggaaatttt ttggcgggaa gagtcgccaa ggcagacgcg
    11401 ggtagtattc ggggcgggcc tgtgttaaag aaggcccgca aggacaggag ggattatgcg
    11461 cggaaagatg atgaagttcg ggctcatcct actgttagtg gtgtttgtgg cgccggcatt
    11521 tggccaaggg caaccgccgg acaaaacgat cacggtgttt ggacaatcga ttcattattt
    11581 tgatctgggc tcgggaccgg tggtggtgct cctgcacggt ctggggagcc acaaagaaga
    11641 ttgggtgccg gtgctggagc cgatggcgca gaaataccga ttgctggttc ccgaccagat
    11701 tgggtttgga cgatcggaca agccgctgct ggattacagc attcaaacgt acgtggattt
    11761 tctgaatgag tttttgcggc aattgaaagt ggaaaaggcg agcctggtgg gcgaatcgct
    11821 ggggggtgga tcgccgggct ctacgtggcg gaaataagcg gcggcgcgca cttgataccg
    11881 gtggagaagc tcgtgcttgt agatgcggca gggctaaagc aggacaagcc gatcccggat
    11941 ttgaatccga gttcgctgtc ggcaatgcgt ggattgatgg aggcggtatt ctacgatacg
    12001 agctggttga atgaggaggc gctgcggaag attttcacag acaagctggc gacgaaggac
    12061 agctacaccg tgcgaagcat cttgagcaat ccggcgctcg gagcggaaag gctggacgac
    12121 cggctggtga gtatcaaggt gccgacgctg gtgacgtggg gaaaacaaga caaattgttg
    12181 ccgatcggag ccggagaacg atacgccgcg ggaatcgccg gggcgaagct ggtcagcttc
    12241 gagaagtgcg ggcatgttcc gccgattgaa aagacggaag agtttgttgc ggcggtgacg
    12301 gcatttcttg gtggcgggac gaccacccac tagaggaacg ctcatacgag catgcgaaag
    12361 aatagggttt gaacgaattg aaacgaaatt tgggagaacg gagtgagcgg aaacacggag
    12421 cgcagtcgca cgccggagtg gccatggccg gattcgctgg atgcgctgac ggcggcgccg
    12481 ggctatcacc ggttgctatt cgaaaacgag cgcgtgcgcg ttttggaagt gcggattagc
    12541 ccgggccagt ttgtgccggt gcacacgcat cgatggccga gtatggtgta tgtcgcgagc
    12601 agcagcgatt tcatccggcg ggacggcgag gggaaaccta ttcttgatag ccgcacgatg
    12661 gggcctccac ccgcgacgcc gacggcgcag tggacaccgc cgttgccgcc gtactcggtg
    12721 gaaaacatag gaaacgcgga aattcttctg atcaccacag aattaaaaga acaagcctta
    12781 gaacaaagag agactgactg tgctgcaaaa cgttgaactg atcgcttcct actggacgct
    12841 ggccggcgga ggcgagccgc atacggacag agagtacagc acgtttgatt tcaaggaccg
    12901 ggtagaagcc gcggccaaag ccggcttcaa gggagtcggg atctggcact cggacctgga
    12961 ccacatcctc aagaggagaa gcctgaagga gatgaagcgg attttggacg acaacgggat
    13021 gaaatacgtc gagctagagt ttttgcggga ttggtttgtg gatggagaac tgaagaagca
    13081 atcggacatg gagaggaaga aactgctcga agcatctcag gcgctgaacg caaaacacgt
    13141 gaaagtcgga gatttcgagc gcaagaaaac tccgatgccg agactgatcg aggcattcgc
    13201 ggagctttgc gtggaagccg ccaagtacag agcgaagatt ggtttcgaac tgatgccctt
    13261 tgggatgctt gatacgctga aggattccct gacgatgatc gaaggagcag gcgcaaagaa
    13321 cggcgggatc attttcgacc tgtggcatgt ggcgaagctg ggcattccct atgaggaagt
    13381 gagccgggtg ccgttgaaac acattgtcgg ggtggagatc aacgacggga ctttcacggc
    13441 gccgtggagc ctgcacgaag atacgatcaa ccatcgaagg ttttgcggcg aaggggagtt
    13501 tgacgtgaaa ggttttgtcg cggccatgga gaagaccggc tacgcgggcc cgtggggtat
    13561 tgaggttctc tcggcagaac tgcggcccag gccgctggag gaagctgtga cgaaggcatt
    13621 caaaacgacg atcgcgcaat tccgcgggta gcgcagtttc ctaaagtagt tggtagtgac
    13681 tcattttgaa aagagcttgt ttcttcgtcg agttagcacc ttgcagagat agccgacgat
    13741 gtactctctg cgcgcagttt cctccaggtc ttgattctca actaggtcac ggattcctat
    13801 acttagagct ttccaaagcc ccgtcttttc aaactctttg tagggatgtg gagagtcagt
    13861 tttacgctgc cgtcgcattg gaattctcct caagtagtca cgtcgcatgg catgaacagt
    13921 cacaagaatc gtcaaactga gtcgctaccc cgcagtccaa agcctattta cttgggcgct
    13981 ggttttgttt ccgcgagaga ttgattggcg cggaagatga cgtaagcgcg gatggcagcg
    14041 gcatcatcgc gcgagagttc cttgtcgaag gaaaccatgc cggcttgttt cagttggccc
    14101 ttcagtacga tatcgaacca ttgatcgttg tcgagcgtgc cggaatagcg cagatcgggc
    14161 agtacgccgc cgctgacggc gacatcgccg tggcaacccg agcagtagcg ttggaagagg
    14221 gcttcgccct tttgcaccgt agctgcgcta gcggtgggct ttggtggctt cagctgaggc
    14281 cgcgagaaca ctggcagcgg aggcaaactc gacttgctgc ctagtttgaa ggcaagcaat
    14341 cgacttatat tctgcggcct gccggatgag agcgcgactt ctcccgtggc gaggggaaac
    14401 acgccgcccc atcctgcgag aacggcgacg tactgttcgc cgttgacgga ataagtgatg
    14461 ggagcagcga ccacgccgct ttgtgcgtcg gcggaccaca gcttttgacc gttgtcggcg
    14521 cgataggctt cgaggtttcc ctgcgcggta ccctggaaga caagatttcc tgccgtggag
    14581 aggatgccgc cgtttacagg actctggcga tccacgcgcc atatttcctt ttgttgaaca
    14641 ggatcccaag cggaaagatg tcctttgatg gcgccgagaa tggctttctt gacctccggt
    14701 ttttgcggca tgccggcagc cacgaagtcg atgccggtgt tgaaagccag atcgctcggt
    14761 tgggaatgct ggtcggattt gtagaggaaa cctgagtcct ggacggggat gtaggctaag
    14821 cgcgtgatcc gactgaaaga catggagtgc caactgtgcc cgcccagtgg gccgggggcg
    14881 acgggtattc ccttcccttc cccgtagcgc gcttgggtgt tttcgacggg acggccggtt
    14941 ttcaaatcga tgccgctggc ccaagagacc gcggtatagg gcttcgccga gatgagttcg
    15001 ccggtgccac gatcgagaac gtagaagaaa ccattcttgg gtgcgtgaag aagaaccttt
    15061 cggggcgact ggttgatatt gagatcggcg agaacgatgg attcggcgga atcgaaatcc
    15121 caggcgtcac cgggagtttc ctgatagtgc cacacgtatt cgccggtttc cgctttgagg
    15181 gccacgaccg agcaagtgaa aagattatcc ccgccctggg ggctgcgggc gcggcggtcc
    15241 cagggagttc cgttgccaac gccaaagtag atgagatcga gatcggggtc gtagacgatg
    15301 gagtcccaaa ccgtgccacc gccgccaaat ttccgccact gaccggtcca ggttttcgca
    15361 gctttttcga gaatgggaga ctcgtacggc tgcgaaggat cgccggggac ggtgtagaaa
    15421 cgccagacca tttttccagt gttggcatcg taggcggaga tgtagccgcg cacgccgaat
    15481 tcagctccgc cattgccgat caacactttg cctttgacga cgcggggcgc gccggtgatg
    15541 gtgtaacggt attgcgggtc gatggtgaga gtttcccaga tgggttttcc cgaggcggcg
    15601 ttgagtgcga tgagacggcc atccaacgtg ccgacgaaaa cttttccctg ccaaacggca
    15661 acgccgcgat tgacgacatc gcagcaagca ttgatggccc actccggcgg cactttgggg
    15721 tcgtaggacc agagcttctc gcccgtagct gcattcaccg cgaagacttt gctccaagcg
    15781 gtggtgaaat acatcactcc atcgacgacg atcggagtgg cttcctggcc acgacgtgtg
    15841 tcgatatcta cgtaccacgc caatgcgagc tggccgacgt tttgatcgtt gatctgcttc
    15901 agcgggctga agcgctgctc gctgtaggtc cggccgtagc tcatccagtt tccgggttgc
    15961 tgatcggcat tcagcaggcg tgaggcgtct acattggccg gggaaaccgt ttcttgcgcg
    16021 gatgacggag aattcgattt cgagcgacaa cccgcggcga tcagaagcaa taaggtcact
    16081 gcaaggcttg gaaatgattt ccggggcatg aattctcctg agacgcgaat tggtggaaag
    16141 gttgcgccga ccgacgcagt tctttctctt acggcaaata ccagcttccg cgacctatct
    16201 atatacactt caacgctcac tcatgactag caaaacgagt ccaatggaag caggtatgtc
    16261 gaggtcgcaa agagtcaggg acgggggact tgtgacggcg gtcactatgc tcggtgacga
    16321 atctcctggc actcaaacgt caataggccg atgcttgcat caaggtccgt ttgctgggcc
    16381 tgtggtagac tgataaaaga tgcgcagaat ccttagcttc ttcatcgcgc tgggtctcgt
    16441 cggattgggg cccttgccgc tctccgcttg tgcggtgatt tcttcacagc cgagcgagtg
    16501 cgcgactccc caaacagaga cgcattgcga acggatggcg atggagcaag cggagcgtcc
    16561 tgcgatctcg ccggcgagca aaacctgttg cgtgatctct gaagcgccgc tgcccgaagc
    16621 gcaatccaat gctggtgaac tgcaggccac ggcaggttcc gctcttgacg cgagctctgc
    16681 cccgttgatt gtccgacatg agagcaagca gcctgttgag gcgctcgaag acttctcttc
    16741 ccctccgctt caaccactcc tctgtatctt tctgatctga acctttccta aaaagtgcct
    16801 ggtgtgcacg ggtgtctttc cgtgcacgca tccgcaatag agcaaaccgc ggacactcaa
    16861 tgctcggtag gaaatggggc tctgcccagg aaccggaaag gactcggaat gaaattcaga
    16921 tctggatgga ttcttgcaat cattttggca ggactgggag cgtgtgtggc gcagggtcag
    16981 gaggcgccgc caagcccgcc cgaaaaactc gctgacttgc tggcggaggc ggagcggaac
    17041 aatccgcaaa tacaagcagc gcggcacatc gtggatgcgg cgcggcaagt gccgtcgcag
    17101 gtttccacgc tccccgatcc gcaagcggtg ctgcagcacg taagcgtggg cagcccgcgc
    17161 ccgttcgcgg gctacaccaa tagcgacttc gcgtatctcg ggctgggctt ttcccaggac
    17221 attccctatc cgggaaagct gcggctgcgc ggagagatcg cgagaaaaga tgcggatgtg
    17281 gcgcaacaac ggtatgagtc catccgcaga tcgatccaga gccagatcaa gagcgcgtac
    17341 gtgcaacaga gttacctcac gaagcgaatt gcgattctca atggcgatgg tgagatgctg
    17401 cggcaggttg agcaagccgc cgaagtgcgc taccgcagcg ggatggggag ccagcaggaa
    17461 gtcctgcaag cgcagatcga gcgcacaaaa ctgctgcggg aaatcacgat gaacgagctg
    17521 gaggccggga aaacgcaagc gacgctcaaa cagcttctga atcgttcgca gtcggctccc
    17581 gatatcggga cggagccgct atccgaaacg gcgacgacgc aaacctacga cgaattgctg
    17641 aatgccgcga aagcgaacaa tccggagatc gcgggtgcaa gggaaatgat cgcgcggcaa
    17701 ggcctgcaag tggatctggc gaaaaaggat ttctacccgg atttcaatct gcaatacatg
    17761 tggcaacgaa ccgatcccgt gcaatttcga gcgtactaca tgatcacgct cggcgtgcga
    17821 attccgattt accgcagccg ccgccagcaa ccggagctgg cacaagcacg agcggaccag
    17881 gatcgcgcgc gcagcgaatc ggaaacgcaa acgcagcaga tcgcttttgc gttgcggcag
    17941 gaattcctga cgcaagagaa gtccgcggaa ctgctcaaaa tttaccgcga aggcttgatc
    18001 ccccaggcgc acgccaaact gcaggccgga atggctgcct accagtcgaa tcggcaggac
    18061 ttccaggcgc tgctggcttc ctttctggat gtactgaaat tggatggaga gtattggcaa
    18121 acgctagcgg agcatgaaac cgcctgggca cgcatagaag aaatgacggg attgccgctg
    18181 cgcgaaggcg cgagataagg ggacacagtt atgaaaaact accgcaaagc attcctgctc
    18241 gctctcattg gcaacctcgc gctcctggcg attctaggag gattctggcg gcatgcgcgt
    18301 aaagttccag cgactcaacc accggtggtc tcagcggctg cgccggagca agcagaccaa
    18361 atgaagcccg ccgccgcgac gccggccgcg acggaagctc cgcttgcacc gatccagctt
    18421 tcggtcgaac ggttgcagtc catcggcgtg aagttcggcg cggtggagcg gaagaatgtg
    18481 ctggacgaaa ttcgcgtgac cggcacggta gccatcgacg agagacgagt ttcctacgtt
    18541 caaaccagat tcgccgggca catcgaaatg gttttcgcgg atgccaccta tcagtacgtt
    18601 cggaaggggc agccgctgtt caccattcac agtccgga
//
LOCUS       DASYVO010000535         1759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_237721, whole genome shotgun sequence.
ACCESSION   DASYVO010000535 DASYVO010000000
VERSION     DASYVO010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237721"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VGR03_18520"
     CDS             complement(<1..606)
                     /locus_tag="VGR03_18520"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEV1996335.1"
                     /translation="MATFTGAIGTGSKWLLDKIVGAIAATGINPNFLTFLGLVVNFGA
                     AAFFAVGGFFTGALIIFFAGFLDMLDGQVARRQNRVTAFGAFYDSTLDRYADMALYMG
                     LLVYYSVSGRTPYVVLAAVATAGSVMVSYARARAESLIPLCKVGFMERPERLVLLIIG
                     GLFNRMGPVLWVIAVVSTITVIHRIIYTWQETKAGRILPGIK"
     gene            816..1502
                     /locus_tag="VGR03_18525"
     CDS             816..1502
                     /locus_tag="VGR03_18525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEV1996336.1"
                     /translation="MLTMTSEFSRRTFLLRAGTGLSAAWVSANWPALLSAAKHAHHAA
                     QAAQPPKLEFFTAEQAIEIDAISARIIPTDDMPGAREAGVVYFIDRGLTTFSADDQKT
                     YRGGLPELQSRVSEVFPNATKFSALTPEQQDEVLHTFDELAKPSQTAANSGQRAIRPR
                     PGAQNFFETLRQHTIAGFLIDPDYGGNHDGVGWKVIGREREHIFQAPFGYYDKDYPGW
                     QPVAKEADKK"
     gene            1499..>1759
                     /locus_tag="VGR03_18530"
     CDS             1499..>1759
                     /locus_tag="VGR03_18530"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEV1996337.1"
                     /translation="MTADQPHYRTSDEVDFVIIGSGAAGGILAKELSTNGFRVVVLEQ
                     GPHLTEADFTHNEIEILAQDKLTNHPKLQPNTFRKTPDEKAKP"
BASE COUNT          419 a          554 c          460 g          326 t
ORIGIN      
        1 tttgattccc ggaaggatgc ggccagcttt cgtttcctgc cacgtataaa tgatgcgatg
       61 aatgacggta atcgtcgaaa ccacggcgat cacccaaagc accggcccca tgcgattgaa
      121 aagcccgccg atgatgagca aaaccagccg ctcgggccgc tccatgaacc cgaccttgca
      181 caagggaatg agcgactccg cacgcgcgcg agcataactg accatcaccg aacccgccgt
      241 ggccaccgct gccagcacca cgtatggggt gcgcccgctc acggaatagt acacgagcaa
      301 gcccatgtac agcgccatgt ccgcataacg atccagcgtc gaatcgtaga atgcgccgaa
      361 ggccgtgacg cggttctgcc gccgcgcaac ctgcccatcc agcatgtcta agaaaccggc
      421 aaagaaaata atcagcgcgc cggtgaagaa tccgcccacg gcaaagaagg ctgccgcacc
      481 gaaattcacc accagcccga gaaacgtgag aaagttggga tttatccccg tggccgcgat
      541 ggcgccgaca atcttgtcga gcagccactt gctgccggtt ccgatagctc ctgtaaacgt
      601 agccatacct ttagtagtgt ctcaaaacgt ggctactttc gcgaaatagt ctgagttccc
      661 ctaattctgc ggcatcctat actagccgcc cagcacaccg caatcgacga ccggcgttgt
      721 tcaagtttcg atgcaagatt cgaatcactc gcatccgcca attcatcttg accgcgagcc
      781 tgcatcagcc tagagtttgc aagtttaagg atctaatgct gacgatgacc agcgagttca
      841 gccgtcgcac ttttcttctg cgtgcgggaa cgggcctcag tgccgcatgg gtttccgcga
      901 attggcccgc gcttctttct gctgcaaaac acgcccatca tgccgcgcaa gcggcacagc
      961 cgcccaaact ggaattcttc actgccgagc aagcgatcga gatcgatgcc atctcggctc
     1021 gcattattcc taccgacgac atgcccggag cgcgcgaagc tggcgtcgtc tatttcatcg
     1081 atcgtggcct gaccaccttt tccgccgacg atcagaaaac gtatcgggga ggcttgcccg
     1141 aactgcagtc ccgagtgagt gaggtgttcc cgaacgccac aaaattctct gccttgactc
     1201 ccgaacagca ggacgaagtt ctgcacacgt tcgatgagct ggcgaagccg tcccaaacag
     1261 cagccaactc cggccagcgc gcaatccggc cccgtcccgg cgcgcagaat ttctttgaaa
     1321 cgctccgcca gcatacgatc gccggattcc tgatcgaccc cgactacggc ggtaatcacg
     1381 atggggttgg ttggaaggtc atcggccgcg agcgcgaaca tatatttcag gcaccgttcg
     1441 ggtactacga caaagactat cccggctggc aaccggtggc gaaagaagcg gacaagaaat
     1501 gacggccgac cagcctcact acaggacttc cgacgaagtg gactttgtca tcatcggctc
     1561 cggtgcagcc ggtggcatcc tggccaagga gctttcgacc aacggctttc gcgttgttgt
     1621 tttggagcag ggtccgcacc tgacggaagc cgatttcacc cacaacgaaa tcgaaatcct
     1681 ggcacaggac aagctgacca accatccgaa gcttcagccg aacacgttcc gcaagacgcc
     1741 ggacgaaaaa gccaagccg
//
LOCUS       DASYVO010000536         8578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_193662, whole genome shotgun sequence.
ACCESSION   DASYVO010000536 DASYVO010000000
VERSION     DASYVO010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8578
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193662"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VGR03_18535"
     CDS             complement(<1..686)
                     /locus_tag="VGR03_18535"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV1996338.1"
                     /translation="MNRRTFIGTSIAATLATAAKPSWAADTAHQIDRVGVQLYTVRDA
                     MKTDFEGTIAKVAATGYKEVEFAGYFDHSPKDVRALLDKNGLSSPSCHVGYDVVEKKW
                     PETLEAAKIVGHSYIICPWIDEKQRKEPDGWKRAAELFNKAGEASKKAGIQFGYHNHS
                     FEFDPSDSLGGKLPYDFLLAETDAKLVAMEMDLCWISVAGKDPLAYFDKHPGRFPLVH
                     VKDWVKDASSS"
     gene            complement(789..4277)
                     /locus_tag="VGR03_18540"
     CDS             complement(789..4277)
                     /locus_tag="VGR03_18540"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEV1996339.1"
                     /translation="MWLGVLLVALALPLASRAQDTGYISGTVSDKSGAAIAGADVVVT
                     DAGGNLTRTTATNGDGAYVVAGLPGGAYNLFVTAKGFQKFSATKVVLDVAQKIRVDVQ
                     LTVGAITEVIEVTGESVAQVETQSSALSTTVTGQQVNQLELNGRNFTQLVTLTPGVIS
                     QTGSDDGVVGVYGNVAYSMNGGRTEYNNWELDGGDNMDNGSNATLNVYPNLEAIAEFK
                     VLTSNYGAQYGRNGSGTVEVETKSGTNAFHGSTFEYLRNEIFKAKSWAEGANPTAPKA
                     PYKKHDFGYTVGGPIFIPNHYNADKKKTFFFWSEEWRREKVSTTILQNVPSVAERGGD
                     FTDLCPGADCPSGSQVTANKVVPTAVGTALLAIIPTATPGLLNGGFPAVQQTVASPTT
                     WREELIRVDHNLTDNYRLTFRYIHDSWQTVVPNPLWGNGTSSFQNVTTNFLGPGTSFV
                     ARLNANISPTLLNEFVASYTGDHIVLTTGGPVGLPSGFAMGSLFNNGFGNKLPAVFLQ
                     TNAAYGTSFGQDTGYFPWHNANPTYTYRDNMTKISGKHIFQFGAYVVFAQKNEANSPN
                     VQGILTFDTSHASVTTGNAFADLLLGNIANYKQWSKETQYYDRYRIVEPYFQDDWRIS
                     KRLTLNLGVRVSLYGTYRERYKQAFNFEPSAFVVANAPGFANDGSLTATGNPFNGIIQ
                     CGGAGGTSAVPSSILLAYPSSSVGSSSNAGCLKGHLFNPAPRIGFAFDPKGDGKMAIR
                     GGYGIFYEHTNGNEGNTESLEGSAPLVLTSSQNNIAGGVGACPAGQSGYSCIGGSGSS
                     LFPFTPVSIPNKAVWPYVQQWNLNVQKELPSHIVLSAAYVGSKGTHLTLLSNANQIVP
                     LPASANPYKPGEAIGPNDCATLTTPSGVPVTGQALINLNVACGNVNVDAQRTNFRGFS
                     NINTLRDAANSIYHSLQVGAQRTLGDLTLSLSYTYSHSIDDSSDRSDNAFVNTYNIAA
                     NRASSNFDLRHNLSVSYVYALPFFKGSGFSHALLGGWQVSGITVAQTGTPFNVTFPTD
                     NAGVGNGTGTGARPDLVGNPNSGFSANQDPANRGPVFYNAAAFAAPRGLTFGNLGRNT
                     LNLPGRINFDFGVFKKFPINEKAGFDFRWETFNIFNHTQYNAISSTFAAGSGPFFHLS
                     GTHDPRRMQFGLRFYF"
     gene            4823..5920
                     /locus_tag="VGR03_18545"
     CDS             4823..5920
                     /locus_tag="VGR03_18545"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1996340.1"
                     /translation="MDPQASFAKGQTALQSGDLDAAEADFRQVLAADPRAGSAYSNLG
                     VIAMRRKDWDHAIVLLKKAEKLEPKMAGIRLNIGLVEYRRGNYAAAIAPLSSVLRDQP
                     DSQQARYLLGLCQVLTKKFAAAVTVLEPLWPEKSDDVLYLYLIDIAAVESGQKELDEK
                     ILSRMIAVGGGTPEFHLIMGKAFLNRYEVGEGKAELERAAEGNPDLPFLHLNLGITYM
                     RLGENERAEAEFRRDIALEPDLADNYEQLGVLYSRMQRDQDAEKSFREALDRDAKSAG
                     AYLGLAKLHQKQKKPQPALKMIDTALRLSPDIHGGHFLRGRILMQLGRQKEARAEFAA
                     AQKGMEGRLNKERESLEDDQVPNPELTRQPQ"
     gene            complement(5944..7347)
                     /locus_tag="VGR03_18550"
     CDS             complement(5944..7347)
                     /locus_tag="VGR03_18550"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV1996341.1"
                     /translation="MSFFPYGTLAEITRDIRSKNVSPVEIVELHLKRIETLQPKLNAF
                     VHLDAEGARLQARAAETSVLRGDPLGPLHGVPLSIKSCIDVAGWPCPSGSLLRKDYVP
                     KQDAPLVSRLKAAGAILLGNTNTPEFLMAYETDNRLTRKTSNPWNLTHSSGGSSGGEA
                     AAIASGCSAGGVGSDGGGSIRVPAHFCGICGLKPTPGRIPATGHFPPGAGAFSWIGVV
                     GPMARTIADVRLLFEVMAGPDAGDALSAPVPFRAYHDDELRGMRIGILESEALGAATP
                     ETRAAVERAAKSLGERGFTVEPFRLDGLDRALDLWWFFFGPVIGNLFQHSIVGHEDQI
                     SPMLREYLSYATFGDPITLDQFTKVCADRDLLRAEILRQMQDPPVLLSPVSTNPAFLH
                     GQGNYLPGTGYRDTMRFSQWLNLTGFPGATVPVGVSNDGLPIGVQVIGRPFEDELVLA
                     VAEAIEQARGPWQAPPI"
     gene            7602..>8578
                     /locus_tag="VGR03_18555"
     CDS             7602..>8578
                     /locus_tag="VGR03_18555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1996342.1"
                     /translation="MRSSGPPAQEKAAATKKSGSSGGGIADAYRFNTLGVAYMNQQRP
                     ADAQKYFDQALQADSKFAVARLNLGISLLAQQKLELARAALEAAAQQLPKDAYAWYNL
                     GLAHKDLGEPEKGIAAFQHVTEIAPNEADAYYFSGYLYSQLQKYDEAIAAFQKGLALN
                     AFHASSEFGLARAFQRKGDAAAAREHLARFQKITAEHLGAPFGAGYGDQGRYSLAELP
                     VSGIVEVPEAIPVRFTAQPNAFVSKSAGANHAGIGPSTGACFFDYDGDGKPDLFLVSG
                     VADGIGRLMHNLGDGRFEDVTSEAGIQLTGSGLGCAAGDFDNDGHTDLAV"
BASE COUNT         1821 a         2282 c         2682 g         1793 t
ORIGIN      
        1 gacgaagagg cgtccttcac ccagtctttc acatgaacga gcgggaaacg gccgggatgc
       61 ttgtcgaaat aggcgagagg atccttgccg gcgacgctaa tccagcagag gtccatttcc
      121 atggcgacga gtttggcgtc cgtttcggcg aggagaaaat cgtaagggag tttaccgccg
      181 agcgaatcag agggatcgaa ttcgaatgaa tgattgtgat agccgaactg gatgccggcc
      241 ttcttgctgg cttcgccagc tttattaaat agttccgcag cgcgcttcca gccgtccggt
      301 tctttgcgct gtttctcgtc gatccacggg caaatgatgt agctgtgccc gacgattttc
      361 gcggcttcga gagtctccgg ccactttttt tctacgacgt cgtagcccac gtggcaggaa
      421 ggcgaggaca agccattctt gtcgaggagg gcgcggacat ccttcggaga atggtcgaag
      481 tagccggcga attcgacttc cttgtagccg gtggcggcga ctttcgcgat ggtgccctcg
      541 aagtcggttt tcatggcgtc gcgcacggtg tacagttgca cgccgacgcg gtcgatttga
      601 tgtgcggtgt ctgcagccca ggatggcttg gcggcggttg cgagagttgc tgcgatggaa
      661 gtgccgataa atgtgcgtct attcatgggc cgcattttac tccttccagg agaaaaggga
      721 acctagattc ggggaggaaa agaagggcgg cgcttgttga aacgccgccc ttctggccag
      781 tgctctgatt agaagtagaa ccgcagaccg aactgcatgc ggcgtggatc gtgcgtgccg
      841 cttagatgga agaaggggcc ggagccggct gcaaatgttg agctgatggc gttgtactgg
      901 gtgtggttaa agatattaaa agtttcccag cggaaatcga agcccgcttt ctcgttaatc
      961 gggaacttct tgaagacgcc aaagtcgaag ttaatgcgtc cgggcaagtt cagagtgtta
     1021 cgtccgagat tgccgaaggt gagccccctt ggagcggcga aagccgccgc attatagaac
     1081 accggtccac ggttggctgg gtcctgattg gcggagaaac ctgaattggg gttgcccacc
     1141 aaatcgggac gggcgccggt acccgtgcca ttgcctacac ctgcgttgtc cgtgggaaag
     1201 gtcacgttaa agggtgtgcc ggtttgggcc acggtgatgc ccgaaacctg ccaaccgccg
     1261 aggagcgcat gggagaatcc actgcccttg aagaacggca aggcgtagac gtagctgacc
     1321 gaaagattat ggcgcaagtc gaaattcgaa ctggccctat tggctgcgat gttgtaggta
     1381 ttgacaaagg cgttatcgga tcggtccgag gaatcgtcaa tggagtggct gtaggtgtag
     1441 gaaaggctca aggtcagatc gccgagggtg cgttgtgctc caacctgcag ggagtgatag
     1501 atcgaattcg cggcgtccct aagggtgttg atgttactga atcccctgaa attggttcgt
     1561 tgcgcatcga cattgacgtt tccgcaagcg acgttgagat ttataagtgc ctgccctgtg
     1621 acgggcacac cgctcggcgt ggtcagagtt gcgcaatcgt tagggccgat ggcttcgcct
     1681 ggcttgtacg gattcgccga ggcaggcaaa ggaacgattt gattggcgtt gctgagcagg
     1741 gtcaaatgtg tacctttgct cccaacgtag gcggctgaaa gcacgatgtg ggagggcagt
     1801 tccttctgta cgtttaggtt ccactgctgg acgtaaggcc atacagcctt atttggtatg
     1861 gaaacgggcg tgaagggaaa gagcgaacta ccgctcccgc cgatgcagga atagccgctc
     1921 tgtccggcag gacaagcgcc gactccgcca gcaatgttgt tctgcgatga cgtgagcacc
     1981 aagggcgcag aaccttcgag cgattcggtg ttgccttcgt tgccgttggt gtgttcgtag
     2041 aagatgccgt agccgccgcg aatggccatc ttgccatcgc ccttagggtc aaatgcaaac
     2101 ccgatacggg gtgcagggtt gaagagatgt cccttcaaac agccggcatt cgaggaagag
     2161 cccacactag atgacggata tgcgagcaga atgcttgagg gcacggccga agttcctccg
     2221 gcccctccgc actggatgat gccgttgaac gggttgccgg ttgctgttag agacccatca
     2281 tttgcaaagc cgggcgcgtt ggctacgacg aatgcgctgg gctcaaagtt gaaggcctgc
     2341 ttgtaacgct cgcggtaggt tccatagagg ctgacgcgca cgccaagatt cagggtgagg
     2401 cgtttgctga tgcgccagtc atcctggaag taaggctcaa cgattctgta gcggtcgtaa
     2461 tactgtgtct ctttgctcca ctgcttataa ttggcaatat ttccaaggag aagatcggcg
     2521 aaggcattgc cggtggtgac ggacgcatgg gaggtgtcga aagtcaggat gccctggacg
     2581 ttgggagagt tcgcctcgtt cttttgggcg aacacgacgt aagcgccgaa ctggaagatg
     2641 tgcttaccag aaatcttggt catgttatcg cgataggtgt atgtggggtt ggcattatgc
     2701 cacgggaaat agccggtgtc ttggccgaaa ctggtaccgt aagcagcgtt ggtctgaagg
     2761 aaaacggccg gaagcttgtt tccgaaacca ttgttgaaaa gcgaacccat cgcgaaacca
     2821 gagggcagcc ccacgggtcc acctgttgtg aggacaatat ggtcaccagt gtagctggcc
     2881 acgaattcat tgagcagtgt cggcgaaata ttcgcgttca ggcgtgccac aaagcttgtc
     2941 cctggaccca ggaagttggt ggtgacgttc tgaaaacttg aggtgccatt gccccacagc
     3001 gggtttggca ccacggtctg ccaggagtcg tggatgtatc ggaacgtcag gcgatagttg
     3061 tcggtgaggt tgtgatccac gcggatcagt tcttcccgcc acgtggtcgg gcttgcaaca
     3121 gtttgctgga ctgccgggaa gccgccgttc aacagacctg gagtagcggt agggatgatg
     3181 gccagaaggg cagtgccaac cgcagttggc actactttat tggcagtgac ttgggaacca
     3241 ctcgggcagt cggcccccgg gcagaggtcg gtgaagtctc ctccgcgctc ggcgactgaa
     3301 ggaacgtttt gaagaatcgt ggtcgaaacc ttttcgcggc gccattcttc agaccagaag
     3361 aaaaaggtct ttttcttgtc cgcgttgtaa tggttgggaa tgaaaattgg ccctccaacg
     3421 gtgtagccga aatcatgctt cttgtaaggc gctttcgggg cggttggatt tgcgccttcc
     3481 gcccatgatt tcgccttgaa gatttcattg cgcaaatatt cgaatgtgct gccgtggaaa
     3541 gcgttagtgc ccgacttggt ttctacttcc accgtgcccg aaccattgcg gccgtactgc
     3601 gcaccgtagt tcgaggtcag aactttgaat tcagcgatgg cttcgagatt agggtaaacg
     3661 ttcagcgtag cgttgctgcc attgtccatg ttgtcgccgc cgtcgagctc ccaattgttg
     3721 tattcggtac ggccgccgtt catgctgtag gccacgttgc catagacccc gaccacaccg
     3781 tcgtccgagc cggtctggct gatgacgccc ggtgtaaggg tgacgagttg agtgaagttg
     3841 cggccgttga gctcgagctg attgacctgt tggccggtca cggtggtgct gagggcggaa
     3901 gattgcgttt ccacttgagc gacgctttcg ccggttacct cgattacttc agtaatcgcg
     3961 cctacggtca actgcacgtc gacgcggatc ttctgggcca cgtccagcac cactttggta
     4021 gccgagaatt tctggaatcc ttttgctgtc acaaaaaggt tataggcgcc accaggcaaa
     4081 ccagcgacga cgtaggcgcc atctccgttg gtcgcggtgg tgcgcgtcaa gttgcctccg
     4141 gcgtctgtaa ccaccacatc tgccccggca attgccgcgc cggacttgtc gcttaccgta
     4201 ccgctgatgt agccggtgtc ctgggcgcgc gaagcgaggg gaagggccag cgcaacgagc
     4261 agcacgccga gccacaacca cgcggctccc aaaatattct ttcgatgatt ggaaatgtta
     4321 gagttttcct tcacgttaag acctccaaga aagattcgaa ttttgggaga cgatggaatg
     4381 gagggacacg gcgcgaaagg ctacgacagc catcgttcga tttgccccgc gcagaaaacc
     4441 cctcatcgga caaagtcccc caccactcga cgaccgcagg caacgcaccg aaggtgtcta
     4501 ttaaaacgag ttaatagtgc atttaaacgt tgttgtcaag cgggaaattc atgccgaggt
     4561 gagatatagc acccgctgga gcggaacaag ggcgctatgc agcgttttat ggcgccagac
     4621 cgcagaggct tcttgaattc tcgctgggac tgccggacac taaatgttga tgcgcgcacg
     4681 attcttgaac tgccacaccc tggcatgcgt tctggcaatc ggtttccctc tggttgccgc
     4741 aaggtccgct tgtgcgaggc ctgcggcgga aggaggcgca gagcagcaat ccatctcttc
     4801 gccagccggt gcgaaccagc gaatggatcc ccaagcatcg tttgcgaaag ggcagacggc
     4861 gctgcagagc ggggacctcg acgcggcaga agcggacttt cgtcaggtgc tcgcggccga
     4921 cccgcgtgcc ggctccgcct attccaatct tggcgtcatc gccatgcggc gcaaagattg
     4981 ggatcacgcg atcgtgctgt tgaaaaaggc cgagaaactt gaaccgaaga tggctggaat
     5041 ccggctgaat atcggtctcg ttgagtaccg gcgcggcaat tacgccgccg cgattgcgcc
     5101 gctgtcctcc gtcttgcgcg accaaccgga ttcgcaacaa gcgcgttacc ttctggggct
     5161 ctgccaggtc cttacaaaga aatttgcagc agccgtgacg gtgctcgagc ccctttggcc
     5221 cgaaaagtcc gatgacgtgt tgtatctcta cctgatcgac atcgccgcgg tggagtccgg
     5281 ccaaaaagag ctcgacgaga aaatcctcag ccgcatgatc gctgtcggcg gaggaacgcc
     5341 ggaatttcac ctcatcatgg gaaaagcatt tctaaaccgc tacgaagttg gcgagggtaa
     5401 ggcggaactg gaacgcgccg ccgaggggaa tcctgacctt ccgttcctgc atctcaatct
     5461 gggcatcacc tatatgcgtt tgggtgagaa cgagcgggcc gaagcggagt ttcgccgcga
     5521 tatcgcgctg gaaccggatc tcgccgacaa ctacgaacag ctcggcgtgc tttattcgcg
     5581 tatgcaaagg gaccaggacg cggagaaatc atttcgcgag gcgctggatc gggatgcgaa
     5641 gagcgccggc gcatatctcg ggctcgcgaa gctgcatcaa aaacagaaaa agccacagcc
     5701 ggccttgaaa atgattgata ccgcgctgcg tctctcgccg gatatccatg gcggacactt
     5761 cctgcgagga cgaatcctga tgcaattggg ccggcagaag gaagcgcgcg cggagttcgc
     5821 ggccgcgcag aagggcatgg aaggccgctt gaataaggaa cgcgagtcgc ttgaggatga
     5881 tcaggtgccc aaccccgagc tgactcggca accgcaataa gagtcaccgc ctttcagaca
     5941 aaattaaatg ggaggcgctt gccagggtcc gcgggcctgc tcgatggctt ctgcgacggc
     6001 caggaccagc tcgtcttcaa acggtcggcc gatcacctgc acaccgatgg gcagtccgtc
     6061 gttcgaaacg ccgaccggca cggtcgcgcc aggaaagccg gtcaagttca gccattgcga
     6121 aaaccgcatg gtgtcgcggt atcccgtgcc ggggaggtaa tttccctggc cgtgaagaaa
     6181 cgcaggattc gtggaaacgg gagagagcag aaccggcggg tcctgcattt ggcgcagaat
     6241 ttcggcgcgg agaagatctc gatccgcgca gaccttcgtg aattgatcga gggtaatggg
     6301 atctccaaag gttgcatacg agagatactc gcgcagcatc gggctgattt gatcttcatg
     6361 gccgacgatg ctgtgctgga acaaattgcc gatcaccggg ccaaagaaaa accaccacag
     6421 atcgagcgcg cgatcgagtc catccaggcg gaaaggttcg acggtgaagc cgcgctcgcc
     6481 gagtgatttt gcggctcgct cgacggcggc gcgcgtctcc ggagttgccg ccccaagagc
     6541 ctcactctcg agaattccga tgcgcatgcc gcgcaactca tcgtcgtggt acgcgcgaaa
     6601 tggaacggga gcggagagtg catcgccggc gtccggtcca gccatcactt cgaagagcag
     6661 gcgcacatct gcaatggtgc gcgccatggg gccgaccacg ccgatccagg aaaaagcgcc
     6721 ggcgcccggc ggaaaatgtc ccgtagcagg aatgcggccg ggtgtgggct tcagcccgca
     6781 gattccacaa aaatgcgcgg gcacgcgaat cgagccgccg ccgtcgctgc caacaccgcc
     6841 cgccgaacat cctgaggcaa tcgctgccgc ttcacctccg ctcgagccac ctgaggagtg
     6901 cgtcaagttc cacgggttgc tcgtttttct ggtgagccgg ttgtcggttt cgtaggccat
     6961 caaaaattcg ggagtgttgg tattcccgag gaggatggcg cccgcagctt tcaatcgcga
     7021 aacgagcgga gcgtcctgtt tgggaacgta atctttgcgg agaagcgatc cggaggggca
     7081 gggccaaccg gcgacgtcaa tgcagctctt gatgctgagc ggtacaccgt gaagaggtcc
     7141 gagtggatcg ccgcgcaaaa ccgaagtttc cgccgcgcgc gcctgcaggc gggctccctc
     7201 ggcgtccagg tggacaaacg cattcagttt cggctgcagc gtctcgatgc gcttaagatg
     7261 gagttcgacg atttcgacgg gtgagacatt cttggatcga atatcccgtg tgatttccgc
     7321 taaggtgccg taggggaaga agctcatggc ttgatgcgcg agcgtaagcg gacaggcagc
     7381 gcaggtcaag cgcccggcga aatgcgcagt ttggcgcgca taataacggt tgacgaggtc
     7441 gatgacagcc gggagaattc caattgcgcc cggatgggtc gtacgcggaa ttcgggcatc
     7501 ccgcgcggac aggaagaaat attttcatgc attcaactta tcggcgagaa gcgggcgtta
     7561 ttttttcttg ttcactttta atttgctgtg ccgcgatatt ggtgcgctcc agcgggccgc
     7621 ccgcgcagga gaaggcggcg gctacaaaga agtctggatc atcaggcggc ggtattgcgg
     7681 acgcctatcg tttcaataca ctgggcgtcg cgtacatgaa ccagcagcgc cccgcggacg
     7741 cgcaaaaata cttcgaccaa gcactccagg ccgactccaa atttgctgtg gcccggttga
     7801 acctgggaat ttctctgctc gcccagcaga agctggagct ggcgcgcgcg gcgctcgaag
     7861 cggcagcgca gcagcttccc aaggatgctt acgcctggta caacctcggg cttgcgcaca
     7921 aggatctcgg ggagccggag aagggtatcg ccgcttttca gcacgtcacg gagatcgcgc
     7981 ccaacgaagc ggacgcctac tatttttccg gctacctgta ctcgcagttg cagaaatatg
     8041 acgaagccat cgcggcgttt cagaaggggc tggctctgaa tgcttttcat gcttcgtcgg
     8101 aattcggcct ggcgcgcgcg tttcagcgga agggcgatgc tgctgcggcg cgcgaacacc
     8161 tggcgcgctt ccagaaaatc accgcggagc atctcggcgc gccgttcggc gcgggctacg
     8221 gcgatcaagg ccgttactcg ctcgcggaat tgccggtgag cggtattgtc gaagtgccgg
     8281 aggcgattcc ggtgcgattt acggcccagc caaacgcgtt tgtttcgaag agcgcgggcg
     8341 caaatcacgc cgggatcgga ccgagcaccg gcgcatgctt cttcgattac gatggcgatg
     8401 ggaaacccga tcttttcctt gtgagcggcg tggcggacgg cataggccgg ctgatgcata
     8461 atctcggcga tggacgattt gaagatgtga cttccgaagc gggtatccaa ctcactggaa
     8521 gcggtttggg atgcgcggct ggtgatttcg acaatgacgg ccatacggac ctcgccgt
//
LOCUS       DASYVO010000537         3470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_11794, whole genome shotgun sequence.
ACCESSION   DASYVO010000537 DASYVO010000000
VERSION     DASYVO010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11794"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VGR03_18560"
     CDS             <1..312
                     /locus_tag="VGR03_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996343.1"
                     /translation="PTDTCAVLLTTAKLKQKSSPKDASPEDFKLHELRSMGTAKGTGE
                     IITYISLKSGLVVRATEETSQFMDVVVAKSDGSNHVHYNVEAKSHAEVLLVLETPLNR
                     P"
     gene            416..2464
                     /gene="uvrB"
                     /locus_tag="VGR03_18565"
     CDS             416..2464
                     /gene="uvrB"
                     /locus_tag="VGR03_18565"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEV1996344.1"
                     /translation="MNFKLHSNYQPRGDQPAAIEQLYRGVEAGEKHQVLLGVTGSGKT
                     FTMAKLIEQADRPALVMAHNKTLAAQLYHEFKGFFPENAVEYFVSYYDFYQPEAYVPS
                     ADIYIDKEATINDELDKLRMSATRSLFERRDVVIVASVSCIYGIGSPEAYYGMLITLE
                     KGQQTSREALLRRLVDIQYERSEDLRRGTFRVRGDMIEIYPPYQDEGFRLEMWGEQID
                     TIRQIDPLTGEVNSGQEDLPHVTIFPKTHYVMPQEQRDRAIVTIQEELWWWKKELEKQ
                     GKFVEAQRVVQRTMFDIEMMRTIGYCHGVENYSRHLSGRLPGEAPPTLLDYVPQDYLL
                     FIDESHQTVGQVRGMFNGDRARKQTLVDYGFRMPSALDNRPLTFEEFEHRINQVVYVS
                     ATPGPYELTKAGGVIVEQVIRPTGLVDPQVEIRPVKGQVDDLLEEIRVRSEKNERVLV
                     TTLTKRMAEDLSEYFTEIGVKCRYLHSEVETLDRIIILRDLRRGEFDVLIGINLLREG
                     LDLPEVSLVAILDADKEGFLRSTTSLIQTIGRCARHIEGRAILYADRRTDSMNQAIDE
                     TNRRRAKQVAYNKENNITPMSIIKSVDMELARIVEADYVTVPVDDASLDAATANIKSE
                     QQLAEMLQQLETQMREAAKKFEFEKAAQLRDRIRSLKQKDLAGLFSAESTPAPAEPAN
                     "
     gene            complement(2461..>3470)
                     /locus_tag="VGR03_18570"
     CDS             complement(2461..>3470)
                     /locus_tag="VGR03_18570"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1996345.1"
                     /translation="LFAARDEEEKLPAALATLMEIDYPGLEVIAVDDRSQDATGRILD
                     EFAAAHARLRVVHITQLPAGWLGKPHALLKAYESSTGEWLLFTDADVRFKSDVLRRAI
                     ALAKTRNLDHLTLMGDVEMVGFWETVLITFFGMAFHIATDPYRVTNPNSRAYIGVGAF
                     QLLRRSAYEASGTHRRLAMEVVDDMKLGKIVKQSGFRSGAGIAQDFVVVRWHAGLGNL
                     IRGVTKNFFAGVGYSVGLVAISITGLLLMNVAPIFGIFFGHGWIRIFAAIALVIALCF
                     HTGVDIGARVSPLYAFTHPLGAILFSYMLLRSTVVTLKQGGIIWRDTFYPLEDLKRGV
                     V"
BASE COUNT          822 a          948 c         1059 g          641 t
ORIGIN      
        1 cctaccgata cttgcgcggt gctgctcacg acggcaaagc tgaaacagaa atcgtcaccc
       61 aaagatgcgt cccccgaaga tttcaagctt cacgagttgc ggagcatggg aacggcgaaa
      121 ggcacgggcg agatcatcac ctacatttcc ctgaagagtg gtctcgtcgt gcgcgcaacg
      181 gaagagacca gccaattcat ggatgtcgtg gtcgccaaat ccgacggctc gaaccacgtg
      241 cactacaacg ttgaggcaaa gagtcacgcg gaagtgctct tggtcttgga aacgccgctg
      301 aatcgacctt aattcgaaac tgccgttacg tttccccata agaagccgca atttgcctcc
      361 cggagtataa tcaaggtttc ggcggcggcc ggcgaagctg caggagaccc ttcccatgaa
      421 tttcaagctg cacagcaatt atcaaccgcg cggtgaccaa cccgcggcga ttgagcagct
      481 ctatcgcggc gtggaagccg gagagaagca ccaggtgctc cttggcgtga ccgggtcggg
      541 caagacgttt accatggcga aactgatcga gcaggccgat cgtccggcgc tggtaatggc
      601 gcacaacaaa acactggcgg cgcagttgta tcacgagttc aaaggattct tcccggagaa
      661 cgccgtcgaa tatttcgtca gctactacga tttttaccag cccgaagcgt acgttccctc
      721 cgcggacatc tacatcgaca aggaagccac catcaacgac gagctagaca agctgcgcat
      781 gagcgcgacg cgttcccttt tcgagcgccg cgacgtcgtc atcgtggcct ccgtgtcctg
      841 catttacggc atcgggtcgc cggaagcgta ttacggaatg ctgattaccc tggaaaaggg
      901 gcaacaaacc tcgcgcgagg cgctcctgcg ccggctggtg gacattcagt acgagcggtc
      961 ggaggatttg cgacgcggca ctttccgcgt gcgcggcgac atgatcgaga tttatccgcc
     1021 ctatcaggac gaagggttcc ggcttgagat gtggggcgag cagatcgata cgattcggca
     1081 aatcgatccg ctgacgggcg aagtgaattc agggcaggaa gatctaccgc acgtgacgat
     1141 ttttccgaag acgcactacg tgatgccgca ggagcaaaga gaccgcgcga ttgtcaccat
     1201 tcaggaagag ctgtggtggt ggaaaaaaga actggagaag cagggaaagt ttgtcgaggc
     1261 gcagcgcgtg gtgcagcgga ccatgtttga cattgagatg atgcgcacca tcggctactg
     1321 ccacggtgtc gaaaattatt cgcggcattt gagcgggcgt ttgccggggg aagcgccgcc
     1381 gacgctgctg gattacgtgc cgcaggatta tttgttgttc atcgatgaat cgcaccagac
     1441 ggtagggcag gtgcgcggaa tgttcaacgg tgaccgcgcg cgcaagcaga cgctagtgga
     1501 ctacggcttt cgaatgcctt cggcgctgga taaccggccg ctgacgttcg aggaattcga
     1561 gcaccgcatc aatcaggtgg tatacgtctc cgcaacgccg gggccgtatg agctaaccaa
     1621 agccggcggg gtgatcgtgg agcaggtgat tcggccaacg gggctggtgg acccgcaggt
     1681 ggaaattcgt ccggtgaagg gccaggtgga tgaccttctg gaagagattc gcgtgcgctc
     1741 ggagaagaac gagcgcgtgc tggtgaccac gctgaccaag cgcatggcgg aggatttgtc
     1801 cgagtatttt acggagattg gcgtcaagtg ccgctacctg cactcggaag tggaaacgct
     1861 tgaccgcatc ataatcttgc gcgatttgcg acgcggtgaa tttgacgtgc tcatcggaat
     1921 caatttgctg cgcgagggtt tggatttgcc ggaagtgtcg ttggtggcca ttttggatgc
     1981 cgacaaagaa ggattcttgc gcagcacgac gtcgctgatc cagacgattg gacgctgcgc
     2041 ccggcacatc gagggccggg cgattttgta cgccgaccgg cgaaccgact cgatgaatca
     2101 agccatcgac gaaaccaacc gccgccgcgc caagcaagtc gcctacaaca aagagaacaa
     2161 catcacgccc atgtccatca tcaagtccgt ggatatggaa ctggcaagaa tcgtggaagc
     2221 cgactacgtg accgtgccgg tggacgacgc cagcctcgat gcggccacgg cgaacatcaa
     2281 gagcgaacag caactggcgg aaatgctgca acaactagaa acgcagatgc gcgaagccgc
     2341 gaaaaagttt gagtttgaaa aagcggcgca gctccgcgat cgcatccgct cgttaaagca
     2401 aaaggatctg gcgggactct tttcagccga atccactccc gcgccggcgg agccagcaaa
     2461 ctagacaacg ccgcgtttca ggtcctccag cggatagaag gtgtcacgcc aaatgatgcc
     2521 accctgcttg agcgtcacga cggttgagcg gagaagcata tagctgaaga gaatcgcacc
     2581 gagaggatgc gtaaacgcgt acagcggcga gactcgcgcg ccgatgtcca caccggtgtg
     2641 gaagcagagc gcgatgacca acgcaatcgc ggcgaaaatg cgaatccacc cgtggccgaa
     2701 gaaaataccg aagattggcg cgacgttcat gagcagaagg ccggtgatgg aaatcgccac
     2761 gagccccacg ctgtagccta cgccggcaaa gaaatttttc gtgacgccgc gaatcagatt
     2821 gccaaggccg gcgtgccacc ggacgacgac gaaatcctgc gcaattcctg cgccggaacg
     2881 aaagcctgat tgcttgacga tctttcccaa tttcatgtca tccacaactt ccatggccag
     2941 gcggcggtgc gttccgcttg cttcgtaggc ggaccgccgc agaagttgga aggcgccaac
     3001 tccgatatag gcgcgcgaat tgggattggt cacccgataa ggatcggtgg cgatgtgaaa
     3061 cgccatgcca aaaaaagtaa taagcacggt ctcccagaag ccaaccattt cgacatcgcc
     3121 catgagcgtg aggtggtcaa gattgcgcgt tttcgcaagc gcaatggcgc ggcgcaggac
     3181 atcggactta aaacgtacat cggcgtcggt gaacagcaac cactcgccag tcgagctttc
     3241 gtaggctttc agaagggcgt gaggtttgcc gagccagccg gcgggtagtt gcgtgatgtg
     3301 cacaacgcga agccgggcat gcgcggctgc gaactcgtca aggatgcgtc ccgtcgcatc
     3361 ctgcgaacgg tcgtccacgg cgatgacttc cagccctggg taatcgattt ccatcagcgt
     3421 ggcgagcgcg gcgggcagct tttcttcttc gtcgcgtgca gcgaagagaa
//
LOCUS       DASYVO010000538        18981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_11828, whole genome shotgun sequence.
ACCESSION   DASYVO010000538 DASYVO010000000
VERSION     DASYVO010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 18981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18981
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11828"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..2989)
                     /locus_tag="VGR03_18575"
     CDS             complement(20..2989)
                     /locus_tag="VGR03_18575"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEV1996346.1"
                     /translation="MEGRRVRFLLLVPVSIVLLMTYNARSQSRSTSAKKTAATEGAVP
                     AKSGVKKLSPAANAWVESTLRKMTVDEKIGQLLFTTYHGSLTATDTAAYGKITHDVTD
                     LHVGGFINITQGSPLGIVKSQAYPTAVLNNQLQAKSKLPLLIGADFERGTAMRLDEGT
                     SFPTAMALAAGGDVKDAYAMGKITALEARAVGIQWIYAPDADVNNNPGNPIINTRSFG
                     ENPERVAEFVSAFVLGVQENGGLATAKHFPGHGDTAADSHIDLPVIRADRARLDELEL
                     VPFRAAISLQVDSIMTGHLNVPALEPDPNTPATLSHNILTDLLRKQLGYQGLIVTDAM
                     DMGGITIRYAPGEAAVRAVVAGVDALLMPPVPDAAFEALQAAVKSGRISKERLDASVR
                     RILQAKARLGLNTSRLVDVNAINRKFGSAAWQKEAQEISDRGVTLLRDTPHRLPLDGT
                     KPSRTLLLAFYADPEPYPGEDLERELRSRFDSVTTLRADTRFVNASTLKLPSPESYDL
                     AILALFVRVSDRKGNVDVPAEQAALAEQIYKTGKPVITVGFGSPYLIERFPQAETWLA
                     AFGISDVAQISVARALFGQIPVRGHLPVTIPGVNLKAGFGIELPANPMDVQPMDVRGE
                     AQLQPAYEVIEKAIADKAFPGATLAVGYRGKVSVHAFGKLSYDAKAATTAANTMYDIA
                     SLTKVVGTTTLVAKLTEGDFPVPLDLDAKIERYLPEWASGPNTEWRHRVTVRHLLTHT
                     SGLPPFKEYWRTSKNKQDTLTKIFAEPLDYEPGTKEVYSDLGIILMAEIIERLTGRTL
                     DDLAKSCIFSPLAMKDTMYRPPKKLWPQTAPTEIDNNLRHRLVQGEVHDENAFAIGGV
                     SGHAGLFSTAPDLAAFCQMLLNGGVYAHQRILRRATIAQFTTPQQLSGGTRTLGWVVP
                     TEGGSSGHYFSAHSFGHTGFTGTSIWIDPDRQLFVVLLTNRVHPTRENTKIQQVRPAL
                     HDAVTQALGFASAAAPK"
     gene            3119..4363
                     /locus_tag="VGR03_18580"
     CDS             3119..4363
                     /locus_tag="VGR03_18580"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEV1996347.1"
                     /translation="MTGLKRAHIIFIHFTIIVALLSLGPGLAHAASKHSSHAARVQTG
                     LDVLEAQKFAPLRGKRVGLITNHTGLDSQGRSTVDVLSHAPGVHLIALFSPEHGLAGR
                     NDEKISSTKDPTTGLPVHSLYGETLRPTDEMLKGIDTLVFDVQDAGVRFYTYTTTMAY
                     CMEEAAKRNIAFFVLDRPNPLGGEIVEGPMLDADKTNFVGYFPLPVRYGLTIGELAQL
                     FNAENHIGADLHVIAMKNWHRNYFFESTGIKWIPPSPNLRTTKGSILYPGIEILQNAG
                     VSVGRGTQAPFEEFGAPWLNGDDVAAALNERHLPGLHFAAQPFIPIVGLYSGQRCGGV
                     AVRITDRSSVRSMRTGLEIAAILQKLYPKQFDVAKLLELTGNSDTIQQSQSGVAPEKI
                     VASWSSGMAAFEQVRRKYFLYK"
     gene            complement(4570..5199)
                     /locus_tag="VGR03_18585"
     CDS             complement(4570..5199)
                     /locus_tag="VGR03_18585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1996348.1"
                     /translation="MAQNENVKDPIQIVGICGSLRPGSFTRMALQIALRGARETGAEA
                     ALIDLREYQLIFCDGKEDENGYPKDVFRLREEVKRAQGLILGTPEYHGGVSGVLKNAL
                     DLMGFEELEGKMIGLVGVSGGALGAVHALSHLRTVGRALHAWVIPEQAAVSEAWKAFD
                     EKGNLKNPEIEKRLLDLGRQVARFSYLHSSEKALEFLRLWEGAPTNPGG"
     gene            complement(5250..6149)
                     /locus_tag="VGR03_18590"
     CDS             complement(5250..6149)
                     /locus_tag="VGR03_18590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1996349.1"
                     /translation="MKPSPRLQSALALAAAALTLLTPKAGAQGEKPSATKSGFVTSAD
                     GARIHYIDAGKGPAILFVPGWTMPGWIWEKQITQFAMTHRVVAMDPRAQGESSQTAEG
                     LYPAARARDIKAVTEQLKLAPVVLVGWSMGAIELAAYVDQYGTDTLAGLVFVDGWAGR
                     DFDPNTLPTMFQWVPGFQKGRRQWTEDFLHSSYMFKKPQPEGYLERLREAMLKTPTNS
                     AMAIWLGYITSDFRPALARIDKPTLIIAAEGGLCGSACEDMHKRIRGSQLQVIENVGH
                     ALFVEEPERFNSLLEDFVSGLAH"
     gene            complement(6244..6666)
                     /locus_tag="VGR03_18595"
     CDS             complement(6244..6666)
                     /locus_tag="VGR03_18595"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1996350.1"
                     /translation="MLQIDRVLETALYVNEMGRAIEFYQRIFRFPVLARSAEPDRLTA
                     LDIGGTQVLLLCKIGASVQAVVTPGGKIPPCDGSGNSHLAFPIALSELEAWEKHLEDN
                     GIVIESKVRWERGGKSLYFRDPDGNSLELATPGLWSTY"
     gene            complement(6740..7171)
                     /locus_tag="VGR03_18600"
     CDS             complement(6740..7171)
                     /locus_tag="VGR03_18600"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1996351.1"
                     /translation="MAVRLEHANLTVRDIEGMIRFLQTAFPEFRVRGEGKSRDGSRWL
                     HIGTDETYIALSPANAKFEKAWTPYRGLPGVNHLAYEVGDVESLCQRMKSAGYRESTP
                     TNAHPHRKRRYFYDSEGNDWEFVQYYSQDPAERNDYKLPDY"
     gene            7490..8434
                     /locus_tag="VGR03_18605"
     CDS             7490..8434
                     /locus_tag="VGR03_18605"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEV1996352.1"
                     /translation="MRIVSLLPSATEILFALGLDHEVVGVSHECDFPPRVRTKPVVIH
                     SRMPHDASPAEIDALVREYVHRGESLYAVDAEVLEALAPDLIITQDLCHVCAASPDDL
                     PTALARFDRRPEVLCLNPRDLGDVWRDILWVGEESCRGSQAEALLKQIGARLGALEQQ
                     LPQSADRPRVVFLEWLQPFYIGGHWVPEMIHRAGGEDVLGKVRTPSFRVHLQDVVEAE
                     PEILLIAPCGYSAQQARDEYRSMDFPEQWNAMPAVRNGRVYALEANSYFSRPGPRLIT
                     GMEALAKLLHPAIEVSREAEAAILPISANKPTSRAASV"
     gene            8534..8866
                     /locus_tag="VGR03_18610"
     CDS             8534..8866
                     /locus_tag="VGR03_18610"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1996353.1"
                     /translation="MIGSGIKRGTAELAVLSVLEGAPLHGYEMARRIEEQTKGALRFT
                     LAGLYPMLYRMEQRGWIRGAWETSNSGRRRRCYRLTPTGKKKLSPLRQEWSELFQALR
                     LLKKVSHA"
     gene            8931..9560
                     /locus_tag="VGR03_18615"
     CDS             8931..9560
                     /locus_tag="VGR03_18615"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996354.1"
                     /translation="MISELATHLEETWEELCKQGMTEEDAVRRTLSQVENWQDLRRRI
                     QAARKKENIMTNRVKQLWLPSLLTLLLSMGLLTLIQVFGPNPWLVARKSGWTLVAPVA
                     VIYIPWLLSLPLIGAMGAYLSNRAGGSRRAVFSSIVFPVLPYLVFFLLALPVMAIFND
                     QLAHNIMFGALFVGLFAWVLAPGAALFAGGMLAQFFLSRRLDSRRIVSN"
     gene            complement(9522..10955)
                     /locus_tag="VGR03_18620"
     CDS             complement(9522..10955)
                     /locus_tag="VGR03_18620"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV1996355.1"
                     /translation="MSEKRLELVRGLGAWASAAIVVGTMIGTGIFLKPAEMAREGRSV
                     SVVFAAWIIGAILSIFGAFSFAELGSMIPEAGGEYAYLRRGFGPVYGFLFGWTHSIVG
                     RPSSLASISAGMMRFLSFLFPIVAVPLFTVHITIPGLTGWIRPYDFVFTWAQPLAVVW
                     LLIMTGVNYLGVRLGGAVQVFLTSIKIMSVAIVIGVAFFSQAPAARAPDPFWPAAGMG
                     GTAIFSAFLATLAAALWAYDGWEDLNLVGSEVKDPQKNFPRALVGGVGFVAVVYLLFS
                     AACLKVLPFESVAASRHIASDVVEHIAGPTAAAWITLAMVISALGSMNSSVLSGARVG
                     YAMARDGIFFKIVDGIHPKFRTPGRSLLFQGVLASLMALTGTFEELTNLFIFAGWIFY
                     GFAVVALFRMRKTEPDLPRPYRCWGYPWVPGLFVAGALALTVNIWLERPGRSSIGLLL
                     ILAGLPFYRRWNRALSVANDSPGVQPS"
     gene            complement(10980..12443)
                     /locus_tag="VGR03_18625"
     CDS             complement(10980..12443)
                     /locus_tag="VGR03_18625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEV1996356.1"
                     /translation="MRIHPLDLAIVIAYLLGVTALGMRFRRGQQNATDYFLGGRTAPW
                     WALAFSIVATETSTLTIIGTPAIAYGGNLTFLQLVFGYLIGRVLIVLLLLPGYFRGEY
                     FTAYALIEKRFGKRMRAVAASTFLITRAIAEGVRVSAIALVVSVVLGTSERLAVVIVI
                     ALTVLYTFEGGMKAVIWTDVAQLLLYLTGSAVTFFVLLHRIPGGWSEVTQVAAAAGHK
                     LQLFDFSWNLATKYTFWSGLIGGAFLTMASHGTDQTIVQRLLAAKNERDSRRALLASG
                     AIVLFQFTVFLLVGVLLYVFAQHTPLLAAGERTDRILPLFLVREMPVGLAGLLLASIV
                     AVAMSNASGSLNSLAASSVLDFSKLRGQSADPARFLRLSRGMTLVWGLVLMGFGLVKW
                     GPLLLEKGLTVASLPFGSLLGLFLLGTLDHRANARGALAGMFAGLASVLYLYIYWRTH
                     VAFTWYVMIGASVTFVTGVVASRFSAGDKSREEKAPA"
     gene            12499..13512
                     /locus_tag="VGR03_18630"
     CDS             12499..13512
                     /locus_tag="VGR03_18630"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEV1996357.1"
                     /translation="MASRRLVKKSLRLASVFFVTPLRRIDWGSPFFTYALSMDYVLGF
                     DGGGTKTECVLMDPAGKILTRCFSGPSNPSRVGVESATEEIEKAADLCLHEAQVARNA
                     VAAIGAGLAGTGKPEMRERMRASLVGAFSGAAVSIFTDLEIALAAAGEGPVIVLVAGT
                     GSAAIGRNAQGQIWRTGGHGPRLGDDGSAFDIGSRAVARAMKERDQRGTDSILGMKIL
                     AQLGCASWTDLQERAALQPDKVFPSVFPIVAAAADAGDPAAREILLQAAGELSSLVNA
                     VAEHSGYGRENIMIVKTGGTVGRCAFFDMQLDAALKRVLPQAQIGGLRMSPAEAAARA
                     ARY"
     gene            13514..14341
                     /locus_tag="VGR03_18635"
     CDS             13514..14341
                     /locus_tag="VGR03_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996358.1"
                     /translation="MSQEGSVRERALVATGEELAVLLHHASAEVLLALLDNPALDETQ
                     VCMLLERKNLPGEVLEEVARRKPLLKSYRVKRSLAFHPRTPRLISLRLLRDLYLMDLV
                     QIAIVPGVSAELKRNAEDQLLARLPQLPLGQKITLARRGPARVAGALLAEGHTRVISI
                     VLDNPYMTEAQILRALSREMLPICVIPAVVQHRKWSITYNVRLALVRHPSAPLATILS
                     YLPELTVSDLRELAAPGIVPESLRKYLLAEVQRRMRASQKAATRSARSKDTPAPDRE"
     gene            14543..14881
                     /locus_tag="VGR03_18640"
     CDS             14543..14881
                     /locus_tag="VGR03_18640"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEV1996359.1"
                     /translation="MGSGDHPARRLERRWAPRYSFRADLEIEWGSAVLRGSTRDISAS
                     GMFIEAVDPLWVGAGFTARLTLDRPVRLDCFVTRIEPGRGMGVSVAVSESESQGLFEA
                     LLSSLSNTGS"
     gene            14903..15496
                     /locus_tag="VGR03_18645"
     CDS             14903..15496
                     /locus_tag="VGR03_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996360.1"
                     /translation="MTRCLDCGSERMADQCPSCGLTSAAAEVMFRRRLLKRTAVFLAG
                     SLGFPYVSQIYPPLDLDIMLVFFGVLFFVGLAIAVILDRRARNRRELEVLKRVYAGFI
                     PLPWIFAATLLVNGKLDSQKNMAYHPTTVDSRYNMPGIVRGTRRLFVHSWREGQKIER
                     LAVDFDDYDRFRAGDAVVVGVEPGALGIPWYYGVYRR"
     gene            complement(15621..16142)
                     /locus_tag="VGR03_18650"
     CDS             complement(15621..16142)
                     /locus_tag="VGR03_18650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996361.1"
                     /translation="MASLPYFVRNGVHVVDRSFDGMKPVQSIRAFTIACLLATTSAFL
                     VTAQTQSSEKKLLWKSVGFAIVKFNDEAPKSWNIYHTEKKGLLLVHLWKRYLLVDTKE
                     QEAYEIDPQTVKPAGENVEWSPTDKPDQPIETPDWKTRDIGSMQLVRFRLGKDGHVLE
                     LQVPLLINGKPAY"
     gene            complement(16482..17819)
                     /locus_tag="VGR03_18655"
     CDS             complement(16482..17819)
                     /locus_tag="VGR03_18655"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV1996362.1"
                     /translation="MTTPAHFVPASQLARSGRSEEVVAKHKKYLWPSVTNYFQQPLVA
                     DRGEMQHLWDVEGNKYLDFFGGILTVSVGHCNPKISSKVSAQVNRLQHTSTLYPNEHI
                     VALAEKIAQITPGNLQQSFFTNSGTEANEAAILLARMSTGSYDVVALRHAYSGGSALA
                     KAVTAHAPYRKGGVISVGISHAVNPYCYRCPLHLKYPDCEVACAGDVENLIQTGTSGN
                     IAAFIAEPIQGVGGFITPPKEYFKLVFKIVKKYGALFIADEVQTGWGRTGKKWFGIEQ
                     WEVIPDMITSAKGMANGVPVGLTVTTPEIAGGFQGANIATFGGNPVTCVAAKATIELI
                     EEENLLENADVVGTYFRGKLEELQAKYSLIGDVRGMGLMQGLELVKDRKTKEPAPEAA
                     TQVMERARQNGLLIGKGGLYGNTLRLAPMLNTTKPDVDEAIKLLDKSFSEVRN"
     gene            17982..18299
                     /locus_tag="VGR03_18660"
     CDS             17982..18299
                     /locus_tag="VGR03_18660"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HEV1996363.1"
                     /translation="MARFEEDANGREVSLRVGEVFEICLPETRTTGYKWAVEKSGEPV
                     CGLTGESADAPAGPPGRAGTHLWQFRAAQAGTATILLHHRRSWESGVAPGRIFQIHIQ
                     ATE"
     gene            complement(18356..>18981)
                     /locus_tag="VGR03_18665"
     CDS             complement(18356..>18981)
                     /locus_tag="VGR03_18665"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEV1996364.1"
                     /translation="QGQLGSCTGNAIGGAFQFEQMKQNIANWIPSRLFIYYNERVIEG
                     SVNSDNGAQIRDGVKSVATTGVCPETDWPYDITKFADKPPDPPCYADAAKNTATSYRR
                     VPQILNQMKGCLASGYPFVLGFSVYESFESPQVAQTGHAPMPHTGEKQIGGHAVLAVG
                     YEDAHHWFIVRNSWGPGWGMAGYFTIPYSYLTDSNLADDFWTIRLVA"
BASE COUNT         3894 a         5706 c         5400 g         3981 t
ORIGIN      
        1 aacgctgatt ctattggggt tacttcggag ccgctgcgga agcaaaccct agcgcctgcg
       61 tcaccgcgtc atgcagcgcc ggacgcactt gctgaatctt tgtattttcc cgcgtcggat
      121 gcacgcggtt cgtcagcaac accacaaaaa gctgccggtc cggatcgatc cagatcgaag
      181 tcccggtaaa tcccgtatgc ccaaaactgt gcgccgaaaa ataatgcccg ctcgacccgc
      241 cttcggtggg caccacccag cccagcgtcc gtgtgccgcc ggaaagctgc tgcggcgtcg
      301 taaattgcgc aatcgtcgcg cgccgcaaaa ttctctggtg cgcgtatacg ccgccgttca
      361 gcaacatctg gcagaacgcc gccaggtccg gcgccgtgct gaaaagtccc gcatgccccg
      421 aaacgccgcc gatggcaaac gcgttctcat cgtgaacttc gccctgcacc aaccggtgcc
      481 gcaaattatt gtcgatctcc gtcggcgcgg tctgcggcca tagttttttc ggtggacggt
      541 acatcgtgtc cttcatcgcc agcggcgaga aaatgcagct cttcgccaag tcgtcgagcg
      601 ttctccccgt caaacgttcg atgatctccg ccatcaaaat aattcccaag tccgaataca
      661 cttccttcgt ccccggctcg tagtccagcg gctccgcgaa aatcttcgtc agcgtgtcct
      721 gcttgttctt cgacgtccgc cagtattcct tgaacggcgg caatcccgaa gtgtgcgtca
      781 gcaaatgccg caccgtgact ctgtgccgcc actcggtgtt cggcccgctc gcccactccg
      841 gcaaataccg ctcgatcttc gcatccaagt cgagcggaac aggaaagtcg ccctcggtta
      901 atttcgccac cagtgtcgtt gtccctacca ctttcgtcag cgacgcgatg tcatacatcg
      961 tattcgccgc ggtagtcgcg gctttcgcgt cgtaacttaa ttttccgaac gcgtgcaccg
     1021 aaacttttcc gcgataaccc accgccagcg tcgcgccagg aaacgccttg tccgcaatcg
     1081 ctttctcgat cacttcataa gccggctgca attgtgcttc gccgcgcacg tccatcggct
     1141 gcacgtccat cggattcgca ggcaattcaa ttccaaatcc agccttcaag ttcacgccgg
     1201 gaatcgtgac gggcagatgc ccgcgcacgg gaatttgtcc gaacagcgcc cgcgccaccg
     1261 aaatctgcgc gacgtcgctg attccgaacg ccgccagcca cgtctccgcc tgcggaaacc
     1321 gttcaatcaa atacggactt ccgaatccca cagtgatcac cggcttgccc gtcttgtaaa
     1381 tctgctcggc cagcgccgcc tgttccgcgg gcacatccac atttcctttg cgatcgctca
     1441 cgcgcacaaa cagcgccaga atcgcgaggt cgtaactttc cggcgacggc aatttcagag
     1501 tagaagcatt cacaaaacgc gtgtccgccc gcagcgtagt caccgagtcg aatcgcgaac
     1561 gcaactcgcg ctccaaatct tcgcctggat aaggttccgg atcagcataa aatgccagca
     1621 gaagcgtgcg cgacggcttg gttccatcga gcggcagccg gtgaggtgtg tcgcgcaaca
     1681 gtgtcacacc gcggtccgaa atttcctgcg cttctttctg ccaggcagcg ctgccgaatt
     1741 tccgattgat cgcattcaca tcgaccagcc ggctggtgtt cagtccaagc cgagctttcg
     1801 cttgcagaat tcgccgcacc gacgcatcga gacgttcttt cgaaattcgt cccgacttca
     1861 ccgccgcttg caacgcttca aaagcggcgt ccggcaccgg cggcatcagc agcgcatcca
     1921 ctcctgcgac aaccgcgcgc accgccgctt ctccaggtgc gtaccgtatt gtgatcccgc
     1981 ccatgtccat cgcgtccgta acgatgaggc cctgataccc aagttgtttg cgcagcagat
     2041 cggtgaggat gttgtgcgaa agcgtagccg gcgtattggg atcgggctcg agagcaggaa
     2101 cgtttagatg ccccgtcatg atgctgtcga cttgcaagga aatagcagca cggaagggaa
     2161 caagctcgag ttcatcaagg cgagcgcggt ccgcgcgaat cactggcaga tcgatatgcg
     2221 agtcagcggc cgtatcgcca tggcctggaa aatgtttggc cgtcgccagt ccgccgtttt
     2281 cctgcacgcc aagcacaaac gcgctgacga attccgcaac acgctccggg ttttcaccga
     2341 aggatcgcgt gttgatgatc ggatttccag gattattgtt tacatccgcg tccggcgcat
     2401 agatccattg aattccgacg gcgcgcgctt cgagcgccgt gattttgccc atggcgtacg
     2461 cgtcttttac atcaccgcct gctgcgagcg ccatcgccgt tggaaaagac gtgccttcat
     2521 ccagccgcat ggccgtgcca cgctcgaaat ccgcgccaat cagcagcggc agtttcgact
     2581 tggcctggag ctggttgttt agcactgccg tgggatatgc ctggctcttc acgattccca
     2641 gcggcgatcc ctgcgtaata ttgatgaatc ctcccacgtg gagatcggtc acgtcgtgcg
     2701 tgatcttgcc gtaggctgcc gtgtccgtgg ccgtcaaact tccgtgataa gtggtgaaaa
     2761 gaagttgccc aatcttttcg tccacggtca tcttgcgcag cgtggattcc acccaggcgt
     2821 tcgcggcggg cgacaatttt ttcaccccgc tctttgcggg aactgcgcct tccgtggcgg
     2881 cggttttttt tgccgaagtg ctgcggctct ggctgcgtgc gttataggtc ataaggagaa
     2941 cgattgagac gggaaccagg agcaaaaagc ggacgcggcg tccttccatc aattagaatc
     3001 ccccagcggc cgccagttgt ctgtatttat acatagcaca cgcacatggt gctctcaacg
     3061 cggcttcggc cagcgcgttc cagaatatca ttgtatggtt tcaatagcgg ggaacccttt
     3121 gactggactc aagcgcgcgc acatcatttt tatccatttc acgatcattg tcgcgcttct
     3181 ttcgctggga cctgggttgg cgcacgctgc atcgaaacat tcaagtcacg cggcgcgtgt
     3241 gcagaccggt ctcgatgttc tcgaagcgca gaaattcgcg cctcttcgcg gcaaacgcgt
     3301 cggcctcatc accaatcaca ccggcctcga ttcgcaaggg cgcagcaccg ttgatgtttt
     3361 atcgcatgcc cccggggtgc acctgatcgc tctattcagt ccagaacacg gtctcgccgg
     3421 ccgcaacgat gaaaagatct cttccaccaa agatcccacc accggtctgc ccgttcatag
     3481 tctttatggc gaaacgctcc gtcccacgga tgaaatgctc aagggcattg acacgctcgt
     3541 ctttgacgtt caggacgccg gcgtccggtt ttacacgtac acgacaacga tggcttattg
     3601 catggaggaa gccgccaaac gcaacatcgc cttcttcgtc ctcgatcgtc ccaatccgct
     3661 cggcggtgaa atcgtggaag ggccgatgct cgacgccgac aagactaatt tcgtcggata
     3721 ttttccattg ccggtgcgtt acggcctcac gattggcgaa ctcgcgcaac tcttcaacgc
     3781 ggaaaatcac atcggcgccg accttcacgt cattgccatg aagaactggc atcgcaacta
     3841 ttttttcgaa agcaccggca tcaagtggat tccgccctcg ccgaatcttc gcaccaccaa
     3901 gggttccatt ctctaccctg gcattgaaat cctgcagaac gccggtgtct ccgtcggccg
     3961 cgggacccaa gcgccgtttg aagaatttgg cgctccctgg ctcaatgggg acgatgtcgc
     4021 cgcggcgctg aacgagcgtc atcttcccgg cttgcatttt gccgcgcaac ccttcatccc
     4081 catcgtcggg ctctacagcg gccaacgttg cggcggcgtg gcggtgcgca tcaccgatcg
     4141 ctcctccgtg cgctccatgc gcacaggcct ggaaatcgcc gccatcttgc agaagctcta
     4201 cccaaaacaa tttgatgtcg cgaagctgct cgagctgacc gggaactccg atacgattca
     4261 gcaatcgcag tcgggcgtgg cgcctgaaaa gatcgtcgca agctggtcca gcggcatggc
     4321 cgccttcgag caagtccgcc ggaaatattt tctctacaaa tagttccact gtatgtgcct
     4381 gacagtgcgc cggcagcgta gcactgcgtc aagaggacga atggtggcca ataggaaagg
     4441 aagcaagaaa actcaagccc cgacaaccta tcggcgcaaa ttcatagtgc tcaactccgc
     4501 cgtctcatga cctttatcaa ttgggactca atcaggccga ttccgactgt tatgcacttc
     4561 ctcgaaccct tatccgcccg ggttcgtagg cgcgccttcc cagaggcgca ggaattcgag
     4621 agctttctca gagctatgca gatagctgaa tcgggccacc tggcgtccca aatcgagcag
     4681 ccgcttctct atttcggggt ttttcagatt tcccttctca tcgaatgctt tccaagcttc
     4741 agaaacggca gcttgctcgg gaatgaccca ggcgtggagc gcacggccga cggttcgcag
     4801 atgacttagg gcgtgaactg cgcccagagc tccgccggaa acgccgacga ggccgatcat
     4861 ctttccttcc agctcctcaa aacccatcag gtcgagtgcg ttcttcaaga cgccgctcac
     4921 cccgccgtga tattccggag tcccgagaat cagtccttga gcgcgtttga cctcctcccg
     4981 cagtcggaaa acgtccttcg gatacccgtt ctcgtcttct ttcccatcgc agaagatcag
     5041 ctgatactcc ctaagatcaa ttaatgccgc ctcagcgccg gtttcacggg cgccccgcag
     5101 ggcaatctgc agggccatcc gcgtaaagct acccgggcga aggcttccgc aaatgcctac
     5161 gatctggatc gggtctttta cattttcgtt ctgggccatg ttaaccgtct ccgaggtttt
     5221 gacacaaagg ctcgcgccgg cctaatcgct caatgggcga ggccgctaac gaagtcttcc
     5281 agcagcgagt tgaagcgttc cggttcctcc acaaaaaggg cgtggccgac gttttcgatt
     5341 acctgcagct gcgagccgcg tatgcgcttg tgcatatcct cacaggcgct tccgcagagg
     5401 cctccctcag ccgcgatgat caatgtgggt ttatcgattc ttgccaaggc tggccggaag
     5461 tcagaggtga tatatccgag ccagatcgcc attgctgaat tggttggcgt tttaagcatg
     5521 gcctccctca gtcgctctaa gtagccctcc ggttgtggct tcttgaacat atagctcgaa
     5581 tggaggaaat cctcggtcca ctggcgccgt cctttctgga agccaggcac ccattggaac
     5641 atggtcggca gcgtgttcgg atcgaagtcg cgcccggccc aaccatccac gaacaccaac
     5701 cccgccaatg tatcggtgcc gtattgatcc acgtaagcgg ccaactcaat cgctcccatt
     5761 gaccagccaa ccaaaaccac gggggcgagc ttgagctgct ccgtgacagc cttaatgtcg
     5821 cgcgcccgcg cggcggggta gaggccttcg gcagtttgcg aagattcacc ctgcgcgcgc
     5881 gggtccattg caacgacacg atgtgtcatc gcaaattgag tgatttgctt ttcccagatc
     5941 cagcccggca tggtccagcc gggcacaaac agaatggccg gcccttttcc cgcatcgatg
     6001 taatgaattc ttgcgccgtc cgcagaggtg acgaagccgc tcttcgtcgc cgatggtttc
     6061 tcgccttgag cgccagcctt cggcgtaagc aaggtcagcg cagcagccgc cagcgcaagt
     6121 gcgctttgca aacgagggct cggcttcatt ttcaacctcc ggcaagatga aagcccgttg
     6181 acaatctgaa ccccgacgca gtccattcca gttaaccgcc ttcagggaag gataaaagca
     6241 agctcagtag gtcgaccaga gacctggcgt ggccaactcc aacgagtttc catcaggatc
     6301 gcggaaatag aggcttttgc cgccacgctc ccaccgcacc ttgctttcga tcacaattcc
     6361 attgtcttcg agatgtttct cccatgcttc cagttctgaa agtgcaatcg gaaaagccag
     6421 atgcgagttg ccgctgccgt cgcaaggagg aatcttgcct cccggtgtca ccacggcttg
     6481 caccgaggcg ccgatcttgc atagcagcaa gacctgcgtc ccgccaatat caagcgccgt
     6541 caggcggtcg ggctccgccg atctcgctaa cacgggaaac ctgaagatgc gctgatagaa
     6601 ctcgatggcg cggcccattt cgttaacata tagggcggtc tcaaggacgc gatcgatctg
     6661 caacatgcca gtgactcctt tcttggaaaa gtgcccgccg ggtccgctgc aaccgttggt
     6721 taggtccttc gaaggtgtgc tagtaatccg gcaacttata atcgttgcgc tcagcgggat
     6781 cttgagagta gtactgcacg aactcccaat cgttgccctc ggagtcgtag aagtaccggc
     6841 gcttgcggtg cggatgtgcg ttggtcggcg tggactctct gtatccggct gacttcattc
     6901 gttggcacaa actttcgacg tctccgacct cataagcaag gtgattcact cccggcaggc
     6961 cccggtacgg tgtccaggct ttctcaaact tcgcgttggc tggactcaag gcaatgtagg
     7021 tttcatccgt gcctatatgt agccatcggg agccgtcccg acttttgcct tcgccacgca
     7081 cccgaaactc aggaaacgcg gtttgcaaga aacggatcat cccctcgatg tcacgtacag
     7141 tgaggttcgc atgttcgagc cggacagcca ttgcacacct ccaatgcggc ttaacccttg
     7201 gtcagactag cctggatgag atcgggccca gttgttgtaa gtaaccactg tcgcttctgt
     7261 ggcatgacac cttcggaggg aatgccggag atcgtgcgcg tttccatagg ctgccaagcg
     7321 gggagagagc aacagcagaa agaatcaagt gtcccgcagc ttagcacttt cttgagttcg
     7381 cagctatcaa ttccgggaca atcagctgac acgaaggaag gggctccgcg ttacctattt
     7441 cacagttcga cttcccgcag cacggtctac agtggtacat tcctgtccca tgcgcatcgt
     7501 ttcgctgctc ccgtcggcca cagagattct ctttgcgctc ggcttggacc acgaagtcgt
     7561 gggcgtcagc cacgaatgtg actttccacc gcgcgtccgc acgaagcccg tggtcattca
     7621 ttcgcgcatg ccacacgatg cttcgccggc ggaaatcgac gccctcgttc gcgagtacgt
     7681 acaccgcggc gaaagtttgt acgccgtcga tgccgaggtg ctggaggcgt tggctccgga
     7741 tctgatcatc acccaggatt tgtgccatgt ttgcgccgcc tctcccgacg atctacctac
     7801 ggctctagcg cgattcgatc ggcgccccga agttctctgc ctcaatccgc gggatttggg
     7861 cgatgtgtgg cgcgacatcc tttgggtggg agaagaatcc tgccgcggtt cgcaggcgga
     7921 agcgctgctg aagcagatcg gtgcgcgtct cggcgcgctg gagcagcaat tgccgcaatc
     7981 ggcggatcgt cctcgcgttg tcttcctcga atggctgcaa ccgttctaca ttggcggcca
     8041 ctgggttccg gaaatgatcc accgtgcagg cggggaagac gttttgggca aagtccgcac
     8101 gccttcgttc cgcgttcatt tgcaggatgt cgtcgaggct gaaccggaaa ttcttcttat
     8161 cgcgccttgc ggctacagcg cgcagcaggc acgcgacgaa tatcgctcga tggattttcc
     8221 cgagcagtgg aacgcaatgc ccgccgtgcg caacgggcgc gtctacgcat tggaagcgaa
     8281 tagctatttt tcgcggcccg gcccgcgtct gatcaccggc atggaagccc ttgcgaaact
     8341 cttacatccc gccattgaag tgagccgcga agccgaagcc gccatcctgc caatctcagc
     8401 gaacaagccg acgtcccgcg ccgcctccgt gtagtcccgc cgccagggag gttttccttt
     8461 gggcacctga tctcacggac agttgacaaa ttcccgttca tacataagaa tcttatacgt
     8521 aagggagcaa cctatgattg gttcaggaat caagcgcggc accgcggaac tcgccgtcct
     8581 cagcgttctg gagggcgctc cgctgcacgg ctatgagatg gcgcggcgca tcgaggagca
     8641 aaccaaggga gccctgcgct tcacgcttgc tggcctctac cccatgcttt accgcatgga
     8701 gcagcgcggc tggattcgcg gcgcctggga aactagcaat agcggccgcc ggcgacgctg
     8761 ctatcgactc actcctaccg ggaagaagaa gctttcgccg cttcgtcagg aatggtcgga
     8821 acttttccag gcgttgcggc tgctcaagaa ggtgtctcat gcgtgattgg gaggcgctcg
     8881 tccggcgcaa acttgctggg atcgtcctca agcccgacga atgcagtgaa gtgatttcag
     8941 aattggcaac tcatctcgaa gagacttggg aagaattatg caagcaggga atgacggaag
     9001 aagatgccgt ccggcgcacc ttatctcaag tcgagaattg gcaggatttg cgtcgaagga
     9061 ttcaagccgc ccgtaagaag gagaacatca tgaccaaccg cgtcaagcaa ttgtggttgc
     9121 ccagcctgtt gaccctattg ctgtcgatgg gtcttctcac gctcatccag gtttttggcc
     9181 ccaacccgtg gctggtcgcc cggaagagcg gttggactct cgtcgctccc gtggcggtaa
     9241 tttacattcc atggctgctt tcgttgcctt tgattggtgc aatgggagcg tatctgtcaa
     9301 atcgggcggg cggctcacga cgcgcggtgt tctcatccat cgtatttccc gtcctgccct
     9361 atttggtctt tttcctgttg gcgcttcctg tgatggcaat ctttaatgat caactcgccc
     9421 acaacatcat gttcggtgcc ttgttcgtcg gcttattcgc ctgggtgctg gccccgggag
     9481 cggcgctttt tgcaggcggg atgcttgcac agttctttct ttcacgacgg ttggactccc
     9541 ggcgaatcgt tagcaactga aagcgcccgg ttccaacggc gatagaaggg aagtcccgca
     9601 agaatcagga gcaagccaat cgaggagcgg ccggggcgct ccagccagat atttaccgtc
     9661 agcgccagcg cgcctgccac gaacaggccg gggacccagg gatagcccca gcagcgatag
     9721 gggcggggca aatcgggttc ggtcttgcgc atgcgaaaga gcgcgaccac ggcgaaacca
     9781 taaaagatcc agccggcaaa aataaaaaga ttggtcagct cttcgaatgt tcccgtaagc
     9841 gccatcaggc ttgcgagcac accctgaaag agcagggaac gtccgggcgt tcggaacttt
     9901 gggtggatgc catcaacaat cttgaaaaat attccgtcgc gggccatagc gtagccgacg
     9961 cgcgccccgc tgagcacgga tgagttcatg gaacccagcg cggagatcac catcgcgagc
    10021 gtgatccatg ccgccgccgt gggtcccgcg atatgttcta caacatccga cgcaatgtgc
    10081 cgcgacgcgg ctacgctttc gaatggcaac accttcaggc atgccgcgct gaagagcaga
    10141 taaacgacgg cgacaaatcc gactccgccg accagcgcgc gcgggaaatt cttctgcgga
    10201 tctttaacct ctgacccgac aagattcaaa tcttcccatc cgtcataagc ccaaagcgcc
    10261 gcggccagag tggccagaaa cgcgctgaag attgcggttc ctcccatgcc cgccgccggc
    10321 cagaacggat ctggcgcgcg cgccgcgggt gcctgtgaaa aaaacgctac gccgatcaca
    10381 atcgccaccg acatgatctt gatggaggtc aaaaacactt gtaccgcgcc gcccagtcgc
    10441 acgccgaggt aattcacgcc cgtcatgatg agcagccaga cgactgccag cggctgcgcc
    10501 caggtaaaaa caaaatcgta gggtctgatc cagccggtga gcccggggat cgtgatgtgc
    10561 actgtgaaaa gaggcaccgc gacaattgga aaaaggaaac tgagaaaccg catcatgccc
    10621 gccgagatgg acgccaggga actggggcgt cccacgatcg aatgcgtcca tccgaagaga
    10681 aatccgtaga ccgggccgaa gccgcggcgg agataagcgt attcgccacc ggcttccggg
    10741 atcatcgaac ccaattcggc gaaggaaaat gcgccgaaga tggaaagaat cgcgccgatg
    10801 atccaagcgg caaaaacgac ggagacgctg cgtccttcgc gcgccatttc cgcgggcttc
    10861 aggaaaatgc ccgtgccgat catggtgccg acaacgatgg cagcggaggc ccacgcgccc
    10921 agcccgcgca ccagttccag ccgtttttcg ctcatcgctc ctcggtagcg gtggccttct
    10981 taggccggcg ccttttcttc tcgcgactta tcaccggcgc tgaatcggct tgcgacaacg
    11041 ccagtcacaa acgtgacgct cgcaccaatc atcacgtacc acgtaaacgc cacatgcgtt
    11101 cgccaataaa tgtaaagata gagcacgctc gcgagtccag caaacattcc tgctagcgcg
    11161 ccacgcgcat tggcgcggtg atccagggtc cccagcagaa agaggccgag caggctgccg
    11221 aagggaagcg aagccaccgt cagccctttt tccagcagca gcggtcccca tttcaccagg
    11281 ccgaagccca tgagcaccag tccccacacg agcgtcatac cgcgggagag ccgcagaaag
    11341 cgggcagggt cggcactctg tccgcgcagt tttgaaaaat caagcacgct tgaggccgca
    11401 agggaattca acgacccgct ggcgttggac atcgccacgg ccacgatcga agccagcagc
    11461 aggcccgcaa gtcctaccgg catttcgcgc acaagaaaaa gcggcagaat gcggtctgtg
    11521 cgttctcctg cggccagcag cggcgtgtgt tgcgcgaaga cgtagagcag aacgccaacc
    11581 agcagaaaca cggtgaactg gaacagaacg atggcgccgc tcgcgagcag agcccgccga
    11641 ctgtctcgct cgtttttcgc cgccaacagc cgctgcacga tggtttggtc cgtgccgtgg
    11701 ctggccatgg taagaaacgc gccgccgatc agcccggacc agaacgtata tttggtcgcg
    11761 agattccagg aaaaatcgaa aagctgcaat ttgtgccccg ccgcggccgc cacttgtgtc
    11821 acctcgctcc agccgccggg aatccggtgc agcaaaacaa aaaacgtcac tgcgctaccg
    11881 gtcagataga gcagcaactg cgccacgtcc gtccaaatca ccgctttcat cccgccttcg
    11941 aaggtgtaca agacggtgag cgcgatgacg atgacgaccg ccagcctctc cgaagttccc
    12001 agcacaacgc taaccactaa tgcaatcgcc gaaacgcgca cgccttcggc aatggcgcgc
    12061 gtgatgagaa acgtggaagc agcgacggcg cgcatgcgct tgccaaagcg cttttcgatc
    12121 agcgcatacg cagtgaaata ctcgccacga aaatatccgg gtagcagcaa caagacgatg
    12181 agaacgcggc cgatcaaata gccgaaaacc agttgaagga acgtcaggtt tccgccatag
    12241 gcaattgccg gcgtcccgat gatcgtgagt gtggaggttt ccgtggcgac gatcgaaaac
    12301 gccagcgccc accacggcgc tgtgcggccg cctagaaaat agtccgttgc gttttgctgc
    12361 ccgcggcgga aacgcatccc cagcgccgta acgccgagca ggtacgcaat gacaatggca
    12421 agatcgagcg gatgaatgcg catcgagtgt cttttccact gcgcatttca ggtgcggcgg
    12481 aaaagtgcgt cgaggatagt ggcctcccgt cgcctcgtca agaagtcgct tcgcttggcg
    12541 tcagtatttt tcgtaacccc cttgcggcga atcgattggg gttcgccgtt tttcacgtat
    12601 gctctttcca tggattacgt tctgggcttc gatggtggcg gcacaaagac ggaatgtgtc
    12661 ttgatggacc cggcgggaaa gattcttacc cgctgctttt ctgggccatc gaacccttca
    12721 cgcgtgggtg tggaatcggc caccgaggaa atcgagaaag cggcggatct ttgcttgcac
    12781 gaagcacaag tcgcacgaaa tgcggtcgca gcgataggtg cgggattggc gggtacgggg
    12841 aagccggaga tgagggagcg gatgcgggca tcgctggtag gagcgttctc cggcgcggcg
    12901 gtcagcattt ttacagattt ggaaatagcg ctggcggctg caggagaagg cccggtgatc
    12961 gtcctcgtcg cgggaaccgg atctgctgcg attggccgga atgcgcaagg ccaaatctgg
    13021 cgcacaggag gacacggccc gcggctcggt gacgacggca gtgcgtttga catcggcagc
    13081 cgggcggttg caagagcgat gaaagaacga gaccagcggg gaacggactc gattctggga
    13141 atgaagattc tagcgcaact cggttgtgcc tcctggacgg atctgcaaga gcgcgccgcc
    13201 ctgcagccgg acaaggtttt tccttcggta tttcccatcg tggctgctgc ggccgatgcc
    13261 ggagatccgg cagcgcgtga gattttgctt caagcagcgg gcgaattatc atctttggtg
    13321 aatgccgttg ccgaacattc gggctacggc cgcgagaata ttatgattgt gaaaacggga
    13381 ggaaccgtag gccggtgcgc attttttgat atgcaacttg acgcggcgtt gaagcgggtc
    13441 ttgccccagg cgcaaatcgg cgggcttcga atgtcccccg cggaggcagc ggcacgcgcc
    13501 gcgcgatatt gaaatgtccc aagaaggatc agtccgcgaa cgcgctctcg ttgccacggg
    13561 cgaagaactc gccgttctgc tgcaccatgc ctcggcggag gtgcttctcg cgctgctcga
    13621 taatccggcc ctcgacgaaa cgcaggtttg catgctgctg gagagaaaaa accttccagg
    13681 agaagttctt gaggaggtgg cgcggcgcaa accacttctg aaaagctatc gggtgaagcg
    13741 ttcgctggct tttcatccgc gcacgccgcg actgatcagc ctgcgcctcc tccgcgacct
    13801 ctatttgatg gacctggtgc agattgcgat tgtgcccggt gtttccgccg agctgaagcg
    13861 caacgccgaa gatcaattgc ttgcacgcct gccgcaactt ccgctcggcc agaaaatcac
    13921 attggcgcgg cgcggcccgg cgcgcgtggc cggcgcgttg ctcgccgaag gtcacactcg
    13981 agtcatctcg atcgttctcg ataatccgta catgacggaa gcgcaaatcc tgagagcgct
    14041 ctcccgcgaa atgcttccta tttgtgtgat tccggccgtc gttcagcacc gcaagtggtc
    14101 gatcacctac aacgtccgcc tcgctcttgt ccgccaccct tcggcgccgc tcgccacaat
    14161 tctttcctac ctccccgagc tcaccgtctc cgacctgcgg gaattggccg ccccgggcat
    14221 cgttccggag agccttcgca agtatctcct ggccgaagtg cagcgccgca tgcgcgccag
    14281 ccaaaaagcc gcaactcgga gcgcccgttc caaggatact cccgcgcctg accgggagta
    14341 agtatccgct ttccattcgt acgattcgaa tgtcacctgt gtgacttcct tggcatcgaa
    14401 aatcacgttg ctgggaacaa aacatgcgtc tccggcgtcg tatgtgttgt ggtggaagaa
    14461 ggcgtatgct ggcaaccgag aaccggcgat tccgcccgga cgccgcggca cgtaatccct
    14521 ggcagggggt tgcaccagac ctatgggcag tggggatcat cccgcccggc ggctcgaacg
    14581 ccgctgggcg ccgcgttatt cgttccgcgc cgacctagaa atcgaatggg gctccgcggt
    14641 gcttcgtgga agcacgcgcg acatcagcgc cagcggaatg tttatcgaag ccgtcgatcc
    14701 gctctgggtg ggcgccggct tcactgcgcg actgactcta gatcgtcccg taagactcga
    14761 ctgtttcgtg acacggatcg agccggggcg cggcatgggc gtatccgtgg ccgtatccga
    14821 gtccgaaagc caggggcttt tcgaggccct gctttcctca ctctccaaca ccggctctta
    14881 atcccgtttt atactgaatg caatgacacg ctgccttgat tgcggttccg agcggatggc
    14941 ggaccagtgt ccctcctgcg ggctgacttc ggcagccgcc gaagtcatgt tccgccggcg
    15001 actgctgaag cgcaccgcgg tatttctcgc cggttcgctg ggatttcctt acgtcagtca
    15061 gatttatcca ccgctcgatc tggacatcat gctggtgttc ttcggcgtgc ttttctttgt
    15121 cgggctggcc atcgccgtga tcctcgaccg ccgcgcgcga aatcgccggg aactcgaagt
    15181 gctcaagcgc gtgtacgcgg gatttattcc gctgccgtgg atttttgccg ctacacttct
    15241 tgtgaacgga aagctggact cgcaaaaaaa catggcctat caccctacta ccgtggatag
    15301 ccgctacaac atgccgggaa tcgtccgtgg cacgcgccgc ctctttgttc attcctggcg
    15361 cgaagggcag aaaatcgagc ggttagcggt ggatttcgat gactacgatc gctttcgcgc
    15421 cggcgatgcg gtggttgtgg gcgtcgaacc gggcgcgctc ggcattccct ggtattacgg
    15481 cgtgtatcgc cgataatctt accgccgcca tactcattcc ctgtagctca ccccttcagg
    15541 ggtgaggctt ttctggtccg cggcttgacg ccgacctcaa agcaaaagcc tcaggcctga
    15601 aggcctgagc tacacctcat tcagtacgcc ggtttgccgt tgataagaag cgggacttgc
    15661 agctccagga cgtggccatc ctttccgagg cgaaatctca ccagttgcat gctgccaata
    15721 tcgcgcgttt tccaatcggg agtttcaatc ggttgatcgg gcttatcggt tggtgaccat
    15781 tcgacgttct cgccagcggg cttcaccgtt tggggatcga tttcgtaggc ctcttgctcc
    15841 ttcgtatcga ccagcagata ccgcttccac aggtgtacca ggagcagccc ttttttctcc
    15901 gtgtgataga tgttccagga cttcggcgct tcgtcattga attttacaat ggcaaacccc
    15961 acggatttcc aaagcagctt cttctcgctc gattgtgttt gagcagttac aaggaaagcg
    16021 ctcgtagtgg caagaaggca ggcaatcgta aaggcgcgaa tagattgaac cggcttcatt
    16081 ccatcgaagc tgcgatcaac cacatgaaca ccattgcgga cgaaataagg caagctggcc
    16141 atctcaattt tcccttgcct ggcagtccaa atacaaatcc accgagaccc aggagcaatc
    16201 ccacgccata acaccgtaga agtatcggat cgtaataggg gaatcctcct cgtgccactg
    16261 accagattac cagaacgaac cacagcgcga cggaaagccc gcccaggctg aatgcggcga
    16321 aagccagttg gcttctccat acgggcaatt caaatctcgg ctcggatttc caccatctcc
    16381 taaaagccca accgagagtc actggcacaa gcacccagca aatgagatag agcgggataa
    16441 gcacagacat tattcccatt tccgcacctc ccagaggcgg ttcaattcct cacttcactg
    16501 aaggatttgt cgaggagctt gatggcttca tcgacgtcgg gcttggtcgt attcagcatg
    16561 ggggcgaggc ggagggtgtt gccgtagagg ccgcctttgc cgatgagcag gccgttctgg
    16621 cgggcgcgct ccatcacttg cgtggcggct tcgggagcgg gctctttcgt cttgcggtct
    16681 ttcaccaatt cgagcccctg catcaatccc atgccgcgca cgtcgccgat gagcgaatac
    16741 ttcgcctgca actcttccag tttgccgcgg aagtacgtgc cgaccacgtc cgcgttctcc
    16801 agcaaattct cttcttcaat cagctcgatg gtggccttgg ccgctacgca tgtcacgggg
    16861 ttgccgccga atgtggcgat gttcgcaccc tggaacccgc cggcgatttc cggcgtggtc
    16921 acggtcaggc ccacgggcac gccattggcc atgcctttcg cgctggtgat catatccgga
    16981 atgacttccc actgctcgat gccaaaccat ttctttccgg tgcgtcccca gccggtttgc
    17041 acttcgtcgg cgatgaacag cgcgccatac tttttcacga ttttgaagac aagcttgaag
    17101 tactccttcg gcggagtgat aaatccaccg acgccttgaa tcggttcggc gatgaaggcg
    17161 gcgatgttgc cgctggttcc tgtttggatg aggttttcca cgtctcccgc gcaggccacc
    17221 tcgcaatccg gatatttcaa gtgcaacggg cagcggtagc agtagggatt cacggcatgc
    17281 gaaatgccca cgctgatcac gccgcccttg cggtacggcg cgtgcgcggt gacggctttg
    17341 gcgagcgccg agccgccgga ataggcgtgg cgcagggcca ccacgtcgta gctgccggtg
    17401 gacatgcgcg cgagcaggat ggcggcttcg ttggcttccg tgccggagtt ggtgaaaaaa
    17461 ctctgctgca ggttgcccgg cgtaatttgc gcgatctttt cagcgagcgc cacgatgtgt
    17521 tcgttgggat agagcgtcga agtgtgctgc agccgattga cctgcgcgct cactttgctg
    17581 gaaatcttcg ggttgcagtg acccacggag acggtcagga tgccgccgaa gaaatcgaga
    17641 tacttgttgc cttccacgtc ccataggtgt tgcatttcgc cgcggtcggc caccagcggc
    17701 tgctggaaat agttggtcac cgagggccac agatattttt tgtgcttggc gacgacttct
    17761 tcgctgcgcc cgctacgggc aagttgggat gcaggtacga aatgtgcggg tgtggtcacg
    17821 gataagtcct cgtgtgatcg ataggatgct ctgagtttac gacggggcgc tgaaggcggc
    17881 aagggagggc gaggaatggc ccatcaggct tcgagtgttc tgcggcatgg agctccttgg
    17941 ccgaccgctc gcggagttcg agcgaagcgt atagtgaagg aatggctcga tttgaagagg
    18001 acgcaaacgg tcgcgaggtg agtctccgcg taggcgaagt gtttgagata tgcctgccgg
    18061 agacgcggac aacgggctac aagtgggcgg tcgaaaaaag cggcgagccg gtttgcggct
    18121 tgacgggtga atcggcggat gcacctgccg gaccgcccgg acgagcgggg acgcacctct
    18181 ggcaatttcg cgccgcgcag gccggcacag cgacgatcct tctccatcat cggcgctcgt
    18241 gggaatcggg cgtagcgccg ggacgtattt ttcaaatcca tatccaagcg acagaatgag
    18301 agtggtgcag cgtgcgcgtt cctaaaccgc gtacgctgcg gatttgccac ggcgtctacg
    18361 cgaccaggcg aatggtccag aaatcgtcgg ccaagttgct gtcggtcagg taggaatagg
    18421 gaatcgtgaa gtagcccgcc atcccccatc cgggacccca ggaattcctg acgataaacc
    18481 agtggtgagc gtcctcgtag cccacggcga ggacggcgtg acccccgatc tgtttttctc
    18541 cagtgtgggg catgggggcg tggccggtct gggcgacctg tgggctttca aagctttcat
    18601 aaaccgaaaa gccaagtacg aagggatagc ccgaggccag gcagcccttc atctggttga
    18661 gaatctgcgg aactcgtcgg taggaagtgg cggtgttttt tgcggcatcg gcgtagcaag
    18721 gcggatcggg cggcttgtcg gcaaacttgg tgatgtcgta gggccaatcc gtttccgggc
    18781 acacgccggt tgtggccacg ctcttgacgc cgtcgcgtat ctgcgcacca ttgtcggaat
    18841 tgacggaacc ttcgatcacc cgctcgttgt aataaatgaa gagccgggaa ggaatccaat
    18901 tggcgatgtt ctgcttcatc tgctcgaatt ggaaagcgcc gccgatggcg ttgccggtac
    18961 aactgccgag ttggccttgg t
//
LOCUS       DASYVO010000539         5542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_281218, whole genome shotgun sequence.
ACCESSION   DASYVO010000539 DASYVO010000000
VERSION     DASYVO010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281218"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VGR03_18670"
     CDS             <1..314
                     /locus_tag="VGR03_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996365.1"
                     /translation="VSIREQPAGLAHTAQMINMALRMGMSQAAAGDASASFRDVMQMT
                     PDRAEVVLQAKPTTRQRAMGILRRIKYAACFPLAVAAYSYVRMRGFKGTYMYCLARKC
                     D"
     gene            336..1403
                     /locus_tag="VGR03_18675"
     CDS             336..1403
                     /locus_tag="VGR03_18675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1996366.1"
                     /translation="MARSFVSVLIDTYNHERFIEQAIVSVLEQEFPSADREIIVVDDG
                     STDRTPEIVKKFEPRVRLLRKENGGQASAFNAGIPECKGEIVAFLDGDDWWAPNKLTR
                     VVQAMATDPSVGIVGHGIVNVQRDGREQAEILREGFRFQANTIEGARLFRRRGAFLGT
                     SRMTIRADLLRRIGPVPEAIEIQADEYLFTLAAVLAGAQILPEALTYYRLHEANSFQL
                     ASHDLQKLHRKQKSLATLASSLSEQLENLGIDPQVRRTVIEYTQASADQLRLGIDGGW
                     PWETVKTEWKIYEILHPEARFSHRVFKFTILLAALVTPPRNFYSIQRTLAQSGLYRRA
                     RAHWLPVPEMEHIQKDWRAGS"
     gene            1385..2146
                     /locus_tag="VGR03_18680"
     CDS             1385..2146
                     /locus_tag="VGR03_18680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEV1996367.1"
                     /translation="MARGIMIKAAIHKTFRLFGLDIIQFPAQEQAYPPDFRNDEAEII
                     REVRPWTMTSAERIYALIQAVRYVSASDIAGDIVECGVWKGGSMAAIARTLLQLQDVK
                     RDLYLFDTFEGMPEPTTKDIDYSGKSAAEVLREDSGTRCAEAPLERVKEVLYGTGYPR
                     ERIHFVRGRVEETIPASAPDSISLLRLDTDWYASTKHELVHLFPRLAHAGVIIIDDYG
                     HWKGSREACDEYFTQNRIPILLNRIDYTGRIALKP"
     gene            2227..3441
                     /locus_tag="VGR03_18685"
     CDS             2227..3441
                     /locus_tag="VGR03_18685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1996368.1"
                     /translation="MRILMISGVPGKAEAGVAGIVYNLAKELGELGHSVKPLFFEDLL
                     PTQSWPNRFRTVEFAKRVAEYVRETGKEFDIVNIHAPFGFWYGAQRRRRGSQAGPPYV
                     MTMHGLEERRNYAMGREAEKGRADYFRWKNRVWQHLYHMPTYRWSFKTADQCIVTNRE
                     ALLFLQLHYNLPPDRVWLVPNGVGTEFFHVRSLSGGIATKLLFVGAWIDHKGIYYLAE
                     AFEKVLRVIPEARLTIAGCSEPEEKVRRCFATAAQAALEVWPFVARAEISSLYAEHEI
                     FVLPSLMEGMPLVLLEAMASGMPVVTTESSGMTDLVEDSHDGLFVIPGDTESLSAVIV
                     RLCRDPELRLRLGSAAQEKMKRYTWNQAARRTEMVFYRAMGAWPEASAGGPGNETAEP
                     NVLASANPQVAP"
     gene            3663..4148
                     /locus_tag="VGR03_18690"
     CDS             3663..4148
                     /locus_tag="VGR03_18690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1996369.1"
                     /translation="MEIGVAEGASAVALRTTMNTEGTLYLVDPFHLSRVRTLNFLRRA
                     ARRVVSANASPKTAWIEAFSHEAVVAWNQPIDFLLIDGDHREEAVEQDWNDWHPHVME
                     NGIIAFHDARLFPGGWTSPEYGPVRFVDRAFRNNPSSSWTILEEVDSLVFVRRREHGS
                     A"
     gene            4145..>5542
                     /locus_tag="VGR03_18695"
     CDS             4145..>5542
                     /locus_tag="VGR03_18695"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HEV1996370.1"
                     /translation="MITLGINYSQMHDSSACIVRDGELLFAVAEERISRLKHDAGFPK
                     NAIRACLDFAKVRAEQLDEVCFGWQTAGPVFRHDLKCYATGGMPLTYANGLNSTLHFL
                     SMCHQESGAKKFAQQFGPTKAKMRFVDHHLAHAISAYAYSGYEDAAVVIMDGRGAWEA
                     TTIWHGHNGRLDPVLMIPFPDSVGYFYSEFTQFLGFQRNSDEWKVMGLAPYGKPGVDL
                     SAFIDLKAAPYRVHTKQLVANGATPFAGMTALLGPARIPESEIDDRHKDIAYAVQDAC
                     EIAMMSVVRMAIEKTRCRNVCLAGGVALNSKANGKIAASGLVDKMFVQPAASDDGVAL
                     GAALAPYLDDNGKLPNKRMRHGYWGPSFEDEAIESALRTYKLRYTRLPDPAATAAELL
                     SHGKILGWFQGRMEFGPRALGSRSILADPRDPEMNAKVNNAVKFREWWRPFAPSFKKE
                     VAGEYLESATDSPFMI"
BASE COUNT         1203 a         1441 c         1762 g         1136 t
ORIGIN      
        1 ttgtatccat tcgcgagcaa ccggctggac tcgcacacac ggcgcagatg atcaacatgg
       61 cactgcgcat ggggatgtcg caagctgcag cgggagacgc gtcggcgagt tttcgcgacg
      121 tgatgcaaat gacgcccgat cgagcagagg tggttttgca ggcaaagccg acgacacggc
      181 aacgcgcaat ggggatattg cgacgaatca aatatgcggc atgtttccct ctggcggtgg
      241 cggcatattc gtacgtgcgt atgcgcggtt tcaaagggac gtatatgtat tgtctggctc
      301 ggaagtgcga ttgaagattt tcggggagcc gtaatttggc aagaagtttt gtcagcgtac
      361 tgattgacac gtacaaccac gagcgcttca tcgagcaggc gatcgtgagc gtgctggagc
      421 aggagtttcc gtcggcggat cgtgaaatca tcgttgtgga cgatggctcg acggatcgca
      481 cgccggaaat cgtgaagaaa ttcgagccgc gcgtgcggct gctgcgcaaa gagaatggtg
      541 ggcaggcctc ggcgttcaat gctggcattc cggaatgtaa gggcgagatc gtcgcatttc
      601 tggatggcga cgactggtgg gctcccaata agctgacacg cgtggtgcaa gctatggcca
      661 cggatccgtc cgtgggaatt gtgggccacg gcatcgtcaa cgttcagcgc gacgggcgcg
      721 agcaagcaga gattctccgc gaggggtttc gattccaggc caatacgatc gaaggggcgc
      781 gcttgtttcg ccggcgtggc gcttttctgg gaacgagccg catgacgatt cgcgccgacc
      841 tgctccggcg gatcggacca gttccagaag caatagaaat tcaagcggac gagtacttgt
      901 ttacactcgc tgccgtgtta gcgggagcgc agattctgcc ggaagcgctc acctactacc
      961 gccttcatga ggcgaacagt tttcagctcg cgagccatga cctgcaaaaa ctgcaccgca
     1021 agcaaaaatc acttgcgaca cttgcaagca gtctttcgga gcagcttgag aacctcggca
     1081 tcgacccgca ggtacgtcgc accgtcatcg agtatacgca agccagcgcg gaccagcttc
     1141 gacttgggat cgacggcggg tggccgtggg aaacagtaaa gaccgaatgg aagatctatg
     1201 agatattgca tccggaagcg aggttttctc atcgcgtctt caaattcaca atcctgctgg
     1261 cggcattagt taccccgccg aggaactttt acagcatcca gaggacgctg gcgcagagcg
     1321 gcctgtatcg gcgcgcgcgc gcacattggc ttccggttcc cgagatggag cacatccaaa
     1381 aagattggcg cgcgggatca tgatcaaggc ggcgattcat aaaaccttca ggttattcgg
     1441 cctcgacatt attcagttcc ccgcgcaaga acaggcttat cccccggact tccgcaatga
     1501 tgaggccgag atcatccgcg aagtccgccc ctggaccatg accagcgcag aaagaatcta
     1561 cgcgctcatt caagcggtgc ggtacgtgag cgcgagtgac attgcgggag acatcgttga
     1621 atgcggggtc tggaaggggg gaagcatggc cgcaattgcc aggacgctcc tccagttgca
     1681 agatgtgaag cgcgatttgt atctcttcga tacctttgaa ggcatgccgg agcccacgac
     1741 gaaagacatt gattactcag gcaagtcggc cgcggaggtt ttgcgagagg actccggcac
     1801 gaggtgcgcg gaagcaccgc tggagcgagt gaaagaagtc ctctatggga cgggttaccc
     1861 gagggaaaga atccatttcg ttcgaggtcg agtggaggaa accatacctg cttcggcgcc
     1921 agactcgatc tcgctgctgc ggcttgatac cgactggtat gcgtcgacca agcacgaact
     1981 ggtccacctt tttccgcgcc tggcgcacgc gggtgtgatc atcatcgacg actacggaca
     2041 ttggaaaggg tcgcgggaag cctgcgatga gtatttcacg cagaatcgca tccctattct
     2101 tctgaatcga atcgattaca ccggccggat tgcgttgaag ccgtgacggt agccctacag
     2161 cgcagcgcgg cgcaggacgc tgtgttaagt attgccggcg agttggcaga gcgaggcaac
     2221 gcgtagatgc gcattctgat gatatcggga gtgccgggga aggccgaggc cggggtcgcg
     2281 ggcattgtct acaacctggc gaaggaactg ggtgagctgg gacacagcgt gaagcctctg
     2341 ttttttgaag acctgctgcc cacgcaatcg tggccgaatc gctttcgcac cgtcgagttt
     2401 gcgaagagag tcgcggaata tgtgcgggag actggcaagg aattcgacat agtcaatatc
     2461 catgcgccgt tcggcttctg gtatggagcg caaaggcggc ggcgcggttc gcaggcgggt
     2521 cctccgtacg ttatgaccat gcacggtctc gaggagcgcc ggaattacgc gatgggcagg
     2581 gaggccgaaa aagggcgcgc ggactatttc cggtggaaga atcgcgtgtg gcagcacctc
     2641 tatcatatgc cgacctatcg atggtccttc aagaccgccg atcaatgcat cgtgacaaac
     2701 cgggaggctt tgctctttct tcagttgcat tacaatctgc ctccggaccg cgtgtggctc
     2761 gtgccgaacg gtgttggaac ggagttcttc cacgtgcgat ccctctcagg tggaattgcg
     2821 accaagcttc tatttgtcgg agcgtggatc gaccacaaag gaatttatta tctggcagag
     2881 gccttcgaaa aagttctccg agtcattccg gaggcccggt tgacaattgc gggctgctca
     2941 gagccggaag agaaggtgcg ccggtgcttt gcgacggccg ctcaggccgc gttggaagtg
     3001 tggccgtttg ttgcgcgcgc ggagatttcg agtttatatg ccgagcacga aattttcgtg
     3061 ctgccctcgc tgatggaagg tatgccactg gtgctgctgg aggccatggc ctccggaatg
     3121 ccggtggtga cgacggaatc aagcggtatg actgacttgg ttgaggattc acacgacggg
     3181 ctctttgtta ttcccggaga cacagagtcg ctttcggcgg tgatcgtcag gctttgccgc
     3241 gatcccgagc tgcggctgcg tctcggcagt gccgcgcagg aaaaaatgaa gcgctatacc
     3301 tggaaccaag ccgcccgccg gacggagatg gttttctacc gtgccatggg ggcctggccc
     3361 gaggcctctg cgggcgggcc ggggaacgaa acagctgagc caaatgtgct cgcttcggca
     3421 aatccccagg tcgcgccata aactaactct caagcgttcg aggaagagcc aggaacgaag
     3481 tgactacgcc aaggattcga tttgccgaga ttcactcaga actccaagag ccggagatgt
     3541 ggacgtctcg ggtgatgaaa cacccgttct ttgcgtgggc cggattgcga ccgccgattg
     3601 cacagcatac ggcgaccgaa caccatgcgc tggagaagta cgcccgagga tgcaggagtc
     3661 tggtggaaat cggcgttgcc gaaggtgcgt ctgcggtcgc gctgcggacc acgatgaata
     3721 cggaaggcac actgtatctt gtggatccgt tccatctttc aagagtgcgg acgctgaact
     3781 ttctgcggcg cgccgccaga cgggtggtct ccgcgaacgc gtcacctaaa actgcatgga
     3841 tcgaagcctt cagtcatgaa gcggttgtgg cctggaacca accaatcgac tttctattga
     3901 tcgatggaga tcacagagaa gaagctgtcg aacaggattg gaacgactgg cacccgcatg
     3961 tgatggaaaa tgggatcatt gctttccacg acgcacgtct tttcccagga ggatggactt
     4021 ccccggaata cggaccggtt cgttttgtgg atcgagcttt ccggaacaat ccttcgtctt
     4081 catggacgat actcgaggag gtagattcac tggtgttcgt tcgcaggagg gaacacggca
     4141 gcgcatgatc accctgggga ttaattattc gcagatgcat gattcgtcgg cgtgcatcgt
     4201 gcgcgacggc gagctgttgt ttgcggtggc ggaagagcgc atcagcaggc tgaagcacga
     4261 tgccggcttt ccgaaaaacg ccattcgagc gtgtctggac ttcgcgaagg taagagcgga
     4321 gcagttggac gaagtgtgtt ttggctggca gacggcgggg ccggtcttcc ggcacgattt
     4381 gaaatgctac gccaccgggg gaatgccgct gacttatgcg aacgggctga attcgacgct
     4441 gcactttctg agcatgtgtc atcaggagag cggagcgaaa aaattcgcgc agcaattcgg
     4501 gccaacgaaa gcgaaaatgc ggtttgtcga tcatcacctg gcgcacgcca tcagtgcgta
     4561 tgcgtattcg gggtatgaag atgccgcggt tgtcattatg gatggccgcg gcgcgtggga
     4621 agcgacgacg atctggcacg gacacaacgg caggctcgac ccggtgttga tgattccgtt
     4681 tccggattcg gtgggatatt tctacagcga atttacacag tttctcggct ttcagcgcaa
     4741 cagtgacgag tggaaagtga tggggctggc gccttacggg aagccgggag tggatttgtc
     4801 cgcctttatc gatttgaagg cggcgccgta tcgcgttcac acgaagcaac tggtcgccaa
     4861 cggcgcgacg ccgttcgcag gcatgacagc gctcctcggc ccggcccgca ttccggagag
     4921 cgaaatagac gaccggcaca aggacattgc ttacgccgtc caggacgctt gtgaaattgc
     4981 gatgatgagc gtggtgcgga tggctatcga gaaaacgcgg tgccggaacg tttgcctcgc
     5041 cggaggcgtg gcgctgaatt cgaaagccaa tggcaaaatt gccgcatccg gcctcgtgga
     5101 caaaatgttt gtgcagcccg cggcttcgga tgacggtgtg gcgctcggcg cggcgctcgc
     5161 tccctatctc gacgacaacg gaaaattgcc gaacaagcgg atgcggcacg ggtattgggg
     5221 gccaagcttt gaagacgagg cgatcgaaag cgcgctgcgc acgtataaat tgcgttacac
     5281 gcggctgcca gaccccgccg caaccgcggc tgaactgctt tcacacggga aaattctcgg
     5341 ctggtttcaa gggcgcatgg aatttggccc gcgcgcgctc ggaagccgct cgattctcgc
     5401 cgatccgcgc gatccggaaa tgaacgctaa ggtcaacaat gcggtgaaat tccgggagtg
     5461 gtggcggccg tttgcgcctt cgttcaagaa ggaagtggcg ggcgaatacc tggagagtgc
     5521 cacggattca ccgtttatga tt
//
LOCUS       DASYVO010000540         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_193867, whole genome shotgun sequence.
ACCESSION   DASYVO010000540 DASYVO010000000
VERSION     DASYVO010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193867"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="VGR03_18700"
     CDS             complement(<1..1208)
                     /locus_tag="VGR03_18700"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEV1996371.1"
                     /translation="MKERKPIFYDVEKVRWRRTRRAMEISGLLLTALLVYFFVTIAIS
                     VELPAELLPDTKPKYQTLKAKKKPVPAREGRHRRVANIGTVPASYDPLRAAFFVSWDA
                     NSLASLKKHYKDIDLLIPEQLHAVTPDGALTIVDYERGQTQVKASPAEAIALLKYDKL
                     HQWMKSLNPPIELPMMGVVNNYDGVNWRIQEMAQLLASPAARQNLVRDIVEYAVESHE
                     AGIVIDFEEVPDVSQANFRTFIGALAPALHSVGLKLMIALPARDDDYDYEFFGKQCDA
                     IVLMNYDQHWLTSPPGPIASQDWFVENLHQLFDVVPPQKIVVGIANYAYDWSAAPKKE
                     YEPAAEFSIQEALLHAKESESDVEFDSDSLNPHYSYSDEHNHVHQVWMLDAITAYNEL
                     QASDRLGVQGT"
     gene            1306..2781
                     /locus_tag="VGR03_18705"
     CDS             1306..2781
                     /locus_tag="VGR03_18705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996372.1"
                     /translation="MRRIFSNPITALLGGACLRLLFVLKFPSNSGDTVIYDQLAANWL
                     KFGKYAMNVNGQLIPVDLRMPGYPAFLALIYALTGRTGDNARLYVMLAQIVIDLATCA
                     LVASLAVLLASLWISPGRTRRIFVAGFWLAALCPFTANYVAVPLTEVWATFFTTASLA
                     VLLLIALKARGHRIPDIPPKIANWDYWKLVALGGFLVGMGTLFRPESPLLLITTCFLL
                     GCWLLRQGEFKRFVLTSALLGCACLLPLGPWIIRNARTFHEFQPLAPKDATLPTEIDP
                     RGFMAWEKTWLYRFKDCYLVPWKLNDEAIRLEDIPANAFDSEEEKQRVAEVLEKYNGE
                     TNWTADEDALFAQIGKERTARHPLRTYLWIPLRRSVRIWFTPRIELLPVSGNVFPLRS
                     MWEQDPVDQRVTILFFLLNIAYVCLALWGACRLWSYTNARAAVVLLLFYVVVRTAFLT
                     TLETPEPRYVLVCFPVLLAFAAQVMAGRQETPAPTPRSKAT"
BASE COUNT          534 a          871 c          860 g          699 t
ORIGIN      
        1 gtgccctgca cgcccaaccg gtcgcttgcc tgcagttcgt tgtaggccgt gatggcatcg
       61 agcatccata cctgatgcac gtgattgtgt tcgtcagaat acgaatagtg cgggttgagt
      121 gaatcgctat caaactcgac gtccgactct gattctttcg cgtgcaacag tgcttcctgg
      181 atactgaact cggctgccgg ttcgtattct ttctttggag ccgcggacca gtcgtaagcg
      241 tagttggcaa tccccacgac gattttttgc ggaggaacca cgtcgaacag ctggtggagg
      301 ttttcgacaa accaatcttg cgaggcgatg gggccaggcg gggaagttag ccagtgctgg
      361 tcgtaattca tcagcacgat ggcgtcgcac tgcttaccga agaattcgta atcgtagtcg
      421 tcgtcgcgcg cagggagagc gatcatcagc ttcaacccca cggagtgcag cgcaggcgcc
      481 agcgcgccga tgaatgtgcg gaagttcgcc tggctgacgt cggggacttc ctcaaaatcg
      541 atgacgatgc ccgcttcgtg cgattccacc gcgtattcca cgatgtcccg gaccagattc
      601 tggcgcgcgg cggggctcgc aagcagttga gccatttcct ggatgcgcca attcacaccg
      661 tcgtagttgt tcaccacacc catcatgggc agctcgatgg ggggatttaa cgacttcatc
      721 cactggtgca gcttgtcgta cttgagtagc gcgatggcct cggcaggcga ggctttcacc
      781 tgggtctggc cgcgttcgta gtccacgatg gtcagcgctc cgtccggcgt gactgcatgc
      841 agttgctcgg gaatcaggag gtcgatgtct ttgtaatgtt ttttcaggga agcgaggctg
      901 ttagcatccc agcttacgaa aaacgccgcg cgcaatggat cgtagctcgc gggcacggtt
      961 ccgatattcg ccacgcggcg atgccggccc tctcgcgcgg gtacgggctt ctttttcgcc
     1021 ttcagcgtct gatacttcgg tttggtatcc ggcagcagct cggcgggcag ctccacgctg
     1081 atggcgatag tcacaaaaaa ataaaccagc agcgcagtca gcagtaagcc ggaaatttcc
     1141 atcgcgcgcc gcgtgcggcg ccagcggacc ttttccacgt catagaatat gggcttgcgc
     1201 tctttcatgc cgcttggtcg ccccaccggc ccaagaacac cggggaagca ttcgtcactc
     1261 cgtcgaatcg aactgagctg ctctattgta agatgccaac aagcaatgag gcgaattttc
     1321 tccaatccta tcaccgcact tctgggcggg gcctgtttgc ggctcctctt tgtgctgaag
     1381 tttccgtcca attccgggga tacggtaatt tacgatcagc tggccgcgaa ttggctgaag
     1441 ttcggcaaat acgcgatgaa tgtgaatggc cagttgatac cggtggattt gcggatgcct
     1501 ggttatccgg ccttcctggc gctgatttac gctttgaccg ggcgaaccgg ggacaatgcg
     1561 cggttatacg tgatgctcgc gcagatcgtg attgatctgg cgacctgcgc gctcgtcgcc
     1621 tcgctcgcgg tgctgctggc ttcgctttgg atttcgcccg gtaggactcg gcgcattttt
     1681 gtcgcgggct tctggctggc ggctttgtgc cctttcactg cgaattatgt tgccgtgccg
     1741 cttaccgaag tctgggccac tttcttcacc accgcgtcgc tggcagttct cctgctgatt
     1801 gcgctcaagg cccgtggaca tcggattccc gatatcccgc cgaagatagc gaattgggac
     1861 tattggaagc tcgttgcgct cggcggattt cttgtcggga tgggaacgct gttccggcca
     1921 gagagtccgc tgctgctgat caccacctgt ttcttgctcg gctgctggct tctccgccag
     1981 ggcgaattca agcgttttgt gctcaccagc gctttgttgg gatgcgcctg ccttctgccg
     2041 ctgggtcctt ggataatccg caacgcccgc acattccacg aatttcagcc gctggcgccg
     2101 aaggatgcca ccttgcccac agaaatcgat ccccggggat tcatggcctg ggagaaaacc
     2161 tggctctatc gcttcaaaga ttgctaccta gtgccgtgga aattgaacga tgaagcaatc
     2221 cgcctcgaag acatcccggc gaatgccttc gactccgagg aagagaagca gcgcgtcgcg
     2281 gaggtgctgg aaaaatacaa cggcgagact aactggactg cggatgaaga tgccctcttc
     2341 gctcagattg ggaaagagcg aaccgcgcgc cacccgctcc gcacctatct ctggattccc
     2401 ctccggcgca gcgtgcgcat ctggtttacc ccgcgcatcg aattactgcc ggtttccgga
     2461 aatgtcttcc cgctgcgctc catgtgggag caagaccccg tggatcagcg cgtcaccatc
     2521 ctttttttcc tcctgaatat tgcttacgtg tgtttggcgc tttggggcgc gtgccgcttg
     2581 tggagctaca ccaacgctcg cgcggcggtt gtactacttc tcttctacgt cgtcgtgcgc
     2641 accgcgttcc tgaccaccct ggagacgcct gagccccgct acgtgctggt ttgctttccc
     2701 gtgctgctcg cgtttgccgc ccaggtgatg gcaggaaggc aggaaactcc agcacccacc
     2761 cccagatcga aggctactta gagtcatttt acctctcaga tcgcgcaccg cccgccgtac
     2821 tcgcccacgg gaagggttcg tgctactctt ttccgctgct tccacgccca tcgcaacggg
     2881 taggtcatga atgattcagt gagtacgggc cgcgcggcca cgcgtgtggg agccatcctg
     2941 ttcgcggcgg tagtcctggc gtgg
//
LOCUS       DASYVO010000541         6601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_194124, whole genome shotgun sequence.
ACCESSION   DASYVO010000541 DASYVO010000000
VERSION     DASYVO010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194124"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VGR03_18710"
     CDS             complement(<1..744)
                     /locus_tag="VGR03_18710"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV1996373.1"
                     /translation="MFCSKCGAQISPNGRFCSSCGQAVDPDTGATLLGDEASLEGATI
                     APASLAPRKTPSSRPSPRTPRTLASSNPLSSSDLIGGGRFTPGQIIARRYRVVALAGR
                     GGMGEVYRAEDLTLGQVVALKFLPETLSQDATALARFHAEVRTARQVSHPNVCRMFDI
                     GDADGTLFLTMEYVDGEDLASVVRRIGRLSPDKATEIARQICAELAAAHERGVIHRDL
                     KPANVMLDGAGKIRLTDFGLAGIAASIKGA"
     gene            complement(802..1143)
                     /locus_tag="VGR03_18715"
     CDS             complement(802..1143)
                     /locus_tag="VGR03_18715"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEV1996374.1"
                     /translation="MPRVDFGMVTSALELPGYRIVRNFGIVRGIIVRSRSVIGNLGAA
                     LQTIVGGNITILTNLCEKTREDAFELLLQHAAEHGANAVVAMRYDATEVMQGVTEVLA
                     YGTAVQVERIS"
     gene            1237..2430
                     /locus_tag="VGR03_18720"
     CDS             1237..2430
                     /locus_tag="VGR03_18720"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1996375.1"
                     /translation="METLFYFLAFLQILLGVYLIWQVLQWLGYVRRHLHTDPGFYAPR
                     VAVLCPCKGIEPGLERNLLALTEFDRQNYEIFFILASGSDPARSIVERVAKSSRVKAH
                     VIIAGSPSTSGEKVNNLRVAVEQLPPEFEVLVFTDSDGRPGKGWLHHLVAPLGDVRIG
                     ATTTMRWLIPNRSNLPTALLAAWNAPVVTMLSEKGRNFCWGGGTAIRRSIFEQSGVMD
                     EWRNSVSDDYSLTRALERNNRSIVFIPECLTLSYVETDFPGLLEFTNRQILITRVYAD
                     KVWAPAAATHLLYCMTLLLGVILILSDIIEQRPAFHIATLTFLPVLLSSIRSSIRLIG
                     ATESLPAGRSQIVGQAWIYILLTVFVPFLYVVNFVNSLVTRKIRWRSMAYELISPEQT
                     RIIQF"
     gene            2882..3502
                     /locus_tag="VGR03_18725"
     CDS             2882..3502
                     /locus_tag="VGR03_18725"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1996376.1"
                     /translation="MKVIRRSEHPQQLTNSRPAFPRDVFFFYEKSTGVAHFHVDAGPE
                     GRFPVDQAAGLLAMHCMVRGQSTSDYVVMVQAGDGVLEGLNEKADKLLEAGKSVRSQV
                     KLTRREEEVLSGLMKSLANKEIANSLNLSERTVKFHVSSLLAKFRVRGRMELVREASR
                     QTACGMPMPMPVATREARTFAPVARNYPAARRSVAVVPLAKRQLMA"
     gene            complement(3593..4210)
                     /locus_tag="VGR03_18730"
     CDS             complement(3593..4210)
                     /locus_tag="VGR03_18730"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV1996377.1"
                     /translation="MAFTVPPLPYSFDALEPYIDAKTMEIHHDKHHGAYVTNLNKALE
                     GQAGLQAKSLDDLLKGLDAVPENIRNAVRNNGGGHWNHSLFWTLMKKGGGGEPKGNLA
                     AAIISTFGSLAGFQEKLAAAGLGRFGSGWAWLVVRDGKLAIDSTPNQDTPYLLAGGSK
                     AVLGLDVWEHAYYLKYQNVRGDYIKAWWNVVNWDKAAELYDAAKK"
     gene            complement(4515..5651)
                     /gene="queG"
                     /locus_tag="VGR03_18735"
     CDS             complement(4515..5651)
                     /gene="queG"
                     /locus_tag="VGR03_18735"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEV1996378.1"
                     /translation="MMPFADTLWIVDHAKSVGFDLCGVVRAEKFPELEQTSEWLARGY
                     AGEMKYLADPRRSDPQSAMPGIRSAIVGLLNYNTAQPLSTDPALPIDDGKPSGWISRY
                     AWGDDYHDVLRERLDALVESLRDRFTSPFVARTYVDTGPVQERVLAKYAGLGWLGKNT
                     LLLNQMLGSFFFLGVILTTLDLEPTLGANELPPPDLCGSCRRCLDACPTQAFVEPYVM
                     DARKCISYLTIELRGSIPEEFREPMGNHVFGCDICQDVCPWNRRAPIATIPQFQPRVF
                     PSPEENRTGASLPPQDESLYLPRLEWLIGLSEADFRELFRGSPVKRTKWRGLLRNACI
                     ALGNSSLRPATTDHDRIIDILERLAASEDQVLAESARWALLRIQ"
     gene            complement(5736..6080)
                     /locus_tag="VGR03_18740"
     CDS             complement(5736..6080)
                     /locus_tag="VGR03_18740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV1996379.1"
                     /translation="MSLHATHRDAGQVTVVDLSGRIVLGDGSALLRKTIRGLLDDQRK
                     MILLNLADVDYIDSSGIGELVSGFTGVKNQGGDLKLLHLTKKVRDLLQITKLYTVFDV
                     FTDESTALRSFS"
     gene            complement(6089..>6601)
                     /locus_tag="VGR03_18745"
     CDS             complement(6089..>6601)
                     /locus_tag="VGR03_18745"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1996380.1"
                     /translation="APGAAATQKMASDTKHLEVTLETQVESVNLAEEMCLRVAEAAGF
                     GEDDCYRIGMSVREGVINAYHYGNKERPDKKIHLAVDLTADKMIIHVLDEGPGFKLSD
                     VPDPLAEENLLSTSGRGIFLMRAFMDEFDVVSGRTGGAEIVMSKKLPAPGSASPNGQG
                     GQNVTPQQEA"
BASE COUNT         1356 a         1911 c         1900 g         1434 t
ORIGIN      
        1 cgcgcccttg atactcgcgg cgatgccggc aaggccaaaa tccgtgaggc gaatcttgcc
       61 ggctccgtcg agcattacgt ttgccggttt caggtcgcgg tgtatcacac cgcgttcgtg
      121 ggctgccgca agctccgcgc agatttggcg cgcgatctct gtggccttgt ccggcgaaag
      181 cctgccgatg cggcggacga ccgaagcgag atcctctcca tcgacgtact ccatcgtcag
      241 gaaaagagtt ccatcggcat cgccgatgtc aaacatgcgg cagacgttgg gatgtgagac
      301 ttggcgggcg gtgcgcactt cggcgtgaaa cctcgcgagc gctgtggcat cctgggaaag
      361 cgtctcgggg agaaatttca acgcgacgac ctggccgagc gtcaaatctt ctgcgcgata
      421 gacctcaccc attccaccgc ggcctgccag ggccacgaca cggtagcgtc gcgcgatgat
      481 ctgccccggt gtaaagcggc cgccgccgat gaggtcggaa ctcgacaggg gattgctgct
      541 cgctagtgtg cgtggagttc ttggggaagg acgtgatgac ggagtttttc gcggggcaag
      601 ggacgccggt gcgatcgtcg ctccctcgag gctggcttca tccccaagca gagtagcgcc
      661 cgtgtccgga tcgactgctt gtccacacga ggagcagaac ctgccgttcg gggaaatctg
      721 tgcaccgcat ttcgagcaga acatgggagg cggggattct accactggtc cagggcccgc
      781 cgcgaacgcc tgcgggagca gctaggaaat gcgctcaacc tggacggctg tgccataggc
      841 aagcacttcg gtaacccctt gcatcacttc ggtcgcatcg tagcgcatgg cgaccaccgc
      901 gtttgctcca tgttccgctg cgtgctgcag taacagttca aaggcgtcct cgcgcgtctt
      961 ctcgcagagg ttcgtgagaa tggtgatgtt tccgccgaca atggtctgaa gtgccgcgcc
     1021 gagatttccg atcacgctcc gcgagcgcac gataattccg cgcacgatgc caaaatttct
     1081 gacgatccga tagccgggca gttcgagggc tgaagtcacc attccaaaat ccactcgtgg
     1141 catttttgtt ctccttggaa caggcgttta ccggtgcgat tctaaccgtt cagacggcgc
     1201 tctgcgagca atgttcttct gctattctca gaaagcgtgg agacactatt ttattttctg
     1261 gcgtttctgc agatcctcct tggggtgtac ctcatctggc aggttttgca gtggctcggc
     1321 tacgtgcggc ggcacctgca cacggatccg ggattctacg caccccgtgt ggccgtgctt
     1381 tgcccctgca aaggcatcga gccgggcctg gagcgcaatc ttttggcgct gacggaattc
     1441 gaccgccaga actacgagat ctttttcatt ctcgcctcag gctcagatcc ggcacggagc
     1501 atcgtcgagc gggtagctaa gagttcaaga gtaaaagccc acgtcatcat cgcagggagc
     1561 ccgagcacgt ccggcgagaa ggtgaacaat ttgcgggtgg ccgtggaaca attgccgccg
     1621 gaatttgaag tacttgtctt cactgattcc gacgggcgtc caggcaaagg ctggctgcat
     1681 cacctggtgg cgccgctggg agatgtaaga atcggcgcga ccaccaccat gcgttggctg
     1741 attcccaatc gcagcaatct gccgaccgcg ctgctcgccg catggaatgc tccggtggtc
     1801 accatgctca gcgagaaagg tagaaatttc tgctggggcg gtggcaccgc cattcgccgg
     1861 agcatcttcg agcaatcggg tgtgatggac gaatggagaa actcggtcag cgacgattat
     1921 tccttgacgc gtgcgctgga gcgcaacaat cgctcgattg ttttcatacc ggagtgcttg
     1981 acgctttctt acgtcgaaac cgatttccca ggactgctgg agttcacaaa ccgccaaatc
     2041 ctgatcacgc gcgtgtacgc ggataaagtg tgggcgcccg cagccgccac gcatcttctg
     2101 tactgcatga cactcttgct cggcgtgatt ctcatcctga gcgacattat cgagcagaga
     2161 cccgcgtttc acatcgcgac gcttacattt cttcctgttc tactttcctc catccggagt
     2221 tccatccggc tcatcggcgc aacggagagc ctccctgccg ggcgatccca gatcgtggga
     2281 caagcctgga tttacattct gttaacagtt ttcgtcccgt tcctctacgt tgtcaatttt
     2341 gtgaactctc tcgtcacacg caagattcgc tggcgcagta tggcatacga attgattagc
     2401 ccggagcaaa cgcgaatcat ccaattctga agctctcgat tgcgtgaggc tgggcctcgg
     2461 cgttttccac aggcatagag agagtccctg ctctttcgaa ttcttcctcc tatcctaacc
     2521 ctttccccgt aagatggaaa aatttcgtgc accgtgcaat tcctaccgtc aaatgagaaa
     2581 gaaaacgtcc aaactctggc gtaagtcctc aagttttgag taagttgcat attcttccat
     2641 tcggaagaat atttgcaggg agggagggtt cggcaaaacc gtctcaggcg gactttcagg
     2701 gagttcctag acgcctggtc gtcgccgatc agtgggtaag agtcgccgaa ccatcagccc
     2761 tctttttggc ttcttgcctg actgtctacc catctgggga ttacgcctct tcgaggggcg
     2821 aaaagcataa aactcaatag gcttcggttc cgtcacgagg ctgcattcgg agggggttca
     2881 cgtgaaggtc atccgcagaa gtgaacatcc gcagcaattg acaaattccc gcccggcttt
     2941 tccccgcgat gtgttctttt tctatgaaaa aagcactgga gtggcccatt ttcatgtcga
     3001 cgccggaccg gagggcaggt ttcccgtgga ccaggccgcc gggctgctgg cgatgcactg
     3061 catggtgcgg ggccagtcca caagcgacta cgttgtgatg gtccaagccg gagacggagt
     3121 cctggaaggc ctcaacgaga aagcggacaa gctattggag gcggggaagt cggtccgcag
     3181 ccaagtgaag ttgacgcggc gggaagaaga agttctctcc ggcctgatga agagcctcgc
     3241 caacaaggaa atcgccaaca gcctgaatct ttccgagcgc acggtaaaat tccatgtctc
     3301 atcgctgctc gcgaaattcc gtgtgcgcgg ccgtatggag ctggttcgcg aagcttcgcg
     3361 gcaaaccgcc tgcggcatgc ccatgcccat gccggtggcg acacgggaag cacgcacttt
     3421 tgcgcccgtg gcaagaaatt atcccgccgc gcgccgttca gtagccgtcg ttccattggc
     3481 caaacggcaa ttgatggcct aattcggatt ccatctcagt tcttgagata aaagaaaagg
     3541 cccccgatcg gttcgggggc cttctctttt cgagaaaact taaaggaaaa gactacttct
     3601 tcgctgcatc gtagagttcg gcggctttgt cccaattgac gacgttccac caagccttga
     3661 tgtaatcacc gcgcacgttt tgatacttca ggtagtacgc gtgttcccag acgtccagcc
     3721 caaggacggc cttgctgccc cccgcgagca gatagggagt gtcctgattc ggcgtggagt
     3781 cgatggccag cttcccatct cggacgacca gccacgccca gccggaacca aagcggccca
     3841 ggccggcggc tgcaagtttt tcctgaaatc cagccagcga gccaaacgtc gagatgatcg
     3901 cagccgctag attccccttg ggctctccgc caccgccctt tttcatcaac gtccaaaaga
     3961 gggagtggtt ccagtggccg ccaccattgt tgcggacggc gttgcgaata ttttccggga
     4021 ccgcatcgag acctttgagg agatcatcga gcgacttggc ttgcagtccg gcttggcctt
     4081 ccagggcctt gttgaggttg gtgacgtagg caccatgatg tttgtcatga tggatctcca
     4141 tcgtcttggc gtcgatatat ggctcgagtg cgtcaaacga atacggcaac gggggcacgg
     4201 taaaggccac gggtttttcc tccagagtaa gatttgaatc caaattgttc gcggtgccgg
     4261 cgcgcaaaag tccaggtgca gcatttcctg ccccaagcaa tcctgcgccg acgatacccg
     4321 tattcaataa aaagttacga cgattcatag gaacctcagc agcgcggagg gcgaatcgac
     4381 ctgcgggtgg gattataccg ctactgcccg cgggatggta actcggccta agaaaaccgc
     4441 gattccgccg atctcatcgg tttgccagat gggcttatca cggtctgtcc ctccagcgac
     4501 tgttccttgc gctctcattg gatgcgcaaa agggcccaac gggcagattc ggcgaggacc
     4561 tgatcctccg aagccgcgag ccgctctaat atatcgatga ttcgatcgtg atccgtcgtg
     4621 gccggccgta ggctggaatt tccgagcgcg atgcaggcgt tgcgcagcag accacgccat
     4681 ttggtacgtt tgacggggct tccgcgaaag agttcgcgaa agtcagcttc gctcagcccg
     4741 atgagccact ccaatcttgg aaggtagagt gattcgtctt gcggcgggag agacgcgccg
     4801 gtgcggtttt cttctggtga aggaaaaact ctcggctgga actgcggtat cgtggcaatc
     4861 ggtgcgcgcc ggttccacgg gcaaacgtcc tggcagatgt cgcagccgaa aacatgattt
     4921 cccatcggct cgcggaactc ttccgggatg gagccccgca gttcgatggt caggtaggaa
     4981 atgcactttc gggcatccat gacgtagggc tcgacgaaag cctgcgtcgg gcaggcgtcc
     5041 aggcaacgcc ggcagcttcc gcacaaatcc ggcggcggca attcgttcgc cccgagcgtc
     5101 ggttcgagat cgagagtggt gagaatgacg ccgagaaaaa agaaggagcc gagcatctgg
     5161 ttgagcagca gcgtgttttt cccgagccat cccaaaccgg catatttcgc aagcacgcgc
     5221 tcctgcacgg gccccgtgtc cacataagtg cgcgctacga acgggcttgt aaagcggtcg
     5281 cgcagggatt ccacgagcgc gtccagcctc tcccgcaaca catcgtgata atcgtcgccc
     5341 caggcataac gagaaatcca cccactcggc ttgccgtcat cgatcggcag cgcaggatcg
     5401 gtggaaagcg gctgcgcggt gttgtagttg agcaagccga cgatagcgct ccgaattcca
     5461 ggcatggcgg attggggatc ggatcgccgg ggatccgcga ggtacttcat ttcgccggca
     5521 tagccccggg cgagccactc cgaagtttgt tcgagttccg ggaatttttc cgcgcggact
     5581 acgccgcaga ggtcgaaacc gacggatttc gcgtggtcaa caatccacaa cgtatcggcg
     5641 aacggcatca tccaggagat tgtgccgcag tcgagcatat caagcgagct gaaggacgaa
     5701 tccgatgctg cactcgaata ggccgccggg tgagatcaac taaaacttcg aagcgcggtg
     5761 ctctcgtctg taaatacatc aaatacggtg tagagcttgg tgatctgaag aagatcgcgg
     5821 actttcttcg tcaagtgcag caacttcagg tccccgccct gattcttcac ccccgtgaac
     5881 ccgctgacca gttcgccgat tcccgagctg tcgatgtagt ccacgtcagc cagattcagc
     5941 agaatcattt tcctctgatc gtccagcagt ccgcggatcg tctttcgcaa caaagcactg
     6001 ccgtcgccta agacgatgcg gccgctcaaa tccacaacgg tgacttgccc ggcatcgcga
     6061 tgcgtcgcat gtagtgacat ctctgtcctt acgcttcctg ttgtggcgtg acgttttgtc
     6121 cgccttggcc attcggggaa gcgctccccg gggcgggcag tttctttgac atcacgattt
     6181 ccgcgccgcc cgttctgccg gaaaccacat cgaactcgtc catgaaagcg cgcatcagga
     6241 aaatcccgcg accggaagta ctgagcagat tttcttcggc gagcgggtcc ggaacatcag
     6301 aaagcttgaa acccgggccc tcgtccagca cgtgaataat catcttgtcc gcggtgaggt
     6361 ccacggcaag gtgaattttc ttgtcggggc gttccttgtt cccatagtga taggcgttga
     6421 tcacgccctc gcgcacactc atcccgatgc ggtagcagtc gtcttcgcca aaccccgccg
     6481 cttccgccac gcggagacac atctcctcgg cgagattgac gctctcgact tgcgtctcca
     6541 gcgtcacttc gaggtgcttg gtgtcggagg ccatcttttg cgttgctgct gccccgggag
     6601 c
//
LOCUS       DASYVO010000542         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_12903, whole genome shotgun sequence.
ACCESSION   DASYVO010000542 DASYVO010000000
VERSION     DASYVO010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12903"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1012)
                     /gene="lepB"
                     /locus_tag="VGR03_18750"
     CDS             complement(125..1012)
                     /gene="lepB"
                     /locus_tag="VGR03_18750"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV1996381.1"
                     /translation="MSPNDPNIDPNIDPNSGPNIEPSIDGEASHVAPADAPAPAVATA
                     APLPRGETAWETLRSLLVVLVGVLCFRTFIAEATVIPTGSMEKTIMVGDHLFLNKLLY
                     GPQLPYTSFRIPSMRAVRRQEIVAFHYPRNPATMYVKRVIGVGGDVVRMVNKKVSVNG
                     AALVEPYVQHLPGSMPLGDNFPPPIAQIETFPAWLGLDPGWAREMPNYIKDDGLHVPQ
                     GYLFVMGDNRDNSADSRFWGFVPEEYVVGEPLFVFWSYDAPTRDWMDEGLKGRLQFDA
                     SILWNFIKKTRWSRTGKFF"
     gene            complement(1018..>2340)
                     /gene="purF"
                     /locus_tag="VGR03_18755"
     CDS             complement(1018..>2340)
                     /gene="purF"
                     /locus_tag="VGR03_18755"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV1996382.1"
                     /translation="TYLGLYALQHRGQESAGIAAFDGRNIVSHKAMGHVEEVFSQEIL
                     NALRGHSAIGHTRYSTAGDTDLKNAQPITVSCQKGQVALAHNGNLVNAGSLRKELESR
                     GDIFQTTSDSEVVLHFLARSKQSGIPESISDALDRVVGAYSLVMLFRDSVFAIRDPRG
                     FRPLSIGRLDGAYVIASETCAFDLINATHIRDVEPGEMVILDGRGITSLSFTPPVQPA
                     QCIFEHVYFSRPDSVVFGRPVQTSRDMLGRQLAREHPVAADVVIPVPDSGVAAAIGYA
                     AESGIPLKFGLIRNHYVGRTFIEPSQAIRDFGVKMKLNPVRSLIEGKRVVLVDDSIIR
                     GTTSRKIVRIVREAGASEVHMRISCPPTVSPCYYGIDTPTKKELIAATHNIDEIREFI
                     GADTIGYLSLEGLRQAVGDTDNRFCLGCYTTHYPTTVQESLISMRSRE"
BASE COUNT          516 a          667 c          675 g          482 t
ORIGIN      
        1 cacaggttat cggtgcgctt cattataaat caacccaaag ttttatgttc ttgaggaact
       61 gggaaattgg tgcggcttgg ggagataggg gaatactttc cagggagagt gccgtgcgcg
      121 aagatcagaa gaactttccg gttcttgacc agcgcgtctt tttgatgaag ttccagagga
      181 tggaagcgtc aaattgcagg cgacccttca gaccctcgtc catccaatct ctggtcgggg
      241 catcataaga ccaaaataca aacaacggtt cacccactac gtactcttca ggtacgaagc
      301 cccaaaagcg gctgtctgcg gagttgtccc ggttgtctcc catcacgaaa agataaccct
      361 gaggaacgtg caggccgtca tccttaatat agttgggcat ttcgcgcgcc cagccggggt
      421 caagtcccag ccacgcggga aacgtttcga tctgggcgat gggcggcgga aaattatcgc
      481 ccaaaggcat gctgcccggt agatgttgga catagggctc aaccagcgcc gcgccattaa
      541 ccgaaacctt tttgttgacc atccgcacca catcaccgcc aacgccgata acgcgcttca
      601 cgtacatggt ggcgggattg cgcggataat gaaacgcgac aatttcctgg cggcgaaccg
      661 cgcgcattga cgggatgcgg aacgacgtgt aaggcaactg cgggccatag agtaatttgt
      721 tcaggaagag gtggtcgccg accatgatcg tcttttccat ggagccggtc ggaatcactg
      781 tggcttcggc gatgaaggtg cggaagcaga gcacgcctac caggacgacg agcaacgaac
      841 gcagcgtttc ccacgccgtt tcgccgcgcg gcaatggcgc ggccgtcgct accgcaggtg
      901 cgggtgcatc tgctggggct acgtgcgaag cttccccgtc tatactgggt tcaatattag
      961 gaccactgtt cgggtcgata tttggatcga tgtttgggtc attaggcgac ataaaattta
     1021 ttcccggctg cgcatcgaga tgagcgattc ttgcacggta gtgggataat gtgtcgtgta
     1081 acaaccgagg caaaaacggt tgtccgtgtc accgaccgcc tggcgcagac cttcgagcga
     1141 caggtaacct atggtgtcgg cgccgatgaa ctcacggatt tcatcaatgt tgtgcgtcgc
     1201 agcgatgagc tcctttttgg tcggcgtatc aattccatag tagcagggag aaaccgtcgg
     1261 aggacaactg attcgcatat ggacttcact cgcccccgcc tcccgtacga tgcgcacaat
     1321 cttgcggctg gttgtaccgc gaataatcga gtcatccacc agcacaaccc gcttgccttc
     1381 gatcaagctg cgcaccggat tcagcttcat cttcacgccg aaatctcgaa tcgcctgcga
     1441 gggctcgata aaagtgcggc ctacgtaatg gttgcggatc aaaccaaatt ttaacgggat
     1501 gccggattcg gccgcgtagc caatggccgc tgcgacgccc gagtcaggca cggggatcac
     1561 cacgtcagcc gccacgggat gttcgcgcgc cagctggcgg cccagcatgt cacgactggt
     1621 ttgcaccggg cggccaaaca cgacgctatc cggccgcgaa aagtatacgt gctcgaaaat
     1681 acactgcgcg ggctggacgg ggggggtaaa actgagcgag gttatcccgc gcccatcgag
     1741 aatcaccatc tcaccgggct cgacgtcgcg gatgtgagtg gcattgatca agtcgaaggc
     1801 gcacgtttcc gaggcaatca cgtaagcgcc gtccagccgc ccgatgctca aggggcggaa
     1861 cccgcgcgga tcacgaatgg cgaacacgct gtccctaaag agcatcacca gcgaatacgc
     1921 tcccaccacg cggtctagcg cgtcggatat tgattcggga atgccggact gcttcgagcg
     1981 agctagaaaa tgcaggacaa cttcgctgtc actcgtggtc tgaaaaatat caccacgact
     2041 ctccagctcc ttgcgcagcg acccggcgtt caccaggttt ccattatgcg ccagagccac
     2101 ctggcccttc tggcagctca ccgtaatggg ctgggcgttt ttcaggtcgg tatcgccggc
     2161 ggtcgagtag cgcgtgtggc cgatggcgga gtgcccacgc agcgcgttca aaatttcctg
     2221 gctaaatact tcctccacgt gccccatggc tttgtggctc acgatatttc gcccgtcgaa
     2281 ggccgcgatg ccggcgctct cctgcccgcg atgctgcagc gcgtagaggc ccagataagt
//
LOCUS       DASYVO010000543         3659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_238323, whole genome shotgun sequence.
ACCESSION   DASYVO010000543 DASYVO010000000
VERSION     DASYVO010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238323"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..2821)
                     /gene="glnD"
                     /locus_tag="VGR03_18760"
     CDS             complement(173..2821)
                     /gene="glnD"
                     /locus_tag="VGR03_18760"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEV1996383.1"
                     /translation="MTTAARPGFDPAIPAAKNMQGSPTPLSVLGPRADAERQRIRQQF
                     EAGTSARETLRALCELADLNIQQIFDDLLRVHNREPQGLCLLALGGYGRRMLFPYSDL
                     DILFLFGSERAEQESRPLISEFSRTLWDLGFRVSSAGRTLEECKRIEEDNAEFHLALL
                     DRKFLAGDSGLFEKLDTKVLPASEKQARPFLLAQLHRLTRERLTRYGNTIFHLEANVK
                     ESPGGMRDYQATVWLRQILDEKKEIRKITLTEEELSAAAVDFLSAIRCFLHYSNGRND
                     NTLTYELQAAAAERALGVGDGISRNAAEWMRLYFRHARTLNRQLLRYLEQKSPVPLTL
                     RQRLLNAARASRVEPDHGNYFMVRDGLLEVVDQPAFSDRAVTYSLFAEAARTGTPLSR
                     EAERSIGYILTHPELPPRNTEMPWSTLHEILGSDYPGVALRPMQRLGLLTQILPEFGM
                     IDSLVVRDFYHRYTVDEHSLRTIEHLQELADPPDTRGTHFVPLWKTVERRDLLILSLL
                     LHDVGKGMPAENHITGSLAALDSAARRLKLSPEEKAEVQFLIEHHLDMSATVQRRDIF
                     DPATVSAFAQSVVTQERMQRLCLLTYADIHSVNPEALTPWKAEMLWQLFVATSNHFSR
                     TLDRDRLHALDEVSLLERVKALASVAGTEEIERFLEGFPRRYLAVHSAAEIAAHFALY
                     QKLSADPVQTELIATRHAFSLTLLAADRPALFATIAGVLAGWGMNIIKADAFANAAGV
                     VLDTFHFADLHRTLELNPSEIERFRKSLAHVVMGKAPLEPLLKSRESASRARTPKVAV
                     ETRINFDDTSSAHSTLMEIVTQDHPGLLYEIGSVLARLGCNIEVALIDTEGQKAIDVF
                     YLTAQGKKLTAQKQELLREVLQGTLV"
     gene            complement(2878..3216)
                     /locus_tag="VGR03_18765"
     CDS             complement(2878..3216)
                     /locus_tag="VGR03_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEV1996384.1"
                     /translation="MMKIEAVIQPSRFESVKDALREIGIEGMTVTEVRGHGRQKGHTE
                     VYRGREYTVDLLPKIKLEMVLPDALVNRAVDTILKAAKTGKIGDGKIFLSKVEEAIRI
                     RNEERGENAL"
     gene            complement(3265..>3659)
                     /locus_tag="VGR03_18770"
     CDS             complement(3265..>3659)
                     /locus_tag="VGR03_18770"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996385.1"
                     /translation="ALVIGLIAGVLCYLMVTKVKAIFGYDDALDAFGVHGAGGTIGAL
                     LTGVFAQQVINPIFGAGKAVGGLDGHWGQVGNQLVGILIAWGLALVGTIVLLKIVDLL
                     TGVRVPEEHEIEGLDITQHGEEAYNLES"
BASE COUNT          723 a         1125 c         1028 g          783 t
ORIGIN      
        1 ctcggtagcg aacttctcaa aagcagtccg ggtgtcacgg agttgacatg agacaacaaa
       61 ttcgcaggtt caaagcgacc gcaaatcgtc caagtttggc ctccacaccg cattcgagtt
      121 cttcagctac ttcatctttt accggagtga gattcgacta acggagccgg ggttaaacca
      181 gagtcccctg aagtacttcg cgaaggagtt cctgtttctg tgcggtgagc tttttgccct
      241 gcgccgtaag ataaaaaacg tcgatggcct tctggccttc cgtatcgatc agcgcgacct
      301 cgatgttaca tcccaagcgc gccagcaccg agccaatctc atacagcagg ccgggatggt
      361 cttgcgttac aatttccatc aaagtactgt gcgcggagga cgtatcgtcg aagttgatcc
      421 gcgtttccac ggcaaccttc ggcgttcgtg cgcggcttgc gctttcgcgg ctctttaaga
      481 gtggctcgag cggcgccttc cccatcacga catgcgcgag gctcttgcgg aagcgctcta
      541 tttcgcttgg attcagctcc agcgtgcgat gcaagtctgc gaagtggaag gtgtccagta
      601 ccacgcccgc ggcattcgcg aaggcatccg ccttgatgat gttcattccc cagccggcca
      661 gcacaccggc aatggtggca aagagggccg gacggtccgc cgcgagcagc gtcagcgaga
      721 acgcgtggcg cgtggcgatc aattcggttt gcaccggatc cgcactcaac ttctggtaca
      781 gcgcgaagtg cgccgcaatc tccgccgccg aatgcacggc aagataccgc cgcgggaatc
      841 cttcgaggaa ccgctcgatc tcttccgtgc cggcgacgct cgccagggcc ttcacgcgct
      901 ccagcaacga cacttcgtcg agcgcgtgca gtcggtcgcg atccagagtc cggctaaagt
      961 ggttggacgt ggcgacgaaa agctgccaca acatctccgc cttccagggc gtcagcgctt
     1021 ccggattcac ggaatgaatg tccgcatacg taagcaggca caatctttgc atgcgctcct
     1081 gcgttaccac ggactgcgca aaagcggata ccgtcgccgg atcgaagatg tcccggcgct
     1141 gcacggtcgc ggacatatcc aggtggtgct cgatgaggaa ttgtacttcc gctttttctt
     1201 ccggtgagag tttcaatcgc cgcgccgcgc tatccagcgc cgcgaggctg ccggttatgt
     1261 gattctctgc gggcatcccc ttgcccacgt cgtgcaacag cagcgacaag atcagcagat
     1321 cgcggcgctc gacggttttc cagagcggca cgaaatgcgt cccgcgagta tccggcgggt
     1381 ccgctagttc ctgaaggtgt tcgatggtcc gcagcgaatg ttcgtcgacg gtgtagcgat
     1441 gataaaaatc acgtactacc agcgaatcga tcatgccgaa ttccggcaga atctgggtca
     1501 gaagcccgag gcgttgcatc ggccgcaatg cgacgccggg ataatcggag ccaagaattt
     1561 cgtgcagagt gctccagggc atttccgtgt tgcgcggcgg gagttccgga tgcgtgagaa
     1621 tatagccgat gcttcgttct gcctcgcggc tcagcggggt tcctgtgcga gctgcttccg
     1681 cgaatagcga gtaggtcacg gcgcgatccg aaaacgccgg ctggtccacc acttcgagca
     1741 aaccatctcg gaccatgaaa taattcccgt ggtccggctc aaccctggag gcgcgcgccg
     1801 cattgagcag ccgttgtcgc aacgtcaaag ggactgggga tttctgttcg agatagcgca
     1861 gtagttgccg gttcagcgtt cgcgcgtgac ggaaatacag gcgcatccac tccgcggcgt
     1921 ttctggaaat gccatctccc acgccgagcg cacgttccgc tgctgccgcc tgcaattcgt
     1981 aggtgagcgt attatcgttc ctgccgttgc tgtaatgcag aaagcatcgg atggcgctaa
     2041 gaaagtcaac ggcagccgca gaaagttctt cctccgtcag cgtgattttc cggatttctt
     2101 ttttttcgtc aaggatctgc cgcagccaga ccgttgcctg ataatcccgc atgccgccgg
     2161 gcgactcttt cacgttcgct tcgaggtgaa agatggtatt tccgtagcgt gtcaatcgtt
     2221 cccgggtcag ccggtgaagt tgcgcgagca gaaaggggcg cgcctgcttt tccgacgccg
     2281 gcagcacttt cgtgtccagc ttctcgaaga ggccggagtc acctgccaga aatttgcgat
     2341 ccagcagcgc caggtggaat tccgcgttgt cttcttcgat ccgcttgcat tcttcaaggg
     2401 tccggcctgc cgagctcaca cggaatccca gatcccacag cgtccgggaa aactccgaga
     2461 tcagcgggcg cgattcctgt tcggctctct cgcttccaaa taaaaaaaga atgtcgagat
     2521 cggagtaggg aaagagcatc cgccgcccgt aaccgccaag cgcaagcagg caaagcccct
     2581 gcggttcccg gttgtggacc cgcaggaggt catcgaagat ttgctggatg ttgaggtccg
     2641 ccagttcgca aagcgcgcgc agggtttctc gcgcactcgt gcccgcctca aactgctgcc
     2701 ggattcgttg ccgttcggcg tcggcccgcg ggccgagcac ggacaagggc gtaggggaac
     2761 cttgcatgtt ttttgcggct gggatggccg gatcgaaacc ggggcgcgca gcggttgtca
     2821 cggtgatcgc ttcgcggtca caagcgagct gactgtttcg cagtcacacg cgagaggcta
     2881 gagcgcattt tcaccgcgct cctcattgcg aatccggatg gcttcctcaa ccttcgataa
     2941 gaatatctta ccgtcaccaa tctttcctgt cttggcggcc ttcaaaattg tgtccacggc
     3001 gcgattcacc agcgcatccg gaagcaccat ctccaatttt atcttgggaa gcaggtccac
     3061 ggtgtattcg cgcccgcggt agacttcggt gtgtcccttc tgccgcccgt gcccgcgcac
     3121 ttccgtcacc gtcattccct cgatgccgat ttcgcgcagg gcatctttca cggattcgaa
     3181 ccgcgaaggc tgaatgaccg cctcaatttt catcatggat gtttccgctc cgccctgtgc
     3241 ctgacgatgt cttgcccgct cccattatga ctccaggtta tatgcttcct caccgtgctg
     3301 cgtgatgtcc aatccttcaa tctcatgctc ttccggcacg cgcacaccgg tcagcaaatc
     3361 cacaatcttc agcaacacga tcgtaccaac tagcgccaag ccccacgcga tcagtatgcc
     3421 cacgagctga ttgccgactt gcccccagtg cccgtcgagc ccgcccacgg ctttgcctgc
     3481 gccaaaaata ggattgatga cctgctgcgc gaacaccccc gtgagcaacg cgccaatggt
     3541 tccgcccgcg ccgtgaacgc cgaaggcgtc gagcgcgtca tcatacccga agatggcctt
     3601 tacctttgtc accatcagat aacagagcac gcccgcgatc aatccgatca ccagcgccg
//
LOCUS       DASYVO010000544         3482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_13184, whole genome shotgun sequence.
ACCESSION   DASYVO010000544 DASYVO010000000
VERSION     DASYVO010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13184"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VGR03_18775"
     CDS             <1..666
                     /locus_tag="VGR03_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996386.1"
                     /translation="GGAEAHLKEYFSTLEEARADLMALWNSWDPKLKELGLVSDQESV
                     AKTMYDSAALAPMTQLRSIPKGDTLEEDHQRDRQLIVNFIRDKVPGAIEQFDRGGKTY
                     IQVQDYQKMRQGVGVLLAELMRIKAEGDYQAIKTLVDRYGVHFDAMLRDQVVARYKAL
                     DIPTYWAGINSKLTARAGKDGQIQGVDFSYPRDVIQQYLVYGSMYDSGLVPSVKPESS
                     AVR"
     gene            complement(674..1651)
                     /locus_tag="VGR03_18780"
     CDS             complement(674..1651)
                     /locus_tag="VGR03_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996387.1"
                     /translation="MFVQLRKVVVGVALLFAAVTAHAQSKVPPETRNAALRYWLAFAD
                     LQDPPADKATAELLEKTAAGEAAWDEAKLGPILGKNETAIWRMQRATKLPDCDWGLEY
                     DLGPRASIAYVSKARVLARLNTLDGMRLAAKGDSQKAVETWLAGIRFSQHLTRGGSLI
                     FSLVAKMGLISNFHSLTQAAQSGALKGEQRKQIEAAVRALPETGFDWGEALRYEEDPL
                     NVAVKQMAEATSLAAYYQEMMDKPAPKDFTLPKAPEIAAFHKLMNSAEEALRLPPDAA
                     SERLKTLQDSVKSLHPFFRDTTPSFTRINDARIEVQTARAQLLKALAGK"
     gene            complement(1671..2066)
                     /locus_tag="VGR03_18785"
     CDS             complement(1671..2066)
                     /locus_tag="VGR03_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996388.1"
                     /translation="MGQSKMKNNDKKFEEYLGEFEPRKPRALPERTVHRQVWPRRLAA
                     AAIAISLGASLWFVRRKNELNSGDIVAKNSTSMLETKSPGRPLALLPLTQLALQDPEQ
                     LDVQLNAASLRVLPDFRGSNSTLRVLAKE"
     gene            complement(2080..2559)
                     /locus_tag="VGR03_18790"
     CDS             complement(2080..2559)
                     /locus_tag="VGR03_18790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1996389.1"
                     /translation="MGGNKLSAEDVRRLYDQQGPALVAYARGFVTDAAAAEDAVHQVF
                     LRLLSAEITMPDAPVAYVYRAVRNAALNARRSGLKLVELDPQSSVFQHRGGNQEAALA
                     LEKTLAELPEEQREVVVMRVWSGLTLAEIAAAVGVPLNTVASRYRYALEKLRDKLLP"
     gene            2675..>3482
                     /locus_tag="VGR03_18795"
     CDS             2675..>3482
                     /locus_tag="VGR03_18795"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV1996390.1"
                     /translation="MKTSKPAKFGPQTQAIHGGEPNRHGVNGPIVTEIIRSSTFTFSS
                     TEEMKLWAEGKNKAYIYTRYGNPTLSVAEKKIAALEGAEAGVVAASGMAAISSALLAA
                     LKAGDELISTAEVYGGSYRLMRDVFPNMGITVRHVGTDLAGIENLVSPRSKVLYVETP
                     TNPTLRLVDIRKAVAFAKKHKLVSIIDNTFATPVLQNPIALGYDMVVHSATKALAGHS
                     DIIAGVAVGSEEWMKRVRHMIIYLGASMDPGAAYLLIRGIKTLGVRVERQC"
BASE COUNT          704 a         1107 c          899 g          772 t
ORIGIN      
        1 ggcggcgcgg aagcccacct caaggaatat ttctccaccc tggaggaagc ccgcgccgac
       61 ctgatggcgc tctggaactc gtgggatccc aagttgaagg aactcgggct ggtgtcggac
      121 caggagtccg ttgccaaaac aatgtatgac tccgcggccc tagctccaat gacgcagttg
      181 cgaagcattc cgaagggcga cacccttgaa gaagaccacc agcgcgaccg gcaactaatc
      241 gttaacttca tccgtgacaa ggtgccaggc gcaattgaac agttcgatcg tgggggcaag
      301 acctacattc aggtgcagga ctatcaaaag atgcgccagg gtgtcggcgt gcttttggcc
      361 gaactcatgc gcatcaaggc tgagggcgat taccaggcga tcaaaacctt ggtggaccgc
      421 tatggtgttc actttgatgc catgcttcgc gaccaagtcg ttgcgcgcta taaggcgctg
      481 gatattccca cgtattgggc gggtatcaac tcgaaattga cggcacgggc cggaaaagac
      541 ggacaaattc agggcgtaga tttcagttat ccgcgggatg ttatccagca gtatcttgtt
      601 tacggttcga tgtacgatag cggcctggtg ccatctgtga aaccggagag ctcagctgta
      661 cggtgatatg cgactactta ccggccagcg ctttcaggag ctgcgccctc gccgtctgta
      721 cttcgattcg ggcgtcattg atcctcgtga aggacggggt cgtatcccgg aaaaaaggat
      781 gaagcgactt aacggagtcc tgaagcgtct tcagccgttc gctcgccgcg tctggcggca
      841 gccgcagcgc ttcttcggcc gagttcatca gcttgtgaaa cgcagcgatt tccggagctt
      901 tcggtaacgt gaaatccttt ggcgcaggtt tatccatcat ctcttgatag taagcagcca
      961 gactcgttgc ctcggccatc tgcttcaccg ccacattgag gggatcttcc tcgtaccgca
     1021 gcgcttcgcc ccagtcgaat ccggtttccg gcaacgcacg cacggcggct tcaatctgct
     1081 tcctctgctc ccctttcaac gcgccactct gcgcggcctg cgtcagcgaa tgaaaattgg
     1141 aaatcagtcc catcttcgca accagcgaaa aaatcaggct gccgcctctc gtcaggtgtt
     1201 gcgaaaagcg aattcctgcg agccatgtct cgaccgcctt ctgcgaatcg cctttcgccg
     1261 ccagccgcat tccatccaag gtgttaagcc gcgccagaac tcgcgccttc gaaacgtagg
     1321 cgatcgaagc gcgcggcccc agatcgtatt ccaatcccca gtcgcagtcc ggcagtttcg
     1381 tcgcgcgctg catcctccag atcgcagttt cattcttgcc gagaatcggg ccgagtttcg
     1441 cttcgtccca tgcggcttcc ccggccgctg ttttctccag caattcagcg gtcgccttgt
     1501 ccgctggcgg atcctgcagg tccgcaaacg ccagccagta gcgcaaagcc gcgtttctcg
     1561 tctcaggagg aactttcgat tgggcatgtg ccgttaccgc agcaaaaagc agcgccacac
     1621 caacgacgac tttccgtagt tgtacaaaca ttctcggtcc tcctgtagga ctattctttc
     1681 gcaagcacgc gcagggtgct gttgctgcct ctaaaatcgg gaagcactct cagcgacgct
     1741 gcgttcaatt gcacatcaag ctgttcgggg tcctgcagcg ccaattgcgt cagcggcagc
     1801 aatgccagtg ggcgtcccgg cgatttcgtt tccagcatgc ttgttgaatt ctttgcaact
     1861 atgtcgccgc tgtttaattc gtttttcctc cggacgaacc acaatgaagc tcccagagaa
     1921 atcgctatcg ccgccgcagc aaggcgccgg ggccaaactt gtcgatggac agtccgttcc
     1981 ggcaaagcgc gcggcttccg cggctcaaat tcgccgagat actcttcaaa tttcttatcg
     2041 ttatttttca ttttcgattg ccccattttt cttccgcttc taaggcagta gtttgtcccg
     2101 cagtttttcc agcgcgtacc tgtaccgcga tgcgacggtg ttgagcggca caccaacagc
     2161 cgccgctatt tccgccagcg tcagtccgct ccacactcgc atcaccacta cttcgcgttg
     2221 ttcttctggc aactccgcca gcgttttctc gagggccagc gccgcttctt gatttccgcc
     2281 gcgatgctgg aatacgctac tctgcggatc aagctccacc agcttcagcc cgcttcttcg
     2341 cgcattcagg gccgcgtttc gaacggcgcg atatacatat gcgaccggcg catccggcat
     2401 ggttatttcc gcgctcagca ggcgcaaaaa tacctgatgc actgcgtctt ccgccgccgc
     2461 ggcatccgtc acgaaaccac gcgcatacgc caccagcgca ggcccctgct ggtcgtagag
     2521 ccgccgcacg tcctcagcgc tcagcttatt cccgcccaat cgtgccgtcc gttctgcgct
     2581 ccatctataa gacacgacag ccagcccaaa ttgtctatgc ccgtgccaaa ttggcggctg
     2641 tttttgttcg cccgcaaggt gcacactctt ccacatgaaa accagcaagc ccgcaaaatt
     2701 cggcccgcag acgcaagcca ttcacggcgg cgaacccaac cgccacggcg tcaacggccc
     2761 catcgtgacg gagattatcc gctcttccac ttttaccttt tcttccaccg aggaaatgaa
     2821 gctgtgggcc gagggcaaaa acaaagccta catctacact cgttacggca atcccacgct
     2881 ctccgtcgcc gaaaagaaaa ttgccgcgct cgaaggcgcc gaagccggtg tggtcgccgc
     2941 ttccggcatg gcagctattt cctccgcgct cctcgccgcg ctgaaagccg gcgatgaatt
     3001 gatctccacc gcggaagtct acggcggttc gtatcgcctc atgcgcgacg ttttccccaa
     3061 catgggcatc accgtccgcc acgtcggaac cgatcttgct ggcatcgaaa atcttgtttc
     3121 cccgcgttcg aaagttcttt acgtcgaaac gcccaccaat cccacgcttc gacttgtcga
     3181 tatccgcaaa gccgtggcgt tcgccaagaa acacaaactc gtctccatca tcgacaacac
     3241 ttttgccacg cctgtgctgc agaatcccat cgccctcggc tacgacatgg ttgttcacag
     3301 cgccacaaaa gctctcgcgg gccattccga tatcatcgcc ggcgtcgccg tcggcagcga
     3361 ggagtggatg aaaagagtcc gccacatgat tatctacctg ggcgcctcga tggaccccgg
     3421 ggctgcgtat ctccttatcc gtggcatcaa gactctcggt gtgcgcgtcg agcgccagtg
     3481 cg
//
LOCUS       DASYVO010000545        13456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_13191, whole genome shotgun sequence.
ACCESSION   DASYVO010000545 DASYVO010000000
VERSION     DASYVO010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 13456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13456
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13191"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /gene="pyk"
                     /locus_tag="VGR03_18800"
     CDS             <1..1007
                     /gene="pyk"
                     /locus_tag="VGR03_18800"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV1996391.1"
                     /translation="RNVICEVVNGGALGEHKGINLPGVKLRVPALTPKDRADLIFALR
                     HGADYIAVSFVRRAEDVLLAKQLIRRAGKDVPVIAKLEKPEAIENLDAILRAADGVMV
                     ARGDLGVEMNPERVPVVQKMIIARAREFRRPVITATQMLESMTENPRPTRAEASDVAN
                     AIFDGSDAVMLSAETASGKYPIEAVGMMARIIEEAEASIREFPRPAPQEKLKVAETVA
                     ELVCHASRELHMKLIAVFTHSGFTARLVSRYRPLVPIVAFSPEAETRRRMALIWGALP
                     RKIQDVQKVDGLAKVAEKRLLEERLVRKGDVIGIVAGTPMGIRGTTNFMKFHIIGGPA
                     "
     gene            complement(1680..2465)
                     /locus_tag="VGR03_18805"
     CDS             complement(1680..2465)
                     /locus_tag="VGR03_18805"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV1996392.1"
                     /translation="MGTSFGGRRYNFDWRSFITPGVKLLVLVCSGVFLLQTLVSLLAP
                     LGATIWLLKWFALIPEAVTHHIPPRIWQPFTYIFLHGGLFHLLINMLMLWMFGRELEL
                     VWGKRRFLNYFCICGVGAGLIELIVKTIPVFFGSLPSVIPTIGASGAIFGILMANAVL
                     FPDRRIWLIPLPVTIPMRPYVAVMGAIEFFSTLGSGGDNVSHVCHLGGMLVGYLYLRR
                     GSLLYNVRNTVTDWQHRRNRKRFEVYINKQKKEPPSRPDNWVN"
     gene            complement(2564..4804)
                     /locus_tag="VGR03_18810"
     CDS             complement(2564..4804)
                     /locus_tag="VGR03_18810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEV1996393.1"
                     /translation="MSERPWSSTAPPGEFRTYVPAEESPKEFTIRAVILGALFGLLFS
                     AVTVYVGLRAGLTVSASIPISVLSISILRAFGRSTILENNIVQTTGSAGESLAAGVMF
                     TIPALIFLGFGSEFTFWRIFPLALLGGWLGVLFMVPLRRQLIVKEHGNLVFPEGTACA
                     DVLVAGERGGSFAGRVFWGLGLGGVYTFLMNTVQAWTSQPEGRPSWFPGASFRAAITS
                     EYLGVGYIIGPRVSGILFAGGIISWLVMMPAIKFYGQLAGNTPIYPSTIPIPLMTPDD
                     IWRSYIRPMGAGAVAAAGLITLLRTLPTIISALRAGLKDVRAEGTGQSAASSRIDRDV
                     PMRWVILGSIVIIAMMWVLLTFHPMKDASTLWWQNLFAGIFVVVFGFLFVTVAGRISG
                     LLGNSSNPVSGMSIATLMATCAIFFLAGWTAPNYAVLALMIGGVVCIAAAIAGATSQD
                     LKTGYLVGATPFWQQMGLLVGVTVSTFAIGATLSLMNKGLEKYIPTPIPVSIEALPSG
                     VKIERDSFTYQNKSYVLINSLGSHEIPDGEYLYDAGTRQIEYQWAQGIGSDKAPAPQA
                     RLMATVINGILNQRLPWRLVLMGVALVIAVEILGVRSLAFATGSYLSIATTGAMFAGG
                     LVRWAVEASLKKKEGESTDSEASPGALYSSGLIAAGGVFGLLGIIINLLQDPEISNRV
                     PHWLTQILHLPWPEKLFAMGPKYWPLLALNDKLPMPTHAQSLFGVLMFALLATSLFYF
                     ARKKLD"
     gene            5150..7123
                     /locus_tag="VGR03_18815"
     CDS             5150..7123
                     /locus_tag="VGR03_18815"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEV1996394.1"
                     /translation="MVSYCRMVVLVLLLFVAAAVRRAQPVSAGDEWQPISQEELKMTS
                     MPEAPGAPAVYLYRQVDRDDSGRTPYEYKYARIKILTEEGRKYADVEIPFFKEEGNIR
                     SVKARTIRPDGSIVNFEGKAFDKTIVKAKGVKFLAKTFTLPDVQVGSIIEYHYMYDLN
                     QGYVYNSHWILSEELFTKRGKFSLKPNSDFALNWSWPAGLPAGTAPPKNEAGIVRLES
                     QNIPAFQVEDYMPPQNELKIRVDFAYSEGFSEKEPDKFWKKEGKKLNDRVESFAGKRK
                     AMEQAVAEIVVPNDTPEVKLEKIYAKVQKFRNTSWEMEKTEQEQKREKQKDINNVEDL
                     WKRGYGDGRQINWLFLALARAAGFEAYSVYISSRSEYFFNPQIMNAFQLNGDVVMVKL
                     NGKEVYCDPATAFAPFGLLSWPETGVKGLRLDKEGGSWVVTTLPPSSDSRTERKADLK
                     LTETGSLEGKLTVTFSGLEALWRRIEERNEDATNRKKFLEDQVKEYIPVGIEVDLTNQ
                     PDWRSSTQSLMAEFDLKVPGWVSSAGRRALVPVGLFSAPEKHAFEHANRVHPIYFSYP
                     SSKIDDVTIDLPLGWQVSSLPPVQDLDAKAALYSLKVENIKGTLHLTRRLSMDLLTLE
                     LKFYPTLRNFFQVVRTGDEQQIVLQPGATAASN"
     gene            7074..9113
                     /locus_tag="VGR03_18820"
     CDS             7074..9113
                     /locus_tag="VGR03_18820"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HEV1996395.1"
                     /translation="MNSRSSCNPAQRRRVIEMRRILIYLTFCVMGCLAGVPRSAAGDA
                     PGWMHALVNVALPAHDEKTDVVLLYSDRTVTVQSADKIKITVREAYKILRPGGRDYGT
                     VVASFNSHSKITGMRGWCIPAQGKDYEVKDKEAMEISLPKIDGSELVSDVKDKLLRIP
                     AADPGNIVGYEYEKEDQPFVLQDVWYFQRANPVRESHYTLQLPSGWEYKVTWMNYPEA
                     KPAQSGNQSQWVVSEVKGIKHEDDMPPWQGVAGQMIVSFFPPGGSSQNQGFQNWKQMG
                     IWYQGLTSGRRDASPELKQKVASLTSSASTPLAKMKAIGDFAQRDIRYVAIELGIGGW
                     QPHPAAEVFAHHYGDCKDKATLMGAMLHELGIDSYYVVINSERGSVTPETPAHMSGFD
                     HAIIAIQLPDGVTEGSLAAVMAHPKLGKILFFDPTDEMTPFGQLHGALQANYGLLVTP
                     DGGELIELPQLPPSMNGIQRTAKLSLSAGGTVSGEVQETRVGDRAWSQRWALRTVTKD
                     ADRIKPIETLLSHSLGTFQIMKASVGNLQLTDQPFMYNYSVVAQNYAKTAGNLLLVRP
                     RFIGNKSSDLLETKEPRKYPVEFDGPSRDTDTFEITMPPGYEVDDLPPPVNADYSFAS
                     YHSKTEVNGNTLKYTRAFEVKELSVPLSKVEDLKKLYRIIAGDERNTAVLKPAAH"
     gene            complement(9107..9622)
                     /locus_tag="VGR03_18825"
     CDS             complement(9107..9622)
                     /locus_tag="VGR03_18825"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEV1996396.1"
                     /translation="MTFTSDPRTRNSYSLALVRIGVGILFLIFGEYKVFGTAFTLHGG
                     FQFWINKFLEGGAYPFMAPILRGFVLAHATSIAFLVAYGELAIGLALVLGIMVRSASF
                     CGLLFMLTMLFSSDYPGAGAPFWQYFGASLSHSVFALCFAAFLVGRADSVWSVKAQRS
                     NHASGSVEGNQ"
     gene            complement(9636..10250)
                     /locus_tag="VGR03_18830"
     CDS             complement(9636..10250)
                     /locus_tag="VGR03_18830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996397.1"
                     /translation="MRFTEEDAGYVTVRPVVKQTFRLAELADMVVSVTGKNAPRVQQI
                     FRAGTVVYNGYRYWWEGFASEEDKIAGLLALFPDDDPSLPFNPAQVIAVSLEIGGGTQ
                     RSLVRITHREASARRLFHKRSVWEILLKAAQDSAPRYEKYSHAERADVYRLHLSSEMA
                     VSFLKEALNASPRGLRRKLASLQSPAALLFFIHRTNSVGAGAQS"
     gene            complement(10297..10752)
                     /locus_tag="VGR03_18835"
     CDS             complement(10297..10752)
                     /locus_tag="VGR03_18835"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEV1996398.1"
                     /translation="MSLAETIQKDLVDAMRAKEELRLSVLRGIKAAIKHKEVEKIRTL
                     DEAESIQILQTLVKQRKESIEQFTKGNRPELAEKETKELAIIESYLPAGASEAEMDAA
                     IAKAISESGANSIKQMGAVVKASKAALEGKTVDGKVLSDRVRDRLSKLG"
     gene            10924..12513
                     /gene="serA"
                     /locus_tag="VGR03_18840"
     CDS             10924..12513
                     /gene="serA"
                     /locus_tag="VGR03_18840"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEV1996399.1"
                     /translation="MKVIVADKISERGVELLKEQPGWNIVLTTKDTLNAEIADADALI
                     VRSATKVTAELLNQAKKLRAVGRAGVGVDNIDLEAATKRGVLVMSTPGGSSVSVAEHT
                     FALLLALVRQLPKFDAAMREGRWEKSSSGAEVRGKTLGLIGLGRIGSEVASRAEAFDM
                     RVVAYDPFISEAAARELSVELVPLDQLLAECDFISLHTAVSPQTREMINAESIAKMKK
                     GIRIVNAARGELINEADLAAAIKSGHVAGAALDVFAEEPPKNCPLIGLPNVITTPHVA
                     GSTAEAQEELGTQVALQIRDYLVEGVIRNAVNLPALSADQYRRVRPYLELAERLGSLV
                     SQAAASRPARIRIRYAGEVAEVGTHLLRSAVLAGVLNAVLDEKVNVVNAPAVAAARGL
                     TVVEETRRREHGFPNTLEVSALPEKTGSAAGFTAEGTVLHDGSPRVLQIEGIPLEAQL
                     EGTILYLRNRDEPGVIGQIGSTLGKLGVNIATFALGRREAQRGADAVSLVRIDGEVSA
                     SILGPIRAIPAITEAKLVRLG"
     gene            12804..>13456
                     /locus_tag="VGR03_18845"
     CDS             12804..>13456
                     /locus_tag="VGR03_18845"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV1996400.1"
                     /translation="MVSSKFGKVFFVILLICLFVNVSAYAQVGTTSVRGVVTDKTGAS
                     VGDAKVSLDNLGQAFHREMLTNDRGEYEFLALPPGTYALTVERAGFRKFEQKNLQLLV
                     NTPTTTNPTLEVGATSQTVEVSAQAVTLNTTDASLGNAFNENQVRQLPLEGRNVPDLL
                     SLQAGVVYTSNRADAPMDTDTRSGAVNGARSDQSNVTLDGVPVNDSGGHAFTSVLPVT
                     "
BASE COUNT         3141 a         3512 c         4214 g         2589 t
ORIGIN      
        1 gccggaatgt gatttgcgaa gtcgtaaatg ggggggcgct cggcgaacac aaggggatca
       61 acttgccggg agtgaagctg cgcgtgccag cgctgacgcc gaaagaccgc gctgatctga
      121 tctttgcgct cagacatggc gcggattaca ttgcggtgag cttcgtgcgg cgcgcggagg
      181 acgtgctgct ggcgaaacag ttgattcgcc gcgcggggaa agacgttccg gtgatcgcga
      241 agctggagaa gccggaggcg atcgaaaatc tcgacgcgat tctgcgggcc gccgacggcg
      301 tgatggtggc gaggggcgac ctcggcgtgg aaatgaaccc ggagcgcgtg ccggtggttc
      361 agaaaatgat tattgcgcga gccagggaat ttcggcgtcc ggtgattacg gcgacgcaga
      421 tgctggaatc gatgacggaa aatccgcggc cgacgcgcgc ggaagcttcc gatgtggcca
      481 acgcgatttt cgacggctcg gacgcggtga tgctgtcggc ggagaccgcc tcggggaagt
      541 atcccatcga ggccgtgggc atgatggcgc gaattatcga ggaggcggaa gcgagcatac
      601 gggaatttcc gcggccggcg ccgcaggaga aattgaaagt ggcggaaacc gtcgcggaac
      661 tggtgtgcca cgcctcgcgc gagctgcaca tgaagctgat cgcggtgttt acgcacagcg
      721 gattcacggc gcggctggtg tcgcgctacc ggccgctggt gccgatcgtc gcgttctcgc
      781 cggaagcgga gacgcggcgg cgcatggcgc tgatctgggg cgcgctgccc aggaagattc
      841 aagatgtgca aaaggtggac gggctagcca aggtcgcgga gaagcggctg ctggaagagc
      901 ggctggtgcg caagggcgac gtgatcggga ttgtggcggg cacgccgatg gggattcgcg
      961 ggacgactaa cttcatgaag tttcacatca tcggcggacc ggcgtgaaaa agcagtcagt
     1021 gatcagtcgt cagtgatcag ttctcagaaa ccaattgcgt ccggtcgagc tttttttgag
     1081 attttggcca tgccgtttcc ccctgactct cagcgacctg cccctcaatt cccagttatt
     1141 ggaccgagtg ccattgccaa aggggggtac cccacccccg tgttttttga cgcagagtgc
     1201 gcaattgctt gaaaagaaaa gagttgtgtt tttgaaggaa gcaaaaaagc gcaaaagagt
     1261 gcgcaagaat atgaaaaaaa aggggatagg ggatagggaa taggggctag agctcttgaa
     1321 gagtattgac cccctacccc cccaccaatt tgcatgaata ttaaaagaaa agacttacgg
     1381 aatgagcatt ttgcaaacag ttgattctaa tagatatgtt tgtgggtgtt tcggacaagc
     1441 ctaggcacaa caggcagccc ctaaaaaaag aaaagcggga gcaagctccc gcgctccaaa
     1501 tggagttatc tacagggtca attatgccag agcgagagaa aaagtcaagg aaaactttgc
     1561 agcgaaaact ttgacgggaa aactttgccg tgcgaacgag cggacaccgg caaccaactg
     1621 caaaggctaa ccttctagcc ggacacaact gagaactaag aactgagaat tgaacgctct
     1681 tagttgaccc agttgtctgg gcgcgagggc ggttctttct tttgtttgtt gatgtagacc
     1741 tcgaaacgct tgcgattgcg ccggtgctgc caatcagtga cagtattgcg gacgttgtag
     1801 agtaaagaac cgcggcggag atagagatag ccaacaagca tgccgccgag atgacagacg
     1861 tggctgacat tgtcgccgcc tgagccgagc gtggagaaga attcgatggc ccccatcacc
     1921 gccacgtaag gacgcatcgg aatagtgaca ggcaagggaa tcagccagat gcgccggtcg
     1981 ggaaagagca ccgcgttggc catgagaatg ccgaaaatag cgccagaggc gccaatggtg
     2041 ggaattaccg aaggcaagct tccaaagaaa actggaatcg ttttgacaat aagctcgatg
     2101 agccctgccc ccacgccgca gatgcagaaa taattcagaa agcggcgctt cccccaaaca
     2161 agttccagtt cacggccgaa catccaaagc atgagcatgt tgatcagcaa gtggaaaagg
     2221 ccgccgtgca ggaagatgta ggtgaagggc tgccaaatgc gcggagggat gtggtgggtt
     2281 acggcttcgg ggatcagtgc gaaccacttg aggagccaaa tggtcgcccc gagaggagcg
     2341 agcaggctga caagagtctg caaaaggaaa acgccggagc agaccaacac gaggagtttt
     2401 acgcctgggg taatgaaact tcgccagtcg aagttgtatc gccgcccgcc gaacgaagta
     2461 cccatattcg cagtgtacgg cggcgagtca aatccgtcaa agcaccctta tcgtacacat
     2521 tgcgcagacc gctccatgac gactagccag cttgctcgcc tgattagtcc agcttcttgc
     2581 gggcgaaata aaagagcgag gtcgcgagaa gagcaaacat gagaacgccg aagagtgact
     2641 gtgcgtgcgt gggcatgggc aacttgtcgt ttagcgcgag caagggccaa tacttagggc
     2701 ccatggcaaa gagtttctca ggccaaggaa gatgcaagat ctgggtgagc caatgcggga
     2761 cgcgattgga gatttccggg tcctgcagaa gattgatgat gatgccgagg aggccgaaga
     2821 cgcctccggc ggcgatcagg ccgctggaat agagcgcgcc gggactcgct tcgctatccg
     2881 tgctctcgcc ttctttcttt tttaagcttg cttccacggc ccagcggacc agaccgccgg
     2941 caaacatggc tcctgtagtg gcgatggaaa gataggagcc ggtggcgaag gccagagagc
     3001 gcacgccaag aatctccacc gcgatcacca aggcgacgcc catgagcacc agacgccaag
     3061 gtaagcgctg attgaggatg ccattgatga ctgtagccat caggcgggct tgcggcgcgg
     3121 gagctttgtc gctgccgatg ccctgtgccc attgatactc gatttgtctg gtacctgcat
     3181 cgtagagata ctcgccgtcg ggaatttcat gcgagcccag agagttgatc agaacgtagc
     3241 tcttgttttg gtaggtgaac gaatcgcgct cgattttcac gccgcttggc aaggcctcga
     3301 tgctgacggg aattggcgtg gggatgtact tctccaggcc tttgttcatc agactaaggg
     3361 tcgcgccgat ggcgaaggtg gagacggtga cgccgacgag aagccccatc tgctgccaga
     3421 aaggcgtcgc gccaacgaga tagcccgttt tcaaatcctg cgaggtggcg ccggcgatgg
     3481 cagcggcgat gcaaaccacg ccgccaatca tgagcgcgag cacggcgtaa tttggcgccg
     3541 tccatccggc gaggaaaaaa atcgcgcagg tggccataag agtggcgatg ctcatgccgc
     3601 tgacgggatt ggaagaattg ccgagcaggc cactgatgcg ccccgccaca gtaacgaaca
     3661 gaaatccgaa gacgaccaca aaaatacccg caaaaagatt ttgccaccag agggtgctgg
     3721 catctttcat cggatgaaac gtcagcaaca cccacatcat ggcgatgatg acgatcgagc
     3781 ccaggatgac ccagcgcatc gggacgtcgc ggtcgatacg gctggaggct gcggattggc
     3841 cagtgccctc ggcacgcacg tctttcaagc cggcgcgcag tgcggaaata atggtaggca
     3901 gagtgcgcag caaggtgatc aacccggctg ctgcgacagc gcctgcgccc atggggcgaa
     3961 tgtaactgcg ccagatgtcg tccggcgtca ttaggggaat gggaatggtg gaaggataga
     4021 tcggcgtgtt gccggcgagc tgcccgtaaa acttgatggc gggcatcatg accagccacg
     4081 aaatgatgcc gccggcgaac agtatgccgg agacccgcgg tccgatgatg tagccgacac
     4141 ccagatattc agaggtgatg gccgctcgaa agcttgctcc ggggaaccaa ctgggccggc
     4201 cttccggctg cgatgtccat gcttgaacgg tgttcatcag gaaggtgtaa acgccgccga
     4261 gaccgagtcc ccagaaaacg cggcccgcga aggagccacc gcgttcgcca gcgactagga
     4321 cgtcagcgca agcagtgcct tcgggaaaca ccagatttcc atgttccttg acgatgagct
     4381 gacgccgcag cggcaccatg aagagcacgc cgagccatcc gccgagaaga gccaatggga
     4441 aaatccgcca aaacgtaaat tccgaaccga agccgagaaa gatcagcgcc ggaatcgtga
     4501 acatgacgcc ggcggcgagc gattcgcccg ccgatccggt ggtctggacg atgttgtttt
     4561 caaggatggt ggagcggccg aaggcgcgaa ggatgctgat ggagagcacg gaaatgggga
     4621 tcgatgccga aacggtgagc ccggcgcgca agccgacgta caccgtcact gcactgaaaa
     4681 gcagcccgaa gagcgcgcca aggatgaccg cgcgaatagt gaattctttt gggctttctt
     4741 cggccggtac gtaggtgcgg aattcacccg ggggtgccgt ggaactccat ggacgttcgc
     4801 tcactgaatc ctccccattt gccggaatcg gatgccaata tggcccctgt gcggggggat
     4861 tatgccgcag gtgggcgagg tggggaagag aatcctcggc ggcggaaggg gatggggttt
     4921 cccaactcta ttgacagggg gatagcatcc ttgtagtgtc cggcggtata cgttgctcag
     4981 gtcagcaaca ctgcccatgg gacgggaccc tctaatagcg cgggcgaccg cggattcatc
     5041 aagaccactg gtgaccgcgg agcggtcaag cctccgatgt gcaggccccg gttctcttcc
     5101 tgcagttgtt ctaaattccg cattgctgcc aattcaaact gggaggccta tggtcagcta
     5161 ttgccgcatg gtcgtgctcg ttttgctgct attcgtggcg gccgccgtgc gacgcgcgca
     5221 gccggtaagt gcaggtgacg aatggcagcc catctcccag gaagaattga agatgaccag
     5281 catgccggaa gcgcccggag cgccggcggt ctatctgtac cggcaggtgg atagggacga
     5341 cagcggccgt acgccgtacg aatacaaata cgcgaggatc aaaatcctga cggaagaagg
     5401 ccgaaaatac gccgacgtgg aaatcccctt tttcaaagag gagggaaaca tccgcagtgt
     5461 taaggcccgg acgatccggc cggatggctc gatcgtgaac ttcgaaggga aggcctttga
     5521 caagaccatc gtgaaagcga aaggcgtaaa gttcctggcg aaaaccttca cattgcccga
     5581 cgtgcaggtg ggcagcatca ttgagtatca ctacatgtac gacctgaacc agggttacgt
     5641 ttacaactcg cactggatcc tgagcgagga actcttcacc aagcgcggca aattcagtct
     5701 gaaaccaaat tcggattttg ctcttaactg gagctggccg gccgggctgc cggcgggaac
     5761 cgccccgccg aagaatgaag cgggcatcgt ccggctggag tcgcagaata ttcccgcgtt
     5821 tcaggtcgaa gactacatgc ccccgcagaa cgagctgaaa attcgcgttg atttcgctta
     5881 tagcgagggc ttttcggaaa aagagccgga caaattctgg aagaaagaag ggaagaagct
     5941 gaacgacagg gtggaaagtt ttgcggggaa acgtaaggcg atggagcagg cggtggcgga
     6001 aatcgtagtt cctaatgata cgccggaagt gaaactggag aagatctacg cgaaggtaca
     6061 aaagttccgg aatacttcct gggagatgga gaaaacagag caggagcaga agcgggaaaa
     6121 gcagaaggac ataaataacg tagaggatct ttggaagcga ggttacggag atggccgcca
     6181 gatcaactgg ctgtttctgg cattggcgag agcggcggga ttcgaggcct actccgtcta
     6241 tatatcgtcg cgcagcgagt attttttcaa tccgcaaatc atgaatgcgt tccagttgaa
     6301 cggtgacgtg gtgatggtga agttgaatgg gaaggaagtg tactgtgacc cggcaacagc
     6361 gtttgctccg tttggattgc tgtcgtggcc cgaaaccggc gtaaagggac tgcgactcga
     6421 taaagagggc ggaagctggg tggtcactac gctgccgccg agttccgatt cgcggaccga
     6481 acggaaagcc gatttgaagc tgacggagac ggggtcgctg gaggggaagc tgacggttac
     6541 gttttccggg ctggaagcgc tttggcgaag aatcgaggaa cggaacgaag acgcaaccaa
     6601 ccggaagaag tttctcgagg accaagtgaa ggagtacatc ccggtgggga tcgaagtgga
     6661 tttgacaaac caaccggatt ggcgaagctc gacgcaatcg ctgatggccg agttcgacct
     6721 gaaagtgcca ggctgggtct cgtcagcggg gcggcgagcc ctcgtgccgg tggggctctt
     6781 cagcgctccc gaaaagcatg cgttcgaaca cgccaaccgc gttcacccga tctatttttc
     6841 ttatccatca tcgaagattg atgacgtgac gatcgacctg ccgctgggct ggcaggtgag
     6901 cagcttgccg cccgtgcagg acttggacgc caaggctgct ctttacagcc taaaggtaga
     6961 aaacatcaaa ggcacgctcc acttaacacg gcggctgagt atggatctgt taacgctgga
     7021 actgaagttc tatccgacgc tccgaaattt ctttcaggta gtgaggacgg gagatgaaca
     7081 gcagatcgtc ttgcaacccg gcgcaacggc ggcgagtaat tgagatgcgg cgcattctga
     7141 tttatttgac attctgcgtg atgggctgcc ttgccggggt gccgcggtcc gccgctggag
     7201 acgcgcccgg atggatgcac gcgctggtga atgtggcctt gccggcgcac gacgagaaaa
     7261 cggacgtcgt gctgctctat tccgatcgga ccgtaacggt tcaatccgcg gacaaaatca
     7321 aaataacagt tcgtgaagcc tacaagattc tgcggcctgg tgggcgcgac tacggcacgg
     7381 tggtcgcatc ctttaactct cattcaaaga tcaccggcat gcgcggctgg tgcatccccg
     7441 cgcagggcaa agactatgaa gtgaaagata aggaagcgat ggaaatttcg ctgccgaaaa
     7501 tagacggaag cgaactcgtc tccgacgtaa aagacaaact ccttcgcatt cccgcggcag
     7561 acccggggaa catcgtgggg tacgaatacg agaaggaaga tcagccattt gtcttgcagg
     7621 acgtttggta ctttcagcga gcgaatcccg ttcgagaatc gcactacacg ctgcagcttc
     7681 catcaggctg ggaatacaaa gttacttgga tgaattatcc ggaagccaag ccagcccagt
     7741 cgggaaacca gtcgcaatgg gtcgtgagcg aggtcaaggg gatcaagcac gaagacgata
     7801 tgccgccctg gcagggcgtg gctggccaga tgatcgtttc gtttttcccg ccaggtggtt
     7861 catcgcagaa ccagggattt cagaattgga agcaaatggg gatttggtat caagggctga
     7921 ccagcggacg gcgggatgct tctccggagc tcaaacagaa agtggcgtcg ctgacgtctt
     7981 ccgcgagcac tccgcttgca aagatgaagg ccattgggga ttttgcgcag cgggacattc
     8041 ggtacgtggc gatcgagctg ggaatcggcg ggtggcagcc gcaccccgca gcggaagtgt
     8101 tcgcccatca ttacggagac tgcaaggaca aagcgacgct tatgggagcg atgctgcatg
     8161 agctcggcat tgactcgtac tacgtggtga ttaattccga gcgcggttcg gttacgccgg
     8221 agacgccggc tcacatgagc ggtttcgatc acgccatcat cgccatccaa ttgcctgacg
     8281 gagttacgga aggttcgctg gcggccgtca tggcccaccc aaagctcggc aagattctgt
     8341 tttttgaccc gacagacgaa atgacgcctt ttggtcaact ccacggggcg cttcaggcga
     8401 attacggatt gcttgtgacg cccgatggcg gagagctgat cgaactgccg caactgccgc
     8461 caagcatgaa cggaattcag cggaccgcca agctcagctt gagcgcagga ggcacggtga
     8521 gcggcgaggt gcaggaaaca cgagtgggcg accgtgcctg gtcacagcgc tgggcgctgc
     8581 gaacggtgac gaaggatgcg gaccggatca agccgattga gacgctgctt tctcattcgc
     8641 tcgggacctt tcaaattatg aaggcgagcg ttggcaatct gcaactgacg gatcaaccgt
     8701 tcatgtacaa ctactctgtt gttgctcaga attacgcgaa gacggcggga aacctgttgt
     8761 tggttcgccc gcggttcatt ggcaacaaat cgagtgacct gctggaaaca aaggagccgc
     8821 gaaaatatcc ggtggaattc gacgggccgt cgcgagacac ggacacgttt gaaattacga
     8881 tgccgcctgg ctatgaagtg gacgacctgc ccccgccggt gaacgcggac tacagtttcg
     8941 cgagctacca cagcaaaacg gaagtgaatg gaaatactct gaagtacacg cgggcgttcg
     9001 aagtgaagga gttgagcgtg ccgctcagca aggtcgagga cctcaagaag ctctatcgta
     9061 tcattgcagg cgacgaacgg aacaccgcgg tgctaaaacc cgcggctcac tgattcccct
     9121 ccacactacc actcgcgtga ttgcttcgct gcgccttgac cgaccagaca gagtccgcgc
     9181 gtccgaccaa gaacgcggcg aagcagaggg caaataccga gtggctcagg ctggcgccga
     9241 aatattgcca gaagggcgct cccgcaccgg gatagtccga agagaagagc atggtgagca
     9301 tgaagaggag tccacagaag ctcgcggagc gcaccatgat gccgagaacg agggcgagcc
     9361 cgatcgccaa ttcgccatag gctaccagga aggcgatgct cgtggcgtgc gcgaggacaa
     9421 agccacggag aatgggcgcc atgaaggggt aagccccacc ctcgaggaat ttgttgatcc
     9481 agaattgaaa gccgccatga agagtaaatg ctgtgccgaa aactttgtat tcaccgaaga
     9541 tcaaaaaaag aatacccacg ccgatgcgga ccaaggcgag cgaatagctg ttgcgagtgc
     9601 ggggatctga agtaaatgtc atcgcgattc ctccttcatg attgagctcc ggctccaaca
     9661 gaattggtgc gatggatgaa aaatagcagg gcagcgggag actgcaggga tgccaatttt
     9721 cttcgcagac cgcgcggcga cgcgttcagc gcttctttca agaacgacac agccatctcg
     9781 gaagacaagt gcagccgata gacatcggcg cgttctgcat gggaatactt ctcgtagcgc
     9841 ggtgctgaat cttgtgcagc ttttagcaag atttcccaga cactccgctt gtgaaacagc
     9901 ctcctcgcgg aagcttcgcg gtgtgtaatt ctcaccagag aacgctgtgt gccgcctccg
     9961 atttcgagag agactgcgat tacctgggcc gggttgaaag gaagcgaagg atcgtcatca
    10021 gggaaaaggg caaggagtcc tgcaatttta tcctcttcgg aagcgaaacc ttcccaccag
    10081 tagcgatagc cgttatagac gacagtgccg gcgcggaaaa tttgctgcac acgcggggca
    10141 ttcttgcccg tcacgctcac taccatatcc gcaagttcgg ccaggcgaaa cgtttgcttc
    10201 acgaccggcc gcacggtcac atagcccgcg tcctcttcgg tgaagcgcat cgcgatggtt
    10261 tccgggagcg gcatgccgaa tgaataatga gaattctcag cccagcttcg agagacggtc
    10321 gcgcacgcgg tcgctgagta ccttgccgtc aacggttttg ccttccagag ccgctttgga
    10381 agctttcaca accgcgccca tttgcttgat ggaattcgcg ccggattcgg agatcgcctt
    10441 ggcgatggcc gcgtccattt cggcctcgct ggcgccggca ggcagatacg attcgatgat
    10501 ggcgagttct ttggtttctt tttcggcgag ttccgggcgg ttgcccttgg tgaactgctc
    10561 gatcgattcc ttgcgctgtt ttacgagcgt ttgcagaatc tgaatggact cggcctcgtc
    10621 gagtgtgcga atcttttcga cttccttgtg cttgatggcc gccttgattc cccgcaggac
    10681 gctgaggcgc agttcctcct tggcgcgcat ggcgtcgact aggtccttct gaattgtttc
    10741 cgcaagactc atcttgggct cctcatgtca ttgaatatag cagaccggga aatcagccgc
    10801 gaaggaatgg ttcgcgatga cttggcgagg tatcagccgt acttatttga cagggcgcga
    10861 caggctcgct actttggatt acttatactg tgcgggaaaa acccgcagga gacgacgggc
    10921 ctcatgaaag tcatcgtagc ggacaagatc agcgagcgag gcgtggagtt gttgaaggag
    10981 cagcccggct ggaacatcgt actgacgacg aaagatacac tgaatgcaga gatcgccgac
    11041 gcggatgcgc tgatcgtgcg cagcgcgacg aaagtcacgg cggaattgct gaaccaggcg
    11101 aaaaaattgc gcgcggtggg ccgcgcgggc gtgggcgtgg acaatattga cctggaagcg
    11161 gcgaccaagc gtggcgtgct ggtcatgagc acgcccggcg gaagttccgt cagtgttgcc
    11221 gaacacacct ttgcgctgtt gttggcactc gttcgccagc ttccaaaatt tgatgcggct
    11281 atgcgcgaag gccgctggga aaaatcgtcg tcgggcgccg aggtgcgcgg caaaactctg
    11341 ggtctcatcg gtctcggccg catcggcagc gaagtggcct cccgggcgga agcgttcgat
    11401 atgcgcgttg ttgcgtatga tccattcatc agcgaagcgg cggcacgtga actgtccgtg
    11461 gaactcgtac cgctcgatca acttctggcc gaatgcgact ttatctccct gcacacggcc
    11521 gtaagtccgc aaacgcggga aatgatcaac gccgagtcca ttgcgaaaat gaagaagggc
    11581 attcgcatcg tcaatgccgc ccgcggggag ctcatcaacg aagctgacct ggcggcggcc
    11641 atcaagagcg gccacgtcgc tggagctgcc ctcgacgtct ttgcggaaga gccgccgaaa
    11701 aattgcccgc tgatcgggct tccgaacgtg atcaccacgc cgcatgttgc gggttccacg
    11761 gccgaggcgc aggaagagtt gggtacgcag gttgcgttgc agatccggga ctatctcgtg
    11821 gaaggagtga ttcgcaatgc ggtgaatctg ccagcgcttt cggcggacca ataccggcgc
    11881 gtgcggccgt acctggaatt ggcggagcgg ctggggtcgc tggtttcgca ggcggcggcg
    11941 tcgcgcccag cacggattcg catccggtac gcgggcgagg tggccgaagt gggaacgcat
    12001 ttgctgcgca gcgctgtgct ggcgggcgta ctcaacgccg tgctggatga aaaggtgaac
    12061 gtggtgaatg cgcctgcggt ggccgcggca agaggcttga ccgtggtaga ggagacgcgg
    12121 aggcgcgaac acggcttccc aaatactctg gaagtctccg cgctgcccga aaaaaccgga
    12181 tcagcggctg gcttcacggc ggaaggcacg gttcttcacg acggctcgcc ccgcgtgctg
    12241 cagatcgaag gaattccgct ggaggcccag ctcgaaggca cgattctcta tttgcgaaac
    12301 cgcgatgaac ccggagttat cggccagatt ggaagcacgc taggaaagct gggcgtaaat
    12361 atcgcgacgt ttgctctcgg ccgccgcgaa gcccagcgcg gggccgacgc cgtctccctc
    12421 gtccgcattg acggcgaagt ctcagcctcg atcctcggtc cgatccgggc gatccctgcc
    12481 attaccgagg cgaagctggt tcgactgggc tagagctcgc tttccaattt cctggtacca
    12541 gcctctttat tcttatgaat ttgaatcgtg agtggaccgg atttgataaa caatttagaa
    12601 taacttgtct tgacatcctt cctcattctg tcctaaaagt tatcttaact ttagttcgat
    12661 taagtggtgg ctgggttgcc cggggaaaat tctcatttcg gcaccgtgac ttcgccttgg
    12721 cacattgccg gggctctctg cacccgtctg gcatccctgc ataagttttc ataccccaag
    12781 aaggtgcgcg aaaggagcag tttatggtgt catctaaatt cgggaaggtg tttttcgtaa
    12841 tcctgctgat ttgtttgttc gtcaatgtca gcgcctatgc gcaggtcgga accacgtcgg
    12901 ttcgcggtgt cgtgacggac aagaccggcg ccagcgtggg cgatgccaag gtgtcgctgg
    12961 ataatctggg ccaagcgttc caccgcgaga tgctgacaaa tgatcgtggt gaatacgaat
    13021 ttctagccct gcctccaggc acgtatgcgc tgaccgttga aagggcaggg ttccgaaagt
    13081 ttgagcagaa gaacctgcaa ttgctggtga acacgccgac gacgaccaat cccacactcg
    13141 aagttggagc cacgtcacaa acggttgaag tgtccgcgca agctgtaact ctgaacacga
    13201 ccgatgcgtc cctgggcaac gcgttcaacg agaaccaggt gaggcagctt ccgctcgaag
    13261 ggcgcaacgt tccggacctg ctcagtttgc aggcgggcgt ggtgtatacc agcaaccgtg
    13321 cggacgcccc gatggacacg gacacgcgaa gcggcgcggt gaacggcgct cgaagcgacc
    13381 agagcaacgt cacgctggat ggcgtgccgg tcaacgattc gggagggcac gcatttactt
    13441 cggtactgcc ggtgac
//
LOCUS       DASYVO010000546         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_194253, whole genome shotgun sequence.
ACCESSION   DASYVO010000546 DASYVO010000000
VERSION     DASYVO010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194253"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1900
                     /locus_tag="VGR03_18850"
     CDS             <1..1900
                     /locus_tag="VGR03_18850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996401.1"
                     /translation="EERWQTARDLAIELKWIAESGWQTGIPTSAARPMTSRERLTWAF
                     ALTATLIAAIFAFVYFNKPSPEVHVLRSMILPPDGGTFVFNGPIGGAYLSPDGRSVAF
                     IARVGKVTQLWVRPLDSFAARALPGTEDASFAFWSPDSRNLGFFAQGKLKRIAVVGGP
                     PQALCDADSSRGASWSRTEVIIFARVSGEIQRIPASGGTPQRVTTLDVTRHEGTHRWP
                     YFLPDGNHFLFMAALLGPVSEDNVFYLGSLDRKGSRILFHGSSPIAYAMGQVLYLADN
                     VLMARPFDLVKPDSTGEAVPIAEGVQFDRLFSNGIFSVSENGALLYQRGKGSSAHSLL
                     LIDRTGKRLGNLGESAPGSTPRFSPDGKKVVFDLISTNTGKVDLWIQDISSGNRTRMA
                     SDPLNLVSHFPVWSPDGTRLAYFSARTGNRAVYIKRVNQIAQEEERWEAKDDTFVGAG
                     DWTPDGKFLILTERPIRTAQQRIALLAVAGKDGAMPLLEVQGANVDSGQISPDGRWIA
                     YRSDESGKNEVYITSFPKPTGKLQVSIAGGITPRWRHDGKELYYLAPDRKLMAVELKD
                     TGGSLQVASTRPLFEMFPTMFLTAAGVNQYDVTQDGSRFVVDSVITDESSAPLSLVVN
                     WTAELKKK"
     gene            1897..>2152
                     /locus_tag="VGR03_18855"
     CDS             1897..>2152
                     /locus_tag="VGR03_18855"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV1996402.1"
                     /translation="MSLVAGTKLGPYEIVAPLGAGGMGEVYRARDTRLERTVAVKILP
                     ANFSADAERLRRFEQEARAAAALNHPNILAIYDIGTEGGSP"
BASE COUNT          439 a          616 c          661 g          436 t
ORIGIN      
        1 ggaagagcgc tggcagaccg cgcgcgacct ggctatcgaa ttgaaatgga tcgcagaatc
       61 cggctggcag acaggaattc caacctccgc agcgcggccg atgacaagcc gtgagcggct
      121 cacctgggcc tttgcgctga ccgcgacact catcgccgcc attttcgcgt tcgtttattt
      181 caacaagccg tcccccgaag tgcacgtcct tcgttccatg attcttccgc cagacggcgg
      241 aacattcgtt ttcaacggtc cgatcggagg agcctatctg tctcccgatg gccgcagcgt
      301 ggctttcatt gcgcgggtcg gaaaagtgac gcagctgtgg gtacggcccc tggattcctt
      361 tgcggctcgc gctttgcccg ggaccgagga cgcgtcgttc gccttttggt cccccgacag
      421 ccgcaatttg gggttcttcg cccaaggaaa actgaaacgt atcgcggtgg tgggagggcc
      481 gccgcaagcg ctgtgcgacg ctgactcaag ccggggcgcg agctggagcc gcacagaagt
      541 aatcatcttc gccagagtca gcggcgaaat ccagcgcatt ccggcttcgg gcggaacgcc
      601 gcaacgtgtc acgactctcg atgtaacccg gcacgaaggg acccaccgct ggccttattt
      661 cctgcccgat ggaaatcatt tcttatttat ggcagcgctc cttggccccg tcagtgaaga
      721 caatgtcttc tatttgggat cgctggaccg caaaggaagc agaatcctgt tccacggcag
      781 ttcgcccatt gcctatgcca tgggacaagt gctttacctc gccgacaacg tgctgatggc
      841 aagacccttc gatctcgtaa agccggattc cacgggagag gcggttccga tcgcggaggg
      901 cgtgcagttt gaccgtctgt tcagcaacgg aattttttct gtctcggaga atggcgcctt
      961 gttgtaccag cgggggaaag ggtcaagcgc gcattcctta ttgttgatcg accgtacagg
     1021 aaaaaggttg gggaatctcg gcgagtcggc gcctggttcc acgcccaggt tttcgcccga
     1081 tgggaagaag gtggtttttg acttgatctc tacaaacacc ggcaaggtag atctctggat
     1141 acaggacatc agctcaggaa accggacgcg catggcgagc gatcctttga acctggtgtc
     1201 ccatttcccg gtgtggtctc ccgacggaac gcgcctcgca tacttttccg ctaggaccgg
     1261 gaaccgcgcg gtttacatca agagggtcaa ccaaatcgcg caggaggaag agcgatggga
     1321 ggcaaaggac gatacgttcg tcggtgcagg ggattggacg cccgacggca agttcttgat
     1381 tctcacggaa cggccaatcc gcactgcgca gcagcgtatc gcgttgctcg cggttgcggg
     1441 taaggatggc gccatgccgt tgctcgaggt gcaaggcgcg aacgtggatt cgggccagat
     1501 ttcaccggac ggccgatgga ttgcctatcg ctcggacgaa tccgggaaga atgaagtcta
     1561 tattacgtcg tttccgaagc cgacgggaaa actgcaagta tcgattgccg gtggcataac
     1621 cccacgctgg aggcacgacg ggaaagaact ctactatctt gctccggaca gaaagttaat
     1681 ggccgtggag ttgaaagata ccggcggctc actgcaggtg gcctcgaccc ggccattgtt
     1741 tgagatgttt ccaacgatgt tcctgacggc agcgggagtg aaccaatatg acgtcacgca
     1801 ggacggcagc cggtttgtcg tagattcggt gatcacggac gaatcgtccg cgccgctcag
     1861 tctcgtcgtg aactggaccg ccgagctgaa gaagaaatga gtctcgtcgc aggcactaaa
     1921 ctcgggccgt atgagatcgt cgcaccgctg ggcgcgggcg gcatgggcga agtgtatcgc
     1981 gcgcgcgaca cgcgccttga gcgcacggtc gcggtgaaaa ttctccctgc aaatttctcc
     2041 gccgacgccg agcgcctgcg gcgcttcgag caggaggcgc gcgccgccgc cgccttgaac
     2101 catcccaaca ttctggccat ctatgacatc ggaaccgagg gcggctcgcc gt
//
LOCUS       DASYVO010000547        11481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_194330, whole genome shotgun sequence.
ACCESSION   DASYVO010000547 DASYVO010000000
VERSION     DASYVO010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 11481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11481
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194330"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VGR03_18860"
     CDS             <1..424
                     /locus_tag="VGR03_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996403.1"
                     /translation="THRWPLAPCKSPSGLAMDLKNRRLFSVCDEKVMAVVNADTGKVV
                     ATPVICEGPDASAFDPSTSYAFASCGDGNLTVIHEDSPDKYTIVENVPTKKSARTMGL
                     DLKTHNIFLPAADFDSPAPGERRGKMKPGSFVIVVVGK"
     gene            complement(523..1074)
                     /locus_tag="VGR03_18865"
     CDS             complement(523..1074)
                     /locus_tag="VGR03_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996404.1"
                     /translation="MVIATLLLLCSIPQMDDTAKMVNDSPAVISDSATKESTLVASLP
                     SAPAPKVKADPEPIAPNPAAQPFLPVKPVFTRPRETPRQRKIWYGLALAGHSGAAFDA
                     WSTHRAVVGGYGQEANPFLRPFAHSNAIYAATQVSPAFMDFLGKRMMVSQHGWLRKFW
                     WLPQAAGASMSFVSGAHNVGVVH"
     gene            1376..2065
                     /locus_tag="VGR03_18870"
     CDS             1376..2065
                     /locus_tag="VGR03_18870"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEV1996405.1"
                     /translation="MPGKDSSAAKRARVPQRSPQPPRFIVIEGPLRVGKTTLARILAE
                     RLHARRIYDCEDNPFLADFYKAKPGSSLRAQMYFLIERQKRLREALAVEAPGPVLSDF
                     LMEKDRIFANLNLDDEELKLYERYYEALTADIPAPDLVIYLQAKPEVLRARIAKKASR
                     DESHIAPEYIEEVARAYEHFFFRYAASDLLVINTSDIDFVERSEDLQLLLKRLQEPVK
                     GTQYFLPLGAE"
     gene            complement(2312..2794)
                     /locus_tag="VGR03_18875"
     CDS             complement(2312..2794)
                     /locus_tag="VGR03_18875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996406.1"
                     /translation="MIDESTKQAAQEYLAAKLTEEEQIYEEEHNLAMAVARSPLVWKH
                     IKDSIFAKCGEWNAVTQEETLTCKETAIGDVRIWCAARSKQMTVHYDSKKLLITVRNA
                     GRLEHEKDVIMRIEGYRTGPERTDRDVHLVRNEQAVNADMLIVGELRVLTGLSRQRGA
                     "
     gene            3154..3435
                     /locus_tag="VGR03_18880"
     CDS             3154..3435
                     /locus_tag="VGR03_18880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEV1996407.1"
                     /translation="MAMKNPPHPGGFVLRQCIVPFGLTIRDAAAALGVTRTTLSELPN
                     GKRGISPEMAVRLSKVFGGSAESWLTQQAQYDLAQVRADGIKLRRLALA"
     gene            4333..6813
                     /locus_tag="VGR03_18885"
     CDS             4333..6813
                     /locus_tag="VGR03_18885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1996408.1"
                     /translation="MAGNFQQDLRGGLRMLKKSPGFTFVAVLSLALGIGANTAIFTII
                     NAVFLHPLPVEEPSRLAEMFTRDTQTIDANTNFQLTPTSLPNYEDYRDQNTVFSGLAL
                     VTFPIPLNWGGQAEPQQLNASLVSGNFFDVLGVKPYRGRTFIADGDKKIGGNPEVVLS
                     YSLWARRFGSDDKLVGQTISLNGTSYTVVGVAPPNFKGVVLLGRPDVLWIPISMRDYV
                     LTGQLKDLENHRRFRWTSIVGRLKPQVGIAEARAAMKTIAAGLAKEYPRDNKGRTVEL
                     YPLNESALGINQRGQFSLAGGVLMGVVGLVLLIACVNLANLLLAQAAKREKELSIRAA
                     MGAGRFRLVRQLLTESTVLSLMGGLAGLLVAYWGRNVLWSFRPPFLLDGSIDLSFDAR
                     VLGFTVVISLLTGLVFGIIPAIKASGTDINEILKTGGRGGALGWTHNRLRGLLVISEI
                     ALALVALVGSGLFLRSMQNAQQYNPGFDSQNLFQMNFDLGALRYDEDHGQQFFRDVIE
                     RAKTVPGVAGASVSSNGIFGGGLLATIFREGEQTDPNNRGTLVNLDDVTPGHFETMRI
                     PLLSGRDFTDFDRENTTPVVIINEAMAKIVWPGQEALGKRFAIVTTPNLFQVVGLVGT
                     TIVGQIGEDPQPVAYFPMRQQYSPFATLVVRTTGNPEALLGTVRTQVQQIDKNLAFTN
                     GQTVQQILGQGLWAARMGAALLGLFGALALILASIGIYGVLAYSVAQRTSEIGLRMAL
                     GAQPHQVLRLVLKQGMFLALIGAVVGILVALPVARLASRLLYGVSATDPLTYAGITLL
                     LMGVALLACYLPARRATRIDPLVALRVD"
     gene            6930..8306
                     /locus_tag="VGR03_18890"
     CDS             6930..8306
                     /locus_tag="VGR03_18890"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEV1996409.1"
                     /translation="MKNKLLVAAVVAATIALAASLLAGAQVLPLPALVPTFLRWLAIL
                     LIAAYATSRRSLTTWIFVGLLAGAELGHDWPSVAVNLQLLGTIFLRLIKVIIAPLLFG
                     VLVVGIAGHSDLKKVGRLGIKSLIYFELVSTLAMLIGYAAIHISRAGEGVHLPPSSAA
                     QSLNVSPHTATQLITDIFPENIAKSVAEGQVLQIVIFSVLFAMGLALVPEPKRRPMLV
                     FAESLSETMFKFTNLVMYAAPLGVFGAVAYTVGHLGVGVLLPLLKLLATMYVALAFFI
                     VCVLLPIALWARIPIKRFIAAVAEPVTLGFATASSEATLPRAIEQLESFGVPREIVAF
                     VLPTGYSFNMDGASLYQSLALFFVVQAAGLHLSIGQQVLMILTLLVSSKGTAGVARAS
                     LLIVFGAATSFHLPMEPLFLLFAIDQLMDMGRTAVTVLGNCVACVVIARWEGEYPRES
                     SSPVEISA"
     gene            8552..9037
                     /locus_tag="VGR03_18895"
     CDS             8552..9037
                     /locus_tag="VGR03_18895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996410.1"
                     /translation="MKVFTRVFAAATLLFFFGAIKTQANDKRASLPASVVSAKTVYVD
                     NQTTSAELLNTAYTELSKWGRLQVVDTPQKADIVLRLSNGNYVRFVPGGENAPAADPK
                     AGKQNFAGADEAVPPGSTRISVIDPKTGNSLWSDIRKTDNAKAATHMLDGLRDAFDQR
                     K"
     gene            complement(9110..10645)
                     /locus_tag="VGR03_18900"
     CDS             complement(9110..10645)
                     /locus_tag="VGR03_18900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996411.1"
                     /translation="MIAPWANPEFIRNVRAQLRPGKVLATACISAAISIAVGFAFSHQ
                     GGVATGSYGWGRQLFQTAFWLQALMLAAGGGIACVNSIYKEKEQNTFDYQRVTQLTPL
                     ELALGKLFGAPVFTYFVGLCLMPLAIFGAVMGKQKVSIVLAVYVVMLVGSVTIHVLAL
                     LISLLTVRGSHTGAILLVLVLLWGTSSRIVPLQYFQVGPVGPFFAAQIAEWESWEPRT
                     VNLRDGTTYDVPGFYDVFFGKKVHHVPVLIAIDLLFAGWFLLALVRNIKRDPNYYQIY
                     SPLQALGFAMFLNLLFVAFFHWRAATPLECQSLLLTLNMVVFFGFGLVAMRNRESLRR
                     ILRRKEQPSLLDITWPAPLLILGTVAAGLLVVMGIAEGRDPQVEWNGSFAVLRSLFFA
                     VWIVRDIQYLQWISVRRGKHSLAMGVLFLGIFYVCIFILMAPLGIYTNAERTPFSAFF
                     VPSAAYLLDHGAWVLRPAIWGAAFVAQWALIAVFIGLQRQTMAELNSSANVSLPAPAP
                     VQP"
     gene            complement(10642..>11481)
                     /locus_tag="VGR03_18905"
     CDS             complement(10642..>11481)
                     /locus_tag="VGR03_18905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1996412.1"
                     /translation="FGLVGPNGAGKTTTLKMICGLMLPTAGRITVNNIDVEQEAEEVQ
                     QYIGYLADFFSVYDDLKTWEYVEHFARAYKLEPSKISARVREVIGVLGLETKYDAFVG
                     GLSRGMKQRLGIARAIIHDPPLLVLDEPASGLDPKARLEVKDLIRRLNREGKTIFITS
                     HVLSDLEEICTSLAIMEKGKLLRVGKIGDVMRGAGMTKRVRIRLALAGFALGSWLAAQ
                     PGVSETKDEGLEAEFVFPGTDAELAALVRAAVSAGAPICGVEEKVETLEALFSRLSSG
                     EVM"
BASE COUNT         2281 a         3648 c         3124 g         2428 t
ORIGIN      
        1 gacccatcgc tggcccctcg caccttgcaa gtcgccttca gggctggcca tggatctgaa
       61 gaaccgccgc ctgttctctg tttgcgatga aaaagtcatg gccgtggtga acgcggacac
      121 cggcaaagtc gtcgccacgc cggtcatctg cgagggcccg gatgccagtg ccttcgatcc
      181 ctcgacgagt tatgcgttcg cctcctgcgg cgatggcaac cttacggtca tccacgagga
      241 ttcgccggac aagtacacca tcgtcgagaa tgtccccacg aagaagtccg cccgcaccat
      301 gggtctcgac ctcaagaccc acaacatttt tctcccggcc gccgacttcg attcacccgc
      361 tcccggtgaa cgccgcggca aaatgaagcc gggttccttc gtcatcgtcg tggttggcaa
      421 ataggcggga atcacactca gggcgcgagc ctgcgcctgc caaaaaacaa aagcgccccg
      481 actggttcgg ggcgctttct gttttgggga tcgagggaac tcttagtgta cgacgccgac
      541 gttgtgcgcg cccgaaacaa acgacatgct cgcgcccgct gcctgcggca gccaccaaaa
      601 ctttctaagc caaccatgtt ggcttaccat catccgtttg cccaggaagt ccatgaacgc
      661 tgggctcacc tgcgtcgccg catagatggc gttcgaatgg gcgaaggggc gcaggaacgg
      721 attcgcctcc tgcccgtagc ccccgacgac cgcccggtgc gttgaccagg cgtcaaaggc
      781 cgcgccgctg tgtcccgcca gcgccagtcc ataccagatc ttcctctgcc ggggcgtttc
      841 gcgcggccgg gtaaacaccg gcttcaccgg cagaaaaggc tgcgccgccg ggttcggagc
      901 gatcggctcc gggtcggctt tcactttggg tgcaggcgcg gaaggcaggg aggcgacgag
      961 agtagattct tttgtcgcgc tatcggaaat cactgcggga gagtcgttaa ccattttcgc
     1021 cgtgtcgtcc atctgcggga tggaacacag caggagaagc gttgcaatga ccattggcca
     1081 cctcagtatt gcccgtgggt cttgcaagtc cgtccccact cgcaataccg ccactttact
     1141 ggtcaaaagg gcaggtagca ctggcccatt ggtcacattc tgtttagtac gtccctttag
     1201 ctcctccgtt tgccactttt aaaacggcag tactaggcca caaatgtgaa atttttttta
     1261 cattcctggc cctgcattgc ctcccatcga gagagagcga accgcagatg actctgtccg
     1321 cgactctgct ataatccgcg tgttgatccc tgccaccgct ggatcacacg aaacgatgcc
     1381 cggcaaagac tcttccgccg ccaagcgcgc tcgcgttccg cagcgcagtc cgcagcctcc
     1441 ccgcttcatc gtcattgaag gccctctgcg cgtcggcaaa accactctcg cccgcatcct
     1501 cgccgaacgc ctgcacgccc gtcgcattta cgattgcgag gacaatccct tcctcgccga
     1561 tttctacaaa gccaagcccg gctcctccct tcgcgcccag atgtacttcc tcatcgagcg
     1621 ccagaagcgc ctccgcgaag ccctcgccgt cgaagctccc ggccccgtcc tctccgactt
     1681 cctcatggaa aaggaccgta tcttcgccaa tctcaatctc gacgacgaag agctcaagct
     1741 ctacgaacgc tactacgaag ccctcaccgc ggacattcct gcccccgatc tggtcatcta
     1801 tctccaggcc aagcccgaag tcctccgcgc ccgcatcgcc aaaaaagcct cccgcgacga
     1861 atcccacatc gcccccgaat acatcgagga agtcgcccgc gcctacgaac atttcttttt
     1921 ccgctacgcc gcctccgacc tcctggtcat caacacctct gacatcgatt tcgtcgagcg
     1981 cagcgaagac ctccagctcc tcctcaaacg cctccaggag cccgtcaaag gcacccaata
     2041 cttcctcccc ctgggcgccg aataattgcg gttgtagctc aggccggaag ttgtcccgac
     2101 ccggtcggga ggcccgaggc tttttccctt ccgtcctctg cgagtttcac cccgccgtca
     2161 agatacaagg ggcgcagcac cgctgcgtcc tctcttagaa tttgttcttg tagggccagc
     2221 cttctgaggc gggccaatct tcctcgcttt gattctagac agagcggcgt gcggaaagtc
     2281 tgctcggtcc ttattcccta atccctaatc cctacgcccc tctttgccgg cttaatccgg
     2341 tcagaacgcg aagctcgccc acaatcaaca tatccgcgtt tacagcctgc tcgttccgca
     2401 ccaggtgaac atcgcgatcg gtccgctcgg ggcctgtccg gtatccttcg atgcgcatga
     2461 tcacgtcttt ttcatgttcg agccgccctg cgttcctcac cgtgatcaaa agtttcttgg
     2521 aatcgtaatg caccgtcatc tgtttcgatc gggctgcgca ccaaatccga acatctccaa
     2581 tcgccgtttc cttgcaggta agagtttctt cttgcgtcac cgcattccac tcgccgcact
     2641 tcgcgaaaat agaatcctta atatgcttcc aaaccagggg tgagcgcgcc accgccattg
     2701 ccagattgtg ctcctcttcg taaatctgct cttcttccgt cagctttgcg gccaggtatt
     2761 cctgcgctgc ctgctttgtg ctctcatcaa tcattaggcc cgtctcccgg tgaatgattt
     2821 tacatgatgt cgaatagcaa tcccttagtg atttggtgag cgggtggctt tgttcctttc
     2881 gtttggctct gccaaatagt gttctagcct ttgtaggggc gcagcaccgc tgcgttcgct
     2941 cttcgaattt gcatttgtag cggcgtccct ttagggcgcc atccttcttc ccgccttatg
     3001 tagcggcgac ttcacgtcgc catcctttcc cctgcttgta gaactccagc ctttgtagga
     3061 gcacaataca ttttgcccac cgccgacgcc cgctttgccg accggatcgc actgggcgtt
     3121 gattatgtcg attaccactg agagggttga accatggcga tgaagaatcc accgcacccc
     3181 ggcggctttg tgctgcgcca gtgcattgtg cctttcggct tgaccatcag ggacgccgct
     3241 gccgcgctgg gagtcacgcg gacgacgctg tcggaactgc ccaatgggaa gcgcgggatt
     3301 tctcctgaaa tggccgtgcg gttgtccaag gtttttggag ggagcgccga gagctggctt
     3361 acgcagcagg cgcagtacga ccttgcgcag gtccgcgccg acggcatcaa gctcaggcgg
     3421 ctggccttgg cttagtcggc ttcaaagagc ttcgtttggg cgatagacat tccaagactt
     3481 gcccgcccgt tttgactagc gcacgcgacc cagatcagct tcgggccggg gcctggagcc
     3541 acttgatggc tctacgccag tgggcgactt ctccgccgct catgggcgtc tgcaaccaca
     3601 cgattaggct gtgccgtgta accgcccatt ggctcggacc gctctcatgg agctccggcg
     3661 ggacaacagg gggtatttcc gccggcgtaa gccaataggg gctcgtgccc gcaaaggatc
     3721 gctcggcgac ggcactggcc gagtgtcgct ctgggatcgc acccgtaacc gacagcttta
     3781 gtagcgcccc gacctgctcc tgggacgccc tcatcgtttt caggattttg cgcgcaaacc
     3841 agacacctgc agctagaaag gcaatttgta tgaggagatt tcccagcgca taccaattgc
     3901 tcagcatcca agcggtaaat tcgttcatgg cctcaccttg gactgggata ggctcaactt
     3961 actactcggc tccgacggag gaaactggta ggaagtccag aaaacgggca ggtggtttcc
     4021 ctgccctggc ggcccaccct tcagcctggc gaaacatggg gcagtcggcc ggtgggaacc
     4081 tgcagttctt gcggtcgcca atcgcccgct gaaggaatga tgacgcggag aatgaagatc
     4141 aaattcccca ggcgcaaaac tagcgcccgg ggcacccggc tgccagactg ttctttatgt
     4201 gttaggtaca tattcatcct tcaccatagc gacggtctgc ctacctcttc gtgtttggta
     4261 ctgaaaaagg ggccggaagc tggaaacaaa tgtccttttt ggccgtccta accgttgagt
     4321 gggggtgacg tcatggctgg aaacttccaa caggatctgc ggggcgggtt gcggatgctg
     4381 aagaaaagcc ccggctttac gttcgtggcc gtgctgtcgc tggcgctggg catcggggcg
     4441 aacactgcca tcttcacgat aatcaacgcg gtcttcttgc acccgctgcc cgtcgaggag
     4501 ccctcgcggc tggctgaaat gtttacgcgc gatacccaaa cgatcgatgc gaataccaac
     4561 ttccagctga ccccgacttc gctccccaac tacgaagatt accgcgacca gaataccgtt
     4621 ttctccggac tggcgctggt caccttcccc atacccctga actggggcgg ccaggcggag
     4681 ccccagcagc taaacgcctc gctagtgagc ggaaacttct tcgacgtgct gggcgtgaag
     4741 ccctatcgcg ggcgcacctt catcgccgat ggagacaaga agatcggcgg caacccggaa
     4801 gtggtgttga gttacagctt gtgggcgcgg cgattcggtt ccgacgacaa gctggtcggc
     4861 cagacaattt ctctaaatgg gacatcgtac acggtcgtgg gcgtggcacc gccgaatttc
     4921 aaaggagtcg tgttgctcgg ccgtccggat gtgctctgga tccccatcag catgcgcgac
     4981 tatgtgctta ccgggcagct caaggatctc gagaatcacc gccgctttcg ctggacctcc
     5041 atcgtcggcc ggctgaaacc gcaggttggc attgcggagg cgcgggcggc catgaaaaca
     5101 atcgcggccg gcctggcgaa ggagtatccc cgcgacaaca agggccgcac cgtggaactc
     5161 tatccactga acgaatcggc gctggggatc aatcagcggg gacagttctc tcttgccgga
     5221 ggcgtgctga tgggcgtggt cggcttggtg ctgctgatcg cgtgcgtcaa tctggccaat
     5281 ctgctccttg cgcaggcggc caaacgcgag aaagagttga gcattcgcgc ggcgatgggc
     5341 gcggggcgct tccgcctggt ccgtcagctt ttgacggaaa gcaccgtgct ttcgctgatg
     5401 ggcggtttgg ccggtctgtt ggtggcgtac tggggacgca atgtgttgtg gtccttccgg
     5461 cctccgtttc ttctcgacgg ctcgatcgat ctttcgttcg atgcccgagt gctgggattc
     5521 accgtggtca tctccctgct gaccgggctg gtcttcggga tcattccggc gatcaaggct
     5581 tccggcacgg acatcaacga aattttgaaa actggcggcc gcggcggagc gctgggctgg
     5641 acccacaatc gtttgcgagg cttgctggtg atttcagaaa ttgcgctggc actggtggcg
     5701 ctggtcggct cgggactgtt tctgcgcagc atgcagaacg cgcagcaata caatcccggc
     5761 ttcgactcgc agaatctgtt tcaaatgaat ttcgacctgg gcgcactgcg ttacgacgag
     5821 gatcacgggc agcagttctt ccgcgacgtc atcgagcgcg cgaaaaccgt ccccggcgtt
     5881 gcgggcgctt cggtatcctc gaacggcatt ttcggtggcg gcttgttggc caccatcttt
     5941 cgcgaagggg aacagaccga tcccaataac cgcgggacac ttgtaaatct tgatgacgtg
     6001 acgcccggcc acttcgagac catgcgcatt ccgcttttga gcgggcggga cttcacggac
     6061 ttcgaccgcg aaaacaccac gccagtagtc atcatcaacg aggccatggc caagatcgtg
     6121 tggcccggcc aggaagcgct cggaaagcgc ttcgcaattg ttactactcc aaacctcttc
     6181 caggtggtgg gccttgtggg aaccactatc gtaggccaga tcggggaaga tccccagccg
     6241 gtggcgtact tcccgatgcg gcagcaatac tcgcccttcg cgacgctcgt cgtgcgcacc
     6301 acaggcaacc ccgaagctct tctcgggacc gtgcgcacgc aggtgcagca aatcgacaag
     6361 aatctggcgt ttaccaacgg gcagaccgtg cagcaaattc tggggcaagg gctgtgggcc
     6421 gcgcgcatgg gcgcagcatt gctcgggctg ttcggtgcgc tcgcgctgat cctcgcttcg
     6481 attggaattt atggcgtgct ggcatattcc gtggcgcagc gaacgagtga aatcggactg
     6541 cgtatggcgc tgggcgcaca accgcatcaa gtgctgcggc tggtgcttaa acagggcatg
     6601 ttcctggcgc ttattggcgc ggtcgtgggg atattggtgg cgttgccggt cgcccgcctg
     6661 gcgtcgcgcc tgctgtatgg cgtgagcgcg accgatcctc tcacttatgc gggaatcacg
     6721 ctgctgctga tgggcgtcgc gctgttggct tgctaccttc ccgcacggcg cgccacacgc
     6781 attgatccgc tcgtcgcatt gcgcgtggat tagcggccgt tgcgttgcgt ctggaatagc
     6841 ggccagctct ttccgccgcg cgtctccatt gacatgcggc gctaaccagc atacctttcg
     6901 gcaccaatgg aatccttcgc caatcgccga tgaaaaacaa acttctcgtc gcggcagtcg
     6961 tcgccgcaac gattgccctc gcggcatcgc tcctcgcagg cgctcaggtg ctgccgctgc
     7021 ccgcgcttgt cccaacgttc ctgcgctggc tggcgatcct gctcatcgcg gcctatgcca
     7081 catcgcggcg ttcgctgacg acttggatct tcgtcggcct actcgcgggc gcggagctgg
     7141 gccacgactg gccttcggtc gcagtcaact tgcagctcct cggcaccatt tttctgcggc
     7201 tcatcaaggt catcatcgcg ccgctgctct tcggcgtcct ggtcgtcggc atcgctggcc
     7261 actccgactt gaagaaagtc ggccgcctgg gcatcaagtc gctcatctac ttcgagctcg
     7321 tctccaccct cgccatgctg atcggctatg cggccattca tatcagccgc gcaggagaag
     7381 gcgtccatct ccctccgtcg tcagccgcgc aatcactcaa tgtttcgccc cacaccgcca
     7441 cccaactcat caccgacatt ttcccggaga acattgccaa atccgtcgcc gaagggcagg
     7501 ttctgcaaat cgtcatcttc agcgtcctct tcgcgatggg cctcgcgctc gttccggagc
     7561 ccaagcgccg ccccatgctg gtcttcgccg aaagcctctc ggaaaccatg ttcaagttca
     7621 ccaacctcgt catgtacgcc gctccgctcg gcgtctttgg cgccgttgcc tacaccgtcg
     7681 gccatctcgg agtgggagtc ctgcttccac tcctgaaact tttggccacc atgtacgtcg
     7741 cgctggcctt tttcatcgtc tgcgttctgc tgcccatcgc gctgtgggcg cgcatcccca
     7801 tcaagcgctt cattgccgcc gtcgccgagc ccgtcaccct cggatttgcc accgccagct
     7861 cggaggccac actgcctcgc gccatcgaac aactggagtc cttcggcgtt ccgcgcgaaa
     7921 tcgtcgcctt cgtcctcccc accggctaca gcttcaatat ggacggcgcg agtctctatc
     7981 aatctttggc cttatttttt gtcgtccagg ccgcgggact gcacctcagc atcggtcagc
     8041 aagttttgat gattctgacg ctgctggtca gcagcaaagg gactgccggc gtggcgcgcg
     8101 cttcgctgct gattgttttt ggagcggcaa cttcgtttca tttgcccatg gaaccgctgt
     8161 ttcttctctt cgccatcgat caactcatgg acatgggccg caccgcggtc actgttctcg
     8221 gcaattgcgt cgcttgcgtc gtcatcgcgc gctgggaagg cgagtacccg cgcgaatcct
     8281 cttcgcccgt cgaaatctcg gcatgaactc tcgcttccgt ggaacagggg cggaaatcgc
     8341 gaagcgcacc acaagattcg gcttattgcc cttcgggaca gggctcatat atcgccgcgc
     8401 catctgcact ttttctcgtc ttcagtccag ttacgggccg ccctcgccag tcatagactg
     8461 cccgcacaat cgatacctag agaagcatga cccggccagc cgtaggggcc gctcggtcac
     8521 agaaattctg agcggcaagg agtgtgtcca catgaaagtt ttcacgcgcg tatttgcggc
     8581 cgccactctc ttgttttttt tcggcgcgat aaaaacacag gctaatgaca aacgtgcttc
     8641 actcccggcg agcgttgtct ctgcgaaaac ggtttacgtc gataaccaaa cgacttccgc
     8701 cgagctactg aatacggctt acacggaact ctcaaaatgg ggacgccttc aggttgtcga
     8761 cacgccgcag aaagcagata tcgttctgcg cctctcgaac ggcaattacg tgaggttcgt
     8821 ccccggcggg gaaaacgccc cggctgcaga ccccaaagcc ggcaaacaaa actttgcggg
     8881 cgcggatgaa gcggtgccgc cgggttccac gcgcatttcg gtgatcgatc ccaagaccgg
     8941 caactcgctt tggtccgaca ttcgaaaaac ggataacgca aaagccgcaa cgcacatgct
     9001 cgacggcctc cgcgacgcct tcgatcagag aaaataagcg cacgatggaa aatggcaccc
     9061 ggatcttgac gcgcaacgct ttccaacacc tcattagatg gaaaaacttc tacggctgga
     9121 cgggagcggg agccggcaag gagacgttcg cagaagaatt cagctccgcc atggtttgcc
     9181 gctgcagccc gatgaacacg gcgatcaaag cccactgggc aacaaatgct gctccccaga
     9241 tcgccggccg caaaacccac gcaccgtggt ccaacaaata tgccgccgag ggcacgaaga
     9301 acgcggaaaa aggagtgcgt tccgcattcg tgtaaatgcc cagaggagcc attaaaataa
     9361 atatgcatac gtaaaaaatc cccagaaaaa ggacgcccat cgccagggaa tgttttcccc
     9421 gccgcacgct gatccattgc aggtactgga tgtcgcgcac gatccatact gcaaaaaaca
     9481 gcgatcgcaa caccgcaaaa cttccattcc actcgacctg cgggtcgcgc ccctcggcga
     9541 ttcccatcac caccagtagc cctgctgcga ccgtgcctaa gatcagcaac ggcgccggcc
     9601 acgtgatgtc caacaacgaa ggttgctctt tcctccgcag aatccggcgc agagactcgc
     9661 gatttctcat cgccaccagg ccgaaaccga agaacacgac catattcagc gtcagcaaaa
     9721 gcgactggca ttcgaggggc gtggcggctc tccagtgaaa aaacgcgacg aagagcagat
     9781 tcagaaacat cgcaaacccc agcgcctgca gcggcgaata aatctggtag taatttggat
     9841 cacgcttgat atttcgcacg agtgcgagca aaaaccagcc ggcgaaaaga agatcgatgg
     9901 cgattaagac cggaacatgg tgaacttttt ttccaaagaa aacgtcataa aacccgggaa
     9961 cgtcgtaggt ggtgccatct cgcaggttca ccgttcgtgg ttcccagctc tcccattccg
    10021 ctatctgcgc cgcaaaaaat ggccctacgg ggccaacctg aaaatactgt aaaggcacaa
    10081 ttctggagga cgtgccccag agaagaacaa gcacgagcag gatcgccccg gtatgtgacc
    10141 cgcgaaccgt caagagcgaa atcagcagcg ccaatacgtg tatcgttacc gaaccaacca
    10201 gcatcacgac gtagaccgcc aacacgatcg aaaccttttg tttccccatg acagcgccga
    10261 agatggccag cggcatgagg cagaggccca caaaatacgt aaagaccggc gcgccaaaaa
    10321 gttttcccag cgccagctcc agcggcgtca attgcgtcac tcgctggtaa tcgaaggtgt
    10381 tctgttcttt ttccttgtaa atgctgttga cgcaggcgat gccgccgccg gccgcgagca
    10441 tgagtgcttg cagccaaaat gcggtttgga acagttgccg gccccaccca taggatcccg
    10501 tcgccacgcc gccctgatgt gaaaacgcga agcccacggc aatggaaatc gccgcggaga
    10561 tgcacgccgt ggcgagcact ttcccgggcc gcaattgcgc tcgcacgttg cggatgaatt
    10621 ccggattggc ccacggcgct atcacattac ttctccactc gagagcctcg agaacagcgc
    10681 ttccagagtt tccactttct cttccacgcc acagatcggc gcgcccgcac tcaccgctgc
    10741 acgcaccagc gccgcaagct ccgcatctgt tcccggaaaa acaaattctg cctcaaggcc
    10801 ctcgtctttg gtttcgctga caccgggctg cgcggcaagc cagcttccga gcgcaaatcc
    10861 tgccaaggcc aatcgaatgc gcacgcgctt ggtcatccct gctccgcgca tcacgtcgcc
    10921 gatcttcccc acacgcagaa gttttccttt ttccatgatc gcaagcgagg tgcaaatctc
    10981 ttccaagtcg gagagtacgt gcgaagtgat gaagatcgtc tttccttcgc gattcagccg
    11041 tcggatcaaa tccttcacct ccagacgtgc ttttggatca agccccgacg ctggctcgtc
    11101 cagcaccagc aatgggggat cgtgaatgat ggcgcgcgcg attcccaggc gctgcttcat
    11161 cccgcgcgag aggccgccta caaacgcgtc atacttcgtt tccagcccga gtactccgat
    11221 aacctcgcgg acgcgcgcgg aaattttcga aggctccagt ttgtaggccc gcgcgaaatg
    11281 ctcgacgtat tcccaggtct tcaggtcatc gtagacggag aaaaaatccg ccaaatagcc
    11341 aatatattgc tgcacttcct ccgcttcctg ctccacgtcg atattgttta ccgtgatgcg
    11401 tccggcggtg ggcagcatca ggccgcagat cattttcagt gtggtggtct ttcccgctcc
    11461 gttcggcccc accaggccaa a
//
LOCUS       DASYVO010000548         6288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_194370, whole genome shotgun sequence.
ACCESSION   DASYVO010000548 DASYVO010000000
VERSION     DASYVO010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6288
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194370"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VGR03_18910"
     CDS             <1..372
                     /locus_tag="VGR03_18910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996413.1"
                     /translation="LLSGPQIDPGFAGVLVIRVVNLAPKPVVLPYQDPFLTVQFFRLS
                     APVVAPYSGPRQGQTGISGRDLQELVATEGLTLGQVIKTLTALAKDVAELRGSIGRLS
                     WSVPLIVAVGMAIVGIIAAIK"
     gene            complement(365..1180)
                     /locus_tag="VGR03_18915"
     CDS             complement(365..1180)
                     /locus_tag="VGR03_18915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HEV1996414.1"
                     /translation="MNLLPKEKQIQIINALVEGNSIRATARMVDVEHKTVMRVLLRVG
                     ANCAQILNQRMRQLPCRFVQADEIWTYVGKKERRMVQGDNPAEVGDQYVFVAMDAETK
                     LVPCFRVGKRNAENTWYFVQDLQGRLANRVQLTTDGFRPYLPAVEDAFGANVDYAMLI
                     KMYSESGLADKRYSPGEIVNALPIPVSGNPKPYLISTSHIERQNLTIRMQLRRFTRLT
                     NAFSKKLENLKAACALHFAWYNFCRIHQTLRVTPAMEAGLTERVWGSEELLAI"
     gene            1415..2470
                     /locus_tag="VGR03_18920"
     CDS             1415..2470
                     /locus_tag="VGR03_18920"
                     /inference="COORDINATES: protein motif:HMM:NF022021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HEV1996415.1"
                     /translation="MGDQTIQSKGGDARAAKLTAAERKQIARKAAESRWGADLPQASH
                     DGPLHIGDAVLIAANLPNGKRLLSQTTMLTAIGRSPNPPAGTGVLATLDGLPSFLRAE
                     VLKPFISEELMLSTTPIHFKLKNGTRTVGYDAMLLPMVCEVYLRLRDHLSSRLADGDA
                     AEKRRARSVLRQYKHIVEACDRLTRGLARRGIIALIDDATGYQADKAREEVQRIIQAY
                     VAPALVPWTRKFPHEFFREVYRLLGWEYRPGQIKHPQYMGRFINKYVYEALPPGVLDE
                     LKRRLPKNEHGNRRAKLWQLLTVDTGSPHLDKQLTADITLMQVSDSKAQFEDNFEKVF
                     GMQPRLPFGTPTKELKG"
     gene            2643..3419
                     /locus_tag="VGR03_18925"
     CDS             2643..3419
                     /locus_tag="VGR03_18925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996416.1"
                     /translation="MSKRSVGLVVIVLLVFSAPVALSQMEQAHPVYTFVSQFQVPRAQ
                     WAQYAEDTEKNANPIFERLMADGTIVGWGNFEQIVHTPDGMTHGAWWSSTSLAGITRV
                     LDEIRKGGPRPGQIAATKHEDFLMRSVYSHTSAVSGGGAGYLRVNCTLTQPGKSDDFA
                     ATIKKHLGPTFEDQFKKGIATSYGMDEQFIITEPGSMRCTVVTFSNAENMNKWAEAIA
                     ATLDKMSPEERKGWQEGILNTTVPDSRRDILARITHSAHK"
     gene            complement(3826..>6288)
                     /locus_tag="VGR03_18930"
     CDS             complement(3826..>6288)
                     /locus_tag="VGR03_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996417.1"
                     /translation="PQGSSPGQLGQLWRSDDGGDNWQMVNSDRQLRGRTHYYTREEIA
                     PDNENEVFFFSSSFSRTLDGGHTLTKMPASPGGDNHEMWIDPTNGDRMAVVNDGGINL
                     SVNRGRSWNHVNLPIAQIYHVTVDDQIPYFVYGNRQDGPSWRGPSNSLQFGGFFGNSI
                     PRGAWHPVAGGESGFAVPDPVDNNIIWSTGTGTGSVGGTVTRFDERTRQNREVEVWPD
                     YVAGAPAAEVKYRFNWEFPIAISPHDHNKIYVGSQYVHVTTDGGNSWQIISPDLTRND
                     KSRQQISGGLTPDNIGVEYAGVIFALTESPKEAGLIWAGTNDGQVQVTRDGGKNWSNV
                     TKNLPNFPEWGTVDNIEASRYDAGTAYLTVDGHQVNNRDPFVYKTADYGKSWTAITAG
                     VPHNMLSYAHCVREDPARKGMLYLGTEGGLYISFDDGKNWQPLQAGLPHAPVYWLTVQ
                     ERFHDLVVATYGRGFWILDDITPLEQMASEVTASKAHLFAPRDAYRFKEISQPGAVEY
                     DPTAGKNPPYGASINFYLKSNLGEKDLAKLTIADAGGKTVREIECRAPKPGAAEAKAP
                     SEDEDGDAEPRPCEVKAGINRVWWDLRSERSTEIRLRTTPLYAPDVPFGPEGWRKLPA
                     IGRLAVLALPGTYTVTLAVGEEKFTQKLNVLKDPHTSGSESDIQAQTRVQTALYDETN
                     ALAGTVNQIESLRVQIAGFEKELGTDEASKAIRKAADDLAEKLVGVEGTLLQLKLTGR
                     GQDDCRWAPMLLHKIAYLSNQLDGTADFPPTTQQAAVQEELKQRGDKAAQEFHQVVAK
                     DLAAFNAMLREKNISTIFLKTQ"
BASE COUNT         1238 a         1869 c         1847 g         1334 t
ORIGIN      
        1 ttactttcgg gtccacagat tgacccaggg ttcgccggag ttctcgttat acgtgtggtc
       61 aatctagctc caaagcccgt agttttacca taccaagacc ccttcctcac ggttcaattc
      121 ttccgtctta gtgcaccagt cgtcgctcct tattctggcc ctcgacaggg acagactggg
      181 atcagcggcc gagatttgca agagcttgtg gccacggaag gcttgacgct gggccaagtt
      241 ataaaaacct tgaccgcttt agccaaagac gttgcagaat tgcggggcag catcggtcgc
      301 ttgtcgtggt cggttccact tatcgttgcc gttggcatgg ccattgtcgg gatcattgcg
      361 gccatcaaat agccagcaac tcttctgacc cccagacccg ctcagtgagc cccgcttcca
      421 tcgcaggagt aacgcgcaga gtctggtgaa tccggcagaa gttgtaccaa gcgaagtgca
      481 aagcgcaagc cgctttcaga ttttccagct tctttgaaaa ggcgtttgtc aggcgcgtga
      541 accgacgcaa ctgcatccga atcgtaaggt tctgcctctc gatgtgcgac gtggaaatca
      601 agtacggctt ggggtttccg ctgactggta tcggcagagc gtttacgatc tcgccggggc
      661 tgtaccgttt gtccgctaac ccggactctg agtacatctt gattagcatt gcgtagtcca
      721 cgttcgcgcc gaaagcgtcc tcgaccgcag gcaggtaggg ccggaaaccg tctgtggtga
      781 gttggactcg attggcaaga cgaccctgca agtcctgaac gaaataccat gtgttctccg
      841 cgttccgctt cccgacgcgg aagcacggga cgagtttcgt ctctgcgtcc attgccacga
      901 acacgtactg gtcgcctact tcggcaggat tgtcgccctg caccatgcgc ctttcctttt
      961 tgccaacgta agtccagatt tcatcggcct gaacaaaccg gcacggcaat tggcgcatac
     1021 gctggttgag gatttgagcg cagtttgccc ctacccggag taggacgcgc atcaccgtct
     1081 tgtgttccac gtccaccatc cgcgccgtgg cccggatgga attgccctca accagcgcgt
     1141 tgataatctg gatttgcttc tctttcggta gcagattcat cggcttgcct tttctgcgga
     1201 acaagccgat aatccgtttt agggttcggc atgcggctta ctccgcgtgc tggatttggc
     1261 cccgttctcg gttacagccg ggagcggggc ttttgttctc ccgccaaaac tactccctgc
     1321 aagcatggat gtcaagaaaa atagtcatgc atgcagaaat aatttgactt cttcttgcaa
     1381 ggggtatata ggaagcatag ggaggaactg tcagatgggc gaccaaacga tccagtcgaa
     1441 gggcggagac gcccgcgctg cgaaattgac ggctgctgag cgcaaacaga ttgcacgcaa
     1501 ggcagccgaa tctaggtggg gagccgatct gccgcaggct agtcacgacg gccctctgca
     1561 catcggagat gccgtcctga tagccgccaa tctaccgaat gggaaaaggc tgctcagtca
     1621 gacgactatg ctgaccgcca tcggacgatc tcccaatccg cctgctggaa caggcgttct
     1681 agccactctc gacggactcc cctcttttct acgcgcagaa gtgcttaaac ctttcatttc
     1741 agaggagttg atgctctcga cgacccccat ccactttaag ctgaaaaacg gcacccgaac
     1801 ggttgggtac gacgcgatgc ttctgccgat ggtgtgtgag gtttatctga ggctgcgcga
     1861 tcacctctcc agccgactcg ccgatgggga cgcagcggag aaacgtagag caagatcagt
     1921 tctgcgccag tacaaacata tcgtcgaagc gtgcgaccga ctcacacgcg gccttgcgcg
     1981 tcgaggaatc atcgcgctaa ttgacgacgc caccggatat caagcggaca aggcgcgaga
     2041 ggaagtacaa cgcataattc aggcgtatgt tgcgccagcc cttgttccgt ggacgcggaa
     2101 attcccgcat gagtttttcc gcgaagtcta ccgattgcta gggtgggaat acaggccggg
     2161 tcagatcaaa catccccagt acatgggaag attcatcaac aagtacgtct acgaagctct
     2221 tcctccgggc gtgttagacg agttaaaacg cagactcccg aagaacgagc atggaaatcg
     2281 gcgggcaaag ctatggcagt tgctgaccgt tgatactggt agccctcacc tggacaagca
     2341 actgactgcg gacatcacct taatgcaagt ctctgactct aaggcgcaat ttgaggataa
     2401 ctttgagaag gtattcggca tgcagccgcg ccttcctttc ggtacgccga cgaaggagtt
     2461 gaaggggtga gcggctggtg gccctttttg gctaggccgc caccaactct caaaaccaaa
     2521 tgacaccagt acccgcgcgg cgttctgctt gacacacgag cggtgcgagt ataggttcgt
     2581 cgcaccctgg ccggaaccca ggcgcaggtg ggtagagttt ccggctggtc gaggaggatg
     2641 cgatgtcgaa gcggagtgta ggcctggttg tgattgtatt gctcgtcttc agcgcgccgg
     2701 tagctctgtc tcagatggag caggcgcatc ccgtttatac cttcgtgagc caattccagg
     2761 tgccgcgcgc ccagtgggcg cagtatgccg aggacacgga gaaaaatgcc aatccgattt
     2821 tcgaacgctt gatggccgat ggcaccatcg ttggctgggg gaactttgaa cagatcgtcc
     2881 acaccccgga cggcatgacc cacggcgcgt ggtggtcctc cacgagtctc gcaggcatca
     2941 cgcgcgtgct cgatgaaatt cgcaaaggcg gcccgcgccc cggccagatt gcggcaacca
     3001 agcatgaaga tttcctgatg cgctcggtgt attcccacac cagcgctgtc tccggtggag
     3061 gcgccggcta cctgcgggtc aactgcaccc tgacccagcc tggcaagagc gacgatttcg
     3121 ccgccaccat caagaaacat ctgggtccga cgtttgaaga ccagttcaag aaaggcatcg
     3181 ccacctccta tgggatggac gagcaattca tcatcactga gccgggttcg atgcgctgca
     3241 ccgttgtcac gttctccaac gccgagaaca tgaacaagtg ggccgaagcg atcgccgcga
     3301 cactcgacaa gatgagcccc gaagagcgca aaggctggca ggaaggcatt ctcaatacaa
     3361 ccgtgcccga cagccgtcgt gatatcttgg cgcgcatcac gcattctgcc cataagtgac
     3421 gacccgacga acggtcgtta tcctgagaga gcgcagcgag tcgcaggatc tctcttatcg
     3481 acccgctatg gcgcggtcta cgcgtcccgg ggcgggtggc gctgcgaaag aaggcgccct
     3541 cggagagaac aagaaatcaa agtcaaagcg ggacgaaaat cccgccctgg ttcgactacg
     3601 ctcaccacag gtctgaagag agcggcagta acgattccgc gacaggtgtt gaaaaactcg
     3661 aatgcgtgtc attccgagga gtcccggctt tgccgggacg acgaggaatc tgcttttccc
     3721 ctcgccaagg atcaacaata cagatccctc gtcccgtccc gatccttcgg gacgggacga
     3781 gggatgacct gcgcaaagtt tctcagccct cagccaatcc caatatcact gcgtcttcag
     3841 aaaaatggtc gaaatattct tctcccgcag catggcatta aacgccgcca ggtctttcgc
     3901 caccacctgg tgaaactcct gcgccgcttt gtcgccccgc tgtttcaact cttcctgcac
     3961 cgcggcctgc tgcgtggtcg gcgggaaatc cgcggtgccg tcgagctggt tggacaggta
     4021 ggcgatcttg tgcaggagca tcggcgccca tcggcagtca tcctggccgc ggccggtgag
     4081 cttgagttgc agcagggttc cctcgacacc gacaagtttc tcggccaggt cgtccgccgc
     4141 cttgcggatc gccttcgagg cctcgtcggt tcccagttcc ttttcgaacc ctgcaatctg
     4201 cacgcgcagc gactcgatct ggttcaccgt gccggccagt gcattcgtct cgtcgtagag
     4261 agcggtctgc acccgcgtct gcgcctggat gtcgctctcc gagccgctgg tgtgcggatc
     4321 cttcagcaca ttgagcttct gcgtgaattt ttcttcgccc acggccagcg tgaccgtata
     4381 cgttcccggc agcgccagca ccgcgaggcg gccaatggcc ggaagtttgc gccagccttc
     4441 cggtccgaag ggaacatccg gggcgtagag cggtgtggtg cgcagccgaa tttccgtgct
     4501 gcgctcgctt cgcaggtccc accacacgcg gttgattccc gccttgactt cgcaagggcg
     4561 aggctcggca tcgccatctt cgtcttcgct gggggctttg gcctctgcgg cgccgggttt
     4621 tggcgcgcgg cattcgatct cgcggacggt tttaccgccg gcgtcggcga tggtcagctt
     4681 ggcgagatcc ttctctccga ggttggattt gagataaaag ttgattgacg cgccgtaggg
     4741 cggatttttt ccggcggtgg gatcatactc caccgcaccc ggctgcgaga tctccttaaa
     4801 gcggtaggca tcgcgcggcg caaacagatg cgccttggac gcggtcacct ccgacgccat
     4861 ctgctccagc ggcgtaatat cgtccagaat ccagaaaccg cgcccatagg tggccaccac
     4921 cagatcgtgg aagcgctcct gcaccgtcag ccagtaaacc ggcgcgtgcg gcaacccggc
     4981 ctgcagcggc tgccagtttt tcccatcgtc gaaggaaatg tagagcccgc cctcggtccc
     5041 gagataaagc attcccttgc gcgcgggatc ttcgcgcacg cagtgcgcat agctgagcat
     5101 gttgtgcgga actccagcgg tgatcgccgt ccacgatttt ccgtaatccg cggtcttgta
     5161 aacgaacggg tcgcgattgt tcacctgatg tccgtcgacg gtcaggtagg ccgtgcccgc
     5221 gtcgtagcgc gaggcttcga tgttgtccac cgtgccccac tccgggaagt tgggaagatt
     5281 cttggtgacg ttgctccagt tctttccgcc gtcgcgcgtc acctgcacct ggccgtcgtt
     5341 tgtccccgcc cagatcaacc cggcctcctt gggcgactcg gtgagcgcga aaatcacccc
     5401 cgcgtattcc acgccgatgt tgtcaggagt cagcccgccg gaaatctgct ggcggctctt
     5461 gtcgttgcgc gtcaggtcgg gactgatgat ctgccagcta tttccgccgt cggtggtgac
     5521 gtggacatac tggctgccca cataaatctt gttgtggtcg tggggagaaa tggcgatggg
     5581 gaattcccag ttaaagcggt acttcacctc cgcggccggg gcgccggcca cataatcggg
     5641 ccacacctcg acctcgcgat tctggcgcgt gcgctcgtcg aagcgggtta ccgtcccgcc
     5701 cacgctcccc gtgcccgtgc ccgtggacca gatgatgttg ttgtccaccg gatccggcac
     5761 ggcaaagccg ctctcgcctc cggccacggg atgccacgcg ccgcgcggaa tgctgttgcc
     5821 gaagaaaccg ccgaactgca ggctgttgct ggggccgcgc cacgacgggc cgtcctgccg
     5881 gttgccgtaa acgaagtagg gaatctggtc gtccacggtg acgtgataga tctgggcgat
     5941 gggaagattg acatggttcc aggagcggcc gcgattgacg gaaaggttta tgccgccgtc
     6001 gttgaccacg gccatgcgat ccccgttggt gggatcgatc cacatctcgt gattgtcgcc
     6061 gccggggctg gcgggcattt tggtgagcgt gtgtccgccg tcgagggttc tcgaaaatga
     6121 gctggagaag aaaaatactt cgttctcatt gtcgggcgcg atctcttcgc gagtgtaata
     6181 gtgcgtgcgc ccgcggagct ggcggtcgga gttaaccatc tgccagttgt cgccgccgtc
     6241 gtcggagcgc cagagctggc cgagctggcc ggggctgctg ccctgggg
//
LOCUS       DASYVO010000549         3823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_238548, whole genome shotgun sequence.
ACCESSION   DASYVO010000549 DASYVO010000000
VERSION     DASYVO010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238548"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VGR03_18935"
     CDS             complement(<1..563)
                     /locus_tag="VGR03_18935"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEV1996418.1"
                     /translation="MQLGMIGLGRMGANMVRRLQKNGHTCVVYDRSADSVKQLEGEGA
                     TGATSLDDFVKKLQKPRAIWLMVPAAVVDASLHDLVRKLEAGDTVIDGGNSYYIDDIR
                     RANELSAKGIHYCDVGTSGGVWGLERGYCQMIGGETDVVKRLDPIFKTLAPGRGNVDR
                     TPGRDKVAGTAEEGYLHCGPSGAGHFV"
     gene            complement(607..2322)
                     /locus_tag="VGR03_18940"
     CDS             complement(607..2322)
                     /locus_tag="VGR03_18940"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEV1996419.1"
                     /translation="MSVNKQTYKLPNQLAGAVAASLEDWKNNNKVARLWQKDASLWSG
                     TDESNWLGWLTITEEQLAHVDALKQIAEDVKKARFKHALLLGMGGSSLCPEVLRMTFG
                     KIKGFPELHALDSTDPAQIKAIEAKVDLKSTICIVSSKSGSTLEPNIYKQYFFERVKA
                     KVGEKEAGNRFFAITDPGSKMQQVAEADKFRKIFMGVPSIGGRYSAISNFGMVPAAVM
                     GLDVAKFLKNTEEMVNACGASSAVDSNPGVLLGIILGVAANHGRDKITIVTSPGISDL
                     GAWLEQLIAESTGKIGKGIIPVDRERLAKPAAYGNDRVFAYLRLASKPNKAQDAAVAA
                     LEKEGHPVVRITLSNTYNLGQEFFRWEIATAVAGSILGINAFNQPDVEASKIETKKLT
                     SQYETTGSLPPESPFFEEKGIKLFADEKNTAALKGGAKLADVLKTHLSRLGAGDYFAV
                     LGYITMNPANEKILQGIRHAVRDKKKVATVLGFGPRFLHSTGQAYKGGPNSGVFLQIT
                     CDDAKDLPVPGQKYTFGIVKAAQARGDFAVLAERGRRALRVHLGKNLKSGLATLAKGV
                     QQAIA"
     gene            complement(2319..3488)
                     /gene="tal"
                     /locus_tag="VGR03_18945"
     CDS             complement(2319..3488)
                     /gene="tal"
                     /locus_tag="VGR03_18945"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEV1996420.1"
                     /translation="MTTTTTIPSVGTGQGTNPLKALLTYGQSPWMDYIRRDLLTGGGL
                     KIYIDNDGLRGMTSNPAIFEKAITGSNLYNDVLTSPDAKKLDANGVFEKIAIRDVQDA
                     CDIFKPVYAETKRRDGYVSLEVSPFLGFDTKASLAEARRLWKAVDRPNVMIKIPGTPE
                     GLPAIRQALEEGININITLLFAQSAYEQVAEAFLAALEARAAKGQDISHIASVASFFV
                     SRIDSLVDGKIDEKLKAGTDAGQTALLESIRGKIAIANARLTYKKYQELFGGPRWKAL
                     ASKGAQTQRLLWASTSTKNPKYRDVIYVEELIGADTVDTIPPATFDAFREHGKLRPSL
                     TENVDGAAKTMADLGKAGISMKEVTDKLLVDGVKLFADAFTTLLAATGKSAGVRA"
     gene            complement(3582..>3823)
                     /locus_tag="VGR03_18950"
     CDS             complement(3582..>3823)
                     /locus_tag="VGR03_18950"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV1996421.1"
                     /translation="NKLPGIRAAVCHDSYSAHQGVEHDDMNVLVLGSRIIGSKLAEDL
                     VKAFLGAKFTNEERHVRRLNKVKALEAKFAASSKY"
BASE COUNT          715 a         1065 c         1129 g          914 t
ORIGIN      
        1 ttgacgaagt ggccggcgcc ggaagggccg cagtgaagat agccttcctc ggcggtgcca
       61 gctaccttgt cgcggccggg cgtgcgatcg acgttgccgc ggccgggagc gagcgtcttg
      121 aaaattggat cgagacgctt cacgacgtcg gtctcgccgc cgatcatctg gcaatagccg
      181 cgttcgaggc cccatacgcc gccgctggtt ccgacgtcgc aataatggat tcccttggcg
      241 gagagttcat tcgcgcggcg gatgtcgtcg atgtagtagg aatttccacc atcgatcacc
      301 gtgtcgccgg cttcgagttt gcgcactaaa tcgtgcagcg aggcatcgac caccgcggca
      361 ggaaccatga gccagatcgc gcggggtttc tggagtttct tgacgaaatc atcgagggaa
      421 gttgcaccgg ttgctccttc gccctcgagt tgcttcacgg agtcggcgct gcggtcatag
      481 acgacgcaag tgtgaccgtt tttttgcagg cgccgcacca tgttggcgcc cattcttccc
      541 aatccgatca tccccagttg catcgtctgc gctcctcgtt ctatgtgaaa gaaatggttc
      601 agtgatttat gcgattgctt gttgtactcc cttggccagt gttgcgagtc ctgatttcag
      661 attttttccc aagtggacgc gcaaggcgcg gcggccgcgt tcggcgagaa ccgcgaagtc
      721 gccgcgggct tgggcggctt tcacgatccc gaacgtgtat ttctgcccgg gcaccggcag
      781 atcctttgca tcgtcgcagg taatctgcag aaaaacgccg ctattgggac cacctttata
      841 ggcttgaccc gtggagtgca ggaagcgcgg gccgaagccg agaacggtgg ccactttttt
      901 cttgtcgcgc acggcgtgcc ggatgccctg caaaatcttt tcattcgcgg gattcatcgt
      961 gatgtagccg aggacggcaa aatagtcgcc cgctccgagg cgcgagagat gcgttttcaa
     1021 aacgtcggca agcttcgcgc cgcccttcaa ggcagccgta ttcttttcgt cagcgaaaag
     1081 cttgatgcct ttctcctcga agaagggcga ctcgggtggg agacttccgg tcgtttcgta
     1141 ctgactcgtc agttttttgg tttcgatctt gctcgcttcg acgtccggct ggttgaaggc
     1201 gttgatgccg aggatggagc ctgcgacggc agtagcgatt tcccagcgga aaaattcctg
     1261 gccgagattg taagtattag agagcgtgat gcgcacgacg gggtgcccct ctttttcgag
     1321 cgcggcgact gcggcatcct gcgccttgtt cggcttcgat gccaggcgca ggtaagcgaa
     1381 gacccggtcg ttgccatagg cagctggctt ggcgaggcgt tcgcgatcca cgggaataat
     1441 gcccttgcca attttcccgg tggattcggc aatcaactgt tcgagccaag cgccgagatc
     1501 ggagattccc ggcgaagtga cgatcgtaat cttgtcccgg ccatgattgg ctgcgacgcc
     1561 caggatgatt cccaggagga cacctggatt ggaatcgacg gcagaggatg cgccacacgc
     1621 attgaccatc tcttccgtgt ttttcaggaa ttttgcaacg tccaagccca tgacggcggc
     1681 gggtaccatg ccaaagttgg agatcgcaga atagcggccc ccaatgctcg gcacacccat
     1741 gaaaatcttg cgaaacttat ccgcttcggc gacctgctgc atcttggagc ccggatcggt
     1801 gatggcaaag aagcgattgc cggcttcttt ttcgcctacc ttggccttca cgcgctcgaa
     1861 aaagtattgt ttgtagatgt tgggctcgag cgtgctgccc gacttgctcg aaacgatgca
     1921 gatggtgctc ttcaggtcca ctttcgcctc aatggcctta atttgtgcgg ggtcggtgga
     1981 atcgagagcg tgcagctcgg gaaatccttt gatctttcca aaggtcatgc gcagaacttc
     2041 ggggcagagg ctggatccgc ccatgcccag taaaagcgca tgtttgaagc gcgccttctt
     2101 cacgtcctcg gcaatctgct tcagcgcgtc aacgtgggcg agctgttctt cggtgatggt
     2161 gagccagccg agccaattac tctcgtccgt cccgctccag agcgaagcat ccttctgcca
     2221 gaggcgtgcg actttattgt tgtttttcca gtcctcgagg gacgcggcca ccgcgcccgc
     2281 aagctgattc ggcaatttgt aagtttgttt gttgacactc acgcgcgaac cccggcgctt
     2341 tttcccgtcg cggccaacag cgtcgtgaag gcatcggcaa agagcttgac gccgtccacc
     2401 aaaagcttgt cggtcacttc cttcatcgat atgcccgcct tcccgagatc ggccatggtc
     2461 ttcgcggctc cgtcgacgtt ttcggtaagg ctggggcgca gcttgccgtg ttcgcggaaa
     2521 gcatcaaagg ttgccggagg aatggtgtcg acagtgtccg caccgatgag ttcctctacg
     2581 taaatcacat cgcgatactt tggattcttg gtgctggtgc tggcccagag cagacgctgg
     2641 gtttgcgcgc ccttggacgc cagggccttc caacgcgggc cgccgaacag ctcctgatac
     2701 ttcttgtagg tgagccgggc gttggcaatg gcaatcttgc cgcgaatgct ttcgagcaaa
     2761 gcggtttggc cggcgtccgt cccagccttg agtttttcat cgattttgcc gtcaacgagt
     2821 gaatcaatac ggctaacgaa aaagctggca acgctggcaa tgtggctgat gtcctgaccc
     2881 ttggcggcgc gagcttcgag cgcggcaagg aacgcttcgg cgacttgttc gtaggcagac
     2941 tgcgcgaaca ggagggtgat attgatattg atcccttctt cgagagcctg gcgtatggca
     3001 gggaggcctt cgggtgtgcc gggaatcttg atcatgacat tcgggcgatc cacggccttc
     3061 cacaggcggc gagcctccgc gagtgaggct ttggtatcga agccaagaaa tggtgaaact
     3121 tccaggctga cgtaaccgtc gcgccgcttt gtttccgcat acaccggctt aaagatgtcg
     3181 caagcgtcct gcacgtcgcg aattgcgatt ttttcgaaga cgccgttggc gtcgagcttt
     3241 ttcgcgtccg gcgacgtgag gacatcgttg tatagattgc tgccggtgat ggctttctcg
     3301 aaaatcgccg ggttggaggt catcccgcgg aggccgtcat tatcaatata tattttcaat
     3361 ccgccgccgg tcagcaagtc tctgcggatg taatccatcc agggagattg cccgtaggtg
     3421 agcagcgctt tcagcggatt ggttccctgg ccggtgccta cgcttgggat ggtcgttgta
     3481 gtcgtcatac tggttctcct tcacatcgaa aaagcttcga aactctattg tacgccaggg
     3541 agcgtgtggg gcgctcttgc tacgagtctt gctaacttct tttagtattt agaactagcc
     3601 gcgaactttg cttccagcgc ctttactttg ttgagccgcc gcacgtgccg ctcttcgttc
     3661 gtaaactttg cgcccaggaa cgccttcacc aaatcctcag cgagcttcga gccgatgatg
     3721 cgcgaaccaa gcacgagcac gttcatgtcg tcgtgctcca cgccctgatg cgcggaatag
     3781 gaatcgtggc agactgctgc gcgaatcccc ggcaatttgt ttg
//
LOCUS       DASYVO010000550         1584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_194683, whole genome shotgun sequence.
ACCESSION   DASYVO010000550 DASYVO010000000
VERSION     DASYVO010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194683"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VGR03_18955"
     CDS             <1..891
                     /locus_tag="VGR03_18955"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1996422.1"
                     /translation="RRTVGVLVMEHIAVGLVEGGKVTGPLNIYPEKSLTLDPLQSMPA
                     ESIVDCIRQQVERVADGEKIAAIGIGFPGIIRAGVVEDSPNLQQVKGFALQAALSSSL
                     GGKAAGVPVRLFNDADVMAAGIAATQGKLNQLTRVWTLGNGVGFGRYPSSEGIWEGGH
                     AVVTLDPKENFCGCGGRGHLEGILGRRSMRMRFLDLEPEEVFENARAGDARCAEFVML
                     CHRALAAATATNIHMEGPGTFFITGPDVKFIDIALLDRLLHEMVKMSPLQGSRFELVP
                     PSDEMGIIGAAVNAERATSS"
     gene            complement(899..>1584)
                     /locus_tag="VGR03_18960"
     CDS             complement(899..>1584)
                     /locus_tag="VGR03_18960"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV1996423.1"
                     /translation="THEQVLELVQREFGHMNPTGPAESNAAPLPGAPIHLETKKDLEQ
                     VHLCIGVPSVPLGHEDRFGVAVLNNLLGGGMSSRLFQNIREKQGLAYAVFSELTPYSD
                     AGMMTVYAGTAAETVGQVIDLTIQEFRALKESPVTEEELRRSKNHLKGSLMLSLESTS
                     SRMSNLARQELYFGRFYSLDEILAGIEAVTREELQSLSQRYFQAGQIAVTVLGPLNGF
                     TLDRSRLAC"
BASE COUNT          317 a          413 c          545 g          309 t
ORIGIN      
        1 cgcaggacag ttggtgtact ggtcatggaa catatcgccg ttggcctggt ggaaggcggc
       61 aaggtcaccg gcccgctgaa catttatccg gaaaaatcgc taacgctgga cccgctgcaa
      121 tcgatgcccg cggagagcat cgtggattgc atccggcaac aagtcgagag agttgcagac
      181 ggagaaaaga tcgccgcgat cggaatcggc tttccgggca tcatccgcgc tggggtcgtg
      241 gaggattctc cgaacctgca gcaggtgaag ggatttgcat tgcaagcggc attgtcatcc
      301 tccctgggtg gcaaggccgc gggagttccg gtccggttgt tcaatgacgc ggacgtgatg
      361 gccgcgggaa ttgcggcgac gcaaggaaaa ttgaaccaac ttacacgtgt gtggacgctg
      421 gggaatggag tgggtttcgg gcgctatcct tcgagcgaag gaatctggga gggtggacac
      481 gccgtcgtca cactggatcc caaggaaaat ttctgtggat gcggcgggcg cgggcacctg
      541 gaaggcattc tcgggcgccg ctccatgcgg atgcgcttcc tggatctgga gcccgaggaa
      601 gtgttcgaaa atgctcgcgc gggtgacgca cgctgcgccg aattcgtgat gctttgtcac
      661 cgcgctttgg cggctgccac ggccaccaac atccacatgg agggcccggg aacattcttc
      721 atcaccggtc cggacgtgaa gttcatcgat attgcattgc tggacaggct gctgcacgaa
      781 atggttaaga tgagcccctt gcagggcagc cggttcgaac tggtaccgcc atcggacgag
      841 atggggatca tcggcgccgc cgtcaatgcc gaacgcgcca cgtcgagcta ggctggcgct
      901 aacacgccag acgggagcgg tcgagggtga agccgttgag ggggccgagg acggtgacgg
      961 cgatttggcc ggcctggaaa taacgctggg agagggactg gagttcttcg cgtgtgacgg
     1021 cttcgatccc agcgaggatt tcgtcgagcg aatagaagcg gccgaaataa agttcctggc
     1081 gggcgagatt tgacatgcgg gagctggtgg actcgagcga gagcatcagc gagcccttga
     1141 ggtgattctt cgagcggcga agctcttctt ccgtgactgg cgattccttc aaggcacgaa
     1201 actcctgaat ggtgagatcg atgacctggc caaccgtctc cgcagcggtg cccgcgtaca
     1261 cggtcatcat tccggcgtcg gaatagggcg tcagctcgct gaagactgcg taggcgaggc
     1321 cctgcttttc gcgaatgttc tggaagagac gcgaggacat gccgccgccg aggagattat
     1381 tgaggacggc gacgccgaaa cggtcttcgt ggcccagcgg aaccgacggg acgccaatgc
     1441 agagatgcac ctgttcgagg tctttcttgg tttcgagatg gatgggggcg cccgggagcg
     1501 gcgcggcgtt gctttcggcg ggacctgttg ggttcatgtg gccgaactct cgctgaacca
     1561 attccagcac ttgttcgtga gtga
//
LOCUS       DASYVO010000551         2980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_194748, whole genome shotgun sequence.
ACCESSION   DASYVO010000551 DASYVO010000000
VERSION     DASYVO010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194748"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VGR03_18965"
     CDS             complement(<1..762)
                     /locus_tag="VGR03_18965"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV1996424.1"
                     /translation="MLKKKTLTKDRLIWMYTQMVRIREFEERVKRTFVEHPGVIRGHT
                     HLGDGAEATIVGTLATREKNDLAMPSYRCHGYPLVLGTSPRKIMAEIYGRKDGLCGGF
                     GGSMHLADPEHHFPGTSGIIGQAIAHATGAALTAQVKDTGQVIHCFFGDGSTKQGAFY
                     ESLNLAAVWKLPIVYILENNHYQAYTHISLEDANYMAGDPLSQKAQAFSIPGVTVDGT
                     DPLKVYAAVEKAADRARSGKGPSLIEAKFYRLSAHG"
     gene            complement(781..1107)
                     /locus_tag="VGR03_18970"
     CDS             complement(781..1107)
                     /locus_tag="VGR03_18970"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HEV1996425.1"
                     /translation="MSLNEEVEKQEDHRSRFRRAIIVTILRVPKSGGIPTTKARVVRW
                     FKKEGSKIKQGEPVVELETDKVNYELDSPAAGVLLKIVAAVDTEVPVGDPLGCIGQPG
                     ETIPEF"
     gene            complement(1104..2672)
                     /locus_tag="VGR03_18975"
     CDS             complement(1104..2672)
                     /locus_tag="VGR03_18975"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV1996426.1"
                     /translation="MRAIDYFDKGAEAFPDRIAIIDRATHYSYREAREASQRIAQAMW
                     SSGLRGEERVAIISPNDARILLCMLGLMRAGGVWVPINSRNALDANVQYLNYAGTSWL
                     FYHSSFQEQVREIKTLVPTLRHFICIDAENDGDLSLETFMKQGKGGEEIEWGDATGNL
                     NRLVGLVPTGGTTGPAKGVNVTSLSWGTMTELANQCLRCDDIEPVCLSTAPLSHAAGV
                     VAFAMFSLGATNVVLPAFDALEVLQSIERYRVTHIFLPPTAYYALLAHPKLGDFNYSS
                     LRRLLIAAAPVSPYKFKQGLEVFGPCLCQSYGQTEAPMYMTYLDPATVAAAAAGDHPE
                     RLRSCGKPTLAVRLAIMDDDGKILPPLKTGEIVARGALVGAGYYNLPEATAEIRTHGW
                     HHTGDVGYRDEDGFFYIVDRKKDMIITGGFNVYCAEVESCIMELSQVRECAVIGVPDE
                     KWGEAVKAIVVLRDGESLTEEAIIAHCKMRLGGVKSPKSVEFWREIPKTPAGKTDKKT
                     IRKPFWAAADRSVH"
     gene            complement(2686..>2980)
                     /locus_tag="VGR03_18980"
     CDS             complement(2686..>2980)
                     /locus_tag="VGR03_18980"
                     /inference="COORDINATES: protein motif:HMM:NF039925.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5938 domain-containing protein"
                     /protein_id="HEV1996427.1"
                     /translation="AEMANSVQAGTPPRENTRVHRTIDVVLGRGSMNSVQCTMFGSCA
                     YRQTGLVQAFAAHHLALAAPRKVGFASPCEAFGYRELLQALENFGLSKAKLSS"
BASE COUNT          581 a          909 c          834 g          656 t
ORIGIN      
        1 gccgtgcgcg ctcaagcgat agaacttcgc ttcaatcagg cttggccctt ttccgcttcg
       61 ggcgcggtca gcggccttct ccactgccgc ataaactttg agcggatcgg tgccatccac
      121 ggtcacgccg ggaatgctga aggcctgcgc tttttgtgag agcgggtctc cggccatgta
      181 gttggcatct tcgaggctga tgtgcgtgta ggcctggtaa tggttgttct ccaggatgta
      241 aacgataggc agcttccaca cggccgccag gttcaaagat tcgtaaaagg ctccctgctt
      301 cgtcgaaccg tcgccgaaga agcagtgaat gacctggccg gtatccttca cttgcgcggt
      361 cagcgccgcg ccggttgcgt gcgcaatggc ctgcccgata attcccgatg tgccgggaaa
      421 atgatgctcc gggtcagcca ggtgcatcga gccgccgaag ccgccgcaca gtccgtcctt
      481 gcggccgtaa atctccgcca tgatctttcg cggggaggtg ccgagcacga gcgggtagcc
      541 gtggcagcgg taactcggca tcgccagatc gttcttctcg cgcgtcgcga gggtgcccac
      601 gatggttgct tccgcgccgt cgccgagatg cgtatgcccg cggattactc ccgggtgttc
      661 gacgaaagtg cgcttgacgc gctcctcaaa ctcccggatg cgcaccattt gcgtatacat
      721 ccagatcagc cggtctttcg tgagagtttt tttctttagc atggttaaaa tctccttgct
      781 tcaaaactcc ggaatagttt ccccgggctg gccaatgcag ccgagcggat cgccaacggg
      841 cacttccgtg tccactgcgg caacgatctt gagcagcaca cccgcggcag gcgaatcgag
      901 ctcgtagttc accttgtcgg tttccagctc cacgaccggc tcaccttgct tgatcttgct
      961 gccttccttc ttgaaccagc gcaccactct tgccttggtc gtcgggattc cacccgactt
     1021 gggaactcgt aaaatcgtaa cgatgatggc cctcctgaat cgcgatcgat gatcttcctg
     1081 tttctccact tcttcgttca aactcaatgc acggaccggt ctgctgccgc ccaaaagggt
     1141 ttacgaatgg tttttttgtc cgttttacct gccggcgtct ttggaatctc gcgccagaat
     1201 tcgacggatt taggcgattt cacaccgcca agccgcattt tgcagtgcgc gatgatcgct
     1261 tcttccgtca gggattcgcc atcgcgaagc acgacgatcg ctttcaccgc ttcgccccat
     1321 ttttcatccg gcacgccaat caccgcgcat tcgcgcactt gcgaaagttc catgatgcac
     1381 gattcgactt ccgcgcagta cacattgaag ccgccggtga taatcatgtc cttcttgcga
     1441 tcgacaatat agaaaaagcc gtcctcgtct cggtatccga cgtcacccgt gtgatgccag
     1501 ccgtgggttc gtatttccgc cgtcgcttcc ggcaaattgt aataaccggc gcctactaga
     1561 gcgccgcgcg cgacgatttc gccggtcttg aggggaggaa ggattttgcc gtcgtcatcc
     1621 ataatcgcga gccgcaccgc aagagtcggt ttgccgcaac ttcgcagccg ctccggatga
     1681 tcgcccgcgg ccgcggccgc caccgtcgcg ggatcgagat acgtcatgta catgggcgct
     1741 tcggtctgcc cgtaggattg gcacaggcac gggccgaaaa cttcaaggcc ttgtttgaat
     1801 ttgtacggcg agacgggcgc cgcggcgatt aagagccgcc gaaggctcga atagttgaaa
     1861 tcgccgagct tgggatgcgc gagcagcgca tagtatgcgg tcgggggcag gaaaatgtgg
     1921 gtgacgcgat aacgctcgat actctgcagg acttccagcg cgtcgaaggc gggaagcacc
     1981 acgttggtgg cgccgagcga gaacatcgca aaggccacga ctcccgcggc gtgagaaaga
     2041 ggcgcggtgc tgaggcagac aggctcgatg tcgtcacaac gcaaacactg attggccagc
     2101 tcggtcatcg tgccccagga caagctcgtc acattcacac ctttcgccgg tccggtggtc
     2161 ccgcctgtcg gcacgaggcc cacaagacga ttcagattcc cggtagcatc gccccattcg
     2221 atctcctctc ctcccttacc ttgcttcatg aaagtttcca gtgagaggtc gccgtcattc
     2281 tccgcatcaa tgcaaatgaa gtggcgcagc gttggcacga gcgtcttaat ttcgcgcacc
     2341 tgttcctgaa agctgctgtg ataaaacagc caggacgtgc ccgcatagtt cagatattgg
     2401 acgtttgcgt ccagcgcgtt acgagaattg atgggaaccc aaacccctcc ggcgcgcatg
     2461 agtcccagca tacaaagtaa aatccgggcg tcgttgggcg aaatgatggc gacgcgctcc
     2521 tcgccacgca atccggagga ccacatcgcc tgggcaattc gctggcttgc ttcgcgtgcc
     2581 tcgcgatagg aatagtgcgt cgcgcgatcg atgatcgcga ttctatcggg aaacgcttcc
     2641 gctcccttgt cgaagtaatc gattgcccgc attccgccgg atcctctagg aactgagctt
     2701 cgccttcgaa aggccaaagt tttcgagcgc ttgcaaaagc tctcggtatc cgaaagcttc
     2761 acacggggaa gcaaatccaa ctttgcgcgg tgcggccagc gcgagatgat gcgccgcaaa
     2821 cgcctgcacc aggcccgtct gccggtacgc gcagcttcca aacatcgtgc actggacgga
     2881 gttcatgctc ccgcgcccca aaaccacatc gatggtgcga tgcactctcg tgttttcccg
     2941 tggaggcgtt cccgcctgca cggaatttgc catttcagca
//
LOCUS       DASYVO010000552         3504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_238925, whole genome shotgun sequence.
ACCESSION   DASYVO010000552 DASYVO010000000
VERSION     DASYVO010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238925"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VGR03_18985"
     CDS             <1..576
                     /locus_tag="VGR03_18985"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1996428.1"
                     /translation="LKRQGVVSSPTENSSHPGQRFRRLRTRRGLSLAQVARATGVSIG
                     FLSALERGQMRSSIATLRRIARFYRTNILSLFETAGDNPRLVRPNQRKILETTPDVRM
                     ELLAWGNTAMEPHLFRIKPGGGSGESYSHEGEEFLHVLRGNFEIWLDAKEHYHLKPGD
                     SLYFESSTPHRWKNPGRTETRLLWINTPPTF"
     gene            596..2314
                     /locus_tag="VGR03_18990"
     CDS             596..2314
                     /locus_tag="VGR03_18990"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV1996429.1"
                     /translation="MKIHPLRVVLQSLPLLLCAASLSRAQGRMVEPADIIVVHARVYT
                     EDLKQPWAQAVAIYRGKIVAVGDDPVIERMRGMGTKVINAGGKLVLPGFVDCHIHFID
                     GSFSLGRVNLEGAKDAVDIQKRLREYASEHPGDDWILGRGWNYAMFGPEALPHKKYLD
                     EIFPNRPVFLEGYDGHTYWANSKALAMAGIVRETPDPPNGAIVRDPKTGEATGALKES
                     AQDLVAKITPKPSRAEKLLALRAGMKWANEHGITRVHSAGGDFEVLDLFDEMRHRGDL
                     SVRMYIAYFLNPPELRSQDLDAIEHARKKFHDDWIDANAVKFMVDGVVESHTAAMLEP
                     YSDDPSLKGKLFWEPSRYKAAVAELDKRGLQLFTHAIGDYAVRTALDAYENAETRNHK
                     RDHRPRIEHIETISAADIPRFGRLGVIASMQPLHSYPDADTLDVWAHNVGPERASRAW
                     AWKSISDAGGRLAFGSDWPVVTLNPWEGVQTAVTRQTSEGQPEAGFVPEQRLTVAQAI
                     DAYTLGAAFAGRREKSEGSLEIGKLADLIILSQNIFDIDPHKISATKVVTTVVGGRLV
                     YQADAK"
     gene            2327..3388
                     /locus_tag="VGR03_18995"
     CDS             2327..3388
                     /locus_tag="VGR03_18995"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1996430.1"
                     /translation="MRSRAILLGILTLALACCACKKKEESLSLLVWEGYSDDSFVRAF
                     EESHHCKVVASYMGSSDELVAKLRGGSASNYDVISPSSDVAASLARTGLAAPLDLSKL
                     PSYAQLSSKLRDLPLVKANGQVYGVPFMWGPNPLLYDTTVFAQPPDSWTVFWDPKYKG
                     KISVWDDLSTVYLAAQILGFDKPDPSQLYNLTDDQLAAVRKKLIELKPNIRKIWATGG
                     ELTNLFQSHEVVLAMGWPLNTNDLRKLNFPIGEVIPKENTTGWIDHLMITAASSHKEL
                     AHAFLEYMIEAKTQKLVTDRTHYTPANPGASQFLTSEEIKGLHLDNPDAYMQRIYFWQ
                     DVPRRAKYNEIWNEVKAAQ"
BASE COUNT          765 a         1093 c          952 g          694 t
ORIGIN      
        1 ctcaagcgcc agggcgttgt atcctctccc acagaaaact cgtcgcaccc agggcagcgc
       61 ttccgccgat tacggacccg ccgcggcctt tccctggctc aagtggcccg cgccaccgga
      121 gtctccatcg gttttctcag cgccctcgag cgcggccaga tgcgctcctc gattgccacg
      181 ctgcgccgca tcgcccgctt ctaccgcacc aacattcttt cactcttcga aaccgccggc
      241 gacaatccgc gcctggtgcg cccgaatcag cgaaaaatcc tggagaccac cccggacgtg
      301 cgcatggaac tgcttgcctg gggaaacacc gcgatggagc cgcatctttt tcgcatcaaa
      361 cccggtggcg gcagcggtga atcctactcg cacgaaggcg aggaattcct gcatgtcctg
      421 cgcggaaatt ttgagatctg gctcgacgcc aaggagcatt atcatctgaa gcccggcgac
      481 agcctctatt ttgagagttc gactccgcac cgctggaaga atcccggccg tacggagact
      541 cggctgctgt ggatcaacac gccgccaaca ttttgaagct taggaccagg tgccgatgaa
      601 aattcacccg cttcgcgtcg ttcttcaatc gctcccgctc ttgctctgcg ctgcctcgct
      661 ctcacgcgcg caaggccgga tggtcgaacc ggcggatatc atcgtcgttc acgcccgcgt
      721 ttacacggaa gacctcaaac agccgtgggc ccaggccgtg gcgatttacc gcggaaaaat
      781 cgtggccgtc ggtgacgatc ccgtaatcga acgcatgcgg ggcatgggca caaaggtgat
      841 caacgccggt ggaaaacttg tgctgccggg tttcgttgat tgtcacatcc attttatcga
      901 cggctcattc agtctcggcc gggtgaatct tgaaggcgca aaagacgccg tcgacattca
      961 aaaacgcttg cgcgaatatg cctccgaaca tcctggagat gactggatcc tcggccgcgg
     1021 ctggaattac gccatgttcg gtcccgaggc gcttccgcac aaaaaatact tggatgagat
     1081 ctttcccaat cgcccggttt ttctcgaagg ctatgacggg catacctact gggccaattc
     1141 aaaggctctc gcgatggcag ggatcgttcg tgaaactccg gatccgccca acggcgcgat
     1201 tgttcgcgac cccaaaacgg gtgaggcgac gggtgcgtta aaagagtcag ctcaagacct
     1261 ggtcgccaag atcactccaa agccgtctcg cgccgaaaag cttttggcgc tgcgcgcggg
     1321 tatgaaatgg gccaatgaac acggcattac tcgcgtacac agcgccggcg gcgattttga
     1381 ggtgctcgat cttttcgacg aaatgcgcca ccgtggcgac ctcagcgtgc gcatgtacat
     1441 cgcttacttc ctcaatccgc ccgagcttcg gtcccaggac ctcgacgcca tcgaacacgc
     1501 ccgcaaaaaa ttccacgacg actggatcga cgctaacgcc gtcaaattca tggtggatgg
     1561 cgtcgtcgaa tcgcacaccg cggcgatgct cgagccctac agcgacgatc cctcattgaa
     1621 aggcaagctc ttctgggagc cttctaggta caaggcggca gtcgcggaat tggacaagcg
     1681 cggtttgcag cttttcacgc atgccatcgg cgactacgcg gttcgaacgg cgctggatgc
     1741 ctatgaaaat gcagaaacgc gaaaccacaa gcgcgatcac cggccgcgca tcgaacacat
     1801 agaaaccatc tccgccgcgg atatcccgcg tttcggcaga ctcggtgtca ttgccagcat
     1861 gcaaccgctg cattcttatc cggacgcgga tacgctcgac gtatgggcgc acaacgtagg
     1921 ccccgaacgc gcttcgcggg cctgggcatg gaagagcatc tccgacgcgg gcggccgcct
     1981 ggccttcggc agcgactggc ccgtcgtgac tctcaatccg tgggaaggcg ttcagaccgc
     2041 tgtgacgcgc caaacgtccg aaggtcaacc cgaagcgggg tttgtccccg aacagcgatt
     2101 gaccgtggcg caagcaattg acgcttatac gctcggagcg gcgtttgccg gccgccgtga
     2161 aaaatcggaa ggttcgctgg agatcggcaa gctcgccgac ctgatcatcc tttcgcagaa
     2221 tatcttcgat atcgatccgc ataaaatttc ggccacaaag gttgtcacca ccgttgtggg
     2281 cgggcggctg gtctatcaag cggatgcaaa gtaaccggga ggagcaatga gatctcgcgc
     2341 cattcttctg ggaattctga cgctcgcatt ggcctgctgc gcctgcaaga aaaaagagga
     2401 atcgctaagc ctgctggttt gggaaggtta ttccgacgac tccttcgtgc gagccttcga
     2461 agaaagccat cactgcaaag tcgttgcatc ctacatgggc tccagtgatg aactggtcgc
     2521 gaaacttcgg ggcggcagcg cttcgaatta cgacgtaatc tctccctcca gcgacgtcgc
     2581 cgcctccctt gcgcgtaccg gcctcgccgc gcctctcgat ctttcgaaat tgccgtccta
     2641 cgcgcagctt tcctccaaac tccgggatct tccgcttgtc aaagccaacg gccaggtcta
     2701 cggcgtcccg ttcatgtggg gaccgaatcc cctcctctat gacaccactg tctttgcgca
     2761 gcccccggat tcttggacgg ttttttggga cccgaaatac aaaggcaaaa tttccgtctg
     2821 ggatgatctt tccaccgtct acctcgccgc tcagatcctc ggcttcgaca agccggaccc
     2881 ctcgcaactt tacaatctta cggacgatca gctcgccgcc gtcagaaaaa aactgatcga
     2941 actcaagccc aacattcgga agatctgggc cacgggtgga gaactaacca atctctttca
     3001 gagtcacgaa gtggtgctgg ccatgggctg gccgctgaac accaacgacc ttcgtaaatt
     3061 gaatttcccc atcggcgaag tgattccgaa agaaaacacg accggatgga tcgaccacct
     3121 gatgatcacc gccgcgagca gccacaagga attggcgcac gcttttctcg agtacatgat
     3181 cgaggcgaaa acccagaaac tggtcaccga tagaacgcac tacactccgg cgaatcccgg
     3241 ggcgtcacag tttctgactt ccgaggaaat caaaggcttg cacctcgata accccgatgc
     3301 ctacatgcag cggatctatt tctggcagga cgttccgcgc cgcgcgaagt acaacgaaat
     3361 ctggaacgag gtcaaagccg ctcagtgagc tccgccgcag ccaacccgca gcaccatctc
     3421 tcggccgcgc cgggaacact cctcaaagtg cgcggagtgg ccaagcgctt cggcacgcgg
     3481 gatgtgctga aggacatctg tttg
//
LOCUS       DASYVO010000553        29157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_224211, whole genome shotgun sequence.
ACCESSION   DASYVO010000553 DASYVO010000000
VERSION     DASYVO010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 29157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29157
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224211"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VGR03_19000"
     CDS             complement(<1..316)
                     /locus_tag="VGR03_19000"
                     /inference="COORDINATES: protein motif:HMM:NF014809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HEV1996431.1"
                     /translation="MLRRRRSAGSTAPALIFSLLLVFLVGITVYLFAAKTWWFPKSIT
                     AFGVEVDKQFHRTLVITGIVFVLSQLGLAYAVFKFRDRGQRASSSHGNTSMEVLWTLA
                     TVV"
     gene            737..1894
                     /locus_tag="VGR03_19005"
     CDS             737..1894
                     /locus_tag="VGR03_19005"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1996432.1"
                     /translation="MKISSSLFRVTLALGMVISLPAGMAAQKGGKGGPPGKPGANSSP
                     INSPQPTPTQPLLISGKVLVDGSGAPPEPVLIERVCNGVARRQGYTDSRGSFQFQLDQ
                     ISGFQDASENSGIAGNDTQMTTQAQDTLKVKYQGCEIRAVLPGFLSSAVSLRVQGSTW
                     QYDLGTIFLKRMENVTGTTISMTTLKAPGDAKRAYEKARKAFDEKKFLDAEKELDKAV
                     KIYPGFAAAWSLLGDIHQQQKQLELAVKDYGQALTADPQFVNPHFGLALIAMQEKRWQ
                     DAVQFTNQVLKMNPAAFPSAYFYNAVAAFNLGRVDPAEVSARKFKSLDTDHRHPDICL
                     LLGQIFIQKQDYASAAQEMRDYLALVPNASNAQEVREALKRLEDAIVATKH"
     gene            complement(2676..2760)
                     /locus_tag="VGR03_19010"
     tRNA            complement(2676..2760)
                     /locus_tag="VGR03_19010"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2724..2726),aa:Leu,seq:gag)
     gene            complement(2832..3311)
                     /gene="secG"
                     /locus_tag="VGR03_19015"
     CDS             complement(2832..3311)
                     /gene="secG"
                     /locus_tag="VGR03_19015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV1996433.1"
                     /translation="MPTAFKWAIALLIGFGIVALGWRLANSAWFFWVLPAFFIANCLV
                     LIIVVLLQSGKAADLAGAFGGAGSQTAFGPRGAANVLSRATTWCAVMFMVCALALVLQ
                     KERTGSTGSSVLEKVAQPAPKSAPPAQPQSQPAAPLGGAQNPPATQQQPPPPPTNKP"
     gene            complement(3325..4074)
                     /gene="tpiA"
                     /locus_tag="VGR03_19020"
     CDS             complement(3325..4074)
                     /gene="tpiA"
                     /locus_tag="VGR03_19020"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV1996434.1"
                     /translation="MRRRVIAGNWKMYKTPAETRAFFSALAPLVIHSTHCDIVVAPPF
                     PAIPAAVDAASALPIAIAGQDVFWEKEGAFTGEVSAGMLAAAGCRYVIIGHSERRQYF
                     AETDEAVAKKTKAALAAGLIPIVCLGEMLADREAGATEKILARQFAGGPGALTPEEFS
                     RILLAYEPVWAIGTGRTATPEIAAAAHRFLRHCAADRFSAKLATALRILYGGSVKPDN
                     IQGLMAQEELDGALVGGASLVPLSFAAIVNF"
     gene            complement(4071..5267)
                     /locus_tag="VGR03_19025"
     CDS             complement(4071..5267)
                     /locus_tag="VGR03_19025"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV1996435.1"
                     /translation="MNKLTMGDLNLRGNRVFIRVDFNVPLKDGFVADDTRIRETLPTL
                     RLAVQMGGRLVLASHLGRPKGAPDVQFSLRPVARKLEELLGKPVGFSPNCVGLIAETK
                     SKSLTDGEILLLENVRFQPGEEKNDEVLSREFAALCDGLFICDAFGSAHRAHASVVGI
                     TRFVQKAAAGRLMEKELEYLGRALTNPARPYVAILGGAKVSEKIEVVENLMNIADSML
                     VGGSMAYTFLKAQGLPVGKSLVEEDKVELAARLLAEARRRSFPLMLPVDHVVAPEFKA
                     DSPATVCDVAAIPADQMGLDIGPKTVAAYAAEIAKTKMIVWNGPMGVFEMPAFAKGTL
                     GVARAIAAATSSGAISIVGGGDSVAAVHQSGVADQISHISTGGGASLEFLAGRKLPGV
                     EALTNL"
     gene            complement(5294..6298)
                     /gene="gap"
                     /locus_tag="VGR03_19030"
     CDS             complement(5294..6298)
                     /gene="gap"
                     /locus_tag="VGR03_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV1996436.1"
                     /translation="MPIKVGINGFGRIGRNIMRTALDDRDIQFVAVNDITDAKTLAHL
                     LKYDSILGNLPHKVTHTEDSIAVEGKSFKVFKTRDPGEIDWASVGADIVVESTGLFTK
                     GADARRHMRGPVKKVIISAPATDPDHTLVLGVNDKTYDPLKHHVVSNASCTTNCLAPV
                     AKVLLESFGIVSGTMTTIHSYTNDQKLLDLPHKDLRRARAAAVSMIPSSTGAAKALHL
                     VIPELKGKLDGYAMRVPTPNVSVVDLTVFTERPASVESVNAALKAAANGPMKGILDYT
                     EEQLVSADFRGNPHSAIVDAEYTKVVGDRCVKVLAWYDNEWGYSCRCRDLIKFMASKF
                     "
     gene            6590..8938
                     /locus_tag="VGR03_19035"
     CDS             6590..8938
                     /locus_tag="VGR03_19035"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEV1996437.1"
                     /translation="MRTSAKTLGFVVVTGCLAGIIPGASAAGQKQTKPAPTPVFATLH
                     IPSLEQAPKLADFLDMQPSPAFEGKMLKVDKFVQRDPHDGAPVSQRTEAYLGYTDKNF
                     YAVFLAFDSEPQKIRARMLRRELIDDDDQVGMFLDTFHDRRHAYVFYSNAYGIQQDGL
                     FSENQGPDYSWDTVWRTETKMNSRGYMVLFEIPFKSLRFRQQASIDWGVMLARVIPRN
                     SERSYYPVNTGKLQGWLSQEGTLEGFHGISPGRNMQFIPYASVGAFRALDERDPAGVR
                     FTGKHVEPKAGLDSKIVIKDSLVLDATINPDFGQIESDDPQVTVNQRFEVFFPEKRPF
                     FQENSNYFQTPLNLVFTRRIIDPLYGVRLTGKIDRWAIGTFVANDRSPGKSVIDTDPL
                     RGNNALFGVLRVNREVGKDDSIGFIFTDRELHTVPNSFCTTTICAVGFNRVGGFDTQI
                     KINRNWQMNAQAVTSETKFSNGIQKAGPAYQVYLERSSRDLEFNSLYQDISPGFHTDT
                     GFVNRTDFRRFSNFIGYKSHRKGKLLVAHGPSVFEQALWDHNGTRLQYLFNGNYEWDF
                     QRQSYFGFFGNIEHERLRPVDFSSLTDNRDYGHHLVGPVFGTQYFKWLNLNGEIDLGT
                     ATNFVPRAGPPVLANFTAIILRGVVRPIKGLTIENTYLLTRLLDQQTDLNIFNNHIIR
                     SKWNYQFTKEFSLRIIGQYVTTISNPAFTTLPSAKKFNGDVLFSYLLHPGTAVYIGYN
                     SDLQNLDPTLGLDPNGNLLRTRSRFLNDGREIFIKISYLLRY"
     gene            complement(9010..9918)
                     /locus_tag="VGR03_19040"
     CDS             complement(9010..9918)
                     /locus_tag="VGR03_19040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996438.1"
                     /translation="MRGWLTIPLIFLLFADADMPMIYRLRPSPAPAPDFPPGLGIPVL
                     PSVLPVLGNGQSQQQSSARSPGSRLSPQSELALVRYVSGEFAKAVKPLPGGKEGFLLY
                     VGKPLDQKLLDRAVNTHGAAVNTGDNVQITMLYFREHDIVVDVNGGGRVKKRFRDRIH
                     IDFGGLPTVKTTQPQTGPPGLKSGMGSTIFLEFNQPIPDLSPEDLKNLLAPLLDFSKQ
                     RSASVQWYDTLAPEMKKAIQDRHPVLGMDREMVVAAIGRPAHKIRERDSDGNEIEDWI
                     YGQPPSKTVFVRFQGDRVTSIKQFPQ"
     gene            complement(9985..10995)
                     /locus_tag="VGR03_19045"
     CDS             complement(9985..10995)
                     /locus_tag="VGR03_19045"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996439.1"
                     /translation="MRIEARAPTRVDLAGGTLDIWPLYLFHPGAVTVNAAITRYASCV
                     LETHPAGNAKIQLVSRDTKREESFASLSALADAKKPRLPLLAELIKIFQPAGGFTLTT
                     DSEAPAGAGIGGSSALAVAICAALDKCTGAGKSKVEWIHISRDAEAIVIRVPTGTQDH
                     YPPAFGGAAVIELLPGSERRSELRANLDELERRLVLCYTGKPRQSGVNNWEVFKAHIN
                     GNKGIQRNLAKISAIAQEMRHALERSDWNAAGKIMREEWSFRRKNLPTISTRTIDAII
                     GGARSQGALAGKVCGAGGGGCVVLLIEPDSRPAVETAVERAGGQLLPIKIDRLGVQVH
                     AG"
     gene            complement(10992..11621)
                     /locus_tag="VGR03_19050"
     CDS             complement(10992..11621)
                     /locus_tag="VGR03_19050"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003296.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV1996440.1"
                     /translation="MDIVKGNMGWIEVIVGPMFSGKSEELIRRLRRAEIARQRVQIFK
                     PAIDARYAANEIVSHSGLGIASENVTRGEEVLARVTARTEVVGIDEAQFLGEEVVGVC
                     TKLADMGKRVIVAGLDTDYRGRPFEPMPRLLSVAEEITKLLAICVRCGNPAVHTQRLV
                     ESEELIVVGASGMYEARCRRCFEANIAQAEKEPSPLTPSRIRAVGSGNK"
     gene            complement(11668..12261)
                     /locus_tag="VGR03_19055"
     CDS             complement(11668..12261)
                     /locus_tag="VGR03_19055"
                     /inference="COORDINATES: protein motif:HMM:NF014041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HEV1996441.1"
                     /translation="MEKIIVAVGSRRRPKLNAVREALGFIGAALDPGAAFDVAGVEVE
                     SGVSHTPLSREELMRGARRRAERLIQLARSHAEPWRYFVGLEGGLDVLHEDGRRRVFL
                     ESWAYVSDGALGFFGCSGGIEMPEALAREVLEDGVELAAAIDRFAGAAGIRDAQGAWG
                     VLSRDLITRQEAFRVAVVSAFAPFYNAKMYRTAGAAR"
     gene            complement(12367..12440)
                     /locus_tag="VGR03_19060"
     tRNA            complement(12367..12440)
                     /locus_tag="VGR03_19060"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12406..12408),aa:Gly,seq:gcc)
     gene            complement(12554..12751)
                     /locus_tag="VGR03_19065"
     CDS             complement(12554..12751)
                     /locus_tag="VGR03_19065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996442.1"
                     /translation="MKKKRKKASFSSDRRRRHHHWLVTVYYADGEKFGRVYTDRDKAA
                     RFAERQRKSPVVKSARVSQLS"
     gene            13268..13528
                     /locus_tag="VGR03_19070"
     CDS             13268..13528
                     /locus_tag="VGR03_19070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996443.1"
                     /translation="MNILKHPPIVEDLRNHSPEQLAELRMLLEAEAPARPDPRRPRFF
                     EIQGSTSVFYIFKYPSGTKVLLIGVWDRDRVAELVTCACPAA"
     gene            complement(13615..14013)
                     /gene="msrB"
                     /locus_tag="VGR03_19075"
     CDS             complement(13615..14013)
                     /gene="msrB"
                     /locus_tag="VGR03_19075"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1996444.1"
                     /translation="MKDKLSKSEADWKKQLSPNQYYVTRQRGTEPPFSGEHEDTETPG
                     TYKCVCCGQPLFRSGEKYHSGSGWPSFWAPADQNAIETETDASHGMKRTEVKCSRCDA
                     HLGHVFEDGPRPTGLRYCINSAALDLDEEK"
     gene            14279..15343
                     /locus_tag="VGR03_19080"
     CDS             14279..15343
                     /locus_tag="VGR03_19080"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEV1996445.1"
                     /translation="MSLRGLPVRRAVLRGKGEVPELEGRAGKLRLDLNENTVGCSRAV
                     TRALRRLSPQQIAMYPEYRRVTSRLARGEGVRPEEMHLTNGGDDALRIVFDVFVDAGS
                     TVLFPEPTFPMYRFFAGVYGARVIAPRFDREMNFPVAELLAALLRRPRVLFLANPNNP
                     TGNMLPPADLRRILRAARRTVVVVDEAYVEFSGWSAAAWIRRYPNLVVARTYSKAAGL
                     AGLRLGCLIARAEVTELFRRVAPPFNVNAAALIAADAAMRDRGAARRYVAEVRRARIE
                     FEKQLARLGYRSLPSVANFVLVDFGPRGPEIVRRLKRQGILLSDRSRDSRRRGMVRVS
                     IGTRREMRRLAAALARLARK"
     gene            complement(15360..18530)
                     /locus_tag="VGR03_19085"
     CDS             complement(15360..18530)
                     /locus_tag="VGR03_19085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEV1996446.1"
                     /translation="MISRIIDWCASNRFLVFTGTLLLVLAGIWSLKNIPLDALPDISD
                     VQVIVHIPWEGEPPSIIEDQVTYPIVTTLLAAPHVKAVRAQTMFGDAYVFVVFEDGTD
                     LYWARSRVLEYIQQLQGVLPKNVSPMIGPDATGAGWVYEYAVIDRSHQHSLADLRSLQ
                     NWYLRYQLETVPGVAEVASIGGFVRQYQVNLDPNKLRAYNIPLSMVIDRVRESTNEVG
                     GRVLEMGGAQYMIRGLGYLRSLQDLETVPVATKNGTPVLVRDLGTVTFGPDIRQGVVE
                     WNGEGETVGGIIVMRYGMNALNVIDGVKKKLAEIKPSLPPGVEIVSGYDRSGLIQASI
                     ETLQRDLLEEAIIVSFVTIVFLFHFRSALIPILTLPIAVLASFIPMYFLHVSSNIMSL
                     GGLALAIGVLIDAAIVMVENGYRHLSEHIATTGASGAKLTEKERRRILMGAAKQVGPA
                     IFFSLLVILVSFLPVFLLEAQEGRMFRPLAWTKTLSVAFSSILAITLVPVLMVIFIRG
                     NLRPESANPFSRWTQALYLPVLRFCLRYRKTTLFLNLIFLVVTLPLAWRMGSQFMPPL
                     YEGSSLYMPTALPGISITQASALLQEQDRIIKSFPEVESVFGAVGRSDSPTDNAPLDM
                     YDTTIMLKPREQWRAGMTYEKLIREMDEKLKFPGLTNTWTMPVQNRLDMELTGIKTPV
                     GIKIQGPNVEGIQRAGAQLQQVLGGMTELRSIFAERVAEGFYINVEVNRPEAARYGLT
                     VADVQRAVTSGIGGENVAENIEGRERYPINVRYSRDFRDDIEKLRRVIIATPAGAQIP
                     ISEVAKVYFSRGPAMIRDEDGLLTGYVYLDLATKDYGGFVAKANRLLNENLRLLPGYT
                     YKWSGEYEFEVRAKERLKFIVPLVFFVIFLLLYMVFHSATEAAVLIFPTFYAMTGGLI
                     LQYWLGYNFSVAVWVGYIALFGIAVETGVVMVVYLHESLDRRLSSGIPLKHEDIEAAV
                     IEGAVQRLRPKLMTVTAVLASLIPILWESGIGSDVMKPIAAPIVGGMITSTIHVLILV
                     PVFFALMKERALRRGTLQPREPSSNA"
     gene            complement(18533..20095)
                     /locus_tag="VGR03_19090"
     CDS             complement(18533..20095)
                     /locus_tag="VGR03_19090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV1996447.1"
                     /translation="MRNYRKAFFLALIGNLVFIVVLAGIWWNVRRSAGSQHAAVSAPN
                     AAKQPVQSGSLGSAPPPAETPLAPVQLSAERLQSIGVKFGEVARKPVQDEIRTTGTVA
                     IDETRLSYVQTRFSGYIQKVFADATYQYVRKGQPLFTIYSPELAAAEREYLVARQNAQ
                     ALSQSTVPGVAAGIASLVDASADRLRQWNVPQREIARLESSGQVEQGLEIDSPVTGYI
                     TERNALPNLTVQPDTRLYTIADLSTVWVFAHVFQNDLGRIRVGAPTALTVDSYPGRVF
                     RGRVDFIYPEVDATTRTARVRLVFSNPNLTLKPGMYVNVVLQVPLGNQLVIPVSGVLQ
                     SGARQIVFVDRGAGYLEPRDVQLGPQAGDQYVVLKGLKAGERIVTSANFLIDSESQLQ
                     AAIGSFVPPPPGAGAASAMNTPQVNVELSSDPAPPHKGGNVFRVKLTDANGAPISGAE
                     VSVTFFMPAMPAMGMAAMRTPVTLSDKGKGLYEGSGQLESGGTWQVTILAKKNGQLLA
                     TKQLSINAEGGM"
     gene            complement(20119..21480)
                     /locus_tag="VGR03_19095"
     CDS             complement(20119..21480)
                     /locus_tag="VGR03_19095"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV1996448.1"
                     /translation="MSALHGEKSHNEFLVVRILCITVLLVFFAHTPRAAGQEQTGGSA
                     QVASPPQAIPHPGTVTPLQDLLTEAERNNPQIQAARHGWESAQQVPTQVSTLPDPQFV
                     LQHVSVGSPRPFAGYSNSDFAYIGLGVSQDFPYPGKLRLRGEIAKKDADVTQQKYESV
                     RRAILAAVKMAYFQLGYLAKRQMILEGDGQLLQQIEQSAEARYRSGMGNQHDVLQAQL
                     ERTKLLREITTNQLEAGRFQAEMKQLLNRPQSSPDIETVELTESKVAYTYDQWLSAAK
                     ANNPEITGAQKMVERQGLQVNLAKKDFYPDFNVQYMWQRTDPTQFRAYYMLTLGVRIP
                     IYRGRRQQPELAQAEADQSRAKSEHEAQTQQVAFQLRQQFLSSEKSAELLKIYREGLI
                     PQARAELQAGMAGYQSNRQDFQALLTSFLDVLKLDEEYWQTLSQHETALAQVEGLTGL
                     SLR"
     gene            complement(21604..21957)
                     /locus_tag="VGR03_19100"
     CDS             complement(21604..21957)
                     /locus_tag="VGR03_19100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996449.1"
                     /translation="MRIVKSILIAALSLAFAAFAVDCGEAWTSPEEAMQCCDTVLCSS
                     HGASLECCQTMPPIHAPFVLPASAQGVASSTAVVAVLPANCDSHELGSSARVLAAHDL
                     APPGYFSTATAPLRI"
     gene            22584..24680
                     /locus_tag="VGR03_19105"
     CDS             22584..24680
                     /locus_tag="VGR03_19105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEV1996450.1"
                     /translation="MHPQIVRSERGFCPICGMALEPRTASIQEEENPELRDMRRRFWV
                     SAAVTIPVFVAAMGDYIPGQPLKSLASTRTWAWFEMAFATPVVLWGGWPFFVRGWKSI
                     VNRSLNMFTLIGLGVGVAYFYSLVATLFPNIFPASFREEGGVVAPYFEAAAVITALVL
                     LGQVLELKARSETGAAIRALLGLAPKTARLIQEDGGEIDVPLESVKPGDKLRVRPGEK
                     VPVDGVVLEGASSVDESMISGEPIPVEKQPAARVTGATVNGTGSLVMRADRVGADTVL
                     AQIVRMVSEAQRSRAPIQKLADVVAGYFVPVVVGTAVLTFIVWGVWGPSPRMAHALIN
                     AVAVLIIACPCALGLATPMSIMVATGKGATLGVLFKNAEAIELLRKVDTLVIDKTGTL
                     TEGKPTLVAVKPVEGMDEEKLLHLAASLERGSEHPLAAAIVTGAEARKISLIPAQDFK
                     SLTGKGVRGRIEGKDVALGNRKLLEELGVDGGLLRDQAEAMRSDGQTVMFVAVDGRLA
                     GLVGVADPIKQTTPEAIRRLHGDGIRVVMLTGDSRTTANAVAAKLSIDEVIAEVLPED
                     KVNIVKRLQGEGRFVAMAGDGINDAPALAQAQVGIAMGTGTDVAMESAGVTLVKGDLR
                     GIVRARSLSRATMRNIKQNLFFAFVYNALGVPIAAGVLYPFFGLLLSPIIAAAAMSFS
                     SVSVVGNALRLRSVRS"
     gene            complement(25378..25912)
                     /gene="rnpB"
                     /locus_tag="VGR03_19110"
     ncRNA           complement(25378..25912)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGR03_19110"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(26143..28164)
                     /gene="ligA"
                     /locus_tag="VGR03_19115"
     CDS             complement(26143..28164)
                     /gene="ligA"
                     /locus_tag="VGR03_19115"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV1996451.1"
                     /translation="MVKAAAAREEIEKLREEIRRHEYLYFVKDDPQISDAEFDRRVSR
                     LKVLEAEHPSLITPDSPSQRVGGAPREGVQTVRHARAMLSLDNAFSYDALRDWDRRVR
                     EGSGRERIEYIAEHKFDGLSISLQYEDDVLVRGVTRGDGTTGEDVTLNVRTIRSIPLR
                     VETAALKKAGLRADFEVRGEVMMTRKAFDALNRAQEKQGGKIFVNPRNAAAGAVRVLD
                     PAITANRNLDFFAYYLLADGKVPFEKHSESLQVLKQLRFRASDDWKLCPSIEEVLAYC
                     DAWNARREKLAYEIDGVVVKVNSTGIQNELGYTARAPRWAIAYKYPAQQETTVVNEIL
                     VQVGRTGALTPVAVLKPVQVGGVTVSRSTLHNMDEIERLGLHIGDTVLIERAGEVIPH
                     VVRVVKEGKPRRAFRMPRECPECGSRIHKSEDEVAYRCVNAACPAKRKESLLHYAGRH
                     AMNIDGLGEKIVDQLVDKGIVKDVAELYALKLGALAALERMAEKSAQNLLDEIEASKK
                     NSLARLIYALGMRFVGERTGQLLAEHFSSLEELAAATEEQLVEVPEVGPKVAASIAEF
                     FSEPANRNLVKKLRKAGVRPTSEKREVKSQKLAGKSFVFTGGLANRSREEAEALVQQH
                     GGKISGSVSKKTNYVVVGTEPGSKYEKAKSLGVAILSESDFESLLGLHR"
     gene            28254..>29157
                     /locus_tag="VGR03_19120"
     CDS             28254..>29157
                     /locus_tag="VGR03_19120"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEV1996452.1"
                     /translation="MTADLHNALLIHNPRAGSGGRRRLLDEARRILAAGGIETELADT
                     GAPGHATELAQTAGASGRGLVIVCGGDGTLNEVVNGLAASPNGHRVPLALLPAGTANV
                     LAKELGLPWNIPRAAEKLLRGNLREIALGLATPLERPEKKRYFVSVAGAGPDGALVYS
                     LDLGLKARIGVLAYWGEGVRQVVRYKFPKFRIITAEKQLEATLAVVGRTKHYGGPFKI
                     TTRADLLEDRFELAALTTRSALRYLSYLPALWFGDLRAREGVHFWKSEKIVCEPLGTE
                     PVYAQVDGEPLARLPVEFRIVPRAV"
BASE COUNT         5750 a         8529 c         9013 g         5865 t
ORIGIN      
        1 tcaccacggt ggccagcgtc cagagaactt ccatgctcgt gttgccgtgg ctggaggagg
       61 cgcgctggcc gcgatcgcgg aacttgaaaa cagcataggc cagcccgagc tgggaaagca
      121 cgaatacgat ccccgtgatc accagcgtac ggtggaactg cttgtccacc tccacgccaa
      181 aggcggtgat gctcttggga aaccaccagg tcttggccgc gaaaagatag acggtaatgc
      241 caaccagaaa aaccagaagc agggaaaaga tcagcgcggg ggcggtagag cccgcacttc
      301 gccgtcgtct cagcatcgca tgcgctcccg atagacgggt cagcacggcg aacggtgcga
      361 ttcccgtgac agccccagag tccgtgcaca acatcccggg cgcgtactat atacggatgc
      421 aggaaaattt caagcgggtt tcgatagatt tggaaccaag tcgtctcctc gagtttcgcc
      481 atcgctactg cgagagagga ctgattggct catgtggcat agctctcggc gcgaaaaaag
      541 ctgaaacctg aggattccac ccaacctgcg caaggctgtt ctttcatggg acagggacat
      601 gcaaaagggg taacatttga ccggcaaggc cctaagcttg gacaaatttc agccgcatcc
      661 gcgcgattgc tcgtcgaagt ttataaggtg caacaacagc tctaatctgg ttacccgaaa
      721 gaccggtgga gggcacatga agatcagctc ctctcttttc agggtcacgc tggccttggg
      781 gatggtgatc agcctgccgg ccggcatggc ggcgcagaaa ggcgggaaag gcggccctcc
      841 cggaaagccc ggcgccaaca gttcgccgat aaacagcccg caaccgaccc ccacccagcc
      901 gctgctcatt tcgggaaaag ttctggtgga cggcagtggt gccccgccag agccggtgtt
      961 gattgagcgt gtttgcaatg gggttgcgcg aaggcagggc tacactgaca gcagaggttc
     1021 gttccaattc cagctcgatc aaatctcggg tttccaggat gccagcgaga acagcggcat
     1081 cgccggcaac gatactcaga tgacgaccca ggcgcaggac accctgaagg tgaagtacca
     1141 gggctgcgaa attcgggcgg tccttcctgg atttctttcc agcgcggttt cgcttcgggt
     1201 ccagggaagt acctggcaat acgatttggg gaccattttc ctcaagcgca tggagaacgt
     1261 gaccggcacg accatcagca tgaccacgct gaaggctccg ggagatgcca aacgcgctta
     1321 tgagaaggcg cggaaggcct ttgatgaaaa gaagtttctc gatgccgaga aggaactcga
     1381 caaggcagta aagatctatc ccggttttgc cgctgcctgg tcgctgctgg gggacatcca
     1441 tcagcaacag aagcaattgg aactggcggt gaaggattat gggcaggcgc taacagccga
     1501 tccgcagttt gtgaatcccc attttggcct ggcgctgatt gccatgcagg aaaagagatg
     1561 gcaggacgcg gtgcagttta ccaaccaggt cctgaaaatg aaccctgcgg catttccgtc
     1621 ggcttacttc tataacgccg tcgctgcctt caatctggga agagtagatc ccgccgaagt
     1681 gagcgcacgc aaattcaagt cgctggatac ggaccaccgc cacccggaca tctgcctgct
     1741 gctgggccag atcttcattc agaagcagga ctatgcgagt gctgcccagg aaatgcgcga
     1801 ctatctggcg cttgtcccga atgcctcgaa tgctcaggaa gttcgagaag cgttgaagcg
     1861 gctggaagac gcgattgtgg cgacaaagca ttagcaatca gcaaaaagca attggtaatt
     1921 agcccgccgc ggcgggcttt tggcccttcg cattctgctg ctagtctttt tgctgatttc
     1981 gctttacccc gccctagcgc cggcgccgaa ccaagaacct tttgacaaag atcggctggt
     2041 ttccgccgat caggcgactg aagcggtccc tcggctcact gtgttcgctc gacggcatca
     2101 gaactgaggc ggtccctcgg ctcactgcgt tcgctcgacg gcatgagaac tgaagcggtc
     2161 cctcggctcg ttacactcgc tcgggacacg ctttcctaat cggaaagcgt ggtgcggaag
     2221 gggggatgag tttatcccga gaccgccgaa ggcggaccga gggaaaccca cacgcagcgc
     2281 gctctgattc cgagttcgac ccggccttca gcaagattag ttttcttcgc gcgattccag
     2341 gctttgattt gccgttcgcg ggcggcggcg ctgcttcggt cagggtgtgt ttcataccaa
     2401 accagagcca tggggcgtgt gcctttggtg tagccggagc ccttcccgga agagtgatgc
     2461 ttgattctgc tccgaagatt cgtggtgatg ccgcagtagt aggagcgatc ggcgcaaaga
     2521 agcagatagc agcaccaacc caatggtagt tgtgaaacgg cgtctcgagt tctcatgaga
     2581 cttgatgact taggcaaggc ggaaaaacaa agagcccctc ggctcatccg cctgcggcgg
     2641 attcgctcgg ggcaagattt ccttgtcgga aatcttggtg cggaaggggg gattcgaacc
     2701 cccacgcctt gcggcgccac cccctcaaga tggtgcgtct gccagttccg ccacttccgc
     2761 acggaggtga gagttgccat tgtagccgcg agcgaaaaag cccgcaagcg atggagaagg
     2821 atgcaatgcg cctaaggctt gttcgttggc ggtggcggcg gttgctgctg cgtcgcgggc
     2881 ggattctgcg cgccacctaa cggagcagcc ggttgagact gcggctgggc cggaggcgca
     2941 gatttcggcg cgggctgggc cactttttcg aggacggaac tgcccgtcga gccggttctt
     3001 tccttttgca ggacaagggc cagggcgcag accatgaaca tgaccgcgca ccaggtggtg
     3061 gcgcgcgaaa ggacgtttgc agcgccgcgc gggccgaacg cggtctggct gcccgccccg
     3121 ccgaacgccc ccgctagatc cgccgccttg ccgctctgga gtagcacaac gatgatcaac
     3181 accaggcaat tggcgataaa aaaggcgggc aatacccaga aaaaccacgc gctgttggcc
     3241 agccgccagc ccaaagctac gatcccgaaa ccaatcagca acgcgatggc ccatttgaac
     3301 gcagttggca tcttcgactc ccgatcaaaa gttcacaatc gccgcgaacg acaacgggac
     3361 gaggctcgct ccgcccacca gggctccgtc cagctcttcc tgcgccatca acccctggat
     3421 gttgtccggc ttgacgctgc cgccgtagag aatgcgaagt gcggtagcga gcttggcgga
     3481 gaagcgatcc gccgcgcaat ggcgaaggaa gcggtgcgcg gcggcagcaa tctctggcgt
     3541 cgcagtgcgg cccgtgccaa tggcccacac cggctcgtag gctaacagga tacgggagaa
     3601 ttcctcaggg gtcaacgcgc cggggccgcc cgcaaattgc cgcgcgagga tcttttcggt
     3661 agctcccgct tcgcggtcgg cgagcatttc gccgaggcat acgatgggga tgaggccggc
     3721 ggccagggcg gctttcgtct tcttggccac ggcctcgtcg gtctcggcaa agtactggcg
     3781 ccgctcggaa tgaccgatga tgacgtagcg acagccggcc gcagcaagca tgccggcgga
     3841 gacttcaccg gtgaaagcac cctccttctc ccagaagacg tcctgaccgg cgatggcgat
     3901 cggcaaagcg ctggcggcat cgaccgccgc gggaatggcc ggaaacggcg gcgcgacgac
     3961 gatgtcgcag tgcgttgaat gaatgacgag cggcgctaag gcggagaaga aggcgcgcgt
     4021 ctcggcgggc gtcttgtaca tcttccagtt gccggcaatg acacggcgtc tcatagattg
     4081 gtcagggcct cgacgcccgg gagcttgcgg ccggcgagga attcgaggga cgcgccgccg
     4141 ccggtggaga tgtgcgaaat ctgatcggcc acgccggatt gatgcacggc ggccacagaa
     4201 tccccgccac cgacgatgga gatggctccg cttgaggtag cggcagcgat tgccctggcc
     4261 accccgagcg tccctttcgc gaacgccggc atttcgaaaa cgcccatcgg cccgttccag
     4321 acgatcatct tcgttttcgc gatttcggcg gcataggcgg cgacggtttt cggcccgatg
     4381 tcgagcccca tttggtccgc cgggatggct gcaacgtcgc aaaccgtggc cggggagtcc
     4441 gccttgaact ccggcgcgac aacgtggtcc acgggaagca taagcggaaa actgcgccgc
     4501 cgagcttccg ccaggagccg cgcggccagt tcaaccttgt cttcttccac gagcgacttg
     4561 cccaccggca gcccttgggc cttcaggaaa gtataggcca tgctcccgcc gacgagcatg
     4621 gagtcagcga tattcatcag gttttcgacc acttcgattt tctcggaaac tttcgcgccg
     4681 cccagaatgg ccacatacgg ccgcgccggg ttggtgagcg cccggccgag gtattcaagc
     4741 tccttctcca tgagcctccc ggccgcggcc ttctgcacaa agcgggtgat gcccacgacg
     4801 gaagcatgcg cgcggtgcgc ggagccgaat gcatcgcaaa tgaagagacc gtcgcaaagc
     4861 gcggcaaact ctctggagag cacctcgtcg tttttttcct cgccgggttg gaagcggaca
     4921 ttctcaagga gaagaatttc gccgtcggtg agggacttgc tcttcgtctc ggcgatgagg
     4981 ccgacacagt tgggggagaa tcccaccggt ttgccaagga gctcttcgag ctttcttgcg
     5041 accgggcgga gagagaactg cacatccgga gcgcctttgg ggcggccgag gtgcgatgcc
     5101 aggaccagcc ggcctcccat ctgcaccgcg aggcggaggg tggggagcgt ctcgcggatg
     5161 cgtgtgtcgt ccgcgacgaa gccatctttg agaggcacgt tgaagtccac gcggataaaa
     5221 acccgattgc cgcgcaggtt gagatcgccc atggtgagct tgttcatccg gctcggactc
     5281 accgttcccg ccgttagaat tttgaggcca tgaacttgat gagatcgcgg cagcggcagg
     5341 aatagcccca ctcgttatcg taccaggcga ggaccttgac gcatcggtcg ccgacgactt
     5401 tggtgtactc cgcgtccacg attgcggaat gcggattgcc gcgaaaatcg gcggacacga
     5461 gttgctcctc ggtatagtcg agaatgccct tcatcgggcc gtttgccgct gctttcagcg
     5521 ccgcgttcac gctctccacg ctcgccggcc tttccgtgaa caccgtgagg tccacgacgc
     5581 tgacgttcgg cgtgggcacg cgcatcgcgt agccgtcgag cttccctttc agctccggaa
     5641 tcaccaggtg gagcgctttg gccgcgccgg tggagctcgg gatcatggag acggcggcgg
     5701 cgcgggcccg gcgcaggtcc ttgtgcggca gatcgagcag cttctggtcg ttcgtgtagg
     5761 agtgaatggt ggtcatcgtg ccgctgacga tgccaaagga ctcaagcaac actttcgcga
     5821 cgggcgcgag gcaattcgtg gtgcaggagg cgttggagac gacgtggtgt ttcaacggat
     5881 cgtaggtctt gtcgttcacg ccaaggacga gggtatggtc aggatctgtc gccggcgcgg
     5941 agatgatgac cttcttcacc ggcccgcgca tgtgccttct ggcgtcggcg cccttcgtaa
     6001 ataacccggt ggattcaacc acgatgtccg ctccgaccga ggcccaatcg atctcccccg
     6061 gatccctggt cttgaacact ttgaagctct tcccctcgac agctatcgaa tcctcggtat
     6121 gggtcacttt gtgcggcagg ttgccgagga tcgagtcgta tttgagcagg tgggcgagag
     6181 ttttcgcgtc ggtgatgtcg ttgacggcga caaactggat gtcccggtcg tcgagtgccg
     6241 tgcgcatgat gttgcgcccg atgcggccaa agccgttgat gcctaccttg atgggcatga
     6301 atcctccgtc gagggttttc tgcgcgcggc aggcaagtgg cggccccgtc tgcgctgcgg
     6361 aaggttgatg ctagggggcc tcctgagaaa agtcaacgcg gcagggcacg cggggcgatc
     6421 ggttaaacca gagccaagcg cagggaattg ggccttcctg ggcaacccga ccaaacattt
     6481 ttgcctccga cttcgtctaa gaactaggat tgttgcgtct gggcgagagc ccccgcgcct
     6541 gtttttgaat gtgttaggcg ttgggcacgc tctcgagaag gcaggggtag tgcggacctc
     6601 ggccaaaaca ttggggtttg tcgtggtgac ggggtgcctc gcgggcatca tccccggagc
     6661 ttccgcagcc ggccaaaagc aaaccaagcc tgccccgacg cccgtttttg ccaccctgca
     6721 cattcccagt ctggaacagg cacccaagct cgccgacttt ctggatatgc aaccctcccc
     6781 tgccttcgag ggcaagatgc tcaaggtgga caaatttgtt cagcgcgatc ctcacgacgg
     6841 cgccccggtt tcacagcgca cggaagccta tttgggatac accgacaaga atttttacgc
     6901 tgtcttcctg gcatttgatt ccgagccgca gaaaatccgc gcccgcatgc tgcgccggga
     6961 attgattgac gacgacgacc aggtaggcat gttcctggat acgtttcatg accgccgcca
     7021 tgcctacgtt ttttactcca acgcatacgg gatccagcaa gacggccttt ttagcgagaa
     7081 ccagggcccg gattacagtt gggacacggt ctggcgcaca gaaacaaaga tgaacagccg
     7141 cggttacatg gtgctgtttg agatcccctt caagtccttg cgcttccggc agcaggcatc
     7201 catagattgg ggcgtaatgc tggcccgcgt gatcccgcgc aacagcgaac gttcttatta
     7261 cccggtgaat accggcaaac tgcaaggatg gctctcgcag gaaggaacgc tcgagggctt
     7321 tcacggaatc tcgccgggaa gaaacatgca attcatcccc tacgcatccg tgggcgcatt
     7381 ccgcgctttg gatgaacgcg atcccgcagg agttcgcttt accggcaagc atgtagagcc
     7441 caaagccgga ctcgactcaa aaatcgtgat caaggacagc ctggtgctgg acgccacgat
     7501 caacccagac tttgggcaga ttgagtcgga cgatccccag gtcacggtga accagcggtt
     7561 cgaagttttc tttccggaaa agcggccgtt cttccaggag aactcgaatt actttcagac
     7621 cccgctgaac ctggtcttca cccgccgcat tattgatccg ctctacggcg tgcggctgac
     7681 aggcaagatc gaccgctggg cgattggaac tttcgtggcc aatgaccgat cgccgggcaa
     7741 gagcgtgatt gacaccgacc cgctccgtgg gaacaacgct ttatttggcg tgctccgtgt
     7801 gaaccgggag gtgggaaagg acgacagcat cggattcatt ttcaccgacc gggaactgca
     7861 caccgtgccc aacagcttct gcacgaccac aatttgcgcg gtgggattca accgggtagg
     7921 cggatttgat acccagatca aaatcaaccg aaactggcag atgaatgcgc aggccgtgac
     7981 cagtgaaacc aaattcagca acggcatcca gaaagccggg ccggcttatc aggtctacct
     8041 ggagaggagt tcccgagacc tggaattcaa ttccttgtat caggacattt ctcccgggtt
     8101 ccacaccgat acgggtttcg tgaaccggac tgattttcgc cgcttcagca acttcattgg
     8161 atacaaatct caccgtaaag gcaaactcct ggtcgcccac ggtccaagcg tatttgaaca
     8221 ggccctctgg gaccacaacg gaactcgcct gcaatactta ttcaatggca attatgagtg
     8281 ggattttcag cggcagagct atttcggatt ctttggcaat atcgaacatg agcggctgcg
     8341 tcccgtggat ttttcctcgc tcacggacaa tcgcgattat ggccaccatc tggtcgggcc
     8401 ggttttcggc acgcagtatt tcaagtggct caacctcaac ggcgaaatag acctgggtac
     8461 ggcaactaac tttgtgccac gtgccgggcc gccggtgctg gccaatttca ccgccattat
     8521 tctgcgtgga gtcgtgcgcc caatcaaagg gctcaccatt gaaaatacct atctcctgac
     8581 gcggctgctg gaccagcaga cagacctcaa catctttaac aatcacatca tccgctccaa
     8641 gtggaattat caattcacga aggaattctc actcaggatt atcggccagt acgtcaccac
     8701 gatttccaac ccggcgttca ccaccttgcc aagcgccaag aagttcaacg gcgacgtgct
     8761 gttttcctat ctgctgcatc cggggacggc agtctatatc ggctacaaca gcgacctgca
     8821 gaacctggac ccgacgctgg gactggaccc caacggaaac ctcctccgca cgcgcagccg
     8881 gttcctcaat gacgggcggg aaatcttcat caagatttcc tatctgctcc ggtactgagg
     8941 cgaggcgcgc ccccacagtg tttcttaaag atgccgccac gatggggacg ctgacaatcg
     9001 agcggagggc tattgaggga actgcttgat gctggtgacg cggtcgccct ggaagcggac
     9061 gaaaacagtt ttcgacggcg gctggccgta aatccaatcc tcgatctcgt tgccgtcgga
     9121 atcgcgctcg cgaatcttgt gcgccggcct gccgatggcc gccacgacca tctcgcggtc
     9181 catcccgagc acggggtgcc gatcctggat tgcctttttc atttccgggg ccagggtgtc
     9241 gtaccactgg acagaggcgg agcgctgctt ggagaaatcg aggagcggcg cgagaaggtt
     9301 cttcaggtcc tccggagaga ggtccgggat gggctgattg aattcgagga aaatcgtgct
     9361 gcccatcccc gacttgaggc caggagggcc ggtctgcggc tgggtggttt tcacggtggg
     9421 taggccgccg aaatcgatgt gaatgcgatc gcggaagcgc tttttgacac ggccgccgcc
     9481 gttcacgtcc acaacgatat cgtgctcgcg gaagtacagc atggtgatct gcacgttgtc
     9541 gcccgtattg acggcggcgc cgtgcgtgtt gacggcgcgg tccagcaact tctgatcgag
     9601 cggcttgccc acgtagagga gaaagccttc cttcccgccc gggagcggtt tgacggcctt
     9661 ggcaaactcg cccgagacgt agcggaccag cgcaagctcg ctttgcggac tcaggcggct
     9721 gcccggagac cgagctgagg attgctgctg cgattgcccg ttaccgagga cggggagcac
     9781 ggaggggagg actgggatcc ccagccccgg ggggaaatcg ggcgctggtg ccgggctcgg
     9841 ccgcaggcga taaatcatgg gcatgtccgc atccgcgaac aacaagaaga tcagcggaat
     9901 cgtcagccag cctcgcatac aaccccgatt gtatgatgcg cgcgcccttc cgtacagatg
     9961 ccagtgtgag caccctataa tgtgctagcc ggcgtgcacc tgcactccga ggcggtcaat
    10021 cttgatgggc aggagttggc ccccggcgcg ctccacggcc gtttcgacgg ccggacgcga
    10081 gtccggctcg atcagcagga ccacgcagcc tccgccacct gcgccgcaaa ccttgccggc
    10141 gagcgcacct tgcgaacgcg cgccgccgat gattgcgtcg atcgtgcgcg tcgagatggt
    10201 cggcagattc ttcctgcgga aagaccattc ttcgcgcatt attttgcccg cggcattcca
    10261 atcgctgcgc tccagagcgt ggcgcatctc ctgcgcgatc gcggaaatct tcgccaggtt
    10321 gcgctggatt cccttattgc cgttaatgtg cgctttgaaa acttcccagt tgttgacgcc
    10381 ggactgtctt ggcttccccg tgtagcagag gacgagccgc cgctcgagct cgtcgagatt
    10441 ggcccgcaac tcgctgcgcc gctcgctgcc ggggagcagc tcgataactg cagcgccgcc
    10501 gaaggcgggc gggtagtggt cctgtgtgcc ggtcggcacg cggataacga ttgcttcggc
    10561 gtcgcggctg atgtggatcc actcgacttt gctcttgccc gcgccagtgc acttgtcgag
    10621 ggccgcgcag atagcaaccg ccagggcgct ggagccgcca atccccgctc cggccggggc
    10681 ttcggagtcg gtggtaagcg tgaagccgcc agccggctga aagatcttga tgagctcggc
    10741 gagtagcggg aggcggggct tctttgcatc ggctaaggcg gagagcgaag cgaagctctc
    10801 ttccctcttg gtgtcgcggg agacgagctg gatcttcgcg ttgcccgccg ggtgcgtctc
    10861 caggacgcag gaggcgtagc gcgtgatcgc cgcgttcacc gtgactgcgc ctgggtggaa
    10921 gagatagagc ggccaaatgt cgagcgtgcc gccggccaga tccacgcgcg tcggggctct
    10981 ggcctcgatt ctcacttgtt gccgcttccg acggcgcgga tgcgcgacgg ggtaagcggg
    11041 ctgggctcct tctcggcctg cgcgatgttc gcttcgaagc agcggcggca acgggcttcg
    11101 tacatcccgc tcgccccgac gacgatcagc tcttccgatt ccaccaagcg ctgcgtgtgc
    11161 acggcgggat tgccgcagcg gacacagatg gcgagcagct tggtgatctc ttccgccacg
    11221 gagagcagcc ggggcatcgg ctcgaagggc ctgccgcgat aatccgtatc caggcccgcg
    11281 acgatgacgc gcttccccat gtccgcgagc ttggtgcaaa caccgacgac ttcttcgccg
    11341 aggaattggg cttcgtcaat gccgacgact tcggttcgcg ccgtcacgcg cgccaggact
    11401 tcctctcccc tcgtcacgtt ctccgaggcg attcccagcc cggagtgcga tacgatctcg
    11461 ttcgccgcat accgcgcgtc aatcgccggc ttgaagatct gcacccgctg gcgggcgatc
    11521 tccgcgcggc gcaagcgccg gatcagctct tcgctcttcc cggaaaacat cgggcccacg
    11581 atgacttcga tccagcccat attgcctttg acgatgtcca cgttcgcagc ctccgttttg
    11641 gtatcgaacg aatcccggca tccatcccta gcgcgccgca ccggctgtgc gatacatctt
    11701 cgcattgtag aacggagcga aggcgctcac caccgccacg cgaaaagcct cctgccgggt
    11761 gatgaggtcg cgggagagca cgccccaggc gccctgtgca tcgcggattc ccgccgcccc
    11821 ggcaaagcgg tcgatggctg cggccagttc cactccgtct tcgagaactt cacgggcgag
    11881 cgcctccggc atttcgattc cgccgctgca tccaaagaat cccagagcgc catcgctcac
    11941 gtaagcccag ctttccagga agactcggcg ccggccatct tcgtgcaaga cgtcgagccc
    12001 gccctcgagc ccgacgaaat agcgccaggg ctcggcgtga ctccgcgcga gttgaatcag
    12061 tcgttcggcg cgccgccgcg ctccgcgcat caactcttcc cggctgagcg gagtatggct
    12121 gactccgctc tccacttcca cgcccgcaac gtcaaacgca gcgccgggat cgagcgccgc
    12181 cccgatgaac cccagcgcct ctcggacggc attcagcttc gggcggcgcc gactgccgac
    12241 cgcgacgatg atcttttcca tccgcgtccc tgacctgccg agtgagattg taacggcagg
    12301 tcatggagaa tttcacgcgg aaagaatgag ggggacgaga gggttgagga aatccgagga
    12361 ggcatctgga gcggcctatc ggaatcgaac cgacacctga accttggcaa ggttccgtac
    12421 taccactata ctaaggccgc ccaacgcact tcttttatag cactttcact cacagcccac
    12481 aagccgacct gcgagcacac gtgcgcgccg ttccgtctat ggcagcaaac agcgaatcag
    12541 cgtctctgat cttctaggag agctggctga cccgtgcgct cttgaccacg ggcgactttc
    12601 tctgccgttc ggcgaagcga gcagctttgt cgcggtccgt gtacacgcgg ccgaattttt
    12661 caccgtccgc atagtacacg gtaacgagcc agtgatgatg cctgcggcga cgatcactgc
    12721 tgaaggaagc tttcttcctt ttctttttca agattcgtcc cctcgatgaa gataaccggg
    12781 caactatcgc agttctgccg atcatacgca agtaacgtgt gagctactga catggtggga
    12841 atatagggcc gagctatctc catcccatac aactgcgccc gcaagaattc cggattaccg
    12901 atgaaagaga ccgctaccga tacgccatac ccgttgcagg ccccgccaac aactgttgtc
    12961 ggcgggcgtg ctctcgcttc taaggagatg aaacgtcagg tgcaggccat aggtatcatc
    13021 atccgccgga gtgaaggccc cgatgtgccg ttcgattttc ggcgtggccg cgccatttcg
    13081 agaatcagga gccggcctca ccaaaccatc cgggattctg ccgcatccaa cgactgtgcg
    13141 agatagcggg gttttgcctc ccgggccgca aggtgccaac ggggggagaa aataggcgaa
    13201 aagcgaaaca tttttgagtt tattgcgtct tatagaggtg agggggcaag cggaaaaggc
    13261 ggcaattatg aacattctga aacatccgcc catcgttgag gacctgagga accactctcc
    13321 ggagcagctt gccgagctgc ggatgctcct cgaggcggag gcgcccgcgc gccccgatcc
    13381 gcggcgcccg cgtttctttg agatccaagg gtcaaccagc gtattttaca ttttcaaata
    13441 tccgagcggc acaaaggtcc tcctgattgg cgtgtgggat cgcgaccggg tggccgaact
    13501 cgtgacctgc gcctgcccgg ctgcttgaac caagccagca caatcggcag ttctcaatca
    13561 ctccctatct gagaacggaa gggcccgccc tgctgtaagc gggcgctcgc ctgctcattt
    13621 ctcttcgtcc agatcgagcg cggcggagtt gatgcagtag cgcaggccgg tcggccgcgg
    13681 gccgtcttca aagacgtgac cgaggtgcgc atcgcaacgg ctgcacttta cttccgtgcg
    13741 cttcatgccg tgagaagcgt cggtttccgt ctcgatggcg ttctggtccg ccggcgccca
    13801 gaagctcggc caccctgaac ctgagtggta cttctcgccg gaacgaaata gcggctgtcc
    13861 gcaacagacg cacttgtagg tgccgggagt ttccgtatct tcgtgttctc ccgagaaggg
    13921 cggctccgtg cctctttgcc gggtcacgta atattgattt ggcgaaagtt gcttcttcca
    13981 atcggcctcg ctcttgctca acttgtcctt cacgctctct cctctccaac cgcgggacgc
    14041 cacccttgtt ggatgactgg atgatcagat tcggcgcact ccttctgcgt tataccggcc
    14101 gaaagcgata ttgaatcata atcatggccc gggcaagctg gccatgtctc tcccgcgcga
    14161 gggattcccc actcgtctcg ccagcctcat ctggacagtt ccacggcttt cgtttagctt
    14221 cgctctcggc gcgaagcgta gcgttgcgcg cccgtgtgct gcttctgttg gaggcgcaat
    14281 gtctctccga ggcctgccgg ttcggcgggc ggtcctgcgc gggaagggtg aagtgccgga
    14341 gctcgagggc cgcgcgggca agctacggct cgacttgaac gaaaacacgg ttggctgctc
    14401 ccgggccgtg acgcgcgcgc tgcggcggct ctctccgcag cagatcgcca tgtatccgga
    14461 gtaccggcgg gtgacgtcga ggctggcgcg cggggagggt gtgcggccgg aagagatgca
    14521 cctcaccaac ggcggcgacg acgcgctgcg catcgtcttt gacgttttcg tggacgcggg
    14581 gagcacggtg ctattccccg agcctacttt tcccatgtac cgctttttcg cgggcgtgta
    14641 tggcgcgcgc gtgattgcac cgcgcttcga ccgcgagatg aatttcccgg tggcggagct
    14701 gctcgcagcg ctgctccggc ggccgcgcgt tctgttcctc gcgaatccga ataatccgac
    14761 tgggaacatg ctcccgccgg ccgatttgcg ccgcatcctg cgcgctgcgc ggcgcacggt
    14821 ggtcgtggtg gatgaagcct acgtcgagtt ttccggatgg agcgccgcag cgtggattcg
    14881 ccgatacccg aaccttgtcg tcgcgcggac gtattcgaag gcggcggggc tggccgggct
    14941 gcggctcggc tgcctcatcg cgcgcgccga ggtgacggag cttttccgga gggttgcccc
    15001 gccgttcaac gtgaacgcgg cggcgctgat cgcggcggac gctgccatgc gggatcgcgg
    15061 ggcggcgcgg cgctacgtcg ccgaagtccg gcgggcgcgc atcgaattcg agaagcagct
    15121 cgcgcggctc ggttatcgtt cgttgccgag tgtggcgaat tttgtcctgg tggatttcgg
    15181 cccgcgcggg ccggaaatcg ttcgccgtct caagcggcaa ggcattttgc tcagcgaccg
    15241 cagccgcgat tctcgccgcc ggggaatggt gcgcgtctcc atcggcacgc gccgcgagat
    15301 gcgccgcctc gccgccgccc tcgcccgcct cgcacgcaag taattacaaa cagaaattcc
    15361 tatgcatttg acgagggttc tctgggctgc aaggttccac gacgcagcgc acgctccttc
    15421 atcaaagcga aaaacacggg caccagaatc aggacgtgaa ttgttgatgt aatcatgccg
    15481 ccgacgatgg gcgcagcgat gggcttcatg acgtcggaac cgattccact ctcccaaagg
    15541 atggggatca agcttgcgag gacagccgtc acggtcatca gcttcggacg caaacgctgc
    15601 acggcaccct cgattacagc cgcttcgata tcctcgtgtt tgagtggaat gccggaagag
    15661 aggcgcctat cgagcgattc gtggagataa acgaccatga ccacgcccgt ttcaactgca
    15721 attccgaaca gcgcgatgta gcccacccag acggccacgc taaaattgta gccaagccaa
    15781 tactgaagaa tcagtccgcc ggtcatcgca tagaacgtcg ggaaaataag gacagcggcc
    15841 tcggtggccg agtggaagac catatagagc agcaagaaga tcacgaaaaa cacgagcggc
    15901 acgatgaact tcagcctttc tttggcgcgg acttcgaatt cgtactctcc ggaccacttg
    15961 taggtgtatc cgggcagtag gcgcaagttt tcgttgagga gccggttggc cttggcaacg
    16021 aagccaccgt agtccttcgt tgcgaggtct agataaacat agccggtgag caggccgtct
    16081 tcgtcgcgaa tcatagccgg gccgcgggag aagtagacct tcgcaacttc agagatggga
    16141 atctgcgcac cggcgggcgt ggcaatgatg acgcgccgca gcttttcaat gtcatcgcga
    16201 aagtcccggc tgtagcgcac gttgatggga taacgctcgc ggccttcgat gttttcagcg
    16261 acgttttcgc cgccgatgcc ggacgtcacg gcacgctgga catcggccac ggtaaggcca
    16321 taacgcgctg cttcggggcg gttgacttcc acgttgatgt aaaagccttc tgcgacgcgt
    16381 tccgcaaaga tggaacggag ttcagtcatt ccgcccagga cctgctgtaa ctgagctccg
    16441 gctcgctgga taccttcgac attcggccct tgaatcttga ttcccacggg tgtcttgatg
    16501 cccgtcagct ccatatcaag ccggttttga accggcatcg tccaggtatt cgtcagacct
    16561 gggaatttga gcttttcgtc catctcgcga atcagctttt cataggtcat gccagcacgc
    16621 cactgctcgc gcggcttgag cataatcgtt gtgtcgtaca tatcgagcgg agcgttgtcg
    16681 gtgggactgt cggagcggcc aacggcgccg aagacacttt cgacttcagg gaaagacttg
    16741 atgatgcggt cctgttcctg gagcaacgcc gaggcttggg tgatcgagat gccgggaaga
    16801 gcggtcggca tgtagagcga ggagccttcg tagagcggcg gcatgaactg actgcccatg
    16861 cgccacgcca gcggcagtgt cacaaccagg aaaatcagat tcagaaagag cgttgttttc
    16921 cggtatcgga gacagaaacg gaggacaggc aggtacaagg cctgtgtcca gcgtgaaaac
    16981 ggatttgcgg attccggccg gagattgccg cgaatgaaaa tcaccatcag gaccggcacg
    17041 agcgtgatgg caagaatcga agagaacgcc acggagagag ttttcgtcca tgcaagggga
    17101 cgaaacatgc gcccctcctg cgcctcgaga aggaataccg gcaggaacga cacgaggata
    17161 acgagcaagg aaaagaagat ggccgggccg acttgtttcg ccgcacccat cagtatgcgt
    17221 cgcctttcct tttctgtcag cttggctccc gatgctcctg ttgtcgcaat gtgttccgac
    17281 aagtggcggt agccgttctc gaccatcaca atggctgcgt cgatgagcac acctatggcc
    17341 agcgccagtc cgccaagcga catgatgttg gaactgacgt gcaggaagta catgggtatg
    17401 aaggacgcca ggaccgcgat gggcagagtc agaatcggga ttagcgcaga gcgaaaatgg
    17461 aacaggaaaa caatcgtcac aaagctgacg atgatggctt cttcgagaag atcgcgttgc
    17521 agcgtttcga tggatgcctg aatcaatccg gaccggtcat agccggagac aatctcgaca
    17581 ccaggtggga gcgacggttt gatttcggca agctttttct taacaccatc gattacgtta
    17641 agggcattca ttccgtagcg catcacgatg attccgccca cggtttcccc ttcgccgttc
    17701 cactcgacga caccctgccg aatatcaggt ccgaacgtga ctgttcccag gtcgcggacg
    17761 agaaccggcg tgccgttctt tgtagcaaca ggcacggttt cgaggtcttg tagcgagcgg
    17821 agatagccga gcccgcgaat catgtattgt gcgccgccca tttcgagcac ccggccgccg
    17881 acttcgttcg tgctctcgcg gacgcggtcg atcaccatgg aaagcggaat gttgtaggcg
    17941 cgaagcttgt tcgggtccag gttcacctgg tattggcgaa cgaatcctcc aatggaagcg
    18001 acttccgcga cacccgggac cgtttcgagc tgatatcgga ggtaccaatt ctggaggctg
    18061 cgcaagtctg caaggctgtg ctggtgcgag cggtcaatga ccgcgtactc gtatacccac
    18121 cctgcccccg ttgcatcggg cccgatcatc ggagacacgt ttttcggcaa gaccccctgg
    18181 agttgctgga tgtattcgag aacccgcgaa cgagcccagt agaggtcggt gccatcctcg
    18241 aaaaccacga agacataggc gtcaccgaac atggtctgcg cgcggacggc ctttacatgg
    18301 ggggcggcaa gcaacgtggt gacgatcgga taggtgactt ggtcctcgat gatactcggc
    18361 ggctcacctt cccaggggat gtgcacgatc acctgcacat cggaaatatc cggaagggcg
    18421 tcgagcggaa tattcttgag cgaccagatc ccggcaagaa cgagcagcaa tgttccggta
    18481 aaaacgagga atcgattgct tgcgcaccaa tcaatgatgc gggagatcat ggctacattc
    18541 ccccttccgc gttgatgctc agttgcttgg tcgcgaggag ttggccgttc ttttttgcga
    18601 ggattgtgac ttgccaggtt ccgccgctct caagctgtcc agaaccttca tagaggcctt
    18661 tgcccttgtc gctgagggtg acaggtgtgc gcatcgctgc catgcccatt gcgggcatcg
    18721 ccggcatgaa aaatgtcacg ctgacttctg ccccggaaat gggcgctcca tttgcgtctg
    18781 taagtttcac gcggaagacg tttccgccct tatggggcgg ggccggatcg gaactcaatt
    18841 cgacgtttac ttgaggagta ttcattgctg acgccgcgcc cgcgccggga ggtggaggga
    18901 caaacgaacc gatggcggct tgcagctggc tttcggaatc aatcaggaag ttagccgaag
    18961 tgacgatgcg ctcgccggcc ttcaagcctt tcagaacgac atattgatcg cctgcctgcg
    19021 gtcccagttg cacgtcacga ggctccagat aacccgcacc tcggtccacg aaaacaatct
    19081 gccgcgcccc tgactgtaga actccgctca cggggatgac gagctggttc ccgagcggca
    19141 cctgcaagac cacgttcacg tacattccag gcttgagcgt gagattggga tttgaaaaga
    19201 cgaggcgtac tcgtgccgtg cgcgttgtgg cgtccacctc ggggtagatg aaatcaactc
    19261 ttcctcggaa cacgcggccg gggtaggaat ccacagtgag cgcggttgga gctcccactc
    19321 ttatgcgccc caggtcgttc tgaaagacgt gagcaaagac ccagacggtc gagagatcgg
    19381 cgatcgtgta aaggcgcgtg tcgggctgca ccgtcaggtt tggcagcgca ttgcgctcag
    19441 tgatgtaacc cgtgacaggc gaatcgattt caagcccctg ctcgacttgg cccgaggatt
    19501 ccagccttgc aatctcacgc tgcggcacat tccattgcct cagccgatcc gcggatgcgt
    19561 caaccaacga ggcaatcccg gcagcaacgc ccggcacggt gctctgcgag agggcttgcg
    19621 cgttttgcct tgcaaccagg tattcacgtt cggcggcggc gagttcgggg ctgtagatgg
    19681 tgaagagagg ctgccccttg cggacgtact gatacgtggc atcggcaaat actttctgta
    19741 tgtagccaga gaaacgcgtt tggacatagg atagccgtgt ttcgtcgatg gcgacggtcc
    19801 cggtcgtccg gatctcatcc tgcaccggct tccgtgcgac ctcgccgaac ttcacgccga
    19861 tggattgcaa ccgctcggcg gaaagctgga cgggcgcgag aggagtctct gcaggtggcg
    19921 gcgccgaacc gagagaacca ctttgaaccg gttgcttcgc agcgttcggc gcggaaacag
    19981 ccgcgtgttg cgaaccggca gaccttcgga cgttccacca gattccggcc agcacgacga
    20041 tgaagaccaa attcccaatc agtgcaagga agaacgcttt gcgatagttc ctcatgatct
    20101 tgctccctgc tgcgtcattc agcgcagcga caatcccgta agtccttcga cctgggccaa
    20161 tgccgtctcg tgttgcgaaa gcgtctgcca gtactcctcg tcgagtttca gcacgtcgag
    20221 gaaggaagtc agcagcgcct gaaaatcctg gcgattcgat tgatatccgg ccattccagc
    20281 ctgcagctcc gcacgcgcct gaggaattag cccctctcgg taaatcttca gaagctctgc
    20341 tgacttctcc gagctcaaaa actgctggcg gagctgaaaa gcgacttgtt gggtctgcgc
    20401 ttcgtgctcg cttttcgctc gactctgatc cgcttcggcc tgagccagtt cgggctgctg
    20461 gcgtcgccct cggtagatcg ggattcgcac accgagtgtc aacatgtagt aagctcgaaa
    20521 ttgagtggga tcggtgcgct gccacatgta ttgaacgttg aagtccgggt agaagtcctt
    20581 cttggctagg ttcacctgca atccctgcct ctccaccatt ttttgggcgc cggtgatttc
    20641 ggggttgttg gccttcgccg ctgacagcca ctggtcatac gtataggcga ccttggactc
    20701 ggtgagttcg acggtctcga tatccggcga cgactgcgga cggttgagaa gctgcttcat
    20761 ctccgcctga aacctccctg cttcgagctg gttcgtcgtg atttctctga gcagcttggt
    20821 gcgttcaagc tgcgcctgca gaacgtcatg ttgatttccc ataccgctgc ggtaacgggc
    20881 ttcggcggac tgttcgatct gttggagtaa ctgcccatct ccttccagga tcatctgcct
    20941 ctttgcgaga tagcccaatt ggaagtaggc catcttcacc gctgccagaa ttgcccggcg
    21001 gacggattca tatttctgct gagtcacatc agcgtctttc ttggcaatct cgccgcgaag
    21061 gcgcagtttt cctggatagg gaaagtcctg agagaccccg aggccaatat aggcaaaatc
    21121 gctgttgctg taacccgcga aggggcgtgg actgcctacg ctcacatgct gcagcacgaa
    21181 ttgtggatcg ggcagcgtcg acacctgcgt cggaacttgc tgagccgatt cccagccatg
    21241 ccttgcggcc tgaatctgag gattgttgcg ctcggcctcg gtcaacaagt cctgtagcgg
    21301 agtgaccgtt ccaggatgcg gaatcgcttg cggtggcgat gcaacttgag cggacccgcc
    21361 ggtttgctct tggcctgcgg ctctgggtgt gtgcgcgaaa aaaacgagaa gtacggtaat
    21421 gcagagaatg cgcacgacaa ggaattcatt gtgtgatttt tcaccgtgta atgccgacat
    21481 tttattcccc tcctcaaaaa ggtctgagag tttcagcgat cggaagtacg gcctcgtccc
    21541 gtaaagatga cggtgcccgg ccacacgaat ccgtttaggc gcaggccggg tgaagaggtc
    21601 agactagatg cggagaggag cggtggcggt tgagaaatag ccgggtggag cgaggtcatg
    21661 cgccgcaagg acgcgggcag aagaacctaa ttcgtgcgaa tcgcagttcg ctgggagcac
    21721 ggcgaccaca gcggtcgaag aggcaacgcc ctgcgccgag gcgggcagca caaacggcgc
    21781 gtgaatcggc ggcatggttt ggcagcattc caggctggcc ccgtgtgacg agcacagcac
    21841 ggtatcgcag cactgcatcg cctcttcagg cgatgtccac gcctccccgc agtcaaccgc
    21901 aaaggccgcg aaagcgaggc tcaatgccgc gattagaatt gacttgacga tgcgcatcca
    21961 ctttcaattt aggcctcttc tcggccgaag tcaagaaaga tggtccccgc ccgcaacaaa
    22021 acttcatatg cttttctctt atatctcttt cacacactgt ttggtacaag aatcggtgcg
    22081 ggcaaacgac gcctgtcgcc gaaagagatc ttgcaagtgc gtctcgcttg tggagaaatt
    22141 gatagagccg acggatggcc aggcacgatt gattcgatga ggaagatctg gaggttctca
    22201 tggacgcagt aaatgatggg aagggcacgt ctaagaagcc ctctcagctt cctattggcg
    22261 ctgacgcagg gaaagaccca gtgtgtggga tgactgtgaa cccaaaatcg gcttctgctc
    22321 actccgagtt cgaaagccag acctactact tttgcagtgc ccaatgcagc gagaagtttc
    22381 gaatcgagcc cggcaaattc gtaaagcgag tggttcgctc tagcgaaaag cagtccccat
    22441 cggcgggcga ggatggggaa tatacctgtc ccatgcatcc cgaggtacgc cagaagaagc
    22501 caggttcatg tccgaagtgc gggatggcct tggagccagt caaaccctcg gcacctggat
    22561 cgaagacaga atacgtttgc ccgatgcacc ctcagattgt gcggtctgaa cggggtttct
    22621 gccccatttg tggcatggcc cttgaaccca gaactgcttc gattcaggag gaagaaaacc
    22681 cggaactccg ggatatgaga cgcagatttt gggtcagcgc cgcggtgacg attccagtct
    22741 ttgtcgctgc aatgggcgac tacatcccgg ggcagcctct gaagagtctt gcttcgaccc
    22801 gtacgtgggc gtggtttgaa atggctttcg ccacgcctgt cgtgttgtgg ggtggttggc
    22861 cctttttcgt tcgcggttgg aagtcgattg tcaatcgcag tctcaacatg ttcacgctga
    22921 tcggattggg cgtgggagtc gcctatttct acagcctcgt cgcaacactt ttcccgaaca
    22981 tctttcctgc ctccttccga gaagaaggcg gtgtagtcgc gccgtatttt gaggccgccg
    23041 ccgtcatcac cgcactcgtt ctgttgggtc aggtgctgga actcaaagcc cgcagtgaaa
    23101 cgggcgccgc catccgagcg cttctcggtc ttgctccgaa gactgcccgt ctgatccagg
    23161 aagacggcgg ggagattgac gtacctctcg aaagcgtgaa accgggcgac aagctgcggg
    23221 ttcgtccagg tgaaaaagtg ccggttgacg gagtcgttct ggaaggagca agcagcgtcg
    23281 acgagtccat gatatccggc gagccgatcc cggtagaaaa gcagcccgct gctcgtgtta
    23341 cgggcgcaac ggtaaacggt accgggtcgc ttgtcatgcg tgcggaccgc gtcggcgcgg
    23401 ataccgtgct cgcccaaatt gtacggatgg tgagtgaagc tcagcgcagc cgagctccga
    23461 ttcagaaact tgctgacgtg gttgccggct atttcgtgcc cgtcgtagtg ggcactgctg
    23521 tcttgacctt catcgtctgg ggcgtttggg gaccgagtcc gcgcatggcg cacgccctca
    23581 tcaatgctgt tgcggttctt atcatcgctt gcccttgcgc gctcgggcta gccaccccaa
    23641 tgtccatcat ggtggcgacc ggcaagggcg cgaccttggg tgtacttttc aagaatgccg
    23701 aggcaatcga gctgttgcgc aaagtggata ccttggtgat cgacaagaca gggactctga
    23761 cagaaggaaa accaacactg gttgcggtga agcctgttga gggtatggac gaggaaaaac
    23821 tgctgcacct ggccgcaagt ctggaacgcg ggagcgagca tccgttggct gccgcgatcg
    23881 tcacgggtgc cgaggcgcga aagatctctt tgataccagc gcaggacttc aagtcgctta
    23941 ccggcaaggg tgtcagaggt cgcattgagg gcaaggatgt tgccttgggc aaccgcaagc
    24001 ttcttgagga attgggagta gatggcggtc ttctgcgcga tcaggcagag gccatgcgaa
    24061 gtgacggcca aaccgttatg ttcgtggccg tcgacggcag attggcgggg ctggtaggcg
    24121 ttgcggaccc catcaagcaa acaacaccgg aagcgattcg ccggctgcac ggggatggta
    24181 ttcgcgtggt catgctgacg ggtgatagcc gcaccactgc gaacgcggtc gccgcaaagc
    24241 tttccatcga tgaggtgatt gcggaggtct tgcccgaaga caaggtcaat atcgtgaagc
    24301 gacttcaagg tgaaggccgc ttcgttgcca tggccggcga cgggatcaac gacgcgccag
    24361 ctctggcgca ggcgcaggtt ggaatcgcca tggggacggg cacggacgtc gcgatggaga
    24421 gcgcaggagt cactctggtg aagggcgatc tgcgcggcat cgttcgcgcg cgttccctca
    24481 gccgtgccac catgcgtaat atcaagcaga atctcttctt cgcttttgtc tacaatgcgc
    24541 ttggtgtgcc aattgcggca ggtgtgctgt atccattttt cggattgctc ttaagcccaa
    24601 tcattgccgc ggcggccatg agttttagct ctgtatcggt tgtagggaat gcactgcggc
    24661 ttcgctctgt tcgctcgtag ccagactctt gcctgcggcc ctgcggcgtg acgatgtgcg
    24721 gcagtacggg gtcgatagaa gtggcggatg ggcctgtaag ccggattctg tggaacaggc
    24781 cccggagaca ggaagcccgg gggggccgac tgccgattcg ctgaaagccc tcgtcagcct
    24841 cccatcggag cctgaaacca cagggagagc cgacgcaaag cccctggccc tcgacggtgg
    24901 ctgctttctt gcgcagccgg cgcaaaagga taaggctctt caaacgcagg ggagaccctt
    24961 tgtggggcct cggcgtgcgc ttgggaagcc attcgaaggc tcagaaatct ccgcgcgaac
    25021 cacacgagag tcaccacgat agtggcatga agaaggaggg ttcccgcctc gtaccaattc
    25081 attgctatcc aatcatagag ttcattcatg gcttatcaca cctcgctaaa cgccccatgt
    25141 actctgagac ggcagactgc ctcagatatg cctaggttac agactctatc cacggcggga
    25201 tactggtggg aagaccaaga aaggggcagg ctgttgtgaa ctggccacag cgtccatccg
    25261 cccaggcgga gagggcaaga gacttgagcg agccgacgca ccgaattcgg tgccaatgcc
    25321 gagccctcgc ttgagcatgg gggcggtgcc tgtaagttat tgaaagctaa agagaagatg
    25381 gcggatgggc ctgtaagccg gattctgtgc ccgcggcgtc cgaaaaacgg cctgccgcag
    25441 cggcgatcat tcctctagtc ccgcaatcgc tcgcgggatc cagcagccta cccgagggtt
    25501 cacattgccc cttgcgcgct aggcgcaaag ggcaagcccc ttcgagccga caaaatcggc
    25561 tctccgggta aagggccggg ccagccctcc cctcctattt ggccttgcac cacgcggggt
    25621 ttgccatgcg tccggtctct cgaccggacc ggtgggctct taccccacct tttcaccctt
    25681 accatgccga gcgtgcgttg cgaagacgtc ccgcaggttt tcctgcgcga tgtcaccggg
    25741 ctgcgctctg ccggcggtat attctctgtg gcgctgtccg tagcccgtgt agcgccggcc
    25801 cttagaccgg catcccacag gccccctggc gttaccaggc gcgttgccca acggtgtccg
    25861 gactttcctc ccgccccaaa aactcggggc cagcgatcgc ccggcccatc caccactccg
    25921 aattatacgc cacttggcaa cggcggagcg ccccgatttc gtgacctttg tcgagccgcg
    25981 ccccgcctcc gcaccagacg aatcacggaa cgctttccag tgctgcctct gaacaaggaa
    26041 ttgatgtgtt gccgcggcgc gcggaactac ttcccggcga ggacggggag catcacggcg
    26101 tcggaaaacg cgccgccgat tctcactctt ctcccgtccg ggctatcggt ggaggccgag
    26161 caaagattca aaatctgatt cggagaggat cgccacgcca agggacttgg ctttctcata
    26221 tttcgagccg ggttcggtgc cgacgacgac gtaattggtt ttcttgctga cggagccgga
    26281 gatttttccg ccgtgctgct ggacaagcgc ctcggcttct tcacgggagc ggttggcgag
    26341 gccgccggtg aagacaaacg atttcccggc gagcttctgg cttttcactt cgcgcttttc
    26401 agacgtgggg cggacgccgg ccttacggag cttcttgacg aggttgcgat tcgcgggttc
    26461 ggagaagaat tcagcgatgc tcgcggcaac cttggggccg acctcgggga cttcaacgag
    26521 ctgctcctcg gtggctgcgg cgagctcttc gagcgaagag aaatgttcgg cgaggagctg
    26581 tccggtgcgt tcgccgacaa agcgcatgcc gagcgcgtag atgagccgcg ccagtgaatt
    26641 cttcttactc gcttcgattt cgtcgaggag gttttgcgct gatttctccg ccatgcgctc
    26701 gagggcggcg agtgcgccga gtttcagcgc gtagagctcg gcgacatcct tcacgattcc
    26761 tttgtccacg agctggtcca cgattttttc gccgaggccg tcaatgttca tggcgtggcg
    26821 cccagcgtag tggagcagag actccttgcg cttggcgggg caggcggcgt tgacgcagcg
    26881 ataggcaacc tcgtcttcgg acttgtggat gcggctgccg cattcggggc attccctggg
    26941 catgcggaag gcgcggcgcg gcttcccttc cttgacgact ctcacaacgt gggggatgac
    27001 ttctccggcg cgctcgatca agacggtatc tccgatgtgc aggccgaggc gctcgatttc
    27061 gtccatgttg tggagggtgg aacggctgac agtgacgccg ccgacctgca ccggcttgag
    27121 gaccgcgaca ggggtgaggg cgccggtgcg gccgacctga acaaggatct cgttgacgac
    27181 cgtggtttcc tgctgcgcgg gatatttgta ggcgatggcc cagcgcggcg cccgggccgt
    27241 gtagcccagc tcgttctgaa tgccggtaga gttgaccttg acgacgacac cgtctatttc
    27301 ataggccagc ttctcacggc gggcgttcca ggcgtcgcaa taagcgagga cttcctcgat
    27361 ggaggggcag agtttccagt cgtcggaggc gcggaagcgc aattgcttga ggacttgcag
    27421 actttccgag tgtttttcaa acgggacttt gccgtccgcg aggaggtagt aggcgaagaa
    27481 gtcgagattg cggttggcgg tgatcgcggg gtcgaggacg cgcacggcgc cggcggcggc
    27541 gttacgcggg ttaacaaaga ttttgccgcc ctgcttttcc tgcgcgcggt tgagggcgtc
    27601 gaaggccttg cgggtcatca tcacttcgcc gcgcacctcg aaatcggcgc ggaggccggc
    27661 cttcttgagc gcggccgttt cgacgcggag aggaatcgat cgaatggtgc ggacgttgag
    27721 cgtcacgtct tcgccggtgg tgccgtcgcc gcgggtgacg ccgcggacga ggacgtcgtc
    27781 ttcgtattgg agagagatgc tcaggccgtc gaacttgtgc tcggcgatgt actcgattct
    27841 ttcccggccg ctgccctcac ggacgcggcg gtcccagtcg cgcagcgcgt cgtaggaaaa
    27901 ggcgttgtcc aggctgagca tggcgcgggc gtggcggacg gtctggacgc cttcgcgagg
    27961 cgcgccgccg acgcgctggc tgggcgaatc gggcgtgatg agcgagggat gctccgcttc
    28021 gagcaccttg aggcggctca cccggcggtc gaactccgcg tcggagatct gcggatcgtc
    28081 ctttacgaag tagaggtact cgtggcggcg aatctcttcg cggagctttt cgatttcctc
    28141 tcttgcagcg gcggctttga ccatgaaatc cccaaaaaac ctttccgaaa aggccgggcg
    28201 ttattatata gaatgaacgt ctgcgagaaa gaacagcttg ctttcctaga ccgatgaccg
    28261 ccgacctgca caacgccctg ttgatccaca acccccgcgc tggaagcggg ggccggcgcc
    28321 gcctgttgga tgaggcgcgg cgcatcctgg ccgcgggagg gattgagacc gagctggcgg
    28381 atacaggcgc gccagggcac gcgacggaac tagcgcagac cgctggggcc agcgggcgcg
    28441 gcctggtgat cgtgtgcggc ggggacggca cgctgaatga agtggtaaac ggactggcgg
    28501 cgagtccgaa cgggcatcgc gtgccgctag ccctgcttcc tgccggcacg gcaaatgtcc
    28561 tggccaagga actcggtctg ccgtggaaca ttccgcgcgc ggcggaaaag cttctgcgcg
    28621 ggaatttgcg ggagattgcc ctggggctgg cgacaccgct ggagaggccg gaaaagaagc
    28681 gctatttcgt cagcgtggcg ggggccgggc ccgacggagc gctggtgtat tcgctggatc
    28741 ttggactgaa ggcccgcatc ggggtcctcg cgtactgggg ggagggcgtg cggcaagtgg
    28801 tgcgttacaa atttccaaaa ttccggatca tcaccgcgga aaaacagctc gaagccacgc
    28861 tggccgttgt gggacggacg aagcattatg gcggcccgtt caagatcacg acgcgcgcgg
    28921 acctgctcga agaccgcttc gagcttgccg cgctcaccac gcggagcgcc ctgcgctacc
    28981 tgagttatct gccggcgcta tggttcggcg atctgcgcgc gagggaaggc gtgcacttct
    29041 ggaagtcgga gaagattgtg tgcgagccgc tggggaccga acccgtgtac gcgcaggtgg
    29101 atggcgagcc tttggcgcgt ctgccggtgg agttccggat tgtcccgcgg gcggtga
//
LOCUS       DASYVO010000554         5240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_195217, whole genome shotgun sequence.
ACCESSION   DASYVO010000554 DASYVO010000000
VERSION     DASYVO010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195217"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1709
                     /locus_tag="VGR03_19125"
     CDS             <1..1709
                     /locus_tag="VGR03_19125"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEV1996453.1"
                     /translation="GIFDNLPGYLKVPTPADGSFEGWTKRITPKPAKPGQWDSFNYWG
                     NTPKFAVSFLKAMYGEAATKQSDWAFDYLPKVDRNFSWVYLWDNMYNGIVKGIFAFGM
                     NGVAIGPDSKKNVEALKKADWLVVGEIYPDETSEFWKSPGISTDEMKKIQTTVYRLPC
                     AGFAEKDGTFTNSARWLQWKNAALPMPGDCRLDQAIVAQIFLKVRELYKKESGKFPDP
                     ILKLTWNYTDPSNPSLAEVLKEINGKTFVDAEDPVTKIQLKAGQQVPGFAWLKDDGTT
                     ACGNWIYCGSYTEAGNLTMRRDASDPSGLGIHQNWAWSWPANRRVLYNRASCDLDGKP
                     WDASRRQVWWSEATQKWAGNDVPDFKADSNPKDHMGPFIMNPEGVGRIFAPLGAFADG
                     PFPEHYEPIESPIDNPLHPEHTHNPVVKRFKTPEDKYGTPKDGYTVVCTTYRLTEHYH
                     YWTKNNPMNVQLVPEPFVEIAAEMADEMGIRGGETLKITSARGHYIAKAMVTKRIKAM
                     MIDGKKTYQIGIPIHWGYRGIAEDEGKTAKTLTNQLSPTVVDPNAYTPEFKGFLVKVE
                     KA"
     gene            1718..2611
                     /gene="fdxH"
                     /locus_tag="VGR03_19130"
     CDS             1718..2611
                     /gene="fdxH"
                     /locus_tag="VGR03_19130"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HEV1996454.1"
                     /translation="MSQATLRIQKISGHAGSVPGEDTQRGLTVCKLIDTTTCIGCKAC
                     EVACMEWNDLPFGETVFGNSYQTMPETRWNYWNLILFNEHERDDGTLMWLMRKNQCMH
                     CAEPGCLAACPADGAIVQYTNGIVDFQQANCIGCGYCITGCPFDIPKMNPKTNRVFKC
                     TLCSDRVSEGLEPACIKACPTGCLHFGTKEDMLDLAGKRAKQLQQHSNFPNAGVYDPK
                     GVGGTGVIYVLHDATKPEIYGGLPKDPHIPWMVKLWKGPLKWLGNVAMVGGLIGLFVH
                     YLRYGPKAREDEDINPGGAKT"
     gene            2608..3270
                     /locus_tag="VGR03_19135"
     CDS             2608..3270
                     /locus_tag="VGR03_19135"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEV1996455.1"
                     /translation="MSQSEQILPHGHVLRYNLRERLTHWVAAGAYIYLLMTGLAFWSP
                     WLFWVAVILGGAPVSRMLHPWAGLIFFAAVWKMYVMWAPQMKHTDVDRAWWKAVRYYS
                     TNQDDKMPPTGRYNAGQKFLFWGFFWSTVALLASGLVLWFPASIPWNLRLLRTISILV
                     HPAAALFTIGLFMIHLYMGLFAERGAFGSVIRGDVSIAFAKRYHPGWYEEITGESSTP
                     RK"
     gene            3267..4097
                     /locus_tag="VGR03_19140"
     CDS             3267..4097
                     /locus_tag="VGR03_19140"
                     /inference="COORDINATES: protein motif:HMM:NF016131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HEV1996456.1"
                     /translation="MTTIQTGGAEYDARIRRAERLSVQHLFAAEFLDFYKHVAAIQKT
                     LRANIASSNGIKSPSAPAAELRDPLDLTVVLPHFRGFLSSIEQHAPSALRKAAHQMSL
                     LPSDSWIASLEAYWKHGGKYDQQVGAFAQFLARAFLQPYAEFRVAQTPKAPIVVTTRV
                     CPLCGARPLLGVLRPEGDGGKRFLLCSFCSQEWEFRRILCPTCGEETENKLPVYIAEQ
                     LPHIRVEACDTCHFYIRTVDLTKDGHAIPLVDDLAAIPLTLWAHEKGYSRLQPNLLGT
                     "
     gene            complement(4205..4888)
                     /locus_tag="VGR03_19145"
     CDS             complement(4205..4888)
                     /locus_tag="VGR03_19145"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HEV1996457.1"
                     /translation="MTIDNVPLANEPATRGKPGSSQNLRSWVLGTFLGAVLTVVLIGA
                     LVWLSTGLGLLHLMGILRGGRTQINVDQPTVVRQIQQLQRLETVSYTMDKIISGEHAN
                     AYLPKFLAGDRLLLVVHGDVVAGINLAGLQPGDVLIQGQKVSIHLPAAEVFSTRIDNA
                     KTKVYSRDTGLFSSPDPNLESEVREEAERQLQQAALQDGILKTAADNARSTISGMLKG
                     FGFHEVDIR"
     gene            complement(5025..>5240)
                     /locus_tag="VGR03_19150"
     CDS             complement(5025..>5240)
                     /locus_tag="VGR03_19150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEV1996458.1"
                     /translation="TLGAIPVAGDIFHVFWKANRRNYRLLIREQEQPGANTRRDWMFL
                     AIILFTAIAAAAIPIGIMIWILWPLLG"
BASE COUNT         1163 a         1443 c         1584 g         1050 t
ORIGIN      
        1 ccggcatctt tgacaatttg cccggctatt tgaaagtgcc gacacccgcg gatggttcgt
       61 tcgaaggctg gaccaagcgc atcacgccga agccggcgaa acccgggcaa tgggattcct
      121 tcaactactg gggcaatacg ccgaagtttg ccgtgagttt cctcaaggcg atgtacggcg
      181 aggcggccac caagcagagc gattgggcgt tcgactattt gccgaaagtg gaccgcaact
      241 tttcgtgggt ctacctctgg gacaacatgt acaacggcat cgtcaaaggt attttcgcct
      301 ttgggatgaa cggagtagcg atcggtccgg attcgaaaaa gaacgtcgaa gctctgaaaa
      361 aagcggactg gctcgtcgtg ggagagattt atccggacga gacgagcgaa ttctggaagt
      421 caccgggaat ttccaccgac gaaatgaaga agattcagac gacggtctac cggctgccgt
      481 gcgccggatt cgcggaaaaa gacggaacgt tcacaaactc ggcgcgctgg ctgcagtgga
      541 aaaatgcggc gctgccgatg ccgggagatt gccggctgga tcaagccatc gtggcgcaga
      601 ttttcctgaa ggtgcgcgag ctctacaaga aggagagcgg aaaattccct gacccgattc
      661 tgaagctgac gtggaactac acggacccat caaatccatc gctggccgaa gtgctgaagg
      721 aaatcaatgg aaagaccttt gtggacgccg aggatccggt cacaaagata caactcaagg
      781 ctgggcagca ggtgccgggg tttgcctggt tgaaggacga cgggacgacg gcttgcggga
      841 actggattta ttgcgggtcg tacacggagg ccgggaatct gacgatgcgg cgcgacgcct
      901 cggatccgtc cgggctgggc attcaccaga attgggcgtg gtcgtggccg gcgaaccggc
      961 gggtgctcta caaccgcgcg tcgtgcgacc tggacggcaa gccatgggat gcgtcgcggc
     1021 ggcaagtgtg gtggagcgaa gcgacgcaga agtgggcggg caacgacgtt ccagacttca
     1081 aggcggactc caaccccaag gatcacatgg ggccgttcat tatgaacccg gagggcgtag
     1141 ggcgaatttt tgcgccgctc ggagcatttg cagacggacc tttccccgaa cattacgagc
     1201 cgattgaaag cccgatcgac aatccgctgc atccagagca tacacacaac ccggtggtga
     1261 aaagatttaa gacaccagag gacaagtacg ggacgccgaa ggacggctac acggtggtgt
     1321 gcacgaccta tcggttgacg gaacactacc actactggac gaagaacaat ccgatgaatg
     1381 tgcagctggt gccggagccg ttcgtggaga tcgccgccga aatggccgat gaaatgggaa
     1441 tccggggcgg agagacattg aagatcacca gcgcacgcgg gcactacatc gcgaaggcga
     1501 tggtcacgaa acgcatcaag gcgatgatga ttgacgggaa gaaaacctat cagatcggca
     1561 ttccgattca ctggggctac cgtggaattg cggaagacga aggcaaaacg gcgaagacac
     1621 taacgaacca gctttcgccg acggtcgtcg atcccaatgc ctacacgccg gaattcaaag
     1681 gattcctcgt gaaagttgag aaagcgtagg ggatcccatg agccaagcca cactacgcat
     1741 tcaaaaaatc tcaggacacg cggggagcgt tcccggcgaa gacacgcagc gcggactcac
     1801 ggtctgcaaa ctcattgaca ctacgacgtg catcggctgc aaggcatgcg aagtggcgtg
     1861 catggagtgg aacgatctgc cgttcggaga aacggttttc ggcaacagct accagacgat
     1921 gccggagacg cgctggaatt actggaacct gatcctcttc aacgagcatg agcgtgatga
     1981 cggcacgctg atgtggctga tgcgcaaaaa ccagtgcatg cactgcgcgg agccgggatg
     2041 cctggcggca tgtcccgcgg acggggccat cgtgcagtac acgaatggta tcgtggattt
     2101 tcagcaggcc aactgcatcg ggtgcggcta ttgcatcacg ggatgtccgt tcgacattcc
     2161 aaagatgaat ccgaagacca atcgcgtgtt caagtgcacg ctgtgttcag atcgcgtgag
     2221 tgaggggctc gaaccggcat gcatcaaggc gtgtccgacg ggctgcctgc acttcggcac
     2281 caaggaagac atgctggacc tcgcgggtaa gcgagcaaag caactgcagc agcattcaaa
     2341 cttcccgaac gccggcgtgt acgatccaaa aggtgttggc ggcaccggcg tgatttacgt
     2401 gctgcacgac gcgacgaagc cggagattta cggcggtctg ccgaaagatc cgcacatccc
     2461 gtggatggtg aaactgtgga aggggccgct gaagtggctt ggcaacgttg ccatggttgg
     2521 cggattgatc ggcctcttcg tgcactacct gcgctacggc ccgaaggctc gcgaggatga
     2581 ggacatcaat ccgggaggag caaaaacgtg agtcaatccg aacagatact tcctcacggc
     2641 catgtcctcc gctataactt gcgcgaacgg ctgacgcact gggtcgcggc gggagcatac
     2701 atttatctgc tgatgaccgg gctggcgttc tggtcgccgt ggctgttctg ggtggcggtg
     2761 attctgggcg gggcgccggt ttcgagaatg ctgcatccgt gggccgggct catttttttt
     2821 gcggcggtgt ggaagatgta cgtgatgtgg gcgccgcaaa tgaagcacac cgatgtggac
     2881 agagcgtggt ggaaagcggt ccgctactac agcacgaatc aagacgacaa gatgccgccg
     2941 acgggacggt acaacgcggg gcaaaaattt cttttctggg gatttttctg gagcacggtg
     3001 gcattgctgg cgagcggcct cgtgctgtgg ttcccggcgt ccattccgtg gaatcttcgc
     3061 ctcttgcgaa ccatttccat tctcgttcat cccgcggcgg cgcttttcac gattggcctg
     3121 ttcatgatcc acctgtacat gggtctgttt gccgaacgcg gagcatttgg ctcggtcatt
     3181 cgcggtgatg tttcgatcgc ctttgccaag cgctatcacc ccggctggta tgaagagatc
     3241 accggcgaat cgtccacgcc gcgcaaatga cgacaatcca gaccggtggg gccgagtacg
     3301 acgcgcgaat acggcgcgcc gagcggttat ccgtgcaaca cttgttcgcc gctgaatttc
     3361 tcgatttcta caagcacgtc gcggcgattc aaaaaacgct tcgcgcaaac atcgcgtcct
     3421 cgaatggcat aaaatcgccc agcgcgcccg cggcggagct gcgagatcca ctggacctca
     3481 ctgtggttct cccccacttc cggggctttc tctcctcgat cgagcagcac gctccttcgg
     3541 cgctaaggaa agccgcgcat cagatgtcct tgctgccatc agattcctgg attgcgagcc
     3601 tggaagcgta ctggaaacat ggcggcaagt acgaccagca agtcggagca ttcgcacagt
     3661 tccttgctcg cgcgttcctg caaccgtatg ccgagtttcg cgtagcgcag acaccgaagg
     3721 ctcccattgt agtgaccacg cgcgtttgtc ccttgtgcgg ggcgcggccg ctcttgggcg
     3781 tgctgcggcc ggaaggcgat ggcgggaaac gatttctgct ctgttcgttt tgttcgcaag
     3841 agtgggaatt tcggcggatt ttgtgcccga cctgcggcga agaaaccgaa aacaagttgc
     3901 cggtgtacat cgccgaacag ttgccacaca ttcgcgtgga agcctgcgat acctgccact
     3961 tttatatccg cacggtcgac ctcacgaaag acggccacgc catcccgctg gtcgatgatt
     4021 tggcggcaat cccgcttacg ctctgggccc acgagaaggg gtattcgagg ctccagccga
     4081 acctactggg gacctgaagt gcgttcgggg tggctcgtgt tccgtgaacc cggcttatca
     4141 gaattggccg ctatcataat gcgtttcgag cgcggatgct gtttgtaaaa tccttgccgt
     4201 gaacctaccg gatatccact tcgtggaagc cgaagccctt tagcatgccc gagatggtgc
     4261 ttcgcgcgtt gtcggcagcg gtcttgagga tgccatcttg cagcgcggct tgctggagct
     4321 ggcgctcagc ttcttcgcgg acttcgctct caaggttggg gtctggggaa gagaataggc
     4381 cggtgtcccg ggaatatact ttcgtcttcg cattatcgat gcgcgtgctg aagacttccg
     4441 cggctggcaa gtgaatcgaa actttctgtc cttgaattag gacatcgccg ggctgcaaac
     4501 ccgcgagatt gatccccgca accacgtctc cgtgcaccac cagtagcagg cggtcacccg
     4561 ccagaaattt cggcaggtaa gcattggcgt gctcgcccga aataatctta tccatcgtgt
     4621 agctcacggt ttccagccgt tggagttgct gaatttgacg aaccacggtt ggctgatcca
     4681 cattgatctg cgtgcgtcct ccgcgcaaaa tgcccatcag gtgcaataac ccgagcccgg
     4741 tcgacagcca cacgagcgcc ccgatcaaaa caacagtaag aaccgcgccc aagaaggtcc
     4801 ccagaaccca ggagcgcaag ttctgggagc ttcctggctt tccgcgagtt gccggttcgt
     4861 tagcgagagg tacattgtcg atcgtcattt gtttcctcaa cgacttcagt gtagaccagc
     4921 caaaaaacat tgcccgcatt tgtccccaac gaccgcctag gcgtcgctcc ctaaactgct
     4981 gcccttcgga ggctcgtttc tgcaaggccg caggcgcgga aactctagcc taaaagaggc
     5041 cacagaatcc aaatcataat gccaatcgga atcgccgccg cagcaatagc tgtgaatagg
     5101 atgatcgcga ggaacatcca atcccgcctg gtgttggcgc cgggctgctc ctgctctcgg
     5161 atcagcagcc ggtagttccg tcgattcgcc ttccaaaaga cgtggaagat gtcgcctgct
     5221 accggtattg ctcctagtgt
//
LOCUS       DASYVO010000555         2978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_195301, whole genome shotgun sequence.
ACCESSION   DASYVO010000555 DASYVO010000000
VERSION     DASYVO010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195301"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VGR03_19155"
     CDS             <1..831
                     /locus_tag="VGR03_19155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996459.1"
                     /translation="GWSSSPSAPRPGLAGIPGPPRQRYALRHRLSVASRYGASPHSRC
                     AGGIHENRFVIFLAAALVCTAAPSSYAEPRQVIQGTQVHLTLLGGISSAIAKDGDPFV
                     AIVAEPVCLGSQLLLPAGTRVNGLIGTVEKARHFSIFRGQAYMNLTFRSIEVDSRLIP
                     VQMSIITIEQPHRQADGKRRKDVKIAEGQVVQEKHDVKGDIVGATIGTGGGTLIGAVF
                     SHVARGLGFGLAGSAACIVARKGKDLDLPAQTGMLIRMDNTITVPSTSAASTGYSGNR
                     "
     gene            complement(938..1552)
                     /locus_tag="VGR03_19160"
     CDS             complement(938..1552)
                     /locus_tag="VGR03_19160"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV1996460.1"
                     /translation="MRTKSLAGVLIALLLMAGAAAASAQEVTQAEKDHALQYLETTKK
                     NVLEATKGLSEAQWNFKPAPERWSVAQVMEHIAAAEDFIRGMVKEKVMMAPAGEPGRD
                     VKKTDDGVLAMVPDRTTKVQAPEPLVPTNRFGSAEGSIKHFLESRATTEDFLKTTTGL
                     RDHVLDSPMGKLDGYEFVLLIAAHSERHTKQINEVKVDPNFPKK"
     gene            complement(1614..2435)
                     /locus_tag="VGR03_19165"
     CDS             complement(1614..2435)
                     /locus_tag="VGR03_19165"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1996461.1"
                     /translation="MSNQKRYIPEGFFSLTPYLVCNEAARLLEFLKRAFGAEETFRMN
                     RKDGTIGHASARIADSMLEMADPAEEWKAMLAGLHLYVPNVDEVYQRAVQAGARSLYE
                     PRDMDYGDREGGIQDPSGNHWYIATHKAGKHFAPEGLRSVTPGMSVKDAAKFLQFLVK
                     AFEANVVQKNETTDGTVGHAKVRIGDSILECSEAHGEWGPRTATMHLYVPDVDAVYER
                     ALKAGATSLSEPKDQFYGERSGGVSDEWGNNWYVATHKENLTKEEVEKRASAQGG"
     gene            complement(2574..>2978)
                     /locus_tag="VGR03_19170"
     CDS             complement(2574..>2978)
                     /locus_tag="VGR03_19170"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1996462.1"
                     /translation="KLRRLEETLLSIGRGQFERHPAVAFALGEFQAAPQGQKIVEVTG
                     RTGFSARRFIDLFEKEVGLTPKLFCRVQRFQRVLHMIQTCCSIDWAEIALSCGYYDQA
                     HFIHDFRTFSGINPSTYVAARTPHLNHVPILD"
BASE COUNT          535 a          977 c          780 g          686 t
ORIGIN      
        1 ggatggagtt cctccccatc cgccccgcgt cccggcttgg ccgggatacc gggtccgccg
       61 cgccagcggt acgcactgag gcatcgcctc agtgtcgcct cacgatacgg ggccagcccg
      121 cactctcggt gcgcaggagg aatccatgaa aatcgctttg tcattttttt ggcggcagct
      181 ctcgtctgca ccgccgcgcc ttcgtcttac gccgaaccgc gtcaggtcat ccaggggacg
      241 caggttcatc tcacgctctt gggcggaatc agcagtgcca tagcaaagga tggcgaccca
      301 ttcgtcgcca ttgtcgccga gcccgtctgc ctcggcagcc agttgttgct ccccgccggt
      361 acgcgtgtga acggcctcat tggcactgtg gagaaggccc gccatttttc catctttcgc
      421 ggccaggctt acatgaacct cacgttccgc tccatcgaag tcgacagccg cctcatcccc
      481 gtgcagatga gcatcattac catcgagcaa ccgcatcggc aggccgacgg caagcgccgc
      541 aaagatgtga aaattgcaga aggccaggtc gttcaggaaa agcatgacgt caagggcgac
      601 atcgtcggcg cgaccatcgg gaccggcggc ggcactctca tcggtgcggt ctttagccac
      661 gtcgcgcgcg gcctcggctt cggcctggcc ggcagcgccg cttgcattgt cgcgcgcaag
      721 ggcaaggacc tcgatcttcc cgcgcaaacc ggaatgctga ttcgcatgga caacaccatc
      781 accgtgccgt cgaccagcgc cgccagcacc ggctattccg gcaaccgctg agactcgcca
      841 agaattaaga tgcgccgcga agggacactc caacgtgtcc cccgcggcgc atgatttctc
      901 tgcgaactcc gtgtcctctg tgttagatcc ttttccctta tttcttcgga aagtttggat
      961 ccaccttcac ttcattgatc tgcttggtgt gccgttcgct gtgtgctgcg atgagcaaaa
     1021 caaactcgta cccatccagc tttcccatcg ggctgtccaa cacgtgatcg cgcagacccg
     1081 tggtcgtttt cagaaaatcc tccgtcgtcg cgcggctctc taggaaatgc ttgatcgagc
     1141 cttctgccga accgaaacgg tttgttggca ccagcggttc cggcgcttgc actttggttg
     1201 tgcggtcggg aaccatcgcc agcactccgt cgtcggtttt cttcacgtcg cggcccggct
     1261 cccccgccgg cgccatcatg accttttcct tcaccattcc gcggataaag tcctccgccg
     1321 ccgcgatgtg ctccatcacc tgcgccactg accagcgctc cggcgccggc ttgaagttcc
     1381 attgcgcttc cgagagcccc ttcgttgctt cgaggacatt cttctttgtt gtttccagat
     1441 actgcagcgc gtgatccttc tccgcctgcg tcacctcttg tgccgatgcc gccgctgctc
     1501 ccgccatcaa aagcaacgcg atcaacaccc ctgccaggga cttggttctc atttttcttc
     1561 tctccttatt ttggtctttg ggtggagaaa caggaaccaa gagcatacca gatttaccct
     1621 ccttgcgccg atgcccgctt ctcgacttct tccttggtca agttctcctt gtgtgtggca
     1681 acgtaccagt tgttccccca ctcgtccgac accccgccgc tgcgctcccc atagaattga
     1741 tccttcggtt ctgacaaaga agtcgctccg gccttcagcg cccgttcgta caccgcgtcc
     1801 acgtccggca cgtagagatg catcgtcgcc gtgcgcgggc cccattcgcc gtgcgcctcg
     1861 ctacactcca aaatcgagtc gccaatccgc actttggcgt gccccacggt gccgtccgtc
     1921 gtctcattct tctgtacaac gtttgcctca aatgctttaa ccaggaactg cagaaatttc
     1981 gccgcgtctt tcacgctcat ccccggcgtc acgctgcgca aaccctccgg cgcgaaatgc
     2041 ttcccggctt tatgcgtcgc gatgtaccaa tgattgcccg aaggatcctg gatgcctcct
     2101 tcacggtcac cgtagtccat gtctctcggt tcgtacaagg agcgcgctcc cgcttgcact
     2161 gcccgctggt acacttcatc cacattcggc acgtaaagat gaagtccagc cagcatcgcc
     2221 ttccattctt cggccgggtc ggccatctcc agcatggaat cggcgatgcg cgcggacgcg
     2281 tgcccaatcg tgccgtcctt ccggttcatt cgaaatgtct cctccgcccc gaacgcccgc
     2341 ttgaggaatt ccagcaagcg tgccgcctcg ttgcacacca agtacggcgt gagcgaaaag
     2401 aatccttcgg ggatgtatct cttttggttg ctcatgttga cctcgtttgg atcgatgaaa
     2461 tgttggatcg tccttcgtcg acgatattgt caaaagggcg gggaatcgta ttgtaaaaaa
     2521 ttgaaggcac gctgagcccg ccttcacgct aacttattgt gctctttcct cggtcaatcc
     2581 aggatcggca cgtggttcaa gtgtggtgtg cgtgcagcga cgtaggtgct cggattaatc
     2641 ccggaaaacg ttcgaaaatc gtgaatgaag tgggcttggt cgtaataccc gcaggagagc
     2701 gcgatctccg cccaatcgat ggagcagcag gtttggatca tgtgcagcac gcgctggaat
     2761 cgctgcacgc ggcagaagag cttcggcgtc aatcctactt ctttttcaaa caagtcgatg
     2821 aaccgcctcg cgctgaagcc cgtgcggccc gtcacctcca cgatcttctg tccttgcggc
     2881 gcggcttgaa attcgcccag cgcgaaggct accgcgggat ggcgttcgaa ctgtcctcgc
     2941 ccgatcgaca gcaatgtctc ttcgagtctt cgcagctt
//
LOCUS       DASYVO010000556         8226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_239406, whole genome shotgun sequence.
ACCESSION   DASYVO010000556 DASYVO010000000
VERSION     DASYVO010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8226
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239406"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..814
                     /locus_tag="VGR03_19175"
     CDS             347..814
                     /locus_tag="VGR03_19175"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV1996463.1"
                     /translation="MSPDFMRRAIVLALENVRSGSGGPFATVIVKEDRIIAEGTNSVT
                     LANDPTAHAEVVAIREACRVLADFQLSGCDLYTTCEPCPMCLGAIYWARPTRIFYAAT
                     AIDAASAGFDDAFIYDELKIAPAGRRIPMTQLLRDDSLAIFSAWKQQGNKTPY"
     gene            complement(859..1386)
                     /locus_tag="VGR03_19180"
     CDS             complement(859..1386)
                     /locus_tag="VGR03_19180"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV1996464.1"
                     /translation="MKKKNKRTTKKKSKAGNHDQALREHLVSLLKGGEAHVHFMDAIE
                     GFPEAKRGAFVEGLRHTGWQLMEHVRIAQWDILEFSRGAKHVSPGFPEGYWPKTPLPP
                     DEAAWAKSVQGFQHDLQEMIKLVKNAKTDLYAAIPHGEGQTILREALLLADHNAYHLG
                     QLVDLRRALGAWPEP"
     gene            complement(1396..2418)
                     /locus_tag="VGR03_19185"
     CDS             complement(1396..2418)
                     /locus_tag="VGR03_19185"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1996465.1"
                     /translation="MATPTATSRPTPEHIFNTLCAFQQTAALKAGIELDIFTAIADGE
                     NTPVLLAAKSGAAERGVRMLCDYLTIQGFLTKEAERYALTQESAIFLNRHSPACLASM
                     ANFLGSPWHKKNFESLAEAVRKGGTASEVGDNTKPNDEIWINFARSMAPLVIPAAEFI
                     AGLTGAAEGKPYKVLDIAAGHGMFGVTVARRNPHAQVVALDWPGVLEVARENASKFGV
                     ADRFTTLPGSAFETQLGNGYDFVLLTNSFHHFDMATCEKLMRRVHAALKPGGKAITLE
                     FVPNEDRVTPPTSAAFSLVMLAGTDSGDAYTFSQYEKMFGNTGFLKTTQHSVPDSPEQ
                     LLLSEK"
     gene            2566..3294
                     /locus_tag="VGR03_19190"
     CDS             2566..3294
                     /locus_tag="VGR03_19190"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEV1996466.1"
                     /translation="MKREYHRWYSHRLGMELGVAVFGHWGPPLLGFPTSAGDEWELEG
                     QSMVGALADFIEAGRIKLFTVNSINGHSFYNKSAHPFHRSYVQTVFDSYLREEVVPFI
                     WNNCQSQVAISTMGASFGAYHAANSLFKHPDVIKRCFAMSGVYDLRNFMDGMYDDNFY
                     FNNPVDYISNASDPWFYQQIASCDIHISTGTGPWENSGPTYRLSEVLSGKGIRHSLDN
                     WGPQGGHDWPHWKHQMREYISQLF"
     gene            complement(3336..4361)
                     /locus_tag="VGR03_19195"
     CDS             complement(3336..4361)
                     /locus_tag="VGR03_19195"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV1996467.1"
                     /translation="MNFGSPQADLMPGARNAVETCLGVRPGEHVALIADEASRAVAAS
                     IAGALDDRRAPYTGLLLEDFGPRPMKAAPADVLDALETADVGVMCMTPQPGELGARMA
                     IVRVVERRQIRYAHMVGVTPQIMRQGMRTDYRMVDRLSDSLRERMLRAETLTVITEAG
                     TKIAAHFDRGLDWVKTSGLISPRYWSNLPAGEVFTTPAMVDGTFVCDATAGDHFNGKY
                     GDLQATPLTLEIKGARLVGVDCARKDLEQEFWEYCHTDENSDRVGELAFGTNLGLSEM
                     IGVLLQDEKFPGVHIAFGDPYGSQTHADWNSKTHVDVLTRKCDVWIDDDQVIEKGQYQ
                     LEHLGLR"
     gene            complement(4400..4636)
                     /locus_tag="VGR03_19200"
     CDS             complement(4400..4636)
                     /locus_tag="VGR03_19200"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV1996468.1"
                     /translation="MTHYTYPITIEKDGKQYYAYSDDLPGVYGLGPTIEKAKTSILEA
                     IRLYILECRKKVNEFPLRGRLAKRPVEKSRLTRS"
     gene            complement(4677..6086)
                     /locus_tag="VGR03_19205"
     CDS             complement(4677..6086)
                     /locus_tag="VGR03_19205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996469.1"
                     /translation="MTSAPTPSVLPPSPAEDWHAILKTTLLRVPALPEDLFKRMRGAR
                     LTFGDRVHCPFLRPFFLSSEDEERVRTVAETIADLSERIVNTALDDKHLFAQLHLREE
                     EERLARIPTGYGRASTASRLDAFLLPDSLKFAEYNGESPAGAGYAETLAEIFRELPVM
                     GKFAQLYEVHSYPLSAKLLDALVMSYLDWGGSSTKPQIAIVDWEDVPTVSEFEILQER
                     FEKMGIPTLIADPRELEWDGKSLVAQDKKIDLVYRRVLINDIVAKPAECSALVKAYSA
                     NAVCVANNFRCKIPHVKAFFAVLTDEQNGGLFSHGERELIRKHIPWTRVVADVETAHY
                     GQPIDLLAFIRKERENLVLKPSDEYGGSGVTLGWETSEAAWDAAIELALSAKNGVWIV
                     QERIPIRREVFPYIADVGKVDYRDMLVDFAPYLFRGKLCGFLTRLSATGLANVTSGGG
                     QVPAFRVSPRDHGATHGHS"
     gene            complement(6091..7116)
                     /locus_tag="VGR03_19210"
     CDS             complement(6091..7116)
                     /locus_tag="VGR03_19210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996470.1"
                     /translation="MKTVGLLCGREYSFPPAFIERVKTLGAKDGVTAEFVKLGGTRMG
                     EPSRYSVIVDRISHEVEYYRGYLKHAVLQGTYVINNPFWWTADDKYFNYAVAAKLGIA
                     IPKTVLLPQKGYPSDMDITGESLHNLEYPVDWDGLLDYVGRPAILKPFSGGGWKHVYK
                     VHNKEELLAAYDKTSPYCMTLQEFIDFNQYVRCFTFGKTDIVPVHYDPKERRYLVDHQ
                     YLTPELGARVVKDAQIINQALGYEMNTIEFAVKDGVPYAIDFLNPAPDFERDRITEFY
                     FEHVVEKMARLVIDRALNGKASHPWPRWEEMLGIGAVAGFTGGPKSAAAGQKSAAAPV
                     SRSAQGS"
     gene            complement(7167..>8226)
                     /locus_tag="VGR03_19215"
     CDS             complement(7167..>8226)
                     /locus_tag="VGR03_19215"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEV1996471.1"
                     /translation="VSQLVSAASPDIAEQVKHELHQSIVETGTRICENVSELRIEMHR
                     TRGELVAAAERAGLQVAAAGTHPFSSWIDQVISPGERYQHIIEEMGQLARSLLIFGMH
                     VHIAMPDKQTTIDMMNGVRYFLPHLLALSTSSPFWMGRNTGLKSFRTTVFRRFPRTGV
                     PEQFESWSAYENFVNLLVKLNCIDNGKKIWWDVRPHPTFGTLEFRMFDVSTRVEEAVA
                     IAALTQAIIVKLHRLYQGNVSWRLYRRALIEENKWRAARYGIDGKLIDFGKEAEVPMR
                     QLVPELLELVDDVVDGLGSRSAVEYVHTIMNEGTSADRQLRVYKETGDLKAVVRHLVA
                     ETRGGVTAPRNSSVHAVN"
BASE COUNT         1617 a         2485 c         2324 g         1800 t
ORIGIN      
        1 tgcagcaaac atctgctccg gcatcaggcg aatgctcaga acaaatgctc ccgctccgag
       61 cacgccttta agaaatcccc gccggccgct gagcaactca cgatcgcggt tggaaatatt
      121 atcgatttga atcatgcgcc ctcgctccgc cactggacga aaatggatgc caaaccatct
      181 actgccacgc tacatcctac cgccaattcg ttttcttgca agcccggcgg ccgagaaaca
      241 tgccctgccg attgcccagg agttaagtga ttccacgcgt cgaaaaaatg cgcgttgcga
      301 ttctgagctg gaaagtacaa tctgaaacgc ggtagcagaa caagtcatgt ccccagactt
      361 catgcggcgc gccatcgtgc tggcgctgga aaatgtccgc tcgggcagcg gcgggccgtt
      421 cgctaccgtg atcgttaaag aagaccgcat cattgctgaa ggcacgaaca gcgtcactct
      481 tgcgaacgac ccgacggcgc atgcggaagt cgtggccatt cgcgaggcct gccgcgttct
      541 cgctgatttc caactcagcg gctgcgatct ctacacgaca tgcgagcctt gccccatgtg
      601 cctcggcgcg atttactggg cgcgccccac cagaattttc tacgcggcaa ccgcgatcga
      661 tgccgccagc gccggatttg acgacgcctt tatctatgac gagctgaaga tcgcaccagc
      721 gggccggcgc attccgatga cgcagcttct ccgcgacgat tccctcgcta ttttttcggc
      781 ctggaagcaa cagggaaaca aaacgcccta ctgacgcgaa ttctcggtct tccagcctca
      841 cccgcgattc acaagtgctt atggttccgg ccaggcgccc agcgcacgcc gcaaatcgac
      901 caactgaccc aggtgatagg cgttgtgatc ggccagtaga agcgcctcgc ggagaatcgt
      961 ctggccctct ccgtgcggaa tcgccgcgta caaatcggtt ttcgcgtttt ttaccagctt
     1021 gatcatttcc tggagatcgt gctggaaccc ttgcacgctc ttggcccacg cagcttcatc
     1081 tggtggtaag ggcgtcttcg gccagtagcc ctcgggaaat cccggtgaga cgtgctttgc
     1141 gccgcggctg aattccagaa tgtcccattg cgcaatccgc acgtgctcca tcaactgcca
     1201 tcccgtatgc cgcaagcctt cgacgaatgc tcctcgtttc gcttcgggga aaccctcgat
     1261 cgcgtccatg aagtgtacgt gcgcctcgcc acccttcaag agggatacga ggtgctcgcg
     1321 caacgcctga tcgtgatttc ccgctttgct tttctttttc gtggtccgtt tgtttttttt
     1381 cttcacgacg gtttcctatt tctccgagag aagcagttgc tctggcgagt ccggaaccga
     1441 atgctgcgtc gttttcagga acccggtatt cccgaacatc ttctcatatt gcgagaaggt
     1501 gtaggcatcg ccggagtccg ttccggctag catcaccagg ctgaatgccg cagacgtcgg
     1561 cggcgtgacg cgatcttcat tgggcacaaa ctcaagtgtg atagcttttc caccgggttt
     1621 cagcgccgca tggacgcgcc gcatcaattt ttcgcaggtc gccatgtcga agtgatgaaa
     1681 actgttcgta agcagcacga aatcgtagcc gttaccaagc tgcgtctcga acgcgctgcc
     1741 gggtagcgtt gtgaagcggt ccgcaacgcc aaatttgctg gcattctccc gcgccacctc
     1801 cagtacgcct ggccagtcca gcgccacgac ctgcgcatgg ggattacgcc tcgccactgt
     1861 gactccaaac atcccgtgtc ccgccgcgat atccagaacc ttgtagggct tcccctcggc
     1921 agcacccgtc aatcccgcga tgaattcggc agccggaatc accagcggcg ccatcgagcg
     1981 cgcgaagttg atccaaattt cgtcgttggg cttggtgttg tctccaacct cgctggcggt
     2041 gccgcccttc cgcacagctt ccgccaagga ttcaaagttt ttcttgtgcc acggactccc
     2101 aaggaaattc gccatcgagg ccaggcatgc cggagaatgg cgattcagaa aaatggccga
     2161 ttcctgagtg agcgcgtaac gctccgcctc cttggtcaag aatccctgga tggtcaggta
     2221 atcgcaaagc atccgcacgc cgcgttctgc agcgccgctc ttcgcggcaa gaagaacggg
     2281 ggtgttctcg ccgtccgcga tggcggtgaa aatatccaat tcgattcccg ctttcagcgc
     2341 ggcggtttgt tggaaggcgc agagtgtatt gaaaatgtgc tccggcgtag gccggctcgt
     2401 tgccgtgggg gtagccatga ataaactctc ctgtcctgcg atgtggacat cacaaattat
     2461 atacgcagaa cctcccaaag cgtttctttc tgcgggcaaa ccgattgccc gcagcccgcc
     2521 cggctggcat actttcttct ccccgcaaca gccaagaggc aatcgatgaa acgtgaatat
     2581 caccgctggt actctcaccg gctcggcatg gagctgggcg tcgccgtctt cggccactgg
     2641 ggcccgccgc tcctgggttt cccgacgagt gccggcgacg agtgggagct cgagggccag
     2701 agcatggtcg gcgcgctcgc ggatttcatc gaggccggta gaatcaaact cttcaccgtg
     2761 aattccatca acggacacag cttttacaac aagagtgcgc acccgtttca ccgcagctac
     2821 gttcagaccg ttttcgattc ctatctacgc gaagaagttg ttccctttat ctggaacaac
     2881 tgccagtcgc aggtcgccat ttccaccatg ggcgcttcct tcggcgcata tcatgccgcg
     2941 aactcgctct tcaaacatcc ggacgtcatc aaacgctgct tcgccatgtc cggcgtctac
     3001 gacctgcgga atttcatgga tgggatgtac gacgacaatt tttatttcaa caatcccgtc
     3061 gactatatct cgaacgccag cgatccctgg ttctatcagc aaatcgcaag ttgtgacatt
     3121 cacatcagca ccggtacggg tccatgggag aacagcgggc ccacttaccg cctctctgaa
     3181 gttctctccg gcaaaggcat ccgccattcg ctggacaact ggggcccaca gggcggccac
     3241 gactggcccc attggaaaca ccagatgcgc gaatatatct ctcagctctt ttaactaatt
     3301 gctgatgcta acgttttgcg gtttgggggt ggctttcagc gaaggcccaa atgctcgagc
     3361 tggtactggc ctttttcgat gacttggtcg tcgtcgatcc atacgtcgca cttgcgagta
     3421 agcacgtcga cgtgggtttt agagttccag tcggcgtgcg tctggctgcc gtagggatcg
     3481 ccgaaggcga tatgcacacc cgggaatttt tcatcctgaa gcagaacccc gatcatctcg
     3541 gatagcccaa ggttggtgcc gaatgcgagc tcgccgacgc ggtcgctgtt ctcgtcggtg
     3601 tggcagtact cccagaattc ctgctccaaa tctttccggg cacagtcgac gcccacgagc
     3661 cgcgctccct tgatctccag cgtcaacggc gtggcttgca ggtcgccgta ttttccgttg
     3721 aagtgatcgc cggctgtggc gtcgcagacg aaagtaccgt ccaccattgc gggtgtagtg
     3781 aaaacttcgc ccgccggaag gttcgaccag tagcgcgggc tgatgagccc gctggtcttc
     3841 acccagtcga ggccgcggtc gaaatgcgca gcgatcttcg tgccggcttc ggttattaca
     3901 gtgagggttt cggcgcgcag catgcgttca cgcaggctgt cgctcaagcg atccaccatg
     3961 cggtagtccg tgcgcatgcc ctgacgcatg atctgcggtg tgacgccgac catgtgcgcg
     4021 taacgaatct gccggcgctc gaccactctc acgatggcca tgcgcgcacc cagttcgccc
     4081 ggctgtggcg tcatgcacat cacgccgaca tccgcggttt ccaaggcgtc cagcacatct
     4141 gctggcgcgg ccttcattgg ccgtggtcca aaatcttcca gcaacaatcc ggtataaggc
     4201 gcgcgccgat cgtccagcgc gccggcgata ctggccgcga cagcacggct ggcttcatcg
     4261 gcaatcagcg ccacgtgttc cccgggtcgc acaccaagac acgtttccac ggcgttccgc
     4321 gcgccaggca tcaagtccgc ttgtggggaa ccaaagttca tcctaataat tctacttgaa
     4381 ttggcgggat aatctagcgt cagctccggg tgagtctact cttttcaact ggcctcttgg
     4441 ccagtcgtcc gcgcagagga aattcgttta ctttttttcg gcactccaaa atatacagcc
     4501 gtattgcttc gaggatgctg gttttcgcct tttcaatcgt aggcccgagc ccgtagacgc
     4561 ccggcagatc atcactgtaa gcgtaatact gcttgccatc cttttcgatg gttatcggat
     4621 aagtgtagtg ggtcattgca attcgatcgt agccccgaag acgcggtcag gcaagctcat
     4681 gaatgtccgt gggtggctcc atggtcacgc ggggacacgc gaaacgccgg cacctggccc
     4741 ccgccgctgg tcacattggc gaggccggtg gcgctgaggc gggtgagaaa gccacagagt
     4801 tttccgcgga acaagtacgg ggcgaaatcc accagcatgt cgcggtaatc gaccttgccg
     4861 acgtccgcga tgtagggaaa gacttcgcga cggatgggga tgcgttcctg gacgatccac
     4921 acgccgttct tcgccgaaag cgcaagctcg atcgccgcat cccaggcggc ttcgctggtt
     4981 tcccagccca gggtcacgcc ggagccgcca tactcatcac tgggcttaag gaccaggttt
     5041 tcgcgttctt tacggatgaa ggcgagcaaa tcgatgggct ggccgtaatg cgccgtctcg
     5101 acgtccgcga caacgcgtgt ccacggaata tgcttgcgga tcagttcgcg ctcgccgtgg
     5161 gagaatagcc cgccgttttg ctcgtccgtc agcacggcga aaaacgcctt cacatgcgga
     5221 atcttgcagc ggaagttatt cgccacacac acggcgttcg ccgagtaggc tttgacgagg
     5281 gccgaacact cggcaggctt ggccacgatg tcgttaatga gcacgcggcg gtagacgaga
     5341 tcgatcttct tgtcctgcgc gacgagcgat ttgccgtccc actcaagttc gcgtgggtcg
     5401 gcgatgagcg ttgggatgcc cattttctcg aagcgctcct gcaaaatttc aaactcgctc
     5461 acggtcggca cgtcttccca gtccacgatg gcaatctgcg gcttcgtgga cgatcctccc
     5521 cagtccaggt agctcatcac cagcgcatcg agcagcttgg cgctgagcgg gtaactgtga
     5581 acttcataca gctgcgcaaa tttgcccatc accgggagtt cgcggaagat ctccgcaagc
     5641 gtctccgcgt agcccgcacc cgccggcgat tcgccgttgt attccgcaaa tttcagcgag
     5701 tccggaagta gaaacgcatc caggcgcgag gccgtgctcg cccgtccata tcccgtggga
     5761 atccgagcga gacgctcctc ttcctcccgc agatgcaatt gtgcaaaaag atgtttgtcg
     5821 tcgagcgcgg tgtttacgat gcgctcgctg agatccgcga tggtttccgc aacggttctt
     5881 actcgctctt cgtcttcaga agaaagaaaa aaggggcgca ggaaaggaca gtgaacgcgg
     5941 tcgccgaagg tgagcctggc tccgcgcatg cgcttgaaca aatcttccgg gagcgccggc
     6001 acacgcaaaa gcgtcgtttt caatatggcg tgccagtcct cggccgggga tggaggcagt
     6061 accgaaggtg tcggagcgct ggtcatttca tcacgatccc tgtgcggacc tggaaaccgg
     6121 agcggccgcc gatttttgtc cggcagcggc gctctttgga cctcccgtaa accccgcaac
     6181 tgcgccgatg ccaagcattt cttcccagcg cggccaggga tgcgaagcct tgccattcag
     6241 ggcgcggtca atcaccagcc gcgccatttt ttccaccacg tgctcgaaat agaattccgt
     6301 gatgcggtct cgctcgaaat ccggcgccgg attcaggaag tcgatggcgt agggcacccc
     6361 gtctttaacg gcgaactcga tggtgttcat ttcgtagcct agcgcctggt tgatgatctg
     6421 cgcatctttc acgacgcgcg cgccaagctc cggcgtcagg tattggtggt ccacaaggta
     6481 tctgcgttcc ttgggatcgt agtgaacggg gacaatgtcc gtcttgccga aagtgaagca
     6541 gcgcacgtac tgattgaaat cgatgaattc ctgaagcgtc atgcagtaag gcgaagtctt
     6601 gtcatacgcg gcgaggagtt cctctttgtt atgcactttg tagacgtgtt tccagccacc
     6661 acccgaaaaa ggcttcagga tggcgggtcg ccctacgtaa tcgagcagtc cgtcccagtc
     6721 caccggatat tccaggttgt ggagcgactc gccggtgata tccatatcgc tgggataacc
     6781 cttctgcggg agaagcaccg tcttgggaat ggcgatgccc agctttgcgg ccacggcgta
     6841 gttgaaatac ttgtcgtcgg cggtccacca gaacggattg ttgatgacgt aggttccctg
     6901 aagaaccgcg tgcttcaggt acccgcggta atattcgact tcatgcgaga tgcggtcgac
     6961 gatgacgcta tatcgtgagg gttcgcccat ccgtgtgccc cccagcttca caaattcggc
     7021 ggtcactccg tcctttgcgc cgagcgtctt gacgcgctcg ataaaagccg gaggaaagct
     7081 gtattcccgc ccgcagagaa ggccaaccgt tttcatgttc gtgctcctgt cagggtcctg
     7141 tggaactgtc tcgcttgcga tcgccgtcaa ttcacagcgt gtactgagga attgcgcggc
     7201 gcggtaacgc ctccgcgcgt ttcggctaca aggtgccgga ccaccgcttt caagtcgccg
     7261 gtctccttgt atacgcgcaa ctgccgatcc gcgctcgtgc cttcgttcat gatcgtgtgc
     7321 acgtattcga cggcgctgcg gctgcccagg ccatccacga cgtcatccac caattccagc
     7381 aattcgggaa ccagttggcg catgggcact tcggcttcct tgccgaagtc gatgagtttt
     7441 ccatcaatgc cgtatcgcgc cgcacgccac ttgttctctt cgatgagcgc gcgccggtaa
     7501 agacgccagc tcacattgcc ctggtagagg cggtgcagct tcacaatgat ggcctgcgtg
     7561 agcgccgcga tggccaccgc ttcctcaact cgcgtcgaga cgtcgaacat gcggaattcc
     7621 agggtgccaa acgtggggtg cgggcgcacg tcccaccaaa tcttcttgcc gttgtcgata
     7681 cagttcagct tgacgagaag gttaacgaaa ttttcgtacg cgctccagga ttcaaattgc
     7741 tccggcacac cggtccgagg gaaccggcga aacaccgtcg tgcggaaaga cttcaggccg
     7801 gtatttctgc ccatccaaaa gggcgaactg gtcgagagcg cgagcaggtg cggcaggaag
     7861 tagcgcacgc cgttcatcat gtcgatggtc gtctgcttgt ccggcatggc gatgtgtacg
     7921 tgcattccga agatgagcag cgatcgcgcc agttggccca tctcttcgat gatgtgctga
     7981 tagcgctcgc cgggagagat aacctggtcg atccagcttg aaaaggggtg cgtgcctgcc
     8041 gcggccacct ggagtccggc gcgttcggca gcggcgacca gctcaccgcg tgtgcggtgc
     8101 atttcgatgc gcagctcgct gacgttttcg cagatgcgcg tgccggtttc gacgatggac
     8161 tggtgcagct cgtgcttcac ctgctcagcg atgtccgggg aagccgcgga gacgagctgg
     8221 ctgacg
//
LOCUS       DASYVO010000557         1575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_239514, whole genome shotgun sequence.
ACCESSION   DASYVO010000557 DASYVO010000000
VERSION     DASYVO010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239514"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1562
                     /locus_tag="VGR03_19220"
     CDS             189..1562
                     /locus_tag="VGR03_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996472.1"
                     /translation="MGQKAKWEYFRAIYERYRQADRKRKQAILNEFCVNTGYHRKYAI
                     RLLNGSPPGKAARGAGRTRRRGLSYSREMLSILAAVWEAAGYPWSVRLKALLPLWMPW
                     IRKRFRVRPEIARQLLSISPRQMDRRLRANKTQQKRRIYGRTKPGLLLKHQIPVKTDS
                     WDVTTPGFTEVDLVSHSGNSADGEFAHTLNVTDIHTSWTESRAVLGKGQTAVKEALNE
                     VESALPFRLLGVDSDNGSEFINWHLKAWCESKEIQLTRGRPYKKDDNAHVEQKNWTHV
                     RKLLGWDRYDSLDAVETINDLYRNELRLWLNLYQPSVKLVKKTRVGSKLRRVYDAPQT
                     PLERVASSPQANSACVAKLKDCRKSLDPFRLAKVIDRKLERIYKLTNRRLSPKPMKEN
                     QIMASGRGKGCGKAAPGKSTNRISPPLGNPAQPAGFPLSHSHDGGAPPPSVTFQMARQ
                     PRLRLHS"
BASE COUNT          354 a          437 c          500 g          284 t
ORIGIN      
        1 cttcggcaaa ctcatcgaaa tagccctctt ttcggatgat tcactgcgtt ttcgtgggcg
       61 ctactttact tccattgcga ggcagacgca agaccccgta tcgcgccgtc taaaatgtaa
      121 ccgaagggag tatcgtcgcg ccatttaaga tgtaacccaa ctcggaggcg ttctgaggag
      181 ggttgccaat ggggcaaaag gcgaagtggg agtacttccg ggcgatctac gagcgttacc
      241 gccaggcgga ccggaagcgg aagcaggcga tcttgaatga gttttgtgtg aacaccggct
      301 atcaccggaa gtatgcgata cgtcttttga acggttcgcc tccgggcaag gcagcgcgcg
      361 gcgcgggccg cacccggcga cgcgggctga gctacagccg ggagatgctt tccatcttgg
      421 ccgcggtttg ggaagcggcg ggatatccct ggtcggtgcg gctgaaggcg ctgctgccgc
      481 tgtggatgcc gtggattcgc aagcggtttc gcgtccgccc cgagatcgcg cggcaattgc
      541 tgtcgatcag tccccggcag atggatcgcc ggctgcgcgc gaataagacg cagcagaagc
      601 ggcggatcta tggccggacc aagccgggtc tgctgctcaa gcaccagatt ccggtgaaga
      661 ccgacagctg ggatgtgacg acgccaggat tcacggaagt ggatttagtt tcgcattccg
      721 gcaattcggc ggacggagaa tttgcgcata cgctgaacgt gaccgacatt cacacgagct
      781 ggaccgaatc gcgagcggtc ctggggaaag ggcaaaccgc agtaaaagag gccctgaatg
      841 aggtcgaaag cgcgctgccg ttccgtctgc tgggggtgga ctcggacaac ggttcggaat
      901 tcatcaactg gcatctgaag gcttggtgcg agagcaaaga aatccaactg acccgaggac
      961 gaccctacaa gaaggacgac aacgcgcacg tggagcagaa gaactggacg cacgtgcgga
     1021 agctgctggg ctgggaccgc tacgattcac tcgatgcggt ggagacgatc aacgacctgt
     1081 accggaacga gctgcgcctg tggctgaact tgtaccaacc atcggtgaag ctggtgaaga
     1141 agactcgcgt gggatcgaaa ctgcgacggg tctatgacgc cccgcagacg ccgctggaac
     1201 gcgtggcgtc ctcgccgcag gccaactccg cctgcgtggc taagctaaaa gattgcagga
     1261 agtctctgga tccgtttcgg ctggcaaaag tcatcgatcg aaagctggag cgcatctaca
     1321 agctgacgaa ccggcggctg agcccgaaac cgatgaagga aaatcagatc atggcatcgg
     1381 ggcggggaaa aggctgtggg aaagccgctc cggggaaatc caccaacagg atttcccctc
     1441 cgcttggaaa tcccgcacaa cccgcgggat tcccactttc ccacagccac gacggcggcg
     1501 cgcctccccc ttcggttaca tttcaaatgg cgcgacaacc ccggctacgg ttacattctt
     1561 gaatggcttg acgcg
//
LOCUS       DASYVO010000558        36076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U6256
            k141_196279, whole genome shotgun sequence.
ACCESSION   DASYVO010000558 DASYVO010000000
VERSION     DASYVO010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955709
            Sequence Read Archive: SRR12277200
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 36076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:04:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,871
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 9 of 18
            Pseudo Genes (internal stop)      :: 9 of 18
            Pseudo Genes (multiple problems)  :: 3 of 18
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36076
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196279"
                     /isolate="SMAG_U6256"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="Switzerland"
                     /lat_lon="46.49 N 9.93 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(864..1799)
                     /locus_tag="VGR03_19225"
     CDS             complement(864..1799)
                     /locus_tag="VGR03_19225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996473.1"
                     /translation="MTIQHVSEFPATAPEQIDHFAKLLSKSDQRRKVFWEIYRGKSKR
                     AKTAEEIGKRVGLTPKRVLEIATPLAANNLFEKTKDNGRTAYRKYQNINTVKQKIMGL
                     ASNTAKLEAHITTRKPKVSSSEIRVRVDRRAAEVFADVRFLTIDDVHNFSKVRSLKHK
                     QLPSELDPPKLPEKVFKYGMANILGNKGTFKDWGGEKSDLYTSNLKIKGKRYASAIGF
                     KGPATTGTLTPRKMGHNGDQIQRLFDGDAQVFIVQYEGPMAESIQQQLKGLAVNKSVQ
                     DHRTVFYGTIALEDSYRLRMRYAKEFKKAANKSPS"
     gene            complement(2122..2919)
                     /locus_tag="VGR03_19230"
     CDS             complement(2122..2919)
                     /locus_tag="VGR03_19230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996474.1"
                     /translation="MPKKPESGDLRSLLATKLGVTERQVYNRAKELANRASLKPAEAI
                     WLLAAQSQINLSKYLPPEVVDRVRGLLLQIPAPPKTAAPFATKQIQVATKAAPKEFIV
                     AREFKGSDPILSSQVLNEAREMAAIYPLLYVVENSMREFIRRIVDARLGVSWWTTLSP
                     TAIRRQISTRMTDEKKNAWHQRRGNHPIDYLDLIELPAVVRSNELKFIPDFLPTLRWF
                     EQFVEELYQSRCVVCHMNPLEKDNIQDVKLKATKWQKHVKEKKDTLP"
     gene            complement(2919..3173)
                     /locus_tag="VGR03_19235"
     CDS             complement(2919..3173)
                     /locus_tag="VGR03_19235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996475.1"
                     /translation="MSDEALLKISEQLNQLVALAALNAVKGMKPTEAILTLGSAGLDR
                     NFIAEITGSAPATVSVRLSEAKAKKNPSEKKATKAHRERR"
     gene            complement(3899..5557)
                     /locus_tag="VGR03_19240"
     CDS             complement(3899..5557)
                     /locus_tag="VGR03_19240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1996476.1"
                     /translation="MTRFLGKGISLSVAVGLLIFGAGRAAAHDDRDDGDQNSKNMKLV
                     GFNDLQNRSTYQPTLHKQVVHGHVRYIIYAGHHTLGTPGEGLAPAGTPALPSFNPLTG
                     NNEENGTAIVDVTDVRHPKQLFHLPVSNGTGGGAQMVRVCDGIGANVGKVFMLRSYAS
                     SAHEIWDVTDPSHPAGVRTVAGGNPVIGAQSGAPGALAGTHKSWWECDTGIAYIIGRR
                     GNDTASGWRGGNHIFIFDLSNPATPVFLRDWALDGQQPGGVIPPHFTAVPSIHGPIST
                     GPDTLTNPIAGTGATLDRVYFAYGTGSNGVMQIVNRTELLPPPFGTGVKCGSAASTLT
                     APSCATADFKTAELGRWIMNPDNGAHTNFPLGRLTVQDFVTDTGNDVGTVRDIVFVAS
                     EATANFCANQVRHLTHTVDVTAEGKPQSIATYQVSAASGRFCDRGGRFGPHATNEEFG
                     APFYQKLVFVSEFNAGVRAVDVRDPYSPKEAAFFIPAVTAATDLRCGPIKGNPNKCVQ
                     AIQTNNVATDDRGFIYIVDRADTGMHILRLTGKAAEIAGLEDDD"
     gene            5778..6248
                     /gene="rpiB"
                     /locus_tag="VGR03_19245"
     CDS             5778..6248
                     /gene="rpiB"
                     /locus_tag="VGR03_19245"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEV1996477.1"
                     /translation="MNEASAKLRIAIGADHAGYPVKESIKKYLESAGYAVDDEGTWSE
                     ESVDYPDYGKAVGERVAAKQDDYGIVVCASGIGISIAANKVPGIRAALAHDVTTARLS
                     REHNDANVLAMGGRVVTDALAIEMVKTFLSTPFAHGRHQRRIDKIAEMEKEVGG"
     gene            6327..7634
                     /gene="glyA"
                     /locus_tag="VGR03_19250"
     CDS             6327..7634
                     /gene="glyA"
                     /locus_tag="VGR03_19250"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV1996478.1"
                     /translation="MSRTTEANERMERPLEAVDPEIAGWLGLEAKRQATGLELIPSEN
                     LVSEAVLEAMGSIFTNKYAEGYPGKRYYGGCEFADKVEQLAIDRAKELFGAEHANVQS
                     HSGTQANVSVYMSALQPGDTVLGMNLAHGGHLTHGHPLNFSGRMYKFVAYGVRQDTEQ
                     IDYDELERLANEHKPKMIVAGASAYSRIFDFERLAKISRAAGALLFVDMAHIAGLVAA
                     GVHPSPIPHADFVSTTTHKTLRGPRGGLVFCKAAFAKELDKLTFPGTQGGPLVHTIAA
                     KAVCLKEAMEPGFREYQKQVVANAKAMAAAVARGGFRIVSGGTDNHLFLIELHPRGIT
                     GRDAEQALDRAGITVNKNSIPFDPFPPMKAGGIRLGSPSVTTRGMLEPEMERIGAWIT
                     EILSHLGDAGVEQTIRKQVQEMAVRFPIYQRRLPAAQAAEARR"
     gene            7668..8396
                     /locus_tag="VGR03_19255"
     CDS             7668..8396
                     /locus_tag="VGR03_19255"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HEV1996479.1"
                     /translation="MNNFGTTSRATVLLFTWAAGSVDAIAYLAGHVFTANMTGNAVLL
                     GVSTGELKSTAAARALLALVAFIAGVVLGALLVREGGLAKASADVRRAVIVEAIILAI
                     FAAVAMLPLPLGEGARVNLLIGVSGFGMGLQSAVVRRLNVPGIATTYITGTITSLFSG
                     LVHHWDVLKGGDSGEAEAGPPSEKKRSLALQAEVFISYALSALVTAALHAQWPSGVAL
                     LPFGAIAMVAVFLSVRKSVQVVTT"
     gene            8613..9071
                     /locus_tag="VGR03_19260"
     CDS             8613..9071
                     /locus_tag="VGR03_19260"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1996480.1"
                     /translation="MLDTEAFREFKQGLTLLRSDYPTKALPHMRRAVELEKNNAYYLS
                     YLGVILARAEERWAEAEQLCDSAVRMLRSQAQLYLNLAEVYTQAGRREDAVETLETGL
                     KFARRDVRLNMALRRLTERRPPVLAFLSRQHPVNRQLGILRHQTLRIFNP"
     gene            complement(9287..11065)
                     /gene="glgP"
                     /locus_tag="VGR03_19265"
     CDS             complement(9287..11065)
                     /gene="glgP"
                     /locus_tag="VGR03_19265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEV1996481.1"
                     /translation="MTDPSEFDSRSIAYFTMEIALENAMPTYSGGLGVLAGDTIRAAA
                     DMKVPLVAVSLLYRGGYFSQRLESDGTQLEEPVEWRVEDFLRQEEARTSVTLDGRPVA
                     LRCWRYSVRGVSGYEIPVYLLDADLPVNDPASRALTGTLYGGDAYYRFCQEVILGIGG
                     ISMLRALGYSTISRYHMNEGHASFLAVELLNEEAARAGRRSIRAEDVQSVRRKCLFTT
                     HTPVPAGHDRFPMEMVARAFRHRSDFLDPADAFSSELLNEVVQEDGPFKDFAEAVRAG
                     ANLNMTYLALQLSSYVNGVAKVHGATSRRLFADLRIESITNGVHAATWASPSFQQLFD
                     ARIPGWREDNFSLRAALGLPQEEVWQAHQAAKRRLLEFVHVKSGLSLDPDLFTMGFAR
                     RATAYKRADLILSDLDRLKEIAARAGPFQLVFAGKAHPGDAGGKEIIRSIFRAKKALR
                     RDLRIVYLDDYDMALGGLMTSGVDLWLNTPQFPLEASGTSGMKAAVNGVPSLSVLDGW
                     WVEGHIEGVTGWSVGDDRGVGNPGPDRRGEAESLYRKLQEVILPLYYQQRERFISVMQ
                     HAIALNGSFFHTQRMLQQYVTHGYFR"
     gene            complement(11263..12660)
                     /locus_tag="VGR03_19270"
     CDS             complement(11263..12660)
                     /locus_tag="VGR03_19270"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEV1996482.1"
                     /translation="MTSLIPTEHIIEAVYLVASALFILSLKWMNSPATARRGVLAGEI
                     GMLLAIAGTLLHRGIVDYKWVAIALVLGTVIGVPLGRVQMTAVPQRTALSHAFGALCV
                     TLVGTAEFYLRSPQVPRFMMAVLSLEVILGSLTFTGSLMAAGKLQELLPQRPLTYKGQ
                     NLVNLSLLASSIVVAVYLVLHPGAKPFFPLIVGIPLLFGVLMVIPIGGADMPTVISLL
                     NSYAGLSAAAMGFVLDNKLLIVAGALDGSSGLILSIIMSKAMNRSFTNVLFGAFGKVQ
                     PSVAAGQEAKTVRSATPEEAAGILAAANKVVIVPGYGMAVAQAQHKVRELYDALTRRG
                     VDVKFAIHPVAGRMPGHMNVLLAEADIPYDRLLDMEEINSDFPQTDVALVIGANDVTN
                     PAARTDASSPIFGMPILNVDKARTVMVIKRGMSPGFAGIDNPLYYFDKTLMLFGDAKA
                     FVGNILRELGGATHS"
     gene            complement(12665..12964)
                     /locus_tag="VGR03_19275"
     CDS             complement(12665..12964)
                     /locus_tag="VGR03_19275"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEV1996483.1"
                     /translation="MPSHLIDSLFIFMMAGFIGFEVIRRISPLLHTPLMSLTNALDAI
                     AVVGAIVLAGEHKSTLSVVLGSVAIVAATSNIVGGFVITDRMLRMFKSSRPGKPS"
     gene            complement(13005..14228)
                     /locus_tag="VGR03_19280"
     CDS             complement(13005..14228)
                     /locus_tag="VGR03_19280"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEV1996484.1"
                     /translation="MAGGWPQNEPRHKEIGKVILGVPKEIYPGERRVALVPIVIPNLL
                     KTGFEVLVEAGAGVEAGYPDSHYVEKGAKIVPERSQVFASAGIIVQVLCYGSNDVTGK
                     ADLPLFRRDQVLIGFLRPLGSREIVQQIAATGVTSFAIELMPRTTRAQSMDALSSMGT
                     ICGYKAVLVAAGVLPRLFPMLTTAAGTITPARVLVIGAGVAGLQAIATARRLGAVASA
                     YDLRPAAKEQVLSLGGRFIELPIEAKDAEDSSGYARAQDESFYQRQRELLGRVVAESD
                     VVITAAVIPGKESPVLVTEEMVKSMAPGSVILDLAAERGGNCALSQPGENIVRHGVTI
                     IGRINMASGVPYHASQMYARNITAFLLHLVKDGNLLLNLEDEIVRDTLLTRGGEVVNA
                     RVREFFSLPALVPHG"
     gene            complement(14360..15352)
                     /gene="gmd"
                     /locus_tag="VGR03_19285"
     CDS             complement(14360..15352)
                     /gene="gmd"
                     /locus_tag="VGR03_19285"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEV1996485.1"
                     /translation="MTRALITGITGQDGSYLAEFLLEKGYEVYGLVRRSSLEKYDRLE
                     SVAGRIQLVEGDLTDQGSLDVALQSVQPDEVYNLAAQSYVAVSWNQPSFTADVTGLGV
                     LRVLEAIRRHAPKAKFLQASSSEMFGKVGGPSQNEDTPFHPRSPYAVAKVFGHHITVN
                     YRESYGFFASSCIAFNHESPRRGTEFVTRKITQQVARIKLGLADRLKMGNLDVQRDWG
                     FAGDYVRAMWMILQQPKPDDFVIATGRPHSVRELLETAFSHAGLNWKDYVDVDPSLFR
                     PAEVEYLCGDPSKARRALGWEPRVAFRELIEMMVRSDLASLKQTAAARALNSDP"
     gene            complement(15626..22906)
                     /locus_tag="VGR03_19290"
     CDS             complement(15626..22906)
                     /locus_tag="VGR03_19290"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEV1996486.1"
                     /translation="MGRLRLPAGFRAVSFFCAGLLACLVFVTGLASQQNTPSGPRIWL
                     QEPRSLPVKYVAAAVGASSGATVAAQNVNDGAAQILASGQGQPLAMIAGDFDRDGVND
                     LVVGYAVPGGGILAFHRGNLDAFAPQSDASFQAIGRGEFPSPFLPGVQVTRLAIRPDF
                     LAVGNFTGQGDLDLVVAARGGNAIYLFAGDGTGKFANPQIITLTGGITALAAGEFGNG
                     HSLTNLAVGISGPNNSFSFVILGETGVGLGALASYPLSAPASNILFGDFGGGPDAAFI
                     SGGHIQILRSSNMQLATVSLPVSASAFALGSFIYDRNSGSQIALLASDGSIQIAVRYE
                     FDPRVYTVEEFSAIRQAKLNHQPPPSFVPAPSFPVNGWRIVEGFPSVANISSSQAPVF
                     FRTRVSSNGADDIMWLSAATGQMAVISHPDVPPGASTFLPGQLSLKPYSGSPVAALPM
                     RINVDGRPGIMALHQGEIAPFMVMPIPDPTFTVNTTLDVLTPGACAAALPGCSLREAI
                     IEANATPGTDTIMVPAGTFSLTRGRAATPLYDAQTGTLNINDSVNIIGAGQNTTIITW
                     GTPVGGPVDMVMAVNEDIPSATVPTTTTATASISSLTIQNGVNHGTHNNDGDGGCMEF
                     DTGTSGTATLSLTNVTIQNCATTQGGGGGLVTFAFVNPAGGGTVTISNSVFQNNSAVD
                     SVAGTEGGGIAISAGSLVTITNTLVQNNTSTNVVSGQAGLGGGIALDACCATPPFWGS
                     TTIHSSTISGNQAAGFGGGVSDIAMGLIVDQGTVISGNKGGLGNVVNAKDGGGVYLSL
                     GAFGCPGSCSFPNTVTFNKVAIINNSTTGNGGGIHTGSGGTFPTAGPMSITFSRLAGN
                     TAGGSGSNLSNSGTTVTATNNWWGTNAAATTINTTIAGATTTFDPFIVLTHDATPAII
                     KINGTTTLTGDMSQDNHGNGAALSGNLDRIVGLPITFDNPVLGTIPQAQPETLNASAQ
                     ATATFNAGGASGRGSAHATVDQAVVSANSNLIASASEAVNTVTITTVGAHNFAAGETV
                     VISGVGVGGYDGTFAITATPTATTFTYTDPTPGLGASSGGLANVGIIILEPLQITKSF
                     NPATVAVNAPSALTFSVVNPNVIAVDASFTDTFPANLVVANPPSVTNTCGGTFTPAAG
                     DTSVTFTNASLAVGSCTITVHVQSATDSVYPNSVTINSTAAGTGAQSTSSASLTVINP
                     PTIAKVFGAATIPLNGTTSLIFTFSNTNSNTTLNGIAASDTFPAGLVVATPGNVSTTC
                     NGTGSAPDGASTVSLSGASLAPGASCTVTMNVTGTTAGLKSNTTGNVTATDAGGITGS
                     TASASITVVGPPTISKAFNPTSIALNATSTLSFTITNPNTASALSGVGFTDTLPSGVV
                     VAATPNITGSCGSGTITAVAGSGTISLTGGTLTASPAAGSSCTFSADVTGTTAGSKSN
                     TTGAVTSTEGGTGAPSNTATVTVVAPPTISKAFGAAAIPVNGTTTLNFTITNPAANTV
                     AEAGVAFTDTLPAGLVVATPNGLTNTCGGTATAVAGSGSISLTGGAVAASSTCTVTVN
                     ITGTASANYTNTTGAVSSTNGGTGNTASANLAVATPPTIVKSFNPITIFLSQNSTLSF
                     TIQNPNTSVSLTGVAFTDNLPGGLVVASPNGLTGACGGGTITATAGAGTISLSGATLA
                     ANASCTFSVNVTGTAASVKNNAVQVTSTEGGAGNTSNASITVNAATPPTISKSFAQSA
                     IGQGSTVLLSITIVNPNSNPNPNVPLTGIAFSDTLPAGLTVNFNTLNTDCGGTLTATP
                     GSISLTGGAIDPGAPPPPSRGRRGDAVTRRVGPKGNAVNAAGFCTVNVNLLVSGSGTL
                     SNTTGAVSANESGTGAVSNTATIDVIQAPTVAKAFGAASIPLNGSTKLTFTVGNPNAS
                     TSLVGIAFNDTLPSGLVVASPNGLPNGCADPNANVTGDVVANPGSNTISMTALSLNQS
                     VSCSFFINVTGTTGGTKNNTTGNVTATFDDGSGTFLPVTGGTASASVVVVAPPSIAKS
                     FNPTAIAVNGTSTLTITITNPAANTVAEAGLAFTDTLPASMVVATPNGLTNTCGGTPT
                     ATAGFGSVSLTGGTVAAASICKLTVNVTASSTGNFTNTTGAVSSTNGNTGNTATANLT
                     VQPADLTITKTHTGNFSRGQTGATYTITVTNSGQGPTVGTVTVVDNLPNVPNTLVATA
                     MSGTGWTCTLGTLTCTRSDVLAPAGSYPPITLTVNVPLNIQANVTNTATVSGGGETNT
                     GNDTASDQTHIGPPIQITFNVSTLTVSRGNSTSTTFTVDSSAGEGTINFSCSGLPPGA
                     SCSFNPPSESQLTATVTLTIATSAGSASAPPFGGEGTTPVYAVLLPLLGLVGIGLGTV
                     KGKKARLRLALCLGGVVFVLATFAGCAGGPRGTPTGAFQITVTATSASTGDSGTATIT
                     LNVL"
     gene            complement(23144..23908)
                     /locus_tag="VGR03_19295"
     CDS             complement(23144..23908)
                     /locus_tag="VGR03_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1996487.1"
                     /translation="MMASSSSNPAGAASRPPGKPLLELKDATAFYGDLQALFGITLRV
                     QAGEIVTLIGANGAGKTTLLRLISGIKQLAGGSLRFDGEDVSHVPAHEMVARGLSHVP
                     EGRQVFPFLTVRENLSLGAYTPRARTHVKRLMAEQLALFPRLADRLDQLAGTLSGGEQ
                     QMVAIARGLMAQPKLLLLDEPSLGLAPKIVEEMFEKIQEVRRTGITMLIVEQNVVSGL
                     GISDRGYVVQNGTVLLEDTSANLLTNEMVRAAYLGL"
     gene            complement(23905..24642)
                     /locus_tag="VGR03_19300"
     CDS             complement(23905..24642)
                     /locus_tag="VGR03_19300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1996488.1"
                     /translation="MTPILELQNVCKRFGGLAAVKNVSFTLNEGEILFIIGPNGAGKT
                     TVFNLISGFLHPEEGAILFQGKDISRLAPHRAARLGIGRTFQIVKPLPNLTVLQNVML
                     GAFMHSGNVHKAEEEAGRILAFLQMSHLGHSPARGLPLAALKRLEIARALATKPRLIL
                     LDEVVSGLTTSEALALANLVKRLPEWGVSVVGGVEHVMQVVMKISDRIVVLDYGAKIA
                     EGKPSEVVNLPVVIEAYLGPKYKELAR"
     gene            complement(24639..25646)
                     /locus_tag="VGR03_19305"
     CDS             complement(24639..25646)
                     /locus_tag="VGR03_19305"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1996489.1"
                     /translation="MSSLKSWKLWTLLGAVIACAATPLFVQNDYQMQVLFRIALFAAL
                     GLAWNVVGGYAGQLSLGHVAFFGLGAYGLALFTALSIPSWISMFLAAIVATLFAAVIG
                     RIVFRLRGPYFTLATIASAEVLRLVATNLNVTGGAIGLTTPALFTGKTFWDLFYLSAV
                     ALALASFLVNYWVSRSRFGYYLMAIREDEETASAVGIDTAGYKLRSLLISAFLTALAG
                     ALYGSAFQFIVPDSILSLEISIQIAIITMLGGAGTLLGPIVGAVLLLSASEVFKNQFK
                     ESHLLIYGILIVVVVLFLPEGIVGGLQHRLRKRIKPPAAPEAAGPPNSKAPAALEETS
                     R"
     gene            complement(25643..26509)
                     /locus_tag="VGR03_19310"
     CDS             complement(25643..26509)
                     /locus_tag="VGR03_19310"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1996490.1"
                     /translation="MIAFLQACMNGLLTGALYALIGMGLALIFGVMRIVNFAHGAFLM
                     VGMYATWVLFDKLGINPYLGFAPAGLFLFLFGYLVYQLVIKPVRNQSHFMQILMTTGI
                     ALILTDSAQLIFHADYRQTNIALINRTLRFGPFTANAAALLSFAIAAVLIAGLTLFVT
                     RSLTGQALRAIAQNPEVAPLMGINVSRIHGFSFALGIALAGMAGGLLLPALYLFPSVG
                     EDYTTKAFVIVVLGGMGSIEGAALGGLVLGLAESLTSLYLGNQWALVVDFTIFLLVLS
                     FKPSGIFGRQRV"
     gene            complement(26591..27790)
                     /locus_tag="VGR03_19315"
     CDS             complement(26591..27790)
                     /locus_tag="VGR03_19315"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1996491.1"
                     /translation="MRSKTVAASLVVFIGISLAVSCQKKGGEGDTIKIGVITSLTGSL
                     AAFGEAHKHGYSIALDELNAKGGALGKKIELDFYDDQSKPDQAVQGVSKLVDQDHVPI
                     LLGSYSSETTKAIIPSVTQRQVPLIMPTATADNVMESKSPWVFRVCAGATDYARTTIA
                     FLKDSGAPKTMAIVYENTNFGQSNMKAMTAAAQRAGINLVAVESYEAKSPDYKAVLQR
                     VKKANPEVIYFCSYLLDAGTLLRQAQELDLNPRYYTSAGTGFAAAEFPTPKGAGKNAE
                     YTFSVSQWLPEAQWAGSKEFDAEYAKRYNSHPAYHAIEAYAALYVAVQAINDAKSSDS
                     AKIRDAVKNLNLTSTPFGPIRFDSNGQNQHPVLITQVQGGRYRVVYPANAADSKPIIP
                     APRWSQR"
     gene            28206..29309
                     /gene="had"
                     /locus_tag="VGR03_19320"
     CDS             28206..29309
                     /gene="had"
                     /locus_tag="VGR03_19320"
                     /EC_number="1.1.1.368"
                     /inference="COORDINATES: protein motif:HMM:TIGR03201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxycyclohex-1-ene-1-carbonyl-CoA
                     dehydrogenase"
                     /protein_id="HEV1996492.1"
                     /translation="MASVLGAYGYQLTAPGTPLQRYEFPALDPGPDEVIVQVAGCGVC
                     HTDVGFALDGVPTRHSLPLVLGHEISGRVVAAGEIAGGWLGRSVIVPAVIPCGACAAC
                     LAERPTICRKQFMPGNDGNGGFASHIVVPARGLCAVPENLPSGLPLEMLSVVADAVTT
                     PYEAIRRAGLGPEDVAVVVGVGGIGGFGVQIAAALGAAVVAIDIDQERLSQAARHGAG
                     LVLNARITDLKGLKGAIRAFAKEAGRKGIGLKIFEMSGTPAGQTTAFGLLDHGAYLAI
                     VGFTAKPVELRLSNLMAFDATARGNWGCPPDQYPKALRLVLEGKISLAPFTEQHPLEE
                     APAILEAVARHELRRRVILRPNHGKGKRESEGA"
     gene            29306..30412
                     /gene="oah"
                     /locus_tag="VGR03_19325"
     CDS             29306..30412
                     /gene="oah"
                     /locus_tag="VGR03_19325"
                     /EC_number="3.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018807 -
                     6-hydroxycyclohex-1-ene-1-carboxyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase"
                     /protein_id="HEV1996493.1"
                     /translation="MMKDHNLVEAAPPRNILVERKPARDRSGQRVESLHNVWITLNNP
                     KELNSYTTDMVKEVILALREASNDRAAVVVVLTGAGTSSFCSGGNTREYAENYAGAPG
                     EYRQYMRLFNDMVTSVLQCDKPVICRVNGMRVGGGQEVGMACDFSIASDLAFFSQAGP
                     KHGSVPDGGSTDFLPVFVGIERAMHSGTLCEMWSAHRFYWLGGLTQIVPALRVGDKWV
                     ANPLVVTDRVLDEYGRLALGEWKMGQAREEGKALMAKGTVDLSLLDEAVEQTCTKLLY
                     TMPDCLIKTIESLRKHKLEHWDKNRETNRAWLALNMMTEANAGFRAFHYGTKEEREVD
                     FIRLRQRLAEGARWGEELIREVAPWVAACAKTSK"
     gene            30464..31636
                     /gene="bcrC"
                     /locus_tag="VGR03_19330"
     CDS             30464..31636
                     /gene="bcrC"
                     /locus_tag="VGR03_19330"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit C"
                     /protein_id="HEV1996494.1"
                     /translation="MKPELTELVRRARELSDDLDLKTVSAWKEAVPGRKAVGHMPIYV
                     PRELIHAAKMLPVGILGGGDRLEIIRGDAFFQSYICHIPRSTIELALTHRLDALDGML
                     FPSICDVIRNLSGMWTMLFPDRYVRYVDVPQNYEPGVGGLYWRQELEILREDFRRLSG
                     NPVSDDDLRHSIAAYNENRKAIRDFYQARGRQPWFYPISEAYLFLRAGNLLPVEEHTA
                     LLHEYLRLAPLEDDRVEQDESRVVLVGMFCEQPPLGLLATLERAGCRVVDDDLLLGLR
                     WITSGVSTGGDPVQNLAEAFLNQSATTASRYLPDGKHGLSLIDSVRRNHAEGVIFCAP
                     SFCDPALLEQPMLVAALDREKIPHTQFKYSEDTGQFAVIREQAGTFSDSIRLWSEA"
     gene            31633..32949
                     /gene="bcrB"
                     /locus_tag="VGR03_19335"
     CDS             31633..32949
                     /gene="bcrB"
                     /locus_tag="VGR03_19335"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit B"
                     /protein_id="HEV1996495.1"
                     /translation="MSSAPQKKLPAQDVAQKERSMLLQKQMIASHYDALAAAHDSGRK
                     VVYTFVPGNLTELMRSFDVLPVLPEINALQSGMRKLSGEYISEAEKYGHSEDVCTYVK
                     CDIGMLKKGNIGPTGKKLPPPDLLLLSYTGCFTFMKWFELLRQEYDCPVAMLHVPYQS
                     DGKIHSHHLDYILQQLRTEVIPKLEQVTGLRYDEDRLREKMKLSARAEDDFVWVLESA
                     KNIPSPIDAYFGGVYYIGPMFTAFRGTPEGVEYYRTLREEIEERVAQHLGPITPDGPL
                     EKERFRLVMEGPPNWTSFREFWKMFYEEGAVIVASSYTKVGGLYDRGFRHDPERPLES
                     LAEYCLGCYTNLGLPTRLGLLERYIRDYHADGFMINSVKSCNSFSVGQLLMLRQLEER
                     TGVPGGFIESDLVDPRYFSAANIKNRIESYLQMLDQKRGARAGVQP"
     gene            32955..34277
                     /gene="bcrA"
                     /locus_tag="VGR03_19340"
                     /pseudo
     CDS             32955..34277
                     /gene="bcrA"
                     /locus_tag="VGR03_19340"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit A"
     gene            34277..35152
                     /gene="bcrD"
                     /locus_tag="VGR03_19345"
     CDS             34277..35152
                     /gene="bcrD"
                     /locus_tag="VGR03_19345"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit D"
                     /protein_id="HEV1996496.1"
                     /translation="MATTAQLPPRAEEALVTAGVDVGSSSVKIAILQMNAGDSVRVLG
                     LRIDRIRRRDLRKVIHEGFEVALEEAGVKRSDVDYVASTGEGELVDFRRGHFYGMTTH
                     ARGALFLLPETRAVLDVGALHARAMTIDERSRVLNYRMTSQCASGSGQFLENVARYLG
                     IALEDVGPLSCRATNPQSVSSICAVLAETDVINMVSRGISTADILKGIHLSMAGRYLK
                     LLRSIDATGHVAISGGLAADTGLARALEEKIKEEGLSLQVHTHPQSIFAGALGAALWG
                     GYRHRVLARRAAVRA"
     gene            35174..35674
                     /locus_tag="VGR03_19350"
     CDS             35174..35674
                     /locus_tag="VGR03_19350"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1996497.1"
                     /translation="MEQAPMEMPGTLETDAVVVRTMRENDLEAVVSIDAAAAGRRRPR
                     YFELMLKRALTQTGVQISLVAEIGGRVVGFLIGSLYYGEYGVMEPSASIDAIAVHPDF
                     RGQHVGKALMRQMRLNLGALRIAVLRTEVEWDDFTLATFFRSEGFTPARRLCLECSLD
                     PTSPRD"
     gene            complement(35994..36068)
                     /locus_tag="VGR03_19355"
     tRNA            complement(35994..36068)
                     /locus_tag="VGR03_19355"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36034..36036),aa:Lys,seq:ctt)
BASE COUNT         7231 a        10101 c        11688 g         7056 t
ORIGIN      
        1 ctcagaagcg tagcggaggc gtgggagtgt gtcaacgaaa ggggcggggg ggccgtggac
       61 cgtgtcccgt gatccgtggt cgggggagat acgcgtctag ccggatggct aacggacatg
      121 tttaccaccc cccaccccct attgttttcg caagagtgcg gaagcgaaag aaagaaaagg
      181 ggttgcgacg attcctgcgc acggcaagag tgagaagagt gcggaagagc ctgaaaagaa
      241 aggagatgaa tcgtgattgg tggctgggga ttggtgactc gtgactggga agcgttgact
      301 gaaacgatga ccgggagatt tccacagggc agaagccccc tcccccccct gtttcttgga
      361 agtgcggatt ctaaaagggt tacgggccca ctttctcgta ggtgcggaat ccaaaggggt
      421 tataagtctt gtgttttccg cagttacgag ccgtgctcgt aagtgttgaa aagagaaggg
      481 ttgcgggaga aattctcgcg ctgtaagtcc tttgttttcc gcagatccga ggggggtctg
      541 caagtgttga ttctgaaggg gttgcgagag cagcttctcg gaagtgcgga atctaaagga
      601 gttgcgggaa gggcggggga gggtgtcggc ggggctggag tcggaagtca cggccaatgg
      661 tagcagggat tttataccat gtcaatagat tacttcctgg tattcgaacg gattgtggaa
      721 aagaattcga gagttcccgc gctgcgaagt cttgaaaaac caaggctttg agaagaaaag
      781 gccgcttgtc ggactcaccc gaagttccaa cgcaccccgc gctctccttg ctgatagtcg
      841 gcgtaacgtg gtgctcgtca ctattagcta ggagacttat tcgcggcctt tttgaactcc
      901 tttgcgtatc tcatccggag tctgtacgaa tcttccaatg caatggttcc gtagaacacg
      961 gttctatgat cttgaacaga tttgttcacc gccaatccct tcaattgctg ttggatgctc
     1021 tcagccatcg gcccttcgta ctgcacaatg aatacttgag cgtcaccgtc aaacaatctc
     1081 tgaatttgat cgccgttgtg ccccatcttg cggggcgtca atgtgcccgt tgtcgctggc
     1141 cctttgaatc cgatagcgct ggcgtatcgc tttcccttta tcttgaggtt gcttgtgtac
     1201 aggtcactct tttccccgcc ccagtccttg aatgttccct tattacccaa aatgtttgcc
     1261 atcccatact tgaaaacctt ctccggtagc ttcggcgggt cgagttcgct tggcaactgt
     1321 ttgtgtttca acgaccgcac tttggagaag ttatggacat cgtctatcgt aaggaatctg
     1381 acgtctgcaa aaacctctgc cgcccttcgg tcaactcgca cccggatttc actcgaagac
     1441 actttcggtt tgcgggtagt aatgtgtgct tcaagctttg cggtattgga tgctagcccc
     1501 attatctttt gcttgaccgt gttaatgttc tggtactttc ggtaggcagt acggccattg
     1561 tcttttgttt tttcaaacag attattggcc gcgagcgggg tggcgatttc caacactcgt
     1621 tttggagtaa ggccgactct cttgccgatc tcttccgccg tcttagcgcg ctttgacttc
     1681 cctctgtata tttcccagaa gactttcctt cgttggtcac tcttgcttag cagcttagcg
     1741 aaatggtcaa tctgttctgg tgcggtagct gggaattcgg agacgtgttg tatcgtcatg
     1801 ggaacctggg aattcccgaa tcgtcagcct tcggttattt cggcttggga acgcctttca
     1861 gaaaatcatt aacccacgtg gccgatttgc caacggcacg ggcaattgta ttttgacttg
     1921 tgcccgcgtt ccacaaatta atcgccacca actttgagag cagctccgca acttggtcac
     1981 tcgggctctg ctttttgcgc ggcatatgtc ctcacttttc cctcttacct gcatatttcc
     2041 ttgggtgaac ggggactcgc gagtggaaag taatcattcg tagagatcat agcaagcctt
     2101 gactcgccgt aaaatatgca attatggcag cgtgtccttc ttctccttga cgtgtttctg
     2161 ccactttgtc gctttgagtt ttacgtcctg aatgttgtcc ttttcaagtg gattcatgtg
     2221 acaaaccacg cagcgtgatt gatagagttc ctcaacaaac tgctcaaacc aacgcagcgt
     2281 gggcaagaaa tctggaataa acttcaattc gttgctcctt accactgctg gcagttctat
     2341 caggtccaaa tagtcgattg ggtgattccc tctgcgttgg tgccaggcgt ttttcttttc
     2401 atcagtcata cgagttgaaa tctgacgtcg gattgccgtt ggcgagagtg ttgtccacca
     2461 cgaaacccca aggcgtgcgt caactattcg ccgtatgaac tcgcgcattg aattttccac
     2521 aacgtagagc agggggtaga tggctgccat ctcacgcgct tcgttcaaaa cttggctact
     2581 gaggatgggg tcactgcctt tgaattcccg cgctacaata aattctttcg gtgcggcttt
     2641 agttgccact tgaatctgtt tcgttgcaaa aggtgcggca gtcttaggcg gagctgggat
     2701 ttgaagtaaa aggccgcgga ccctatcaac cacttcgggg ggcaagtatt tgctcagatt
     2761 tatttgactt tgtgctgcaa ggagccagat agcttctgca ggcttcaggg acgcccggtt
     2821 cgctagttct tttgcccgat tatagacttg gcgttcagtg acgcccagct ttgttgcaag
     2881 aagactccga aggtcaccgc tttcaggttt tttcggcact atcttcgctc gcggtgtgcc
     2941 ttagtggcct tcttttctga cggattcttc tttgctttcg cctctgataa tcgaacgctc
     3001 actgtggctg gcgcacttcc agtaatctca gcaataaagt tgcgatctaa ccccgctgac
     3061 ccaagcgtaa ggatggcttc agtgggcttc attcctttga cagcatttag cgcggctaaa
     3121 gcgacaagtt ggttaagctg ttcactgatt ttcagcaagg cttcgtcact cacgtagcgt
     3181 ttcctccgtt ttctctaatg tgctctgact ttctcagttt tcacaggggg aagatagaga
     3241 agccgatggt agcggggctt tgcatcattg cctatgttgt agactattcc ctgttcttcc
     3301 catgactgta gaaccacact catcgtgggc tgcgacacac cagcaagctt cgcgagctca
     3361 gtgactccgg ccgtaccgtc aagagcgttg tacaccttca aataggcttg gggattccgt
     3421 ttccccccgc acacaatctt tgaaattgtg ggcacgccaa tagtacttcg agcaagcaag
     3481 gcgattatgg caacgttttg acgctctaat ttttctgaca tcccagatca ccccattctg
     3541 tagaatcaaa cgctcaccga aggcactgac ccgccggacg attactctcc aggtagttct
     3601 ccggtcaaga aaccgatgat aagagtacca ataagtgctg aacataacta gcttcgagca
     3661 gttctttcgg ctccacctgg acccggcgat ggaacgctgg cgagctgcga atgtcatcta
     3721 gtcttagttg agttctatgt tccggatatt gggaatcgtg gttttcttcc caacctgctt
     3781 gtcctgtatt tgggtagaga tgagcaggct cagggcagca tacgcggcgc gaaggaaagc
     3841 cgggcgcaga aaaaaagggc gccggatgtg ttccggcgcc cgatttggca catcggtcct
     3901 agtcgtcgtc ttcgagcccg gcgatttcgg cggccttgcc ggtcagccgc aggatgtgca
     3961 tccccgtgtc ggcgcggtcg acgatgtaga tgaacccgcg gtcgtcggtc gcgacgttgt
     4021 tcgtctgaat ggcctggacg cacttgttgg ggttcccctt gatcgggccg cagcggaggt
     4081 cggtggcggc ggtcacggca ggaatgaaga aggcggcctc cttcgggctg tagggatcgc
     4141 gcacgtcgac agcgcgcacg cccgcattga actccgagac gaagacgagc ttctggtaga
     4201 acggcgcccc gaactcttcg ttggtcgcat gggggccgaa gcgtccgccc cgatcgcaga
     4261 agcgcccgct ggcggccgac acctggtagg tggcgatcga ctgcggcttg ccctctgctg
     4321 tgacgtccac cgtgtgcgtc aggtggcgga cctggttggc gcagaagttc gccgtcgcct
     4381 cggaggcgac gaacacgatg tcgcgaacgg ttcccacgtc gttgccggta tcggtcacga
     4441 agtcctgcac cgtcaagcgg ccgagcggga agttggtgtg cgcgccgttg tccggattca
     4501 tgatccagcg cccgagctcc gcagtcttga agtcagcagt agcacaggaa ggagcggtaa
     4561 gtgtggacgc ggccgagccg cacttgacac cggtgccgaa gggcgggggc aggagctcgg
     4621 tgcggttgac gatctgcatg acgccgttgg agccggtacc gtacgcgaag tagacgcggt
     4681 cgagggtagc gcccgtccca gcgatagggt tcgtgagggt gtcgggcccg gtggagatgg
     4741 ggccgtggat gctgggcacc gcagtgaagt ggggtggaat cacgcctccc ggctgctgcc
     4801 cgtcgagggc ccagtcgcgg aggaacactg gagttgccgg gttgctgaga tcgaagatga
     4861 agatgtggtt gccgcccctc catccggacg cggtgtcatt gccgcgccgg ccgatgatgt
     4921 aggcgatacc ggtgtcgcac tcccaccagc tcttgtgcgt cccggcgagg gcgcctgggg
     4981 cgccggactg ggctccgatg accgggttcc cacccgcgac agttcggaca cccgcgggat
     5041 ggctcgggtc cgtcacgtcc cagatctcgt gcgccgagct cgcgtaggac cgcagcatga
     5101 acaccttgcc gacgttcgca ccgattccat cgcaaacgcg gaccatctgg gccccgccgc
     5161 cggtcccgtt cgaaacggga agatggaaca gctgtttcgg gtgccgcacg tcggtgacgt
     5221 cgacgatcgc ggtgccgttc tcctcgttgt tgccggtgag aggattgaat gacggcaggg
     5281 ctggcgttcc ggccggggca agaccctcgc ctggcgtccc cagtgtatgg tgaccggcgt
     5341 agatgatgta cctgacgtgg ccgtggacga cctgcttgtg cagcgtcggc tggtaggtcg
     5401 agcggttttg caggtcgttg aagcccacca gtttcatgtt cttgctgttc tggtcgccgt
     5461 cgtcgcggtc gtcgtgggca gcggcgcgcc ctgcgccgaa gatgagcaga ccaaccgcta
     5521 ctgacagtga aatacccttc ccgagaaatc gcgtcatggt gtcctcctgt tcctaaacgg
     5581 atcgtaaacc ggaatttccg atttcaacct tatctttgcg cagaaccata cggcagccgc
     5641 ccctcgcctg tcaatctcaa atggccatgg acgcggttgc cacccaacag gggatgagcg
     5701 gctgttcggg aggacgaggt tccccgcacg attggaattc caggctccag tccattagaa
     5761 tcctcccgtg aacaggcatg aatgaagcca gcgcaaagct gcggatcgcc atcggagcgg
     5821 accacgcggg atatcccgtc aaagagagca tcaagaagta tttggagagc gcgggctacg
     5881 cggtggacga tgaggggacg tggtcggaag agtcggtgga ctacccggac tatgggaagg
     5941 cggtggggga acgagtggcg gcgaagcagg acgattacgg gatcgtggtg tgcgcgagtg
     6001 ggatcgggat ttcgattgcg gcgaacaagg tgcccggcat ccgggcggcg ctggcgcacg
     6061 acgtgacgac ggcgcggctt tcgcgcgagc acaacgacgc gaacgtgctg gcgatgggcg
     6121 gacgcgtggt gaccgatgcg ctggcgatcg agatggtgaa gacgtttttg agcacgccgt
     6181 tcgcgcacgg gcggcaccag cggcggattg acaagattgc ggagatggaa aaagaggttg
     6241 gaggttagga gtcagaggtt ggaaggaagg ccgaagatgg gccggatgaa tcggcggatg
     6301 gccggagcgg cgcaaggagc ctgggaatga gcagaacgac ggaagcgaac gaacggatgg
     6361 agcggccgct agaagcggtg gacccggaga tcgccgggtg gctggggctg gaggcaaagc
     6421 ggcaggcgac ggggctggag ctgatcccgt cggagaacct cgtctcggaa gcggtgctgg
     6481 aggcgatggg ctcaatcttc acgaacaagt acgccgaggg atacccgggg aagcgttatt
     6541 acggcggatg cgagttcgcg gacaaggtcg agcaactggc cattgaccgg gcgaaggagc
     6601 ttttcggggc ggagcacgcg aacgtgcagt cgcattcggg gacgcaggca aacgtctcgg
     6661 tgtacatgtc ggcgctgcag ccgggcgaca cggtgctggg gatgaacctc gcgcacgggg
     6721 gccacctgac gcacgggcac ccgctgaatt tttcggggcg gatgtacaag ttcgtggcct
     6781 acggggtgcg gcaggacacg gagcagattg attacgacga gttggaacgg ctggcgaacg
     6841 agcacaagcc gaagatgatt gtggcgggag cgagcgcgta ttcgcggata tttgatttcg
     6901 agcggctggc caaaatttcg cgggccgccg gggcgctgct gttcgtggac atggcgcaca
     6961 tcgccgggct ggtggcggcg ggcgtgcacc cgagcccgat tccgcacgcg gattttgtct
     7021 cgacgacgac gcacaagacg ctgcgcgggc cgcgcggggg gctggtgttc tgcaaggcgg
     7081 cgttcgccaa ggagctggac aagctgacgt ttccggggac gcagggtggg ccgctggtgc
     7141 acacgattgc ggcgaaggcg gtgtgcctga aagaggcgat ggagccgggg ttccgcgagt
     7201 accagaaaca ggtggttgcg aatgcgaagg ccatggccgc agcggtggca cgagggggat
     7261 tccgcatcgt cagcggcggg acggacaatc atctgttcct gatcgagctg catccgcggg
     7321 ggatcacggg gcgggatgcg gagcaggcgc tggaccgcgc ggggattacc gtgaacaaga
     7381 attcgattcc gttcgatccg tttccgccta tgaaggcggg cggaatccgg ctggggagcc
     7441 cgtcggtgac gacgcgtggc atgctcgaac cggagatgga gagaatcgga gcgtggatta
     7501 cggaaatcct ctcgcatttg ggcgatgccg gcgtcgagca gacgattcgc aaacaagtgc
     7561 aagagatggc tgtgcgtttc ccgatctacc agcgccggct gcccgcggcg caggctgcgg
     7621 aagcgcgccg ctagttgcgg aaggcgccgt tcctgcggat cgcagcgatg aacaatttcg
     7681 gaacgacgag ccgagcgacg gtgctcttgt tcacctgggc cgccggcagt gttgacgcca
     7741 tcgcttacct ggccgggcac gtttttaccg ccaacatgac gggcaacgcg gtgcttctgg
     7801 gcgtctccac cggggagctc aagagcacag ccgccgcacg cgccctcctg gcgctcgtgg
     7861 cgtttatcgc cggggtcgtc ctgggtgcgc tgctggtcag agaaggcggg ctggccaaag
     7921 ccagcgcgga cgttcgccgg gcggtgatcg tggaagcgat tatcctcgcg attttcgccg
     7981 ctgtcgccat gctgccgttg ccgcttggag aaggcgcgcg cgtgaacctg ctgatcggcg
     8041 tatcgggttt cgggatgggg ctgcagagcg ccgtggtcag gcgattgaac gtgcccggaa
     8101 tcgccaccac atatatcacc ggcaccataa ccagcctgtt ctccggcctg gtccatcact
     8161 gggacgtact gaagggaggc gattccggcg aggcagaagc gggcccgccc tcggagaaga
     8221 agaggtccct cgcgctgcag gccgaagtgt tcatttccta cgcgctgtcg gcgctggtca
     8281 cggcagcgct gcacgcgcag tggccttcgg gagtggcctt gctcccgttt ggcgcgattg
     8341 cgatggttgc ggtcttcctg tccgtccgca aatccgtgca ggtagtcacg acgtagagcg
     8401 ttttcttgaa cggcttggag cgcggcgact gacgcgccgc agcgatggcc tcttcgattt
     8461 gccttcactg gccttgtctt cgcacctgac ccttgcgtgc ggctgtccca agactggccc
     8521 tcaggtcagt atatttcgat tgaattgagg gctaggttgt ctaaagaagt tgtgggctgc
     8581 cgcaattccc ggggattccg cgaggtggaa gaatgctgga caccgaagcg tttcgtgagt
     8641 tcaagcaagg tttgacgcta ctccgaagcg actatccgac aaaagcactg ccacacatgc
     8701 gacgagcggt ggaactggaa aagaacaacg cgtactacct gtcatacctg ggtgtaatcc
     8761 tggcgcgggc agaagagaga tgggccgaag cggagcagtt gtgcgattcc gccgtgcgga
     8821 tgctgaggag ccaagcgcaa ctgtacctga atcttgcgga agtgtacacg caggcgggcc
     8881 gccgcgaaga tgcggtggaa accctagaga cgggactgaa gtttgcgagg cgcgacgtgc
     8941 ggctgaatat ggcgctgcgc cggctgacgg aacggaggcc gccggtgctg gcgttcctga
     9001 gccggcagca ccccgtcaac cgacaactcg gcattttgag gcaccagacg ttgcggattt
     9061 tcaacccata ggccgaaagc catcgatctc ctccaacccc ccaaacgcag gaagggcgga
     9121 cggccaaaag ccgcccgccc ttcttgtttc caaggagaca ggaaagcaaa cgggcgcgag
     9181 gttatccggg caggttcgtg gccggctcgt tgaacctgtg cgcgacgccg acttccatga
     9241 ggagcctaaa tcccaagcgg gaacaaaacg tacgtacatc cagtcttcag cggaaatagc
     9301 cgtgggtaac gtactgctgc agcatgcgct gggtgtggaa gaaagagccg ttgagggcaa
     9361 tggcatgctg catgacggaa atgaagcgct cgcgctgttg gtagtagaga ggaaggatca
     9421 cctcctgcag cttgcgatag agggattccg cttcgccgcg ccggtccggg ccgggattgc
     9481 cgacgccgcg atcgtcccca acggaccagc cggtgacgcc ttcgatgtgg ccttcgaccc
     9541 accagccgtc caagacgctg aggctgggga cgccgttgac ggcagccttc atgccgctcg
     9601 tgccggaggc ctcaagggga aactgcggag tgttgagcca gaggtcaacg ccggaggtca
     9661 tcaagccgcc gagggccatg tcgtaatcgt cgagatagac gatgcggagg tcccggcgca
     9721 gcgccttctt cgcgcggaag atgctgcgga tgatctcctt gcctccggcg tcgccggggt
     9781 gggctttgcc tgcgaagaca agctggaaag gaccggcgcg ggcggcgatc tccttgaggc
     9841 ggtcgagatc ggagaggatc agatcggcgc gcttgtaagc ggttgcgcgg cgcgcgaagc
     9901 ccatggtgaa gagatccgga tcgagtgaga gtcccgattt gacgtgcacg aactcgagga
     9961 ggcgacgctt ggcggcctgg tgcgcctgcc agacttcctc ctggggcaga ccgagagccg
    10021 cgcggaggct gaaattatct tcacgccagc cggggatgcg ggcgtcaaag agctgttgga
    10081 aggagggcga agcccaagtg gcggcgtgaa cgccgttggt gatggattcg atacgcaggt
    10141 cggcgaagag gcggcgggag gtggcgccgt ggaccttggc gacaccgttg acgtaactgc
    10201 tgagctggag cgcgagataa gtcatgttga gatttgcgcc ggcccggacg gcttcagcga
    10261 agtccttgaa gggcccgtcc tcctggacaa cttcgttgag caattcggag gagaaggcgt
    10321 cggcgggatc gaggaagtcg gagcgatgac gaaaagcccg agcgaccatt tccatgggga
    10381 atcggtcgtg gcccgccggg acgggcgtgt gcgtggtgaa gaggcacttg cggcgaacgg
    10441 attgaacgtc ctcggcgcgg atggagcggc gaccggctct tgcggcctcc tcattgagga
    10501 gctcgacggc gaggaagctg gcatggccct cgttcatgtg atatcgggag atcgtggagt
    10561 aaccgagcgc gcgaagcatg gagatgccgc cgattccgag aatgacctcc tggcaaaagc
    10621 ggtagtatgc gtcgccgccg tagagagtgc cggttagcgc gcggctggcg ggatcgttga
    10681 cagggagatc ggcatcgagc aaatagacgg ggatctcata gccggagacg ccgcgaacag
    10741 aataacgcca gcagcgcaat gcgacggggc ggccgtcgag ggtaacggaa gtgcgggcct
    10801 cttcctggcg gagaaagtcc tcgacgcgcc actcgacggg ctcttcgagc tgggtgccgt
    10861 cggattccag gcgctgggag aaatagccgc cgcggtaaag gagcgagacc gcgaccagag
    10921 ggactttcat gtccgccgca gcgcgaatgg tgtcgccagc gagaacgccc aggccgccgc
    10981 tgtaggtggg catggcgttc tcgagagcaa tctccatcgt gaagtaggcg atggagcgcg
    11041 agtcgaactc ggacggatcc gtcatcttcg atgattctcc gacgggtgcg cggcgagaag
    11101 cgcccaacac acagggggcg cggccgtgag gcgtctattc tcggggaatg gagaacgagc
    11161 cgcaagcgtt gcggagatgg agtgttcgcg gacagagggc tgcatcgatg agattaggac
    11221 gaactaaagt tcgctcctac acgagagcaa gacaagagcg ggctaggaat gggtggcgcc
    11281 cccgagctcg cggaggatat ttcccacgaa ggccttggcg tcgccgaaga gcatgagggt
    11341 tttgtcgaaa tagtagaggg ggttgtcaat accggcgaat ccagggctca tgccgcgctt
    11401 gatgaccatg acggtgcggg ctttgtccac attgagaatg ggcatgccga agatgggact
    11461 ggaagcgtcg gtgcgcgcgg cggggttggt gacgtcgttt gcgccgatga cgagggcgac
    11521 gtcggtctgg gggaagtcgg agttgatctc ttccatgtcc agcagacgat cgtagggaat
    11581 gtcggcttcg gcgaggagaa cgttcatgtg cccgggcata cggcctgcga cggggtggat
    11641 ggcgaatttg acgtccacgc cgcggcgggt gagggcgtcg tagagctcgc gcaccttgtg
    11701 ttgcgcctgg gctacggcca ttccgtaccc gggaacgatg acgaccttgt tcgcggcggc
    11761 caggattccg gcagcttcct cgggtgtagc gctccggacg gtcttcgctt cttggccggc
    11821 cgcgacggaa ggctgcacct tgccgaaggc cccgaaaagc acattggtaa aagagcggtt
    11881 catggccttg gacataatga tggagaggat taagccggaa gagccgtcga gcgcgccggc
    11941 gacgatgagg agcttgttat cgaggacgaa tcccatcgca gcggctgaaa gacccgcgta
    12001 agagttcagg agggagatca cggtgggcat gtcggctccg ccgatgggga tgaccatgag
    12061 cacgccgaat aggagcggta tgccgacgat caacgggaaa aaaggcttgg cgccggggtg
    12121 caggacgaga taaacggcca cgacgatcga gctggcgagc agggagagat tgacgaggtt
    12181 ctggcctttg taagtaagcg ggcgctgggg aaggagttcc tggagcttgc ccgcagccat
    12241 aaggctgccg gtgaaggtga gcgagccgag gatgacctca agggagagga cggccatcat
    12301 gaagcggggc acctgggggg agcggaggta gaactctgcg gtgccgacga gggtgacgca
    12361 gagagcgccg aaggcgtggc tgagcgcggt gcgctgcggg acggcggtca tctgtacgcg
    12421 gccgagggga actccgatga cggtgccgag gacaagagcg atggcgaccc acttgtagtc
    12481 cacgatgccg cgatggagga gcgtcccggc aatcgcgagg agcatgccga tttcgccggc
    12541 aaggacgccg cggcgggcgg tggcgggcga gttcatccat ttcaaagaga ggatgaagag
    12601 cgcgctggcg acgaggtaga cggcctcgat gatgtgctca gtgggaatca agctggtcat
    12661 gccctcagga gggtttcccc ggacggctgg acttgaacat ccggagcatg cggtcggtga
    12721 tcacgaagcc gccgacgatg ttgctggtgg cggcgacaat ggcgacggag ccgaggacga
    12781 cggagagggt gctcttgtgc tcgcccgcca ggacaatggc gccgacgaca gcgatggcgt
    12841 ccagcgcgtt ggtgagggac atgagcggcg tgtgcagcaa gggcgagatt cggcggatga
    12901 cttcgaagcc aatgaaaccc gccatcatga agatgaagag gctgtcaatc agatgcgagg
    12961 gcatctatcc tccattccgc ggtactccgc cgcagcaaac ggttctagcc gtgcgggacg
    13021 agcgcgggca atgagaaaaa ctcgcgaacg cgggcattga ccacttcccc gccccgggtc
    13081 agcagcgtgt cgcgaacgat ttcgtcttca agattcagca acagattgcc gtctttgacc
    13141 aggtgcaaaa gaaacgccgt gatgttgcgg gcgtacatct ggctggcgtg ataaggaacg
    13201 ccgctggcca tgttgatgcg gccgatgatg gtgacgccgt ggcggacgat gttctcgccg
    13261 ggttgcgaga gcgcgcagtt gccgccgcgc tcggcggcga gatcgaggat cacggaaccc
    13321 ggcgccatac tcttgaccat ttcctcggtg acgaggacgg gcgattcctt gccggggatg
    13381 acggcggcgg tgatgaccac gtcgctctcg gcgacgacgc ggcccaggag ctcgcgctgg
    13441 cgctggtaga aggattcgtc ctgggcgcgg gcgtagccgc tcgagtcctc ggcgtccttg
    13501 gcttcgatgg gaagctcgat gaagcggccg cccaggctca ggacctgttc cttggcggcg
    13561 gggcggaggt cgtaggccga ggcgacggcg ccgagtcggc gggccgtggc gatggcctga
    13621 agcccggcca cgccggcacc aatgacgaga acgcgcgcgg gggtgatggt gcccgcagcg
    13681 gtcgtgagca tggggaaaag gcgagggagc acccccgcgg cgacgagcac ggctttgtaa
    13741 ccgcagatgg tacccatgga ggaaagcgcg tccatgctct gtgcgcgggt ggtgcggggc
    13801 atgagctcga tagcgaagga agtgacgcca gtcgcggcaa tctgctgaac gatttcgcgg
    13861 gagccaagag ggcgcagaaa accgatgaga acctgatcgc gacggaaaag aggaaggtcg
    13921 gctttgccgg tgacgtcgtt ggagccgtag caaaggacct ggacaatgat gccggccgaa
    13981 gcgaagacct gggaacgctc gggaacgatc ttcgcgccct tttcaacata atgggaatcg
    14041 gggtagccag cttcgacgcc cgcgccggct tccacgagaa cctcgaagcc agtcttgagc
    14101 agattgggga tgacgatggg aacgagggcg actcgccgct cgccgggata gatctctttg
    14161 gggacgccga ggatcacttt gccgatttcc ttatgccgcg gctcattctg cggccagcct
    14221 ccggccatga agaaaacatc ctcaagtgtt ttccgagtcc aggacaactc tccgagaagg
    14281 gcatgcagag aaggccattc taccgtgcat catcggaaag tccacgagac gattgcctct
    14341 gaagaaacag agaaaaacct catggatcgg aattcagagc tcgtgcggcg gcggtctgtt
    14401 tcagagaggc caggtccgag cgcaccatca tctcgatcag ttcacggaat gccacgcggg
    14461 gctcccaacc caaggctctg cgggctttgc tggggtcgcc gcacagatac tccacttcgg
    14521 ccggacggaa gaggctggga tccacatcca cgtaatcctt ccaattgagc ccggcgtggg
    14581 aaaatgcagt ctcgagaagc tcccgcacac tgtgaggccg gccggtggcg atgacaaaat
    14641 catcgggctt gggctgctgc aggatcatcc acatggcgcg aacataatcg ccggcaaaac
    14701 cccagtcgcg ctggacgtcg agattgccca tcttgagcct gtccgccaat cccagtttga
    14761 tgcgcgcgac ctgctgggta atcttgcggg tgacgaactc ggtgccgcgg cgcggcgact
    14821 cgtggttgaa ggcaatgcag gaagaggcga agaagccata gctctcacga taattgacgg
    14881 tgatgtggtg cccaaagacc ttggctacgg cataggggct gcgcggatgg aacggtgtat
    14941 cttcgttttg tgatggcccc ccgaccttgc caaacatttc cgaactcgag gcctggagaa
    15001 atttcgcttt cggggcgtgc cggcgaatgg cttcgaggac acgcaagacg ccgagaccgg
    15061 tgacgtccgc ggtgaaagag ggctgattcc aggagacggc cacgtagctt tgcgcggcta
    15121 gattgtagac ttcgtccggt tggacggatt gcaaggcgac atcgagggac ccctggtcgg
    15181 tcagatcccc ttcgacgagc tggatgcggc cggcgacgga ctcgagacgg tcgtattttt
    15241 caagactgct tcgccggacc aagccataaa cttcgtagcc cttctccagg agaaactcgg
    15301 ccaggtacga cccgtcctgc cccgtgatcc cggtgatgag cgcgcgcgtc atggtcggtc
    15361 tgttcgccca gactttatag catgtcggtc aagagcgcca gccgaagcca acggatggtc
    15421 cgtttgaata agacagggag acgcagatgc tcgcgctttt cttcggatag gaaggcgaag
    15481 gctgaaccga gcgggattct gactgccgac cgattgctca tggcacgata agggcaacga
    15541 gaggccgggg tttccaggtc caagcaaacg cgcagaagga aactcattcg cggtccgctg
    15601 cttgtcgcct caatccttgg actggttaga gcacgttaag ggtgatggtg gcggttcccg
    15661 aatcccccgt tgatgcgctg gtggcggtga ccgtgatttg gaaggctccc gtcggagtcc
    15721 cgcgcggccc gccggcacat ccggcaaacg tcgccaggac aaacaccaca ccacccaggc
    15781 agagggccag tcgcagccgg gccttcttgc cctttactgt gccgaggccg attcccacca
    15841 accccaacag cgggagcaga actgcataga cgggagttgt tccttcacca ccgaacggtg
    15901 gagcggaagc cgatcccgcg ctggtggcaa tggtcagggt cacggtagca gtgagctggc
    15961 tctccgacgg cggattgaag ctgcaactgg cgccgggagg caagccgctg caggagaagt
    16021 tgatggtgcc ctcaccggcg ctggaatcca cggtaaaggt cgtggaagtg gaattgccgc
    16081 gactcactgt tagagtgctg acattgaaag taatctggat cggcggaccg atgtgggtct
    16141 gatcggaagc ggtatcgttg ccggtgttgg tctcgccgcc gccggaaacc gtggccgtgt
    16201 tggtaacgtt cgcctggata ttgagcggca cgttcacggt cagggtgatc ggcgggtagc
    16261 tgccggccgg ggccaggaca tcgctgcgcg tgcaggtcag cgtccccagg gtgcacgtcc
    16321 atcccgtgcc gctcatggcg gtggccacca gggtgttagg cacgttgggc aagttatcaa
    16381 ccacggtcac ggtgcccact gtgggcccct ggccggagtt ggtcactgtg atggtgtaag
    16441 tggcgccagt ctggccgcgc gagaagttcc cggtgtgcgt cttggtgatg gtgaggtccg
    16501 ccggctggac cgtgaggttc gcggtggccg tgttgccggt gttgccgttg gtcgagctta
    16561 ctgccccagt ggtattggtg aagttcccgg tggagctggc cgtcacattc acggtcagtt
    16621 tgcagatgct ggcggcggca actgtgcctc cggtcagcga gacactgccg aagcccgcgg
    16681 tcgccgtagg cgtgcctccg caggtgttgg tcagcccatt gggcgtggcc acgaccatgc
    16741 ttgccggcag agtgtctgtg aacgctaagc ccgcctcagc cacggtattg gccgccgggt
    16801 tggtgatggt aatcgtgagg gtcgaggtac cgttgaccgc gatggccgtg ggattgaacg
    16861 acttggcgat cgaaggcggc gcaaccacca cgacgctggc cgaggcggtt ccgcccgtga
    16921 ccggaaggaa ggtgccgctg ccatcgtcga aggtggcggt cacgttgccg gtggtgttgt
    16981 tcttggtgcc gccagtggtg ccggtcacgt tgatgaagaa agagcacgaa accgattggt
    17041 tcaagctcaa cgccgtcata ctgatcgtgt tgctgcccgg attggctacg acatcgcctg
    17101 tgacattggc gttcgggtca gcgcagccat tcggcagacc gttcggagac gccacgacca
    17161 gtccgctggg cagagtgtca ttgaacgcaa tgcccaccaa agacgtactg gcattcgggt
    17221 tgccgacggt gaaggtcagc tttgtgcttc cattcagcgg aatgctggcg gcgccgaagg
    17281 ctttggctac agtcggagcc tgaatcacgt caatggtcgc ggtattgctg accgcgccgg
    17341 tgccgctctc attggccgac accgccccgg tggtgttgct cagtgtcccg ctgccggaaa
    17401 cgagcaggtt cacgtttacc gtgcagaatc cggcggcatt aacggcatta cccttcgggc
    17461 ctactcgccg tgtgaccgcg tctccacgcc gtccccgtga aggaggcggc ggtgctcctg
    17521 gatctatagc gcctcccgtt aaactaatgg aacccggcgt agcggtcaaa gtgccgccgc
    17581 aatcggtgtt cagggtgttg aaattcaccg tcagccccgc gggcaaggtg tcgctgaaag
    17641 cgataccagt caggggcacg ttggggttcg gattgctatt tggattgacg atggtaatgc
    17701 tcaggaggac tgtcgagccc tgcccaatag cgctctgcgc aaaggacttg ctgatggtcg
    17761 gtggtgtggc cgcgttcacc gtgatgctgg cattggaagt gttgccggcg cccccctcgg
    17821 tcgaggtcac ctgaacggcg ttgttcttca ccgaagccgc ggttccagtc acgttcacag
    17881 agaaagtgca agaggcattc gctgccaggg tggcgcccga caggctgatg gtgcccgccc
    17941 cggcagttgc cgtaatcgtt ccgcctccgc acgcacccgt caggccgttg gggcttgcta
    18001 ccaccagccc tccaggcagg ttgtcggtga acgcaactcc ggtcagagac acgctcgtgt
    18061 tcggattctg gatcgtgaag ctcagcgtac tgttctgact cagaaagatg gtaattggat
    18121 tgaaggactt gacgatggtc ggtggtgtgg ctaccgcgag gttcgccgaa gccgtgttgc
    18181 cggtgccgcc attggtggag ctgaccgcgc cggtggtgtt ggtgtagttc gccgatgccg
    18241 tgccggtaat gtttaccgtc acggtgcagg tgctggaggc agccacggcc cctccggtca
    18301 gcgagatgct gcccgagccg gccacggccg tggcggtgcc gccgcaggtg ttggtcagcc
    18361 cgttaggcgt ggccaccacg agtccggcgg gtaatgtatc cgtgaacgct actccggcct
    18421 cggcgaccgt gttggccgcc ggattggtga tggtgaagtt cagcgtggtc gtgccattca
    18481 ccggaatcgc cgccgcgcca aacgctttgc tgatcgtggg cggcgccacc accgtcaccg
    18541 tcgccgtgtt gctgggcgca ccggttccgc cttccgtgga ggtcaccgcg ccggtggtgt
    18601 tgctcttgct tcccgcagtg gtgccggtca cgtcggccga gaaggtgcac gatgatccgg
    18661 ccgccggtga ggcagtaagc gttcccccgg tcaggctgat ggtgccgctt cccgcgaccg
    18721 cggtaatggt gccgctgccg caggaaccgg tgatgttcgg cgtagccgcc accaccacgc
    18781 ccgaaggcag ggtgtcggtg aagcccactc cgctcagcgc gctggcggtg ttgggattgg
    18841 tgatggtaaa gctcagtgtg ctggtggcgt tcaacgcaat actggttgga ttgaaggcct
    18901 tgctgatggt gggcgggccc accaccgtaa tgctggcgga agccgtgctg ccggtaatgc
    18961 cgccggcatc ggtggcggtc acgttcccgg tggtgttgct cttcagccca gcggttgtcc
    19021 cagtcacgtt catcgttact gtgcaagacg ctcccggtgc caggcttgca cccgaaaggc
    19081 tcacagtgga cgcgccatcc ggtgcgctgc cggtaccgtt gcaggtggtg ctcacattgc
    19141 cgggtgtcgc cactaccagc cccgccggga acgtgtcgct agccgcaatc ccgttcagcg
    19201 tggtgttgct gttggtgttg ctgaaagtga agatcagact ggtcgtgccg ttgagcggaa
    19261 tcgtggcggc gccaaacacc ttggcgatgg tgggcggatt gatgaccgtc aggctggccg
    19321 aagaagtaga ttgagcaccg gtgccggccg cggtggaatt gatggtaacg ctgttggggt
    19381 aaacgctgtc ggtggcgctt tgcacatgta ctgtgatggt gcaacttcca actgccagag
    19441 aagcgttggt gaaggtcacg ctggtatcgc ccgccgccgg cgtgaaggtg cctccgcagg
    19501 tgttagtaac gctgggcgga ttggcaacca ccaggttggc cgggaaggtg tccgtgaagc
    19561 tcgcatccac cgcgatcaca ttgggattga ctacgctgaa ggtgagagca gagggggcat
    19621 tcacggcaac cgtggccggg ttgaagctct tcgtaatctg gagaggctcc aatatgatga
    19681 tgccgacatt ggccaagcct ccgcttgacg cacccagacc cggcgtaggg tcagtgtaag
    19741 taaaggttgt tgccgtgggc gtagctgtga ttgcgaatgt gccatcgtag ccacccacgc
    19801 cgacgcccga aataacgaca gtctcgcctg cggcgaagtt gtgcgcaccg accgtggtga
    19861 tggtaacggt gttaaccgct tcgctggcgc tggcaatcag attggaattg gcggagacaa
    19921 cggcctggtc cacggtggca tgggcggatc ctcgccctga cgcaccaccg gcattgaagg
    19981 tggccgttgc ctgcgcactt gcattcagcg tttccggctg cgcctgagga atggttccca
    20041 gaaccgggtt gtcaaaggtg atgggcagcc cgacaatcct gtccagattt ccgcttagag
    20101 cggcgccgtt gccatggttg tcttggctca tgtcgccggt caaggtcgtg gttccgttaa
    20161 ttttgatgat tgccggggtg gcgtcgtggg tcagaacgat gaaggggtcg aacgtcgtgg
    20221 tcgcaccggc gatggtcgtg ttgatcgtgg tggcagcggc gttggttccc caccagttgt
    20281 tcgtcgctgt gactgtggtg ccgctgttgc tgagattgct gccacttccg ccggcggtat
    20341 tgcccgccag ccggctgaag gtgatcgaca tgggaccggc agtgggaaac gtgccaccgg
    20401 atccggtgtg gatcccacca ccattgccgg tggtgctgtt gttgatgatt gctaccttgt
    20461 tgaaggtgac ggtattgggg aaggagcagg aaccgggaca accgaaggcg ccgaggctga
    20521 gatacacgcc tccgccgtcc ttagcgttaa caacgttgcc cagcccgccc ttattgccgc
    20581 tgatgaccgt gccctggtca acgataaggc ccatcgctat gtccgacacg ccgccgccga
    20641 agccggctgc ctggttcccg gaaatggtgc tggagtggat cgtagtggaa ccccaaaatg
    20701 gcggcgtggc acagcaggcg tcgagagcta tgcccccgcc cagccctgcc tggccgctta
    20761 ctacgttggt cgaggtattg ttctgcacga gggtgttggt aatggtgacc agcgagcccg
    20821 cgctgatagc gattccaccg ccctccgtgc cagcgaccga gtccacagcg ctgttgttct
    20881 ggaaaacgct gttggagata gttactgtcc cgccgcccgc cggattcacg aaggcgaagg
    20941 tcacaagccc gccgccgccg ccctgcgtag tcgcgcaatt ctgaatggtg acgttggtca
    21001 acgaaagggt cgcagtgccg ctggtgccgg tgtcgaactc catgcagccg ccgtcaccgt
    21061 cgttgttgtg cgtcccgtgg tttacgccat tttgaatggt cagactggag atgctggcgg
    21121 tggcagtggt agtggtcggg acagtcgcgc ttggaatatc ttcattgacc gccatcacca
    21181 tgtccacggg gccacctacg ggcgttcccc aagtgatgat ggtggtgttc tggccggcac
    21241 caatgatatt taccgagtcg tttatgttta aagtgcccgt ctgcgcatca tagagaggcg
    21301 tggcggcccg tccgcgcgtg agcgagaatg tgcctgcagg gaccatgatg gtgtccgtgc
    21361 caggagtcgc gttggcctcg atgatcgcct cacgcaaact gcaaccgggg agcgccgctg
    21421 cgcaagctcc aggagtaaga acatccagcg tcgtgttgac tgtaaacgtg gggtctggga
    21481 tgggcatcac cataaaaggc gcgatttcgc cctgatgcag cgccatgatt cctggacggc
    21541 cgtccacatt gatgcgcatg ggcagggccg ccactggcga gccggagtaa ggctttagcg
    21601 agagctgccc gggaagaaaa gtgcttgcgc cgggaggcac gtcgggatgg gagatgacag
    21661 ccatttgtcc ggtggcggcg ctgagccaca tgatgtcgtc ggcgccgtta ctcgagacgc
    21721 gggtgcggaa gaacaccgga gcctggctgg agctgatgtt ggcgaccgag ggaaagccct
    21781 ccacgatcct ccacccgttt acagggaatg acggtgccgg gacaaaagaa gggggcggct
    21841 gatggttcag cttggcctgg cgaatggcgc tgaattcctc caccgtatag acgcggggat
    21901 caaactcgta gcggacggca atctgaatac tgccgtcaga ggccagcagc gcgatttgcg
    21961 atccggaatt gcggtcataa ataaacgaac cgagggcaaa ggcgctggcg gaaaccggca
    22021 gcgaaaccgt tgccagttgc atgttggagg agcgcagaat ctggatgtgg ccgccggaga
    22081 tgaaagctgc gtccgggccg ccgccgaaat cgccgaaaag aatgttcgag gccggcgcgc
    22141 tcaggggata actggcaagc gccccgagcc ccactcccgt ctcgccgaga ataacgaaag
    22201 aaaatgaatt gttcggcccg gaaatgccaa ccgccaggtt ggtgagagag tggccgtttc
    22261 cgaattcgcc cgccgccagt gccgtgattc cgcccgtcaa agtaatgatc tgaggattcg
    22321 cgaacttgcc cgtcccgtcg cctgcaaaca agtagatcgc gttccccccg cgcgcggcca
    22381 ccaccaggtc caaatcgccc tggccggtga aattcccgac cgcgagaaag tccggccgga
    22441 tcgccagtct tgtaacctgc acgccaggaa ggaacggtga cgggaattcg ccccggccaa
    22501 tggcttggaa cgacgcgtcg ctttggggag cgaaggcatc caggttgccg cggtgaaagg
    22561 caagaatgcc gccacccgga acggcgtaac ccacgaccaa gtcgttcacg ccgtcgcggt
    22621 cgaaatcgcc cgcgatcatt gccagcggct ggccctgtcc gctggccaga atttgcgccg
    22681 cgccgtcgtt aacgttctgc gctgcgacgg tggccccgct cgacgctccg acggcggccg
    22741 ccacgtactt caccggcagg ctccgcggct cctgcagcca gatcctgggg ccgctcggtg
    22801 tgttttgctg ggacgcgagc ccggtgacga acaccaggca tgcaagcaac ccggcgcaga
    22861 agaatgagac cgcgcgaaaa ccagccggaa gacgaagtcg gcccatggga aatccacctc
    22921 cacccgcgag acttaaacac ctgaattttc agtcgatccc aagcgcagcg gcagacacca
    22981 tcagagggcc aagtcaccgg aaagatgccc tcacgttgcc ccgcaaaaga gacgtagtgt
    23041 ttcatgagcg cacgccaaag tcaattccgc atgcgtactc tgaacgatcg aaaaattgtc
    23101 cccgcggagg gttggagcct gccgggctcc ttcgaacgac aggctacagg cccaggtagg
    23161 ctgcgcgcac catctcattg gtgaggagat ttgcggaggt atcttcgagg agcacagtgc
    23221 cgttctgcac cacatagccg cggtctgaaa tgcccagccc gctcaccacg ttctgttcca
    23281 caatcagcat ggtgatgccg gtgcgccgca cttcctgaat cttctcgaac atttcttcaa
    23341 caatcttggg cgcgagcccc agggaaggct catccaacaa aagaagtttc ggctgcgcca
    23401 tcaagccgcg ggctatggcc accatttgtt gctcgccgcc ggagagcgtg ccggccagtt
    23461 gatcgagcct atccgccagc ctgggaaaca gagcgagttg ttccgccatc aggcgcttca
    23521 catgagtacg ggcgcggggc gtgtaggcgc cgagcgaaag gttctccctg acggtaagga
    23581 aagggaacac ttgacggccc tcgggaacat gactcaagcc gcgggcaacc atctcatgcg
    23641 ccgggacatg ggagacgtcc tcgccgtcaa agcgcaatga cccgccggcc agttgtttta
    23701 ttccggagat gaggcgcagg agggttgtct ttcccgctcc gttggcgccg atcagcgtca
    23761 caatctcgcc ggcctggacc cgcagcgtta tcccaaagag cgcctgcaaa tcgccgtaga
    23821 aggcggttgc gtctttgagt tcaagcagcg gcttgccggg aggccggctg gcagcgccgg
    23881 cggggttcga cgaacttgaa gccatcatcg cgccagctcc ttgtatttcg gacccaggta
    23941 ggcttcgatg accacgggca gattcacgac ctcagagggt ttgccttcgg cgatcttggc
    24001 accgtaatcg agcacaacga tgcggtcgga gatcttcatc accacctgca tcacgtgctc
    24061 gacgccaccg acaacgctga ctccccattc cggcaggcgc ttcaccaggt tggccagggc
    24121 cagagcctcg ctggtggtca agcccgacac gacttcgtcc aggaggatca gcctgggctt
    24181 agtggccagg gcgcgggcga tctccagccg cttgagcgcc gccaacggca atccacgcgc
    24241 gggggaatgg cccaggtgcg acatctgcag gaaagcaaga attctcccgg cttcttcttc
    24301 tgccttatgt acgttgccgg agtgcataaa ggcccctagc atcacgtttt gcagcaccgt
    24361 taaattcggc aagggcttga cgatctggaa tgtgcggccg atccccaggc gtgcggcgcg
    24421 gtgcggcgcc agccgggaga tatctttgcc ctggaagagg atggctcctt cctcgggatg
    24481 cagaaacccg ctgatgaggt tgaagaccgt ggtcttgccc gcgccattcg gtccgatgat
    24541 gaacaggatt tccccttcat tcagtgtgaa ggatacgttc tttacggccg ccagcccgcc
    24601 aaagcgcttg cagacattct ggagttcaag gatcggcgtc atcggctagt ctcctcaagt
    24661 gccgctggcg ctttggagtt tggaggaccg gccgcctccg gagccgcagg aggcttaatt
    24721 cgcttgcgga gccggtgctg caacccgccg acgatgcctt ccggcaaaaa cagaaccacg
    24781 acaacgatca agatgccgta aatcaggaga tggctttcct tgaactggtt cttgaacacc
    24841 tccgatgcag acagcaggag gaccgcgccg acgatgggac cgagaagagt gcccgcgcca
    24901 ccgagcatgg taataatcgc gatttggatg gagatttcaa ggctaagaat gctgtccggc
    24961 acgatgaact gaaatgcact cccgtagagc gccccggcca gcgccgtcag aaaggcgctg
    25021 atcagcagcg accgcaactt atagcccgcg gtatcaatgc ccacggcgct ggcggtttcc
    25081 tcgtcttcgc gaatggccat caggtagtag ccaaaacgcg agcgggagac ccagtaattc
    25141 accaggaagc tcgcgagcgc caaggcgacg gcggaaaggt aaaacaagtc ccagaacgtt
    25201 ttcccggtga agagcgcagg ggtggtaagc ccaatggccc cgccggtcac gttcaagttg
    25261 gtggccacca accgcagcac ttccgcggag gcgatggttg ccagcgtgaa gtacggaccg
    25321 cgaaggcgga agacgatccg gccgatcacg gcggcgaaga gagtggcgac aatcgccgcc
    25381 aggaacattg aaatccagct ggggatggag agtgcggtga agagcgcgag cccgtaggca
    25441 ccgagaccga aaaaagcaac gtggcccaag gagagctgcc cggcgtagcc acccactacg
    25501 ttccatgcca gtcccagggc cgcaaacagg gcgatacgga agagtacctg catctggtaa
    25561 tcgttctgga cgaatagcgg agtggcggcg catgcgatca cggcgccgag cagtgtccag
    25621 agcttccagc tcttcaagct gctcatacgc gctgtctccc gaaaattccg ctgggcttaa
    25681 acgagagcac cagtagaaaa atggtgaaat cgaccaccag cgcccactgg ttgccgagat
    25741 agaggctggt gaggctttcc gccaggccca ataccagccc gcccagcgcc gctccttcga
    25801 tggagcccat gccaccgagc acaacaatca cgaatgcttt tgtcgtgtag tcctctccca
    25861 cgctggggaa cagatagagc gcaggaagca gaagaccgcc ggccatcccg gcaagagcga
    25921 ttccgagcgc gaagctgaaa ccatggatgc gcgacacgtt gatgcccatc aagggggcca
    25981 cctcggggtt ctgcgcaatg gcccgcaagg cttgtcccgt aaggcttctg gtcacgaaca
    26041 acgtcaaacc ggcgattaag acggcggcaa tcgcgaaaga cagcaaggcg gcggcgttgg
    26101 cggtgaacgg accaaaacgc agggtacggt ttatcagggc gatgtttgtc tggcgatagt
    26161 cggcgtgaaa gataagctgc gcgctgtcgg tgaggatgag ggcgatcccc gtggtcatca
    26221 atatctgcat gaagtgcgac tggttgcgca ccggcttgat cacaagctgg tagacgagat
    26281 aaccgaagag gaaaaggaag agcccggcgg gagcgaatcc gaggtaggga ttgatcccca
    26341 gtttgtcaaa gaggacccag gtcgcgtaca tgcccaccat gagaaaagcg ccgtgcgcga
    26401 agtttacgat gcgcatgaca ccgaagatca gcgccaggcc catgccaatg agcgcgtaaa
    26461 gcgcgcccgt caggaggccg ttcatgcagg cttgtaagaa ggcgatcaac gcgtgcgaac
    26521 ctccgctgtt caattccagt cccggccccg cagcggcgct ctgacccgat tggctacaac
    26581 ggtcccggtg ctatcgctgg gaccaacgag gagcagggat aatgggcttg ctgtcggcgg
    26641 cgttcgcggg atagacaacg cgataccgcc cgccctgcac ctgcgtaatc aagaccgggt
    26701 gctgattctg gccgttggaa tcgaaccgga tcgggccaaa cggagtggaa gtgaggttga
    26761 gattcttgac ggcatcgcga atcttagcgc tgtccgaaga tttggcatcg ttgattgcct
    26821 ggacggcgac gtacagggct gcataagctt ctatcgcatg ataggccgga tgcgagttgt
    26881 atcgtttcgc atactccgcg tcgaactcct tggagccggc ccactgcgcc tccggcagcc
    26941 actgcgagac tgaaaacgtg tattccgcgt ttttgcccgc gcccttcggc gtgggaaact
    27001 cggcggccgc aaatcctgta ccggcagaag tgtaatacct gggattcagg tcaagctcct
    27061 gcgcctggcg cagcagtgtt cccgcatcga gcaaatagga acagaaataa atcacctcag
    27121 gattcgcctt tttgacccgc tgcaacaccg ctttgtagtc cggcgacttg gcctcataag
    27181 actcaaccgc cacaagattg atgcctgcgc gctgggccgc cgcggtcatg gctttcatat
    27241 tcgactggcc gaagttggtg ttctcgtaca cgatggccat ggtcttggga gcgccggagt
    27301 ccttcagaaa cgcaatggta gttctggcgt aatcggtcgc tccggcgcag acgcggaaga
    27361 cccaaggcga tttcgactcc atcacgttgt cggcggtggc cgtcggcatg atcagcggga
    27421 cctgcctctg cgtcacggaa gggatgatgg ctttggtggt ctcgctcgaa tacgaaccca
    27481 gaaggatggg gacgtggtcc tgatcgacga gcttggaaac tccctgcacg gcctggtcag
    27541 gcttgctctg atcatcgtaa aaatccagct cgatcttctt gcctagagcg ccgcccttgg
    27601 cgttgagttc atcgagcgct atggagtagc cgtgcttgtg agcttctccg aaagccgcca
    27661 ggctgccggt cagggaggtg atgacgccga tcttgatggt gtctccctca ccaccctttt
    27721 tctggcaact gacggccaga gatatgccga taaagactac gagagaagct gcaactgtct
    27781 tggaacgcat aacgcctccc tatcgggtgc cagggccgtt ccgacttgat gcgcctgcga
    27841 gaccaaacga ttatcgcccc aggcggacgg ccggcgccgc ttaactgcaa gaataagaac
    27901 gacactagct cgatcgccgc cgcattatac acagcgcctg aatgaagctg tcagcatatt
    27961 tctgactggc tcggttggat gggggaattg gctcaaatca cctgggacag atggtgagcc
    28021 gggcaggact cgaacttgca acccgctgaa aaagagaccg tccaaagaca actgagaccg
    28081 aatgtgatga tcgctatttc tatgtgacat atctcacagt aggttgtgat tcgggtcaca
    28141 tcttctaatc gagcttgcgc ccagcatagg ttgattcggg gcattgattc cgaaagagac
    28201 cagcgatggc gagcgttctc ggggcatatg gttaccagtt gacagccccc ggcactccat
    28261 tgcagcgata tgaattcccg gcgctcgatc ccggccctga cgaagtgatt gtgcaggtgg
    28321 ccggctgcgg cgtctgccac accgacgtcg gcttcgcgct cgacggcgtg ccgacacggc
    28381 atagtctgcc acttgtgctg ggtcacgaaa tctcaggccg tgttgtcgca gcaggcgaaa
    28441 tcgccggcgg atggcttggc cggtccgtga ttgtcccggc ggtgattccc tgcggggcat
    28501 gcgcagcttg cctggcggag cgtccgacca tctgcagaaa gcaattcatg ccaggcaatg
    28561 acggcaacgg cggcttcgca tcgcacatag ttgtgcccgc ccgaggtttg tgcgccgtcc
    28621 ccgagaatct tccgtccggc ctcccgctcg agatgctttc cgttgttgcc gatgctgtga
    28681 cgactccgta tgaagcgatt cgaagagcag ggctcgggcc ggaagatgtg gccgtagtcg
    28741 ttggagtcgg aggcatcggc gggttcggag tgcaaattgc agccgcgttg ggcgctgcgg
    28801 tcgtagccat tgacattgac caggagagac tcagccaagc cgcccgccac ggagcaggac
    28861 tggtcctcaa tgcacgcatc actgacctaa aaggcctaaa aggagcgata cgcgctttcg
    28921 ccaaagaggc cgggcgcaag ggcatcggct tgaagatctt cgaaatgagc ggcacgcccg
    28981 ccggccagac gaccgccttt ggactgctgg accacggcgc atatctggcg attgtaggtt
    29041 ttacggcaaa gccggtcgag ttgcgcctat ccaacctgat ggccttcgat gccaccgctc
    29101 gagggaattg gggctgcccg ccggaccaat accccaaggc actgcggttg gtcctcgagg
    29161 gcaagatctc acttgcgcca ttcacagaac agcacccact ggaggaagcc cccgcgattc
    29221 tcgaggcggt cgcgaggcat gagcttcgac ggcgggtgat tctgaggccc aaccacggca
    29281 agggaaaacg cgaatcggag ggagcatgat gaaggatcac aacctcgtcg aggctgctcc
    29341 tccacgaaac atcctcgtgg aacgaaagcc cgcacgcgat cgatccgggc agcgggtcga
    29401 atctctccac aatgtttgga tcaccctcaa caatcccaag gaactcaata gctacaccac
    29461 agacatggtg aaagaggtga tcctggcatt gcgcgaggcg agcaacgatc gcgcggccgt
    29521 ggtggtggtc ctgacgggag cgggaacgag ttccttctgc agcggcggaa acacacggga
    29581 atacgcggag aactatgcgg gcgcgccggg ggagtaccgt caatatatgc ggctgttcaa
    29641 tgacatggtg acctcggttc tgcaatgcga caagccggtg atttgccggg tcaatggaat
    29701 gcgcgtgggc ggggggcagg aagtcggcat ggcctgcgac ttttcgatcg cgagcgactt
    29761 ggcgtttttc agccaagcgg gacccaagca cggctcggtc ccggatggag gaagcacgga
    29821 ttttcttccc gtgtttgtgg gcatcgagcg cgcgatgcac agcggcactc tctgtgagat
    29881 gtggagcgcc caccgcttct actggctggg cgggctcaca caaatcgttc ccgcactgag
    29941 agtcggcgac aagtgggtgg ccaatccgct ggtcgtcacg gaccgggttc tcgatgaata
    30001 cgggcgcttg gctcttgggg agtggaagat ggggcaagcc cgcgaagaag ggaaagcact
    30061 catggccaag ggcaccgtgg atctttccct gctggacgaa gcggtcgagc agacctgcac
    30121 aaagctcctc tacaccatgc ccgattgcct gatcaagacg atcgagagcc tgcgcaagca
    30181 caaactcgag cactgggaca aaaaccgcga gaccaaccgc gcctggctcg cgctgaacat
    30241 gatgacagag gctaatgcgg gattccgcgc ttttcattac ggaaccaaag aggagcgcga
    30301 ggtagatttc atccggcttc ggcagcggct cgccgagggc gcgcgatggg gcgaggaatt
    30361 gatccgtgaa gtggctccgt gggtggcagc gtgcgcaaag acctctaagt aattgcgcga
    30421 gaatccggaa agccgctgga actatggagc aagggagttc tgaatgaaac ccgaattgac
    30481 cgagctcgtc agaagagcgc gagaactctc ggatgacctg gacttgaaga cggtgagcgc
    30541 ctggaaagag gcggtgcccg gacgcaaggc cgtcggccac atgcccatct acgtcccgcg
    30601 cgagctgatc cacgcagcga agatgcttcc ggtgggaatc ctgggcggcg gagaccgcct
    30661 ggaaatcatc cgtggcgacg cattcttcca gagctatatc tgccacatcc cgcgcagtac
    30721 gatcgagttg gcgcttactc accgcctgga tgcgctcgac ggaatgcttt tcccctccat
    30781 ctgcgatgtc atccgcaatc tctccggaat gtggacgatg cttttcccgg atcgctacgt
    30841 gcgctatgtg gacgtaccgc agaactacga accgggcgtg ggcggcctgt attggcggca
    30901 agagctcgag atccttcgcg aagacttccg gcgtctgagc gggaaccccg tttcggacga
    30961 tgatttgcgc cacagcatcg ccgcatacaa cgagaaccgc aaagcgatcc gcgatttcta
    31021 tcaagcgcgc ggtcgccagc cctggttcta tccgatttcc gaagcgtatc tgttcttgcg
    31081 cgcgggcaat ctgctgccgg tggaagaaca cacggcgctc ttgcacgaat acctgcgcct
    31141 ggcaccgctc gaggacgacc gcgtagaaca ggatgagagc cgtgtcgtgc ttgtcggcat
    31201 gttttgtgag cagccgccgc tcggtttgct ggccacactc gagagagccg gctgccgagt
    31261 cgtggatgac gatttgctcc tcggcctgcg ctggattacg agcggtgtct ccacgggggg
    31321 cgaccctgtg caaaacctgg cagaagcctt cttgaaccag agtgcgacaa cggcttcccg
    31381 ttatttgccc gacgggaagc acgggctctc gctcattgac tcggtgcgac gcaatcacgc
    31441 cgagggtgtc atcttctgcg cacccagttt ctgcgatccg gcgctgctgg aacaacccat
    31501 gctggtggct gcgctggacc gcgaaaagat tccccacacg cagttcaagt attccgagga
    31561 taccgggcaa tttgccgtca tccgcgaaca ggccggcaca ttttccgact cgattcgcct
    31621 atggagcgaa gcatgagcag cgcaccacag aagaaattgc ccgcgcagga cgtggctcag
    31681 aaagagcgca gcatgttgct gcaaaagcag atgattgcga gtcactacga cgcgctggcc
    31741 gccgcgcacg attccggccg caaggtcgtt tatacctttg tgcccggcaa tctcaccgag
    31801 ctcatgcgct ctttcgatgt cctgcccgtt cttccggaaa tcaacgctct gcaatccgga
    31861 atgcgcaagc tgtccggcga gtatatctcg gaggccgaaa aatacgggca cagcgaagac
    31921 gtctgcacct acgtgaagtg cgatatcggg atgttgaaaa agggcaatat cgggcccacg
    31981 ggaaagaagc tcccgcctcc cgatctgctt ctgctctcct acaccgggtg cttcacgttc
    32041 atgaaatggt tcgaactgct gcggcaagaa tacgattgcc cggtggccat gctgcacgtg
    32101 ccgtaccagt ctgacggcaa gatccacagc caccacctcg actatatcct tcagcagttg
    32161 cgcacagagg tgatcccgaa gctggaacag gtcacggggc tgcgctacga cgaggatcgt
    32221 ctgcgcgaaa agatgaagct ctccgctcgc gccgaggatg acttcgtatg ggtgcttgaa
    32281 agcgcgaaaa acatcccttc cccgattgat gcgtacttcg gcggcgtcta ttacatcggt
    32341 ccgatgttca cggcattccg cggaacgccg gagggagtcg agtactaccg gacgctgcgt
    32401 gaagaaattg aagagagagt cgcgcagcat cttggcccaa tcactccgga tggccccctt
    32461 gagaaggaac gcttccggct ggtcatggaa ggcccgccca actggaccag cttccgtgaa
    32521 ttctggaaga tgttctacga agaaggcgcg gtgatcgtgg ccagcagcta taccaaggta
    32581 ggcgggttgt atgaccgcgg ctttcgtcac gatccggaac ggccgctgga aagcctagcc
    32641 gagtactgtc tgggctgcta tacaaacctc ggcctgccca cgcgcctggg tctgctcgaa
    32701 cgctacatcc gcgattacca cgcggacggc ttcatgatca atagcgtcaa gagctgcaat
    32761 tcgttcagcg tcggccagct cctgatgctg aggcagctcg aagaacgcac cggcgttccc
    32821 ggcggcttta tcgaaagcga tctggttgat ccacgctatt tttccgccgc caacatcaag
    32881 aaccgcattg aaagttatct gcagatgctg gaccaaaaac gcggcgcgcg agccggagtg
    32941 cagccgtgaa ctcgatgaga gtattcgtag gaatcgatct gggctcaacg acgacgaagg
    33001 ccatcgtgct gggcgaagac ggcaacgtga tcggccgggg catcaccaat agtcgcagca
    33061 actacgacgt ggcctgcaac atcgcccgcg aagaagcttt tgtccgcacg cggttcactc
    33121 tcacgcggca agccttggcc tccgacgctt cgctggccgg aatcgaaaag tcgttcctgc
    33181 cctccttgga acgcaatttc cggcttcagc agcatctccg tcagctcgag cgtttgcgtg
    33241 aagcgcttct tgccggggcg gaaggcacgc gttacagcga gtcgaagcgc gaactgattg
    33301 aagtggtcga aggaatcgtc gccgccatgg cccagcgcgc acaggtccac ttcggcgaac
    33361 ttgcggtgcg caagagcgat ttcttccgcg accttgccgc cgcagattac atgcacctcg
    33421 ccgaggagcg cagcaaccgc aaaggcatcg ctttcgacaa gctctgcggg ctgtttgacg
    33481 cggcgatcct ggtggtcgaa aacgaatcca tcgaagaggc ctttgagcgc cacattgggg
    33541 ccgcgctcga ccgcactctg tctaccgcaa cacccgcaat aaaacagaat ctcaacagcc
    33601 ttcgcgccgg cgtcgaagcc gcgcgcgcca tccggctcgt ggaagcagca tcggtcggca
    33661 ccggttacgg ccgccagcgc ctgcccttcc caaaggaaca aatccggagc gaaattctgt
    33721 gccacggcct gggggcacat gcgatgttcc cggggacgcg aaccgttctc gacatcggtg
    33781 gacaggacac gaaagcgatc caggtggatg cggaagggat cgtcaccagt tttcagatga
    33841 acgaccgttg cgccgcgggg tgcggtcgct acctgggtta tatcgcggac gaaatgaacc
    33901 tgggcatcca cgaactgggc ccgttagcgc agtagagcac ttgccgagtc aagatcaaca
    33961 gcacgtgcac agtgtttgcc ggcgcggaat tgcgcgagcg gctttccctc ggccaggcgc
    34021 gcgaggacat cctggccggc ttgcaccggg cgatgatcct tcgcgcgatg tcactgctgg
    34081 cgcgctccgg cggaattgcc gatcagttca cctttaccgg cggagtggcg cgcaacccgg
    34141 cggccgtaaa cgctctgcgg gagctcgtac aagagaacta cggcgaacga atgttgaata
    34201 tctccgcgga ttccatttac acgggcgcgc tgggagcggc gctcttcgcc cggcgctccg
    34261 ccgaaagcgt gcattaatgg cgaccacagc gcaattgccg cctcgcgcgg aagaggctct
    34321 ggtcacggcg ggtgtggacg tcggctcgag ctcggtaaag atcgcgatcc tgcagatgaa
    34381 tgcaggcgat tccgtgcgag tcctcgggct gcgcatcgac cggattcgcc ggcgagatct
    34441 tcgcaaggtc atccacgaag gttttgaagt cgcgctggag gaagccggcg tcaagagatc
    34501 cgacgtggac tacgtcgcca gcacggggga aggcgagctg gtggatttcc gccgtggcca
    34561 tttctacgga atgactacac atgcacgcgg ggcgcttttc ctgttgccgg agacccgagc
    34621 ggtgctggac gtcggcgcac tgcacgcgcg cgccatgacg attgacgaac gcagccgcgt
    34681 cctcaactat cgtatgacca gccagtgcgc gtcgggttcg gggcagttcc tcgaaaacgt
    34741 ggcgcgatat ctcggcatcg cgctggaaga cgttggccct ctttcctgcc gggcgacaaa
    34801 tcctcagtcg gtcagcagca tatgcgcggt gctcgctgaa accgacgtga ttaacatggt
    34861 cagtcgcggc atttcgactg cggacatcct gaaaggaatt catctgtcca tggcgggacg
    34921 ttaccttaaa ttgctccgct cgattgatgc tacaggccat gtggcgatca gcggcggcct
    34981 ggcagcggat actggcctcg cccgcgcgct cgaggagaaa atcaaggaag aaggtctgag
    35041 tttacaggtt cacacgcacc cgcagtccat atttgcagga gcgctcggcg cagccctgtg
    35101 gggcggatat cgccatcgag tccttgcgcg ccgggctgcg gtgcgggctt gagaagcacc
    35161 aaggagaacg atcatggaac aagcgccaat ggaaatgccc ggcactttgg aaacggatgc
    35221 cgttgtggtt cgcacgatgc gcgagaatga ccttgaggca gtcgtctcga ttgacgctgc
    35281 cgccgctggc cggcgccgcc cgcgttattt cgagttgatg ctgaagcgcg cgctgacgca
    35341 aacaggagtt cagatctcac tggtggcgga aattggcggg cgcgtggtgg ggttcctgat
    35401 cggttcgctc tactacggcg aatatggcgt gatggaacca tcggcatcca ttgatgctat
    35461 cgcggttcac ccggattttc gcggccagca cgtgggaaaa gccttgatgc gacagatgcg
    35521 gctgaatctg ggcgcgctcc gcatcgccgt gctgcgcacg gaagtggaat gggacgactt
    35581 tacactggcg actttcttcc gcagcgaagg cttcacaccc gccagacggc tgtgtctgga
    35641 atgctccctg gatccaacct caccacggga ttgaagaatc gccgaagcgt ttcgacagcc
    35701 acagctgagc gaatggaccg gcttcccaga tatggcactt ggattctcac ctcatgtgcc
    35761 ggcaacggag aggctgtagg tttgaatcct acccggaggg ccaaattctt gttttccggt
    35821 gacttggatc tttcagaagc ctgattctct ggtcaaagag cattcggttc tctgccgaat
    35881 gcaggcgctc gaacggtcct caatcgagcc aattttgacg gagacggggc gcaaaattgg
    35941 gcacagtttt ttatttctga aaagccagac aaagctaacc tattgaaaag aagtggtgag
    36001 ccgggcagga ctcgaacctg caacccgctg attaagagtc agctgctcta ccattgagct
    36061 accggcccac gatcgg
//
